LOCUS       DAZFWP020000001        90850 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFWP020000001 DAZFWP020000000
VERSION     DAZFWP020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 90850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.001"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            39..623
                     /gene="yraH"
                     /locus_tag="ACHK0Z_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             39..623
                     /gene="yraH"
                     /locus_tag="ACHK0Z_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4500090.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            703..1398
                     /gene="yraI"
                     /locus_tag="ACHK0Z_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             703..1398
                     /gene="yraI"
                     /locus_tag="ACHK0Z_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4500091.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1428..3944
                     /gene="yraJ"
                     /locus_tag="ACHK0Z_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             1428..3944
                     /gene="yraJ"
                     /locus_tag="ACHK0Z_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4500092.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALYL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3955..5046
                     /gene="yraK"
                     /locus_tag="ACHK0Z_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             3955..5046
                     /gene="yraK"
                     /locus_tag="ACHK0Z_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4500093.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5089..5949)
                     /gene="rsmI"
                     /locus_tag="ACHK0Z_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             complement(5089..5949)
                     /gene="rsmI"
                     /locus_tag="ACHK0Z_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU4500094.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6013..8001
                     /gene="lpoA"
                     /locus_tag="ACHK0Z_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             6013..8001
                     /gene="lpoA"
                     /locus_tag="ACHK0Z_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU4500095.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTTQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFR
                     LEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQV
                     QGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7959..8354
                     /gene="yraN"
                     /locus_tag="ACHK0Z_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             7959..8354
                     /gene="yraN"
                     /locus_tag="ACHK0Z_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU4500096.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8374..8964
                     /gene="diaA"
                     /locus_tag="ACHK0Z_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             8374..8964
                     /gene="diaA"
                     /locus_tag="ACHK0Z_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU4500097.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8974..9549
                     /gene="dolP"
                     /locus_tag="ACHK0Z_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             8974..9549
                     /gene="dolP"
                     /locus_tag="ACHK0Z_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4500098.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9663..10703)
                     /gene="yraQ"
                     /locus_tag="ACHK0Z_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             complement(9663..10703)
                     /gene="yraQ"
                     /locus_tag="ACHK0Z_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4500099.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10776..11411)
                     /gene="yraR"
                     /locus_tag="ACHK0Z_RS00055"
                     /old_locus_tag="pgaptmp_000011"
     CDS             complement(10776..11411)
                     /gene="yraR"
                     /locus_tag="ACHK0Z_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4500100.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11539..12057
                     /gene="yhbO"
                     /locus_tag="ACHK0Z_RS00060"
                     /old_locus_tag="pgaptmp_000012"
     CDS             11539..12057
                     /gene="yhbO"
                     /locus_tag="ACHK0Z_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU4500101.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12037..12480)
                     /gene="yhbP"
                     /locus_tag="ACHK0Z_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             complement(12037..12480)
                     /gene="yhbP"
                     /locus_tag="ACHK0Z_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU4500102.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12531..12833
                     /gene="yhbQ"
                     /locus_tag="ACHK0Z_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             12531..12833
                     /gene="yhbQ"
                     /locus_tag="ACHK0Z_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU4500103.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(12820..13323)
                     /gene="yhbS"
                     /locus_tag="ACHK0Z_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             complement(12820..13323)
                     /gene="yhbS"
                     /locus_tag="ACHK0Z_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4500104.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13317..13841)
                     /gene="ubiT"
                     /locus_tag="ACHK0Z_RS00080"
                     /old_locus_tag="pgaptmp_000016"
     CDS             complement(13317..13841)
                     /gene="ubiT"
                     /locus_tag="ACHK0Z_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU4500105.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14050..15045
                     /gene="ubiU"
                     /locus_tag="ACHK0Z_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             14050..15045
                     /gene="ubiU"
                     /locus_tag="ACHK0Z_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU4500106.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15054..15932
                     /gene="ubiV"
                     /locus_tag="ACHK0Z_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             15054..15932
                     /gene="ubiV"
                     /locus_tag="ACHK0Z_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU4500107.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAICSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16138..17145
                     /gene="yhbW"
                     /locus_tag="ACHK0Z_RS00095"
                     /old_locus_tag="pgaptmp_000019"
     CDS             16138..17145
                     /gene="yhbW"
                     /locus_tag="ACHK0Z_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HGU4500108.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGRVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17263..18507)
                     /gene="mtr"
                     /locus_tag="ACHK0Z_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             complement(17263..18507)
                     /gene="mtr"
                     /locus_tag="ACHK0Z_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU4500109.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18661..20550)
                     /gene="deaD"
                     /locus_tag="ACHK0Z_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             complement(18661..20550)
                     /gene="deaD"
                     /locus_tag="ACHK0Z_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU4500110.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20543..20623)
                     /gene="yrbN"
                     /locus_tag="ACHK0Z_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             complement(20543..20623)
                     /gene="yrbN"
                     /locus_tag="ACHK0Z_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU4500111.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20730..21614)
                     /gene="nlpI"
                     /locus_tag="ACHK0Z_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             complement(20730..21614)
                     /gene="nlpI"
                     /locus_tag="ACHK0Z_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU4500112.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21723..23858)
                     /gene="pnp"
                     /locus_tag="ACHK0Z_RS00120"
                     /old_locus_tag="pgaptmp_000024"
     CDS             complement(21723..23858)
                     /gene="pnp"
                     /locus_tag="ACHK0Z_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU4500113.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24105..24374)
                     /gene="rpsO"
                     /locus_tag="ACHK0Z_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             complement(24105..24374)
                     /gene="rpsO"
                     /locus_tag="ACHK0Z_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU4500114.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24523..25467)
                     /gene="truB"
                     /locus_tag="ACHK0Z_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             complement(24523..25467)
                     /gene="truB"
                     /locus_tag="ACHK0Z_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU4500115.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25467..25868)
                     /gene="rbfA"
                     /locus_tag="ACHK0Z_RS00135"
                     /old_locus_tag="pgaptmp_000027"
     CDS             complement(25467..25868)
                     /gene="rbfA"
                     /locus_tag="ACHK0Z_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU4500116.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26032..28704)
                     /gene="infB"
                     /locus_tag="ACHK0Z_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             complement(26032..28704)
                     /gene="infB"
                     /locus_tag="ACHK0Z_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU4500117.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28729..30216)
                     /gene="nusA"
                     /locus_tag="ACHK0Z_RS00145"
                     /old_locus_tag="pgaptmp_000029"
     CDS             complement(28729..30216)
                     /gene="nusA"
                     /locus_tag="ACHK0Z_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU4500118.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQALEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30244..30696)
                     /gene="rimP"
                     /locus_tag="ACHK0Z_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             complement(30244..30696)
                     /gene="rimP"
                     /locus_tag="ACHK0Z_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU4500119.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30903..30979)
                     /locus_tag="ACHK0Z_RS00155"
                     /old_locus_tag="pgaptmp_000031"
     tRNA            complement(30903..30979)
                     /locus_tag="ACHK0Z_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30943..30945),aa:Met,seq:cat)
     gene            31327..32670
                     /gene="argG"
                     /locus_tag="ACHK0Z_RS00160"
                     /old_locus_tag="pgaptmp_000032"
     CDS             31327..32670
                     /gene="argG"
                     /locus_tag="ACHK0Z_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU4500120.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32678..34303)
                     /gene="yhbX"
                     /locus_tag="ACHK0Z_RS00165"
                     /old_locus_tag="pgaptmp_000033"
     CDS             complement(32678..34303)
                     /gene="yhbX"
                     /locus_tag="ACHK0Z_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4500121.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34760..34846)
                     /locus_tag="ACHK0Z_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     tRNA            complement(34760..34846)
                     /locus_tag="ACHK0Z_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34810..34812),aa:Leu,seq:gag)
     gene            complement(34861..35193)
                     /gene="secG"
                     /locus_tag="ACHK0Z_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             complement(34861..35193)
                     /gene="secG"
                     /locus_tag="ACHK0Z_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU4500122.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35421..36758)
                     /gene="glmM"
                     /locus_tag="ACHK0Z_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             complement(35421..36758)
                     /gene="glmM"
                     /locus_tag="ACHK0Z_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU4500123.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36751..37599)
                     /gene="folP"
                     /locus_tag="ACHK0Z_RS00185"
                     /old_locus_tag="pgaptmp_000037"
     CDS             complement(36751..37599)
                     /gene="folP"
                     /locus_tag="ACHK0Z_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU4500124.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVVPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37689..39623)
                     /gene="ftsH"
                     /locus_tag="ACHK0Z_RS00190"
                     /old_locus_tag="pgaptmp_000038"
     CDS             complement(37689..39623)
                     /gene="ftsH"
                     /locus_tag="ACHK0Z_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU4500125.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39723..40352)
                     /gene="rlmE"
                     /locus_tag="ACHK0Z_RS00195"
                     /old_locus_tag="pgaptmp_000039"
     CDS             complement(39723..40352)
                     /gene="rlmE"
                     /locus_tag="ACHK0Z_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU4500126.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40478..40771
                     /gene="yhbY"
                     /locus_tag="ACHK0Z_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             40478..40771
                     /gene="yhbY"
                     /locus_tag="ACHK0Z_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU4500127.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40927..41403)
                     /gene="greA"
                     /locus_tag="ACHK0Z_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             complement(40927..41403)
                     /gene="greA"
                     /locus_tag="ACHK0Z_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU4500128.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41651..43084
                     /gene="dacB"
                     /locus_tag="ACHK0Z_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             41651..43084
                     /gene="dacB"
                     /locus_tag="ACHK0Z_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4500129.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43124..44296)
                     /gene="cgtA"
                     /locus_tag="ACHK0Z_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             complement(43124..44296)
                     /gene="cgtA"
                     /locus_tag="ACHK0Z_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU4500130.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44312..45277)
                     /gene="yhbE"
                     /locus_tag="ACHK0Z_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /pseudo
     CDS             complement(44312..45277)
                     /gene="yhbE"
                     /locus_tag="ACHK0Z_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(45404..45661)
                     /gene="rpmA"
                     /locus_tag="ACHK0Z_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             complement(45404..45661)
                     /gene="rpmA"
                     /locus_tag="ACHK0Z_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU4500131.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45682..45993)
                     /gene="rplU"
                     /locus_tag="ACHK0Z_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             complement(45682..45993)
                     /gene="rplU"
                     /locus_tag="ACHK0Z_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU4500132.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46252..47223
                     /gene="ispB"
                     /locus_tag="ACHK0Z_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             46252..47223
                     /gene="ispB"
                     /locus_tag="ACHK0Z_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU4500133.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVQEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47451..47729
                     /gene="sfsB"
                     /locus_tag="ACHK0Z_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             47451..47729
                     /gene="sfsB"
                     /locus_tag="ACHK0Z_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU4500134.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47777..49036)
                     /gene="murA"
                     /locus_tag="ACHK0Z_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             complement(47777..49036)
                     /gene="murA"
                     /locus_tag="ACHK0Z_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU4500135.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49091..49345)
                     /gene="ibaG"
                     /locus_tag="ACHK0Z_RS00250"
                     /old_locus_tag="pgaptmp_000050"
     CDS             complement(49091..49345)
                     /gene="ibaG"
                     /locus_tag="ACHK0Z_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU4500136.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49505..49798)
                     /gene="mlaB"
                     /locus_tag="ACHK0Z_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             complement(49505..49798)
                     /gene="mlaB"
                     /locus_tag="ACHK0Z_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU4500137.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49798..50433)
                     /gene="mlaC"
                     /locus_tag="ACHK0Z_RS00260"
                     /old_locus_tag="pgaptmp_000052"
     CDS             complement(49798..50433)
                     /gene="mlaC"
                     /locus_tag="ACHK0Z_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU4500138.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50452..51003)
                     /gene="mlaD"
                     /locus_tag="ACHK0Z_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             complement(50452..51003)
                     /gene="mlaD"
                     /locus_tag="ACHK0Z_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU4500139.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51008..51790)
                     /gene="mlaE"
                     /locus_tag="ACHK0Z_RS00270"
                     /old_locus_tag="pgaptmp_000054"
     CDS             complement(51008..51790)
                     /gene="mlaE"
                     /locus_tag="ACHK0Z_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU4500140.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51798..52607)
                     /gene="mlaF"
                     /locus_tag="ACHK0Z_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             complement(51798..52607)
                     /gene="mlaF"
                     /locus_tag="ACHK0Z_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU4500141.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52817..53794
                     /gene="yrbG"
                     /locus_tag="ACHK0Z_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             52817..53794
                     /gene="yrbG"
                     /locus_tag="ACHK0Z_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU4500142.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53808..54794
                     /gene="kdsD"
                     /locus_tag="ACHK0Z_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             53808..54794
                     /gene="kdsD"
                     /locus_tag="ACHK0Z_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU4500143.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELEQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54815..55381
                     /gene="kdsC"
                     /locus_tag="ACHK0Z_RS00290"
                     /old_locus_tag="pgaptmp_000058"
     CDS             54815..55381
                     /gene="kdsC"
                     /locus_tag="ACHK0Z_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU4500144.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55378..55953
                     /gene="lptC"
                     /locus_tag="ACHK0Z_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             55378..55953
                     /gene="lptC"
                     /locus_tag="ACHK0Z_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU4500145.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55922..56479
                     /gene="lptA"
                     /locus_tag="ACHK0Z_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             55922..56479
                     /gene="lptA"
                     /locus_tag="ACHK0Z_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU4500146.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56486..57211
                     /gene="lptB"
                     /locus_tag="ACHK0Z_RS00305"
                     /old_locus_tag="pgaptmp_000061"
     CDS             56486..57211
                     /gene="lptB"
                     /locus_tag="ACHK0Z_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU4500147.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57259..58692
                     /gene="rpoN"
                     /locus_tag="ACHK0Z_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             57259..58692
                     /gene="rpoN"
                     /locus_tag="ACHK0Z_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU4500148.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58715..59002
                     /gene="hpf"
                     /locus_tag="ACHK0Z_RS00315"
                     /old_locus_tag="pgaptmp_000063"
     CDS             58715..59002
                     /gene="hpf"
                     /locus_tag="ACHK0Z_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU4500149.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59120..59611
                     /gene="ptsN"
                     /locus_tag="ACHK0Z_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             59120..59611
                     /gene="ptsN"
                     /locus_tag="ACHK0Z_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU4500150.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLEAPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59657..60511
                     /gene="rapZ"
                     /locus_tag="ACHK0Z_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             59657..60511
                     /gene="rapZ"
                     /locus_tag="ACHK0Z_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU4500151.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60508..60780
                     /gene="npr"
                     /locus_tag="ACHK0Z_RS00330"
                     /old_locus_tag="pgaptmp_000066"
     CDS             60508..60780
                     /gene="npr"
                     /locus_tag="ACHK0Z_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU4500152.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60994..61626
                     /gene="yrbL"
                     /locus_tag="ACHK0Z_RS00335"
                     /old_locus_tag="pgaptmp_000067"
     CDS             60994..61626
                     /gene="yrbL"
                     /locus_tag="ACHK0Z_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU4500153.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61623..62351)
                     /gene="mtgA"
                     /locus_tag="ACHK0Z_RS00340"
                     /old_locus_tag="pgaptmp_000068"
     CDS             complement(61623..62351)
                     /gene="mtgA"
                     /locus_tag="ACHK0Z_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU4500154.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62348..63001)
                     /gene="elbB"
                     /locus_tag="ACHK0Z_RS00345"
                     /old_locus_tag="pgaptmp_000069"
     CDS             complement(62348..63001)
                     /gene="elbB"
                     /locus_tag="ACHK0Z_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU4500155.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63231..65567)
                     /gene="arcB"
                     /locus_tag="ACHK0Z_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             complement(63231..65567)
                     /gene="arcB"
                     /locus_tag="ACHK0Z_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU4500156.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLCFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65663..66592)
                     /gene="yhcC"
                     /locus_tag="ACHK0Z_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             complement(65663..66592)
                     /gene="yhcC"
                     /locus_tag="ACHK0Z_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU4500157.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67267..71727
                     /gene="gltB"
                     /locus_tag="ACHK0Z_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             67267..71727
                     /gene="gltB"
                     /locus_tag="ACHK0Z_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU4500158.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71740..73158
                     /gene="gltD"
                     /locus_tag="ACHK0Z_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     CDS             71740..73158
                     /gene="gltD"
                     /locus_tag="ACHK0Z_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU4500159.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEDNMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73342..74469
                     /gene="yhcG"
                     /locus_tag="ACHK0Z_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             73342..74469
                     /gene="yhcG"
                     /locus_tag="ACHK0Z_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGU4500160.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74529..74993)
                     /gene="nanQ"
                     /locus_tag="ACHK0Z_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             complement(74529..74993)
                     /gene="nanQ"
                     /locus_tag="ACHK0Z_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU4500161.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74990..75865)
                     /gene="nanK"
                     /locus_tag="ACHK0Z_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             complement(74990..75865)
                     /gene="nanK"
                     /locus_tag="ACHK0Z_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4500162.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQWLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75862..76551)
                     /gene="nanE"
                     /locus_tag="ACHK0Z_RS00385"
                     /old_locus_tag="pgaptmp_000077"
     CDS             complement(75862..76551)
                     /gene="nanE"
                     /locus_tag="ACHK0Z_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU4500163.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76599..78089)
                     /gene="nanT"
                     /locus_tag="ACHK0Z_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             complement(76599..78089)
                     /gene="nanT"
                     /locus_tag="ACHK0Z_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU4500164.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78198..79091)
                     /gene="nanA"
                     /locus_tag="ACHK0Z_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             complement(78198..79091)
                     /gene="nanA"
                     /locus_tag="ACHK0Z_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4500165.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79213..80004)
                     /gene="nanR"
                     /locus_tag="ACHK0Z_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             complement(79213..80004)
                     /gene="nanR"
                     /locus_tag="ACHK0Z_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4500166.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            80384..81751
                     /gene="dcuC"
                     /locus_tag="ACHK0Z_RS00405"
                     /old_locus_tag="pgaptmp_000081"
     CDS             80384..81751
                     /gene="dcuC"
                     /locus_tag="ACHK0Z_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4500167.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(81794..82291)
                     /gene="sspB"
                     /locus_tag="ACHK0Z_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             complement(81794..82291)
                     /gene="sspB"
                     /locus_tag="ACHK0Z_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU4500168.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(82297..82935)
                     /gene="sspA"
                     /locus_tag="ACHK0Z_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             complement(82297..82935)
                     /gene="sspA"
                     /locus_tag="ACHK0Z_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU4500169.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(83330..83722)
                     /gene="rpsI"
                     /locus_tag="ACHK0Z_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             complement(83330..83722)
                     /gene="rpsI"
                     /locus_tag="ACHK0Z_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU4500170.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83738..84166)
                     /gene="rplM"
                     /locus_tag="ACHK0Z_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             complement(83738..84166)
                     /gene="rplM"
                     /locus_tag="ACHK0Z_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU4500171.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(84385..85512)
                     /gene="zapE"
                     /locus_tag="ACHK0Z_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             complement(84385..85512)
                     /gene="zapE"
                     /locus_tag="ACHK0Z_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU4500172.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85706..86104
                     /gene="zapG"
                     /locus_tag="ACHK0Z_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             85706..86104
                     /gene="zapG"
                     /locus_tag="ACHK0Z_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU4500173.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            86258..87625
                     /gene="degQ"
                     /locus_tag="ACHK0Z_RS00440"
                     /old_locus_tag="pgaptmp_000088"
     CDS             86258..87625
                     /gene="degQ"
                     /locus_tag="ACHK0Z_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU4500174.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87715..88782
                     /gene="degS"
                     /locus_tag="ACHK0Z_RS00445"
                     /old_locus_tag="pgaptmp_000089"
     CDS             87715..88782
                     /gene="degS"
                     /locus_tag="ACHK0Z_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU4500175.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88844..89782)
                     /gene="mdh"
                     /locus_tag="ACHK0Z_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             complement(88844..89782)
                     /gene="mdh"
                     /locus_tag="ACHK0Z_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4500176.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            90217..90687
                     /gene="argR"
                     /locus_tag="ACHK0Z_RS00455"
                     /old_locus_tag="pgaptmp_000091"
     CDS             90217..90687
                     /gene="argR"
                     /locus_tag="ACHK0Z_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU4500177.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22406 a        24360 c        22533 g        21551 t
ORIGIN      
        1 aatatgatta tgtacttgtt acaaggataa ggttataaat gaataaagtt acaaaaacag
       61 ctattgtggg cttacttgcg cttttcgcag gtaatgccgc tgcaaccgat ggtgaaatag
      121 tttttgatgg cgagatttta aaatccgctt gtgaaatcaa tgactctgat aagaaaattg
      181 aagttgctct tggtcactat aatgccgaac agtttcgttc tgttggcgat cgtagcccaa
      241 aaattccatt cactattcct ttagttaact gcccagtgac tgggtgggag cacgataacg
      301 gcaacgttga agcgtctttc cgtttatggc tggaaacgcg ggataatggc accgtcccta
      361 acttccctaa tctggcaaaa gtgggctctt ttgctggcac agcggcaaca ggtgtgggta
      421 tccgtattga cgatgcggaa agcggaaacc ttatgccact gaatgctatg gggaatgata
      481 acacggttta tcagatccca gcggactcca atggtattgt caatgttgac ctcatcgctt
      541 actacgtatc aaccgttgaa gcatcagaaa tcaccccagg ggaagcagac gctgtcgtta
      601 acgtaacgct ggattaccgt taatacgtta cggcgttatc tgacctttca gataacgccc
      661 ttttccttcc tctttctcgt tgtatcaggt tgaaaaataa ctatgtcaaa acgaacattc
      721 gcggtgataa taaccttgtt gtgtagcttc tgtatcggcc aggcgcttgc agggggtatc
      781 gttttacagc gaacgcgagt gatctatgat gccagccgca aagaggctgc gttacctgtc
      841 gcaaacaaag gcgcagaaac gccttattta ctgcaatcat gggtagataa tatagatggt
      901 acaagccgtg ccccatttat tataacccca ccgctatttc gtcttgaagc tggcgatgac
      961 tcatcactgc gaattattaa aacagctgat aacctgcctg aaaataaaga gtcgctgttc
     1021 tacattaatg ttcgtgccat tccagcaaag aaaaaatcag atgatgttaa tgctaacgag
     1081 ttgacgctgg tatttaaaac acggatcaaa atgttttatc gccccgcaca cctgaaggga
     1141 cgggtaaacg atgcgtggac atcactggaa tttaaacgta gtgaccattc actcaatata
     1201 tataacccaa ctgaatatta cgtcgtattt gccggactgg cagtcgataa aaccgatctc
     1261 acaagcaaaa ttgaatatat cgcgcccgga gaacataaac agttaccact tcctgcatct
     1321 ggcggaaaga acgtgaaatg ggctgcaatc aatgattatg gcggcagttc cgggacagaa
     1381 actcgtccac tgcaataaca aatataaaaa acacaggtca tcagggaatg ccacaacgac
     1441 accaccaggg acataaacgc acaccgaaac agttggcgct catcatcaaa cgctgtttgc
     1501 cgatggtgct cactggcagc ggcatgcttt gcactaccgc taacgccgaa gagtattatt
     1561 tcgaccccat tatgctggaa actacaaaaa gtggtatgca aacaactgat ctgtcacgtt
     1621 tttcaaaaaa atacgcacaa ctaccaggaa cttatcaggt tgatatctgg ctgaataaaa
     1681 agaaggtttc acagaaaaaa attacattta ccgccaatgc agagcaactt ctgcagccac
     1741 agtttacggt agaacaacta cgtgagctgg gtattaaggt ggatgaaatc ccggcgctgg
     1801 ctgaaaaaga tgacgatagc gtgatcaact cgcttgaaca aatcattccc ggtacagctg
     1861 ctgaatttga tttcaatcat cagcgactta atttgagcat tccccaaatt gcactgtacc
     1921 gtgatgcaag aggttacgtc tccccttctc gttgggacga tggtatacca acgctgttta
     1981 ccaactactc gtttacaggt tctgataacc gttaccgcca gggcaatcgt agccaacgac
     2041 agtacctgaa tatgcaaaat ggtgctaatt ttggcccctg gcgattacgt aactattcta
     2101 cgtggacacg caacgatcag gcgtcaagct ggaacactat cagtagttat ttacaacgtg
     2161 atatcaaggc gttgaagtct cagttgcttc tgggagaaag cgccaccagc ggcagtattt
     2221 tttccagcta cacctttact ggcgtgcaac tcgcttccga cgataatatg ttgccaaaca
     2281 gccagcgcgg atttgcccca acggtacgcg gtatcgcaaa cagtagtgca atcgtgacta
     2341 tcaggcaaaa tggttatgtg atctatcaaa gcaacgtgcc agcgggtgcc tttgaaatta
     2401 acgatctcta cccctcttcc aacagcggcg atttagaagt cacgattgaa gaaagtgacg
     2461 gtacgcaacg tcgctttatc cagccttatt cttcattacc catgatgcag cgacctgggc
     2521 atctaaaata tagcgcgacc gctggacgct atcgcgctga tgcaaacagt gatagcaagg
     2581 aacccgaatt tgctgaagcc acggcaatat atggtttgaa taatactttt acgctgtatg
     2641 gcggcctgct cggttctgaa gattattatg cgctggggat cggtatcggc ggcacacttg
     2701 gcgcactggg cgcgttgtcg atggatatca acagagctga cacccaattc gataaccagc
     2761 actcttttca tggctatcaa tggcgtacgc agtacatcaa agatatcccg gaaaccaaca
     2821 ccaatatcgc tgtcagctac tatcgctata ccaacgatgg ctattttagt tttaatgaag
     2881 ccaatacccg taattggaac tataacagtc gccaaaaaag tgaaattcaa ttcaacatca
     2941 gccagacaat atttgatggg gtaagtctgt atgcctccgg ttcgcagcaa gactattggg
     3001 gcaataacga gaaaaacagg aatatctctg ttggggtttc cggtcagcaa tggggaattg
     3061 gttacagcct gaattatcaa tacagtcgct acacggatca aaataatgac cgcgcactct
     3121 atttgaatct cagtattccg ttagaacgct ggttaccgcg tagccgggtt tcctatcaga
     3181 tgaccagcca gaaagatcgc ccaacccaac atgaaatgcg tcttgatggc tcactgctgg
     3241 atgatggtcg cctgagctat agcctggaac aaagtctgga tgacgataac aaccataaca
     3301 gtagcctgaa cgccagttac cgttcacctt atggcacctt cagtgccgga tacagctacg
     3361 gtaatgacag cagccaatac aattacggcg ttaccggcgg cgtggttatc catcctcatg
     3421 gcgtgacgct ctcgcaatat ctgggcaacg cttttgcgct tatcgatgct aatggggctt
     3481 ctggcgtgag gatacaaaac tatccgggga ttgctactga tccctttggc tatgcagtgg
     3541 ttccttatct cacgacttac caggaaaacc gtctctcggt agatactacg cagctgcccg
     3601 ataacgtcga tcttgagcaa acaacacagt ttgtggtgcc caacagaggt gcaatggtag
     3661 cggcgcgttt caacgccaat atcggttatc gcgtacttgt tacagtcagc gatcgcaacg
     3721 gtaaaccgtt gccctttggc gctcttgcca gcaacgatga gacggggcaa caaagtatcg
     3781 tcgatgaggg cggcatacta tatctctctg ggatatcgag taaatcacaa agctggactg
     3841 tacgctgggg aaatcaggca gatcaacaat gtcagtttgc ttttagtaca ccggattcag
     3901 aaccaacaac ctctgtatta caaggcacgg cgcagtgcca ttaaggataa aaaaatgaaa
     3961 agagcgcctc ttataacagg acttttgttg atatccacat cctgcgctta tgcctcctca
     4021 ggagggtgtg gagctgacag cactagcggt gcgacaaatt acagcagtgt ggttgatgat
     4081 gttacggtga accagacaga taacgtgaca ggacgggagt ttacctctgc cacgctaagt
     4141 agcactaact ggcaatacgc ctgtacctgc tctgcgggta aggcagttaa acttgtctat
     4201 atggtcagcc ctgtacttac caccactgga catcaagcag gatattacaa actcaatgat
     4261 agtctggata ttaaaaccac attacaggcg aatgacattc caggactcgt gactgaccag
     4321 accgtttctg ttaacacccg attcacacag ataaaaagca acaccgtata ttctgctgca
     4381 acccaaacgg gtgtttgcca gggtgacacg tctcgttatg gacccgttaa tattggtgca
     4441 aataccacct ttaccctgta tgtcaccaag ccatttctcg gctcgatgac cattccgaaa
     4501 acggatattg ccgtcattaa aggcgcatgg gtcgatggaa tgggaagccc gtctacaggt
     4561 gacttccatg atttagtcaa gttatcgatt cagggaaatc tcaccgcccc acagtcgtgc
     4621 aaaattaatc agggcgatgt tattaaggtt aattttggat tcatcaatgg tcagaagttt
     4681 accacccgca atgccatgcc agatggtttt actccagtag actttgatat cacttatgac
     4741 tgtggtgata cttcaaagat taaaaactcg ttgcaaatgc gcatcgacgg tacaactggg
     4801 gtagtagacc agtacaacct ggtcgccaga cgaagaagtt cagacaatgc gcccgatgtc
     4861 ggtattcgta ttgaaaatct cggcggcgga gttgcaaata ttccttttca gaacggtatc
     4921 cttcccgttg atccttccgg gcatggcacc atcaacatgc gcgcctggcc agttaatctg
     4981 gtcggtggtg agctggaaac aggaaaattt cagggcaccg ccaccattac cgtcatcgtg
     5041 cggtaaaaga agacgttgcg ttttgattaa cgcaacgctt cggcactctt acccctgctg
     5101 ctccagcgca tacttataca gcgcattttt cttcacgccg tgaatttctg ccgccagcgc
     5161 cgccgctttt ttcaacggca gttctgcctg tagcagcgcc agcgtgcgca gggcatcggc
     5221 gggtaagtct tcttcctgtg ctttatgacc ttcgataatc agcaccattt cgcctttgcg
     5281 acggttttca tcttctttta cccacgccag cagctcgcca acgggcgcgc cgtgaatggt
     5341 ttcccaggtt ttggtcagct cacgcgccag aaccacgtag cgggattcgc ctaataccgc
     5401 aacgatatct tccaggctat ctaacagacg gtgggtggat tcataaaaaa tcagcgtgcg
     5461 cggctccgct tcaatggctt ttagcgcatc acggcggcct tttgatttgg caggtaaaaa
     5521 gccttcgtaa cagaaacggt cagagggtaa acccgcggcg cttaacgcag tgatagcagc
     5581 acacggcccg ggtagcggca ccacgcaaat ccccgcttca cggcaggtac gcaccagatg
     5641 gtagccagga tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa tgttctgccc
     5701 ttcttgcagc ttcgccagca gcgtttcggc tttttgttgt tcgttatggt cgtgcagcgc
     5761 aaacaaccgg gcattaatcc caaaatgttg cagcaataaa ccggtgtgac gagtatcctc
     5821 ggcggcaatc agatcaacgg cctgtaatac ctctaacgca cgctgggtga tatccgccag
     5881 attgccgatt ggcgtcggta caatgtaaag ctggccctga gaattatccg ccgattggtg
     5941 ttgtttcatt gtgtcgtccg tattgccgat ttaatattga gcattgcgta aaaaaatatc
     6001 actggataca ttatggtacc ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct
     6061 gttgttctgg cagccctgat tttcgccggt tgtggcaccc atactcccga tcagtccact
     6121 gcttatatgc agggcacgac gcaggctgat tctgcctttt atcttcagca gatgcagcaa
     6181 agctctgatg ataccaggat caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa
     6241 ggtaaaaccg ggcaggcggt tgagttgttt aaccaactac cgcaagaact gaacgattct
     6301 cagcgtcgcg agaaaacact gctggcggta gagattaaac tggcgcagaa agattttgct
     6361 ggcgcgcaaa acttgctggc gaaaatcaca cctgccgatt tagaacaaaa ccagcaagcg
     6421 cgttactggc aggcaaaaat cgatgccagc caggggcgtc cttccattga tttactgcgc
     6481 gcgttaattg ctcaggaacc gctgctcggc gcgaaagaaa aacagcagaa tattgatgcc
     6541 acctggcagg cgctctcctc catgactcag gaacaggcga atacgctggt gatcaacgcc
     6601 gacgaaaata ttctgcaagg ctggctggat ctgcagcgcg tctggtttga taaccgtaac
     6661 gatcccgaca tgatgaaagc cgggatcgcc gactggcaaa aacgttatcc gaacaatccg
     6721 ggcgcgaaaa tgctgccaac gcagttggtt aacgtaaaag cgtttaaacc agcctcgacc
     6781 aacaaaatcg ccctgctgtt gccgctgaat ggccaggcag cggtatttgg tcgcactatt
     6841 cagcaaggct ttgaagcggc gaaaaatatc ggcactcagc cagtggcggc tcaggtagct
     6901 gccgcacctg ccgcagacgt agctgaacaa cctcagccgc aaaccgtgga tggcgttgcc
     6961 agcccggcac aagcctcggt tagcgatctg accggtgaac agcctgcggc acagccagcg
     7021 cctgtaagca ccccggcagc aaccaccaca gcggtaagcg cgccagcgaa tccatccgca
     7081 gaactgaaaa tctacgatac ctcatcacaa ccacttagcc agatcttaag ccaggttcag
     7141 caggatggcg cgagtattgt ggttggtccg ttgctgaaaa ataacgttga agagttgctt
     7201 aagagcaaca ccccgctgaa cgtgctggca ctgaaccagc cggagaatat cgaaaaccgc
     7261 gtcaatattt gttacttcgc gctttcaccg gaagacgaag cgcgcgatgc agcgcgtcat
     7321 attcgtgacc agggtaaaca agcgccgctg gtgctgatcc cacgcagttc attgggcgat
     7381 cgcgtagcca atgcgtttgc gcaggagtgg cagaaactgg gcggcggcac cgttctgcaa
     7441 caaaaatttg gttccaccag cgaattacgc gcgggtgtta acggcggttc tggtatcgct
     7501 ttaacgggta ccccgattac tcccagagcg acaaccgatg accagttcac caataatggc
     7561 ggtcgtgtcg atgcggtgta cattgtggca acgccgggtg aaatcgcttt tatcaaaccg
     7621 atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc tgtacgccag ctcccgcagt
     7681 gcgcaaggga ccgctggccc ggatttccga ctggagatgg aaggcttgca gtacagcgaa
     7741 atcccgatgc tggcaggcgg taatctaccg ttaatgcagc aggcactcag cgcggtgaat
     7801 aacgattatt cactggctcg catgtatgcg atgggcgtcg atgcctggtc gctggcaaat
     7861 catttctcac aaatgcgcca ggttcagggt tttgaaatca acggtaatac cggaagcctg
     7921 acggcaaacc cggattgcgt gattaacagg aagttatcat ggctacagta ccaacaaggt
     7981 caggtagtcc ccgccagtta accaccaaac agaccggcga tgcgtgggaa gtacaagcgc
     8041 gtcgctggct ggaaggcaaa ggactgcgat ttgtcgccgc taacgtgaac gagcgtggcg
     8101 gcgagatcga tctgataatg cgtgaaggcc ggaccaccgt ttttgtcgag gtacgctatc
     8161 gccgctctgc gctttatggc ggcgcggcag ccagtgtgac ccgcagcaaa caacacaaat
     8221 tattacagac tgcccgcttg tggctcgcgc gtcataatgg gagttttgat actgtggatt
     8281 gccggttcga tgtggtagcc ttcaccggga atgaggttga gtggattaag gatgccttta
     8341 atgaccactc ataattaagg tttaaggatt agcgtgcaag aaagaattaa agcttgcttc
     8401 actgaaagca ttcaaactca aattgcggcg gcagaggcgc ttccggatgc catctcccgt
     8461 gcagccatga cgctggttca gtctctgctc aatggcaaca aaatcctctg ttgtggtaat
     8521 ggaacttccg ctgccaatgc acagcatttt gctgccagca tgatcaaccg tttcgaaacg
     8581 gagcggccca gcttacctgc cattgcacta aatactgata atgttgtctt aacggcgatt
     8641 gccaacgatc gcttacatga tgaagtgtat gcaaaacagg tgcgggcgct gggtcatgcg
     8701 ggagatgtat tgttagccat ttccacccgt ggcaacagcc gcgatattgt aaaagcggta
     8761 gaagctgccg ttacgcgtga tatgactatt gtggcattga ccggctatga cggcggcgaa
     8821 cttgcaggtt tgttagggcc acaagatgtg gagatccgca ttccttcgca tcgtagtgct
     8881 cgcattcagg aaatgcatat gctgacggta aattgcctgt gcgatctgat cgataacacg
     8941 cttttccctc accaggatgt ttaaggagaa tacatgaagg cattatcgcc aatcgcagtc
     9001 cttatttccg cgctgctgtt gcaaggttgt gttgccgctg ccgtagtggg taccgctgct
     9061 gtgggtacca aagccgcaac tgacccacgc agtgtcggca cccaggtgga cgatggtacc
     9121 ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac agattaagaa agaagcccgt
     9181 attaatgtca cggcgtatca gggcaaagtg ctgctggttg ggcagtcacc aaacgctgaa
     9241 ctttcggctc gcgccaaaca gattgctatg ggcgtagacg gtgccaacga agtgtataac
     9301 gagattcgtc agggccagcc gattggtctg ggcgaagcgt ctaacgatac gtggatcacc
     9361 accaaagtgc gttcgcagct cttaaccagc gacctggtga aatcgtccaa cgtgaaagtg
     9421 accaccgaaa acggtgaagt gttcctgatg gggctggtga ctgaacgtga agcgaaagcg
     9481 gcggcagata ttgccagccg ggtgagcggc gtaaagcggg taaccacagc gtttacgttt
     9541 attaaatagc agccccttgt aatgcctgat gcgacgcttg ccgcgtctta tcaggcctac
     9601 aacgatgcaa actgtaggtc ggataaggcg tttacgccgc atccgacatg ttttccctca
     9661 aatcagaaca acagcgccag cccgccgaca atcacaccag acactgccac catcgcccct
     9721 gtcagccata aggctttcgc tgggaacgct ttgcgcagca taatcagtga cggcaaactc
     9781 accgccggga gcgtcatcaa caatgccagc gccggagcag ttcccatacc tgccagcatc
     9841 atcgtttgta caatcggaat ttctgctgcc gtgggaatga caaacaagca tcctgctacc
     9901 gccatcgcca tcacccacat caggctgtta tcgacagcac catcggcatg ggggaataac
     9961 cagacgcgag cggcacccaa caccagtact gcaaggatgt aaaccgggat cgtactccag
    10021 aaaagcgtcc atagcgccct gccccagcgg ctaaaaaaac cgccctgtgc ttccggtatg
    10081 tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca cccatttttg caccagcgtc
    10141 gcaatcagca acaccatcac cagcccggcc accagacgaa tcgccgcaaa accccagccg
    10201 aggacaaacc ccataaacac cagcgtcgcc gggtttaaca ccggattgcc catccagaat
    10261 gccagcgcac cgcccatcga cacctgttgg cgacgcattc ccgccgcgac cggagccgca
    10321 cagcaggtac acatcatgcc cggcagcgaa aacagcgttc ccagcagcgt gccgcgaaag
    10381 cgcgattgcc caagcgtacg caacaaccag tcacgcggga tcagcacctg aatcaacgaa
    10441 ccgaggatca cccccagcac tgccgctttc cataccgcga ggaaatagat catcgcgtaa
    10501 tccaacgccg cctgccacgg attagcatcc gcctgcgcaa ggatagattt accgatactg
    10561 tgggtttcgg cagcagtaaa cgctttgccg tagtaaggtt gccatttcac ataccagaga
    10621 ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc actgaatggg cgttgccgcc
    10681 tgagatgaag actgaccagt catagcattc cccaggaata agttatgata attgagcgcg
    10741 tgaatattac gctcactatc aattcttggg aataattatt cagctctttt gcgtaattct
    10801 gaagagctta aaatcgtcac accttcatgc ccgggccgca tcgcctctgc cagcatgacc
    10861 cgcgccacgt ctctcgcatc aatggatttc cagttaccgg gtaataaacg gaacagcggc
    10921 gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc ccagtaacat cgacgggcga
    10981 gcaatggtca atttcggcca gttctgggcg attaatgctt cttccatctc ccctttgacg
    11041 cggttataga aaaacggcga gtgggcattg gcacccatag cactgaccac cagcatatgc
    11101 tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta ccagcgtgta atcggcatga
    11161 ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc cgagacaaca aaacacaatg
    11221 tcgatgggat cggtgacctg cgccagcgcg tcagtcagtt gcggatcatg gggattaaac
    11281 accccgggca tatcgcccaa cggacgtcgc gtcggcgcgg ctatggcgtt aactttcggt
    11341 tcgttaatca acatccgcag caggtgaccg cccaccagcc ccgttgcgcc tgtaatcagt
    11401 acctgactca tcttcgctcc tttacagaat tgtctgcctt gcgctccacg gctcatgcac
    11461 caggcttaat agaccatgag gtaattatcc ccgattgtgg gaaattcgcc tcatccaatg
    11521 caacaacgcg gaggaagcat gagtaagaaa attgccgttt taatcactga tgaatttgaa
    11581 gattcagaat ttacttcacc cgcagacgag ttccgtaaag ccggacacga agtgattacc
    11641 attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag gagaagccag cgtgaccatc
    11701 gataaatcca tcgatgaagt gacgccagcg gagtttgatg ccctgctgct accgggcggc
    11761 cattcaccgg attatctgcg tggcgacaac cgttttgtca cctttacccg tgattttgtg
    11821 aatagcggca aacctgtttt cgccatctgt cacggtccgc agctgctgat cagcgccgat
    11881 gtgattcggg ggcgcaaact gaccgcggtt aaaccgatca ttattgatgt taaaaacgcg
    11941 ggcgcggaat tttacgatca ggaagtcgtg gtcgataaag atcagctggt taccagccgg
    12001 acaccggacg atctgccagc gtttaaccgc gaggcgttac gcctgctcgg tgcctgagcc
    12061 gcgtagccaa atcatttttt taccaaagcc cagcgtgttg tcggtgaatt tgatttcatc
    12121 gagccggatt tcccacaccg gggccgacag cattctggca accggaaagc gacgattgta
    12181 cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg cggatctcac ctttaaactg
    12241 cacgccgcga attaacgcta ccgttttcgg ctgaccgttt accgttccgg caaccgcagc
    12301 ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc gtcaaaatgt agaaggcgac
    12361 tttctgcgca tcaaacagat aaaaagcatt agcgcaccag agttcccctt cctgctgcac
    12421 acaccaggtg acaacatgtt gttttgccaa ccaacggctg atggtgatga gtgtttccat
    12481 tgctgttctc ttttatactg tgggcctgac cttaacatgc gacgcagacg atgacaccct
    12541 ggtttcttta cttgatccgt accgccgaca ataagcttta taccgggatc accacggatg
    12601 tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc gaaagcactg cgcgggaaag
    12661 gagaactgac gctggcgttt tctgcgccag tgggcgatcg ttcgctggcg ttacgggcgg
    12721 aatatcgcgt taagcaactg accaaacggc aaaaagagcg tctggtagcg gaaagcgcag
    12781 ggtttgcaga gctgttaagc agtctgcaaa ccccggagat taaaagcgat tgaaatgctc
    12841 gtgatactca accagaccag ttacgccatt caacgcgtca tccgctaaac gatgtacctg
    12901 gaaggcgctt tcagtgcctg gccagcggca acgcaggtcg tgatgtgccg ccagttcaaa
    12961 gccaaaacgg ctgtacagcg ccggatcgcc cagcgtcacc actgcggcat agccgaactc
    13021 attaagcgaa tcgagtcctt catagaccag ttggcgtgcc agcccctgtc cacggtattt
    13081 ttcatcaacc gccaacggtg ccatgccgac ccattgcagg tcttcgccct gcacatcaac
    13141 cggactaaat gccacatagc caatgacctg accttcgtca tctgtcgcca ccagccccag
    13201 cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc gcttccgcat cactttcgaa
    13261 tgagcgacgt agcagggcat caatacccgg cgcatcaatg ggaatttcta ctcgaattag
    13321 catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg ttttcattcc cgcctcaaca
    13381 aaatccgcca gttgcagcag catcatgcgt aaggctttcg gcatttgctc cagttcaatg
    13441 gcgtccatca ggtttttcac atacagcccc agctccgtat cgccttcaat caccagccga
    13501 cgctggaaga agagcgtatc cggatcttgt ttacgcgccg caatcatcag cagatcgctg
    13561 gcgtcggcac taaaactcac atcagcttgc gcgttctggc tgaccaccag tttgccattc
    13621 accaccgagg taaaccattg caggtcaata tcacgcacat gaatacttaa ccagcggcct
    13681 tcaagaaact ccagctcgcc atcatccagc gcctgacgga attgccagct taagacctgc
    13741 tcaagaacct ggcgttttag cgcaaatggc gtcagtttta ccggtacact caacagagat
    13801 ggccccaaat gcacaatacg ggaacgcagt ttatccaaca cgagttttac tccctgtttc
    13861 aacaatcatc ctattttgcc atatcagaaa aataacatag cggtataaat caacaattcc
    13921 atatgaaatt gctgctacca ccaatacaac tttaactgcc ttaaatcaaa aattgtcgca
    13981 gcaaggttaa ctaaaatccc agttcgttaa catttttgcg ttttgatagc gcaaccttca
    14041 ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc cggcgcttaa ggcggccatc
    14101 gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg ataccaatgc ccgtcacttc
    14161 gccggcctta actttaccga gaaaaaattg caggaagcgg tgagttttgt ccatcaacat
    14221 cgccgcaaac ttcacatcgc gattaacact tttgcgcatc cggacggtta cgcccgttgg
    14281 cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg cgctgatcct cgccgacctc
    14341 gccatgctag agtacgccgc cgagcgttac ccgcatattg agcgtcatgt gtcagtgcag
    14401 gcttcggcga ccaatgaaga ggcgattaac ttttatcatc gccattttga cgttgctcgc
    14461 gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac aactggcacg ggtcacacct
    14521 gtaccactgg aagtctttgc tttcggcagc ctgtgcatta tgtcggaagg tcgttgctat
    14581 ctgtcgtcgt atctgacggg tgagtcgccc aacaccgtag gcgcgtgttc tccggcccgt
    14641 ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc gcctgaacga agtgctgatc
    14701 gaccgttatc aggacggcga aaacgcaggt tatccgacgc tatgtaaagg gcgttatctg
    14761 gtggacggcg agcgctatca cgcgctggaa gaaccaacca gtctcaatac cctggaactg
    14821 ctgccggagt taatggcggc gaatattgct tcggtgaaaa ttgaaggccg ccagcgtagc
    14881 ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg ctatcgaccg ttgtaaggcc
    14941 gatccgcaaa acttcatacc gcaaagcgcg tggatggaga cgctcgggtc gatgtccgaa
    15001 ggcacgcaga ccacccttgg cgcgtatcac cgtaaatggc agtgagaaga gcaatgaaat
    15061 attccttagg gccagtgctg tggtactggc caaaagagac gctggaagat ttttatcagc
    15121 aggccgccac cagcagcgcc gacgtgattt atcttggtga agcgatatgc agcaagcgcc
    15181 gggcaaccaa agttggcgac tggctggaga tggcaaaatc gctcgccgga agtggcaagc
    15241 agattgtgct ctccacgctg gcgctggtgc aggcatcctc tgaactgggc gaactgaaac
    15301 gctatgttga gaacggtgag tttctgattg aagccagcga tctcggcgtg gtgaatatgt
    15361 gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct gaactgctac aacgcggtga
    15421 cgctgaaaat attgctcaaa cagggcatga tgcgctggtg tatgccggtg gagctttccc
    15481 gcgactggct ggtgaatctg cttaatcagt gcgatgagct gggcattcgc aaccagtttg
    15541 aagtggaagt cctgagctac ggtcatctac cgctggccta ctccgcccgc tgctttaccg
    15601 cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg ctgcattaag tatccgaacg
    15661 ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt actcaatggc attcagacca
    15721 tgagcggcta cgtttacaac ctcggtaacg agctggcatc catgcagggg ctggttgatg
    15781 tggtccgcct gtcaccgcag ggtactgaca ctttcgcgat gctcgacgcc ttccgcgcta
    15841 atgaaaatgg cgctgcgcca ctaccgttga cggcaaacag cgactgtaac ggctactggc
    15901 ggcggctggc cgggctggag ctgcaagcct aagtaaatat gctcgtcata cttcaagttg
    15961 catgtgctgc atctgcattc gttcacccca gtcacttact tatgtaagct cctggggatt
    16021 cactctcttg tcgccttcct gcaactcgaa ttatttagag cacagctcac tttgttaaca
    16081 actttaacta ctctttaatg cagtattaaa gattaatcgg taacaaagtg agctgttatg
    16141 actgataaaa ccattgcgtt ttcgctactc gatctggccc ccattcccga aggttcttca
    16201 gcgcgagaag cattctccca ctctctcgat ctcgcccgtc tggctgaaaa gcgcggctat
    16261 catcgctact ggctggcaga acaccacaat atgactggca ttgccagtgc tgccacgtcg
    16321 gtattgattg gctatctggc ggcgaatacc accacgctgc atctggggtc tggccgcgtg
    16381 atgttgccta accactcacc gttggtcatt gccgaacagt tcggcacgct caatacactc
    16441 tatccggggc gaatcgattt ggggctgggt cgtgcgccag gtagtgacca acggacaatg
    16501 atggcgctgc gtcgccatat gagcggcgat attgataatt tcccccgcga tgtcgcggag
    16561 ctggtggact ggtttgacgc ccgcgatccc aatccgcatg tgcgcccggt accaggctat
    16621 ggcgagaaaa tccccgtgtg gttgttaggc tccagccttt acagcgcgca actggcggcg
    16681 cagcttggtc tgccgttcgc gtttgcctca cacttcgcgc cggatatgtt gttccaggcg
    16741 ctgcatcttt atcgcagcaa cttcaaaccg tcggcacgac tggaaaaacc atacgcgatg
    16801 gtgtgcatca atattatcgc cgccgatagc aaccgcgacg ctgagtttct gtttacctca
    16861 atgcagcaag cctttgtgaa gttgcgccgt ggcgaaaccg ggcaactgcc gccaccgatt
    16921 caaaatatgg atcagttctg gtcgccgtcc gaacagtatg gtgtgcagca ggcgctgagt
    16981 atgtcgttgg taggtgataa agcgaaagtg cgtcatggct tgcagtcgat cctgcgcgaa
    17041 accgacgccg atgagattat ggtcaacggg cagattttcg accaccaggc gcggctgcat
    17101 tcgtttgagc tggcgatgga tgttaaggaa gagttgttgg gatagtgtgt cttaacgcgg
    17161 gaagccttat ccgagctggc aacgctgtcc tacgtagacc tgataagcga agcgcatcag
    17221 gcattgtgta ggcagcagaa atgtcggata aggcaccgct gattactgat acaccggcag
    17281 taaattaaag ctcgataaaa tatggaccag tgcgttgcca acgccaaaca ccagaatcag
    17341 cgtaatcatc ggcttgccgc cccagacgcg gaatttcggg ctgccaaagc gtttacgcga
    17401 tgcgcgggct aacagcgccg gaacaattgc cgcccagatg gtagctgcta aaccagcata
    17461 accaatggcg tacaggaatc cgttcgggaa cagtagacca cccacaacag gcggggcaaa
    17521 ggtcagcaat gccgttttca agcgccccag ggctgagtca tcgaaaccaa acagatctgc
    17581 cagatagtca aacaaaccca gcgttacgcc aaggaacgaa ctcgctaccg caaagtttga
    17641 gaacacgacc agcagaaggt ccaggctacg gctgttcagc acgccgctta atgcctgtac
    17701 cagcacatca atattaccgc ccttctctgc aataccgata aactccggac gcgggatgtt
    17761 acccatcgtc gccagcaacc agatggtata cagcgccagt gccatcagcg taccgtacac
    17821 caggcatttc acgatggttt tcggatcttt gccgtaatac ttcatcaggc ttggcacgtt
    17881 accgtgataa ccaaacgacg ccaggcagaa cggcaatgtc atcagcagat acggcgcata
    17941 agatgcattg ctttctgcaa cgttgaacaa tgtcgcaggc tgcacatgcc ccaacaggct
    18001 gccaaaggtg aggaagaagg taatgacttt cgcccccagc acaatcgccg tcatgcggct
    18061 gacggcctta gtactcaacc acaccacaaa cgctaccagc agtgcaaaac caaaacccgc
    18121 cgcccgtgcc gggacgttta gcgacatctc tgcgaaggtg tgatgcagaa ttgaaccact
    18181 ggcagaaata taggcatagg tcaggatata gagcacaaag gcaatggaaa taccgttgac
    18241 cacgttccag cctttgccca gcaaatcttt ggtgatggtg tcaaaactcg aaccgattcg
    18301 gtaattcagg ttagcttcca gaatcatcaa gccggaatgc agcatacaga accaggtaaa
    18361 gatcagcgcc gccattgacc agaaaaacca cgccccggac atgaccactg gcagagaaaa
    18421 catccctgcg ccaataatgg tgccgccgat aatcaccacg ccgccaagca gcgacggtga
    18481 cgtttgggtg gtggttagtg ttgccatgag ggcttctctc cagtgaaaaa tagtgcgact
    18541 gcgttgttat gcattgcact gtaccagtac acgagtacaa aagacagaaa aaaagccccg
    18601 atggtaaaaa tcggggctgt atatattatt ttacagattg tgttcgctgt tcagcgatga
    18661 ttacgcatca ccaccgaaac gacgacgacc ggtagagtca tcacgacgcg gagcgcggcc
    18721 ttcacgacgt tcgccgctaa aacgacgacc atcaccacgg ccaccttcac ggcgttcacc
    18781 actgaagtta cgaccgcctt cacgacgttc gccaccgaaa ccacgaccac cgccacgacg
    18841 ctcaccgcca gtatgcggct gtgcatcgcc cagtaactgc atgttcatcg gcttgttgag
    18901 aatgcgagta cgcgtaaagt gttgcagcac ttcacccggc atacctttcg gcagttcgat
    18961 ggtggagtga gaagcaaaca gcttgatgtt accaatgtaa cggctgctga tgtcgccttc
    19021 gttagcaatc gcaccaacga tatgacgaac ttcaacacca tcatcgcggc ccacttcaat
    19081 gcggtacagc tgcatatcgc caacatcacg acgttcacga cgcggacgat cttcacggtc
    19141 accacgcggg ccacggtcgt tacgatcgcg cggaccacgg tcatcacggt cacggaattc
    19201 acgtttcgga cgcatcggcg catctggcgg tacgatcaga gtacgttcac cctgtgccat
    19261 tttcagcagt gccgcagcca gagtttcgag atccagctct tcaccttctg cagtcggctg
    19321 aattttgctc agcagtgcgc ggtattgatc cagatcgctg ctttccagct gctgctgtac
    19381 tttagcggcg aatttttcca gacggcgttt gcccagcagt tctgcgttcg gcagttctac
    19441 ttccggaata gtcagcttca tagtacgttc aatgttgcgc agcagacgac gctcgcggtt
    19501 ctcaacgaac agcagcgcgc ggccagcacg acccgcacga ccggtacgac cgatacggtg
    19561 aacgtaagac tcagaatcca tcgggatatc gtagttaact accaggctga tacgctcaac
    19621 gtccaggcca cgggctgcaa cgtcggtcgc aatcaggatg tccagacgac catctttcag
    19681 gcgttccagt gtctgttcac gaagcgcctg gttcatgtca ccgttcagcg cggcgctgtt
    19741 gtagccgtta cgctcaagag cttcagccac ttccagagtc gcgtttttgg tacgaacgaa
    19801 gataatcgcc gcatcaaaat cttccgcttc caggaaacgt accagtgctt cgtttttgcg
    19861 cataccccag acagtccagt agctctggct gatgtcagga cgggtagtca cgctggactg
    19921 aatgcgcact tcctgcggct ctttcataaa gcggcgggta atgcgacgaa tcgcttccgg
    19981 catggttgca gagaacagag cggtctgatg accttccggg atctgcgcca taatggtttc
    20041 aacgtcttcg atgaagccca tgcgcagcat ttcgtcagct tcatccagaa ccagaccgct
    20101 cagtttagag aggtccagag tgccacgttt caggtggtcc agcagacggc ccggagtacc
    20161 gacaacgatc tgcggcccct gacgcagggc gcgtaattgc acgtcataac gctggccgcc
    20221 gtacagagca accacattta cgccgcgcat gtgtttagag aaatccgtca ttgcttcagc
    20281 aacctgtacc gccagttcgc gggtcggtgc cagcaccaga atctgtggtg ctttcagctc
    20341 aggatcaaga ttctgcagca gaggtaaaga gaatgctgca gtttttccgc tccccgtctg
    20401 ggccataccc agaacgtcgc ggccattcag cagatgtgga atacactctg cctgaattgg
    20461 agatggtttt tcgtaaccca gatcgttaag ggcttcaagg ataggagcct tcaggcccag
    20521 atctgcaaaa gtggtttcga attcagccat gtagtacgtg tgcctcaaaa ttaatggcgg
    20581 ccagtctaca taactcatca tgaaattgat cagcaatttt cattgaaaag tgtgaaccgg
    20641 ctcaaagtag gtgtattaac gaacaacaac gccctcaccc gttaaggtga tggcaatcaa
    20701 aaaagattac gggctgatgt gtacgtcagc tattgctggt ccgattctgc caggtcatct
    20761 tggtcctggc ccaggagcga taattccaac aatgcgtatc ggtgctcaac aaagttatga
    20821 acgttgttgg caaccgccag tttgaacagt gccgtggcgc tgtccaaatc ccccagactt
    20881 aggtagtact tacctaaata gaagttggtt tcactgagat gctcagcgag cgaggtgtta
    20941 tccgttgcgt ccgccttgag cctttccatt aacgtttgtt cgctaatgtt gcccaggtag
    21001 aactcgacaa tgttccatcc ccactgttcc ttatccgatt tttcgaagtg ctgtttcaac
    21061 acttctttag cctgcttctc atcgagcttc tgctcggcga gataaagcca cagactacgg
    21121 aaaggatcat tgggatcgtc ttgataaaac gccagcagat catcttgcgc taacttgtca
    21181 cgaccgccgt aatataatgc gatcccgcga ttcaagtgcg cgtagttgta agttggatca
    21241 agctcaagta cagaatcaaa cgcttcatag gcagcatcaa aattgcctgc ctgcgttaaa
    21301 tatatgccta agtaattgaa tacttcaggc atatccggtc ggattgccag cgcttgcgaa
    21361 aaatcgttac gcgctaatgc cctcagaccg agactatcat acaacactcc gcgctcatat
    21421 aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg caaggatttg ttccatacgt
    21481 gccagaatca cttcctgctg taaagtcggt tgcaatggta ccgcgaggac ttcactttta
    21541 cgccaggaag tattactgca tcctgcaagc gtaagtgctg tcgcaacgaa acaccagcgc
    21601 aaaaaaggct tcatttccca ctcccgaaga ccacggttga atgaacgtcc tgttcccggt
    21661 tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg cagcggaggg caaatggcaa
    21721 ccttactcgc cctgttcagc agccggagct tccggtgctg cagcaggttg agactgctca
    21781 gtcgcttctt taatgctcag acggatacgg ccctggcgat caacttccag aactttcacc
    21841 ggtacttcct gacccatctg caggtaatcg gtcactttct caacgcgttt gtcagcgatt
    21901 tgagagatgt gtaccagacc ttctttaccg ccgccgatgg caacaaatgc gccaaagtca
    21961 acgatacggg tcactttacc agtgtagacg cggcccactt cgatttctgc agtgatctct
    22021 tcgatacgac gaatagcgtg tttcgctttc tcgccgtcgg tcgctgcgat cttcacagta
    22081 ccgtcatctt cgatttcgat ggtggtgcca gtttcttcgg tcagggcacg gataacagaa
    22141 ccgcctttac cgataacgtc tttgatcttg tccgggttga tcttgatggt atggatacgc
    22201 ggtgcgaact cagagatatc gccacgcggc gcgttgatcg cctgttccat tacgcccagg
    22261 atatgcagac gcgcaccttt agcctggttc agcgcaacct gcatgatctc tttggtgata
    22321 ccttcaattt tgatatccat ctgcaatgca gagataccgt cgcgggaacc cgcaactttg
    22381 aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt cagacagtac aacgtagttg
    22441 tcgccttctt tcaccagacc cattgcgata cccgcaacgg cagctttgat cggcacacct
    22501 gcgtccatca gcgccagaga cgcgccgcac acggaagcca tagaagagga accgttggat
    22561 tcagtgattt cagacacaac acgtacggtg tacgggaatt tgtccatatc cggcatgact
    22621 gccagcacgc cgcgcttcgc cagacgaccg tgaccaattt cacgacgctt cggagaaccg
    22681 accatgccgg tttcgcctac ggagtacgga gggaagttgt agtggaacag gaaggtgtcg
    22741 gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac gagcagtacc cagcgttgcg
    22801 gtaaccagcg cctgcgtttc accacgggtg aacagcgcag aaccgtgagt acgcggcagc
    22861 acgccagtac gcacatccag accacgaatc atatcttttt cacgaccgtc gatacgcggt
    22921 tcgcctgcca gtacgcggct acgaacaacg tttttctcga tagcgtgcag aatttcaccc
    22981 agttcgtttt cgtccagggt ttcgtcttca gcaagcagcg tcgcgatggt ttcagatttg
    23041 atgacatcaa cctgcgcata acgctcttgt ttgtcggtga tgcggtaagc atcactcaga
    23101 cgagcttcag ccagtgcagc aacgcgcgcg ttcagcgctt cgtttaccgg ctccggctgc
    23161 cagtcccaac gtggtttacc ggcttctttc accagttcat tgatgttctg aataacaacc
    23221 tgctgttgtt catgaccgaa cactactgcg cccagcatct ggtcttcgct cagcaattca
    23281 gcttcagatt caaccatcag tacagcggct tcagtaccag caacaaccag atccagtttg
    23341 ctctctttca gctcgtcctg agtcgggttc agtacgtact ggtcattgat gtaacctacg
    23401 cgggcagcac caatcggacc attgaacgga ataccagaca gagacagtgc tgcggaagca
    23461 ccaatcatcg cgacgatatc cggattaact tgcgggttaa cagaaaccac ggtggcgata
    23521 acctgaactt cgttgacgaa gccttccggg aacagcgggc gaatcgggcg gtcaatcaga
    23581 cgcgcgatca gggtttcgcc ttcgcttggg cggccttcac gacggaagaa gctacccggg
    23641 atacgaccag cagcgtaagt acgctcctga tagttaacgg tcagtgggaa gaagtcctga
    23701 cctggtttgg cttttttctg gccaacaacg gtaacgaata ccgcggtgtc atccatgcta
    23761 accataacag cggcagtagc ctgacgagcc atcatgccgg tttccagagt cacggtgtgt
    23821 tggccgtact ggaatttacg aacgatcgga ttaagcaatg taatatcctt tctctttctt
    23881 agacagtacc ttacggcact ggtgttaata cccgatcttc tgcgcatcct cgcgactaat
    23941 gacaacccta acccagctct atgtgggtaa agcctctcat tagccgcgcg aacctctgca
    24001 acggaagatc attcatagca acaatacatt agtttccagt gaattgctgc cgtcagcttg
    24061 aaaaaagggg ccactcaggc ccccttttct gaaactcgca agaattagcg acgcagaccc
    24121 aggcgctcga tgagctgggt gtaacgtgct acgtctttac gtttcaggta gtcgagcagt
    24181 ttacgacgct gagaaaccat gcgcagcaga ccacgacggc tgtggtgatc ttttttgtgc
    24241 tctgcaaagt ggccctgcag gtggttgatc tgtgcagtca gcagtgctac ctgaacttcg
    24301 gtagaaccgg tgtcgtttgc gtcacgacca aactcagaaa cgattttagc tgttgcttca
    24361 gtacttagag acattttaaa actccaaagt atatagaatg aaaggacgcc gatctctaat
    24421 tcagcgatcc cagtgtacgt tacgcaaagt gttaaacaat ttacgcgacg ttaagcggca
    24481 gtattctact cgtagcgacc tgttatcgca agacggttaa cattacgccg ggtattcaac
    24541 caccaggcga cgaggcgcaa cgcggccttc atcgtcaatt tcgcccatac cgataaattt
    24601 gccgttctca ccttctgtaa cgcgaaccag tccttccagc ggcgcaccgg atgtacgaac
    24661 tgggttacca tttttgaagt aaacagaaga cgttaacgga agattcacca ccgggtagtc
    24721 cgaagctgga ctgtccattg gcatcagtaa tggatcaagt aactccgcag ccggaatatc
    24781 ctgctgttcg gcttgttcga ccagttcacg cagatgttcc agggtcacca tccgttcaac
    24841 cggatattta cttaccgcca gacgacgcag gtaaataaca tgcgcgccac aaccgagttt
    24901 ttcacccagg tcatcaatga tggtgcggat ataagtgcct tttgagcagt gaatttccag
    24961 ctccagctca ttgccttcgt ggcgaataaa cagcaattca taaacggtaa tcgggcgcgc
    25021 ttcacgcggt acttcaatgc cctgacgcgc atattcgtac agttttttac cctgatattt
    25081 cagtgctgaa tacatcgaag ggatctgttc gatatcgcca cggaaagtat ccagcgccgc
    25141 cgccagttgc tctgcgctaa aggttaccgg acgttcttca acgatttgtc cgtcggcatc
    25201 agaagtatcg gtacgctgtc caagacgcgc aatgacccga tagcgtttgt cggagtccag
    25261 cagatactgg gaaaacttcg tcgcttcccc gaggcaaatt ggcaacatgc cagttgccag
    25321 cgggtccagc gcaccggtat gcccggcacg gttggcgtta taaatccgtt tcactttttg
    25381 cagcgcatca ttgctggaca taccctgcgg tttatccagc aacaaaacgc cgttaatgtc
    25441 gcgaccgcga cgacgaggac gactcattag tcctccttgc tgtcgtccgg gttaacacga
    25501 cgttcttcgt catgtttgac cacgctggtc accaggtttg acatgcgcat cccttcaacc
    25561 agagagttgt cgtagaagaa ggtcagttcc ggcacgatac gcaggcgcat cgctttcccc
    25621 agcaggctgc ggatgaaacc agaagcttct tgcaacgctt tgatgcccgc tttaaccgcg
    25681 tcttcatctt tgtcgttgag gaacgtcaca tatactttgg catacgccag gtcgcgagac
    25741 atttcgacac cggaaacggt ggtcatcatg cccaggcgag gatctttaat ttcacgctgc
    25801 aggatgagag cgatctcttt ttgcatttcc tgcgctacgc gctgcgggcg accaaattct
    25861 ttcgccataa taaattctcc tgacaaaaaa ggggctgtta gccccttttt aaaattaatt
    25921 tcaggtggaa gggctgttca cgttggcctg ataagacgcg ccagcgtcgc atcaggcagt
    25981 ccatgcagga tgcggcgtaa acgccttatc ccgcatggaa ccctaaaaac cttaagcgat
    26041 ggtacgttgg atctcgatga tttcgaatac ttcgatcaca tcgccagtgc ggacgtcgtt
    26101 gtagttctta acgccgatac cacattccat accgttacgg acttcgttaa cgtcatcttt
    26161 gaagcggcgc agggactcca gctcgccttc gtagataacc acgttgtcac gcagaacgcg
    26221 gatcgggttg tgacgtttaa ccacaccttc ggtaaccata cagcctgcga tggcaccaaa
    26281 tttcggtgat ttgaacacgt cacgaacttc cgccagaccg ataatctgct gtttcagttc
    26341 cggagacagc ataccgctca tcgccgcttt cacttcgtca atcaggttat agatgacgga
    26401 gtagtaacgc agatccaggc tttccgcttc aatcacttta cgtgcagagg catcagcacg
    26461 tacgttgaag ccaaccagga tggcgttgga cgccgcagcc agggtggcgt cggtttcggt
    26521 gataccccct acgccagaac cgatgatctt cactttaact tcgtcagtag acagtttcag
    26581 caaggagtcg gagatcgctt ccacagaacc ctgtacgtct gccttcagga cgatattcac
    26641 ttcgtgaact tcgccttcgg tcatgttggc aaacatgttc tcgagtttag atttttgctg
    26701 acgcgccagt ttaacttcgc ggaatttacc ctgacgatag agtgcaactt cacgcgcttt
    26761 cttctcgtca cgtacaacgg taacttcatc acccgcagcc ggaacgccgg acaggccgag
    26821 gatttccacc ggaatggacg gacccgcttc cagcacttcc tgacccagtt cgttacgcat
    26881 cgcacgaaca cgaccgtatt cgaagccaca cagaacgata tcgcccttgt gcagagtacc
    26941 ttcacgtacc agaacggtag caaccggacc acgaccttta tcgaggaagg attcgataac
    27001 cgcaccgctc gccatacctt tacgtaccgc ttttagctcc agaacttccg cctgcagcag
    27061 gatagcgtcc agcagttcat cgataccggt acccgccttc gcagatacgt gtacgaactg
    27121 gctttcaccg ccccactctt ccggcaggat gccatactgg gagagttcgt tcttaacacg
    27181 atccggatcg gcttctggtt tatcgatctt gttcactgca accaccaccg gtacctgcgc
    27241 cgctttcgcg tgctggattg cttcgatggt ctgcggcatc acgccgtcgt cggcagcaac
    27301 aaccaggact acgatgtccg ttgcctgcgc accacgtgca cgcattgaag taaacgcagc
    27361 gtgccccggg gtgtccagga aggtgatcat gccgttttca gtttcaacgt ggtatgcacc
    27421 aatgtgctgg gtaatgccgc ccgcttcgcc agaggccact ttcgttgaac gaatgtagtc
    27481 cagcagagag gttttaccgt ggtcaacgtg acccatgatg gtcacaaccg gcgcgcgcgg
    27541 ttcagccgca gcccccgtgt cacggtcgct cattaccgcc tcttccagct cgttttcacg
    27601 acgcaggata actttgtggc ccatctcttc agcaaccagc tgtgcggttt cctgatcgat
    27661 aacctggttg atggttgcca ttgcgcccag tttcatcatc gctttgatga cctgagagcc
    27721 tttaaccgcc atcttgttcg ccagttcgcc aacggtgatg gtttcgccga tcacaacgtc
    27781 acggttaacg gcctgagctg gcttctggaa gccttgctgc agggaagaac ctttacgttt
    27841 tccgccttta ccgccacgta ctgctgcgcg tgcttcttca cgatcagctt ttgattcagc
    27901 gtgtttgttg cctttcttcg gacgcgctgc tttcgcgtta cgaccacggc cacggccgcc
    27961 ttcgacttca cgatcgcttt cgtcttctgc ctggcgagca tgttgagaag ttgtgacatg
    28021 ataatcgctg gaatcttcag tcggttccgc gttatcagtc catttgtttt cttccgccat
    28081 acgacgtgct tcttcagcaa cgcgacgtgc ttcttcttcg agtttacgac gcgcttcttc
    28141 ttcagcttta cgcttgagct ctgcagcttc ctgctcacgg cgggcttttt cagcctgggc
    28201 gtttttagtc atatcgtctt gttgattgct cactttgtct ttttccgcag cttcacgttt
    28261 cgcttgttca gcagcttcac gcttagcttg ttctgcggcc tcacgttcag ctttttgttg
    28321 cgcctcgcgt ttagccgatt cttctgcctc acgacgggct tgctcttccg cttcacgctg
    28381 cgcttgctct tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg
    28441 tttcttgcgg acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag
    28501 ggtgctgcgt gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag
    28561 gtggtcaatc aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat
    28621 acctgcatca gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag
    28681 cgttttaatc gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc
    28741 accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa
    28801 cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac
    28861 gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc
    28921 gtcagccggt ttgttatcac cgaggctttc ttccagggcc tgtgcaatgg tggccagtgc
    28981 atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa
    29041 cagctctttc atcggaacat aggccagttc ttccagcgtc gagaaacctt cttctaccag
    29101 aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg
    29161 cgcttccgcc tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc
    29221 gctcagctgc gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt
    29281 accggcttca acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac
    29341 gtctgccggt gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat
    29401 atcgatacgc tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc
    29461 tacacaggca cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg
    29521 agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttctggcac
    29581 ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc
    29641 gccacgcgct tccgggcgaa cggaatagag cacgccacga acgcggtcgc cagggcggaa
    29701 gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcattgt tgccgagatc
    29761 caaagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg
    29821 ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac
    29881 ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct gatcttcaac
    29941 gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt
    30001 ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct
    30061 tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct ttgttgctgt
    30121 cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga
    30181 tacggcttca actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga
    30241 ctattaaaag tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc
    30301 tttaccttcg acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca
    30361 tttacgacgg ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg
    30421 ggcgtagtgt tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt
    30481 ataagcaacg gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc
    30541 agcacaatca tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt
    30601 gcgaccgcga ataaattcga tgccaaccag ttcaaaaccc agggcctcaa ctggcgcagt
    30661 aatcatctct gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa
    30721 gggcttaaag cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt
    30781 tatataactg aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt
    30841 caaatcccac tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa
    30901 agtggttgcg ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac
    30961 caggctgctc caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaagatg
    31021 caaatactgc tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg
    31081 atccatgcca cattttgtca acgtttattg ctaatcacgt gaatgaatat ccaattcact
    31141 ttcatttgtt gaatactttt gccttctcct gctctccctt aagcgcatta ttttacaaaa
    31201 aacacactaa actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt
    31261 gcataaaaat tcagtgagag cggaaatcca ggctcatcat cagttaatta agcagggtgt
    31321 tattttatga cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt
    31381 tctggcggtc tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct
    31441 tatgcatata ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt
    31501 cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg
    31561 gccgaaggta ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc
    31621 tatttcaaca cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg
    31681 aaagaagatg gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa
    31741 cgtttctatc gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt
    31801 gatactgact ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc
    31861 tgcggtttcg actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt
    31921 ggtgcaacgc atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac
    31981 ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca
    32041 gtacgctttg agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta
    32101 gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag
    32161 attgaaaacc gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca
    32221 ctgctgcaca ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag
    32281 cagtatcacg cgcatggtcg tcagttgggc cgtctgctgt atcaggggcg ttggtttgac
    32341 tcccaggcgc tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt
    32401 gaagttaccc tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag
    32461 aacctgacct acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca
    32521 gatgatcgta ttggtcaatt gaccatgcgc aacctggata tcaccgatac ccgcgagaaa
    32581 cttttcggtt atgccaaaac tggcctgctc tcctcctctg ccacttcagg cgtgccgcag
    32641 gtggagaatc tggaaaataa aggccagtaa ttcgacatca accctgccct tcaggggtgg
    32701 ggttaccttg cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat
    32761 ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tccagcgttt
    32821 gcggctgttc cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtacgcag
    32881 tggaaaagat atcgttttct gttgtgcgat ccacgccatc cccaagaacg gggctatacc
    32941 agataaacat cgggacatga tatgcctgct ggctggcctc cctgcctcca tgaaaataga
    33001 cgttcttctt cgtagggtcg cgttccaggc cgtggtcggc aaaatacatg accgaggcgc
    33061 ggcgatcttt tagtaattca aaaacctgac ctagcaaact gtctgtgtaa tgaatggagt
    33121 tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg
    33181 cgctacaagc cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg
    33241 tcttttgctg taacgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat
    33301 agactgtttc catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag
    33361 cggattgcga gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt
    33421 tgtcggggta attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa
    33481 ccgacagcgc agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc
    33541 tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga
    33601 cacgtacaga ctcccccaca atcaacacgt aggtatcaat acctgtatcc ctgactgata
    33661 attgaaaata cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta
    33721 aagcaaaata gttgagatta aaaaacggcg tataggtagc aaatcgcgac gctagtatat
    33781 atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata
    33841 aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa
    33901 catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat
    33961 aagggatata catccccagc attttgacaa cttcatccgg atcactctgc agcacactaa
    34021 tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca
    34081 tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg
    34141 atatacgttt aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg
    34201 ctccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc
    34261 aatgagattt aaaacttcta gcaaatttgt tgaatactgt catccagaaa tccaagttca
    34321 caaccaatat aaacaattat caacaatgtt caattttata tcagacatgt ttttcaactc
    34381 tgatccatat cagaccgaaa cactgttagt tttttctttt taaatgatgc ggccattgtt
    34441 ctcagtaatt aaataacatt atcaatgcgt ctctaaacat tacgcagaac gtagccatca
    34501 gtagttatac tgacaggagc aggatggttt atcgagaagt aaacatatcc gtattcgtac
    34561 cggaactgga gttgacacaa taaagtgcca attatgtcag cagtagggaa aagtttataa
    34621 ctaaggcgta ccggcaccat cgtttcaaag taccagctac gagtaaagca actggacgag
    34681 atacagatac ctgacaacca ttcctcagac caggaccaaa acgaaaaaag acgcttttca
    34741 gcgtctcttt tctggaattt ggtaccgagg acgggacttg aacccgtaag ccctattggg
    34801 cactaccacc tcaaggtagc gtgtctacca attccaccac ctcggcacgg atactacttt
    34861 ttagttcggg atatcgctgg tcggcttagc cggagcagct ggctgagttt gttcggtttt
    34921 cgccggcgca ctcaggtttt cccattcgct acctttattg gttttattgc tattgatgtt
    34981 acccagcacc agactgatga tgaagaataa cgttgccagc agcgccgtca tgcgggtcat
    35041 gaagttacca gaaccacttg aaccaaacag cgtagcggaa gcgcctgctc cgaaggaggc
    35101 tcccatatca gcgcctttac cttgctgcag catgatcaga ccaacaaggc caattgccac
    35161 aataaggaaa actactaaaa gagcttcgta cataatcaac ctgttccttg cggagttgcc
    35221 gcgtaccaat gcttcaacca ataaagcggg agtttttaat ctttcccact gaagcgggtg
    35281 tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt ttatcgcatt gtatcaactg
    35341 cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa aggcgacaat cgccgccttt
    35401 ttagccagtt atctaacgct ttaaacggct tttactgcat cggcgatgcg gtgtgcaaat
    35461 tcagtcacct gcgcttcgtc ttcgccttcc accatcacgc gaattaacgg ttcggtgccg
    35521 gatttacgca gcaacacgcg tccacggttg cccagcgcag cttcaacctc tgcggtcacg
    35581 gctttaactg actcatgctc aagtggatcg ccgctacctg cggtgtaacg tacgttaacc
    35641 agaatctgcg ggaacatttt catgccgctg caaaggtcgt gcaggctcat atggttacgt
    35701 gccatcgccg ccagcacctg caagccagca acgatgccgt caccggtagt agttttatcc
    35761 agtaggatca catgaccgga attctctgca ccgatacgcc agcctttctc ctgcattttt
    35821 tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg gaattcctaa ctgtttcagc
    35881 gccagttcaa gccccatgtt gctcatcaat gtacccacag cgccaccacg cagctggccc
    35941 tgacgaagac cttcacgcgc gatgatatac atgatctgat cgccatcgac tttattgcct
    36001 tcatggtcga ccataatcac gcgatcgcca tcgccgtcga aggcaatacc gagatccgct
    36061 ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg tagcccccac ttcggcattg
    36121 atgtttacac cgtttggctc acaaccgata gcgataacgt tcgcccccag ttcgcgcagc
    36181 acgttcggcg cgatgtgata agtcgcaccg tttgcacaat ccaccacaat cttcagttca
    36241 ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact cgatatagcg acccgcggca
    36301 tcaacgatac ggctggcttt acccagttct gccgaatcaa cgcagctgat ctccttttcc
    36361 atttccgctt cgatggcctc ttctaccgca tccggcagtt tggtgccgtc gatagagaag
    36421 aatttaatcc cattatcgta gaacgggtta tgcgatgcag atatcacaat tccggcctct
    36481 gcgcggaagg tacgcgtcag ataagccacg gccggtgttg gcatcgggcc agtgaagagt
    36541 gcggaaaggc ccgctgccgc cagacccgct tccagtgctg actccagcat atagccagaa
    36601 atacgcgtgt ctttaccaat aataatctta cgggagccgt ggcgcgccag cactttaccc
    36661 gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg gcgcatcccc tacacgacca
    36721 cgaatcccat cggtaccgaa atatttacga ttactcatag cgtttgtttt cctttgcaga
    36781 cagagtggct tccaccaccc gcatcgcttc tacggtttct ttgacgtcat gaacacgaat
    36841 gatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc agactaccgc tcaggcgctc
    36901 ggacggcacc acgttcagca gctgcccaat catcgatttt cgtgacatac ccaccaacag
    36961 cggcaggttg aaatggtgaa attcagccag gcgcgccagt aatgaatagt tatgggagag
    37021 atttttaccg aaaccgaatc cggggtcgag caacaatttc tcttttgcga tacccgcctg
    37081 ctcgcaacgt gctatttgct caataaagta gcgattcact tctgcaaaga catcgtcata
    37141 cttcggagct tcctgcatgg tttttggatt tccctgcata tgcatcagac aaaccggtaa
    37201 accggtttct gcagccgcct ccagagcgcc aggttcggaa agggagcgga tatcattaat
    37261 aatgtgagcg ccaactttcg ctgactcacg gatgacttct ggtttggatg tatcgaccga
    37321 gatccagact tcgaagcgtt gagcaattgc ctcaaccaca ggaataacac gttgcaactc
    37381 ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac tcgccaccaa cgtcaatgat
    37441 cgtcgcgcca gcattgatca tcagattcgc atgtttcacc gcatctatca gcgagttatg
    37501 cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg atccccatta cgtgaggatg
    37561 gctaaggtcc agtgaagtac cctgggcaaa gagtttcatg atgttatccc tggtatgaat
    37621 tgataagaaa aaaccccgga gcacgccccg gggttttcgg tacaaataca gtcatctgat
    37681 gcgggaactt acttgtcgcc taactgctct gacatggtgt tacccgggtt cggcgtacgc
    37741 ggttcatcaa ccggacgagg agcctttgga ctaccattgt cgccagaatt gttagaagcg
    37801 cctggttctt cccagcccgc tggcggacgt acatcgcgac gtgccatcag gtcatcaatc
    37861 tgcggtgcgt cgatagtctc atatttcatg agagcatctt tcatcgcatg cagaatatcc
    37921 atattgtcgg tcagaagctg acgcgcacga ttatagttac gctcaatcag tgctttcact
    37981 tcctggtcga tgatacgtgc agtttcatcg gacatatgtt tcgctttcgc tacgctacgg
    38041 ccgaggaata cttcaccttc ttcttccgcg tacagcagtg gacccaattt ctcagagaag
    38101 ccccactggg tcaccatgtt acgtgccaga ttggtcgcaa ctttaatatc gttggacgca
    38161 ccggtagaaa catgttccgg cccatagatg atctcttctg ccagacgacc accgtacagc
    38221 gtagaaatct ggctttccag tttctgacgg ctggcgctga ttgcgtcgcc ctcaggcaag
    38281 aagaaagtca cacccagcgc acgaccgcgt gggataatcg tcactttgtg caccggatcg
    38341 tgttccggca ccaggcgacc gataatcgca tgacccgctt cgtggtaagc cgttgattct
    38401 ttctgcgctt ccgtcatcac catggagcga cgttccgcac ccatcatgat tttgtctttc
    38461 gctttctcga actcaaccat cgacacaacg cgtttgttgc cacgagcagc gaacagtgcc
    38521 gcttcgttca ccaggttcgc caggtcagca ccggagaaac caggagtacc acgggcaatg
    38581 attgccgcgt cgatatcggg ggccaatggt acgcgacgca tgtgaacttt caggatctgc
    38641 tcacgaccgc gaacatctgg caagccgacc acaacctgac ggtcgaaacg gccaggacgc
    38701 agcagggccg ggtcgagaac gtccggacgg ttagtcgcgg cgataacgat gataccttcg
    38761 ttaccttcga agccatccat ctcaaccagc atctggttca gagtctgttc acgttcatcg
    38821 tgaccaccgc ccagaccagc gccacgctgg cggcctacgg cgtcgatttc atcgataaag
    38881 atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt cacgaacacg ggatgcaccc
    38941 acaccgacga acatttctac gaagtcagaa ccggagatag taaagaacgg aactttcgct
    39001 tcgcctgcaa tcgctttcgc cagcaacgtt ttaccggtac ccggaggacc gaccatcagg
    39061 acgcctttcg ggatcttacc gccgagtttc tggaagcggc tcggctcgcg cagatactct
    39121 accagttcag caacttcttc ttttgcttcg tcgcagcccg caacgtcagc aaaggtcgtt
    39181 ttgatctgat cttccgtcag catgcgcgct ttgctcttac caaacgacat ggcacctttg
    39241 ccaccgccgc cctgcatttg acgcatgaag aagatccaga caccaatcag caacagcatc
    39301 gggaaccagg agatgaagat agaagccagc aggcttggtt cttcaggcgg ttcaccgaca
    39361 accttgacgt tcttggtcaa caggttatcc agtaatttcg gatcctgaac cggaatgtaa
    39421 gtggtataac ggttactatc tttcttggta acgttgattt cacgtccgtt gatacgcgct
    39481 tcacgaacct ggtcgttatt gacctcttgt aggaaggtag agtaatccac cttacggcca
    39541 ttagactcgc tgggcccaaa gctctggaat actgacatca gcacaacggc aatgaccagc
    39601 cagagtatta ggtttttcgc catgtcactc aagggattaa cctcttatta caactgtgtt
    39661 aaaaacagcg tcaggatact ctatatccag catctttcaa actttcgtct gaaatctccc
    39721 ggttagggtt tacgcccggt cgctacaata tacacttccc gcgaacgtgc acgagaagag
    39781 tccggcttac gaactttgac cttcgtaaac agggagcgaa tttcccttag atactcatcg
    39841 aaaccttcgc cctggaacac cttcactaca aaactgccac ctggcgctaa tacatcacga
    39901 cacatttcta acgccagttc caccagatac atggcacggg ggatatccac cgccggtgtt
    39961 ccgctcatgt ttggtgccat atcggacatg acaacctgga ctttgctgtc gccaacgcgc
    40021 tccagcagtg ctttcatcac cagttcatca cgaaaatcgc cctgaagaaa gtccacacca
    40081 acgataggat ccataggtaa aagatcgcaa gcgatgatgc ggcctttgcc gccaatttgg
    40141 gtgaccacat attgtgacca accacccgga gcagcaccaa ggtcgacaac cgtcattccc
    40201 ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt taaaccaggc acgggaacgt
    40261 aacccctttt tctgtgcctg ttgaacatat ttatcgctaa agtgttcctg aagccagcgg
    40321 ctggagctgg cagaacgctt cttacctgtc atttaacttt cccatgggga taactcatcg
    40381 taaccaattg cgtaaatttt tacgcgccta tttggtgata tatgggagat ggcggtagaa
    40441 tgacccgttt tcaatcccaa cgtaagcaaa aaatacgatg aatctgagta ctaaacaaaa
    40501 acagcacctg aaaggtctgg cacatccgct caagccagtt gttctgcttg gcagtaatgg
    40561 tttgaccgaa ggggtgctgg ccgagattga acaagcgtta gagcaccatg aactcatcaa
    40621 ggtgaaaatc gccaccgaag atcgcgaaac taaaaccttg atcgtggaag ctatcgtgcg
    40681 cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg ctggtgcttt atcgcccaac
    40741 taaagaacgt aaaatctcgc tgccacgcta agattatcct aaagttacac acatttgctg
    40801 tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg ccacgctctg ttcgttgata
    40861 aaaggccgca tagcggcctt tttcctttct ttacaataca tcaacatctt gagtattggg
    40921 taattcttac aggtattcca ccttaattac ttcaaattct acttcgccgc ccggcgtttt
    40981 gatgaccaca acatcatctt cttctttgcc gatcaggcca cgagcaatag gcgagtttac
    41041 agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca acgatgcgat aagtctgttc
    41101 ttcgtcagaa tccagattca gcaccgttac ggtagcacca aaaataacgc gcccattgtt
    41161 gggcattttg gtgacatcaa tcacctgcgc gttcgacagc ttggcttcga tgtctttaat
    41221 acggccttcg cagaaaccct gctgttcacg agctgcgtgg tattcggcgt tttctttcag
    41281 gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt tcaggacggc gcacagattt
    41341 cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt aaggtcatcg gaatagcttg
    41401 catttgttat acctcttgaa tattcctgat ggggcaagtc ttcacccacc ccggctgttc
    41461 ggcctgcccg gcataactcc tcaccgggac cagaagcaaa aaaataccga cccgggtaca
    41521 agtcccaggt cagctacaat tcacattttg atagtcattt taccctgaag ttcccgaagg
    41581 gtcatcgttt actttatagg gcgttgcgcc gtagtatgac ggctcgattc caggttgtta
    41641 gcgcgagatt atgcgatttt ccagatttat catcggattg accagctgta tagcgttcag
    41701 tgttcaggcc gcgaatgttg atgagtacgt tactcaactc cccgctggtg ccaaccttgc
    41761 cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt gattaccaca gtcagcagat
    41821 ggcgctgccc gccagtacgc agaaagtgat tactgcgctg gcggcgttga ttcaactcgg
    41881 ccccgatttt cgtttcacca cgacgcttga aaccaaaggc aatgtggaaa acggcgtact
    41941 taagggtgac ttagtggcgc gatttggtgc cgatccgacg ttaaaacgtc aggatattcg
    42001 caatatggtc gcgactttga aaaaatctgg cgtcaaccaa atcgatggca atgtgttgat
    42061 agatacctcc attttcgcca gccacgataa agcccccggc tggccatgga atgacatgac
    42121 acaatgcttt agcgctccgc ctgccgctgc catagttgac cgcaactgtt tctccgtctc
    42181 gctctacagt gccccaaaac ctggtgatat ggcttttata cgcgtggcat cttattaccc
    42241 cgttacgatg ttcagccagg tacgcaccct cccccgaggt tctgccgaag cgcaatactg
    42301 cgaactggat gtcgtaccag gcgacctgaa ccgctttacg ctgacgggat gcctgccaca
    42361 acgttctgag ccgctcccgt tggcttttgc cgtgcaggat ggagccagct atgccggggc
    42421 aattctgaaa gatgagttaa aacaggcagg tatcacctgg agcggaacac tgctgcgcca
    42481 gactcaggtt aacgaacctg gaacggtagt tgccagtaaa cagtcggccc cgttgcacga
    42541 tctgcttaag attatgctga aaaagtcgga caacatgatc gccgatacgg ttttccgcat
    42601 gataggccat gcgcgcttca atgtgcctgg aacatggcgg gccgggtcgg acgccgtgcg
    42661 tcagatcctg cgccagcaag ccggtgtcga tattggaaac accattattg ccgatggttc
    42721 agggctttcg cggcataacc tgattgcccc cgccaccatg atgcaggtgc tgcaatacat
    42781 tgcccaacac gacaatgaac ttaactttat ctccatgctg ccgcttgcgg gctatgacgg
    42841 ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg gatggaaaag tctcagcgaa
    42901 aaccggttcg ttgcaggggg tatataacct ggcgggattc attaccacag cgagcgggca
    42961 acgaatggcg tttgtacaat atctttctgg ctatgcagta gaaccggcgg atcagcgtaa
    43021 tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat aaagatattt atcagaacaa
    43081 ttagtcaaaa agaaaccccg gcacatggcc ggggttttgc tgattaacgc ttgtaaatga
    43141 actcaacgcc ttcttcgtcg tcttcgtccc agtcatcatc ccagtcctca tcatcctctt
    43201 cagcaatctc ttcaagctgc tggcgatgat aatcatccca catgaattcg actttctctg
    43261 gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat gataaaggtc atcacatccc
    43321 agcagagatc tttcacgccc agtccactcg ccgcagagat cagataatat ttatcttccc
    43381 agcccagcgc ttcagcaatc gctttcgctt tctcttcagc ttctgcctta tccagcagat
    43441 cgatcttatt gaacactaac caacgtggtt tcgccgccag atcctggctg tatttttcca
    43501 gctcgctgat aataatacgc gcgttttcaa ccggatcggt gccgtcaatc ggatcgatat
    43561 cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt caggaagcga atgcccagac
    43621 ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc ggcaacaacg aagctctttt
    43681 cgttgtccat tcgtaccaca cccagacttg gcaccagagt ggtaaacgga taatccgcca
    43741 ctttcggttt agccgccgat accgcacgaa taaaggtcga tttacccgcg tttggcatcc
    43801 ccaacatacc gacgtcagcc agcagcatca gctccagcag cagctcacgc ttatcgcccg
    43861 gcgtaccgtt agttttctgc cgtggtgtac ggttaactga cgatttgaaa cgggtattgc
    43921 ccagaccgtg ccagccgccc ttagcaacca gcagacgctg accgtgtttg gtcatatcgc
    43981 ccatggtttc accagtaccc tggtcgatta cacgcgtacc taccggcact ttaattgtga
    44041 cgtctttacc gcgcttaccg gtacagtcgc ggcttgcgcc attctgaccg cgctctgcac
    44101 ggaaagattt ttcaaaacga taatcgataa gcgtgttcag gttctcgtcg gcttccatcc
    44161 atacgtcacc accatcaccg ccgtcgccgc catccgggcc gcctttcgga atatactttt
    44221 cgcggcggaa gctcacgcaa ccattaccgc catcacctgc aacgaccaga atcgatgctt
    44281 catcaacaaa cttcatttta ttctccgtaa atcattcgcc tgcgcggggt tgcgatacca
    44341 ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc aatggcggaa tacatcgcgc
    44401 ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt taacatcggt ctggcgaaga
    44461 aatcgggtca ggccagtgat aaaagatctg aaaaaaacag cgtaaacagt ggggtgagcg
    44521 tgatgatcgc gctcacctgc gctgcctgcc agcgagccat cgcttccgcc agggcgccat
    44581 atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc gagctgccag tggctaagct
    44641 gcgctatcac tccaggcttt gccagaggga agagcgcaat tgtacataaa gtgtacagta
    44701 aaaacaggat ctgcggtgag gccagccgac gcaataaaac cttttgcgcc acgccataac
    44761 tcacccaaac cgtcgccgca ccgaccccaa agataactcc ccaggtgtaa tcggtgagct
    44821 ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac caggccgctc aggagcatca
    44881 atgcccctac aacctgagtg ctgcgcattt tctctttcag gataaatacg ctggcaacca
    44941 tcatgccaac tggcgagagt tgcccaatca cctgcgaagc ggtcggactc aggtattgca
    45001 aggatgagct gaacaggatg aagttcccaa acagcccggc ggtcgccact gccaacaaaa
    45061 tcaaccagcg tggcttacga aacacgcgta atggcggcaa cctcttcttc accgcaagaa
    45121 tggcaccaag gccaatactc gccatcaaga aacggtaaaa cacgattgtc ggaggttcca
    45181 tcacctccag cacctgcttc attgcgattg gcaacgcccc ccagcaaatt gctgtggtga
    45241 gcgccaaaag aatgccaatg cctgcctgct gcttcatacc gtattccctg caaagcgcat
    45301 ttgcggatta acgtcgcacc atgcgcattt ttaccggtta tcgaatgtaa aaagccccgc
    45361 aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa aacttattca gcttcgatgc
    45421 tgataaattt acggtttttc gggcctttaa cttcgaattt cactttaccg tctgctttag
    45481 caaacagagt gtggtcacga ccgcaaccta cgttagcgcc agcgtggaat ttggtaccac
    45541 gttgacgaac gatgatgcta cccgccagaa cggattcgcc accgaaacgc ttaacgccca
    45601 ggcgtttagc ttctgaatcg cgaccgttac gtgtggagcc gccagccttt ttatgtgcca
    45661 tttgaaatct ctcctcaggt cttaggcgct gatgccagta attttcacat cagtgaacca
    45721 ctgacgatgg ccctgctgct tacgatagtg tttacgacga cgaaacttaa cgattttaac
    45781 tttctcgcca cgaccgtgag caacaacttc agctttgatt acgccgccat caacgaaagg
    45841 aacgccgatt ttgacttctt caccgtttgc gatcatcagc acttcagcga actcaacagt
    45901 ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc tgaccttcgc ttactcggtg
    45961 ttgtttacca ccactttgga aaaccgcgta catataaaac tccgcttccg cgcacacctt
    46021 ttcaatgatt cagagtgcgc tataaatatt cacaataggg cgcgaatatt acgcaaaacg
    46081 cacgcctttg acaagtgcta cagtcaatac acgaagaaaa aaaacacaac ttgtacggta
    46141 acgtttatct gtgccatttt ttcagtacaa tcactctata ttcctaacca taaaccctaa
    46201 gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta
    46261 gaaaaaatca atgagttaac cgcgcaagat atggcgggtg ttaatgcggc aatccttgag
    46321 cagcttaatt ccgacgtcca gctgatcaat cagttaggct attacatcgt cagcggcggc
    46381 ggtaaacgta ttcgtccgat gattgctgta ctggctgcac gagctgttgg ctatgaggga
    46441 aatgcgcatg tcaccattgc tgccctgatc gagtttatcc acacggcgac tctgctgcac
    46501 gacgacgttg tggatgaatc agatatgcgc aggggtaaag ctaccgccaa cgctgcattt
    46561 ggcaatgccg ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt ccagatgatg
    46621 accagcctcg gttcgctcaa agtgctggaa gtcatgtcag aagccgtaaa cgtcatcgca
    46681 gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg acatcactga agaaaactac
    46741 atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg ctgccgcgca gtgttccggg
    46801 attctggctg gctgtacgcc ggaagaggag aaaggcctgc aggattatgg gcgctatctc
    46861 ggcactgctt tccagttgat cgacgattta ctcgattaca atgccgatgg cgaacagtta
    46921 ggtaaaaatg tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg
    46981 atgcatcatg gcacaccaga acaggcacag atgatccgta ccgccatcga acagggtaac
    47041 ggtcgccatc ttctggaacc ggttcaggaa gcaatgaacg cttgtggatc tcttgaatgg
    47101 acgcgtcagc gtgccgagga agaagcagac aaagccatcg cagcattaca ggtgctcccg
    47161 gacacccctt ggcgagaagc actcatcggc ctcgcgcaca tcgctgttca acgcgatcgt
    47221 taatcccctc ccttcatccc gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac
    47281 actaattcag cgctataaca cctgaaaaaa agggcgatga atctttatat attctgaata
    47341 ttcacactct ttacaggaac tttttagagc aataggccat caggagtata gtgatgcccg
    47401 acagaagaag tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta
    47461 atttcattga ctggcatccc gctgacatca ttgcgggttt gcgcaagaag ggaacgtcaa
    47521 tggcggcgga gtctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc
    47581 gcccatggcc gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta
    47641 tctggccatc acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga
    47701 tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct
    47761 gccagctctc agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca
    47821 gtttgtcttc aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg
    47881 tcccttccgc aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca
    47941 taacctgtgc gccagaaagt ttttcaacac cgtgacaaat aacggtattg ctttcgattt
    48001 cggcatgcgc gcccatacgg ctaagctccg gcacatgcat aaagcggttt tcaaagaccg
    48061 tttcggtgat gaatccggtc ccttctgcca ccaggttcaa cagcgtgaac tgggcctgca
    48121 tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc
    48181 catgcatatc caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcgtcacgca
    48241 gtttcgccag cacggcgtcg agagtatctg gctgcgcgtt acggcagata attttgccgc
    48301 gagaaatcgc cgccgccacc aggaaagtac cagtttcgat acgatccggt agcacgcgat
    48361 agacaccgcc gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc
    48421 taattttcgc acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg
    48481 ctgcgttttc aataatcgtg gtaccttccg ccagggttgc agcacacatg atggtcaccg
    48541 ttgcgccaac gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg
    48601 aagctttaac gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa
    48661 tgtgtagatc aaccggacgc gcaccgatcg tacaaccgcc aggcagtgaa acttgcccct
    48721 gaccaaagcg cgctaccagc ggccccagtg cccagataga agcacgcatg gttttaacca
    48781 gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac
    48841 gttctacttt cgcacccagc tggcttagca gcttcattga tgtatcgacg tctttcagtt
    48901 tcgggacgtt ctggatctct accggttctt ccgccagtag agcggcaaaa aggataggca
    48961 gagcagcatt tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa
    49021 cacgaaattt atccatttag tttgttctca gttaacaatt catatccgct accggcgaat
    49081 cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg
    49141 ctttgatcga cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga
    49201 ccgtctgctg ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg gcaataacct
    49261 gaaagtggct gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca
    49321 cgctctgaat ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa
    49381 catcttagag caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac
    49441 aaggctcgac ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa
    49501 aaatttaacg aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt
    49561 tgtcgtttac cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat
    49621 ggagcagcag tgccagtcca cccgtatcca cgcgggacac acggctaaga tcgatgcagg
    49681 taatcccctt caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca
    49741 gctctccgga taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta
    49801 ttttttctct tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc
    49861 gtcgatacct ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact
    49921 gacgccttca gcaatcatgt cgtaagcctg ccagttgccc gtctgggagt ttttacgcca
    49981 ctggaagtcc agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg
    50041 cacaatggtt ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata
    50101 catcgccagc gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc
    50161 ttcacgttga gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta
    50221 tttcacctgt acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt
    50281 ggcccgaatt tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc
    50341 catcagctta taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac
    50401 cagcaaagcg accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt
    50461 tgtacccaca ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact
    50521 attcttattg tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac
    50581 catcgcagac ttagtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc
    50641 cgggtcttca aaaccgacgt ttaatgccag atattgttcc cccagcaggc cggaagtacg
    50701 aatgctcagc gaactggtat ctggaatgtg gttataacgt tgttcaattt ccagcgttac
    50761 gcgcggcaga taggttttcg ggtccagcgt aatatccgcc acccgaccca caacaacgcc
    50821 accaatactg accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag
    50881 cgtgtaggtc ggttcagtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag
    50941 cgccgccagc agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg
    51001 catgaactca attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca
    51061 gagacgagtg gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg
    51121 cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa
    51181 tcagacagtt gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga
    51241 acccgctatc aatgcctttc caactgacgc cgaccagaga tccgccccag atccccacgg
    51301 cgacgaaaat aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa
    51361 taacccgacg cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc
    51421 gcatcaggcc gatttctgcg gttagcgccg aaccagcacg cccggcaaac aacaacgcgg
    51481 caaccaccgg ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt
    51541 ccgcactata agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca
    51601 cgccagaaac cacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca
    51661 gcagcggcgc atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc
    51721 cggcccgccc gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat
    51781 ttaacagcat gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga
    51841 tagcggaacg gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca
    51901 ggattcgcct gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc
    51961 caggcgtgat ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc
    52021 acgcccagcg cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga
    52081 tcttgcccaa caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc
    52141 gccagcgctg cacgccgcgc catcccacca gaaagttcag aaggcattag cttagccgct
    52201 ccacgcagcc ccacggcctc cagcttcatc atcactgtac tatgcaacaa tggcgcggga
    52261 agttgggtat gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac
    52321 aacgccccgg actgaaataa catgctcatc cgtttgcgca ctgtatacag gcgcgaacga
    52381 gacatcgccg gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc
    52441 ccgccaatca gacggagtag cgtcgtttta ccgatgcccg atggccccat gattgccgtg
    52501 atcttccctc gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta
    52561 aaactgacat cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg
    52621 tcttgcgttg attttctaag catggcgctc aatttaacct tgaacccaac atatttacag
    52681 aatattaccc gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag
    52741 tcaaaattaa gaatccgtta cggctttctg aaaatcttca gcggaccggc gagtatacct
    52801 gaagaaagga cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt
    52861 cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat
    52921 cccgccgctg atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat
    52981 cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg
    53041 ctcaaacatt atcaatatat tgctgatcct cggtctggct gcgctggttc gtccttttac
    53101 cgtccattct gatgttctac gccgtgaatt acccttaatg ttgttggtca gcgtggtggc
    53161 cggttccgta ctctatgacg gacaacttag tcgcagcgat ggtatctttc tcctctttct
    53221 ggctgtgcta tggctgctgt tcattgttaa acttgcacgt caggctgaac gtcaggggac
    53281 tgacagcctg accagagagc agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc
    53341 gtttttatgg ctcggcattg cgcttatcat catgccagtg gccacgcgga tggtggttga
    53401 taacgccacg gtgctggcga attactttgc catcagcgag ttgacgatgg ggctgacggc
    53461 aattgctatc ggaaccagcc tgccggaact ggcaaccgca atagcggggg ttcgcaaagg
    53521 tgaaaacgac attgctgtcg gaaatatcat tggcgcaaac atttttaata ttgtcatcgt
    53581 gttgggttta cccgcgctga taacgccagg agagattgat ccactggcgt acagtcgtga
    53641 ctacagcgtg atgttgctgg tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc
    53701 gcaaccgggc cgtggtgtag gggtattatt aactggcgga tttatcgtat ggctggcgat
    53761 gttgtactgg ttatcgccaa tactcgttga ataactggaa acgcattatg tcgcacgtag
    53821 agttacaacc gggttttgac tttcagcaag caggtaaaga agtcctggcg attgaacgtg
    53881 aatgcctggc ggagcttgag caatacatca atcagaattt cacgcttgct tgtgaaaaga
    53941 tgttctggtg taaagggaaa gttgtcgtca tggggatggg gaaatcgggg cacatcgggc
    54001 gcaaaatggc ggcaacgttt gccagcaccg gtacaccttc atttttcgtc catcctggtg
    54061 aagccgcgca tggtgattta ggcatggtta ccccacagga tgtggtgatt gctatctcta
    54121 actcaggtga atccagcgaa atcacggcct taattccagt gcttaagcgt cttcacgtac
    54181 cgttaatctg catcaccggt cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc
    54241 tgtgtgttaa agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc agcagcacca
    54301 ccgccacgct ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta
    54361 ctgctgaaga ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa cttctgctgc
    54421 gcgtaaacga tattatgcat acgggcgatg agatcccgca tgttaagaaa acggccagtc
    54481 tgcgtgacgc attgctggaa gttacccgca aaaatcttgg tatgactgtc atttgcgatg
    54541 acaatatgat gattgaaggc atctttaccg acggtgattt acgccgtgtc ttcgatatgg
    54601 gcgtggatgt tcgtcagtta agtattgccg atgtgatgac gccgggggga atacgtgtgc
    54661 gccctggcat tctggccgtt gaggcactga acttaatgca gtcccgccat atcacctccg
    54721 tgatggttgc cgatggcgac catttactcg gtgtgttaca tatgcatgat ttactgcgtg
    54781 caggcgtagt gtaaagattc aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc
    54841 gacctgttac ggccctgtca gcgccgacgt tatggcaaaa gcagagaaca ttcgtctgct
    54901 gatcctcgac gttgatggcg tactgtcaga tggcctgatt tatatgggca ataacggcga
    54961 agagctgaaa gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga
    55021 tattgaagtc gctatcatta ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac
    55081 attggggatc actcacttgt atcaggggca gtcaaacaaa ctgatcgcct ttagcgattt
    55141 gctggaaaaa ctggcgattg ccccggaaaa tgtggcttat gtcggcgatg atctcatcga
    55201 ctggccggta atggaaaaag tgggtttaag cgtcgccgtg gccgatgcgc atccactgtt
    55261 gatcccgcgc gccgattacg tgacgcgcat tgctggcggt cgtggcgcag tgcgcgaagt
    55321 ttgcgactta ttactcctgg cgcagggcaa actggatgaa gccaaagggc aatcgatatg
    55381 agtaaagcca gacgttgggt tatcattgtg ctatcactgg cggttctggt gatgatcggc
    55441 attaatatgg ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa tgatcccacc
    55501 tataaaagcg agcatacgga cacgctcgtc tataacccag aaggggcact aagctatcga
    55561 ttgattgctc aacacgttga atattattcc gatcaggccg tttcgtggtt tacgcagccg
    55621 gtacttacca cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc agataaagcc
    55681 aagctgacca atgaccggat gctctattta tatggacacg ttgaagtcaa cgcactcgtg
    55741 ccggactctc aacttcgcag aatcacgacg gataacgcgc agatcaatct ggtgacgcag
    55801 gatgttacct ctgaagacct cgtcacgtta tacggaacaa catttaactc cagcggtctg
    55861 aaaatgcgcg gcaacttacg cagcaagaac gccgagctga ttgaaaaggt tagaacatcc
    55921 tatgaaattc aaaacaaaca aactcagcct taatcttgtg cttgccagct cacttctggc
    55981 cgccagcatt ccggcatttg ccgtaaccgg agacactgat cagccgatcc acattgaatc
    56041 ggaccagcaa tctcttgata tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt
    56101 cacccagggc accatcaaaa ttaatgccga caaagtggtt gttacccgtc cgggcggcga
    56161 acaaggtaaa gaagtgattg acggctacgg taaaccggca acgttctacc agatgcagga
    56221 caacggtaaa ccggttgaag gtcacgcttc ccagatgcac tacgaactgg caaaagattt
    56281 tgtcgttctg acgggtaatg cttatctgca gcaggttgat agcaacatta agggcgataa
    56341 gatcacttac ctggtaaaag agcagaaaat gcaggctttc agcgacaaag gcaagcgcgt
    56401 aaccaccgtt ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc agaccccggc
    56461 acagaagaag ggtaattaat tcgttatggc aacattaact gcaaagaacc ttgcaaaagc
    56521 ctataaaggc cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg gggaaattgt
    56581 cggtctgctg gggccaaacg gtgccggtaa gaccaccact ttctacatgg ttgtaggcat
    56641 tgtgccgcgc gatgcgggca acatcattat tgatgatgac gatatcagtc tgctgcctct
    56701 gcatgcacgc gcgcgccgcg gtatcggcta tctgccacag gaagcctcca ttttccgtcg
    56761 cctcagcgtt tacgataacc tgatggcggt actgcaaatt cgtgacgact tgtctgctga
    56821 acaacgtgaa gaccgcgcga acgagctgat ggaagagttt cacattgagc acctgcgtga
    56881 cagcatgggg cagtcactct ccgggggtga acgtcgccgt gtagaaattg cccgcgcact
    56941 ggctgcgaat ccgaaattta ttctgctcga cgaaccgttt gccggggttg acccgatctc
    57001 ggttatcgac attaaacgca tcattgagca cctgcgcgac agcggcctgg gcgtgctgat
    57061 cactgaccac aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca
    57121 ggggcatttg atcgcccacg gcacgcctac agaaatctta caagacgaac acgttaagcg
    57181 tgtatacctt ggggaagact tcagactctg atagggtaga agtttgcgac gttttagcag
    57241 gagagtacga ttctgaacat gaagcaaggt ttgcaactca ggcttagcca acaactggcg
    57301 atgacgccac agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag
    57361 caggagctac agcaggcgct ggagagtaat ccgctgcttg agcaaatcga cactcatgaa
    57421 gaaatcgaca cccgcgaaac gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa
    57481 caaaaagaga tgccggaaga gctgccgctc gatgccagtt gggacaccat ttacaccgct
    57541 ggtacaccat ccggcaccag cggtgactac attgacgatg agctgccggt ctatcagggc
    57601 gaaacgacgc agaccttgca ggattacctg atgtggcagg tcgagctgac accgttttcc
    57661 gacactgacc gcgctattgc tacctctatc gtcgatgccg ttgatgacac cggttatctg
    57721 actgtcccgc tggaagatat tctcgaaagt atgggcgatg aagagatcga catcgacgag
    57781 gttgaagccg tccttaagcg gatccaacgg tttgatccgg tcggtgtagc ggcaaaagat
    57841 ctgcgtgact gcctgctaat ccaactctcc caattcgata aaaccacgcc gtggctggaa
    57901 gaggccagac tgatcattag cgatcatctc gatctgttag ccaatcacga cttccgcact
    57961 ttaatgcgcg tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa tctgatccag
    58021 tcgctcgatc cgcgccccgg gcagtcgatc cagactggcg aacctgagta tgtcattcca
    58081 gatgtgctgg tgcgtaagca taacggtcac tggacggtag aactcaacag tgacagcatt
    58141 ccgcgtctgc aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg caacgatgat
    58201 gacagccagt ttatccgcag caatctgcag gatgccaaat ggttgatcaa gagtctggaa
    58261 agccgtaacg atacgctact gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc
    58321 tttgagcaag gtgaagaata tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc
    58381 gaaatgcatg aatcgacgat atctcgcgtg accacgcaaa aatacctgca tagtccacga
    58441 ggcatttttg aactgaagta tttcttttcc agtcacgtca ataccgaggg cggcggcgaa
    58501 gcttcctcca cggcgattcg tgcactggtg aagaaattaa tcgcggcgga aaacccagcg
    58561 aaaccgttga gcgacagcaa gttaacctct ttactgtcgg aacaaggtat catggtggca
    58621 cgccgcactg ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa
    58681 cagctcgttt gacccaaccg ataaggaaga cactatgcag ctcaacatta ccggaaataa
    58741 cgtcgagatc accgaggcac tgcgcgaatt tgttacagct aaatttgcca aacttgagca
    58801 atattttgac cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag tcacccacac
    58861 ctcagatgca acactgcatg taaacggtgg tgaaattcat gccagcgcgg aaggtcagga
    58921 catgtacgct gccattgatg gcttaattga taagctggca cgccagttga ccaaacataa
    58981 agataaactg aaacaacact aattgtccgg gcaattagca tgtgcatggc ggcctgttgt
    59041 gcggcacaac gggccatttg tacggttaat gctccgagcc tgttccactg tttgagtggg
    59101 caggttctta ggtgaaatta tgacaaataa tgatacaact ctacagctta gcagtgttct
    59161 taacagggaa tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat
    59221 catcagcgag ctggcggcga aacaacttag cctgccgcct caggtggttt ttgaagctat
    59281 cctgacgcgg gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg
    59341 caaactggaa gaagatactc tgcgcgccgt tggcgttttc gttcagctcg aagcgcctat
    59401 agctttcgat gccatcgaca accaaccggt ggacttactt tttgccctgc tggtgccggc
    59461 agaccaaact aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa
    59521 aaccatctgc cgccgtttac gcgcagccca gagcgatgaa gagctgtatc aaatcattac
    59581 ggataccgaa ggtactccgg atgaagcgta gttattcggt aatgtctctt ttagacgttg
    59641 tgaggagaaa cagtacatgg tactgatgat cgtcagcgga cgttcaggtt caggtaaatc
    59701 tgtcgccctg cgtgcgctgg aagatatggg tttttactgc gtggataacc ttcccgtagt
    59761 gttgttaccc gatctggctc gaactctggc cgatcgcgag atttctgccg ccgtcagcat
    59821 tgatgttcgt aatatgccgg agtcaccaga aatattcgaa caggcgatga gtaacctgcc
    59881 tgacgctttc tcaccgcaac tactgttcct ggatgccgac cgtaatacct taattcgtcg
    59941 ttacagtgac acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc
    60001 tatcgacaaa gaaagcgatt tgctggagcc tctgcgttcg cgagcggatc tgattgtcga
    60061 tacctcagaa atgtccgttc acgagctggc tgaaatgctg cgtacccgtc tgctgggtaa
    60121 acgtgaacgc gaactgacca tggtctttga gtctttcggc ttcaaacacg gtatccctat
    60181 cgatgcagat tacgtctttg acgtgcgctt cttgccgaac ccgcactggg atccgaaact
    60241 gcgtccaatg acaggtcttg ataaacctgt cgccgcgttc ctcgaccgcc acacagaagt
    60301 acacaatttt atctaccaga cgcgaagcta tcttgaacta tggttaccta tgctggaaac
    60361 caacaaccgt agctacttga cggtcgccat tggttgtacc ggcgggaagc accgttcggt
    60421 gtatattgca gagcaactgg cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg
    60481 ccatcgtacg ctggaaaaac gtaaaccatg accgtcaagc aaactgttga aatcacaaac
    60541 aagctgggca tgcatgcccg gcctgcaatg aagctgtttg aattaatgca gggttttgac
    60601 gctgaagtgc tgttacgtaa tgacgaaggc accgaggccg aagccaacag cgttattgcg
    60661 ctgctgatgt tggattctgc caaaggacgg cagattgaag ttgaagcgac cggcccacag
    60721 gaagaggaag cactggccgc cgttatcgcc ctctttaact ctggttttga tgaagattaa
    60781 tcttcatcac tttgacatac aactcccttc aaactccccc cgacaataag aaaatcacgt
    60841 actgaaatcg ttctcaatca acgtcatttg tacattttgt gcgcttttca ctttcagaag
    60901 aaccttaaga aaaccttaag aggcattgtt taggttttgt ttaagttaat cgaccatact
    60961 ggagatcgtc agaaaatatt tccaggagat ggcatgattc gcttatctga acaaagtccc
    61021 ctgggcaccg gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc
    61081 aagattgtct accatcgtgg cgatggtggc gataaagaga tccgccgtga gttaaagtac
    61141 tacgcgcatc ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc
    61201 gttgagacgg attgcggaac cggttatgtc tacgatgtga ttgcagattt tgacggtaaa
    61261 ccctccatta cgctgaccga atttgctgaa caatgccgtt acgaagaaga cattgcacaa
    61321 ctgcgccagc tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg
    61381 tcgctgaagc cgcagaatat cctctgtcac cgcatcagtg agtcagaggt tatcccggtc
    61441 gtctgtgaca acattggtga aagcacattg attccactgg cgacctggtc gaagtggtgt
    61501 tgtctgcgta agcaagagcg gttatggaaa cgatttatcg cccaaccggc gctggctatt
    61561 gccctgcaga aagatctgca acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa
    61621 gcttaatcca gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt
    61681 aaaatccacg cctgacggct acgcacgtag cccgatggcg aggagacttt gaaacgaagt
    61741 ggattaggta atacagctgc cagtaatgca gcttccgacc gggtaagttt gctcgcgggt
    61801 ttgtggaaat aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg
    61861 atattcaggt aaaccgtcag gatacgcttt ttgctccaga ctgtttctat ccccagcgtt
    61921 aatccagctt ccagcccttt tcgtacccag ctacgcccat cccataaaaa gagatttttg
    61981 gccgtctgct gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg ctcgttgtgc
    62041 gccagggctt tctcaatgga agcgacatca aagccccagt gctcaggaaa tttctgatct
    62101 tctgcggcaa tcaccgccag tcccatccac ggcgagattt gatccatact gacccagtca
    62161 gaatgtgcca cgtaacgaaa attgccatgc agccaggcgc tgacctgtcg ctcgaccatt
    62221 accgctgaga agggaacagg cgcaacacta aacaacgcga tgcccccgcc ccagaaaacg
    62281 gcgaggacaa ccattaaccg caaaagaaaa cggcgaacga aactaaacac cgttaagcgg
    62341 cttttactca ttcagccaga accagcacgc gggaaaccag cttatcaatg ccgctcgccg
    62401 cttctgcaat gttctgcgcc agcatatatg ctggggtggt gacaatctta ttgtcttcat
    62461 caaccacgat atcatcgaca ggacagggca catgctccgc gcccatctct tccagcactt
    62521 ctgcggtatc gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa attttcggca
    62581 gcatcgccgg ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg
    62641 ccagcgcctt taattcacgg tcaacggtgc attcgctacc aagagtggca aaattgctta
    62701 aattcttcgc cgcgccaaac ccccccggca caatcaacgc atccagttca gcggcatcgg
    62761 cctgggccag aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc agcacgttgc
    62821 gcgtttccgt catcgcttcg ccagttaaat ggttgataac atcaacctgc tgcttatccg
    62881 gtgcaaagca gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt
    62941 catgaatttc agaaccgtca tagacgccgc atccgctcag aattacgcca attttcttca
    63001 ttgtgatcat cctttcgcaa ttgactgaaa cacattaatt tttttaataa aaatggtacg
    63061 catcacacat ttaactgatt catgtaacaa atcatttaag ttttgctatc ttaactgcgt
    63121 gcggcctgaa aaacagtgct gtgcccttgt aactcatcat aataatttac ggcgcagcca
    63181 agatttccct ggtgttggcg cagtattcgc gcaccccggt ctagccgggg tcatttttta
    63241 gtggcttttg ccacccacgc tttcagcact tctacgtcgt gacgccactc ttctttcatc
    63301 tcttcaatcc attcaccgac gttatcttcc caggcaggaa ggtccggaga ctgaatttgc
    63361 tgacccagct gttgcagatg gcgtaacccc actgaccccg ccgcaccttt aattttatgt
    63421 ccttcctcta caatgccttt tttatcctgc gccgtcagat tcgactccag cacgctgaca
    63481 tagcccggca tcatcttctc aaacaccgct aacccgtcgg tgatcagctt cggtcctaca
    63541 agttcgagat actgttccag catgggaata tcgagcaatg cttctgattt actgttctct
    63601 tctgtcgtca ccgtactctc ctcatcatcc tgggtatccc agaatttctt gatcatcgcg
    63661 gttagcgccg gaaccgaaag cggcttactc agcacatcat ccattccagc attgaggtac
    63721 tcttgtttgt ctttcagcac gttagcggtt aaggccacca gcggcggtaa atcctcgcgc
    63781 ggataacgct tcgtcagttc acgagagata tccagcccgg tcatatctgg caactgaata
    63841 tccagcaaca ccaggtcgta ttcgcccggt ttaaacatct ccagcgccgc cttgccggtc
    63901 atggcgacat caacgctgtt acctaatttt tccagcacag aacgcgcaac aatcacgttc
    63961 agttcaatgt cttccaccag cagcacattc agcgccggta aaggcatatc gtcttcatca
    64021 aacgcatcat cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa
    64081 cctttgccct gttcgctggt aaccgtaata tcgccgccca tatttttcgc cagacgacga
    64141 gaaacggcca gaccaatacc ggtgccggtg gcaggtttac cgccatgact gtctttcacc
    64201 tggtaataca tggcgaaaat tttatccagc tcatcctgcg gaatgccgat accagagtct
    64261 tccacttcaa aatgcagcat atcgccttca tcgtagcgca cgcgcacggt aacctggcct
    64321 tgctgggtga atttgacggc gttactgatg aggttccaca ggatctgccg taaacgcgtc
    64381 ccgtcggtaa tgacctgatg cggtaatggc agcgtcggct ccaggttaaa gcacaatcct
    64441 ttttgttgcg cctgcaatgc ggagagattt tccagatcgg caaggaagct ggtgaaatca
    64501 accggttgat tatcaagctg gaccttgcgc cgttccatct tatccatgtc gataatatcg
    64561 ttaaagatat tccccagcgt gacggccgaa acatggatgg tcttgagata tttttcctgc
    64621 tcggcggtga gttcggtatc cagcagaata cggctcaggc cgacgatacc gttcagcggt
    64681 gtacgcaatt cgtgactgat ggtggaaata aacgtcgttt tgtcgcggct ggcccgttca
    64741 agcgcatcct gataccgctt acgctcggta atgtcgcgac caaagcccat caaaccgtga
    64801 cgtttaccca cgcggtcgta gtacggcact ttacggattt caaagcaggc tttgcgcccg
    64861 tccgggtaat ccagccactg ttcataggtc agtgacacat tatgacggaa cactttttca
    64921 tcggtttcaa tgacttttgc ggcggcttcc ggtgagtaaa catcagcagg tttcaggtga
    64981 accagttgtt tttcgctttt tccggtcagc agctccatcg cgcggttaca gccggaaaac
    65041 tctttatctt cgttacgata aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaag
    65101 aatgaggatt gctgctcgag ctgaatttgt gtctcttcgc gctctttgat ttcaattttc
    65161 agttggccga aggtttcctg cagttctgct tccgcttttt cacgaacggc aatttcctga
    65221 tttagctggg caatattatc ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc
    65281 tcctccagtt tttgcaccag ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg
    65341 accaccgata gaaagtagac cgcccacggc gtaatcagca aaccaaagaa gatagaacga
    65401 ataacatcaa tgctttcgac ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca
    65461 atggcaagaa cgacgagggc cagcgccagc aacattgaga agcgcaccag acctaacttc
    65521 atcatcaggt caacataata ctgcgccagc agacgaattt gcttcattag ggaattcctt
    65581 cacgacaacc tgccccaata atacccaatt ctgacagtta cgttataaat tgtgtgagaa
    65641 atgcggaatt cccctcaacg acctactccg ttggaggtat ccacggacgt tccagcgccg
    65701 atccctgtac gccatgttca ttcagatagc gatccagctc gaccattccc gtccatcgat
    65761 tttcgcacca cagaggagca agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt
    65821 agatcacttc cggtggcgta tggcgaatca tttctccggc agtgagcgtg taatcctcca
    65881 gttcaatacc gttcaaacgc cccgcttccc aggctttcgc cataatgctg cctttcacaa
    65941 tatgcagcgg atgcagcttt atgccgtcca cgccggtttc aaccactcgc tccagcgttt
    66001 gcaaacattc agcctgccct tcgccaggca ggccgacaat taagtgggaa cacaccttca
    66061 gcccgcgctg acgtgccagc tgggttgtac gctgataaca ggcaaaatca tggccgcggt
    66121 tgatgcgatg cagtgttttg tcgtgggcgg tttgtagccc cagctccagc cacacttcgt
    66181 aaccctggtc cttatattcg caaagcagat ccagcaccgc atccggcacg cagtccgggc
    66241 gggtaccaac acacaaaccg acaatattgg cctggctcac cgcctgctga tacatagaac
    66301 gcagcacctg aacttccgca aaggtgctgg tatacgcctg aaagtaggcc agatagcgtt
    66361 tagcgcggtt aactaaattc gcctggtgcg ccagttgctc ggcaatggaa cgatgctgct
    66421 gcgcttcatc ggcaaacgag gcaacattac agaatgtgca gccaccacgc ccgatggtac
    66481 cgtcacggtt agggcagcta aaaccgccgt gtagcgtcag cttatgcacc ttttgcccat
    66541 aacgacgggt gagatcacca ccaaacatat tgactaattt ctgtaactgc ataatctgat
    66601 agaccgcgcc ttgaaaagag gccaaagcct gccattttta gctcaattcg gcgatgacct
    66661 ggatcaatcg tcccggcctg cttttatcaa ctgcataatc aatcaaaatt accgaaattt
    66721 catgcataat cacataaatc acttttgctt atcttgtgtc agattttttt atctccttat
    66781 ggattttagg caaaaacagt agcatgaaac gtcattacca attaaggcag tataaaatgc
    66841 tggttttctc gtcagttcaa ggcaggataa gggttaacac gcctttatga cagtcaggaa
    66901 ttgactgttt ctctaacgac ttccctttta gccttaaaga taaaatccat tttaatttca
    66961 gtaatttaat aaagaatttt gcgctaaagc acatttctgt accaataagc ttgccatttg
    67021 acctgtatca gctttcccga taagttggaa atccgctgga agctttctgg atgagcagcc
    67081 tgctcatcat atttatgcag taattgagat cccctcttca ccgtattaac cgatgcgaaa
    67141 aggacaacaa gggggcgaat gcgaggcgcg cgtatgacac gcaaaccccg tcgccacgct
    67201 ctttctgtgc ccgtgcgcag cggttcggaa gtggggttcc cgcagagcct gggggaggtt
    67261 cacgatatgt tgtacgataa atcccttgag agggataact gtggtttcgg cctgatcgcc
    67321 cacatagaag gcgaacctag ccacaaggta gtgcgtactg caatacacgc actggcccgc
    67381 atgcagcacc gtggcgcgat tctcgccgat ggtaaaaccg gcgacggttg cggcttgctg
    67441 ttacaaaaac cggatcgctt ttttcgcatc gttgcgcagg agcgcggctg gcgtttagca
    67501 aaaaactacg ccgtcgggat gctattcctg aataaagatc ctgaactcgc cgctgccgca
    67561 cgccgcatcg ttgaagaaga actgcaacgc gaaaccttgt cgattgtggg ctggcgtgat
    67621 gtccccacta acgaaggcgt gctgggtgaa atcgccctct cctctctgcc acgcattgag
    67681 caaatttttg tgaacgcccc ggcaggctgg cgtccgcgcg atatggagcg ccgtctgttt
    67741 atcgcccgcc gccgcattga aaagcgtctc gaagccgaca aagacttcta cgtctgtagc
    67801 ctgtcgaatc tggtgaacat ctataaaggt ctgtgtatgc cggcggatct gccgcgcttt
    67861 tatctggatc ttgcggacct gcgtctggaa tcggccatct gcctgttcca ccagcgcttc
    67921 tccactaaca ccgtaccgcg ctggccgctg gcgcagccgt tccgctacct ggcgcataac
    67981 ggtgaaatca acaccatcac cggtaaccgc caatgggcgc gtgcgcgtac ctataaattc
    68041 cagacgccgc ttatccctga tctgcacgac gccgcaccgt tcgtcaatga aaccggctct
    68101 gactccagtt cgatggataa catgctggaa ctgctgctgg caggcggaat ggatatcatc
    68161 cgcgccatgc gtctgttagt gccacccgcc tggcagaaca acccggatat ggacccggaa
    68221 ctgcgtgcct tctttgactt taactccatg catatggagc cgtgggatgg cccggcgggc
    68281 atcgtgatgt ccgacggtcg ttttgccgcc tgtaacctcg accgtaacgg tctgcgtccg
    68341 gcgcgctacg tcatcaccaa agataagctc atcacctgcg cctctgaagt cggtatctgg
    68401 gattaccagc ctgacgaagt ggtcgaaaaa ggccgcgtcg ggccaggcga actgatggtt
    68461 atcgacaccc gcagtgggcg tattctgcac tcggcagaaa ccgatgacga tctgaaaagc
    68521 cgccatccat ataaagagtg gatggagaaa aacgtccgcc gactggtacc gtttgaagat
    68581 ctgcccgatg aagaagtggg tagccgcgaa ctggacgacg acacgctcgc cagctatcag
    68641 aaacagttta actacagcgc ggaagagctg gactccgtaa ttcgcgtact gggcgaaaac
    68701 ggtcaggaag cggtcggttc gatgggcgac gacaccccat tcgccgtgct ctccagtcag
    68761 ccgcgcatta tttacgacta cttccgccag cagtttgccc aggtgactaa cccgccaatc
    68821 gacccgctgc gtgaagcgca tgttatgtcg ctcgccacca gtatcggtcg tgaaatgaac
    68881 gtcttttgcg aagcagaggg ccaggcgcac cgtttaagct ttaaatcgcc gattctgctc
    68941 tactccgatt tcaaacagct cacgacgatg aaagaggagc actaccgcgc agatacgctg
    69001 gatatcacct ttgacgtcac taaaaccacg ctcgaagcga cagtcaaaga gctgtgcgac
    69061 aaagccgaaa aaatggtacg tagcggcacc gtgctgctgg tgctctccga ccggaatatc
    69121 gctaaagatc gcctgccggt tccagccccg atggcggttg gcgcgatcca gacccgtctg
    69181 gtcgatcaaa gcctgcgttg cgatgccaac atcatcgtcg aaaccgccag cgcccgcgat
    69241 ccgcaccact tcgccgtgtt gctgggcttc ggcgcgacgg ctatttatcc ataccttgcc
    69301 tatgaaacgc tgggccgcct ggtagacacc catgcgattg ccaaagatta tcgtaccgtg
    69361 atgctcaact accgtaacgg catcaacaaa ggcttgtaca aaatcatgtc caaaatgggc
    69421 atctccacca tcgcctctta ccgctgctcg aaactgtttg aagcggtcgg tctgcacgat
    69481 gatgtagtgg gcctgtgctt ccagggggcg gtcagccgca ttggtggggc gagctttgaa
    69541 gacttccagc aggatctgct gaacctgtcg aaacgtgcct ggctggcgcg taagcccatc
    69601 agccagggcg gcctgctgaa atacgtccac ggcggcgaat accacgctta caacccggac
    69661 gtggtgcgca cgctgcaaca ggcggtacaa agcggcgagt acagcgacta tcaggaatac
    69721 gcgaagctgg ttaatgagcg tccggcaacc acgctgcgcg atctgctggc aattacgccg
    69781 ggtgaaaacg cggtcaacat tgctgatgtt gaaccggcaa gcgaactgtt taaacgcttt
    69841 gataccgccg cgatgtctat cggcgcgtta agcccggaag cccacgaggc gctggcggaa
    69901 gcgatgaata gcatcggcgg taactcgaac tccggtgaag gcggcgaaga cccggcgcgc
    69961 tatggcacta acaaagtgtc gcgcatcaag caggtggctt ctggtcgctt cggcgttact
    70021 ccggcgtatc tggtcaatgc cgacgtcatt cagattaaag tcgcccaggg cgcgaagccg
    70081 ggcgaaggcg gtcagttgcc gggcgacaaa gtcactcctt acatcgccaa actgcgctat
    70141 tcggtgcccg gagtgacgct gatctccccg ccgccgcacc acgatatcta ctctatcgag
    70201 gacttagcgc agctcatttt cgacctcaag caggttaacc cgaaagcgat gatctccgtg
    70261 aagctggttt ccgaaccggg cgtaggcacc atcgcgactg gcgtggcaaa agcttatgcc
    70321 gatttaatca ccatcgcagg ctatgacggt ggcaccggcg caagcccgct gtcgtcagtg
    70381 aaatacgcag gttgcccgtg ggagctgggg cttgttgaaa cccagcaggc gctggttgcc
    70441 aatggtctgc gtcataagat ccgtttgcag gtcgatggcg gcctgaaaac cggtgttgat
    70501 atcatcaaag cggcgattct cggcgcagaa agcttcggct tcggcactgg cccgatggtg
    70561 gcgctcggct gtaaatatct acgtatttgc catctgaaca actgcgcaac gggtgtagca
    70621 actcaggatg acaaactgcg taagaaccac tatcacggcc tgccgttcaa ggttaccaat
    70681 tactttgaat ttattgcccg tgaaacccgc gagctgatgg cacagctggg cgtaacgcgt
    70741 ctggtggatc tgattggtcg caccgacctg ctgaaagaac tggacggttt caccgccaaa
    70801 cagcaaaaac tggcgctgtc gaagctgctg gagaccgccg aaccgcatcc aggtaaggca
    70861 ctctactgca ccgaaaacaa cccaccgttt gataacggcc tgctgaacgc gcagttgctg
    70921 caacaggcga aaccgtttgt cgatgaacgc cagagcaaaa ccttctggtt cgatattcgc
    70981 aacaccgacc gttctgtcgg cgcgtcgctt tcaggctata tcgcccagac gcacggcgat
    71041 caggggctgg cagccgatcc tatcaaagcg tacttcaacg gcaccgcagg ccagagtttc
    71101 ggtgtgtgga acgcgggcgg cgtggaactg tacctgaccg gggatgccaa cgactatgtc
    71161 ggtaaaggca tggcgggcgg cttaatcgcc attcgtcctc cggttggttc cgccttccgc
    71221 agccatgaag caagtattat cggcaacacc tgcctgtatg gcgcgaccgg tggtcgtctg
    71281 tatgccgcag gccgcgcggg tgaacgtttc ggcgtgcgga actccggtgc tatcaccgtg
    71341 gtagaaggca ttggcgacaa cggctgtgaa tatatgacgg gcggtattgt ctgtattctg
    71401 ggtaaaaccg gcgttaactt cggtgcgggc atgaccggcg gcttcgctta cgttctcgat
    71461 gaaagcggtg atttccgcaa acgcgttaac ccggaactgg tcgaggtctt aagcgttgac
    71521 gatctggcga tccatgaaga gcatctgcgc ggtcttatca ccgagcatgt tcagcatact
    71581 ggctctcagc gcggtgaaga gattctggcg aactggtcaa ccttcgccac taaatttgcg
    71641 ctggttaaac cgaagtccag tgatgtaaaa gcactgctgg gtcatcgtag tcgtagcgca
    71701 gcagagttgc gcgtgcaggc gcagtaaggg gtagcaacaa tgagtcagaa tgtttatcaa
    71761 tttatcgacc tgcagcgcgt tgatccgcca aagaaaccgc tgaagatccg caaaattgag
    71821 tttgttgaaa tttacgagcc gttttccgaa ggccaggcca aagcgcaggc tgaccgctgc
    71881 ctgtcgtgcg gcaacccata ctgcgagtgg aaatgtccgg tacacaacta catcccgaac
    71941 tggctgaagc tcgccaacga ggggcgtatt tttgaagcgg cggaactgtc gcaccagacc
    72001 aacaccctgc cggaagtttg cggacgagtc tgcccgcaag accgtctgtg cgaaggttcc
    72061 tgcactctga acgatgagtt tggcgcggtg accatcggca acattgagcg ttatatcaat
    72121 gataaagcgt tcgaaatggg ctggcgtccg gatatgtccg gcgtgaagca aaccggtaaa
    72181 aaagtggcga ttatcggcgc aggcccggca ggtctggcgt gtgcggatgt cctgacgcgt
    72241 aacggcgtaa aagccgttgt cttcgaccgt catccagaaa ttggcgggct gctgaccttc
    72301 ggtattccgg ccttcaagct ggaaaaagag gtaatgacgc gtcgccgtga aatcttcact
    72361 ggcatgggta ttgaattcaa actcaatacc gaagtgggcc gcgacgtgca gctggacgat
    72421 ctgctgagtg attacgatgc cgtcttcctt ggcgtcggga cttatcagtc aatgcgcggc
    72481 gggctggaaa acgaagacgc cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac
    72541 accaaacagt taatgggctt tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc
    72601 aaacgcgtgg tggtccttgg cggtggcgac actgcgatgg actgcgtgcg tacgtccgtg
    72661 cgccagggag cgaagcacgt tacctgtgcc tatcgtcgtg atgaagataa catgccgggt
    72721 tcccgccgcg aagtgaaaaa cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag
    72781 ccactgggga ttgaagtgaa cggtaacggt aaagtcagcg gcgtaaaaat ggtacgtacc
    72841 gaaatgggcg aaccggatgc caaaggccgt cgccgcgcgg agatcgttgc aggttccgaa
    72901 catatcgttc cggcagatgc agtgatcatg gcgtttggtt tccgtccaca caacatggaa
    72961 tggctggcaa aacacagcgt cgagctggat tcacaaggcc gcatcatcgc cccggaaggc
    73021 agcgacaacg ccttccagac cagcaacccg aaaatctttg ctggcggcga tatcgtccgt
    73081 ggttccgatc tggtggtaac cgctattgcc gaaggtcgta aggcggcaga cggtattatg
    73141 aactggctgg aagtttaagc tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg
    73201 aaattctcct ttctttctgg aaagcgcctc gcataatctg aagaaaacca atgtaatacg
    73261 ctcatctgga ttgttaccgg tagatgcccg gcgtatagct gattccatta ttcaacgcag
    73321 cgccatataa agggacagat aatggaatcg ctctcagaga gaacctcaac aggctaccag
    73381 caaatccacg acggtattat tcatctggtc gatagcgccc ggacggaaac ggtacgtagc
    73441 gttaacgcgc tgatgaccgc gacatactgg gaaattggcc gacgaattgt cgaatttgaa
    73501 caaggtggcg aggccagggc tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat
    73561 ttaagtctaa ggtataagcg tgggttctct gcaaaaaact tacgccaaat gaggcttttt
    73621 tacctctttt ttcaacatgt tgaaattcgc cagacagtgt ctggcgaatt aacaccattg
    73681 ggaattccgc agacaccatc tgcagaattc ccatcggcaa agatttggca gacgctgtct
    73741 gccaaatctt tccccctgcc ctggtccacc tacgtccgtt tactttccgt taaaaacgct
    73801 gacgcccgca gcttttatga aaaagagacg ctccgctgtg gctggtctgt tcgccagcta
    73861 gaacggcaaa ttgcgaccca attttatgag cgaacactac tgtcacatga caaatcagcc
    73921 atgctgcaac aacacgctcc taccgagacg catattcttc cgcagcaggc gatacgcgat
    73981 ccctttgtgc tcgaatttct ggaattgaaa gatgaatact cagaatccga ttttgaggag
    74041 gcgctgatca accacctgat ggatttcatg ctggaacttg gggatgattt tgcctttgtt
    74101 ggtcgacagc gaaggttacg cattgatgac aactggtttc gtgtcgatct gctgtttttc
    74161 caccgccgtt tacgctgcct actaatcgtc gatctaaaag tgggcaaatt cagctatagc
    74221 gatgccggac agatgaatat gtatctcaac tacgccaaag agcactggac gctaccggat
    74281 gaaaatccgc ccatcggtct ggttctctgt gcagagaaag gagccggaga agcacattat
    74341 gctctggcag gtttgcctaa caccgttctg gcaagcgaat ataagatgca actacctgat
    74401 gagaaacgac tcgcagatga actcgttcga acacaggcgg tgctagagga aggctataga
    74461 cgccgttaat ttcagcggtt tcgaattctg cagacagtgt ctgcagaatc tttaaaacga
    74521 actaagtttt aagccattaa atcagcctta accttcacca caaccttttt aatctctcca
    74581 ggctcgccga caacgcatcc tggtttatgc ggttcacctg gcataaacac ggcgaacatt
    74641 cccggtttta agatgatggt ttgctcgttt tcaatggtgc tgcaaagctg ataatcatcc
    74701 tcatggtgga actcttcaca ctggcgcgca gtgcctgcca tgccaaacag aatccgttcc
    74761 tcaccgttta ataacagttg gatatcaatg tattgctcgt gcaattccgc ttttttctcg
    74821 acgggcgatt gagtgttaaa cgtcatgaca ttcataaaga tattgtcgcc ctgtaattcg
    74881 taacgacccg gcgctttttc ttgcggcctg gcggctaatg ccagcgttaa cgcgtcctgt
    74941 aacgcaggat gtaacccagc agacggtaat gactgtactt cacccatcat cataattttt
    75001 ctccctgggc caacagcgca gccccaagta aacctgcatc atggcggtag tgcgccgcca
    75061 gtaaatcaac atgaaatgcc gctggctcct gcgccagata cgtttccacc agcgccagat
    75121 acccttctgc cagaccaacg ctgccaccga ccaccacgca ctggcaatca gttgtggctt
    75181 taatatcagc gatcagcctt gcaagcgtac gtgcggagcg atggatcagc cattgcgctt
    75241 gttcgtcgcc ctgcccggcg cgtgtaaaaa tagttctcgc atccgcgccc gccagttcac
    75301 cctgcgctgc cgccgcaatg ccgcgaccag aagcaatcgc ttccacgcaa cctgtgcgtc
    75361 cacagccgca gactggacca tgcggatcgg caagcgtatg cccgatatgc cccgccagac
    75421 cgccagggcc ggtaagcagt ttaccgccgc tcactacacc gccgccgacg ccagtggaaa
    75481 cggtgataaa caccatctcg gttacatcgc cttccagcgc ctgatactct gcccatgctg
    75541 cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt aagttgttcc agcgttttga
    75601 ctaacggaaa gtgtagcaat ccaccgagat tatgcggatt cagcgccagc aaactgccgt
    75661 cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc atgagcttgc aacggagaga
    75721 ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt
    75781 cacgacgatc gcggatctgc ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc
    75841 cgatatcaat cgccagtgtg gtcatagcac cgcctttttc atcgctgtgt tgtaccactg
    75901 acaaatgtgt tcaagacgcg taatggcaga accgaccgtc accgcccacg cgccgtggcg
    75961 catcgcatct gccgcctgag caggcgtgtt gtaacgccct tcggcaatca cccgacatcc
    76021 ggcgtcgctc aacgttttca ccagcgccag atccggctct tctggcgttt caggcgtggt
    76081 atagccagaa agcgtagtgc caataatttc ggctcccagc ttttggcatg ccaggccgtc
    76141 ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga tggtgaatac gtgccagcag
    76201 cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc
    76261 cgcctgcgcc agcgcatcga catcttcaat ataggccgtg atgcgtaccg gagaatcctc
    76321 cagatcgcgt ttcacaattc caataatcgg cacgctcacc accgcacgcg tggcttgcag
    76381 atttgccaca ccttcaatac gaatggcgac cgcgcccgcc tgctctgccg ctaatgccat
    76441 ggcggcgacg atttcgggtt tatcgagcgg gctgtcagga accggctggc aggagacaat
    76501 caggccaccg ttagcagcga ttttttgatc cagttgtgca agtaacgaca tacatcttcc
    76561 cttagcgaaa ggcccggtac agagaccggg caacaggatt aacttttggt tttgactaaa
    76621 tcgtttttgg cgctgccaaa cggcacggca ccgctgaatg gtttaccgtc gatagcgtca
    76681 tgagtacgca acgcttccgg gcgcaaccaa cgctgaacgc gagaaggcat atccagccca
    76741 atcagcagga tcaccacgaa cgtcagactg aacgagagcg atgccagcgc agtacccaga
    76801 tccagacgtt gagcgatcaa cgcgccgatg attggggcca gtgcaccgcc caatgcgcca
    76861 acgttgtagg taaagcccag gcccgctgca cgctggtcgg tatcgaaata accgccaatc
    76921 agttttggta agatcccggc gatcccttgt ccaagcattt gctggaagaa cagtaacaga
    76981 ccgagcaccc agacgtttgc gccgccaatc gcaaataccg gaataatcag cagctgcgag
    77041 gccagcaggc tacaaacgta cgctttgcgg gttcccagcc agtcaccgag gaagccacct
    77101 acgcagcatc ccaccgccgc gccaaagcca ctaaagaaca gcacattggc tacagtatgc
    77161 gggttataag ccagatcggt tttcagatac gttggcagca gcgcctgaat cggccatgag
    77221 tagaggaaag caaacaacac gaccaccatc agcattacgc ccgttggcca gcgtttgcct
    77281 gtactctgca ccataaagct gataaagatt gcggcgcata acagcccaag aacagcgacg
    77341 atcgcggcat tttgcaggtt accggcgaag cagaaccaca gcgcagtagc cgccgccagt
    77401 gtcattacga tattggcaat gcgatgttca ccacggtaga gaatatccac cattgtgcgt
    77461 actggtgctt tacctgcgtg tttctctttc cagtcttccg cttccgggat gtttttacgc
    77521 agccagagag caaagatgat tggcaaaatg ccgataaaga acagcgcacg ccagccccag
    77581 accggaacca ccaggctata gacctgagcg gcaacgacgg cccccacaga gaagcctgaa
    77641 atcagaaaac cactggcttt gttacgcaga tgttttggcc agctttcaat gacataggtg
    77701 gcgctggaac cgtattcacc cgccatcccc atgccgatga ccagacgcgc gataaacatg
    77761 gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg
    77821 gtgaccattg ccagacgacg cccgtagcgg tcacccatag cgccgagcat caggccgccg
    77881 aaccagcgag agataaaggc tgcggagatc agacttgccg cctgcaccgt cgtcagcccg
    77941 aattcaccct gtacttcggt gagtaccagg gcgattaaaa cgaaatcaaa accgtcaagc
    78001 agatatccca accaggcagc ggaaaatgcg cgccattgtg cacggttgag atggcgatac
    78061 cacgggatat tctgggttgt agtactcatt gtgagtctcc cgcggtgggc gatgcccaca
    78121 cgctttggta taaaaattgt agggtacaga tgcgtttatt tcccctcacc cggtaggggc
    78181 gagcgagggg aaacaactca cccgcgctct tgcatcaact gctgggccag cgccttcagt
    78241 tctggcagat atttttcatc taccggtcca aacggtttgc ggcacagcgg cacagaaacg
    78301 acatccatat aatggaggac agttttcagg ccgcggaata cgcccgtttt gatcagtaaa
    78361 tcaatgactt tattgcattc agtttgcagt ttctgcgcgg tctggatatc gccttctttc
    78421 agcgccttaa cgatcccctg atagcgccag cccatgatgt tgtaggtact accgatacca
    78481 ccatcagcgc ccgccagcag accagaggcg aagatttcgt cgtaaccgtt atagagcaca
    78541 agatcaggat gttcacgacg gatctgctcc atctgataga gatcgccaga ggtctgtttc
    78601 agcgcaccta cgccaggcaa tgtaacaagt gtgttgatct gatccagggt cagttttacc
    78661 ccactcaggg ctggaatgtt gtacaccacc atcggcaaac catccgccga atcaataatt
    78721 gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg
    78781 gagacggcat cgaagccata acgtttagcc gatgccgcaa gttgttggct ttcggcggtg
    78841 ctgacgcaac cgacgtgggc gatgagttta atcttacctt tcgcctcttc ggcgacgatt
    78901 tccagtacct gttcacgctc ggaaaggctt tgtacaaagg cctcgccggt cgaaccaccc
    78961 acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa ccaggcgacg cagactcgct
    79021 ttatccagtg cttgttgttg gtcaaaagga gtcaggagtg cagccattac gccacgtaaa
    79081 ttcgttgcca taaatacctc tgaagtgatg cttgtctgat aaacgatata cctttatacc
    79141 tgttatacca gatcaattaa gcaacacccc atacagaaag cttataatgc gatctgcttc
    79201 actaaagtgg cattatttct ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga
    79261 gacgctgttg agatgcgatt gcaacgcacg atcggcttcg tcaggatcat gacggcggat
    79321 cgcatcaacg atcgcaatat gctgttgata actaacgttg ttatgttcgt gcagtgcctg
    79381 atcggtaacc gttgggcgtg cggcaataag ccagtcgagc agggcaacgt ggatcgccat
    79441 gaagattggg ttaccgggga tctccgccag cacgcggtgg aaatcaacgt ctgaacgaat
    79501 gaatgccgcg ttgttatcca gcgactgact gttgatttcc agtgcttttg ccagcaaatc
    79561 gatttgctca tcggtggcat gttcagccgc atagcgcacc aggctggatt caaagaacag
    79621 acgtaattgt tcgaaatggg caatcccacc aggatgagaa aggaaatctt tcgccatgcc
    79681 ggaaagctcg ccgatgatgg tgtccgcaga aggacgtgag acgcgggcac gttcgccgtt
    79741 gttgatttgc accagacctt tgcgttttaa cgctgccagc gcttcgcgca cagaaggacg
    79801 cccgacgtta aagaacgcca tcagttcgcg ttcagacggt aattgttcac cttcgccaaa
    79861 ttcacgacgg cggatcatct gttccagctc ttcttccacc atttcggaga gttttttacg
    79921 cgccagcggg cggctacgca agttgcgacc aattgcaggt gaagaatctt cggtttgcga
    79981 atcaaatgcg ttcataaggc ccattctgta aggtcagtgt gattaacatc atcagtgaca
    80041 tcctatcaca ggattgaaag taggggaaaa tggcaggttt ttctctttgt gcctcatcat
    80101 taccataatt aacggaataa ttaactattg cgaaaaatta atgtaacgca gataaaaaca
    80161 tcccgtttga attatttata agactattca cgagcattat gaatattatg aatgtgttct
    80221 tacaaaataa tcataagcgc atatttttta atgaaaaatc acctcaccta caattaaaaa
    80281 cacgacatcc gcaccataaa tagacttgca aaaaatataa catcattgtt ttcaatctgc
    80341 cgtttatggg attgaccgtt ttcttttgac acggagttca acaatgttcg gcataattat
    80401 atctgtcatc gtattaatta cgatgggcta tttgatcctg aaaaactaca aacctcaggt
    80461 ggtgctggct gccgcaggta tcttcctgat gatgtgcggt gtctggttag ggttcggtgg
    80521 tgtactcgat cccgccaaaa gcagcggcta cttgatcgtc gatatttata atgaaatcct
    80581 gcgcatgctg tccaaccgca ttgccggatt ggggctgtcg attatggcgg tgggcggtta
    80641 tgcccgctac atggagcgca caggagccag tcgcgcgatg gtgagcttgc taagccgccc
    80701 gttaaaactc attcgctcgc cgtatattat tctgtcggca acttacgtca tcggccaaat
    80761 catggcgcag tttattacca gcgcctccgg cctgggtatg ttgctgatgg tcaccttatt
    80821 tccgacgctg gtgagtctgg gagtaagtcg cctctctgcg gtggcggtta tcgcaaccac
    80881 gatgtccatt gagtggggga ttctggaaac gaactccatt tttgcagccc aggtcgcggg
    80941 aatgaaaatt gccacttact tcttccacta ccagcttccg gtcgcctctt gcgtcattat
    81001 ctcggtggcg atctcccact ttttcgtgca acgcgctttt gacaaaaaag ataaaaatat
    81061 caatcacgaa caggcagagc taaaagctct cgataatgtc ccgccgctct attacgccat
    81121 tttacctgtg atgccgttaa tcttgatgct cggctcgctg ttcctcgccc acatcgggct
    81181 gatgcagtca gaactgcatc tggtggtggt gatgttactg agtttgacgg tgacgatgtt
    81241 tgttgagttc ttccgcaagc ataacttgcg cgaaacaatg gacgatgtgc aggcgttttt
    81301 tgacggcatg ggtacgcagt ttgccaacgt ggtaacgctg gtggtcgcgg gtgaaatatt
    81361 tgcgaaaggc ttaacgacga ttggcactgt tgatgcggtt atcaggggtg ctgagcattc
    81421 tggtctgggc ggtattggcg tgatgattat tatggcgcta gtcattgcca tttgtgccat
    81481 tgtgatgggc tctggcaatg cgccgtttat gtcatttgcc agtcttattc cgaatatcgc
    81541 agccggacta catgtaccag cggttgtaat gattatgccg atgcattttg ccacgacgct
    81601 agcgcgcgcg gtttcgccga ttactgcggt ggtggtcgtt acgtcaggaa ttgcaggcgt
    81661 ttcgcctttt gcggtggtga agcgaacagc gatccccatg gcagtcggtt tcgtggtgaa
    81721 tatgattgcc acaatcacgc tattttatta agccattaaa agacaaaaca ggccaccagg
    81781 gcctgttttt gtattacttc acaacgcgta atgccggtcg accaccgcgt ggtggctgcg
    81841 gaggttcatc gtcaggatga gtgtcatcat cgtgatctgg cttgtcgcca tcaataaccg
    81901 acataacggt ttcgttgtct gccgatgcct cttcatcatt catgatgctg gtatcttcat
    81961 cgtaggcagc ttcaggctca aacatcgtgc ctgcgccatt ttcacgggcg tagatagcca
    82021 gcacggcagc cagcggcaca gaaacctgac gcggaatgcc gccaaagcgc gcgttaaagc
    82081 gtacctcatc attcgccagt tccagattgc cgacagcacg cggcgcaatg ttgagtacga
    82141 tttgcccgtc acgcgcatat tccataggaa cctgcacgcc agggagcgtc acatccacca
    82201 ccaagtgcgg cgtgagctgg ttatccagca accactcata gaatgcacgc agcagatagg
    82261 gacgacgtgg tgttagctgt gacaaatcca tacagattaa ctccggccca gacgcatttc
    82321 acgttctgct tcagttaaag aagcaaggaa agagtcacgc tcaaagacgc gggtcatata
    82381 gcctttcagc tctttcgcac ccgggccgct gaactcgatg cccagttgcg gcagacgcca
    82441 cagcagcgga gcaagatagc aatcgaccag gctgaactca tcgctcagga agtacggctt
    82501 ctgaccgaag accggcgcaa tcgccagcag ttcttcgcgc agttgcttac gtgcggcatc
    82561 tgcttcagaa gctgaaccgt tgatgatggt gttcatcagc gtgtaccagt ctttttcgat
    82621 gcgatgcatg tacagacggc tttcaccgcg agctaccggg taaacaggca tcagtggcgg
    82681 atgcgggaaa cgctcatcca gatattccat aatgatgcga gattcccaca gggtcagctc
    82741 acgatccacc agggtcggaa cgctctgatt cgggttgagg tcaatcagat cctgaggcgg
    82801 attgtccttt tccacgtgtt cgatctcgaa acttacacct ttctcagcca gcacaatgcg
    82861 gacctgatgg ctatagatgt cagtaggacc ggaaaacagc gtcattaccg aacgtttgtt
    82921 ggcagcgaca gccatgaaaa cctccaggta tagtcagaat ttttactgct accagccacc
    82981 aggtggccag tcagaagttg tgttacccag taaggaacga ctctctttgt tcgaaaatca
    83041 aacaaaaaat gagcaatacc cgacatttgg gcagaaaatt ggatgatagt ttaccagatt
    83101 ttgcgacctt tgtggtgagt cgatgccgga aatggggaaa aagagatgcg ctttagtctg
    83161 aaatagttga cttagtccct tattggcgat gtggtttttg ttttgcctgt ctgtcagacg
    83221 gtagcaaaaa gcaactttcc aatttttacg ctgattcaga atttaaccat aaaaaaaccc
    83281 gccgaagcgg gttttttcgc aaattgtttt ctgccggagc agaagccaat taacgtttgg
    83341 agaactgcgg acgacgacgt gctttacgca gaccgacttt cttacgttca acctgacgag
    83401 cgtcacgagt aacgaagcca gctttacgca gttcagaacg cagggactcg tcgtattcca
    83461 tcagagcgcg ggtgataccg tgacggatcg caccagcctg accagagata ccaccacctt
    83521 taacggtgat gtacaggtcc agtttctcaa ccatgtcgac cagttccagc ggctgacgaa
    83581 ctaccatgcg agcagtttca cgaccgaagt actgttccag agaacgttgg ttgattacga
    83641 ttttaccgtt gcccggtttg atgaaaacgc gagctgcgga acttttgcgg cgaccagtgc
    83701 cgtagtattg attttcagcc attgcctata atcccgatta gatgtcaaga acttgcggtt
    83761 gctgtgccgc gtggttgtgc tcgttacccg cgtaaacttt cagtttacgg aacatagcac
    83821 gacccagcgg gccttttggc aacatgcctt taaccgcgat ttcaatcaca cgctcaggac
    83881 ggcgagcaat catctcttca aaggtagctt gtttgatacc accgatgtgg ccggtgtggt
    83941 gatagtacac tttgtcagta cgcttgttgc cggttacagc aactttgtca gcgttcagaa
    84001 cgatgatgta atcaccggta tctacgtgcg gagtgtattc cgctttgtgc ttaccgcgca
    84061 ggcgacgagc cagttcagta gccagacggc ccagagtttt accggtcgcg tcaacaacat
    84121 accagtcgcg ttttacggtt tctggtttag ctgtaaaagt tttcattaaa agcttaccca
    84181 ataaatagtt acacgttggt gaacacccaa acgtcttcaa ttgttgaggt tcacacgaca
    84241 aagtccggca aacctacccc ttcgaatagc ctatgccagc acacaaaaag ttttgggaaa
    84301 aaaactttct tgtaacgtgg ggtcgcagga ttatagagaa gtcggggtca aagatcgacc
    84361 cctttttgtg atttgtgaca ggttttaacc cgccaaatgc tcgcgcttca gatactcttc
    84421 gctttgcatc tcttgcagac gtgacaggca acgctggaac tcaaacttca gccgatcgcc
    84481 ctgataaatt tcatacagcg gcacttctgc actcaccact aatttgacat ggcgctcgta
    84541 aaactcatcc accagcgcaa taaagcgccg cgcttcgctc tccatcaacc gcgtcataac
    84601 tggtacatca aacaacatga ccgtatgaaa gagacgtgag agcgcaatat agtcatgctg
    84661 actgcgggcg tcgacgcaca gcgtagtaaa agagaccgcc agcgtctggt tctcgacgcc
    84721 cattgtcgcc aatggccgat ggttgatttc taacgtcggt gaattttctc gtttcgcccc
    84781 cgccagcgcc aaccatagtt tatccatctg cgcccgggtt tcatcgttga gtggcgaaag
    84841 ccacagatgc gcctgagtga gtgtacgcag tcgataatca acaccagcgt ccacgttcat
    84901 tacatcacaa tgctgtttaa tggcatcgat tgcaggcaga aaacgcgcac gttgcaggcc
    84961 atttcgataa agttcatccg gcggaatatt tgacgtcgct accagggtaa tgccgcgggc
    85021 gaacagggct ttcatcagac cgccaagtag catggcgtcg gtaatatcag aaacaaaaaa
    85081 ttcgtcaaaa cagagcacgt cagtttcggc tttaaagcga tcggcaataa tttccagcgg
    85141 atcggtctgc ccctgtaagg cagttagctc ttcgtgcacc cgcagcataa aacggtgaaa
    85201 gtgcaggcgc tgtttccgct ctcccggcag gctttgatag aaaaggtcca tcagccaggt
    85261 tttcccgcgt cctacaccgc cccacatata taagccacgc actggcgtat gctttgtgtc
    85321 ttcgcgttta ccccacagct taccgacccg cgccattagc ccactcgtcc tgggggctgg
    85381 tggcctgcta ttgatgagtt cttgataaat aatttccagg cggctgacgg cctctttttg
    85441 aacgtcgtcg ggttgatggc tgccttcatt gagcgccttc aggtattgcg atgttggggt
    85501 aacgctttgc atgatcttat tgttattcct tgaataatcg gtgcgccgtt gttcacggtt
    85561 gacgaaaaaa aggccgttct acactacgcg atatgcagtc gggattccac ttctgtggaa
    85621 ttaacggtta tagtggcata atcagccgca ggcatggagc ctgaagccaa caccctacgg
    85681 aaacaaaaga caacgggaga tgttcatgac ctgggaatat gcgctaattg ggttagtcgt
    85741 cggcatcatt attggtgctg tggccatgcg ttttggtaat cgtaaactac gccagcaaca
    85801 ggcgttgcag tacgaactgg aaaagaataa agctgaactg gacgagtatc gcgaagagct
    85861 ggttagccac tttgcccgca gcgcggaatt actggatacc atggcgcacg actatcgcca
    85921 gctgtatcag cacatggcga aaagctccag cagcctgctg ccggaactgt ctgctgaagc
    85981 aaacccgttc cgtaatcgtc tggcagagtc tgaagccagc aacgatcagg caccggtgca
    86041 gatgcctcgc gactattctg aaggtgcatc cggcctgctg cgtactggcg cgaagcgcga
    86101 ctaatttatt tttcgggcgc agccattgcg ccctcctctt ctctccctcc ccgactatca
    86161 tttaatctgg tgtctcattg ttagccgtct gaaaattcaa taacatcaaa ctgttttgaa
    86221 tctcttttct tatcattcag gtacgagagc aggaataatg aaaaaacaaa cccagctgtt
    86281 gagtgcatta gcgttaagtg tcgggttaac tctctcggcg tcatttcagg ccgtcgcgtc
    86341 gattccaggc caggttgccg atcaggcccc tctccccagt ctggctccaa tgctggaaaa
    86401 agtgcttccg gcagtggtga gcgtacgggt ggaaggaacg gccagtcagg gacagaaaat
    86461 cccggaagaa ttcaaaaagt tttttggtga tgatttaccg gatcaacctg cacaaccctt
    86521 cgaaggttta ggttccggtg tcatcatcaa cgccagtaaa ggctatgtgc tgaccaacaa
    86581 ccatgtgatt aatcaggcac agaaaatcag tattcagctc aatgatgggc gcgagtttga
    86641 tgcaaaactg attggtagcg atgaccagag cgatatcgcc ctgttacaaa ttcaaaaccc
    86701 gagcaaatta acgcaaatcg ctattgccga ctccgataaa ttgcgcgtcg gtgattttgc
    86761 cgtagcggtc ggtaacccat ttggccttgg gcaaaccgcc acctctggca ttgtttccgc
    86821 attaggccgc agcgggttga atcttgaagg tctggaaaac tttatccaga cagatgcttc
    86881 cattaaccgc ggtaactccg gcggtgcact attaaacctt aacggtgagt taattggcat
    86941 caacactgca atccttgcgc ctggcggcgg gagcgtcggg attggatttg ccatccccag
    87001 taatatggcg cgaacactgg cgcagcagct tatcgacttt ggtgaaatca aacgcggttt
    87061 gttaggcatc aaaggcaccg agatgagtgc cgatatcgcc aaagccttca accttgacgt
    87121 gcagcgtggc gcgtttgtca gcgaagtgtt gccaggttct ggctcggcaa aagcgggcgt
    87181 caaagcgggc gatattatta ccagcctcaa cggcaaaccg ctgaatagct ttgctgagtt
    87241 gcgctctcgt atcgcgacca ccgagccggg cacgaaagtg aagcttggcc tgctgcgtaa
    87301 cggcaaacca ctggaagtag aagtgacgct cgataccagc acctcttcgt cggccagcgc
    87361 tgaaatgatc acgccagcgc tggaaggtgc aacgttgagc gatggtcagc taaaagatgg
    87421 cggcaaaggt attaaaatcg atgaagttgt caaaggaagc ccagctgctc aggctggctt
    87481 gcaaaaagac gatgtgatca ttggcgtcaa ccgcgatcgg gtgaactcga ttgctgaaat
    87541 gcgtaaagtg ctggcggcaa aaccggccat catcgccctg caaattgtac gcggcaatga
    87601 aagcatctat ctgctgatgc gttaatgtcg taaaccgggc atcaggctta cgtgtgatgt
    87661 ccggttaact cgtggtatgc tgctgccgtt ccctttttta atgacgcctc catcatgttt
    87721 gtgaagctct tacgttccgt tgcgattgga ttaattgtcg gcgctattct gctggttgcc
    87781 atgccttcgc tgcgcagcct taacccgctt tccactccgc aatttgacag taccgatgag
    87841 acgcctgcca gctataatct ggcggttcgc cgcgccgcgc cagcggtggt taacgtttac
    87901 aaccgtggtt tgaacaccaa ctctcacaac cagcttgaga tccgcacctt gggatccggt
    87961 gtaatcatgg atcaacgcgg ttatatcatc accaataaac acgtcatcaa cgacgccgat
    88021 cagatcatcg tcgccttaca ggatggtcgt gtatttgaag cattgctggt gggatctgac
    88081 tctctaaccg atctggcggt acttaaaatt aatgccactg gcggtttacc taccattcca
    88141 attaatgcac gtcgcgtacc gcacattggc gacgtagtac tggcgatcgg taacccgtac
    88201 aacctcgggc agaccattac ccaggggatt attagtgcca cgggtcgaat cggtctgaac
    88261 ccgaccgggc ggcaaaactt cctacaaacc gatgcttcca ttaaccacgg taactctggc
    88321 ggcgcgctgg tgaactcgct gggagaactg atgggcatta acacgctgtc gtttgataag
    88381 agtaacgatg gcgaaacgcc ggaaggtatc ggctttgcga ttcctttcca gttagcaacc
    88441 aaaattatgg ataagctgat ccgcgatggt cgcgtgatcc gcggctacat tggtatcggc
    88501 ggacgcgaga tcgcaccact gcacgcgcag agcggtggta tagatcaact gcaagggatc
    88561 gtggttaatg aagtgtcacc agacggccca gcggcgaatg cgggtattca ggtcaacgat
    88621 ctgattattt cggtggataa caaaccggcc atctctgctc tggagacgat ggatcaggtg
    88681 gcggaaattc gccctggttc ggtgatcccg gttgtagtga tgcgtgatga taagcagtta
    88741 acgctgcagg tcaccattca ggaatatccg gcaaccaatt aagtcgagtg ctcaaaacaa
    88801 aaaccggagt ctgtgctccg gttttttatt attcgctaat caattactta ttaacgaact
    88861 cttcgcccag ggcgatatct ttcttgagcg tatccagcat accttccagc gcgttctgtt
    88921 caaatgcgct cagggtaccg atagatttac gctcttccac gccgttttta cccagcagca
    88981 gcggttgaga gaagaaacgg gcgtactgac cgtcgccttc aacgtaggca cattcgacaa
    89041 cgccttgttc gccctgcagt gcacgaacca gagacagacc aaaacgtgca gctgcctggc
    89101 ccatagacag ggttgcagac ccgccaccgg ctttcgcttc aaccacctca gtacccgcgt
    89161 tctggatacg tttggtcaga tcagccactt cctgctcggt aaaactaacg ccaggaacct
    89221 gtgacagcag cggcagaatg gtaacaccag agtgaccgcc aataaccggc acttcaactt
    89281 cgcctggctg tttgcctttc agttccgcaa caaaggtgtt ggaacgaatg atatccagcg
    89341 tggtaacgcc gaacagtttg tttttgtcat aaacaccggc ttttttcagc acttcagcag
    89401 caatcgcaac tgtggtgtta accgggttag tgataatacc aatgcacgct ttcgggcagg
    89461 ttttcgcaac ttgctgtacc aggtttttca cgatgccggc gttaacgtta aacaggtcgg
    89521 aacgatccat acccggttta cgcgctacac ctgcagagat aagaacgaca tctgcgcctt
    89581 ccagcgccgg agtcgcatct tcaccagaaa aacctttgat tttcacagca gtagggatat
    89641 ggctcagatc gacagccaca ccgggagtca ctggagcgat atcatacaga gagagttctg
    89701 aacctgaagg cagttgggtt tttaacagta gtgcaagcgc ctggccaata ccgccagcag
    89761 cgccgaggac tgcgactttc atcctaaact ccttattata ttgataaact aagatatgtt
    89821 gctccgctgc cgcgacctta atccacaaaa ttgccgttta caatgaccac atctcaagaa
    89881 tgtgtagtca cgcaagttta gcgtttatgc atttaattgc cgtaatcagg aacctaactt
    89941 acgtaattaa cagtcgctta tgcattagcg caacattcca acaggtggtg acaatatacc
    90001 ctaccgttca gccaaaacaa catcaatttg ataacaatta atttactttt aagcagaatt
    90061 tgcatgccgt gacgcaggca tgtttctcaa taacgaaatt tgataaaatc ccgctctttc
    90121 ataacattat ttcagccttc ttcagggcta actgtttgca taaaaattca tctgtatgca
    90181 caataatgtt gtatcaacca ccatatcggg tgacttatgc gaagctcggc taagcaagaa
    90241 gaactagtta aagcatttaa agcattactt aaagaagaga aatttagctc ccagggcgaa
    90301 atcgtcgccg cgttgcagga gcaaggcttt gacaatatta atcagtctaa agtctcgcgg
    90361 atgttgacca agtttggtgc tgtacgtaca cgcaatgcca aaatggaaat ggtttactgc
    90421 ctgccagctg aactgggtgt accaactacc tccagtccat tgaagaatct ggtgctggat
    90481 atcgactaca acgatgcagt tgtcgtgatt cataccagcc ctggcgcggc gcagttaatt
    90541 gctcgcctgc tggactcact gggcaaagca gaaggtattc tgggcaccat cgctggcgat
    90601 gacaccatct ttaccacccc tgctaacggt ttcacagtca aagacctgta cgaagcgatt
    90661 ttagagctgt tcgaccagga gctttaatct ctgccccgtc gtttctgacg gcggggaaaa
    90721 tgttgcttat ccctctcaat cccctgcttt cccctgcgat tcatttaacg aatagtgcgt
    90781 tttaccgcga catatcattc acgcaatgaa tacatgcggt aaaaaaagca cattatgcaa
    90841 aattcattat
//
LOCUS       DAZFWP020000002        65576 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFWP020000002 DAZFWP020000000
VERSION     DAZFWP020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.002"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            90..653
                     /gene="srlA"
                     /locus_tag="ACHK0Z_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /gene_synonym="gutA"
     CDS             90..653
                     /gene="srlA"
                     /locus_tag="ACHK0Z_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGU4500178.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            650..1609
                     /gene="srlE"
                     /locus_tag="ACHK0Z_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /gene_synonym="gutE"
     CDS             650..1609
                     /gene="srlE"
                     /locus_tag="ACHK0Z_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGU4500179.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1620..1991
                     /gene="srlB"
                     /locus_tag="ACHK0Z_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /gene_synonym="gutB"
     CDS             1620..1991
                     /gene="srlB"
                     /locus_tag="ACHK0Z_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU4500180.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1995..2774
                     /gene="srlD"
                     /locus_tag="ACHK0Z_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             1995..2774
                     /gene="srlD"
                     /locus_tag="ACHK0Z_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU4500181.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2879..3238
                     /gene="gutM"
                     /locus_tag="ACHK0Z_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             2879..3238
                     /gene="gutM"
                     /locus_tag="ACHK0Z_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU4500182.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3305..4078
                     /gene="srlR"
                     /locus_tag="ACHK0Z_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /gene_synonym="gutR"
     CDS             3305..4078
                     /gene="srlR"
                     /locus_tag="ACHK0Z_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU4500183.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4071..5036
                     /gene="gutQ"
                     /locus_tag="ACHK0Z_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             4071..5036
                     /gene="gutQ"
                     /locus_tag="ACHK0Z_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU4500184.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALAASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5033..6547)
                     /gene="norR"
                     /locus_tag="ACHK0Z_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     CDS             complement(5033..6547)
                     /gene="norR"
                     /locus_tag="ACHK0Z_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU4500185.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLIYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6734..8173
                     /gene="norV"
                     /locus_tag="ACHK0Z_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     CDS             6734..8173
                     /gene="norV"
                     /locus_tag="ACHK0Z_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU4500186.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            8170..9303
                     /gene="norW"
                     /locus_tag="ACHK0Z_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             8170..9303
                     /gene="norW"
                     /locus_tag="ACHK0Z_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU4500187.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9529..11781)
                     /gene="hypF"
                     /locus_tag="ACHK0Z_RS00510"
                     /old_locus_tag="pgaptmp_000102"
     CDS             complement(9529..11781)
                     /gene="hypF"
                     /locus_tag="ACHK0Z_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU4500188.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11934..12461)
                     /gene="hydN"
                     /locus_tag="ACHK0Z_RS00515"
                     /old_locus_tag="pgaptmp_000103"
     CDS             complement(11934..12461)
                     /gene="hydN"
                     /locus_tag="ACHK0Z_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU4500189.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12610..13620)
                     /gene="ascG"
                     /locus_tag="ACHK0Z_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             complement(12610..13620)
                     /gene="ascG"
                     /locus_tag="ACHK0Z_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU4500190.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13880..15337
                     /gene="ascF"
                     /locus_tag="ACHK0Z_RS00525"
                     /old_locus_tag="pgaptmp_000105"
     CDS             13880..15337
                     /gene="ascF"
                     /locus_tag="ACHK0Z_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU4500191.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            15346..16770
                     /gene="ascB"
                     /locus_tag="ACHK0Z_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             15346..16770
                     /gene="ascB"
                     /locus_tag="ACHK0Z_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU4500192.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     KITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16884..17303)
                     /locus_tag="ACHK0Z_RS00535"
                     /old_locus_tag="pgaptmp_000107"
     CDS             complement(16884..17303)
                     /locus_tag="ACHK0Z_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4500193.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(17374..18128)
                     /locus_tag="ACHK0Z_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /pseudo
     CDS             complement(17374..18128)
                     /locus_tag="ACHK0Z_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18171..18641)
                     /gene="hycI"
                     /locus_tag="ACHK0Z_RS00545"
                     /old_locus_tag="pgaptmp_000109"
     CDS             complement(18171..18641)
                     /gene="hycI"
                     /locus_tag="ACHK0Z_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU4500194.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(18634..19044)
                     /gene="hycH"
                     /locus_tag="ACHK0Z_RS00550"
                     /old_locus_tag="pgaptmp_000110"
     CDS             complement(18634..19044)
                     /gene="hycH"
                     /locus_tag="ACHK0Z_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU4500195.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(19041..19808)
                     /gene="hycG"
                     /locus_tag="ACHK0Z_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             complement(19041..19808)
                     /gene="hycG"
                     /locus_tag="ACHK0Z_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU4500196.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(19808..20350)
                     /gene="hycF"
                     /locus_tag="ACHK0Z_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             complement(19808..20350)
                     /gene="hycF"
                     /locus_tag="ACHK0Z_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU4500197.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(20360..22069)
                     /gene="hycE"
                     /locus_tag="ACHK0Z_RS00565"
                     /old_locus_tag="pgaptmp_000113"
     CDS             complement(20360..22069)
                     /gene="hycE"
                     /locus_tag="ACHK0Z_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU4500198.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22087..23010)
                     /gene="hycD"
                     /locus_tag="ACHK0Z_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             complement(22087..23010)
                     /gene="hycD"
                     /locus_tag="ACHK0Z_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU4500199.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23013..24839)
                     /gene="hycC"
                     /locus_tag="ACHK0Z_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     CDS             complement(23013..24839)
                     /gene="hycC"
                     /locus_tag="ACHK0Z_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU4500200.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(24836..25447)
                     /gene="hycB"
                     /locus_tag="ACHK0Z_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             complement(24836..25447)
                     /gene="hycB"
                     /locus_tag="ACHK0Z_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU4500201.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(25572..26033)
                     /gene="hycA"
                     /locus_tag="ACHK0Z_RS00585"
                     /old_locus_tag="pgaptmp_000117"
     CDS             complement(25572..26033)
                     /gene="hycA"
                     /locus_tag="ACHK0Z_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU4500202.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26245..26595
                     /gene="hypA"
                     /locus_tag="ACHK0Z_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             26245..26595
                     /gene="hypA"
                     /locus_tag="ACHK0Z_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4500203.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26599..27471
                     /gene="hypB"
                     /locus_tag="ACHK0Z_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             26599..27471
                     /gene="hypB"
                     /locus_tag="ACHK0Z_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU4500204.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            27462..27734
                     /gene="hypC"
                     /locus_tag="ACHK0Z_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             27462..27734
                     /gene="hypC"
                     /locus_tag="ACHK0Z_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU4500205.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            27734..28855
                     /gene="hypD"
                     /locus_tag="ACHK0Z_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             27734..28855
                     /gene="hypD"
                     /locus_tag="ACHK0Z_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU4500206.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            28852..29862
                     /gene="hypE"
                     /locus_tag="ACHK0Z_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             28852..29862
                     /gene="hypE"
                     /locus_tag="ACHK0Z_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU4500207.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            29936..32014
                     /gene="flhA"
                     /locus_tag="ACHK0Z_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             29936..32014
                     /gene="flhA"
                     /locus_tag="ACHK0Z_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU4500208.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32051..32404)
                     /gene="ygbA"
                     /locus_tag="ACHK0Z_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             complement(32051..32404)
                     /gene="ygbA"
                     /locus_tag="ACHK0Z_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU4500209.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(32436..32615)
                     /locus_tag="ACHK0Z_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             complement(32436..32615)
                     /locus_tag="ACHK0Z_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4500210.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            32691..35252
                     /gene="mutS"
                     /locus_tag="ACHK0Z_RS00630"
                     /old_locus_tag="pgaptmp_000126"
     CDS             32691..35252
                     /gene="mutS"
                     /locus_tag="ACHK0Z_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU4500211.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35358..36014
                     /gene="pphB"
                     /locus_tag="ACHK0Z_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             35358..36014
                     /gene="pphB"
                     /locus_tag="ACHK0Z_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4500212.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(36065..36832)
                     /gene="ygbI"
                     /locus_tag="ACHK0Z_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             complement(36065..36832)
                     /gene="ygbI"
                     /locus_tag="ACHK0Z_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGU4500213.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            37028..37936
                     /gene="ltnD"
                     /locus_tag="ACHK0Z_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             37028..37936
                     /gene="ltnD"
                     /locus_tag="ACHK0Z_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HGU4500214.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            37933..39195
                     /gene="otnK"
                     /locus_tag="ACHK0Z_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             37933..39195
                     /gene="otnK"
                     /locus_tag="ACHK0Z_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HGU4500215.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWTQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            39192..39830
                     /gene="ygbL"
                     /locus_tag="ACHK0Z_RS00655"
                     /old_locus_tag="pgaptmp_000131"
     CDS             39192..39830
                     /gene="ygbL"
                     /locus_tag="ACHK0Z_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4500216.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            39835..40611
                     /gene="ygbM"
                     /locus_tag="ACHK0Z_RS00660"
                     /old_locus_tag="pgaptmp_000132"
     CDS             39835..40611
                     /gene="ygbM"
                     /locus_tag="ACHK0Z_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU4500217.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            40700..42064
                     /gene="ygbN"
                     /locus_tag="ACHK0Z_RS00665"
                     /old_locus_tag="pgaptmp_000133"
     CDS             40700..42064
                     /gene="ygbN"
                     /locus_tag="ACHK0Z_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HGU4500218.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(42103..42357)
                     /locus_tag="ACHK0Z_RS00670"
                     /old_locus_tag="pgaptmp_000134"
     CDS             complement(42103..42357)
                     /locus_tag="ACHK0Z_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU4500219.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRDT
                     EPLRRTSREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(42368..43795)
                     /locus_tag="ACHK0Z_RS00675"
                     /old_locus_tag="pgaptmp_000135"
     CDS             complement(42368..43795)
                     /locus_tag="ACHK0Z_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU4500220.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(43795..44388)
                     /locus_tag="ACHK0Z_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             complement(43795..44388)
                     /locus_tag="ACHK0Z_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU4500221.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            44535..44942
                     /locus_tag="ACHK0Z_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             44535..44942
                     /locus_tag="ACHK0Z_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4500222.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(45063..46055)
                     /gene="rpoS"
                     /locus_tag="ACHK0Z_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             complement(45063..46055)
                     /gene="rpoS"
                     /locus_tag="ACHK0Z_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGU4500223.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46118..47212)
                     /gene="nlpD"
                     /locus_tag="ACHK0Z_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             complement(46118..47212)
                     /gene="nlpD"
                     /locus_tag="ACHK0Z_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU4500224.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(47397..48023)
                     /gene="pcm"
                     /locus_tag="ACHK0Z_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             complement(47397..48023)
                     /gene="pcm"
                     /locus_tag="ACHK0Z_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU4500225.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(48017..48778)
                     /gene="surE"
                     /locus_tag="ACHK0Z_RS00705"
                     /old_locus_tag="pgaptmp_000141"
     CDS             complement(48017..48778)
                     /gene="surE"
                     /locus_tag="ACHK0Z_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU4500226.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(48759..49808)
                     /gene="truD"
                     /locus_tag="ACHK0Z_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             complement(48759..49808)
                     /gene="truD"
                     /locus_tag="ACHK0Z_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU4500227.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(49805..50284)
                     /gene="ispF"
                     /locus_tag="ACHK0Z_RS00715"
                     /old_locus_tag="pgaptmp_000143"
     CDS             complement(49805..50284)
                     /gene="ispF"
                     /locus_tag="ACHK0Z_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU4500228.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50284..50994)
                     /gene="ispD"
                     /locus_tag="ACHK0Z_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     CDS             complement(50284..50994)
                     /gene="ispD"
                     /locus_tag="ACHK0Z_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU4500229.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(51013..51324)
                     /gene="ftsB"
                     /locus_tag="ACHK0Z_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             complement(51013..51324)
                     /gene="ftsB"
                     /locus_tag="ACHK0Z_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU4500230.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(51518..51841)
                     /gene="ygbE"
                     /locus_tag="ACHK0Z_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             complement(51518..51841)
                     /gene="ygbE"
                     /locus_tag="ACHK0Z_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU4500231.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(51891..52496)
                     /gene="cysC"
                     /locus_tag="ACHK0Z_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             complement(51891..52496)
                     /gene="cysC"
                     /locus_tag="ACHK0Z_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU4500232.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGVLEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(52496..53923)
                     /gene="cysN"
                     /locus_tag="ACHK0Z_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             complement(52496..53923)
                     /gene="cysN"
                     /locus_tag="ACHK0Z_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU4500233.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(53925..54833)
                     /gene="cysD"
                     /locus_tag="ACHK0Z_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             complement(53925..54833)
                     /gene="cysD"
                     /locus_tag="ACHK0Z_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU4500234.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55085..56122
                     /gene="iap"
                     /locus_tag="ACHK0Z_RS00750"
                     /old_locus_tag="pgaptmp_000150"
     CDS             55085..56122
                     /gene="iap"
                     /locus_tag="ACHK0Z_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU4500235.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   56143..56904
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56265..56293
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(57001..57294)
                     /gene="cas2e"
                     /locus_tag="ACHK0Z_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             complement(57001..57294)
                     /gene="cas2e"
                     /locus_tag="ACHK0Z_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU4500236.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(57294..58214)
                     /gene="cas1e"
                     /locus_tag="ACHK0Z_RS00760"
                     /old_locus_tag="pgaptmp_000152"
     CDS             complement(57294..58214)
                     /gene="cas1e"
                     /locus_tag="ACHK0Z_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU4500237.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(58211..58861)
                     /gene="cas6e"
                     /locus_tag="ACHK0Z_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             complement(58211..58861)
                     /gene="cas6e"
                     /locus_tag="ACHK0Z_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU4500238.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(58843..59589)
                     /gene="cas5e"
                     /locus_tag="ACHK0Z_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             complement(58843..59589)
                     /gene="cas5e"
                     /locus_tag="ACHK0Z_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU4500239.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(59600..60673)
                     /gene="cas7e"
                     /locus_tag="ACHK0Z_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             complement(59600..60673)
                     /gene="cas7e"
                     /locus_tag="ACHK0Z_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU4500240.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPGFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            complement(60688..61221)
                     /gene="casB"
                     /locus_tag="ACHK0Z_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /gene_synonym="cse2"
     CDS             complement(60688..61221)
                     /gene="casB"
                     /locus_tag="ACHK0Z_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU4500241.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            complement(61218..62780)
                     /gene="casA"
                     /locus_tag="ACHK0Z_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /gene_synonym="cse1"
     CDS             complement(61218..62780)
                     /gene="casA"
                     /locus_tag="ACHK0Z_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU4500242.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(62878..>65576)
                     /gene="cas3"
                     /locus_tag="ACHK0Z_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             complement(62878..>65576)
                     /gene="cas3"
                     /locus_tag="ACHK0Z_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HGU4500243.1"
                     /translation="RKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDV
                     AAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPE
                     LKIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTG
                     HHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSWY
                     ISATVVLADWTGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGIN
                     TLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQG
                     LFFGLPTMATANAMYDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLIG
                     SEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKI
                     LLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQQ
                     EAPLLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQG
                     KCIAWIRNSVDDAIQVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYC
                     SLQRASQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRDG
                     KDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMP
                     HAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKYVLNFRPGYAANINDYL
                     PEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLE
                     GDAFRQWCVEQRQDPEMANVILVTDDESCGYSAREGLIGKVG"
BASE COUNT        15469 a        17877 c        16943 g        15287 t
ORIGIN      
        1 gcgatcaaaa taacactttt aaatctttca atctgattag attaggttgc cgtttggtaa
       61 taaaacaata aatcctgaag gagagaacaa tgatagaaac cattactcat ggtgcagagt
      121 ggtttatcgg gctgttccaa aagggcggag aggtgtttac cgggatggtg accggcattc
      181 ttccgctgtt gattagcctg ctggttatca tgaacgcact gattaatttt atcggtcagc
      241 atcgtattga acgttttgct caacgttgcg ccggtaaccc tgtttcccgt tacctactgt
      301 taccgtgcat tggcacgttt gtcttttgca atccgatgac cctaagcctg ggtcgcttta
      361 tgccggaaaa gtacaaaccc agctactacg cggcggcctc ttatagctgc cactcaatga
      421 atggcctctt cccccatatc aaccctggcg aactgtttgt ttatcttggc attgccagcg
      481 gtctgacaac gctgaacctg ccacttggcc cactggcggt gagttatctg ctggttggtc
      541 tggtcaccaa tttcttccgc ggctgggtga ccgatctgac caccgccatt tttgagaaaa
      601 agatgggcat tcaacttgaa caaaaagttc acctggcagg agcaacatca tgacgcatat
      661 tcggatcgaa aaaggaacgg gtggctgggg cggcccgctt gagctgaaag ccacgccggg
      721 aaaaaaaatc gtctatatca ccgccggtac ccggcctgcg attgttgaca aactggcaca
      781 gcttactggc tggcaggcta ttgacggatt taaagaaggt gaacccgcgg aggcggaaat
      841 tggtgtcgcg gtaatcgact gtggcggcac attacgctgc ggcatctatc cgaaacgacg
      901 tattcccacc attaatatcc actcgacggg caagtccggt ccgctggcgc agtacattgt
      961 ggaagatatt tatgtctctg gcgtaaaaga agaaaacatc actgtagtgg gtgatgcgac
     1021 accacaaccc tcttccgtgg gccgtgacta tgacaccagt aagaaaatca ccgaacaaag
     1081 cgatggttta ctggcgaagg tgggaatggg catggggtcc gccgttgcgg tgctgtttca
     1141 atctggtcgt gacaccatcg acactgtatt aaaaaccatt ctgccgttta tggcattcgt
     1201 ctcggcgctc attggcatca ttatggcttc tggccttggt gactggattg cccacggtct
     1261 tgctccgctg gcgagccatc cactgggtct ggtcatgctg gcgctcatct gctccttccc
     1321 actgctttca cctttcctcg gcccaggcgc agttatcgca caggttatcg gcgtattgat
     1381 tggcgtgcag attggtctcg gcaatattcc gccgcatctg gctttaccgg cactgtttgc
     1441 catcaacgcg caggcggcct gcgacttcat cccggtcggt ttgtcgctgg cggaagctcg
     1501 tcaggacacg gttcgcgtcg gtgtcccttc tgtactggtg agccgctttt taaccggcgc
     1561 gccgactgta ctgatcgcct ggtttgtctc cggttttatc tatcaataga ggctgaaaca
     1621 tgaccgttat ttatcagacc accatcaccc gtatcggcgc gagtgccact gacgccctca
     1681 gcgaccagat gctcatcacc tttcgtgaag gcgcgccagc ggacctcgaa gagtattgct
     1741 tcattcattg ccacggcgag ttgaaaggtg aactccatcc tggtttgcaa tttacgctcg
     1801 ggcagcatcg ctatccggtg accgccgttg gcagcgtggc ggaagacaac cttcgcgaac
     1861 tgggtcatgt caccctgcgc ttcgatggtt taaacgaagc ggaatttccg ggcactgtcc
     1921 atgtggcagg ccctgtaccc gacgatatcg cgccgggatc ggttttgaag tttgaatctg
     1981 ttaaggagta aaaaatgaat caggttgccg ttgtcatcgg tggtgggcaa accttaggcg
     2041 cgttcctgtg ccacggtctg gctgccgagg ggtatcgcgt cgcggttgtc gatattcaga
     2101 gcgacaaagc cgcaaatgtg gcacaagaaa ttaacgccga atatggtgaa ggtatggcgt
     2161 acggttttgg tgctgacgcc accagcgagc aaagcgttct ggcgctctct cgtggggtag
     2221 atgaaatctt tggtcgcgtg gatttgctgg tctatagcgc cggaatagca aaagcagcct
     2281 ttatcagcga cttccagctc ggcgattttg accgttcgct acaagtgaat ctggtgggtt
     2341 atttcctgtg tgcccgtgaa ttttcgcgtt tgatgatccg cgacgggatt cagggtcgca
     2401 ttattcagat caactcgaaa tccggcaaag tgggtagcaa acacaactct ggctacagcg
     2461 cagcgaaatt tggtggcgtc gggctgactc aatcactggc gctggatctg gcggagtacg
     2521 gcattacggt gcattcactg atgctcggta acctgctgaa atcgccgatg ttccagtcac
     2581 tgttgccaca atacgcgacc aagctgggta tcaaaccgga tcaagtcgag cagtattaca
     2641 tcgacaaagt accgctcaaa cgcggctgcg attatcaaga tgtgctgaat atgctgctgt
     2701 tctacgccag tcctaaggcg tcgtactgca ccggacagtc gatcaatgtc accggcggtc
     2761 aggtgatgtt ctgatcaaca gcggagatcc gttaaggatc tccgtgagac tatagaatgc
     2821 ctgatgcgct acgctcatca ggcatacagg acttccgcca ctacattaag gaaaagttat
     2881 ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt gcgcaactgg ccttaggcgg
     2941 ctggcaaatt tctcgtttta accgtgcctt cgacacacta tgccagcaag ggcgggtcgg
     3001 cgtgggccgt tccagcgggc gctttaaacc gcgggtcgtg gtcgccatcg ccctggacga
     3061 tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg accgtcttcg cccgaccgca
     3121 aaaaattccc gcaattaccg gtatgcatgc gggtgattta cagcccgatg tgatctttcc
     3181 ccatgatcca ctatcacaga atgctctatc attggcgctt aaactgaaac gtggataatt
     3241 tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga aacctaaatc aggtaatcac
     3301 gcccatgaaa cctcgtcagc gtcaggccgc cattctggag tatctgcaaa agcagggtaa
     3361 atgctcggtt gaagaattgg cgcaatactt tgacaccaca ggcacaacca ttcgcaaaga
     3421 tctggtcatt ctggaacatg ccggaaccgt cattcgtact tatggcggag tggtgttgaa
     3481 taaagaggaa tccgatccgc ctatcgatca taaaacgctc atcaacaccc acaagaaaga
     3541 gctgattgca gaagctgccg ttagttttat tcatgatggc gactcaataa ttcttgatgc
     3601 tggcagtacc gttttgcaga tggtccccct gctctcgcgc tttaataaca tcacggtgat
     3661 gaccaacagt ctgcatatcg tcaatgcgct atctgaactg gataacgaac aaaccatcct
     3721 gatgccaggc ggaacatttc gcaaaaaatc ggcctcattt cacgggcaac tggcagagaa
     3781 tgccttcgag catttcacct tcgacaaatt attcatgggt actgacggca tcgatctcaa
     3841 tgcgggcgta accaccttca acgaggtcta taccgtcagt aaggcaatgt gcaatgccgc
     3901 gcgcgaagtg attttaatgg cggactcatc aaagtttggc cgtaaaagcc ccaacgtagt
     3961 ttgcagtctt gaaagcgtcg ataagctgat taccgacgca ggtatcgatc cggcgtttcg
     4021 tcaggcgctg gaagagaaag ggatcgatgt gatcataacc ggagagagca atgagtgaag
     4081 cactactgaa cgcgggacgt cagacgttaa tgctggagtt gcaggaagca agccgtttac
     4141 cggaacgtct gggcgatgat tttgttcgcg ccgccaatat catcctgcac tgcgaaggca
     4201 aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg taagaaaatc gccgcaacac
     4261 ttgccagtac cggcacaccg gctttttttg tccatccggc agaagcactg cacggcgatc
     4321 tggggatgat tgaaagccgc gatgtgatgc tgtttatctc ttactccggt ggcgcgaagg
     4381 aactggatct gattattccg cgtctggaag ataaatctat cgcgctgctg gcgatgaccg
     4441 gcaaaccgac gtcaccgctg ggcctggcgg caaaagcggt gctggatatc tccgtagaac
     4501 gcgaagcctg cccgatgcac cttgcgccga cctccagcac cgtcaatacc ctgatgatgg
     4561 gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggttt taatgaagaa gattttgccc
     4621 gctcccaccc agctggggca ctgggcgctc gcttgctgaa taaagtgcat catctgatgc
     4681 gccgtgatga cgctatcccg caggtagcgt tagccgccag cgtgatggat gcgatgctgg
     4741 agctcagccg caccggtctg gggctggtgg cggtatgtga cgctcaacaa caggtacaag
     4801 gcgtctttac cgacggcgat ttacgtcgct ggctggttgg cggcggcgca ctcaccacgc
     4861 cagttaatga agcgatgacg acaggcggca ccacattaca ggcgcaaagc cgtgccatcg
     4921 acgccaaaga gatcctgatg aagcgcaaaa tcactgccgc accggtggta gatgaaaacg
     4981 gcaaactcac cggcgcaatt aacttgcagg atttctatca ggccgggatt atttaatcct
     5041 tcaatcccag acgtttcgcc agccgatgca ggttggcgac gtcagtttcc agcattcgcg
     5101 cgcaggcagc ccagttgtga tgattttgtg ccagtgcctg gcgaatggtt tcacgctgga
     5161 acgcttctgt cgcttcacgc agattttgct taacaacggg caccgctgcc gcttctggcg
     5221 gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc aagaatcact tcatcgccgc
     5281 tgcgagtggc tctcgacagt actaccgccc gatgaatagc atgttccagt tcgcgcacgt
     5341 tccccggaaa actgtagtgt tgcagtaaat ttcgcgcccc ggcacttaat accacgcggg
     5401 agagccccag ccgcaaacga cactgctcgc agaaataccc cgccagcaga atgacatcat
     5461 cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac gctcaggcga tgaaacaagt
     5521 cagcgcgaaa tcgccctgcc agtacctctt cgcgtaagtc gcggttagtc gccgccagca
     5581 cgcgcacatc gacccgcaaa ctgcggtcat cgccaacgcg ctgaatatcg ccatactgca
     5641 acactcgcag caacttggcc tgcaaagcca acgacaactc gccgatctca tcgagaaaca
     5701 gtgtgccgtt atccgccatt tcaaacttcc cgctgcggtt actgatagcg ccagtaaacg
     5761 cccctttcac atgcccgaac agttcacttt ccgccacact ttccggcagt gcggcacagt
     5821 tgagatagat cagcggattc accgcccgtg gcgaggcttc atgaatcgct ttcgccacca
     5881 gctccttacc ggttcccgtc tcaccgctga ttaagacgtt gagatcggac gccgccacaa
     5941 tctcaatctc ttttttcaat tgcgtcatgc ctggcgataa gccaatcatc tgcgtctgtt
     6001 tcaccgcttc aaacggcgcg gcatcgcccg gcagcatatt ctggctttcc agttgctcaa
     6061 tcagcaacgc attgcttaac gctcccgccg ccagcgcagc aatcagccgt aactcttcat
     6121 cgctgaaaac atcgaactga tcgggctgca tcccgtcgag cgtcaatgcg ccaatcaggt
     6181 tttgcccggc aaacaatggc agaccaacgc aggcgtgaac cttcagactc tcctgcccag
     6241 gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc aggaaagcgc accacgtccc
     6301 cggcgcgggc aatcgcttca agccgtggat gcccttccag cgcaaagcgt ctaccgagta
     6361 catcctttgc cagaccgtcg atggcaagcg gaataaactg ccgcgaatcg taacgtagca
     6421 acgcagacgc atcgcactcc agcacctgac gtagcgtggt gatcaggcgc tgaaaacgat
     6481 cctgatgacc aatcccacgc tgcaattcga tggcgatatt cgccagcaca tcaacggaaa
     6541 aactcatctt tgcctcactg tcaatttgac tatagatatt gtcatatcga ccatttgatt
     6601 gatagtcatt ttgactactc attaatgagc atacttttat ttatatagta aaaacaatca
     6661 gataaaaaac tggcacacaa tctgcaatta gcaagacatc tttttagaac acgctgaata
     6721 aattgaggtt gctatgtcta ttgtggtgaa aaataacatt cattgggttg gtcaacgtga
     6781 ctgggaagtg cgtgattttc acggcacgga atataaaacg ctgcgcggca gcagttacaa
     6841 cagctacctc atccgcgaag aaaaaaacgt gctgatcgac accgtcgacc ataaattcag
     6901 ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg gcggatatcg attacatcgt
     6961 gattaaccat gcagaagagg accacgctgg ggcgctgacc gaactgatgg cgcaaattcc
     7021 cgatacgccg atctactgta ctgccaacgc tatcgactcg ataaatggtc atcaccatca
     7081 tccggagtgg aattttaatg tggtgaaaac tggcgacacg ctggatatcg gcaacggcaa
     7141 acagctcatt tttgtcgaaa caccaatgct gcactggccg gacagcatga tgacttacct
     7201 gacaggcgac gcggtgctgt tcagtaacga tgctttcggt cagcactact gcgacgagca
     7261 tctgttcaac gatgaagtgg atcagacgga gcttttcgag cagtgccagc gttactacgc
     7321 caatatcctg acgccgttca gccgcctggt aacgccgaaa attaccgaga tcctgggctt
     7381 taacttacca gtcgatatga tagcgacctc ccacggcgtg gtctggcgcg ataacccgac
     7441 gcaaattgtc gagctgtacc tgaagtgggc cgctgattat caggaagaca gaatcaccat
     7501 tttctacgac accatgtcga ataacacccg catgatggct gacgctatcg cccagggaat
     7561 tgcggaaact gacccacgcg tggcggtgaa aattttcaac gtcgcccgaa gcgataaaaa
     7621 cgaaatcctg accaatgtct tccgctcaaa aggcgtgctg gtcggcactt cgacgatgaa
     7681 taacgtgatg atgccgaaaa tcgccgggct ggtggaggag atgactggtt tacgcttccg
     7741 taacaaacgc gccagtgctt tcggctctca cggctggagc ggcggtgcgg tggatcgtct
     7801 ttccacgcgc ctgcaggatg cgggtttcga aatgtcgctt agcctgaaag ccaaatggcg
     7861 accagaccag gacgctctgg agttatgtcg tgaacacggt cgcgaaatcg cccgtcagtg
     7921 ggcgctcgcg ccgctgccgc agagcacggt gaatacggtg gttaaagaag aaacctctgc
     7981 caccacgacg gctgacctcg gcccacggat gcagtgcagc gtctgccagt ggatttacga
     8041 tccggcaaaa ggcgagccaa tgcaggacgt tgcgccagga acgccgtgga gtgaagttcc
     8101 ggataacttc ctctgcccgg aatgctccct cggcaaagac gtctttgaag aactggcatc
     8161 ggaggcaaaa tgagtaacgg cattgtgatc attggttcgg gcttcgccgc ccgccaactg
     8221 gtgaaaaata ttcgcaaaca ggacgccact attccattaa ccctgattgc cgccgacagc
     8281 atggatgagt acaacaaacc tgacctcagc catgttatca gtcaggggca acgtgccgac
     8341 gaccttaccc gccagacggc gggtgaattt gccgagcagt ttaatctgcg cctgtttccg
     8401 cacacctggg tgacggatat cgatgccgaa gcccgtgtgg tgaaaagcca gaataatcag
     8461 tggcaatacg acaagctagt gctggcaacc ggtgccagcg cctttgtccc gccagtgcct
     8521 gggcgtgagt taatgctgac gttaaatagt cagcaagagt atcgcgcctg tgaaacgcaa
     8581 ctgcgggatg cccgacgcgt gttgattgtc ggtggtggct tgattggtag cgaactggcg
     8641 atggattttt gtcgtgcagg caaagcggtc acgctaatcg acaacgctgc cagtattctg
     8701 gcgtcattaa tgccaccgga agtaagcagc cgcttgcagc atcggttgac ggagatgggc
     8761 gttcatctgt tgttgaaatc tcagttgcag gggctggaaa aaacagattc tggcattctg
     8821 gcaacgctgg accgccagcg cagtatcgaa gtggatgcgg taattgccgc caccggactg
     8881 cgcccggaaa ccgccctggc acgacgcgcc gggctgacga ttaatcgcgg cgtttgcgtc
     8941 gatagttatc tgcaaaccag taatgccgat atttacgcgc tgggcgattg cgcggaaatt
     9001 aacggtcagg tattgccgtt cctccagccg attcaactta gcgcgatggt gctggcaaaa
     9061 aatcttctcg gcaataacac gccactgaaa ctaccggcaa tgctggtgaa aatcaaaacg
     9121 ccggaattac cgctgcatct ggcaggcgaa acccagcgtc aggatttacg ctggcagatt
     9181 aataccgaac gccagggaat ggtggcgcgc ggcgttgacg atgctgacca gcttcgcgcc
     9241 tttgtggtca gtgaggatcg gatgaaagag gcatttggat tgttgaaaac gttgccgatg
     9301 taggtgggct actgtgccta aaatgtcgga tgcgacgctg gcgcgtctta tccaacctac
     9361 ggggagcgca tgtgttggtc acatactgcg ttcatgtcgc atccacctac tgtgcccaaa
     9421 atgtcggatg cgacgctggc gcgtcttatc cgacctacgg ggacgcatgt gtaggccgga
     9481 taaggcgttt acgccgcatc cggcaatggt gtccaaatgc aacacgtttt atccgttctg
     9541 gacttcaccc gctaaccaac gcgccgcagc aataaccccc tgccccagag acaaaccgcc
     9601 atcacccgcc ggtaaactct gtggaaagag caatgtgaaa tcagcgagat aatgcgccag
     9661 acgtgcacgc agcaaacggt tatgaataac cccgccgcta aataccagcg tagtgatacc
     9721 acgcatcgtg gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa
     9781 cgcccacgcg cgttgattaa ccggtgcctg ccagttcagc cactgctgcc agaaagtggc
     9841 gagatccagt tgattgtcca ccagcggcat tgtcaccgga tgcgtcactc cgtggcacga
     9901 agctgcgagt gcctccagag cacaagccgc ttcaccttca taacttaacg tggctggcgc
     9961 acagcccagt gccgccgcca cggcatcgaa caaccgccca cacgatgacg ccagcggcgc
    10021 gttaattcca cgctcaatgg cccgcgccag cacgctccag ttttgctgtt gcacacttgc
    10081 tgtttcagaa taattctgcc actccggcac aaagcgcagg cactgcgcca gcaggtttcg
    10141 ccacggctgc gtcgccgcca aatcgccacc cggaagcgcc actgcaggca agccgcccag
    10201 gtgctcacat tcgcgatagt tcacccgcag acactcgccg ccccacaaag cgccgttctc
    10261 ccccataccg ataccatcga gcgtcaaagc aatgacatca ccgccatcca gcggccactg
    10321 atgctctgcc agacacgccg ctgcgtgggc atgatgatgc agcaccgttt gcgtcggcat
    10381 attcatttcg ctcgcccact ggctggagac atagcccgga tgcacgtcat gcacaacgta
    10441 ttgcggagtg aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc actgcatctg
    10501 gatgccatcg tcacttaaat cgcccagatg ctgactcaac accgcttgtt caccgcgcac
    10561 caggcagaag gtgtttttca gatccgcgcc aagacacagc acaggcggaa catttttaaa
    10621 gcccggaggc aaaaccagcg catccggcac atacccgcgt gaacggcgca gcatttcgcc
    10681 gctttcgcgc accaccgaat catccatccg ctgcacgatg tcgcggttat gtatcaagaa
    10741 tccgtcggca atgccctgca aatccgccag cgcctgttcg ttgctgatag ctggtggttt
    10801 accgctcagg ttgccggagg tcatcaccag cgggcattgc agttcctgta acagcaaatg
    10861 ctggagcggg ttcgcaggca acattacccc gacttcgtta aggtcagggg cgatatcatc
    10921 acaaagctca ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg gcgtggtaag
    10981 caactggcgc gcagcgtctg gtaaaccgtc agccactggc aacatgaccg ccagcggttt
    11041 cgccgggcga tgtttgcgcg cccgcagtgt cgccaccgcg ttactgttac gtgcatcgca
    11101 ggcaagatga aatccgccaa tccctttgat ggcgacaatg ttgcccattt ttaactgtgc
    11161 gatagccgcc tgtaatgccg cctcttgttc tgcatgttca ccatgactta cccattcaag
    11221 atgtgggcca cactccgggc aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc
    11281 gcggtactct ttgtcacaag ccggacacaa cggaaacgcc gccatcacgg taaacgggcg
    11341 gtcgtaaggc atggcgcgaa taatggtgaa acgcgggccg cagtgggtac agttgataaa
    11401 cggataacga taacgccgtt cgcctggggt attcatttcg gcaagacagg cagggcaagt
    11461 agcggcatcg ggaacaattt gcgtattcat ggtgccgcct gtgctctggc gtatagtgaa
    11521 ctcggtgggc agttgtgacc agatgtacgg ctcacgctcg acgctatcaa tacgcgccag
    11581 cggcgggcag tgctgatgca attgaacaag aaacgtttcc gggtcttcca gcagccggac
    11641 ttctacgcca tcgccgtcat tacagacatc gccgtgaaga tttaattgct gtgccagctg
    11701 ccagacaaac ggacgaaaac cgacgccctg cactttgcct cgaatacgca gttggacacc
    11761 gcaagatgtg ttttttgcca ttgagttatt cccgccatca tgaattgcgt aacccgccct
    11821 gccggacacg actgcgtcgt atccggcagt ctcaggtcgg cgataccgcc agctccgtat
    11881 tctacgaata tttccgggaa ttcctttgat gccagaacag ttctataaga tttttagaac
    11941 atcagcgccg tacggcgacg tttttctgcg ctcagttgtt caagtttatt acgatcgaca
    12001 caaatcagcg catgggtcgg gcaagccgcc atacacgccg ggccgtcttc gcgatggttg
    12061 cacaggtcgc atttattggc ttcagctttg tcagcccgta cattcagccc cgcgccgctg
    12121 ttgcggatca ccggacgtac caccacttcc atcgcgccat acgggcaagc caccacgcag
    12181 gttttgcaac caatgcaacg ttcctgcatc acatgaacaa accctttatc acggctgata
    12241 gcaccattcg ggcagacgtt agcgcacggt gcatcttcac actgacggca gactgtcgcc
    12301 gtggaaatgt tcacaccttt aatgacatgg attcgcggta aaaaggtttc cggggtcagc
    12361 gatgcacagt cctgattttc ctgatgagaa accacgcacg ccacttcaca ggtacggcaa
    12421 ccaatacatt tactcgcgtc agcaatgatg aaacggttca tcaaattctc cagcaatgac
    12481 agttaatgcg ccgatacatt cacaaatcat gccagttttt aatttgctgt tatttaagga
    12541 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    12601 tgacaatgtt tatcgcgaaa gggcaatgag tgagtcgcgg cggatcagtt ttccgctgaa
    12661 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    12721 ctgaatcatc tctgttaccg gaatttttac gctggagagc gccggaacgg tgtagggggc
    12781 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    12841 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    12901 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    12961 accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    13021 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    13081 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    13141 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    13201 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    13261 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    13321 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    13381 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca gccccagcgt
    13441 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    13501 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    13561 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    13621 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    13681 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat
    13741 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    13801 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    13861 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc
    13921 ggcactgggc ggcgttgata acatctcggc agtcacgcac tgtatgacgc gtttgcgctt
    13981 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    14041 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    14101 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    14161 acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcagcaccat
    14221 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    14281 cgagatgagc ggcgtgttga caaaaggatc gccgacctta accattctga atgtgattgg
    14341 tgacggtgct ttcttcttcc tgccgctaat ggtcgcggct tcggccgcca tcaaatttaa
    14401 aaccaatatg tcgctggcga ttgccatcgc aggtgtgctg gtacatccga gctttattga
    14461 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    14521 ggtgaaatat acctacacgg tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    14581 cgaacgctgg gtggatcgca ttacaccggc tgtgaccaaa aacttcctca agccaatgct
    14641 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    14701 cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt
    14761 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    14821 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    14881 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    14941 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    15001 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    15061 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    15121 agcgccgggg ctatttacca gcgtgcagtt cttcgatccg gcgaatccaa tgagcatcgt
    15181 ctgggtattc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    15241 cggctttgag gatattcctg ttgaggaagc gactgccgag gcgcgaaagc atcagagcgt
    15301 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    15361 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    15421 ggtggcaaag ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    15481 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    15541 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    15601 aaggttttcc gtacctcaat tgcctggagc cgcctctttc cgcagggcga taaaatcacg
    15661 cccaatcagc agggcattgc tttttaccgt tctgtctttg aagagtgtaa aaagtacggt
    15721 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    15781 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgccag aacctgcttt
    15841 gaagcatttg atggtctggt gaaatactgg ctaaccttca atgaaatcaa cattatgttg
    15901 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    15961 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat
    16021 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac
    16081 agctgcaaac cggaagatgt ctgggcggcg ctggagaaag atcgggaaaa cctgtttttt
    16141 atcgatgtgc aggcgcgcgg cgcgtatccg gcttactctg cccgcgtatt tcgcgaaaaa
    16201 ggggtaacca tcaacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt
    16261 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaacagc
    16321 agtgcggcga acgtggtgaa atcgctgcgt aatccgtatc tacaggtgag cgactggggc
    16381 tggggaattg atccactcgg tctgcgtatc accatgaata tgatgtacga tcgttatcag
    16441 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    16501 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    16561 gcgattgcag atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtt
    16621 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    16681 gcaggcaacg gcacgctgac gcgcacgcgt aagaaatcgt tctggtggta taaaaaagta
    16741 attgccagta atggggaaga tttagagtaa gtaaccgtgc cggatgcggc gtgaacgcct
    16801 tatccggcct acatgtcgca cgaaccatga aggcccgata agcgcagcgc atcgggcaat
    16861 ctggggtttg gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct
    16921 gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc
    16981 agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc
    17041 aacgtgcgaa gtaacgccac gccaaccggc attgccgcaa cggctttgtc gaactctgca
    17101 aacttttcgt tgttgttttc atactctgca atgcggctcg ccagaaagtc gataagtgga
    17161 ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct
    17221 tctgagtcac tgcctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca
    17281 gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgac gtctgtctgc
    17341 acgtaatccc cctcatagcc actctgcaaa aagtcagttc tggttcaccc cataaagcac
    17401 atcccccttt atcaagcaac gctgcgtctg cgccagatct tccgtttgcg ggtcgcgccc
    17461 ggcgatgggt ttgaggacga gtttacggtt gggggtataa gtaaaacgcg tgagcgcggg
    17521 atagcttaac ttgccaaacc agccgtgaac acgctgccat ttggcgtctt ttaccgatac
    17581 agactggtta tccagtaacc ggaatcaaac tgcatatcca gcaattgcgt gtggccattc
    17641 ctttgtaaat ccagcaaatc atcaatgcca gtttcacttg cggtataaaa accatcggac
    17701 tgaaaaacac acgggcgacc ttgctgatta aaggtcatca gaatcaaaaa cgcgttaggt
    17761 gccagaccat ttcctgaaat accccgccag ataaccagat cctgaatgcc gttacgatca
    17821 agatcggcac ggtaaagttg cgcattaccc gccatatttt gcagcactcc cacaggcaca
    17881 gaccaggatt taccggcgcg atctttaccg ttcaattgcc attcactatc atcatttggc
    17941 atcttttcaa gcgtcacgcc agcaacgccg acttttctaa aatgccagtt ttgctcggca
    18001 tcataaggca tggtcaggag aggtttcagc gcccgctttt gcgcgtattc gctgtcactc
    18061 attggcagat attcggtaaa gctggtggcc tgtgcaggca gcactaagct caataaaaaa
    18121 gcgcacatcc ctgttctgat gttcaattcc ctgaattccc tgatggagaa ttattcttct
    18181 tccaccgcta actgcgcaaa tccaccattt ccttcccagc cttccagtcg ttgataaacg
    18241 gtttctaccg catctttaat cggctgggtc atcgggtagt aaaagccgac gatatccggc
    18301 tgaatgccga ggaaaatcac ttcaccaatg tcttctttta actggtcgat aaggtaattc
    18361 aacggcatgt tatgggtagt catcataaac atctcggcga tatcatccgg gtcgatgatg
    18421 cggatttcgc cggggtttag ccccatatcc gtggcgtcga caatcagcag tcgtgtcggg
    18481 cgcagttcac ggatggcgac gatgtcgttt tccggtgcac taccgccgtc aatcaccacc
    18541 cagttacctt tcggcgcggc ggcgcacttt tccgccagca gcgggccagc gccatcatcg
    18601 cccatcatgc tattgccaac acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa
    18661 atagatggcg ttttcctgat gaatatcatg cagcatactt aacagcattg tactccattc
    18721 cttctgctgc gaagtttgcg ctgcccgggc tttatcgaat gcgttggcca gcatcggcac
    18781 atggttaatg tcgatgacga tctcgccata tttcggtacg ccttccattt tgcggcgggc
    18841 ttcgctgccc gcctccagag tggcaatcca tgccagatat tcatcccatg ggcaggtgag
    18901 cgccgcttcc aggcaatcga taaccccaag gtggtgacca atcgccaggc tgtaatagac
    18961 cacctgctgc gcctcggcgg gcgtggcatc gttctcatca ataaatttac ggctcagttg
    19021 actgaacacc accttttcac tcatcggata cgcgcctctt caacaacatg attcagatgg
    19081 ctgacaatct cgttcagacg cggatcgttt tccgcttcca gccagcgtgc aacctgttct
    19141 tcgccctgcc ctaactgtgt caggaagtca tcggcaatct gacgaccgta acgataacct
    19201 gccaggcggc gtgcttcgcg atccactttc acacgcagcg gctgcaccat atcgccatgc
    19261 aggatttccg ccggttgttc atcctgctca cccgggccac gggcgtgaat tttctgctcc
    19321 agcaggccga gcgccattgc aaagccgtac agcgtggcgg caggcgttgg cgggcaacca
    19381 gggatgtaga catctaccgg gacgatttta tccgtaccgc cccacacgca gtagagatcg
    19441 tggaagatcc cgccactgtt accgcaggca ccgtaggaga tacagatttt cgggtccggc
    19501 gcggactgcc acgcacgcag cgcaggggat cgcattgcac gggtgaccgc gccggtaaac
    19561 agtaaaatat ccgcatgacg cggtgaagga acgactttaa tgccgaagcg ttctgcatca
    19621 aacagcggcg aaagcgtggc gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc
    19681 acgcggtaaa cgtaggcaga acgtttgatt tttttcagta acgacgcctt catgctggcg
    19741 atggattcat ccaccgtcat cgggaccgga atgccgttgg cgtcacgggg gcctaataaa
    19801 ttgctcatca gatggcctct ttcatatggc gagtcagttc aatacggtcg gacggcacca
    19861 ggcatttctg gcgcttacat tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc
    19921 ggctgtcgcc gttgtgctta agtagcgcaa tggcgtagtc gatctctttc tggacagcga
    19981 aaggacgatt gcagacgcga cagttgcaca gcgcgaagcg ggactgttgc aggaagtctt
    20041 ctttcttcca caccgccagt tcgtactctt gcgacagttt gatcgccgcc gtcgggcaaa
    20101 cttcttcgca gcgtccacag aagatgcagc gtccaagatt gaactcccac gcaagctctc
    20161 ctgtggcgag gtcggtttca accgttaagg cgtttgacgg gcaggcattg acgcaggccg
    20221 cgcagccgat gcactgctgc gggttctgct ctggcttacc acggaagttt ttatcaaccg
    20281 caatcggctc cagcggataa gacgaggtcg ccgtgccggt tttgataact tttttgataa
    20341 aggtaaacat ggcgattcct tatttcagcg gcgagttttt acgctcaatg ctgtagcgct
    20401 cgagttcttt gtacggcacc actttgctct tcttcttacg tacatcgacc accgtcatgc
    20461 ggtcggtaca ggagtagcaa gggtccaggc taccgataat cagcggcgca tcggaaacgg
    20521 tattgccacg cagcatgtag cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc
    20581 gccagcggta cagcttctgg ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg
    20641 gcgcttcggc aaagcccagc gcgaaacggt gcggaatgta ggtaaagcct tccaccatca
    20701 gcggaccgcc cggcaggtta tccagaccgt agtcgatcat gttcagcgcg gtataaactt
    20761 cgttgatacg cactttcaga cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca
    20821 ttggcagcag gccataaccg acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc
    20881 tggcgcgaac catcgggcca acgttactga aatcgcgggc gatttccggg tccagacgac
    20941 caatgccgac ggtgcgctgt tccatgttcg gcgtactcag cagcacatcc accagctcct
    21001 gcacttcacg acgcatctgt tgcgccagct ggcgggtctg gatcatgtca tctttcaaca
    21061 gatcgcgacg aatcccgccg atcaggttca ggccgtaggt tttacgcgca ccggtgagga
    21121 tctctgccat tttcatggag gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa
    21181 agccggtaaa gtggcaggcg agaccgaggt tgagcagatg cgagtgcagg cgttccacct
    21241 ccagcagaat ggcgcggatc atctgcgcac gttctggcac ctgaataccc atcgcgtttt
    21301 ctaccgacgt ggtgtaggca gtgctgtggg caaagccgca gatcccgcac acacggtcag
    21361 agaggaaggt cacttcgttg taacccatac gggtttccgc cagtttttcc atgccgcgat
    21421 gaacgtagaa cagacggtag tcggcgtcga taatgttttc gccatcgacg aacagacgga
    21481 agtggcccgg ttcgtcagaa gtgacgtgca gcggaccaat cggcacgacg ttgtttttct
    21541 tgtcgcccag ttcgttgatg aactcgtagg tttcagcatc ggtagtcggt gccggacgct
    21601 gacgataatc catgctgtct ttacgcagcg gataaagttc atccggccag tcatccggca
    21661 gcaccagacg acgttcatcc ggcagaccaa ccggaatcaa accgtacata tcgcgcactt
    21721 cgcgctcgcc ccacaccgcc gccggaacgc gcggcgtcac ggacggatac tccggtttgt
    21781 tggcgtcaac ttcgacgcga accgttaccc aacacttggt gcccttctcc atcgacagca
    21841 cgtaataaac ggcgtaatga ccattcagtt tgcgttcgtc gttaccaaac agcaccgaca
    21901 gccagccacc ctgcttgtag tagagaaact ccaccacttc cggcaggtag ttcaccttca
    21961 cggtcacggt cagctgatct ttggtctgcc aggcgtggtc cagcacgacg cccggaaatg
    22021 cctcattcag cgcggcgaga taatgttgac ctaatttttc ttcagacatg ctcaaactct
    22081 ctttaatcac gccgccagca aggagacgaa cgctaaaaat gcaaagccaa acccagccca
    22141 ggtaatgcgc ggagtaatat caagacgcag acgggccatg ctgttttcga acagcgcgat
    22201 aaccaggacg ccgacgacca gtttgacgat ggcaatcacc agcgccagca gcagcccacc
    22261 ggcggtgaag gtttccatct gtccccacgg gataaatacg ccgacgaaca tctgcagcac
    22321 caccagctgt ttcaggctga taccccattt catgacgcca aagccgctgc cgctgtattc
    22381 agagagcggg ccttcctgca gctcctgctc ggcttccgcc aggtcgaacg gcagtttgcc
    22441 catttcgata aaggtggcga acgcacaggc acaaagcgcc agtaccagcg ggatgctctg
    22501 gctcagcggc cagtgataaa cggtgtcggt gatgttgctg atgttggtgg aaccggcaac
    22561 ctgtgcggca acccacagac caagcagcag catcggttcg accagcacgc caagcatcgc
    22621 ttcacggctc gcgccgatag cggtaaacgg gctaccggta tccagaccag aaatggcaaa
    22681 gaagaaacgc gcgatggcaa agagatacag taaggtgatt aaatcaccca gttgcggcag
    22741 cggagaaccg acggtcacca ccggcagcgc agtagcgata gtcagcatga cgcccaccat
    22801 cacatacggc gtcaggcgga acacccagcc ggaggcatcc gggccaacgc tctggcggcc
    22861 cagcagtttg ataatgtcac gatactcctg caacacaccc ggcccacgac ggttatgtaa
    22921 gcgggctcgc gccacgcggg taataccgga gagcagcggc gcaacggcaa atagcaccag
    22981 cgcctgaatt aacggatata aaacactcat tctcaggctc ctcgtgaaac aataatcacc
    23041 accagcaccg ccagttcaac cagcgccatc cggcggaaca gcaacgcact cccctcgcac
    23101 tgccagcccg gcaccagaga caccggattc agccatttgc gtagtttcag caccggcgca
    23161 aacgcctgtt tcaccggcat ggcaaaaccg tgagcggtaa tcaccattga tttttcgtgg
    23221 tcgtagccgc acacccaggc cgcaccgcgg gaacgcgacg gcaaacgatc gcctttgcaa
    23281 atcgccataa tgatgaatgg cagcagcggg caggcaatca gcagcaacgt gatcatcggt
    23341 tgagaaacgg tggtgttagc aggctccagc ggcagaggta cagcagcaga gagcatcggc
    23401 agcagccacg gcgcagcaac accgccaatt acgcagcaaa tcgccagtgc cactacgctt
    23461 acgctcatca ggagcggcgc acaggtggcg ttttcggctt ctttggtacg cggcgcgccg
    23521 aggaacgtga cgccatagac tttcgccata cacatcaccg ccagcgcacc ggtaattgcc
    23581 agacccacag cgagcagcgg ccccagcaga cgggcaacaa acgcgccact attgctcagt
    23641 ttgaaaaagg attgatagat aacccattcc ccggcaaacc cgttcagcgg cggcagcgca
    23701 gccattgcca tcaacccgac taacatggcg atggagataa ccggcatttt cttgccaata
    23761 ccaccgagtt tttcgatatc gcgatgaccg gtacggaacc agacgctccc cgccccgagg
    23821 aacagtacgc ttttgaacag gctatggtta agcagatggt acagaccacc gaccagacca
    23881 agggcaatca gcgccggttg ttcgagcgcg atacccgtta cgccagcgcc cagccccagc
    23941 aggataatgc cgatattttc cagggtgtgg taagccagca ggcgctggat gttgtgttcc
    24001 atcaacgcat acagaccacc gacgaacgcg gtgatcatgc cgagcaccag cagcgcgatc
    24061 ccccaccaca gcggcgcgtt accacccagc agcgacaggg ttaaaatgcc cagcaggcca
    24121 attttcatga ctaccgtaga aaacaacgcg gcagctggcg cagaggcgtt agcatgtgcc
    24181 tgcggcaccc agccgtgcag cggaataatc ccggccagca ggccaaagcc aatcacaccg
    24241 agcagccaga tatcggaacc gagcggcagc tgttgcatac gcatatccag caggcgcaga
    24301 tccagcgtgc cataacgctg ccacagcagc cagcaagcaa tcgccagcag cagagtgcca
    24361 agacgcccca gcgcaaacca cagtttgccc tctttgctgt tgctggtgag gaacaccgca
    24421 cacaaggcca tgacttcggc cattaccacg aacatgccga ggttgctggc aatgacggcg
    24481 cagacggcgg cagccatcaa catattgatc tgcaagccgt tgcacttcac ctgcgcgtgg
    24541 cgatgccagt caatgttata gaggctgaca aacagaccgc aaaggccgag ggtaatcagc
    24601 caaatcgcgt taagcgggga gatttgcaca tcgtagctta ccagcgacag cgcaccgctc
    24661 acgccaaccg cgccagtcag cacagtgaag cccgcggctg ccgtatacag gctaccaacc
    24721 gcgccgccaa ttccggctat ccagccactt agcgtctttt gaaaagaaaa gagaaatgcc
    24781 agaacagcgg cggcaataaa ccacgccacg ccgctattga tcagggaaat tgcgctcatt
    24841 tagcctctcc actttgagcc tgctgaaaca aggtgagatc gccaaagtcc gtattaaaag
    24901 tcagctcacg cttacgtttg ctgacgcggg cgatatcggt gttatccacc agatgcaggg
    24961 ctttggtcgg gcacatccgc acgcaggccg gaccttgttc atcaaaactg caaaggtcac
    25021 atttgacggc gatcgcgcga atacccggca cccagtcaag cagcgtactg atacgcgccg
    25081 gagcaggcgg tgccggtggc gctttcgggg tattggcatt tgcaggaata tccagcggac
    25141 ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat gccgcacagc ttgcagctta
    25201 cgcacaggct ttcattcaac tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc
    25261 agaccaccgc gcagggtgca tcttcacagt ggtgacagag ctgcggcgca gattcttttt
    25321 catttagcat cactctcagg cgcggcattg attgcaggcc gtgctggcga tgcgtctctg
    25381 aacaggcggc ctcacaagtg tggcagccga tacagagcgt ggagtcagca attacaaaac
    25441 gattcaccag gcattcctca ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt
    25501 cattttcgac actcatcgac acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc
    25561 aaagcctcag ctcatgctgc cgggctttgt ccctttacca gttggcttaa attcaccggc
    25621 acattgtttt caatggcggt gtataagctg tcgtaaacgc cagcgatttt ttcgagatag
    25681 tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc catcaatcat gccgtcaatg
    25741 ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccat cttttgtttc gacgtaatac
    25801 tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa
    25861 aggacgaaac agagttcttt gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc
    25921 gataacgtga tcccctgaac cagcgaattg cagtagatgt gccactggtc ctgtaggcga
    25981 cgatgccgct gtgcgatgta atcggctttc tcgcttattt cccaaatagt cattgtcagg
    26041 ttacccgttt aacagagatg ccagctttaa gcattttttg tgccaacttt taatttattg
    26101 ttattaaaga gatttttaag ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg
    26161 acatcatcga cattattcac cgcagggata atcaacactg gcacaattat tgcttgtagc
    26221 tggcaatagt taatgggagg cgatatgcac gaaataaccc tctgccaacg ggcactggaa
    26281 ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg taactggggt ctggctcaaa
    26341 attggcgcat tttcttgtgt cgaaaccagc tctcttgcct tttgttttga tctggtttgc
    26401 cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg
    26461 tgtgaaacat gccaacagta tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag
    26521 tgtcatggtg acatgctaca gattgtggca gacgacggtt tacagattcg gcggatagaa
    26581 atagaccagg agtgagcgat gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc
    26641 gagggtgatg aacataaccc tcattccgcg tttcgtagcg cgccatttgc cccggcggca
    26701 cgcccgaaga tgaaaatcac cggcattaaa gcgcctgaat ttacccccag ccagactgaa
    26761 gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc acgcaccggg catgagccag
    26821 cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc
    26881 aaccgcgcgc gctttgctgc ccgcaagcaa ctggtgctca acctggtttc cagccctggt
    26941 tccggtaaaa ccaccctgct gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc
    27001 gcggttattg aaggtgacca gcaaaccgtg aacgatgccg cacgcattcg cgctaccggc
    27061 acaccagcga ttcaggtgaa caccggtaaa ggctgccatc ttgacgcaca gatgattgcc
    27121 gacgccgcac cgcgtctgcc actggacgat aacggtattc tgtttatcga aaacgttggc
    27181 aacctcgtat gcccggccag cttcgatctc ggtgaaaaac acaaagtggc ggtgctttcc
    27241 gttaccgaag gtgaagacaa accgctgaaa tatccgcata tgtttgccgc cgcctcgctg
    27301 atgctgctca acaaagttga cctgttgccg tatctcaact ttgacgttga aaagtgcatc
    27361 gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc ttatttccgc caccagcggc
    27421 gaagggatgg accagtggct gaactggctg gagacacagc gatgtgcata ggcgttcccg
    27481 gccagatccg caccattgac ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg
    27541 atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt
    27601 gggtactggt acacgttggc tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca
    27661 ctctcgacgc cttacaaaac atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg
    27721 gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt
    27781 aattgagcat ctgcgcgaac gtgcttcaca tctctcttac accgccgaac gccctctgcg
    27841 gattatggaa gtgtgtggcg gtcataccca cgctatcttt aaattcggcc tcgaccagtt
    27901 actgccggaa aacgttgagt ttatccacgg tccggggtgc ccggtgtgcg tactgccgat
    27961 gggtagaatc gacacctgcg tggagattgc cagccatccg gaagtcatct tctgtacctt
    28021 tggcgacgcc atgcgcgtac cagggaaaca gggttcgctg ttgcaggcaa aagcacgcgg
    28081 tgccgatgtg cgcatcgttt actcgccgat ggatgcgttg aaactggcgc aggagaatcc
    28141 aacccgcaaa gtggtgttct tcggcttagg ttttgaaacc accatgccga ccaccgccat
    28201 cactctgcaa caggcgaaag cgcgtgatgt gcagaatttt tacttcttct gccagcatat
    28261 tacgcttatc ccgacgcttc gcagtttgct ggaacagccg gataacggta tcgacgcgtt
    28321 cctcgcgccg ggccacgtta gtatggttat cggcactgat gcctataatt ttatcgccag
    28381 cgattttcat cgtccgctgg tggtggctgg tttcgaaccg cttgatctac tgcaaggcgt
    28441 ggtcatgctg gtggagcaga aaatagcggc ccacagcaag gtagagaatc agtatcgtcg
    28501 ggtggtaccg gatgccggta acctgctggc gcaacaggcg attgccgatg tgttctgtgt
    28561 caacggcgac agcgaatggc gcggcttagg cgtgattgaa tcttctggtg tacacctgac
    28621 gccagattat caacgattcg atgccgaagc gcatttccgc ccggcaccgc agcaggtctg
    28681 cgatgacccg cgcgcacgtt gtggtgaagt attaacgggc aaatgtaagc cgcatcaatg
    28741 cccgctgttt ggtaacacct gtaatcctca aaccgcattt ggtgcactga tggtctcctc
    28801 cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag gagagtgaag cgtgaataat
    28861 atccaactcg cccacggcag cggcggccag gcgatgcagc aattaatcaa cagcctgttt
    28921 atggaagcct ttgccaaccc gtggctggca gaacaggaag atcaggcacg tcttgagctg
    28981 gcgcagctgg tagcggaagg cgaccgtctg gcgttctcca ccgacagtta cgtcattgac
    29041 ccgctgttct ttcctggcgg taatatcggc aagctggcga tttgcggcac tgcgaatgac
    29101 gttgcggtca gtggcgctat tccgcgctat ctctcctgtg gctttatcct cgaagaagga
    29161 ttgccgatgg agacactgaa agccgtagtg accagcatgg cagaaaccgc ccgcacggca
    29221 ggcattgcca tcgttactgg cgatactaaa gtggtgcagc gcggcgcggc agataaactg
    29281 tttatcaaca ccgctggcat gggcgcaatt ccggcgaata ttcactgggg cgcacagacg
    29341 ctaaccgcag gcgatgtatt actggttagc ggcacgctcg gcgaccacgg ggcgactatc
    29401 cttaacctgc gtgagcagct ggggctggat ggcgaactgg tcagcgactg cgcggtactg
    29461 acgccgctta ttcagacgct gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc
    29521 cgtggtggtg tgaacgcggt agttcatgag ttcgcggcag cctgcggttg tggtattgaa
    29581 atttcagaag cggcactgcc ggttaaacct gccgtgcgcg gcgtttgcga actgctggga
    29641 ctggacgccc ttaactttgc caacgaaggc aaactggtga tcgccgttga acgcaacgcg
    29701 gcagagcaag tgctggcagc gttacattcc catccactgg gaaaagacgc ggcgctgatt
    29761 ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc
    29821 ctcgatttac cacacgccga accgcttccg cgtatatgct aataaaattc taaatctcct
    29881 atagttagtc aatgaccttt tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc
    29941 atatacaccg atgagtgatc tcggacagca agggttgttc gacatcactc ggacactatt
    30001 gcagcagccc gatctggcct cgctgtgtga ggctctttcg caactggtaa agcgttctgc
    30061 gctcgccgac aacgcggcta ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta
    30121 cgcatcgcgt gaaaaagaca cccccattaa atatgaagac gaaactgttc tggcacacgg
    30181 tccggtacgc cgcattttgt cgcgccctga tacgctgcat tgcagttacg aagaattttg
    30241 tgaaacctgg ccgcagctgg tcgcaggtgg gctatacccc aaatttggtc actattgcct
    30301 gatgccactg gcggcggaag ggcatatttt tggtggctgt gaatttattc gttatgacga
    30361 tcgtccctgg agcgaaaaag agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt
    30421 cgtcaccgaa caaatccaga gccgcgtcgt caacaatgtc gactatgagt tgttatgccg
    30481 ggagcgcgat aacttccgca tcctggttgc cattaccaac gcggtgcttt cccgcctgga
    30541 tatggacgaa ctggtcagcg aagtcgccaa agaaatccat tactatttcg acattgacga
    30601 tatcagcatt gtcttacgca gccaccgtaa aaacaaactc aacatctact ccactcacta
    30661 tcttgataaa caacatcccg cccacgaaca gagcgaagtc gatgaagccg gaaccctcac
    30721 cgaacgcgtg ttcaaaagta aagagatgct gctgatcaat ctccacgagc gggacgattt
    30781 agccccctat gaacgcatgt tgttcgacac ctggggcaac cagattcaaa ccttgtgcct
    30841 gttaccgctg atgtctggcg acaccatgct gggcgtgctg aaactggcgc aatgcgaaga
    30901 gaaagtgttt accactacca atctgaattt gctgcgccag attgccgaac gtgtggcaat
    30961 cgctgtcgat aacgccctcg cctatcagga aatccatcgt ctgaaagaac ggctggttga
    31021 tgaaaacctc gccctgaccg agcagctcaa caatgttgat agtgaatttg gcgagattat
    31081 tggccgcagc gaagccatgt acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga
    31141 cagtaccgtg ctgatcctcg gtgaaactgg cacgggtaaa gagctgattg cccgcgcgat
    31201 ccataatctc agtgggcgta ataatcgccg catggtcaaa atgaactgcg cggcgatgcc
    31261 tgccggattg ctggaaagcg atctgtttgg tcatgagcgt ggggctttta ccggtgccag
    31321 cgcccagcgc attggtcgtt ttgaactggc ggataaaagc tctctgttcc tcgacgaagt
    31381 gggcgatatg ccactggagt tacagccgaa gttgctgcgt gtattgcagg aacaggagtt
    31441 tgaacgtctc ggcagcaaca aaatcattca gacggacgta cgtttaattg ccgcgactaa
    31501 ccgcgatctg aaaaaaatgg tcgccgaccg tgagttccgt agcgatctct attaccgcct
    31561 gaacgtattc ccgattcacc tgccgccact acgcgagcgt ccggaagata ttccgctgct
    31621 ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg cgcaatatcg acagcattcc
    31681 tgccgagacg ctgcgcacct tgagcaacat ggagtggccg ggtaacgtac gcgaactgga
    31741 aaacgtcatt gagcgcgcag tattgctaac acgcggcaac gtgctgcagc tgtcattgcc
    31801 agatattgct ttaccggagc ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg
    31861 cgaagatgaa tatcagttga ttgtgcgcgt gctgaaagaa accaacggcg tggttgccgg
    31921 gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg accctgctgt cacggatgaa
    31981 gcggcttgga attgataaat cggcattgat ttaactgcaa attgccggac agatctgcct
    32041 gtccggcata ctattcacga gtttttttcg gacgatattt ttccggcagt tctggcaccg
    32101 gacgcttgtc atcgatgaga tgacgcacgg ttaagatcgg atgacgccac agcattcgcg
    32161 gcccggccca acgcataatc tgtttcatct cttcacgctt tgctggctgg taacagtgca
    32221 ccggacactg cttacaggct ggtttctctt cgccgaacac acatttatcc agccgctttt
    32281 gcgcgtaaac aaacaacgcc tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg
    32341 cttgataaag atcgatcatt tttttaatcg tcagtttttc acgagagata cgcttgccgg
    32401 acatgctgcc tccacctcat taagatgtat ttatattaca tcttaatctt aaagggcact
    32461 atgactctaa agaagaaagg ttcgccaacc gacgcaattt tgcgtacttg cttcataagc
    32521 atcacgcaaa agctgcaaaa cagcatcttt cccggaacca gcatcaagaa ctcaccgttc
    32581 gcttcttccc ctgaaatgat taactccggt atcatgtgcg ccttatgtga ttacaacgaa
    32641 aataaaaacc atcacacccc atttaatatc agggaaccgg acataacccc atgagtgcaa
    32701 tagaaaattt cgacgcccat acgcccatga tgcagcagta tctcaggctg aaagcccagc
    32761 atcccgagat cctgctgttt taccggatgg gtgattttta tgaactgttt tatgacgacg
    32821 caaaacgcgc gtcgcaactg ctggatattt cactgaccaa acgcggtgct tcggcgggag
    32881 agccgatccc gatggcgggg attccctacc atgcggtgga aaactatctc gccaaactgg
    32941 tgaatcaggg agagtccgtt gccatctgcg aacaaattgg cgatccggcg accagcaaag
    33001 gtccggttga gcgcaaagtt gtgcgtatcg ttacgccagg caccatcagc gatgaagccc
    33061 tgttacagga gcgtcaggac aacctgctgg cggctatctg gcaggacagc aaaggtttcg
    33121 gctacgcgac gctggatatc agctccgggc gttttcgcct gagcgaaccg gccgaccgcg
    33181 aaacgatggc ggcagagctg caacgcacta atccggcgga actgctgtat gcagaagatt
    33241 tcgccgagat gtcgctgatt gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt
    33301 ttgaaatcga caccgcgcgc cagcagttga atctgcaatt tggcactcgc gatctggtcg
    33361 gttttggcgt cgagaacgcg ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg
    33421 cgaaagatac ccaacgtacg actctgccgc atattcgttc catcaccatg gaacgtgagc
    33481 aggacagcat cattatggat gccgcgacgc gtcgtaatct ggaaatcacc cagaacctgg
    33541 cgggtggtgc ggaaaatacg ctggcttctg tgctcgactg caccgtcacg ccgatgggca
    33601 gccgtatgct gaaacgctgg ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc
    33661 gccagcaaac tatcggcgca ttgcaggatt tcaccgccga gttgcagccg gtactgcgtc
    33721 aggtcggcga cctggaacgt attctggcac gtctggcttt acgaactgct cgcccacgcg
    33781 atctggcccg tatgcgccac gctttccagc aactgccgga gctgcgtgca cagttagaaa
    33841 ctgtcgatag tgcaccggta caggcgctac gtgagaagat gggcgagttt gccgagctgc
    33901 gcgatctgct ggagcgagca atcatcgaca caccgccagt gctggtacgc gacggtggtg
    33961 ttatcgcatc gggctataac gaagagctgg atgagtggcg cgcgctggct gacggcgcga
    34021 ccgattatct ggagcgtctg gaagtccgcg agcgtgaacg taccggcctg gacacgctaa
    34081 aagttggctt taatgcggtg cacggctact acattcaaat cagccgtggg caaagccatc
    34141 tggcacccat caactacatg cgtcgccaga cgctgaaaaa cgccgagcgc tacatcattc
    34201 cagagctaaa agagtacgaa gataaagttc tcacctcaaa aggcaaagca ctggctctgg
    34261 aaaaacagct ttatgaagaa ctgttcgacc tgctgttgcc gcatctggaa gcgttgcaac
    34321 agagcgcgag cgcgctggcg gaactcgacg tgctggtgaa cctggcggaa cgggcctata
    34381 ccctgaacta cacctgcccg acctttattg ataaaccggg cattcgcatt accgaaggcc
    34441 gccatccggt ggttgaacag gtgctgaatg agccgtttat cgccaacccg ctgaatctgt
    34501 cgccgcagcg tcgcatgttg atcatcaccg gtccgaacat gggcggtaaa agtacctata
    34561 tgcgccagac cgcgttgatt gcgctaatgg cctatatcgg cagctacgta ccggcgcaaa
    34621 aagtcgagat tggcccgatt gaccgtatct ttacccgcgt aggcgcggca gatgacctgg
    34681 cttccgggcg ttcgaccttt atggtggaga tgaccgaaac cgccaatatt ttacataatg
    34741 ccaccgaata tagtctggta ttgatggatg agatcgggcg tggaacgtcc acttacgatg
    34801 gtctgtcgct ggcgtgggcg tgcgcggaaa atctggcgaa taagattaag gcattgacgc
    34861 tgtttgccac ccactatttc gagctgacac agttaccgga gaaaatggaa ggcgtcgcca
    34921 acgtgcatct cgatgcactg gagcacggcg acaccattgc ctttatgcac agcgtgcagg
    34981 atggcgcggc gagcaaaagc tacggcctgg cggttgcagc tctggccggc gtgccaaaag
    35041 aggttattaa gcgcgcacgg caaaagctgc gtgagctgga aagcatttcg ccgaacgccg
    35101 ccgctacgca agtagatggt acgcaaatgt ctttgctgtc cgtaccagaa gaaacttcgc
    35161 ctgcggtcga ggcactggaa aacctggacc cggattcact gactccgcgt caggcgctgg
    35221 agtggattta tcgcttgaag agtctggtgt aataaaaatt cccgatagtc ttttgctatc
    35281 gggaatatta acgacaactg acgaatcaaa taaaaatacc ctgtataata ggaaagctta
    35341 ttttacaggg taaaaccatg ccatctacac gctatcaaaa aatcaatgcc catcactatc
    35401 gccatatatg ggtcgttggt gatattcatg gtgaatatca gttattacaa tcccgcttac
    35461 atcaactctc ttttttcccc gaaaccgact tacttatttc tgtcggcgat aatattgatc
    35521 gtgggccgga gagtcttgac gtcctgcgcc tgctaaacca gccctggttt atctccgtta
    35581 aaggcaacca cgaagcaatg gcgctggatg cgttcgcgac cggcgatggc aatatgtggc
    35641 ttgccagcgg tggtgactgg tttttcgatt taaatgattc agagcaaaaa gaagctacag
    35701 atctgttgct gaaattccat caccttccac atattattga aatcactaac gacaacataa
    35761 aatatgtcat cgcacatgca gattatccgg ggagtgaata tctctttggt aaagaaatag
    35821 cggagagcga attactctgg cctgttgatc gtgtgcagaa atcgcttaat ggcgagttac
    35881 aacaaataaa cggcgctgat tattttatat ttggacatat gatgtttgat aacattcaga
    35941 cgttcgctaa ccagatttat attgataccg gatcgccgaa aagtgggcgg ctgtcatttt
    36001 ataaaataag gtagtctcat gcttcttctg tgaagcatga gtaaccagat gttattgcag
    36061 gccattattc atttttcgct accagcaaag agagatcctg cttcgccagc gcgcgactgg
    36121 cactctccgg caaaccgtcg tctgtaataa tctgatcaaa ctcgcttaat ggtaacgcca
    36181 gccatgtcgc cacctgacca tatttcgtcg catcacagac caaaactcgc tggcggctgg
    36241 cactggcaat cgcccgtttc accgtgactt tatcttccgc tggcgtagaa atcccccgca
    36301 cactccatga cgatgcagaa ataaaagcct gatcaatcat caggctgcgc agcatggtcg
    36361 cagcggcttc cccgacacag gaacggtttt cccgacacac tgcaccgcca gtgtgaataa
    36421 ttgtgcaatt actgttgtcg agcaagtagt tcgcaataac gaaatcgttt gtgaccacag
    36481 tcagtgactc catgtgaatc agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca
    36541 gatagataca acttcccggc tgaacaagac ttgccgccag cttgccaata gccgcttttt
    36601 gcgtcattgc cagcgcagtt tttacctgat gagaaggttc atgcgccacg cgtcccggag
    36661 actggacgcc tccggacacc agcacaacgg ctccctgctg ctccagtttt tgtaaatccc
    36721 gacgaatggt catatgtgac acattcattc tgtccgttag ttcagcaata ctgacaatgc
    36781 ctttttcagc taccatctca aggatgattt ggcgacgctc tacgggtatc aacttttgct
    36841 ccttcctttg tcctgctgac attctacgct atttgcctgc gaaacgtgcg cggcgcaact
    36901 aacgcttagt tcacataaaa taacacacaa tgttaattta tgtgaatcag ctcaccatat
    36961 cgttatcttc cagcgcttat attcacaata tcaaacaaaa tatcacttaa attaacaagg
    37021 agagcagatg aaaacgggat ctgagtttca tgtcggtatc gttggcttag ggtcaatggg
    37081 aatgggagca gcactgtcat gtgtccgcgc aggtctttct acctggggcg cagacctgaa
    37141 cagcaatgcc tgcgctacgt tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc
    37201 gacgtttgcc gaaaaactgg acgctctgct ggtgctggtg gtcaatgcgg cccaggttaa
    37261 acaggtgctg tttggtgaaa caggcgttgc acaacatctg aaacccggta cggcagtaat
    37321 ggtttcttcc actatcgcta gtgctgatgc gcaagaaatt gctaccgctc tggctggatt
    37381 cgatctggaa atgctggatg cgccagtttc tggtggtgca gtaaaagccg ctaacggtga
    37441 aatgactgtc atggcctccg gtagcgatat tgcctttgaa cgactggcac ccgtgctgga
    37501 agccgttgcc ggaaaagttt atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa
    37561 aattattcac cagttgttag cgggcgtaca tattgctgcc ggagccgaag cgatggcact
    37621 tgcagcccgt gcggggatcc cgctggatgt gatgtatgac gtcgtgacca atgccgccgg
    37681 aaattcctgg atgttcgaaa accggatgcg tcatgtggtg gatggcgatt acaccccgca
    37741 ttcagccgtc gatatttttg ttaaggatct tggtctggtt gccgatacag ccaaagccct
    37801 gcacttcccg ctgccattgg cctcaacagc attgaatatg ttcaccagcg ccagtaacgc
    37861 aggttacggg aaagaagacg atagcgcagt tatcaagatt ttctctggca tcactctacc
    37921 gggagcgaaa tcatgatcaa gattggcgtt atcgccgatg attttaccgg cgcgacggat
    37981 atcgccagtt ttctggtgga aaacggtcta ccaacggtac aaattaacgg tgttccaaca
    38041 ggtaaaatgc cggaagcaat cgacgcactg gtgatcagcc tgaaaacgcg ctcctgtccg
    38101 gtggttgaag ccacacagca atcgctggcg gctctgagct ggttgcaaca gcaaggttgc
    38161 aaacagatct atttcaaata ctgctctact ttcgacagta cggcgaaagg taatatcggc
    38221 ccggttaccg atgcattaat ggatgctctc gacacgccgt ttacggtctt ctctccggcc
    38281 ctgccggtca acggacgtac ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg
    38341 gccgaatccg ggatgcgcca tcacccggta aatcccatga ccgacagcta tcttccccgt
    38401 ctggttgaag cgcaatccac agggcgctgc ggcgtcgttt cggcacatgt tttcgaacaa
    38461 ggtgtggatg ccgttcgtca agagctggct cgcttacagc aagagggcta ccgctacgcg
    38521 gtgcttgatg cgctgaccga acaccatctg gaaattcagg gagaagcctt gcgcgatgcc
    38581 ccactggtaa cgggcggttc tggtctggcg attggcctgg cccggcagtg gacgcaagaa
    38641 aacggtaacc aggctcgcaa agcagggcat ccgctcgctg ggcgcggcgt agtgctctcc
    38701 ggttcatgct ctcaaatgac caaccgccag gtagcacatt accgtcaaat tgcaccagcc
    38761 cgtgaagttg atgtggcacg ctgcctctca actgaaactc tggctactta tgcacacgaa
    38821 ctggcagagt gggttctggg ccaggaaagt gtacttgctc cactggtttt tgccaccgcc
    38881 agcactgacg cattggcagc aattcaacag caatacggtg cacaaaaagc cagtcaggca
    38941 gtagaaacac tgttttctca actagcggcg cggttagcgg cggaaggcgt ggcacgcttt
    39001 attgtcgcag gcggtgagac ctccggcgta gtcacacaga gcctgggcat aaaagggttt
    39061 catattggcc caaccatttc ccccggcgtg ccgtgggtaa acgcactgga taagcctgtc
    39121 tcactcgccc ttaaatctgg caacttcggt gatgaagcct ttttttcacg agcccaaaga
    39181 gagtttttat catgagcgat ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc
    39241 ggattgccag ttcattcttt cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc
    39301 tgcttttacc tgacgggaat ttactggcga caccgacagg ttcatgcctg ggcaatctcg
    39361 atccgcagcg gctttccaaa gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct
    39421 cgaaagaggt gctctttcat ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg
    39481 tgcatttgca cagcacatgg tcgacggcgc tttcctgcct gcaagggctg gacagcagca
    39541 acgttattcg tccgttcaca ccatacgtgg tgatgcggat gggaaatgtc ccgctggtgc
    39601 cttattaccg accgggcgat aaacgcatcg cacaggatct ggcggaactg gcagcagaca
    39661 atcaggcttt tttactggca aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag
    39721 ccgccaacaa tatggaagag ctggaggaaa cggcaaagct gatttttatt ctcggtgacc
    39781 gcccgatccg ttatctgacc gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct
    39841 cgttttgcag ctaatttatc catgatgttc accgaagtgc cttttattga acgcttcgcc
    39901 gcagcgcgaa aagccgggtt cgatgctgtg gaatttctgt ttccctatga ctactccacc
    39961 ctgcaaatcc aaaagcaact ggagcaaaac catctgacac tggcgctgtt caataccgct
    40021 cccggagata ttaatgcggg ggaatgggga ttatccgccc ttcccggacg tgagcacgaa
    40081 gcacacgcag acatcgacct ggcactggaa tatgcattgg cgctcaattg tgaacaagtc
    40141 catgtgatgg caggcgtcgt gcccgctggc gaagacgctg aacggtaccg ggcagtattt
    40201 atcgataatc tccgctacgc tgccgaccgc tttgcgccac acggcaagcg aatattagtt
    40261 gaagcactca gccccggcgt taagcctcat tatctcttct ccagccagta tcaggcactg
    40321 gctattgttg aagaggttgc gcgagataac gtgtttattc aactagatac ttttcatgcg
    40381 caaaaagtag atggcaacct gacgcattta atccgcgact acgctgggaa atatgcgcac
    40441 gtacaaattg ccggactacc tgatcgacat gaaccggacg atggagaaat caactatccg
    40501 tggctgttcc gcctgttcga tgaggtggga tatcagggct ggatcggttg tgaatataaa
    40561 cctcgtggcc tcaccgaaga agggcttggc tggtttgacg cctggcgcta attcgttaaa
    40621 tccctaatta caacgtaccc atacatcccc ccatctacgg atggggtagg ttaactataa
    40681 ctttcagaca gggtttccca tgtccacaat tacattgtta tgcattgcgt tagctggcgt
    40741 aatcatgctg ttgctgctgg tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct
    40801 cgtcagcctg ttagtcgcac ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat
    40861 gatcgccggg atgggcggtg tgcttggttc cgtcactatt attattggtc tgggcgctat
    40921 gctggggcgt atgatcgaac actctggtgg tgcagagtca ctggctaatt atttcagtcg
    40981 caagttaggt gacaaacgaa ctatcgctgc gctgactctg gcagcgttct tcctcggtat
    41041 tcccgtcttc tttgatgtcg gctttattat tcttgcgcca atcatttacg gttttgccaa
    41101 ggttgccaaa atatcgccac tcaaatttgg cctgcctgtc gctggaatca tgctaactgt
    41161 tcatgtggcg gtaccgccgc atccaggccc tgtcgccgca gcggggttac tccacgcaga
    41221 catcggctgg ctaaccatca tcggtattgc gatttctatt cccgtaggga ttgttggcta
    41281 ctttgcagcg aaaataatca atacgcgcca atatgcgatg tcggtggaag tactggaaca
    41341 gatgcaactg gctccggcca gtgaggaagg cgcaacaaaa ttaagcgata aaataaatcc
    41401 accgggcgtc gcgctggtca cctcgctaat tgttattcct atcgcgatta tcatggcggg
    41461 tacggtttcc gcaacactga tgccgccttc gcatcccctg cttggtacgc tacagctgat
    41521 cggctcacca atggtagctc taatgattgc gctggtgctg gcattctggt tattggcttt
    41581 acgtcgcggc tggagcttac aacacaccag cgacattatg ggctcagccc ttcccactgc
    41641 ggcggtagtg attttggtta ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc
    41701 gggcgtcggc aaagcccttg ccaatatgct acaaatgatt gacctgcctc tgttaccagc
    41761 cgcatttatt atttcactgg ctctgcgtgc atcgcagggg tcagcaaccg tagcaatcct
    41821 gacaaccggc gggttactct cagaagcggt gatgggattg aatccgattc agtgcgtatt
    41881 ggtgacgctg gcagcctgct ttggtgggct tggcgcgtca catattaatg actcagggtt
    41941 ctggattgtg accaaatatc tggggttgtc ggtagcagac ggtctgaaaa cctggacagt
    42001 gttaacgacc attctcggtt ttaccggatt cttaattacc tggtgcgtat gggcggtaat
    42061 ttgaccttaa taaaaaggtc cgatgggcat cggacctttt atttaacgct taccttccac
    42121 cagcagcggc gggatgctcg gcaccattgg cgcgtcatca atatctttct gcgtcatgcg
    42181 gaacgcttcg ggataatgtt cgcggctggt acggcgcagc ggttcggtat cgcgcagcgg
    42241 ttcggtatcg cgccaggtat aaaggcaatg ctggcactga tataccgtcc agacatcttt
    42301 caccggcgat ttcgccatca cttcaatctg ttcatcggca caacgtggac aaatcatctt
    42361 cttctcctta tttacgtgca gccagcatag cggtcagttt ttcagcccag gctttggttt
    42421 ccggtaaatc caccaccggt tggctgtagt gaccacggtt gtccggggcg acaggcgtag
    42481 tggcgtcgat aatcagcttg tcggtgatcc ccgcagggct tgagcctgga tcgagttcca
    42541 gcacggacat attcggcaac tgcaccaaat cccctgccgg gttcactttc gaggagagcg
    42601 cccacatcac ctgcggcagg ttgaacgggt caacgtcttc atcgaccata atcaccatct
    42661 tcacgtagcc cagaccatgc ggcgtggtca ttgcgcgcag gcccaccgcg cgggcaaagc
    42721 cgccgtagcg ttttttggtg gagataatcg ccagcaggcc atgggtgtac atggcgttta
    42781 ccgcctgcac ttcagggaac tcggctttca gctgctgata cagcggcacg caggtggctg
    42841 gccccatcag gtagtcgatt tcggtccacg gcataccgag gtacagcgat tcgaaaatcg
    42901 gcctggtgcg gtaagagact ttatcgatgc gcaccacggt catgttacgc ccgccggagt
    42961 agtgcccggt aaactcaccg aacggccctt cgatttcgcg tttacggctt tcgatgaccc
    43021 cttcgaggat cacttctgaa ccccacggca catcaaaacc ggtcaacggg gcggtggcga
    43081 tcgggtacgg gctttcacgc agcgcgcctg ccatttcgta ctcggactga tcatatttca
    43141 gcggcgtggc ccccatcagc gtgatgatcg gatcgttacc gagcgtgatc gcaatcggca
    43201 gatcttcacc gcgctcttct gctttatgca gatgcagggc gatatcgtgc atcggcaccg
    43261 gttgcaggcc gagcttacgc ttgcccttca cttccatgcg gtagatgccg acgttctgct
    43321 tgccgaagtt atccgggtcg agcggatcgc gggaaaccac gcacgctttg tcgagataga
    43381 aaccgccatc gccatcgttt aaacgaaaca gcggcaggat atcgaacagg ttaatctctt
    43441 cgccatcgac ggtgttctgc gcccaggctg gattggcgcg gcgctccggg gcaatcggga
    43501 agttatccca gcggcggata aactcatcaa tctgcttttt gaccggggtg tttggtggca
    43561 ggccaaggga aatcgcgtgg ttctgccagg aaccgatggt attcatcgtc acgcgggcat
    43621 cggtaaagcc gcgaatatta tcaaaccaca gcgcgggcgc gccgtcgccg atacgcccgg
    43681 tggcgttagc tgctgctgcc agatccggct cagcgttcac ctcttcgctg attttcagta
    43741 actggccgtg gtcatcaagc gcctgtaaaa agctgcgtaa atcatcaaat gccattattc
    43801 attctcctga gaaaaattcc gggcctgcgg caatccttgc cagcgcctgg cgtggggatg
    43861 ttcgaggcca aattgatcca gcacgcgggc taccacatgg tggacaatgt catctaccgt
    43921 ttcgggatgg ttataaaagg caggcatcgg cggcaccatc gccacgccca tgcgtgaaag
    43981 tgcgagcata ttttcgagat ggatggtgct aagcggcatt tctcgcggca ccagcaccag
    44041 tttgcggcct tctttgagca cgacgtccgc cgcgcgccct accaggccat cggcgtaacc
    44101 agcgcggata ccggcgagcg ttttcatact gcacggaata acgatcatgc cgtcggtacg
    44161 aaaagaaccg gatgagatga tcgccgcctg atccgccggg ttatggctga agtctgcgag
    44221 ggcagcaaca tcgcgagcgc tgtaaggcgt ttccagttca atggtggttt tcgcccactt
    44281 cgacatcacc agatgagtct cgacattcgg catctcccgc agcgcttgca gtaatgccac
    44341 accaagaggc gcaccggtag cccctgtcat cccgacgatc agtttcattg cttatcctct
    44401 taattgttcg tatacgaacg ttattattaa catactcctt catttccaca gcggcaagga
    44461 ttgaaaagta acaacatctt cttcgtcact aaaaaaccgc tatgatagca acagagaatt
    44521 agcccggagt ttacatggcg ttacgaaata aagcgttcca tcagttacga cagctttttc
    44581 agcagcacac ggctcgctgg cagcacgagt tacctgacct gaccaaacca cagtacgcgg
    44641 tgatgcgcgc cattgctgat aaacctggta tcgaacaggt ggcactgata gaagcagctg
    44701 tcagcaccaa agcaacgtta gcagaaatgt tggcaagaat ggaaaatcgt ggcttagtca
    44761 gacgtgaaca tgaccccgct gataagcggc gacgctttgt ctggttgact gccgaaggcg
    44821 aaaagatact tgctgcggcg atacccattg gtgacagcgt ggatgaggaa tttttggggc
    44881 gtttgagtgg agaagagcag gagctgttta tgcagctggt gcgcaagatg atgaacgcat
    44941 agggggcaaa tttccggatg cgacgctatc gcgtcttatc cagactacaa atacaccgac
    45001 tcaccaaccg aaagaaaaaa ggccagcctc gcttgagact ggcctttctg acagatgctt
    45061 acttactcgc ggaacagcgc ttcgatattc agcccctgcg tttgcaggat ttcgcgcaaa
    45121 cggcgcaggc cttcaacctg aatctggcga acacgttcac gggtgaggcc aatttcacga
    45181 cctacatctt ccagtgttgc cgcttcgtac cccagcaaac cgaatcgacg tgccagcact
    45241 tcacgctgtt tggcgttcag ctcgaacagc catttgacga tgctctgctt catatcgtca
    45301 tcttgcgtgg tatcttccgg accgttctct ttttcatcgg ccaggatgtc cagcaacgct
    45361 ttttcggaat caccacccag cggggtgtct accgaggtaa tgcgctcgtt aagacgaagc
    45421 atacggctga cgtcatcaac tggcttatcc agttgctctg cgatctcttc cgcacttggt
    45481 tcatggtcca gcttatggga caactcacgt gcggttcgca ggtaaacgtt cagctccttt
    45541 acgatgtgaa tcggcaaacg aatagtacgg gtttggttca taatcgcccg ttcaatcgtc
    45601 tggcgaatcc accaggttgc gtatgttgag aagcggaaac cacgttccgg gtcaaacttc
    45661 tctaccgcgc ggatcagccc caggttgccc tcttcgataa ggtccagcaa cgccagacca
    45721 cgattgccat aacggcgggc aatttttacc accagacgca agttactctc gatcatccgg
    45781 cggcgagagg cgacatctcc acgcagtgcg cgacgcgcaa aataaacttc ttcttcggcc
    45841 gttaacagtg gtgaataacc aatctcacca aggtaaagct gagtcgcgtc caacacacgc
    45901 tgtgtggctc cctgcgataa cagttcctct tcggccaaat cgttatcact gggttcctct
    45961 tctactaagg ccttttcgtc aaaaacctca actccgttct catcaaattc cgcatcttca
    46021 tttaaatcat gaactttcag cgtattctga ctcataaggt ggctcctacc cgtgatccct
    46081 tgacggaaca ttcaagcaaa agcatggttc cgccgattta tcgctgcggc aaataacgca
    46141 gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg caagcgtgtt gaactggttc
    46201 cggtgctacc catggttgct attttttgcc ccgccttcac ttcttgttgt tcccggacca
    46261 gcattgtgtc gttatgggcg taggcactca ggtaatcatc attatgtttg atgataatca
    46321 gattaccgta gccgcgcagc gcgttaccag cataaacaac gcggccatct gcggtcgcga
    46381 taattgcctg tcctttgctg cctgcgatat caatcccctt gttgcccccc tcagaagcgc
    46441 caaaggtttc gatcactttg ccctcagtcg gccagcgcca ggtggagata ggcgtactgg
    46501 ttgatgtact gctgacagtt ggctcggttg tgcttgctgt tggtaccgtt acaggcgctg
    46561 tgaccgtggt cgcagctggc ttgttgttcg gcaacatttt gttagcactc tgttcacccg
    46621 aagattcaga atacgtaatt gtcggttgcg acgcaacagc aacggtggaa ttttgtgcag
    46681 gcttgatcac aactccttgc tctgctgcgt cggcctgggt aatggcattt ccgccagtga
    46741 ttggcgtacc ggaagcatta cccacctgca aggtctgacc aacgttcagc gcgtatggtg
    46801 cctgaatatt gttgcgctga gcaaggtcac ggaaatcgtt gccagtaatc caggcgatat
    46861 agaaaagtgt gtcgcctttt ttcacggtat aggtactgcc gctataactg cctttcggaa
    46921 tgttcccata ctgacggtta tagacgatgc gtccgttttc catctgtacc ggctgctgag
    46981 ctactggctg cactggctgg atttgcggtt gttgagtagc ctgaatttgt ggctgctgta
    47041 ccggctgaat ttgcggttgc tgggctgtag acgtcgtccc cattttcggc ggcggcgtaa
    47101 tcaacatacc agaattggta tttgcaggcg cattgccatt aacggagctg accggtgccg
    47161 gtggatttga agtgtcagaa cagcctgcca gccatagcga aaccagtgac aaagccgcaa
    47221 tgcggcgaac ggtgaatttt gggcttcccg cgctcattta tcccccagga aaaattggtt
    47281 aataaccagt gacataatta ccgtgcaagg caccctactg aacactggaa aagatgttca
    47341 cgatacgctg acctgcggca aaataaccag gaaaaatcca ggtatttcct cacgttttaa
    47401 gccagctcac ccttcactaa agggacaaag cgcacggcct ccacggtatc gataataaat
    47461 tcgcctcccc gacgacgcac ccgtttcaaa tactggtgct cctcccctac gggtaagacg
    47521 agaatcccgc cttcgtccag ctgcgtcatt agcgcagttg gaatttccgg cggtgccgcc
    47581 gtaacaatga tagcgtcaaa cggcgcgcgt gcctgccaac cttgccatcc atcgccatga
    47641 cgggttgaaa cattatgtaa atcaagattt ttcaggcggc gacgcgcctg ccactgcaaa
    47701 cctttaatcc gttcgaccga gcaaacatgc tggacaagat gcgccaggat tgccgtttga
    47761 tatcccgaac cagtgccaat ttccagcacc cgcgactgcg gcgtcagctc gagtaattcg
    47821 gtcattcgcg ccaccatata cggctgcgaa attgtctgcc cctgacctat cggcaaggcg
    47881 atattgtccc aggctttttg ttcaaacgct tcatcaacga atttttcacg cggcacggcg
    47941 gcaagtgcat tcagcacctg ctcatcctga ataccttgcg cacgtaattg atccagaagt
    48001 gcttgtacgc gtctgcttac cattgcgtgc caactcccac gctgtttaac cagtctgaaa
    48061 ccacatcttg cgcgctatgc gcagttaaat ccacatgcag cggcgtgatg gagacatagc
    48121 cctcatctac cgcagcaaaa tcggtccccg gaccggcatc acatttaccg cccggcgggc
    48181 caatccagta cagcgtattg ccgcgcggat cttgctgcgg gatcacctga tctgccggat
    48241 gtcgtgtacc gcagcgcgtc acgcgaatac ctttgatttg atccaagggt aaatccggaa
    48301 cgttaatatt aagaatacgc ccggtgcgca acggctcttt acacagtgcg cgcaaaattg
    48361 aacaggttac cgccgcggca gtgtcgtaat gtttatgccc gtcaagcgag acggcaagcg
    48421 ccggaaaacc taaatgacgg ccttccatcg cggcggctac cgtaccggaa taaataacgt
    48481 catcccccag attcggcccg gcgttaattc cggacacaac aatgtccggg cgcggacgca
    48541 tcagagcatt cacgccaaga tagacgcaat cggtcggggt tcccatttgc acagcaatat
    48601 caccattttc aaaggtaaac gtgcgcaggg aggattccag tgtcagagaa tttgaagcgc
    48661 cgctgcggtt acgatcgggg gcgaccacct gaacgtcagc aaactcacgc aaggctttcg
    48721 ccagcgtttg tataccgggt gcatgtaccc cgtcatcatt actcagcaat atgcgcataa
    48781 tcacctgttg tgttgataag ttccctgaca acgctggttg caaaactacc cgccggaagc
    48841 cagaaacgga tctctacggt gacgtcatcc caccaattcc agcttaattg ttgcggatac
    48901 agcaacatcg ctctgcgcgc ggcttcaact ttttcgcgca ccagtaaagc ttgtaattca
    48961 gtttctgcgg cgacagctgc cttttcgaat gccagcgctt cacgctgagt tccccattcg
    49021 ccactgcccg gcaatgcggc ggttatcatc agctctttat cgttgacgcg acgctgtaat
    49081 tccgccagtt cttcggtggt tgctacaaac cagctaccac gtccggctaa ttgtagcgca
    49141 tcgccgtcaa caacttgatt aacgtctgct tttttgaggc gctcagcaac aatctgatta
    49201 aacaacgcac tgcgggctgc cgacaaccaa aaactccgtt tattgcgatc gcgcaccgga
    49261 gtattggttt gcgcccagcg cagcgcgccc tgcaagttgc taccgccaat cccaaaacgt
    49321 tgggcaccga agtagttcgg tacacctttt acgcaaatat cgatcagacg ttgttcaacg
    49381 tcatcgcgat tgctcacttc gcgcagaacc agggtaaagg cgttaccttt cagcgcgcct
    49441 aaacgcagct tgcgcttgtg ccgcgcatac tccagcacct ggcagccttc cagttgaaag
    49501 gcgctcagat cgggcatttc cttgcctggc acgcgagcgc ataaccactg ttccgtaaca
    49561 gcatgtttgt ctttttgccc agcgaagctg acttcacggg catgaatttt caggaatttc
    49621 gccagtgcat ccgccacaaa acgggtattg cagccgtttt tgaggattct aaccagaata
    49681 tgctcacctt caccatcagg ctcaaagccc aaatcttcca ccaccacaaa gtcttccgga
    49741 ttggctttca gcagcccggt gccttgcggt ttaccgtgga ggtaagtgag attatcaaac
    49801 tcaatcattt tgttgcctta atgagtagcg ccaccgcttc acaggcaatc ccttccccac
    49861 gtccggtaaa tccaagtttt tccgtagtag tggctttcac gttaacatca tccatatggc
    49921 agccgagatc ttcggcaata aacacgcgca tttgtggaat gtgcggcaac atcttcggtg
    49981 cctgagcgat gatagtgaca tcgacgttgc ccagggtata acccttcgcc tgaatacgac
    50041 gccaggcttc gcgtagcagc tcgcggctgt cggcaccttt aaatgccgga tcggtatccg
    50101 ggaacagctt gccgatatcc cccagcgccg ccgcgccaag caatgcatcg gtcaacgcat
    50161 ggagcgccac gtcgccatca gaatgcgcca gcaatccttt ttcgtaagga atgcgtacgc
    50221 caccaatgat aattgggcct tcaccgccaa aggcatgtac gtcaaaaccg tgtccaattc
    50281 gcattatgta ttctcctgat ggatggttcg ggtgaggtaa aactcggcca gtgccaaatc
    50341 ttccgggcgc gtgactttaa tgttatccgc acggccttcg accaactgag gatggaatcc
    50401 gcaatattcc agcgccgagg cttcgtcggt aatagtcgcg ccttcattta gagcgcgcgt
    50461 cagacagtca tgtaacagct cacgagggaa aaattgcggc gtcagcgcgt gccataagcc
    50521 gttgcgatca acggtatgag caatggcatt tttgcccggt tcggcacgtt tcatcgtatc
    50581 gcgcactggt gcggctagga tccctcccgt gcggctggtt tcgctcaacg ccaacaatcg
    50641 cgcgaggtca tcctgatgca gacaaggacg agcggcgtca tgcaccaata cccactgcgc
    50701 gtcgccagcg gctttcaaac ctgccagcac ggaatcggca cgctcatcac cgccatctac
    50761 aacggtgatt tgcggatgat tcgccagagg aagttgtgca aaacggctat cgccaggact
    50821 tatggcaatg acgacacgtt tcacccgggg atgcgccagc aacgcatgca ccgagtgttc
    50881 aagaatggtt tgattaccga ttgagagata ttgcttagga cattccgttt gcattcgacg
    50941 gccaaatccg gccgccggaa ccacggcgca aacatccaaa tgagtggttg ccatgttaat
    51001 tcccgggctg atttatcgat tgttttgccc cgcagactgt gcgcgcttcg acgcgtcagg
    51061 caccagacga taaaaagttt cgcccggcct ggtcatgctg agttcattac gcgcacgctc
    51121 ttcgagcgcc tcctggccgc cattgagatc gtcaatttcg gcaaaaagtt gatcgtttcg
    51181 cgctttaagt ttcgcgtttg tagcttgctg tgccgccaca tcatcattga cgcgggtata
    51241 gtcatgtata ccgttcttac cgaaccacag cgaatactgt agccagacca gaatagccag
    51301 caacagcagc gttagtttac ccatcctgcc ccctgaaaaa cggcatcatc atcccatgca
    51361 tccgaagacg actctacatc ctctgttggg gataccgcga caacgcgggc aaatgtacca
    51421 catttgtcca ttgttacgta tacccagggc gtgcagaaca taatctcatt attagttacg
    51481 gtttgaatta tgaacagagg agacgggaaa gtacaaatta gcccagtagc cacataaaca
    51541 gtgcgccaaa cataatgcct actgtcacca gggtgaaaac aatactgtag cgtagctttc
    51601 cgtccatcaa tgaatgcagc gcaatcccca ccactaccgc gacgggcatc agcgccagaa
    51661 agaaaggcca ggtgtagata aagaagaaca gcgtgttaga gccataaatc aacatcggca
    51721 tcgccagcgc aaataaccag gagataaaac cgaccacggc accaggcagt gaccatgtgg
    51781 tttcttcatc ctcagtaagg ctgtcgttat ttgttagtgt aatgttatgg ctattacgca
    51841 tatttgatcc tgttactttg acgaaccggg catggaaacc cggtggtgtc tcaggatctg
    51901 ataatatcgt tctgtctcaa cagatctaat aattgctgta ccaaatttgt tactaattgt
    51961 tcaccattga gatgaatttc tgccgattca ggcgcttcgt aaacagaatc tattcccgta
    52021 aagttgcgca gttcaccggc acgcgctttc ttatataagc cttttggatc gcgggcttcg
    52081 caaatcgcca gcggcgtatc gacaaacact tcgataaagc gcccttctcc tacgcgttcg
    52141 cgaaccatct ggcgttcggc gcggtgtggc gagataaatg cggtcagcac taccagtccg
    52201 gcttcaacca tcaaattcgc cacttcaccg acgcgacgga tattctcttt acgatcggca
    52261 tcgctaaaac cgagatcgct gcataatccg tggcgaacat tatcgccatc cagcagatac
    52321 gtactgacgc cgagtttatg taacgcctcc tccagcaccc cggcgaccgt tgatttaccg
    52381 gacccggaga ggccggtaaa ccacagcact acaccacgat gaccgtggtg tagctcgcgt
    52441 tgttgcacag tgaccggatg gctatgccag acgacgtttt cgtcatgcag cgccattatt
    52501 tatcccccag caaatcgcgc gcgccccagt gcggaaagtg gcgacgaacc agagcattca
    52561 attccagttc gaatgcactg aattcagatg gcgcagcagt tgcctggcta actggctcgt
    52621 gcaccatacc ggcacccacg gtcacattgc tcaggcgatc gataaaaatc agcccacccg
    52681 tcaccggatt ttgttgataa cgatctaaca ccagcggctc gtcaaaagtg agatccacga
    52741 ggccgatccc attcagtggc aggttttcaa cttcacgctg ggtaaggtta ttaatatcaa
    52801 cctgatagcg aatgccatca acacgcgcgc gcgtcttctt accggcaatt ttgatgtcgt
    52861 aactctgccc tggagaaagc ggctgttccg ccatccatac cacatccacc gacgcgctct
    52921 gcaccgccgg taacgcttcg tctgccgcca gcagcagatc gccacggctg atgtcgatct
    52981 catccgtcag caccagggtg atcgcttctc cggcaaaggc ttcttcgcga tcaccatcaa
    53041 aagtcacgat ccgcgcgacg tttgattcca caccagaagg cagcactttt acacgttgcc
    53101 cgacttccac gcgaccggat gccagcgttc cggcgtaacc acgaaaatcg agattcgggc
    53161 gattaacgta ctgcaccggg aagcgcattg gctgagcatc caccactcgc tggatctcca
    53221 cggtttccag cacttcgagc agtgtcggac cgctgtacca cggcatactt tcactttgcg
    53281 aagccacgtt gtcgccttcc agcgcagaga gcggcacaaa gcgaatatcc agattgccag
    53341 gtaactgttc ggcgaaagtc aggtagtctt tacgaatgcg ggtgaatgtc tcttcactgt
    53401 aatccaccag atccattttg ttgatcgcta cgaccagatg tttgatcccc agcagcgtgg
    53461 agataaaact gtgacgacgg gtttgatcga gcacgccttt acgggcatcg atcagtaaga
    53521 tcgccagctc acacgttgag gcgccggtcg ccatattgcg ggtatactgc tcgtgcccgg
    53581 gggtgtcggc gataataaat ttacgcttct cggtagagaa atagcggtag gccacatcaa
    53641 tagtgatgcc ctgttcgcgc tcagcttgca ggccgtccac cagcagagcc agatccagtt
    53701 tttcgccctg ggtgccgtga cgcttactgt cgttatgcag cgatgagagc tgatcttcgt
    53761 agatttggcg ggtatcgtgc agcaggcgac caatcagggt acttttgccg tcatcgacgc
    53821 taccacaggt cagaaaacgc agcaggcttt tatgttgttg cgcaatcatc caggcttcga
    53881 cgccgccttc attggcgatt tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga
    53941 cgttttttca gctccataga ccccgcctgg tcgcggtcaa tcacgcggcc ctgacgttca
    54001 ctggtggtgg aaaccagcat ctcttcgatg atttccggca gtgtttgtgc atttgactcc
    54061 accgcaccgg tcagcggcca gcagcccagc gtacggaaac gcaccatccg ttttttaatc
    54121 acttcgcccg gttgcaggtc gatacggttg tcatcaatca tcatcaacat accgtcgcgt
    54181 tccagaaccg gacgttccgc agcgagatat agcggaacaa tgtcgatatt ttccagccag
    54241 atgtattgcc agatatcctg ctcggtccag ttagagagcg ggaagacgcg gatgctttcg
    54301 cctttgttaa tttgcccgtt gtagttgtgc cacagctccg ggcgctgatt tttcggatcc
    54361 cagcgatgga agcggtcacg gaaagagtaa attcgctctt tagcgcggga tttctcttcg
    54421 tcacggcgcg caccaccgaa ggcggcatca aaaccgtatt tgttcagcgc ctgtttcagg
    54481 ccttcagttt tcataatatc ggtatgtttc gcgctgccgt gcacgaatgg attaatcccc
    54541 atcgccacgc cttccgggtt tttatgcacc agcagttcgc agccgtaggc tttagcagta
    54601 cgatcgcgga actcatacat ctcgcggaat ttccagccgg tatcgacatg cagcaacggg
    54661 aaaggcagcg tacctggata aaacgccttg cgcgccaaat gcagcatgac gctggaatct
    54721 ttaccgatag agtagagcat caccggattt gagaattctg ccgccacctc gcgaataatg
    54781 tggatgcttt ccgcctccag ttgccgcagg tgagtaagtc gtttttgatc cataaccgtt
    54841 cctttgcaat accgctattt tcttgccatc agatgtttcg actataggga gcgtaagaga
    54901 acgaatgaaa ttaccaatta gaatgagtag ttccttaacg gaataacgat ttggcaaagc
    54961 taatatcaaa aagtgcttaa ggcaccggat ttcgggcgtt taggaagatt tgaaattgtt
    55021 ttagcgcagc ggcagtttca tactatggcg gtaaaaaaat ttgcatggta tttaaggact
    55081 cactatgttt tccgcattgc gccaccgtac cgctgccctg gcgctcggcg tatgctttat
    55141 tctccccgta cacgcctcgt cacctaaacc tggcgatttt gctaataccc aggcacgaca
    55201 tattgctact ttctttccgg gacgcatgac cggaactcca gcagaaatgt tatctgccga
    55261 ttatattcgc caacagtttc agcaaatggg ttatcgcagt gatattcgga catttaatag
    55321 tcggtatatt tataccgccc gcgataatcg caagaactgg cataacgtga caggaagtac
    55381 ggtgattgcc gctcatgaag gcaaagcgcc gcagcagatc atcattatgg cgcatctgga
    55441 tacttacgcc ccgctgagcg atgctgacgc cgatgccaat ctcggcgggc tgacgttaca
    55501 aggaatggat gataacgccg caggtttagg tgtcatgctg gaattggcag aacgcctgaa
    55561 aaatacgcct accgagtatg gtattcgatt tgtggcgacc agcggcgaag aggaagggaa
    55621 attaggcgct gagaatttac tcaagcggat gagtgacacc gaaaagaaaa atacgctgct
    55681 ggtgattaat ctcgataact taattgttgg cgataaattg tatttcaaca gcggtgtaaa
    55741 aacccctgaa gcagtaagga aattaacgcg cgacagggcg ctggcaattg cgcgcagtca
    55801 cggaatagcc gcaacgacca atccgggttt gaataaaaat tatccgaaag gcactgggtg
    55861 ttgtaatgac gcagaaatat tcgacaaagc gggcattgct gtactttcgg tggaagcgac
    55921 taactggaat cttgggaata aggatggtta tcagcaacgc gcaaaaacag ccgcattccc
    55981 tgcgggaaat agctggcatg acgtaagact ggataatcaa cagcatattg ataaggcact
    56041 tcctggaaga atagaacgtc gctgccgtga tgttatgcgg ataatgctac cgctggtgaa
    56101 ggagctggcg aaggcgtctt gatgggtttg aaaatgggag ctgggagttc taccgcaggg
    56161 gcggggaatt ctaagtgata tccatcatcg catccagtgc gcccggttta tccccgctga
    56221 cgcggggaac acaaaaccaa acttctccat aaattccata gccgcggttt atccccgctg
    56281 gcgcggggaa cacgagtcta tcagcgacac taccggcaat agcgacggtt tatccccgct
    56341 ggcgcgggga acacctatag cgccacgttc cgagcgctgc gagctgcggt ttatccccgc
    56401 tggcgcgggg aacacctaat gagtcagttt gaacagatcc ccgacaacgg tttatccccg
    56461 ctggcgcggg gaacacggct ttaaaattgt gttcccgtct ggcgctgggg gtttatcccc
    56521 gctggcgcgg ggaacacgca tccatgccga cgcctttacg tgtgcggggc ggtttatccc
    56581 cgctggcgcg gggaacacgc gcgaatttgt gcgcatgggg cgcatttttg gcggtttatc
    56641 cccgctggcg cggggaacac acgatggcga tgcgtgagaa agggggtcga tacggtttat
    56701 ccccgctggc gcggggaaca cagttcgctg agtagccttt tttctgtgcc taacggttta
    56761 tccccgctgg cgcggggaac acgaggtggc aatacgcgta gatcatttgg tcgtcggttt
    56821 atccccgctg gcgcggggaa caccaaaata ttacgagctt cgtcaggcca tggaccggtt
    56881 tatccccgct ggcgcgggga acacactaag catacataac tgtttttaaa caaatttatt
    56941 ccgcatcaac aatctaccaa ctaaattaaa acatttcctt atttttaaag aacggataac
    57001 ttattgatta tcaacaggaa gaaaagaaac caaacgtaac ccatccaaat ccaccggaat
    57061 acgtctgttt tctccccagg tctgaaattc aaaacccgac tcggtattgg tcgcccaggc
    57121 catcaccaca tttccgcaac cagccagttg ggtaatttgc tgccagatca tctcccgaat
    57181 acgttttgat gtatcaccaa catacacacc ggcacgcact tccagtagcc agattgcgag
    57241 ccgtccacgt aagcgcggcg ggacattttc tgtaacaacc acgaccatgc tcatcagccg
    57301 cgtcctcgat gaccgctatc gcccaacgtt tcaggttcgg ggatggctgg cggtaacata
    57361 tccggcgcag gttgtggcgg ttcaatttca cctgcggcaa ggacttcctc aattaacggt
    57421 attaatttgc ccgttaactt agtgctacgg aaaatatcgc gacaggctaa tctgacttct
    57481 ttatcaggtt ctgcgggttg cctcgctgct atttcaaatg cctttggcac aaccgaatca
    57541 aatttaatga tatcggctat gtcataaaca aatgaaagcg gtttgccact atggataaac
    57601 ccaatagcgg gcgcatatcc cgcggctaat actgccgctt cagatatacc gtacagacat
    57661 gatgtagcag cactgatgca gcgattcaca acatcgcctt tttcccagtc tttaggatcg
    57721 tatttgcgac cattccattt cacaccatat tgtttcgcca gtaatgcata cgtctggcga
    57781 acgcgagatc cctcaattcc acgtaactgc tccactgaac ggcgagctgg ctgtggttca
    57841 cgaaaacgta attcatacat tttgcgcacc accttcaacc gtagatcttc cgttaaagcc
    57901 agctttgcct ggtagagtaa tttatctgcc cgcgccccac cgggttgtcc ggaggagtaa
    57961 acgcgaacgc ccgcttcacc gacccagacc agcagtgttc ccaccgtggc ggccagatgc
    58021 accgccgcgt gggaaactct cgttcctggt tcgagcataa tgcaggcgac cgatcccacc
    58081 ggaatatgcg ttcggatccc tgttttgtcg atcagcacga aagcgccgtc cagtacgtcg
    58141 atctgaccgt actggaggaa gatcatggag gtgcgatctt ttaatgggat cgggctcagt
    58201 ggtacaaacg tcacgcctct gctccgggtt tgatcagcat cagaccacag ccgaacgccc
    58261 ggcttttacc atacccctgg ctcaggcgtt gcacaaataa cccaggatcg gtgaccgtaa
    58321 gcatccccgt ataatccacg ctactgaact ggatcagttg ccgggagttt tcccgccgca
    58381 gttgctgttg tcggtaggca tcaaccgagg tatccagcag tgtaaaaccg cttctctccc
    58441 cctgcgctgc cagccagtcc agcgccgcct gttgttgatg caaccagaca tcacttcctt
    58501 ccgcctgccc cctcacctgc cgtttcgcct ccatcagcag atcgtggcgc ttgcccgctt
    58561 tacagattgt tggattcgcc cgcaggttga aacaaagttg ttgtccggta cacagctcgg
    58621 gcgcaaatga ccggcattct atggtgaacg tatcactttc cgccgggcgt tcctgcgaca
    58681 gtacaaaaaa gcgaaacgcg ccctggagtt cttcccgacg ataaagaaat tgcctttctt
    58741 tgccgccagg gaagagatcc cacagccact gatgcatcac atattcaccg cgatccacca
    58801 gatgcagcaa ctgcgcaggc gaaagctggc cggtatgtaa ggttattctt gagaggtaca
    58861 tggttcctcc ttgctgagcc acggcccctg attgacggtg cgttccccaa acagccactg
    58921 ctgacgattt aaaggaacat cccggcggcg taatattttg ctcgcgacca ggccgtcgtg
    58981 tttcccttcc caccagcatt catcctgaag tttcgggagt gagactttca gtttatggaa
    59041 atgatcctga tactgttggt atgcattaca taaggcatca cacgcgttgc cttcgagcag
    59101 taacggcgca agaggtaatg ccagcggatg actttttcgc cccagataaa gcggaaaaac
    59161 tggatggcgt aaaccgtcct gcaactgttc gaggctgtaa ggcgcatcgg ctgttgttgc
    59221 caccgccacc atccaccagg catcggtgta gtagtcgcgc cgggagataa tcgcgctcaa
    59281 aagctcaggg tcgctcaact cttcgcgacg gctgaaataa cgtgctttac gcacctcttt
    59341 tggcatctgg accgtgtgat aatcccgtgc ccagcgcggg ttacggctgg cgcaaaccac
    59401 cagtgaatag tggcgattaa acgcgtttaa gcgttcggta tcatcacgcc gaatccctac
    59461 cccggcagcc agtagcccca gcaatgctga gcgcgaagga agttcatggg tatgacgaac
    59521 ttcacctgga gcatcgaccc cccaggacgc catcggccca tgaagctgaa aaatcagata
    59581 ttggctcata agccgcccct tacgcgcaga taaagtccag cacgtccttc atgcttccct
    59641 gtttgttcat cacgtcaaaa cttgcgcatt cggtcttctg ttcgtagacc gtattcatgt
    59701 tttcgcgaag cgttgtaata cgctgcaccg ccacatctaa ctgacgtgtg ccattaatgg
    59761 gttcatagaa agccgccgcc agagagcgtg gttgatcggt gcctttttct gccagcgccc
    59821 aggaggcgta ggcacggctg gcaaagctgt tctgtttgcc ggttggggag actttaagtg
    59881 cggcttccgt aaaggcacgt atggtctgat tagctaacgc ttcgtcaccg ccgaggtttt
    59941 ctaccagcag atctttatca atgcagatat aggtgtagaa cagcgcagaa ccgaatccgg
    60001 tttcatctac atgaccggca ccggcatctt cagaagcctg gcgtaaatca tctacggcgg
    60061 tgaaaaaatc attttcgaca atcgtttcac tgacaccaaa cgcatgcgcg acctggcagg
    60121 cggcttctac attaaacccg ggttttttcg ccagcatacg accaaacata gcgatatcta
    60181 ccgccatacg gtctttacgt aacaaagcga gatctttctc ttttggcgcg cgcttttctt
    60241 ctgccagttg atgggccagc gcttttacgg cgtcaaattc tgccgggctg atatggacga
    60301 gctgttcagt ttcggcgcta gtgagcggat cttttggttt tttgtcgttt ttttgttttt
    60361 tgtctttttt ggctttccca agataatcgg ctatctctac tgcccattcg atggcttttt
    60421 tatcttcgat tcctttctca atcaggatag ttgccgcctc acgcgcaata cgcccgctac
    60481 gaacaccaat atggcccgcc agcgcctgtt caaaaagttc agaagtgcgc caagcacgct
    60541 tcagactttg cgaggaaacg cgcagacgcg ttgctccacc cagaatcaca gttttcggag
    60601 agccggaatc atcacggtta agattggcag cagggtaagc ggttaacaaa tgaagctgaa
    60661 taaatgttgt catgagatag tcctttatta attttgttcg ttatcggcgt cgccagcctg
    60721 gtaatattcc agcgcccagc ggatacggat aaattctgtt ggtcgctgct ggcggcggtg
    60781 atgattgagc aggtcatcgc tctcctggca ccagcggaag accccctctg ccagagagtc
    60841 aagattgacg gaaccgttta ataatttgac cgcacggcgt aactgccgca ataaatcatc
    60901 cggcgttttt actgccaaca aacgagtaaa ccgccccttt ttcattaccg ccgccagttg
    60961 cgcggcaaaa ggctgccgtt cgtcaatagc tttaacattg gcgctaactg ctgctatcag
    61021 cgctagtgcg gtgatacgcc actcgggttc atcctgccac tttatttgtc tgttcttaag
    61081 aaacaggcga aatccatccg tcagacaaac atcattaacc gtcgtactac gccgcagact
    61141 ggcacgctcg ccgcgtttat cctgcaactc ttcatgccat ttacgcagcg caactttatg
    61201 ctcatctttt acaatactca ttcagcagcc tcctgctttt tctccctggc ggcgttagca
    61261 ttttgcttct ccgctgatgt tgtaaaatat ttcttgcgtg cggtcatgac gcgttccaaa
    61321 tcaacgggct catatggatt ggtaaagaca cgatcgtcaa aatcctgacg cgcgaataac
    61381 cacatttcct tttgccattt actgagtaat tcgtacggat cctgaccttc ttcaattttg
    61441 cgcactaatc tcaggaatcg atgctgagtt ttgttccaga agtcaatatc cacaaaaccg
    61501 aaatcaagct ttgtggtttt cttctcagag aaccatgctt ctttcaacgc tcggtgtaac
    61561 aagcttagaa tgtgtgatgc cgcttgcgct gccagacgca acttaggtat taggtcttct
    61621 tttttgttta acaacagcgg gaaatggtgt tcataccaac aacgaacctt catatcttca
    61681 aaatcaaaac caaatcccca taagcccacc cttgtctgct ttagattgct ggcattcaaa
    61741 agtttcacga cctgtgcggg gagttcagta ttgttttttg agttacctac ctctatgagg
    61801 cctaaccagt cgcgccagat taaaccacca ggttgtggtt taactgagta aaaatctcca
    61861 ccctctttaa gcggcagacg gtaaggtgtt agaggatggc gccacatgac gtattgaaca
    61921 ccatagtttt tgagtgacat caagcccaaa agagcatcgc tctgcctacc gcaaatgtcg
    61981 cagttaccta ttgaggttgt cttaaaatca atacgtatac gtcgtggcat tccccagtag
    62041 gcctgaagtt tattgacttg ttcatccgtt accacagcac cgtccagttc gctggtacgc
    62101 gtcggtgcaa gccatggaaa gaccaggtca tcaaaggttt ttggtaaagg taaatcggct
    62161 tcatcttgcg gcattacgtt tagccacagc tttcgccaca aaggggtttg ttgattgccc
    62221 tgatactcca gcaattctat aagtgtcgtc agaggccctc cgccacgtag cccaggatag
    62281 taaccgcgtc cacctatagg tgcattcaac tggattgaaa aaagcgctaa aggcacacaa
    62341 tgcaggcaca gatgctgagt tgtgtctcgc ttaataaaat ggtctgtatt tcgttctttt
    62401 gtttgttttc ctggtgcgtc aggaagaagc gaagcaatag aagttgcctt aacttttaat
    62461 tcatcaaaat cctgcataaa tgaaggggaa tctggcccaa actgaaaagc atgttctaaa
    62521 gacagcaatg cttcccttag cttttcagct tccagtccgt cttcccagat atcatcccaa
    62581 cgacgttgat cttttggcgc gaagctggtt tgtagtagcc ccaacaaaaa ctgccaggcc
    62641 gccccctgga gatctgcccg cggcgcagcg atatcgacaa cattttcatc agccagatcg
    62701 actggtgcca gtttgcctgt cgttccgtct ttaaaacgaa cgggcaacca cggggttgtc
    62761 agaagtgaaa acgagttcat acgcatcctc tcgtaaaaaa gaccatccca ggtcggttgt
    62821 ccatccttcc atccttttct gtatatcaac tattcagaga gtcctgtcgc agacatctca
    62881 accaaccttg ccaatcaatc cctccctggc cgaatacccg caactttcgt catcggtgac
    62941 taaaatcacg tttgccattt ccggatcttg ccgctgttca acgcaccact gcctgaacgc
    63001 atccccttcc agtaaagaaa actcatcccg atgttttttc caccagcttc ggcgcactct
    63061 gacaacgctc atttcccatg cgtgagcacc ggtggcataa ggcttcacca caccgtcaat
    63121 acaggtagcc agccacaggg aaacagactc ttcagccaga cgtgtcgaca gtttttccgg
    63181 aaggtaatcg ttgatattgg cggcatagcc gggcctgaag ttcagcacat actttttagc
    63241 ccttgcgcga tcacagtaat atttgcccac ttgctcctgc tcgctgcggg caaatccttc
    63301 cggcattacc acgtcctcac cgtaaactga ttcaataaga agccgggctg cgtgtggcat
    63361 ctgaatagcg ccttgctcac gcagcacacg ctgcgtcagc cagattcgcc catgatcggg
    63421 atagacatat gcactgttac gcatggcact gccgaaccat tcgtcaccag gagcgtcatc
    63481 ccagacgggg gccagaatca gcaattcagg gggggaacgc tcgtcttttc cgtcacgctt
    63541 taactgacca ttaatatcgc ggatatgccg ctgtaatcgc cccgctcgct ggattagcaa
    63601 atcaatgggg gccaagtcgg agatcatttc gtccaggtca atatccacgc tttgttcaat
    63661 tacttgtgta caaacaatga cttgtgacgc tctctgtaaa ctacaatatt taccaaagcg
    63721 tgccagcgtt tccgtttcaa ttcgctggcg atcgctaaaa gcaaagcggc tatgaaaaag
    63781 tgaaaggctg gaagcaggaa tgacgccgcg ggcaagcagc tgacgataca cctgaatagc
    63841 gtcatcaaca gaattccgaa tccaggcgat gcatttcccc tgacttaccg ccgattcgat
    63901 acgcgcaata cactcttgtt cactatggag ccaacccacg ctcacgctac gttcaacttc
    63961 tttgcgcgtc gctacccggt gcgaatgtac atcggttttc gtgacatgcg tcagccaggg
    64021 gtaatcatct tttcccagga gtggggcttc ttgctggccc tctgcaccac gcgcaaaggc
    64081 ggcgacgagt ttgtcgcgct gctgttgaga taacgtagca gaaagcaaaa taacgctgtt
    64141 tccgccacgc gcctgccgct cgatcagccc ttcaagaatg cacgacatgt aagcgtcaca
    64201 ggcgtggatc tcatcagcca gcaggatttt gttactcagc cccagaagcc gcagattatt
    64261 atgtttaaac ggcatcactg ccatcatcgc ctgatccagg gtgccgacgc caatttcagc
    64321 cagcaacgcc ttcttgttac tgtcggcaaa ccaggccgca caaccctggc tgaaagcttg
    64381 ttcatccggt tcttctgacc cgattaaatc accggaccag agtgattcat taaagcggtc
    64441 cattaatgtg cgggcactgt gtgccagcac caggctgggg cgagattctg gcgaatagaa
    64501 agaaagccag gttttgacca gccgatcgta catggcattg gccgttgcca ttgttggcag
    64561 accaaaaaac agcccctgag ctttccccgc agctatcaac ctgtgcgcca ggataagcgc
    64621 cgcttctgtt ttacctgcgc cagtcacatc ttccagaata aataactgcg gtcctggctg
    64681 gctgatatcc agatccagca ccttttgctg taacggtgtc ggattctcaa taaaaggaaa
    64741 cagtgtatta attccggtga aaggtgcggt ttctgctttt ggaggaaaga cggttaatgc
    64801 gttttgagcc tgcactaaag ccttctgcca gtaatgttta atatccattg cctgtgcgac
    64861 gcgtggaaaa aatcgcgttg acgaacctgt ccagtctgcg agtacgacgg ttgcagagat
    64921 ataccaggaa aggtgtttta taagttcaat gccctcgtca tcatcccaga atgcggggat
    64981 ctctattcgc ggaaacagtg ccttgattgc aaggagaaaa tctcgtgctg cagctttgtc
    65041 ttcaggcaga aaattatcca attcatccat acgatcaggg ggccgaccat gatgccctgt
    65101 cgtcacgggc atccacattt ctatcactcg cctaagtttg cgcggagaga gcgaagatga
    65161 aggaagtaac tcctgacatt cacttaaata atgattccac aaccagtagc ccagcgttga
    65221 atgagagatt ttttcgtaat tttttctggc actttcctga atcttgagtt cagggggcag
    65281 gtaaagttgt tgaaaagagc gagcaaattt tccaatatcg tgccagcaca gcaaccaggc
    65341 gaaaaattga gccgcctgtt ccttgtcaga aatccctaat tgacgaaaat aatcagccag
    65401 cccgaagcaa tttcttttaa ccattaaata gcccatcgcg gctacatcca gcgaatgcca
    65461 gcaaagaagg tgatagtcgt cgcccccgtc tttctcgccg cgtcgggttt ttccccagaa
    65521 atcaaaaaaa gtcacaatat ttttatcctt cagtaaactt aaaggatatt tacgca
//
LOCUS       DAZFWP020000003        65351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFWP020000003 DAZFWP020000000
VERSION     DAZFWP020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.003"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..505)
                     /gene="folK"
                     /locus_tag="ACHK0Z_RS00795"
                     /old_locus_tag="pgaptmp_000159"
     CDS             complement(26..505)
                     /gene="folK"
                     /locus_tag="ACHK0Z_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU4500244.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(502..1899)
                     /gene="pcnB"
                     /locus_tag="ACHK0Z_RS00800"
                     /old_locus_tag="pgaptmp_000160"
     CDS             complement(502..1899)
                     /gene="pcnB"
                     /locus_tag="ACHK0Z_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU4500245.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1959..2855)
                     /gene="gluQRS"
                     /locus_tag="ACHK0Z_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             complement(1959..2855)
                     /gene="gluQRS"
                     /locus_tag="ACHK0Z_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU4500246.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2922..3377)
                     /gene="dksA"
                     /locus_tag="ACHK0Z_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             complement(2922..3377)
                     /gene="dksA"
                     /locus_tag="ACHK0Z_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU4500247.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3555..4259)
                     /gene="sfsA"
                     /locus_tag="ACHK0Z_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             complement(3555..4259)
                     /gene="sfsA"
                     /locus_tag="ACHK0Z_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU4500248.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4274..4804)
                     /gene="thpR"
                     /locus_tag="ACHK0Z_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     CDS             complement(4274..4804)
                     /gene="thpR"
                     /locus_tag="ACHK0Z_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU4500249.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4878..7307
                     /gene="hrpB"
                     /locus_tag="ACHK0Z_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             4878..7307
                     /gene="hrpB"
                     /locus_tag="ACHK0Z_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU4500250.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGFLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7503..10037
                     /gene="mrcB"
                     /locus_tag="ACHK0Z_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             7503..10037
                     /gene="mrcB"
                     /locus_tag="ACHK0Z_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU4500251.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10257..12500
                     /gene="fhuA"
                     /locus_tag="ACHK0Z_RS00835"
                     /old_locus_tag="pgaptmp_000167"
     CDS             10257..12500
                     /gene="fhuA"
                     /locus_tag="ACHK0Z_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU4500252.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12551..13348
                     /gene="fhuC"
                     /locus_tag="ACHK0Z_RS00840"
                     /old_locus_tag="pgaptmp_000168"
     CDS             12551..13348
                     /gene="fhuC"
                     /locus_tag="ACHK0Z_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU4500253.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIVQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13348..14238
                     /gene="fhuD"
                     /locus_tag="ACHK0Z_RS00845"
                     /old_locus_tag="pgaptmp_000169"
     CDS             13348..14238
                     /gene="fhuD"
                     /locus_tag="ACHK0Z_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU4500254.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14235..16217
                     /gene="fhuB"
                     /locus_tag="ACHK0Z_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     CDS             14235..16217
                     /gene="fhuB"
                     /locus_tag="ACHK0Z_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU4500255.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16252..17532)
                     /gene="hemL"
                     /locus_tag="ACHK0Z_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             complement(16252..17532)
                     /gene="hemL"
                     /locus_tag="ACHK0Z_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU4500256.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17698..17745
                     /gene="yadX"
                     /locus_tag="ACHK0Z_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             17698..17745
                     /gene="yadX"
                     /locus_tag="ACHK0Z_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU4500257.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17757..19178
                     /gene="clcA"
                     /locus_tag="ACHK0Z_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             17757..19178
                     /gene="clcA"
                     /locus_tag="ACHK0Z_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU4500258.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19202..19267
                     /gene="yadW"
                     /locus_tag="ACHK0Z_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             19202..19267
                     /gene="yadW"
                     /locus_tag="ACHK0Z_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU4500259.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19260..19604
                     /gene="erpA"
                     /locus_tag="ACHK0Z_RS00875"
                     /old_locus_tag="pgaptmp_000175"
     CDS             19260..19604
                     /gene="erpA"
                     /locus_tag="ACHK0Z_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU4500260.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19651..20274)
                     /gene="yadS"
                     /locus_tag="ACHK0Z_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             complement(19651..20274)
                     /gene="yadS"
                     /locus_tag="ACHK0Z_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU4500261.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20312..21112)
                     /gene="btuF"
                     /locus_tag="ACHK0Z_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             complement(20312..21112)
                     /gene="btuF"
                     /locus_tag="ACHK0Z_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU4500262.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21105..21803)
                     /gene="mtnN"
                     /locus_tag="ACHK0Z_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             complement(21105..21803)
                     /gene="mtnN"
                     /locus_tag="ACHK0Z_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU4500263.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21887..23404
                     /gene="dgt"
                     /locus_tag="ACHK0Z_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             21887..23404
                     /gene="dgt"
                     /locus_tag="ACHK0Z_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU4500264.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23534..24958
                     /gene="degP"
                     /locus_tag="ACHK0Z_RS00900"
                     /old_locus_tag="pgaptmp_000180"
     CDS             23534..24958
                     /gene="degP"
                     /locus_tag="ACHK0Z_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU4500265.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25113..26270
                     /gene="cdaR"
                     /locus_tag="ACHK0Z_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             25113..26270
                     /gene="cdaR"
                     /locus_tag="ACHK0Z_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU4500266.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTELDLGNFDDRLLLYVALQLDEER"
     gene            complement(26359..26745)
                     /gene="yaeH"
                     /locus_tag="ACHK0Z_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     CDS             complement(26359..26745)
                     /gene="yaeH"
                     /locus_tag="ACHK0Z_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU4500267.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27057..27881)
                     /gene="dapD"
                     /locus_tag="ACHK0Z_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             complement(27057..27881)
                     /gene="dapD"
                     /locus_tag="ACHK0Z_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU4500268.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27912..30584)
                     /gene="glnD"
                     /locus_tag="ACHK0Z_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             complement(27912..30584)
                     /gene="glnD"
                     /locus_tag="ACHK0Z_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU4500269.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30646..31440)
                     /gene="map"
                     /locus_tag="ACHK0Z_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             complement(30646..31440)
                     /gene="map"
                     /locus_tag="ACHK0Z_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU4500270.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31808..32533
                     /gene="rpsB"
                     /locus_tag="ACHK0Z_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             31808..32533
                     /gene="rpsB"
                     /locus_tag="ACHK0Z_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU4500271.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32791..33642
                     /gene="tsf"
                     /locus_tag="ACHK0Z_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             32791..33642
                     /gene="tsf"
                     /locus_tag="ACHK0Z_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU4500272.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33789..34514
                     /gene="pyrH"
                     /locus_tag="ACHK0Z_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             33789..34514
                     /gene="pyrH"
                     /locus_tag="ACHK0Z_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU4500273.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34806..35363
                     /gene="frr"
                     /locus_tag="ACHK0Z_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             34806..35363
                     /gene="frr"
                     /locus_tag="ACHK0Z_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU4500274.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35455..36651
                     /gene="ispC"
                     /locus_tag="ACHK0Z_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             35455..36651
                     /gene="ispC"
                     /locus_tag="ACHK0Z_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU4500275.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36840..37598
                     /gene="ispU"
                     /locus_tag="ACHK0Z_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /gene_synonym="uppS"
     CDS             36840..37598
                     /gene="ispU"
                     /locus_tag="ACHK0Z_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU4500276.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37611..38468
                     /gene="cdsA"
                     /locus_tag="ACHK0Z_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             37611..38468
                     /gene="cdsA"
                     /locus_tag="ACHK0Z_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4500277.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38480..39832
                     /gene="rseP"
                     /locus_tag="ACHK0Z_RS00965"
                     /old_locus_tag="pgaptmp_000193"
     CDS             38480..39832
                     /gene="rseP"
                     /locus_tag="ACHK0Z_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU4500278.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39862..42294
                     /gene="bamA"
                     /locus_tag="ACHK0Z_RS00970"
                     /old_locus_tag="pgaptmp_000194"
     CDS             39862..42294
                     /gene="bamA"
                     /locus_tag="ACHK0Z_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU4500279.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42416..42901
                     /gene="skp"
                     /locus_tag="ACHK0Z_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42416..42901
                     /gene="skp"
                     /locus_tag="ACHK0Z_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU4500280.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42905..43930
                     /gene="lpxD"
                     /locus_tag="ACHK0Z_RS00980"
                     /old_locus_tag="pgaptmp_000196"
     CDS             42905..43930
                     /gene="lpxD"
                     /locus_tag="ACHK0Z_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4500281.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44035..44490
                     /gene="fabZ"
                     /locus_tag="ACHK0Z_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             44035..44490
                     /gene="fabZ"
                     /locus_tag="ACHK0Z_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU4500282.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44494..45282
                     /gene="lpxA"
                     /locus_tag="ACHK0Z_RS00990"
                     /old_locus_tag="pgaptmp_000198"
     CDS             44494..45282
                     /gene="lpxA"
                     /locus_tag="ACHK0Z_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU4500283.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45282..46430
                     /gene="lpxB"
                     /locus_tag="ACHK0Z_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             45282..46430
                     /gene="lpxB"
                     /locus_tag="ACHK0Z_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU4500284.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46427..47023
                     /gene="rnhB"
                     /locus_tag="ACHK0Z_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             46427..47023
                     /gene="rnhB"
                     /locus_tag="ACHK0Z_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU4500285.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47060..50542
                     /gene="dnaE"
                     /locus_tag="ACHK0Z_RS01005"
                     /old_locus_tag="pgaptmp_000201"
     CDS             47060..50542
                     /gene="dnaE"
                     /locus_tag="ACHK0Z_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU4500286.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50555..51514
                     /gene="accA"
                     /locus_tag="ACHK0Z_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             50555..51514
                     /gene="accA"
                     /locus_tag="ACHK0Z_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU4500287.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51613..53754
                     /gene="ldcC"
                     /locus_tag="ACHK0Z_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             51613..53754
                     /gene="ldcC"
                     /locus_tag="ACHK0Z_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU4500288.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     VQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53811..54200
                     /gene="yaeR"
                     /locus_tag="ACHK0Z_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             53811..54200
                     /gene="yaeR"
                     /locus_tag="ACHK0Z_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4500289.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54265..55563
                     /gene="tilS"
                     /locus_tag="ACHK0Z_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             54265..55563
                     /gene="tilS"
                     /locus_tag="ACHK0Z_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU4500290.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55612..55872)
                     /gene="rof"
                     /locus_tag="ACHK0Z_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             complement(55612..55872)
                     /gene="rof"
                     /locus_tag="ACHK0Z_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU4500291.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55859..56059)
                     /gene="yaeP"
                     /locus_tag="ACHK0Z_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             complement(55859..56059)
                     /gene="yaeP"
                     /locus_tag="ACHK0Z_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU4500292.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56225..56770
                     /gene="yaeQ"
                     /locus_tag="ACHK0Z_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     CDS             56225..56770
                     /gene="yaeQ"
                     /locus_tag="ACHK0Z_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU4500293.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSVFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56767..57189
                     /gene="arfB"
                     /locus_tag="ACHK0Z_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     CDS             56767..57189
                     /gene="arfB"
                     /locus_tag="ACHK0Z_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU4500294.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57203..57913
                     /gene="nlpE"
                     /locus_tag="ACHK0Z_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /gene_synonym="cutF"
     CDS             57203..57913
                     /gene="nlpE"
                     /locus_tag="ACHK0Z_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU4500295.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58068..58891)
                     /gene="yaeF"
                     /locus_tag="ACHK0Z_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /pseudo
     CDS             complement(58068..58891)
                     /gene="yaeF"
                     /locus_tag="ACHK0Z_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58945..60663)
                     /gene="proS"
                     /locus_tag="ACHK0Z_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     CDS             complement(58945..60663)
                     /gene="proS"
                     /locus_tag="ACHK0Z_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU4500296.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60775..61482)
                     /gene="tsaA"
                     /locus_tag="ACHK0Z_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     CDS             complement(60775..61482)
                     /gene="tsaA"
                     /locus_tag="ACHK0Z_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU4500297.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61479..61883)
                     /gene="rcsF"
                     /locus_tag="ACHK0Z_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     CDS             complement(61479..61883)
                     /gene="rcsF"
                     /locus_tag="ACHK0Z_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU4500298.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62001..62816)
                     /gene="metQ"
                     /locus_tag="ACHK0Z_RS01075"
                     /old_locus_tag="pgaptmp_000215"
     CDS             complement(62001..62816)
                     /gene="metQ"
                     /locus_tag="ACHK0Z_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU4500299.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62856..63509)
                     /gene="metI"
                     /locus_tag="ACHK0Z_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     CDS             complement(62856..63509)
                     /gene="metI"
                     /locus_tag="ACHK0Z_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU4500300.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63502..64533)
                     /gene="metN"
                     /locus_tag="ACHK0Z_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     CDS             complement(63502..64533)
                     /gene="metN"
                     /locus_tag="ACHK0Z_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU4500301.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64721..65293
                     /gene="gmhB"
                     /locus_tag="ACHK0Z_RS01090"
                     /old_locus_tag="pgaptmp_000218"
     CDS             64721..65293
                     /gene="gmhB"
                     /locus_tag="ACHK0Z_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU4500302.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15420 a        16274 c        18017 g        15640 t
ORIGIN      
        1 tatttatatt cgtaatataa ataaattacc atttgcttaa tttgtcaaat gctcttgtat
       61 gtaagacttc acgcaacgtc tccccatcag gaaacgccag ctccggtgca atctcaaaca
      121 gcggccacag cataaatcca cgattcttca tatcgtagtg cggaacggtc aggcgttcgg
      181 tatttatcac ttcattacca aacagcatga tatcgagatc cagcgtgcgt ggtccccagc
      241 gttcagcttt acgaacacga ccctgttgca gttctatgcg ctgtgtatgg ttgagtaact
      301 cttccggggc gagcgaagtt tcaagcgcca cggcggcgtt gaggtaatcg ggttgatctt
      361 gcggccccag cggtggggtg cggtaaaacg aagaaacggc aagaatgtgg ctttcaggga
      421 tatcgcctaa tgctttcagg gcagcattga cctgctccag cggagaggcc agattgctgc
      481 ctatggcaat atacgccact gtcatgcggt accctcacga cgcggtgcgc gtttgcgtgg
      541 acgacgagta cgacgacgcg gtgacggttc ttcatccaac tcgttaagca tccctttttg
      601 atctggtggc gcggaaacct ggaactcacc ccaccatttc accagacgct gcagttcagc
      661 gttacgttca acttcagctc gcaaggccaa caggtcataa gccgcacgga acttaggatg
      721 ctccagcagt ttccatgcac gtttaccctg acgtcgggac atacgcaact gcaactgcca
      781 gatatcgcgg gttaacgtcg tcagacgttt cgggattgcc agtgaacggc aggcttcgtc
      841 cagcacgtcg ttcatcgcca gcgcgaaagc gtcgtgatag gtcaggccgc tttcctgggc
      901 gatcttctgt gccgtctcca gcagtgggta ccagaacatg gcggcaaaca ggaacgccgg
      961 gttcacgcgc atatcgttat ggatacgcgt atcggtattc ttcagcacct gttcaatgat
     1021 ccgctccatc gggctgtcgc cattttccgt gaagtagcgg gtaatggtcg ggaacagcgg
     1081 ctggaacaga tgatattcac acagcagctt ataggtttcg taaccgtagc ccgcttgtag
     1141 cagtttaagc gattcttcaa acaggcgtgc cggtgggata tcgttcagca gggtagcgag
     1201 gcgagggatc ggttctgcgg tttccgggct gatgcgcata cccaatttgg cggcaaaacg
     1261 taccgcgcgc agcatacgta ccggatcttc acggtagcgc gtttccgggt taccaatcag
     1321 acggataacg ccgtccttca gatccttcat accgccaacg taatcacgga cggtaaaatc
     1381 cgctacgctg taatacaggc tgttgatagt gaaatcgcgg cgctgggcgt cttcttcgat
     1441 ggagccgaaa atgttgtcgc gcagcaacat gccgttttgc ccgcgttggg aggtcgtgcg
     1501 gtcgctgacg ttaccttcgt ggtgtccacg gaaggtcgca acttcgataa tctccgggcc
     1561 aaacatcaca tgagccagac ggaaacggcg acccaccagg cggcagttac ggaacagttt
     1621 gcgcacctgc tcaggcgtgg cgttagtggt tacgtcaaag tctttcggct ttttgccaag
     1681 taacaggtcg cgcacgccgc cgccaaccag ccaggcttcg tatcccgctt tgttgagcct
     1741 gtacattacc ttcagggcat tttcactgat atctttgcgg gaaatagcat gctgctcacg
     1801 cgggatcacc gtcacctgtg gacgggcgac tgcctgttca gcctcgcttt cctcgcggct
     1861 tagcaccttg cggcaaaaat tagcgactcg ggtaaaaata gtacacctcg gtagtgtcaa
     1921 acatcattca ggacaaaaaa atagcggcta atcatagctc agcatgacgc atttgagaat
     1981 gttgaattta caattgccga ctcgggcacg gcggtaagcc gccagttttt gacggctgac
     2041 tgaaggattt gctcgacgct gaaatcctgc cagtgtgctt ctgcctgctg ccccagaaat
     2101 tgaagtgccg cgattagtac cgggcgtgga tcgcctttcg gcaacgcagg cgcatgattc
     2161 tgcttggaaa gtttagcgcc ttgtggatta agcgccagcg gcagatgaat gtaatctggc
     2221 actttccagc caaaaagctg gtacagcgag atttgcctta ctgttggttc aatcagatca
     2281 gccccacgca ctatttctgt aacgccctgg aaatgatcat caaccacaac agccaggttg
     2341 taggcgaaca acccatcacg gcgatgaatg ataaaatctt cccgtgccag tttttcgtcg
     2401 gcgtgaataa tgccgcgcag ctggtcagta aattgcgtga ccggatgctg ctggcggata
     2461 cgcactgcgg cgttgtctgg tccatgatgc aacacccggc aatgaccgtc gtaaataccg
     2521 ccaatgcttt gaatacgcgc acgcgtacag gtgcagtaat aacttagtcc ttgttcatgt
     2581 aaccaggcga gtgcttcacg ataggcgtcg tgacgttgcg attgccagag aacatcgccg
     2641 tcccagtgca gaccgtaatg ttccagctgg cgcaggatag tttctgcggc accgggaact
     2701 tcacgaggcg ggtcgatatc ttctatgcgt accagccagc gaccttgccg ggcgcgagcc
     2761 tgcaaatagc tgccgagcgc ggcgatcaga gagccaaaat gaagctcgcc ggaaggagag
     2821 ggggcgaagc ggccaatata ctgtgtgtct gtcatctctt tgaacaaaaa ataaggcggg
     2881 agcatttccc gcctgtggta aacgtgatgg aacggctgta attagccagc catctgtttt
     2941 tcgcgaattt cagccagcgt tttgcagtcg atgcacagat cggctgtcgg gcgcgcttcc
     3001 agacggcgaa taccaatttc aacaccgcag gattcgcagt agccgaaatc ttcgtcttcc
     3061 acttttttca gcgtcttctc gatctttttg atcagcttac gctcgcgatc gcggttacgc
     3121 agttcgaggc tgaactcttc ttcctgggct gcacggtcta ccgggtccgg gaagttggct
     3181 gcttcatcct gcatatgtgt aacggtgcga tcgacttcat ccctgagttg attacgccat
     3241 gcttccagaa tacgacggaa gtgcgccagc tgggcttcat tcatatactc ttcgcccggc
     3301 ttctcctgat atggttccac cccagcgatg gcgagaatac tcagggacga tgttttacgg
     3361 ttttgccctt cttgcatgtt gcttctcctt aacacgcact atcgatcccc atgttcgggg
     3421 gaaaaatgag gccgctataa atagcagatg cttttccgga tagcaattat ctaaacgtaa
     3481 cacttgacaa ctgtgtgagg aaaagcgtat ttgcgcacgc gaccagaatg taaattaacc
     3541 agttacttac tttactacaa tgtaaccggc agtgattttt taagagccat gccttcagca
     3601 gaaatttccg ctttgtaagc cagaatttct acccccctct gttgagcttc tgacaatagt
     3661 tgcgcgtatt tctcatcgat gtggcgcgcg ggtgaaaacc gtgtaatggc tgaatgcagc
     3721 acggcgaaaa agataaccgc acgctggcct tcagccgcta cgctcatcaa ctcccgaagg
     3781 tgtttctgac ctcgttcagt gaccgcatcg ggaaaatatc cctgttcgtt ctccgctaac
     3841 gtaaccgatt tcacttcaat atagcagtct ggacgcgaat ccgcctgcaa cataaagtca
     3901 atacggctgc gttctgcgcc gtattttact tcgcttttca gcgagctata gcctgacagt
     3961 tctgaaattg attcattaag gatagcctct ttcgtcaacc tgttagccca aagcgtattg
     4021 acgcaaataa atgcgccgct ctggctttga gttagttccc aggtgtgtgg gtatttccgt
     4081 ttggtgttgt ctgaagtcga ataccagacg gtatcgccag gcgttgcaca accggtcatc
     4141 gcacccgtat tcgggcagtg tagcgttaat tcgcgaccat cgggtgtgat cacatcggct
     4201 aaaaaacgtt tgtaacgctg aattagcgtc gcgcgctgta gagggggaga aaattccatt
     4261 gcgacaatcc ttgttattgc gttagcgccc agcgttttag cggcgtgtag cgtgtgcgtc
     4321 cacgggcaaa cgaggaggcg taaagggtga actccgtcac cgcataagac cagttaaaac
     4381 ctggcggcgg gattgtcacc gcctcgctgg cgtcgcgcaa taaggtaata tgtggatgaa
     4441 acggacgatt gctttgaaaa caaccgctgc gggcagcctg tgaacggagc atattcgcca
     4501 gctggattaa gccgcgtgga ggctgacgca tccctaacca caccacacgc gaacgcagcc
     4561 attgtccggc gtcatcaagc gtgagtgtga aaccaggttg acgaatccgt ccggctaaaa
     4621 gagaaagcgc cttctctttc tcggcgctca cttcgcctaa aaatgccaga gtcagatgca
     4681 aattatcggc ggcgaccgga cgtcccgcct caggtgggaa gtgtgtggcg cgccaatgga
     4741 taatctgttc gcggatttct gcaggtaagt cgatagcaaa gaacagacgt tgcggttcag
     4801 acatgtgagg cactcggtta tgaattaccg cgatgctaca atgtggcgcg aagaatgtta
     4861 accctctgga gcgttttgtg tcgtcgttgc ccgttgctgc cgtcttatct gaattactta
     4921 ccgctctcga ttgtgcgccg caggtattat taagtgcgcc gaccggggcc gggaaatcaa
     4981 cctggctgcc gctgcaactg ctggcgcatc ccggcattaa cgggaaaatt atcctgctgg
     5041 agccgcgtcg tctggcggcg cgtaacgtcg cgcaacgact ggcggagctg cttaacgaaa
     5101 agccaggcga taccgttggc taccggatgc gtgcgcaaaa ctgcgtcggg ccgaataccc
     5161 gcctggaagt ggttaccgaa ggcgtgctga cgcgcatgat ccagcgtgac ccggaactga
     5221 gcggtgttgg attggtgatc ctcgatgaat ttcatgagcg cagcttgcag gcggatttgg
     5281 cgttggcgct gttactcgat gtgcaacaag gtctgcgtga tgaccttaaa ctgctgatta
     5341 tgtcggctac gctggataac gaccgcttgc agcaaatgct gccagaagcg cccgtcgtca
     5401 tctcagaagg gcgctcgttt ccggttgaac gccattattt accgctgccc gcgcatcagc
     5461 gttttgacga agccgttgcg gtagccaccg ccgaaatgct gcgtcaggaa agcggatcat
     5521 tactgttatt tttacctggc gtcggagaaa ttcagcgtgt gcaggagcaa ctggcttcgc
     5581 gcatcggcag tgatgtgttg ctctgcccgc tgtatggcgc gttgtcgctg aacgatcagc
     5641 gaaaagcgat cctcccggca ccgcaaggga tgcgcaaagt ggtgctggcg accaatattg
     5701 ctgaaaccag tttaaccatt gaaggtattc gtctggtggt ggattgtgcc caggagcgtg
     5761 tggcgcgttt tgatccgcgc acggggctta cgcgactgat tactcaacgc gttagccagg
     5821 catccatgac gcagcgtgcc gggcgcgccg ggcgtctgga gccgggtatc agcctgcatt
     5881 taatcgccaa agaacaagca gaacgcgccg cggcgcaaag tgaaccggag atcttacaaa
     5941 gcgatctttc cggttttctg atggaattac tgcaatgggg atgcagcgat ccggcgcaga
     6001 tgagctggct ggatcaaccg ccaacggtga atctactggc cgcgaaacgc ctgttacaaa
     6061 tgctgggggc gctggagggt gaacggctta gtgcgcaggg gcaaaaaatg gcagcgctgg
     6121 gtaacgatcc gcgtttagcg gcaatgctgg ttagcgcgaa gaacgacgac gaagctgcta
     6181 ccgcggcaaa aattgccgcc attctcgaag agccgccacg gatgggcaat agtgacctgg
     6241 gcgtggcgtt ttcgcgcaat caaccagcct ggcagcaacg tagtcagcaa ctgttaaaac
     6301 gcttaaacgt acgtggcggt gaggcagaca gttcgcttat cgcgccgcta cttgccgggg
     6361 cgtttgccga tcgcattgct cgtcgccgtg ggcaagatgg acgctatcaa ctggcaaacg
     6421 gcatgggagc gatgctcgat gccaacgacg cgctaagccg ccacgaatgg ttgatcgcac
     6481 cgttattatt gcagggcagc gcctcgccgg atgcgcggat tttactggcg ttaccggtcg
     6541 atattgatga attagtacaa cgctgtccgc agctggtgca gcaatctgac accgtggaat
     6601 gggatgacgc gcaaggtacg ctgaaagcct ggcgtcggct gcaaatcggt cagttgacgg
     6661 tgaaagtgca gccactggcg aaaccgtcag aagacgagtt gcatcaggcg atgcttaacg
     6721 gcattcgtga aaaaggttta agcgtgctca actggacggc ggaagcggaa cagctacgct
     6781 tgcgtttgtt atgcgccgca aagtggttgc cggaatatga ctggccagcg gttgatgatg
     6841 aaagtttatt ggcgacgctg gaaacgtggc tgctgccaca tatgactggc gtacattcac
     6901 tacgcggcct gaaatcactc gacatttatc aggcactacg cggattactt gattggggaa
     6961 tgcagcaacg tctggatagt gaattgcctg cgcattacac tgtgccgacg ggaagccgga
     7021 tcgccattcg ttatcatgaa gataacccgc ccgcgctggc ggtgagaatg caagagatgt
     7081 ttggcgaggc caccaatccg acgatcgccc aggggcgcgt gccgctggtg ctggagttgc
     7141 tttcacctgc ccaaaggcca ttacaaatca cacgagattt gagcgccttc tggaaaggag
     7201 cgtaccgtga ggtgcaaaaa gagatgaaag ggcgttatcc caaacatgtc tggccggacg
     7261 acccggcaaa tactgcaccg acgcgacgga cgaaaaagta ttcgtaaggt tggttttctc
     7321 cctctccctg tgggagaggg ccggggtgag ggcatcagcg cgcacgttca ccctcaccct
     7381 aaccctctcc ctcaagggag aggggaccga tcgagcacaa attttgagag atatcttctt
     7441 ctgtcttgta acagaagaac agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa
     7501 gcatggccgg gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg cgtccggtca
     7561 aacaaaaggt aagccgtcgt cgttacgaag atgacgatga ttacgacgat tatgatgact
     7621 atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg cgtaagcctc
     7681 gtggcaaacg cggctggcta tggctactgc taaaactggc tatcgttttt gccgtgctga
     7741 tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat ggcaaggtct
     7801 ggcaactgcc tgcggcagtt tatggccgaa tggtcaatct tgagccagac atgaccatca
     7861 gcaagaacga gatggtgaag ctgctggagg cgacccagta tcgtcaggtg tcgaaaatga
     7921 cccgtcctgg cgaatttacc gtgcaggcca acagcattga gatgattcgc cgtccgtttg
     7981 atttcccgga cagtaaagaa ggacaggtgc gcgcgcgtct gacctttgat ggcgatcatc
     8041 tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt cttgatccgc
     8101 gtctgatcac catgatctct tcgccaaacg gtgagcagcg tctgtttgtg ccgcgcagtg
     8161 gtttcccgga tttgctggtg gatactttgc tggcgacaga agaccgtcat ttttacgagc
     8221 atgatggaat cagtctctac tcaatcggac gtgcggtgct ggcaaacctg accgccggac
     8281 gcacggtaca gggtgcgagt acgctgacgc aacagctggt gaaaaacctg ttcctctcca
     8341 gcgagcgttc ttactggcgt aaagcgaacg aagcttacat ggcgctgatc atggacgcgc
     8401 gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc ggtcagagcg
     8461 gcgacaacga aatccgcggc ttcccgctgg caagcttgta ttactttggt cgcccggtag
     8521 aagagctaag cctcgaccag caggcgctgt tagtcggtat ggtgaaaggg gcgtccatct
     8581 acaacccgtg gcgtaaccca aaactggcgc tggagcgacg taatctggtg ctgcgtctgc
     8641 tgcaacagca acagattatt gatcaagaac tctatgacat gttgagtgca cgtccgctgg
     8701 gtgttcagcc acgcggtggg gtgatctctc cacagccagc ctttatgcaa ctggtgcgtc
     8761 aggagctgca ggcgaaatta ggcgataagg taaaagatct ctccggcgtg aagatcttca
     8821 ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa ggcattccgg
     8881 cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc gattgtggtc gtcgaccgct
     8941 ttagtggtga agttcgtgcg atggtcggtg gttctgagcc gcagtttgcg ggctacaacc
     9001 gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact tatctgactg
     9061 ccttaagcca gccgaaaatc tatcgtctga atacgtggat tgcggatgcg ccaatcgcgc
     9121 tgcgtcagcc gaatggccag gtctggtcac cgcagaatga tgaccgtcgt tatagcgaaa
     9181 gcggcagagt gatgctggtg gatgcgttga cccgttcgat gaacgtgccg acggtaaatc
     9241 tggggatggc gctggggctg cctgcggtta cggagacctg gatcaaactg ggcgtaccga
     9301 aagatcagtt gcatccggtt ccggcgatgc tgctgggggc gttgaactta acgccaatcg
     9361 aagtggcgca ggcgttccag accatcgcca gcggcggtaa ccgtgcaccg ctttctgcac
     9421 tgcgttcggt aattgcggaa gatggcaagg tgctgtatca gagcttcccg caggcggaac
     9481 gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag gtggtacaac
     9541 gcggtacggg tcgtcagctt ggggcgaaat acccgaacct gcatctggca gggaaaacag
     9601 ggactaccaa caataacgta gatacctggt ttgcaggcat tgacggcagc acggtgacca
     9661 tcacctgggt aggccgtgat aacaaccagc cgaccaaact gtatggagcc agcggggcaa
     9721 tgtcgattta tcagcgttat ctggctaacc agacgccaac gccgctgaat cttgttccgc
     9781 cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc agcggtggca
     9841 tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag agcgagatgc
     9901 agcagcagcc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa cagccgcaac
     9961 agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg atcaaggata
    10021 tgtttggtag taattaacat ctaagcgtga aataccggat ggcgagttgc catccggtaa
    10081 aataacatcc catctaagat attaaccctt tcttttcatc tggttgttta ttaacccttc
    10141 aggaacgctc agattgcgta ccgcttgcga acccgccagc gtttcgaata ttatcttatc
    10201 tttataataa tcattctcgt ttacgttatc attcacttta catcagagat ataccaatgg
    10261 cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt gtagtagcca
    10321 cagcggtaag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa gaagacacta
    10381 tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct gcaactattg
    10441 cggcgcgaca gtctgctacc ggcactaaaa ccgatacgcc gattcaaaaa gtgccacagt
    10501 ctatttctgt tgtgaccgcc gaagagatgg cgctgcatca gccgaagtcg gtaaaagaag
    10561 cgcttagcta cacgccgggt gtctctgttg gtacgcgtgg cgcatccaac acctatgacc
    10621 acctgatcat tcgcggcttt gcggcagaag gccaaagcca gaataactat ctgaatggcc
    10681 tgaagttgca gggcaacttc tataacgatg cggtcattga cccgtatatg ctggaacgcg
    10741 ctgaaattat gcgtggcccg gtttccgtgc tttacggtaa aagcagtcct ggcggcctgt
    10801 tgaatatggt cagcaagcgt ccgaccaccg aaccgctgaa agaagttcag tttaaagccg
    10861 gtactgacag cctgttccag actggttttg actttagcga ttcgttggat gatgacggtg
    10921 tttactctta tcgcctgacc ggtcttgcgc gttctgccaa tgcccagcag aaagggtcag
    10981 aagagcagcg ttatgctatt gcaccggcgt tcacctggcg tccggatgat aaaaccaatt
    11041 tcaccttcct ttcttacttc cagaacgagc cggaaaccgg ttattacggc tggttgccga
    11101 aagagggaac cgttgagccg ctgccgaacg gtaagcgtct gccgacagac tttaatgaag
    11161 gggcgaagaa caacacctat tctcgtaatg agaagatggt cggctacagc ttcgatcacg
    11221 aatttaacga cacctttact gtgcgtcaga acctgcgctt tgctgaaaac aaaacctcgc
    11281 aaaacagcgt ttatggttac ggcgtctgct ccgatccggc gaatgcttac agcaaacagt
    11341 gtgcggcatt agcgccagcg gataaaggcc attatctggc acgtaaatac gtcgttgatg
    11401 atgagaagct gcaaaacttc tccgttgata cccagttgca gagcaagttt gccactggcg
    11461 atatcgacca caccctgctg accggtgtcg actttatgcg tatgcgtaat gacatcaacg
    11521 cctggtttgg ttacgacgac tctgtgccac tgctcaatct gtacaatccg gtgaataccg
    11581 atttcgactt caatgccaaa gatccggcaa actccggccc ttaccgcatt ctgaataagc
    11641 agaaacaaac gggcgtttat gttcaggatc aggcgcagtg ggataaagtg ctggtcaccc
    11701 tgggcggtcg ttatgactgg gcagatcaag aatctcttaa ccgcgttgct gggacgaccg
    11761 ataaacgtga tgacaaacag tttacctggc gtggtggtgt taactacctg tttgataatg
    11821 gtgtaacacc ttacttcagc tatagcgaat cgtttgaacc ttcttcgcaa gttgggaagg
    11881 atggtaatat tttcgcaccg tctaaaggta agcagtatga agtcggcgtg aaatatgtac
    11941 cggaagatcg tccgattgta gttactggtg ccgtgtataa tctcactaaa accaacaacc
    12001 tgatggcgga ccctgagggt tccttcttct cggttgaagg tggcgagatc cgcgcacgtg
    12061 gcgtagaaat cgaagcgaaa gcggcgctgt cggcgagtgt taacgtagtc ggttcttata
    12121 cttacaccga tgcggaatac accaccgata ctacctataa aggcaatacg cctgcacagg
    12181 tgccaaaaca catggcttcg ctgtgggctg actatacctt ctttgacggt ccgctttcag
    12241 gtctgacgct gggcaccggt ggtcgttata ctggctccag ctatggtgat ccggctaact
    12301 cctttaaagt gggaagttat acggtcgtgg atgcgttagt gcgttatgat ctggcgcgag
    12361 tcggcatggc gggctccaac gtggcgctgc atgttaacaa cctgttcgat cgtgaatacg
    12421 tcgccagctg ctttaacact tatggctgct tctggggcgc agaacgtcag gtcgttgcaa
    12481 ccgcaacctt ccgtttctaa tttctctttt ggggcacgga tttccgtgcc catttcacaa
    12541 gttggctgtt atgcaggaat acacgaatca ttccgatacc acttttgcac tgcgtaatat
    12601 ctcctttcgt gtgcccgggc gcacgctttt gcatccgctg tcgttaacct ttcctgccgg
    12661 gaaagtgacc ggtctgattg gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct
    12721 tggccgtcat cagcctccgt cagaagggga gattcttctt gatgcccagc cgctggaaag
    12781 ctggagcagc aaagcgtttg cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc
    12841 agaagggatg accgtgcgtg aactggtggc gattggtcgt tacccgtggc atggcgcgct
    12901 ggggcgcttt ggtgcggcag atcgcgaaaa ggtcgaggaa gctatctcgc tggttggctt
    12961 aaaaccgctg gcgcatcggc tggtcgatag tctctctggc ggcgaacgtc agcgggcgtg
    13021 gatcgccatg ctggtggcgc aggatagccg ttgtctgttg ctcgacgaac cgacctcggc
    13081 gctggatatc gcccaccagg ttgatgtgct gtcgctggtg caccgtttaa gtcaggagcg
    13141 tggcctgacg gtcattgccg tgttgcacga tatcaatatg gcggcacgct actgtgatta
    13201 tctggtcgcc ctgcgcggcg gtgaaatgat tgttcaggga acgcctgcgg aaattatgcg
    13261 cggcgaaacc ctcgaaatga tttatggcat cccgatgggt attttgccgc atccggcggg
    13321 tgctgcacct gtgagttttg tttattgatg agcggcttac ctcttatttc gcgccgtcga
    13381 ctgttaacgg cgatggcgct ttctccgttg ttatggcaga tgaataccgc ccacgcggcg
    13441 gctattgatc ccaatcgtat tgtggcgctg gagtggttgc cggtggaatt actgctggcg
    13501 ctcggcatcg tgccttacgg cgtggcggat accatcaact atcgcctgtg ggtcagcgaa
    13561 ccaccattgc cggactcagt gatcgacgtc ggtttgcgca cagaacctaa ccttgaactg
    13621 ctgaccgaaa tgaaaccatc gtttatggtc tggtcggcag gatatggccc ttcaccagaa
    13681 atgctggctc gtattgcgcc ggggcgtgga tttaacttca gcgacggcaa acagccgctg
    13741 gcgatggcgc gtaagtcact gacggaaatg gcagatttac ttaacctgca aagcgcagca
    13801 gaaacgcatt tagcgcacta tgaagacttt atccgcagca tgaaaccccg ctttgtgaag
    13861 cgtggtgaac gcccgttatt gctgacgacg cttatcgatc cgcgccatat gctggttttc
    13921 ggtccaaaca gcttgtttca ggaaattctt gatgagtacg gcatcccaaa tgcctggcaa
    13981 ggggaaacca acttctgggg cagtaccgcc gtcagcatcg atcgtctggc ggcgtataaa
    14041 gatgttgatg tgctctgttt tgatcacgac aacagcaaag acatggacgc gctaatggca
    14101 accccgctgt ggcaggccat gccgtttgtc cgcgccggac gctttcagcg cgtacccgca
    14161 gtctggtttt atggtgcgac gctctcggca atgcactttg tgcgcgttct ggataacgcc
    14221 atcggaggta aagcgtgagt aaacgaattg cgctttttcc ggcgttattg ctggcgctgc
    14281 tggtgattgt cgctacggcg ctgacctgga tgaacttttc gcaggcgctg ccgcgtagcc
    14341 agtgggcgca ggccgcctgg tcgccggata ttaacgtcat cgagcagatg atttttcact
    14401 acagcttgtt accacgtctg gcgatttctc tactggtggg cgcgggcctg gggctggtgg
    14461 gcgtgctgtt tcagcaagtg ctgcgtaacc cgctggcgga accgacgacg ttgggcgttg
    14521 ctacaggcgc gcaactgggg attaccgtca ccacgctctg ggcgatccca ggtgctatgg
    14581 cgagccagtt tgctgcgctg gcaggggctt gcgttgttgg cttaattgtc tttggcgtcg
    14641 cgtgggggaa acggctgtcg ccggtaacgc tgattctcgc ggggctggta gtgagccttt
    14701 attgtggggc aatcaatcag ttactggtta tcttccatca tgaccagttg caaagcatgt
    14761 tcctgtggag caccggaacg ctgacgcaaa ccgactgggg cggcgttgag cgtttatggc
    14821 cgcagttgct gggcggcgtg atgctgacgt tactgctact tcgtccgtta accctgatgg
    14881 ggcttgatga tggcgtggcg cgcaatctcg ggctggcctt gtcgctcgcg cgtctggcgg
    14941 cgctgtcgct ggcgatagtc atcagtgcgc tgctggtgaa cgcagtgggg attatcggct
    15001 ttatcgggtt gttcgcgcca ctgctggcaa aaatgctggg ggcgcggcgt ttgttgccac
    15061 ggttgatgct ggcgtcgctg attggtgcgt taattctgtg gctttctgat caaatcatcc
    15121 tctggctgac tcgcgtgtgg atggaagttt ccaccggttc ggtcactgcg ttgatcggtg
    15181 cgccgctgct actgtggctg ctgccgcgtt tacgcagcat tagcgcgccg gatatgaagg
    15241 ttaacgatcg tgtcgcgact gaacgccaac atgtgctggc gtttgccctc gcgggcggcg
    15301 tgctgctgtt gatggctgtg gtggtggcgc tgtcgtttgg tcgtgatgcg cacggctgga
    15361 cgtgggcgag cggggcgctg ctcgatgatt taatgccctg gcgctggccg cgaattatgg
    15421 cggcgctgtt tgcgggcgtc atgctggcgg tagcgggctg tattattcag cggctgaccg
    15481 gcaacccgat ggcaagcccg gaagtgctgg ggattagctc cggcgcggcg tttggtgtgg
    15541 tgttaatgct gtttctggta ccgggtaatg cctttggctg gctgttaccc gcaggcagtc
    15601 tcggggcggc ggtgacgctg ttgatcatta tgatcgccgc cggacgcggt ggattttccc
    15661 cacatcgcat gttactggcg gggatggcgt taagtaccgc gttcaccatg cttttgatga
    15721 tgttgcaggc aagtggtgac ccgcgaatgg cgcaagtgct gacctggatt tccggttcga
    15781 cctacaacgc gaccgatgcg caggtctggc gcaccggaat tgtgatggtg attttgctgg
    15841 cgattacccc gctgtgccgc cgctggctga ccattttacc gctgggtggt gataccgccc
    15901 gagccgtagg aatggcgctg acgccgacgc gaattgcgct gctgctgtta gcggcttgcc
    15961 tgacggcgac cgcgacgatg actattggac cgttgagttt tgttggttta atggcaccgc
    16021 atattgcgcg gatgatgggc tttcgacgga cgatgccaca catcgtaatt tcggcgctgg
    16081 tgggggggtt actgctggtg ttcgctgact ggtgtgggcg gatggtgctg tttccattcc
    16141 agatcccggc ggggctgctg tcaaccttta tcggcgcgcc atattttatc tatttgttga
    16201 gaaagcagag ccgttaattg aatgcctcgc ccggtgggcg aggcaaaatc atcacaactt
    16261 cgcaaacacc cgacgtgcag catcgatggt gttattgata tcttccatgc tgtgcgccac
    16321 ggacataaag cccgcttcaa acgctgacgg tgccaggtaa acaccttcat ccagcatcat
    16381 atggaagaaa cgcttaaagc gttccacgtc acaggccatc acatcctgat agcacgtcac
    16441 ggactcggcg tcggtaaaga aaataccgaa catgccgcca acgtggttaa cgaccagcgg
    16501 aattccggct tcttctgccg cttccagcag accttctgcc agacgtgatg tcagctcatc
    16561 caacgtttcg tgaacgcccg gttgcgcgac ttcattcaga caggcgaaac ccgctgccat
    16621 cgcaattggg ttaccggaaa gcgtacccgc ctgatagacc ggacccgtcg gggccagcgc
    16681 atccattaca tcacgacgac caccgaatgc gcctaccggc attccaccgc cgatgatttt
    16741 gcccaggcag gtgagatccg gttccacacc gtaataatcc tgtgcgccag ctagcgctac
    16801 gcggaagcca gtcattactt catcgatgat cagcaatgcg ccaaattcgt cgcacagcgc
    16861 gcgcagacct ggcaggaact ctggcagcgg tggaacgcag ttcatattgc ctgccaccgg
    16921 ctcgacgata atacaggcaa tctcttgcgg gtattgctca aacgcggcgc gtacagaagc
    16981 cagatcgtta taagtacagg ttaaggtatg tttggcgaaa tctgccggaa cacccggcga
    17041 gtttggctgg cctaacgtga gtgcgccaga accggctttc accagcaggc agtcagcgtg
    17101 accgtggtaa caaccttcaa atttaatgat tttgtcgcga ccggtaaaac cacgggccag
    17161 gcggatggcg ctcatggtcg cttcagtgcc ggagttcacc atgcgcacca tatccatggt
    17221 cgggaccagt tcggtcacca gttgcgccat tttcacttcc atttcggttg gtgcaccaaa
    17281 gcttaaacca cgctcggcgg cttcaatcac ggcattgcgg atcgccggat ggttatggcc
    17341 cagcaccatc ggcccccagg aaccgacata atcgatatag gctttgccat caacatcgta
    17401 cagataggcg ccgtccgctt tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc
    17461 gcgaacaggg gagttcacac cgccagggat cagctcgcgc gctgcgctgt aaagattttc
    17521 agacttactc atggagggtt cctgattcgt agaaaaagtg aatggctgct attctatgtt
    17581 attcataaca agttaaatac tcgtcaaaca tcaggctgct tgtactggtg caatcctgat
    17641 ttcttagagt ataaaagttt tgtgcatttg aaacattacg ctttgcaaag gattttcatg
    17701 gaacgtgcga gtaaaatgcc gtcatcttat ttgtatgacc aataagtgat cattggatga
    17761 aaactgatac tccctcttta gaaacaccgc aggccgcgcg cctgcgacgt agacaactga
    17821 ttcgccaact tcttgagcgc gataaaaccc cgttagccat tttgtttatg gcggcagtcg
    17881 tcggcacgct tgtcgggctg gcagcggttg cttttgacaa aggtgtcgcc tggttgcaga
    17941 accaacgtat gggggcgctg gtacatactg ctgataatta tccgctactg ttaaccgtcg
    18001 cttttctctg ttcggcggtg ctggcgatgt tcggctactt tttggtgcgc aaatacgcgc
    18061 ccgaagcagg gggatcgggg atcccggaaa ttgaaggggc gctggaagat caacgtcctg
    18121 ttcgctggtg gcgtgtattg ccggtgaaat tctttggcgg gctggggaca ctcggcggag
    18181 gcatggtgct agggcgcgaa ggaccaaccg tgcagatcgg cggcaatatc ggtcgcatgg
    18241 tgcttgatat tttccgcctg aaaggtgatg aagctcgcca tacgctgctg gcaaccggtg
    18301 ctgctgcggg gctggctgcg gcctttaacg cgccgctggc gggtattttg tttattatcg
    18361 aagagatgcg tccgcagttt cgctatacgt taatttcgat taaagcggta tttattggcg
    18421 tcattatgtc gaccattatg taccggattt ttaatcatga agttgcgttg attgacgtcg
    18481 gtaaactttc tgatgcgccg cttaatacgc tgtggcttta tctgatcctc ggtattattt
    18541 ttggcatttt cggccctatt tttaataaat gggtgctggg gatgcaggat ttgctgcacc
    18601 gtgtgcacgg cggcaatatt accaaatggg tactgatggg tggtgcgatt ggcggtctgt
    18661 gtggattgct ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac ctgattccta
    18721 tcgctaccgc ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc gctcgggtca
    18781 ttaccacctt actctgcttc tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg
    18841 cgctgggtac tgtgctggga accgctttcg gaatggttgc cgttgagctg tttccgcaat
    18901 atcacctgga ggcggggacg tttgctattg ccggaatggg ggcattactg gcggcatcta
    18961 ttcgcgcgcc gttaacgggg atcattctgg ttctggagat gaccgataac taccagctca
    19021 ttttgccaat gattattacc ggtcttggcg caacactatt agcgcaattt accggcggga
    19081 aaccgctata ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct gagcaactgg
    19141 cgcgaagcaa ggccgcatca gccagcgaga atacttgaac gaaataccag ggtattagat
    19201 aatggcgatt attattgggt tagaatttgc ccaattgccg atgtcgtttg gagcaaaata
    19261 tgagtgatga cgtagcactg ccgctggagt ttaccgacgc agcagccaac aaagttaaaa
    19321 gcctgatcgc tgacgaagat aacccgaatc tgaaattacg cgtgtatatc accggtggcg
    19381 gttgcagcgg cttccagtat ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga
    19441 ccatcgaaaa acagggcgtt ggcctggtgg ttgatccgat gagcctgcaa tatctggtcg
    19501 gcggttccgt tgattatacc gaaggtctgg aaggttctcg tttcatcgtg accaacccga
    19561 acgcgaaaag cacctgcggt tgcggttctt cctttagcat ctaatctgtt gttcatgtgc
    19621 cggataaact atccggcaca tcttctctct tcaacgccca ttctcatcca gcgcaaacgt
    19681 cggtagctta agatgccaac gaatagccgc cagccgaatc aatagcgtca cgaccatgcc
    19741 catcatactg gctgtttcca gtggtacgga aaatgtgtaa taagccgtag cgtggacaat
    19801 accgccgata atacaggcag ttgcgtagat ttctgtacgt aaaatcatgg ggatctcgcg
    19861 ggccagtaca tcacgaatga tcccgccgcc aacgccagta atgacgccca tgcaaaccgc
    19921 gattaacgga ccggcttccg cattaaaggc tttattcacg ccaatgccga caaacaccgc
    19981 cagaccaacg gcgtccagca ccggcaacat ccattttggt aagcgtcttg gctggcgcac
    20041 cagcacgatg gtcagcatgc tggtgaccat tgcaacgacc agatcggtgg gatctttcac
    20101 ccaaaatacc gggccgtgat ccagcgccat gtcgcgaatt gtcccgccgc ctactgcggt
    20161 taccacgccc agtaccagaa caccaaaagg gtccatacgc aattttccgg ctaacaaaac
    20221 gccggagatg gcaaataccg ctgtgccgac tatatccagc caatagacga gcattgttca
    20281 atccccactg agcacctgaa aaggtcaggc gctaatctac ctgtgaaagc gcattacaga
    20341 gctgttgtgc agcgaggata atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag
    20401 gaataacggg aattttgagc tgttcacccc agtattgttt gattttagga atttggtccg
    20461 gtccgcctgt aatgacaatc gcctgtggcg agcgtgctaa cacctgttcg cggctaactt
    20521 gcggccaggg aacccggctg tctttaaaga tgttttctcc gccacaaact tcgagtacct
    20581 ggttctgaat cgactctttt ccactggtaa atggcggatt aatgccgaat tgcagaaaaa
    20641 cacgtttttt aggtttatca gcatattgcg ctttcaattg cgcgtactga tccagcaggg
    20701 attgcgcggc ttgttcggcc ttgtctggtt gcggactcca gggggccagt tgacgtaacg
    20761 cattggcaat ttgttcaatg cttgtcgcat cgacccacat cacttttatt cccagcgaag
    20821 ccagctggtc aacctgccgc tcggcattac ctccacgcca ggcaatcacc agatcgggct
    20881 tcagcgcgac aatgcgttcc agattcatcc cctgccaggt ggaaacctgc tcaatctttt
    20941 gcgcttgtgg aggatagtcg gaatagctgc tgaccccaac cggcgtgatc ccggcagcaa
    21001 aggcaagttc agtgttggcg ggagaaagcg tgatgacgcg cggcgcggcg ttgagccaca
    21061 gtggcgcaag aaaagacagg gcgaccagcg ccctgaacag tgacttagcc atgtgcaagt
    21121 ttctgcacca gtgactcaac catcaggctg gactgtttag cggcaacagc caggaactca
    21181 tcgaagctaa gatgagactg ttgatcggcc acgtcggaga tggcgcgtac gacaacaaac
    21241 gggacgttga aattgtggca gacatgggcg attgccgtcg cttccatctc tacagcaatg
    21301 gcctgtggga agttgtggcg gattttcgcc agaccaacag aaccgttgat gaaagcgtcg
    21361 ccgctaacaa tcaggccacg tacagcgtta agattcagtt cggcaatgca ggcctcagcg
    21421 gcagcgatca gtttatcgtc agctttaaag cctgccggac agcctggtaa ctgaccgtat
    21481 tcataaccaa atgccgtgac atccgcgtcg tgataacgtg cttcgtccga gacaacgata
    21541 tcgcccactt tcaacgttgg tgccaggcca ccggcagaac cggtgttaat aatcacatct
    21601 ggcttgcagt gttccaacag cagagtggca cccagcgccg cagcgacttt accgatgccc
    21661 gatttcagaa gcgcaacctc ggttccattc agttggccgg tatagatttc gcaaccgccg
    21721 agactgatag tttgacggtt ttcgattttg tcacgcagca gcgtaacttc ttcttccatt
    21781 gcaccaatga tgccgatttt catagattta ctcgcgataa gcccgatttg aaggcatagt
    21841 ttaccatgcg cttacgggga agcgtatttc tcacgcggga gaggacatgg cacagattga
    21901 tttccgaaaa aaaataaact ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga
    21961 acatgagatc ctgcggatct tcgagagcga tcgcgggcgt atcatcaact ctccggcaat
    22021 tcgtcgtctg caacaaaaga cccaggtttt tccactggag cgcaatgccg ccgtgcgcac
    22081 gcgtcttacc cactcgatgg aagtccagca ggtggggcgc tacatcgcca aagaaatttt
    22141 aagccgtctg aaagagctta aattgctgga agcatacggc ctggatgaac tgaccggacc
    22201 ttttgaaagc attgttgaga tgtcatgcct gatgcacgat atcggcaatc cgccgtttgg
    22261 tcattttggc gaagcggcga taaatgactg gtttcgccag cgtttatacc cggaagatgc
    22321 cgaaagccag cctctgactg acgatcgctg cagcgtggcg gcactacgtt tacgggacgg
    22381 ggaagaaccg cttaacgagt tgcggcgcaa gattcgtcag gacttatgtc attttgaggg
    22441 gaatgcacaa ggcattcgtc tggtgcatac attgatgagg atgaatctca cctgggcaca
    22501 ggttggcggt attttaaaat atacccgtcc ggcgtggtgg cgtggcgaaa cgcctgagac
    22561 acatcactat ttaatgaaaa agccgggtta ttatctttct gaagaagcct atattgcccg
    22621 gttgcgtaaa gaacttaatt tggcgcttta cagtcgtttt ccattaacgt ggattatgga
    22681 agctgccgac gacatctcct attgtgtggc agaccttgaa gatgcggtag agaaaagaat
    22741 atttaccgtt gagcagcttt atcatcattt gcacgaggcg tggggtcagc atgagaaagg
    22801 ttcgctcttt tcgctagtgg ttgaaaatgc ctgggaaaaa tcacgctcaa atagtttaag
    22861 ccgcagtacg gaagatcagt tttttatgta tttacgggta aacaccctaa ataaactggt
    22921 accatacgcg gcacaacgat ttattgataa tctgcctgcg attttcgccg gaacgtttaa
    22981 tcatgcatta ttggaagatg ccagcgaatg cagcgatctt cttaagctat ataaaaatgt
    23041 cgctgtaaaa catgtgttta gccatccgga tgtcgagcag cttgaattgc agggctatcg
    23101 ggtcattagc ggattattag agatttatcg tcctttatta agcctgtcgt tatcagactt
    23161 tactgaactg gtagaaaaag aacgggtgaa acgtttccct attgaatcgc gcttattcca
    23221 caaactctcg acgcgccatc ggctggccta tgtcgaggct gtcagtaaat taccgtcaga
    23281 ttctcctgag tttccgctat gggaatatta ttaccgttgc cgcctgctgc aggattatat
    23341 cagcggtatg accgacctct atgcgtggga tgaataccga cgtctgatgg ccgtagaaca
    23401 ataaccaggc ttttgtaaag acgaacaata aatttttacc ttttgcagaa actttagttc
    23461 ggaacttcgc gctataaaac gaatctgaag aacacagcaa ttttgcgtta tctgttaatc
    23521 gagactgaaa tacatgaaaa aaaccacatt agcactgagt gcactggctc tgagtttagg
    23581 tttggcgtta tctccgctct ctgcaacggc ggctgagact tcttcagcaa cgacagccca
    23641 gcagatgcca agccttgcac cgatgctcga aaaggtgatg ccttcagtgg tcagcattaa
    23701 cgtagaaggt agcacaaccg ttaatacgcc gcgtatgccg cgtaatttcc agcagttctt
    23761 cggtgatgat tctccgttct gccaggaagg ttcaccgttc cagagctctc cgttctgcca
    23821 ggggggccag ggcggtaatg gcggcggcca gcaacagaaa ttcatggcgc tgggttccgg
    23881 cgtcatcatt gatgccgata aaggctatgt cgtcaccaac aaccacgttg ttgataacgc
    23941 gacggtgatt aaagtccagc tgagcgatgg tcgtaagttc gacgcgaaga tggttggcaa
    24001 agatccgcgc tctgatatcg cgctgattca gatccagaac ccgaaaaacc tgaccgcaat
    24061 taagatggcg gattctgatg cactgcgcgt gggtgattac accgtagcga ttggtaaccc
    24121 gtttggcctg ggagagacgg taacttccgg gattgtctct gcgctggggc gtagcggcct
    24181 gaatgccgaa aactacgaaa acttcatcca gaccgatgca gcgatcaacc gtggtaactc
    24241 cggtggtgcg ctggttaacc tgaacggcga actgatcggt atcaacaccg cgatcctcgc
    24301 accggacggc ggcaacatcg gtatcggttt tgctatcccg agcaacatgg tgaaaaacct
    24361 gacctcgcag atggtggaat acggccaggt gaaacgcggt gagctgggta ttatggggac
    24421 tgagctgaat tccgaactgg cgaaagcgat gaaagttgac gcccagcgcg gtgctttcgt
    24481 aagccaggtt ctgcctaatt cctccgctgc aaaagcgggc attaaagcgg gtgatgtgat
    24541 cacctcactg aacggtaagc caatcagcag ctttgccgca ctgcgtgctc aggtgggtac
    24601 tatgccggtg ggcagcaaac tgaccctggg cttgctgcgc gacggtaagc aggtcaacgt
    24661 gaatctggaa ctgcagcaga gcagccagaa tcaggttgat tccagctcca tcttcaacgg
    24721 cattgaaggt gctgagatga gcaacaaagg caaagatcag ggcgtggtag tgaacaacgt
    24781 gaaaacgggc actccggctg cgcagatcgg cctgaagaaa ggtgatgtga ttattggcgc
    24841 gaaccagcag gcagtgaaaa acatcgctga actgcgtaaa gttctcgaca gcaaaccgtc
    24901 tgtgctggca ctgaacattc agcgcggcga cagctccatc tacctgttaa tgcagtaatc
    24961 tccctcaacc ccttcctgaa aacgggaagg ggttctcctt acaatctgtg aacttcacca
    25021 caactccata catcttcatc atcctttagg catttgcaca atgccgtacg ttacgtactt
    25081 ccttatgcta agccgtgcat aacggaggac ttatggctgg ctggcatctt gataccaaaa
    25141 tggcgcagga tatcgtggca cgtaccatgc gcatcatcga taccaatatc aacgtaatgg
    25201 atgcccgtgg gcgaattatc ggcagcggcg atcgtgagcg tattggtgaa ttgcacgaag
    25261 gtgcattgct ggtactttca cagggacgag tcgtcgatat cgatgacgcg gtggcacgtc
    25321 atctgcacgg tgtgcggcaa gggattaatc taccgttacg gctggaaggt gaaattgtcg
    25381 gcgtaattgg cctgacaggt gaaccagaga atctgcgtaa atatggcgaa ctggtctgca
    25441 tgacggctga aatgatgctg gaacagtcgc ggttgatgca cttgttggct caggatagcc
    25501 gtttgcggga ggaactggtg atgaacctga ttcaggcaga ggagaatact cccgcactta
    25561 ctgaatgggc gcaacggctg gggatcgatc tcaatcaacc gcgagtggtg gctattgttg
    25621 aggtcgacag cggtcagctt ggtgtggaca gcgcaatggc ggagttacaa caactgcaaa
    25681 acgcgctgac tacgcccgag cgtaataatc tggtggcgat tgtctcgcta accgaaatgg
    25741 tggtgttgaa accggcgttg aactcttttg ggcgctggga tgcagaagat catcgtaagc
    25801 gagttgaaca actgattacc cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac
    25861 tgggcaacta ttttaccggt cctggcagta ttgcccgatc ctatcgtacg gcgaaaacga
    25921 cgatggtggt gggtaaacag cggatgccag aaagtcgctg ctatttttat caggatctga
    25981 tgttacctgt gttactcgac agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac
    26041 cgctggcgcg gctgaaagcg atggacaata acggcttgct gcgacgaacg ctggcggcgt
    26101 ggtttcgtca caatgtgcaa ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata
    26161 ctctggagta tcggcttaat cgtatatcgg aactgaccga gcttgatttg ggtaattttg
    26221 atgacaggtt gctgctgtat gtggcgttgc agctggatga agagcggtag gttatgcgtt
    26281 aaggtcgagg cggcgctggc tcattcccct cagcccgaag ggtagagggg aatgtccgag
    26341 ttaaccgtca gttcggcatt atttacgcgt tagtttttcc agatcggctt caatctcgct
    26401 gatcttattg gttacgactg actccaggtg acgtaagtcg tcgaggatct tacgcttaag
    26461 atcaacttcg ctgcggtcac gctggcagat ttgatcaagc tcatcaatga tataccgtag
    26521 attcgggctg atttcctgga cttctttata accctgaccc acaccatcag cgacgaccgt
    26581 tttacgctga cgcggatatt taaacttaac gctcttggcg aaaaactcgc ctttgtcttt
    26641 ctggaaatag attttcagaa tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat
    26701 ttcttcagga ttggtaatac ccagactttt cagattgtcg tacatagcgt tacctcaaaa
    26761 tgagtcagta aattgtgctt attttagcat ttggcctggc ccgcccggct cgactgtgat
    26821 cgcagaaagc ctttttcttc cagacttcta tcatggcgta aaaatcaaaa aattacctgc
    26881 tttattgtgg cgatattcca tgggatggat tagcacattt atcttcgttt tgcgaattac
    26941 atgacagtcc gaaacaagat gaaaaagtat ttaaaaaagg ggggaagaat gagtaggtcg
    27001 aattattctg agccagaaag catacgggca gcattatgct gcccgtatat gtactcttaa
    27061 tcgatggtac gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc
    27121 ttaacgataa ccgcacagta gaggctgtat ttgccatctt tcgacggcag attacctgaa
    27181 acaaccacag accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa
    27241 atacgggtgc tctgaccaat gtatacgccc atagagatga cggaaccttc ttcgacaatc
    27301 accccttcaa ccacttcgga gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg
    27361 ttagcctgca gcggctccag cacgccgccg atgcccacgc caccggaaag gtggacgttt
    27421 ttaccaatct gcgcacaaga accgacggtc gcccaggtat caaccatcgt gccttcatca
    27481 acatatgcgc cgatgttgac gtaagacggc atcagcacgg tgttacgggc aataaatgca
    27541 ccctgacgta ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg
    27601 tcgtagtcgg cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc
    27661 acctgattat cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc
    27721 acccactgac cgtcaatttt ttccgctaca cgcagtgcac cggaatccag cagggcgatc
    27781 acctgattta ccgcttcgcg ggtaacggtg tctgcgttgg ctggcgtgat ctcggcacgg
    27841 cgttcaaaag cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat
    27901 cagtaaacac atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact
    27961 tcctgctgca actcgttatt aagtgcacgc cggtcagcgg tggcaattat gaataaatct
    28021 tctactcgct cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca
    28081 aaaattttcc cgactctcgc cagcagtcct ggctggtcga gcgcgataag ctcaaggaac
    28141 gatttgcggt cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt
    28201 aatttggcgg gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagtacttgc
    28261 tccagaccaa accgaataac ctcatgacga tctgcggaca gcgggctgcc atcgggttcc
    28321 agcacgataa aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga
    28381 acacttaaat tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc
    28441 gggctccaga taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc
    28501 ggtttgctta aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta
    28561 tggcggacaa aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg
    28621 atgttatcca tgcgcagtag tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc
    28681 atatccggcg tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg
    28741 cgcaacagac tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc
    28801 actgtcaggc ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc
    28861 ttgatgactt cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc
    28921 tggcgaacca gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca
    28981 aaatgcacta catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata
    29041 tcgtgaaaca gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc
    29101 acacacaacg gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc
    29161 acgcggatag tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg
    29221 acgatatgcg accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat
    29281 agcccgcgcc gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttcc
    29341 ggaatattac acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg
    29401 gtggagtaaa tgccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg
    29461 gcttccggct ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc
    29521 tgaaactcat cgtcgattgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca
    29581 tcgaacagtt gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa
    29641 tccttcatca tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg
    29701 acgctaagtt ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg
    29761 gcaaagcgaa tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag
    29821 gttaagaagc caaacccaac catttcatcc agcgatgttg cgccaaaatg acggcgggcc
    29881 acccattgca gagtgtggat atcgcgcaag ccgccagggc tacttttgat gtctggttca
    29941 agattgtagc tggtgccgtg gtaacgctga tggcgctggt tctgttcttc aactttcgcc
    30001 gcgtagaact tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg
    30061 aagagcgcaa catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa
    30121 tccgataacc cttccagcat gcactcttca agcgtgcgca cactatgacc gacttccagc
    30181 tttacatccc agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc
    30241 tttttacggc ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg
    30301 tagccaccga cggcgaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat
    30361 aatcgttgca ggagctggtc gataaactcg gtgcgcgcct caatcaactg ttctgcagag
    30421 atcccattgt caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc
    30481 ccaccgacgg ttaattcatc acggggccag acgcatggat tttgcggttg accgggcagg
    30541 gtggggagag cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa
    30601 gattaaatta tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga
    30661 gattatcgcc gggatggtgt catccttgcg tagcgtcaaa atttcgcagc cgttatcagt
    30721 caccacaata gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca
    30781 gccatctttc atggtgcgga tctctttttt acccgcgttg accattggct caatggtgaa
    30841 cgtcatcccc ggtttcagca cgacgttggt ttcacgggag tcatagtgca gcacctgcgg
    30901 ttcttcatgg aagccgcgac caataccgtg tccacaatat tcacgaacga cggagaagcc
    30961 ttctgcttcg acaaacttct gaatcgccgc accgatttcg cgcaggttaa tgcctggttt
    31021 gaccatgcgc agcgccaggt acaggctttc ttgcgtgatg cggcacagac gttcgcccat
    31081 gatggtcggc ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat
    31141 tacggtgaca tcaatgttaa cgatatcgcc atctttcagc agcttagcat cgtccgggat
    31201 accgtggcac accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc
    31261 gaggcaggca gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag
    31321 ctcgccggtg ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc
    31381 cagtcggcca gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat
    31441 taattctgtc catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta
    31501 tcacacccgg gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt
    31561 ctataataga aaattcgaca tctgatgctg tacacagcgc caacaattat tggtgtccat
    31621 gacgtatttg tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga
    31681 cggaagcgac aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg
    31741 ccctttgggg tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt
    31801 tttaatcatg gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca
    31861 ccagacccgt tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt
    31921 tcacatcatc aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa
    31981 caagattgct tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga
    32041 agcggtgaaa gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg
    32101 cggtatgctg actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga
    32161 aactcagtct caggacggta ctttcgaaaa gctgaccaag aaagaagcgc tgatgcgcac
    32221 tcgtgagctg gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc
    32281 ggacgctctg tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa
    32341 cctgggtatt ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt
    32401 cgttatcccg ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc
    32461 tgcaaccgta cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt
    32521 agaagctgag taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg
    32581 caagctcgag aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagat
    32641 aacgcatctg cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt
    32701 ttggttaggg ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc
    32761 cgggcagatc acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa
    32821 gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct
    32881 aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg
    32941 aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac
    33001 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag
    33061 gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt
    33121 ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc
    33181 aacattcgcc gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg
    33241 cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct
    33301 atgcacgttg ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg
    33361 gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa
    33421 atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc
    33481 ggtcagccgt tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac
    33541 gctgaagtga ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact
    33601 gactttgcag cagaagttgc tgcgatgtcc aagcagtctt aattatcgaa aaggagccgc
    33661 ctgagggcgg cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg
    33721 ctgaaaagcg acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg
    33781 aaagaaacat ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg
    33841 gcgaagctct gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg
    33901 ctcaggaaat caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg
    33961 gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc
    34021 acatggggat gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg
    34081 cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca
    34141 gctgggcaga agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta
    34201 caggtaaccc gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag
    34261 ccgatgtggt gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag
    34321 atccaaccgc aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga
    34381 aagtcatgga cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt
    34441 tcaacatgaa caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt
    34501 taatcacgga ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg
    34561 ggggcgttag tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg
    34621 cagcaaccgg agcgtacaaa agtacgtgag gatggcgagc actgcccggg gccaaaatgg
    34681 caaataaaat agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac
    34741 ttagcacact tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc
    34801 gtaacgtgat tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag
    34861 cgttcaaaac ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg
    34921 gcattgtcgt ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg
    34981 tagaagattc ccgtacactg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg
    35041 aaaaagcgat tatggcgtcc gatcttggcc tgaacccgaa ctctgcgggt agcgacatcc
    35101 gtgttccgct gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg
    35161 gtgaagcaga acaagcgcgt gttgcagtac gtaacgtgcg tcgtgacgcg aacgacaaag
    35221 tgaaagcact gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg
    35281 atgtacagaa actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag
    35341 aagcagaact gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt
    35401 gcggatcggc tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag
    35461 caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc
    35521 cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg
    35581 gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg
    35641 aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt aagtgggcaa
    35701 caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc agccattgtt
    35761 ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg
    35821 gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc
    35881 aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag tttaccgcaa
    35941 cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta
    36001 cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg
    36061 ccggatcaag cctgccgtca tccgaactgg tcgatggggc gtaaaatttc tgtcgattcg
    36121 gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc
    36181 gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat
    36241 caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac
    36301 accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta
    36361 agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa actggcgatg
    36421 gaggcgttcg aacaaggtca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc
    36481 gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatcg
    36541 gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat
    36601 gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc
    36661 ggctacagag agtcgcgcta tttgttagcg tagggcttca gtgatatagt ctgcgccatc
    36721 tgatcgtaag tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt
    36781 tgtataggct tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga
    36841 tgttgtctgc tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg
    36901 cgatcattat ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg
    36961 ggcataaagc cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta
    37021 ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag
    37081 tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc
    37141 gacataacgt gcgtctgcgt attattggcg ataccagtcg ctttaactcg cgtttgcaag
    37201 aacgtattcg taaatctgaa gcgctaacag ccgggaatac cggtctgacg ctgaatattg
    37261 cggcgaacta cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg
    37321 tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga gatgctaaac cagcatgtct
    37381 gtatgcatga actggcccct gtagatttag taattaggac tgggggggag catcgcatta
    37441 gtaacttttt gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc
    37501 ccgatttcga tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc
    37561 gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt
    37621 atcgcctgat atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc
    37681 cgccggtggg gttcgccatc gtaacgctgg tggtctgcat gctggcagcg tgggaatggg
    37741 gacagcttag cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt
    37801 tattgttggc gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc
    37861 tggttgaaat ctcactttgg gcttcgctgg gttggtggat tgtcgcgcta ttgctggtgc
    37921 tgttttaccc aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg
    37981 gcgtgctaac cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactacg
    38041 acgagaatca ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggggcg
    38101 ctgactctgg cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg
    38161 tttctccggg taaaacctgg caaggcttta taggtggact agcgaccgca gcggtaatct
    38221 catggggtta tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct
    38281 ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg
    38341 aagcaggaat taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta
    38401 ttgatagcct gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga
    38461 cgctttaacg gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc
    38521 actgggtgta cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg
    38581 tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa
    38641 gctcggcacc gaatatgtta tcgccctgat cccgctgggc ggttatgtca aaatgctgga
    38701 tgagcgcgca gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt
    38761 tggccaacga gcggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt
    38821 tgcctactgg ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggtgaaat
    38881 agcagccaat tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt
    38941 agatggtatc gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg
    39001 cgatgaaagc accaccatta cagtagcgcc atttggcagc gatcaacggc gggatgtaaa
    39061 gctcgattta cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg
    39121 gattcgtcct cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc
    39181 ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt
    39241 aacgcagtgg gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt
    39301 agaaatcgaa aggcagggaa gtcccttgtc tttgacatta atcccggaga gtaaaccggg
    39361 taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga
    39421 gtataaagtt gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac
    39481 gtggcagctg atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa
    39541 actgaacaac ctcagtgggc cgatctctat cgccaagggg gctgggatga cagcggaact
    39601 cggggtagtt tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa
    39661 cctgtttccg ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat
    39721 caagggcgga ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct
    39781 gctggtgctg ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt
    39841 taggaagaac gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt
    39901 agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc
    39961 cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc cggtgcgcac aggcgacacg
    40021 gttaatgatg aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag
    40081 gatgttcgcg tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc
    40141 attgccagca ttactttctc cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac
    40201 ctcgaggctt ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc
    40261 gagaaaggtc tggaagactt ctactacagc gtcggtaaat atagcgccag cgtaaaagct
    40321 gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg
    40381 tcagctgaaa tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg
    40441 atctctcatt tccaactgcg tgacgaagtg ccgtggtgga acgtggtagg cgatcgtaaa
    40501 taccagaaac agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggatcgc
    40561 ggttatgccc gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt
    40621 atttacgtca cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg
    40681 agcggcaacc ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggt
    40741 gagctgtata acggcaccaa agtgaccaag atggaagatg acatcaaaaa gcttctcggt
    40801 cgctatggtt atgcctatcc gcgcgtacag tcgatgcccg aaattaacga tgccgacaaa
    40861 accgttaaat tacgtgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt
    40921 tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa
    40981 ggtgcatggc tggggagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc
    41041 ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga ccaggtagat
    41101 gtagtctaca aggtgaaaga acgtaacacc ggtagcttca acttcgggat tggttacggt
    41161 actgaaagtg gcgtgagctt ccaggctggt gtgcagcagg ataactggtt aggtacaggt
    41221 tatgctgttg gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta
    41281 accaacccgt acttcactgt agatggtgta agcctcggtg gtcgtctctt ctataatgac
    41341 ttccaggcag atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg
    41401 acgttgggct tcccgatcaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat
    41461 aactccctgt ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt
    41521 gaacatccga gcacctctga tcaggataat agcttcaaaa cggacgactt cacgttcaac
    41581 tatggttgga cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc
    41641 aacctgaccg gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta
    41701 gacacggcga cttatgtgcc gatcgatgac gatcacaaat gggttgttct ggggcgtacc
    41761 cgctggggtt atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat
    41821 gccggtggtt ccagcaccgt gcgtggcttc cagtccaata caattggtcc gaaagcagtt
    41881 tacttcccgc atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag
    41941 gacggcgcga aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt
    42001 gccagcctcg agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt
    42061 cgtacttcct tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa
    42121 tattctggat atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca
    42181 ttacaatgga tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag
    42241 tacgatggag acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc
    42301 tccacaaagg aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc
    42361 gggccacgca aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa
    42421 aaagtggtta ttagctgcag gtctcggttt agcactggca acttctgctc aggcggctga
    42481 caaaattgca atcgtcaaca tgggcagcct gttccagcag gtagcgcaga aaaccggtgt
    42541 ttctaacacg ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac
    42601 cgatctgcag gctaaaatga aaaagctgca gtccatgaaa gcgggcagcg atcgcactaa
    42661 gctggaaaaa gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga
    42721 gcaggatcgc gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac
    42781 tgctgtgaaa tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgccgt
    42841 tgcttacaac agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata
    42901 agtaatgcct tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg
    42961 tgatggcgat atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat
    43021 tacgttcatg gttaacccaa aataccgtga gcatttaggc ttgtgccagg cgtccgcggt
    43081 tgtcatgacc caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc
    43141 ctacctgact tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa
    43201 cattgcaccc agtgcggtga tcgacgcgac ggcaaagctg ggtaacaacg tatcgattgg
    43261 cgctaacgcg gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg
    43321 ttgcttcgta ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac
    43381 catttaccat gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg
    43441 cgcagacggc tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg
    43501 tcgcgtaatt attggcgatc gcgtggagat cggtgcctgc acaaccatcg atcgcggcgc
    43561 gctggatgac actgttattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca
    43621 taacgtcgtg attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct
    43681 gaaaattggt cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat
    43741 atgcgacaaa gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg
    43801 cgtctattcc tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact
    43861 ggtgatgaac attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca
    43921 acaagactaa cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat
    43981 tgcggcaggc cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact
    44041 actaacactc atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg
    44101 ttcttactgg tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa
    44161 aatgtctctg tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg
    44221 ggtgtgctga ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta
    44281 ggaaaactgg aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag
    44341 cgcccggtcg tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc
    44401 ggcctgaccc gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg
    44461 atgatgtgtg ctcgtagccg ggaggcctga taagtgattg ataaatccgc ctttgtgcat
    44521 ccaaccgcca ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt
    44581 tgtatcgttg gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc
    44641 gtgaatggtc atactaaaat tggccgcgat aatgagattt atcagttcgc ctccatcggc
    44701 gaagttaacc aggatctgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt
    44761 aaccgcattc gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg
    44821 aaggtgggca gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta
    44881 ggtaaccgct gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac
    44941 ttcgcgatca tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcacgtg
    45001 atggttggcg gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt
    45061 aaccacgcaa cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt
    45121 gaggcgatta ccgctatccg caatgcgtat aagctgattt atcgtagcgg taaaacgctc
    45181 gatgaagtga aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt
    45241 accgatttct ttgcacgctc aacgcgcggt ctgattcgtt aatgactgaa cagcgtccat
    45301 taacgattgc cctggtcgcc ggagaaacct ccggcgatat cctgggggcc ggtttaatcc
    45361 gcgctctgaa agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc
    45421 aggctgaagg ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg
    45481 aagtgctcgg tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg
    45541 gcgaactgaa gccagatatt tttgttggta ttgatgcgcc tgacttcaat attactcttg
    45601 aaggtaacct caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg
    45661 cgtggcgaca gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc
    45721 tgcctttcga aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata
    45781 ccatggctga tgccatgccg ttagatccag ataaaaatgc cgcccgtgat gtgctgggga
    45841 tccctcacga tgcccactgc ctggcgttgc taccagggag ccgtggtgca gaagttgaaa
    45901 tgcttagtgc cgatttcctg aaaacggccc agcttttgcg ccagacatat ccggatctcg
    45961 aaatcgtggt gccactggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg
    46021 aagtcgcgcc agacctttca gttcatttgc tggatgggat gggccgtgag gcgatggtcg
    46081 ccagcgatgc ggcgctactg gcgtcgggta cggcagccct ggagtgtatg ctggcgaaat
    46141 gcccgatggt ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg
    46201 tgaaaactga ttatgtctcg ctgccaaatc tgctggcggg cagagagtta gtcaaagaat
    46261 tattgcagga agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga
    46321 acgggaaaac cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct
    46381 gcaatgccga tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt
    46441 ttatccgcac acgcagctgg ttgcgggtgt ggatgaagtc ggacgtgggc cgttagttgg
    46501 cgcggtcgtc accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga
    46561 ttccaaaaag ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc
    46621 gttgagctgg agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca
    46681 tgcgaccatg ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt
    46741 gttgattgat ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg
    46801 cgatagccgc gtaccggaaa tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc
    46861 cgaaatggcg gcgctggata ttgttttccc gcaatatggt tttgcccaac acaaagggta
    46921 cccaaccgct tttcatctgg aaaaactggc tgaacacggc gcgaccgaac accatcggcg
    46981 cagctttggg cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta
    47041 agtaaaccgg aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga
    47101 ctactcgatg atcgatggcc tggccaaaac cgcaccgttg gtaaaaaagg cggcggcgtt
    47161 gggtatgcca gcactggcga tcaccgattt caccaacctt tgtggtctgg tgaagttcta
    47221 cggagcggga catggcgcag ggattaagcc tatcgtcggg gcagatttta acgtccagtg
    47281 cgacctgctg ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta
    47341 tcagaatctg acgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc
    47401 gatcatcgat cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg
    47461 acgcatgggc gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg
    47521 tgtcgcgttt tatgaagaac acttcccgga tcgctatttt cttgagctga tccgcaccgg
    47581 caggccggat gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt
    47641 gcccgtcgtg gcgaccaacg acgtgcgctt tatcgacagc agcgactttg acgcacacga
    47701 aatccgcgtc gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta
    47761 ttcgccgcag caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc
    47821 cgaagccctt gccaacaccg ttgagatcgc caaacgctgt aacgtaaccg tgcgtcttgg
    47881 tgaatacttc ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa
    47941 gcgtgcaaaa gagggcctgg aagagcgtct ggccttttta ttccctgatg aggaagaacg
    48001 tcttaagcgc cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca
    48061 gatgggcttc ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa
    48121 cggcgtaccg gtagggccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct
    48181 gaaaatcacc gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc
    48241 ggaacgtgtc tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt
    48301 tatcgagcac gtagcggaca tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg
    48361 tacgatggcg gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg
    48421 ctttgtcgat cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc
    48481 gtttgaagcc gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct
    48541 gatcgacatg gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg
    48601 ggtggttatc gcaccgacca aaattaccga ttttgcgccg ctttattgcg atgaagaggg
    48661 caaacatccg gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt
    48721 cgacttcctt ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa
    48781 gcggcgggcg aagaatggcg agccgccgct ggatatcgcc gcgatcccgc tggatgacaa
    48841 gaaaagcttc gacatgctgc aacgctcgga aaccactgcg gtattccagc ttgaatcgcg
    48901 cggcatgaag gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgccct
    48961 ggtggcactg ttccgccccg gtccgttgca atcagggatg gtggataact ttatcgaccg
    49021 taaacatggt cgtgaagaga tctcctatcc ggacgtgcag tggcagcatg aaagcttgaa
    49081 accggtactg gagccaacct acggcatcat cctgtatcag gaacaggtca tgcagattgc
    49141 ccaggtgctt tctggttata ccctcggtgg cgcggatatg ctgcgtcgtg cgatggggaa
    49201 gaaaaagccg gaagagatgg ctaagcaacg ttctgtattt gctgaaggtg cagaaaagaa
    49261 cggaatcaac gctgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgctggtta
    49321 cggatttaac aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct
    49381 gaaagcacac tatcctgcgg agtttatggc ggcggtaatg accgccgata tggacaacac
    49441 cgagaaggtg gtgggtctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc
    49501 agatataaac tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg
    49561 tattggcgcg atcaaagggg tcggtgaagg tccgattgag gccatcatcg aagcccgtaa
    49621 taaaggcggc tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt
    49681 gaaccgtcgc gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca
    49741 tcgcgcggcg ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa
    49801 agcggaagct atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat
    49861 tgaacaatcc tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg
    49921 tgaaacgtta ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga
    49981 gcgttatgtc ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat
    50041 cacggctgcg gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat
    50101 cggtatctgc acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc
    50161 cctggataaa taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt
    50221 cagctttgat gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga
    50281 cgaagcccgg gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga
    50341 tgaccagctt ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc
    50401 agtacatctc tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg
    50461 gcgtgtctct ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca
    50521 ggtggaactg gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga
    50581 acagccgatt gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga
    50641 tgagaaactg gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga
    50701 actgacacgt aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca
    50761 tccacagcgt ccttataccc tggattacgt tcgcctggca tttgatgaat ttgacgaact
    50821 ggctggcgac cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtcttga
    50881 cggtcgtccg gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg
    50941 ccgtaacttt ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat
    51001 ggctgaacgc tttaagatgc caatcatcac ctttatcgac accccggggg cttatccggg
    51061 cgtgggcgca gaagagcgcg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc
    51121 tcgccttagc gtaccgacta tttgtaccgt tatcggtgaa ggtggttctg gcggcgcgct
    51181 ggcgattggc gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc
    51241 gccggaaggt tgtgcgtcca ttctgtggaa gagcgctgat aaagcgccgc tggcggctga
    51301 agcgatgggt atcatcgctc cgcgtctgaa agaactaaaa ctgatcgact ccatcatccc
    51361 ggaaccgctg ggtggtgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca
    51421 actgctggcg gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg
    51481 ttatcagcgc ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca
    51541 cttcggtggc ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc
    51601 caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat
    51661 gagcccatca aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca
    51721 caaaacagcg ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt
    51781 tttgactggg atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat
    51841 ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg
    51901 cggatggcgc tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt
    51961 atgcgtcagt acaccgacga atatcttgat aacattacac cgccgttcac gaaagccttg
    52021 tttacctacg tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc
    52081 gcatatcaaa aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc
    52141 aaggctgatg tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca
    52201 cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt
    52261 accaacggaa catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt
    52321 acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat
    52381 gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg
    52441 cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa
    52501 tggccggttc atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac
    52561 tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac
    52621 acccattttc atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga
    52681 aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg
    52741 ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat
    52801 accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg
    52861 cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc
    52921 aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg
    52981 ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt
    53041 aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg
    53101 gggatggacg aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa
    53161 ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc
    53221 tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt
    53281 aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa
    53341 gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg
    53401 attcgtaaac acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg
    53461 atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg
    53521 ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta
    53581 ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta
    53641 ctgatgcttt gttccgtagt gcaacattac cccggttttg aaacggatat tcacggcgcg
    53701 aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc
    53761 agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt
    53821 taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct
    53881 actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt
    53941 ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc
    54001 cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg
    54061 atgacatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc
    54121 gtgtcgatcc atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt
    54181 tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca
    54241 ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca
    54301 cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg
    54361 gtgcagtggc ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt
    54421 ttaagtgcca atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag
    54481 gtgccgctgg tggtcgaacg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag
    54541 gcgcggcagg cgcgttatca ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc
    54601 accgcgcaac atctcgacga tcaatgtgaa acttttctgc tggcgctaaa acgcggtagc
    54661 ggccctgccg ggctttcggc tatggcggaa gtttcggagt ttgccggaac acggcttatc
    54721 cgcccgttgc tcgcccgcac gcggggagaa ctggagcagt gggcattggc gcatggttta
    54781 cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg
    54841 cgcgtagtgc cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc
    54901 gccgcacttt gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca
    54961 cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc
    55021 cgccgcgcgg cgattatccg tcgctggctg gcagggcaga atgcaccaat gccttcccgc
    55081 gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt
    55141 ttacgcttgg gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc
    55201 gtcaccgggc aaagtgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta
    55261 ccggcggggc tgggaagcgt acagcttact gcgggaggcg atattcgccc tccgcgtgca
    55321 gacgaagcgg tcagcgtgcg tttcaaagcg gcaggattgc tgcatattgt cgggcgcaac
    55381 ggcggacgta agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac
    55441 accacgccac tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg
    55501 caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt
    55561 taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtta atcaggactc
    55621 gctcaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag
    55681 ctcacgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
    55741 atcgctggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
    55801 atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc
    55861 attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
    55921 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
    55981 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
    56041 actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag
    56101 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
    56161 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
    56221 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
    56281 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
    56341 tatgatgctg cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
    56401 tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
    56461 tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc
    56521 cgcagaagtg gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa
    56581 tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact
    56641 ggcgaaagta agcgtctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
    56701 cgtgatctgg ttatcggacg ataagaataa tctggaagtt aacttaaccg cctggcaaca
    56761 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
    56821 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat
    56881 ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg tctactcgcc
    56941 gccagccatc atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc
    57001 agtcaggaac tgaaccgcga agccgcgcta gcccggctgg tggcagtgat taaagattta
    57061 acaacagaac aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg
    57121 ctggcatcga aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt
    57181 cgggaataaa aagaaggaat agatggtgaa aaaagcgata gtgacagcga tggctgtaat
    57241 cagcctcttt actctgatgg gatgtaataa ccgggccgaa gtcgatacgc tttctccagc
    57301 acaggctgcc gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgctga
    57361 ttgcgaagga atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga
    57421 gcgttatctc ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg
    57481 aaccgctgac aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa
    57541 aggcgatgcg ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta
    57601 tacgctggaa ccggcacaat ctagtttacc tatgacgccg atgaccctgc gaggcatgta
    57661 tttttatatg gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt
    57721 agcgaataac gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc
    57781 ggtgttactg tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc
    57841 aaccaaagta ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag
    57901 tttggggcag taaacctgct taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc
    57961 ttatcaggcc tacagcatat aatgcaacat tttgaattag cacgattttg taggccggat
    58021 aaggcgttta cgccgcatcc ggcattttgt gcacgaggct ggctttatca ctgagtcagc
    58081 ccaacaaagc gacccgcctt gatataaatc ccaggcttca ggtgcccaac atactgtaac
    58141 tgcgtttctg gcttaaacgc agagacatca ccaatacgca tatgcatcag atcggcaggg
    58201 ctaatccagc ccgattgcgc cagtgtaagt ggatgcccgg ctttggcaaa ggcatccgtg
    58261 acaaattccg aacaaaacca cgactttttg tctccttcgc ccacactgct taactgcgct
    58321 ttcgccaggc cgctgacgca ctttggcgaa aatcctcgga gaacggattc agtgagcaca
    58381 tctggcgagt caccataaag ggaataaatt cgacaatgcc gcgatagtta taaccgctat
    58441 ctttgatttt attggcaaac gcggtgatat ctgtggcttg ttgcggggta agatccggga
    58501 ctcgtaagac gaaaagctta tcactatgct tcatcgcttt tttaagggaa acaatctgga
    58561 cgccagcgcc tgtcgcttct gcaacgttat tatcacccag aaagattgca acgtgactca
    58621 cagaggaagt gctgaagacg cggattccaa atgaggttac cccaaggctt gaggagaaca
    58681 gcaaatcgcc gggtttgaga tcaggtgccg ttatttcttt tattgattgt tcggtgaaag
    58741 agctttgatg ctggaatttg acagcccatg tttttgcctc ggcatctact gctgtggctg
    58801 atgggtctgg ctggctgata tcaacggtac aagccgaaag taaaagaaag ctggggagaa
    58861 acaggcggca gtacgccttt ggtttatcca ttttatacaa tccatgtaaa aaaagggccc
    58921 tgaaattcag gaccctttct ggcatcagcc tttaatctgt ttcaccagat attcgacgat
    58981 gtcaccagtc ttaattaact gtttctcgcc gttgcgacga tatttatatt cgatatcgtc
    59041 gttgtcgagg ttacggtcgc ccagcacaat agtgtgcgga ataccgatca gttccatatc
    59101 agcaaacatc acgcccggac gctctttgcg gtcatccagc agcacttcga taccttgtgc
    59161 acgcagttcg ctgtacagtt tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt
    59221 catcggcaga atcgccacct ggaacggcgc gatagcgtca ggccatacga tgcctcgttc
    59281 gtcgtagttc tgctcaatcg ccgcagctac cacacgcgtt accccgatac cgtagcaacc
    59341 catcgtcagg atttggttac ggccatcttc accctgtacg gaggctttca gtgcttcgga
    59401 gtacttggta cccagctgga agatgtgacc aacttcgata ccacgtttga tcagcagcgt
    59461 accctggcca tccgggcttg gatcgccagc caccacgtta cggatatctg caacttccgg
    59521 ggtagcgaca tcgcgatccc agttgatgcc gaagtagtgt ttaccatcga tgttagcacc
    59581 agcagcgaaa tcactcatcg ccgcaacggt acggtcaatc accaccggaa tcggcatgtt
    59641 taccggaccc agtgaacccg gaccggcttt aaccacggca cgaatttctt cttcggtcgc
    59701 gaaagtcagc gggcttgcaa cctgcggcag tttttctgct ttaacttcgt tcagctcgtg
    59761 atcaccgcgc accagcagcg caaccagcgg gaagctactg ccttcaaccg ctttaaccag
    59821 cagagtctta accgttttct caatcggcag attgaactgt tcaaccagtt ccgcgatggt
    59881 tttcgcgttc ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt
    59941 cggcgcgata gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt cggagaagac
    60001 cacatcgtct tcaccgctct gcgccagcac ctggaattcg tgagaggcgc tgccgccgat
    60061 agaaccggtg tcggcttgta cggcgcggaa atccagcccc atgcggctga agattttgct
    60121 gtaggccgca tacattgcat cgtaggtttc ctgcagggat tcctgagaag tatggaaaga
    60181 gtaagcatct ttcatcagga attcgcggga acgcatgacg ccgaaacgcg gacgcacttc
    60241 gtcgcggaac ttggtctgga tctgatagaa gttcagcggc agctgtttgt aagagctaag
    60301 ctcgttacga atcaggtcag tgataacttc ttcatgagtt gggccgagta cgaacggacg
    60361 ctcgccacgg tcaacaaaac gcagcagttc cggaccgtac tgttcccaac gaccactctc
    60421 ttgccacaaa tcagctggct gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt
    60481 catctcttca cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg tcggcagcca
    60541 ggtatataac ccggaggcca gcttgcggat catcccggcg cgcagcatca gctgatggct
    60601 gatcacctcg gcgtcggcag gtgtctcctt gagagtggag agcaggtatt ggctagtacg
    60661 catgttgtta cggttccagt tggaaggtag aacaggctca aggcgagcct gggacaaaaa
    60721 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
    60781 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
    60841 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgtgg
    60901 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
    60961 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgccg ctggcgcact
    61021 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
    61081 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
    61141 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
    61201 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
    61261 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
    61321 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
    61381 gccgttggcg ctttttacca gacctggctg gcgcggaacg gcgaattttt ctttataggg
    61441 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
    61501 gcagagccga tacatacagc ctgacgatag cagcctggcg taccgctggt gacttcgcag
    61561 ctatgcagta ataccgcatt ggccttcatt ttcgaggcgt tgatttgcat ccgcttacgc
    61621 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    61681 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    61741 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    61801 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    61861 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    61921 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    61981 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    62041 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    62101 tctttgttat cttcacgcgt tacgatcagg tttacgtacg gggagtcttt atcttcgaca
    62161 aagataccgt ctttagctgg agtcaggcca atctggctgg cataggtggt attgataact
    62221 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    62281 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    62341 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    62401 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    62461 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    62521 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    62581 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    62641 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    62701 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    62761 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    62821 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    62881 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    62941 ttcatcaccg tcgcgttata gccgatgtag ccatactgat agccaatctg acctaaacca
    63001 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    63061 gccgcattca ccagacccgg caatgcttcc ggtaacagca ctttacggac gatctgcatc
    63121 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    63181 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aatcgctgcc
    63241 tgcaaaccaa tcgatgtacc aacaataacg cgggtaaacg gaatcatcca taccagcaag
    63301 ataatgaacg ggatagaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    63361 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    63421 atcacaaagc caaaaaaacc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    63481 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    63541 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    63601 gatgccgaac ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    63661 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgactgac cggtaaactc
    63721 cagacgcagc atcggcacgc agtcagtgaa tggctccgct tgcagacgtt cctggtaatc
    63781 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccaacg gcgttttcgg
    63841 atgcgaaaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    63901 cacgcaatca caaatacgct ttacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    63961 acccagacgg cggttaatgt ctttcagcag ctcgagaata gaacgtgtcg ttgccgggtc
    64021 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta acgcacgggc
    64081 aatcgccaca cgctgtttct gcccaccgga aagattcgat gggtagctat catgcttatc
    64141 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    64201 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    64261 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    64321 cgtggtcagt tcctggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    64381 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    64441 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    64501 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    64561 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    64621 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    64681 cgatactagc gtcacatgcc ttattaagga gctataaaag gtggcaaaga gcgtacccgc
    64741 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    64801 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    64861 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    64921 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    64981 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    65041 cgactgccgt aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    65101 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggctgcggc
    65161 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacgcctg aagcagaaaa
    65221 cgcggcggat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    65281 aaaaccggca taatgattaa aagatgagca attgaaataa aaatgcattt ttccacttgt
    65341 cttcctgaga c
//
LOCUS       DAZFWP020000004        65251 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFWP020000004 DAZFWP020000000
VERSION     DAZFWP020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.004"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            112..2751
                     /gene="ydbH"
                     /locus_tag="ACHK0Z_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             112..2751
                     /gene="ydbH"
                     /locus_tag="ACHK0Z_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU4500303.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2748..2933
                     /gene="ynbE"
                     /locus_tag="ACHK0Z_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     CDS             2748..2933
                     /gene="ynbE"
                     /locus_tag="ACHK0Z_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU4500304.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2938..3267
                     /gene="ydbL"
                     /locus_tag="ACHK0Z_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             2938..3267
                     /gene="ydbL"
                     /locus_tag="ACHK0Z_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU4500305.1"
                     /translation="MMKKTLLLCAFLVGLVSSNIMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(3439..4344)
                     /gene="feaR"
                     /locus_tag="ACHK0Z_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             complement(3439..4344)
                     /gene="feaR"
                     /locus_tag="ACHK0Z_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HGU4500306.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            4580..6079
                     /gene="feaB"
                     /locus_tag="ACHK0Z_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             4580..6079
                     /gene="feaB"
                     /locus_tag="ACHK0Z_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HGU4500307.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            complement(6137..8410)
                     /gene="tynA"
                     /locus_tag="ACHK0Z_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             complement(6137..8410)
                     /gene="tynA"
                     /locus_tag="ACHK0Z_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HGU4500308.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPTKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(8658..10703)
                     /gene="paaZ"
                     /locus_tag="ACHK0Z_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             complement(8658..10703)
                     /gene="paaZ"
                     /locus_tag="ACHK0Z_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HGU4500309.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELLPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDITPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQTLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            10988..11917
                     /gene="paaA"
                     /locus_tag="ACHK0Z_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     CDS             10988..11917
                     /gene="paaA"
                     /locus_tag="ACHK0Z_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HGU4500310.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWKEGTWVREAALAHAQKQHARKVA"
     gene            11929..12216
                     /gene="paaB"
                     /locus_tag="ACHK0Z_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             11929..12216
                     /gene="paaB"
                     /locus_tag="ACHK0Z_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HGU4500311.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            12225..12971
                     /gene="paaC"
                     /locus_tag="ACHK0Z_RS01140"
                     /old_locus_tag="pgaptmp_000228"
     CDS             12225..12971
                     /gene="paaC"
                     /locus_tag="ACHK0Z_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HGU4500312.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            12986..13483
                     /gene="paaD"
                     /locus_tag="ACHK0Z_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             12986..13483
                     /gene="paaD"
                     /locus_tag="ACHK0Z_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HGU4500313.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            13491..14561
                     /gene="paaE"
                     /locus_tag="ACHK0Z_RS01150"
                     /old_locus_tag="pgaptmp_000230"
     CDS             13491..14561
                     /gene="paaE"
                     /locus_tag="ACHK0Z_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HGU4500314.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            14558..15325
                     /gene="paaF"
                     /locus_tag="ACHK0Z_RS01155"
                     /old_locus_tag="pgaptmp_000231"
     CDS             14558..15325
                     /gene="paaF"
                     /locus_tag="ACHK0Z_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HGU4500315.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            15325..16113
                     /gene="paaG"
                     /locus_tag="ACHK0Z_RS01160"
                     /old_locus_tag="pgaptmp_000232"
     CDS             15325..16113
                     /gene="paaG"
                     /locus_tag="ACHK0Z_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HGU4500316.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLECDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            16115..17542
                     /gene="paaH"
                     /locus_tag="ACHK0Z_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             16115..17542
                     /gene="paaH"
                     /locus_tag="ACHK0Z_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HGU4500317.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRTLEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            17532..17954
                     /gene="paaI"
                     /locus_tag="ACHK0Z_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             17532..17954
                     /gene="paaI"
                     /locus_tag="ACHK0Z_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HGU4500318.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            17954..19159
                     /gene="paaJ"
                     /locus_tag="ACHK0Z_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             17954..19159
                     /gene="paaJ"
                     /locus_tag="ACHK0Z_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HGU4500319.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            19186..20499
                     /gene="paaK"
                     /locus_tag="ACHK0Z_RS01180"
                     /old_locus_tag="pgaptmp_000236"
     CDS             19186..20499
                     /gene="paaK"
                     /locus_tag="ACHK0Z_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HGU4500320.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            20600..21550
                     /gene="paaX"
                     /locus_tag="ACHK0Z_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             20600..21550
                     /gene="paaX"
                     /locus_tag="ACHK0Z_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HGU4500321.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            21532..22122
                     /gene="paaY"
                     /locus_tag="ACHK0Z_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             21532..22122
                     /gene="paaY"
                     /locus_tag="ACHK0Z_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HGU4500322.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHMGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            22354..23214
                     /gene="pdxI"
                     /locus_tag="ACHK0Z_RS01195"
                     /old_locus_tag="pgaptmp_000239"
     CDS             22354..23214
                     /gene="pdxI"
                     /locus_tag="ACHK0Z_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU4500323.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            23278..25584
                     /gene="ydbD"
                     /locus_tag="ACHK0Z_RS01200"
                     /old_locus_tag="pgaptmp_000240"
     CDS             23278..25584
                     /gene="ydbD"
                     /locus_tag="ACHK0Z_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU4500324.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            25755..26360
                     /gene="ynbA"
                     /locus_tag="ACHK0Z_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             25755..26360
                     /gene="ynbA"
                     /locus_tag="ACHK0Z_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4500325.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            26360..27256
                     /gene="ynbB"
                     /locus_tag="ACHK0Z_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             26360..27256
                     /gene="ynbB"
                     /locus_tag="ACHK0Z_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4500326.1"
                     /translation="MLEKSLATLFALLILATLINHFLVWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            27272..29029
                     /gene="ynbC"
                     /locus_tag="ACHK0Z_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             27272..29029
                     /gene="ynbC"
                     /locus_tag="ACHK0Z_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4500327.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            29019..30335
                     /gene="ynbD"
                     /locus_tag="ACHK0Z_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             29019..30335
                     /gene="ynbD"
                     /locus_tag="ACHK0Z_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU4500328.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(30386..30991)
                     /gene="azoR"
                     /locus_tag="ACHK0Z_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             complement(30386..30991)
                     /gene="azoR"
                     /locus_tag="ACHK0Z_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU4500329.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            31192..35094
                     /gene="hrpA"
                     /locus_tag="ACHK0Z_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             31192..35094
                     /gene="hrpA"
                     /locus_tag="ACHK0Z_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU4500330.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            35366..36166
                     /gene="ydcF"
                     /locus_tag="ACHK0Z_RS01235"
                     /old_locus_tag="pgaptmp_000247"
     CDS             35366..36166
                     /gene="ydcF"
                     /locus_tag="ACHK0Z_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU4500331.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            36363..37802
                     /gene="aldA"
                     /locus_tag="ACHK0Z_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     CDS             36363..37802
                     /gene="aldA"
                     /locus_tag="ACHK0Z_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4500332.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(37844..38845)
                     /gene="gap"
                     /locus_tag="ACHK0Z_RS01245"
                     /old_locus_tag="pgaptmp_000249"
     CDS             complement(37844..38845)
                     /gene="gap"
                     /locus_tag="ACHK0Z_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4500333.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            39034..39564
                     /gene="cybB"
                     /locus_tag="ACHK0Z_RS01250"
                     /old_locus_tag="pgaptmp_000250"
     CDS             39034..39564
                     /gene="cybB"
                     /locus_tag="ACHK0Z_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU4500334.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            39809..39982
                     /locus_tag="ACHK0Z_RS01255"
                     /old_locus_tag="pgaptmp_000251"
     CDS             39809..39982
                     /locus_tag="ACHK0Z_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4500335.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            40008..40076
                     /gene="yncP"
                     /locus_tag="ACHK0Z_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             40008..40076
                     /gene="yncP"
                     /locus_tag="ACHK0Z_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU4500336.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(40054..40203)
                     /gene="hokB"
                     /locus_tag="ACHK0Z_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             complement(40054..40203)
                     /gene="hokB"
                     /locus_tag="ACHK0Z_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU4500337.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            40602..42242
                     /gene="trg"
                     /locus_tag="ACHK0Z_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             40602..42242
                     /gene="trg"
                     /locus_tag="ACHK0Z_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU4500338.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(42280..43203)
                     /gene="ydcI"
                     /locus_tag="ACHK0Z_RS01275"
                     /old_locus_tag="pgaptmp_000255"
     CDS             complement(42280..43203)
                     /gene="ydcI"
                     /locus_tag="ACHK0Z_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4500339.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            43420..44763
                     /gene="ydcJ"
                     /locus_tag="ACHK0Z_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             43420..44763
                     /gene="ydcJ"
                     /locus_tag="ACHK0Z_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4500340.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            44988..46643
                     /gene="opgD"
                     /locus_tag="ACHK0Z_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /gene_synonym="mdoD"
     CDS             44988..46643
                     /gene="opgD"
                     /locus_tag="ACHK0Z_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU4500341.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            46783..47007
                     /gene="ydcH"
                     /locus_tag="ACHK0Z_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             46783..47007
                     /gene="ydcH"
                     /locus_tag="ACHK0Z_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU4500342.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            47070..47606
                     /gene="rimL"
                     /locus_tag="ACHK0Z_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             47070..47606
                     /gene="rimL"
                     /locus_tag="ACHK0Z_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGU4500343.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(47601..48581)
                     /gene="ydcK"
                     /locus_tag="ACHK0Z_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     CDS             complement(47601..48581)
                     /gene="ydcK"
                     /locus_tag="ACHK0Z_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU4500344.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            48705..49697
                     /gene="tehA"
                     /locus_tag="ACHK0Z_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     CDS             48705..49697
                     /gene="tehA"
                     /locus_tag="ACHK0Z_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU4500345.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            49694..50287
                     /gene="tehB"
                     /locus_tag="ACHK0Z_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     CDS             49694..50287
                     /gene="tehB"
                     /locus_tag="ACHK0Z_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU4500346.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            50590..51258
                     /gene="ydcL"
                     /locus_tag="ACHK0Z_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             50590..51258
                     /gene="ydcL"
                     /locus_tag="ACHK0Z_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU4500347.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(51357..51719)
                     /gene="tnpA"
                     /locus_tag="ACHK0Z_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /pseudo
     CDS             complement(51357..51719)
                     /gene="tnpA"
                     /locus_tag="ACHK0Z_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            51789..52997
                     /gene="insQ"
                     /locus_tag="ACHK0Z_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             51789..52997
                     /gene="insQ"
                     /locus_tag="ACHK0Z_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4500348.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTPARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(53037..54251)
                     /gene="ydcO"
                     /locus_tag="ACHK0Z_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             complement(53037..54251)
                     /gene="ydcO"
                     /locus_tag="ACHK0Z_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGU4500349.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIFTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAGAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            54304..54840
                     /gene="sutR"
                     /locus_tag="ACHK0Z_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             54304..54840
                     /gene="sutR"
                     /locus_tag="ACHK0Z_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU4500350.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPATPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            54913..56874
                     /gene="rlhA"
                     /locus_tag="ACHK0Z_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             54913..56874
                     /gene="rlhA"
                     /locus_tag="ACHK0Z_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU4500351.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(56966..57196)
                     /gene="yncJ"
                     /locus_tag="ACHK0Z_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             complement(56966..57196)
                     /gene="yncJ"
                     /locus_tag="ACHK0Z_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU4500352.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            57418..57572
                     /locus_tag="ACHK0Z_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /pseudo
     CDS             57418..57572
                     /locus_tag="ACHK0Z_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            57618..58034
                     /gene="hicB"
                     /locus_tag="ACHK0Z_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     CDS             57618..58034
                     /gene="hicB"
                     /locus_tag="ACHK0Z_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU4500353.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            58113..59519
                     /gene="ydcR"
                     /locus_tag="ACHK0Z_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             58113..59519
                     /gene="ydcR"
                     /locus_tag="ACHK0Z_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4500354.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            59764..60909
                     /gene="ydcS"
                     /locus_tag="ACHK0Z_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             59764..60909
                     /gene="ydcS"
                     /locus_tag="ACHK0Z_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU4500355.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            60927..61940
                     /gene="ydcT"
                     /locus_tag="ACHK0Z_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             60927..61940
                     /gene="ydcT"
                     /locus_tag="ACHK0Z_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4500356.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            61941..62882
                     /gene="ydcU"
                     /locus_tag="ACHK0Z_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             61941..62882
                     /gene="ydcU"
                     /locus_tag="ACHK0Z_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4500357.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            62872..63666
                     /gene="ydcV"
                     /locus_tag="ACHK0Z_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             62872..63666
                     /gene="ydcV"
                     /locus_tag="ACHK0Z_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4500358.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLATAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            63688..65112
                     /gene="patD"
                     /locus_tag="ACHK0Z_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             63688..65112
                     /gene="patD"
                     /locus_tag="ACHK0Z_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU4500359.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT        16099 a        15512 c        17273 g        16367 t
ORIGIN      
        1 tcatcaacaa ctatgcttag tgtaggcgca accttcaact gaacggttaa acatgccaca
       61 atacccgtat tgaatgctta atttttcgct aaatcaggat attaactacc catgctgggt
      121 aaatataaag ccgttctcgc gctgttatta ctgattattc ttgtgccgtt gacgctgttg
      181 atgacgctcg ggctgtgggt tcccacgctg gcgggcatct ggctaccgct cgggacacgt
      241 attgcattag atgaaagccc acgcattacg cgtaaaggtt taatcattcc cgatctccgt
      301 tatctggtgg gagattgtca gcttgcgcat atcaccaacg ccagcctttc acatcccagc
      361 cgctggttat tgaacgtcgg cacggtagaa cttgattctg cttgtctggc gaaattgccg
      421 cagacggagc aatcgccagc tgctccaaaa accctcgcgc agtggcagtc catgctgcct
      481 aacacctgga tcaatatcga taaactgatt ttttctccct ggcaggaatg gcagggaaaa
      541 ctctctctcg cattaacctc tgatatccag caactgcgtt atcagggcga aaaagttaaa
      601 tttcaaggcc agctgaaagg gcaacaactt acagtcagcg aactggatgt cgtcgcgttt
      661 gaaaatcagc cgccggttaa actggtgggg gaatttacta tgccgctcgt gccggatgga
      721 cttcctgtaa gtgggcatgc tactgcgacg ttaaacttgc cgcaggaacc gtcactggtg
      781 gatgcagagc tggactggca ggaaaatagc gggcaattga ttgtgctggc acgggataac
      841 ggcgatccgt tgctcgattt gccgtggcaa attactcgtc aacaattgac cgtaagcgat
      901 ggtcgctgga gttggccgta tgcaggtttt cctttgagtg gccgactggg tgtcaaagtc
      961 gacaactggc aggcagggct tgagaacgct ctgatcagcg gacgactgag tgtgctgacc
     1021 caggggcaag cgggtaaagg caacgcggtg ctaaattttg gcccaggaaa attaagcatg
     1081 gataacagtc agctgcctct gcagctgacc ggtgaagcga aacaggcgga cctcatttta
     1141 tatgcccgtt tacctgcgca gctaagtgga agtctgtctg acccaacgct gacctttgag
     1201 ccaggcgcgt tacttcgttc aaagggaaga gtcatcgatt cgctggacat cgatgaaatc
     1261 cgctggcctt tagcgggtgt aaaagtcacc caacgtggtg ttgacggacg tttgcaggcc
     1321 attttgcagg cgcatgaaaa tgaactgggc gatttcgtgc tgcatatgga tgggctggcg
     1381 aatgattttc tccctgacgc gggccgctgg cagtggcgct actggggaaa aggcagtttt
     1441 acaccgatga atgccacctg ggatgtcgca ggaaaaggtg aatggcatga cagcacgatt
     1501 acgctgaccg acctctccac cggtttcgac cagttacaat acggtacgat gacggtagaa
     1561 aagccgcgat taattctcga caagcccgtc gtctgggtac gtgacgcaca gcatccctcc
     1621 ttcagcggcg cgctgtcact ggacgccggg caaacgctgt tcactggcgg cagtgtgtta
     1681 ccgccatcaa ccttaaaatt tagcgtcgat gggcgcgatc cgacgtattt cctctttaaa
     1741 ggcgatttac atgctggtga gattggcccg gttcgggtaa atggtcgctg ggacggtatt
     1801 cgtctgcgcg gtaacgcctg gtggccaaaa caatcactga ctgtattcca gccgctggtg
     1861 ccacccgact ggaagatgaa cttacgcgat ggtgaactgt atgctcaggt tgcattttct
     1921 gctgcgccgg aacaaggatt ccgcgcggga gggcacggcg tgttgaaagg cggtagtgcc
     1981 tggatgccag ataatcaggt taacggtgtc gattttgtcc tgcctttccg ttttgccgat
     2041 ggagcctggc atctggggac tcgcggcccc gttacgttgc gaattgccga agtgattaat
     2101 ctggtgacag cgaaaaatat tacggctgat ttgcaagggc gttatccgtg gactgaagaa
     2161 gaacccttgc tgttgactga tgttagcgtc gatgtgttag gcggtaacgt actgatgaaa
     2221 caattacgta tgccgcaaca tgacccggcg ctgttgcggc tgaataatct ctcatccagc
     2281 gaactggtta gcgccgtcaa tccgaaacaa ttcgccatgt ccggggcatt tagtggtgca
     2341 ttgccattat ggctaaacaa cgaaaaatgg atagtgaaag atggttggct ggcaaatagc
     2401 gggccaatga ccttgcggct ggataaagac accgcagatg cggtggtgaa agacaatatg
     2461 actgcgggtt cagcaattaa ctggttgcgc tatatggaaa ttagccggtc atcgacaaaa
     2521 attaacttag ataatctcgg tttattaacc atgcaggcca acattacagg taccagtcgc
     2581 gttgatggta aaagcggtac ggtaaacctt aattaccatc atgaagagaa tatttttacg
     2641 ctgtggcgca gtttacgctt tggcgataat ctccaggcat ggttggagca gaacgcacgt
     2701 ctgccgggaa atgactgtcc gcaaggaaaa gagtgtgagg aaaaacaatg aaaattttac
     2761 tggctgcgtt gacgtcatct tttatgctgg ttggctgtac gcctcgcatt gaagtcgctg
     2821 cacctaagga accgatcact atcaatatga acgttaaaat tgagcatgag atcatcatca
     2881 aggcagacaa agatgtcgaa gagctgcttg aaactcgtag cgatcttttc tgaggcgatg
     2941 atgaagaaaa cattacttct ttgtgcgttt cttgttgggc tggtaagcag caatataatg
     3001 gcattgactc tggatgaagc cagaactcag gggcgggtag gtgaaacatt ttatggttat
     3061 ctggttgcgc tgaaaacgga tgctgaaaca gagaaattag taaccgacat taatgccgaa
     3121 cgtaaagcga gttaccaaca actggcaaag caaaataatg tgtcggtaga tgatatcgcg
     3181 aaactcgccg ggcaaaagct cgtagctcgg gccaaaccgg gggaatatgt acaagggata
     3241 aacggtaaat gggtgcgaaa attttaatag caaaaccgca atatcgggat aatgctacag
     3301 gagaaagcga ttgatctatt ttcctgaaac aaggtgaata ttaaaaaact cctgttaaat
     3361 tgccttttgc cctgaaaaat gcatagtcaa atatctgttt taactaattg gcgttgcagt
     3421 acatgcaacg cgaattagtt agcggaattt acgccgatac tcgcctggcg tcatcccaaa
     3481 gcgttgctta aatactgttg aaaaatgact ctggtcggaa aatccccaat gaaaaccaat
     3541 gcctgccagt ttttcatcat ctgcggcatg gcgaatcgca tcagcacaaa aatcgagacg
     3601 acggttacga atatattgcg cgaccaccaa acctttatcg gcaaacattc ggtacaaact
     3661 acgtactgac atacctgtct ctccggctat ccactccggg cgtaatatct cttcgcgaat
     3721 attatcgtct atcagcgtaa ccactttttg aaactgacgt tcacggcgag gttgaacaga
     3781 ttcccgctga tgaagtaccg ggcgcagcag acacaccatc gcctgtagcg cagcttcact
     3841 ttctgtttca gaaagtgccg ggttattcat gctctcctgt aacaggcgat gactgagttg
     3901 caccatgggt aagtcagcgt ccagtctttc tgcgcagaca ggtttttgat ggggaaaata
     3961 ttgttccagc agagtgcgtg gtaaaagtaa tgaaatctgt ttagaagact cctgccagta
     4021 aagcgaacag gggcgtgagg catcgagtaa cgtaatatcg ccagcgccaa tctgcacctg
     4081 acgctcatcc tgctccatta ttgcctgacc actaagttga aaaacggtgt aaaaccaggc
     4141 atcgtcgctg ccttttacct cctgccaggt gcggtataaa ttcactccgt tggttgtcac
     4201 ggtgctcagc tttagtcctt tggcaaaatg cgtttccagt acacccgtgt aacgctcagt
     4261 cagcaggcgt ccggtaaaat ttccgcatac ctgattgatt tgggaaagcc attgctgaaa
     4321 ctcattatcc actgcggggt tcatggcacg ttttcgctct gtgaaatgta tttttattgt
     4381 tgcatttgtg ttgcaataaa cgaagctaat gagcctgact ataggaaata agtcttgtca
     4441 ggcatagaga cataagcggt tattgtcacg atttgcggag cttgtcacag ctgacaaagc
     4501 gaatgtcaca gcgaaaaaag tgacttttct tgtcgctgcg tacactgaaa tcacactggg
     4561 taaataataa ggaaaagtga tgacagagcc gcatgtagca gtattaagcc aggtccaaca
     4621 gtttctcgat cgtcaacacg gtctttatat tgatggtcgt ccaggccccg cacaaagtga
     4681 aaaacgattg gcgatctttg atccggccac cgggcaagaa attgcgtcta ctgctgatgc
     4741 caacgaagcg gatgtagata acgcagtcat gtctgcctgg cgggcctttg tctcgcgtcg
     4801 ctgggccggg cgattacccg cagagcgtga acgtattctg ctacgttttg ccgatctggt
     4861 ggaacagcac agtgaggagc tggcgcaact ggaaaccctg gagcaaggca agtcaattgc
     4921 catttcccgt gcttttgaag tgggctgtac gctgaactgg atgcgttata ccgccgggtt
     4981 aacgaccaaa atcgcgggta aaacgctgga cttgtcgatt cccttacccc agggggcgcg
     5041 ttatcaggcc tggacgcgta aagagccggt tggcgtagtg gcgggaattg tgccatggaa
     5101 ctttccgttg atgattggta tgtggaaggt gatgccagca ctggcagcag gctgttcaat
     5161 cgtgattaag ccttcggaaa ccacgccact gacgatgttg cgcgtggcgg aactggccag
     5221 cgaggctggt atccctgatg gcgtttttaa tgtggtcacc gggtcaggtg ctgtatgcgg
     5281 cgcggccctg acgtcacatc ctcatgttgc gaaaatcagt tttaccggtt caaccgcgac
     5341 gggaaaaggt attgccagaa ctgctgctga tcacttaacg cgtgtaacgc tggaactggg
     5401 cggtaaaaac ccggcaattg tattaaaaga tgctgatccg caatgggtta ttgaaggctt
     5461 gatgaccgga agcttcctga atcaagggca agtatgcgcc gccagttcgc gaatttatat
     5521 tgaagcgccg ttgtttgaca cgctggttag tggatttgag caggcggtaa aatcgttgca
     5581 agtgggaccg gggatgtcac ctgttgcaca gattaaccct ttggtttctc gtgcgcactg
     5641 cgacaaagtg tgttcattcc tcgacgatgc gcaggcacag caagcagagc tgattcgcgg
     5701 gtcgaatgga ccagccggag aggggtatta tgttgcgcca acgctggtgg taaatcccga
     5761 tgctaaattg cgcttaactc gtgaagaggt gtttggtccg gtggtaaacc tggtgcgagt
     5821 agcggatgga gaagaggcgt tacaactggc aaacgacacg gaatatggct taactgccag
     5881 tgtctggacg caaaatctct cccaggctct ggaatatagc gatcgcttac aggcagggac
     5941 ggtgtgggta aacagccata ccttaattga cgctaactta ccgtttggtg ggatgaagca
     6001 gtcaggaacg ggccgtgatt ttggccccga ctggctggac ggttggtgtg aaactaagtc
     6061 ggtgtgtgta cggtattaat ctggttcgct cataagtaaa aaacggcacc tggtgccgtt
     6121 tttttgtctg aaacaatcac ttatctttct tcagcgcccc tagcgttggc gtttcgtcaa
     6181 agaagttcca tggtttaagc agagtatgta cccattcggt cggcataatc ggccactctt
     6241 cggcgcgggc cacatgtgtg gtgccggtgg tcatccagac aacggcgtcg gtgttgtcca
     6301 gcgactcgtt atctttactg tattgtccaa gaccggtgtc atgagtagaa cggttcggat
     6361 atttgccttc cgggaaacgc tcgccaggat gataacgcgt tacccaaagc tgcttgtcca
     6421 taaagcttaa acgatgatag atccactcgt ccggcgcgaa ctgggcacct tttgctaccg
     6481 ggtgagtacc acctgcataa ggaataattt gataggaaac tggattgccc atgcggttct
     6541 ctttgttcgg gttactcaac agacgaatcg tgcccggatc aaacttctgc gcggcatcct
     6601 gttcattgcc gatgttgtac tgattaactt gcatggtact ggtgcgtggg ccaccggcag
     6661 tattcggttt taccactggg tccatcgcca ccaggctgtt attctcgcca tctacatcca
     6721 gatcgaggcg gaaattataa atatgttggt gtgtagtacc cacgatattg tgatcgataa
     6781 gcgtgccgta gcgcgtgtca tctttcgccg tctcatcgtg catggttttc gctttaacac
     6841 ctttcaccgc ttcgatgccc gtagcaccgg catcaatgcc aatagtgccg ttttcatgga
     6901 agatccagtc aaaaatgtag tcatagttac ccactgtact gatccagcgc accactaact
     6961 cccggcgttc ggtactgacg ttgggctggc ccatttcctg atgcttatac tccggcccgg
     7021 cataacgttc aaataccgcg atagcgcgag ggatctccat cggcacgcca gtgtagtcgg
     7081 cgatggtttc attaaggagc actgcgttag acggggcatc tttaccacga gcaattggtg
     7141 aggttagcgt gcccataccg tagtcaccag agtccagata cgctttaaag taccagccaa
     7201 tatcaggatc accgtaaggc acaatcatgc cgccgagaga accttcgtac atgactttgc
     7261 gtttggtgcc attgtcgtta taagtcacgg tggagatcat cggcccgacg cgagagttca
     7321 tgctgaggtg aaaatcccag ttccgccagt gaatcatatc gccagtaatg gtgtaatttt
     7381 taccttcagg ctcaatgatt tgcataggct taactgccgg agcaacgcgg tcacggctat
     7441 caaatgggcg tgcggtcatt ggcaccggaa ctaccggacc ttcttcaatc ttaacgattt
     7501 ttttctgttc taaatcaacg accgccacca ggttttcgat gggatgtgcc cagtagttgc
     7561 catcaccgac atcaagatag ctgatgactt tgagcaaccg ggcatcttgt ttcaggccat
     7621 ctttaccatc gaaataacct acggtcagcg gcgtggtaat cacttttttc gcatcagtaa
     7681 taccgcgttt cttcacggca gcggcaaatt cttcactgtt gttaataatg ttctgcacac
     7741 tggcgaaatc atccagcaac accataccgt gggcgtcttt agtgggttgc caggagagca
     7801 gtttgttgtt ttgcagatcc accaccgctt cgatgatatg tttgccgtcg agcataatga
     7861 cgtcggcttt gcgcggctgg tcaaccggtt tgttttccag cgcaaacgcc cagacagctt
     7921 ctttatctgg cggtagcagg gagatctcag taaaacgggt attgggtttg aagtccgcgg
     7981 aagctttaac aatttcaacg gcctgtttaa tttcgtccgc agttagcgca ttaagtgggt
     8041 gagggcgctt ttctacctga aaggtttgat ccagcccgga ctggaaaaca tcgttaataa
     8101 aggtgtcaga aacccaggct ttattgtctt tcatcactac cggtacttgc agtgccagag
     8161 gctgaccatt aacaattgct gtttgcgcac caggcttcac tttcacgtac gcgccatctt
     8221 taatcagggt aaagagctgg gcgtagtcgt cccactgcac atcggcacca aattctttaa
     8281 gcgttttatc cattggcacc atatgcgctt caccaccgtg ggcaaatacc ggcgcttgcc
     8341 aggcgaaact taaggcgact gccaacgcca gggttgtttt acgggcagaa tacagagagg
     8401 ggcttcccat tattaacctc gtcagatgtt gtgttcttgt tagcaaccgc gctctgtggg
     8461 cggtttagtc aggttcacat tatcagtact gatgcaaagg ggattgcctg cacctgccag
     8521 gttgtttggc aggtgtgcca gcttttcata cagtggatgc cctgaaaata gatgtacaca
     8581 tcatgcataa tgtgacaacg tcacaaaatt tagtgaaata aaagggcaac tattcgccgt
     8641 tgcccttcat tcaccgatta atcgacaaaa tcaccgtgct gcctggccac cagcgtcaga
     8701 attgaataca gcgccaccgg ggtttgatgc tgattgaata cctctaccgc ccattccacc
     8761 acacctgttg gtttttcttc tgcgctacgc tgttttttca gcgtcttgcg cttacaggtg
     8821 agacgcacct ggatggtatc gcctggcttt acgggttcga taaaacgcaa gctttccagc
     8881 ccgtagttag caatgaccgg accaacaccg gcatcgacaa acagacccgc agccgcagaa
     8941 agcacaaaat acccatgcac cacccgctca ccgaaaatag attcggcagc agcaatctta
     9001 tccatatgtg catagaaatg atcgccgctg aggcaagcaa agttaacaat atcggcctct
     9061 gtcattgtgc ggcggggagt caacaggctg tcgcctggtt gtagctcctc aaaatatttg
     9121 cggaacggat gaatacgatc ttcttcgact ttcgcaccgc gcacccactg tttactgata
     9181 gcggcaagca tcgtcggact accctgaaca gcggttcgct gcatgtaatg tttcaccgct
     9241 cgtaaaccgc ctaattcttc accgcctcct gcgcgaccag gcccaccatg taccagttgt
     9301 ggcagtgggg agccatgccc ggtggattct tttgccgatt cttcattgag gatctgaatt
     9361 cgcccatgcg tacgtgccgc gtcggcaata aactgacgcg caatttgcgg atcagccgtc
     9421 accagcgttc ccgcaaggct accgccgcct gcacaagcca gttgcagagc atgttgctgg
     9481 ttttgtgctg gcattagcgt tgcgacaggg ccaaaggctt ctgttgcatg taccgccggt
     9541 gtttcatccg gctgcggaca gtacaataag gttggcggga agaaggcacc cgccgcagat
     9601 aaatccgcct gaccaccgag gcgaatctcg catcctgcag ccagcaatgt gttcactttt
     9661 tcctgcacat cagcacgctg ttcagcattt accagtgcgc ccattttcac gccttcttgt
     9721 gcaggatcac cgaccacgac tttctgtaat cgcgcaacca gagcatcact gacagcatta
     9781 accaatgtct gcggcacaat aatccgccgg attgccgtac atttttgccc ggcttttgtg
     9841 gtcatctcac gcacaacttc acgaataaac agcgcaaact ccggttgatc cggggtgata
     9901 tcttcgccca gtacgcagca attcagggaa tcagcttcca tggtgaaggg gatagatttg
     9961 gctacgatat ttggctgaac tcgtagcatc tgtccggtcg tcgcggaccc cgtgaaagtc
    10021 accacatcct ggctgtccag atgatccaaa aggtcgcccg cactaccgca gatcagacta
    10081 attgcgcctt cgggaacaag accactatcg acaattgatt tcaccatcgc ctgagtcagt
    10141 tgggccgtcg cggtagctgg tttgatgatg gctggcattc cgcccagcca cgttggtgcc
    10201 agcttttcca gcattcccca gcaggggaag ttaaaggcgt taatatgcac tgccacgcct
    10261 gactttgagg tcagtacatg gcgcgcggca aatccacctt ctttcgataa ggggagcaat
    10321 tcatcttccg gccacagcgt atcgtcaggc agctcccggc taccgaggct ggcgtaagta
    10381 aataacgtcc caatgccacc ttcaatatca acccaactgt ctgcccgcgt tgcgcctgtt
    10441 tgcgcagaaa gagcatagaa acgctctttt tcactcagca gatgtttagc gaccgcttta
    10501 agcatcgccg cacgttcgat aaaggtcata gcgcgaaggg ctggggcacc tttttcaatg
    10561 gcaaacaggc gggcagccgc catatcaaga ccttcactgg tcacttccca taacgcctcg
    10621 ccgctaatag cgtggtgaat caaacggcta cggccccggc cagactgcca ggtaccggat
    10681 aagaaactgg ctaactgctg catcgctact ctccagatgt ttcacatttc tgttgctaat
    10741 agttgaatcg cgaatcataa aaagcaaggg atcttttaat gaaatgttaa ctatgcgatc
    10801 tgtatagcaa ctgcggaaaa cattaatgca atgataaata atgatttata aaaagagggt
    10861 gcgaaatccg tcacagttca agcatacaaa atttgtgatt ttagttaacc attgtgtaac
    10921 tttcataaaa caatgtgatt cgtgttttta attaattcac gaaaactgga atcgtaaagg
    10981 tgatgacgtg acccaagaag aacgctttga gcaacggata gcccaggaaa cggctatcga
    11041 gccacaggac tggatgcccg atgcttaccg aaagacattg atccgccaga ttgggcagca
    11101 tgcgcactcc gagattgttg gtatgttgcc tgaaggtaac tggatcactc gcgcaccaac
    11161 cttgcggcgt aaagccattc tgttagccaa agtgcaggat gaagccggtc atggtctgta
    11221 tctctatagc gccgccgaaa cactgggctg tgccagagaa gacatctacc aaaaaatgct
    11281 cgacggacgg atgaaatatt cctccatctt taattatccg acattgagtt gggctgatat
    11341 cggcgttatc ggctggctgg tggatggcgc agcgatcgtc aatcaggtgg cgttatgccg
    11401 aacttcttat ggcccgtatg ccagagcaat ggtgaaaatc tgcaaagaag agagttttca
    11461 ccagcgtcag gggtttgaag cctgcatggc actggcgcag ggcagcgaag ctcaaaagca
    11521 gatgttgcaa gacgccatta accgtttctg gtggccagcc ttaatgatgt tcgggccaaa
    11581 cgatgataac tcgccaaaca gcgccagaag tctcgcctgg aaaatcaaac gtttcaccaa
    11641 cgacgaactc cgccagcgtt tcgtggacaa caccgttcca caggttgaaa tgctcggtat
    11701 gaccgttcct gacccggatc tgcattttga cactgaaagc ggtcactacc gctttggtga
    11761 gatcgactgg caggagttta acgaagtaat taacggtcgc ggaatttgta atcaggagcg
    11821 gcttgacgcc aaacgtaaag cctggaaaga aggtacctgg gtacgggaag cagcgctggc
    11881 ccatgcacaa aaacaacatg cccgtaaggt cgcataagga gattcaaaat gagtaatgtt
    11941 tactggccgt tatacgaagt tttcgtgcgt ggcaaacagg gcttatcaca ccgccatgtt
    12001 ggcagtttac atgctgccga tgagcggatg gcactggaaa atgcccgtga tgcttacacc
    12061 cgtcgtagcg aaggatgttc aatttgggtg gtgaaggcga gtgaaattgt tgcctcgcaa
    12121 ccggaagaac gcggtgaatt ttttgatccg gctgaaagca aggtctatcg ccatccaacg
    12181 ttttacacca tccctgatgg cattgagcac atgtgaggtc ggaaatgaat cagttaacgg
    12241 cttacacctt gcgcctgggc gataactgcc tggtgctctc ccagcggttg ggtgaatggt
    12301 gcggtcatgc accggaactg gaaatcgatc tcgcactggc aaatattgga ctcgatttat
    12361 taggccaggc acgcaacttc ttatcgtatg ccgctgaatt agcgggagaa ggcgatgaag
    12421 ataccctggc cttcacccga gacgagcgcc agttcagcaa cttattgttg gttgaacagc
    12481 caaacggcaa ttttgccgac accattgcac gccagtattt catcgatgca tggcatgtgg
    12541 cgctctttac ccgtctgatg gagagccgtg atccgcaact ggcggcgatt tctgccaaag
    12601 caattaaaga agcgcgttat cacctgcgtt ttagtcgtgg ctggctggag cgactgggca
    12661 atggtactga cgtatcaggg caaaagatgc agcaggccat caacaagttg tggcgtttta
    12721 ccgccgaact gttcgatgcc gacgagattg atattgcact gagtgaagag ggtattgcgg
    12781 ttgatccacg cactttacgc gcagcgtggg aagccgaagt ttttgccggg atcaacgaag
    12841 ccacattgaa cgtaccgcaa gagcaggcgt atcgcactgg cggtaaaaaa ggactgcata
    12901 ccgaacacct tggacccatg ctggcagaaa tgcagtatct ccagcgtgtc ttgcccggtc
    12961 agcaatggta acagaggaga tgggtatgca acgtctggcc accattgcac cgccacaggt
    13021 tcatgagata tgggcgctac tcagccagat cccggacccg gagatcccgg tgctgaccat
    13081 taccgattta ggcatggtgc gtaatgtgac acagatggga gaaggatggg tgatcggctt
    13141 tacgccgact tattccggtt gtccggcaac ggaacatttg attggcgcga tacgtgaggc
    13201 aatgacaacc cacgggttta cccccgttca ggttgtgctg caactcgacc cggcatggac
    13261 caccgactgg atgacccccg atgcccgtga gcgtctgcga cagtatggca ttagcccgcc
    13321 cgccggacac agttgccatg cccatttgcc gccagaagta cgttgcccgc gctgcgccag
    13381 cgtccatacc acacttatca gtgaatttgg ttccacggcc tgcaaagcat tgtatcgctg
    13441 cgatagttgc cgcgaacctt tcgattattt caaatgtatt tgaggatgcc atgacaacgt
    13501 ttcattcctt aacggtggca aaagtggagc cggaaacccg tgatgcggtg accattacct
    13561 ttgcggtgcc ccagcctttg caggaggcgt atcgctttcg ccccggtcaa catttgacct
    13621 taaaagccag ctttgatggt gaagaattac gccgttgtta ctccatttgc cgcagctatc
    13681 tgcctggcga aattagtgtg gcggtgaaag ccattgaagg cggacgtttc tcccgctatg
    13741 cccgcgaaca catccgccag ggtatgacgc tggaggtcat ggtgccgcag gggcatttcg
    13801 gctatcagcc acaggccgaa cgccagggcc gctatctggc tattgcagca ggatcaggta
    13861 ttacgccaat gctggcgatt atcgccacca ctttacaaac cgagcctgaa agtcagttca
    13921 ccctgatcta cggtaaccgt accagccaga gcatgatgtt tcgccaggca ctggcagacc
    13981 tgaaagacaa atatcctcag cgtttacagt tgttgtgcat tttcagtcag gaaaccctcg
    14041 acagcgatct gcttcacggg cgtattgacg gtgaaaaatt acagtcactt ggggcctcgc
    14101 tcattaattt tcgtctttat gatgaggcat ttatttgtgg tccggcggcg atgatggatg
    14161 agacggaagc cgccttaaaa gcactgggaa tgccagataa aaccattcat ctggagcggt
    14221 ttaatacgcc tggcacgcgc gtcaaacgta gcgttaacgt gcaaagtgac ggacaaaaag
    14281 tgactgtacg tcaggatggg cgggatcggg aaatcgtgct taatgccgac gatgaaagca
    14341 ttctcgatgc ggcattgcgc cagggggctg atctgcccta tgcctgcaaa ggcggcgtct
    14401 gtgcgacctg caaatgcaaa gtgctgcgtg gcaaagtggc gatggaaacc aattacagtc
    14461 tggaaccgga tgaactggcc gcaggctatg tgttgagttg ccaggcactg ccgctgacca
    14521 gcgatgtggt ggttgacttt gacgcgaagg ggatggcatg agcgaactga tcgtcagccg
    14581 tcagcaacaa gtattgttgc tgacccttaa ccgtcccgcc gcacgtaatg cgctaaataa
    14641 tgcccttctg acgcaactgg taaatgaact ggaagctgcg gctaccgata gcagcatttc
    14701 ggtctgtgtg attaccggta atgcacgctt ttttgccgct ggggccgatc tcaacgaaat
    14761 ggcagaaaaa gatctcgcgg ccaccttaaa cgatacacgc ccgcagctat gggcgcgatt
    14821 gcaggccttc aacaaacctc tcatcgcagc cgtcaacggt tacgcgcttg gtgcgggttg
    14881 cgaactggca ttgttgtgcg atgtggtggt tgccggagag aacgcgcgtt ttggtttgcc
    14941 ggaaatcact ctcggcatca tgccaggcgc aggaggaacg caacgtttaa tccgtagtgt
    15001 cggtaaatcg ttagccagca aaatggtgct gagcggagaa agtatcaccg ctcagcaagc
    15061 acagcaggcc gggctggtta gcgacgtctt ccccagcgat ttaaccctcg aatacgcctt
    15121 acagctggcg tcgaaaatgg cacgtcactc gccgctggcc ttacaagcgg caaagcaagc
    15181 gctgcgccag tcgcaggaag tggctttgca agccggactt gcccaggagc gacagttatt
    15241 caccttgctg gcggcaacag aagatcgtca tgaaggcatc tccgctttct tacaaaaacg
    15301 cacgcccgac tttaaaggac gctaatgatg gaattcatcc tcagtcatgt agaaaagggc
    15361 gtgatgacac taacgctcaa ccgcccggaa cgcctgaaca gttttaatga tgagatgcac
    15421 gcacaactgg cagagtgcct gaaacaggtc gagcgcgacg acactatccg ttgcctgtta
    15481 cttacaggtg ccgggcgcgg attttgtgct ggtcaggatc ttaacgatcg taacgttgat
    15541 cccacaggcc ccgcaccgga tttagggatg tcagttgaac gtttctataa cccactggta
    15601 cgtcgcctgg caaaactgcc aaaaccggtg atctgtgcag tcaatggcgt ggcggcaggg
    15661 gctggcgcaa cactggcact gggatgcgac atcgttattg ctgcccgttc agcaaaattc
    15721 gtcatggcgt ttagtaagtt aggtttagta cccgattgcg gtggaacctg gttactgcca
    15781 cgcgttgctg ggcgagcgcg cgccatgggg ctggcactgc tggggaatca actgagtgct
    15841 gaacaggcgc acgaatgggg gatgatctgg caggttgttg atgatgaaac gctggcagat
    15901 accgcgcaac agctggcacg gcatctggcg acacaaccga catttggtct tggacttatc
    15961 aagcaagcga taaatagcgc tgaaaccaat actctcgata cgcaactgga tctggaatgc
    16021 gactatcagc gacttgccgg acggagcgca gattatcgtg aaggtgtcag tgcgttcctg
    16081 gctaaacgct caccgcagtt cacggggaaa tagcatgatg ataaatgtgc aaactgtggc
    16141 agtgattggg agcggcacca tgggggctgg cattgctgaa gttgccgcca gtcatggaca
    16201 tcaggtttta ctgtatgaca tttctgctga agcgctgacc cgcgcaatcg acgggataca
    16261 cgcgcggcta aattcacgcg tgacgcgggg aaaactgact gctgaaacct gtgaacgcac
    16321 attgaaacgc ctgatcccgg tgaccgatat tcacgcgctg gcagctgcgg acctggtcat
    16381 tgaagcggcg tctgaacgtc tggaagtcaa aaaagcgctc tttgcacagc tggcggaagt
    16441 ttgcccgcca caaacgctat tgaccactaa cacttcgtca atctctataa ccgcgattgc
    16501 tgcggaggta aaaaatcctg aacgtgttgc ggggctgcat ttttttaacc cggcaccggt
    16561 gatgaagttg gtggaggtgg tcagtgggct ggcaacggca gcggaagttg ttgagcagtt
    16621 gtgtgaacta acgttgagtt ggggtaagca gcctgtgcgc tgtcattcga cccctggatt
    16681 tatcgttaac cgtgtggcgc gtccttatta ttccgaggcc tggcggacac tggaagagca
    16741 ggttgctgca ccagaagtga ttgacgctgc acttcgcgat ggcgctggtt tcccgatggg
    16801 gccgctggaa ttaaccgatc tgattggtca ggacgtcaat tttgctgtca cctgttcggt
    16861 gtttaacgct ttctggcagg agcgtcgttt tttaccttcg ctggtgcaac aggaactggt
    16921 gattggtgga cggttgggca agaaaagtgg gctgggcgtg tacgactggc gcgcggaacg
    16981 tgaggcagtt gttggcctgg aagcggtaag cgacagtttt agcccaatga aagtagaaaa
    17041 gaaaagtgac ggtgtcacgg aaattgacga tgttttattg attgagacac aaggcgagac
    17101 ggcacaggcg ctggcaatac gactggcacg cccggtggta gtggtcgaca aaatggcggg
    17161 caaggtggtg accattgctg cggcagcggt gaacccggac tcagcgaccc gcaaggccat
    17221 ttattacctg caacagcagg gcaaaacagt gctgcaaatt gcagattacc caggaatgct
    17281 gatttggcga acggtagcaa tgatcatcaa tgaagccctt gatgcgctgc aaaaaggcgt
    17341 ggcctctgaa caggatatcg ataccgccat gcgtcttggg gtgaattatc catatggccc
    17401 gcttgcctgg ggagcgcaac ttggctggca gcgaatatta aggctcctgg aaaatctaca
    17461 acatcactat ggcgaagaac gctatcgccc atgttcattg ctgcgccaac gagcgcttct
    17521 ggagagcggt tatgagtcat aaggcctggc aaaatgccca tgcaatgtat gagaacgatg
    17581 cctgcgccaa agcgcttggc atcgacatta tctcaatgga tgaaggcttt gctgtagtga
    17641 ccatgaccgt cactgcacaa atgcttaatg gtcatcaaag ttgccacggc gggcagctat
    17701 tttcactggc tgatactgcc tttgcctacg cttgcaatag ccaggggttg gcagctgtcg
    17761 cttctgcctg cacgattgat tttttacgtc caggctttgc cggagacacc ttaactgcga
    17821 ctgcgcaggt acgtcatcag ggcaagcaaa ccggtgttta cgacatcgaa attgttaacc
    17881 aacaacaaaa aacggttgcg ctgtttcgcg gtaaatctca ccgcatcggc ggcaccatta
    17941 caggagaagc ctgatgcgtg aagcctttat ctgtgacgga attcgtacgc caattggtcg
    18001 ctacggcggg gcattatcag gtgttcgggc tgacgatctg gctgctatcc ctttgcggga
    18061 gctgctggtg cgaaacccgc gtctcgatgc ggagtgtatc gatgatgtga tcctcggctg
    18121 tgctaatcag gcgggagaag acaaccgtaa cgtagcccgg atggcgactt tactggcggg
    18181 gctgccgcag agtgtttccg gcacaaccat taaccgcttg tgtggttccg ggctggacgc
    18241 actggggttc gccgcacggg cgattaaagc gggcgatggc gatttgctga tcgccggtgg
    18301 cgtggagtca atgtctcggg caccgtttgt tatgggcaag gcaaccagtg cattttctcg
    18361 tcaggctgag atgtttgata ccactattgg ctggcgattt gtgaacccgc tcatggctca
    18421 acaatttgga actgacagca tgccggaaac ggcagagaat gtagctgaac tgttaaaaat
    18481 ctcgcgagaa gatcaagata gttttgcgct acgcagtcag caacgtacgg caaaagcgca
    18541 atcctcaggc attctggctg aggagattgt tccggttgtg ttgaaaaaca agaaaggcgt
    18601 tgtaacagaa atacaacatg atgagcatct gcgcccggaa acgacgctgg aacagttacg
    18661 tgggttaaaa gcaccatttc gtgccaatgg ggtgattacc gcaggcaatg cctccggggt
    18721 gaatgacgga gccgctgcgt tgattattgc cagtgaacag atggcagcag cgcaaggact
    18781 gacgccgcga gcgcgtatcg tagccatggc aaccgccggg gtggaaccgc gcctgatggg
    18841 gcttggtccg gtgcctgcaa ctcgccgggt gctggaacgt gcggggctga gtattcacga
    18901 tatggacgtg attgaactga acgaagcgtt cgcggcccag gcgttgggtg tactacgcga
    18961 attggggctg cctgatgatg ccccacatgt taaccccaac ggaggcgcta tcgccttagg
    19021 ccatccgttg ggaatgagtg gtgcccgcct ggcactggct gccagccatg agctgcatcg
    19081 gcgtaacggt cgttacgcat tatgcaccat gtgcatcggt gtcggtcagg gcatcgccat
    19141 gattctggag cgtgtttgag catatcaacc tgcgagtacc ctacaatgat aaccaataca
    19201 aagcttgacc cgattgaaac cgcgtctgtt gatgagttac aggcgttgca aacacagcgt
    19261 ctgaaatgga cgctcaaaca cgcgtatgaa aatgtcccga tgtatcggcg caaattcgac
    19321 gcagcaggcg tacatcctga tgatttcagg gaactttcag acctgcgtaa atttccctgt
    19381 accaccaaac aggatctgcg cgacaactat ccctttgaca cctttgccgt gccgatggaa
    19441 caagtggtgc gcattcatgc ttcttcggga accacaggta aaccgacagt tgtcggttat
    19501 acgcaaaacg atattgataa ctgggccaat attgtagcgc gttctttgcg tgcggcaggg
    19561 ggctcgccca aagacaaaat tcatgttgcc tatggttacg gcctgtttac tggtgggctg
    19621 ggcgcgcact atggtgccga acgtctgggc gctacggtga tcccgatgtc tggcgggcag
    19681 acggaaaaac aagcgcaact gatccgcgat tttcaaccag atatgatcat ggttacgcca
    19741 tcttattgcc ttaacctgat tgaagagctg gagcggcagt tgggcggtga tgccagcggt
    19801 tgctcgctgc gggttggcgt gtttggtgct gagccgtgga cacaggccat gcgtaaagag
    19861 attgaacggc gtctggggat caccgcactg gatatttatg gcctgtcaga agtgatgggg
    19921 ccgggggtgg cgatggagtg tctggaaact accgacggtc caactatttg ggaagatcat
    19981 ttctatcctg aaattgttaa tcctcatgac ggcacaccgc ttgccgatgg tgaacatggc
    20041 gaactgttat tcaccacgct gaccaaagaa gcattgccgg tcattcgtta ccgcacgcgt
    20101 gatttaaccc gactgttacc aggaacggcg cggactatgc gccgcatgga tcgcatcagc
    20161 ggacgcagcg atgacatgtt gattattcgc ggtgtaaatg tctttccgtc acaactggaa
    20221 gaagagattg tcaaattcga acatttatcg ccgcattacc aactggaggt gaaccgccgg
    20281 gggcatcttg attcactttc tgtgaaagtg gagttgaaag aaagtagcct gacattgacg
    20341 catgagcagc gttgccaggt atgccaccag ctgcgccatc ggattaagtc gatggtgggg
    20401 atctctaccg atgtgatgat cgttaactgt ggcagtatcc cgcgttcaga aggcaaggcg
    20461 tgtcgggtgt ttgatctgcg caatattgtt ggtgcctgac gtgtcgtctg gccctggtga
    20521 ggtaaagcgc cagggccaga agtcgatacg acctgtgcta tgattcataa atcacaacaa
    20581 taacaacaga ctgaatcgaa tgagtaaact tgatactttt atccaacatg ctgtaaacgc
    20641 tgttcctgtc agtggcacat ctttgatctc ctctctgtat ggtgattctc tttcccatcg
    20701 tggtggtgaa atctggctgg gtagtctggc tgctttgctg gaagggctgg gatttggtga
    20761 gcgtttcgtg cgcactgctt tgtttcgtct taataaagaa ggctggctgg atgtttcccg
    20821 catcgggcga cgcagtttct atagcctcag tgataaaggc ttgcgcctga cgcgacgggc
    20881 agaaagtaaa atttatcgcg cagagcaacc cgcatgggat ggtaaatggc tcctgttgct
    20941 ctcggaaggt ttagataaag cgacgctggc tgatgtcaaa aagcagttga tctggcaagg
    21001 ttttggtgca ctggcgccca gcctgatggc atcgccgtcg caaaaactgg ccgatgtaca
    21061 gacacttttg catgaagcgg gtgtggcgga taacgtgatt tgttttgaag cgcaaatacc
    21121 actggcgctt tctcgtgcag cactgcgtgc cagagtagaa gagtgctggc atttaactga
    21181 acaaaatgcc atgtacgaaa cctttattca gtcattccgc ccgttggtgc agctgttaaa
    21241 agaggcagca gacgagttaa ccccggagcg tgcatttcat attcagcttt tactgatcca
    21301 tttttatcgc cgtgtcgtcc ttaaagaccc tttgttgccg gaggagttgc ttccggctca
    21361 ctgggcaggg catacggcgc gtcagctgtg tatcaacatt tatcagcgcg tagcgcctgc
    21421 tgctttagcg ttcgttagtg aaaaaggtga aacctcggtc ggtgaactgc cttcgccggg
    21481 tagcctgtat tttcaacgtt ttggcggttt gaatattgaa caggaggcgt tatgccaatt
    21541 tatcagatag acggtctgac tccggttgtg ccagaagaga gttttgtcca tccgacagcg
    21601 gtattgatcg gcgatgttat tctcggcaag ggtgtttacg ttgggccaaa tgccagcctg
    21661 cgtggcgatt ttggtcgtat cgtggtgaaa gatggcgcga acattcagga taattgcgtt
    21721 atgcacggtt ttcccgagca ggatactgtc gttgaagaag atgggcatat gggtcatagc
    21781 gctatccttc acggctgcat tatccgccgc aatgcattag tgggaatgaa cgcggtagtg
    21841 atggatggtg cggtgattgg cgagaacagc attgttggcg catccgcatt tgtgaaagcc
    21901 aaagcagaaa tgcctgccaa ttacctgatt gtcggcagcc cggcgaaact gattcgtgag
    21961 cttagtgagc aggagctggc atggaaaaag cagggtacgc atgagtatca ggtgctggtg
    22021 acacgctgta agcagacgtt acatcaagtc gaaccattgc gggaagttga acctggcagg
    22081 aaacgcctgg tatttgatga gaatctgcga ccgaaacagt aacgtccaga tactgaacgt
    22141 tctaattttt agcatgtgat acctaaaccg caacgctgat acaggttgcg gtttttttat
    22201 tgccgaatgc ggcgctttac gcgttttgtt ttggtgtctt tcaggacaat agagcaactc
    22261 atccagtaat cttgtttacg ccttcgcatt atttatctct tttcgtttct atactgattt
    22321 ttctcaatcc gttttattac agggcagggt gcgatgagca gcaatacatt tactctcggt
    22381 acaaaatccg ttaaccgtct tggttatggc gcgatgcaac tggcaggtcc tggggttttt
    22441 ggtcccccaa gagatcgcca cgtcgctata accgtgctgc gcgaggcgct ggcattgggc
    22501 gtcaatcaca ttgataccag cgacttttat ggtccgcacg tcaccaatca gattatccgc
    22561 gaagcgcttt atccttactc tgacgacctg acaattgtca ctaaaattgg tgcgcggcgt
    22621 ggagaggacg catcctggtt gcccgcattt tctccggcag agctgcaaaa agcggtgcac
    22681 gataatctac gtaatctcgg gctggacgta ctggatgtgg ttaacttgcg cattatgatg
    22741 ggggatggtc atggcccagc ggaaggatcg attgaagcca gcctgaccgt gctggcagag
    22801 atgcaacaac aaggcctggt aaaacatatt ggcctaagca acgtcacacc gacgcaggtt
    22861 gcagaggcgc gcaagattgc cgaaatcgtc tgtgtgcaga acgaatacaa cattgctcat
    22921 cgtgctgatg acgcaatgat tgatgctttg gctcgtgacg gcattgccta cgtgccgttc
    22981 ttcccgctcg ggggctttac accgctgcaa tcctctacgc tgtcggatgt tgctgcgagc
    23041 ctgggtgcaa cacccatgca ggtggcgctg gcgtggctgt tacaacgttc accgaatatt
    23101 ttgctgatcc cagggacgtc ttctgttgcg catttacggg agaatatggc tgctgaaaaa
    23161 ttgcatcttt ctgaggaagt gttgtctacg ttggatggta tttcgcgaga ataacgaata
    23221 tacaaaaggg aaagatgcat ttcccttttg tttctttttt aatggcatgg agtgcatatg
    23281 tttaaggcca ggatttgcgg ctggataggt ttacttcccc tgtttatgct gagtcttcca
    23341 gtacaggcgg aacttcgatg tgttgcaaat acagttgata ttgaaccatt tttttctgcg
    23401 gctaccgctg aagataaaca acaagttgaa caagctatca acagtagcgt gaatcttgtc
    23461 cccttcggtt tatctgcatc ggactggaaa gtgcatcgtg gcgatttagt ggtagaaggt
    23521 aatatagaga gtaatcaaaa attgattgtt cttggtaatt tgacagtcaa aggtaatatt
    23581 tccactttct ctctttctaa tccatgggtt attctcggta acgtgacggc aacgaatata
    23641 gttacggatt caccactatt aattaccggt tcgataaatg caagtggact ggtatttatc
    23701 gactcatatt acgataatcc gtcttcgatt aaggggagta ttaatgcgcg tggaatattt
    23761 atcaatgaca taattgcacc tgttgttgcg tcttcgacaa atagtgaatt catggtccgt
    23821 gcgagtgaca aaaatgacac tgaaaatgtc aaaaaagcgc tgatgataat aaatcctgat
    23881 gcatattatt ggggtctaat aaatgatgaa gatgctctga aagaaatttt taagcgaagc
    23941 aatattcgca tggcagggaa tgtctgtaat cagatgaaaa aagaagcgct gtttcgccct
    24001 aagccttctc ctgagttagt gcaggaattg caaatgctgg atgaaggcaa cgttgctgca
    24061 tttgaaggac gagatattgc gacatttgat cttgccataa tgcgaactct cccaaggctt
    24121 aaaggaattt cagctaacct acgcaaacaa cttattaata gcaatgatga acaaacgatt
    24181 gaaagtatgg cgagatatat gcctgacaat gagattctgg agttgaccga tcagcaattg
    24241 ggttaccagc ctgttgttct ggggttgctc gaccgtgaac cgctctctgt cgaaataatg
    24301 acgcgaatga gtcgtctacc tgatggtgtc ggtccgttga atcttgcgct acgtgaaaat
    24361 ctccctctgg atatcgtaat gactctggcg aaaagagatt gggatatgat cattcaggag
    24421 ctttataaag atgcgtggtt attgcctgaa tccattattg atggctatat ccgcagtgat
    24481 gattcctcta ttcgtcaggt cggtgctgga ggacaactca cctacaatca ggcaatgcag
    24541 ttggcgaacg attcatcaaa caatgttgtc acaagcttag cgttcaagct ggcagagatg
    24601 aaacaccatg gtcaattatt gcggatgacg ccacaagaga gtgacaaagt tgcagcctat
    24661 ttataccaaa aattcgagaa tgacgatgat ctaatacgtg tgttattttt agcattgcca
    24721 gataacttac agtttaattt tgttaaaagg atggagaaaa aatcccctgc ctacttttgc
    24781 tgtcgggata tgcaggtaat tcactctgac gctgctctac aacggttatt gacacgtttc
    24841 aacgatcctg aggggtggag taatctggcg aaaaatcagt atctgagcac gtcgatgaaa
    24901 caaaaaattt ggcaacgtgc tttatcgcat cggaaaaata atccgaaagc agattcagct
    24961 gcgtatgaaa ccagcgctga tatgattttg tctgagctta tcagccacgg tgaagttgat
    25021 gatcagatgc tattaaatgc caccgccctg atacgtttgg aagactggga ctttttagag
    25081 agtgcattag ttagttggga taatttaccc gctgttgtcc tcaaggaatt acagcaaaat
    25141 acgccacgca atgatatttg ggcgaagttt tttctgaggc aggaaaacag ctcccgtgcg
    25201 caggttgatg aagcattacg cgtctattat gcactcgacc ccgatgcgtt agcacaactg
    25261 gatgtactgg caaaacaacc ggatcgtata tggtggagta cactggcgaa aagcaatctc
    25321 acatttttca agttcggcgc acttaacaac cgccacacac cgcctgcagt actggcggca
    25381 gaaattgatc ccgagtggtg gattgtggcg atgaataatc cccgttttcc agttgatgta
    25441 ttaaaggcga ggctgaaacg tgatcctttg ctggcgttag agcttgttaa ccctgaactg
    25501 gatttagtcc gccagctggc gcttaacggt aagacgcgcg caatacggga acaagcgatg
    25561 agaaaacttg atgagttgta ttgatccaca acgtaatatt tcttaaccct ttgattaatt
    25621 ggggataatt attcctgaca gtgagtcccc aataccttga tatattctga atttttaatg
    25681 aagcggcgtg ttgcgatatc tccgtcaggg gaattgatgc accatagcgc aaaccgaatt
    25741 atcaaggatt gataatgacg ctctaccaga taaaaccgct ctttcagtcg ctgttaaggc
    25801 cgacgatgtt ttggctttac aagcaccacg ttacagcgaa tcacatcact cttgctgcac
    25861 tggcgctttc tcttcttacc ggattgctgt tgatgttggt ggcacaaccc atcctcttta
    25921 tgctattgcc catcgtgctt tttatccgta tggcgctcaa tgcgctggat ggcatgttgg
    25981 cgcgtgagtg caatcagcaa acacggcttg gggcaatttt gaatgagact ggtgatgtta
    26041 tttccgatat tgcgctctat ttaccatttt tatttttacc ggaaagtaac gcatcactcg
    26101 tgatactcat gttattttgc accatattga ccgagttttg cggtttactc gcgcagacga
    26161 ttaacggtgt tcgcagttat gccggtccat ttggcaaaag cgatcgcgcg ttaatatttg
    26221 gtctgtgggg tctggccgtt gccatttatc cacagtggat gcagtggaat aatcttttat
    26281 ggagcattgc ttcaattctg cttctctgga ctgcgattaa tcgttgtcgg agtgtgcttc
    26341 ttatgagcgc tgaaagataa tgctggaaaa atctctggca acacttttcg cgttgttaat
    26401 tttagcaacg ctgataaacc attttctggt atggcggtta ccggagagaa aagaggatga
    26461 agttacatta cgtattcgta cctggtgggg catcgtcatt tgtttttcac tggtgatttc
    26521 aggtccacgc tggatgacgt tgacgttttt tgcgctgata agttttctgg cattgaaaga
    26581 atattgtacg cttatatctg tacattttcc gcggtggtta tattgggtta ttcctcttaa
    26641 ctatttgttt atcggtttta attgctttga gctgtatcta ttatttatac ctttggctgg
    26701 gtttctaata ttagccaccg ggcaagtttt tgtgggggac ccctccggtt ttctgcatac
    26761 cgtgagcgcc attttttggg gctggataat gaccgttttc gccttgagtc atgccgcctg
    26821 gttattaatg ttgccaacca caaatatcca gggcggggcg ttactggtgt tatttctttt
    26881 ggcattaacc gagtcaaacg atattgcaca gtatttatgg ggaaaatcct gcggcagaag
    26941 aaaagtggtc cccaaagtca gcccgggaaa aacgctggaa ggtctggtgg ggggcgtcat
    27001 caccaccatg attgcgtcac tgattattgg accgttgctg acaccgctaa atacattaca
    27061 ggcattatta gcgggtttgt taattggtat tagtggtttt tgcggcgatg tcgtgatgtc
    27121 agccatcaaa cgagatattg gtgttaaaga tagtggaaaa ctattaccag gacatggcgg
    27181 actccttgac aggattgact cattaatttt caccgctccg gtattttttt attttattcg
    27241 ctacagctgt tactgaagga aaatagagaa aatggaaaat tcacgcatcc ctggggaaca
    27301 tttttttacc accagtgata atacagcgtt gttttatcgg cactggcccg ctttacagcc
    27361 cggggcgaaa aaggtcatcg tcttatttca tcggggtcat gaacattctg gtcgtctaca
    27421 acatctcgtt gatgaactgg cgatgccaga tactgctttt tatgcatggg atgcccgggg
    27481 gcatggaaaa acttcggggc cgcgtggtta tagcccatct cttgcgcgtt cagtgcagga
    27541 tgtcgatgaa tttgtccgtt ttgctgccag cgacagccag gttggactgg aagaggtggt
    27601 tgtgatcgcg caaagcgtcg gcgcagtgct ggttgccaca tgggttcatg attatgcgcc
    27661 tgcaattcgc gggctggtgc tggcttctcc ggcctttaag gttaaattgt atgtgccgct
    27721 ggcacgtcct gcgctggcgt tatggcatcg tctgcgtggt ctgtttttta ttaattccta
    27781 tgtgaaagga cgctatttga cccacgatcg gcaacgggtg gcgagtttca ataatgatcc
    27841 gctgatcaca cgggcgattg ccgtcaatat cttgctcgat ctctacaaaa cgtctgaacg
    27901 tattattaga gatgcggcgg cgattacgct ccccacgcaa cttctgatat caggcgatga
    27961 ctatgtggtg catcgccaac cgcagattga tttttatcag agattacgta gccctctgaa
    28021 agagctgcat ctgctgccag gcttttatca cgacacgttg ggtgaagaga acagggcgca
    28081 ggcatttgaa aaaatgcaaa gctttattag tcgtttatat gctaacaagt cacaaaaatt
    28141 tgattatcag catgaagacc gcactggacc atcagcggat cgctggcggc tcctttcagg
    28201 tggacccgtg ccattatcgc cggttgattt ggcgtatcgc tttatgcgaa aggcgatgaa
    28261 attgttcggg gcgcactctg cgggcctgca tctcggaatg agcaccggct ttgattcagg
    28321 cagttccctg gattatgtct atcaaaatca accgcaaggc agtaacgcat tcgggcgctt
    28381 aattgacaaa atctacctga acagtgttgg ctggcgcggt attcgccagc gtaaaaccca
    28441 tttacaaata ctgattaaac aagccgttgc cgatctccac gccaaaggtt tagccgtccg
    28501 cgtggttgac attgccgcag ggcatgggcg ctatgtactg gatgcgctgg caaacgagcc
    28561 tgccgtaagc gatattttgt tacgtgatta cagtgagtta aatgttgcac aggggcaagc
    28621 gatgattgcc caacggggaa tgtctgggcg ggtgcgtttt gaacagggcg atgcgtttaa
    28681 cccggaggaa ctcagcgcgt taactccgcg gcctacgctg gcgattgtct ctggcctgta
    28741 tgagcttttt cccgaaaatg agcaggtaaa aaactcactc gcaggtcttg ccaatgccat
    28801 cgaaccgggc ggcattctca tctacaccgg gcagccgtgg caccctcaac tggagatgat
    28861 tgccggggtg ttaaccagtc ataaagatgg taaaccgtgg gtaatgcgcg tgcgttcgca
    28921 aggggagatg gattcactcg tgcgtgatgc cggatttgat aaatgcacac aacggattga
    28981 tgagtggggt atttttacgg tttcgatggc ggtgcgtcgt gataactgaa cgtcggaacg
    29041 tattgctaca aggcgctggc tggttattgt tgctggcccc gtttttcttc ttcacctatg
    29101 gatctcttaa tcagttcacc gcggttcagg accttaacag ccatgatatc cccagtcagg
    29161 tattcggttg ggaaacggcg atcccttttc ttccctggac tattgttcct tactggagtc
    29221 tggatctttt atatggattt tcgctgttcg tttgtagcac gacattcgaa cagcgccgac
    29281 ttgtccaccg gcttattctg gcaacggtaa tggcctgctg cggttttttg ctctatccgc
    29341 tgaagtttag ttttatccgt cctgaagtga gtggggtgac gggatggcta ttttcgcaac
    29401 ttgaactgtt tgatctgcct tataaccagt ctccttcgct gcatattatt ctctgctggc
    29461 tactttggcg tcactttcgt cagcatctgg ctgagaggtg gcgtaaagtc tgcggcggat
    29521 ggtttttact catcgctatt tctactctga cgacctggca gcatcatttt attgatgtca
    29581 tcacagggct ggcggtaggt atgttgattg actggatggt gcccgtcgac cgtcgttgga
    29641 attatcagaa acctgatcaa cgtcgaatca aaatagcact gccctatgtc gtaggcgcgg
    29701 gctcgtgcat tgtattgatg gagctaatga tgatgattca gttatggtgg tcagtctggt
    29761 tatgttggcc agtattatcg ctactcataa ttggccgtgg gtacggtggg cttggcgcga
    29821 taacaacagg gaaagatagt caggggaaac taccgcccgc cgtttactgg ctgacattgc
    29881 cctgccgcat cgggatgtgg ctgtctatgc gttggttttg tcgtcgcctg gagccggtga
    29941 gcaaaatgac tgctggtgtt tatttagggg cgtttccacg acatattccg gcacagaatg
    30001 cggttctgga cgtcaccttt gaattccctc gcggacgagc gacaaaagat cgactctatt
    30061 tctgtgtacc gatgctggat ctggtggttc cggaagaggg ggagctccga caggccgtgg
    30121 cgatgctgga aacattacgc gaagagcaag gcagcgttct ggtccattgc gcgttgggat
    30181 tatcgcgcag tgcgctggta gtggcggcat ggttgttatg ttacggacac tgtaaaaccg
    30241 ttgatgaagc gattagttat attcgagcca gacgttcgca tattgtgctt aaggaagatc
    30301 acaaagcgat gctgaaatta tgggaaaaca ggtaagtgga ttgagatgtg gactgaacta
    30361 cagtccacat caagatcgtg tccggttatg cagcaacaat gctgtcgatg gccgctttcg
    30421 cgtcagactg cgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa
    30481 atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt
    30541 ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct tttttacccg
    30601 ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg cgtgcaacca
    30661 ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt gccgcaataa
    30721 cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct
    30781 gctgacgcgg agtcagcggc gcatcgctcg gacgcagtgc accaaccagt tcgccatcca
    30841 gtaccggaat cggatttgca gccaggtcgc gaacggtgat ttcatcagcg gagtgctttt
    30901 cgcgccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga
    30961 tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt ttgaatgggc
    31021 gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagata gcgcaataaa
    31081 tcgaagccta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat
    31141 atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt tatgacagaa
    31201 caacaaaaat tgacctttac ggccttgcag cagcggctgg attcgctgat gctgcgcgac
    31261 agactgcgtt tttctcggcg tctgcacggt gttaagaagg ttaaaaatcc tgatgcacaa
    31321 caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa agtcctgctg
    31381 cgtgaagcgg cacgaccgga aattacttat cctgacaatt taccggttag tcagaaaaaa
    31441 caggacattc tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaacgggt
    31501 tctggtaaaa cgactcagtt accgaaaatc tgtatggagc tggggcgcgg gattaaaggg
    31561 ctgatcggcc atacccagcc gcgtcgcctg gcggcaagaa cggtggcgaa ccgtattgcg
    31621 gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac
    31681 gttagtgata acacgatggt caagctgatg accgacggta tcctgctggc ggagatccag
    31741 caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcaca cgaacgcagc
    31801 ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta
    31861 aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat
    31921 gcgccgatta tcgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgaccgatt
    31981 gttgaagaag ccgacgacac cgagcgcgat cagttgcagg cgatttttga cgccgtagac
    32041 gaactgagtc aggaaagccc tggcgacatt ctgatcttta tgagtggtga gcgggaaatt
    32101 cgcgataccg ccgatgcgct gaacaagcta aatctgcgtc ataccgaaat cttgccgctt
    32161 tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag cggacggcgc
    32221 attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat taaatacgtt
    32281 atcgaccccg gtacagcacg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg
    32341 ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg tgggcgtgtg
    32401 tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctttcgcg cccggagttt
    32461 accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg
    32521 gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag
    32581 gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc
    32641 tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgatcc acgtctggcg
    32701 cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc
    32761 gcgctttcca ttcaggatcc gcgcgagcgt ccgatggata aacagcaggc atcggacgaa
    32821 aaacatcgtc gcttccacga caaagagtct gacttcctcg cgtttgtgaa tctgtggaat
    32881 tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc
    32941 gattatctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag
    33001 gtggtgaaag aacttggcat tccggttaac agcgaaccgg cggagtatcg cgaaattcac
    33061 attgcgttgc tgacgggttt actttcccat atcggcatga aagatgccga taaacaagaa
    33121 tataccggcg cacgtaacgc gcgtttctcc atctttcccg gttctggttt attcaaaaaa
    33181 ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt
    33241 gctgcgcgta tcgacccgga atgggtggag cctgtcgctc agcatttgat taaacgcacc
    33301 tacagcgaac ctcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact
    33361 gtttatggtt tgccgattgt tgccgcgcgc aaggtcaact acagccagat cgatccggcg
    33421 ttatgccgtg aactctttat tcgccatgcg ctggtggaag gtgactggca gacgcgtcac
    33481 gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca
    33541 cgtcgccgcg atattctggt tgatgacgaa acgttgtttg agttctatga ccagcgcatc
    33601 agccacgatg tgatctctgc acgccacttc gacagctggt ggaaaaaagt cagccgcgaa
    33661 acccctgatt tgctcaactt tgaaaaaagc atgttgatca aagagggcgc agaaaaaatc
    33721 agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat
    33781 cagtttgagc ccggcgcgga tgctgacggt gtgaccgtac atattccgct gccgttactt
    33841 aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaactggtg
    33901 attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtacc cgcaccaaac
    33961 tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct cgacagcctt
    34021 gagcgcgagt tacggcggat gaccggtgtt accgttgacc gcgaagactg gcactgggat
    34081 caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg acgacaaaaa taagaagcta
    34141 aaagaagggc gctcattaca ggatctgaaa gatgcgctga aaggcaaagt gcaggaaacg
    34201 ctgtccgcgg tggcggatga cggtatcgag cagagcgggc tgcatatctg gagctttggg
    34261 caactgccgg aaagctacga acagaagcgc ggcaactata aagtgaaggc gtggccggcg
    34321 ctggtggatg aacgtgacag tgtggcgatc aaactgtttg ataacccgct ggagcaaaaa
    34381 caggcaatgt ggaacggtct tcgccgtcta ctgctgctaa atattccctc gccgatcaag
    34441 tatttacatg agaagttacc aaacaaagcc aagcttggac tgtactttaa cccgtatggc
    34501 aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact gatcgacgcc
    34561 aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgagaa agtgcgtgcc
    34621 gaactgaacg acacggtggt agatattgcg aagcaggtcg agcaaattct tacggcagtg
    34681 ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact ggggctttct
    34741 gacattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg taacggcttc
    34801 aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacggct ggaaaaactg
    34861 gcggttgatc cacatcgcga ccgtgctcag atgctgaaag ttgaaaacgt ccagcaggcg
    34921 tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga cgtgaaagag
    34981 atccgttgga tgatagaaga gttgcgcgtt agttacttcg ctcaacaact tggtacgcct
    35041 tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg ttaaccctgc
    35101 tatttgcctg ataaagaaaa aacccggtaa gcatttagcg ccgggttttt tattaattct
    35161 aaaacggtaa gggtaaaaat tcaggaattc agaaaaatac aattctctgc tgcaagatga
    35221 ataatgttta tctacagcat ttccttaaaa gatatgtcag gcttgcggag tggcggttaa
    35281 ggacatacga cttcctcctt tcagagtgct ccgcttctca ctattatctc acacagtatt
    35341 cttaagggaa cgataaggag gaaccatgaa cattaccccg tttccgacgc tttcgccggc
    35401 aactatagat gccataaatg ttatcggaca gtggctggcg caggatgatt tctccggtga
    35461 ggtgccgtat caggccgatt gcgtgatcct tgcaggcaat gcggttatgc cgactatcga
    35521 cgcggcatgt aagattgctc gcgatcagca aattccttta ctgattagtg gtggtatcgg
    35581 tcactcgaca acttttttgt atagcgccat cgcacagcat ccgcactaca acactatccg
    35641 caccactggc agagcagaag cgaccatcct ggcggatatc gctcatcagt tctggcacat
    35701 tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac tgcggtgaaa acgcacgctt
    35761 tagcatcgcg ctattgaaac aggccgtaga acgagttcat acggctatcg ttgttcagga
    35821 ccccaccatg cagcggcgca cgatggcgac gttccgccgt atgactgggg acaatcccga
    35881 tgcaccacgc tggttaagtt atcccggatt cgttcctcag ttaggaaata acgcagacag
    35941 tgtaatcttt attaatcagt tacaaggatt atggccggtt gagcgttatc tctcactact
    36001 cactggcgag ctgccgcgtt tacgcgatga tagcgatggc tacggtcccc gcgggcgaga
    36061 ttttatcgtt cacgttgatt ttccggcaga agtcatccat gcatggcaaa cgctgaaaca
    36121 tgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta cgttaaaaat tgcccgtttg
    36181 tgaaccactt gtttgcaaac gggcatgact cctgactttt atttctgcct tttattcctt
    36241 ttacacttgt ttttatgaag cccttcacag aattgtcctt tcacgatgcc gtctctctga
    36301 tgattgatgt taattaacaa tgcattcacc gaaaataaac atataaatca caggagtcgc
    36361 ccatgtcagt acccgttcaa catcctatgt atatcgatgg acagtttgtt acctggcgtg
    36421 gagacgcatg gattgatgtg gtaaaccctg ctacagaggc tgtcatttcc cgcatacccg
    36481 atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc agaacgtgca caaccagaat
    36541 gggaagcgtt gcctgctatt gaacgcgcca gttggttgcg caaaatctcc gccgggatcc
    36601 gcgaacgcgc cagtgaaatc agtgcgctga ttgttgaaga agggggcaag atccagcagc
    36661 tggctgaagt cgaagtggct tttactgccg actatatcga ttacatggcg gagtgggcac
    36721 ggcgttacga gggcgagatt attcaaagcg atcgtccagg agaaaatatt cttttgttta
    36781 aacgtgcgct tggtgtgact accggcattc tgccgtggaa cttcccgttc ttcctcattg
    36841 cccgcaaaat ggctcccgct cttttgaccg gtaataccat cgtcattaaa cctagtgaat
    36901 ttacgccaaa caatgcgatt gcattcgcca aaatcgtcga tgaaataggc cttccgcgcg
    36961 gcgtgtttaa ccttgtactg gggcgtggtg aaaccgttgg gcaagaactg gcgggtaacc
    37021 caaaggtcgc aatggtcagt atgacaggca gcgtctctgc aggtgagaag atcatggcga
    37081 ctgcggcgaa aaacatcacc aaagtgtgtc tggaattggg gggtaaagca ccagctatcg
    37141 taatggacga tgccgatctt gaactggcag tcaaagccat cgttgattca cgcgtcatta
    37201 atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt acagaaaggc atttatgatc
    37261 agttcgtcaa tcggctgggt gaagcgatgc aggcggttca atttggtaac cccgctgaac
    37321 gcaacgacat tgcgatgggg ccgttgatta acgccgcggc gctggaaagg gttgagcaaa
    37381 aagtggcgcg cgcagtagaa gaaggggcga gagtggcgtt gggtggcaaa gcggtagagg
    37441 ggaaaggata ttattatccg ccgacattgc tgctggatgt tcgccaggaa atgtcgatta
    37501 tgcatgagga aacctttggc ccggttctgc cggtggtcgc atttgacacg ctggaagagg
    37561 ctatctcaat ggctaatgac agtgattacg gcctgacctc atcaatctat acccaaaatc
    37621 tgaacgtcgc gatgaaagcc attaaagggc tgaagtttgg tgaaacttac atcaaccgtg
    37681 aaaacttcga agctatgcaa ggcttccacg ccggatggcg taaatccggt attggcggcg
    37741 cagatggtaa acatggcctg catgaatatc tgcagaccca ggtggtttat ttacagtcgt
    37801 aatgagtaaa agaggcggag gttttttcct ccgcctgtgc gcgtcagagt ttagcgaatt
    37861 tttcgagggt gcgaataagc tgcgtgacga agccatattc gttatcgtac caggcgaccg
    37921 ttttcaccag ttgtaaatcg cccacggcgg taatttccgt ttgcgtggca tcaaacaccg
    37981 aaccgaaatg gctgccaatg atatcggaag agactatttc ttcatcggta taaccaaatg
    38041 actcgttatt ggtggttgct tgtttaagtg cgttattcac ctcttcggca gtcacttttt
    38101 ttccgagaat cgataccagt tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
    38161 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
    38221 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgtg
    38281 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
    38341 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aagacagtta gtggtgcatg
    38401 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattgacg ttataaacga
    38461 tagttttcat ttcaccggca ggggcggaaa tcaacacctt cttcgcacca gcatcaagat
    38521 gcgcctgcga tttctcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
    38581 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
    38641 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
    38701 aatcatgttt cagcagatag gcgagaattt ttggggaagt gagatcatta atagcgacaa
    38761 cgtctatgtt gcttttgact tcaagtaatc gacgcaacac cagtcgaccg atacgaccaa
    38821 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
    38881 gatcctttcc aggcttagcc caggattgtg ggatgggcaa tctccattct tacagtgaaa
    38941 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
    39001 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
    39061 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
    39121 cgtggtttct tcccacgtag cgatcggcca ctcatcaaca tggttcatgt ttcctgtggt
    39181 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
    39241 ccgattatac ctaagccaaa accgatgatg acgggactgg cgcatttggg acatttggtg
    39301 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
    39361 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
    39421 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
    39481 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
    39541 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
    39601 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
    39661 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
    39721 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgaac
    39781 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
    39841 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
    39901 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcctc
    39961 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
    40021 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
    40081 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
    40141 tgtgaatgtc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgtgctt
    40201 catagccttt ctccttgccg gatggcgggt aagaggctaa gatctgaatt gctaggttca
    40261 ttcgttggcc tcggttgata gaaatatcgg tcggggcctt cgtctttctg attcccggtt
    40321 agcctgaaaa cagaaagtct caggcacccg caggcatcct atgaggtttc cttagggacg
    40381 aaaataatca cttcacgaaa ttgcgtgctg ttttccagaa tttttcgtca ttcgggttag
    40441 ccagtttagc cattcgttac tctctttatt ccaatagcat taattttctc tgcaataatt
    40501 gttgtaaaaa tgtgacgcaa agaggttttt ggtcataagt aattaccgtc aagtgccgat
    40561 gactttctat caggagtaaa cctggacgag agacaacggt aatgaataca actccctcac
    40621 atcgattagg ttttttgcat cacatcaggc tggttccgtt atttgcctgc attcttggcg
    40681 gtatcttagt tctattcgca ttaagttctg ctctggctgg ctatttcctc tggcaggccg
    40741 atcgcgatca gcgtgatgtt actgcggaga ttgagattcg gaccgggtta gcgaacagtt
    40801 cagatttttt gcgttcagcc cggatcaata tgattcaggc cggggctgcg agtcgtattg
    40861 cggaaatgga agcaatgaag cgcaatattg cgcaagccga atcggagatt aaacagtcgc
    40921 agcaaggtta tcgtgcttat cagaatcgac cggtgaaaac acctgctgat gaagccctcg
    40981 acactgaatt aaatcaacgc tttcaggctt atatcacggg tatgcaacct atgttgaaat
    41041 atgccaaaaa tggcatgttt gaagcgatta ttaatcatga aagtgagcag atccgaccgc
    41101 tggataatgc ttataccgat attttgaaca aagccgttaa gatacgtagc accagggcca
    41161 accaactggc ggaactggcc catcagcgca cccgcctggg tgggatgttc atgattggcg
    41221 cgtttgtgct tgccctggtc atgacgctga taacatttat ggtgctacgt cggatcgtca
    41281 ttcgtccact gcaacatgcc gcacaacgga ttgaaaaaat cgctagtggc gatctgacga
    41341 tgaaggatga accggcgggt cgtaatgaaa tcggtcgctt aaatcgtcat ttacagcaaa
    41401 tgcagcattc actggggatg acagtaggga ctgttcgaca gggtgcggaa gagatttatc
    41461 gtggcaccag cgaaatttca gctggcaatg cggacctgtc atctcgcacc gaagaacaag
    41521 cggcggcaat cgaacaaact gccgccagca tggagcaact cactgcgacg gtgaaacaga
    41581 atgcggataa cgcgcatcat gccagcaaac tggcgcaaga ggcttctatt aaagccagcg
    41641 atggcgggca gacggtttcc ggtgtagtaa aaacgatggg cgctatctcc acgagttcga
    41701 agaaaatttc tgagatcacc gccgtcatca acagtattgc tttccagacg aatattctgg
    41761 cactgaatgc tgccgttgaa gccgcgcgag cgggtgagca agggcgtgga tttgccgttg
    41821 tcgccagcga agtacggaca ctcgcaagcc gcagcgctca ggcggcgaaa gagattgaag
    41881 gcttgatcag tgaatcagtc aggttaattg acctggggtc ggatgaggtg gcaacggcag
    41941 ggaaaaccat gagcactatt gttgatgccg tcgcgagtgt cacacatatc atgcaggaaa
    42001 tcgccgccgc ctcggatgaa caaagtagag gcataactca ggttagccag gcgatttctg
    42061 aaatggataa ggtgacgcaa cagaatgctt ctctggtaga agaggcctca gcagcggcgg
    42121 tgtcccttga agaacaggct gcacgattaa ctgaggcggt ggatgtattc cgtctgcaca
    42181 aacattctgt gtcggcagaa cctcgcggag cgggtgaacc agttagtttc gctacggtgt
    42241 gaaaatgttc aaggagggat cgacagatcc cttcaccttt cagaacggca ttgattttcg
    42301 aatagcgtta atcatcaact ggcaaccaga agagaacgtc gcatctacgc gggtcagtat
    42361 tccaatcggt tcgcctgcac catgtcccgg aacaggcagg gccaccagcg tggcatgacg
    42421 caggtcgtct tttacagcgc cagaagggac aaaccacacg taatcgtatt caaccgtaag
    42481 ttgacgagat agcgaagcag acagcgtttc gatacaaccc gaaggaattt tacagccctg
    42541 gctctgcact aatgcatctg aatgctggcg tggcgcagtg ccttctggtg atacaacgac
    42601 cggccattcc agcacccggc ttagcgttac gttctcctga agtagcgggt gattagggcg
    42661 gacaaccagc ttcaacgatt caagaaacag cagttcgtaa ttaagcccgg tcatcagttc
    42721 aggatctgac atccgaccaa tgccgatatc gatttccccg gtttttaaac ccgccagaat
    42781 catagggtta ctcattgtcg caacttgcaa ggtcgtctct ttttgttgct gatgaaactg
    42841 acctataacc gaaggtaata tccccagtgc cgcagtaggt agtgcaccaa ccctgacgac
    42901 atcattatta agaccttctt tacgatgaag cgactgtccg gcagtgttga tggcgtcaag
    42961 aactctgact gcatgcgtta aaaattgttc gccgggtaag gtaagttgcg ccccctgacg
    43021 accacgctca aacaagcgag cgccagtcag ctgctccagt tcattcaatg tcttagagag
    43081 cgcaggttga ctcaaattaa gggtttcagc cgcgcgcccc aaagttcctt gttgtgcgac
    43141 agctacgaat gtatgaaggt ggcgcaaacg gatgcgctga ctaaacagac tatttttttc
    43201 cataagcgat gttaaaaacg aagcggtgtc gctgacaagt gaagttgttt gattatgata
    43261 acttgattgc aaaatattat taacaattaa agcaattatg ttacagcaaa atggataata
    43321 ttgatgtttt cgcggcgaga tcacagtttg taaattcttc ccgcaagagt gaatgcggtt
    43381 acctacactc cagattactg accactggag gcagacacta tggcgaacag catcacggcg
    43441 gatgagattc gggaacagtt ttcgcaggca atgtcagcca tgtaccagca agaagttccg
    43501 caatatggca cgctgctgga actggtagct gatgtgaatc tggctgtgct ggaaaacaat
    43561 cctcaactgc acgaaaaaat ggtaaatgca gacgagctgg cgcgactgaa tgttgaacgt
    43621 catggggcga ttcgcgttgg gactgcacaa gagcttgcta ctcttcggcg gatgtttgcc
    43681 attatgggga tgtacccggt gagctattac gatctctcgc aggcaggggt gccggtacat
    43741 tcgacagcat ttcggcccat tgatgatgct tctctggcgc gtaatccctt ccgcgttttt
    43801 acctccttac tccgccttga gcttatcgag aacgaaattt tgcgccagaa agcggcggag
    43861 attctgcgtc agcgcgatat cttcacccca cgttgtcgac aactgttaga ggaatatgag
    43921 cagcagggcg gttttaacga aacacaggca caggagtttg tgcaggaagc cctggaaacg
    43981 tttcgctggc accagtcagc aacggtagat gaagaaacct atcgcgcctt gcacaacgaa
    44041 catcggttga ttgctgatgt ggtctgtttt cctggatgcc atatcaacca cctgacacca
    44101 cgtacgctgg atattgaccg ggtgcagtcg atgatgcctg aatgcggaat tgaacccaaa
    44161 attctcatcg aggggccgcc gcgccgcgag gtaccgattt tactacgcca gaccagcttt
    44221 aaagcactgg aagagacggt gttgtttgcg gggcagaaac agggcacgca taccgcgcgc
    44281 tttggtgaaa ttgagcagcg tggcgtggca ttaacgccga aagggcgaca actgtatgat
    44341 gatcttctgc gtaacgctgg aaccgggcag gataatctca ctcaccaaat gcatttacag
    44401 gaaaccttcc gcacttttcc tgacagtgag tttttaatgc gtcagcaagg gctggcatgg
    44461 ttccggtacc gtctgacgcc ttcaggtgag gcgcatcgtc aggcgattca tcccggagac
    44521 gatccacagc ccttaattga acgtggttgg gtcgcggcgc aacccattac ctatgaagat
    44581 ttcttgcccg ttagcgcggc ggggatcttc cagtcaaatc tgggtaatga aacgcagaca
    44641 cgcagtcacg gtaatgccag tcgcgaagca tttgagcagg cgttgggttg tccggttttg
    44701 gatgagttcc agctttacca ggaagcggaa gaacgcagta aacgtcgctg tggtttgctt
    44761 taaattctga ccatccgcct ttgcaaaaat ttgcctgatt ttacaaacga atcaggctca
    44821 tcccatcgac ataaaaaaaa tgccgattta tgcatattct ctcagttcaa caattggatt
    44881 attaataaat attgtctaga gtgagcggtc ataaataagc actttcttgc cgctgaaaac
    44941 gaccagcgcg ggaccattca caacaccaga aggactcact ttcaggtatg gatcgtagac
    45001 gatttattaa aggttcaatg gctatggccg ccgtgtgcgg taccagcggc attgcttctc
    45061 ttttttctca ggcggcattc gcggcagatt ctgatattgc cgacgggcaa acccagcgtt
    45121 ttgacttctc cattctacag tcaatggcgc acgacttagc gcaaacagcg tggcgtggtg
    45181 cgcctcgtcc gttacctgac acgctggcga caatgacgcc gcaggcttat aacagtattc
    45241 aatacgacgc cgaaaaatcg ctctggcata acgttgagaa ccgtcaactg gacgctcagt
    45301 tcttccatat gggaatggga ttccgtcgcc gcgttcgtat gttttctgta gatccagcaa
    45361 cacatctggc gcgtgaaatt cactttcgcc cggagttgtt caaatacaac gatgcgggtg
    45421 ttgatacaaa acaattagaa gggcaaagcg atctcggctt tgccggtttt cgcgtgttta
    45481 aagcccccga actggcgcgc cgtgatgtag tatcatttct cggcgcgagt tatttccgcg
    45541 ccgttgatga tacatatcaa tacggtttgt cggcccgcgg cctggcgatc gacacttaca
    45601 ctgacagtaa agaagagttc cccgacttta ccgccttctg gtttgatacg gtaaaaccgg
    45661 gggcaactac ctttaccgtt tatgcgttgc tcgatagcgc cagcattact ggtgcctata
    45721 agttcactat ccattgtgag aaaagtcagg tgattatgga tgtggaaaat cacctgtatg
    45781 cgcgcaaaga cattaaacag ctgggcattg cgccgatgac cagtatgttc agctgcggta
    45841 ctaatgaacg tcggatgtgc gatacaattc atccgcaaat tcatgactct gatcgtctgt
    45901 ccatgtggcg gggcaacggc gagtggattt gccgtccgct gaataatccg caaaaattgc
    45961 agttcaatgc ttacaccgac aacaacccga aagggtttgg tttattgcaa ctggatcgtg
    46021 acttctccca ttatcaggac attatgggct ggtataacaa acgcccaagt ctgtgggtgg
    46081 agccgcgtaa caagtggggt aagggcacca tcggcctgat ggaaatccca acaacgggcg
    46141 aaacgctgga taacattgtc tgcttctggc agccagaaaa agctgtaaaa gcaggtgatg
    46201 agtttgcatt ccagtatcgt ctgtactgga gtgcgcaacc gcctgttcat tgcccattag
    46261 cgcgcgttat ggcgacgcgt accggcatgg gcggtttccc ggaaggttgg gcaccaggtg
    46321 aacactatcc cgaaaaatgg gcgcgtcgtt ttgccgtcga tttcgttggt ggtgatctga
    46381 aagctgccgc accaaaaggc attgagccgg tgattacgct ttccagtggg gaagcgaagc
    46441 aaatcgaaat tctctatatt gaacccattg atggttatcg tattcagttt gactggtatc
    46501 cgacttcgga ctccactgat ccggtcgata tgcggatgta tctgcgttgt cagggcgacg
    46561 ctatcagtga aacatggctg tatcagtatt tcccgccagc gccggataaa cgtcagtatg
    46621 ttgacgaccg cgtgatgagt taatcgtttt ttcttcggca ccttcttcgg gaggtgccgt
    46681 ctggttaaac acgatcccgc tcgcattttt ccctaagtta aatgagtaat ctgatggtgt
    46741 gtatttcaga tacaccttgt cagccactaa cagggagtgc gtatgtttcc agaataccga
    46801 gatttaatat cccgtctgaa aaacgaaaat cctcgcttta tgtccttgtt cgataaacac
    46861 aataaacttg atcatgaaat tgccagaaag gaaggttccg acggtcgagg gtacaatgcg
    46921 gaagtggtcc gcatgaaaaa acaaaagcta cagttaaaag atgagatgct caaaatcctg
    46981 cagcaggaga gcgtcaaaga ggtgtaaact ttcctaagcc gccagccagg cggctttttt
    47041 aacaacagca tggattgact ggagataaga tgactgaaac gataaaagta agcgaatcac
    47101 ttgaattaca tgctgttgcg gaaagtcacg tcacacctct ttatcagtta atctgtaaaa
    47161 ataaaacctg gttacagcag tcgctaaact ggccgcagtt cgttcagagt gaagaggaca
    47221 cgcgaaaaac ggtgcagggt aatgtgatgt tgcatcaacg cggctatgcc aaaatgttca
    47281 tgattttcaa agaagatgaa cttatcggcg ttatctcgtt taatcgtatt gaaccactga
    47341 ataaaactgc tgaaatcggc tactggctgg acgaatctca tcaggggcag gggatcattt
    47401 ctcaggcgct gcaggcattg attcatcatt acgctcagtc tggtgaactt agacgcttcg
    47461 tgatcaaatg tcgggtggac aatccgcaaa gcaaccaggt cgctttgcgc aatggtttta
    47521 tccttgaagg ttgcctgaaa caggctgagt tcctgaatga tgcctatgat gatgtgaact
    47581 tatacgcgcg tattatcgat tcataataac cctgccagcg gcgttcgcgt aatgcgctcc
    47641 tcgccgttaa tgacttttgg accacggaca tgaaccgtgt cgccatcaaa ggcttcaacg
    47701 acggcgtggt cggttaactc cacatggttt tcaattatca ccgcacctga aatacggctc
    47761 tcaccttgta ttacaacgtg ctcgtcgagc agaatcggtc caccacgtac cacggcattt
    47821 ccgccaatca agacatgatg ttttaacaca caattacctt ccacaatggc atattccgcc
    47881 acctgcgaac tgtaatgaat cgtcggaatg gcatcttctt ctatgccagc tttcacctgc
    47941 gcgtgaccgt agactttagc gcaatcgcat agccagacgt tgttctcttc attaccttca
    48001 acactggcaa aatcaaaaac ttcggcgcga tgttcaataa aagcataccg gacgacggca
    48061 tcgccataaa tttgtgcctg gtggacaata cgcgaggcgc taacccttgc gcgatcataa
    48121 atttggagca ggagttgatg gtcgggcgtt aagccttgtg cggcgacgat catagaatgt
    48181 tgatcaatta aggcgtgacc aaatattcta cattgcccgt aaactaatga gtcatgaatg
    48241 gtgacactgt cacttatgta tgcaccctgg ctgatttcac tattatcgat ccagacatta
    48301 tccgttgcgt aaacctctcc ccacagtacg ctggtgccgg tgatgcgcgt attaccggaa
    48361 atcaccgttc cgccgaacgc aatagcattc tggtcgtaaa tccagcaatt tccttcctgt
    48421 gctaacaccg tttcgcgatc gatccagccg ccagcggttc ctgctatcac atcgttaaaa
    48481 tcgctaatgg cgataatctg ccgtaataac acattttttt tagtgccatc ttcctgataa
    48541 ctaaaggctc gctgttcttc acttagacga tatttgcgca tagcgttttc ccacaggtga
    48601 cttactataa ccgtagcaaa ttctgcggct ctggctatgc tcgagaaaat tccataaaat
    48661 gcatttcaaa tatactttat aaattaaaca aaatgagtaa gaagatgcag agcgataaag
    48721 tgctcaattt gccggcaggc tactttggta ttgtgttggg gacgataggg atgggatttg
    48781 cctggcgcta tgccagccag gtttggcagg tcagccactg gttaggggat gggctggtga
    48841 ttctggcgat gatcatctgg ggattattaa ctagcgcatt tattacccga ctcatacgct
    48901 ttccgcatag cgtgctggcg gaagttcgcc atccagtgct gagcagtttt gtgagtttgt
    48961 ttccggcaac gacgatgctg gtggcgattg gttttgttcc gtggtttcgc ccactggcgg
    49021 tgtgcctgtt cagttttggt gtcgtggttc agttggctta tgccgcctgg caaactgcgg
    49081 gattatggcg cggatctcac cctgaagaag ctaccacgcc tggactgtat ctgccgacag
    49141 ttgccaacaa ctttatcagc gcaatggcct gtggtgcgtt gggctacacc gacgccggtc
    49201 tggtgttttt aggcgcaggc gttttctcat ggctaagcct tgaaccggtg atcttgcagc
    49261 gtctgcgcag ttcgggagaa ttacccacgg cactgcggac atcactcggc attcagctcg
    49321 ctcctgcgct ggtggcttgt agtgcctggc tgagcgtcaa cggcggcgag ggtgacacgc
    49381 tggcgaaaat gcttttcggt tatggactgc tgcaactgct gtttatgcta cgtctgatgc
    49441 catggtatct ctcccagcca tttaatgctt cattctggag tttctcgttc ggcgtatctg
    49501 cactggcaac caccggtttg catctgggga gtggcagcga taatggattt ttccatacgc
    49561 tggcggtgcc gctgtttatc tttaccaatt ttattattgc aatactgctc atccgtactt
    49621 ttgcgcttct gatgcaggga aaattgttag tcagaaccga gcgcgccgtt ttaatgaaag
    49681 cagaggacaa agaatgatca ttcgtgacga aaactatttt actgataaat atgaattaac
    49741 ccgcacacac tctgaagtac tggaagcggt gaaagtggtt aaaccgggta aaacgctgga
    49801 tctgggctgt ggcaatggtc gtaacagtct ttacctggca gccaatggtt atgatgttga
    49861 cgcatgggat aaaaatgcca tgagtatcgc caacgtcgag cgcattaaat ccattgaaaa
    49921 tctggataat ttacacaccc gagtcgttga tctgaataac ctcacatttg atagacagta
    49981 cgattttatt ctttcgactg tggtactgat gttccttgag gctaaaacca tcccagggct
    50041 gattgtcaat atgcaacgtt gcactaaacc tggtggttac aacctgattg tggcggcgat
    50101 ggataccgct gattatccat gtaccgtcgg cttcccgttt gccttcaaag agggagaatt
    50161 acgtcgatat tacgaaggct gggagagggt gaaatacaat gaagacgtcg gcgaactgca
    50221 ccgcaccgac gccaacggta atcgtattaa actgcgtttc gccacgatgc tggcacgtaa
    50281 aaaatgaccc gctaagaata aggcgcgtga aaattcccca cgctgagatg atttactgtt
    50341 cttcttttcg gtaagcatat tttttatcga agggatgtga aattaatcac agtagtcgaa
    50401 gttttttagc agcttaactt actgaaattt aactactgat gattgactta gccccttttt
    50461 cggcattgac tatgtcgtct gaaaaggggc tgaaaaattt attttcacca acactttttt
    50521 tgccacaaca cgaagcggcg ctttttgcta taacttagaa agtaatataa tcatctcagg
    50581 aaactattca tgcgtaccac atcatttgcg aaagttgcag ctttatgcgg cttgttggct
    50641 ctgtctggtt gtgcctctaa aatcacccag ccagataaat attctggttt tttaaacaat
    50701 tactctgatt taaaagaaac aacctcagca acaggtaaac ctgttttacg ttgggtagac
    50761 ccgagttttg atcaaagcaa atatgacagc atcgtctgga acccaatcac ttattatccg
    50821 gtaccaaaac cttctaccca ggtagggcag aaagttctgg ataaaatttt gaactatacc
    50881 aacaccgaaa tgaaagaagc gatagcgcag cgtaaaccac tggttaccac cgctgggccg
    50941 cgtagtctga ttttccgtgg ggccattacc ggtgtagata ccagcaaaga agggctgcaa
    51001 ttctatgaag tggttcctgt tgcattagtg gttgcgggga cacaaatggc tacaggccac
    51061 cgtaccatgg atactcgcct ctattttgaa ggtgagctga ttgatgcagc gactaataaa
    51121 ccggttatca aagtcgttcg tcagggcgaa ggtaaagacc tgaataacga aagtacgcca
    51181 atggctttcg aaaatattaa acaagttatt gatgacatgg cgaccgatgc caccatgttt
    51241 gacgttaaca aaaagtagtt ccagacgcgc catcgttaga tggcgctttt tatccggtgc
    51301 gccgtaaaac cccatccttc agggcgggga tataaggcgc ggttttccac ctgaccaggt
    51361 gtttgctgtt gttcaatgta ttggcggatg acggatattg gcgcacctcc gcagctactg
    51421 gcaaaatagc caggactcca caaaacgcct ttgtagtaat acctgactgc aatatctggt
    51481 cgatctcgtc gcagtaacct actcgatacg cctttgaggc tgtttaccag actggatatc
    51541 gccagtttgg gaggatagtt aattaacaaa tggacgtgat ctggttcgcc atccatttaa
    51601 accagttcag cttcaaaatc agcacataca tttgaaaagt aagtgcgtag tttttctgtc
    51661 gcgtcatagt caaaaatctg acgtcggtat ctggtgacaa agaccaggtg aacatgcatc
    51721 aggaaaacac aatgcttccc cgtcggatat cggtttcttt attcatagac caaagtatca
    51781 tttggcctgt gaaacgatta caggcattta aattccagtt aagacccggt ggtcaacagg
    51841 agcgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt tttcaatcgt gctctggcgc
    51901 ttcagaatga gaatcatgag gccggtaata aatacatccc ttacgggaaa atggcttcct
    51961 ggctggttga gtggaaaaac gccactgaaa cgcaatggct taaagattcg ccctcacagc
    52021 cattgcaaca gtcactgaaa ggccttgagc gggcttacaa aaacttcttc cagaagcggg
    52081 ctgcttttcc ccgattcaaa aagcggggac agaatgatgc attccgctac ccgcagggcg
    52141 ttaagctcga tcaggaaaac agccgtattt ttctgccgaa actgggctgg atgcgctacc
    52201 ggaacagccg tcaggtcacg ggagttgtga aaaatgtcac tgtcagccag tcctgcggta
    52261 agtggtacat cagtattcag acagaaagtg aagtatcaac tccggttcac ccttcagcat
    52321 caatggtcgg gctggatgct ggcgtggcta aactcgccac gctgtcagat ggcacagtct
    52381 ttgagcctgt aaacagtttt cagaaaaacc agaagacgcc ggcgagactt cagcgacagt
    52441 taagccgcaa ggtcaaattc agcaacaact ggcagaagca gaaacgcaaa atacagcgac
    52501 tgcattcctg tatcgcaaat atccgcaggg actaccttca caaagtcaca acgaccgtca
    52561 gcaaaaacca cgcaatgata gtcattgagg atttgaaggt cagcaacatg tcaaagtcag
    52621 cagcgggtac ggtcagccag ccggggcgca atgtccgggc aaaatcaggt ttaaaccgtt
    52681 cgatactgga tcagggctgg tatgaaatgc gccgccagct tgaatacaag cagctctgga
    52741 gtggcggtca ggtgcttgct gttccgccag cgtatacaag ccagcgttgc gcgtgctgtg
    52801 gtcatacagc gaaagaaaat cgtctgtcac aaagtaaatt cagatgccag gtatgtggat
    52861 atacagcgaa cgccgatgta aacggcgctc gtaacatttt agcggcgggg cacgccgttc
    52921 ttgcctgtgg agggatggtg cagtcaggcc gcccgttgaa gcaggaaccc accgaaatga
    52981 ttcaggcgac agcctgaacg tagcagggat ccacgtcctt cagggcgtgg aggatgtcaa
    53041 tatcggtttc tgtcagcgat taaattcaac accacgtaac aaacgcctcc ggcaattaat
    53101 ccccagaacg cagaaccaat cccgaccagc gtcaatccac ttgccgttac cagaaatgcc
    53161 accaccgccg cgtcgcgctc acgctcatta tgcagcgcct gatacaaact gccgccaatg
    53221 gtactcaaca gcgccagacc tgccagcatc tggatccaac ttacgggcag ggcagccatc
    53281 atcccggtaa tggcactacc aaacagacct gcgagcaaat agaaaatgcc tgcaccggca
    53341 gctgccagcc aacgttgatc tttatccgga tgcgcttccg ggctttggca aatagccgcg
    53401 gtgattgccg caataccgac ggaataaacg ccgaaagggg aaaaaaccag tgccagcaat
    53461 ccagtaaata caattaatgg cgaaacagga gttgaatatc cggctgcttt cattgctgcg
    53521 atacccggtg cgttttgcga tgccatcgtc accagaaaaa gggggagtgc aacgctcagg
    53581 ctgtgagcaa acgaaaaatc aggggtaata taagtgggga gaacgggttt aaagacaaca
    53641 tcagttgtga caacgtcacc ttgcgcgata acgatcacga tcccaataat catcgcggca
    53701 attaccgcat agcgcggcgc aacggccttg gttgccagcc ataccagcaa catacttcca
    53761 cacaacgtaa attgaccgtc cagactggca aacgcctgta aaccaaagcg taataaaatc
    53821 ccggcaagca ttgccgccgc aagcgagtgc ggaataatgc gcatcagacg agcaaagagt
    53881 cccgttatgc cgcagaggac tattaacgcg ttggtgaaaa taaaaacgcc gatggcttcg
    53941 ttaagtgtta gtccctgcaa tccggtgacc aacaaagccg cgccaggcgt tgaccatgcg
    54001 gtgagaacag gtacgcgata ccataatgtc agagtcagcg tactgacgcc cattgccagc
    54061 cccagcgccg tcatccagcc agagatttgt gcagtggtgg ctccggcgac aatcgctgct
    54121 tgccagatta ttgccgctga actggcgtag ccaattaata ccgccagaaa ccccgccagt
    54181 agcgtgggtg gaggaacaga aaacagacgc atagtcacct cgtgcgttat agcgtacagg
    54241 caccgtagca tttgtccgtt ataacgcaca agtgataaac ttccgttttg ccggaggagt
    54301 cgcatggaaa atctcgctcg ctttttatcc accacactta aacaactacg ccagcagcgc
    54361 ggctggagtc tttcgcgact ggcagaagcg acgggcgttt ctaaagcaat gttagggcag
    54421 attgagcgta atgagtccag tccgacggtc gcgacattat ggaaaattgc caccgggttg
    54481 aacgtaccat tttcaacatt tatttctccg ccgcagcccg ctacgccttc agtttatgat
    54541 ccacaacagc acgcgatggt gatcacttcg ctgtttcctt atgatccaca actctgcttt
    54601 gaacacttct cgattcagat ggcacccggt gcaataagcg aatcgacgcc ccatgagaag
    54661 ggggtaatcg aacatgtcgt cgtcattgat ggacaacttg atctgtgcgt tgatggggaa
    54721 tggcagaccc ttaattgtgg ggaaggcgtt cgatttgctg cagacgtcac gcatatctat
    54781 cgtaatggtg gggagcaaac cgtacatttt cattccctca tccattaccc gcgcagttaa
    54841 gcgggaaaac tatttcgcaa cgtcgcgctt gtggctaaaa tagccgccat ttttcagcta
    54901 ctggataaga atgtgaccgt atcttctcat cgacttgaac tgttaagccc ggcacgcgat
    54961 gccgccattg cccgcgaagc tattttgcac ggtgccgatg ctgtttatat cggcggccct
    55021 ggttttggtg cccgtcataa tgccagtaat agcctgaaag atattgccga gctggtgccg
    55081 tttgcccatc gttatggtgc aaaaattttc gtcacgctta acaccatttt gcatgatgat
    55141 gagctggaac ccgcgcaacg gctgattact gacctctacc agaccggtgt cgatgcgctg
    55201 attgttcagg atatggggat tctggaactt gatattccgc cgattgaact gcacgccagt
    55261 acgcagtgcg acattcgtac agttgaaaaa gcgaagttcc tctctgatgt tggcttcacg
    55321 cagattgtgc tggcgcgaga gctgaatctt gatcagatcc gcgcgattca ccaggctacg
    55381 gacgcgacca ttgaattctt tattcatggg gcactgtgcg tggcctattc gggtcagtgc
    55441 tacatttctc atgcgcaaac agggcgtagc gccaaccgtg gcgattgctc gcaggcgtgc
    55501 cgtttgccat acacattgaa agacgatcag gggcgggtgg tttcctatga aaaacatctg
    55561 ctgtcgatga aagataacga tcagacagcc aacctcggcg cgctgattga tgctggcgta
    55621 cgctccttca agattgaagg gcgttacaaa gatatgagct acgtgaagaa tatcaccgcc
    55681 cattatcgcc agatgctgga tgccattatt gaagaacgtg gcgatctgac gcgtgcttca
    55741 tcaggtcgta ctgaacattt ctttgttcca tcgacggaaa agactttcca ccgtggtagc
    55801 acagattatt ttgtgaatgc ccgtaaaggc gatattggcg cgttcgattc gccgaaattt
    55861 atcggcctgc cggtgggtga agtattgaaa gtggcgaaag atcatctcga tgttgccgtc
    55921 accgagccac tggcaaatgg tgatggcctg aacgtgatga ttaaacgtga agtcatcggt
    55981 tttcgtgcca atacggtcga gaaaaccaga gaaaatcagt accgcgtctg gcccaatgaa
    56041 atgccagcag atttgcacaa aattcgtcca catcacccac taaaccgtaa tcttgatcat
    56101 aactggcagc aggcactgac aaaaacctcc agcgaacgtc gggtggcggt agacattgaa
    56161 ctgggcggct ggcaggaaca actgattctg accctcacca gtgaagaggg tgtcagcatc
    56221 acgcatacgc tggacgggca gttcgacgaa gccaataacg ccgaaaaagc aatgaacaat
    56281 ctgaaggatg gtctggcaaa actggggcaa accctctatt acgcccgcga tgtgcaaatt
    56341 aatttgccgg gggcgctgtt tgtaccaaac agtctgttaa accagttccg ccgtgaagct
    56401 gctgacatgc tggatgctgc gcgtcttgcc agttaccagc gcggcagccg taaaccggtt
    56461 gctgatcctg cgccggttta tccgcaaacg catctgagtt tcctcgcgaa cgtatacaac
    56521 cagaaagcgc gtgaatttta tcatcgctat ggtgtgcagc tgattgacgc ggcgtatgaa
    56581 gcacatgaag agaagggcga agtcccggtg atgatcacca agcattgtct gcgctttgcc
    56641 tttaatctgt gcccgaaaca ggcgaaaggc aatatcaaaa gctggaaggc gacgccaatg
    56701 caactggtta acggcgatga agtattaacg ctaaagtttg attgccgccc atgcgagatg
    56761 cacgtcattg gcaaaatcaa aaatcacata ctgaaaatgc cgttaccggg aagcgtagtg
    56821 gcatccgtaa gtccggatga gctgctgaaa acattgccta agcgaaaagg gtaaaacgcc
    56881 agttttctgg ttactcacaa ctcattgaat ctgcatgata ttgcctgccg ggtaaggcgt
    56941 ctcgccgcat ccggcaacaa atgactcaac gttgtcccgc ttctggtttg cgcgattttt
    57001 gccagtaaaa atgctcgcgc aaaccttccg ccgactcttc cgccacagca cgcaattcat
    57061 cgctgtccgc ttcatgacgc agctgatgat ccacattctt tacccacaca aattcatgtc
    57121 ctttgtgccc tgccatgagt tgtcctgaga acagcgcaca cgttaataag acaaccgata
    57181 acgccttcgt aaacatcctg ccaccttttt cttacctttt gccgctatga tgccgatcgt
    57241 ttcttgaggt tattattcag ttttgcaaat tagcgcaaag aaattctgga atcttccttc
    57301 ctgattttgc attgcattct gccgttgcgg cgatttagtg ctattttcga gcaaattaca
    57361 cacagaggta aacactaatg tttactttgt tgatatactc agcggcaggg aggcgatgtg
    57421 aaacaaagcg agttcagacg ttggctcgaa tctcagggcg tcgatgtagc gaatggccgc
    57481 aaccatttga aactcaggtt tcatgggagg cgcagtgtca tgccgcgtca ctcctgcgta
    57541 aagcaatcct gaaacaactc ggtttgagtt aatcgccaat taaaaaggtt aatgacatgc
    57601 gagagacagt cgaaattatg cgttatcccg tcactcttac acccgcgccg gaaggcggtt
    57661 atatggtttc ttttgtggat atccctgaag cgttgacgca gggcgaaact gtcgctgaag
    57721 cgatggaagc ggcaaaagat gctttactga ccgcatttga tttttatttt gaagataacg
    57781 agcttatccc gttaccttcg ccattaaata gtcatgatca ctttattgaa gtacctttga
    57841 gcgtcgcctc taaagtattg ctgttaaatg cttttttaca gtcagaaatc actcagcaag
    57901 agttagccag gcgaattggc aaacctaaac aggagattac tcgcctattt aacttgcacc
    57961 atgcgacaaa aatcgacgcc gtccagcttg cggcaaaggc gcttggcaaa gagttatcgc
    58021 tggtgatggt ttaattacag ttaacgaaaa gttgtcattt ttaacaactg atatagactg
    58081 ccgaatcatc tgcacataat tacgattcga taatgaaaaa ataccagcag cttgcagaac
    58141 aattacgcga gcagattgcg tcgggtatct ggcaacccgg cgatcgtttg ccttcgttgc
    58201 gtgaccaggt ggcgctttct ggcatgagct ttatgactgt cagccatgcc tatcagttgc
    58261 tcgaaagtct gggatatatt atcgcgcgac cgcagtcggg ttattacgtt gcgccacagg
    58321 caataaaaat gccgaaagcg ccagtcattc cagtcactcg agatgaagca gtcgatatca
    58381 acacttatat ttttgatatg ttgcaggcca gtcgcgatcc gtcggtcgtt ccgtttgcct
    58441 cggcctttcc cgacccgcga cttttccccc tccaacaact aaaccgctcg ctggcgcagg
    58501 taagcaaaac cgccacagcg atgagcgtga ttgaaaactt accgccagga aacgcagaac
    58561 tgcgtcaggc tattgctcgt cgctatgcct tacagggcat caccatttct cctgatgaaa
    58621 ttgtcattac tgccggggcg ttagaggcat taaacctcag tttgcaagcg gtaactgaac
    58681 cgggcgattg ggtgatagta gagaatcctt gtttctacgg tgcgttgcag gcgctggagc
    58741 ggctacggct gaaggcgtta tcggtggcga cggatgttaa agaagggatc gatcttcagg
    58801 cgctggaact ggcgttgcag gattatccgg tgaaagcgtg ctggctgatg actaatagcc
    58861 agaacccact cggatttacc ttaacgccgc aaaaaaaagc acaactggtg gcgttactca
    58921 atcagtacaa cgtaacgctg attgaagatg acgtttacag cgaactttat tttggacggg
    58981 aaaaaccgct gcctgcgaaa gcgtgggatc gccacgatgg cgttttgcat tgctcttcgt
    59041 tttcgaaatg tctggtgcct ggttttcgta ttggttgggt cgccgccgga aaacatgcac
    59101 gtaaaattca acgcttgcag ttgatgagta cgctttccac cagctcaccg atgcaacttg
    59161 cgctggtgga ttacctttcc acgcgccgat acgacgccca tcttcgtcgc ctgcgtcgcc
    59221 agcttgcgga acgtaaacaa cgtgcctggc aggcactgct gcgttatctg cctgcggaag
    59281 tgaaaattca tcataatgac agtggttact ttctctggct ggagctcccc gagccgttag
    59341 atgccggcga attaagcctg gcggcactga cgcatcatat cagtattgcg ccgggtaaaa
    59401 tgttttctac cggtgaaaac tggtcacgtt ttttccgttt taataccgcg tggcagtggg
    59461 gagagcgtga agaacaggcg gtaaaacaat taggcaaact tattcaagaa cggctgtaat
    59521 agcgtttaat ttaattcctc ttagattggg taatatgaat ttcgaatagc agtcatattt
    59581 cctaactcct tgactatact ccagaagata accttacaga cggcataatg cgcggtagct
    59641 cacaacctga ttaaattttc tcaggggcga aggtgtgcct gcaagccgcc gtctatggtt
    59701 aaacaaggag atatttttac ggcacggcgg ctgaacaatt aattacgaca ggagtaagac
    59761 cttatgagca agacatttgc ccgcagcagc ctgtgtgcgc tcagcatgac aataatgacc
    59821 gctcacgccg ccgaaccgcc taccaattta gataaaccgg aagggcgact ggatattatc
    59881 gcctggccgg gatacatcga acgcggacaa actgataaac aatacgactg ggtaacgcag
    59941 ttcgaaaaag agacaggctg cgcggtgaat gtgaaaaccg ccgcgacttc cgatgaaatg
    60001 gtcagtctga tgaccaaagg gggttacgat ctggttacgg catccggcga tgcctcgctg
    60061 cgtttgatta tgggtaaacg cgtgcagccg attaataccg cattgattcc caactggaaa
    60121 acgctcgatc cgcgcgtggt taaaggcgac tggtttaatg ttggcggcaa agtttacggc
    60181 acaccttacc aatgggggcc gaacctgctg atgtacaaca ctaaaacctt cccgacgccg
    60241 ccggatagct ggcaagtggt ttttgttgag caaaatctgc cggacggcaa gagcaataaa
    60301 ggccgcgttc aggcttatga tggccctatc tacattgcgg acgctgcgtt gttcgttaaa
    60361 gccactcagc cgcagttggg catcagcgat ccgtatcaac tcaccgaaga acagtaccag
    60421 gcggtgctga aagtgctgcg cgctcaacac agtttgatcc atcgctactg gcatgacact
    60481 accgtgcaaa tgagcgattt caaaaacgag ggtgtggttg cttccagtgc ctggccctat
    60541 caggccaacg ccctgaaagc cgaaggccag cctgttgcta ccgttttccc gaaggagggt
    60601 gttaccggtt gggctgatac caccatgctg catagcgaag cgaaacatcc ggtttgcgcc
    60661 tacaaatgga tgaactggtc attaacgcca aaagtgcagg gcgatgtggc ggcctggttt
    60721 ggctcgttac cggtagtgcc ggaagggtgt aaagccagtc cgttattagg cgaaaaaggt
    60781 tgtgaaacca acggttttaa ctatttcgac aaaatcgcct tctggaaaac gcctatagca
    60841 gaagggggca agtttgttcc ctacagtcgc tggacgcagg attacattgc cattatgggc
    60901 ggtcgctaac ttcgctgggg tgctttatga cgtacgcagt ggagtttgac aacgtctcgc
    60961 ggttgtacgg tgacgtgcgc gcagtagatg gcgtcagtat tgcgataaaa gatggtgagt
    61021 tcttctctat gctggggccg tccggctccg gcaaaaccac ctgcctgcgc ctgattgctg
    61081 gcttcgaaca gctttccggc ggggctatct ctatctttgg taaacccgcc agcaatctgc
    61141 caccgtggga gcgggacgtg aatactgtct ttcaggacta cgcgctattt ccgcatatgt
    61201 cgattcttga caatgtcgcc tatgggctga tggtcaaagg cgtgaataaa aagcagcggc
    61261 acgcaatggc gcaagaggcg ctggagaaag tggcgttggg gtttgtacat caacgtaaac
    61321 cgtcacaact ttctggtggt cagcgccagc gggttgctat cgccagagca ttggtgaatg
    61381 aaccgcgcgt attgctgttg gatgaaccgc tcggcgcact ggatctcaaa ttgcgtgagc
    61441 agatgcagct ggaactgaaa aaactgcaac agtctctcgg tatcactttt atcttcgtta
    61501 cccacgatca gggcgaagcg ttatcgatgt ccgatcgtgt ggcggttttc aataatggac
    61561 gcattgagca ggtcgattcc ccgcgcgatc tctatatgcg cccgcgcacg ccgtttgttg
    61621 ccggattcgt cgggacatcc aacgtttttg atggactgat ggcagagaaa ctttgtggca
    61681 tgacgggaag cttcgccctg cgaccggaac atatccgcct caacacccct ggtgaactgc
    61741 aggccaatgg cacgatccag gcggtgcaat atcagggcgc ggcaactcgt tttgaactga
    61801 aattgaacgg cggtgaaaaa ctgcttgtga gtcaggccaa tatgacaggc gaagaactgc
    61861 ctgccacgct cacgcccgga caacaggtga tggtttcctg gtcgcgtgat gtgatggtgc
    61921 cgctggttga ggagaggtga atggcgatga atgtattgca atcaccttca cgtccaggtc
    61981 tgggtaaggt gtccggtttc ttctggcgta atccggggct ggggctgttt ttactgctgc
    62041 ttggcccgct aatgtggttt ggcattgtct atttcggctc gctgctgaca ctgttatggc
    62101 agggatttta tacttttgac gatttcacca tgtcggtaac gccggaactg acgctggcga
    62161 atatccgtgc gctgtttaat ccggcgaatt acgacatcat tctccgcacg ctgactatgg
    62221 ctgtggcagt cactatcgcc agcgccattc tggcttttcc aatggcgtgg tatatggcgc
    62281 gctataccag cgggaaaatg aaagcttttt tttatattgc ggtaatgttg ccgatgtggg
    62341 cgagctacat tgttaaagcc tatgcctgga cgttattgct ggcaaaagat ggcgtggctc
    62401 agtggttttt acaacatctt gggctggaac cactgctgac ggcgttcctt acattacctg
    62461 cggtgggcgg aaatacgctg tcaacttccg ggctggggcg ctttctggtg tttctctata
    62521 tctggttgcc gttcatgatc ctgcccgttc aggcggcgct tgagcgtttg ccgccgtcat
    62581 tgttgcaggc gtcggctgat ctcggcgcac gtccacgaca aacctttcgc tatgtggtgc
    62641 tgccgctggc aatcccgggt attgccgctg gctctatctt taccttctca ctcacactgg
    62701 gcgattttat cgtcccgcag ctggttggtc ctccaggata ttttatcggc aatatggttt
    62761 attcccagca gggggcgatt ggcaatatgc cgatggcggc ggcattcacc ctggtgccga
    62821 ttgttctcat cgcactgtac ctggcgttcg tgaaacgtct gggagcgttc gatgcactct
    62881 gaacgcgcac cgtttttcct caaactggcg gcctggggcg gcgtggtttt cctacatttt
    62941 cccatcctga ttatcgccgc ttatgcgttt aacactgaag atgcggcgtt tagttttcca
    63001 ccgcagggcc tgacgctgcg ctggtttagc gtggcagcac agcgtagtga tattcttgat
    63061 gccgtgacac tgtcacttaa agtggcggcg ctggcgacat taatggcgct ggtgttaggg
    63121 acgctggcaa ctgccgcgct gtggcgacga gactttttcg gcaaaaacgc catttcgctg
    63181 ttactgctgc tgcccattgc gctgccgggc attgtcactg gtctggcgct attaaccgct
    63241 tttaaaacca tcaatctgga gccgggattt ttcaccatcg tggtcggtca tgcgactttt
    63301 tgcgtagtgg tggtgtttaa caatgtcatc gcccgttttc gccgcacctc gtggagtctg
    63361 gttgaggcgt caatggatct tggggccaat ggctggcaaa ccttccgcta cgtagtgttg
    63421 ccgaatctca gttcggcgct actggcagga ggaatgctgg cgtttgcctt gtcgttcgat
    63481 gaaatcatcg ttacgacttt tacggcaggt catgaacgaa cgttaccgtt gtggttgctc
    63541 aatcagcttg ggcgaccgcg tgatgtaccg gtaactaacg tggtggcact gctggttatg
    63601 ttggtaacaa ccttgccgat cctgggggcc tggtggctaa cccgcgaagg cgacaatggt
    63661 caataaccac tgatacagga atatgctatg caacataagt tactgattaa cggagaactg
    63721 gttagcggcg aaggggaaaa acagcctgtc tataatccgg caacggggga cgttttactg
    63781 gagattgccg aggcatccgc agagcaggtc gatgctgctg tgcgcgcggc agatgcagca
    63841 tttgccgaat gggggcaaac cacgccgaaa gtgcgtgcgg aatgtctgct gaaactggct
    63901 gatgttatcg aagaaaatgg tcaggttttt gccgaactgg agtcccgtaa ttgtggcaaa
    63961 ccgctgcata gtgcgttcaa tgatgaaatc ccggcgattg tcgatgtttt tcgctttttc
    64021 gcgggtgcgg cgcgctgtct gaatggtctg gcggcaggtg aatatcttga aggtcatact
    64081 tcgatgatcc gtcgcgatcc gttgggggtc gtggcttcta tcgcaccgtg gaattatccg
    64141 ctgatgatgg cagcgtggaa actggctccg gcgctggcgg cagggaactg cgtagtgctt
    64201 aaaccatcag aaattacccc gctgaccgcg ttgaagttgg cagagctggc gaaagatatc
    64261 ttcccggcag gcgtgattaa cgtactgttt ggcagaggca aaacggtggg tgatccgctg
    64321 accggtcatc ccaaagtgcg gatggtgtcg ctgacgggct ctatcgccac cggcgagcac
    64381 atcatcagcc ataccgcgtc gtccattaag cgtactcata tggaacttgg tggcaaagcg
    64441 ccagtgattg tttttgatga tgcggatatt gaagcagtgg tcgaaggtgt acgtacattt
    64501 ggctattaca atgctggaca ggattgtact gcggcttgtc ggatctacgc gcaaaaaggc
    64561 atttacgata cgctggtgga aaaactgggt gctgcggtgg caacgttaaa atctggtgcg
    64621 ccggatgacg agtctacgga gcttggacct ttaagctcgc tggcgcatct cgaacgcgtc
    64681 agcaaggcag tagaagaggc gaaagcgaca gggcacatca aagtgatcac tggcggtgaa
    64741 aagcgcaagg gtaatggcta ttactatgcg ccgacgctgc tggctggcgc attacaggac
    64801 gatgccatcg tgcaaaaaga ggtatttggt ccagtagtga gtgttacgcc cttcgacaac
    64861 gaagaacagg tggtgaactg ggcgaatgac agccagtacg gacttgcatc ttcggtatgg
    64921 acgaaagatg tgggcagggc gcatcgcgtc agcgcacggc tgcaatatgg ttgtacctgg
    64981 gtcaataccc atttcatgct ggtaagtgaa atgccgcacg gtgggcagaa actttctggt
    65041 tacggcaagg atatgtcact ttatgggctg gaggattaca ccgtcgtccg ccacgtcatg
    65101 gttaaacatt aagttagatg ttagtggctg ttataggacg gccactatcg ttttaggttc
    65161 tgagtttaat ggcggttaag tattagaatg attacattaa taactagcgt aaaaattagg
    65221 ctaaatgttt gatggcgatt tttcatcctg t
//
LOCUS       DAZFWP020000005        63856 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFWP020000005 DAZFWP020000000
VERSION     DAZFWP020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 63856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.005"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..1331
                     /gene="dgcM"
                     /locus_tag="ACHK0Z_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             99..1331
                     /gene="dgcM"
                     /locus_tag="ACHK0Z_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU4500360.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(1352..1915)
                     /gene="smrA"
                     /locus_tag="ACHK0Z_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             complement(1352..1915)
                     /gene="smrA"
                     /locus_tag="ACHK0Z_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU4500361.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(2245..3153)
                     /gene="abgR"
                     /locus_tag="ACHK0Z_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             complement(2245..3153)
                     /gene="abgR"
                     /locus_tag="ACHK0Z_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4500362.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            3329..4639
                     /gene="abgA"
                     /locus_tag="ACHK0Z_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             3329..4639
                     /gene="abgA"
                     /locus_tag="ACHK0Z_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU4500363.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGM
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            4639..6084
                     /gene="abgB"
                     /locus_tag="ACHK0Z_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     CDS             4639..6084
                     /gene="abgB"
                     /locus_tag="ACHK0Z_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU4500364.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            6118..7644
                     /gene="abgT"
                     /locus_tag="ACHK0Z_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             6118..7644
                     /gene="abgT"
                     /locus_tag="ACHK0Z_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU4500365.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            7655..8170
                     /gene="ogt"
                     /locus_tag="ACHK0Z_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             7655..8170
                     /gene="ogt"
                     /locus_tag="ACHK0Z_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU4500366.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            8365..9117
                     /gene="fnr"
                     /locus_tag="ACHK0Z_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             8365..9117
                     /gene="fnr"
                     /locus_tag="ACHK0Z_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU4500367.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            9269..10219
                     /gene="uspE"
                     /locus_tag="ACHK0Z_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             9269..10219
                     /gene="uspE"
                     /locus_tag="ACHK0Z_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU4500368.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(10269..10526)
                     /gene="ynaJ"
                     /locus_tag="ACHK0Z_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             complement(10269..10526)
                     /gene="ynaJ"
                     /locus_tag="ACHK0Z_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU4500369.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            10770..11801
                     /gene="ynaI"
                     /locus_tag="ACHK0Z_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             10770..11801
                     /gene="ynaI"
                     /locus_tag="ACHK0Z_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU4500370.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(11852..13465)
                     /gene="mppA"
                     /locus_tag="ACHK0Z_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             complement(11852..13465)
                     /gene="mppA"
                     /locus_tag="ACHK0Z_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU4500371.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(13802..14701)
                     /gene="pgrR"
                     /locus_tag="ACHK0Z_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             complement(13802..14701)
                     /gene="pgrR"
                     /locus_tag="ACHK0Z_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU4500372.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            14839..15759
                     /locus_tag="ACHK0Z_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             14839..15759
                     /locus_tag="ACHK0Z_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4500373.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            15759..16691
                     /locus_tag="ACHK0Z_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             15759..16691
                     /locus_tag="ACHK0Z_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4500374.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTFGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGVTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            16709..17347
                     /locus_tag="ACHK0Z_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             16709..17347
                     /locus_tag="ACHK0Z_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4500375.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKSFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            17656..18384
                     /gene="mpaA"
                     /locus_tag="ACHK0Z_RS01470"
                     /old_locus_tag="pgaptmp_000294"
     CDS             17656..18384
                     /gene="mpaA"
                     /locus_tag="ACHK0Z_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU4500376.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(18359..19324)
                     /gene="ycjG"
                     /locus_tag="ACHK0Z_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             complement(18359..19324)
                     /gene="ycjG"
                     /locus_tag="ACHK0Z_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU4500377.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            19443..19949
                     /gene="tpx"
                     /locus_tag="ACHK0Z_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             19443..19949
                     /gene="tpx"
                     /locus_tag="ACHK0Z_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU4500378.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(19993..21534)
                     /gene="tyrR"
                     /locus_tag="ACHK0Z_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             complement(19993..21534)
                     /gene="tyrR"
                     /locus_tag="ACHK0Z_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU4500379.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(21682..22743)
                     /gene="ycjF"
                     /locus_tag="ACHK0Z_RS01490"
                     /old_locus_tag="pgaptmp_000298"
     CDS             complement(21682..22743)
                     /gene="ycjF"
                     /locus_tag="ACHK0Z_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU4500380.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGTVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(22740..24137)
                     /gene="ycjX"
                     /locus_tag="ACHK0Z_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             complement(22740..24137)
                     /gene="ycjX"
                     /locus_tag="ACHK0Z_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU4500381.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            24292..25290
                     /gene="ycjW"
                     /locus_tag="ACHK0Z_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             24292..25290
                     /gene="ycjW"
                     /locus_tag="ACHK0Z_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4500382.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(25401..26306)
                     /gene="ompG"
                     /locus_tag="ACHK0Z_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             complement(25401..26306)
                     /gene="ompG"
                     /locus_tag="ACHK0Z_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU4500383.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(26351..27433)
                     /locus_tag="ACHK0Z_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             complement(26351..27433)
                     /locus_tag="ACHK0Z_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4500384.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(27447..28106)
                     /gene="pgmB"
                     /locus_tag="ACHK0Z_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             complement(27447..28106)
                     /gene="pgmB"
                     /locus_tag="ACHK0Z_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU4500385.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(28103..30370)
                     /gene="ycjT"
                     /locus_tag="ACHK0Z_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             complement(28103..30370)
                     /gene="ycjT"
                     /locus_tag="ACHK0Z_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU4500386.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(30367..31422)
                     /gene="ycjS"
                     /locus_tag="ACHK0Z_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             complement(30367..31422)
                     /gene="ycjS"
                     /locus_tag="ACHK0Z_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4500387.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(31432..32220)
                     /gene="ycjR"
                     /locus_tag="ACHK0Z_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             complement(31432..32220)
                     /gene="ycjR"
                     /locus_tag="ACHK0Z_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4500388.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(32238..33290)
                     /gene="ycjQ"
                     /locus_tag="ACHK0Z_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             complement(32238..33290)
                     /gene="ycjQ"
                     /locus_tag="ACHK0Z_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU4500389.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(33321..34163)
                     /gene="ycjP"
                     /locus_tag="ACHK0Z_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             complement(33321..34163)
                     /gene="ycjP"
                     /locus_tag="ACHK0Z_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4500390.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(34150..35031)
                     /gene="ycjO"
                     /locus_tag="ACHK0Z_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             complement(34150..35031)
                     /gene="ycjO"
                     /locus_tag="ACHK0Z_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4500391.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     HLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(35052..36344)
                     /gene="ycjN"
                     /locus_tag="ACHK0Z_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             complement(35052..36344)
                     /gene="ycjN"
                     /locus_tag="ACHK0Z_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4500392.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILLAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(36358..38037)
                     /gene="ycjM"
                     /locus_tag="ACHK0Z_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             complement(36358..38037)
                     /gene="ycjM"
                     /locus_tag="ACHK0Z_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HGU4500393.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFYPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(38250..38564)
                     /gene="pspE"
                     /locus_tag="ACHK0Z_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     CDS             complement(38250..38564)
                     /gene="pspE"
                     /locus_tag="ACHK0Z_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU4500394.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(38639..38860)
                     /gene="pspD"
                     /locus_tag="ACHK0Z_RS01565"
                     /old_locus_tag="pgaptmp_000313"
     CDS             complement(38639..38860)
                     /gene="pspD"
                     /locus_tag="ACHK0Z_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU4500395.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(38869..39228)
                     /gene="pspC"
                     /locus_tag="ACHK0Z_RS01570"
                     /old_locus_tag="pgaptmp_000314"
     CDS             complement(38869..39228)
                     /gene="pspC"
                     /locus_tag="ACHK0Z_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU4500396.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(39228..39452)
                     /gene="pspB"
                     /locus_tag="ACHK0Z_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     CDS             complement(39228..39452)
                     /gene="pspB"
                     /locus_tag="ACHK0Z_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU4500397.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(39506..40174)
                     /gene="pspA"
                     /locus_tag="ACHK0Z_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             complement(39506..40174)
                     /gene="pspA"
                     /locus_tag="ACHK0Z_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU4500398.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            40341..41318
                     /gene="pspF"
                     /locus_tag="ACHK0Z_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     CDS             40341..41318
                     /gene="pspF"
                     /locus_tag="ACHK0Z_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU4500399.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(41438..42703)
                     /gene="puuE"
                     /locus_tag="ACHK0Z_RS01590"
                     /old_locus_tag="pgaptmp_000318"
     CDS             complement(41438..42703)
                     /gene="puuE"
                     /locus_tag="ACHK0Z_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU4500400.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(42741..44021)
                     /gene="puuB"
                     /locus_tag="ACHK0Z_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     CDS             complement(42741..44021)
                     /gene="puuB"
                     /locus_tag="ACHK0Z_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU4500401.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(44023..45510)
                     /gene="puuC"
                     /locus_tag="ACHK0Z_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     CDS             complement(44023..45510)
                     /gene="puuC"
                     /locus_tag="ACHK0Z_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU4500402.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(45785..46342)
                     /gene="puuR"
                     /locus_tag="ACHK0Z_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     CDS             complement(45785..46342)
                     /gene="puuR"
                     /locus_tag="ACHK0Z_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU4500403.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(46369..47133)
                     /gene="puuD"
                     /locus_tag="ACHK0Z_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             complement(46369..47133)
                     /gene="puuD"
                     /locus_tag="ACHK0Z_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU4500404.1"
                     /translation="MENIMNNPVIGVVMCRNRLKDHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            47345..48763
                     /gene="puuA"
                     /locus_tag="ACHK0Z_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             47345..48763
                     /gene="puuA"
                     /locus_tag="ACHK0Z_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU4500405.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            48912..49076
                     /locus_tag="ACHK0Z_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             48912..49076
                     /locus_tag="ACHK0Z_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4500406.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            49066..50451
                     /gene="puuP"
                     /locus_tag="ACHK0Z_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             49066..50451
                     /gene="puuP"
                     /locus_tag="ACHK0Z_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU4500407.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            50585..50830
                     /gene="ymjA"
                     /locus_tag="ACHK0Z_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             50585..50830
                     /gene="ymjA"
                     /locus_tag="ACHK0Z_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HGU4500408.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            51143..52786
                     /gene="sapA"
                     /locus_tag="ACHK0Z_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             51143..52786
                     /gene="sapA"
                     /locus_tag="ACHK0Z_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU4500409.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            52783..53748
                     /gene="sapB"
                     /locus_tag="ACHK0Z_RS01640"
                     /old_locus_tag="pgaptmp_000328"
     CDS             52783..53748
                     /gene="sapB"
                     /locus_tag="ACHK0Z_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU4500410.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            53735..54625
                     /gene="sapC"
                     /locus_tag="ACHK0Z_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             53735..54625
                     /gene="sapC"
                     /locus_tag="ACHK0Z_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU4500411.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            54625..55617
                     /gene="sapD"
                     /locus_tag="ACHK0Z_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             54625..55617
                     /gene="sapD"
                     /locus_tag="ACHK0Z_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU4500412.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            55619..56425
                     /gene="sapF"
                     /locus_tag="ACHK0Z_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             55619..56425
                     /gene="sapF"
                     /locus_tag="ACHK0Z_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU4500413.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            56493..56846
                     /gene="ycjD"
                     /locus_tag="ACHK0Z_RS01660"
                     /old_locus_tag="pgaptmp_000332"
     CDS             56493..56846
                     /gene="ycjD"
                     /locus_tag="ACHK0Z_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGU4500414.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQNWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            57214..58002
                     /gene="fabI"
                     /locus_tag="ACHK0Z_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     CDS             57214..58002
                     /gene="fabI"
                     /locus_tag="ACHK0Z_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU4500415.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            58147..59274
                     /gene="yciW"
                     /locus_tag="ACHK0Z_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             58147..59274
                     /gene="yciW"
                     /locus_tag="ACHK0Z_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU4500416.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            59342..61276
                     /gene="rnb"
                     /locus_tag="ACHK0Z_RS01675"
                     /old_locus_tag="pgaptmp_000335"
     CDS             59342..61276
                     /gene="rnb"
                     /locus_tag="ACHK0Z_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU4500417.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(61354..61458)
                     /locus_tag="ACHK0Z_RS01680"
                     /old_locus_tag="pgaptmp_000336"
     CDS             complement(61354..61458)
                     /locus_tag="ACHK0Z_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4500418.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            61512..63497
                     /gene="pdeR"
                     /locus_tag="ACHK0Z_RS01685"
                     /old_locus_tag="pgaptmp_000337"
     CDS             61512..63497
                     /gene="pdeR"
                     /locus_tag="ACHK0Z_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4500419.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(63479..63607)
                     /gene="ymiD"
                     /locus_tag="ACHK0Z_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             complement(63479..63607)
                     /gene="ymiD"
                     /locus_tag="ACHK0Z_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU4500420.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            63644..63817
                     /gene="yciZ"
                     /locus_tag="ACHK0Z_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             63644..63817
                     /gene="yciZ"
                     /locus_tag="ACHK0Z_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU4500421.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT        15383 a        16597 c        15896 g        15980 t
ORIGIN      
        1 ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc
       61 ggatagcaac aaaaattgat aaaaataacg ggatctcaat gattacgcac aacttcaata
      121 ccctggactt actcaccagt cctgtctgga ttgtttcgcc ctttgaggaa cagttaattt
      181 atgccaatag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg
      241 gaccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc
      301 aacacgatat tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca gcattaagct
      361 gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt gattattttc gaaggtatcg
      421 aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac
      481 aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccct
      541 cacgagatgg ccaaatcgtc gatgctaacc tcgccgcgct caatttctat ggttataacc
      601 atgaaacgat gtgccagaaa catacctggg aaattaatat gctcgggcgt cgcgtcatgc
      661 ctatcatgca tgaaatttcg catttacccg gcggtcataa acctttgaat tttgttcata
      721 aactggcgga tggatctact cgtcatgtgc agacctatgc cgggccgatt gaaatttatg
      781 gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc
      841 agctggaaca tgctgctcac catgacgcga tgaccggatt actgaatcgg cgacagtttt
      901 atcacattac cgagccaggc caaatgcagc atctcgccat cgctcaggat tacagcttgt
      961 tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg
     1021 atgaggtgtt atgcgccctc gcccgcaccc ttgaaagttg cgctcgcaaa ggcgatttgg
     1081 tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg
     1141 cgctttcgct ggctgaaact atccgtgtaa gcgtggcaaa agtgagtatt tcgggcttac
     1201 cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac
     1261 tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc
     1321 tggcggcata agccgcggat gcgtctcgag atcaacgact gcgcttagcg tggcgctccc
     1381 agttttcttg cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc
     1441 catgatgcgg tagcgcagtg caatatgcct gaacatcatc aaattcggtc agccagcgcg
     1501 ccacatagct gcggacaata ttggcatgcg atttatcatc ccgtcctttg ccatgaataa
     1561 tcagcacgtt acgcaaacca tcagccatgg cttgttgaat gaaactgaac atcattttgc
     1621 ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac
     1681 tgcgcagctt atccagcacc ccatgttgca acccttcccg ccgaaactcc agcggctgac
     1741 ttagtgggat gatgtcgaga aatccggtgg tgaggaaatt atcaagctgc agcgtgtcga
     1801 tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatcggta gcacgtttca
     1861 gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgttac
     1921 atcctcccgc aattaagagc gcgatatgat aaccagaccg ggtcggtcca acaacgtatt
     1981 acccaaaatt ccagtaataa gttccaaata ttgccgatat tttaagcaaa atgcttatgc
     2041 atgattattc attcacgata ttaataatgt aacttatatt ttcgtgaaat ctgtcactga
     2101 agaaaattgg caactaaagg ttaaaaccgt tataacacag tcaccggcgc agaggagaca
     2161 atgccggact taagacgcgg atgcactgct gtgtgtactg tagagtctgg cggatgtcga
     2221 cagactctat ttttttatgc agttttaact ttgcagatag ccgcattctc gccggaattg
     2281 cgtgattaat gacgcggtca gtggtgtctg gcgactatca cgccgctgaa tcaaataata
     2341 ggccgctttc ggtaaaattt cgctaaccgg caacatcacc agcccctgtc cgtgcaaggg
     2401 atcgcagccc atttcttcag gcagtatgct gagaaaatca ctttttgcca ccagactgat
     2461 acaggctgag aacgtctcgc agactacacc gacctgtggc gtttgcgcct gatcgtcaag
     2521 caattcactc aactgtttgt agtaactgcc gtgtggcgtc ggcattgtcc agctgtaatc
     2581 cagtaactgt ttgatcgaac gggcaccaat ggcggggtgt cccgggcggc aaaagatcgc
     2641 gaattgcttt tccagtaatt tctcaaaagt aaattcgtgg tcgtacggtc cctgataata
     2701 ggtattgatg gtgaaatcca attctccctg acgcaattca ttaatcatcg acaccagttg
     2761 cccttccata atgcgtactt ttacctgcgg atgctgctga tgaaaacgag atatgacagc
     2821 tggcatcaga ctgcgggaaa tactggcccc catgccgata ttaatctgcc ctgccagttg
     2881 cccttgtcgt tggcgaattt cctcttgcgc tgcgcgcagc tcttcaagaa taagactggc
     2941 gtgctgataa aaactttcac cggcatcagt taacgtcacg cctttactac ggcgaaaaaa
     3001 gagttgcgcc gctaatcctt cttccagctc ctgaatagat ttactcagtg ccggttgcga
     3061 catattcaac attcggctcg ctccgcgaat gctgccctga cgagccactt caacaaaagc
     3121 acgaatttga tgaattttta cctgaaaagc catgacgcca ccgataaccg ttatttatca
     3181 gaccaaagaa actggcatct actttaatgc agatgattgt gtcagggtaa tttatgaacg
     3241 gttaaaactg tgaaaaatca gttagtgata agtaaaaact atcgctacat gaaccgggtc
     3301 acacttttta ctgatgacgg gaaaggttat ggagtctttg aatcaatttg ttaattcgct
     3361 tgccccaaaa ttatcgcact ggcgacgtga ttttcatcac tatgcagagt ctggctgggt
     3421 ggaattccgc actgccaccc ttgttgcgga agaattgcag cagctcggct attcactggc
     3481 gctgggccgc gaagtagtta atgaaagtag ccggatggga ctacctgatg aactcactct
     3541 acaacgcgaa ttcgagcgcg ctcgtcaaca gggtgcgcta gcacaatgga ttgcggtttt
     3601 tgaaggtggt ttcactggta tcgtcgccac cctggatacc ggtcgccccg gtccggtgat
     3661 ggctttccgt gtcgatatgg acgcgctgga tctcagtgaa gagcaggatg tcagccatcg
     3721 cccctaccgc gacggttttg cgtcatgtaa cgccggaatg atgcatgcct gtggtcatga
     3781 tggacatacc gccattgggc ttgggatggc gcataccctt aaacaattcg agtccggact
     3841 acatggcgtc atcaaactga tttttcagcc tgcagaggaa ggtacgcgtg gcgcgcgggc
     3901 gatggtcgat gcaggtgtcg tagatgatgt tgattatttt actgccgtgc acattggcac
     3961 tggcgtacct gcgggcaccg tggtgtgcgg cagtgataat tttatggcaa ccaccaaatt
     4021 tgacgcgcac ttcaccggga ccgccgctca cgcaggcgca aaaccagaag acggtcacaa
     4081 tgccttgttg gcggcagcac aagcaactct tgcactgcat gcaatcgccc cgcacagcga
     4141 aggagcttcc agagtaaacg tgggcgttat gcaggcagga agcggccgta acgttgttcc
     4201 tgcctcggcg ttgctgaaag tggaaacacg cggggccagc gacgtcatta atcaatatgt
     4261 ttttgaccgt gcacaacaag cgattcaggg cgcagcaacc atgtatggtg tcggcgttga
     4321 aactcgtctg atgggtgcag ctaccgccag ttctccttcg ccgcaatggg tcgcatggtt
     4381 gcaaagtcag gcggctcagg tcgcgggggt caatcaggcc attgaacgtg ttgaagcgcc
     4441 tgcgggttcc gaagatgcca cattaatgat ggcccgcgtg cagcaacatc aagggcaagc
     4501 ctcctacgtg gtgtttggca cacagctggc ggcaggtcat cacaacgaaa aattcgattt
     4561 tgacgagcag gttctcgcta ttgccgtcga aacgctggcg cgcaccgcgc tcaattttcc
     4621 ctggacgcga ggtatctgat gcaggaaatc tatcgtttta tcgacgatgc gattgaagcc
     4681 gatcgccaac gttataccga tattgccgat caaatctggg atcatccaga aacacgtttt
     4741 gaagagttct ggtcagcgga gcatctggct tcggcgctgg aatctgcaag cttcaccgtt
     4801 acccgcaacg taggcaatat cccaaatgca tttattgctt cgtttggtca aggcaaaccg
     4861 gttatcgccc tgctgggaga atatgacgcc ctggcaggtt taagtcagca agctggttgc
     4921 gcgcaaccta catccgcgac gcccggtgaa aatggtcacg gttgcggaca caatttgctg
     4981 ggaaccgccg cctttgccgc tgcaatagcc gtcaagaaat ggctggaaca atatgggcaa
     5041 ggcggcacag tgcgctttta tggttgtcct ggcgaagaag gcggctcggg taaaacgttc
     5101 atggtccgcg agggggtatt tgatgatgtg gatgcggcac tcacctggca cccggaagcc
     5161 tttgccggca tgttcaatac ccgcacgctg gcaaacattc aggcatcatg gtgctttaaa
     5221 gggatcgcag cacatgccgc gaattcccct catttgggac gcagcgccct tgatgccgta
     5281 acgttgatga ccactggcac caacttcctc aacgaacata ttattgaaaa agcgcgcgta
     5341 cactatgcca tcacaaatag cggcgggatc tcgcccaacg tggtccaggc gcaggcagaa
     5401 gtgctttatc ttatccgtgc ccccgaaatg accgacgtgc agcatattta tgatcgggtc
     5461 gccaaaatcg ccgaaggtgc ggcattgatg accgaaacca cggttgaatg ccgcttcgac
     5521 aaagcctgtt ccagttatct cccgaatcgc accttagaaa atgccatgta ccatgcccta
     5581 tcccattttg gtaccccgga atggaactcc gaagaactgg cttttgcgaa acaaattcag
     5641 gctacgctca cccccaacga tcggcaaaac agtctgaata atatcgccgc aaccggtggc
     5701 gaaaacggca aggtttttgc actacgtcat cgtgaaacgg tactggcgaa tgaggtcgct
     5761 ccatatgccg ccaccgataa cgtgcttgcg gcatcgactg atgtcggcga cgtcagttgg
     5821 aaactgcctg ttgcccagtg tttcagcccc tgttttgccg tcggtacacc gctacatacg
     5881 tggcaactgg ttagccaggg gcgaacatct attgctcata aaggaatgct gctggcggcg
     5941 aaaactatgg cagcaaccac agtcaatctc ttcattgatt cagggctatt gcaagaatgc
     6001 caacaagagc atcagcaagt tacggacacg caaccgtatc actgccctat cccgaaaaac
     6061 gtgacaccgt cacctttaaa ataacaacaa cgcaaacaca acaaccgagg aatgcccatg
     6121 agtatgtcat ccataccgtc gtcctcccaa tccgggaagc tctatggctg ggtcgaaaga
     6181 attggtaaca aggttcccca tccttttttg ctctttatct atttgataat cgtactcatg
     6241 gtgacgacgg caattttgtc agcctttggc gtcagtgcga aaaacccgac cgatggtacg
     6301 ccggtggtgg tgaaaaacct gctcagtgtg gaaggattac actggttttt acccaatgtg
     6361 attaaaaact ttagcggttt tgctccactt ggagcgatcc tggcgctggt tttaggtgcc
     6421 ggcctagcgg agcgcgtcgg cttactgcca gcgctaatgg ttaaaatggc atcgcatgtt
     6481 aatgcccgct acgccagtta tatggtgctg tttattgctt ttttcagcca catttcttcc
     6541 gatgcggcgt tagtgatcat gccaccgatg ggtgcgctga tttttctggc ggtgggcagg
     6601 catccagttg caggtttact ggctgccatt gcaggcgtag gttgcggctt tacggctaat
     6661 ttactgattg tcacaaccga cgtgttgctg tcggggatca gcacggaagc ggcggctgcg
     6721 ttcaatccgc aaatgcacgt cagtgtaatt gataactggt attttatggc cagctccgta
     6781 gtcgtactga cgattgttgg cggcttgata accgacaaaa tcatcgagcc acggttaggt
     6841 caatggcagg gaaacagcga tgagaaactg cagacattga ccgaaagtca gcgttttggt
     6901 ttacgcatag caggtgtcgt atcgctactt tttattgctg cgatcgcgct gatggtgatc
     6961 ccggaaaacg ggatattgcg cgatccgatt aatcacaccg tgatgccatc accctttatt
     7021 aaaggtatcg tgccactaat cattcttttt ttctttgtgg tttcgctggc ttatggcatc
     7081 gctacccgca caattcgacg tcaggcggat ttaccgcatt taatgattga accgatgaaa
     7141 gagatggcgg gatttatcgt gatggttttt cccctcgccc agtttgtcgc catgtttaac
     7201 tggagcaaca tggggaaatt catcgccgtg gggctgaccg atatacttga aagttcaggg
     7261 cttagcggca tcccggcgtt tgtcggtctg gcgttgcttt cctctttctt atgcatgttt
     7321 attgccagcg gttccgcaat ctggtcgatt ctggccccca ttttcgtacc aatgtttatg
     7381 ctacttggct ttcacccggc atttgcgcaa atcctctttc gtattgccga ctcatccgta
     7441 ttgcctttag cgccggtatc tccttttgtt ccactgtttc ttggattcct gcaacgctac
     7501 aaaccagacg cgaaactggg tacttactat tcgttagtct tgccctatcc gcttatcttt
     7561 ttggtggtat ggctgctgat gttgctggcg tggtatcttg ttggtctgcc gataggtccg
     7621 gggatttacc cacgtttgtc ttaagagaga acggatgctg agattacttg aagaaaaaat
     7681 tgccacgcca ctgggtccac tgtgggtgat ttgcgatgag caatttcgcc tgcgggcggt
     7741 tgaatgggaa gagtacagcg aacgcatggt gcagctgctg gacatccatt atcgcaaaga
     7801 aggctatgag cgcatttctg ccaccaaccc aggcggttta agcgacaagc ttcgtgatta
     7861 ttttgccggt aatcttagca ttattgatac gcttcccact gccacagggg ggacgccatt
     7921 tcagcgcgaa gtctggaaaa ctctacgcac tatcccctgc gggcaggtaa tgcattacgg
     7981 ccaactggct gaacaattgg gccgccctgg cgcggcgcgt gccgttggtg cggcaaacgg
     8041 atcgaatccc atcagcatcg tcgtaccttg ccatcgggtt attggccgaa acggcaccat
     8101 gaccggatat gcaggcggag ttcagcgaaa agagtggtta ttgcgccacg aaggttatct
     8161 tttgctgtaa acattaaaca atttgtgcca gcttgttcac acttttatgt aaagttaccc
     8221 ttaacaactt aagggttttc aaatagatag acatatattt acatctaata tcggaattct
     8281 ctgctgttaa ggtttgctta gacttacttg ctccctaaaa agatgttaaa attgacaaat
     8341 atcaattacg gcttgagcag acctatgatc ccggaaaagc gaattatacg gcgcattcag
     8401 tctggcggtt gtgctatcca ttgccaggat tgcagcatca gccagctttg catcccgttc
     8461 acactcaacg aacatgagct tgatcagctt gataatatca ttgagcggaa gaagcctatt
     8521 cagaaaggcc agacgctgtt taaggctggt gatgaactta aatcgcttta tgccatccgc
     8581 tccggtacga ttaaaagtta taccatcact gagcaaggcg acgagcaaat cactggtttc
     8641 catttagcag gcgacctggt gggatttgac gccatcggca gcggccatca cccgagcttc
     8701 gcgcaggcgc tggaaacctc gatggtatgt gaaatcccgt tcgaaacgct ggacgatttg
     8761 tctggtaaaa tgccgaatct gcgtcagcag atgatgcgtc tgatgagcgg tgaaatcaaa
     8821 ggcgatcagg acatgatcct gctgttgtcg aagaaaaatg ccgaggaacg tctggctgca
     8881 ttcatctaca acctgtcccg tcgttttgcc caacgcggct tctcccctcg tgaattccgc
     8941 ctgacgatga ctcgtggcga tatcggtaac tatctgggcc tgacggtaga aaccatcagc
     9001 cgtctgctgg gtcgcttcca gaaaagcggt atgctggcag tcaaaggtaa atacatcacc
     9061 atcgaaaata acgatgcgct ggcccagctt gctggtcata cgcgtaacgt tgcctgattt
     9121 ttccgcataa ctcactatcc ttctgtcata tcattaaatt tttctgattt attgatctgg
     9181 cagaaggttc atcactgttt cattcaccag atatgggtta atcttttaat tacaaactgc
     9241 gttgacagtt gttgtaagga gaccctgtat ggctatgtat cagaacatgc tcgttgttat
     9301 cgatcctaac caggacgacc aaccagcatt gcggcgagct gtttatttac atcaacggat
     9361 tggtggcaaa attaaagcct ttttgccgat ctatgacttc tcatacgaaa tgaccaccct
     9421 gctctccccg gacgaacgta ccgctatgcg tcagggcgtc atcagccagc gtacagcctg
     9481 gatccacgag caggcaaaat attatctcaa tgctggcgtt cccattgaaa ttaaagtggt
     9541 ctggcataac cgtcccttcg aagccatcat tcaggaagtg atcagcggcg ggcacgattt
     9601 ggtgctaaaa atggcgcacc aacatgaccg tctggaagcg gtgattttta cgccaacgga
     9661 ctggcatctg ttacgcaaat gcccaagccc ggtgtggatg gtgaaagacc agccgtggcc
     9721 ggaaggaggc aaggcgctgg tggcggtgaa tctcgccagt gaagagccgt atcataatgc
     9781 gctcaatgaa aaactggtca aagagacgat cgaactggca gaacaagtca accataccga
     9841 agttcatctg gttggcgctt atccggtaac gccaattaat atcgcgattg aactgccgga
     9901 atttgatccg agcgtttata acgatgccat tcgtgggcaa catttgctgg caatgaaagc
     9961 tctgcggcag aaattcggca ttaatgaaaa catgacgcac gtagaaaaag gtctgccaga
    10021 agaggtgatt cctgatttgg cggagcactt acaggcgggg attgtggttc tgggcacggt
    10081 aggacgcacc ggtatttcag cagcattcct cggcaacacg gcggaacagg tgattgatca
    10141 tcttcgctgc gatctgttgg tcattaaacc tgaccagtat cagacacccg ttgaactgga
    10201 tgacgaagaa gacgattaac gactcttcaa taactgacaa ggcccgagtt atcgggccta
    10261 ttttttactt actgctcttc gccgccaagg aaataaattc ccaacgggat tgccagcaac
    10321 acagtaaaga ccaggctata aacaaaaatc atcgatgact gaatcacata cattgatgtc
    10381 gtccactcag acagcggaat attgtactgt tctataacgc cgccgatagt cgcgcgagtc
    10441 acaaccgacg ccgcaataat gaaaaccgcc aacaaaccaa agagaaattt cttccctttc
    10501 gctgacttca gtttcgccat aatcatgatg taatccttgt gcagacaaat ttcttgttac
    10561 ctaaacgtaa caataccatg acatttcacc gttgtctgct ctgaaaacct cacctcttag
    10621 ctggatttac aatcaacgca ttgattagtt agctaatgtt gctacttcct tccctgacta
    10681 ttgatgatct gaatgaattg tcgcggtaaa atagccccgc caggggtttc cggcatgacg
    10741 acaattatct ggaggagcct gataaaacaa tgatcgctga actgtttaca aataatgcgc
    10801 ttaatctggt cattattttc ggtagctgcg cagcattgat tctgatgagc ttttggtttc
    10861 gccgcggaaa tcgtaaaaga aaaggttttt tattccatgc ggtgcaattt ttaatctaca
    10921 ccataattat cagtgctgtt ggtagcatta ttaattatgt tatagaaaac tataaactca
    10981 aatttatcac cccaggcgtt atcgatttta tctgtacgtc cctgattgcg gttattctga
    11041 cgattaagct atttttgctg attaatcagt ttgaaaaaca gcagattaaa aaaggtcgcg
    11101 atatcaccag tgcgcggatt atgtcgcgca ttatcaaaat caccattatt gtggtgcttg
    11161 ttctgcttta tggcgaacat ttcggcatga gcctttctgg cttgctgacc tttggtggta
    11221 ttggtggtct ggctgtcggt atggccggta aagatattct gagtaatttc ttttccggga
    11281 tcatgctcta tttcgaccgt cctttcagta ttggcgactg gatccgttca ccggacagaa
    11341 atatcgaagg tacagtagcg gaaattggct ggcgaattac caaaattacg acctttgata
    11401 atcgtccatt gtacgtaccg aactcgctgt tttcgtcgat cagcgtagaa aacccaggac
    11461 gaatgaccaa ccgccgcatt accacgacca ttggtttacg ttatgaggat gcggcaaaag
    11521 tgggcgttat tgtcgaagcc gtacgtgaga tgctgaaaaa tcacccggcc atcgaccagc
    11581 gacaaacctt actggtttat ttcaaccagt ttgccgactc gtcattgaat attatggttt
    11641 attgctttac caaaaccacg gtatgggctg aatggcttgc tgcacagcaa gacgtttatt
    11701 tgaagattat cgatattgtg cagtcacacg gcgcggactt tgccttcccc agccagacgc
    11761 tgtatatgga taacattaca ccgcaggaac aggggcgcta aatcagacat cactggcgtt
    11821 atccacaagg gtaacgccag ttcccatcac atcaatgctt cacaatatac atagtccggc
    11881 tgtacgccac atcttccggg ttattaatgg gataaccttt cagccacggc ttgattaatc
    11941 gtccattggt atattgataa attggtgcaa tcggtgcttg ctccatgagg attttttctg
    12001 ccgcgttgta atcggcatta cgcgctttaa cggtattttc cgtcgatgcc tgggccagaa
    12061 ctttgtcgta tgccggattg ttaaagcgtg aaatatttcc tgaatgcgtt gacgttaata
    12121 atgtcaggaa agtggagggt tcattataat cccccaccca tgaggcgcgg ataacatcaa
    12181 aattgccggt gttacggcta tcgatatagg ttttccattc ctgattttgc aatttaacat
    12241 ctacgccaag gttctttttc cacatcgatg ctacagcaat cgcaattttt tgatggtttt
    12301 ctgaagtgtt atacaaaagc gtcagcttca gcggtttttg cggaccataa ccagctgcgc
    12361 tcaacaaagt ttttgcctgc gcattcagtt cttcctgact catttgttca aacggcgaag
    12421 gttccggcgt aaatccggcg gtaacatctg gtgtaaaatg ccacgctggc ttttcgcccg
    12481 tccctaatac tttttcggtc atcaggcggc gatctatcgt catacttaat gccagacgaa
    12541 cgcgctgatc tgccgtcggc cctttttgcg tgttaaacgc ataataatag gtcccgagct
    12601 gcggcggcgt ataaacctgc cccggaatat ccttcaacag cttctgatac atatttttcg
    12661 ggaaggattc ggtgatatca atatcccccg cgaggtaacg cttagtggct gcggattcct
    12721 gattaattgg caggaaggtc actttttgca gtaccgtttt ggcgttatcc caataatggg
    12781 tattcggtac cacgaccagt ttttcattga ctacgcgttc tttaagaaca taagcgccat
    12841 tgccgatcag atttccgggt ttcgtccact ctttaccgct ttctacgttg gctttttgca
    12901 ccgggaagaa ggcaaagtta gcggttaaat tcacaaacca cggcaacggt ttatcaagct
    12961 gaattttcaa ggtatgggca tcaactgcgg tgacgccaag ctggtcaggc gtagctttac
    13021 catcaataat tgcctgtgcg ttgttgattc ccgccagcgc ggcaaaccat gcaaatggcg
    13081 atagcgtttt cgggtccacc agacgttgcc agctgtagac aaaatcttgc gccgttaccg
    13141 gtgtgccatc cgcccatttt gcgttatcgc gcagggtaaa agtccagata cggttgtcat
    13201 tacttttcca ctgagtcgca acgccgggga caatctcccc tttctcgttc tgattcacca
    13261 gtccttcaaa cagatcgcga atgacctgaa tctctggcag gcccacggct ttagcgggat
    13321 ccagcgacgc aggctcatct ttaatgtggc gcaccagctc ctgcttctct gccagtactg
    13381 tgccgctcgg aacttctgca gcatacgaaa gagaaatgct gctgaccaac agcgcacaac
    13441 acgtgactga aacagagtgc ttcataagtt tccctctaac cgacattaaa attgatgcgt
    13501 aattatttgt ttcgccttaa agaaatgcaa acaacttcca tcaaaaaagt gacacaggaa
    13561 gcataaaatg ttttatgtat caatatatta aatcagattt agtgtaaata atcgacgcca
    13621 aaatgcggca aagcacatag aacaccgaac actacgttca ggcttcgcac gttcaaacca
    13681 gttttcggta tcaaaatcag catctgtcgc cattgtacga agaataaaaa ataaccaaaa
    13741 cgttgaaagg aactgagaat acgttaagag agaaattaaa ttaatgtatc agcaagtagt
    13801 gctaaacagc acctttatac ttcagcctat ctataaataa tgagaatgcc gagccagcat
    13861 gtcgacggtg tggatagtac aggtgataac cgggtaaatc tggtgtgaat ttatccaaaa
    13921 cacgtatcag ttttttttct ttaatagccc gctcaacctg atcataaggt agatacgcca
    13981 atccatgccc atcaattgca gcatcaatga tcaggtctat cgtattcagt aaaagctgac
    14041 cttccatgcg aacacgaact tcacgtccac cgcgtattaa tctccagcga tttgctgtac
    14101 ccgatgtggg aagatacaaa ttaattgcct gatgatctat taattgtgac actgacgttg
    14161 gaacacttcg gcgagaaaaa taatccggtg agccaacaat agccattggt atatctggcc
    14221 cgattcgaat ggcgatcata tctttatcca tctccccacc cagacggacg cctgcatcaa
    14281 aacgttcaga aacgacatcg gtcaaaccat aatcaatggt gagctgaata tcaatttcag
    14341 gatgcgattt caggaatgtg cgcattgctg gtaacaatat cgtttttgct gcatgttcta
    14401 cagtagtaat acgtattgtc ccggatgggc ggttctgcag atcgctcagg gatgccatgg
    14461 ctgaatctat gtcatgcaac atagggccaa gaacagacaa aagatgctcg cccgcttcag
    14521 ttggaacaac gctgcgcgtg gttcgcgtca gaagccgcaa tcccaatcgt tcttctatac
    14581 gacgcactat ctggcttaaa gctgactgcg ccatgctcag gcgggctgct gcacgagtga
    14641 agctacgctc ctctgcaacg acgacaaacg ccatcagatc agcgatttct tcacgcttca
    14701 ttcagtatct ccatgcaatc tattaatcat ttaaatgata ggtctaatca tgattttggg
    14761 tctaatcaac ttaatgatca atatctatag tgatatcacc aaccgttaaa acgaaccgac
    14821 aactccatga ggaacggaat gatgaataat aaagtcagct tcactaacag caataatcca
    14881 accatctctt tgtctgcagt gatctatttc cctccaaaat ttgatgaaac ccgtcagtat
    14941 caggcaattg tgttatctca cccaggtggg ggcgttaaag aacaaacggc cggaacctat
    15001 gccaaaaaac tggcggaaaa gggatttgtc actattgctt atgacgcatc ttatcaaggt
    15061 gaaagtggcg gcgagccgcg tcagttagaa aacccataca ttcgtactga agacatcagt
    15121 gccgtgattg attacctgac aacgctttct tatgtcgaca atacccgaat tggtgcgatg
    15181 ggaatttgtg ccggtgcagg atatacagcc aatgctgcaa ttcaggatcg ccgcatcaag
    15241 gccatcggta ccgttagtgc ggtcaatatt ggttcgatgt tccgtaatgg ttgggaaaac
    15301 aatgtgaaat ctattgatgc cctgccatac gttgaagcgg gttcaaacgc ccgaaccagc
    15361 gatattagca gcggagaata tgccgttatg ccactggcac caatgaagga gtccgatgcg
    15421 ccgaacgaag aactgcgtca ggcctgggag tattaccaca cccctcgcgc gcagtatcca
    15481 acagcaccgg gttacgctac tttgcgcagc cttaaccaga ttattaccta tgatgcttac
    15541 catatggcgg aagtgtacct gactcagccg atgcagattg tggcgggcag ccaggcaggg
    15601 agcaaatgga tgagtgacga cctgtacgat cgggcatcaa gccaggataa acgctatcac
    15661 attgttgaag gcgcaaacca tatggatctt tatgacggca aagcctatgt ggctgaagca
    15721 atttcagtat tagctccgtt ctttgaggaa actctgtaat gaaacatatt caaatccgca
    15781 atagtgatat ggcctggcat attgctgcca atattcagtt tccaccaaat tttgatgagt
    15841 caaaacagta tccggcgatt atcagcgtcc atccttttgg cagttgtaag gagcagacgt
    15901 ttggcaatat ctatggcaaa gcattggctg aaaaaggcta tctcgttctc gcttatgacg
    15961 ccagtttcca gggagaatct ggtggggagc ctcgctggat agaagatccg acacagcgcg
    16021 tagaggatat tagccgtgtt atcgattatg cagtaactct gccatatgtt aatgctgaac
    16081 gtattggcgt gctgggagtg tgtgggggtg gtggctatgc cattaatgcc gcgctgacag
    16141 aaaaacgaat taaagcagta gtgagtatta cgggtgttaa tatcgggcgt ctttttcggg
    16201 aaggtttcag caattacgat ccgattggcg cactgaatgc aatggcgtcg caacgaacca
    16261 aagaggctcg tggtggagaa ttacagatca acgaattact tcccgccagc ctggatgcag
    16321 caaaagccca tggagtgacc gaacgcgatg tttatgaagc aactgactac tataaaacac
    16381 cacgcgggca acaaccggga ggtgcgacaa aaatgttgtt ctcacatgca cagaaaacgc
    16441 tggcctggga tgccttcgca tttactgaag tgttactgac tcagccggtg atggtagtag
    16501 ttggtgaaaa agtcggtgcc ttcggtgctt atcgtgatgg actggaagtc tatggccggg
    16561 cgatggtttc gcaggatcgt cagttagttt cattacccga tttctcacat tatgaactgt
    16621 atgacaaacc ggaagcagtt caggaggcgc tggaaaaagt tattcctttt ttcaacaccc
    16681 atctgggata agtttcatga gaaatattat gaaaaatgta ctgattcttg gtgctggcgg
    16741 tcagatagcc cgccatgtga ttaatcaact ggcagataaa caaaccatta aacaaacttt
    16801 atttgcccgt cagccagcaa aaatccacaa accctatcca actaatagca agattattat
    16861 gggagatgta ctgaatcatg cagcactgaa gcaggccatg cagggacagg acgttgtgta
    16921 tgccaacctt acgggggaag atttagatat tcaggcaaat tcagtgatcg ctgcgatgaa
    16981 agcctgtgat gttaaacgtc tgatctttgt tctttccctc ggtatttatg atgaagttcc
    17041 aggaaaattt ggcgaatgga ataatgcagt tattggtgag cccctaaaat cattccgcag
    17101 ggctgcggat gcaattgagg cttctggact ggaatataca attcttcgcc ctgcctggct
    17161 gactgatgaa gatattattg attatgagct tacctcacgc aatgaaccat tcaaaggtac
    17221 gatcgtatca cgtaagagtg tcgcagcatt aatcacagat atcatcgata aacctgagaa
    17281 acacattggt gataatatag gtatcaatca acccgtgact gatggtgaca agccattatt
    17341 catgtaaatc aattacatag ttaccatcgt aatgtactat gtgcagtacg atttattaaa
    17401 cttattaagg tgaactgatc aatccgatgc gattaacatg tgtagaacca gtaagaataa
    17461 aatacgccat gtatataaca ttacgactta catctaagtc agcataattc taatatctgc
    17521 acatcaataa aaatgcaaac aacttccggc aaaaaagtga tacaagatcg atgacatagc
    17581 gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt aatacttcct
    17641 gaaccagaga cgatcatgac cgtaaaccgc ccacgcgccg aacgcggcgc atttccgccc
    17701 ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt tcctgcccct
    17761 gcagccagtc gcgaaagtgg tttgattctg gctggcactc acggtgatga aaactcttcg
    17821 gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg ccatcatgtg
    17881 gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc taatggtgtg
    17941 gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt ttatcgctgg
    18001 aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc tggctcagaa
    18061 cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg ggtggtctca
    18121 ttccacgatc cactggcctg tattgaagat cccagacaca gcgaattagg cgaatggctg
    18181 gcccaggcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc tggctctttc
    18241 ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc gcccatctcc
    18301 tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg ctggcatcct
    18361 aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc gccagccagg
    18421 tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg gcgctaatgg
    18481 cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca cgcgcttcag
    18541 tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg ttaaccatct
    18601 catagcgccc tttcagcgcc ttcagattgc tacgtgtatg acaactctca tcagcacaaa
    18661 tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga aggggttgtt
    18721 caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac ccttctgcac
    18781 gccaggattc atttgcatca acgatcagcg tcgcatcggg cacagctgtg cgaattgcca
    18841 ccatccgctc actaataaga tggttatcca gcttcacttt cagtaatttc gcgcctgcct
    18901 gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgact accgtctgtg
    18961 cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat tgctgctgtt
    19021 ttcgcgcggc cagatcccac aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga
    19081 gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcgacacg acgctcataa
    19141 tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg cattcgccgg
    19201 tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca ctgcgacttc
    19261 cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc ttaacggttc
    19321 tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt
    19381 gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca ggaaagataa
    19441 tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat tccatcccgc
    19501 aggcgggtag caaagcgcag acttttactc tcgtagcaaa agatctgtct gacgtcaccc
    19561 tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt gataccggag
    19621 tttgcgccgc atcagtacgt aagtttaacc aactggcaac cgagatcgac aacaccgttg
    19681 tgctgtgtat ttctgccgat ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc
    19741 tgaacaacgt tatcaccctc tccactttcc gtaacgctga attcctgcaa gcctacggtg
    19801 tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg
    19861 aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag ccggattacg
    19921 aagcagctct ggctgtactg aaagcataat ttaatatgcc tgatggtgtt gcaccatcag
    19981 gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc gcaacttatt
    20041 ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt
    20101 gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc
    20161 ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca acaaaatatc
    20221 ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag cgttctttaa
    20281 ctgccgcaca tttcccggcc acgcataacg cgtaagtaca gtattcaggt cagcggccag
    20341 tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct cagttaacgg
    20401 catgatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca cgttcagacg
    20461 ataatagaga tcttcacgga acatgccttt ttgcaccagt tcgaccagat tcttctgcgt
    20521 agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc gacggaaagt
    20581 gccatcatta aggaaacgca gtaatttcgc ctgcatccgt ggtgacattt cccctatttc
    20641 atccaacagc accgaaccac cgttcgcctg ctcaaagaat cctttcttcc cttccggagc
    20701 atgaccaaac agttcactct cgaccgcatc ttccggtata gacgcacagt tcagcgccag
    20761 gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaga gatctttacc
    20821 tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg
    20881 ttcgacaaca tgcttcattt tcgggctgac ggcgacaatt tgactgaagg cgctgacgtc
    20941 ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca acatcaccac
    21001 cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg taatctccat
    21061 caggaaattc tgcccattaa taacgacatg ctcgttatgg gaatcttgcg gttcgctttc
    21121 cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt tgcgcaggcg
    21181 atccaatttt tgcgcaaaaa gctgacagct cgccgggttc gccatatcca ctttgctttt
    21241 catatcgaca gagagcacag gttcgggcaa cgcctccagt aacgcgctca acgccagatg
    21301 ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg caatacggcg
    21361 tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat tgaggtagat
    21421 tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca cgagtagatc
    21481 gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac gcatgggaac
    21541 cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat agcaggaagg
    21601 tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg taaagatatc
    21661 cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt gcagcgtttc
    21721 tttcacctga ccgataagct gacgacggaa atccccgagg cgaggtttgt catcgtcaat
    21781 ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg tcagaagtcc
    21841 agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt gcgacatcca
    21901 gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat tcagcaatac
    21961 cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca gcgtggcgat
    22021 gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca acgccagcgg
    22081 gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg
    22141 ggcatctaaa actggctgga ccaaatgcgc atacaaactg accacttcac ggtcgttttg
    22201 cgtttcatgg attgaggcat accagcgttg cagcgctgga tgagactgat caatacccgc
    22261 ctgctgcgcc agtttttcgc aaaatgcgcg gcctttgccc gtgccgtggc tgtgcaacaa
    22321 atcgcgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc gccgccactc
    22381 tgttaccaca gtacctacgc cagcgccgat aatcaatgcc ccagcggcac atccacccag
    22441 cgccacccag tcctgagttt gccaggcatt cattgtccac tgtacaccct gcccgacaac
    22501 gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc tgcgtttcgg
    22561 acgtaacgct gcatccatta ccgcttcaac ttgcccctct tcttcaggcg cttcatcgag
    22621 cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt
    22681 tggattctga tcgacctcca gcggaccgtc gaaatcaata cgtggtttta acggttcggt
    22741 catcgcaatt tatctcctat taaaaattca agcgcggcat caagacgaat atgcggcagc
    22801 ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc ttgcttgtcc
    22861 cagaacgcct gaccgggcaa acgcgcagga acttcaccgg gataaacggt gagtggcgca
    22921 ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac gtcaataatg
    22981 ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat cccttcaaac
    23041 gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat attggcgtgc
    23101 tgatcgatgg tcacatggtc cgctttagtg gcagcaaaca atagcttatc gataaccggc
    23161 gaaaacaaac gccggaacaa ggtacgctgc ccgtagtgga aactttgcat cagctgcgtc
    23221 agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg caggcaatca
    23281 accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc cttcaccacc
    23341 ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg cttatcggcc
    23401 tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa ttgcagcgcg
    23461 ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa gtgcagcccc
    23521 tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc cagccggttt
    23581 tcgtcggcag gcgctagcgg gtccagccct tcgcacatca ttcgccattt gaccgaccat
    23641 tcgccgcgct gaccattgag taagcccgtc atctggcgtg accagcttaa atagtcctgc
    23701 gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatctc cagatacagc
    23761 gtggaggtgt ccttgaagtg gcgtagcagc gaatcgttcg atttaaaacg tagcgccagg
    23821 cggatttcac tgacgccgcg cgttggcgtc ggccaggcgg gaggatcgcc atataactgc
    23881 gccagtcctt catcatatgt gaagcgcgga atgccaaagt cacgctgagg aatgcgtttt
    23941 acgcccagca ggcgctcttc acgcaccgcg cttaacagcg gcaagcgtgc tccggcgtga
    24001 atattgagca actgattgac catcgcagtg ataaacgctg ttttgccgct gcggcttagt
    24061 ccggttacgg cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc attaagttca
    24121 tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa aatcattcgt
    24181 aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg tcatcgacgg
    24241 gcgaactcca taagatgtgc agcattcacg ttcggacgga gttcctgacc tatgtcccct
    24301 actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc acgcgtgctg
    24361 aataagcaaa ctaatatctc cccggaagcg cgcgaaaaag tgttacgggc cattgaagaa
    24421 ttacagtatc agccaaacaa gctggcccgc gcgctgacct cttcgggttt tgatgccatt
    24481 atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt ctctgaagtt
    24541 ttgcatgcca tcaccgccaa agctgaagag gaaggtttcg acgtgatatt gcagacgtcg
    24601 cacaatccgg cagaagactt acaaaaatgt gaaagcaaaa ttaagcagaa aatgattaaa
    24661 ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact tgataaatac
    24721 gatattcctg tcgtggtgat tggcaaagtt gaaggtcaat atgcccatgt ttattctgtc
    24781 gataccgata attttggcga cagcattgcg ttgaccgatg cattaattga aagtgggcat
    24841 caaaatattg cctgcctgca tgctccgctt gatgtccatg tttcagtgga tcgggtaaat
    24901 ggttataagc agagcctggc tgcgcataat attgcagtgc gtgatgaatg gattgttgac
    24961 ggcggttata cccatgaaac agcactgcaa gccgcgcggg aattattaag ccagtcaccg
    25021 ttgcctgagg cagtgtttgc tactgacagc ctgaaattaa tgagtattta tcgtgcggca
    25081 gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggttatag taatgaaacg
    25141 ctgtcgttta ttttaacgcc tgcgccaggc ggtattgata tcccaacaca ggagttaggt
    25201 caacaaagct gtgagttatt attccgctta atttctggaa aaccgtcacc acagaatatt
    25261 accgttgcca cgcatatgac gctgaaataa ataccagcta ccttgcctga atgccggatg
    25321 cgatgctagc gcatcttatc cggcctacat ttatagttcg gataaagtac tcgtgccgca
    25381 tccggcaatt tagcccatta tcagaacgag taatttacgc cgacacctgc ataatggaat
    25441 ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg cgtattccag cgaaacggaa
    25501 agcccgttct ggaaatcata accgtaaaat aaacctacac ggttaaaatc atggccttca
    25561 cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat gcgcgtatac
    25621 ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc ttgcgtagaa
    25681 aactcaccgt tattgcggct gtcgtccata ttaaagccgc gctcgagata atagttcact
    25741 cgcaaggcaa ccgtttcgtt gaaggtatat tgcaggcctg tttccgtttc gactcgggta
    25801 tcagcgtaac cggtcgtgtt cagatcgttg gcaaatttat acatcgacaa ccaaccgttg
    25861 aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg cgattttcca gcgctgcata
    25921 ttcgcagtgt ctttacccgg ttcatcaacg tagtgataac cataattacg gaaaccgccg
    25981 gtcaggccga aactgaaatc atcgttttcg aggaactgat aatgcacctc cagctccggg
    26041 cgatcaaacc acgttccacg tttacccgcg ctataatcta ccggcccttc ctgataatag
    26101 gccagagcaa ttctccacgg cccgttggcg gcattaaaat agactgaagg ctccgccagc
    26161 ccatccatat cttcgccata accctcgacg ttttctattt cgtacatcgc gccgatatta
    26221 aagtgccagt cgttcctttc ctcggcctgt gcgcaggcca ttcccgcaca catcaccagt
    26281 gcggtacagg gtaataactt tttcattatt gttccttgta ttattctggg ggaatgcctt
    26341 ccccctgtat ttagcgaatt gctatttccg tttctgcatc aaagaaatga catttcgtca
    26401 tatcaaaatg aatagtgata ttttctcctg catgataatc atttaacgca ccagcacgga
    26461 ccactaactc gtgcccccca accgtggtgt agagcataaa ttccgcaccg gttaattctg
    26521 ccacgctaat tttggcggaa atgttatttt cctcttgcgc gtccggatga atatcttccg
    26581 gacgtattcc catcacgatg ggcttatgca aacttccctg tgttttcaga accgctaatt
    26641 tctcttcggg aatggttaat ttaagcgttt ccgtaacgaa tttatcgcca tcgatcgtgc
    26701 cacgaataaa attcatcgcc ggtgatccaa taaatccggc aacaaacata ttcgctggtt
    26761 ggttataaac cgttttcggc gcaccaactt gctgaacaat cccatccttc ataatcacaa
    26821 tccgcgtcgc catggtcatc gcttcggtct gatcgtgggt cacgtagatc atggtggtat
    26881 tcagcttctg gtgtagcttg ctgatctctg cgcgcatttg cacgcgcagc ttggcatcaa
    26941 ggttagagag cggttcatcc attaaaaaca cgcccgcttc gcgcacaatc gcccgcccga
    27001 gcgccacacg ctggcgttgc ccgccggaaa gcgcgcccgg cttgcgtttc aggtactcac
    27061 gcaggccgag aatttgcgcc gcccagttca cccgctcatc aatcacctct ttggcgattt
    27121 tttgcatctt caggccaaac gccatgttgt cgtaaaccgt catatgcgga tacaacgcgt
    27181 agttctggaa caccatcgct atattgcgtg ctttggctgg aacgtcattc attcgtttgc
    27241 cgtcgatcaa cagatcgccg ccgctgatct cctcaagccc ggcaatcatg cgcagggtgg
    27301 tcgacttacc gcagcccgac gggccgacaa acacgatgaa ctctttatcg gcaatttcca
    27361 ggttgaagtc cttcaccaca tgcacctggt tatcgtagat tttttgaata tgttgtaacg
    27421 aaagctgagc catgttgatt cctttgctat acgttttgcc agaaggccga taaccgcgac
    27481 caggtcagag agtccgttga aggcaacagt aattgcgccc cggttaagcc cgcgccgatc
    27541 cccaccgagc gcataccgct ggcgttaatg gcgtcaatgc ccgcctgcgc atcttcaatg
    27601 ccgatacatg cctgcggcgg cacgcccagc ccggcacagg cggcgagaaa gatttccggg
    27661 tccggtttcg agtttttaag ttgggaagca tccgcgcaga aggtgaaaaa ctcgcgcagc
    27721 tccagcgccg ctaaaatcgt cggcgcattc agggagacag aagccagccc aaccgggatc
    27781 tgctgtgcac ggagatctgc cagcaaagag cgaatgccgg gtagaacagc gctgaccgtc
    27841 aactcgcgca gtgagtggac atagagcaga tttttgcgat acgccagttg cgccctctcc
    27901 tgcgagttaa agtcgccctc tttgcccccg tgttgcagaa tgcgccgcag agactcatcg
    27961 cggctgatcc cttttaggga ttcgttaaac tgcgcatcaa tgctgatgcc aatttcagcg
    28021 gcaatctgct gccacgcctg gaaatgcaga tgcgcggtat cggtgattac accatccaga
    28081 tcgaaaatta ccccttgcag tttcattcat cctcctgatg tgtggtagcg gtcccattga
    28141 agggcaaaat aaaatcaccc aaacagaaaa cagattcttc agccacggat ataagctgac
    28201 cgttcaaacg cagtgaaacg ggcgcagaag ttcgaatcgc aatacgctgc gcgtcaagag
    28261 tgacctgtaa ttcgcagccc tgccagaaca gagggaaaga caactgttgc cactgctcag
    28321 gtaacgccgg attgagatgc aattcaccgt cacgcacgct caccccggca aaaccctgaa
    28381 tcgcccccag ccagatagcg ccagttgcgg cagcatggat accatcatca caactatgcg
    28441 gatcagcacc aagatcgatt tcagtcccct cgcgccagaa ctgataactt tgggtcagca
    28501 ggccacagcg tgcggcaaca atgccgtgga ttgctttact taatgacgag tcgtgaatag
    28561 tgcgcggttc ataaaattgc agattggcaa gacacgatgc cgctgagaac tgctccggca
    28621 gcatgtaatt gagcatcacc acatcagctt gtttgaggat ttgcatctcg ttcacttctg
    28681 cgcgtgaata atccagcaga atggtttgct tccccgccgc cgctttgtat ttcgccagat
    28741 taatcgccgg cttagccata aacgaatcat cctgcggcaa aacgccgtcg ggctgaattt
    28801 ctggcagccg cagttcttta aggaacattt cggcgcgatg gataaacgca tcgtcgctac
    28861 agccaaactg gcgggcaata ctcagcgcct gctggacgtt gtaatacgcc atatagctgg
    28921 tgaaggcgtt attattgaca tgttcggtat attcgtcagg cccaataaca tcatgaattt
    28981 ccagacggtc gttaaccctc accgcgcggc taatccagaa ctttgcagtt tccagaagta
    29041 gcgccatgcc ttcatgcgca atgaaacttt catcccccgt ggtctgccag tattgaataa
    29101 ccgcccaggc gatatcggcc accagatgat gttccgcctg cgccgaggcc actttttgcc
    29161 gcagcccggt gcgaatgtta atggcggcaa attccggcgt ctcttcttcg ccgctgcgcg
    29221 cgctttccca cggaaatagc gcgccctgcc agccgttgcg tcgcgctttc tcctgcgcgc
    29281 ctggcaagtt gtgccagcga taacgcagta aacttcgggc aaccgtcgga tcgctaaaca
    29341 gatgaaacgg taacaaaaat acttctgtat cccagaaaac gtggcctttg tagccttcgc
    29401 cggttaagcc ttttgccgca atgctgctgc gctcgtcgtg ggcaggcgtc atgatgcgca
    29461 gatgataaag cgcataatct aacgcttgct gatcgtgcgc ttcgccgcca tttaccgtga
    29521 tacgacgttt ctgccaccat tgacgccagt tttctgttga tactgcaaga agttggtcgt
    29581 aactctgctg cgcgcacatt tcaagctggc gaagcgacgc gctgccccac tcgtctaaaa
    29641 cggcttgcct gtcatcccgc cagtcgatcc acaccagttt ttgcaacgtc actgtctcgc
    29701 ctgcatgaag ctgcgcactg gtatgttgct gcaaacggcg ctctttggcg gtatagcatt
    29761 gctgcacatc accgctcacc atacagcaac agctgatggc cacatcactg cgtccatcct
    29821 gggtggtgta gatcccctgc atcagatgct gaccaaacac ccgcacctgg gtttcgtcga
    29881 gatgttggcg accgtggttg gtttgcgtgg cgtcgatacc tgttgaaatc agcactgacg
    29941 cgtcggcgtc cagtggcgta atagtgattt ccagcacaat gagcggcagt tggtctgccg
    30001 aaacaaagcg acggctggcg atggtgtaac ctgtgccgtt gctggtacgc cagacaacgc
    30061 tacggcgtaa ttctccactg gcaaagtcaa gttcacgctg ccaggcttcg cgggataacg
    30121 agaaaacctc accatttatg gcaatctcca tccccaagat atcaggcagg ttcaccagtt
    30181 cgttgatttc accttttccc gcccgatgat acagccccgc cagatacatc cctcgcgtct
    30241 ggcgcgtgta atcttcttca tggctggcgc gaagcccaag atagccgttc ccctgcgcca
    30301 tcagcgatgc atacttgttc agggtatgct ggctgaaatg gggttctgat aacgttactg
    30361 gcctggtcat aattccacac gcgtccctgt ttctgcggat tgatacaacg ccgccaccag
    30421 ttgctggatg atgtacccct gctcggcgtc ggctatcatc acgggcttgc cctgtacgtg
    30481 attgataaag gcttccatgc tgcgcaaatg gcggttgtcg tctgccagtt cccgttgcat
    30541 cagcgtcatt aattcaccgt tgttatcggt gtagatatgt gctggaaaca gcgtcgcacc
    30601 ggctttatca ccacaaaagc tgacgttcat aatcgactgt tcgcggatgt tgagtgcaaa
    30661 tgacgtttcc agccacagaa tgccgccgtt atgaaattca atggtgccaa ataacgaatc
    30721 ttcgacgctg taagttgccg gatcccactc gccaaattga ccgcagctct tttgcgtgcc
    30781 gatcttttga aagctatgcg cattcacgct tttcaccgcc ggaaagccca gcacatacat
    30841 cgccgcatcc agcatatgaa tgccgatgtc gataagcggg ccaccaccct gcagttcttt
    30901 attggtaaag acaccccagc cgggaacgcc gcagcgacgc agggcgcggg cggtggtaac
    30961 gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc agctgttgcg tatcgagcgc
    31021 aaaacggtgg tgaaagtcgt aggccagcac cttgcccagt ttgcgcgcgg tatcgcacat
    31081 ttcccgcgcc tgttctggcg tcatggcggg cggtttttcg cacatcacat ggcagcccgc
    31141 ttccagtgcc atcagggtat gttcgtaatg aaaacggtta ggtgagcaga cgctaaccac
    31201 atcaggtttc accgccagca gcatggcctg cggatcgtcc cacacggatg cattaccgta
    31261 tttatctgcc agcgcctgcg cctgggaaag gcggctgtca cagacagcca ccagttccag
    31321 atcgttgcgg gtgcagtagt acgaagcatg aaccttatcc gccacctggc ctgcgcctat
    31381 tatcgcgacc cgcagcggag agcttgtcat tgcacttttc acaaagacct cttagcaggt
    31441 acgcaaccag gccaacgaat cacggtacgc ctgggcagga tcttccgccc ggatacgccc
    31501 ttcgtacact acatagccct gatagttatc cgcgcgcagc tgttcaaaca gcgcgtggaa
    31561 atccagggtg ccgctgcccg gctggtagcg atggttatcc gcaatatgca catgaccgag
    31621 caggtcgcgg ttgtcatgca gcgcctgcgc caggttatct tcttcgatat tcatgtgata
    31681 gaaatcgccg ataatctgta catgtttaag atcgttttcg acgatgtaac ggcgggcatc
    31741 ggcgagggtg ttgatcatat gatcctgata gcggtttaac ggttcgagat acaccacggt
    31801 tccggtacgc gcggcgacct gttccagtac gtgcagggaa tcactcacca ttttgcggtc
    31861 gccgtccagg ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc
    31921 tgccggaacg acgatacctt taccccccac ttctgccagc gcttcgagaa tgcgttcgat
    31981 ctgctttaag ccattaagac gacgctcttc gataaagtcg ccaatccaac cgtcatagcc
    32041 accgcaggcg gtggtcaccg gtaaaccggt ttctttgatt gctgctttga cttcttcgag
    32101 attgttgacc agcagtttgc cgtcaatctc aaaaccatcg aagcccatct ctttgatata
    32161 acgaaatttc tccagaatgt tttccggaaa gaatgcctga ttctgtgtgc ctattttcat
    32221 tttttcattc ctttgaatta aaacgtaacg cccattttga tgctctgttc cggatgctga
    32281 tcgacatact gcatgtagct ttccgggctc ttggcaaagg tcactaccgg gtcgattaaa
    32341 tcttcgcaat tgagataacc gttcatcagc agttcccagc aggtttcttc aatacgctta
    32401 cggctccagc gcggataatc cgggttcggt tcgctgcacg cacgggagaa aacaattttg
    32461 gcgttattga aatgcgcttc gcgtccgagg ttaaaacctt cagcaaatgg tttggcaaac
    32521 gcgacataag agatggtgcc gccataagca aggccacgca gcgccgattg cagcgcgtcg
    32581 gcgtaaccgc tggtttcgat aatcacatcg gcaccctgct tgccggtcag cgttttgatc
    32641 tcttttccta cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca
    32701 atatcacagc gataggcgat aggatcgacg ccaatcacca ccgacgcgcc agcgcgtttc
    32761 gccagttgga tggctatttg accaatcgcg ccaagcccta ccaccaccac aaaatcaccc
    32821 acgcgcacgt tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacaaacg
    32881 gcgtttttcc acgagctgcc ttccggcatt ttgcgcagct tgtagttatt caccgcgtta
    32941 atgatgaccg tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtcg
    33001 gtaacgtcgc tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg
    33061 ccaaactcaa tgccgcgcgg cgcatctgcc ggacgcggag tgaacatctg ccattcgccg
    33121 ttaaagtctt catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt
    33181 ttcggtgcgc cgaaacgcac gcggattttc acttcattgg ctaaaatcgc ccgatcttca
    33241 tactcaacca gcgcagcaac acgcggtgct gtggctacta actttttcat ttaaaactcc
    33301 ttgtaaagaa actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata
    33361 aaacgttccg aaagcgcata cataatcacc accggcaatg cggtcaccag tgaggccgcc
    33421 atcatccgtc cccagatgta atctggcgta ctgaacagcg cgttcaggcc caccggtaag
    33481 gtgaaattgc tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgccacc
    33541 ataaagcagt agacgaacac ggatatcagc ccggacatcg ccaacggcac ggtgatgcgg
    33601 aagataattt gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcgggg
    33661 atggtgtcga agtagctttt cagcataaac acggcggtgg gcagagtttg cgtcaccatg
    33721 gtgatgatca gcgccatctc ggtgtcgtaa atgcccagcg cggtgatgat tttgaacagc
    33781 ggcaccacca gcaaaatgcc agagaacata tacaccgtgt aaaagctggc attgatggtc
    33841 atccgacctt taaagcgcag gcgggaaagc gcgtaagccc caagaatgcc gaggaatacc
    33901 gccaccacgg atgaaaccac cgacaccacc agactgttac ggaagtagtc gacaaacggg
    33961 aaaatcatcg ggttaaaaat gtcgacgtaa tgctccagcg tccactgctg cggcagcagc
    34021 gtaggatgca gtgagatcgc ctctttcgcg cccttgaacg aggtcatcag catcacaaaa
    34081 aatggaaaca gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatgcgactg
    34141 agtgtgcgtt tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc
    34201 gaaaataatg atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc
    34261 aaaggcggtt ttgtagagat atacaccgag aatatcgacc ttggtcgtca gcaaataaac
    34321 atccgcgaac atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaggaccgg
    34381 cataatcgcg ggcagcgtga cgatacgaaa ccgttgccag gcattagcgc catccatctc
    34441 tgcagcttcg tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc
    34501 atacgggaag tagcgccaga tggcgaacag caccaccagc acaaagctac tgcccggatt
    34561 atcgaaccac agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac
    34621 aatgccgtag ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt
    34681 tacgtaggag aggatcacca gcgaacgcgc ggttttgcgc agatggaact cgcggttgaa
    34741 aaacatcgcc actgccagcc cgagcacggt gctgcccgcc accaccagcg cggtatacca
    34801 gaccgtcatc cacagcgagt gccagaagcc gggatcggag aggatacgca catagttact
    34861 caccccaaca aaggttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc
    34921 gatattcgac accattggcc aggccaccag accacccagc agtaataagc tgggcgcgag
    34981 aagcagcagc gcaaagggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc
    35041 ctgtcctggg tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc
    35101 gtgccaggga gatcggcttt acccacggtg acgttatgca ccatggagct caccactcca
    35161 gaccccgtca catcacccat gcgggtaaag tttttatccc ctactgcacc aaagacctga
    35221 atatttggca gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg
    35281 ttgtctttcc aggtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc
    35341 gacatcatca cccagtcggc aatgttgtct gcctgctcca taaaggtgac aaatttttct
    35401 gctgcttccg tctcttcggt cttttgcccg gcggtaatgg tcagcgaggt caacatgccg
    35461 tagaccgcag agtttttctc ggttggcacc acgaaaccga cgtttttcgg gtcgccttct
    35521 ttaatcacag ccagaaggat ataggtggag taaatcgcca tcggcgcggt gccgttcata
    35581 aaggcgtctt tcacttccat gatgtcgtta gaacccggca tagtgttggc agcaaggtcg
    35641 cggtaatagg tcagtgcctg catcatctct ggtgtatcaa gggtgatttt gccttcggcg
    35701 ttaaagacgt tagcctggtt ggataacgca aactgggaga aggattgttc cgtcaacacg
    35761 ctttctgctg taggcagcgc aatgccgtat tttttattcg ccgggtcatt cagtttctgt
    35821 gcaacgtcca gcagctgttg ccagtttttc ggctcctcaa gccccgcttt tgccagcaca
    35881 tctttgcgat accagatacc gccaatccag gcgctgacag gaacaccggt ccatgcgcta
    35941 ccatcttcgg tacgcacaat acgcagtacg ccatcgtaaa acgcgccttc accaacgtta
    36001 ctgatgactg tggcaaccgc tttgcgatcg ataagctgct ctttgtccat cactttggcg
    36061 tagtcatggc tggtttcgat cacttccggc agcgaaccgc tacgtgaaag agtaatgact
    36121 ttagtgttat aggcatcttc ttccacgggc acttgcttaa cggtgatgcc agggttttct
    36181 ttttcaaaac gggcaatcaa tttactgata acggcctggc gctcctgctc caccgaagaa
    36241 tgcataaatt cgatggatac attcgtttta ttttcttctt tacagcctga aatcagggcg
    36301 caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg ttccttttta
    36361 tttaatccac ataacctgcc aggggcgcag cgttatttca ttacccaata tatcaacttc
    36421 actaatcaga tcccgaccat gcagattagt aatatttaca tgctgaatat ttttactgac
    36481 attaaacaat ccagtcagac aattaccatc agcgttactt ctttgaatac gcattacgga
    36541 tgaattaatc gtatcgatgg taaaattatt atccggataa aactcgttat ggctgcgacg
    36601 aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag cttcatcgtt
    36661 cagttctcgg gttatctctt tactgtgata ttttttacgg ttaatcgcac ggttatatcc
    36721 gagtttttcg acacctgcgt aatcattacg cgagccaaga atactttgaa tatatatcgc
    36781 tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg cgcaacgttc
    36841 ttcatcgcta ctctcacggc ggcttaacgc atccatatag gtcacattta tttcatacgg
    36901 actgcgtgta ccgtcgggat tatttttcca gtttactaat gcaccttcct gctgtaacgc
    36961 ctcgaccagc tctaatattt cgctttcagg caacaagccc cgtagcgggt ttagcccgat
    37021 gccatcgtga gaggcgagga agttaaacca ggtggtgttg ctggaaggta gtgtcaggtt
    37081 ttgcgcccac gcacaaagcg cctcaacgtt ctgtttttgc accgcatgca gcaccagtgg
    37141 cggcagcgag aactggtaca ccatatgtgc ttcgtcatcg cctgcgccaa agtaagcaat
    37201 gttgtcttta tgcggaacat tggtctcggt aatgatcact gtacctggcg caacgttatc
    37261 aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga tgcagctttt
    37321 tcccggctct ttccacataa agccaacggc atccaggcgg acatactcag cacctttcgc
    37381 aaggtaacag agtaaaacat ccaccatcgc cagcaacact tcagggctac ggtaattcag
    37441 gtcaatttga tcgtcactaa aggtggtcca taaatggcgc gttgaatgat cgcgcatctg
    37501 gaatggcgtt aataacggta acgcacgcgg gcgagtgacg gcgctgagat cggtttgcgg
    37561 gtcaacggca ataaaaaaat cttcaaaacc tggatgctgt tgtaaatagt ttttaaacca
    37621 ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatgac tgcattcacc
    37681 gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat aatcaattac
    37741 cgaaaagcca tcatcagatg accatggata aaacggcaac aaatgaacat gtgaaaaaat
    37801 gctttgcagc cattgatggt aaaactgatt aaatgtgggt aatggtttta aatcattgct
    37861 gtgaaattga tcggcatagg taattaacac gacatcactt tcatcccagt gttttttacg
    37921 tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct gtaaatgagt
    37981 tgcggtaaat gttccaccgt agatttcgtc caggtaatcc gtaattttct gtttcatgtt
    38041 gcctttccgt ggtagcggtc ccatgaaacg agagactact ccgcagaaaa taaactgtca
    38101 attgagcaca agggttttag tcacgcagga aatcattttt taccagggaa aaagcgtatt
    38161 tgtgagctga cgcaaataat ctatggggat gtaaataagg tgtgacagaa aaaaaaacgg
    38221 cgcataagcg ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct
    38281 ttcaggccac cggcgttctc aacgtgggta tatcccatct cgctaaggat ctcttttgct
    38341 tgccctgact ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg
    38401 gcggtggcaa tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc
    38461 tcttgctgat actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc
    38521 agtgaaaaca ccagtgccag agcaagtaag ccttttttaa acatgtaagc gtccttctat
    38581 ggatgaaaat tgccatccat tatacgcctg agatttaaca atttttgcga aactcccctc
    38641 accttttata acgctgtgcc agtttattag cagcccgact taacagcggt tccagcgcca
    38701 cagccagcaa cattttcagc ggtcggcgag caactgattt tatcgcccag cccgccacac
    38761 ccgccgggcc atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaaaccag
    38821 gctttacctt ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg
    38881 gaaacggcta cgtaacgtga aagtatcgga agtgacataa cgttccatct cgcgtaaacg
    38941 cgtttcactt gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggaaggtag
    39001 ctgctcacca aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta
    39061 agcaaccagg gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca ggatacgcac
    39121 cagttttgcc ggtacatcaa aatagttggc aatcccggcg cagacgccgc ggaccatgcc
    39181 ctgctgtgga atacgccata attttttatt gagattaatg cccgccatta gcgatccctc
    39241 cagttcggat gttctgcatc aagaatagat tccagcgcct gaatacgttc gcgcatccgt
    39301 tttgcttcat cagccagttg cgctaatcgc tgctgctcac tttgcgacaa ttcactgcga
    39361 ccagaacgat tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg
    39421 gttaacggaa tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc
    39481 gcgttcggac gccgcctgga tgttattatt gattgtcttg cttcattttg gcttttaatt
    39541 gtgccagttg ttcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg
    39601 atttttgttt accgaagctg tggctttctg cttccgcttc catctggtca atacgacgtt
    39661 cgaaagattc gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca
    39721 catcgcgcga cgagtttgcc gcctgatgac gtaacatcaa tgcctgctgg cgagcgcgtg
    39781 tttcgctcaa tttgttttcc agctcaccga tctctttctt catgcgtgcc agcgtgtcgt
    39841 ccaccagcgt cacttcatgt tccaggctct taatcagatc ggtcagtttc tgtttttcaa
    39901 ttaacgctgc acgtgccaga tcctctctct ctttcagcag cgccagttcg gctttttcct
    39961 gccattcaac ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg
    40021 ccaacgcgcg cgccgaagta gaacgtactt caaccagtgt atcttccatc tcctggatca
    40081 tcagacgaac cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat
    40141 tcacgatgtc ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt
    40201 caggattgtc ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt
    40261 tataaagatc tgattgaaga atcaacagca acatgccagg atgagttagc gaattacact
    40321 aacaagtggc gaatttcatc atggcagaat acaaagataa tttacttggt gaggcgaaca
    40381 gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca
    40441 tcatcggcga acgcggcacc ggtaaagagc tgattgccag ccgcctgcat tatctctcct
    40501 cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa aatctgctgg
    40561 attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatcctg
    40621 ggagatttga acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga
    40681 tgatggtgca ggagaaatta ttgcgcgtga ttgagtacgg tgaactggag cgcgttggcg
    40741 gcagccagcc attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg
    40801 cgatggtcaa tgaaggcact tttcgcgctg acctgctcga ccgactggct tttgatgttg
    40861 tacaactgcc accactgcgc gagcgcgaaa gcgacataat gttgatggca gaacactttg
    40921 ccatccagat gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg gagcgcgcca
    40981 gagaaacatt gctgaattat cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg
    41041 aacgttcagt gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc
    41101 cctttaaacg gcgtccgcct gaagacgcta tcgccgtttc agaaaccacc tcgcttccaa
    41161 cactgccgct ggatttacgt gagtttcaga tgcagcagga aaaagagttg ctgcaactca
    41221 gtttgcaaca agggaaatat aaccagaagc gcgcggctga attactgggg ttaacttatc
    41281 atcagttccg cgcgttgttg aaaaagcacc agatttagcg aagttgagca atacaacttg
    41341 caggatgcgg cgtgaatgct ttattcggca tacataatca atacgtcgtt tgcaggcctg
    41401 ataagcgcag cgcatcagac attattgcgt tgggctatta atcgctcagc gcatcctgca
    41461 aaattttcat tgccgcatcg aattgcacat ccgggatggt cagcggatac aggaagcgaa
    41521 tcacgttgcc gtatgcgcca caggtcagca ggagcagccc ctgcgccagc gcgcgttgct
    41581 ggattttctg tgcaatcgcc gctgacggct tgcccgtttg cggatcgtta aactctgccg
    41641 caatcatcga ccccaggccg cgtaccgcag caatggccgg aacgctttct ttggcatcaa
    41701 tcaacgtgtt tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt gattctttgt
    41761 cgataatatt gagcaccgcg tgcgcggcag ccaccgccag cgggttcccg gcgtaggtgc
    41821 cacccaaccc gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc acgcccgaaa
    41881 gcggcatccc gcccgcgagg cttttcgcca tcgtcattaa gtccggctta tcggcgtaat
    41941 gatccatggc aaacagttta ccggtacgcg caaagccgct ttgcacttca tcggcaatca
    42001 tcacaatacc gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa
    42061 cgttgaaacc gccctcgccc tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt
    42121 cgatgtctga tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt
    42181 gtaaatctga cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt
    42241 tgtacggcgc aacttttccg gtcagcgcca tggtcatata tgtacgaccg tggaagccac
    42301 cgctaaacgc aatcacgcca ggtcgtccgg tatgggcgcg agcaattttc accgcgtttt
    42361 ccaccgcttc cgcaccggta gtgaagaatg cggttttagc ctgcccgctc actggggcaa
    42421 gggcgttaat tttctccgcc agcgtgacgt agctttcgta cggcacaatc tgatacgccg
    42481 tgtgggtaaa ctgttgcaat tgctgctcca ccgccgcgac cagatcaggg tggcgatgcc
    42541 cggtattcag caccgcaatg cctgcggcga aatcgatgta ttcgttgccc tctacgtcct
    42601 tcagcgtagc gttttctgcc gactgggcga agaagttaca catcacgcca accccgcgtg
    42661 gggtagcaga aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac
    42721 tcttcactgt taatcggaaa ttaaacgccc agacgatcgc gcaggctgta ataagccgcg
    42781 cccatcgcgg taaatggcac gcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga
    42841 ttggcgaagg catcgaaacg ttcggcgtcg ccgcgcagca gttcggcaat cagacgcccg
    42901 gcaagatggg tacaggtgac gccgtggccg ctgtagccct gcatgtaata gatgttggta
    42961 tcaaggcgac cgaactgcgg catgcgcgac aaggtcagca ggaagttgcc cgtccagcgg
    43021 taatcaattt tcacgccctt cagctgcggg aaggttttca gcagtttcgg caccacaagg
    43081 cgctcaacgt catccgggtc gcgcgcgccg tagaccacgc cgccgccgta cagcaggcgg
    43141 ttgtcggcgg taagacggta gtaatcaagc agatagttac agtcttccac acagtagttt
    43201 ttcgggatca gcgaacgggc taaatcttcc gacagccgtt cggtggtgat cacctgcgtg
    43261 ccgcacggca tgctgcgttt cgccagttcc ggctctactt tatcgcccag atacgcattc
    43321 ccggcgacaa tcacatactt cgccgtcacc tgacctttgg cagttcgcac aacggctggc
    43381 gtggtgtgct ggatctgcgt cacggcagaa agttcataca cgcgcccgcc gttgaggcgg
    43441 atggcgtccg cttcgccaat ggcgaggttt agcggatgaa tatgcccacc gctgtgatcc
    43501 agcagcgcac cggtatagcg atcgctggct acttcacggc gaatggcgtt ggcgtccagc
    43561 aattccagct gtttattgcc gtagcgttcc cagttctctt tctgctcttc aagtgtggcg
    43621 agctgtttat cattcatcgc cacaaacagg ccgccggggc ggtagtcgca gtcaatctga
    43681 taacgtttga tacgttcgcg gatgatctca ccaccttcga acatcatgct gccgagcata
    43741 cgggcggtgt ccatgccgta gcttttttcg atcacgtcga tgtcgcggct gtaggagttc
    43801 acaagctgcc cgccattgcg cccgcttgcg ccaaagccga tgcgtgaggc ttcgagaacc
    43861 actacgtcaa agcccgcttc cgccagatgc agcgcggaag agagcccggt atagccgccg
    43921 ccgaccacgc aaacgtcgca ggaaatcgac tcattcagcg tgtcgaatgg cgcgtattta
    43981 ttcgcactgg cggcgtagta actgcttgta tgttcggtca tttcaggcct ccaggcttat
    44041 ccagatggtt ttcagttcgg tgaatttttc aagggcatgc agggatttgt cgcgaccgtt
    44101 gccgctctgc ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt agttattgac
    44161 gaagacggaa ccagccttca ggcggcggct catgcggtgc gcgcgggaga ggtcgcgcgt
    44221 ccataccgcc gcgccaaggc cgtactggct gtcgttggca agctgtagcg cctggtcttc
    44281 tgatgtgaaa cgcgtgacca ccagcaccgg accgaaaatc tcttcgcggc ttaaggacgc
    44341 attcgggtct acatccacaa agatggtcgg gccgatggcg gcagccagct cagcgttacg
    44401 accatccagc agcagttgcc ctttgctttc gccttcccga ataaagctat ggaccgagtc
    44461 ggcgtgggcg cagtcgatta aggtgcccat ggtggttgcg ggatcaagtg gatggcccgg
    44521 ctgccagttt tgcgcctgct gttttaacag ggctaagaat tcatcggcga tgctctcttc
    44581 cagcaacaaa cgcgttccgg cgatgcacac ctggccctgg ttgtagaaga tgcctgcggc
    44641 ggtggcgctt gccgcttttt gcagatccgg acaatcggcg aaaacgaggt tggcgctttt
    44701 gccgcccgct tccagccaga cgcgtttcat gttgctgtcg cccgcatctt tcagcagctg
    44761 tttcccggta cgggttgaac cggtaaaggc aatggcgtcg atatcgttat gacgcgacag
    44821 cgcctgcccc gcttcatgac caaaacccgt caccacgttc aacacaccat ccggcaagcc
    44881 tgcttctttc gccagccccg cgagacgaat cgcactgagc ggtgattttt cagacggttt
    44941 tagaatcacg ctgtttcccg ccgccagcgc cgggccgagt ttccagcaag tcagcaacag
    45001 cgggaagttc cacggcacga tggcggcaat cacgccgacc ggttcacgca cgatcatcgc
    45061 cagctcatgg ctactggtgg tcgccacttc gccatacact ttgtcgatcg cttcggcgta
    45121 ccagcgaatg gcgcgcgccg cgccgggaat atcatcacgc agactgtgac gaatcggttt
    45181 gccggtgtcg agagtttcca gcagtgccag ctcttcggcg tgggcttcca ttaaatcggc
    45241 gagtttattc agtaccgctt tacgtttagc cggagaagag agtgaccagt cgccgcgttc
    45301 aaatacgccg cgtgctgcgc tcaccgcacg gtcgatatcg acgctcttgc cgcgggcaat
    45361 tttcgccagc ggtgcctggg tgaccggatc aacggtttca aaggtttcat tttccgccgc
    45421 agcagtatat tcaccgttaa taaataagcg gttttcaatg gcgagactta acgctttatc
    45481 ctgccagtaa gccagatgat gaaaattcat tatgactcct gtttcacgtc tatcagatat
    45541 atgcagtttg tgaacgtcag ggcgcagaga tccccgggcc gtatgcccgt ttatttctgg
    45601 cgacattctt tatatacact tcatccttca agccgcctct gccttggctg cgttcgctcc
    45661 cccgaatcac ttacctgagt aagctcatcg ggattcgctc acttgccgcc ttgatgcaac
    45721 ttgaatgatt ttgtgtatag attgccgggc gcattgcgct tacccggctt acagaacaaa
    45781 aagattaaaa cgtggtgggc gtatgggcgc tgataattcg gcaaatacct gccgacgtat
    45841 tactgaaact gtgcgggatg ccggtattaa tggcataact ttgccccgcg acgaggtggt
    45901 aatcctgacc attaatcgtc agaacaattt caccttccag tactgtgcct atttcctcac
    45961 cctgatgctt aattctttcc ccagtggttg tgcccggctg gtacgtttca aagatcatcg
    46021 ccagcgtgcg attcgggtta ccattatgaa ccagcttcat tgacacaccc tgactgccca
    46081 tctcaattaa gtcgtcctga ttaatgacga cctgcggctc atcaggtttt tccggctcgg
    46141 aaaagaattc cgagagtgac agaccataca ccttcagcag cttttgcagc gtactgatgg
    46201 cagggctgac tttatcttgt tctatcgtac tgatagcact gtgagtcagc ccggagagtt
    46261 cggcggcacg acgttgtgaa agcccctgct gctggcggat ttccgacaag cgttttcctg
    46321 gcgccagtcc ctcatcactc atatttgcat ttccttaaac tgtagtggtc agagtcgctg
    46381 tttttcagcg atatggtgct gacaagcggt gataaagccc tcgaacaata tacgcgaaag
    46441 cgcgtactcg ctactgttcc attccgggtg ccactgtacg cccagcgcaa aaggatgatt
    46501 gatgacgcta accgcctcaa ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg
    46561 gctaacgacc ttcgcccctt gcccatgcag agagtttacc caaaagttgc tacattcagg
    46621 taacaacgca gacagtaatc ccccctcttc aacctgaact tcatgcgacg gtgcatattg
    46681 ctgttccacc ggcagttcgg gatcttcccg atgttccagc aattcaggct gttcgcacag
    46741 cttgcgatgc aacgaaccac cggttgccac caccagttcc tgtaaacccc ggcagatggc
    46801 gaaaatgggg atgcgccttt cgagtgcggc attaattaac gccatgctca gaagatcacg
    46861 cccgggatcg gcgtcaggct catcgccgtt ttcaccatat aggtgcggct gcacattgct
    46921 gggactacca ggaagataaa tgccatcgag tttcggcaaa agttgttcaa gtaatgacgg
    46981 ttccgccagc gcatgtggta gcgcaattgg caagccgcct gcgtggatga tggcattcag
    47041 gtacttttct tgcagagtct gggtcgcatg atccttaagc ctgttcctgc acattacgac
    47101 accgataacc ggattgttca ttatattttc catgctcaat ctcacaaagt ggactaaatt
    47161 atcgccatta ctgcggttta aatgaccttt atgttcaata ttttttcaat ctagcagtgg
    47221 gttttcattt ttgcaaactc aatttaacat ttgacaaaca tttggtttgc atacagattc
    47281 gaatggtggt cattatattt tacgctttga taacgagcgg aaaacaaacc aaaggcgaag
    47341 aatcatggaa accaatatcg ttgaagtaga gaactttgtt cagcagtcag aagagaggcg
    47401 gggtagcgcc tttacgcagg aagtgaaacg ctacctggag cgctacccga atacgcaata
    47461 tgttgatgtt ttacttactg atttgaatgg ttgcttccgt ggaaaacgta tccctgtttc
    47521 aagcctgaag aagttggaga aagggtgtta tttcccggcc tcggtgtttg caatggatat
    47581 tctgggcaac gtagtagaag aagcgggtct gggtcaggaa atgggcgagc cggatcgtac
    47641 ttgtgttcct gttctcggtt ccttaacccc ttctgccgcc gatccagagt ttatcggtca
    47701 gatgctcctg accatggtcg atgaagatgg cgctcccttt gacgttgagc cgcggaacgt
    47761 tcttaaccgc ctgtggcagc agctacgcca gcgcggattg ttcccggttg tagcggtaga
    47821 gctggagttc tatttactgg atcgtcagcg cgacgctgaa gggtatctgc aaccgccctg
    47881 cgcgcccggc accgatgacc gcaatacaca aagccaggtt tactccgttg ataacctcaa
    47941 ccacttcgcc gacgtgctca atgatattga tgaactggcg cagttacagt taattcccgc
    48001 cgacggtgcg gtcgccgaag cctcgccggg tcagtttgaa atcaaccttt accataccga
    48061 taacgtgctg gaagcctgcg atgatgcgct ggcgctaaaa cgcctggtgc gtctgatggc
    48121 agaaaagcat aagatgcacg ccacttttat ggcgaagccg tatgaagagc acgcgggcag
    48181 cgggatgcat atccatatca gtatgcaaaa taatcgtggc gagaatgtgc tttctgacac
    48241 agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc
    48301 gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc agccgggaat
    48361 gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc accgtcgccc tgcgtattcc
    48421 gtgcggcgac cgtcataatc accgcgtgga atatcgcgtg gcgggtgccg atgccaaccc
    48481 atatctggtg atggcagcga tttttgccgg tattttgcat ggccttgata acgagctgcc
    48541 gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa ggcttaccct tcccgattcg
    48601 ccagagcgat gccctaggtg agtttatcga gaatgatcac ctgcgccgct atttaggcga
    48661 acggttttgc catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct
    48721 catcacagaa acagaaattg agtggatgtt gaaaaacgcg taattcccca tgcggggttg
    48781 tttcatcggc ctgagcggag ccaggcctga catttgctgg taatccagac atttcccgga
    48841 tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta acgattaaga ctgtgacgag
    48901 gaaatgagat gatgccgatg attaaatccc cgcatggcga gggaggctgc gtatgcgccc
    48961 ctcctgcgac tgactggact ccgccccctt tgcttccctt gctaaacagg tttgatttca
    49021 ggtcgacacg accgcaaacc ttattacgca ggggaggcag caattatggc tattaattca
    49081 ccactgaata ttgctgcgca acccggcaaa acccgtctgc gaaaatcact gaaattgtgg
    49141 caggtggtaa tgatgggtct ggcctatctc acgccgatga ccgtatttga tacttttggt
    49201 attgtctccg gcattagcga cggtcacgtt ccggcgtcct atttgctggc gctggcgggc
    49261 gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc agtttccgga ggccggttcg
    49321 gcctatacct acgcgcaaaa gtcgattaat ccgcacgtcg gatttatggt cggctggtca
    49381 tcactgctgg attatctctt tttgccaatg atcaacgttt tgttggcgaa aatctatctc
    49441 tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta
    49501 accgccgcga atctgaagag cgtcaacctg gtcgctaact tcaataccct gtttgtactg
    49561 gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg ttcagggact gcataaagga
    49621 gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc gcacctgatc
    49681 ccgattatta ccggtgcgac gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc
    49741 acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc cgaaagccat cttcctgacg
    49801 gcggtatacg gcggcgttat ctttatcgcg gcgtcgttct ttatgcagct gttctttccc
    49861 gatatcagcc gctttaaaga cccggacgcc gcactgcctg aaattgcgct gtacgtcggc
    49921 ggcaagctct ttcagtcgat tttcctctgc accacgtttg tgaacacatt agcgtctggc
    49981 ctggcttcac atgccagcgt gtcgcgtctg ctgtatgtga tggggcgtga caatgtgttt
    50041 ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga ctccggcact caacgtcatt
    50101 atggtcggga ttgtcgcgct gtcggcgctg ttctttgatt tagtcaccgc gacagcactg
    50161 attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc
    50221 tggcggcgta aaggaatgaa taaaagctgg aaggatcact tccactattt gctgatgccg
    50281 ctggttggcg cgctgacggt gggtgtgctg tgggttaacc tagagtcaac gtcactgaca
    50341 ctcggtctgg tatgggcttc gttaggcggc gcatatttgt ggtatctgat ccgccgctat
    50401 cgcaaagtgc cgctgtacga aggtgacaga acgccggtga gcgaaacgta atagccggat
    50461 gcgcggcgca ttatcctgcc tgagggtaac gcgccgcgcc attaactata aaaagatgta
    50521 tgacccggat cacgagtgct atcatccgcc cttatgctta gcgcattaac cccaggggca
    50581 aataatgaac cacgatatcc cgctaaaata ttttgatatt gccgatgagt acgcgaccga
    50641 atgcgcagag cctgtcgcag aggctgaacg cacaccgctg gcccactact tccagttact
    50701 gctcacccgt ttgatgaaca atgaagagat cagcgaagaa gcccaacatg aaatggctgc
    50761 tgaagcgggg attaatcccg tacgcattga tgagatcgca gagtttttga atcaatgggg
    50821 taatgagtaa ttatttaatt gcagacgatc ggtcagtatc gttgttcgga taaagcgttc
    50881 acgccgcatc cggctaacaa tgacacgcac tgcagcgtgc tttaatgggt ttctcacgat
    50941 cgtaccgctc tgctcctcgc taagttgttc tgtgagttat agttatagag aaatcacggg
    51001 tacgaatgtt gaacagatgg cgacgccacc tgttcaacga ccaaccacac ggccctcatt
    51061 acgacccctg aacacaaacg gtaatggcaa atgtcattaa atattagtaa gatagcctct
    51121 ttacataaaa tcccttaata ttatgcgcca ggtattatcg tctcttttgg tgattgctgg
    51181 acttgtgagt ggtcaggcaa tcgccgcgcc tgaatccccc ccgcatgctg atatccgcga
    51241 cagcggtttt gtctattgcg tcagcgggca agtcaacacc tttaacccat ccaaagcgag
    51301 cagtgggtta attgtcgata cccttgccgc ccagttttat gatcgactgc tggatgtcga
    51361 tccctatacc tatcgcctga tgccggaact tgccgaaagc tgggaagtac tcgacaacgg
    51421 cgcgacctat cgcttccacc tgcgtcgcga tgttccgttt caaaaaaccg actggtttac
    51481 tcccactcgt aaaatgaatg ccgacgatgt ggtgtttacc ttccagcgaa tttttgaccg
    51541 caacaacccg tggcataacg tcaacggcag caacttcccc tacttcgaca gcctgcaatt
    51601 tgccgataac gtgaaaagcg tccgcaaact ggataatcat accgttgagt tccgactcgc
    51661 ccagccggat gcttcttttt tgtggcacct cgcaacccat tatgcttcgg tcatgtcggc
    51721 agaatatgcc cggaagttag agaaagaaga tcgccaggag caactcgacc gtcaaccggt
    51781 cggcaccggt ccgtatcagt tgtcggaata ccgcgccggg caatttattc gcctacaacg
    51841 tcatgatgac ttctggcgcg gtaaaccgtt aatgccgcag gtagtggtgg atttaggctc
    51901 cggcggcacc ggacgtctgt cgaaactcct gaccggggaa tgcgacgttc tggcctggcc
    51961 tgctgccagc cagctatcca ttttgcgtga cgacccgcgc ttgcgtttaa cgctgcgtcc
    52021 tgggatgaac gtcgcctatc tggcatttaa caccgccaaa ccgccgctaa ataatcccgc
    52081 tgtccgccat gcactggcac tggcgattaa taaccagcgc ctgatgcaat ccatctatta
    52141 tggtacggct gaaacggcgg cctctatttt accgcgcgcc tcgtgggcct atgacaacga
    52201 ggctaaaatt actgaataca atccggcgaa atcgcgcgaa cagttgaagg cgttggggct
    52261 ggaaaactta acgctgaaac tgtgggtgcc cacacgttcg caggcgtgga accccagtcc
    52321 actgaaaact gccgaactga ttcaggcgga tatggcgcag gttggcgtaa aagtggtgat
    52381 tgtgccggta gaaggtcgct ttcaggaggc gcggttgatg gatatgagcc atgatctgac
    52441 gttatccggt tgggcgacgg acagtaacga cccggacagt ttcttccgtc ctttactgag
    52501 ctgcgcggca attcattcac agaccaacct cgcccactgg tgcgatccga aattcgacag
    52561 cgtgttgcgt aaggcgctct cctcgcagca gctggcggcg cgtattgaag cctatgacga
    52621 agcgcagagt attctggcgc aggaattgcc cattttgccg ctggcgtcgt cattgcgttt
    52681 gcaggcctat cggtacgata tcaaaggtct ggtacttagc ccgtttggta acgcctcctt
    52741 tgctggggtg tatcgcgaga aacaggatga ggtgaaaaaa ccatgattat cttcacctta
    52801 cgccgcattt tgttattgat tgtcaccttg ttcctgctga ccttcgttgg cttcagcttg
    52861 agctatttca cccctcatgc gccgttacaa ggcgcgtcac tgtggaatgc ctgggtgttc
    52921 tggtttaacg gcctgatcca ctgggatttt ggtgtttcca gcattaacgg tcaaccgatt
    52981 gccgaacagt taaaagaggt cttcccggcg acgatggaac tgtgcatcct cgccttcggt
    53041 tttgcgctga ttgttggcat ccccgtgggg atgattgccg ggattacgcg ccataagtgg
    53101 caggataact tgatcaacgc catcgcctta ctgggctttt caatcccggt gttctggctg
    53161 gcgcttctgt tgacgctgtt ttgttcactc acgctaggtt ggttgcccgt ttcagggcgt
    53221 ttcgatctgc tctacgaagt gaaaccgatt accggttttg cgttgattga tgcctggctt
    53281 tcggactcac catggcggga tgaaatgatc atgagcgcaa tccgccatat gatattgccc
    53341 gtgatcaccc tgtcggttgc gccaacaact gaagtgatcc gcctgatgcg tatcagcact
    53401 atcgaagtgt atgaccagaa ctatgtaaaa gcggcggcga cccgcggttt gtcgcgcttt
    53461 actattttgc gtcgccacgt tctgcataac gcgttaccgc cggttattcc tcgcctgggc
    53521 ttacagtttt ccaccatgct gacgctggcg atgatcaccg aaatggtttt tagctggcca
    53581 ggtttagggc gctggttaat taacgccatc cgccagcagg attacgcagc catttccgcc
    53641 ggagtgatgg tgtgtggctc actggtcatt attgttaacg tgatttctga tattttgggt
    53701 gccatggcta accctctgaa acataaggaa tggtatgcct tacgatagcg tatacagcga
    53761 aaagcgcccg ccgggcacgc tgcgtaccgc ctggcgcaaa ttttatagtg acgcctctgc
    53821 gatggtcggc ctgtacggct gcgcggggct ggctgtactg tgtatttttg gcggctggtt
    53881 tgcgccttac ggcatcgacc agcaatttct cggttatcaa ttattgccgc cgtcatggtc
    53941 gcgctatggc gaagtttctt tcttcctggg gactgacgac ctcggacgcg atgttttaag
    54001 ccgtttactg agcggagctg cgccaaccgt gggtggcgca tttgtggtga cgcttgccgc
    54061 gacgatctgt ggcctggtac tgggcacctt cgccggggcg acacacggcc tccgctcagc
    54121 ggtgcttaac catattctgg ataccttact ggcgatccct tcgctgctgt tggccattat
    54181 cgttgtagcg tttgccggac cgagtttgtc ccacgctatg tttgctgtct ggctggcatt
    54241 gctgccgcgt atggtacgtt cgatttacag catggtgcat gacgaactgg aaaaagagta
    54301 cgttatcgcc gcccgtctgg atggcgcatc aacgctgaat attctctggt ttgctgtgat
    54361 gccaaacatc accgccgggc tggtcaccga gatcacccgc gcactgtcga tggcaattct
    54421 cgatatcgcc gcgctgggct ttctcgatct cggcgcacaa ctcccctcgc ctgaatgggg
    54481 agcgatgctc ggtgatgcgc tggaactgat ttatgtcgcc ccgtggactg tcatgctgcc
    54541 aggtgcggca attatgatta gcgtgttgtt ggttaacctc cttggcgatg gcgttcgtcg
    54601 tgcgattatt gcgggggtgg aataatgcca ttactcgata ttcgtaacct gaccattgaa
    54661 tttaaaaccg gtgatgagtg ggttaaagcc gtcgaccggg taagtatgac cttaaccgaa
    54721 ggtgaaatcc gcggtcttgt tggtgaatcc ggttccggca agagtttgat tgcgaaagcg
    54781 atttgtgggg tgaataaaga taactggcgt gttactgctg accgtatgcg ttttgatgat
    54841 atcgatttgc tgcgtctctc cgcacgcgaa cggcgcaaac tggttggcca taacgtgtcg
    54901 atgattttcc aggaaccgca gtcgtgtctt gacccttcag aacgtgtggg tcgccagttg
    54961 atgcaaaaca tcccagcctg gacctataaa ggccgttggt ggcagcgttt tggctggcgc
    55021 aaacgccgtg ccattgaact gctgcaccgc gtggggatta aagatcacaa agatgcgatg
    55081 cgcagttttc cctatgagtt gaccgaaggt gaatgtcaga aagtgatgat agccattgca
    55141 ctggcgaatc aaccgcgtct gctgattgct gacgaaccga ccaactcaat ggagccaaca
    55201 acccaggcgc aaatctttcg cctgctgacg cgtctcaacc aaaacagcaa taccactatt
    55261 ttgcttatca gccatgactt acaaatgctt agccaatggg cggataaaat taacgtgctt
    55321 tactgcggtc aaacagtgga aaccgcgccg agtaaggagt tggtgacgat gccacatcat
    55381 ccttataccc aggcgctgat ccgcgcgata ccagacttcg gcagcgcgat gccgcataaa
    55441 agtcgcctca atacgctgcc cggcgctatc ccactgctgg aacagttacc gattggttgt
    55501 cgtctggggc cacgttgccc gtatgcacaa cgagaatgca ttgtgacgcc acgtttgacg
    55561 ggggcgaaaa atcatctcta tgcctgtcat ttcccgctga acatggagaa agagtgagat
    55621 gatcgaaacg ctgcttgaag tgcgtaattt aagtaaaacg ttccgctacc ggaccggctg
    55681 gtttcgtcgt cagaccgtag aagcggtaaa acccttgagc tttacgctac gtgaaggcca
    55741 gacactggcg attattggcg agaatggttc gggtaaatcc acgctggcaa aaatgctggc
    55801 gggaatgata gagcctacca gcggcgagtt attgattgac gatcatccac tgcattttgg
    55861 cgattattcc ttccgcagtc agcgtattcg catgattttt caggatcctt cgacctcgtt
    55921 gaatccccgt caacgtatct cgcaaattct ggattttcca ctgcgcctga acaccgacct
    55981 ggaaccggaa cagcgtcgca aacagattat tgaaacgatg cgtatggttg ggctgctacc
    56041 ggatcacgtc agttactatc cgcatatgtt ggcacccgga caaaaacagc gtctgggtct
    56101 ggcgcgcgcg ctgatattgc gcccaaaagt cattattgcc gatgaagccc tcgcttcact
    56161 ggatatgtcg atgcgttcgc agttgattaa tctgatgctg gaattacagg aaaaacaggg
    56221 catttcgtat atttatgtta cccagcatat cggaatgatg aagcacatta gcgaccaggt
    56281 gctggtgatg catcagggcg aggttgtcga gcgaggcagc accgcggatg tgctggcatc
    56341 gccgctgcat gaactcacca aacggctgat tgccgggcac tttggcgaag cattgacagc
    56401 ggatgcgtgg cgtaaagatc gctaattttc tgaatcctcc ttccagatta tccacctcat
    56461 tctgacaaga gattaaatct tgtcagaatg aggtgatgga taaaataaaa tcaaatgcgc
    56521 gtgatttaag acgcaatctc actttgcagg aacgaaagct ctggcgatat cttcgcagcc
    56581 gacgttttgg tgatttcaaa tttcgccgtc aacatccggt ggggagctac attctcgatt
    56641 ttgcttgctg ctcggcgcgc gtagtcgttg agctggatgg tgggcagcat gatttggcag
    56701 ttgcctatga taccaggcgc actagctggc ttgagtcgca gaactggacc gtgctgcgtt
    56761 tctggcataa cgagattgat tgtaatgagg aggcggtgct ggagattatt ctgcaggaac
    56821 tgaaccgccg gtcaccctca ccctgaaagg gcgagggggc cgaccgagcc gaatagctgt
    56881 tgtggtgaaa acatggagac agtgctggag aatattcggc aaggtctgaa ccatcccagc
    56941 catcgccata aaagggttag gggctgtatg agcctgtttg ttgctggggt aacaatattt
    57001 gcacaatatg gtcccctcgc ccctctgggg agagggttag ggtaagggga aaagcgccca
    57061 ccctgccgca gcctgctccg gtcggacctg gcaactatag ctactcacag ccaggttgat
    57121 tataataacc gtttatctgt tcgtactgtt tactaaaacg acgaatcgcc tgattttcag
    57181 gcacaacaag catcaacaat aaggattaaa gctatgggtt ttctttccgg taagcgcatt
    57241 ctggtaaccg gtgttgccag caaactatcc atcgcctacg gtatcgctca ggcgatgcac
    57301 cgcgaaggag ctgaactggc attcacctac cagaacgaca aactgaaagg ccgcgtagaa
    57361 gaatttgccg ctcaattggg ttctgacatc gttctgcagt gcgatgttgc agaagatgcc
    57421 agcatcgaca ccatgttcgc tgaactgggg aaagtttggc cgaaatttga cggtttcgta
    57481 cactctattg gttttgcacc tggcgatcag ctggatggtg actatgttaa cgccgttacc
    57541 cgtgaaggct tcaaaattgc ccacgacatc agctcctaca gcttcgttgc aatggcaaaa
    57601 gcttgccgct ccatgctgaa tccgggttct gccctgctga ccctttccta ccttggcgct
    57661 gagcgcgcta tcccgaacta caacgttatg ggtctggcaa aagcgtctct ggaagcgaac
    57721 gtgcgctata tggcgaacgc gatgggtccg gaaggtgtgc gtgttaacgc catctctgct
    57781 ggtccgatcc gtactctggc ggcctccggt atcaaagact tccgcaaaat gctggctcat
    57841 tgcgaagccg ttaccccgat tcgccgtacc gttactattg aagatgtggg taactccgcg
    57901 gcattcctgt gctccgatct ctctgccggt atctccggtg aagtggtcca cgttgacggc
    57961 ggtttcagca tcgctgcaat gaacgaactc gaattgaaat aatcattctg ttggtaaata
    58021 ttgggcggcg ttctgccgcc cgttatctct gttatacctt tctgatattt gttatcgccg
    58081 atccgtcttt ctccccttcc cgccttgcgt caggataacg atttccttta cgaccaagga
    58141 gcgcccatgg aacaacgcca catcaccggc aaaagccact ggtatcatga aacgcaatcc
    58201 agtactgcgg agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc
    58261 tttctgctcg acgtgatcct tgatgaagaa acgctggctc ccttcctttc atggctggtc
    58321 cctgcgcgcg ttcttgcagt ggaattgttc cctgaccagc ttaccgtgac ccgttcacag
    58381 accttcaccg cttatgaacg cttgtctacg gccctgacgg ttgctcaggt ttgcggcgtc
    58441 cagcggttat gtaactacta ttcggcgcga cttacgccgc tccccgggcc tgattcctcc
    58501 agggaaagca atcataggtt ggcacaaatc acgcaatatg cccgccaact ggctagctcg
    58561 ccttctatta tcgacaaccg atcgcgccag catctgaatg acgtcggtct tactgccggg
    58621 gactgtgtga tcattaacca aatcattggt tttattggct ttcaggcgcg gaccattgcg
    58681 acatttcagg cttatctcgg gcacccggta cgctggttac ccggtctgga aatacaaaac
    58741 tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag ctatgaggtg
    58801 gaaaaactac ctgaagagta cacaaaaagt tcaacagcag aactttgcca actggccgaa
    58861 acactctctc tccaccctat ttcactttcc cttctcgaaa ggttgttaaa cagtacacgg
    58921 gttaatacac agccggataa taagcttgcg gcgttgttat gcgcgcggat aaatggcagt
    58981 cctgcttgtt ttgccgcctg tatggattca tcaaatgaat ataaaaaaat cagccccctt
    59041 ctgcgcaagg gcgaaaatga aattaaccaa tgggctgacc gtcattctgt tgagcacgct
    59101 accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc cgcccagttc
    59161 agccctttac tcgaacacga aaaatcatca acgcagatta ttaatctgct ggtatggagc
    59221 gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc
    59281 cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa aatagacaat
    59341 tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt cccagacgcc
    59401 acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc
    59461 gcaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat
    59521 tatcgcggtg atccacagtg aaaaagaacg tgaatccgca gagccagaag aactggttga
    59581 accgttcctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc tggccatcgt
    59641 tcctgatcat ccactcttaa aagacgccat tccttgccgc gcagcccgtg gcctgaacca
    59701 cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga
    59761 tcgttctttc tatgctgaac tgacacaata catcactttt ggtgacgacc actttgtacc
    59821 gtggtgggtt acccttgcgc gccataatct ggaaaaagaa gcaccagacg gcgtcgctac
    59881 cgaaatgctc gatgaaggtc tggttcgtga agatctgacc gcgctggatt ttgtcaccat
    59941 cgacagtgcc agcacagaag atatggatga cgcccttttc gctaaggcgt tgccggatga
    60001 caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg aaggcagcaa
    60061 gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct tcaacatccc
    60121 tatgctgcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg aagtccgccc
    60181 ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata atatcgaatt
    60241 ctttgccgcc accatcgaat ccaaagcaaa gctggtgtat gaccaggttt ctgactggct
    60301 ggaaaatacc ggtgactggc agcctgaaag tgaagcaatt gccgaacaag tccgtttgct
    60361 agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga
    60421 tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga
    60481 gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta acatttgtgc
    60541 ggcccgcgta ctgcgcgata agctcggttt tggcatctat aacgtgcata tgggctttga
    60601 tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc
    60661 cgaagaagtg ctcacgctgg acggtttctg caaactgcgt cgtgaactgg acgcgcaacc
    60721 aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa ttagcactga
    60781 acccggtcct cactttggcc tcggtctgga agcatacgcc acctggactt cgccgatccg
    60841 taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag gcgaaactgc
    60901 gacgcgccca caggatgaaa tcactgtcca aatggccgag cgtcgccgtc tcaaccggat
    60961 ggcagaacgt gatgttggtg actggttata cgcacgcttc ctgaaagaca aagccgggac
    61021 cgacacccgt ttcgcagcgg aaattgtcga tatcagccgt ggcggcatgc gtgttcgttt
    61081 ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg tgcgcgatga
    61141 actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg tttacaaagt
    61201 gactgacgtt attgacgtca ctattgccga agtccgcatg gaaacccgca gcattattgc
    61261 gcgcccggtc gcgtaatctc ctttcacggc ccattcctca tggatgggcc gtttatttcc
    61321 ccgctctacc ttcgttatat ccaggcagat tttttaactg actttcgttt gaaaactggc
    61381 gtttttccaa aactggatta caaataaata tataaaaaaa acaatgcgtt tagccgccgt
    61441 taatttcatg taagtcatgt tgttcctttt atttaaccgc ggactccgca gttaaccgga
    61501 ggatatgcat catgaaaacc gttagggagt ccacaacgtt gtacaacttt ctcggatcgc
    61561 acaatccata ctggcggttg acggaaagca gcgatgtttt gcgcttttct accaccgaaa
    61621 ccacagaacc tgatcgtact ttgcagttat ctgccgaaca ggctgctcgc atcagggaaa
    61681 tgacggtcat cacctccagc ctgatgatga gtctgaccgt cgatgagagc gatctttctg
    61741 tgcatctggt aggacgaaaa atcaataaac gggaatgggc tggcaacgcg tctgcatggc
    61801 atgacacacc ggcggttgct cgtgatttat cacacgggct ttcctttgct gagcaggtag
    61861 tttctgaagc acattccgca atagtcattc tcgacagccg ggggaatatt caacgcttca
    61921 atcggttatg tgaagattac acagggttga aagaacacga cgtcattggg caaagcgtgt
    61981 ttaaactgtt tatgagccgt cgtgaagctg cggcatccag gcgcaataac cgtgtatttt
    62041 ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc aacatgtaaa ggccagcggc
    62101 tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg caaaaacgag atttttttaa
    62161 tctgttccgg caccgacatt accgaagagc gccgcgctca ggagcgactg cgtattctgg
    62221 caaataccga cagtatcacc ggactgccga atcgtaacgc aatgcaggat ttaatcgatc
    62281 acgctattaa tcatgcagat aacaataaag ttggggttgt gtatcttgat ttggataatt
    62341 tcaaaaaggt caatgacgcc tatgggcatt tgtttggtga ccagttatta cgcgacgtgt
    62401 cattggctat tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca ggtggggatg
    62461 agtttctggt actggcatcc aacacctcac aaagcgcgct ggaagcaatg gcatcacgaa
    62521 ttttgacccg cttacggctc ccctttcgca ttggtttaat tgaagtttat accagctgtt
    62581 cagtaggtat tgcactctct cccgaacatg gttcagacag cacggctatt attcgtcacg
    62641 ccgacacagc aatgtacaca gcgaaggaag gcggacgagg acaattttgc gtttttactc
    62701 cagaaatgaa tcagcgggta tttgaatatc tctggctgga taccaacttg cgtaaagcac
    62761 tggaaaacga tcagttggtt attcactatc aaccgaaaat cacctggcgt ggcgaagtgc
    62821 gcagtctgga agcactagta cgttggcagt cacctgaacg tgggttgatt ccaccgttgg
    62881 acttcatttc ctacgccgaa gagtcagggc taattgtgcc tttaggccgt tgggtgattc
    62941 tcgatgtcgt acgccaggtg gcaaagtggc gggataaagg cataaacctg cgagtggcgg
    63001 taaatatttc tgcacgtcag ctcgccgatc aaaccatttt caccgccctg aaacaggttc
    63061 tccaggaact caattttgaa tactgcccta tagatgttga actgacagag agttgtctga
    63121 ttgagaatga tgaactggca ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag
    63181 tgcatctgga tgattttggt accggctact cttcactttc gcaactggcg cgctttccga
    63241 tcgatgccat caaacttgac caggtttttg ttcgagatat tcacaaacaa cctgtctcgc
    63301 agtcactggt ccgggcgatc gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg
    63361 aaggtgtaga gagtgcaaag gaagatgctt ttttaaccaa gaacgggatc aatgagcggc
    63421 aaggattttt gtttgccaaa ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct
    63481 atctgaagcg cgcataagta gatgaatata tatacttagc gctatatccc gtcattatat
    63541 atcgtattcc tggatgataa ttagccgttg ctgttaaaca cttctctttt agcggcaatt
    63601 tttgcattat tcattttcca cttttgctat aaggatgtcc ttgatgtctg aatttgatgc
    63661 ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca
    63721 ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc
    63781 tacgccagac atcccgaagg caccgtggga gatttaacaa tcgtgccttc aggttttcct
    63841 atttgaggat gcccgg
//
LOCUS       DAZFWP020000006        61415 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFWP020000006 DAZFWP020000000
VERSION     DAZFWP020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 61415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.006"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            60..578
                     /locus_tag="ACHK0Z_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             60..578
                     /locus_tag="ACHK0Z_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4500422.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            594..1133
                     /locus_tag="ACHK0Z_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             594..1133
                     /locus_tag="ACHK0Z_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU4500423.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1228..2805)
                     /gene="guaA"
                     /locus_tag="ACHK0Z_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             complement(1228..2805)
                     /gene="guaA"
                     /locus_tag="ACHK0Z_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU4500424.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2874..4340)
                     /gene="guaB"
                     /locus_tag="ACHK0Z_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             complement(2874..4340)
                     /gene="guaB"
                     /locus_tag="ACHK0Z_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU4500425.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4502..5872
                     /gene="xseA"
                     /locus_tag="ACHK0Z_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             4502..5872
                     /gene="xseA"
                     /locus_tag="ACHK0Z_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU4500426.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5869..6084)
                     /locus_tag="ACHK0Z_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             complement(5869..6084)
                     /locus_tag="ACHK0Z_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4500427.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6153..7625)
                     /gene="der"
                     /locus_tag="ACHK0Z_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             complement(6153..7625)
                     /gene="der"
                     /locus_tag="ACHK0Z_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU4500428.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7743..8921)
                     /gene="bamB"
                     /locus_tag="ACHK0Z_RS01735"
                     /old_locus_tag="pgaptmp_000347"
     CDS             complement(7743..8921)
                     /gene="bamB"
                     /locus_tag="ACHK0Z_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU4500429.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8932..9552)
                     /gene="yfgM"
                     /locus_tag="ACHK0Z_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             complement(8932..9552)
                     /gene="yfgM"
                     /locus_tag="ACHK0Z_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU4500430.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9570..10844)
                     /gene="hisS"
                     /locus_tag="ACHK0Z_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             complement(9570..10844)
                     /gene="hisS"
                     /locus_tag="ACHK0Z_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU4500431.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10955..12073)
                     /gene="ispG"
                     /locus_tag="ACHK0Z_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /gene_synonym="gcpE"
     CDS             complement(10955..12073)
                     /gene="ispG"
                     /locus_tag="ACHK0Z_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU4500432.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12100..13113)
                     /gene="rodZ"
                     /locus_tag="ACHK0Z_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             complement(12100..13113)
                     /gene="rodZ"
                     /locus_tag="ACHK0Z_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU4500433.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13398..14552)
                     /locus_tag="ACHK0Z_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             complement(13398..14552)
                     /locus_tag="ACHK0Z_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU4500434.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14702..15133)
                     /gene="ndk"
                     /locus_tag="ACHK0Z_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             complement(14702..15133)
                     /gene="ndk"
                     /locus_tag="ACHK0Z_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU4500435.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15282..17594)
                     /gene="pbpC"
                     /locus_tag="ACHK0Z_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             complement(15282..17594)
                     /gene="pbpC"
                     /locus_tag="ACHK0Z_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU4500436.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPDQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17595..22556)
                     /gene="yfhM"
                     /locus_tag="ACHK0Z_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             complement(17595..22556)
                     /gene="yfhM"
                     /locus_tag="ACHK0Z_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU4500437.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLYLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22763..23608
                     /gene="sseA"
                     /locus_tag="ACHK0Z_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             22763..23608
                     /gene="sseA"
                     /locus_tag="ACHK0Z_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU4500438.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24270..24395
                     /gene="timP"
                     /locus_tag="ACHK0Z_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             24270..24395
                     /gene="timP"
                     /locus_tag="ACHK0Z_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU4500439.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24427..25203)
                     /gene="sseB"
                     /locus_tag="ACHK0Z_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             complement(24427..25203)
                     /gene="sseB"
                     /locus_tag="ACHK0Z_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU4500440.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25345..26628)
                     /gene="pepB"
                     /locus_tag="ACHK0Z_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             complement(25345..26628)
                     /gene="pepB"
                     /locus_tag="ACHK0Z_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU4500441.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26815..27006)
                     /gene="iscX"
                     /locus_tag="ACHK0Z_RS01800"
                     /old_locus_tag="pgaptmp_000360"
     CDS             complement(26815..27006)
                     /gene="iscX"
                     /locus_tag="ACHK0Z_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU4500442.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLD"
     gene            complement(27018..27353)
                     /gene="fdx"
                     /locus_tag="ACHK0Z_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             complement(27018..27353)
                     /gene="fdx"
                     /locus_tag="ACHK0Z_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU4500443.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27355..29205)
                     /gene="hscA"
                     /locus_tag="ACHK0Z_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             complement(27355..29205)
                     /gene="hscA"
                     /locus_tag="ACHK0Z_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU4500444.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29222..29737)
                     /gene="hscB"
                     /locus_tag="ACHK0Z_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             complement(29222..29737)
                     /gene="hscB"
                     /locus_tag="ACHK0Z_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU4500445.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29833..30156)
                     /gene="iscA"
                     /locus_tag="ACHK0Z_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             complement(29833..30156)
                     /gene="iscA"
                     /locus_tag="ACHK0Z_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU4500446.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30173..30559)
                     /gene="iscU"
                     /locus_tag="ACHK0Z_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             complement(30173..30559)
                     /gene="iscU"
                     /locus_tag="ACHK0Z_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU4500447.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30587..31801)
                     /gene="iscS"
                     /locus_tag="ACHK0Z_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             complement(30587..31801)
                     /gene="iscS"
                     /locus_tag="ACHK0Z_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU4500448.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31913..32401)
                     /gene="iscR"
                     /locus_tag="ACHK0Z_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             complement(31913..32401)
                     /gene="iscR"
                     /locus_tag="ACHK0Z_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU4500449.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32671..33411)
                     /gene="trmJ"
                     /locus_tag="ACHK0Z_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             complement(32671..33411)
                     /gene="trmJ"
                     /locus_tag="ACHK0Z_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU4500450.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33530..34333
                     /gene="suhB"
                     /locus_tag="ACHK0Z_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     CDS             33530..34333
                     /gene="suhB"
                     /locus_tag="ACHK0Z_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU4500451.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34478..35332
                     /locus_tag="ACHK0Z_RS01850"
                     /old_locus_tag="pgaptmp_000370"
     CDS             34478..35332
                     /locus_tag="ACHK0Z_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4500452.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35523..36803
                     /gene="csiE"
                     /locus_tag="ACHK0Z_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             35523..36803
                     /gene="csiE"
                     /locus_tag="ACHK0Z_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU4500453.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36795..37934)
                     /locus_tag="ACHK0Z_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             complement(36795..37934)
                     /locus_tag="ACHK0Z_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU4500454.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGESRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38094..38984)
                     /gene="hcaR"
                     /locus_tag="ACHK0Z_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             complement(38094..38984)
                     /gene="hcaR"
                     /locus_tag="ACHK0Z_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU4500455.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            39120..40481
                     /gene="hcaE"
                     /locus_tag="ACHK0Z_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             39120..40481
                     /gene="hcaE"
                     /locus_tag="ACHK0Z_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU4500456.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40478..40996
                     /gene="hcaF"
                     /locus_tag="ACHK0Z_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             40478..40996
                     /gene="hcaF"
                     /locus_tag="ACHK0Z_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU4500457.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40996..41316
                     /locus_tag="ACHK0Z_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             40996..41316
                     /locus_tag="ACHK0Z_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU4500458.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41313..42125
                     /gene="hcaB"
                     /locus_tag="ACHK0Z_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41313..42125
                     /gene="hcaB"
                     /locus_tag="ACHK0Z_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU4500459.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42135..43337
                     /gene="hcaD"
                     /locus_tag="ACHK0Z_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             42135..43337
                     /gene="hcaD"
                     /locus_tag="ACHK0Z_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU4500460.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43434..43856
                     /locus_tag="ACHK0Z_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /pseudo
     CDS             43434..43856
                     /locus_tag="ACHK0Z_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
     gene            complement(43904..44776)
                     /locus_tag="ACHK0Z_RS01900"
                     /old_locus_tag="pgaptmp_000380"
     CDS             complement(43904..44776)
                     /locus_tag="ACHK0Z_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4500461.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(44788..45882)
                     /locus_tag="ACHK0Z_RS01905"
                     /old_locus_tag="pgaptmp_000381"
     CDS             complement(44788..45882)
                     /locus_tag="ACHK0Z_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4500462.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45915..46913)
                     /locus_tag="ACHK0Z_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             complement(45915..46913)
                     /locus_tag="ACHK0Z_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4500463.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46938..48449)
                     /locus_tag="ACHK0Z_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             complement(46938..48449)
                     /locus_tag="ACHK0Z_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4500464.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48472..49455)
                     /locus_tag="ACHK0Z_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             complement(48472..49455)
                     /locus_tag="ACHK0Z_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4500465.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIY
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49552..52833)
                     /locus_tag="ACHK0Z_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     CDS             complement(49552..52833)
                     /locus_tag="ACHK0Z_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU4500466.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52951..54144
                     /locus_tag="ACHK0Z_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     CDS             52951..54144
                     /locus_tag="ACHK0Z_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4500467.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54208..55461)
                     /gene="glyA"
                     /locus_tag="ACHK0Z_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             complement(54208..55461)
                     /gene="glyA"
                     /locus_tag="ACHK0Z_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU4500468.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55789..56979
                     /gene="hmpA"
                     /locus_tag="ACHK0Z_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             55789..56979
                     /gene="hmpA"
                     /locus_tag="ACHK0Z_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU4500469.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57024..57362)
                     /gene="glnB"
                     /locus_tag="ACHK0Z_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             complement(57024..57362)
                     /gene="glnB"
                     /locus_tag="ACHK0Z_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU4500470.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57423..58757)
                     /gene="glrR"
                     /locus_tag="ACHK0Z_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             complement(57423..58757)
                     /gene="glrR"
                     /locus_tag="ACHK0Z_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU4500471.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58747..59460)
                     /gene="qseG"
                     /locus_tag="ACHK0Z_RS01955"
                     /old_locus_tag="pgaptmp_000391"
     CDS             complement(58747..59460)
                     /gene="qseG"
                     /locus_tag="ACHK0Z_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU4500472.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59625..61052)
                     /gene="qseE"
                     /locus_tag="ACHK0Z_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             complement(59625..61052)
                     /gene="qseE"
                     /locus_tag="ACHK0Z_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU4500473.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT        14556 a        16928 c        15419 g        14512 t
ORIGIN      
        1 ctttcctctt ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag
       61 tgatgaaatt taaaaaatgt cttctgcctg tagcaatgtt agcgtcattc actctggcag
      121 gatgccagtc aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata
      181 ccaaacaaga aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag
      241 acaactccca aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg
      301 gcggtgttat cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag
      361 ttggcggcgg agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg
      421 aaggtgtttc tttaacgtat aaggaaggca ccaaagtgta tacctccacc caggtgggta
      481 aagagtgcca gtttacgaca ggtttagccg ttgttattac cacgacgtat aacgaaacgc
      541 gtattcagcc aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga
      601 aagtttttct ttgcgccatc ttggcctcct taagctatcc ggctatcgcc tcatcattgc
      661 aggatcaact ctcggctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaagc
      721 agcatgacga atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg
      781 caaatgccca ggccgccgct aacaaaagag cggcaacggc agcagcaaat aagaaagctc
      841 gtcaggataa actggacgcc gaagcctctg cggacaaaaa acgcgatcaa agttatgaag
      901 atgagctacg cagcttagag attcagaaac aaaaactggc gctggcaaaa gaagaagccc
      961 gcgttaagcg agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg
     1021 atgtggtgca atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga
     1081 tgaaaagcgt gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt
     1141 tagtaacttc aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt
     1201 tgttcattca tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc
     1261 gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac
     1321 gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt cgatggtttc
     1381 gacagcacgc agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga
     1441 acgtaccggc aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg
     1501 cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac
     1561 ttcacccagc acacgaacgc caaggcctgg tcccgggaac gggtgacggt acagcatgtc
     1621 gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag
     1681 cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt
     1741 gatgacgtgt gctttaccgg ttgcagaagc cgcagattcg ataacgtcag ggtagatggt
     1801 gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac
     1861 gaaaacgcgc ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga
     1921 caggaagcga tcttctgccg gtacgtgaac aatgttaaga ccaaagtgat cgccaaacat
     1981 atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt
     2041 caggttttta ccgatagcgc ggtgcagcag cattgcggtt acggaggaat ccacaccacc
     2101 agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag ctacagcatc
     2161 gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg
     2221 ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata
     2281 gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt
     2341 gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag
     2401 cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc tgtcgtttac
     2461 gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa
     2521 ctgcattgcc atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa
     2581 gacatactgc ggcgcacgcg ggctgttttc ttcggtggtg ctttccgggc cgccggaaag
     2641 aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag
     2701 ttcgcagtaa acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc
     2761 gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga
     2821 ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag
     2881 cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga
     2941 atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca
     3001 cagccggtca gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc
     3061 aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg
     3121 ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata
     3181 ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca
     3241 gtacccgcca gcatggaacc taccatcacc gcgcttgcgc cagctgcgat agctttggcg
     3301 atgtcgccgg agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg
     3361 gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga
     3421 gtcgtacaga tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc
     3481 aaagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat
     3541 ttagcacggg tttcacggat acgctgcaaa actccttctg agtgaccgtg ggaggagtcg
     3601 atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca
     3661 cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt
     3721 ttacgttccg ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca
     3781 tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg
     3841 gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc
     3901 tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt
     3961 tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg
     4021 cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt
     4081 ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg
     4141 ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct
     4201 gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca
     4261 gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct
     4321 tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc
     4381 ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc
     4441 cccgaagggc gggttactat cgactgaata acctgctgat tttgaatttg atctcgctca
     4501 catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg
     4561 tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac
     4621 acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg
     4681 cgcgatgttc cgcaacagca accgtcgggt gaccttccgt ccacagcatg ggcaacaagt
     4741 tttagttcgc gccaatatta cgctctacga gccgcgcggc gactaccaga taatcgttga
     4801 gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa
     4861 gttgcaggct gaaggattgt tcgatctgca atacaaaaaa ccacttccct cccctgcgca
     4921 ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt
     4981 aaaacgtcgc gatccttctc taccggtgat catctacccc accgccgttc agggcgatga
     5041 cgcgccgggg caaactgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt
     5101 gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg
     5161 cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac
     5221 ggatgtgacc attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc
     5281 tgaagtagtg agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg
     5341 gctggagatg gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca
     5401 tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga
     5461 gcgcctgcaa aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca
     5521 gcagcagcgg ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc
     5581 gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag
     5641 cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc
     5701 aacgctggcg cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt
     5761 gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag
     5821 tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac
     5881 gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc
     5941 accgcaggct tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc
     6001 aataatttcc ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg
     6061 acattgtgga caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata
     6121 gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc
     6181 agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg
     6241 ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg
     6301 aagtagttca tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga
     6361 atcaccacaa tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga
     6421 ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata
     6481 gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta
     6541 ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc
     6601 agacggaagt ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac
     6661 ttattcacca caatgacaag tgaacgccca ctattgagaa taaagcccag cagcgagaga
     6721 tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggcatcttca
     6781 atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt
     6841 ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg
     6901 tagatactgt cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga
     6961 atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc
     7021 ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca
     7081 aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc
     7141 cacggcagca gcacatgctc cagcagactt aatacgccac gaccgtgaga cgcggcgatc
     7201 gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga
     7261 ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag atgtttggca
     7321 atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag tacgacgtcc
     7381 gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg
     7441 ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg
     7501 tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt
     7561 agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt
     7621 accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt
     7681 tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac
     7741 gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca
     7801 gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga
     7861 ccatcttcga cgttaatcca gtgcagataa ccttcactgt caccgaccac caggttgcca
     7921 ttatacagca ccggagaagt cagcaggcga tgcagcagat cgctttgtgc ccacagcgta
     7981 acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga
     8041 ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca
     8101 ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac aacgccgtta
     8161 acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc
     8221 tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga
     8281 ccattatcgc cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc
     8341 aaagagagcg aaggcatatc aaggttaact gtccatttga cagcgccgtc agcttcgttc
     8401 agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct gaccaccggg
     8461 cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc
     8521 agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca
     8581 ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca
     8641 gaccagattt ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct
     8701 gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg
     8761 ctaccaacgg aagtactcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat
     8821 ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta
     8881 acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga
     8941 caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac
     9001 gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca atgcttcacc
     9061 acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag
     9121 cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc
     9181 tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg cagctttctc
     9241 tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt
     9301 atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct
     9361 cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg
     9421 gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag
     9481 tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc
     9541 gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa gtgcgcggct
     9601 acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg
     9661 ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca
     9721 cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt
     9781 aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa
     9841 gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact
     9901 aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc acgaccgccc
     9961 agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgaga
    10021 ctgttagtca cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc
    10081 tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc
    10141 tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc
    10201 acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag
    10261 tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg
    10321 gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc ggaaataccc
    10381 agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc cggaccttgc
    10441 agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc
    10501 tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca
    10561 tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg
    10621 ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc
    10681 gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag
    10741 ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc
    10801 aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg
    10861 aatataaaaa tgaaccctca acgcttccct caatgtttcg ggagctatgc gggttcaatc
    10921 atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa
    10981 ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga
    11041 tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct
    11101 tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc
    11161 agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt
    11221 taaccgtacc gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttga
    11281 tcccgcgcga acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga
    11341 ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat gccttcagac agcagcagac
    11401 ctaaaccaat ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca
    11461 gatgcagcgg ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga
    11521 cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa
    11581 catggcgcat ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca
    11641 gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg
    11701 cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca
    11761 gacaatcgac gccgtattcc gctactttca gtgcaatgcg atagtcgaag tggatgtcag
    11821 ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg
    11881 tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga
    11941 ccgttgcttc gacgtctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac
    12001 catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag
    12061 cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc
    12121 ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg
    12181 tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt tgtatggtgc
    12241 ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca attttttacc
    12301 ggtagcatca gtaacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg
    12361 atcagccgcc ggcgtagtca cgcctgcctg atcggttggc aacggcgcag cgccatctgg
    12421 cgttgttgtt gccgttggtg ccggggtcgc ggcggtatca acatttgcct gcgaaggcga
    12481 aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg
    12541 cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt
    12601 agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc tcagttccgc
    12661 cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca
    12721 ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca
    12781 gccgtcacgt tttttgcggc gtttaccgag ggaaaaactc tgcataggcg caacttttgc
    12841 agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac
    12901 cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc
    12961 cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc
    13021 ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc
    13081 ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat
    13141 tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt
    13201 tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa
    13261 cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga
    13321 ccagacaatt gcaacttaat acttcattca taaaagatta cgccacggta cataaagtaa
    13381 ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta
    13441 cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg
    13501 atatcatcac cacgggtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt
    13561 gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcgcccgg gaaggggttc
    13621 cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg
    13681 tgttcagtgc cgtcgttaac gtggtcaagc atcacatact caatagtgac acggccctga
    13741 ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac
    13801 tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt
    13861 gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta
    13921 gaaagcgtga cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc
    13981 gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta
    14041 gtgattgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc acgccacacc
    14101 tggccaataa tttccgatac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag
    14161 aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct
    14221 tccgggatat acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg
    14281 ccgtcggatg aacgctgttc ttcaaccact tccggtgcgc ggatttccgc cacctctttc
    14341 agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag
    14401 tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa
    14461 aactcccgca tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg
    14521 acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt
    14581 tatggcccct ggaggattga aaaaagaaac gccccggtga gcggctgctc gtccgggggc
    14641 gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt
    14701 attaacgggt gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt tcgcgagcgg
    14761 cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag
    14821 cgcgcagagt accagccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac
    14881 gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt
    14941 caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgtgcctgtt
    15001 caacggtcag gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg
    15061 caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac
    15121 gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag
    15181 atggcgcgga ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt
    15241 aaaataaaat gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc
    15301 actgtcgcga tttgccccac atcatccatc accagcaatt gataatcacc tttatccgtc
    15361 aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag
    15421 cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa
    15481 cgtttaataa tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga
    15541 ccgtatgacg ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg
    15601 ggcagccagg gttccagcgg tagcggccag acatttatca tttcttgtcg cgcttgcggg
    15661 caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg aatgccatta
    15721 atgccttcct gatccggcag taatagagtt ggcggctgac tcccgtccag cagccaggtt
    15781 gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag
    15841 ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac
    15901 agtaagatat tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca
    15961 acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg agcgttaacc
    16021 ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg
    16081 acgcgcggca aggcactatc cggcaagggt ttcgcttcat cagccataat ccggcgaatg
    16141 atccacgccg cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag
    16201 cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc
    16261 agttttgcac cagcgccgcc gagaatgagt gaaagattcg gcgcagcacc gttgggcaaa
    16321 tataacggca atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc
    16381 agcacctgca cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg
    16441 ccatgaaaac cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct
    16501 tgcagcagtg atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt
    16561 ttgagcaccg atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa
    16621 tcatcgttga gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc
    16681 acgatcatcg ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct
    16741 tccagacgtc gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta
    16801 ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag
    16861 atgggttctt cccttgactc ttttacctgc tcacgggacc acacaccttg cacggccatc
    16921 cgttcgagca ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga
    16981 cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca
    17041 ggcgactttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac
    17101 ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc
    17161 aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga
    17221 cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct
    17281 tgccatgctg cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga
    17341 tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga
    17401 taacgccaga tgccgtcagc atcggcgaag cgccatagcg gcgtaccatc ctgcgccacg
    17461 accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac
    17521 gctgcaagaa aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt
    17581 aacaagcgag gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc
    17641 cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc
    17701 accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc
    17761 acaaagcgat cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt
    17821 aagttttgca cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctga
    17881 ttttccagtt ccagacccgc aggcagcaga tccacgacta acgcatccgg cacgctgtta
    17941 ctggctttta cctgcaacca gaccagcacc agatcgccgc tacgtaacga gtccagcgat
    18001 ttgctcttac catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt
    18061 aaaggtgcgg attgcggata accgctggca tccacacgca gccataacgg ctgatcgcca
    18121 ctgtttctca cctgcaaggt ggcaagttga tcgctattca gattgctgtt tagcgttttc
    18181 tcgcctgtca gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc
    18241 tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca
    18301 ccaaatgcct gctgcgaaag cgtgttcagc aaagtgttct gctcatcggg tagcagttta
    18361 ttttcttcca gcaagtagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc
    18421 agccatatcc gctcatcact atttctcggc gttttcagcg ccagcacaat cgcttcttca
    18481 ccacgcgttg catcacccat ggttttcagc gcaacgccaa gttgcagcag cggtaaacca
    18541 gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc
    18601 tgacgggcca gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta
    18661 tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta
    18721 ccccggttaa tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa
    18781 tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag
    18841 ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg
    18901 cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa
    18961 agtgacggga acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac
    19021 tctttgatat aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc
    19081 agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc
    19141 tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc
    19201 cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc
    19261 tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc
    19321 acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg
    19381 gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc
    19441 agacgcgagg tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt
    19501 gcggcaacta tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc
    19561 atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt
    19621 tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt
    19681 ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc
    19741 tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca
    19801 aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac accactatca
    19861 acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta
    19921 attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg
    19981 aggcgacggt tttcatcgcc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc
    20041 gcggagcgag atttatcgcc aggacgtacc accagcgtac ttaaatacag atcatgacga
    20101 ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct
    20161 tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc
    20221 gcggcgatat gcaacttaat ggtgtcgcca gggcgataac tggctttatc cagtttcagc
    20281 gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctgt tgtcctgcca gctatagcca
    20341 gcccagaaac gaacactact gaccgcttca ttcggcgctt tgacttccag acgataagcg
    20401 ccccactcta ccggaaaact gaccttgccg gtttcgtccg ctttcagatc cagagtttgt
    20461 tcattttcga tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag
    20521 ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt
    20581 ttcacgcctt gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca
    20641 atgggctgtt tcaccgtgct gtcagtacga taatcgtaaa ccgatttcga ggcgaactgc
    20701 ggacggatcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc
    20761 gggcgaccgc ccgattccag caaactaccc tggaaaataa cctgtaatgg ggaatgcgtt
    20821 tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga
    20881 acttcatcca gcgtgcggga aagattttcg gcagctatat cgccgaattc aaaaccaggt
    20941 aaggctgaca cagcttcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca
    21001 ttagcaggtg caccatacag gtagtacccc accacggaga atttcacttc atctttcggc
    21061 gttagcgggg ttttctcacc ggtcagattc agcgccatgc gctctggcat aaaatcttcg
    21121 acgtggaaat cccacatccg atactggtta tcgcccgtgt tagcgcgaat atgccacata
    21181 ccggttgccg cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga
    21241 ctaacgacgc tcctgagtac ctgcccatcg ggtttaatca cgtctaactt gatgggttga
    21301 ttgggcaacg ctttaccgtc tgcatcacgc agcaaaccat tgaggattac cgtttcacct
    21361 gggcgataaa gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca
    21421 gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga
    21481 ccgtctttac gcgccaacag taatgccgcg tttttatcat tttccagctg cacatgcccc
    21541 tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa agagacttca
    21601 attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga
    21661 taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa
    21721 tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg
    21781 atatcgccca gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac
    21841 cgtccggtgt agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc
    21901 gaattgcggt attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag
    21961 aagttaacat cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg
    22021 ccaggcagca gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt
    22081 atagtttttt cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca
    22141 atagtaacga tcaaatcacg tttcggttcg aggtggcgta aacgtaactc tttaagatta
    22201 tctgacagct cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg
    22261 cgtgagaaat cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca
    22321 ccatcaagtt ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg
    22381 gccagttttt gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc
    22441 gctttagtaa cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg
    22501 tcgcaccctg ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt
    22561 catccctgcc acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg
    22621 ttatgtaatt cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac
    22681 agccaaatca gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac
    22741 cccggcaaaa atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc
    22801 cgaacatatt gatgacccgg aaattcagat tattgatgcc cgcatggcgt cgcctggaca
    22861 ggaagatcgt aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt
    22921 tgatatcgaa gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga
    22981 aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta
    23041 tgacgaaggt aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt
    23101 agagaaagtg tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt
    23161 agaagaaggt gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc
    23221 cgtggtgaaa gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga
    23281 tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg
    23341 cggacatatt cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact
    23401 aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc
    23461 aattatcgtc agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac
    23521 gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc
    23581 agatttaccg gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt
    23641 tggcggtttt ttattgctgg tctggttcgc ggcctttcca gcaggttgac ttgtgttaca
    23701 tgagcaacgc aggtgcttca cagcaaaaca atactcacca gtaactctct ttttgtcaag
    23761 caaaagagag taattattgt ttatttagcg tattatcgac accggccctt tccgccgtgt
    23821 tcggtaataa attaacctgg cttattagtc cgaattcaga caaatataaa caaatcctgc
    23881 tcaaaattaa aaatactaac cggtaaaaga tattacttaa acatgtaaat tcactttcct
    23941 ttaaaaaaca aaaaaccgcc aaaatcaggc ggttatttgt tgctggtccg gttcgcggcc
    24001 tttccagcag gttgtattac cgtagtaatg caagcgcgtc tcagcggaga caatactcgc
    24061 cagtaactct ctttttgtca agcaaaagag agttattatt gttctgttag tgtattatcc
    24121 actgcggccc tttccgccgt ctcgcaagcg ggcgctggct taaggaaagg atgttccgcg
    24181 accgttagag caagcgcgtc tcagcgcttg ctatcgcggc aatatcgcca gtggtgctgt
    24241 cgtgatgcgg tcttcgcatg gaccgcacaa tgaagatacg gtgcttctgt atcgtactta
    24301 ttgtttctgg tgcgctgttt tcccaagtaa ataataaccg gagtctctcc ggcgacaatt
    24361 tactggtggt taacaacctt cagagcagca agtaagcccg aatgccgccc tttagggcgg
    24421 catattttag attatccggt tctgtttaaa gtcacgcaaa aaaccacccc agcgacgttc
    24481 atagaatggc gcaatatgtt cggtaataaa gtggctaatt cctttttccc cttttttcac
    24541 ctgacaaata tcgattggtt catcgccagg taatgtatcg gtcgctacac ttcccgtcgc
    24601 ctgaataatt tcttcgatat caccatcggc ttcaatgcca ataagtaaat taggctgtgc
    24661 ctcttcgttc tctttaattg aacaaataaa agcacgcttc accggcttaa tggttttaaa
    24721 taaggtggtg agtgaatcaa tcatttgtgc tggcggctct gcgacttccg ataatatcag
    24781 cgattcaccg ccttccagga tttcctggct gctcagcgga tttccctctc caccaatcaa
    24841 caaactgatt tcacgcggca taaattcttt accggtcggc agtttggcat taaggaagag
    24901 cgtttcgcca agggtcatct caaacaatgt gcgaacgggc attacgacaa atgcctgttc
    24961 gtcttcaacc gcctgttgaa gcgcttctaa cgaggtgaaa aaaggaatga cgctggtgcc
    25021 gtcttctttt tcccagtgct gtaaatcaag cgcactatct tcaaccacag cctcgccctg
    25081 cgccgccgta ccaggcaccc agacggtgga ttccagtaga gtacggaaaa aggccgggcg
    25141 gtgcgccggt tcggttgctg ctttttccag caggtcttca agttcgtttt ttgtttcgga
    25201 cataagaacc acaattcatt caacgttcgg ggcgcaaatg cctgatgcgc tacgcttatc
    25261 aggcctacaa gggattcgca atttgttgaa tttgcagaat ttgtaggccg gataaggcat
    25321 ttacgccgca tccggcatat tagtttacgc cgttaacaga ttagcaatcg tgcgcacacc
    25381 aagtcccgta gcacccgcag accactgttc aaccggcgct ttacggtaag tcgccgagca
    25441 gtcgatatgc agccagcctt gctgatagtt ctcaacaaag tgcgacagga agcccgccgc
    25501 cgtgctcgcg cctgccggat acgccgcgct tccggtattg ttcagttcgg caaagttaga
    25561 cggcagctgg ctgcggtgga attccgccag cggcagacgc cagaatggtt cgttctcttg
    25621 tgaggcactc gccagcaaac gaccggcaag cgcatcgtca aaactgaaca gcgcgtgata
    25681 atcattaccc agcgcagttt tcgccgcccc ggtgagggtc gccgcatcaa tgatcagttc
    25741 cggtttctgc gcactggcat caatcagacc atcggcaagc accagacgcc cttcggcatc
    25801 agtgttcatc acttcaactt ttttaccgtt gcgataggtg atgatatcgc ccagcttgaa
    25861 cgcattgccg ctaatcaggt tatccgcaca gcagaggaac agcttcacgc gcttgttcag
    25921 tccgcgcgta atggcaaatg ccagcgcccc ggtaaccgtt gccgcgccgc ccatgtccga
    25981 cttcatcgag tccataaacg cagtctgttt gatgctgtag ccgccggagt caaaagtgat
    26041 acctttacct accaggcacg cgtacactgg cgcttcttta tcgccagttg ggttgtaatc
    26101 cagcgccagc aataccggag aacgttctga accgcgtccg acggtgtgca gccccatata
    26161 accttgctca cgcagatctt cgcctttggt gatccgataa gtcacacgat cgcccgcgac
    26221 gttgctgatc agatcaacag cacgctgtgc cagttgcgat ggtcccaatt cttctgccgg
    26281 tgcgttgatg gtgtcacgca cccagtcgat gatcatcagg cggttatcca gttcctggcg
    26341 ctgggcatcg tccagatccg gccactccac tttacgcgtg cctttcgggg ctttgtaacc
    26401 ttgccagaat gcccagcagc gatccgcatc ccagccttca ccgcttaact gaacatgctt
    26461 gatgcccaga ccgtcaatct tgcgcgccgc acgctggatc agccccagat cgtctgcccc
    26521 gttcaaatgc agggtaatgc cgtcattatt aatgctgtaa gttgcttttt ctccccagcg
    26581 cgcgtcggca ggttgggtag agagggtaat cttcatcgct tctgtcattt tagttatcct
    26641 tctttgtaaa agggccgcca gttggcagcc ccaggatttt tccgctaaat gattcgcgtt
    26701 gcaggaaggc ggcaagtgag tgaagcccca ggagcataga taactatgtg actggggtga
    26761 acgagcgcag ccaacgcatc tgcggcgtga agcatgacgc ggaaattatt cggcctagtc
    26821 cagccagact aacaaaatcg cttcgaggat tttctcgttg gatgcctgcg ggtcgtcgtc
    26881 gaaatcttcc agatcgcaaa tccactgatg catatcggtg aatcgaaccg ttttcggatc
    26941 aagatcggga tacgcatcgt acagtgcttc gccaatttcg cggctatcgg tccatttaag
    27001 tcccatacta acctctgtta atgctcacgc gcatggttga tagtgtaacg cgggatttcg
    27061 accactaaat cttcgtcggt gacgcgcgcc tggcagctta aacggctttc cggctccagt
    27121 ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc tttccggcag cgagtcaaaa
    27181 ccttcacgaa cgatgcagtg gcaggtggtg caagcacagg atttttcaca ggcgtgttca
    27241 atctcgatac cgttacgcag agctgcgtcg agaatggttt caccgctatt agcttccaga
    27301 acagcgccat cagggcagag atcctgatga ggcaaaataa caatctttgg catattaaac
    27361 ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac tggtccatgc ggcgagcggc
    27421 gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt tcgatggcgt caacgtcatc
    27481 gccctgcgcc acttcactca ggtgagcggc agcatcgtca atgacctgac gttctgcggc
    27541 gcttaacagc gcggcatcag cagccagcgc gccgtgcaga ctttccagca cacgcgccgc
    27601 ttctactttt tgttctgcca gcattcgggc ttttacgtcc tgctcggcat agctcattga
    27661 gtctttgatc atcgaagcga tttcgctatc ggtcagaccg taagacggtt tgacctgaat
    27721 agacgcctca acgccggtgg atttctccat cgccgtcacg ctcaaaagac cgtcggcatc
    27781 gacctggaac gtcacgcgaa tatgcgcacc accagccggt agcgccggaa taccacgcag
    27841 cgcaaaacgc gccagtgagc ggcagtcctg caccagttcg cgctcaccct gcattacatg
    27901 gatagacatc gccgtctgac catctttaaa ggtggtgaaa tcctgagcgc gggccaccgg
    27961 aatagtggta ttacgcggaa tcactttctc caccaggccg cccatcgttt cgaggcccag
    28021 cgacagtggg atcacatcaa gcagcagcat ttcgctgtct ggcttgttac ccaccagaat
    28081 atccgcctga atcgccgcgc caatagcgac gactttatcc gggtcgatgg aagtcagagg
    28141 tggacgaccg aaaaattcgc ctacccgttc acgcaccagc ggcacgcgag tagaaccgcc
    28201 caccatcacc acttccagca cttcatcagc ttctacaccc gcgtctttca gcgcgcgacg
    28261 acaagccagt aaggttcgtt ttaccagtgg cgcgatcagt tcattgaatt gttcacggct
    28321 gatttcgccc tgccagcccg caacgttaac ggtcacggag tccgcatcgc tcagcgcgat
    28381 tttggctgca atggcggcat ccagcagttc acgctgaacg cggttatcgc tacgatcagg
    28441 aatgcccgcc tgctcgcgaa tgtaatccgc cagcagatgg tcgaaatcat cgccgccgag
    28501 cgcggaatca ccgccggttg ccagcacttc aaacacgccg cgacttaagc gcagaatgga
    28561 aatatcaaac gtcccgccac cgaggtcata aacagcgatc acgccttcct gaccggaatc
    28621 cagcccgtag gcgatagccg cagcggtcgg ttcgttaagt aagcgcagaa catgcaaccc
    28681 cgccagacgc gccgcgtctt tggtgccctg acgctgggca tcgtcaaagt acgccggaac
    28741 ggtgataact acaccatcca gctcgcctgc cagggcttca gttgcccgcg ccgccagtgc
    28801 tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc cccgccgccg tttcaatcat
    28861 cggcaggccg ttttcgctgg cctggaattg ataaggcaga tgcggatagc gttgctggat
    28921 atcagccagc gagcgcccca tcaggcgttt aacagaacta atggtgttgg cggtatcaag
    28981 cgctgcgtta gtacgcgcgt cataacccac cgaatgccct tgctgttgat agtgaacaac
    29041 agatggcagc aggtgacggc cttcatggtc ggctaacgtt tcggcctgac cgctgcgcac
    29101 tgtcgccacc agcgagttgg ttgtgcccag gtcaatacca gccgccagac gacgctgatg
    29161 cggcgcagca ctcaaaccag gttcactaat ttgtaataag gccatgttta gcttccagaa
    29221 attaaaaatc gagcagtttt tcttcgagtt gttcggcact gctccgcagt ttatcgagaa
    29281 aacgcagctt acgcacggta tccgccgccg cgtcccacgc ctcgttgtct aactgttcaa
    29341 ccatcaactg atggcgggta tcaaacatct ttttcacacg tttgataaag ctttccagcc
    29401 gcgcttcatc tttcgcctgt tcgatctcgt ccagctcttc gcgcagctcc aactgttcca
    29461 tcaggaacgc ggtgtcgcgc acagtatgct gctcgctggc gagatcaaaa ccgtgtaaag
    29521 aaagcaaata ttccgcgcgc attaacggat gacgcagcgt ttgccaggcc tggttaatgg
    29581 ttgcagattg ctgtacggcg gcgagttgtt ccgcctggct tccgctggcg aatttatcag
    29641 gatgatactg acgttgtaga tcctgaaaac gcaggctcag cgcctgggta tcgagttgat
    29701 aacgggcagg caagccaaag agggtgaagt aatccataac attctcaggg ctgcgatccg
    29761 ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc aggcgaccac ggtggggtta
    29821 tcggtatgcg catcaaacgt ggaagctttc gccgcaacca cactcatctt taacgttcgg
    29881 gttggtgaat ttaaaccctt cattcaggcc ttcttttacg aagtccagct gcgtaccgtc
    29941 cagaaattgc aggcttttgc catcgaccac gactttcacg cctttgtctt caaacacgat
    30001 gtcttccggc gtcggttcgt caacaaattc cagtacataa gccatacctg aacatccgga
    30061 ggttctcacg cccagacgca ggccaaaccc tttgccgcgg ttagccagaa aggtatttac
    30121 tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc aaacctcaac tcttattttg
    30181 cttcacgttt gcttttatag tccgcaatgg cggctttgat cgcgtcttct gccagaatag
    30241 aacagtgaat tttcaccggc ggcagttcaa gttcttcagc aatatcggtg tttttgatcg
    30301 cctgcgcttc gtcgagagac ttccctttca cccattcggt gaccagggag ctggaagcga
    30361 tagcagaacc gcagccgtaa gttttaaaac gcgcgtcttc aatgatacct tcatcgttga
    30421 ctttaatctg caacttcatc acgtcgccac aggccggtgc ccccaccatg ccgctgccga
    30481 cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg attctcgtaa tggtcgataa
    30541 ctttttcgct gtaagccatt ataaattctc ctgattccga taccgattaa tgatgagccc
    30601 attcgatgct gttcagatcc acgccctgct tgtacatttc ccacagcgga gaaaggtcac
    30661 gcagacgacc gatggattta cgaactaact cgatggtgta gtcgatctct tcttcagtag
    30721 taaaacgacc taaagagaaa cggatagagc tatgtgccag ctcgtcgttc agccccagcg
    30781 cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca ggcggaacct gaagaaactg
    30841 cgaggtcttt cagcgccata atcagcgact caccttcaac gtagttgaag ctgacgttga
    30901 gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta aacttcttcg atatctttga
    30961 tgccgttcca cagacggtta cgcaggccgc gcagacgttc catctcggtc gccatctctt
    31021 cttttgcgat gcgataggcc tcgcccattc cgacgatctg gtgaacaggc agagtgccgg
    31081 aacgcatacc gcgctcgtga ccgccgccgt gcatttgcgc ttcgatgcgt acgcgcggtt
    31141 tacgacgtac atacagcgca ccgatacctt tcgggccata gattttgtga ccggagaaag
    31201 acatcaggtc aactttcaac tggctcaggt cgataggcag tttacccacg ctctgggttg
    31261 catcaacgtg atagataatg ccacgagcac ggcacatttc gccgatagcc gcgatatcct
    31321 gcaccacgcc gatttcgtta tttacgtgca tgatggaaac aaggatggtg tcgtcacgca
    31381 tcgctgcttc aagttctttc aggtcgataa tgccgttacg ctgcggtgcc aggtaggtga
    31441 cttcaaaacc ttcgcgctcc agctgacggc aggtatccag taccgctttg tgttcggttt
    31501 tgctggtgat gatgtgcttg ccttttttct gataaaagtt ggctgcacct ttgatcgcca
    31561 ggttgtcaga ttcggttgca ccagaggtaa agacgatttc acgcggatca gcgccgacca
    31621 gatcggcaat ctgattacgg gcgatatcta ccgcttcttc agcctgccag ccgaaacggt
    31681 gagaacgaga ggccgggtta ccaaaggttc cgtccatcgt catgaactgc atcattttct
    31741 cggcaacacg cgggtccacc ggcgtggttg cggagtagtc gagataaatc ggtaatttca
    31801 ttgctctata aactccgtac atcactcaat gcaaggaatc aggctaccgg ctggatgtac
    31861 gaccgtgttt acggagtatt tagcactccg gcctgattct gaattctttt tattaagcgc
    31921 gtaacttaac gtcgatcgcg tcttgtgtgc gggtgcgtgg cgcgtcgtga gtatgctgac
    31981 gaccagacac atccagcact tcctggttat taaccagttc gcctaaagta atgttgttga
    32041 gaaaaccggt gagacggtcg ctcaaatcac gccacagcgc gtgggtcagg catttatcgc
    32101 cgccctggca gccgccttta ccctgacaac gggtggcatc tacagattcg tcaacggcgc
    32161 taatcacttc gccaacggcg atgctgctgg catctttgcc taacagataa ccaccgcctg
    32221 gtccacgtac gctggaaacc agaccatttt tacgcagacg ggaaaacagt tgttccagat
    32281 aagaaaggga aattccctga cgttcggaaa tatcagccaa cggtaccggg cccgcttcag
    32341 agttgagcgc aacgtcaagc attgcggtca cggcatagcg ccctttagat gtcagtctca
    32401 tgtcttactt cacctcaaac tcgcccctgc ccggggtttt ttattgtaaa agtgggggta
    32461 ttgcatagca gggtcaagtc tgacattccc gagtaaattg gtcaactatt tacttgactg
    32521 atttactcgg gtatttaacc ttcagtgcca tttttttatc gtggcgtttg tacctgttgt
    32581 cggatgcggt gtgaacacct tatccgacct acggttctgt acattgtagg cctgataaga
    32641 cgcgttaagc gtcgcatcag gcaactactg ttattcggcc ttgttacctt tattctgctg
    32701 ctcaatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    32761 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    32821 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    32881 atacggcgtc tcttcatgct cgacctgctc gccgttttcc tgagttgcca gccaggccat
    32941 gcgcacttca taggcaataa cctgaaccgc catcgccagg ttcagcgagc tgtattccgg
    33001 gttagccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    33061 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    33121 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    33181 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    33241 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    33301 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    33361 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    33421 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    33481 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    33541 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    33601 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    33661 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttacccac agcacaccat
    33721 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    33781 tccactggat ggtactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    33841 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    33901 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    33961 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    34021 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    34081 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    34141 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcggg
    34201 cggcatcgtc agcgacttca ccggtggtca taactatatg ctgaccggta acatcgttgc
    34261 tggtaaccca cgtgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    34321 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    34381 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    34441 cgattaatgg atgataaaga agtcgcaagg aaaggttatg gcactgccag tgaacaaacg
    34501 cgttcccaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    34561 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    34621 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    34681 ttcttcgacg ggccctgctg acaacgccat cgcaaccatc attcatgctc acggcaatgc
    34741 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    34801 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    34861 gctggacgat acgcaaagcg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    34921 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    34981 gggtgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    35041 catcgccaac cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    35101 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    35161 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagagc caaaacggtt
    35221 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgccacg gcgatgttta
    35281 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    35341 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    35401 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    35461 tcgtcatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    35521 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    35581 tcttgctgac gctctttcag ccggggttga ccgccaccac ggcaactttc agcgagctta
    35641 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacag gagatcctgc
    35701 gctatcatca actcacactg acggctggtt atgacggtag ctaccgggtt gaaggtacag
    35761 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccat
    35821 catttattac cagccatttc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    35881 cgcgtaactt ttatgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    35941 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    36001 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    36061 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    36121 tccagcctgt accacctgat gagccactgt ttatggcact acttttttcc atgttgcggg
    36181 ttcccgatcc actgcgggat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    36241 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    36301 gtgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    36361 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    36421 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagtg
    36481 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    36541 agattattct tcttaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    36601 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    36661 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    36721 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    36781 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    36841 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    36901 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    36961 cggatgactt cgctaccctg gcgagcagca atataacgca tggcggccag gtggcagacc
    37021 gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc acggcaacgc cgtagttgct
    37081 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgtgca
    37141 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    37201 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    37261 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    37321 cagttctggc gaaccagcgc caaccacgct gaccaaccgg tgctctcctg ctggcggctt
    37381 tccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    37441 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    37501 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    37561 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    37621 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    37681 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    37741 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    37801 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    37861 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    37921 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    37981 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    38041 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    38101 gttacgtttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    38161 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    38221 aaaacaacct gtccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    38281 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    38341 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    38401 ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc tttttcatgt
    38461 gctaaagggt gatcaaccgg taacacaacc actaatggtt cgtcaaaaag ctccagataa
    38521 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    38581 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    38641 ctgagacgaa acatcggtaa tacttttggc agtaaattca cttccgccga tggcacaaag
    38701 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    38761 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    38821 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    38881 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    38941 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    39001 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccagca
    39061 acatccttat ggcacaaaaa tagaaggtca atacatctga tctttcagga ttaaaaaata
    39121 tgaccacacc ctcagatttg aacatttacc aactgattga tacccaaaat ggtcgggtca
    39181 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    39241 gttgctggct atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    39301 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc atcaaggcgt
    39361 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    39421 gcgcctttac ctgcccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    39481 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    39541 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    39601 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    39661 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    39721 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    39781 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    39841 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    39901 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    39961 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    40021 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctaaac ggcactgcca
    40081 cactccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    40141 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    40201 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    40261 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    40321 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    40381 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    40441 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    40501 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    40561 atttcgtgac tggctggcgc agctcgacga agagattcgt tacaccatgc gcaccacagt
    40621 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    40681 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    40741 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    40801 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    40861 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    40921 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    40981 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    41041 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    41101 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    41161 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    41221 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    41281 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    41341 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    41401 gcgcgcaggt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    41461 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    41521 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    41581 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    41641 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    41701 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc tggtatcctg
    41761 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    41821 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    41881 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    41941 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    42001 cgggtccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    42061 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    42121 tttaaggaag cacgatgaaa gaaaaaacca tcattattgt cggtggcggg caagcggcgg
    42181 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    42241 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    42301 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    42361 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    42421 gcgaaagctg gcactgggat cagcttttta tagcaaccgg cgcggcagct cgaccgctgc
    42481 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    42541 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    42601 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    42661 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    42721 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    42781 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    42841 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    42901 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    42961 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    43021 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    43081 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    43141 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    43201 aaaacggcgt gcttatcggt gcagtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    43261 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    43321 cgcttaaatc actgtaacca ggataattag cgaatatctc aatgcctggg gcgtggcgag
    43381 gtgcaagagt gtgtattacg tttaaatcac ataatcttgc aaagggattg gttatgaaca
    43441 cactacgtta ttttgatttt ggagctgccc gccccgtttt gttattaatt gcccgtatcg
    43501 ccgtggtctt aattttcatt atttttggtt ttcccaaaat gatgggcttt gacggtacgg
    43561 tccaatatat ggcctcgttg ggcgcgccga tgccgatgct ggcagcgatt attgcggtag
    43621 ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt ccgctggcgg
    43681 tgctgtttat tttctacacg ctgggtacgg cggtgattgg tcaccattac taggatatga
    43741 ccggcgatgc ggttgggcca aatatgatta atttctggaa gaatgtcagt atcgctggcg
    43801 cattcctgct gttggcaatt accggaccgg gggcgatttc tctcgatcgg cgttaggaaa
    43861 gatgccggat gcggcatgaa cgccttatcc ggcattttat aaattacagc aacgcaaccc
    43921 gcaacgacat ctctgaagtt gttgattcac ctggtgcaag cgcaatgaga tcaccccctt
    43981 ccgggcgatg atgatcatcc ggcgcatggc tcatcggttc cagacagaag aaatcaaacg
    44041 catatccttt atcaaacgca gggtcggaaa caaagatgaa ataacacggt gcaggtggcg
    44101 tcgtttccat gatgatggca taaccttcct gcggctgctc gatgcgggcc tgaccattcc
    44161 atccggcaaa accattgttc acccactggc gcggcaacgg cgcgggctgg ttaaaatcca
    44221 gttcctgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgc tccagccagt
    44281 aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa cggaaaataa ggatgccagc
    44341 cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc accgtcagcg
    44401 tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg ctgcgatgtt
    44461 catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg cccagccagc
    44521 catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga tactcacgcc
    44581 cctgccagac aaaacgattg ccgctcaccc ggtttgcaaa tggcaccagc ggaaaacatg
    44641 aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc gtatcgcgcc
    44701 agaagccttc gattacgccg ccctggtcag aaacgtccag ctttagcgac ccgtgggata
    44761 aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa ctttcccgca
    44821 tttgccgctc gccatcagcg cataggcatc acctgcctgt tccagcgaga agcgatgggt
    44881 aatggcgtta cgcggccaca gcttccagtc cgtcagatca tgggcgcatt tttccatatg
    44941 gaacagactg gtcacccagg agccgataat ccgccgttga tggtgcatca gatcggcgct
    45001 gacctcgaat tccacttttc cggtttcacc aatgtaaacc acccgtcccc agtcagcggt
    45061 ggattgcagt gccagcaagc gacctgcggc attaccggaa caatcgagcg caacatccgc
    45121 gccaccgtgg gtgagttcgg cgataatctg cggcagacct tcggtggttg ctaaatagcc
    45181 gtgatccatc acccctaact gttttgccat cgccagacgt tccggcagca tatcaacgcc
    45241 gatgatccgt tttgcaccgc gacctttcgc cagcatcatc gccatcatgc cgaccggccc
    45301 cagaccgacc accagcacgt tatcgctgcc ggaaacttcg ccgcgcaaaa tcccttcata
    45361 ggccgtgcca acgccgcaac tgataaacgc accatcttcg tagctcagcg catccggcag
    45421 gaggatcaga tctttttctt ccgccagcag atattcggca tgaccgccgt cacgctgcca
    45481 gccgtaagcc gcttttcctt tgccagtaca agagatagga aagccgcggc ggcagttcgg
    45541 gcaaaaacca cagccagaaa tgtgatacac cagcacgcga tcaccctctt taaaatggcg
    45601 acagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga taaatccctg
    45661 gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt tgatgataga tatagtggac
    45721 atcgcttccg caaatcccgg aggatttcat tttgatcagt acctggttaa tccccggcgt
    45781 cggcaccgca acttcccgca gatcgacggt tgaatttcct ggtaaataag ctgccagcat
    45841 cgttttcata aatccctcat taacaatacg attaattttc atccctgccg cacccgcgcc
    45901 aggggcgtta aggtttagcg tttcgcttta ctgcttcgct gggtcagcaa gatattcgcc
    45961 agcaccgcca ccacgatgat gacgccgcgt accacctgct ggaaaaagga gttaataccg
    46021 agcagcacca gaccgttacc gattagcgta atcaccagca caccaagcaa tgtaccgaac
    46081 agggaaccgc gaccgccgga aagtgccgta ccgccgacca cgaccgcggc gatgacgtca
    46141 aactccagac cgtttgcggc acctgcgtta ccagaaccga ggcgcgccgc caacaaaatg
    46201 ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgcac ccgacgaacg
    46261 ttgatgccgc acaactgcgc cgccgtagca ttaccgccaa cggcaaaaac cgagcgcccg
    46321 aaggcggttt tgcggctgat gaacacaaac agcgcaaaca acacaatcat gatcagcgcg
    46381 gataccggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt ttcgtcaatc
    46441 ggcactggca gtgcgttcgt cataaacagc cccattccgc gcagggcgct ccacagcccc
    46501 agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca gcaccccggc cagcgttccc
    46561 atcagcgcgc ctaacagcaa caccagcaga cacgccaccg ccagcggaac ttcaaattgc
    46621 agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg gcccaacgct gacatcaatt
    46681 tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt ggcggcatcg
    46741 cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcattcag ggagaagacc
    46801 agataaagaa tggcgattac caccagcaaa ccgatctcat taatatggcg actgacaaat
    46861 tgtttgagcg agacgctctt gccctgcggc aatgataatg acgaagcaga catcttcagg
    46921 ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc atccacattg
    46981 accggcgagt gaaactcctg cgagaacgta ccgtgctgta acaacaggat gcggtcgcac
    47041 accagcggca actcctccac ttcactggag ataaacacca cgctttttcc ttcggcagcc
    47101 agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc gcgcgttggc
    47161 tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac cactttttgc
    47221 tgattgccac cagaaagcgt gccgatgggc gtttcgctac tggcggcctt gaccgtcatc
    47281 cgctgcatca cctcttcggt caggcggcgg atggtggacc attgcagcac tccgttggcg
    47341 ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca gggaatgatc
    47401 cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat gtcgccgtaa
    47461 tcggggcgcg tgattttctc gccgttgata acaatttcgc cctgttcata cgtctccagc
    47521 ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcaggccagc aatgccaagc
    47581 acttcgccgc gacgtaacgt aaagctgata tcctccagct tgggcttatg gcgtaacgca
    47641 cggacttcca gtacggcctg atccacaatt tcctgagggg caaccggcgc aatatcaacg
    47701 tgatcacgcc ctagcattag cgacacaata tgatgcgtgg aggtgttttc gagcatcaca
    47761 tcgcccgcca cctgaccatc gcgcataacg gtggcacagg aggcaatgcg gcggatttct
    47821 tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt tttcaccgca
    47881 ctgatcacca gttcaacttc tgcactggca agcgagctgg tgggttcatc aagaatgacc
    47941 acgcgcggct cgcccttcat cacccgtgca atttccacca gttgcttttg cgccgggctt
    48001 agcgttgaaa caagttgttc aggactaacg tcaacgccca gcgcctgtaa gcaacgttgg
    48061 gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcgaggcca ttgaccaagg
    48121 cagaggtttt ctgccaccgt cagcccttcc accagactta attcctgata aaccgcacga
    48181 acccccagtt cagcggcacg gcgagtcaac gtagcttcgt caccttccag tcgcgtctcg
    48241 ccaatccaga tatcaccgct atccgggcgt tcgctgccgg taagcattcg aatgagagtc
    48301 gatttgcccg cgccgttttt acctaacagc gcacgaactt cgcctttatt gagcgtgaag
    48361 ttaacgttat ccagcgcaac gacgccgggg taacgcttat ttcctgccac cacttttgct
    48421 accgggactg cctctgttgc cgtaaacata gggacctctg cgaatcagcg attagggcag
    48481 accatcaacg tgcgttgcca gccactgttt accatcttcc gttttggtat agagatcgat
    48541 tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca atgtttgcgc
    48601 gaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca gcacctgatt
    48661 gttttccagc tcctgagcaa tttcggttgt catatccgaa ccgaaaacag caatttttcc
    48721 ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgcaccgc ccgactcccc
    48781 cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg aaatcgcttt
    48841 atctaaaaca gtcccttcct gattagcgac aatttgcgcg ccgggaacgc gggattttaa
    48901 tacttcttca aatcctttac gtcgctgcac acaaacttca aaggcttcgc aattgatgac
    48961 ggcaattttc ggctggtaaa ttttattggc aataaaataa tcggcggcag cgttacccag
    49021 ttttttacca aattccagtg gatcgccgac cagatacgcc gagacatatt tatcgacacc
    49081 cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac tggcgcgacg
    49141 aacggtacgg ctactgccat tttcagacac tgccgataaa ataatggcat cgacattacg
    49201 cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcaccctggg cgttagtttc
    49261 aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct gacgcactcc
    49321 agcgtaatat ccctgggtat caaggtatat tgcaccaatg gtcatttctt tatccgcagc
    49381 cctggcaaat agtgcgctac ctaatagcgt agccattaat aatagattac gggttgttct
    49441 cattttttta ggcatagtgc ctcctgtagg gtttttatta acaacggctt attctaatta
    49501 ttttgtgatg agcggcagcg cgtgccgctc aaatattaca gttaataggg tttagtgaac
    49561 gtagttaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc aggccggatt
    49621 aatcgccgtc aattcagcgc gagcagattc atattgcgca acctccccga gtcccgcgga
    49681 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
    49741 cgacaacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
    49801 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcgatttgtt gttcgcccag
    49861 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
    49921 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgcg tcgcttcagt
    49981 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
    50041 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
    50101 gctggcctgc tgcggctgtc tggcatcaag gtgctgccag gcgcgtaata actggttgag
    50161 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tgcgcgtggc
    50221 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcaactctgc
    50281 tgtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
    50341 ctccggtgtg gctccgctta acttatcaag caaatcccgt tcgaaaagca gacgtgcatc
    50401 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttg
    50461 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
    50521 tacgcaacgt tgccataagg taatggcttt gttgtagcta cgcttgttgt agtagaagca
    50581 ggccagtaaa tgacgggcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
    50641 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
    50701 gccgcgttcg gctttcggca ataaactcgc ctgcaggtaa agcggcagcg tgcgctggca
    50761 gtccagtgcg ttcagcatct ctgccgccag ggtgggcatt ccccagttaa tcagttgccc
    50821 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
    50881 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
    50941 acgcagcagt ttcgcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
    51001 cagattatgc aggcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
    51061 aaaatccaga ccagcgtcga agttaccatt acgcgccgcc agtcgtgcca ggccataata
    51121 gccaccggct ttgctgttgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
    51181 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa ctggcctgtc cgcactgcgg
    51241 atttttgttc agcgcatgtg cgcgtttcag agcctgagtg gcatacgcca ccgcttgcgg
    51301 gaaatcggcg cggttatatt ccagcatcgc cagcgccagg ttacagcgat aatccagcgg
    51361 atccagcacc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg
    51421 ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgcgag
    51481 tggtgctttg gcgacgtccg gcaacggcaa ctcttgcggc tgatgttcct gataactcag
    51541 gacaatattg ccgtcggcat cggagagctc aatggtcagc ctttcaggat tgataccgtg
    51601 caacacgccc tggatggcgg tcgcaggcat cagtgccacg gcatcatcaa gtaacgcgtt
    51661 gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga tggcatacag
    51721 cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc gggaggcatt
    51781 ttgcaccatg cccaaagaat gataaggcag gaaatactgc tcaaaacgct tctcttcgta
    51841 agcatcaagc caggtaaaat cgggctggtt atcggcaaaa ataccggtca tcagttcgat
    51901 atacgggccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt cactgtgtcc
    51961 ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca gcaaaccgcc
    52021 atcttcatcg tgacaccacg cgccaacaaa atcgtactgt gatttttcag ccatatatga
    52081 ggttggaacg ggcacatttt tatagcgaga aatgtccact ccggcggagt agtccacttt
    52141 gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac
    52201 cgccgttaca tccggcggga aaacgctctg atgcccttcc ccccctttca ctgccgggtt
    52261 ggcccaccac aagaaatgac gcggcgtggc gttaccgtta tagacgcggc tggcgatttc
    52321 cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac catgcatcgg
    52381 ctccgtttcg cctacccaca ccgtctgtgc accgtcttca tgggcttcga gggtgaaatc
    52441 aacgggcata aaggtggtcg ggcgatgatg ttgcggccag ttaaactcaa tcccaccgga
    52501 aatccacggc cccagcagcc ccaccagcgc aggtttaatg acttcattgt gataaacaaa
    52561 atcgcgctgt ttcactttat cccacgcgcg atgcacccga ccgcccagtt ccggcaggat
    52621 catcactttg atgtagtcgt tttccagcca caccgcctgc caggatttca gggttttctg
    52681 ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc cctgataaac
    52741 gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg tcgggatctc
    52801 aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa caagacatac
    52861 tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg cggaataatc
    52921 acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa tcaacagatt
    52981 cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg cgccaataaa
    53041 tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat tttgcaggaa
    53101 ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa gccagacgcg cgggcatagt
    53161 agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgcctgaa cgtaacgccc
    53221 accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggtga atttgagtat
    53281 ttacagattg atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa atgctggcat
    53341 cgtcatcgca aactgtggcc gggccgcacc atcaatctgg cgctggcaat ccacgggcag
    53401 gttgatccgg tgaccggcgt atcgcaaacc atgccgcaag cgccgtggac aacgccggtt
    53461 gaggtgaagt atctgctgga agagaagctg ggcattcggg tgatggtcga taatgactgc
    53521 gtgatgctgg cgctggcgga gaaatggcaa aataattcgc aggaacggga tttctgcgtg
    53581 atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat ttatcgcggc
    53641 agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg cgtcgtctgc
    53701 gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcatt aaaaaaacag
    53761 gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga aaaactgaca
    53821 accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag ctgggttgat
    53881 cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct caatattaat
    53941 cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa tactattatt
    54001 cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa agcgacgcaa
    54061 attggctttg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta tttgtatgtt
    54121 gaggcgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc cgaaggaagc
    54181 cttttttcaa cagattaacc gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc
    54241 gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt ccagcacgtc
    54301 acacatccag ccagccagtt ctttcgcttc tgcttctttg aagccgcgac gcgtaattgc
    54361 cggagtaccc acgcgaatac cggaggtcac aaacgggctc ttcggatcgt tcggtacgct
    54421 gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag
    54481 gtttttatcg accagatcaa ccaggaacag atggttatca gtaccgccgg aaaccacttt
    54541 gtagccgcgt tccaggaaca cttctaccat cgctttagcg tttttagcga cctgctgctg
    54601 gtaagttttg aactcaggct ccatcgcttc tttcagagca accgctttac cggcgattac
    54661 gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag
    54721 ctcttcgcta ccacctttcg ccaggatcag gccgccgcgc ggacccgcca gggttttgtg
    54781 agtggtggta gtaacaacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac
    54841 caggcccgca acgtgcgcca tatcaacgaa caggtaagca ccgatgctgt cagcgatttc
    54901 acgcattttc gcccagtcca ccacgccgga atatgcagag aaaccaccga taatcatttt
    54961 cggcttgtgt tctttggctt gtttttccag atcggcgtag tcgatatgac cggtagcatc
    55021 gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt
    55081 caggtgaccg ccatgcgcca ggttcatacc cagaacggta tcacctggtt ccagcagcgc
    55141 ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc
    55201 gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca
    55261 accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc
    55321 ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc
    55381 ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat
    55441 gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc
    55501 tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca
    55561 ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa
    55621 taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt
    55681 catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata
    55741 agatgcattt gagatacatc aattaagatg caaaaaaagg aagaccatat gcttgacgct
    55801 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    55861 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    55921 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    55981 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    56041 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    56101 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    56161 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    56221 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    56281 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    56341 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    56401 cagtactctt tgactcgcaa accggatggc aaaggctatc gtattgcggt gaaacgcgaa
    56461 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    56521 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    56581 atctctgccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    56641 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgatgt tcacgccttt
    56701 gccgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    56761 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    56821 ttgagcaaac aggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    56881 gttggcttca tgcagtttgc cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    56941 attcattacg aatgctttgg cccgcataag gtgctgtgat ttgatgttgc cggatggaaa
    57001 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    57061 cgaatgaccc gtgccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    57121 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    57181 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    57241 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    57301 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    57361 atgctattcc ttgaaaaggt cgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    57421 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    57481 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt aatttgcagc
    57541 agcttacgca aatagttgag ttcaaactga ttacgtgcct caacaaaggt tggcagcgcc
    57601 gtgttttcac cctccagcgc ctgctctact aatgcatcgc taataaccgg agatgaggtc
    57661 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctagcg
    57721 gtcatcaggc gtttcatcgc atcagtggag aacgcgcgga caaacggttt atgtcgctct
    57781 gccgcctggc gcaacaggtg atttgccaga agcggaatgt cttctgtgcg ctctgccaga
    57841 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    57901 cgcgccatcg cttttggcag atcacggtga gtggcagaaa taatccgcac gtcgatatca
    57961 atatcgcggt tgctgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    58021 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    58081 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    58141 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    58201 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    58261 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    58321 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    58381 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcgtcttt gtcgacaggc
    58441 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    58501 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    58561 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    58621 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    58681 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    58741 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    58801 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    58861 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    58921 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    58981 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    59041 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    59101 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    59161 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    59221 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    59281 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    59341 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    59401 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    59461 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    59521 acagcgcgga gaaaccggag cgtacatatc gtacgtgaag atttcgagca ctgcccgggg
    59581 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    59641 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    59701 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    59761 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    59821 tcttgcggaa ttggcgtgcc ggtattgatg acatcaatat aaacccgcgc accatgtgaa
    59881 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    59941 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    60001 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    60061 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    60121 taatcaagca gttgttcgat cagtttttgc aaattgcggc tgctgctatc aagaatgctc
    60181 accacctctt tttgctctgg cgtaagcggc ccgacaacct ggtcagccag taattcagtg
    60241 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    60301 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    60361 gagcgtaact cgctcggtcc actgaacgaa acgctattgc ccagagaacg cccttccccc
    60421 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    60481 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    60541 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    60601 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    60661 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    60721 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    60781 cgctggcttt gataaacctt cgcaagcgta ggatcgtcca gcacgcaata ctgacggtag
    60841 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    60901 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    60961 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    61021 aatgagcggg gaaaaacagg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    61081 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    61141 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    61201 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    61261 gaaggggctg acataagtcg gtgaatgagc cactgcttac tatatgcagt aactgtgcca
    61321 ataaagaaaa tagtttggta acgtactgat tatacgtcgc tttgaggggt ttatggcttc
    61381 tccgttattt gaatgtaaat cagccactgt gtcgc
//
LOCUS       DAZFWP020000007        61170 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFWP020000007 DAZFWP020000000
VERSION     DAZFWP020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 61170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.007"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..254)
                     /locus_tag="ACHK0Z_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             complement(<1..254)
                     /locus_tag="ACHK0Z_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4500474.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQ"
     gene            467..1258
                     /locus_tag="ACHK0Z_RS01970"
                     /old_locus_tag="pgaptmp_000394"
     CDS             467..1258
                     /locus_tag="ACHK0Z_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HGU4500475.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1268..2110
                     /locus_tag="ACHK0Z_RS01975"
                     /old_locus_tag="pgaptmp_000395"
     CDS             1268..2110
                     /locus_tag="ACHK0Z_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4500476.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2120..2896
                     /locus_tag="ACHK0Z_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             2120..2896
                     /locus_tag="ACHK0Z_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HGU4500477.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            2906..4447
                     /locus_tag="ACHK0Z_RS01985"
                     /old_locus_tag="pgaptmp_000397"
     CDS             2906..4447
                     /locus_tag="ACHK0Z_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4500478.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4444..6525
                     /locus_tag="ACHK0Z_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             4444..6525
                     /locus_tag="ACHK0Z_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HGU4500479.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6546..7022
                     /locus_tag="ACHK0Z_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             6546..7022
                     /locus_tag="ACHK0Z_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4500480.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            7065..8366
                     /locus_tag="ACHK0Z_RS02000"
                     /old_locus_tag="pgaptmp_000400"
     CDS             7065..8366
                     /locus_tag="ACHK0Z_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGU4500481.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            8419..9390
                     /locus_tag="ACHK0Z_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             8419..9390
                     /locus_tag="ACHK0Z_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGU4500482.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENFYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(9502..9951)
                     /gene="rplI"
                     /locus_tag="ACHK0Z_RS02010"
                     /old_locus_tag="pgaptmp_000402"
     CDS             complement(9502..9951)
                     /gene="rplI"
                     /locus_tag="ACHK0Z_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU4500483.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(9993..10220)
                     /gene="rpsR"
                     /locus_tag="ACHK0Z_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             complement(9993..10220)
                     /gene="rpsR"
                     /locus_tag="ACHK0Z_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU4500484.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(10225..10539)
                     /gene="priB"
                     /locus_tag="ACHK0Z_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             complement(10225..10539)
                     /gene="priB"
                     /locus_tag="ACHK0Z_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU4500485.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(10546..10941)
                     /gene="rpsF"
                     /locus_tag="ACHK0Z_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             complement(10546..10941)
                     /gene="rpsF"
                     /locus_tag="ACHK0Z_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU4500486.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            11268..11543
                     /gene="yjfY"
                     /locus_tag="ACHK0Z_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             11268..11543
                     /gene="yjfY"
                     /locus_tag="ACHK0Z_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU4500487.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDMQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(11672..12358)
                     /gene="ulaF"
                     /locus_tag="ACHK0Z_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             complement(11672..12358)
                     /gene="ulaF"
                     /locus_tag="ACHK0Z_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU4500488.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12358..13212)
                     /gene="ulaE"
                     /locus_tag="ACHK0Z_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             complement(12358..13212)
                     /gene="ulaE"
                     /locus_tag="ACHK0Z_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU4500489.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(13222..13872)
                     /gene="ulaD"
                     /locus_tag="ACHK0Z_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             complement(13222..13872)
                     /gene="ulaD"
                     /locus_tag="ACHK0Z_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4500490.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(13886..14350)
                     /gene="ulaC"
                     /locus_tag="ACHK0Z_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             complement(13886..14350)
                     /gene="ulaC"
                     /locus_tag="ACHK0Z_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU4500491.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(14360..14665)
                     /gene="ulaB"
                     /locus_tag="ACHK0Z_RS02055"
                     /old_locus_tag="pgaptmp_000411"
     CDS             complement(14360..14665)
                     /gene="ulaB"
                     /locus_tag="ACHK0Z_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU4500492.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSKIDHTVNSCAVGEY
                     KSELSNADIIIASTHIAGEISVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(14681..16078)
                     /gene="ulaA"
                     /locus_tag="ACHK0Z_RS02060"
                     /old_locus_tag="pgaptmp_000412"
     CDS             complement(14681..16078)
                     /gene="ulaA"
                     /locus_tag="ACHK0Z_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4500493.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYN
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            16433..17497
                     /gene="ulaG"
                     /locus_tag="ACHK0Z_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             16433..17497
                     /gene="ulaG"
                     /locus_tag="ACHK0Z_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU4500494.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVASGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            17605..18360
                     /gene="ulaR"
                     /locus_tag="ACHK0Z_RS02070"
                     /old_locus_tag="pgaptmp_000414"
     CDS             17605..18360
                     /gene="ulaR"
                     /locus_tag="ACHK0Z_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU4500495.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(18357..19106)
                     /gene="yjfP"
                     /locus_tag="ACHK0Z_RS02075"
                     /old_locus_tag="pgaptmp_000415"
     CDS             complement(18357..19106)
                     /gene="yjfP"
                     /locus_tag="ACHK0Z_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4500496.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            19288..19617
                     /gene="bsmA"
                     /locus_tag="ACHK0Z_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             19288..19617
                     /gene="bsmA"
                     /locus_tag="ACHK0Z_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU4500497.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            19766..20041
                     /gene="yjfN"
                     /locus_tag="ACHK0Z_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             19766..20041
                     /gene="yjfN"
                     /locus_tag="ACHK0Z_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU4500498.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(20158..21783)
                     /gene="aidB"
                     /locus_tag="ACHK0Z_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             complement(20158..21783)
                     /gene="aidB"
                     /locus_tag="ACHK0Z_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU4500499.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(21867..23030)
                     /gene="yjfC"
                     /locus_tag="ACHK0Z_RS02095"
                     /old_locus_tag="pgaptmp_000419"
     CDS             complement(21867..23030)
                     /gene="yjfC"
                     /locus_tag="ACHK0Z_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4500500.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(23033..23671)
                     /gene="yjfM"
                     /locus_tag="ACHK0Z_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             complement(23033..23671)
                     /gene="yjfM"
                     /locus_tag="ACHK0Z_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU4500501.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(23681..24079)
                     /gene="yjfL"
                     /locus_tag="ACHK0Z_RS02105"
                     /old_locus_tag="pgaptmp_000421"
     CDS             complement(23681..24079)
                     /gene="yjfL"
                     /locus_tag="ACHK0Z_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU4500502.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(24097..24756)
                     /gene="yjfK"
                     /locus_tag="ACHK0Z_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             complement(24097..24756)
                     /gene="yjfK"
                     /locus_tag="ACHK0Z_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU4500503.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(24807..25505)
                     /gene="yjfJ"
                     /locus_tag="ACHK0Z_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             complement(24807..25505)
                     /gene="yjfJ"
                     /locus_tag="ACHK0Z_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU4500504.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(25524..25925)
                     /gene="yjfI"
                     /locus_tag="ACHK0Z_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     CDS             complement(25524..25925)
                     /gene="yjfI"
                     /locus_tag="ACHK0Z_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU4500505.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSQVDNALDLAEITEEYSH"
     gene            complement(26052..26783)
                     /gene="rlmB"
                     /locus_tag="ACHK0Z_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             complement(26052..26783)
                     /gene="rlmB"
                     /locus_tag="ACHK0Z_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU4500506.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(26963..29404)
                     /gene="rnr"
                     /locus_tag="ACHK0Z_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             complement(26963..29404)
                     /gene="rnr"
                     /locus_tag="ACHK0Z_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU4500507.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLSKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(29443..29868)
                     /gene="nsrR"
                     /locus_tag="ACHK0Z_RS02135"
                     /old_locus_tag="pgaptmp_000427"
     CDS             complement(29443..29868)
                     /gene="nsrR"
                     /locus_tag="ACHK0Z_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU4500508.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(30073..31371)
                     /gene="purA"
                     /locus_tag="ACHK0Z_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             complement(30073..31371)
                     /gene="purA"
                     /locus_tag="ACHK0Z_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU4500509.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(31475..31672)
                     /gene="yjeT"
                     /locus_tag="ACHK0Z_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             complement(31475..31672)
                     /gene="yjeT"
                     /locus_tag="ACHK0Z_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU4500510.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(31754..32758)
                     /gene="hflC"
                     /locus_tag="ACHK0Z_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             complement(31754..32758)
                     /gene="hflC"
                     /locus_tag="ACHK0Z_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU4500511.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(32761..34020)
                     /gene="hflK"
                     /locus_tag="ACHK0Z_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             complement(32761..34020)
                     /gene="hflK"
                     /locus_tag="ACHK0Z_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU4500512.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(34106..35386)
                     /gene="hflX"
                     /locus_tag="ACHK0Z_RS02160"
                     /old_locus_tag="pgaptmp_000432"
     CDS             complement(34106..35386)
                     /gene="hflX"
                     /locus_tag="ACHK0Z_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU4500513.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(35463..35771)
                     /gene="hfq"
                     /locus_tag="ACHK0Z_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             complement(35463..35771)
                     /gene="hfq"
                     /locus_tag="ACHK0Z_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU4500514.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(35857..36807)
                     /gene="miaA"
                     /locus_tag="ACHK0Z_RS02170"
                     /old_locus_tag="pgaptmp_000434"
     CDS             complement(35857..36807)
                     /gene="miaA"
                     /locus_tag="ACHK0Z_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU4500515.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(36800..38647)
                     /gene="mutL"
                     /locus_tag="ACHK0Z_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             complement(36800..38647)
                     /gene="mutL"
                     /locus_tag="ACHK0Z_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU4500516.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(38657..39994)
                     /gene="amiB"
                     /locus_tag="ACHK0Z_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             complement(38657..39994)
                     /gene="amiB"
                     /locus_tag="ACHK0Z_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU4500517.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(40013..40474)
                     /gene="tsaE"
                     /locus_tag="ACHK0Z_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             complement(40013..40474)
                     /gene="tsaE"
                     /locus_tag="ACHK0Z_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU4500518.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(40446..41993)
                     /gene="nnr"
                     /locus_tag="ACHK0Z_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             complement(40446..41993)
                     /gene="nnr"
                     /locus_tag="ACHK0Z_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU4500519.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            41992..43131
                     /gene="queG"
                     /locus_tag="ACHK0Z_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             41992..43131
                     /gene="queG"
                     /locus_tag="ACHK0Z_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU4500520.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(43114..43167)
                     /locus_tag="ACHK0Z_RS02200"
                     /old_locus_tag="pgaptmp_000440"
     CDS             complement(43114..43167)
                     /locus_tag="ACHK0Z_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4500521.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(43401..43476)
                     /locus_tag="ACHK0Z_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     tRNA            complement(43401..43476)
                     /locus_tag="ACHK0Z_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43441..43443),aa:Gly,seq:gcc)
     gene            complement(43512..43587)
                     /locus_tag="ACHK0Z_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     tRNA            complement(43512..43587)
                     /locus_tag="ACHK0Z_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43552..43554),aa:Gly,seq:gcc)
     gene            complement(43624..43699)
                     /locus_tag="ACHK0Z_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     tRNA            complement(43624..43699)
                     /locus_tag="ACHK0Z_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43664..43666),aa:Gly,seq:gcc)
     gene            complement(43910..44455)
                     /gene="orn"
                     /locus_tag="ACHK0Z_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             complement(43910..44455)
                     /gene="orn"
                     /locus_tag="ACHK0Z_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4500522.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            44550..45602
                     /gene="rsgA"
                     /locus_tag="ACHK0Z_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     CDS             44550..45602
                     /gene="rsgA"
                     /locus_tag="ACHK0Z_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4500523.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            45699..46667
                     /gene="asd"
                     /locus_tag="ACHK0Z_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     CDS             45699..46667
                     /gene="asd"
                     /locus_tag="ACHK0Z_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4500524.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEN
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            46689..50012
                     /gene="mscM"
                     /locus_tag="ACHK0Z_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     CDS             46689..50012
                     /gene="mscM"
                     /locus_tag="ACHK0Z_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4500525.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPIRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(50163..51665)
                     /gene="yjeM"
                     /locus_tag="ACHK0Z_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     CDS             complement(50163..51665)
                     /gene="yjeM"
                     /locus_tag="ACHK0Z_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4500526.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(51884..52861)
                     /gene="epmA"
                     /locus_tag="ACHK0Z_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             complement(51884..52861)
                     /gene="epmA"
                     /locus_tag="ACHK0Z_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4500527.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            53186..54994
                     /gene="frdA"
                     /locus_tag="ACHK0Z_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             53186..54994
                     /gene="frdA"
                     /locus_tag="ACHK0Z_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4500528.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            54987..55721
                     /gene="frdB"
                     /locus_tag="ACHK0Z_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             54987..55721
                     /gene="frdB"
                     /locus_tag="ACHK0Z_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4500529.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTNFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            55732..56127
                     /gene="frdC"
                     /locus_tag="ACHK0Z_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             55732..56127
                     /gene="frdC"
                     /locus_tag="ACHK0Z_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4500530.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            56138..56497
                     /gene="frdD"
                     /locus_tag="ACHK0Z_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             56138..56497
                     /gene="frdD"
                     /locus_tag="ACHK0Z_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4500531.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            56560..57693
                     /gene="ampC"
                     /locus_tag="ACHK0Z_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             56560..57693
                     /gene="ampC"
                     /locus_tag="ACHK0Z_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4500532.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            57782..58315
                     /gene="blc"
                     /locus_tag="ACHK0Z_RS02275"
                     /old_locus_tag="pgaptmp_000455"
     CDS             57782..58315
                     /gene="blc"
                     /locus_tag="ACHK0Z_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4500533.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(58312..58629)
                     /gene="sugE"
                     /locus_tag="ACHK0Z_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             complement(58312..58629)
                     /gene="sugE"
                     /locus_tag="ACHK0Z_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4500534.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(58811..58957)
                     /gene="ecnB"
                     /locus_tag="ACHK0Z_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             complement(58811..58957)
                     /gene="ecnB"
                     /locus_tag="ACHK0Z_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4500535.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(59068..59193)
                     /gene="ecnA"
                     /locus_tag="ACHK0Z_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             complement(59068..59193)
                     /gene="ecnA"
                     /locus_tag="ACHK0Z_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4500536.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(59245..59811)
                     /gene="efp"
                     /locus_tag="ACHK0Z_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             complement(59245..59811)
                     /gene="efp"
                     /locus_tag="ACHK0Z_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4500537.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            59853..60881
                     /gene="epmB"
                     /locus_tag="ACHK0Z_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     CDS             59853..60881
                     /gene="epmB"
                     /locus_tag="ACHK0Z_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4500538.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT        14844 a        16322 c        15368 g        14636 t
ORIGIN      
        1 ttttgtggcg gaagatctga gaaatcaagg gccttttctt tttgtcgaat atcgttccag
       61 gtatattcca gtacttcaat aaataaattt tctttattac caaagtgatg ataaaccgtc
      121 tgtttactac accccgcagc cttaacaata ttatcgaccc tggccccttc ataaccatat
      181 tcggcaaatt cattaaccgc gcataacagc agcttttcct tcaggctctg tacgttgctg
      241 gtatcaagat acataacgct tccccacgac ctcttcaaga taactatctt aacccgatta
      301 attagaagct ccagtaaaaa ggataaaaac ttgtactttc atcaagttat caaatgtcct
      361 gtgatcacta tcgcatatca accaaccaga tggttgatct ttcttgcggc acgtcgcaag
      421 ctaattgtca cttagaaaac aatattcaaa aaaagagagc tattgtatga aatccactcg
      481 tcctgttgcc gtgattaccg gagccgctcg tggtattgga aagggctgtg cgctggagct
      541 tgctcgtggc ggatttaacc tgctgattaa cgatcttccc gatgccgaca gcgtagaaaa
      601 actgcatatc acacaacaag aatgtatagc tgaaggcgtg gaagtgattt gcttccctgc
      661 cgatgtcggc gatctctcct tgcatgaaga gatgctcgac gcggcgcaaa accagtgggg
      721 acgtctggac tgcctgctca acaacgcagg catttcagtg aaaaaacgcg gtgaccttct
      781 cgacctcgaa ccagacagct tcgaccaaaa tattgccatt aacacccgcg cgccgttctt
      841 cctcgctcag gcattcagta aacgtctgct cgcacagccg aaaccggaag cagaactgcc
      901 gcaccgctcg attattttcg tcagttccat caacgccatc atgctggcga tgaatcgcgg
      961 cgaatatacc attgccaaaa ccgccgtttc tgccgctgct cgtctgtttg ctgctcgcct
     1021 ttgtaatgaa caaattggcg tttatgaagt gcgccccggt ctcatcaaaa ccgatatgac
     1081 tattccggca acggcttatt acgacgaact gattgccaaa ggtctggtgc cgtggggacg
     1141 ttggggttat ccggctgata ttgcttccac tgtccgcgcg atggcggaag gtaaactgat
     1201 ttacacctgt ggtcaggcgg tcgccatcga cggcggctta agtatgccgc gcttctgagg
     1261 ctgaatcatg agagatctgt tacaacgccc ggatttattc agtatcaata ccgcaacact
     1321 gggctataaa acgccgctac ccgccattat tgatgcctgc gccgcgcgtg gtattggcgc
     1381 gattgctccc tggcgcaggg agttgcaagg tgaagatctc cagcaaatcg cccaccagct
     1441 tgccgccagt aacatgagcg tttccggttt gtgccgtagc acttactaca ccgcgccgac
     1501 actggcggaa cgcaaactgg cgattgatga taaccgccgg gcgctggacg acgcagcggt
     1561 gcttaacgcc gcctgttata tgcaggtggt tggcggtctg ccaacgggca caaaagatct
     1621 gtacgaagcg cgggaacaag taaaacaagg gattcgccag ttgctgccac attcgaaaga
     1681 tgtcggcgtg ccaattgcgc tggagccact gcacccgatg acagctgccg atcgctcttg
     1741 cctgtgcacg ttacgccagg cgctggactg gtgtgatgaa ctcgatcccg atggtgagtt
     1801 tggcctcggc gtggcggtgg atgtttatca cgtctggtgg gatccggatc tcgccagtca
     1861 gatcctgcgt gccggaaaac gcatcctcgc gtttcatgtt tccgactggt tagtgccgac
     1921 cactgatctg gtcaatgacc gtggtatgcc gggagatggc gtcatcaata ttccgtcaat
     1981 tcgtcggctg gttgaaaacg ccggatttaa tggtgctatt gaactggaaa tattctcgcc
     2041 ttactggtgg cagaaagata ttaacagcac gctggatatt agcgtcgatc gcatcgcgca
     2101 ttattgttaa ggaatgatca tgagtgacca acccgttctg ttcagcagag tggcggcttc
     2161 ctgccgcctg acattgaacc gtgaagataa atgtcatgct attaacgaag agatgatcga
     2221 atctctcgac cattatttaa atgaaattga aaacgacacc acattacgat tagtcgaatt
     2281 aacggcaacc ggcgataaat tcttttgtgc gggcggcgat attaaatcgt ggtcggccta
     2341 ttcgccatta gatatgggca gaaaatggat taaacgcggt aatgacgttt ttaatcgcct
     2401 gcgaaactta ccccaattaa ccgttgcgaa tctcaacggt cataccatcg gcggtggtat
     2461 tgaactggcc ttgtgctgcg atattcgtat tgcccgcccc ggcgcgaaat tctctaaccc
     2521 ggaagtgatg ctcggcatgg tgcctggctg gatgggcatt gaacgtgtgc ttaatcaggt
     2581 tggtccggtg gtcggtcgcc agatgctgat gctgggtaaa cgcctgacgg cgcaggaagc
     2641 ccaggcggcg aatttaattg atgaagtggt agaaaaagaa caggtggaaa gctggatggc
     2701 gaaccagtta gcgcagctgg aaaaatgcgg tccggtggcg cttgcacata ttaagcaact
     2761 gattctggca ctggaaaata aacatgccga ttacccgcac cagctactgg ccggattaat
     2821 gtccgccact caggattgcc agcaggcgac gcgcgccttt gccgaaaaaa gtagcgtaag
     2881 cttccataat caataaggaa attgtatgcg taagataaca accgccgagg cactggcggc
     2941 gcaaattcag gacggtgcga ctattgctat tagcggtaac ggcggcggta tggtggaagc
     3001 ggaccatatt ctggctgcta ttgaagcgcg tttcctgcaa accggacacc ctcgcgacct
     3061 gacgctaatc cactcgctgg gtattggcga tcgcgactgc aaaggcacta atcgttttgc
     3121 gcacgcagag atgctcaaac gcattattgc cgggcacttt acctggtcgc ccaaaatgca
     3181 ggcgctggta aaaaataaca cgattgaagc ctactgcttt cctggtggcg tgattcaggc
     3241 actgctacgg gagatcggcg ccggacgtcc ggggcttttt acccacgttg ggctgggatc
     3301 gtttgttgat ccacgcaatg gcggcggtaa gtcgaatgaa tgcactaccg acgacctggt
     3361 agaactgatt gaaatcgatg gtgaaaccaa acttcgttat cgccctttca aagtggatta
     3421 cgcgattttg cgtggcactt atgccgatcc tcgaggcaac gtcagccttg aagaagaagc
     3481 gattgatatg gatagctatt ccatggcgct ggcagcacac aacagcggcg gcaaagtgtt
     3541 cgtacaggta cgcgatgtgc tggaagctgg cgcgattgaa ccacgccggg tcaaattacc
     3601 gggcattctg gttgatggca tcgttgaaca ccgcgaacaa ccgcaaacct atcttggcgg
     3661 ttacgacctg accattagcg gtcaacatcg ccgtctgagt tctaacgacg ctattgaact
     3721 ggttagtcat ccggtgcgcc gcctgattgc ccgtcgggca gcacgcgaac tggtggctgg
     3781 cgcttcaacc aactttggct ttggtattcc tggcggtatt ccaggcgtag cgctgcgcga
     3841 aggcgttcct taccaaagtt tatggctgag cgtcgagcaa ggcgtacata acggcatgat
     3901 gcttgatgat gcgtttttcg gctgcgcccg caacgccgat gccattattc catcactcga
     3961 tcaatttgag ttctacagtg gcggagggat cgatatcacc ttcctcggca tgggagagat
     4021 ggatcagtac ggtaacgtca acgtctccca tctcaatggc aatctcattg gtcccggcgg
     4081 ttttctcgaa attgcgcaaa acgcccgtaa agtggtgttc tgcggcacgt tcgacgccaa
     4141 aggtagcaag attgatgtaa cgccagatgg cttgcatatc acccagtcag gtcaaatccc
     4201 taaactggtt acccaggtgg aaaaaatcac ttttagcgcc gcctacgcac agcaaagtgg
     4261 tcaggaagtg ttgtatatca ctgaacgcgc agtattccag ttaacggcag aaggcgttga
     4321 attaattgaa atcgcgccgg gtgtggagat tgagcgcgac attctgccgt atatggcttt
     4381 ccgtccaatt atcaagcacc cacgcctgat ggaaagtagc ctgtttacgc cgatggagga
     4441 tgcatgagtc agcttgatga caccatcctt gatgcgctga cgcacgttac tttcccaaag
     4501 ggttttgcac aggcagagcc cgcatgggtt gtcacggtag acggtgttga ttacccactc
     4561 tggcaaacag atgccctggt agtcggcagt ggtgcagcag gattacgtgc ggcagttgaa
     4621 cttaaacgcc gccagcaaaa tgtgctgatc gctaccgccg ggttatatat ggggacgtcg
     4681 gcatgttctg gttccgataa acagacacta tttaccgccg ctaccgcggg caacggcgac
     4741 aacttcacca aactggcaga ggcactggcg agcggtgggg cgatggatca cgacaccgct
     4801 tatgtcgaag cggtaggttc tctgcacact ctaggcgggt tgcaatatct tggtctggaa
     4861 ttaccggaag atcgctatgg cgcgattctt cgttatcaaa ccgaccatga cgaagccggg
     4921 cgtgcaacct cgtgtgggcc gcgtacctca aggttaatgg tgaaagtgct gttggaagaa
     4981 gtacagcgcc tcgccattcc agtgttgacc agtgcaacag tgattaaact gctgcatcag
     5041 cgtgacgaaa acggcgaaga ccgcgtggca ggggcaatcc tcgcgaccgg tcatcgcgcc
     5101 cataacccat gggggctggc aattgtgact gcgcccaatg tggtactggc aacgggcgga
     5161 ccgggcgagc tttatcgcga cagtgtttat ccacataaat gcttcggctc gctggggctg
     5221 gcgctggagg aaggtctgac gctaaccaat ctgaccgaaa gccagtttgg tataggcacg
     5281 ccgcgtagca cgtttccgtg gaatttatcc ggcacctatg tgcaggtgat cccgtatatc
     5341 tattccgtgg atgctgaggg taacgagtat aacttcctcg cggattacta tcgcaccacc
     5401 caggagctgg cttcaaacat tttccgtaaa ggctatcagt ggccgttcca cgccactcgg
     5461 gtgatggatt ttggctccag cttgttagat atggcagtag cgcaagagca gcaatcaggg
     5521 cgtcaggtat ttatggattt caatcgcaat cctgaagcgg tgccgggtga cctgccgttc
     5581 tcattagatc gactggacga cgacgttcgc gcgtatctgg aaaataacga cgctctggca
     5641 ccatcgccca tcgaacggct gcaacgaatg aatccgctgt ctatctcgct gtataagatg
     5701 cacggttacg atctcaccac acagcctttg cagtttgcca tgaataatca gcatatgaat
     5761 ggcggcattg aagtggatat ctggggacaa acctccctgc ccggctgttt cgccgtgggg
     5821 gaagttgctg gcacacacgg cgtcactcgc cctggtggtg cagcattgaa tgccgggcag
     5881 gtttttgctg ttcgtctggc acgttttatt ggttgcacgc aaaagcggaa tattgatgga
     5941 gatatagcac agctggtagc tcctacactg gcttctataa gagagatcat tactcaggcg
     6001 cacgataacg ggaccgggat gccgttgtcg gttgtgagag aaaaaattca ggcacgaatg
     6061 tctgaccatg cgggatttat ttgccatgcc gataaagtcc gacgcgccac tcgcgatgcc
     6121 ctgctattga gcgaatttgt ccaacggcat ggattggcta tcaaacatgt gggtgaagtt
     6181 gccgagctgt ttatgtggcg gcatatggca ctgacttctg ccgccgtatt aacgcaatta
     6241 acgcattata ttgacgcagg tggtggtagt cggggagcac gtattattct cgacagggat
     6301 ggaaattcta ttccgcaaac acgaaatggt ttttgtgatg cctggcgctt tcgttcagag
     6361 cgtactgagg ataaaaaaga taaactactt attcattatt gcaacggtat ctttcacgtc
     6421 agagaaacac ctgttcgtga gtttcctatt atccgaggta tttggtttga aaaaaactgg
     6481 cctggttttc ttaatgggac aatttaccag ccacaggatg aatgatattg caaaggttaa
     6541 tcactatgcg aaatataatt agatctatta atgctctact tgcagcattg aacattacca
     6601 ttttggcgat catcgtggca tgtgtgacgt ggcaggtggc tgcacgattt atatttacta
     6661 gtcccagtat ttttacagat gaactcagcc gtttgctctt tatttgtctt ggactttttg
     6721 gtggggcata caccgcaggg caaaaccggc atctggcgat tgacctgcta cctatgatgc
     6781 tgaaaggaaa agcccgacgt catttatttt tgtgtattca gattatcgtt attatcttcg
     6841 caaccatcat tatggtatat ggtggtgggc tattaacgat ggatacattc gattcaggtc
     6901 agacctcgcc cgctttaggc tggcaaatgg gatatatcta tatgagcatc ccgattagtg
     6961 gtgtacttat tattatatac accatagata tggttttaac cgaacttaaa caaccactct
     7021 aaagataaca aacaacaacc aaccatagta ggaatggaga aattatggat tactggttac
     7081 cgattattgt gttatttggt gccttctttt ttatgttggc attaggtgta cctattgttt
     7141 atgccattgg tttatcgaca ctggcatcaa tttccacaca actcgatttc aactcagcat
     7201 tgagtgtggt ttcccaaaag ttagcctccg ggttagatag ttttacgttg ttggcaatcc
     7261 cattttttat cttgtctggc aacattatga accatggagg aatagcgcga cgattaatca
     7321 actttgcccg tattctcggc ggtcgtctgc caggctcact tgcccactgt aatattctcg
     7381 ccaatatgct gttcggggca atttctggtt cagccgtggc ttcggcggct gcgatgggcg
     7441 gcgtcatgca tccgcaacag gttaaagagg gttacgaccc ggcattcagt actgcggtta
     7501 acgttgcctc tgccccgacg ggtttgctta ttccaccaag taatacgtta atcgtctatt
     7561 cactggtcag tggtggtact tctattgccg cactgttcct ggcgggttat gttccgggaa
     7621 ttttgctggg tctggcactg atggtaatag ccggtattat cgcggtacgt cgcggttatc
     7681 caaagccaga acgcccgact ttacgccagg ctggcgttgc gatatggatg gctatcccca
     7741 gtattttcct gattatttta attatggggg gtgtattaag tggtattttt accccgacag
     7801 aggcctccgc aattgcagta atttataccc ttttccttgc actggttctt tatcgtgaaa
     7861 tctcagttaa agatttacct aaaatatttc tggaatccgt tattaccact gcaattgtct
     7921 tgttattaat tggttcttcg atgggtatgt catgggcaat gtcaaacgct gacgttccct
     7981 tcctgattct cgatttgctt aacactattt ctgacaaccc aataataatc ctgcttatta
     8041 tcaatattat cctgttgatt atcggcactt ttatggatat gacaccggcg gtactgattt
     8101 ttacgccgat atttttaccg gttgtcaccg aactgggcat ggacccgatt cactttggta
     8161 ttgtcatggt actgaacatg tgtatcggta tttgtactcc gcctgtaggc agtgtgttat
     8221 ttgtcggttg tagtgtatct aaattaccga ttaataaaat tatcaaacct atgctgccat
     8281 tctatgccgt gatggtattg gtgctggcaa tggtgaccta tattccgcaa atcagtatgg
     8341 cattgccaag agcactgggc tactgatgcg cattcactgg cggcacctgc cgccatattt
     8401 attagaaaag gaacatctat gaaaataaaa gtatccgctg gcattatcgg tgctgttctt
     8461 atgttatccg caagccagtc ctgggcagtg acattaaaac tgagtcataa tcaggataag
     8521 tctcatcctg ttcataaagc gatggagttc tttgcgaaaa agagcaaaga gtactctaac
     8581 ggtgatatta ctattcgtat ttatccaaat ggaacattgg gtactcaacg agaaacaatg
     8641 gagctgattc gttctggcgc tattcccctg gtaaaaacca atgcggcaga aatggaagca
     8701 tttgaaaatt tctataaatt atttagcctg ccttatttgt tccgcgatcg tgatcattat
     8761 tatcaggtca tgcagggcga tatcgggaga aaaatcctcg actcaacgaa aagcaaaggt
     8821 tatttcgggc tgacttttta tgatggaggc gcccgcagtt tctatggcaa taaaccagta
     8881 ctgaaaccag acgatctgaa aggcatgaaa gtccgtgtcc agccaagccc tggcgcagtt
     8941 gaaatgatca aagtcatggg cggtaacccg acgccactgg attacggcga gttgtataca
     9001 gccttacagc agggtgtggt cgatatggca gaaaacagcg tgatggcgct gaccaccatg
     9061 cgtcacggtg aagtggcaaa atccttcagc cttgacgaac acactatggt tcccgatgtg
     9121 gttctgatga gcaatgctgc gtttgataaa cttagcccgg aaaatcaggc agttatatta
     9181 aaagcagcta aagaatcaat gagctatatg aaagacttgt ggagcgagga agagaaacaa
     9241 gaatttgcga aactggataa aatgggcgtg aaagtctacc aggtagataa agctccgttt
     9301 atcgagaaag tacagccaat gtacgcaaac ttcgctaagg acaacccagc ccttgcccca
     9361 atgctggctg atattcaggc agctaagtaa ttatcttcgt agggctgctt attgtggccc
     9421 ttatcgatat ctgcaagtgc tcgctacaaa agcaaaacgc cgaccaatgg tcggcgtttt
     9481 tacgtctcgt tgaataacga attattcagc tactacgttt acgatcactt tcgcgaatac
     9541 ttcgctgtga acctggaagc tcacttcgtg ttcgccagtg gtacgcagaa cgccgttcgg
     9601 cagacgaact tcgctcttag ccacttcaac gccagctgca gttacagcgt cagcgatgtc
     9661 gcgagtaccg atggaaccga acagtttacc ttcgtcgcca gctttagacg cgatggtaac
     9721 agtttccagt gcattgattt tctcagcgcg agcattagca gctgccagaa cttcagccag
     9781 tttagcttcc agttcagcgc gacgtgcttc gaagaattca atgtttttct tggtagctgg
     9841 aacagcttta ccctgcggta ccaggaagtt acgagcatag cccgctttaa cgtttacctg
     9901 atcacccagg ctacccaggt ttgctacttt atcaagcaga ataacttgca ttaccttatc
     9961 ctctcaaagt cgtattaatg gaccgtgacc gattactgat ggcgatcagt gtacggcagc
    10021 agggacaggt agcgagcgcg tttgatagcg cgagccagct gacgctggta ttttgcacgg
    10081 gtaccggtga tacggcttgg gacaatctta ccgctttcgg tgatgtagtt tttcagcgta
    10141 gcgatatctt tatagtcgat ctcttgaacg ccttccgcgg tgaaacggca gaacttgcga
    10201 cgacggaaat aacgtgccat atggctagtc tccagaatct atcaattcaa tctgctcggc
    10261 atgcaaaacc attttgctca gtccgttctt tgccttgtgg catgaaatga acccctgaac
    10321 ggttatgcga ctgccgaccg ttatactgtg agtaatggcc tggttttcgt gtccgctaac
    10381 aataacgggc atttgacacc atgcctgccg gtgaaagccg gcttcctcct gcacagaacg
    10441 atgctcaagc acgaactggc agtgaggaat tcctgatgga ctgacctttc gaaggggagt
    10501 cctgcacacg gtgccggaca acaccagacg gttggtcatc agaaattact cttcagaatc
    10561 cccagcttca gcatcatcag cggtttcgtt tgcgaaatca tcgcgacgct cacggcgctc
    10621 gtctttcgct ttaaccatcg gagatgcttc ggtaacagcg tgcttggtac gcataaccat
    10681 gctgcggata acggcatcgt tgaagcggaa ggtagtttcc agctcatcga tcacttcctg
    10741 cggagcttca acgttcatca gaacgtagtg tgctttgtgc agtttgttga tcgggtaagc
    10801 cagctgacgg cggccccagt cttccagacg gtggatcttg ccttctgcac cagtgatggc
    10861 agcagtgtag cgctcgatca tgcccggaac ctgttcgctc tggtcaggat ggaccataaa
    10921 aacgatttcg taatgacgca tcgaattgct ccttacggat tattcagcct cctgtctggg
    10981 tcagccgaat cccggggagg caaggaacgt gttaaaggtc ggctgaaaaa tgacgcgtta
    11041 tagtacttgt atgccccgct aaactcaagg gcagagccga aataaatctc ccctttcgca
    11101 attaacctct aaaccgctga tttggagttc atcacacttc atcatggtgt ttttttgaac
    11161 aactgcatca gaaatggtca ccatcaactt ctttatcatt gattaaaatt aaatcagaag
    11221 atcatcacag aggagagcac acaaagtcag ccgtcgagga ggtatctatg ttcagtcgag
    11281 ttttagccct tctggctgtg cttttgctaa gtgcaaatac atgggcagcc attgaaatca
    11341 ataaccacca ggccagaaat atggacgata tgcaaagctt aggcgtgatt tatatcaatc
    11401 ataatttcgc cactgaaagt gaagcacgtc aggcattaaa tgaagaaaca gatgcgcagg
    11461 gcgcaacgta ctaccacgta attctgatgc gggaaccggg gagtaacggc aatatgcacg
    11521 ccagcgcgga tatttatcgc tagcaccagg tataaccaac gaaacattgc catagtttgc
    11581 tttgccccct tcgcaggggg ctttttttga aaaacggctg cggaattaga ccagttatct
    11641 cccgaagaag gaaatttccg cagcgcgtgt tttacttctg cccgtaataa gcgttaggac
    11701 catgcttacg catgaagtgt ttattcatca ggaagctgtc gatgtgattg agttgtggat
    11761 taatactgcg cgcaatccac gccattttcg ccacttcttc catcactacc gcgttatgca
    11821 ccgcgtcgtg ggcatctttc ccccaggcga acggtccgtg ctgatacacc acaattcccg
    11881 gcgtatgcag cggctcggcg ttgcccagcg tttcgataat cactttgcca gtgttcagtt
    11941 catactcacc ctgcacctct tcttcgctta atccacgcgt acacgggatg tcgccaaaga
    12001 agtagtcggc atgcgtagtg cctaacgccg ggatcgccag ccccgcctgc gcccatgcgg
    12061 tagcatgagt ggagtgggta tggacaatgc cgccaagcga cggataacga cggtagagtt
    12121 caagatgcgt cgcggtgtcg gaagacggac gatattcccc ttccaccacc ttaccgctca
    12181 tatcgaccac caccatatcg tctgctttca tggtttcgta ggcgacgccg ctgggcttga
    12241 tcaccaccag cccgcgttcg cggtcgatag cgctgacgtt gccccaggta aaggtcacca
    12301 gcccgtagcg cggcagatcc atgttggctt caaatacctg ctgttttagc ttttgcatta
    12361 tgccgcctcc accatgcccg ctttcgccat gcgcgctttc acccaatcac gcgctttcgc
    12421 cacttctgcc gccgggtctt ccgccgtttc gctccacatc tcaatcaggt acggcccgca
    12481 atagccactc tgtttgagcg tttcgaaaca acgttcgaaa tccactacac cttcgccaaa
    12541 cggcacgttt ttgaagacgc caggtttggt gtctttcaca tgtaccgcga cgatatgccc
    12601 gattccggcc tgcaactcca tctgcacatc gttgtcccac gccgacaggt tgccgatatc
    12661 cgggtagagc tggaaccacg ggttgttgag atagtgcgcg taacccagcg ccttgctgat
    12721 ggagttcatc aacggataat ccatgatctc catcgccagc gtcacctgcg cgcggctcgc
    12781 catctcaacg ctctctttca ggccgtcacg gaaacgacga cgcgtttcgt tattggcttc
    12841 ctgatagtaa acgtcatagc ccgccagctg gatcacgcga atgccgacgt cctgggcgaa
    12901 ctggatagct ttacgcataa tctccagccc ctgcgcccgc accgcgtcat cttcactgcc
    12961 cagcgggaaa cgacgatgag cagaaaggca catggacggc acgcgcacgc cggtttcaac
    13021 aatcgcattc accagcgcca gacgctgctc gcggctccag tcgaggcgcg acaggcgctc
    13081 gtccgtttca tctaccgaca tctcgacaaa atcgaagccc aacgtttttg ccagttgcag
    13141 gcgttccagc cagcactccc cggcggggag cgctttttca tagatgccaa gcgggatttg
    13201 tttggacaac atatccgctc cttagcccca cagttcggcg atggaacgtt tgaactgacg
    13261 cgcggcttca accggagaag cggcatcacg gatgctgcga ccggcgataa acacatgaat
    13321 tggaatgcct ttgaacagcg gcagatcttc cagcgccagg ccaccggtaa cggtgacttt
    13381 gaagcccata tcggaaagac gtttgatcgc agtgatatcg gcttcacccc acgccacgcc
    13441 agcggcctgc gcgtcacggc tgcggtgata aaccacctgc tgaatacctg cgtcgcgcca
    13501 ctgttgcgcc tgttcccagg tccagtaacc ggtcagttcg atctgcacgt cgccgttaaa
    13561 ctcttttgct acgtccagcg cacctttggc ggtgttgata tccgcacaac aaatcacggt
    13621 cacccagtca gcgttggctt cgaagcacat acgagaaaga attttgcccg catcggcaat
    13681 tttggcgtct gccagcacga ttttgtgcgg gtagagcgct ttcagatcgc gaaccgcacg
    13741 cacgccttcg cccacgcaca gaatggtacc cacttcgata atgtcgactt cttcggcaat
    13801 caggcgagtg gtttcgtagg cgctatccat agtctggttg tccagcgcga cttgcaacat
    13861 cggtaatgac atcttcaatt ccttcttaag ctgccgcgtt ggtgcggtca atgagatcca
    13921 gtacttcctg ctcggtacgg caggcacgta aacggtcgaa attttcctca tcttcaaaca
    13981 ggttgacgat ctgcatgatg cccacttcct ggtgcgtgtt ggcatcgacc gccgccatgg
    14041 tgatgaggat atccaccggg tcgttatctt cgtggttgaa ctccagcggc tttttcagcg
    14101 tcaccagcgc aaaaccggtt ttcttaacac cctcttccgg gcgaccgtgc ggcattgcca
    14161 ggccaggtgc gagcacaaag tacggaccgt gctgttcaac cgcatccaga atcgcctggt
    14221 agtaacgcgg ctcgacaaca tccgccgcca ccagcaggtc aacgccgatt tttactgcgt
    14281 cctgccatgt ctcagcttca gcctgcaggc ggatggattt attttccgcc agcgaatcac
    14341 gtaatttcat ggcgcgtcct tacttcacat cctgcgggaa atgctctttg atcacttcca
    14401 gcagtttcgg gccaaaatcg gcaggagaga gcatgttgcg cacgccaacc acgtatttgt
    14461 tgccggtcac ggagatctcg cccgcgatgt gcgtggaagc gatgataata tccgcgttgc
    14521 tcagctcact tttgtactcg ccaaccgcgc agctgtttac cgtgtggtca atctttgatt
    14581 gggttaaaaa ctggtccact ttcattttca tgatcatgga actaccttgt ccgttgccac
    14641 acacagccag aatacgtacg gtcataatca aaactcctta ttaagcagac tgttctgcca
    14701 gttgtttttc tgcatcttct tcagcgcgca gtgcgcggcc agcgaagaac atataagcca
    14761 gtgcaattac aatgatgacg gccataaagg cgataccgat ggagaagaag ccctgcatca
    14821 tcggcggtgc cagaatcgac cagtccgcca tgcccatcca ggcgctcata ccggtgagtt
    14881 tcaccgccca gacgcagcca aagatttcaa tcatccccat caccagacat atcttcagcg
    14941 ccgcacgcca gccgccgaag tggttagcga acacgccgat ggtggcgttg gagaagaaca
    15001 tcgggataaa gccgggaata atcaggatcg aggaaccgca ggcgaccagg atgccaaccg
    15061 caatcagctg accgatggtg ccccacataa agccccaaac cacggcgttc ggcgcgaagc
    15121 tatagatagc cgcacagtca atcgccagaa ccgcaccagg gatcaggcgc tgggagatac
    15181 cgttaaatgc ttcagagagt tccgccacaa acatgcgcac gccctgagtg atgatgaaga
    15241 tcgccaccgc gaaggagaaa ccagtttgca ggatgtacac cgtccagttc actttgcctg
    15301 ccatcgcctg cacggtgtcg ataccgaagg agagcagaat ggcaccaaag aagatggtca
    15361 tcacaatcgc cgtggagacg atgttgtcgt ggaaaatatt cagccagcct ggcagtttga
    15421 ggtcttcaac gctttcctct tttttgccga ggaacggcgc aactttatag gcaatccatg
    15481 atgcaaactg ttgctggtga ccgatggaga aaccgcagcc gtccgtcact tcctgagtcg
    15541 gcttgtacat tatgttggag gtaatgcccc agtagagcga aaccagaatc gccgtgcaga
    15601 taatggtcgt ccacatggag tagccgaaaa taaacaacgt aacggcaatc agccccgcct
    15661 gctggaacat gatatgacca gtcagcatga tggtacgaat accggtaatg cgacgcagca
    15721 gcacgtaaca gatgttcaac gccagcgcta acaacaccgc gtaacctacc cagttatagg
    15781 catcgcccat gcggtcgatg gttgccatca tcgacgcgta agtatcggaa attgcgccgt
    15841 taatgccgta gacttcggac attttcgcca ccaccggttt gaaggtgctg gtgaggatgc
    15901 cggaccctgc ctgcaacaac atgaaaccaa ttatggtttt aatcgtgcct ttaataataa
    15961 cgctgacact tttgcgcagt aggatgtagc ccagacaggt cacaataccc agcaacaacg
    16021 gggcattggt catgacctgg ttaaaaaaca cggtaaagat gttgtagagg atctccataa
    16081 cgatctcctg aagaagaggt aaccgggtat tccacacacc cggaatgttg tgcactcact
    16141 ctaattttca aaagtaatca caacaagatt atttatgatt aaatgtgacg cgacccgcaa
    16201 ataattccac atggatagaa tgggattcac acaacgaaac aactattcat ttaaattcat
    16261 ttagttaatt gaattacaca ttccattaaa tctttccaca ctcaaaatca acaaccactt
    16321 ccctggcaaa aagtcaaaaa atggtcattg ccttcctgaa aaaatagtgt caggattaat
    16381 caaaaccaat cacatattga ttcgaattga actatcatga aggtaaatgg cgatgagtaa
    16441 agtgaaaagt atcacccgag aatcctggat cctgagcact ttcccggagt ggggtagctg
    16501 gctgaatgaa gaaattgaac aagagcaggt cgcctctggc acatttgcga tgtggtggct
    16561 aggctgcacc gggatctggt tgaaatcaga aggtggcacc aacgtttgcg ttgatttctg
    16621 gtgcggcact ggcaaacaaa gtcacggtaa cccgttaatg aaacagggtc accagatgca
    16681 gcgcatggct ggcgtgaaaa aactgcagcc aaacctacgc accaccccgt ttgttcttga
    16741 tccgtttgcg attcgccaga tcgacgcggt actggcgact cacgatcaca acgatcatat
    16801 cgacgttaac gtcgctgctg ccgtgatgca gaactgtgct gatgacgtac cattcattgg
    16861 accgaaaacc tgcgttgatt tgtggattgg ctggggcgta ccgaaagagc gttgcatcgt
    16921 ggtcaaaccg ggcgatgttg taaaagtgaa agacattgaa attcatgcgc ttgacgcttt
    16981 cgaccgtact gcactgatca ccctgcctgc cgatcaaaaa gcggctggcg tactgccgga
    17041 cggcatggac gatcgcgcgg tgaactatct gttcaaaacg cctggcggct ccctgtatca
    17101 cagcggcgac tcccactact ctaactatta tgcgaagcac ggtaacgaac atcagatcga
    17161 tgtggcgtta ggatcgtatg gtgagaaccc gcgcggtatt accgacaaaa tgaccagcgc
    17221 cgatatgctg cgtatgggtg aagccctgaa tgcgaaagtg gttatcccgt tccaccacga
    17281 catctggtca aacttccagg ccgatccgca agagatccgc gtgctgtggg agatgaaaaa
    17341 agatcgtctg aagtatggct tcaagccgtt tatctggcag gttggcggca aatttacctg
    17401 gccgctggat aaagacaact tcgagtacca ctatccgcgc ggtttcgatg attgcttcac
    17461 tattgaaccg gatctgccgt tcaagtcatt cctgtaatct gtcgtttata ccggatagcg
    17521 tggagaagtt cgcgctatcc ggcgtagtat ttcctggcta tttcaaatat catctaaaaa
    17581 aatcaaattt tatcggaata gctcatgact gaagcacaaa gacatcaaat cctcctggaa
    17641 atgctcgcac aattgggctt tgtgaccgtt gagaaagtcg ttgagcgtct gggaatttcg
    17701 cctgccactg cgcgacgcga tatcaataaa cttgacgaaa gcggcaaact gaaaaaggtg
    17761 cgcaatggcg cagaagctat tacccagcag cgcccgcgct ggacgccgat gaatctgcat
    17821 caggcgcaga atcacgatga aaaagtacgt atcgctaaag cagcctcgca gctggttaat
    17881 ccgggcgaaa gcgtagtcat caactgcggc tccaccgcgt ttctgcttgg gcgggaaatg
    17941 tgtggcaagc cagtgcaaat catcactaat tatctaccgc tggcaaatta cctgatcgat
    18001 caagaacatg acagcgtgat cattatgggt ggacagtaca acaaaagtca gtccatcact
    18061 ttaagcccgc agggcagcga aaacagtctc tatgccgggc actggatgtt taccagcgga
    18121 aaagggctga ccgcagaagg gttgtataaa accgatatgc tgacagcaat ggcagagcag
    18181 aagatgctga gcgtggtagg gaaactggtg gtactggttg atagcagtaa gattggcgaa
    18241 cgcgcgggaa tgctttttag ccgtgccgat caaatcgata tgctgatcac cggcaaaaat
    18301 gctaacccgg aaatcctgca acaactggaa gcgcaagggg tcagcattct gcgtgtttaa
    18361 agatgctggc ggaaaaatgt cacggcagca tctaacgcct caggcgtaat gcggtgacgc
    18421 acgcctggct gccatgaaca ggttagcagt ttatcccgtc ccgtctcgct taaggcctgc
    18481 tgcaaacgta gtgattcgtc ggcaggcaca acgtcatcgt cgaggccatg ccacagcagt
    18541 agaggtctgt ccccaagttg ttccaggtgg tttgtcgctt cccactctgc cagcggcgcg
    18601 ataatgttat tgaattcatt ctgctgtgct gccgtttcag gtatcagcgg tggaaacagt
    18661 gaacgggcga gcgatgtaaa atagcccgat cccatcatgc tggcggtaca tttcaccgtg
    18721 gggtggcgag cggtaattcc cagtgccgtc atcgcgccca tcgacgcgcc accgactgcc
    18781 agacggtcat caagcagcca gttttcttcg gctattgccg cacgtaaagt agtgaattcc
    18841 tgcatacttt gtagcaagat ttgccagaat tgatttaacc gccgcgcttc gtcgctacta
    18901 aaacggctac cgtgatcggg cgcatccggc atgatcaccc gcaaaccagc ctgcgccagc
    18961 gcaacggcaa aatagctata caccaggctg gatgaagtaa agccgtgata aaaaattacg
    19021 cacggtaacg gggtatcttt ttgcccgaca ggataagcat gaagaacggg aatatctgcc
    19081 agctcgcgtg attctatttc aatcatctgt ctctcctttt tgtctggtat gacatgaaga
    19141 aatccttagc acaagtttct gctggcggta ttagttcata atgttgagat gtgggttacg
    19201 ctttcgtcac gttttcattc gaaaatcgcg taccaggtaa caattcggga acattccccc
    19261 aaagttaaat taataaggca ctacactatg gttagcagga aacgtaatag cgttatttac
    19321 cggtttgcca gtttattatt ggtgttgatg ttaagtgcct gtagcgcact gcaaggtacg
    19381 ccacagccag caccgccagt aacgaaccat ccgcaagaga ttcgccgcga ccagacgcaa
    19441 gggttacagc gaataggtag cgtaagcacg atggttcggg gttctccgga tgacgcatta
    19501 gcagaaatca gagcaaaagc tgtcgctgca aaagctgatt attacgttgt cgtcatggtg
    19561 gacgaaacca tcgtgacagg acagtggtat tcacaagcca ttttgtaccg taaataaatc
    19621 agacaactta catagtcagc tttacattgc tttgcgtccc tatgcgttta cctgtgcaca
    19681 ataaattacc ggctggaagc ctgggcgttt attaccgcga tggaatgccc tgcaacatgt
    19741 ggggaaacga aaaatggagc tgacgatgaa acaattactt gcctcaccct cgctgcaatt
    19801 agtgacttat cctgcgagcg ccacggcaca gtctgcagaa ttcgccagtg ctgactgcgt
    19861 gacgggcctg aatgaaatag gtcagatctc agttagcaat atttcagggg atccgcagga
    19921 cgtggaacgt atcgtggcgt taaaggccga tgaacaaggt gcgtcatggt accgcattat
    19981 taccatgtac gaagaccagc agcctgataa ctggcgcgta caggcgatac tgtatgcatg
    20041 agtgtcatct acagcaaatg aacaatgccg gatgcggcgc gagcgccttt tccggcctac
    20101 aattttcaca tatttcattg agttaatcga aaccagcgtc gcatcagtcg tatacgctta
    20161 cacacacact ccccccgtcg cccgcagcaa taaatcattc tggatctgct ctgacaaccg
    20221 tacgccgccg cgcgtatcta acatcacctg acaccacgcc tgtgccattg gcggagaggc
    20281 atatttcaac atttgcgcac cgcagcccag caggaatagc tgatgagtaa tctctcgccc
    20341 caattcttca gctggcttgc gcagctgctg ctgtaaacga cgaaccgcgc gatcaaaata
    20401 gcgatcctgc cctttcactt ccacaaacgc ttccgacaat aagtcgtata cgcccgcttg
    20461 cttattgaga acgcgcagca catccaggca cataatattg ccggaacctt cccaaatact
    20521 gtttaccggc atctcccggt aaagccgcgg cagctcgctc tcctcgcaat aaccaatgcc
    20581 gcccagcacc tccatcgctt cggccacaaa cggcatacct cttttgcaga tcacaaattt
    20641 tgccgcaggc gtaaataaac gcgcccacag ggcttctttg gcatcggcac gccggtccca
    20701 cgctcgcgca agacgaaaca gcaacgccgt ttgcccttca agctgaagtg ccatgcgact
    20761 taagacatga cgcataaggg gctgttggat caatggatta ccaaaaacat ggcgttgatg
    20821 tgcatgataa atcgccagcg aaaatgcacg gcgcatcatg gcatggctac ccagggcgca
    20881 atcaaaacgc gtcatcccac ccattttcag gatcagacga attccttccc cttcctgccc
    20941 caacaaccag ccaatggcat cctgaaactc cacttcgcag ctggcgttag agcgattacc
    21001 cagcttatct ttcagccgct cgaggcgaat cgcgttgcgt tgcccgtcag gcaaaaagcg
    21061 cggcacaaaa aagcaggaca aaccgcccgt agtctgcgcc agcaccagat gcgcatcgct
    21121 ttgcggcacc gagaaaaacc atttatgccc caccagccga taagagccat cttccaggcg
    21181 ctctgcgcgg gtggtgttgc tcatgacatc ggaaccgccc tgcttttccg tcattcccat
    21241 gccaatcaac aaaccgcgtt tttgcccacc tggcaataag tgagaatcgt agcgatcgct
    21301 cagcagcggc gtggtccagt cctgaaacgg cgcgggtaac atctgtaaca gcaatggcgt
    21361 ggcggcaaag gtcatggtta tcggacataa cgaccctgcc tcaacctgtg cgtgcaacat
    21421 aaaacgcgcc gcgcgcgcca caaatgcgcc ggagcgagcg tcttcttccc aggcaagatt
    21481 gtgcacccga ttggtacata gcgcctgcat cagcaggtgc caggcggggt gaaaacgcac
    21541 atcgtccaga cggcgtcctt gcgcatcgta gcgcaataat tcaggcggat tcacattcgc
    21601 cagccgcccc agttcaaggg attcagccgt tcctaactgc tgaccaatac tggctagaaa
    21661 atcgctatcc cagccagcac cttcacgcgt taccgcttcg cagagcgcgc catcagacag
    21721 gtataagttg ctgttattta atggtatagg ttgattaaaa acggtgtgag tttgccagtg
    21781 cactgtgtct ccctccatca atggcagtca ccattagtat ggtcactgcc atggattcat
    21841 gacagaaaga gattgctaaa acattcttat ccagcaatgt aatgcggaac gaaacgtgag
    21901 gtgtctttgg tgatcagtgt gttatcttca cggatcccca ttccgcacgc ttcatcatcg
    21961 acaatccagc taccgatgag tgtgtagcta tcgccaaatc gcggcagagg ttgaaacgcc
    22021 tggtagatca tcggttcatc ggcgtaatca ccatcagcgt ggtcaacgac gttattctga
    22081 ccgtcgaaaa tggtgacgtt tccgccttcg cgcgagtaga tcggtttacg cacatagctt
    22141 tcgccagcgg caatctgcga tttttcaccc tcaaaccagg atgcaagaag gttaggatga
    22201 ccagggaaga agcgccaaag cagcggcatt agccctttat tactcaagat acttttccat
    22261 aacggctcca cccattgctc gcgacgcttg cacagcagcg gaccgttatc gtcacgcatc
    22321 atccactcca gcggatacag cttaaatgca cgctggatga cattatcatc aagatcggtc
    22381 agtacaccgc cgacgcccaa accgagatct tcaatgtaga taaaccgcga ctcctgccct
    22441 gcctgctggg cgcagtcctg caagtacagc acggtagtcc tgtcttcatc ggtgtcctga
    22501 cagcagcaga aataaaacgg ctcccggcta taaagctcgc taaagcgtga aatcaggcgt
    22561 tcctgaatgg cattgtactg atcggcatca cgcggaataa tgccgctgcg ccgggcatcc
    22621 tccagccaca accactggaa ataagccgac tcatacaacg aggtcggcgt atcggcgttg
    22681 tactccagca gcttcaccgg cgcattgcca caccaggcaa aatccatccg gccatacagc
    22741 gaaggatcgc gtgcgcgcca gctttcggcg atcacatccc agtacaacgg cgggatcgcc
    22801 agttgcgtca ggatctcttc gtctttaacc gcgcgatcca ccacctcaag gcacatctga
    22861 tgcagttccg cagtcggttt ttcgatctgc tcttcaatct ggcgcagagt aaaacggtaa
    22921 gcccgactct cgtcccaata gatttcattg tcgatgatat gaaagtcgaa accgttgtta
    22981 gcggcgatct ggtccagatc ccgtcgcaca ggaacgttgt gtctcagcat gattagcctc
    23041 cccaatgccc acgggcgctg gaagaacgac catagccgcc gcgcgaaacg gttgacgctt
    23101 ttttggtggt aaagccgcct gaagagtaag actctttttt gccagaaccg gagcgccagg
    23161 agtaatcacc ggaagtgttg cgccagacgg ggcgggaagc aaatgaggat ccgccgctgt
    23221 tatagacgaa cggctcatcg cgatctttac gaataactcg gcttaacaaa aatccagaaa
    23281 cgaccgggat ccaactctgt tcaacattgt cgtagtagca attttcgtac ttagactgac
    23341 aattctgctg agtcatattc ttcggaacat cggcataaaa tgccgctttg gcgttgttcc
    23401 agccacgcgc gcaaatgtcg gcattattac cgtcatcaat acaatcctgc actgtcgaat
    23461 aaaacgttcc gtcgccgtcg ttatcgacat cgccactttc actccatcct ttgactatta
    23521 aaaaaacggc accgcccatt agcaccaacg ttaagtattt ttgtgcgtag cgattgcggc
    23581 gcggctcaaa aggattatgc ggtcttccaa tgcgactgat cgctccgtga ccaattttac
    23641 tgttatttct tgatttgcgt tttctggcca tgatcgtcca ttaccatgtc atacaagctg
    23701 cgttaaaaat accaccagcc agtgcggcgg ttcccataaa cattcctgct gcggtattgt
    23761 gattaataat tttttcgctt aatgcgggca tataaagtct gacaccagca aaaacgagta
    23821 actgaatcac cagtgcaatc ccgccccagg cgaaatagtc tggaatactc accgcattga
    23881 ttgccgcact ggataagggg atcacgtaac ccaacaatgt accgctgaat gccagtgacg
    23941 ccgcggtatt attgtttttg attaattgcc attcgttgtg cggtgtaatt ttggagtaga
    24001 taaacagaaa aataatcacc atagccacgc caataaaaaa ataggcacta aaggcaagaa
    24061 gtgaatccag tatatgcatt gtaaacgtcc ttgtaattaa ccaataatat gaagtgatgt
    24121 cagcgggata tcgacaccca acgcgcgcga aaataaccac tctggctcgc cgagatcgtt
    24181 aaaagattcc tcaccattta acagcaaata ttcgtaagta tcttcggtta cttggcgttg
    24241 gtagcccatg gtaaaattat ggacctccca tttggcatgg ttttgatttt ctactttttc
    24301 cagcatgtag acgggttcga tatttcccgg ttcttcgctg ttaaaaaatc gctgccagcg
    24361 tttttcctgc cagtttaaga tcattgcccc catcactttg gcgttgatcg cctcgcgcca
    24421 gtgactctct ttactgatac cgtagctctc ttcatagaca aagagcttga tatcatcaat
    24481 gtcatcaata tcttcgccgc cggtggtatt gatttgcaga aattcatcgc ctgaagtgta
    24541 gtagcggaaa atctgactac cgccgcccag atcgatgtgg ctgacagcgg ctaccgtaaa
    24601 ttcctcaccc ggcagcgcaa tcagcaattc atcttccagc aaacgaaacg ctaacgtgtc
    24661 gagcgtaaag ccactattga gatgaagtcc cagcggaccg cgagcgatag caggcttatt
    24721 atccttgcca aacagacgct ggaaaaaacc agacatattt tatccttaaa atagttgcca
    24781 gccttttagc gaggctggca aaaaaattac tcgccctgtt ggcgttgcag tcttgctaat
    24841 acatcctggg cgctactttt attgctaccg ccaattccgg cttccgccag cttttcatca
    24901 agatcgcgac cgtctgcgac tttttccaac tgtgcagcag catccaggcg agcctgacgt
    24961 tcggcctgac gcgtttgcag gcgttttaag gattctgccg ctgtcgaaac gctggaagat
    25021 gcgccaacgg tagaggttgt tacagcctgt tgtgcacgct gcatggcttc agtggcttta
    25081 acgacttcca tttgctgctc aaactgagcg atacgctgcg ctgtcgctgt aactgctttt
    25141 tccacgccat cacgggagac ttccagattc gacaacactt gctcttcagc ggtaatgaga
    25201 ttctcaaggc gcgcgatttc ttcagcaact tcgttaatca acgacggatt aacgttcttg
    25261 cttaacgctt ccagcgcacg ggcttccaga ctggctttgc gctcacgtaa atctttcagc
    25321 ttatcgtgac ttaatttcac ccgcgccagc agatcaacgc gagattttcc ggctttatcg
    25381 agttcagctt tagcgtcacg aatatgctgt tccagcatgc gcacgccctg ggtttcttca
    25441 atggattctt ccgcctggga gataaacgat ttccccagcg taaataaact ttttaaaatt
    25501 cccataaata cccgtccttg taattagtgt gaatattctt ctgtaatttc agccagatcc
    25561 aatgcgttat ctacctgcga ggttatctca agcaggatat cttcaagaga agatttaagc
    25621 gataacgccc cgaaaacaat gtaatactct tcctgttgca cgctggagat tcctaccgat
    25681 gacaacggca tcatcttctg atttcttaat aagaaggtat taaattcatc tggattgctg
    25741 atactactta ccggacaaat aaaggtttcg ataatcattt ggcgggaagt aaaaagaata
    25801 ttgatttgca aatcgccata atcattcatt ttaataatta atgcgctctc gctatttgtc
    25861 acatcaatat tttgttcagg tacagtttgc agcgccgtcg ccagcgccaa cggattccat
    25921 gtcatatatt attccatata gatttagttt aaatattaat aaaatgaata tttgcagtac
    25981 gtaattatct taccagctat agacaaaaaa aaaccatcca aatctggatg gcttttcata
    26041 attcagacga actagctgcg ctgacgaacc gcttcaaata agcaaattcc ggttgcaacc
    26101 gaaacgttca gggaagaaac acttcctgcc attgggatgc tgatcaactc atcgcaatgt
    26161 tcacgggtca ggcgacgcat accttcacct tccgcgccca tcaccagcgc caggcgtccg
    26221 gtcattttgc tctgatagag tgtatgatcc gcctcgcctg ccgtaccgac gatccagata
    26281 ttctcttcct gcaacatacg catggtgcgc gcaaggttag tcacccgaat cagtggaacg
    26341 ctttctgccg cgccgcaggc tacttttttc gccgtagcgt tgagctgtgc ggagcgatct
    26401 ttcggcacaa tcaccgcgtg aacgccagca gcgtccgcgc tacgcaggca cgcgccgagg
    26461 ttgtgcggat cggttacacc gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc
    26521 agatccggca gatcgttttc ctgatactga cgtcctggct tcacgcgggc gataatgccc
    26581 tgatgcacgg caccgtcgct tttctcgtcg agatattggc ggtttgccaa ctggataacc
    26641 acgccctgag actcaagggc gtgaatcagc ggtaacagac gtttatcttc acggcctttt
    26701 aaaataaaga cttcctgaaa acgttcaggg gcgcgctcca gcagggcctg cactgcgtgg
    26761 atgccgtaaa tcatttcgct cattaatgta ctcgttgttt acgttttgcc tgatgcacta
    26821 cgtttatccg gcctacatga tctctgcaat atattgaatt tgcgtgatta tgtaggccgg
    26881 ataaggcgtt cacgccgcat ccggcatgaa caaagcgcat tttgtcagca atctaaccct
    26941 cttcttttaa agagggtatt gatcactctg ccactttttt cttcgccgca cgcttcgctt
    27001 tggtcgctgc ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt
    27061 tcttcgccgc tttcggcttc gtttttttct caccgcggaa ggcgctgtct ggctcaaagt
    27121 ttaccttttt accgacctga cgacgcttgc cgcctttttt gcctgcatcg ccttttttcg
    27181 ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg ttcactggag atcagactaa
    27241 agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca
    27301 ggcgataagt ctggccgctg gattccccca tcaggcgttg ccctacctgg tcaaagcgat
    27361 agtagtcatt gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac
    27421 ggacgaagaa gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct
    27481 ggtcgagcat gaagtcacac ttcagccagt cagcgacatc gcgcgttgct tcgtcggcac
    27541 gacgttccgc catcgaacag tgctgaccca gttgcaacat ctcttccatc gaataatggt
    27601 agccgccggt ttcagtggtg ttgccctgat gcccctgctc tttcgacagc agatatttaa
    27661 tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg
    27721 actgcaatgc cagaccgaag tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg
    27781 agcgtagcag catggtttgc agcatttctg catcaggacg gtcggcaacc gactccagca
    27841 gttccgcgta gtcacgcggt tccggcttgt taccacccgg cagctccagc cccagctccg
    27901 ccagcactga acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca
    27961 gtgccggttc tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc
    28021 actcttcaat taatttgtgc gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc
    28081 gttcagcgtt gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt
    28141 cacgggcttt atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg
    28201 gggcgtactg ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg
    28261 tcagacgcgc gtgagagctc atcaccgctt cgtagaattt gtagcccgtc aggcggcctt
    28321 tcgacgaaac cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgaac
    28381 acaggccgtt agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg
    28441 acgtgccacg gttacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga
    28501 cgtcggcaat cgcgacccat aaacgccagc cgccgccgcg ttttttctcg cagtaaactg
    28561 catcgtcaaa gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat
    28621 cgacacggcc cgcttttgct tcttccggca cttcttcttt cagcccggca acctgttgct
    28681 caacagcctg cggccagatg tacggaattt catgggtacg cagagcgata tcaaccgcca
    28741 tgccggtgcc catattatcg ccaaggactt cgacgatttt acccaccgct ttggtgcggc
    28801 gagtcggacg ctgagtcagt tcgactacga ccacaaagcc catccgcgcg cccatgatct
    28861 gatcgggcgg gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga
    28921 cgcccgcttc ggtaaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga
    28981 caatacgcgc ttcacgacga cctttacggt cagcgcccag cggctgagcc agcacctgat
    29041 cgccatgaat gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt
    29101 caacccgcag aaagccgtag ccatcacggt ggccaataac ggtacctttc accaggtcga
    29161 ggcgttccgg cagcgcatag cactgacgac gagtgaagac cagttgacca tcgcgctcca
    29221 tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta
    29281 ccgccagctc atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt
    29341 cccgactagg gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg
    29401 acatctcggt tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa
    29461 tttataaagc ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt
    29521 aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg
    29581 gcaaaactca ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc
    29641 aatacgtatc gcactcgccg gtttacccag gcgaatgccg ccattttttc cacgaacagc
    29701 agtcacgtag ccggcacgac taagttgatt gattattttg accatatgat tacgggagac
    29761 gccgtagacg tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat
    29821 gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa
    29881 agggaaatcg ggaaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct
    29941 aatttatttc agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta
    30001 atggcttaac cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca
    30061 ggcgtaccag aattacgcgt cgaacggatc gcgcagaatc atggtttcag tacgatccgg
    30121 accggtagag atgatatcga tcggcacacc agtcagctct tcaatacgct tgatgtagtt
    30181 cagcgccgcc tgcggcagac cgctacgatc tttcacgccg aaggtggatt cagaccagcc
    30241 cggcatggtt tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt
    30301 cgtcacttca cgaccatccg gcatacggta agccacacag agtttcacct ctttcaggcc
    30361 atccagaacg tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg
    30421 acgaacggca acggtgtcca gccagccggt acgacgacga cgccccgtag ttgcgccgaa
    30481 ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca aacagttcgg tcgggaacgg
    30541 acctgcacct acacgagttg agtaagcttt gaggataccc agaacataat caacataacg
    30601 cgggcccagg ccggaaccgg tcgccacgcc accagcagtg gtgttggaag aagttacgta
    30661 cggataagta ccgtggtcga tatccagcag cgtaccctgc gcaccttcaa acatgacgaa
    30721 atcgccacgc tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag
    30781 gatgtcggca acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt
    30841 gtagtagtta accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa
    30901 ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga cgtgctactt tatcttcata
    30961 agcaggcccg ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg
    31021 cgcgttatcc agcgcaacgt gataatcaag gatcagcgga catgcttcag acagcagcag
    31081 acgctcacga acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcggc
    31141 cggagacagc acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga
    31201 tggaataaga tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg
    31261 accgccctgg tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc
    31321 tttaccttcg tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa
    31381 tcaccgtttg cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc
    31441 actcaaaaat gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt
    31501 agtagaccac aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg
    31561 gcaaattggt catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta
    31621 aaccttccag taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc
    31681 cttatagaaa aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac
    31741 cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc
    31801 tatccgggct catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca
    31861 ggctacggat gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg
    31921 ctgcttcggc atcgccttca ccacgcatga tgcggccctg acgctcagct tctgccagcg
    31981 ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg
    32041 aacggtgacg acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag
    32101 acacttcggt cggcaggttg atctgcttaa ttcgcacatc gacaacctca atacccagcg
    32161 ccgccatact gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct
    32221 ctgccgcttc ggcaatggcg ttatctgccg ccggggtagt aacttcatct tctgtacccg
    32281 cagaaccgga gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga
    32341 cgatatcttt cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt
    32401 tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag taacggctga
    32461 aatcgctgat gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca
    32521 caaagcggtc ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt
    32581 caatgaacgg tatcttgaaa tgcagacccg gctcataaac cagaggtttg ttgtcatcgt
    32641 cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga
    32701 cagacatgta aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg
    32761 ttattccccc tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat
    32821 atcgccctga ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg
    32881 cggcagacgc agcagattgc tggtaccgtt atcgctcttc gccgcagggg cgttaccgcc
    32941 tttcagcatc tggtctaacg gcagaaccat caggttgcca cctttatcgt taaccagcac
    33001 tttgcgggtg ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat
    33061 ttccggcgcg gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc
    33121 ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc
    33181 gttcgcacgc ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc
    33241 gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc
    33301 agcctggaag ttgacgtcca gcagcgtgat acccatgtca tacggacgga tagtctcttc
    33361 cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat
    33421 ggtgtattta ccgataaccc cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc
    33481 cgggctggtc acgctataca gatatttttc cggattggtg acgcggtact gcacgttcat
    33541 ctcaacgcgc actacgttct cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg
    33601 cacggcttcc acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc
    33661 cggctcaacc agatggctga atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt
    33721 aatggtatag aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac
    33781 gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt
    33841 gcctttaccg ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa
    33901 atcaggtggc ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg
    33961 tttgctgctt ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat
    34021 gtttgtgctc catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg
    34081 actccacgct tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt
    34141 tacagaggcg acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct
    34201 cctccatcca ctctttttct attgcctgaa gctgataaaa acgacttctc agacgccctt
    34261 cctgcggcgg caaacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag
    34321 cctgaaaaag ctgtggtatc cccgctccgg tctgtgcgga aagccagaca cggatcggtt
    34381 tgttctcttc gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca
    34441 tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt
    34501 cgatgttttc ttgtacacgc acatccgccg catcaatgac gtgcagcagt aatgtggctt
    34561 gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa
    34621 taaaccctac ggtatctgca agtacggttt caccgacatc tgcaacgtca atacgccgca
    34681 acgtcgggtc gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc
    34741 gattgaaaag ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt
    34801 cggctttgat acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgtg
    34861 actgtatctg cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac
    34921 ccggaccacg caaacctatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca
    34981 ggcgcgtagc cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac
    35041 gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca cgacactcgc
    35101 acaaacgctc caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg
    35161 aagcacccgt cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat
    35221 actttgggtg cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg
    35281 aagagaccag agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga
    35341 tgtgtaccag taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct
    35401 aaatagatca gcggggaacg caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa
    35461 ccttattcgg tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga
    35521 tggtagttac tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagac
    35581 gggacaacag tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg
    35641 atcacgaact gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa
    35701 atagaaactg gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc
    35761 cccttagcca ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg
    35821 aaaaaattgc gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac
    35881 ctgtaatact tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc
    35941 ccaaccacgc agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg
    36001 ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc
    36061 cacgcaacga atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac
    36121 ttctgcttca aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag
    36181 cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc
    36241 tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg
    36301 ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg
    36361 aagttgacga tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg
    36421 cacttccggg tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa
    36481 catcgtaccg cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc
    36541 cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag
    36601 caatcggtgc ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat
    36661 ccctttgtaa ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag
    36721 ctcaatggct aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg
    36781 caggctcgcc ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa
    36841 cagattgtaa cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt
    36901 ccgccagcag ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc
    36961 gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta
    37021 tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga
    37081 tggtcacatg ctgtgcatct gactggaaat caatacccaa ttccgctagg gcagactgcg
    37141 ctttttctaa tgccgatttt tcttcggcag aaacttttag ccgcaacgga atcagcagcg
    37201 gctgggcgca aacgggcgct tcacccggcg tcaattgtgc ctgacgtagc caacgttctg
    37261 ccactggcaa ggataaaagt gaaatgttgc catcgcgctc cagcaacgca cagtcggaat
    37321 ggacgatagt cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg
    37381 gttccggcgc ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt
    37441 caccttgctg tttctggtag cctggctgcg cattcggcca gggggcagcc ggacgactgc
    37501 ctgatgctgg cgcaggagtg tagcgcggag ctaccggctc acgagctgcc ggttctgcaa
    37561 agtgattgcg ccccgccgcc acgcggtttt ccggaatgga acgcggtgca ggttggggtt
    37621 catcgtccag cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat
    37681 agataaaatc atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca
    37741 cgttgacgtc cacctgatgc ggatcgatct ccagatacag cacaaatgcc ggttgctgat
    37801 cggcccccag tttgtcttcg caggcctggc ggatcgcgtg attgatcagg cgatcgcgca
    37861 tcatgcgacc gttcacgtag caatactgaa tttctgccag tgcgggcgtg gtgtgatttg
    37921 gatcggccac ccagccgcgt agcgtgagat cgccgtgttg ccattcaatc gccagcgctt
    37981 gttcaagaaa agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg
    38041 gcactgcgcg gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga
    38101 aacgcgccag cgcaatgcgg cggatgatct catcaatgtg gttaaattcg gttttctcgg
    38161 tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg
    38221 tccccacagg atgcgccgcc ggttttaccg tcacgttcat atcgcgccct tcggcatagg
    38281 cctgccaggc ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac
    38341 tgatactcgc cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca
    38401 gagaggcgat tttactggtg gcatgacgcg ccagcgccag cgccagctcg tcttttttga
    38461 taccgcagcc gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat
    38521 cgatacgtgt cgcacctgca tcgaggctgt tttccaccag ttctttgact accgacgcag
    38581 gtcgctcgac cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa
    38641 ttggcatcaa tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg
    38701 tttgtgccgt tgccccctgc ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca
    38761 ggcctttgta aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt
    38821 cttcgctgtt gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgaac
    38881 gcagaacgcc gagactcgcg tgttctggtc ggcgtttatg tatttcgcca atgcgttgca
    38941 actgactgat catactggtc gctacatcat accctacccg ttgggaatga ccgaactgta
    39001 aatccagcac cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg
    39061 ccccacccag cagctccgac tgtttctcgt gctgctccag ccagctggcc atttcgctgt
    39121 tggcgcgacg gttagagagc acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg
    39181 catcagcgtg aatcgacacg aggaaattgg cgttttgctt acgtgccaca tcactgcgcc
    39241 ccatcaccga gataaagtaa tccccgtcac gggttaaaac gcccttaaac atcggatcgt
    39301 cattgagcaa agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac
    39361 caccggggcc gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa
    39421 ttttatcgcc agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga
    39481 taacacccgt agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc
    39541 gcggtgcgac aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggc ggaggaggag
    39601 gtggcggcgc atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct
    39661 tcaccgcttc ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat
    39721 ctttaggcgt tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca
    39781 gtccctgaat cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc
    39841 taaacgcata atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt
    39901 tagaaacctg aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg
    39961 tcgctaccaa ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg
    40021 ctaaacgcgc cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc
    40081 cttgtgcctg ataatcaatg tgtatttcga catccgggtc aggaagaaca ccagtacctt
    40141 gttgtggcca ctccaccagg cagatggcat cgttggcaaa ataatcgcga atccccataa
    40201 actccagctc ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc attaaattgt
    40261 cgagcgtata gggttcgacc agcgtataag tggggctttt gacattaccc tgatgaccca
    40321 gagcttgtaa aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat
    40381 agattacggt tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct
    40441 gctcatcagg gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta
    40501 acaatacgct gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc
    40561 gccgccagta cgtcagctgc agcaccgtgc gcgacacagc ccgcacaggc ggcatcatac
    40621 ggcgacagtt tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg
    40681 ccgctcgcca tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg
    40741 accacggttc cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg
    40801 gcgcaatgta agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc
    40861 tcgccaggat gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg
    40921 ttcaatgcat cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct
    40981 tttttccccc actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc
    41041 aggctttcgg taagagagtc catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc
    41101 agcagcggcg caatgttttc actgcgggtc agtactcgga ctaaaccagc accagcacgt
    41161 agcgccgctt cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatt
    41221 accagccgcc cgtgatcgcc tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa
    41281 agttgttctg ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca actatccagc
    41341 cccagtgagt caaaatgcag ttgtccggta acatcccgcg cttttccagt gagcaagcct
    41401 ggtttcagcg caataaaagt gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg
    41461 ccggtttcag ccagcaggcc ggaagggata tcaaccgccg caatcggcgc aggatgggaa
    41521 ttagcgtggt cgattaactg gctaatggat tcgcggggcg cttgctgcaa gccggtaccg
    41581 agcagcgcat caacaatcag atctaccgat tcgggccaga caatattcga agcatggatc
    41641 tcgcctcccg cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg
    41701 tcgctctcct gggccagcag cgtgacctca atgccgaccg ctgtggccag ccgcgcgacc
    41761 acgtagccat cgccgccgtt attaccatga ccgcacagca ccagccaatg gcgggcgtca
    41821 ggatacgccg aacgacacac ctggaatgcg gcttcgccag cgcgaagcat cagctcatag
    41881 agtgtaagcc ccagcgcatc tgccgcctca cgttctccgc ggcggatatc gtcggcgtgc
    41941 cagacggtgt gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag
    42001 cccctcgatc tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt
    42061 cagcaggtag gtattaccga taccgatctc agcgagtccg agcccaaact gcaagcatgg
    42121 ctggacaaac aataccacgg cgaaatggac tggatggcac gtcacggtat gctgcgcgct
    42181 cgcccccatg agttattgcc cggtacgctg cgcgtgatca gcgtgcggat gaattacctt
    42241 cctgctaacg ccgcatttgc cagcacgctg aaaaacccca aactcggcta tgttagccgt
    42301 tatgcgctgg gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag
    42361 atgattcagc aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt
    42421 ctcgagcgcc cgttagctga aaaagctggg ctcggctgga caggtaagca ctcacttatc
    42481 ctcaatcgcg aggccggttc tttcttcttt ttaggcgaat tgctggtcga tattccgctg
    42541 cccgtggatc aaccagtcga ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc
    42601 ccgaccggtg ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc
    42661 accatcgaac ttgaaggggc gatcccggaa gagttgcgac cgttaatggg aaaccgtatt
    42721 tacggttgcg atgactgcca gcttatctgc ccgtggaatc gctattcaca actcaccaca
    42781 gaagaggatt tcagcccgcg taagccgcta cacgcaccgg aactcattga gttattcgcc
    42841 tggagcgaag agaagttttt aaaagtcacg gaaggatcgg cgattcgtcg tattggtcac
    42901 ctgcgttggc tgcgtaatat cgccgtagca ttaggcaatg ccccttggga tgaaacgatt
    42961 ttgacagcgc tggaaagtcg taaaggtgag cacccacttc tcgatgagca catagcgtgg
    43021 gcgattgcgc agcaaataga gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa
    43081 aaacagcgtc tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac
    43141 agcttgtgaa taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat
    43201 cgactcgaca aaacggaatc ttaattttta acttatctat caataggtta aaaaaatact
    43261 attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg
    43321 tttacaagag tttgtcagag acaacgaaaa ccagcaataa cgcatcgcat cgctgtggat
    43381 attttattga gagaagaatt tggagcggga aacgagactc gaactcgcga ccccgacctt
    43441 ggcaaggtcg tgctctacca actgagctat tcccgcttgg gtggtctgtg ccttacagca
    43501 ctttcaaatt ttggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc
    43561 gtgctctacc aactgagcta ttcccgcttg ggcggtctgt gctttgcagc acttttcaaa
    43621 ttttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
    43681 ccaactgagc tattcccgcg tactacttaa ttttgctttc gtaattttcg catttctgcg
    43741 tcgttacggg aggcgcatta tacgagaaat ctttccctct gcaacccccc aaaaaagttt
    43801 tttttgcgtt tttatctcaa gtgctgaaat tatcagcaag acgttcaatt tagcgtcaaa
    43861 atcacgccgc cgcaagtgga ttccggcgct tacgtgacca ggaaaaattt tacagcttga
    43921 taaaatgctc gcggtagtaa gccagctccg ccaccgattc acggatatca tccatcgcct
    43981 gatgcgtccc ctgcttggta aaaccatcca gaatttccgg cttccagcgg cgcgccagct
    44041 ctttcagggt gctgacatcg agataacggt agtggaagta ggcttccagc tccggcatgt
    44101 atttaaacag gaaacgacgg tcctgaccga tgctgttacc gcaaatcggc gattttcccg
    44161 caggcaccca ctgttttaaa aattcgagcg ttgccagttc agcttcccga tcgcccatcg
    44221 tgctcgcttt cacgcgctct accagcccgc tggcggtatg ggtgcgcacg ttccagtcat
    44281 ccatcagcgc cagctgttca tcagactggt gtactgcaat ggtcggccct tctgccagaa
    44341 tattcaggtt ggcatcggtc accagcgtgg caatctcaat aatgcgatcg cgctcgggat
    44401 ccagaccggt catctcaaga tcgatccaaa tcaggttgtt ttcattggca ctcatgctat
    44461 tttccaccca ttcacagtga ctatattgat ccacaattgc gtgtatcata gatgttttgc
    44521 ccatcagggg cgaccaggag tctgtacgat tgagtaaaaa taaactctcc aaaggccagc
    44581 agcgccgcgt gaacgccaat caccagcgtc gtcttaaaac gtctaaggag aagcccgact
    44641 acgacgacaa tctgtttggc gagcctgatg aaggtatcgt catcagccgc tttggtatgc
    44701 acgctgatgt ggaatccgcc gatggcgacg ttcaccgctg caatattcgc cgtaccatcc
    44761 gttcgctggt aaccggcgac cgcgtagtct ggcgtccggg taaaccggcg gcggaaggcg
    44821 taaatgtcaa agggatcgtg gaagcggtgc atgagcgtac ctcggtgttg acgcgtccgg
    44881 atttctacga cggcgtaaaa cctattgccg ccaacatcga ccagattgtc attgtctccg
    44941 ccattttgcc ggagctgtcg ctcaatatta tcgaccgtta cctggtggcc tgcgaaacct
    45001 tgcagattga gccgattatt gtgctcaaca agatagacct gctggacgac gaaggcatgg
    45061 cgttcgtcaa cgagcagatg gatatctacc gcaatatcgg ttatcgcgta ttgatggttt
    45121 ccagccatac tcaggatggg ctaaaaccgc tggaagaggc gttgaccggg cgcatcagca
    45181 tttttgccgg gcagtctggc gtcggcaaat ccagcctgct gaatgcgttg ctggggctgc
    45241 aaaaagagat cctgaccaac gatgtctctg acaactcggg gctcggtcag cacaccacga
    45301 ccgccgctcg gctgtatcac ttcccgcacg gcggtgatgt gattgattcc cctggtgtgc
    45361 gtgagttcgg cctctggcac ctggagccgg aacaaatcac tcagggcttt gtcgaattcc
    45421 atgactattt aggtctgtgt aaatatcgcg attgcaaaca cgataccgat ccgggctgcg
    45481 ctatccggga agcggttgag gaagggaaaa tcgcggaaac ccgtttcgaa aactatcacc
    45541 gtattctgga aagcatggcg caggtaaaaa cgcgtaaaaa cttttctgat acggatgact
    45601 gacaattaag ctaaccgtcg ttagaatcgt cccctttttt tcaggatccg gcatgttggc
    45661 cggatcagga acgacaaaac aatggcctgg aggctacctt gttaaattca tttaaacttt
    45721 cgctacagta cattctgccg aaactatggc ttactcgcct ggcgggttgg ggcgcaagca
    45781 agcgggcagg atggctgaca aaactggtta tcgatctgtt cgttaaatac tacaaggtcg
    45841 acatgaaaga ggcgcaaaag ccggacaccg ccagctaccg cacctttaac gaattctttg
    45901 tccgtccgct gcgtgacgaa gtacgcccaa tcgataccga tccgaatgtc ctggtcatgc
    45961 ctgccgatgg cgttatcagc cagttgggta aaatcgaaga aaataaaatc ctgcaagcca
    46021 aaggccacaa ctacagcctc gaagccctgc tggcaggcaa ctatctgatg gcggacctgt
    46081 tccgcaacgg tacgtttgtg accacttacc tctccccgcg tgactaccat cgcgtacaca
    46141 tgccgtgcaa cggtattctg cgtgagatga tctacgtgcc gggcgatctc ttctccgtta
    46201 accatctcac cgctcagaac gtgccgaatc tgtttgcccg taacgaacgc gtgatttgcc
    46261 tgttcgatac cgaatttggc ccgatggcgc agattctggt cggggcgacg attgtaggca
    46321 gcattgagac ggtctgggcg ggcaccatta cgccgccgcg cgaaggtatc atcaagcgct
    46381 ggacctggcc tgccggggaa aacgacggtt ctgtggcact gctgaaaggc caggaaatgg
    46441 gccgctttaa actcggctct accgttatca acctgtttgc accgggtaaa gtgaatctgg
    46501 ttgagcaact ggaaagcctg tctgtcacga aaattggcca gccgctggca gtatctaccg
    46561 aaacctttgt tacgccagac gctgaacccg ccccacttcc tgctgaagag atcgaagcag
    46621 aacacgacgc cagcccattg gttgacgata aaaaagacca ggtctaatcc atcaaaggaa
    46681 acgctgacgt gcgcctgatt atcacttttc tgatggcctg gtgcctcagt tggggggcgt
    46741 acgccgcgac ggcccccgat agcaaacaaa tcactcagga actggagcag gcaaaagcgg
    46801 cgaaacccgc acagccggaa gtcgtagagg cgctccagtc tgccttaaat gcgcttgagg
    46861 aacgaaaagg ttcccttgag cgcatcaaac aatatcagca agttatcgat aattatccga
    46921 aactctccgc tactctgcgc gcacaattaa acaacatgcg tgacgagccg cgcagcgtgt
    46981 cgccgggaat gtctaccgac gcgctgaatc aggaaattct ccaggtcagc agccagttgc
    47041 tggataaaag ccgtcaggcc cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc
    47101 tgaatcaact gccgcaacag caaaccgacg cccgccgtca gttaaatgag atcgagcgcc
    47161 gcctgggaac gcttactggc aataccccgc tcaatcaggc acaaaatttc gcgttgcagt
    47221 ctgactccgc acgcctgaaa gcgctcgttg atgaactgga actggcgcag ctgtctgcca
    47281 ataaccgtca ggaattagcg cgcttacgct cagagctggc ggaaaaagag agccagcaac
    47341 tggatgcgta tttgcaggcc ttacgtaatc agttgaacag ccaacgtcag cttgaggcgg
    47401 agcgggcgct ggaaagtacc gaattgctgg cagaaaacag cgccgatttg ccgaaagaca
    47461 tcgtcgcgca attcaaaatt aaccgcgaac tatcggcggc actgaatcaa caggcgcagc
    47521 ggatggatct cgttgcctcg caacagcgcc aggccaccag ccagacgtta caggttcggc
    47581 aggcgctgaa tacgctgcgt gaacagtcgc aatggctggg atcgtccaac ctgctcggcg
    47641 aagcgttgcg ggcgcaggtg gcacggctgc cggaaatgcc gaaaccacaa cagcttgata
    47701 ccgaaatggc gcagttgcgt gtgcaacggt tacgttatga ggatctgctt aataaacagc
    47761 cgctgctacg gcaaattcat caggccgacg gtcagccgct gaccgccgag caaaaccgta
    47821 ttctggaagc acaactgcgc actcagcgtg agttgctgaa ctcattgttg cagggtggcg
    47881 acacgctact actggaactg accaagctga aagtctccaa cgggcaactg gaggatgcgc
    47941 tgaaagaggt gaacgaagca acgcaccgct atctgttctg gacctctgac gtgcgcccga
    48001 tgaccatcgc ctggccgctg gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca
    48061 ccttcagtca gttgggcaaa gccagtgtga tgatgctgac cagcaaagag acgattttgc
    48121 cgctgtttgg cgcgttgatt ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc
    48181 gttttcttga acgttcggcg gcgaaagtcg gcaaagtgac tcaggatcac ttctggctga
    48241 cgttgcgcac tcttttctgg tcgattctcg tcgcgtcacc gctgccggtg ctgtggatga
    48301 cgctgggtta cggcttgcgc gaggcgtggc cttatccgct ggcggtcgcg attggcgatg
    48361 gcgtaacggc caccgtgccg ctgctgtggg tagtgatgat ttgcgccacc tttgcccgcc
    48421 cgaacggctt gtttatcgct cattttggct ggccgcgcga acgtgtttcc cgtgggatgc
    48481 gctactacct gatgagcatc gggcttattg tgccgctgat tatggcgctg atgatgttcg
    48541 ataacctcga cgaccgtgaa ttctccggtt cgctgggacg gctttgcttt atcctcattt
    48601 gcggtgcgct ggcggtggtc accctcagcc tgaaaaaggc cgggatcccg ctgtacctca
    48661 acaaagaggg cagcggcgac aacattacca accatatgct gtggaacatg atgattggcg
    48721 caccactggt tgccattctg gcgtcagcgg tgggttatct ggcaacggcg caggcactgt
    48781 tagcgaggct tgaaacctcg gttgccatct ggttcctgct actggtggtt tatcacgtta
    48841 tccgccgctg gatgctgatc cagcgtcgca ggctggcgtt tgaccgggcg aagcatcgcc
    48901 gggcagagat gttagcgcaa cgcgcgcgtg gcgaagagga agcgcatcat cacagtagcc
    48961 cggaaggggc aattgaagtc gatgaaagcg aagtcgatct cgatgccatc agtgcgcaat
    49021 ccttgcggct ggtgcgctca attttgatgt tgatcgccct gctttctgtc attgtgctgt
    49081 ggtcagaaat ccattccgct ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcacct
    49141 ccacggtaca gggcgtagaa agtctggagc caattaccct cggtgcggtg ctgattgcca
    49201 ttctggtgtt tatcatcacc acgcagctgg tgcgcaacct gcccgcgctg ctggaactgg
    49261 cgattttgca gcacctggat ttaacgccgg gtacgggcta cgccatcacc accatcacca
    49321 aatatctgct gatgctgatt ggcgggctgg tcggcttctc gatgattggt attgagtggt
    49381 cgaaattgca gtggctggtt gccgcgctcg gtgttggtct cggttttggt ttgcaggaga
    49441 ttttcgccaa ctttatctct ggcctgatta tcctgttcga aaaaccgatt cgcattggcg
    49501 atacggtaac gatccgcgat cttaccggta gcgtgacgaa aattaacacc cgcgccacca
    49561 ccatcagcga ctgggaccgt aaagagataa tcgtgccgaa caaggcgttt attaccgagc
    49621 agtttatcaa ctggtcgctc tctgactcgg tcacgcgcgt ggtgttgacg ataccggccc
    49681 ctgccgatgc caatagcgaa gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct
    49741 cgctggtgat cgacaacccg gcaccggaag tcttcctggt ggatctgcaa caggggattc
    49801 agattttcga gctgcgtatt tacgccgctg agatgggtca ccgtatgccg atacgccatg
    49861 agatccacca gctgattctg gcaggcttcc acgcccacgg tatcgatatg ccattcccgc
    49921 ccttccagat gcgtctggaa agcctcaacg gtaagcaaac ggggagaaca ttaacttctg
    49981 cgggcaaagg tcgtcaggcg ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg
    50041 tggctcattt agcagcttaa catcatcttc agttatcacc ggccctggag agtatcatcc
    50101 atacgcatta cttcctgaca actcatcgcc ggagagacga tgagttgcag gatttcatat
    50161 gatcagtcga gagctaactc aggtttattc gccatgcgac tgcaatagtt ctggtaaatc
    50221 gccatcgcta acagcgagaa gaagacaggg ccgccaatca tccacaatgt gctgtcccag
    50281 tctccggctt caaccacagg ttgaatgatg gtgaagacgt tcgcaaatgt caccaccagt
    50341 acgacaacca ccgtcgcaat cattgccgac atacgcgttt tgaaaatcac aaatggtctg
    50401 tcgagatcct gacgtgcttt aaagaatggg aaagccagcg cgaggaacag gtaaggaagc
    50461 gtcatagaca cgttcgccat cagcgtcagc ttgttaaaga acgccgatgc ggtaccgcca
    50521 ccaaacgaaa ccagcaggat gaagacagta accaacccgc actgcatcca catggcgata
    50581 gaaggcatcc ccatcgcatt caggcgcgtc atcggttccg gccacaacgc tttcggcgtc
    50641 ccctggatga tggctttcag cggtgaatag caaagcgtaa agaacgcccc ggtataagcg
    50701 aggaacatcg aaagtccggt aatacgcgca aaccatacac ccagcgacaa tgacgcttca
    50761 ggtgacaaat gcagtgcgtt acccagcgtc accccgaggc tcttcatcag cacataggta
    50821 atattgccga ggttaacgga accattactt aatacctgct gccagtttgt gctgacaccc
    50881 cataaaaata ttgccagcga ataaccgatt gaaataacaa tagcggcaaa aacaatacct
    50941 ttggcaaagt tcttttctgg attttccgtt ttatcgacca gaccaccaac cgcttcaatt
    51001 ccgccatagg caaaaatagc aaataccaca aacgatagca ttgccagacc ggactgataa
    51061 cctgggttcg gtgatgcaag gaaattaata tcctgcgcga aatgcccacc atttaataac
    51121 aaaatagtaa tgcttactaa cagcaatact aaattcagac acattactgc aataccgccc
    51181 accgcagtaa tgcgggcaat tttattaatc ccctttgaag caacgacggt gaccagaatc
    51241 atccatgcta ctgccagcag accaaccacc tgcgtaggct ccagtccggc aatacgccag
    51301 tgctgggtca tgtcgctacc atagaggaat gttgagaacg gtacccaaac tttcgcggag
    51361 gtactcacca tccagatgat ataagaggaa aaccacataa acgtaccaat gaaggcaaaa
    51421 cgtggtccga cactattatt catccaggaa tagataccac cttcttcttt gcgataagca
    51481 gctcccattt cagccatcat taaggcgaat ggaataaaga ataacaatgc agaaaatata
    51541 taaaagggaa tcgcactata acccattaag taataagccg atgggctatt ggcaaatcca
    51601 aatacggaag taaagatcat caatatgagt cctatcagac tcatcttttt tatcgtgtga
    51661 ggcatgaaac catccttcat ttcagcgcgt gtataacacg tgcccactga actgcactgc
    51721 gcaacattga gcaggcagcg taaaaaccaa tacagaattg atacctggat gatatcaaat
    51781 atccagtctt aaattgtggc ctttaacgga aaaatgaaaa catatgctac agaatggcgc
    51841 ttatcacgcc attcttcgct gttaattcag taatttttca gaattatgcc cggtcaacgc
    51901 taaaggcgat gacttcagcc agtgtctccg cgcccagcgc caacatcacc agacgatcaa
    51961 cacctaatgc cacgccggaa cagtcaggca taccgacttt caaggcttca atcagattct
    52021 ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc
    52081 gttgttgctg ctcacgggca tccgtcaatt catggaaacc attcgccagc tcaatacctt
    52141 tataataaac ctcaaagcgt tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg
    52201 atgcctggct ggctggaaag tggtacacaa aggtcggttt ttctttgcca atatttggct
    52261 ctacgccaaa ggtaaacagc aattgtagca gcgtgtcgcg gtcttcttcg gtatcagcaa
    52321 cattgctcaa atccagtttc gctgcgactt cccgcagttg cgttttgtcg gcagagagcg
    52381 ggtcaatttc cagataacgc aagaaagctt gttgataaga aaggctttct gctgccgggc
    52441 agtccagcac ctgttgtaag agatcgtcca cctcgttcat caaccggtac atatcatagt
    52501 gcggtcgata ccactccagc atagtgaact cagggttgtg ataacgcccc atctcttcat
    52561 tacggaagct gcggcacagc tggaataccg gcccacaacc ggcaaccagc aggcgtttca
    52621 tatggtattc cgggctggtc attaaccaga gattcatccc ctgcgaatgc ccggggccaa
    52681 cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac cgtcgcctgg ctcatacaag
    52741 gcgtctccac ctccagcact ccacgatcgg caaagaaacg acggatctcc gccataatcg
    52801 ccgcgcgttt taataagtta ggaatggatg cgctcggctg ccaggatgcc gtttcgctca
    52861 tagttaaatc tccagttttt gacaagggca cgaagtctac tcgcaacgcg acggcgagac
    52921 aaattttacg caggaatcaa acagcggtgg gcagtgacta aaaaaagcac gatctgatgg
    52981 tttagtaatt aaattaatca tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat
    53041 cgatctcgtc aaatttcaga cttatccatc agactatact gttgtaccta taaaggagca
    53101 gtggaatagc gttcgcagac cgtaactttc aggcacttac cctgaagtac ggggctgtgg
    53161 gataaaaaca atctggagga atgtcgtgca aacctttcaa gccgatcttg ccattgtagg
    53221 cgccggtggc gcgggattac gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa
    53281 aatcgcacta atctcaaaag tatacccgat gcgtagccat accgttgctg cagaaggggg
    53341 ctccgccgct gtcgcgcagg atcatgacag cttcgaatat cactttcacg atacagtagc
    53401 gggtggcgac tggttgtgtg agcaggatgt cgtggattat ttcgtccacc actgcccaac
    53461 cgaaatgacc caactggaac tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt
    53521 caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc tggttcgccg ccgataagac
    53581 cggcttccat atgctgcaca cgctgttcca gacctctctg caattcccgc agatccagcg
    53641 ttttgacgaa catttcgtgc tggatattct ggttgatgat ggtcatgttc gcggcgtggt
    53701 agcaatgaac atgatggaag gcacgctggt gcagatccgt gctaacgcgg tcgttatggc
    53761 tactggcggt gcgggtcgcg tttatcgtta caacaccaac ggcggcatcg ttaccggtga
    53821 cggtatgggt atggcgctaa gccacggcgt tccgctgcgt gacatggaat tcgttcagta
    53881 tcacccaacc ggtctgccag gttccggtat cctgatgacc gaaggctgcc gcggtgaagg
    53941 cggtattctg gtcaacaaaa atggctaccg ttatctgcaa gattacggca tgggcccgga
    54001 aactccgctg ggcgagccga aaaacaaata tatggaactg ggtccacgcg acaaagtttc
    54061 tcaggccttc tggcacgaat ggcgtaaagg caacaccatc tccacgccgc gtggcgatgt
    54121 ggtttatctc gacttgcgtc acctcggcga gaaaaaactg catgaacgtc tgccgttcat
    54181 ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt aaagaaccga ttccggtacg
    54241 tccgaccgca cactacacca tgggcggtat cgaaaccgat cagaactgtg aaacccgcat
    54301 taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct
    54361 gggttctaac tccctggcgg aactggtggt cttcggccgt ctggccggtg aacaagcgac
    54421 agagcgtgca gcaactgccg gtaatggcaa cgaagcggca attgaagcgc aggcagctgg
    54481 cgttgaacaa cgtctgaaag atctggttaa ccaggatggc ggcgaaaact gggcgaagat
    54541 ccgcgacgaa atgggcctgg caatggaaga aggttgcggt atctaccgta cgccggaact
    54601 gatgcagaaa accatcgaca agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat
    54661 caccgacacc tccagcgtgt tcaacaccga cctgctctac accattgaac tgggccacgg
    54721 tctgaacgtt gctgaatgta tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc
    54781 acaccagcgt ctggacgaag gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac
    54841 cctcgccttc cgcgatgctg atggcacgac tcgcctggag tacagcgacg tgaagattac
    54901 tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga
    54961 agcagccaat aagaaggaga aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg
    55021 tgcgctataa cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg
    55081 acgcaactac ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc
    55141 tgagttaccg ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca
    55201 acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg
    55261 aagcgttagc taacttcccg attgaacgcg atctggtggt cgatatgacc aacttcatcg
    55321 aaagtctgga agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta
    55381 ctaacatcca gaccccggcg cagatggcaa agtatcacca gttctccggt tgcatcaact
    55441 gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg
    55501 ctgccattac gctggcgcat cgttataacg aagatagccg cgaccacggt aagaaggagc
    55561 gtatggcgca gttgaacagc cagaacggcg tatggagctg tactttcgtg ggctattgct
    55621 ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa
    55681 gttcgaaaga ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact
    55741 aaacgtaaac cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat
    55801 cgcttttaca tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg
    55861 attttcgggc tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt
    55921 ttacaaaacc cggttatcgt gatcattaac ctgatcactc tggcggcagc tctgctgcac
    55981 accaaaacct ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa
    56041 atgggaccag agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta
    56101 atcctgtttg ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg
    56161 ttctgacgaa ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat
    56221 tgcgccggtg atgatcctgc tggtgggtat tctgctgcca ctggggctgt ttccgggtga
    56281 tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct
    56341 gttcctgatg atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca
    56401 cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc tacggtctgg ctgctatcct
    56461 gacagttgtc acgctgattg gtatcgttac aatctaacgt atcgccaatg taaatccggc
    56521 ccgcctatgg cgggccgttt tgtatggaaa ccagacctta tgttcaaaac gacgctctgc
    56581 gccttattaa ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt
    56641 gtgcatcgca caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg
    56701 gtaatttatc agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag
    56761 cagcccgtca cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc
    56821 gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cacaacaaaa
    56881 tactggcctg aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc
    56941 tacactgctg gcggcctgcc attgcaggtg ccggatgagg tgaaatcctc aagcgacttg
    57001 ctgcgcttct atcaaaactg gcagcctgca tgggcgccag gaacacaacg tctgtatgcc
    57061 aactccagta tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag
    57121 caggcgatgc aaacacgtgt cttccagcca ctcaaactca accatacgtg gattaatgta
    57181 ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt
    57241 tcgccagggg cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc
    57301 tgctgggtac gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg
    57361 atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg
    57421 gaaatgctgg actggccggt aaatcctgac agcatcatta acggcagtgg caataaaatt
    57481 gcactggcag cacaccccgt aaaagcgatt acgcccccaa ctcctgcagt acgcgcatca
    57541 tgggtacata aaacaggggc aaccggcgga tttggtagct atgtcgcgtt tattccagaa
    57601 aaagagctgg gtatagtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc
    57661 gccgcctggc agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac
    57721 cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt
    57781 tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgcag
    57841 ttctcctacg ccgccgcgtg gcgtaacagt agtgaataat ttcgacgcca aacgttatct
    57901 cggtacctgg tatgagattg cccgttttga tcaccgtttt gaacgtggac tggaaaaagt
    57961 caccgcaaca tacagcctgc gtgatgacgg cggcctgaat gtcattaata aaggctataa
    58021 ccctgacaga ggaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac
    58081 tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat ggcggttata acgttattgc
    58141 actcgatcgg gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat
    58201 actctcccgc acgccaacca tttctgacga agtgaaacag gagatgctgg cagtcgcgac
    58261 ccgggaaggg tttgatgtca gtaagtttat ttgggtacag cagcccggta gttagtgagt
    58321 gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca ggcgcatcgg
    58381 gttagcggac tcaccgagca gcacaatgcc ggtgatagcc gcgccgactg cgccaatacc
    58441 cgtccacacg gcataagccg tccctactgg taacgatttc atcgcccagg caagtagcgc
    58501 catactgaca atcatcgccg tcacggtaat aacactcggc gtcaaacgac taaagccgtg
    58561 ggtatatttc aggccaacgg cccataccac ttccagcaga ccagcaataa ctaagataat
    58621 ccaggacata tcaggctccg gagaattggg gccgtccccg gtgaaaagag gcgtttgcag
    58681 gtcgtcctgc aaagctgatg gttgaacgga tattttgcca ggtggcaggg gaatttcaac
    58741 tattacctta gtgactatcg cagaaaaaac aaacggcacg acatacctgt cgtaccgtta
    58801 ttgcttattg ctattgctgc gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag
    58861 aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca
    58921 gaacagaaaa gatcgctgca attgtcttct tcaccataac gtcttccttt taggtaatgt
    58981 tatttatgtt tgcctatagc aaattaataa tagacaacat cgccaaaaat gacggaaatc
    59041 tgatgatttt tcggaagaga agaaaaatta gctggcagcg cgagagatgg agttgccgag
    59101 atgtttgatg tcttcgccga aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc
    59161 aagcaaaaca agaacgataa ggcgtttcat catctctgac ctgaataagt gatggtgcag
    59221 cctgcaggcc gcaccacaac cgcattactt cacgcgagag acgtattcac cagagcgggt
    59281 atccactttg atgacttcgc cgatttgtac gaacagcgga accttaacca cagcgccagt
    59341 agacagggta gccggtttgc cgccagtacc tgcggtatca cctttcaggc ccggatcggt
    59401 atcaacgatt tccagttcaa cgaagttcgg cggagtaacg gagatcggct gaccattcca
    59461 cagagttacg atacactctg cctgatccag cagccattta gcgttgtcgc caattgcttt
    59521 tgcatcagca gacagctgct cgaaagtttc gttgttcatg aagtgccaga actcaccgtc
    59581 gttgtacagg taagtcaggt tcatatcgac aacatcagcg ccttcagcgg aatcagtaga
    59641 tttgaaggtt ttttctacgc gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa
    59701 tgcctggcct ttacccggtt ttacgaattc actcgcttca accgcgtaag gttcgccgtc
    59761 taacatgatt ttaagaccag cacgaaaatc gttgctatag tacgttgcca taaggccctc
    59821 tgaaatttgt taattggtag ctaagccaca aaatggcgca tattgtaacc ctaaataccc
    59881 catccagaga agattggtta acgcaacttg ccgatgttgt gaccgatcct gatgaacttc
    59941 tgcgtctttt gaatatagac gcggacgaaa aactgttggc cggacgcagc gccaaaaagc
    60001 tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat ggagaaaggc aatccggacg
    60061 atcctctttt gcgtcaggta cttacctcgc aagatgagtt tgtcgtcgcg tccggattct
    60121 ccaccgaccc gctggaagaa cagcacagcg tagtgcctgg tttgttgcat aaataccaca
    60181 accgggcgct tttgctggtc aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc
    60241 gccacttccc ctatgccgaa aatcagggca acaagcgtaa ctggcagact gctcttgagt
    60301 atgttgctgc gcatccggaa ctggacgaga tgattttctc cggcggcgat ccgctgatgg
    60361 cgaaagatca cgagctggac tggttgctca cacaactgga agccatcccg catatcaaac
    60421 gtctgcggat tcacagccgt ctgccgattg tgatcccggc acgtatcacc gaggcgctgg
    60481 ttgaacgctt tgcccgttct acgctgcaaa tcttgctggt gaatcacatc aatcatgcca
    60541 atgaggtaga tgaaacgttc cgtcaggcga tggctaaatt gcgccgtgtc ggtgtcaccc
    60601 tgcttaacca gagcgttctg ttacgtggtg tgaacgataa cgcacaaacg ctggcaaacc
    60661 tgagtaatgc gttgttcgat gccggcgtaa tgccgtatta cctgcatgtg ctcgataaag
    60721 tacagggcgc ggcgcatttt atggtgagtg atgacgaagc acggcagatt atgcgtgagt
    60781 tgctgacact ggtgtcgggt tatctggtgc cgaaactggc gcgagaaatc ggcggcgaac
    60841 ccagcaaaac gccgctggat ctccagctac gccagcagta actgattaaa cagggaagtt
    60901 aaacattagc ctgatgcgct acgcttatca ggcctacatt gtgcattgaa attgaatgga
    60961 tttgcaatga actgtaggcc ggataaggcg tttacgccgc atccggcaag agcaacaaac
    61021 gcaagtacag caacctgtca ttaaactggg aaatttaatc tttgcacgtt gcataacctt
    61081 taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa taattaacct
    61141 ttcttttctg tttatataga gtgagcacgg
//
LOCUS       DAZFWP020000008        58465 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFWP020000008 DAZFWP020000000
VERSION     DAZFWP020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 58465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.008"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..391
                     /locus_tag="ACHK0Z_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             <1..391
                     /locus_tag="ACHK0Z_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HGU4500539.1"
                     /translation="CIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGL
                     GISVHDISAQKISLLSFQNYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWF
                     FLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            388..879
                     /locus_tag="ACHK0Z_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     CDS             388..879
                     /locus_tag="ACHK0Z_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HGU4500540.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(921..1871)
                     /gene="corA"
                     /locus_tag="ACHK0Z_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     CDS             complement(921..1871)
                     /gene="corA"
                     /locus_tag="ACHK0Z_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU4500541.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2023..2079)
                     /gene="ysgD"
                     /locus_tag="ACHK0Z_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     CDS             complement(2023..2079)
                     /gene="ysgD"
                     /locus_tag="ACHK0Z_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU4500542.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2241..3005
                     /gene="yigE"
                     /locus_tag="ACHK0Z_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     CDS             2241..3005
                     /gene="yigE"
                     /locus_tag="ACHK0Z_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU4500543.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3152..5314)
                     /gene="uvrD"
                     /locus_tag="ACHK0Z_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3152..5314)
                     /gene="uvrD"
                     /locus_tag="ACHK0Z_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU4500544.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5398..6114)
                     /gene="yigB"
                     /locus_tag="ACHK0Z_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     CDS             complement(5398..6114)
                     /gene="yigB"
                     /locus_tag="ACHK0Z_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU4500545.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6114..7010)
                     /gene="xerC"
                     /locus_tag="ACHK0Z_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     CDS             complement(6114..7010)
                     /gene="xerC"
                     /locus_tag="ACHK0Z_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU4500546.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7007..7714)
                     /gene="yigA"
                     /locus_tag="ACHK0Z_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     CDS             complement(7007..7714)
                     /gene="yigA"
                     /locus_tag="ACHK0Z_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGU4500547.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7711..8535)
                     /gene="dapF"
                     /locus_tag="ACHK0Z_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             complement(7711..8535)
                     /gene="dapF"
                     /locus_tag="ACHK0Z_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU4500548.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8572..8775)
                     /gene="yifL"
                     /locus_tag="ACHK0Z_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             complement(8572..8775)
                     /gene="yifL"
                     /locus_tag="ACHK0Z_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4500549.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9125..9679
                     /locus_tag="ACHK0Z_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             9125..9679
                     /locus_tag="ACHK0Z_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HGU4500550.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            10017..11525
                     /locus_tag="ACHK0Z_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             10017..11525
                     /locus_tag="ACHK0Z_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGU4500551.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            11522..12847
                     /locus_tag="ACHK0Z_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             11522..12847
                     /locus_tag="ACHK0Z_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGU4500552.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            12895..13215
                     /gene="cyaY"
                     /locus_tag="ACHK0Z_RS02375"
                     /old_locus_tag="pgaptmp_000475"
     CDS             12895..13215
                     /gene="cyaY"
                     /locus_tag="ACHK0Z_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU4500553.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(13255..15801)
                     /gene="cyaA"
                     /locus_tag="ACHK0Z_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             complement(13255..15801)
                     /gene="cyaA"
                     /locus_tag="ACHK0Z_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU4500554.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            16188..17129
                     /gene="hemC"
                     /locus_tag="ACHK0Z_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             16188..17129
                     /gene="hemC"
                     /locus_tag="ACHK0Z_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU4500555.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            17126..17866
                     /gene="hemD"
                     /locus_tag="ACHK0Z_RS02390"
                     /old_locus_tag="pgaptmp_000478"
     CDS             17126..17866
                     /gene="hemD"
                     /locus_tag="ACHK0Z_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU4500556.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            17888..19099
                     /gene="hemX"
                     /locus_tag="ACHK0Z_RS02395"
                     /old_locus_tag="pgaptmp_000479"
     CDS             17888..19099
                     /gene="hemX"
                     /locus_tag="ACHK0Z_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU4500557.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFIAIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            19102..20298
                     /gene="hemY"
                     /locus_tag="ACHK0Z_RS02400"
                     /old_locus_tag="pgaptmp_000480"
     CDS             19102..20298
                     /gene="hemY"
                     /locus_tag="ACHK0Z_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU4500558.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            20976..21113
                     /locus_tag="ACHK0Z_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /pseudo
     CDS             20976..21113
                     /locus_tag="ACHK0Z_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(21221..22456)
                     /gene="aslB"
                     /locus_tag="ACHK0Z_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             complement(21221..22456)
                     /gene="aslB"
                     /locus_tag="ACHK0Z_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4500559.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCLKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(22603..22679)
                     /locus_tag="ACHK0Z_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     tRNA            complement(22603..22679)
                     /locus_tag="ACHK0Z_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22643..22645),aa:Pro,seq:tgg)
     gene            complement(22722..22808)
                     /locus_tag="ACHK0Z_RS02420"
                     /old_locus_tag="pgaptmp_000484"
     tRNA            complement(22722..22808)
                     /locus_tag="ACHK0Z_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22772..22774),aa:Leu,seq:cag)
     gene            complement(22829..22904)
                     /locus_tag="ACHK0Z_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     tRNA            complement(22829..22904)
                     /locus_tag="ACHK0Z_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22869..22871),aa:His,seq:gtg)
     gene            complement(22963..23039)
                     /locus_tag="ACHK0Z_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     tRNA            complement(22963..23039)
                     /locus_tag="ACHK0Z_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23003..23005),aa:Arg,seq:ccg)
     gene            complement(23142..24527)
                     /gene="yifK"
                     /locus_tag="ACHK0Z_RS02435"
                     /old_locus_tag="pgaptmp_000487"
     CDS             complement(23142..24527)
                     /gene="yifK"
                     /locus_tag="ACHK0Z_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4500560.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(24718..25458)
                     /gene="wecG"
                     /locus_tag="ACHK0Z_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     CDS             complement(24718..25458)
                     /gene="wecG"
                     /locus_tag="ACHK0Z_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU4500561.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(25461..26813)
                     /gene="wzyE"
                     /locus_tag="ACHK0Z_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     CDS             complement(25461..26813)
                     /gene="wzyE"
                     /locus_tag="ACHK0Z_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU4500562.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26810..27889)
                     /gene="rffT"
                     /locus_tag="ACHK0Z_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /gene_synonym="wecF"
     CDS             complement(26810..27889)
                     /gene="rffT"
                     /locus_tag="ACHK0Z_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU4500563.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFAARGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(27886..29136)
                     /gene="wzxE"
                     /locus_tag="ACHK0Z_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             complement(27886..29136)
                     /gene="wzxE"
                     /locus_tag="ACHK0Z_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU4500564.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(29138..30268)
                     /gene="rffA"
                     /locus_tag="ACHK0Z_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(29138..30268)
                     /gene="rffA"
                     /locus_tag="ACHK0Z_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU4500565.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30273..30947)
                     /gene="rffC"
                     /locus_tag="ACHK0Z_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /gene_synonym="wecD"
     CDS             complement(30273..30947)
                     /gene="rffC"
                     /locus_tag="ACHK0Z_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU4500566.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(30925..31806)
                     /gene="rfbA"
                     /locus_tag="ACHK0Z_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     CDS             complement(30925..31806)
                     /gene="rfbA"
                     /locus_tag="ACHK0Z_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4500567.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31825..32892)
                     /gene="rffG"
                     /locus_tag="ACHK0Z_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     CDS             complement(31825..32892)
                     /gene="rffG"
                     /locus_tag="ACHK0Z_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4500568.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(32892..34154)
                     /gene="wecC"
                     /locus_tag="ACHK0Z_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             complement(32892..34154)
                     /gene="wecC"
                     /locus_tag="ACHK0Z_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU4500569.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(34151..35281)
                     /gene="wecB"
                     /locus_tag="ACHK0Z_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             complement(34151..35281)
                     /gene="wecB"
                     /locus_tag="ACHK0Z_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4500570.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(35337..36383)
                     /gene="wzzE"
                     /locus_tag="ACHK0Z_RS02490"
                     /old_locus_tag="pgaptmp_000498"
     CDS             complement(35337..36383)
                     /gene="wzzE"
                     /locus_tag="ACHK0Z_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU4500571.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(36395..37498)
                     /gene="wecA"
                     /locus_tag="ACHK0Z_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             complement(36395..37498)
                     /gene="wecA"
                     /locus_tag="ACHK0Z_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU4500572.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(37738..38997)
                     /gene="rho"
                     /locus_tag="ACHK0Z_RS02500"
                     /old_locus_tag="pgaptmp_000500"
     CDS             complement(37738..38997)
                     /gene="rho"
                     /locus_tag="ACHK0Z_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU4500573.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(39082..39183)
                     /gene="rhoL"
                     /locus_tag="ACHK0Z_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             complement(39082..39183)
                     /gene="rhoL"
                     /locus_tag="ACHK0Z_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU4500574.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(39324..39653)
                     /gene="trxA"
                     /locus_tag="ACHK0Z_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             complement(39324..39653)
                     /gene="trxA"
                     /locus_tag="ACHK0Z_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU4500575.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39784..41049
                     /gene="rhlB"
                     /locus_tag="ACHK0Z_RS02515"
                     /old_locus_tag="pgaptmp_000503"
     CDS             39784..41049
                     /gene="rhlB"
                     /locus_tag="ACHK0Z_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU4500576.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            41183..42667
                     /gene="gppA"
                     /locus_tag="ACHK0Z_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             41183..42667
                     /gene="gppA"
                     /locus_tag="ACHK0Z_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU4500577.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(42714..44735)
                     /gene="rep"
                     /locus_tag="ACHK0Z_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             complement(42714..44735)
                     /gene="rep"
                     /locus_tag="ACHK0Z_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU4500578.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALTVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            44952..45401
                     /locus_tag="ACHK0Z_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             44952..45401
                     /locus_tag="ACHK0Z_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4500579.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            45600..45881
                     /gene="ppiC"
                     /locus_tag="ACHK0Z_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             45600..45881
                     /gene="ppiC"
                     /locus_tag="ACHK0Z_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU4500580.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(45968..47443)
                     /gene="ilvC"
                     /locus_tag="ACHK0Z_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             complement(45968..47443)
                     /gene="ilvC"
                     /locus_tag="ACHK0Z_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU4500581.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            47593..48486
                     /gene="ilvY"
                     /locus_tag="ACHK0Z_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             47593..48486
                     /gene="ilvY"
                     /locus_tag="ACHK0Z_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU4500582.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(48538..50082)
                     /gene="ilvA"
                     /locus_tag="ACHK0Z_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             complement(48538..50082)
                     /gene="ilvA"
                     /locus_tag="ACHK0Z_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU4500583.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(50085..51935)
                     /gene="ilvD"
                     /locus_tag="ACHK0Z_RS02555"
                     /old_locus_tag="pgaptmp_000511"
     CDS             complement(50085..51935)
                     /gene="ilvD"
                     /locus_tag="ACHK0Z_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU4500584.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(52000..52929)
                     /gene="ilvE"
                     /locus_tag="ACHK0Z_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             complement(52000..52929)
                     /gene="ilvE"
                     /locus_tag="ACHK0Z_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU4500585.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIDVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(52949..53212)
                     /gene="ilvM"
                     /locus_tag="ACHK0Z_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             complement(52949..53212)
                     /gene="ilvM"
                     /locus_tag="ACHK0Z_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU4500586.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(53209..54855)
                     /gene="ilvG"
                     /locus_tag="ACHK0Z_RS02570"
                     /old_locus_tag="pgaptmp_000514"
     CDS             complement(53209..54855)
                     /gene="ilvG"
                     /locus_tag="ACHK0Z_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU4500587.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54858..54908)
                     /gene="ilvX"
                     /locus_tag="ACHK0Z_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             complement(54858..54908)
                     /gene="ilvX"
                     /locus_tag="ACHK0Z_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU4500588.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(54995..55093)
                     /gene="ilvL"
                     /locus_tag="ACHK0Z_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             complement(54995..55093)
                     /gene="ilvL"
                     /locus_tag="ACHK0Z_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU4500589.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            55447..56967
                     /gene="yifB"
                     /locus_tag="ACHK0Z_RS02585"
                     /old_locus_tag="pgaptmp_000517"
     CDS             55447..56967
                     /gene="yifB"
                     /locus_tag="ACHK0Z_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU4500590.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(56992..57330)
                     /gene="maoP"
                     /locus_tag="ACHK0Z_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             complement(56992..57330)
                     /gene="maoP"
                     /locus_tag="ACHK0Z_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU4500591.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            57449..58288
                     /gene="hdfR"
                     /locus_tag="ACHK0Z_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             57449..58288
                     /gene="hdfR"
                     /locus_tag="ACHK0Z_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU4500592.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(58384..58459)
                     /locus_tag="ACHK0Z_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     tRNA            complement(58384..58459)
                     /locus_tag="ACHK0Z_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58424..58426),aa:Trp,seq:cca)
BASE COUNT        14444 a        15616 c        14767 g        13638 t
ORIGIN      
        1 atgtatcgaa aatggcatta attcaactcc ctcgacagga taccaacctc ttatcctgca
       61 aagcattaaa accaaattac tttcatcaag actaaataaa tttaatgact tcgaggacag
      121 ttttaatggc ttaggaatca gtgttcatga tatttcagct caaaaaatat ctcttttaag
      181 ttttcaaaac tacgctatag gatggagtgc aacaattcat tttgtagcac aggaccactt
      241 cggtcttgat gtaactgata tcaaaaacaa gacttacagc aaatatagat tttttcgcat
      301 atggttcttt ttacaacggc ataaagactt tgctttcaaa ccttttttca ccaattttaa
      361 cactatcgaa agaatcgaaa attatctatg aaaagcaaac tccagaaaat tatattatgt
      421 ttatttcttc tatgctgcat ctataattta tggacattgc gtcctgtaca gattttgtat
      481 acatactctg atgctggcaa ttcagtcttt ctggtcgttg atcatctgcc atggactgac
      541 agcgataaaa taaattggta tctgaagcat cagaatgaaa ttaaaaatca acatccccta
      601 ccagaaggta gttggcatac ctggtatgtc attgatatcg gtaatggctt cactgactat
      661 aaaaaatata tcgaagggcc gtatgaagat ctctactgtt tcccgacaat taagagcaat
      721 gataattgca tcgtgaaaaa ttacttaatg gttattaatg aataccctta tcgtaacact
      781 catattggta ttaacgattt tactgagtat caattaacac aagaaaataa aatagagcga
      841 gttttcaatc cgcatgactt taaaaaagac aatttttaga aataagccgg ataaaaaaat
      901 agctttatcc ggcactcata ttacaaccag ttcttccgct taaagtacag atacggtgcc
      961 aggcccgcga ggatcataaa gataatcgcg cctgggtagc cgaaactcca cttcaactct
     1021 ggcataaatt caaagttcat cccgtagctg gaagccacca gcgtcggtgg caggaacacc
     1081 acggatacca ccgagaagat tttgatgatg cggttctgct cgatgttgat aaagcccatc
     1141 gccgcctgca tcaggaagtt caccttctgg aacagggatt cgttatgcgg cagcagggat
     1201 tcgatatctc gcaggatttc acgcgcctgc tccagttgcc cacccggtaa acgcgccttg
     1261 cgtaccagga agttgagcgc gcgctgggta tccatcagac acaggcgaac tttccagccg
     1321 atatcttcca gttccgccag cgtggagagc gcctcgtcgt actcatcgcc ctgatgccct
     1381 tccataatca cccggctcaa ctgctccagg tcgctataga tattttcaat ttcatctgcc
     1441 aactgttcga ttttggtttc gaacagatcc agcagcaact cgtaggcgtt accgtctacc
     1501 atcgactggc tacgggcacg catacgatac agacgaaaag cgggcagttc acgctcacgc
     1561 agagtaaaca gacgaccatc acggatggta aatgccacag tggagttacc ggcgtgatct
     1621 tccgcatctt caaagaagaa gaaggagtga atatgcaggc cgtcgtcgtc ttcaaagaaa
     1681 cgtgccgatg cttcgatgtc ttccagttcc gggcgggttg ccaggctctg gccaagttca
     1741 gattgtacgc gcagtcgctc gtcgtcgtcc ggttcgacaa gatcaatcca tactgcattt
     1801 acaaggggtt gtgactcttc gacttccagc cgggtcagtc ggttattttc cagttgaaat
     1861 gcgctcagca tgaccgggac tcccaatgct taaaatatcg gacagttcgg tgggcacaca
     1921 gaaacaaatt gggtttcaga ccattaaaca gcctgactca gcgcgacggg aaaaattgag
     1981 gtcgctgaca accgctaagg ctatcagcaa aaagggatag ccttaggagt tgatcctgga
     2041 tgacaggata gtgagccagt atctactggg tgtgtccaag gcgaatgtcc tcttagagtg
     2101 atcgtgcgcg catgttacgc cagcaaaatt ttgccgtcaa cacgcaacag aacaccacag
     2161 aacaatagtt gaacttcgcg ctttttggta ctcgtcaagg tcgctaacag gataaaaaaa
     2221 tccaacatcc aggacctata atggcgcatc ggctactcat tggtaaagga atgatcacct
     2281 tgaatctcaa acggattttt ctcaccctca ccttacttcc cttgtttgct gttgccgctg
     2341 atgattgcgc actctcagat ccgacgctga ccgtacaggc gtataccgtt aatcctcaaa
     2401 cagagcgggt gaaaatgtac tggcaaaaag ctaatggcga agcgtgggga acgttacatg
     2461 ctctgctggc ggatatgaat agtcagggtc aggtgcagat ggcgatgaac ggcggcatct
     2521 atgatgaaag ctatgcgccg ctcggtttgt acatcgaaaa cggtcagcag aaggtggcgt
     2581 taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg ttttatgtcg
     2641 cgggagataa agtcggcatc gttcgtctgg atgccttcaa aaccagtaaa gagattcagt
     2701 ttgcggtgca gtcagggcca atgttgatgg aaaacggtgt aattaatccg cgtattcatc
     2761 ccaacgtcgc ctcacgcaaa attcgtaacg gtgttgggat taataaacat gggaatgccg
     2821 tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat gccaaagcga
     2881 aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg tatatgaaag
     2941 gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc gagcggaaag
     3001 gttagaatga tagttttgtg tcagatacta aatatgctgc tgatgccgga tgcggcgtaa
     3061 acgccttatc cggcctacat gacgttgcaa tttattgaat ctgaatgatt ttttaggcca
     3121 aataaggtgc gcagcaccgc atccggcaac gttacaccga ctccagccgg gcataagccg
     3181 ccaccagcca tttaataccc tggccctgaa atgccacctg caaacggcta tgctcaccgc
     3241 tgccttccat attgacaatg gtgccttcac caaacttagc gtggcgtacg cgctggccga
     3301 gcttgtagcc gctgtcgttc tcgaccatcg gcgtacccat ccgctgatgg ctgaccgggc
     3361 ggcttaccgt ggcgcgcagg cgcacctctt ccacacactc ttccggcagc tcgccaataa
     3421 agcgcgacgg gcgatggtaa acctctttgc catacagacg gcgagtttcc gcgtaggtca
     3481 gcgtcagttt ctgcatcgcg cgggttacgc caacgtaggc cagacggcgt tcttcttcca
     3541 gacgcccgcc ttcatccagc gacatctggc ttgggaacat accctcttcc ataccgacaa
     3601 taaacacctg cgggaactcc aggcctttcg ccgagtgcag cgtcatcaac tgcactgcat
     3661 cctgccaggt atccgcctgc ccctcgcccg cttccagcgc cgcatgggag aggaatgcct
     3721 gcagcggcat taaatcttca tcttcttcgt tgtagctgaa ctggcgcgtt gccgtcacca
     3781 gttcctctaa gttttcgata cgcgtctggc ctttttcgcc cttctcctgc tcgtacatgg
     3841 tgcgcaggcc ggagtcttta attacccggt cagtctgtac atgcagcggc atatcggcag
     3901 tttcttgcgc taaggcatcg attaattcca taaatcgctg caaggcgctg gcagctcgcc
     3961 cggcgagggc tttttcctgc aacagttcac gacatgcctg ccagagtgtt aactggcgat
     4021 cgcgcgatgt ctgacgtacc acgtccagcg tccggtcacc aataccccgc gttggcgtat
     4081 tcaccacgcg ctcaaacgcc gcgtcgtcgt tacggttggc aatcagacgc agatacgaga
     4141 gcgcgtcttt gatttcctgg cgttcgaaga agcgcatccc gccgtaaata cggtacggca
     4201 tactggcctg caacagtgct tcttccagta cacgcgactg ggcgttactg cggtagagaa
     4261 tggcgcactc ggcaagcgcc ccgccgttgt cctgccaggt tttgatgcgg ttaaccacaa
     4321 aacgcgcttc atcgagttcg ttaaaagcgc aatagaggga aataggctca ccgtccgcgc
     4381 catcggtcca cagtttttta cccagacgcc cgttattgtt ttcaatcagg gcgttagcgg
     4441 cgctcagaat attgctggta gagcggtagt tttgctccag acgaatagtt tcggcaccgg
     4501 ggaaatcatt aaggaaacgc tgaatattct ccacctgcgc cccgcgccag ccgtagattg
     4561 actggtcgtc atcaccgacg atcatcactt tgccggtgtc gcccgccagc aggcggatcc
     4621 acgcgtactg aatgttgttg gtatcctgga attcgtccac caggatattg gtaaaacgtt
     4681 cgcggtagtg ttgtaggata tgcggcttgt taagccacag ttcgtgagcg cgcagcagca
     4741 gctcggcgaa gtccaccagg cccgcgcggt cacacgcttc ctgatacgcc tgatacacct
     4801 tctgccaggt ctgctccacc ggattaccgt agctttgaat atgatgcgga cgcaggcctt
     4861 catctttctg gctgttgatg taccacattg cctggcgcgg cggccactgc ttctcgtcga
     4921 ggttcatggc tttgatcaga cgcttaagca ggcgcagctg gtcctcgcta tcgaggatct
     4981 ggaaatcctg cggcagattg gcgtccatat ggtgcgcacg cagcaggcgg tgcgccagcc
     5041 cgtggaaggt gccgacccac atgccgccct ggctggtgcc catcagttgc ccgatacgat
     5101 gacgcatctc cgccgccgct ttgttggtaa acgtcaccgc cataatcgag tatggcgagc
     5161 agttttccac gctcatcagc caggcgatac gatgcaccag tacgcgcgtc ttaccactgc
     5221 ccgcgcccgc cagcaccaga aggttgctgc gtggcgcggc caccgcttcg cgctgtttgt
     5281 cattaaggct gtcgagcagg taagaaacgt ccattggcac cgccgcgtaa aaataggttg
     5341 ggcggagaag cgcaacgccc tccgccaaaa agctgggtat atatacagat ttgctgatta
     5401 tatcagcgag gtcagagatg ccaaccggga aatttccaga tgcggcagta aacggctgtc
     5461 ccaggtttgc atcagatcgc cattttccgg tctgatccaa caagcctgca ttccgctgcg
     5521 aattgcccca cccacgtcag tggtgagatc gtccccaaca tgtaagatct cgccgatcgg
     5581 cacgttgagt ttttccgcag ccaaaaagta catatcgctg aacggttttg agcgcccgtg
     5641 cgggccagcg cgcagcacaa actcaaaata atcccccaaa ccaaacagtt ccggctgggc
     5701 gttaccgttg gtgatcgcca ccagcggcca tttcttcgcc agctgtttta aggtgtcgtg
     5761 agtttgctgc gggacgtcga ttcggctgcg ccatttggca aagttaatca ttgctgcgtg
     5821 tgcgcctgca ctggcttctt cggcactcag cccggcgtcg agcatcgctt gttcaatcga
     5881 acgaaaacgc caacgcgtca cgtcgtgata aatctcgggt tccgcttccc gtaccgcctg
     5941 gcgcaggcgt tgcagatctt cattctggaa gctgcgcagc gccggatgat aattttgcac
     6001 aaaggtaagc gcctctcgct cggtgcgcaa aatcaccgga cggttatcgt aaagggtatc
     6061 atccaggtca aaggtgagcg ccgagatgcg ccccaaaggc cggtaaaaac gcattatttc
     6121 ccccgtttgg cgcgtggatg cgccgcatcg tacaccgagg caaggtgttg aaaatcaaga
     6181 tgagtataga tttgcgtggt ggagaggttg gcatgaccca gcagctcctg cacaccacga
     6241 agatcgccgc tcgactccag catatgcgta gcgaacgagt gacgtaattt atgtgggtga
     6301 acgtgattat tcagcccttg ttttatgccc cattcggcaa agcgtttctg cacattacgc
     6361 gcggagatac gcttgcccag tttcgacaga aaaagcgcgt cgtcttcgct accaaacagg
     6421 tcgcgcaaat caagccagtg ctcaatccac gccacagcgt tgcgaccaat cggcaggcgg
     6481 cgctctttgc tgcctttccc cattacccac acttcaccag actccaggtc gaggtgtttg
     6541 atatccagcc ccaccagctc ggaaagacgc agacccgcgc cgtacatcac ttccagcatt
     6601 gcacggtcgc gtacagcgag gggatcgttg atatcaatat ccagcagccg attcatatcg
     6661 tcgacgtcga tgtttttcgg cagatgacgc ggcgctttcg gtgccgaaac tcctttcgcc
     6721 gggttagctt tgagttcgtt ctggctgacc agccagtcaa aaaagctacg tagcgcagaa
     6781 agccgtaacg ccagacttgc tgctcccagc cctttacggc gactgcgtac agcaaaattg
     6841 cgcaccatcg tcacatcaca ttgctgccag ctttgcaggc cgttttcgct ggcaaaattg
     6901 atgatcgcct caagctgacg ctggtagtta agcagggtta tcgggctaag ctggcgctcc
     6961 acgctcagat aacgtaggta gcgttctaca tcggtgtgta aatcggtcat acgcgttcaa
     7021 tccaacgctc cagaagctcc ggcaacatca gcgcaatttc atgaagtaac tgcgttcctt
     7081 gcccttgttg atagtgactg gcatcgcgac tggtaaacag cacaacaccc aaatcagcat
     7141 cgcttcccag catcgacatc gccaccgatc ccaccgcttt cgcttccggt agcaccacca
     7201 gcaactctgg tccgttaagc ggcccaagat agtgctgttc ctgccccaaa cgctgaatac
     7261 gcagcggttc gaaagactga cggcttaatg ccagatgagt gtggttcgac ggcgcaccta
     7321 agcgccagcg atccggaaac aggcgcagac tcgcacctgc caggccgaga tcgcgcgccc
     7381 agcggtgaaa gcgcatcagc atatcgtcga gactgctggc ggcggtgaga ctgcgctgca
     7441 ggtagagtag gcgataaaac aggccttcgt tggcgatagc ctgttccatc aacagcgcca
     7501 tgttctcttc cagaacatga atatgattac gtgcgcgggc catgtgccac tcgaccaacg
     7561 aaacggtgcc gcgtaccgga tgcggcacac gtatcgcttc tactgcgcgc gcattacgga
     7621 taaaaaactc aggattttta atcagataat cgacaaccgc ccggtcatca agctccgtga
     7681 gtgtttcctg cagttcttcc cctggttgct tcatagatga ataaatccgt cgtagacatg
     7741 taccgccggg ccagtcatat ataacgggtg acccggacct ttccaggcga tatcaagacg
     7801 accgccgggg agttccacgc gtacttcttc ggccagcaaa ccttgctgaa tccctaccgc
     7861 caccgccgca catgcgccgc tgccgcaggc ctgggtttct cctgccccac gctcataaac
     7921 gcgtaaacga atatgctcgc gcttaaccac ttgcataaaa ccgatattgg ctcgctccgg
     7981 aaaacgctcg tggctttcca gaacaggacc aagcgtttct accgccgcgg tatcgacatc
     8041 atcgacctga atcacgcaat gcggatttcc catcgacacc acgccgcata agattgtctg
     8101 ctcggcggcg cgcataatat aggtcttttc cgctttgtta gcgcgaaacg gcacggcgga
     8161 aggttcgaag ttgggttcgc ccatatttac gcggaccaga tcatcatcgg tgacggtcag
     8221 aaccatccgc ccgttggcgg tgctgacgcg gatatcgcgc ttattggtca gccctttcag
     8281 acgcacaaaa cgggcaaagc agcgcgcacc attgccgcac tgcgccactt cactgccatc
     8341 agcattgaaa atgcgatagt gaaaatccag ttcaggatca tacggcggct caaccaccag
     8401 cagttggtca aaccctactc ccaggtgccg atcagccagg cgacgaatca gctccggtga
     8461 aaaaaagaca ttctgcgtta ccgcgtcgac gaccataaaa tcgttgccaa ggccatgcat
     8521 tttcgagaac tgcatcattt actccaatca cgcgggtaca gaaactgact tttagtaatt
     8581 cacctgggat ggaccatcgc cagtggcgcg atcgttttta tccggcaccg tggattgcgt
     8641 ttgcgtctct accggtttgg tcggcggcgg tgcgttttta tctgcaggcg ggaaatagag
     8701 cggacctttc agaccgcagc ccgtcaggct gaagagagta attaatacag tgagtgcctt
     8761 aaacacgttt ttcattatgg attgcctgta aagttcatcg ctttctgctt tctatcatcg
     8821 caggagaagg cgtaaaagca agcgtttagc gactctttat gaagtctgaa aagtgaggaa
     8881 atgctgcgag tataacgctt acacgtgggg atttatgttt tgctggtagt ttcgtgatcg
     8941 gcacctcgcg ccaggatggc tttatgcact atcgcaatgt ggtttgtttg ttgtcttgtt
     9001 cattgttctt atcgtcggca tggggctttt aatagagtgg cgcaccatgc aatttcgttg
     9061 tctgacctta gtcgccagtg acatacaact ttgccaacct ggtgataaga gggaataaaa
     9121 tctgatgggg ctatttaccc aactggaaaa atttgaccaa aagcttacgc gcggttatgc
     9181 ccggtggggg cgctgggtat ggcggacgtt aattgtcgtt ccggtactgg tcgtgttgtg
     9241 gaatatagga caggcagtat ggggaggacc aagaggtgga gtgattctgg aaatccacag
     9301 tgaaattgac cgcccgatac tgggattcag tgttaacggc gtggcgggcg cgaatgcttt
     9361 tgcaaatggt ggcggttcaa caacctgttg tggtgatgtt agtggggata cggcagaggt
     9421 tatctggaca ttaagtacta ctcgtacaca gtataatgct ggaatgcgtc tggagaaacg
     9481 gaatatgacg ttacctctcc ccaaaagaga atggggagaa gattttctgc atgtgcattt
     9541 tatgcctggc gataaagtgc tgctggggtg gagtaaagat tctttttctc cgtatgaaga
     9601 tcttcacaac ggggggtaca agacccgcgt tcggcaggat gtgaaagata aactttatgg
     9661 tacagggaaa atgaattgat agatttctat aaaaatcacg ttcggcagaa agcgtaataa
     9721 tgtggggaat gttagatacc ggtggtggga cctaagtaca acacgcgaaa gacaataaag
     9781 taggtaacgg tgggcagagt cgcatgttga cacccttccc ggcgtcaatc cggcacattt
     9841 taacgtgatc agtcacacca tggcgcagta gctcgtattt atatcgtgaa tgaaaacctc
     9901 aagtttaccc cagcaactat cagaaaatag cgatggcctt tatagattct gaaaagtgac
     9961 gatttgccgc aaaagttacg cttacgtgcg aggaattatg ttctgctggt agtttcgtga
    10021 tcggcacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt ttgttgtctt
    10081 gttcattgtt tttatcgtcg gcatggggat gtcggcttga tccgcctgaa cataatattt
    10141 accagaaaca gggaaaaggc gtggtatacc tgcgccctta taaagaaaca aatctttctc
    10201 ttcctgacgt gaattacaaa agtttgcgcc gtttacccaa tttattaata gacccgacaa
    10261 cactagacaa atgggataaa gtacctcctc tgactgatct gacaaccgac tacctctatg
    10321 aaggcgcaca ggcctggtat cctcattact cctggcatag cgatggtcgc tatatcctct
    10381 acgcgggtga agttgtgcaa aacccgccag acaaacctcc cgtcgatgtc gcctcgttca
    10441 aggcgtgggg tgattttgcc gcagataaac acagtctcta tttcgaaggc aagcgcaccg
    10501 ataacaatgg cgggggaaat cgcttagata tcaaaacact gcatcaggtg gaattccggc
    10561 ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc ttgtatatca
    10621 atgggcatcg cctcgcagat cccgatagtt tccgtgtgct ggcacaaaag tcatgggatc
    10681 agcgaggaaa gttttctacc gcgtttaatc cctgtgtggc tatgccattt ggcccctggg
    10741 ataccctggc tcgtacacgg accaaaatcc tgatcaacgg cgaacagctt gatgccgacc
    10801 cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg cgtgataaaa
    10861 acgggctgca gcgtaaagtc ctcgacaagg aaaatctgga gtgggatgaa gatttaacaa
    10921 agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa ggacctgctt
    10981 gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc agtgatgctg
    11041 tcgcccagta tcaggactcg ctttatacca tcatcgaaac agagtctggt gaccgcaagc
    11101 tggaaatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc aacgccggga
    11161 aacgccacgg ctatttactt acgcgtgccg aagggtggcc ataccattcc ggtttacatg
    11221 tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat gaacgcgaag
    11281 cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac cgctacattt
    11341 acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa caagttcgct
    11401 taatctggaa ggaacaacat tcgggatatg gctatggcgt aaattacaaa acgggatatc
    11461 tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat gaggcgacaa
    11521 aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat ttctgacgcc
    11581 tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt cttacggtaa
    11641 aggttattgg ggggataaga aaatcattgc ccaatttccg aagatgaaca gagccgattt
    11701 gcgccttgtg acaaatatat ccggggagcg ggagttggtg gaaggctata tacccaccga
    11761 tgaaataagc ataaaaaatg aggaatacca ctgggtgaca gacggccgcg ttattctctg
    11821 gcgtggcaaa atcgttagca atccaccggg aacacccact gtcgatattg ccagctttca
    11881 ggctatgggc cgcttcgcgg tcgataaata tagcctctat tttgacggac agcgcaccga
    11941 aagtaatagc ggcgcgtcac gtgttgatct ggcgacacta aaagctatcg aaggtaactc
    12001 taccacgctg gtggatagta aaaatctcta tttgtccggt cgacgtcagg ggagcagcag
    12061 taatgttact gtattagaaa aaagatggtg gggtattaat ccacgtctta tgagtgtaaa
    12121 cagaaatttg tattccaatg atctgcttat ccgcagtggg cagaatattt atttaaatgg
    12181 cgttcacctt acggcgaatg cagactcatt tgagataatt cgctggatac ctcattcact
    12241 gctggttttt cgcgacaata agggtatgca tcgttatccc tttgggcaat tgtctggcaa
    12301 agcgataccc gtagatgatg acgtctcttt tgaagtaggg gaaagtcgcg ttcgctggcg
    12361 taaacagctc acgtccgacc gtcagtggag caagtggata gacctaccag atattgaacc
    12421 tgaacaattc catctgatta ctggcaatat tgcgcaatat aaagatcggt tgtatgtaac
    12481 aaaattatcg acatttggtg aagaccagct tgagataatc ccgctggata cgccagacct
    12541 ggtcattgat cgctcattta atagcggcaa acagcatgct tattttatcc gccaattacg
    12601 gtcaaagagc gtgcaaatta ttccagttaa cggtccgcta actaaaaacg atcgcttcgc
    12661 ttatgacgat cgcaatgttt atacatggac cgatacagag gtaaggatta cgccctcccc
    12721 ctgcccggcg aaaacacacg tcagagaaga gaatgtgcgt gaacttcaca acagagacat
    12781 tattattccg ttgacggatg actcatgccg gaacgcagca acagacgggc aaactttgaa
    12841 gccctgaccc cataccgcta tactgccgcc atcacttaaa gacaggatac aacaatgaac
    12901 gacagtgaat ttcatcgcct ggctgatcaa ctctggctga ccattgaaga acgcctggac
    12961 gactgggatg gcgacagcga tatcgactgc gaaatcaacg gcggcgtact gaccattacc
    13021 tttgaaaacg gcagcaaaat cattattaac cgccaggagc cgctgcacca ggtgtggctg
    13081 gcaaccaaac agggcggcta ccattttgac ctaaaaggcg atgaatggat ttgcgatcgc
    13141 agcggcgaaa ccttctggga tttgctggaa caggcggcga cacagcaagc gggtgaaaca
    13201 gtcagtttcc gctaagattg catgccggat aagcctcgct ttccggcacg ttcatcacga
    13261 aaaatattgc tgtaatagcg gcgtatcgtg atcctgattg gcaggcggca tgttaccgat
    13321 agattttgtg cggaacggaa tcacctgttc acgaccatca accttcacaa tctgatagaa
    13381 ctgcggcagg ttgaagttga tgaagcttga gccgtaggtg aagcggtcgt gcgatgacga
    13441 gtagaagcga ctgacgtcac gcaccagttc ctctttgctg ccttcgcagt ggtgatacac
    13501 ctcaacccgg ttgctttcgt cgagaatgta gatattaaag ccattctcgt cttgcgtttc
    13561 ttcgaagaag aactggatga tcccttcact agcaaagccg tccaccaccg ccggtaattt
    13621 gacgtgattg gtttcaacct gcactgacaa gccgtgcagt ttgttatgcg aaatagcgcc
    13681 ataaaactcg atggcgtttt ccagtttttg taccgataca ttcaggcgtt cgaagaacaa
    13741 cccccaggtt tgaccagaaa cgcgcagcgc cttgaaacgc ccggtttcct ggcgggtgct
    13801 ggaaaggcgc agttcaatac actcagaaac cagttgctgc acgcgagtac ggattaaccc
    13861 gcgcagatgc tggctataac agaagacttc cacgctatct ggcggtgcgg cgtcctgatg
    13921 cattttgccg agaatagttt tcagggcttc gatcatcgat tgctcgccgt tgaagtgcag
    13981 cgtacgcact tcgttccacg agttgcggta cagcaggtca acgctaccta ccaggcaatt
    14041 ttgattctcg ccaaagctga agacatccag cttacggaaa tcaaaatgca ccacctgatt
    14101 gcggaacgcc gctgtcgggt catattccag gttaacgata atcgccagat ggcggatctc
    14161 acacgggctg tagagcgcct tcggtgtcgg tgcaggtaag cgcagcggga aatggtgcga
    14221 cacgtcggcg accatctcct gcaacttagg caaatcgaca atgccgttac ctttaatata
    14281 caaacgggtg cgcgaggtca gcaggccgtt aaaccatgcc cacgccacca gtttgttcag
    14341 gtaacggtta tattccagcg gctgatggct gatgatcgac tcaatatttg gcgcgcggtt
    14401 atacagatac caacctgaac ggttagcacg gcccggcggc acataaataa aggtcagatt
    14461 cggttccgag agatcgggtg aaatctgcgg gtttaccagc gtcactttac ctggtaatgc
    14521 ttcaaacgcg gcatacagct tacgcgtcag cacgccgata tcctgcggac tggcggagac
    14581 gctaaggtta ttgcgacgcg caaagcggat cagattacgg tagctctgca tcatcgcgtc
    14641 gagcaactcg ttgtgcgcct cacgcacctg atcaatcttc cagttagcgc ggttatcgag
    14701 cattgccaga cgagcttcgt cccaacccca ctcgctcact aactggctca acactgcgcg
    14761 acgccagcct acgcaggcgc gttcacggct gagcttttcg cacactttta aatagaagca
    14821 gcgacgtact aaatccagac gggtaaaatc ttcaatcgcc gtcaggtatt cagtaacacg
    14881 ctccagcatc atgcagtatg gatcgagacc aaacgataca atctcgccgt cgtgcaaacg
    14941 ctgtttgata tctttcgcca gcagacgtgg gttcgggtat tcccaggaat aggcttccag
    15001 cagcagtgtt ttcagtaccg ctttgtatgg ggaatcgata ctcttgtaga gctgccaaag
    15061 gctggcacca aagtactctt cagcagaaag cgagcttaag ccaccgagat ccagccattc
    15121 atttggcgtc agcacgccct gcgcgtaaag cgtcatcaca tagtcgtcgt aatgctcttc
    15181 ttcgtcgcac ggcaccatat tccacagaat acgcttaccg gcgagacgca cggcggtacg
    15241 ataaaattcg tcaagcagca gtatatgctg ggtggagcca caatcttcgc cccccaggct
    15301 gccgctttca ttatgacgga agcggttttc atcaatcagg aagaagctga cttccacacc
    15361 cagcgaggcg gcccagcttt ccagcagact acatttacgt tgtagtaact ggcgctcttc
    15421 gctatcgagc caggattgat gacagaccca gatatccagg tcagaggaac aactttgccc
    15481 tacggacgag gtgctgccca tggtgtatac accagtaatt ggaagctcac ctttcggtgg
    15541 gtcctgtact gacattccac gatacagttc aagctcgttc aggtagtggc gttgagtttc
    15601 atcaggcgtg taaaggcaaa tgcctttggg aacgttacca tcaaggtaac ccggcattag
    15661 cggatggtga tagtgcaaca atgtcggcag tagactgtag acctgttgga atgcaggccc
    15721 catagcagca agcgcgcgat ccacacgcaa ttgatttatg gcatccagtc tctgtttcag
    15781 agtctcaata tagaggtaca agacgtatcg cctgatttgc tacccgtcat gactgtgatt
    15841 ccgccaacat caacggtaac acgcggcatt cgggatattt cgtatgtcaa aggtaaccgt
    15901 taccactttt cgcgcctggt ttttttagtt tcacgacgaa aaaatggtct aaaacgtgat
    15961 caatttaaca ccttgctgat tgaccgtaaa gaaagatgcg ctacatacaa gtgtagcacc
    16021 gtttattctc tgtaaattcc ttattacgac ggcgtgaaac gcctgtcagg atccactgcc
    16081 agacctcatt ttacggtttg cgcaggcgtc tacgtttcac cacaacactg acatcactct
    16141 ggcaaggatg ttaggatgga ccacggatga taatgacggt aacaagcatg ttagacaatg
    16201 ttttaagaat tgccacacgc caaagcccac ttgcactctg gcaggcacac tatgtcaaag
    16261 acaagttgat ggcgagccat ccgggcctgg tggttgaact ggtaccgatg gtgacgcgcg
    16321 gcgatgtgat tcttgatacg ccgctggcga aagtaggcgg aaaaggctta tttgtaaaag
    16381 agctggaagt cgcgctcctg gagaatcgcg ccgatatcgc cgtacattca atgaaagatg
    16441 tgccggttga attcccgcaa ggtctggggt tggtcactat ttgtgagcgt gaagatcctc
    16501 gcgatgcctt tgtgtccaat aattatgaca gtctggatgc gttaccggca ggcagtatcg
    16561 tcgggacgtc cagtttacgt cgccagtgcc aactggctga acgccgcccg gatctgatta
    16621 tccgctccct gcgcggcaac gtcggcactc gcctgagcaa actagataac ggcgaatacg
    16681 atgccatcat tcttgccgta gccggactaa aacgtttagg tctggagtcc cgcattcgcg
    16741 ccgcgttacc acccgagatt tctcttccgg cggtaggaca aggtgcagtc ggtattgaat
    16801 gccgccttga tgatacgcgc actcgcgagc tgcttgccgc gctgaatcac cacgaaactg
    16861 cactgcgcgt taccgcagaa cgcgccatga atacccgtct cgaaggtgga tgtcaggtgc
    16921 caattggtag ctacgccgag cttattgatg gtgaaatctg gctgcgtgcg ctggtcggcg
    16981 cgccggacgg ttcgcagatt attcgcggtg aacgccgcgg tgcgccgcaa aatgccgaac
    17041 aaatggggat ttcgctggca gaagagctac tgaataacgg cgcgcgcgag atcctcgctg
    17101 aagtctataa cggagacgcc ccggcatgag tatcctggtc acccgcccgt ctcccgctgg
    17161 agaagagtta gtgagccgtc tgcgcacact ggggcaggtg gcctggcatt ttccgctgat
    17221 tgagttttct ccgggtcgac aattaccgca acttgctgat caactggcgg cgctggggga
    17281 gagcgatctg ttgtttgccc tctcgcaaca cgcggttgct tttgcccaat cacagctgca
    17341 tcagcaagat cgtaaatggc cccgactacc tgattatttc gccattggac gcaccaccgc
    17401 actggcgcta cataccgtaa gcggacagaa gattctctac ccgcaggatc gggaaatcag
    17461 cgaagtcttg ctacaattac ctgaattaca aaatattgcg ggcaaacgtg cgctgatatt
    17521 acgtggcaat ggcggtcgtg agctaattgg ggataccctg acagcgcgcg gtgctgaggt
    17581 cactttttgt gaatgttatc aacgatgcgc aatccattac gatggtgcag aagaagcgat
    17641 gcgctggcaa tcccgcgagg tgacgatggt cgttgttacc agcggtgaaa tgttgcagca
    17701 actctggtcg ctgatcccac aatggtatcg tgagcactgg ttactacact gtcgactatt
    17761 ggtcgtcagt gagcgtttgg cgaaactcgc ccgggaactg ggctggcaag acattaaggt
    17821 cgccgataac gctgacaacg atgcgctttt acgggcatta caataactct cataacagga
    17881 agccataatg acggaacaag aaaaaacctc cgccgtggtt gaagagacca gggaggccgt
    17941 ggacaccacg tcacaacctg tcgcaacaga aaaaaagagt aagaacaata ctgcattgat
    18001 tctcagcgcg gtggctatcg ctattgctct ggcggcgggt gtcggtttgt atggctgggg
    18061 taaacaacag gccgtcaatc agaccgccac cagcgatgcc ctggctaacc aactgacggc
    18121 attgcaaaaa gcccaggaga gccaaaaagc cgagctggaa ggcattatta agcaacaagc
    18181 tgcacagctt aagcaggcga atcgtcagca agaaacgctg gcaaaacagt tggatgaagt
    18241 ccaacaaaag gtcgccacca tttccggcag cgatgctaaa acctggctgc tggctcaggc
    18301 cgattttctg gtgaaactcg ccggacggaa gctgtggagc gatcaggacg tcacgaccgc
    18361 tgcagcgttg ctgaaaagtg cagatgccag cctggcggat atgaatgacc cgagtctgat
    18421 taccgttcgt cgggcaatta ccgatgatat cgccagcctt tctgcagttt cgcaggtgga
    18481 ttatgacggc atcatcctta agcttaatca gctttcaaat caggtagata acctgcgtct
    18541 ggccgataat gacagcgatg gttcgccgat ggattccgac ggtgaagagc tttccagttc
    18601 catcagcgaa tggcgtatca atctgcaaaa aagctggcag aactttatgg acaacttcat
    18661 tgcgattcgc cgtcgtgatg acaccgccgt accgctgtta gcgccaaatc aggatatcta
    18721 tctgcgcgaa aatattcgct ctcgcctgct ggtcgcagca caagctgtac cgcgtcacca
    18781 ggaagagact tatcgccagg cgctggagaa cgtctccacc tgggtacgag cttactacga
    18841 tactgatgat gccaccacca aagcgttcct cgacgaggtg gaccagttaa gccagcaaaa
    18901 tatctcgatg gatcttccgg aaaccctgca aagccaggcg atgctggaaa aactgatgca
    18961 gacccgcgtg cgtaacctgc tggcacaacc ggcagcgggg acaacggaag ctaaacctgc
    19021 acctgcacct gcacctgcac ctgcacctgc acctgcaccg caaggtgata ctccggcagc
    19081 cgcgccgcaa ggagaataac catgctaaaa gtgttattac tctttgtttt gctgattgcg
    19141 gggatagtgg ttggcccgat gattgccggt catcagggtt acgtgctgat ccagactgac
    19201 aactacaata tcgaaaccag cgtcacgggc ctggcgatta ttttgattct ggcgatggta
    19261 gtgctgtttg ccattgagtg gctactgcgg cggatcttcc gcacaggcgc acacacccgt
    19321 gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc agaccgaaca ggcgctgctg
    19381 aaactggcgg aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac
    19441 gcggaacaac cggtggtgaa ctatctactg gctgccgaag ccgcgcaaca acgtggtgat
    19501 gaagcacgcg ccaaccaaca tctggaacgc gcagcagagc tggccggcaa tgacaccatt
    19561 ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc
    19621 cacggcgtgg ataagctgct ggaagttacg ccacgccatc cggaagtatt acgtctggcg
    19681 gaacaggcgt atatccgcac aggtgcatgg agttctctgc tggatatcat cccatcaatg
    19741 gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt
    19801 ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag gtttgcgtaa ctggtggaaa
    19861 aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg tggcaatggc ggaacatctt
    19921 attgaatgtg acgatcatga tactgcccag caaattatca tcgatggcct gaaacgccag
    19981 tacgacgatc gcctactgct gccgattcct cgactgaaaa ccaacaatcc ggaacagctg
    20041 gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca
    20101 ctgggccagt cactgatgaa gcacggagaa tggcaggaag catcgctcgc cttccgcgcg
    20161 gcgctgaaac aacgtccgga cgcctacgat tacgcatggc ttgccgacgc gctggacaga
    20221 ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg gtttgatgtt aacgttgcag
    20281 aacaacccac cacagtagtt ccttctcacc cggaggcaag cacctccggg acctttctga
    20341 tgcataaaaa aacgcctgct cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa
    20401 caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg atgaggcata agcgacatct
    20461 gtcagtggac gataagcacc gtaaacggct ctgcgtcatt ccggagttta tgaggcacta
    20521 aggcgaacat aagagatgga atgagcatct acccgtttat tatgccacag agaatcggga
    20581 aataacatcc cttaacactt gttatgagat aattctgtaa tcctctttgc ttcctgagta
    20641 ataacttcct gagtgaatat ttaacctgag cttgatccta cacatactta ttatgaatga
    20701 taaaattcat tcaattaata acacatatat taattgccgt taaaactaaa aaagcatcaa
    20761 taatcaacgt gatataataa acctgcctta catatcaact gcgccagagg taggattgaa
    20821 aacgctctcc tgattttcca attcattttc tggataaata aataatttat ttttatcact
    20881 attatttatg taatcatcct gtcagggaga gggatctcaa ttatcaatgc ttaattacgt
    20941 catcattttg atgacatgaa accggagcat tttgcatgga attttcgttt tcacccaaac
    21001 ttcttgttgt tgctgtcgcc gccgctcttc ctctcatggc cagcgcagca gataccccgt
    21061 caactgccac cgcacgcaaa ggctttgccg gatacgatca cccaaaccag tatccacaga
    21121 acgctgcaat ttattgaatt tgcacgatca tgtaggccgg ataaggcgtt tacgccgcat
    21181 ccggcaatca accgcaggcg gccacaaaat ggccgctttt ttacttattc accaccagca
    21241 aatgtgcctg cataatgtca ctggccgggc gaccgtgcgc cagcaaatca gccattgctt
    21301 taagatatgg cggtagatgg cggaaataac gctgataccc ggcacacaaa taattcagcc
    21361 ccggtttgcc gctggcatcg agcatgaagc ggtgtttcag gcagcctccc cagcacgctt
    21421 ttaacacgtt acaactgcga cactgcgccg gtaattgctt aaatttatct tcaccaaaca
    21481 cctgctgttg cggggaatcg atcatttctg caattgtttg ctgatgcata ttccccagcc
    21541 gatattgcgg ataaacatag tgatcgcagg cgtaaacgtc gccgttgtgc tcaacaatca
    21601 ccgagcgccc acaggttggc tgatgatggc aaaccgcacc cggcgcaccg acaaaattgg
    21661 caaacgccca ttcgatattc atcacgaaaa tcttgccgac gtcgcgtttg atccagtggt
    21721 cgaatatcgc caccagaaat tcaccaaatt catcggggcg caccgaccat tccgtcagtt
    21781 ccccctgaat atcaccaggc gcatgtagtt tcagtccatc gctggcagct gtttcatcag
    21841 ccaggcgctc gaccaccgga ataaactgga tgaattcgac tcccgcatcg cacaaaaaat
    21901 cataaacctg caacggttgc tgcgcgctgg tgcggttgac gcagaccagc acgttatagt
    21961 cgacatgatg tttttgcagc agcgtcaggg cacgcatcac cagcttatgc gttggtctgc
    22021 cacctttggt cacgcgatat tgattgtgga tctcagccgg accgtccagc gataacccaa
    22081 caagaaaatg attttctgcc agaaatgcac accatttatc atcgagcagc acgccgttag
    22141 tctggaagct gttacttatc ttcctgccag caccgtattt cgcctgtaac tttacggcac
    22201 ggcggtaaaa atccagcccg agtaacgttg gttcgccgcc ctgccaggta aaagccactt
    22261 cgttttgcgg ttcgctggca gcgatatagt gacggacata cgcttccagc gtgtcatcgt
    22321 ccatatgcgt gactggcttt tcgcggtaaa gggattgttt ttcgagataa aaacagtagt
    22381 cacagttcag attacaatcg gaaccactcg gtttcgccat cacatgaaaa gcacgcgttg
    22441 gaacctgttg cagcatggtc actccttgcc tgaagtcatc cttcaatacg ttaagggcga
    22501 aatcataccg tggggcaatg agttattacg ggcgtttatt gcgaagggag ggtgacgaaa
    22561 tgcacagaaa acaaaaaacc ccgccgaagc ggggttcaaa attggtcggc gagagaggat
    22621 tcgaacctcc gacccactgg tcccaaacca gttgcgctac caagctgcgc tactcgccga
    22681 aatactgctt tttgaatttt tagttcaatt ctttaaagtc gtggtgcgag gggggggact
    22741 tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc
    22801 accttcgcat tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca
    22861 actggaatca caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt
    22921 acgcggtatt actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat
    22981 tcgaacctga gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct
    23041 tagcgccgtt gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta
    23101 aataaattgc gcgtttggtt acggtttgtg cgttttgctg attattcctc cagtttatgc
    23161 gctttcccgt ggcgattaag gccaaaaact ttataaatcg ccgtcaccgc cagcataaag
    23221 atgatgccaa caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg
    23281 caaatcagga atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga
    23341 tggctggcaa tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca
    23401 aaccatggca ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc
    23461 ggattgggaa tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc
    23521 acacccgcaa cgggtacgcc gtgacgggaa actttcgcca tcgccgccgg taactgacgg
    23581 tttttcgcca gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag
    23641 agcgcagccg tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg
    23701 gcaaaagtca gtacgaacgg gctgccgttg ctgcctattt cattccacgg gaagatggtg
    23761 acgataacga aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac cttgcctacg
    23821 gcactgcgca gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc
    23881 acgccctggt aggacgccac cacaatacac agcgcggtca ggaacccttt ccagccaccc
    23941 gcaaagaaac cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca
    24001 aagccaaaga aaatcacgcc cagaccaatg acaatcatca cgataatcgt ggtgactttg
    24061 atcatcgcga accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac
    24121 gccaccagcg ccactgcgat caatgcgggt atccactgcg ccatctccgg gaaccagaac
    24181 tggacataaa cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccaa
    24241 taagaccagg cggtgagata accaaagaac gggctcatat aacgatgcgc ataaacggcg
    24301 aacgaaccgg taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaag
    24361 acgaatagcc cggcgatgat ataggccaac aatacggatg gcccggccca tttcaaggta
    24421 ctggcggccc ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata
    24481 tgtcgagctt ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt
    24541 gtgtttgcat gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat
    24601 gttttcgtaa ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa
    24661 taaaaaagca gacaggcgac ggagtgacca ctccgtcgct ttacaaagag aggaaaatca
    24721 gaggttgccg gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg
    24781 gctcggctgc gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg
    24841 tgcgcgtttt acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc
    24901 atctggatgc accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc
    24961 aacggtgacg atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc
    25021 gggtttaaaa taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt
    25081 agcttcagtt tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc
    25141 tttgcctgcg cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg
    25201 cgcctgcggg tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa
    25261 ttcggcagcg ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc
    25321 ggcattaatg gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag
    25381 ggcgtgctgc atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc
    25441 cgtggtgttg ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt
    25501 ttatgaatga gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat
    25561 gcgccgaaga ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt
    25621 gccagcacga tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta
    25681 tagcgattag tctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcagt
    25741 ccaaccacaa tcgccccgag tgggatgaac aacgcgccgc ccatcaccac cagtgagcct
    25801 ataagcgtag gcgagatcgc cagtccggag tggttattca gcacttccca ggtaaagtag
    25861 ttggctgagt tcagcaccat actcgggcga cccggccaca gccaggaagg gataaagaca
    25921 tagaaatcgc ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac
    25981 agcaacgcca gattctccca tggcgagaag gtgtcgcgag tgagatagag aaacgtatag
    26041 aacgcttcat cgccgctcac attcattcca tagcgtttta gcgccagcca gaacatgccc
    26101 acaatcccca gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg
    26161 ccaataaaca ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc
    26221 atataagtca gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgcta
    26281 tcctggcgca gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt
    26341 aacgccacgc cggagacttc actggagaag atctgactgt aggagttaag ccggaacagc
    26401 aaaaagccgt tgtgcatgaa gaagatgccg acgcttacca gcgcgatgcc catcaggatc
    26461 acccacgtaa gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca
    26521 gcaacgcgtt tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc
    26581 gcagaaagca acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat
    26641 accagcacgc tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag
    26701 aagacattga agttaaagcg cacacggcga aactcaaacc aggtcagcgt ggcaataaac
    26761 agcgtgcaga gcagccagac aacaaacagg ccactgaatt gcagcagact catgcgacct
    26821 ccccggcggc aatcgccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg
    26881 caatggtgtt tttatccacc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt
    26941 tgagatcgtc ggtagtaaac agcaccggca aatgctgttc cgtcatatcc tgccagaacg
    27001 gattttcccg gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa
    27061 tgccctgctg gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat
    27121 aggcgtcaaa ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca
    27181 gccccgcctg acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca
    27241 ccaccacttt taccgtatcg ccaaattgct gatgaacggc gcacaaggca gcaatatggt
    27301 cattgctgcg gtcaccggag ttccccacca gaatggtcat tttcccttca cgttgccgat
    27361 cgttcgccat cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc agttcgcccc
    27421 gcacctttgg atgcgttttg gcaaaaaagc tcaaatcgcc gcgggcggca aatacacagc
    27481 cgactcgctt ttgcgccagg cgacgtagtg ggtaaaaaag cttatatctc aagccactgg
    27541 aaagctcata caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac
    27601 cactcagcag agccagccac agtgtgggat tgaactgacc gtggaagaaa aaacgctgct
    27661 gacggttagc ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa
    27721 attgcacaga aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa
    27781 actcgcgcgc gtgctcgctc gtcgcagcca gcgcgtcatt gaaaaaccgc aaaacggttc
    27841 ggttatggtg agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg
    27901 ccagagtaaa aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc
    27961 ctgcgccgcg cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa
    28021 agtgaactgg ctgatttccg ccagaatata aaaccgcaat gacgctttgg cgatcaccag
    28081 ataaccaaag acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag
    28141 atcgcgcata gcggtaaatt tattcgacaa cagcagccag atagcaaaat cacgcagcag
    28201 ccagacggta aaactcgccg ccgccactgc cggtaagacg aatttcagcg atttaaccac
    28261 ttcccgggtg atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct
    28321 gaacgatgcc gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat
    28381 ccccacttca tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac
    28441 aggcaaggtc accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag
    28501 accgttatcc cagctgggtt tcagatagct taacgggatg acaccacgtt tgatcaacat
    28561 gatagcggca ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc
    28621 ataaccgccc aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat
    28681 cagcgataac gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt
    28741 gccccaggcg atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt
    28801 attaccaaac aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag
    28861 cgtagagaaa ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg
    28921 attatcatgg tactgggcaa cgtatttggt tacaccgtta aagatgccag ctccggcaag
    28981 cacgccgagc acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc
    29041 aaatgacaca gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact
    29101 ggccgccgtc cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag
    29161 tcgcaattac cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca
    29221 ggcgctcgct ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagcgttccc
    29281 ccgcagggca accgtgcagc ggaatgtaat ggaacaccgc catgatttcc gcttctttca
    29341 gaaagttaat caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg
    29401 cgttctgcac gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag
    29461 gcgctaacgc atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg
    29521 ctgcttccag ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc
    29581 caatatcgcg ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc
    29641 ccttttcacg gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc
    29701 cgccttcacc acccgccgta tagtttttgg tttcatggaa gctaaagcag ccaatatgac
    29761 caatggttcc cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcta
    29821 ccacaaacag attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac
    29881 ccgcgtaatg gaccggcacg ataacgcgcg ttttgtcggt gatcgccgct tcaatcagcg
    29941 tttcgtcgat gttcatggtg tccgggcgaa catccacaaa aacgattttt gcgccacgca
    30001 gcacaaaggc attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag
    30061 gctggatatc gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta
    30121 acactttggc gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc
    30181 catcgccaca cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca
    30241 ccaccggcgg tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt
    30301 tctacattcg caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc
    30361 gccacccgca aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc
    30421 tcagcaccta caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc
    30481 cgtaaagaga cataaccacg aataccgccg gacgccgcac gtaaaatcag acattgatga
    30541 tcaaaggtgc cgcgcacggc attttcaatc cactgtgcat aaaagcgtct gctggcgtca
    30601 ggcgcatacc acggcgcacg aaaacggctt tgcgcaaatg cggcgctggc taactgacgt
    30661 aatgcgggaa tatcggtctc ttgtgccact acagcaccac tatcactgac attgttcacg
    30721 ggtagtgcca aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc
    30781 agttcacccg tatttgatgc cgcaattttg gcctgcaccc gtgaccacgg cgctaacacg
    30841 tctggcgtca ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag
    30901 aaggcgtttt cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag
    30961 cagatattgg ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc
    31021 atcgagccag ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg
    31081 tttttctact gtctgcacaa aggtactggc ttcaatcagg ctgtcgtgag tgccagtgtc
    31141 cagccaggca aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat
    31201 ctggttgatg gaggtaatct ccagttcacc acgctccgac ggcttcacct gctttgcgta
    31261 ctccacgact ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg
    31321 ttttggcttt tcttccagcg agatagcgcg gaaattgtcg tcaaactcca ccacgccaaa
    31381 gcgttccggg tccatcacct gatagccaaa taccgtcgcc ccttccgtgc gcgccgcaac
    31441 atgacgcagc ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca
    31501 agaaggttca ccgttgagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg
    31561 gctgggctgt tcggcgtatt ccagctggat accgaactca ctaccatcgc ccagcaggcg
    31621 ctggaaataa cctttatctt ccggcgtagt gatgatgaga atttcgcgga taccggccaa
    31681 catcagcacc gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt
    31741 cgatacgccg cgcgtaatcg gatgcaatcg ggtgccggaa ccgcccgcca ggataatacc
    31801 tttcatgttt gcctccgctg aaaattagcc tttcagacct aaacgctcgc cctgatagct
    31861 gccgtcctgc acctgcttcc accagctttc attagccaga taccactgca ccgttttacg
    31921 cattccactt tcaaaggttt cctgtggcag ccaaccaagt tcacgggcaa ttttcgaagc
    31981 atcaatggca taacgcagat catgccccgg acggtcagcg acaaaggtga tcaagtcacg
    32041 atagtgcacc acgccgtgcg gcttgttcgg agccagttct tccagcagct cgcaaatggt
    32101 ttccacaaca tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac
    32161 tttcccggtg gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc
    32221 acggatttgc tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat
    32281 catcagcggg atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt
    32341 gatcagcgtc ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc
    32401 ttttgacgcg gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc
    32461 cgtcgagtgc aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga
    32521 ttttttatct tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt
    32581 cccgacaatg ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct
    32641 ttcggctgcc agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc
    32701 tgcccgatcg cagatatcaa ctttctcaaa ggcaaagtgc tcgctttgcg cgaccggtgc
    32761 cagcgacatc aggtttccgg cgtaggtcag cttatcgacc actaccaccg catcgctcgt
    32821 ttcgttgatg atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag
    32881 aatttttctc atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg
    32941 tcgccattga taactttgaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt
    33001 gccagcgcct cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg
    33061 tggatgttag gctcaacaac cagcgtttcg ccgctgtgcc actgggcgat cagttcagcg
    33121 atttccatcg ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag
    33181 caggcgattt tcagttcact ggcgcgttta tcggtagcag caaggcagtc ggcaaccgcc
    33241 gctttcacct gatcgataac ccagaacggt ttgtgatcgt taacttcgcg cgcggtacgg
    33301 ataagccgcg cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag
    33361 tgaccgccca cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg
    33421 cgaatcagtt cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca
    33481 aaagcgatat tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc
    33541 gagttagtga cgacacactc accttcgagg aaaattttgt acagttcgct ggcgcgggtc
    33601 gaacaaaccg gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc
    33661 tggcccggta acacgcgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc
    33721 tgctgtggga aagtgagatc cggacgcatc tctgctaacc attctgccat cttctcggtt
    33781 gaccccaccg gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca
    33841 atggagcgag cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc
    33901 gtgggtacag caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa
    33961 ccgccttcta cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg
    34021 ccacgattga tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc
    34081 cgtgaggcaa acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg
    34141 gtcgcaaaac tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg
    34201 cctgaccatc accatacggg ttatgggcgc ggctcatagt ttgatattcg ttttcgtctt
    34261 ttaaaagacg cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg
    34321 tacccgccgt caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc
    34381 ccagcgaagg cgcttcttcc tgaatgccgc ctgagtcggt caaaatcagc caggcgtggt
    34441 tcatcagcca gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat
    34501 gccccagaat gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa
    34561 tctggatgtc ctggtgcgtg gtggcgatgt ctgccagcgc gtggcagatt tcttcaaagc
    34621 cacgaccgaa actttcacgc ctgtgaccgg tcaccagaat catcttttta tcggggtcga
    34681 taaacgggta atttgccgcc agttctgaac gcagcgtgtc gctgctcatc acctgatcac
    34741 gcacccataa cagtgcatca atgactgtat taccagtaat gaagattcgg ctatccgcaa
    34801 cgttttcacg cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca
    34861 gatgcccggt caatgtacgg ttagcctctt ccggccacgg cgaatagaga tcgcccgtgc
    34921 gtaaaccagc ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg
    34981 ccagcgtcgt tgttgtgtcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa
    35041 taggttttag cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca
    35101 ttatgttgag atcgtagtca ggtacaatgg aaaagagttt cagcacctga tcgagcatct
    35161 cccgatgctg cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat
    35221 gcaccaacgg agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca
    35281 catcgattct cttcgaataa gcggcgagcg cctttgcgct cgcagcagca gtgttgctat
    35341 ttcgagcaac ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac
    35401 ataatcatca ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc
    35461 aaatagcgat aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac
    35521 atggcccgat tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc
    35581 agtcgagcct ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg
    35641 gcaggcacat ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc
    35701 tcaatgctgt tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc
    35761 tgagctttca tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg
    35821 ctggctgcac gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc
    35881 gcggtttcgg caataagctt cacgctgtca ttgaccgcgc gggtaaagtc tccggggata
    35941 aactggatgt tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc
    36001 atccgctgct tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc
    36061 agttgcataa caaactcttt ataggcttcg tccatgaccg atggttggtc ggcagaagcc
    36121 atgtttgaac gggcatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc
    36181 atattcaccg ttggacgatc ggtaatcgcc gtcgcgctcc actcttgtcg agcaaaaaaa
    36241 gtatacgcca gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca
    36301 gcccacaagg tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt
    36361 ttcccaggca ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac
    36421 cacgatttct acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac
    36481 gcttaatgca atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact
    36541 ccgggacaaa atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa
    36601 gggtaatcag cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa
    36661 tatgctgacg gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg
    36721 ccaccatatc cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat
    36781 gggttttgcc ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac
    36841 tgcccgcatc acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga
    36901 tgtatggcag gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt
    36961 cgaaccacaa aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc
    37021 catcaatgcc atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca
    37081 ggaagtaacc aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac
    37141 tgagataaag tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac
    37201 ggatttttac gctgatatca aaacggtcat ccagcgcgcc aatgaaaaca agcacaccgg
    37261 cacaagcgag atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc
    37321 aaatccctgc gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc
    37381 ggaagtttgg tttatccact aaaccgactt tttttgccac tttacgggca aaaaacagaa
    37441 acagtgtcgt gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa
    37501 tgcatgctct cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag
    37561 ggaaagtccg ttccgaaccc accagtcctg tgcaaaaatt aaccatttga tatgcaaatt
    37621 gcataacatg ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt
    37681 acagatataa aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta
    37741 tgagcgtttc atcatttcga agaaatcgtc attggtctta gtcatggcca gtttattaat
    37801 gaggaattcc attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat
    37861 tttctgcagt tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta
    37921 gtcgatagcc gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt
    37981 gcctgtacct ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag
    38041 cgccgtcgcg ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccgaagaa
    38101 gcgtttcgga cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc
    38161 cggaacaaca gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac
    38221 gtctttcttg tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac
    38281 gtggcgagat gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg
    38341 catctcggtt acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg
    38401 gtggttgtaa gcaatgctct gagcaatgtt ctgcagcagc atagttttac cggctttcgg
    38461 cggtgccaca atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg
    38521 agcggttaaa tcttcagtag aaccgttacc acgttccata cgcagacgag agtttgcgtg
    38581 cagcggggtt aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt
    38641 aacttcgtta actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt
    38701 accagagatg gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta
    38761 gatgtcatca ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc
    38821 ctgcaatatc tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt
    38881 caggatggca aaaataatgt cctgcttacg catacgagcc aggttttcca gccccatatt
    38941 ttcgccgaga gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt
    39001 ggtgtgagtt cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg
    39061 atcatccatg cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga
    39121 gtacgcagaa ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg
    39181 catttaagat aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt
    39241 taatacgaag tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg
    39301 gggcacccga acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga
    39361 cctttagaca gtgcacccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga
    39421 gtcgggatac cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt
    39481 tttgcaacgg tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc
    39541 attttgcacg gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg
    39601 agtacatccg tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac
    39661 tccacaggaa taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag
    39721 gttgacttta tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac
    39781 actatgagca aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag
    39841 gttgtagaag cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc
    39901 cttccgctga cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa
    39961 acgatggcgt ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat
    40021 cgcaaggtga atcaaccacg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag
    40081 atccatgccg acgcagaacc gctggcggaa gctaccggcc tgaagctggg tctggcttac
    40141 ggtggtgatg gctacgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt
    40201 ggcactacgg ggcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt
    40261 caggtggtgg tactggacga agccgatcgc atgtacgatc tgggctttat taaagatatc
    40321 cgctggctgt tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc
    40381 acgctttcgt accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt
    40441 gaagtggaac cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct
    40501 aacgaagaaa aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg
    40561 attattttcg ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat
    40621 ggtcatcgtg tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt
    40681 gatgaattta cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt
    40741 ttgcatattc cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat
    40801 tacgttcacc gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg
    40861 gcgtgtgaag agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt
    40921 ccggtaagca aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc
    40981 acgcgcccac gtacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt
    41041 tcaggttaaa taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa
    41101 tgaatcccga tgagcatact gtagtaagtg attcaggtga aagaatgcag ccaacacaga
    41161 gacagattga aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct
    41221 cggttcgaat agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct
    41281 gacgcgaata aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgccctgtc
    41341 taatgaagca atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga
    41401 tatccctccc tcgcaaattc gcgttgtcgc tacggcgaca ttacgtcttg ccgtcaatgc
    41461 gggtgatttt attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg
    41521 tgaagaggaa gcacgtctga tttatcaggg cgttgctcac acaactggcg gtgccgatca
    41581 gcgcctggtg gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca
    41641 aaccacctcg ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc
    41701 cgatcgtaat ctggggcagg aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt
    41761 acgtccggtt gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg
    41821 caccgtgcag gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct
    41881 ggaaaagttg cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga
    41941 gattgacggg ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat
    42001 cgccattttt accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga
    42061 aggcctggtc tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacgct
    42121 gcgtaatatt cagcgccgct ttatgatcga tattgatcag gcgcagcgcg tagccaaagt
    42181 tgcggctaac ttcttcgatc aggtggaaaa ggaatggcat cttgaagcaa taagccgcga
    42241 tttgctcatc agcgcctgtc agcttcatga aatcggcctg agcgttgact ttaaacaagc
    42301 gccgcaacac gctgcttatc tggtgcgtaa tctggatctt cccggtttta cccccgcaca
    42361 gaaaaaattg ctggcgacgc tactgctcaa ccagactaat ccggtcgatc tctcatcgct
    42421 gcatcagcaa aatgccgtac cgccgcgcgt cgcagaacaa ctctgccgtt tactccgcct
    42481 ggccatcatt tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc
    42541 taaccatgaa ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg
    42601 taaagagatt attgctcagg aaagccagtg gcagagctat gtccactggc cgctggaagt
    42661 gcattaatga gtaagtgccg gatgcgatgc tgacgcatct tttccggcct tgattatttc
    42721 cctcgttttg ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc
    42781 cgttcttcgg cgctgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc
    42841 tccagcaaaa agcggctcgg ctccgggcgc accagctcgc cgtactgacg gcgttcttta
    42901 cacagcgtaa aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc
    42961 tcctcatcga tattatcttc atcgatgctg ctctggtgcg gcaaaaaccc ttcttccata
    43021 ccgaccatgt agacataagg aaactccagc ccttttgacg cgtgcagagt catcagttgc
    43081 acctgatcca gctcttcttc actctcacca cgctccatca tgtcgcgcaa agtaaagcgc
    43141 gtcaccacct gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc
    43201 atccagctaa acagttggtt gacgttcttc atgcgcattt cggcggcttt cgggctgggc
    43261 gatgtttcgt acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca
    43321 atcggctccc gctccgccag acgctggatt tctgccaacc agtgagtaaa gcgggtcaat
    43381 gcttcataac cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac
    43441 atgcttttat tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc
    43501 tctctcttcg gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc
    43561 acgcgcagat aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca
    43621 gaaattttgt acgggatgcg gttttgcatc aggaattttt caaacactcg cgactgatgg
    43681 ttaccgcgat aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca
    43741 atcagttcac cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaacttta
    43801 agctctgtgc cgtaacccag ttcggagaac agacgcttct caaagacgtg cgggttattg
    43861 gcgatcagga tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc
    43921 ttaatcaccg tcagcgccgg aaaatcctga ctcagcagca ccaggttttg tggacgtgcg
    43981 ccgcgccagg agtagatcga ctggtcgtca tcgcccacca cggtaaagcg cgcgcggctg
    44041 cccaccagca gtttcaccag ctcatactgg ctggtgtttg tgtcctgata ctcatccacc
    44101 agcagatagc gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagcagc
    44161 aacgtcggca ataaaatcag atcatcgaag tcgagaacat tgcacgcttt caggtgtgca
    44221 tcatacagcc cataacaatg ggcaaaaata cgatcccgct cgccctttgc ctctgccgcc
    44281 gcctgcgccg gagttttgag atcgttcttc cagttggaga tggtcgaaat cagctgttgc
    44341 aggagaactt tgtcatcttc aatcagcccc tcggtcaact ctttaagcaa agcaagctga
    44401 tcggtatcgt caaacagcga aaagttcgcc ttcatcccaa gcgccgcata ctcgcgtttg
    44461 atgatatcca accccagcgt gtgaaaagtg gagatcatca gcccgcgcgc ctctttgcga
    44521 cccagcgtct gcccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc
    44581 gccgcgatat gtcgcgcctg ataaccgcaa ccgcggatca gatgggcgat tttattggtg
    44641 atcacacgag ttttaccgga acccgcgccc gccagcacca ggcaggggcc ggtaacgaat
    44701 tcgacagctt gttgttggcc ggggtttaga cgcataggtg tattgctcaa tcttcgaacg
    44761 gggggaggat tgtagcagaa tcgcatttga atgtgaccgc gtgtaagccg ctaaccggtt
    44821 ggctgcggat gtttaaatca gagagagaag accgaaatcg gagttcatcg tgaatccatt
    44881 agacttagaa aatatcgggt cgtaacgttg tgttattact ttttcagagt ttactaagga
    44941 gaggtttact cgtggcgatt aatttcagcc ctaaggttgg tgaaatacag gaatgcaact
    45001 ttggtaacta tcctgttatc cagaatggac agttcagcac cacgtattac gatggccgca
    45061 ttcctcctga aatgataaaa aacaggttag ttgtcgtgct caacggaaaa attaatggca
    45121 acgcatgtat cgtcgttcca ttatcaacca ctcgcgatca tgacaagtta aatcgtggaa
    45181 tgcatgttga aatagccagt aatgttatca atgtcctgca attctttgat cagcagattc
    45241 gttgggccaa agccgacctg gtacaacaag tcagtcgaaa ccgattaaat agagcaagga
    45301 cttacagagg gtatcttaat caatgtttgc cacatgagtt ggttgcagac atacagaggg
    45361 cggtcatcaa gtcaatcaat gccatatcgc tcataaattg acaataaaat aaccatcccc
    45421 gataatgact ttgtagccca tagaggccct aagttttctc cccttgaggg acctgcgaag
    45481 cccggatttt ccgggctttt gccatatatg taccagcccc tccctgactg acaattacgt
    45541 tagcatctgc ggatcatgct aaaatcgccg cctgacaatt attcactcaa ggcacgatca
    45601 tggcaaaaac agcagcagca ctgcatatcc ttgtaaaaga agagaaactg gctctggatc
    45661 ttctcgagca gattaagaac ggtgccgatt tcggcaagct ggcgaagaaa cactccattt
    45721 gcccatcagg caaacgcggc ggtgatttag gtgaattccg ccagggtcag atggttccgg
    45781 cgttcgataa agttgtgttc tcttgtccgg tactggagcc gaccggcccg ctgcacaccc
    45841 agttcggtta ccacatcatt aaggtgctgt accgcaacta atagcaaggc cttctccagg
    45901 agaaggcctt gagtgttttc tccctctccc tgtgggagag ggtcggggtg agggcatcag
    45961 cgcgcactta acccgcaaca gcaatacgtt tcatatctgt catatagccg cgcagtttct
    46021 tacctacctg ctcaatcgca tggctgcgaa tcgcttcgtt cacatcacgc agttgcgcgt
    46081 tatctaccgc gccttccgga atagctttac ccaagtcgcc cggttgcagc tctgccataa
    46141 acggtttcag caacggcaca caagcgtaag agaacagata gttaccgtac tcagcggtat
    46201 cagagataac cacgttcatt tcgtacagac gcttacgggc gatggtgttg gcaatcagcg
    46261 gcagctcgtg cagtgattca taatatgcag actcttcaat gatgccggaa tcgaccatgg
    46321 tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg cctttatcga
    46381 agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag gcggttttgc
    46441 cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc gccatcatac
    46501 cggaggagaa ctcgccggag atgatgtcgt ccatatgttt ctggaacagg ggtgccatga
    46561 tctctttcag ctgttcagac agcgcgtaag cacgcagttt cgccgggtta gagagacggt
    46621 ccatcatcag ggtgatgccg ccctgcttca gcgcttcggt gatggtttcc caaccgaact
    46681 gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg tcgaagcaca
    46741 gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc aggtcagatt
    46801 tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg gttgctgccg
    46861 cccatgcttt agcgatcgcc ataccttcgc ctttcggatc gttttccggg tgaacggcaa
    46921 tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg ccagggcatt
    46981 tcggcgcaac catgactacg gtgatatctt tacggatctg ctcgcccact tcgacgatgt
    47041 tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt acggtgcgca
    47101 ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc tgcgggatca
    47161 gttcttcgta agtaccaact ttaaaaccgt tttcggtcgc tttacgccag gacgcgcgct
    47221 tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa tcacgcatgt
    47281 tcaggccctg gttcagcccc tgtgcgccac agccgacgat aaccactttt ttaccctgaa
    47341 ggtagctcgc gccgtcggcg aattcgtcgc gccccataaa gcgacatttg cccagctgtg
    47401 ccagctgctg gcgcaggttc agtgtattga agtagttagc catggtgatt cctcgtgatg
    47461 ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat gacaggaaat
    47521 ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac gtcaacattg
    47581 agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc
    47641 cattttggcc gcagcgcgcg ggcgatgcac gttagcccat ccacgctctc acggcagatt
    47701 cagcgcctgg aagaagatct tggtcagccg ctatttgtgc gcgataaccg cacggtaacg
    47761 ctgaccgaag cgggcgaaga gttacgcgtt ttcgcccagc aaacattgtt gcagtatcag
    47821 caactgcgcc acaccatcga tcagcaaggg ccgtcgctct ctggcgagtt acatattttc
    47881 tgctcggtga ccgccgctta cagccatcta ccgccgattc tcgaccgctt ccgcgcggaa
    47941 cacccgtcgg tggagatcaa actcaccact ggtgatgcgg cagatgcaat ggaaaaagtg
    48001 gtcacgggag aagcggatct ggcgattgcc gggaaaccgg agacgctacc cggcgctgtg
    48061 gcgttttcga tgctggagaa tctggcagta gtgctgattg cccccgcgct gccctgcccg
    48121 gtgcgtaatc aggtatctgt cgaaaagccc gactggtcaa cggtgccgtt tattatggcc
    48181 gatcaggggc cggtacgccg ccgcattgaa ctgtggtttc gacgcaataa aatcagcaac
    48241 ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt ggcgctcggc
    48301 tgtggtgtgg cgctgttgcc ggaagtggtg ctggaaaaca gcccggagcc tgtacgcaac
    48361 cgggtaatga ttttagatcg cagcgatgag aaaacgccgt ttgaactggg cgtgtgtgca
    48421 caaaaaaagc ggctgcatga gccgctgatt gaggcgttct ggaggatttt gccgaaccac
    48481 aaatgacgtt gtcgcgcggg taggcctgat aagcgaagcg ctatcaggca tttttcccta
    48541 acccgccaaa aagaacctga acgccggatt attggtttcg tcgtggcaat cgtagcccag
    48601 ctcatttagc cgggtttcga aatccggttc atggtcgccg agttcgaacg ccgccagtac
    48661 gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtgcgtacc
    48721 cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga agctgtagag
    48781 gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat gcaacttcgc
    48841 catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca tctgcaaaat
    48901 ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc aggcgttttt
    48961 ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca gttggcagaa
    49021 tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc
    49081 gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt tggcaccgga
    49141 aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt ttttcattcc
    49201 cgccagcgcc agcgcgccag agggttccgc caccgcgcgc acatcttcga ataaatcctt
    49261 catcgccgca cagatcgcat cactatcgac ggtgatgatg tcgtcgagat actcctggca
    49321 taaacggaag gtttcgtcac cgatgcgttt taccgctacg ccttcagcaa atagccctac
    49381 gcgcggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc
    49441 cgcttctacg gcgatcactt tgatttgtgg catcagttgt ttgatcagca ccgcaacgcc
    49501 cgccgccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg cgtcctgctg
    49561 gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg
    49621 cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttagctttcg cttcgtcaaa
    49681 gttcgcgcca tgaagcagca cttcgccgcc gaatccacgt accgcatcga ctttgatgtc
    49741 ggcggtggcg gttggcataa cgatcagggc cttcacgcct aaccgcgcag aagaaaacgc
    49801 gacgccctgc gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt tttgttcttc
    49861 cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca ctggctggcg
    49921 atcttcgcgc ttcaccagaa tgacgttatc aagacgcgac gacagttttt ccattttttg
    49981 tagcggcgta acctgcgccg cctcgtaaac cggcgcgcgc agcactgctc ttaaatattc
    50041 ggcaccttcc ggagcaccgg acaggggttg cgagtcagcc attattaacc ccccagtttc
    50101 gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta ggcgcgcagg
    50161 gcaaaggaaa cctgacgttc acggtttttc ggcgtccagg ctttgtcacc ccgagcttcc
    50221 tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat gccacggttc
    50281 gggatgtcga tagcgatcag gtcaccatct tcaatcagac caatgctgcc gccgcttgcc
    50341 gcttccggtg agacgtggcc gatggaaaga ccagaggtgc cgccagagaa acgaccgtcg
    50401 gtgatcagcg cacaggcttt accgagaccc attgatttca ggaagctggt tgggtagagc
    50461 atttcctgca tgccagggcc gcctttcggg ccttcatagc ggatcaccac cacatcgccc
    50521 gcgacaactt tgccaccgag gatcgcttct accgcgtcgt cctggctttc gtacactttc
    50581 gccgggccgg tgaatttgag gatgctgtca tcgacgcccg cggttttaac gatgcagccg
    50641 ttttctgcga aattaccgta gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc
    50701 agcgagcgga tacagccatt ggcgcgatcg tcatcgagag aatcccaacg gcaatcctgc
    50761 gagaatgcct gtgtggtacg aatgcctgca ggacctgcgc ggaacatatt ttttaccgcg
    50821 tcatcctggg tcagcataac gtcgtattgt tccagcgttt gcggcaacgt caggccaagt
    50881 acgtttttca catcacggtt cagtaacccc gcgcgatcca gttcgccgag aataccgata
    50941 acaccaccag cacggtgaac atcttccata tggtatttct gggtgctcgg cgcaacttta
    51001 cacagctgtg gaaccttgcg ggaaagctta tcgatatcac tcatggtgaa gtcgatttcc
    51061 gcttcctgcg ccgccgccag caggtgaagt acggtgttag tcgatccacc catcgcgata
    51121 tccagcgtca tggcgttttc aaacgccgct ttgctagcaa tattgcgcgg cagtgcactt
    51181 tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgtttacc ggcattgagg
    51241 aacaactgct tacggtcggc gtgggttgcc agcagcgagc cgttgcccgg ctgcgacaga
    51301 cccagcgctt cggtcaggca gttcattgag ttagcggtaa acatcccgga gcaggagccg
    51361 caggtcggac acgcggaacg ttcaacctga tcgctctggg agtcagaaac tttcgggtct
    51421 gcgccctgga tcatcgcatc aaccagatcg agtttgatta tctgatcgga cagtttggtt
    51481 ttcccggcct ccatcgggcc gccggaaaca aagatcaccg gaatattcag gcgcagggaa
    51541 gccatcagca tccccggggt gattttgtcg cagttagaga tgcagaccat ggcgtcggcg
    51601 cagtgggcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggcagtgaa
    51661 taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct
    51721 ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga
    51781 tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat cggcttaccg
    51841 aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc catattacga
    51901 ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc agtgtctgtc
    51961 tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa
    52021 ccagccccat ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctgaatgcg
    52081 tttggtaacc gggccacaac ggccttcgcc aacctgaata ccgtctacgc tgcgcactgg
    52141 cgtgatttct gccgccgtac cggacataaa cacttcatcc gccaggtaca gggattcgcg
    52201 cgacagaacc tgctcacgca catcaattcc cagctctttc gccagtttga tgatggcatc
    52261 acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca caccatcttt
    52321 cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat ccagcgcgat
    52381 accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag agaggtagtt
    52441 accaccggct tttgccgccg tcgggatggt gtttggtgct gcgcggttcc aggaggaaac
    52501 catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc
    52561 agcgataatc acgtcggttg agtatcccgc tggcgggttt actcccatgc caacatcacc
    52621 gacgaagatc agcggacgga tataggcgct ggtgagatta tttttgcgga tcacgtcacg
    52681 acaagcttcc atcagctcat caatgctctg tgaaaccggg aagcgataga ttttggcgga
    52741 gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgtgagtc
    52801 gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg
    52861 caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt
    52921 cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc
    52981 tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt
    53041 cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg
    53101 cagccatatt cattgagcag acgtggaaac cacgatgacg caccacgcgt aaaacacgtt
    53161 ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc atgataattt
    53221 ctccaacatt tctgaattac tggcgccagg cggcaccagc ggccagacgt tctcaagttc
    53281 gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt cgagtgccgc
    53341 ttcaacctgg tctttacggg tgatgtgttg gccagggatg ccgaaggcgc tggctaacat
    53401 gaggaaatcg gggttatcag taagggtggt ttcgctgtat cgttcctgaa aaaacagttg
    53461 ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa
    53521 ctgcttgcgt tttacggtgc ccagctcttg cacattcatc atgaaagagc cgtcaccgga
    53581 gatacagacg acagtatcgt tcggtcgtgc gacttgtgcg ccaaccgccg ctggtaaacc
    53641 gaaacccatg gtgcctaagc cgctggaggt aatgaaattt tccgggcgag tgtgtgcgat
    53701 gtgctgcgcg gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg
    53761 tttacgatcc gacagttgtt ttaacaacaa tggcgcgtag atagcgtcac cgggatggtc
    53821 gtaacgccag gcatgttcat cacgcagctg cgcgcagtgt agctgccagt cattgatatt
    53881 taacggctgc tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc
    53941 ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg
    54001 tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag
    54061 caagtcgcac tcctgcaccg cgaagttcgc cgctttggtg ccatgcattc ccagcatgcc
    54121 cagatagtac ggataatctg cttcaactgc gcccagccct ttcagcgtgc aggtggcagg
    54181 catttttgtg gtagcgagaa attctcgtaa agcaggaact gcctgcgcca tacccacgcc
    54241 accaccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcaac
    54301 ttcggcatgt gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccaggtcacc
    54361 gctggctagc tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct
    54421 ggcaacgtcg aatgcttcag ccataatgcg cggcaactct tccagcgact gcaccagaaa
    54481 gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa atgcgtccgt
    54541 gccgataaac ggtgcggaca cttgaccggt gatggcaaca acagggatag aatctaacag
    54601 tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac
    54661 gccagttttg ccggtagcac gggcataacc gatagccgcc attgccgcac cctgctcatg
    54721 tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc
    54781 gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat gtaccaccca
    54841 ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg
    54901 ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaacccc cggacctttc
    54961 ggtgcggggg tcttagttcg ttaaggcttg atctctaagc ctttcctcgt ccaagtgcag
    55021 ccccgcacgg tgggataata atcaccacca cgctaatcac gaccaggcta atcactcgta
    55081 gaagggctgt cattttgtct tttcttgcat cttgttcgaa ggaatgccta aagagttacc
    55141 atagattttg cgaatagcac aagatatttt ttggccgatt tttatgatgc gtttaataat
    55201 ttcaatagaa attgttgttt tttaaaaaga aaatagtgag tgaaaaaaaa ttcaatttca
    55261 gtatgaatgt tgttcccttc ctcgtagttc atcctttgtc cgctgcattt ttgcgagcat
    55321 cattccaccg ccagaacaac tcaacttcac ttgcacaaat aaacaggaat cagccactaa
    55381 aattcacgaa ttagcgggct acagcagcga tatcttatgc acataatagt gactcaagga
    55441 gggcttatgt cactgtcaat tgttcatacc cgcgcagccc tgggagtaaa tgcgccccca
    55501 atcactgttg aggtacatat cagtaaaggt ctacccggct taacgatggt gggcttacca
    55561 gaaacaactg taaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa tagcggatat
    55621 gaatatccgg cgaaaaaaat caccatcaac ctggctccag ccgatctgcc aaaagaaggg
    55681 ggacgatatg atttacctat cgccattgcg ttgctggcgg cctcagaaca gcttacagcc
    55741 aataagttag atgaatatga attagtcgga gaactggcgc ttacaggcgc tctgcgtggc
    55801 gttcccggcg caatctccag tgcaactgaa gctattaagt cgggcagaaa aattatcgtc
    55861 gcgaaagata acgaagatga agtggggcta attaacggtg aaggatgcct gatagccgat
    55921 catctgcagg ctgtctgtgc gtttctggaa ggtaagcacg ctctcgaacg cccgaaacca
    55981 actgatgcag tatcccgggc gctacaacat gatctcagtg atgttgtcgg tcaggagcaa
    56041 ggaaagcgag gactggaaat taccgccgct ggcgggcaca accttttact gattgggccg
    56101 ccgggaacag gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc agatttaagc
    56161 aatgaagagg cactggagag cgctgcgata ttaagtctgg taaatgctga atcagtacaa
    56221 aaacaatggc ggcagcgccc gttccgctca ccgcatcaca gtgcgtcgtt aactgcgatg
    56281 gtgggcggtg gtgcaattcc agggccaggt gaaatttcgt tggcgcataa cggcgtgctt
    56341 tttcttgatg agctacctga atttgaacgg cgtacactgg atgccttgcg agagccgatt
    56401 gaatccgggc agatccatct ttcacgcaca cgagccaaaa taacctatcc agcccgtttc
    56461 cagcttgttg cggcgatgaa tcccagccct accggacatt atcagggaaa ccataaccgc
    56521 tgcacgccag aacagacatt acgttatctc aaccggctct cggggccctt tctcgaccgc
    56581 ttcgatctct cactggagat cccattacca ccccccggca ttttgagtaa aacggtagtg
    56641 ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt
    56701 aagcggcaga ataaactgaa tgcctggctg gatagtccgg aaatacgcca attctgcaaa
    56761 cttgagagcg aagatgcgat gtggttggaa ggaacactga tccatctggg gttatcgatt
    56821 cgtgcctggc agcggttatt gaaagttgca cgaaccattg ctgatattga tcagtctgac
    56881 attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga ccgtttgctc
    56941 atccatctgc aaaaactact gacataaaaa aagggcattt cgcccttttt attaatcatc
    57001 agaatcggtg tagtcttcag caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa
    57061 acgttttgga cgcttaatac gcgtcatata cttggaccac acgcgttctg cttctgtcac
    57121 tggctcacgt tcgccacggc atactgctac gaagagtttc tcttcctcgg taaccggctc
    57181 gcgtttgcca agatccaact cattgaaggc ataaccatga cgctcaagca gttgtgcctc
    57241 tttgatggtg aaatcaccat gacgagagaa tccacgtgga taatgtttat tgtcgaaata
    57301 tcgattagtc gtcgtaaagc tttccgccat cctgcacgct cctaattctt tgaccgagct
    57361 agttatggcg cggagtatta gttacgcttg acagagtgta aaacaaaaca tttaaatcat
    57421 aacgacaaat aattttgcgg agagcactgt ggatacggaa ttgttaaaaa ctttcctgga
    57481 agttagccga acgcgtcact ttggtcgagc ggctgaatcg ctctatctga cccagtcagc
    57541 agtgagcttt cgaatcagac aactggaaaa tcaactgggt gtgaaccttt tcacccgcca
    57601 cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct
    57661 catgagcacc tggcaggccg cccgcaagga ggtggcgcat acctcacgac ataacgagtt
    57721 ttctatcggt gccagcgcct cattgtggga atgtatgctt aatcagtggc tgggacgttt
    57781 gtatcaaaat caggatgccc atacaggctt acagttcgaa gcgcgaattg cccaacggca
    57841 gtctctggta aaacagctgc atgaacgcca gcttgatctt cttattacca ctgaagcgcc
    57901 caaaatggac gaatttagta gtcagttgct gggatatttc actttagcgc tttataccag
    57961 tgccccttca aaactaaagg gagatcttaa ttatctgcga cttgagtggg ggccagattt
    58021 tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtg cccattctga caaccagttc
    58081 tgctgaactg gcacagcaac agattgcgat gcttaatggt tgcacctggc tacccgtcag
    58141 ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac tttcacggcc
    58201 gctttatgcc atatggctgc aaaatagcga taaaaatacg ttgattcgcg atcttttgaa
    58261 aattaacgtg ctggatgaag tgtattaata tgaatggctg gcaaggatgc cggtagaagg
    58321 atttacttcg gagagggtta tttcagataa aaaaaatcct tagctttcgc taaggatgat
    58381 ttctggcagg ggcggagaga ctcgaactcc caacacccgg ttttggagac cggtgctcta
    58441 ccaattgaac tacgccccta attag
//
LOCUS       DAZFWP020000009        58377 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFWP020000009 DAZFWP020000000
VERSION     DAZFWP020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 58377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.009"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            18..734
                     /locus_tag="ACHK0Z_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             18..734
                     /locus_tag="ACHK0Z_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4500593.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINNQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            764..1264
                     /locus_tag="ACHK0Z_RS02610"
                     /old_locus_tag="pgaptmp_000522"
     CDS             764..1264
                     /locus_tag="ACHK0Z_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HGU4500594.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1356..4040
                     /locus_tag="ACHK0Z_RS02615"
                     /old_locus_tag="pgaptmp_000523"
     CDS             1356..4040
                     /locus_tag="ACHK0Z_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HGU4500595.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4037..5125
                     /locus_tag="ACHK0Z_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             4037..5125
                     /locus_tag="ACHK0Z_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HGU4500596.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="ACHK0Z_RS02625"
                     /old_locus_tag="pgaptmp_000525"
     CDS             complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="ACHK0Z_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU4500597.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6994..8406
                     /gene="uxaC"
                     /locus_tag="ACHK0Z_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             6994..8406
                     /gene="uxaC"
                     /locus_tag="ACHK0Z_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU4500598.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8421..9908
                     /gene="uxaA"
                     /locus_tag="ACHK0Z_RS02635"
                     /old_locus_tag="pgaptmp_000527"
     CDS             8421..9908
                     /gene="uxaA"
                     /locus_tag="ACHK0Z_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU4500599.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVVFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9991..10542
                     /gene="ygjV"
                     /locus_tag="ACHK0Z_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             9991..10542
                     /gene="ygjV"
                     /locus_tag="ACHK0Z_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU4500600.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10547..11791)
                     /gene="sstT"
                     /locus_tag="ACHK0Z_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(10547..11791)
                     /gene="sstT"
                     /locus_tag="ACHK0Z_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU4500601.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12190..13155)
                     /gene="alx"
                     /locus_tag="ACHK0Z_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             complement(12190..13155)
                     /gene="alx"
                     /locus_tag="ACHK0Z_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU4500602.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13438..14424)
                     /gene="ygjR"
                     /locus_tag="ACHK0Z_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             complement(13438..14424)
                     /gene="ygjR"
                     /locus_tag="ACHK0Z_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4500603.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VGAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14503..15186)
                     /gene="ygjQ"
                     /locus_tag="ACHK0Z_RS02660"
                     /old_locus_tag="pgaptmp_000532"
     CDS             complement(14503..15186)
                     /gene="ygjQ"
                     /locus_tag="ACHK0Z_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU4500604.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15263..15766)
                     /gene="ygjP"
                     /locus_tag="ACHK0Z_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             complement(15263..15766)
                     /gene="ygjP"
                     /locus_tag="ACHK0Z_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU4500605.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15851..16987
                     /gene="rlmG"
                     /locus_tag="ACHK0Z_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             15851..16987
                     /gene="rlmG"
                     /locus_tag="ACHK0Z_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU4500606.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17178..17261
                     /locus_tag="ACHK0Z_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /pseudo
     CDS             17178..17261
                     /locus_tag="ACHK0Z_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17258..17674
                     /gene="higA"
                     /locus_tag="ACHK0Z_RS02680"
                     /old_locus_tag="pgaptmp_000536"
     CDS             17258..17674
                     /gene="higA"
                     /locus_tag="ACHK0Z_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU4500607.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17719..19737)
                     /gene="fadH"
                     /locus_tag="ACHK0Z_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             complement(17719..19737)
                     /gene="fadH"
                     /locus_tag="ACHK0Z_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU4500608.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20063..22414)
                     /gene="ygjK"
                     /locus_tag="ACHK0Z_RS02690"
                     /old_locus_tag="pgaptmp_000538"
     CDS             complement(20063..22414)
                     /gene="ygjK"
                     /locus_tag="ACHK0Z_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU4500609.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22431..23501)
                     /gene="ygjJ"
                     /locus_tag="ACHK0Z_RS02695"
                     /old_locus_tag="pgaptmp_000539"
     CDS             complement(22431..23501)
                     /gene="ygjJ"
                     /locus_tag="ACHK0Z_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU4500610.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23635..25068)
                     /gene="ygjI"
                     /locus_tag="ACHK0Z_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             complement(23635..25068)
                     /gene="ygjI"
                     /locus_tag="ACHK0Z_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4500611.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25131..25580)
                     /gene="ebgC"
                     /locus_tag="ACHK0Z_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             complement(25131..25580)
                     /gene="ebgC"
                     /locus_tag="ACHK0Z_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU4500612.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25577..28669)
                     /gene="ebgA"
                     /locus_tag="ACHK0Z_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             complement(25577..28669)
                     /gene="ebgA"
                     /locus_tag="ACHK0Z_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU4500613.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRGFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28853..29836)
                     /gene="ebgR"
                     /locus_tag="ACHK0Z_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             complement(28853..29836)
                     /gene="ebgR"
                     /locus_tag="ACHK0Z_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU4500614.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30055..30387
                     /gene="ygjH"
                     /locus_tag="ACHK0Z_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             30055..30387
                     /gene="ygjH"
                     /locus_tag="ACHK0Z_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU4500615.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30429..31808)
                     /gene="ygjG"
                     /locus_tag="ACHK0Z_RS02725"
                     /old_locus_tag="pgaptmp_000545"
     CDS             complement(30429..31808)
                     /gene="ygjG"
                     /locus_tag="ACHK0Z_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU4500616.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32226..33746
                     /gene="aer"
                     /locus_tag="ACHK0Z_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             32226..33746
                     /gene="aer"
                     /locus_tag="ACHK0Z_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU4500617.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNSSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33900..34523)
                     /gene="nfeR"
                     /locus_tag="ACHK0Z_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /gene_synonym="yqjI"
     CDS             complement(33900..34523)
                     /gene="nfeR"
                     /locus_tag="ACHK0Z_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU4500618.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34800..35564
                     /gene="nfeF"
                     /locus_tag="ACHK0Z_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /gene_synonym="yqjH"
     CDS             34800..35564
                     /gene="nfeF"
                     /locus_tag="ACHK0Z_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU4500619.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35618..35693)
                     /locus_tag="ACHK0Z_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     tRNA            complement(35618..35693)
                     /locus_tag="ACHK0Z_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35658..35660),aa:Ile,seq:cat)
     gene            35818..36324
                     /gene="mug"
                     /locus_tag="ACHK0Z_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     CDS             35818..36324
                     /gene="mug"
                     /locus_tag="ACHK0Z_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU4500620.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36403..38244)
                     /gene="rpoD"
                     /locus_tag="ACHK0Z_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     CDS             complement(36403..38244)
                     /gene="rpoD"
                     /locus_tag="ACHK0Z_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU4500621.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38439..40184)
                     /gene="dnaG"
                     /locus_tag="ACHK0Z_RS02760"
                     /old_locus_tag="pgaptmp_000552"
     CDS             complement(38439..40184)
                     /gene="dnaG"
                     /locus_tag="ACHK0Z_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4500622.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40295..40510)
                     /gene="rpsU"
                     /locus_tag="ACHK0Z_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             complement(40295..40510)
                     /gene="rpsU"
                     /locus_tag="ACHK0Z_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU4500623.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40748..41761
                     /gene="tsaD"
                     /locus_tag="ACHK0Z_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             40748..41761
                     /gene="tsaD"
                     /locus_tag="ACHK0Z_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU4500624.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVTAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41804..43267)
                     /gene="ttdT"
                     /locus_tag="ACHK0Z_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             complement(41804..43267)
                     /gene="ttdT"
                     /locus_tag="ACHK0Z_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU4500625.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43316..43921)
                     /gene="ttdB"
                     /locus_tag="ACHK0Z_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             complement(43316..43921)
                     /gene="ttdB"
                     /locus_tag="ACHK0Z_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU4500626.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43918..44829)
                     /gene="ttdA"
                     /locus_tag="ACHK0Z_RS02785"
                     /old_locus_tag="pgaptmp_000557"
     CDS             complement(43918..44829)
                     /gene="ttdA"
                     /locus_tag="ACHK0Z_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU4500627.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45036..45968
                     /gene="ttdR"
                     /locus_tag="ACHK0Z_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /gene_synonym="dan"
     CDS             45036..45968
                     /gene="ttdR"
                     /locus_tag="ACHK0Z_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU4500628.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(45981..46598)
                     /gene="plsY"
                     /locus_tag="ACHK0Z_RS02795"
                     /old_locus_tag="pgaptmp_000559"
     CDS             complement(45981..46598)
                     /gene="plsY"
                     /locus_tag="ACHK0Z_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU4500629.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46703..47071
                     /gene="folB"
                     /locus_tag="ACHK0Z_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             46703..47071
                     /gene="folB"
                     /locus_tag="ACHK0Z_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU4500630.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47162..47983
                     /gene="bacA"
                     /locus_tag="ACHK0Z_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             47162..47983
                     /gene="bacA"
                     /locus_tag="ACHK0Z_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4500631.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48146..49384)
                     /gene="cca"
                     /locus_tag="ACHK0Z_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             complement(48146..49384)
                     /gene="cca"
                     /locus_tag="ACHK0Z_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU4500632.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(49448..50068)
                     /gene="ygiM"
                     /locus_tag="ACHK0Z_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             complement(49448..50068)
                     /gene="ygiM"
                     /locus_tag="ACHK0Z_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU4500633.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            50310..51611
                     /gene="ygiF"
                     /locus_tag="ACHK0Z_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             50310..51611
                     /gene="ygiF"
                     /locus_tag="ACHK0Z_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU4500634.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51634..54474
                     /gene="glnE"
                     /locus_tag="ACHK0Z_RS02825"
                     /old_locus_tag="pgaptmp_000565"
     CDS             51634..54474
                     /gene="glnE"
                     /locus_tag="ACHK0Z_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU4500635.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            54522..55955
                     /gene="hldE"
                     /locus_tag="ACHK0Z_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             54522..55955
                     /gene="hldE"
                     /locus_tag="ACHK0Z_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU4500636.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALVAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56075..56134)
                     /gene="ibsE"
                     /locus_tag="ACHK0Z_RS02835"
                     /old_locus_tag="pgaptmp_000567"
     CDS             complement(56075..56134)
                     /gene="ibsE"
                     /locus_tag="ACHK0Z_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU4500637.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56451..56510)
                     /locus_tag="ACHK0Z_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             complement(56451..56510)
                     /locus_tag="ACHK0Z_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4500638.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(56749..>58377)
                     /gene="yqiK"
                     /locus_tag="ACHK0Z_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             complement(56749..>58377)
                     /gene="yqiK"
                     /locus_tag="ACHK0Z_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU4500639.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
BASE COUNT        13954 a        14922 c        15588 g        13913 t
ORIGIN      
        1 tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt
       61 tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt
      121 ccattaataa tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt
      181 acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg
      241 agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc gctttaccag
      301 ccggtagtag caaaaccgta cgttttgttg cgatggaacc agagcaaaaa gagaaaaatt
      361 atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc
      421 tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc
      481 aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaatcaac
      541 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
      601 agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa tcttgaaagc atatctggct
      661 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      721 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      781 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      841 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      901 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      961 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
     1021 cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt gttccgctga cagtaacctg
     1081 gggcggtgaa gaaattaaag ctgatgcggc aaccactttt accgcaacca aaatctttgc
     1141 ttccgatgcg ttaactaacg gttctctggc taaaaacctg atgtttgctc aaaccaccaa
     1201 aggtgttctg gagacaggca tctaccgtgg tgtagtaagc atttatctgt ctcaggatat
     1261 ctaatccaga taacagaatt aacacgtaac aaagggaggg gtagccctcc cttattttaa
     1321 ctgttttagt atttgttatg gatttcagga ttgccatgga taaaaaatta ctggctcttt
     1381 tgatcctggc gagtctcagc ccggcagagg cgacattaac caaaattccc gcagggtttg
     1441 aggttattgc tcagggacag caggagtata tcgaggttta tttttcaggg aaaagtctcg
     1501 gtaaatatta tgcaatggtt aatcttgata ccgtaacatt tcttgatcca gcaagtctat
     1561 ataacaagct ggaactggat gtagacgatc agaaaatcgc gcatatagtg aaagaaaaat
     1621 tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg ttatgtacgt actgactcag
     1681 ggtgtggttt tctgaatacc gatacgctgg aaataatcta taatgatgaa gaaagttcgg
     1741 caacgttgtt tattaatccg caatggaatt cagctttcga tgcgaagtca ttatatttaa
     1801 atccagacaa aaatactgtt aatgctttta tacatcagca agacatcaat gttctggcac
     1861 aggatgatta ccaatcgttg tctattcagg gaaacggtgc gctgggaata acagaaaata
     1921 gttatattgg tgcacactgg aatttcaacg gttatgatgc agatgatgtc agtgacagta
     1981 atgctgacgt cagcgatctc tattatcgtt atgatttttt acgtcgttat tatgtgcagg
     2041 cgggtcgcat ggacaaccgc acactattta atgcacaagg cgggaacttt acctttaact
     2101 ttctgccact cggtgcaatt gatgggatgc gtatcgggtc gacactgagc tatttaaacc
     2161 aggcgcaaag ccagcaagga accccagtaa tggttctgct ttcgcgcaat tctcgtgttg
     2221 acgcttatcg taatgagcaa cttttgggat cgttttatct caatagtggt tcgcaattta
     2281 ttgataccag ttcctttccg ccaggcagct acagcgtggc attaaaagtc tacgaaaata
     2341 accaactcac ccgcaccgag cttgtgccgt ttaccaaaac cggcggtctg actgacggaa
     2401 atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca ggtttctgat gatgaaagtt
     2461 cagcttatca gctaggagta cgcctgccat tacatccgca atatgagctc tacgcagggc
     2521 tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa taactggacg gcagatttag
     2581 gcggggtggg gaatcttgca atcagcgcca gcgtgttccg taacgatgac ggcggcaaag
     2641 gtgatatgca acaggccaac tggagtaatc cgggatggcc gacgttgggc ttttatcgga
     2701 ccaactctga cggcgatgct tgtacaaccg acagcagaga gagctataac gccttaagct
     2761 gttatgaaag tatttccgcg acggtttcac agaattttgt cggctggaat atgatgctgg
     2821 gttatacccg cacacaaaat aacactgatg atagtttgcg ttgggataaa cagcagagct
     2881 ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat ctctgaaact gtacaactta
     2941 gcgcttcccg cgcttttgtg atgcgtgact ggattttgag tacgtcagtc ggtgttttcc
     3001 atcgtaatga caacggtggc gataacgacg acaacggctt gtacttatcg ttttcgttat
     3061 ctgacacgcc aacgatggat agcaataaca acagccattc aacaaatgtg tctacggatt
     3121 atcgttatag cgaacaggat ggcgatcaaa cgtcatggca gttatcgcat accttttata
     3181 acgattcatt cagccataaa gaacttggcg taaccgtcgg aggcctgaac accgatacca
     3241 taaacagcgc ggttaacggg cgttgggatg ggcaatacgg aaatgtctac gctaccgtat
     3301 ctgatagtta tgaccgtaag aatcatgatc atctttcggc ctttacgggc acttatagct
     3361 ctacgctggc tgtcagtcgc tatggcgtta atttgggagc cagtggtaca gacgatttgc
     3421 tgggtgcggt actggtggat gtgaaaggct tctctgaaca ggatgaagat agtcaggatc
     3481 tgcaactcga agcgcgggtg gcaggcagcc gaactttgca gcttggtcaa agtgacagtg
     3541 tgttgttccc ttatcctgga tttcagtctg gttttgttga ggttaacgac agtagccagg
     3601 gcaatcagca ggggacaaca aacatcatta acggtgcagg gaatcgtgaa ttaatgttgt
     3661 tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt taactacaac tatatcggtc
     3721 gtttattact gccggcagcg gtgaaaaaat tcccgattgt ggggctgaac agcgccatgt
     3781 tactggtagc tgaagatggc ggatttacac ttgaaattaa cggcagcgaa aaagagctct
     3841 atctgctttc cgggcagcaa ttccttaagt gtccactgag tgttgtaaag aaacgcgcca
     3901 gcattcgtta cagcggagat gttacttgta gtgtggtgac ttattcacaa ttaccggagt
     3961 cgattcaggt tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc
     4021 aaagggaggt tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac
     4081 gctcactggc gttcaggcag ctcctgacgt gacaagtgaa attacgtatg atttggcatc
     4141 tggtagagcg gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat
     4201 gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc
     4261 gacagtacaa atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa
     4321 aggatttaaa acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa
     4381 agggcttcag acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca
     4441 ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa
     4501 tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca
     4561 ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt
     4621 cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt
     4681 taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt
     4741 taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg
     4801 gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga
     4861 acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg
     4921 caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact
     4981 gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa
     5041 attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac
     5101 gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt ttctatataa
     5161 acgacgaagc cgtctgataa ggcggcttcg tcattacaac ttaagttaaa cgttaatgtt
     5221 gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat cgccggtttg ttctgcaaca
     5281 cggtccagat aaccagcgca cctaacaggt caaatactgc cagaactgcg aacagcgggc
     5341 tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc
     5401 atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag
     5461 aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca
     5521 gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca
     5581 cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct
     5641 ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca
     5701 tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg
     5761 ggatccacgc gttaaaagta ccccaggccg gttctgccag gaaacgcggc agcgcgatac
     5821 cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct
     5881 ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct
     5941 ggtcgcgtgg atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac
     6001 cggagatgat aaatgccatc tgccagctgt gcattacgat tgcccatacc accagcggcg
     6061 gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct
     6121 ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg
     6181 cggcaccgac tgcaccacga gcaatcgcca ggccacccca gctaccagcc agcgcggttg
     6241 caccacagaa cacggcccac agcacagcaa acattgcata accgattttc gtacccagca
     6301 catccagtac atagcccgct accggttgca tgaccgtata agcagcagaa taggctgcga
     6361 tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg
     6421 ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc aagcgtcacc agtgcgatca
     6481 tataccaacg taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc
     6541 aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga
     6601 aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta
     6661 ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta
     6721 tgatttgcga cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcaca
     6781 aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc
     6841 tgcagagaga tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca
     6901 cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag
     6961 caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc
     7021 ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt
     7081 ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac
     7141 ctgtatgaca tctggctgaa aggcgatcac tacaaatggc gcgctatgcg taccaacggt
     7201 gtggccgagc gtctgtgtac cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg
     7261 gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt
     7321 cgtccgtttg gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac
     7381 gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg
     7441 aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag
     7501 atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc
     7561 ttcaacatcg aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat
     7621 accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc
     7681 gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg
     7741 aacgaagcgg aactggacag cattctggcg cgccgtctgg ctggcgaacc cctgagcgag
     7801 cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt
     7861 cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc
     7921 aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat ggcggaagag
     7981 ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctctac
     8041 tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa
     8101 ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt
     8161 atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgctt tgtcggtatg
     8221 ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg
     8281 tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg
     8341 ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg
     8401 aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg
     8461 tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc
     8521 tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca
     8581 acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac
     8641 acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac
     8701 ctgattttca ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg
     8761 ctaacggcga tgtcggggtg cgtaatgagc tgtggatcct gccaaccgtg ggctgtgtca
     8821 acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta
     8881 ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca
     8941 tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg
     9001 tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata
     9061 tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg
     9121 ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggca
     9181 aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta
     9241 ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg
     9301 tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg
     9361 acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag tactttattg
     9421 cccatgacca gccgatctat gaaaacccat cgccggggaa caaagcgggc ggtatcacca
     9481 cgctggaaga taaatccctt ggctgtaccc agaaagcggg ttccagcgtc gtggttgacg
     9541 tgctgcgtta cggcgagcgt ctgaaaacgc cggggctgaa cttgttaagt gcgccgggta
     9601 acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta
     9661 ctggtcgtgg cacgccatac ggcggctttg taccgacggt gaaaatcgct accaacagtg
     9721 aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta
     9781 aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgtgttt gccaacggta
     9841 aacaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa
     9901 cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt
     9961 ttgcttttcc atacctgata aggatttatc atgaccgcgt attggctggc ccagggcgtg
    10021 ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc
    10081 aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc
    10141 tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg
    10201 cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag
    10261 ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac ctgggcgctg
    10321 ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg
    10381 attcacaact tctgggcggg gtcgataggc ggcacaatga ttgagggtag ttttctgctt
    10441 atgaatggct tgaatatcat tcgtttctgg cggatgcaga aaaggggaat tgatccgttt
    10501 aaagtagaga aaacccctcc cgccgtagac gaaaggggtt aaacaattaa ttacgcaggg
    10561 cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag
    10621 ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgatgata aagccgacgg
    10681 caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc agcgggatca
    10741 gcagcagaga accccccgcc acgccggatg cgccacaggc acacagagaa gccaccacgc
    10801 tcaacagcag cgccgtgggc agatcgaccg ggatacccag cgtattaacc gcagccagcg
    10861 tcaacacggt aatggtgatt gctgcgccag ccatattgat ggtggcaccc agcggaatag
    10921 aaacggaata ggtatcgcga tccagattca gcttttcaca cagtgccata ttcaccggaa
    10981 tgttcgccgc agagctgcgg gtgaagaagg catacacgcc gctttcgcgc aggcacagca
    11041 gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg ttaaccacca
    11101 gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg
    11161 tggagaaacc ggtggttgcc agggtagaag aaaccagacc aaaaatacca atcggtgcga
    11221 agcgaatgac cagtttcacc ataaaggtga cggcattcga catatcgttg accaggtttt
    11281 tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc aattgcccac accaggatcc
    11341 cgatgtagtt acctttcagc agcgcgtcga tggggttgga aaccatgctc attaccagcc
    11401 cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta ctggataaat
    11461 gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagcagca gagaaggtac
    11521 ccagcagata gaggaacaaa atagggcgga tattggtttt ctgcccgtgc tggtggttag
    11581 caatagatgc catcaccagc atcaacacca ggatgggggc aacggctttc agtgcgccga
    11641 cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc catgccagaa
    11701 gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga gccagacgcc
    11761 ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac
    11821 atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt gcaaggtcga
    11881 gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag agaggtcata
    11941 ttttgcaact taatggttac atttatttga cataaaaata aagccaacgt gcggcttcca
    12001 gactgatgac aacatcaaaa aaggactcgg tcagtcccct cgccccgttt ggggagaggg
    12061 ttagggtgag gggaacaggc cagcatcggt gcgaaaaatt accctcaccc cgaccctctc
    12121 cctggaaggg agagggagaa aacggtgctc atgttaatga cagggtacac ggcttaggca
    12181 gattaaaaat tatccacccc gctgcttatc atgccgataa ttcacccagg cgttgataat
    12241 aaacgtcatc accagaatgc caaacaccac gcccagcgag acggcgattg gaatatggta
    12301 gaagtcgaca atcagcatct tgataccgat aaacaccaga atcaccgcca ggccatattt
    12361 gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag
    12421 gatcgcaaac aggtttgagg tcagcacaat gaacgggtca gtggtcacgg cgaagatagc
    12481 cggaatgcta tccacggcga aaatcacgtc gctcaattcc accagaatca gtaccagcat
    12541 cagcggtgtg gcgtacagca atccattctt acgcacaaag aaatgctcgt tgtcgatggt
    12601 gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtctc caatgcctga
    12661 ttcatcttca tgggcgagcg ccatcttcac gccggtaaac agcaggaagg caccgaagat
    12721 atacaggatc cagtcgaact gcgaaattag ccagctgcca gtgaagatca tgatggtacg
    12781 cagaacaatc gccccgagca cgccatacac cagcacgcgg cgttgtaatg ccgccggaac
    12841 agagaaatag ctgaacaaca tcagccagac aaagacgtta tcgaccgcca gcgatttctc
    12901 aatcagataa cctgtgagaa aggccagtgc ctgtggatcg gcaacggcgc gaccttcggt
    12961 ttgcaccaga taccaccaga aagcggcgtt aaacagtaac gacagcgtca cccagaccag
    13021 cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa
    13081 caggtcgata gccagcataa tggcgacaac aacagcgaat ccgccccata gcaacggcgt
    13141 gccgacagta ttcatagaag ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc
    13201 agccgctgct ttttatgcat agacctcgcc ttccggcaag gtctcactta caacaaaaaa
    13261 ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc ggatgtggtt tttcacacat
    13321 cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa agtatgacag
    13381 accattcagc cagtcaatgg tctgttacat accttttaca ctgttttact ttggcaatta
    13441 tagttttacg ctatctgccg gaaaaatcac cccagtctgg cggcggatct cggtcagcag
    13501 tttggcggtg atgcgactga ccgccagccc cggatgatcc accagatgct catccaccag
    13561 ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct gggtgagatc
    13621 ctgcatttgg ctgccacgcg gcacgaagca cactttctgg cattcagaca gtttttcaat
    13681 caccagcgat cctgcttcgc cctgaatctc gctcgccagg acagaatcac tgacttttga
    13741 gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccgt gggcatctac
    13801 accgcttgcc agcaaactgg cggttgcctg cacgcttttc ggctcaccaa ataacgccac
    13861 cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga atgacggatt
    13921 aaaggtgttg ggattttcac cgtccaggta acgttgatag cgcgaggaat actggcaata
    13981 gttgaaaaag actttacgca atttgccgac tttcggcagc gcctggcgca gcaaatgaaa
    14041 gttcggcagg caggcggttt taaatgcctc aaacagcacc acctgatttt cccgcgcaca
    14101 ggcaatggcg gcacccactt ccgccagatt cgacgccagt ggtttctcgc aaatcacatg
    14161 aattttatgg ctaaggaaaa gttgtgtctg ggaaaaatgc agggcattcg ggctggcaat
    14221 atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgaggtaa acagatgctc
    14281 gacagaaaaa tcattggcga agtgctgagc ctgctcaagg ctgcgggaat atacggcggt
    14341 taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga tccagttcgt
    14401 accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat tctgtcgtca
    14461 atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt ttgccggaca
    14521 gccatgctca ctaaacggcc caatcatgac cgatggcccg agatatttcg gctggcgatg
    14581 gaggattttg gcgtcaatca cggcgctgac acgagcgagt ttctcccgca gttgagtata
    14641 aaaaccgtgc ttcatattga ggtcaggcgc gttaaaaccg atagcgtcga tgctgtactg
    14701 ttttgccagc cagatggcgc gttggttatg aaattcttgc gagataatgg tgatatggtt
    14761 ttcaccaaaa acctttttgg cacgcactac cgaatccagc gttgagaatc cggcatagtc
    14821 gcagaagatc actttcgccg gtacgccttt agcgatcaaa gcctgctgca tacctgaggc
    14881 ttcatcgtaa tttttacgtc cgttatcgcc acttaccagt agccatttca cttttccggc
    14941 gtggtacaac gctgctgcgg tatcaatacg tcgggtaaag tagcgattac ccggcctggc
    15001 ccccagcaat aagcccacgt tacgctccgg aactgcgttg acatcgctcc aggtcagctg
    15061 cttgctggcg ttgaccataa cgcgatcggc aataaaaatg gtcgctcctg ctataagcaa
    15121 caacaaacag ccaatcttaa gagtccggcg agaaaaacaa atacggagaa gaaggcgggg
    15181 ctgcatggtc gttatcctta acgaaagggg ggaggtcgat tttaggcgct aaagaaagaa
    15241 aagataacgc ctgacatcat tttcagattt tgtcctgccc aagcgacaac tgcgttagcc
    15301 acagccgggt gtcgaactca agctggtggt actgcggctc catatggcag cacaactggt
    15361 aaaacgcttt gttatgctct ttctccttca ggtgcgccag ttcgtgcacc acgatcatac
    15421 gtaaaaacgg ttccggcgcg ttgcgaaaca cagtagcgac gcggatctct gcttttgctt
    15481 tcagcttgcc gccctgcaca cgagaaacag cggtatgtaa cccgagcgcg ttttttagca
    15541 catggatctt attgtcatac atcactttat tgatcggcgg ggcattacgc agaaactgat
    15601 ttttcagatc ctgagtatat tgccagaggg ctttatcggt ggcgtagtcg tgtgttcccg
    15661 gatagcgttt tgccagcaca tcgcccagac gctgttcgtt aatcagcgtt cgcacctggg
    15721 aaagtagctg ctcgggataa ccctgaagat aagtaagatt gttcataacc gcccacgaaa
    15781 aagagaaaaa gggtatactc acgcaccctt ttcaggggca acgtcgaaat tttatcattc
    15841 aggagggccg atgagccact tagacaacgg tttccgttca ctgacactac aacgttttcc
    15901 ggcgacggat gacgttaacc cgctacaggc gtgggaagcg gcggatgaat atttgctgca
    15961 acagttggac gacacagaaa tccgcggccc ggtgttgatc ctgaatgatg cctttggtgc
    16021 gttaagctgc gcgctggcgg aacataagcc gtacagcatt ggcgactcat acatcagtga
    16081 actggcgacg cgcgagaatt tacgcctcaa cgggattgat gagtcgagcg tgaagtttct
    16141 cgacagcacc gccgactacc cgcaacagcc gggtgtggtg ctgatcaaag tgccgaaaac
    16201 actggcattg ctggaacagc aactgcgtgc gctgcgcaaa gtggtcacgt cggatacacg
    16261 tattattgcc ggtgccaaag cccgtgacat tcacacttcc acgctggagc tgttcgagaa
    16321 agtgctcggc ccgaccacca ctacgctggc atggaaaaaa gcgcgcctga ttaattgcac
    16381 tttcaatgaa ccgccgctgg ccgatgcgcc gcagaccgtt agctggaagc tggaaggtac
    16441 tgactggact atccacaacc atgcgaatgt cttctcccgc accgggctgg atattggcgc
    16501 acgcttcttt atgcaacatc tgccagagaa tctcgaaggt gagattgtcg atctcggctg
    16561 tggtaacggc gttattggcc tgacgctgct tgataaaaac ccgcaggcga aagtggtgtt
    16621 tgtcgatgaa tcgccgatgg cggtcgcttc cagccgaatg aatgttgaaa ccaacatgcc
    16681 agaggcgttg gatcgctgcg agtttatgat caacaacgcg ctctccggag tggagccttt
    16741 ccgctttaat gctgtgctct gcaacccgcc gtttcatcag caacatgcgc tgaccgataa
    16801 cgtcgcctgg gagatgttcc accacgcccg ccgctgcctg aaaatcaacg gcgaactgta
    16861 tatcgttgcc aaccgtcacc tggattactt ccataaactg aagaagattt tcggcaactg
    16921 caccaccatc gccacgaata ataaatttgt ggtgctgaaa acagtgaagc tggggcgtcg
    16981 tcggtaagat tgggagttac ctgccggatg cggcgttaac gccttatccg gcctacgttc
    17041 agcacgttac tcccaggccg gataagacgc ggcaagcgtc gcatccggca atgtctgccc
    17101 agttctggaa tcgcgcttcc ataaaacaaa aaatccctgt caccatcctc tacattctct
    17161 tgtttagcgt tgttcaacgt ttatgctaca tacgtgaagt tatgacgcat aaagaatacg
    17221 atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg ccgacatctt
    17281 gcaagcagga gaaaagctaa ctgcggtggc accttttctg gcgggtattc agaacgagga
    17341 acaatacgcc caggcgctgg aactggtaga tcatctgctg ctcaacgatc ctgaaaaccc
    17401 cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc ccgaatttgc
    17461 ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc gtacccttat
    17521 ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat ctatggtgtc
    17581 acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat tggcaacgcg
    17641 attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg cgttgcatcc
    17701 ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg caattgcccg
    17761 tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag ttttcccgct
    17821 atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga tcaccacatt
    17881 gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt catcgtcaat
    17941 cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcaggg tggtgcgatg
    18001 gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc gctggagcat
    18061 cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc caccagcctg
    18121 ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct ggctggtgga
    18181 ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac aaccgatgat
    18241 ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca tcgagataac tcaatacctt
    18301 cggatgatcg atcccgtcga taggcggaac gcgcggcacg atcccactgg cgaggatcgt
    18361 ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca gcgtcacgcc
    18421 cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg taaaactcct ctttgccggg
    18481 gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgggcatcaa acaatgttac
    18541 ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc ccgcaccgac
    18601 caccgccaga ttttttttct gcacggcagg aaggattggc attttggttt cgtggcaggc
    18661 gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga ggcaggcctg
    18721 attgcagcca atgcaggtgt tgatctcatc ggctcgcccc gattgcgctt ttgacagcag
    18781 ttccgcatca gcaagaaacg gtcgtgccat cgacaccata tcggcatcgc cgcgcgagag
    18841 aatgtcgtcg gcaacctgcg gatcgttaat ccggttggtg gtcaccagcg gcagcgaaac
    18901 gtggcctttc agtttgcgcg tgacccaagt aaatgcgccg cgcggcacgg gcgtggcaat
    18961 ggtcggaata cgtgcttcat gccagccaat gccggtgttg ataatggtcg cgcccgccgc
    19021 ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc ccgccgtctt cgaccaggtc
    19081 gagcatcgac agtcggtaga taataatgaa gtcgttgccg acgcgttcgc gcaccgcacg
    19141 cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact ggtcactacg
    19201 ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcggacc ccattacctc
    19261 tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag tgggcgaaat cgtcgatcag
    19321 ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg gggcctgcaa
    19381 tgcggaggga gcgaccagat gcggttggta gctgtagcgc ccggtatgca aaatttgcag
    19441 ggctattttg ccgccttcct gatgtaccgc ttcggtaatg gtgcgatggt gtgggatctg
    19501 gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat ctggtgcaat
    19561 gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa acgctgccag
    19621 ccgctcggca ccgtccgggt attcctccag cccggtgtgc attgagccca tcaacacgcg
    19681 gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt agctcataaa
    19741 attgtccagt atgtaaaata attgttatgt ggtcggatga gttctaattt agccgtcgcc
    19801 ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga atacgcccgg
    19861 tggtaacgcc ggatgcgacg cttgccgcgt tttatccggc ctgggggatt gcacatgtag
    19921 ggcggataag gcgttcacgc cgcatccgcc agtggcacgg tgcagatgcc ggatgcgacg
    19981 cttgtcgcgt tttatccggc caggaggatt gcacatgtag ggcggataag gcgtttacgc
    20041 cgcatccgcc agcagttgca aattattgct tacggaaaaa atcgttatac aacatataca
    20101 aatgcgcggc actccaggag aaatttggtg cgccttgctg tgcgccagtc agcgggttgt
    20161 aattctcctg aattgggcca tcggcggtta atcctttggc gtgacggaag aacgtatccg
    20221 ccagtttcag ggcatcatcg cgataaccgt aacgctccat ccctttcaga ccaaaccaga
    20281 actgatccac ccatacgcgc ccacgccagt agatatcagc gccaaaagcc ggattggtta
    20341 acgccgccgt tcccagcggg acaaaggtgt tgaactcttt aggatcgagc atcaccttca
    20401 ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg
    20461 gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt
    20521 caatacgtac gtcatagtag aactgggtag tcgggtcgaa catacaggtg ttgatgtagt
    20581 ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc ttctggctta ccgaggatgg
    20641 ttgccatctc cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact
    20701 cctgcaatag cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca
    20761 ccgtccagtc gctacgtttg ccgccattag cgacatattt atccaactgt tctttgtcga
    20821 taaaaccaaa gacggcggca tcatcacgcc ccgattccca cgacgcagcg acctgtgccg
    20881 gaatttccag actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag
    20941 actgcgtctc ttctttgttg cctttcttca ccgtaaacag catctcgccg ctctcagtgt
    21001 tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc gttgccgttg tgatcgcggt
    21061 tacgtaacca ccagtcgtga taggccacca gtttcgggta catctctgcc aaccaggctt
    21121 tatcctgggt gacgttgtac acttccatca ccgaccaggc ggcaaggctg ggtttggtat
    21181 tacgttcgtt ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca
    21241 ggtcggggac aaagcccaca tcctgcggac gcacgctatc gccaggctgg atctgccagg
    21301 agaagaccgc gcggatattc tctttggcga tgtcgggatt gaaatgcgcc atcgcaaacg
    21361 cctgcttcca ggtatcccac ggccaggtct gattgccgga gaaccagcgc ccggtcaccg
    21421 acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg
    21481 tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc
    21541 ctttcttcag gtattcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg
    21601 ccagaatatc gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcagatggg
    21661 aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta aagcgattac
    21721 cgttgatttc agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg
    21781 tcagcagatc ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg
    21841 cgctgatttt gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt
    21901 tcccttcttt cgcttccagt ttttccagca gttcgccgtc ccacaccaga tccagcggtt
    21961 tattgctggt gattttggtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca
    22021 tttcgacttg cacatctttt gctgtcagtt tttgcaccag cgcaccggga atactgtatg
    22081 cctccagcgt aaagtcgact ttcttgccgt cctgccagac ggtcaggcgg tcgaaattgc
    22141 tggccataaa gttgatgtac tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg
    22201 tgttagggcc gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa
    22261 aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca gtacggttaa
    22321 tcacgttttt ataattgtcg gcgttagcgg catgggcgga aaaacttatc agcagagcgc
    22381 aggttactgg cgttaaaata gtttttattt tcatgaaatt acagtcctta ttaccagtag
    22441 aaatattgca tcaggaagat ttggtcgctg ggatcgtgag aggcattttt catgtagaag
    22501 cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt cgtaccagat accaaactgc
    22561 atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg
    22621 gtggagagat aaacgccctt taacgcttca tacatggcgc tcaccatcat cccactttca
    22681 cttcccggta cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg agcggcaaag
    22741 ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc cgtcggtgat ttccagaccg
    22801 ttcatccagg tgatgccttc ggcaaggtca tactggatgt aaccgttcat cattgccgga
    22861 taggtgtatt tgtcgtcata gcgatcgtac ttgccaaaat gtagccaggc tttgctttcg
    22921 tcatgatgtc cggcaggtgt ggcggtgacg ctataacgaa agttgccgct taagttctgc
    22981 accttcagcg cggtcataat gtcgcgggta ttgggaatga cgtagcccag atcgggggtg
    23041 aagtcgcccc accattgcag gtcatccagc gaggaatcct gacgcagact gagcatgatt
    23101 tcggtgccat cattggtacg ataaccgcca tagaaacggt taattccgcc ctcgaaacca
    23161 ccccagctat ggtctggcac ccaggcattg ccgtcgtggt cggcctgcac ggtccagcct
    23221 tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct ccaggccacc ttcaatatag
    23281 gtaccgtcgc tgtatttatg tttattctca ccctctaaat ccatatatcc accaacccct
    23341 aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg taattgcagg tgtttttgtg
    23401 tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag aaaaggcgta gcaaaacggc
    23461 agagcaagta atgctctgca tggtgcagtg attaatttca ttgtgttaat ccatgagcta
    23521 aagaaaaagt acagcctttt atcgttgtaa ttataaaaat ataaaggcag aaactatttt
    23581 ccccctcccc atccaggaag agggaaaatg cctgctcatt ttttattagt ctgctcaatc
    23641 aacattgctg gctggcgtcg cttcaatatg gcgctcttca gccgttaatc cctttatata
    23701 tttactgtat ttccaccacg caaagccgag gaagataaca ataccgccga cgttataaaa
    23761 aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa gcgacaaacc ctacggcaaa
    23821 tatcgcaatc agcattgaaa caacgataat accggtgcgg cgggagccca tgcggaaatc
    23881 gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa gccagcatga tgaataacgg
    23941 cggaagcatg gacgctgcgg cagtcatatt aataatagta ttcatcagat cctgcacggt
    24001 attggaaccg agcatcggga taatcatcag cgggatgacg atcaggaact ggatccacgc
    24061 tgcgcgcgct ggaacgccgt tttcgttcag ttcgacggtt ttcttaccaa agatgccttc
    24121 cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc cacatcagca gggaaccgaa
    24181 catcgcggta aaggacacca gaccgacaaa gcgattcatc aacgcttccg gtagaccaaa
    24241 atacgccgcc atgccgtgga atacctgcac cgatccgccg gtaaatttca actctttgct
    24301 gctgacgaag acgttaatca gcaccgagga gacggaatac agtacgccga taaaaatccc
    24361 ggcgaggatg atcactttaa cgaacgattt cgaaccgcct ttgacgtcgt taacgtacac
    24421 agcgacggac tccgcgccac ctgcggccat aaagatccag gtggtaacgc cgaggaacgc
    24481 ccagttgaag ttcgggatca tcgcgtcaac ggtgatgggg tcagcaggct gtacgccgcc
    24541 aaccagcgcc gtacctgcca gtaaaatgta ggagagcgtt aacagcagca tcagcgttga
    24601 agtgacggag gtaattggcc ccaacatttt cgccccgttg gtggaaaccc aggtggagaa
    24661 ggcgaacagc accatactga taatggtggt ggcaaccggc gtcataatat attcgtagcc
    24721 gaggaaggca tacgaagcat aagcaataac gcgcggcaac agtgaggtga aaaagaacag
    24781 gtttacgaac cagtaggtat aggcagtaat aaatgcccaa cgtccgccca gcgaactttt
    24841 tacccacgcg tagacaccgg cttctgagtt tttatttaac gaaacaaatt ctgcgatgat
    24901 cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa aacatcggtg ccgaggccag
    24961 tccaagctca atattattat tgataacgtt gttaaagctg taaacggcgg caaaagtcag
    25021 cgagagcaag ccgaatttgc cgattgtatt acgtttggta tcagacataa cacatctccg
    25081 aaaatgtgta gggtagcggt ggcggcaaac tccttttgcc gccgtagttg ttatttgtta
    25141 tggaaataac catcttcgat ggtgactttg agaaccactt ttttgaccgc gttattgcag
    25201 ataaaccgat acgcttcatg gatatcgcaa ataacgattt gcccttcgtg gacctcaacg
    25261 gtttctccgc agccttttaa atattcacgg tcagtttcat cacgataata ttccactacc
    25321 tgtaatgtct ctttcggcgc atattcaatt ttttgctgcc cttgcaggta gtaatgcact
    25381 tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg cggagtctgt ctccacgcgg
    25441 taagtcaatg agtcaccgat ggagtgggcg acgccaggct gaatgttgtc gatattttca
    25501 atcgcttcaa cgcagcgttg ccacttcttg ccagaggcgt aaatctggcg gaactgttct
    25561 aagttatcga tgatcctcat tgcttattct cgctgtgcaa attcgtggaa aagaaccctg
    25621 cgccgaactc atacgacgcc aggctttgcg cggtagcttc tccgccagaa accggcagca
    25681 acgtaaagcc gtagctgaag tcacggaacc agacgcgcca ggagtccagc acctcgctgc
    25741 cccaggagtt ggagccgagg ccaagcagct ggtggtcgag gttcagggtg atgtcatcac
    25801 tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt ttcctgggta tagcgccagg
    25861 cgctgaagtt aattgggcgc tgcggaacca ccagcagacc gttgccgtgg cggttagtca
    25921 gtgccgtcca gcggacatgc tgacggttgc cgttgttctg cgggaaggga tagttctcga
    25981 acatggcatc gacggtgctg cgccagatat cgatgatgtt agcctgctgg ctgtcggcgt
    26041 agttttcgcc cggtccacga ccgtaatacg ccacctgatc gtattcgccg ttaatcccca
    26101 tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc gtaacgctcg ccggaaagcg
    26161 ccacgttaac ctggccatcg gcagcgatgc gccagatgta ggtgcagcgc atcccgaagt
    26221 caaacaccgg cggggcaata actgtgcggc tgatgatcaa cacttcacca tcgctctgtt
    26281 ctacggcaaa gccgcgcaga tgttcctgca tgatctgcaa atgattcggt tgccacagcc
    26341 cttcgtactc ctgcttgtgg ttgtcgatca ttggcttgaa gaagttgatc tttggctcgc
    26401 gggtcagcag cgactcgcca ttcacctgcc aggatgtcgg tttgccactc atttttgaga
    26461 aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa acgatcgtct tccagcgtca
    26521 gcggacgcgc attatttggt gcgaaaggca ctggttgcgc ggtgttttcc ttcagcggga
    26581 actgataagt ggcgatagaa tgcccggctt cgctgtagcg ggtgcgggaa tctttggtca
    26641 ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg cagcgtgatc tgcaaggggg
    26701 cttcgctgtt cggcgcaacg tcgcgcagtt taatctgctg cgtcgcgagc gtttcacctt
    26761 cgacgcgcac ctctgcgtgc agggtgtagt catcaagcgt ggtaaaccac agtttatttt
    26821 cgactttcag ctcgccgtga gtcagatcca gcgcgtggat ttttaccggc gcgataacct
    26881 gtttgtactc tttcaggccc ggtcccggcg tctgatcgga atagatcaaa ccatcaagac
    26941 agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac cagacgttgc
    27001 cgttgtcatc ctgcgcctgg atcccgtggt cgcaccattc ccagacataa tgtccctgaa
    27061 tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcctggt ccgttcccca
    27121 tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca ccaaactcat
    27181 tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca gcatcgcgat
    27241 cttcttcgta atgcaccagt cgcgtgtcat ccagcgcctt cgctgcgtgg tacatcgcgc
    27301 ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc gacggatggt
    27361 ttttctgcgc gtgaatatgg cgaacaatgc gctcgacgta gactttttcc cactgcggat
    27421 cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg acgtcggttt
    27481 ccgccatcac aaacaggccg tagatatcac acagttcgta aaaacgcgga tcgttcgggt
    27541 agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga tctttctcga
    27601 cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg ttgacgccgt
    27661 gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg atatcacgga
    27721 agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc agcgtcatga
    27781 ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg acagtaaaag
    27841 caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg ctgtgcacca
    27901 cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag gcggcgagat
    27961 tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca aagtcggtac
    28021 gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca tcgcggaaga
    28081 tccccgctga ccaccacata tcctggtctt ccacgtaggt agagtccgcc cactgcatca
    28141 cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca aactctgcgg
    28201 tcaggcgact gcccttgctg aaacccacat actgaccgtt aacatagact tcaaaatagg
    28261 tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg ctgagggtga
    28321 aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca tcgatgggga
    28381 acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc cacatggcgg
    28441 ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct ggtacttgca
    28501 gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac aggctgctgg
    28561 tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag tacgcacgcg
    28621 gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata gggcctcccg
    28681 gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct ggggagttta
    28741 gtaaaatttt tactaaaaag tagcaggtaa atgcatttac tttaactgga tcacgaaaat
    28801 aaacggcagc actgtccggc aaaaacgatt gccggacagt gtgtaagggg atttaacggg
    28861 tcgtgccgcg cagttttaat ttgctgggaa cgaagactaa cagcggcagc gcgcgaccat
    28921 cgcgggcttt ttcatacacc aggttaacgc cctgacttcc catcatctcg gaatggatgc
    28981 gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg gatatcgtta acgctgataa
    29041 gcgaaatatc ctgtgggatg ttcaggcctc gttcatgaat tgcccgcagt acgccgatag
    29101 caatggaatc ggaagcaaca aacagtgcct tcggatagtc ttcccgcgcc agcatttgtt
    29161 ttgccagttc ataacccgac gaactggaaa aaccgccgcg ccagatatcc tcttcgcgta
    29221 ccacttgttt cagtcggcca tattccgcaa aggcgacctc acgaatatcc gccttgccag
    29281 gctcatcttc accgccaata aaaccaatac gattaacgcc ctggttgata tagaagtcga
    29341 tgatttcttt actgatgcgt gccagatcga tatccaccgc atcgtaaccg ctgccgggtt
    29401 cgtgaaagtc gataaaacag atattgtcgg tcaacgcaca ggcggcggcg cgcagggcgg
    29461 gcgtgggttt gccgacaatt aaaataccgg tgacgttttt aatgtctggt aatccgctgt
    29521 gttcataaca gttggtgagc tcaatgccca gcttttcgca ctgagtttca atgccgtggc
    29581 ggatcgccag atagtaagga tcgttgatct ccagctcctg ttggtagctg tagatagcca
    29641 gaatatggtg ttggttgact gcacctgtct ggagtttacg ggcactactg gtcttgtact
    29701 ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc ttctttcaca ttcaatgtcg
    29761 gatcgtcatt taagaccctg gatactgtcg ccagggatac gccagcttcg attgcgatgt
    29821 cttttagtgt tgccatgttt acctttttta ctttccctga tgtgcgcccc cattgtagag
    29881 gaagtgctta tcgggtcacc tgttttcagt aaataaagcg cgtttctttg acgctgtaac
    29941 ggtaacacag cggctcacga atgtgatcac gcctgcattc tccattttta gcctacagga
    30001 ttgagaaaaa cttttgctat agtttagtaa aattttaccc gaagggagga aacaatggaa
    30061 accgtggctt acgctgattt tgcacgtctg gaaatgcgcg tcggaaagat tgtggaagtg
    30121 aaacgccatg aaaacgccga caagctgtac atcgtacagg ttgatgtggg ggaaaaaaca
    30181 ctgcaaaccg tgaccagcct ggtgccgtac tacagcgaag aagagctgat ggggaaaacg
    30241 gtggtggtct tatgcaatct gcaaaaagcg aaaatgcgtg gtgaaacgtc ggaatgcatg
    30301 ctgttgtgtg cggaaaccga tgacggcagt gaaagcgtgt tgttaacgcc ggagcggatg
    30361 atgcctgcgg gcgtacgcgt cgtataaaaa gatcggatgg cgacgtcgta tcgccatccg
    30421 atttgatatt acgcttcttc gacacttact cgcatggccg ccagcgcctt acgcgccgct
    30481 ttgatcacca gttcacactg ttcaatggtc agtgtcagtg gcggttcaat gcggatcgtt
    30541 ttggcgttat tgagcgttcc ggccaccagt acgcgctggc ggaacatctc gctggcaaag
    30601 ttatagccga tttcgttatc aacaaactca attgccatca acatcccttt accacgcgct
    30661 tcctgtacca gatcgggata ttcccgcgcc agttgacgga aaccgtccag caacatatcg
    30721 cctttttgct cagcctgagc cggtaagttc tgctccagca acacattgat ggtcgccagc
    30781 gccgccgcac aggccagcgg gttgccgcca aaggtggtgg tatgcaggaa tgggttgtcg
    30841 aacagaactg aaaacacctc ttcagtggcg atggtcgcgc caatcggcat cacgccgccg
    30901 ccgagcgctt tggcaaggca gaggatatcc ggctgtacgt tctcatgctc gcaggcgaac
    30961 atcttgcccg tgcgccccat gcccgtttgt acttcatcga ggatcatcag tgcgccgaac
    31021 tcatcgcata gcttacgtac ggcggtgaga tagcccggcg gcggcagaat tacgccacct
    31081 tcaccctgaa tcggttcgag gatcaccgca gccacatcat caccggtttt tttgcactcg
    31141 ttaagagccg tgcgcatggc ttcgatattg ccaaacggca catgacggaa gcccggcagc
    31201 agtggcataa acggtttgcg gaaggtcgat ttcgccgtgg ccgacagcgc gccaagtgat
    31261 ttaccgtgga acgcgccgct ggtggcaata aaagtaaact tgccgcgcgg tgactggtaa
    31321 gctttcgcca gcttcagcgc tgcttcaacg gactcggtgc cgctattaca gaagaagctg
    31381 tatttcagtt taccgggcgt tagcgcagca agggttttcg ccaacatcgc ccgtaacgga
    31441 tcgagcagct cctggctgtg cagcggttgt ttcgcaagtt gattctgtac ggcggaaacc
    31501 acaactggat tacggtgccc cacgttgaaa attccaaaac ctcccaggca gtcgataaac
    31561 tcctgtccct gggtgtcgac aagcgtattt aaacctcccg cttgccactc tacggctccg
    31621 taatccccgc cggcggtaac agatttgcga tactctaaaa accccggatt gacatgctct
    31681 ttgaagtatt caatcacctc tcggttaagt gctttcatct cctcatgatc cagcgttcgc
    31741 ttctcaatga gattcagggc gtgggcgctg cacgctaaag ccgatgcgct cgaaggtaac
    31801 ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat accgatttaa gtattgcagg
    31861 gattgcgcca ctccggctgc gatcgcgaaa atcgggataa acacaaattc ggttatcatt
    31921 tgtgcaatat ttaatgctat ctgttaacat ttgttgttat tttgcacggt ttttgtcggg
    31981 tatggatttg atgattgctg cgcaaatgca cgttttcagg gcaataattg cctgattttt
    32041 gggcaagata attaacgttt tcgtatagta acagagtgaa agtattttaa atcaaagaat
    32101 tagatttgtg ctttaatcgc aaattgcgat ctaaatcaaa ttaatcggtt aaagataacc
    32161 gcagcggggc cgacataaac tctgacaaga agttaacaac catataacct gcacaggacg
    32221 cgaacatgtc ttctcatccg tatgtcaccc agcaaaatac cccgctggcg gacgatacca
    32281 ctctgatgtc cactaccgat ctgcaaagct atatcactca tgctaatgac acttttgtgc
    32341 aggtgagcgg ctttaccttg caagagttac aagggcagcc gcacaacatg gtgcgtcacc
    32401 cggatatgcc aaaagcggcg tttgcggata tgtggttcac cctgaaaaaa ggggagccct
    32461 ggagcggcat cgtgaaaaat cgccgcaaaa atggtgacca ttattgggtg cgggccaatg
    32521 cggtaccgat ggtgcgcgag ggaaaaatca gtggctatat gtcgattcgt acccgggcga
    32581 cggatgaaga gattgcggcg gtggagccgc tgtacaaagc gctgaacgcc ggacgtacca
    32641 gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg gctgggtaaa ctgccttcat
    32701 taccgcttcg ctggcgggcg cgtggagtga tgaccctgat gtttatcttg ctggcggcca
    32761 tgctttggtt tgttgctgcc ccggtggtga cgtatttcct ctgtgtgtta gtggtattgt
    32821 tggcaagcgc ttgttttgaa tggcagattg tgcgcccgat agaaaatgtc gcccgtcagg
    32881 cactgaaggt ggcgaccgga gagcgtaata gtgttgagca cctgaatcgc agcgatgagc
    32941 tggggctgac attacgcgcg gtagggcagc ttggcctgat gtgccgttgg ctaattaacg
    33001 atgtctcaag ccaggtgtcc agtgtcagaa atagcagtga gacgctggcg aaaggcaccg
    33061 atgaactgaa cgaacatacc cagcagacag ttgataacgt tcagcaaacg gtggcgacca
    33121 tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac ggcgtcggct gccgataaac
    33181 tttctatcac cgccagtaat gcggcagtgc agggtgggga ggcgatgacc acggtgatca
    33241 agacaatgga cgatatcgcc gacagtaccc agcgcattgg caccattact tcgctgatta
    33301 acgatattgc gtttcagacc aatattctgg ccctgaatgc ggcggtggaa gcggcgcgtg
    33361 ccggcgaaca gggcaaaggt tttgcagtgg tggcagggga agtgcgtcat ttagccagcc
    33421 gcagcgctaa tgctgccaac gatattcgca agctgattga tgccagtgct gataaggtgc
    33481 aatccggttc gcagcaggta cacgccgccg gacggacgat ggaagatatt gtggcacagg
    33541 tgaaaaacgt cacccagttg atcgcccaga ttagccattc aacgctggaa caggccgatg
    33601 ggctttccag cctgacccgt gcagtggatg agcttaacct gatcacccag aaaaatgccg
    33661 agctggtgga agagagtgcg caggtgtcgg cgatggtgaa acaccgcgcc agccgactgg
    33721 aagacgcggt gacggtgctg cattaatcgg tatgccggat ccggcggttg gagcacaatg
    33781 cctgatgcga tgctggcgca tcttatcagg cctgcggggt gtgtagcggg tgtaggccgg
    33841 ataaggcgcg tcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg gtgcggcgat
    33901 taatccagtt gcgtaatatc aaaagcggcg cggtcgatca ccgcaatgat cttttttatt
    33961 tgtgcatcac tgatatcgct ctggttgacg tgtaaatcca gcaccgcttt aaaattatcc
    34021 agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc caacgcaacg cgctttgatg
    34081 cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca gccactgagc gccttgttcg
    34141 gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga tggtaatcag cgactgctcc
    34201 tgcagaaaat ccagcgttgg gtagatgacg cccgggcttg gggtgtaatt cccctgggtt
    34261 aggttctcaa tcgctttaat caattcgtaa ccgtggctgt catcgcgcga gagaatatcc
    34321 agaatcacca gacgcaattc accgtgacca aagaaacgtt gccgacgacc gccgccgcga
    34381 ccgtggcgac caccgcagca ttgaccgtgt tcatgctggt gaccgtgtcc gcagtgctcg
    34441 tgttctgact tctcaccttt gcagcagccc tcatggcgtg gctggccttc atgtttacaa
    34501 cacccttcgt gatgatggct catatcagcc tccttttgct tgattagata tatctaattt
    34561 atatctgatg tgagcattac tgcaagtaca aaaatgatgt ttaaacgtgg aagcacctca
    34621 catccaggcc ggataagacg ttcacgccgc atccggcagc attgctatgg cactgaatgc
    34681 cttcacatac ttcagatata tcagttctac aaatcgcttg catttatcat gattaacaat
    34741 cattatcatt tacgaaaatt atttagatat atctgattac tatcacgaag gcgataacaa
    34801 tgaataactc cccccgctac ccgcagcgcg ttcgcaatga tctgcgcttc cgtgaactga
    34861 ctgtgttacg cgttgagcgc atcagcgccg gttttcagcg cattgtcctc ggcggcgagg
    34921 cgctggacgg ttttacatcg cgcggctttg acgatcacag caaactcttc tttcctcaac
    34981 ctgacgctca ctttgtgccg ccaacggtaa cggaagaggg catcgtctgg ccggaaggac
    35041 cacgcccacc gtcgcgtgac tatacgccgc tgtatgacga actacgccat gaactggcga
    35101 tcgatttctt tattcacgac ggtggggtcg ccagcggctg ggcgatgcag gcgcaaccgg
    35161 gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa gattacgcgt
    35221 atcagctgta tgtctgcgat gaatccggaa tgcccgcact gcgccgccgc ctggaaacgt
    35281 tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct
    35341 gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca catgatgagc
    35401 aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac ttcatctgga
    35461 taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg gaacagtatg
    35521 acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata tataaaaaga
    35581 aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc tggacttgaa
    35641 ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg gccatggtag
    35701 cggattataa agtaactccg tgtcgcaatc cagccattac cgtgcgcctg ctgtttttat
    35761 aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg agtaaatatg
    35821 gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa ccctgggctt
    35881 tcatccgctg ggactggttt tccctttgct catccagcaa atcgcttctg gaaggtgata
    35941 tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca tctgctggat
    36001 tatcgttgtg gcgtcaccaa actggtagac cgtccaacgg tgcaagccaa tgaagtttca
    36061 aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga ttatcagccg
    36121 caggcgttgg cgattctggg caaacaagca tatgaacagg gattcagcca gcgcggtgca
    36181 cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct gccaaatccc
    36241 agcggtttaa gtcgcgtttc actagagaaa ctggttgaag cgtatcgcga gctggaccag
    36301 gcgctggtag tgcgtgggcg ataaaaaacg ccaccgaaaa ggtggcgttt gtgcaaaggg
    36361 actagtgccg ggtgcggcgt aacgcctgat ccggcctacc gattaatcgt ccaggaagct
    36421 acgcaacact tcagaacggc tcgggtgacg cagtttgcgc agcgccttcg cttcgatctg
    36481 acggatacgt tcgcgggtaa cgtcgaactg tttacccact tcttccagcg tgtggtcggt
    36541 gttcatatcg ataccgaaac gcatacgcag aactttcgct tcacgcgcgg tcaggccagc
    36601 cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc gcagaatcca gcggcagctc
    36661 gagggtggta tcctcgatga aatcccccag atgcgaatct tcatcatcac cgatcggcgt
    36721 ttccatggag attggctctt tggcgatctt cagcactttg cggatcttgt cttccggcat
    36781 cagcatacgt tcagccagtt cttccggcgt cggttcacgg cccatctctt gcagcatctg
    36841 gcgagaaata cggttgagtt tgttgatagt ctcaatcata tgcaccggaa tacggatggt
    36901 gcgcgcctga tccgcgatag aacgggtgat cgcctgacgg atccaccagg ttgcgtaggt
    36961 ggagaacttg taaccacggc ggtattcgaa tttatcaacc gctttcatca gaccgatgtt
    37021 gccttcctga atcaggtcaa ggaactgcaa gccacggttg gtgtatttct tggcgataga
    37081 aataaccaga cgtaagttcg cttcaaccat ctctttcttc gcacggcggg ctttcgcttc
    37141 accgatggac atacgacggt tgatatcttt aacctgctcg atggtcaggc cggtttcttc
    37201 ttcaatctgc tgcagtttct gcagggcgcg atgcacttct tcagagacat cgtgcagttt
    37261 ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac caggtatcgc tggtttcgtt
    37321 gccggtaaac agggtaatga agtttttctt cggcattttg cactgctcaa cgcagagctt
    37381 catgatcaga cgttcttgcg tacgaacgcg gtccatcatg acgcgcatgc tgttgaccag
    37441 gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat acttcagaca gtttcaggat
    37501 ctcttcctga gcggcagcgt gactgcgacc tttcgctttg atggtgtcac gcgttacaac
    37561 gtactgagcg cgtagttccg caaatttttc gcgagccagt tccgggtcga tgctgttgtc
    37621 atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca tcttcgtcat cgtccagatc
    37681 ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt gccagatctt cttctgcgtt
    37741 cgggtcaaca aagccggtga tcagatcgga cagacgcgct tcttctgctt caacacgatc
    37801 gtactgttcc agcagatagg tgatcgcttc cggatattca gcaacggagc attgaacctg
    37861 gttgatcccg tcttcaatac gcttagcgat gtcaatttcg ccttcgcggg tcaacagttc
    37921 aacggtgccc atttcacgca tgtacatgcg taccgggtca gtcgtgcgcc cgatttcaga
    37981 ttccacgctg gaaagcacct gcgcggcggc ttcggcagca tcttcgtccg cggtgttttc
    38041 agccagcatc agatcatcgg catccggtgc ttcttccatc acctgaatgc ccatgtcgtt
    38101 gatcatttgg atgatgtctt cgatctgatc tgaatcgacg atatcttccg gcagatggtc
    38161 attgacctcg gcataggtca gatagccttg ctccttacca cgggtgacaa gaagtttcag
    38221 ctgtgactgc gggttttgct ccataagacg gtatccacac ttatttcatg aggttggtgt
    38281 tgggcgattg acccgatgtc cttcagccgt taacgctgaa atccaacggt tgtttaccgc
    38341 caacgataat tacgagggcg tacttatata tttgccgctg cctctcagtg cggctgtcgg
    38401 gggcttcccg atcgctcttc ggcacttaag ccgttaaatc actttttcgc cagctcctgg
    38461 tttaatgtcc agagctccag gcgttcttcg ttgcttaaac catgcgtgcg ctcacgagcg
    38521 attaactctt cctggcgcag ttcaagcagc gaatcaaaca tatggttgag tgagtcggtg
    38581 aaggtttgct cagcaatatt cttatctgct atatcgtccc acatcgacag tttttcaagg
    38641 gtggcagcat tatttgtacc acgatagtgc tctaaaagtt gcccggtggt cagacctggc
    38701 tgggagagac aagtgttgac cagttctctg aataagccaa gtccagggag cttattttca
    38761 tccagattct caagcggcgg gaccaacgtc gctaattctg gattttgcac cagcaaccct
    38821 ataagtatac gcatggtcgt gcgttttagc tgcggaacag ggcgagaaac gccgctctct
    38881 gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa gtatgcctaa tttgttgcct
    38941 aattcctgac gaagatatat tcgcagcgtt tcgcccggca cttgcgatat caatggcagt
    39001 gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca gatcaacttg cggcatcagg
    39061 ctgttaaaca gaaatgcgga aagcggcatc gcctgctcca tccgcgcttc aaacgcttct
    39121 ttaccttctt ttcgtaccag cgtgtcaggg tcttcgccat ccggcaaaaa cataaagcgt
    39181 agctgacggc cgtctgtcat gtaaggcagc gccgtttcca gcgctcgcca tgcggcatcg
    39241 cggcctgcac ggtcgccgtc ataacagcaa atgacattgt tggtcgcgcg gaacaacagt
    39301 tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa cggcgtaatt aatgccgtat
    39361 tgcgccagcg ccaccacgtc catatagcct tcgaccacaa gcagacgatt gggttcagcg
    39421 ttatcctgct gcgcttcata aagaccgtaa agctggcggc ctttatggaa aatgtctgtt
    39481 tccggcgagt tcaggtattt gggggtatcg ttgcccagca cgcgcccgcc aaaaccaatc
    39541 acccgaccgc gtttatcgcg aatggggaac atcacccgct cgcggaaacg atcgtaactg
    39601 cgtccctgat cgttagtgac caacatcccc gcatcaatca atgactggcg attttctgga
    39661 ttgccgccaa accgcttcag gacgttgtcc cagccggggg gcgcaaaacc aatcgcaaag
    39721 cgagcgataa cctcgtggct taatccgcgt ttttccagat actggcgcgc agacgtggca
    39781 acaggttgtt gtaaagattg ttggtaaaac gtattcagac cgtccatcaa ctgataaagc
    39841 gtttgcctct gatggcgctc gatctggctg gggccgctgc ctgcttcaaa tggcacttca
    39901 agattgtgca ttgctgccag ctcttcgacc gtttcaacga actcgagctt gtcgtagttc
    39961 atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa agcagtggta aaactgtttc
    40021 tcaccgttaa cggtgaatga cggggttttc tcgttgtgga atggacaaca cgcgtggaaa
    40081 ttcttgccct gctttttcag cttcacacgg gcatcgatca gatcgacgat gtcagtgcgt
    40141 gccagcagat cattaatgaa tacgcgtggg attcgtccag ccataggccc cgatttttag
    40201 caattcataa acgaaaataa gccgcgcatt cctttcggaa gcacggcctt acaactacaa
    40261 ctcggtctga tcggagagca acgctctcgg ggaattagta cagacgagtg cggcgtgcgt
    40321 tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga agctttagcg cgcttacgtt
    40381 cggtagtcgg tttttcatag aactcacgac gacgaacttc cgccagaaca cctgcttttt
    40441 cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg ctcgttttca cgtactttaa
    40501 ttaccggcat gtgcctctca cctttgatta attcggtttg ccgctggcat caacgccagc
    40561 ttatttcaaa atggtgcgga attttactgc aattgctgct gctttgtaaa gcaccgcggc
    40621 cttttttgaa agggactttt ataagggtga ggagtataca cgaggctttc tccaggggcg
    40681 aacaaagttt tacatcaacc cgcattggtc ctacactgcg cggtaataaa gcgaggtaaa
    40741 acaagtcatg cgtgtactgg gtattgaaac ttcctgcgat gaaaccggca tcgccattta
    40801 cgacgatgaa aaaggtttgt tagccaacca attgtatagt caggtgaaat tgcacgctga
    40861 ctacggcggc gtcgtgcctg aactggcctc ccgcgatcac gtgcgcaaaa ccgtaccgtt
    40921 gatccaggcg gcgctaaagg agtctggttt aacggcaaaa gacattgatg ctgtggccta
    40981 taccgcaggc cctggattag tcggcgcact gctggttggc gcaaccgtgg ggcgttctct
    41041 ggcgtttgcc tggaacgtta cggcaatccc ggtacaccat atggaagggc atctgttagc
    41101 gccgatgctg gaagataacc cgccggaatt tccgtttgtc gcgctgctgg tgtccggcgg
    41161 tcatacgcag ttaatcagcg tgactggcat tggtcagtac gagctgctcg gcgagtctat
    41221 cgatgatgcc gccggtgaag cgtttgataa aaccgcgaag ctgctggggc tggattatcc
    41281 tggcggaccg ttactgtcga aaatggcggc tcagggtact gccgggcgct ttgttttccc
    41341 gcgtccgatg accgaccgtc cggggctgga tttcagcttt tctggtctga aaacctttgc
    41401 ggcgaacacg attcgtgaca acggcaccga cgaccagacg cgtgctgata tcgcccgcgc
    41461 ctttgaagat gcggtggtcg atacgttgat gattaagtgt aagcgagcgt tggatcagac
    41521 gggctttaag cgactggtca tggcgggcgg cgtgagtgct aaccgcacgt tacgggcgaa
    41581 gctggcggaa atgatgaaaa aacgccgcgg cgaagtgttc tacgcgcgtc cggaattttg
    41641 tactgataac ggcgcgatga tcgcctatgc cggaatggtg cggtttaaag caggcgcgac
    41701 ggcggatctc ggcgttagcg tgcgtccgcg ctggccactg gcagagttac cggctgcgta
    41761 aaactatccg gtgccggaga gcaatttccg gcaccgtcct cacttaaagc aacaccacgg
    41821 gcatccacag caagccggta atcaccagca acacgaggaa tatcagtcca aaaatcgccc
    41881 ccagtcgcca gtaatccacc gttggcagat aaccactacc gtagtaaatc ggactgggtc
    41941 cggtggcgta tggtgtaaga atgctcccca gaccaattgc cgcgccaacc atcaggcaga
    42001 acaccggcag cgggatttcc ggcatcgcca gcgcagcggc aatcatcatc ggtgcgaggg
    42061 cggaggtata cgccgtggcg ctggcgaaaa agtagcgcag cagataaaac accacaatca
    42121 acgccaccat caccatcgtt ggcgaataac cgcttaagct gcctgctaac agtttgccaa
    42181 accagctaat aaaaccagtg ttgttgagtc cggtagcgag ggtgataagc gaggccagcc
    42241 agaagaaaac gttccacgcg gctttattac tgacaatgtc gtcccaactg ataatgcgca
    42301 gaagcagcat cagtgccacc acgctgtaac cgaccatcgc ggcatcgata taatcaccgc
    42361 cgaaaatcca cagcaccagc gcgcctacca tcagccccag catccgtttt tcacgcgaac
    42421 agagcgggcc cattgcctgc agttccgtct ctgcccagcg cggcacctga tcgcctgact
    42481 tcagtaccgg cgggtacagc acataagcca gccagggaac cagcagcacc agcaaaatac
    42541 tgagcggcaa catcccgagg aaccagtcgc cccaactcag cgtggcgtga gatgcgcttt
    42601 tcatcagtcc aattaacagc aagtttggtg ccatcgccgt caagaaaatg gcgctggtca
    42661 cgcagtcggc aacaatcccc atccacatga tgtacgagcc aatgctgcgc gaactgctgt
    42721 cgtttggttg tgattgatag agcggtggca ggttacggat gatgggatag ataatccccg
    42781 caccacgcgc cgagttggac ggtgttacag gtgcgaggat cagctcggag aacatcaccg
    42841 catagccgag aaacagcgtg cgatgtccca tctttttcac cagaatcagc gcaatgcggc
    42901 gtcccagccc ggttttttca tagcctgtgc caaacataaa ggcggcgaaa atcagccaga
    42961 taaccgaatt agaaaaaccg gaaaccgccc acgagaggga ttttgcagtg aatttaaagc
    43021 ctggctgggc gagctgttcg gggctgaaca gcagccaggg agagagtatg gcgatgatgg
    43081 agatacccac catcgccacc acggcacccg gcacgggttc gaggatcagt ccaacgatca
    43141 cgccagtaaa aacggcaaag tagagccagg tatgactctc cagccctgcg ggaaccggaa
    43201 ttagagcaat aatggcgatg accgccagcg gcgcaaggta tcgccaccat tcagtggaag
    43261 gtttcatcgt gttatgcctg cgaaaaagga gagggggcga gcctctccgg gagggttatt
    43321 tgatgtaatg gacgtgctcg cagatctctt ccacgatggg atcgcggcgt tcggcgaaca
    43381 gctttttgtt ttcggctatc aggttgttgc cgtgggtatc aatagagaca atcagcgggc
    43441 cgaactcttt gacccggcag acccacagtg actccggcat cccgagttct gtccagtgca
    43501 cttcttcaat ctcttccacc tgggttgccg ccagcaccgc gcagcctgcc gggaaaatca
    43561 catgtagcgc cttgaatttc tggcagcctt cttcggtcag cggccccatt ccgcctttgc
    43621 caaccaccag tttcacgccg gtctgctcga taaattcacg ttcaaaactt tccatacgca
    43681 tactggttgt cgggccaacg gaaaccatct cccatttatc gccgtttttg cgcacgatgg
    43741 ggccagcgtg gaaaatcgct ttgccgttga gatcgtaagg gatcggacgt ttcagttcga
    43801 tcaagcggcg gtgacaaacg tcacggcagg tcaccagcgt accggtcagg tagatcacat
    43861 cgccgacgcg aatatcttgc agatcttcag ctttgatcgg ggttgtcagg atctttttca
    43921 taacgcgctc cgggtgtgag acagattttc aaaggtgaga tcggcatgaa ccagcagtgt
    43981 gccgcgacga tgcgcccagc agccggtaga gacagcaacg ccgatggttg acggatggcg
    44041 ggcggcagat tcgatatgca cgcccatcac tgaactgttg ccggtcagcc cttgtggacc
    44101 aatccccaga cggttgagtc cttcttccag gcgtagctcc agctctgccg cttttggatt
    44161 gggatggcgg ctgccaatcg gacgcaaaat ggctttacgc gagagtacgg cggcggtttc
    44221 caccgaggtg gcgatgccca cgcccaccag taccggtgga caggcgttta cggcgagggt
    44281 ggagatattt tcgaagacga atttcaccac gccttcgtag ccttctgacg gcattaacac
    44341 tttcgagcgg ccaggtagcg tgcagccgcc gcctgccatg taaacttcga tttctgcatc
    44401 gtcattgtcg gggatgatgt cccaggtgac ccacggtacg ccgctaccgg tatttttgcc
    44461 ggtgtttact tcgtcaaaaa tttctaccgc attgtgacgt agtggcgctt tgacggttgc
    44521 ctcttccacg gcttgtttga gtatgctttg cagctcgcca agcagtggga agcgggaacc
    44581 gactttaacg aagaacataa tctccccggt gtcctgacag gcaggacgat tcaggtcaat
    44641 cgctttttgc atgttgtcga acatcgtatg gtagataatt ttccccatcg acgacgtttc
    44701 ggcatccttt agctgtttta gtttatccac cacgtcatca ggcattcggg tagaaatcat
    44761 ggcggtaaag ttagcgacaa tctctgtcaa cttattcact gcctgttgct tattactttc
    44821 gctcatcatg gcaactccag tttttgtatt tgtctggaaa aattcaccgc cacaaactac
    44881 cactaaaaat gtggttttat attttttgcc accgtgaata tgtaattaac agttcgtgaa
    44941 tcaacgcgaa aataagaatg aatattgcgc cagagcaggg aatggcaatg ttttgcgaaa
    45001 tacactgcgc ttatcacaac tgcggataat taacgatgct gaatagctgg cctttagcca
    45061 aagacttgca ggtactggta gaaatcgtcc acagcgggag ttttagcgcc gcagcggcga
    45121 cgcttgggca gacgcctgca tttgtcacca agcggatcca aatccttgaa aatacgctgg
    45181 caacaacgct gctgaaccgc tccgctcgcg gcgtggcgct gactgaaagt ggccaacgtt
    45241 gttacgaaca tgcgctggaa atcctcaccc aatatcagcg actggtcgat gacgtcacgc
    45301 aaatcaaaac gcgcccggag gggatgattc gtattggctg tagttttggt tttgggcgca
    45361 gccatattgc gccagccatt accgaactta tgcgcaatta tcctgagtta caggtgcatt
    45421 ttgaattgtt cgatcggcaa attgatttag tgcaggataa tattgatctg gatattcgta
    45481 ttaatgacga aattcctgat tattatattg cgcatctgtt aacgaaaaat aaaagaatat
    45541 tatgcgcagc acctgagtat ctgcaaaaat atccgcagcc acaatcatta caggaattaa
    45601 gtcgtcatga ctgtctggtg accaaagagc gcgatatgac ccacggaata tgggagttgg
    45661 ggaacggtca ggagaaaaaa tcggtgaagg ttagcgggca tctttcctcc aatagcggcg
    45721 agatagtgtt gcaatgggcg ctggagggca aagggattat gctgcgttcc gagtgggatg
    45781 tgttgccgtt tctggagagt ggcaaactgg tgcaagtatt gccggagtat gcacaaagcg
    45841 ccaatatctg ggctgtttac cgggagccgc tctatcgcag catgaaatta cgtgtctgcg
    45901 tggaatttct ggcggcatgg tgccagcaac ggctgggcaa gcccgatgaa ggctatcagg
    45961 tcatgtagat ccaccagaaa tcactcggga tccttttcgc gctttctttt gaatttcgtc
    46021 cagatttttg tctcctgacg acgccacaga cgctggatgt tgtcatgatg acgcagcagg
    46081 atcaggcaag agagcatcga aaccgggaag gtgaattgtg gcttaaacca ccatacataa
    46141 aacggagcaa tcagtgcact gacaatcgct cccagcgacg agtatccgct caatagcacg
    46201 gtcagtaacc aggttcccgc cattactccg gtgagatccc agccaatggg tgcgatggca
    46261 ccaaaagcgg tagcaacgcc ttttcctcct ttaaatccga agaaaacggg ccagatgtgt
    46321 ccaagacagg cggcgatggc aattaagcct agccagaagg ggctgacacc taattcatac
    46381 gcgccccaga cgggcaacat tcctttcaga acgtcgaaaa tcagtactgc tacggctgct
    46441 cccttgccac cgatacgtaa cacattggtt gcgcctggat tgccggagcc gctggttcgc
    46501 ggatcgggca gcccacacaa gcggcaaacc agaatggcac tggaaatgga gccgcagagg
    46561 tacgcgatga ggatcattcc aggcgcgatt gcactcataa gctgttccgt tttgaaaatt
    46621 cgtgttcaac gatgaatctg tggataatac gcacatttcg ccggaagtgg tatccggtta
    46681 gccaaaaagc aggcaggacg tgatggatat tgtatttata gagcaacttt cggtaatcac
    46741 cactattggt gtttacgact gggaacagac catcgaacag aagttagtgt tcgatatcga
    46801 aatggcatgg gataaccgta aagcggcgaa aagtgatgat gtggcggatt gcctcagtta
    46861 cgctgacatt gcagaaacgg tggtcagcca cgtcgagggg gcgcgttttg cgctggtgga
    46921 acgcgtggct gaagaggtgg cggagctgct gttagcacgc ttcaactcgc cgtgggtgcg
    46981 tatcaaactc agcaagccag gcgcagtggc gcgggcggcg aatgttggcg taatcattga
    47041 gcgtggcaat aatctgaaag aaaataatta attttacagc tgttaaacca aacggttata
    47101 acctggtctt actgcagtag tccggacaag cggtacattt taataattta ggggtttatt
    47161 gatgagcgat atgcactcgc tgctgatagc ggcaatattg ggtgtggtcg aaggattgac
    47221 agaatttctg ccggtatcca gcacgggcca tatgattatt gtcggtcact tgttggggtt
    47281 cgagggcgac acggcgaaaa cctttgaagt tgtgatccag ttaggatcaa ttctggcggt
    47341 agtagtgatg ttctggcggc gtctgtttgg cctgattggc atccactttg gccgcccgtt
    47401 gcagcacgaa ggagaaagca aaggtcgttt aacgctgatc cacattttgc tggggatgat
    47461 tccggcggtg gtactggggc tgttgttcca cgacacgatt aagtcattgt ttaacccgat
    47521 aaatgtgatg tatgcactgg tcgttggcgg tttgttgctg attgccgccg aatgcctgaa
    47581 gccgaaagag ccgcgtgcgc cgggtcttga tgatatgacc tatcgtcagg cgtttatgat
    47641 tggctgtttc cagtgtctgg cgctgtggcc gggtttctcc cgttccgggg ccaccatttc
    47701 aggtgggatg ctgatgggcg tgagccgtta cgctgcttcc gagttctcgt tcctgctggc
    47761 ggtaccgatg atgatgggcg caacggcgct cgatctctac aaaagctggg gcttcctgac
    47821 aaccggcgat atcccgatgt ttgccgttgg gtttatcacc gcttttgtgg tggcgctgat
    47881 agcgattaaa accttcctgc aattgattaa gcgcatttcg tttatcccgt tcgccattta
    47941 tcgctttatt gtggcggctg cggtgtacgt cgtgttcttt tgattgagaa aaggttcggg
    48001 agcaggcctg gttttctccc tcgctctgta gggagagggc cggggtgggg gtgattgttc
    48061 gcaccggtgc tggtctgttc ccctcaccct aaccctctcc ccgaaggggc gaggggatgg
    48121 ttcgtgttaa cgtttagttt tatactcagt caggctttgg gcaacgttgt tccttccaac
    48181 tggctaccgc cgcaatccgc cgtcgggtca gctcctcgcg aatctccaca cctttaaatc
    48241 ccgcttcaac gaccgctttt gtcggcactg actgcgccac ttcccaggct tcgcgcaacc
    48301 aacggccttg cgggtagtcc gctgattcaa aaccggttct gccgcgtacg tcagcctcgc
    48361 tggtcagcgc cagttgctcg acacgctgcg gtttacgcca ggcatcgatg gaatcaaata
    48421 atttgacgat ggtttttggg ttcagcattg ggaaggtgtg gatgagatcg tgaaactcag
    48481 ccaccagtct ggctaaatcg cgaatttcat ttggcacgcg cagacgctgg catagttgtt
    48541 ccactaactt aacacccgcc gggccatgcc catgatgacg cggccaaagt tctggcggcg
    48601 tcagtccttt accgagatcg tggcataaag ttgcgaaacg gacatcaacc tgcggactca
    48661 gcatcgccgc cattgagagc gtcattaagg tatgaatacc cgtgtcgatt tccggatgcc
    48721 acctggcagg ggccggaacg ccaaacaggg cgtcaatttc cgggaataaa acgcgaagtg
    48781 caccgcaatc gcgcagtacc tggaagaaca cctggggatt gcgggtggtg agggcgcttt
    48841 ccgtctcttt ccatacccgt tcaggcgtca ggtgttccag ttcacccgca tgggtcatct
    48901 cgcgcatcag cgtcagagtt tcatcggcaa tacgaaaacc gaggtgggca taacgcgcag
    48961 caaaacgcgc cacgcgcaga acgcgtaacg gatcttcgcc aaaagcgggg gaaacatggc
    49021 gcaacagacg attttgcaga tcgcccagac cgttgtacgg gtcgataatc tcgccgttat
    49081 cgtcctgagc cagcgcatta atggtcagat cgcgacgctt aagatcatct tccagcgtga
    49141 catccggtgc ggcatagcaa gtaaaaccgg tgtaaccgga accggatttc cgttcggtac
    49201 gtgccagcgc atactcttca tgcgtttgcg gatgcagaaa cacaggaaaa tcgcggccta
    49261 cctgctggta gcccgcgtcg agcatctcct gtggcgtact gccgaccacc acccaatctc
    49321 tgtctttgac cggtagccct aacaatgcat cccgaacagc accaccgacc agataaatct
    49381 tcacgccatc atcccttcgt caaatacaca attcctaata ataagacagt gcaacgaaga
    49441 aggcgattta gttcatccag cgatctttgc gtttgcgcgt tgggatcagg tgtggcagta
    49501 ccagaccgag cagcaagcca agccccagca cgccaccgcc atacataaac cattgcatga
    49561 tgatggtgcg ctgtttgtca tccagctgta cgctggcggc atcgaccttt ttctgcgcga
    49621 caatcagctc gtttttcaat ttctgatttt cttcttttaa tccgttgatc acgctgtcgc
    49681 tctgcgccac tttttgctgc atttctgcgg tgcgctgatt ccaggtgtta tcgatattgg
    49741 tgagtttatc ggtcagggtt ttgacctgat tttccagatc tggcacacgg gagcgcaggc
    49801 ttggctcagt gctaagttgt ttcaacggga tccaggcggt acggccagag ctgtctttca
    49861 cctgggcata attggtgttg gcgtcagttt gtaataaggt cacttcctcg ccggcgttaa
    49921 ccgtgcccac gaggcgataa tgatctcccg gaccgctacg gacccaggta ttcagttcat
    49981 cagaaacata gcgcgtttct tcagcgtgtg agacggcagt ggcgctaagt gcgagtaaag
    50041 ttaatccgat caggcgtaat tttggcatca ggctgtcgtt attgtcatgg aaagtggaac
    50101 gatagtagtg gcatcagtgt cgctacgcaa agcattcgac atcaatcgga atcctctgtg
    50161 tcaggtcttg ccactgcttc aaacttttac gcccgtcaaa ttgcccgttg catggcaatt
    50221 tggcgcaaaa tactatctac tgacaaaaaa gatcgcatta agttcgccgt tcatcgtcac
    50281 ttatgtgaca taaccgataa gtaaaggcca tggctcagga aatcgaatta aagtttattg
    50341 ttaatcacag tgccgttgag gcgttgcgtg accatctcaa tacgctgggc ggcgagcacc
    50401 atgaccccgt gcagttgctg aatatttact acgaaacgcc ggataactgg ctgcgtgggc
    50461 acgatatggg cttacgtatt cgtggcgaaa acggtcgcta tgagatgacc atgaaagttg
    50521 caggaagagt gacaggcggc ttacaccagc gcccggaata taacgtggcg ttgagcgaac
    50581 cgacgctcga cctggcgcag ttaccgacgg aagtctggcc gaacggcgaa ttgcccgccg
    50641 atctcgcttc ccgcgtacaa ccgctgttca gcaccgattt ttatcgcgaa aaatggctgg
    50701 tggcggtcga tgacagccga attgaaatcg ctctcgatca gggggaggtg aaagcggggg
    50761 aatttgctga acctatctgt gagctggaac tggaactgct tagcggcgac acgcgcgcgg
    50821 tgctgaaact ggcgaaccaa ctggtatcgc aaaccggatt acgccagggc agcctgagca
    50881 aagcggcgcg tggctatcat ctggcgcagg gcaatccggc gcgtgaaatc aaaccgacca
    50941 ccattttgca tgttgcggca aaagccgatg tggaacaggg gctggaagcg gcgctcgagc
    51001 tggcgttagc gcaatggcag tatcatgaag aactgtgggt acgcggcaac gatgcggcga
    51061 aagaacaggt gctggcagcc attggtctgg tccgtcatac actgatgctg ttcggtggta
    51121 ttgtgccgcg caaagcgagc actcacttac gtgatttgct gactcaatgc gaagcgacca
    51181 tcgcttctgc ggtttctgcc gtgacggcgg tctactctac caaaacggca atggcgaagc
    51241 tggcgttgac cgaatggctg gtaagcaaag cctggcagcc gtttttagat gccaaagcgc
    51301 agagtaaaat gagcgattcc ttcaaacgct ttgccgatat ccatctttcc cgccatgccg
    51361 ctgaactgaa aagcgttttc tgccagccgt tgggcgatcg ctaccgtgac cagttgccac
    51421 gtctgacgcg tgatattgac tcaatactgt tgctggcggg ttactatgat cctgtcgtcg
    51481 cgcaagcctg gctggagaac tggcaggggc tgcgtcacgc tattgcaacc gggcaacgca
    51541 ttgaaattga acatttccgt aatgaggcaa acaatcagga accgttctgg ttgcacagcg
    51601 gaaaacgtta atcaggcaag gataaaatcg cttatgaagc cgctctcttc accgttacag
    51661 cagtactggc agaccgttgt tgagcggctg ccagagcctt tagccgagaa atcacttagc
    51721 gcacaggcga agtcagtact tactttcagt gattttgtgc aggacagcgt gattgcgcat
    51781 ccagagtggc tgacggaact ggaaagccaa tcgccgcagg ccgacgaatg gcagcattac
    51841 gcggcatggt tgcaggaggc gctcagcaat gtgagtgacg aagccgggtt aatgcgcgag
    51901 ctgcggctat tccggcggcg cattatggtg cgcatcgcct gggcgcaaac gctggctttg
    51961 gttactgaag agagcatatt gcagcagctc agccatctgg cggagacgct gattgtcgcc
    52021 gcgcgtgact ggctgtatga cgcttgctgc cgcgagtggg gaacgccgtg caatgcgcag
    52081 ggcgaagcgc aaccgctgct gattttaggc atgggtaagc tgggcggtgg ggaactgaat
    52141 ttctcctcag atatcgatct gatttttgcc tggccggaac atggttgtac gcagggcgga
    52201 cgccgggaac tggataacgc gcagtttttt acccgcatgg ggcagcggct gattaaagtg
    52261 ttggatcaac caacgcagga tggcttcgtc tatcgcgtgg atatgcggct gcgtccgttt
    52321 ggcgaaagtg gcccgctggt gctgagcttt gccgcgttgg aggattatta ccaggagcag
    52381 gggcgcgact gggagcgtta cgcgatggtc aaggcgcgga ttatgggcga tagcgaaggc
    52441 gtctatgcta acgagttgcg tgcgatgctg cgcccgtttg ttttccgtcg ttacatcgat
    52501 ttcagcgtga ttcagtcgct gcgcaacatg aaagggatga ttgcccgtga agtgcgtcga
    52561 cgtggtttga ccgacaatat caaactcggc gcaggcggca ttcgcgaaat tgaatttatc
    52621 gttcaggtgt tccagctcat tcgcggtggg cgcgaaccgt cgctgcagtc gcgctcttta
    52681 ctgccaacgc tcagcgtcat tgccgcgctg catctgcttt ctgaaaacga tgctgaacaa
    52741 ttgcgagtgg cgtatctgtt cctgcggcgt ctggaaaacc tgctgcaaag cattaacgac
    52801 gaacaaaccc agacgcttcc ttctgatgag cttaaccgtg cgcggctggc gtgggcgatg
    52861 gactttgctg actggccgca actgaccggg gcgctgaccg cacatatgac caatgtgcgc
    52921 cgggtgttta atgaattgat tggcgacgac gaaagtgaaa ctcaggaaga gtcgctgtcg
    52981 gaacagtggc gtgagctgtg gcaggatgcg ttgcaggaag atgacactac gccagtgctg
    53041 gcgcatctta gcgaggatga tcgcaaacag gtgctaacgc tgattgccga tttccgcaaa
    53101 gagctggata agcgcaccat cgggccgcga ggacgtcagg tgctcgacca tctgatgccg
    53161 catctgctaa gtgatgtctg tgcgcgtgaa gacgctgccg ttacgctgtc gcgcattacc
    53221 gccttgctgg tggggattgt tacccgcacc acctatttag aattgctcag tgaattcccc
    53281 gcggcgctta aacatttgat ttctctgtgt gccgcgtcgc cgatgattgc cagccaactg
    53341 gcgcgttatc cattattgct ggatgaattg ctcgatccaa acacccttta ccagccgacg
    53401 gcgaccgatg cctaccgcga tgagttgcgc cagtatttgc tgcgcgtgcc ggaagatgac
    53461 gaagagcaac agcttgaggc gctgcgtcag ttcaaacagg cgcagctgtt acgcatcgcc
    53521 gcagcggata tcgctggtac gctaccggtg atgaaagtga gcgatcactt aacctggctg
    53581 gcagaggcta tgatagatgc cgtcgttcag caggcgtggg ttcaaatggt ggcccgctac
    53641 ggtaagccaa atcacctcaa cgatcgcgaa ggccgtggtt ttgcggtggt cggctacggc
    53701 aagctgggcg gctgggagtt aggctacagt tccgatcttg acctgatctt cctccatgat
    53761 tgcccaatgg atgcgatgac tgacggcgag cgggaaatcg acgggcgaca gttttatctg
    53821 cgtctggcgc aacgcattat gcatctgttc agtacgcgta cctcttccgg cattttgtat
    53881 gaagtggatg cacggctacg cccgtccggg gccgcgggaa tgctggtgac atccgcagaa
    53941 gcatttgccg attatcagaa aaacgaggcc tggacgtggg aacatcaggc gctggtgcgt
    54001 gcgcgggtag tgtacggcga tccgcagctc accgcgcact ttgacgcggt gcgtcgcgag
    54061 attatgacgc tgccgcgtga aggtaaaact ctgcaaacgg aagtgcggga aatgcgcgag
    54121 aaaatgcgcg ctcatctcgg caataaacat cgcgatcgct ttgatatcaa agctgatgaa
    54181 ggcggaatta ccgatatcga atttattacc caatatctgg tgttgcgcta cgctcatgaa
    54241 aaaccgaagt taacgcgctg gtcagacaac gtgcgcattc tggaactact ggcgcaaaac
    54301 gacattatgg aagagcagga agcgatggcg ctgacccgtg cttatactac gctgcgcgat
    54361 gaacttcatc atctggcatt acaggaattg ccgggccatg tgccggagga ttgcttcacc
    54421 gcagagcgtg aactggtgcg ggcaagctgg cagaagtggc tggtggaaga atgaagtatg
    54481 gtattatcgc gcgcaaattt tgaatctctc aggagacagg aatgaaagta acgctgccag
    54541 agtttgaacg tgcaggagtg atggtggttg gtgatgtgat gctggatcgt tactggtacg
    54601 gtcccaccag ccgtatctcg ccggaagcgc cggtgcccgt ggttaaagtc aataccatcg
    54661 aagaacgtcc gggcggcgcg gctaacgtgg caatgaacat cgcttctctc ggtgccaatg
    54721 ctcgcctggt cgggctgacg ggcattgacg atgcggcgcg tgcgctgagt aaatctctgg
    54781 ccgacgtcaa cgtcaaatgc gacttcgttt ctgtaccgac gcatccgact atcaccaaat
    54841 tacgggtact ttcccgcaac caacagctga tccgcctgga ttttgaagaa ggtttcgaag
    54901 gtgttgatcc gcagccgctg cacgagcgga ttaatcaggc gctgagttcg attggcgcgc
    54961 tggtgctttc tgactacgcc aaaggtgcgc tggcaagcgt acagcagatg atccaactgg
    55021 cgcgtaaagc gggcgttccg gtgctgattg atccaaaagg taccgatttt gagcgctacc
    55081 gcggcgctac gctgttaacg ccgaatctct cggaatttga agctgttgtc ggtaaatgta
    55141 agaccgaaga agagattgtt gagcgcggca tgaaactgat tgccgattac gaactctcgg
    55201 ctctgttagt gacccgttcc gaacagggta tgtcgctgct gcaaccgggt aaagcgccgc
    55261 tgcatatgcc aacccaggcg caggaagtgt atgacgttac cggtgcgggc gacacggtga
    55321 ttggcgtcct ggcggcaacg ctggcagcgg gtaattcgct ggaagaagcc tgcttctttg
    55381 ccaatgcggc ggctggcgtg gtggtcggca aactgggaac ctccacggtt tcgccgatcg
    55441 agctggaaaa tgcagtgcgt gggcgcgcag ataccggctt tggtgtgatg accgaagagg
    55501 aactgaagct tgctgtagcg gcagcgcgta aacgcggcga aaaggtggta atgaccaacg
    55561 gtgtctttga catcctgcac gccgggcacg tctcctatct ggcaaatgct cgcaaactgg
    55621 gtgaccgtct gattgtcgct gtgaacagcg atgcctccac caaacggctg aaaggggatt
    55681 cccgcccggt aaacccactc gagcagcgta tgattgtgct gggcgcactg gtagcggtcg
    55741 actgggtagt gtcgtttgaa gaagacacgc cgcagcgtct gatcgccggg atcctgccag
    55801 atctgctggt gaaaggcggc gactataaac cagaagaaat tgccgggagt aaagaagtct
    55861 gggccaacgg cggcgaagtg ctggtgctca acttcgaaga cggttgctcg accactaaca
    55921 tcatcaagaa gatccaacag gataaaaaag gctaaccgga aagcggttca cagatctcgc
    55981 ttcgtagcag gcaggcctat tgacgctgtt gttttttcaa cctaaagtaa aggaacaagg
    56041 gtaagggagg atttctcccc cctctgatca gttgttagta agtcgggaaa cttaacagta
    56101 acaacacaac cagtatgatg acgaacttca tcataaccct ttccttctat aaggcccctt
    56161 cctcgggagg ggctttcccg tttcagcccc ttgctgactc cccgattgtt gatgagcgcc
    56221 ggaccccgcg caacgctttc cctgaagcag ttttgccttc cccgagcggt cacgcaaaac
    56281 gctgcaacgt cctgagcatt tgcacctttt agcggaacgc agatataaaa aatccccggc
    56341 tttttgggcc agggatatgt gttaatgaaa ccttcttgac gctgattttt tttcaaccta
    56401 aagtaaagga acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct
    56461 gcgaaactta cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc
    56521 ttctgtaagg ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg
    56581 attgttgatg agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg
    56641 agcggtcacg caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat
    56701 ataaaaaatc cccggctttt tgtgccaggg acgttcatag ctgaaaattt attctacgtt
    56761 ttcggcgacg ggagttgttg aggttaaggg tgaagtcaat gccgccagtg agccgcctga
    56821 aacgccaatt tcattgagca aggagtcaat cagcggtgcc tgtgtgcggt aagagagagc
    56881 ggctgataat gcttgttccg ccaggtttcc gccccccaca ttacccgtgt ttgcatcacc
    56941 cgcagcgctg ccacgattca gaccatcgac ctgaataatc ttgataccgt caatagattt
    57001 catcggctca acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa
    57061 tttaaggctg gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc
    57121 ttctgcttcc gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc
    57181 ctgcatctct gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt
    57241 ttctgcatcc tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt
    57301 ctggcgagtg gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc
    57361 acgcgcttct gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg
    57421 gttggcgatt tcggtgacct gctgttgttc gatctcttta atacgaactt cacgttcagc
    57481 ctcaaccttt cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg
    57541 ttcagccaga attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat
    57601 acgtgcattc tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa
    57661 cgcttcttgt tgttcaatct ccaacttgcg cgaaagtgca tcacggtttt tctcacgcac
    57721 cgcaacttct acatcctgtt caacttcgtt acgttcgcga cgacggcgct ccgtctcctg
    57781 agtcagtttg gttaaacctt cggcgtcaaa ggcattgttc ggattgaaat gttctttcga
    57841 ggtctggtta aagttggtaa gtgaaacgct ttccagctca agaccgtttt tcgacaggtc
    57901 ttctgcgaca gtattttgta ctccctgcac gaagttctcg cgggtatcct gtaactcatg
    57961 catggtcatt tgtgcagctg ttgcacggag ggcatcgaca aatttatctt caaccaacat
    58021 acgtaagtct tctggtgata gcgtgcgttg ccccaacgtc tgcgccgcag tggcgatgcc
    58081 ttctacagac ggttttaccc gtacaaagaa agcgactact acatcgacac gcatacgatc
    58141 tttcgtaatc aggctatcaa tggttgagcg gctgacttcc agcttcagag tattcatatt
    58201 gatggggatt atttcatgaa agatcggcat cacgattgcg ccaccgctca tcaccacttt
    58261 ttgcccacct aaaccagtac gaacaaaagc ttgctctgcc gaagcgcgac gatagagcct
    58321 ggcgaaaata attccaataa taaacagaat gcatacggca ataatcgcgg taaacat
//
LOCUS       DAZFWP020000010        53249 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFWP020000010 DAZFWP020000000
VERSION     DAZFWP020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 53249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.010"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            299..385
                     /gene="leuL"
                     /locus_tag="ACHK0Z_RS02850"
                     /old_locus_tag="pgaptmp_000570"
     CDS             299..385
                     /gene="leuL"
                     /locus_tag="ACHK0Z_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU4500640.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            478..2049
                     /gene="leuA"
                     /locus_tag="ACHK0Z_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             478..2049
                     /gene="leuA"
                     /locus_tag="ACHK0Z_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU4500641.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2049..3140
                     /gene="leuB"
                     /locus_tag="ACHK0Z_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             2049..3140
                     /gene="leuB"
                     /locus_tag="ACHK0Z_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU4500642.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3143..4543
                     /gene="leuC"
                     /locus_tag="ACHK0Z_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             3143..4543
                     /gene="leuC"
                     /locus_tag="ACHK0Z_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU4500643.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4554..5159
                     /gene="leuD"
                     /locus_tag="ACHK0Z_RS02870"
                     /old_locus_tag="pgaptmp_000574"
     CDS             4554..5159
                     /gene="leuD"
                     /locus_tag="ACHK0Z_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU4500644.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5208..6386)
                     /gene="setA"
                     /locus_tag="ACHK0Z_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             complement(5208..6386)
                     /gene="setA"
                     /locus_tag="ACHK0Z_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGU4500645.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6488..6619)
                     /gene="sgrT"
                     /locus_tag="ACHK0Z_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             complement(6488..6619)
                     /gene="sgrT"
                     /locus_tag="ACHK0Z_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU4500646.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6708..8363
                     /gene="sgrR"
                     /locus_tag="ACHK0Z_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             6708..8363
                     /gene="sgrR"
                     /locus_tag="ACHK0Z_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU4500647.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8397..8496
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8527..9510
                     /gene="thiB"
                     /locus_tag="ACHK0Z_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             8527..9510
                     /gene="thiB"
                     /locus_tag="ACHK0Z_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU4500648.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9486..11096
                     /gene="thiP"
                     /locus_tag="ACHK0Z_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             9486..11096
                     /gene="thiP"
                     /locus_tag="ACHK0Z_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU4500649.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11080..11778
                     /gene="thiQ"
                     /locus_tag="ACHK0Z_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             11080..11778
                     /gene="thiQ"
                     /locus_tag="ACHK0Z_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU4500650.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11892..12656)
                     /gene="yabI"
                     /locus_tag="ACHK0Z_RS02905"
                     /old_locus_tag="pgaptmp_000581"
     CDS             complement(11892..12656)
                     /gene="yabI"
                     /locus_tag="ACHK0Z_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGU4500651.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQNGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12742..13620)
                     /gene="araC"
                     /locus_tag="ACHK0Z_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             complement(12742..13620)
                     /gene="araC"
                     /locus_tag="ACHK0Z_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU4500652.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13959..15659
                     /gene="araB"
                     /locus_tag="ACHK0Z_RS02915"
                     /old_locus_tag="pgaptmp_000583"
     CDS             13959..15659
                     /gene="araB"
                     /locus_tag="ACHK0Z_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU4500653.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPLLNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15670..17172
                     /gene="araA"
                     /locus_tag="ACHK0Z_RS02920"
                     /old_locus_tag="pgaptmp_000584"
     CDS             15670..17172
                     /gene="araA"
                     /locus_tag="ACHK0Z_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU4500654.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17372..18067
                     /gene="araD"
                     /locus_tag="ACHK0Z_RS02925"
                     /old_locus_tag="pgaptmp_000585"
     CDS             17372..18067
                     /gene="araD"
                     /locus_tag="ACHK0Z_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4500655.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18142..20493
                     /gene="polB"
                     /locus_tag="ACHK0Z_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             18142..20493
                     /gene="polB"
                     /locus_tag="ACHK0Z_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU4500656.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20658..23564
                     /gene="rapA"
                     /locus_tag="ACHK0Z_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             20658..23564
                     /gene="rapA"
                     /locus_tag="ACHK0Z_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU4500657.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23576..24235
                     /gene="rluA"
                     /locus_tag="ACHK0Z_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             23576..24235
                     /gene="rluA"
                     /locus_tag="ACHK0Z_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU4500658.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24352..25167)
                     /gene="djlA"
                     /locus_tag="ACHK0Z_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             complement(24352..25167)
                     /gene="djlA"
                     /locus_tag="ACHK0Z_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU4500659.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25422..27776
                     /gene="lptD"
                     /locus_tag="ACHK0Z_RS02950"
                     /old_locus_tag="pgaptmp_000590"
     CDS             25422..27776
                     /gene="lptD"
                     /locus_tag="ACHK0Z_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU4500660.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27829..29115
                     /gene="surA"
                     /locus_tag="ACHK0Z_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             27829..29115
                     /gene="surA"
                     /locus_tag="ACHK0Z_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU4500661.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29115..30104
                     /gene="pdxA"
                     /locus_tag="ACHK0Z_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             29115..30104
                     /gene="pdxA"
                     /locus_tag="ACHK0Z_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU4500662.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30101..30922
                     /gene="rsmA"
                     /locus_tag="ACHK0Z_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             30101..30922
                     /gene="rsmA"
                     /locus_tag="ACHK0Z_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU4500663.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30925..31302
                     /gene="apaG"
                     /locus_tag="ACHK0Z_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             30925..31302
                     /gene="apaG"
                     /locus_tag="ACHK0Z_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU4500664.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31309..32151
                     /gene="apaH"
                     /locus_tag="ACHK0Z_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             31309..32151
                     /gene="apaH"
                     /locus_tag="ACHK0Z_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU4500665.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32229..32708)
                     /gene="folA"
                     /locus_tag="ACHK0Z_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             complement(32229..32708)
                     /gene="folA"
                     /locus_tag="ACHK0Z_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU4500666.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32900..34762)
                     /gene="kefC"
                     /locus_tag="ACHK0Z_RS02985"
                     /old_locus_tag="pgaptmp_000597"
     CDS             complement(32900..34762)
                     /gene="kefC"
                     /locus_tag="ACHK0Z_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU4500667.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGFIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34755..35285)
                     /gene="kefF"
                     /locus_tag="ACHK0Z_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             complement(34755..35285)
                     /gene="kefF"
                     /locus_tag="ACHK0Z_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU4500668.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35393..36724)
                     /gene="yaaU"
                     /locus_tag="ACHK0Z_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             complement(35393..36724)
                     /gene="yaaU"
                     /locus_tag="ACHK0Z_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4500669.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36783..37070)
                     /gene="fixX"
                     /locus_tag="ACHK0Z_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             complement(36783..37070)
                     /gene="fixX"
                     /locus_tag="ACHK0Z_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU4500670.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37067..38353)
                     /gene="fixC"
                     /locus_tag="ACHK0Z_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     CDS             complement(37067..38353)
                     /gene="fixC"
                     /locus_tag="ACHK0Z_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4500671.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38404..39345)
                     /gene="fixB"
                     /locus_tag="ACHK0Z_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     CDS             complement(38404..39345)
                     /gene="fixB"
                     /locus_tag="ACHK0Z_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4500672.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39360..40130)
                     /gene="fixA"
                     /locus_tag="ACHK0Z_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     CDS             complement(39360..40130)
                     /gene="fixA"
                     /locus_tag="ACHK0Z_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU4500673.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40604..42118
                     /gene="caiT"
                     /locus_tag="ACHK0Z_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             40604..42118
                     /gene="caiT"
                     /locus_tag="ACHK0Z_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU4500674.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGCNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42149..43291
                     /gene="caiA"
                     /locus_tag="ACHK0Z_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             42149..43291
                     /gene="caiA"
                     /locus_tag="ACHK0Z_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU4500675.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43420..44637
                     /gene="caiB"
                     /locus_tag="ACHK0Z_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             43420..44637
                     /gene="caiB"
                     /locus_tag="ACHK0Z_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU4500676.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44711..46251
                     /gene="caiC"
                     /locus_tag="ACHK0Z_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /pseudo
     CDS             44711..46251
                     /gene="caiC"
                     /locus_tag="ACHK0Z_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
     gene            46360..47145
                     /gene="caiD"
                     /locus_tag="ACHK0Z_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             46360..47145
                     /gene="caiD"
                     /locus_tag="ACHK0Z_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU4500677.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            47151..47741
                     /gene="caiE"
                     /locus_tag="ACHK0Z_RS03045"
                     /old_locus_tag="pgaptmp_000609"
     CDS             47151..47741
                     /gene="caiE"
                     /locus_tag="ACHK0Z_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU4500678.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47827..48222)
                     /gene="caiF"
                     /locus_tag="ACHK0Z_RS03050"
                     /old_locus_tag="pgaptmp_000610"
     CDS             complement(47827..48222)
                     /gene="caiF"
                     /locus_tag="ACHK0Z_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU4500679.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48483..51704)
                     /gene="carB"
                     /locus_tag="ACHK0Z_RS03055"
                     /old_locus_tag="pgaptmp_000611"
     CDS             complement(48483..51704)
                     /gene="carB"
                     /locus_tag="ACHK0Z_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU4500680.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51722..52870)
                     /gene="carA"
                     /locus_tag="ACHK0Z_RS03060"
                     /old_locus_tag="pgaptmp_000612"
     CDS             complement(51722..52870)
                     /gene="carA"
                     /locus_tag="ACHK0Z_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU4500681.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        12775 a        14441 c        13991 g        12042 t
ORIGIN      
        1 atcgcaaaac atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt
       61 gtcccctttt tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga
      121 aaagcgtcgg tagttaagca gaaattaata tcgcttactt taaccaccgc agcacaatta
      181 gctaatttta cgggtgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac
      241 atccgttttt gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat
      301 gactcacatc gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag
      361 acgagtgagc ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt
      421 gcgcgggttt ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg
      481 agccagcaag tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca
      541 agcttgagtg tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac
      601 gtgatggaag tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc
      661 gcccgccagg ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc
      721 gacgtggcgg ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct
      781 acctccccaa tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc
      841 gctatctata tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa
      901 gatgccgggc gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc
      961 ggtgccacca ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc
     1021 ggaatcatca gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta
     1081 catacccacg acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt
     1141 gcacgccagg tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg
     1201 gaagaagtca tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt
     1261 aatcaccagg aaatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc
     1321 ccggcaaaca aagccattgt tggcagcggc gcattcgcac actcctccgg tatccaccag
     1381 gatggcgtgc tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg
     1441 aaccaaatcc agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg
     1501 gacgagatgg ggtataaaga aagtgaatat aatttagaca atttgtacga cgccttcctg
     1561 aagctggcgg acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc
     1621 ggtaagcagc aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc
     1681 tctaacgata tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa
     1741 gccgccaacg gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac
     1801 tataacgtcg aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg
     1861 ggtcaggtgg atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc
     1921 accgatattg tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt
     1981 gccgcagaag tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa
     2041 accgtgtgat gtcgaagaat taccatattg ccgtattgcc gggggacggt attggtccgg
     2101 aagtgatgac ccaggcgctg aaagtgctgg atgccgtgcg caaccgcttt gcgatgcgca
     2161 tcaccaccag ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc
     2221 cgcctgcgac ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg
     2281 gtccgaaatg ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgttgcctt
     2341 tgcgtaagca cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg
     2401 aagcattctg tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc
     2461 gcgaactgac cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat
     2521 atgaaaaagc gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca
     2581 tcgcgtttga atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg
     2641 tgctgcaatc ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg
     2701 atgtcgaact ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat
     2761 cacagtttga cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg
     2821 caatgatcac tggctcgatg gggatgttgc cttccgccag cctgaacgag caaggttttg
     2881 gtctgtatga accggcaggc ggctcagcac cagatatcgc aggcaaaaac atcgccaacc
     2941 cgattgcgca aattctgtcg ctggcactgc tgctgcgcta cagcctggat gccgatgatg
     3001 cggcttgcgc cattgaacgc gccattaacc gcgcattaga agaaggcatt cgcaccgggg
     3061 atttagcccg tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct
     3121 atgtggcaga aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca
     3181 cgtagtgtac gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca
     3241 tgaagtgacc tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca
     3301 gccgggcaaa accttcgcca ctatggatca caacgtctct acccagacga aagacattaa
     3361 tgcctgtggt gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt
     3421 tggcgtcgaa ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc
     3481 ggaacagggc gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac
     3541 ccacggcgcg tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact
     3601 ggcaacgcaa accctgaaac agggccgcgc aaaaaccatg aaaattgaag tccagggcaa
     3661 agccgcgccg ggcattaccg caaaagatat cgtgctggca attatcggta aaaccggtag
     3721 cgcaggcggc accgggcatg tggtggagtt ttgcggcgaa gcaatccgtg atttaagcat
     3781 ggaaggtcgt atgaccctgt gcaatatggc aatcgaaatg ggcgcaaaag ccggtctggt
     3841 tgcaccggac gaaaccacct ttaactatgt caaaggccgt ttgcatgcgc cgaaaggcaa
     3901 agatttcgac gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt
     3961 cgataccgtt gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa
     4021 tccaggccag gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc
     4081 ggttgaacgc gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggcattcc
     4141 gctgaccgaa gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga
     4201 agatttacgc gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc
     4261 actggtggtt cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa
     4321 aatctttatt gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat
     4381 gaacaacgac cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga
     4441 aggccgccag gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc
     4501 tgctgtgacc ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag
     4561 agaaatttat caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg
     4621 atgcaatcat cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc
     4681 tgtttaacga ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc
     4741 tgaacttccc gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg
     4801 gctcctcgcg tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg
     4861 cgccgagttt tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga
     4921 aattaagcga tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc
     4981 atttcgacgt ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta
     5041 ccatcgatgc cttccgccgc cactgcatga tgaacggtct ggacagtatt gggcttacct
     5101 tgcagcacga cgacgccatt gccgcttatg aagcaaaaca acctgcgttt atgaattaat
     5161 ccccttgccc ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta
     5221 acctttgcgg ttaaaaataa tgcgacaaca gaaataaccg caattaccca gtagacagca
     5281 aagtgccccc aactttgtgc aattgctccc tgaataacgc cagccagaat taccccggta
     5341 gaaatactgt tagtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga
     5401 aaccatagca tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc
     5461 gtcatcaacg ccatacggct atgaaagaaa atcaatccgg tgtaaaacag tactcctgcc
     5521 gccactgcta tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga
     5581 atcattgctg gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct
     5641 ggcaatccta actcgctact gatccacaac ggcatatcaa taatgtacat ggtgttgcag
     5701 gtccacatta acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagccacct
     5761 tgcattgata aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat
     5821 gcaatcaata ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat caccgtaaag
     5881 ccgtaattca acgccagcat aaaggccaac ggtggaccga taacccatgc cagagaaagc
     5941 tgcgcacgca tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcatattcc
     6001 cgcgccagag caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca
     6061 caggtgataa gcgtcagata atgacgatta aatgcaaaca atagcgcatt gccgatagcc
     6121 atcaaacagc aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc
     6181 aaccagaggc ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag
     6241 aaaggttgcg cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc
     6301 ccggccaccc ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg
     6361 cgagccatcg tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa
     6421 accagcaggt ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc
     6481 aggcaagtca actttcagaa ttgcggtcat cccactgcat cagttcttca agcatggtta
     6541 atcgttgtgg gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt
     6601 gctgataaaa ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa
     6661 tatagggaaa ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc
     6721 gtctgcaaca acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa
     6781 cgctcaacga actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca
     6841 acaccatgca ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct
     6901 cgcgtctgac attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc
     6961 tggagcagga tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc
     7021 aaatgctggt ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc
     7081 tctactatcg tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc
     7141 atattgcccg gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg
     7201 aagcagacat cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc
     7261 gtccaggagt ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt
     7321 taaaacgaat caatacgctg ccgctctatt cgcatattgc ggacattgtg tcgccgacgc
     7381 cctggacgct ggatatccat ctcacgcaac cggaccgctg gttaccgtta ctgctgggac
     7441 aagttccggc gatgatcctg ccgcgcgaat gggaaaccct cagtaacttt gccagccatc
     7501 ccatcggcac cggtccgtat gcggtgatcc gcaacaccac caatcaactg aaaattcagg
     7561 cattcgatga cttcttcggt taccgggcat taatcgatga agttaacgtc tgggttctgc
     7621 cggaaattgc cgacgagcca gctggaggtc tgatgctaaa aggtccacag ggtgaggaaa
     7681 aagagattga aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc
     7741 atcgcggggc gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc
     7801 tggtctattt cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc
     7861 cccgttggca ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca
     7921 tcctgacctt ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga
     7981 ttctggcaag tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg
     8041 aaggagagat cgagagcgat atctggctta acagcgccaa ctttacgctg ccgctggatt
     8101 tttcgctgtt cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact
     8161 ggcaagccga cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc
     8221 aactggtcgc cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac
     8281 aacgcagtat gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt
     8341 ggtttgcgcc gccggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt
     8401 tctcaacggg gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg
     8461 ataacgccgg cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg
     8521 tgcaaagtgt taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct
     8581 aaacccgttc tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca
     8641 aagatcaaaa aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa
     8701 gatggcgttt cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg
     8761 gtgctggggc tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa
     8821 agcggtgtgg cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc
     8881 ccgtttgatt acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg
     8941 caaagcctga aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg
     9001 cgcaccagta cgccggggct gggcctgctg ttgtggatgc aaaaagtcta tggcgatgac
     9061 gccccacaag cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc
     9121 gaagcctacg gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct
     9181 ccggcttatc acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa
     9241 ggtcactatc tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg
     9301 gcgcaaaaat tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt
     9361 aactggatgt atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa
     9421 ccggccacca cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt
     9481 agcgaatggc aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg
     9541 ccgccacgct ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc
     9601 cgcagggtaa ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct
     9661 ccttctggca ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc
     9721 gcgccctcta tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga
     9781 ccttgatcct cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg
     9841 gctggctggc atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc
     9901 tgcaaggcat tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac
     9961 tccaggcact ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc
    10021 gcggctggca ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg
    10081 ttgccgcgct tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg
    10141 gcggtccgca ggcaactact atcgagctgg ctatttatca ggcgctgagt tacgactacg
    10201 atcctgcccg cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc
    10261 tgttgagtca gcgactaagt aaggccattg cgcccggtac cacgctgctg caaggctggc
    10321 gcgatccgga cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc
    10381 tgctgctgtt gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc
    10441 cggaagtgct ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc
    10501 tggcggcagg tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac
    10561 tgcgggcgcg gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc
    10621 tcgccatgcc ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg
    10681 gcctgccaca atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt
    10741 atgcgctgaa agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt
    10801 gtcagtcgct gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga
    10861 aacgtccact ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg
    10921 tagtggcgtt gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa
    10981 ttggctccta tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt
    11041 ttctgctgtt taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc
    11101 acctggcttt atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag
    11161 gtggcaatcc tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt
    11221 tttctgacgc cagccagcgg ttcgctgact atcgacagcg ttgatcacac cacgacgccg
    11281 ccgtcacgcc gtccggtatc gatgctgttt caggagaaca acctgttcag ccacctgacg
    11341 gtcgcgcaga acatcgggct ggggcttaat ccgggattga agctgaacgc ggcgcagcag
    11401 gagaaaatgc acgctatcgc ccgccagatg gggattgata atttaatggc gcggttaccg
    11461 ggcgagcttt ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa
    11521 cagccgattt tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag
    11581 atgctgacgc tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg
    11641 cacagtgtgg aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc
    11701 atcgcctggc agggtaagac caatgagttg ttgagcggta aggcgagtgc ttcggcacta
    11761 ttggggatta cgggttagta tgcactgtcg gatgcggcgt gaacgcctta tccgacctac
    11821 gggtagtgcg ttttgtaggc ctgataagac gcggtcagcg tcgcatcagg caacccgcac
    11881 aagactatct cctaaccccc aaccacttta cgcaaaatat cgatatacac cggcatcaac
    11941 gggtggcgaa ttaacaccac cagcgccacc acgccgatgg cagaaattaa cggcgtcagc
    12001 cacaacaaac ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc
    12061 cataaccgcc agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac
    12121 catttaaact cgccgttctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc
    12181 ccaggcagga agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat
    12241 tttgccaccg gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca
    12301 acaaagcgac cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta
    12361 tcgagtaatg ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc
    12421 cagcccagcc agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccag
    12481 gcgtgccaga aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc
    12541 gtaccgggta gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc
    12601 gccatcaatg aatacacggt ggattgggta ataaagtgtt ccagcaatgc ttgcataatg
    12661 tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc
    12721 aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc
    12781 acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata ctcgcgagaa
    12841 atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtagt
    12901 gctcaaaagc agcttcgcct gactaatgcg ttggtcctcg cgccagctta agacgctaat
    12961 ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaggcaaa catgctgtgc
    13021 gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc
    13081 gcgtacacga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag
    13141 taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc
    13201 ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg
    13261 aaagaaaccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg
    13321 cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg
    13381 atgaatctct cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc
    13441 ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc
    13501 cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc
    13561 caccagatgg gcgttaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat
    13621 acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg
    13681 ccgtcactgc gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa
    13741 aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta
    13801 taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat
    13861 agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac
    13921 tgtttctcca tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg
    13981 attttggcag tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg
    14041 ccaccagcgt agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata
    14101 accagttccg tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg
    14161 tgcttgcaga gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta
    14221 ccggctcgac gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt
    14281 ttgccgaaaa cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag
    14341 cggaagagat tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg
    14401 gcggtattta ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca
    14461 gcgccgtggc gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc
    14521 tttccggtac cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat
    14581 ctctgtggca cgaaagctgg ggcggcctgc cgccagccag tttctttgat gagctggacc
    14641 cgctcctcaa tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc
    14701 cggtgggcac cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga
    14761 tttccggcgg cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg
    14821 cactggtaaa agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg
    14881 ttggcgagcg ggcagtgaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat
    14941 ttatcggtct ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg
    15001 tactcggctg gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca
    15061 acgccagcca gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc
    15121 tggatcacct gccggtggtg ctcgactggt ttaacggccg ccgcacaccg aacgctaacc
    15181 aacgcctgaa aggggtgatt accgatctta acctcgctac cgacgctccg ctgctgttcg
    15241 gcggtttgat tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc
    15301 aggggatcgc cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca
    15361 ttatgcaggc ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt
    15421 gctgtgcgct cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc
    15481 cctcagccca gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg tgcagcgaac
    15541 aggcacaacg ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac
    15601 aacactatct tccaacttcc gccccggcac aggctgccca ggccgttccg actctataag
    15661 gacacgataa tgacgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat
    15721 ctgtatggcc cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt cgttaatgcg
    15781 ctgaatacgg aagcgaaact gccctgcaaa ctggtgttga aaccgctggg caccacgccg
    15841 gatgaaatca ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg
    15901 gtgtggctgc acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac
    15961 aaaccgttgc tgcaattcca cacccagttt aacgcggcgc tgccgtggga cagtatcgat
    16021 atggacttta tgaacctgaa ccagactgct catggcggtc gcgagttcgg cttcattggc
    16081 gcgcgtatgc gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat
    16141 gagcgtatcg gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa
    16201 gtctgccgat ttggcgataa catgcgtgaa gtggcggtca ccgatggtga taaagttgcc
    16261 gcacagatca agttcggttt ctccgtcaat acctgggcgg ttggcgatct ggtgcaggtg
    16321 gtgaactcca tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac
    16381 accatgacgc ctgcaacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg
    16441 cgtattgagc tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc
    16501 acctttgaag atttgcacgg tctgaaacag cttccaggtc tggccgtaca gcgtctgatg
    16561 cagcagggtt acggctttgc gggcgaaggc gactggaaaa ctgccgccct gcttcgcatc
    16621 atgaaggtga tgtcaaccgg tctgcagggc ggtacctcct ttatggagga ctacacctat
    16681 cacttcgaga aaggcaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg
    16741 attgcagtag aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac
    16801 gatcctgccc gcctgatctt caatacccaa accggtccag cgattgttgc cagcctgatt
    16861 gatctcggcg atcgttaccg tctgctggtt aactgtatcg acacggtgaa aaccccgcac
    16921 tccctgccga aactgccggt ggcgaatgcg ctgtggaaag cgcaaccgga tctgccaact
    16981 gcttccgaag cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg
    17041 aacctcaacg atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat
    17101 aacgacaccc gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga
    17161 tttcgtcgct aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta
    17221 tctggcctac gcgctgtgtt tttgtaggcc ggataagcaa agcgcatccg gcattcaacg
    17281 cctgatgcga cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata
    17341 agcaaagtgc atccggcacg aaggagtcaa catgttagaa gatctcaaac gcctggtatt
    17401 agaagccaac ctggcgctgc caaaacacaa cctggtcacg ctcacatggg gcaacgtcag
    17461 cgccgttgat cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt
    17521 catgaccgct gacgatatgg tcgtggttag catcgcaacc ggtgaagtgg ttgaaggtac
    17581 gaaaaagccc tcctctgaca cgccaactca ccggctgctc tatcaggcat tcccgtccat
    17641 tggcggcatt gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc
    17701 gattccagca accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg
    17761 caaaatgacc gacgcagaaa tcaacggtga atatgagtgg gaaaccggta acgtcatcgt
    17821 agaaaccttc gaaaaacagg gtatcgatgc agcgcaaatg cccggcgtcc tggtccattc
    17881 tcacggccca tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct
    17941 ggaagaggtc gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat
    18001 gcagcaaacg ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg
    18061 gcagtaatga ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata atcaagcctg
    18121 gttttttatt ggattttcaa cgtggcgcag gcaggtttta tcttaacccg acactggcgg
    18181 gataccccgc aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag
    18241 gttacgcttg caccgcaaga gtccgtggcg tttattcctg ccgatcaggt tccccgcgct
    18301 cagcatattt tgcagggtga acaaggcttt cgcctgacac cgctggcgtt aaaggatttt
    18361 caccgccagc cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa
    18421 aagcgcctgc gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc
    18481 tatctgatgg agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc
    18541 actatcgtta atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt
    18601 tctatagata ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc
    18661 gggcagcgca tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc
    18721 gaactggaat acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca
    18781 aaccacgatc ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg
    18841 caaaaacatg ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg
    18901 gagtggcgcg agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta
    18961 attatcgacg gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg
    19021 gaaactgtcg ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga
    19081 atggacgaaa ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg
    19141 aaagattgcg agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc
    19201 gaacgagcaa cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt
    19261 ggtcatctct attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa
    19321 gtgccaccgc acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat
    19381 tcagtgctgg tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt
    19441 gatcccgtcg ggctggttga aggcatggcg cagcctgatc cagagcacag taccgaaggt
    19501 tttctcgatg cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc
    19561 tggcacgggc gcgatgaagc caaacgtcag ggtaataaac cactttcgca ggcgctgaag
    19621 atcattatga atgcctttta tggcgtgctt ggcaccaccg cctgccgctt tttcgatccg
    19681 cgcctggcat cgtcgatcac catgcgtggt catcagatca tgcggcaaac caaagcattg
    19741 attgaagcgc agggctacga cgttatctac ggagataccg actcgacttt tgtctggcta
    19801 aaaggtgcac attcggaaga agaggcggcg aaaatcggtc gtgtactggt gcagcacgtt
    19861 aacgcctggt gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag
    19921 tatgaaactc atttctgccg ttttctgatg ccaaccattc gcggagccga taccggcagt
    19981 aaaaagcgct acgccgggct gattcaggag ggcgataaac agcggatggt gtttaaaggg
    20041 ctggaaaccg tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctatac
    20101 ctgcgcatct tccgcaacga gccatatcag gaatatgtac gcgaaaccat cgacaaactg
    20161 atggcgggtg aactggatgc gcgactggtt taccgtaaac gccttcgccg tccgctgagc
    20221 gagtatcagc gtaatgtgcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaac
    20281 caaaagcgcg gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc
    20341 accaacggcc cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg
    20401 acccgccagc tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct
    20461 acacttatga ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca
    20521 ttttgatgcg tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac
    20581 ctgaataaca ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga
    20641 tatagagccg aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa
    20701 gcgaattggg acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc
    20761 catctactgg tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt
    20821 tcaaccctgg tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa
    20881 aagaagaaaa tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg
    20941 tagccctgcg tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc
    21001 tgtttgccgg gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt
    21061 attccagcga acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga
    21121 ttccgcatca gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc
    21181 tggctgacga agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac
    21241 tgctctctgg cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt
    21301 ggctggtaga aatgctgcgc cgtttcaacc tgcgctttgc actgtttgat gatgagcgtt
    21361 atgccgaagc tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct
    21421 cgctggattt tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg
    21481 acctgctggt ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg
    21541 aatatcaggc cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga
    21601 ccccggaaca gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc
    21661 gtttccacga ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg
    21721 ccgttgccat gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg
    21781 agatgatcgg cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag
    21841 atgcccagag cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc
    21901 gcgtgctgtt ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca
    21961 ccattaaact gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg
    22021 gcgcacgtaa aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc
    22081 aggaatttga aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga
    22141 tgggctacct gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca
    22201 ctgcgctgca actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc
    22261 acgaaggtat gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca
    22321 ccggcgcaca ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg
    22381 ccagccacat ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg
    22441 gtcgtctgga tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga
    22501 aaaccgctca gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca
    22561 cctgcccgac cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg
    22621 cttcaccgga tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg
    22681 aagcgctgaa agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg
    22741 gtggcgaaaa agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc
    22801 tgatcgcctt cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg
    22861 acaacatgat cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt
    22921 cggaagatgg catcaccatc acctttgatc gtgaagtggc actggcgcgt gaagatgcac
    22981 agtttattac ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg
    23041 ataccggtag cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt
    23101 tggtggaact gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct
    23161 tcctgccacc gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc
    23221 aggtagagtt tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca
    23281 aactggttaa cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga
    23341 tcgagaaatc tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt
    23401 ctgccgagct gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg
    23461 aactgaccgc cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct
    23521 ggcgtctgga tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg
    23581 gatggaaaac tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca
    23641 cattatggtg gttaacaagc cgagcggttt gttgtctgtg ccgggtcgtc tggaagagca
    23701 caaagatagc gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg
    23761 tctggatatg gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga
    23821 gttaaaacgc cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg
    23881 gcatccatcc cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg
    23941 cccgaaacag aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt
    24001 ggagtatgcg gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca
    24061 tcaattgcgt gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc
    24121 atcaccagaa gcgagagcga tggcaccacg tttgttgcta catgcagaga tgttgacgat
    24181 tacccatccg gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc
    24241 acgctggcgc agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc
    24301 gattgcctga tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa
    24361 ccctttctgc tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc
    24421 catctccatc atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact
    24481 catcagctta cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag
    24541 cacattacag gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc
    24601 agattgctgc tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg
    24661 gtcaaactgg gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc
    24721 attcgggtgc agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg
    24781 aattaagtca aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg
    24841 gtaattatct gattttccca ctcggaacgc gttttgcgcc gcagtacggg aagcgccatg
    24901 aaggttcatt cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc
    24961 tttggatttg gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg
    25021 ctcacgctgg ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc
    25081 aattaacagg cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac
    25141 gccaatgatt tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc
    25201 tatctcccac aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat
    25261 cgcctataat ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct
    25321 attcggacag gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt
    25381 cacgcgcaac gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca
    25441 ctctcctggc caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc
    25501 tcgcctcaca gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata
    25561 ccaatgactt acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg
    25621 ccgtgtttac tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag
    25681 tgcagctcca tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc
    25741 tcggtaatgt ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga
    25801 atctgaacac caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg
    25861 gtcgcggtaa agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata
    25921 acggtagctt tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa
    25981 ttattcatga ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc
    26041 cggtaccgat cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg
    26101 gtttcttgat cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat
    26161 attactggaa catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc
    26221 gtggcaacat catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga
    26281 tggaactgga ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca
    26341 gttcacgtcg ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt
    26401 tcaacgtcga ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt
    26461 acggttccag tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa
    26521 acttcaatgc caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta
    26581 gctactcggc agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg
    26641 atacgcgtat ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag
    26701 caacccgtgt tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca
    26761 tcaataccga agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata
    26821 actccagaaa cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag
    26881 ttgacggcaa aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa
    26941 cgctggaacc gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata
    27001 actacgactc gtctctgctg caatctgact actctggcct gttccgggac cggacttacg
    27061 gcggtcttga ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat
    27121 atgatgatgc tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg
    27181 agtctcgcac tggcgatgac aacataacat gggagaatga cgacaaaacg ggttcactgg
    27241 tgtgggcagg cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc
    27301 agtacgatac acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg
    27361 atgaagaccg tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta
    27421 cgctgcctaa gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg
    27481 ctgtcgccag ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca
    27541 ccaatgcgaa caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg
    27601 caattcgcgt cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg
    27661 tatatgacaa cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc
    27721 tgggtacgca agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg
    27781 ttgatttacc acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg
    27841 aaaacgctgc ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta
    27901 gtcgataaag tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga
    27961 ttaatgcagt cggtaaaact gaacgctgct caggcaaggc agcaacttcc tgatgacgcg
    28021 acgctgcgcc accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg
    28081 cagaaaatgg gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg
    28141 aaacagaaca acatgacgct ggatcagatg cgcagccgtc tggcttacga tggactgaac
    28201 tacaacacct atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac
    28261 gaggtgcgtc gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg
    28321 ggtaaccaaa acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg
    28381 gaaaacccga cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat
    28441 caggcgcgta acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag
    28501 gcgctgaacg gcggccagat gggctggggc cgtattcagg agttgcccgg gatcttcgcc
    28561 caggcattaa gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc
    28621 ttccatattc ttaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa
    28681 gttcatgctc gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt
    28741 gtgaaactgg aacagattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca
    28801 gcgaaagagt tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct
    28861 acaccagata ttttcgatcc ggccttccgt gacgccctga ctcgcctgaa caaaggtcaa
    28921 atgagtgcac cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt
    28981 aatgtcgata aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt
    29041 aagttctcgg aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa
    29101 atcctgagca actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg
    29161 ggattggccc ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg
    29221 tttgtgccga tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc
    29281 tccgccctta ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac
    29341 ttcctgtcgc gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatggacatt
    29401 acgtggtgga gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc
    29461 tgatcacagg ccccgtgcat aaaggcgtca ttaacgacgc aggcattccg tttaccggtc
    29521 ataccgagtt tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg
    29581 aagaacttcg cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta
    29641 tcacccctgc acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat
    29701 ttggtattgc cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg
    29761 gtcatatggg tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc
    29821 agggaatgaa actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg
    29881 ataacgccga cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc
    29941 agggcttcgg gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg
    30001 accacggcac cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta
    30061 cggcgcttaa tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca
    30121 gggccactta gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga
    30181 cagcattgtt tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg
    30241 tctggcggca ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact
    30301 tgaccgcgat ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat
    30361 ttatcagcag gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc
    30421 gctgcgtgtt ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt
    30481 tagctatact gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg
    30541 tctggttgca ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta
    30601 ttgcaacgtg atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt
    30661 ggattccgcc gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt
    30721 tcgtgtgttg agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa
    30781 cagcctcggc aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat
    30841 gcgagcggaa aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa
    30901 cgcgcctttg caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa
    30961 agcgtctaca ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc
    31021 gtaaccatac gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc
    31081 accaatggca atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca
    31141 cttatcgcgc ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg
    31201 ggcaccatgc agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac
    31261 attcccgtat tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac
    31321 cttattggcg acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa
    31381 tttacccctg ggaaagatac cctctggctg acgggtgatc tggtcgcgcg cgggccgggt
    31441 tcgctggatg ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc
    31501 aatcacgatc tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat
    31561 cgcctgacac cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc
    31621 cagcctttgc tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg
    31681 ccgcagtggg atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg
    31741 agtgactcct atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca
    31801 ccggaattgc gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt
    31861 ttttgctttc caaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct
    31921 gccccactga aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc
    31981 tttggtcact gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat
    32041 accggctgct gctggggtgg gtcattaacc tgcctgcgct gggaagataa gcagtatttt
    32101 gtccagccgt cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct
    32161 gatataggaa ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct
    32221 atacaaaatt accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg
    32281 gcgtcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg
    32341 gtgtcgcctt ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg
    32401 aactgctcat aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc
    32461 gcgatggctt catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg
    32521 ctgaggataa tatttttgcg tcctggcaac ggacgaccga ttgattccca ggtatggcgg
    32581 cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg
    32641 ttccacggca tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga
    32701 ctgatcattg agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg
    32761 tcgtcgactg gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg
    32821 ctctctccct ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg
    32881 tgatttacgt cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc
    32941 cggtatgttt accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt
    33001 cgcgatcctc ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc
    33061 gggctttggt atcattctca accattgcca tctcttccac catctgaata ttaaagcggc
    33121 ggaacacatc ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca
    33181 gacgcccggt tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct
    33241 gacgcaaacg aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga
    33301 aatgttcttt caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa
    33361 tcagcacttc cgctttcgcc gctccggcag attccagtaa atccatccgc gtggcatcgc
    33421 cataaaacac tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga
    33481 gtaccaccat tttcaccccg ctggagagca gtaaacgtcc ggtaatctgc ccaaaacgac
    33541 cgaatccggc gataatcacg cgcggctgtt cttcgtcgat ctcatcggct tcacgcgctt
    33601 cctcagtaga agattgctca aggcgattga ggatcaccag cagaatcggc gttgctgcca
    33661 tcgacagcgc caccgccagg gtcagcgatt tcgcccactc cggctccagc acattcgcca
    33721 tctgcgccgc gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa
    33781 accaacgacg ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga
    33841 ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg
    33901 tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa
    33961 atggttcgat atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg
    34021 ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa
    34081 acacgaggaa taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac
    34141 gcagcgccgg acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca
    34201 cttttaacgc cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta
    34261 gcggaatcat cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg
    34321 cactgcgacc catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg
    34381 ccgtagagga gagcgccagc gtcatgccga tcaattccgc gacctgccag cgcagcccaa
    34441 gtaacatgca gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc
    34501 cgaacactgc cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa
    34561 acagcatcag caccacgcca atctcggcaa agtgcagaat agattcggca tcggtcacca
    34621 gtcgcagccc ccacgggcca ataatgaagc cggcgatcag gtagccaagt accgatccca
    34681 gaccaagacg taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct
    34741 gaatcagcgt atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag
    34801 tgacgcgcct gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac
    34861 ggtggcagcc agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca
    34921 tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc
    34981 cacagcaaat gtttgccatg cagcgccgtg ccgccgtgac cgtaagccca gccgtgcgag
    35041 aaaactttat cgatccaaag tttgaggagc ggtggaatgc tgtaccactg catcgggtgc
    35101 tgccagacga tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg
    35161 aagtcaggat agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt
    35221 tcaagcatcc gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga
    35281 atcataggta gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca
    35341 cgcggcttat agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca
    35401 ttctctgccc gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg
    35461 gggcaaacgc tacggaaatc aacaagccaa acagcgagat acccgccccc attagcatcg
    35521 tgttactgat accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc
    35581 caatacggct taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga
    35641 gttcattagg atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg
    35701 caaaggccat cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg
    35761 ccagcgtcat catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc
    35821 acagcatcgg cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg
    35881 ccgcgttttt gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa
    35941 tggcgaacat tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa
    36001 aatggcggcg attaaacaga tcgcgaaaac gggtttgctg cggttgctct tcatcgaaag
    36061 ccaccggttc gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc
    36121 gtaataacca gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg
    36181 ggatcgccgc gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc
    36241 cgaccagatc ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa
    36301 acgcccgctg acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg
    36361 caccgatgac aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa
    36421 acatcgtcgc caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc
    36481 gtccgacttt atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg
    36541 ttcccgcgcc caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct
    36601 ccagcgccac gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc
    36661 acagcaaaat gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt
    36721 gcatgggtta ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa
    36781 catcagccgt aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc
    36841 gccgacccca gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag
    36901 cgcacactgc cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc
    36961 tccagggcct gtttatcggc atcggccttc acaacaatgt gcggatgctc ttcatcgaca
    37021 ttgaatttat tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca
    37081 ctcctttcat gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga
    37141 ttttcttgcg catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc
    37201 ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt
    37261 tctggtacat acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca
    37321 gtttttgctt actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt
    37381 ccccggcggc aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc
    37441 cggcggcatc accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc
    37501 ctgcttccgg cactacgtga gcggaatatt ccaccagctt gccacccgcg atcagcggtg
    37561 caacggccgg atgttgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat
    37621 gcagatgatg cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga
    37681 agccgccgcc catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat
    37741 taccctgcaa ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca
    37801 cgccaaccgc cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga
    37861 tggagttcac tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt
    37921 ctacaccgac gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta
    37981 actgcgcgcc cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa
    38041 cggagtaaga acgctgggat ggcgaggttt cgtcaccatt gcagtagtcc atagtcatcg
    38101 ctgacttttc cgtcataaac gcgagttttt catgggtgat caggcgttct acgggggcgg
    38161 agtcggcgaa accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga
    38221 cgttcttggc acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg
    38281 cgagcaccag tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga
    38341 tatcttcgga cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt
    38401 ggatcaacgc gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat
    38461 gccgtaatcc gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt
    38521 ttgcgatgca ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta
    38581 cagttcaggt ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt
    38641 ttcgttttcc gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg
    38701 ttctgccagc gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg
    38761 ggctttgtcg agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc
    38821 cacagccgga gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg
    38881 agccgcatcg aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc
    38941 ttcgccaatc gccagaccac cgtaaaccat gtgtttcact gtcgctttac cgtcctgtac
    39001 gctgacggtg ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc
    39061 cagtaatttg ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg
    39121 aatagtgtca gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt
    39181 taatttccag acatgattag cgccgagctg gattgcctgt acgccgtcgg catcattgag
    39241 gacaaaggtg ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg
    39301 agaaggggta tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat
    39361 taaatgactt tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca
    39421 atcacgatgc gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct
    39481 gcctctgcgt taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg
    39541 ccgagaatgg ctttcatcga aggaatttgt ggggagttga tatcagtgga aacagcaaca
    39601 accgcaggca gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg
    39661 agggtatctg cagtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg
    39721 atttcgccca ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag
    39781 aggatcaaat caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc
    39841 ggcagtgcct gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat
    39901 agcacatctt tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc
    39961 acctgcgcct ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg
    40021 tattggctta ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc
    40081 gcaatatcct gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca
    40141 gaaatcatga aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt
    40201 aatttggaaa taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac
    40261 tttcaatatt gttaagttcc tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt
    40321 caatattgct ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca
    40381 caacataaca aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga
    40441 aacaaaaatg tgataacaat cacagaatgc agcttattga atacccatta tgagttagcc
    40501 attaacgcgt ccacgaggtt aataataatt atattaaatg ttaacaaaaa taaaacaaac
    40561 gggaacgcaa aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag
    40621 aaaaacggga atagaaccga aggttttctt tccgccgtta ataatcgtcg gcatactttg
    40681 ttggcttaca gtcagagatc tggatgcagc gaatgtcgtt attaatgctg tattcagtta
    40741 cgtcaccaat gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg
    40801 gttctggctg gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cacctgaatt
    40861 tagtaccgcc agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt
    40921 ctggggatcg attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa
    40981 ctcgacaggg gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc
    41041 gtgggccact tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat
    41101 ggaagtgatt cgccccagct cgacactggt gccgctggta ggtgaaaaac acgccaaagg
    41161 gttgttcggc actatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac
    41221 cagtctgggc cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc
    41281 gcataccctg caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg
    41341 cgtcgcttgc ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag
    41401 cttcctgatg ctgggttggg tgttcattgt cagcggtgcc agcttcatca tgaactactt
    41461 caccgattcg gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc
    41521 catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat
    41581 ttatgctatc cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga
    41641 actgtgcttc ggcatggtgc tggggctgac agcgtcaacc tggatcctgt ggactgtact
    41701 cggttgtaac actctgctgt tgatagataa aaacattatc aacattccaa atctgatcga
    41761 acagtacggt gtggcgcgcg ccattatcga aacctgggcc gctctgccac tcagcaccgc
    41821 caccatgtgg ggcttcttca tcctctgctt tattgccacc gttacgctgg ttaacgcctg
    41881 ctcttatacc ctggcgatgt ccacttgccg cgaagtacgc gatggtgaag aaccaccgct
    41941 gctggtgcgt atcggttggt caattctggt tggcattatc ggtattgttt tgctggcgct
    42001 cggcggcctg aaaccgattc aaactgccat tatcgccgga ggatgcccgc tgttcttcgt
    42061 caacattatg gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt
    42121 aacccccaaa atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact
    42181 gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga
    42241 gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat
    42301 cgacagtctg ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc
    42361 cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc tatgtactgt accagttgcc
    42421 gggcgggttc aacaccttcc tgcgcgaagg cacacaagag cagatcgaca agattatggc
    42481 tttccgcggc accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc
    42541 cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg
    42601 tagtaagtgt tttattacca gcagcgccta caccccgtac atcgtggtga tggcgcgcga
    42661 cggggcttct ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg
    42721 catcaaagtg accaaacttg aaaagctcgg tctgcgtatg gatagctgct gtgaaatcac
    42781 ctttgacgac gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa
    42841 ccgcgtcaaa gaagagttcg accatgaacg tttcctggta gccctgacca actacggtac
    42901 ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaac cagcgcgtgc agtttggcga
    42961 ggctattggt cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa
    43021 ctccatgaaa aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc
    43081 tggcgatgca gcgatgtgca aatacttctg cgccaatgcg gcatttgaag tggtggatag
    43141 cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg
    43201 gcgtgacctg cgtgtagacc gcgtctccgg gggatctgac gaaatgcaga tcctgacgct
    43261 gggtcgtgcg gtgctgaagc aataccgcta agtcaatgcg ctgcctgatg cgacgctgat
    43321 gcgtcttatc aggcctacgg gagcacgaat gtaggccgga taaagcgttt acgccgcatc
    43381 cggcagtcat gcgctacata aatttttcag gagcaaataa tggatcatct acccatgccg
    43441 aaattcgggc cgttggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg
    43501 tttgccgggc aaatgttcgc agaatggggc gcggaagtta tctggatcga gaacgtcgcc
    43561 tgggccgaca ccattcgcgt tcaaccgaac tacccgcaac tctcccgccg caatttgcac
    43621 gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa
    43681 accaccgata tcttcatcga agccagtaaa ggtccggcct ttgcccgtcg tggcattacc
    43741 gatgaagtac tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt
    43801 cagtacggca ccgaggagta caccaatctt ccggcctata acactatcgc ccaggccttt
    43861 agtggttacc tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc
    43921 gccgattact tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg
    43981 cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg
    44041 ggccagtact tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa
    44101 ggtaaagatc cctactacgc cggttgcggt ctgtataaat gcgccgacgg ctacatcgtg
    44161 atggaactgg tgggcattac ccaaattgaa gagtgcttta aagatattgg cctcgcacat
    44221 ctactcggta cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct
    44281 tacggcccac tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa
    44341 gttaaagaac gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa
    44401 ctggaaagca atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat
    44461 ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc
    44521 tggcgcggaa tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac
    44581 agcgaaaacg acattcagga gttggtcagc aaaggtctgg ccaaagttga ggactaaacg
    44641 cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat aacaaacgaa caaaaatgga
    44701 tagaggtgca atggatatca ttggcggaca acatctacgt caaatgtggg acgatcttgc
    44761 ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg
    44821 gtatagttat cttgagttaa atcaggagat taaccgcacg gcaaacctgt tttatacgct
    44881 ggggattcgc aaaggcgaca aggttgcact acatctcgac aactgcccgg aatttatctt
    44941 ttgctggttc gggctggcaa aaattggcgc gattatggtg ccgattaacg cccgcctgtt
    45001 acgcgaggaa agcgcgtgga tcctgcaaaa tagccaggcg tgcctgctgg tgaccagtgc
    45061 gcaattctat cctatgtatc aacagattca gcaggaagat gccactcaat tgcggcacat
    45121 ttgcctgaca gatgtggcac ttcccgctga tgatggcgtg agttcgttta ctcaactgaa
    45181 aaatcaacaa cctgccacct tgtgctatcg actgacgata cggcggaaat tctcttcacc
    45241 tccggcacca cctcccgacc gaaaggtgtg gtgattaccc attacaacct gcgcttcgct
    45301 ggatattact ccgcctggca gtgtgcactg cgtgacgatg acgtctacct gacggtaatg
    45361 cctgcgtttc atatcgattg ccagtgtact gcggcgatgg cggcgttttc tgccggggcc
    45421 acctttgtgc tggtcgagaa atacagcgcc cgcgccttct ggggacaggt gcagaagtac
    45481 cgcgccacca ttaccgaatg tattccgatg atgatccgta cgttgatggt gcagccgcct
    45541 tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag
    45601 caggaaaaag atgcgttttg tgaacgcttc ggcgttcgct tgctgacgtc ttatgggatg
    45661 acggaaacca ttgtgggcat tatcggcgat cgccctggcg ataaacgacg ctggccgtcg
    45721 attggtcggg cggggttttg ctacgaagcg gagatccgcg acgatcacaa tcgcccgctc
    45781 ccggcaggtg agatcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa
    45841 gagtattttc tcaacccgaa agccactgcg aaagtgctgg aagccgacgg ctggctacat
    45901 accggcgata ccggatactg cgacgaagag ggcttttttt atttcgtcga tcgccgctgc
    45961 aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc
    46021 acccatccaa aaattcagga tatcgtggtt gtgggtatta aagattcgat tcgcgatgaa
    46081 gccatcaaag catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttc
    46141 cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa
    46201 gatctgccac gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacag
    46261 cagggaacta ccctgccagc taataatcac gctataaaac aatatgcatt ttttgcaggg
    46321 atgttgtcgt ccctgaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg
    46381 acccgcaatg gatcaattct ggaaattacc cttgatcgtc caaaagcgaa tgctattgat
    46441 gcaaaaacca gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta
    46501 cgtgtcgcca ttattaccgg tgccggagag aagttctttt ccgcgggctg ggatttaaaa
    46561 gcggcagcag aaggcgaagc accggatgct gactttggcc cgggtggatt tgcgggatta
    46621 accgaaattt tcaatctcga caaaccggtt atcgcagctg tgaacggcta tgcctttggc
    46681 ggcggctttg aactggcgct ggcggcagat tttattgttt gtgccgataa tgccagcttc
    46741 gccctgccgg aagccaaact gggcatcgtt cctgacagcg gcggcgtgct gcgtctgccg
    46801 aagatcctgc cgcctgccat cgtcaatgaa atggtgatga ccggcagacg aatgggcaca
    46861 gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat
    46921 aacgcccgcg aactggcgca gcagctggtt aacagcgccc cgctggcgat tgcggcgctg
    46981 aaagagatct accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc
    47041 agcggcgtgt tgaaacacta tccgtcggtt ctgcattcgg aagatgccgt tgaagggccg
    47101 ctggcgtttg ccgagaagcg cgatccggta tggaaaggac gttaacaacc gtgagctatt
    47161 acgcctttga ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg
    47221 tcttgattgg cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc
    47281 gtggtgacta cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta
    47341 tgcatggcta ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcacggag
    47401 cgatcctgca tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtgatta
    47461 tggatggcgc ggtcattggc gaagagagca ttgttgccgc catgagcttt gtcaaagcag
    47521 gctttcacgg cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg
    47581 ttagtgacga cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg
    47641 ggcgctgtca tgcatcgtta catgaaacac agccactgag gcaaatggag gaaaatcgcc
    47701 cccgtttgca ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcgacctg
    47761 gttgctggat aagatgctac aacatcgcat ctggcaagca ggacgcccgg tatcaacgag
    47821 ataaaattaa cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag
    47881 ttccgtgagc ggaacggcag gaatacgcgg cgtctttctt acgccgacta atttatcccg
    47941 gctgttattg cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt ttaccagtcg
    48001 ctggcactgg catccccgtc cttccagctt attagggatc atcttaactt cgcagcttat
    48061 ttctgcgact tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc
    48121 gaaatgaata gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc
    48181 gattaacaag tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca
    48241 aattaaaagt ctggctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt
    48301 ggatatatcg atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt
    48361 catcaaaaat atcgattccc caaaagattt acttaatggg caaaaaaaac ggtcctcatc
    48421 agagaaccgt attacttatt cgatcaaatt agcggatgaa aaatatccgc catgacacgc
    48481 tattatttga tctgcgcgtg catttcctgc accgaaatta ctttttcggt cgcgtcggca
    48541 ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat
    48601 tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cgcgacggcc tgaagtggtg
    48661 ttgatgatgt aggtatattc gccattcttg atgcggtcct gaatgtgcgg acggccttca
    48721 tgcaccttgt tcaccagacg cggattaatg cccgcttcgc ccagcacaat cgccgtgccg
    48781 tgggtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt
    48841 tctttatcgc cttcgcgcac ggaaagcaac gcacgaccgt gtttcttcat ggtggagttg
    48901 ctgcccagct gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc catcacttcc
    48961 ccggtggagc gcatttctgg ccctaacagc gggtcaacac ccgggaattt attgaacggc
    49021 agcaccactt ctttcaccga gtagtacggc gggataactt ctttggttac gccctgctca
    49081 gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct
    49141 ttggagacga acggaacggt acgcgccgca cgcgggttaa cttcaatcag gtagacttcg
    49201 ttgtttttca ccgcaaactg cacgttcatc agaccgcgca cctgcaattc gaaggccagt
    49261 ttctgcacct gctggcgcat cacatcctga atttcctgac ttaaggtgta ggctggcaga
    49321 gaacatgcgg agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca
    49381 atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctaccgc gtcatcaagg
    49441 aagtgatcca gcaacactgg cgcatcgtta gacacgctga ccgccgtctg gaagtagcga
    49501 cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga
    49561 cgtaccacca gcgggtagcc aatctctttc gccttctcta ccgccatttc aatagcggta
    49621 acggtggcgt tcgccggttg tttcagtttc agacgctcaa ccgcatgctg gaagcgttca
    49681 cggtcttctg cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcagct
    49741 tccagcgcgc gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc
    49801 ggcttctcaa tacgcacgat ttctagcaca tcttccagag ttaccggctc gaagtagagg
    49861 cgatcggaag tgtcgtagtc ggtggagacg gtttccgggt tacagttaac cataatggtt
    49921 tcgtaaccgt cttcgcgcag cgccagcgag gcgtgtacac agcagtagtc gaattcgata
    49981 ccctgaccga tacggttcgg gccgccgccg agaaccatga ttttttcacg gtcggtagac
    50041 ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac
    50101 tctgccgcac aggtatccac gcgcttataa accgggtgca ggtcatactg gtcgcgcagc
    50161 ttgcggattt ccgcttcgcg cacgcccgcc agttttgcca gacgcgcatc ggcaaagcct
    50221 ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgctact
    50281 ttctcttcca gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatattggtc
    50341 aggttgaaca cgccgtccac agacaggccc gcacggaagg catcggcgat gtaccagata
    50401 cgctctgcgc cagcatcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatcg
    50461 aggctcactt tcgggtcgaa tccagtcgca ccgacttcca ggccgcgcag cgctttttgc
    50521 agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg
    50581 gtcagacggt cgttagcacc agcgaatttt tcgaagttga agcgaggaat tttagtaacc
    50641 acatagtcga tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc
    50701 agttcgtcga gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg
    50761 gtcgctttcg acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc
    50821 aggcgaccgt ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg
    50881 atttcacgca gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc
    50941 gtttgggctg gcgcgacagt gatggagtca ccggtatgga tgcccatcgc atcaaagttt
    51001 tcgatagagc agacgatgat gcagttgtcg tttttatcac gcaccacttc catctcgtac
    51061 tctttccagc cgatcagcga ctcatcaatc agcagctctt tggttgggga gagatccaga
    51121 ccgcgggcgc aaatttcttc aaactcttcg cggttataag cgataccgcc gccgctaccg
    51181 cccatggtaa aggatgggcg aataatgcac gggaagccca cgtcagcggc aaccgccagc
    51241 gcttcttcca tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc
    51301 gctacgtcga aacgacggcg gtcttctgct ttatcaatcg catcggcggt cgcaccaatc
    51361 atagtcacgc cgaactcttc caacacgccc tgacgctcca gctccagcgc gcagttcagc
    51421 gccgtctgac cgcccatcgt tggcagcacc gcgtccgggc gctctttttc aataatcttg
    51481 cgtactactt cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc
    51541 atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct
    51601 ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc
    51661 gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg
    51721 attacttagc ggttttacgg tactgctcaa ttaactcgat aaagtggtcg aacaacggcg
    51781 ctgcgtcgtg tggcccaggg ctggcttccg ggtgaccctg gaagctgaac gccggtttat
    51841 cagtgcgatg aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt
    51901 ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca
    51961 cgtttttctc cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga
    52021 cagtcttcgc accgctcgcc agcgccagca gctgatgacc gaggcagatg ccgaataccg
    52081 gaatatcggt ttcgaggaat ttctggatgg cggtaatggc gtaatcgcac ggggccgggt
    52141 cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag
    52201 aagtttgcgc cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt
    52261 tgcgcttggc accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg
    52321 cttctggcag gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg
    52381 tggtcacttc ttttgccaga tccatgccat tcagacctgg gaacgcgcgg gctttttcta
    52441 acgccaacgc cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct
    52501 cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc
    52561 gcttcaggta agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcagat
    52621 cgcgaatcac cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc
    52681 cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag
    52741 tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac
    52801 ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg
    52861 acttaatcaa aacaccctcc agagaatatt cactcacttt atttgcatat taattcacaa
    52921 tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc
    52981 tggagatatg ggcgctaaaa gtcaacttaa tggcgggatt ttttatatta ttttgtggct
    53041 catgcatgtt ttacaggcat taagcgacaa aaagatcata taacctgttc tggaaaacgc
    53101 ttgcgcagca agaagattgc atgaaataaa agcgagcagg gaaaagtgga ccaaatggtc
    53161 aaaatttaca ttaaaacaac aaaaaccgaa gacaaaacat gtaatttttg gcacttatag
    53221 acaatttaaa aacataacat taaaaataa
//
LOCUS       DAZFWP020000011        51642 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFWP020000011 DAZFWP020000000
VERSION     DAZFWP020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.011"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            171..1355
                     /gene="setC"
                     /locus_tag="ACHK0Z_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             171..1355
                     /gene="setC"
                     /locus_tag="ACHK0Z_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGU4500682.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANSQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1466..2389
                     /gene="yicL"
                     /locus_tag="ACHK0Z_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             1466..2389
                     /gene="yicL"
                     /locus_tag="ACHK0Z_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU4500683.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2393..3211)
                     /gene="nlpA"
                     /locus_tag="ACHK0Z_RS03075"
                     /old_locus_tag="pgaptmp_000615"
     CDS             complement(2393..3211)
                     /gene="nlpA"
                     /locus_tag="ACHK0Z_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU4500684.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3433..3726
                     /gene="yicS"
                     /locus_tag="ACHK0Z_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             3433..3726
                     /gene="yicS"
                     /locus_tag="ACHK0Z_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU4500685.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3767..4957)
                     /gene="nepI"
                     /locus_tag="ACHK0Z_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             complement(3767..4957)
                     /gene="nepI"
                     /locus_tag="ACHK0Z_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU4500686.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(5168..5620)
                     /gene="yicN"
                     /locus_tag="ACHK0Z_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             complement(5168..5620)
                     /gene="yicN"
                     /locus_tag="ACHK0Z_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU4500687.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5673..7007)
                     /gene="adeQ"
                     /locus_tag="ACHK0Z_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /gene_synonym="yicO"
     CDS             complement(5673..7007)
                     /gene="adeQ"
                     /locus_tag="ACHK0Z_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGU4500688.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            7182..8948
                     /gene="adeD"
                     /locus_tag="ACHK0Z_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             7182..8948
                     /gene="adeD"
                     /locus_tag="ACHK0Z_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU4500689.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8993..10384)
                     /gene="uhpT"
                     /locus_tag="ACHK0Z_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             complement(8993..10384)
                     /gene="uhpT"
                     /locus_tag="ACHK0Z_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU4500690.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10522..11841)
                     /gene="uhpC"
                     /locus_tag="ACHK0Z_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             complement(10522..11841)
                     /gene="uhpC"
                     /locus_tag="ACHK0Z_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4500691.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(11851..13353)
                     /gene="uhpB"
                     /locus_tag="ACHK0Z_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     CDS             complement(11851..13353)
                     /gene="uhpB"
                     /locus_tag="ACHK0Z_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU4500692.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(13353..13943)
                     /gene="uhpA"
                     /locus_tag="ACHK0Z_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             complement(13353..13943)
                     /gene="uhpA"
                     /locus_tag="ACHK0Z_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU4500693.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(14018..14308)
                     /gene="ilvN"
                     /locus_tag="ACHK0Z_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             complement(14018..14308)
                     /gene="ilvN"
                     /locus_tag="ACHK0Z_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4500694.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(14312..16000)
                     /gene="ilvB"
                     /locus_tag="ACHK0Z_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     CDS             complement(14312..16000)
                     /gene="ilvB"
                     /locus_tag="ACHK0Z_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU4500695.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(16106..16204)
                     /gene="ivbL"
                     /locus_tag="ACHK0Z_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             complement(16106..16204)
                     /gene="ivbL"
                     /locus_tag="ACHK0Z_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU4500696.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            16769..16858
                     /gene="tisB"
                     /locus_tag="ACHK0Z_RS03140"
                     /old_locus_tag="pgaptmp_000628"
     CDS             16769..16858
                     /gene="tisB"
                     /locus_tag="ACHK0Z_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU4500697.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(17121..17195)
                     /gene="ysdE"
                     /locus_tag="ACHK0Z_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             complement(17121..17195)
                     /gene="ysdE"
                     /locus_tag="ACHK0Z_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU4500698.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            17277..18461
                     /gene="emrD"
                     /locus_tag="ACHK0Z_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             17277..18461
                     /gene="emrD"
                     /locus_tag="ACHK0Z_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU4500699.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(18469..18966)
                     /gene="yidF"
                     /locus_tag="ACHK0Z_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             complement(18469..18966)
                     /gene="yidF"
                     /locus_tag="ACHK0Z_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4500700.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(18963..19325)
                     /gene="yidG"
                     /locus_tag="ACHK0Z_RS03160"
                     /old_locus_tag="pgaptmp_000632"
     CDS             complement(18963..19325)
                     /gene="yidG"
                     /locus_tag="ACHK0Z_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU4500701.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(19315..19662)
                     /gene="yidH"
                     /locus_tag="ACHK0Z_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             complement(19315..19662)
                     /gene="yidH"
                     /locus_tag="ACHK0Z_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU4500702.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            19771..20220
                     /gene="yidI"
                     /locus_tag="ACHK0Z_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             19771..20220
                     /gene="yidI"
                     /locus_tag="ACHK0Z_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU4500703.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(20267..21760)
                     /gene="yidJ"
                     /locus_tag="ACHK0Z_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             complement(20267..21760)
                     /gene="yidJ"
                     /locus_tag="ACHK0Z_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4500704.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(21757..23472)
                     /gene="yidK"
                     /locus_tag="ACHK0Z_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             complement(21757..23472)
                     /gene="yidK"
                     /locus_tag="ACHK0Z_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU4500705.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKIDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRSTPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLSMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            23639..24532
                     /gene="yidL"
                     /locus_tag="ACHK0Z_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             23639..24532
                     /gene="yidL"
                     /locus_tag="ACHK0Z_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4500706.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(24529..25343)
                     /locus_tag="ACHK0Z_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /pseudo
     CDS             complement(24529..25343)
                     /locus_tag="ACHK0Z_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25343..26959)
                     /locus_tag="ACHK0Z_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             complement(25343..26959)
                     /locus_tag="ACHK0Z_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4500707.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            27255..27971
                     /gene="yidP"
                     /locus_tag="ACHK0Z_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             27255..27971
                     /gene="yidP"
                     /locus_tag="ACHK0Z_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4500708.1"
                     /translation="MIYKSIAERLRIRLNYADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IGQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(27968..29629)
                     /gene="yidE"
                     /locus_tag="ACHK0Z_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             complement(27968..29629)
                     /gene="yidE"
                     /locus_tag="ACHK0Z_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU4500709.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(29826..30254)
                     /gene="ibpB"
                     /locus_tag="ACHK0Z_RS03210"
                     /old_locus_tag="pgaptmp_000642"
     CDS             complement(29826..30254)
                     /gene="ibpB"
                     /locus_tag="ACHK0Z_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU4500710.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(30366..30779)
                     /gene="ibpA"
                     /locus_tag="ACHK0Z_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             complement(30366..30779)
                     /gene="ibpA"
                     /locus_tag="ACHK0Z_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU4500711.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(30773..30845)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            31085..31417
                     /gene="yidQ"
                     /locus_tag="ACHK0Z_RS03220"
                     /old_locus_tag="pgaptmp_000644"
     CDS             31085..31417
                     /gene="yidQ"
                     /locus_tag="ACHK0Z_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4500712.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(31419..32633)
                     /gene="yidR"
                     /locus_tag="ACHK0Z_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             complement(31419..32633)
                     /gene="yidR"
                     /locus_tag="ACHK0Z_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU4500713.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            32734..33798
                     /gene="cbrA"
                     /locus_tag="ACHK0Z_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             32734..33798
                     /gene="cbrA"
                     /locus_tag="ACHK0Z_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU4500714.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(33795..35120)
                     /gene="dgoT"
                     /locus_tag="ACHK0Z_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             complement(33795..35120)
                     /gene="dgoT"
                     /locus_tag="ACHK0Z_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4500715.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVTYFIAIFGW
                     SVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQ
                     FVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKAELDY
                     IRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLTWFPN
                     YLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPIICGLL
                     ISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVFNFAG
                     GLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(35195..36343)
                     /gene="dgoD"
                     /locus_tag="ACHK0Z_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             complement(35195..36343)
                     /gene="dgoD"
                     /locus_tag="ACHK0Z_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HGU4500716.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(36340..36957)
                     /gene="dgoA"
                     /locus_tag="ACHK0Z_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             complement(36340..36957)
                     /gene="dgoA"
                     /locus_tag="ACHK0Z_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGU4500717.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(36941..37819)
                     /gene="dgoK"
                     /locus_tag="ACHK0Z_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             complement(36941..37819)
                     /gene="dgoK"
                     /locus_tag="ACHK0Z_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGU4500718.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(37816..38505)
                     /gene="dgoR"
                     /locus_tag="ACHK0Z_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             complement(37816..38505)
                     /gene="dgoR"
                     /locus_tag="ACHK0Z_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGU4500719.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            38783..39439
                     /gene="yidX"
                     /locus_tag="ACHK0Z_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             38783..39439
                     /gene="yidX"
                     /locus_tag="ACHK0Z_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU4500720.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(39485..40297)
                     /gene="yidA"
                     /locus_tag="ACHK0Z_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             complement(39485..40297)
                     /gene="yidA"
                     /locus_tag="ACHK0Z_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU4500721.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(40412..40810)
                     /gene="yidB"
                     /locus_tag="ACHK0Z_RS03270"
                     /old_locus_tag="pgaptmp_000654"
     CDS             complement(40412..40810)
                     /gene="yidB"
                     /locus_tag="ACHK0Z_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU4500722.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(41050..43464)
                     /gene="gyrB"
                     /locus_tag="ACHK0Z_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             complement(41050..43464)
                     /gene="gyrB"
                     /locus_tag="ACHK0Z_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU4500723.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(43493..44566)
                     /gene="recF"
                     /locus_tag="ACHK0Z_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             complement(43493..44566)
                     /gene="recF"
                     /locus_tag="ACHK0Z_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU4500724.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAQYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(44566..45666)
                     /gene="dnaN"
                     /locus_tag="ACHK0Z_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             complement(44566..45666)
                     /gene="dnaN"
                     /locus_tag="ACHK0Z_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU4500725.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(45671..47074)
                     /gene="dnaA"
                     /locus_tag="ACHK0Z_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             complement(45671..47074)
                     /gene="dnaA"
                     /locus_tag="ACHK0Z_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU4500726.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            47371..47451
                     /locus_tag="ACHK0Z_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             47371..47451
                     /locus_tag="ACHK0Z_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4500727.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            47681..47821
                     /gene="rpmH"
                     /locus_tag="ACHK0Z_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             47681..47821
                     /gene="rpmH"
                     /locus_tag="ACHK0Z_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU4500728.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            47838..48197
                     /gene="rnpA"
                     /locus_tag="ACHK0Z_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             47838..48197
                     /gene="rnpA"
                     /locus_tag="ACHK0Z_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU4500729.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            48161..48418
                     /gene="yidD"
                     /locus_tag="ACHK0Z_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             48161..48418
                     /gene="yidD"
                     /locus_tag="ACHK0Z_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU4500730.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            48421..50067
                     /gene="yidC"
                     /locus_tag="ACHK0Z_RS03315"
                     /old_locus_tag="pgaptmp_000663"
     CDS             48421..50067
                     /gene="yidC"
                     /locus_tag="ACHK0Z_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU4500731.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            50173..51537
                     /gene="mnmE"
                     /locus_tag="ACHK0Z_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     CDS             50173..51537
                     /gene="mnmE"
                     /locus_tag="ACHK0Z_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU4500732.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        12680 a        13875 c        13043 g        12044 t
ORIGIN      
        1 ttaattctta acatgctttt gctaataatc tcaattgctg gccctataat attgcgctag
       61 cattgctttc tggttgtatc agcgatactc aaaaattctt aatacaatac tcattcgact
      121 ggtacttatt tgtaactcag ttatattttt tcgcccggtg attcagaaga atgcaaaaaa
      181 cggctaccac tccatcaaaa atacttgatc tcactgccgc ggcattttta cttgtcgcct
      241 ttctgacggg tattgcgggc gctcttcaga ctcctaccct aagtatattc ctcgcagatg
      301 aactgaaagc ccgtcctata atggtaggtt ttttcttcac cggtagcgct attatgggaa
      361 ttctggtcag tcaatttctg gcaaggcact ccgataaaca aggcgaccgt aaattactga
      421 ttctgctatg ttgcttattt ggagtgctgg cctgcacgct ttttgcgtgg aatcgcaact
      481 acttcattct gctctcaacg ggcgtacttc tgagtagttt tgcttctacc gcaaactcgc
      541 aaatgttcgc cctcgcccgt gaacacgccg acagaacagg ccgtgagacg gttatgttca
      601 gtacattttt acgtgctcag atctcgcttg cctgggttat cgggccaccg cttgcttatg
      661 aactggcaat gggatttagt tttaaagtga tgtatctcac cgctgccatc gcatttgttg
      721 tttgcggact gatagtctgg ttgtttttgc catcaataca aagaaatatt cctgtcgtta
      781 cccaacccgt agaaatttta ccctccaccc acaggaagcg ggatacgcgg ctactttttg
      841 tggtctgttc aatgatgtgg gcggcgaata atctctacat gataaatatg ccgctattta
      901 ttattgatga actgcatcta accgataaac tggctggaga aatgattggt atcgctgccg
      961 gtctggaaat tccgatgatg ttaatcgcag gctattacat gaaacgtatt ggcaagcggc
     1021 tattaatgct cattgctatc gtgagtggaa tgtgttttta cgccagcgta ctcatggcga
     1081 cgactccggc ggttgagctg gaattgcaaa ttcttaatgc catcttcctt ggtattctct
     1141 gtggtatcgg catgctttat tttcaggact tgatgcctga aaaaataggc tctgcgacaa
     1201 cgttatatgc aaatacttca cgcgtcggct ggattatcgc cggctctgtt gacggaatta
     1261 tggttgaaat ctggagctac catgcgttgt tctggctggc gatagggatg ttgggtattg
     1321 cgatgatttg cctgctgttt attaaagata tttagctcac cagtctgggc taagaaaaat
     1381 cggcgtggcg caaacagagc catcctcgcc atttttgcta tcatgcctgc atacataaac
     1441 gacaaaacag tatgcagagg gaaaaatggg ttccaccaga aaggggatgc tgaacgttct
     1501 gattgccgcc gtgttgtggg gaagttcagg ggtctgcgcg caatacatca tggagcaaag
     1561 ccagatgtcg tcgcagtttt tgactatgac gcgtttgata ttcgccggtt tgattctact
     1621 gacgctgtca tttgttcatg gcgataaaat cttttctatt attaacaatc ataaagatgc
     1681 cattagcctg ctgatttttt ccgtggttgg cgcgctaact gtacagctca cttttttgct
     1741 aaccatcgaa aaatcgaacg cagccacggc aacggtgctg caattcctct caccgacgat
     1801 tatcgtcgcc tggttctcac tggtgcgtaa atcgcgcccg ggcattctgg ttttctgcgc
     1861 tattttgaca tcgctggtcg ggactttttt attggtgaca cacggtaatc cgacgtcatt
     1921 atcgatctct cctgccgcgt tgttctgggg cattgcctcg gcatttgctg ctgcattcta
     1981 taccacctat ccctcaacgc taattgcccg ctatggcacg ttaccagtcg tcggctggag
     2041 tatgctgatt ggcggtctga ttctgttgcc tttttatgcc agacaaggaa caaactttgt
     2101 cgttaacggc agtttgattc tggcgttttt ttatttggtg gtcattggta cgtccctgac
     2161 atttagtctg tacctgaaag gagcacaatt aattggcggt ccaaaagcca gcattttgag
     2221 ctgtgcagaa ccattaagta gcgcgctact ctctttgctg ttgctgggga tcacgttcac
     2281 attaccggac tggctgggaa cgctgctgat tctgtcatcg gtgattttga tttcaatgga
     2341 ttcccgtcgc cgcgccagaa aaataaatcg tccggcgcgg catgagtgag aattaccagc
     2401 caggcaccgc gccaccgtta aaaatggttt ctgccgcttt agcgacttcg ggtgattgat
     2461 aagattgcag aaattccttc acgttttctg cattcttatt atcttcccgt gccaccaaaa
     2521 tattcacata cggcgaattc ttatcttcaa taaatacgct gtcgtgcacc ggagaaagcc
     2581 ctgtctgctg aatgtaagtg gtactgataa tcgctacatc aactttggga tcatccagta
     2641 cgcgcggaag ctgcgccccc tcgagttcca taatctgcaa atgacgagga ttatcggtta
     2701 tatccagcgc ggtaggtaat aacccttttc cctctttcag agtaattagc ttctcttttt
     2761 gcaacagtaa aagtgcgcga ccaaggttgg tcgggtcgtt ggggatcgcc actgtcgcgc
     2821 cttcttttat ttgcgccacg gttttgattt ttttggaata acccgccata gggaagacaa
     2881 acgtatttcc caccgctacc agtttataac catgcgcctg attatcttgt tcaagaaagg
     2941 gtcgatgctg gaagacattc gcatcaagtt cgccatgatt tgtcgcatcg ttcggtagca
     3001 acgaaccgct aaatcctacc agttcaacat cgaggccata tttctctttt gccacctttt
     3061 tagcgacttc cgcgacatct tgttctgcgc catttataac gccaacttta atgtgttttg
     3121 catcgctgct actctggtcg caacctgcca gcagaattcc ggccagcaat aatgcggccc
     3181 ctgtccgtag atgatgtgtt gtcagtttca tttttttatc cttttattaa tgattacctg
     3241 actatagcgg taaggtcgct gcggtttaaa aaacgaaacg ctatcgataa gaataaaaag
     3301 gaataaaagt ggaatataaa gtctggcact tcttactgac cacgcaagcg cgtttcgtac
     3361 agcatgatga gagcgatgaa agcaaactac acttatgctt cattcgatac acatttgtaa
     3421 aaggttgaat ccatgaaacc aacgacacta ctgcttattt tcactttttt cgctatgcca
     3481 ggcatcgtat acgccgagtc gccttttagc tcactgcagt ccgcaaaaga gaaaaccacg
     3541 gtattacaag atttacgtaa aatttgcacg ccacaggcgt cattatcaga tgaagcgtgg
     3601 gaaaagttaa tgttgtcaga tgaaaacaat aaacaacata tacgcgaggc catcgtggcg
     3661 atggaacgta ataatcagag caattattgg gaagcgttgg gtaaggtaga atgcccggat
     3721 atgtaagaac agcaacaact actgaccaac gcttctttac ctgatatcag gatttcttca
     3781 ttttcacctt tgcagtaacc aacaatgcag tcagcaacat caatgtgccg gacaacatca
     3841 acggcgaagt cagaccaata ttatccagcg cataaccgcc gattgccgcg ccacaggtat
     3901 tagcaagctg aataaccgcc acctgaatag acccggcttt ttctgcctga tcggccagcg
     3961 agcgggtgat ccacgttgac cagccgacgg gaaccaatgc aaaagttagc ccccagataa
     4021 tcgccacgcc ggtagcaacg attttatcgc ttccccacaa cgtcagtacc aacgcactca
     4081 cagccagtat taacggcgcg cctgctaagg ccagttttac cgaacgttta agaatgaatg
     4141 acgaaagcga cgtaccgata aagctggcaa tgccaaaact caacagcacc agcgttaagc
     4201 catccacgct gaatcccgcc aggttcatat acaccgggcg aatatatgtg aagaaagcaa
     4261 actgcccggc gaaagacatg aagatggcga tcatccctgc catcacaccc ggacgttgta
     4321 ataagcggaa agtattttgt ttctgatgcg agggttcgcc tggcagtgaa ggcaatgatt
     4381 tgataatcca gaaaatacac agcacgccca tcaccgccgc cgcattaaag acattgcgcc
     4441 aaccgataag ctcgcctaaa aaactgccca acggcgcggc aatcaccagc gcaatagaaa
     4501 ccgcgccgaa gatcaccgac agcgccttcg gcaccgtacg cggcggcacc agacgcatgg
     4561 tcagcgacgc cgacatcgcc cagaacccgc ccagcgccag ccccagacag gcacgaccga
     4621 ttaaaagcaa actgaatgag ttagcaaagg aaaccagcaa gcaggagagc gtcagcaaaa
     4681 cggcaaacaa aataacaacg tagcggcggt cagtagcctg aattgtctgg gtaataaaca
     4741 aactggcaaa cattgccaca aaggcggtca cggtcaccga ttgcccggca accccttccg
     4801 aaatgcctaa atcctgggcc attggcgtca acaaactgac gggcaaaaac tcaacgataa
     4861 tcagacaggc gacacaaaac gccaccgaga aaacggctga ccagttcggt cgggtgatgg
     4921 catccgcgcc gcggttttcg gcaataaatt cactcatggt gttacccgtg gcatgtttca
     4981 ggggaaaagc cgaccacttt aacattgaaa tgtgtgacgc acttaacgtt tttgcaactt
     5041 ttacggtgtt gcttgagctg gagttaagat cgaaacggaa cagggcaaag attaccggat
     5101 gcggcgtgaa cgtcttatcc acccgacagg tcaggcgcgg tctgtaggct gataagacga
     5161 agcggtttta atgcagccag tgaacgccct cggcaggctg aaagaacgcg ttatagcgca
     5221 tctgaaaagc attaatatcc tgcatttcag gttcggtttc gcgcagaaaa cccagcgcca
     5281 gcctgacctg agcatcggta atcggcgcgg caagacgggc tttttgtaaa atccgatgcc
     5341 attgcagcag gttctgttcg ctaccatcga caatgacaat ttgccagatg agcaacacct
     5401 gcgcggcgtt ttgcaggtgc gactcatccg ggcgatgcaa ataacgtaaa aattcgtcac
     5461 cggctgactt tcccatttga tcgaccatcg actccacggg tacgacatcg atattcagcc
     5521 gatcgctgag acggcgaatc gcttttctgg ccccggatgt cagcacccga aaaccaacca
     5581 caaacactac cgccagcgtg gcgagcatta tccagatcat gagttctcct gcgtaatcag
     5641 aactcatgat atctggaaac cctcgccagt tactaatcca ccagaataat cttcagtgca
     5701 aacagagctg cgaccaccac cacacacagg ttcagatcgc gccagcgccc ggtgcacact
     5761 ttcatgatgc agtacgacat aaagccgagt gcaatccctt cggtgatcga aaaagtaaag
     5821 ggcatcatca ccgtggtaat aaacgcaggc accgattcgg taaaatcatc ccagttaacg
     5881 cgcgccaggc tcgaagtcat cagcacgcca acaaagatta acgctccggc ggttgcgtaa
     5941 ggaggaacca tcgccaccag cggtgagaag aacataacta acaggaacat aacgccaacc
     6001 acaaccgcag tcagccccgt gcgaccaccg actgccacac cagaagtact ttcaatatag
     6061 gcagtaacag acgaggtgcc gataaacgcc cccgccaccg aactgacgct atcaacatac
     6121 agcgccttat tcatattggg gaatttaccg ttactatcta ttaagcccgc tttatcagtt
     6181 acaccaatta atgttcctga tgaatcaaat aggttgatca gcataaagga gaaaatgata
     6241 ccggcgagtt caagtgttaa cgcgccgctc aaatctactt caccaatgac gccgctaata
     6301 tcaggcggaa tggaatagac gccgctaaaa tgaacatcac cgaaaaataa tccacagcaa
     6361 gacgtcacca caatagaaac cagcaccgcg gcatgaaaat gacgtgatga caacacggtt
     6421 ataataaaaa accctaaaat acctaacaac acgccgtgag aacttaaatc gccaatcatc
     6481 accagcgtgt ctttattggc gacaataacg ccagtatttt ttaatcccat taaggcgata
     6541 aataatccaa ttccgctggt gataccaata cgtaaactta agggaatgtt ggagatcatc
     6601 cagtaccgga tacgaaacag cgtgagcaaa aatagtccaa ctgcgcccca gaatattgcg
     6661 cccatcccgg tctgccagga gatgcccatc gcccccacga ccacgaaggc aaagaaggcg
     6721 ttcagcccca ttgccggagc cagcgccacg ggtaagttag caaatatccc catcgcaata
     6781 ctgccgatac cggcaatcaa acaggtggta acaaacacca ctttcgggtc catttgtgcc
     6841 gcgccgagga tttgcgggtt cacaaaaacg atgtacacca tggttaaaaa agtggtcatc
     6901 cccgcaatca attctgtgcg gacggtggtc ccatgctgag gtagtttaaa taatcgcgaa
     6961 agcgtccctg attcattact cacgtaatcg gtattgtcat tattcatttt tttgtccatt
     7021 ggaggagatt taatcccttg cttttgtaaa acaaatgaca acaacggttc agtgataatt
     7081 atcacatttc aattgcacat taatggatat tctttaataa tctcgcgacg tttctttatg
     7141 ataaataata atcaaattga taaaatcaaa atgagaaaaa tatgaataat tctattaacc
     7201 ataaatttca tcacattagc cgggctgaat accaggaatt gttagccgtt tcccgtggcg
     7261 acgctgttgc cgattatatt attgataatg tctctattct cgacctgatc aatggcggag
     7321 aaatttccgg cccaattgtg attaaaggac gttacattgc cggtgttggc gcagaatacg
     7381 ctgatgctcc ggctttgcag cggattgatg ctcgcggcgc aacggcggtg ccagggttta
     7441 ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcactttt gaaaccgcta
     7501 ccctgccgcg cggcctgacg accgttattt gcgaccctca tgaaatcgtc aacgtgatgg
     7561 gagaagccgg attcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa aaccagtact
     7621 tacaggtcag ctcttgcgta cccgccctgg aaggctgcga tgttaacggt gccagtttta
     7681 cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt gcagaaatga
     7741 tggactaccc tggcgtaatt agcgggcaga atgcgctgct cgataaactg gatgcatttc
     7801 gccacctgac gctggacggt cactgcccgg gtttgggtgg taaagaactt aacgcctata
     7861 ttactgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga cgccggaaat
     7921 tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgcccgcaat ctcaacgcgc
     7981 tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtacc gatgaccgta
     8041 acccgtggga gatcgcccat gaaggacaca tcgatgcctt aattcgccgc ctgatcgaac
     8101 aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg gcgcgccact
     8161 ttggtctgaa tcacctcggc ttactggcac ccggcaagca ggccgatatc gtcctgttga
     8221 gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgagccg attgatgcgc
     8281 aaaccttaca ggcggaagag tcggcgagac tggcacaatc cgctccgcca tatggcaaca
     8341 ccattgcccg ccagccagtt tccgccagcg actttgccct gcaatttacg cccggaaaac
     8401 gctatcgggt cattgacgtc atccataacg aattgattac gcactcccac tccagcgtct
     8461 acagcgaaaa tggttttgat cgcgatgatg tgagctttat tgccgtactt gagcgttacg
     8521 ggcaacggct ggctccggct tgtggtttgc ttggcggctt tggactgaat gaaggtgcgc
     8581 tggctgcgac ggtcagccat gacagccata atattgtggt gatcggtcgc agtgccgaag
     8641 agatggcgct ggcggtcaat caggtgattc aggatggcgg cgggctgtgc gtggtacgta
     8701 acggccaggt ccaaagtcat ctgccgttgc ccattgccgg cctgatgagc accgacacgg
     8761 cgcagttgct ggcggaacaa attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt
     8821 tacccgatga gccgtttatt cagatggcgt ttctttctct gccagtgatc cccgcgctaa
     8881 aactaaccag tcaggggcta tttgatggcg agaagtttgc cttcactacg ctggaagtca
     8941 cggaataata aaaaagcccg gcgtcatgcc gggcaaaagt caccagttac gtttatgcca
     9001 ctgtcaactg ctgaattttt ttctcgcggc ggattttgcg ttcttccatc accgccacta
     9061 tcgccatcag gcagatacaa ccaatcgcgg cgatatcaag tgcggcgaag gtgcctgccc
     9121 agccggtaag gccgaatacc ggcgtcccat cggcaatcat tcccagacct aacttggcaa
     9181 agctgtcacc aatcaggtaa gcaaaggtgc ctttaatacc atcggcagcg ccaatcgctt
     9241 ttttaggtac aaagccaaca gcagccacac caatcaacaa ttgcgggcca aagaccagga
     9301 aacccaacgc aaagagagaa gccagataga tatattcgtt gctggcatgt tgatacacac
     9361 cgagcgtggc gataatcagc gccagcgcga tgcaggccac caggccacgg cgaccgttcg
     9421 ccaggtcaga gagccagccc cacagcagcg taccgaccag cgcaccagct tcaaacagcg
     9481 taaagccctg aatcgccacc gctttagaga gtttcagttc ctggaacgcg tatacggttg
     9541 accactggtc gataccaata cgtaccacat agaggaaaat gttggcgaag cacagcagcc
     9601 agatcacttt gtttttcagc acatactcaa caaagatctg ccacttggtc atatcggtag
     9661 attctgtctc tttgtcctct tcgctgatct cctcgccgaa cagttcttca gctttgccga
     9721 ggccataaga ttccggggag tcgctgccgt aacgcaggcc gataaaaccg acaatcagcg
     9781 cgataatcga cgggaagata aacatgccga tgacatggcc atcgaacagg taatttgccc
     9841 cgaacagcgc cacacctgct gcgcctgcac cgccaaggtt gtgagaaata ttccagaaac
     9901 cgaggaatgt cccgcgttta cgacgcggcg tccatttggt gatggtggag taactgcacg
     9961 aaccgccggt actctggaaa aagccgctta aggcgtagaa ggcaatcatc aggaacaggc
    10021 taaccgagcc gctgcccata ctggcactga agcccagcat acaaatggca gacaggatca
    10081 gcatgaacgg caggaattgt ttggtgtttt tgccgtcggc gtagtaggaa accagcgttt
    10141 tacccacgcc ataagtgatg gagaaaccca ggccgatcat ccccagctgc gtcatgctca
    10201 acccgtaggt cgagatcatg tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca
    10261 tcgtcaggta gccgataaag accaccaggt aggattgcat gaacggtttg aaccacattt
    10321 tgcgccgcac ttcgagcgga aggtccaggg tcggcttgcg aacctggttt aagaaagcca
    10381 gcatgggtta ctcctgaaat gaatacctgc gtgaggcatg cattgtaaaa atcagcagca
    10441 aatcaggcct gagatagcgt ccaggcaaaa cctggaaaat ttcttagttt tgccggatat
    10501 gaagtgaaaa ggtgagatgc atcacgcttc gcgcggtgcc tgggcgttca aaaagggcag
    10561 taacagcagt gcggaaatcc cggcggcgat agagataacc acaaaaaatc cgctccagtg
    10621 ccaggtatcg agtactttcg ccagcggcca accagcaagc gacgccccca gataagcaaa
    10681 caagccgaca aaccccgtcg ccgcccctgc cgcctctttg tgggaacact ctgccgccgc
    10741 catgccgatt aacatctgcg ggccaaagac aaaaaaacca atggtgaaga agcaggttgc
    10801 ctgcatcacg tagctggcaa atggcatcag ccacagcgag ccgactgaaa gcaaaattcc
    10861 ggcggcgaaa atcaaattca tcggcccacg gttgccgtta aacaatttgt ccgagcccca
    10921 accggctacc agcgcaccga taaatccgcc cagttcaaac atcgtcactg ccgtattcgc
    10981 cgtgaccaga tcgacgccca gtgtctcgga catatacaaa ttgccccagt cgttgatcgc
    11041 cgcccggacc acatagacca gcacatagca aaacgaaagc agccagatat acggattcag
    11101 caacacatat ttggtgagga tctctttacg cgtcaaccct gccccttctt gttgttgagc
    11161 aatttccagc gcgtcgtgtc gccattcacc gaccgccggt aaacctaacg cctgcgggcg
    11221 atcgcgtagc cgccagcaga gaaaaatccc cacgactatc gccatacaac cagcaatcat
    11281 catcccggca cgccagccgt aatgcagcgc agccgctgcc atcacaatgg gaatgagtgc
    11341 gccgccgacg ttatgcgccg tgttccataa tgcccaccaa ccgccgcgct cggtacgtga
    11401 ataccaggcc gttaacagac gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt
    11461 cagcacccag agcacggcaa acgcccatag cgacgtcgag aagccaaaca gaatgttgat
    11521 gatgcccgtg gcgataagcc ctatccccat aaaataacgg gcatttgagc gatcgctgac
    11581 aatgccggag acaaacttcg acacgccgta ggtaatgtaa aacagggtcg ctaacaggcc
    11641 gatatcgcta cggctgagca cgccgttagc aaggatttct ggtacggcgg cgttaaaact
    11701 tttacgcgtg aagtaaaaca gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg
    11761 ccagtagcga tagcgggcat caatttcata tttatcagtc attaatggcg catcggcagg
    11821 cgctttcaga aacggcaaca tcatcaaacc ttagacatag cgttgaggta gagaaacgct
    11881 gacacgcgtg ccgtgcagac aggaaatgtg taatgtgcca cccagcgccg ttacgcgctc
    11941 gcgcattccg gtgaggccaa aaccttgttg cccggaaccc ggcggcaaac cgctgccatc
    12001 gtcttcaata accagcatca accgttcatc ctgctgccag ccttgcagcg tgaccgcgct
    12061 ggcatcagca tgtttcacaa tgttgttcag cccttcctgg cagacacgaa acagcgtcac
    12121 gcgctggttt tcacttaatg ctgattcatc gattcgccat tcgagatggc tgacaatacc
    12181 gcgcccttcc agctccattt cccgcatcag tgagcggatg gcctgctcca gggtgagatc
    12241 atccaactgg cgcggacgta accgacccaa caaacggcgc accgcgtcgt aaacgcccag
    12301 cgatagttgt tcgatgagct gcccgctctg cttcacgctg gcgttatctg ccgccagccg
    12361 ctgaacaatg cccgcctgag tacgaatagc agtgatggtc tgaccgatat catcatgcag
    12421 ctcacgcgcc acatcacgac gcacgctctc ttcggtttcc agcaaccgtt cagccagatg
    12481 ctgattgcgc gccagttcct tttgcagcga ctggttaagt tcacgcaacc gctggatgcc
    12541 agcgcccagt aacaaccctg tcagactttg caccagcaac gagagcaata aatccaccgg
    12601 atgatcgcgc caggtttgac tggcgatcag cgcgatggcg ttcatcaacg tcgcgatcag
    12661 cgccccttgc caaccgtagt gccaggccag cgcgataatc ggcagcgcca ggcagaatgg
    12721 cgtaaagcgc gacagttcgt ccggcaatcc caactggagc cagagactaa taacaaacaa
    12781 cagcaagtac cagaccagat gccgcccgcg ccagttgatc ggttgagaaa ccagtgacgg
    12841 gccgagcggc agccaggtgt tattggcgag atagtgccag aacaccagac atatcggggc
    12901 cagcgtcagg ccgccagtta aagtcagcaa cagcgcattc cacgactctt tgccgtgcca
    12961 aagccagggc agcgactgca acaacgccgc cgccgttaac gccgccccct gtaacagcaa
    13021 ggtgcgccag tcacgctgat ggcgatagcg cgagatcagc gctacgggca gtaacgtcag
    13081 taaactaccg atcatcaata acggaaaatg ggttaaaccg accgcctgcg ttagccagta
    13141 aatcagcagc cattccgcac ccagcaatac gggccagtat ccgcgcgggc attgcagcat
    13201 tagccctagc cgcagaccaa acggaaataa cagcaccgcc atatcagggc gctcaaccag
    13261 gtgcaggctg atactccaca ggcaaaacca tgcggcagag aagataaaaa agcaggcaat
    13321 aacggtaatt aagcgggaga acaacgtctt catcaccagc catcaaacat gcggcgcgcc
    13381 agttctacgt cgttactgac gcccagtttt tccatcagat tggcgcgatg gacgtgtacc
    13441 gttttcggtg acaagcccag ttcggcggca atctctttca ccgccattcc ttgcgccagt
    13501 ttttccgcca cctggcgttc acgtttggtc agcgggtcct gacgaccgga tgccagttta
    13561 atggcaatat ccggcgtcag ataacagccg cccgtagcaa ccgtatgcac cgccgcaatc
    13621 agttcgtcag ggctacagcg tttggagaga aagccgcgcg cccccgcgtt aagcgcctgc
    13681 tcaaccagcg ccggactgtc atgaacagag agcattattg tcgccatacc tttcggcagc
    13741 tggcttagca gttccagacc ggagatatcg ggcatggaga tatcgcaaat acacacctgc
    13801 acaccgcgcc ccggcagccc cgccagcgcc tcgcgccccg aaccaaactc ggcaactacc
    13861 tgcaaatcag gttccagccc cagcagctgc gcaaagccgg agcggacgat gaggtgatcg
    13921 tctataaggg caacggtgat catggtcttg tcctggcggg taaaaaaaaa cgcgcttacc
    13981 ttaacgataa gcgcgatgtt gttcaagcct tgagcggtta ctgaaaaaac accgcaatct
    14041 tgttaaacat agtcggatcg gactgattac gctgcacttt caccacatct tccagcttat
    14101 cgatttggct tatcatctgc tccagacgct ggtcgtcatt gaccagtagc cagatatggc
    14161 ttttgtcgct gtcctgaatc ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg
    14221 caaaaaggcc acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg agctccagaa
    14281 tcacgttgtc atgagttgtg ttttgcatgg cttattcccc caccatttca gtattcgccg
    14341 cacctggcgg caccatcggg taaacttttt cttcggcatc aatgcgcaca tggatcagcg
    14401 ccgggccagg gcgattgatg atttcctgca atgcagcctg cggatcggct tcgttattca
    14461 aatcacaggt ttcgaggccg aatccggcgg caatctgcat aaagttgatt tttcccggat
    14521 aggtggcggc aaaaacgcct tgctcgtaga acagactctg ttgctgatgc accagcccca
    14581 gcgcttcgtt gttcatcaga atgattttga catccagctg attttcactg gcggtcgcca
    14641 tctcctgaat attcatcatc aggctgccgt cgccggagaa acacaacact ttgcgatccg
    14701 ggttcgccag cgccgcgcca atcgccgcag gcaggccaaa acccatcgtg cccagcccac
    14761 cggaggtcag ccactggcgt gggcgattga gcggataagc ttgcgcggtc cacatctgat
    14821 gctgacccac atcggtggtg ataatcgcat tgtcatcgac acaggcggca acggcgttga
    14881 tcaggccgta atggcttaat ggatcgcacg ctttcgggat tggacacggg aattcgcgct
    14941 gcaaatccgc taccaactgg tgccactctg cacgcggttg cgcttccacc tgcgggatca
    15001 attgcgccag cacgtcatca acatccgcct gaatcgccac atgcggctgc ttgattttac
    15061 ccagctctgc gcgatcgata tcgacatgaa taattttggc attcggacag aactgctcgg
    15121 ttttgccaat cgcccggtca tcaaaacgcg caccgagcac tatcaacaaa tccgcctcct
    15181 gcaaaatata gttggtgctg cgcacgccgt gcatccccag catacccagc gacaacggat
    15241 gcgcttttgg caacatgccc agcgccatta aagtcatggt ggtaggcagt tgcgctttct
    15301 ccgccagttc acgcacccgt gcgggcgcat tgatcacacc gccgcccaga taaagcaccg
    15361 ggcgtttggc agcgttaatc atcgccgctg cgtcacgaat gctttcttcg ctaaaggcgg
    15421 gggcggcggc tttttctgcc atagcgggct gtgtttcaat ctcaaaaact gccgtttgca
    15481 catccttagg aatgtctatc cacaccgggc ctgggcggcc tgattgcgca atgcggaagg
    15541 catcgctcat gacctgcggg agttcttcga tatgtctgac cagatagttg tgtttggtga
    15601 tggggataga gatgccgtag gtgtccactt cctggaaggc gtcggtgccg atcatcgagg
    15661 cgggaacctg accggtgatg caaatcagcg ggatggagtc cagccgcgca tcggcaatgg
    15721 cggtcaccag gttagtcgca cccggtccgc tacaggccat acagaccgcc ggtttaccgt
    15781 cggtgcgcgc catcccctga gcgataaatc ccgcgccctg ttcatgacgg gccagaatat
    15841 ggcggatttg cgtgctttgg cttaaggcat cgtaaacagg caggatagaa ccgcccggaa
    15901 tgcccgtcac aatcttaatg ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg
    15961 taaagcgctt acgcgtcgat gttgtgcccg aacttgccat gctccagtcc ttttcttctg
    16021 ggccgacttt ccgggaggtg cttaaacgaa aaaccccgcc cggtttgcgc cggcggggtt
    16081 ttggaatcgt gtgttgttcc agtccctacg gcgcattgcc gacgaccacc accacacgca
    16141 cgacgaccac tgcggcggat agcgcagttg gtagtagttt tgcgttgaac atggaagtag
    16201 tcattgggga ccttgttggt tttgtgttta acaatattta tacaagcaca gctttacagg
    16261 ggagacaatg gaaaattttt cagcaaggga aaattgaggg gctgatcacg ttttgtactg
    16321 aattgcagat aacaaaaaac cccgccggag cgaggtttca tcagccgcct gcggctggta
    16381 accgcaaagc acactgtatt atgtcaacac tgaaagtata cgtgttccgc gcagaacgcg
    16441 caatttcggc acgaattttg acgtatttag ggcatagttg agtatcgatc acagtttgcg
    16501 ttttgtccaa atattactgt ttatttatac agtaaacttc tataatatca ctgtacgcaa
    16561 tgtgttatgc gggggccgca tcgttacccg gcgcactaag tcctggctga aacgggtggt
    16621 gccgtcagcg ccttaacccc gcgtgagcac actgtgttat gtcaacaagc acaacgtttc
    16681 tccttaagat accgcgtgca caacagctgg caacaggcag cggaaaggta cgtcagctgg
    16741 cagtgctcct gaaccacagg agacgcgtat gagcctggtg gatatcgcca ttcttatcct
    16801 caaactcatt gttgcagcac tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt
    16861 cagattcaag tcgcaccaaa ggggagcggg taaccgctcc ccttttatat ttagcgtgcg
    16921 ggttggagtt gggggcttct tctaccagca gaatgtttgc acctgcgctg cctgtgcccc
    16981 tcaccctagc cctctcccca aaggggcgag gggaccgtcc cgtgcaatgt ttagctcatt
    17041 ttctgaaagg gaacggtggt ttccccctcg cccctttggg gagagggtta cagttcgcac
    17101 gtaatccctc ccggcaacat ttattgccgc ttttgtttac atattctgcc gctaaacaat
    17161 tctccattcc tggcgtatat ctggctaaca ttcatcaatg tgatagattc ctctcccgca
    17221 tttatgggaa tgcgtagtga cttattctaa ttatttttat aaaagcatcc gtgataatga
    17281 aaaggcaaag aaacgtcaat ttgttattga tgttggtatt actcgtggcc gtcggtcaga
    17341 tggcgcaaac catttatatt ccagctattg ccgatatggc gcgcgatctc aacgtccgtg
    17401 aaggggcggt gcagagcgta atgggcgctt atctgctgac ttacggtgtc tcacagctgt
    17461 tttatggccc gatttccgac cgcgtgggcc gccgaccggt gatcctcgtc ggaatgtcca
    17521 tttttatgct ggcaacgctg gtcgcggtca cgacctccag tttgacggtg ttgattgccg
    17581 ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt aatggcgcgt actttgccgc
    17641 gagatttata tgaacggaca cagttgcgcc atgctaacag cctgttaaac atggggattc
    17701 tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct ggatacgatg tggaactggc
    17761 gcgcctgtta tctctttttg ttggttcttt gtgctggtgt gaccttcagt atggcccgct
    17821 ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg cctgcttacc agttataaaa
    17881 cgcttttcgg taacagcggt tttaactgtt atttgctgat gctgattggc ggtctggccg
    17941 ggattgccgc ctttgaagcc tgctccggcg tgctgatggg cgcggtgtta gggctgagca
    18001 gtatgacggt cagtattttg tttattctgc cgattccggc agcgtttttt ggcgcatggt
    18061 ttgccggacg tcccaataaa cgcttctcca cgttaatgtg gcagtcggtt atctgctgcc
    18121 tgctggctgg cttgctgatg tggatccccg actggtttgg cgtgatgaat gtctggacgc
    18181 tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat gctgtttccg ctggcgacca
    18241 gcggcgcgat ggagccgttc cccttcctgg cgggcacggc tggcgcgctg gtcggcggtc
    18301 tgcaaaacat tggttccggc gtgctggcgt cgctctctgc gatgttgccg caaaccggtc
    18361 agggcagcct ggggttgttg atgaccttaa tgggattgtt gatcgtgctg tgctggctgc
    18421 cgctggcgac gcggatgtcg catcaggggc agcccgttta agcgcacgtc accgcagcat
    18481 cgtcatcagc tccatgggag aacgatgctg ctttatcaga tcacgcatca cccgcatatg
    18541 cggtgcggag taagaataaa acgcctgata gcccgcacaa agcacgctgt ctggcgtctc
    18601 ctggcgagcg tgcgggcaca ggcgatgcca gtcgcacgcc tggcagtcac ggcgcggttg
    18661 cgggttttcg ctgccacacc atttttgtaa ggtggcgtca aaaagctcaa tcgagatacg
    18721 actgatatct gcccgcaccc aacactcgaa cagggcaatt aaaaatttcc cccacagctc
    18781 tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca aattcaagat agggaatgag
    18841 ctgtacggag cggatgcccc gctgtttgaa attgtgataa aaatcggcag gttgcagagc
    18901 atccttgtac tctacaacca gtttatgcct gtcctcttca gcatcaatga cctgacttcc
    18961 tgtcatgcga ctctctcaat aaatacgata agttgatgta tatgcgttac agcaaacagt
    19021 attgcgagag ataacaccgc gagggagatc aaaaatttgt cacggactac gtgaaattgg
    19081 gaaaaatcgc tattcgtgac atccattaaa ttgcgattac gggtgtagtg ccagaggatc
    19141 agcgccacga tggcgagaat gccaatcgaa atccagaata acatgcccgc ctggtgccag
    19201 ttgtgtttga tagccaacgc catcaacgcg ccgtagccca gcatggtacg aaaccacgcc
    19261 agtgatgtgc gctccggctg cagtcctggg tcggcaatgc gtctggcttt gcggctatcc
    19321 ggcatacaac accagtccca taacgatcac cgccacgacc atcaatatta agctaatgat
    19381 taataagctg ttggtgtagg gcaggtcctc tttcagacgc atcgcttttt cattgcgtaa
    19441 ccagcgcagg tagccataca tcgctaaccc accggaaaat agacacaaca acaacgccag
    19501 cagttcgcga atgacgggcg tggcgaaatc tggcgcaagt tggtcaagcc caacccccgc
    19561 cgccagaaat cctagtgcgg tacggatcca cgccagaaaa gtacgctcat tagccagcga
    19621 gaagcggtaa tcgggcgctt ctccgaggcg ggaaattttc attgttgctc ctttgcaatt
    19681 tcactaacgc tctatcttta gcggtttttt ttaagaattt cgtccgcccg tttcctgata
    19741 tgcttttgcc atcccctaca aggaggtcag atgggtatca tagcgcaaaa taaaatcagt
    19801 tcattgggca tgctgttcgg tgccatcgct ttaatgatgg ggattatcca cttttcattc
    19861 ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg tggcggacaa aacggctgaa
    19921 attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa ttactaccgt ggagaaaaaa
    19981 gaggacgtag atgtcgataa aatcctcgat caaagcggta tcgcactggc cattgctgcg
    20041 ctactttgcg catttattgg cggtatgcgt aaggaaaatc gctggggcat tcgcggcgcg
    20101 ctggtttttg gcggcgggac gctggctttc cataccttgc tttttggtat cggcatcgtg
    20161 tgcagcattt tgctgatatt ccttatcttt tccttcctga ctggcggttc actggtttaa
    20221 gcagcctgaa taatgctcgc cgggtgttca tcagcgggca ttatcgtcag aacttctgtt
    20281 ttttctcttc cactttcact ccttgcgtcg gtaggccggt gtcatagtca cgtaccaccg
    20341 gcgaatagcc atcttgtggg cgtggacgaa atgcccccat ccagcgcggc agtgcatctt
    20401 tacgccacgg acgcaggctc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    20461 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    20521 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    20581 tgagtaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    20641 tgtcatgctc aatctcgtag cggttaaatt ccaccattac accgcgtggc tctttcacgg
    20701 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    20761 tcggcagtaa atcgatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    20821 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    20881 gcttatgtgc gcccatcatt tcgccgtgat cggaagtata aatgacccac gtattttcac
    20941 gttgctctgg cgttaaggca ttgatgaccc gtccgatttg gtcatcaaca aagtcattac
    21001 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    21061 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgagct ttctcgccca
    21121 gatcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    21181 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcgcg ggctgttgca
    21241 gaaaatctac cgcccgattg ctgatgcggt gcgcccaggt gaaggtttcg tcgatatggt
    21301 tcgcctgtaa atcctcaacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    21361 tcagttcgct aaggtagttc gccccatcga accagtaatc agcgtcccac tccggcggac
    21421 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    21481 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    21541 tgttggtcca cgggccggac tggttagcgt agataccggt aaatagtccg gcgcgtgcag
    21601 gcgtacaaac cggtgaacag gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    21661 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    21721 gggtatcggt catgatgaac agaaaattgg ggcgtttcat aggctatcct tcgcgtcggg
    21781 agtaaaggtt acggctgggt cgtgacgatg ccgccagctg tcaaaaatca ggtagacaat
    21841 cactacggcg gcaatgaaat aactgatcat cgataaccag cgcgtaccgt agccgccgaa
    21901 ttcagccagc ccggcataga cgccaatcat cgcgaacagg atgccaattg acgcgatctt
    21961 gacgtttctc cacggtttca tgtccaccgc aaacgcatct ttgaaggtga acggcgtgga
    22021 gcgcggtttg ataaaaccga tcaccagcat cacgaccacg ttgatgcaga acgtacaggc
    22081 cagcacatag aggaaatgga agtcgaactt caccagatag ttgatggtga tgtagctgat
    22141 tatgccaatc cccatcgcca cttttgccgc cagcgccggg atgcgcggga agaaaaagcc
    22201 cataatgatg atggtaacca gcggcacgtt gtaaatgccg ttgagctgtt tcatccagct
    22261 atacagcccc tgcggcgcgt tggcgatcca cggcgcgacc agcaccgaaa cgatagcgat
    22321 aaagaaacca aatttgcgcc cgacggtgac cagctgctgc ggctcggcat tctggttaat
    22381 gatgcgacgg taaataccca tactgaataa cgtactggcg ctattcagaa agccgttgaa
    22441 ggtactgatc accgcaccaa ataacaccgc gccgaagaaa cccaccattg gcactggcag
    22501 aacgttattg accagcgtcg ggtaggccat gtcggctttc ggtaaatcct gatacagatg
    22561 aaacgcaatc aaccctggca gcaccagtac cagcgggtcg agcattttca gcaccgccgt
    22621 taacagcgcc cccttttgcc cttccgccag gctttttgac gccagcgtgc gttgcacgat
    22681 gccctgattt gtacaccagt aaaaggtgtt caccagaatc aaaccggtaa atgccgcgcc
    22741 aatcggcaag ggatcggtcg ggccaccgat tgagtttaat ttctcggcgt gaacggtggt
    22801 gagttgctca atgccctgca taaagctgcc cttgcccatc gcgatcaggc caaataccgg
    22861 caccatcaac ccgccgataa ccagcccaat accgttgatg gagtccgcca ctgccattgc
    22921 gcgcagtccg ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa ttaccagtag
    22981 ccagatagcc gcaccgtgag aaatctgtag cgattccccg acgtgaaaca ggctgttgag
    23041 cgccaacgcg ccggagtaga gaacaatcgg cagaaagcag acgccggtgg caatgaggaa
    23101 gcagaagtcg atgataatac gcgtcgtttt atcataacgt tcctccagaa aatcggggat
    23161 ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga agatcagcgt
    23221 caccgctgaa gtcacttccc agcccatcac cgacatgccg cttttgtagg cctgcccgga
    23281 aaggccgacc agttgttccg tggaaaggtt ggttagcatt aacgaagcgg caataaccgg
    23341 cgcttttagt gaacgtccgg caagaaaata gccttgttgc gatccggtat ctattttgcg
    23401 gaccttccac caggtgatca ccgccaccag cagcgtaaaa ccgacaaaac tcaagatttg
    23461 taacgaattc atctcatagc ccttatttat gtttatgtcc cggacgcatg aatataggga
    23521 gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc gtatccctga
    23581 acggattgtt ttctgttaag ttcaggttgt gagcataatt cgccggaggg atattaaaat
    23641 gaatggaaaa ttgcaaagtt cagatgtaaa aaacgaaact ccgtacaata ttcccttatt
    23701 gattaacgaa aatgtgatct ccagcggaat ttctctgatc tcgctctggc atacctacgc
    23761 cgacgagcat taccgggtga tctggccgcg ggacaagaag aaaccgctta tcgccaactc
    23821 atgggttgcg gtgtataccg tacaaggatg tgggaaaatt cttttaaaga atggcgaaca
    23881 aataacactg catggcaact gtattatatt cttaaagcca atggatattc actcttatca
    23941 ctgtgaaggt ttagtctggg aacagtactg gatggaattt acccccacca gtatgatgga
    24001 tattcccgtt ggtcagcaaa gcgttattta taatggcgaa atttataatc aggaactcac
    24061 cgaagttgct gagttaataa cttcaccaga agcaataaaa aataatctgg cagtcgcttt
    24121 tctgacgaaa attatttatc agtggatttg tcttatgtac gcagatggta aaaaagatcc
    24181 acaacggcgg caaattgaaa aattaattgc cactttacat gccagtctgc aacaacgctg
    24241 gagcgtagct gatatggctg ccacgatccc ctgtagcgaa gcctggttgc gtcgtctgtt
    24301 tttacgctat accggcaaga cgccgaaaga atattacctc gatgcgcgtc tggatctggc
    24361 gctatcgcta ttaaagcaac aaggaaactc ggttggcgaa gtcgctgata cgctcaactt
    24421 cttcgactcc tttcatttca gcaaagcctt taaacataaa tttggttatg cgccgtcagc
    24481 cgtgctaaag aatacggacc agcacccaac ggatgccagt ccacacaatt aacgtaactc
    24541 tggccagtac gctttgttgg cctccagcaa ctcatccaga atagctttgg cgaccgaggc
    24601 gctcggtacc gttttcgaca acgtaatcgc ctaccacagg tgctgatatg aacgctgttc
    24661 ccaggcgtcc acgaccagtt tttccaccgc cacttgctga ctcattaacc ctttctgaaa
    24721 ctgcgggata tcgccgacca ccagtggttc aggccgtagt agcgcacgcg catctgtttg
    24781 cgatcttgca gcccaacaat ttgcgccatc cggctttcaa taccgattgg catgtcacag
    24841 atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac aattgctgcc
    24901 gggttggagt agttgagcat ccaggcgttt ggtgaatatt tttccatata atccaccagt
    24961 tccaggacgc cgccaatgga acgcatgccg tacgctattc cgccgggtcc gcaagtttcc
    25021 tgaccaacaa caccgtggcg cagtgggatt ttttcatcca gttcgcgcat cgggtacttg
    25081 ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga atgccacttc aggatcggtg
    25141 gtgtaactaa acgcaatgtc cggcgctttc tctttaagga tgactttaca ggcttcggca
    25201 atcacttcct gccgcgcacc atcgttatca taaaatttca gtgcacgaag cgggaaacgg
    25261 tcctgattcg ccaggagcat caacacgatg cctggcgtaa aggtgcttcc accgcctgcg
    25321 acaaccactg agaatttggt cattatactg cctccgtaat ggcaacattt tctgctgatt
    25381 gatgagaatt aattaagcta tcgagctgtt cacgcagctg ggatacatgc agaccaatga
    25441 tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt
    25501 cgtcatccag cgtttgtgac atgtcatgca gtgcaatacg taaacgcgtc gcgcaattgt
    25561 taatgctgga gatattgccg accccgccca gggcttgcag gataccggca gcctgatcca
    25621 gctctttttt tggctctgcc gcggtggttt ggcctcgcga ggctttgtat tcggcttttg
    25681 agtagagttt cacttccgca tcttcacgtc ccggcgtgca catgttgaac tgcagaatca
    25741 gtgtgcggaa aaccacgaag tacagcaggg taaagcacaa cccaatggcg atttgggtca
    25801 gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa
    25861 tcagacctcc gcccatgttg cccaccacac caaagagata cattacggtc gacattgagg
    25921 ccgccagcac ggcgtgtacc gcaaacagca acggtgaaat gaacaggaag gtaaattcca
    25981 gcggttcggt aattcccacc agcatggcgg ttaaggttgc gggaatcagc aagcccgcca
    26041 cttttacccg attttccggt gcggcagtga agtacatcgc taaagaaatg cccacggcac
    26101 caaagatttt tgagttaccg tgcagggcaa aaccgccttc cgggaacaac gatttcagcg
    26161 gctcggcgct caaactgaac tcttgcagat gctgcgccca gtacatctga atgccgcctt
    26221 caacagctgc cggaccaaag ataaactgtc cgtagatgaa gtggtgtaaa ccggttggga
    26281 tcagaatacg ttcgaggaag gtgtaaaccc agaccccaag tgcacccgcc gaacgcagga
    26341 acgcttgcag agattcaatc cccatttgta cttttggcca gccgagcaac gtcagccagg
    26401 cacaggggat catcaccagg aaggcgataa tcaccacata agacgtcccc tggaaaatgc
    26461 cgagaaaaac cggcagtttt ttatcgaaca gacggttatg cagcgccgtc acaatgccgg
    26521 aaatgataat tgcgccgata atgctggtat cgagggtttt aatcccagcc atcattgtca
    26581 gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt ccccaggtca
    26641 ttcccatcgc attgatgaaa tagttccagg tcaggaaact caccatcacc gccagacaag
    26701 cacgtccctg cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca
    26761 tattacggaa taccgtccaa ccgccctctt caataatgtg tacgatttgc gcgaataaac
    26821 tgttcggatc ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag atggcaagac
    26881 ccaccacaat cccggcgaag ggaaacagca gcactggcgt gaacatcgcg ccgccaaagc
    26941 gttgaatttg actgagcatt tccacatcct cttttctcaa ttctgtaagt aggaagcctg
    27001 attggttatg cggcaggcct ggagcgagga taggaagatg cgatcggcag aacatgacga
    27061 cccaatggga ttcgtgatcg ctttcatgct tttcgttggg cggtagagat ctacgttttc
    27121 gcgaaaaagt agacatctgt gagcactggt acacagcatc gaacacatct ttaaaaaaaa
    27181 gatgtttttt caatcgatta agcagaactt gtgggcgcat tacccgggct tgcaggcaaa
    27241 aaagagatct agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaatt
    27301 acgcggattt cacgctaaac agccttcttc ccggtgaaaa aaagttggcg gaagaatttg
    27361 cagtatcccg gatgaccatc cgtaaagcga ttgacctgct ggtagcgtgg gggctggtgg
    27421 tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtgctgcat caaaccgcca
    27481 gcctgaccgg actggtggag gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc
    27541 tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca
    27601 acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctgatgctgg
    27661 aagacagcta tatgccggtt aaactgttcc gtaatctttc gctgcaacat ctggaagggt
    27721 cgaagtttga atatattgga caagagtgcg ggattttgat tggcggtaat tatgaaagcc
    27781 tgacgccggt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc
    27841 cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg aattattcag
    27901 tgatgttcag aaatgccagc gaataccagg tggagtacca tttacggcga ctccacccgg
    27961 aaaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg ataatgcgca
    28021 gaaacatcac taacggatag acagtggcgt aagagagcgc cgccgcacca ctggttggat
    28081 gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc
    28141 acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg
    28201 gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca ttgaccaggg
    28261 tattcacaaa atccccacca gatttcagac caacgaccga gaggaacagc acgatcccca
    28321 gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg ccgatactgc
    28381 cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc agtttcaacg
    28441 cggccgggaa cccaggcaca aagacgggaa tagaacctaa caatacgcca agcccgatgc
    28501 caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat
    28561 tggcaacggc atcaattgcg gacggacgcc ccaccagatt gaggatatcg ccgaactgca
    28621 ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa
    28681 cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg
    28741 tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt ggacagcgaa gtatcgacct
    28801 cctgaccaat caccagttgc gcattatgta aatccgctgg ctgacccacc agatgcagca
    28861 aatcgcccag ttgaataatg gtatctggcg aaggaacttt tagggtttct tcgcgtttca
    28921 gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaatg gctaaatcat
    28981 gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg ccattggtgc
    29041 gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata acccgcaaca
    29101 tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg taactcatcc
    29161 ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caaaatctgc tgccctgccc
    29221 ccagcgctgg cgtattggta accgcaccgg agaaaatccc cagcactacc ggcagtggaa
    29281 tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag
    29341 caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctacct
    29401 gaatcccgat agtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat
    29461 cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca
    29521 gcacgccgcc aatacctaat cctatgccgc gaaatttgac attgccgata aacaaaccga
    29581 cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct
    29641 cttgagataa catttttaat tgctacggat tatggctgaa tcggtgaagc agatttgcct
    29701 tatagcgcac attatcacgg gaaagaacgt gccgaaatat cttaaacagt cgaaatttaa
    29761 cgcattgttg agtaagtaag acaaaaaaag ccccaccaga atggcggggc aaagagaata
    29821 gctagttagc tatttaacgc gggacgttcg ctgatagcga tacgctgcgc tgcgatgggt
    29881 tcaggctcat tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt tgcgccagag
    29941 acttccatat tttcagccag cgtaaagctc aggctaaatg gctgattcat aagcccttga
    30001 tgcagccatt ttttctcttc ttttggctgc tccggcgtgc cttttacgct caggcgcgta
    30061 ccttccagtt gaatctctaa atcttcctga cggaaacctg ccagcgcaag ggtaatgcgg
    30121 tagtggttat cgtcgctttt ctcaatgttg tacggcggga agctctggct ttcaccggcg
    30181 ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt gacgcatcag tggggataaa
    30241 tcgaagttac gcatagtcat ttctccttct aagaagcgag taagtacctg caaatccgaa
    30301 gattcgcata tgctccctga cggcgagcat ggagatgtca ggccgcgcca ggcggcctta
    30361 gggaattagt tgatttcgat acggcgcggt tttttcgctt ccggaatcac gcgttcgaga
    30421 tcgatataca gcaaaccatt taccaggtta gcaccacgaa catgaatgtt ctcagctaac
    30481 tggaatttgc gttcaaagtt gcgttcagcg atgccctggt acagataggt gcgctctttt
    30541 tgttcgtcgg cgtgagcacc tttcaccacc agcagattat cctgggcggt aatttccagt
    30601 tcgctctcag caaaaccagc cacagcgata gcaatgcggt aatggttttc gtctaccagt
    30661 tcaacgttat acggagggta gccgccatta ctctggctct ggttgttttc taagtggtta
    30721 aacaaacggt caaatccaat agcagaacgg taaagcgggg ataaatcaaa gttacgcata
    30781 atcaatagct cctgaaatca gcgagaatgt aagaccttcc acaatggaca ggtcaggtag
    30841 ccagaacacc catcaggcgc gtcctcatcg gctacgatgt aaaaatgggt ctggaaatga
    30901 acttttcaag ccttatcagc ggactttttt ttgcagttta tggtctattg catagactga
    30961 gggggcagca cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa tgatgatgtt
    31021 attttcagca agggatcgct ataactcatc aggccaacac ccacgatcaa cagatgaatt
    31081 tattatgata agaaatgtgt tgttagcgtt catgatatgc agcggaatga cattactcgg
    31141 aggatgctcc agcgtgatgt cccataccgg agggaaagaa ggaacgtatc ccggcacccg
    31201 cgccagcgca accatgattg gcgacgatga aaccaactgg ggcactaagt cgctggcgat
    31261 tctcgatatg ccgtttaccg ctgtgatgga cacgctttta ctgccgtggg atgtgttccg
    31321 caaagacagt tcggtgcgct cacgcgttga aaaaagcgaa gccaacgcgc aagccaccaa
    31381 tgcggttatt ccaccggcca gaatgcctga taactgattt agcgatcagt ttcggtgatc
    31441 cacagctggc caccttccat ccacgccagc cattgaccat ccggcgagaa gaccacggcg
    31501 tccgcagaag gtggattggc gtggttttcg gttaaatact caacctcgcc gctttgcgca
    31561 tgggcacaag caattcgatt atccagcaca aagcccaacc attctcctga cggatgccag
    31621 ttaaatgcag actgaatatc cgttttgtta tgggttaact ggcgcggctc gccgccctgt
    31681 ggcgagataa gccacagttg cacaatgccg ttatcatcac gcattaaaaa cgcgatttgc
    31741 gtaccctgcg gattacagcg cacccagtgg cgcgggacgt tgactaaccc cggataagcc
    31801 cgatggtggg taaaggttaa acgtcgctgc acgacgccgc gcggtggcgc gggcagggtg
    31861 gtttccgttc cacttaacgg cgcatcacct gccgctttcc agccagcttc atcttgcggt
    31921 aactcaacga taaacagctc cggcactttc tcgccctttg gcgaaagtgt gtcgccaata
    31981 aacgccagcg cgtgatttcc tacccatcct tcttcataag cacgattgat ttcatcgctg
    32041 ccaggctgcg gcgtgggcgt ggttttactc accagcacgc accagtggct accgctgtat
    32101 tcacgcggat gctgcttttg tacgttgacc gggccaaacg gcgcagcaac accgacgttt
    32161 cgcaaatcca gcgccggatc gagttcatgc attacatggt cgttataggt aaagctcacc
    32221 ctttcaccgt tcgggctaaa gacatgcaca tggctgccgc cgcgcagcgc tcctggcgta
    32281 tacggcgcgg taatatccat tgcatcgaga ttgctcacct tgccgccttc agcaatgact
    32341 ccgcgtcgat gatggaaatc gtaatgccat gtttcatcag gattttccgg gccatggatg
    32401 aaaacatatt tctctgactt cggatgaacg gtcaccacgc cgacgtgtgc gccctgtgac
    32461 gcgcgataga taacctcgac ctcgccggta tggatattca cacgctcaat ggtctcgccg
    32521 gtaaacgacg ccccagaagg acgcacgtca aataccagcc actggctgtc gggcgtccag
    32581 gtattggtat tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg
    32641 tcctgataca aaacgggtcc caccatcata cttcacaagg acttcacttt cagacaatag
    32701 tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt
    32761 ggcctcggcc cggcaggatc ggcgttggcg cgaaaattag caggcaaaat gcaggtgatc
    32821 gcgctggata aaaagcacca gtgtggtact gaaggtttca gcaaaccttg cggcggtctg
    32881 ctggcaccgg acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtg
    32941 atcgccaatc cacagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt
    33001 aactaccagc gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg
    33061 ctgatcccca ccagcgttga ggtttatcac gatagcctgt gccgaaaaat ctggcgtgag
    33121 gatgataaat ggcatgtcat ttttcgtgca gacggctggg agcaacatat taccgcccgc
    33181 tatctggtcg gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat
    33241 caaatccgta aatatgtcgc tatccagcag tggttcgcgg agaaacatcc ggtgccgttc
    33301 tactcctgca tctttgataa ttcgataact aactgttatt catggagtat cagcaaagac
    33361 ggttatttta tctttggcgg tgcttatcca atgaaagacg gtcagacgcg tttcacgacg
    33421 ctgaaagaga aaatgagcgc ctttcagttc cagtttggta aggcggtgaa aagcgaaaaa
    33481 tgcacggtgc tgtttccctc acgctggcag gattttgtct gcggtaagga caacgccttt
    33541 ctgattggcg aagcggcagg atttatcagc gccagttcgc tggaggggat tagctatgcg
    33601 ctggatagcg cagacattct gcgtgcggtg ttactgaagc agccagagaa gctcaatacc
    33661 gcttactggc gcgccacccg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc
    33721 cgatgcctga ccgcaccggc tttaagaaag tggattatgc gcagtggtat ggcgcatatt
    33781 ccacagttga aagattagcc aacgcgcttc acatcgccca ccagcaggat gtaagagagc
    33841 gcgccaatca acgcgacggc ggagatataa accagtgcag gtgcgaaacc gtaaccctgc
    33901 gccaggtacc ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac
    33961 acgccgccgg ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta
    34021 atcgaagcaa aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga
    34081 tcgttggtgt agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc
    34141 gttttacgcg caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca
    34201 gagagcagga cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc
    34261 agcgccgtga ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac
    34321 agtgtagaag ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc
    34381 agtttccagt ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca
    34441 tccaccagac cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc
    34501 aggcgcggcg gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca
    34561 gtgacaatga acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc
    34621 aaaaacgcca gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca
    34681 tgttccggga accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa
    34741 ataccggtta tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccctggaat
    34801 aaagtcgcca ctgaccaacc aaatatcgca ataaaataag tcacgcgatc taaaaaccaa
    34861 ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac atagcccatt
    34921 tgcgctttgg taataccgaa ctcttcctga atatgggcgg aagccacggc caggttagcg
    34981 cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag ataacgccga
    35041 cgccccggtt ttgctgcatt aacgggaata tccatagcga gcttcctcca gattttgggc
    35101 atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt gtattgctgt
    35161 tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg ctgttatctt
    35221 catgacgcca gagcggatta cgccagtccg gggcattttt actgaactca ataactttag
    35281 cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag ccgccaacca
    35341 tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg ttgtaatgaa
    35401 tgcccatact ttgttcctga agtacggcgt tataggaaac aaagtcgata tgcaggcaag
    35461 ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacatca taggcttctg
    35521 ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagagg tccggttgca
    35581 gaatcgaaat accacctgcc tccagcacgc gtttaaaatc gaaacgtgag aacatgcgct
    35641 cacccgccgc cagtggaata tgcgtttgtg ccgccagttt cgggtagtat tcggcttgtt
    35701 ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct ttaatcagca
    35761 ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca atctgattgc
    35821 caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg cgggagttat
    35881 caattagccc cagttcttca caaccgttca gtttaaaggt atcgaagccg atttcgcgca
    35941 gggttttaat gccgtcgata acatccgccg gacgatcgcc gccgacccaa ctgtaggctt
    36001 taattttgtc gcgaaccagg ccgcccatca gttgccagac cggcgcattc agcactttac
    36061 ctttgatatc ccataacgcc tggtcgatcc cggcgatggc gctcatcagg attggaccgc
    36121 cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc gatggatcct
    36181 gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg gcgcggcctt
    36241 caatcacggg ctcgccccaa ccgaccacgc cttcatcggt ttcaattttc aggaacatcc
    36301 agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg cctctcgata
    36361 cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc cggcgcgata
    36421 gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg ccaggttttc
    36481 tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg cttttaacgc
    36541 tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct gcgcgcccgc
    36601 ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg tcatgccgta
    36661 gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga gctgacagcc
    36721 catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc caatcaacgc
    36781 cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg gggaattcag
    36841 cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg ccagcgcctc
    36901 gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc actgcatgag
    36961 cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc acgtcgcaac
    37021 ccatcgcctg aaaggcttgc tggtagcgcg cggtcagcga tgttccggcg acaagggtga
    37081 tggcgtgttg atgggtcaca tagtcgcgca tactggcaac ctctgcgcca atcaacaaac
    37141 cagagagaaa ttcgctgacc tgttcgcggg gaagtgttcc cagcacatgc gaggcgcgaa
    37201 cttcaaaaag ctgcggcaat atggcgggcg cattaaggcc gcgctcaagg ccagccgcga
    37261 aggcatcggc agagttttcc tgcggcggca aacctgcgcc aatcaatgag tgatttaaca
    37321 gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc tggctatcgg
    37381 cctgcaccca tttgcaatgc gtaccgggca taacataaag agaggaagga gccagagctc
    37441 gcgcgccgat caattgtgtt tcttcgccgc gcatcacatt gtggttatcg tcatgagaga
    37501 cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt tcgccaatag
    37561 acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca ttgctgccga
    37621 ccattcctgc catcaccact ggcgtatttt cttcacgcca gtcggtcgtg acttctgcta
    37681 acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat tgcctgctct
    37741 ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc cagtcaattg
    37801 cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg cgatcatggt
    37861 aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt attgcatcga acagcgcctt
    37921 atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc gttcaaaaac
    37981 cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt tatgcaccga
    38041 ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc ggtcctgatt
    38101 gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg aagtcgcgcg
    38161 ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc tcatggcact
    38221 gataagccgt gggtcgtagt cgttttccaa cacccattgc agtacgtcag tgtcgaggta
    38281 attccactgg ttacgcggtg ccacaaacgc cccgcgataa cgtttcattt caatcagccg
    38341 cttcgccatc agcgaacgga acacctcacg gatgatgttg cgcgaggttg caaactcttc
    38401 acagagctcc gcctcagccg gaagtggcga gccaggtacg tatttgccgt gaacgatctg
    38461 tttacccagc gtaatgacaa tgcgatcggt tttattgaga gtcatggaga gtccttgtgc
    38521 tctgtatgtt cttctctact ttaccccgat cgatgcataa cgcggcaact ttgtagtacc
    38581 agcgtgatga cgttcgcgtt tgccgtgtga gtaatgtagt acaaacttat attgttgtac
    38641 tacaatttag atcacaaaaa gaacaatgca taaaaaaatg acatgcgtcg ggcagaaatc
    38701 tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc aacggcttag
    38761 ctactcgttt aaaggattaa tcatgaagtt gaattttaag ggatttttta aggctgccgg
    38821 tttattccca ctggcgctga tgctttcagg ctgtatctcg tatgctctgg tttcccatac
    38881 cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcactg ggctatcgca
    38941 ggcaaaagat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc tggattacct
    39001 tatcactgat ggtgccgatg acatcgttaa gatgctcaat gatccagcac ttaaccggca
    39061 caatattcag gttgccgatg acgcaagatt tgttttaaat gcggggaaaa agaaatttac
    39121 cggcacaata tcgctttact actactggaa taacgaagaa gaaaaggcac tggcaacgca
    39181 ttatggtttt gcctgtggtg ttcaacactg taccaggtca ctggaaaacc taaaaggcac
    39241 aatccatgag aaaaataaaa acatggatta ctcaaaggtg atggcgttct accatccatt
    39301 taaagtgcga ttttatgaat actattcacc cagaggcatt ccggatggtg tttccgcagc
    39361 attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat ttctggttgt
    39421 atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt tgcccgtcca
    39481 ctgattaatt cagcacatac ttctcaatag caaacgccac gccatcttca aggttggatt
    39541 tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc gccacaccga
    39601 cgcctgcata ttcaatcatt gcgatatcgt tttcctgatc gccaatcgcc atgatttctt
    39661 ccggtttaat acctaacacg tcggccagtg atttcacccc cgtacctttg ttaacgcgtt
    39721 tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc tctttcactt
    39781 cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc atcatcactt
    39841 tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc ggaatggtgg
    39901 caacgaagga ttcatgcacc gtgtagtagc tgatatcacg gttggcggtg tacagcgtgg
    39961 tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc aggaaacgat
    40021 agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc ttctgtacca
    40081 gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc tctttcaggt
    40141 agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg ccacgggcgc
    40201 gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc agcagaaggg
    40261 tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt agattagttc
    40321 ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg gaaaaggggg
    40381 atgcaacgcg cgttgcgtgc tccccatttg cttagcggaa gagtttccct ttcagcagtt
    40441 ccatgcctgc ggaaagcaga tcgttattgg cttgtggaga cacttcacct tgcggtgaga
    40501 gcgcatcaat aatcttcggc aattgttctg ccagtaaact ggaagctgta ctggtatcca
    40561 cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat tccagttgct
    40621 cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga atggccccta
    40681 agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc tcaacccaac
    40741 ttaaaatcgc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca acttcatcaa
    40801 aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt atagatctgc
    40861 gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg cccttttctt
    40921 atagcttctt gccagatgcg gcgctaacgc cttacccggc ctacaaaatc atgaaaattc
    40981 aatacattgc aagattctcg taggcctgat aagcgtagcg catcaggcac gctcgcatgg
    41041 ttaacgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa taaacgcacg
    41101 gcgcggttca acggcgtcgc ccatcagcgt ggtgaacagc tggtcagcag caatcgcatc
    41161 cttaacggta acgcgcagca tacgacgact ttccgggtcc atggtggttt cccacagctg
    41221 ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc gacgggactc
    41281 tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac gctcgccacg
    41341 ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg tgcagatacg
    41401 acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat ccacaccgtg
    41461 ggtacgcacg cgaacaatcg gctcgaacag gttttgctcg gcattggtgt ggacatcaaa
    41521 cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg cgttcaccca
    41581 gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct gatagataag
    41641 ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt tctgcgtcgc
    41701 gttgtactca gacaccagtt tctctaacgc ttcgccagcc aatgccggtg cactggcgtt
    41761 ggtgtgcagc gttgcgccat ccagcgcgat ggagatttgg tactgatcca tcgcttcgtc
    41821 gtctttaata tactgttcct gcttgccttt cttcactttg tacagcggcg gctgagcgat
    41881 gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg tcaacagcag
    41941 cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc tgtgataacg
    42001 cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg tgataagcgt
    42061 cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt tgaggatttt
    42121 acccttcagc ggcagaatcg cctggttctt gcggttacgc ccctgcttcg cagagccgcc
    42181 cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt cctggcagtc
    42241 tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg tcatttcacg
    42301 cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa ccacgatttt
    42361 cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct gctgttcaac
    42421 cgccgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga atttcgggtc
    42481 cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac cggtggcgct
    42541 gactttggct tttttgctgt agccttcttt gtccatgtag gcattcaggg tacgggtcat
    42601 cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt tgttggtaaa
    42661 gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga cgccaatacc
    42721 gtctttttcg gtggagaagt agaagatatt cgggtggatc ggcgttttgt tcttgttcag
    42781 atattcaacg aacgccttga tgccaccttc atagtggaag tggtcttctt tgccgtcgcg
    42841 cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac gcagacgttt
    42901 cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc tgggccagaa
    42961 acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccagcgggg cctgcggtac
    43021 accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca gctccagttt
    43081 ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg acactttata
    43141 ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt ccgccgccga
    43201 tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct gtacagagac
    43261 agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga gcgcttcgtc
    43321 gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt catccgtgtc
    43381 gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga ctttgatact
    43441 ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg ggttaatccg
    43501 ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg tctataacgt
    43561 gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag cggctggcaa
    43621 gcaacccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg tagagacacc
    43681 gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg cacatcaaca
    43741 gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg cgaatgcgta
    43801 agtccgcttt gtgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt tcaaaattac
    43861 gttccagtac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa gagaaagtca
    43921 gagaaaactc agggagaaat tgcttacagg tatcagccat atcggccgcg ataccggcgc
    43981 tgtactgggc gcgccaggtg ctgatttgct ccgccagcgg gatcagttct ttatcccacg
    44041 ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc ttgagcaatc
    44101 gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat ccccagtcga
    44161 ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct ggcgttatca
    44221 gctgcattgg catcaggtgc gccagttccg cgactttatg cccgtcagta ccgtcgatgc
    44281 ggactttgct gtcgccctgt ttgtccttgg ttaagccaat cgccgtctcg cgctcttcgc
    44341 cctgtaatcg cccatggaga acaaatgcct cctgctcatg gcgaatgacg cgaccaatct
    44401 gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc acgctggttt
    44461 tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg agatccgcgg
    44521 tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca gtctcattgg
    44581 catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc tggaaaccga
    44641 atcggtcagc atcatgcgga cgttttcgca tttcagcgcg ttcagaacat ccagcacata
    44701 gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga ggatctcttc
    44761 cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc tgacatacag
    44821 gcgcacgccg cggaatttct cgttagagag aattgccgca cgggcaaacg cctgcttgag
    44881 cagatcgcag ccagcttcca gatgtttgtc cggattcttc ggcagaacgc ggcggtaatc
    44941 cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt gggcgcgaat
    45001 attgttgctg ccaatctgca cgcgcagcgg attgtcgccg ccgtcgagca tacgcatcag
    45061 ttcaatcacg cctttacgcg gtacgatcac cgaatggctt ggcaaagatt gaccaattgg
    45121 cattgaacag accgccagac ggtggccgtc ggttgccacg gtgcgcagtt cttcaccttc
    45181 ggtttcaaac agcataccat ttaagtaata gcgaacgtcc tgatgcgcca tcgaaaactg
    45241 ggtcgcttca atcagacgct tcatcgttgc ctgcggcagg gtaaattcga cttcactctg
    45301 ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa aacggctacg
    45361 cccggagcgt accagcatcc gttcaccttc cagctgcacg gcaatttccg cgccttcagg
    45421 cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc ctggctcgtg
    45481 tggctgaacc agcgcaacac gtgccaccat ttccatctcg agatcagtac cggtcagcga
    45541 caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg taggacgacc
    45601 acctaacgga ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt ctacggtaaa
    45661 tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct ttgatatcgt
    45721 ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc gtgtggtcac
    45781 gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct ttcgccagcg
    45841 ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa aggagatccg
    45901 cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg gtgaccagtt
    45961 tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac gaagtcgatg gtgatcgccc
    46021 gtccggtaaa gttggcattg gcaatgacgc ggttcagcgc cccttccagc tcacgtacgt
    46081 tagatcgtag acgcttggcg ataaagaacg ccacttcgcc cggcaaacga atgtcgtttt
    46141 cgtcggcctt tttcatcagg atcgccacac gggtttccag ctctggcggt tcgatcgcca
    46201 cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg atctctttcg
    46261 gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg ttgaaggtat
    46321 ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg tcgatcagca
    46381 gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt tgcagggctt
    46441 taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca ttcggcttgc
    46501 gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga cccgtgccgc
    46561 cataaaggaa caacgggtta taggcaccgc cagggttatc cgccacctgg cgagccgccg
    46621 cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt ttgacgttta
    46681 cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct gagcgcgtag
    46741 aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg acgttgctcg
    46801 tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa cgcagctgtg
    46861 gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg tacttgtccc
    46921 gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta tcgctcagtt
    46981 ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca tcctgcaatc
    47041 gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga acaaaagtcg
    47101 ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga ccctgcttga
    47161 gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg caaaacgatc
    47221 gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac agattttccc
    47281 gatttatcca caggactttc cagaactcgc taagtgtaaa cgatcctgcc gcgaggcggg
    47341 cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg gcttaatcga
    47401 tctaataaag atccaggacg atccttgcgc tttacccatc agcccgtata atcctccacc
    47461 cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca ttttccctgc gaaaaggtgc
    47521 ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt attacaatcc
    47581 ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc ggatatccaa
    47641 taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca cttttcaacc
    47701 gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta ctaaaaatgg
    47761 tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg tttctaagta
    47821 ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc ttgttaactc
    47881 ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg caaattacca
    47941 ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc gccaagaaaa
    48001 acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa agcttccgtc
    48061 tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa ggggttgccg
    48121 acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc cactgtcgcc
    48181 tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta gtccgctact
    48241 cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg cattgcgcag
    48301 gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat gccacccttt
    48361 acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag aacactaacg
    48421 atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt catgatctgg
    48481 caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac gcagacaacg
    48541 accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca ggggaaactg
    48601 atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg tgatgttgag
    48661 caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt ccagctgctg
    48721 gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg tgatggcccg
    48781 gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc ttatgtgctg
    48841 gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc aggcaacacg
    48901 tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa ctacaacgtg
    48961 cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa gcaatccatc
    49021 actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac cttccgcggc
    49081 gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac cattgccgat
    49141 aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg cgatgctgca acagtatttc
    49201 gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc taatctgggt
    49261 aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc tggtcagact
    49321 ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat ggcagctgtt
    49381 gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc tcagccgctg
    49441 ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc cattatcatc
    49501 atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta cacctccatg
    49561 gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct gggcgatgac
    49621 aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt taacccgctg
    49681 ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta ctacatgctg
    49741 atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga cctgtcggca
    49801 caggacccgt actacatctt gccgatcctg atgggcgtaa cgatgttctt cattcagaag
    49861 atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt tatgccggtc
    49921 atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta tatcgtcagc
    49981 aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa acgtggcctg
    50041 catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata agggcggtca
    50101 gttgaccgcc ttttttcttt tcgtagggcg gataagcacc gcgcatccgc cacacaaagc
    50161 aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc tccgggacgt
    50221 ggcggcgttg gcatcctgcg catctccggc ttcaaagccc gtgaagttgc cgaaaccgtg
    50281 ctgggtaaac tacctaagcc gcgctacgcc gattatcttc cgtttaaaga cgccgacggc
    50341 agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt caccggcgaa
    50401 gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc tcgacctgct gttaaaacgc
    50461 attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga acgcgcgttt
    50521 cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat cgacgccagt
    50581 tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc cgcacgggtc
    50641 aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc aattgatttc
    50701 cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct taatgacgtg
    50761 attgccgaac tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt gcgcgaaggg
    50821 atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt aaacgcgctg
    50881 gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga cgtgctgcgt
    50941 gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg gctacgtgaa
    51001 gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat tgaacaggcc
    51061 gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc ggcagagatc
    51121 tggccggaat ttattgcccg tctgccagcg aaactgccga tcaccgtggt gcgcaataaa
    51181 gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc gttaattcgt
    51241 ctctcggcaa ggactggtga aggcgtggac gtgctgcgta accatctcaa acagagcatg
    51301 ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct acaggcgctg
    51361 gaacaggcag cggaacatct acaacagggc aaagcgcaac tgttgggagc ctgggcaggt
    51421 gaactgctgg cggaagagtt gcgtctggca cagcagaact taagcgaaat caccggggaa
    51481 tttacttcag acgacctgct ggggcggatt ttctccagct tctgtattgg taagtaaccg
    51541 cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt agcactcctg
    51601 ttattcctca accctttttt taaacattaa aattcttacg ta
//
LOCUS       DAZFWP020000012        49748 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFWP020000012 DAZFWP020000000
VERSION     DAZFWP020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.012"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            182..808
                     /locus_tag="ACHK0Z_RS03325"
                     /old_locus_tag="pgaptmp_000665"
     CDS             182..808
                     /locus_tag="ACHK0Z_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU4500733.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..2931
                     /locus_tag="ACHK0Z_RS03330"
                     /old_locus_tag="pgaptmp_000666"
     CDS             829..2931
                     /locus_tag="ACHK0Z_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU4500734.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYLLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTHETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2944..3582
                     /locus_tag="ACHK0Z_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             2944..3582
                     /locus_tag="ACHK0Z_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU4500735.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3646..4314
                     /locus_tag="ACHK0Z_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             3646..4314
                     /locus_tag="ACHK0Z_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4500736.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4311..5096
                     /gene="phsC"
                     /locus_tag="ACHK0Z_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             4311..5096
                     /gene="phsC"
                     /locus_tag="ACHK0Z_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU4500737.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5100..5912
                     /gene="ydhT"
                     /locus_tag="ACHK0Z_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             5100..5912
                     /gene="ydhT"
                     /locus_tag="ACHK0Z_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU4500738.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5924..7528)
                     /locus_tag="ACHK0Z_RS03355"
                     /old_locus_tag="pgaptmp_000671"
     CDS             complement(5924..7528)
                     /locus_tag="ACHK0Z_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU4500739.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7654..7959)
                     /locus_tag="ACHK0Z_RS03360"
                     /old_locus_tag="pgaptmp_000672"
     CDS             complement(7654..7959)
                     /locus_tag="ACHK0Z_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU4500740.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8067..8143)
                     /locus_tag="ACHK0Z_RS03365"
                     /old_locus_tag="pgaptmp_000673"
     tRNA            complement(8067..8143)
                     /locus_tag="ACHK0Z_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8107..8109),aa:Val,seq:gac)
     gene            complement(8148..8224)
                     /locus_tag="ACHK0Z_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     tRNA            complement(8148..8224)
                     /locus_tag="ACHK0Z_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8188..8190),aa:Val,seq:gac)
     gene            8532..9788
                     /locus_tag="ACHK0Z_RS03375"
                     /old_locus_tag="pgaptmp_000675"
     CDS             8532..9788
                     /locus_tag="ACHK0Z_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4500741.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="ACHK0Z_RS03380"
                     /old_locus_tag="pgaptmp_000676"
     CDS             complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="ACHK0Z_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU4500742.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11417..12058
                     /gene="ribE"
                     /locus_tag="ACHK0Z_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             11417..12058
                     /gene="ribE"
                     /locus_tag="ACHK0Z_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU4500743.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="ACHK0Z_RS03390"
                     /old_locus_tag="pgaptmp_000678"
     CDS             complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="ACHK0Z_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU4500744.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13537..14748)
                     /gene="punC"
                     /locus_tag="ACHK0Z_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             complement(13537..14748)
                     /gene="punC"
                     /locus_tag="ACHK0Z_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU4500745.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14861..15793
                     /gene="punR"
                     /locus_tag="ACHK0Z_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             14861..15793
                     /gene="punR"
                     /locus_tag="ACHK0Z_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU4500746.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMNGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15790..16815)
                     /gene="purR"
                     /locus_tag="ACHK0Z_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             complement(15790..16815)
                     /gene="purR"
                     /locus_tag="ACHK0Z_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU4500747.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17114..17203
                     /gene="cydH"
                     /locus_tag="ACHK0Z_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /gene_synonym="ynhF"
     CDS             17114..17203
                     /gene="cydH"
                     /locus_tag="ACHK0Z_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU4500748.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17369..18538
                     /locus_tag="ACHK0Z_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             17369..18538
                     /locus_tag="ACHK0Z_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4500749.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18684..19265)
                     /gene="sodB"
                     /locus_tag="ACHK0Z_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             complement(18684..19265)
                     /gene="sodB"
                     /locus_tag="ACHK0Z_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU4500750.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19393..20208)
                     /gene="mepH"
                     /locus_tag="ACHK0Z_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             complement(19393..20208)
                     /gene="mepH"
                     /locus_tag="ACHK0Z_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU4500751.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTTSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20543..20890
                     /gene="grxD"
                     /locus_tag="ACHK0Z_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             20543..20890
                     /gene="grxD"
                     /locus_tag="ACHK0Z_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU4500752.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20941..25557)
                     /locus_tag="ACHK0Z_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             complement(20941..25557)
                     /locus_tag="ACHK0Z_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU4500753.1"
                     /translation="MADNPDSSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHQARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25650..26297)
                     /gene="rnt"
                     /locus_tag="ACHK0Z_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             complement(25650..26297)
                     /gene="rnt"
                     /locus_tag="ACHK0Z_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU4500754.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26400..26807)
                     /gene="gloA"
                     /locus_tag="ACHK0Z_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             complement(26400..26807)
                     /gene="gloA"
                     /locus_tag="ACHK0Z_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU4500755.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26888..27985)
                     /gene="nemA"
                     /locus_tag="ACHK0Z_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             complement(26888..27985)
                     /gene="nemA"
                     /locus_tag="ACHK0Z_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU4500756.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIENPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28022..28621)
                     /gene="nemR"
                     /locus_tag="ACHK0Z_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             complement(28022..28621)
                     /gene="nemR"
                     /locus_tag="ACHK0Z_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU4500757.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28724..28963
                     /locus_tag="ACHK0Z_RS03460"
                     /old_locus_tag="pgaptmp_000692"
     CDS             28724..28963
                     /locus_tag="ACHK0Z_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU4500758.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29012..29908
                     /locus_tag="ACHK0Z_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             29012..29908
                     /locus_tag="ACHK0Z_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGU4500759.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29989..30510
                     /gene="sodC"
                     /locus_tag="ACHK0Z_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             29989..30510
                     /gene="sodC"
                     /locus_tag="ACHK0Z_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4500760.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLTALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30511..32523)
                     /locus_tag="ACHK0Z_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             complement(30511..32523)
                     /locus_tag="ACHK0Z_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4500761.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDVGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKSQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32523..33380)
                     /locus_tag="ACHK0Z_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             complement(32523..33380)
                     /locus_tag="ACHK0Z_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4500762.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33383..33619)
                     /locus_tag="ACHK0Z_RS03485"
                     /old_locus_tag="pgaptmp_000697"
     CDS             complement(33383..33619)
                     /locus_tag="ACHK0Z_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU4500763.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33820..34254
                     /gene="slyA"
                     /locus_tag="ACHK0Z_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             33820..34254
                     /gene="slyA"
                     /locus_tag="ACHK0Z_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU4500764.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34301..34768)
                     /gene="slyB"
                     /locus_tag="ACHK0Z_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             complement(34301..34768)
                     /gene="slyB"
                     /locus_tag="ACHK0Z_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU4500765.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35042..36151
                     /gene="anmK"
                     /locus_tag="ACHK0Z_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             35042..36151
                     /gene="anmK"
                     /locus_tag="ACHK0Z_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU4500766.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36249..36578
                     /gene="mliC"
                     /locus_tag="ACHK0Z_RS03505"
                     /old_locus_tag="pgaptmp_000701"
     CDS             36249..36578
                     /gene="mliC"
                     /locus_tag="ACHK0Z_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU4500767.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36637..37293
                     /gene="pdxH"
                     /locus_tag="ACHK0Z_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     CDS             36637..37293
                     /gene="pdxH"
                     /locus_tag="ACHK0Z_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU4500768.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQLENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37422..38696
                     /gene="tyrS"
                     /locus_tag="ACHK0Z_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     CDS             37422..38696
                     /gene="tyrS"
                     /locus_tag="ACHK0Z_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU4500769.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38755..39618
                     /gene="pdxY"
                     /locus_tag="ACHK0Z_RS03520"
                     /old_locus_tag="pgaptmp_000704"
     CDS             38755..39618
                     /gene="pdxY"
                     /locus_tag="ACHK0Z_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGU4500770.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39662..40267)
                     /gene="gstA"
                     /locus_tag="ACHK0Z_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             complement(39662..40267)
                     /gene="gstA"
                     /locus_tag="ACHK0Z_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU4500771.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPAVLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40373..41875)
                     /gene="dtpA"
                     /locus_tag="ACHK0Z_RS03530"
                     /old_locus_tag="pgaptmp_000706"
     CDS             complement(40373..41875)
                     /gene="dtpA"
                     /locus_tag="ACHK0Z_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU4500772.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42486..43121)
                     /gene="nth"
                     /locus_tag="ACHK0Z_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             complement(42486..43121)
                     /gene="nth"
                     /locus_tag="ACHK0Z_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU4500773.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43121..43816)
                     /gene="rsxE"
                     /locus_tag="ACHK0Z_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             complement(43121..43816)
                     /gene="rsxE"
                     /locus_tag="ACHK0Z_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU4500774.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43820..44440)
                     /gene="rsxG"
                     /locus_tag="ACHK0Z_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             complement(43820..44440)
                     /gene="rsxG"
                     /locus_tag="ACHK0Z_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU4500775.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44444..45502)
                     /gene="rsxD"
                     /locus_tag="ACHK0Z_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             complement(44444..45502)
                     /gene="rsxD"
                     /locus_tag="ACHK0Z_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU4500776.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45503..47725)
                     /gene="rsxC"
                     /locus_tag="ACHK0Z_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             complement(45503..47725)
                     /gene="rsxC"
                     /locus_tag="ACHK0Z_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU4500777.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQSNAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(47718..48296)
                     /gene="rsxB"
                     /locus_tag="ACHK0Z_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     CDS             complement(47718..48296)
                     /gene="rsxB"
                     /locus_tag="ACHK0Z_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU4500778.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48296..48877)
                     /gene="rsxA"
                     /locus_tag="ACHK0Z_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     CDS             complement(48296..48877)
                     /gene="rsxA"
                     /locus_tag="ACHK0Z_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU4500779.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48954..49394)
                     /locus_tag="ACHK0Z_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     CDS             complement(48954..49394)
                     /locus_tag="ACHK0Z_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4500780.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49480..49695)
                     /gene="ydgT"
                     /locus_tag="ACHK0Z_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     CDS             complement(49480..49695)
                     /gene="ydgT"
                     /locus_tag="ACHK0Z_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU4500781.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT        12504 a        12390 c        12967 g        11887 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat
       61 acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg
      121 aatgcttaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt
      361 gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccacc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc
      541 ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag
      661 cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt
     1081 accaaaggaa atttagttgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa
     1141 ttcgctggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc
     1261 gcaacgacgg aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc
     1381 ggtgcgggaa ctggcgcaat tatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg
     1501 actgaactta ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca
     1561 gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggtggtc cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg
     1741 agtggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag ccaactttgt ccataccacc
     1861 atcttcccga acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac
     1921 ctggttggtc tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagctgcat
     1981 cgcgacttta cttattgtta cagcaaaggg gtgttcaagc gtgttctgcc agctgaagag
     2041 tatgcggaaa ttcactggga tcaactggaa gcgggtgacg ttaatttcat taaagatttt
     2101 tactaccttc tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc
     2161 atcgcagaac gctggaattt gggtgaagag tactggggct acgcaaaaaa taaactctgg
     2221 tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt
     2281 gttaactgta tgttcaaccg tgactgcatg acgcataccc atatcaattt tattggttcg
     2341 ggtttgccat tgaaactgca gcgtgaagtg gcgaaagagc tttttggttc tgaagatgcc
     2401 tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgcaaagtgg
     2461 tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg
     2521 accgtttcgc cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc
     2581 ttcaaagcga tcaccggcga agagatgact caggaaaaat tagatttagc tgcagagcgt
     2641 atctttacgt tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat
     2701 gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact
     2761 gaaggtactg acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa
     2821 gaaatgggct gggacccaca gcttggttgt ccgacccacg aaacattgca gcgtctgggg
     2881 ctggaagata ttgccgccga tctggcagca cacaatctac tgcctgcgta aggaatgggt
     2941 gagatgaacc atcgggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag
     3001 cggcaatggt tacaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct
     3061 gaaatcctgg cagagttcat tcgccagcat tctgtggctg ggcagctggt ggcgcgcgcc
     3121 gtgtttctct cgccacccta ttcggttgca gaagaggaac tgtcagtttt gctggaaagc
     3181 attaagcaga acggagacta cgcggatatc gcttgtatga cgggaagtca ggatgattac
     3241 tattactcaa cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag
     3301 caggacattt gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt
     3361 ccttacaaag tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa
     3421 gccgcgatcg ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca
     3481 tcgacggcgg tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc
     3541 ttatgcgaat ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca
     3601 tggatagggt ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga
     3661 cgcaaatttg ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg
     3721 ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg
     3781 tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag
     3841 ggaagccgtt tatcgatagc acacattcca gtcaccgata acgataacga aacgcagtat
     3901 cacttttttc gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg
     3961 acaggggcgt cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc
     4021 ggctgcagtt attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact
     4081 aaagtggcag ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc
     4141 atttgcgtca gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa
     4201 attcaggcgt ggttgcagca aaataaatac tatcaatatc agctacctgg tgccgggaaa
     4261 ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt
     4321 cgcaacatgc tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg
     4381 aattttggcc ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc
     4441 tggggctgca tgccttgctt cgtgctcgtg gcgtgaagaa atcagtcacc gattatggtg
     4501 agaagattta tctttactgc aaagcggtca gattatggca ctggtcgaat gcgttactct
     4561 ttgtattgtt gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactgcgg
     4621 ttaaaagtct ggttgcggtg catgaagttt gcggattttt gttactcgca tgctggctcg
     4681 gctttgtgct gatcaatgcc gttgggggta atggtcacca ctatcgcatt cgtcgtcagg
     4741 ggtggctgga gcgagcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg
     4801 aagaacatcc tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct
     4861 atgttggtgt tatgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct
     4921 atccgcaagc cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt
     4981 ttgctctggc atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg
     5041 ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa
     5101 tgatcattac acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt
     5161 tcctgcgcca tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg
     5221 aaggatatac ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg
     5281 atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag
     5341 agataccatt ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg
     5401 aggataacgc ccgaatcgca accgctgggt atgccgcgca aattgccagt atgggttata
     5461 gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac
     5521 gggttgctgt aaccggtaat tcttcgcgga taagcagcgc tggagatagt agtcggattg
     5581 cgaataccgg aatgcgagta cgcgtttgta cattgggtga acggtgtcat gtcgccagta
     5641 atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg
     5701 acaatgtaca catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg
     5761 actcaattat tctcgggcca gggggaagcg ccgcactggc atatcatgac ggcgaaagag
     5821 ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg aaccggagtc cgctatcgtc
     5881 ttaatgaaca gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac
     5941 gccgacgagc acgggatgca ggctttacga ccgcccgagc catcgcctca ccaatttctg
     6001 cacatgccgt aagtccctga acgaaaggct ggtcgtgcat caaccagggc aacgcgccga
     6061 acgctacgcg tccacgaata tcttcgggtt gctgtaacgt ataatcttcg ccaacatcag
     6121 ggatttcatc ccctgttttc tgtaattgct cgcgtagccc agggaaagga atatctttca
     6181 ctttaagcgg acgctgtccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt
     6241 ccgttttcag gacggtgcgc gactcattaa tttccatttt gtagtcttca ccgagggcga
     6301 gaatatgaat gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga
     6361 ttgccgcata attatcgatg aaaaccctgg caaggccttt actgaaccgt ttatggtcgt
     6421 gttcattcag atgaggaaca atttcctgta cggcttcatg caggcgcaga atgacataac
     6481 gccagggaac agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg
     6541 cccagtcaaa tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga
     6601 cattcaggct ctctaaggct atgcgttggc tccagtctgg atcagcaaac ttgatttcct
     6661 ctactatcaa tctaaatacc cgatccaaaa ggccctcttc acctttttga atctcagcat
     6721 ttaatgcctg atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg
     6781 cttcgggtaa aatacccgtg cgcgacatca acgtaatatt tagcttttca cttgcgttat
     6841 cgcggttaaa aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca
     6901 ctgccattgc cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat
     6961 cgacctttgc ttccatcagg cctgaccacg gactgggaaa atacgttctg gttgcttctt
     7021 cttcatcagg ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta
     7081 aatcctgatt tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc
     7141 atgattcata aacagccact gcaaattttt gctgtcgtgc ctggtctact aatcgtaaaa
     7201 attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg
     7261 tttctttttt aacgccataa cgctggagat ggctggcttc ttgcttttgt agccattcga
     7321 gatacgtaca attaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg
     7381 agttttcctc atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag
     7441 aaatagaaag tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc
     7501 cggcacccac aatagcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt
     7561 agtgttatca gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg
     7621 aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg
     7681 actcagtggc tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagattttt
     7741 aagtcgggca gtatgttttt ccagataggc aagggcgctt ttttcatcag taaaaagata
     7801 gatcccaccg gcttcgtggt tcttttcact ttctgtccat accttccaca gaaaaccagg
     7861 ttcctgatta atcgactcag caagcggttt aagctgctca gccattgcgt cgccaaatgg
     7921 gccattaaaa gcaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac
     7981 gcatacgaag acagtgtaaa tcttttaacg tattggatac agagaagaaa cagagggaaa
     8041 aaggagaaga ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc
     8101 caccatgtca aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat
     8161 tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga
     8221 acgcaacgtt gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag
     8281 aggcaaaacg taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc
     8341 tatattttgt tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa
     8401 cccaataatt cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg
     8461 tggataaatc ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa
     8521 aggaataaat catgggatct gatgcgaaaa acttgatgag cgacgggaat gtgcaaattg
     8581 ttaagaccgg cgaggtcatt ggcgcgacgc aacttactga aggcgagttg attgttgaag
     8641 ctggcggaag agccgaaaat accgtggtca cgggggctgg ctggttgaaa gtggcaaccg
     8701 gtgggatcgc caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg
     8761 ccattgccac agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta
     8821 cggttaatgg ccgccatccc gaaggtgaat tcagcgttga tcagggttat gcctgcggtt
     8881 tgttgctgga aaatggcggt aacctgcgtg tactggaagg acatcgcgcg gaaaaaatca
     8941 ttctcgatca agagggcggc ctgttggtta atgggacaac ctcagcggtc gtggtagatg
     9001 aaggtggtga attgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg
     9061 gcggcgtttt tatgctggcc gggaaagcca gtgatacgtt gcttgctggt ggcaccatga
     9121 ataatctcgg tggtgaagac tctgacacta ttgttgagaa tggatccatt tatcgtctgg
     9181 ggacggatgg ccttcagctc tacagttccg gtaagacgca aaacctgtcc gtgaatgtgg
     9241 gtggtcgggc tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag
     9301 tgatcctgtt gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac
     9361 cggttgaact gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggctatg
     9421 gcgcagatct gcaacaatca acgattactg tacagcaggg cggtgtgttg attctcgacg
     9481 gcagtacggt aaaaggtgac ggtgtcactt ttattgttgg taacatcaat ctgaatggcg
     9541 gaaaactgtg gctgatcact ggtgcggcaa cgcatgtgca actgaaagtg aaacgcctgc
     9601 gcggagaggg agcgatttgc ctgcaaacca gtgcgaaaga aatctcacct gacttcatca
     9661 atgtgaaagg ggaagttacc ggggatatac acgttgagat aacagatgcc agtcggcaaa
     9721 ctctgtgcaa cgctctgaaa ttacagccag acgaagacgg gattggcgca acgctccagc
     9781 ctgcgtaaaa acacaggcgc aacccgtgaa ggttgcgcct tgtctttatt agcgggatgc
     9841 tcgttgcaga atgataactg acggcagacg ttgcaggaac cgcatacgca gcatcatcat
     9901 aatggctgcc gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg
     9961 ttcaacgacc agatcggtca gtgccagaat atagccgctt ggcaagccca gcacccagta
    10021 agccgtaaag gtgatataga aaatggaacg cgtatcttta taaccacgca aaatcccact
    10081 gccaatcacc tggattgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc
    10141 agccagcgtt acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga
    10201 aaccgtgaat atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc
    10261 agcggtttgc gcatccagcg ttgagccctg acccagacga taacctacgc ggatagttac
    10321 cgctgccgcc agcgacattg gaagcacaaa catcagtgaa ctaaagttca gggcaatctg
    10381 gtgtcctgcg acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag
    10441 tgtcacttca aagaatagtg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat
    10501 aaccgcagga tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tggagcgggc
    10561 gcgtttaatg taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac
    10621 gccacaacca acgccaccga gctcaggcat accgaaatga ccataaataa agatatagtt
    10681 caccggaatg ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc
    10741 cagaccttca cattggttac gggcaacctg gaagaacaga tagcccggcg cgccccacaa
    10801 caacgcacgc agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga
    10861 gcggataatg taacctgcat tccacagcac aagcataatg agaacggaaa caaaacctgc
    10921 cagccagaaa ccttgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa
    10981 ttgcgcgata accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag
    11041 ccagatagaa gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat
    11101 cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc
    11161 taatgctaat aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg
    11221 tagttatatg agagactaaa aagccgcctg aatgggcagc aaaaagaaga agcaggggaa
    11281 attcagtcta ttgtagcgag gatttattaa ttctccagtg aaaaaatagt cattcttacc
    11341 ccttcactgg taagggcgtt tgccacctgc tattgtgctg aacagaatgt cttaactgat
    11401 ttcaggagtt gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat
    11461 tgacgagaaa ccaaattttc gtacgcatgt ggtggagtta cccgaccaca tgctggacgg
    11521 cctggaaaca ggtgcttccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa
    11581 cggcaaccat gtcagttttg acctgatgaa agaaacgtta cgcattacca atcttggcga
    11641 tttaaaagtg ggggattggg taaacgttga gcgtgcggcg aaattcagtg atgaaattgg
    11701 cggacactta atgtcaggtc atattatgac caccgctgaa gtggcgaaaa tattaacctc
    11761 ggaaaataat cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatatcct
    11821 gtacaaagga tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac
    11881 gcgtttttgc gtccatttaa ttccggaaac tctggaacgc acgactcttg ggaagaaaaa
    11941 actcggcgca cgcgtcaaca ttgaaattga tccacaaact caggcagtgg tagatacggt
    12001 agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa ccaggtacag aagcctgatc
    12061 tgaaatcgcc cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt
    12121 tttcaacacc gcgtgagaac acgacctgcc agagttgaat atcacgggcg cggaaagcac
    12181 ctgcacaggc attcagataa taggtaaaca ttcgtttaaa gcgttcactg tagttatccg
    12241 caatttctgg ccatgcggcg aggaatcgtt cataccacgc catcaacgta gtatcgtaat
    12301 cagcaccgaa gttatgccag tcttccatca cgaagtgggg ttcgctggat tgagcaatct
    12361 ggcgtacaga gggcaggcaa ccgttcggaa aaatatattt attaatccag ggatcaacat
    12421 tcagatcggt ttttttcgaa ccgatagtat ggagcaggaa tataccttcc ggtttcaaat
    12481 tacgatccac caccgcaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc
    12541 ccacagaaac aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg
    12601 tgacatccag gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga
    12661 cgccgaccac gcttacgtca tagttagatg ccatgtagtg cgccagtccg ccccagccgc
    12721 agccaatatc cagtacgcgc atccctggtt ttaactgcaa tttttcacaa atcattttga
    12781 gcttcgcctg ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt
    12841 gcatgaaggg atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctccttgc
    12901 cgactatcca ggcacgtttt ttactctgca gattgaagag acgagcgctg gcaatacgca
    12961 gcgtgtcttt gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa
    13021 aaaacatatc cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca
    13081 aagagccttc ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg
    13141 gggcagaacc gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc
    13201 agttgtcatc cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac
    13261 tggtcggtga tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc
    13321 gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacctttgc
    13381 cgaaatgacc atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac ataatattta
    13441 tcgtagcttt aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt
    13501 ttgttagcag cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac
    13561 ttcggcattg ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag
    13621 agcgaccagc actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat
    13681 actgatcagc caggaaacta ccagacttgc gaggaagcac agacccagtt gtagagtgtt
    13741 ctgcaacgct gcggcgcgac cagttgcgtg tgggaaggga cgcagcgcct gggcgacaac
    13801 aatcgggtag atcgcgccat tggcaatcgc catcacacag aatgggatca ggatttcgac
    13861 cagcgacaca tggctaatga agcctgcagc ccaggtcgca atgacgctga ctgcaaacag
    13921 caccagcaac cacggtaata actgcttgcc ttgccatttc tgcagcgcgg cgcgacagcc
    13981 ataaccacca atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg
    14041 gctgtagccc atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc
    14101 tgaacaggcc gcgtatatca gcacgttgcc gcgataggtt ttagaacgta gcaggtcggt
    14161 aaaggtcaga ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat
    14221 aggcagaatc agcaccacgg taatggcaaa cagggtggcg aaaatcgcct gccaggaaaa
    14281 atggaccagc agccagcttc ctaacagagg agccagggcc ggagatagac ccaccagcgg
    14341 catgatggtc gcaaaaattc ggttaacttt ctgtgaagga taataatccg tcactaacgc
    14401 ttgccagata accgccgcgg cgcagacacc cacagcctgt acaaaacgca ataccagcag
    14461 cgtagcggcg ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc
    14521 gattaataac accggtttac gaccataacg gtcggagagt ggcccccaca gaagctgggc
    14581 tgcggcaaaa ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg
    14641 caggtcggcc tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag
    14701 tacgctcaaa cccgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta
    14761 aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt
    14821 aatatttgat gactggtttc aaaaattttg caggcagact atgtggtcag aatattcact
    14881 cgaagttgtt gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagctgca
    14941 tcgcgttcct tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtgcc
    15001 gctctttgaa cgacggcacc gtgatgtgga gctgaccgct gctggagcgt ggtttctcaa
    15061 agaagggcgc tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc
    15121 gaacggctgg cgcggtcagt tagctatcgc agtggataat attgtcaggc cagaacgtac
    15181 acggcagatg atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca
    15241 ggaagtgttc aacggtgtct gggatgcgct ttccgacggg cgcgtggaac tggcgattgg
    15301 cgcaacacgc gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag
    15361 ctggagttgc gttgttgcca gccaccaccc gctggcgttg atgaatggcc cattcagcga
    15421 tgatacgttg cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa
    15481 acgtattacc tggttgctgg ataaccaaaa aagagttgtc gtgcctgact gggaatcatc
    15541 ggcaacctgt atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc
    15601 gtggcttaat gaggggaagt gggtagcact tgaattggaa aaccccttcc cggattcggc
    15661 atgttgtctg acatggcagc aaaatgatat gtcgcctgcg ttaacctggc tgcttgagta
    15721 tctcggcgat agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc
    15781 aacgggtgat taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat
    15841 caagcgcgga tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga tacgatccaa
    15901 cagcatgttg aacgctgttt cacccagcga atcttttggc tggtggatcg tggtcagcgc
    15961 cggcgtaaaa tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg
    16021 gacgcgcaga cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc
    16081 acagaagacg gcagtagggc gatgcggctg cgacagtatt tgctgcatgg cgcgataacc
    16141 agattcgggt tcaaagtcac cctgaacaat ccagctttcc ggcaccttga tcatcgcttc
    16201 ttccatcgcc ttcataaaac cggcaaggcg gcctgcgccg gtgttacgtt ccagcgggcc
    16261 ggggatgacg ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc
    16321 gccttcgaac gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc
    16381 catcaccacc attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta
    16441 ctcagaacac atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc
    16501 ccgctgtttc tcaagattgt tccacgcatt acccagaatc agggtgtaac ctttctggaa
    16561 gcaatttttt tcaaccgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc
    16621 cagcaaaccg atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga
    16681 gtagtgtaat tctttaatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg
    16741 tgttttgttg attacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc
    16801 ttttattgtt gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa
    16861 ggtaaacgtt tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa
    16921 acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat
    16981 tttggccgat cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg
    17041 agattttttc atcacagtgt gtaaaaatgc gtaatatcac tatttgtgtg gtaataaaaa
    17101 aggagaaaac ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct
    17161 cggtttgatc gtggccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc
    17221 gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt
    17281 ttgttatttt catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg
    17341 taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc
    17401 gattggcgcg tttggtatcg ggacaacgga gttctcgcca atgggcttgt tgcccgtcat
    17461 tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt
    17521 tggcgtaatg gttggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag
    17581 tgcgttgatt ttcctgatgg caattttcac gctcggcaac gtactttccg ccatcgcgcc
    17641 ggattatatg accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt
    17701 tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt
    17761 tgccactatg tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg
    17821 gttgggtgaa accatcggct ggcggatgtc atttctggca acggcggggc tgggagtgat
    17881 ttcaatggta agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt
    17941 gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc tgacgacggt
    18001 actgggagct ggtgcaatgt ttactctcta cacctatatc tctccggtac tacaaagtat
    18061 tacccacgca acaccagtgt tcgtcacggc aatgctggtg ctgattggtg tcggattctc
    18121 tatcggtaac tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg
    18181 ctttttgttg ctgctgatgg tgattatgct ggcaatcccg ttcctggccc gcaatgagtt
    18241 cggcgcagct attagcatgg tggtgtgggg cgcagcaacc tttgcggtcg taccgccgtt
    18301 acagatgcgc gtgatgcgtg tcgccagtga agcgccgggt ctgtcttcat cagtcaatat
    18361 tggtgccttt aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc
    18421 tgggctggga tacagctttg tgccggtgat gggagcgatt gtcgcgggac tggcattatt
    18481 gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt
    18541 aaacaaaaac gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc
    18601 ttgccgaatg cgctgttaac gcctcatccg cgttggcatg gtaagcgaag cgcatcaggc
    18661 aatgctgcat ttgccatcag ttattatgca gcgagatttt tcgctacgaa ctcccagttc
    18721 accagcgccc agaagtgctc cagatagcca ggacgtgcat tgcgatagtc gatgtaataa
    18781 gcgtgttccc agacatcaac ggtcagcagc ggagtcgcat cggtggtcag cggagtaccc
    18841 gcgttagagg ttgaaacgat agccagtttg ccatcgctgt ttttcaccag ccaggtccag
    18901 ccagaaccaa agtttttgat cgctgcatca gtaaactgcg ctttgaaatc ggcaaagctg
    18961 ccaaaagatg cggcgatagc ttcagcgact tttccagtcg gttcgccacc ggcgttcggt
    19021 gccaggcagt tccagtagaa agtatggttc cagacctgag ctgcgttgtt gaatacgcca
    19081 ccttcagagc tgcgaataat ctcttccagt gatttacctt caaacgcggt acctttaatc
    19141 aggttgttca ggttagtgac ataagtctga tgatgcttgc cgtagtgata ctcgatagtt
    19201 tccgcagaaa tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg taattcgaat
    19261 gacattgcta ctctccttta ttattaattt gcatacgtac aatagcctta ttgtgcgtat
    19321 ggatagggta gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt gccgtaacaa
    19381 cagggtaaag ttttagcgaa gtgtttttgg ggtcattacc cgacgagcgc caacatagtg
    19441 gcgctgccag tagtcttcac tgagagaagt gatttgaatt tcctgacctg tgcgcggtga
    19501 ctgaataaat ttgccgttgc cgacatacac gccgacatga tcggctgtgc cgcgtccctg
    19561 agtacggaaa aagaccaggt cgccgttttt cagttcacta cgttcgattg gcgctgcatc
    19621 acgcaggtga tacatttcat tcgccgtacg cggaatacga attttcacca aatctttata
    19681 agcgtaataa accaggccgc tgcaatcaaa accggtacgc ggtgagctgc caccccaacg
    19741 atatggctta ccaatttgct gcatcagttt attcattgcc actttcgtag ctttttgcac
    19801 cttcgctttg tgcgcgtcgg caagagttcc tgacgcggca tttttgactt tcacacaatg
    19861 cgatttatag cccttacgtt tggtacactt ttcggtaaag ctggctgagg ccgttttatt
    19921 gacggcgtgt ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag attttttaac
    19981 ggaagaagcg gttttgcttg tggtcgtact tttactggcg gcttttttcg ctgttttctg
    20041 ggtctttttg gtggttgccg tgcttttctt tttatctgct tttttggtga tatgagtggt
    20101 agcagaactc tccctggctt gctttgaagc atgggccata ggcgttaaag gcagggtggt
    20161 aaaaagtaaa gcacagagcg tgatcgaaat acggtttatc cgcgccactg agcgtccctc
    20221 tgttaaggca ggtctatcat gcctgcgtct gatttacaaa acttgatgat tctaatgcat
    20281 agaagaagag aaagttaata gcttttattg ttaaaaaata cgtttcgttg gcgagcgcaa
    20341 aaaaatgcag cagtgaaagc taaaaatggt tataaatacg ccagtttgcc gcgcatcaga
    20401 gaagaaacga atatttgaaa tgcaacaaat agagcaaagg gataaaatag caaaagcgtg
    20461 atgaaaatgt tattttccga tgccgcactg acccgctaca atgtcagatt actgccgtaa
    20521 agaagtttga ggaagcaaga caatgagcac cactatcgaa aaaatccaac gccagattgc
    20581 tgaaaacccg atcctgctgt acatgaaagg ttcaccgaaa ctgccgagct gcggtttctc
    20641 tgcccaggca gtccaggcgc ttgccgcatg tggcgaacgt tttgcctatg ttgatattct
    20701 gcagaatccg gacattcgtg cggaactgcc gaaatatgct aactggccga ccttcccgca
    20761 actgtgggtt gacggcgagc tggtcggcgg ttgtgatatc gtgatcgaaa tgtatcagcg
    20821 tggcgaactg cagcagctga tcaaagaaac tgccgctaaa tacaagtctg aagagccgga
    20881 cgcggaataa tccgtcctgc aatactgacg gatgcgaacg ggcatccgtc agtcctttct
    20941 ctatccccaa tccagccctt gtggcgaact tgaaaatccc gcctcacgca gcagcgtaaa
    21001 catcggcgtt tgccggaccg gtagatcatt cacttctgtt agcgtaaagc gcagccgtgg
    21061 ttcgcgacgc agtgcggtag tcagcgcgtg gaaaacctct ggggcgagta attcctcttt
    21121 ttcctgccag accagcattt ttttgccacc ttgcgccaga tagagtaaca atttgccgcc
    21181 agaaacgacc accagcgcac ccgcccgacg cgttggaacc agcgttgccg gatgtgcagg
    21241 ccagggcaga agatttcccc acacattagc cggatcgttg gcagagagcg ccactggtgt
    21301 atagtggcgc gtttgcgtgg cttgtgtcgc cagatcgcgc aatcggtcaa tagtcagacg
    21361 ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga cctcgcataa ttcgcccgga
    21421 gtcttccata cttcgacaaa gcgtttgcat cgatggaaac ccgccaggga tattttcggc
    21481 tatcaccgcc tgacgactga tgatgccgta gcggtcgagc atattttccg ccagcgccag
    21541 catcctttcg gtatcgttta gtggctccac ctgcaataac gaccagcgtc cagccagatt
    21601 tggtgtgttg taagatacca gcggcgagac gggttgcgca tagacaggac gtccacgacg
    21661 agcctggtga ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg tgagggcgcg
    21721 taacggtgcc caaatgtcgc tggtgatgac gccttgccag accagcgccc ataacgcctc
    21781 ttgcagggca gagagatcaa ccgattcgcc gattttgtcg cgtatccgtt ggctgatttg
    21841 ttgtgcaaac caggctcctc cgtcagccag aacagcgact atcgcttgtt gcagcgccga
    21901 acgattcgcc tgatccgctt cggcgggagt gaacgattct gcagcatatt cctgtagatg
    21961 cagtgccacc aggccgtcat cttcacccag ttttttttgc cccgaccaga taaccgcacc
    22021 ggttgccagt aattcatcga gcatttctga cgaatagtcg cgtacgcggg caggcaggat
    22081 ctggctttcc cagagtgagg cgggtaaacc gactccggca agctgttcga tcacccgcat
    22141 cacgccatct acgccctcat aaacgcctgg cgatgttgag gcaaagagcg ccgggctacc
    22201 atcggtggcg ggtaatacgc cctgacgttc cagcagcaat cgcgcatagg tcgtggctgc
    22261 aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc aaacgcagac gacgaaatac
    22321 ttcatcgctc acccagatgt cttgttgcag attcatcacc agaccctgtt cacgcagttg
    22381 ctgaagctgc tcttcgacaa tggcaatacc gagactaaac tcatgagcca gttgttcagc
    22441 cgtgaccaga gcatgagccc ggagatagcg cagaaagaga tcgcgaagcg ggtaactgac
    22501 tctatgtaaa taaatctctg gcaatgactc tggtagtcgt acgccgaggg catcacgcag
    22561 cctggcggca tcatccatac aggcaagacg ctcctgtccg ctaatcatcg ctgggaagat
    22621 tcgttttact gcaagaagat tttccagata cgacgcaacc tcttcactgc tgcctgtatg
    22681 ccgttgcgcc aggtcttcaa cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc
    22741 ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc aactcttctt ccacctggcg
    22801 aatgacctgc gggtcgagta attcccccgg atcgacctgt ccgagtagat tgcgcagtaa
    22861 ctcgctgtcc agcgacagta cggatgcccg gcgctctgcc agcggggcgt cgctctggta
    22921 cataaattcc gcgacatagc cgaacaataa acttgtggcg aaaggcgagg gcgtagtggt
    22981 cgttacatcg gatatttgaa tttcgccacc gttcaggcga cgcatcaaac gttccagtgc
    23041 gggaagatca taaacatctt gcagacattc gcgtagggtt tcgagaatga ccggaaaatc
    23101 cggatatccc tgagcgattt ccagcaactg actggcgcgc agccgttgtt gccaaagcgg
    23161 ggtgcgatgg cccggagtgc gccccggcat taataatgcc cgcgcggcgc attcgcgaaa
    23221 acgggcggcg aaaagtgccg agctgcctac cgcctcgcgg acaatttgca gcaacttttc
    23281 tggttcaaac aaaaaaatcg cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc
    23341 aacaatgccg tcatcactgg cgaccacaga cgcgtcagcg ccccataggg catgtattcg
    23401 cccggcaatc gccagcgccc agggttcatg cacccgtctt ccatagggag agtgcaaaat
    23461 aatacgccag tcgccaattt catcacggca ccgttcgagg accagatggc gactccccgg
    23521 aacgatgccc gtcgcgttgc gttgctcgtc aatcaacccc tgaatattgg cgatcgtatt
    23581 ttcttctgcc agccacgggg gaatagtccc ggaaaagaac gcgccatccg ccagcaaatg
    23641 aagaaaatcg ccgatcatct cgcctaattc agccggacgt ccgttacctt caccacgcca
    23701 gaaggggagc cgggcagaac gacccggagc aggagtcaca atcacctgat cgcgggtgat
    23761 ttgctggatc cgccatgagg tagcgccgag agtgataatg tcgttcaccc gcgactcata
    23821 taccatctcc tcatccagtt cacccacccg ccgcgaaccg gcctgttctt caccttcggg
    23881 taataacacg ctatacatgc cacgatccgg aatggtgccg ccgctggtaa ccgccaacaa
    23941 ttgagcgcca ggtcgggcgg tcaatatccc ggtctccctg ttccagacca gtttggggcg
    24001 aaaagcagaa aaatcgccag agggatagcg cccggaaagc atatccagcg tggcgtcaaa
    24061 aacacgtctt ggcagatctt tccacggtgc ggcacggcgt acgcgggagt accattcgtc
    24121 tacctgtaat gcctccatcg ccgcggcggc aacggtttgc tgcgcaagga cgtcgagtgg
    24181 attatgcggc ggtgtcaggt tttccagcct gcctgcgaac atacactcta caatgactgc
    24241 ggaatcgact aagtcacgcc gggtacgagg gaaaaacagc cctttagata caccgccaac
    24301 ctgatgtccg gcgcgaccaa tgcgttgtaa cccactggca acagaaagcg gcgttgccac
    24361 ctgaatcacc agatccactg cgcccatatc aatccccagt tcgagactgg aggttgcgac
    24421 cacgcagcgt aactcacccg atttcagcgc ctgctcggtg attgctcgtt gttctttaga
    24481 gacggagccg tggtgtgagc gggcaataaa aacgtcgcta ctttgtacac ggttagaggt
    24541 tgcgccggag gtcgactcga aatgggccgc atcaacggcg atagacgggg aacgctgtaa
    24601 tcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt tccgccagcc cacgtgaatt
    24661 agtaaagaca atggtcgagc gatggcgcaa cacttcatca aggatacccg tttcaatata
    24721 tggccagatg gagccttccc ggccggcatg gctgtcttcg ccggtgctgc tggcgaccga
    24781 tgagacatca tccatattgg cgaccggtac gacaattcgt atctgcggat ggcgcattgc
    24841 gggcgggttg actaccgtaa ccgggcgatc gccaccaaga aatgctgcca catcgctggc
    24901 tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag
    24961 ccgctccaga cttaacgcca gatgcgcacc acgtttactg cccgctaccg cgtggacttc
    25021 atcaataatt accgtttcga cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag
    25081 atagagtgat tcgggtgtgg tgatcagaat atccggcgga ttacgggtga gtttgctgcg
    25141 ttcctgtgca ggcgtatcgc cagtacggat ccctacgcga agattgactt ccgtttcgcc
    25201 gcgccgccgc cgttcatcgg caataccctt caacgggagc tgcaagttgc gctgaacgtc
    25261 ggtgcccaga gcttttatcg gtgaaatata gaggatgcgt gaggttttac gcttatgcgc
    25321 ctcgcgggta tcttcgccgc cttcgcggaa gagccgatcg agggcgtaga gaaatgctgc
    25381 cagcgttttc ccggagccgg tcggtgcaat caccagcgca tgttcgcttc gcgccgccac
    25441 atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg
    25501 ggtcgccggt gaaaacacgt ccggcaggag cgatgaagag tctggattat ctgccattaa
    25561 cgctattccc cgtgaatatc atgcaggatg cgtggaatag cctcactata gatgcctgaa
    25621 tcattgcatg tcatcaggca tcgactcgat tacacctctt cggcggcagg tagcggccag
    25681 cctcccagac gtttccagcg gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg
    25741 tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc
    25801 tttgacaata cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg
    25861 aaagggtgga acgggttacg tttcagtgag gcgcgttctg cggcggccat cataaagctg
    25921 tgatcaaaat tggcattgtg cgccaccata atggcgcggt tacagccgct cgctttaata
    25981 cctttacgta caactttaaa aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga
    26041 tcgggatcgt tcgggtcaat gccgttgaag gcgagggctt ctggttgcaa atttgcgccg
    26101 acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc
    26161 attttcaggg tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccggct
    26221 gtttcaacat cgatcacaac aggataaaaa ccacgaaaac ggtcgcacag accggtaagt
    26281 tgagcgttat cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca
    26341 aatattactt gtcgatgcag taaagatgcg ggcgcgatga gttcacgccc ggcaggagat
    26401 tagttgccca gaccgcgacc ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc
    26461 ggatcttcca caaacgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtc
    26521 acgttacccc cgttttgacg gattttttcg cacgcttcag cggcgttatc tacgctaagc
    26581 gcgatgtgac cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt
    26641 tcaatcaccg cttcttcggt ttccgggccg tagccaacaa acgccagtga gtatttgtat
    26701 tccgggtttt cgctggtacg cagcagtttc atgcccagca ctttggtata aaaatcgatg
    26761 gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tttatcctca
    26821 aaatggctaa cagattcaaa atgttgtttt agtatagcgg cggctttacg ccgctcgcaa
    26881 tgttggatta caacgtcggg taatcggtat agccttccgc gccgccaccg tagaaacttt
    26941 cggcacgctg tgggttaagc tcagctttgc gctgcaagcg ggcgaccaga tccgggttct
    27001 caatccagtc acgaccaaat gccaccgcat caattaaccc tttgccgatc agcgtttcag
    27061 ctttttctac tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt
    27121 tttcgcggaa cgcatcagta tacggttcac cccccgccca atctggttct gacatatgca
    27181 gataagcaat gccgcgttta cccagttgtt caatcagata cagtgcatcg gcttcttcat
    27241 tcgggccgtt atccgtgttc tggaaagtac cgattggcga aatgcgaatg ccaatgcgat
    27301 cggcacccca ttcttcaatc ccggcatcga ccacttccag taccagacgt gcgcgatttt
    27361 ccacgctgcc gccgtactga tcggtacgat ggtttgaaga aggagaaagg aactgatgca
    27421 gcaaataacc gtgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag
    27481 caatggcctg acggaaatca ttgacgatac ctggaatctc ttccagttca agcgcacgcg
    27541 gcatggatgt ttcaacacgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc
    27601 ccgcgctaag tgctgaaggc gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag
    27661 aaatgcgtcc ggtgtgccac agctgcacgg ccatatgacc attttcagca tgaacgccag
    27721 cggtgatttt tttccatgcg gcaatttgct ccggactatg gatgccaggc gcacctgcat
    27781 atccttttgc ctgggcagaa atttgcgtgg cttcactaat aatcaaaccg gcactggcac
    27841 gttggcgata gtattccgcc atcaacgggg taggaatgtc acccggttca atactgcgca
    27901 gacgcgtcag cggtgccata aaaatacggt ttgccgccgt gatcgcgccc actttcagtg
    27961 gggaatacag tttttcagat gacataaagg ctcccggtcg tctggtgata aaaaatgcct
    28021 gctaaacggc aggcgtcgca ataatgtttt ttacatgggc cagcgcgttt tccagtggct
    28081 cgaaactgcg cgaaattttg gcctgcagat tcgcacccag ccatagtgcg taaagcactt
    28141 gtgcctgttg tagcggttcg ccacaaaagg ttaaacaatg gttctcacgg ccattttcca
    28201 gcgcctgaga gagcagggcg atcacgccgc gagcaccttt atccatcgcg ctgcgcatat
    28261 cttctgacag atcgcacact tcggcagaga gttttactgt caggcaacca ctgatggttc
    28321 catgttggca aaactggttc agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt
    28381 taccttcgcc ggattgcagc aactcagtca gtcgctggtg atatgcggcg taatgacgct
    28441 caagcatggc aacgccaaac gcttctttag agcgaaagta gtgatagaag gaccctttcg
    28501 gcacttcagc ggtttttagt aattcgctta gccccatccc ggtgaatcca cgttgcaggc
    28561 aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg tgtttgttca
    28621 tgcgttggag tgtagtagac cagtcggtct agtgcaagca aagttgctac tttggacatt
    28681 aacagcttca ataatggtaa taatcttatc tgaggagggc gaagtggcgg agcaattaga
    28741 gttctttccc gtccagagcc cgtgccgggg aatttgccag tctgacgaac gcgggttttg
    28801 tcgcggctgt tttcgtagtc gtgatgagcg ttttaactgg aataaaatga gcgatggcga
    28861 aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg cgcaaattac gcgcaaataa
    28921 gccagcgtca tcagacgaac cggagcaacc atcactcttt tgaacctaat cttgcgtata
    28981 ctcctctcat atctgataag aggaagcgat tatggttcag cgtattacta ttgcgccgca
    29041 aggcccggag ttttcccgtt ttgtgatggg ctactggcga ttgatggact ggaatatgtc
    29101 cgcccgccag ctggtcagtt ttattgaaga gcatctggat ctcggcgtga ccaccgtgga
    29161 ccatgctgat atttatggtg gctatcagtg cgaagcggcg tttggcgagg cactgaaact
    29221 ggcacctcac ctgcgtgaac ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc
    29281 gcgtgaagaa aacgtcattg gtcattacat cactgaccgc gatcacatca ttaagagcgc
    29341 cgaacagtcg ctaattaatc tcgcgaccga tcatctggat ctgctgttaa tccaccgacc
    29401 agacccgtta atggatgccg atgaagtggc ggacgcgttc aaacatctgc atcagagcgg
    29461 caaagtgcgt cattttggcg tatcgaactt tacgcctgcg caatttgccc tgttgcaatc
    29521 acgtctgccg tttacccttg ccactaatca ggtggaaata tccccggtgc atcagccgtt
    29581 actgctggat ggcacgctcg accaactaca acaactgcgt gttcgtccga tggcgtggtc
    29641 ctgccttggt ggtggtcgtc tgtttaatga tgattatttc cagccgctgc gtgatgaact
    29701 ggctgtggtg gcagaggagt taaacgcggg ctcgattgaa caggtggttt acgcctgggt
    29761 attacgttta ccatcgcagc cgctgccaat tatcggttca ggtaaaattg agcgcgtacg
    29821 ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa caatggtttc gtatccgtaa
    29881 agcggcactg gggtacgacg taccgtaagc acttttagga atagccgccg ttcaaaaatg
    29941 tgtcactggt ttacacttat tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt
    30001 agtctggcta ttctggcgct ggttgttgca accggcgcac aagctgccag tgaaaaagtc
    30061 gagatgaacc tcgtcacgtc gcaaggggta gggcagtcaa ttggtagcgt caccattact
    30121 gaaaccgata aaggtctgga gttttcgccc gatctgacag cattaccccc cggtgaacat
    30181 ggcttccata ttcatgccaa aggaagctgc cagccagcca ccaaagatgg caaagccagc
    30241 gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca tgaagggcca
    30301 gaaggtgccg ggcatttagg cgatctgcct gcactggtcg tcaataatga cggcaaagct
    30361 accgatgccg tcatcgcgcc tcgtctgaaa tcactggatg aaatcaaaga caaagcgctg
    30421 atggtccacg ttggcggcga taatatgtcc gatcaaccta aaccgctggg cggtggcggt
    30481 gaacgctatg cctgtggtgt aattaagtaa ttacgaggcc agcgtacctt gcggtggtgc
    30541 ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca attgccgcca gctcacgggc
    30601 ggcaggttga tgatgacggg caaggctgtt gcaaatccgc tgtaattctt caagtgtggc
    30661 cgccagtgat ttttgctgaa cgccacgctc actcatcact ccccgcaaca gtgaaataca
    30721 gttatcccgt actcgcgata acggatcgga acgcgattcc cagtcgcgca attgccagac
    30781 aacatgagaa cagttcagca gcactacacc ccagcgtaat aaccaacggc gagccagcgc
    30841 atcctggctg ttactcaact gactgacgtg atgataagtg agcgattcaa attcgctttc
    30901 actcagtgtt ggatggcggc ttagctgatc gacaaaatcc cggcgcagcg cgcgaatatg
    30961 gcggcggctt ttacgagcat ccgatcctgg acgcagaatg gcgaacgcta gccaagccag
    31021 cgcaacgcca acgatttttg ccagattatc gttaagaaaa tcagcaaaat catacaccgg
    31081 gggattagtg acagcgataa acgaacccat aaaaacaatc agttgccccc acagtgcggc
    31141 aaatttgggc atctgcgatt taagaagctg cattgtcgcc agcagtggaa agagaaacag
    31201 taaaaattgc cacagatcgc taatctggac catcaggcca aatttgacca caaagctgaa
    31261 tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac gacttaaacg gtgctgcgac
    31321 ggcggagtag agtacgcagc taattgctgc cagcgttaat gcattggcac cgacatccca
    31381 ttgcgatgca atactccatg cgccaatcat catcaacgta caaaatgtac gcagcccgct
    31441 ccacatagct tcggcgttgt cggtatgacg agccagagcg gatgtccgtg cgagtctggc
    31501 atgatcatct acaccgcttt gcagacgatg taattcctga ctactttgca gataaaggcg
    31561 gcaaaaataa cgtagtcgct gccagaaggc gacgtgccgg tagtcggcga cgttggtcgg
    31621 gcgtagcggg gcgataatac gtgcgacggt gtaagcatct gtttgcgaac tggcgagcgc
    31681 cgtcagcaat tgttcgagaa tttctcgcgt ggcacctggc ggtgagggcc agttgagcaa
    31741 catacgtcgc aggctggaga tgacactggt catacgtaat tgctggtgga gtagcgcatt
    31801 aaggcgcgcg ttttgctggc gaaaacgata gtggctccag aaagcctgga tacgcagcaa
    31861 attcatggtc agtatctgcc caatcacccc ctcatgtgct gcacgaatgg catcgtttgt
    31921 ttcaggctgc cagagtaaac tggcatgttc cagtaatcgg gcgtgcatgt ttttcaatgc
    31981 ggttaaaagg gctgtagcat cggaactgct aggcaggatc atcatcatca tgccgccgca
    32041 caaaataccg actatcacct cgcaaacgcg cgcctgagcg atatcccaca gctggctggc
    32101 ctcagtaata ttaaccatcg gaaaggcgat gatggcagcc gtgtagcccg ccagttgaaa
    32161 tgcatacgcg acgttattcg tgaagtgcgc acaggcccag gtacaaaagc caagccacgc
    32221 cgacatgctc aatagaaaaa accacggctc attgagcgta tgcccggcaa gaagcagtgc
    32281 cgcaatggct cccagtaaac tgccagcgat gcgtccgagg cttttgctga tgacaccgcc
    32341 aacggtggga aagctaacca ctgcagccga ggtcatcgcc caatagggtt catccagatt
    32401 caaataatag gcaaccgtca gcgccagaca catggcaatg gtattgcgta acgcataacg
    32461 ccattgcgcc agcgtggcct tgaaccaggg caaattgcgc aaggaccatg acgatgcgtt
    32521 cattattgac ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac atcgcctgga
    32581 actttatcca gcgcaaagcg gacgggaacg cgttgggcga gacgaaccca ggggacgtta
    32641 ggtttcacat ccggaataag gcttgagtca ctttcaacgc tttgatcata aatcgcccga
    32701 ccgatactgg aaacgtgacc ccgtaacgtt ttgttatcac tatagagggt aatttgtgct
    32761 ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa aataaccaat gacgtaaaac
    32821 gagtggctgt cgacaagggc aaacagcggt ttcccggtgt cagcgtaatc gccgatgcgg
    32881 gtagtgaggt tagtcaccca accactcacc ggggcgcgaa tttccgtttg cgccagttgc
    32941 cattgtgtct gtttcagcgt ggcctgtgcg gcattaacac tggcctgcat cgctttaaca
    33001 ttgaggttag cggtatcgag ttcttcggca gaaataaaat tttgtgagag atgacgacga
    33061 cgattcgctt cgttattggc ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt
    33121 gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg
    33181 ttaaccagtt ggttgtcttt tatattcagc tcaacaatac gtcccgacac ctgtggggtg
    33241 atagaaacct tttctgcgcg tattttcccg tcgcgtgtcc acggagattg catgtaataa
    33301 ttccacagcc accatccggc aagaaccgca actatcgcta caatgattgt tgagaaatac
    33361 ttaattgttt taattgacat agttaccacg caatcagtat tgccagagca aggcaaacgc
    33421 aaatcgcaaa cagcgataaa tccattaaca agggatgcca gatgtcaccg gcgtagatcc
    33481 agccacgaag caggcggtgt acgacaagcc agatgacgaa tccaaacgcg aaggctttga
    33541 aaaacggagg aaaatagacg gacgcaccga acaccagatc ttgtaagggc aatcctgttg
    33601 cattgagcat aaacttcaca aagtagattc ctttacgacc gaatatattg cgttagatta
    33661 ataaatattc tttaagtgcg aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt
    33721 aaccaaatgc agcaatacat ttgttttagc aatacaattg ctgcacacta ttctaaaagc
    33781 cgcataatat cttagcaagc taattataag gagatgaaat tggaatcgcc actaggttct
    33841 gatctggcac ggttggtgcg catatggcgt gctctgatag accatcgcct gaaaccgctg
    33901 gagttaacac aaacccattg ggttacgtta cacaatatcc atcagttacc tccagaccag
    33961 tcgcaaattc aactggcaaa agcgattggc atcgagcagc catcactggt ccgtactctg
    34021 gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct
    34081 aaacgtatta aactgacgga aaaggcagag ccactgatca gcgaaatgga agctgttatt
    34141 aacaaaaccc gcgcggaaat attacatggc atctccgcag aggaactgga gcaactgatt
    34201 acgctcatcg caaaacttga gcataatatc attgagttac aggccaaagg gtgaaatgaa
    34261 gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga gaaacggtca
    34321 cctgactgcc attgctggcc agtacgacac gttggcccgg agagaaacga gtgttgcctt
    34381 gtttctgtac caccatgatg gtattaccat cgtctttacg aatttccagc tcgacaccct
    34441 gcgttttgtt cattgcactc tgtacgccct gaccagctac gccacctgca acagcgcctg
    34501 ctgcagtagc cagagaacgc ccggttccgc caccaacagt attccccagg aaaccaccaa
    34561 gaacagcacc gccaattgca ccgataacgt tggaatcatc accgccctga atctgtaccg
    34621 gacgtacgtt aacgatggtg ccatagctga cattctgtac ttgtttcgct tcagaagcgg
    34681 tataaacatc ccctgacagg gtgtcgttat taacacaacc gacaagagac agacctacca
    34741 ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc tgttcaccat gaaacgccac
    34801 gcaagcatcc ctcatggtca aagtatatgg catatgtgaa tccatggtta catgttctac
    34861 caaatatgag caaatcataa tgaaagtagg atcaaccaat tataaacaaa acattcatga
    34921 tgattattga tttgaagata atgttttact accaacggta aaaatcggtc attccgcatg
    34981 gtattcacga aaagtttatg tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac
    35041 tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc agccttgatg gtgttgatgt
    35101 tgtgttggcg acaatagatg aacaccgggt cgcacagctg gcaagcttga gctggccgat
    35161 cccggtgtct ctgaaacagg ctgtactgga tatttgccag ggccagcagc ttacactttc
    35221 gcagtttgga cagcttgata ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt
    35281 gcttaaggaa caaaacctgc aggcgagaga tatagttgcg atcggctgtc acggtcaaac
    35341 cgtctggcat gaaccgacgg gcgtggcacc acacacttta cagattggcg ataacaatca
    35401 aattgtggca cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg atattgcctt
    35461 gggaggacaa ggcgcaccgc tggtacctgc gttccatcat gccttgctgg ctcactcaac
    35521 cgagcgacga atggtgctca atattggcgg catcgccaat ctgtcactgc tcattcctgg
    35581 gcagccggtt ggcggctacg ataccggtcc tggtaacatg ctgatggatg cctggatctg
    35641 gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg gcacgggcgg gtaaagttat
    35701 tctcccactg ctgcaaaata tgctcagcga cccatatttc tcgcaacctg caccgaaaag
    35761 caccggacgc gaatacttta actacggctg gctggagcgc catttacgcc attttccggg
    35821 tgttgatccc cgtgatgttc aggccacact ggcagaactc accgccgtga ccatttctga
    35881 acaagttttg ttgagcggtg gctgcgaacg attgatggta tgtggtggag gtagtcgtaa
    35941 tccgctactc atggcgcgtc tggcggcatt actgccaggc acagaagtca ccaccaccga
    36001 tgccgttggc attagtggcg atgacatgga agcattggct ttcgcctggc ttgcctggcg
    36061 gacgctggcg ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt
    36121 actgggggct attttccctg ctaacccgtg atatcagagt taactgaatt tttcttaccg
    36181 cgtctgccgt tacacttgga acgttaggga gggcgtattg ccctccagac caggaaagtc
    36241 ttcgggaaat gtctatgaaa aaactgttac tgatctgttt gccggtgctt ctcaccggct
    36301 gtagcgcgtt caatcaactg gttgagcaca tgcaaaccgc tacgctggaa taccagtgtg
    36361 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
    36421 ataatcaact actgcatctc aaacagggca tttcagcgtc tggtgcacgt tacactgacg
    36481 gaatctatgt tttctggtcg aaaggcgatg aagcgactgt ctataaacgc gaccgcatcg
    36541 tattgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
    36601 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
    36661 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgtcg cgatcttccc
    36721 gccgatccat taaccctttt tgaacgttgg ctctctcagg cgtgtgaagc caaactggca
    36781 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg ctcagcctta tcaacgcatc
    36841 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
    36901 aaagcacatc aacttgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
    36961 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
    37021 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
    37081 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
    37141 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
    37201 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgagaaaat
    37261 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
    37321 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
    37381 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
    37441 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
    37501 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
    37561 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
    37621 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
    37681 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
    37741 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
    37801 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
    37861 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
    37921 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
    37981 acaaacagta cggtgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
    38041 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
    38101 tgatcactaa agcagatggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
    38161 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
    38221 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctgg
    38281 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
    38341 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
    38401 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
    38461 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
    38521 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
    38581 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
    38641 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
    38701 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
    38761 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
    38821 gagtttccta tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
    38881 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
    38941 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt gttaagtggc
    39001 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagcc
    39061 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
    39121 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
    39181 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
    39241 gaagcggttc tggcagcgcg cgaactcatt gcgcaagggc cacaaattgt gttggttaaa
    39301 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
    39361 gaagcctggc atatcagccg tccgctggtg gattttggta tgcgccagcc ggtaggtgtt
    39421 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
    39481 gcgctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
    39541 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
    39601 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggat
    39661 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
    39721 gcataaatgc tgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
    39781 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
    39841 catccttcag tgcctcgttc acatattgca gcttcttctc cagctgcgcg cgaactgtcg
    39901 gtttgtactc ttccggtgta tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg
    39961 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
    40021 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgctacg ccttccgtca
    40081 gcaaagtacc gtcatccagc agcactgcag gcacctgccc cttagggtta acgacaaagt
    40141 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagg gtaaaatcct
    40201 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt ttgtagaaca
    40261 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
    40321 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
    40381 cagcagcttt cgccgctttg tctgcagcgt catcctgcgt catacggtgc agtttaggcg
    40441 cggtcagcag catcagtact gcaataatgg cagtagcgac accaatctgc aagaatacgc
    40501 gaccataaac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
    40561 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
    40621 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
    40681 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctt acagacacga
    40741 taccagcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
    40801 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcgcccatc ttgttataga
    40861 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
    40921 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
    40981 gtgacgttgg catctggctg tacagcacga agaagataat ggcttcgagc atcaggatga
    41041 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga
    41101 taaccacgat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
    41161 gcagccaggt ggcgatagcg atcagtgcca caacaccaat aatggtcagc agcaggttac
    41221 ggtagttgat aggctcgaag tctggttttg aaccgtactg tttaacccag cgttggcaga
    41281 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
    41341 agccgtattt cgcggccagc cacggcgtag caatcataga gaagaaagag ccgatgttga
    41401 cggacatgta gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg
    41461 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
    41521 tacccatata aacgataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
    41581 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
    41641 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
    41701 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
    41761 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
    41821 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
    41881 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
    41941 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
    42001 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
    42061 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcac
    42121 tgagaggggg gtgatggctt aacggcgata tgttctgcta tttagcgttt ttactagtga
    42181 gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata tttgttgcgt
    42241 gtgtaaattt aaatgatttt aacctgattc ttgcaatgtt tttacattgc gacagcatcg
    42301 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
    42361 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attcacgtta
    42421 aaaagaagtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
    42481 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
    42541 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
    42601 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
    42661 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
    42721 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
    42781 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgctc cagcaagata
    42841 cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt tttgatatag
    42901 gttttcaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc caccgggtag
    42961 agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag cagtacggca
    43021 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
    43081 cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc cctgtttcac
    43141 cgtttggcaa tgcacgttcg gcagctgctt cagtacggcg ctttttcatt ctttcatcaa
    43201 tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctgga ggcagcatcg
    43261 ccagcaggaa aggggagtcg gtgtggaaaa tttccacgcg taatactttt gcccagctac
    43321 ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg cgtagtgaac
    43381 ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc agtgccgaaa
    43441 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
    43501 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
    43561 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
    43621 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
    43681 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc aacagaggac
    43741 aaaggccgag caactggacc agcgcagagt tgtttttcca caacccctga acaataacgt
    43801 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
    43861 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
    43921 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
    43981 ctgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg cgcagttcga
    44041 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
    44101 cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
    44161 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
    44221 ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgca ttgttatagc
    44281 gttcggctgg cagcacctga tcaaataatg ccttttgttg cagactggcc tgttcagcaa
    44341 tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc gctgcaaata
    44401 gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
    44461 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
    44521 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
    44581 ccgcaagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta accgggtcag
    44641 tcaaaataaa gaatgcgccg agcatggtcg caccagacag cagatgaatt tgcggtgctg
    44701 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
    44761 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacagg ccgccagcca
    44821 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
    44881 attgcataat ctgttcaacc gaatgaccgg cacggacaga ggttttaaat gtatccagcg
    44941 gtgtcgcctg actaatgcca tcaataccta agcgtagtgt gttcatatca ccaccactgg
    45001 cggtatgccc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
    45061 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
    45121 aaccaatcat tgctggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
    45181 tgatcaccgc aaacaccgta cccagcacga ccatccacca tggcgcgagg gggggaatac
    45241 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
    45301 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactaacc gatgccaaca
    45361 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
    45421 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
    45481 agctagctat tctgaatacc atttagtcct cgtttacaac cttctgctgg gcggcttttt
    45541 tggcctgagc gcgggcaata gccgcggcaa ctgccgcttt gcgcggatca acctgttctt
    45601 ctggttctgc gttagtctgt tgctgttcca gcttgcgcgc tttggcacgg gcaatagcgg
    45661 cttcgacggc ggctttgcgc ggatcaacct gttcttctgg ttccgcatta gcctgttgct
    45721 gttccagttt gcgcgctttg gctcgggcaa tagcggcttc gacggcggct ttgcgcggat
    45781 caacctgttc ttctggttcc gcattagact gttgctgttc cagcttgcgc gctttggcac
    45841 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
    45901 tagcctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
    45961 ctttgcgggg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
    46021 gcgctttggc gcgggcgata gctgcttcaa cggcagtttt acgtggatca gcaacggttg
    46081 ctgcgtcgtt agtttgctgc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
    46141 cccgtgctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
    46201 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
    46261 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagcc gctttttcgc
    46321 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    46381 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aggggaatat
    46441 tgctcgggca aacccacgcg caagccccac attcaatgca atcagcaatg ttatgcgtgg
    46501 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatccg
    46561 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    46621 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    46681 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    46741 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    46801 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    46861 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    46921 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    46981 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    47041 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    47101 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    47161 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagcc gcgttgataa
    47221 tcaacgtttc aatcttatct ccgccaccct gcaatttaac gcctgtcggg aatcctgcac
    47281 cgcccagccc ggctacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    47341 tgcgataatc ggcccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    47401 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    47461 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    47521 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    47581 gtttcagtgg aataacaaaa cgctgcgcca gtggtacctg gcgcaggggt gtaccgttgg
    47641 actgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    47701 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    47761 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    47821 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    47881 atggcacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    47941 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    48001 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    48061 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    48121 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    48181 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    48241 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    48301 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    48361 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    48421 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    48481 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    48541 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    48601 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    48661 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    48721 acgccagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    48781 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    48841 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    48901 cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga
    48961 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    49021 gccaacaaag taaacagcaa ctggcgaacg gctatgccat cttccacggg tgaaaaagcg
    49081 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    49141 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    49201 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    49261 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    49321 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    49381 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    49441 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    49501 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    49561 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    49621 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    49681 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    49741 gtgatgat
//
LOCUS       DAZFWP020000013        47739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFWP020000013 DAZFWP020000000
VERSION     DAZFWP020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.013"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            97..729
                     /gene="comR"
                     /locus_tag="ACHK0Z_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     CDS             97..729
                     /gene="comR"
                     /locus_tag="ACHK0Z_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU4500782.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(791..1330)
                     /locus_tag="ACHK0Z_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     CDS             complement(791..1330)
                     /locus_tag="ACHK0Z_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4500783.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1540..2844)
                     /gene="ndh"
                     /locus_tag="ACHK0Z_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     CDS             complement(1540..2844)
                     /gene="ndh"
                     /locus_tag="ACHK0Z_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU4500784.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADKGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3244..3786)
                     /gene="ycfP"
                     /locus_tag="ACHK0Z_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     CDS             complement(3244..3786)
                     /gene="ycfP"
                     /locus_tag="ACHK0Z_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU4500785.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPNENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3809..4834)
                     /gene="nagZ"
                     /locus_tag="ACHK0Z_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     CDS             complement(3809..4834)
                     /gene="nagZ"
                     /locus_tag="ACHK0Z_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU4500786.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFKRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4845..5669)
                     /gene="thiK"
                     /locus_tag="ACHK0Z_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     CDS             complement(4845..5669)
                     /gene="thiK"
                     /locus_tag="ACHK0Z_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU4500787.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFHSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5650..6291)
                     /gene="lpoB"
                     /locus_tag="ACHK0Z_RS03610"
                     /old_locus_tag="pgaptmp_000722"
     CDS             complement(5650..6291)
                     /gene="lpoB"
                     /locus_tag="ACHK0Z_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU4500788.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6305..6682)
                     /locus_tag="ACHK0Z_RS03615"
                     /old_locus_tag="pgaptmp_000723"
     CDS             complement(6305..6682)
                     /locus_tag="ACHK0Z_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU4500789.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6685..7044)
                     /gene="hinT"
                     /locus_tag="ACHK0Z_RS03620"
                     /old_locus_tag="pgaptmp_000724"
     CDS             complement(6685..7044)
                     /gene="hinT"
                     /locus_tag="ACHK0Z_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU4500790.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7378..9567
                     /gene="fhuE"
                     /locus_tag="ACHK0Z_RS03625"
                     /old_locus_tag="pgaptmp_000725"
     CDS             7378..9567
                     /gene="fhuE"
                     /locus_tag="ACHK0Z_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU4500791.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9627..11060)
                     /gene="ptsG"
                     /locus_tag="ACHK0Z_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             complement(9627..11060)
                     /gene="ptsG"
                     /locus_tag="ACHK0Z_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU4500792.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11355..12152)
                     /locus_tag="ACHK0Z_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             complement(11355..12152)
                     /locus_tag="ACHK0Z_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4500793.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12163..13167)
                     /gene="holB"
                     /locus_tag="ACHK0Z_RS03640"
                     /old_locus_tag="pgaptmp_000728"
     CDS             complement(12163..13167)
                     /gene="holB"
                     /locus_tag="ACHK0Z_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU4500794.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13164..13805)
                     /gene="tmk"
                     /locus_tag="ACHK0Z_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             complement(13164..13805)
                     /gene="tmk"
                     /locus_tag="ACHK0Z_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4500795.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13795..14817)
                     /gene="yceG"
                     /locus_tag="ACHK0Z_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(13795..14817)
                     /gene="yceG"
                     /locus_tag="ACHK0Z_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU4500796.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14820..15629)
                     /gene="pabC"
                     /locus_tag="ACHK0Z_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             complement(14820..15629)
                     /gene="pabC"
                     /locus_tag="ACHK0Z_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU4500797.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15749..16990)
                     /gene="fabF"
                     /locus_tag="ACHK0Z_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             complement(15749..16990)
                     /gene="fabF"
                     /locus_tag="ACHK0Z_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU4500798.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17078..17314)
                     /gene="acpP"
                     /locus_tag="ACHK0Z_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(17078..17314)
                     /gene="acpP"
                     /locus_tag="ACHK0Z_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4500799.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17525..18259)
                     /gene="fabG"
                     /locus_tag="ACHK0Z_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     CDS             complement(17525..18259)
                     /gene="fabG"
                     /locus_tag="ACHK0Z_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU4500800.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18272..19201)
                     /gene="fabD"
                     /locus_tag="ACHK0Z_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             complement(18272..19201)
                     /gene="fabD"
                     /locus_tag="ACHK0Z_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4500801.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19217..20170)
                     /gene="fabH"
                     /locus_tag="ACHK0Z_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             complement(19217..20170)
                     /gene="fabH"
                     /locus_tag="ACHK0Z_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU4500802.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20238..21308)
                     /gene="plsX"
                     /locus_tag="ACHK0Z_RS03685"
                     /old_locus_tag="pgaptmp_000737"
     CDS             complement(20238..21308)
                     /gene="plsX"
                     /locus_tag="ACHK0Z_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU4500803.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21389..21562)
                     /gene="rpmF"
                     /locus_tag="ACHK0Z_RS03690"
                     /old_locus_tag="pgaptmp_000738"
     CDS             complement(21389..21562)
                     /gene="rpmF"
                     /locus_tag="ACHK0Z_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU4500804.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21614..22135)
                     /gene="yceD"
                     /locus_tag="ACHK0Z_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             complement(21614..22135)
                     /gene="yceD"
                     /locus_tag="ACHK0Z_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU4500805.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22334..22918
                     /gene="yceF"
                     /locus_tag="ACHK0Z_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             22334..22918
                     /gene="yceF"
                     /locus_tag="ACHK0Z_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU4500806.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23030..23989)
                     /gene="rluC"
                     /locus_tag="ACHK0Z_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             complement(23030..23989)
                     /gene="rluC"
                     /locus_tag="ACHK0Z_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU4500807.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24562..27747
                     /gene="rne"
                     /locus_tag="ACHK0Z_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             24562..27747
                     /gene="rne"
                     /locus_tag="ACHK0Z_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU4500808.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVVVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27943..28896)
                     /gene="flgL"
                     /locus_tag="ACHK0Z_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             complement(27943..28896)
                     /gene="flgL"
                     /locus_tag="ACHK0Z_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4500809.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28908..30551)
                     /gene="flgK"
                     /locus_tag="ACHK0Z_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             complement(28908..30551)
                     /gene="flgK"
                     /locus_tag="ACHK0Z_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4500810.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30617..31558)
                     /gene="flgJ"
                     /locus_tag="ACHK0Z_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             complement(30617..31558)
                     /gene="flgJ"
                     /locus_tag="ACHK0Z_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU4500811.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKVGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31558..32655)
                     /gene="flgI"
                     /locus_tag="ACHK0Z_RS03730"
                     /old_locus_tag="pgaptmp_000746"
     CDS             complement(31558..32655)
                     /gene="flgI"
                     /locus_tag="ACHK0Z_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4500812.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32667..33365)
                     /gene="flgH"
                     /locus_tag="ACHK0Z_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             complement(32667..33365)
                     /gene="flgH"
                     /locus_tag="ACHK0Z_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4500813.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33418..34200)
                     /gene="flgG"
                     /locus_tag="ACHK0Z_RS03740"
                     /old_locus_tag="pgaptmp_000748"
     CDS             complement(33418..34200)
                     /gene="flgG"
                     /locus_tag="ACHK0Z_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4500814.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHK0Z_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHK0Z_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU4500815.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHK0Z_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHK0Z_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4500816.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHK0Z_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHK0Z_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU4500817.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     HGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHK0Z_RS03760"
                     /old_locus_tag="pgaptmp_000752"
     CDS             complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHK0Z_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4500818.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVGDVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHK0Z_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHK0Z_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU4500819.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38029..38688
                     /gene="flgA"
                     /locus_tag="ACHK0Z_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             38029..38688
                     /gene="flgA"
                     /locus_tag="ACHK0Z_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4500820.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38764..39057
                     /gene="flgM"
                     /locus_tag="ACHK0Z_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             38764..39057
                     /gene="flgM"
                     /locus_tag="ACHK0Z_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4500821.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39062..39478
                     /gene="flgN"
                     /locus_tag="ACHK0Z_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             39062..39478
                     /gene="flgN"
                     /locus_tag="ACHK0Z_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU4500822.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTIHRGGKKISI"
     gene            complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHK0Z_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHK0Z_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU4500823.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPALLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41163..42086)
                     /locus_tag="ACHK0Z_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             complement(41163..42086)
                     /locus_tag="ACHK0Z_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4500824.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42088..42735)
                     /locus_tag="ACHK0Z_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             complement(42088..42735)
                     /locus_tag="ACHK0Z_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU4500825.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSCAARMYEFSDMAEVESTLEQLANREDGPFVVRLARELGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHK0Z_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     CDS             complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHK0Z_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4500826.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHK0Z_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     CDS             43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHK0Z_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU4500827.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44838..45485
                     /gene="grxB"
                     /locus_tag="ACHK0Z_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             44838..45485
                     /gene="grxB"
                     /locus_tag="ACHK0Z_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU4500828.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45619..46179
                     /locus_tag="ACHK0Z_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             45619..46179
                     /locus_tag="ACHK0Z_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4500829.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHK0Z_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHK0Z_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU4500830.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47405..47650
                     /gene="dinI"
                     /locus_tag="ACHK0Z_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             47405..47650
                     /gene="dinI"
                     /locus_tag="ACHK0Z_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU4500831.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11553 a        12906 c        11872 g        11408 t
ORIGIN      
        1 tagaaagcaa ttatttttat gggatctgga ctggtgaacg gaggtgatta tttttataat
       61 aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc aacacaatgt
      121 gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc
      181 atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg
      241 accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg gttatttcgt
      301 gccgttctcg accgctatat cgatcgtttt gccgctaagc atgaagcaca gctgttttgt
      361 gaagagaaaa gcgtggagtc tgcgctggct gactattttg ctgccatcgc caactgcttt
      421 accagcaaag acaccccggc aggctgcttc atgatcaaca actgcaccac cctctcccca
      481 gattcaggag atatcgccaa tacgttgaaa tcacgccatg cgatgcaaga gcgcactttg
      541 cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca
      601 catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc acgcgaaggt
      661 gcatcgctgg aaaaactaat gcagattgcc ggaacgactt tgcgtttatg gcccgaactg
      721 gtgaaataag tcgcaaaaaa agcccctcag cctgagcgaa tgaggggcca aattgcagag
      781 tacagccttg ttatactttg ttattcaaaa tcagttgccc attactatct agcgggatct
      841 gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc ttataggtca
      901 catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc tgttgcgtag
      961 tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttaccggca tatccaccac
     1021 ccagcgcccc cacaacagtg gcgacatctt taccgcgacc accgccaaac tgatgcccta
     1081 tcacgccgcc agcaacagcg ccgagcaccg acccggtaat gcgattttca tcctgcaccg
     1141 gtcgacgatg ggtcactgtg acgttgcgac actcctgacg cggtgtttta accgtttcct
     1201 tgattggggt tgcagaaaca acctgagcgt attgcgggcc acgttcaaac acgttcagac
     1261 tggccactgc cgctacgccc agcgcagctg cgacaccaat cccgataccc gccaacattg
     1321 atttattcac gggaacacct ccttctttgc agttatgcca atcatgcaag cgaaggagaa
     1381 aagcagctat cagacagaag atgaaaagcg cgggaatggc tggaaatact taaggattcg
     1441 gaggagtctg aaggaatgcc tgatgcgacg cggctgccga tcgcgctgtt cctcaagggc
     1501 ggataaggcg tttatgccac atccgccagt gtacgtcgat taatgcaact tcaaacgcgg
     1561 acggataacg cggttaatac tccccaccag catcattaat ccggttttaa agtaaccatg
     1621 cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa ttcgtccttc
     1681 aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccaacggtgg agaagttcga
     1741 cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct taccattcat
     1801 ctgcgccaga atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct gagcacgcgg
     1861 cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc
     1921 tggatcgcgg gtggtttgca gcgtcggttc caccaccagc tggttgatac ggttagtttc
     1981 aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc acaccatcag
     2041 atcagcctca atatattcgc catctttagt gtgcaggccg cctttatcag cactggtgac
     2101 catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga
     2161 gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg ttacgttcag
     2221 ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgaccg cgttgtgcaa
     2281 ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc
     2341 atttgcgccc aggttggcgg agtatttcag gaagagattc agcatctcct ggtgaaagcg
     2401 acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacacctg gcgtattgaa
     2461 atcgttagag gtgctaccca gcgccattac cagggtgtca taggcgattt tacgttccgg
     2521 aactagcagt tcacctttct catcgcgcag ttctgcaata gtgattgttt tcgcttcacg
     2581 atcaatatca atgacggaac ccagctggaa ctggaaacca tgattgcgcg catgggccag
     2641 atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt gcagcagcgg
     2701 tttccacaga tggctgtggt tacgatcgac cagcgtaatt ttggcttttt tcttgcgtcc
     2761 cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc cgacaatcac
     2821 aatctttttc aatggcgtag tcaacgtgac ccccttaaat ttattaacca attgttaatt
     2881 aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc
     2941 tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa tcaagttttg
     3001 ttgctgaaaa gataggcgac tttaccgggt gagatcaaaa aacagagttt attgtttatt
     3061 atttgagcaa gcccggaagg gcgatatagg gtgaagcgta tatcgagggc gtttggtcgt
     3121 cctgataaga ggcgcatcag gcattgatgc tcgaatgtcg gatgcggcgt gaacgcctta
     3181 tccgacccac agaatccgga gcgcatcagg taccggcaag gttacctgat gcgacgaggg
     3241 catttacccg agggttttga acgctttaat gcgctgtaaa tgcggggaga tattcttgaa
     3301 tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa tgatgcagct cttcagatgt
     3361 ccgctggctg ttaagcgctt catcattacg cgacaaaatc accaggcaac gatcgcgatt
     3421 cttctcacgg aagttggtca cacacttagt ggcaatatcg gcatactctt ccgggcgatc
     3481 aatcttgcct tccatgttct cattagggaa caaattaggg ttgaagatca cctggcggat
     3541 atcgcaaaga aaaccaatcc gttccgccca gtatccgccc agaccaacgc cgcaaatcag
     3601 cggacgctcg tcgacgttca gttgcaacat tttgtccact tctttgagca gatgctgcat
     3661 atcatgtttc ggatgccgcg tactgtagct tatcaagcgt acatccgggt caataaactg
     3721 caattgtaag actttctcgt ggttacccgg actgttagag tcaaaaccgt gtaaatagat
     3781 aatcatcgct tcctcacata agccagggtt agtgacctgc tttctcttcc tgccagcgtt
     3841 catgtaactg attcagacgg gtgctgatcg ctttccagcg agccgagtcc atcagttcct
     3901 gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg ttctgccttg atcggtgaca
     3961 gattatctaa cacgctgacg gcccctttac gattattgca gaccaggatc atatcgcaac
     4021 ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc cataatcgcg gcaccttcca
     4081 tcgataaatc gtcagagaaa atcacgccgt caaaacccag ttcctgacgc aaaacggttt
     4141 tcagccagta gggagaaccg ctcgccggac gcggatcaac atcactgtag atcacatgcg
     4201 caggcataat ggcgtcgagt ttattttcgc ggattaagga actgaagacc gacatatctt
     4261 tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt ttctttgtgt gagtctgccg
     4321 ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt cattccggct tcatgcatac
     4381 catcaataaa ccggctggca attgccaggg ctttttgtgg atcggcatga taagaacgct
     4441 cgccaatcgc cgcgctgata tgcccgacat ccagcacagg cgcaaagctg atatcaatat
     4501 ccatagcgat catttcgctg gccatcagcc aacccgcctc ttgcgccagt ttgccgccct
     4561 cttccattcc caacagcgca gcaaaggatt gtgctgccgg taagcgggta aaaccttcgc
     4621 gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac caccagatga ttgcgcgaag
     4681 ctgcgcggat ctggcgcacc agttcacgca actgggcagg atcatgatag ttacgcttaa
     4741 agagaatcag ccctcccacc agcggatgcg caagtatttc acgctcttcc gcgtccagtt
     4801 cgtaaccttc gacatccaac attactggac ccacactgct ctccttattg ttttattaat
     4861 agctgccgcc aggtgtcatc ggccagcctg ataaattgtt gatcgccggt ttgtcgccag
     4921 cggtactcaa accaccctgc tttgagcatc agcagccagg gaaaccatcg cctgacctga
     4981 cgccataatt gcgccggata aatcttcgcg cgagtggcat agtcattgac caattgccgg
     5041 tgctggtcag tattttccac ccacaccgcc gccagttcca gcgcgatatc accatctccg
     5101 gcatactccc agtcgatgag ttttaaccct gacgcgctat gcactaaatt tccggcgtgg
     5161 acatccatat gcaatggact taagcgtaaa ggccgtggtt cccgcgcttt gcgcagacgt
     5221 tttaaccttc gcagccaacc cactgtccgc cgcgccggat cgctttgctg ccagtacagt
     5281 tccagtaacg gcaacagcgt tattcgccag ccaaaacgcg gttgttgatg tagataatac
     5341 agcaagcctg ccagttcgtt ggtatccggc aaatacgttt ttacctcgcc gggcagatag
     5401 tcgaccacca tccagtcacg gagatataaa tgcggcttcg gtgcaataca tgcgggtaat
     5461 tgtgataaag cccgatactg gcgcaagaac gcgaaccgcg gcgcatcagg atcgtgcggc
     5521 tgacgtacaa caaaacgctg gccctggtgt tcaatgagaa aactcccgcc actaagccca
     5581 ctatgaaacg tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc
     5641 atgatgggat tattgctgcg aaacggcacc tttacctgac cagataattt cgcccgtctg
     5701 caccagcatc agctgcattt gtagggtcgg agcgttaacg ttgccagagg cgctggagta
     5761 cagcacgtaa tgagcgccga cattgcgggc aatgcctatg gctttactac gagtacctaa
     5821 actgtcctgc ggcgacaaac ctaactgttg cttagccatc gacagctgct gggcggaaac
     5881 cagggtaaat ttcccgttat tagccagcgc atttcgcagc gtttcggtcg cttctgcggc
     5941 attcagcgaa ccgttagtac ggttgttaac gctatcaacc agcaggacgc tacccgcagt
     6001 caccccgtca gccccaagca tcttactgac catcggctgc attgcgccat tccagtcata
     6061 atggcgaata tgcggcgcag gcggtgccgt ttgatcttcg tgctcaattg ggcctggctg
     6121 ctgcgggatc gtcggcaccg agggcactgt ggggacaggc tgttgtggct cggctggttg
     6181 ttccggcgct ggtttcactt cttctaccgg tgcaggttca cgttgcccca cacacccggc
     6241 gagaaacatc gccagcgcgg taatcaaggc gtagcgactc atttttgtca tcaagattca
     6301 ccccttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt
     6361 acagcgttac cgccgaatgt gcgggaatgc taacgctgcg tggcctttcc agaggatgca
     6421 tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc
     6481 tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct
     6541 tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat
     6601 tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacgcc agagacacca
     6661 gcccaaagca tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg
     6721 acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt
     6781 attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc
     6841 tacggtgatc atgcgtccca gcgcctgctc atgctcagct gagacgtcgt tcacagtcgg
     6901 aatgaggata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa
     6961 cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt
     7021 gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc
     7081 atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa
     7141 ccttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt
     7201 cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac acgacaaata
     7261 tgaatgcgta tatttctcat ttgcatttac aaacaaaatt attcgcacac taaatataat
     7321 cgctcatcac cctcctccgg catgagcctg acgacaacat aaaccaaggg atttcaaatg
     7381 ctttcaacac aatttaacag ggataatcaa tatcaagcca tcaccaaacc gtcactactt
     7441 gccggttgca tagcactggc actattacct tctgccgctt ttgctgcacc agccactgaa
     7501 gaaacggtga ttgttgaggg ttcagccaca gctccagatg atggcgaaaa tgattacagc
     7561 gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat tcctcagtcg
     7621 gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct gggcgaagtg
     7681 atggaaaaca cgctggggat cagcaaaagt caggcggatt ccgatcgtgc tctttattat
     7741 tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta ttttgaatcg
     7801 cgctggaatc tgggcgacgc actttctgat atggcactct ttgaacgcgt agaagtagtg
     7861 cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat taatatggtt
     7921 cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata cggtagctgg
     7981 aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg taaaatccgc
     8041 gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta caacagtgaa
     8101 aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac gctttcagcc
     8161 ggttacgaat atcagcgcat tgatgttaat agcccaacct ggggcggttt accgcgctgg
     8221 aatactgatg gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc tgactgggcg
     8281 tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt tgctgatacc
     8341 tggcaagcga cgctgaatgc cacccactct gaagtcgaat ttgacagcaa aatgatgtat
     8401 gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag taattatgga
     8461 cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa agttgatgcg
     8521 ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa tctaatgttt
     8581 ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa catcttcccg
     8641 gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga ctggtcacca
     8701 cagagcctgg cgcaggacga taccacacat atgaaatcgt tatatgctgc cactcgtgtc
     8761 acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg gcgggttgat
     8821 acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct ggtgtttgac
     8881 atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc gcaaaatgat
     8941 cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga gctgggtctg
     9001 aaatcggact ggatgaatag ccgtctgacc accacgttag ccatcttccg tattgagcag
     9061 gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga aaccgcctat
     9121 aaagcagtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg cgcaattacc
     9181 gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa cgaaggaaac
     9241 gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta tcggttgcct
     9301 gtcatgccag agttgacagt cggcggtggt gttaactggc aaaatcgcgt gtataccgac
     9361 accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct ggtggatctc
     9421 ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa taacctgttc
     9481 gacaaaacct acgataccaa cgtggaaggt tctatcgtct acggcgcacc gcgtaatttc
     9541 agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc catctggctg
     9601 ccttagtctc cccaacgtct tacagattag tggttacgga tgtactcatc catctcggtt
     9661 ttcaggttgt cggatttagt accgaaaatc gcctgaacac cagaaccagc aaccactacg
     9721 cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc agcaacgctg
     9781 acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc accaaatgca
     9841 gcaaccagag ccggtgccat ttcgctggta cctgtcgctt ttgcatcttc agtcgcgtct
     9901 tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat ggtgtagtaa
     9961 acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct gttaccagac
    10021 agaacgatga agtcgatcag accgtgcgag aacgacgtac cgtcacgcat ccccagaaga
    10081 atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag gatcggcgca
    10141 acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga ggtcagcgcc
    10201 gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga gtgccagata
    10261 gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga cagtttaccc
    10321 gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg acctgctgcg
    10381 ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg cagaccaaac
    10441 ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg gttctggtaa
    10501 gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat gaaggacagc
    10561 acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg tttaccggca
    10621 aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat gtacgctgcg
    10681 atcgcaccgg agataatccc tccgagtacg ccagtatccg ccaggtgttt agaggcgatt
    10741 tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt aaccatgatg
    10801 ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa gccgagggcg
    10861 acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc ttctgccata
    10921 acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc cagcagaata
    10981 cctgcgatag gcagtacgga taccggcagc atcagcgatt taccgacctt ttgcaggtta
    11041 gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt tttttacgtt
    11101 ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc gccctttatt
    11161 tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac gtttcaactt
    11221 cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt tcaaacgtgg
    11281 ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta attacgagct
    11341 ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg aaacagacgg
    11401 gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt caacacagcc
    11461 atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg atgcggtacc
    11521 ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac ataacgcgca
    11581 gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga gatgtaaaat
    11641 ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg tagtacgcca
    11701 ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg ggcgtcacgg
    11761 gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg gatgaaggac
    11821 tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt ttcacccagc
    11881 gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta gggatcattc
    11941 tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc taccagatcc
    12001 cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt cacatcgcgt
    12061 gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata atccagacca
    12121 tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat gaggaaccgg
    12181 tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg tgatgagaag
    12241 ctcgcggttg atgcctgtaa cagacattaa ctgttcacga atgtggcaaa catcccccag
    12301 tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca ggcccggcac
    12361 atcaacattg gtcacctgcg cagcaccatg atggcgtttt agcgcatcca tcagcaacgt
    12421 tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcta gcagcgaata
    12481 ccagtcgccc gatggcacgc tatatgccaa cgcctgacac aatgtttcac gagcctgcca
    12541 gttatctccc tgaaacaacg ccagtgccgc gccaggcgaa ccggcgctta agcgcaatgc
    12601 ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca cggcgtactg
    12661 ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg ccagtaaacg
    12721 ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt caagcgtttt
    12781 cagcaatgcg ttagccgcgg cgtcggttag taaggcagca tcggttaccc agacaacttt
    12841 cgcaccacct aagcgtgcgt gctcattcag cttttcggtg acctcacgta ccgcatcaat
    12901 gcccaacgca tttttccctt tttcgggagc cagggtgtag taatcgggat gcgtgccagc
    12961 ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg gttgttggca
    13021 gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg gtaacgcctg
    13081 aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt tttcgaaatc
    13141 aggtcgtaac catggatacc atctcatgcg tccaactcct taacccagtg ggtcacggta
    13201 gtgcggattg catccatcac ggcctccagc ggctgggtgg catcaatggt atgaatactt
    13261 ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa atcgaaagac
    13321 tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc aacttccggg
    13381 gtaacatcga gatagagcgt taagtcgggg cgaaaatccc cgagaacagc atcacgcagt
    13441 gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg agtggagaga
    13501 tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat gaccgtttct
    13561 accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt aatgacttca
    13621 tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag ttgcgtaccg
    13681 ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt ctcaaccacc
    13741 acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat atacttactg
    13801 cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat ggctggcaag
    13861 attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat acggcgtttt
    13921 tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg cacccggcgg
    13981 caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag
    14041 tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct gcaggcgcat
    14101 accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac tggcaacggc
    14161 ggtttctttt tcgataattg atgccatcgt caccagctga tttttatctt tataaggcag
    14221 accgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct tgtgcgctcg
    14281 cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg gccagaacca
    14341 accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg cgtacttatc
    14401 gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt agtcgctcag
    14461 acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt ccaacaattt
    14521 cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga
    14581 aagatccggt tcgatacgca gcagccattg aaaaacccgt gggcgattga tgatcttatc
    14641 agcataaagc tgttcaccga gcgccagacg tccggtccct ggcttcaggg taaatatcgt
    14701 ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga cgcccacacc
    14761 agcggcgata cccagtacca ccagcaataa caagattatc aataacactt ttttcatgac
    14821 taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag
    14881 acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat aaccatctca
    14941 tctgcctgca acgcctcttc cagagaagct tgcacttcga caagctgata agaggattgt
    15001 gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg atccagtcgc
    15061 ggcgtataaa ctacgttgcc cttccgccag aacaaattag ccgcacagca ttccgtaacc
    15121 cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag atgagagcga
    15181 atcaatactt gctcaagacg attgagatgt ttaatacctg caagatgagg attgcgcccc
    15241 agccgcaccg ggcttagcgc caacgtcatc ccctcgttac gcaaacggtc gtaatgtgca
    15301 ggataagccg taacggagag aatccgcgtt gccggtccac tgttcaatgt gctgtaccct
    15361 cgcccgccac taccgcgact gatcacgact ttcagtacac cattttgctg ttctgctgcc
    15421 agcgttttca tctcttgttc aagctgtggc cagaagtcac aggaaatcat caaccgctga
    15481 caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc gtcgataact
    15541 ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac tgccagcgat
    15601 tcctgcttat aaccgttaat taagaacata ccggctcctt atcagagaat tgttagtgtg
    15661 gcagcatgtt cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg
    15721 accttttata agggtggaaa atgacaactt agatcttttt aaagatcaaa gaaccattag
    15781 tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc tgacgtgctt
    15841 catgcggtac gaaatccaga tcgcaacctt catccgggtt atccaggttg atggttggcg
    15901 gaacagcctg atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac
    15961 ctaacagatg accggtcata gatttcgtgg agcttaccaa cacacggctt gcagcttcac
    16021 cgaagatggt tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt
    16081 gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca
    16141 ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc
    16201 tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt
    16261 actcttcaag taccagcata ccggcaccat cgcccagtac gaagccatca cgctctttat
    16321 cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc
    16381 caaaaccacc aacacccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt
    16441 cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg ccggaagtac
    16501 aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg
    16561 ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt
    16621 tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg
    16681 cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct
    16741 gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg
    16801 agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc
    16861 tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg
    16921 tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac
    16981 gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaaagataa
    17041 aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg ccgttgatgt
    17101 aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga atctcagtat
    17161 caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc gcgcccaggt
    17221 cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc tgttcgccga
    17281 taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta tcaaaactcg
    17341 ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca
    17401 ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc aaatcatttt
    17461 gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc
    17521 gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg atgtaagctg
    17581 cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac
    17641 ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca cgtgtcatgt
    17701 ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc
    17761 gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac
    17821 cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca tgacgctttt
    17881 tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg ttggtttcga
    17941 taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga gtgataccgg
    18001 cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc agaacagatt
    18061 cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttagca cctaaataat
    18121 cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc gcgccacggg
    18181 ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt accagtgcga
    18241 tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct gccatcgctg
    18301 aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtta
    18361 gcactttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc atgtactcaa
    18421 cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca cgaatggcat
    18481 caccattggt ttcgcatttc acatcaacgt tattcacaac aggaactgtt ggtgcgttaa
    18541 aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc agcgcacagt
    18601 gagacggtac gctcactggt aacggcagcg cgcgttttgc gcccgcagct ttacaggcag
    18661 cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc ggagagttaa
    18721 agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc gcaatagacg
    18781 catcatccag accgatgatt gccgccatag cgccagtgcc ttccggtacg gcttcttgca
    18841 tgaacttacc gcgcatctca accagacgca ccgcatcagc gaaatcaatc acgccagcgc
    18901 aaaccagcgc ggagtattcc cccaggctgt gaccggccat cattgccggt gctttaccgc
    18961 cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct ggctgggttt
    19021 gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac aggtcgtagc
    19081 ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc gccgccatat
    19141 cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca aattgcgtca
    19201 tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat ccaccgccaa
    19261 aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca gcttcatcca
    19321 gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc accacgacat
    19381 tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg ttagcctgat
    19441 gcggaaccag ccagtccagt tgagaacggt caagattatt tgccgccagc gtctcatcaa
    19501 cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc atcgtcagat
    19561 gaattgaatt ctctggattc acacggtcag cattaggcag cgtcagcaac tcaccatagc
    19621 taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca gccagcaccg
    19681 cagcgcccgc gccatcgcca aaaataataa tagtcccacg atcggttgga tcgcaggtgc
    19741 gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat ttcacgtatt
    19801 gatcggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg tcaaatgccg
    19861 ggcaaccttt aatgcccagc atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg
    19921 tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg cccgccatct
    19981 caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt ggcgcggcaa
    20041 tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg tccaccattt
    20101 tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta ccaataatct
    20161 tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca aactgcgagt
    20221 tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa
    20281 ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg tcgctgcacc
    20341 gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc accatgactt
    20401 tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc ggggttgagg
    20461 tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag tagccaccac
    20521 gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct gacaacacct
    20581 tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac atctgtcttg
    20641 ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg gattgtttta
    20701 agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc accaatattg
    20761 agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccaggac tgagcccata
    20821 atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa atcgagaacc
    20881 accgttttgc ccttttgctg atgcggcaat accgtcacca gcgccggacg ctcaatcccc
    20941 tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt accggcactg
    21001 acacaggctt gcgctcgacc ttctttcacc aactccaggg ccacgcgcat tgaactccca
    21061 cgactggcgc ggatagcttg cgaaggccgg acatcactgg cgataactga ctgcgcagga
    21121 ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg cgtgatggcg
    21181 tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc ctgcaatgct
    21241 gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc cagggttaga
    21301 cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg ggaaaatcct
    21361 cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg ccgcggtagt
    21421 aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg tctacagaca
    21481 ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg gaacgggttg
    21541 gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc cccgatccaa
    21601 cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg tttggctttt
    21661 gcgcttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca cagtgttcag
    21721 aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc attgcaagca
    21781 gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc agtgcttcag
    21841 cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga tgagtaaacg
    21901 gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg
    21961 cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg tccacactga
    22021 ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg taatcaaggc
    22081 gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt tgcatagggc
    22141 gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt
    22201 tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg ctgataacga
    22261 acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata cgctatatct
    22321 gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc gtcgcgctct
    22381 tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg aaaccccacg
    22441 cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag cgcaatctct
    22501 ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg ttcttgatgg
    22561 cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac gcaaagccag
    22621 cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga atgggcatct
    22681 acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgagcgagg cggagattga
    22741 taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga gtgaaggatt
    22801 tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg ttggtttacc
    22861 gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga tgggatagtt
    22921 gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc tgatgcggcg
    22981 taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat tagcgcgcgt
    23041 tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg atacgcatca
    23101 cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac agacgattta
    23161 atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag cgatcgtcaa
    23221 aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta cgtccggtta
    23281 ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta aagcgtgttt
    23341 ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc agaatatttt
    23401 tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc accagcgcca
    23461 ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc aacgccgagc
    23521 gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga accagttcaa
    23581 ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag cttaaaccac
    23641 tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga tcatcttcat
    23701 ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc gcctcttctt
    23761 cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg agtttatatt
    23821 caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata cggtaaatca
    23881 tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt tgccccgctt
    23941 cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg gtgggcgatt
    24001 ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta gttttacacc
    24061 ttattacttc actacgtgat cactttattg atggttatta aaccaatcac cagcaagaag
    24121 tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta taacaaggct
    24181 tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga aattttacgg
    24241 aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg tattgatctt
    24301 tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat atttctggag
    24361 agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa aaacgggcaa
    24421 ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg cgactagaca
    24481 cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag tcgtcaatgt
    24541 aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca gcaggaagag
    24601 ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga aagtccaggg
    24661 cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga accgagtctg
    24721 gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact aaaagaaatt
    24781 gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat taaagatgtg
    24841 ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg caacaaaggc
    24901 gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat gccgaacaac
    24961 ccgcgcgcgg gtggcatttc tcgccgtatc gaaggcgacg accgtaccga attaaaagaa
    25021 gcactggcga gccttgaact gccggaaggc atggggctta tcgtgcgcac cgctggcgtc
    25081 ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gtctgaaaca ctgggaagcc
    25141 atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga gagcaacgta
    25201 atcgttcgcg cattccgcga ttacttacgt caggacatcg gcgaaatcct tatcgataac
    25261 ccgaaagtgc tcgaactggc acgtcagcat atcgctgcat taggtcgccc ggatttcagc
    25321 agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca gatcgagtcg
    25381 cagatcgaat ccgccttcca gcgtgaagtc cgtctgccgt ccggtggttc cattgttatc
    25441 gacagcaccg aagcgttaac cgccatcgac atcaactccg cacgcgcgac ccgcggcggc
    25501 gatatcgaag aaactgcgtt taacaccaac ctcgaagctg ccgatgagat tgctcgtcag
    25561 ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat gacgcctgta
    25621 cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga ccgtgcgcgt
    25681 attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgtca gcgcctgagc
    25741 ccatcactgg gtgaatccag tcatcacgtt tgtccgcgtt gttctggtac tggcaccgtg
    25801 cgtgacaacg aatcgctgtc gctctctatt ctgcgtctga tcgaagaaga agcgctgaaa
    25861 gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta cctgctgaat
    25921 gaaaaacgtt ctgcggtaaa tgccattgaa actcgtcagg acggtgtgcg ctgtgtaatt
    25981 gtgccaaacg atcagatgga aaccccgcac taccacgtgc tgcgcgtgcg taaaggggaa
    26041 gaaaccccaa ccttaagcta catgctgccg aagctgcatg aagaagcgat ggcgctgccg
    26101 tctgaagaag agttcgctga acgtaagcgt ccggaacaac ctgcgctggc aacctttgcc
    26161 atgccggatg tgccgccagc gccaacgcca gctgaacctg ccgcgcctgt tgtagcccca
    26221 gcaccgaaag ctgcaccggc aacaccagca actcctgcac aacctgggct gttgagccgc
    26281 ttcttcggcg cactgaaagc gctgttcagc ggtagtgaag aaaccaaacc gaccgagcaa
    26341 ccagcaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa gcctcgtcag
    26401 aacaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac tgaaggcagc
    26461 gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcagac tgccgagacg
    26521 cgtgagagcc gtcagcaagc tgaggtaacg gaaaaagcac gtaccaccga cgagcagcaa
    26581 gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc gcaacaagaa
    26641 gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga agaacgtgta
    26701 cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg ttacgagcaa
    26761 agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc tgccgaacca
    26821 attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct gccagtcgta
    26881 gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga caacggtggc
    26941 atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg taagtggtca gcgtcgtcgt
    27001 cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt agcgtgcgcg
    27061 tctccggaac tggcctctgg caaagtctgg atccgctatc caattgtacg tccgcaagat
    27121 gtacaggttg aagagcagcg cgaacaggaa gaagtacatg tgcagccgat ggtgactgag
    27181 gtccctgtcg ccgccgctat cgaaccggtt gttagcgcgc cagttgttga agaagtggcc
    27241 ggtgtcgtag aagcccccgt tcaggttgcc gaaccgcaac cggaagtggt tgaaacgacg
    27301 catcctgaag tgatcgctgc cgcggtaact gaacagccgc aggtgattac cgagtctgat
    27361 gttgtcgtag cccaggaagt tgcagaacaa gcagaaccgg tggttgaacc gcaggaagag
    27421 acggcagata ttgaagaagt tgtcgaaact gctgaggttg tagttgctga acctgaagtt
    27481 gttgctcaac ctgccgcgcc agtagtcgct gaagtcgcag cagaagttga aacggtagct
    27541 gcggtcgaac ctgaggtcac cgttgagcat aaccacgcta ccgcgccaat gacgcgcgct
    27601 ccagcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg ccctactttt
    27661 gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cacatcatgc ctctgccgct
    27721 cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc cctggtaact
    27781 gccagggctt ttttatttca tctttgaatc aacatattaa aattcatcga atggcatcct
    27841 tgctaaccaa caatgcaaaa taggcaaaaa aatgcccgcc ccagcgatcc cagagcaggc
    27901 agacaaaaca tacccagttt catgatatgt tttaaagcga aattatttgt tgagctggaa
    27961 gagcgacaat ccctgcatat cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg
    28021 catgatgtaa gatgaaatag ttgcattcca gtccacatca accagatcgc tcatctgctg
    28081 cgtttgccct aaagcgcgat cgctacctaa tgaatccagc gactccagtt cgttcagctg
    28141 cgtgcctaat tccgcgcgaa cagtcagcac attgttcagc gagtttttca gtccgcggtt
    28201 ggttttatct aacgccgcag cggcggtttc tttatccgct tcgctatccg cgaccggcgt
    28261 tttcagggct gcgatggcac tatccagcat ggcaaaaaga ttggtttcag aagcgctacc
    28321 gtctggttcc gctaccgcgt tgctggtaat actgtcgaaa attttgtcac ccgtgtgccc
    28381 tatcaccatc gaacgcgaag catcgacctg ttgtttaata ctttctgctc caccgacgta
    28441 tttccctttc tcttcgctaa acggcgcagt ctctgtttta taaccggcaa aaatgtagcg
    28501 cccgttaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc cctgaatatc
    28561 cgtagccagc gaggcccggt catcgtcact caaggtgcca ttgctggcgt agacaatttt
    28621 ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt ccagtgacac
    28681 tttttgagtg gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct gtgcctggga
    28741 gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa gggttaacga ctcgcttacc
    28801 cgtcgacatc tgctcgccgt acttcatcca ttctgcctga gaattggtga tgccgcgcat
    28861 gttttgctgg tacatcatct gggtactgaa acgcatttta tctcccctta gcgaatgtta
    28921 atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc caggtaatac
    28981 tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga aatcgactgc
    29041 tgctgattgg aaagctgcgt caccacatta ccttgcgtgg tgctactggt tttcaacgtc
    29101 gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa ggatttcgca
    29161 ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt gcggttgtcg
    29221 ctatcacccg catcttcttc gctcgccatc gcgattttcg cttcgtcggt gattaataca
    29281 tccatgttga cgatggcgtc acttactggt ttcagcgtga agctgtcgtt aacggcaggc
    29341 gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc atctggtgtc
    29401 gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc actgattatt atcgaacgag
    29461 attttgtaat ctgtcgccag taccacggag gcatcagtta ccgtggcacc gatcgcaacg
    29521 tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa gaaatcttca
    29581 ccagcatcgc cgttagcatc aaatccggct ttgtgttggc tgttgaaagc ctcggcaaat
    29641 gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga acggaatgtc
    29701 agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat attgcctgcc
    29761 gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg aactgccgcc
    29821 agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggccatcgt gatgttataa
    29881 gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc gctcaccagt
    29941 tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc
    30001 gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg
    30061 gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta
    30121 aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc
    30181 gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa
    30241 gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcagtcagg
    30301 ccgctacttt gcgtctgcgc agcacgtaac tggttggtaa taaacgcatc atactcacgc
    30361 tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa cgtgctattg
    30421 gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta
    30481 ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattaatc
    30541 aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc
    30601 gccggacttg agttattcag aacagattat caatgttcat actgtaggtt ttgctcacct
    30661 tgtcgcttat cgatttcatc tgctgaatca tgttggtgag tttgcgggca tagtgaggat
    30721 cggtggcata gcccgcgtcc tgtagggcct gcgccccctg ttccgcactc gcggcggtcg
    30781 tcacggcggc gtagcgcggg ttacgcgtta acagcccaac gtaatccgac aaggcttcca
    30841 gatacgagct gtagacgcga aacttcgctt ttactttctt cgcttcgccg ttttcatatt
    30901 cagtcgtggt gatttcagta actggccctt tccagttgcc agaggctttg acaccaaaca
    30961 ggttatagct cggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagattcca
    31021 gtgccgcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg
    31081 cgggcagcga gagttgcgcg aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt
    31141 tacgtggcac ggccttttgc accagctgcg aaagcgcctg attttgataa cgcaccacag
    31201 tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg
    31261 gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt
    31321 gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg ctgaacaggc
    31381 catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca
    31441 cctgacgggc caccggacgg atatttgccg ccggatcttc gcccactttc gcctttagtt
    31501 cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca
    31561 gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca aaatagacat
    31621 cagatccatc ggcgtagcgc ccagcgcatt gagcgcgcgc accacgttat tgaggctggc
    31681 gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg
    31741 agtaaccaca gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg
    31801 attgactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg
    31861 attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac
    31921 attaacctgc atattctgga tatctgcaag gaaacggacc tgggaactgt tgccacttgg
    31981 tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg
    32041 gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt
    32101 aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt
    32161 gatccgtcca ccgttcagtt ggttcacctg aacactgcta ccgccagcgg aggctcctgc
    32221 gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag
    32281 cggcgtcatc aacaacgtac ctccacgcaa gcttttggca tttcccatgg aagaaaccac
    32341 cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca ccattaccgc
    32401 agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag
    32461 catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cggtgccatc
    32521 cagccccacc accagaccat agccaatcag tgagttttgc cttaccccct gaacactggt
    32581 gagatcgcga atacgctcag cctgagccgc cgtcgtgacc agtagaagaa ttaatgcaga
    32641 gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc
    32701 aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatgcgcgca
    32761 tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg
    32821 ccagagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca
    32881 tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta
    32941 ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg
    33001 gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta
    33061 gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc
    33121 aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga
    33181 tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcgga
    33241 ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg
    33301 cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt
    33361 tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttaagcctta
    33421 gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc
    33481 gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt
    33541 ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg
    33601 tgcaccagag gattgcgttt cggtgtagag gttttcgcca atgctctcca gcccggtgtc
    33661 attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg
    33721 ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt
    33781 gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct gatccacctg
    33841 gaaagagccg tcacgggtat aggctgatga accatctggc aacatcacct ggaaaaagcc
    33901 ctgcccttta atcgcgacat ctttgctgtt gttggtctgc gacaggtttc cctggctgtg
    33961 taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt
    34021 ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca gatcttcaaa
    34081 caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt tggcaatgac
    34141 gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat
    34201 aaaatgtcct gtagtggatt agtgacaagc agagcagaag cacgcgacag tgtgaatttc
    34261 ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct tttattgcaa
    34321 tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    34381 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    34441 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    34501 gttggatctg cctgcagtat cggcccacgc gtggcctggc tttctgcgct taaacgaaaa
    34561 atgccgtcgt caccgcgttg cacttcgctg cccgtggctt tcaccagttt cagacgcccc
    34621 actggcgcaa ccgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    34681 gcaatagtga tttccgcccc ttccggcaca gcaattggcc cagcctcgcc tatcaccgga
    34741 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    34801 ccttcgctgc cgtcagcgga ctgcacggcc agccagccat cctgctgcaa tgcaacgtcc
    34861 agcgggcgcg aggtgtaatc catcttgccg ggcgtcatgt ctgcacccgg tgttgacgcc
    34921 gttaccagcg tgcgcgtggg cagtgaaagc ccttccaccg gcactgcacg caaggcgttt
    34981 aactgcgcgc gaaaaccggg cgtagaggca ttggccagat tactggcggt taccgcctgt
    35041 tgattcagcg tctggctggc tgctcccatc gcggtataaa ttgcgtgatc cattgagcta
    35101 tcccgtcagc gattagcgta agttaaccag cgtgttgagg atctggtcct gggttttgat
    35161 ggtctgggcg ttagactgat agttacgctg ggcaacgatc atattgacca gttctttact
    35221 gagatcgacg ttggacgctt ccaacgcacc gttggtcagg gtgccaaagt tgcccgtccc
    35281 ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca gaccagacgt tgtcgccttc
    35341 ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca atctgcccca gcagttgtgt
    35401 ttgttcgttg gaatagttgc cgacaaccgt accgtcatca ttgatttgat aactcaccag
    35461 atcgcccggt ttgtagccat tctgggtggt tgccacaata ttgttagcgc cggtattttg
    35521 ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga tctgcgccgt taattgcgcc
    35581 agtggtaata ttctctgttg gatttgaggt cagaacgcca ttggcattaa acaccagctt
    35641 cattgcaggc tcggctgtac ctgttggatc actgctatcc tgggtgtaga cctgccagtt
    35701 attatccccg gtcttcacaa agtagacgct catgtcatga gcattaccct gactgtcgaa
    35761 aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac
    35821 agtaggaagc ggatcactgg aattcaggtt gatctgcatc gatgccgtgg tggtagtttt
    35881 cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt
    35941 cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat tcaccaggtt
    36001 acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc
    36061 taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt
    36121 ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt
    36181 cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga
    36241 gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac
    36301 cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac
    36361 ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg
    36421 gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt
    36481 actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccgt cagtcaacgt
    36541 accgtcccag gtgaaactgt gaactccggc ggtcagttca ccaatatcaa tggtgcgcac
    36601 aaccgcacca tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc
    36661 aacaccaaac ggcgtggtcg tggtcacagc tccttcttca ctgccggttc ccgcaagaac
    36721 agtggtgccg tggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct
    36781 gttatcaatc tgtccggaaa tagatccgag tgtggtattg agtttttcaa tcccgctgac
    36841 cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc
    36901 ctggtttttc agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat ctgcggcgtt
    36961 gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt
    37021 ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc
    37081 atgcttttca ccgtgttgag cacttcaacg ttggcctgat agctgcgtga cgccgacatg
    37141 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc ctttgcatct
    37201 gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc tataacatcg
    37261 ccaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
    37321 tttgcccgat atggctgtcc atcaggaccg gtcacgctat cagcatttgc cagattactg
    37381 gccgccacgt tcaggcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
    37441 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
    37501 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
    37561 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
    37621 agttctgcgg taggaggcgt cagcgcctgc gccgggatgt gttgcgttga ggtcatcgtc
    37681 agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca
    37741 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
    37801 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
    37861 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
    37921 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatggt
    37981 cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctgacaata
    38041 aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt taagtacggc gagcaatctc
    38101 acatcgcaat tgcacaactt ttttagcgcc caactcgcgg gggtaagtga tgaggttcgt
    38161 gtttctattc gtacagcgcc caatctacta ccgccatgcg agcagccatt gctttcgatg
    38221 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    38281 cgatatttac aggttaatgt gcaggccaca ggaaattatg tggttgccgc gatgcccatt
    38341 gtgcggggag gaaagctgga agctggcaat gtcaaactaa aacgcggacg gctggatacc
    38401 ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccattag cctgcgcgat
    38461 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    38521 ggacaacgcg tcaatgtgat cgccagcggt gatgggttta gcgccaacgc agaaggtcag
    38581 gcgctgaaca atgcagccgt cgcacagaat gcgcgggtgc gcatggtatc gggacaggta
    38641 gtcagcggcg ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat
    38701 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    38761 accatgagta ttgatcgcac ttcgcctctg aagcctgtaa gcaccgttca accgcgcgaa
    38821 accactgacg cgccggtaac gaacagccgg gcggcaaaaa caaccgcctc caccagcacc
    38881 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    38941 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    39001 ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg
    39061 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    39121 ggtaatggat caagagcagc aacatctctc tatggggcag atcaacggca gccagttgca
    39181 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    39241 caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    39301 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    39361 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    39421 tggggcgaac ggtcagacct caacaatcca tcgcggcggt aaaaagattt cgatctgaat
    39481 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    39541 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    39601 cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga
    39661 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    39721 gaaacgccat ccagccgggt tgcggggtaa agattttctg cttacgcaac tgccagtaaa
    39781 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca
    39841 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    39901 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaaaact ttcactacaa
    39961 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    40021 aggcggtaaa tttaccgtac tggaacagcg aaacggtcaa cggacctgaa agaatgccca
    40081 acgcaaccgc actcggcagc gccaacagga aacaaagacg cagcccccag tccatcaaac
    40141 ggttgtattc atcatgattg ccactggcaa aactcttcga cagcgacggc agcaaaatgg
    40201 tgccaagcgc cacgcccagc acaccagacg gaaactccat taagcggtcg gcgtaataca
    40261 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    40321 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    40381 cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    40441 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    40501 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    40561 ctggagcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    40621 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg
    40681 caaatttgtc ggcagtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    40741 cgagcatgcc agcgaccgtc acaaccgcca gcgcgagtgt cagcaggcca gaaacataag
    40801 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    40861 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    40921 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    40981 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    41041 ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc aaggcaggcg
    41101 ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag atgttacagg
    41161 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcacggcc
    41221 tgttcgccgg cggtttgcgg aactgtctga ttttgcacac attcaatgaa gtgccgcgca
    41281 cagccgacaa atccacgttg ttcaagagtg ctctgccaac caggaatcgg tttatgcact
    41341 acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcaccg
    41401 tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaggtggtg
    41461 atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    41521 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    41581 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacactattg
    41641 ctacgatgtt tatccattct tagcgaagct gcggtagcga gttgcgtttt taactcaccg
    41701 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt acgcgccgcc
    41761 agttccacca gccgttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    41821 tgtacacccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggaatgc
    41881 acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    41941 cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc ttgtaacgtc
    42001 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc acctaatccc
    42061 actacgccga tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag
    42121 acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgggcct gtaaatcacc
    42181 atcaaccgcg ttcgacatat ccgtcaccgc cggctgatct tcaacctcac cactgaaaag
    42241 atgcatgtag cggctttcgc gtttacccag ttcgcgggcc agacgcacca caaaaggacc
    42301 atcttcgcga tttgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    42361 atacattcgc gcggcgcagc tgcgcagttc acctggcgtc tgggcaccac gcaataacaa
    42421 cgtggtgatt aacgccactt ccgctgcgct cagtttcaga tcgccaaatt ctgaattaca
    42481 aaaacgttgc tcatatttgg tgacccgatt accaaaaccg ctcactgtgc gtagataatg
    42541 acgtttgacc agattatcca gctgttcctg cacttcggat tcactcagat tcatgaccgg
    42601 ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    42661 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt caagggcagt
    42721 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    42781 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    42841 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    42901 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    42961 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    43021 catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    43081 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    43141 ccagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    43201 gcttgaggaa atggcgattc tctgcgtaat aatccgcaag acgccaggca tcacgatcat
    43261 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    43321 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    43381 atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat
    43441 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt ccccgaagtg
    43501 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    43561 gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata
    43621 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    43681 atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc
    43741 agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga
    43801 ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac
    43861 cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc
    43921 cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgtggtcgt tttttctcgc
    43981 tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg agctggctgt
    44041 tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctgtgtgcgg
    44101 cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacacacg cccgttcgcg
    44161 aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg
    44221 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    44281 ctggcgcacc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    44341 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg
    44401 ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc ggcctgcaac
    44461 aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg
    44521 aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    44581 gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc
    44641 tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt attggcatct
    44701 tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt cgtttgcttg
    44761 aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag
    44821 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    44881 cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg
    44941 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    45001 acagccgcta tatgccagaa agcatggaca tcgttcacta tgtcgataaa ctcgacggca
    45061 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    45121 gctacgcaaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    45181 ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aatttagccg
    45241 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg
    45301 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    45361 tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    45421 ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    45481 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    45541 ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    45601 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    45661 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    45721 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    45781 catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa taaggttacc
    45841 ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg
    45901 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa
    45961 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    46021 ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc tgcttacgtc
    46081 ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa
    46141 gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc
    46201 ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc
    46261 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc
    46321 ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat
    46381 accagcgaaa tttatggacg ggctattgta atgcccaatc tggctccgcc cgtgaccact
    46441 gttgaggctg ccgtggcgta tcgccagcgt attcttgatg ccgtacctgc cgggcacaat
    46501 ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc
    46561 ggatttaacg aaggcgtgtt caccgctgca aaactttatc cggcaaacgc aaccactaac
    46621 tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa
    46681 atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    46741 cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg
    46801 aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat
    46861 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    46921 gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa
    46981 caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct
    47041 gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc
    47101 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    47161 gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa cgacacattc
    47221 atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc
    47281 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    47341 cttcctgttg tcattaggtt attttacctg tataaataac cagtatattc aacagggggc
    47401 tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga
    47461 cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca
    47521 cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa caaaagaaga
    47581 taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt
    47641 cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt tttattttac
    47701 ttacaaattt tgagtacaaa atttctacac ttcctcaaa
//
LOCUS       DAZFWP020000014        47086 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFWP020000014 DAZFWP020000000
VERSION     DAZFWP020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.014"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..264)
                     /locus_tag="ACHK0Z_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             complement(<1..264)
                     /locus_tag="ACHK0Z_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4500832.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSS"
     gene            complement(387..839)
                     /gene="tabA"
                     /locus_tag="ACHK0Z_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             complement(387..839)
                     /gene="tabA"
                     /locus_tag="ACHK0Z_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU4500833.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(984..1577)
                     /gene="bdcR"
                     /locus_tag="ACHK0Z_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             complement(984..1577)
                     /gene="bdcR"
                     /locus_tag="ACHK0Z_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4500834.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            1648..2361
                     /gene="bdcA"
                     /locus_tag="ACHK0Z_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             1648..2361
                     /gene="bdcA"
                     /locus_tag="ACHK0Z_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4500835.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2492..2887
                     /gene="yjgH"
                     /locus_tag="ACHK0Z_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             2492..2887
                     /gene="yjgH"
                     /locus_tag="ACHK0Z_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4500836.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            3168..3302
                     /gene="pyrL"
                     /locus_tag="ACHK0Z_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             3168..3302
                     /gene="pyrL"
                     /locus_tag="ACHK0Z_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU4500837.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3306..4241
                     /gene="pyrB"
                     /locus_tag="ACHK0Z_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             3306..4241
                     /gene="pyrB"
                     /locus_tag="ACHK0Z_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU4500838.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4254..4715
                     /gene="pyrI"
                     /locus_tag="ACHK0Z_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             4254..4715
                     /gene="pyrI"
                     /locus_tag="ACHK0Z_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU4500839.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4788..5174
                     /gene="ridA"
                     /locus_tag="ACHK0Z_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             4788..5174
                     /gene="ridA"
                     /locus_tag="ACHK0Z_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU4500840.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            5240..5695
                     /locus_tag="ACHK0Z_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             5240..5695
                     /locus_tag="ACHK0Z_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4500841.1"
                     /translation="MAKPRFTNEQIAEILQQSKEGASNKELCEHYQFSVSTLRRWQEQ
                     HADGIRSELKKTESKAQIVFLVFFAIAILLTLIFDKPTGGWVIPPLLIYCVYYIRQYR
                     NISGRHIKKEDIYLSRSVNNSYSALYNLSWTFICFFIFAVIYFFIQVFS"
     gene            complement(5740..8436)
                     /gene="mgtA"
                     /locus_tag="ACHK0Z_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             complement(5740..8436)
                     /gene="mgtA"
                     /locus_tag="ACHK0Z_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU4500842.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8577..8630)
                     /gene="mgtL"
                     /locus_tag="ACHK0Z_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     CDS             complement(8577..8630)
                     /gene="mgtL"
                     /locus_tag="ACHK0Z_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU4500843.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8815..9762
                     /gene="treR"
                     /locus_tag="ACHK0Z_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     CDS             8815..9762
                     /gene="treR"
                     /locus_tag="ACHK0Z_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU4500844.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9881..11302
                     /gene="treB"
                     /locus_tag="ACHK0Z_RS03895"
                     /old_locus_tag="pgaptmp_000779"
     CDS             9881..11302
                     /gene="treB"
                     /locus_tag="ACHK0Z_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU4500845.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11352..13007
                     /gene="treC"
                     /locus_tag="ACHK0Z_RS03900"
                     /old_locus_tag="pgaptmp_000780"
     CDS             11352..13007
                     /gene="treC"
                     /locus_tag="ACHK0Z_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU4500846.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKLEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13401..15539
                     /gene="nrdD"
                     /locus_tag="ACHK0Z_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             13401..15539
                     /gene="nrdD"
                     /locus_tag="ACHK0Z_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU4500847.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15697..16161
                     /gene="nrdG"
                     /locus_tag="ACHK0Z_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             15697..16161
                     /gene="nrdG"
                     /locus_tag="ACHK0Z_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU4500848.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QLFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16206..16592)
                     /gene="cybC"
                     /locus_tag="ACHK0Z_RS03915"
                     /old_locus_tag="pgaptmp_000783"
     CDS             complement(16206..16592)
                     /gene="cybC"
                     /locus_tag="ACHK0Z_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4500849.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16775..18127)
                     /gene="pmbA"
                     /locus_tag="ACHK0Z_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             complement(16775..18127)
                     /gene="pmbA"
                     /locus_tag="ACHK0Z_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU4500850.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18221..18772
                     /gene="yjgA"
                     /locus_tag="ACHK0Z_RS03925"
                     /old_locus_tag="pgaptmp_000785"
     CDS             18221..18772
                     /gene="yjgA"
                     /locus_tag="ACHK0Z_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU4500851.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18928..20301)
                     /gene="mpl"
                     /locus_tag="ACHK0Z_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             complement(18928..20301)
                     /gene="mpl"
                     /locus_tag="ACHK0Z_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU4500852.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20477..21475
                     /gene="fbp"
                     /locus_tag="ACHK0Z_RS03935"
                     /old_locus_tag="pgaptmp_000787"
     CDS             20477..21475
                     /gene="fbp"
                     /locus_tag="ACHK0Z_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU4500853.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21508..22503)
                     /gene="yjfF"
                     /locus_tag="ACHK0Z_RS03940"
                     /old_locus_tag="pgaptmp_000788"
     CDS             complement(21508..22503)
                     /gene="yjfF"
                     /locus_tag="ACHK0Z_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU4500854.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22490..23512)
                     /gene="ytfT"
                     /locus_tag="ACHK0Z_RS03945"
                     /old_locus_tag="pgaptmp_000789"
     CDS             complement(22490..23512)
                     /gene="ytfT"
                     /locus_tag="ACHK0Z_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU4500855.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23526..25028)
                     /gene="ytfR"
                     /locus_tag="ACHK0Z_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             complement(23526..25028)
                     /gene="ytfR"
                     /locus_tag="ACHK0Z_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGU4500856.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25168..26124)
                     /gene="ytfQ"
                     /locus_tag="ACHK0Z_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             complement(25168..26124)
                     /gene="ytfQ"
                     /locus_tag="ACHK0Z_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU4500857.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26434..26964
                     /gene="ppa"
                     /locus_tag="ACHK0Z_RS03960"
                     /old_locus_tag="pgaptmp_000792"
     CDS             26434..26964
                     /gene="ppa"
                     /locus_tag="ACHK0Z_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU4500858.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27044..27394)
                     /gene="chpB"
                     /locus_tag="ACHK0Z_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             complement(27044..27394)
                     /gene="chpB"
                     /locus_tag="ACHK0Z_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU4500859.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(27388..27639)
                     /gene="chpS"
                     /locus_tag="ACHK0Z_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             complement(27388..27639)
                     /gene="chpS"
                     /locus_tag="ACHK0Z_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU4500860.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(27852..28193)
                     /gene="ytfP"
                     /locus_tag="ACHK0Z_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             complement(27852..28193)
                     /gene="ytfP"
                     /locus_tag="ACHK0Z_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU4500861.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28196..31975)
                     /gene="tamB"
                     /locus_tag="ACHK0Z_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             complement(28196..31975)
                     /gene="tamB"
                     /locus_tag="ACHK0Z_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU4500862.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAAERWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNCTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPYATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31972..33705)
                     /gene="tamA"
                     /locus_tag="ACHK0Z_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             complement(31972..33705)
                     /gene="tamA"
                     /locus_tag="ACHK0Z_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU4500863.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33911..34549
                     /gene="msrA"
                     /locus_tag="ACHK0Z_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             33911..34549
                     /gene="msrA"
                     /locus_tag="ACHK0Z_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU4500864.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34872..36215
                     /gene="paeA"
                     /locus_tag="ACHK0Z_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             34872..36215
                     /gene="paeA"
                     /locus_tag="ACHK0Z_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU4500865.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36277..36483)
                     /gene="ytfK"
                     /locus_tag="ACHK0Z_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             complement(36277..36483)
                     /gene="ytfK"
                     /locus_tag="ACHK0Z_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU4500866.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36808..37365
                     /gene="ytfJ"
                     /locus_tag="ACHK0Z_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             36808..37365
                     /gene="ytfJ"
                     /locus_tag="ACHK0Z_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4500867.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37355..38095)
                     /gene="cysQ"
                     /locus_tag="ACHK0Z_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             complement(37355..38095)
                     /gene="cysQ"
                     /locus_tag="ACHK0Z_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU4500868.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38285..40228
                     /gene="cpdB"
                     /locus_tag="ACHK0Z_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             38285..40228
                     /gene="cpdB"
                     /locus_tag="ACHK0Z_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU4500869.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40357..40737)
                     /gene="ytfH"
                     /locus_tag="ACHK0Z_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             complement(40357..40737)
                     /gene="ytfH"
                     /locus_tag="ACHK0Z_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU4500870.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40826..41686
                     /gene="qorB"
                     /locus_tag="ACHK0Z_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             40826..41686
                     /gene="qorB"
                     /locus_tag="ACHK0Z_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4500871.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41795..42760
                     /gene="ytfF"
                     /locus_tag="ACHK0Z_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             41795..42760
                     /gene="ytfF"
                     /locus_tag="ACHK0Z_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4500872.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42868..43530
                     /gene="ytfE"
                     /locus_tag="ACHK0Z_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             42868..43530
                     /gene="ytfE"
                     /locus_tag="ACHK0Z_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU4500873.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43575..44987)
                     /gene="cycA"
                     /locus_tag="ACHK0Z_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /gene_synonym="dagA"
     CDS             complement(43575..44987)
                     /gene="cycA"
                     /locus_tag="ACHK0Z_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU4500874.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(45296..45916)
                     /gene="fklB"
                     /locus_tag="ACHK0Z_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             complement(45296..45916)
                     /gene="fklB"
                     /locus_tag="ACHK0Z_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4500875.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            46135..46773
                     /gene="ytfB"
                     /locus_tag="ACHK0Z_RS04050"
                     /old_locus_tag="pgaptmp_000810"
     CDS             46135..46773
                     /gene="ytfB"
                     /locus_tag="ACHK0Z_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU4500876.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46757..>47086)
                     /locus_tag="ACHK0Z_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             complement(46757..>47086)
                     /locus_tag="ACHK0Z_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4500877.1"
                     /translation="IVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFK
                     PDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVD
                     PSSTAHE"
BASE COUNT        11515 a        12583 c        11808 g        11180 t
ORIGIN      
        1 tgaggactta atatgagcca tcatatttct acccgcttcg ttggagcgtt tgctaaaatc
       61 cacataaccc agggtaaata tcttagccaa aaactccaaa aatgctctgg gagaaagaat
      121 attgcttctg ctattctgcc ctccggcaat tcgggcaaaa tcattcattc ccacatacca
      181 ggtatctaag tcttcatttt tttgtttaca attgtcggct gatgtaatgt tttgagaaaa
      241 attccaacct gatattttgc tcatgacgga ctccttgagt taataagaac gtctaagcat
      301 tacaaatttt actcacaaaa aaatatcaaa aaacgctctt ttcacaaact cgccgtaaat
      361 ctggagttat ttacggcgaa aagtcatcac gccatcagca tcttcactac tgctttgcga
      421 acctgggctg gtgcacccac tgcgcacagc ggtttatgca cttcccccgg ataaaacaca
      481 acaaaatcac cttcattaag gataactgtt ttctcatcaa cgccttccgg caaaaatgcg
      541 atgtctttat cagctaacca gtcggtatcc ggcgtgcctg caggttgcgt gctgaaggtc
      601 atgccttcct gaccttttaa cacaatctga atgtcgagat agcgggcatg gtactccgca
      661 cggcgggctt cgtacggctc ggtcatatct tccgagataa gataaaacag acgattgcct
      721 tcgatatcgt gcttgccctt tggcgtttct gccgtaacgt gtgctttgat atgctcaatc
      781 gcctggcgta actcctgggg tagccacggc tgaagattat gaatatttcc gatgatcatc
      841 ttttccactc cttaattaaa acaacgtttc atttctatcg ttatacgaaa agatgatcaa
      901 ttccgctagc cttaacgcgt gaattattgt cttatcacaa cttatcggca ggcgcgttcc
      961 cgcctttcgg atcaaggcca gcatcactcc ttgagaagag tttttatcgc ttcccccgcc
     1021 agtgcagctg ttgcacagag ttgttcgatt gagtgcccct cccgtgcctt cgcagaaagc
     1081 cctgacatca cggtactcat aaaatcagtc acacattgtg cgctttgtgg atgccgcctg
     1141 gcgatatagt cataaatggt cgtttctgcg gcgtgataat attgaacggc aatatcacgc
     1201 gctagtggat catgactatg aataccttca agaaccatac agccagcgca gccgccgttt
     1261 tggctatatc tgcgcgccgc ttcttttaat acctcaacca ggcactcgcc tactggacga
     1321 tcatcacgaa gaatatcggc aagcggaata gcttccgtac cgacgtattc attgagtaca
     1381 cggctaaata acccagcttt actgccaaaa gccgcgtaga ggctcggggg gttaatacca
     1441 agataatcag taacttcagc aacactgaca gcatcgaaac ctttttggtg aaaaagcact
     1501 tttgccgcag agattgcctg ctcaggagcg aaacgtctgg gacgacctgg aacgcgagat
     1561 tgttttttag tgaccattac aaaacctgtt gacagaaagt taaaacagtt ttgtaatgca
     1621 tgttacataa taaatcaagg agtccttatg ggcgctttta caggtaagac agttctcatc
     1681 ctcggtggca gtcgtggtat cggtgccgct atcgtacgtc gtttcgtcac cgatggggcc
     1741 aatgtacgat tcacctatgc ggggtcgaaa gatgccgcta aacgcctggc acaagagact
     1801 ggagcgacag cagtattcac agatagtgct gacagagacg ctgtcattga tgtcgttcgt
     1861 aagagcggcg cattggatat cctggtggta aatgcaggta ttggcgtctt tggcgaggcc
     1921 ctggaattaa atgccgacga tattgatcgc cttttcaaaa tcaatattca tgctccttat
     1981 catgcctctg ttgaagccgc ccggcagatg cccgaaggcg ggcgcatctt aatcatcggc
     2041 tccgtgaatg gcgatcgtat gcctgttgca ggcatggctg cttatgccgc cagcaaatct
     2101 gccctgcaag gcatggcgcg cgggctggcc cgtgattttg gaccgcgtgg gatcaccatt
     2161 aacgtcgtcc agccagggcc aattgatacc gacgctaatc ccgccaacgg gccaatgcgc
     2221 gatatgttgc atagtttgat ggctatcaaa agacatgggc aaccggaaga ggtcgctggt
     2281 atggtcgcat ggttagcagg gccagaagcc agttttgtta ccggcgcgat gcataccatt
     2341 gatggcgcgt ttggcgcata accgactacg ctcaattaag cccagccatt atccatgatg
     2401 tctgggtttt gtttactcac gtcgtccgct aaaagcggct cccggtaaag agaaatcttc
     2461 tgttcatgtc gtaaatcttg aggataacca aatggtagaa agaaccgctg ttttccctgc
     2521 tggccgacat tcactatatg ctgagcatcg ttattctgcg gctattcgtt ccggcgattt
     2581 gctgtttgtt tccgggcaag taggaagtcg agaggacgga acaccagaac ccgattttca
     2641 gcaacaagtc agactggcat ttgataattt gcatgcgacc ctggcagctg cgggatgcac
     2701 ttttgacgat atcattgatg ttacgagctt ccataccgat ccagaaaacc aatttgaaga
     2761 catcatgacg gtgaaaaatg aaatatttag cgccccacct tatccaaact ggacggcggt
     2821 gggtgttaca tggctggcag gctttgattt tgaaattaaa gtgatagcgc gcatccctga
     2881 gcagtaagca atagtgttag ccgttcgctt tcacactccg ccctataagt cggatgaatg
     2941 gaataaaatg catatctgat tgcgtgaaag tgaaaaagaa aaaagcaggg aatgtctgca
     3001 attattgata ccgaacgaca gttcccctgc agaatcacat caaataaaaa tgcatatacc
     3061 ttgactttta attcaaataa accgtttgcg ctgacaaaat attgcatcaa atgcttgcgc
     3121 cgcttctgac gatgagtata atgccggaca atttgccggg aggatgtatg gttcagtgtg
     3181 ttcgacattt tgtcttaccg cgtctgaaaa aagacgctgg cctgccgttt ttcttcccgt
     3241 tgatcaccca ttcccagccc ctcaatcgag gggctttttt ttgcccaggc gtcaggagat
     3301 aaaagatggc taatccgcta tatcagaaac atatcatttc cataaacgac cttagtcgcg
     3361 atgaccttaa tctggtgctg gcgacagcgg cgaaactgaa agcaaacccg caaccagagc
     3421 tgttgaagca caaagtcatt gccagctgtt tcttcgaagc ctctacccgt acccgcctct
     3481 ctttcgaaac atctatgcac cgcctgggtg ccagcgtggt gggcttctcc gacagcgcca
     3541 atacatcact gggtaaaaag ggcgaaacgc tggccgatac catttcggtt atcagcactt
     3601 acgtcgatgc gatagtgatg cgtcatccgc aggaaggtgc ggcgcgcctg gccaccgagt
     3661 tttcaggcaa tgtaccggta ctgaatgccg gtgatggctc caaccaacat ccgacgcaaa
     3721 ccttgctgga cttattcact attcaggaaa cccaggggcg tctggacaat ctccacgtcg
     3781 caatggttgg tgacctgaaa tatggccgca ccgttcactc cctgactcag gcgttagcga
     3841 agttcgacgg caaccgtttt tacttcatcg cgccggacgc gctggcaatg ccgcaataca
     3901 ttctggatat gctcgatgaa aaagggatcg catggagtct gcacagctct attgaagaag
     3961 tgatggcgga agtagacatc ctgtacatga cccgcgtgca aaaagagcgt ctggacccgt
     4021 ccgagtacgc caacgtgaaa gcgcagtttg ttcttcgcgc cagcgatctc cacaacgcca
     4081 aagccaatat gaaagtgctg catccgctgc cgcgtgttga tgagattgcg acggatgttg
     4141 ataaaacgcc acacgcctgg tacttccagc aggcaggcaa cgggattttc gctcgccagg
     4201 cgttactggc actggttctg aatcgcgatc tggtactgta aggggaaata gagatgacac
     4261 acgataataa attgcaggtt gaagctatta aacgcggcac ggtaattgac catatccccg
     4321 cccagatcgg ttttaagctg ttgagtctgt tcaagctgac cgaaacggat cagcgcatca
     4381 ccatcggtct gaacttgcct tctggcgaga tgggccgcaa agatctgatc aaaatcgaaa
     4441 ataccttttt gagtgaagat caggtagatc aactggcatt gtatgcgccg caagccacgg
     4501 ttaaccgtat cgacaactat gaagtggtgg gtaaatcgcg ccccagtctg ccagagcgca
     4561 tcgacaatgt gctggtctgc ccgaacagca actgtatcag ccatgccgaa ccggtttctt
     4621 ccagctttgc cgtgcgaaaa cgcgccaatg acatcgcgct caaatgcaaa tactgtgaaa
     4681 aagagttttc ccataatgtg gtgctggcca attaattgcg gttggtaata aaagtctggc
     4741 tccctataat gagccagact ttttaccgct gtaataaagg agaaatcatg agcaaaacta
     4801 tcgcgacgga aaatgcaccg gcagctatcg gtccttacgt acagggcgtt gatctgggca
     4861 atatgatcat cacctccggt caaatcccgg taaatccgaa aacgggcgaa gtaccggcag
     4921 acgtcgctgc acaggcacgt cagtcgctgg ataacgtaaa agcgatcgtc gaagccgcag
     4981 gcctgaaagt gggcgacatc gttaaaacca ccgtgtttgt gaaagatctg aacgacttcg
     5041 caaccgttaa tgctacctac gaagccttct tcaccgaaca caatgccacc ttcccggcac
     5101 gttcttgcgt tgaagttgcc cgtctgccga aagacgtgaa gattgagatc gaagcgatcg
     5161 ctgttcgtcg ctaatatcta ctgaaagata atccaggtat tgcgcctggg ttatctgatt
     5221 caaccacgga gggttgaaga tggctaaacc ccgtttcact aacgaacaaa ttgctgaaat
     5281 tttacagcag tcaaaagagg gggcgtctaa taaggagcta tgtgaacact atcaatttag
     5341 cgttagcacg ctgcggcgct ggcaagaaca gcacgctgac ggcatcagaa gcgaattaaa
     5401 aaaaacagaa tccaaagcac aaatcgtctt tctggttttc tttgctatcg ctattctact
     5461 gactcttata tttgacaaac ctacaggtgg atgggtaatt ccgcctttat taatctattg
     5521 tgtttattat atccgccagt atcgcaacat ctctggcaga catattaaaa aagaagatat
     5581 ctatctctct cgttcagtaa ataacagcta tagcgccctt tataatttga gctggacgtt
     5641 tatttgtttt tttatttttg cagtaattta tttttttatt caggtgtttt cgtaaaagac
     5701 tgacctgaaa gagtatttca ggccagcctc atcacttgct tactgccagc cgtaacgacg
     5761 gctataaaat cctttcacca actgggttaa cgtcatatac cctgccaaaa tcgcaaccag
     5821 ccacgggaaa tagcttaacg gcagcgcctg cagctgcaga taactggcca gcggtgaaaa
     5881 cggcaatgcg atcccgacaa tcatcacgat cacggtcatg atcattaacg gccacgatgc
     5941 acagctctga ataaacggca cacggcgggt gcggatcata tgcacaatca gcgtttgcga
     6001 cagtaagccc accacaaacc atcccgactg gaacagcgtt tgcgtttccg gcgtgttggc
     6061 atggaatacc caccacatca ggcaaaacgt cagaatatcg aagatcgagc tgatcggtcc
     6121 gaagaagatc ataaagcgcc ccagatccgc cggattccaa cgctgcggct tttgaatttg
     6181 ctcgtcgtcg acgttatcaa acgggatcgc cacctgtgac acatcataca gcaggttctg
     6241 aataagcaag tgtaacggca acatcggcag gaagggcaag aaagcactcg ccaccagcac
     6301 gctgaacaca ttaccgaagt tggagctcgc cgtcattttg atgtatttca gcatgttggc
     6361 gaaagtgcga cgtccttcaa taaccccctc ttccagcacc atcaggcttt tttccagcag
     6421 gatgatatca gccgcttcac gggcaatatc taccgcgccg tccacagaaa tgccgatatc
     6481 cgcagcgcgt aaggcgggcg catcattaat accatcgccc ataaagccaa ccacatgccc
     6541 ttcgcgcttc agtaaggtca caatgcgttc tttatgcatc ggcgtcaggc gggcaaacag
     6601 cgtggtgcgc tgtgcgagat ttgccagttc gtcgtcagat agcgtttcaa tatcactacc
     6661 aatgaccacc tctcccgcgt ccagccccac ttcatggcac actttcgctg caactaactc
     6721 actgtcgcca gtaaggattt ttacggtgat tccactcgct tttaatgcct tcagtgccgg
     6781 agcggttgtc tcttttggcg gatcaaggaa agcaatatat ccttcgagga tcaggtcgga
     6841 ttcatccgcc cgctggtaat ctccttcacg cgctggcagg tatttcgtcg ccaccgcaac
     6901 cacgcgcagc ccctgacgat taagcgtatc agtaacccgc ttaatcttac gcagcatgat
     6961 gtcatcgagc ggcacaatct cgccattgtg acgcacctgc gaacacacat tgaggatttc
     7021 ctgcaatgca cctttgcaaa ccagctgatg gtgctcggta ttttctgcca ctaccaccga
     7081 catccggcga cgctcgaaat caaacggaat ctcatcaatt ttttgccaac gactggccag
     7141 cgagcgcgct gactcttcat ccgtaccttc gagcaccgct gtatcgagca ggtttttaag
     7201 tccggtctga taatgactgt tcaaccacgc gctatgcagc acgcgttcgc tggttttacc
     7261 ggagatatcg gtatgattct ccagcacaat tttgtcctgt gtcagggtgc cagttttatc
     7321 agtgcacaga atatccattg cgccaaagtt ctgaatagca tccagatgtt tgacgatcac
     7381 tttctgtttc gacagcttta ctgcgccgcg cgccagcgtc gaggtaacaa tcatcggcaa
     7441 catttccggc gttaagccta ccgcgaccga aagcgcaaac agtgccgctt cccaccagtc
     7501 gcctttggtg taaccattga ttaacagcac caccggagcc atcaccagca taaagcgaat
     7561 cagcagcata ctgacgcggc tgatcccttg ctgaaaggca ttcggctcgc tttcctgctc
     7621 actaacacgc cccgccagtt gaccaaacca ggtattggca cctgtagcaa tcaccattgc
     7681 ttgtgcagtg ccgctcacca cggtggtgcc cataaaacac agggtgtcac actccagcgg
     7741 attgctgtgc tccggctggc gagtggtagc ggctttttct acgggcagag actcaccggt
     7801 taacgacgcc tgagcgacga acagatcccg cgcctgcaag atacgtaaat ctgccgggat
     7861 catatccccc gccgccagtt ttattatatc gccgggcacc agctggtcga tcgggatctc
     7921 cagccagcca ttttcgcctt tgtcgttaat tacgcgcaga accgtcgctg tattgctgac
     7981 catcgctttc agggcatctg ccgctttagt ggaacgtgct tcctgaataa agttcagcaa
     8041 cgtagaaata gcgaccatta gcgcgataac gcccgcggca aataaatctt ccgtggcgta
     8101 agaaatagcg ccgagaatgg tgagtaaaat attaaagggg ttgcgatagc agacccataa
     8161 atgtacccac cacggcgacg gttgttgtgc gggtaattta ttttcaccat gttgttcgcg
     8221 ggcagattcc acttccgcct gatttaaccc ttccggatgg gtgtcgaacg ttttccataa
     8281 ttcttcttcg ggcatcaccg ccattttcag gcaatgcgca cttaaggacg gcgggaccgc
     8341 cgtattcact gtctgctgcg cgccaggcaa tggatcgcga tgaaccagac gggaaggtaa
     8401 atggcgaatg agccgggtaa aaatttcttt aaacataagg agtccctccg cactgtctga
     8461 aacagtacag aaatgtcttc aggcgtggcg aagccttacc tgaaggtaat aaagctgagc
     8521 agggacgtta aaacgtcacg gccttacgca tccggtaacg ccgtgaaaag acgggcttac
     8581 cggaaaagtt ttaatctccg tcgagggaga ggcgtgggat caggttccat ataacctccg
     8641 gtaagtgaaa aaagcgccgc aaataataag gagaatccaa taatattcgt agcatctatg
     8701 gcgatataat acctgctggc aactaaacca acgataaacc agactttacc cattgctgaa
     8761 tgcacgggta aagttaggct caaataatta aacaacacgt tacaggacaa caggatgcaa
     8821 aatcggctga ccatcaaaga catcgcacgc ttaagcggcg tggggaaatc tacagtttcc
     8881 cgggtgctga ataacgaaag cggcgtgagc cagcgcaccc gcgagcgtgt tgaagcagtg
     8941 atgaatcagc atggattttc cccttcccgc tctgcgcgcg ctatgcgtgg gcaaagcgat
     9001 aaagtggtcg ccatcattgt tacccgcctg gattcgttgt cagaaaatct cgccgttcaa
     9061 accatgctgc cagcgttcta tgaacaaggt tacgacccaa tcatgatgga aagtcagttt
     9121 tccccgcaat tagttgccga acatttgggg gtgctgaaac ggcgtaatat cgacggcgta
     9181 gtgctgttcg gttttactgg cataacagaa gaaatgttag cccactggca gtcatcgctg
     9241 gttctgctgg cgcgtgacgc aaaaggtttt gcttcggtct gttatgacga cgaaggggca
     9301 atcaagatcc tgatgcaacg gctgtatgac caggggcatc gtaatatcag ttatctcggc
     9361 gtgccgcata gtgacgtgac gaccgggaag cgacgtcacg aagcctacct ggcgttctgc
     9421 aaagcgcata aactgaatcc cgttgccgct ctgccaggcc ttgctatgaa gcaaggctat
     9481 gagaacgtag caaaagtgat tacgcctgaa actaccgcct tactgtgcgc aaccgacacg
     9541 ctggcacttg gcgcaagtaa atacctgcaa gagcaacgca tcgacacctt gcaactggcg
     9601 agcgtcggta atacgccatt aatgaaattc cttcatccgg agatcgtcac cgtagatcct
     9661 ggttacgccg aagctggacg ccaggcggcc tgccaattga tcgcacaggt aaccgggcgc
     9721 agcgaaccgc aacaaatcat catccccgcc accctgtcct gatcgtttcc tgaacgataa
     9781 attgtgatct tcgctgcgtt tcgggaacgt tcccgttttt aaatttttcc gcgcaatata
     9841 ttctgcagcc aaccaaaaat gtcatctgcc atggggcttt atgatgagca aaataaacca
     9901 aacggatatc gatcggttga ttgaactggt cggcgggcgc ggcaatattg cgacggtgag
     9961 ccactgtatt actcgcctgc gctttgtcct caaccaaccg gccaatgcca gaccgaaaga
    10021 aattgagcaa ctccccatgg tgaaaggctg tttcaccaat gccgggcaat ttcaggtggt
    10081 gattggcacc aacgtgggtg attactatca agcactaata gcgtcaaccg gacaggcgca
    10141 ggttgataaa gagcaggtaa aaaaagccgc ccggcagaat atgaaatggc atgagcagtt
    10201 gatctctcat ttcgcggaga tcttcttccc gttgctgccc gcgttgatta gcggcggttt
    10261 gatcctcggt tttcgtaatg tgatcggcga tttgcccatg agcaacggtc aaacgctggc
    10321 gcaaatgtac ccttccctgc aaacgatcta cgattttctg tggttgatcg gtgaagcgat
    10381 cttcttctac ctgccggtcg ggatttgctg gtcagcggtg aaaaaaatgg gcggcacgcc
    10441 gatccttggt atcgtgcttg gcgtgacact ggtttccccc cagctgatga acgcttatct
    10501 gctcgggcag cagctgccgg aagtgtggga ctttggcatg ttcagcatcg ccaaagtggg
    10561 ctatcaggcg caggtgatcc cggcactgtt agccgggctg gcgctgggcg ttattgaaac
    10621 tcgccttaaa cgcatcgtac cggattacct ctatctggtg gtggtgcccg tctgttcgct
    10681 gatcctcgcg gtgttcctcg cccatgcgct gattggtccg tttggtcgca tgattggcga
    10741 tggcgttgcc tttgcggtac gtcacctgat gaccggcagc tttgctccga ttggtgcggc
    10801 attgtttggc ttcctgtacg cgccgctggt gatcaccggc gtacaccaga ccacccttgc
    10861 tattgatttg cagatgattc aaagcatggg tggcacgcca gtgtggccgc tgattgcgct
    10921 gtcgaatatc gctcagggct ccgccgtgat aggcattatc atttccagcc gcaagcacaa
    10981 tgaacgcgag atctccgtgc ctgccgctat ctccgcctgg cttggggtca ctgagcctgc
    11041 aatgtacggc atcaacctga aatatcgctt cccgatgctg tgcgcgatga ttggttctgg
    11101 tctggcagga ttactatgcg gcctgaacgg cgttatggcg aatggtatcg gcgtaggcgg
    11161 cctgccggga attctctcga ttcaaccgag ctactggcag gtatttgcgc tggcaatggt
    11221 tatcgccatc atcatcccga ttgtactcac ctcgtttatc tatcagcgga aataccgcct
    11281 gggcacgctg gatattgttt aattttcttc ggggcgcaat tgcgccccct cgcatttgca
    11341 ggaataacgt aatgactaat cttccccact ggtggcaaaa cggcgttatc taccagattt
    11401 atccaaagag ttttcaggac accacgggta gcggcaccgg cgatttacgt ggcgttatcc
    11461 aacgcctgga ctatctgcat aaactgggcg ttgatgccat ctggctaacc cccttttata
    11521 tctctcccca ggtcgataac ggttacgacg tagcgaacta tacggcgatt gatcccacct
    11581 acggcacgct ggacgatttt gacgaactgg tgacgcaggc aaaatcgcgc gggattcgta
    11641 tcattctcga tatggtgttt aaccatacct ctacccaaca tgcctggttt cgcgaggcgc
    11701 tgaacaaaga aagcccttac cgccagtttt atatctggcg cgatggcgaa ccagaaacgc
    11761 caccgaacaa ctggcgttca aaatttggcg gtagtgcgtg gcgctggcat gcggaaagcg
    11821 aacagtacta tttgcatctc tttgcaccag aacaggcgga tctcaactgg gagaatccag
    11881 cggtacgcgc agagctgaaa aaagtctgtg aattctgggc cgatcgtggg gtcgacgggt
    11941 tgcgcctgga tgtggtgaat ctgatctcca aagacccgcg tttccctgaa gacctggatg
    12001 gcgacgggcg tcgcttttac accgacgggc cacgagcaca cgagtttttg cacgagatga
    12061 accgcgatgt gtttacgcca cgcgggttaa tgaccgtagg tgaaatgtcc tccaccagcc
    12121 ttgagcattg ccagcgatac gcggcactga caggcagtga attgtcgatg acctttaatt
    12181 ttcatcacct gaaggtcgat tatcccggtg gtgaaaaatg gacgctggct aaacctgact
    12241 ttgtggcgtt gaaaacattg ttccgccact ggcaacaagg aatgcacaac gtggcatgga
    12301 atgccttgtt ctggtgtaac cacgatcagc cgcgcattat ttctcgcttt ggtgatgaag
    12361 gtgaataccg cgtgcctgcg gcaaaaatgc tggcgatggt gctgcatggc atgcagggaa
    12421 cgccgtatat ctaccaaggc gaagagattg gcatgaccaa cccgcatttc acgcgcatta
    12481 ctgactatcg cgacgtggag agcctcaata tgtttgccga gctgcgcaac gatggtcgtg
    12541 atgccgacga gttattggca atcctcgcca gtaaatcccg tgacaacagc cgcacgccca
    12601 tgcaatggag caacggcgat aatgccgggt ttacggctgg cgaaccgtgg attggcctgg
    12661 gcgataacta tcaacaaatc aacgtagaag ccgcgctggc cgatgactcc tcggtgtttt
    12721 acacctacca aaagttaatc gcactgcgta agctagaagc catcctgaca tggggcaatt
    12781 accaggatct gctgccaaac agccctgtat tgtggtgcta tcgccgtgaa tggaaggggc
    12841 aaaccttgct ggtcattgcc aaccttagcc gtgagatcca accctggcag ccagggcaaa
    12901 tgcgcggcaa ctggcagctt gtgatgcata actacgaaga agcctcacca caaccctgtg
    12961 ccatgacttt acggcctttt gaggctgtct ggtggttaca gaagtaagta tccgctaagc
    13021 cccgggaatg cctgtcattc cggggttttg tctgtcagca tgttgttttt gttgattttt
    13081 caaccagcaa attcattaaa aaatttacat aacgctgtag cgcccgtcat ccgtacgctc
    13141 tgctttttac tttgagctac atcaaaaaaa gttcaaacat ccttgatgca aagcactata
    13201 tatagacttt aaaatgcgtc ccaacccaat atgttgtatt aatcgactat aattgctact
    13261 acagctcccc acgaaaaagg tgcggcgttg tggataagcg gatggcgatt gcggaaagca
    13321 ccggaaaacg aaacgaaaaa accggaaaac gcctttccca atttctgtgg ataacctgtt
    13381 cttaaaaata tggagcgatc atgacaccgc atgtgatgaa acgagacggc tgcaaagtgc
    13441 cgtttaaatc agagcgcatc aaagaagcga ttctgcgtgc agctaaagca gcggaagtcg
    13501 atgatgccga ttattgcgcc actgttgccg cggttgtcag cgagcagatg cagggccgca
    13561 accaggtgga tatcaacgag atccagaccg cagttgaaaa ccagctgatg tcgggtccat
    13621 acaaacaact ggctcgtgct tacatcgagt accgtcacga tcgcgacatt gaacgtgaaa
    13681 aacgcggtcg cctgaaccag gagatccgtg gtctggtcga gcagaccaac gcctcgttac
    13741 tcaacgaaaa cgccaacaaa gacagcaagg tgattccaac ccagcgcgac ctgctggccg
    13801 ggatcgtggc taaacactat gcacgccagc acctgctgcc gcgtgacgtg gtgcaggcac
    13861 atgagcgtgg cgatattcac tatcacgatc tcgattactc gccgttcttc ccgatgttca
    13921 actgcatgtt gatcgacctg aaaggcatgc tgacccaggg ctttaaaatg gggaacgccg
    13981 agattgaacc gccgaagtcg atctctacgg caaccgcggt aactgcgcag attattgctc
    14041 aggtcgccag ccatatttat ggcggcacca ccattaaccg tatcgatgaa gtgctggcgc
    14101 cgtttgttac cgccagctac aacaaacatc gcaagaccgc agaagagtgg aacatcccgg
    14161 acgccgaagg ctacgcaaac tctcgtacca tcaaagagtg ctacgatgcc ttccagtcgc
    14221 tggagtacga agtaaacacc ctgcacaccg ccaacggtca gacgccgttt gtaacctttg
    14281 gttttggtct gggcaccagc tgggaatcgc gcctgattca ggaatcgatc ctgcgtaacc
    14341 gtatcgcagg cctcggtaaa aaccgtaaaa ctgcggtgtt cccgaaactg gtgtttgcga
    14401 ttcgcgatgg cctgaaccat aaaaaaggcg atccgaacta cgacatcaaa cagctggcgc
    14461 tggagtgcgc aagcaagcgc atgtatccgg atatcctgaa ctacgatcag gtagtgaaag
    14521 tcaccggttc gtttaaaacc ccgatgggct gccgcagctt cctcggcgtg tgggaaaatg
    14581 aaaacggcga gcagatccac gatggtcgta acaacctcgg cgtgatcagc ctgaacctgc
    14641 cgcgtattgc tctggaagca aaaggcgatg aagccacctt ctggaagctg ctggatgaac
    14701 gtctggtgct ggcacgtaag gcgctgatga cccgtatcgc tcgtctcgaa ggcgtgaaag
    14761 cgcgcgtggc cccgatcctc tatatggaag gtgcttgtgg cgtgcgtctg aatgctgacg
    14821 atgatgtttc tgaaatcttc aaaaacggtc gtgcgtctat ttcgctgggt tacatcggca
    14881 tccacgaaac cattaacgcg ctgttcggcg gcgagcatgt atatgacaac gagcagcttc
    14941 gcgcgaaagg tatcgccatt gttgaacgtc tgcgtcaggc agtggatcag tggaaagaag
    15001 aaacgggtta tggtttcagt ctctacagca cgccgagtga aaacctgtgc gaccgcttct
    15061 gccgtctcga tactgctgag tttggcgtgg tgccgggcgt gaccgataaa ggttactaca
    15121 ccaacagttt ccacctcgat gtggagaaga aggtgaaccc gtacgacaag atcgactttg
    15181 aagcgcctta cccgccgctg gcgaacggtg gtttcatttg ctacggcgag tatccaaaca
    15241 ttcagcacaa cctgaaggcg ctggaagatg tctgggatta cagctatcag catgtaccgt
    15301 attacggcac caatacaccg attgatgagt gctacgagtg tggctttacc ggtgagttcg
    15361 agtgcaccag caaaggcttc acttgcccga aatgtggtaa ccatgacgcc tcccgtgtgt
    15421 cggtaactcg ccgcgtgtgc ggatatttag gtagcccgga tgcacgtccg tttaacgctg
    15481 gtaagcagga agaagttaag cgccgcgtta aacatttggg gaatgggcag ataggttaat
    15541 cttcactctg cgctaagcaa atgccggatg cggcgtaaac gccttatccg gcctacattt
    15601 ggcaacggct gtaggcctga taagacgcga cagcgtcgca tcaggcattg attaccggat
    15661 gcagcctaca cgcggactga taagccaggc gaaataatga attatcatca gtactatcct
    15721 gtcgacatcg tcaacggccc cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat
    15781 gaatgccccg gttgctataa caaaagcaca tggcgggtaa attctggtca gctatttacc
    15841 aaagcaatgg aagaccagat cattaacgat ctgaatgaca ctcgtatcaa acgccagggt
    15901 atttccctct ccggcggcga tccgctgcat ccgcaaaacg tgccggatat tctgaaactg
    15961 gtacaacgca tccgcgccga gtgtccgggt aaagacatct gggtgtggac aggctataaa
    16021 ctcgacgaac tcaacgctac gcaaatgcag gttgttgatt tgattaacgt gctggtcgac
    16081 ggcaagtttg tgcaggattt aaaagaccct tccctgatct ggcgcggcag cagcaatcag
    16141 gtggtgcatc atttgcgatg ataagttccc tgttacctga gtgcaggcaa cagggaaatg
    16201 aggaattaac gatacttctg gtgataggcg ttgcgggtcg ttttcagttg ctctgcggcg
    16261 gcctgcgctt cttttacttt accttcattt gccagcttca gcgcgtcgtc aatctgaccg
    16321 accagaatgt cgaaaccgtg gcggaaatct ttcatttccg ggctgtccgg tgatttatct
    16381 tcgagcttcg gcggcgttgc tttttgcgca tccagcgctg cggcgcgcat catcgttaac
    16441 gcgtctttga cttgcgccgc gttatccgct ttttcgatca cttttaaatt gtcgttgagg
    16501 gtttccatat tgtcttcaag atcagcggca aacgacgcag aactgaatac caacgaggag
    16561 actgcaagaa tagctaacag gcttttacgc attgctcact tcctttttta tttacacttc
    16621 atccttcaca ctgcctcatc gacggctacg ctcattcgcc cgggtcacgt actgcgagta
    16681 cgctccccgg ccctcattca ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt
    16741 tttagtttat agcgcggcag gtcgcgccag ttttttactg cccggcgatt ttcatctccg
    16801 gcaacagcac agaaccacac tgtatattac tgcgtgtttc aatatcgtta ccgacggtga
    16861 caatattgcg ccacatatct tttaaattac ctgcgatggt gatttcacta accggatact
    16921 gaatttcgcc attctctacc cagaagcccg cagcaccacg ggaataatca ccagtaatgg
    16981 cactcacgcc ctggcccatc agctccgtca ccaccagccc ggtgcccatc tctttgagca
    17041 tttgttcgaa gcttagccct tgtccggcaa tccgccagtt gtgaataccg cccgcatgtc
    17101 cggtgctttt cagccccagt ttccgcgccg agtagctggt cagcagccac tgagtcagga
    17161 tgccatcttt aataatatcg cgacgctcgg tgcgcacacc ttcgctgtcg aatggcgtcg
    17221 acgccagccc tttcagcaga tgcggatgct cttcaatggt cagccagtcc ggcagaattt
    17281 gtttacccag cgaatccagc agaaaggtag atttacggta aaccgagcca cccgctatcg
    17341 cccccaccag atggccaaaa agcccggttg ccacttcatt ggcaaaaatg actggcgctt
    17401 tcatggtgga gagtttacgc ggtgacaggc gcgataaagt acggcgagca cagtcggccc
    17461 caacccactc tggcgtttgc agatcgctca tcgcacgacc aatggtgtag gcgtaatcac
    17521 gctccatatc gccattttct tcggcaatta cacagctgga gagtgaatga cgcgtagagc
    17581 agtaaccctg caacatgccg tggctgttgc caaaaacttt gacaccgtag tggctgttaa
    17641 aactgccgcc ttcggtattg gtaatgcgtt tgtccgcctg caatgccgcc tgttctgcgc
    17701 gggccgccag ttcaatggca tcatccgggg aaacttcagc agggtggaac aagtcgagat
    17761 ctggtgcgtc aaaggccagc agctctttgt ctgccaggcc ggcacaggga tctggcgagg
    17821 tataacgggc aatatccagc gccgcctgta cagtacgggc aatggcctgc gggcttaaat
    17881 cggtggatga tgcgctacct ttgcggttct ggtgataaac agtgatcccc agcgcgccat
    17941 cgctattgaa ttcaacattc tccacttcac cataacgcgt gcttacgcta atgccggtgg
    18001 tcttgctgac ggcaacttcc gcaccgtccg atttgcctga ggccaactcc agcgcagtgg
    18061 aaactgcttc ttccagaatc ttgcgctgcg cttcaacttg agagattact ttcattgcaa
    18121 gtgccataat gtagagagtt tctctgaagt ctaacagaga accgtttttc agtgcgcatc
    18181 ttaactggta acattagcct ctttttttaa ggagcctgag atgactaagc agcccgaaga
    18241 ctggctcgac gacgttcccg gtgatgacat cgaagacgaa gacgatgaaa ttatctgggt
    18301 cagtaaaagt gaaattaaac gtgatgccga ggagctaaaa cgccttggcg cggaaattgt
    18361 tgatctgggg aaaaacgcgc tggataagat cccgttagat gcggatctgc gcgcggctat
    18421 tgagctcgcc cagcgtatta agatggaagg tcgccgccgc cagttgcagc tcatcggtaa
    18481 gatgctgcgc cagcgcgacg tagagcctat tcgtcaggcg ctggataagc tgaaaaatcg
    18541 ccacaatcag caggtggtgc tgttccataa actggaaaac ctgcgcgatc gtctgatcga
    18601 tcaaggtgat gacgccatcg ctgaagtatt gaatctgtgg cccgatgccg atcgtcagca
    18661 gctgcgtact ctgatccgta acgcgaagaa agagaaagaa gggaataagc cgccgaaatc
    18721 cgcacgccag attttccagt atctgcgcga actggcggag aatgaagggt aattttcgtt
    18781 gttgatgtga gtatggtttg ccggatgcgg tgcacctgta ggcctgataa gacgcatcaa
    18841 gcgtcgcatc aggcactatg ctcaaatgcc ggatgcggcg tgaacgcctt atccggccta
    18901 cgcacactat atcaggctga ggccgaatta ctgcgcggct tccgccttct tcgccagacc
    18961 atccagcagt ttctgatgga tgccaccaaa accgccgttg ctcatgacca gaatatggtc
    19021 gccaggctga gcggttttca ccaccatatc tgccagcgta tccacatcgc cactccagtg
    19081 tgcaggctga acgcaggctt ctgccacttc tgccacctgc cacggaatat gcgccggttg
    19141 cagcaggaag acttcatcgg cacgacctaa tgaaggtgcc agatcgtctt tgcagatccc
    19201 cattttcatg gtatttgagc gcggttccag cacagcaata atgcgcgccg taccaccaac
    19261 tttgccacgc agcgccgcca gcgttgccag aatcgccgtc gggtgatggg caaaatcgtc
    19321 atataccgtc acgccattcg cttcaccacg caactccaga cggcggcgag cgttaataaa
    19381 cgaacccagc gcgttagcgg catctgcagg cgctacacca acatggcgag ccgctgcaat
    19441 cgccatcagg ccattgtgca tattatgttc gcctaccagc gaccatttca cttcgcccac
    19501 tttttcgcca tcaagcaaga cttcccattc ggaagcatcg gtggtcagct ttttcgcctg
    19561 ccagtgcccc tgctcaccca ccagctcctg ctcgctccag cagcccatcg ccatggtctg
    19621 tttcaggttg atgtcatttt ccggccagat aatacggccc tgccccggaa cgatacgcac
    19681 cagatggtgg aactgtttct ggatcgcttt caggtcgtca aagatatcgg cgtgatcgaa
    19741 ctcaaggttg ttgaggatca gcgtacgcgg gcagtaatgg acaaatttag agcgtttgtc
    19801 gaagaaggcg cagtcgtact catccgcttc gataacaaag aagtcgcttt cgcccagacg
    19861 cgccgaaacc tcaaagttcc ccggcacacc gccgattaca aagcccggtt tgtaaccaca
    19921 ctgttccaga atccaggtcg ccattcccgc ggtggtggtt ttgccgtgtg tacctgcaac
    19981 ggccagcacc cagcggtcgc gcagcacaaa atcgtgcagc cactgtggac ctgacatata
    20041 agggatgttt ttttccagta ccgcttccac acacggattt ccacgggtca tggcgttgcc
    20101 aataatcacc agatccggct gcggatcgag ctggctggca tcgtaaccct gaatcagttc
    20161 aatgccttgc ttctcaagta aggtgctcat cggcggatac acattggcgt ccgaacccgt
    20221 tacttcatgg cctaactggc gcgccagcat cgccagaccg cccataaacg tgccacaaat
    20281 tcctaaaata tgaatgcgca tacgtcacta tcctttttta atctgccgct cattttacgc
    20341 atatgtcggg caagtgagaa acgcatttca ggataatccg caatttgctg gcgcgattca
    20401 cctgagcgca acattccgat tatttgttaa gattgttgcg gtcgctttac tccataaaca
    20461 ttgcagggaa agttttatga aaacgttagg tgaatttatt gtcgaaaagc agcacgagtt
    20521 ttctcatgct accggtgagc tcactgcttt gctgtcggca ataaaactgg gcgccaagat
    20581 tatccatcgc gatatcaaca aagcaggact ggttgatatc ctgggtgcca gcggtgctga
    20641 gaacgtgcag ggcgaggttc agcaaaaact cgacttgttc gctaatgaaa aactgaaagc
    20701 cgcactgaaa gcacgcgata tcgttgcggg cattgcctct gaagaagaag atgagattgt
    20761 cgtctttgaa ggctgtgaac atgcaaaata cgtggtgctg atggacccac tggatggctc
    20821 gtccaacatc gatgttaacg tctctgtcgg taccattttc tccatctacc gccgcgttac
    20881 gcccgtcggc acgccggtaa cggaagaaga tttcctccag cctggtaaca aacaggttgc
    20941 ggcaggttac gtggtatacg gctcctctac catgctggtt tacaccaccg gatgcggtgt
    21001 tcacgccttt acttacgatc cttcgctcgg cgttttctgc ctgtgccagg aacggatgcg
    21061 cttcccggag aaaggcaaaa cctactccat caacgaagga aactacatta agtttccgaa
    21121 cggggtgaaa aagtacatta aattctgcca ggaagaagat aagtccacca accgcccgta
    21181 tacctcacgt tatatcggtt cactggtcgc ggatttccac cgtaacctgc tgaaaggcgg
    21241 tatttatctc tacccaagca ccgccagcca cccggacggt aaactgcgtt tactgtatga
    21301 gtgcaacccg atggcattcc tggcggaaca agcgggcggt aaagcgagcg atggcaaaga
    21361 gcgtattctg gatatcatcc cggaaaccct gcaccagcgc cgttcattct ttgtcggcaa
    21421 cgaccatatg gttgaagatg tcgaacgctt tatccgtgaa ttcccggacg cgtaatctcc
    21481 tgcgcctctc cacacggaga ggcgttttta tcgctgcgca atgttgactc ttgtcactgg
    21541 cgaactctga cgattctccc acagcaccgt taatccacgc tgtaatgcta taaaaataaa
    21601 caacaaaata ccgatggcga ttttcgtcca ccatgagctc agcgtgccat caaagtttat
    21661 gtaagtctga atcagtccct gaatcgccac gccaaaaagc gtccctaata ccgttccaac
    21721 gccaccgctc aaaagcgtac cgccaattac cactgaggcg atagcatcca gttccacacc
    21781 tacgcccgcc agcgcatatc cggcctgggt ataaatcgag aagacaatcc ccgccagcgt
    21841 tgccagtccg gtggagagca tataaatgcg aatagtggtg ctgcgggtag aaatccccat
    21901 caggtttgcc gacgttgcgt tgccgccaat ggcgtatacc tgattaccaa aacgggtacg
    21961 atgcgcgagg aatatgccga taaccaccac cgccagcatc agcagtccca tcgcacttaa
    22021 gcgaccgccg ccagggattt tccacgcaag gcttgagagc gtgtcataaa tgggatggtt
    22081 tatcggaatc gactcttccg aaacgagata gctgacgccg cgcaaaaaga acattcctgc
    22141 caaagtaatg ataaacgccg ggatcttcag ggcgtcgatc aaaagcccca taaatgcgcc
    22201 aaaggcacag cccattacca gcaccagcgg aaatgccagc aacggcgaga ggccgaagtc
    22261 gccaatcact tttgccagaa acacgccggt aaaagcgatc accgaaccga cggagagatc
    22321 gatcccaccg gagaggatca caaaggtcat gccaacggca atgatcccaa gaaaggcgtt
    22381 atcggtcagg atattgcaga tcactcttgt ggaagcaaaa ccgggaaact gcgtcaggca
    22441 gtagagatag cccagcacaa agacgccgat ggtgatcatc agtggcaaat tacgttttat
    22501 cacggctacg tactccttta atcagactga taaagcgttg cgactggaca atcagcacac
    22561 aaagcaccac caccgctttt acgacctggt tcatctccgg cggaaagcca gaaagtaaaa
    22621 ttccggtatt cattccctga ataatcagcg cccccaccac cgaaagtagc aggttaaagc
    22681 gcccgcccat cagcgatccg ccgccaatca ccaccgcgag aatggcgtcc agctccagcc
    22741 ataacccggc gttattggca tcggcaccgc gaatatccgc cgccacgata atgcccgcaa
    22801 tcgccgcaca tagcccgctc aacacgtagg tgagcatgac gatgattcgc gtgtttaccc
    22861 cggcattttt tgccgcccga atgttgatac caacggcttc gataaacatc cccagcgccg
    22921 ttttgcgggt caacagccag aacaggagaa gcgtcagcac cgcgataatg accggcgttg
    22981 gcaggaacaa cagcgatccg ctgccaaacc atgagagatc cggcgagtta aacgtgacga
    23041 tctggccgga ggtgatcagt tgcgccacgc cgcgcccggc gaccatcagg atcagggtgg
    23101 cgacaaacgg ctgaattttg aggatcgcta ccagtatgcc gttccacaat cccgccagga
    23161 tgccggtgcc cagggcgctt aacaaaacaa tcggcaggct gaatcccgcg accgtcatcg
    23221 cagccgttgt ggctccggcg atagccatca ccgcccctac ggagagatca atcccaccgg
    23281 tggcgatcac cagcgtcata ccaatcgcca gtaacgcaac gggagctgca cggttaagaa
    23341 tgtcgatagg gctaccgaac aaacgcccat cctgaagcac cacctgccag aaatgcgggg
    23401 ctaccaggct atcgaccagc agcaccagca atagcgccgc cagctgcgac attcccgttg
    23461 gccagcgaaa gcgccttttc ggcggcgtag tgtccgggag agattgaggc atcacactgt
    23521 tctccttacg ccgcaatggc gttcatgatc gccggaacgg aaagctctgc cagcgggatc
    23581 tccgccacct gtttgcgatc gcgcatgata atcacccgat cggcatagcc caccagctct
    23641 tccagttcag aggagatcac cagcagcgcc agaccatcgg cgcatagcgt ttcaatcagg
    23701 cggatgatct cggcgtgggc acccacatca atgccgcggg ttggctcatc gaggatcaga
    23761 aattgcggtc gggtcagtag ccaacgtgaa agcaacactt tttgctgatt gccgccagag
    23821 agaaattcaa tcggttgttc agttgaaggt gtgcgaatgc caagctggcg gataaagcgt
    23881 tcggcaatct cttgctgctc tttgcgggaa atgggtcgta gccagccgcg ctgggcctgg
    23941 agagcgagga tgatattttc ccgcaccgag gcggcggcga tgatgccatc ggttttcctg
    24001 tcttccgggc agaatccgat gcccagtact gaagcctgat gtggcgatcg cagggtttgc
    24061 ggtttgcctt tgatcaacgc cgtgccgctg tcagcaggtt tgataccgaa gatcacttcg
    24121 gcggtttcgg tacgtccgga tcccagcaag ccagccagac cgacgatctc gccggggcgc
    24181 acttcgagat caaacggtgc tatcgttcct tttttgccgt aatttttgaa cgcggcaacg
    24241 ggtttgtcgc tcaacaatgt tcgcccggca cgctgtagcg cgtgggtatc cagctcgcgc
    24301 cccagcatca tttttaccag ttcgatctgc ggtagctcgc gcgtttcccg acagcctacg
    24361 aaactgccgt tgcgtaagac agtgatccga tcgctgacct gatagacctg atcgagaaag
    24421 tgagtgacaa agatcaggct gacgccgcga tcgcgcaact gacgcatcag gccaaataat
    24481 agctccactt cctgggtgtc gaggctggcg gtgggttcat cgaggatcag cactttggcg
    24541 gagagatcga tagcccggca aatggcgacg atttgctgca tcgcgactga aaagcggtta
    24601 agcggttcgc gcacgtcgag gaagaaaccg taagatgcca tcagttcggt ggcgcgcttt
    24661 tccatctctt tacggcgtag aaggccgaaa cgtttgggtt cgcgacctat gaatagatta
    24721 tcagcaaccg acatattggg tagcaggttg acttcctgat agacggtgcc gataccgagt
    24781 tgttgtgcat gggcggtatt tttcggtgag atagcctggc cttccagcca gatggtgccg
    24841 cgatcggcgt ggtatacacc agttaatgct ttgattagcg ttgattttcc cgccccgttt
    24901 tcaccaagca gcgccatgat ttcgccacgg cgcaggctga aatcaacgtt gtctaacgct
    24961 ttgacgccgg ggaaaaattt acttaatcct tcggtgcgga ggatctcctg gtgttggtcg
    25021 gtcgtcatgg tttcccccgg tgtaaagcgt gctcccagta ggcatgataa gacgcggtaa
    25081 gcgtcgcatc aggcttcagc gcaccaccgc cggatgcggc gcgagcgcct tatccggcct
    25141 acgaatcggc atcaggcata gcaaccctca ataccccata tttttcttct tctctaactc
    25201 ttcttttgcg gtatcaggca gataaagggt ggatttggtc agggtcagct tttcaggcat
    25261 ggtgccgtct tttttgtatt tctccagcgc atcgaaagcg ggacctgcca tattcggcgt
    25321 cagttcaaca ctggcgttcg cttcgccatc aatcatcgct ttgtagatat ccggtacacc
    25381 gtcaatggaa cccgtgagga tatctttgcc cggtttcagg cccgcttctt taattgcctg
    25441 aattgcgcca atcaccatgt catcgttatg ggcgtaaacc atgcagatgt ttttgccgtt
    25501 gttttccgct ttgataaagc tctccatgac ttctttgcct ttactgcggg tgaagtcacc
    25561 tgactgcgag cggataattt tgatatttgg cgcattctta atggcttcgg caaagccttt
    25621 cttacggtca atggcgacgc tggccccaac ggtgccctgc aactccacca cgttgcatgg
    25681 tttgccattc acttctttta ccagccagtc accgatcaac ttaccttcga ggatgttgtc
    25741 ggcagtaacg gtggtcatat agagcgattt gtctttcaca tcgatggaac ggtcgagcaa
    25801 gaagaccggg atttcggcat ctttcgcctc ttttaatacc ggttcccaac ctgtcgcgac
    25861 caccggagcg ataaagatcg catccacccc ttgtgcaacg aaggaacgta ccgctttaat
    25921 ctggttttcc tgcttttgct gaccatcggc aattttcagc gtaattccgc gtttttcggc
    25981 ctcacttttc gccacattgg tttctgcggc gcgccagccg gattccgatc cgacctgcga
    26041 aaatcctacg gttaatggag cggccaacgc catagacgac atggctgccg agactgcaga
    26101 gactacaagt aaacgtttcc acatagggtt gtcctcgtcg gggtagttat tattggttaa
    26161 aagatgttcg cgaaaaaact atagacaatc ggtgatgtaa cgaattgcgt tacatcacac
    26221 ttcaaaaaag taaataaaac gttaatcaca agtttgtaat cgctttcatc tcactatgaa
    26281 aaatgcggct acggttatgg attttcctgc tctgcatacc gtcttaaaac tggcgaaaaa
    26341 ggaaaatgaa gacgaaaaca agcgaagaca ttcggcgcga gttggctata atactcggca
    26401 cttgtttgcc acatattttt aaaggaaaca gacatgagct tactcaacgt ccctgcgggt
    26461 aaagatctgc cggaagacat ctacgtggtt attgagatcc cggctaacgc agatccgatc
    26521 aaatacgaaa tcgacaaaga gagcggcgca ctgttcgttg accgcttcat gtccaccgcg
    26581 atgttctatc cgtgcaacta cggttacatc aaccacaccc tgtctctgga cggtgacccg
    26641 gttgacgtac tggtcccgac tccgtacccg ctgcagccgg gttctgtgat ccgttgccgt
    26701 ccggttggcg ttctgaaaat gaccgacgaa gccggtgaag atgcgaaact gattgctgtt
    26761 ccgcacacca agctgagcaa agaatacgat cacattaaag acgttaacga tctgccagag
    26821 ctgctgaaag cgcagatcgc tcacttcttc gagcactaca aagacctcga aaaaggcaag
    26881 tgggtgaaag ttgaaggttg ggaaaacgca gaagccgcta aagctgaaat cgttgcctcc
    26941 ttcgagcgcg caaagaataa ataagttctt ctggcgcaat aaccctgaac accgggcttc
    27001 ggttagtaag ggttttttta tgcccgcgat aaataaacac accttattcc accaccgcct
    27061 gcaagcgtaa taacgcctct tccaccacct catccgcagc cagaccaata cgctttgcca
    27121 gccgggcgcg tagatccatc atccgcacct gattcaccag caccacgccg tgcacatcgc
    27181 cttcttcgca atgtaaagga acgctaaatc cggcataacg ggcaaaattt ccgccctgcg
    27241 taatgggggc caccagcgtc attcccagtt gattaaaggc ttgaacggag agcacaagcg
    27301 caggtcgacc agcaccttgc tgttcatggc cgcttgctgg atcaaagcca accagcacaa
    27361 tgtctccccg ttcaaattca cttttcttta ccatatttcg tcacccgcag gggtggattt
    27421 accccagaca tcctgctcgc taagttccgc ggcgttcatg tcacactgtg ccagcagttc
    27481 atcaagcgag tagcgcctgg agatgggtgt cagaatcagt tggttgttgc tcacctgcgc
    27541 ttccacgctc tgccccggct gtaagttaag ttctttcatt acgatattgg gaatgaccat
    27601 acctgcactg ttcccccatc tttttatggt aatacgcatc tgcacctcct taagttatac
    27661 aaagtataac tcacggtgct acaggaaacg ttttacccct gcacatttgt gaacgccagc
    27721 agagtttttt atgcgattgc agatatggaa agcggctcgg agagaaaagc aaaagatgga
    27781 aaagttacaa aaaaaaatcg cgagatacat cgcataaaac aacgccaccc gaaggtggcg
    27841 tgcttttatt attacttatc cctgtctaac cagtcgccgc tttcaattag ctttaatcca
    27901 tcgacgggtc gttgataaac gtacatccat gcactcccgt acggcgtctg aatcaactgg
    27961 cgcgcgtatt caccgcccct ggtgcgcaag gcatcaagtt cggccagcgt ggcgttgtca
    28021 atacgataaa cttcaccgtg tactgttccg ttccccggaa ctgcgcctgg atagtggccc
    28081 aggctataca actggtagtt atcgatactg aaatcgccca gtaactgggc attggtcatc
    28141 cagtgactgt tgccttgttt gtggcgtaaa ctgccgtaga caaatattcg cattgctaaa
    28201 actcgaactg atagagcaaa tccagtgcct ggtctacacc agacacggct tccagatata
    28261 gcttaggcat caggcgataa cgtaacgtga gtgttgctat agagtcaaat atacccacgc
    28321 cgtatttcac ttgcagacct ggcaatacat agccgctgac cactacctgg gaggagtcgc
    28381 ctactccctg ggtgtcgagc gctaaattgc ttacgccaaa cgtctcgccg attttaccca
    28441 caatctggcc actttgcgca acccccaggc caatcagcat tgaggtcatc gccgcactgt
    28501 cgctctgatc gctctccagc ccttgtccac gtagcaaata agacaatgca gcttgttgcg
    28561 acatagccgg gtcagagaag atctccgctt tcggttcgtc cgccagacca gtgacgcgaa
    28621 ctccggcgat tacgtcgtct tctgtagcat acgggttacg aatagcttca atattaagat
    28681 acggttgatc tggcggacca gagaacagta gctcaccctt acgcacaatc agatcctgac
    28741 cataggcatg gaagcgccct tcagggatgt tgatctgccc gttcaggccc agcccttgtt
    28801 tgtcctgaac gacgttaaga tcgcccgtca gccgcgcttt caggccaaag gcgtcaatgc
    28861 gcacattgtt gccaacgtgg acaatcaggt tactgttaat cggaatcgac gccgttttcg
    28921 gctcttccgg ttgcaggtta tcgttaagca tcaccacatc gctggagacg cctactgcgc
    28981 tttccggcag atcgtgcacc acgatgcgcg cccacgggac atccacgcga ccatcgaggg
    29041 taaacaggtt tggtgtagcc tcgaatacaa catctggcga tacatccatt cgtaccatcg
    29101 gcggcacggt gatccgcact ttactgcctt tcgccgttac tcgtgcccgc cagttttcaa
    29161 tttggctcca gtcggcatca ccgttcaggt agatttcacc ctgctgggtc cgtacggtac
    29221 cggcaagcgt cgagcgcata ccgttaaagt tgaccgcaag ctggctcggc tgcatatcaa
    29281 acggcataaa gttgccgtcg atatccacac ccgtaacctg aagctgacca aacaactgcg
    29341 ggctttgcac atcaccaccc agacgcaagt tggcactcac catccccgct gctttttccc
    29401 cacgggtaaa gatggggttt atcatcgcaa ggttgaagtt acggatattg acgttgccac
    29461 caagattacg gcggccttgc ggatcggtca cctgcacctg tccatcaaac tggccgttat
    29521 tggtcaggcg gatggtccag cccaattcgg cacggttgtt acgcaattcc gccgtcagat
    29581 tcagtgtctg aaacgccacc ggcagcgccg catcgttgac ggtttgcgtt acctgcacgt
    29641 tacgccccga aagggtgata ctgccctgcg gcagcccctc tttcgtggtg tcccaggcaa
    29701 catccgcttt acccgtgaag ataccgctgg cctgagtggt ttctggcata aacggtttca
    29761 gcatggcgag gtcgaagcgg ttgagattca ccaccgcacg cccttcggcc cccgcatcga
    29821 tagtttgcgg cacgcacagt tccgcattcg ggttaagcca gcagtgtggc ccgatgctga
    29881 ttttttgctc cttattgcgg taatccagcg caatatcgcg ggtcagcgac cacgggccga
    29941 ccggcgtctg gaagcgggta ttgctaagag ttcccttcca gcgttcttct ttgcgatcaa
    30001 aacttcctgc cagattaagc tgcccggaaa ccggctcgcc ctgaatccgc aactgtagct
    30061 cgtgctgctt ttcgctgcct ttggcattca gggtgacgag gttgatattt acatccggct
    30121 gcgaaatttg ctcaacgcgt acgtcgagtt tcccggcgat ctgatcggtg gatttaatgt
    30181 cgccttccac gcgaacctgc gtcacggaaa gttcctgcca gcgcaggccg cgcgcggtga
    30241 tatctgccag tagttgtggc gcttccaccg tgccgcgtac tttcaccagc cctttcgctg
    30301 taccgccaag ccccggcagc gcgttatcca gccccggcgc gttgatggtg gcatcaagat
    30361 tgagatcttt tacccccagc tcgcctttca cttcggcact gtttggcccc agttccagat
    30421 gaagccctgg gatcatccac tgcatataac tgttgccttt cagcgtgccg tcaacgttca
    30481 ctttgttctg tttgacgtta ccggtcagct tcagttctgg cacctccatc tgccaggtgc
    30541 cgccgtacag gctaccgcgg gttttaatca agccattgag tttcgacggc cactccggga
    30601 tctctttggc ggtgttaatg ccgttaagcg ttagctcacc gcgccaacta atggcctgct
    30661 gccagtcgag caacgccttg agttcagttt tcccttccag cgccgcgacg gtgagtttgt
    30721 cgagattgac ctgctgttca ttacctttgg catcaagggt aatggtggct ggcgggatct
    30781 ccagtccctt cactgccgta cgcatagaga gcgtgtaatc ggtcattttg ccggtaagtt
    30841 tcagtttcag atcatccgcc tgatactgct tctcaccggt gaacggccag taaagctgtt
    30901 tgctgttcac ttccacgttg agtggcaatc cggcttccgc cagtcgcgtc tgggcgcgta
    30961 aatccatatc caccggaccg gaaaggttaa cgccaatctc cagctgttcg cgcagcgcac
    31021 cgcccacttt cagcttcagt ttttctcctt tcaacggctc cacgtttagt gtgcagttga
    31081 gggtgatatc caccggccag ttgtctgaca gctgcgccgt gccgctggcg ttgacgatcc
    31141 cttgactgga atcgatatcc agggcgtcca gtttagtatt gccgtcaatg ctgctcactt
    31201 tcagcagcat ggtgcgcacg gtgatgtccg tgtcgcccgt cacgcgtaac tgctcgcctt
    31261 taaactcttc aatgttcagg ttaagcggca aatgcacgtc ggtcatttcc ggcaataccg
    31321 ggcgagaaaa gagatctttc agcgtttcgc cgagcggctt ttcctccggc tgcggatttt
    31381 caattttcgg ttcgaccact tcttcctgcg ccacttccgc cactttcggc agagcaatca
    31441 gcaggccttt cagcgacgtc ggtttcaggg tcagggtttt ctcctgccag ttcaggccgg
    31501 aggtgaagtc catcaccgac accgtggtgt catcaatctt gatgttgacg ttgtccagcg
    31561 ccacccgtgt cagggtgatg ggatacggcg tggagagatc cagcggaccg ctatcttcct
    31621 cttcttcaac ctgttcagaa ggaggcattt ttttactgtc gatgttgacc tgaatgtctt
    31681 tcagcgccag gtcattaata caaacgctac tgttccacag gcactcaaga ccgaccgcca
    31741 gatgcagatt acccgctttt accgccacgc ctggctgctc ataacgaacg tcagacaagg
    31801 tgagatcgcg ccagccgccg gtgaccttgc caatatccag tcctggcacc cagcgttccg
    31861 ccgctttaaa tacaagatgc aggccgctgg tggtgcccac cagaaacgcc accgatccca
    31921 gcaacagtaa gataacgata accacgccga ggctgatttt tttccataaa ctcataattc
    31981 tggccccaga ccgatgtaaa actgtaaccc gtgttcgtct ttatccgcga ccggtacggc
    32041 aaaatcgagt ttgattggcc cgaccggcga ttcccagcgc acgccgaccc cggtaccggt
    32101 tttaaagtcg ctgcggcgaa tatcgcttac cgcttcgcca ctatcgacaa acaccgcgcc
    32161 ccaccatttt ccggtcacgt tgtactggta ttccagcgat ccggttatca acttcgaggc
    32221 ccctttcagg tcaccgttgg cgtatttcgg ggcgatagat ttgtatttgt agccgcgaat
    32281 actgcgatcg cccccggcga agaaacgcag atccggcggt actttgtcga aatcaccggt
    32341 ttcaatccag cccagcgtgc cgcgtgtaac gaaacgatgg cgatcgtaca gtgtgcggat
    32401 ccagacgttc tgcgcctgga aaacggagaa atcgacatct gaaccccagg ccgtgttgga
    32461 gtagtcgata gagtagcgtt gcgagtcgcc ccaggttggc atcaggccac cacgagaacg
    32521 cgtgcggcta atcatcaccc caggataaaa cagcatcgtg gtgttggtaa tttcaccctg
    32581 ggtaaagtgg tcgaggctcc agcgcaggtt aatggcacgc tgccagccgc tggagagatc
    32641 ccagtagcga gaagccacca gcgtagtgga gtcagattcg gtatcgttca ggtcagtgcg
    32701 cttaaaaccg ccctgcacca aataatattg ttccagtgga ttcttcagca gcggcatttt
    32761 atagctgaag tcgagggtct gttccggcgc ggaaatactg gtactggtgg tcagactgtg
    32821 accataagag ttcatccacg gctttttcca cgtcgctttc acgcgcggtc ccacgtccgt
    32881 agagtaaccg accccggttt cgatggtgtt ttcagttcgc ggcgaaacca cgcccgtcaa
    32941 gggtaatact ttcgtttcgc gcgctttatc aaattgtgga gccaccacca ccgagttaaa
    33001 ccagccggta gcagaaagtc ggcggttcag ttccgccaga tctttcgatt cgtactcatc
    33061 gccctcttta aacggcacca gattttgcag gtattcatcg cggatttgtg atccttcaaa
    33121 ggtcacatgc ccaaagcggt aacgttcgcc actgttataa tcaatatccc agaaggcttt
    33181 atgcaggccg agcgcaatgc ccagctgcgc tttggtaaat tcgctatcga aataaccttt
    33241 acgcaacgca atgctggtta aggacttttt gaaattttca taatcgccct ggttcagcac
    33301 cgtgccaata gccgggcgag tatcgagcaa tttcaaatag tctttatcgg tccgcgcgcc
    33361 gccgcgcaat accacatcgg tgccgccaat taacaccggc acacctggcg tgactttggc
    33421 gatcaatacc tgccgccctt tctttggcgg tggacggaga tcaaattcaa tggtcggctg
    33481 gtaataaccc agcgctttca gaccttcgcg gatggcatca tcgacgcgtg cgcgaaagcg
    33541 acggtctggc gtcacttcat cactttcaat cgtagaaagc tgcgcacgaa cgttcttttc
    33601 cagctgtccc gataacccct cgacctgtag acggacgttc gcggcgacgg cagatccgct
    33661 taagcagagt aagcttacac agcataactg tcggatatag cgcacatttt ctcctgaata
    33721 tccttttttc ctgcccctgg aacgccgtta aacggcttaa caaaaatcca gtaatatgga
    33781 ttaaaaagca gactaaaccc caaatatttc ttatgtttta ctttagacct attcacggtg
    33841 gttattgtgt gcaaatacgc ctcttgttac aaccttaacc ccaatgaccg attttcggga
    33901 gagcgacacc atgagtttat ttgataaaaa acatctggtt tcccccgccg atgccctgcc
    33961 tggacgtaac accccgatgc ccgtagccac gctgcatgcg gtcaacggtc actcaatgac
    34021 caatgtacct gacggaatgg agattgccat ttttgcgatg ggttgtttct ggggagtgga
    34081 gcgtctgttc tggcagttac ccggcgttta cagcaccgcc gcaggctata ccggaggcta
    34141 tacgccaaat ccgacttatc gggaagtgtg ctccggtgat acgggtcatg ccgaagcggt
    34201 acgcattgtt tacgatcctt ccgtcatcag ctatgagcag ttgctacagg tattttggga
    34261 gaatcacgat cccgcccagg gaatgcgtca gggcaatgac cacggcacgc agtatcgttc
    34321 agcgatttat ccgctgaccc cagaacagga tgccgcagct cgcgccagtc tggaacgttt
    34381 tcaggcggcg atgcttgccg ccgatgacga tcgtcacatc accacggaaa tcgctaacgc
    34441 cacaccgttt tattatgccg aagatgacca ccagcaatat ctgcataaaa acccgtatgg
    34501 ttactgtgga attggcggaa ttggcgtctg tctgccaccg gaagcatagc gttacgggta
    34561 caaatgtaga ttgttgataa agtgctcttt gtctatgccg gatgcggcgt gaatgcctta
    34621 ccaggcctac aaaatcgtcc aaattcaata tattgcaggg actgcgtagg cctgatcggc
    34681 atagcgcatc aggcagtatt gcatttatca gcagtctgaa ttttaaccct ctggcgactt
    34741 tacagtacct tacgctatac tagccactga aaatgccggt tcactttctt cgaatcggct
    34801 ttcaatgtgt atttcacaca aattaatcaa cttccccttc cgaggatctg gcctgaaagg
    34861 tcggataaga tatgttaaac agtattttag tcatactctg cttgatcgct gtaagtgcgt
    34921 tcttctcgat gtctgagatc tcacttgccg cctcacgcaa aatcaaactt aaactgctgg
    34981 ctgatgaagg caatataaat gcccaacgcg ttctgaatat gcaggaaaat cccggcatgt
    35041 tctttacggt ggtccaaatc ggtctgaacg cagttgcgat tctcggcggt atcgtcggtg
    35101 atgcggcatt ttctccagct tttcacagcc tgttctcccg ctatatgtcg gcagaactct
    35161 ctgagcaact gagctttatt ctctctttct cgttagtgac tggcatgttt atcctgtttg
    35221 cggatttaac cccgaaacgc atcggtatga ttgcgccaga agcggtggct ttgcgtatca
    35281 tcaacccgat gcgcttctgc ctgtacgttt gcaccccgct ggtgtggttc ttcaacggcc
    35341 tggcgaacat gatcttccgt attttcaaac tgccaatggt acgtaaagat gacatcactt
    35401 ctgatgacat ctacgcggta gtggaagccg gtgcgctggc gggcgtgtta cgtaaacagg
    35461 aacacgagct gattgaaaac gtctttgagc tggaatcccg taccgttccg tcttcaatga
    35521 caccgcgtga aaacgtgatt tggtttgatc tccacgaaga tgagcaaagc ctgaagaata
    35581 aggtggcgga acatccgcac tctaagttcc tcgtctgtaa tgaagatatt gaccacatca
    35641 tcggttatgt cgattctaaa gacctgctga accgcgtgct ggctaaccaa agcctggcac
    35701 tgaacagcgg cgtacaaatt cgcaacacgc tgattgtgcc ggatacgtta accctttcag
    35761 aggcgttgga aagttttaaa accgcaggtg aagacttcgc ggtgatcatg aacgagtacg
    35821 cgctggtggt ggggatcatc accctcaacg acgtgatgac cacgctgatg ggcgatctgg
    35881 tcggtcaggg gctggaagag cagattgtcg cccgtgatga gaactcatgg ctgattgacg
    35941 gcggcacccc aattgacgac gtcatgcgcg tgctggatat tgacgagttc ccgcagtcgg
    36001 gcaactacga aaccatcgga ggctttatga tgtttatgct gcgtaagatc ccgaaacgca
    36061 ccgattcggt gaaattcgcc ggctacaaat ttgaagtggt ggatatcgat aactaccgca
    36121 tcgaccagtt gctggtgacc cggatcgaca gcaaggccac cgccctttcg ccaaaactgc
    36181 ctgacgctaa agataaagaa gaaagcgtcg cgtaaccccc agaaacatca acggctcctg
    36241 aatcaggagc cgtcttatta ctgcatagca ctttggttaa gccatctctg tttgcagacg
    36301 cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct tcacttttgg
    36361 gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt ataaccgtcc
    36421 acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt ggaaaatttt
    36481 cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc caactattat
    36541 gaggcgcaaa ttttaatgcc tagtgactat agactatccg ggcaatgttt ccaccgcgta
    36601 taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg aagcgcggtc
    36661 ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac tggcatcaca
    36721 cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag tggtcgtatc
    36781 acctgcatat cataagaagg aaacaccatg accctacgca agattctggc actcacctgc
    36841 ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg agtgccgccg
    36901 attggcatca ccgatcgtgg cgagttggtg cttgataaag atcagtttag ctacaaaacc
    36961 tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc tggtcgcacc
    37021 tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa gttaccgcac
    37081 gatcgttacc agaccaccac cattgttaac accgacgacg caattccggg ttccggcatg
    37141 tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca gtttattgtt
    37201 gatagcaatg gcgtcgcacg cggtgcctgg cagctggatg aaaaaagttc cgctgtagtg
    37261 gtgctggata aagacggtcg cgtgcaatgg gccaaagacg gggcgcttac tcaggaagag
    37321 gtgcagcaag tgatggacct gctgcataaa ttaattaata aatagacact ctgaacccag
    37381 gattcaggaa cgactcgcgc ggagtgtaat ccagcggttt accctgccag tcgtgaacgt
    37441 gcgctccggc agctgcggcc acagcatgtc cagcggcggt gtcccaaata ttcgttggtc
    37501 cgaagcgcgg gtacagctgc gcctgtcctt ccgccaccag gcagaatttc agcgaagagc
    37561 cgatggacgt ggtctgatgt tcgccaagct gttgcagata ctctttcagc tccgcatctg
    37621 catgggaacg gctgataacc accagcggcg ggcgcgcatc gcggacctga atctgcttgc
    37681 gcacaccgca ctcttctttc caggccttgc cttctgccgc gctgtacatt acgttcatta
    37741 ccggcgcata caccacacct aaaatcggtt tgccatggtc aatgagcgca atgttaacgg
    37801 tgaattcgcc attgcgttta ataaactctt tagtaccatc cagcgggtct accagccagt
    37861 aacgctgcca gtgctgacgg acttcccaac cgggaggatc ttcttcagaa aggaccggaa
    37921 tatccggtgt cagcgtacgt aaaccgtcca tgataacggt gtgtgcggca atatccgctg
    37981 ccgttaccgg agaattgtcc gctttgctga cgacgtccat cggtttcgtc ccgtcgtaga
    38041 cctgcataat ggcatcgcct gcattccgtg caagctggca tacttgatct aacatttctc
    38101 cacctcgtct ctgtgagcgg tgttaactta ttgttttact tataccctat cgttaatgaa
    38161 tgcgccaact gtgatagtgt catcattttc aaagcgtaaa attgtggcat tcttcactgt
    38221 tctataagta agacgtttat tctccctttt ctttcgtatt cccgatgata aaaggatgtc
    38281 cctgatgatt aagtttagcg caacgctcct ggccacgctg attgccgcca gtgtgaatgc
    38341 agcgacggtc gatctgcgta tcatggaaac cactgatctg catagcaaca tgatggattt
    38401 cgattattac aaagacaccg ccacggaaaa attcggactg gtacgtacgg caagcctgat
    38461 taacgatgcc cgcaatgaag tgaaaaacag cgtactggtc gataacggcg atttgattca
    38521 ggggagtccg ctggccgatt acatgtcggc gaaaggatta aaagcaggtg atgttcatcc
    38581 ggtttataag gcgctgaata cgctggatta tacggtcggt acactcggca atcatgaatt
    38641 taactacggc ctggattacc tgaaaaatgc gctggcggga gcgaaattcc cttatgtaaa
    38701 tgccaacgtc attgacgcca gaaccaaaca gccaatgttt acaccgtatt taattaaaga
    38761 taccgaagtg gtcgataaag acggaaaaaa acagacgctg aagattggct atattggcgt
    38821 cgtgccgccg caaatcatgg gctgggataa agctaattta tccggaaaag tgacggtgaa
    38881 tgatattacc gaaaccgtgc gcaaatacgt gcctgaaatg cgcgagaaag gtgccgatgt
    38941 cgttgtcgtt ctggcgcatt ccgggctgtc tgccgatccg tataaagtaa tggcggaaaa
    39001 ctcagtttat tacctcagtg aaattccggg cgttaacgcc attatgtttg gccatgctca
    39061 cgccgttttc ccgggtaaag attttgctga tatcgaaggg gctgatatca ccaaaggcac
    39121 gctgaatggt gttccggcgg taatgccggg catgtggggc gatcatcttg gtgtggtcga
    39181 cttacaactc agtaatgaca gcggtaaatg gcaggtgacg caggcgaaag cggaagcacg
    39241 accgatttac gacatcgcta ataaaaaatc cctcgcggcg gaagacagca agctggtaga
    39301 aacactcaaa gccgatcacg atgccacacg ccagttcgtc agcaagccaa tcggtaaatc
    39361 tgccgacaat atgtatagct atctggcact ggtgcaggac gatccgaccg tgcaggtggt
    39421 gaacaacgcg caaaaagcgt atgtcgaaca ttacattcag ggcgatccgg atctggcaaa
    39481 actgccggtg ctttcagctg ccgcaccgtt taaagttggt ggtcgcaaaa atgacccggc
    39541 aagctatgtg gaggtggaaa aaggccagtt gaccttccgt aatgccgccg atctttatct
    39601 ctaccccaat acactgattg tggtgaaagc cagcggtaaa gaggtgaaag agtggctgga
    39661 gtgctctgcc ggacagttta accagattga tcctaacagc acgaaaccac agtcactcat
    39721 taactgggat ggtttccgca cttataactt tgatgtgatt gatggtgtga attatcagat
    39781 tgatgttacc cagcccgccc gttatgacgg cgagtgccag atgattaatg ccaatgcgga
    39841 aaggattaag aacctgacct ttaacggcaa gccgattgat ccgaacgcca tgttcctcgt
    39901 tgccaccaat aactatcgcg cttacggcgg caaatttgcc ggtacgggcg acagccatat
    39961 cgcttttgct tcaccggatg agaaccgctc ggtgctggca gcgtggattg ctgatgagtc
    40021 gaaacgtgcg ggggaaattc acccggcggc agataacaac tggcgtttag caccgattgc
    40081 tggcgataag aaactggata tccgtttcga aacttccccg tcagataaag ccgcagcgtt
    40141 tattaaagag aaagggcaat atccgatgaa taaagtcgcg accgatgata tcgggtttgc
    40201 gatttatcag gtggatttga gtaagtaaaa cacttctttt tcggcctata aatcatcaac
    40261 cgcatccggc atttattggc ggatgcgatg ctggcgcata ttatccgccc tacaagccat
    40321 gcaccgtaga ccagataagc tcagcgcatc cggcagttat gccgcacgtt catcccgcac
    40381 cgccaacacc tcgggcaaat tcaactcaat ccagtccgcc agtgcagcaa ccttttcgct
    40441 cacctgctcg cccagcggcg tgaggctgta ttcaacatgc ggtggcacca ccggatacgc
    40501 gatacggtta agaaaaccat cctgttctaa cgcctgtaac gactgcgcaa gcatcttttc
    40561 gctcaccccg ccgattttgc gccgcaggtc gctaaagcga tgagtacctt cgcgtagcgc
    40621 caccagaatc aacacccccc aacggctggt gacgtgtttc aacacctcgc gcgacgggca
    40681 ctgttccgca aagagattac cctctttcag ttgttgcgac aggctaacct gactcatttc
    40741 atacttacct ttttgtacgt acttactaaa agtaagttta ggtgttagcg tatttaaact
    40801 caagacaaaa cgatggagac ttcccatgat cgctattact ggtgccactg gccaacttgg
    40861 tcactatgtt attgaatcct tgatgaaaac ggttcctgcc agccaaatag tggctatcgt
    40921 tcgtaatccg gcaaaagccc aggccctggc agcacaaggc attaccgtgc gtcaggctga
    40981 ctacggcgat gaagccgcac tgacatctgc acttcaggga gtggaaaaac tactgctgat
    41041 ctcttccagc gaagtgggcc agcgtgcccc gcagcatcgc aatgtgatta acgcggcgaa
    41101 ggcggcgggc gtgaaattta tcgcttatac cagtctgctt cacgcggata aatctccgct
    41161 cggcctcgcc gatgagcaca tcgagacgga gaaaatgctg gctgattctg gcatcgttta
    41221 caccctgctg cgcaacggct ggtacagcga aaactacctc gccagcgcac cggcagcact
    41281 ggagcacggc gtatttatcg gtgccgcggg cgatggcaaa attgcctcag caacgcgggc
    41341 agattatgcg gcagctgcgg cacgcgtgat tagcgaagcc ggtcacgaag gcaaggttta
    41401 cgaactggcg ggcgatagtg cctggacgtt gacacagtta gcggcagagc tgaccaaaca
    41461 gagcggcaaa caggttacct atcaaaatct gagcgaagcc gatttcgccg cggcactgaa
    41521 aagcgtcgga ctgcccgacg gactggcgga tatgctggcg gattctgacg ttggcgcatc
    41581 gaaaggcggt ctgtttgatg acagcaaaac gcttagcaaa ttgattggcc gcccaacgac
    41641 aacgttagcc gaaagcgtaa gccatctttt taatgttaat aactagttaa ttaaagtggc
    41701 atcctcccgc atcctctctg ataatgacgg gatgccggga gcaatcatgt ctgcttcctg
    41761 aacgtttctt ctgacagacc aatggatgcc agtaatgatt agcggcgtgc tgtacgccct
    41821 gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg ttgatcgtgc cggaataccc
    41881 ggcgatgttg cagtcgatgg ggcgttatct ggcgttaggg ttaattgcgc tacccattgc
    41941 ctggctggga cgcgtgcgtc tgcgtcagtt ggcgcgtcgc gactggctta ccgccttgat
    42001 gctcactatg atgggcaacc tcatttatta cttctgcctt gccagtgcca ttcaacgtac
    42061 tggcgcgcct gtttccacga tgattatcgg caccctgccg gtggtcattc ctgtctttgc
    42121 caatctgctt tatagccagc gcgacggcaa actcgcgtgg ggaaaactcg ccccggcact
    42181 gatttgtatt ggcatcggcc tcgcgagtgt gaatattgct gagttaaacc acggactccc
    42241 cgattttgac tgggcacgtt atacctctgg catcgtgcta gcgttagttt ccgtggtctg
    42301 ctgggcgtgg tatgccctgc gcaacgcccg ctggctgcgg gaaaatcccg acaaacatcc
    42361 gatgatgtgg gcaacagcgc aggcgctggt cacactgccg gtttcactta tcggctatct
    42421 cgtcgcttgt tactggctga atacgcaaac gccggacttc tccctaccct ttggcccccg
    42481 tccgctggtg tttattagtc tgatgattgc gatagccgtg ctttgctcat gggttggcgc
    42541 actctgctgg aacgtcgcca gccagcgatt accgacagtg attctcgggc cgctgattgt
    42601 tttcgaaacg ctggcaggtt tgctgtacac ctttttactc cgccagcaaa tgccgccgct
    42661 aatgacgctg agcggtatcg cgctgttagt gattggcgtg gtcatcgcgg tcagagcaaa
    42721 accagaaaaa cctttaactg aatctgtctc agaaagttga cacgctggca gtgagttaaa
    42781 taagcctctg ctacgtaagg gttatagctt ttgccttaaa gatgcattta aaatacatct
    42841 tatcttatta agaatgaggt atcagctatg gcttatcgcg accaaccttt aggtgaactg
    42901 gcgctctcta ttcctcgcgc ttcagctctg tttcgtaaat atgatatgga ttactgctgt
    42961 ggcggtaagc agacgctggc gcgcgcggcg gcacgtaaag aactggatgt tgaggtcatt
    43021 gaagctgaac tggcaaagct cgctgaacaa ccgattgaga aagactggcg tagcgccccg
    43081 ctggcagaaa tcattgacca tatcatcgtg cgctaccacg atcgtcaccg cgagcagttg
    43141 ccggagctga tcctgcaagc gactaaagtc gagcgcgttc acgccgacaa accgagcgtg
    43201 ccaaaagggc tgacaaaata cctgactatg ctgcatgaag agctttccag ccacatgatg
    43261 aaagaagagc agatcctctt cccgatgatc aaacaaggca tgggcagcca ggcaatgggg
    43321 ccaatcagcg taatggaaag cgagcacgat gaagcgggtg aactgctgga agtaatcaaa
    43381 cacactacca ataacgtcac accgccgccg gaagcctgca ccacctggaa agcgatgtat
    43441 aacggcatta atgaactgat tgatgacctg atggatcaca tcagcctgga aaacaatgta
    43501 ctgttcccac gcgcgctggc gggtgagtga tctaaaagct ggatggcatt gcgccatcca
    43561 gcatgataat gcggttattt ccgcagttca gcagcccgct tcttaccaat aaacagccag
    43621 cccaacccca gcgcgataaa ccacagcgga gtaaccagca gcgcctggcg agtgtcatct
    43681 tccagtgtca gcaacacgac cacgaacaca aagaacgcca tacataccca gcacatcagc
    43741 ttgccgagcg gcatcttgta gatcgacttc tcatgcagat gaggacgctg tttgcggtac
    43801 accaggtacg agcaaaggat aattgtccag acgaacataa acagaatcgc ggaaacggtt
    43861 gtaatcatcg tgaacgcgcc aatcacgcta ggattcacat acaacatcac cacgccgccg
    43921 agcagacaga tacacgagaa ggtcaaccct ttcgcgggta ctgcgcgctt agaaagttta
    43981 gcgaacgctt tcggcgcaac accttcctgc gccagaccaa acagcatacg gctggtagag
    44041 aagacaccgc tgttagcgga agacgccgca gaggtcagca ccacaaagtt gatcacgctg
    44101 gcagccgcag gcaaacctac caacacaaac agctcaacga acgggctttt ctccgggact
    44161 accgaactcc acggcgtcac ggacataatc acaatcagcg cgaagacgta gaacataatg
    44221 atacggatcg gaatggagtt aatcgcgcgt ggcagtgatt tctccggatc tttggtttcc
    44281 gcagctgtag tacctaccag ctcaatcccc acgaaagcga aaaccgctat ctggaatccg
    44341 gcaaagaagc cacttaaacc tttcgggaac cagccgccgt cattccacaa atgcgcgaat
    44401 gacgcttcca caccggtcgg tgactgaaag tgcatcgcca ccatgaccag gccgacgaca
    44461 atcagcgaca cgatggcgac gattttgatc atcgcaaacc agaactccat ctcaccgaac
    44521 attttcacgg tggcaaggtt gagcgtcagc agcagcacta tcaccgccag cgaggcgacc
    44581 cagtcggaga gatcggggaa ccagaactga gcataagccg tgatcgccac cacgtctgcc
    44641 ataccggtta caacccagca gaaccagtag gtccagccgg tgaaatatcc tgcccacggc
    44701 ccgagtaaat cggaagcgaa gtcactaaaa gatttgtatt ccagattcga aagcagcaat
    44761 tcccccattg cccgcatcac gaaaaagagc ataaaaccaa tgatcatata aacgaaaatg
    44821 atcgacggcc cggcaaggct aatcgttttg ccggacccca taaacaaccc cgtaccaatg
    44881 gcaccgccaa tggcaataag ctgaatatgt cggtttgtga gattgcgccg tagcgactgt
    44941 tcagccggag cctgatcatc ggcaacgact tttacctgat ctaccatgtt tttttcttcc
    45001 tgtacctgtc tgtgttgttc aggctctacg gcctttagtc agtctatgat acgacgaatc
    45061 tgtatcggga ctcatcgata ttaggtaaca atacgcggga tgaatactaa gatttagatc
    45121 taatgttaat tttatgttta aagtgagtgt catatcacgg tgataacgcg aaacagctca
    45181 caaaaataca cgcttacggg gtctttgcaa cacaaaatag cgccgtttgg caaactgaag
    45241 ggtttattgc tgaatgcctg ctcccctctg gttcgagggg aacagaatgc gctgcttaga
    45301 gaatttccag cagttcgact tcaaacacca gggtactgaa cggagggatg gatgcgcctg
    45361 cgccgcgctc gccatatgcc agttcctgcg ggatagtcag ttcccattta gaacctaccg
    45421 gcatcagagt cagtgcttca atccagccag ggatcacgcc attaaccgga aattcagcgg
    45481 gttcaccacg agcaacggag ctgtcaaaca cggtgccgtc gatcagttta ccggtgtaat
    45541 gaacacgaac gcggtcggta cgtgccggaa ttgcgccttc accctggttg atcacgcgga
    45601 attgcaggcc agattcggtg ctattcacac cttctttttt ggcgttttct tccaggtatt
    45661 tcacaccttc agcagccatc gcctggaaac gctgacgacg aacggcatcg gcgcgctcgt
    45721 ggatttcacg cagcgcgcga tgcaccacat caaccggaac agccggatgt ttgccttcca
    45781 gcgcatcggc aatacctgca accagtgctt ctggcagcag cccttccagg ccagattcac
    45841 tcagttgttg ccctacctgc aaaccaatgc cgtagcttgc ttgcgcttcg atggtgtcaa
    45901 aagttggggt ggtcatcgta tttcctttct tagggtataa aagtagcggg cagcatatca
    45961 gccacacttc tttgggtaaa actttgtcgc aggaatgatg acaaatcccg gggaaaaaga
    46021 aacaatagca atatcccgta tgaatataat aaaggcgggt ttaccacgga catcatagcg
    46081 ttagcttatt cataggctat gattgaggaa caagacgcgg agcaggagga aaccatgccc
    46141 gggcgctttg aactaaaacc aaccctggag aaagtctggc acgcgccgga caattttcgc
    46201 ttcatggacc cgctgccgcc gatgcatcgt cgggggatca ttattgccgc cattgtattg
    46261 gtggtcggat ttctgcttcc atctgatgat acgcccaacg cgccggtggt gacgcgtgaa
    46321 gcgcagttag atattcagtc gcaatcacaa ccgccgacag aagaacagct gcgggcacaa
    46381 ttagtcacac cacaaaatga tcctgatcag gtggctccgg tcgctccaga acctatacag
    46441 gaaggccagc cagaagagca gccgcaaact acccaaacgc aaccattcca gccagatagc
    46501 ggaattgata atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat ggcgcagctg
    46561 ttccgcgacc acggactgcc cgctaccgat gtctatgcaa tggcgcaggt agaaggcgcg
    46621 ggtaagccgc tgagtaattt gcaaaacggg caaatggtga aaatccgcca gaacgccagc
    46681 ggcgtggtga cgggcttaac cattgatacg gggaataatc aacaggtcct gtttacccgc
    46741 cagccggatg gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa agccagctaa
    46801 ggattgtctc tttgatgacc ttacgtctgg cttccagtgc ctggggggaa accatactga
    46861 tatgataaac caggccaagc gtatgctggt tgatcaaata atatccgcca agcgcagcaa
    46921 tattaatatt cacctgcaat gggtcgatat ctggcttaaa aatattgtgt ttcttccctt
    46981 catccaataa ggattccatt aactgcaagt gcgcatggtt aatttctaag agacgctgtg
    47041 attttgcata atgcacacct ttactttgat tttcactatg cacaat
//
LOCUS       DAZFWP020000015        45715 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFWP020000015 DAZFWP020000000
VERSION     DAZFWP020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 45715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.015"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(251..1255)
                     /gene="trpS"
                     /locus_tag="ACHK0Z_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     CDS             complement(251..1255)
                     /gene="trpS"
                     /locus_tag="ACHK0Z_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU4500878.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1248..2006)
                     /gene="gph"
                     /locus_tag="ACHK0Z_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             complement(1248..2006)
                     /gene="gph"
                     /locus_tag="ACHK0Z_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU4500879.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1999..2676)
                     /gene="rpe"
                     /locus_tag="ACHK0Z_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             complement(1999..2676)
                     /gene="rpe"
                     /locus_tag="ACHK0Z_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU4500880.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2694..3530)
                     /gene="dam"
                     /locus_tag="ACHK0Z_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     CDS             complement(2694..3530)
                     /gene="dam"
                     /locus_tag="ACHK0Z_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4500881.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3637..4923)
                     /gene="damX"
                     /locus_tag="ACHK0Z_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     CDS             complement(3637..4923)
                     /gene="damX"
                     /locus_tag="ACHK0Z_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU4500882.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPATKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYGNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(5015..6103)
                     /gene="aroB"
                     /locus_tag="ACHK0Z_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     CDS             complement(5015..6103)
                     /gene="aroB"
                     /locus_tag="ACHK0Z_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU4500883.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(6160..6681)
                     /gene="aroK"
                     /locus_tag="ACHK0Z_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             complement(6160..6681)
                     /gene="aroK"
                     /locus_tag="ACHK0Z_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU4500884.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7082..8320)
                     /gene="hofQ"
                     /locus_tag="ACHK0Z_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             complement(7082..8320)
                     /gene="hofQ"
                     /locus_tag="ACHK0Z_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU4500885.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8232..8636)
                     /gene="hofP"
                     /locus_tag="ACHK0Z_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             complement(8232..8636)
                     /gene="hofP"
                     /locus_tag="ACHK0Z_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU4500886.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(8626..9066)
                     /gene="hofO"
                     /locus_tag="ACHK0Z_RS04105"
                     /old_locus_tag="pgaptmp_000821"
     CDS             complement(8626..9066)
                     /gene="hofO"
                     /locus_tag="ACHK0Z_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU4500887.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(9050..9589)
                     /gene="hofN"
                     /locus_tag="ACHK0Z_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             complement(9050..9589)
                     /gene="hofN"
                     /locus_tag="ACHK0Z_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU4500888.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9589..10368)
                     /gene="hofM"
                     /locus_tag="ACHK0Z_RS04115"
                     /old_locus_tag="pgaptmp_000823"
     CDS             complement(9589..10368)
                     /gene="hofM"
                     /locus_tag="ACHK0Z_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU4500889.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            10488..13040
                     /gene="mrcA"
                     /locus_tag="ACHK0Z_RS04120"
                     /old_locus_tag="pgaptmp_000824"
     CDS             10488..13040
                     /gene="mrcA"
                     /locus_tag="ACHK0Z_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU4500890.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(13205..13765)
                     /gene="nudE"
                     /locus_tag="ACHK0Z_RS04125"
                     /old_locus_tag="pgaptmp_000825"
     CDS             complement(13205..13765)
                     /gene="nudE"
                     /locus_tag="ACHK0Z_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU4500891.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            14085..16220
                     /gene="igaA"
                     /locus_tag="ACHK0Z_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             14085..16220
                     /gene="igaA"
                     /locus_tag="ACHK0Z_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU4500892.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            16285..16953
                     /gene="yrfG"
                     /locus_tag="ACHK0Z_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             16285..16953
                     /gene="yrfG"
                     /locus_tag="ACHK0Z_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU4500893.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16964..17365
                     /gene="hslR"
                     /locus_tag="ACHK0Z_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             16964..17365
                     /gene="hslR"
                     /locus_tag="ACHK0Z_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU4500894.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17390..18268
                     /gene="hslO"
                     /locus_tag="ACHK0Z_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             17390..18268
                     /gene="hslO"
                     /locus_tag="ACHK0Z_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU4500895.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(18403..20127)
                     /gene="yhgE"
                     /locus_tag="ACHK0Z_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             complement(18403..20127)
                     /gene="yhgE"
                     /locus_tag="ACHK0Z_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU4500896.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            20505..22127
                     /gene="pckA"
                     /locus_tag="ACHK0Z_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             20505..22127
                     /gene="pckA"
                     /locus_tag="ACHK0Z_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU4500897.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(22203..23555)
                     /gene="envZ"
                     /locus_tag="ACHK0Z_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             complement(22203..23555)
                     /gene="envZ"
                     /locus_tag="ACHK0Z_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU4500898.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(23552..24271)
                     /gene="ompR"
                     /locus_tag="ACHK0Z_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             complement(23552..24271)
                     /gene="ompR"
                     /locus_tag="ACHK0Z_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU4500899.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            24499..24975
                     /gene="greB"
                     /locus_tag="ACHK0Z_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     CDS             24499..24975
                     /gene="greB"
                     /locus_tag="ACHK0Z_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU4500900.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            25072..27393
                     /gene="yhgF"
                     /locus_tag="ACHK0Z_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             25072..27393
                     /gene="yhgF"
                     /locus_tag="ACHK0Z_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU4500901.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            27831..28058
                     /gene="feoA"
                     /locus_tag="ACHK0Z_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             27831..28058
                     /gene="feoA"
                     /locus_tag="ACHK0Z_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU4500902.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            28075..30396
                     /gene="feoB"
                     /locus_tag="ACHK0Z_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             28075..30396
                     /gene="feoB"
                     /locus_tag="ACHK0Z_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU4500903.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            30396..30632
                     /gene="feoC"
                     /locus_tag="ACHK0Z_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             30396..30632
                     /gene="feoC"
                     /locus_tag="ACHK0Z_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU4500904.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            30835..31713
                     /gene="rpnA"
                     /locus_tag="ACHK0Z_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             30835..31713
                     /gene="rpnA"
                     /locus_tag="ACHK0Z_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU4500905.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGQVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(31742..32512)
                     /gene="bioH"
                     /locus_tag="ACHK0Z_RS04200"
                     /old_locus_tag="pgaptmp_000840"
     CDS             complement(31742..32512)
                     /gene="bioH"
                     /locus_tag="ACHK0Z_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU4500906.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            32550..33233
                     /gene="gntX"
                     /locus_tag="ACHK0Z_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             32550..33233
                     /gene="gntX"
                     /locus_tag="ACHK0Z_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU4500907.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSKAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            33292..33867
                     /gene="nfuA"
                     /locus_tag="ACHK0Z_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             33292..33867
                     /gene="nfuA"
                     /locus_tag="ACHK0Z_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU4500908.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            34228..35544
                     /gene="gntT"
                     /locus_tag="ACHK0Z_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             34228..35544
                     /gene="gntT"
                     /locus_tag="ACHK0Z_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4500909.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(35589..37673)
                     /gene="malQ"
                     /locus_tag="ACHK0Z_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             complement(35589..37673)
                     /gene="malQ"
                     /locus_tag="ACHK0Z_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU4500910.1"
                     /translation="MESKRLDNAALAAGISPNYINAYGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDHE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(37683..40076)
                     /gene="malP"
                     /locus_tag="ACHK0Z_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             complement(37683..40076)
                     /gene="malP"
                     /locus_tag="ACHK0Z_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU4500911.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            40688..43393
                     /gene="malT"
                     /locus_tag="ACHK0Z_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             40688..43393
                     /gene="malT"
                     /locus_tag="ACHK0Z_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU4500912.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(43436..44452)
                     /gene="rtcA"
                     /locus_tag="ACHK0Z_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             complement(43436..44452)
                     /gene="rtcA"
                     /locus_tag="ACHK0Z_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU4500913.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGIVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(44456..45682)
                     /gene="rtcB"
                     /locus_tag="ACHK0Z_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             complement(44456..45682)
                     /gene="rtcB"
                     /locus_tag="ACHK0Z_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU4500914.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        10836 a        12499 c        11590 g        10790 t
ORIGIN      
        1 tgaatgttta tatcttcatt aaaatgaaga taagtggcaa ctgtgagatg tggaaacggc
       61 gaggcgcttc acattttttc ggattattgc aaatgaggat aaaaaaaccg ggtttccccg
      121 gtttcagagt gatgacgaag tgatttattc gacaatgcga gcaaagggta attcccactg
      181 gcgcttgcct gctcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgag
      241 cgttttgata ttacggcttc gccacaaaac caatcgcttc gtacaccgct ttcagcgtac
      301 gggaagcgtg tacactggct ttttctgcgc cgtctttcat cacctgttgc aggaaggctt
      361 catcgttgcg gaaacggtga tagcgttcct gcaattcagt cagcataccg gaaacggcat
      421 cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt tccagttctg
      481 gaatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg cccgctttgt
      541 tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgcg cgtttgattt
      601 tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg ttatcgtcag
      661 acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca gatttcggaa
      721 taaacggctc cggcacctta aagatctcgc catacagcgc gttgaaacgc tgggcgatat
      781 cgcgactcag ttccaggtgc tgtttctggt cttcacccac cggtaccaga ttagtttgat
      841 acagcaggat gtccgcagcc atcagcaccg gatagtcaaa cagaccagcg ttgatgttct
      901 cggcataacg cgcagattta tctttaaact gggtcatgcg gctcagttcg ccgaagtagg
      961 tatagcagtt cagtgcccag cctaactgcg catgttctgg tacgtgggac tgaacaaaaa
     1021 tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta tccagcgtcg
     1081 ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca acgatacagt
     1141 aaatgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc atgtagttac
     1201 caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta gtcatttttc
     1261 gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt tatagactga
     1321 taaattacat caggctggct gagatcgata gcctcgccgt agttatatcc gtaggttaag
     1381 ccaactgatg ggcaacctgc cgcttttgcc gcctgaatat cattgcgtga gtcgccgaca
     1441 aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag cgggtccgga
     1501 tgcggttttt tgttttgcac atcatcgccg ccgataacca cgctaaagta tttggcaata
     1561 tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac caggcctagc
     1621 ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc
     1681 ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat acgtacctgt
     1741 tcttctgccg gaatgtcgtc atcaacgggc ggtttaccca ttgttttacg ctgagtcgca
     1801 cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc gttaccaatc
     1861 caggtaataa cgcgttcttc acctgcgacg ggcaactcca gcgcatacag cgccatatct
     1921 accgcagcag caagaccggg agcactgtcg accagcgtac catcaagatc aaaagcgacg
     1981 ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc gcatttcatc
     2041 aatgactttt ttgtagtctg gctggtcgaa gattgccgaa ccggcgacga acatatccgc
     2101 gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcta cttccagacg
     2161 aatgtcaaag ccagactcgt cgatacgacg acgtacttcg cgcagtttat ccagtgtttg
     2221 aggaatgaac gactgaccgc cgaaaccagg attgacggac atcagcagga tcacatccag
     2281 cttatccatc acgtaatcca gatagctcag cggtgtcgcc gggttaaata ccagtcccgc
     2341 tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg aggcttctgg
     2401 atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc gatcgacagg
     2461 tttcaccatc aggtgtacgt cgataggggc ggtaatgcca tagttacgca aggatttcag
     2521 caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt caaaatgcac
     2581 gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg caaaatcagc
     2641 cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga gaattatttt
     2701 ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac ctttttacgt
     2761 gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt tgcgcgctga
     2821 taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat atggcgctca
     2881 accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg
     2941 tgatacgccg taaagttggc ggtcgcagac agcggtgcat aaggcggatc gcaatagacg
     3001 acggatgcat catctgcgcg cgccatgcta tcggcgtaag actcacaata gaaaaaggca
     3061 ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg ttttttgtag
     3121 cggccaaacg gcacattaaa ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg
     3181 tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct tttgttgaac
     3241 tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa cagctcgcgt
     3301 gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact gatcaggtcg
     3361 ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac cgacccggca
     3421 cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat atcatcaagc
     3481 aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat gctgactaac
     3541 taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct tcaggcaact
     3601 cccgcaacga taaaccattg cgggagataa ccttgattac ttcagatcgg cctgtacctg
     3661 acgcagcggt ttcgcccacg ggtttttggc ctggacatct gctggcaatg tagataccgc
     3721 ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc acggctgacc
     3781 attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg cccaaccgtt
     3841 caggttgccg tagttagagg aactgctcag ctgcagagtg taatggctgg acggtgccga
     3901 tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg
     3961 cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttgttg ctggcgtaga
     4021 agtcgcagcc ggtgccttcg tgctcgccac aggggcagct ggttcagtac gcttcggcgt
     4081 ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc ggttttttcg gttcaatcac
     4141 tgcctgctgg cgagctggac gcgtagtggc cggacgttca gcggtttgcg ttttcgccgt
     4201 ttcacgggat gcattgccat tgcgaacagg cgcgacagtc gcgggttcgg tcggcaatgt
     4261 ggaattgacc gccacattgt tcaactgttg ctgattttgt ggttgggtca gcgcattgtt
     4321 caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccgccgggg tttgcccttg
     4381 agtcggcgta gaagagatcg gcggcagaga aacatcctgc tgagtatttt ccgcagacgt
     4441 ggttcccggc gctggctgca cgccattggc ctgatcggtc gcattgccag caagatcaat
     4501 actcttctcg ccagacgcgg tttgatcgct ggaagaggtc gagggggctt ttagcgcaga
     4561 accgataccg atgatcaaca gcagtagaac cagaatgccg acgcccatca tcatatactg
     4621 acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc
     4681 ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg gttcctcatt
     4741 gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt caatatcatc
     4801 aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag attgacgaga
     4861 acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt tgaattcatc
     4921 catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct gaagctaata
     4981 gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa tcggcaatgg
     5041 cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca attgccaacg
     5101 gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata tgcggtaaat
     5161 acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc gtgagcaggg
     5221 ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac gtccgcgccg
     5281 ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac cccatttcag
     5341 cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc
     5401 gctcgtcggc ggcgacaact tctgccttca gttcacaaca acggcgaata cagtacgcca
     5461 ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag ttaaaaaacg
     5521 caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct aactcacgcg
     5581 ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggctgg tagaacgcgc
     5641 caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg gaggaatcga
     5701 cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac accgcgctga taactcgccg
     5761 ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc gtagtatcgc
     5821 gaccatgcgg cttttgtaac aacgccgtaa agacggtatc gagtacagcc aggcttttat
     5881 actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt tcaagtacgc
     5941 cgcggacctt atcgagatac agaggagcca gggtttcgtt ggtgaccaac atgacctgct
     6001 cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat gcgatggtaa
     6061 ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga catccacctt
     6121 aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt ccagcatgtg
     6181 aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc
     6241 aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt cacgcggcgg
     6301 tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc
     6361 gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac gcgtttcacg
     6421 ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg tcaactcatt
     6481 gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc
     6541 cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag
     6601 ttgttgagct aactggcgcc caatagtgct ttttccggca cccataggcc caaccagaaa
     6661 gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta atgataaacc
     6721 cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata ttgcgagagt
     6781 cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat cacccatccc
     6841 cctgcatatc tggtcgctgc aagcgcgctg ccttgctacc accgctctgg cgataaatca
     6901 ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa atcatcatca
     6961 tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta tgctaaatac
     7021 ctgcgcgcgt caaatagatg aaacacgttc agcataaaaa cattaccgct tttacggctg
     7081 tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt cgttcatctt
     7141 cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc aacggtacgc
     7201 tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc
     7261 ttttgacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact tcgccatcgg
     7321 cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc cggatgcgac
     7381 ctttttgtaa caccgtgggc gtgacctcca tccccaggac ggcctcttta aattccaccg
     7441 acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctcccc tgtttaatgc
     7501 tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc agctgctgtt
     7561 tttgttcgag cgcagaaagc tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa
     7621 aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc
     7681 caacgccacc agcgtgttgc gcatcggcca gcgtccattt cacgcctaac tcacgcaaac
     7741 ttttttcatt aatggtgaca atatgcgccg acagctcaac ctgcccgacc ggcagatcca
     7801 tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgt agcaaaaggc
     7861 gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc tcccccgctt
     7921 tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc agcggcagat
     7981 ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat attgttattc tgccaggcaa
     8041 tggaatgcac tgagagaatg ttgtcttcct gccgcgttat cagtccggcg cttttcacta
     8101 cagtttgtag tgcctgcttc cagggaacat ctgttagatg taacgacacc gtaccgctga
     8161 cgtctggcga cacgaccagg ttcaacttct cctgttcagc cagcgcctgc aacacctgag
     8221 ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg
     8281 gtatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc ttgccgttgc
     8341 cacaaccatt gtggcggttc gcagtttgtc ccggttccca gcgttagtgc gtctggcgtc
     8401 agctgtaaaa ttgtccagcc gttttccagc acatcgtttt gctgcacccg tcgccatttc
     8461 ttttgcccgt cttttattac accgatgatg cgctcgcctc gccctaccat cccctgatag
     8521 cgccactggc taagttcgct aatccggcat agatcttccg gcggtttaaa agggtcacgc
     8581 ataccggtta aaaggcacaa tgcaatacct gccaacaacc agcgtttaac cctcatgcgg
     8641 cgtctccagt tgtagcgtga acaaaagatc atcaccttcc acgcttaacg aaaaacggct
     8701 cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt cccacagcgt
     8761 tttcaacgcc aactcacctc cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga
     8821 taactgaaaa tccagaggcg aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc
     8881 taccaggcga tacagtgcgg cccactgctg gtgatgactt gcccgtagtc gaattaatgc
     8941 gtcgcgctct tcatggtgtg tcgatgatag aaaaatgagc gtaactaaca tcagcaacca
     9001 gaatgcccag caaagctggc ggaggcgggg tgatgtggcg aaccaccagt caaagaacat
     9061 gttcatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc tgcgcatcct
     9121 gctgcgtggc cccccgctga ttgagatgaa aagaagcatc ctggcgaagt gacgtttcta
     9181 gtgcgtttaa cgcggtaatg cttgttgtaa gccccttgat ctccagcgtt ccctgctgcc
     9241 agcttatcgt tgtcagccag gcgtgttcag gtaaaagagc cgccagtgct tccagcgcag
     9301 attgccagtc gcgggtaaat tgtcgctggc gctgcctttg caaacgctgc tcacgaattt
     9361 gttggcgctc cagcaaacgt ggtttcgtta tctgtaagct gtgggcgagt tgttgttccc
     9421 cctgaagcaa aacggcgtct acgcgagctt cggcgctgct tgtcagacgc agtattagcg
     9481 ttatcccgac ggccagcagc agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag
     9541 cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc agtacgcctt
     9601 cccaagcgcc agccccagcg cgatggcaaa gtctccacct gacggcggta gcggcggctg
     9661 acgaacagaa acggcctccc agggatcaaa tcccccttcg ccacatatcg cgacgctttc
     9721 gggatcaacg gataacgctg ccgccagctc cttcgcgcta gtcatcccca ccgccagttt
     9781 gcgcccccag cgatagcgtg tcgcccacag ccactgttcg ttatcacgcc aggccagaca
     9841 ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat ccggggtgat
     9901 cgcactaaca tgaacacgca acctttctgc cagagtaagc agcgttgcca gctctttgct
     9961 ttgcgcggca gtcacgttat aagcggggct gagtgagtct tcgctataat cgaagcgcag
    10021 ggagtccgga tccatatcca gctcgcgggc catcgtccct gacagccagg ccgtttgctc
    10081 ccgctcacca agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg
    10141 aaacgccaac ataatgtgat gacgctgcgg cagttcgcga ctccaaggta acaacgtttt
    10201 agccagctgc tgcgcatcaa caatccgccc atctttgata atgtcgttct ccagcggcaa
    10261 ccgccaccag cgttgcaaaa agcattcttt tgcgccccgt acgatcgcaa ccgctaccgc
    10321 ttcttgctgt tgtaaatgca aaccaatttg ccagatctta aatgccattg tgatgatctc
    10381 cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt atactaccgc
    10441 gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg aagttcgtaa
    10501 agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg atttatggcc
    10561 tataccgcta catcgagcca caactgccgg atgtggcgac attaaaagat gttcgcctgc
    10621 aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac ggtgagaaac
    10681 gtcgtattcc ggttacgttg gatcaaatcc caccggagat ggtgaaagcc tttatcgcga
    10741 cggaagacag ccgcttctac gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg
    10801 caagtgtggc gctgttctcc ggccacgcgt cacaaggggc gagtaccatt acccagcagc
    10861 tggcgagaaa tttcttcctc agtccagaac gcacgctgat gcgtaagatt aaggaagtct
    10921 tcctcgcgat tcgcattgaa cagttgctga cgaaagacga gatcctcgag ctttatctga
    10981 acaagattta cctcggttac cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg
    11041 gaaaaacggt cgaccaactg acgctgaacg aaatggcggt gatagccggg ctgccgaaag
    11101 cgccttccac cttcaacccg ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg
    11161 tactgtcgcg gatgctggat gaagggtata tcacccaaca acagttcgat cagacacgca
    11221 ctgaggcgat taacgctaac tatcacgcgc cggagattgc tttctctgcg ccgtacctga
    11281 gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat gaagacggtt
    11341 atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcacagcag gcggtacgta
    11401 ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga
    11461 aagtgggcga gtcggcgtgg gataacaaca agattaccga tacgctgaag gcgctgccaa
    11521 cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcagcaa gcgacggcga
    11581 tgctggcgga cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt
    11641 accgttcgga tactcagcaa ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg
    11701 gtcagcaaat ctgggttcgt caggttggcg atgcatggtg gctggcacaa gtgccggaag
    11761 tgaactcggc gctggtgtcg atcaatccgc aaaacggtgc cgttatggcg ctggtcggtg
    11821 gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt caggtgggtt
    11881 ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctgacg ctggcaagta
    11941 tgttgaacga tgtgccaatt tctcgctggg atgcaggtgc cggttctgac tggcagccga
    12001 agaactcacc accgcagtat gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga
    12061 aaaacgtggt gatggtacgc gcaatgcggg cgatgggcgt cgactacgct gcagaatatc
    12121 tgcaacgctt cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt
    12181 cagcgtcctt caccccaatg caggtggcgc gcggctacgc ggtcatggcg aacggtggct
    12241 tcctggtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg
    12301 aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc ggtgatttac ggtgatacgc
    12361 agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc cgcgagcagc
    12421 agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga
    12481 ctggcgcgca ggagtacgca ccgcacgtca tcaacactcc gctggcattc ctgattaaga
    12541 gtgctttgaa caccaatatc tttggtgaac caggctggca gggtaccggc tggcgtgcag
    12601 gtcgtgattt gcagcgtcgc gatatcggcg ggaaaaccgg taccactaac agttcgaaag
    12661 atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg
    12721 atcaccgtcg taatctcggt catacaacgg cttccggagc gattaaagat cagatctcag
    12781 gttacgaagg cggtgccaag agtgcccagc ctgcatggga cgcttatatg aaagccgttc
    12841 ttgaaggtgt gccggagcag ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg
    12901 atcgcagcac cgggcagtta gctaatggtg gcaacagccg cgaagagtat ttcatcgaag
    12961 gtacgcagcc gacacaacag gcggtgcacg aggtgggaac gaccattatc gataatggtg
    13021 aggcacagga attgttctga ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa
    13081 gtgcatttgt ttatgccgga tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa
    13141 atcaatatat tgcaggaaac ccataagcct gataagcgtt gtgcatcagg caaacttcac
    13201 gcatttacac tcgcccctgc cctttcaacc attcgcgcac gaggaacagc gcgctgacat
    13261 tacgcgcttc gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc
    13321 gcacctgtgg tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg
    13381 cgaccacgat attcattttg ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa
    13441 aagtcagatc gttcgcacca aatccaacct cttcttttag ctcgcggtta gcggcttcgt
    13501 agacgctttc acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc
    13561 ccactgcgta ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg
    13621 cttcccggtt ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca
    13681 gatccacgct ctcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg
    13741 tgggtttttg taatgatttg ctcatcgtgg gatctatgct gtgaaatcag cggttattgt
    13801 gcgatatcgg acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta
    13861 ataaataatc gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt
    13921 gctgatattc cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg
    13981 atgctcaagt taaactccac gcttgccgat agccaaccgc agaatcatgt attgtgtctg
    14041 gtgcgactga ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga
    14101 tttttttagc tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat
    14161 ccagacggcg tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca
    14221 cacctgaaga acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg
    14281 tgcctggccc aacgggagcc agcgcggcac cgatctctct ggcgctgaat gccgaaagca
    14341 acaacgtcat gatgctgaca cacgctatca cgcgttacgg catctctacc gacgatccga
    14401 ataaatggcg ttactacctc gattcggtag aagtccacct gccccctttc tgggaacaat
    14461 acatcaacga tgagaatacc gttgaactga tttataccga ttcgctgccg ctggttattt
    14521 cactcaacgg tcatacgctg caggagtaca tgcaggaaac gcgcggctat gccttgcaac
    14581 ctgttccgtc cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca
    14641 atattcgcaa agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag
    14701 cgttgctgat cgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccagatgtat
    14761 ttgttccgtg gctggcaggc ggcgcattac tgctgctggg cgcaggtctg tgggggctat
    14821 tcgcgccccc ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt acaccccgtc
    14881 gttggggatt gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta
    14941 tcgacctggt ctatcccgca cactggcagc catacattgc tcaggatctc ggtcaacaaa
    15001 ccgatattga tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc
    15061 atgatgaagt aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg
    15121 gctcgctgct ggtgctgttt atgctgttat tctggatccc gctggatatg ccgctgaaat
    15181 tcactctctc atggatgaaa ggcgcgcaga ccattgaagc caccagcgta aaacaactgg
    15241 ctgacgctgg cgtgcgggta ggcgatacat tgcgtattag cggtacggga atgtgtaata
    15301 ttcgcacttc cggaacctgg agcgcgaaaa ccaattcacc ttttttaccg tttgactgct
    15361 cgcagatcat ctggaatgac gcccgctcat taccattacc agaatctgaa ctggtcaaca
    15421 aagcgacggc attgactgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat
    15481 ctcgcgtcag tgcctcatta cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg
    15541 attttggcga cattgtactg aagacagcgg atttatgttc tgccaaagat gactgtgtgc
    15601 gactgaaaaa tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac
    15661 gcgccaacgc cgggaagctc gatggcgtga atgtgttatt acgcccggtg agtgcagaat
    15721 cgctggataa cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag
    15781 cggcacaatc actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca
    15841 gcgattttgt tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac
    15901 agtggaacgc tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgaag
    15961 gtatcgtcac aaaaatcttc actgacgcca atggtacgca gcatattggc cttcatccga
    16021 tcccggatcg ttccggcctg tggcgctatc tcagcaccac attgctgcta ctgacgatgc
    16081 tgggtagcgc catttacaat ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc
    16141 gcatgatgga gattcaggcc tattatgaaa gctgcctgaa cccgcaactg atcacccctt
    16201 cagaaagcct tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat
    16261 tgcttcactc tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc
    16321 gttctgctgg atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa
    16381 aagctggtgc ctgaaacatg gggcgccaaa aacggggtta cgccacagga agcgatggaa
    16441 tatatgcgcc agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac
    16501 tggagtgagc aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgtgcc
    16561 gtactgcgtg aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga
    16621 attttgctca ccaatgcgca tccgcacaac ctggcggtaa aacttgagca taccggtctg
    16681 gacgcacacc ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag
    16741 cggttatggc atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt
    16801 gatgacagcg aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc
    16861 gtgactaatc ctgattccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat
    16921 gactaccgcc gcctgatccc ctcgctaatg tgaaggagac gccatgaaag agaaacctgc
    16981 tgttgaggtt cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct
    17041 ggcccgtgaa atgattgaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag
    17101 caaaatcgtc gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt
    17161 gattgtaaag gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga
    17221 agagactgcg gaaagtgtag agaaacgcga aaaaatggcg atggcacgta aacttaatgc
    17281 cttaaccatg ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg
    17341 atttaaacac ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca
    17401 tgaccaatta catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt
    17461 ttcggaaacc ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt
    17521 gctggcagaa ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga
    17581 tatcaccgta cagctgcaag gcgacggtcc gatgaatctg gcggttatta acggtaacaa
    17641 taaccagcaa atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct
    17701 gaaaacgctg gtcggcaatg gttacgtggt gatcaccatt accccgagcg aaggcgaacg
    17761 ctatcagggc gtggttggtc tggaaggtga taccctggcg gcctgcctgg aagattactt
    17821 tatgcgttct gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa
    17881 accggctgca ggcggtatgt tgttgcaggt aatgcctgcg caaaatgccc agcaggacga
    17941 ctttgaccac ctggcgacgc taaccgaaac catcaaaacc gaagaactgc tgaccttacc
    18001 ggcaaacgaa gtgttgtggc gtttgtatca cgaagaagag gtgacggttt acgatccgca
    18061 ggatgtggag ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct
    18121 gcctgatgaa gaagttgata gcatcctggc ggaagatggc gaaattgaca tgcattgtga
    18181 ttactgcggt aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc
    18241 gtctccggca gatccgcaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga
    18301 ttttacgacg taatggcctg atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat
    18361 tattgttggt cagatacagc gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat
    18421 ttccagcgtt tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg tccccagttt
    18481 tccatccttc gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat
    18541 atcaagcgcg tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc
    18601 attaaaagca aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag
    18661 gacgcaggcg tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga
    18721 ccagaatgtc gcatccggtt taacagaacc aggggcaatt aacacatttt cggctaatac
    18781 tttttccgat acctgttcct gaagatgttg ctctccatct aacgccatca gcagatcccg
    18841 cgcgcgtttc gggtctttca taaacccggc atcgcttttc agcgactcaa gcgcagcacg
    18901 tccatagcga ccgctctgct cgagcatgta gatagttacc tggtctgacg tgtttttgcc
    18961 gctctgataa cgcgccatat ggctgttcac gctaatgcgc atactgtcca gcaccggcga
    19021 attaagaagc accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg
    19081 aagaacaacg ggattttgcc ctttacgcca gacgatgctg acaaaatacc ccagcgacca
    19141 caccagtaac accagcaccg ccagcacgcc ctgcaagcgg tcgacagtcc agccgtactg
    19201 agcgacccgc agccataacg cccaggcggc gacgaacaca tacagcggag caaccagcaa
    19261 agcggtttta atcaggcaac gcaacggccc tgtccaggga agtgacgctt tttgcggatc
    19321 gcggacaata gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc
    19381 ggagatgtgg cgagaaatcg cgctcaggcc cgtaaacggc agagtgatga taaacatcag
    19441 ggttagcaat gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat
    19501 agagtcgatt aaacgtgact gtgttcgcgc gaggatcacc gccagcgcgg taaccagacc
    19561 taacgtgaga taaataaacc agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc
    19621 aacaagttta aacaactcac tccacagtaa aagcaccagc cacgtcaagc cattggcgag
    19681 aaaaatcacc agtaatataa gtacgttatg ccatactgac tggtaaaaat agcgatagcg
    19741 gctactgtca ttgcggatgc gaaggctttg ttgtatccac ggtagcaaca acattgccat
    19801 cagcagcaga tagcaaccaa aatcccaaag cgccttttca gctctccagg ggttcatgcc
    19861 atcagtttgc cacttcagcc agccgctcat ccccagcgtg gcaataaaca ccagcgccag
    19921 ccatccccaa aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa
    19981 cgcaacggtc gcaggcacgc cataaacaat ccaactgtga ttttttccgg caagccaggc
    20041 gatcaacagg tagcaaacca gcccctgaag taatccggta gcaatcatcc cccagcgtgt
    20101 tgccggtgaa agttcaacgt tatccatgtt ggttatccag aatcaaaagt gggttaatta
    20161 tcgcatccgg gcagtagtat tttgcttttt tcagaaaata atcaaaaaaa gttagcgtgg
    20221 tgaatcgata ctttaccgat tgaatttgca tcaatttcat taaggaatgc gattccactc
    20281 acaatattcc cgccatataa actaagattt aaccttttga gaacattttc cacacctaaa
    20341 atgctatttc tgcgataata gcaaccgttt cgtgacagga atcacggagt tttttgtcaa
    20401 atatgaattt ctccagatac gtaaatctat gagccttgtc gcggttaaca cccccaaaaa
    20461 gactttacta ttcaggcaat acatattggc taaggagcag tgaaatgcgc gttaacaatg
    20521 gtttgacccc gcaagaactc gaggcttatg gtatcagtga cgtacatgat atcgtttaca
    20581 acccaagcta cgacctgctg tatcaggaag agctcgatcc gagcctgaca ggttatgagc
    20641 gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac cgggatcttc accggtcgtt
    20701 caccaaaaga taagtatatc gtccgtgacg ataccactcg cgatactttc tggtgggcag
    20761 acaaaggcaa aggtaagaac gacaacaaac ctctctctcc ggaaacctgg cagcatctga
    20821 aaggcctggt gaccaggcag ctttccggca aacgtctgtt cgttgtcgac gctttctgtg
    20881 gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac cgaagtggcc tggcaggcgc
    20941 attttgtcaa aaacatgttt attcgcccga gcgatgaaga gctggcaggt ttcaaaccag
    21001 actttatcgt tatgaacggc gcgaagtgca ctaacccgca gtggaaagaa cagggtctga
    21061 actccgaaaa cttcgtggcg tttaacctga ccgagcgcat gcagctgatt ggcggcacct
    21121 ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat gaactacctg ctgccgctga
    21181 aaggtatcgc ttctatgcac tgctccgcca acgttggtga gaaaggcgat gttgcggtgt
    21241 tcttcggcct ttccggcacc ggtaaaacca ccctttccac cgacccgaaa cgtcgcctga
    21301 ttggtgatga cgagcacggc tgggacgatg acggcgtgtt taacttcgaa ggcggctgct
    21361 acgcaaaaac tatcaagctg tcgaaagaag cggaacctga aatctacaac gctatccgtc
    21421 gcgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg cactatcgac tttgatgatg
    21481 gttcaaaaac cgagaacacc cgcgtttctt atccgatcta tcacatcgat aacattgtta
    21541 agccggtttc caaagcgggc cacgcgacta aggttatctt cctgactgct gatgctttcg
    21601 gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac ccagtatcac ttcctctctg
    21661 gcttcaccgc caaactggcc ggtactgagc gtggcatcac cgaaccgacg ccaaccttct
    21721 ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac tcagtacgca gaagtgctgg
    21781 tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt taacactggc tggaacggca
    21841 ctggcaaacg tatctcgatt aaagataccc gcgccattat cgacgccatc ctcaacggtt
    21901 cgctggataa tgcagaaacc ttcactctgc cgatgtttaa cctggcgatc ccaaccgaac
    21961 tgccgggcgt agacacgaag attctcgatc cgcgtaacac ctacgcttct ccggaacagt
    22021 ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga caacttcgat aaatacaccg
    22081 acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa actgtaatga tttgaagctg
    22141 gagaatatct atccagtatc ttatagaaag caaaacggga ggcaccttcg cctcccgttt
    22201 atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag ccaggcgcga
    22261 atggaaagcc cgccccgctc gctggtgcca agctccagca tcccgttatg gttatccacg
    22321 atacgctgca caattgccag ccctaatccc gtgccgctaa tggtgcgcgc actgtcgccg
    22381 cggacaaacg gctggaacag gtgcttacgt tgttccggcg caattcccgg accgtcatct
    22441 tccacctgga accaggcgcg attcggctcc gttccgctgc tgactttgat ccagccattg
    22501 ccataacggg cggcgttgac caccatattc gccaccgcgc gtttgatcga cagcgggtgc
    22561 attttcactt caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc atagccactt
    22621 tcggcagcaa tcacctcacc gagtactgca ttaagatccg ccatttccat cggcatctcc
    22681 tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg cgttgcactc ttcgatatct
    22741 ttattgatcg attctgccag atagccatcc tgctcgctca tcatctcagt cgccaggcga
    22801 atacgcgtca gcggcgtgcg caagtcgtga cttacccccg ccatcagcag cgtgcggtca
    22861 tccgccagtt gcttaacacc agccgccata tggttaaagg cacgggtaac ggaacgcacc
    22921 tccgaagcgc catactcacg cagcggcggc ggaataatcc ctttaccaac ctgcaaggct
    22981 gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa taaacagcca cgccccgcct
    23041 atcgccaata gcataatcgc cagcgtatag cggaacagcg gagagaaatc gccctgatga
    23101 atttcggtca gcggcacgcg tacccagata ttgggcgaca gccaggtttt cagccagacg
    23161 acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag ttgctgtgcc
    23221 atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc ttcggcagcc
    23281 tcgttggagt agagagagat ccccagctca cggtagatct cccgacggaa agcgggaggc
    23341 acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat acgcacttcg
    23401 tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt cagcaccacc
    23461 agataagtcg tcaccaggct ggcgaacagc aaggtgacga tgagcaataa cgtacgggca
    23521 aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc ggtacaaaga
    23581 cgtagcccag gccccaaacg gtctgaatgt aacgcggatg cgctggatct tcttctacca
    23641 tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa tattcacgac
    23701 cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg ctgaccagtg
    23761 ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcatcttcg cggaacattt
    23821 cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct tcctgtgacg
    23881 gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc acgaatacgg gccagcagtt
    23941 cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc aatctccagg cctacgatac
    24001 ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg ctctgactac
    24061 gaagacgtcg gcaaatcgac aagccatctt caccaggtaa cattaaatcc agtaccataa
    24121 gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg acgcttcgaa
    24181 cctggaagcc ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc atgtcgtcat
    24241 cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt tcgcaacaat
    24301 ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct ggtatgaatt
    24361 tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat ttattcggcg
    24421 aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat tatcaaacag
    24481 acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta tgaaaaactc
    24541 aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa aaaggtgacc
    24601 tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa taaaaagcgt
    24661 ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcatggaaaa tctcaaaatc
    24721 gtcgattact cccctcagca ggaaggcaaa gtcttttttg gcgcgtgggt ggagattgaa
    24781 aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat ttttggccgt
    24841 aaagattaca tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa agaagtcggc
    24901 gatctggctg tggtgaatac tcctgccggg gaagcgagct ggtatgttaa tgctatcgag
    24961 tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt tttgccagcc
    25021 agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac catgatgaat
    25081 gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca ggttgacgct
    25141 gccgttcgcc tgcttgacga agggaatacc gtgccgttta tcgcacgtta tcgtaaggaa
    25201 atcaccggcg gtctggatga cacgcagctg cgtaatctgg aaacgcgtct gagctatctg
    25261 cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca tttccgagca aggcaaactc
    25321 accgatgatc tggcgaaggc catcaacgcc accctaagca aaaccgaact cgaagacctc
    25381 tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga agcagggctt
    25441 gagccgttgg ctgacctgct gtggagcgat ccgtcacaca cgccagaagt cgcggctgca
    25501 caatatgttg atgccgataa aggcgtggca gataccaaag ccgcgctgga cggcgcgcgc
    25561 tatatcctga tggaacgttt tgccgaagat gccgcgctgc tggcgaaagt gcgtgattat
    25621 ctgtggaaga acgcgcatct ggtctcaacc gtggtgagcg gtaaagaaga ggaaggggca
    25681 aaattccgcg actatttcga tcatcacgaa ccgttgtcca cggtgccttc tcaccgcgcg
    25741 ctggcgatgt tccgtgggcg taacgaaggc gtactccagc tttcgctgaa tgccgatccg
    25801 cagttcgatg agccgcccaa agagagctat tgcgagcaaa tcatcatgga tcaccttggc
    25861 ctgcgcctga acaatgcccc ggcggatagc tggcgcaaag gcgtagtgag ctggacctgg
    25921 cgcatcaagg tgctgatgca tctggaaacc gaactgatgg gtaccgtgcg cgaacgcgcg
    25981 gaagatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat ggcggcccct
    26041 gccggactgc gtgcaacgat gggcctcgat ccgggtctgc gtactggggt aaaagtggcg
    26101 gtggtcgatg ccactggcaa actggtagcg accgatacca tttacccgca caccggacaa
    26161 gccgcaaaag cagcgatgac tgttgctgcg ctgtgtgaaa agcataacgt tgaactggtg
    26221 gcgatcggca acggtacagc ttcccgcgaa accgaacgtt tctatctcga cgtgcagaag
    26281 cagttcccga aagtgaccgc acagaaagtg atcgtcagcg aagctggcgc gtcggtttac
    26341 tcagcttccg agctggcagc gcaggagttc ccggatctcg acgtttcgct gcgtggtgcg
    26401 gtatctatcg cccgccgttt gcaggatccg ctggcggagc tggtgaaaat cgatccgaaa
    26461 tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcaactggc ccgcaaactg
    26521 gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg atctcaacac cgcttctgtt
    26581 ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat cgtggcctgg
    26641 cgcgatgaga acggccagtt caagaaccgt cagcaactgc tgaaagtcag ccgtctgggg
    26701 ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta accacggtga taacccgctg
    26761 gacgcctcta ccgttcaccc ggaagcctat ccggtggtgg aacgcattct ggcagcaaca
    26821 cagcaggcac tgaaagatct gatgggtaac agcagcgaac tgcgtaacct gaaagcgtct
    26881 gactttactg atgaaaaatt cggtgtgccg acagtaactg acatcatcaa agagctggaa
    26941 aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga tggcgtcgag
    27001 acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg ctgtcaccaa cgtcaccaac
    27061 tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc tggttcacat ctcttcattg
    27121 tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat tgtgaaggtg
    27181 aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgaccatgcg tctggacgag
    27241 cagcctggcg aaaccaacgc ccgtcgcggc ggcggtaatg aacgcccgca aaacaaccgc
    27301 ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg gtaatagcgc gatgatggat
    27361 gcgctggcgg cggcaatggg caaaaaacgt taaacgccct gattgacccc taaacctgcg
    27421 cgataaggtg tccacgccgc atctggcaac gaacgccttg tctgacataa aagtgccgga
    27481 gaatatctcc ggccttttta ttccacaacc aaactcataa tatattccgg caatatttat
    27541 cacttcatta acaactgaaa ccttaattaa acattagcca gtccgggtaa ttcactattc
    27601 gaattatatt ttcgctgcga tataaccttg agccacatca acattgagtc agattattat
    27661 tcaaaccaac attcgcacac attttaagta ttgctgatag aaaccattct cattatcatt
    27721 gtgttgttga ttatttaatc tctcctttgt tggcaaatca tctggtctca tgtcgctgtc
    27781 aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct atgcaataca
    27841 ctccagatac tgcgtggaaa atcactggct tttcccgtga aatcagcccg gcatatcgcc
    27901 aaaaactgct ttctcttggc atgttacctg gctcctcttt taatgtggtg cgcgtcgctc
    27961 cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta cgcaaaaaag
    28021 atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac aacaatgaaa
    28081 aaattaacca taggcttaat tggtaatcca aattctggca agacaacctt atttaaccag
    28141 ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga acgtaaagaa
    28201 gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg cacctattct
    28261 ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca ctacattttg
    28321 agtggcgacg ccgacctgct gattaacgtg gtggatgcgt ctaaccttga gcgtaacctg
    28381 tacctgacgc tacaactgct ggaactcggc attccctgca ttgtggcact gaacatgctc
    28441 gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctgtcggc gcgtcttggc
    28501 tgcccagtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct caagctggcg
    28561 attgatcgct ataaagctaa cgagaatgtg gaactggtgc attacgcaca gccgctactc
    28621 aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa acaacgtcgc
    28681 tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc cggtgaagcg
    28741 tcgcagcatc tggatgccgc cctcgcccgt ctgcgtaatg agatggacga tccggcgctg
    28801 cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt aagcaacacc
    28861 ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct caaccgtttc
    28921 ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc tatcaacatc
    28981 ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg cgctatttgt gcatggtatt
    29041 caatggattg gctacacgct ccacttcccg gactggctga ctatcttcct cgcccagggc
    29101 ctgggtggcg gcattaacac cgtgctgcca ctggtgccgc agattggcat gatgtacctg
    29161 ttcctctcct tccttgagga ctccgggtat atggcgcgtg cggcgtttgt gatggaccgt
    29221 ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc cgttgatcgt cggtttcggt
    29281 tgtaacgtac cgtcggtaat gggtgcacgt acgcttgatg caccgcgtga acgtctgatg
    29341 accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt cgcagtattc
    29401 gcggctgcct tcttcgggca gaacggtgcg ctggcggtct tctcgctgta tatgctgggt
    29461 attgtgatgg cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg cggtgaagcg
    29521 acgccgtttg tcatggagtt gccggtctat catgtaccac acgttaaaag cctgattatc
    29581 cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg gtaaagtgat catcatcgtc
    29641 agcattttcc tgagcgcttt caacagcttc tcgctgagcg ggaaaatcgt cgataacatc
    29701 aacgactcgg cgctggcttc cgtcagccgg gtgatcaccc cggtcttcaa gccgattggc
    29761 gtacatgaag ataactggca ggcaacggtt ggcctgttta cgggcgcaat ggcgaaagaa
    29821 gtggtggtgg gtacgctcaa caccctctac accgcagaaa atattcagga cgaagagttc
    29881 aatccggcgg aattcaacct cggtgaagag ctgttcagtg cggtagatga aacctggcag
    29941 agcctgaaag acaccttcag ccttagcgta ctgatgaacc ccattgaagc tagcaaaggc
    30001 gacggcgaaa tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg tagcgcagct
    30061 gccgcataca gctacctgat tttcgtcctg ctgtatgtac cgtgtatctc ggtgatgggg
    30121 gctatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg ggggctgaat
    30181 atcgcttact cactggcaac attgttctat caggtcgcca gctacagtca gcatccaact
    30241 tacagcctgg tgtgcattct ggcggttatc ctgtttaaca tcgtggttat cggtctgctg
    30301 cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc ggtaagcagt
    30361 tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt caggtgcgcg
    30421 atttgctggc gttacggggc cgtatggaag cggcccagat aagccagaca ttgaacactc
    30481 cacagccaat gattaacgcc atgctgcaac aactggaaag tatgggcaaa gccgtgcgga
    30541 ttcaggaaga acctgacggc tgcctctctg gcagttgtaa aagctgcccg gaaggaaaag
    30601 cctgtctgcg cgagtggtgg gcgctgcgtt aaccttactc catcgcctgt tttggaaagc
    30661 ccggtatgcg tctgcatccg ggcttttttg cgtgcggctt tccataaaaa tgcaactctt
    30721 gcagcacggc gtaagttcct ttgaaagcat ctcgcaggga tgaaaactcg ctaatacaca
    30781 ggagtgaagt ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa cgccatgagc
    30841 aaaaagcaga gttccacccc acacgatgcg ctgttcaaac tctttttacg ccaaccggac
    30901 acggctcgtg attttcttgc gtttcattta ccggcaccca ttcacgcgct ttgtgatatg
    30961 aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga aagctattcc
    31021 gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat acatctattg tctgattgaa
    31081 catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc tattgccgca
    31141 atgcaaaatc accttgatgc cggatacaaa acgttgccga tggtggtgcc attgttgttt
    31201 taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggctggattg tttcgccgat
    31261 cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatgt caccgtcatg
    31321 cctgatgatg aaatcatgca gcaccgacgt atggcgctgc tggagttaat tcaaaaacat
    31381 attcgtcaac gcgacctgat ggggcaggta gagcaaatgg cctgcttatt aagtagtgga
    31441 tacgctaatg acagacaaat caaagggctg tttaattaca tactgcaaac cggcgacgcg
    31501 gtacgtttta acgattttat cgacggcgtt gccgaacgtt caccgaaaca caaggagagt
    31561 ttaatgacaa ttgcggaaag attgcggcag gagggggaac aatccaaagc cctgcatata
    31621 gccaaaataa tgcttgaatc cggagtccct cttacagaca tcatgcgctt taccggggtg
    31681 tcagaagaag agttggctgc ggcgagccag taaatttctg tctcgccatt tcaaaagttg
    31741 cctacaccct ctgcttcaac gccaccagca ggtgacaaaa ctcgaccgga tgcgaaataa
    31801 acggcgcatg ggccgctttg gcgaagatat atgattcgct gtgaggccaa agtttatcca
    31861 gcatcggcac cactttgcgc ggcaccagac cgtcgagata gccatacaat cgcaaaaacg
    31921 gcatgggcac gttttgcagc ggctgacgga gatcgaccgt tttcaggatt tccagcccgc
    31981 cattaagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg
    32041 catcctggcg cgccgtttca gtccccatgg tttgtaacgc caggaaccgc tccactgtac
    32101 gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg
    32161 gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac
    32221 gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc cagcctaacc
    32281 aaatggcttt atcaggtgcc tgttgcagca cggcttcggc catatcagca agtgacagcg
    32341 caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg
    32401 agctaagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat ccgtgcagca
    32461 gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt
    32521 gttctctttt gacttacaag gatgaacata tgctaacagt accgggatta tgctggctat
    32581 gccgaatgcc actggcgtta ggtcattggg ggatttgttc ggtctgctca cgcgctgccc
    32641 gtacagataa aacgttatgc ccacaatgtg gattacccgc cacacactca catcttccct
    32701 gcggtcgctg cctgcaaaaa ccaccgccct ggcaaagact ggtcacggtt gccgactatg
    32761 cgccgccgtt aagtccgctt atccaccagc ttaaattttc ccggcgcagt gaaatcgcca
    32821 gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accgggttgc
    32881 aattgccgga tcgcatcatc agcgttccgt tatggcagcg gcgtcactgg cgtcggggat
    32941 ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gttgcactgc cgatgggata
    33001 gcaaagccgt cacacgaaca cgggccactg cgacccagca ttttctcagt gcccggctac
    33061 gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc cgtgcaaggt cgccatatgg
    33121 tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt
    33181 tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg
    33241 atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt
    33301 atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaatcagga agaagggaca
    33361 caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg tgtttcttat
    33421 tgtccgccgg atgccgtgga agccaccgac actgccctga aatttgacct gctgaccgcg
    33481 tatgttgatg agttaagcgc accatacctg gaagatgcag agatcgactt tgttaccgac
    33541 cagttgggtt ctcagttaac gctgaaagcc ccgaacgcca aaatgcgtaa agtggcagac
    33601 gatgcaccgc tgatggagcg cgtggagtat atgctgcagt cgcagatcaa cccacagctt
    33661 gctggtcacg gtggtcgcgt ttcgctgatg gaaatcaccg aagacggtta cgccattctg
    33721 caatttggcg gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa agaagggatc
    33781 gagaagcagc tgctgaacga attcccggag ctgaaaggtg tgcgcgatct caccgaacac
    33841 cagcgcggcg aacactccta ctactaagtt atcccctctt ttgtagattg cccgatgcga
    33901 cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggca cagcattgct
    33961 ttggcaaatc cctcccttcg tttccccgcg ataatatgac caacctctca taatttaaat
    34021 ttaccccgct ctggtgattc tcaaacgcca gatgttaccc gtatcattca catgggtacc
    34081 aaacatactc ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc
    34141 gcaatatctc tgttcccgat tgggataatt agagtttgtc gtcagaaaat tgacgttacc
    34201 cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc
    34261 ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg
    34321 gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg ctccatcaaa
    34381 gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg
    34441 ggcaaaatgc tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa
    34501 tttggtaaaa agcacatcca gtgggcggtg gtgttaaccg gttttaccgt tggttttgcc
    34561 ctgttctatg aagtgggctt cgtgctgatg ctgcctctgg tgtttaccat cgcggcttcc
    34621 gcgaatattc cactgcttta tgttggtgta ccaatggcgg ctgcactgtc tgtgacccac
    34681 ggcttcctgc caccgcatcc gggcccgact gcgattgcca ccattttcaa tgccgatatg
    34741 ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg
    34801 gtttacgctc gcgtgttgaa aggtatcgat aagccaatcc cggaaggtct ctacagcgcg
    34861 aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg
    34921 ccggtagtac tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct
    34981 ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg ctacgctgat tgccgtgctg
    35041 attgccatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg
    35101 ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg
    35161 ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa
    35221 accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg
    35281 ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg
    35341 acgggtgtta gcccggagct gatggttatt gcggtgggtt ccggtagtgt gattttctct
    35401 catgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag
    35461 accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt
    35521 ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca tccggcatca
    35581 accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac
    35641 ttgttcacgc catcatcggc aaacatcgac tcaagcgttg cggaaagctt gcgtcgccag
    35701 tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct
    35761 tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg
    35821 ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa
    35881 ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga
    35941 ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg
    36001 ctctcccagt aaccgcgtag cgtaggcagg tcatgtgtcg ccgcaaccgc catcgactgc
    36061 tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact
    36121 ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg
    36181 agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc
    36241 agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc
    36301 caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata
    36361 ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc
    36421 attggcggta atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc
    36481 gacgctttca ggcaatatag ttcacggtca caccaggttt ccgccccacc ttccgctacg
    36541 ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag
    36601 caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatca
    36661 cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttctgata catctctggc
    36721 catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca
    36781 aaggctgcct gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggtc
    36841 atctgctcat gatcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt
    36901 agggcggtaa ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt
    36961 tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg
    37021 gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg
    37081 gcactctccg gatttgccgg atagagcgca tgaatcgggt tcaggccgat gaacgacccg
    37141 ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag
    37201 tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc
    37261 agcaacgcct gcggttcgta acagcgtttc ggggcgacaa tcacccggca atgcgcgcgc
    37321 tggtcgtcct gggtgagtgt cagcgtgtga taaccttccg gcagcttcgt cggtagattg
    37381 aacgctttgc cccccgttac atggcctttg tactgtgttc cttcttcggt ggtcagcagc
    37441 cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc
    37501 atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca
    37561 agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtaggc attgatgtaa
    37621 ttggggctaa tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt
    37681 ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta
    37741 aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg
    37801 tacagcacat ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga
    37861 tacggatcgc cgccttgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg
    37921 ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc
    37981 ttccgccatt tcaccgggtc gtagcctttg gccagaatag ccttcacttg ttccacggta
    38041 tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca
    38101 tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa
    38161 gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc
    38221 gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga
    38281 tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag
    38341 tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcggg
    38401 ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg
    38461 tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaatacca
    38521 gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat
    38581 tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg
    38641 ttagcccact ctttttgcag tgatttatcc aacagagccg ccagtgcagg gttgcactgt
    38701 ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc
    38761 tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg
    38821 ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt atcgtgcacc
    38881 accgccagtt tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta
    38941 aagcgagtat taatttcgtt aataatctgc atatggcgcg gcagtaagcc tttcaccagt
    39001 ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt
    39061 ttgctggtaa tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc
    39121 agcagttctg gaatcgcgat agttgggtgg gtatcgttca gctgaataac ttcgtaatcc
    39181 gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa
    39241 caggcacact ggaagtactg ctgcatcaag cgcagctttt taccggcagt atggttgtcg
    39301 tttggataga gaactttggt cagtttttcc gcattgatgc cctgctgttc ggcacgcagg
    39361 aaatcaccgt cgttaaattt agtcagatca aacggatgcg cgtgcgtcgc ctgccacaga
    39421 cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg
    39481 gtaatggtaa actccggctc ccagcgtccg tctttcgtca ctttaccgcc aatccctacc
    39541 tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcggtg ccagtcatcc
    39601 ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc
    39661 agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc
    39721 agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtccgtc
    39781 agattgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac
    39841 aggttgttgc ccgtcaggcg accaatcaaa aactccattg agatgtagtt aacatgtcgc
    39901 tgattcgcca ccggcttggc gaatggctga gcacgcagca tttcggccag tgcttcactc
    39961 actgctagcc accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc
    40021 cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga
    40081 gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg
    40141 accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg
    40201 tgatctcaac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgcaatac
    40261 agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga
    40321 aggtaactct ataaattaag taaaggagtg aaacagtttc ataagtaaaa tatccagtgt
    40381 gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca ctttcatcag
    40441 gaatgaagaa gaaacccttg cttaaatgaa tctgatgaac ataagggaaa ccagtattca
    40501 cgctggatca gcgtcgtttt aggtgagttg ttaataaaga tttggaattg tgacacagtg
    40561 caaattcaga cacataaaaa aacgtcatcg cttgcattag aaaggtttct ggccgacctt
    40621 ataaccatta attacgaagc gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg
    40681 attaactatg ctgattccgt caaaactaag tcgtccggtt cgactcgacc ataccgtggt
    40741 tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac ttccggctgg cgctgatcac
    40801 gagtcctgcg ggctacggaa agaccaccct catttcccag tgggcggcag gcaaaaacga
    40861 tatcggctgg tactcgctgg atgaaggtga taaccagcaa gagcgtttcg ccagctatct
    40921 cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg atatgtgaga cgatggcgca
    40981 aaaacggcaa tatgccagcc tgacgtcact cttcgcccag cttttcattg agctggcgga
    41041 atggcatagc ccactttatc tggtcatcga tgactatcat ctgatcacta atccagtgat
    41101 ccacgagtca atgcgcttct ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt
    41161 gtcacgcaac cttccgcaac tgggcattgc caatctgcgt gttcgtgatc aactgctgga
    41221 aattggcagt cagcaactgg catttaccca tcaggaagcg aagcagtttt ttgattgccg
    41281 tctgtcatcg ccgattgaag ctgcagaaag cagtcggatt tgcgatgacg tttccggttg
    41341 ggcgacggca ctacagctaa tcgccctctc cgcccggcag aatacccact cagcccataa
    41401 gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt tcggattatc tggtcgatga
    41461 ggttttggat aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg
    41521 ctcaatgaac gatgccctca tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgtct
    41581 cgaagagatt gagcgtcagg ggctgttttt acagcggatg gatgataccg gcgagtggtt
    41641 ctgctatcac ccgctgtttg gcaacttcct gcgccagcgc tgccagtggg aactggcggc
    41701 ggagctgccg gaaatccacc gtgccgccgc agaaagctgg atggcccagg gatttcccag
    41761 cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg atattctgct
    41821 taatcacgcc tggagtttgt tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa
    41881 ggccctgccg tgggacagtt tgctggaaaa tccgcagtta gtgttattgc aggcgtggct
    41941 aatgcaaagc caacatcgct atggcgaagt taacaccctg ctagcccgtg ctgaacatga
    42001 aatcaaggac atcagagaag gcaccatgca cgcagaattt aacgctctgc gcgcccaggt
    42061 ggcgattaac gatggtaatc cggatgaagc ggaacggctg gcaaaactgg cactggaaga
    42121 gctgccgccg ggctggttct atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct
    42181 gcactgcaaa ggcgaattga cccgctcact ggcgctaatg cagcaaaccg aacagatggc
    42241 acgccagcac gacgtctggc actacgcctt atggagttta atccagcaaa gtgaaattct
    42301 gtttgcccaa gggttcctgc aaaccgcgtg ggaaacacag gaaaaagcgt tccagctgat
    42361 caacgagcag catctggaac agctgccgat gcatgagttt ctggtgcgca tccgagcaca
    42421 actgttatgg gcctgggcac ggctggatga agccgaagcg tcggcgcgta gcgggattga
    42481 agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg
    42541 ctcgctggcc cgtggtgatt tagataacgc ccgtagccag ctgaaccgtc tggaaaacct
    42601 gctggggaat ggcaaatatc acagcgactg gatctctaac gccaacaaag tccgggtgat
    42661 ttactggcaa atgaccggcg ataaagccgc cgctgccaac tggttgcgtc atacggctaa
    42721 accggagttt gcgaacaacc acttcctgca aggtcaatgg cgcaacattg cccgcgcaca
    42781 aatcttgctg ggcgagtttg aacctgcgga aattgttctc gaagaactca atgaaaatgc
    42841 ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg ttgctgctta atcaactgta
    42901 ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg ctggacgcat taaaactggc
    42961 gaatcgcacc ggatttatca gccattttgt catcgaaggc gaagcgatgg cgcaacaact
    43021 gcgccagctg attcaactca atacgctgcc ggaactggaa cagcatcgcg cacagcgtat
    43081 tctgcgagaa atcaatcaac atcatcggca taaattcgcc catttcgatg agaatttcgt
    43141 tgaacgtctg ctaaatcatc cggaagtgcc agaactgatc cgcaccagcc cgttgaccca
    43201 acgtgaatgg caggtcctgg ggctgattta ctccggttac agcaatgaac aaattgcggg
    43261 cgaactggaa gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt accaaaaact
    43321 cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag aaactgctga agatgatggg
    43381 ttatggggtg tgagtttagc cggataatgc attggatccg gtttacatct ttacatcatt
    43441 caatactcac ccgcgttacg ccatctgttt ctatcaaacc aaaccgcacc ggcaagaaac
    43501 gctccaccac cgcgatattg gtcagcagat ggcatgaagg atgggcgacc ttaaattctc
    43561 ccgcgcccgc cagcgccatc ggcagtacca gttggtcggc gagatattcc cccaccgccg
    43621 ctgggcttgc caggtagcgt ttcacctctt tcactaactg cgcggctacc acctcggcac
    43681 tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat attttcactt tcgacttcca
    43741 gcgagacggt attacccggc ccctggtcgc gcggcaggtt atgaatattc tgttcatgca
    43801 gggaaaaact ccccgccagt gtagcgattt cacgctcagc aacatggcgc ggcacaccag
    43861 ctaatagaac ctctccacgc atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt
    43921 taaacgatgc cacgggcgag acttccgttg ccaccacgcc gcctccggca ggataaaaac
    43981 cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt cgccagcagc ggctccagca
    44041 cccggcggat aaaatccgca ggcggggccg acgggttatc ggtgcctccg ctcacttcaa
    44101 cacgcgaagg tccatcggca aaccacagcg cgggcagcac cgtttgcagc accagcgtac
    44161 agcttccggc actaccgata gcaaagcggt actcgccgcc gcgcacgatg ccaggccgga
    44221 agaccagacg ctgcgacccc agctccgcgc cttccaccgt tgccctacaa atttccgcag
    44281 ccgcttttac cgcggtcaga tgctggcgca acagccccgg tttcgcccgc ccggcacgaa
    44341 tgctggtgat ggtaaatggc tggccggtta tcatcgacag gctcagcgcc gagcgcagga
    44401 tctgcccgcc accttcgccc tgtgcgccat ccagcgcaat catccttttc atcatttatc
    44461 cttttacgca caccacctga cgcagggtat agataacttc caccagatcg ctttgtgccg
    44521 ccatcaccgc atcaatatct ttatacgcca tcgggatttc gtcgatcact tcggcatctt
    44581 tacggcattc cacatgcgcg gtggcacgaa tttgatcttc cacgctgaac agttttttcg
    44641 ctttagtacg gctcattacc cgcccggcac cgtggctgca cgaacagaaa gattcttcat
    44701 ttcccagccc acggacgata aagctttttg ctcccatcga ccctggaata attccatatt
    44761 gaccagcacg cgcagacacc gcgcctttac gcgtcacgta gatctcttca ccaaagtgct
    44821 gttctttttg cacatagttg tggtgacagt tgatctcttc catcgccagc gtttgtggct
    44881 gtctgaccgt tttctgcgta atgctctgca atgccgttac cacgttttcc atcatcgcat
    44941 cgcggttaag gctggcaaaa agctgcgccc aggccacggc tttcaggtaa tcatcaaagt
    45001 attccgtacc ttccataaag tacgccagat cacgcgacgg caacgtctca agcgtttcct
    45061 gcatctcttt ttgtgccaga tcgataaagt aagtcccgat ggcgttacca attccgcgtg
    45121 aaccggagtg cagcataatc cacacctggt ccgactcatc aaggcagatt tcaataaagt
    45181 ggttaccggt tcccagcgtt cccaggtgtt tatagttatt ggtattcagg aaacggggat
    45241 atttttgcgt taaccactga taaccggctt caagctcagc ccatttcgca tcgacgttaa
    45301 caggcgggtt ttcccatgcg cctttatcac gtttacaacg gccagtggta cgcccgtgcg
    45361 gcacggccat ttcaatcgcc tgacgcagct ctgccaggtt ttcaggcagg tcttccgccg
    45421 ttaacgcggt acgcagcgcg ttcattccac agccaatatc cacgcccacc gccgccggaa
    45481 taatcgcccc tttggtcggg atcacgctac caatggtgga acctttaccc aggtgtacat
    45541 caggcattac cgcaatatgt ttgaaaataa acggcatctt cgccgtatta ataagttgct
    45601 gacgcgcatc ggcttctacc ggcacgcctt tggtccacat ttttaccggg gcattttcag
    45661 tggtcagtaa ttcgtaattc attttgtttt tctcttttcg ttgtttgctg tcgtg
//
LOCUS       DAZFWP020000016        44112 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFWP020000016 DAZFWP020000000
VERSION     DAZFWP020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.016"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            201..872
                     /locus_tag="ACHK0Z_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             201..872
                     /locus_tag="ACHK0Z_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HGU4500915.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            882..2078
                     /gene="yaiP"
                     /locus_tag="ACHK0Z_RS04250"
                     /old_locus_tag="pgaptmp_000850"
     CDS             882..2078
                     /gene="yaiP"
                     /locus_tag="ACHK0Z_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4500916.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2056..2637
                     /locus_tag="ACHK0Z_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             2056..2637
                     /locus_tag="ACHK0Z_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4500917.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2639..3412
                     /gene="yaiO"
                     /locus_tag="ACHK0Z_RS04260"
                     /old_locus_tag="pgaptmp_000852"
     CDS             2639..3412
                     /gene="yaiO"
                     /locus_tag="ACHK0Z_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGU4500918.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3597..3872
                     /gene="frmR"
                     /locus_tag="ACHK0Z_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             3597..3872
                     /gene="frmR"
                     /locus_tag="ACHK0Z_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU4500919.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3907..5016
                     /gene="frmA"
                     /locus_tag="ACHK0Z_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             3907..5016
                     /gene="frmA"
                     /locus_tag="ACHK0Z_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU4500920.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5109..5942
                     /gene="fghA"
                     /locus_tag="ACHK0Z_RS04275"
                     /old_locus_tag="pgaptmp_000855"
     CDS             5109..5942
                     /gene="fghA"
                     /locus_tag="ACHK0Z_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4500921.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQDAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIHYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6067..6606)
                     /gene="yaiL"
                     /locus_tag="ACHK0Z_RS04280"
                     /old_locus_tag="pgaptmp_000856"
     CDS             complement(6067..6606)
                     /gene="yaiL"
                     /locus_tag="ACHK0Z_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU4500922.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6708..7919)
                     /gene="mhpT"
                     /locus_tag="ACHK0Z_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             complement(6708..7919)
                     /gene="mhpT"
                     /locus_tag="ACHK0Z_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU4500923.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8194..9207)
                     /gene="mhpE"
                     /locus_tag="ACHK0Z_RS04290"
                     /old_locus_tag="pgaptmp_000858"
     CDS             complement(8194..9207)
                     /gene="mhpE"
                     /locus_tag="ACHK0Z_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU4500924.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9204..10154)
                     /gene="mhpF"
                     /locus_tag="ACHK0Z_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             complement(9204..10154)
                     /gene="mhpF"
                     /locus_tag="ACHK0Z_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU4500925.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10151..10960)
                     /gene="mhpD"
                     /locus_tag="ACHK0Z_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             complement(10151..10960)
                     /gene="mhpD"
                     /locus_tag="ACHK0Z_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU4500926.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10970..11836)
                     /gene="mhpC"
                     /locus_tag="ACHK0Z_RS04305"
                     /old_locus_tag="pgaptmp_000861"
     CDS             complement(10970..11836)
                     /gene="mhpC"
                     /locus_tag="ACHK0Z_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU4500927.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11854..12798)
                     /gene="mhpB"
                     /locus_tag="ACHK0Z_RS04310"
                     /old_locus_tag="pgaptmp_000862"
     CDS             complement(11854..12798)
                     /gene="mhpB"
                     /locus_tag="ACHK0Z_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU4500928.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12800..14464)
                     /gene="mhpA"
                     /locus_tag="ACHK0Z_RS04315"
                     /old_locus_tag="pgaptmp_000863"
     CDS             complement(12800..14464)
                     /gene="mhpA"
                     /locus_tag="ACHK0Z_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU4500929.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14655..15488
                     /gene="mhpR"
                     /locus_tag="ACHK0Z_RS04320"
                     /old_locus_tag="pgaptmp_000864"
     CDS             14655..15488
                     /gene="mhpR"
                     /locus_tag="ACHK0Z_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU4500930.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRPMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15565..16647
                     /gene="lacI"
                     /locus_tag="ACHK0Z_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             15565..16647
                     /gene="lacI"
                     /locus_tag="ACHK0Z_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU4500931.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16770..19844
                     /gene="lacZ"
                     /locus_tag="ACHK0Z_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             16770..19844
                     /gene="lacZ"
                     /locus_tag="ACHK0Z_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU4500932.1"
                     /translation="MTMITDSLVVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWNKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAANLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFIRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGSHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19896..21149
                     /gene="lacY"
                     /locus_tag="ACHK0Z_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             19896..21149
                     /gene="lacY"
                     /locus_tag="ACHK0Z_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU4500933.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21215..21826
                     /gene="lacA"
                     /locus_tag="ACHK0Z_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             21215..21826
                     /gene="lacA"
                     /locus_tag="ACHK0Z_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU4500934.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(21929..23110)
                     /gene="cynX"
                     /locus_tag="ACHK0Z_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             complement(21929..23110)
                     /gene="cynX"
                     /locus_tag="ACHK0Z_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU4500935.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     SWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23116..23586)
                     /gene="cynS"
                     /locus_tag="ACHK0Z_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             complement(23116..23586)
                     /gene="cynS"
                     /locus_tag="ACHK0Z_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU4500936.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23617..24276)
                     /gene="cynT"
                     /locus_tag="ACHK0Z_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     CDS             complement(23617..24276)
                     /gene="cynT"
                     /locus_tag="ACHK0Z_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU4500937.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24386..25285
                     /gene="cynR"
                     /locus_tag="ACHK0Z_RS04360"
                     /old_locus_tag="pgaptmp_000872"
     CDS             24386..25285
                     /gene="cynR"
                     /locus_tag="ACHK0Z_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU4500938.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALHELGAGKRAIHDVAALTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25325..26608)
                     /gene="codA"
                     /locus_tag="ACHK0Z_RS04365"
                     /old_locus_tag="pgaptmp_000873"
     CDS             complement(25325..26608)
                     /gene="codA"
                     /locus_tag="ACHK0Z_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU4500939.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26598..27857)
                     /gene="codB"
                     /locus_tag="ACHK0Z_RS04370"
                     /old_locus_tag="pgaptmp_000874"
     CDS             complement(26598..27857)
                     /gene="codB"
                     /locus_tag="ACHK0Z_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU4500940.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(28100..29986)
                     /gene="prpE"
                     /locus_tag="ACHK0Z_RS04375"
                     /old_locus_tag="pgaptmp_000875"
     CDS             complement(28100..29986)
                     /gene="prpE"
                     /locus_tag="ACHK0Z_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU4500941.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(30026..31477)
                     /gene="prpD"
                     /locus_tag="ACHK0Z_RS04380"
                     /old_locus_tag="pgaptmp_000876"
     CDS             complement(30026..31477)
                     /gene="prpD"
                     /locus_tag="ACHK0Z_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU4500942.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31511..32680)
                     /gene="prpC"
                     /locus_tag="ACHK0Z_RS04385"
                     /old_locus_tag="pgaptmp_000877"
     CDS             complement(31511..32680)
                     /gene="prpC"
                     /locus_tag="ACHK0Z_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU4500943.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(32725..33615)
                     /gene="prpB"
                     /locus_tag="ACHK0Z_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             complement(32725..33615)
                     /gene="prpB"
                     /locus_tag="ACHK0Z_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU4500944.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33854..35440
                     /gene="prpR"
                     /locus_tag="ACHK0Z_RS04395"
                     /old_locus_tag="pgaptmp_000879"
     CDS             33854..35440
                     /gene="prpR"
                     /locus_tag="ACHK0Z_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU4500945.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35538..35813)
                     /gene="yahO"
                     /locus_tag="ACHK0Z_RS04400"
                     /old_locus_tag="pgaptmp_000880"
     CDS             complement(35538..35813)
                     /gene="yahO"
                     /locus_tag="ACHK0Z_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU4500946.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35960..36631
                     /gene="yahN"
                     /locus_tag="ACHK0Z_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             35960..36631
                     /gene="yahN"
                     /locus_tag="ACHK0Z_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU4500947.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36648..36893)
                     /gene="yahM"
                     /locus_tag="ACHK0Z_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             complement(36648..36893)
                     /gene="yahM"
                     /locus_tag="ACHK0Z_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HGU4500948.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(37306..38121)
                     /gene="yahL"
                     /locus_tag="ACHK0Z_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             complement(37306..38121)
                     /gene="yahL"
                     /locus_tag="ACHK0Z_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU4500949.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38364..39413)
                     /gene="yahK"
                     /locus_tag="ACHK0Z_RS04420"
                     /old_locus_tag="pgaptmp_000884"
     CDS             complement(38364..39413)
                     /gene="yahK"
                     /locus_tag="ACHK0Z_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU4500950.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39500..40456)
                     /locus_tag="ACHK0Z_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             complement(39500..40456)
                     /locus_tag="ACHK0Z_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4500951.1"
                     /translation="MKKSWRNNVEFYLIGLLALTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(40453..41424)
                     /locus_tag="ACHK0Z_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             complement(40453..41424)
                     /locus_tag="ACHK0Z_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4500952.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(41417..42901)
                     /locus_tag="ACHK0Z_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             complement(41417..42901)
                     /locus_tag="ACHK0Z_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4500953.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42950..43933)
                     /locus_tag="ACHK0Z_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             complement(42950..43933)
                     /locus_tag="ACHK0Z_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4500954.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
BASE COUNT        10642 a        11947 c        11198 g        10325 t
ORIGIN      
        1 tacataccaa acatacaaac acttaacata tgaattgcat agaaacatta cccagtcgaa
       61 cctccgggtc tgaaagccct tgcttttcag ttattagcaa catttaacat ttataaatta
      121 acatctggct gcgccagaga ttacgtctct gagtgcatcg ttgtgcttgt agtataataa
      181 ataaaatgga gtgtcattaa gtggataagg ttttagattc agccctcctt tcttcggcaa
      241 ataaaagaaa gggtatcctt gctattggcg cacatcctga cgatatagaa ttaggctgtg
      301 gcgcatcgct tgctcgtctt gcgcaaaaag gaatttatat cgcagccgtg gtgatgacta
      361 ccggcaactc tggcacagat ggaataatcg atcgccatga agaatcgcgc aacgccctaa
      421 agatattagg gtgccaccaa actattcatc ttaattttgc tgacacccgc gctcatttac
      481 agctcaatga tatgatttcc gccctggaag acatcattaa aaatcaaatt ccttctgatg
      541 ttgaaatcat gcgggtatat accatgcatg atgccgaccg ccatcaggat catctcgctg
      601 tttatcaagc ttcaatggtt gcctgccgca ctattccaca aattctcggc tacgaaaccc
      661 caagtacctg gctttcattt atgcctcagg tttttgaatc cgttaaagaa gaatatttca
      721 cagtcaaact tgctgcatta aaaaaacata aaagccagga acgacgcgat tatatgcgtc
      781 atgatcgcct gcgtgcagtt gcacaatttc gcgggcaaca ggtcaatagc gatctgggtg
      841 aaggctttgt aattcataaa atgattcttt gagcaaattg catgaaaacc tggatattta
      901 tctgtatgtc catagcaatg ttgctatggt ttttaagtac gctaagacgt aaacccagtc
      961 aaaagaaagg ctgtattgac gccattatac ctgcgtataa cgaaggcccg tgtctggcgc
     1021 agtcactgga taatctactg cgtaaccctt atttttgccg ggtaatttgc gttaacgacg
     1081 gctccacgga caataccgaa gcggtcatgg cggaagtcaa acgcaaatgg ggcgaccgct
     1141 ttgttgccgt cacgcaaaaa aataccggta aaggtggtgc gctgatgaat ggcctcaatt
     1201 atgccacctg cgaccaggtt tttttaagtg atgccgacac ctatgttccg cccgatcaag
     1261 acggaatggg ctatatgctg gcagaaattg agcgcggtgc tgatgccgta ggcggcattc
     1321 cctctactgc gttgaaaggc gcggggctgt taccgcacat ccgcgcgacc gtaaagttgc
     1381 cgatgattgt tatgaagcgc acgctacagc agctcctggg cggcgcaccg tttattatca
     1441 gcggtgcctg cggaatgttc cgcactgatg tattgcgtaa gttcggtttc tcagatcgta
     1501 ctaaagtcga agaccttgat ctcacctgga cattagtggc aaacggctac cgtattcggc
     1561 aggcgaatcg ctgcatcgta tacccacagg aatgcaacag cccgcgtgag gagtggcgtc
     1621 gctggcggcg ttggattgtg ggatacgcgg tctgtatgcg cctgcataaa agacttttat
     1681 ttagccgctt cggtatcttc agtatatttc ctatgctgtt ggttgtgatt tatggcgttg
     1741 ggatttatct cactacctgg tttaatgaat tcatcaccac cgggccgcat ggtgtggtgt
     1801 tggcaatgtt tccgcttatc tggatcggcg tagtttgtgt tattggtgct tttagcgcct
     1861 ggtttcatcg ttgctggttg ttggtgcctt tagcgccgct ttccgttgtg tatgtattat
     1921 tagcttatgc catctggatt atttatggac ttattgcctt ttttactgga cgcgaacctc
     1981 agcgcgacaa acccacccgc tattccgcac tggtggaagc gtcaaccgct tattcccaac
     2041 cttctgtcac aggaactgaa aaactatctg aagcttaatg ttaaagagaa aaatattcag
     2101 attgcagacc aggtaattat tgatgaaact gcaggtgaag ttgttatcgg cgcgaatacc
     2161 cgtatttgtc atggtgccgt tattcagggt ccggtagtga ttggcgcaaa ctgcctgata
     2221 ggtaattatg cgtttattcg tcctggcaca ataatcagca atggtgtaaa aattggtttt
     2281 gccaccgaaa ttaaaaatgc ggttattgaa gcggaagcaa cgattggtcc gcaatgtttt
     2341 attgccgact cggtagttgc aaaccaggca tatttgggcg cacaagtacg taccagtaat
     2401 catcgtctgg atgaacagcc tgtgtctgtt cgaactccag agggaattat cgctaccgga
     2461 tgcgataaat taggttgtta tatcgggcag cgttcacgcc ttggcgtaca agttataatt
     2521 ttgcctgggc gaattatttc cccgaacacg caacttggcc cgcgcgtgat tgtagaacgt
     2581 aatttacctg ccggaactta ttcactccga caagaactta tccgtacagg agattaaaat
     2641 gataaaacgg acgttattag cggcggccat ttttagcgca ttgcccgctt atgccgggtt
     2701 aacttccatt accgcgggct acgattttac cgattattct ggcgatcatg gcaaccgtaa
     2761 tttagcgtat gctgaactgg tggcgaaagt tgaaaacgca acgctgcttt ttaatctttc
     2821 ccaggggcgt cgtgattatg aaactgaaca tttcaatgcc actcgcggtc agggtgcggt
     2881 ctggtataaa tggaataact ggctgacaac ccgaacgggt attgcctttg cggataatac
     2941 gccggtcttt gcccgccagg attttcgtca ggatattaac ctggccctgt tgccaaaaac
     3001 gcttttcacg accggttatc gctacactaa atattacgat gatgtcgaag tcgatgcctg
     3061 gcaaggcggc gtatcactct atactggccc ggtgatcacc agctaccgct atacccatta
     3121 tgactccagc gatgcaggtg gtagttatag caatatgatt tccgtgcgtc tgaatgaccc
     3181 gcgcggcact ggttatacgc aactatggct aagccgcgga acaggcgctt acacctatga
     3241 ctggacgcca gaaacacgct acggcagcat gaagagcgtc agtttgcaac gtattcaacc
     3301 gctgactgag caacttaatc tcgggctgac ggcaggtaaa gtgtggtacg acaccccaac
     3361 cgatgattat aacggtctgc aacttgcagc ccatctgacc tggaaattct gattccttct
     3421 gccgcccgct atccggggcg gccttccctg ccgattagcc ccccctttcc tctttgtttt
     3481 ccgaccacat tcaccggata aattttattc tccagtgtta tatactatag gggggtatgc
     3541 attgacatat agcatacccc cctatagtat attgcgtgca gatgatgagg tgcgaaatgc
     3601 ccagtactcc ggaagagaag aaaaaggtcc ttactcgagt tcgtcgtatt cgagggcaga
     3661 ttgatgctct ggaacggtcg ctggagggtg atgccgaatg ccgtgccata ctccaacaga
     3721 tcgctgccgt tcggggcgcg gctaacgggc tgatggcaga agtgcttgaa agccatatcc
     3781 gggaaacgtt tgaccgaaat gactgctaca gccgcgaagt cagccaatcc gttgacgaca
     3841 ctattgaact ggttcgagcc tatcttaaat agctgaatct attaccatat tgaggaagag
     3901 cgagagatga aatcacgtgc tgccgttgca tttgctcccg gtaaaccgct ggaaatcgtt
     3961 gaaattgacg ttgcaccacc gaaaaaaggt gaagtgctga ttaaagtcac ccataccggc
     4021 gtttgccata ccgacgcatt taccctctcc ggggatgacc cggaaggtgt attcccggtg
     4081 gttctcggtc acgaaggggc cggcgttgtg gttgaagtcg gtgaaggcgt aaccagcgtc
     4141 aaacctggcg accatgtgat cccgctttac accgcggagt gcggcgagtg tgagttctgt
     4201 cgttctggca aaactaacct ctgtgttgcg gttcgcgaaa cccagggtaa aggcttgatg
     4261 ccagacggca ccacccgttt ttcttacaac gggcagccgc tttatcacta catggggtgc
     4321 tctacattca gtgaatacac cgtggtcgcg gaagtgtctc tggccaaaat taatccagaa
     4381 gcaaaccatg aacacgtctg tctgctgggc tgtggcgtga ccaccggtat tggcgcggtg
     4441 cacaacacag ctaaagtcca gccaggtgat tctgttgccg tgtttggtct tggcgcgatt
     4501 ggtctggcag tggttcaggg cgcgcgtcag gcgaaagcgg gacggattat cgctatcgat
     4561 accaacccga agaaattcga tctggcgcgt cgcttcggtg ctaccgactg cattaacccg
     4621 aatgactacg acaaaccgat taaagatgtc ctgctggata tcaacaaatg gggtatcgac
     4681 catacctttg aatgcatcgg taacgtcaac gtgatgcgtg cggcgctgga aagtgcgcac
     4741 cgcggctggg gtcagtcggt gatcatcggg gtggcaggtt ctggtcagga aatctccacc
     4801 cgtccattcc agttggtcac tggtcgcgta tggaaaggtt ccgcgtttgg cggcgtgaaa
     4861 ggtcgttccc agttaccggg tatggttgaa gatgcgatga aaggtgatat cgatctggaa
     4921 ccgtttgtca cgcataccat gagccttgat gaaattaatg acgccttcga cctgatgcat
     4981 gaaggcaaat ccattcgaac cgtaattcgt tactgatttc ccgcaggtat accccgtcca
     5041 cttcagacgg ggttcttaat actctccccg ggcagccgtc cgggggatta accgtgagat
     5101 aatgactgat ggaactcatt gaaaaacatg ccagctttgg cggctggcaa aatgtgtatc
     5161 ggcattattc ccaatcactg aaatgtgaaa tgaatgtcgg cgtctatctc ccaccaaaag
     5221 ccgcgaatga aaaattgccg gtgctgtact ggctttcggg cctgacctgc aacgagcaga
     5281 atttcattac taaatcgggg atgcagcgtt acgcggctga acacaacatt attgttgttg
     5341 cgccggacac cagtccgcga ggcagtcatg tcgcagatgc cgaccgttac gatctcgggc
     5401 aagatgccgg gttttacctg aacgcaacgc aagcgccgtg gaatgaacat tacaaaatgt
     5461 atgactatat ccgcaacgag ctgccggatt tagtgatgca acattttccg gcaacgacca
     5521 gaaagtctat ctctggtcat tctatgggcg ggctgggcgc gctggtgctg gcgttacgta
     5581 acccagatga atatgtcagc gtctcggcgt tttcgcccat tgtctcccca tcgcaagtac
     5641 cgtggggaca gcaagccttt gccgcatatc ttggtgaaaa taaagatgcc tggttgaatt
     5701 acgatccggt gagtcttatt tcacaaggtc aacgcgttgc ggaaatcatg gttgatcagg
     5761 ggttgagtga tgatttttac gcggaacagc tgcggactcc aaatcttgaa aagatctgcc
     5821 aggagatgaa tatcaagacg ttaatccatt atcacgaggg ttacgaccac agctattatt
     5881 ttgtctccag ttttattggc gagcatattg cctaccacgc caataaactg aatatgcgtt
     5941 gataatagtg cacgactgcc ggatgcggcg tgaacgcctt atccgcctac atttcgcccg
     6001 caggccgtag gcatgataag acgcgcatag cgtcgcatca ggcatctgcg cacgacttat
     6061 ctatgtttac cacatcaaat catcaggcac tttgaagtcg gcatatgggt cgtcttcatc
     6121 ctgctcttcg gcactgagcg cgctgtgcaa cacaatactg ctggcatcac gctgagcaat
     6181 tttatcggca acgctggcgg gaataatggc gtattcacct tcgctattgt tatcgaccaa
     6241 caagcgggca atcgccagac gaccattaat caactgcgcc tgtgtgagct tatcgacaaa
     6301 aatcttctta atcagattgc cgtcagtgaa gttaaaacca atatcaccat tggcaatggt
     6361 gattcggttc atttcaataa gctgcttcac ctgggcttta tattctttcg ccaacgccgc
     6421 ttgtttttgc tgttcgctaa gctgtttatc acgctcaagc tgtgcctttt tattttcttc
     6481 taccgccgcc cgagcttcac gcgcctgaac gcgcgatttt ttcgccgttc tctcgacctt
     6541 cgccgctttt ttgctggtga ctaatcctgc tttgagcaac tgctcttgta aggtaagttt
     6601 tgccatcttt gtttccaaac ccgctgaata atttttagcg agtatacctg taaacgccgt
     6661 gggtgtattg ggggcaagtc cctttcgccc ctgctaaata aggcgcgtca ggcatcggca
     6721 cacggctgca ttcttgatct ccgactcatc aaaataaaca ccgccaaccc agcaacaaga
     6781 atacccggtg cagaagcggc cattacgccg accgtgccag tgcctaatgc cagcattttc
     6841 ccggccagta acggaccgct catagccccc agacgcccta ccgccacggc tgttcccaca
     6901 cctgttgcgc ggatctgcga actgtaaaac aacggtgcca gggcatacaa aacgctttgc
     6961 ccacctgtcg caaacaaccc cgcgacaaat cccgccagca acataccgtt aaatgacgac
     7021 accgttccaa gcgccagcag cgaagctaac atgccgctat aaatcagtag cgacatggtt
     7081 actggacgca gcttatccat caatgcgccc aacattaacg tcccgcttgc cgcccccatt
     7141 tgcagggcaa acatcacccc tgccgcctgc gatggctgga atccttgctc caccaaaagt
     7201 agcggtagcc agttgatcaa catataaacc accagcagag tgaagaaata acacaaccac
     7261 agcagcagcg tcgcggttgc cgtttctggc gcaaataagg cacgcagtgg tggcgcagcc
     7321 tgtttttcgc cagcgaaaac cgctgactcc ggcagccagc gcattaatag cggcaccaga
     7381 atcaacggca ccacaccacc tacccaaaac accgtttgcc atgctaagtt tgcccccgcg
     7441 aaacccagtg tcgccgccag cgccgcgcca atgggaacac cgcaatacat caggctcact
     7501 gccgtcccac gaaaacgtgg acccgcggct tcagacgtca gggcgataag attcggcaac
     7561 gccgccccca gcccgacacc ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag
     7621 gcaatcgccg ttgccagtga gaacaaacca aacagcgcaa ctgagccaat caaaatactc
     7681 ttacgaccat aacggtctgc cagcatcccg ccaaccaacg cgccgggtag caaaccgagt
     7741 attccggcgc taaatatcca gcccatttgc attttatcga gtgcgaaagc ctgggcgatg
     7801 ccacccgccg caatgccagc cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa
     7861 caaagcccga tggtcagcat caggcgggat gaagatgatg aaggggtacg agtcgacata
     7921 agatgaacct cgttgcgagt gcaggtgaat cgcgactgat acattgccag ccgcgtaatc
     7981 agaactattt aatgactgcc tacgggcgaa atgtaggccg gacaagacgt ttatcgcatc
     8041 cggcagtcgt gtgcagatgc ctgatgcgac gctaacgcgt cttatcaggc ctacaaattc
     8101 tcacgccacc cgtaggccgg ataaggtgtt tacgccgcat ccggcagtcg tgctgggatg
     8161 ggcatacaaa atgcacgttc tcggcagtca tcattatttg ttgttgcgca gatccagcgc
     8221 cacgtcaacg atcatatcct cctggccgcc aaccatccgg cgtttgccca gctcaacgag
     8281 aatatccacc gcacttaagc cataacgcgc cgccgccgtt tcacagtgac gcaggaagct
     8341 cgagtaaaca ccagcgtatc ccagcgccag cgtttcgcga tcgactcgta ccggtcgatc
     8401 ctgcaacgga cgcaccaggt cgtcagcggc atccattaac gcatagagat cggtcccatg
     8461 ctgccagccc agtttatccg cggcggcaat aaacacttcc agcggtgcgt tacctgcgcc
     8521 cgcgcccatt cccgcgaggc tggcgtcgat tcggtcgcag ccctcttcca ccgccgcgat
     8581 agagttcgcc acgccaagac tcaggttatg gtgagcgtgc atgccagttt gcgtttctgg
     8641 tttcagctct gctttcaggg cgcggaaacg gtcacggata tcgctcatgt tcatcgcacc
     8701 gccagaatcc accacataaa tacaggtcgc accgtagcct tccatcagct ttgcctgctt
     8761 tgcgagattc tccggcgtgg tcatatggct catcatcaga aaaccaacgg tgtccattcc
     8821 gagctcgcgg gcatactgaa tatgctgggc ggaaacatca gcttcggtac agtgcgttgc
     8881 cacacgaacc acccgcgcgc cagcctgcca ggcatttttc agatcgtgaa tagtgccgat
     8941 tcctggcagc aacaacgtcg cgattttggc gtgcttcacc acatccgccg ctgcttcaat
     9001 ccattcaagg tcgctatgtg cgccgaaacc atagttaaag ctggaaccct gcaaaccgtc
     9061 gccatgggcc acttcaatcg aatccacgcg ggcatcgtcc agtgctttgg caatctggcg
     9121 aacgttttcc agcgaatact gatgacgaat ggcgtgcata ccgtcacgca acgtgacgtc
     9181 cgagatataa agttttttac cgttcatgcc gcttctcctg ccttgcgcgc cagtgactgg
     9241 gccatttttt ccgctgtcgc cagcgcactg gaagtcataa tgtcgaggtt gcccgcatag
     9301 gcaggcagat aatgcgctgc gccttcgact tccagccaga ccgctgtttt cagtccggag
     9361 aattgcccca cgcccggtaa attgaccggt ttatcctgcg ggataacttc aaactgcacg
     9421 cgctgtttca ggcgataacc cggtacgtaa gcctgcaccg cctcagccat ttcattgatt
     9481 gaggcttcga tatcatcttg tgaagcttcg tcgctcaata catacaccgt gtcacgcatc
     9541 atcaacggtg gctctgctgg gttaagcaca ataatcgcct tcccttttgc cgcgccgccc
     9601 accacttcaa tggctcggga agtggtttcc gtaaattcat cgatattggc acgcgtgcca
     9661 ggtccggcag atttactggc gatagaagcg ataatttcgg cgtaatgaac acgcgccacg
     9721 cgtgaaactg ccgccaccat tggaatggtg gcctggccgc cgcaggtgac catgttgacg
     9781 ttcagttgat cgacgttcgc ctcgaggtta accaccggca cgcagtaagg gccgatggca
     9841 gcaggcgtca ggtcaattaa gcgaatatcc ggtttcgctt cgcgtaaagc ggcatcgttt
     9901 ttcacatgcg cgcccgcgct ggtcgcatca aatacaatgt cgatatcagc aaattcaggc
     9961 atgttcatca gcccgatcac cccttcatgg gtggtggcga cgcccatacg tctggcacgc
    10021 gccagaccgt cggactgagg atcaatgccg accatcaccg ccatctccag atgctgaccg
    10081 tgacgcaaaa ttttaatcat cagatcggtg ccaatgttgc cagaaccgat aatggcgact
    10141 ttacgcttac tcatgacaga cttccttttg gggctgcgct tgaaaatgtc gcagcaactg
    10201 aacctatgcc ttcaatatgg gcttcaaaac gatcgcccgc attcactgcc accatcggac
    10261 ctaatgcccc ggtaagaatg atatctccgg cgcgcagcgg ttcacccagg ctggccattt
    10321 tgcgtgccag ccagacggcg gcattaagcg gatgtcccag gcattcgctg ccgcgcccgc
    10381 tggaaacctc ttcgttatta cgcgtcatct tcatggcgca gtttttcagg tctaaccccg
    10441 ccggacgttg cgccggaccg ccgatgacat acaccccgca ggaagcgtta tctgccacgg
    10501 tatcgacaaa ctgaatcgac cagtcgcgaa tgcggctacc caccacctcc agcgccggaa
    10561 gtacccattc aatggcgtta tacagttcgt cgaaggtgat atcggttgcg ggcaaatcgc
    10621 ggttcaacac cagtgcgatc tccgcttcaa tgcgggcttg cagaacacgg gaaaaaggaa
    10681 tgatttcgtt atcgccataa cacatgtcgg caaataatgt cccaaaatcc ggttgatcaa
    10741 cgcccagttg ttgctgcact ttcggatgcg tcaggcccac tttacgccct accacgcgac
    10801 gcccctgcgc aacgtcatat tgcacattta tgtgctgaat ggcgtaggcc gcttcagcgt
    10861 tatcgatacc aatcagatcg cgcagcggcg caatcgcttc gccctgctct gcggcgcggc
    10921 gtaaatccgc cgccagttgc tcaagagtat gcttcgtcat gaccattcct taagggcgtg
    10981 cgaggaaatt cagcaccaat tgattgaaag cgtcggcatg ttcccactgc gcccagtgac
    11041 cacagtcgcg gaagatatgc agttcagaac cggcaatgcc ggacagcaga cgcagacccg
    11101 catccatcgg cacaaagcgg tcgttgcgcc cccagacaat cagggtttgc gctttgattt
    11161 ccgccagacg tgggccaaaa tccgggaact gtttcggatt agcttccagg ctcttaacga
    11221 agttttccag gtgatcgcgg cgcgacagca tattattcag gcgcgcttca aacagggcgt
    11281 cggtcaaatc gctggtatcg aaaacgaaga tatccatcat cagcttcagg ttttcgatag
    11341 tcggctgacg ataaagctga ttcagtcgct taataccttc ggttggcatc ggcgtaaaca
    11401 aactcatgcc gcccgtaccg ccgcccatca gcaccagttt gccgacgcgc tccggccagt
    11461 taagggtgaa cgccatagaa ctatggccgc ccatcgagtt gcccagcagg tggattttgg
    11521 cgatatccag ttgatccacc acgcttttca ggattcgtgc attaagatcc gatcgtgaac
    11581 cactattaac gatcgaatcg cttttgcccc aacccggaca atccagcagg attacccgat
    11641 agcccgcctc taccagcgga tcgatattgc ggctgaagtt cgcccagcca gtagcacccg
    11701 ggccggaacc gtgcagcagg acaacggttt cgtcgccttg tccgcagtca ttaaaatgga
    11761 tgcgcagcgt tttacccgct tcttctacat tcagaaaacg gctggtggcg gcttcggttt
    11821 gtggctgata actcatcatc ttctcctgca tattcagttc tctgttctgg cgcttaacga
    11881 gccaaatccg gcaatccact ccgggattgg gcggtaataa cgcccttcgc tacgccagtt
    11941 gccaaatgca gaaatagcgg caaaagcggc gacccaggtt ttgatttcat gtgtcgactt
    12001 tccggcaatg gcggaaagct cttcgttact gacggcatcc agttcctgta tgcgtccctg
    12061 ctccagcaaa gtcatgaact ggttatccca aatcgggttg agcggatgca gagttctctg
    12121 atcctcaaca aacttctcag cggcgctaat cacccgttgc tgacgcaatt cgcgctcact
    12181 ggcgggtaaa tctttcccgc tccccaacag acggtcgcgc atatgggcat cggctttcgc
    12241 cagttcgggc accggcggct gatgggaaag cccaccggaa cccaggaaca gcacgcgttt
    12301 attgagagtg ctggtgaaac gtccaatggc ttcacccaac atgcgggtac gctggaaacc
    12361 gggcagcggc gtggcgacac cgttgatgaa cacaggcaga actggcacct tatccagccc
    12421 gccgagcagg aactccagtg gctgggcgaa cccgtggtcc acctgcatac agtaagaaac
    12481 ggcaagatcg atcccgctct tcatgacggc atgcgcacag gcctccgcca gctccacagg
    12541 cactggcagc tctcctgccg cactgccgaa atcaccaatt gccgtcgctc caacgcctaa
    12601 acagaacggt ggcatcacgt catagaaaaa gccgttgtag tgatctggcg caaacagcac
    12661 caccagttca ggggagaaag ccgcaatacg ctcgcgggcg ctggcaatca cgccattgac
    12721 ctcatcgagc acctcttgcg ccgggtcgac ataccccacc agcggcgagt gggaaagaca
    12781 gtgaagataa gcgtgcatat caggctacct tttcgacaga aacgtcggca tcagggcggg
    12841 tcagcgtcat caccgacgcc agtttattca gggtcttgcc cagggtttgc ggaatggcgg
    12901 tggcggcaac aaagcgatcc gggcgcatca ccaccagcga agcattgtgt tgcgcgaacc
    12961 agctacgcag acgaccttgc gtatcgccca cgcgtagcac gccgtcgtgg ttatcctgtg
    13021 cggtatgaat ttgcacttcc ggcaccacct gaatgaagcg tgtgcccaac gcgcgccact
    13081 gctggatttg ctcgtcgctc atcccccaca gtggattgca tccccagcca attaccgcga
    13141 agttcgcgcc aatcgcgtta tcgagcagcg tcacgtcacc gttttccagc gtgactttcg
    13201 gctgaataaa catcttgccg accggagagt gcttcgcttc gccctcacgc accagcgcac
    13261 cgccgtaata ttgcggcatc ggcttgaagc gcatttcgag gaaatagcgt tttactggcg
    13321 gcagataatt caacagccag gaaacgccgt cacgtaacgt accctgccag cgtttcggcg
    13381 gagccagcac gttgcccgcc gtcacggaca ggtcaatcat cgctttggcg tgatcgcgac
    13441 gttcttgttg ataggtatcg agcagcgcat cgcgggcttt cccctggata accaacgcca
    13501 gtttccaggc gaggttaaag gcgtcgcgca taccactgtt atagccctgc ccctgccaca
    13561 ccggcatgat gtgcgcggca tcgcccgcca gcagtacgcg atcaatacgg aaacgttgcg
    13621 ccagtcgcgc gttatgggtg tagacacgct ggcgaatcaa ttcaacattg tccggattag
    13681 gcagcacttt gcttaacagc ttgcgcatat tttgcggctc acgcagctgc tcttcggttt
    13741 ctcccggcat caccataaat tcaaagcgac gtaccgcatg aggcagcgcg gcagaaacat
    13801 acgggcgcac cggatcgcaa cacaaataga tatgcggcgt acttaacgga tcgttggcga
    13861 tatctaccac aatccactga tttggcgcag ttttaccttc aaacggcaca ttcagagtgc
    13921 gacggacaaa acttgctcca ccgtcacagg ctaccagcca ctgggctttg accgtttccc
    13981 gctgcccttc tgccgttttc aggtgcaagg tcacttcgtc attttgctgg ctgaaggcct
    14041 ccagctcgcg ggaaaacaag cagcgcacat tcggaaaacg cgacaaccct tccagcatca
    14101 ccgcatcgac ctgaggctga ataaaggcgt tacggcgcgg ccagccaaat tcatcggtca
    14161 ttggctgaat atcagcaaaa cagcggcctt tcggggtgag aaaacgcatc gcgtgccacg
    14221 gcgtagtgtg cggcagaaca tcatcgacca ggccgaccga ctgcatggtg cgcagcgcct
    14281 cgtcatcaat accaatcgca cgcgggtagt cgatcaactt atcgagtttt tccaccacca
    14341 gcacgtcaat gcccatctgg ccgagatagt tcgccatcat cagcccgacc ggaccggcac
    14401 cagcgatcgc cacctgaacg ctatggttaa cagcaggctg gatgtcaggg tgttgtattg
    14461 ccatttcagt acctcacgac tcggacaaaa tgccgttgcg cgcacagtac ggcgcaactt
    14521 attttgttaa aaacatgtaa atgatttttt attgtgcgct cagtatagga agggtgtttt
    14581 cggctacaat caaaacatgc ccgaatgtgc accaggtgca caactttgtt ttaactatag
    14641 aaatgtcaat taatatgcag aacaatgagc agacggaata caaaaccgtg cgcggcttaa
    14701 cccgcggtct aatgttatta aatatgttaa ataaacttga tggcggtgcc agcgtcgggc
    14761 tgctggcgga actcagcggc ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc
    14821 aggaagaggg atatgtccgc cgtagcccct ccgatgatag ttttcgtctg accatcaaag
    14881 tgcggcaatt aagcgaagga tttcgtgacg aacagtggat ttctgcactg gcggccccac
    14941 tgctgggcga tctgttgcgc gaagtggtat ggccgacaga tgtgtccacg ctggatgttg
    15001 atgcaatggt ggtacgcgaa accactcacc gtttcagccg cttatccttt caccggccaa
    15061 tggtcgggcg acgtttgccg cttctgaaaa ccgcctctgg cctgacctgg ctggcctttt
    15121 gcccggaaca agaccgcaag gaattaatcg aaatgttagc ctcccgcccc ggtgatgact
    15181 atcaactggc acgggaaccg ttaaagctgg aagccattct ggcgcgcgcg cgcaaagagg
    15241 gttacggaca gaactaccgc ggctgggatc aggaggagaa gatcgcctct atcgccgtac
    15301 cgctgcgcag tgaacaacgg gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa
    15361 tgaccattga acaggcagcg gaaaagcatc ttccggcgct acaacgggta gcaaaacaga
    15421 tcgaagaagg ggttgaatcg caggctattc tggtggccgg acggcgaagc ggcgtgcatt
    15481 tacgttgaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgtccggaa
    15541 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgc tatacgatgt cgcagagtat
    15601 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
    15661 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
    15721 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
    15781 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgctgatca actgggtgcc
    15841 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
    15901 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
    15961 gccactgctg tggaagcagc ctgcgctaat gttccggcgt tatttcttga tgtctctgac
    16021 cagactccca tcaacagtat tattttctcc catgaagacg gtacgcggct gggcgtggag
    16081 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
    16141 tcggcgcgtc tgcgtctggc gggctggcat aaatatctca cacgcaatca aattcagccg
    16201 atagcggaac gggagggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
    16261 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
    16321 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
    16381 tacgacgata ccgaagacag ctcgtgttat atcccgccgt taaccaccat caaacaggat
    16441 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
    16501 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
    16561 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    16621 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    16681 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg aaattgtgag
    16741 cggataacaa ttttacacag gatacagcta tgactatgat tacggattca ctggtcgtcg
    16801 tattacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    16861 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    16921 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
    16981 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
    17041 ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca
    17101 atccgccttt tgttcccgcg gagaatccga cgggttgtta ctcgctcaca tttaatattg
    17161 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggtgtt aactcggcgt
    17221 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagccgt ttgccgtctg
    17281 aatttgacct gagcacattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc
    17341 gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc
    17401 gtgacgtctc gttgctgcac aaaccgagca cacaaatcag cgatttccat gttgccactc
    17461 actttaatga tgatttcagc cgcgcggtac tggaagcaga agttcagatg tatggcgagc
    17521 tgcgcgatga gctgcgggtg acggtttctt tgtggcaggg tgaaacgcag gtcgccagcg
    17581 gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
    17641 cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc
    17701 gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gccagcgatg
    17761 tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
    17821 tgattcgcgg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
    17881 agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt
    17941 cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg
    18001 tagatgaagc caacattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc
    18061 cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc
    18121 acccgagtgt gatcatctgg tcgctgggaa atgagtcagg ccacggcgct aatcacgacg
    18181 cactctatcg ctggaacaaa tctgtcgatc catcccgccc ggtgcagtat gaaggcggcg
    18241 gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc
    18301 agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga
    18361 cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggcttcg
    18421 ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact
    18481 gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg
    18541 gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg
    18601 accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc
    18661 gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg
    18721 agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc
    18781 tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga
    18841 gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag
    18901 aagccggaca catcagcgcc tggcagcagt ggcgtctggc ggcaaacctc agcgtgacac
    18961 tcccctccgc gccccacgcc atcccgcaac tgaccaccag cgaaacggat ttttgcatcg
    19021 agctggataa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga
    19081 ttggcgatga aaaacaactg ctgaccccgc tgcgcgatca gttcatccgt gcaccgctgg
    19141 ataacgacat tggcgtaagt gaagcgaccc gtattgaccc gaacgcctgg gtcgaacgct
    19201 ggaaggcggc gggccattac caggccgaag cggcgttgtt gcagtgcacg gcagatacac
    19261 ttgccgacgc ggtgctgatt accactgtcc acgcgtggca gcatcagggg aaaaccttat
    19321 ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg
    19381 ttgaagtagc gagcgataca ccgcatccgg cgcggattgg cctgacctgc cagctggcgc
    19441 aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc
    19501 ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg
    19561 tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggctcacacc
    19621 agtggcgcgg cgacttccag ttcaatatca gtcgctacag ccaacaacaa ctgatggaaa
    19681 ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc
    19741 atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga
    19801 gcgccggtcg ctaccattac caattggtct ggtgtcaaaa ataataataa ccgggcaggc
    19861 catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact
    19921 tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt
    19981 ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt
    20041 ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca
    20101 aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg atgtttgcgc
    20161 cgttctttat ttttatcttc gggccactgt tacaatacaa cattttagta ggatcgattg
    20221 ttggtggtat ttatctaggc ttttgtttta acgccggtgc gccagcagta gaggcattta
    20281 ttgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg tttggctgtg
    20341 ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat aatcagtttg
    20401 ttttctggct gggttctggc tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa
    20461 cggatgcgcc ctcttccgcc acggttgcca atgcggtagg tgccaaccat tcggcattta
    20521 gccttaagct ggcgctggaa ctgttcagac agccaaaact gtggtttttg tcactgtatg
    20581 ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat ttctttactt
    20641 cgttctttgc taccggtgaa cagggtacgc gggtatttgg ctacgtaacg acaatgggcg
    20701 aattacttaa cgcctcgatt atgttctttg cgccactgat cattaatcgc atcggtggga
    20761 aaaacgccct gctgctggct ggcactatta tgtctgtacg tattattggc tcatcgttcg
    20821 ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa gtaccgttcc
    20881 tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt tcagcgacga
    20941 tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg tctgtactgg
    21001 cgggtaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg ggtctggtgg
    21061 cgctgggctt caccttaatt tccgtgttca cgcttagcgg ccccggcccg ctttctctac
    21121 tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc gcgagcgcct
    21181 tatccgacca acatatcagg acggaatgat cgcaatgaac atgtcgatga cagaaagaat
    21241 aaaagcaggc aagctattta ccgatatgtg cgaaggctta ccggaaaaaa gacttcgtgg
    21301 gaaaacgtta atgtatgagt ttaatcactc gcatccatca gaagttgaaa aaagagaaag
    21361 cctgattaaa gaaatgtttg ccacggtagg ggaaaacgcc tggatagaac cacctgtcta
    21421 tttctcttac ggttccaaca tccatatagg ccgcaatttt tatgcaaatt tcaatttaac
    21481 cattgtcgac gactacacgg taacgatcgg tgataacgta ttgattgcac ccaatgttac
    21541 tctttccgtt acgggacacc ctgtacacca tgaattgaga aaaaacggtg agatgtactc
    21601 ttttccgata acgattggca ataacgtctg gatcggaagt catgtggtta ttaatccagg
    21661 cgtcaccatc ggggataatt ctgttattgg cgcgggtagt gtcgtcataa aagacattcc
    21721 accaaacgtc gtggctgctg gcgttccttg ccgggttatt cgcgaaataa acgaccggga
    21781 taagcaatat tatttcagag attacaaagt tgaatcgtca gtttaaatta taaaaattgc
    21841 ctgatacgct tcgcttatca ggcctacaaa ttcagtgatc tacgtaggcc gcatccggcg
    21901 tgaacaaagc gcagaaacaa gagtcgcatc atgcctcttt gacccacagc tgcggaaaac
    21961 gtgctggtgc aaaacgcagg gttataatca tcagcccaac gacgcacagc gcatgaaatg
    22021 cccagtccat caggtaattg ccgctgatac tacgcagcac gccagaaaac cacggggcaa
    22081 gcccggcgat gataaaaccg attccctgca taaacgccac cagcttgcca gcaatagccg
    22141 gttgcacaga gtgatcgagc gccagcagca aacagagcgg aaacgcgccg cccagaccta
    22201 acccacacac catcgcccac aataccggca attgcagcgg caaccagata aagccgcaga
    22261 accccaccag ttgtaacacc agcgccagca ttaacagttt gcgccgatcc tgatggcgag
    22321 ccatagcagg catcagcaaa gctcctgcgg cttgcccaag cgtcatcaat gccagtaagg
    22381 aaccgctgta ctgcgcgctg gcaccaatct caatatagaa agcgggtaac caggcaatca
    22441 ggctggcgta accgccgtta atcagaccga agtaaacacc cagcgtccac gcgcggggag
    22501 tgaataccac gcgaaccgga gtggttgttg tcttgtggga agaggcgacc tcgcgggcgc
    22561 tttgccacca ccaggcaaag agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt
    22621 gataccagga ttcgctatgt tgaactaacc agggcgttat ggcggcacca agtccaccgc
    22681 cgcccatcag agccgcagac cacagcccca tcaccagtgg cgtgcgctgc tgaaaccgcc
    22741 gtttaatcac cgaaggcatc accgcctgaa tgatgccgat ccccacccca ccaagcagtg
    22801 cgctgctaag cagcagcgca ctttgggggt aaagctcacg catcaatgca ccgacggcaa
    22861 tcagcaacag actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc
    22921 cggccagcgc cagcccgccc atggtgacca ccggcagagc ggtcaacagg gcagccacgc
    22981 taaagctcat tccgctcgcc tggcgcaatt gcggtagcag tggcccgacg gaggtgagca
    23041 gtggtcgcat attaagacca atcagcacca gaaccagcag catcgtgaac gtcttttgat
    23101 gattgcgcat ggctgtcaga acggcttggt cggcagatat ttaccatcta aggtaatgac
    23161 cgcacgttcg ccaccttccg ggtccgccac tttcttaacg tcgagtttga agttaatcgc
    23221 gctgataatg ccatcgccaa acttctcatg aaccaacgct ttcagggttg tgccgtacac
    23281 ctgcaacatt tcatagaaac gatacatcgt tgggtcggtt ggaatacggt catcaatgca
    23341 gccacgcagt ggaatcatct gcaacagtag aatggcgtct tcgtcgagat ccagcttcgc
    23401 cccgaccagg cgggcggcgt cggcaggaag cgcttgctgc cccagcaaag ccgcggttac
    23461 gaaggcttct gccagaccgg taccgtcggc aatctcggca aatgagagat cttttttagc
    23521 tttgctgagc aaaatggcat cggcaagatc aagacgaata ttgcggttaa tttgtgactg
    23581 aatcatggtg gatctcctga tggtttaaaa ataaggttac gctgcggtcg gttggcgtag
    23641 cggtatggca caaacgcgag gattagcggc cagtggcaca aactggcggg ttgcgccgtc
    23701 aaaagctgcg atgctgccgc tttcaatgtc gtagacccag ccgtgcaggg cgatccgccc
    23761 ctcttcgagc gccaggcgca ccgatggatg agtttgcaaa ttagccaact gagcaatgac
    23821 gttttcacgt accatcgccg cagcttttga cggtaaatcg gaatgcgggc gcgcctcatt
    23881 aacgacgcgg gctgaatcgg cataacgcag ccagtgggag acggcaggca tatggtccat
    23941 gcactgacag ctggcaatgg cggtcatcgc gccacagttg gaatgaccac aaatcacaat
    24001 gtcagatacc cgaagcgcag cgacggcata ctccaccgaa gcagaaacgc caccgggttc
    24061 cggcccgtag gaagggacga tattgcccgc gttgcgaata acgaacagat cgccaggctc
    24121 acgttgcgtc accagctcag ggaccagacg gctgtcggag caggagataa aaagtgtgcg
    24181 cgggctttgc tgtgtcgcca gctgtttaaa caaggcttcc cgcttcggaa atgcctcgcg
    24241 ctggaattta aggaatccat caataatctc tttcaccgtt aacctctgtc tgtctctgga
    24301 atgagaggca gactactcgc cgattgtcat aaggtaaaag tctcatttat gatgagttct
    24361 attggattta cttataggtt ggcgaatgct ctctcgacat atcaattact ttcttgccgt
    24421 ggctgaacat ggcagcttca cccgtgccgc cagtgcgttg cacgtctccc agcctgcgct
    24481 ttcccagcag attcgccagt tagaggagag tttgggcgtg ccgctgtttg accgtagcgg
    24541 gcgaacgatt cgtctcaccg atgcaggaga agtctggcga cagtacgcca gccgggcgtt
    24601 acatgaactg ggagcgggta aacgggcgat tcatgatgtt gccgctctga cgcgaggatc
    24661 gctgcgtatc gccgtcaccc ccacctttac gagctacttt atcggcccct taatggcgga
    24721 tttctatgcg cgctaccccg gcatcacgct ccagctacag gaaatgtcgc aggagaaaat
    24781 cgaagatctg ctttgtcgcg acgagctgga cgttgggatt gccttcgcgc ctgtgcattc
    24841 gccggagctg gaggcaattc ctttactgac agaaagttta gcgttggtgg tggcgaaaca
    24901 tcatccgctg gctgcctgtg aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt
    24961 cctgctcagc gcggaatttg ccaccagaga gcaaattgac cactactgcg agaaagcggg
    25021 gctacatcca caggtggtca ttgaggcgaa ctcaattagc gcggttctgg agctgattcg
    25081 ccgcacttcc ctttccacat tgttaccagc agcgattgcc acacaacatg acgggcttaa
    25141 agcgatttct cttgccccgc cactactgga gagaacggcg gttttgttgc ggcggaaaaa
    25201 tagctggcag actgccgctg cgaaggcatt tttgcacatg gcgttggagg aatgtgcgga
    25261 tgttggcgag aatgaatcac ggtagccact gacacaggtc agtggatctt caacacagag
    25321 gaaattaccg tttgtaatcg atggcttctg gctgctccag atatacggtg gtttgtgccg
    25381 gttgtgtact ggcaatcacc ttgccgccac gtaccgaata acgtaccgga acctgacggc
    25441 gcagcgcatc aaatccattt tcagccggca ggataatcag gtttgcgctg tttccggcgg
    25501 caatgccgta atcctgcaaa ttcaatgttc tggcactgtg gtgggtgatt aaattcaggc
    25561 catcgttaat ctgcccgtag cccatcaact ggcaaacatg cagccccata tgcagcactt
    25621 gcagcatatt cgccgttccc agcggatacc acggatcgaa gacatcatcg tgaccaaagc
    25681 agacgttaat gccggactcc agcatctctt taacgcgcgt gatgccgcga cgttttggat
    25741 acgtatcgaa acgtccttgc agatgaatat tgaccagcgg gttggcgaca aagttaatac
    25801 cggacatttt cagcaagcgg aacaggcgtg aggtatacgc cccgttatag gagtgcattg
    25861 ccgtggtgtg gctggcggtg actcgcgcgc ccatgccttc acggtgcgcc agggcagcaa
    25921 cggtttcgac aaagcgcgac tgctcgtcat cgatctcatc acagtgaacg tcgatgagac
    25981 ggtcgtattt ttgcgccagg gcgaaggttt tatgcagcga ctccacgccg tattcacggg
    26041 taaattcaaa atgcggaatc gcccccacta catctgcccc taagcgtaac gcctcttcca
    26101 gcaacgcttc accgttggga tacgacaaaa tcccttcctg agggaaggcg acaatttgca
    26161 gatcaatcca cggcgcgact tcctgcttca cttccagcat tgctttcagc gcagttagcg
    26221 ttgcatccga aacatcgaca tgggtacgca catgctgaat gccgttggca atctgccatt
    26281 tcagcgtttg ccaggcgcgt tgtttcacat cgtcatgggt taataacgct ttgcgctcgg
    26341 cccagcgttc aatgccttca aacagcgtgc cggactgatt ccagttcggt tgtccggcgg
    26401 tttgcgtggt gtccaggtga atatgcggct ccacaaacgg cggtataact aaaccttgtt
    26461 cggcatccag gctgttttca gttatgggca tcacgccgga ttgcgcatca atggcgctga
    26521 tttttccgtc ctgcagatga atctgccaca gcccctcttt gcctggtaac cgggcgttaa
    26581 taattgtttg taaagcgtta ttcgacactg ttaacctcca catgcgtcat tgctgctgtc
    26641 gttttacgat tcaaaatcgg gttaaggatc agatagctca gcgcgccacc taataccgcg
    26701 ttgaccggaa caattcccgg taaccagtgg cctgcggcaa tccccagcgc gaccgccaga
    26761 atcgccaccc aattgacact catcatacgc gtggtcgcaa agtgctcata gcggcgacgg
    26821 ttcatcagat agtcggcgat gatcacgccc cctactggag gaatagctgc cgaaaggaag
    26881 gtcaaccagc cgacaaaatt gttatacagc cataatgcgc agaccgtacc gataataccg
    26941 ttgattaccg aaagggtttt gctcgacata ccggtaatgt tggcgaaacc taaacccgac
    27001 gcatagagtg cgttatcgtt ggtggtccag atattcagcc ccagcaccac aatcgcaggc
    27061 agcagcaggc cctgagcaat catcacatca gagatatccg ccatgcccag tgccgcagcg
    27121 cccgctgcac cgaaaataaa catcaacgag ttgccgagga aaaaggccac catcgccacc
    27181 agcaccgcca gtttggcatt gcgaccaaac cggacaaagt cagcggtgag cgtacccgca
    27241 ctgataaatg accccacaac cagcgccagc gcgacattga aatctaacgg ttgtgcggga
    27301 acgaccgctt ttaatgcgtc caggccgccc atgccgttaa cagccagcca cacggaataa
    27361 ccgcccaggc aggcgatagc cggaaccgca atcaccgaaa gaaccgtcag cgccgaaatg
    27421 ccaaaaaaga cggtgacggt catcagtaaa ccggaaacgg caatcagcaa attaatatcc
    27481 agcccggttg ccttacccac cggaatggca aacatcgcca caccgacgcc aaaccagcca
    27541 acctgagttc cgcccagtag cagtgaaggc agccatgagc ctttaacacc aaacgagaag
    27601 cgagcaagaa gatgagtggt caggccggtt tttgcgccaa tgtaaccgag aaatgaagtg
    27661 taaataccga ggagaagatt accgatgaga actgcgagga agaaatcatg atagctaaga
    27721 ccggttccga gagtgccgcc ggtccacata ctggcggaaa agaaggttaa tcccagcatg
    27781 acgaacgtca atgccaatac ccctttccgc gccgactgcg ggactggccc ctggctaaag
    27841 ttgttatctt gcgacacgaa attccccctt tttgctgttt gaaacccaaa aaatccgccg
    27901 cattctattc atctgaagat aaaaagcaat cgttttcgtg gggaaatata ttttttatat
    27961 gaatgagtgc aaaaaggtgg gggaaaaatt atcggtgtaa aaacagtggg tagtgggtag
    28021 gcctgataag acgcgtcagc gtcgcatcag gcatcggcgc atggtgtcgg atgcgccacg
    28081 cgacgcatcc gacaatacct tactcttcca tcgcctggcg gatctgatcc aatgacgcag
    28141 gatcatcaat ggtcgtcaga tccccaggat cgcgtccttc gcaaattgcc tggatcgtgc
    28201 ggcgcagcat ttttccggat cgcgttttcg gcaattgcga gacaaaccag acgtgcgccg
    28261 ggcgaccaaa gttgccaatc tggctgtcca ccagcgccat aatcgccttc tcttgcgaat
    28321 gcgccacatc acgatcttcc agactatcgc tctctttcgg aatgacaaac gccaccgcca
    28381 cctgcccttt cagcgcatct ttcaccccaa ccaccgccac ttcggcaacg cccggatggc
    28441 tggagatact ctcttcaatc tcgcgcgtgc ccagacgatg cccggcaacg ttaattacat
    28501 cgtcagtgcg cccgagaata aagtgataac cgtcagcgtc acggatgccc cagtcaaagg
    28561 tggcgtacac cgggcgggaa aacagcgacc agtaagtctt cacaaagcgg tcgtcgtcgc
    28621 cccagatggt ctgaatacag cccggcggca gcggcccttc caccaccagc atccctttct
    28681 cgttgacgcc acacggttcg ccggtgactt cattaagcaa ctgcacgtta tagccataca
    28741 tcggcacgcc ggggcttccc agacgcgtcg gcctgtcgtc cagaccgcga gcaatcgcca
    28801 taatcggcca gccggattcg gtctgccagt agttgtcgat gaccggcaca tccagcgtat
    28861 tgctcaccca actggcggtc ggctcgtcca gcggttctcc agccagatag agcacttcca
    28921 gcgacgagag atcgtgtttg cgaatttcag cggtagggaa ttttttcagc acgcgaatgg
    28981 cggtcggcgc tgagaacatc cggctaacct gatatttctc gacgattttc caccacacgc
    29041 cgcagtccgg ccaggtcggc aatccttcgt aaacgatagt cgccatcccc gccagcagcg
    29101 gcgcgtaaac gatatacgaa tgccccacca cccagccgat atccgatgcg caaaagaaca
    29161 cgctgcccgc tttgccgcca aaaatggtgt ccatcgaggt cgccagcgcc accgcatatc
    29221 cgccgacgtc acgctgcacg cctttaggtt tgccggtcgt gccggaagtg tagagaatgc
    29281 aggaggtttc gttggattcc agccacgcca ccggtacccg cgcgccgatg tgttgatggc
    29341 gcaacgacgc gaaatcgaca tcccgcccgc tgacgcgcgc cattttcgcc agcccgcgat
    29401 ccaccagcaa aacatggcgt ggctggtgct gcgcctgact tatcgcatcg tcgagcaatt
    29461 ttttataggg aatgattttg ccaccgcgcg ctccggcatc agccgagaca atcagcaccg
    29521 gtttagcgtc atcaattcgc gccgccacgc tgtgcgaggc aaaaccacca aataccaccg
    29581 aatgaatagc accaatgcgc gcgcaggcca gcagagtaat atgcgcttcg gcaatcatcg
    29641 gcatatacac cagcacccga tcgccgcgct gcacgcccag tgagcgcaac attgaggcca
    29701 ccgcgttcac ttcgtcatgc agctggcgaa aggtaaaggt gcgctcttct tctgtttccg
    29761 aagagacggc aatcaatgcc agtgcctctg gctgtttctc cagccagcgg tcgatagcgt
    29821 tgtggcacaa attggttcgg ccttcacaaa accaacgggc aaacggcgga ttgctgtggt
    29881 cgagcgtttg cgtaaagggc gtctgccagt caatacgccg ggcctgctcg gcccagaact
    29941 gctccggttc gttaatcgaa cgctgataaa attcgctaaa agacataatg ctctcctgtt
    30001 gaacttacgc cttaccgccg tttacttaaa tgacgtacag gtcgagatac tcattgaccg
    30061 gcatctgttc caggcgagtt ctgtcgagag aaacctccag aatgcgctgc tgttggcgag
    30121 tcgggaactg gcgcgcgaga ttgattttga atttatcgac cagtttcgga ataccatcct
    30181 gacggcggcg agcatgacca atggggtact ccaccaccac ttcttcaaat cgtgtgccgt
    30241 cggtgaactc aagggtaatg gcattggcga tggcgcgttt ttccgggtcg tggtagtcag
    30301 cggtaaatgc cggatcttca aagcaattga tcttctcgcg cagggcgtcg atgcgtttat
    30361 cttgcgcaac gttgtcctcg taatctgccg ccgttaagcg cccgaataac agcgggatcg
    30421 ccaccatgta ctgaatgcag tggtcgcggt cagccgggtt attgagcggc ccctttttgt
    30481 cgatgatgcg aatacaggct tcgtgggtgc gaatggtcac tttttcgata tccgccgccg
    30541 tcttgcctgc tgcctgcatc tgttcataga gcgtcatcgc cgcttcaact gccgtctggg
    30601 agtggaactc cgccgggaag gagattttga acagcacatt ctccatgacg taagaaccgt
    30661 acggacgctg gaagcggaac gattcacctt taaaggagac gtcgtagaag ccccataccg
    30721 gcgcagtcag ggctgacggg taacccattt cgcccgtttt cgccatcagt gccagacgta
    30781 ccgcgcggga agtggcatcg cccgccgccc aggatttacg cgtgccggtg ttcggcgcat
    30841 ggcgataggt gcgcagcgac tgaccgtcca cccacgccag cgaaacggcg ttgagaattt
    30901 cctcgcgggt caggccgagc atttcggcga ccacggcggt ggaagccact ttcactaaca
    30961 gaacgtggtc gaggccgacg cggttaaagg agttttccag cgcgatgcag ccctgaattt
    31021 catgggcttt gatcattgcg gtcagcacct gtttcatggt caacggcgct ttgccgctgg
    31081 cgaccgcgtt gcgcgaaagc cagtccgccg ttgccagaat gccgccgagg ttgtcggaag
    31141 gatggcccca ctccgccgcc agccaggtat cgttgaaatc gagccagcgg atcatcgcgc
    31201 cgatgttaaa tgccgcctgg acggggtcga gctggaactg agttccgggg acgcgcacgc
    31261 cgttgggtac gacggtgccg ggaacaattg gccccagcag ttttttacag gccgggtatt
    31321 cgagagcttc cagaccgcag ccgagcgtgt cgagcaggca gtaatgtgcg gtgtcgtagg
    31381 ccactttaga gctgatttcg tagttcatga cgtaatcgac gatatcaacg atttcacgat
    31441 caaattccgg gcggatgttg ttgatttgag ctgacattgg gtacgtttcc ttattgttat
    31501 tcgtagaggt ttactggcgc ttatccagcg cgacaaacgg gcggtcttcc ggtccaacat
    31561 aattggcgga aggacggata attttgttgt cctgacgttg ttcgataatg tgcgccgccc
    31621 agccggtgac gcgggcgata acaaacagtg gtgtgaacat ctcggtggga acgcccatca
    31681 tgttgtagga aacagcagag aaccagtcga gattggggaa catctttttg ctctcccaca
    31741 tcaccgtttc caggcgatcg gcgatgttgt acatcttcag cgagccgcct tcctgcgaga
    31801 gctgcttcgc cacacgttta atcacctggt ggcgcgggtc agcgatggtg taaaccggat
    31861 gaccaaaacc aatgaccact tctttgtttt ccacgcgctt gcggatatct gcttcggctt
    31921 cgtccggcgt ttcgtagcgt tgctggatct ccagcgacac ttcattcgcc ccgccgtgtt
    31981 ttggcccgcg cagtgcgcca atcgcgccaa taatcgcgga atacatatca gagccggtgc
    32041 ccgcaatcac ccgactggta aaggtggagg cgttaaactc gtgttcggcg tacagcacca
    32101 gcgagatatg catcgccttt tcccagcttt gcgatggctt ttcgccgtgc agcagatgca
    32161 ggaagtgacc gccgatggag tcgtcatcgg tttccggctg gatgcgttcg ccgttgtgac
    32221 tgtagtgata ccaataaagg agaatcgagc taagtgatgc cagcagtttg tcggcaatat
    32281 cccgcgcgcc agagacagtg tgcccctctt tttctggcag tgtgcagccg agcgcggaaa
    32341 cgccggtgcg catgacatcc atcgggtgcg acgccgccgg taaggcttcc agcacggtac
    32401 gcacattagc tggtaaacca cgcagggctt tcagtttcgt tttgtaagcg gcgagttcgt
    32461 cacgggtcgg cagtttgccg tggatcagca ggtgcgccac ttcttcaaat tcgcaatgtt
    32521 ccgccagatc aagaatatcg tagccacggt aatgcaggtc attgccactt ttacccacgg
    32581 tgcagagcgc cgtattgccc gccggaacgc cagaaagtgc taccgatttt ttcggtttaa
    32641 tgacatgggt actgttttgc aggatcgttg tgtcgctcat attgtcctcg tcatttgtag
    32701 ggtcgagata tttttcgttg tgttttattt cacctggcta cgggcaaaca ggtcgtcgag
    32761 cttctcttcg tactggtagt agttgatgct ttcgtacagc tcgttgcggg tttgcatggt
    32821 atcgatgacg cttttctgcg tgccttcctg acgcaggacg ttgtagacat gttcagcggc
    32881 gcggttcatg gcgcggaacg ctgaaagcgg gtacagcgcc attgcgacat gggcgctgcg
    32941 taattcgtcg gtggtaaaca gcggtgttgc gccaaattcg gtaatgttgg cgaggattgg
    33001 cacctgcacc gcatcggcaa actggcggta catggcgagt tcggtaatcg cctccgggaa
    33061 caacatctcg gcacccgctt caacataggc ctgcgcacgc tcgatcgccg catccagccc
    33121 ctctaccgcc agagcatcgg tgcgcgccat gatcacaaaa tcaggatcgg ttttcgcatc
    33181 caccgccgcg cggatccgat ccaccatctc ttctttcgag acgatcgctt tattcggacg
    33241 atgaccgcag cgtttcgcac caacctgatc ttcaatatgc aatcctgccg caccggcttt
    33301 aatcatcgat ttcacggtgc gcgccacgtt aaaggccgaa gaaccaaaac cgatatccgc
    33361 atccaccagc agcggcagcg aacaaacgtc ggtgatacgg cgaatgtcgg tcagcacatc
    33421 atcaagggta gaaataccga gatcgggcag ccccagcgaa cctgccgcca cgccaccgcc
    33481 agaaagataa attgcctgat atccggcacg ctgcgccaac agcgcatgat tagcgttgat
    33541 ggtgccaaca atctgcaatg gattttcttt agtcagtgca gcgcgaaacg ctttacctgg
    33601 agagtgtaga gacatagccc atcctttgtt atcaacttgt tatttgcgtt gataaagaca
    33661 aagcaagggg tgtgccacaa ttgccttaat aaaattaaat gtttaatttc aatttgttag
    33721 agataaagtc atgaactaat ccgcaaatat gcgtttcagt taacgtttca ggcaatgttt
    33781 caccgcgttt cattgcaaca attatgaaac aagactaaac ccaatattcg gtttcttaac
    33841 tttgcggtgc gctatggcac atccaccacg gcttaatgac gacaaaccgg ttatctggac
    33901 ggtatctgta acgcgcctgt tcgagctgtt tcgcgatatc agcctcgagt ttgatcacct
    33961 ggcgaacatt acccctattc agcttggctt tgaaaaagca gtgacctaca tccgcaagaa
    34021 actggcaaac gaacgctgtg acgccatcat cgcggctggc tctaacggcg cgtacctgaa
    34081 aagccgcctg tcagtgccag ttattttgat taaaccgagc ggctacgatg tgttacaggc
    34141 actggcaaaa gccggaaaac tcacctcttc tatcggcgtt gtcacctatc aggaaaccat
    34201 tccagcgctg gtggcgtttc aaaaaacctt taatttgcgc ctcgatcaac gtagctacat
    34261 taccgaagaa gacgcacgcg ggcagattaa cgagctaaaa gctaacggca ccgaagcggt
    34321 ggtcggcgcg gggctgatta ccgatctggc agaagaagcc ggaatgaccg gaatttttat
    34381 ctattccgcc gccaccgtgc gccaggcgtt cagcgatgcg ctggatatga cgcgcatgtc
    34441 gttacgccat aacactcacg atgccacccg caacgccctg agaactcgtt acgtgctggg
    34501 cgatatgctc ggtcaatcac cacagatgga acaagtacgg cagactattt tgctgtatgc
    34561 ccgctccagt gcagcggtgt tgattgaggg ggaaacgggg acgggcaaag agctggcggc
    34621 ccaggcgatt catcgggaat attttgcccg ccacgatgcg cgacagggca aaaagtcgca
    34681 tccgtttgtt gcagtcaact gcggggcgat tgccgaatcg ctgctggaag cagaactgtt
    34741 tggctatgag gaaggggcgt ttaccggctc gcgacgcggc ggtcgcgccg ggctgtttga
    34801 aatcgcccac ggaggtacgc tgtttctcga tgagattggc gaaatgccgc tgccgttgca
    34861 gacccggctg ctgcgggtgc tggaagaaaa agaggtcacc cgcgtcggcg ggcatcagcc
    34921 tgttccggtg gatgtgcggg tcattagcgc cactcactgc aatctggaag aagatatgcg
    34981 gcaagggcag tttcgccgtg acctgtttta tcggctgagt attttgcgtc tgcaattgcc
    35041 accactgcgc gagcgggtgg cggatattct gccactggcg gaaagctttt tgaaagtgtc
    35101 tctggcggcg ctctccgccc cgttttctgc cgcattacgc cagggattac aggcaagcga
    35161 aaccgtgctg gtgcactacg actggccggg caatattcgt gaactgcgca atatgatgga
    35221 gcgactggcg ctatttttaa gtgtggaacc gacgccggat ttaacgccgc aatttttgca
    35281 gctgctactg ccggaactgg cgcgcgagtc ggcgaaaact cccgctccac gcttactgac
    35341 accacaacag gcactggaga aatttaatgg cgataaaaca gcagcggcga attatttagg
    35401 catcagccgg acgacgttct ggcggcggct gaaaagctga actgccgaat gcagcgcgaa
    35461 cgctttatcc ggcataaatt tcaatagatg atcaccgata aacttacgca tcgggcatgt
    35521 ttacataggg ttcagaatta cttcttctta taaatatttg ccgtgccgtg gatcttattg
    35581 tcagtttgac cggaggtcag taccaacaca tcagcccctt tttcatccgc ttttttgatc
    35641 aaatcttctt tcgcgtctgc agtcgacatt tcattgctgg ttgaaatatc acctattttt
    35701 tcatactgcg attcaacttt ttcaaattcc gctttggtca tcaattcagc ggcatagaca
    35761 ttggtaacgg ctaacgctaa cgcaccgact aacattttag agattatttt catattacgg
    35821 ctccagagtt gttatcacac tatcacccac ccacattatt gggttgatta ataattagtt
    35881 cagtcagggc ataaacgcca gtaaccggaa ataagacact ttgtgatctc tctcgcaccc
    35941 gattattttt ggtagaaata tgatgaagtt acttcactta tttatggatg aaatcactat
    36001 ggatcctttg cacgccgttt acctgaccgt aggactgttc gtgattactt tttttaatcc
    36061 gggagccaat ctctttgtgg tagtacaaac cagcctggct tccggtcgac gcgcaggggt
    36121 gctgaccggg ctgggcgtgg cgctgggcga tgcattttat tccgggttgg gtttgtttgg
    36181 tcttgcaacg ctaattacgc agtgtgagga gattttttcg cttatcagaa tcgtcggcgg
    36241 cgcttatctc ttatggtttg cgtggtgcag catgcgccgc cagtcaacac cgcaaatgag
    36301 cacactacaa caaccgatta gcgccccctg gtatgtcttt tttcgccgtg gattaattac
    36361 cgatctctct aacccacaaa ccgttttatt ttttattagt attttctcag taacattaaa
    36421 tgccgaaaca ccaacatggg cacgtttaat ggcctgggcg gggattgtgc tcgcatcagt
    36481 tatctggcga gtctttctta gtcaggcgtt ttctttgccc gctgtgcgtc gtgcttatgg
    36541 gcgtatgcaa cgcgttgcca gtcgggttat tggtgcaatt attggtgtat tcgcgctacg
    36601 cctgatttac gaaggggtga cgcagcggtg agcagataat ttactctcta cgtaatcaac
    36661 ctgatttgcc gaatcccggc catcggcgat gatagccccc gtaatgcgtg gtccttattt
    36721 ccgataagtt tacttgagtc tttagcttcc catttcagcc cactctcccc tgtgtgggct
    36781 tttttcattg gtctgacccg tcctgaatag ccaaacaatg gcaatgttcc actttgcagg
    36841 gatgataata aggggatctg attcagaaga gttttgaaaa gttggaccgc catcgaggcc
    36901 tcgaaccccg taccatacaa caatcctggt tgtcgtctgc tcttcctgat gagctaatgg
    36961 cggtttttga tatgagcctt acagcatatt ttctgtcagg cttgagtgcg atgcgtaaga
    37021 tacagaaaag accccacaca aacatgatgg aattatgatt tttttcatat gaatgtcaac
    37081 ccacacattc acaaaacatc tgcatgagat cattttttat acaccgcatg gctgcgaaac
    37141 cactactggt gcttttttaa gcgaagtgtc gccgatcaca acaaaatatc ggcgaaaaaa
    37201 gagatggaat gccagttggc atacagatat acagatgaaa tgattacctg cattgcgatt
    37261 cactctcagg tggaataaca acttgcaggt ggaacccttt ctgaactaaa gagtgtagcg
    37321 gattttaacg tgcttattta caggataata tctgtcacct aattcctgga ctctattaga
    37381 tatccattga tttaatcgcg catcatctct gtatgttttt acgtccagaa cagagagcaa
    37441 taacatcacc agtgattcag gaaaaacagg gtagatattt tttaagtgta ataataatcc
    37501 atgcgcaagc gcctgcttaa tgggcgcgtc tgtctccgga atagacgaca tttcgcgaag
    37561 ccatttttcc acatgcatac actctttccc tttagggaaa attctcagca aaggcatatg
    37621 gtgttcaaga agaatacatt tgcaatctgt tcgcactgaa tcgataaact gcactaattt
    37681 cttaagcccg gtaaagcgtc cagcctgcac gtttgcttta atatcaagaa acgcatcatc
    37741 ctggtcatat acccaaactg tccctttttg attttcaagt atgcgaaaaa caaacttaac
    37801 cgggtgatca gggtaatcca tccagtcata aaatccaggg aaatcattaa actgcacatt
    37861 tatggtgagt gcgttatcgt ctgaccactc acagtctcgt acggcagcca tgagatgaat
    37921 gagtttgtcg tcatcgacta aatcatcacg acaatcataa ggataatcat aatattgttt
    37981 aatcaaaacc ttgaatatat cgacacagga aaccagggag cttttgatat cctctggtaa
    38041 ctgattgttt ttaaccgtat taagtatgct ttgttgcgta tatggcatca aattattgtg
    38101 cggagctttt aatgatatca tcgcgatctc tcttaaataa aaataaacgt cgcagataat
    38161 aaagctctga ttaaaccagg cactatcaga aatcgctcat aactaacgtg tgaagtattg
    38221 tgtactggag ggagttaatt taataatttg aatttacgtt ataggtttta ctgctacttt
    38281 gatattattt cgctctcctt aactaaaaag ttaagtggag agcgaagtta atatgttaaa
    38341 ccacagcgta tttattaatt ttttcagtct gttagtgtgc gattatcgat aacaaaacga
    38401 tatttcacat caccgcgcag cattcgctca taggcttcat taatttgatc ggcccgaatc
    38461 atctctatat cagccacaat gccatgttcg gcgcaaaaat cgagcatctc ctgagtttct
    38521 ggaatgccgc caatcataga accggctatc gcacggcgtt tcatgatcag gttgaaaact
    38581 tccggcgatt tatgcggtgt cgcaggcgca ccaaccagcg tcatggtgcc atcacgcttc
    38641 agcaaggtgg taaaatcgtc gagattatgt ggcgcagcta ctgtattcaa aatgaaatcg
    38701 aaactcttca gatgagccgc catctcatcg gcattgcgtg agttaacaac ttcatcggcc
    38761 cccagggctt ttgccgcttc gcgttttgcc tcagaagtgg taaatgccac cacatgtgcc
    38821 cccatcgcgt gggccagctt aatccccata tgtcccagac cgccgatgcc gaccacgccc
    38881 acttttttac ccggcccggc ctgccagtga cgtagcggcg aatacgtggt gatccctgca
    38941 cacaacaaag gagccaccgc cgccagctgc tcttgcgggt gacgaatacg cagaacatat
    39001 cgctcatgaa cgacgatctg ttgtgagtag ccgcccagag tatggcccgg ttcgtccggc
    39061 gtcggcgagt tataggtgcc ggtcatgtga tcacagtagt tttccaaccc gtcttcacac
    39121 tcttcgcaat gtttacaact gtcgacaatg cagccgacac cgaccagatc gcccggcgca
    39181 tatttttcta cctgatcacc aacggctacc acacgcccca caatttcatg acccggcacg
    39241 caggggtaaa ccgtccccgc ccactcggaa cggacctggt ggagatcgga atggcaaacg
    39301 ccacagtaag cgatttcgat tttgacatca ttcggtcccg gttcacgccg ggtgatatcc
    39361 atcggttcaa gtggttgttt agcggaatat gcaccaacag ctttgatctt cattgtgttt
    39421 actcctgatt agctatgtgt atttggcgac gcctgatatg tcatcgcggc aaaacgcgtc
    39481 cattgaatat agccaatatt taaaccaccc gatccagccc caacatgccg cgagcctgaa
    39541 tgaaacagag cagcaacgtt ccccataagg ccatcgtcag gtaggggctt ataccgagaa
    39601 tattgaagcc gctttcaata atttgcagaa gaaacagcgc cagcaccata ccaattatcc
    39661 gcccgctgcc gccgtccgga ttaaccccgc cgagtactgc cgcgaggatt gacaccagca
    39721 agtacgattc gccataagag gccttagccg agttgagttt cgacatcatc aaaaatgccg
    39781 ctaccgcaca aagcaaggcg gaaatgacat aaacccaaat cagcacccga cgagtgttaa
    39841 tgccggagta aagtgttgct cgttcgttag acccaatcag gtaaatggct ttaccgagcg
    39901 gcgttttctc cagcaatatc catagcccca gtgccactgc ggcaaacaac cacatcggta
    39961 gcggaatacc gaaccattgc gcatgattca accacagcac ccatgaggga taattggcaa
    40021 tggcgctacc acctgtcacc agaatattta tccctttgag caacgtcatc atcccaagcg
    40081 ttgcaagaat tggcgacact cgaataccag caatcaatac gccgttacat aaaccgatta
    40141 tcaccgccgc gccagcgccc gccagtaacg tagcgacaac cgtagcaata cccggtggat
    40201 actgagtcgc cacccacgcc atcaccagtg aacaggcgtt agcagtggct ataattgaca
    40261 agttaatgcc accgcaaagc atggtcaccg ccatagccaa cgccagaatg cccagcacgg
    40321 gcatttgcga agcgacggac tggaaattac ttatcgacca gaaaatctcc ggcatagtaa
    40381 tgctgaacgc ggcaacagtt agcgccagca ggccgatcaa gtaaaactca acgttattac
    40441 gccacgattt tttcatagca gactccggtt ctgatgctga ctccacgccg tggcactaat
    40501 gctggcaacg atgatgatgc cggtgatcaa tgtttgccag taagacgaga ctcccaataa
    40561 atttagcccg ttttgcatca ctgccaacaa caccacgccg agcaaagtac cggttaacgt
    40621 gccgcgcccg ccgagcaaac tagtgccgcc aagcaccacc gcagccagta ctgtcagctc
    40681 ataacccaga agagaatcgg gggcgacagt catcaccgtc cacgactgca ctacacccgc
    40741 agcgccagac atcaatccca tatagccata gacgaaaagt tgcagtttca gcacgctaaa
    40801 gccgatgcga ctggctgatt cgcggttccc gccaagggca taaattttgc gccctacact
    40861 ggtgaaattc atgataaacg ctgttagcag caccaccgta atcatggcga tcagcggcag
    40921 accaaggcca tagtcatagc catcagcatc ggtgtacttg aacaacataa ccccctgctc
    40981 aaaccacggc ggaaagtcgt acagccacac acctttactc aaccataaca gcaggccata
    41041 gaaaatattc agcgtcgaga tagtgatgat gatagaaggc acccgtaggc gattaaccag
    41101 taaggcgtta attaagccaa gtaaaatgcc gatgcagccc gccagtgcaa acgcgacagc
    41161 gaagttgcca ccaatttttt gcagcaacag caccatgccg tattgcgcaa tgatggtcat
    41221 tgctggaaac gaaatatcaa ttccaccgga aatcaacacc acaaacaaac cacaggcgag
    41281 aatagtcaac atggcatagt tattggcgag atcgtacaaa ttaccgaatg tcagaaactc
    41341 gtcgctgcgc caggcaaggc caacaaacag caccacaatc agtaatgcca gccagaattc
    41401 atggcgtttt ttcagttcag ccatttacca cctccgcaat cctggcctga gaactgctgt
    41461 caggcaggaa gctatgggtg atctgacctt tttgcattac caaaatccgg tggctttgat
    41521 accacgcttc ttcaatttca tcgcagatca tcaataccgc aatgccatga gccgccagat
    41581 cgctgataat gccgtatatt cctgctttgt tggcgatatc aacaccgact gtcgggctat
    41641 cgagaatcaa cagtcgtggc ccaatcgcca gccatttggc gatcgatact cgctgcgcgt
    41701 taccaccaga aagcgtattc accggaagat gaggatcaga gactttgatg gtcaatgcct
    41761 tgattagcct gtccaccagg tcacgaactt tagtttcact cagaaagccg aagcgatctt
    41821 ttaccttgtg aaaaacagtg ctaatgatgt tgtcttcaat agattgcgcc atcaccaaac
    41881 cgcgcgacat gcgatcttcc gaaacataac caataccgtg gcggatagcg tcctggttac
    41941 tatgcagtgt aaccggctgg ccgttgatga ggatctcgcc agcatccggt cgggtcatac
    42001 caaacagact cagacataat tccgtgcgcc ccgcaccaag caggccaaca atcgatacca
    42061 cttcgcccgc ttccacccgc aagttaatat ttagatattc cccatgacga cttaaattac
    42121 gcacctccag caccggggtg ctggattttc cctgccacaa ctctcgcacc tgatattcaa
    42181 actcctggcc ggtcatcagg aaaccgagtt gttttgtggt catctctgca gccggaaaag
    42241 tcccgaccag ctcaccatct ttcagcactg aaatacggtc ggaaacttcc atcacttctt
    42301 ccagacgatg actgacgaag accacgcaga tcccgcgttc acgcaattga tgcaccacct
    42361 gtaataaccc ttgcacttcc tgatgggtca gcgaagcggt tggttcatcc atcacgatca
    42421 accgtgcgtc ctgcgccagt gcacggcaaa tagccaccag ctgacagcgg gcaattgaaa
    42481 gctcagaaac gagtgtatcc agtggcaatg tgacgttaat actggtcatt gccgcctgcg
    42541 ccacctcacg cagacgacgg cggttaacaa acagaccgtg gtgatagtga ttcacggcga
    42601 tattctccca gacactcagg ttaggaaata aagagaggtc ctgataaatc acctgaatcc
    42661 cctgcgccac cgaagcggcg ggtgtcagct ttgaccagtt tttcccacca agcgtaattt
    42721 ccgctccctc atccgggcga taaacaccgg acataatttt aatcaacgtc gatttaccac
    42781 agccgttttg cccggcaagg cagtgcactt cacccgacat aaagtcgaga gaaacatcgc
    42841 gcagcgctcg cgtggcatga aacgttttgt tgatatgacg aagggaaagg aaagtttcca
    42901 taacatcccc tggcaataag aatcggggct ttcgccccga agaaatggat taataaagtg
    42961 agtcgatatt gtctttgttg accagtaata ccttatggaa acggataatt cgcttatcca
    43021 tatctacgtc ggccttaccc agattctgca tttctaatcc aggtttgatc tcttcaccct
    43081 tcagcaacgt gctggcgacg gctgcgagtg cgtaacctgc agaggccgga tcgtaagtaa
    43141 tcccttcggt gatatcacca cttttaatca gtgatgccgc ctgtgaaggg atcatcatgc
    43201 catagactgc gactttattt ttcgcccgtt tctctttcac cgcacgtccc gcgccaatcg
    43261 gaccgtttga accaaaggag acaaccgctt tcaagtcagg ataggttttc atcaggtcca
    43321 gtgtggtacg acgtgagtca tccacactct cggcaaccgg catgcggcgg gtaacttcat
    43381 gcatatccgg gtaatgctct ttctggtatt tcaccagcaa gtcagcccat aagttatgct
    43441 gcggcacggt caaactaccc acgtaaatca catagccgcc cttgcccccc atgcgtttcg
    43501 ccatatgctc aacatattca gcggcaaatt tttcgttatc aatgatttca atatcccagt
    43561 tagcacttgg ctgaccgggg gattcgttgg tcagaaccac aattccggca tctcgcgctt
    43621 ttttgaatac cggttccagc acgttggcat cgtttggcac gatagtaatt gcattaacct
    43681 tacgggcgat taaatcctca ataattttaa cttgttgcgg agcatcagta cttgaaggcc
    43741 ccacctgtga ggcattaaca ccaaaggctt tacccgcctc aaccacacct tcgcccatgc
    43801 gattaaacca cggcatacca tcgactttag aaatattcac cacgactttt tccgctgcct
    43861 ggagcggcgc agaaattagc gcagcgccta ataacagcga agacaccata ttgataacaa
    43921 aacgtttatt catcatttca gcctcattgc agggtagtca ggaagtgttc gcccaaatta
    43981 gcgcgctgga gagaataaga attttcgaat atttttatgc agaataaaca gccctatttc
    44041 aggcagctat taaaaatatg gttataaata tggacaaaac gggcagcaac aataagtgtg
    44101 acattcatca cg
//
LOCUS       DAZFWP020000017        42561 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFWP020000017 DAZFWP020000000
VERSION     DAZFWP020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.017"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            109..1329
                     /gene="ybdN"
                     /locus_tag="ACHK0Z_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             109..1329
                     /gene="ybdN"
                     /locus_tag="ACHK0Z_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4500955.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1302..1931
                     /gene="ybdM"
                     /locus_tag="ACHK0Z_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             1302..1931
                     /gene="ybdM"
                     /locus_tag="ACHK0Z_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4500956.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1932..3092)
                     /gene="ybdL"
                     /locus_tag="ACHK0Z_RS04455"
                     /old_locus_tag="pgaptmp_000891"
     CDS             complement(1932..3092)
                     /gene="ybdL"
                     /locus_tag="ACHK0Z_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU4500957.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PHYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3201..4289
                     /gene="hcxA"
                     /locus_tag="ACHK0Z_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             3201..4289
                     /gene="hcxA"
                     /locus_tag="ACHK0Z_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU4500958.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNYLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4299..4496)
                     /gene="ybdD"
                     /locus_tag="ACHK0Z_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             complement(4299..4496)
                     /gene="ybdD"
                     /locus_tag="ACHK0Z_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4500959.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4678..6783)
                     /gene="cstA"
                     /locus_tag="ACHK0Z_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             complement(4678..6783)
                     /gene="cstA"
                     /locus_tag="ACHK0Z_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU4500960.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLIWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6964..7377)
                     /gene="entH"
                     /locus_tag="ACHK0Z_RS04475"
                     /old_locus_tag="pgaptmp_000895"
     CDS             complement(6964..7377)
                     /gene="entH"
                     /locus_tag="ACHK0Z_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU4500961.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7380..8126)
                     /gene="entA"
                     /locus_tag="ACHK0Z_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             complement(7380..8126)
                     /gene="entA"
                     /locus_tag="ACHK0Z_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU4500962.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTAFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8126..8983)
                     /gene="entB"
                     /locus_tag="ACHK0Z_RS04485"
                     /old_locus_tag="pgaptmp_000897"
     CDS             complement(8126..8983)
                     /gene="entB"
                     /locus_tag="ACHK0Z_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU4500963.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8997..10607)
                     /gene="entE"
                     /locus_tag="ACHK0Z_RS04490"
                     /old_locus_tag="pgaptmp_000898"
     CDS             complement(8997..10607)
                     /gene="entE"
                     /locus_tag="ACHK0Z_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU4500964.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTLVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10617..11792)
                     /gene="entC"
                     /locus_tag="ACHK0Z_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             complement(10617..11792)
                     /gene="entC"
                     /locus_tag="ACHK0Z_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU4500965.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12167..13123
                     /gene="fepB"
                     /locus_tag="ACHK0Z_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             12167..13123
                     /gene="fepB"
                     /locus_tag="ACHK0Z_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4500966.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13127..14377)
                     /gene="entS"
                     /locus_tag="ACHK0Z_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             complement(13127..14377)
                     /gene="entS"
                     /locus_tag="ACHK0Z_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU4500967.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14488..15492
                     /gene="fepD"
                     /locus_tag="ACHK0Z_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             14488..15492
                     /gene="fepD"
                     /locus_tag="ACHK0Z_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU4500968.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15489..16481
                     /gene="fepG"
                     /locus_tag="ACHK0Z_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             15489..16481
                     /gene="fepG"
                     /locus_tag="ACHK0Z_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU4500969.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLSWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16478..17293
                     /gene="fepC"
                     /locus_tag="ACHK0Z_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             16478..17293
                     /gene="fepC"
                     /locus_tag="ACHK0Z_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4500970.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17290..18423)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK0Z_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             complement(17290..18423)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK0Z_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU4500971.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(18639..22520)
                     /gene="entF"
                     /locus_tag="ACHK0Z_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     CDS             complement(18639..22520)
                     /gene="entF"
                     /locus_tag="ACHK0Z_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU4500972.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADAHYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22517..22735)
                     /gene="ybdZ"
                     /locus_tag="ACHK0Z_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             complement(22517..22735)
                     /gene="ybdZ"
                     /locus_tag="ACHK0Z_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGU4500973.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22738..23940)
                     /gene="fes"
                     /locus_tag="ACHK0Z_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             complement(22738..23940)
                     /gene="fes"
                     /locus_tag="ACHK0Z_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU4500974.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24183..26423
                     /gene="fepA"
                     /locus_tag="ACHK0Z_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             24183..26423
                     /gene="fepA"
                     /locus_tag="ACHK0Z_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU4500975.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYVTTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSKNKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26463..26561
                     /locus_tag="ACHK0Z_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /pseudo
     CDS             26463..26561
                     /locus_tag="ACHK0Z_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26598..27218
                     /gene="entD"
                     /locus_tag="ACHK0Z_RS04555"
                     /old_locus_tag="pgaptmp_000911"
     CDS             26598..27218
                     /gene="entD"
                     /locus_tag="ACHK0Z_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU4500976.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27340..27492)
                     /gene="hokE"
                     /locus_tag="ACHK0Z_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     CDS             complement(27340..27492)
                     /gene="hokE"
                     /locus_tag="ACHK0Z_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4500977.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27843..27995)
                     /gene="hokE"
                     /locus_tag="ACHK0Z_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             complement(27843..27995)
                     /gene="hokE"
                     /locus_tag="ACHK0Z_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4500978.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            27964..28233
                     /locus_tag="ACHK0Z_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             27964..28233
                     /locus_tag="ACHK0Z_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4500979.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            28437..29555
                     /gene="ybdK"
                     /locus_tag="ACHK0Z_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     CDS             28437..29555
                     /gene="ybdK"
                     /locus_tag="ACHK0Z_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU4500980.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29621..29869
                     /gene="ybdJ"
                     /locus_tag="ACHK0Z_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     CDS             29621..29869
                     /gene="ybdJ"
                     /locus_tag="ACHK0Z_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU4500981.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29934..30302
                     /gene="ybdF"
                     /locus_tag="ACHK0Z_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     CDS             29934..30302
                     /gene="ybdF"
                     /locus_tag="ACHK0Z_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4500982.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALIRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30396..31049
                     /gene="nfsB"
                     /locus_tag="ACHK0Z_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     CDS             30396..31049
                     /gene="nfsB"
                     /locus_tag="ACHK0Z_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU4500983.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31157..32404
                     /gene="ybdG"
                     /locus_tag="ACHK0Z_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     CDS             31157..32404
                     /gene="ybdG"
                     /locus_tag="ACHK0Z_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU4500984.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32472..33848)
                     /gene="pheP"
                     /locus_tag="ACHK0Z_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     CDS             complement(32472..33848)
                     /gene="pheP"
                     /locus_tag="ACHK0Z_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU4500985.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33950..37093)
                     /gene="cusA"
                     /locus_tag="ACHK0Z_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     CDS             complement(33950..37093)
                     /gene="cusA"
                     /locus_tag="ACHK0Z_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU4500986.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSDKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37105..38328)
                     /gene="cusB"
                     /locus_tag="ACHK0Z_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     CDS             complement(37105..38328)
                     /gene="cusB"
                     /locus_tag="ACHK0Z_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU4500987.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38344..38676)
                     /gene="cusF"
                     /locus_tag="ACHK0Z_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             complement(38344..38676)
                     /gene="cusF"
                     /locus_tag="ACHK0Z_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU4500988.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38700..40082)
                     /gene="cusC"
                     /locus_tag="ACHK0Z_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             complement(38700..40082)
                     /gene="cusC"
                     /locus_tag="ACHK0Z_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU4500989.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40239..40922
                     /gene="cusR"
                     /locus_tag="ACHK0Z_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             40239..40922
                     /gene="cusR"
                     /locus_tag="ACHK0Z_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU4500990.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40912..42360
                     /gene="cusS"
                     /locus_tag="ACHK0Z_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             40912..42360
                     /gene="cusS"
                     /locus_tag="ACHK0Z_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU4500991.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10155 a        11769 c        10895 g         9742 t
ORIGIN      
        1 tattcacagc gcgaatgaaa aataaaacac tctgctttgc agtgtttact ttataattaa
       61 cagacttatc tcactgatca ccctgtaacg ttcagagagc gtcttcagat gtctatttat
      121 aaaattcctc ttccgctcaa tattctggaa gctgcacgag agcgtattac ctggacactc
      181 aacaccctac cccgcgtttg tgtctcattt tctggcggca aagactctgg tttaatgctg
      241 catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt gtttatcgac
      301 tgggaggcgc aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc
      361 gatgtcatcg aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca
      421 caattccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg
      481 caagatgcga taaccgatcc tgactttttc tgcttttacc agcctggcat gaccttcgaa
      541 caatttgtac gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc
      601 ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa
      661 cgttttgccg acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt
      721 taccccattt acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaagt
      781 ctctgcaacc cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga
      841 atttgcgaac cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa
      901 ccggatcgct gggcagctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac
      961 gccggacatg acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac
     1021 tggcaagaat atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac
     1081 cgcaataaaa tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa
     1141 acccagcaag gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt
     1201 ttactcaata acgattactg gtgtcgagca ttatcattta gtcctacgaa agcgaagaac
     1261 tatcagcgtt ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac
     1321 aacgattaac gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca
     1381 aagcgattaa tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag
     1441 tggattgcgt tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca
     1501 acgtggcacc acccgaaaaa aaactgctga aaaaatccat tgaaatagat ggttttaccc
     1561 aacccattgt ggtcacgcat acggataaaa atgccctgga aatcgtggat ggttttcacc
     1621 gccatgaaat tggtaaaggc tcatcatcat taaaattacg cttaaaaggt tatttgccgg
     1681 tgacctgtct ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt cataatcgcg
     1741 ctcgtggacg ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt agccagttgg
     1801 gatgggacga taataaaatt ggcaaagagc tgggaatgga cagcgacgaa gtgctgcgcc
     1861 tgaagcaaat taacggcctg caagagttgt ttgccgaccg tcaatattcc cgcgcctgga
     1921 cggttaaata gctaaagctg gcgcaggcgt tcagctgctg ccagcaacgt cgattccttc
     1981 ttggcaaaac agagacgaat cagtttatgt gggaagggat cggcgcaaaa caccgacagc
     2041 ggaatcgccg ctacgccgtg ctcctgcgtc agccactggc aaaactcaac atcatccagg
     2101 gtagaaaccg cgctgtaatc caccagcaaa aagtatgtac cttcacacgg taaaatctcc
     2161 agccggcttt catttaaagc attcaccaga atatcgcgct tctggcgata aaagtccggt
     2221 aacgcaagat aatgctcagg ttctgcacgt agcatatcag caagcgccag ctgtgccggg
     2281 gtattcaccg aaaaggtcag atactgatgt accttgcgaa tttcggcgct gatgggcgct
     2341 ggcgcaacac aataacccac tttccagccg gtcatatgat aggtcttgcc aaatgaagaa
     2401 accgccactg cccgctcacg cagctgcgga tgcgccagca cactggcatg gccctgttgt
     2461 gaaaagttga tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg cccggcgatc
     2521 gcctgccaca aagcggcgaa atcagcctgc tgccagatag ttgcactggg gttatgcgga
     2581 gtgttgagga tcaccagtct ggtgcgctcg cttaataatg cggcaaattc ctgccagtca
     2641 acgcgaaaat gcggtggttg cagtgccata cgcttcacta ttcccccaga aagcgcgatg
     2701 gcgggggagt aactgtcata gctgggatca aaacaaatca cttcatcgcc attgcgcacc
     2761 agtgcggtaa tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt gatatcgcta
     2821 tcggcatctg gttgatagcc atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag
     2881 gcctgcacgc cggtcatggg cgcgtattgg tttgccccct gggcaacgtg gtacgccagc
     2941 cgctcctgta aatagtgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc
     3001 tggtgttgct gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt
     3061 ttgctttgtg gaatcagagg gttatttgtc atttttattg tacctgacga agttgcgtgt
     3121 tgaaggcact ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa
     3181 aacaataagg acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta
     3241 actacttttc acatccagga agtttcaatt acctgaacga ttttttcact gatgaacaac
     3301 tttctcgcgc ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa accaaacttc
     3361 cgccagcgtt tggacttcca ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa
     3421 gcgatgtaca gcaactggcg gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg
     3481 gcggcggcgc actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctaccatttg
     3541 ttgccgctcc gacgatcgcc gccacctgcg cagcctggac accgctctcc gtctggtata
     3601 acgatgccgg acaggcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg
     3661 tggaaccgga gattatcctc aatgcaccgc aacaatatct gctggcgggg atcggtgaca
     3721 cgctggcgaa atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa
     3781 ccgtgcggct ggggatcaat aatgcgcagg ccattcgcga cgtcttgtta aacagtagcg
     3841 aacaggcact tgccgatcag caaaatcagc agttaacgca atcattttgc gatgtggtgg
     3901 atgcgattat cgccgggggt ggaatggtcg gtggtctggg cgatcgtttt acgcgtgtgg
     3961 ctgcggccca tgccgtgcat aacggtctga ctgtcctgcc gcaaaccgag aaatttctcc
     4021 acggcaccaa agtcgcttac ggaattctgg tgcaaagcgc cttgctgggt caggatgatg
     4081 tgctggcgca attaaccggg gcgtatcagc gttttcatct gccgactacg ctggcggagc
     4141 tggaagtgga tatcaataat caggcggaga tcgacaaagt gattgcccac accttgcgcc
     4201 cggtggagtc cattcattac ctgccagtca cgctgacacc agatacgttg cgtgcagcgt
     4261 tcgaaaaagt ggaatcgttt aaagcctgac gtgcaaattt agcagcagcg cgcgccgcct
     4321 tttccaccgt agcgcgcgtc ctgccgctca cggaaaaact cctcgtaggt catcggcgtt
     4381 tgatcgggat ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc
     4441 atcagcttcg ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcttg
     4501 gcctccgcta accatgttgc tgaaagagtg ccggatgcgg cgtgaacgct ttatccggcc
     4561 tacaaatgct atcaacgcac tcggtcagtc ccctcgcccc atcggggaga gggttagggt
     4621 gagggggaac cctcggcact atgccaacat tcgccaacat cccccctcac tctgacttta
     4681 gtgcgcacct ttcgcctgcg ctacgatctc ctcaacattt tccggcattg gctcatacgg
     4741 cgtttctttc gccgttggct tcggctcttt caatgccgca agtgccgtct taatcgagaa
     4801 cagtgccaga accaccacga ccaccataaa gaagatggtt aacccggcat ccagacggtt
     4861 gttgaacacc agttgcgcca gctgtgactc agtatactgc gacggaatat tgccgctgtc
     4921 gatcattgcc tggaacttgt tagcaatggc caggaagccg actttcgcat ccgggctaaa
     4981 cgctttctgc cagcctgcgg tcagggtaca aatcagtagc caggccgttg gtaccagcgc
     5041 cacccaggcg taacgttgac gtttcatctt gaacaacacc acggcacaga gcatcagcgc
     5101 catccctgcc agcatctggt tggcaatacc aaacagcggc cacagagtgt taataccgcc
     5161 cagcggatcg accacgccct gatggaggaa gtagccccac gccagtacgc acagcgccgt
     5221 tgccagcagg ttagcaggca gtgaatcggt ccgcttcagg ccaggagaca ccacgcccag
     5281 cagatcctgc aacataaagc gcgcagcacg cgtacccgca tctaccgccg tcagaataaa
     5341 cagagcttca aacagaatgg cgaagtgata ccagaacgcc acatccatca taccacccag
     5401 cgcaccgtgc aggatgtaag ccatccccac cgccaacgtc ggcgcaccgc ccgcacggga
     5461 aatgatcgac tgttcaccca cttcgctggc aatctggttg agcgtatctg gtgtaatgct
     5521 aaagccccag ctactcacca cctgcgcggc agaagcgacc acatccgccg tccctgccgg
     5581 agccagcacc gccattgggc tgttcatggc aaagtaaacg cccggatcga tgatacaggc
     5641 ggaaaccagc gccataatcg ccacgaagga ttccatcaac attccaccgt agccgataaa
     5701 acaagcctgc ccttcgttcg ccagcatctt cggcgtggtg ccggaagaga tcagcgcatg
     5761 gaagccagac accgcgccac aggcgatggt gataaacagg aacgggaaca ggttaccggt
     5821 ccataccggg ccagtgccat cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg
     5881 cataatcaaa atgcctaccg ccagaccgac gatcgtcccg attttcagga aggtagagag
     5941 gtagtcacgc ggggccagca gcaaccacac cggcagcacc gccgccacaa aaccgtaacc
     6001 caccagcatc cagatcagct gcacgccggt aaagtcaaag tacggtgccc aggtcggact
     6061 ttctgccacc cagccgccag agataatggc gaaaatcagg aataccagac cgatgaccga
     6121 cacttcacca atacgccccg gacgcagata gcgcaggtag atccccataa acagcgccag
     6181 cggaatggtg aacgcaacgg tatacgttcc ccacgggcta tgagtcaggg ctttcaccac
     6241 gatcatcgcc agtactgcaa ggataatgac catgatcata aagcaggcca ccagcgctat
     6301 caccccggcg gttggcccca tctcttcttt gaccagctca cccagcgatc gaccgtcacg
     6361 gcgcgtagaa acaaacagca ccatgaaatc ctgcaccgca ccggcgagaa ccaccccggc
     6421 gagcagccag atcatccctg gcaggtaacc catttgcgcc gccagtaccg gcccaaccaa
     6481 cggacctgct ccggcaatgg ccgcaaaatg gtgaccgaac agcactttct tgtccgtcgg
     6541 cacatagtcc agcccgtcgt tatggcgcac cgctggcgtc atacgcgtcg ggtcaaccgc
     6601 cagcacattt ttggcgatat acagcccata aaaacggtaa gcgatcagat agatacagac
     6661 cgacgccacc acaatccaca gcgcgttgat ctgttcccca cgatttaaag caatgtatcc
     6721 cagagcaaat gctcccatta cagagagcac tgtccagacg aggtatttcc ctgatttgtt
     6781 catagttgtt atccgtgtgc gtgtccatag atatgttaca ttttgtttct ataacatctt
     6841 tgtacgattt aacaacacgg taaccatgta aatcggagtt gaaaccaata tttaacttaa
     6901 caatgttaac tcgatcactc cgttaaccga gttttcctgc aatctcattt aattctgtcg
     6961 ggctcatccc aaaactgccg tacccagccg acaagtgcag caacgccgcc cctgttcatc
     7021 gaaaacgacg atttcccagc tctgattttg ccgcccaaga tgcagcggct ggcagacgcc
     7081 gcgtaccttg ccttcagaca ccgggcgatg atgggtggcg ttaagttccg tacccaccac
     7141 acactgcccg tcacgggtca tcataaatcc ggccatcgat cccagcgttt cagccagcgc
     7201 cgctgacgcg ccgccatgca gcaggccaaa cggctgatga gtacgggtat caaccggcat
     7261 ttcggcttcc agcacatcat ctcccagacg ggtatacaca attcccagat gcgccaccat
     7321 tgtgttatcg ctggtggcgt tcagttcgtc gagcgttaaa tggcgtttcc agatcatgct
     7381 tatgccccca gcgttgagcc gccatcgacc acaatatcct gtagggtgat atggctggcg
     7441 aggtcagagg cgaggaacaa aatcgtgttg gcgatctctt gtggacgggc gattttcccc
     7501 agcggaatgc cgagtttaaa ctgctcgcca aagccgcgaa tacgctgttc ttcggcgtca
     7561 tcgctcaccc acagcgtgcg ttgcatatcg gtgtcggtgg agccaggcga aaccacatta
     7621 cagcgcacgc cgctacccgc cagttccagc ccgacgctca acgccaggct tttcagcgcc
     7681 gctttcgatg cgccataagc actcatgcca atacgcggcg tgtgcgcggc gtcggacgcc
     7741 acagtgacaa tcgccccgcc ccgctgacgg cgaaactggt tcatggtttg ctggaacagg
     7801 ttaaacgcac cgccgacgtt aaccgcaaaa gtctgctgcc agtcctcttt actgagctga
     7861 tcggtcgcgc ccatgcgcaa aattcccgcc gcattgacca gcacgtccag tcgctccgtt
     7921 tcagctaaca gtcgctgaca tacctgcgcg acctgcgcag catcggcaac atccatcact
     7981 tcggtcgcaa agggatattg ctcctgagcg aacgcttgat caaaagcggt aactttcgct
     8041 cccgcctcaa caaacgccag cgccgtggcg tagccgatac ctttacctgc gccggttacc
     8101 cagacatttt taccgctgaa atccattatt tcacctcgcg ggagagtagc ttccaccagg
     8161 cgtcgatggt cgggtttttc gccagcatga caaagtcgat atcaccatgc actttgcgcc
     8221 agcgcgccgc cagcgccatc atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt
     8281 catcgaacgg ttcatcggac tcgtccagca acggcaggat aacctcacgc agcgccgctt
     8341 tgctggcggg gacaggtgct ggcagtaatt cttcagtcat caccacccgg ccagaccgcc
     8401 cggccacata tttcagcgac atcaaatgct cgtcacggct gaaatcggcc agcgcatccg
     8461 ccaccataaa cggtttaata tcgcgcataa atgcgtcggt tgcggtggtc atacagccaa
     8521 tgtgggcata taccccggta ataatcagct ggttacgtcc actctctttg agcatttgct
     8581 ccagcggaga acgatgaaac gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat
     8641 ctggcgtcag gcgatccacc accttttgct gttccggcga acgggtcagg cccggccccc
     8701 acatatcatt caacagcgcc cgatcttcat cgctctgctc tttcggctgg gcggtgtaat
     8761 aaaccggaat attgtgctgt ttgcagtagt cgcgcagcgc agcaatattc gcgatcacct
     8821 gctccatcat cgggcagttc tcgccccaga agctgacaaa atagtcctgc atatcatgga
     8881 ttaacaacgc ggcacgttgc ggttcaaagg cccagtcaac tttattctta gggatatcgt
     8941 gagactccgg cagtgcgtaa gcctgtaatt ttggaatagc catcgtgttc tctccttcag
     9001 gctgatgcgc gtgacgccag ccactgacgt aattgttttt tatcgacttt cccgaccgcc
     9061 gtcagcggaa gtgaatctac acactccacg cgatccggta atttaaattc ggcaataccc
     9121 tgttcacgca ggaaacgacg cacctgcacc gcgcgcagcg gctcttttac caccagataa
     9181 gcgcagcttt tttcgcccat cagctcatct tccatgctca ccagtgcggc gtagatcacc
     9241 gccgggtggc gcagcagcag gttttcgatc tcttcggcag cgatcttctc accgccacgg
     9301 ttgatctgat ctttctcgcg cccctgcacg gtgatgtacc cctctggatc aatagagatc
     9361 agatcgccgg aacagtaaaa accgttggca tcaaaggcac tggcattgtg ctgcggactt
     9421 ttgtaataac cgcggaaggt gtacggcccg cgcgtcatca ggcgtccaac ttccccttgc
     9481 ggcagtggat ttccttcagc atcagcaacc catacttcat cgtccggaca cattgggtaa
     9541 ccctgggtat ggataatttt ctccgcgcta tcatcaagtc gggtgtagtt caccagccct
     9601 tccgccatgc caaacacctg ctgcaacagg cagccaatct cagcgggaat acgcgccgca
     9661 agggtggctg aaagacgtgc gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc
     9721 cggctttcac cttcggtcag cgcctgcaac cacaggctga ctgccggtgg caccagcgcg
     9781 gtgacgttca cctgatgttt ttcaatcaat gggaagcaaa gcgtggcgct gggatcggcc
     9841 gccagaacaa gcgttcctcc ggcaagaaag acgcccagcg atcccggcga actcatggcg
     9901 tagttatgag ccgccgggat cgcgcacagg tagcgtgtct gttgtgtgaa ctgacaaatc
     9961 tcgacgctac gacgcacgct gtagtagtag tcgttatgag tgcgcgggat cagtttcggt
    10021 gtgccggtgg tgccgccgga aagctggaaa taggccactt catcagcagg tgatggcgtg
    10081 gcagtaaaat cctcagccgg atggttaatc gcatcctgca agttatgctc accgctgtcg
    10141 ttgtgcagtt gcaccacgcg aatggaggaa tgttctgtga cgaaagtatt gaggaaatca
    10201 tccccgctaa acagcgcatg ttggcgatcg gcaatcagca atgcgggttc aatctggctg
    10261 gcataggcgt tcagttcact acgctgatgg ctgaacagcg ccagcaccgg cgcaacgccc
    10321 agtttcagca gcgcgaaaaa ggtaatatac aattcagcga cgttacccag ttgtaccagc
    10381 gcggtttcac caggtttaat gccctgacgg cgtaaactac acgcgaggtt atccgccgcc
    10441 tgattcagct cccggtaact caactgtcgc tcgccgtcga taaccgcgat gctgtcactc
    10501 gcagcgtggc gagtcagaat gtcggtcagc ggcaaatcct gccagtagcc tttttcccga
    10561 tagcgacggg caaactcttc cggccagcgg gtgaatggaa tgctcatcct cgctccttaa
    10621 tgcaatccaa aaacgttcaa catggtagaa agtttgacgc ctgtttcgcg ccactcaccc
    10681 aacggtgacg acgcaggcac aatccccgct ccggcaaaca gacgcacctg attttcccgc
    10741 agcttcgcgc agcggatggt caccacccat tcgccgttac cttcgctgtc acaccaaccc
    10801 acaatgccgc caaacagttc gcggtcgaat ggctccagtt cagcaataac ctgggtcgcg
    10861 gcctgatgcg ggaaaccgct cagcgcgggg gttggatgca gcagacaggc cagagtcagt
    10921 gcgttttctt gcgaattcgc tttaccttca aagggagttg cgagatgcca cagcgtcggc
    10981 gtggtaatca attgtggaga ggagggaacg tgtaactcac tactgcgttc gcgcagtacc
    11041 tctttcatcg cctgagtcac cagttcatgt tcatggcgat ctttttctga cgccagcaga
    11101 cgattacccg cttcgcgatc gagcacttca tccggctgac gacgcgcgga accggctaac
    11161 ggaatggagc taaaacgctc gccgtcttta cgtagcagca gttccgggct ggcccccagc
    11221 aggacgccac catcagccag cgggacatgg aagttgtaac taaccgggtt ttgcgcaatc
    11281 aaccgttcca gcaatacgcc actatcaatg gcggcgtcag tggtgatatc aatcaatcgt
    11341 gacaacacca ctttgtcgac ctgcggcgtg gcggtaagtg cggcggcgcg ggcaaccatc
    11401 tgttcaaacg tggtttgttc aggaattgcc tggcgttcca ccacgttcag cgactggctg
    11461 cgggtaaaac ggcgggctga ggtctgtttt tcctggcggg agaacgactg ccaggattcg
    11521 ggaatataca gcgacgaagg ctgacgtgga tcgaagggaa tcgccccgac catcaccgga
    11581 tttttgatgc cctgcgcttt ggcatcggca aacagcgcgg cgagtttttg ctggaaggga
    11641 ctgtcgggcg aatccccgtt cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc
    11701 gtaaaactgc ggtacggcga cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc
    11761 tgctgtactt cctcagccag tgacgtatcc atatcatcct ccacaaaatg ataaaggctt
    11821 taataatgat tatcatttat attttcggtc gctaacctaa aagcaaacgc gcactatgtc
    11881 aactcttgag gtaacgcaat ctgcaactcg gggttgcatc tggttgtggt tacaatgaaa
    11941 atgagaagca ttattgatgg attcgcataa gcgcaatgtg atgtcctgcg ccgttctgcc
    12001 ccctctccct tccagggtga gggctggggt gagggttaat gttcgcacca gtgctggctg
    12061 ttcccctcac cctaaccctc tccccaaagg ggcgagggga cggattgtgc gctttgtcga
    12121 atttgtcatt acgcccttaa ccttattaat aacaggaagc tgatttgtga gactcgcccc
    12181 gctctaccgc aacgcccttc tattaacagg acttttgctt tcaggaatag ccgcagttca
    12241 ggccgctgac tggccgcgtc agattactga cagccgtggc acacatacac tggaaagcca
    12301 gccgcagcgt attgtttcca ccagcgtcac cctgaccggc tcactgctgg cgattgatgc
    12361 tccggtgatc gccagcggcg cgaccacgcc gaataaccgc gtcgcggatg accagggctt
    12421 tttacgccag tggagcaagg tggcgaaaga acgcaaactg caacggctct atatcggcga
    12481 accgagcgcc gaagccgttg ccgcgcaaat gccggatctg attttaatta gcgcaaccgg
    12541 cggggattcg gcgctggcac tgtatgatca gctttccacc atcgccccga cattaatcat
    12601 caattacgac gacaaaagct ggcagtcgct gttaacgcag cttggcgaaa ttaccgggca
    12661 tgagaaacaa gcggcagagc ggattgcaca gtttgataag caactggcag cggcgaaaga
    12721 gcaaatcaaa ttaccgccgc agccggtcac tgccattgtc tataccgccg ctgcacacag
    12781 tgccaatctc tggacgccgg aatcagcaca agggcagatg ctggaacaac tcggctttac
    12841 gctggcgaag ttgcccgcag ggttaaacgc cagccaaagc cagggcaaac gccatgacat
    12901 cattcagctt ggtggggaaa atctggctgc tgggttaaat ggcgagtcac tattcctgtt
    12961 cgccggtgat cagaaagatg ccgatgcgat ttatgccaat ccactgctcg cgcaccttcc
    13021 cgcagtacaa aacaagcagg tttatgcgct gggaaccgag acgttccgtc tggattacta
    13081 cagcgccatg caagtgctgg aacgacttaa ggcgctgttt taagcattaa ctgtcagacg
    13141 ctgtcacctg cggcggcgtc tggcgaaaac gtcgcaactc caccagcacc agcaataaca
    13201 acacgccgat aatcaacaaa ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca
    13261 tcgcgcccaa accacccagc agcgccgcgc ctatggcatc gcccgtcacg ttctgcgccg
    13321 tccacaaacc gttaatccgc cctaacatcg cttccggggt ttgcgtttgc agcattgtat
    13381 attgcagcaa cgaactaacc gcgctcagcc agccgaacag cgccagacaa accacgccta
    13441 aaatccacat cggcatcagg ccaaacagac caatggcgag gaacgatccc agcgtggaga
    13501 gcagcatcaa taaccctggt cgcgcactat gtgccagctt cccgctggtt aacgcaccaa
    13561 tagccgcgcc gagcgggatc gccgcgtaga gaaaaccaat ctgtgccgct gacatctgcc
    13621 agttgtcagc cagcgccgga tacagtaccc gcaccgcgct cgccatcgtc aataaaccac
    13681 ccagcagcgc aatcccgccc accagcgggc tggcaagcag aaaacgaaat cctgccagta
    13741 atgatttcaa cggatgctca cgcggctgcg gtggcggtgg caacgccgga aggcttaaca
    13801 acggtagcaa ggtaataaac gtgcccgccg ccgccagccc gtagttccag gctacgccac
    13861 cggtcgccag caataaaccg ccaatcatgg gcgaaatcac cgaccccaga cgcacggtca
    13921 acatggtgat cgccccggcc tgcattaagt tttcacgccc taccagtgca ggtgtcgccg
    13981 ccagtagcgc cgtaacgcca agtgatgcga aaaaaccatc ccataaacca agtaaataga
    14041 ttgccagcaa tgacggttca ggcagcagtg cattaaggca cagtccaatg aagccaatgc
    14101 cacaggtgcc gcgcgccagc aaaatcactt ttttgcgttc atagcgatcc gccagcacac
    14161 cgccgaccat caggccaaca aacatcgcgc cgccggtcag cgtcaccgaa agccccacct
    14221 gccaggtaga atgtgtcatc atctggatct gcaccggcac cgcgacgccg agcaaaccca
    14281 gagaaacaat agagatgaaa cgagcgagga atactgcgcg aaacgccggg tgcgttttca
    14341 acaggctgag gttaagcagc caggattgtt tattcattac aatgccttgc catcaaatat
    14401 tttaaatacc tattcttagg ctgcacatgc taacatatcc aaataagatc gataacgata
    14461 attaatttca ttatcatgga agttcgtatg tctggttctg ttgccgtgac acgcgccatt
    14521 gccgtgcccg gattgctgtt attactgatt atcgcgacgg cattaagtct gctcattggg
    14581 gcaaaatcac tccccgcttc cgtagtgctg gaggccttct ccggcacctg ccagagcgcc
    14641 gactgcacca tcgtgctcga cgcgcgtctg ccgcgtaccc ttgccggttt actggcaggc
    14701 ggcgcgcttg gccttgccgg ggcgttaatg caaaccctca cccgtaaccc acttgccgac
    14761 cccggcttgc ttggcgtgaa cgccggagcc agctttgcca ttgtgctggg tgcggcgctg
    14821 tttggttact cttccgcaca ggaacaactg gcgatggcct tcgccggggc gctggtggcc
    14881 tcgttgattg ttgcctttac cggcagccag ggcggcgggc agttaagtcc ggtgcgttta
    14941 accctggcgg gcgtggcgct ggcggcggtg ctggaaggac tgaccagcgg catcgccctg
    15001 cttaatcctg acgtctacga tcaactgcgt ttctggcaag ccggttcgct ggatattcgc
    15061 aatctacata ccttaaaagt ggtgctgatc ccggtgctga tcgccggagc aactgcgcta
    15121 ttactgagtc gcgcgctgaa cagtttgagc ctcggcagcg acaccgcgac ggcgctgggc
    15181 agtcgcgtgg cgcgcacaca gttgattggt ctgctggcga ttaccgtgct ttgtggtagt
    15241 gcgacggcag tagttggccc gattgccttt attggcctga tgatgccgca tatggcgcgt
    15301 tggctggtgg gtgccgatca tcgctggtcg ctgcccgtca cgctacttgc tacccctgcc
    15361 ctgctgctgt ttgccgatat catcgggcgg gtgattgtgc ccggcgaact gcgcgtttct
    15421 gtggtcagtg cgtttattgg tgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga
    15481 ggtggtgcat gatttacgtc tctcgccgat tactcatcac ctgtttgctg ctggtttccg
    15541 cctgtgtggt tgcaggtatc tggggattac gcagcggtgc cgtcacgctg gaaacctcgc
    15601 aggtattcgc cgcgctgatg ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat
    15661 ggcgtttacc acgcgtgctg atggcgctgt tgattggcgc agcactgggc gtcagtggcg
    15721 cgatttttca gtcgctgatg cgtaacccgc tcggcagccc tgacgtaatg ggctttaaca
    15781 ccggggcgtg gagcggcgtg ctggtggcga tggtgctgtt tggtcaggac ctgacggcta
    15841 tcgcgctgtc agcaatggtg ggcggcattg tcacttcgct gctggtctgg ctgctctcct
    15901 ggcgcaacgg catcgacacc tttcggttga ttattatcgg tatcggcgtt cgcgccatgc
    15961 tggtggcctt taatacctgg ctgttgctga aagcgtcttt agaaacggcg ctaacagcag
    16021 gtttgtggaa tgccggatcg ctcaacggcc tgacgtgggc aaaaacctcg ccttccgcac
    16081 ccatcattat attgatgctc attgccgccg ccttactggt acgccggatg cgcttgctgg
    16141 aaatgggcga tgataccgcg tgtgcgctgg gcgtcagcgt cgaacgttcg cgtctgttaa
    16201 tgatgctggt tgcagtggtg cttaccgctg cggcaacagc gcttgccggg ccgatttcct
    16261 ttattgcttt agtcgcaccg cacattgccc gacgcattag cggcaccgct cgctgggggc
    16321 taacccaggc ggcgctatgc ggggcgctgt tactgctggc ggccgatctc tgcgcccaac
    16381 aactgtttat gccgtatcaa cttccggttg gcgtcgttac cgtcagcctc ggcggtattt
    16441 accttatcgt cttgttaatt caggagtctc gcaaaaaatg accgaatcag tagcccgttt
    16501 gcgcggcgaa cagttaaccc tcggatatgg caaatatacc gttgcggaaa atctgactgt
    16561 agaaatacct gatggtcact tcacggcaat tatcgggcca aatggctgcg gtaaatccac
    16621 gttactgcgt accttaagcc gcctgatgac gcctgctcat gggcatgtct ggctggatgg
    16681 cgagcacatt caacattacg ccagtaaaga ggttgcacgc cggattggtc tgttggcgca
    16741 aaatgctacc acgccgggcg atatcaccgt gcaggagctg gtggcgcgtg gacgttatcc
    16801 gcatcaaccg ctgtttaccc gctggcgcaa agaggatgaa gaagcggtaa cgaaagcaat
    16861 gcaggccacg ggaataactc atctggcaga tcaaagcgtg gatacccttt ctggcggaca
    16921 acgccagcgc gcgtggatcg cgatggtgct ggcccaggaa acggcaatta tgctgctcga
    16981 cgaacccacg acctggctgg atatcagtca tcagattgat ttgctggagt tgttaagcga
    17041 actgaaccgc gaaaaaggct ataccctggc ggcggtgctg cacgatctta atcaggcctg
    17101 tcgttacgcc agccatttga ttgcattgcg ggaagggaaa attgttgctc agggagcgcc
    17161 gaaggagatt gtcactgctg aactgattga gcgcatttat ggtctgcgct gcatgatcat
    17221 tgacgatcca gttgccggaa cgccgcttgt ggtgccgctc ggacgaacgg caccttcaac
    17281 cgcaaatagt taaactaagt ggtctgccat catggcatcc tgttttctgg atgccatcgc
    17341 atagcgcaac agcacgccac cacaagccac catcccgccg atcaacgcgg aaaggatcac
    17401 aataatcgcc ttacccggac cgtctttttt cactggcaat gacgggctta actgatattt
    17461 aaacggcgta aaattcacat cgttgacatg tgcttttgtt aattgctcga caagatactg
    17521 ccgattacgt aattcaccgt tcagttccgc aacgtcagtg accgcttttt ctatttccag
    17581 tttgcgttca ataccgtctg caccgagaga aatagaaaaa tcggggtcat ctttaacggc
    17641 ctgaccatta ctgtaaacgg gctttttaat tcctgccgcg ttggcaatgt cgagtgaata
    17701 attgaggcgc tgaatgtttg catcaagttg atttttcgtt ttaatgcgat cctgagccag
    17761 tttttctttt tcaaactggg ttttgatctc cagtttatta cggacgtttt ctagcgactc
    17821 tttcaccacc aacgtagaga tataatcgat atacccgctc aaaacggtct gcgcctcttc
    17881 actggttggc gcggtaaaac ttagcgtcca ggaggtatac agtgacggtt catctttttt
    17941 cttactggca ttgtcatcaa ccgctttcat tttttcgctc aatgcgacaa ttgcgcgatg
    18001 caaatccagt tcgtcgattt tcgcctcttt taattggtcc atcacataag gtgatgaacg
    18061 caggtactct tccagcaagc taaccgactg aaacttcttg ataaacaggt taaatgcttc
    18121 tgtacgatca attttgatat ccagatccag cacacgaagc ttagtgaatg ttttctccaa
    18181 ctcttgccac tgaacaggtt ctggaggcgt gacaaccgcc gcgctggtcc atttttgcgg
    18241 caggatgaaa gagatcagca agcctgcgca ggcaaacgca aaaacgaccg ccatgaccgt
    18301 ttttttggcc cgccataaaa ctgagattag attaagtaaa tcaatttcat tattactggg
    18361 cgacgccaga ggataatcgg gaaaatgagc gtcacttccc tgtttaatat tcagtgatga
    18421 catggggtat aacctgaaaa tgagtccaat agggggaaat ctccacggcg ctcactttac
    18481 cagttggtga gaattatccg aagctgaagt ttgtaaattc cttccaatga atgcaacatt
    18541 cactctattt aagagaaata accagcaaac ttataaatta aacaaatgat taacccataa
    18601 ttatttcctg taattatggg ttaatagata atgttaattt acctgtttag cgttgcgcga
    18661 ataatcggcc caattttttc aaacgcccct ggagagataa tatccacatg cgcacaatcc
    18721 tgacgataga tatccagttc cgctatccac ggcgaccagg cgcgttcggg actcatacct
    18781 tcctgaagtg tgcgttcagc aacaaacagc gtcgctttac cgtcaaacgg tacgctatga
    18841 gcagtcgtca gcaggcgcac agcatcagcg tagttgcctt caatggtggt aaacaactcc
    18901 gttgaagtac ttccctgctg tgctgccagg aaggcctcgc gttcgcggtt aatctccgcc
    18961 agcacttccg ggtccagacc attagcttct ttttcctgcc agttttgcgt ttctggcggc
    19021 caggtatcca gcaagccaag aaatgccacc tgttcgccac gggcacgcag tcgcgccgca
    19081 ataccctgcg ccagcgtacc gccaagggaa taccccagca ggtaataagg gccatgcggt
    19141 tgttgttcaa gtaacgttgc cagatgcgct tcgcagactt catccaggtt tgccgccgtc
    19201 tgcatggggc cattggggcg cggtgactga atgccgataa tcgaccattg tggatcgaga
    19261 taacgcgaga gcacgctgaa ctgccaggca aaaccggacg caggatggaa acaaaacagc
    19321 gtcgggccat taccttcacg caacggcaga atggtttcga atcccatacg ccgggtgctg
    19381 tcttcttcag catcaataat cgttgccagt ttggcgacag ttgacgcgac catcacttgc
    19441 cccggcgtca cctggcgggc aacctgccga cttaactgcg ctgccagttt cattgccagt
    19501 agcgaatgac cgccaagcgc gaagaaatca gcatcggcat cctgcacgtc acaccccagc
    19561 aacgacgaga atgccgcggc gataatcgtt tcactgcccg ctttcggcgc acgccctggc
    19621 gcttgtgcct tcagttcagg caacggtaag gctttgcgat ccagcttgcc gttggcgcta
    19681 agtggtaact gtggaagttg cagcagaacc accggtacca tatgtggtgg caatgtttca
    19741 cgaagctgcg cctgcaatgc gctggtatcc aacggcaggc ccgattgcga caccagatag
    19801 cccaccaatt gacgcgcatc accaccggtg gcagccgcct ggttaatcac acaggcgtgg
    19861 gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc gatcgatttc gcccagttca
    19921 atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc caccgcgccg
    19981 ttatccagcc agcgggcaac gtctccggta cggtacatcc gttcaccagg agcaaaagga
    20041 tcggcaataa agcggctggc ggtcagatcg ggtcgtccaa gatacccctg cgccagttga
    20101 ataccggtga gatagagatc tcccgccaca cccggcggca ccggatgcat catcgcatca
    20161 agaatacgca ggcccgtgtt ccacaccgga taaccaatcg gcacactgct gccgcgcacc
    20221 tgtgccagtt cctcgccaaa agccggatac cagctgacat ctaccgccgc ttccgtcggg
    20281 ccatatagat tatgcaacgg cgcgccagtt aactgttgcc attcgcggca taaatcagcc
    20341 ggtaaggcct caccactaca gaaaacctgt ttcaacgtcg cgcaactctg gcgagcggtt
    20401 tgcggcgtca gcgaggcaac aaatgccgcc agcatcgacg gcacaaagtg cgtggtcgtt
    20461 acgccatatt cggcaaagaa ttgctgcata gcgagcgggt cgcggtgcgc ttccggttca
    20521 gccatcacca gttttgcccc tgcgataaac ggccagaaaa actcccacac cgagacatca
    20581 aaactgcacg gcgttttttg ggcaacgaca tcttcgcctg taagcggata atgattttgc
    20641 atccaaagca ggcggttgac gatagccgtc tgcccgacca ttaccccttt cggcctgccg
    20701 gtggagccag aggtaaagat gatataagcc gtgtgatgcg gttgtgaaag ttgcagcggc
    20761 gcactgccct gcggtgtaag cggggcgtta tagcaaaggt tggttaaatc tggaacatcg
    20821 gcaaagcgcg gcagttgatc gtcggtggtg attaacagcg acggacgcgc atcttctagc
    20881 atcattttca ggcgatcgtc cggataaccg gtatccagcg gtagccaggc cgcacctgct
    20941 tcaacaatcg catgtagcgc cagggtcaaa aagaccgagc gcggtagcgc caccgctacg
    21001 ctgtcgcccg gtttaacgcc gcgctcacgc agcagattcg ccagcgccac cacctgctca
    21061 cgcatttccc gatagctgaa ctggtaatgc gcatctgcca gcgccggagc atccggagtt
    21121 tttgccgctt gttctgccac cagagcgcta agcgtggttt ctggaatctc aacctgagtg
    21181 gcgttgatct gcgccagctg cgcgtattca cctggcagca taatatcgac atcaccgcac
    21241 aacagcgccg gatctgcggc gaactgggca atcagcattt tcaggcgttc agcatgctgg
    21301 attaacgttg gctcagcgta acgctgttta ttggcgagga tctcaatact caaatcaccg
    21361 tgtacatccg ggaacagggc cagttcaagg tcattaaccg gaccggttgc cagggtatgg
    21421 gtttgcgcct gaacatcagg aatatccagt tggtaatcaa ataccttgat attgagtacc
    21481 ggaccaaaca gcggttcatc acctgccgct cgcccgctgt cacggacaat ttgttcggca
    21541 tcgtaacgtt gatgacgacg catttttttc agttgtgctg ccagtcgggt tgccagttcc
    21601 ggcagcgttt cttgcgccgc aatgtgaata cccaacggca aaacgttgag cacgggtccg
    21661 gtagccgtca gcgccgccga gcccagtcga cgcataaaga taaatccggc ggcgtagtcc
    21721 atacgattgc acaatcgccc cagccacaag gctgccagcg caagggctaa atcggtacgc
    21781 tgcacacctg aaagttgcgt agccagctgg cggaattccc cgtcggtaaa ttccagtttc
    21841 aggcgcagaa tatctgccga ggcgctgcgc cccggtaaag gtgccggaga aagtgacgcg
    21901 ggcggcggca gttgacgacg ctgttctgcc cagaatgccg catcacgctg ccaggcttcg
    21961 ctttcgcggt attgctggta ctcttccact acatcagcga aaggcgtaaa cggcgaagca
    22021 ggcgttggtt cgccacgcag ccatgtgcag taaatattgg cgatctggcg agtaatggcc
    22081 gggaaactga agccatcgac cagcaaatgg tgataacgct gataccagta ccagcggtta
    22141 tccgcaacat gtatcagctg atgaaagacc agtggtttac cgctatcgac gcgcaaatct
    22201 tgttgcaaat ccgccagcat taatgcctgc gcagtaccgt gcggatcaat attggttcgc
    22261 aggtcgataa tttctggcag ttcgaatatc agcgcatcat cgacccactg ccagacttcg
    22321 ccgttatctt ccgtaaaacg catccgcaac gtatctgctt gcgctagtcc gacaaccacc
    22381 gcgcgggcca gtaatggcgc atcaacatct ccagttaact caacgtaatg cgccacgctc
    22441 caggcggagg gtaaatccga cagtttttct gccatccaga tgccgggctg tgcggcgacc
    22501 aaaggtaaat gctggctcat tgtgcctcct gcaactgggt gaaattggcc ggtgtcagag
    22561 tacgccagtg gacttccagc cactgctggc aggacgcctg tgactgcggc tgacacacaa
    22621 tgtcccagcc tgccggtaag gcgcattgtt gcggccacag actgaattgc ccctgcgcat
    22681 tgcgcaaaat gtaaaacgct ccctgcggat catcgaaggg attactgaat gccatattca
    22741 actcctgtca tggaaaagtg gttgccagag gtcgattagc ccctgcatca agccaccgcg
    22801 ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt cttttatggg
    22861 gtgtaattgc gcatacagcg cctgattggc ccgcattatc atcggctcgc gaatacccgc
    22921 ttccagcaca atgcgcagac cttcggcact aacttgacca gctttaagtt gctcaagtaa
    22981 aacaccttct tgctgcccgc cccgatgcgg ccaccagtac gatcctgact ggcttaatac
    23041 acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggaaagtc caccaaaact
    23101 ctgcccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag ctttcaccag
    23161 gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca gttcgtgagc
    23221 gcggtgcgta gtgtcgatag cgtcgatcaa cacatacacg gcgggaggaa gttgctgacg
    23281 atgggtcaac gaagtcagca caggccagac aggcatactc tgcgcccaaa attcgccatc
    23341 gagcaaaact gccagcgggc gttctcctgc tgttgcatcg ccagtggtaa aaatccatac
    23401 acggcgtgaa tttttcaacc gttcactttt ccagataatt tctttggcag gcgtttctgg
    23461 cgcttgcgga caatcccatc ccggttgcag aggcgcttgc ggcatttcga gtgcagaaac
    23521 agcgtgccct cgcccgcctt tccagctttg tggattcagc ggatcggcta ttgcctgggg
    23581 taatagtttt cgccagcctt cacgtaattc gaggcgatcg ggggatggtg cagaaaaaat
    23641 gtcatcgcgt tcggtgggaa taaaacagta gctgccgcgc cagttggcat tgagttgtgt
    23701 cgtccactgc cagacgttgg tgcctgcaat tcgctgcatc gactggggct ggctgttctg
    23761 atggtgatcg gtcacaccag tgatgtagac ccatacgcgc tttatcgtcg agtattcttc
    23821 agaaccttgg ggatcacgcc accagaaagt gacctcaaac atttcgtcat tcagacgctg
    23881 ccattccggg ccatgtttcg actgccacca gctctcactt cctactttta acgccgtcac
    23941 accataaccc catgtttact gtgcaatttt tcattgattg cagaaatata ttgataatat
    24001 tattgataac tatttgcatt tgcaatagcg taatggcgcg ccgtgggaag cgcggacatt
    24061 aattaaccaa ctgcactgcg tgtctttcag gatcaaaggt tttcgcggta gcgggatgcg
    24121 tcgtattgat gacgaccatg cccgacagtt gcaattcgtg gcaaaaatgc aggaataaaa
    24181 caatgaacaa gaagattcat tccctggcct tgttggtcaa tctggggatt tatggggtag
    24241 cgcaggcaca agagccgacc gatactcctg tttcacatga cgatactatt gtcgttaccg
    24301 ccgccgagca gaacttgcag gcacctggcg tttcgaccat caccgcagat gaaatccgca
    24361 aaaacccagt tgcccgcgat gtgtcggaga tcatccgtac catgccaggc gttaacctga
    24421 ccggtaactc caccagtggt cagcgtggta ataaccgcca gattgatatt cgcggcatgg
    24481 gtccggaaaa cacgctgatt ttgattgacg gcaagccagt aagcagccgt aattcggtgc
    24541 gtcagggctg gcgtggtgag cgcgataccc gtggtgatac ctcctgggtg ccacctgaaa
    24601 tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc gcgttatggc aacggcgcgg
    24661 cgggcggcgt ggttaacatc attaccaaaa aaggcagtgg tgagtggcac ggctcctggg
    24721 atgcttattt caatgcgcca gaacataaag aggaaggtgc caccaaacgt accaacttca
    24781 gcctgaccgg tccgctgggc gacgaattca gcttccgctt gtatggcaac ctcgacaaaa
    24841 cccaggctga cgcgtgggat atcaaccagg gccatcagtc cgcgcgtgcc ggaacgtatg
    24901 tcacgacgtt accagccggg cgcgaagggg tgatcaacaa agatattaat ggcgtggtgc
    24961 gctgggactt cgcaccattg caatcgctgg aactggaagc gggttacagc cgccagggta
    25021 acctgtatgc gggcgatacc cagaacacca actctgacgc ttacactcgc tcgaaatatg
    25081 gcgatgaaac caaccgcctg tatcgccaga actacgcgct gacctggaac ggtggctggg
    25141 ataacggcgt gaccaccagc aactgggtgc agtatgaaca cacccgtaac tcgcgtattc
    25201 cggaaggtct ggcgggcggt accgaaggga aatttaacga aaaagcgaca caggatttcg
    25261 tcgatatcga tcttgatgac gtgatgctgc acagcgaagt taacctgccg attgatttcc
    25321 tcgttaacca gacgctgacg ctgggtacgg agtggaatca gcaacggatg aaggacttga
    25381 gttccaatac ccaggcgctg accggggcga acaccggcgg cgctattgat ggtgtgagtg
    25441 ccaccgaccg tagcccgtat tcaaaagcag aaattttctc gctgtttgcc gaaaacaaca
    25501 tggagctgac tgacagcacc atcgtaacgc cggggctgcg tttcgatcat cacagtattg
    25561 tcggcaataa ctggagcccg gcgctgaaca tatcgcaagg tttaggcgat gacttcacgc
    25621 tcaaaatggg cattgcccgt gcttataaag caccgagcct gtaccagact aacccgaact
    25681 acattctcta cagtaaaggt cagggttgct atgccagcgc gggcggctgc tatctgcaag
    25741 gtaacgatga cctgaaagca gaaaccagca tcaacaaaga gattggtctg gagttcaaac
    25801 gcgacggctg gctggcgggc gtcacctggt tccgtaacga ttatcgcaat aagattgaag
    25861 cgggctatgt ggctgtagga caaaacgcag tcggcaccga tctctatcag tgggataacg
    25921 tgccgaaagc agtggttgaa ggtctggaag gatcgttaaa cgtaccggtt agcgaaacgg
    25981 tgatgtggac caataacatc acttatatgc tgaagagtaa aaacaaaacc acgggcgacc
    26041 gtttgtcgat catcccggag tatacgttga actcaacgct gagctggcag gcacgagaag
    26101 atttgtcgat gcaaacgacc ttcacctggt acggtaagca gcagccgaag aagtacaact
    26161 ataaaggtca gccagcggtt ggcccggaaa ccaaagaaat cagtccttac agcattgtgg
    26221 gtctgagcgc gacctgggat gtgacgaaga atgtcagtct gaccggcggc gtggacaacc
    26281 tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac cacgggcgat ctggcagggg
    26341 ccaactatat cgcgggtgcc ggggcgtata cctataacga gccgggacgt acgtggtaca
    26401 tgagcgtaaa cactcacttc tgatgctaac gtcagattgt tgacaaagtg cgcgtcgttc
    26461 atgccggatg cggcgtgaac gccttatccg gcctacaaaa atcttgccaa ttcaatatat
    26521 tgcaggacca tgtaggcctg ataagcgtag cgcatcaggc agtttagcgt ttgtcatcag
    26581 tctctaatat ggtcgacatg aaaactacgc atacctccct cccctttgcc ggacatacgc
    26641 tgcattttgt tgagttcgat ccggcgaatt tttgtgagca ggatttactc tggctgccgc
    26701 attacgcgca actgcaacac gctggacgga agcgcaaagc cgaacattta gccgggcgga
    26761 tcgctgcaat ttatgcgctg cgggaatatg gctataaatg tgtgcccgca atcggcgagc
    26821 tacgccaacc ggtgtggcct gcggaggtat acggcagtat tagccactgc ggggctacgg
    26881 cattggccgt ggtttcccgt caaccgattg gcgttgatat cgaagaaatt ttttctgcgc
    26941 aaacagcgac cgaattgaca gacaacatta ttacaccagc agaacacgag cgactcgcag
    27001 actgcggttt agccttttct ctggcgctga cactggcatt ttccgccaaa gagagcgcat
    27061 ttaaggcaag tgagatccaa actgatgcag gttttctgga ctatcagata attagctgga
    27121 ataaacagca ggtcatcatt catcgtgaga atgagatgtt tgctgtgcac tggcagataa
    27181 aagaaaaaat agtcataact ctgtgccaac acgattaatt gacaacatct ggtacgattc
    27241 gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac cactctgacc
    27301 tgacaaccgg atatgcgggg attgctcccc gcacaacggc tacttcttcg gttcgtaagc
    27361 gagaacagcc ttaaactcaa tattacgttc ctttacggtg aactcacaca gcgagtctcc
    27421 gaccagaagc gtaaatccca gtaccgtcaa acacagcact atgactgcca caagggcata
    27481 tttcgtcagc atgtttatat tgcctccatg cgaagaaggg actaccatcc gagttgttga
    27541 ggctcggagt ggcagcccca ggattgatag aaatatctcc tggggcttca tctttccaga
    27601 cctcagagtt aacctgaaac cggaaagctt caggcacccg ccgttatctt acccttcctc
    27661 aacaaaatag ctgtttattt atacagtgaa tcctgtttcg gacttctgtc ggcaaaactc
    27721 atcattgctc ttaaaacgaa atcgcccgca gccatcactg accgcgggcg aaagtgtaaa
    27781 gcaggtgcct taccatcctg acctgacaac cggatatgcg gggattgctc cccgcacaac
    27841 ggctacttct tcggttcgta agcgagaaca accttaaact caatattacg ttcctttacg
    27901 gtgaactcac acagcgaatc cccgaccaga agcgtaaatc ccagcaccgt taaacacagc
    27961 actatgactg ccgcaagggc atatttcgtc agcatgttta tattgcctcc atgcgaagaa
    28021 gggactacca tccaaattgt tcaggtttgg gtaggtagcc ccagggttga tagaaatatc
    28081 gtcctggggc ttcatctttc cagacctcag agtaaacctg aaaccggaaa gcttcaggca
    28141 cccgcagtta tcttaccctt gcttagcaaa atagctgttt atttatacag taagtcctgt
    28201 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
    28261 tcaatcgtca aaattcttcg ccaccagcca gccgtgccat aacttgtcac attgcaaaag
    28321 tagccaaatt gcttcagtca ccatcaccaa aaaaagaaac attccagaac ttccgcatct
    28381 cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacttaagag aaacctatgc
    28441 cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg gaaatgcagg
    28501 tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt gacgcggtta
    28561 aaaatgagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg ctggagctgg
    28621 cgacggatgt ttgccgtgat atcaaccagg ctgccgggca gttttcagcg atgcagaaag
    28681 tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc acgcacccgt
    28741 ttcagaaatg gcagcgtcag gaggtatgcg acaacgaacg ctatcaacga acgctggaaa
    28801 actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat gttggctgcg
    28861 ccagtggcga tgacgccatt tatttgctgc acggcctgtc acggtttgtg ccgcacttca
    28921 tcgccctttc cgccgcgtcg ccgtatatgc agggaacgga tacgcgtttt gcctcctcac
    28981 gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc agtaactggc
    29041 aacaatttga agccctgttt cgctgcctga gttacaccac gatgatcgac agcattaaag
    29101 atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt cgggtgatgg
    29161 ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag gccaccgccc
    29221 actggttact gacggaacgc ccgtttaaac atcaggagaa agattacctg ctgtataaat
    29281 tcaaccgttt ccaggcctgc cgctatgggc tggaaggcgt cattaccgat ccgcacactg
    29341 gcgatcgtcg accgctaacg gaagatacct tgcgattgct ggaaaaaatc gccccttccg
    29401 cacataaaat gggtgcatcg agcgcgattg aggccctgca tcgccaggtc gtcagcggtc
    29461 tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg attgggctgg
    29521 tgaaaaagca ttgtgagatc tgggccggtg actaagtagg gaattgcctt acagaagaat
    29581 atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc ctttagaaac
    29641 cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc
    29701 ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc tgatggatct
    29761 tagccagctt tacactttat tgttttgtct gtggttttta gtgctgggcg ctattgagta
    29821 ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa cgtcgattat
    29881 cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg tttatggata
    29941 agcaatcact gcacgaaacg gcgaaacgcc tggcccttga gttacccttt gtcgagcttt
    30001 gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt tttatgctgt
    30061 cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa aaatccctgt
    30121 taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa aagcactgga
    30181 tttcggtgta tcccggcgag gaaatctccg aagcgttaat tcgcgatctg atcaacgatt
    30241 cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg cgtccaggct
    30301 aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac cttgtaatct
    30361 gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct gtcgccttaa
    30421 agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa caggccgagc
    30481 agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg tggcatttta
    30541 ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc ggtaattatg
    30601 tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg
    30661 cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat ggccgctttg
    30721 ccacaccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct gatatgcacc
    30781 gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc aacgtcggta
    30841 acttcctgct cggcgtggcg gcgctgggtc tggacgcggt acccatcgaa ggttttgacg
    30901 ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt ctggtggttg
    30961 ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa tctcgtctgc
    31021 cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg cccggctatt
    31081 tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata ggctgctgtt
    31141 gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag cgggtattga
    31201 gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga ccgccgtcgt
    31261 ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg ccatcgccag
    31321 ttcacggctt tggttgcaaa tcattaccca gaataaactc ttccaccgtt tagcttttac
    31381 cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag gcaccgaagc
    31441 ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc tgctttcagt
    31501 cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg catctcagtt
    31561 accgctgaaa gggatatttc aggggattaa actgatcggc gcaatcctgg tcggcatttt
    31621 gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc ttggtgcaat
    31681 ggctgccgtg ctgatgttgg tttttaaaga tccgattctt ggtctggtgg caggtattca
    31741 gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga aatacggcgc
    31801 ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact gggacaatac
    31861 cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact ggagcgggat
    31921 gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca ccagtattcg
    31981 ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg cacttgttaa aaccttattt
    32041 aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta cggagtcgat
    32101 attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga acgaatatct
    32161 gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac tggctccagg
    32221 tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt ggctggaata
    32281 tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag agtttggtct
    32341 gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg catttaagca
    32401 gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt gtcggatgta
    32461 gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa tacaatccac
    32521 accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag cagcaaaatc
    32581 atgccgagga aggcaatgca aagatagttg ccgaacggat agagcagcgc cttaaactgt
    32641 gtttcacgcc cctgacgtcg catcgctgca cgaaaacgca gatgcgccag acagatcata
    32701 atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa cgctttttgc
    32761 ggcagcagat agttgattaa taccaccagc gaagtgatcg ctccggaaag catcagcgag
    32821 ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt accctgcaca
    32881 gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac tgacagcgat
    32941 gctaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag attatggaaa
    33001 atcatcacaa acgggctact gttggatttc acttccaccc acggatagag cgccagtaaa
    33061 accaccagtg aaccgatgta aaacagcagg atgcgataca ccacctgatt taccgctttt
    33121 ggaatgcttt tttccggatc gcgcgcttca gcggcagtaa tcccaatcag ctccagaccg
    33181 ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc ggtggcgaag
    33241 aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg accagaaaac
    33301 agcagccaca ggccaaagcc gatcataccg atgatcgcca gcactttaat cagcgcaaac
    33361 cagaactcgg tttcaccata taagcgcacg ttcaccaggt taacggcgtt gatgataata
    33421 aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg catatagatg
    33481 cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta gttccagcca
    33541 gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa tgaaccggat
    33601 accggctcct caaccaccat ttcgccaagc tggcgcataa tcaggaaagc gatgatcccg
    33661 gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg gccaatgcca
    33721 agaaacagac cagtaccaat tgcgccaccc aacgcaatca gttgaatatg acggttatgt
    33781 aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac ggttgacgcg
    33841 tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta acatttagtg
    33901 cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt tatttccgta
    33961 cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac agcgacagca
    34021 aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc acctctgaac
    34081 cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc accgtcatcg
    34141 ctttcgggcg cacgcgcagg accgcgccgt gatataacgc ctcatccagc ttctgctcgc
    34201 tgaatgtttg tggattattc aacgacggca cggcctctat ggcgtgacgt aaatacatca
    34261 gcatcaccac gccaaattcg gcggcgaccc cggcgagggc gataaagcca gtgcccgtcg
    34321 ccacggaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc agcgcaaacg
    34381 gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaacgccaga tacaacagca
    34441 cgaagataat catcaacgtc atcggcacca tgagtttaag cttatggttg gcgcgctcca
    34501 gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc actttttcag
    34561 ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg gcatcgatat
    34621 aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg gtagagactt
    34681 taatgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg atcggcagct
    34741 ggcgcagtgc ctgcggacta tcgcgccagc tttgcggata acgcagatta attggataac
    34801 gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa gtcacaaaca
    34861 actgcacatc cgccaccgtc ataccgtaac gtgcggcttt ttcacggtta atctcaacgt
    34921 tgatatagcg cccaccttcc agccgctcgg caagcgcaga agctacgcct ggcaccgttc
    34981 gcgctacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca gtgccggaaa
    35041 ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg ttacgaattg
    35101 gcggcaccca cagattcgcc agccccggca gccgcacggt gttatccagt tcctcaatga
    35161 ttttgtccat cgtcatgcct ggccgccact gctcctgcgg cttaagctgg atggtcgttt
    35221 ctaccatctc cagcggagca gaatcggtgg cggtttccgc tttcccggtt ttgccaaata
    35281 cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc atactcgccg
    35341 cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg ccttcattga
    35401 tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc agcaccgaaa
    35461 gcgccgccac cagcagcgtg gttttcggcc agtgcagtac tttcagcaac agcggatgat
    35521 aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt ttgccacgga
    35581 tccagtagcc catcaggatc gggatcacta cgatcgccag cagcgccgca cccgccatcg
    35641 catacgtttt ggtgaacgcc aacgggccaa acagacgccc ttcctgccct tccagggtga
    35701 agatcgggat aaacgacaac gtgataatca gcagactgat aaatagcgcc ggccccactt
    35761 caacagacgc atcggtgatc acctgccagc gcgttttatt atccagcgtg gcgtcaggat
    35821 gctggtgctg ccactcttcc agccgtttat gtgcattttc gatcatgacg atagcggcat
    35881 cgaccatcgc cccgacggca atcgcaatgc cacccaacga cataatattg gcattcagtc
    35941 cctggaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa ataatcgcca
    36001 ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc acaataaact
    36061 cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg ctgcgatcgt
    36121 atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc agtttgtcct
    36181 tcacggcggc gatcacttct cgggcgtttt tgccggatcg caggatcacc accccgcccg
    36241 ccacttcgcc ttcgccgttt agttcggcaa tgccccggcg catctccggg ccaatctgga
    36301 ccttcgcaac atcgcgcaga taaacgggca cgccattttc actggctttt aaaacgatgt
    36361 gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat tccgcttccg
    36421 ccagttcgat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg ctttttactt
    36481 cggcgagact gatgccatac tgcgccaggc gctggggatc gataaccacc tgatactctt
    36541 tcaccacacc gcccaccgac gccacttccg caacgtcagg gatggttttc agctcatatt
    36601 tgagaaacca gtcctgtaat gagcgtaaat cggccagatc gtgcttatcg ctgcgatcca
    36661 ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggccccagc tcggcgctga
    36721 ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc gaacgcgccc
    36781 agtacggatc ggtgccatct tcgaaaatga catacacata agagtcgcca aactgtgaga
    36841 aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc ggataagtca
    36901 cctgattttc aacgatttgt ggtgcctgac cgggatagct ggttttaata ataacctgca
    36961 catcggagag atccggcagc gcatccactg gcgtattaat gatggtccag gtgccccaga
    37021 tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag cgacgaataa
    37081 tccattcaat cattggttat tccctcaatg cgcatgggta gcactttcag agcgcatccg
    37141 ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc tggaaaccac
    37201 cttttcacct tccgccagac cagagcgtaa tgcggtgacg ccttgcgatg cctggaaaac
    37261 agcaacgcgt ttcggtacaa agcgcccgtc ggcatcaacg gtaatcaccc gctgttcgct
    37321 gccggtatca atcagcgctt gtgacggaat gagcagcatc ggttcgctgg cggtgttgag
    37381 ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga cttccagacg
    37441 cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt tgcggatggt
    37501 gagtgtttta tccggtcgcg ccggaacggt gagggtaaac tgcgaggcat ctttcaccag
    37561 ccaggcgata gactccggga tcgcagcagt gacccacacc gggtccatac cctgaatttt
    37621 cgctaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg cggtgatcac
    37681 gccatcaatg ggcgctttga gcgtaaagcg agtctggatt ttttgcgtgg cgatcaggcg
    37741 gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc cttcagtctg
    37801 ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt ccacccagtc
    37861 aggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca cggtaagcgg
    37921 atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact gatactcgtt
    37981 gtaactgaca ttcgccggga aactctgggc aaaagtcagc ggcccgcgcg tgacggtagc
    38041 cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag acgcagaact
    38101 ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt tacctggttt
    38161 atcgaaccgc gtattggggt acatcgggtc gtaccagaat aagattttac gttctgcggt
    38221 cgacgttttt tctgcgggtg gttcagcctt tgcaaaccag gtaaaacctg ccgcagaaat
    38281 aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct cattaaacct
    38341 gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct ggacaaaatt
    38401 aaacgccact ttgtcgccag ttttaatttc actcattttc gtctgcggag tgatggtaaa
    38461 gcgcatggtc atctccggcc agttcacggc agcaatcgga tcgtgatgga tggtgatttt
    38521 tttgctttcc agatcaatac cctttaccac gccagtggcg ctaataacct gcggttgtac
    38581 ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa taacggtaaa
    38641 cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg agttaaaaat
    38701 tattgctgcc aaccgccccc taaggcggta tacaaagaaa tttcgttaac ctgacgggca
    38761 taattcaggt cgagtaaagt ttgtcgggtt gcaaataaag aacgctcggc atccagcact
    38821 tccagataac ttaccgcgcc gtgctgatat aatgcccgcg cccgttgcaa agtaatttgc
    38881 agcgacgcca gataacgctg ctgggcgctg atttgatcgt tcaggctttg acgtaatgcc
    38941 agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt cactaccgac
    39001 tgctgctggc gaatttcggc gatatccaga ttggcttggt tgcgtccggc attaaaaatg
    39061 gggatctcaa ttttaggaat aaaattccac atcccgctgc tagcgttaaa taatgaagac
    39121 agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa aaatgccgca
    39181 cgcgcagcgc caatattggc attagccgcc attaacgcgt gttcagcttc cataatatcc
    39241 gggcgctgca ataagatttg cgacgacaat cccgccggta atttaacgct ttgcaggctg
    39301 tcgctgttta ctgtctgcgc ttgcggcagc ttgccgtagc ttcccaataa cagttgcaat
    39361 gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcgctgcg ggtactttct
    39421 atcaccccgc gagcctgttc cagcgccaga acattactgc tacctgtcaa cagttgtttt
    39481 tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat ttgcaattgc
    39541 gcatacgcca gttgctgatt gaaatagctt tgcgcgacat tagaaaccag cagaatatgc
    39601 accgcgcgct gagcttcctc agtggctaaa taattttgtc gctcggcttc gctcatgttc
    39661 tttaagcgac cgaaaaaatc gaggtcaaag ctggcgttaa ggccggtcga aaactcccgt
    39721 gtcgtggcgg tattcccttt aagattaccg ctccagctgc cgctgccctc gccattgagc
    39781 tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gtgcctcctg cactttcagc
    39841 gtcgccatgc gcaaatcccg attattcacc agcgcctcgc taatcagcgt cttcacctga
    39901 ttatcaacaa aaaaggtgcg ccagcccgcg ttctgatagt tatctgctgc gttaaccagg
    39961 ccgttctggc tgagtgagaa ctgctgcggc acgggcattg ccggacgctg ataatccggt
    40021 gccagtgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt acaaggagac
    40081 ataggctcat aatttctggt gattttatgc cgccaacttt actcgccagg ctctgatttt
    40141 ccggtgacag aaaaatgaca aaattgtcat tttgccaata agcgattgcc atctgatccc
    40201 gctactctag aattgcccgg gcaacatgcg gaggaaatat gaaactgttg attgtcgaag
    40261 atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt tttgtggtcg
    40321 atttggccga caacgggttg aatggctacc atctggcgat gaccggtgat tatgatctga
    40381 taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc atgttgcgtt
    40441 ccgccaataa agggatgccg attctgttgc ttaccgcgct tggcaccatt gaacatcgcg
    40501 tcaaggggct ggagttgggg gcggatgact atctggtgaa gccgttcgct tttgctgaac
    40561 tgctggcgcg ggtgcgcact ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt
    40621 ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc
    40681 gcatcactct gaccagtaaa gagtttactc tgctggagtt tttcctccgc catcagggcg
    40741 aagtgctgcc ccgctcgctt atcgcctcgc aggtgtggga catgaatttc gacagcgaca
    40801 ctaacgccat tgatgtagcg gtgaagcggc tgcgcggcaa aatcgacaac gactttgagc
    40861 caaagctaat tcagaccgtg cgcggcgtgg gttacatgct tgaggtgccg gatggtcagt
    40921 aagccatttc agcgcccgtt ttcgctggca acccgcctga ccttttttat cagcctggcc
    40981 accatcgcag cgtttttcgc ctttgcatgg atcatgatcc actcagtaaa agtgcatttt
    41041 gccgagcagg atattaatga tttaaaagaa attagcgcca cgcttgaacg ggtacttaat
    41101 catcctgacg agacgcaagc ccgacgctta atgacgctgg aagatatcgt cagtggttat
    41161 tccaacgtgt tgatctccct ggcagatagc catggtaaaa cggtgtatca ctcccccggt
    41221 gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc gcggggcggc
    41281 gaggtgtatc tcctttccgg cccgacgatt atgatgccag ggcatggtca cgggcatatg
    41341 gaacacagca actggcggat gattaacttg tcggttggcc cgttggtgga cggcaaaccg
    41401 atttatacgc tctacatcgc gctttcgatc gattttcatc ttcattacat aaatgatttg
    41461 atgaataaac ttattatgac cgcatcgatc atcagcatcc tgatcgtctt tatcgtgcta
    41521 ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatt
    41581 acctcgaaag atctcgacgt tcgccttgac ccgcagaccg tgcccattga gctggaacag
    41641 ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac ccgccagtcc
    41701 aatttctcag cggatatcgc tcacgaaatt cgcacgccga ttaccaatct catcacgcaa
    41761 acggaaatcg ccctcagcca gtctcgcagc cagaaggagc tggaagatgt gctctactct
    41821 aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt tctcgctcag
    41881 gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga tgaagtcggc
    41941 aaagtgttcg attttttcga ggcgttagcg gaagatcgcg gcgtggagtt gcgatttgtt
    42001 ggcgacgaat gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt aagcaacctg
    42061 ctttctaatg ccctgcgtta tacgccgccc ggagaggcaa ttgtagtgcg ctgccagacg
    42121 gtcgatcacc aggtgcaagt taccgtcgaa aaccccggta cgcccattgc gcccgagcac
    42181 ttaccgcgat tgtttgaccg tttctaccgc gttgaccctt cccgccagcg aaaaggcgaa
    42241 ggtagcggca ttgggctggc gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt
    42301 gcagtaacgt cagatgcgcg ggggacaagg tttgtgatta tgctgccgga gagagagtga
    42361 ttattaagtt caaattcagg gcgtggtgca tcacctcctg gtattgttga tcaacaaatt
    42421 agagaaaaac ttattaaaca tatgaaatat cgaaggcttt tatttttcaa agtttcacga
    42481 gatgtttgta tatgaaactt agttaaatct gtgtttcttc caattgtaga tcacaatgcc
    42541 ctttctaaat tttcatcgtt a
//
LOCUS       DAZFWP020000018        40900 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFWP020000018 DAZFWP020000000
VERSION     DAZFWP020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.018"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            143..478
                     /gene="eutS"
                     /locus_tag="ACHK0Z_RS04635"
                     /old_locus_tag="pgaptmp_000927"
     CDS             143..478
                     /gene="eutS"
                     /locus_tag="ACHK0Z_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU4500992.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            491..970
                     /gene="eutP"
                     /locus_tag="ACHK0Z_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             491..970
                     /gene="eutP"
                     /locus_tag="ACHK0Z_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU4500993.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLMDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            945..1646
                     /gene="eutQ"
                     /locus_tag="ACHK0Z_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             945..1646
                     /gene="eutQ"
                     /locus_tag="ACHK0Z_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU4500994.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1643..2446
                     /gene="eutT"
                     /locus_tag="ACHK0Z_RS04650"
                     /old_locus_tag="pgaptmp_000930"
     CDS             1643..2446
                     /gene="eutT"
                     /locus_tag="ACHK0Z_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU4500995.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2443..3459
                     /gene="pta"
                     /locus_tag="ACHK0Z_RS04655"
                     /old_locus_tag="pgaptmp_000931"
     CDS             2443..3459
                     /gene="pta"
                     /locus_tag="ACHK0Z_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4500996.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     PLGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3498..3791
                     /gene="eutM"
                     /locus_tag="ACHK0Z_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             3498..3791
                     /gene="eutM"
                     /locus_tag="ACHK0Z_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU4500997.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3898..4185
                     /gene="eutN"
                     /locus_tag="ACHK0Z_RS04665"
                     /old_locus_tag="pgaptmp_000933"
     CDS             3898..4185
                     /gene="eutN"
                     /locus_tag="ACHK0Z_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU4500998.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4197..5600
                     /locus_tag="ACHK0Z_RS04670"
                     /old_locus_tag="pgaptmp_000934"
     CDS             4197..5600
                     /locus_tag="ACHK0Z_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4500999.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5611..6447
                     /gene="eutJ"
                     /locus_tag="ACHK0Z_RS04675"
                     /old_locus_tag="pgaptmp_000935"
     CDS             5611..6447
                     /gene="eutJ"
                     /locus_tag="ACHK0Z_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU4501000.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6437..7624
                     /gene="eutG"
                     /locus_tag="ACHK0Z_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             6437..7624
                     /gene="eutG"
                     /locus_tag="ACHK0Z_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU4501001.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7741..8967
                     /gene="eutH"
                     /locus_tag="ACHK0Z_RS04685"
                     /old_locus_tag="pgaptmp_000937"
     CDS             7741..8967
                     /gene="eutH"
                     /locus_tag="ACHK0Z_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU4501002.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8964..10367
                     /gene="eutA"
                     /locus_tag="ACHK0Z_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             8964..10367
                     /gene="eutA"
                     /locus_tag="ACHK0Z_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU4501003.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10379..11740
                     /gene="eutB"
                     /locus_tag="ACHK0Z_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             10379..11740
                     /gene="eutB"
                     /locus_tag="ACHK0Z_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU4501004.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11761..12648
                     /gene="eutC"
                     /locus_tag="ACHK0Z_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             11761..12648
                     /gene="eutC"
                     /locus_tag="ACHK0Z_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU4501005.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12658..13317
                     /gene="eutL"
                     /locus_tag="ACHK0Z_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             12658..13317
                     /gene="eutL"
                     /locus_tag="ACHK0Z_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU4501006.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13330..13830
                     /gene="eutK"
                     /locus_tag="ACHK0Z_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             13330..13830
                     /gene="eutK"
                     /locus_tag="ACHK0Z_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU4501007.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13876..14928
                     /gene="eutR"
                     /locus_tag="ACHK0Z_RS04715"
                     /old_locus_tag="pgaptmp_000943"
     CDS             13876..14928
                     /gene="eutR"
                     /locus_tag="ACHK0Z_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU4501008.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14934..15833)
                     /gene="hemF"
                     /locus_tag="ACHK0Z_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             complement(14934..15833)
                     /gene="hemF"
                     /locus_tag="ACHK0Z_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU4501009.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15837..16706)
                     /gene="amiA"
                     /locus_tag="ACHK0Z_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             complement(15837..16706)
                     /gene="amiA"
                     /locus_tag="ACHK0Z_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU4501010.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16920..17345
                     /locus_tag="ACHK0Z_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             16920..17345
                     /locus_tag="ACHK0Z_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU4501011.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17332..17781
                     /locus_tag="ACHK0Z_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             17332..17781
                     /locus_tag="ACHK0Z_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU4501012.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            17842..18417
                     /locus_tag="ACHK0Z_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             17842..18417
                     /locus_tag="ACHK0Z_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU4501013.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18513..19412
                     /gene="yfeX"
                     /locus_tag="ACHK0Z_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             18513..19412
                     /gene="yfeX"
                     /locus_tag="ACHK0Z_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU4501014.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19470..20771)
                     /gene="pbp4b"
                     /locus_tag="ACHK0Z_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             complement(19470..20771)
                     /gene="pbp4b"
                     /locus_tag="ACHK0Z_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU4501015.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(20776..22200)
                     /gene="murP"
                     /locus_tag="ACHK0Z_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             complement(20776..22200)
                     /gene="murP"
                     /locus_tag="ACHK0Z_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU4501016.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22204..23100)
                     /gene="murQ"
                     /locus_tag="ACHK0Z_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             complement(22204..23100)
                     /gene="murQ"
                     /locus_tag="ACHK0Z_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU4501017.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23264..24121
                     /gene="murR"
                     /locus_tag="ACHK0Z_RS04765"
                     /old_locus_tag="pgaptmp_000953"
     CDS             23264..24121
                     /gene="murR"
                     /locus_tag="ACHK0Z_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU4501018.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24250..25041
                     /gene="ucpA"
                     /locus_tag="ACHK0Z_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     CDS             24250..25041
                     /gene="ucpA"
                     /locus_tag="ACHK0Z_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU4501019.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25212..26228
                     /gene="cysP"
                     /locus_tag="ACHK0Z_RS04775"
                     /old_locus_tag="pgaptmp_000955"
     CDS             25212..26228
                     /gene="cysP"
                     /locus_tag="ACHK0Z_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU4501020.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26228..27061
                     /gene="cysT"
                     /locus_tag="ACHK0Z_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             26228..27061
                     /gene="cysT"
                     /locus_tag="ACHK0Z_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU4501021.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27061..27936
                     /gene="cysW"
                     /locus_tag="ACHK0Z_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             27061..27936
                     /gene="cysW"
                     /locus_tag="ACHK0Z_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU4501022.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27926..29023
                     /gene="cysA"
                     /locus_tag="ACHK0Z_RS04790"
                     /old_locus_tag="pgaptmp_000958"
     CDS             27926..29023
                     /gene="cysA"
                     /locus_tag="ACHK0Z_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU4501023.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29157..30068
                     /gene="cysM"
                     /locus_tag="ACHK0Z_RS04795"
                     /old_locus_tag="pgaptmp_000959"
     CDS             29157..30068
                     /gene="cysM"
                     /locus_tag="ACHK0Z_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU4501024.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30071..30439)
                     /locus_tag="ACHK0Z_RS04800"
                     /old_locus_tag="pgaptmp_000960"
     CDS             complement(30071..30439)
                     /locus_tag="ACHK0Z_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGU4501025.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30544..31395
                     /gene="pdxK"
                     /locus_tag="ACHK0Z_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /gene_synonym="thiJ"
     CDS             30544..31395
                     /gene="pdxK"
                     /locus_tag="ACHK0Z_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU4501026.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31437..31946)
                     /gene="crr"
                     /locus_tag="ACHK0Z_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             complement(31437..31946)
                     /gene="crr"
                     /locus_tag="ACHK0Z_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU4501027.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31987..33714)
                     /gene="ptsI"
                     /locus_tag="ACHK0Z_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             complement(31987..33714)
                     /gene="ptsI"
                     /locus_tag="ACHK0Z_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU4501028.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33759..34016)
                     /gene="ptsH"
                     /locus_tag="ACHK0Z_RS04820"
                     /old_locus_tag="pgaptmp_000964"
     CDS             complement(33759..34016)
                     /gene="ptsH"
                     /locus_tag="ACHK0Z_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU4501029.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34400..35371)
                     /gene="cysK"
                     /locus_tag="ACHK0Z_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             complement(34400..35371)
                     /gene="cysK"
                     /locus_tag="ACHK0Z_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU4501030.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35556..36317)
                     /gene="cysZ"
                     /locus_tag="ACHK0Z_RS04830"
                     /old_locus_tag="pgaptmp_000966"
     CDS             complement(35556..36317)
                     /gene="cysZ"
                     /locus_tag="ACHK0Z_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU4501031.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36547..37533
                     /gene="zipA"
                     /locus_tag="ACHK0Z_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             36547..37533
                     /gene="zipA"
                     /locus_tag="ACHK0Z_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU4501032.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37604..39619
                     /gene="ligA"
                     /locus_tag="ACHK0Z_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             37604..39619
                     /gene="ligA"
                     /locus_tag="ACHK0Z_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU4501033.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39621..39839
                     /locus_tag="ACHK0Z_RS04845"
                     /old_locus_tag="pgaptmp_000969"
     CDS             39621..39839
                     /locus_tag="ACHK0Z_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU4501034.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39836..40834)
                     /locus_tag="ACHK0Z_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             complement(39836..40834)
                     /locus_tag="ACHK0Z_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU4501035.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
BASE COUNT         9523 a        10508 c        11495 g         9374 t
ORIGIN      
        1 atatgaaagt ttatgcacca cagcgaatat ctctccattc ttagtgatct acctcacctt
       61 ttaaacgcgc ttgccgaatt ttgttattta ctctgacgaa aaattgtcac gatacacgaa
      121 agtttttcac aggcggcgac tcatggataa agaacgcatc attcaggaat ttgtgcccgg
      181 caaacaggtc acactggcgc atctcatcgc gcatcccggt gaggaactgg cgaagaaaat
      241 cggcgttccg gatgcgggcg caattggcat tatgacgcta actcccggcg aaacggcgat
      301 gatcgccggt gatttagcgc tgaaagccgc tgatgtgcat atcggttttc tcgaccgctt
      361 cagcggtgca ttggtgattt atggctcggt gggcgcagta gaagaagcgt tatcacaaac
      421 cgtcagcggt ttaggtcgct tattaaatta tacgctttgt gaaatgacca aaagttaata
      481 cgaggcattc atgaaacgaa ttgcgtttgt gggtaccgtc ggggcaggaa aaacaacgtt
      541 atttaatgcg ttgcagggga attataccct cgccagaaaa acacaggccg tggaatttaa
      601 tgataagggc gatattgata cgccgggtga atattttagc catccccgct ggtatcacgc
      661 cttaattacc acgctacagg atgtagacat gctgatctat gtccatggcg caaatgaccc
      721 ggaaagtcgg ttgcctgccg ggttgatgga tattggcgtc agcaaacggc aaatcgccgt
      781 catcagtaaa acggacatgc cagatgctga cgtcgccgca acgcgaaagt tattgcttga
      841 gacgggtttc gaagagccga ttttcgagct aaacagccac gacccgcaaa gcgtgcagca
      901 actggtggat tatctggcat ctctcaccaa acaggaggaa gcaggtgaaa aaactcatca
      961 cagcgaatga tattcgtgaa gcacacgcgc gcggcgaaca ggcaatgtcc gtggttctgc
     1021 gcgccagcat tattaccccg gaagcccgtg aagtggcgga tctactgggt tttaccatca
     1081 ccgaatgtga tgaatcgatc ccggtaacgg cgtccgtgcc cgccagcgtg ccagccgaca
     1141 aaaccgaaag ccagcgcatc cgcgaaacca tcattgccca gctgccggaa ggccagttta
     1201 ccgaaagcct ggtcgcacag ctgatggaaa aagtgatgaa ggaaaaacag tcgctggagc
     1261 agggcgcaat gcagccgagc ttcaaatcgg tgaccggcaa aggcggcatc aaagtgattg
     1321 atggcagcag cgtcaagttt ggtcgtttcg acggcgcaga gccgcattgc gtgggcttga
     1381 ctgatcttgt gaccggagac gacggcagca gcatggccgc tggttttatg cagtgggaga
     1441 acgcattctt cccgtggacc ctgaactacg acgaaatcga catggtgctg gaaggggagc
     1501 tgcatgttcg ccacgaaggc gaaaccatga tcgccaaagc gggtgacgtg atgtttatcc
     1561 cgaaaggctc cagtatcgaa tttggtacga catccagcgt gaaattcctg tatgtcgcct
     1621 ggccggctaa ctggcaatcc ctatgaaaga tttcatcacc gaagcatggc taagagcgaa
     1681 ccatacgctc agcgaaggcg cagagatcca tctccctgcg gacagtcgcc tgacgccctc
     1741 tgcccgggag ttactggaaa gccgccatct gcgcatcaag tttattgacg agcagggccg
     1801 cctgtttgtt gacgatgaac agcagcagcc gcagcccgtt catgggctga ccagtagcga
     1861 tgaacatccg caggcgtgct gcgaactgtg ccgccagccg gtggcaaaaa agccggatac
     1921 gctgactcac ctgtcggcgg aaaaaatggt cgccaaaagc gatccgcgtc tgggttttcg
     1981 cgcggttctc gacagcacca ttgcgctggc agtgtggctg caaattgaac tggcggaacc
     2041 gtggcagccg tggctggcgg atatccgttc gcgtcttggc aacattatgc gcgccgatgc
     2101 gctgggtgaa ccgctgggct gccaggcgat tgtcgggctt tctgacgaag atctgcatcg
     2161 actttctcac cagccgctgc gctatctcga ccacgatcat ctggtgccgg aagccagcca
     2221 cgggcgcgat gcggcgttgc tcaatctgct acgtaccaaa gtgcgggaaa ccgaaaccgt
     2281 agcggcgcag gtgtttatca cccgcagctt tgaagtatta cgcccggaca ttttgcaggc
     2341 gctgaaccgc ctttccagca cggtctacgt gatgatgatc ctgagcgtaa cgaagcagcc
     2401 gctaacggtg aagcagatcc aacaacgact gggggaaacg caatgattat tgaacgttgt
     2461 cgtgaactgg cgttgcgagc gcccgccaga gtggtttttc cggatgcgtt agaccaacgt
     2521 gtgctgaaag ccgcgcaata tttacatcaa caaggtctgg caacgcccat tctggtcgcc
     2581 aatccgtttg aacttcgtca gtttgcgctc agtcacggcg tggcgatgga cgggctacag
     2641 gtgattgatc cgcatggcaa cctcgcaatg cgggaagaat ttgctcatcg ctggctggcc
     2701 cgcgcgggcg aaaaaacgcc gccggatgcg ctggaaaaac ttaccgaccc tctgatgttc
     2761 gccgcagcaa tggtcagcgc cggtaaagcg gatgtctgta tcgcgggcaa cctctcttcc
     2821 acggcaaatg tgctgcgtgc cggattacgc attatcggct tgcagccagg ctgtaaaacg
     2881 ctctcttcca ttttcctgat gttgccacag tacagcggcc cgccgttggg ctttgccgat
     2941 tgcagcgtgg tgccacagcc gacggcggcg cagctggcgg atatcgcgct tgccagtgcc
     3001 gaaacctggc gcgccatcac cggagaagag ccgcgcgtgg cgatgctgtc gttttccagc
     3061 aatggtagcg cccgtcaccc ctgtgttgct aacgtccagc aggcgacaga aatcgtccgt
     3121 gagcgcgcac caaagctggt ggtggatggc gagttacagt ttgacgccgc cttcgtgccg
     3181 gaagtggcgg cgcaaaaagc gcctgccagc ccgctacagg gcaaggccaa tgtgatggtt
     3241 tttccgtcgc tggaagccgg aaatattggt tacaaaatcg cacaacgact cggcggatat
     3301 cgtgccgtcg ggccactgat acaaggactt gccgcgccga tgcacgatct ctctcgtggt
     3361 tgtagcgtgc aggaaattat cgagctggcg ctggtggcag ctgtgccgcg tcagacagaa
     3421 gtgaaccgcg aaagcagttt acaaacactg gttgaatgaa cggtcccgtt ctggaccctg
     3481 ataaagagga aagcacaatg gaagcattag gaatgatcga aacccggggc ctggttgcac
     3541 tgattgaggc ttctgacgcg atggtgaaag ctgcgcgcgt caagctggtt ggtgttaagc
     3601 agattggcgg cggcctgtgt actgccatgg ttcgtggtga tgttgcagcc tgtaaagccg
     3661 cgaccgatgc aggtgccgca gcggcacagc gtatcggcga gctggtatct gtacacgtta
     3721 tcccgcgtcc gcacggcgac ctggaagaag tcttcccgat cggcctgaaa ggcgatagca
     3781 gcaacctgta atcaccctgt gtgacgtcga ttcgggcgtc acgctctctc ctttttcatt
     3841 ttaccttctg cggagggtag ggggagcttt tacctgaaat acgccacgga ggcgggtatg
     3901 aaactggcag tcgtcactgg acaaattgtt tgtaccgtac gccatcacgg actggcgcat
     3961 gacaaattgc tgatggtgga aatgattgat ccacaaggta atcctgacgg gcaatgcgcc
     4021 gtcgccatcg acaatattgg cgcgggaacc ggggagtggg tgttactggt gagtggcagt
     4081 tccgcccgcc aggcgcataa aagcgaaacg tcaccggtcg atctgtgcgt gattggcatt
     4141 gtcgatgagg tggtgtctgg cggtcaggta attttccaca aataaggcag aacatcatga
     4201 atcaacagga tattgaacag gtggtgaaag cggtactgct gaaaatgcaa agcagtgaca
     4261 cgccgtccgc cgccgttcat gagatgggcg ttttcgcgtc cctggatgac gccgttgcgg
     4321 cagccaaagt cgcccagcaa gggttaaaaa gcgtggcaat gcgccagtta gccattgctg
     4381 ccattcgtga ggcaggcgaa aaacacgcca gagatttagc ggaacttgcc gtcagtgaaa
     4441 ccggcatggg ccgcgttgaa gataaatttg ccaaaaacgt cgctcaggcg cgcggcacgc
     4501 caggcgttga gtgtctctct ccgcaggtgc tgaccggcga caacggcctg actttaattg
     4561 aaaacgcgcc ctggggcgtg gtggcttcgg taactccgtc cactaacccg gcggcaaccg
     4621 taattaataa cgccatcagc ctgattgccg cgggcaacag cgtcattttt gccccgcatc
     4681 cggcggcgaa aaaagtctcc cagcgggcga ttacgctgct caaccaggcg attgttgccg
     4741 caggtgggcc ggaaaactta ctggttactg tggcaaatcc ggatatcgaa accgcgcaac
     4801 gcttgttcaa gtttccgggt atcggcctgc tggtggtaac cggcggcgaa gcggtagtag
     4861 aagcggcgcg taaacacacc aataaacgtc tgattgccgc aggcgctggc aacccgccgg
     4921 tagtggtgga tgaaaccgct gacctcgccc gtgctgctca gtccatcgtc aaaggcgcgt
     4981 ctttcgataa caacatcatt tgtgccgacg aaaaggtact gattgttgtt gatagcgtag
     5041 ccgatgaact gatgcgtctg atggaaggcc agcacgcggt gaaactgacc gcagaacagg
     5101 cgcagcagct gcaaccggtg ttgctgaaaa atatcgacga gcgcggaaaa ggcaccgtca
     5161 gccgtgactg ggttggtcgc gatgcggcga aaatcgcggc ggcaatcggc ctgaacgtgc
     5221 cgcaagaaac gcgtttgctg tttgtggaaa ccactgcaga acatccgttt gccgtgaccg
     5281 aactgatgat gccggtgctg cccgtcgtgc gcgtcgccaa cgtggcggat gccattgcgc
     5341 tggcggtgaa actggaaggc ggttgccacc acacggcggc aatgcactcg cgcaacatcg
     5401 aaaacatgaa ccagatggcg aatgctattg ataccagcat tttcgttaag aacggaccgt
     5461 gcattgccgg gctggggctg ggcggggaag gctggaccac catgaccatc accacgccaa
     5521 ccggtgaagg ggtaaccagc gcgcgtacgt ttgtccgtct gcgtcgctgt gtattagtcg
     5581 atgcgtttcg cattgtttaa ggagtaacag atggcgcacg acgaacaatg gctcacccca
     5641 cgtctgcaaa cggcggcaac gctgtgtaac cagacgcccg ccgcgacgga atctccgctg
     5701 tggctggggg tggatctggg cacctgcgat gtggtgtcga tggttgtcga ccgcgacggt
     5761 cagccggtgg cggtatgcct cgactgggcc gatgtggtcc gcgacggcat cgtctgggat
     5821 ttcttcggcg ctgtcaccat tgttcgtcgc catctcgaca cgctcgagca gcaattcggt
     5881 cgccgtttta gccatgcggc gacttcattt cctccaggta cggacccgcg tatttccatc
     5941 aacgtgctgg agtctgccgg gctggaagtg agccatgtgc tggatgagcc aaccgcagtc
     6001 gcggatctac tgcaactgga taacgcaggc gtagtggata tcggcggtgg cactaccggc
     6061 attgccatcg tgaaaaaggg caaagtgacg tactcggcgg atgaagccac cggcggacat
     6121 cacatctctc tgactctcgc cggaaatcgc cgtatttcgc tggaagaggc ggagcagtac
     6181 aagcgcggtc acggtgacga gatttggcct gcggtgaaac cggtgtacga aaaaatggcg
     6241 gacattgtcg cccggcatat tgaagggcag ggcattactg atttatggct ggcgggcggc
     6301 tcctgtatgc aaccgggcgt ggcagaactg tttcgcaaac aattcccggc gttacaggtg
     6361 catttaccgc agcacagttt gtttatgacc ccgctggcga tcgccagtag tgggcgagag
     6421 aaagcggagg ggctatatgc aaaatgaatt gcagaccgcg ctctttcagg cgttcgatac
     6481 cctgaatctg caacgggtaa aaacatttag cgttccaccg gtgacgcttt gcggtctggg
     6541 cgcggtgagc agttgcgggc agcaagcgca aacgcgtggg ctgaaacatc tgttcgtgat
     6601 ggcagacagc tttttgcatc aggcagggat gaccgccggg ctgacgcgca gcctggccgt
     6661 taaaggcatc gccatgacgc tctggctatg tccggtgggc gaaccgtgca ttaccgacgt
     6721 gtgtgcagcc gtggcgcagt tgcgtgagtc aggttgtgac ggggtgatcg catttggcgg
     6781 cggctcggtg ctggatgcgg cgaaagccgt ggcgttgctg gtgacgaacc cggatagcac
     6841 gctggcagag atgtcagaaa ccagcgttct gcaaccgcgc ttgccgctga ttgccattcc
     6901 aactaccgcc ggaaccggct ctgaaaccac caatgtaacg gtgattatcg acgcggtgag
     6961 cgggcgcaag caggtgttag cccatgcctc gctgatgccg gatgtggcga tcctcgacgc
     7021 cgcattgacc gaaggtgtgc cgtcgcatgt cacggcgatg accggcattg atgcgttaac
     7081 ccatgccatt gaagcataca gcgccctgaa cgctacaccg tttaccgaca gtctggcgat
     7141 tggtgccatt gcgatgattg gcaaatcgct gccgaaagcg gtgggctacg gtcacgacct
     7201 tgccgcgcgc gagagcatgt tgctggcttc atgtatggcg ggaatggcgt tttccagtgc
     7261 gggtcttggg ttgtgccacg cgatggcgca tcagccgggc gcggcgctgc atattccaca
     7321 cggtctggcg aacgccatgt tgctgccaac ggtgatggaa tttaaccgga tggtttgtcg
     7381 cgaacggttt agtcagattg gtcgggcact gcgaactaaa aaatccgacg atcgtgacgc
     7441 tattaacgcg gtaagtgagc tgattgcgga agttgggatt ggtaaacgac tgggcgatgt
     7501 tggcgcgaca tctgcgcatt acggtgcatg ggcgcaggcc gcgcaggaag atatttgtct
     7561 gcgcagcaac ccgcgtaccg ccagcctgga acaaattgtc ggactgtacg cggcggcgca
     7621 ataaatgccg gatgcgacgc ttgccgcgtc ttatccggcc tacgggattg cacatgtagg
     7681 gcggataagg cgtttacgcc gcatccgcca ataaataaca actaacaggg agtaaaggcg
     7741 atgggaatta acgaaatcat catgtacatc atgatgttct ttatgctgat agctgccgta
     7801 gacaggatcc tgtcgcagtt cggcggttct gctcgtttcc tcggtaagtt cggtaaaagt
     7861 atcgaaggat caggcggtca gttcgaagaa ggctttatgg caatgggcgc tctgggtctg
     7921 gcgatggtcg gtatgaccgc gctggcaccg gtactggctc acgtactcgg gccggtaatt
     7981 atcccggttt acgaaatgct cggcgcaaac ccgtcaatgt tcgccggaac actgctggcg
     8041 tgcgatatgg gcggcttctt cctcgccaaa gagctggcgg gcggcgacgt agcagcgtgg
     8101 ctatactctg ggttaattct cgggtcgatg atggggccaa cgattgtgtt ttccattccg
     8161 gtggcgctcg gcattatcga accttctgat cgtcgttatc tggcgctcgg cgtgctggcg
     8221 ggcattgtga ccattccgat tggctgtatt gctggtggtc tggttgctat gtactccggt
     8281 gtgcagatca acggtcagcc ggtggaattc actttcgccc tgatcctgat gaacatgatc
     8341 ccggtgatca ttgtcgcgat tctggtggcg ctggggctga aattcatccc ggaaaaaatg
     8401 atcaacggct tccagatctt tgccaaattc ctcgttgcat tgatcaccct cggtcttgcc
     8461 gctgcggtag tgaaattcct ccttggctgg gaactgatcc ccggactgga tcctatcttt
     8521 atggcccctg gcgataaacc cggtgaagtg atgcgcgcca ttgaagttat cggttctatc
     8581 tcctgcgtgc tgttaggggc gtatccaatg gtgctgctgc tgactcgctg gtttgaaaaa
     8641 ccgctgatga gcgtcggtaa agtactgaat atgaacaaca tcgcggcagc cggcatggtg
     8701 gcaacgcttg ccaacaacat cccgatgttc ggcatgatga agcagatgga tacccgcggc
     8761 aaagtcatca actgcgcctt cgccgtttcc gctgctttcg ccctgggcga ccacttaggc
     8821 ttcgccgctg ccaacatgaa cgccatgatc ttcccgatga ttgtcggcaa gttgatcggc
     8881 ggcgtaacgg cgattggcgt ggcgatgatg ctggtgccaa aagaagacgc gaccgcgact
     8941 aaaaccgaag cggaggcaca atcgtgaaca ctcgccagct attgagcgtc ggtatcgata
     9001 tcggcaccac caccacccag gtgattttct cccacctgga actggttaac cgtgcggcgg
     9061 tgtcgcaggt gccgcgctac gaattcatta aacgcgaaat tagctggcaa agcccggtat
     9121 tctttacccc tgtcgataaa cagggtggtt taaaagaagc ggaactgaaa tcgctgattt
     9181 tagagcaata tcaggctgcg ggcattgcgc cggaaagcgt tgattctggt gccatcatca
     9241 tcaccggtga aagcgcgaaa acccgcaatg ctcgcccggc ggtgatggcg ctctctcagt
     9301 cgctgggcga ttttgtcgtt gccagcgccg ggccgcacct cgaatccgtg atcgccggtc
     9361 acggagccgg ggcacaaacc ctttctgaac aacggctgtg tcgggtactg aatatcgaca
     9421 tcggcggtgg caccgcgaac tacgccctgt tcgatgccgg aaaaatcagc ggcactgcct
     9481 gtctcaacgt cggtggtcgc ctgttggaaa ccgacagcca ggggcgcgtg gtttacgctc
     9541 ataaaccggg gcagatgatt gtggatgagt gcttcggtgc aggcactgac gcccgttcgc
     9601 tgaccggcgc gcagctggtg caggttaccc ggcggatggc ggcgctgatt gtcgaagtga
     9661 ttgacggaac gctttcgccg ctcgcgcagg cattgatgca aaccggtttg ctgcccgcag
     9721 gtgttacgcc cgaaatcatt acgctttctg gaggcgtggg cgaatgttat cgccaccagc
     9781 ccgccgaccc gttctgtttt gccgatattg gcccacttct ggcaacggcg ctgcatgacc
     9841 atccgcgcct gcgtgagatg aatgtgcagt ttccggcgca aaccgtacgc gccacggtga
     9901 ttggcgcggg tgcacatacc ctttcgctct ctggcagcac aatctggctg gagggcgtac
     9961 aactgccgct gcgcaatttg ccggtggcga tcccgattga tgaaacggat ctggtgagtg
    10021 cctggcaaca ggcgctgctt cagctggatc ttgatcccaa aactgacgcg tacgtgctgg
    10081 cgcttcccgc ctcgctgcct gtgcgttacg ccgcggtact gacggtcatc aacgcgctgg
    10141 tcgatttcgt cgcgcgtttt ccgaatccgc atcccctgct ggtggtggcc gggcaggact
    10201 ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca acaactcccg ttggcagtca
    10261 ttgacgaagt gattgtccgc gcgggggact atatcgacat tggtacgcct ctttttggcg
    10321 gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc ttcctgaggg aacgacttat
    10381 gaaactaaag accacattgt tcagcaatgt atatcagttt aaggatgtaa aagaggtgct
    10441 ggctaaagcc aacgaactgc gttcggggga tgtgctggca ggcgttgcag cggcaagttc
    10501 acaggagcgc gtggcggcaa agcaggtgtt gtcggaaatg accgtagcgg acatccgcaa
    10561 taatccggtg attgcctatg aagatgactg cgtgacgcgg ctgattcagg acgatgttaa
    10621 cgaaacggcc tacaaccaga ttaaaaactg gagcatcagc gaactgcgtg agtatgtgct
    10681 gagcgatgaa accagcgtgg acgacattgc ctttacccgc aaagggctga cctcggaagt
    10741 ggtcgcggcg gtagcgaaga tttgctccaa cgcggacctg atctacggcg cgaagaaaat
    10801 gccggtaatc aaaaaggcca ataccaccat tggtattccg ggcaccttca gcgcccgttt
    10861 gcagccaaat gacacccgtg acgacgtgca aagtatcgcc gcgcaaatct acgaagggct
    10921 ttccttcggg gtgggcgatg cggtgatcgg cgttaacccg gtgactgacg acgtggaaaa
    10981 cttaagccgc gtgttggaca ccatttacgg cgtgatcgac aaattcaaca tcccaactca
    11041 gggctgcgta ctggcgcacg tcaccaccca gatcgaagcg attcgtcgcg gcgcgcctgg
    11101 cggactgatt ttccagagta tctgtggcag cgaaaaaggg ctgaaagagt ttggcgtgga
    11161 actggcgatg ctcgacgaag cgcgcgcagt gggtgcagag ttcaaccgta tcgccgggga
    11221 aaactgcctc tacttcgaaa ccggacaagg ctctgcgcta tccgctggcg ctaacttcgg
    11281 cgctgaccag gtgacgatgg aagcacgtaa ctatgggctg gcgcgtcatt acgatccgtt
    11341 tatcgtcaac accgtggtcg gctttattgg gccggagtat ctctacaacg accgccagat
    11401 tatccgtgct ggcttagaag atcactttat gggcaagctg agcggcatct ctatgggctg
    11461 tgactgctgt tacaccaacc acgctgacgc tgaccagaac ctcaacgaaa acctgatgat
    11521 cctgctcgcc accgcaggct gcaactacat catggggatg ccgctgggtg atgacatcat
    11581 gctcaactac cagaccaccg cattccacga taccgccact gtgcgtcagt tactcaacct
    11641 gcgtccgtca ccggagtttg aacgctggct ggaaagcatg ggcattatgg caaacggtcg
    11701 cctgaccaaa cgggcgggcg atccgtcact gttcttctga tgacgcggag ataacacatc
    11761 atggatcaaa aacagattga agaaattgta cgcagcgtga tggcgtcaat gggacaaacg
    11821 gcccccgcgc cgtcagaagc aaagtgcgcc accaccaact gtgcggcacc ggtgacctcg
    11881 gaaagctgtg cgctggattt aggttccgct gaagcaaaag cgtggattgg tgttgaaaac
    11941 ccgcatcgcg cagacgtatt aacagaactg cgccgcagca ccgtggcccg cgtttgtacc
    12001 ggtcgtgccg gtccgcgtcc acgtacccag gcgctgctgc gtttcctggc cgatcactct
    12061 cgttcgaaag ataccgtact gaaagaagtg ccggaagagt gggtgaaagc gcagggcttg
    12121 ctggaagtac gctctgaaat tagcgacaaa aatctctacc tgactcgccc ggatatgggc
    12181 cgccgcctgt gtgcagaagc tgttgaagcg ctgaaagcac agtgtgttgc taatccggac
    12241 gtacaggtcg ttatttctga tggcctgtca actgatgcca tcaccgtgaa ctacgaagag
    12301 atcctgccgc cgctgatggc gggcctgaaa caggccgggc tgaaagtcgg cacgccattc
    12361 tttgttcgtt atggtcgcgt gaagattgaa gatcagatcg gtgagatcct cggcgcgaaa
    12421 gtggtgatcc tgctggtggg ggaacgtccg gggttagggc agtcagaaag cctctcctgc
    12481 tacgccgttt actcgccgcg tatggcgacc accgttgagg ccgatcgcac ctgtatctct
    12541 aacattcacc agggcggcac gccgccggtt gaagccgcag ccgtcattgt ggatttggcg
    12601 aaacgtatgc tggagcagaa agcatccggc atcaacatga cccgataagg aggcatcatg
    12661 ccagctttag atttgattcg accgtcggta accgccatgc gggtgattgc ctccgttaac
    12721 gctgaatttg cgcgtgaact aaaattgccg ccgcatattc gtagcctcgg gctgatttct
    12781 gctgattccg atgacgtcac ctacattgcc gctgatgaag cgaccaagca ggcgatggtg
    12841 gaagtggtat atggtcgctc gctgtatgcg ggcgctgcac acggcccgtc accgactgcc
    12901 ggtgaagtgc tgattatgct tggtgggcca aacccggcgg aagtgcgtgc cggtctggat
    12961 gcgatggttg cacatattga aaatggtgca gcattccagt gggccaatga cgcagaaaac
    13021 accgccttcc tggcgcatgt ggtttcgcgt accggttctt atctctcatc aaccgccggg
    13081 atcacgcttg gcgatccgat ggcgtatctg gtggcaccgc cgctggaagc gacctatggc
    13141 attgatgcag cgttgaaatc tgccgacgta cagctggtga cctatgtccc gccaccgtct
    13201 gaaaccaact actcggcagc atttttaacc ggtagccagg ccgcgtgtaa agcagcctgt
    13261 aacgccttta ccgatgcagt gctggaaatc gcgcgtaatc caatccagcg tgcgtaacgg
    13321 aggttgccga tgatcaatgc actgggattg ctggaagtgg acggcatggt cgccgcgata
    13381 gatgcagcgg atgccatgct caaagcagct aacgttcgtc tgctcagtca cgaagtgctt
    13441 gaccctggtc gcttaacgct ggtggtggaa ggcgatctgg cggcgtgtcg tgcggcgctg
    13501 gacgcaggtt gtgctgccgc gatgcgtacc gggcgtgtca tcagccgcaa ggagatcggt
    13561 cggccagacg atgacaccca gtggctggtt actggcttta accgccagcc gaagcaaccc
    13621 gtaagggaac ccgacgcgcc agttatcgtc gcggaatctg ctgacgagtt gttggcgctg
    13681 ttaacatcag tacgtcaggg aatgacggca ggagaagtgg ctgcccactt tggctggccg
    13741 ctggaaaaag ccagaaatgc gctcgaacag ctcttttctg ccgggacgtt acgtaaacgc
    13801 agtagtcgtt atcgtctcaa gccccattaa cctgtcggag gtgccgggtg tcctacaaca
    13861 cccggcatta acatcatgaa aaagacccgt acagccaatt tgcaccatct ttatcatgaa
    13921 cccttacccg aaaacctgaa gctcacgccg aaggtcgaag tggataatgt tcatcaacga
    13981 cagacaacgg atgtctatga acatgcttta acgattaccg cctggcagca gatttacgat
    14041 cagctgcatc cgggcaagtt tcatggtgaa tttacggaaa ttctactcga tgatattcag
    14101 gtttttcgtg aatacaccgg tctggcgctg cgtcagtcgt gcctggtctg gccgaactcg
    14161 ttctggtttg gcattccggc gacgcgcggt gagcagggat ttatcggttc gcaatgtctg
    14221 ggaagcgcgg aaatcgccac ccgccctggt ggcactgaat ttgaactgag cacgccggat
    14281 gattacacga tcctgggcgt ggtgctttct gaagatgtca tcacccgaca ggctaacttt
    14341 ttgcataacc cggatcgggt attacatatg ctgcgtagcc agtcggcgct ggaagtgaaa
    14401 gagcagcata aagccgcgct gtggggcttt gtccaacagg cgctggcgac gttttgcgag
    14461 aacccggaaa atctccatca gcctgcagtg cgaaaagtgc tgggggataa tttgctaatg
    14521 gcgatggggg cgatgctgga agacgcgcaa ccaatggtga cggcggaaag catcagtcat
    14581 cagagttacc gtagattact ttcccgcgcc cgtgaatatg tgctggaaaa tatgtctgag
    14641 ccggtgacgg tgctggactt gtgtaatcaa ctgcatgtca gtcgccgcac gctacaaaac
    14701 gcgtttcacg ctattttagg cattggccca aacgcgtggc tgaaacgcat tcgcctgaac
    14761 gccgtacgcc gcgaactgat aagcccgtgg tcgcaaagca caacggtaaa agacgccgcc
    14821 atgcagtggg gattctggca tctggggcaa tttgccacgg attaccagca gctgtttgcc
    14881 gagaagccgt cgttgacgtt gcatcagcgg atgcgggaat gggggtgagg gagttacacc
    14941 caatccctga ccttaataaa ctcacttaac gccgcttctg ggctgccatc ttttggctga
    15001 taatcatact cccagcgtac cagtggcggc attgacatca ggatcgactc ggtgcgcccg
    15061 ccagtttgca ggccaaacag agtgccgcga tcccagacca gattgaactc gacataacga
    15121 ccgcgacggt agagctgaaa attgcgctcg cgctcgccgt aggccatcgc tttacgtcgc
    15181 tctacaattg gtaaataagc gtcggtgtag cctttgccta ccgcctgcat aaaggcaaaa
    15241 cagtggtcga aatctggcgt gttcagatca tcaaagaaca gcccgccaat accgcgctgt
    15301 tcgttgcgat gtttgaggta gaagtaatcg tcgcaccact ttttgtaacg gggataaacg
    15361 tcttcaccaa atggcaggca caggtcacgg gcggtgcggt gccagtgaat ggcgtcttct
    15421 tcaaaaccat agaaaggggt taaatcgaag ccgccgccaa accaccagac gggctctgca
    15481 cccggttttt cggcaataaa aaaacgcaca ttcgcgtggc tggtgggaac atacgggtta
    15541 tgcggatgca ctaccagtga aacgcccatc gcctcgaaac tgcgcccggc aagttccggg
    15601 cgatgagcgg tggcggaagc tggcatcgcc tcgccgtgga cgtgagaaaa gttgacgcct
    15661 gcctgttcga aaacaccacc attacgcaac acccgactac gcccgccgcc gccagcttcg
    15721 cgctgccaac tatcttcgac aaattctgcg ccatcgacgg cggtcagctg ctgacaaatc
    15781 gtatcctgaa ggttgagcag aaactgttta acctggtgtg cgtcgggttt cataacttat
    15841 cgctttttcg aatgtgcttt ctggttgtcg aaccagtgga aataactgat cacgccttca
    15901 gcaatcgctg tggcgatttt ctgacgaaac gccgccgtgc ctaacagccg ctcttcttcc
    15961 gggttggtga taaacgaggt ttccaccagc accgaaggaa ccgacggcga tttcaacacc
    16021 acaaacgcgg cttgttcggt gttgcggctg tgcagtttat gcaccggctt aatctttttc
    16081 agaatatgcg agccgagcgt caggctgttt ttaatagtat cggtttgcac cagatcaaac
    16141 agcacttgct gcaataggtg atccttgtca gtcgcctttt taccggcaac ttcatcggcg
    16201 cggttttcgc gttcagacag gtatttcgcc attgcgctac ttgccccacg gttagagagg
    16261 gcaaataccg aagcaccggc agctttcggg ttggtaaagc catcggcatg aattgacata
    16321 aacagatctg cgccatgttt atgggcgatt tcaacgcgat cgtaaagtgg gataaacgta
    16381 tcgccagaac gcgttaaacg cgcatcaatc ccatgattac gcaaaatgga acggacgttt
    16441 ttagcaatcg ccagcaccac atgtttttct ttcgaaccgt tgcgtccgat cgctccggta
    16501 tcaattccgc cgtggcctgg atcgagaaca acgacacgtt tgccgccaga ttttttggct
    16561 ttcggcttgc tgtgtccgtt gctggttttt aaaggttcgt ctttggcgat ggcttgcgac
    16621 attcctgaca acgtcagggc agccaaaccg gctttcagca cctggcggcg cgaagtgagt
    16681 gtttttagtg gtttaaaagt gctcatacgg cctgagttgt aataataaag ttccagatgt
    16741 tatatcgtat cgcgtaatcc gttacgacgg tttgatgttg agaattgttt tacttttcat
    16801 ttcaatagtt gacaactccc cattatgcca ctttttcaac cgattttcgt cagatattgg
    16861 ctaattcgac cgttcacgcc ataatcagcc ttttaacccg caaaaaggta acgaatacca
    16921 tggagatacg cgtatttcgc caggaagatt tcgaagaggt catcaccctt tgggagcgtt
    16981 gcgacttgct gcgtccgtgg aacgatccgg aaatggacat cgagcgtaag atgaaccatg
    17041 acgtcagttt gtttttggtc gctgaggtaa acggtgaagt ggtcggaacg gtgatgggcg
    17101 gttatgacgg gcatcgcggg tctgcttatt atcttggcgt gcatccagag tttcgtgggc
    17161 gtgggattgc caatgcgttg cttaatcggc tggagaaaaa gctgattgct cgtggctgcc
    17221 cgaaaattca gatcaatgtg ccggaagata acgacatggt gctcggaatg tatgaacgcc
    17281 tgggatatga acacgccgac gtgctgagtc tgggtaagcg tttgattgaa gatgaagagt
    17341 actgagttcc atcctgtcca ttatgatgcg cacggacgcc tgcgtttacc cttgctcttc
    17401 tggcttgtgc tattgcttca ggcgcgaacc tgggtgctgt ttgtcattgc cggtgcgtcg
    17461 cgtgagcagg gtactgcgct gctaaatctg ttttatcccg atcacgataa tttctggctg
    17521 gggctgattc ctggcattcc ggcggtgctg gcgtttttgt tgagcggtcg gcgggctaca
    17581 tttcctcgca cttggcatgt gctctatttt ctgttgctgt tggcgcaggt ggttttactt
    17641 tgctggcaac cgtggctgtg gctgaacggt gaatccgtta gcggtatcgg tctggcgctg
    17701 gtagtggcgg atattgtggc gttaatctgg ctgctgacca atcgacgttt acgcgcttgt
    17761 tttaatgaag taaaagaata acggcacttt ttggtgaatt tgcactccaa gcaacgttat
    17821 tgaataacca aaggaagtga catgaaatcg ctgcgtttaa tgttatgcgc tatgccattg
    17881 atgctgaccg gctgttccac gatgtcgtca gttaactggt ctgccgctaa cccgtggaac
    17941 tggtttgggt cgtccaccaa agtgagcgag cagggcgttg gtgaattaac ggcgtccaca
    18001 ccactgcaag aacaagccat tgccgatgcg cttgatggcg attatcgcct gcgcagcgga
    18061 atgaaaaccg cgaacggcaa cgtggtgcgc ttttttgaag tgatgaaggg cgacaacgtg
    18121 gcgatggtga ttaacggcga tcaaggcacg atcagtcgca ttgacgtgct ggatagcgat
    18181 attcctgctg acaccggtgt taaaatcggt acaccgttta gcgaccttta cagcaaagca
    18241 tttggcaatt gccaaaaagc tgatggtgat gataatcgtg ccgtcgaatg taaagccgaa
    18301 ggcagtcaac atattagcta ccagttcagc ggggaatgga gtggtcctga agggttaatg
    18361 ccttcggacg ataccctgaa aaactggaaa gtcagtaaaa ttatctggcg gcgttaattt
    18421 gcgtctgaac aaaccgccgc cgcgaaaaaa cacgtaaaat agcgctcaac aatgccacgg
    18481 attgcgtggc attattattt tcaggaggaa caatgtctca ggttcagagt ggcattttgc
    18541 cagaacattg ccgcgcggcg atttggatcg aagccaacgt gaaaggggaa gttgacgccc
    18601 tgcgtgcggc cagtaaaaca tttgccgaca aactggcaac ttttgaagcg aaattcccgg
    18661 acgcgcatct tggtgcggtg gttgcctttg gtaacaacac ctggcgcgct ctgagcggcg
    18721 gcgttggggc agaagagctg aaagattttc cgggctacgg taaaggcctt gcgccgacca
    18781 cccagttcga tgtgttgatc cacattcttt ctctgcgtca cgacgtaaac ttctctgtcg
    18841 cccaggcggc gatggaagcc tttggtgact gcattgaagt gaaagaagag atccacggct
    18901 tccgttgggt tgaagagcgt gacctgagcg gctttgttga cggtacggaa aacccggcgg
    18961 gtgaagagac gcgtcgcgaa gtggcggtta tcaaagacgg cgtggatgcg ggcggcagct
    19021 atgtgtttgt ccagcgttgg gaacacaacc tgaagcagct caaccggatg agcgttcacg
    19081 atcaggagat gatgatcggg cgcaccaaag aggccaacga agagattgac ggcgacgaac
    19141 gtccggaaac ctctcacctg acccgcgttg atctgaaaga agatggcaaa ggactgaaga
    19201 ttgttcgcca gagcctgccg tacggcaccg ccagtggcac tcacggtctg tacttctgcg
    19261 cctactgcgc gcgtctgcat aacattgagc agcaactgct gagcatgttt ggcgataccg
    19321 atggcaagcg tgatgcgatg ttacgtttca ccaaaccggt cactggcggc tattacttcg
    19381 cgccgtcgct ggacaagttg atggcgctgt aagcgtttct gattcgatga tgaaaggcca
    19441 ggcagtaaca ttactgactg gcctttttat tactgcttta acgccgcata tacctgatca
    19501 accacccaac cataagttgc aattggcaac tgaccgcttt cgaacatatt gggattcttt
    19561 tgcggatcgg caaccggtga atgtggcttg ttacttaaca tcacaatcgc catatgattc
    19621 accggatcga taacggtcac cgttccggtc cagccagtgt gaccgtaggt ttgcgggctt
    19681 gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat ttacgcgcca gccgaggcca
    19741 aaagtggcat cttccttaga gctggtggtg aacatcttca ccgtttccgc actgaacagc
    19801 tgcacatcgc catagccccc gccgttcagc atcgtttgca ttaacaccgc aatatcgccg
    19861 gtattggaaa acaaacctgc gtgcccggaa acgccgccca tcgaataaaa ggctttttca
    19921 tcgtgcacct gaccccagag agtggaggtg cggatattcg ggaaatggat cacgccatcg
    19981 cgggtattgc cgttcaattc cgtggcggca atttgttgcg gtttaaagcc tttcagcagc
    20041 gggttaaata ccgtatgtgt caggccgagc gggcgataaa tcgactcttc aacatagcgg
    20101 tcaagcggct gaccggtgac cgactcgacg ataaatccaa gcagcatata atcgacatca
    20161 ctgtaaatat gcttgctgcc gggctgatac tctagcggcg tgcgcttgat catctccagc
    20221 gtctggcctt tatcctggga atataacgcg cccgcgaccg ctttattcgg gtattgcgga
    20281 tctgccggga aaccgccgct gtgatgcagc aggtcagaaa tccgcagggt gtttttgcct
    20341 ttaatggtgt catttggact atcggcaaac cccggaatat atttcgcaat ccgatcgtca
    20401 ggatgcagtt tgccttcgct catcagcttt tgcagggcga agttcgtggc gtacattttg
    20461 gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt tgacaggctg ttccatcaac
    20521 acgctgccat cgtacttttt cgccgctccc caggctttgc gatacacaat ctgattatct
    20581 ttaatgatca gcaggtttac gccgggataa ccggcgtcca cttgctggct aatccagcga
    20641 tccatctggt taagccgttc gacgttgaac cctgctttct ctggcgaact ttctgtcaga
    20701 acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag atagagcatt
    20761 gtccgtttca tgatatcagt ccagattgac gttacggcag ccaaagagcg tggtgaaaat
    20821 aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccattg ccggaaggat
    20881 gccttgtgcg gaagtcatca gcggcagcgc caccagacca gacggcccaa aggcgctgtt
    20941 taagcccatc ggcagacccc accaggctat caggccgata aacaaaccgc ccgctgcgcc
    21001 gcctaaacag gcggtaataa acggtttcat gcggggcagg gtgacgccgt aaatcaacgg
    21061 ttcaccaacg cccagcagac cgggaataat cgccccgcgt acctgactgc gtaatgcact
    21121 gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg cgcctgccat
    21181 tgaaaggatg ggaaataagc tgttgaatcc ctggctgtcc attaacgcga ggtaaacagg
    21241 aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg ctaaaaccgc
    21301 acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt cgaataacca
    21361 gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca gggtgattaa
    21421 cgaggtcagc agcatgtcga gatcgtccgg cataaagcgg cgcaccatgc cttcaatgcg
    21481 ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat cgatgggcag
    21541 accaaagaaa tcgtgaaaac cggcgtagta accggtggtt gcagcggggt tataaccgag
    21601 caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg cctgggcggc
    21661 gttatagccc accagaatca ccaggaaagt gaacaaacct ttgctgaaca ccttcataaa
    21721 attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca ccgtggcaat
    21781 taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca gcggcgtgaa
    21841 gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg cttttaactg
    21901 acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc ccagcagctc
    21961 gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct gcacctggtc
    22021 gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat caaccagtga
    22081 actgtcatgt acacccagac gcaggcgcgt catacagtta ccacaactgg cgatatttcc
    22141 cggtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct ctttggccat
    22201 gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca ggcgtttttt
    22261 agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac agttgcgctc
    22321 gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt ttttaacgat
    22381 attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca tcaggttgcc
    22441 gaacactttg ccggatttaa tcatcagccc ggtggagagc atattgagca ccagtttctg
    22501 cgctgtacct gctttcatcc gcgaagaacc ggtaaccact tcggcaccta caatcggtgt
    22561 aatggcaaac tctgcggtgg ttgaaacggc gctccccgga ttacaggaaa ttcccactgt
    22621 gcggcagccg agctggcgtg cgtattccag tccggcaatt acatacggcg tgcgaccact
    22681 ggccgcaatg ccaaccacca catcctgcgc ggttaagccg atgtttttca gatcgttaac
    22741 accaccttcc cggctatctt cagcgccttc caccgcatgc tgaatggcat attcgcctcc
    22801 agcaatcaaa ccaaccacca aacccggttt cacgccgtag gtgggcggac attcgctggc
    22861 atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac gaccgccgcc
    22921 gcttacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagta cgcgctcaac
    22981 ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca gcgttgatac
    23041 gcggtcaatt tcagccgagg cggcgttcga gccttcggta atcatctttt caagttgcat
    23101 cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc atatatcccg
    23161 aattgacaga cttaaaggaa tcattgagtc tacactcgca gagtgtgaag agcgtcataa
    23221 gaacttaagc ggaatttttg ctgccagccc aggagagtgg aagatgttgt acctgacgaa
    23281 aatacgcaat gcggaaagtg aatttaccga gaacgagcaa aaaattgctg actttctgcg
    23341 ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag atggcgaaac agctcggcat
    23401 cagccagtcg agtattgtga agtttgccca gaagctcggc gcgcaaggat ttactgaatt
    23461 gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg ccacggcgct
    23521 gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc gcaaactgaa
    23581 tcgcgaaaaa gagctggcgc tggagcaaac ctgcgcgttg ttcgactacg cgcggctgca
    23641 aaaaatcatt gatgtcatca gtaaagcgca atttattcag ataacgggcc tgggcggatc
    23701 ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc gcgtggcctg
    23761 cgaagccgac acgcacgtac aagccaccgt ttcccaggcg ttgaaaaaag gcgatgtaca
    23821 aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag aagcggcacg
    23881 aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc tgcggcggct
    23941 ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct cgtcgatgtc
    24001 tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg tgcaactcaa
    24061 tgatgtcgag tcattgaaaa tgattcagcg cagcagtgag ctaacacaac gcctgaaatg
    24121 atgcataaag cagcgactgg attgagattt tcctgaatta gtgagctgat ccgcagcaat
    24181 attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat taacaaggag
    24241 agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc attgcaggga
    24301 attggcgaag gaattgccag aacttttgcg cgtcatggcg caaacctaat cttgctggat
    24361 atctcccctg agatcgaaaa gctggcggac gaactgtgtg gtcgtggtca tcgctgtacg
    24421 gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa acgcgcgaag
    24481 gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg tctgggcagt
    24541 ttcctcgata tgagcgatga agatcgcgat ttccatattg acatcaatat taaaggcgta
    24601 tggaacgtca cgaaggcggt gctgccagag atgattgcgc gcaaagatgg tcgcattgtg
    24661 atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc gtacgcctta
    24721 acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg tggagtacgc gcagtctggt
    24781 attcgcgtta acgccatttg cccgggatac gtgcgcacac caatggcgga aagcattgcc
    24841 cgccagtcga acccggaaga tccagagtcg gtgctgactg aaatggcgaa agcaatcccg
    24901 atgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct cgcatcggat
    24961 gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac actgccggag
    25021 acggttagcg tcggtatctg attcacctct gtttcctccc tgcatttgtg gggaggattt
    25081 cgttttaaac ccggttctta ttcccttttc aacttccaaa tcaccaaacg gtatataaaa
    25141 ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca ttaaatttat
    25201 aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgcgctg gtcgcttctc
    25261 tgctgctggc gggccatgta caggcaacgg aactgctgaa cagttcttat gacgtctccc
    25321 gcgagctgtt tgccgccctg aatccgccgt ttgagcaaca atgggcaaaa gataacggcg
    25381 gcgacaaact gacgataaaa caatctcatg ccgggtcatc aaaacaggcg ctggcgattt
    25441 tacagggctt aaaagccgac gttgtcactt ataaccaggt gaccgacgta caaatcctgc
    25501 acgataaagg caagctgatc ccggccgact ggcagtcgcg cctgccgaat aatagctcgc
    25561 cgttctactc caccatgggc ttcctggtgc gtaagggtaa cccgaagaat atccacgatt
    25621 ggaacgacct ggtgcgctcc gacgtgaagc tgattttccc gaacccgaaa acgtcgggta
    25681 acgcgcgtta tacctatctg gcggcatggg gcgcagcgga taaagctgac ggtggtgaca
    25741 aaggcaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg ttcgataccg
    25801 gcggtcgtgg cgcaaccacc acttttgccg agcgtggcct gggcgatgtg ctgattagct
    25861 ttgaatcgga agtgaacaac atccgtaaac agtatgaagc acagggcttt gaagtggtga
    25921 tcccgaaaac caatattctg gcggaattcc cggtggcgtg ggtcgataaa aatgtgcagg
    25981 ccaacggtac ggaaaaagcc gcaaaagcct acctgaactg gctctacagc ccacaggcgc
    26041 agaccatcat caccgactat tactatcgtg tgaataaccc ggaagtcatg gacaaactga
    26101 aaaacaaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc tcctggccgg
    26161 aagtgatgaa aacccacttc accagcggcg gcgagttaga caagctgtta gcggcggggc
    26221 gtaattaatg tttgcagttt catcaagacg cgtgctgccg ggctttacct taagcctcgg
    26281 caccagtctg ctgtttgtgt gcctgatttt gctgctgccg ctctccgcgc tggtgatgca
    26341 gctggctcag atgagctggg cgcagtactg ggaggtgatc accaacccgc aggtagtagc
    26401 ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg atttttaacg gcgtttttgg
    26461 cctgctgatg gcgtggatct taacccgcta tcgctttccg ggccgcacgc tgcttgatgc
    26521 gctgatggat ttaccctttg cgctgccaac ggcagtcgcc ggtttaacgc tagcctcgct
    26581 cttttctgta aacggttttt acggtgaatg gctggcgaag tttgatatca aagtcaccta
    26641 tacctggctg gggattgcgg tggcgatggc ctttaccagc attccgtttg tggtacgtac
    26701 cgtgcagccg gtgctggaag agttaggccc ggaatatgaa gaagcggcgg aaacgcttgg
    26761 tgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg gagctttctc cggcgctggt
    26821 ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa tttggcgcgg tgatttttat
    26881 cgccggaaat atcgcgtgga agacggaagt gacgtcgctg atgatttttg tgcgcttaca
    26941 ggagtttgat tacccggcag cgagcgcgat tgcttcggtg atcctcgcgg catctctgct
    27001 gctgctgttc tcaattaaca ctctgcaaag tcgctttggt cggcgtgtgg taggtcatta
    27061 atggcggaag ttacccaatt gaagcgttat gacgcgcgcc cgattaactg gggcaaatgg
    27121 tttctgattg gcatcgggat gctggtttcg gcgttcatcc tgctggtgcc gatgatttac
    27181 atcttcgtgc aggcattcag caaggggctg atgccggttt tacagaatct ggccgatccg
    27241 gacatgctgc acgccatctg gctgacggtg atgatcgcgc tgattgccgt accggtaaac
    27301 ctggtgttcg gcattctgct ggcctggctg gtgacgcgct ttaacttccc tggacgccag
    27361 ttactgctga cgctactgga cattccgttt gccgtatcgc cggtggttgc cggtctggtg
    27421 tatctgctgt tctacggctc taacggcccg ctgggcggct ggctggatga acataatctg
    27481 caaattatgt tctcctggcc cggaatggtg ctggtcacca tctttgtgac gtgtccgttt
    27541 gtggtgcgcg agctggtgcc ggtgatgtta agccagggca gccaggagga tgaagcggcg
    27601 attttgcttg gcgcgtctgg ctggcagatg ttccgtcgcg tcacattgcc gaatattcgc
    27661 tgggcgctgc tttatggcgt ggtactgacc aacgcccgcg cgattggcga gtttggcgca
    27721 gtgtcggtgg tttccggctc gattcgcggc gaaacacttt cgctgccgtt acagattgaa
    27781 ttgctggagc aggactacaa caccgtcggc tcctttaccg ctgcggcact gttaacgctg
    27841 atggcgatta tcaccctgtt tttaaaaagt atgttgcagt ggcgcctgga gaatcaggaa
    27901 aaacgcgcac agcaggagga acatcatgag cattgagatt gccaatatta agaagtcgtt
    27961 tggtcgcacc caggtgctga acgatatctc actggatatt ccttcaggtc agatggtcgc
    28021 gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg cgcattatcg ccgggctgga
    28081 gcatcaaacc agcgggcata ttcgcttcca cggcaccgac gtcagccgcc tgcacgcgcg
    28141 cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg ttccgccata tgacggtatt
    28201 cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc gagcgcccga atgccgcagc
    28261 catcaaagcg aaagtgacaa aattgctgga aatggtccag cttgcgcatc tggcggatcg
    28321 ttatccggcg cagctttccg gcggccagaa acagcgtgtg gcgctggcgc gcgcgctggc
    28381 tgtagaaccg caaattctgc tgcttgatga accgtttggc gcgctggatg cgcaggtgcg
    28441 taaagagctg cgtcgctggc tgcgtcaact gcatgaagag ctgaaattca ccagcgtgtt
    28501 tgtgacccac gaccaggaag aggcgaccga agtagccgat cgtgtagtgg tgatgagtca
    28561 ggggaatatt gaacaggctg atgcgccgaa tcaggtatgg cgcgaaccag cgacccgttt
    28621 tgtactcgaa tttatgggcg aagtgaaccg cctgcaggga accattcgcg gcgggcagtt
    28681 ccatgttggc gcacatcgct ggccgctggg ctacacacct gcttatcagg ggccggtgga
    28741 tctcttcctg cgcccgtggg aagtggatat cagccgccgt accagtctcg attcgccgct
    28801 gccggtacag gtactggaag ccagcccgaa aggtcactac acccaattag tggtgcagcc
    28861 gctggggtgg tacaacgaac cgctgacggt cgtgatgcat ggcgacgatg ccccgcagcg
    28921 tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg tataacggcg acgagcgtat
    28981 cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc tgataggttg agtgaatgtt
    29041 aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat ggcaaggttt gtaacctgta
    29101 gacctgataa gacgcgcaag cgtcgcatca ggcaacacca cgtatggata gagatcgtga
    29161 gtacattaga acaaacaata ggcaatacgc ctctggtgaa gttgcagcga atggggccgg
    29221 ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa cccggcaggt tcggtgaaag
    29281 atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg cggggaaatt aaaccgggtg
    29341 atgtcttaat cgaagccacc agtggtaaca ccggcattgc gctggcaatg attgccgcgc
    29401 tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat gagccaggaa cgccgtgcgg
    29461 cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa agagcagggc atggaaggtg
    29521 cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg aaagctgctc gatcagttta
    29581 ataatcccga taacccttac gcccattaca ccactaccgg gccggaaatc tggcagcaaa
    29641 ccggcgggcg cattacccat tttgtctcca gcatgggaac aaccggcact atcaccggcg
    29701 tttcacgctt tatgcgcgaa caatccaaac cagtaacgat tgttggcctg cagccggaag
    29761 aaggcagcag tattcccggc attcgccgct ggcctgcgga atatctgccg gggattttca
    29821 acgcttctct ggtggatgag gtgctggata ttcatcagcg cgatgcagaa aacaccatgc
    29881 gcgaactggc agtgcgggaa ggaatattct gtggcgtcag ctccggcggc gcggttgccg
    29941 gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt ggtagcgatc atctgcgatc
    30001 gtggcgatcg ctacctttcc accggggtgt ttggggaaga gcattttagc cagggggcgg
    30061 ggatttaaga ttattgcgca ggcaccgttt tatccgtctg cgcaattagc gcatgtaaaa
    30121 aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac aatatacggc ttcccggtaa
    30181 tcgatgacga cgaagcaacc ttatcaataa actgctcggc agtgtcaatg cggttacggg
    30241 tattgcccag cttcagacgc agatgagaaa ccgcttcatc gcaggtatgt tcatcaccgt
    30301 tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc cagcatggcg ttgatgcggg
    30361 cttcttcatg ggcagttaac ttcgcgtaag cggggagtgt cattagtagt gtaatgacca
    30421 gacaaatgat tttcttcatt gaattacctt atccttgttt tgtgatagcc gatttgactg
    30481 ccagaatcag gtaaagtttc ggttggaata cgatgaggat catcggcaag gagaaaaaat
    30541 taaatgagta gtttgttgtt gtttaacgat aagagcaggg cgctgcaggc ggatatcgtc
    30601 gccgtgcagt cgcaggtggt ttacggcagc gtgggcaaca gcattgccgt gcctgctatc
    30661 aaacagaacg gtctgaatgt ctttgccgtg ccgacggtat tgctgagcaa tacgccgcat
    30721 tatgacactt tctacggtgg tgcgattccg gacgaatggt ttagcggcta tttgcgtgcg
    30781 cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta tatgggaacg
    30841 gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga ccatcctgac
    30901 ctattgatca tggtcgatcc ggtgattggc gatattgata gcggaattta tgtcaaacct
    30961 gaccttcccg aggcgtatcg acaatattta ctgccgctgg cgcagggaat tacccccaat
    31021 atctttgagc tggaaatcct gaccggtaaa aattgccgcg atctcgacag tgccattgct
    31081 gccgcaaaaa gtctgctttc agacacatta aaatgggtgg tgattaccag cgcctccggt
    31141 aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt gaatgtcatt
    31201 tcccattcac gggtaaaaac cgacctgaaa gggactggcg acctgttttg tgctcagctt
    31261 atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc ggggttgcgc
    31321 gtactggaag tgatgcgcta cacccagcag catgagagcg atgaattgat tttgccgccg
    31381 ctggcggaag cataaaaaat ggcgccgatg ggcgccattt ttcactgcgg caagaattac
    31441 ttcttgatgc ggataaccgg ggtttcaccc acggttacgc taccggacag tttgatcagt
    31501 tctttgattt cgtccatgtt ggagataaca accggagtca gggtagactt ggctttctct
    31561 tccagcagcg gcagatcaaa ttcaatgaca gtatcgccaa ctttcacgcg ctgaccttct
    31621 tcagcaatac gcttgaagcc ttcgcctttc agttcaacgg tgtcgatacc gaagtggacg
    31681 aacagttcaa cgccgctatc agattcgata gagaacgcgt ggttggtttc aaagatttta
    31741 ccaatggtgc cgtctacagg cgcgaccatt ttgttacccg ttggtttgat agcaatacca
    31801 tcaccaacga ttttttccgc aaaaacgaca tccggcacgt cttcgatatt gacgatctcg
    31861 ccagagagcg gagcaatgat ctcaatagtt ccggtatcct tcttgtcgtc ggaaaccaga
    31921 gatttcagtt tatcgaacaa acccatgatc ttctcctaag cagtaaattg ggccgcatct
    31981 cgtggattag cagattgttt tttcttcaat gaacttgtta accagcgtca ttaactcgtc
    32041 cgttgtcggt tgagcaagag cctgctctgc taacaccttc gcatcttcga agttcgtgtt
    32101 acggataatc ttcttaatgc gcgggataga aatggcgctc atagagaatt cgtccagacc
    32161 catccccagc aacagaagtg tagcacgttc atcgccagca agctcaccac acatgccagt
    32221 ccatttgcct tcagcatgag aagcatcaat aacttgcttg atcaagttca gcacggacgg
    32281 tgacattggt tggtaaaggt gtgaaatcat atcattacca cggtcaactg ccagagtgta
    32341 ctgcgttaaa tcattggtgc cgatactaaa gaaatcaact tctttggcta aatgacgtgc
    32401 aattgttgcg gcagccggtg tttccaccat tacgccgatt tcaattgact cgtcaaacgc
    32461 tttaccttcg tcgcgcagtt cctgtttgta gatttcgatc tctttgcgca gtgcacgcac
    32521 ttcttcaaca gagatgatca tcgggaacat aatgcgcaat ttaccgaaag ccgaggcacg
    32581 caggatagcg cggagctgat cgcgcaggat ctctttacga tccatcgcga tacggatagc
    32641 gcgccagccg aggaacgggt tctcttcttt cgggaagttc atgtatggca gctctttgtc
    32701 gccgccgatg tccatggtac gaacgataac cgcctgcgag ccacacgctt cagccactgc
    32761 tttgtaagca gcaaactgtt cttcttcagt gggcagcgcg tcgcggtcca tgaacaggaa
    32821 ctcagtacga tacagaccaa cgccttcagc gccgttacgc tctgcacctt caacgtcacg
    32881 aaccgtacca atgttagcgc acacttctac ctggtgaccg tccagcgtaa tagctggcag
    32941 atctttcagt ttagcaagct ctgctttttc agaagccact tgctcctgaa cagcgcgcat
    33001 tttatcaata acttcgttgg ttggattgac gtaaacctga ttatttacgg catccagaat
    33061 cagatagtcg tcatttttca cctgagaggt gacgctaccg gtacccacga tagcaggcag
    33121 ttccagagaa cgcgccatga tagaggtgtg ggaagtacgg ccacccgcgt cggtgatgaa
    33181 acccagcacc ttcttcaggt tcagctgtgc ggtttcggac ggcgtcaggt cagcggcaac
    33241 cagaatgact tcatcctgaa tggcgctcag gtcgataatc ttcaggccca ggatgttgcg
    33301 cagcaggcgc ttaccgatat cacgtacgtc agccgcacgt tctttcaggt attcatcatc
    33361 cagctcttcc agggcagaag cctgaccttc gataacttca tgagcagctg cgtcagctgt
    33421 catgtgctta tctttaatca gggctatgat ttcctgctcc agctcctcat cttcgagcag
    33481 cataatatgc ccttcaaaga tggcttcttt ttcttcaccg aacgtttcac cagctttcgt
    33541 tttgatcgtt tccagctggg ctgatgcctt ggcacgaccg ctcagaaaac gttcaacttc
    33601 ctgatcaacc tggtcggcag aaattttttt ccggtcaatg acaatttcgt cttctttcag
    33661 aagcagagct ttaccgaaag cgatacccgg ggatgctaaa atgcctgaaa tcataaccct
    33721 accttacttg tgactgattt ttaaaagaac ccgggaaatt actcgagttc cgccatcagt
    33781 ttaaccagat gttcaaccgc tttctgctcg tcttcgcctt ctgcggagat agtcacaacg
    33841 gtaccttgag tcaggcccag agtctgcagt ttaaacaggc ttttcgcgct ggcgcttttg
    33901 ccgttggaag tcacagtaat ttcagaagtg aagcccttag cttcttttac aaactgggca
    33961 gcagggcggg tgtgcagacc gttcggagcg gtaatggtaa cttcttgctg gaacattgta
    34021 tttccccaac ttataggttt agtgttgtgg aactaaagtc tagcctggcg gttcgacttt
    34081 agcctgtatt gttagcgcca gtttttaaca gacgcgacgc acgaagagcg caatacagtc
    34141 cattgctggc gggtgttgac cgctgacgtt tcgtacgtca ttaaacatta tgccgctaaa
    34201 ggaagaattg aaccaaatca taaaatcgat tcagctttgg cttttcctgt aacgattaat
    34261 ttcgcgcatc aaaataatta gtctggttaa ataccagttc cgacaggctg aatcaatgcc
    34321 aggaggggcg aaagtttgaa gcaggccaca aaaaagcacc taaaaaggtg cttttttacg
    34381 catttttaac aagctggcat tactgttgca gttctttctc agtgaagaga tcggcaaaca
    34441 atgcggtgct taaataacgc tcacccgatg acggtagaat aaccacaata ttcttgttgg
    34501 taaagctttc atcttcttgt agtttcaacg ccgcagcaac agctgctcca gaagagatac
    34561 ctgcaagaat accttcttct tccatcagac gacgtgcggt agaaatcgct tcttcattgg
    34621 tgatgccaat gactttatcg accagcttga gatcgaggtt agctgggata aaaccagcgc
    34681 caataccctg aattttatgc gggccaggtt taatctcttc accagccagt gcctgggcga
    34741 taactggaga atcggttggc tcaacggcga cagagataag atcggtcttg cctttggtgc
    34801 ctttaatgta gcggctgacg ccagtcagcg taccgccagt cccaacgcca gcaataaata
    34861 catcaacctg accgtcggta tcttcccaga tctccggacc ggtggttttt tcgtgaattt
    34921 cagggtttgc cggattgctg aattgttgca gcagcaggta tttctctgga ttgctggcga
    34981 caatttcttc tgctttttgg attgcgcctt tcatgccttt agcaccttcc gtcagcacca
    35041 ggtttgcacc taacgctttc agcagcttgc ggcgttcaat actcatggtt tctggcatgg
    35101 tcagggtgag tttgtaaccg cgagcggcag ctacataggc cagtgcaatc ccggtattac
    35161 cgctggtcgg ttcaaccagt tcaacgcctg gtttcagcac gccgcgcttt tcggcatccc
    35221 aaatcatgtt ggcaccgata cggcacttaa cgctgaagct ggggttacga gattccacct
    35281 tcgccagaat gcgtccgtta ccgatgcgat tcaggcgaac cagcggcgtg tgaccgatag
    35341 tcagcgagtt atcttcaaaa atcttactca tggcctgtcc ttaactgtat gaaattggga
    35401 tacaacaggt agcatacccg ctcagagaat atgcggaagt aaggatttag catatctata
    35461 tacagaaggg aaataatgac atgcaagatg gaataagggg cggcataagc cacccctgtt
    35521 tcacacaaac ggtttacaaa ataaccggta gattgttacc gccacatcgc gtgtttatcg
    35581 cgatagcaat cgacccacat cgccgtcgcg ccacaaacgg caacgggcat gatgaacaga
    35641 ttaagcagcg ggatcatcgt aaacaggctg gttaaagcac caaactgcat attggtgatt
    35701 ttgcgtgtgc gcagggcggt gcgcatctct ttaaacggca ctttgtggtt atcgaagggg
    35761 taatcgcaat actggatggc taacatccag gcgctaaaca ggaaccacag taccggcgcg
    35821 acggtttgcc caataccagg gattaagtaa agaattagca atacaattgc gcgcggcaga
    35881 taccaggcaa atttttgcca ttcgcgtttc atgattcgcg gcacatcttt catgataccg
    35941 aaaatcccgg tatctggcgg tgtagcgcca gtcaatcgtg cttccagttg ttcagccaat
    36001 aaaccgttaa acggagcggc aatccagtta gcaatcgtgg agaagaaata gccaaacact
    36061 aacagcacag agatgacagc cagaggccac aacagataac tcagccattg tagccagtcc
    36121 ggaacgtaac tcatgagagt cgggatccag acatcgagct gtgtaaagag ccaccagaat
    36181 gcgcccccca tcaacaaaat attgaccagc agcggtaaaa taacgaaacg ccgaatccca
    36241 ggttgcgaga cgagcttcca gccttgcgca aaatagtaaa aaccgctgcg tggggcagat
    36301 gtgaatgatg aaaccataat caggatgagc tccttttgac caatcccagg aaaattctgc
    36361 gtattttacc gggtaattgc gcaatggaca gttaggatat gttcgaaaaa acagcaaaaa
    36421 gcacgatttc atctatcttt gtgctgtgaa agttaatagt gcacttgcac ttgaggtaat
    36481 cggcaaatac tcttagtgag taaatgtttg ccgtggtggc aaggtgttag aacaacagag
    36541 aatataatga tgcaggattt gcgtctgata ttaatcattg ttggcgcgat cgccataatc
    36601 gctttactgg tacatggttt ctggaccagc cgtaaagaac gatcttctat gttccgcgat
    36661 cggccattaa aacgaatgaa gtcaaaacgt gacgacgatt cttatgacga ggatgtcgaa
    36721 gatgatgagg gcgttggtga ggttcgtgtt caccgcgtga atcatgcccc ggctaacgct
    36781 caggagcatg aggctgctcg tccgtcgccg caacaccagt accaaccgcc ttatgcgtct
    36841 gcgcagccgc gtcaaccggt ccagcagccg cctgaagcgc aggtaccgcc gcaacatgct
    36901 ccgcgtccag cgcagccggt gcagcagcct gcctatcagc cgcagcctga acagccgttg
    36961 cagcagccag tttcgccaca ggtcgcgcca gcgccgcagc ctgtgcattc agcaccgcaa
    37021 ccggcacaac aggctttcca gcctgcagaa cccgtagcgg caccacagcc tgagcctgta
    37081 gcggaaccgg ctccagttat ggataaaccg aagcgcaaag aagcggtgat tatcatgaac
    37141 gtcgcggcgc atcacggtag cgagctaaac ggtgaactgc ttcttaacag cattcaacaa
    37201 gcgggcttca tttttggcga tatgaatatt taccatcgtc atcttagccc ggatggtagc
    37261 ggcccggcgt tattcagcct ggcgaatatg gtgaaaccgg gaacctttga tcctgaaatg
    37321 aaggatttca ctactccggg tgtcaccatc tttatgcagg taccgtctta cggtgacgag
    37381 ctgcagaact tcaagctgat gctgcaatct gcgcagcata ttgccgatga agtgggcggt
    37441 gtcgtgcttg acgatcagcg ccgtatgatg actccgcaga aattgcgcga gtaccaggac
    37501 atcatccgcg aagtcaaaga cgccaacgcc tgatacactt aaggcaaatt aactcctctt
    37561 cgaacccccg cttgtcgggg gtttttagca ttgatggtgc gatatggaat caatcgaaca
    37621 acaactgaca gaactgcgaa cgacgcttcg ccatcatgaa tatctttatc atgtgatgga
    37681 tgcgccggaa attcccgacg ctgaatacga caggctgatg cgcgaactgc gcgagctgga
    37741 aaccaaacat ccagaactga ttacgcctga ttcgcctacc caacgtgtag gcgctgcgcc
    37801 gctggcggct ttcagccaga tacgccatga agtaccaatg ctgtcactgg ataacgtttt
    37861 tgatgaagaa agctttcttg ctttcaacaa acgtgtgcag gaccgtctga aaaacaacga
    37921 gaaagtcacc tggtgctgtg agctgaagct ggatggcctt gccgtcagta ttctgtatga
    37981 aaatggcgtt ttagtcagtg ccgcgacccg tggcgatggc accaccgggg aagatatcac
    38041 gtctaatgtg cgtactattc gcgctattcc gctgaagctg cacggagaga atatcccggc
    38101 gcgtctggaa gtgcgtggtg aagtgttcct gccgcaggcg gggttcgaaa agattaacga
    38161 agatgcgcga cgcacgggcg ggaaagtttt tgctaaccca cgtaatgcgg cagctggttc
    38221 actgcgtcag cttgatccgc gtattacagc gaagcgaccg ctcacttttt tctgctatgg
    38281 cgttggtgtt ctggaaggtg gcgagctgcc ggatactcat cttggccgtt tactgcaatt
    38341 taaaaagtgg gggttgccgg tcagcgatcg ggtaacgctt tgtgaatcgg cggaagaagt
    38401 gctggcgttc tatcacaaag tggaagaaga ccgcccgacg ctgggctttg atatcgacgg
    38461 cgtggtgatt aaggtcaact cactggaaca gcaggagcag cttggctttg tcgcgcgtgc
    38521 cccgcgctgg gcggtagcgt ttaaattccc ggcgcaggag cagatgacct ttgtgcgtga
    38581 cgtcgagttt caggttgggc gtactggcgc gattacgcct gttgcgcgtc tggaacctgt
    38641 ccatgttgct ggcgtgctgg tgagtaacgc aaccttacac aatgcggatg aaatcgaacg
    38701 tcttggttta cgcattggcg ataaagtggt gattcgccgc gctggcgacg tgatcccgca
    38761 ggtggttaac gtcgtgcttt ctgaacgccc ggaagatacc cgtgaggttg tattcccgac
    38821 gcattgtccg gtatgtggtt ctgacgttga gcgtgtggaa ggtgaagcgg ttgcccgctg
    38881 taccggtggc ctgatttgcg gagcgcagcg taaagagtcg ctgaaacact ttgtttcccg
    38941 ccgtgcgatg gatgttgacg gaatgggcga caaaatcatc gatcagctgg ttgaaaaaga
    39001 atatgtccat actccggcag atctgttcaa actcaccgca ggcaaactga ccggactgga
    39061 gcgtatgggg ccaaaatcgg cacaaaacgt ggttaacgcg ctagaaaaag cgaaagaaac
    39121 cacctttgct cgcttcctct atgcacttgg catccgtgaa gtcggcgagg ccaccgcagc
    39181 aggtctggcg gcatatttcg gcacgctgga agcgctggaa gccgcttcga ttgaagagct
    39241 gcaaaaggtg cctgatgttg gcattgtcgt tgcatcccac gttcacaact tctttgccga
    39301 agaaagcaac cgcaatgtca tcagcgagct gttggcggaa ggtgttcact ggcctgcgcc
    39361 gatcgttatc aacgcggaag agattgacag cccgtttgct ggtaaaaccg tggtgcttac
    39421 gggcagctta agtcagatgt cacgtgatga cgctaaagct cgactggtcg aactgggcgc
    39481 gaaagtcgcg ggcagcgtgt cgaagaaaac cgatctggtg atagcgggtg aagctgcagg
    39541 atctaaactg gcgaaggcgc aggaactggg cattgaagtc atcgacgaag cggaaatgct
    39601 gcgtttgctg ggtagctgag atggaaaaag agcagctgat tgaaatagcc aatacgataa
    39661 tgccgtttgg caaatacaaa gggcgtcgct taatcgacct gccggaggag tatctgctgt
    39721 ggtttgcccg caaagatgag ttcccggcag ggaagctcgg tgagctaatg caaatcacgc
    39781 tgctgattaa aaccgagggg ctgacgcaac tggtccagcc cctgaagcgt ccgctttaag
    39841 ctttatcggt gctgctttcc tgctgcgcct gtaactgttc ggtctggcgt ttgtatcgac
    39901 gcgccagcac cgcacagacc atcaattgga tctgatggaa aatcatcagg ggcagcacca
    39961 tcataccgat caccgatgtg gggaacagaa tgtttgccat cgggatgcca tttgccagac
    40021 tctttttcga accacaaaag acgatagtaa tttcatctgc cttattgaag cccagtcggc
    40081 gtgccataaa gacgttaact acaatcacga tagccagaag aacgcagctg accaccacga
    40141 taaacagcaa tgatccccag ccaactttat gccagatacc attaacgacg gcttcgctga
    40201 acgctgtata aaccaccaac agaatggacg tctggtcagt tttcgcaatc cattttttat
    40261 tgcgcgacac ccagtcacca atccacggcc gggaaagatg ccccaacaca aacggcagca
    40321 gcagttgcag cataatttta ccgacctgct caaggctgcc ccctgcaccg tgaacattca
    40381 tcaccagacc aaccagcaat ggtgaaagga aaatccccag caggctggat gctgacgcag
    40441 aacaaaccgc cgccgcgacg ttaccgcccg ccattgacgt gaaggcgatt gcagactgca
    40501 cggtagccgg gagaatgcac aagtagagaa aaccggagta gagcatcggg tcgacattta
    40561 ccggtttcca ccaggcaaac agtacaccca gaatcggaaa cagcacgaag gtgctgcaaa
    40621 ttacccacaa atgcagtcgc cagtgaccac cgccagcaat aatcgcctca cgcgacaact
    40681 tcgcgccgtg cataaagaac agcagggcaa tcgctgcggt ggtcagattt tcaaagaagg
    40741 ggacgaaatc gcctctggcc ggaaagaaag aggccagcaa caccaccgtg atcagggtta
    40801 aggtgaaagg atcgaggata cgaaaaagtt tcataaagac tccagatgat cgatggttct
    40861 attgtgcttc ttccagtttg agaaataaaa ttgatttatt
//
LOCUS       DAZFWP020000019        40788 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFWP020000019 DAZFWP020000000
VERSION     DAZFWP020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.019"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            69..1829
                     /gene="tssF"
                     /locus_tag="ACHK0Z_RS04855"
                     /old_locus_tag="pgaptmp_000971"
     CDS             69..1829
                     /gene="tssF"
                     /locus_tag="ACHK0Z_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4501036.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGSLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCFYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPFYLNADSPVACALHRALTLGTQQFWLRLPGQDRRTLDAHFS
                     PLGFEDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLELVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPPEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDADRMLEDETLSLSLTGTNGHLPRKALQSTLLDRPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            1793..2872
                     /gene="tssG"
                     /locus_tag="ACHK0Z_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             1793..2872
                     /gene="tssG"
                     /locus_tag="ACHK0Z_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4501037.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQFLEQSQQNAPLGST
                     DNPATDAVRFRPHPGMGFPVSELKNVERDAGNPNVPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAMDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPALSIRNRESMTHVSYSF"
     gene            2853..3389
                     /gene="tssJ"
                     /locus_tag="ACHK0Z_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             2853..3389
                     /gene="tssJ"
                     /locus_tag="ACHK0Z_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4501038.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            3393..3821
                     /gene="tssE"
                     /locus_tag="ACHK0Z_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             3393..3821
                     /gene="tssE"
                     /locus_tag="ACHK0Z_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4501039.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMVAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     YLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            3821..5197
                     /locus_tag="ACHK0Z_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             3821..5197
                     /locus_tag="ACHK0Z_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HGU4501040.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     RVEKLSLALFRQNGVELQTQVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMKQPEKSEESPQPVFATRPAQQNDK
                     DASPSAPSPEISWQRTWPIFMAGMVVMACLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSTEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            5262..5810
                     /locus_tag="ACHK0Z_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             5262..5810
                     /locus_tag="ACHK0Z_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HGU4501041.1"
                     /translation="MYKKERNRPFETREEVLAYHKREKIPCLECGKLLIFLPRHIWFV
                     HGIRADEYRVKWNIPKHIALAGISYLEKQSEYMKARIAKGKLDPAEQIAMMRIKGRQL
                     KSDPDWRNRSGSILKKQYARRDILKNRVWERSPSIKSVSVELKTEAIRRMKNRKVVGE
                     KVQDIADDLNVSISRLYHWLKM"
     gene            complement(6156..7409)
                     /gene="amiC"
                     /locus_tag="ACHK0Z_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             complement(6156..7409)
                     /gene="amiC"
                     /locus_tag="ACHK0Z_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU4501042.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            7641..8972
                     /gene="argA"
                     /locus_tag="ACHK0Z_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             7641..8972
                     /gene="argA"
                     /locus_tag="ACHK0Z_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU4501043.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(9034..10860)
                     /gene="recD"
                     /locus_tag="ACHK0Z_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             complement(9034..10860)
                     /gene="recD"
                     /locus_tag="ACHK0Z_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU4501044.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(10860..14402)
                     /gene="recB"
                     /locus_tag="ACHK0Z_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             complement(10860..14402)
                     /gene="recB"
                     /locus_tag="ACHK0Z_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU4501045.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSVGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(14395..17283)
                     /gene="ptrA"
                     /locus_tag="ACHK0Z_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             complement(14395..17283)
                     /gene="ptrA"
                     /locus_tag="ACHK0Z_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU4501046.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(17459..20827)
                     /gene="recC"
                     /locus_tag="ACHK0Z_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             complement(17459..20827)
                     /gene="recC"
                     /locus_tag="ACHK0Z_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU4501047.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCHEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(20840..21163)
                     /locus_tag="ACHK0Z_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             complement(20840..21163)
                     /locus_tag="ACHK0Z_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU4501048.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(21148..21555)
                     /locus_tag="ACHK0Z_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             complement(21148..21555)
                     /locus_tag="ACHK0Z_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU4501049.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVINGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(21552..22115)
                     /locus_tag="ACHK0Z_RS04925"
                     /old_locus_tag="pgaptmp_000985"
     CDS             complement(21552..22115)
                     /locus_tag="ACHK0Z_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4501050.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(22106..22576)
                     /locus_tag="ACHK0Z_RS04930"
                     /old_locus_tag="pgaptmp_000986"
     CDS             complement(22106..22576)
                     /locus_tag="ACHK0Z_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4501051.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(22761..23555)
                     /gene="thyA"
                     /locus_tag="ACHK0Z_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             complement(22761..23555)
                     /gene="thyA"
                     /locus_tag="ACHK0Z_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU4501052.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(23562..24437)
                     /gene="lgt"
                     /locus_tag="ACHK0Z_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             complement(23562..24437)
                     /gene="lgt"
                     /locus_tag="ACHK0Z_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU4501053.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(24588..26834)
                     /gene="ptsP"
                     /locus_tag="ACHK0Z_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             complement(24588..26834)
                     /gene="ptsP"
                     /locus_tag="ACHK0Z_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4501054.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(26847..27377)
                     /gene="rppH"
                     /locus_tag="ACHK0Z_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             complement(26847..27377)
                     /gene="rppH"
                     /locus_tag="ACHK0Z_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU4501055.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(27730..27876)
                     /gene="ygdT"
                     /locus_tag="ACHK0Z_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             complement(27730..27876)
                     /gene="ygdT"
                     /locus_tag="ACHK0Z_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU4501056.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            28062..28751
                     /gene="mutH"
                     /locus_tag="ACHK0Z_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             28062..28751
                     /gene="mutH"
                     /locus_tag="ACHK0Z_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU4501057.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDIIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            28820..29533
                     /locus_tag="ACHK0Z_RS04965"
                     /old_locus_tag="pgaptmp_000993"
     CDS             28820..29533
                     /locus_tag="ACHK0Z_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4501058.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            29671..29889
                     /gene="ygdR"
                     /locus_tag="ACHK0Z_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             29671..29889
                     /gene="ygdR"
                     /locus_tag="ACHK0Z_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU4501059.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            29997..31037
                     /locus_tag="ACHK0Z_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             29997..31037
                     /locus_tag="ACHK0Z_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU4501060.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(31069..32262)
                     /gene="lplT"
                     /locus_tag="ACHK0Z_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             complement(31069..32262)
                     /gene="lplT"
                     /locus_tag="ACHK0Z_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU4501061.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(32255..34414)
                     /gene="aas"
                     /locus_tag="ACHK0Z_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             complement(32255..34414)
                     /gene="aas"
                     /locus_tag="ACHK0Z_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU4501062.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            35000..36031
                     /gene="galR"
                     /locus_tag="ACHK0Z_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             35000..36031
                     /gene="galR"
                     /locus_tag="ACHK0Z_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU4501063.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(36038..37300)
                     /gene="lysA"
                     /locus_tag="ACHK0Z_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             complement(36038..37300)
                     /gene="lysA"
                     /locus_tag="ACHK0Z_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU4501064.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGLVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            37422..38357
                     /gene="lysR"
                     /locus_tag="ACHK0Z_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             37422..38357
                     /gene="lysR"
                     /locus_tag="ACHK0Z_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU4501065.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(38344..39036)
                     /gene="ygeA"
                     /locus_tag="ACHK0Z_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             complement(38344..39036)
                     /gene="ygeA"
                     /locus_tag="ACHK0Z_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU4501066.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIDECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(39165..40583)
                     /gene="araE"
                     /locus_tag="ACHK0Z_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             complement(39165..40583)
                     /gene="araE"
                     /locus_tag="ACHK0Z_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU4501067.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         9928 a        11118 c        10271 g         9471 t
ORIGIN      
        1 aaaataaaaa attctaatct cactactcgt tggtattaat ttcaataaaa ggaaagataa
       61 atcctccgat ggacgactta acccaacgct actacgaagc cgaaatgcgc tacctgcgtg
      121 aagcggggaa agaatttgcg caggcttacc ccgaccgcgc cgcgatgctc aatcttgata
      181 aacccggcgc acgcgatccc tatgtggaac gcctgttcga aggctttgct tttttaatgg
      241 gcagcctgcg cgagaagctg gacgacgatc tgccggagct gactgaaggg ctggtcagcc
      301 tgctgtggcc tcactacctg cgaactatcc cgtcactttc cgtggttgaa ctgtcgacag
      361 atcaccggca gatgaagcag agcgaaacgc tgagcgattt tcaggtattg tcgcgccctg
      421 ttggcgagcg cagaacccgc tgtttctaca gcgccacgcg ggatatcacg ctgcatccgc
      481 tggcgttgcc ggacgtgtca ttgcaatatg aaccggacgg acgttcggtt attcgtctgc
      541 gctttgaatg cggcccgctg gtcggtgact ggtcacagat cgatttatcc cgcctgccgt
      601 tctatctcaa tgccgacagt ccggtggcct gtgcgctgca ccgggcttta acgcttggta
      661 cgcagcagtt ctggttgcgt ctgccggggc aggatcgccg gacgcttgac gcacactttt
      721 ccccgttggg ctttgaggat aatgacaggc tctggccaaa aggcgagtcg gcgttcagcg
      781 gctaccagtt gctgctggag tatttcacct tccgcgagaa gtttatgttt gtggcactca
      841 acgggcttga gctggttgca tggccggagg ggataaccgg ctttgagatt gacgtggtgc
      901 tgaacgagaa ctggcctcac gacctgccgt ttgacagcga taatatccgt ctgcactgcg
      961 tgccggtcat caatctgttc ccgcccgaag ccgatccgtt acacctttcg ccactggaaa
     1021 acgaatttct gctacgcccg atgcgcattc aggacgggca taccgagatt tattccgtcg
     1081 acaacattat ttcttcgcgc cataccggca gtcaggcgta tgtccccttc tccagctttc
     1141 gtcaccgggg cgggatgctg cgccacgatg cgccggaacg ctattaccac acgcgggtaa
     1201 agcgcggccc ttccggtctg catgacacct ggctgattct gggcggtgat gcgtttgatg
     1261 ctgaccggat gctggaggat gaaacgctct ctttaagcct gaccggtact aacgggcatc
     1321 ttccccgtaa ggctttacag agcacgctgc tcgataggcc agttcatgcc tcacagaacg
     1381 tcctgcgggt gcgcaacctg tgcgccccaa cgcagccctg ctatcccccg gcgcgtgacc
     1441 gcttccactg gcgggtgctc agccaccttg gttctaactt tttatcgatg atggataacg
     1501 ccgaaattct gcgcggaacg ctggcgctgt acgactggac cgaaagcgag atgaaccgcc
     1561 gtcgtctgga ggcgattgtc gacgttcagc acagcctgat acagcgtttt gaaagaggct
     1621 ttctgctgcg tggcgtggat attcaggtga cgctggacag taacggcttt gccggagaag
     1681 gtgatatcac gctgtttggt gaactgctgc accgcttttt cgcactgtat gccgatatcc
     1741 acttattcac tcaactgacc ttaattctgc aacctacagg aaagtgcctg caatggacag
     1801 agcatcacag ccagcgcgta ccgggctgac cgaccggctg gccccggata ttgcgcggat
     1861 caatttttac cgtttttgcc agtttctgga acagagccag cagaacgcgc cgctgggcag
     1921 taccgacaac cccgccaccg atgcggtgcg ctttcgtccc catcccggaa tgggctttcc
     1981 ggtcagcgag ttaaagaacg ttgaacgtga cgcgggcaat cccaacgtgc cacccacggt
     2041 gcgcaccacc tttctcggcc tgtacggcgt ggattcgccg ctgccaaccg cctatctgga
     2101 ctacattacc cagcgtcatg acggacatga cgcagtgatg gcgtttctcg atatttttaa
     2161 ccatcgcttt atcacccagt attaccgcat ctggcgcaaa tataactacc cggcgtcgtt
     2221 tgaagcaggt gctatggatg acatttcccg ttgtctgctg ggactcatcg ggctgggtat
     2281 tcccggcagc gagaaccata tcgccacgcc ggtatcccgt tttctggccc tgctcagcgt
     2341 gatgcgcctg ccgacgcgca cggcggaagg cgtcacggca ctggtgggac tgcttgcccc
     2401 gctgacgaaa gcgacggtgg tgcctcacga tccgcagcct gttattctcc cggcccctgc
     2461 gggtctgtca aaaaattcac gcatcagcct gaaaacgcga acgctgctgg ggcgtaccgg
     2521 caccgacgtc aacagccagt tgctgctgaa actgtatacc gaagatgccg cagaagcacg
     2581 tggctggcta cctggcgggc agctgcacag cgatttgctg gtgctgctgc gcgtttatct
     2641 gggctggcgc tgtcaggccc gtctgcaact gacgctgccc gtcagcctgt tgcctgctgc
     2701 ccggctggga aaacagcgcg tacagattag ccgtaccggc atccttcgcg cctcctttgc
     2761 cgcgcccgcg accggaacag tgacggtcag tctggggcgt tatcagggat taatacccgc
     2821 tttatccatt cgtaacaggg agagtatgac tcatgtttcc tattcgtttt aaacgtccgg
     2881 cgctgctctg tatggcgatg ctgacggttg ttctgagcgg ctgcgggctg attcagaaag
     2941 tggtggatga atcgaaaagc gtggcctcag ccgttttcta caagcaaatc aaaatactgc
     3001 atctcgattt cttctcccga agtgccctga atacggatgc ggaagatacg ccgctttcca
     3061 cgatggtgca tgtctggcaa ctgaaaaccc gcgaagattt tgacaaggcg gattatgaca
     3121 ccctgtttat gcaggaagag aagacgctgg agaaggacgt actggcaaaa cacaccgtct
     3181 gggtaaaacc ggaaggcacg gcatccctga atgtgccgct ggataaagag acgcagtttg
     3241 tcgccattat tgggcagttt tatcaccctg atgaaaaaag cgacagctgg cgtctggtga
     3301 tcaaaaggga cgaactggag gccgacaagc cgcgctcgat tgaactgatg agaagcgacc
     3361 tgcgactgct gcctctcaag gataaataac cgatgtcgac accttcctta tatgaaatgc
     3421 tgacgttcag tttcagcggc gagttaccac tggaacaggt cagcgagcgg gatcagctta
     3481 tcctttccgt gatggacaat atgcaacgca ttatcaactg tcgggcaggc acactggcgc
     3541 atttgccgga ttacggtctg ccggatctca gtctgatcca tcagggtatg gttgcgggta
     3601 ttcatggcct gatgcgtcag attgaagaaa cgctgctgcg ttatgaacca cgcctgagtc
     3661 agatacaggt ggagttactc ccccagcccc gtccggggta tcttaattac ctgatccacg
     3721 cgcagcttcc cgataccggc tggatacgct ttgatggcgt gttttctccg gaaggacgaa
     3781 ttgttctgcg tcatctcaaa cagcaggagc gggcgtactg atggcaagta acgcgaattt
     3841 tatcagccag ttcgtcatgg gcggcgatcc ctgtacttat aaggaatccg gtgaactaca
     3901 ggctgaaatg agtaaactga ctcacccggc ccgacctgat gtggactggc gtcgggtgga
     3961 aaaactcagc ctcgcgctgt tccggcaaaa tggcgtggaa ttacagacgc aggtctgtta
     4021 cgtactggcg ataaccagac ggcaggggct ggcagggatg gcagacggac tcggttcact
     4081 ggacatactg ctccagcgct gggctgactt ctggccggta caggtacatt ccagaatatc
     4141 actgctcagt tgggtcacag aaaaaatgca gcaggcactg agaacgctgg atattcagta
     4201 tcaggatctg ccgcagattt accgttgtgt acagcatctt tccgccatcg aaaccacgct
     4261 gcaacagtgt gaactgtggc atatgacgaa actggatgtg ctggccgggc agtttcgtaa
     4321 tacggcgcta cgtctggagc ggctggcacc acagggagcg gaaaccacta tcactccccc
     4381 tgaattaccc cgccgggaaa tgaagcaacc agaaaagtca gaagaaagtc cacagccggt
     4441 ttttgcaacc agacccgctc agcaaaacga taaggatgcc agtccatccg cgccatcccc
     4501 tgaaatctcc tggcagcgga catggccgat atttatggcc ggaatggttg tgatggcctg
     4561 tctcggcgga acaggattat ggggctggtc gcagcttaat cagccggacg cactaatcca
     4621 gcgaatacaa ctgtctgtca tgccattacc gcagtcgctg gagagcggcg agctggcaaa
     4681 gctggatgta aaggataagg cgctgctggc tcaggacaga acaattgcgg caagtcagat
     4741 gcaactggag cagttaaaca aattgcctgc ccgctggcca ctggagcagg gatatcgcca
     4801 gctacgccag cttgatgccc tgtggccgga taatcctcag gtcagagcgc tgaacgcgca
     4861 gtggcgtaaa cagcgggagc tgagcgccct gtccacagag gcattgaatg gctacgcaca
     4921 ggcgcagagc cagctacagc gcctgtcggc gcagctggat gcactggatg agcgtaaggg
     4981 gagatatctg accggttcgg aactaaaaac ggcggtgtac ggcatccggc agtcgttaaa
     5041 ggagccgccg ctggaagaac tgcttcggca actggaagag caaaaacaga ccggagaggt
     5101 ttcgccgacg ctgttgacgc aaattgatac ccggttaaat cagttgctga atcgctatgt
     5161 aattttactg gatacgaagg tggaacaaag tcagtaaacg tcgggggatt tatgcaccaa
     5221 taagggatat gtgaccttgc cattttttaa aaggagtctt tgtgtacaaa aaagagagga
     5281 acaggccatt cgaaacgcgt gaagaggttc tggcatatca taagcgggaa aaaataccct
     5341 gtctggagtg tggcaagttg ctcatttttt taccgcgcca tatctggttt gtgcatggaa
     5401 tacgcgctga cgaatatcgt gtaaaatgga acataccgaa gcacatcgca ctggcaggga
     5461 tttcatatct ggagaaacaa agtgaatata tgaaggcgag aatagcaaag ggaaagctgg
     5521 atcccgcaga acaaatagcc atgatgagaa taaagggaag gcaactaaag tcagatcctg
     5581 actggcgtaa tcgctccggt tctatattga agaaacaata tgcccgacga gatatcctta
     5641 aaaacagggt atgggaaaga tctcccagca taaaaagtgt ctctgtggag ttgaaaacag
     5701 aagctataag gcgaatgaaa aacaggaagg tcgtcgggga aaaagtccag gatattgccg
     5761 atgacctgaa cgtatctatc agcagattgt atcactggct aaagatgtag gcgctcatgt
     5821 gctctcaatg ccttcaataa agccgctatg cgcgaaccgg cacatctggt agactgttct
     5881 cagtgagtca agcaaactgc ctacttttct aacttttaat catgaataac cagcgccttg
     5941 agtatagctg gttcgaatat actcgttgat tcacatcaga taatgagtgt ataattcggt
     6001 agatagcttg ggttcgggaa cagtgaatgc cttaagtttc aaaaggttag attaacctct
     6061 tgactgtggc ccccgcaacc aattattgaa caccctaacg ggtgtttttt tgtttctgct
     6121 ctcccataaa aaaagcgcca ttcagcgcct ttttatcatc cccttctcgc cagcgtcgcc
     6181 ccatcggcaa aatacgcttt aattcccgca agaatagact ccgcaacttc ctgctggaaa
     6241 gtcgccgttt tcagtttacg ctcttcctca acgttactga taaacgccgt ttcgaccaga
     6301 atggagggaa tatctggtgc ctttagtacg gcaaacccgg cctgttcaac ttgattttta
     6361 tgcagcttgt tgattttacc gagcttattc agtaccgctt taccaaactt caggctgtcg
     6421 gcaatggtca gcgactgcac catatcgaac atggtgtggt cgacatagcg gtcaccgctt
     6481 ttgctcacgc caccaatcaa gtccgaggcg ttctgggttt gtgccagata ttttgccgca
     6541 gtactggttg cacctttggt tgagagcgca aacacagagg aaccgctcgg ctgacgactg
     6601 gtaaaggcgt cggcatggat agagacaaac aggtcagcac gctgtttctg ggcttttgct
     6661 acgcgcactt gcaacggaat gaagatgtct tcattgcgcg tcatgtacac cttcatattg
     6721 ccctctttct cgatcagaga gcgcaaacgg cgagctattt gcaataccac gtctttttcg
     6781 cgcgttttgt atttccccac cgcaccggag tcttcgccac cgtggccagg gtcaagcata
     6841 atgacaatcg gacgatcgcg ccctgcttta cctggttgtg gaccactttg cgctggcggc
     6901 acctgctttt cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag cgggtcctgc
     6961 atatcctgtg cattggccgg atagaggtcc atcaccagac gctctttaaa cccggcgacc
     7021 ggcgcaaggg caaacagctg cggttttacg ttttgcttta attcaaaaac catccgtacg
     7081 gtttgcgggt caaattgccc gacgcgcgcc gacttgatga acgggtcgtc tgcacgaatt
     7141 tgtgccgcca tccccttgag taccgagttc aggtttacat cttcgatatc caccaccacg
     7201 cgttccgggt tactcaacgc gaactgctta tatttcagct gacgatttga ttctaccgtc
     7261 acacgggtgt agctggacgc aggccagacg cgcaccgcca cgacctggct gaccgcagcc
     7321 agactgacct gacttacgct caatagccac atggcacccg cgccttgcag taaacgacga
     7381 cggctgattg cagtgttgga tcctgacatg cctctcccga gcaaaagaaa tctaagctgt
     7441 gtaacaagta aacgactaat ttgaccggaa actttagcga aagacgcata atctgtcatc
     7501 taataaacgg taaacattct ttttatattc acggcattac tgataaaaaa gtcgctctcg
     7561 cataaaattt acacttgcac cctgcgaaaa aacagaataa aaatacacta atttcgaata
     7621 atcatgcaaa gaggtgtgcc gtggtaaagg aacgtaaaac cgagttggtc gagggattcc
     7681 gccattcggt tccctatatc aatacccacc ggggaaaaac gtttgtcatc atgctcggcg
     7741 gtgaagccat tgagcatgag aatttctcca gtatcgttaa tgatatcggg ttattgcaca
     7801 gcctcggcat ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac gcaaatctgg
     7861 ctgcgcatca ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac gccaaaacac
     7921 tggaactggt gaagcaggct gcgggaacat tgcaactgga tattactgct cgcctgtcga
     7981 tgagtctcaa taacacgccg ctgcagggcg cgcatatcaa cgtcgtcagt ggcaatttta
     8041 ttattgccca gccgctgggc gtcgatgacg gcgtggatta ctgccatagc gggcgtatcc
     8101 ggcggattga tgaagacgcg atccatcgtc aactggacag cggtgcaata gtgctaatgg
     8161 ggccggtcgc tgtttcagtc actggcgaga gctttaatct gacctcggaa gagattgcca
     8221 ctcaactagc catcaaactg aaagctgaaa agatgattgg tttttgctct tcccagggcg
     8281 ttactaatga cgacggtgat attgtctccg aacttttccc taacgaagcg caagcacggg
     8341 tagaagccca ggaagagaaa ggcgattaca actccggtac ggtgcgcttt ttgcgtggcg
     8401 cagtgaaagc ctgccgcagc ggcgtgcgtc gctgtcattt aatcagttat caggaagatg
     8461 gcgcgctgtt gcaagagttg ttctcacgcg acggtatcgg tacgcagatt gtgatggaaa
     8521 gcgctgagca gattcgtcgc gcaacaatca acgatattgg cggtattctg gagttgattc
     8581 gcccgctgga gcagcaaggt attctggtgc gccgttctcg cgagcagctg gagatggaaa
     8641 tcgacaaatt caccattatt cagcgcgata acacgactat tgcctgcgcc gcgctctatc
     8701 cgttcccgga agagaagatt ggggaaatgg cctgtgtggc agttcacccg gattaccgca
     8761 gctcatcacg gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag cagagcggct
     8821 taagcaaatt gtttgtgctg accacgcgca gtattcactg gttccaggaa cgtggattta
     8881 ccccggtgga tattgattta ctgcccgaga gcaaaaagca gttgtacaac taccagcgta
     8941 aatccaaagt gttgatggcg gatttagggt aagcgatgaa aattgtcgga tgcgacatgc
     9001 gtaacactcg tgcgtcgcat ccggcaatta cgtttattcc cgcgaactaa acaacgccgc
     9061 cagaccactg cgccgctcag tacgagtggc gattgccgca cttaatatac gctcatcggc
     9121 atacagcgac agacggcgac gcgcgcgggt caccgcggtg tataccagct ctcgcgttac
     9181 taccggcgtg cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg atccctgcga
     9241 tttatgtacc gtcatcgccc acgtagtttc gtgctctggc aagcgactcg gttgcacaga
     9301 cttaatattg ccgtccggca tcgcaaacca gacgcgcgtc ccctgcccgc gatccagcgc
     9361 aataccgata tcgccattaa acaacccaag cgcgctgtca ttacgggcaa tcatcaccgg
     9421 tcgaccttcg taccaacgag agtgcggatg acgatgaatt ttgcgcttct gttgcataaa
     9481 ctgctcaatt cgctcattca gtccagccac gccaaacggc ccttcccgca gggcgcacaa
     9541 aagttggtac tcattgaacg cctgaatgat taaatccggc tcagcacgcg cttgcagcag
     9601 atccagataa cgtccataac ccgcaagagc ttcctcaagc atcgcaatat aatcttcgcc
     9661 gctctgtaaa agtcgttttt cgatatcagt aaaatcctgc tgaaaaacgg ttttcactgc
     9721 cgttttatca ccacggttga tcgccgcagc taactgacca atgccagaat cgctgccgaa
     9781 acgatagctt ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt ctgtgccggt
     9841 tcctgccgga acgtgagtcc ccgtcaggcg gcttagctgc ccggcacgct cggcggtaaa
     9901 gccggcattg gcataagcgc agatatcgcc cagcacagcc ccagcctcaa ccgaggccag
     9961 ttgatcacga tcgccgagaa agatcactcg cgcatgatcg ggcaaggcgt cgatcagtct
    10021 cgacatcata ggcagatcga tcattgacgc ttcatctacc accagcacat caagatgcag
    10081 cgggttaccg gcatgatgac gtaaacgctg gctacccggc tgcgcaccca gcaatcggtg
    10141 caaagtactg gcatcttccg gaatgcgttt cttttgttca tcggtcagcg gtaactgtcg
    10201 caaagccttg ccgagagatt cggttaagcg cgcggcagct ttacccgttg gtgcagccag
    10261 acggatacgg cagcgttcgc cgtcggccat ttgaattaac gctgccagta acttcgccac
    10321 ggtggtcgtt ttaccggtgc ctgggccgcc ggaaatcacc gagatccgcc gcgtcagcgc
    10381 cactgccgct gcaacttttt gccagttaat ttcatcgctt actggaaaaa gtttgtccag
    10441 ggtttgcgcc agtagagctt catcaacctc aatggcatga ttcacctcat tgaagaagcg
    10501 cgctaccgtc cgctcgttac accacatgcg attcaaataa agacgatcgc cacagaggat
    10561 catcggcgtg ggttcatctc ccctgctgac cgcttgagaa gccagcaagc attcttccca
    10621 attttgtagc tcaccgattt cactgacaca ggtcgccaac agcggatgcg acgcctcgtt
    10681 attttccagt cgtgaaagcg gcaaacagac gtgtccctct ccggcatcat gacttaacag
    10741 tgccgccgcg agggtgacgg caggatgttc atctcccgcc acggtcaggg caaattgcac
    10801 atccagcggg cgtagctgtt tgtgctccac agcttccagt aattgctttt gcaatttcat
    10861 tacgcctcct ccagggtcat accggcaaac atctcatcca tcaggtcaat caacccggcg
    10921 ttgggtcggg ttgtgtaaat cccttgttgc ggatgttctt tatcaacacc acgcaggaac
    10981 agataaataa cgccgccaaa gtggcgctca tagtcgtaat cagcaatgcg atggcgcaga
    11041 taacgatgca gcgccagggt ataaagctga tattgcagat catagcggtg tgcctgcatc
    11101 gccgctgcca tagcctgttg ggtgtaagcc gaactgtctt cacccagcca gttggattta
    11161 tagtcgagca ggtaataacg cccttcgtgg cggaatacca ggtcgataaa gccttttaac
    11221 atgccgcgta cctgcatgaa ctccagcggc gggcagccag cggatagcgg atcaaactgg
    11281 cgaattagtg catcaagctg gctggcgatg agcggttcac taatcggcag gtaaaactcc
    11341 atctccacct gtttatcgcg gtcggaaagc tgattcaggc taacacccgt ttcattgaga
    11401 ggtgcctgga ggacagccgt gatccactcg gtcaacactg gttcccactg cgattcaaag
    11461 ccgccgagtt ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt aaaatcgagg
    11521 tcttcaaaca aactgtgcaa gaatgtccct ggtgacgcac cgcgcgggaa ctgatgcggt
    11581 gttaacgtcg gttcttcaac gacgctgacc acgcctgcgg catcaacatc cagccgtggc
    11641 atcagatcct gagcgatacc gtgaccacgc tgctgcaaac cggagtagct ggtgacacgc
    11701 cagttatcgc cgggcaatcg ttgtagcgtc ctcgcattca gttctgcagt taaagcatca
    11761 ttaacctgcc agggctgttt atcacccgtt tgtgccgttt gccaggcaat atcatcatcg
    11821 cataacgctt caatacaggt gcgaagccct gccgcatctt gcggttcccc tttttgcagc
    11881 aaacgcccga gcgcactttg gtggacgtcg gtgtcacctt ttttatcgcc acgacggcgc
    11941 accagcggtg caacgccgag actgcaatgc caaaccgaac gtgtcagcgc cacgtaaagc
    12001 aaacgcagat cttccgccag acgttcggcc tccgcgaggt cgacgctttc tggcgcagca
    12061 ttaagatcca gaactgcctc aaacgagtgg cgatcgtgat aaaacgcctg atcctggacg
    12121 cggaaattgg tgataaacgg cagccagacc aatggatatt ccagcccttt cgatttgtgg
    12181 atcgtgacaa tctgcaccag atgtttatca ctttcgagac gcatttgttg gctggaggca
    12241 ttactgtctg gctcgaggat atgttgcgat aaccagcgta ccagcgcatg ttcactttcc
    12301 agctgcgttc cggcttcttg tagcagttcg ctgatatgca agatatcggt aagtcgccgc
    12361 tcaccgcctg ccgttgccag caagttttct gcaatgttac gcgccgacat cagcgcccgc
    12421 agcatcggca taacgccacg tttgcgccag atttgccgat aaccatcgaa ctcttcgacc
    12481 accgcgtccc acgcatgttc gtcattattc agcgtttcaa tatccagcgc attcagcccc
    12541 atcattgacg ttgccagcgc actgcgcagg gtgttctcac gttcgggcgt catcaccgcc
    12601 tgcaacaacc aaagcatttc ctgcgcttcc agagtttcaa aaacactgtc gcggttcgaa
    12661 aggtaaacgg aagggatttc cagcaacgtt aaggcatcgc gcacctgggc ggcctcctgg
    12721 cggctgcgca ccagcacact gatgtccgaa gcacgcaccg gacgcgcgtc gtcgccgttc
    12781 atcagcaacg cttcgccccg ctgtccggct tgtagccagt cgcggatttg cgcagcacat
    12841 acctgcgcca tggtactttg ataatcgcca acgccgcagc tttcgccttc catcagccac
    12901 attttcatcg caggctgcgt ttcaccttta aatacaaaac gtaacgcctg atttttcccg
    12961 actgatttca ctggaataaa cggtatttcg cgaaacatga acgcgtcatc agtctggctg
    13021 aaaagcttat tcacgctgtt caccattcct ggtgcggaac gccagttggt gtctaaagtg
    13081 tagtgggcgt gaacttcgct acgcgccttc atataagtga agatatccgc accccggaat
    13141 gcatatatgg cctgcttcgg gtcgccaatc agcaacaatg cggtttccgg ctgatggtgc
    13201 cagatacggc gaaaaattcg gtactgctgg gggtcggtat cctgaaattc atcgatcatt
    13261 gccaccggga atcgcgtacg gatcgccgct gccaacacct caccgctttc gctacgcagc
    13321 gcggaatcga gccgacttaa catgtcatca aaacccaatt cgccacggcg gcgtttttca
    13381 cgcgctactg tttcgcggat ctcagccaat gcgcgggtga tcaccagatc gcggatcgac
    13441 aatggttctg caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg ggtttccccc
    13501 ccggctttcg tgcgatcttc taagaaacgc tgggagaatt tttccagcga ctccggcaac
    13561 tgataactgt tggtctcttc ttctgcccag gcgctgatct tgtcgatcca tttagcctga
    13621 ttgctacggt taaacttgcg tcgatcaata ccagaagatt cgatcagcgc atccagttca
    13681 cccactgcgt cgcgccactg ctgttttacc gtagcaatac gcgccacaat ttgcgcgtgg
    13741 cgggaagcca gcgtttcatc atcgggcggc ggtgctttga taaccggcgc ttcgccttgc
    13801 agataacgat taatatcgcg cagcaacgcc tgcggccctt tccaggtttc aaagacgacc
    13861 tgggctattt cacgcggcag cgggtagcag tggcgacgcc agaaatcggc gcaggcctgg
    13921 taacgtagca gagactcatc ttcaatcagc tgctgctcaa acagcatgcc ggattcaaag
    13981 gcattcaggt tgagcatgcg ctggcaaaaa ccgtgaatag taaagactgc cgcttcatcc
    14041 atctgccgtt cggctaacaa caaccactgc gcggcttgcg ctttatcgtc gatctcttcc
    14101 agcagacgtt cgtacagtgg attgtcggtg gtttcacgca gacaggcgat gcgcaactca
    14161 tggatattgc tacggatacg accacgcaat tctgccgtgg cagcctcggt aaaggtgacc
    14221 accagcagtt cttcaacggt cagcgggcgg ggaaaggcgg cggaaccgcc tagtccaagt
    14281 aacaggcgca aatagagcgc cgcaatcgta aaggttttgc ctgtgccggc agaggcttca
    14341 atcaggcgct caccctgtaa gggcaagcgc aaaggatcta gtgtctcggc gacatcactc
    14401 attcttttca ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct cccacacttt
    14461 ccagccttca gggtgtacat attcggcttt cccattctgg ctgccggaaa tctgcgacag
    14521 gatagccatg ccttgcggct cgactaccgc ctgatggaag aaatcggcca gtttttgcgg
    14581 cgtcagcagt ttaatctggg ccacgatttt atcacgcgaa tcgaagcgca tattgccgcg
    14641 atcgaaatct ttacttaact tcgatgcttc ttcgccgagc gtttgcggtg cctgcagcat
    14701 ctgggtaatt accgcctgct ggatttgcgc aaactcatct ggcttcatcg ctcgcaattt
    14761 tgcctctgca gttgggaaaa acgccttgta acgctcccac aagaatgaag gctgtttatc
    14821 attgctttgc aaaaggaagc ccatgcccca ctgacgcccc acgctcattg gaaacgcaaa
    14881 cacggcatag cccaattgtt cttcggtacg caactgattg tagaaccacg gctgtacgat
    14941 ctgccccaac agagagctat aggctgagct ggtgtattca tcgtagccag tcggtacaaa
    15001 taccgctgcc agtgcggagt cggtgctgtt accggctttt tcaaagatga cggattgttt
    15061 tttatcgacc actacatctt tgtttcgaca ccactctgaa ccatcagcgc ccaactgttt
    15121 ttgcacatcg cgtgccagcg ttgttgcctg ggcttcggtc atgttgccaa taaccataaa
    15181 ctctggtcga gcccctgatt ttaaggcgtc gcgataggcc agcacctctt tcaacgtaat
    15241 ggagggcaaa attttgcgcc gttcatctcg cgagaagtac ggcacttgcg agagcatctg
    15301 cgcgggcata atcgcctgct cgaacgcttt gcccttttct gcggaatcca tcatctggtt
    15361 ataccaggac ttcgcctgct caagctgatc ttccgtggcg gtatagctaa agtagccttc
    15421 cagcaatgcc tggaacagct gcggcagacg ctgggtgtaa ccattagcat taaccataag
    15481 gccgttgtta gcgttggtgg aaaaacttat gccaccaacc gacgcctggt tgcttaactg
    15541 atcaagcgcc agccctgcga gataatcatt gagcgcaaac atcacctgat tgcgggcgct
    15601 gtccatggct ttcggattac gcaaaatcag gctgacatcg gcttttggct cgctggcaaa
    15661 ataacggctt ggcgcataca ccacgcgcag attcgactca tcaacaatca gctctggatg
    15721 gtcatatttc ttgtctgact taatcagcga gaaatcgtca ggaatataag ggttaagctc
    15781 tggcaaagag agcgcaatgt cggcggcttt tttctgccag tcggcgaaag tttgtgcgct
    15841 gattttatcg acctgatacg gcgcatcgac aaagtaagcc gttttgttgt gcggctcttt
    15901 cgggctgata taccagatac gcgcattctg cggcgtcatc atcgccagac gttcctttac
    15961 tgctttagca tcgtaccgat cggcaatatt gactgcatcc agcgtatgct caacaggaac
    16021 gcgaatcatg gtatctgcca gccattcgac gtaatccata tcacgggtga ttgacggata
    16081 acggaagtcg atatccagca cattcgccag ttcatcgaag tattgtttat cgatcccttt
    16141 ttcacgtaac aaattgaggt agctaaaaat agccgccaca acctgatcgc gattcgccag
    16201 acctttatcg gttaaagacg cagagatcgc taatacgccg ctgttgccgt tgacgatagg
    16261 atcggagttg gcgctaatgc cctcaactaa tccctgcttt tgcagccagt cggaaagtgt
    16321 acctgggctg cgattaccaa tcagataggt aatcaattca tcggttttgc tacggaattt
    16381 cgccgagttg ttatcaatac gaaactctac gcgcaacact ttacgcggca gcgccgggac
    16441 gtaatgaatg ataatgccct tttgcgcgtc ggtgactacc ggcacggtga tttccggttt
    16501 tttgctctct ttgttcggca cgcgaccaaa ggtgtccgcc gccatttttg ccaactccgg
    16561 cagcggttta ttgctgtaaa taaccgcctt catcagattg gcggaatagt acttctcgtg
    16621 gaaatctttc agcgcctgct gcaccggatt accaggtttg tcgcttaaag tttcgaggtt
    16681 accaccagaa aactttgaac cggggtgtgc cgggttaatg gtttctgcgc tgacctgtgc
    16741 catgcgcatc ccgtcacgcg tacgcgccat ggttaattca gcgttcactg cattacgctc
    16801 acgttcggcg tatttcttgt cgagcaaggg ttctgcaata gcatcagcca ggcggtctac
    16861 cgcaccaggc aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat acggcgcagt
    16921 gctggcattg tgactaccgc cgtgcatttt gagatattcg gccagactgt cagcctgcgg
    16981 gtactttttc gaccccatca gactcatatg ttcaaggtaa tgtgccagcc cctggtacgc
    17041 ctcgggatct tccagcgacc caacgggcac caccagcgcc gagagcgatt taactgcctg
    17101 cggatcagaa accagcaaga ccaccatacc gttatccaga cgtatagcct gatactggcg
    17161 gttatcttta tcacttttac ggatggtttc ctgaatcggc tgccatcccg tttctgcctg
    17221 acttaagggt gcccaaaggg caactaacaa caataatgct ttgaaccagg tgctgcgggg
    17281 cattcacgga cctcataatc aacttaattt tctgtccaga ttcaacacgt taacgcaaat
    17341 catctgccag aatttaatct tgtgctgcac gagtcagcct atgtttatat aaccatcagt
    17401 ccgtgactgg tgcgcatcat aaagcaagcg gatagattgc gcaattttta tacagcactc
    17461 atgactgatt aaagcgaaac agcggtaaca ggaaacgttg cgactgttca acgatggcct
    17521 ccattgtctc tggtgttaat tgccgccaga gccgttgata ccagatatca tcaccttcgc
    17581 cacgcaccat catgttgcct tcgtaagcct gcaggaattt cgtacgggct ttttgcaacg
    17641 tggaatcgtc atccagcatg gcatcgtttt gcgcgtcata acaggttttt agccacgcgc
    17701 cgccactttc aggtaacacc agcaatggcg cggacatccc ttcacgatac ccctcaatca
    17761 gttgtgagag gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc cactcgccgt
    17821 ctttgcgtag aaaaaggcga ctttcaccat taccaccgct ggcacagtag acaaggtgtt
    17881 ccagccaaag ttgcattccc tgcgccacac ttaataaaga gggacgccag cgcaacaagc
    17941 catccggctg cacctgcggc aaccagccag ttatctgcac accgttgcag gcgagatcaa
    18001 tttccatact ctgccccggc tggcgacagg caatgactct gtcggcaagc tgctgcatct
    18061 catggcactg tgtttcccag aaaatttcac caaaagcgcc atacggtaaa tcccctgccg
    18121 cccggaagcg gcggaacaag cgttcggcat catcctgctc aaccagtgca ttcaataact
    18181 gctgattgat ttgataacga ctaagccctt caagaataaa tggctcggtg tcggggattt
    18241 cgctgtcttc agtacggaag ttcacctgca aacgcatctg gaagaacgcc cgcaccggat
    18301 gtgcccagaa tcgttgtagc gtttccagcg gcacggtttc cggtaaggta aacggcagcg
    18361 gctgaacaaa ttcagaatgt gctttaccag cctggctggc cgcaggtagc cattcacgag
    18421 catagctttg tcgttcgcct ggctggtagt tttgtggatc aaacggcatc cgggtatgga
    18481 ggcaagtaag atgcgctttt acccttgcct cgctttcatc acagttgagc gcttcatcgc
    18541 ccggtagata atgactttgc ccgatgtagt cgatcagttc ctgcaccagt accgacggga
    18601 aacgttcact gttatcctga atggagcgcc cgatatagct gatataaagt ttttgttgcg
    18661 cggaaattaa cgcttccagg aacagatagc ggtcatcatc gcgacggcta cggtcgccac
    18721 gcttcggttt ctggctcatc aggtcaaagc ccaatggcgc aagctgacgt ggataaacgc
    18781 cgtcgttcat tcccagcagg caaaccactt tgaacggaat tgaacgcatt ggcatcagag
    18841 tacaaatgtt aaccggtccg gcgagaaaac gctggctgat acgttcttga tccagacgct
    18901 gcgccagttc atcacgcaat agtgacagcg gcaccgcgtc gccatactgc gcacctaaac
    18961 cttcggcgat aatcgcctgc cattgttgtt cgatcagcgt catcgccgct tcggtttccg
    19021 catccggcag gaagaaggcg ttgagcatat cgcgacaaac cggcaaccac tcttctagcg
    19081 gacgctcctg tgccagcccg cgacgccaga tgtttagctg cattagcagt gaagccagat
    19141 gccccaccag ttctgcaatt aagccgctcg attcatcata aggtagaacc gattgccact
    19201 cgccctgcgc gctctccatc gcgtagccca gcaacatacg cgtcaggcca aatcgccagg
    19261 tgtgttgtcc ggtggcgggg agttccagct cgcgaacgtt gtcgtcatct atcccccaac
    19321 gaatgccgga ttcgttgacc cactggcgta aataacgcag cccttcttcg gtgatgtcaa
    19381 accgcgccgc cagcaccggc acatccagta atgccagcac gtcttccgac acaaagcggc
    19441 tgtctggcaa cgataacagg ctgataaacg cttccagtac cggatgcgac tgccgcgccc
    19501 gacggtcgga aatggcgtaa ggcaggtaac gatccgcagg tgcgctacca aacacagcct
    19561 gaataaacgg actgtagctg tcgatatcag ccaccatcac gatgatgtcg cgcggagtaa
    19621 gtgtcgggtc ttcttccagc atcgccagca ggcgatcgtg caaaacttca acttcacgct
    19681 gcgggctatg gcaaacgtgg aaggtgatac tgctatccag tggatcaagc gggcgtttgt
    19741 tatcgctacg ggaaaactct tcgatgttca caccagcaac ggcacggttt tccagttcca
    19801 gaatgtcaga ctgaatgtta tgcagcaggt tatctggcgt gacatcgaca aaggcgtcca
    19861 gctcctggct gctctccagg tcagaaagga gataaatgta gtcgcgccca agcttacccc
    19921 atgaagccag cagcgggttg ccgacatcct gttcaccatc gctgttaaag agctgcccgg
    19981 cattttcgct atcacgaaat agcggcaatt cgcgatcttc aaaactgtgt cgacgctgac
    20041 gggtcagcag tttcgccaga taagcaggat ctttaatatc gccccagtaa taacggcagg
    20101 ggttggtaaa caggagatgg atttcaatat gtttacccag cgcctgtagc gcctggagat
    20161 aaacaggcgg taacgcggaa ataccgcata taaagacgcg cgaaggtagc cccggcgggc
    20221 aggtcgtcgc ggactccagc gtttcgataa agcgctgata gagattggcg cggtgccagc
    20281 gcggttgccc gagttgatgg gtatattcca ccaacgcctt ccacaacggc gcttgccagg
    20341 cctgtgcttc tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc agccagtccg
    20401 gacgatagac cagatactgg tcaaacaggt ccgccgcttt tgaagaaagc tggaacagtt
    20461 ttcgcttgtc gctatcgtca gtcagataat gccgcaacag ggtaaagtct tcgcgctcca
    20521 acaattgcgg cagcagagtc atcagtttcc agctcatgct ctgtttgtta aaggcgctct
    20581 ctttggggat ttccggtaac acccggacga acatatccca gataaagctc gctggcagcg
    20641 gaaaatcaat gtttgccgca ataccaaact tttgcgacag ggtcatttgc agccactgcg
    20701 ccataccggt actttgcacc agaatcatct ctggttcgaa aggatcgtcc agccgttcgc
    20761 gttcgacaat aaactccatc aacgcttcca gcacgtccag acgattggaa tggtagaccc
    20821 ttaacatagc ggctcctgac tactgacgat tcgggcaatg caggcgggtc atctcgcctt
    20881 ctctgccccc gggtgaaact agcgtaacgc tgatgctgac acatcccgcc tgcgatgtct
    20941 gcattcggtt gacctgccag ttggcaggtg gcgaaatcgc gcgcagttgc gtttgctgcc
    21001 aaccatgccg ccagagctgt tggtactggt ttctgctggc gagactgttc attaatgttc
    21061 gctggatacc cgataacgca gtgacaatca tcaccatcag caccatcgca accattacct
    21121 ccggcaggct aaagccttgt tgattcttca gggaagctga cataacgccc tctctttcag
    21181 cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatg tttccattaa tgacttcgcc
    21241 tgttcgccac aacgaaacgc cttcataacc ggcaatcaat aaggcttcat tatctgccag
    21301 taaacgcaaa caaacctggg catcggttcc agcgtactgc gagcactgaa ctgctggctg
    21361 cgtctgccag gagtgcattt ttccccacgc cagcgccgac tgaacgatgg cctggcggcg
    21421 caatgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct gactcattcc
    21481 ttgcaatagc aagctaccca aaaccagcag catcaggacc agagccagtg acgaaacacc
    21541 tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac cacggtttgc
    21601 ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa gccgctgaca
    21661 tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt gactttatcc
    21721 cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg ctccttcaga
    21781 cgaaatccaa tctggtcaga ctcttttacc ggttcgcgat cccagatacc gttactgttc
    21841 gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc ttcgccggta
    21901 cagctgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa tacccgcagc
    21961 cagatttcat cttccagcgc cagcttacgg gtgctcgtta aactttcacg ctgtaacgca
    22021 ggcagaaagc gtgcagcccc cagcaacaat acgctactga tcgccatagc aatcaacact
    22081 tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct tgctgacaaa
    22141 gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag tttttgaagc
    22201 gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa ggtgtcaggt
    22261 cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg ccatgacagg
    22321 tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg ataacgctga
    22381 tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa tagtcccgcg
    22441 cctggctggc ggtttgccat agccgttgcg actgctgcca gtattgccag ccatagagtc
    22501 cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc agcgtataac
    22561 cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga gggccagttt
    22621 ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg caaaatttcg
    22681 ggaaggcgtc tcgaagaatt taacggaggg taaaaaaaac cgacgcacac tggcgtcggc
    22741 tctggcagga tgtttcgtaa ttagatagcc accggcgctt taatgcccgg atgtggatcg
    22801 tagccttcaa tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg tttacgtttg
    22861 ataatcaact tcggcagcgg acgcggttcg cggcttaatt gcagatgagt ttgatccata
    22921 tgattgctgt acaggtgcgt gtcgcctccc gtccagacaa aatcgcccac ttccagatca
    22981 cactgctgcg ccatcatatg caccagtaac gcgtagctgg caatgttaaa cggcaggccg
    23041 aggaaaacgt cacaggagcg ctgataaagc tggcaagaga gtttgccgtc tgccacatag
    23101 aactggaaga acgcatggca cggtgccagc gccattttat ccagttcgcc tacgttccac
    23161 gctgaaacaa taatgcggcg cgaatccggg tcgtttttca gctggttcag taccgtagtg
    23221 atctggtcaa tatgacgacc atccggcgtt ggccaggcgc gccactgttt accatacact
    23281 ggcccgaggt cgccgttttc atcggcccat tcgtcccaga tggtgacatt gttttcgtgt
    23341 agataagcaa tgttagtgtc gccctgcaga aaccacagca gttcatggat gatggaacgc
    23401 aggtggcaac gtttagttgt caccagcggg aatccatcct gcaggttaaa acgcatctga
    23461 tgaccaaaaa tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt gccttcgtcg
    23521 agcacttttt gcatcagttc taaatactgt ttcatggttc ctcaggaaac gtgttgctgt
    23581 gggctgcgac gatatgccca gaccatcatg atcacacccg cgacaatcat cgggatggaa
    23641 agaatttgcc ccatgctgat gtactgcacc caggcaccgg taaactgcgc gtcgggctgg
    23701 cggaaaaact caacaatgat gcgaaacgcg ccgtagccaa tcaggaacaa gccagagaca
    23761 gctcccattg ggcgcggttt acgaatatac aggttgagga taataaacag caccacacct
    23821 tccagcagca gctcgtaaag ctgtgatggg tggcgcggca gcacaccgta agtgtcgaaa
    23881 atggattgcc actgcgggtt ggtttgcagc agcaaaatat cttctgtacg ggagcctggg
    23941 aacagcatgg caaacgggaa gttcgggtca acgcggcccc acaattcacc gttaataaag
    24001 ttgcccagac gcccggcacc aagaccaaac ggaatgagtg gtgcgataaa atcagaaacc
    24061 tggaagaagg aacgtttagt acggcgggca aagataatca tcaccacgat aacgccaatc
    24121 aggccgccgt ggaaagacat gccgccgtcc cagacacgga acagatacag cggatcggcc
    24181 ataaactgcg ggaaattgta gaacagaaca taaccaatac gtcccccgag gaagacgccg
    24241 aggaagcccg catagagtaa gttttcaact tcatttttgg tccagccgct gcccggacga
    24301 ttcgcccgtc gtgttgccag ccacattgca aaaatgaaac ccaccagata catcaggccg
    24361 taccagtgaa gcgccacggg tcctattgag aaaatgaccg gatcaaactc cggaaaatgc
    24421 agatagctac tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa gagaacagcg
    24481 attgaaatgc gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag cacaagggga
    24541 cctggctggt tgccggatac cgttaaaaga tatgtatatg atccgcgcta taaccctccg
    24601 cgaatcagcc cgcccatgcc gcgacgctcc ataaaggctg caacctgatg gcgaacttcg
    24661 gtcgccagtt gcgcttccag actacgctgc gcaagatttt ctgcttcggc ataatcaatg
    24721 cgccgcagca ggtattttac ccgcgctaca gaacgtccgt tcatagacag atggcgatac
    24781 ccaagcccaa tgaggattgc cacgcacatg ggatcgcccg ccatttcacc gcacaaacgg
    24841 agatcgattc catgtatttc cgcttcccgg gcgatcatcg ccagagctcg taacattgca
    24901 ggatgaagac tgtcataaat gttcgccacc cgggtattgt tgcgatcaac ggccagaatg
    24961 tattgagtca gatcgttggt gccaacagag atgaaatcga cccgctttgc cagatgcggc
    25021 agcataaata ccattgacgg cacttccagc atgatgccga tacgtggttt gggaatttcg
    25081 taaccgatca tctcctcgac ttcacgtccg gcacgttcaa tcaggcggcg tgcttcatca
    25141 acttcatcga ggcttgtgac catcggcaac agaatattca ggttgcccgt agcggcatta
    25201 gcacgcagca tcgcccgcac ctggatcaag aagatctccg gctgatcgag cgtaatgcga
    25261 atcccacgcc aacccaggca tggattctct tcgctgatcg gcatgtaagg cagctgctta
    25321 tctgctccga catccagcgt acgcaaggtg acgggtttat cattgaacat ttgcagcatc
    25381 ccctgatact gcgccacctg ttcttcttcc gacgggaaac cactttgcag catgaatggg
    25441 atttcagtgc gataaagacc gatgccatca atacggctgc ccagtttttc ttcatgttcc
    25501 gggcttaaac cagcattgag catgactttt atacgctcac cgctttttaa ctgcgcgggt
    25561 aaattgacgt catcttccgc cagacggcta agctcaatct cttcactaat tagccgctga
    25621 tattcttgca gcagtaccgg ctccggatcg accagcaatt caccgcgata gccatcaacg
    25681 atcagcgtcc tacgatgcag caccgaaggc tgaatatccg cgcccatcac ggtagggatc
    25741 cccagcgcac gtaccatgat cgcagcatgg gagttggctg cgccatctcg cacgacaaca
    25801 ccgactaagc gatcctgggg cagctcagca agagttgtcg ctgacagttc atccgccacc
    25861 agaatgaaac gttccggcca ggcgttcggc ccttgattag cgtcatcaag atgaaacagc
    25921 aatcgctgac ccagcgcacg taaatcgcca gcccgctctt tgagatagtt atcgcttagc
    25981 gcggcaaact gttcggcaaa tttttcaatg accgttttta ccgcccactc tgccaccgag
    26041 cctttatcaa cctcggcaaa caattcgcga cgcagccggg tgtccgaaag caggtgcgag
    26101 taaagatcga aaatagccgc cgtttctttt tgtgcaccgg cggcaaagcg tttgctgtag
    26161 cggcgaaact cgtttgccgc ttcttccagc gccccggtca gtcgttcgcg ttccagagcc
    26221 ggatccagcg ttgatgcctg atacacctgt tccattaaag gtaacgtggc atcctgccag
    26281 ccttcggcaa tcgccacacc aggtgctgcc ggtaatgcgc ggattcgcgt ctggcgatat
    26341 tgcccaaaca gggcagtcaa ctgcgactga gaaagaatag ctgccatctg ggtggcaagc
    26401 gtcaccagga aggattcttc actttcgtca tactggcgca actctcgttg ctgtaccacc
    26461 agtacaccaa gcaactggcg acgttgaata attggtacgc ctaaaaacgc gcggaaacgt
    26521 tcttctttta cggaggggat gtatttgaag ctggggtgct tttgcgcatc tgcaaggttt
    26581 atcggttccg ccagcctgcc aaccaggccg acgatccctt catcaaacgc gagcgttaca
    26641 gtgcgaccgc gtggtttttt cagcccccgg gtcgccatca ggtagtaaca acgtcgatca
    26701 tgatcggcca ggtagaccga acagacctcg gtatccatcg caagacagat gtcggtaacc
    26761 agaatattta acgcctcatt caggcgtggt gcgctggcta ccttttcgac tatttcgcgc
    26821 aggcgagtga gcataattgg cgtgacttaa cctcttttac gtcgataagc agatgcgttt
    26881 tgtggttttg gcgtattttc ctgcagtgac atcaccacac tcgcgaactc tttcattacc
    26941 ctacggtaga catcacgttt aaatgacacc acctgtctga ccggatacca gtaacttacc
    27001 catcgccagc cgtcaaactc tggtgtactg ctggtttgca tattgatttc tgcatcgccg
    27061 ctcaccagct gcaagagaaa ccatttttgt ttttggccga tacaaaccgg cttcgtgtcc
    27121 caacgcacca aacgtttcgg taatttgtag cgcaaccagt tacgcgttga agcaaggatt
    27181 cgaacgtctt tgcggcttaa tcctacttct tcaaacaatt cacggtacat cgcctgctct
    27241 gcggattctc cggggttgat tccgccttgc ggaaattgcc aggagtgctg accaaatcgc
    27301 cgggcccaca ttacctgccc ctggcgatta caaatcacga tacctacgtt tgggcggtag
    27361 ccatcgtcat caatcaccgg actacctcaa aataaagctt tatatacgaa tgattgtttc
    27421 atactccagg aagacggtaa accactctct gcagggcatt acacactaat aacaattgaa
    27481 taactcacag ttatgtgcag agttataaac agaggaaggg gtggatagcc gtttttattc
    27541 accttttctg tggatagagt tgtgaagaac tacggaatta ctacgggaaa acccggagca
    27601 ttctgaataa gcccgatata gacattttaa aatattcatt attagtcaat gcattacatg
    27661 taatttgagt aataaaaaag agtaattcgt gacccaggtc acacctctca tttacgggtt
    27721 ggcgaaagat caaccaatgc cgtatttatc cacagaatgt gccactaagt taagcactga
    27781 accactaaaa actggagttt catcgcacgt caaggctgta aatggaaaca gtagtggagg
    27841 tttttcacag ttatcccagc tttctgtgga taacatggtg taagatcctg tttattttca
    27901 gtgaccagat ttggaaaacc tattgcagtg ttgcgcaact cgattaccgg cagcctaaaa
    27961 agcaatataa atcagggaat tgaacaacac atgtggaaaa agttacactg cgaatattcg
    28021 gcacataatt gctgtttgtt ttttaatcaa ggtatcatga catgtcccaa cctcgcccac
    28081 tgctctctcc tcccgaaact gaagaacagt tgttagcgca agcacagcaa ctttctggtt
    28141 atacattggg agaactggcg gcacttgccg ggcttgttac gccagagaat ttaaaacgcg
    28201 ataagggctg gattggcgtg ttactggaga tctggctagg tgccagcgca gggagtaaac
    28261 ctgagcaaga ttttgccgct ctgggcgtgg aacttaaaac tatccctgtg gatagtcttg
    28321 gtcgtccgct ggaaacaaca ttcgtttgtg ttgccccgtt aacgggtaat agcggggtga
    28381 cctgggaaac cagccatgtg cgccacaagc tcaaacgcgt actgtggata ccggttgaag
    28441 gcgagcgcag catcccgctg gcgcagcgtc gcgtaggatc accgttgctg tggagcccga
    28501 atgaagagga agaccgacag ctacgcgaag actgggaaga attaatggat atcattgttc
    28561 tcggtcaggt tgagcggatc accgctcgtc acggggaata tttacagata agaccgaaag
    28621 cagcgaatgc gaaagcgctt accgaagcca ttggtgcccg gggcgaacgg attctgacgc
    28681 tgccacgcgg cttttatttg aagaagaatt tcaccagtgc actactggcc cgtcattttc
    28741 tgatccagta gccatcgctt tgacctgccg ctttccgggc atataattac cgcttcattt
    28801 ttttggcagg gctttttaga tgttatttgc atggataacc gatcctaacg cctggcttgc
    28861 gctcggtacg ctgacgctgc tggagatcgt tcttgggatc gacaatatta ttttcctttc
    28921 tctggtggtg gcaaagcttc ccacagcaca acgtgctcat gcgcgccgtc tggggttggc
    28981 gggagccatg gttatgcgtc tggcgctgct ggcatcaatc gcctgggtta cgcgcctgac
    29041 aaatccgctt tttacaatat tcagtcagga aatttctgcc cgtgatttga ttctgcttct
    29101 gggtggcttg ttccttatct ggaaagccag caaggaaatc cacgaatcca ttgaaggtga
    29161 agaagaaggg ctgaaaacac gcgtttcatc attcctcggc gctatcgtgc agattatgct
    29221 gctggatatt atctttagcc tcgactcggt gattacggct gtgggtctgt cagatcacct
    29281 gtttattatg atggcagccg tggtgattgc cgtaggcgtg atgatgttcg ccgcgcgctc
    29341 gattggtgat tttgtcgaac gccatccttc ggtaaaaatg ctggcgctct ctttcctgat
    29401 tctggtgggc tttaccctga ttctggaaag tttcgacatc cacgtaccga aaggttacat
    29461 ctacttcgcg atgttcttct ctattgcggt tgaaagcctc aacttgattc gcaacaaaaa
    29521 gaatccgctc tgatactccg tacgctctcc tgcgggaggg cgtactcact ccttttaccc
    29581 tcacagttta agattttcct gctttcaaga tatataacgt cggtttataa acagactatt
    29641 atcataggta ggccaaatga ggataaccgg atgaaaaaat gggcagtaat aatttctgca
    29701 gtcggactgg cgtttgctgt ttccgggtgt tccagtgatt acgtcatggc gaccaaagat
    29761 ggccgtatga ttttgaccga tggaaaacct gaaattgatg atgataccgg gctggtgagt
    29821 tatcacgatc agcaaggtaa cgcgatgcaa attaaccgtg atgatgtttc gcaaattatt
    29881 gaacgttaac aaataaggtc agcatccggc tggccttaag atttttctct ttcccttttc
    29941 ccttccctct gccattttta tattccttat gtcgtgatta taaaaaggaa acggctatgc
    30001 aatatcaccg tataccccac agttcgctgg aagtcagcac gctggggctt ggcacgatga
    30061 cgtttggtga acagaacagc gaagccgacg cccacgcaca actcgactat gccgtcgctc
    30121 agggcattaa ccttatcgac gttgccgaaa tgtacccagt acctccgcgc cccgaaacgc
    30181 aagggttaac cgaaacctac gtcggcaact ggctggcgaa acatggcagc cgcgaaaagt
    30241 taattatcgc ctccaaagtg agcggaccgt cgcgcaataa tgacaagggc atccgcccgg
    30301 atcaggcgct ggatcggaag aatatccgcg aagcgctgca tgacagcctc aagcgcctac
    30361 agactgatta cctcgatctt tatcaggtgc actggccgca gcgcccgacc aactgcttcg
    30421 gcaaactcgg ttatagctgg acggattctg cgcctgcggt ttcgctgctg gatacgctgg
    30481 acgcactggc agagtaccaa cgcgcgggaa aaattcgtta tatcggcgtg tcgaacgaaa
    30541 ctgcatttgg cgtaatgcgc tacctgcatc tggcggacaa acacgatctg ccgcgtattg
    30601 tcaccattca gaacccttac agtctgttaa accgcagttt tgaagtaggt ctggcagaag
    30661 tcagccagta tgaaggggtc gaactgctgg cctattcgtg cctgggtttc ggcacgctga
    30721 ccgggaaata tctcaatggt gcaaaacccg ctggcgcacg taatacgctc tttagtcggt
    30781 tcacccgcta tagcggtgag caaacgcaaa aagccgtcgc ggcgtatgtt gatatcgcca
    30841 gacgtcatgg cctggaccct gctcagatgg cgctcgcgtt tgtacgccgt caaccgtttg
    30901 ttgccagcac tctgctgggc gcaaccacga tggatcagct gaaaactaac atcgaaagtt
    30961 tgcatctgga gttaagcgaa gacgtattag ctgaaattga agcggtgcat caggtttata
    31021 cttatccggc accataagat taacgccggt taaaaccggc gttaaatatt aatgacggcg
    31081 ctgccagatc cacagcgccg ttattgccag cgcaaacagc gcaccgaagc caatgccaat
    31141 gggcacgacc gggatgccta tcattaccgc cagcgagtaa atgcccagca tcaacaacat
    31201 ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc cccgccccga cgcttttttt
    31261 accccgctcc tgtagcaacg cattgagcgg aacgacaaaa aagcccccca tcacgccaat
    31321 cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc agggaaaaaa tcagtaccac
    31381 cacgccaatc aaaatcccgg ctggcataca gcgtgacacg gtttccagcg taactaactt
    31441 cgctgccgca cctgcgccaa ccacgatacc aatcgctacc atcgcgttga gataggtggg
    31501 cgtagcgtta tcggtaatgc ccagcgccac cggtacccac agcaccaaca ggaaacgcag
    31561 cgtgacaccc gctccccaga ataaactggt gcccaccagc gaaaaacgcg tttcaccatt
    31621 gcgccatagc gaggtgcagg cattcaggaa actgcgggtc atgttgatga gattccagga
    31681 ctgccccgga cgcgccgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    31741 gtaggccagt gcgcatgcgg ccagggcgac gaggacatgc cagtcagcca gcacaccacc
    31801 ggctacggaa ccgagcaaaa tcgccgctat ggtagaagct tccattaaac cgttagcttt
    31861 cactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    31921 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    31981 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    32041 atccgccacc tgcccgacaa acggcgcaaa aagaatgtaa gcacctacaa acaccatttg
    32101 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa
    32161 cagtagggca ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    32221 cttcgaccac aacgaagtgt tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt
    32281 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    32341 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    32401 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    32461 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    32521 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    32581 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    32641 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    32701 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    32761 cttcgatacc agggaccgac aacaggcgcg catccattcc tggtagaata cgccctaccg
    32821 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacgc
    32881 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    32941 gtaatttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    33001 aacgcgcgta gtgaccgagg aaagtcgagg tgccgaacaa cacggtgcaa ctgcggtcat
    33061 acaccaactc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    33121 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaacg
    33181 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacattcg
    33241 ccagaatgct tttatggcta tggacgacgc ctttcggatg gccttcagaa ccggaggtaa
    33301 agaggatcag cgcctcttct tccggctgct gtttaacctg tgccagacgc ggcatcagca
    33361 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    33421 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    33481 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    33541 ttaccccggc agtgtagttc atcattgcgg gcatgcggcg acgggcgatg gccccaaaaa
    33601 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    33661 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    33721 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    33781 tcagtaaaga ttcgtacagc gtttcacgcg ggcgcaccgc cattcgcgct tccatcatta
    33841 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    33901 tcgccacctg cgttggtggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    33961 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    34021 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    34081 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    34141 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    34201 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    34261 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    34321 ttagaacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    34381 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    34441 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    34501 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    34561 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    34621 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    34681 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    34741 tcatgtgctc aacccgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    34801 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    34861 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    34921 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaagaa tgtaagcgtt
    34981 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    35041 agtcgccacc gtttcccgcg tcattaataa ttcacctaaa gccagtgaag cttcccggct
    35101 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    35161 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    35221 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    35281 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    35341 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    35401 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    35461 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    35521 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    35581 tctgcaaggg tattacgatg cccttgctga aagtggtatt gcggccaatg accggctggt
    35641 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    35701 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gtgcgatggg
    35761 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    35821 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtgac
    35881 gatggcgacc caggctgccg aactggcttt ggcgctggcg gataatcgcc ctctcccgga
    35941 aatcactaat gtctttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    36001 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    36061 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    36121 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    36181 catgcagtac cagataatca cccgccttca cttccggcaa ggcgcgggtt tcaacatttc
    36241 ccccttcctg ctgggtaaag acatcgcccg attcacataa tggcccggcg acgacggttt
    36301 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    36361 aactaccgta cattgccggg cgcatcagat cgttgaaccc agcatcaacc agcacaaagt
    36421 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    36481 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgggcgattt
    36541 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    36601 gttgataagg aacagaaagc ccaccgcccg cagagatagc ctgtaaatcc tgaccgaatt
    36661 cgatgacctg acgcaccata gcaccacaca cctgttccag atgggcataa tcaacgccag
    36721 aaccaatgtg catgtgaatg ccgaccagct gcagatgatg acgttgtatc acgtccagtg
    36781 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    36841 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    36901 aaaccaggcc cagttggtcg agcatatcaa cagaacccgc attcaccgga atttgcaatt
    36961 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    37021 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    37081 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    37141 tctgtgcaaa gcgcaccaca tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    37201 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    37261 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agataagtgc
    37321 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    37381 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    37441 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    37501 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    37561 ggctaaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    37621 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    37681 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    37741 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    37801 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    37861 gactcactga gacactccat acgcctgcgg gaacagaacg taccgaatta ctctctttag
    37921 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    37981 atgattttca gggtgagaac tacatcagcc tttcccgtac tgacagctat cgccagttgc
    38041 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    38101 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtgt ttcggtggtt aacccgctca
    38161 ccgcactgga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    38221 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtg caggcgttta
    38281 gtgagcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    38341 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcag tatcaaacac
    38401 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    38461 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    38521 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    38581 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    38641 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    38701 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    38761 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    38821 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    38881 ctggcactca tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag
    38941 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    39001 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    39061 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc cgatctctat
    39121 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    39181 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    39241 tccggaatga gccagaaagt gatgcccaca aacgcaatgt tcagcgcagt gtagagccag
    39301 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggtcgcgcc gataatcata
    39361 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    39421 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    39481 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    39541 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    39601 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    39661 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    39721 gccattttga agatacgcgg cgcatagtac atgatgatgt tcataccggt aaactgctgc
    39781 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    39841 agtgcccaac cgccttgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    39901 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    39961 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcaggact
    40021 gctggtaaag caagaacccc caacattgcg cgccagttac cgctataact gaacgctgta
    40081 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    40141 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    40201 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    40261 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    40321 cgacccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    40381 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    40441 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    40501 gctaccgaaa caaacatatt catacgccgc gtatcccgca aagaacgtgg cgttaaagca
    40561 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca tagtgaaaaa
    40621 atacgtgaac aactcacgca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    40681 tgcgacatgt cgttatgtga tggatattcc aattttcaaa ttaagttgaa ttattgagat
    40741 tattattaac cacctaattt tacagcagat aaaattcata aagttcat
//
LOCUS       DAZFWP020000020        40741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFWP020000020 DAZFWP020000000
VERSION     DAZFWP020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.020"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(61..1065)
                     /gene="argF"
                     /locus_tag="ACHK0Z_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             complement(61..1065)
                     /gene="argF"
                     /locus_tag="ACHK0Z_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU4501068.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1227..1643
                     /gene="rraB"
                     /locus_tag="ACHK0Z_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             1227..1643
                     /gene="rraB"
                     /locus_tag="ACHK0Z_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU4501069.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1689..2192)
                     /gene="yjgM"
                     /locus_tag="ACHK0Z_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             complement(1689..2192)
                     /gene="yjgM"
                     /locus_tag="ACHK0Z_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4501070.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSQSDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2385..3581
                     /gene="yjgN"
                     /locus_tag="ACHK0Z_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             2385..3581
                     /gene="yjgN"
                     /locus_tag="ACHK0Z_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGU4501071.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3637..6492)
                     /gene="valS"
                     /locus_tag="ACHK0Z_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             complement(3637..6492)
                     /gene="valS"
                     /locus_tag="ACHK0Z_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU4501072.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6492..6935)
                     /gene="holC"
                     /locus_tag="ACHK0Z_RS05040"
                     /old_locus_tag="pgaptmp_001008"
     CDS             complement(6492..6935)
                     /gene="holC"
                     /locus_tag="ACHK0Z_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU4501073.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7069..8580)
                     /gene="pepA"
                     /locus_tag="ACHK0Z_RS05045"
                     /old_locus_tag="pgaptmp_001009"
     CDS             complement(7069..8580)
                     /gene="pepA"
                     /locus_tag="ACHK0Z_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU4501074.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8734..8784
                     /gene="ytgA"
                     /locus_tag="ACHK0Z_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             8734..8784
                     /gene="ytgA"
                     /locus_tag="ACHK0Z_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU4501075.1"
                     /translation="MSIIILATISTTQENK"
     gene            8847..9947
                     /gene="lptF"
                     /locus_tag="ACHK0Z_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             8847..9947
                     /gene="lptF"
                     /locus_tag="ACHK0Z_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4501076.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9947..11029
                     /gene="lptG"
                     /locus_tag="ACHK0Z_RS05060"
                     /old_locus_tag="pgaptmp_001012"
     CDS             9947..11029
                     /gene="lptG"
                     /locus_tag="ACHK0Z_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4501077.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11148..12650)
                     /gene="yjgR"
                     /locus_tag="ACHK0Z_RS05065"
                     /old_locus_tag="pgaptmp_001013"
     CDS             complement(11148..12650)
                     /gene="yjgR"
                     /locus_tag="ACHK0Z_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU4501078.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQKNPPTKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12780..13799)
                     /gene="ahr"
                     /locus_tag="ACHK0Z_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             complement(12780..13799)
                     /gene="ahr"
                     /locus_tag="ACHK0Z_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU4501079.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            13995..14079
                     /locus_tag="ACHK0Z_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     tRNA            13995..14079
                     /locus_tag="ACHK0Z_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14029..14031,aa:Leu,seq:caa)
     gene            14266..15510
                     /locus_tag="ACHK0Z_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     CDS             14266..15510
                     /locus_tag="ACHK0Z_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4501080.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGNGMHLLVHPNGSKYWRLQYRF
                     GGKQKMLALGVYPDVSLADARARRDDARKLLANGIDPGDKKKNDKIEQEEARTFEQLA
                     IEWHATNKKWSEEHSRRVLKSLEDNLFPAIGKRNIADLKTRDLLAPIKAVELSGRLEV
                     ASRLQQRTTAIMRYAVQSGLLDYNPAQEMVGAVASNNRQHRPALQLKLIPELLRRIDS
                     YTGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETSMWTIPAEREAIEGVKHSQRGS
                     KMRTPHLVPLSHQALAILKDVYKLSGDRDFVFIGDHDHRKPMSENTVNKALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERSSVRAAYIHKAEHLDERRLML
                     QWWADFLDANRESEVSPYDFGK"
     gene            16050..16901
                     /locus_tag="ACHK0Z_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     CDS             16050..16901
                     /locus_tag="ACHK0Z_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501081.1"
                     /translation="MLVNSWCNALNEAISTSATVCAEDLYKGGHWATAKAILDDYPID
                     LWILSAGLGLLHHKDNVVPYKATFAVGYDESIPLYSQEYVGKSFHRTWWKEITNRSIF
                     KSKHPTSIVELMKKRKRDYYIICGSPDYINAIELDIINGLEYLVDAKKQLLIITSKKI
                     NGRLTTYLFKTNQNMAQWLRCNMLMLNISVAKYIVKEFTSKQLNDLNELSQKLKEELK
                     ELPEREVKKGIRRSPEEVKSFILKIMEKNPGISATHALREFRDSGNSFEEKRFRAEFM
                     ALREAKP"
     gene            16975..18225
                     /gene="dpdA"
                     /locus_tag="ACHK0Z_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     CDS             16975..18225
                     /gene="dpdA"
                     /locus_tag="ACHK0Z_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase DpdA"
                     /protein_id="HGU4501082.1"
                     /translation="MSKLKYFFPDSQDFIDPSFDFFRETRNEHRVRQRDDQYPHEVFP
                     HPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPENMETMGDCGAFTYV
                     NQDVPPYRVEEVIEFYETSRFNHGVSLDHIVFGYEKPGEVFSGEVLTECRRRQDITLT
                     LAQEFLNKSQKSCFTPFGVAHGWSKTSYRQSVEALLAMGYKNITMGGMVPLKTAQILE
                     TLEEIKPLLKSDTRVHLLGIARPESFSDFIKFGVTSIDSTTPLQQAFKDRKNNYHTLD
                     GPAYTAVRVPQFDANPSLSRKIKSGVIDQDIARHLEKDAMNALFEYDKGALSLEKALD
                     AVLAYERLHSGEKEADKIRADYERTLGERPWKQCQCNICQAIGINVIIFRGAERNRRR
                     GFHNIQVLYNRLQHTLSLRSEELS"
     gene            18222..19352
                     /gene="dbpB"
                     /locus_tag="ACHK0Z_RS05095"
                     /old_locus_tag="pgaptmp_001019"
     CDS             18222..19352
                     /gene="dbpB"
                     /locus_tag="ACHK0Z_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein DpdB"
                     /protein_id="HGU4501083.1"
                     /translation="MSEYRVPALRIRQGEERQLYSLAIEGKQISKIAAISRIRRGEES
                     LVGYQRPEVRNHIREIQRYIESANPMIPNPVIIAFDKRVRFEPLTDNDDMGHLVIPFS
                     EDKDFEKPGFIVDGQQRTAALRDAEINSFMMPVSAFIANDAEEQREQFMLVNSTKPLP
                     KTLLYELAPHTHGRLPSDLQMRKFPSLLTQRLNFGEGSLAGRIKTATNPDGVIADNSM
                     IKMIDASLREGALYRFRDPATGLGDEEKMVKLLNNFWSAVETVFTDDWDKKPRYSRLL
                     HGVGILALGSLMDEIDQVHQDYKGEPGWTEIPSYTRFVEELNRIKPLCAWSSGVWNFG
                     TDIDGQPIVRKWNELQNLSKDISLVTDYLVTGYMKTVNADIK"
     gene            19789..22944
                     /gene="dpdE"
                     /locus_tag="ACHK0Z_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     CDS             19789..22944
                     /gene="dpdE"
                     /locus_tag="ACHK0Z_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdE"
                     /protein_id="HGU4501084.1"
                     /translation="MPLEVGDLVDSPYGIGKLIRINNNDALIKYFDSPVNYEGIEQTV
                     EVKHLKKAELHAESLVYRYDYEVAHWQIARVVGAVPGGVRLHFPNKQQEDVSLNDIFV
                     RWDRPISDAAALLAERITYTPFWQDARLAYQHQMLKQRNACAGITSSLSSSIELEPYQ
                     LAVARRVLSDPIQRYLLADEVGLGKTIEAGLIVRQHVTENPLAHCVVIVAPGVLHRQW
                     QQELSARFHLEELLNKSIHIIDMAAFSTADPLSFRPDMLVIDEAHQVGAWAWDAEYEQ
                     RYASVNYLAQQSEKLLLLSATPVTGNEKNFLAMLHLLDAKNYPLTDTGLQKFQQRIQQ
                     REEIGSLFYAFNCDNDNMTLEENLEALTRMFPEDEVLNGMASQLLPHLDMMQPLRSEA
                     RDLLINKIRHYIGNRYRLHHRLLRNRRSNPAISVLMPGLSGLSLKKYATTNASNDMMI
                     AWRESAIMMHPQDPTLAAIYQLLFEAAIATPELLARLANYRLQQESLGAAQGFLYSDS
                     QLALLQHPLFDGEKEILLELIDRCKMECDSKRQRLIDVLEKLLDSNVRELPYIVVFCD
                     QPWVADQVFTDLMIPFIGKIQRHNPDKEVRFGKGSKCQILVCDRRAEEGVNLHGGRKI
                     ALHYDLLLSPNRMEQRLGRLNRYCATESATTILSMALVSEAEGLQNQWLTILNEVFDV
                     FEESIASLQYLIDEELQRLISNLYPLGEESMMQLSARLAGEDGRLTVEKQRIEMQEVL
                     LQIDGDIIEAREYADALAEIDENEEEFRRCARNLIGNVLKFDFIPDEKDPSIFRYRFN
                     PGDRGTGTLVSPQRLQKHCLLGFDLNQSSGSSPMTWPLSYYRADTSQGKIRPARLGEP
                     FHDAILQMLDFEERGVASAIIRITPGMGDENIFFRYDFLIEADVHTPALQRLADHLMP
                     PETITLWLDQAGQQVTNAEVLMILNEDYKPKDKGGRHLNLNEERWAQISHCISAKDWR
                     TWCNDSYSIAQRLAVEQFATQQALSLSRLEHYLSSAALHHANRQETGQMLRQGIAQPK
                     MTCLATRALIIASEAILDEDS"
     gene            22950..25517
                     /gene="dpdF"
                     /locus_tag="ACHK0Z_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             22950..25517
                     /gene="dpdF"
                     /locus_tag="ACHK0Z_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdF"
                     /protein_id="HGU4501085.1"
                     /translation="MNLAQLIDNDFISSRLSECLLPEATAGHLDRRALIRDWLYREKH
                     AHHNDNELTVPDDSQPGWPRQEEWEASGINCQRLGQTFLLSAGPAWCPQWLEQGNGMP
                     FLASDQHQFCRQDTRVPMDYVLRRYFKKDNYISYQAPGQRDAVRRALFAPPGSTLLVN
                     LPTGTGKTLVAQALALTASAPGALCVLIAPTTALVLELASRFRKLLIEAGNKAESVTG
                     WYSGLPAQDKYDTYMRIRVGEQRLIVTSPEATCSSLQFALFEAAKQGGLRHVIVDEAH
                     IVASWGNDFRPHFQQLGGLIRGLKRVSREANLTPCSTLLMSATITEASRKILRDTFDQ
                     EMSELHACHLRPEPAYWCYRAKDHSDKRDKVLQSLAHAPRPLILYVTRPKEAEEWAQQ
                     LRLQGYQRLATFTGDTPSEVRRSLLEKWDKNQLDIMIATSAFGVGMDKNDVRTVIHAT
                     VPENMDRFYQEVGRGGRDGYACTSLLIYNHQDVKQAEHLSSQKLIGLELGLERWKKLW
                     GSAESVGDNLYRIDIERIHSGLNFRSKQNKLWNLRTVLMMVRAGVLELDADLQNPPDK
                     KDFADVNRYEAAKEQIIRQNGNSLVVRILRSHHTGEELWKTRIIPSREETLQAARANH
                     NALINWLHTPLSRPLCEALVDLYSMAEFIPGHACGGCPACRDNQALTSNYPSPPTLLL
                     NPPASVAQQALSRWQALSGLSYSSCFITYPPVNDDEWERENWYHRIATLLVQLYHRGI
                     VQRVWADPALLHSIFSAIPRGERLILLSDELPARDEPAPDNDVMAELAELVLPTQESC
                     ANGYLPPFNPAPLQLTLIPHDMPDPHHPGGAHYVLLDNHNWITIEQLQRKLDSVDYQ"
     gene            25501..26406
                     /gene="dpdG"
                     /locus_tag="ACHK0Z_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             25501..26406
                     /gene="dpdG"
                     /locus_tag="ACHK0Z_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdG"
                     /protein_id="HGU4501086.1"
                     /translation="MSIINNAHGGSQYAVLAAFYQVLKASPKNQLPREKLMALCAPET
                     LQTGDSQNARGKASQELRAWLDLGLLTEEKVEETTQISFNANYFSTKSMPLRQAARRC
                     LQAQENNSDLSSRDERAVDLTLLAALLLALDVYQNAEIKSGNLEVLVNTFIPDFRINS
                     NEIAVVPGYLSWLGFAQQLTRDSYAIDPTDSIREELPFLMRVGEQLSIGEVLQRLNRA
                     LPVLDGGSYRQQVEERISLYGWHSLSPNRLSTSLSRALLRLQLSGVINMKAESDASGA
                     MQLTGRNGSVLRTVTHLTLNDVGVH"
     gene            26406..29462
                     /gene="dpdH"
                     /locus_tag="ACHK0Z_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             26406..29462
                     /gene="dpdH"
                     /locus_tag="ACHK0Z_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdH"
                     /protein_id="HGU4501087.1"
                     /translation="MPQLQSYWPSEQNVTACILTEAESLADSQLLAVHEPMRLDRIEF
                     QSGKVTQVGEGALLDFLLEHNRPLPLIGASGVGKSHLVRWVHAQLKRREDHAKYHIIR
                     IPKNASLPRVLAAILEGLDGEEYQSIREKVNGVGKQLIPENVAEHIALKLRQALNAAY
                     TDAIEELRQEKMGKIQLEESRKKQLEEIQQHAASSRLPALFFDSVMTQYFTAPGACLH
                     NIAQRFCQGANDDSIANLRYEMSAEDFTFKGLDFRQVSPTVLPYLRNQQLLTSDEKKQ
                     AAARVVNVVRSQALEDTFVELFSFNRANFQDLMRMIRRQLLAEGRDLILLVEDLTATS
                     AIEDVLIACLIEEDEYDGKKVLCTLRSIIAVTEGHDSFKRLRNTLGTRARYEWVIQQH
                     ASENDVALKKRVIDFCGRYLNAARHGAAALETYHLQQDAQCYQEIPVWQDQEVLENES
                     AAPALASFGSSSIGHPLFPFNPAAIGQLVERHCKVKEQGLVFIPRNILHEILREPLKK
                     YRQNYLDGQFPPSQFAEIVCNPALQQRVRLEGLSQPERINSLLAVWGGNSTNLAGLTP
                     AICKEFGLPDAAVLLGGGGTPPEPAPEPVPEPAPVPESVPAPPSPLDEWKLVLDKWHQ
                     SKTLEQTRARDIRKWLLEALNARIDWSTELIQTSLMASSAKTQGRIHLPGVLVGNQSH
                     PLVKLEDTPELRSACLAMAHFNHYQSWDYDGSEIDYAAFHSFMDCIEHEVKLKVLQEE
                     REELSSIINDLQLSGAFLGLDGLSASHPATLVQALLTEPQVANSNHPLLDEFLTQARD
                     TVIDEHLKRRERLIALTAAKKPGAQPYAINGLMLVEASKKQSPDAGKQPGKIHEVKSK
                     LGGYRTLLLKTQESITERLSDGNSASLAAVNLCDTMKEIVELANSADVARPMGETVPV
                     LLKLLKSWKDPELKKLIRQLEKLTLPADNSHMLMREILAINGEQFEAFRQLLENWGKF
                     EDATDKYVQQRISDKGGDQVEDAENQVTDTLANLAEQLTLLEADLS"
     gene            29459..30250
                     /locus_tag="ACHK0Z_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             29459..30250
                     /locus_tag="ACHK0Z_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501088.1"
                     /translation="MSEVNINDVVTPVASVPEERGIFARRSGDFKARLQRYQQMEKAQ
                     DIRDNLLRIAKKIDTPTQTLAQALCSYRVLRQVHDDETGKPIVGPFDRATFGNPQILL
                     TAFCQQYATHKHDVEQEGELALLTQEIRRLASELSTHTTAAWVNYIRGLKAQWEVDQR
                     LFSNLAHQEGQRKVQQHYIDLVEKFTSSSRGLPKTIEEFETVRDLHEKLCQQRGALKL
                     DVPEEVNHFLKAVAGQGATLDMLTPNVLSWLTEEDDPTRYRIRRL"
     gene            30266..34795
                     /gene="dpdJ"
                     /locus_tag="ACHK0Z_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             30266..34795
                     /gene="dpdJ"
                     /locus_tag="ACHK0Z_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdJ"
                     /protein_id="HGU4501089.1"
                     /translation="MEQIIRAVLDGLESQEAVLLTWGNTQGFFTETELRNTISQVLQN
                     HPLDANGLTSFGIVKVMIDRGLLLRHQQNGVSVFRTRMAESVRLYSQLRQMFPKHGFN
                     DNWRTAPTLVSDYRFLRRQRKYPKRDRQAAEVLQQLDEIAGFTSTHRQLLSALLSHSS
                     GMYNLAYFQQEACKNVLRGLQQSQHRSTATIVCAGTGTGKTLAFYLPALTFIGSELVA
                     KRKDAVKALAIYPRNELLKDQFIETLRQTRKLNTELAKKGVRKVRIAALFGMVPTNKE
                     AIHRDYMKDAWCRHRNGMACLYIPCPTESCRGKMVWHTADYDKNLERLHCDSCSQTIE
                     PDEVILTRKRMKIELPDILFTSTEMLNKQMGNPFLASLFGIGKNVVPPSMMLLDEVHT
                     YSGVSGAQNGMLIRRWRHLSGATPHFVGLSATLEQATRFMASLTGLEEYLVAEVSPIE
                     DDMETEGAEYMLALRGDPASRSSLLSTTIQASMLTRRMLDNAAVPVSNGMYGTRSFVF
                     TDDIDVINRLYFQLLDAEGRYSNGNINAKKEPLAMLRGDAPNEEKFTFGQQWPLAKMI
                     GHTLDSADRSNVKRTSSQDAGVDHAADLIVATASLEVGFNDPNVGAVIQHKAPRDNAQ
                     FLQRKGRAGRQRTMRPWTVVVLSDYGRDRMAFQCYENLFEPVLKARQLPVGNSYVLRM
                     QAAFATMDWLSSRNEYLNQWNRGIWDDLSVPQDKGKPSEAQSKLADLIEDLLNSQKIQ
                     QEFNSWLAEALGIKDEKLLQSLLWQPPRAIMTAFLPTVLRRLRSNWSRLGIEQADNCR
                     KSTPMPDFIPSALFNDLCLPELQINLLGEVGQEPDAHSMPILQGMKDFAPGRISKRFA
                     IKNIRECHWLVPKKLELKDGSHPFPIDDYCPPDKRESMPDCHITTRTGMQVIPCFRAW
                     EVTASTPPDDLKLSETSNAFLNWHSEIRPPQNGIPAEVPSNNAWQDIFQQVEFYSHQQ
                     HCPIEAVRFATGSRANIKFSDQREDLQIDFKFEHQGEPAAFGFSLWVDAIKFQCRLPN
                     FDLASISNNRELVAGLRTARFLYEVSHDEAISVNTFLGGWLAETVLAGIGSEAVLKNC
                     TPEQAFRAMEHNSARIALIDVPARIFQTLYDAQTGEEIEQDLQQSLRELLSQAPVMAR
                     IADWVELLWQPLDNTWLEWLTLNFTNTLGAALLQTAMQLCPDADDNDLILDLNGGPVR
                     TSLLAPDQREIWLSETTVGGGGIIEKIQQIYREDPRSFFELLDFNLSPGDYETMDNNV
                     WHLLQTMVNPTSSLPACMNEMRLANDHASQVQAQRNLLGELQRSGFMTSHSFLSAINT
                     RLLRPGTDASSDVFLLQLQQDWRQQEQRLGIELPQRVYAFTCSQSSALDQQLQQASQG
                     QHNLESWRLNTCNGLLWPRGHTIRDSWLTWYNPYSFAGPTERLLLAHVVQHSCAQVAL
                     SQPDWMQQCHQHLENNGKCELLAEPGDAINEAIATLLVTPVEMYGLFFYPRVSALRRH
                     NNDTFAMIEIPEAIQ"
     gene            34792..35334
                     /gene="dpdK"
                     /locus_tag="ACHK0Z_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             34792..35334
                     /gene="dpdK"
                     /locus_tag="ACHK0Z_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009114670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein
                     DpdK"
                     /protein_id="HGU4501090.1"
                     /translation="MNTRRIFKSRTSRQSEVLDVLSSLLVAEIAQPSAELWIVSPWIT
                     DLDLLDNRTGRFDYLEPAWGQRQVQTSELLARAVANGGTLKVMTNETQHNARFIRQLT
                     ERVANYGMLDNLYIGQKEVLHTKGFLGDHFFLSGSMNLTIGGIVINDEQINLTTDKTA
                     IVQALLAFNDFYQPAQGGVQ"
     gene            35331..37586
                     /gene="dpdD"
                     /locus_tag="ACHK0Z_RS05135"
                     /old_locus_tag="pgaptmp_001027"
     CDS             35331..37586
                     /gene="dpdD"
                     /locus_tag="ACHK0Z_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdD"
                     /protein_id="HGU4501091.1"
                     /translation="MNSADSEKFLRAFFHRENNIISWEKIAGGLLEASTQQRLTPWLD
                     DVRNGCFPVILPRVVKGSNDKVTWYAMADEERDYAELRELLDAHVGTSWSDFNRQRTM
                     LDPSDPVEKALIDHYSDRVYKFSSLSLSRIPEIFNGIERLRQQLASRPSYQLTLLRPR
                     GRILRDIRAALRRRDVPQTRLYLQELREAGGIGHINELFIELRCMALEGDAKSLLDST
                     QLRQVLDMPHPLVLAEEVMTVFYQQFLLKYEQLSDLAAQRQAFNNLLVKNVPRLFQVT
                     QGINTPSAIKNILLWLLSDTQSDPARLREQAQQVVAGMKGATDDDRHWIRRLLEAFPT
                     VAVASPPTASIDELLARADEYPESVLKELLLLPVSARGVAIALQCAWVVGSAGAAVSA
                     VSYLQQLAQQERDLLLAKPAFQRYYTALQLMLPSKRTEHDTAGINNWQDWLKEVLNNP
                     QWSGAIEAVEQACGQWNAAAFQPEAFVSLLDKTNNCASMVLRDAIPVLLEWLDNHQTS
                     ASWQSVRLALLQQLALDDNSSREDIFTACELARGYLEGDSNAHDYRELIENLQMMIES
                     SGTTSASAWLDLLELFLVYPTADQLAREQLFNLVLNMFYRNPARYSELQWSLLAQFAG
                     ECGLVCEIPPQILEDSGEKNDEVRVGSTLNDKVVGIYTLTESVAQRVKKQLLADYPQA
                     DIRLNNDKGGSTMLRSLAREADVFIFAWLSSKHSAYYAIKEERGDKPLLQPSGKGSTS
                     MLDCIRSYLKL"
     gene            complement(37643..38005)
                     /gene="queD"
                     /locus_tag="ACHK0Z_RS05140"
                     /old_locus_tag="pgaptmp_001028"
     CDS             complement(37643..38005)
                     /gene="queD"
                     /locus_tag="ACHK0Z_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4501092.1"
                     /translation="MKTTLYKDFTFEAAHRLPNVPEGHKCGRLHGHSFVVRLEITGEV
                     DKHTGWIIDFAELKAIFKPTLDRLDHYYLNDIPGLENPTSEVLAKWIWNQVKTQLPIL
                     SAVMVKETCSAGCVYRGE"
     gene            complement(38305..38597)
                     /locus_tag="ACHK0Z_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /pseudo
     CDS             complement(38305..38597)
                     /locus_tag="ACHK0Z_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            complement(38768..39343)
                     /locus_tag="ACHK0Z_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             complement(38768..39343)
                     /locus_tag="ACHK0Z_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4501093.1"
                     /translation="MQNNQRWEPDWFLQSLLNNHIDKMVERYSCLRAFRTDLFYQDHT
                     AKFFLQDHQQLEWDIRKLMNRMMQLEAVVGFFWVIEWTEDHHYHAHVVFWLDGSKTQN
                     TYTWAQQAGLYWSDITDREGWYHRCEFKEHYEANINIPVRYSDPDSITNIRQVLAYMT
                     KIQQKHGLLLYGCNEVPARPLTGRPRSSQSL"
     gene            complement(39542..40135)
                     /locus_tag="ACHK0Z_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             complement(39542..40135)
                     /locus_tag="ACHK0Z_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501094.1"
                     /translation="MKDYKPRYEELYRYYQVWLTDFTKLTVRSGMCHQNIYHHHTLTV
                     GSLKFPGEEEVIAIVPQCLHRIIYGRAAAPLTVNEDLSVSLFTQEHLLTHHPMLEGIL
                     LSECVRLKQRPLANKLISLFRQFSGSELRLKLVWLCWYDLMLGNCLDDWTDNLRFKKD
                     KEMDIWINNRQAENPALTIMMDEYVCFAWRTTAEPLS"
     gene            complement(40232..40438)
                     /locus_tag="ACHK0Z_RS05160"
                     /old_locus_tag="pgaptmp_001032"
     CDS             complement(40232..40438)
                     /locus_tag="ACHK0Z_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4501095.1"
                     /translation="MTTRPSLTEDQFVDMAFITSLLQMTDKWIYKLIKDGVFPKPIKL
                     GRSSRWLKSEVENWLQERIDQSRK"
BASE COUNT        10079 a        10202 c        10405 g        10055 t
ORIGIN      
        1 ataaaataat gtgatgccgg gatgtttttg tatttcccgg catctttata gcgatatcaa
       61 ttatttactg agcgtcgcga ccatcaccgc tttgatggta tgcatgcggt tttccgcctg
      121 atcaaaaaca atgctggcgg cagattcgaa gacctcatca gtcacttcca ttccgccatg
      181 taggccaaac tcttccgcca ttttcttacc aagcgtcgtc tggtcgtcat gaaacgcggg
      241 caggcagtgg aggaatttga cctccgggtt accggtcaac tgcatcatct tgctgttcac
      301 ctgataatca cgcagtaatg caatccgttc tgcccatttc tcttttgctt cccccatcga
      361 cacccataca tcggtataga taaagtcagc accttcaact cctttggcta catcttccgt
      421 cagcgtaata ttcccgccat tttgctgtgc cagggcgcgg cattccgtaa ccagcgcagc
      481 ttccggccag catgcctgtg gcgcgaccag acgcaaatcc agaccggtaa gcgccgcagc
      541 ttcgagcatc gaattgccca tgttgttacg tgcgtcacct gcatagacca gcgtcatttc
      601 gttgaatgct ttaccgggta aatgctcctg catggtgagg agatccgcca gcaactgcgt
      661 ggggtgaaac tcattggtca ggccgttcca taccggcacg ccagcgtatt ccgccagtgt
      721 ttcgacaatc tcctgaccat agccgcgata ctgaataccg tcatacatgc gacccagcac
      781 gcgggcagtg tctttaatcg actctttatg accaatctga ctgccgcttg ggccgagata
      841 agtaacgcga gcaccctggt catatgcggc aacttcgaaa gagcatcggg tacgggtcga
      901 gtctttttcg aagatgagcg cgatgttttt accagtgagt ttggcttctt ctttgccgct
      961 tttcttatcg gctttcagct tcgcggctaa ctgcagcagg ctgttgagtt cagctggcgt
     1021 gaaatcgagt aatttcagga aatgcttatg ataaaacccg gacatagatc cctcctgtgg
     1081 ctaacgcctt aatgaattaa aattcaattt atatggatga ttattcattt gcaagtctaa
     1141 agcataaatc tttgtcacaa aggtggaggc aatgtcagtg gtgtgtgaca ataagagtat
     1201 cggcaggaca ttaagaggaa tgagccatgg caaacccgga acaactggaa gaacagcgtg
     1261 aagaaacacg tttgattatt gaagaattac tggaagatgg cagcgatccg gacgcgctgt
     1321 acaccatcga acaccatctt tccgcagacg atctggaaac cctggaaaaa gcagcagttg
     1381 aagcgtttaa actcggttac gaagtgaccg atccagaaga gctggaagtt gaagatggtg
     1441 atatcgtgat ttgctgcgac atcctcagcg agtgcgcgtt gaatgccgat ctgatcgatg
     1501 cccaggttga acaactgatg acgctggcgg agaaatttga cgttgagtac gacggttggg
     1561 gcacttactt tgaagatccc aatggcgaag acggcgacga tgaagatttt gtcgatgaag
     1621 acgatgacgg ggttcgccac taattaattg attaaaatgc ccgatagcac acactagcgg
     1681 gcattttttt agagttcgcg tagcatccgc acttcacaat ctacatggcc cgtgcagcca
     1741 agtgcatagt cgatatgttg aaaacctaaa tgttcataca aaccaatcgc ttctttcaaa
     1801 aaagcggtcg tttccagata gcagcgtttg aagcccatct ctcgtgcctg ctccatcgcc
     1861 attaaggcca gttttttcgc cagcccttta ccgcgaatag ccggaagaaa atacatcttc
     1921 tgcagttcgc aaatgtccga ctgactcccg gttaatggcg caatcccacc gccgccgacc
     1981 acttcaccat cgtactcaac cacccaatat gcatgacccg gctgactata cacttgatac
     2041 aactcgtcca ggttcggatc ggcgacggtg tagcctttat cagcggtaag accgtattcg
     2101 gcggatacct gacgaatgac gcgggcgatg gcgggattat cctgctgcgt caggcgacgc
     2161 attacaggtg attgcggcgc aatgttattc atagtttaac ccaataaaaa taaagtaatt
     2221 atacatttgt ttaataccac tctcttctta cgtgcgcaag cgacttataa agtaataaag
     2281 tttttgccag cctattactg ataagagtat tgtaacgtgg cagaacattt tactgtctgt
     2341 atatataaat tatatcaatg gattttttaa ttcagggaat ttttatggct caagttatta
     2401 atgaaatgga tgttccgtcc cattcgtttg tttttcatgg tacaggtgag agatattttc
     2461 ttatttgtgt ggtgaatgtg ttgttaacga ttataacgct aggtatctat ttgccatggg
     2521 cattaatgaa atgtaagcgt tatctctatg ctaatatgga agttaacgga caacgatttt
     2581 cttatggaat tactggtggg aatgtttttg ttagttgtct tgtttttgtt ttttgctatt
     2641 tcgcaatctt aatgacagtg tcagcagata tgccacttgt tggctgtgtt ttgactttgt
     2701 tactgttggt tttgcttata tttatggcag caaaaggact gcgttatcag gccttgatga
     2761 ccagtctcaa cggcgtaaga tttagtttta attgctctat gaaagggttc tggtgggtaa
     2821 cctttttctt gccgatttta atggccattg ggatggggac tgttttcttt atctcgacaa
     2881 agatgctaca tgccaatagt tcaagtagtg ttattatatc tgtggttctg atggcaatag
     2941 ttggtattgt ttccattggt atttttaatg gtactttata tagcctggta atgagttttc
     3001 tctggagcaa taccagtttc ggtatacatc gtttcaaggt gaaattagat actacgtatt
     3061 gtataaaata tgccattctc gcatttttag ctttattacc ttttctcgct gttgctggtt
     3121 atattatctt cgatcaaata ttaaatgcgt atgatagttc tgtgtatgca aatgatgata
     3181 ttgagaattt acagcaattt atggaaatgc aacgtaaaat gataatcgcg cagttaatct
     3241 attattttgg gattgctgtt agcaccagtt atttaacggt gtcgttgcga aatcatttta
     3301 tgagcaacct gtcactgaat gatgggcgta ttcgttttcg ctcaacttta acgtaccacg
     3361 gtatgcttta tcgcatgtgt gcgttggtgg tgatatccgg gattacgggc ggtctggctt
     3421 atccactgct gaaaatatgg atgattgact ggcaggcaaa aaatacgtat ttgctgggcg
     3481 atttggatga ccttccttta atcaataaag aagaacaacc agataaaggc ttcttagcca
     3541 ggatttcacg gggaattatg ccttctttac catttctgta agagataaaa aaggccggag
     3601 catgctccgg ccttcgtttt cattactgtg ttttgattac agcgcggcga taacagcctg
     3661 ctgttcaatc agcttcgctt tcgcttctgc atagccttcc agcttttcac gctctttcgc
     3721 gatgaccgct tccggtgcgc gggcgacaaa gccttcattc gccagcttgt tctcgatacg
     3781 gctgatttca ccttcaatct tcgccacttc tttcgccaga cgcgccagct catcttcttt
     3841 gttgatgagg ccagccatcg ggatcagcag ctctgcaccg tcgatgatct tagtaacgga
     3901 aaccggacct ttgtcatcgg caggcagcac ggtgatactt tccagacgcg ccagcgtttg
     3961 caggaagcca cggttttcat ttacgcgacg ttctgcatcc gcgctgcaac cacgcagtag
     4021 cagctccagc ggtttgcccg gcgcgatgtt catttctgca cggatgttac gtaccgcaac
     4081 gatcgcctgc ttcagccact cggtgtcggc cagtgcggct tcatcaacct gagatgcatc
     4141 gtactgcggg aacggctgca gcatgatggt gtcggcagtg ataccgcaaa gtactttcac
     4201 acgctgccag atggtttcgg tgatgaacgg aatgatcgga tgcgcgaggc gcagcagacc
     4261 ttccagtaca gtcaccagcg tatggcgagt accgcgcagt tctgcttcgg tgccaccgtt
     4321 cattaccggc ttggtcagct cgagatacca gtcacagaac tggttccagg tgaactcata
     4381 cagaatgcct gcggcgatat cgaagcggaa gctgtccagc gcttcgcggt acgctttgat
     4441 ggtctggttg aactccgcca gaatccagcg gtccgccagc gacagcgtca tttcgccgcc
     4501 gttgaagccg caatcctgac cttctgtgtt catcagcaca aagcggctgg cgttccacag
     4561 cttgttacag aagttacggt aaccttccag gcgcttcata tcccagttga tgtcacgacc
     4621 ggtagacgcc agtgccgcca gggtgaagcg cagcgcgtca gtaccgtgcg gttcaatacc
     4681 gttcgggaac tgcttctcgg tgcgcttacg gattttgtcc gccagctgtg gctgcatcat
     4741 attgccggtg cgtttttcca gcagttctgg cagcgaaata ccgtcaacca tatccagtgg
     4801 gtcaataacg ttacccttgg atttggacat cttctggcct tcgtcgtcac gaatcaggcc
     4861 ggtcatgtaa acggtgtgga acggcacctg cggtttgcca ttttcatctt tgatgaagtg
     4921 catggtcatc atgatcatgc gggcaatcca gaagaagatg atgtcgaagc cggataccat
     4981 cacgctggtt gggtggaact gacgcagggc gtcggtgttt tccggccagc ctagggtaga
     5041 gaaggtccac agtgcagaag agaaccaggt gtcgagaacg tcttcgtcct gacgcagggc
     5101 aacgtcggca ccgaggttat tttctttacg cacttcgtct tcgttgcggc caacataaac
     5161 gttacccgct tcgtcatacc atgccgggat acggtgaccc caccacaact gacgagagat
     5221 acaccagtcc tgaatatcgc gcatccagga gaagtacatg ttttcgtact gcttcggtac
     5281 gaactgaatg tcgccgttct caaccgcttc aactgccggt ttcgccagga catcggcacg
     5341 cacgtaccac tggtcggtca gcattggttc gatgaccacg ccgccacggt cgccgtaagg
     5401 aacggtcagg tcatgtggtt taatttcttc cagcaggccg agtgcgtcaa ccgcagcaac
     5461 gaccgcttta cgtgcagcaa aacgctccag tttctggaac tctgcaggga tttcgctgga
     5521 ataaacgtca gattcgttac ctttggtatc gaacacctgg gcgctttcac ggatatcgcc
     5581 gtcaaaggtc aggatgttga tcatcggcag ggcgtgacgt ttacccactt catagtcgtt
     5641 aaagtcgtgc gccggagtga ttttcacgca gccggtgcct ttttccatgt cggcgtgttc
     5701 gtcgccaacg atcggaatac gacggttaac cagcggcaga atgacatatt tgccaatcag
     5761 atctttgtaa cgcggatctt ccgggttaac ggccacgccg gtatcgccca gcagggtttc
     5821 cggacgagta gtcgcaacca ccagataatc tttaccatct gcggttttcg cgccgtcagc
     5881 cagcggatag cggatgtgcc acatcgaacc tttcgattcg cggttttcca cttccaggtc
     5941 agagatagcg gtgcgcagtt tcggatccca gtttaccagg cgtttgccac ggtaaatcag
     6001 gtcttcttta tacagacgaa cgaaaacttc tttcaccgca ttggacaggc cttcgtccat
     6061 ggtgaagcgt tcacgctccc agtccacgga gttgccgaga cggcgcatct gacgggtaat
     6121 ggtgccgccg gattccgctt tccattccca gattttgtcg atgaaagctt cgcggccgta
     6181 gtcgtgacgg gttttacctt cttctgcggc aatcttgcgc tcaacgacca tctgggtagc
     6241 gatccctgcg tggtcagtac cgacctgcca cagggtgttt ttgccctgca tgcgctgata
     6301 gcggatcatg gtatccatga tggtttgctg gaaggcgtga cccatatgca aactgccggt
     6361 gacgttcggc ggcgggatca tgatgcagaa actttcctgg ctttcatcgc cattaggctt
     6421 aaagtagccc tgcttttccc agtgctcgta aagcggctgt tcgatatctt gtgggttata
     6481 tgtcttttcc attatttcca ggttgccgta ttcaggttga aaccagccac gcggtaggct
     6541 ttatagcgtt cgcgcgccag ttgtttcaga gaatcttcat aaggaacgaa gtctaccact
     6601 tctgtgaaag cggtggcaaa atctgcaaag cttgttcgca gactaatcaa tatatcgcgc
     6661 gggctgctgc tgcgtttttg cggccaggcg atctccaccg gcgcaccgcc gcgcggtcct
     6721 tctcccgcta aattatgcgg aacaaagctt tctgccggac gcgcccacag ggcttcatcc
     6781 agccggtaag cctgcttttc atcttcacag gcgatgagca cgcgcttacc actgcgccaa
     6841 cgttctgcgg caatttcaca caccagctgc tcaacggcgc ttaagccatc gacggtggtg
     6901 tcattgtcca gaaggtagaa cgtcgcgttt ttcataaatg gggcttcttg tggtggattt
     6961 aaatattgct gcattcctca ccctaaaaga tgagcaatga atttctattg cgctcgatcg
     7021 gttccctctc ccttagggag agggttaggg tgagggtaaa acgcaaactt actcttcgcc
     7081 gttaaacccg gcgcggttta acaggaactg tgccagcaac gctaccggac gaccggttgc
     7141 gcctttcgct ttaccagaac gccaggcggt accggcgata tccaggtgcg cccagttgta
     7201 cttacgggta aagcgtgaca ggaagcaacc tgcggtaatc gccccaccag gacgaccgcc
     7261 aatgttcgcc atatcggcaa aattggactc cagttgttcc tgatactcgt cacccagcgg
     7321 taagcgccat gcgcggtcac cggattgttc agacgcggca atcagttcat gggccagcgg
     7381 attgtggttc gccatcagac cagtaatgtg atgacccagc gcgatcacgc aggcaccggt
     7441 cagcgtcgcc acgtcaatca ccgcttccgg ctcaaaacgc tcaacgtaag ttaacacgtc
     7501 gcacagtacc aggcggcctt cagcgtcggt gttcagcact tcaacggttt gaccggacat
     7561 ggtggttaac acatcgcccg gacgataggc acgcccgcca ggcatgtttt cgcagccagc
     7621 cagcacgccg ataacgttaa tcggcagttg tagctccgcg accatccgca tcacgccgta
     7681 aaccgctgcc gcaccgcaca tatcgtactt catctcatcc atgccttctg aaggcttgat
     7741 cgagataccg ccggagtcga aggttaaacc tttacccacc agcacgattg ggcgtgcatc
     7801 ttccgacgcg ttgcctttgt actcaatcac cgacatcagc gattcgttct gcgaaccctg
     7861 accgaccgcc agataggaat gcatccccag ctctttcatc tgttgttcgc cgataacgcg
     7921 ggtaatgaca ttcttgctgt agctgtcagc cagttggcgc gcctgagagg cgaggtaagc
     7981 ggcgttacag atattcggcg gcatattgcc gagatctttt gctgctttaa tcccggcggc
     8041 aatcgccaga ccgtgctgga tcgcgcgctc accgctggtc agttcacggc gggtcggcac
     8101 gttgaacacc attttacgca gcggacgacg cggttcgctc ttgttcgttt tcagctgatc
     8161 gaaactgtag agcgtctctt ttgccgtctc gacagcctga cgcactttcc agtagttgtt
     8221 acggccttta acgtgcagtt cagtcagaaa gcagaccgct tccattgagc cagtatcatt
     8281 cagcgtatta atggttttct gaataacctg cttgtactga cgctcatcca gctcacgttc
     8341 tttgccgcaa ccaataagga gaattcgctc ggaaagtaca ttcggaacat ggtgcagcaa
     8401 caatgtctgc cccggttttc cttccagttc gccccgacgt agcagggcgc tgatgtaccc
     8461 atcgctgatt ttatcgagct gttctgcaat cggagaaagg cgacgtggtt cgaagacgcc
     8521 cacgacgatg caggcactcc gctgtttctc cgggctaccg ctttttacac taaactccat
     8581 gcactacgct cctgaatctt aaagacaacg gcggtggcta cagatagaat tgcaagcttt
     8641 cgtaactcat gtccgctgtt gcgatgactt cgtgttaatc ttaacgttat tacggcattg
     8701 gcacgtcaga acaaaatctg agaggtgaat ccgttgagta taattatctt agcgacgatt
     8761 tcgacgactc aagagaataa atgacgttta agccatgaaa caagctaaaa tcctgcaaaa
     8821 gacgagtttt tacgggcgta tttaaagtga taatcataag atatctggtg cgggagacgc
     8881 tcaaaagcca gctggcgata ctcttcatct tgcttttgat cttcttctgt caaaagttag
     8941 tgaggatcct cggcgcagcg gttgacggcg atattccggc gaatctggtg ctctcccttc
     9001 tcgggttggg cgtgccggaa atggcgcagc ttatcctgcc attaagcctg ttcctcgggc
     9061 tgctgatgac gctgggcaaa ctgtataccg aaagtgaaat tacggtaatg catgcctgcg
     9121 gcctgagcaa agcggttctg gtgaaagcgg caatgatcct tgcggtattc acggcaatcg
     9181 tcgcggcggt taacgtgatg tgggcgggac cgtggtcatc gcgtcatcag gatgaagtgt
     9241 tagcagaagc gaaagcgaac cctggcatgg cggcgctggc gcaagggcaa ttccagcaag
     9301 cgactaatgg cagttcggtg ctgttcatcg aaagcgttga cggcagcgat ttcaaagatg
     9361 tgttcctcgc gcaaattcga ccaaaaggta atgcacgtcc ttctgtggtg gtggccgatt
     9421 ccggacattt aacccagctg cgcgacggct cccaggtcgt cactctcaac cagggaacgc
     9481 gcttcgaagg cactgcactg ttacgtgatt tccgcattac ggacttccag gattatcagg
     9541 cgatcattgg tcaccaggcg gtggcgctcg acccgaacga taccgaccag atggacatgc
     9601 gtacattgtg gaacactgac accgatcgtg cccgcgcaga actgaactgg cgtatcacgt
     9661 tggtattcac cgtgtttatg atggcactta tggtcgtacc gctgagcgtg gttaacccac
     9721 gtcagggacg cgtactgtcg atgctgccag ccatgctgct gtatctactt ttcttcctga
     9781 tccagacctc cctgaaatcg aacggcggta aaggtaagct ggacccgacg ctgtggatgt
     9841 ggaccgttaa cctgatttat ctggctttgg cgattgttct caacctttgg gacaccgtgc
     9901 cggtccgccg cctgcgcgcc agtttttcgc gtaaaggagc ggtgtgatgc aaccttttgg
     9961 cgtacttgac cgctatatcg gtaaaactat tttcaccacc atcatgatga cgctgttcat
    10021 gctggtgtcg ctgtcgggca ttatcaaatt tgtcgatcag ctgaaaaaag ccgggcaggg
    10081 gagttacgac gcgttaggcg caggaatgta taccttgctg agcgtgccga aagatgtgca
    10141 aatcttcttc ccgatggcgg ctctgcttgg ggcgttgctt ggtcttggga tgctggcgca
    10201 gcgcagcgaa ctggtggtga tgcaggcttc tggttttacc cgtatgcagg tggcgctgtc
    10261 ggtgatgaaa accgccattc cgctggtctt gctgacgatg gcgattggcg aatgggtcgc
    10321 gccgcagggc gaacagatgg cgcgtaacta ccgtgcgcag gcgatgtacg gcggctcgtt
    10381 gctctctacc cagcaaggct tatgggcgaa agatggcaac aacttcgtct acattgagcg
    10441 ggttaaaggt gacgaagagt taggtggcat cagcatttat gcctttaacg agaatcgtcg
    10501 tctgcaatcc gtacgctatg ccgctaccgc gaagtttgac ccggaacata aagtctggcg
    10561 tctgtcgcag gttgatgaat ctgatttgac caatccgaaa cagattaccg gttcgcagac
    10621 ggtgagcggc acctggaaaa ccaacctcac gccagacaaa ctgggcgtgg tggcgctgga
    10681 cccggatgca ctctcaatca gcggtttgca caactacgtg aagtatctga agtcgagtgg
    10741 tcaggatgcc ggacgttatc agctcaacat gtggagcaaa atcttccagc cgctatccgt
    10801 ggcggtgatg atgctgatgg cgctgtcgtt catctttggc ccactgcgta gcgtaccgat
    10861 gggcgtgcgt gtggtcaccg gtatcagctt cggttttgtc ttctacgtac tggaccagat
    10921 cttcggcccg ctgacgttgg tttacggcat cccgccgatt atcggcgcac tgttgccaag
    10981 cgccagcttc ttcttaatca gcctgtggct gttaatgaga aaatcgtaac caagcaaggg
    11041 agcgaaagct ccctttcaca ttgttgataa agtcaaatac gagttcgatc ggtcccctcg
    11101 cccctttggg gagagggtta gggtgagggg gacagatcgc gcctgactta ccttcttctc
    11161 ccccccagca aactccccaa catcccgcgt acaatctgat tcgtcacctg gcgagcggcg
    11221 cttttcgcca ttgtttgcac cacaccatct ttcttcccgc cgcgtggtcc ggtagtgcca
    11281 aacaaaatat ccttcaatcc accaagaata ccgtcatcca ccgccacctc tttccctttc
    11341 gtggggggat tcttttgctg ctcggtactg gcctgaacgc ctttttgcag catctcatag
    11401 gcggactcgc ggtccacgtc atcctcgtac ttgccataca ccggggagtg attaatcaag
    11461 ccattacgct catcttccgt cactggcccc attcgcgaac aaggcgcgat caccatcgct
    11521 cgttccacca ctgaaggact tccttttgca tcgagaaacg agattaacgc ctcgccggtc
    11581 cccagttcct ggattgcctt ttcggtatca aatgccggat tggcccgcat ggtttgcgcc
    11641 gcagctttta ccgctttctg atctttgggc gtaaaagccc gcaaagcgtg ctgaacgcga
    11701 ttaccgagct ggccgagcac attatccgga atatcagacg ggttttgcga aacgaaccag
    11761 acgcccacgc cttttgagcg aataagcctt atcacctgct caatcttatc cagcagtacc
    11821 tgcggggcat cgttaaacag cagatgtgct tcgtcgaaga aaaacaccag ttttggtttc
    11881 tccagatcgc ccgcttccgg caattgttca tacaactctg aaagcatcca cagcaggctg
    11941 gcggcgtaca gtttcggcat ctgataaagc ttctcggcgc tgaggatatt gataacgcct
    12001 ttaccgttgg tatcggtgcg catccagtct ttgatatcca gcatcggctc accaaagaag
    12061 tgtgctgcgc cttgctgttc cagcgacaat agcccgcgct ggatggcacc aaccgatgcg
    12121 ctactgatat ttccgtactg attctggaag gatttggcgt tatcgccgat gtactgggta
    12181 atcgcccgca aatctttaaa gtcgagcagt aacagcccct gatcatcagc aatgcggaag
    12241 atgatattca gcacgccaga ttgcacatcg ttgagattca acagccgcgc cagcaacagc
    12301 ggccccagat ccgaaaccgt cgcccgtacc gaatggcctt tctcgccaaa gatatcccac
    12361 accaccaccg gattggcatg cggttgccag tcattgacgc cgatattttt aagccttgtg
    12421 agcagttttt ccgacgccgt tcctgcctgc gcgataccgg tcagatcgcc tttcacatca
    12481 gccataaaca ccggcacgcc gatttccgac aatgactctg ccagtttttg cagcgtaacg
    12541 gttttacccg tccccgttgc gccagtaatc agcccgtgac ggttagccat tcccggcagt
    12601 aaaaacagtt ctgtgtctgg cgtgcgggca attaacaggg gttcgctcat gagatttcct
    12661 ccagtttcct gcctggagta taggcaacgg ctgcgcactc ttcaccgcta aattcagcag
    12721 attgcgagat gccttccaaa gagattttag tgccgggcga caggcacccg gcaaagggat
    12781 cagaagtcgg ctttcagaac cacgcggtaa cgcgccttac cgtcgcgcac atgcttgatg
    12841 gcgtcgttaa ttttcgacat cgggaacaat tcggtggtcg gtgcaacctt gctgcgggcg
    12901 gcaaagcgca tcagcttacg cagctcataa ggcgtgccgg tagcagaacc agagatgctg
    12961 cggtcgcccg caattaacgt aaaggccgga acagacagcg gcgtgagaac cgcaccgacc
    13021 gtatggaaat taccgccata ggtcagcgcc tcaaaataag gctgccagtc gaggctgacg
    13081 ttgacggtgt tgataatgag atcaaactgc cccgccagtg ctttgagtgc ctgcggatcg
    13141 cggctattca ccactttatc ggcacccatc gccagtactt cctgctcttt cgccggattc
    13201 gaactaaagg ctgtcacctc gcatcccatt gcgtgcagaa gttttatagc gatatgcccc
    13261 agcccgccaa taccaattac cccaacgcgg ctggtggctg tgatatggtg catcaacagt
    13321 ggtttaaaga ccgtgatacc accacacagc agcggcccgg cggactcgat atcaatattt
    13381 tctggcagtg gaatcaccca ttgccagtct gcacgcaact tctcggcaaa accgccgcga
    13441 ttcataatcg tcggcaccgc gccttgctcg cagttgatct gattaccgct aatacaggcg
    13501 tcgcagtgac cacagctacg cgccgtccag ccaattccga cacgctgacc gacctgcaaa
    13561 cctttatcct gcgcggcgct cccgagcgcc accacgcgcc caattacctc atgcccggca
    13621 accagcggat attgtgaaaa tccccattcg ttatcgatca ttgacagatc ggaatgacag
    13681 atcccgcagt aatccacctg cacttcaaca tcttgtggct tcagctcacc gggatcgtac
    13741 tcataaactt ccagttcgcc gcccgcttct tttgcggcat agctttttat catcgacatt
    13801 ttttggtcct tctctggtgt tgtctgggaa gtgtagagca tggcagggcg gggtgcctgg
    13861 agtgtgacaa aggttacaca tcgctgtatg caatgctgaa aatttcagca cttagcgagg
    13921 tgcgagcaag ctggcgcttg catggtggcg tgcgacaggt ataatccaca acgttttccg
    13981 catacctctt cagtgccgaa gtggcgaaat cggtagacgc agttgattca aaatcaaccg
    14041 tagaaatacg tgccggttcg agtccggcct tcggcaccat cggaacatca atagacgtca
    14101 acggacgtct ttttttgtgc ctgaaatcca gtatttaccc atcattcccg ctatattaac
    14161 tctctcaagg tcaaccgact tcaacgtgca tctaccaaca gatgttggta cagatgatgg
    14221 tatttccggt tcgataatgc ttgtaccaac agggagggga taagcatggc tctaacagat
    14281 atcaaagtca gaacagccaa gccaacggat aagcaatata agctgaccga tggcaacggt
    14341 atgcaccttc ttgttcatcc aaatggctct aagtactggc gtctgcagta ccgatttggc
    14401 ggaaagcaaa agatgctggc tctgggtgtt taccctgacg tatctcttgc agatgccaga
    14461 gctcgtcgcg atgacgctcg taagctttta gcaaacggca tcgatccggg agataaaaag
    14521 aaaaatgata agattgagca ggaggaagca cgtacttttg agcagcttgc tattgagtgg
    14581 catgccacaa ataaaaagtg gtcggaagaa catagtcgac gagtgcttaa aagcctggag
    14641 gacaatctct tccctgccat aggtaagcgg aatatcgctg atcttaaaac tagagacctg
    14701 ctggccccaa tcaaagcggt agaactgtca ggacggcttg aggttgcttc acgattacaa
    14761 cagcgaacca cggcaattat gcgctatgcc gtacagagtg gtttactgga ctataacccc
    14821 gcgcaggaga tggttggcgc tgttgcttca aataacaggc agcatcgacc tgctcttcag
    14881 ttgaagctaa tcccagaact attacggcgc atagatagtt ataccggtag acctcttact
    14941 agacttgccg tcgaactcac gctcctggta ttcattcgtt caagtgaact acgctttgct
    15001 cgttggtctg agatcgattt cgaaacatca atgtggacaa tacctgcaga gcgcgaagct
    15061 attgaaggag taaagcattc acagcgtggt tcaaaaatgc gtacgcctca tctggtgccg
    15121 ctttctcatc aagctttagc aattcttaaa gatgtttaca aacttagtgg tgatcgcgat
    15181 tttgttttca tcggcgatca tgaccatcgc aaaccgatga gtgaaaacac ggtgaacaag
    15241 gcgctgcgcg ttatgggtta tgacacgaag gtagaggttt gtgggcacgg tttcaggaca
    15301 atggcctgta gttcattgat cgagtctgga ttgtggtcga gggatgcagt ggaaaggcag
    15361 atgagccata tggaacgtag ctcagtacgc gcagcttaca ttcataaggc cgaacatctt
    15421 gatgaacggc gactgatgct gcagtggtgg gctgattttt tggatgcgaa tagggaaagc
    15481 gaagttagcc cttatgactt tggcaagtag tctaattcct gatgcttgct atgttcgaat
    15541 ttgaaaagcc ggcttgaact ataacccata cttttttata actgtattat tctggcgact
    15601 cagaggtgca ctatgaaaac tttcgatact caacttgcga tgagcattgt atcgcactca
    15661 acgggaggtt ttagttatga cacactcgct agggcattga tttctaaaga taaaaacgaa
    15721 aattctgacg tccttcttga gtcatgtcga aatttttgtc tctctcttga ggaaaaaggt
    15781 attctgaggc gatgccaaag atgttctcat tctcaagatg agtatttcga gtatattcct
    15841 cactaacatc agtccctgaa aattttttca gggactgatt actccgttct tgtcatcccc
    15901 ttttttctcc tgtagcttgt gtaacacgat tctggggggc gattgtgagc aacatcaact
    15961 taataaccac atgtacaaac ggcaaacact acggtagtag ggatatattg agcctgagtc
    16021 aattttcatc aggccgaacc ccttcaaatg tgctcgtgaa ttcttggtgc aatgctctga
    16081 atgaagctat ttctacatct gcaactgtat gtgcagagga tctttataaa ggtgggcact
    16141 gggcgacggc taaagccatc ttagatgatt atcctattga tttatggatt ctttctgctg
    16201 gtttaggact tcttcaccac aaagacaatg ttgtacctta caaagcgacc tttgctgttg
    16261 gatatgacga atcaatacca ttgtattctc aagaatatgt agggaaaagt tttcatagaa
    16321 catggtggaa agaaataacc aatagatcta tttttaaatc taagcatcct acatctattg
    16381 tagaattaat gaaaaaaaga aaaagagact attatattat atgtggctct ccagattata
    16441 ttaatgcaat tgaactagat atcattaatg gtcttgagta tctagttgat gcaaaaaaac
    16501 aactgcttat cataacttcc aaaaaaatca atggtaggtt aactacatac cttttcaaaa
    16561 ccaaccaaaa tatggctcag tggctgagat gcaatatgct catgcttaat atcagtgtgg
    16621 caaaatacat tgtgaaggaa tttacgtcaa aacaactcaa tgatttaaat gagttatctc
    16681 aaaagttaaa agaagaactc aaggagttac ctgagcgtga agtgaaaaaa ggaattcgtc
    16741 gtagtccgga ggaagttaag tctttcattt taaagattat ggaaaaaaat cctggtatca
    16801 gtgcaacaca cgcgttgcgt gaatttcgtg actcggggaa ttcatttgag gaaaaacgtt
    16861 ttcgggctga gtttatggcc ctgagagagg ctaaacctta atgctacttg cctgggtttc
    16921 atggtagaat cccccgtttt ttgaggcttt gcagtggcgt cacaggagag taagttgtcg
    16981 aagctaaagt attttttccc tgatagtcag gattttatcg atcccagttt cgacttcttt
    17041 cgcgaaacgc gtaacgaaca tcgcgtgcgt caacgtgacg atcagtatcc gcatgaggtt
    17101 tttcctcatc cttacgatgg tatgctggtt tctaaagctg tagttgatgg tcttggcggc
    17161 ggcgagagca aatatactcg tgcccagcgt ttgcgttact tccgtaatgg aatgaagcac
    17221 tttttccgtc tgccggaaaa tatggaaact atgggtgatt gcggagcctt cacctatgtt
    17281 aatcaggacg tcccgccata ccgtgtggaa gaggtgattg agttttacga aacttcccgc
    17341 tttaaccatg gcgtttctct cgatcatatc gtttttggct atgaaaaacc tggtgaggta
    17401 tttagtggcg aagtattgac tgagtgtcgc cgacgccagg atatcacgct gaccttggct
    17461 caagagtttt taaataagtc tcaaaagtcc tgtttcaccc cctttggcgt tgcacatgga
    17521 tggagcaaaa cctcctatcg tcagtctgta gaggccctgc tggccatggg atacaaaaat
    17581 atcactatgg gggggatggt gccgttgaaa acggcgcaga tcctagagac tctcgaagag
    17641 attaagccgt tgctgaaaag tgatacgcgt gttcacttgc ttggtattgc ccgtccagag
    17701 agtttctccg actttatcaa gtttggtgtg accagtattg actcgactac accgcttcag
    17761 caggccttca aagatcgaaa aaacaactat catacgcttg atggcccagc ctataccgct
    17821 gtgcgtgtcc ctcagtttga tgcgaatcca agtttgagtc gcaaaattaa atctggcgtg
    17881 attgatcagg atattgcacg gcatctggaa aaggatgcca tgaatgccct gttcgaatac
    17941 gataaagggg ctttgtccct tgagaaggct cttgatgcag tcctggctta tgaacgtctg
    18001 cattcgggtg aaaaagaggc cgacaaaatt cgtgcggatt atgaacgtac gctaggagaa
    18061 cgaccctgga aacaatgcca gtgcaatatt tgccaggcca tcggtatcaa tgtcatcatt
    18121 ttccgcggag cagaacgtaa ccgtcgccgc ggtttccata atattcaggt gttgtacaac
    18181 cgtttacagc acacattgtc attacgctca gaggaactgt catgagtgaa tatcgtgttc
    18241 ctgccctgcg aatccgacag ggtgaggaga ggcaactcta tagccttgcg atagaaggta
    18301 aacagatcag caaaattgca gcgatttcgc gtattcgtcg cggcgaagag agtttggttg
    18361 gttatcagcg tccagaagta cgtaaccata ttcgcgaaat tcagcgctac attgagagcg
    18421 caaatccgat gataccaaac ccggtgatta tcgcttttga taagcgtgtg cgttttgaac
    18481 cgcttacaga taatgacgac atgggacatc tggtaatacc tttctccgaa gataaggatt
    18541 ttgagaagcc aggttttatt gtggatggtc aacaacgtac tgctgctttg cgggatgcag
    18601 aaatcaattc gtttatgatg ccggtctccg cttttatcgc taatgacgcg gaagaacagc
    18661 gtgaacagtt catgctggtt aactccacaa aaccgttgcc caaaacgttg ttatacgaac
    18721 tggcaccgca tacccacggt cgtttgccat ccgatctgca aatgcgtaag tttccttcac
    18781 tgctgactca acgactgaac ttcggcgaag gttcacttgc agggcgtatc aaaacggcga
    18841 ccaatcctga tggtgttatt gctgacaact cgatgatcaa aatgatcgat gccagtcttc
    18901 gcgagggagc attgtatcgc ttccgtgacc cggcgacagg gttgggggat gaagagaaga
    18961 tggtgaaact gctgaataac ttctggtcgg ctgtggaaac tgtgtttact gatgactggg
    19021 ataaaaagcc tcgctattcc cgcttgttac atggcgttgg tattcttgca cttggcagct
    19081 tgatggatga aatcgatcag gtccatcagg actataaagg tgaacctggc tggactgaga
    19141 tcccttccta cactcgtttt gtcgaagagc taaaccgaat aaagccgctt tgtgcatgga
    19201 gcagtggcgt atggaacttc ggcacagata ttgatggtca gcctatcgtc cgtaaatgga
    19261 acgaactgca aaatctttca aaagatatat ctttggttac tgactatttg gtaactggct
    19321 atatgaaaac tgtcaatgca gatattaaat aagggtgaca acccaattgg cattagaagt
    19381 atcgagtgct gattatcgac gtggggggct gcagatatgg tgcagaagtt caaattctgt
    19441 gcaagcgcca gacagccccc atgctttcat tcttatcatc aaacgctgac cttgttacga
    19501 tatatgttcg taactcatga aaaatcaaga gttttttgtt gaaattcagt cgtttcacga
    19561 gttttgaaag ccggtaaata agtcataggc cgcaccggat tacccaccaa tcccccctaa
    19621 aagtcatagt agactagtaa ccgtgatccc cctccttgac aatcatcttt acgggttgca
    19681 gtgtatgaca ttgtccttgt attcagtgat attatacgta taaaaaatca ccattaatca
    19741 aaggctttta aaatcactgt tacgcacagc aaatttatgg atggatatat gccccttgaa
    19801 gttggcgacc tcgttgactc gccttatgga atcggcaaac ttattcgcat taacaataat
    19861 gatgctctaa ttaagtattt cgattctccc gtaaattatg aaggcataga gcaaactgtc
    19921 gaagtaaaac acctaaaaaa agccgaactc cacgcggaaa gtctggttta tcgctacgac
    19981 tacgaggttg cacactggca gattgcacgt gttgttggtg ccgtgcctgg aggggtacgt
    20041 ttgcattttc ctaacaagca gcaggaagac gtcagcttaa atgatatctt tgtgcgctgg
    20101 gatcgcccta tcagcgatgc tgcggccctt ctggccgagc gcatcaccta tacccccttc
    20161 tggcaagatg cccgtctagc ttatcagcat cagatgctga aacaacgcaa cgcttgtgct
    20221 gggattacct cttcgctgtc gtccagtatt gaactcgaac cttaccagtt ggccgtcgcg
    20281 cgtcgggtgt tatctgaccc tattcaacgc tatctgttgg ctgatgaagt cggcctcggg
    20341 aaaaccatcg aagctgggct gatcgtgcgt caacacgtca cggaaaatcc gctcgcccac
    20401 tgcgtggtga ttgtggcgcc tggagttctg caccggcagt ggcagcagga actttctgca
    20461 cgttttcacc tggaggaact gcttaataag tcgatccata tcattgatat ggccgccttc
    20521 agcacagcgg accccctgtc gttccgcccc gatatgctgg tgattgacga agctcatcag
    20581 gtcggtgcct gggcatggga tgcagagtac gagcagcgct acgctagtgt gaactacttg
    20641 gcgcaacaaa gtgaaaaatt gctattacta tcagcgacac ccgtaactgg caatgaaaag
    20701 aacttcctgg ccatgctgca tctgctggat gctaaaaatt acccactgac tgacactggc
    20761 ttacagaaat ttcagcagcg tatccagcag cgcgaagaaa taggttcact gttctacgcg
    20821 tttaactgtg ataacgacaa catgacgctg gaagagaacc tggaagcgtt gactaggatg
    20881 tttcctgagg atgaagtact aaacgggatg gcttcacagt tgcttcccca tttagatatg
    20941 atgcaaccgc tgcggagtga ggcgcgtgat ctgctgatca ataaaatccg ccactacatt
    21001 ggtaaccgtt accgtctgca ccaccgcctg ctacgcaacc gccgtagcaa ccccgccatt
    21061 agtgtcctga tgcctggctt gtccggccta tcactaaaaa aatatgccac gacgaacgcc
    21121 agcaacgaca tgatgatcgc ctggcgcgaa tcggcaataa tgatgcaccc acaagacccc
    21181 acgttggcgg caatttatca gttactgttt gaagccgcta tcgctacgcc ggaactactg
    21241 gcccggcttg caaactatcg cttacagcag gagtcacttg gtgccgcgca gggctttctc
    21301 tatagtgatt cacagctcgc gctgttgcag catccgctgt ttgatggcga gaaggagatc
    21361 ctgcttgagc tgatagaccg ctgcaaaatg gagtgtgaca gcaagcgcca gcgcttaatt
    21421 gatgtattgg aaaaacttct ggatagtaac gtcagagaac tcccttatat tgtggtgttc
    21481 tgtgaccagc catgggtagc cgaccaggta tttaccgacc tgatgatacc atttattggc
    21541 aaaatccagc ggcataaccc cgacaaagaa gtgcgctttg gcaagggaag caaatgccag
    21601 atcttagtgt gcgatcgccg ggctgaagaa ggtgtcaatc tgcacggcgg gcgcaagatt
    21661 gcgctgcatt acgacttgtt gctgtcgcct aaccgtatgg aacagcgtct tggccgcttg
    21721 aatcgctatt gcgcgactga gagtgcgact actatcctca gcatggcgct ggtgagtgaa
    21781 gcagagggtc tgcaaaatca gtggctgact attctcaatg aagtgttcga tgtcttcgaa
    21841 gagtcgattg caagcctaca atacttgatt gatgaggaat tacagcggct tatttccaat
    21901 ctttacccgc tgggtgaaga gagcatgatg caactcagtg cgcgtctggc tggtgaggat
    21961 ggccgcctta cggtggaaaa gcagcgcatc gaaatgcagg aagtcctgct gcaaattgat
    22021 ggcgatatca tcgaagccag agagtatgcc gatgcactgg cggaaataga tgagaatgaa
    22081 gaggaattcc gccgctgcgc tcgtaatctg ataggtaatg tactgaaatt cgattttatt
    22141 ccagatgaga aagatccatc gatattccgc tatcgcttca accctggtga tagagggacg
    22201 ggcaccttag tcagcccaca gcgcctacaa aaacactgcc tgctgggctt tgatctcaac
    22261 caatctagcg gaagttcgcc catgacttgg ccacttagct actaccgggc tgatacctct
    22321 cagggtaaaa tccgcccggc tcgcttgggt gaacctttcc atgatgccat cctacaaatg
    22381 ctggattttg aagaacgcgg tgttgctagc gcgattatcc gtatcacacc aggcatgggc
    22441 gatgaaaata tttttttccg ctacgatttt ctgattgaag ctgatgtcca tacaccagca
    22501 ctgcaacgac tggcggacca tctgatgcct cctgaaacca ttaccttgtg gctggaccag
    22561 gccggccagc aggtcaccaa cgcagaggtg ttgatgatac ttaatgaaga ctataaaccg
    22621 aaggataagg gcggacgcca cctaaatctc aatgaggaac gctgggcgca gatctcccat
    22681 tgcatcagcg ctaaagattg gcgcacctgg tgtaacgata gctatagcat agcccagcgc
    22741 ctggcggtgg aacagttcgc cacacaacag gctctcagcc tgagccgact ggaacattat
    22801 ctaagcagcg ccgcgctgca tcatgctaac cggcaggaga cagggcagat gctgcgccag
    22861 ggtattgcac agccgaaaat gacctgcctg gcgacgcgtg ccttaatcat tgcatcagaa
    22921 gctatattgg atgaggactc ataaaccgga tgaatttggc gcaactgatt gataatgatt
    22981 ttatttcatc aaggttgagt gagtgcctgc tgccagaggc gactgccgga cacctcgacc
    23041 gccgggcgtt gatccgtgac tggctgtacc gggaaaaaca tgcccaccac aacgataacg
    23101 agctgaccgt ccctgatgat agccaacctg gttggccaag acaggaggag tgggaagcca
    23161 gcggtattaa ctgtcaacga ctaggtcaga cctttctgct cagcgctggc cctgcttggt
    23221 gtccgcagtg gctggagcag ggaaacggca tgccttttct cgccagcgat cagcaccaat
    23281 tctgccgcca ggacactcga gtaccgatgg actatgtcct gcgccgttac ttcaagaagg
    23341 acaactatat cagctatcag gcaccgggcc agcgcgatgc ggtacgccgg gctctgttcg
    23401 ccccaccggg cagtacgctg ttggtcaatt tgcccacagg caccgggaaa acactggtag
    23461 cgcaagcatt agctctgaca gcctcagctc ccggggcact ttgtgtactg atcgccccga
    23521 ccacggcgct ggtgttggag ctggctagcc gtttccggaa gttactgatt gaagcaggga
    23581 acaaagcaga atctgtcacc ggctggtata gcggcttacc ggcgcaggat aaatatgata
    23641 cgtacatgcg tatccgtgtg ggtgagcagc ggctgatcgt cacctctcct gaagcgacct
    23701 gcagttccct gcagttcgcc ttatttgaag cagcaaaaca gggagggctg cgccatgtta
    23761 ttgttgatga agcgcatatt gtcgccagct gggggaacga ctttcgcccg catttccagc
    23821 aacttggtgg cttaattcgt gggctgaagc gcgtcagccg tgaagcgaat cttacaccgt
    23881 gctcaacact gctgatgagt gcgactatca ccgaagccag ccgtaaaatc ctgcgcgata
    23941 ccttcgatca ggagatgagc gaactccatg cctgccatct gcgcccggaa cctgcttact
    24001 ggtgttatcg ggcaaaagac cacagcgaca aacgggataa ggtgctgcaa tcgctggctc
    24061 atgcgccacg cccgctgatc ctctacgtga cgcgaccgaa agaagctgaa gagtgggcgc
    24121 aacagttgcg tttgcagggg tatcagcgtt tggcaacatt taccggtgat acaccgagtg
    24181 aagtacgccg aagcctgctg gaaaaatggg ataaaaacca gctggatatc atgattgcga
    24241 cctcagcatt tggcgtaggt atggataaaa acgacgtgcg cacagtgatc catgccacag
    24301 tgccggaaaa catggaccgc ttctaccagg aagtgggacg tggtggacgc gatggctatg
    24361 cctgcacctc actgctaatt tacaatcatc aggatgtcaa acaggcagaa catctgtcct
    24421 cacaaaagtt gattggtctt gagttgggcc tggaacgctg gaaaaagtta tggggcagtg
    24481 ccgaatcggt gggcgacaac ctgtatcgta ttgatataga gaggatccat agcggcctta
    24541 atttccgcag caaacaaaat aaattatgga acttgcgtac cgtactgatg atggtgcgtg
    24601 caggagtgct ggaactggat gccgatctac aaaacccgcc agacaaaaaa gactttgccg
    24661 atgttaatcg ctacgaagcg gcaaaagagc aaatcatccg acagaacgga aattcactgg
    24721 tagtacgcat cctgcgtagt caccacaccg gagaagaatt gtggaaaaca cgtattatcc
    24781 cttcccgtga agagacgctg caagctgcaa gggctaacca taatgcatta atcaactggc
    24841 tgcatacgcc actgagcaga ccgctgtgtg aggcacttgt agatctctac tccatggcgg
    24901 aatttattcc gggtcacgcc tgtggtggtt gccctgcctg tcgcgataat caggcgctta
    24961 ctagtaatta tccgagccca ccgacgctgt tgcttaaccc gccagcgtcg gtggcacagc
    25021 aggcgttaag ccgctggcag gcgctcagcg gcctatctta ctccagttgc tttatcacct
    25081 atccgccagt taacgacgat gagtgggaac gtgagaactg gtatcaccgc atcgcgacgc
    25141 tactggtgca actgtatcat agaggcattg tccagcgcgt ctgggcagat cctgccttgt
    25201 tgcatagtat atttagcgcc attccgcgcg gtgagcgcct gatcttgttg agtgacgaat
    25261 tgcctgcacg tgatgagcca gcgccggata acgatgtaat ggccgagttg gcagagttgg
    25321 tacttcccac tcaagagtcc tgcgccaatg gttacctgcc gcctttcaac ccagcaccat
    25381 tacaactgac gctgatcccg catgatatgc ccgatcctca ccatcctggc ggggcgcatt
    25441 acgttcttct cgataatcac aattggatca ccatcgaaca acttcaaagg aaactagaca
    25501 gtgtcgatta tcaataatgc ccatggtggc agccagtatg ccgtgctggc cgcattttat
    25561 caggtgctga aagccagccc gaagaaccaa ctaccgcgtg aaaagctgat ggcgctctgt
    25621 gcaccggaaa cattgcaaac cggcgactca caaaatgcac gtggtaaagc gtctcaggaa
    25681 ttaagagcat ggctggactt ggggttactg acggaagaaa aagtagaaga gacaacacaa
    25741 atcagtttca acgcgaacta tttcagcaca aaaagtatgc cgctgcgcca ggctgcgcgc
    25801 cgctgtttac aggcacagga aaataacagt gacctgagct cacgcgatga acgcgccgtc
    25861 gaccttaccc ttttggctgc attgctgctg gcgctggatg tctatcagaa cgcagaaatc
    25921 aaatcgggca accttgaagt gctggtgaac acgtttatac ctgattttcg tatcaacagt
    25981 aatgaaattg ctgtcgttcc ggggtatctc agttggctgg gctttgctca acaactcact
    26041 cgcgacagtt acgccattga ccccaccgat tctattcgtg aagagttacc gttccttatg
    26101 cgtgttggcg aacaactttc catcggcgaa gtgctgcaac gtcttaatcg ggcgctcccg
    26161 gtgctagatg gtggcagtta tcgccagcag gtcgaggagc gtatcagtct gtatggctgg
    26221 cactctctta gccctaaccg cctttccacc tcgctgtcgc gtgcgctgct gcggcttcag
    26281 ctttccggag taatcaacat gaaggctgag tctgacgcca gcggcgcaat gcagttaacc
    26341 ggccggaacg gcagcgtgct gcgtaccgta acccatctga ccttgaatga tgtgggagtc
    26401 cactaatgcc acaactacag agctactggc ccagcgagca gaacgttact gcctgcatcc
    26461 taaccgaggc tgagtcactg gctgacagcc agttgctggc ggtgcatgaa ccgatgcgtc
    26521 tcgaccgcat tgagtttcaa agtggtaagg tcacgcaggt gggagagggg gcgctgctgg
    26581 actttctgct tgaacataac cgcccgctgc cgctaattgg cgcttctggc gtggggaaat
    26641 cccacttggt acgctgggtt catgctcagc tcaaacgtcg tgaagatcat gcgaaatacc
    26701 acattatccg catccccaaa aatgcttctc tgccacgtgt acttgccgcc attctggaag
    26761 gtcttgacgg cgaggagtat cagagtattc gtgaaaaggt taacggagtt ggaaagcaat
    26821 taatccccga aaacgttgcc gaacatattg ctcttaaatt gcgtcaagcg ctaaacgcag
    26881 catatacaga tgccattgaa gagcttcgcc aagaaaaaat gggaaaaatt cagctggaag
    26941 aaagcagaaa aaagcagctg gaagaaattc aacaacacgc agcatcatcc cgactgcccg
    27001 cgctgttttt tgacagcgtg atgacgcagt atttcactgc tcctggcgcc tgtcttcaca
    27061 atatagcgca gcgcttctgc cagggagcta acgacgacag tattgccaac ttgcggtatg
    27121 aaatgtctgc ggaagacttc accttcaaag gactggactt caggcaggtt tcaccaacag
    27181 tgcttcccta cctgcgcaac caacagttgc ttaccagtga tgaaaaaaag caagcagcgg
    27241 ctcgcgttgt caacgtggtg cgcagccagg cgttagaaga caccttcgtc gaactattta
    27301 gctttaaccg ggccaatttc caggatctga tgcgtatgat ccgccgacag ttgctggcag
    27361 aaggtcgcga cctgatcctt cttgtggaag atcttaccgc cacctcggcg attgaagatg
    27421 tcctgatcgc ctgtctgata gaggaagatg agtacgacgg caaaaaggtt ttatgtactc
    27481 taagatcgat tattgcggtt actgagggtc acgatagctt taagcgccta cgtaatacac
    27541 taggcacccg cgcccgctac gaatgggtga tccagcagca tgccagcgaa aacgatgtcg
    27601 cgctgaaaaa acgcgttatt gacttttgcg gccgctatct caacgctgcc cgtcacggcg
    27661 ctgctgctct ggaaacttat cacctccagc aagacgcgca gtgttatcag gagattcctg
    27721 tatggcagga tcaggaggtg ctggagaacg aatctgctgc gccagcgctg gccagttttg
    27781 gcagcagtag cattggtcat ccgctgtttc ccttcaaccc agcagccatt gggcagttgg
    27841 tagaacgaca ctgcaaagtt aaagaacaag gcctagtgtt tatcccgcgt aatattttgc
    27901 atgaaatttt gcgcgagcca ctgaaaaagt atcgccagaa ttatctggac gggcagttcc
    27961 cgccatcaca atttgctgag attgtatgta acccggcatt acagcagagg gtacgcctgg
    28021 aaggcctcag ccagcctgag cggattaatt cactgctggc tgtttggggg gggaatagta
    28081 caaacctggc aggtcttacg cccgccatct gtaaagagtt tggtctgccg gatgcggcag
    28141 tactgttggg gggcggtgga acgccaccgg aaccagcacc ggaaccagta ccggaacctg
    28201 caccagtacc ggaatcagta ccggcaccac catcaccact tgatgaatgg aaactcgtgc
    28261 tggataaatg gcaccagagc aagacgctcg agcaaaccag agcccgcgac attcgtaagt
    28321 ggctcctgga agcgctgaat gcccgtatcg actggtcaac tgagcttatc cagacctctc
    28381 tcatggccag ctcagcaaaa actcagggta ggatccactt gcccggcgtg cttgtaggga
    28441 atcagtcaca tccgctggtg aagcttgaag atactcctga gttgcgtagc gcctgtctgg
    28501 cgatggcgca cttcaaccat tatcagagtt gggattatga cggcagtgag atcgactacg
    28561 ccgcctttca tagtttcatg gattgcattg agcacgaagt aaaactcaag gtgttgcagg
    28621 aagaacgcga ggagctttct agtatcatca acgatctgca gttaagtggt gcattccttg
    28681 ggttggatgg attatcagca tcccacccgg ccacgctggt gcaggcactg ttaacggaac
    28741 cgcaagtcgc aaacagcaac catccactgc tggatgagtt cttaacccaa gcgcgcgaca
    28801 ccgtgattga tgagcatctg aaacgtcgcg agcgtttaat cgcgcttacg gcggcgaaaa
    28861 aaccgggtgc tcagccgtat gctattaacg gcttaatgct ggtggaggcg tctaagaaac
    28921 aatcgccaga cgctggcaaa caaccgggca agatccatga agtcaaaagt aagctcggtg
    28981 gctaccgcac cctgctattg aagacccaag agagcatcac tgaacgcctg agcgacggca
    29041 acagcgccag cctggcagcg gtgaacctct gcgacaccat gaaggagata gtcgaactgg
    29101 cgaatagcgc ggacgtcgcc cgtccaatgg gtgaaactgt accggtactg ctgaagttgc
    29161 tgaagagctg gaaagatcca gagctgaaaa aactgatcag acaactggaa aaactgacgt
    29221 tgccagccga taactcgcac atgttgatga gagaaatcct tgccatcaat ggcgagcagt
    29281 ttgaggcgtt tcgacagtta ttagagaatt ggggcaagtt cgaagatgcc accgacaagt
    29341 atgtgcaaca gcgtataagc gataagggcg gagaccaagt tgaggatgca gaaaaccaag
    29401 ttacagatac cctggcaaac ctggctgaac agttaacact gctggaggcg gacttatcat
    29461 gagtgaagta aacatcaatg acgtggtaac tcccgtagcc agcgtaccgg aagaacgcgg
    29521 tatttttgca cggcgcagcg gtgattttaa agcacgcctt caacgctacc aacaaatgga
    29581 gaaagctcag gatatccgcg ataacctgct gcggatagca aaaaaaatcg atactccgac
    29641 ccaaacacta gcacaggccc tctgtagcta tagggtgtta aggcaggtcc acgacgatga
    29701 aacgggtaag ccgattgttg gtccgtttga ccgtgcgact ttcggcaatc cacaaatcct
    29761 gctgacggcc ttttgccagc agtacgcgac gcataaacat gatgttgagc aagagggtga
    29821 acttgccctg ctaactcaag aaattaggcg cttagccagc gaactcagca cgcacaccac
    29881 cgcggcgtgg gttaactata ttcgcggatt aaaagctcag tgggaagtgg atcagagact
    29941 gttcagtaac ttggcgcatc aggaaggaca acgaaaagta caacagcact acattgatct
    30001 cgtggaaaag ttcaccagca gtagccgggg gctgccgaaa accattgaag aatttgagac
    30061 ggtgagagac ctgcatgaga aactctgcca gcaacgcgga gcacttaagc tggatgtgcc
    30121 agaagaggtc aatcacttcc tcaaagccgt agccgggcaa ggtgccacgc tcgatatgtt
    30181 gacacctaat gtactcagtt ggctgactga agaagacgac ccgacgcgct atcgtataag
    30241 acgcctgtaa ggagccgtag tttcaatgga acagattatt cgcgcagtac ttgatgggct
    30301 ggagtcgcag gaggcggtac tgctcacttg gggtaacaca caaggttttt tcactgagac
    30361 cgagctccgt aatactattt ctcaggtcct acagaaccac ccgctcgacg ccaacggcct
    30421 aactagtttt ggcatagtga aagttatgat cgatcgcgga ttgctgctgc gccaccagca
    30481 aaatggtgtt tcggtgtttc gtacccgcat ggcggagtcc gtgcggcttt acagccagtt
    30541 gcgccagatg tttcctaagc acggctttaa tgataactgg cgtactgcac cgacgttggt
    30601 gtccgactat cgcttcttac gccgccagcg caaatacccg aagcgtgacc ggcaggctgc
    30661 tgaagttttg cagcaacttg atgaaatagc aggttttacc tctacgcacc gccaactact
    30721 atcagccctg ctcagccact cgagcggaat gtacaacctc gcctactttc aacaagaagc
    30781 ctgcaaaaac gtactgcgcg gattgcagca gagccagcac cgctcgactg ctaccattgt
    30841 ctgcgccgga actgggactg gtaaaacttt ggcgttctat ctgcccgctc tgacctttat
    30901 cggcagtgaa ttggtcgcca aaagaaaaga cgcggtgaag gctctggcta tctacccgcg
    30961 caacgaactg ctgaaagatc agtttattga aacgttgcgg cagacgcgca aattgaacac
    31021 tgaactggca aagaaaggtg tgcgcaaagt gcgtatcgcc gcattgtttg gtatggtgcc
    31081 aactaataaa gaggcgatcc atcgcgacta catgaaagat gcttggtgca ggcatcgcaa
    31141 tggcatggct tgtctgtaca ttccctgccc gacagagagt tgtcgcggta aaatggtctg
    31201 gcacaccgcc gattatgata aaaaccttga acgcctgcat tgcgacagtt gtagccagac
    31261 catcgagcct gacgaagtga tcctgacgcg taagcgtatg aaaatagaac tgcctgacat
    31321 tctattcact agcaccgaaa tgctgaataa acagatgggc aacccgtttc tggcatcgct
    31381 gtttggcatc ggcaaaaacg tggtcccacc gtcgatgatg ctgcttgatg aggtgcatac
    31441 ctattccggc gtcagcggtg cacagaacgg catgctgatc cgccgctggc gacatctgtc
    31501 cggtgccaca ccgcactttg ttggactgtc cgcaacgttg gagcaggcga cgcgctttat
    31561 ggcttcgtta accggcttgg aagagtacct ggtggcggaa gttagtccga tcgaagacga
    31621 tatggagaca gagggcgcgg agtatatgct ggctctacgt ggcgacccgg cgtcccgctc
    31681 tagccttttg tccaccacca ttcaggcctc catgctaacc aggcgtatgc tcgacaacgc
    31741 cgcagtgccg gtcagcaacg gtatgtacgg cacgcgcagt tttgtattta cagatgatat
    31801 tgacgtcatt aaccgcctct atttccagct actggatgcc gagggtcgtt actcgaatgg
    31861 caatatcaat gccaaaaaag aaccgctggc gatgctgcgc ggcgatgcgc caaatgaaga
    31921 gaaatttacc tttggtcagc aatggccgtt ggctaaaatg attggccata cactcgattc
    31981 tgctgaccgc agcaacgtta agcgcacctc ttcgcaggat gctggcgtcg accatgctgc
    32041 ggatctgatt gtcgctaccg cgtcacttga ggtcggattt aacgacccga acgtcggtgc
    32101 cgttatccag cataaagccc cgcgcgataa tgcccaattc ctgcagcgta aagggcgtgc
    32161 tggacgtcaa cgaaccatgc gcccgtggac cgtggttgtg ctgtccgact atggccgtga
    32221 tcgcatggca tttcagtgct acgaaaacct gtttgagccg gtactaaagg cgcgccaact
    32281 gccggtcggc aacagttacg tactgcgtat gcaggcggcc tttgccacta tggactggct
    32341 ctccagccgt aacgagtatc ttaatcagtg gaatcgtggt atctgggatg acctatccgt
    32401 cccgcaggac aaaggtaagc catctgaagc tcagtcaaag ctggccgatc tgattgaaga
    32461 ccttctgaac agccagaaaa tccagcagga gtttaactca tggttggctg aagcactggg
    32521 gattaaggac gaaaaactgc tgcagtcatt gctgtggcaa cccccacgcg cgattatgac
    32581 cgcgtttctt cctacggtac ttagaagact gcgttccaac tggtcgcgcc ttggcattga
    32641 gcaggccgat aactgccgca aaagcacgcc gatgcctgat tttataccgt ccgcgttgtt
    32701 taacgacctc tgcctgccgg agttacagat taacctgcta ggtgaagtcg gtcaggaacc
    32761 cgatgcgcac agcatgccga ttttgcaggg gatgaaagat tttgccccgg ggcgtatcag
    32821 caagcgcttt gccatcaaaa acattcgcga atgccactgg ctagtgccga aaaaactcga
    32881 gctgaaagat ggttcgcatc cattcccgat tgatgattac tgcccgccag acaaacggga
    32941 gtccatgccc gattgtcaca ttaccactcg cactggcatg caggttatcc cttgcttccg
    33001 cgcctgggaa gtgactgcca gcacgccacc agatgacctt aaactgagtg aaacctcaaa
    33061 cgcgtttctt aactggcaca gcgagatccg tccacctcag aacggcatcc ctgcggaagt
    33121 gccatccaac aatgcctggc aggatatttt ccagcaggtg gagttttaca gccaccagca
    33181 gcactgtccg atcgaagccg tgcgctttgc caccggatca cgtgccaata ttaaattcag
    33241 cgaccagcgt gaagacctgc agattgactt caaatttgaa caccaaggcg aacccgctgc
    33301 gtttggcttc tcgctgtggg tagatgcgat aaaattccag tgtcggttac ctaattttga
    33361 tctcgctagc atcagcaaca accgtgaact ggtggccgga ttgcgcacag cgcgcttcct
    33421 gtatgaagtc agccacgacg aggcaatcag tgttaatact ttcctcggcg gttggctggc
    33481 agaaactgtc ctggcaggta ttggcagcga agcagtattg aaaaactgca ccccggagca
    33541 ggcgttccgc gcaatggaac acaacagcgc aaggatcgcc ttgattgacg ttcctgctcg
    33601 aatcttccag acgttgtatg atgcgcagac cggtgaagag atagagcagg atctacagca
    33661 aagcttgcgt gaactgttgt cccaggctcc ggtgatggca agaattgccg actgggtgga
    33721 actgctgtgg caaccgctgg ataacacctg gttggagtgg ttgaccctca acttcaccaa
    33781 cacactgggt gcggcgctgc tgcaaaccgc tatgcagttg tgcccagatg ccgacgacaa
    33841 cgacctaatc cttgacctca acggtgggcc ggtgcgcact tcgctgctcg cgcctgacca
    33901 gcgcgaaatc tggctgtcgg aaaccaccgt gggcggtggc ggtatcattg agaagatcca
    33961 acagatttac cgtgaagatc cgcgcagttt cttcgagttg ctagacttta acctcagtcc
    34021 aggcgactac gaaacaatgg acaataatgt ctggcacctg ctgcaaacca tggtaaaccc
    34081 gacatcatcc ctgcctgcgt gtatgaacga aatgcgcctt gccaacgatc atgccagcca
    34141 ggttcaggcg cagcgcaact tgctgggaga attgcagcgc agcggcttta tgaccagcca
    34201 cagcttcctg tcggcgatca acacccgcct attacggccg gggaccgatg ccagcagcga
    34261 tgtcttttta ctgcaactgc aacaggattg gcgacagcag gagcagcgac tgggtattga
    34321 actaccacag cgtgtctatg cctttacctg tagtcagtcc agcgcgcttg accagcaact
    34381 acaacaagct agccaagggc agcacaactt ggagagttgg cgtttaaata cctgtaatgg
    34441 cctactgtgg ccacgtggcc ataccattcg tgatagctgg ctaacttggt acaaccctta
    34501 cagctttgcc gggccaaccg agcgcctcct actggcccat gtggtgcagc acagttgtgc
    34561 tcaggtggcg ctcagccaac cagactggat gcagcagtgt caccagcatc tggaaaataa
    34621 tggtaaatgc gaattactag ccgaaccggg cgatgcaata aatgaggcca ttgctactct
    34681 tttagtcacc ccagtagaaa tgtatgggtt gttcttctac ccgcgcgtca gtgccctgcg
    34741 ccgccacaac aatgacacct tcgccatgat tgaaattccg gaggcgatcc aatgaatacc
    34801 cgtcgtattt ttaaatccag aacctcacgc cagagtgaag ttcttgatgt actcagctcc
    34861 ttgttggtag cggagatcgc tcagcccagc gcggaattgt ggatagtctc gccgtggatc
    34921 accgacctcg acctgctgga taatcgcacc ggccgtttcg actacctcga accagcctgg
    34981 ggacagcgtc aggtgcagac ctcagaactg ctggcgcgtg cggtagccaa cggcggtacg
    35041 cttaaagtga tgaccaacga gacccagcat aatgcacgct ttatccgcca gttaaccgaa
    35101 cgcgtcgcca actacggcat gctggataac ctgtacattg gccagaaaga ggtactgcat
    35161 accaaagggt tcctcggaga tcatttcttc ctaagcggtt caatgaatct gacgatcggc
    35221 gggatcgtga tcaatgatga acagattaac ttgaccacag acaaaaccgc catcgtgcag
    35281 gcgttgttag catttaacga cttttaccaa ccggcacagg gaggtgtgca atgaacagcg
    35341 ctgatagcga aaagttcctg cgagcctttt tccatagaga aaacaatatt atctcttggg
    35401 aaaaaatcgc gggtggcctg ctagaagcat caacccaaca gcggttgacc ccctggcttg
    35461 acgatgtccg caacggatgt ttcccggtga tcctgccgcg cgtagtaaag ggcagcaatg
    35521 acaaagtcac ctggtatgca atggccgatg aggaacgaga ctatgcggag ttgcgcgaat
    35581 tattagacgc gcacgtcggt acctcgtggt cagactttaa ccgccagcgc acgatgctgg
    35641 atccgagcga cccggtggaa aaagcgttga tagaccacta tagcgataga gtatataaat
    35701 ttagctctct ttctctgtcg aggatcccgg agattttcaa tggcatagag cgcttacgcc
    35761 aacaactcgc aagccgcccg agttaccagc tcacacttct gcgcccgcgt ggccgtattc
    35821 tgcgtgatat tcgtgccgcc ttgcgccgtc gtgatgtgcc gcagactcgc ctctatctgc
    35881 aggaactacg tgaagctggt ggcataggcc atattaacga actgtttatc gaactgcgct
    35941 gtatggctct ggaaggtgac gccaaaagcc tgctggattc gactcagttg cgacaggtct
    36001 tggatatgcc gcacccgttg gtgttggctg aagaagtaat gacagtcttc tatcagcagt
    36061 ttctgctgaa gtatgagcag ttgagcgacc tcgccgccca acgacaagca tttaataatt
    36121 tgctggtgaa gaacgtaccg cgtctgttcc aggtgacgca gggtataaac acgccatcgg
    36181 caatcaaaaa tattctactg tggttacttt ctgacacaca aagtgatccg gcaagactga
    36241 gggaacaggc acaacaagtg gttgccggta tgaagggggc aacagacgat gaccggcact
    36301 ggatccgccg gttgttggaa gcattcccga ccgttgcagt ggcctcccca ccgacggcga
    36361 gcatcgatga attgcttgcc agagccgatg aatatcctga aagcgttctg aaagagttac
    36421 tattgctacc ggtcagcgct cgcggcgtag ccattgcttt gcaatgtgcc tgggtcgtag
    36481 gcagtgcagg agctgctgtg agtgctgtca gttatctgca acaactggca caacaagagc
    36541 gcgacttgtt actggcgaaa ccggccttcc agcgttatta cactgcctta cagttgatgc
    36601 tgccatccaa acgaacagaa catgatactg caggtatcaa taattggcag gactggttga
    36661 aggaagtgtt aaataaccct caatggtcgg gggcgataga agcggtagaa caggcctgcg
    36721 gccaatggaa cgcagcggct ttccagcccg aggcatttgt ttcgttgctg gataaaacca
    36781 acaactgcgc cagtatggtg ttgcgcgatg ccattccagt actgctcgag tggctggata
    36841 accatcaaac aagtgcttcc tggcagtctg tccggttagc gttgctgcaa caactggcgc
    36901 tggacgacaa tagttctcgc gaagatatct ttactgcctg tgaactagca cgcggttatt
    36961 tagagggcga tagtaatgcg cacgattacc gcgaactaat agaaaacctg cagatgatga
    37021 tcgagagttc aggtaccacc agcgctagcg cctggctgga tttgctggaa ctgttcttgg
    37081 tctacccaac tgctgaccaa cttgccagag aacaattatt caatctggta ctcaacatgt
    37141 tttaccgcaa cccagcccgc tatagtgaat tgcagtggag cctattggct cagtttgctg
    37201 gcgagtgcgg acttgtctgt gaaattcccc cacaaatttt agaggacagt ggggaaaaaa
    37261 atgatgaggt tcgtgtcgga tcaacattga atgacaaggt agtgggcatt tatacattga
    37321 ctgagtcggt agcacagcgt gtgaaaaaac aactactagc cgattatcct caggccgata
    37381 ttcgcctcaa caacgataaa ggcggctcaa ccatgctgcg ttcattggct agagaagcgg
    37441 atgtatttat ttttgcctgg cttagttcta aacattctgc ttattacgcg ataaaggaag
    37501 agcgaggaga taaaccttta ttgcaacctt ccggaaaagg cagtaccagt atgctggact
    37561 gcatacgtag ttatcttaaa ttgtagtaaa ggtaaaaaac ttagctgtaa cctataacgt
    37621 cacagctaag ttcaatctcg ggctattcac cccgataaac acaacccgca ctacaggttt
    37681 ctttcaccat gacagcactc agaattggca actgtgtttt aacctgattc catatccatt
    37741 tggccagtac ttcgctggtt gggttttcca agccagggat gtcgttgaga tagtagtggt
    37801 ctaggcgatc tagcgtcggc ttgaaaatgg ccttgagttc agcgaaatca ataatccaac
    37861 ctgtgtgttt atcaacttca ccagttatct caaggcgcac gacaaaagag tgtccgtgta
    37921 agcggccgca tttatggcct tcaggaacat tgggaagacg gtgtgcggct tcaaatgtga
    37981 aatctttgta taacgtagtt ttcatggtga cctcattttt tgttatacag taacaataag
    38041 aagcaagatt tcctagcatt taagaatgag cattaacacg cttgcgtatt ccgacccaaa
    38101 ccggattgtt ttcgattgtg agcctctttg caggatgagc atgcaactca attggacgtt
    38161 atcgctagcc acagccctac atcgactagt ttcaccgctt cctacgtaag tttaaagatt
    38221 ggcggcaaaa aagagcttca gcattctcat gacgctattt cagttggata actgaaatct
    38281 ccgctacgac aactcaggca ataactaact ggtcttgcat tgtgcgcaga gccagtcggc
    38341 tttactgacc ctgaggatag cattggggat gaagtgctgt agccgtctgg ctaatcagga
    38401 tttacatacg ggcagtgctg tcgcccgttc tccgttagat catcgggccg gtgttcaaac
    38461 cacgggcgtt cggtaatgta ttcaggatgg tattgcagca cgctactgct gtggcaggta
    38521 taacgatcgt caggggtggt cttcgcagta cgggcaccgg cgctcccgtc gaaagcgata
    38581 taggatttgg cgtacatagt attgttcctt accgccagac gacacgcagg ggcagacgtc
    38641 ccggtgctag cagcgtggtt ggatgtgaat ggcaggtcaa tttagtaata tcagaagagg
    38701 gctaagcctg tgctggatgt cattactgtc tgttagtttt cacactatga ggtactgacg
    38761 taaaaagtca gagtgactgg ctgctacggg gtctccctgt taatgggcgt gcagggacct
    38821 cgttgcagcc gtagagcagc aggccatgct tttgctgaat tttggtcata tacgccagta
    38881 cctgtcggat gttggtgata ctgtccggat cgctgtaacg taccggaatg ttgatattgg
    38941 cctcgtaatg ctctttaaac tcacatcggt gataccagcc ttcccggtca gtgatatcgc
    39001 tccagtacaa accggcttgc tgtgcccagg tataggtatt ctgagtttta ctaccatcca
    39061 gccagaacac gacgtgggca tgatagtgat ggtcttctgt ccattcgata acccagaaga
    39121 aaccgaccac cgcttcgagt tgcatcatac ggttcatcag ctttcggata tcccattcga
    39181 gctgctggtg atcctgcagg aaaaacttag cggtatgatc ctggtaaaac agatctgttc
    39241 ggaatgcccg caggcaggaa tagcgctcta ccattttgtc gatatggttg ttcaggagtg
    39301 actgtagaaa ccagtctggc tcccatcgtt gattgttttg cataggatga gtttctttag
    39361 tgctggcaag ggccagccag tggaatgaac caggccttaa ggaaggacca tggcagaggg
    39421 agttgtatgg attagggggg atagaagtaa aaagggagtg cctattaatt atattaatta
    39481 acctaaatta attttcactc cccaataacc ctcaaatccc ttgcaggact atggatgaag
    39541 cctaactcag tggttcagct gtcgtccgcc atgcaaagca gacatactcg tccatcataa
    39601 tggtcagcgc agggttttca gcctgccggt tattgatcca gatatccatc tctttgtctt
    39661 ttttgaacct gaggttgtcg gtccagtcat caaggcaatt ccccagcatc aaatcgtacc
    39721 agcacagcca gaccagcttc agacgcagct cactaccgct gaactggcgg aacaaactga
    39781 tgagcttatt tgccagaggg cgttgtttta aacgcacgca ctcagacagc aaaatcccct
    39841 ccagcatggg atggtgagtc agcagatgct cctgggtaaa taaactgacg ctcaggtctt
    39901 cattaacagt cagtggggct gctgctctac cataaataat ccggtgcagg cactggggga
    39961 caatagcaat aacctcttcc tctcccggaa acttaaggct gccaaccgtc agggtatgat
    40021 ggtggtagat attctggtga cacatccccg atctgaccgt cagcttcgta aaatcagtca
    40081 accagacctg ataatatcta taaagttctt cgtaacgagg cttgtaatct ttcatagttc
    40141 atattcctta aggcttaaat gacagacagc agaaaacctc cggagtaacc ggatagttat
    40201 tattctgctg agggtttagt ctggtgggag gttatttacg agactggtcg attctttcct
    40261 gtagccagtt ttcgacttcg ctttttaacc atcgggagct tcggcccagc ttgatgggtt
    40321 tgggaaagac tccatcctta atgagcttat aaatccactt atctgtcatt tgcagcagac
    40381 tggtgataaa tgccatgtca acgaactggt cttcggtcag tgaggggcgg gtagtcatga
    40441 tttgtatctc cggttaagaa aggtaagccg gattagtgaa gtttgataac gacgactgct
    40501 ccgggacata tccaggtgga gatacgtaaa aaaagagagt tatgattacc tctgttccta
    40561 ctttgatatc cgtgcaggtc tttttcaaat aaagaagata atgaagtgtg atttctggta
    40621 gggccatttt ttagatggga aattgtatgg caactagttg ctgtctatat tggaagcggt
    40681 tgccactatt aatcaaaaaa tgtaagaaga gtgtgtatta actagaatgg taataatttc
    40741 t
//
LOCUS       DAZFWP020000021        40657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFWP020000021 DAZFWP020000000
VERSION     DAZFWP020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.021"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            385..1749
                     /gene="yajR"
                     /locus_tag="ACHK0Z_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             385..1749
                     /gene="yajR"
                     /locus_tag="ACHK0Z_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4501096.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1877..2368)
                     /gene="yajQ"
                     /locus_tag="ACHK0Z_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             complement(1877..2368)
                     /gene="yajQ"
                     /locus_tag="ACHK0Z_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU4501097.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2536..3447
                     /gene="panE"
                     /locus_tag="ACHK0Z_RS05175"
                     /old_locus_tag="pgaptmp_001035"
     CDS             2536..3447
                     /gene="panE"
                     /locus_tag="ACHK0Z_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU4501098.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3410..4000
                     /gene="yajL"
                     /locus_tag="ACHK0Z_RS05180"
                     /old_locus_tag="pgaptmp_001036"
     CDS             3410..4000
                     /gene="yajL"
                     /locus_tag="ACHK0Z_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU4501099.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVANGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4054..5502)
                     /gene="thiI"
                     /locus_tag="ACHK0Z_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     CDS             complement(4054..5502)
                     /gene="thiI"
                     /locus_tag="ACHK0Z_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU4501100.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5708..5950
                     /gene="xseB"
                     /locus_tag="ACHK0Z_RS05190"
                     /old_locus_tag="pgaptmp_001038"
     CDS             5708..5950
                     /gene="xseB"
                     /locus_tag="ACHK0Z_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU4501101.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5950..6849
                     /gene="ispA"
                     /locus_tag="ACHK0Z_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             5950..6849
                     /gene="ispA"
                     /locus_tag="ACHK0Z_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU4501102.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6874..8736
                     /gene="dxs"
                     /locus_tag="ACHK0Z_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             6874..8736
                     /gene="dxs"
                     /locus_tag="ACHK0Z_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU4501103.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8916..9890
                     /gene="yajO"
                     /locus_tag="ACHK0Z_RS05205"
                     /old_locus_tag="pgaptmp_001041"
     CDS             8916..9890
                     /gene="yajO"
                     /locus_tag="ACHK0Z_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU4501104.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9944..10462)
                     /gene="pgpA"
                     /locus_tag="ACHK0Z_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             complement(9944..10462)
                     /gene="pgpA"
                     /locus_tag="ACHK0Z_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU4501105.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10440..11417)
                     /gene="thiL"
                     /locus_tag="ACHK0Z_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             complement(10440..11417)
                     /gene="thiL"
                     /locus_tag="ACHK0Z_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU4501106.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11495..11914)
                     /gene="nusB"
                     /locus_tag="ACHK0Z_RS05220"
                     /old_locus_tag="pgaptmp_001044"
     CDS             complement(11495..11914)
                     /gene="nusB"
                     /locus_tag="ACHK0Z_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU4501107.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11934..12404)
                     /gene="ribE"
                     /locus_tag="ACHK0Z_RS05225"
                     /old_locus_tag="pgaptmp_001045"
     CDS             complement(11934..12404)
                     /gene="ribE"
                     /locus_tag="ACHK0Z_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU4501108.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12493..13596)
                     /gene="ribD"
                     /locus_tag="ACHK0Z_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(12493..13596)
                     /gene="ribD"
                     /locus_tag="ACHK0Z_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU4501109.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSTQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQVL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13600..14049)
                     /gene="nrdR"
                     /locus_tag="ACHK0Z_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(13600..14049)
                     /gene="nrdR"
                     /locus_tag="ACHK0Z_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU4501110.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14200..14739
                     /gene="yajI"
                     /locus_tag="ACHK0Z_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             14200..14739
                     /gene="yajI"
                     /locus_tag="ACHK0Z_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4501111.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSALNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLKSQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15038..15922
                     /gene="tsx"
                     /locus_tag="ACHK0Z_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             15038..15922
                     /gene="tsx"
                     /locus_tag="ACHK0Z_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU4501112.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16099..16446)
                     /gene="yajD"
                     /locus_tag="ACHK0Z_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             complement(16099..16446)
                     /gene="yajD"
                     /locus_tag="ACHK0Z_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU4501113.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16575..17546)
                     /gene="secF"
                     /locus_tag="ACHK0Z_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             complement(16575..17546)
                     /gene="secF"
                     /locus_tag="ACHK0Z_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU4501114.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17557..19404)
                     /gene="secD"
                     /locus_tag="ACHK0Z_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             complement(17557..19404)
                     /gene="secD"
                     /locus_tag="ACHK0Z_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU4501115.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19432..19764)
                     /gene="yajC"
                     /locus_tag="ACHK0Z_RS05265"
                     /old_locus_tag="pgaptmp_001053"
     CDS             complement(19432..19764)
                     /gene="yajC"
                     /locus_tag="ACHK0Z_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU4501116.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19787..20914)
                     /gene="tgt"
                     /locus_tag="ACHK0Z_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             complement(19787..20914)
                     /gene="tgt"
                     /locus_tag="ACHK0Z_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU4501117.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20970..22040)
                     /gene="queA"
                     /locus_tag="ACHK0Z_RS05275"
                     /old_locus_tag="pgaptmp_001055"
     CDS             complement(20970..22040)
                     /gene="queA"
                     /locus_tag="ACHK0Z_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU4501118.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22133..22714
                     /gene="acpH"
                     /locus_tag="ACHK0Z_RS05280"
                     /old_locus_tag="pgaptmp_001056"
     CDS             22133..22714
                     /gene="acpH"
                     /locus_tag="ACHK0Z_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU4501119.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22719..24536)
                     /gene="malZ"
                     /locus_tag="ACHK0Z_RS05285"
                     /old_locus_tag="pgaptmp_001057"
     CDS             complement(22719..24536)
                     /gene="malZ"
                     /locus_tag="ACHK0Z_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU4501120.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(24692..26065)
                     /gene="proY"
                     /locus_tag="ACHK0Z_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             complement(24692..26065)
                     /gene="proY"
                     /locus_tag="ACHK0Z_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU4501121.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(26141..27460)
                     /gene="brnQ"
                     /locus_tag="ACHK0Z_RS05295"
                     /old_locus_tag="pgaptmp_001059"
     CDS             complement(26141..27460)
                     /gene="brnQ"
                     /locus_tag="ACHK0Z_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU4501122.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27867..29162)
                     /gene="phoR"
                     /locus_tag="ACHK0Z_RS05300"
                     /old_locus_tag="pgaptmp_001060"
     CDS             complement(27867..29162)
                     /gene="phoR"
                     /locus_tag="ACHK0Z_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU4501123.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29220..29909)
                     /gene="phoB"
                     /locus_tag="ACHK0Z_RS05305"
                     /old_locus_tag="pgaptmp_001061"
     CDS             complement(29220..29909)
                     /gene="phoB"
                     /locus_tag="ACHK0Z_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU4501124.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            30099..31301
                     /gene="sbcD"
                     /locus_tag="ACHK0Z_RS05310"
                     /old_locus_tag="pgaptmp_001062"
     CDS             30099..31301
                     /gene="sbcD"
                     /locus_tag="ACHK0Z_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU4501125.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITADEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31298..34441
                     /gene="sbcC"
                     /locus_tag="ACHK0Z_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             31298..34441
                     /gene="sbcC"
                     /locus_tag="ACHK0Z_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU4501126.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34567..35751
                     /gene="araJ"
                     /locus_tag="ACHK0Z_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             34567..35751
                     /gene="araJ"
                     /locus_tag="ACHK0Z_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU4501127.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35895..36803)
                     /gene="mak"
                     /locus_tag="ACHK0Z_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             complement(35895..36803)
                     /gene="mak"
                     /locus_tag="ACHK0Z_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU4501128.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36928..37839
                     /gene="rdgC"
                     /locus_tag="ACHK0Z_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             36928..37839
                     /gene="rdgC"
                     /locus_tag="ACHK0Z_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4501129.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37917..38000)
                     /locus_tag="ACHK0Z_RS05335"
                     /old_locus_tag="pgaptmp_001067"
     CDS             complement(37917..38000)
                     /locus_tag="ACHK0Z_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501130.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37997..38338)
                     /locus_tag="ACHK0Z_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /pseudo
     CDS             complement(37997..38338)
                     /locus_tag="ACHK0Z_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38486..38770)
                     /gene="ppnP"
                     /locus_tag="ACHK0Z_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             complement(38486..38770)
                     /gene="ppnP"
                     /locus_tag="ACHK0Z_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU4501131.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38842..39519)
                     /gene="aroM"
                     /locus_tag="ACHK0Z_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             complement(38842..39519)
                     /gene="aroM"
                     /locus_tag="ACHK0Z_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU4501132.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDIQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39777..39968)
                     /gene="yaiA"
                     /locus_tag="ACHK0Z_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             complement(39777..39968)
                     /gene="yaiA"
                     /locus_tag="ACHK0Z_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU4501133.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(40018..40542)
                     /gene="aroL"
                     /locus_tag="ACHK0Z_RS05360"
                     /old_locus_tag="pgaptmp_001072"
     CDS             complement(40018..40542)
                     /gene="aroL"
                     /locus_tag="ACHK0Z_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU4501134.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT        10123 a        10858 c        10246 g         9430 t
ORIGIN      
        1 catatatttc cataataatt aaccaccaaa tatccattta atgaataaat acagtgccat
       61 aaaggtccaa gtaataagca cacatttagc ctgaaacatt atcatccatg tcaccaaatc
      121 gtttatttag tgagataaaa ctagcatatt cgtaagataa aaaatgcaca tggcaatcaa
      181 gaaatgatat tattactttg ttgatgtaaa actatttagt tttaaaattc attttttcaa
      241 catatagagc attcatttta tgaatgcaaa aaaacatacc tgacacacaa attactctcc
      301 tctgtaatat tttctcatta acactctgtt tacccaacat gcgctccgca ctacactatg
      361 tgggcatttt tttaaggggt ggtaatgaac gattataaaa tgacgccagg tgagaggcgc
      421 gcgacctggg gtttagggac cgtattctcg ttgcgcatgc tgggcatgtt tatggttctg
      481 ccggttctga ccacgtacgg catggctctg caaggcgcca gcgaagcatt aatcggtatt
      541 gccattggta tttatggtct gactcaggcc gtttttcaga ttccgtttgg cctgctttcc
      601 gaccgcattg gtcgcaaacc attaattgtc ggtgggctgg cggtgtttgc cgccggtagc
      661 gttatcgctg cgctttccga ctccatctgg ggaattattc tgggccgggc gctacaaggc
      721 tctggagcaa ttgccgccgc cgttatggcg ctgctttccg atctcacgcg cgaacaaaac
      781 cgcaccaaag cgatggcgtt tatcggcgtg agctttggca ttacctttgc cattgcgatg
      841 gtgcttggcc cgatcatcac tcacaaactt gggctgcacg cgctgttctg gatgatcgct
      901 attctggcaa caaccggcat tgcgttgacc atttgggttg tgcccaacag tagcactcac
      961 gtacttaatc gtgagtccgg aatggtgaaa ggcagtttca gtaaagtgct ggcggaaccg
     1021 cggctgctaa aactcaactt tggcattatg tgtctgcaca tgcttctgat gtctacgttt
     1081 gttgccctgc ccggacagct agctgatgcg gggttcccgg cggctgaaca ctggaaggtc
     1141 tatctggcga cgatgttaat cgcctttggc tcggtcgtgc ctttcattat ctacgctgaa
     1201 gttaagcgca aaatgaagca agtctttgtc ttctgcgtcg ggttgatcgt ggttgcggaa
     1261 attgtgttgt ggaacgcaca aacgcagttc tggcaactgg tggtcggcgt gcagcttttc
     1321 tttgtggcgt ttaatttgat ggaagccctt ctgccctcac ttatcagtaa agagtcgcca
     1381 gcaggttaca aaggtacggc gatgggtgtc tactccacca gccagtttct tggcgtggcg
     1441 attggcggtt cgctgggcgg ctggattgac ggcatgtttg acggtcaggg ggtatttctc
     1501 gctggcgcaa tgctggccgc agtgtggctg gcagtcgcca gtaccatgaa agaaccgccg
     1561 tatgtcagca gtttgcgcat tgaaatcccg gcggacattg ccgcaaacga agcgttaaaa
     1621 gtgcgtttgc tggaaactga aggcgtcaaa gaagtgttga ttgcagaaga agagcattca
     1681 gcttatgtga aaatcgacag caaagtgaca aatcgctttg aggttgaaca ggcaattcgt
     1741 caggcataaa aaacgcggcg aattgcctgc tgcgctacgc ttaccaggcc tacaaagttc
     1801 ttgagttaca aaccgttgta ggccgaatca ggcgttcatg ccgcatctga catgaacaaa
     1861 acgcacattg tcgcgattaa tcgcggaagt ttttgaactg gaacggctgg ccgagatcgc
     1921 caccacgtac catcgccatg acagcctgca aatcatcacg agatttgcca gtcacacgga
     1981 tctcatcgcc ctgaatttgc gcctgcactt tcagtttgct gtctttaatc atcttgacga
     2041 ttttcttctg ggtcgcgctc tcaatgccct gtttcagttt cgcttccaca aaccaggttt
     2101 taccgctatg aacgatattt tccggtacat ccagtgaact gccttcaatg ccgcgcttca
     2161 gcagcttggc acgcagaata tccagcaact gattgacctg gaagtcggac tcgctcaaca
     2221 ctttgatggt tttgctggcg tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa
     2281 aacgggactc cacttcgcga ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa
     2341 cttcagagac aatatcgaaa gatggcatct tttcctctcc cttcattttt gatgcgaagc
     2401 ataatacctg caaagttaaa taaccaacag tcctgcacca ggacgtatcc cttaattgtg
     2461 catagtcgca acagcatgca caattaaggg atagccttat tgaacacccg gagtggttgc
     2521 gggtgaggag gaacaatgaa aattaccgta ttgggatgcg gtgccttagg gcaattatgg
     2581 cttacagcac tttgtaaaca gggtcacgaa gttcagggct ggctgcgcgt accgcaacct
     2641 tattgtagtg tgaatctggt tgagactgat ggttcgatat ttaacgaatc gctgaccgcc
     2701 aacgatcccg attttcttgc caccagcgat ctgctcctgg tgacgctgaa agcatggcag
     2761 gtttacgatg ccgtcaaaag cctcgcgtcc acactgcctg taaccacgcc aatactgtta
     2821 attcacaacg gcatgggcac catcgaagag ttgcaaaaca ttcagcagcc attactgatg
     2881 ggcaccacca cccatgccgc ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga
     2941 atcacgcata ttggtccggc acggcaacag gatggcgatt acagttatct ggcggatatt
     3001 ttgcaaaccg tgttgcctga cgttgcctgg cataacaata ttcgcgccga gctgtggcgc
     3061 aagctggccg tcaactgtgt gattaatcca ctgaccgcca tctggaattg cccgaatggt
     3121 gaattacgtc atcatccgca agaaattatg cagatatgcg aagaagtcgc ggcagtgatc
     3181 gagcgcgaag ggcatcatac ttcagcagaa gatttgcgtg attacgtgat gcaggtgatt
     3241 gatgccacag cggaaaatat ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc
     3301 gaaatcgact atatcaatgg ttttctctta cgccgcgccc gcgcgcatgg gattgccgta
     3361 ccggaaaaca cccgcctgtt tgaaatggta aaaagaaagg agagtgaata tgagcgcatc
     3421 ggcactggtt tgcctcgccc ctggtagtga agagactgaa gccgtcacca ctatcgatct
     3481 gctggttcgc ggcggtatca aagtcaccac tgccagcgtc gccagcgatg gcaacctggc
     3541 cattacctgc tcgcgcggcg tgaagctgct ggcggatgcg ccgctggtcg aggtggctaa
     3601 tggcgaatat gacgtgatcg tactgcctgg tggcattaaa ggcgcagagt gttttcgcga
     3661 tagtaccctg ctggttgaaa ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc
     3721 tatttgcgcc gcgccagcca ccgtgctggt accgcacgat atcttcccga ttggcaatat
     3781 gaccggcttc ccgacgctga aagacaaaat tcccgccgaa caatggcagg acaagcgcgt
     3841 cgtctgggat gcacgggtaa aattgctgac cagccagggg ccgggtacgg ctattgattt
     3901 tggtctgaaa attatcgacc tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca
     3961 actggtgatg gcggcaggga tttataatta ttacgagtag tgtcggatgc ggcaaacgtc
     4021 gcatctgacc agatgcgacg taaaaaccac aaattatggg cgatacacct tcacattgtt
     4081 aaagccctgc tcgcgcagat agagcgcctg cagacggctc atcaccccgc gctcacacca
     4141 cagtagccag gttctgttct ggtcgagatc gccaaacttg gtgctcagtt tatagaacgg
     4201 cagagaaact acatcaatcc cttcgacttt cagtggctta tcttcctgtt catcgataga
     4261 acggatatcg aggatcacgt cgttcgggcc gaagccattg acggtttcca cttccaccac
     4321 ttcctgctcg gtctgctggg cgatttcgcg gatatcaacg ttattcgctt cttcaaccac
     4381 tttatcgaga atgctgaagt cgaatttctc ttcttccgct tcaatcttcg acttaaccgc
     4441 tttcaccgtc gggcttttgg agataacacc gcaatattcc ggcatcgtgc gagcaaagtc
     4501 ttcggtgcca atctggcgag ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg
     4561 acgcaggatc agcgtatcgg agacgttatc aatcaggcgc aggttggtca gcgtctgact
     4621 ggacacctgg ccgagcgctt cgccggtgac cagcgcctgt acgccgtaac gttcagccac
     4681 tttagacgcg gcacgcacca tcatacgttt gaggataacg cccatctgac cgtcgtcgat
     4741 tttctcgaga atttccccga cgaccggttc gaaattaata gcgacaaaac gcacgcggtg
     4801 ggagctgcca aaacggttcc acagataatg cgccacctga cgcacgccaa tttcatgcgc
     4861 cgcgccgcca aggttaaaga aacagtaatg cacgcggcag ccgcgacgca tcaacatata
     4921 actggaaaca ccggagtcga aaccaccgga aatgagcgac agcacatctt cctgggtgcc
     4981 gatcgggaaa ccgccaatac cttcgtagcg gcctttaatc agcaggagac gatcgtcttc
     5041 cacttccaga tggacagtca cttccggatt ggtcagcttc acgcgcgcgg actcaatatg
     5101 ctgatttaaa ccgccgccga cgtaacgttc cacatcaatc gagctaaaat catgtttgcc
     5161 acgacgcttc acgcgtacgc agaaggtttt gccttccagc tgatcgcgat actgaaccaa
     5221 cgctttctca aaaatatcgt gcatgtcggt aaacggcacg tcttcgactt cgagaatatg
     5281 gtggataccc ggaatacggg tcagggcgtc gcgaatagcc agacgctggt tttcatcttt
     5341 tgcgcgaact tcgatgttat cccagtggcg gacgacagcg agcgtctcat catagtgctt
     5401 taaaacgtta cgaatgttcc cggtaaggat ttttataaag cgcaagcgca cagattggct
     5461 tttgatggtg atttccggga acaatttaat gataaacttc atggcggcaa tggttcgttg
     5521 gcaagcctta agcgacttgt atagggaaaa atacagcagc ccacacctgc ggctgcatcc
     5581 aggcgcggaa gtataccact aacatcgctt tgctgcgcac atcaccttac cattgcgcgt
     5641 tatttgctat ttgccctgag tccgttacca tgacggggcg aaaaatattg agagtcagac
     5701 attcattatg ccgaagaaaa atgaggctcc cgccagcttt gaaaaggcgc tgagcgagct
     5761 ggaacagatt gtaacccgtc tggaaagtgg cgacctgccg ctggaagagg cgctgaacga
     5821 gttcgaacgc ggcgtgcagc tggcacgtca ggggcaggcc aaattacaac aagccgaaca
     5881 gcgcgtacaa attctgctgt ctgacaatga agacgcctct ctaacccctt ttacaccgga
     5941 caatgagtaa tggactttcc gcagcaactc gaagcctgcg ttaagcaggc caaccaagcg
     6001 ctgagccgtt ttatcgcccc actgcccttt cagaacactc ccgtggtcga aaccatgcag
     6061 tatggcgcat tattaggtgg taagcgcctg cgaccttttc tggtttatgc caccggtcat
     6121 atgtttggcg ttagcacaaa cacgctggac gcacccgctg ccgccgtaga gtgtatccac
     6181 gcttactcat taattcatga tgatttaccg gcgatggatg atgacgatct gcgtcgcggt
     6241 ttgccgacct gccatgtgaa gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta
     6301 caaacgctgg cgttctcgat tctaagcgat gccaatatgc cggaagtgtc ggatcgcgac
     6361 agaatttcga tgatttctga actggcgagc gccagcggta ttgccggaat gtgcggtggt
     6421 caggcattag atttagacgc ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt
     6481 catcgtcata aaaccggcgc attgattcgc gccgccgttc gccttggtgc attaagcgcc
     6541 ggagataaag ggcgtcgtgc tctgccagta ctcgacaagt acgcagagag catcggcctt
     6601 gccttccagg ttcaggatga catcctggat gtggtaggag atactgcaac gttgggaaaa
     6661 cgccagggtg ccgaccagca acttggtaaa agtacctacc ctgcacttct gggtcttgag
     6721 caagcccgga agaaagcccg ggatctgatc gacgatgccc gccagtcgct gaaacaactg
     6781 gctgaacaat cactcgatac ctcggcactg gaagcgctag cggactacat catccagcgt
     6841 aataaataaa caataagtat taataggccc ctgatgagtt ttgatattgc caaatacccg
     6901 accctggcac tggtcgactc cacccaggag ttacgactgt tgccgaaaga gagtttaccg
     6961 aaactctgcg acgaactgcg ccgctattta ctcgacagcg tgagccgttc cagcgggcac
     7021 ttcgcctccg ggctgggcac ggtcgaactg accgtggcgc tgcactatgt ctacaacacc
     7081 ccgtttgacc aattgatttg ggatgtgggg catcaggctt atccgcataa aattttgacc
     7141 ggacgccgcg acaaaatcgg caccatccgt cagaaaggcg gcctgcaccc gttcccgtgg
     7201 cgcggcgaaa gcgaatatga cgtattaagc gtcggtcatt catcaacctc catcagtgcc
     7261 ggaattggta ttgcggttgc tgccgagaaa gaaggcaaaa atcgccgcac cgtctgtgtc
     7321 attggcgatg gcgcgattac cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat
     7381 atccgtcctg atatgctggt ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtc
     7441 ggcgcgctca ataaccatct ggcacagctg ctttccggta agctttactc ttcgctgcgc
     7501 gaaggcggga aaaaagtttt ctctggcgtt ccgccaatta aagagctgct caaacgtacc
     7561 gaagaacata ttaaaggcat ggtagtgcct ggcacgttgt ttgaagagct gggctttaac
     7621 tacatcggcc cggttgacgg tcacgatgtg ctggggctta tcaccacgct gaagaacatg
     7681 cgcgacctga aaggcccgca gttcctgcat atcatgacca aaaaaggtcg tggttatgaa
     7741 ccggcagaaa aagaccccat cactttccac gccgtgccta aatttgatcc ctccagcggt
     7801 tgtttgccga aaagtagcgg cggtttgccg agctattcaa aaatctttgg cgactggttg
     7861 tgcgaaacgg cagcgaaaga caacaagctg atggcgatta ctccggcgat gcgtgaaggt
     7921 tccggcatgg tcgagttttc acgtaaattc ccggatcgtt acttcgacgt ggcaatcgcc
     7981 gagcaacacg cggtgacctt tgccgccggt ctggcgattg gtgggtacaa acccattgtc
     8041 gcgatttact ccactttcct gcaacgcgcc tatgatcagg tgctgcatga cgtggcgatt
     8101 caaaagctcc cggtcctgtt cgccatcgac cgcgcgggca ttgttggtgc tgacggtcaa
     8161 acccatcagg gcgcttttga cctctcttac ctgcgctgca taccggaaat ggtcattatg
     8221 accccgagcg atgaaaacga atgtcgccag atgctctata ccggctatca ctataacgat
     8281 ggcccgtcag cggtgcgcta cccgcgtggc aacgcggtcg gcgtggaact gacgccgctg
     8341 gaaaaactac caattggcaa aggcattgtg aagcgtcgtg gcgagaaact ggcgatcctt
     8401 aactttggta cgctgatgcc agaagcggcg aaagtcgccg aatcgctgaa cgccacgctg
     8461 gtcgatatgc gttttgtgaa accgcttgat gaagcgttaa ttctggaaat ggccgccagc
     8521 catgaagcgc tggtcaccgt agaagaaaac gccattatgg gcggcgcagg cagcggcgtg
     8581 aacgaagtgc taatggccca tcgtaaacca gtacccgtgc tgaacattgg cctgcctgac
     8641 ttctttattc cgcaaggaac tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc
     8701 ggtatggaag ccaaaatcaa ggcctggctg gcataatccc tactccactc ctgctatgct
     8761 taacaaatta ttcatagact ctaaataatt cgagttgcag gaaggcggca aacgagtgaa
     8821 gccccaggag cttacataag taagtgactg gggtgaacga atgcagccgc agcacatgca
     8881 acttgaagta tgacgagtat ggcaggagtg gcagcatgca atacaacccc ttaggaaaaa
     8941 ccgaccttcg cgtttcccga ctttgcctcg gctgtatgac ctttggcgag ccagatcgtg
     9001 gtaatcacgc atggacactg ccggaagaga gtagccgtcc catcattaaa cgcgcgctgg
     9061 aaggcggcat aaatttcttt gataccgcca atagctattc cgatggcagc agcgaagaga
     9121 tcgttggtcg cgcactgcgg gatttcgccc gtcgtgaaga cgtggtcgtt gcgacgaaag
     9181 tgttccatcg cgttggtgat ttaccggaag gattatcccg tgcacaaatt ttgcgctcta
     9241 tcgacgacag cctgcgccgt ctcggcatgg attatgtcga tatcctgcaa attcatcgct
     9301 gggattacaa cacgccgatc gaagagacgc tggaagccct gaacgacgtg gtaaaagccg
     9361 ggaaagcgcg ttatatcggc gcgtcatcca tgcacgcttc gcagtttgct caggccctga
     9421 agctacaaaa acagcacggc tgggcgcagt ttatcagtat gcaggatcac tacaatctga
     9481 tttatcgcga agaagagcgc gagatgctgc ctctgtgtta tcaggagggc gtggcggtga
     9541 ttccgtggag cccgctggcg cgggggcgac tgacgcgtcc gtggggagaa actaccgcac
     9601 gactggtgtc tgatgaggtg gggagaaatc tctataaaga aagcgatgaa aatgacgcgc
     9661 agatcgcaga gcggttaacg ggcgtcagtg aagaactcgg tgcaacacga gcacaagttg
     9721 cgctggcctg gttgttgagt aaaccgggca ttgccgcacc gattatcggt acatcgcggg
     9781 aagaacagct tgatgagctg ctgaacgcgg tggatatcac tttgaagcca gaacagattg
     9841 ccgaactgga aacgccgtat aaaccgcatg cggtcgtagg atttaaataa gagtccaggc
     9901 ctgatgagac gtgacaagcg tcacatcagg catcggtgca caactacgac agaataccca
     9961 gcggccagtg atgaccgata aagtacagga tgcctgcgga aatcaccccg gcgacaatat
    10021 cgtcgatcat gatccccatg ccgccatgta cattgcgatc aaaccagcgg atcggccacg
    10081 gcttccacat atccagaata cggaaaatca caaacccggc ggcaacccac tgccagtcat
    10141 tggtcggcag cgccatgagc gtgatccaca taccaataaa ttcgtcccag acaatgctgc
    10201 cgtgatcgtg cacgcccata tctttcgccg tttgatgaca aagatagacg ccgatacaga
    10261 tccccaacat caccaccagc gagtagagct gccagggtaa aaaggtcatc agataccaga
    10321 acggaatcgc tgccagcgat cccatcgtgc caggaacgat tgggcttaat ccacttccga
    10381 atccgacagc aagcagatgc cacggattac tcatcttcag gcgacttttc gcgacatctt
    10441 tatggcgtgg caaaatggtc atatcctttc cagtcaaacg tgacaggttc gccgtcacga
    10501 ataaaacaaa gcccttcgat atcggcggtc atttgcccga tacaggtaaa cggtacgccc
    10561 aggtgtccga gagccacatc cagcgcgcca cggttcagtt ccggcacagt gaaacacaac
    10621 tcgtaatctt caccgccaga gagcgcccag cgcagcgcct gttccggttc aacatggcga
    10681 gaaagcgcat cagaaaacgg cagcaatgcc aggtcaatac gtgcgccgca gtcgctggct
    10741 ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga gatcgatagc tgaatttgcc
    10801 agatcgcgca gtgcctgtcc ctgtaaaata cgcggcgatg gacggagatg acgtttgatc
    10861 aagtagtccg catctttagc atcggcaacc tgcaaacggt tttgcaaaat cgccagcccg
    10921 gcggcgctat cgcccggtgt accggtcaca tagatccagt cacccggttt cgctccagag
    10981 cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac ccaacgtcat tgataatggc
    11041 ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat aattgagaag atcaaacaaa
    11101 ctgtcgctga aggactcaag ccacgcttcg tctacgtccg gtaaggttaa tgccagcgtc
    11161 agccaggccg gatcggcccc catcgctgcc agatcgctta ggttcaccgc cagtgcttta
    11221 tacgccagat cagcaggatc gatatcatgg aggaagtggt tgcccgccac cagcgtatca
    11281 gtgctgatcg ccagggtctg tttctcgggg atattgagaa gagcgcaatc gtcgccgatg
    11341 cccagttcga catcaagacg agaacttctt acacggtcaa aataacgggc aatcagggag
    11401 aactcgccac atgccatacg ttatgcctca gcaggtaaaa aagaaaaggc cggcgatcgc
    11461 ggaacggtct tccgtgaatc taccggcctg gatatcactt tttgttaggg cgaatcacag
    11521 gtgctgcttt atcgagtacg ccgttgacga acttatggct gtcttctgcg ccgaacgatt
    11581 tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg cacatcgcta cgtttagaca
    11641 gctcgtacag cgcaatgcgc agtactgctt tttctacctg tcccagttct tccagcaggc
    11701 gggacaggta tggcttcatc agtccgtcga ggtatgcggt gttagtcgcc accccggcca
    11761 gcagctcacg gaagtacagg acgtcaacgt cttttacatc ctgttcagcc aggaactggt
    11821 attcaacatc agcgatgtcg ttctgggaca actgccagga gtagagcgcc tggacggcac
    11881 actcacgagc gcggcgacga gcagcaggtt tcacggattt ccccttacta atttcaggcc
    11941 ttgatggctt tcaatacatt aatcatttca agcgcggtca gtgcagcttc tgcacctttg
    12001 ttgccagctt tggtgccagc acgttcgatc gcttgttcaa tgctttcagt ggtcagaacc
    12061 ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg ccaggccgtt gcttgcacca
    12121 ccagcgacat attcaaagtg ggcagtgcca ccacgaataa ccgtacccag cgcaatcacc
    12181 gcgtcgtatt taccggtttt agccagtgca cccgccgcca gcggcagctc ataggcacca
    12241 ggcacccaaa caacggtaat gttttcatct tttacctgac cgatacgttt cagtgcgtca
    12301 attgcacctt ccagcaggct gtcattgata aagttgttga aacgcgcgat ggtgatggcg
    12361 acgcgagcgt ccggggtagc aacgttagct tcaataatgt tcattttctt ccttcgggtt
    12421 cgagtatggc cccgcagggg ggcggatttt agcataatat ttcgtgcgct gcttcccttt
    12481 cgagccggga gatcatgcac ccactaaatg caggcaaaca tccgggccta catgacgtat
    12541 ctctttgaat ttaaattggg gggcgtcggc taatttctca agccctggca gcgagcataa
    12601 tccacgggcg tcgctgccta atagtttagg tgcgatatag acaatcagct catcgactaa
    12661 acccacctgc agcaatgcgc cagcgagcgt tggccccgct tccacccaga tgctgttaat
    12721 ttgctgttta cccagttgca tcatcagtac aaccagatcc agatgacctt tatgctctgg
    12781 aatcagcaag gtacgcaccg tttccggcca ctcacgagaa tcttcctgcg tacgcgcgaa
    12841 ccaggtttcg ccgggctgct gcacaatgcg atgttccggc gtcacgcgat tttggctatc
    12901 aatcacaata cgtaccggct gacggagatt ttgttgcggg tagagcacct gagtttgttc
    12961 atccagttcg gaccaacgca ccgttaaggc aggatcatcc gccagcaccg tggcgctgct
    13021 ggttaaaatg gcatgacttt gcgcgcgcag tcgttgtacg tcgcgccgcg cctgagttga
    13081 agtgatccac tggctttcgc cgctcgccat cgccgtgcgg ccatcaagcg atgcgccaag
    13141 ttttaactga atataaggaa agccggtgcg catccgcttg agaaagcctt tattcaattg
    13201 ctcggcttca ctcatcatca gcccgtggct gacgtcaatg ccagcctgtt gcagacggta
    13261 aagtccacgc ccagcgacct gcgggttagg atcttgcatc gctgcgacca cgcgcgctac
    13321 gccagcggcg atgagtgcgt cacagcacgg tggcgtacga ccatgatggc tacagggttc
    13381 gagtgtgaca taggcggtcg cacctttggc tttttcaccc gccatacgca acgcgtgtac
    13441 ttcggcatgt ggttcacccg ctcgttggtg gtaaccttca ccgacaattt cgccatcttt
    13501 gacaatgacg cacccgacat tcgggttggg atgcgtggta aaacgtcctc gttgcgccag
    13561 ctttagcgcc cgcgccatgt aatactcgtc ctgcacggct tagtcctcca ggcgcgcgat
    13621 ctcttcgcca aattctttga tatcttcgaa actgcggtag acagaggcaa aacggatata
    13681 ggcgacttta tcgagctttt tcaattgctc catcaccaga ttgccaatca tcttgctcgg
    13741 tacttcgcgc tcaccggtgg cgcgcaactg cgatttaata tgattgattg ccatttcaac
    13801 gtcatcggaa ctcaccggac gtttttccag cgcccgcagc attccgctgc gcaatttctc
    13861 ttcattaaac ggttcacgca cgtcgttgct ttttacaaca cgcggcataa ccagctccgc
    13921 cacttcaaag gtggtgaaac gttcattaca caccagacac tgccgacggc ggcgtacgga
    13981 tgaaccctcg cccacgagac gagagtcaat taccttagtg tccacggcga aacagaatgg
    14041 gcaatgcata cggcgtcctg accaggtggt taacggaaat ctattttacc ctgaactgac
    14101 gatacaacaa aggcgcaacg cttttgagat aaaggatcga tgctttcgct gcttttaccc
    14161 tctaccatta ggctaattac gacgagaaag ggacgaccta tgaatactaa cgtttttcga
    14221 ctgctcctgc tgggaagcct gttcagcctt agtgcctgtg tgcagcaaag cgaagtgcga
    14281 cagatgaaac acagcgtcag cgcgctgaac caggagatga cgcaactcaa tcaagaaaca
    14341 gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca gcagtggggt ttaccttctg
    14401 cctggagcga aaacaccggc aagactgaaa agccagatcg gtactttacg tatgtcgctg
    14461 gtgaatatta cgcctgatac agatggcacc accctgacgc tacgtattca gggtgagtcg
    14521 aatgatccct tgcccgcatt cagcggaact gttgaatatg ggcaaattca gggaacaata
    14581 gacaactttc aggaaatcaa tgtgcaaaat caattgatta acgcccctgc cagcgtcctc
    14641 gcccccagcg atgttgatat tccgttacag ttaaagggga tttctgtgga tcagttaggc
    14701 tttgtacgta tccacgacat tcaacctgtt atgcagtaaa cgtatttcgg ggcgattttg
    14761 tgcgtcccgc aacatctttc cccgtcattt tgttactctg cttacatcac ccagattgat
    14821 agtaaaagtt tgcaacaagg gtgaaagtca gtacaatccc tgcccgaatg tgtgtaaacg
    14881 tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg aaacatattt ttgtgagcaa
    14941 tgatttttat aataggctcc tctgtatacg aaatatttag aaacgcaatt tgcgcctttt
    15001 tcactcccgc aagggatttt caaacagtgg catacatatg aaaaaaacat tactggcagc
    15061 cggtgcggta ctggcgctct cttcgtcttt tactgtcaac gcagctgaaa acgacaaacc
    15121 gcagtatctt tccgactggt ggcaccagag cgttaacgtt gtcggaagct atcacacccg
    15181 tttcggaccg cagatccgca atgataccta ccttgagtac gaagcattcg ctaaaaaaga
    15241 ctggttcgac ttctatggtt atgcggatgc gccggtattc ttcggcggta actccgatgc
    15301 aaaaggtatc tggaaccacg gttctccgct gtttatggaa atcgaaccac gtttctccat
    15361 cgacaagctg accaatactg accttagctt cggtccgttc aaagagtggt acttcgcgaa
    15421 caactacatt tacgacatgg gtcgtaataa agatggtcgc cagagcacct ggtacatggg
    15481 tctgggtacc gacatcgata ctggtctgcc gatgagcctg tccatgaacg tctatgcgaa
    15541 ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag tgggacggtt accgtttcaa
    15601 aattaaatac tttgtgccga ttaccgatct gtggggcggt cagctgagct acatcggctt
    15661 caccaacttc gactggggtt ccgatttggg ggatgacagc ggtaacgcaa tcaacggtat
    15721 taagacccgt actaataact ctatcgcttc cagccatatt ctggctctga actacgatca
    15781 ctggcactac tctgtcgtag ctcgttactg gcacgacggt ggtcagtgga acgacgatgc
    15841 agaactgaac ttcggcaacg gcaacttcaa cgttcgctct accggctggg gtggttacct
    15901 ggttgtaggt tacaacttct gattatgaaa atgccgggat ttattcccgg catttcagat
    15961 tgttaacaac gtgcgctttg ttcatgccgg atgcggcgta aacgctttgt ccgacccaca
    16021 aaagcacgta aattcaatat attgctgaaa taacgtagtc ctgataagcg tagcgcatca
    16081 ggcaatttta cgttttaatc acttcttctt gttcatcatc gctttcaggt cagcgaatgg
    16141 gttgtacttc gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc
    16201 atactgatcc gcttcggtat atttcgaatg ctcatgatcg tggcaataga gacaaagcaa
    16261 ttcccagtta ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt ggtgaacagt
    16321 aagttcacgc aggttggaat agacaaactc gcgggaacag cgaccgcaga cccacggata
    16381 gatttttaat gctttttcgc gatagccgct ttctaaccgc gcatagtttt ttgggatgat
    16441 agccatgaaa agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg
    16501 attttcgcaa tctccataca tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat
    16561 caacgggaac ttgattaagg cagaattgac ggctgatccg ccccttcttt ttccactttc
    16621 tgctgcaaca tgtgttcgcg cttcataccc agtttcagag ccaacgcaga tgccacatag
    16681 atggaagatg cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa gccttccagt
    16741 accggaccac cgaagaggta cagcatcagg ataaccatca aggtagtacc ggatgtgatc
    16801 aaggtacggt gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga
    16861 cggatcttgc ggaagttttc acgaatacgg tccgatacca cgatactgtc gttaagcgag
    16921 taaccgataa ccgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac
    16981 gacaaaatac ccagcgtaat gataacgtcg tgcgccagcg caataaccac ccctgccgcc
    17041 agtcgccact caaagcggaa acctacgtac acgaggatag acagcagcgc tgccatcaac
    17101 gccatcgcac cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc
    17161 ttcactgctg cattctgatt ggtggattcg ttaatcacct tcagaacctg gctgcccagc
    17221 acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta
    17281 ccaaagtttt gcagcattgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg
    17341 tcaatttcag ccggtttttc gagcgtaatt tcgataaccg taccaccggt aaaatccagc
    17401 ccccagttaa agccgcgcac gcccataata acgatagcag cgattaacag cagaccagag
    17461 atgccgaaag cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt
    17521 tgttcaacag tatattcctg tgccacatcg cactcctcag attgacagct tcttgacgcg
    17581 cttgccgcca tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga
    17641 cgtcgccaca ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc
    17701 gtacaggatg atgactttaa tcagcgtggt gatgttcgca tcgaagatag aactgaatgc
    17761 gccacgataa ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt
    17821 aatacgttcg ttgatcagta cgttcgcatc gaccgccacc gcaagggtta agacgatacc
    17881 cgcgatacct ggcatactca gcgttgcgcc tggcaacagc gacataatgc cgacgattaa
    17941 gatcaagttg gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat
    18001 catgaacaga atagaaacca gcagaccggc aaggcaagcc tccagcccct gttcaatgtt
    18061 ctgcataccc agggttgggc caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa
    18121 cgcaccggca cgcagcagca gtgacagctg acgggcttcg ttcgggttgt tgatgccggt
    18181 gatacggaag ctgttaccca gacgagactg gatgttggcg atgttaatca cctcttcctg
    18241 tttcaccaga accgcacgac catttgcatc tttcttaccg ctgtctttgt actccacaaa
    18301 cagggttgcc atcggtttgc cgatgttgtc cttagtgaag ttagacatga tgttaccacc
    18361 agcgctatcg agcgagatgt taacctgcgg ttggttgtat tcgtcctggc tggaagtgga
    18421 gtcggtgata tggtcaccgg tcagaattac gcgtttgtac agcacaactg gctgaccttc
    18481 gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctggtc
    18541 aacgttggtg tttaccagac ggaattccag cgttgccgtc gcacccagaa tctctttcgc
    18601 acgcgcagtg tcctgaatac ctggcagttc aacaacgata cggtcagcac cctgacgctg
    18661 aaccaccggc tccgccacgc caagttggtt tacacggtta cgcaggatat taatgttctg
    18721 ctgcaccgca tattcacgcg cttcactcag acgagcatcg ctcattaccg cacgcagctg
    18781 gttgctgccc tggctgctaa tcaccaggtc cggatggcgc ttgctcagat acgcaatggc
    18841 ttcatcacga gctttagcat cgcggaaagt aatgctcagg ccgtagttgt tttctttacg
    18901 aacggtggta tacgggatgc ccttttcacg cagatcgctg cgcaggctat cgatattttg
    18961 ttcctggagt ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc
    19021 acgcaggtca aggccgagct tcatcggctc agcgtgaata gctgccagcc agcgcggcgt
    19081 tgccggggca aggttaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc
    19141 acgagcgcgc agctgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag
    19201 tgccacagac ttagcagtta ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt
    19261 ttgctcactg gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa
    19321 caggttggga agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt
    19381 ccacaaagga taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc
    19441 cttcatggtg cctttcggca ggacggcagc tacgaagtca cgtttaataa ctacttcagt
    19501 ggtgtcattc agcgcgatag caatgtagcc gttttccgct actttggtta cgcggccaac
    19561 caggccaccg ttcgtcagaa cttcatcacc tttggcaatg gagtccatca gctttttgtg
    19621 ttctttggtg cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac
    19681 caccagcatc aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac
    19741 cgcatcagaa ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca
    19801 aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac
    19861 cctcttcaat agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa
    19921 tggtgttgag tcgcgcgcct aatatttcgt tgcaacggtc aagatgatgc aagtaagcgc
    19981 gtgaataatt gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct
    20041 tatacttcgc attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg
    20101 cgttgcgggt tggcattacg cagtcaaaca tatcgatacc acgacgtacg ccttcaacca
    20161 ggtcttctgg tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcgggc
    20221 atacatgctc cagaatgcgg tgcatatctg ctttcggctc acccacagcc agaccgccga
    20281 cagcgtagcc atcaaaaccg atatctacca gacctttaac agaaatatca cgtaaatctt
    20341 cgtaaacgct gccctgaatg ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac
    20401 gctcacggct acgcttcgcc caacgcagag acatctccat ggagcgtttt gcgtaatccc
    20461 agtcagcagg atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt
    20521 actgaatctc cattgatttt tcaggatcga ggaaaatcgg atcgccgttg atcgggttac
    20581 ggaagtgcac gccctgttcg gtgattttac gaatatcgcc aaggctgaag acctggaaac
    20641 cgccggagtc ggtgaggatc ggccccttcc actgcataaa atcgtgcaga tcgccgtgca
    20701 gtttcatgat ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg aggataattt
    20761 gcgcgccagt ggcttcaact tcttccggcg tcatcccttt tacggtgccg taggtgccaa
    20821 caggcataaa acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg
    20881 cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca
    20941 gtccaacgtt ttaaaccagc gccgcggaat tactccccga cgcgctcatt aattgcctgc
    21001 ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt ctcttctacc
    21061 gctgctttat aggcgttcat ggtgtgttga taaccggcaa aggccgaaac cagcataatc
    21121 agcgtcgact ctggcaagtg gaagttcgtc accagcgcat cgaccacttt gtactggaag
    21181 cccggataga taaagatttg ggtatcgtcg aagaacggtt caatgagatc gtttttcgct
    21241 gcctgagccg cgctttccag cgaacgtact gaagtggtgc caaccgcaat cacccggtta
    21301 ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggtacttc agcgtattcc
    21361 gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca
    21421 acgtgcaacg tcacaaacgc catctccacg cctttggcgc gcaatttttc cagcaaaggc
    21481 tcgtcaaaat gcagaccagc ggtcggggct gcaaccgcgc ccggtttttc gctataaacg
    21541 gtttgataaa gttcgcggtc agcgtcttcg tccggacggt cgatatacgg cggcagcggc
    21601 atatggccga tactgttgag gatatccagt accgaacgat catcattgaa ttcgacttca
    21661 aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac tttcgtcatc gcccagcagc
    21721 agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg
    21781 tcgagcatcc gctcaaccag cacttcaatc ttgccgccgc tggctttacg cccaaacagg
    21841 cgcgccggga tcacgcgggt attattaaaa accagaagat cgccggggtt gagcttatca
    21901 agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa
    21961 cgacagctac tgcgttcagg catgggatag tgggcaatca gggattcggg caattcaaag
    22021 gagaaatcgg taacgcgcat gacactgact cagactaaaa taagaggcgg gtagtctagt
    22081 gccggggcgc tctccctgca acctttaccc ccttttggat agaatataat cgatgaattt
    22141 tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttccggca atttactggc
    22201 tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca
    22261 tatgcatcga cgtatcgacg tattaaccga caatctgccg gaagtccgcg aagcacggga
    22321 atggtttcgt agtgaaactc gccgcgttgc gcctattacg ctggatgtca tgtgggatca
    22381 ctttctttcc cgccactggt cgcagctgtc gccggacttt ccgctacagg aatttgtctg
    22441 ttatgcccgc gagcaagtga tgacgatttt gccggactca ccgccacgtt ttatcaatct
    22501 gaacaattac ttgtggtcag agcagtggct ggtgcgctat cgcgatatgg atttcatcca
    22561 gaacgtgtta aacggcatgg caagccgccg cccacgtctg gatgccctgc gtgactcctg
    22621 gtacgattta gacgctcatt atgacgccct cgaaacccgc ttctggcagt tttatccgcg
    22681 gatgatggcg caggcgtcac acaaggcgtt ataaaacgtt agttcatcca taccgtagcc
    22741 gaaatggcag gcaaagccag aatcccgttt gtcagttgcc catggccttc tttgcgttgc
    22801 cattgcgcga cattaagaaa cggtgacgcg gggagcacca cttcacaggc ctcgccacgg
    22861 ttgattgcca ccagtacacg ttgctgattc agcacgcgga caaataccac tacgttatct
    22921 tccgcataca gcacctgaca gccgccacga cgcagcgcct gacttttctt acgcagcgca
    22981 atcattcgct ggtacagcgc gaataacgcc gtatcctgct tttccacctg ccaggggaac
    23041 ggtttacggc aaaacggatc gtttttgcca tccagtccta cttcatcacc gtaataaatg
    23101 cacggtacac caggccaggt gaacagccag accaccgcca gcggcaggcg cgcaatatcc
    23161 cgaccgagca gcgttttaaa tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt
    23221 aattgttgtt gatgagaaag ccctgcgcgg taattatcca tccaggccat acaggtttgg
    23281 gcatcaattt gctgcggatc gtaagagata tcggtattgg caagaaatcc ccacaacggg
    23341 aatgtgaagc cacgatagtt catggcggca tcttccacat cggcctgtaa ccattgccgt
    23401 gcatcgccaa aatgttcgcc gacaatatac gcttccggct gggtttcttt cgccgcttcg
    23461 gtgatcccag caacgtgctg catattattg cgcgccccac ccgcctcccc cagcatatgc
    23521 accacatcca gccgccagcc gtccatactc cacggcgctt tcagccaatg gcggacaata
    23581 ctgtcttccc cgcgataaat ttcattcacc agactttccg actgataatc cagcttcggc
    23641 aagctggcat agccaagcca gtcgagcgcc gtgccatcat cactaaacga gtaccagtcg
    23701 cgccagggcg attcagggtt gtgacaagct ccccccgtgc cacgattgtg cctgtcaaac
    23761 caggcatggg aatcgccact gtggttaaac acgccatcca gcaccagccg cattcccagc
    23821 tgctgcgtat tgtggcgtaa gcgcagcaac gccccatcac cgccaaactg cggatcgaca
    23881 tggcgataat cctcggtatc gtatttatgt acgctgggag ctttaaacac cggattgaga
    23941 tacagcgccg tcacgccaag ctttttcaga tacggcagtt tttcgcttat cccgtccaga
    24001 tcgccgccat agaacgttga tccgcccgcc tgcgccgtga ccggttcatc ccagtcacgc
    24061 aagatgatct cttgtccggc tgcatgatgg taatagacat gatcctgttc agcttcacga
    24121 ggaagactac gcgcaaaacg atcagggaag atctgataaa aaatctgatc cgcagcccat
    24181 tgtgggccga tatccggtac atcgacggca aactgctcca gtcgtgccgg cggcattcgg
    24241 ctgaagccct gcggtgtaaa ccaacgctgg cgatcgtgcc acagcagttt gaaactgtaa
    24301 cgccgccggg gttgtccgct ggagagatca atcgccgcac gccatgcggt gacgccaggc
    24361 tgctgctgac tgcgctgctt atgcatcgat actgacattt cttcgttatc gtgttctgta
    24421 cgcagcataa tgcgctgcgg tgggtcttcg cccgtcagcc acagtgtaat gagcagttga
    24481 tctttgcttt gtttaacaaa tgggggcacc ggcaggtgcc atgcatttaa catcataatt
    24541 cccctgtgat gaaaatctga tcaccttgcc atagcctaat gcaggatcac tcatcatctg
    24601 aaggatattt gggggaggag gacgggggcg gataactttc ggaatagccg gatcaggatt
    24661 tcctcctgaa ccggcttaca gaacgcaagg attactggtt ttcagccagc tgacgatcgt
    24721 ggcggcgttt aaacatccag ccaatcaaca gcacaacaat ccacgcgaaa ccgacataca
    24781 gcgagatacg cgtatccggg tgataaccaa tcaacccgat aataaagagc aggaaaatca
    24841 aaccgccgat ggtcgttgct accccacccg gcactttaaa tttcagcgcc ttaacttctt
    24901 ctggcggcaa acggcgacgg aaggcaattt gcgacagcag gatcataatc cacacccaca
    24961 ccgtggcgaa ggttgccagc gaagcgatca ccaggaagac gttttccggc atgatgtagt
    25021 tcagatacac cgcaaacagc agcgcggtag tcatcaccag caccgtaacc cacggaatac
    25081 cgcgacgcga cgttttgctg aaaattttcg gcgcgctgcc ctgctctgcc ataccgtgga
    25141 gcatacggcc tacgccaaat acgtcactgt taattgccga cagcgaagca gtcagcacaa
    25201 caaagttaag aatgctggcg gcaaaggtaa tgcccatatg ctggaacgtc agcacgaacg
    25261 ggctaccggc agtgccaacc tgattccacg ggtagataga cataatgacg aacagcgtac
    25321 cgacgtagaa caccagaata cgcatcggca cggagttaat cgcacgcggt atcgatttct
    25381 caggatcttt cgcttcaccg gcggtaatcc cgataatttc gatcccaccg taagcaaaca
    25441 tcaccatttg caacgacatc accatcccaa gccagccgtt actgaagaag ccgccgttgc
    25501 tccacaggtt atgaataccg gtcggttgcc cgccgttgcc aatcccccag atgatgatgc
    25561 cgaaaccggc gacaatcatg atgatgatgg tggcgacttt aaagaacgag aaccagaatt
    25621 ccagctcacc gaagaccttc acgctcatca ggtttacggc gcaaatgatc agcaccacgc
    25681 tcagtaccca aatccagtgc ggcaccgtcg ggaaccagac acccatatag ataccaaaag
    25741 cggtcacatc ggcgatggcg acaataagga tttcaaagca ataggtccag ccggtaatgt
    25801 aacctgccag cgggccgagg ttttcctgcg cataacgcga gaaagagctg gctgccgggt
    25861 tatgtaccga catttccccc agcgcacgca taatgatata cgccgcgata ccaccgataa
    25921 tataggccaa caacacgctc ggaccggcca ttttgatggc gtctgccgaa ccgtaaaaca
    25981 gcccggtgcc aattgctgaa cccagtgcca taaagcgtat gtggcgggta cttagcccac
    26041 gctttagctt gttcttactt tccatcaatt cctaacccat caacacaata aaaaaccacg
    26101 gggcatcgca gccccgtggt taaaacaaat gttcagtgat ttagtgagcg ctggaggtca
    26161 cctgacgacc tgccgcgcga tcccagataa tggccagaac caccatcacc actgttggca
    26221 ttaaccacgc cagaccttgt tcggccagcg gtaaacgctg cgcccaggac ggtaagatat
    26281 cgctgaatgc agatgctttg atcccgtcga gaataccaaa aagcaggctg ataaacatcg
    26341 gcggagcaat cacgcgggac gaattatgcc accatgagcg tgtaaaactt aatacaacca
    26401 gtgcgataca cggcggataa atagcggtca gcaccggtac ggagatctgg atcagctggc
    26461 ttaagccgag gttagaaacc accatcgaga agccgccgag gataaacacc agcgtacgat
    26521 aagagagcgg tacgtactgg gcaaagaatt ctgcacaagc acaggtcagg ccaactgccg
    26581 ttaccaggca ggcgatgaag attaacgccg ccagcaggaa gctaccgccg ccgccaaagg
    26641 tgtgctgaac gtaagcatgc agaatagcag cgccgtttgc agactgatcg accagcgacg
    26701 cgctgtctga acccagacgg aacagcgcca ggtacagcag agtcagacca acacccgcca
    26761 tcaggccagc ccagacggta taacgggtca gcagacgcgc ttcggtaacg ccacgagaac
    26821 gcgccgcgtt aacaataacg ataccaaaca ccattgcgcc cagcgtatcc atggtcagat
    26881 agccgttaac gaagccgtta gaaaacgcag cgttttgata agcctcagtc gccgtgctga
    26941 tagaacccgc cggccagaca atcgcggcaa cagacaggat gaccagcgcg ataattttca
    27001 gcggcgcaag gaagttgccc acggtatcca gcagcttgcc cggatagagc gaaaccagaa
    27061 taacgatagc gaaatagacc aggctgtaga taaacagcgg cagcgcggaa tcaccagtca
    27121 gcggcgcaat ccccacttca aaggaaacgg tagctgtacg cggcgtagcg aaaagcggcc
    27181 ccaccgccag gtaacaaacc gttgccagca gtacgccagc gactttaccg attggcgtgc
    27241 tgaggctgtc aacaccgccg ccaacttttg ccagcgccac taccgttagt accggcagac
    27301 caacggcagt aatgaggaag ccgaatgccg cagtccagac gtgttcgcct gcctgcaagc
    27361 cgaccattgg agggaaaata atgttacctg cgccgacgaa caacgcaaat gtcataaagc
    27421 ccagagcgat gatatcgcgc gatcttaatt gatgggtcat aaatcatact gcctgtggat
    27481 gtggtgttgt aacgttaaat ttttgtgcca atccctgggg atgatacagc ggtatattta
    27541 aacaatttta gcgggaaata ctcccaacca ggcgcggcga agaatagttt ttcgatttac
    27601 cgcgttaatg cagtcagtat gacagcacct gaagcgcaat ttaaacgctt ataacgtttt
    27661 aaagcaagga gggatctaaa aaccagaggt atatagcgat aagaaatcac caaccagtat
    27721 aaaacactat ttccgtgaaa aagacatggc taatcatgcg aacaaaaaat caccaaccgt
    27781 tgtaaaatct aacgcccgta ttttgactgc aaagaaaata agccagcatc ggctggctta
    27841 tggcaataaa agatgacaaa ggcggattaa tcgctgtttt tggcaattaa acgttccggg
    27901 ataacaaaac tgaaacgtgt tccttttcct actgtactct caatattcag gcgactttcg
    27961 tgatgattca ccgcatgttt cacgatcgct aaccctaatc cgctaccgcc ggtttgccgg
    28021 gaacgcgctt tatcaacgcg ataaaaacgc tcggtcaggc gcggaatatg ctccggtgca
    28081 atgcccggtc cgttatcttc aacgctaaat tcggcaccgt gcggcactcg ctgccagcgt
    28141 acggtgatat gcgtgccttc tggagtatga ttcacggcgt tatagaccag gttcgaaatc
    28201 gcactgcgta gctgatcttc gttgccagac actttgaggc cgttatctat ctcaaaggta
    28261 aatgtctgtt ttttctgact cagagtctga gcctcacgct caacaacgcg cagcatcatc
    28321 ggcacatcaa ccttttcatt gagcaaatgc gtcggtgcgg cttctatttt cgacagcgtc
    28381 agcaactgct tcaccagccc ttccatccgc tgggtctgct cgcgcatggt gtgcaacgct
    28441 ttttcgcgta ccgcgccttc cagcggctgc tcatccatca tctccaggta accctgtaac
    28501 acggtcaatg gcgtacgtaa ctcatggctc acgttggcaa aaaagttacg ccgcgcccct
    28561 tccagttgat gcatttgcgt gacatcacgc gccaccatca gcaactgttt gtgggtataa
    28621 ggcatgacgc gaatttccag atgccgcccg gtgttgagca ccagattgag cgggcgagaa
    28681 aaatcacgcg ttttcagata ttgcgtaaac tccgggtaac gcagcaggtt aaggatgttc
    28741 tgcccgttat cttccggcca gcgcaaacca agaatttgtt gcgccagacc gttacaccag
    28801 aaaataccgc cctcttcagt ggtcagcacc actgcgtcgg gcagcgactc cgcgccgcta
    28861 cgaaagcgtt taatcagatt gcccagttca cggcggcgtt ttttatttcg cagctgcatc
    28921 tggtgtaagc cgtatagtag cggttcccag ctaccacgcc ccggtggcgg ggtcatacta
    28981 cgatccaccc acagccacca tgaaaggcgc aataaattcc agaaatgcca gataagcagt
    29041 cccgttaccg atgccagcaa aaaccagggc aggtaaccaa aaaatgcacc caggatgaaa
    29101 gccgggaagc agcaaagtag cagctccagc accagccttt tccacgacag ccgttccagc
    29161 acgtaagata ctccagttaa gaaatcataa gccctgctct gcgtccgatg agcaaggcgt
    29221 taaaagcggg ttgaaaaacg atatcctgta ccgcgcacgg tctgcaccat gcggtcatgc
    29281 ccgcctggct ccagtgcttt acgtaggcga cgaatgtgga catcgaccgt acgatcttcc
    29341 acatacacgt tagttcccca gacgtggttt aacagctgct cgcggctgta cacgcgctca
    29401 ggatgcgtca taaaaaagtg cagcagttta aattctgtcg gccccatctc cagcggctct
    29461 tcgcccgcca tcactcggtg agatgtcggg tcgagactta atccctgcat ctcaatcacc
    29521 tcttccaccg ccattggcga aatacggcgc attaccgctt tgattcgcgc caccagctcc
    29581 ttcggcgaaa acggcttggt gatatagtca tccgcgccgg tttcaaggcc gcgcacgcga
    29641 tcttcttctt cccctctggc ggtcaacatc accactggaa tatcccgggt catcgactcg
    29701 cgcttgaggt gtttgatgaa ctggataccg gagccgccag gtaacatcca gtcaaggaga
    29761 attaaatccg gccagggttc attcagttga ttcacagcac tgtcataatc ttccgcttcg
    29821 accggctgaa agccattttg ttcgagcacg aagcagacca tttcgcgaat tggagcttca
    29881 tcttctacga ccagaatacg tctcgccatg atttgccctg ttgtaataaa taggttgcga
    29941 tcattaatgc gacgtcatta tgcgtcagat ttatgacaga tttatgaaaa gctcgtcgca
    30001 catatcttca ggttattgat ttccgtggcg cagaaaaaag caaatggcac atctgtttgg
    30061 gtataatcgc gcccatgctt tttcgccagg gaatcgttat gcgcatcctt cacacctcag
    30121 actggcatct cggccagaac ttctacagta aaagccgcga agctgaacat caggcttttc
    30181 ttgactggct gctggaaaca gcacaaaccc atcaggtgga tgcgattatt gttgctggtg
    30241 atgttttcga taccggctcg ccgcccagtt acgcccgcac gttatacaac cgttttgttg
    30301 tcaatttaca gcaaactggc tgtcatctgg tggtactggc aggaaaccat gactcggtcg
    30361 ccacgctgaa tgaatcgcgc gatatcatgg cgttcctcaa tacgaccgtg gtcgccagcg
    30421 ccggacatgc gccgcaaatc ttgcctcgtc gcgacgggac gccaggcgca gtgctgtgcc
    30481 ccattccgtt tttacgtccg cgtgacatta ttaccagcca ggcggggctt aacggtattg
    30541 aaaaacagca gcatttactg gcagcgatta ccgattatta ccaacaacac tatgccgatg
    30601 cctgcaaact gcgcggcgat cagcctctgc ccatcatcgc cacgggacac ttaacgaccg
    30661 tgggtgccag taaaagtgac gccgtgcgtg acatttatat tggcacgctg gacgcgtttc
    30721 cagcacaaaa ctttccacca gccgactaca tcgcgctcgg gcatattcac cgcgcacaga
    30781 ttattggcgg catggaacat gttcgctatt gcggctctcc cattccactg agttttgatg
    30841 aatgcggaaa gagtaaatat gtccatctgg tgacattttc aaacggcaaa ttagagagcg
    30901 tggaaaacct gaacgtaccg gtaacgcaac ccatggcggt gctgaaaggc gatctggcgt
    30961 cgattaccgc acagctggaa cagtggcgcg atgtatcaca ggagccgcct gtctggctgg
    31021 atatcgaaat cactgctgat gagtatctgc atgatattca gcgcaaaatc caggcattaa
    31081 ccgaatcatt gcctgttgaa gtattgctgg tacgccggag tcgtgaacag cgcgagcgtg
    31141 tgttagccag ccaacagcgt gaaaccctca gcgaactcag cgtcgaagag gtgttcaatc
    31201 gccgtctggc actggaagaa ctggatgaat cgcagcagca acgtctgcag catcttttca
    31261 ccacgacgtt gcataccctc gccggagaac acgaagcatg aaaattctca gcctgcgcct
    31321 gaaaaacctg aactcattaa aaggcgaatg gaagattgat ttcacccgcg agccgttcgc
    31381 cagcaacggg ctgtttgcca ttaccggccc aaccggtgcg gggaaaacca ccctgctgga
    31441 cgccatttgt ctggcgctgt atcacgaaac tccgcgtctc tctaacgttt cacaatcgca
    31501 aaatgatctc atgacccgcg ataccgctga atgtctggcg gaggtggagt ttgaagtgaa
    31561 aggtgaagcg taccgtgcat tctggagcca gaatcgggcg cgtaaccaac ccgacggtaa
    31621 tttgcaggtg ccacgcgttg agctggcgcg ctgcgccgac ggcaaaattc tcgccgacaa
    31681 agtgaaagat aagctagaac tgacagcaac gttaaccggg ctggattacg ggcgcttcac
    31741 ccgttcgatg ctgctttcgc aggggcaatt tgctgccttc ctgaatgcca aacccaaaga
    31801 acgcgcggaa ttgctcgagg agttaaccgg cactgaaatc tacgggcaaa tctcggcgat
    31861 ggtttttgag cagcacaaat cggcccgcac tgagctggag aagctgcaag cgcaggccag
    31921 cggcgtcgcg ttgctcacgc cggaacaagt gcaatcgctg acagcgagtt tgcaggtact
    31981 tactgacgaa gaaaaacagt tacttaccgc gcagcagcaa gaacaacaat cactaaactg
    32041 gttaacgcgt ctggacgaat tgcagcaaga agccagccgc cgtcagcagg ccttgcaaca
    32101 ggcgttagcc gaagaagaaa aagcgcaacc tcaactggcg gcgctcagtc tggcacaacc
    32161 ggcacgaaat cttcgtccgc actgggaacg catcgcagaa cacagcgcgg cgctggcgca
    32221 tactcgccag cagattgaag aagtaaatac tcgcttacag agcacaatgg cgcttcgcgc
    32281 gagcattcgc caccacgcgg cgaagcagtc agcagaatta cagcagcagc aacaaagcct
    32341 gaatacctgg ttacaggaac acgaccgctt ccgtcagtgg aacaacgaac tggcgggttg
    32401 gcgtgcgcag ttctcccaac aaaccagcga tcgcgagcat ctgcggcaat ggcagcaaca
    32461 gttaacccat gctgagcaaa aacttaatgc gcttgcggcg atcacgttga cgttaaccgc
    32521 cgatgaagtt gctaccgccc tggcgcaaca tgctgagcaa cgcccactgc gtcagcacct
    32581 ggtcgcgcta catggacaga ttgttcccca acaaaaacgt ctggcgcagt tacaggtcgc
    32641 tatccagaat gtcacgcaag aacagacgca acgtaacgcc gcacttaacg aaatgcgcca
    32701 gcgttataaa gaaaagacgc agcaacttgc cgatgtgaaa accatttgcg agcaggaagc
    32761 gcgcatcaaa acgctggaag ctcaacgtgc acagttacag gcgggtcagc cttgtccact
    32821 ttgtggttcc accagccacc cggcggtcga ggcgtatcag gcgctggagc ctggtgttaa
    32881 tcagtctcga ttactggcgc tggaaaacga agttaaaaag ctcggcgaag aaggtgcggc
    32941 gctgcgcggg caactggacg ccttaacaaa gcagcttcag cgcgatgaaa acgaagcgca
    33001 aagcctccga caagatgagc aagcacttac tcaacaatgg caagccgtca cggccagcct
    33061 caatatcacc ttgcagccac aggacgatat tcaaccgtgg ctggatgcac aagatgagca
    33121 cgaacgccag ctgcggttac tcagccaacg acatgaatta caagggcaga ttgccgcgca
    33181 taatcagcaa attatccagt atcaacagca aattgaacaa cgccagcagc agctcttaac
    33241 ggcattggcg ggttatgcac tgacattgcc acaggaagat gaagaagaga gctggttggc
    33301 gacacgtcag caagaagcgc agagctggca gcaacgccag aacgaattaa ccgcgctgca
    33361 aaaccgtatt cagcagctga cgccgattct ggaaacgttg ccgcaaagtg atgatctccc
    33421 gcacagcgaa gaaactgtgg cgctggataa ctggcggcag gttcatgaac aatgtctcgc
    33481 attacacagc cagcagcaga cgttacagca acaggatgtt ctggcggcgc aaagtctgca
    33541 aaaagcccag gcgcagtttg acaccgcgct acaggccagc gtctttgacg atcagcaggc
    33601 gttccttgcg gcgctaatgg atgaacaaac actaacgcag ctggaacagc tcaagcagaa
    33661 tctggaaaac cagcgccgtc aggcgcaaac gctggtcact cagacagcag aaacgctggc
    33721 acagcatcaa caacaccgac ctgacgggtt ggctctcact gtgacggtgg agcagattca
    33781 gcaagagtta gcgcaaactc accaaaagtt gcgtgaaaac accacgagtc aaggcgagat
    33841 tcgccagcag ctgaagcagg atgcagataa ccgtcagcaa caacaaacct taatgcagca
    33901 aattgctcaa atgacgcagc aggttgagga ctggggatat ctgaattcgc taataggttc
    33961 caaagagggc gataaattcc gcaagtttgc ccaggggctg acgctggata atttagtcca
    34021 tctcgctaat cagcaactta cccggctgca cgggcgctat ctgttacagc gcaaagccag
    34081 cgaggcgctg gaagtcgagg ttgttgatac ctggcaggca gatgcggtac gcgatacccg
    34141 taccctttcc ggcggcgaaa gtttcctcgt tagtctggcg ctggcgctgg cgctttcgga
    34201 tctggtcagc cataaaacac gtattgactc gctgttcctt gatgaaggtt ttggcacgct
    34261 ggatagcgaa acgctggata ccgcccttga tgcgctggat gccctgaacg ccagtggcaa
    34321 aaccatcggt gtgattagcc acgtagaagc gatgaaagag cgtattccgg tgcagatcaa
    34381 agtgaaaaag atcaacggcc tgggctacag caaactggaa agtacgtttg cagtgaaata
    34441 actattcagc aggataatga atacagaggg gcgaattatc tcttggcctt gctggtcgtt
    34501 atcctgcaag ctatcacttt attggctacg gtgattggta gccgttctgg tggttgtgat
    34561 ggtggtatga aaaaagtcat tttatctttg gctctgggca cgtttggttt ggggatggcc
    34621 gaatttggca ttatgggcgt gctcacggag ctggcgcata acgtaggaat ttcgattcct
    34681 gctgccgggc atatgatctc gtattatgca ctgggggtgg tggtcggtgc gccaatcatc
    34741 gcactctttt ccagccgcta ctcactcaaa catatcttgt tgtttctggt ggcgttgtgc
    34801 gtcattggca acgccatgtt cacgctctct tcgtcttacc tgatgcttgc cattggtcgc
    34861 ctggtttccg gctttccgca tggcgcattt tttggcgtcg gcgcgatcgt gttatcaaaa
    34921 attatcaaac ccggaaaagt caccgccgcc gtggcgggga tggtttccgg gatgacagtc
    34981 gccaatttgc tgggcattcc gctgggaacg tatttaagtc aggaatttag ctggcgttac
    35041 acctttttat tgatcgctgt ttttaatatt gcggtgatgg catcggtcta tttttgggtg
    35101 ccggatattc gcgacgaggc gaaaggaaag ctgcgcgaac aatttcactt tttacgcagc
    35161 ccggccccgt ggttaatttt cgccgccacg atgtttggca acgcaggtgt gtttgcctgg
    35221 ttcagctacg taaagccata catgatgttt atttccggtt tttcggaaac ggcgatgacc
    35281 tttattatga tgttagttgg gctagggatg gtgctgggga atgtgctaag tggcaggatt
    35341 tcaggacgtt attcaccact gcgcattgca gcagtaactg actttatcat tgtactggca
    35401 ctgctgatgc tttttttctg cggcggcatg aaaacaacgt cgcttatttt tgcttttatt
    35461 tgttgcgcgg gattatttgc cctttcagca cctctgcaaa tattgttact acaaaacgcc
    35521 aaaggcggag agttattagg tgccgcaggt gggcaaatag cgtttaacct cggtagcgcc
    35581 gtcggcgcat attgcggagg tatgatgctg acgctggggc tggcatataa ttacgtggcg
    35641 ctgcctgccg ccctgctttc gtttgctgcg atgtcatcgt tgctgctgta tggtcgctat
    35701 aagcgccagc aagcggcgga tagtccggtg ctggcgaaac cactggggta gattactgtc
    35761 acggtgcgcg ttgcttattg ccggatgcgg cgtgaacgcc ttatccgccc tacgcggttc
    35821 cggcacattt tgtagatact gataagacgc ggcaagcgtc gcatcaagca tgatgcgcca
    35881 attgcctacg ttttttactc ttgtggccat aaccacgcag cgccgcgtac gccgctggaa
    35941 tcaccgtgct tcgccttacg caccggcgtt tcacattcgc cgccgaagac aaattgttta
    36001 atcaactgcc caaccgtttg atataaacgg tctacattgc tcatcccgcc ccccaggaca
    36061 atcacatccg gatcgagaat attcacgaca tgtgccagcg attttgccag ccgcagctcg
    36121 tagcgacgca atgccagttc cgctaccgga tcgctttctt caaccaggcg gataatttca
    36181 ctgcctttca gcgcatgtcc gctcaaacga cgataatctg tggcaaatcc cgtgcccgaa
    36241 ataaaggttt caatacaacc ttgtttaccg caataacaag ggacttcctc gcgataacgc
    36301 agttcgtctt cgtccatcca cggtagcgga ttgtgtcccc actcgcctgc cgtgccattg
    36361 ccgccgatat gcgcccgccc attgaatgcc acgcccgcgc cgcatcccgt gccgataatc
    36421 acggcaaata ccgtctgtgc tcccgctgcc gcgccatcta ctgcttctga aacggccaga
    36481 cagttggcgt catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa gtctttatcg
    36541 aatggctgac cgttgagcca ggttgaattg gcattcttaa ccacaccggt gtaaggcgaa
    36601 attgagccag gaatgcccat acctaccgtt ccgcgctgcc ctgtcgcctg ctccgccata
    36661 tcaaccaacg tggcgatcgt ttcaatagtc tgccggtaat catcacgcgg cgtgggcaga
    36721 cgatggcggt acaactgctc ccctgcatcg cccagtgcaa tcacttcagt tttggtgccg
    36781 cctaaatcga tacctatacg cacggtactc tccttatttt tttcaatatc aatagcgtag
    36841 agacggacaa ccggattggc aatgcaaggc cgccgacaat tcgttatcat gcccgctaaa
    36901 tttaacgaca aggccgtgga aattatcatg ctgtggttca aaaatttaat ggtttaccgt
    36961 cttagccgcg agatttcgct gcgtgcagaa gagatggaaa aacagctagc ctcgatggca
    37021 tttaccccat gcggcagcca ggacatggcg aagatgggct gggttcctcc gatgggatcg
    37081 cacagcgatg cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc gcgcaaagaa
    37141 gaaaaaatcc tcccgtctcc ggtgattaag caggcgctgg aagcgaaaat cgccaaactg
    37201 gaagcggaac aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa agacgaagtg
    37261 ctgcactctc tgttgccgcg tgctttcagc cgttttagcc agacaatgat gtggatcgac
    37321 acggttaacg gtttgattat ggtggactgc gccagtgcca aaaaagcgga agatacgctg
    37381 gcactgctgc gtaaaagcct ggggtcgtta ccggttgtac cattgagcat ggaaaacccg
    37441 attgaactga cgttgaccga atgggttcgc tccggtagtg cggcacaggg cttccagctg
    37501 cttgatgaag ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa
    37561 caagatctga ccagcgaaga gatcaccaat cacattgaag ccggaaaagt ggtgactaaa
    37621 ctggcgctcg actggcagca gcgcattcag tttgtgatgt gcgacgatgg ttcgctcaag
    37681 cgtctgaagt tctgcgacga gctgcgcgat caaaacgaag atatcgaccg tgaagatttc
    37741 gcccagcgtt ttgatgccga tttcatcctg atgactggtg aactggcagc gttaattcaa
    37801 aacctgattg aaggattagg tggcgaagca caacgttaat tgctgatttt cctttaatgc
    37861 cggatgcgac gcctgccgcg tcttatccgg cgtacgaagc cacaccaggc atataattat
    37921 tcgctacggc gggcaataat ttttagcgca gcaatattat gcgttttatg ctgtaacttg
    37981 ctccatggac gttgtgtcat tgtttttcct caagccagtg acgcgcttca ctgcgaattt
    38041 tccgcattgc cccggttttc actaaatccc gtaacagcga aagatcgggt tgttcgacaa
    38101 ataagagtaa tgatggatcc tcttttaacc acgtcgtttt cacatcggca gcgagctgtg
    38161 gattattcat agctagcgac cgatgccgag actcggtcaa tgtcgttaac aacgacgccg
    38221 gggtatgtgc gttgcgtaat gccgtttggc gcaaatgatc attgcgactt tgcagcaatg
    38281 cacgccacca ttcatcatct gatttatctt ttatcgtagc cagttccgca acaatatctg
    38341 gagatcttca ctgatattta tatcaacctt gatgaattat gaatataccg gaaattgaaa
    38401 aaatcgcagc aaaggaaata ttattatggg ttaaagagag gatatttcag aaaaatgccc
    38461 tcccgttcaa ggggaaggcg aggaattaca gatagcggca cagataagag gtgggttcgg
    38521 caacttgcag atgaaactca ctgtgaccgg gaacattaaa caccgaaccg gcttcataca
    38581 cctgccagtc ggtcgcgtca ggcagtaaca cattcagcgc gccactgatt accgtcattt
    38641 cttccggctc agcggtgctg aaggtgtatt cgccttcaac cataacaccc acgctggcgc
    38701 gaccagtgct gctgctggaa aagccgattg atttcacttt gccggaaaag tactcattac
    38761 tttgaagcat aaactggccc tgtttggtag ttgaacatct acttcactat aggggccaga
    38821 ggcgctggct gtcacgcaaa attacaccag taattccgca gccagccgtg caatcaatac
    38881 gttagacagc aagacaggaa catcgagctg tttttgcagt aaatcgcgat gacgttggtg
    38941 aaatcctaaa caatccagca tgatgacatc agcccctttt gccagtaatt ctttcccggc
    39001 atcaatgatt ttttgttctg aatcatgaat ggggttaccc aatgaaaata ccggcgattt
    39061 ctgcaaaatt tgccattttt gcgcctgaac gggcagcatc tcctcaaccg gaacgataac
    39121 ccccacctga tgatcttcaa caatagagga aaccagtgga ggcaatattc gcgacggctc
    39181 aagaaagatc gtattacgcg cagtcatact actaatgttt gctgtactca ttaataaaat
    39241 gacgtcataa ccctgattat cgagcacttc aaccacacct tgcaggtctc gctccacttt
    39301 acgacgcgaa acatgggcca gctggatgtc atttaataat gtgagaatgg tgtcttcgcc
    39361 tgcttctggc gcgtactccg ccatcacttc ttcacgactt aacttcccca gcaggctatg
    39421 atgggaaata ttatcttcgt caatgtattc cgtcaggagc ggcaaaacct cctgcatagg
    39481 tacaatgccg atggtcagga tcgccaacga cgcactcatt gttcatccac cttacttttc
    39541 tttcgtcgtt aattaccggg caagtgtgaa gcaccactgc tgacacttac ttgtgctaca
    39601 aatgacaaaa agcgtagcag cagacgtgca cggcataatg taaagattcc aaatgattcc
    39661 agtaatggat tgttattgtt taatattcta attattagaa aaacatgaat tatgaaaaat
    39721 gtgacgcaga tcccttgtag tggcactcag aatcccttcc gagcagtctg atgcggtcac
    39781 tctttgtcag ggtcctcata gcgttttttc gcatccatcg cttcctgagc cgtcggatgt
    39841 tcactgatca acgagtctgg tttaggatga tcggctctga gttggtacca ggttaccgtc
    39901 tgtcctggct ttcctttttc aatcgtcact atatatgctt cacgaggata aggcggtttc
    39961 gttggcatcg ttcttcctta tttcacggga tgaacgttaa gtataggcgc tctaaaatca
    40021 acaattgatc gtctgtgcca gggcgctacg aatttcagaa atcacctggc tgggttcgtt
    40081 tgttgcgtcg atgataatat gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac
    40141 ttcctgaact tcttcgctca gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg
    40201 tgcagcttgc agtcggttaa ccaggactga tactggcgca cacaaataaa ccacgatccc
    40261 gttattttgc ataaagtgac gattaaattc cgtcagaata atgccgccgc ctgtagcgat
    40321 aacggtggat ggcgcagtta ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc
    40381 ccactcttcc ctttcgacga tctccgcgac cgtcatattg agctgtgatt gcaaccactg
    40441 atcggtatcg acaaaccgac ggttaagcga atcggcaagg gccattccga ccgttgtttt
    40501 accacagccc cgaggcccga tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata
    40561 ggtcgcaatg cggcgaaagc cggtgtcatg agaatagcga tcataccatc aaactagtac
    40621 aatttcgatt gtaaagaaaa aattccactt aaagtga
//
LOCUS       DAZFWP020000022        40297 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFWP020000022 DAZFWP020000000
VERSION     DAZFWP020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.022"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHK0Z_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHK0Z_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU4501135.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            727..2925
                     /gene="priA"
                     /locus_tag="ACHK0Z_RS05370"
                     /old_locus_tag="pgaptmp_001074"
     CDS             727..2925
                     /gene="priA"
                     /locus_tag="ACHK0Z_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU4501136.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKEQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3081..4106
                     /gene="cytR"
                     /locus_tag="ACHK0Z_RS05375"
                     /old_locus_tag="pgaptmp_001075"
     CDS             3081..4106
                     /gene="cytR"
                     /locus_tag="ACHK0Z_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU4501137.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHK0Z_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHK0Z_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU4501138.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQADTVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5250..5780
                     /gene="hslV"
                     /locus_tag="ACHK0Z_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             5250..5780
                     /gene="hslV"
                     /locus_tag="ACHK0Z_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU4501139.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5790..7121
                     /gene="hslU"
                     /locus_tag="ACHK0Z_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             5790..7121
                     /gene="hslU"
                     /locus_tag="ACHK0Z_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU4501140.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7188..8114
                     /gene="menA"
                     /locus_tag="ACHK0Z_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             7188..8114
                     /gene="menA"
                     /locus_tag="ACHK0Z_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU4501141.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8207..8692
                     /gene="rraA"
                     /locus_tag="ACHK0Z_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             8207..8692
                     /gene="rraA"
                     /locus_tag="ACHK0Z_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU4501142.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHK0Z_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHK0Z_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU4501143.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9447..10292
                     /gene="glpF"
                     /locus_tag="ACHK0Z_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             9447..10292
                     /gene="glpF"
                     /locus_tag="ACHK0Z_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU4501144.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10315..11823
                     /gene="glpK"
                     /locus_tag="ACHK0Z_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             10315..11823
                     /gene="glpK"
                     /locus_tag="ACHK0Z_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU4501145.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11959..12969
                     /gene="glpX"
                     /locus_tag="ACHK0Z_RS05420"
                     /old_locus_tag="pgaptmp_001084"
     CDS             11959..12969
                     /gene="glpX"
                     /locus_tag="ACHK0Z_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4501146.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13066..13812
                     /gene="fpr"
                     /locus_tag="ACHK0Z_RS05425"
                     /old_locus_tag="pgaptmp_001085"
     CDS             13066..13812
                     /gene="fpr"
                     /locus_tag="ACHK0Z_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU4501147.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="ACHK0Z_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     CDS             complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="ACHK0Z_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU4501148.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14272..14571)
                     /gene="yiiS"
                     /locus_tag="ACHK0Z_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     CDS             complement(14272..14571)
                     /gene="yiiS"
                     /locus_tag="ACHK0Z_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU4501149.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14783..15223)
                     /gene="yiiR"
                     /locus_tag="ACHK0Z_RS05440"
                     /old_locus_tag="pgaptmp_001088"
     CDS             complement(14783..15223)
                     /gene="yiiR"
                     /locus_tag="ACHK0Z_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4501150.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15324..15923
                     /gene="yiiQ"
                     /locus_tag="ACHK0Z_RS05445"
                     /old_locus_tag="pgaptmp_001089"
     CDS             15324..15923
                     /gene="yiiQ"
                     /locus_tag="ACHK0Z_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU4501151.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16031..16798
                     /gene="tpiA"
                     /locus_tag="ACHK0Z_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     CDS             16031..16798
                     /gene="tpiA"
                     /locus_tag="ACHK0Z_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU4501152.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="ACHK0Z_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     CDS             complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="ACHK0Z_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU4501153.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="ACHK0Z_RS05460"
                     /old_locus_tag="pgaptmp_001092"
     CDS             complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="ACHK0Z_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU4501154.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="ACHK0Z_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     CDS             complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="ACHK0Z_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU4501155.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="ACHK0Z_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="ACHK0Z_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU4501156.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21218..21718)
                     /gene="cpxP"
                     /locus_tag="ACHK0Z_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             complement(21218..21718)
                     /gene="cpxP"
                     /locus_tag="ACHK0Z_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU4501157.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21868..22566
                     /gene="cpxR"
                     /locus_tag="ACHK0Z_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             21868..22566
                     /gene="cpxR"
                     /locus_tag="ACHK0Z_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU4501158.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22563..23936
                     /gene="cpxA"
                     /locus_tag="ACHK0Z_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             22563..23936
                     /gene="cpxA"
                     /locus_tag="ACHK0Z_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU4501159.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24042..24716)
                     /gene="yiiM"
                     /locus_tag="ACHK0Z_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             complement(24042..24716)
                     /gene="yiiM"
                     /locus_tag="ACHK0Z_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU4501160.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24865..25848)
                     /gene="kdgT"
                     /locus_tag="ACHK0Z_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             complement(24865..25848)
                     /gene="kdgT"
                     /locus_tag="ACHK0Z_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU4501161.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26108..26728)
                     /gene="sodA"
                     /locus_tag="ACHK0Z_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             complement(26108..26728)
                     /gene="sodA"
                     /locus_tag="ACHK0Z_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU4501162.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27013..28047
                     /gene="rhaT"
                     /locus_tag="ACHK0Z_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             27013..28047
                     /gene="rhaT"
                     /locus_tag="ACHK0Z_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGU4501163.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGLSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28044..28892)
                     /gene="rhaR"
                     /locus_tag="ACHK0Z_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             complement(28044..28892)
                     /gene="rhaR"
                     /locus_tag="ACHK0Z_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU4501164.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28966..29802)
                     /gene="rhaS"
                     /locus_tag="ACHK0Z_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             complement(28966..29802)
                     /gene="rhaS"
                     /locus_tag="ACHK0Z_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU4501165.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30090..31559
                     /gene="rhaB"
                     /locus_tag="ACHK0Z_RS05520"
                     /old_locus_tag="pgaptmp_001104"
     CDS             30090..31559
                     /gene="rhaB"
                     /locus_tag="ACHK0Z_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU4501166.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31556..32815
                     /gene="rhaA"
                     /locus_tag="ACHK0Z_RS05525"
                     /old_locus_tag="pgaptmp_001105"
     CDS             31556..32815
                     /gene="rhaA"
                     /locus_tag="ACHK0Z_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU4501167.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33057..33881
                     /gene="rhaD"
                     /locus_tag="ACHK0Z_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             33057..33881
                     /gene="rhaD"
                     /locus_tag="ACHK0Z_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU4501168.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33891..34205
                     /gene="rhaM"
                     /locus_tag="ACHK0Z_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             33891..34205
                     /gene="rhaM"
                     /locus_tag="ACHK0Z_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU4501169.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34506..34952
                     /gene="frvA"
                     /locus_tag="ACHK0Z_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             34506..34952
                     /gene="frvA"
                     /locus_tag="ACHK0Z_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU4501170.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            34963..36414
                     /gene="frvB"
                     /locus_tag="ACHK0Z_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             34963..36414
                     /gene="frvB"
                     /locus_tag="ACHK0Z_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU4501171.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36404..37474
                     /gene="frvX"
                     /locus_tag="ACHK0Z_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             36404..37474
                     /gene="frvX"
                     /locus_tag="ACHK0Z_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4501172.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37474..39222
                     /gene="frvR"
                     /locus_tag="ACHK0Z_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             37474..39222
                     /gene="frvR"
                     /locus_tag="ACHK0Z_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU4501173.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39272..>40297)
                     /gene="yiiG"
                     /locus_tag="ACHK0Z_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             complement(39272..>40297)
                     /gene="yiiG"
                     /locus_tag="ACHK0Z_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU4501174.1"
                     /translation="LSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVA
                     KAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTG
                     EERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMD
                     KYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEE
                     REGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNF
                     SFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMV
                     DDYNSLR"
BASE COUNT         9781 a        10460 c        10426 g         9630 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcaccccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccga tgcgcacccc ggctgaaccc tggacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa
      181 ccagaagcct agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt
      241 tcagaattta taaaaggcaa attacaggca caaaaaaagc gccgtgcggc gcttttttcg
      301 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
      361 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
      421 tcgaggttca ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag
      481 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
      541 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
      601 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa
      661 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
      721 gatgctatgc ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
      781 ctgctgccag aaggcatgac ggttaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa
      841 gagcaggagc gcatcggggt tgtggtgtca gttagcgatg tcagtgagct gccgcttaac
      901 gagctgaaag cggtagtgga agtgctggac gttgagccgg tatttactca ctccgtctgg
      961 cggttactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     1021 gccttgccga ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     1081 tttgccactg aacaaggcca ggcggtggat ctgaacagcc tgaaacgctc ccccaagcaa
     1141 caacaggcgc tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgccacgctc
     1201 gaatttaatg atgccgcgtt gcaggcgcta cgcaaaaaag gtctgtgtga tttagcaagt
     1261 gaaacaccag agtttagcga ctggcaaacg aactatgccg tttctggtga gcggttgcga
     1321 ttgaataccg aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct
     1381 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     1441 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
     1501 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc
     1561 ggcctgaacg acagcgagcg gctttcggcg tggttgaaag cgaaaaatgg cgaggcggcg
     1621 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     1681 attgatgaag agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc
     1741 gacctggcgg tgtatcgtgc gcatagcgag caaatcccga ttattcttgg ctccgcaacg
     1801 cccgcgctgg aaacgttgtg caacgtccag cagaaaaaat accgcctgct gcgcctgacg
     1861 cgccgggcgg ggaatgcgcg tccggcaatt caacacgtgc tggatttaaa aggtcagaag
     1921 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     1981 aatcaggtta tcctctttct taatcgtcgg ggttttgcgc ctgcgctact gtgccacgac
     2041 tgcggctgga ttgccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     2101 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     2161 tgcggttcca cgcacctggt acccgtgggg ctgggcaccg aacagcttga acagacgctt
     2221 gcgccgctgt tccctgatgt gcccatttct cgtatcgacc gcgataccac cagccgcaaa
     2281 ggggcgctgg aacagcaact ggcggaagta catcgcggcg gtgcgaggat tttgatcggc
     2341 acacaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     2401 gtggacggcg cgctgttttc tgccgatttt cgctcggcag agcgtttcgc tcagctttac
     2461 acccaggtcg ctggtcgtgc cgggcgtgcg ggtaaacaag gcgaagtggt gctgcaaacg
     2521 caccatccag aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc
     2581 gaacaggcgc tggctgagcg acggatgatg caactgccgc catggaccag ccatgtgatt
     2641 gtgcgtgcgg aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat
     2701 ctgatcctct ccagcccgct ggcagacgac aaactgtggg ttctcggtcc ggttccggca
     2761 ctggcaccta aacgtggcgg tcgctggcgc tggcagatat tgttgcagca cccttcccgc
     2821 gtgcgcttgc aacacatcat tagtggtacg ctggcgctca tcaatacaat accggattcc
     2881 cgcaaggtga aatgggtact ggatgttgat ccgattgagg gttaaaccgc ttacgatgcg
     2941 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     3001 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     3061 gtgccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     3121 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     3181 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     3241 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     3301 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     3361 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     3421 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     3481 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     3541 aattcgcacc ggaactggag ctacctacag ttcatatcga caatctgacc gccgcatttg
     3601 atgcagtaaa ttatctatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     3661 aagagatgcc gctgtgtcac taccgcctgc aaggttacgt tcaggcgctg cgtcgctgcg
     3721 gcattatggt tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     3781 aagcgatgca gcagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg
     3841 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
     3901 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     3961 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     4021 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     4081 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     4141 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     4201 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     4261 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     4321 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag
     4381 tccgagacgc tgcaaagcca gaaagtgacc ggtaacggac taccaccaaa accagaagaa
     4441 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
     4501 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
     4561 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga agttccgtgg
     4621 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
     4681 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
     4741 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct
     4801 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
     4861 aagccgcagc aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     4921 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     4981 gatacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
     5041 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     5101 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     5161 cctcaaaatc ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta
     5221 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     5281 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     5341 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     5401 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     5461 atctggtcaa agccgccgtt gagctggcga aagactggcg taccgatcgc atgctgcgca
     5521 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     5581 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     5641 aggctgcggc gcgtgcactg ttagaaaata ctgaactgag tgctcgtgaa attgctgaaa
     5701 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     5761 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     5821 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     5881 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     5941 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     6001 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct
     6061 acgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     6121 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     6181 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     6241 aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
     6301 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag
     6361 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     6421 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     6481 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
     6541 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     6601 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     6661 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     6721 agattgcgaa accgtctgac ctgatcccgg aactgcaagg tcgtctgcca atccgcgttg
     6781 aactgcaggc gctgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     6841 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     6901 ccggtattaa gcgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
     6961 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
     7021 gcgatttaag cggtcaaact atcatcattg acgcagatta tgtgagcaaa catctggatg
     7081 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     7141 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     7201 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     7261 ttgctgcaat tatcgtcggg acagcgctgg catggtggca aggtcacttc gatccgctgg
     7321 tcgccctgct ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg
     7381 attacggcga tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca
     7441 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     7501 tcatctgtct ctccgggctg gcactggttg cagtggcatg ccatacgctg gccgattttg
     7561 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     7621 atcgtcctta tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt
     7681 tgagtgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
     7741 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa tattaataac ctgcgtgata
     7801 tcaatagcga ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     7861 cgcgtcgtta tcatgcctgc ctgctgatgg gctcgctggt gtgtctggcg ctgtttaatc
     7921 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga
     7981 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac
     8041 gtactgtcaa gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc
     8101 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
     8161 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
     8221 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
     8281 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
     8341 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
     8401 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
     8461 tgaatgggaa ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
     8521 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
     8581 cgaaagcgat gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta
     8641 tgccgacaat accgggatta ttctttctga agatccgctg gatattgaat gatagaaaag
     8701 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
     8761 ggaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
     8821 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     8881 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     8941 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     9001 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaatc
     9061 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
     9121 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact
     9181 tttcatcctt ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc
     9241 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg
     9301 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgtttt tgctcgttaa
     9361 cgataagttt acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
     9421 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
     9481 ttgctgaatt cctcggtacc gggttgttga ttttcttcgg tgtgggttgc gttgcagcac
     9541 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
     9601 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
     9661 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
     9721 cacaagttgc cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat
     9781 ttttcgactt cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg
     9841 ctggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
     9901 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggac gatggcaacg
     9961 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
    10021 catctatggg cccattgaca ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag
    10081 tctttgcctg gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
    10141 acttcctggt gccgcttttc ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc
    10201 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca
    10261 caactccttc agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact
    10321 gaaaaaaaat atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg
    10381 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    10441 ccaggttggg tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    10501 gaagtgctgg cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac
    10561 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    10621 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    10681 gattatatcc gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg
    10741 aagtggatcc tcgaccatgt ggaaggctct cgcgagcgtg cacgtcgtgg tgaattgctg
    10801 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    10861 gattacacca acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    10921 aaaatgctgg aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    10981 gaagtatacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    11041 atcgccggtg accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    11101 aagaacacct atggcactgg ctgctttatg ctgatgaaca ctggcgagaa agcggtgaaa
    11161 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg
    11221 ttggaaggtg cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag
    11281 ttgattaacg acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt
    11341 gtgtatgtgg ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    11401 ggggcgattt tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg
    11461 gagtctattg cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc
    11521 cgtctgcacg ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc
    11581 cagtccgata ttctcggcac ccgcgttgag cgcccggaag ttcgcgaagt gactgcattg
    11641 ggtgcggcct atcttgctgg tctggcggtc ggtttctggc agaacctcga cgagctgcaa
    11701 gagaaagcgg tgattgaacg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    11761 cgttacgcag gctggaaaaa agcggtcaaa cgcgcgatgg cgtgggaaga acacgacgag
    11821 taatgtaaat gccggatgaa gcgtttatgc cgcatccggt agtcctgaaa ccgtgcgggg
    11881 gcaaccccgc acacatcaat aatcccaccc ttcccctgtg ctacacttcg cgccattcct
    11941 tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa
    12001 tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc
    12061 gcggcggtaa acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc
    12121 attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact
    12181 ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg
    12241 atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat
    12301 gcgccggata tgtatatgga gaagctgatt gtcgggccgg gagccaaagg caccattgat
    12361 ctgaacctgc cgctggcgga taacctgcgc aatgtagcgg cggcgctcgg caaaccgttg
    12421 agcgaactga cggtaacgat tctagctaaa ccacgccacg atgccgttat cgctgaaatg
    12481 cagcaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc
    12541 acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc
    12601 gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg
    12661 cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca
    12721 cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc
    12781 agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt
    12841 agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc
    12901 attcgccgca ttcagtccat ccactatctg gatcgcaaag acccggaaat gcaggtgcac
    12961 atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt
    13021 tcggagaacg aagataaggc aagttaatca aaacaggaga aaaacatggc tgattgggta
    13081 acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac
    13141 gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc
    13201 gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt
    13261 tacctggtca ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccaggc
    13321 gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccggac
    13381 tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg
    13441 caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat
    13501 gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta cgaagggaaa
    13561 ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cgggcggata
    13621 ccggcgttaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa
    13681 accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg
    13741 aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag
    13801 cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct
    13861 tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtga tgatgaccac
    13921 agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc
    13981 cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct
    14041 tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac
    14101 ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat
    14161 gtctggcgag ctccagagct ttattcacca gtaaggcatc ttcttcattc ccggaaattg
    14221 ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa
    14281 cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc
    14341 atcaagctca acttgcccct ccagatcctt aatcttgtag gccacgctgg ccccttcatc
    14401 tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc
    14461 gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc
    14521 gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct
    14581 ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac
    14641 aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc
    14701 agaaggattt tccgggcatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata
    14761 gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat
    14821 cctgggtatc tttaccatat ttattttcgc cttgtgtgcc aatgaacgct ccaagatcga
    14881 taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat tgccagacac
    14941 ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc
    15001 atgcgccgga acgcccgcga tcatgcaagc gcttaacggt tactgccgct gttggccaaa
    15061 gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg
    15121 agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc
    15181 caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt
    15241 tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc
    15301 agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt
    15361 ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc
    15421 cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact
    15481 ttaacagcca gaatcccagc ctgccactga acgaatttcg tgccatcgac agcagtcccg
    15541 acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag
    15601 cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg
    15661 ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca
    15721 cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctgctaacgg
    15781 cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg
    15841 acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat
    15901 cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac
    15961 tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg
    16021 gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca
    16081 catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc
    16141 ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag
    16201 ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga
    16261 aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg gccactctga
    16321 acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa
    16381 agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg
    16441 caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc
    16501 ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc
    16561 tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt
    16621 tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa
    16681 cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg gtgcttctct
    16741 gaaagctgac gctttcgcag taatcgttaa agctgcagaa gcggctaaac aggcttaagt
    16801 ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca
    16861 aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat
    16921 ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt
    16981 agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc
    17041 gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac
    17101 ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca
    17161 catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg
    17221 agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa
    17281 aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag
    17341 gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt ggaccattaa
    17401 gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt
    17461 gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact
    17521 cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata actatcatca
    17581 ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag
    17641 ggtaacgcaa aatcgtgaca aataaaaaac ccggtcacga gaaaccaccg ggtttgataa
    17701 ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc
    17761 tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt
    17821 ggaaacgcat tttcgtactt tttcgccacc tcagcgtcgc gcggacggta gtagtttttc
    17881 gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct
    17941 ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata
    18001 gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga
    18061 gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg
    18121 gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc
    18181 tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag
    18241 ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta
    18301 atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg
    18361 gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc
    18421 ccgcgttccg caattgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca
    18481 ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgaatcacc
    18541 acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac
    18601 aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg
    18661 gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat
    18721 gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa
    18781 gattggctat aacttgtggg tatatgttga gggattaaaa aggcggaaga aatccgcctc
    18841 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc
    18901 tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc
    18961 atcaggcatt tttgcttctg tcatcggttt cagggtaaag gaatctgcct ttttccgaaa
    19021 tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt
    19081 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg
    19141 accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
    19201 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
    19261 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
    19321 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
    19381 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
    19441 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
    19501 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
    19561 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
    19621 accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
    19681 gccgataacc accagcgcgt cgatcccacg ttttttcagg ttttcgatag ccacggcgcg
    19741 gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt
    19801 gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
    19861 atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc
    19921 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
    19981 aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg aagtcttctt
    20041 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
    20101 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
    20161 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
    20221 atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
    20281 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
    20341 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
    20401 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
    20461 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa
    20521 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
    20581 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
    20641 ttgcgtccgg cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc
    20701 cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag atatcagatg
    20761 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
    20821 cgccgcgagg gactctgctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
    20881 ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac
    20941 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
    21001 tagcaacgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
    21061 ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc
    21121 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc cccacatcat gtgggggaag
    21181 acagggatgg tgtctatggc aaggaaaaca gggtttacta ctgggaacgt gagttgctac
    21241 tactcaatag cttcaacgat gaactttttt gccattgcgt cacgtcacgc aactgctcca
    21301 ttcgttgttg atgtttctcg tttaaaaccg cttgctgctc cggcgttaac aggcgataca
    21361 tttggttgcg gacttttgcc atctcaacct gacgagcaat ttgctcattc gccatttttt
    21421 ctgcctgtgc gcgcacagcg ttttcatcaa aattttctgc ggtgacaagg cgatgcattg
    21481 tctccagttc gctaacatta acaggaggct gttcgtgccg ggcctgttgc ataagatctc
    21541 gcatctgctg acgctgatgt tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac
    21601 tgcgctgcgt aagttcttca cccggatgcc agttatcgcc tgaaccgact tcagcagcgt
    21661 ggcttaatga actgactgcc agcgttgagg ccatgacggc agcggtaact atgcgcatca
    21721 tttgctccca aaatctttct gtcgcgattc aacgatagag agtttacgat tcaggctgca
    21781 aacatgcgtc agggggtgta aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt
    21841 aatttctgcc tcggaggtat ttaaacaatg aataaaatcc tgttagttga tgatgaccga
    21901 gagctgactt ccctattaaa ggagctgctc gagatggaag gcttcaacgt gattgttgcc
    21961 cacgatgggg aacaggcgct tgatcttctg gacgacagca ttgatttact tttgcttgac
    22021 gtaatgatgc cgaagaaaaa tggtatcgac acattaaaag cacttcgcca gacacaccag
    22081 acgcctgtca ttatgttgac ggcgcgcggc agtgaacttg atcgcgttct cggccttgag
    22141 ctgggcgcag atgactatct cccgaaacca tttaatgatc gtgagctggt ggcacgtatt
    22201 cgcgcgatcc tgcgccgttc gcactggagc gagcaacagc aaaacaacga caacggttca
    22261 ccgacactgg aagttgatgc cttagtgctg aatccaggcc gtcaggaagc cagcttcgac
    22321 gggcaaacgc tggagttaac cggcactgag tttaccctgc tctatttgct ggcacagcat
    22381 ctgggtcagg tggtttcccg tgaacattta agccaggaag tgctgggcaa acgcctgacg
    22441 cctttcgacc gcgctatcga tatgcacatt tccaacctgc gtcgtaaact gccggatcgt
    22501 aaagatggtc acccgtggtt taaaaccttg cgtggtcgcg gctatctgat ggtttctgct
    22561 tcatgatagg cagcttaacc gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg
    22621 tgttgatgtt ggttttgatg ttacccaagc tcgattcacg ccagatgacc gagcttctgg
    22681 atagcgaaca gcgtcagggg ctgatgattg agcagcatgt tgaagcggaa ctggcgaacg
    22741 atccgcccaa cgatttaatg tggtggcgac gtctgttccg ggcgattgat aagtgggcac
    22801 cgccaggaca gcgtttgtta ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca
    22861 gcgaaatgca gatcattcgt aactttattg gtcaggccga taacgccgat catccgcaga
    22921 agaaaaagta tggccgcgtg gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata
    22981 attaccaact ttatctgatt cgtccggcca gcagttctca atccgatttc attaacttac
    23041 tgtttgaccg cccgctatta ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt
    23101 tgtggttggc ctggagtctg gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag
    23161 ttgcccaggg aaacttacgc cagcacccgg aacttgaagc ggggccacag gaattccttg
    23221 ccgctggtgc cagttttaac cagatggtca ccgcgctgga gcgcatgatg acctctcagc
    23281 agcgtctgct ttctgatatc tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg
    23341 gtacggcgtt actgcgccgt cgtagtggtg aaagcaagga actggagcgt attgaaaccg
    23401 aagcgcaacg tctggacagc atgatcaacg atctgttggt gatgtcccgt aatcagcaaa
    23461 aaaacgcgct ggttagcgaa accatcaaag ccaaccagtt gtggagtgaa gtgctggata
    23521 acgcggcgtt cgaagccgag cagatgggca agtcgttgac ggttaacttc ccgcctgggc
    23581 cgtggccgct gtacggcaat ccgaacgccc tggaaagtgc gctggaaaat attgttcgta
    23641 atgctctgcg ttattcccat acgaagattg aagtgggctt tgcggtagat aaagacggta
    23701 tcaccattac ggtggacgac gatggtcctg gcgttagccc ggaagatcgc gaacagattt
    23761 tccgtccgtt ctatcggacc gatgaagcac gcgatcgtga atctggcggt acaggattgg
    23821 ggctggcgat tgttgaaacc gccattcagc agcatcgtgg ctgggtgaag gcagaagaca
    23881 gcccgctggg cggtttacgg ctggtgattt ggttgccgct gtataagcgg agttaaactc
    23941 cgcatttgta ggcaggataa ggcgtttacg ccgcatccgg catttgagca ggatgcctga
    24001 tgcgacgctg atagcgtctt atcaggccta cactccgggg gttattttcc ccacaaccgg
    24061 cgggaaaaat cttcgatctt gccgctcagt cggcgctttt gcatcgtccg cgtccagctt
    24121 ttcgataacc cggcggcgga gagtaaacgg tgatattgat cgtcatcaaa cggcatatgc
    24181 catgcgatgg cggcagcctc ctgcacggtc acatcgctga cacgggaaac caactccagc
    24241 ggcgcgtccg cagacacttt ccctggcgct atcacgctat acaaccaccc caccttacct
    24301 gtgttttgca tcaactgcgc tatatcgctg atatcaaaat ggtaattgag tttatagcaa
    24361 ggcgagcgcg gctggctgac ctgaattaat gcctctcccc agcggaaaat atcgcccata
    24421 taaacattac tttccgtcag gccgtcggtt gagaggtttt caccaaacgc aggcgcaaca
    24481 aacaactccg cctgttccgg aaattcccgc gcccagtaga gataatgctc acgaggataa
    24541 tgacacagcg ctctgtctgg cccgccgtga acctttttct ccgcctgctc gtcaccttcc
    24601 agccccagct ccgtcagcat caactcacca tcaacctgga ttttggcaat tgcgctgggt
    24661 ttgccttcgg gataagcctg aatcttgcct gtgtatacat caaccggata tcgcatctgt
    24721 tccctctcca cgagaaattt ccccattaga ccatatttat tttatgtcag gcgtgctgtt
    24781 ttcgcggtaa gtatccaggc aggaactcaa tagcaaaccg gacgaacgtt agttcatccg
    24841 gctgtgggga ttaaggaagc aggtttattt caccgtacct aaaatttcga ttttcgctgc
    24901 tctggctttg actttacgtg accagataga ggtaagaatt ggcaccagaa tcgaagtcac
    24961 aatgaccgcc gtcgctacca gcgaagttgc tgccggagcc atcggtttaa acgcaggcac
    25021 catttctgca atcagcacag gtgtcgctac cgcggcccct gcggaactgg aagcggcaat
    25081 tccggcagtg ccatcgccac cgccaatcaa tttatcggcg ataatcaacg gaataccggt
    25141 cacgataatt actgccacac ccaacaggat ccccagcaaa ccagtctggg caatcacagt
    25201 caaatcaatg gtattgccca gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt
    25261 gctgaaaaat tctcgtaatt cagggtcaag gttccccagg gcaaagccca ccaggaacgg
    25321 taatactgcg ccgacgaaaa catgcggttc aaacgaggca atccctgcag tgcccagaat
    25381 aatcatcgtc atgagcggcc cggactccaa cgacatcaac acaaatgccc cagcttcttc
    25441 ttttgtgccg tactgctgca tgatggaagc gtaaagtccg ccattggtca tatccatcgc
    25501 cgccaccagc gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg
    25561 aataatgcgc gaggcaattg ccgcaaccac ccacgcgacg gcaatttttg ttaccaccag
    25621 cgtaccggat ttacgcaata ccgttcccgt cgcgcttaat tttattgacg cccccatgca
    25681 aaaaaaccac accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc
    25741 aaaatatttc cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac
    25801 gagcatcatc ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg
    25861 tcggcatatt tttaagggct gatgcgtcat tcctcaatgg agcatcagtg ttgatggaaa
    25921 cgaagataga cattcattta atcaataaaa gtgatcaaaa ccacatcaat tgaaacactg
    25981 ttttattttt atcggatcat tgtttttatt ttgaaattga tcacaaaaaa accaccgccg
    26041 ttggcgatgg ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg
    26101 caaatgatta ttttttcgcc gcgaaacgcg ccgcagcttc gtcccagttc accacgttcc
    26161 agaactcttt gatgtagtcc gggcggcggt tctggaattt caggtagtaa gcatgttccc
    26221 acacatccag gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg
    26281 gagaatcctg gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg
    26341 cccagccgga accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa
    26401 cggagccgaa gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt
    26461 tcagaccttt ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg
    26521 ttttcttgtc tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg
    26581 caaattctgg caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt
    26641 tggtgtggtg gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat
    26701 acggcaggga tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg
    26761 ttaatgccgc gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat
    26821 caatgccgta cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa
    26881 atcacccact taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt
    26941 taatcgacag cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat
    27001 gaggataaga agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg
    27061 gccagtgcag cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc
    27121 atgtggtcag tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg
    27181 ttactaccgg atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt
    27241 tttctgttcg gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat
    27301 ctcggcctgt cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg
    27361 atgacgccaa ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg
    27421 acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga ttgtgactcg cgccgggcag
    27481 ttgaaagagc gcaaaatggg cattaaagcc gaagagttca acctgaaaaa agggctggtg
    27541 ctggcggtga tgtgcggcat tttctctgcc ggtatgtctt ttgcgatgaa cgccgcaaaa
    27601 ccgatgcatg aagccgctgc cgcactgggc gtcgatccac tgtatgtcgc tctgccaagc
    27661 tatgtcatca tcatgggcgg cggcgcgatc atcaacctcg gtttctgctt cattcgtctg
    27721 gcaaaagtga aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt
    27781 cacaatgtgt tactctcagc actgggcggg ttgatgtggt atctgcaatt ctttttctat
    27841 gcctggggcc acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg
    27901 agtttctatg tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaatgca
    27961 ggtcgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgccaac
    28021 atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg
    28081 ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca
    28141 cattcggtcg aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga
    28201 cacactctga cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc
    28261 tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga
    28321 cttttcaggc tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt
    28381 ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac
    28441 agcaactcag ccatttcgtt agcaaacgac acatgctgac tactttcatg ctcaagctga
    28501 ccgataacct gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct
    28561 gcgctggcgc taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc
    28621 gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg
    28681 tcagcatgaa tgtaaaagag atcgccacgg gtaatgcgat aagggcgatc gttgagtaca
    28741 tgcagaccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca
    28801 aaaacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa
    28861 tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt
    28921 tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaaacc
    28981 atcccgtccc tgacgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga
    29041 aaagtggtta ctgtcgctga atccacagcg ataggcgata tcagtaacgc tggcctcgct
    29101 gtggcgtagt agatgtcggg ctttcatcag tcgcaggcgg ttcaggtaac gctgaggcgt
    29161 cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc agcgaaagag aaaattgctc
    29221 cgccacggca tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa
    29281 gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag
    29341 cagtaattgc ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc
    29401 ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg
    29461 ccagtgggaa ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag
    29521 aaactgaaat cgatccggcg agcgatacag cacattagtc agacacagat tatcggtatg
    29581 ttcatacaga tgccgatcat ggtcgcgcac aaaacagacc gtgccaccgg tgatggtata
    29641 gggctgccca ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa
    29701 atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc
    29761 gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg
    29821 tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa
    29881 ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc
    29941 atcacatcac cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc
    30001 caatggccca ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg
    30061 gtcgtaatga aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc
    30121 tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga
    30181 cgctgcgcga aatccatcgt tttaacaatg ggctgcatag tcagaacggc tatgtcacct
    30241 gggatgtgga tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag
    30301 ggattcgtat cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc
    30361 aacagggtca gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga
    30421 tggcgcaggc acaacaacaa ctcggcaaac gcgatattta tcaacgtagc ggcatccagt
    30481 ttctgccctt caatacgctt tatcagttac gggcgctgac ggagcaacaa cctgaactta
    30541 ttccacacat tgctcacgct ctgctaatgc cggattattt tagttatcgc cttaccggca
    30601 agatgaactg ggagtacacc aacgccacaa ccacgcaact ggtcaatatc aatagcgacg
    30661 actgggacga gtcgctactg gcgtggagcg gtgccaacaa agcctggttt ggtcgcccga
    30721 cgcatccggg taatgtcata ggtcactgga tttgcccgca gggtaatgag attccggtgg
    30781 tcgccgttgc cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac
    30841 gcgccgctta tctctcttct ggtacctggt cattgatggg cttcgaaagc cagacgccat
    30901 ttaccaatga cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct
    30961 atcgggtgct gaaaaatatt atgggcttat ggctgcttca gcgggtgctt caggaacggc
    31021 aaatcaacga tctcccggcg cttatcgccg cgacacaggc acttccggcc tgtcgcttca
    31081 tcatcaatcc caatgacgat cgctttatta atcctgaagc gatgtgcagc gaaattcagg
    31141 ctgcgtgtcg ggaaacggcg caaccgatcc cggaaagtga tgctgaactg gcgcgctgta
    31201 ttttcgacag tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg
    31261 gtgaagattt ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc
    31321 agctatgcgc cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc
    31381 tcggcaatat cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc
    31441 gtcaggtcgt cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa
    31501 ttgcccacta tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac
    31561 cactcaactg gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga
    31621 tgtcgaggag gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg
    31681 cgatgatgtt tccggttttg aaaacccgga aggttcgctg accggtggga ttcaagctac
    31741 tggtaattat ccgggcaaag cgcgtaatgc cagtgagcta cgtaccgatc tggaacaggc
    31801 tatgcggctg attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga
    31861 tacgccagtc tcgcgcgatc agatcaaacc agagcacttc aaaaactggg ttgaatgggc
    31921 gaaagccaat cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc
    31981 cgatggcttt acgctttccc atgccgacga cagcattcgc cagttctgga ttgatcactg
    32041 caaagccagc cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat
    32101 gaacatctgg atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca
    32161 gcgtctgctg gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat
    32221 cgacgccgtt gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa
    32281 tgagttttac atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca
    32341 cttccacccg actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtgccgca
    32401 gttgctgctg cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga
    32461 tgatgaaacc caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca
    32521 tatcggcctt gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac
    32581 acgcaatatg aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa
    32641 gctggaagcg gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt
    32701 gccgtggcag gcggtctggg aaatgtattg ccaacgtcac gatacgccag caggtagcga
    32761 atggctggag agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact
    32821 gccggatgcg gcgcgagcgc cttatccggc ctacaggtcg aatgtccatg cgggctgtca
    32881 ccctcacccc ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa
    32941 cggagctgaa cactgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgaa
    33001 gggaacaagc agcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc
    33061 aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc
    33121 tgaaaggctg ggacgagcgc aacggcggta acctgacgct gcgcctggat gacgccgata
    33181 tcgcgcctta taaagacaac ttccatgcac aaccgcgcta tatcccgctc agccaaccca
    33241 tgcctttact ggcaaataca ccgtttattg tcaccggctc cggcaaattc ttccgtaacg
    33301 tccagcttga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct
    33361 accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact
    33421 ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact
    33481 gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta
    33541 ctcgccaact gtgggaaggc agcaccgagt gtctggtagt gttcccggat ggcgttggca
    33601 ttttgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc
    33661 aaaaacattc gctggtgttg tggcccttcc acggcgtctt cggcagcggg tcgacgttgg
    33721 atgaaacatt cggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt
    33781 attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc
    33841 gcttcggcgt tacaccacta gctagtgcgc tggcgctgta aggagcaaac atgatccgca
    33901 aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt cggcataacc
    33961 ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac tacgccattt
    34021 acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct gaagaacggt
    34081 ggaatgcggt agccagcact gatgtttgcc aacgttggtg gaaatatatg accgatgtta
    34141 tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc
    34201 cgtaattgcc caatgtgcca gagatgcctc cggcacaata ttttaaattt cgatttgaaa
    34261 aattaaacat attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca
    34321 caaaaataat gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc
    34381 attactccgc aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc
    34441 gcctctcatc acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc
    34501 gattaatggc agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt
    34561 attccgttct gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac
    34621 accagttcct gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg
    34681 gtgtcgccgt gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc
    34741 gcaaagcgca ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct
    34801 gcctcggcgt gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc
    34861 gcaaaattat tcacaaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc
    34921 ttgccttgtt aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt
    34981 attgtcgcga tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc
    35041 ctggaacaga aagcccgttc tctcggtcat accattaaag ttgaaactca aggatcaagc
    35101 ggcgtcgaaa accgcttatc cagcgaagag attgccgctg ccgattacgt cattctcgct
    35161 accgggcgtg gcctgagcgg tgacgatcgc gcgcggtttg ccgggaagaa ggtttatgag
    35221 attgccatct cccaggcgct gaaaaatatc gatcagattt tcagtgaatt accaacaaac
    35281 tcgcatcttt ttgctgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc
    35341 agcgtgatga gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggc
    35401 ggtattctgg tggcgcttgc caacatgctg gtgcagttcg gtttgccata taccgatatg
    35461 tcgaaaggtg cgccgtcgtt tacctgggta gttgaatcca tcggttacct cggcttcacc
    35521 tttatgatcc ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc
    35581 gctccggcgt ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg
    35641 ggtgcgggat tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg
    35701 ttccgcaaag tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg
    35761 ttcgttactc tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc
    35821 gacctcatgg gcgggctgct ccacttcctg aacaccattc ctccatcaat gaagtttgcg
    35881 gcagcgtttc tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc
    35941 gcctggttct tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc
    36001 ggcgttgtcg cgttgatgcc gcccgtcgcg gcgggtcttg cgacgttcat tgcgcccaaa
    36061 ctgtttaccc gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc
    36121 gcaaccgaac cggctattcc ttacgctctg gcagctccgt tgccgatgat taccgccaat
    36181 acgctggcgg gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggcg
    36241 cctggtttgg gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat
    36301 ctggtgctgg cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg
    36361 tggttacgcc gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact
    36421 gcaacagttg tgcgaagcca gcgccgtcag cggcgatgaa caggaagttc gcgacatcct
    36481 gataaacacg ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt
    36541 tgcccgtaag gggaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg
    36601 ctttatggtc acccacatcg acgagagcgg ttttctgcgt tttaccacca ttggcggctg
    36661 gtggaatcag tcgatgctca accaccgggt aaccatacgc acacacaagg gagtgaaaat
    36721 ccctggtgtg attggttccg tcgcgcctca tgcgttaacg gaaaagcaaa agcaacaacc
    36781 gctgtcattt gatgagatgt tcattgatat tggcgcgaac agtcgcgatg aggtggaaaa
    36841 acgcggtgtg gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga
    36901 agataaagtg gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact
    36961 attgcagacg gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga
    37021 agttgggcta cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt
    37081 gctggatacc gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa
    37141 actgggccag gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact
    37201 ggtagcagcg ttaaaaagct gtgccgcaca taacgattta ccgctgcaat tttccaccat
    37261 gaaaaccggt gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt
    37321 tgcgctgtgc ctgccaaccc gctatctgca cgctaacagc ggtatgattt caaaagccga
    37381 ttacgatgct ctgctcacgt tgatacggga ttttctgacc accttaaccg cggagaaagt
    37441 caacgcgttt agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt
    37501 gtcgatctgc tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt
    37561 tcaggcagga ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc
    37621 cgcatttccg ccagtggtag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt
    37681 tttcagttgc tgcaaaagca cgataacgac gatcggctgc tggcactgtt attactgaat
    37741 acctttactc cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtcgca
    37801 gagcgtctgc cccggttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct
    37861 ggtttgggcc atttcattga tgagacagaa gagaaacgcg ttatcttgct ggcgaacttg
    37921 ctgcgcaaag atccgttttt aattccgctg gcgggcgtaa cacgagacaa ccttcagcat
    37981 ttatccacgg cctgcgacaa ccaacaccgc tggccgctca tgcagggtga ttatctctcc
    38041 agcctgattc tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaatat
    38101 cccggtgacg agataaaaca aatcgttgaa catagcggtc tgtttcttgg tgataacgct
    38161 gtaagaaccc tgacaggttt gatagagaaa cagcatcagc aagcgcagat aatttctgcc
    38221 gataatgtgc agcggttgct gcaaagggtg ccgggcatcg cgtcattgaa tattattgat
    38281 acgcagctgg ttgagaacat taccgggcat ttattacgtt gccttgccgc accggtgtgg
    38341 attgctgagc accgccaaag cagcatgaat aacctgaaag ccgcctggcc tgcggccttt
    38401 gatatgagtc tgcactttat tacgctactg cgtgaacagc tcgatattcc ccttttcgac
    38461 agcgatctga tcggtttgta ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag
    38521 ccgatcattt tgctctcgga ccagaacgcg attgccacta ttaatcagct cgccattgag
    38581 cgcgatgttt tacattgtcg ggtgattatt gcccgtagct taagcgaact tgttgccatt
    38641 cgcgaagaga ttgagccgtt attgatcatt aacaacagcc attatttact ggatgacgcg
    38701 gtaaataatt acatcaccgt aaaaaatatc attacggctg ccggtatcga acaaataaaa
    38761 cattttctgg cgacggcatt tattcgccaa cagccggagc gttttttctc tgcccccgga
    38821 agttttcatt attcgaatgt acgcggtgaa agctggcaac atattacccg gcaaatttgt
    38881 gcgcaattag tggcacaaca ccatattacc gccgatgaag cacaacgcat catcgcccgc
    38941 gaaggcgaag gtgaaaacct gattgttaat cgcctcgcca tcccacattg ctggagcgaa
    39001 caggagcgac gttttcgtgg attttttatt accctcgccc aaccagttga ggtgaataac
    39061 gaagtcatta accatgtctt gatcgcctgc gccgccgccg atgcgcgtca tgagctaaaa
    39121 atatttagct atctggcaag cgtattgtgt cagcatccgg cagaggttat tgccgggtta
    39181 acaggatatg aggcatttat ggagttactt cacaaggggt gaactgtgtc ggatgcgccg
    39241 catccgacac tgcatttatc atcaaaaacg tttaacgcag gctattatag tcatcaacca
    39301 tttcgttgta ctcgcgtaat gctctcggga aagagtcttc gaccatccag cttgagtttg
    39361 cgtcctgaag ttgctcttta tcccagtcag agtacgggac tttatctctg atgcggcgaa
    39421 cgtatttttt agcctcgagt tgatactggc ctgccgaatt aataaacgag aaattcatgc
    39481 cgcctttatc cgcttctttg gcctgcgcca ctagcgtttc cagttcagac actttcttca
    39541 ttgccgcatc aacatcaaac ttctcttgcg atatcaggtt attaatttgt ttcgctgaaa
    39601 tcatgactgc cagagagtag tagtggaatg ttttcccttc tctttcttca atatttttca
    39661 gttccgcaag ctggcgctta tcgttaattt cctgaatcgc cgcatgataa gattctgcta
    39721 caggttcaaa ggcttccaga ttttttaaga atgtctggtg cagcgttttc cctttcgcaa
    39781 acgcatcgtc tttataattt tcctgggtgt aatatttatc catttcgtta atggtattac
    39841 ccaacgccac ggcagcatcg atataactca ccgccacgcc atcaattggt tgcagcgcag
    39901 gcgttaacgc cacagcactt tttacgcctt tttcacactc agcgaggttg gattcactaa
    39961 tgccgtagat gccataaaca gtacgctctt caccggtagg cccctgttta aaatctttca
    40021 gccagtcagc ataacgcgcc agactgctct gtacagggat ctgtaacttg ttatagcact
    40081 tgatatagac atccattttt tcgtcgaaaa ccgcttgttc atcgaccacc ggttgcgcag
    40141 gtgtttcagg tttagcttct gcttttgcaa cgggagcttc aggagctggt gcggcaggtt
    40201 gactattggc aggcgggggc gtttctgttt cggctttttt atcatcacaa cccgtcagac
    40261 cgagggccat gatggcgact ataatagcgg aagataa
//
LOCUS       DAZFWP020000023        39875 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFWP020000023 DAZFWP020000000
VERSION     DAZFWP020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.023"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHK0Z_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHK0Z_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU4501175.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHK0Z_RS05570"
                     /old_locus_tag="pgaptmp_001114"
     CDS             complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHK0Z_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU4501176.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVESILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHK0Z_RS05575"
                     /old_locus_tag="pgaptmp_001115"
     CDS             complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHK0Z_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU4501177.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHK0Z_RS05580"
                     /old_locus_tag="pgaptmp_001116"
     CDS             complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHK0Z_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU4501178.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5253..5372)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHK0Z_RS05585"
                     /old_locus_tag="pgaptmp_001117"
     CDS             complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHK0Z_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU4501179.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHK0Z_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHK0Z_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4501180.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHK0Z_RS05595"
                     /old_locus_tag="pgaptmp_001119"
     CDS             complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHK0Z_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU4501181.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6909..7625
                     /gene="arcA"
                     /locus_tag="ACHK0Z_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             6909..7625
                     /gene="arcA"
                     /locus_tag="ACHK0Z_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU4501182.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7685..9037)
                     /gene="creD"
                     /locus_tag="ACHK0Z_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     CDS             complement(7685..9037)
                     /gene="creD"
                     /locus_tag="ACHK0Z_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU4501183.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9095..10519)
                     /gene="creC"
                     /locus_tag="ACHK0Z_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     CDS             complement(9095..10519)
                     /gene="creC"
                     /locus_tag="ACHK0Z_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU4501184.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10519..11208)
                     /gene="creB"
                     /locus_tag="ACHK0Z_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     CDS             complement(10519..11208)
                     /gene="creB"
                     /locus_tag="ACHK0Z_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU4501185.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11221..11694)
                     /gene="creA"
                     /locus_tag="ACHK0Z_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     CDS             complement(11221..11694)
                     /gene="creA"
                     /locus_tag="ACHK0Z_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU4501186.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11905..12774
                     /gene="robA"
                     /locus_tag="ACHK0Z_RS05625"
                     /old_locus_tag="pgaptmp_001125"
     CDS             11905..12774
                     /gene="robA"
                     /locus_tag="ACHK0Z_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU4501187.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12771..13418)
                     /gene="gpmB"
                     /locus_tag="ACHK0Z_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             complement(12771..13418)
                     /gene="gpmB"
                     /locus_tag="ACHK0Z_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU4501188.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13470..13991
                     /gene="yjjX"
                     /locus_tag="ACHK0Z_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             13470..13991
                     /gene="yjjX"
                     /locus_tag="ACHK0Z_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU4501189.1"
                     /translation="MHQVVCATTNPAKIQSILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14076..14402)
                     /gene="trpR"
                     /locus_tag="ACHK0Z_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             complement(14076..14402)
                     /gene="trpR"
                     /locus_tag="ACHK0Z_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU4501190.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14492..16429)
                     /gene="sltY"
                     /locus_tag="ACHK0Z_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             complement(14492..16429)
                     /gene="sltY"
                     /locus_tag="ACHK0Z_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU4501191.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16640..18307
                     /gene="ettA"
                     /locus_tag="ACHK0Z_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             16640..18307
                     /gene="ettA"
                     /locus_tag="ACHK0Z_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU4501192.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18614..19846)
                     /gene="nadR"
                     /locus_tag="ACHK0Z_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             complement(18614..19846)
                     /gene="nadR"
                     /locus_tag="ACHK0Z_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU4501193.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19867..21249)
                     /gene="radA"
                     /locus_tag="ACHK0Z_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             complement(19867..21249)
                     /gene="radA"
                     /locus_tag="ACHK0Z_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU4501194.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21298..22266)
                     /gene="serB"
                     /locus_tag="ACHK0Z_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             complement(21298..22266)
                     /gene="serB"
                     /locus_tag="ACHK0Z_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU4501195.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLHS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22372..23016
                     /gene="ytjB"
                     /locus_tag="ACHK0Z_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             22372..23016
                     /gene="ytjB"
                     /locus_tag="ACHK0Z_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGU4501196.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23044..24060
                     /gene="lplA"
                     /locus_tag="ACHK0Z_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             23044..24060
                     /gene="lplA"
                     /locus_tag="ACHK0Z_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU4501197.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            24092..24355
                     /locus_tag="ACHK0Z_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /pseudo
     CDS             24092..24355
                     /locus_tag="ACHK0Z_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(24516..25235)
                     /gene="deoD"
                     /locus_tag="ACHK0Z_RS05685"
                     /old_locus_tag="pgaptmp_001137"
     CDS             complement(24516..25235)
                     /gene="deoD"
                     /locus_tag="ACHK0Z_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU4501198.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25315..26538)
                     /gene="deoB"
                     /locus_tag="ACHK0Z_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             complement(25315..26538)
                     /gene="deoB"
                     /locus_tag="ACHK0Z_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4501199.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26590..27912)
                     /gene="deoA"
                     /locus_tag="ACHK0Z_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             complement(26590..27912)
                     /gene="deoA"
                     /locus_tag="ACHK0Z_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU4501200.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28039..28818)
                     /gene="deoC"
                     /locus_tag="ACHK0Z_RS05700"
                     /old_locus_tag="pgaptmp_001140"
     CDS             complement(28039..28818)
                     /gene="deoC"
                     /locus_tag="ACHK0Z_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU4501201.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29076..30626
                     /gene="yjjI"
                     /locus_tag="ACHK0Z_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             29076..30626
                     /gene="yjjI"
                     /locus_tag="ACHK0Z_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGU4501202.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30598..31461
                     /gene="yjjW"
                     /locus_tag="ACHK0Z_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             30598..31461
                     /gene="yjjW"
                     /locus_tag="ACHK0Z_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU4501203.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(31876..32658)
                     /gene="yjjV"
                     /locus_tag="ACHK0Z_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             complement(31876..32658)
                     /gene="yjjV"
                     /locus_tag="ACHK0Z_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4501204.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(32655..33728)
                     /gene="yjjU"
                     /locus_tag="ACHK0Z_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             complement(32655..33728)
                     /gene="yjjU"
                     /locus_tag="ACHK0Z_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU4501205.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(33850..34011)
                     /gene="ytjA"
                     /locus_tag="ACHK0Z_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             complement(33850..34011)
                     /gene="ytjA"
                     /locus_tag="ACHK0Z_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU4501206.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(34138..34743)
                     /gene="osmY"
                     /locus_tag="ACHK0Z_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             complement(34138..34743)
                     /gene="osmY"
                     /locus_tag="ACHK0Z_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU4501207.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(35136..36725)
                     /gene="prfC"
                     /locus_tag="ACHK0Z_RS05735"
                     /old_locus_tag="pgaptmp_001147"
     CDS             complement(35136..36725)
                     /gene="prfC"
                     /locus_tag="ACHK0Z_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU4501208.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(36816..37493)
                     /gene="yjjG"
                     /locus_tag="ACHK0Z_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             complement(36816..37493)
                     /gene="yjjG"
                     /locus_tag="ACHK0Z_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU4501209.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(37508..37954)
                     /gene="rimI"
                     /locus_tag="ACHK0Z_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             complement(37508..37954)
                     /gene="rimI"
                     /locus_tag="ACHK0Z_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4501210.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(37923..38336)
                     /gene="holD"
                     /locus_tag="ACHK0Z_RS05750"
                     /old_locus_tag="pgaptmp_001150"
     CDS             complement(37923..38336)
                     /gene="holD"
                     /locus_tag="ACHK0Z_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU4501211.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            38439..39470
                     /gene="rsmC"
                     /locus_tag="ACHK0Z_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             38439..39470
                     /gene="rsmC"
                     /locus_tag="ACHK0Z_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU4501212.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            39738..39824
                     /locus_tag="ACHK0Z_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     tRNA            39738..39824
                     /locus_tag="ACHK0Z_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39772..39774,aa:Leu,seq:cag)
BASE COUNT         9417 a        10935 c        10130 g         9393 t
ORIGIN      
        1 ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc ctggaccatg
       61 accgccgtga tgatcatgat gagctttctt atggtggcgc ggcggtggcg gcggtccgtg
      121 tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt ggtcgcgcca
      181 gtgaccgcca tcccagtaat aaccgcgatt atcacgatcg cctatctgta attttactga
      241 cgggactaac gtaatttccg cagcctgcgc tgccatggga gcgaccagaa ccagggaaag
      301 tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt gcccgttatg
      361 ggtcgatatc cggagattac gcgatgagaa cggcggttgt tattctccgc aatcctaatc
      421 tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgttttt ctccataatt
      481 tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga taatgaatag
      541 attttactga tgattcatca tcaatttacg caacgcagca aaatcggcgg gcagattatg
      601 tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca acgtttcacc
      661 gagaatcgat tccacgctct ctttaaattt cgccggatgc gcggtgccga ggaacaagcc
      721 atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag cgtgcggctc
      781 cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt catcatccac
      841 ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact cttccacacg
      901 cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt
      961 gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc
     1021 aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg tcaaatcgcc
     1081 gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcttcctgtg gcagctgcgc
     1141 aacagcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc
     1201 tgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct tcaccagcgc
     1261 ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc ccaacgtaca
     1321 gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa ccactttcac
     1381 attcggtaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg aggtcgcggt
     1441 cagaatggtc actggcttat cgcccgcaat atgggtcagc atttgtgcca taaagcgacc
     1501 gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac cgacatcgct
     1561 ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat
     1621 ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc gggtgacaaa
     1681 atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt catgcggaaa
     1741 aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc tgacctgctc
     1801 gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg cgcccgccgt
     1861 atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct tacccaacca
     1921 gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg tcgggccgga
     1981 gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa
     2041 gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca gcttcgcggc
     2101 aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc gcccgtgcgc
     2161 aatgcaatcc tggcggcgat actgcgccgg taaaatagcc ctggcttctg ccgtcgagac
     2221 tttaatcccc ggatacgcca gcacccacag ccactcatca aaccctggca cttgctggct
     2281 gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg gtgccacgtt
     2341 gtcgtaatga atgctgccgg agattcgtcc ttccagctcg cccatcaaag ccagcaaacg
     2401 agtgtcatta agcggcttgc cacagtgttc attcatcgcc atcagcgccg cgaccaccga
     2461 acaggcgctg gagcctaagc ccgaaccgat tggcatattc ttttccagag tcatcgccac
     2521 tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa cgatattttc
     2581 ccgtggttct gacggcagct tatcggcaaa gcgtccgagg ttgttgagac tgaatgtctc
     2641 tgccgcctca accgtgacta catctccgag caatgcacca tcaacaggtg tcaccgccgc
     2701 cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat aaactttaac
     2761 catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag acaccggcag
     2821 ctgtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc tgataatagt
     2881 ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga tcattaccat
     2941 ccacttcggc aatcttcacg cggcaggcac catcttcatc aatattgcca acatagcgca
     3001 aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg tcgagctgtg
     3061 acagattcgc cataaaagcg gcaacatcac cctcagcgtt aaactctgcg ggcagcacag
     3121 gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgagcg agaatcaata
     3181 gcttacgcgc tacatccata ccagaaagat catctcgcgg atccggttcg gtataaccca
     3241 tttcccgcgc cagagtagtc gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga
     3301 tataagaaag cgaacctgaa agaatgccgg agaacttcat caattcatca ccagcattga
     3361 gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg tcatagagga
     3421 atttacgccg cgatttttcc gccgcatgac gcaacagatg gtagtaatcc atcgacgagg
     3481 tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaagaag tcggcatatt
     3541 gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc agatgatatt
     3601 ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgc gccagttctt
     3661 cctgccagtt ttccagattt aggccatgca cattggtgag cagtgccttc gagttggcaa
     3721 caccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgttga cgcttcagtt
     3781 gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg ataacctgat
     3841 cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca tcgttattta
     3901 ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg ttgatattgg
     3961 cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc ataccatcac
     4021 ctaccaccga gataatggcc agccgttccg tcaccgccag cggctccagt aagccttctt
     4081 tcagttccag gtagaactct tcctgcattg cccgttcagc tcgcacacag tcgctttgtg
     4141 gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc acggaaatac
     4201 gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc gaccattcct ttcatccccg
     4261 ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg accggtaatt
     4321 cgtcttcatc acgactggca ccaatgagcg tacctggtgc ttgaggattt ccggtatttt
     4381 taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg tgaagaactt
     4441 tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc aacaacctcg
     4501 catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc
     4561 aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc
     4621 caagtaccac cagttcgcct ttttcattac cggcggtgaa acctgccatc aacaccatgt
     4681 gatcagccgg aatgcggctt gccgcaatac ggcgggtgga ctcagcaata tcgacggtag
     4741 attcgaggta atgccccact gccagcagtt tttcgaccgg atcgataacg gtaacgttgt
     4801 gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg ccacggcaaa
     4861 tcagcgcagc gttgatgcta tccgggcact gccccaacaa actaatgcca tgcaggacat
     4921 gttttatttg ggcaaattcc tgatcgacga aagttttcaa ttgcgccagc gggaaccccg
     4981 gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat
     5041 tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg tggttggtga
     5101 ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg ctttccagaa
     5161 tatcggcaac acgcagaaaa cgttctgcat ttgccactga tgtaccgccg aacttcaaca
     5221 ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact gtcaggtgcg
     5281 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
     5341 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
     5401 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
     5461 taatttactc acggcaggta accagttcag aagctgctat cagacactct ttttttaatc
     5521 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
     5581 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
     5641 gtctcgttgc tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt ttatgtcatc
     5701 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
     5761 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
     5821 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
     5881 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
     5941 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
     6001 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
     6061 gacatcgtgt aacgattcaa ccagtgtcgg gaaaacttta atattatcaa taatatcacc
     6121 agatccatgt gcgacccagc gggtggctgg ctccagatgt gcctgactat cgacaatccg
     6181 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
     6241 ggcgggtgcg accagaataa tcgttatacg catattgcca ctcttcttga ccaaataacc
     6301 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
     6361 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
     6421 aacggttgaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
     6481 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
     6541 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
     6601 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
     6661 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
     6721 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
     6781 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
     6841 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
     6901 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
     6961 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
     7021 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
     7081 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
     7141 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
     7201 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
     7261 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
     7321 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
     7381 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
     7441 aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
     7501 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     7561 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
     7621 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
     7681 aaccttattt ccagatacgt aattgatcgt ccattgtaac ttctttactg gctttcattt
     7741 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
     7801 ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg agcagtcccc
     7861 acatcacacc atccagcaac aacagcgcga gggtaaacaa catgctgttg cgccagcctt
     7921 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
     7981 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacattacca
     8041 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
     8101 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
     8161 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt
     8221 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
     8281 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
     8341 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
     8401 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
     8461 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
     8521 cggtaccggg ttcaatggtc agtgccgttc cgttaacttc aggcgctttc accacaccaa
     8581 taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg atatttggcg
     8641 cattgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
     8701 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
     8761 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
     8821 cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca acgagttttt
     8881 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
     8941 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
     9001 gggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt gcaatggcag
     9061 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
     9121 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
     9181 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
     9241 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
     9301 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
     9361 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
     9421 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
     9481 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
     9541 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
     9601 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
     9661 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
     9721 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
     9781 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
     9841 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
     9901 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
     9961 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
    10021 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    10081 ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac tgaccacgca
    10141 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    10201 cgaacaatac tttaccctgc gaatcggtca tatagacatg atattcattg cgcactttgt
    10261 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca
    10321 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    10381 ttgcggtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    10441 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    10501 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    10561 aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
    10621 atcaacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca tcagttgctg
    10681 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    10741 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    10801 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    10861 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    10921 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    10981 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccactga tatcaggcaa
    11041 accaacgtca agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
    11101 acctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    11161 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
    11221 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    11281 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    11341 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    11401 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatggc
    11461 cgcatcggag gtatcttctg ccaggcccaa tcccccttta ataccaccgg ttttcgcccg
    11521 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    11581 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    11641 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    11701 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    11761 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    11821 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    11881 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    11941 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    12001 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    12061 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    12121 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    12181 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    12241 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    12301 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    12361 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    12421 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    12481 gaacaagagg tattctatac cactgcgtta gcccaggatc aggcagatgg ctatgtactg
    12541 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    12601 ggaaccggcg tgcaggagtt tatcctgaca gtatacggaa cgtgcatgcc aatgctcaat
    12661 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga tgccaaagcg
    12721 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc cgatccgtcg ttaacgctgc
    12781 aactcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacaacccag
    12841 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    12901 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    12961 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    13021 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    13081 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    13141 atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    13201 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
    13261 ctgatgatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    13321 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    13381 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    13441 taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc
    13501 ccgctaaaat tcagtccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    13561 atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc agtgaggaaa
    13621 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    13681 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    13741 aaaacgccag ccagcgcggc gaggcgcgtt cggctacttt gccgttgcct gcggtgattc
    13801 tggaaaaagt gcgtgaaggt gaggcacttg gtcccgtgat gtcgcgttat accggtattg
    13861 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    13921 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcataat gcggtgtatc
    13981 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    14041 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    14101 tccagccact ggcgcagctc aacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    14161 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct ggctcatttc gccgcgcaac
    14221 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    14281 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    14341 cgtaaccact cttggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    14401 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    14461 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    14521 tcatcaacgt cggtttatcc cccatgaaat agcggtagta agcgtcataa gccagcacgt
    14581 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    14641 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    14701 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    14761 caatgttgat gttcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    14821 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    14881 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    14941 agctttgcgg gatctcctta ccgctggtgt agcgtttgaa aagatcgttg taagccagcg
    15001 ggaatcgctc ttccagatga tcccacagct tcccggcgat cgttgcctga acgctaagat
    15061 cccaccattg gttgttgaaa gcataccgcg ccagttgagc ctgctctgtt tttgacttgc
    15121 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    15181 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    15241 tatcaatctt cagctcatac tcttcgccga tgcgttgtgc agcaaccatc gggtagaaac
    15301 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    15361 gcaataaatc cgcctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    15421 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagcgccatt cgcacacggc
    15481 gttcaatcaa cgacgtggat tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct
    15541 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    15601 gatcttcatt aagctgctgc gcctgggcaa gcgacgggat catcagccgt gcattttcag
    15661 catcctgccg cgccacactg gcaaacgcca ccgccgccat ttgacgggta aaatcggtcg
    15721 cgccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    15781 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    15841 tgttacccgc tttcatcgcc aggcggatac gctctaaata cgccagcgga tcttgtttac
    15901 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    15961 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    16021 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    16081 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    16141 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    16201 ggtcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    16261 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    16321 aacgactacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    16381 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    16441 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    16501 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    16561 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    16621 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    16681 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    16741 aattggtgtc ctgggtctga acggcgcggg taagtccacc ctgctgcgca ttatggcggg
    16801 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttatct
    16861 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtctattg aagaagcggt
    16921 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    16981 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga ttattcaggc
    17041 tcacgacggt cataatctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    17101 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tggcgttgtg
    17161 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    17221 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgaaggca ccgtggtggc
    17281 gattactcac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aactggaccg
    17341 cggtgaaggt attccgtggg aaggtaacta ctcctcctgg ctggagcaga aagatcagcg
    17401 tctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    17461 gtgggtacgt cagggaacta aaggccgtca gtcgaaaggt aaagcacgtc tggcacgctt
    17521 tgaagagctg aacagcaccg aataccagaa acgtaacgaa accaacgaac tgtttattcc
    17581 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    17641 cgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcga tcgtcgggat
    17701 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctccg gtcaggaaca
    17761 gccggacagc ggcaccatca ctttgggtga aacggtgaaa ctggcatcgg ttgatcagtt
    17821 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    17881 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    17941 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    18001 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    18061 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    18121 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    18181 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt accgaatacg aagagtacaa
    18241 aaaacgcacg ctgggcgcag acgcgctgga gccgaagcgt atcaagtaca agcgtattgc
    18301 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    18361 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    18421 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    18481 tccgcaaatt cgtagcgggt cacccgtgct gattctttta aaccttgttt aatctcgaca
    18541 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    18601 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    18661 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    18721 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    18781 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    18841 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    18901 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    18961 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    19021 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    19081 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    19141 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    19201 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    19261 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    19321 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    19381 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    19441 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    19501 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    19561 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    19621 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    19681 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    19741 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    19801 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    19861 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    19921 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    19981 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    20041 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    20101 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    20161 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    20221 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    20281 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    20341 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    20401 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    20461 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    20521 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    20581 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    20641 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    20701 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    20761 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    20821 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    20881 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    20941 gttaccgcca atcagaatgg cgcttcctgg caccacgccg ccgcctagta cgcggtcgaa
    21001 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    21061 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    21121 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    21181 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    21241 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    21301 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    21361 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    21421 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    21481 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    21541 gtcgccgatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    21601 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    21661 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    21721 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    21781 cacacggctg tgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    21841 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    21901 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    21961 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    22021 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    22081 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    22141 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    22201 tgaaagaggc agaccaggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    22261 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    22321 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    22381 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    22441 gtggcgctga tgcagggagc gtcatggttt agtcaaaacc accagcgaca acgtaatcca
    22501 cagctggaag aactggcccg caccctggcg cgtcaggtaa cgctgaacgt tgcaccgctg
    22561 atgcgtaccg actcaccgga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    22621 gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcacgttct
    22681 ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc gggcggctat
    22741 tttaaccagc agattgtcga accaattgcc gggaaaaacg gaccgctcgg ctatctgcgc
    22801 ctgacgctcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    22861 attttacgcc tgatgttgct gctttcactg gcaatcggcg tagtgctgac ccgcacgctg
    22921 ctacagggta aacgtacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    22981 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    23041 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    23101 gtggaggagt gtatttttcg ccagatgccc gccacgcagc gcgttctgtt tctctggcgc
    23161 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    23221 cggatggaag aagataacgt ccgcctggca cggcgcagta gcggtggcgg cgcggtgttc
    23281 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    23341 atctccacgt cgattgtgct caatgcgctg aacgcgcttg gcgtcagcgc cgaagcgtcc
    23401 gggcgtaacg atctggtggt gaaaactgcc gaaggtgacc gcaaagtctc aggatcggcc
    23461 tatcgcgaaa ccaaagatcg tggcttccac cacggcacct tgctgctcaa tgccgacctt
    23521 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    23581 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    23641 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    23701 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    23761 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    23821 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    23881 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    23941 ggcggcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    24001 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    24061 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    24121 gattatcaat ataatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    24181 taaattatcg cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    24241 gatcgaaaag acggaacggt ttagctcaaa catcaaaagc aaaatggcag aaatttatgg
    24301 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    24361 ttcgtgaata gatacttgaa tagattttat tttctatcca tatgaaaaat aatagtaaat
    24421 ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc tcccactccg
    24481 gcttcacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc cagcagaacg
    24541 gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc ggcagtggtc
    24601 tgctcgtgag tgcggatgtg gtcagacacg gtacagatgg tcagggcttt cgcgccaaat
    24661 tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat gccgtatttt
    24721 tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga gaacaggtta
    24781 ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg caccatgtca
    24841 aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac tttggaatcg
    24901 gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca
    24961 caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag
    25021 atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg gcctttataa
    25081 gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa
    25141 gtttcagcaa tatactttgc acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa
    25201 tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc tacatgtttt
    25261 caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc attatcagaa
    25321 catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag tctggccgat
    25381 atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc cgtaaaccag
    25441 taccggaatg tgttcacgcg tgtgatcagt accggtccag gtcggatcac aaccgtggtc
    25501 agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg gcagacggcg
    25561 gtcgaacagt tccagacccg ccgcataacc tgcgacgtcg cgacggtggc cccaggaaga
    25621 gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt tcatctcttt
    25681 gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg tgataccgca
    25741 gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac
    25801 cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt
    25861 acgctggaag ttaccggctt tgtcgccaat aaacggacga gcgataacac gaccgatgtt
    25921 gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga gtttatccaa
    25981 gccaaaggtt tcttcgtggc aggcaatctg gaacacggag tcagcggagg tatagaaaat
    26041 cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg taccggaaga
    26101 gtggcagtta ccgagataac caggcaggtt agcgcgttca accagtttat ccagcagctc
    26161 ttgcgggaag ctgttttcgt gatcggagaa gtatccccac tcaaacagaa ccgggacacc
    26221 ggcgatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt gcgcccatgc
    26281 gtacgcgccg ataacttcag cgttgccgtc cattcctgcc ggaatgaaac cggtagaacc
    26341 ttcatgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg ggcctttacg
    26401 accgttatca gcttcgcctt tggcacaggc ttctgcgata tgacccaggg tgtcagcccc
    26461 gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt ccagcaccat
    26521 aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa tgatcagatc
    26581 agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg gtgctttatc
    26641 ggcaagttta attgccgctt tcaccgcttt cgccgcgtcc tgccagctgt tttcgtcttt
    26701 cgcgtggata acagccagcg gacgctgacc gtctacctgg tcgcccaaac gcgccatatc
    26761 agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc cgcccattgc
    26821 aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt cggtatcagc
    26881 atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct caacgaagtc
    26941 ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt taccgttgtc
    27001 cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat
    27061 aagcatctcc acgcacagtg ccatcgtgac atcaaacaga cgcgggttac gatattcacc
    27121 cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg aggccagtac
    27181 ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac
    27241 aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc cgctacccac
    27301 tttcacgtcc atcaccagcg catccagacc ttccgccagt ttcttcgcca ggatcgaggc
    27361 agtgatcagc gggatggagt ccacggttgc ggtaatatcg cgggtcgcgt agaaacgttt
    27421 atctgccgga gccaatgagc tggtctggcc gataatcgcc acgccgacgt ctttaataat
    27481 ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc
    27541 gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat agccgccgca
    27601 ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccgc cggtcgagtg
    27661 tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg ttcctgaatc
    27721 tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga agaaaatggt
    27781 catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatac cgttgataaa
    27841 gaaacgaatt tcttcatcgc tcagcgcatg accatcacgt tttttacgaa taatttcttg
    27901 tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg ccggatgcgt
    27961 ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc gcagcgcatc
    28021 aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcgccgt ggcccagagc
    28081 cttcagcagg cttgccagca ggctggaagc accaaagcgg tagtggcgcg catcggccca
    28141 gtcagcgccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg
    28201 cacgccgccc gccggtttga aaccaacggt tttttctacg cccatatcac gaatcacttc
    28261 catcatgatg cgcgcgcttt ccggcgtcgc gttcactgcc actttaccgg tagaggtttt
    28321 gatgaagtca gcacccgctt tgatggagat ttcagacgct ttacggatca gcgcttcgtc
    28381 tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc
    28441 tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca gcgcacggta
    28501 cgggaacacc acgtcaactt catcggcacc gtaggcgatt gccgcacggg tttctgccag
    28561 cgcgatttcg atgtcgtcgt taccgtgtgg gaagttagtt accgtagcaa tacggatttc
    28621 cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag gatagataca
    28681 gatagcggcg gtattgccga ccggagtttt ggcctgatga cacagagcaa ttactttctc
    28741 gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac gcaggctgct
    28801 tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc accttgcgag
    28861 tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac aattaaggaa
    28921 atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc actttcaaat
    28981 gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa acttcttaca
    29041 atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat gaaaatgcac
    29101 tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag cagaagcgcc
    29161 attttctggc tctggaagca gaaaacaatc tgccttatcc gcagcttcct gccgaagccc
    29221 gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac
    29281 cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa tggctggagc
    29341 tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt taccaccacg
    29401 taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg ttgcaaccgt
    29461 atgttagaat tctaacacaa gacgagatcg atattcgaat aaaacgtttc tggcgttacc
    29521 tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct gattcgccca
    29581 ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg aacctgacct
    29641 ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg aagaacatct
    29701 gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt ttcacaaaag
    29761 ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt ggcagcacgc
    29821 tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat gacttcttta
    29881 cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg cggtgtgaat
    29941 tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa gggctgatta
    30001 accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg gttaacttgc
    30061 tgtgtgagaa agaagggatt gccgcacgtt acggtaaaga agccgccgca aatgaagtgg
    30121 gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg aaatatggct
    30181 ggcaaaaacg cgccatgtta cacgcacagt cggggatcag ttccgatatc ggcaccacgc
    30241 cgggcgcgcg tttgccgtat ggcgatgagc cagatccgat cacccatctg caaactgtcg
    30301 ccccgcatca tgcttattat tattccggca tcagcgacat tctgaccctc gacgaaacca
    30361 tcaaacgtaa cccgcaggca ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc
    30421 gtgaatttac cgccaatgtc agcggtaacg atctggttcg cgttaccggt tatatggtgc
    30481 gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc acctggctgg
    30541 gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg ataagccatg
    30601 aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga cgggccaggc
    30661 agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg tcacaatccg
    30721 tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg
    30781 ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tctgcgagca atgtgatacc
    30841 tgcctgaaga ggtgtccgca acatgccacg ccgatggcgc aatccatgag cgtggacgaa
    30901 gtgcttagcc atgtccgcaa agcggtgctg tttatcgaag ggataacggt gagtggcggc
    30961 gaagccacga cccagctgcc gtttgtggtg gcgctgttta ctgctatcaa aaatgatccg
    31021 caactgcgcc atctcacctg tctggtggac agtaacggta tgttgagcga gaccggctgg
    31081 gaaaaattgc tcccggtgtg cgacggcgca atgctcgatc tcaaagcatg gggtagcgaa
    31141 tgtcatcaac aaataaccgg acgcgataat cagcagatta agcgcagcat ctgtttgctg
    31201 gcagagcgcg gtaagctggc ggaactgcgt ttgctggtga tccctggcca ggtggattat
    31261 ttgcaacaca tcgaagaact ggcggcgttt atcaagggac tcggcgatgt tccggtacgc
    31321 ctgaacgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg
    31381 ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag ccggttgata
    31441 tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac agacttgtag
    31501 gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc ggatgcggcg
    31561 tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa gacgcgtcag
    31621 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    31681 cggttcggca caaacctgta ggcatgataa gacgcgtcaa gcgtcgcatc aggcattgtg
    31741 cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaacctgt
    31801 aggcatgata agacgcgaca agcgtcgcat caggcattat gcgccgactg ccggatgctg
    31861 cctacgaagg gtaaactacc cacgaacgct aaacaccgca tacgtattat taagcaacac
    31921 ttcggcaatc tcatcttctg gttccggacg taactcgcaa agcacgtcaa acacccgcac
    31981 agcctgttcc gggcggttag gcttcccctg aaagccgttg agcggcatat ccggcgcgtc
    32041 ggtttccagc aacaacgatg ccagcggtaa ttttgcgatg acatcgcggg ttttactggc
    32101 gcgtggatag gtgatagtac cgcctacgcc gattttgtag cccagctgta caaaccgctc
    32161 ggcctgttgc aggctgccgg aaaaaccgtg aaccacgcca gtgcgcgata aatcgtggcg
    32221 tttaagatgc atcgccagtt tgtcgtgtgt gcgccgtgaa tgcaggatca ccggcagatc
    32281 gtagcgtttc gccagtttca gctgttcgtc gagtaaccac tgctgcctct caagttgcgg
    32341 atcgtcgccg aagagatcca gaccgacctc ccccactgcc accaccttcg ccggacgcct
    32401 ttccagcgcc tgctgtagct gctcaagaga cacatcgcta tgtttttcca acatacctgg
    32461 atgcaagccc aatgcggcat acagcggttg atatttttcc gctaacgcct gcacacgggc
    32521 aaaattttcc gcctctgtcg ccggaacaat gatcttgcct acgcccgctt gtgccgcgcg
    32581 ttgcaggctg gcctcttcat cgccactaaa cggcgggaaa tcaaaatggc agtgggtgtc
    32641 gataaaacgg caaatcaagc cagatcctca ttgttaaatg tcgggtcgtt cgcctgcgga
    32701 gcgtcaatca cttcggcaac cagcgtatcg ttggcgactg gcgcaggcgg aataacgatc
    32761 gattccggcg tcaccactgg caccagatgg cgggtaagcg gcgctttttc agttaatagc
    32821 ttgccaaccg tggcgaggaa ataacgaccg cataaacgcc caagtttata gtcttcacgc
    32881 agcgccggaa tccgactgcc aagcgcgata ctatgtaatg gcttcggcgg ataaatttcg
    32941 aatatccgca gcttgcccgg tggtttctca ataaattgct gaatgtcacg atagctggtt
    33001 tcatgatgct gcaccagatt gaccagcggc tgcaggctac tgtcacccag ccagcgttcc
    33061 atgcgtttga accactgcgg cgtgtagtac atttgtgacg gcacagtgcg aatgacgacc
    33121 aacgttttag cgccctgcct tgccgcctct ttaaccggaa tcgcatcact gatcccgcca
    33181 tccaggtagt taatgccttc cagcgacact ccgctacgat aaaagccagg tatcgcactg
    33241 gaggcgcgaa tcacatccag ccagttttgt ttggttggta aaaagtaatt aggcgcgtag
    33301 tcatcctgac gacaggcgca catataaaac gatttgccgc tgtcaaacaa ccgcgcggcg
    33361 gtatccattt gcaacggcat ctggcttgcg gtggcctcca ccagccagtc gagatcgata
    33421 agatttcctc cacggacaaa gcgcaatgga tcgaaaaatt cgcgttttgt ggtatagcgc
    33481 atgatgactt tgcgcgcgta accgggctga ttgcagataa acgccgagag gttctgcgcc
    33541 ccggcagatg tgccgagata aagatcgaaa ggattaaact gcgcgcgcat aaactcatcc
    33601 agcacgccag ccgtgaaaat tccacgctgt ccgccgcctt cgcacaccag agctattcgt
    33661 ccaggctgga acggccttag cgacaacggc gcaatattac caagcgttac aggtattcgc
    33721 tgccccacct ctgccttcct gtttttgttg tgaaagaatg acaaagtaac gcaatttatt
    33781 attcgctaaa accgcaaaag ccagtccgct ggactggctt tggataaaat tttcttaatg
    33841 gtctgaaatc tagggtcgtt ttcggcccat gaacaaactc accaggaaca gaataatccc
    33901 gacgacaaag acaattttag ctgcgcctgc agcggtaccg gccagaccac caaacccaag
    33961 tgcggcggcg attaacgcga taaccagaaa tatgatgccc caacgaaaca taagcgtctc
    34021 ctttaccata gttaatgtca ccgcttgata tgagcgtgaa aatcactcaa tgcgatattt
    34081 ttagtgtggt gcacatcgcg cctcccgaca aagttcggga ggacgaatta cgacgaatta
    34141 cttagttttc agatcatttt taacgctttt cacaccatct accgctttgg cgatactttc
    34201 agcacggtcg ctctgtgcct gggaatcgac ggtaccggag agctgaacca cgccgtcggt
    34261 ggtttcaact ttcacatgac gggaagggac gatatcgtcc gccagcagtt tggctttgat
    34321 ttcactggtg gtggcggtgt cacccgcgta gcccttcaca gagccttctt tagcgtcgcg
    34381 aacgtgcagt ttgtcgctga cagaggtcac cccttcaacg cctttcgcca ctttcactgc
    34441 ctcttcggcc tgagcctggc tttcaacgaa accgctcagg gtcacgactt tttgatcggt
    34501 tttgacagag atatcggtgc tcttgatgtt gtcatgatcc accagcgccg ccttcacttt
    34561 cgcggtgatg gcgctgtcat ccatgaaatt accgacttta ttcatagagc tatcgacttt
    34621 ttgccctgcg ctttcattgg tagtctgtgc gttgttttcc gcgtaggcag agccggtcgc
    34681 gacggcagag gtcaacatta cagccagcag agttttcgaa atcttcagtc ttgtcatagt
    34741 catcgattta ttcctgtatg tttgctcgta atttgagcct ggcaacacga ggttgcattg
    34801 ctgaataggg agagacttca ccctctacag aagtcaatgg tcgccatcac aaaagcgatg
    34861 ggtgatgaat aacgaccatt acagcctctg aatcagttat taatatcggt agaatgacaa
    34921 tcgacggctt tagatactga tatctacgca ttgaacggta tttaacgccg tcagaaatgt
    34981 cattactttg ttaaatatag atcacaattt tgaaaccgct cgggatatca gcgaaaacat
    35041 aagcaaaagt gaatgtttta agaacattcc gtaagcggct aataaggaag ggaaattgac
    35101 agggcgcagc ggctaccgcg ccctggaggc aagaattaat gctcgcgggt ctggtggaac
    35161 tgaacgtcgg gataacgttc ctgcgccagg cgcaggttga ccatgctggt agcgatgtaa
    35221 gcgaggttat cgccgccatc aagcgccagt tggctttcgt tcttacgctt gaactcttcg
    35281 aatttcttcg cgtccgcaca ttctacccag cgggcagtgg caacgttgac tgattcatat
    35341 actgcttcaa cgttgtattc gctcttcagg cgcgatacca ccacatcaaa ctgcagcaca
    35401 ccaacagcac caacgatcaa atcgttgttg gagatcggac ggaacacctg caccgcgccc
    35461 tcttcggaaa gctgtaccag ccctttgagc agctgttttt gcttcagcgg atctttcagg
    35521 cggatacgac ggaacagttc tggtgcgaag ttcggaatac cggtgaactt catcatctca
    35581 ccctgggtaa aggtgtcgcc gatctgaata gtgccgtggt tgtgcaggcc gaggatatcg
    35641 cccggatacg cttcttcaac gtgcgaacgg tcgcccgcca taaaggtcag cgcgtcggag
    35701 ataaccacat ctttcgcagt acgcacctgg cgcagcttca tgcctttttc atatttaccg
    35761 gatactacgc gcataaacgc cacgcggtcg cggtgtttcg ggtccatgtt ggcctgaatt
    35821 ttaaatacga agccggtaaa tttgtcttcg ctcgcttgta cggtacgggt atcagtctga
    35881 cgcggcatcg gcgcaggtgc ccactccacc aggccatcca acatatgatc gacgccgaag
    35941 ttacccagcg cagtaccgaa gaataccgga gtgatttcgc ccgcaaggaa cagctctttg
    36001 tcgaactcgt tagacgcgcc tttaaccagt tccagttcgt cacgcagctg ctgtgccaga
    36061 tcttcaccaa ccgcagcatc gagatccggg ttattcagcc ctttaacaat gcggacttcc
    36121 tgaatggtgt gccctttacc gctctgatag agataggttt catctttata aaggtggtaa
    36181 acgcctttaa acagcttgcc gcagccaatt ggccaggtga tcggtgcgca gccaattttc
    36241 agctcgttct caacttcatc gagcaattcc atcgggtcgc ggatatcacg gtcaagtttg
    36301 ttcataaagg tgaggatcgg cgtgtcgcgc agacgggtaa cttccatcag cttacgggta
    36361 cgatcttcaa caccttttgc ggcgtcgata accatcaggc agcagtccac cgccgtcagg
    36421 gtacgatagg tatcttccga gaagtcttca tgccccgggg tgtcgagcag gttaaccagg
    36481 caatcgtgat acggaaactg catcacagac gtagtaatgg agatcccacg ctgcttttcc
    36541 atctccatcc agtccgactt agcgtgctgg ttggaaccac ggccttttac tgtaccggcg
    36601 gtctgaatgg cctgtccgaa cagcagcacc ttctcggtga tggtagtctt accggcgtcc
    36661 gggtgagaaa taatggcaaa agtgcggcgc ttcgccacct cttgcaaata aggagacaac
    36721 gtcataattc tttcttcttg agtaagcggc atcgcgccgc gcttgttgaa aacgaaaaat
    36781 tgcggctatt ttacccatca acggggggga ggcaatcagt gtttacacag gagctgctcc
    36841 agttcatgca acgaagaaac ggtccaggtg ggcgcgatgc cttctggttg ctcgcgatgg
    36901 tgtgcattca gccagcaggt cgcaagcccg gcgttgatgc caccgagaat atcggactcg
    36961 gcagtgtcgc caaccatcag cacgcgtgaa cggtcaggat tgcccgcctg ttccagcgca
    37021 taatcgaata ttttcttatt cggtttagca acgccaactt cttcggaaat caccagcaaa
    37081 tcgaagtaat cacgcaggcc cgtccgttcc agacgcactt gctgcaaggc gctaaaaccg
    37141 ttggtgatga tgccgatttt agcgttgcca cgaatggcgt taagcagaga aaccgcgccc
    37201 ggcaacggcg tacagatttc cgccatcgca ttaataaagg catcgttaag caagcctgct
    37261 tcaactttca gccgttcggc ccagctctca aaccgcccgt gctgaagctg taatgaagtg
    37321 atcgcgccgt tttgataatc cacccacagt ggcttgttaa cggcctgata gtcctgaaaa
    37381 tcttcagcgg taaaggtgac gctgtaatca agaaacatcc gctgcaggcc ggtgaatgag
    37441 tcaaaggtaa acagcgtttc atcggcatca aagaaaatcc agtcccactt cattattcca
    37501 ccttgtctta catactgatt ggcaacgcca tgatgatggc gtcttcgcga ccgtccgtgg
    37561 tggggtagta attgcggcga atcgtcgcct cgttaaagcc taaactttcg tacagggcaa
    37621 tggcggcagc gtttgaagca cggacttcca gccatagtgt cgccacgccg cgtttttcca
    37681 gttcgtcgat cagatgttcc agcagcaccc ttcccaatcc ctgacgctga tagtcaggat
    37741 cgaccgcaat attgaacaat gtagcttcat ccagcaccac ttgcgtaatc gcaaacgccg
    37801 ccattttgcc gttttgcgtt aactgaaagt tgagataacg ctcgccctgg ttgctggcaa
    37861 aggttttttc actccacgga aaggcgtggg cgcgttgttc aatgtggtaa gccgctggta
    37921 aatcagtcgt ttcgagggaa gaaatcgtgt tcatatgtgc aaatctgttg ccataacgcg
    37981 gcgcgtgccg ttgggtttgc ccgtaattcg gtgagcgccg gtgatgccac ctgagcgcct
    38041 tccagtgata gcggttcgtc agtacccaac cgccagctgt tgcagcgact gccttgcggc
    38101 agcatcgcga ttttttctgg cgtcagttgc agcacttgat cggggctgac ggttaatgcg
    38161 cgcagaacat cgctcactaa aggatcggtc agggcgggaa gatcgtttgc caccatcacc
    38221 agacggacgt gtgccgggat cgcaatggca atctccccct gcaacgcgcc agggcgacgc
    38281 agcgaccact gggtaatgcc cagttgctgt aactgccagt ctcgtcggga tgtcatagcg
    38341 aatcgctcct gttgtcaggg gcgcaaatat agcaaattcg tcgataccgc gccaacatat
    38401 ggctataatc gccgccagta tcaattgagg agcattccat gtctgcattt accccggcaa
    38461 gtgaagtctt gctgcgtcac agtgatgatt tcgaacaaag ccgtattctg tttgccggag
    38521 acttacagga tgacctgccc gcgcgtttag ataccgcggc cagccgtgct catacccagc
    38581 aattccacca ctggcaggta ttaagccgcc agatggggga taacgcccgt ttcagtctgg
    38641 tcgccacggt ggatgacgtc gcagattgcg atacgctgat ttactactgg ccgaagaaca
    38701 aaccggaagc ccagttccag ttgatgaatt tactttctct gctgccagtg gggacagata
    38761 tttttgtcgt tggcgagaac cgcagcggcg tgcgcagcgc cgagcagatg ctggcagatt
    38821 atgcgccgtt gaataaagtc gacagcgctc gtcgctgtgg cctctatttt ggtcgtctgg
    38881 aaaaacagcc ggtatttgat gccgataaat tctggggcga atacagcgtc gatggcctga
    38941 cggtcaaaac gctgcctggc gtgtttagcc gcgacggtct ggatgtcggt agccagttgc
    39001 tgctctcgac gttaaccccg cacacgaaag gtaaagtgct ggatgtcggc tgtggcgcgg
    39061 gtgtgctttc agttgccttt gcgcgccatt cgccgaaaat tcgtctcacc ttgtgcgatg
    39121 tctctgcgcc agcggtagaa gccagccgcg caacacttgc ggccaacggt gttgaaggtg
    39181 aagtctttgc cagcaacgtc ttttccgagg tgaaaggtcg ttttgatatg atcatctcca
    39241 acccgccgtt ccacgatggg atgcaaacca gcctggatgc ggcgcaaacg ctgattcgcg
    39301 gcgcggtgcg tcatcttaat agcggcggcg agctgcgaat tgtagcgaac gccttcctgc
    39361 cttacccgga cgtgctggat gagacatttg gcttccatga agtgattgcg caaaccgggc
    39421 gtttcaaggt gtatcgcgcc attatgaccc gccaggcgaa gaaaggttaa ttatctcgcc
    39481 gaataccgtg tcggatgcgg cgtgaacgcc ttatccgacc tacaaaacct gcacgttagc
    39541 ccttcgtagg ccagataaga cgcgccagcg tcgcatctgg catctgcccc cgcatgccta
    39601 ccaaacctgc acgctagttt cctgatggac atttttccag caattacacc tccggcgata
    39661 attaactatt gacgaaaagc tgaaaaccac tagaatgcgc ctccgtggta gcaattcttt
    39721 ttaagaattg atggtatgcg aaggtggcgg aattggtaga cgcgctagct tcaggtgtta
    39781 gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaaaaacc acgttgatat
    39841 tgctcgcact gggcgaaggt ggcggaattg gtaga
//
LOCUS       DAZFWP020000024        39681 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFWP020000024 DAZFWP020000000
VERSION     DAZFWP020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.024"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            156..599
                     /gene="cmtB"
                     /locus_tag="ACHK0Z_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             156..599
                     /gene="cmtB"
                     /locus_tag="ACHK0Z_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU4501213.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            627..2015
                     /gene="cmtA"
                     /locus_tag="ACHK0Z_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             627..2015
                     /gene="cmtA"
                     /locus_tag="ACHK0Z_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU4501214.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNLGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            2030..3307
                     /gene="yggP"
                     /locus_tag="ACHK0Z_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             2030..3307
                     /gene="yggP"
                     /locus_tag="ACHK0Z_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4501215.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3304..4269
                     /gene="glpX"
                     /locus_tag="ACHK0Z_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             3304..4269
                     /gene="glpX"
                     /locus_tag="ACHK0Z_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4501216.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDTYMKKLVVNRLAAGAIDLSLPLADNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4291..4800
                     /gene="fumE"
                     /locus_tag="ACHK0Z_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             4291..4800
                     /gene="fumE"
                     /locus_tag="ACHK0Z_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU4501217.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4797..5510
                     /locus_tag="ACHK0Z_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             4797..5510
                     /locus_tag="ACHK0Z_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU4501218.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5482..6152)
                     /locus_tag="ACHK0Z_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /pseudo
     CDS             complement(5482..6152)
                     /locus_tag="ACHK0Z_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(6146..6823)
                     /locus_tag="ACHK0Z_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             complement(6146..6823)
                     /locus_tag="ACHK0Z_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4501219.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6811..7518)
                     /locus_tag="ACHK0Z_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             complement(6811..7518)
                     /locus_tag="ACHK0Z_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU4501220.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAIRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7519..8097)
                     /locus_tag="ACHK0Z_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             complement(7519..8097)
                     /locus_tag="ACHK0Z_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU4501221.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8120..8551)
                     /locus_tag="ACHK0Z_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             complement(8120..8551)
                     /locus_tag="ACHK0Z_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU4501222.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8923..9942
                     /gene="epd"
                     /locus_tag="ACHK0Z_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     CDS             8923..9942
                     /gene="epd"
                     /locus_tag="ACHK0Z_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU4501223.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9992..11155
                     /gene="pgk"
                     /locus_tag="ACHK0Z_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     CDS             9992..11155
                     /gene="pgk"
                     /locus_tag="ACHK0Z_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU4501224.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11370..12449
                     /gene="fbaA"
                     /locus_tag="ACHK0Z_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     CDS             11370..12449
                     /gene="fbaA"
                     /locus_tag="ACHK0Z_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4501225.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12513..12611)
                     /locus_tag="ACHK0Z_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /pseudo
     CDS             complement(12513..12611)
                     /locus_tag="ACHK0Z_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12807..13667
                     /gene="mscS"
                     /locus_tag="ACHK0Z_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             12807..13667
                     /gene="mscS"
                     /locus_tag="ACHK0Z_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4501226.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13806..14441
                     /gene="argO"
                     /locus_tag="ACHK0Z_RS05845"
                     /old_locus_tag="pgaptmp_001169"
     CDS             13806..14441
                     /gene="argO"
                     /locus_tag="ACHK0Z_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU4501227.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14534..15274
                     /gene="yggE"
                     /locus_tag="ACHK0Z_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             14534..15274
                     /gene="yggE"
                     /locus_tag="ACHK0Z_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU4501228.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVSDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15441..16337
                     /gene="ygfI"
                     /locus_tag="ACHK0Z_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     CDS             15441..16337
                     /gene="ygfI"
                     /locus_tag="ACHK0Z_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4501229.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMSASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16334..17812)
                     /gene="scpC"
                     /locus_tag="ACHK0Z_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             complement(16334..17812)
                     /gene="scpC"
                     /locus_tag="ACHK0Z_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU4501230.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17836..18621)
                     /gene="scpB"
                     /locus_tag="ACHK0Z_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             complement(17836..18621)
                     /gene="scpB"
                     /locus_tag="ACHK0Z_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU4501231.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHAIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18632..19627)
                     /gene="meaB"
                     /locus_tag="ACHK0Z_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             complement(18632..19627)
                     /gene="meaB"
                     /locus_tag="ACHK0Z_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU4501232.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19620..21764)
                     /gene="scpA"
                     /locus_tag="ACHK0Z_RS05875"
                     /old_locus_tag="pgaptmp_001175"
     CDS             complement(19620..21764)
                     /gene="scpA"
                     /locus_tag="ACHK0Z_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU4501233.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRIVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21968..22861)
                     /gene="argP"
                     /locus_tag="ACHK0Z_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             complement(21968..22861)
                     /gene="argP"
                     /locus_tag="ACHK0Z_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU4501234.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23003..23188
                     /locus_tag="ACHK0Z_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             23003..23188
                     /locus_tag="ACHK0Z_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501235.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23244..23903
                     /gene="rpiA"
                     /locus_tag="ACHK0Z_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             23244..23903
                     /gene="rpiA"
                     /locus_tag="ACHK0Z_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU4501236.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24159..25391
                     /gene="serA"
                     /locus_tag="ACHK0Z_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             24159..25391
                     /gene="serA"
                     /locus_tag="ACHK0Z_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4501237.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25620..25679
                     /gene="ibsC"
                     /locus_tag="ACHK0Z_RS05900"
                     /old_locus_tag="pgaptmp_001180"
     CDS             25620..25679
                     /gene="ibsC"
                     /locus_tag="ACHK0Z_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU4501238.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            26012..26071
                     /locus_tag="ACHK0Z_RS05905"
                     /old_locus_tag="pgaptmp_001181"
     CDS             26012..26071
                     /locus_tag="ACHK0Z_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4501239.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26172..26774)
                     /gene="fau"
                     /locus_tag="ACHK0Z_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             complement(26172..26774)
                     /gene="fau"
                     /locus_tag="ACHK0Z_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU4501240.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26795..26978)
                     /gene="ssrS"
                     /locus_tag="ACHK0Z_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     ncRNA           complement(26795..26978)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK0Z_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27020..27349)
                     /gene="zapA"
                     /locus_tag="ACHK0Z_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             complement(27020..27349)
                     /gene="zapA"
                     /locus_tag="ACHK0Z_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4501241.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27517..28095
                     /gene="ygfB"
                     /locus_tag="ACHK0Z_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             27517..28095
                     /gene="ygfB"
                     /locus_tag="ACHK0Z_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU4501242.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28121..29446
                     /gene="pepP"
                     /locus_tag="ACHK0Z_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             28121..29446
                     /gene="pepP"
                     /locus_tag="ACHK0Z_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU4501243.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29443..30621
                     /gene="ubiH"
                     /locus_tag="ACHK0Z_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /gene_synonym="visB"
     CDS             29443..30621
                     /gene="ubiH"
                     /locus_tag="ACHK0Z_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU4501244.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGETLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30644..31846
                     /gene="ubiI"
                     /locus_tag="ACHK0Z_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /gene_synonym="visC"
     CDS             30644..31846
                     /gene="ubiI"
                     /locus_tag="ACHK0Z_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU4501245.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLVADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31920..32003)
                     /locus_tag="ACHK0Z_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             complement(31920..32003)
                     /locus_tag="ACHK0Z_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501246.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            32293..33387
                     /gene="gcvT"
                     /locus_tag="ACHK0Z_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             32293..33387
                     /gene="gcvT"
                     /locus_tag="ACHK0Z_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU4501247.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33411..33800
                     /gene="gcvH"
                     /locus_tag="ACHK0Z_RS05955"
                     /old_locus_tag="pgaptmp_001191"
     CDS             33411..33800
                     /gene="gcvH"
                     /locus_tag="ACHK0Z_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU4501248.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTFTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33919..36792
                     /gene="gcvP"
                     /locus_tag="ACHK0Z_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             33919..36792
                     /gene="gcvP"
                     /locus_tag="ACHK0Z_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU4501249.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            37053..37796
                     /gene="ygfF"
                     /locus_tag="ACHK0Z_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             37053..37796
                     /gene="ygfF"
                     /locus_tag="ACHK0Z_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4501250.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37853..39286)
                     /gene="bglA"
                     /locus_tag="ACHK0Z_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             complement(37853..39286)
                     /gene="bglA"
                     /locus_tag="ACHK0Z_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU4501251.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39331..39642
                     /gene="yqfB"
                     /locus_tag="ACHK0Z_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             39331..39642
                     /gene="yqfB"
                     /locus_tag="ACHK0Z_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU4501252.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
BASE COUNT         9495 a         9906 c        10679 g         9601 t
ORIGIN      
        1 tttgttgtta tatctataac tcaatgttat ataagaaatt aataattcac tgttttcaaa
       61 acaccggttt ccctgctcaa ttgctttcat taaaccgcac gagtatttta tttttaccgc
      121 cggacggtac cctaaaaata aaataaggat aatttatgcg gcttagtgac tattttccag
      181 aatcatcaat ctcagttata cattcagcaa aagattggca ggaagctatc gatttctcga
      241 tggtatcatt gctggataaa aactatatca gcgagaatta cattcaggca attaaagatt
      301 ccaccattaa caatggccct tattatattc tcgcaccagg cgtggcaatg cctcatgcgc
      361 gaccggaatg tggggcgctt aaaaccggga tgtctttgac attacttgaa caaggtgttt
      421 attttccggg gaatgacgaa ccaattaaat tactcatcgg actctctgct gccgatgccg
      481 attcgcacat tggcgctatt caggcgttaa gtgaattact gtgcgaagaa gaaatactcg
      541 aacaactctt aacagcatca tcagaaaaac aattagcgga cattatcagc cgcggataat
      601 tactctctcc tctacaggat aatattatgg aaaacaagtc tgctcgtgca aaggtccagg
      661 cttttggggg ctttttgact gcaatggtca tccccaatat aggtgctttt attgcctggg
      721 gcttcataac tgcgttattt attcccaccg gttggctgcc gaatgaacat ttcgccaaaa
      781 ttgtcggccc gatgattacc tatttattgc ccgtgatgat tggttctact ggtggtcatc
      841 tggtcggcgg taaacgcggg gcggtcatgg gcggaatagg tactattggt gtgatcgttg
      901 gcgcagagat cccgatgttc cttggctcaa tgatcatggg gccgctcggt gggttggtca
      961 taaaatatgt cgataaggca ctggaaaaac gcatacctgc cggttttgag atggttatca
     1021 ataacttctc attaggtatc gcggggatgc tcctttgtct gctgggtttt gaagtgatcg
     1081 gcccggcggt gttaattgcc aatactttcg tcaaagagtg tatcgaggcg ctggtacatg
     1141 cgggttatct gcctctgctg tcagtcatca atgaaccggc gaaagtgctt ttccttaata
     1201 atgcgatcga tcagggcgtc tattatccgc tgggaatgca acaggcttcg gttaacggta
     1261 aatccatctt ctttatggtg gcctctaacc taggtccggg cctggggctg ctgctggcgt
     1321 ttaccttgtt tggtaaaggg atgagtaaac gttctgcgcc cggggcgatg attattcact
     1381 tcctcggtgg gatccacgaa ctgtatttcc catatgtgct gatgaagccg ctgactatta
     1441 ttgccatgat tgcgggcggt atgtctggta cctggatgtt taacttactg gacggtggtc
     1501 tggtggctgg cccaagtccg gggtctatct ttgcttacct ggcactgacg ccgaaaggct
     1561 cgttcctggc gacaattgcc ggtgttacgg taggtaccct ggtgtccttt gctattactt
     1621 cgctgatact gaagatggaa aaaacggtgg aaacggagag cgaagatgag tttgctcagt
     1681 cagccaatgc ggttaaggcg atgaaacaag agggtgcatt ctcgttaagc agggttaagc
     1741 gtatcgcctt tgtttgcgat gcggggatgg gctccagtgc gatgggcgcg accaccttcc
     1801 gtaaacgcct ggaaaaagcg gggctggcaa tagaagtaaa acattacgcg atagaaaacg
     1861 tgcctgcgga tgccgatatc gtcgtcactc acgccagtct ggaagggcgc gtgaaacgtg
     1921 tgacggataa accgctgata ttgattaata actatattgg cgatccaaaa ctcgacactt
     1981 tatttaatca attaaccgcc gaacataaac actgattaca gaggtaaaaa tgaaaaccaa
     2041 agttgctgct atttatggca agcgggatgt ccgtctgcgc gaatttgaac tgccagaaat
     2101 taccgataat gaactgttag tgagtgtaat ttctgacagc gtctgtttat cgacctggaa
     2161 agcggcgtta ctcggtagtg aacataaacg cgtacccgac gatttagaaa atcatccggt
     2221 cattaccggg catgaatgtg ccggggttat tgtcgaagtg ggtaaaaatc tcactggcaa
     2281 atataaaaaa ggccagcgtt ttgtattgca accggcgatg gggttaccaa gcggatattc
     2341 agcaggctac agctacgaat attttggcgg taacgccacc tatatgatta ttcccgaaat
     2401 cgccattaat ttgggctgcg tattaccgta tcacggctct tattttgctg cggcgtcgct
     2461 ggcagagcct atgtgctgca ttattggtgc ttatcatgcc aattatcaca ccacgcaata
     2521 tgtttatgag catcgcatgg gcgtcaaacc tggcggcaat attgcactgc tggcgtgtgc
     2581 gggtccgatg ggcattggcg ctatcgatta cgccattaac ggtggcatac aaccgtcacg
     2641 ggtggtggtg gtcgatatcg acgacaaacg cctggcgcag gtgcagaagc tgctgccggt
     2701 ggatctggcg gccagtaaag gcattgagct ggtgtatgtg aataccaaag ggatgagcga
     2761 tccggtccag acgctgcggg cgctgacagg agatgtcggg ttcgatgaca tttttgttta
     2821 tgcggcggtg cctgctgtcg ttgagatggc tgacgaatta ctggcggaag atggctgtct
     2881 gaacttcttt gccgggccga cggataaaaa cttcaaagtg ccgtttaatt tctacaacgt
     2941 ccattacaac agcacgcacg tagtcggtac atccggcggt tcaacggacg acatgaaaga
     3001 ggcgattgcg ttaagcgcca cggggcagtt gcagccgtcc tttatggtca cgcatatcgg
     3061 tgggctggat gcggtgccag ataccgtgct caatctgccg gatatccctg gcggtaaaaa
     3121 actcatttat aacggcgtga ccatgccgct cactgccatt gccgattttg ccgaaaaagg
     3181 caaaaccgat ccgctgttta aagagttggc gcggctggtt gaggaaacgc acggcatctg
     3241 gaatgaacag gccgagaaat atctgctggc acaatttggc gttgatatcg gggaggccgc
     3301 gcaatgatgt ccctggcgtg gccattattt cgcgttacgg aacaggcagc gttggctgcc
     3361 tggccgcaaa ccggatgtgg cgacaaaaat aaaattgatg gcctggcggt caccgcgatg
     3421 cgccaggcat taaacgacgt cgcttttcgt ggacgagtgg ttatcggcga aggagaaatt
     3481 gaccatgcgc cgatgctgtg gattggcgaa gaggtgggca aaggcgatgg gccagaagtc
     3541 gatatcgcgg ttgacccgat tgaaggcacg cggatggtag cgatggggca gagcaatgcg
     3601 ctggcggtca tggctttcgc ccctcgagat agcctgttac atgcgcccga tacgtatatg
     3661 aaaaagctgg ttgttaatcg actggcggcg ggcgcgattg atctgtcttt gcctctggcg
     3721 gacaacttgc gcaacgtggc gaaagcgtta ggtaaaccgc tggacaagct gcgcatggtt
     3781 acgctggata aaccgcgcct gagtgccgcg attgaagaag caacacagct gggcgtgaag
     3841 gttttcgctc tgccggatgg cgatgtcgcc gccagcgtgc tgacctgctg gcaggataat
     3901 ccttacgacg tgatgtacac catcggcggt gcgccagaag gcgtgatttc tgcctgtgcc
     3961 gttaaagcat taggtgggga tatgcaggcc gagctgattg acttttgtca ggctaaaggg
     4021 gattacacgg aaaatcggca gattgcagag caggagcgca agcgttgtaa ggcaatgggc
     4081 gttgacgtta accgtgtgta ctcgctcgat gaactggtga gggggaacga tatcctcttt
     4141 agcgctacgg gcgtgacggg cggcgagttg gtgaacggta tccaacagac ggcgaatggg
     4201 gtgcggacgc agacattact gatcggcggc gcggaccaaa cgtgtaatat aatagactcc
     4261 ctgcattgat ggtgatctat cggaagacca atggcgacgc tgacagaaga tgatgtgctt
     4321 gagcaactgg atgcacagga caatttattt tcatttatga aaactgcgca ttctattttg
     4381 ctccagggga tacgccagtt tctgccgtcg ctgtttgtcg ataacgatga agagatcgtc
     4441 gaatatgcag tgaagccgtt actcgcccaa agcggcccgc ttgacgatat tgatgttgcg
     4501 ctgcgtttga tttatgcgct ggggaaaatg gataaatggc tgtatgccga tatcacgcac
     4561 ttttcccagt actggcatta cctgaacgaa caggatgaaa cgcccggatt tgccgatgac
     4621 atgacctggg attttatcag caacgtcaat agcataacct gcaatgcgac gctctatgac
     4681 gcgttaaaag cgatgaagtt cgccgatttc gccgtctggt cagaggcgcg ttttagcgga
     4741 atggtcaaaa cggcgctgac gctggcagta acgaccacct taaaggaatt aacgccgtga
     4801 aaattgaatt aacggtgaat gggctgaacg tgcaagcgca gtaccatgat gaagagatag
     4861 aacgcgtgca taaaccgttg ctgcgtatgc tggcggcctt acagacggta agtccgcagc
     4921 gacgtacggt ggtttttctt tgcgctccgc cggggacggg taaatctacc ttaactacct
     4981 tctgggaata tctcgcgcaa caagaccctg aattacccgc tatccagacg ctcccgatgg
     5041 atggttttca ccattacaat agctggctgg atgcgcatca attgcgcccc ttcaaaggcg
     5101 caccagagac atttgacgtt gcgaaactgg cggaaaatct gtgccaggta gtggaagggg
     5161 attgtacgtg gccgcagtat gatcgacaaa agcatgatcc tgttgaagat gcgttgcacg
     5221 ttaccgcacc actcgtcatc gtcgaaggaa actggttgtt gctggacgac gagaagtggt
     5281 gccagcttgc gcaattttgt gatttctcga tctttatcaa cgcgccagct actgcgttgc
     5341 gggaacgact ggtagggcgc aagctggcgg gaggggtatc gctggcagac gcagaagctt
     5401 tttatgaccg taccgacggg ccgaatgttc gccgggtgct ggaagagagt ctgcctgcaa
     5461 atttgacctt aatgatgacc gctacgggtg aatatcgtct ggtggattaa cgttcctgat
     5521 tagaccatct tccagtcgca ccacacgaga gaaatgacgc cgggtaaatg cggcgtcatg
     5581 actgattgct acgacgctcg tgcctcgctg gccgcacttc tccagccagc tttcaaacac
     5641 gctcaaccaa ttttcgtcaa aatcccgact cggctcgtcc agcagcaata gtggcggaga
     5701 gagtgcttcc aggcaggcga cggcgaccat ccgtcgctgc gcactatgca gatccaacgg
     5761 gtgcgagttg gcagtgtctg ctaactgaca acattgccat gggtacgttg agtgatttca
     5821 tccgccgggc atttttgcag tttcaaacca aatgcgatct cgtcagcgac ggtgctgtga
     5881 aaaagttggt tttccgcttc ctgaaacagt acgccaacct ttgccgcgcg ctggcggttt
     5941 ttcaggtttt tcatcgcctg ttgttgcagc ataaccgtac cggctgtggg cctaagaaga
     6001 cctgccatta ctcgtaacag cgtcgatttc cctgcgccat tgtcaccggt cagcgccaac
     6061 cattcacctt gtttgagttg cagagaaatg tcgcaaagac aatccgttgc cgcgccaggc
     6121 cagcgataag atatcttatt taacgttaac atagtggctg gagcgctccg ttttgcaatt
     6181 gccacgcttg ctggcagtag ttcaggaaag gtgtgcgatg acgttcaaaa agaataatca
     6241 gcgaaccacg ttcaaacgcc cagtgttgta agcgctgcaa cagcatttcg ctggcctgcg
     6301 gagtcaggcg gctaaaggct tcatcaagaa ttaagagctt tggctgcatc gcgatggcgc
     6361 aggcgatgac cacgcgctgt gtctctccac cggagagtgt tgccgggtga cgatggcgca
     6421 acggctggca ctcggtcaga gtcagggcgg catcaatccg cgccataatc tctgcttcag
     6481 caagacacag attttctggc ccaaaggcaa cttcttcttc cacgctaaag gtacagccgg
     6541 agagctgcaa atagggcgat tgctggacga gctggatggt ggcggattgt tcattaagcg
     6601 ggagatgccc aatgggcgta ccgaggagcg tccccgtgcc tgttatctct cctggcagaa
     6661 agtccggata ccagcccgcc atcagctgtg ccagcgtact tttgccactg ccgttgtcac
     6721 caaagatggc gaccatcccc ggtttgacat agtgaaagtc ataacagaac ggaggatgcg
     6781 ttgagtgtgt cgggcaataa cggaactgtt ctaacgtaac catatccacg ctccgaattc
     6841 tgtcagcatc agcaagatca tggtgtaacg cgctacgcgt tgtagggtgc tatcggctgg
     6901 tgcccacaac gttgttcgtc ggttgtggat ccgaaatgcc cgcatatcca gcgccgcacc
     6961 acggatagcc agatcgttta gcgcattgtg tgttagcgga ataattaacg cgggcatcgc
     7021 acgtaaacgc tgataccagc cctcatccag cggcacgcca cgggcacgct gcgcttcatg
     7081 aataatcgct aattgccgtt ttaactgctc aacgaccaat aacggcccgg caaacagata
     7141 agcgacgcct ggcggcaggc gagaagcaaa cagagcgcga ataaaacatt gcacggggac
     7201 gtactgcatc cacaactgtg aggttgaaac aatcgccaga atccgcaacc agagcgtaat
     7261 agcatgcgac caccgttctg gcgagcgtgg cgtgccgctt agccattctg gtagccagcc
     7321 accgtgtacc agccacagcc cggctcccag cgagaacatc agccagacga catattttgc
     7381 ccgccgtcgc gtggccttta gcgcaataag gcagaaaaag gtcgccgcgc tatagatcgg
     7441 caaaatggtc tgcgcgggca ggataagcgt ggtgcaggcc gccaatgccc acagtgttaa
     7501 tgaggtaaat ggatgcattt agcgcacggc agccattgcc ggaaaatggc gcgtggtgcg
     7561 aatcggcagc tggcgcagta aaagccagac aatcaccgct gtaagaattt tatcgaccag
     7621 gttcgcgcct atcaccgtaa tcgccaccga ctccaccagg ttttgcccca tcgagtgcat
     7681 ccaggcaacg aacaaatcgg ctccgctacc ggtgacaccg ccgaacagcg cagtacgcag
     7741 cggtacagcg acgaccgtta ccgcaagcgt aataatcacg ccgctgacaa cgacttttgg
     7801 cagggtgcga aaccaacccg ctcgcgccag ccaaccggcg accaggccga tgaccatcgc
     7861 gacgggtgca aatgcggcgg cgatcgggtc ggtcagcaat ccccagagta aattggtcag
     7921 taagccggtc agcattccga tcaccggacc aagcagtacc gcgctaataa gtgtgccgat
     7981 ggagtcgaga aatataggta atttgaccat gctggccagt tgcccgccga tcatattgat
     8041 ggcgatagat ataacgatca acaccagtgc ctgactggaa aagtggctac gcgccataag
     8101 aaaacctctt tgaaagtagt tatttaacgg gggaaatatg caactcgtcg taaccagaaa
     8161 gcgcgcaagg ttggcggcta aacgccagtt cctcaaggct accgatcacc agttcaagcg
     8221 tggtccgcac ggtacagccg ggcatcatat gaccgccaag catcgtgccg tgtggatcgg
     8281 aaacgcagag atgaagatgc tcaccactct gttccagcgt gccgtttagt gagatcactt
     8341 cgaatgtacc gttcaggagc gtggtgcctt cttgcccggc atagcgcaag gcgacgtcag
     8401 tcaggctacc ggtacagcct gcaatccagg cggcatgaag ttgatgctgc tgtgcaaaag
     8461 cgtgtagctg ggaaaaaact tcctgcccgg ggaggaggcg cagtgcgtaa aagcgtgcgg
     8521 aggaagaata tgggtagggc aatgtcatca ttggagtact ccgaaactcg gacgtattgc
     8581 ggataatatc gacgttgcat cagtcatatt ctgatataag tcagggtttg tgacggcaca
     8641 acgaaaagat tttgctacgc ttattcgtga ttctgcaaag tggaataaat tccacagggg
     8701 cggggctgaa aacgtgacgt aagtcagata attgctttct ttgactggac aatcattcct
     8761 tttattccac gtttcgctta tcctagctga agcgtttcag tcgattaaat gttcgacaat
     8821 taaccaatca gtcgcagttt gcgacaggta aggtttcccc ggacgatttg ctggattact
     8881 ctgtctggca aatttgctca gggaaaacct tgcaggagat ctatgaccgt acgcgtagcg
     8941 ataaatggct tcggtcgcat cgggcgtaat gtggttcgtg ctttgtatga atccggacgc
     9001 cgggcggaaa ttaccgtggt ggcaatcaac gaactggcgg atgctgcggg catggcgcat
     9061 ttgttgaaat atgacaccag ccatggccgt tttgcatggg aagtacgaca ggaacgcgat
     9121 caactttttg ttggtgatga cgccatccgc gtattgcatg aacgttcact gcaatcgctc
     9181 ccctggcgtg aacttggcgt tgatgtagtc ctcgactgca ccggcgtata tggctcccgc
     9241 gagcatggcg aagcgcatat tgccgccggg gccaaaaaag tgctcttttc acatcctggc
     9301 agtaacgatc tcgacgcgac cgttgtttac ggcgtcaatc aggatcaact tcgtgcggaa
     9361 caccgcatcg tttctaacgc ttcctgtacc acgaattgca taattcccgt catcaaattg
     9421 ttagatgatg cgtacggtat tgagtccggc actgtgacca caattcactc cgccatgcac
     9481 gatcaacagg ttattgatgc ataccatcct gacctgcgtc gcacccgggc agccagccag
     9541 tcgatcattc cggtcgatac taaactggcc gccggtatca cacgattttt tccgcaattt
     9601 aacgatcgct ttgaagcgat tgcggtacgt gtgccaacca taaatgtgac ggcaatcgat
     9661 ttaagcgtga cggtgaagaa acctgtaaaa gccaatgaag tcaacctgtt gctgcaaaaa
     9721 gcagcacaag gtgcatttca tggtatagtt gactatacgg aattgccgtt ggtctctgta
     9781 gattttaacc acgatccgca cagtgccatt gtcgatggca cccaaacccg ggtcagtggc
     9841 gcacacctga tcaaaacgtt ggtctggtgc gataacgaat ggggctttgc taaccgaatg
     9901 ctcgacacga cgttagctat ggctactgtt gctttcaggt aagacgcaag cagcgtctgc
     9961 aaaactttta gaatcaacga gaggattcac catgtctgta attaagatga ccgatctgga
    10021 tcttgctggg aaacgtgtat ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa
    10081 agtaaccagc gacgcgcgta tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca
    10141 aggcgcaaaa gtgatggtaa cttcccacct gggtcgtcct accgaaggcg agtacaacga
    10201 agaattctct ctgctgccgg ttgttaacta cctgaaagac aaactgtcta acccggttcg
    10261 tctggttaaa gattacctcg acggcgttga cgttgctgaa ggtgaactgg ttgttctgga
    10321 aaacgttcgc ttcaacaaag gcgagaagaa agacgacgaa accctgtcca aaaaatacgc
    10381 tgcactgtgt gacgtgttcg taatggacgc attcggtact gctcaccgcg cgcaggcttc
    10441 tactcacggt atcggtaaat tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga
    10501 actggacgcg ctgggtaaag cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg
    10561 tggttctaaa gtatctacca aactgaccgt tctggactcc ctgtctaaaa tcgctgacca
    10621 gctgattgtt ggtggtggta tcgctaacac ctttatcgcg gcacaaggcc acgatgtggg
    10681 taaatccctg tacgaagctg acctggttga cgaagctaaa cgtctgctga ccacctgcaa
    10741 catcccggtt ccgtctgatg ttcgcgtagc aaccgagttc tctgaaactg caccggctac
    10801 cctgaaatct gttaacgatg tgaaagctga cgagcagatc ctggatatcg gtgatgcttc
    10861 cgctcaggaa ctggctgaaa tcctgaagaa tgcgaaaacc attctgtgga acggtccggt
    10921 tggcgtgttc gaattcccga acttccgcaa aggtactgaa atcgtggcta acgctatcgc
    10981 agacagcgaa gcgttctcca tcgctggcgg cggcgacact ctggcagcaa tcgacctgtt
    11041 cggcattgct gacaaaatct cctacatctc cactggcggc ggcgcattcc tcgaattcgt
    11101 ggaaggtaaa gtactgcctg cagtagcgat gctcgaagag cgcgctaaga agtaaaaaat
    11161 cacagggcag ggaaacctgc ccttgtttca gcgcgctttt agagcacgca ttttttcata
    11221 ctctaaataa ttcgagttgc aggaaggcga caagagagtg aatccccagg agcttacata
    11281 agtaagtgac tggggtgagc gaacgcagac gcagcacatg caacttgaag tatgatgagt
    11341 ataaggcccg acgatacagg acaagagaca tgtctaagat ttttgatttc gtaaaacctg
    11401 gcgtaatcac tggtgatgac gtacagaaag ttttccaggt agcaaaagaa aacaacttcg
    11461 cactgccagc agtaaactgc gtcggtactg actccatcaa cgccgtactg gaaaccgctg
    11521 ctaaagttaa agcgccggtt atcgttcagt tctccaacgg tggtgcttcc tttatcgctg
    11581 gtaaaggcgt gaaatctgac gttccgcagg gtgctgctat cctgggcgcg atctctggtg
    11641 cgcatcacgt tcaccagatg gctgaacatt atggtgttcc ggttatcctg cacactgacc
    11701 actgcgcgaa gaaactgctg ccgtggatcg acggtctgtt ggacgcgggt gaaaaacact
    11761 tcgcagctac cggtaagccg ctgttctctt ctcacatgat cgacctgtct gaagaatctc
    11821 tgcaagagaa catcgaaatc tgctctaaat acctggagcg tatgtccaaa atcggcatga
    11881 ctctggaaat cgaactgggt tgcaccggtg gtgaagaaga cggcgtggac aacagccaca
    11941 tggacgcttc tgcactgtac acccagccgg aagacgttga ttacgcatac accgaactga
    12001 gcaaaatcag cccgcgtttc accatcgctg cgtccttcgg taacgtacac ggtgtttaca
    12061 agccgggtaa cgtggttcta actccgacca tcctgcgtga ttctcaggaa tatgtttcta
    12121 agaaacacaa cctgccgcac aacagcctga acttcgtatt ccacggtggt tccggttcta
    12181 ctgctcagga aatcaaagac tccgtaagct acggcgtagt gaaaatgaac atcgataccg
    12241 atacccaatg ggcaacctgg gaaggcgttc tgaactacta caaagcgaac gaagcttatc
    12301 tgcagggtca gctgggtaac ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc
    12361 cgcgcgtatg gctgcgtgcc ggtcagactt cgatgatcgc tcgtctggaa aaagcattcc
    12421 aggaactgaa cgcgatcgac gttctgtaag atattccttt ctgcttatct caaggcccgc
    12481 tctgcgggtc tttttttcgc caaaagcaaa attgcctgat gcgctacgct tatcaggcct
    12541 acacgtatcc tgcaatatat tgaatttgca ggtttttgta ggccggataa ggcgttcacg
    12601 ccgcatccgg catgacaaag cgcacattga tatctgtctg agccccgctc tgcggggatt
    12661 ttttcgccaa aagcaaggtg attcagatga gaaatctgtg atctatttgg caaaattatg
    12721 ctttattgtt tacccttgtc agactgcccg tcataaggcg gcggagtgta tttctccatt
    12781 ttgagtcagt tgaaaaggaa tattgaatgg aagatttgaa tgttgtcgat agcataaacg
    12841 gcgcgggaag ctggctggta gctaaccagg cgctgctgct aagttatgca gtaaacatcg
    12901 tggcggcact cgcgatcatc atcgttggtt tgattatcgc gcggatgatt tccaacgcgg
    12961 tgaatcgcct gatgatctcc cgtaaaatcg atgccactgt tgctgatttt ctttctgcat
    13021 tagtccgtta cggtattatc gcctttacgc taatcgctgc cctgggacgc gtgggtgtac
    13081 aaacagcgtc agtcattgct gtactcggtg ccgcaggctt agctgttggt ctggctttgc
    13141 aggggtcact ttctaacctg gccgctggcg tgttacttgt catgttccgc ccgttccgtg
    13201 ccggagaata tgttgacctc ggcggcgtag ccggtactgt gctgagtgtg cagattttct
    13261 ccaccaccat gcgtactgca gacggtaaaa ttatcgttat tccgaacggt aaaattattg
    13321 ccggaaatat tattaacttc tcccgcgagc cagttcgccg taacgaattt attattggcg
    13381 tggcgtatga ttccgatatc gatcaggtta agcagatcct gaccaatatt atccagtctg
    13441 aagatcgcat tttgaaagat cgcgaaatga ctgtgcgcct gaacgaactt ggtgcatcgt
    13501 cgattaattt cgtggtccgc gtctggagca acagcggcga tctgcaaaac gtgtactggg
    13561 atgtgctgga gcgtattaaa cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc
    13621 aaatggatgt gaactttaag cgggtgaaag aagacaaagc tgcgtaatca acgctgaggc
    13681 cagataatac tctctggcct ctctcttatt agttattctg attgccaatt aatattatca
    13741 atttccgcta ataacaatcc cgcgatatag tctctgcatc agatacttaa ttcggaatat
    13801 ccaacgtgtt ttcttattac tttcaaggtc ttgctcttgg ggcagctatg attctgccgc
    13861 tcggcccaca aaatgctttt gtgatgaatc agggcattcg tcgtcagtac cacattatga
    13921 ttgccttact ttgcgctatc agcgatttgg tcctgatttg cgcggggatt tttggtggta
    13981 gcgcgttatt gatgcagtcg ccgtggttgc tggcgctggt cacctggggc ggcgtagtct
    14041 tcttgctctg gtatggtttt ggcgctttta aaacagcgat gagtagtaat attgagttag
    14101 ctagtgccga agtcctgaag caaggcagat ggaaaattat cgccaccatg ttggcagtga
    14161 cctggctgaa tccgcatgtt tacctggata cttttgttgt actgggcagc cttggcgggc
    14221 agcttgacgt cgaaccaaaa cgttggtttg cgctcgggac aattagcgcc tctttcctgt
    14281 ggttctttgg tctggctatt ctcgcagcct ggctggcacc gcgcctgcgc acggcaaaat
    14341 cacagcgcat tatcaatctg gtagtgggat gtgtcatgtg gtttatagcc ttgcagctgg
    14401 cgagagacgg tattgctcat gcacaagcct tgttcagtta ggcgctatct gatggaaaaa
    14461 taaaacagag gcgctaagct tgcctccaga ggtcctgagt tttgggcaag tcaattaaca
    14521 cggagagact aacgtgaagt tcaaagttat cgccctggcg gcattaatgg gtattagcgg
    14581 gatggcagcg caggctaacg aattgccgga tggaccgcat attgtcacct ccggtacggc
    14641 aagtgtggat gcggtgtcag acattgccac tcttgcgatt gaagttaacg tggccgcgaa
    14701 ggatgccgct acagccaaga aacaggcaga tgagcgcgtc gcacaataca tttccttcct
    14761 tgaactcaat cagatcgcga aaaaagatat cagctcagcg aatttacgca cccagccaga
    14821 ttatgattat caggatggta aaagtatcct gaaaggctac cgcgctgtga gaacggtgga
    14881 agtcacgctc cgtcagttag acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg
    14941 tcttaacgaa attcgttctg tgtcgctggg cgtggcgcag cctgatgcct ataaagacaa
    15001 agcgcgtaag gcagcgattg ataacgcgat tcatcaggcg caggaactgg cgaacggctt
    15061 tcatcgtaaa ctggggccgg tatatagcgt gcgctaccat gtttccaact atcagcccag
    15121 cccaatggtg cggatgatga aagccgatgc cgcgccggtg tctgcccagg aaacttacga
    15181 gcaggccgct attcagtttg atgatcaggt cgatgtggtc ttccagttag aacctgtgga
    15241 tcaacaaccc gctaaaacac ctgcagcaca ataatacttg tagcccgacg tattcgtcgg
    15301 gctaatgcct ttacccgata ttgcctcctt tttctgtaat tttctgtaaa accatgacgt
    15361 aatcacctat ctttgctcgc ggtttcatca aggataggct gactgaaacg gttaagcgga
    15421 caaggatgcg gggagggggg atggacattt ttatttcgaa aaagatgcgc aatttcattt
    15481 tattagcgca aaccaataat attgcccggg cggcagaaaa aatccatatg tcggcttcgc
    15541 catttggtaa aagcattgcc gcgctggaag aacaaattgg ctatacgcta tttacccgca
    15601 aagataataa tatcagcctc aataaagccg gtcaggaact gtatcaaaaa ctgttcccgg
    15661 tttatcaacg actttctgcg attgataatg aaatccataa ctcagggcgt cgttcacgag
    15721 aaattgtgat tggcatagat aatacttatc cgacaattat tttcgatcag ttgattagtc
    15781 tcggagataa gtatgaaggc gtgaccgccc agccagttga gttcagcgaa aacggcgtta
    15841 tcgataatct ttttgaccgt cagttagatt ttattatctc cccacagcat gtatctgcac
    15901 gagttcagga actggaaaac ctgactatca gtgaattacc accgctgcga ttgggctttc
    15961 tcgtttctcg ccgttatgaa gagcgtcaag agcaagagct gcttcaggag ctgccctggc
    16021 tacaaatgcg ctttcaaaac cgggctaact tcgaagcaat gattgatgcg aatatgcgac
    16081 cttgtgggat caatcccacc attatttatc gtccctacag ttttatggcc aaaatcagcg
    16141 ccgtggagcg cggacatttt ctgacggtga tcccgcattt tgcctggcga ctggtgaatc
    16201 cggcaacgct gaaatatttt gatgcgccgc acaggccgat gtatatgcag gaatatcttt
    16261 attcaatcag aaatcatcgt tataccgcca cgatgcttca gcatattgct gaagatcgtg
    16321 acgggacaaa ccattaaccc agcatcgagc cggttgcaat taaattacgg tgtaagtcga
    16381 agacgtggct aagatcgtgg tgaatatgtc cgccgggcgc attttccaga tagcgatgca
    16441 gataatcccg atacatagga tgtgcacaat tatcaatgat agtgcgggcg cgttgaagcg
    16501 gggaaagacc gcgcagatcg gcgatccctt gttcggtgat gatcactttg acgctgtgtt
    16561 cgctgtgatc aacatggctg cacattggca cgacggttga gatcttgcct tctttagcaa
    16621 tcgacggagc cataaagatc gacagatacg cgttgcgttc aaaatcaccg ctgccgccga
    16681 tgccgttcat cagatcgacc ccggctacgt gcgttgagtt ggcatgcccg taaatatcaa
    16741 actccaggcc gacgttcaga gcgatgacgc ccagacgacg gatgatttcc gggttattgg
    16801 aaatctcctg cggacgcaac acaatgcggc tggcaaagta atccatattg tcgtaaatct
    16861 tgcgcaggga atcggccgag attgtcaggc tggaggcgct ggccccgctg attttgccgg
    16921 tttccagtaa atgcaccacc gattcctgta gcacttccga atacatcata aacggaggaa
    16981 tttccgggtt ttcccccaga cgcgccatta ccgcattatt gatattgccc acgccacttt
    17041 gcagcggcag aaattccggc ggaatacgcc catgcgccat ttcctgcaat aagaacgtga
    17101 ccacgttatc ggcaatctgc tggcacatgg gattttgctt atccagcata ttaccggcgt
    17161 cgggcaagtt ggtttccacg acggcgacaa tctttttcgg atcgatttgc acatagcggg
    17221 taccgacgcg atccattgca tggaagatcg acacgctatt gcgccgcggt ggcgcgccag
    17281 gaatcacaat atccgccagt tctgcaacgc gcggatcgtg atagtgatta agttcaatga
    17341 tcactttctt cgcccgcagc agccatgtcg gcgcattacc gatcccgctg gttaaccaga
    17401 ctcgaccatc cggtgccagt gccgatgctt cgatgacggc aacatcaatg tcgccgaaga
    17461 aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag gtcaacgaaa ctcaccgcgc
    17521 cctgattgat ctttttacgt aaaccggacg atgtttgata tggcgcacgc caggaaacag
    17581 catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat tgacgccccc gtcagcaggc
    17641 gaatttgata cggctttttg gcctcatgct gttcgttagc tctgcgggca atcgcggtcg
    17701 gtaaggcttt cggcgaaccc gctggggtaa agccgctaaa tgccaccatg tcgttatgct
    17761 ggataatttc tgccgcttca tcggcggtca tccttgtcca ctgagtttcc atttttactc
    17821 cttcgttcgc agggattaat gaccaacgaa attaggttta cgtttttcga ggaaagcgtt
    17881 catcccttcc tggtaatctt cgctgtcata aaccgcgcgg cgcatcccct gaatacgttc
    17941 aaattcatcg gagttcatgg tgtgcgcttc gcccagtaca cgcagctctt ctttgataac
    18001 agcaatggct aacggcgctt tctcagagat gtggtgcgcc atttgtaagg tgaaatcttc
    18061 cagttcttcc acttccacaa catggttgag gatgccgaca gccaacgcgc gctgggcggt
    18121 gattggcgaa gcggtaaaaa tcagttcttt gacaatgtgg aagcccgcgt cgcgggtcag
    18181 gttgtgaatg ccgaccaggt tatacgggac gccgaggttt acaggcgtca ttgagaaggt
    18241 tgaggtactg gcggcgataa tcagatcgga actcatgatc atttcaaatg cgccacccca
    18301 aacactacct tccaccatcg aaatgatcgg tttcgggaat ttttggatca tgcgggtgat
    18361 ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg ccagacggca gttcgtgaat
    18421 agcgtggcct gcggagaaga ctttggatcc actcggcgcg cgcaaaatga tacagcgaat
    18481 ttccggccgg ttgagatcgc ttaatgcctg cataagatca tcaataaaga ctttacttaa
    18541 ggcattaagt ttgcggccat agttaaactc aatgaccgcc actttgttga tagtgacaac
    18601 gttaacatac tgataagaca taaaaattcc tttaatcaaa atattgcgtc tggataaatt
    18661 cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc gctaaaagcg
    18721 tctggcggta atagcgatcg aaatcttcat tcgcgaacag gtgattcagt acttcttctt
    18781 cggtctgctt acgcagccat tccaccgatt gttgttgccg cacttgttgt aaacgaccac
    18841 tggcagttag cgcggttttg aagtcgatga tggcgtgcca gatctcatcg attccacgtt
    18901 tttccagtgc gctacaagtc agaacccgtg gctgccattc gtcgtattta cgtcgcagaa
    18961 tatgcagggc actctcgtac atatgccggg caatggcgac attggtatgg ttatcgccat
    19021 cgtctttgtt gataacgatc agatcagcca cttccatcag ccctttttta atgccctgca
    19081 gatcatcgcc gccaccggca atttgcaacg agataaaaca gtccaccatg cgggcgactt
    19141 ctgtttccga ctgcccgacg ccaaccgttt cgacaatcac tacgtcataa cccgctgctt
    19201 cgcataacag cattaattcc cgcgctcgct gactggcacc gcccagatga ccggaggatg
    19261 gtaccgggcg aataaacgcc gcttcggcac gcgccaggtc attcatgcgg gttttatccc
    19321 cgagaatgct accgccagtg accgggctgc tgggatcgac cgcaataacc gcgaccttta
    19381 atccctctcg aatcaacaac atgccaaagg cctcaagaaa ggtacttttc cccgcgccgg
    19441 gggtgccggt aacgcccagt cgcagggtgt taccgcagta cggcataatg gcatcaagca
    19501 gctgcgtact tagtgcctga tgacgcgggt gacggctttc caccagcgtc atggcctggg
    19561 cgagtgtggc acgctcaccc tgacgtaagc ggcgaatact ttctgccagc gtggcttcat
    19621 taatcatgat gctggcttat cagattcagt acgtcgcgca cactgtcgag cataggtgta
    19681 cctggaccat aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc ctgcggcgga
    19741 atgacgccac ccgcgaccac gcagatatct tcgcgtcccc attttttcag cgcttcgacc
    19801 agttccggga tcagcgtttt atgaccggca gccagtgagg atgcgcccac tacgtgaacg
    19861 tcgttttcta cggccaggcg ggcgatctct tcaggtgtag agaacatcgg gcttaaatct
    19921 acgtcgaaac cgagatcgga ataggcgctg gcgatcactt tcgcgccgcg atcgtgtcca
    19981 tcctggccca tcttagcgat cagaatgcgc gggcgacgac cattgtcggc aaggaactgc
    20041 tccgtttgcg caacaatggc atcgaactcg gaggccgatt tctcagactg atgatagctt
    20101 tgcgcaatca cgccggtaac acactggctt ggcaccagat aacggtcgaa agcgacttcc
    20161 agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag cggcattaac agcggcagcc
    20221 agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc ggttacggcg
    20281 gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa tttgctcgtt acgcaccatc
    20341 acgttgtcga tctcaagtac atcggtttcg tcttcgtgat ccagtttgta cttgttgaca
    20401 ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt cgcgcgctga ggcctcttcg
    20461 atcattcgtt ttggcagacc tgcttcgatc gctttcgcca tgccaccggc ttcgtcgatc
    20521 tgttggataa tagctctggc ttgtttgacg atttgatcgg tcagcgactc aatgtaatag
    20581 gatccggcca gtggatcgac gatgcggcag agttctgatt cttcctggat gatgatctgg
    20641 gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc gtcaaaggcg
    20701 ttggtatgca gtgactgagt accgcccagc gtcgcagcca gcgcttcaat ggtggtgcgg
    20761 ataacgttgt tatacggatc ctgttcagtc aggctccagc ctgaggtctg gcagtgggta
    20821 cgcagcgcca gtgatttcgg gtcctgtgcg ccaaatccac tgaccgcttc gctccataaa
    20881 taacgtgccg cacgcaacat ggcgacgttc ataaacagat ccatgccgat gccgaagaag
    20941 aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg cagagattgc tgctttgatg
    21001 tactcaatcc catcagcgag cgtaaatgct acctgctgca cgcagttggc acccgcttca
    21061 cccatgtggt aaccgctgat actgatggta ttaaatcgcg gcatgttgcc ggaacaccag
    21121 gcgatgatgt cggcgataat gcgcattgac ggttttggcg ggtaaatata ggtgttgcgg
    21181 cagaggtact ctttgaggat atcgttctga atggtgccgg tcagtttatc aggtgtaaca
    21241 ccttgctctt ccgcggcgat gatataaaac gccagtactg gtagcactgc gccattcatg
    21301 gtcatcgaaa ccgacatttt atccagcggg atctggtcga acaggacttt catatcttcc
    21361 acggtgtcga tagcgacgcc cgctttgccg acgtcacccg ccacgcgtgg gttatcggag
    21421 tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg cccggcggcc
    21481 aggttacggc gataaaaagc gttggactct tttgctgttg aaaaaccagc atactgacgg
    21541 atggtccacg gttgggcggt atacatagtg gcacgcgggc cacgaacgta gggcggcaaa
    21601 ccaggaaggg tacctgtcac ctccagatta tcgagatcgg cttcggtata cagcggcttg
    21661 atggcgatcc cttccgcggt ttgctgaacc agcgagtcga cagttttctc ccgacggctc
    21721 aattccttgt tggcaagctg ttgccactcc tgctcgttag acattatttg ctccgtaaaa
    21781 cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt ttacgtgggt ttgtcgccag
    21841 caaaaaaaat gcattcagac gtcgtttttt gccgcctgag atttgtctat tttgtgatgt
    21901 gattcaagga atacaaaaag aagtgaggat aacgcctgat atgtgctatc aggcattatt
    21961 tgatggatta atcctgacga aggactttgt gaccataatc gagcaacgca tcagtgactt
    22021 tacgcatcat gcggctttca ggagcaaagc ggtgccagta gagcatccgg cgttgaaata
    22081 gccctggcgt taagtcaatc agttcaccgc tggctagttc tttctcgatt tgcaggtgcg
    22141 ggatcataca gcaggtggtg ccctggcgag caagttgcac gaacgcttct gaagaattaa
    22201 cgatatggca gggcacgctg cctggaggca gatcgaagtt ttgctgcaaa aaggcctggt
    22261 gcatatcgtc aagatggtca aaggcgacca caggtgcttt caataatgcc gaacgcgtta
    22321 cgccgttagg gaaatatttt tcggcaaagg gttttgagct gacgaacaga tagtcgagcg
    22381 caccaagttt atcgacaaga caactcggca gcgcctgatg ttgaatactc accgcgccga
    22441 ccacttcgcc gcggcgcaga cgttcctgag tgcgggtttc atcttctacc tgcaagttga
    22501 ggcggatagg cgaatcagcc aacacaggag ccagtgcagg aagcaaccac gtcgccagac
    22561 tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga accggtttgt tcatcgccca
    22621 gccactcttc ttccagcaac tccacctggc gcagcagtgc cagcagtttt tgcccttgtt
    22681 ccgtcgggcg cggcggtacg gtacgcacca acagcggctg cccgaacata ttttccagtt
    22741 gcttaatgcg ctgtgagacg gctgattgtg taatgcacag cttttgtgcc gcgcgctcaa
    22801 atcctcgttc acgtatcacc gcatccagtg cctgtaatgt tctgtagtcc gggcgtttca
    22861 ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact atgacacaat tttgtgtcag
    22921 gttgcatata ccgaagccgt cggcgggtaa catttgtatt cgtggttaaa cgcgcagctt
    22981 aaaagatcag ggatttgcgc taatgcattt cgctcagcga gttcgcgccc tggttgtgtt
    23041 gaatggcgtg gcgttattgc ctctgttcgc accgtggagc ggcataccca gaccgttgta
    23101 tgctttattt gcggggcgaa aggggatgcc cgccattgcg cgatatttta tggatgagtt
    23161 aaccacgcgg cttgccaacg gggtctgaat cgcttttttt gtatataatg cgtgtgaaat
    23221 ttcataccac aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca gtaggatggg
    23281 cggcacttca gtatgttcag cccggcacca ttgttggtgt aggtacaggt tccaccgccg
    23341 cacactttat tgacgcgctc ggtacaatga aaggccagat tgaaggggcc gtttccagtt
    23401 cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat ctcaacgaag
    23461 tcgacagcct tggcatctac gttgatggcg cagatgaaat caacggccac atgcaaatga
    23521 tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt gcagaaaaat
    23581 ttatctgtat tgcagacgct tccaagcagg ttgatattct gggtaaattc ccgctgccag
    23641 tagaagttat cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa ctgggcggtc
    23701 gtccggaata ccgtcagggc gtggtgaccg ataatggcaa cgtgatcctc gacgtccacg
    23761 gcatggaaat ccttgacccg atagcgatgg aaaacgccat aaatgcgatt cctggtgtgg
    23821 tgactgttgg cttgtttgct aaccgtggcg cggacgttgc gctgattggc acacctgacg
    23881 gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa aaaattctct
    23941 tcattaaatt tggtgacatg tgtcacgttt ttacctggct attgtcgatt gctctaaata
    24001 aatcttctaa accagcatat tcatccaaga attacctttg cgtgatattt cctcaacatc
    24061 gcgacgcaaa cgttcatatt gccgcaatat tattttttga tatgttgaaa ggcggacgca
    24121 aatccgcaca caacatttca aaagacagga ttgggtaaat ggcaaaggta tcgctggaga
    24181 aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg gaaagccttc
    24241 gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat gaacaattaa
    24301 aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat ctgactgaag
    24361 acgtgatcaa cgccgcagaa aaactggtcg ctattggctg tttctgtatc ggaacaaacc
    24421 aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca ccgttctcaa
    24481 atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg cgcggcgtgc
    24541 cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg ggttcttttg
    24601 aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg caattgggca
    24661 ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaat aaactgccgc
    24721 tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc gatgtggtga
    24781 gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa gaaatttcac
    24841 taatgaagcc cggctcgctg ctgattaatg cttcgcgcgg tactgtggtg gatattccgg
    24901 cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac gtattcccga
    24961 cggaacctgc gaccaatagc gatccattta cctctccgct gtgtgaattc gacaacgtcc
    25021 ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc ggcctggaag
    25081 ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg gtgaacttcc
    25141 cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac gaaaaccgtc
    25201 cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac atcgccgcgc
    25261 aatatttaca aacttccgcc cagatgggtt atgtagttat tgatattgaa gctgacgaag
    25321 acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt cgcgcccgtc
    25381 tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt gcccgttttt
    25441 ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc tgctccagaa
    25501 aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac gatggcggga
    25561 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg agaagggtta
    25621 tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga gcttattaac
    25681 agtcagtctc aggggaggag tcatcctccc ttacccttac tcactaaatt aggtcaaaga
    25741 atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac tttttgatag
    25801 ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa ttatctctgt
    25861 tttgcatggg gtttttcgag cacgctcgaa gaaaagattt aaaggtagat caatgcgttg
    25921 tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg gtaaagccct
    25981 caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca tactgattgt
    26041 actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg agcaatcctc
    26101 ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag ggcgatgcag
    26161 gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg aagagggata
    26221 tcccactctt caacggggag tttttccacc aactgacaat catgcgcata acccaccggt
    26281 tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc gcccattccc
    26341 aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc taatcgggaa
    26401 aggggtagca cgtcacgcac atccagtttt ggctcatgga tcttcaacct gttcatcacc
    26461 agttcgcttt gcggatggta attcaggaac agcaaattac cggcactaaa gggatgcaaa
    26521 actggaagat atacgcgctt accggcgcgc cagagttgtt ctattagcgg ctgggtgtcg
    26581 agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac cgggggataa
    26641 gtcatcatcc gggtagcggc ttgttgaccc atttcctgct gttgttccgg cgttaacgca
    26701 cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag tgggagttct
    26761 ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc cgccgcaggc
    26821 tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa ggcttctcgg
    26881 acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg aaatatcggc
    26941 tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg gttactctac
    27001 cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg atgcgacctt
    27061 gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt tccatacttg
    27121 ccgcgtagtc acgagtcttc gctttttctt gcgctaactc atagctgata ttcaatgcgg
    27181 caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga tcttgcaacc
    27241 gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc gggcagttca
    27301 cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg ccaccttcct
    27361 gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg cgacactata
    27421 gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa agtggtagca
    27481 tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa cgaaatgcct
    27541 ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac ccctgctgag
    27601 atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc atggctaccg
    27661 ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc acaggcactg
    27721 cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct ttttcagctt
    27781 tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt ggcaggttgg
    27841 gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggataa agtgaccggc
    27901 gaaaccggtg aagctatcga cgatctgcgt aacattgcgc aactgggtta cgacgaagac
    27961 gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt tcgtgttgcc
    28021 gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga agtacaaaaa
    28081 ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga tatcccggca
    28141 agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca gcgccgcgct
    28201 gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct atcgtcagaa
    28261 cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg tgctgattaa
    28321 aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc tgacggcgga
    28381 gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg gcgttgaccg
    28441 cgcactggca ttcagcgaaa tcaatcagca actttatcaa ctacttaacg gcctggatgt
    28501 ggtttaccat gcccagggcg aatataccta tgctgatgaa atcgtgaaca gtgcgctgga
    28561 aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga tcgactggcg
    28621 tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg tactccgccg
    28681 cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc gtccgggaat
    28741 gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccacg gtgcgcgcta
    28801 tccgtcctat aacaccattg tcggcagcgg tgaaaacggc tgcattctgc actacaccga
    28861 aaacgagagt gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt gtgaatacaa
    28921 aggttacgcg ggcgatatta cccgcacctt cccggtcaac ggcaaattca cccaggccca
    28981 gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcctgcgcc tgtatcgtcc
    29041 gggaacttcc attctggaag tcactggtga agtggtgcgc atcatggtta gcggcctggt
    29101 aaaactcggc atcctgaaag gtgatgttga tgaactgatc gctcagaacg cccatcgtcc
    29161 tttctttatg catggcctta gccactggtt aggactggat gtccatgacg ttggcgttta
    29221 tggtcaggat cgctcgcgca ttctggaacc ggggatggta ctgaccgtag agcctggtct
    29281 gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc ggtatcggca ttcgaattga
    29341 agacgacatt gtgattaccg aaaccggtaa cgaaaacctc accgccagcg tggtgaaaaa
    29401 gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt aatcatcgtc
    29461 ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag tcacggggcg
    29521 ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc gggctttgat
    29581 ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat cggcgtctgg
    29641 caatctctgg cggattgcgc aactgccatc accaccgtgc atgtcagcga tcgtggtcac
    29701 gctggatttg tcaccctcgc cgcagaagat taccaactgg cggcgctggg acaggttgtc
    29761 gaattgcaca atgtcgggca acggctgttt gcattgctgc gtaaagcacc tggcgtaatg
    29821 ctgcattgcc ccgatcgcgt agctaacgtt gcccgtactc agagtcacgt tgaagtgacg
    29881 ctggagagtg gcgagacgct gacgggccgc gtgctgatag ccgctgatgg cacccattca
    29941 gcgttagcca ccgcgtgcgg cgttgactgg cagcaggagc cttacgaaca actggccgtg
    30001 attgccaacg ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg cttcacgcaa
    30061 catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt ctggtgtcat
    30121 ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg ccgtgaactc
    30181 cagtcggcct ttggctggcg acttggaaaa attacccacg ccggtaaacg cagtgcttat
    30241 ccgctggcgt taacccgcgc cgccagagct attacccatc gtaccgtgct ggtgggcaat
    30301 gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat gcgagatgtc
    30361 atgagccttg cggaaaccct gactcaggcg catgagcgcg gagaagacat cggtgattac
    30421 ggcatattgt gccgctatca gcagcgtcga cagagcgatc gcgaagcaac catcggcgtc
    30481 acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt cgggcgcaac
    30541 atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc gcagcgcacc
    30601 ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaaa gtgttgatgt
    30661 agccattgtt ggtggcggca tggtggggct ggcggttgcc tgcggcttac aggggagcgg
    30721 cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggtggcgg atgcaccacc
    30781 acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc gtcttggcgt
    30841 ctggcaggac attctctctc gtagggccag ttgttatcac ggtatggaag tgtgggacaa
    30901 agacagtttt ggtcacattt cgtttgacga tcaaagcatg ggctatagcc atcttgggca
    30961 tatcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc agtcgtcaga
    31021 tatcactctg ttggcccccg cagaattaca gcaggtcgcc tggggagaaa atgaaacttt
    31081 cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg cggacggtgc
    31141 taattcctgg ttgcgcaaca aagcagatat tccgctgact ttctgggatt atcagcatca
    31201 cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc ggcaggtttt
    31261 ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt gctcaattgt
    31321 ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag atgaatttaa
    31381 tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgcaaggttg agagcgcgcg
    31441 tcaggtgttc ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc gtctggtgct
    31501 ggtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggagtaa atctcggctt
    31561 tatggatgct gcagagctga ttgctgaact gaaacggttg catcgtcagg ggaaagacat
    31621 cgggcagtac atttacctgc gccggtatga gcgtagccgc aagcacagtg cggcgctgat
    31681 gctggctggt atgcagggat tccgcgatct gttttccggt accaatccgg cgaaaaaact
    31741 gctgcgtgat attggtttga aactggccga cacgcttcct ggcgttaaac cacaacttat
    31801 ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat ttctcttctg
    31861 ctgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg agattttcct
    31921 catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat gtcattattt
    31981 tttgatttag tgttttttga cattttttta gctcttaata ttgccttatt caaattagct
    32041 ttctaatggc atcatcttta tatagaaact ggtgtgtttt ttggtttttt attctgtcgc
    32101 gattttggcg ttttttaacc ataagctaat gtgatgatca attttacctt atggttaaca
    32161 gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac cgggagtggc
    32221 tccgatgctg ggtttcgtgg tgataatttc accatgaaaa gttgtcagcc ccgcttattc
    32281 aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct ttgcggcgct
    32341 cgcatggtgg atttccacgg ctggatgatg ccgctacatt acggttcgca aatcgacgaa
    32401 catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac catcgtcgat
    32461 ctccgcggca gccgcacccg ggagtttctg cgttatctgc tggcgaacga tgtggcgaag
    32521 ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg cggtgtgata
    32581 gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt taactccgcc
    32641 acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg catcgaaatt
    32701 accgttcgtg atgacctttc catgattgcc gtgcaagggc cgaatgcgca ggcaaaagct
    32761 gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc gttctttggc
    32821 gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc gggctatgaa
    32881 attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga agcgggtgtt
    32941 aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat gaatctttat
    33001 ggtcaggaga tggacgaaac tatttctcct ttagccgcca acatgggctg gactatcgcc
    33061 tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc tggaagtgca gcgtgagcat
    33121 ggtacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg taatgaactg
    33181 ccggtacgtt ttaccgatgc gcagggcaac cagcatgaag gcattatcac cagcgggact
    33241 ttctccccga cgctgggtta cagcattgcg ctggcacgcg tgccggaagg tattggcgaa
    33301 acggcgattg tgcaaattcg caaccgtgaa atgccggtta aagtgacgaa acctgttttt
    33361 gtgcgtaacg gtaaagccgt cgcgtgattt acttttttgg agattgattg atgagcaacg
    33421 taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa gccgacggca
    33481 ctttcaccgt tggtataacc gaacatgccc aggagctgtt aggcgatatg gtgtttgttg
    33541 acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt gccgaatcag
    33601 taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg gtaaacgacg
    33661 cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggtggc tggatcttta
    33721 aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc gcatacgaag
    33781 cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat cagggtgagg
    33841 aaaatttatg cctcaccctc actctcttcg taaggagaga ggttcacaat tcactgcacg
    33901 tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag cggcgctttt
    33961 attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa tgccgttggt
    34021 gcacaatcgt taaacgcgct gaccggccag attgtgccga aagatattca gcttgcgacc
    34081 ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact caaggctatt
    34141 gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc cgtgcagcta
    34201 ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc gtacactccg
    34261 tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca gcaggtaacg
    34321 ctggatttga ctggactgga tatggcctct gcttctcttc tggacgaggc caccgctgcc
    34381 gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttc
    34441 gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc cgaaaccttt
    34501 ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga cgtcttcggc
    34561 gtgctgttac agcaggttgg cacaaccggt gaaatccacg actacaccgc gcttattagc
    34621 gaactgaaat cacgcaaaat tgtggtcagt gtcgccgccg atattatggc gctggtgctg
    34681 ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca acgcttcggc
    34741 gtgccgatgg gctacggtgg cccacacgcg gccttctttg cggcgaaaga tgaatacaaa
    34801 cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa taccgcgctg
    34861 cgcatggcga tgcagactcg cgagcaacat attcgccgtg agaaagcgaa ctccaacatt
    34921 tgtacttccc aggtgctgct ggcaaacatc gccagcctgt atgccgttta tcacggcccg
    34981 attggcctga aacgtatcgc taaccgcatt caccgtctga ccgatatcct ggcggcgggc
    35041 ctgcaacaaa aaggtctgaa actgcgccat gcgcactatt tcgacacctt gtgtgtggaa
    35101 gtggccgaca aagcgggcgt actgacgcgt gccgaagcgg ctgaaatcaa cctgcgtagc
    35161 gatattctga acgcggttgg gatcaccctt gatgaaacaa ccacgcgtga aaacgtaatg
    35221 cagcttttca acgtgctgct gggcgataac cacggcctgg acatcgacac gctggacaaa
    35281 gacgtggctc acgacagccg ctctatccag cctgcgatgc tgcgcgacga cgaaatcctc
    35341 acccatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta tatgcactcg
    35401 ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg
    35461 aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc cgaactgcac
    35521 ccgttttgtc cgccggaaca ggctgaaggt tatcagcaga tgattgcgca gctggctgac
    35581 tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc cggcgcacag
    35641 ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa cgaagggcat
    35701 cgcgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc tgcacatatg
    35761 gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga tctgactgat
    35821 ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt gacttatcct
    35881 tctacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc
    35941 ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat cacctcgccg
    36001 ggctttattg gcgcggacgt ttcgcacctc aacctgcata aaactttctg cattccgcac
    36061 ggcggtggtg gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc accgtttgta
    36121 ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc agtttctgcg
    36181 gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg catgatgggc
    36241 gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta tattgccagc
    36301 cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt ggcgcacgaa
    36361 tgtattctcg atattcgccc gctgaaagaa gaaaccggca tcagcgagct ggatattgcc
    36421 aagcgcctga tcgactacgg tttccacgcg ccgaccatgt cgttcccggt ggcgggtacg
    36481 ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt tatcgacgcg
    36541 atgctggcta tccgcgcaga aatcgaccag gttaaagccg gtgtctggcc gctggaagat
    36601 aacccgctgg tgaacgcgcc gcacattcag aacgaactgg tcgccgagtg ggcgcatccg
    36661 tacagccgtg aggttgcagt attcccggca ggtgtggcag acaaatactg gccgacggtg
    36721 aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt accgattagc
    36781 gaataccagt aattcactga ttcgactatt ttctaaaggc gcttcggcgc ctttttagtc
    36841 agatgacaaa gtacaaaagt gctcagacag tcccctcgcc cctttgggga gagggtgagg
    36901 ggaacaggcc ggcactggcg cgaatattta ccctcacccc ggccctctcc ctgaaagggc
    36961 gagggggaaa agcgtgccaa cattgaagat tgagccagtt tgttagcaat ctcaaagata
    37021 cgtcaacgaa tgaatttttc tcggaaaaac aaatggttat agcacttgtg accggtggca
    37081 gtcgcggcat cgggcgggca actgcattac tgttggcgca agaagggtat acggtggcgg
    37141 ttaattatca gcaaaacctc cacgcggcgc aggaagtgat gaacttaata acgcaagccg
    37201 gtggcaaagc attcgtgctc caggcggata tcagcgacga aaaccaggtc gttgcgatgt
    37261 ttacagcaat cgatcagcac gatgaaccgc tagcagcgct ggtcaataac gccgggatct
    37321 tgtttaccca gtgcaccgtt gaaaacctta ccgcagagcg aatcaaccga gtactttcca
    37381 ccaacgtgac gggatatttt ctctgctgcc gcgaggcggt aaaacgcatg gcgcttaaaa
    37441 atggtggcag tggcggcgct atcgtcaatg tctcttcggt ggcctcacgg ttgggttcgc
    37501 caggggaata tgttgattac gcggcatcga aaggggcgat tgatacgtta accaccggac
    37561 tatcgctgga agtcgccgcg caggggatcc gcgttaactg cgtgcggcca gggtttattt
    37621 ataccgaaat gcacgccagc ggcggcgagc ctggacgcgt cgatcgcgtt aagtcgaaca
    37681 tccccatgca gcgtggtgga caggcagaag aggtcgcgca ggccattgtc tggctactaa
    37741 gtgataaagc ctcttacgtc acgggaagtt ttatcgattt ggcgggcggg aaataaaaca
    37801 gggaagttgt ctgaccggat gcaacaagta ttgcatccgg tacttcatcg acttaaagct
    37861 tctcgccgtt gctggcaatc acctcttggt accagttaaa gctcttctta cgtgaacgcg
    37921 acatatcgcc agcaccgtcg tcatgtttat tcacatagat aaagccgtag cgtttgctgt
    37981 actgcccggt ggtgaacgat acgcagtcga tgcagcccca cggtgtgtag cccatcagat
    38041 ccacgccatc gtaagtcacc gcttttttca tctcttcgat atgggcgcgc aggtagtcaa
    38101 tgcggtagtc gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt
    38161 tttcgacaat aaacagcggc ctctgataac gctcatacag ttcgcaaagg gcatagcgca
    38221 gacctactgg atcaatctgc cagccccagt cagacgcttt aacatacggg ttcggtacgc
    38281 tgccttcgaa accagagatc gcatcgccgg tgccgccttc ggccttcact gcgttggtca
    38341 tgtaatagct gaaaccaaga taatcgcagg tgccttcacg cagtacatcg agatcgccgt
    38401 cttccatttt gatgttaaat ccgcgacgct cccactcgtt caacacatag gacgggtaat
    38461 agccgcgtag ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca
    38521 tcacatcgtc cgggttacag cagtagggat agagcggcac catcgccagc atacagccga
    38581 ctttcatctc cgggttaatg cgacgcgcag ctttcaccgc cagggcgctg gcgacaaact
    38641 ggtgatgcag cacctggtac atcgtctctt ccgggttgtc atgctcggta taaaccacgc
    38701 cggagcagca gtaaccgaac agcggtgcac gccagttacg ctggttgtta atttcgttga
    38761 aggtcatcca gtatttgact ttatgcttat agcgttcaaa caccacttcc gcgaaacgga
    38821 caaagaaatc aaccacttta cggttggtcc agctaccgta ttgctgcacc agatgcagcg
    38881 gcatttcaaa gtgggagagg gtgatcaccg gttcgatgtt gtatttcagc agttcatcga
    38941 acatatcatc gtagaacttc agcccttctt cgtttggctg agcttcatca ccttttggaa
    39001 aaatacgcgt ccaggcgatg gaagtccgaa aacatttgaa gcccatttcg gcaaatagct
    39061 tgatgtcttc cttgtagtga ccataaaaat caacggcttc atggtttgga tagtatttgc
    39121 ctggcaagac ttctttggtg atttcgcgcg gcacgccgtg tgcgccaccg gtcagaacgt
    39181 cacaaatgct cggccctttg ccgcctttgt tccagctgcc ttcgacctga tgagcggcaa
    39241 ctgcgccgcc ccataagaaa tctttcggta aggtgagttt tttcactatc ataggctcca
    39301 gatgagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac tttttttcaa
    39361 cgtttccagg atgacattct ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa
    39421 tcgcacttca aaacgggtga tgtgcttcgt gtcgggcgtt ttgaagatga cggttatttt
    39481 tgcacgattg aagtcaccgc aacctcaacc gtaaggctgg atacgctgac tgaaaaacat
    39541 gcagagcagg aaaatatgac cctgactgaa ctgaaaaagg tcattgccga catctatccc
    39601 ggtcagacac agttttatgt gattgaattt aaatgtcttt aaattgaact cacgcctgtt
    39661 agttgaaatt tctcatttaa t
//
LOCUS       DAZFWP020000025        39341 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFWP020000025 DAZFWP020000000
VERSION     DAZFWP020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.025"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            204..623
                     /gene="umuD"
                     /locus_tag="ACHK0Z_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             204..623
                     /gene="umuD"
                     /locus_tag="ACHK0Z_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4501253.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            623..1891
                     /gene="umuC"
                     /locus_tag="ACHK0Z_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             623..1891
                     /gene="umuC"
                     /locus_tag="ACHK0Z_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU4501254.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1937..2467)
                     /gene="dsbB"
                     /locus_tag="ACHK0Z_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             complement(1937..2467)
                     /gene="dsbB"
                     /locus_tag="ACHK0Z_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU4501255.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2613..4154)
                     /gene="nhaB"
                     /locus_tag="ACHK0Z_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             complement(2613..4154)
                     /gene="nhaB"
                     /locus_tag="ACHK0Z_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU4501256.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4376..5095
                     /gene="fadR"
                     /locus_tag="ACHK0Z_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             4376..5095
                     /gene="fadR"
                     /locus_tag="ACHK0Z_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU4501257.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5147..6679)
                     /gene="ycgB"
                     /locus_tag="ACHK0Z_RS06005"
                     /old_locus_tag="pgaptmp_001201"
     CDS             complement(5147..6679)
                     /gene="ycgB"
                     /locus_tag="ACHK0Z_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU4501258.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7009..8307
                     /gene="dadA"
                     /locus_tag="ACHK0Z_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             7009..8307
                     /gene="dadA"
                     /locus_tag="ACHK0Z_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU4501259.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8317..9365
                     /gene="dadX"
                     /locus_tag="ACHK0Z_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /pseudo
     CDS             8317..9365
                     /gene="dadX"
                     /locus_tag="ACHK0Z_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(9751..11487)
                     /gene="cvrA"
                     /locus_tag="ACHK0Z_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             complement(9751..11487)
                     /gene="cvrA"
                     /locus_tag="ACHK0Z_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU4501260.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11582..12496)
                     /gene="ldcA"
                     /locus_tag="ACHK0Z_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             complement(11582..12496)
                     /gene="ldcA"
                     /locus_tag="ACHK0Z_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU4501261.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12596..13207
                     /gene="emtA"
                     /locus_tag="ACHK0Z_RS06030"
                     /old_locus_tag="pgaptmp_001206"
     CDS             12596..13207
                     /gene="emtA"
                     /locus_tag="ACHK0Z_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU4501262.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13209..13943)
                     /gene="ycgR"
                     /locus_tag="ACHK0Z_RS06035"
                     /old_locus_tag="pgaptmp_001207"
     CDS             complement(13209..13943)
                     /gene="ycgR"
                     /locus_tag="ACHK0Z_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGU4501263.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14144..14398
                     /gene="ymgE"
                     /locus_tag="ACHK0Z_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             14144..14398
                     /gene="ymgE"
                     /locus_tag="ACHK0Z_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4501264.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14576..15016
                     /gene="ycgY"
                     /locus_tag="ACHK0Z_RS06045"
                     /old_locus_tag="pgaptmp_001209"
     CDS             14576..15016
                     /gene="ycgY"
                     /locus_tag="ACHK0Z_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HGU4501265.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15095..16792)
                     /gene="treA"
                     /locus_tag="ACHK0Z_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             complement(15095..16792)
                     /gene="treA"
                     /locus_tag="ACHK0Z_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4501266.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17112..18530)
                     /gene="dhaM"
                     /locus_tag="ACHK0Z_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             complement(17112..18530)
                     /gene="dhaM"
                     /locus_tag="ACHK0Z_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGU4501267.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(18541..19173)
                     /gene="dhaL"
                     /locus_tag="ACHK0Z_RS06060"
                     /old_locus_tag="pgaptmp_001212"
     CDS             complement(18541..19173)
                     /gene="dhaL"
                     /locus_tag="ACHK0Z_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGU4501268.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19184..20254)
                     /gene="dhaK"
                     /locus_tag="ACHK0Z_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     CDS             complement(19184..20254)
                     /gene="dhaK"
                     /locus_tag="ACHK0Z_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGU4501269.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSRVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20482..22401
                     /gene="dhaR"
                     /locus_tag="ACHK0Z_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     CDS             20482..22401
                     /gene="dhaR"
                     /locus_tag="ACHK0Z_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGU4501270.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22501..25367)
                     /gene="ycgV"
                     /locus_tag="ACHK0Z_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /pseudo
     CDS             complement(22501..25367)
                     /gene="ycgV"
                     /locus_tag="ACHK0Z_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(26208..27299)
                     /gene="ychF"
                     /locus_tag="ACHK0Z_RS06080"
                     /old_locus_tag="pgaptmp_001216"
     CDS             complement(26208..27299)
                     /gene="ychF"
                     /locus_tag="ACHK0Z_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU4501271.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27416..28000)
                     /gene="pth"
                     /locus_tag="ACHK0Z_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     CDS             complement(27416..28000)
                     /gene="pth"
                     /locus_tag="ACHK0Z_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU4501272.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28278..28556
                     /gene="ychH"
                     /locus_tag="ACHK0Z_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     CDS             28278..28556
                     /gene="ychH"
                     /locus_tag="ACHK0Z_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU4501273.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28611..30290)
                     /gene="dauA"
                     /locus_tag="ACHK0Z_RS06095"
                     /old_locus_tag="pgaptmp_001219"
     CDS             complement(28611..30290)
                     /gene="dauA"
                     /locus_tag="ACHK0Z_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU4501274.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30415..31362)
                     /gene="prs"
                     /locus_tag="ACHK0Z_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             complement(30415..31362)
                     /gene="prs"
                     /locus_tag="ACHK0Z_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU4501275.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31513..32364)
                     /gene="ispE"
                     /locus_tag="ACHK0Z_RS06105"
                     /old_locus_tag="pgaptmp_001221"
     CDS             complement(31513..32364)
                     /gene="ispE"
                     /locus_tag="ACHK0Z_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU4501276.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32364..32987)
                     /gene="lolB"
                     /locus_tag="ACHK0Z_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             complement(32364..32987)
                     /gene="lolB"
                     /locus_tag="ACHK0Z_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU4501277.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33201..34457
                     /gene="hemA"
                     /locus_tag="ACHK0Z_RS06115"
                     /old_locus_tag="pgaptmp_001223"
     CDS             33201..34457
                     /gene="hemA"
                     /locus_tag="ACHK0Z_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU4501278.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34499..35581
                     /gene="prfA"
                     /locus_tag="ACHK0Z_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             34499..35581
                     /gene="prfA"
                     /locus_tag="ACHK0Z_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU4501279.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35581..36414
                     /gene="prmC"
                     /locus_tag="ACHK0Z_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             35581..36414
                     /gene="prmC"
                     /locus_tag="ACHK0Z_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4501280.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     THLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36411..36803
                     /gene="sirB2"
                     /locus_tag="ACHK0Z_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             36411..36803
                     /gene="sirB2"
                     /locus_tag="ACHK0Z_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU4501281.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36807..37616
                     /gene="sirB1"
                     /locus_tag="ACHK0Z_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             36807..37616
                     /gene="sirB1"
                     /locus_tag="ACHK0Z_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU4501282.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37652..38506
                     /gene="kdsA"
                     /locus_tag="ACHK0Z_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             37652..38506
                     /gene="kdsA"
                     /locus_tag="ACHK0Z_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU4501283.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38655..38762)
                     /gene="ldrA"
                     /locus_tag="ACHK0Z_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             complement(38655..38762)
                     /gene="ldrA"
                     /locus_tag="ACHK0Z_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4501284.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39190..39297)
                     /locus_tag="ACHK0Z_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     CDS             complement(39190..39297)
                     /locus_tag="ACHK0Z_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4501285.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         9751 a         9828 c        10117 g         9645 t
ORIGIN      
        1 ttctattcta tttttttgcc gggtgatatt tttcatttct gctggatgag cgtcgtcgcc
       61 agaaggccac gtgagcacag gataagagaa cgaaaaatca gcagtctatg cagcgacaaa
      121 tattgatagc ctgaatcagt attgatctgc tggcaagaac agactactgt atataaaaac
      181 agtataactt caggcagatt attatgttgt ttatcaagcc tgcggatctc cgcgaaattg
      241 tgacttttcc gctatttagc gatcttgttc agtgtggctt tccttcaccg gcagcagatt
      301 acgttgaaca gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc gcgacttact
      361 tcgtcaaagc aagtggtgat tctatgattg atggtggaat tagtgacggt gatttactga
      421 ttgtcgatag cgctattacc gccagccatg gtgatattgt catcgctgct gttgacggcg
      481 agtttacggt gaaaaaattg caactacgcc cgacggtaca gcttattccc atgaacagtg
      541 cgtactcgcc cattaccatc agtagcgaag atacgctgga tgtctttggt gtggtgatcc
      601 acgtcgttaa ggcgatgcgc tgatgtttgc tctctgtgat gtaaacgcgt tttatgccag
      661 ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc tatcgaataa
      721 tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg ctgggtgtta aaatgggcga
      781 tccctggttc aaacaaaaag atctgtttcg tcgctgtggc gtggtttgct ttagcagcaa
      841 ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg acgctggaag aactatcgcc
      901 ccgcgtcgag atttacagta ttgatgaggc attctgcgat ctgacaggtg tgcgtaattg
      961 tcgcgatctg actgattttg gcagagaaat tcgcgcaacg gtgctacaac gtacccatct
     1021 tactgttggt gtggggatcg cccagaccaa aacgttggct aagcttgcca atcatgcggc
     1081 aaaaaaatgg cagcggcaga cgggtggggt ggtggattta tcaaatctgg aacgccagcg
     1141 taaattaatg tctgctctcc ccgtggatga cgtctggggg attggacggc ggatcagcaa
     1201 aaaactggac gcgatgggga tcaaaaccgt tctcgatttg gcggatacag atatccggtt
     1261 tatccgtaaa cattttaatg tcgtgctcga aagaacggtg cgtgaactgc gcggcgaacc
     1321 ctgtttgcaa ctggaagagt ttgcaccgac gaagcaggaa attatctgtt cccgctcgtt
     1381 tggtgaacgc atcacggatt atccatcgat gcggcaggcc atttgtagtt acgctgcccg
     1441 ggcggcggaa aaacttcgca gcgagcatca atattgtcgg tttatctcca catttattaa
     1501 gacgtcacca tttgcgctca atgaacctta ttacggcaat agcgcgtcgg taaaactgct
     1561 gacgcccact caggacagca gggatatcat taacgctgct acgcgatctc tggatgccat
     1621 ctggcaagcg ggccatcgct accaaaaagc gggcgtgatg ctgggggatt tcttcagtca
     1681 gggagtcgcg cagctcaatt tattcgatga caacgcaccg cgccccggga gtgagcaatt
     1741 gatgacggta atggatacac tgaatgctaa agagggcaga ggaacactct attttgccgg
     1801 gcaggggatc cagcaacaat ggcagatgaa gcgtgccatg ctttcaccac gttatacaac
     1861 gcgaagttct gatttactgc gggtcaaata aatatagcgg caggaaaaaa gcgctcccgc
     1921 aggagcgctg aagggattag cgaccgaaca ggtcacgttt tttcgcttta aacggctggg
     1981 aaatcaccac cagcaccgcg acaatcaggt aagcgataaa aataccgagc agccactgcg
     2041 gcatttccag acctaaaaaa tcccactgac gctcggcaca atcgccagag gcgacaaaca
     2101 cttgcggcac ccatttatcc agcggcagcc attccgggaa acgaaccata aaatcacagg
     2161 tggcaaacgg cgaaggatag agctgaagca tggtgtgctc gtaagttaac tgcacaccgc
     2221 ggaacgcact atacaaccag ataaccatcg ctacataacg cagcggagtt ttcggggcga
     2281 tcgcgccaat cagcgcagca cccagaacgc cgaataacgc acaacgttca taaatacaga
     2341 gcacgcaagg tttcagtaac atcacatgct ggaaccacag cgccgtcagt tccagtgcca
     2401 gagcagtaaa cgccatcaac agccacgcgc cccggccttg tgaacattgg ttcaaaaatc
     2461 gcaacataat catttccctg caatatgcat agagtgcgca gtttaaacca attcattcgc
     2521 tgcgccacca ggaggcagca aaaataggaa gcaattggac aattatcggg cgaaaaggca
     2581 aaccgggcat ttatatgccc ggtaagttgt tatcaaagcg ttgctatcca gcccatttgc
     2641 ataaaccatt cggttacagg ggcaagcgta aactcgacgc agagcaagcc gacgagtgtc
     2701 agtacgaggg tgtaaggcag ggccatccac accatgcggc cataagagag gcgaatcaat
     2761 ggcgcgagtg cagaggtcag caggaacagg aacgcagcct gaccgttcgg cgtagcgacg
     2821 gagggcagat tggtaccggt attaatggcg accgccagca gctcgtattg cttcaacgtg
     2881 atagcgccac tttccattgc cgctttcgct tcgttgatat aaatcgtccc cacgaagacg
     2941 ttatccgaaa tggatgacag cagaccgttg aaaatataga acagcgacag ctgagcatgt
     3001 tccgatgcct gcaacacaaa ctgaataatt ggcgaaaaca gttgttggtc gataatcacc
     3061 gcgactaccg agaaaaacac cgtcaacagt gcggtgaatg gcagagattc ggtgaaggct
     3121 ttaccgatag catgttcatc ggtgacaccg gtcaatgatg tcgccagaat aatgactgac
     3181 aaaccaatca agccaacttc cgccagatgc aacgccagcg cagtcaccag ccagacgcca
     3241 ataatcgcct ggacaatcag acggatttta tcctgacggg tacgctggtg gcggctttga
     3301 tcgtcaaact gttgcaacac ttcgcggact ttctccggca gcgtttcacc gtagccaaac
     3361 caacgcagct tctctaccag caggcaggtt aacaggccac aaatcagaac cggaacggtc
     3421 accggcgaca tgcgcaggaa gaaatcgcca aaatgccagc cagccgcttt agcgatgatc
     3481 aggttctgtg gttcgcccac catggtcatc acgccgccta atgcagtgcc gacaccggca
     3541 tgcatcatca ggctacgcag aaagccacgg aactgttcca gaaccacttt gtaatgcttg
     3601 tcgatatgac tatcgtcttg caggtcggta tcttcggtac gggaagaggc tacgcggtga
     3661 taaataccat aaaaaccgac agcaacgctg atcaccaccg ccacgacggt taaggcatcg
     3721 aggaacgcgg agaggaacgc tgccgccacg caaaaagaga gcgacagcag cattttggag
     3781 cgaatgctta acagcaaacg ggtaaatatg aacagcaaca gctgtttcat aaaatagata
     3841 cccgccacca taaacatcaa taacagcaag acttcaagat ttgccgccac ctcttcacgg
     3901 acgtgttccg cgctggtcat gccgatgaat accgcttcga tagccaacag accaccgggg
     3961 agcagcgggt agcatttcag ggccatcgcc agagtgaaaa taaattccgc taccagcaac
     4021 cagcccgcga cgaaagggct gatgaggaaa attaacgggt ttacgattaa gaaaatgatg
     4081 agggcgagtt tgtaccagtc gggggactgg cccaaaaaat tgcgccatag cgcgcggccc
     4141 caggagatct ccatgatggt ttcccttacc ttacaaataa tcaatgatgt ttttatgttt
     4201 aaacgcaaag cttaacggtc aggcaggagt gaggcaagtc ttgatagtca aggggaaaga
     4261 gatgcggaaa atgaagcctt gatccctttt ttcttctttt tgtctgctat cagcgtagtt
     4321 agccctctgg tatgatgagt ccaactttgt tttgctgtgt tatggaaatc tcactatggt
     4381 cattaaggcg caaagcccgg cgggtttcgc ggaagagtac attattgaaa gtatctggaa
     4441 taaccgcttc cctcccggga ctattttgcc cgcagaacgt gaactttcag aattaattgg
     4501 cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg gcacgagatg gctggttgac
     4561 cattcaacat ggcaagccga cgaaggtgaa taatttctgg gaaacctctg gtttaaatat
     4621 ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg cagcttattg ataatttgct
     4681 gtcggtgcgt accaatattt ccactatttt tattcgcacc gcgtttcgtc agcatcccga
     4741 taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg atgcctttgc
     4801 cgagctggat tacaacatat tccgcggcct ggcgtttgct tccggcaacc cgatttacgg
     4861 tctgatcctt aacgggatga aagggctgta tacgcgcatt ggtcgtcact atttcgccaa
     4921 tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt tgtgcagtga
     4981 aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga gtggcgagat
     5041 ttggcaccgg atgcagaaaa atctgccggg tgatttagcc attcaggggc gataatccct
     5101 tccgtttaaa gagcaaaccc ctcaaacgag gggttttttg ttgtttttac agatttccca
     5161 ttcttggcgg gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc tgttcgagca
     5221 tcacatcaaa tccccacagg cgatgcacat gcttcagcac ttctttgcgc ccccgatcca
     5281 gcggtgcgcg attatgtgga atgtaacgca gcgtcagcga acggtcgccg cgcaaatcca
     5341 cgttccagat ctgaatattc ggctccagat tacttaagtt atattgcgac gataaccggt
     5401 tacggatctc ccgataacct tcttcattat gaatagcgga aatctccaga taattatgcc
     5461 gatcatcatc cagcacggtg aagaagcgga aatcacgcat cactttcggt gacagaaact
     5521 ggctgataaa actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt tccagccagt
     5581 cggaaccggc gatatccggg aaccagtatt tgtcttcttc cgttggcgac tgacaaatcc
     5641 gtttaatatc ctggaacatg gcgaacccga gggcatatgg gttgatgccg ctgtaccacg
     5701 ggctgttata ggggggctgg aagaccacat tggtgtggct gtgcaaaaac tccagcataa
     5761 aacgttccgt tactttccct tcatcataca gatggttaag gatggtatag tgccagaagg
     5821 tcgcccagcc ttcgttcatc acctgagtct gtttttgcgg ataaaaatac tggctcacct
     5881 tacgcacaat acgcaggatt tcacgctgcc atgattccag cagtggcgca tttttctcca
     5941 taaaatagag caggttttct tgtggttcgg acggatagcg gcgcgcttca gcaaccgttt
     6001 tctcttcctc gcgcttcggc agggtacgcc agagcatatt gacctgactt tgtagatact
     6061 cttcgcgact tttctgccgg gctttctctt cttgcagcga gattttttgc gggcgtttgt
     6121 accggtccac gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt tctacttcat
     6181 caacgccata acgttcttcg cactcggtaa tatatttacg ggcaaaaatc agataatcga
     6241 caatcgaact ggcgtcggtc caactgcgga ataagtaatt gtttttaaag aaagagttat
     6301 gcccatagca ggcgtgtgcc atcaccagcg cctgcatggt aatggtgttc tcttccatca
     6361 ggtaagcgat acacgggtta gagttaatga cgatttcata ggccagtcct tgctgaccgt
     6421 gcttatacag ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc ggatagttaa
     6481 ttggcatgcc gacgctggag taggcatcca tcatctgttc tgaggttatc acttcaatct
     6541 ggtgcgggta ggtatccagc cggtagagtt tcgccacccg gtctatctct gccagataaa
     6601 catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg tccttattca
     6661 tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg tgtggagcac
     6721 ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc cggagaattt ttttctttgc
     6781 gatttcttat tatcagagtg ccactaatcc gcttctgaac ggaattttat gctggataaa
     6841 aagggcgttc agcaggagat actaaagacg ccatattgcc gcagagtcag ggagatgtga
     6901 gccagctcac cataaaaaag ccgtatgttg aataatattt tcaactgagt tatcaagatg
     6961 taattagatt attattcttt tactgtatct accgttatcg gagtggctat gcgagttgtc
     7021 atactgggaa gtggtgtggt aggcgttgcc agcgcctggt acttaaatca ggcaggacat
     7081 gaggtcaccg tcattgatcg ggagccgggg gccgccctgg aaaccagcgc cgcgaatgcc
     7141 gggcaaatct cccccggata tgctgcaccg tgggcggcac caggtgtgcc tttaaaagcg
     7201 atcaaatgga tgttccagcg ccatgcgccg ctggcggtcc gtctcgacgg tacgcagttt
     7261 cagttgaaat ggatgtggca aatgttacgt aactgcgaca ccagccacta catggaaaac
     7321 aaagggcgga tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc attgcgcgcc
     7381 gaaaccaata ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt ccgtaccgaa
     7441 caacagtatg aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg cgtaccgtat
     7501 cagctgctgg aatccagccg cctggcggaa gtggagcccg cgctggcaga agtagcgcac
     7561 aaactgacgg gtggtctgca actgcccaat gatgaaaccg gagactgcca gctgtttacc
     7621 cagaatctgg cgcggatggc agagcaggcg ggggttaaat tccgctttaa tacgcccgtt
     7681 gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga agtgtggcga tgaagtgatt
     7741 aaggccgatg cgtatgtgat ggcgtttggt tcttactcga cggcgatgct caaaggcatt
     7801 gttgatattc cggtgtatcc gctgaaaggc tactcgctga ctattccaat tgcgcaggaa
     7861 gatggtgcgc cagtatccac tattcttgat gaaacctaca aaatcgccat tacccgtttc
     7921 gataaccgca ttcgtgttgg cggaatggcg gaaattgttg gttttaacac cgagctgttg
     7981 caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg cggcggtcat
     8041 gtcgagcagg cgactttctg gacgggtttg cgcccgatga cgccagacgg tacgccggtt
     8101 gtcgggcgta cacgctttaa aaatctgtgg ctgaataccg gtcacggcac gctcggctgg
     8161 acgatggctt gcggttccgg tcagttgtta agcgatctgc tctctggtcg cacgccagcg
     8221 atcccatatg aggatctaag cgtagcgcgc tacagccgtg gatttacgcc atcacgtccg
     8281 ggccatttac atggcgcaca cagctaagga aacgagatga cccgtccgat acaggccagc
     8341 ctcgatctgc aggcattaaa acagaatctg tccattgtcc gccaggccgc gccgcacgcg
     8401 cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg gtattgagcg tatctggagc
     8461 gcgctcgggg ccaccgatgg ctttgcatta cttaacctgg aagaggcaat aacgttacgt
     8521 gagcgcggct ggaaggattt ttccatgctc aggatctgga gatttatgac cagcaccgcc
     8581 tgaccacctg cgtacacagc aactggcagc tcaaagcact gcaaaatgcg cggctaaaag
     8641 caccgttgga tatttatctt aaagtgaaca gtgggatgaa tcggttgggc ttccagcccg
     8701 atcgcgtgct taccgtctgg cagcagttgc gggcaatggc gaatgttggc gagatgaccc
     8761 tgatgtcgca ttttgccgaa gcggaacatc ctgatggaat ttccagcgcg atggcgcgta
     8821 ttgagcaggc ggcggaaggg ctggagtgtc ggcgttcgtt gtccaattcg gcggcgactc
     8881 tgtggcaccc ggaagcgcat tttgactggg ttcggcctgg cattattttg tatggcgctt
     8941 cgccgtccgg tcagtggcgt gatatcgcca ataccggatt acgtccggtg atgacgctaa
     9001 gcagtgagat tattggtgtc cagacgctaa aagcgggtga gcgtgtgggc tacggcggtc
     9061 gctatactgc gcgcgatgaa cagcgaatcg gcattgtcgc cgcagggtac gccgacggtt
     9121 atccgcgcca cgcgcctacc ggtacccctg ttttagtgga cggcgtgcgc accatgacgg
     9181 tggggaccgt ctcgatggat atgctggcgg tcgatttaac gccttgcccg caggcgggga
     9241 ttggtacgcc ggttgagctg tggggcaagg agatcaaaat tgatgatgtc gccgccgctg
     9301 ccggaacggt gggctatgag ttgatgtgcg cgctggcgtt acgcgtcccg gttgtgacag
     9361 tgtaacttgt tgtaagccgg atcggagaca gcgtcttctg gttgcgaaaa aatcatccat
     9421 ccggctggtc agcaactgta gttgttaatg tgacagagcc attgcccatg atagtgtcca
     9481 ttaaaagaat ggacactatt tccccggaac ctgaactcac cgcacaggcg ttctacataa
     9541 aacgcttacg cttcattgtt gactcgaact cgacttcaga taaatcacgc tttacccttg
     9601 atggagcctg tacatagatt tgtgtaattg cctgattttg atatgttcaa ttcaacatca
     9661 aatgaaggtt aaattatgga cgacaaacaa ttgcaggctc aggctgcgtt cagcaaagca
     9721 tcgcaaccgg cgatagatgc ttcattaaat ttaagattca gcttcctctt cagccacccg
     9781 tacgccaatc ttcaacactt cattatcttc tttctcggcc accgtccaga tcatcccggc
     9841 aaactctacc tggtcaccga caaccggtgc cgcgcctaac aactgctgga caatttcacc
     9901 cagcgtttgc tgcttatcac gatattctcg cccgtcttcc aggccatata tcagcgcaac
     9961 atcagcatat ttggcgctgg cttcgagaat gaagtcacca aagaagcgtt gatccagcgc
    10021 gaccggcggc gactggctga acagtttacc gagcgccgga agatcgcgtt cccgaccaat
    10081 tacacacaac acatcgcctt cgcgcagtcg ggtgctgccg gtaggatgaa gcaactggtt
    10141 atcacgaaac agtgccgcaa tacgcgtctc ttttggcata tgcaaatcgc gcagtgccgc
    10201 gcccacgcac catttatcgg cactcaattg ataaacaaac tgctcccacg gattttccgg
    10261 atgaatatcc aggccaacgc gtgacaccgg acgtcccact ggcggaacca ccactttggc
    10321 ttttttagcc gcccacgaga gtgatgttcc ctgcaagagc agtgaaacca gaaccacaaa
    10381 gaaggcgaca ttaaagaaca gacgtgcatt ctccagcccc gccatcatcg ggaacacggc
    10441 caggatgatt ggcaccgcgc cgcgtaatcc tacccagctg ataaacacgc gctcacgcag
    10501 attgaaaccg cggaagggga gcaatccagc aaataccgaa aggggacggg cgaagaatat
    10561 catccatgcc gacaaaatga gcgccggaat ggcaataggc agcagatcgc ttgggttaac
    10621 caatagcccc agcaccagga acatggcgat ttgcgccagc caggcgaggc cgtcgaaatt
    10681 ttgcaggatg ccgtagcggt tgcgaatcgg ccgattaccc agcagaaaac cgcacagata
    10741 aacagccaga atgccgctgc cttccagcgc cgtggttaaa gcaaaaatta aaataccgcc
    10801 gcttaatgcc agcaatggat ataatccggc aggcagggcg atgcgattaa tcatttgcag
    10861 cagtaaataa ccaccgccaa gtccgatgac aatcccgagg ccaaattgct gcagaatatc
    10921 gaccacgaac atccaactga cactgctttc atgttgctgg atcatcgcaa tcagggtaat
    10981 cgtcagaaag accgccattg gatcattact gccggattca atttccagcg tcgagccaac
    11041 acgttcgtta agccccttac cacccagcag agaaaagacc gctgcagcat cggtagagcc
    11101 gacgatagcg ccgattaata agccttcaat caaatcaaga ttaaacagcc acgccgccat
    11161 catgccggtt aaaccagagg tgataagcac gcccagcgtc gccagcgaca gtgccggtcc
    11221 taacgccaca cgaaaggaac tggcctgagt gcgcatcccg ccgtcgagca aaataatcgc
    11281 cagtgccagg ttacttacca tgtaggcgaa ggggtaatta tcaaacggga taccgccgac
    11341 gccatcgact cctgccagca tgccgatcgc cagaaaaata accagaatag gaatgccaag
    11401 acgggaagaa aatgaactaa gtaatatact gctggtgaca aggatggatc ctaagatgaa
    11461 aaggctaatt attgttgtgg catccaacgg tcggttactc ctgattacgc tgtctcttat
    11521 ataaacccta ccatattagc ggcagagaca gcgttttact tagccctgaa gcgtgatttt
    11581 tttacatttt aagaacagga tgaccgctaa tcgtcaactg agtgccttcc cgggtgttat
    11641 tcagaatggc atgtgcgccc aacggcagcg taaccgtgcg ttgttcatga ccaaaatcga
    11701 ggccggtaat gagtggaatc gacaggcggg aacgcaaaaa cgcgtacact gactccaggt
    11761 tgtaacctgc gtcataatca ttgggcgtgc tgccgctaaa gctaccgaga ataatcgcct
    11821 tctgacgtgg caaaattccc gcatgataga gctgcaacag catacgttcg acgcggaaag
    11881 ggtgctcgtt aatatcttcc agcaccagaa taccgttctc aatttttggc atccacggtg
    11941 taccaatcag tgaaatcagc atcgcaagat tgcctcccca caacgtgcct tcggtctgac
    12001 atgtcgggcc ttccccttgc cattcaatgg tgaaggtttt attgcgtaac gccagccaga
    12061 aatggtgctc ggtaaaggcg ttcagttcat ccgcaccaaa gtttgctacc agcatggggc
    12121 cgctaaaggt gatgacattg ccttgcgcca gaagaccgca ctgaatggcg gtaaaatcgc
    12181 tatgtccgca aatgagtaac gggtcatgtt gttggcgagc caccagtgcc tgccagtcaa
    12241 tatctgccag taaacgactg gcaccgtaac cgccgcgtac agccagcacg atggtgttgg
    12301 gggtagtcag tctggcaagg gaattgagat cttccaggcg ctccgtttcc gtacccgcaa
    12361 aacgctcaca acgacgggca ataacctcga cgttattgac ctgatgcccc tcgtcggtaa
    12421 ggcgatggat accacgcagc gcggcgtgct gtttaatgca gtaacccgat ggggcaatta
    12481 agtgaaacag agacatggca attccttgct gacaacagaa acgaaatgta tatcatgccg
    12541 cttaggtgtg ccgttgtcac ctcaacggcg attccaggct ataaggatag aagaagtgaa
    12601 attgagatgg tttgcctttt tgattgtgtt attagcgggt tgttcatcaa agcatgacta
    12661 tacgaacccg ccgtggaacg cgaaagttcc ggtgcaacgt gcgatgcagt ggatgccaat
    12721 aagccagaaa gccggtgcag cctggggcgt cgatccacaa ttgatcacgg cgattatcgc
    12781 tattgaatcg ggtggtaatc cgaacgcggt gagtaaatcg aatgccattg gtttgatgca
    12841 gttaaaagct tcaacctccg gacgtgatgt ttatcgccgt atgggctgga gtggtgagcc
    12901 gacgaccagc gagctgaaaa atccagagcg taatatttca atgggggcgg cttacctgaa
    12961 tattctggaa accggcccgc tggcaggcat tgaagatccg aaggtactgc aatatgcgct
    13021 ggtggtgtca tacgctaacg gggcaggtgc gctgctacgg actttctcgt cagatcggaa
    13081 aaaggcgatc agcaaaatca acgatttgga tgctgacgag ttcctcgatc acgtagcgcg
    13141 aaatcatcct gcgccgcagg ctccgcgcta tatctacaaa cttgagcagg cactggacgc
    13201 gatgtaaatc agtcgcgcac tttgtccgct ttttctcggg cttctcgctc gagagagaaa
    13261 ataatccgct gtaattgccg ctccaccgtc gggctgacgt taagaaaacg gaagctcaga
    13321 cggggagtgg tgatggtttc attcttgcca tcaatcactt tgcgctcgct gatggagatt
    13381 aactgggcgt caaagtgaaa aacaccccat tgccccatgt tgacttcaat ctgagcgaag
    13441 cgcatgcctt cttgtaattc ggcaggcttt gctgtttcca gtaatgcgcc catgccgcct
    13501 aacgacaaat catacaggcg gaaacgtaac gtactgttat ccgccagttt ggtctggcaa
    13561 aaataaggcg gatggagtgg ggcggagatg cggaaatatc ggcgtcgttg tacaaaccat
    13621 aaggtgggag gcggtacggt gataaatgcc ggaagctgca agtattcact ctgctgtagt
    13681 tgttcaacag taaactcgac tttcgcaccc tgagtttcgg cggtaatggt aatgtgctgt
    13741 gcctttagca cggcgatgtt gtcttcggct tgactgccga aatccagcac cagtttatcc
    13801 ggggttattg ccagtaattt gctgatcagc tgcccgccat tccaactgag acgcaaagga
    13861 attgcggctt tgtgcaaatc gcgtaacacg cccaggacgg ctaacggatt ttgtttcagg
    13921 aactgctcat ggtaatgact cacgcggaaa aactcctgac tgacaaactg tctttggttt
    13981 atcggtcaca gttaacaaaa cttaatacaa atgcgtgaat attttttaca tgttgttctt
    14041 aaatcagccg acatacgccc agcatcgatc ccgcccttgc ctatacttag agcgttgatg
    14101 taagcatttc ttgcgtccga ttcatcgaaa cgagggcctg aacatgggaa ttattgcctg
    14161 gattattttt ggcctgatag ccggcattat cgccaagcta atcatgccgg ggcgtgatgg
    14221 tggtggattt ttcctgacct gtattctcgg gatagtcggt gcggtggtcg gcggctggct
    14281 ggcgaccatg tttggcattg gcggctccat cagtggtttt aatctgcaca gcttcctggt
    14341 ggcggtggtg ggagctattc tcgttctggg catattccgc ctcctgcgaa gagaataaga
    14401 ttttcataag gcggatagcg atacagatgc cgctatccgc tttcacatca gaacgtatac
    14461 tcgacacctg ctttacgggt gaaaaaaatc aatatagcac ttcgatatcc gataaagtcg
    14521 gttcccggta acaacaacag aatccttttt taattattgt ttcgttgttt ttggtgtgat
    14581 tcagaaaata aatattttag acagtagcca gtcaggcgtt aaaaatgggg cggatagtga
    14641 ggcggttttg gctgcgctta ttgaacattt agaattgatc aatccttctg gcagattaag
    14701 ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag gaatggtctg aattcttttt
    14761 ttggttgcta aattctctgg agtgtctgga ttatgtgatt attaatttaa ctccagaaag
    14821 caaaaaaacg ttgatgagtg aacaccgtaa caatattcag gtagcaattg atgctttata
    14881 tagccaacgt cggcgtaaat cgccgggtga tgaaagtgaa acacttaccc gtagaaatga
    14941 cgctatcttc ggcaaccacg tgtggcaaac atttgcgcaa tacttcccgc caggacttga
    15001 aaaaccgtct gtgtagaaaa aaccatagct atggtgttat ctgccgacaa cgccatccgg
    15061 agaagccgaa tgacgttgtc ggagtaagcg ctggttaagg tgtgggttgt gcctctttgg
    15121 ttgagggttg cgtcgttgct gacttaacgg tcggacgcgt cgccggaaca ttgtcacacg
    15181 gttgctcttt cgggcagatc aaatccagca ttttcagcgt cacgccattg gtccagccaa
    15241 agccatcctg taatggatat tcgccaccgc cgccccccgt tccggtggtg ctgacatcat
    15301 atttttccac cagctttttc tcccggtcat aggtgtgctg aacattggtc aggaagtgcc
    15361 agctaatgtc catcgccacc tctttttgcc cgtagttttg taatccttct gtcgcgaccc
    15421 actgtaacgg tgcccagcca tttggcgcat cccattgttg cccacttttc accgacgtgg
    15481 tgttcaggcc gccgggttgc agcagatgtg ttttcgtcgc cgtcgccatt ttgttggcgc
    15541 gatctttcgc tgccgcattg acgtacagcg ggaacagggc ggccgcggtt aactgattgc
    15601 gcactttatg acttttcagg tcgtaatcgg cataccagcc ttgttgatcg ttccacatgt
    15661 atttttcgat ccctttttga cgggcatttg ccagcgtttc gtactggttt gccatcgcgt
    15721 tatctccggc agctttgctg gcgcgggcga ggattttttc cattttaaac atcaggctgt
    15781 tcagatcgac cggtacgatg ctggtggtgc gtaaggtatt tagttgatgc gggttgtcca
    15841 tccagcgcga gctgaaatcc cagccagacg cagcggcaga gcgcaggtcg cggtaaattt
    15901 cagtggcagg tcgattcgga ttgcttttgg cggtggcaat atcttccacc catgactctg
    15961 gtcgtggcgt atcgcgatcg tcccagtagc ggttgagaag ggtaccatcc tgaagtttta
    16021 caacgcgttt ttcctgttgt ccggcttgca ggttttcaac accgtccatc cagtaagcat
    16081 attctttttg catttgcggc aggtattgct tcaacgcggc atcgccttca tgctgcgcca
    16141 gtaactctac catcagggca aagaagggcg gttgcgagcg gcttaaatag taactgcggt
    16201 tgccgttggg aatatgaccg taagtgtcta tttcatgagc aaaattggcc accatatccg
    16261 cgactttatc ccagtgaccg ctttcggcaa gtcctaacat ggtgaagtaa ctgtcccagt
    16321 aatatacctc gcgaaaacgt ccgcccggca cgacataagg ttccggcagc ggtaacagag
    16381 aatcccattt ttcggtgttt tcggtagaac gcgttaatac cggccaaagt ccgtcaatat
    16441 gttcgcgcag tgactgcccc tctggcggaa catatttctc gccttctttc ggcagggtga
    16501 aattgacgtt aacgaaatgg cgcagatcaa atccgctctg gttttgctgc atccgataat
    16561 cagcaaggat catcagcgga tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg
    16621 gaaaaagttt ggcgttttgc acatcattaa acagcggccc taataaaata tcaggcggct
    16681 gtggtgttac cggtgtttct tctgcctgca ccgatagcgc agcgaaacac aaaaagatac
    16741 aggctggaat taacgccatt ttttgcgggc gagaaggtgc gggggatttc atcaatcatt
    16801 ctcctttggc gaaaccgaat aaaacggtat tcaagtatca gaaaacctta gttcaggatc
    16861 gcactagctg catgatcgaa atctcattct gcgaactgtc aggcatttta gaaaagggaa
    16921 ttaaagggat aaataacggc gaaatagagt gatcaaataa cgtctgttta ttagccagcc
    16981 agcggcgttg ggttcgtgat tagcgaagaa aaatcatcat aaattggtga aaaatataac
    17041 aggtagttaa aaaccattag tgctgagtaa attgccggat gacatcagaa cgatgccatc
    17101 cgaacagtgg cttaaccctg acggttgaaa cgttgccttt taacgtccag cgttagcgtt
    17161 tcttctggtt gtatcgcata cagtttctca ccctgctggc aaatccagcc aatccccagt
    17221 tcacgggcga ttagggcgct gtgggatacc ggacttccgg cgctaaggca gatacctttt
    17281 acaaccgccg gatccagttg cagtactgtg gaaggataaa tgttctccgc cagtagaata
    17341 gttggcgagt taaactgcgg gagttcttct ttcgtttggg tcaggtggac cagggtgcga
    17401 tgcagaagat cgtccacatc aatatagcga gcttgtagat attcatcatc cagttgctgg
    17461 tattgctggc taagttcttt aagaacttgc tgccaggcat attctgccgt gcaatgttca
    17521 tgctgaagga gttcgcttgc cgccgccagc agttccggat catctaacag tgtatggtga
    17581 ccagaaaaga ttgcggcaat atcgtcaagc ccgctggctt ctgcttttgc tgttaacgtc
    17641 atcagatcta ataacgtgaa gtcaatagcc tggcgtaatc gatcttgttc ttcttccacg
    17701 gtcagggttg attttgcctg taccgtacat aaaactggtt gataataaaa ggctttaccc
    17761 gaaacaggcg gaacgggacg cagagtaggt ggagcgactt cctccgtttc accaaagtta
    17821 tcttcagcca gctgacggaa agcgatcagt gcctcttcag cttctggccc tttcgcaatc
    17881 aggcgcagcg tatcgttata gcgaacttgt agtaacgcaa tctggttaat actctctggt
    17941 gtgacgcatt tgccgttttt ttccagcaac atatcggcat taaatgtcga taaggtataa
    18001 accagccggg aggccggacg tacatgcagg ccgttacggt tttttatgac caccgccaga
    18061 gaacgggctt cttcatcgta cgcaggacat gtgtcagaga ttttagtgtc ggaggacggt
    18121 aaacccagtt gttcacgttt ggcttccagc gcatgcatag cgtcaaagat aactttgtcg
    18181 atatccgctc ccgaggccgc gctgaccgtt gctgccagtg taccttcgac caacggcgca
    18241 gcacacaaac gtacttttgc ggcgatcttg ggagccagca attccagcgc agtttcagca
    18301 ctcaataatg cgctacccat atccatcatg accagcacat ggtcggcatc agcaacagat
    18361 tcgatggcct ccatcacttt gacggcatcg gtaccaatag gattttgtgg atcgtcaatt
    18421 cccgcggcaa tggcgatttt acaactatca ctcattaaca tctgacgggc taattcaccg
    18481 acaccttctc ccagtcggct gctatgtgaa actatgacca ggtttaccat caccaattcc
    18541 ttactctttt gcggctaacg ccaacatttg catcataaac atcaccgagg tcgcgccggg
    18601 atcctggtga ccaatactgc gttcaccgag ataactggcg cggcctttgc gggcttgcat
    18661 cgtaatcgta ctttgtgcag cggattcggc gattctactg gcagcttcga gcgccaccgg
    18721 aacagagaga ttttgctcgc tggactgacg taacgattcc accaccggca cccacacatc
    18781 acacatggtt ttatcgccag gttcggcttt cccgcgactg attacgccgt ccacgccatc
    18841 gcggaacatc tgataaagct cttccagtgt caggctttgc cgtgcctggg tcgcctgtgc
    18901 ggcgcggata aagaaggtac cgaacagcgg accactggca ccaccgacgc tggaaagcag
    18961 cgtcataccg gtattcttga gaatgaaacc gatatcttta tctgcgatag cagggagttt
    19021 ttccaccact ttgctaaagc ctcggttcat atttagcccg tggtcagcgt cgccaatttc
    19081 gcgatccagt ccggtaagat actcgctctc ggtgctgaaa atatcgccac aacgagtgag
    19141 ccagttaaca atttgagttc tgctcagtga cattgctttc tccttattta ccccagttaa
    19201 gggccggggt gtggaccggg gcgtcccaga gtgccagcgt ttcgtcatca actttcagta
    19261 aggtgattga gaaaccggtc atatccagtg aggtgcagta cgcgccaatt aaattacgtt
    19321 cgatagtcaa tcccgcttgc tggcaacgtg tggtcaggcg gttatagacg ccgtacagct
    19381 cagaaagcgg agttgcgcca agattgttaa ccagcgcaat cacccgatcg ccagactgga
    19441 gcggttgttt ggtttgttgt tcttcctgcc aactgccttg ttgataatcc cagaaacgca
    19501 aagtgcgatg gtatgagcca ttttccagca gggtgtcgaa catttcatcg acggtttgat
    19561 caagggaaga gaaggagcgg cggtcaatac ccggctcacc atgaatgccg acgccaaact
    19621 ccatctcatt atccgccagg gtaaaagaag gtttgcccgc ggcaggaacg gtacaggcac
    19681 cgagagcgat acctattgag tggccttgat tattcaactt acgccccagt tccgcacagg
    19741 cgtccagtga gtcgccacgc tccgccgctg cgcctacgag tttttcaatt aatacggtgt
    19801 tggcaacgcc gcgtcgcccg gcagtataaa gactgtcttt taccgcaacg tcgtcatcaa
    19861 tgaccacagt ggtcactttt acgcggctat cgtgcagtaa ctcggtcgct gtttcaaagt
    19921 taagaatatc gccggtgtaa tttttgataa tcatcagtac accttcgccg ccatcaattt
    19981 gcatggcgca ttcaaagatt ttatcgggcg tcggtgaggt gaaaatttcg cccggacagg
    20041 cccccgaaag catcccctga ccgatataac cacagtgcat cggctcgtgt ccgctgccgc
    20101 cacccgacag cagggcgact tttcctgcaa cgggggcatc agctcgggtg acatacaccg
    20161 gatcctgatg cagtgtcaga gatggatgcg ctttcgccag tcctgccagt tgttcgtcca
    20221 gtacgtcttg cacatcattg atcaattttt tcattatttt gctccagcaa ttacggtagg
    20281 gcatggatga tgttcaacga cacggcgacc actgactgcc gatgaatcca ttgtgcatca
    20341 ggcaagggaa aagaaaatta gcccaaatta tgtttcatag tgaaacatat gctttaatga
    20401 atgttccata ttgaaacttt tacgtgtatt aatacttaaa attgcgagcc ggaacacctt
    20461 ttgtcataag ggatgcggga tatgagtggc gcttttaaca acgatggtcg gggcatatct
    20521 cccttaattg caacctcctg ggagcgatgc aataagctga tgaaacggga gacatggaac
    20581 gtaccacatc aggcccaggg cgtgacattt gcttctattt atcggcgtaa gaaagcgatg
    20641 ctgacgctcg gtcaggctgc gctggaagat gcctgggaat atatggcacc gcgagagtgt
    20701 gcgctgctta tcctcgatga aaccgcctgc attctcagcc gtaatggcga tccgcaaacc
    20761 ttgcagcagc taagtgcact gggattcaat gacggcacgt attgcgccga gggaattatt
    20821 ggtacttgtg cgctatcgtt agcggctatc tctggtcagg ccgtgaaaac gatggccgat
    20881 caacatttca aacaggcact ctggaactgg gccttttgtg caacgccgtt gtttgacagc
    20941 aagggccgat tgacgggaac aatagcgctg gcgtgtccgg ttgagcaaac taccgcagct
    21001 gatttgccgt tgacgttggc aatcgcccgc gaggtcggta atttactgct gacggacagt
    21061 ttgctcgctg aaactaaccg tcatttaaat caacttaatg ccctgttaga aagtatggat
    21121 gatggcgtga ttagctggga cgagcagggt aatttgcaat ttattaatgc ccaggcggcg
    21181 cgggtcttgc gccttgacgc gacggcaagt cagggacggg caatcactga actcttaacg
    21241 ttacccgccg tattgcaaca agcaataaaa caggcacatc cgctcaaaca cgtagaagca
    21301 acctttgaaa gccagcacca gtttattgat gcggtgataa cccttaaacc gataatagaa
    21361 acgcagggaa ccagctttat tttgttgctc catcctgtgg aacagatgcg gcagttgatg
    21421 accagtcaat taggaaaagt cagccatacc tttgctcata tgccacagga cgatccgcaa
    21481 acccgccgct tgattcattt tggtcgccag gcggcgcgca gtagctttcc tgtcctgctt
    21541 tgtggagaag agggcgtggg caaggcactg ctaagtcagg caattcataa tgaaagcgag
    21601 cgtgctgcag gtccttatat cgccgtcaat tgtgagttat atggtgatgc tgcgctggcg
    21661 gaagaattta ttggtggcga tcgcacggac aatgaaaatg gccgtctgag tcggctggaa
    21721 ctggcacacg gcggcacgct gtttcttgaa aagattgaat atctggcggt ggagttacag
    21781 tctgctttgc ttcaggttat caagcagggg gttatcacgc gactggatgc gcggcgttta
    21841 ataccaattg atgtcaaagt gattgcaaca acgaccgcgg acctcgcaat gctggtggaa
    21901 caaaatcgtt ttagtcgcca gctgtattac gcgctgcatg catttgaaat taccatcccg
    21961 cctctgcgta tgcggcgtgg cagcattccg gcgctggtga ataacaaatt acgcagtctt
    22021 gaaaaacgct tctctacgcg gctgaaaatt gatgacgatg ccctcgctcg cctggtttct
    22081 tgtgcatggc caggcaacga ttttgaactt tacagcgtca tcgagaatct tgctctgagt
    22141 agtgataacg ggcgcattcg cgtcagtgat ttacctgaac atctgtttac cgagcaggcg
    22201 acagatgatg tcagcgccac ccgcctttcc accagtctgt catttgcgga agttgaaaaa
    22261 gaggcaatta ttaacgcagc ccaggtcaca ggcggtcgca ttcaggaaat gtcggcttta
    22321 cttgggatcg gccgcactac gctgtggcgg aaaatgaagc aacatggcat tgatgcaggg
    22381 cagtttaagc gccgggtatg aaagacagaa acgatttctg atacatcaga gtgatctgta
    22441 tttcattccg gcgcacgcta acaattttca gcatcgttta agggcttgtc tatcccgcac
    22501 ttaaaagctg aagcgatatc ctccgttgac ttgcttctga tcaaagaggt taccctgcgt
    22561 gtaatccgct tcgagataga atgtgtgctg tttgttatac tgtgcactga ctcccacgcc
    22621 gttattccag ccattacctt tgaaactgta cttctcccgg gagttgttca cagatattcg
    22681 gtatcccctg aaaactcgcg gatagcgcca gtcttcacat agacattcag ctggctgttg
    22741 cctgcggtga tgtcataccc cagtatcatg ctggcacgcc ccagcagcga ttcgtagtga
    22801 ttcagatgta tattgaggcc attactcgcc ttcatagcca tctcattctg gtggctgtat
    22861 gtaagctggg tttgcggctc tatatagaac ccataaccag taggggacag gttgaacctc
    22921 tgcccggctt ccagggagat gctcattcca ttcgcagtgc cgttggcgtt aacgccgttg
    22981 ttctgactgt ccagtacgtg gaaactattt ttctggcgcg atgcttttat aacgagatcg
    23041 ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc ccatgtagtc tgaacgtgcg
    23101 gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg agccaatata cagaccgaca
    23161 tacaacggca ttacatcaga gagacgttta tccccaccaa actggatacc gctgtaaccc
    23221 atgtcaaagc cgctcagttt gccactggca aaggagtcca ggcttccccc ataactgcgc
    23281 aaccagatat taccgtcttt actctgattt cgcaggtcac ccatccgttg catcagcgta
    23341 cggttttcaa catagttcaa taagtaaccg acattgagat aattgccgcc agcatctgcg
    23401 gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag gatcggggtt gactatggga
    23461 ggctgagcgg gagctggtgt gggttcagga ttaggagtcg gttccggaac tgtcccggaa
    23521 gcgtaaagct cccagttagt gccattttta cgcacatcgt acagatatcc ccccaactca
    23581 acctgagaag acgcgctgaa cgaggccgca ccgtcagtgg tttttaccac tgtcagaact
    23641 tcatttcccg tagtggcctc gctgccctgg ttgcggatag ccaatacgtg attaccagca
    23701 ctacttccgc tgatattcaa taaatcccct ttattattaa cgccattacc ctcgccaaca
    23761 acatcagcac gcataataaa ggtactgtta ccgctcaggt tctctacgtt taatgtggca
    23821 aatgtgccgg cagttgaccc gtggctggca aaatcgacag ttgaatggct cagcgccagc
    23881 gtgtcgagat tagaattact tgttacgttc cagacgctgt tattcattgc aacgtccagt
    23941 ttcccgccat tgacattatc gctgagggag gaacctgtcc aaaccgaccc tgggtgcata
    24001 tctagattga ttagcccacc tttggaaaga acgctaccgt tgataagcat acgacccgag
    24061 gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg ttgcaatggc catctggtcg
    24121 ggtgtactca tatcaatgac cagatcgctc gtgaggtcga tctggctgtt ggtcatggca
    24181 taaattcccc tggctcctgc cgcgccggtg atagccaaac tgtctccggt tattctaccg
    24241 ccgctgagcg cccacaaacc cagcgccaga ctgccattac gatcaactgt aatatcggta
    24301 ttttgcatgt tgacaacagc cgttgccgtc tgggccgagg caccataaga accgccggaa
    24361 aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga ttgtcgcgtc tgaaccactg
    24421 gtgacgaggc caccgccctg cgcggaagaa atatggctat ctgcaccgat agtggttgta
    24481 ccaccacgaa cttcgacgcc attggccgcg gctccagtta catcaacagt gagtgcattc
    24541 gcgctaacct ggccaaagct ccagaggccg tgtgcattat cgccattggt tttaatggta
    24601 ctgtttgttc cgagatcgac aacagagttt ttctgtacgt ttatccccat ggcgctgtag
    24661 ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg cagcaccatt ggcgttattg
    24721 ccgttgagac caccgatata aacacctgta cttccatcgg tcgtgatttt acttccgctt
    24781 ccaagatcga cactggtgcc atagtcattg atggttaggc ctataccgtt cgagttttca
    24841 atggtgaatt gagtggctgt tagtgttgag ctatgcccaa taatgatgcc gtcatcgtta
    24901 ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat agtcaccaat taagttaatg
    24961 ccgatggcag aggtttgacc aacaacatct actgtgagtc ggttagctgt cagggagctg
    25021 tttttgccct gaataatgat tccttttgca tatccagagg cgtcgttgac attgacggtg
    25081 acatcattac ccaggttgat attgccaggc gtgtttcctg ctggggtcat tacaccatag
    25141 aggtttcctg atggatcggc ggtatctcct gtaattctgt caccatcatt aatagtaata
    25201 ttggtgccat attgacttcc tgtatcagca gctatcagca ggggggaaaa gataattgca
    25261 ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg gcgaacggat ttttaattca
    25321 gcgagacgat ctgctttggt attcccattg tgttgtttga tgcccatacc actcctatat
    25381 agtacccaga ctatgggtct atggattttg tcattttgtt taaatcaatg aataatatcc
    25441 tcttatcatc atgatatttg tatggtgcaa tcatgatgag cttctcagag aataattctc
    25501 tattaccggg caaaatcagg aacacaggaa ttaatatata tttaacatcc cccttacaaa
    25561 ggtgggttta tatcgagtta ttccctaagt tgtcgttgct tattccaatt cgttactata
    25621 aatatggcaa aaatattaca acaaacagcg gttaaaaaat ttggtgaaga ttttttgtaa
    25681 taatcctaat tagttatggg aatttcgctt gatgtatctg cataacaaaa acaatatgcg
    25741 ccatttttgc attactaata agaaaaagca aaaaacatcg atattcattt ttggtcaatg
    25801 ggttttctgg cctatcgttg ttattgtgct actggttatc attttcatct tgccaggatc
    25861 gcacgtaatc acgttattcg gggactttat ctgcgggata ttacccttca ggtaagtcct
    25921 tttctcgcca gcatattgga gcgtgatcaa tattgatcag ctgtgaaggg ggtgatgtgt
    25981 agtggaccaa aacttacgtt atatcactac taatgagata tccgtcatct gacactgatg
    26041 tctttaatta ttctcgtggt tcactgtagg caataagcac aaaagtgtag gatgttacaa
    26101 gaatgattag gactcggtga aatgaaaaat ccacgcaatt gcgtggatta tatatacttt
    26161 tgcgctcttc atgagattta gcgaaacctc atgagacaac aaattaatta gacgttgaaa
    26221 aggaagttca tcacatcgcc atctttaacg atgtaatctt taccttctgc acgcattttg
    26281 cctgcttctt tcgcgccttg ttcacctttg taagtgatga aatcttcaaa cgagatggtt
    26341 tgtgcacgga taaagccttt ttcaaaatca gtatggattt tgcccgctgc ctgcggcgcg
    26401 gttgctccaa ccggaatggt ccatgcacgc acttctttca ccccagcggt gaagtaagtt
    26461 tgcaggttca gcagcttata accggcacgg atcacacggt tcaggcccgg ctcttccagc
    26521 ccaagctcct gcataaactc gtcacgttct tcgtcgtcca gttcggcaat gtctgcttca
    26581 acagcagcac aaaccggaac cacaacagaa ccttctttcg ccgcgatttc acgcacctgg
    26641 tcaagatatg ggttgttttc aaaaccgtct tcgttgacgt tggcgatgta cattgttggt
    26701 ttcagcgtca ggaagctcag gtaacgaata gccgctttct cttcagcgct taaatccagc
    26761 gcgcgcagca tacctgcatt ttccaactgg ggcaggcatt tttccaggac cgccagctca
    26821 gctttcgcgt ctttatcgcc acctttggct ttcttctgta cgcgatgaat cgcacgttcg
    26881 caggtgtcga ggtctgccag cgccagttcg gtgttgataa cttcaatatc gtcagccggg
    26941 ttaactttgc cggaaacgtg aatgatgttg tcattttcaa agcagcgaac aacgtgaccg
    27001 atcgcttcgg tttcacggat gttggtcagg aactggttac ccagaccttc gcctttcgat
    27061 gcgcctttta ccagaccggc gatatcgaca aattccatgg tcgtgggaag cgtacgttgc
    27121 ggttttacga tttcagccag ttgatccagg cgagggtcag gcattggtac gacgcctgtg
    27181 ttcggctcaa tggtgcagaa tggaaagttg gccgcttcaa taccggcttt ggtcagcgcg
    27241 ttgaacaggg tagatttccc gacgttgggc aaaccgacga taccgcattt gaatcccatg
    27301 atttaaatca ccttaatatc ttaataatca acctgttatt gctaacagat tgcagaaatg
    27361 gaaataactt tgcctattat acacggcact cggcaaaaat gccgcagaca acgacttatt
    27421 gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta aaccacattt
    27481 cagtacaacg cgccgcttcg tcgatggctt catcaattaa cttctgttca ctaacaggcg
    27541 gtttgcctaa cacaaaaccg acaactttat ttttatcgcc cggatgaccg attccgatgc
    27601 gtaaacggtg aaagttaggg ttattaccca atttactgat gatgtctttc agtccattgt
    27661 gaccaccatg gccaccgccc aatttaaatt tggcgacgcc aggaggcaga tccagttcgt
    27721 cgtgggccac cagaatttcg tccggattaa tgcggaaaaa actggccatc gccgcaacgg
    27781 ctttgccgct gagattcata aatgtagtcg ggactaacag gcggacatct tcgcctccaa
    27841 gagtgactcg cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga gcgcgcaaac
    27901 gctctgccag taagtcaacg aaccaggcac cagcattatg tcgcgttgcg gcgtattcag
    27961 caccggggtt cgccaggccg acaatcaatt taatcgtcac gtttttttgt cctgagtgtg
    28021 tacataactg gcgcgtagtt tactggttgc ggccccgctt gacaaaaaac tgcgtatcaa
    28081 atgcagataa cgtaataatt gcatgagtgg actattagaa agtcaaggtg ttcaggcgtt
    28141 tatttgtaaa gttttgttga aataagggtt gtaattgtga tcacgcccgc acataaccca
    28201 cagggtgttg tctatacttt acacataagg aagaggggta ttccctgtta caacccagaa
    28261 agttccggag gtgacatatg aaacgcaaaa acgcttcgtt actcggtaac gtgctcatgg
    28321 ggttgggtct ggtggtaatg gtggtcggcg tggggtattc aatcctcaac cagttaccac
    28381 agtttaatat gccccagtat ttcgcacatg gtgcagtgct aagtattttc gtcggtgcca
    28441 ttctctggct ggcgggtgcc cgtgttggcg ggcatgaaca ggtgtgcgac cgttactggt
    28501 gggttcgcca ctatgacaaa cgttgccgcc gtagcgataa tcgccgtcat agctaacaaa
    28561 taatgcagtt tgctgaccag tcagtttgcg ctgactggtc aatctcgtac ttataaatcc
    28621 gccatcgccg cgcgacgatt cgggaagaac gccaggcgtc ccgggatcgg ttgaatgcca
    28681 gcgcgagcca tagtgcgcag tggctggaat tccacgttgc acacgcgcag ttcacatccc
    28741 tcgggcagac gcttcacaaa acgctggaac gcatcaagac caccagcatc aagtaccgga
    28801 accgcatccc acttcagaat cacaatccgt ttgccttcaa gacgtgactc caggtccgtg
    28861 aacaagcctt cagcagcagc aaaaaacagc gggccaataa cgcgcagaac caggacatcg
    28921 tctggaacat ctacgactac cggtgccagg cgagtcatac gtgcgatacg acgcataaac
    28981 agcagcgatg ccagcacgat ccccacgctg atggcaataa ccatatcaaa cagcacggtc
    29041 agcgacatgc acagcagcat gacaatgatg tcatctttcg gcgcatgacg cagcaagtcg
    29101 accactttgt gcgcttcact catgttccac gccaccatca acagcagggc agccatggcg
    29161 gaaagcggca gccaggagag cagcggtgcc agtaccagca gggcaagaat aaccagaata
    29221 gagtggatca ccgccgagat aggggaagtt gccccggcac ggacgttagc ggcagaacgc
    29281 gcgatggcag ctgtagcggt aatgccacca aagaacggag cgatgatatt ccccagtccc
    29341 tgtccaacca gttcgctatt cgccttgtgt ttcgtcccgg tcataccatc cagcaccacg
    29401 gcgcagagca gagattcgat tgcgccgagc attgccattg agaatgccgc aggcagcagt
    29461 gtgcgaatag aatcccaggt tagcgtgaat tctgaattag gcagatccca cggcagcacc
    29521 agttgcggca gcagttgcgg aataccgtta ccctgagaac catcggccag gacgtagtgg
    29581 aattgcgaac cgatggtagc aacatgtccg ccgagcaggt taacaatccc catcaccgcg
    29641 caaccagcca gcaaggccgg aaggtgaccg ggtaaacgaa tgcccagacg cggccaaaaa
    29701 acaagaatac ctagcgtcac aatgccaatg gcagcatcac ccacattaat ggtcggcagc
    29761 gccataaata atgcgccgac tttttgtaga taatgttccg ggacatgggc catttgcaga
    29821 ccgagaaaat ctttaatctg catggtaccg atggtgatcc cgatacccga ggtgaaacct
    29881 aaggtgacgg aaaccggaat atactcaatc aggcgaccaa agcgtgccag acccatcaga
    29941 atcaaaaaaa tccccgacag caaggtcgca accagcagtc ctgccagtcc aaactgttgc
    30001 gacacgggat agagaattac cacaaatgcc gcagtcggac cggaaacgct aaagcgtgac
    30061 ccacccgtca gagcaatgac aatccccgca acagctgcgg tatataaacc gtactggggt
    30121 gccacaccac taccaatagc caacgccatc gccagcggga tagcaataat cccgacggtt
    30181 atcccggcaa tcaggtcacg ggtaaaccgt gcggcagtat atttttcttt ccagcaagcg
    30241 tcgatcagag cgcggaaagg catcacatgt gaggaaaata ttttgttcac aataatgttt
    30301 catccgtgag cgcatcatct gtcaactaaa tggcaggtga aggaggcata ggtcatacaa
    30361 atggatatta cagacaaaaa aacccgccgc agcgggtctt tgagccgggt tcgattagtg
    30421 ttcgaacatg gcagagatcg attcttcgtt gctgatacga cgaatcgctt cggccagcat
    30481 acctgacagg gtcagagtac gcacgttcgg cagtgatttg atttcatcgc tcagcggaat
    30541 ggtatcgcag acaacgactt catcaattac agagttacgc aggttattcg ccgcgttgcc
    30601 agagaagatc gggtgagtcg cgtacgcaaa tacacgttta gcaccacgtt ctttcagagc
    30661 ttcagcagct ttacacagcg taccgccagt gtcgatcata tcatcgacca gtacgcagtc
    30721 acgacctgca acgtcaccga tgatatgcat cacctgggaa acgttcgcac gcggacgacg
    30781 tttgtcgatg attgccatat cggtatcgtt cagcagctta gcgatagcgc gggcacgcac
    30841 aacgccgccg atgtccggag aaaccacaat tgggttatcc agattcagct gcagcatgtc
    30901 ttccagcagg atcgggctac caaatacgtt atcaaccgga acgtcgaaga aaccctgaat
    30961 ctgttcagcg tgcagatcca ctgtcagcac acggtcaaca ccgacgctgg agaggaagtc
    31021 tgcaaccact ttcgcagtga ttggtacacg agcggaacgg acgcgacggt cctggcgcgc
    31081 atagccaaag taggggataa cagcggtgat acgacctgcg gaagcacgac gcagggcatc
    31141 aaccataacg actaattcca tcaggttgtc gttagtaggg gcacaagtgg actggatgat
    31201 gaaaatatca ccaccgcgta cattttcgtt gatttgtaca ctgacttcgc cgtcgctaaa
    31261 gcgacctaca gcggcgtcgc cgagtgaagt gtacaggcgg ttggcaatac gttgtgctag
    31321 ttccggggtg gcgttaccag caaaaagctt catatcaggc acgagaagaa cctcaggcat
    31381 gcgtccattg gtggaaagaa tctgccgaaa actgtgcggg ccaggcatga tcctttccag
    31441 gcggtgtatt aaagagcgcg atgcaacgtc tggaacaagg tgacgttgtc accgaaactc
    31501 agcttgcccg gcttaaagca tggctctgtg caatggggaa agattaacgc ctttcgccac
    31561 aaagccattg agccattccg gggcttgctc tagcacctgg cgggcttcag actctgtatc
    31621 aaattcagca aagacacagg cccctgtccc agtcaggcgc gacggggcgt attctaacag
    31681 ccaggaaagc accgcatcaa cctcgcgaaa acgttttctt gcgataacct cgcaatcatt
    31741 gctgaattca cattttagca acgtttctat tgaccttttt ggcgtattgc gcgggagttc
    31801 aggatcttta aaaatcaccg gagtcggaat acttacacca gggtgcgcca ccagatacca
    31861 cttctctggc ggatccaccg gcgttagtat ttcaccaacg ccttcggcaa acgcggcatg
    31921 cccccgaaca aagacaggaa catctgcgcc cagcgtcagc cccatttccg ccagctcatc
    31981 catgcttagc ccgcattgcc agagatgatt taatgccacc aggaccgtcg cggcattgga
    32041 tgaaccaccg ccgagaccgc cgcccatcgg caaacgcttg tcaatgctga tattcgcacc
    32101 gcttcccgtc ggaagacgcc cgctgtctgc cgcagttttt atcaacaatc gcgctgcgcg
    32161 aacgatcagg ttatcttcat gttccacgcc ttcaacgggc gttaacagac gaatatcccc
    32221 atcgtcacga agctcaatgc tgatggtgtc gccgtaatca agaaactgaa acagcgtttg
    32281 cagcgtgtgg taaccatccg cacgctgacc ggtaatgtat aaaaacagat taagttttgc
    32341 cggagagggc cactgtgtcc gcattatttc actatccagt tatccatttt taacttgatg
    32401 cgttgaccac cgtcggtgag ttccatattg gctggcatcg caggttgcgt tttggtgtca
    32461 taaccaccat aaacaacctt ccagtttttg ccattctggc tgtaggtaat ttcgctcagg
    32521 cggtactggt cgtccagttt gtagtcggtt gcatcacccg gtaaacctaa aatccactgg
    32581 cgcaagctgt tgagcggaat tggcattccg gtcaatttgc caatcatctc ttcggcgtca
    32641 tcggcggtat aacgctgacc tttattgtcg actaactgca cgttacccgg ttgagcattc
    32701 agctccagtt ccgtgctgcc caatgggtta gtgagcagca gacggtagcg atcctggccg
    32761 gtttgctgcc agaaaaagcg ggcgtacact ttttgttggt cagaaatata agcgaacgcg
    32821 ccgcgagtct gatactgatt aagattgcgc acgtcttgct gatgctgacg ccattgtggc
    32881 gaatccgggc ttttgccagg acctttgggc gtggtaacgg aacaggcagt gagcacaaga
    32941 gcagccagcg gtagcaggcg gataagacga aaatcgggca ggggcatagt gatgacaagt
    33001 ccttgagata cgttgcagtt ataaccctta atgctagcgt taccgtccgc tatcgtctat
    33061 gttcaagttg tcttaattgc cagaatctaa cggctttcgg caattactcc aaaagggggc
    33121 gctctctttt attgatctta cgcatcctgt atgatgcaag cagactaacc ctatcaacgt
    33181 tggtattatt tcccgcagac atgacccttt tagcactcgg tatcaaccat aaaacggcac
    33241 ctgtatcgct gcgagaacgt gtatcgtttt cgccggataa gctcgatcag gcgcttgaca
    33301 gcctgcttgc gcagccgatg gtgcagggcg gcgtggtgct gtcgacgtgc aaccgcacgg
    33361 aactttatct tagcgttgaa gagcaggata acctgcaaga ggcgttaatc cgctggcttt
    33421 gcgattatca caatcttaat gaagaagatc tgcgtaaaag cctctactgg catcaggata
    33481 acgacgcggt tagccattta atgcgtgttg ccagcggcct ggattcactg gttcttgggg
    33541 agccgcagat cctcggtcag gttaaaaaag cgtttgccga ttcgcaaaaa ggtcatatga
    33601 aggccagcga actggaacgc atgttccaga aatctttctc tgtcgcgaaa cgcgttcgca
    33661 ctgaaacaga tatcggtgcc agtgctgtgt ctgtcgcttt tgcggcttgt acgctggcgc
    33721 ggcagatctt tgaatcgctc tctacggtca cagtgttgct ggtaggcgcg ggcgaaacca
    33781 tcgagctggt agcgcgtcat ctgcgcgaac ataaagtaca gaagatgatt atcgccaacc
    33841 gcactcgcga acgtgcccaa attctggcag atgaagttgg cgcggaagtg attgccctga
    33901 gtgagatcga cgaacgtctg cgcgaagccg atatcatcat cagttccacc gccagcccgt
    33961 taccgattat cgggaaaggc atggtggagc gtgcattaaa aagccgtcgc aaccaaccaa
    34021 tgctgttggt ggatattgcc gttccgcgcg atgttgagcc ggaagttggc aaactggcga
    34081 atgcttatct ttatagtgtg gacgatctgc aaagcatcat ttcgcacaac ctggcgcagc
    34141 gtaaagccgc agcggttgag gcggaaacta ttgtcgctca ggaaaccagc gaatttatgg
    34201 cgtggctgcg agcacaaagc gccagcgaaa ccattcgcga gtatcgcagc caggcagagc
    34261 acgttcgcga tgagttaacc gccaaagcgt tagcggccct tgagcagggc ggcgacgcgc
    34321 aagccattat gcaggatctg gcatggaaac tgactaaccg cttgatccat gcgccaacga
    34381 aatcacttca acaggccgcc cgtgacgggg ataacgaacg cctgaatatt ctgcgcgaca
    34441 gcctcgggct ggagtagcag tacatcattt tcttttttta cagggtgcat ttacgcctat
    34501 gaagccttct atcgttgcca aactggaagc cctgcatgaa cgccatgaag aagttcaggc
    34561 gttgctgggt gacgcgcaaa ctatcgccga ccaggaacgt tttcgcgcat tatcacgcga
    34621 atatgcgcag ttaagtgatg tttcgcgctg ttttaccgac tggcaacagg ttcaggaaga
    34681 tatcgaaacc gcacagatga tgctcgatga tcctgaaatg cgtgagatgg cgcaggatga
    34741 actgcgcgaa gctaaagaaa aaagcgagca actggaacag caattacagg ttctgttact
    34801 gccaaaagat cctgatgacg aacgtaacgc cttcctcgaa gtccgagccg gaaccggcgg
    34861 cgacgaagcg gcgctgttcg caggcgatct gttccgtatg tacagccgtt acgccgaagc
    34921 ccgccgctgg cgggtagaaa tcatgagcgc cagcgagggt gaacatggtg gttataaaga
    34981 gatcatcgcc aaaattagcg gtgatggtgt gtatggtcgt ctgaaatttg aatctggcgg
    35041 tcatcgcgtg cagcgtgttc ctgctacgga atcgcagggt cgtattcata cttctgcttg
    35101 taccgttgcg gtaatgccag aactgcctga cgcagaactg ccggacatca acccagcaga
    35161 tttacgcatt gatactttcc gctcgtcagg ggcgggtggt cagcacgtta acaccaccga
    35221 ttcggcaatt cgtattactc acttgccgac cgggattgtt gttgaatgtc aggatgaacg
    35281 ttcacaacat aaaaacaaag cgaaggcgct ttcagtgctc ggtgctcgca tccacgctgc
    35341 tgaaatggca aaacgccaac aggccgaagc gtctacccgt cgtaacctgc tggggagtgg
    35401 cgatcgcagc gaccgtaacc gtacttacaa tttcccgcag gggcgcgtta ccgatcaccg
    35461 catcaacctg acgctctacc gcctggatga agtgatggaa ggtaagctgg atatgctgat
    35521 tgaaccgatt atccaggaac atcaggccga ccaactggcg gcgttgtccg agcaggaata
    35581 atggaatatc aacactggtt acgtgaagca ataagccaac ttcaggcgag cgaaagcccg
    35641 cggcgtgatg ctgaaatcct gctggagcat gttaccggca gagggcgtac ttttattctc
    35701 gcctttggtg aaacgcatct gactgacgaa caatgtcagc aacttgatgc gctactgaca
    35761 cgtcgtcgcg atggtgaacc cattgctcat ttaaccgggg tgcgagaatt ctggtcgttg
    35821 ccgttatttg tttcgccagc gaccttaatt ccgcgcccgg atacggagtg tctggtggag
    35881 caggcactgg cgcggttgcc tgaacaacct tgccgtattc ttgatctcgg gacgggtacc
    35941 ggggcgattg cgcttgcgct ggctagcgag cgcccggact gcgaaattat cgctgtagat
    36001 cgtatgcctg atgctgtctc cctggcacaa cgtaatgccc agcatctggc gatcaaaaat
    36061 atccacattc tgcaaagcga ctggtttagc gcgctagccg ggcagcagtt tgcgatgatt
    36121 gtcagcaatc cgccgtatat tgacgagcag gacccacatc ttcaacaagg cgatgtccgc
    36181 tttgagccgc tcactgcgct ggttgcggca gacagtggaa tggcagacat cgtgcatatc
    36241 atcgaacagt cgcgtaacgc gctggtatcc ggcggctttc tgcttctgga acatggctgg
    36301 cagcagggcg aagcggtgcg acaagcattt atcctcgcgg ggtatcatga cgtcgaaacc
    36361 tgccgtgact atggtgataa cgagcgcgta acgctcggcc gctattatca atgacaagtt
    36421 tttctacact gcttagtgtt catcttatta gtctcgcgct ttctgttggg ctattaacct
    36481 tacgtttctg gctacgttat cagaagcatc cacaggcatt tgctcgctgg acgcgcattg
    36541 tgccgccggt tgtcgatacg gtgttattgt taagcggcat tgcgttgatg gctaaagcgc
    36601 acatcctgcc attttccggg caggcacagt ggctgactga aaagctgttt ggagttatca
    36661 tttatatcgt tttgggtttt attgcactcg attatcgtcg tatgcacagt cagcaggcgc
    36721 gcattattgc cttcccgctg gcgttggtgg tgctgtacat catcattaaa ctcgccacca
    36781 caaaagtacc gttactgggg taagtcatga gatcgttagc tgatttcgaa tttaataaag
    36841 cgccattgtg cgaaggcatg atcctggctt gcgaagcaat ccgccgcgat tttccctcgc
    36901 aagatgttta cgacgaactg gagcgtctcg ttagtctggc gaaggaagaa atcagccagc
    36961 ttctgccttt agaagagcag ctggaaaaac tgatcgcgct gttttacggc gactggggat
    37021 ttaaagcctc acgcggcgtt tatcgtcttt ccgatgcatt atggctggac caggtgttaa
    37081 agaatcgaca gggcagtgcg gtatcattag gtgcggtttt attatgggtc gcgaatcgtc
    37141 tcgatttgcc gctgctgccg gtgattttcc ctacgcagct gatattgcgc attgaatgtc
    37201 cggatggcga aatttggctg attaatcctt ttaacggtga atcgttaagc gaacatatgc
    37261 tggacgtatg gttaaaggga aatatcagcc cgtcggcgga actgttttat gaagaccttg
    37321 atgaagctga taacattgag gtaatccgca aattgctgga tacactcaaa gcctcgttga
    37381 tggaagaaaa tcagatggag ctggcgttac gcaccagcga agctttatta caattcaacc
    37441 ctgaagatcc ctatgaaatt cgcgatcgcg ggttgattta tgcgcaactg gattgcgaac
    37501 acgttgcgtt gaacgattta agttatttcg ttgaacagtg tccggaagac ccgatcagcg
    37561 aaatgatccg tgcgcaaata aataacatcg cgcataaaca tattgtgctg cattaattaa
    37621 tcgacatttt actcaagatt aaggcgatcc tatgaaacaa aaagtggtta gcattggcga
    37681 catcaacgta gcaaatgacc tgccgttcgt actgtttggc ggtatgaacg tgctggaatc
    37741 tcgcgatctg gcgatgcgca tttgcgagca ctacgtaact gtgacccaga aactgggtat
    37801 cccttacgtg ttcaaagcct cttttgacaa agccaaccgc tcctccatcc actcttatcg
    37861 tggaccgggc ctggaagaag ggatgaaaat cttccaggag ttgaagcaga cttttggcgt
    37921 gaaaattatc accgacgttc acgaaccaag tcaggcacag cccgttgctg atgtcgtgga
    37981 tgtgattcag ttgccggcgt ttcttgctcg ccagactgac ctggttgaag ccatggcgaa
    38041 aaccggtgcg gtaattaacg tcaagaaacc acagtttgtc agcccgggac agatgggtaa
    38101 tatcgttgat aaattcaaag aaggcggcaa cgaaaaagtg attctttgcg atcgtggtgc
    38161 taacttcggc tatgacaacc tggttgtcga tatgctgggc ttcagcatta tgaagaaagt
    38221 gtctggtaac tcgccggtga ttttcgacgt gacccacgca ctgcaatgcc gcgatccgtt
    38281 tggcgcagct tccggtggtc gtcgtgctca ggtggctgag ctggcacgag ccggtatggc
    38341 ggtaggtctg gcggggctgt ttattgaagc gcatccggat ccggaacatg cgaaatgtga
    38401 tggtccatcc gcgctgccgc tggctaaact ggaaccgttc ctcaagcaga tgaaagcgat
    38461 tgatgatctg gtgaaaggtt tcgaagaact ggataccagc aagtaatctt ttttgcttga
    38521 aaaataaagt cttagcgttc tgcgttaaga cttttttcat gggtgccaga tacaaaaaag
    38581 gccgcaggct gttacccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga
    38641 cgcccgtttt caccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt
    38701 cccgccagga tcggtgctgc caggtcgtgc cagaaaatca tggcaaactg cgcgagcgtc
    38761 atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaattg ttctggttca
    38821 agattagccc ccgttctgtt gtcaggttaa acctctcaac gtgcgggggt tttctctttc
    38881 cagcaaccaa tgccaccagg gataaagccc ccgcaacatt gcgcctcacc ggataacgcc
    38941 ggcttgatgt ggatactacg tctcaattca tcttcacttc atccctgaaa tgtttgcaat
    39001 gaagagtgca ttccggtttt tcaacagctg ttacagtcgt ttcatgaggg ctctggatga
    39061 tgcttccagc tcgggttgcc aatatttact tgtggaagag ataaagacaa aaatggccgc
    39121 aggctgttac ccctgcggcc ggtttcgggc gcatattgcc atcacggcag cctgacgccc
    39181 gttttcacct tacttccggt tacgccacca gccgacaatc gctgcggtaa taatccccgc
    39241 caggatcggt gctgccaggt cgtgccagaa aatcatggca aacttcgcga gcgtcatata
    39301 gccgccttgt tgtaatgaca atattttgcg gctattcttg a
//
LOCUS       DAZFWP020000026        39011 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFWP020000026 DAZFWP020000000
VERSION     DAZFWP020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.026"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(196..387)
                     /gene="yfgG"
                     /locus_tag="ACHK0Z_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             complement(196..387)
                     /gene="yfgG"
                     /locus_tag="ACHK0Z_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGU4501286.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            405..557
                     /locus_tag="ACHK0Z_RS06160"
                     /old_locus_tag="pgaptmp_001232"
     CDS             405..557
                     /locus_tag="ACHK0Z_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501287.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            739..2982
                     /gene="pdeF"
                     /locus_tag="ACHK0Z_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             739..2982
                     /gene="pdeF"
                     /locus_tag="ACHK0Z_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU4501288.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(3021..4562)
                     /gene="ppx"
                     /locus_tag="ACHK0Z_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             complement(3021..4562)
                     /gene="ppx"
                     /locus_tag="ACHK0Z_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4501289.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4567..6633)
                     /gene="ppk1"
                     /locus_tag="ACHK0Z_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             complement(4567..6633)
                     /gene="ppk1"
                     /locus_tag="ACHK0Z_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU4501290.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6804..7442)
                     /gene="purN"
                     /locus_tag="ACHK0Z_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             complement(6804..7442)
                     /gene="purN"
                     /locus_tag="ACHK0Z_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU4501291.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7442..8479)
                     /gene="purM"
                     /locus_tag="ACHK0Z_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             complement(7442..8479)
                     /gene="purM"
                     /locus_tag="ACHK0Z_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU4501292.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8804..9430
                     /gene="upp"
                     /locus_tag="ACHK0Z_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             8804..9430
                     /gene="upp"
                     /locus_tag="ACHK0Z_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU4501293.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9516..10805
                     /gene="uraA"
                     /locus_tag="ACHK0Z_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             9516..10805
                     /gene="uraA"
                     /locus_tag="ACHK0Z_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU4501294.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10900..11601
                     /gene="hda"
                     /locus_tag="ACHK0Z_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             10900..11601
                     /gene="hda"
                     /locus_tag="ACHK0Z_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU4501295.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11739..12098)
                     /gene="arsC"
                     /locus_tag="ACHK0Z_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             complement(11739..12098)
                     /gene="arsC"
                     /locus_tag="ACHK0Z_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU4501296.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12119..13582)
                     /gene="bepA"
                     /locus_tag="ACHK0Z_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             complement(12119..13582)
                     /gene="bepA"
                     /locus_tag="ACHK0Z_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU4501297.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13795..14856
                     /gene="yfgO"
                     /locus_tag="ACHK0Z_RS06215"
                     /old_locus_tag="pgaptmp_001243"
     CDS             13795..14856
                     /gene="yfgO"
                     /locus_tag="ACHK0Z_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4501298.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14894..15745)
                     /gene="focB"
                     /locus_tag="ACHK0Z_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             complement(14894..15745)
                     /gene="focB"
                     /locus_tag="ACHK0Z_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU4501299.1"
                     /translation="MHEKQTLFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGV
                     FMAIGFTFYLSVIADTPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSR
                     GVISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHT
                     FTESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIP
                     FAIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRA
                     IYLRQES"
     gene            complement(15763..17775)
                     /gene="hyfR"
                     /locus_tag="ACHK0Z_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             complement(15763..17775)
                     /gene="hyfR"
                     /locus_tag="ACHK0Z_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU4501300.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHFDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRSMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17805..18218)
                     /gene="hyfJ"
                     /locus_tag="ACHK0Z_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             complement(17805..18218)
                     /gene="hyfJ"
                     /locus_tag="ACHK0Z_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU4501301.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18211..18968)
                     /gene="hyfI"
                     /locus_tag="ACHK0Z_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /pseudo
     CDS             complement(18211..18968)
                     /gene="hyfI"
                     /locus_tag="ACHK0Z_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18965..19503)
                     /gene="hyfH"
                     /locus_tag="ACHK0Z_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /pseudo
     CDS             complement(18965..19503)
                     /gene="hyfH"
                     /locus_tag="ACHK0Z_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
     gene            complement(19513..21180)
                     /gene="hyfG"
                     /locus_tag="ACHK0Z_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(19513..21180)
                     /gene="hyfG"
                     /locus_tag="ACHK0Z_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU4501302.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21170..22756)
                     /gene="hyfF"
                     /locus_tag="ACHK0Z_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             complement(21170..22756)
                     /gene="hyfF"
                     /locus_tag="ACHK0Z_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU4501303.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(22761..23411)
                     /gene="hyfE"
                     /locus_tag="ACHK0Z_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             complement(22761..23411)
                     /gene="hyfE"
                     /locus_tag="ACHK0Z_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU4501304.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23423..24653)
                     /locus_tag="ACHK0Z_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /pseudo
     CDS             complement(23423..24653)
                     /locus_tag="ACHK0Z_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(24670..25617)
                     /gene="hyfC"
                     /locus_tag="ACHK0Z_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             complement(24670..25617)
                     /gene="hyfC"
                     /locus_tag="ACHK0Z_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU4501305.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25628..27646)
                     /gene="hyfB"
                     /locus_tag="ACHK0Z_RS06270"
                     /old_locus_tag="pgaptmp_001254"
     CDS             complement(25628..27646)
                     /gene="hyfB"
                     /locus_tag="ACHK0Z_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGU4501306.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAIPALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQSLRVVFSALFRLRQQFDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27646..28263)
                     /gene="hyfA"
                     /locus_tag="ACHK0Z_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             complement(27646..28263)
                     /gene="hyfA"
                     /locus_tag="ACHK0Z_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU4501307.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28516..28986)
                     /gene="bcp"
                     /locus_tag="ACHK0Z_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             complement(28516..28986)
                     /gene="bcp"
                     /locus_tag="ACHK0Z_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU4501308.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28986..29558)
                     /gene="gcvR"
                     /locus_tag="ACHK0Z_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             complement(28986..29558)
                     /gene="gcvR"
                     /locus_tag="ACHK0Z_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU4501309.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29704..30582
                     /gene="dapA"
                     /locus_tag="ACHK0Z_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             29704..30582
                     /gene="dapA"
                     /locus_tag="ACHK0Z_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU4501310.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30599..31633
                     /gene="bamC"
                     /locus_tag="ACHK0Z_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             30599..31633
                     /gene="bamC"
                     /locus_tag="ACHK0Z_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU4501311.1"
                     /translation="MAYSVQKSRLAKIAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31846..32559
                     /gene="purC"
                     /locus_tag="ACHK0Z_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             31846..32559
                     /gene="purC"
                     /locus_tag="ACHK0Z_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU4501312.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32714..32941
                     /gene="vapB"
                     /locus_tag="ACHK0Z_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             32714..32941
                     /gene="vapB"
                     /locus_tag="ACHK0Z_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4501313.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32941..33339
                     /locus_tag="ACHK0Z_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             32941..33339
                     /locus_tag="ACHK0Z_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4501314.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33486..34349
                     /gene="ypfJ"
                     /locus_tag="ACHK0Z_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             33486..34349
                     /gene="ypfJ"
                     /locus_tag="ACHK0Z_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU4501315.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34364..36379
                     /gene="tmcA"
                     /locus_tag="ACHK0Z_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             34364..36379
                     /gene="tmcA"
                     /locus_tag="ACHK0Z_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU4501316.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36453..37151
                     /gene="ypfH"
                     /locus_tag="ACHK0Z_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             36453..37151
                     /gene="ypfH"
                     /locus_tag="ACHK0Z_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4501317.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37261..37461)
                     /gene="ypfN"
                     /locus_tag="ACHK0Z_RS06330"
                     /old_locus_tag="pgaptmp_001266"
     CDS             complement(37261..37461)
                     /gene="ypfN"
                     /locus_tag="ACHK0Z_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU4501318.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37489..38616)
                     /gene="dapE"
                     /locus_tag="ACHK0Z_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             complement(37489..38616)
                     /gene="dapE"
                     /locus_tag="ACHK0Z_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU4501319.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38620..38976)
                     /gene="yffB"
                     /locus_tag="ACHK0Z_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             complement(38620..38976)
                     /gene="yffB"
                     /locus_tag="ACHK0Z_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU4501320.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9536 a         9992 c        10237 g         9246 t
ORIGIN      
        1 aatttataaa aatatatcat tacaacatat atattaattt tatatttatt tcgcagccaa
       61 acaggataaa catcagtcag gacaaagacg taaaatttaa gatgaaagaa tatctgccgc
      121 tttgataatc ccaaagagca aaagcatccg acagcgaagt gccgttaggt tctgtgaaag
      181 tggagaaggt aaccgctaac gttgtaccgg tgattcgacg gagagtgtgg attgctgagc
      241 ttcttttttg ctctgatggt gctgccaggc accgacggac gagtagataa aacggccaaa
      301 gaagaagata aagctgatga gcagtacgat acgggtcatg cgactgttaa atcggtgtcg
      361 ttttcgcata ctggttgcct gactcacaaa aggttccttg aagtatgtcc cacgctgtgg
      421 acggtactta cattaaggca caacaggaca aaatggtcaa ttcttcgtta tgtaaaaaag
      481 cgtcagtgga tacatatttt aatgttatgg aagttaattt aattatttac aatgatgatg
      541 taataatgat gaattaacat gataaacgta ataattcatt aatcagggtt attttgtttg
      601 atatatatca attggaactt tcatatgaca cttagaatca atgctctcta tagtgatgaa
      661 taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc aggatgtggg
      721 ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg tgataaatgg
      781 tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca cattaatact
      841 ttcgcgcata tttcttccgg tgaggttttt ctcttttatc tgcctttggc actgatgatc
      901 agcatgatga tgtttttcag ctgggcggca ttgccaggga tcaccttagg gatttttgtc
      961 cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa ttttattatc
     1021 attatcattc tctgttgggg cggttacagg gtctttactc cccggcgtaa caacgtttca
     1081 catggtgata cccgtttaat ttcccagcgt atattctggc agatagtgtt tcctgcaacg
     1141 ctgtttctga tacttttcca gtttgctgca tttgtaggat tactggcgag cagagaaaat
     1201 ctggtcggtg tcatgccttt taacctcggg actttaatca attatcaggc cttactggtg
     1261 ggtaatctga tcggtgtccc gctgtgctac ttcatcattc gggtagtgcg aaatccattt
     1321 tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaagt caccaaaaaa
     1381 gagttcgcgc tctggctact ggcattaggt gctttattgt tgctgttatg catgccgtta
     1441 aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgtt gcccctgatg
     1501 atgtggggag caatgcgcta tggttataag ctgatttcgc tgctctgggc ggtcgtgttg
     1561 atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta taccacgcag
     1621 ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta tatggctgta
     1681 ctggcaactc gtcagcgagc ggtagtcaga cgcattcagc ggcttgcgta tgtggacccg
     1741 gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct
     1801 gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaacta tggcatcatg
     1861 ctgcggattc aatacaagca aaaactttct cactggctgt cacccttgct ggaaccgggt
     1921 gaagatgttt atcagctttc gggtaacgat ctcgcgctgc ggctgaatac agaatcgcac
     1981 caggagcgca ttaccgcact ggatagccat ctcaagcaat ttcgtttctt ttgggatggc
     2041 atgccgatgc aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc agtgaatcat
     2101 atctacctgc tgctgggaga gctaaatacg gtcgccgaac tttccatcgt gaccaacgcc
     2161 ccggaaaata tgcagcgtcg cggggcaatg tatttgcaac gcgaattgaa agataaagtc
     2221 gcgatgatga atcgactaca gcaggcgctg gaacacaacc attttttcct gatggcccag
     2281 ccgattaccg gtatgcgtgg tgatgtttac catgaaattc ttctgcgcat gaaaggtgag
     2341 aatgatgaac tgatcggccc cgatagcttc ttgccggtcg cgcacgaatt tggtttatcg
     2401 tcgagtatcg acatgtgggt cattgagcat acgctgcaat ttatggctga aaacagagcg
     2461 aagatgcccg ctcaccgttt tgctattaat ctgtctccaa cctcggtatg tcaggctcgt
     2521 tttcctgttg aagtcagtca gttgctggct aaatatcaga ttgaagcgtg gcaacttatt
     2581 tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc aggcgcaaat caccttgcag
     2641 catcttcagg aattaggctg ccagattgcg attgatgatt tcggcaccgg ctacgccagc
     2701 tatgcacggc ttaaaaatgt gaatgccgat ctgcttaaaa ttgacggcag ttttatccgc
     2761 aatattgtgt caaatagtct ggattatcag atagtggcat cgatttgcca cctggcgcga
     2821 atgaagaaaa tgcgggtagt ggcagagtac gttgaaaacg aagagatccg cgaggcggtg
     2881 ctctctttgg ggatcgatta tatgcagggt tatcttattg gtaagccgca gccgttaatt
     2941 gatacgttga atgaaatcga acccattcgc gaaagtgcct gaataatgcg ggccgacatt
     3001 tctcgtcggc ccgcaaagca ttaagcggcg atttcaggtg tactttcttc ttcaattttc
     3061 aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc aagcagtacc
     3121 agcgcattct gactaaacca gtcatgcggg aaacgcagtg tccagtgact gtcatcagta
     3181 atcagtgtca atgttggcgg tgtggtggtt gcctgacgtt gattgtttag taatacgcca
     3241 aggcgcaata gctgtatcag tggcaggaac tgtttcttct taaacaaggt aaagcgcggc
     3301 agatcgtcga gcttaatcgc tttacggtga tagcgcacca gtgtcgccat catcagctgc
     3361 tgttcctgat taaaacccgg caagtcactg ttttgcagaa tataagcgga gtggcgatgc
     3421 aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca tcgcagtagc
     3481 gcctccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc gtacatttgc
     3541 atagtggtat ccagcactcg tcgggcctgt tcgctgtcga tgtgatactg gttggcgagg
     3601 ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat ttcatacagt
     3661 acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa agcatcaaac
     3721 acaccgcata aaatcgccag tccaggaacg aagactgttt tccgctcttc ggaaagaccc
     3781 ggtaaactca gcgatgcgaa attacggtga cgtaaaactt cttttaccag tttttccaga
     3841 cgttccgggg taattatccc gtctttctcg cccatttcca ttaacacttc atgggcggct
     3901 tttatggtac cggaagcgcc cattgccacg ttccagccct gaatacggaa ttgccaggtt
     3961 aaagtttcca gtttttgtgc tgccgccata cgagcgcgct gaaaattctc tttattgatg
     4021 accccgccag ggaagtaaag ctgggcaaag ctgacacaac ccatccggcg gctttcaacg
     4081 agaatcggtt cgaaattttc accaatcacc agttccgtag atccgccgcc aatatcaata
     4141 accagtttgc gacctttctc cggttgggta tgttccacgc ccataaaaat cagacgggct
     4201 tcttcattac cggaaataat ttcaatcggg tagggaatga ccttttccgc gcgtttcaga
     4261 aagtcagtgg cgttcagcgc ctgacgcagc gtatgggtgc cgactataca gacgctggca
     4321 ggagaaaacc cttgtagccg ttcggcaaac agcgacagac agttcaaacc gcgcgtcatt
     4381 gcctcttcac tcaacatatt atctggcccc aggccgtccg ccagatgcac ccgctgtttc
     4441 aggcggccaa taatctgcat ggcaccatct accacacggg ctatgaccat gtgaaaactg
     4501 tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag gggatttatc gtgtattggc
     4561 atagggttat tcaggttgtt cgagtgattt gatgtagtcg taaatcgcca actgcgcccg
     4621 tactttgcgg cgattgccgc ggggaacgta gcgattactg agttctttat cgatataacg
     4681 tgctttgacc gtatcgctga acaatatgtc gatgatgtcc agcacccgct gcttcaggcg
     4741 cggatcgagc agcggtgtcg ccacttcaat acgataatca atattgcgcg tcatccagtc
     4801 ggcggaagaa aggtagacct ttttatcgcc gccattttca aaaatataaa cccggtcatg
     4861 ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg tcgctaatgc cttccagatt
     4921 ggggatcagc gaacacattc cgcgaaccag cagattaacc ggtacgccgg agctggaggc
     4981 cgcatacaga cgatcaacca ggcctttatc gacaaggtta tttagcttca gggtgatacc
     5041 actgggcagc ccttgctgcg cgttggcgat ttcgcggtct accatttcat ataacagacg
     5101 gcgagagttt tgcggtgaca ccattaaata atcaaatgtc accgggcggt atgggttttc
     5161 aataaagtta aatacccgcc gtacttcgtt ggtgatacgc gcatctgcgg tcagcaacga
     5221 atagtcagta taaagacgcg cggttttttc gttaaagttc ccggtcccga tgtgtgcgta
     5281 acggaccact tcaccgtttt ctttacgtga aatcaggaac agtttggcgt gaattttcag
     5341 ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg cccagtgaat
     5401 gttggcttct tcgtcgaaac gcgcctgtaa ctcaaccacc acggtgactt tcttaccgtt
     5461 atgtgcggcg tggatcatcg agtcgatgat gcgtgaatct ttcgccacgc ggtaaatgtt
     5521 aattttaatc gccagtacgc tcgggtcgaa cgaagcctga cgcagcagtt ccagcacatg
     5581 ctcaaaggtg tgataaggat aatagagcaa cacatcgcgt tcgcgaatgg catcaaaacc
     5641 attgcggaac tgggctttat caaaccaaat gtggcgtaaa cgcggcagtg gtttgttcac
     5701 cagattggct ttgccgacat tggggaaatt aataaagtct ttaaaattat gataacgacc
     5761 gccggggacg atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca cttcaaccag
     5821 cgcattgggc atatcgcgct gataaacaaa acgcaccggc tcagcagtta aacgctgctt
     5881 gagactggaa gacattaact ccatcaggct ggcttccatc tcatgcacta aatcgtattc
     5941 ggcatcgcgg gtcatcttca ttgaataggc attcagcgcg tcataatcaa agaagccttt
     6001 gaaaatatca tcgaggcagt aacgcagaat gttatccaaa agaatcatcg gcttgcgtcg
     6061 acgcggtgct tctggcggta aattcacaaa gcgcggcact ttatctgatg ggatctccag
     6121 cagcgcgtaa cggatggtat cgccacggat aatttccacc gccagatagg tgtaatcatc
     6181 tttcaggaac tgcactaagt cagtgtcagg attgattaaa atcggcgtaa tgtgctgacg
     6241 cagatactgc ttaaaataat gacgcagcca gttttgttga ttgacggaga gctggcgttc
     6301 attaatcagg aagatctggt tgcgcgccat ctccagcaat agctcgttgt agaggccgtc
     6361 gaattcctga tcggctttca gcacccggga ctgaattttg cccagtaaat ggcgggaatg
     6421 agagttggag ccttgttctt cgctaataat gatgcgtcgc ttcagttcag cgaagcggac
     6481 tttatagaac tcatcaaggt tattggaata gatccccagg aaacgcatcc tttcaatcag
     6541 cgggttagat ttgtccgccg cttcctgaag cacgcgttca ttgaacgata accaactgag
     6601 ctctttttcg atgtatagct tttcctgacc cattaccact tttactccgt tttattcacg
     6661 ggacactgcc tggatattat ggcgagcaat attggcagat gtccaactat gccagaaaag
     6721 ggggaaaaaa actgctggag cttgcgattt acaggtgaat tgaaaaacgc cagcggcaga
     6781 gctggcgctt taactacggg gcattactcg tcggcagcgt agccctgcgg cggcagacgt
     6841 tgaccatcca gccacgcggc gttttcgtgc attttcagac gaccatcggc aaaccagcta
     6901 atcaccagtg gataaatagc gtgttcctgg gtttgcacgc gggcggtgat gtcatcttcc
     6961 gtatcaccag caaataccgg gactttcgcc tgtaaaataa ccgggccacc gtccagttca
     7021 tcggtgacga aatgcaccga tgtaccgtgc tcttcatcac cattttccag cgcctgacga
     7081 tgggtgtgta atccgggata tttcggcagc agagaagggt gaatgttcag caaacgcccg
     7141 gcatagtggg agacaaacgc cgggctgaga atgcgcataa aaccagccag cacgaccaca
     7201 tcgggtgcgt acatgtcgat ttcatgaatc aactcccggt cataggcttc acgactgtca
     7261 aacgcgctgg cgatgagcgt atgcgttgca atacccgcct ggcgggcgcg ttcaaggccg
     7321 aacgcgtcgg ccttattgct gaaaactgcc cgtacggtgc ctttaatttt gttagtttta
     7381 caggcgtcaa taattgcctg taaattactt ccgttgccgg aaataagcac cacaatattc
     7441 attattcgat aaccacgcgt tgttcggaat cagaggcttt gatgataccg attttccacg
     7501 cgttttcacc gttggagttt agcagggcga gggctttgtc cacttccgga gcaggcaggg
     7561 cgataatcat cccgacgccg cagttgaagg tgcgatacat ttcatggcgc tcaacgttac
     7621 ctgccgtttg cagccagttg aacacttccg gccactgcca ggaagattca tcaatcactg
     7681 cctgagtatt atctggcaat acgcgtggaa tgttttccca gaagccgccg ccggtcaggt
     7741 gcgcaatggc atgcacatcg accttttcaa tcaactccag cactgacttc acgtaaatgc
     7801 gggtcggtgc cagcagatga tcggctaatg gcttaccatc aagttcggtg gtttgcggat
     7861 cacaaccgct gacttcaaga attttgcgca ccagcgaata gccgttcgag tgcggaccgc
     7921 tggaaccgag tgcaatcagc acatcgccgt cgctgacttt agagccgtcg atgatttctg
     7981 atttttctac cacgccaacg cagaaacccg cgacatcgta atcctcaccg tgatacatcc
     8041 ccggcatttc tgccgtttcg ccacccacca gtgaacagcc cgattgcaga catccttccg
     8101 cgataccgct gatcaccgct gaagcggtat caacatccag ttttccggtt gcgtaatagt
     8161 cgaggaaaaa cagcggctct gcaccttgca ccaccaggtc attaacgcac atggcgacca
     8221 gatcaatacc aatggtgtcg tgacgtttta agtccatcgc cagacgcagc ttggtaccta
     8281 cgccgtcagt accggaaacc agcacaggtt cacgatattt ttgcggcaat gcacacagcg
     8341 caccgaagcc gccaagaccg cccatcactt ccggacgacg cgttttcttc actacgcctt
     8401 tgattcttcc aaccagagca ttacccgcgt caatatcaac accggcatct ttgtagctaa
     8461 gagaggtttt atcggtcact gcttgggtcc ccacgcgtta cttgcggtag aaaaataaaa
     8521 ttcggcgcaa ttctaacagg gaaagcaaac gtttgcgaga ctgctttaca caaccttttt
     8581 gcacgtcttt tgcccaggca cgcggcgaaa gaagacttgt gccagggtaa aggttagttt
     8641 tcggatggaa taatcttctt tcataaccat ttgaatataa aataacttta tctcaaaccg
     8701 ttatcatttt gactaaagtc aacgaaaaga atattgccgc cttgaagaaa ggaggtataa
     8761 tccgtcgatt ttttttgtgg ctgcccctca aaggagaaag agtatgaaga tcgtggaagt
     8821 caaacaccca ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa
     8881 gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga
     8941 cctcgaaacg gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat
     9001 caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggta tgatggacgg
     9061 tgtgctggaa aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga
     9121 aacgctggag ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc
     9181 gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt atcgcgacca tcgacctgct
     9241 gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg gtcgctgcgc cagaaggtat
     9301 cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat accgcatcga ttgatcaggg
     9361 actgaacgag cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg
     9421 tacgaaataa agaataaaaa taattaaagc cgactttaag agtcggcttt tttttgagta
     9481 aagcgcctat aacacataat acagaggata atactatgac gcgccgtgct atcggggtga
     9541 gtgaaagacc gccactttta cagacaatcc cgcttagttt gcaacatttg ttcgccatgt
     9601 ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa cccggcgact gtactgttat
     9661 ttaacggtat tggaacgctg ctgtatctct tcatctgtaa agggaaaatt ccggcttatc
     9721 ttggttccag ctttgccttt atttcaccgg tattgttact gttgccgtta gggtatgaag
     9781 tcgcgctggg cggctttatt atgtgcggcg tgctgttctg cctggtttct tttatcgtga
     9841 agaaagcggg gaccggctgg ctggacgtgc tgtttccacc tgcggcaatg ggcgcaatcg
     9901 ttgccgtcat cggtctggag ctggcgggcg tagctgccgg tatggcgggt ttactcccgg
     9961 ctgaagggca aacgccagac tccaaaacca tcatcatctc tattaccacc ctggcggtca
    10021 cggttttagg ttccgtgctg tttcgtggtt tcctggcaat tatcccgatt ttaattggcg
    10081 tgctggtggg ctacgcgctc tctttcgcaa tgggaattgt cgataccacg ccgattatta
    10141 atgctcactg gtttgcgctg ccaaccctct atacgccgcg cttcgagtgg tttgccattc
    10201 tgactattct gccagcggcg ttagtggtta ttgccgaaca cgtagggcac ctggtagtaa
    10261 cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg tctgcaccgt tcgatgtttg
    10321 ctaacggcct gtcgaccgtg atctccggct tctttggctc tacgccaaat accacttacg
    10381 gagaaaacat tggcgtgatg gcgatcaccc gtgtttacag tacctgggtt atcggtgggg
    10441 cggcgatttt cgctatcctg ctttcctgcg tcggtaaact ggctgccgct atccagatga
    10501 tcccattgcc ggtgatgggc ggcgtttcgc tgctgcttta tggtgtcatc ggtgcttccg
    10561 gtattcgtgt tttgatcgaa tcgaaagtgg attacaacaa agcacagaac ctgatcctga
    10621 cttccgtgat tttgatcatc ggcgtcagtg gcgcgaaggt aaacatcggc gcggcggagt
    10681 taaaaggtat ggcgctggcg accatcgtcg gtatcggctt aagtttgatt ttcaaactga
    10741 tcagtgtgct gcgccctgaa gaagtggtgc tggacgcaga agacgcggat ataacagaca
    10801 aataatgtac cgaccgggca gtgttgctgc ccggttcaaa catcatggga ttctgtggta
    10861 aacttctcgc gattttgtga aatcctggtt gaggtatctc tgaacacacc ggcacagctc
    10921 tctttgccac tttatcttcc tgacgacgaa acctttgcaa gtttctggcc gggggataac
    10981 tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg aacatagcgg ttacatctat
    11041 ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc acgcggcttg cgcggaattg
    11101 tcgcagcgtg gcgatgcggt gggctatgtc ccgctggata aacgcacctg gtttgttccg
    11161 gaagtgctcg acggtatgga gcatttgtcg ctggtctgta tcgacaacat tgagtgtatt
    11221 gcaggcgatg agttgtggga gatggcgatt ttcgatctct acaatcgaat tctggaatcg
    11281 ggcaaaacac ggttgttgat caccggcgat cgtccaccgc ggcagttgaa tctgggatta
    11341 ccggatctcg cgtcgcgact cgactggggg cagatctaca aattgcagcc actttctgat
    11401 gaagataagt tgcaggcgct acagttacgc gcgcgtttgc gtggttttga actgccggaa
    11461 gatgtggggc gtttcttgct gaagcggctc gacagagaaa tgcgcacgct atttatgacg
    11521 ttggatcagt tggatcgtgc gtcgattacc gcgcaacgta agctgaccat tccgtttgtg
    11581 aaagaaattc tgaagttgta gataaggtgt ttattgtcgg atgcggcgcg aacgccttat
    11641 ccgaactacg cgaagttaat ccatagtatt cgtaggccgg ataagacgtg gcaagcgtcg
    11701 catccggcaa tgtgcttcaa ctagcgatgg agcacatctt aacccacgat ttccagtacc
    11761 tgctccggtg gacgaccaat ccgcgctttg ccattcgcga ccacaatcgg gcgctccatt
    11821 agcttcggat tatccaccat tgcctgaatc agcgcctctt cgctaagcga actgtcagcc
    11881 aggttcagtt ctttataaag atcctctttc tggcgcatca attcacgggc gctattcatc
    11941 cccagcattt tcagcaaatc gcgcagcgtt gccgcatcgg cgggtgtctc aaggtagagt
    12001 accacttctg gctccacgcc gttttctttc agcaggttca gcgtttcccg gctctttgaa
    12061 cagcgtgggt tatggtagat ttttacctgt ttggtcattg ttcttccttt aatgcgaatt
    12121 acatcttggt ataaggctta aagcgttcct gcagctggcg caactggtcg atgcgcgcat
    12181 cgtaacgcgc ttgttgcagg ctgcctaatt tcacctgcga actggcgcta ctcaacagcg
    12241 aaatggcctg atcgagtcgt ccggcgagcg cataaccttc tgctcgcgca gccagctcct
    12301 gatcgcggtt atttagcgcg gcttccgcct gtgccagtaa atcccagccg ttgctgtcat
    12361 ctttattatt aaaggtgtag cgattcagaa tattggccgc ttcttgtggt tgaccgcctt
    12421 gcagataagc gttcgccagg ttgagctgca acaccggatt ggtgcgtaaa tcgcgggcat
    12481 ttttcagacg attgatcgcc tcattggctt tgttttgccc aagatcgata tcagtagcca
    12541 gatcgagata ccatgcgttg ccaggttctg ccgccagtaa cggttgcagc gttttacgcg
    12601 cctcgtcgta tttattggct tccatcgcct gtaaagcacg accatattgt gctgctcgtt
    12661 gctgacgaac gtttcctttc gcccattcat ccagcaaatc actggtgagc tggttacgtc
    12721 cggaattata catccccagt gtgcgcgctt tcgccagata gaaatcttcc gacgactgca
    12781 ccaccatcgg gcgcatctga ttagcacggt tgcgggcatc tgccagacga ctttccggca
    12841 acgggtgagt caataaaatt tccggcggac gcgaggagta acgcgcctga tcgagtaatt
    12901 tttcgaggaa ggttggcatc gcctgcggat cgaatcccga gcgttgcagc acctgaatac
    12961 caatgcggtc cgcttcctgt tcattttgct gggtgaaact gatcatcccc tgacgcgttc
    13021 ccgccagtgt accggtcagc gccgccatcc ccgcctgcgg actggccatc gccagtaaaa
    13081 tagaacctaa cgcgccgacc caggtcagcg gcgcgctgcg ctgctgatct tccatcgctc
    13141 gcgccaggtg acgttgggtg acgtgggaga tttcgtgcgc cataactgaa gccagttgac
    13201 tttcgttatc ggaataacgg aacagggcag agtgcagcac cacgttgccg ccaaagaaag
    13261 caaaggcgtt aatttcgtcg ttgttgatca gaaagaaatg aaacggtgtc ttaaccgaat
    13321 tggcatgcga aaccagacgc atccccagcg aattaatata ttgcgttaac agcgggtcat
    13381 taattaacgg cgcgctgccg cgtagctggc ggacataata gtcgcccatc tgcatttcct
    13441 gaccaatgga aagcgtgctt cctgcggagg ttcccatatc cggcaaggtg tctgcgctgt
    13501 cggcaaacgc cggggctacc tgaccaatgg tcatagcagc aatgagggtt gcaaccaggt
    13561 tttttttcaa ctgcctgaac ataacctcta tcctgtattt ctgaagatag tcatttgacc
    13621 gatactgtta aatatcgttc ccaccgcgcc gcctttgagt gtaacggcga agattagccc
    13681 actcaaatga cttttcagtt tcctgaatcc gtcataaaaa gcgaagtctt ttttgtgaca
    13741 tttcgataca attgcggatc gcaatcccgc tcttgaggtt cagggaaggt ctttatgctc
    13801 gaaatgttga tgcaatggta tcgccgccgt tttagcgacc cggaagcgat tgccttgctg
    13861 gttattttag ttgccggatt tggcattatc tttttcttta gtggcctgct tgctccgttg
    13921 ctggtggcta ttgtgctggc ctatttgctg gaatggccaa ctgtgcgcct gcaatctatt
    13981 ggctgctccc gccgctgggc gacgtcgatt gtattggtgg tttttgtcgg tatattgcta
    14041 ctgatggcgt tcgtggtact gcctatcgcc tggcaacagg gcatctactt aattcgcgat
    14101 atgccgggga tgctcaataa gctttctgac tttgccgcca cgttgccgcg ccgctatccg
    14161 gcgttaatgg acgcgggcat tattgatgca atggccgaaa atatgcgcag tcggatgctg
    14221 accatgggcg attcggtggt gaaaatttcc ctcgcctcgc tggtcggttt gctgaccatt
    14281 gccgtctatc tggtgctggt gccattgatg gtcttcttcc tgctgaaaga caaagagcag
    14341 atgctgaacg ccgttcgtcg ggtgctgccg cgcaaccgtg gactggcagg acaggtgtgg
    14401 aaggagatga atcaacaaat caccaactat atccgcggca aagtgctgga gatgatcgtg
    14461 gtggggatcg ccacctggct ggggttcttg ctctttgggc tgaactattc gctgctgctg
    14521 gcggtgctgg tcggcttctc ggttcttatt ccgtacattg gcgcatttgt ggtgaccatt
    14581 ccggtggttg gcgtggcgct attccagttt ggcgcaggca cggaattctg gagctgcttc
    14641 gcggtgtatc tgattattca ggcgctggac ggcaacctgc tagtaccggt gttgttctcc
    14701 gaagcggtta acctgcatcc gctggtgatt attttatcgg tggtgatctt cggtggtttg
    14761 tggggattct ggggcgtatt cttcgccatt ccattggcga cgctgatcaa agccgtgatc
    14821 cacgcctggc ccgatgggca aatcgcgcaa gaataatctt cagcccgacg aactgtttcg
    14881 ccgggctgat ttttcaggat tcctgacgta aataaatagc ccgataacac atactcacca
    14941 gcactgcacc gccgataata ttacccagca tcaccgggag cagattggcg gtaataaaat
    15001 ggctgaccgt cagtgccgga aaattgtctg cgctactgtg cgccagttgc cagaaggagg
    15061 taggggcgaa atggcgaatg gcaatggcga acggaatcac aaacaaattg gcgatacagt
    15121 gctcaaagcc actggcgaca aacagggtga tgggcaaaat catggcgacg attttgtcgc
    15181 ataacgaacg cccgcaataa ctcatccaca gcgccaggca aaccatcaga ttgcacataa
    15241 tgccgaggct gacagattca gtaaatgtat gatgcatttt gccctcggcg cagtgtaaaa
    15301 ccgcgactcc ccacatcgcg ttttcactca tcaccagccc ggaaaaccag attaacaaac
    15361 tgaaacaggc aatacctgcc agattaccgc aggccaccag aagtgcgtta atcagccaag
    15421 ttcgccaact aataacgccc cgactttttg ccattaccgt cattaccgac gaggtgaaca
    15481 ggttggtgcc gcaaaccgcc agcaaaataa agccaagtgt aaagcaaagg ccgcccacca
    15541 gatgggttaa tgcctgtgaa gacggggtat cggcgataac ggaaaggtaa aaagtaaatc
    15601 cgatcgccat aaacacgcca gcggacatcg ccattaaaaa gaccgacact ttactgcggg
    15661 caattttatg ggcggcaatc cgttcagcga ttgccgcttt tctggcgctc aactgcaagt
    15721 cgaagagagt ttgtttctca tgcatactcc tgcaaaagca gattacaaca cctcgcgaac
    15781 cgagattccc agacgctgca ttcgtgacag cagcgtggtg cgcttcatcc ctaatcgtgt
    15841 cgccgcgcca cggggcccgg caacaatgcc attggtttct cgcaatacct gaacaatgcg
    15901 ctgacgttct tcttcgtcat tctctggcgt cgtcgggtgc agcaactgcg ccattgaact
    15961 gcgaagcgct gaatcttcat ttagcgtcgg cagtaaacgg ctttgtcgga cattaagatg
    16021 taaattcaga ctgttaccgc gagtcagcag taccgctcgc tcaatcacgt tttccagctc
    16081 gcgcacgttg cccggccagt cccacgacat caactggcgt agcgcctcgg tcgggatggc
    16141 gtcaattgaa cgattcatat ggcgcgccat tttttgcgtg aaatgctttg ctaaaagagg
    16201 gatatcttcc ggacggtcgc gcagcggcgg caattccagt gggaagacat tcaggcgata
    16261 aaagagatcg ctgcgaaact ggcgatcttc aaccatttgc cacaaatcac ggttggtggc
    16321 ggcaatgact ctgacattta ccgggatcgt tctactcccg ccgagacgct caatctcccg
    16381 ttcctgcaat acgcgcagca gtttaggctg aagttctaac ggcagatcgc caatttcatc
    16441 gagaaacaac gtgccgccat cggcaatttc aaaacgacca cgatgggtat taatcgcacc
    16501 agtaaacgcc cctttgtcat gaccgaataa ctcactttcc agaagactgg cggggatggc
    16561 agcgcagttg attttgacca gcggcttgtc gcgtcgcggg ctaagttgat ggatcgctct
    16621 ggcgatcacc tctttgccgg ttccggtttc accgcaaatc aacaccgtac tgtcgctctt
    16681 cgccacaata tctacctgct ggagtaggtc ttccattgcc tggctttgat agataatgtc
    16741 accgatgccc agattcgaaa ggagctgctc gctaagctgg tggttttctt gctgcaaact
    16801 ttcctgcaaa tcggtcatgc tacgccaggc atcggcattg ccaacggcaa tagcgatgcg
    16861 atcggctatg tgttgtagta gctggcagtt ttcctcacta aagagagtgg aagaggtatg
    16921 cgccagcaac aatgcacccg gtgtatggtt gccaaaggta agcggtataa ggagcgcaga
    16981 ttcgcagccg ttagatgcaa gtaagagtaa taacgggtcg cgttgccaga gaaacagatc
    17041 gtctgcacgg tgcgtcaagg tcggttgtcg ggtttgtagc gtttgtgtca gcaatacact
    17101 gtcgccaggc atactgcgtg gcagacacgc acaatgtgag gcagaaagat cgctgcacca
    17161 caggctaaac ttctcgttct ttcgatgatc gcccagtacc atactgactg aagccagacc
    17221 gaaaaaatga tggatctcac gagcgacgtc agcgatcaga tcgtcgagat caaaatgtga
    17281 cagcactgca ttagtaatat ccaccagtac ccgatgatga tcgcgatctt tctccagcaa
    17341 acgcagcgcc acctgctgtt cttcatcatt gagccatgat tgcagaaccg gggtgattat
    17401 ccgcgccagc cagttaaaca gtgtgcaatc ctggggagta aaacggctgc cgtgggtacg
    17461 aataaagcgt agctccgcca ttccactact gccgagaagg acggcgtgac gcgaataatc
    17521 gaccatctcg ccctgatact ctgttgttgc cgtcacatcg ctgccatcat catggtagcg
    17581 gtaatagaag ttgcttttga gaacatgcag ttcaataagt tgtaccgggc tgaacggcgg
    17641 cggcatcggg atgaaggcgc gtaataaaga cgccttaccg tgtttctgtg ctaaccgctc
    17701 cgcgagcatt ccgtttaccg cttcaattgt tattccttgt ggcggggcaa acatcgcctc
    17761 gtctgacata gccatcaaca gtccttatta atgagaaatc cttctcacgc aatttttctg
    17821 gccatcagat agatggccgg ttcctgcaca atttcatcca acagatgaat gaattgaatg
    17881 ctacgcgcct gccacacaga cgtcgcgtca tctgccagtg gcgcgaatgc ccgggtcaac
    17941 agtgcggtgt ggctggcgtc aataacaatc tcaccaaaag tcatcacccc cagcatctta
    18001 cgtcgggctt gctcctcttc gaccagtgca agccaatctt cgtattccgt cagtgggcag
    18061 cggaaggcga cattcagaca atcaatcacg ccaacgtgat gaccgatagc cagcgagtaa
    18121 tacattacct gggagctgtg ttccggcatc tcgtcgctac tggcgacaaa cttttttcgc
    18181 agcgtccaga aaacaatttc cccgcactcc tcagccatgt aacccccgga gaacgcgaaa
    18241 gagttgctga acgatactat tgagacgcgg atcgtcagct tcgcgcaacc aggtgttaac
    18301 ccgatttcct gtaggatcgt cgcttaaatg gcgcaggagc cgatcgcaaa tttctcgccc
    18361 ctgacgatag cccgccagct gccgcgcttc tcgctcaatg gcgatacgct gtgatggcgg
    18421 gatctgcggc cacagcggtt gcatggtcac cccggtggga tcgcgataat ccacagcgtg
    18481 aatcttctgt tgcagcaaac cgagcgccac ggcgaaaccg tgaatggtgg cggccggtgt
    18541 tggcgggcag ccggggatcc aaacatcaat ggggacaatg gtgtcgctgc cgccccagac
    18601 gctgtagaga tcgtggaaaa tccgccgccg gcaccgcacg cgccgtagga aacacagatt
    18661 ttatgatcgg gggcagactc atacgcccga agtgcaggca tacgcatcgc ccgggtgagt
    18721 gcgccagtaa ataacaaaat atcggcgtga cgcggtgatg aaacaacctt aatgccaaaa
    18781 cgttctgcgt cgaatactgg tgtaatggcg gcaaaaattt cgatttcaca ggcgttgcag
    18841 ccaccgcaat cgacgcgata aacgtaggct gagcgacgga tatcctgtag caaatgccgc
    18901 ttcatctttt gcgtttgctc gtccagcgtg atgggctggc tgacatattg cgtaagcact
    18961 ggactcatag ctgctcctta gccaccagcg gtacatctgt atcgtcgttg agcagcgtcg
    19021 cgcgttgttt gcactccggg cagacgctcg cttgcgccca taacatttcg cggttttgtg
    19081 gcgcattttg ttgctgtgcc aacaattcag cagccagtgc gacggttttt tgctgggcaa
    19141 acgggcgttc gcaacggctg caacgttgta gatggaacgt cgcgcgggta tagagatcgg
    19201 ctttattggt gacggtcagt tcaaagttat tggtaagctg atggctctgg tcgggcacac
    19261 ttcttcacaa cgtccgcagt aaatacaacg cctcagatag agctgccagg tgcgcgaatt
    19321 ttgctggtcg tcggtctgga tagtcagcgc atttgccgga caagcacagg cgccgcaggc
    19381 aatacattgg ctgggcatca ggtccggttt cccgcgaaag cctgggctga cctccagtgg
    19441 cgcgaaggga tatttcaccg tcgcggttcc ggcgcgcata atagttttca gtaacttcag
    19501 catcttctgt ccttacttca gcggcgaacg gttacgatca atgccgtagc gttcgatctc
    19561 tttatacggc acggtttttg actggcgctt gcgcacatct accagcgtca cacggtcggt
    19621 acaggagtag cagggatcaa ggctaccgat aatcagcggt gcgtcagaaa cggtattgcc
    19681 gcgcagcatg taacgcaaca ccggccagtt ggcgtaggtg gcggcacggc agcgccagcg
    19741 gaacaatttt tggttatcac cgagcatgct ccagtgcacg tcttcaccgc gtggcgcttc
    19801 aacaaagccc agcgcgaatg cgtgaggttt atagctaaag ccttcggtca gcagtggggt
    19861 atccggcatg ttgtcgagag caaattctag cattgccagc gaatcaaacg tctctttgac
    19921 acggaccatc acgcgggaga aaacatcgcc gccggtaaag gtaaacagtg tttttggaat
    19981 attgccgtag tcggcgtagg ggtgatcaaa gcgcaaatca cgggcgattt gtcggtcgag
    20041 aatgccaatg ccctgagtgc gttgttccat attcggcgta gcaagcagca tctctaccag
    20101 ctccgacacg tcggcgcgca tctcgcgcac cagtttcagc gtttgcagac gttgctcttt
    20161 gagaatatcg cggcgaacac caccaatcag attcagaccg taggttttac gcgacccgat
    20221 cagcaattcc gccatcgtca tcgacttttc ccgcacgcgg aaaaattgca taaagccggt
    20281 atcaaaacca acgaaatggc aggagaggcc aaggttaagc aaatgactgt gtagccgttc
    20341 gacttccagc agaatcgagc gaatagtatg tgctcgttgc ggcacctcaa tccccagtgc
    20401 attttcaacc gagttggtat aggccacact gtgggcaaaa ccgcaaatcc cacacacgcg
    20461 gtccgataag aaggtcactt cgttgtagcc catccgcgtt tctgccagtt tctccatgcc
    20521 gcgatggaca taaaacaggc ggtaatcagc atcgacaatt tgctcgccat ccacaaacaa
    20581 gcggaagtga cccagttcat cggaggtgat atgcagcggg ccgacaggga tcacctgcgc
    20641 atcgctgttg ccctcattga taaacggata cgtttcggaa tcagtcgtcg gttcagggcg
    20701 cagtcgataa tccatcgcat ctttgcgcag cggatgcata tcttccggcc agtcatcggg
    20761 caacaccagg cgacgctgat ccggcaggcc aaccggaatc agcccgtaca tatcgcgaat
    20821 ttctcgctcg ccccagaccg cggcagggac gcgcggtgtg actgacggaa actcccgact
    20881 gtcggcatcg accagtgcct tcaccacgat ccagcatttt tcggcccctt ccattgaaag
    20941 ggcataataa accgcgtaat gaccgttaag tgtccgctcg tcgttgccaa acaggactgg
    21001 aagccagcca tcatgttgat aataaagata atgtacaacg tcaggcagca gattgatttt
    21061 caccgtaatg gtgacctgtt caggcgtttg tcgctcttca tccagcaccg cgccggggaa
    21121 ctgcgttttc agggcggcga gaatcgcttc gccacgattt gacgatgaat taacgttcac
    21181 tgtgtttctc cgaaacagat gcggtgcctg aaggcaaaaa atcatgccag gtgctacgtt
    21241 gtgcaggcag gtcgtgcgtc cctgagagga ctatagtgga agcgcccgcc aggatcctga
    21301 tgacaggttg tggaatatgc gttcccatcg ccagcatcat gaccagcaga atcaccattg
    21361 gcgaggtggt caaccagccg agatcacccc ggttaacggc ctgcggcggt ttcgccatta
    21421 acacccgcgc tgccatccgt accaggcccg ccagcaccag cgttaacagc aataacagca
    21481 ggacgataat cagcaggtga ttacgtgcca gtccggcggt aatggtcata aattcgctaa
    21541 gaaaaatgtt gaagggcggc atccctgcca gcgccagcgc cagcgcaccg ccgccaaaca
    21601 gcacggcggt aaatggcatg attttgagca tcccacagac gacgttgaga tcgcgcgtgc
    21661 cgtacttgag cagtacattg ccggaaccgc agaacagcag cgtttttgcc agactgtggt
    21721 ttaaggtgtg cagcagcgcg gcaaaaattc ccagtggccc gccaatgcct agcgccaccg
    21781 cgaccagtcc catgttctcc acgctggagt acgccagcag acgcttaatg tcccgctgta
    21841 ccagaatgaa aaatgccgcc acggcaaccg acaacatgcc gaagatgagc aacaaccggt
    21901 tggggaaatc gctgccgatg gcctggcaga taatgatgta atagcgaatt agcaccaaca
    21961 gcgcgcagtt cagcaatacg gcggagagca gggcgctgac cggactcggt gcttcactgt
    22021 gagcatccgg cagccaggcg tgcatgggaa atagcccggt tttggtgcca aagccaatta
    22081 gcacaaacac aaaggccaac agcattaatg ttgggtcaag caaggacgat tgcttaagaa
    22141 cctcgctcca gaatatcgcc atttctgcct gcggcataac gctggcgacg ttggcgtata
    22201 ccagcacggt accgaacaga ccaaaagcga caccaacagt acaaataatg atgtacttcc
    22261 atgctgcttc cagcgatgaa cgctgaccgt aaatgcctac cagaaacgcc gagcttaagg
    22321 tggtggcttc gatcgccgcc cacatcacaa tcaggttatt gctggtaaca accagcagca
    22381 tggtaaacaa aaacagatgg aagaaaccgt agtaatcgca cagcgtaacg ggggaaagct
    22441 cgccgtgtgc cacttcatga cgcatgtagc caatcgagta aacaccggtg agaaagccaa
    22501 tcacaccaag aatggcgagg aacaaaccgc ccagaccatc aatatgaagc cacagtcccg
    22561 cagcgaatat ttctcctgca tcagcggcag tttggaccac ccagagtgcc agaatcagca
    22621 gcagtgtgat ccctaagcta tgtaatacag tcaccgtaca agtcgcagag agcccccgtt
    22681 tccggcaggc aaaacagagc agcgaaaaaa gcagcggcgt gagcaggagt aaagcgaaca
    22741 tcacagaata actcatctca ttatcccttc agcgcggtca agttgttcac gtccagcgtg
    22801 ccgtgggtac gccatatttt tcttgccagt aacaccatca caatgacggc gaagatggcg
    22861 tcggtagcga tacctatttc caccagttcc ggtgctcgcc aggcaagaag cgccagcacc
    22921 agatgggagc cgttttccat caggcagtaa ccaaaaattt gccgcaggat attgcgctgg
    22981 ctgacaatgc acagcaggcc aagcagaaaa tgacctaacg ctaccgccag cgccggtttc
    23041 agcccggtag ccatcggtag cttcacgggc tgaacgacaa atgcgcaaag caggacaatt
    23101 aacgccgcga gcagtgccat cattgccgga ccgaataacg ctttttccgg gatgttctgg
    23161 ggaatatttc gtgcagcgta agtcatgatt aacggtacca gcagcacttt ggtgataaag
    23221 gcgctggcgg accagatcag cagttgctct gcggcgaaca ggcacgagag agtggcgaaa
    23281 atagacacca gcaccagcga ctggcaggcg taaaatccgc atgacaggcg atagcttttg
    23341 acgctaatca caaacagcga tgtcagcatc atcagtcccg ccagattatt tacgatcata
    23401 gaaccggtca ttcatcatct ccttactgca accaggtcgc ggcgattacg ctggaaatca
    23461 gcaacatcac aatcagtaca atcaacacca ggcgcattga ccctggcagc ggtgcggcat
    23521 cggcgacggc ctcgctcggt ttgccaggca caacgcgacc aaaccagcga ataaaccagg
    23581 cgaaactggc gaccgattca atcatcagca gaatcatggc gggcagcagg atccagtact
    23641 ccactgacaa cgcaaaaccg gcagcaaaca gcgggaattt actgaagaag ccgttgaacg
    23701 gcggcacgcc ggtaatcgcc agcgctgcca cgcagaaacc cacgcctggc aacggcaggg
    23761 tgtgcaatac gccacgcaga cgcggcaaca agcgcgtgcc gcagctgtaa ctcagcgcac
    23821 ctgctacaag gaaaaacagg cttttagcga acgcgtggtt gacgatgtag gcgatgctgc
    23881 cctccagtgc cagccgccca ctttcaccat cgatgcggcg tggagatagg cgctgatcgg
    23941 tgttggcgct tccattgcat ccggtagcca cgcttgcatc ggtagctggg ccgatttccc
    24001 ccacgcggca aacaggatgc cgccataaac cagataacgt gcgtcgccat gtaactcgct
    24061 catcgcgcta agcacaaacg ttccggtctg caaaaacagc gtggcggcgg caagatacaa
    24121 ccccaacgaa ccgatatggg tgataagcaa cgcttttagt gctgaacgct gcgctttatc
    24181 gctctggtaa taactgatca acgcccagga gcagccgccc gtaatttcaa aaaacaacaa
    24241 ctgaccgagc agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaccagcaa
    24301 aaatgcgtaa taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata
    24361 acccgtggag tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt
    24421 actcacgcga tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaacgtcaa
    24481 cgtgacggca actttatcgg cctgataaaa cgcggagatc agcgacaaca tgcacagcgt
    24541 ggtcagtgcg gcgaacaaaa cccccattcg gcggcccgac gttgtggcga aaacgaaacg
    24601 accagtgcgc cgataaaagg cagcattaac gtcgtcagag caagattttc catattccgt
    24661 cagtgctcct tacagaccgg ttaaccagaa gacccatgca agcgcagcaa ggctgaagcc
    24721 aagccaggtc acatggtgaa tgagtaaaaa acgcccgcgt gccagcgtgt tttctgcgat
    24781 tgaggccagt acaaaaatca gcaaaacctt gagcagcgta acgacaagtg aagtcagcag
    24841 gcaggcgaga gaaagttctt gcgcgcgccc aaagggcaga aacagggcca caaacagtgc
    24901 tgccatcacg acctgtttca gccccagccc ccatttcgct agcgccagcc cggcaccgga
    24961 atattcggtc agcgggcctt cctgtaattc ctgttctgct tcagcaacat caaagggaat
    25021 ttttcccatc tcaatgaagc aggcaaaacc acaggccagt aacgccagta cggtggttag
    25081 cggcgagttc cagcccgttg ccagcgtatt gctgatcatc tcgatatgcg tggaacctgc
    25141 tatcagcgcc aataccagca gtgagagaat gagcatgggt tcgaccagaa tgccgagcgt
    25201 caactcgcga ctggcaccga ctcccgcaaa cgggcttccg gtatccagcc cggaaagagc
    25261 aaagaaaaaa cgaaacaggg caagaagata gataagggtg atcagatcgc cgccgcccgc
    25321 aaaaggggaa acggtaataa aaagtggtag ggccatcgcc agcaccagca tgctgctgat
    25381 taatacccac ggcatcaggc ggaacatcag acctgaagat ataggcgcaa cttcctggcg
    25441 tttaaacagt ttgtggatat cgcgataatc ctgccagatc cccggcccgc ggcgggagtg
    25501 catacgcgcg cgtatctgcc gggaaatacc cgtaaaaagc ggggttagca tcagcagaat
    25561 cacggcctgt gctaacgcaa aaatgaccag atatccgtcg taaagagttt gtctcatggt
    25621 gatttcctta gacggcaata gcgattagca gcacaaccag tgcggcgacc acatacaggc
    25681 aatagagacg aaagtcgccg ctttgcagat gctgtatttc tttggccagc cgttgggtgg
    25741 cgctcacgat ggggcggatc acccgctcat cccagaaggg ttctgtgctc tgagccctgg
    25801 cggtgacgtg cgcaagacct ttatttagcc tcagcgtagg gtcgaactgt tgtcgtagac
    25861 gaaatagcgc agaaaagacc acacgcagcg actgcatcac gccattgcct gacggggcca
    25921 tcgcattttc ccagccgtag ccgcatgccc aggcatctcc tgtgcgacga aacgctgcgc
    25981 ggcgcgaacg acaccacagc cagtaaaggc caggcagcaa aggcatcgcc agtagtaaca
    26041 gcaacagtaa tgagggggtg acctgtgtat gaaacgtgcc gggtacaagt gctattccgc
    26101 tggcgacagt gacgggaggg gtattggtaa acgcatgggc aatatgcatt atcttcggtg
    26161 ccagccaact ggcactaagc gcaattaata cgcagagtgc cgcgagcagt agcatggcga
    26221 cgatcatcgt atttggcact tcctgtgcct cttcagcgtg tgtactgcgc ggcgcaccac
    26281 agaaagtaat accgtacatt tttacgaagc acattactgc cagcccacca gtgactgcca
    26341 gcattaccat agcaatagga cccgcaagtt gtagcgctac ggcttccaca cgacttagtg
    26401 agaacagcga ctgccaggtg taccattcgc tgataaaacc attcagcgga ggaatggctg
    26461 atatcgcgag gcaaccaatc aggcatgctg cggctgtcca cggcatccgt ttcgccagtg
    26521 cccccatttt ttccatgtcg tgggtatgca aacgcgaaat aatcgcgccc gcgccgagaa
    26581 atagcagccc tttgaacagc gcatggttga gcagatgaaa cagtgcgccg agcagtccaa
    26641 ccacggtgag cagcgggtcg tgcagtgaca gaccgaccat cgccacaccg actgccagca
    26701 aaataatgcc gacgttttcg acggtactcc aggccagcag ccgtttgata tcctgttccg
    26761 ccagcgcata cagcacgccc aggagcgcgg ggattgcgcc gatcgccatc accagaatgc
    26821 cccaccacag cggcaaaccc gtttgcgcca gcaggtccat cgctactttc aggatgccga
    26881 aaataccgat tttgaccatt acgccagaca tcaacgccga agcgtgcgat ggtgcggcag
    26941 ggtgagcgcg cggcaaccag ctgtgcaacg gcatcatccc ggctttcgcg ccaaaaccga
    27001 aaaaggccag caggaacacc gccgacgcca gccccggaga aagtgaaagc gtgcggaaac
    27061 tggcaaaatc gaggctgccg ctttcgcgcc ccatcagcaa gaaggcgatc ataatcagca
    27121 ccgatccggc gtgggcgata aaaaagtaga gcatgccagc gttgatcgac gttttatcct
    27181 gcctggcaat gaccagaaac caggaagaca gcgacatcat ttcaaacagc acgatgaacc
    27241 aaaaagcgtt gtccatcacc agcagggcaa ccatcgatgc gatgaaaaga ttcataaaga
    27301 agcccatcgc cgccgcgcct ttgccctcgt attcgcgcat ataagtcaat gaatagagcg
    27361 aacaaaccac caccagcaag gagataacca gcaccataaa tgccgagagg ctatccattc
    27421 gcagggtaaa atcggcaaag tcaaaagggg tggcaaaacg ggcgactaaa gttacaccag
    27481 catgtaattg cgtaattccg ctgatgatgc caatcacgcc gcccaccagc gatgtgatgc
    27541 cggaaagctt aatagccagt ctgtccagac cgagtaaaaa cagcgaagcc agactggcaa
    27601 acagatagag gatcagcgac caggttaata attgcagggc atccattagc gttgctcctg
    27661 agtgagggaa aggggatcct ccccgccatt ggcaaaccag cttgcggcaa ggcgctgttt
    27721 ttctttcatc tgtcgttgca ggctatcgtc ggtgatcagc cgtaacgcct gattcgggca
    27781 agcgcgaacg caggccggac cttctggcaa gaaatcacac aggtcgcatt tcaccgcgac
    27841 ggtctggaca cccggttccc agcgcagcaa tgcatgttgg ggcggcacgt tttcttcgcc
    27901 gtctttgagt gagccttcag cctgaaaaac atattgcgca ggggcattca ccggacggct
    27961 tcctgaagcg ctgattgcgc caaatgggca gaccacggcg caaagcttgc agccaataca
    28021 gaggctttcg ttgagttgga tcgcatcatc cctctgggag atggcattaa ccgggcagac
    28081 ctgcaggcaa ggggcttcct cacagtgatg acacacgaca ggggcagtga gtgttgacgt
    28141 cttcgccagg gccaggcgcg ggtgttgctg taaaccttgc gttttatgca cgtccgaaca
    28201 ggcagcgaga caggtattac atcctgtaca ccacagtggt tcggccacca caaagcggtt
    28261 catgtcggct ccttgaaaat gatatgaaac attgcatttc atattgcata aaggatgcca
    28321 ttctgattaa ctcaatatat tcaataagtt atgtattgac tggattctga acggtacaaa
    28381 aatgatggtc attaacgttc agcaaaaaat ggattcgaca cccgtgtcgc aggggaaagc
    28441 gagaatgtga tcttgatcac gagaagtgag gtatgctggc cgcaagcgca gccagcacgg
    28501 aatggagcaa agtaatcagg cgtgttcttt cagccagttc agcacaacgt cgtggtgatt
    28561 gctggttttg aaatcgtcaa agacatgttc gattttgcca tcagcgtcaa tcaggaagct
    28621 gatgcgatga atgccatcgt aggttttgcc catgaaggac ttttcacccc agacgccgaa
    28681 ttgttcgcac acctggtggt cctcatcaga taggagcgta aagttaagca gctctttttc
    28741 cgcaaaacgg gagagttttt cgggtttatc ggtgctgata cccagcacat caacgcccgc
    28801 ttttttcaac tcatccatgt tatcgcgtaa gccgcaggcc tgtacggtac agccgggggt
    28861 catggctttc gggtagaaat aaaccagaac acgctgtccc tggaagtcgg tcaaattaac
    28921 ttgttctccg tcttgatccg gcaagctaaa tttcggtgcg atatcaccgg ctttcagtgg
    28981 attcattact taactccatc ctgttcatca tgttgggaat aattgacgac gttaatactg
    29041 ccttgtgcat tgagttctgt acatagggct ttgaacgctt gctcaatatt tgctgcgtcc
    29101 gcagatgcgg ggctgtgggc ggttatctga atatgcaact gcgcagcccg ttcattttca
    29161 gcaggttgcg tgcgcgacac cagctccgca atgttcatat gatgcgcgtc gaaaagtgct
    29221 gtgaagcgtt caattaaatg cggggagtct gccacatcga cctgaaccca gacagatgct
    29281 ggcattggcg gacgcggacg cgccgtcgtg cgcttcatca cgattaaaag atccagttcg
    29341 gcacctttca acggtaacgt tgattcaatc agagtaatgg cattccatga accggaaagc
    29401 agcataataa acgtgaactc ttctcccagc atcgccaggc gactgtcttc aatattacag
    29461 ccgcaactac tgacatgacg ggtgatggtg ttcacaattc cagggcgatc ggcacccaac
    29521 gcagtgatca ccagataatg ttgcgatgac agtgtcaaac tggttattcc tttaaggggt
    29581 gagttgttct taaggaaagc ataaaaaaaa catgcataca acaatcagaa cggttctgtc
    29641 tgcttgcttt taatgccata ccaaacgtac cattgagaca cttgtttgca cagaggatgg
    29701 cccatgttca cgggaagtat tgtcgcgatt gttactccga tggatgaaaa aggtaatgtc
    29761 tgtcgggcta gcttgaaaaa actgattgat tatcatgtcg ccagcggtac ttcggcgatc
    29821 gtttctgttg gcaccactgg cgagtccgct accttaaatc atgacgaaca tgctgatgtg
    29881 gtgatgatga cgctggagct ggctgatggg cgcattccgg taattgccgg gaccggcgct
    29941 aacgctactg cggaagccat tagcctgacg cagcgcttca atgacagtgg tatcgtcggc
    30001 tgcctgacgg taacccctta ttacaatcgt ccgtcgcaag aaggtttgta tcagcatttc
    30061 aaagccatcg ctgagcatac tgacctgccg caaattctgt ataatgtgcc gtcccgtact
    30121 ggctgcgatc tgctcccgga aacggtgggc cgtctggcga aagtaaaaaa tattatcgga
    30181 atcaaagagg caacagggaa cttaacgcgt gtaaaccaga tcaaagagct ggtttcagat
    30241 gattttgttc tgctgagcgg cgatgatgcg agcgcgctgg acttcatgca attgggcggt
    30301 catggggtta tttccgttac ggctaacgtc gcagcgcgtg atatggccca gatgtgcaaa
    30361 ctggcagcag aagggcattt tgccgaggca cgcgttatta atcagcgtct gatgccatta
    30421 cacaacaaac tatttgtcga acccaatcca atcccggtga aatgggcatg taaggaactg
    30481 ggtcttgtgg cgaccgatac gctgcgcctg ccaatgacac caatcaccga cagtggtcgt
    30541 gagacggtca gagcggcgct taagcatgcc ggtttgctgt aaagtttagg gagatttgat
    30601 ggcttactct gttcaaaagt cgcgcctggc aaagattgcg ggtgtttcgc ttgttttatt
    30661 actcgctgcc tgtagttctg actcacgcta taagcgtcag gtcagtggtg atgaagccta
    30721 cctggaagcg gcaccgcttg cggagcttca tgccccggct ggaatgattt tgccggtgac
    30781 ctccggtgat tatgcaatcc cggtgaccaa cggtagtggt gctgtcggta aggcgctgga
    30841 cattcgtcca ccagcccagc cgctggcact ggtttctggc gcgcgtaccc agttcacggg
    30901 cgataccgct tcattgctgg tggaaaatgg tcgtggcaat actctgtggc cgcaggtggt
    30961 tagcgtgctg caggcgaaaa actacaccat cacccaacgt gatgatgctg gtcagacact
    31021 gaccaccgat tgggtacaat ggaaccgtct ggacgaagac gagcagtatc gtggtcgtta
    31081 tcaaatctct gttaagccgc agggttatca gcaggcggtt acggttaaac tgctgaacct
    31141 ggaacaggcg ggcaaaccgg ttgcagacgc ggcttccatg cagcgttaca gcacggagat
    31201 gatgaacgtt atttccgccg gtctggataa atctgccact gacgccgcga acgctgcgca
    31261 aaatcgtgcc tccaccacta tggacgtaca aagtgcagct gatgacaccg gtttaccaat
    31321 gctggtcgta cgcgggccgt tcaatgtggt ttggcaacgt ctgccagcgg cgctggaaaa
    31381 agtgggcatg aaagtgaccg acagcacccg ttcgcagggc aacatggccg taacttataa
    31441 gccgctgtct gacagcgact ggcaggaact gggcgcgagc gatccaggcc tggcatccgg
    31501 tgactataaa ctgcaggttg gcgatttaga taaccgcagc agcctacagt tcatcgatcc
    31561 gaaaggtcat actctgactc agagtcagaa cgacgcgctg gtagctgtct tccaggctgc
    31621 gtttagcaag taaaaataca gggctggaat catccggccc ttttttctga tatgatacgc
    31681 aaacgtgtgc gtcggcagga aaacgcgatt ttagcggtaa ttcgcacgaa atttgtttgt
    31741 cggacgtagt tcggataagg cgttcacgcc gcatccgaca aaacatccgg cacaccagac
    31801 agcaaaagat tttaaaacgt taattcacac ccaggagtga taaagatgca aaagcaagct
    31861 gagttgtatc gtggtaaagc gaaaaccgta tacagcacgg aaaacccgga cctgttggtg
    31921 ctcgaattcc gcaatgatac gtcagcaggg gatggcgcgc gcattgagca gtttgatcgc
    31981 aaaggtatgg tgaacaacaa gttcaattac ttcattatga gcaaactggc tgaagcgggt
    32041 atcccgactc aaatggagcg tctgctctcc gataccgaat gtctggtgaa aaagctggat
    32101 atggtgccgg ttgagtgtgt cgtgcgtaac cgtgctgctg gctctctggt gaaacgtctt
    32161 ggaatcgaag aaggtattga gctgaacccg ccgctgttcg atctgttcct gaaaaacgac
    32221 gccatgcacg atccgatggt caacgaatct tactgcgaaa cctttggctg ggtgagcaaa
    32281 gagaacctgg cgcgtatgaa agagctgacc tacaaagcga acgacgtgct gaaaaaactg
    32341 ttcgatgatg ctggtctgat tctggtcgac ttcaagctgg agtttggcct gtacaaaggt
    32401 gaagtggtac tgggcgatga gttctccccg gacggcagcc gcctgtggga caaagaaaca
    32461 ctggagaaaa tggacaaaga ccgtttccgc cagagcctcg gtggcctgat cgaagcctat
    32521 gaagccgtcg cccgccgcct gggtgtacag ctggactgat ttttctggtt gcttgtttct
    32581 ttagccggag ggtactccgg ctaaatgtct ttgatacccc cgctacaata cttctcatta
    32641 tctccctttg acaggaaagt cataccaggc caatatgtat atacataacg tatatcccaa
    32701 aaaggggggg gctatggaaa ctactgtatt tttaagtaat cgcagtcagg ccgtcagatt
    32761 accgaaagcg gtcgctctgc cagaagacgt taagaaagta gaaattattg cgattggtcg
    32821 tacaagaatc atcactcccg ccggtgagtc ctgggacagt tggtttgatg gcgaaaatgt
    32881 gagtgcggat tttatggata tccgtgatca acccgcaatg caggaaaggg aatcattttg
    32941 atgctgaaat tcatgctgga taccaatatc tgtattttca ctattaagaa taaaccagta
    33001 catgtcagag agcggtttaa cctgaattct ggccggatgt gtataagttc tgtaacattg
    33061 atggagctta tctatggggc agaaaaaagt cagatgccag aacgcaattt ggcggttatt
    33121 gaaggtttcg tatctcgttt ggtagtgctt gattatgata gtgctgctgc gacgcatacc
    33181 gggcaaatca gggccgagct tgcccggcag ggaaggcctg ttggaccttt tgaccagatg
    33241 atagcgggac atgctcgcag tcgcggtttg attgtggtta ccaacaatac ccgagagttt
    33301 gagcgagtgg cgggaatcag actagaagac tggagctaac attctctttt acttctaaca
    33361 ttcgtaaatt atataacgtg gcatgaagcc ttaattcctc cgccatttca tcccgctttc
    33421 ctgtggtaat cttcctctga accacctacg atcatttcta taatgaatat attgttgagg
    33481 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct
    33541 ccggtggtcc gtctatgggc ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggctga
    33601 ttttactgtt agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga
    33661 ctggacagcc ggtttcccaa caacaatcaa cgcggtccat tagccctaat gaagacgaag
    33721 ctgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg
    33781 agaagatggg taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca
    33841 ccggctgcgg ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag
    33901 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
    33961 ttgcccaggg gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca
    34021 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat
    34081 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat agtatgcagc
    34141 agcaaggcgt tctggaaacc ggcgatctgg aagaggcgct gaacgcggcg caggccatcg
    34201 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagatagt ttcacacatg
    34261 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
    34321 aatgcaatac ttttggtaaa agcatttaac ttccggggca gggatggctg aactgactgc
    34381 gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
    34441 cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga
    34501 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca
    34561 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
    34621 cgctgccttt gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact
    34681 ccctgtatgg gaagagtggg aaaaccaacc tgatgccgat tcgctgcgct ggagtgattg
    34741 ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga
    34801 taacgatgct atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
    34861 tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca
    34921 gctactgacc atgcctccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc
    34981 ggcgttggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc
    35041 aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcacc
    35101 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc
    35161 catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac
    35221 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
    35281 tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct
    35341 ggaaaaaatg gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca
    35401 aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc
    35461 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg
    35521 ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa acgagattgc
    35581 cggagcgctg tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg
    35641 ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag
    35701 caatccactg gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc
    35761 gcgtcagcgg gaaggcacag ggcagcaact tattgctggt gctttgcaat atacgcgtga
    35821 cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaccg
    35881 ctgcggtttt gtgctggtgc ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac
    35941 ggcgatggcg ctgttaccga tgagtaatgc gggtaaacaa ctggctgaac gtgagcatta
    36001 ccgtttacgt cgcgatgcgc aagctctcgc aaagtggaat ggcgaaacgc ttcctgttga
    36061 tccactaaac gatgccgtcc tttctgacga cgactggctt gaactggccg gttttgcttt
    36121 cgctcatcgt ccgctattaa catcgttagg ttgcttattg cgtctgctac aaaccagtga
    36181 actggcatta ccggcgctgc gtgggcgttt acagaaaaac gtcagcgacg cgcagttatg
    36241 taccacactt aaactttcag gccgcaagat gttactggtc cgtcagcggg aagaggccgc
    36301 gcaggcgctg ttcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca
    36361 atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga gtcagcggcg
    36421 taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa
    36481 gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg gataacccgg
    36541 tggcaatggg ggaaataggt agctggtttg caccgctgtt tcctgatgcg ctggtggtga
    36601 gtgtcggtgg cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcagtgcagg
    36661 gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg tttattgaaa
    36721 cggtacgcta ctggcagaaa cagagcggag taggggctaa cgccacggcg ctaattggtt
    36781 tctcacaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc cttgcttcgc
    36841 gcgtcattgc ctttaacgga cgctatgcga gcctgccgga gaccgcgtct acagccacga
    36901 caattcactt gattcatggt ggtgaagatc cggttatcga tctggcgcat gcagttgcgg
    36961 cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag
    37021 gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc
    37081 cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg
    37141 aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg catcaggcat
    37201 cgttcaccat tgccggatgt ggcgttaacg ccttatccgg cctacggttg agcactcagc
    37261 ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa
    37321 atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt cgacgcgctt
    37381 gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca gaatccacca
    37441 atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc aggcgacgag
    37501 ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag cgttcacaca
    37561 ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct gcgcccccat
    37621 gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg gtttaatttc
    37681 attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa
    37741 tggctgcccg gaaagccacc agtccaccgt ataacgcagt tgatgttttt caagcagggc
    37801 aagcacctgt gctttgatca tctcatcagt cagttcggtg ctgaagcgga agttaaactg
    37861 cacaaacagt tcacccggaa taacattgtt actgcccgtt cccgcctgaa tattagcaat
    37921 ctgcatactg gtcgccggga aaaattcatt gccctgatcc cactcaatag ccactaattc
    37981 attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgcgggt aggccacatg
    38041 cccctgaacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac catttttcac
    38101 cacatcacca accacttcga tactcgacgg ttcgccgacc aggcagtaat cgagacgctc
    38161 attacgtgcc atcaacgctt cgacgacttt taccgtaccg ttgtgggcac tggcttcttc
    38221 atcagaggtg atcagaaatg ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg
    38281 ttctgccgct accaccatcg ccgccagcga gcctttcata tctgccgcac cgcgtccgaa
    38341 taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat cggcgtcgcc
    38401 aggcggcacc acgtcggtat gcccggcaaa ggctaacgtt tcaccttgcc cacgccatgc
    38461 ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa
    38521 acgttcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat
    38581 aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat ggaaaaattg
    38641 ctgataactg gaatcactga aacccagcag cataggctta ccaggcgcgc agagcaatgg
    38701 acgtttgata attgcaggca tttcagtcat taatgccgcc gcagaggccg catcggtgat
    38761 tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt tcagtaacgc
    38821 ttcccagcct aattcgttga taaaaccgtt caataattcg ctgtccagcc catcgacgcg
    38881 gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag cctttttaat
    38941 ggtgtcacaa tttttgatgc cgtaaagtgt aaccataatg aatccttttt cgatttatga
    39001 gaataaatac t
//
LOCUS       DAZFWP020000027        38426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFWP020000027 DAZFWP020000000
VERSION     DAZFWP020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.027"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            148..1806
                     /gene="ybjD"
                     /locus_tag="ACHK0Z_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     CDS             148..1806
                     /gene="ybjD"
                     /locus_tag="ACHK0Z_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU4501321.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1803..2759)
                     /gene="ybjX"
                     /locus_tag="ACHK0Z_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             complement(1803..2759)
                     /gene="ybjX"
                     /locus_tag="ACHK0Z_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU4501322.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2910..4025
                     /gene="macA"
                     /locus_tag="ACHK0Z_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             2910..4025
                     /gene="macA"
                     /locus_tag="ACHK0Z_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU4501323.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4022..5968
                     /gene="macB"
                     /locus_tag="ACHK0Z_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             4022..5968
                     /gene="macB"
                     /locus_tag="ACHK0Z_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU4501324.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAETEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVIERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6041..6265)
                     /gene="cspD"
                     /locus_tag="ACHK0Z_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             complement(6041..6265)
                     /gene="cspD"
                     /locus_tag="ACHK0Z_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU4501325.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6415..6489
                     /gene="yljB"
                     /locus_tag="ACHK0Z_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             6415..6489
                     /gene="yljB"
                     /locus_tag="ACHK0Z_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU4501326.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6588..6908
                     /gene="clpS"
                     /locus_tag="ACHK0Z_RS06375"
                     /old_locus_tag="pgaptmp_001275"
     CDS             6588..6908
                     /gene="clpS"
                     /locus_tag="ACHK0Z_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU4501327.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6939..9215
                     /gene="clpA"
                     /locus_tag="ACHK0Z_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             6939..9215
                     /gene="clpA"
                     /locus_tag="ACHK0Z_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU4501328.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9559..9646)
                     /locus_tag="ACHK0Z_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     tRNA            complement(9559..9646)
                     /locus_tag="ACHK0Z_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9610..9612),aa:Ser,seq:gga)
     gene            complement(9900..10118)
                     /gene="infA"
                     /locus_tag="ACHK0Z_RS06390"
                     /old_locus_tag="pgaptmp_001278"
     CDS             complement(9900..10118)
                     /gene="infA"
                     /locus_tag="ACHK0Z_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU4501329.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10403..11107)
                     /gene="aat"
                     /locus_tag="ACHK0Z_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             complement(10403..11107)
                     /gene="aat"
                     /locus_tag="ACHK0Z_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU4501330.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCSESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11149..12870)
                     /gene="cydC"
                     /locus_tag="ACHK0Z_RS06400"
                     /old_locus_tag="pgaptmp_001280"
     CDS             complement(11149..12870)
                     /gene="cydC"
                     /locus_tag="ACHK0Z_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU4501331.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12871..14637)
                     /gene="cydD"
                     /locus_tag="ACHK0Z_RS06405"
                     /old_locus_tag="pgaptmp_001281"
     CDS             complement(12871..14637)
                     /gene="cydD"
                     /locus_tag="ACHK0Z_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU4501332.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14760..15725)
                     /gene="trxB"
                     /locus_tag="ACHK0Z_RS06410"
                     /old_locus_tag="pgaptmp_001282"
     CDS             complement(14760..15725)
                     /gene="trxB"
                     /locus_tag="ACHK0Z_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU4501333.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16270..16764
                     /gene="lrp"
                     /locus_tag="ACHK0Z_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             16270..16764
                     /gene="lrp"
                     /locus_tag="ACHK0Z_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU4501334.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16899..20966
                     /gene="ftsK"
                     /locus_tag="ACHK0Z_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             16899..20966
                     /gene="ftsK"
                     /locus_tag="ACHK0Z_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU4501335.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21121..21732
                     /gene="lolA"
                     /locus_tag="ACHK0Z_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             21121..21732
                     /gene="lolA"
                     /locus_tag="ACHK0Z_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU4501336.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21743..23086
                     /gene="rarA"
                     /locus_tag="ACHK0Z_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /gene_synonym="mgsA"
     CDS             21743..23086
                     /gene="rarA"
                     /locus_tag="ACHK0Z_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU4501337.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23177..24469
                     /gene="serS"
                     /locus_tag="ACHK0Z_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             23177..24469
                     /gene="serS"
                     /locus_tag="ACHK0Z_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU4501338.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24708..27152
                     /gene="dmsA"
                     /locus_tag="ACHK0Z_RS06440"
                     /old_locus_tag="pgaptmp_001288"
     CDS             24708..27152
                     /gene="dmsA"
                     /locus_tag="ACHK0Z_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU4501339.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27163..27780
                     /gene="dmsB"
                     /locus_tag="ACHK0Z_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             27163..27780
                     /gene="dmsB"
                     /locus_tag="ACHK0Z_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU4501340.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27782..28645
                     /gene="dmsC"
                     /locus_tag="ACHK0Z_RS06450"
                     /old_locus_tag="pgaptmp_001290"
     CDS             27782..28645
                     /gene="dmsC"
                     /locus_tag="ACHK0Z_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU4501341.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28681..29307)
                     /gene="ycaC"
                     /locus_tag="ACHK0Z_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     CDS             complement(28681..29307)
                     /gene="ycaC"
                     /locus_tag="ACHK0Z_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU4501342.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29622..30770
                     /gene="ycaD"
                     /locus_tag="ACHK0Z_RS06460"
                     /old_locus_tag="pgaptmp_001292"
     CDS             29622..30770
                     /gene="ycaD"
                     /locus_tag="ACHK0Z_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4501343.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30980..32410
                     /gene="ycaM"
                     /locus_tag="ACHK0Z_RS06465"
                     /old_locus_tag="pgaptmp_001293"
     CDS             30980..32410
                     /gene="ycaM"
                     /locus_tag="ACHK0Z_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4501344.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32411..33319)
                     /gene="ycaN"
                     /locus_tag="ACHK0Z_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             complement(32411..33319)
                     /gene="ycaN"
                     /locus_tag="ACHK0Z_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4501345.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            33419..34009
                     /gene="ycaK"
                     /locus_tag="ACHK0Z_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     CDS             33419..34009
                     /gene="ycaK"
                     /locus_tag="ACHK0Z_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HGU4501346.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(34091..34831)
                     /gene="pflA"
                     /locus_tag="ACHK0Z_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     CDS             complement(34091..34831)
                     /gene="pflA"
                     /locus_tag="ACHK0Z_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU4501347.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(35023..37305)
                     /gene="pflB"
                     /locus_tag="ACHK0Z_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     CDS             complement(35023..37305)
                     /gene="pflB"
                     /locus_tag="ACHK0Z_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4501348.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37360..38217)
                     /gene="focA"
                     /locus_tag="ACHK0Z_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     CDS             complement(37360..38217)
                     /gene="focA"
                     /locus_tag="ACHK0Z_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU4501349.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9485 a         9357 c        10202 g         9382 t
ORIGIN      
        1 acttatcagc aagagggagt ataacgcgat tattcgctca tttttcagac atttgccatg
       61 cttaaatgtg atgtcatcac gtattagcaa ggcctttccc gttatactgc cagcgtaaag
      121 gataagtcac atatttctgg aggggatatg attcttgagc gcgttgaaat tgtgggtttt
      181 cgcggtatca accgtttgtc gttgatgctg gaacaaaaca acgtcctgat tggggagaac
      241 gcgtggggta aatccagctt gctggacgcc ttaaccctgc tgctatcgcc agaatcagat
      301 ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa
      361 catcatctgc atattatttt gaccttccgc gaatcgctgc caggccgaca tcgggttcgc
      421 cgttatcggc cgctggaagc gtgctggacg ccatgcaccg atggctatca ccgtattttt
      481 tatcgtctgg aaggggagag tgcggaagac ggcagcgtga tgacactgcg cagttttctc
      541 gataaagacg gacatccgat tgatgtcgag gatattaacg atcaggcacg ccatctggtg
      601 cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc
      661 acggtgccaa atgtccccaa tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt
      721 gagttgtcct cacatccgca aaatctctct gatggacaga ttcgtcaggg actttccgca
      781 atggtgcagt tgcttgagca ttatttctct gagcaggggg ctggacaggc gcgatatcgt
      841 ttaatgcggc ggcgggccag caatgagcaa cgaagctggc gctatctgga tatcatcaac
      901 cggatgattg accgacctgg agggcgctcg tatcgggtta ttttgctcgg cctgtttgca
      961 actttattgc aggcaaaagg cacattgcga ctggataaag acgcccgtcc attgttgctg
     1021 attgaagatc cagaaacccg tttacacccc attatgcttt cagttgcctg gcatctgttg
     1081 aatcttctgc cattgcagcg cattgccacc accaactcgg gtgagttgct ttcgttaact
     1141 ccagtggaac atgtttgccg cctggtgcgt gaatcttctc gtgttgccgc ctggcgtctg
     1201 gggccaagtg gcttgagcac cgaagacagc cgccgcatct cttttcacat tcgttttaat
     1261 cgtccgtcat cgctgtttgc tcgctgctgg ttgctggtgg aaggggaaac agaaacctgg
     1321 gttatcaacg aactggcgcg ccagtgtgga caccatttcg atgccgaagg gatcaaggtc
     1381 attgagtttg cccagtccgg gctaaagcca ctggttaaat ttgcccgccg aatggggatt
     1441 gaatggcatg tactggtcga tggcgatgaa gcagggaaga aatatgccgc tacggtacgc
     1501 agcctgttga ataacgatcg ggaagccgaa cgagaacatt taacggcgtt accggcgctg
     1561 gatatggaac attttatgta tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa
     1621 atcccggaaa atgtaccgat gaatctgcgc aaaattatct cgaaagcgat ccatcgctct
     1681 tccaaacccg atcttgccat tgaagtggca atggaggccg gacgtcgtgg tgtggactcc
     1741 gtaccgacgc tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg
     1801 gattaaccgc gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc
     1861 cgacggtatt cagcccgttt tttactggcg atttcggcaa tctcttttcg tgctatctgt
     1921 gctggaaggc ggtaatggcg ttcagcatca catacgccgc caaccgattc ccagaaagcg
     1981 ttgtaatcgg catggatctt gccttcttta tcacgataac gcaggctgcg gtaaatatgc
     2041 gtttcattgc tgacggcaat aatctgctct acctgcaaac gttgggcaaa cagacaggcc
     2101 gcttccatca cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc attctggatt
     2161 tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc caataaacat cgttcttttc
     2221 ccctgatatt cacacagggt aaacgtgatc tctgccagag gaataccttc gctgttgcga
     2281 aacaggattg tgctgtcacc ttctttatcc attgagatca tcatggtcag ctcaagcgtg
     2341 aactgctcgc cgtttttgcc ttccagcttc gccagttgca gcccgggggt attcaaatat
     2401 aagctgaatt cttccgccga catacatccg cggagtaacg cataatggta acgtaacgcc
     2461 tccagcaatt gcttacggct aagattcgcc gcaaggtaag ggcgatgcag acgcacaggc
     2521 agtcgcggtt ggcgcgttaa caacacattg agattaggcc agtgggaaag ttcgttcatc
     2581 cactcaacgc ttaaacgcgg cataatcaac gagcgcagca aaaatttctg gcgaaaacta
     2641 cggcgatgcc agaatttacc cggccgacac tgtccacgtg ccagactaag aaaaagtgac
     2701 aggctgctga gagattcaga tggcgtaaag gtccgttcag ttagctgcga catattcatg
     2761 aaatcaatgg ttatacatga cgtcgatttc accattgcgt atcttaacca aacatcaata
     2821 gtgtgattac taacgtaaat tttagggttt tgttgatatt tcgttgaagt taatgacccg
     2881 gattggcata tggagtattc agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt
     2941 acgttattgc gctggtgata gtcatcgccg gactgattac gttatggaga attcttaacg
     3001 cacccgtgcc gacttatcag acactgattg tgcgccccgg tgatttacag caaagcgtgc
     3061 tggcgaccgg aaagctggac gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc
     3121 agttgaaaac tctgtcggtg gcgattggcg ataaagtaaa aaaagaccag cttttagggg
     3181 ttattgatcc tgaacaggct gaaaaccaga ttaaggaggt cgaagcaacg ctgatggagc
     3241 tacgtgcgca gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg acgtattccc
     3301 gtcagcaacg tctggcacaa acgcaggcgg tttcacagca ggatctcgac accgccgcga
     3361 cggagatggc tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc
     3421 aggcttctct cgatacggct aaaaccaatc tcgattacac tcgcatcgtt gccccgatgg
     3481 ccggggaagt cacgcaaatc accactctgc aaggccagac ggtgattgcc gcacaacaag
     3541 caccgaacat tctgacgctg gcagatatga gcaccatgct ggtaaaagcg caggtttctg
     3601 aagcggatgt aatccacctg aagccggggc aaaaagcctg gtttacggtg cttggcgatc
     3661 cactgacgcg ctacgagggg caaatcaagg atgtactacc gacgccggaa aaggttaacg
     3721 acgctatttt ctattacgcc cgttttgaag tccccaaccc caatggtttg ctgcggctgg
     3781 atatgactgc gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg acgatccctc
     3841 tgtcggcgtt aggcgatccg gttggcgata atcgttataa agtcaaattg ttgcgtaatg
     3901 gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat gttgagattg
     3961 tcaaagggct tgaagcgggc gatgaagtgg tgattggtga ggccaaacca ggagctgcac
     4021 aatgacgcct ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg gtgatgagca
     4081 ggttgaggtg ctgaagggca tcaccctcga tatttatgcc ggtgagatgg tagcgattgt
     4141 tggcgcttca ggttccggta aatcgaccct gatgaatatt ctcggctgtc tggataaggc
     4201 caccagcggc acctatcgcg tcgccggtca ggatgttgcc acactggacg ccgatgcgct
     4261 ggcgcaactg cgccgcgagc atttcggctt tattttccag cgttaccatt tactttcgca
     4321 tttaaccgcc gagcagaacg ttgaagtacc cgccgtctat gccggtcttg agcggaaaca
     4381 gcggctgctt cgcgcccagg ggttactgca acgactgggg ctggaagacc gtacagagta
     4441 ttatccggca cagctttcgg gtggtcagca acagcgcgtc agcatcgcgc gggcattgat
     4501 gaacggcggt caggtaattc ttgccgatga accaaccggc gcactggaca gccattctgg
     4561 cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt
     4621 cacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga
     4681 aattgtgcgc aatcctcccg ccattgaaaa agtgaatgtt gctggcggga cggagcctgt
     4741 tgtcaacacg gtgtctggct ggcggcagtt tgtcagcggt tttaacgagg cgctgacgat
     4801 ggcatggcgg gcgctggcag cgaataaaat gcgtacttta ctgaccatgc tggggattat
     4861 tatcggtatt gcgtcggtgg tttccattgt cgtggtgggt gacgccgcca aacaaatggt
     4921 gctggcggat attcgttcta ttggtacgaa tactattgat gtctatcccg ggaaagattt
     4981 tggcgatgac gatccgcaat atcagcaggc gctgaagtac gacgacttaa tcgccatcca
     5041 aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg caaaacctgc gcctgcgtta
     5101 taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta
     5161 tggcatgacc ttcagtgaag gaaacacctt taatcaggag cagctgaacg gtcgtgcgca
     5221 ggtcgtggtt ctcgacagta atactcgccg ccagcttttc ccccataaag cagatgtggt
     5281 tggcgaggtg attctggtcg gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa
     5341 acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc
     5401 cgggcgagtt atgggccagt cgtggctgaa ctccattact gtcagggtaa aagaaggatt
     5461 tgacagcgcc gagacggaac agcaactcac gcgtttactt tcactgcgcc acggaaagaa
     5521 ggatttcttt acctggaaca tggacggcgt cttgaaaact gttgaaaaga ccacacgtac
     5581 tttacaactg tttctgacgc tggtggcggt gatttcgctg gtggtgggcg gtattggtgt
     5641 aatgaatatt atgctggtgt cagtgatcga gcggacgcgg gaaattggca ttcgcatggc
     5701 tgtaggtgcg cgagcaagcg atgttttgca acagttcctg atcgaagccg tactggtttg
     5761 cctggtcggt ggcgcgttgg gaataactct gtcactgtta attgctttca ccttgcagct
     5821 tttcttaccc ggctgggaga ttggtttttc accgttggca ctgctgctgg cgtttctctg
     5881 ctcgacggcc accgggattt tatttggatg gttacccgca cgaaatgcgg cacgactgga
     5941 tccagtagat gctctggcac gagagtaatt tttgagataa aaatgccagc cgatcgggct
     6001 ggcattttgc ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta
     6061 cttcgacggg cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact
     6121 gaacggattg tccagctttt agcgttctgt aaccatccat ctgaatggtg gaataatgag
     6181 cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga
     6241 accacttaac agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag
     6301 atggaataaa ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca
     6361 atttagagaa ttagggcgag ccgtcaagcg tttgacaggg gccagggggc aggtatgaat
     6421 caaaaatttg aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac
     6481 gatagatgat agttatctat catgtggagt agattggtca ggcaaataag ctcttgtcag
     6541 cggcagggcg ttctgccgat aaccgtaacc gaagatgata actgacaatg ggtaaaacga
     6601 acgactggct ggactttgat caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc
     6661 catctatgta taaagtgata ttagtcaatg atgattacac tccgatggag tttgttattg
     6721 acgtgttaca aaaattcttt tcttatgatg tagaacgtgc aacgcaattg atgctcgctg
     6781 ttcactacca ggggaaggcc atttgcggag tctttaccgc cgaggttgca gaaaccaaag
     6841 tggcgatggt gaacaagtac gcgagggaga atgagcatcc attgctgtgt acgctagaaa
     6901 aagcctgaat gcaggcataa aaattggggg aggtgcctat gctcaatcaa gaactggaac
     6961 tcagtttaaa tatggctttc gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg
     7021 agcacttgtt actggcgctg ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt
     7081 ctgtggattt ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa accacacccg
     7141 ttctgcctgc cagtgaagag gagcgcgaca cacagccgac gctgagtttt cagcgtgtac
     7201 tgcaacgtgc ggtcttccat gtccaatcct ccggtcgcaa tgaggtaacc ggtgcaaacg
     7261 ttctggtcgc tatctttagc gaacaggagt cgcaggcggc atatctgttg cgtaaacatg
     7321 aagtcagccg tctcgatgtg gtgaacttta tctctcatgg cacgcgtaaa gacgagccga
     7381 cacagtcttc tgatcctggc agccagccaa acagcgaaga acaagctggt ggggaggaac
     7441 gtatggagaa tttcacgacg aacctgaatc agcttgcgcg cgtgggcgga atcgacccac
     7501 tgattggtcg tgagaaggag ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa
     7561 acaacccgct gctggtgggg gaatctggtg tcggtaaaac cgcgattgcg gaaggtcttg
     7621 cctggcgaat tgttcagggc gatgtgccgg aagtgatggc tgactgtacg atttactctc
     7681 tcgatatcgg ttctctgtta gcgggcacta aatatcgcgg cgactttgaa aaacgtttta
     7741 aagcgttgct caagcagctg gagcaggaca ctaacagcat cctgtttatt gatgagatcc
     7801 acaccattat cggtgcgggg gcagcgtctg gtggccaggt cgatgcggct aacctgatca
     7861 aaccgttgct ctccagcggt aaaattcgcg taattggttc gacaacctat caggagttca
     7921 gcaacatttt cgagaaagac cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg
     7981 aaccgtcgat cgaagaaact gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc
     8041 accacgacgt gcgttatacc gcaaaagcgg tgcgtgcagc ggtagagctg gcggtgaaat
     8101 acattaacga tcgtcatctg ccggataaag ccattgacgt tatcgacgaa gcgggcgctc
     8161 gcgcacgcct gatgccggta agcaaacgca agaaaaccgt taatgtggcg gatattgagt
     8221 ccgtggtggc ccgtattgcg cgcattccag agaagagtgt ttctcagagt gaccgcgata
     8281 ccctgaaaaa cctcggcgat cgcctgaaaa tgctggtctt cggtcaggat aaagccattg
     8341 aggcgctgac tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg
     8401 ttggttcgtt cctgtttgcc ggccctaccg gggtcgggaa aacagaggtg acggtacagc
     8461 tttcgaaagc gttgggcatt gagctgctgc gctttgatat gtccgagtat atggaacgcc
     8521 ataccgtcag ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat cagggaggtt
     8581 tgctgactga tgcggtcatc aagcatccac atgcggtgct gttgctggac gaaatcgaga
     8641 aagcgcatcc ggacgtgttc aatattctgt tgcaggtgat ggacaacggt acgctgaccg
     8701 ataacaacgg acgcaaagcg gacttccgta acgtggtgct ggtgatgacc accaacgccg
     8761 gggtacgtga aactgagcgt aaatccattg gtcttatcca ccaggataac agcaccgatg
     8821 cgatggagga gatcaagaag atctttacac cggaattccg taaccgtctc gacaacatta
     8881 tctggtttga tcatctgtca accgacgtga tccatcaggt ggtggataaa ttcatcgtcg
     8941 agttgcaggt tcagctggat cagaaaggtg tttctctgga agtgagccag gaagcgcgta
     9001 actggctggc cgagaaaggt tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca
     9061 tccaggacaa cctgaaaaaa ccgctcgcca acgaactgct gtttggttcg ctggtggacg
     9121 gcggtcaggt caccgtcgcg ctggataaag agaaaaatga gctgacttac ggattccaga
     9181 gtgcacaaaa gcacaaggcg gaagcagcgc attaatctga ttgttaggta ggttggtcaa
     9241 gtccgtaatc tcgaaagagg ttacggactt tttgtttatg gggtggagga ggttcagacc
     9301 ctttttttaa tgatgatggt aagttgttga taattagtgc tgcgggaagg taaggataaa
     9361 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9421 tatttgagtt cactgccata caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9481 cgaacgcgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9541 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9601 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgtcg
     9661 cctgacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
     9721 agaattttaa tgataattat tgtttgctca tactgtaaac aagttgtgca gtatatctac
     9781 atcgagacaa gttacggact tatacttcca aagtacttca tacatatcac aaaataaaaa
     9841 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
     9901 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
     9961 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10021 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10081 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10141 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10201 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10261 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10321 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10381 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcatcgt ggtacccaga
    10441 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10501 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10561 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10621 ccatccggct gaacatggac tcgctacaaa atagcgttcc ctgggccacg ccgtacatac
    10681 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatggccg agttcgtgaa
    10741 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    10801 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    10861 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    10921 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    10981 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11041 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11101 ggcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11161 cttgaactgg taataacgcc cctgtctggc aagcagctct gcgtgagtac cttgctcaat
    11221 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11281 ggtgaccatt aacaccgttt tctcacgcat catttctgca agcaattcaa ggatctggct
    11341 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11401 taacagcgca cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11461 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccaggc caacgcgacg
    11521 caagctctcc gccagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11581 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11641 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    11701 ccaagcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    11761 cgcaatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    11821 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    11881 ttgggtatca gggaaggtga cttccggttt ttgatccgtt aaatcagtga tccgaacggc
    11941 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12001 cgcggctaac gcgcagaaga caaacagggc aattaacgcg ccgggttgag cattgccgcc
    12061 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagtagcat
    12121 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12181 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12241 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12301 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12361 cataatgccg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12421 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcagat aaagatgatc
    12481 gagcgtatca acatccgcca ccacgcgatt gagcaattcg ccctgacgat agcgcgccag
    12541 tccggcaggg gagaggggca gcaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12601 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12661 tgctgcgcca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    12721 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    12781 gagcagcgtg acaattgcca gcacgatacc aagacttaac atccatttat gacgtttata
    12841 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    12901 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    12961 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13021 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13081 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13141 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13201 aacaggcgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13261 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13321 caatgttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13381 ttctggtgat aaatcgcgta attctatccc gttgattcgt aatgatccct gatatgaaag
    13441 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcgac caaccaacac
    13501 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13561 cgacgtgata aacagatcct cggcctcaat ggtcaccgga tcggtcgatg ctaattccgc
    13621 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13681 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgcaatg gctggaaaaa
    13741 ctctggcgca aggatcaggg ccagaaaacc cgcagccagc gtcacaccgg tatcgtagtg
    13801 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    13861 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagccgta gcacttccat
    13921 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    13981 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14041 agcaagagcg agaaagttac gccggttagc atcggcagcc cccatcccaa ccagcgccat
    14101 aaacaacgga attaacggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14161 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcaggta
    14221 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accaacgtcg cccagctccc
    14281 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14341 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14401 cacccatgcg cgcagtacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14461 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14521 aataatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14581 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgggatt tattcattgc
    14641 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    14701 aaaggcgacc catagtcgca tggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    14761 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcct
    14821 gtaccggccg aagtaatggc ctgacgataa atatgatcca tcacgtcacc cgcggcaaag
    14881 acgccaggga tgctggtctg ggtggcatta ccatgaatac ccgactgcac tttgatgtag
    14941 ccgttttcca gttccagctg cccttcgaaa atggcagtat tcgggctgtg accgatagca
    15001 acaaacagac cagcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15061 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15121 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15181 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15241 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15301 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15361 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15421 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15481 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgctccat taataacgga
    15541 ccggtcagat cgtttggatc gccaggccag ttttccactt ccgtggtggt ggtcagttgg
    15601 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15661 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    15721 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    15781 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    15841 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    15901 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    15961 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaaacagta
    16021 ttcttatatg cgcataacca tgcatgtaaa taccatgttt accgtgctag tgaaatctac
    16081 gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct
    16141 caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg
    16201 tagcgactct gaacagtgat gtttcagggt cagacaggag tagggaagga atacagagag
    16261 acaataataa tggtagatag caagaagcgc cctggcaaag atctcgaccg tatcgatcgt
    16321 aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt
    16381 gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt
    16441 attcagggct atacggcgct gcttaacccc cattatctgg atgcatcact tctggtattc
    16501 gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta
    16561 caaaaacttg aagaaattca ggagtgtcat ttagtatctg gtgatttcga ctacctgttg
    16621 aaaacacgcg tgccggatat gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt
    16681 ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat
    16741 cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac
    16801 actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag
    16861 catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag
    16921 acaaagaagt cacattgaca aagttaagta gcggccgtcg ccttctggaa gcgttgctga
    16981 tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg
    17041 accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg
    17101 gtgcgtggct ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg
    17161 tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtccagtgac gaatacattg
    17221 attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg
    17281 gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca
    17341 gcttactaag cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc
    17401 tctgcgtttg ggctgcgggc ctgacgctgt tcaccggttg gtcatgggtg accattgctg
    17461 aaaaactcgg cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg
    17521 atgatacctg ggtcgatgaa gatgaatatg aagatgacga agagtatgaa gatgaaaacc
    17581 acggcaaaca gcatgaatcg cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta
    17641 aacggttggc ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct
    17701 ccggtaagcg gatggatgat gacgaagaga ttatctacac tgcacgcggt gtggctgccg
    17761 acccggatga cgtcctattt tcgggcaatc gtgcaacgca gccagaatat gacgaatacg
    17821 atccattatt aaacggtgcg ccaattaccg aacctgtcgc tgtagcagct gctgctacca
    17881 cggcgacaca aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct
    17941 ctgttgatgt tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa
    18001 cgggagagcc ggttattgct cctgcaccgg aaggttaccc acagcagtca caatatgcgc
    18061 agcctgcagt gcaatataat gagccgctgc aacaaccagt acagccgcag cagccgtatt
    18121 atgcacctgc agctgaacaa cctgcgcaac aaccttacta cgctcctgcg gcggaacaac
    18181 ctgttcagca accgtattac gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc
    18241 aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact gagcaaactt
    18301 atcagcaacc agccgctcag gagccgttgt accaacagcc gcaatccgtt gaacagcagc
    18361 ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccgctttact
    18421 attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc
    18481 aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgttg aaagcgcctt
    18541 ctgttgcagc agtacctcca gtagaagccg ctgccgctgt ttctccgctg gcatctggcg
    18601 tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc
    18661 tggcaaatag cggtggaccg cgtccgcaag tcaaagaggg gattggcccg cagttgccac
    18721 gaccgaaacg tatccgcgtg ccaactcgtc gtgaactggc gtcttacggt attaagctgc
    18781 cctcacagcg tgcggcggaa gagaaagccc gtgaagccca gcgcaatcag tatgattctg
    18841 gcgatcagta taacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt
    18901 tcgcccagac acagcagcaa cgctatggcg aacagtatca acatgatgtg cccgtaaacg
    18961 cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg caaactcaac
    19021 aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg
    19081 aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgttg tttacgccaa
    19141 ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc gcagcagcag tatcagcagc
    19201 cgcaacaacc agttccgcca cagcagcaat atcagcagcc gcaacagccg gttgcgccgc
    19261 agccgcagta tcagcagcca caacagcagg ttgcgccgca gccgcagtat cagcagccac
    19321 aacaaccggt tgcgccacag ccgcaatatc agcagccgca acaaccagtt gcgccgcagc
    19381 cgcagtatca acagccgcaa caaccggttg caccgcagca gcaggatacc ctgcttcatc
    19441 cgctgttgat gcgtaatggc gacagccgtc cgttgcataa accgacgacg ccgctgcctt
    19501 ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg
    19561 aacaaatggc tcgcctggtg gaagcgcgtc tggctgattt ccgtattaaa gccgatgtcg
    19621 tcaattactc tccggggccg gttatcactc gctttgaact taacctggct cctggcgtaa
    19681 aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg acggtggcgg
    19741 tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta ccaaataaaa
    19801 aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc gataatccgt
    19861 cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt gccgatctgg
    19921 cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct gtcggtgtga
    19981 acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt ttcatcatga
    20041 tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcatctg ttaacagaag
    20101 tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac gagatggagc
    20161 gtcggtataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa
    20221 ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag ccgggtgaca
    20281 gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtggtg ttggttgacg
    20341 aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata gcacgtctgg
    20401 cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt ccatcggttg
    20461 atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt accgtatcca
    20521 gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg ctggggatgg
    20581 gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat ggtgcttttg
    20641 ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt cgcccacagt
    20701 atgttgatgg catcacttcc gacagtgaaa gcgaaggtgg cgcgggcggt ttcgatggcg
    20761 ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag
    20821 cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca gcgcgtatta
    20881 tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt aatcgtgaag
    20941 tgctggcccc accgccgttt gactaattaa tgcattgccg gataaggcgc ggtagcgtcg
    21001 catccggcac tctatcaact gaaaattcag tattttcttc tttcctcaag ctgattatta
    21061 gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg aggaataatg
    21121 atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg
    21181 gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc
    21241 ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggtca gggcgatctg
    21301 tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga aagcattctg
    21361 gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc tacggcaacc
    21421 tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa ccagtccagc
    21481 gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc
    21541 agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac aatccatcag
    21601 tttagcgcgg tggagcagga cgatcagcgc agcagttatc aactgaaatc ccagcaaaat
    21661 ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac ggtagatgat
    21721 caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg ataatacttt
    21781 tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg gccagcaaca
    21841 tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatt tacattctat
    21901 gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta
    21961 tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag agattcgcga
    22021 ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga
    22081 cgaagttcac cgtttcaaca aaagccagca ggatgcattt ctgccacata ttgaagacgg
    22141 caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta attcggcact
    22201 gctttcccgt gcccgtgtct atctgttgaa atccctgagt acagaggata ttgagcaagt
    22261 actaactcag gcgatggaag acaaaacgcg tggctatggt ggtcaggata ttgttctgcc
    22321 agatgaaaca cgacgcgcca ttgctgaact ggtgaatggc gacgcgcgcc gggcgttaaa
    22381 tacgctggaa atgatggcgg atatggccga agtcaatgat agcggtaagc gggtcctgaa
    22441 gcctgaatta ttgaccgaaa tcgccggtga gcgaagcgcc cgctttgata acaaaggcga
    22501 tcgcttttac gatctgattt ccgcactgca taagtcggta cgtggtagcg cacccgatgc
    22561 ggcgctgtac tggtatgcgc gaattattac cgccggtggc gatccgttat atgtcgcgcg
    22621 tcgctgtctg gcgatagcgt ctgaagatgt gggtaatgct gatccacgag cgatgcaggt
    22681 ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg aacgcgccat
    22741 tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt
    22801 taaagccgcg ctggcggatg ctcgcgaacg tccggattat gacgtgccgg ttcatttgcg
    22861 taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc gttacgctca
    22921 tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa tagcacaaac
    22981 acgctattat ttcccgacaa acaggggcct tgaaggcaag attggcgaaa agctcgcctg
    23041 gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt atcgttgcgg
    23101 taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca tttaattcga
    23161 taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc
    23221 tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg ctcttgaaga
    23281 gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta actcccgatc
    23341 gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac gtctggaagt
    23401 gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt tacaggctga
    23461 aattcgcgat atcgcgctaa ccatccctaa cttgcctgca gatgaagtgc cggtaggtaa
    23521 agacgaaaat gacaacgttg aagtcagccg ctggggcacc ccgcgtgagt ttgactttga
    23581 agttcgtgac cacgtgacgc tgggtgaaat gcactctggc ctcgactttg cagccgcagt
    23641 taagctgaca ggttcgcgct ttgtggtgat gaaagggcag attgctcgca tgcaccgcgc
    23701 actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg agaactatgt
    23761 tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga aatttgctgg
    23821 cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact atgcgctgat
    23881 cccaacggca gaagttccgc tgaccaacct ggtacgcggt gaaatcattg atgaagatga
    23941 tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg gttcatatgg
    24001 tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa tggtgcagat
    24061 cgtgcgcccg gaagactcaa tggcggcgct ggaagagatg actggtcatg cagaaaaagt
    24121 cctgcaactg ctgggcctac cgtaccgtaa aatcatcctt tgtaccggcg acatgggctt
    24181 tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca cctaccgcga
    24241 gatctcttcc tgctccaacg tttgggattt ccaggcgcgt cgtatgcagg ctcgttgccg
    24301 cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg gtctggctgt
    24361 tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc gtattgaagt
    24421 accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat acccaatttt
    24481 tctgaatcta aaaagcgcct gcgggcgctt tttttgtctc cctttgatac cgaacaataa
    24541 ttactcctca cttacacgta atactacttt cgagtgaaaa tctacctatc tctttgattt
    24601 tcaaattatt cgatgtatac aagcctatat agcgagctgc tatagaaata attacacaat
    24661 acggtttgtt actggaatca atcgtgagca agcttgagtg agccattatg aaaacgaaaa
    24721 tccctgatgc ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa acgacagcga
    24781 tcggcggcct ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg
    24841 ctgtcgatag cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag
    24901 ttaactgtgg tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg
    24961 tcgaaacgga caataccggc gatgacaatt acgacggcct gcaccaggtt cgcgcctgtc
    25021 tgcgtggacg ttccatgcgt cgccgtgtct ataatccgga ccgcctgaaa tatccgatga
    25081 aacgagtcgg ggcgcgcggt gaaggcaaat ttgagcgcat tagctgggaa gaagcctacg
    25141 acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgaa tccatctatc
    25201 tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccc ccgggaaata
    25261 ccctggtcgc gcgcctgatg aactgctgcg gcggttatct gaaccattac ggtgactact
    25321 cttccgcgca aattgcggaa ggtttgaact atacctacgg cggctgggca gatggtaaca
    25381 gcccgtcgga tatcgaaaac agtaaactgg tagtgctgtt tggtaataac cctggcgaaa
    25441 cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag aaatctaatg
    25501 cccgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt
    25561 ggatccctat tcgtccggga acagatgccg cactggttaa cggtctggcg tacgtcatga
    25621 tcactgaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc tacgatgaga
    25681 aaaccctgcc agccagtgcg ccgaaaaatg gccactataa agcttatatt ctgggtgaag
    25741 ggccagatgg cgtggctaaa acaccggaat gggcctcaca aatcaccggt gttccggcag
    25801 acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt atcagccagg
    25861 gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg
    25921 cgattctgac cggtaacgtt ggtattaacg gaggcaacag cggcgcgcgt gaaggttcat
    25981 acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc agcatttcga
    26041 tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg cgtgatggtg
    26101 tacgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat gccggtaact
    26161 gcctgattaa ccagcattct gaaatcaacc gtacccatga aatccttcag gatgataaga
    26221 agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca
    26281 tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcgctggat gcatcctgcg
    26341 ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga
    26401 ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg
    26461 aaggccgtac acaggaagag tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc
    26521 ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag cgcgacccac
    26581 aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac ccactgacta
    26641 cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg
    26701 aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc tttgaaagtt
    26761 atcaggatcc gctgaacaaa cagtatccgc tgcagcttac gggtttccac tataaatctc
    26821 gcgttcactc aacttacggc aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt
    26881 ggatcaaccc gcttgatgcg caaaaacgcg gtatcaacaa cggcgataaa gtcaggatct
    26941 ttaacgatcg tggtgaagtt catattgagg cgaaagtgac gccacgaatg atgccgggtg
    27001 tggtcgcact gggtgaaggt gcctggtatg acccggatgc aaaacgtgtc gataagggcg
    27061 gttgtattaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac
    27121 atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga
    27181 ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc ctgtaaagac
    27241 tacaaagatt tgacgccaga agtcagcttc cgccgcattt atgaatatgc tggcggcgac
    27301 tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct gtcgatttca
    27361 tgtaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa
    27421 cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg ctactgtcat
    27481 atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat gaccaaatgc
    27541 gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga atcttgcccg
    27601 ctgcgggcgc tggatttcgg tcctatcgac gagctgcgta aaaaacatgg cgatctggcg
    27661 gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat caaacccaat
    27721 gccaacagcc gcccgaccgg ggataccact ggctacctgg caaacccgaa ggaggtgtga
    27781 gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg ggcaatgtgt
    27841 agcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc gagcagaagc
    27901 ccagcagcgt gttatcgcct gcatgtttgg cttatgggtg ctgatgggca ttggctttat
    27961 cgcctctatg ctccatcttg gttcaccgat gcgcgccttt aactcgctca accgggtagg
    28021 ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgctg taggcggcat
    28081 cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc tatggctggt
    28141 ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat
    28201 tgataccgtt ccgacctggt acagcatctg gacgccgatg ggcttcttcc tgacgatgtt
    28261 catgggcggc ccgttactgg gttacctgtt gctgagtctg gctggcgtcg atggctgggc
    28321 gatgcgtctg ctgccagcca tttctgtact ggcactggtg gtgagtggtg tgatgtcagt
    28381 gatgcagggc acagagctgg caactattca tagttctgtg cagcaggccg cagcgctggt
    28441 gccggactat ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg ccctgtgctt
    28501 gtggattgca ccacagctaa aaggttatca gcctgcggtt ccgctactat ccgtctcatt
    28561 cattctgctt ctggcagggg aattaatcgg tcgcggcgta ttctatggtt tgcatatgac
    28621 cgtggggatg gccgtcgcaa gctaatcagt aacaaccggg gtttcggccc cggttctctt
    28681 ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa ttacgataat caggaatatg
    28741 gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcacggt gcagctcgca
    28801 cgccacgccg aaccatgtca tcaattgcgc acctgcttgc gacatgcgat cccatgccga
    28861 atgccgggta atttcattaa aggtccctga cgcgtcagtc acgacaaaga cgtcaaaccc
    28921 ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat
    28981 aattaactgt tttttacctg tcgctttgac tgcttttaca aaatcttcgt tatcccaggc
    29041 gttaatattt cccgggcgag caatgtaagg tgcatcgggg aattgtgctt tcagttctgg
    29101 aactaatgga ccgttaggac cggtttcaaa actggtggta agaatagttg gtaagttgaa
    29161 atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg gttcgatatc
    29221 ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacggcag catcattttt
    29281 atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgtct
    29341 gaaatcggtc agacgcgatg cattgctctg aaagcataga cgggaaatat gagtttgctg
    29401 tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac
    29461 aatcagatga gattaatcgt ctcttcagca ttagcgttat gaatgagtaa tataaatcga
    29521 taataccgcc gctgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc
    29581 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc
    29641 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata
    29701 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
    29761 gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaagc
    29821 gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg
    29881 gccttggcct gatgattgga ttctggagct ggttggcttg gcgttttgtc gcgggcgtcg
    29941 gctgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta
    30001 accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg tttttaggcc
    30061 agttactggt cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg gttacaggtt
    30121 tgacgttggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag caggctgaaa
    30181 accatgattc gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg cttggcgtga
    30241 atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg ccgctgtacc
    30301 tcaatcacaa aggggtgagc aatgccagca ttggtttctg gatggcggta ctggtcagtg
    30361 cgggtatcct tggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg
    30421 tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt agccaggcgg
    30481 cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga
    30541 tggcatgggc ttgcgagaaa gttgaacatc atcaactggt ggcgatgaac caggccttac
    30601 tgttgagtta tactgtggga agtctgcttg gcccgtcatt taccgctatg ctaatgcaga
    30661 atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga
    30721 tgctgctgcg caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa
    30781 gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt
    30841 tattccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt ttgcatttcg
    30901 ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg cccctttaat
    30961 tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagttgc gatggtacaa
    31021 cattgcgctg atgtctttta tcactgtctg gggttttggc aacgttgtta ataactatgc
    31081 caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcactct atttcacacc
    31141 ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg gcggggtcag
    31201 tacctggatt aaacacacga tgggacccgg actggcttat ctcgccgcgt ggacctactg
    31261 ggtggtgcat attccctatc tggcacaaaa accccaggca attctgattg cgctcggttg
    31321 ggcgatgaaa ggcgacggtt cgctaatcaa agaatattca gtcgtagcgt tacaggggtt
    31381 aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga aatcgctgaa
    31441 aatcgtcggt tctgtggcag ggattgcgat gtttgttatg tcgctcctgt atgtggcgat
    31501 ggcggtaacc gcgcctgcga ttactgaagt gcatattgcg accacaaaca ttacctggga
    31561 aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc tggttttcgc
    31621 ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc caggaaaaga
    31681 atttccaaaa gggatgctat gcctggcggt gatggttgcg gtttgtgcca ttctgggctc
    31741 actggcgatg gggatgatgt ttgactcgcg taatatcccg gatgacttaa tgactaacgg
    31801 tcaatattac gcctttcaga agctgggcga gtattacaac atgggtaata ctttaatggt
    31861 gatttacgcc attgcgaata ccctgggaca agtagctgcg ctggtattct cgattgatgc
    31921 cccgcttaaa gtgctattag gtgatgctga cagcaaatat attccagcca gtttatgtcg
    31981 taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtgc tggtggcaat
    32041 tctgattatg ctgccgactc tcggcatcgg tgatatgaac aatctctata aatggctgtt
    32101 gaatcttaac tcggtagtga tgccgctgcg ttatttatgg gtatttgttg catttattgc
    32161 agtcgttcgc ctggcgcaga aatataaacc agaatatgtc tttattcgta ataagcctct
    32221 ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc tttgcctgct tgacggggat
    32281 cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc tgaatgttgc
    32341 aacgccgttt gtgctggtag gattaggact gatattcccg ctgctggcgc gtaaagcgaa
    32401 tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat
    32461 aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttggataa tagatctgga
    32521 agcctgaacg ctctgtgctc cagtcttcca gtacacattc taaacgacca gtatcaaggt
    32581 actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa acggcctcca
    32641 gttcagcatc aacatcatcc agaatgatat ttcctgcgac agcaatttcc agctcctttg
    32701 caaattgcca gtggaaaggt ttgccgctgg gataacgaaa aacaacacat tgatgattaa
    32761 gcagatcatg aggatggcgg ggtttgccat atcttgcaaa gtactctggc gttgctgcta
    32821 cacataattt cactggtggg ccgatcgcca cgctgatcat atctttttcg acgatgccac
    32881 taagtcgaac gccagcgtca aagccctgtt ggacgatatc gacaagagaa tcgtcagttg
    32941 tgagctcgac tttaatatca ggatattcac gtgtgaaacc aaccagtaaa ggcattaaaa
    33001 atatacgtgc agctacccgg gcggcattga ttttgagcgt tcctgtcggt gtcagacgaa
    33061 aatcattcat ctcatctaac atgatctgaa tttcgtcgaa agctgggcgc aatcgttcat
    33121 agagattaga acccgcttcc gttaacgata cgcttcgggt tgtccgattg aataagcgaa
    33181 ttttaagacg ttgttccagt gttttaatac tatggctaat ggcggacgag gataagccta
    33241 attcatcgcc cgctgcacga aagctttgat tacgggccac ggcaaagaaa gtggcaaagt
    33301 cagacatatt catccgcatt agtgaaaatc cttcattaac tcatcctgaa tcatcacgtt
    33361 tacaggatta tgccgcctgc gtaaagtgcg ctccagaact taacgtggag gtaaaattat
    33421 gcagtctgaa cgtatttatt tggtatgggc ccatcctcgt catgattcat tgaccgcaca
    33481 tattgctgat gcgatccatc agcgggcaat ggagcggaaa atacaggtga cggaactcga
    33541 tttatatcgg cgtaatttca acccggtgat gacgtcggaa gatgaaccag actggaagaa
    33601 tatggataaa cgttattctc cagaggttca tcagctttat tcagagctgc ttgaacatga
    33661 cacgttagtg gtggtttttc ctctctggtg gtacagcttc ccagcaatgc taaaaggata
    33721 tattgacaga gtatggaata atgggctggc ttatggagat gggcacaaat taccattcaa
    33781 taaagttcgt tgggtggcgc tggttggagg agacaaagaa tcatttgtcc agatgggctg
    33841 ggaaaaaaat ataagcgatt atttgaaaaa tatgtgcagt tatcttggta ttgaagatgc
    33901 cgatgtcact ttcttgtgta atacagtggt attcgatggg caagaacttc atgcgagcta
    33961 ttatcagtcg ttattatctc aggtacggga tatggtagat gcactataaa atgtgttaaa
    34021 aacgctgtag cagaatgaag cgcggaataa aaaagcggca actcaataaa gttgccgctt
    34081 tacggggaaa ttagaacatt accttatgac cgtactgctc aagaatgcct ttcacgcgtt
    34141 ccatggtctc tttcttaggt ggtttaacac cgtcgagttt gtactcttca cccattgcca
    34201 cccatttgtg tttgcccagc tcgtggtagg ggaggagttc gattttctca acgttgccca
    34261 tatcacgggt aaattcaccg aggcgatgcg ctgaatcgtc atcgtcagac cagcctggga
    34321 caacaacgta gcggatccac accttcacat ttttgttcgc cagatatttg gcgaactcca
    34381 gcgtgcggtg gttggaaact ccaaccagat tttggtggat ctcgtcgttc atctgtttga
    34441 gatcgagcat taccaggtcg gttacttcca gcagttcatc aatcaccgga tcgtaacgac
    34501 gaacaaaacc gttggtgtcc agacaggtat gaatgccttc ttttttgcag gcgcggaacc
    34561 agtcacgaac aaactcagct tgtaggattg cctcaccgcc ggatgcggta acgccgccgc
    34621 cggaagcgtt cataaagtgg cgataggtca ccacctcttt cattaaatct tcaacggtaa
    34681 cttctttacc gccatgcgta tcccaggtgt cgcggttatg acaatacagg cagcgcatca
    34741 ggcagccctg gaaaaaggtg ataaagcgaa tacctgggcc gtctacggtt ccacaggatt
    34801 caaaggagtg aatgcgacca ataactgaca ttgcggtgtt tctccagatg tggcccatct
    34861 gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc tgttttggca gtcaccttaa
    34921 agtatagata gctgacaaaa aaggctctcg tgctaaaaaa ggccccactt tcgtggagcc
    34981 tttattgtac gctttttact gtacgatttc agtcaaatct aattacatag attgagtgaa
    35041 ggtacgagta ataacgtcct gctgctgttc tttagtcagc gagttgaaac gtactgcgta
    35101 gccagataca cggatggtca gctgcggata tttttccggg ttttccatcg cgtcgagcag
    35161 catttcacgg ttcatcacgt taacgttcag gtgctgacca ccttcgatgg atgcttcgtg
    35221 gtggaagtaa ccatccatca gaccagccag gttggtctta cgaacttcgt cgtctttacc
    35281 cagtgcgttc ggaacgatag agaaggtgta ggagatacca tctttagcgt aagcaaacgg
    35341 cagtttagca acggaagtca gagacgctac agcacctttc tggtcacgac cgtgcatcgg
    35401 gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg tctggggtgt taccagtttt
    35461 cttaccatac acaacgttag aagtgatggt cagaacagac tgagtcggga tagcgtcacg
    35521 gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct accaggtcaa cagccaggtc
    35581 atctacacgc ggatcattgt taccaaactg cgggtattcg ccttcgattt cgaagtcgat
    35641 agccagaccg tcttcgtcac gaatcggttt aactttcgca tatttgattg cagacaggga
    35701 gtcagcagca acggacaggc cagcgatacc acacgccatg gtgcggataa cgtcacggtc
    35761 gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg tgcatgtagt ggatgatgtt
    35821 cagtgcagtg atgtactgtt tagccagcca gtccatgaag tgatccatgc gctccatcac
    35881 ttcatcatag ttcaggacat cgcctttgat cggttcagac ttcggaccaa cctgcatttt
    35941 cagtttttcg tcaacgccgc cgttgattgc gtacagcatg gttttcgcca ggtttgcacg
    36001 cgcaccgaag aactgcattt gtttaccaac gatcatcggg cttacgcagc aagcaatagc
    36061 gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg ttctcatact gcagagaaga
    36121 ggtgtcgatg gacactttag cggcgaattt cttgaagttc agcggcagtt tttcagacca
    36181 cagaatggtc atgttcggtt ccggagacgg acccatggtg tacagggtgt tcaggaaacg
    36241 gaagctgttt ttggtaacca gggtacgacc gtcgaggccc ataccaccga tagattcggt
    36301 tgcccagatc gggtcgccag agaacagttc atcgtattcc ggagtacgca ggaagcgaac
    36361 catacgcagt ttcatgacca ggtggtcaac catttcctgc gcttcttgtt cggtgatctt
    36421 gccagctttc aggtcacgtt cgatgtacac atccaggaag gtggaggtac gaccgaagga
    36481 cattgcagca ccgttctgag acttaacagc agccaggtag ccgaagtaag tccactggat
    36541 agcttcctga gcgttggtag ccggaccaga gatgtcgtag ccgtatttcg cagccatttc
    36601 tttcatctga cccagagcgc ggtgctgttc agcgatttct tcgcgcagac ggatagtctg
    36661 ttccaggttt acgccgtttt ccagatcagc ctgcagagaa gtgaactgtg ccagtttgtc
    36721 tttcatcagg tagtcgatac cgtacagcgc aacgcgacgg tagtcaccga tgatacggcc
    36781 acggccatat gcatctggca gaccggtcag aacaccagat ttacggcaac gcaggatgtc
    36841 cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta cggtattcag tgaagatttt
    36901 tttgatcatc ggatccagtt cgcggttgta cgctttgcag gaaccttcga tcattttgat
    36961 accaccgaac gggataagag cacgtttcag cggagcttca gtctgcagac caacgatttt
    37021 ctcaagctgc ttgttgatgt agccagcgtc gtgagaggtg atggtggaag caacagcggt
    37081 gtcaaagtca actggcgcgt gagtgcggtt ttccagttta acgccttcca ttactttgtc
    37141 ccacagggtg gtggtcgctt cagtagcgcc agccaggaag gactcgtcac cctcgtacgg
    37201 agtgtagttt ttctgaatga agtcacggac gtttacttca ttctgccagt cacctttggt
    37261 aaaaccttcc caggctgtgg ctaacttttc attaagctcg gacatgtaac acctaccttc
    37321 ttaagtggat tttttattta ctgcgtactt cgacaaccat taatggtggt cgttttcacg
    37381 caggtaaatg acccagtatg tcaacccaac caacaaacca ccaccgataa tgttgccgat
    37441 cgtaaccgga atcaggttat cagtgatgaa attcatcacg gtcaggtgag aaaaattttc
    37501 cggtgcagaa ccgactgcgg tccaaaattc cggggatgcg aagtcgcgga ttacaatacc
    37561 catcgggatc ataaacatgt ttgcgatact gtgctcaaaa ccgctggcaa caaacatcgc
    37621 gaccggcagc accataatga acgctttgtc catcaggctg cggccagaat aactcatcca
    37681 tactgccaga cataccatca ggtttgccag gataccaaga cagacggcct caataaaagt
    37741 atggtgcact ttgtggtcgg cggtttgtag gacgtttagt ccccattgac catttgcggt
    37801 catatactcg ccggaaagcc acattaaaag tacaaacagc agtgcgccga ccaggttgcc
    37861 aaaatagaca tttagccagt ttttcgccaa ctgaccccag gtgatgcgcc cactcgcctt
    37921 agcaacaaca atcaacacgg tggaagtaaa gagatcggct ccgcagacaa cacaaagaat
    37981 cagccccaga gagaagcaaa tgccgccaac cagttttgcc atgccgaagg gcattgtgcc
    38041 tgtgccagtg gttgctgtga tatagaagac gaatgcgatt gagatgaaaa caccggcggt
    38101 aatcgccaga tagaaagtct taagcggatg tttcgttgct ttatagacac ccgcctcttc
    38161 ggccactttg gccattgcag caggaagtaa aagatcaaaa gggttgtcag ctttcacact
    38221 aactctctct ttattaagtc ggcgacgaga tactaacaaa gcattataga tgagaaattg
    38281 atatagatca tatctcgcct ggcttatagg cccgtaactc gcatggtttt tatgcaaata
    38341 cggagtaaat atttgattat ccaaataaaa ataaatttta aaaattaaca aatgagttga
    38401 attttttccg catcctccgc taaaac
//
LOCUS       DAZFWP020000028        37925 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFWP020000028 DAZFWP020000000
VERSION     DAZFWP020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.028"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(96..383)
                     /gene="yebO"
                     /locus_tag="ACHK0Z_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     CDS             complement(96..383)
                     /gene="yebO"
                     /locus_tag="ACHK0Z_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU4501350.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(458..601)
                     /gene="mgrB"
                     /locus_tag="ACHK0Z_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             complement(458..601)
                     /gene="mgrB"
                     /locus_tag="ACHK0Z_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU4501351.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            760..999
                     /gene="yobH"
                     /locus_tag="ACHK0Z_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             760..999
                     /gene="yobH"
                     /locus_tag="ACHK0Z_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU4501352.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1142..1933)
                     /gene="kdgR"
                     /locus_tag="ACHK0Z_RS06510"
                     /old_locus_tag="pgaptmp_001302"
     CDS             complement(1142..1933)
                     /gene="kdgR"
                     /locus_tag="ACHK0Z_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU4501353.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2110..3483
                     /gene="yebQ"
                     /locus_tag="ACHK0Z_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             2110..3483
                     /gene="yebQ"
                     /locus_tag="ACHK0Z_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4501354.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3529..4410)
                     /gene="htpX"
                     /locus_tag="ACHK0Z_RS06520"
                     /old_locus_tag="pgaptmp_001304"
     CDS             complement(3529..4410)
                     /gene="htpX"
                     /locus_tag="ACHK0Z_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU4501355.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4602..6650)
                     /gene="prc"
                     /locus_tag="ACHK0Z_RS06525"
                     /old_locus_tag="pgaptmp_001305"
     CDS             complement(4602..6650)
                     /gene="prc"
                     /locus_tag="ACHK0Z_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU4501356.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6670..7368)
                     /gene="proQ"
                     /locus_tag="ACHK0Z_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             complement(6670..7368)
                     /gene="proQ"
                     /locus_tag="ACHK0Z_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU4501357.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7465..7962)
                     /gene="msrC"
                     /locus_tag="ACHK0Z_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             complement(7465..7962)
                     /gene="msrC"
                     /locus_tag="ACHK0Z_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU4501358.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8092..9375
                     /gene="yebS"
                     /locus_tag="ACHK0Z_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             8092..9375
                     /gene="yebS"
                     /locus_tag="ACHK0Z_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU4501359.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9344..11977
                     /gene="yebT"
                     /locus_tag="ACHK0Z_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             9344..11977
                     /gene="yebT"
                     /locus_tag="ACHK0Z_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU4501360.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12057..13496
                     /gene="rsmF"
                     /locus_tag="ACHK0Z_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             12057..13496
                     /gene="rsmF"
                     /locus_tag="ACHK0Z_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU4501361.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13614..13850
                     /gene="yebV"
                     /locus_tag="ACHK0Z_RS06555"
                     /old_locus_tag="pgaptmp_001311"
     CDS             13614..13850
                     /gene="yebV"
                     /locus_tag="ACHK0Z_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU4501362.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13955..14146
                     /gene="yebW"
                     /locus_tag="ACHK0Z_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             13955..14146
                     /gene="yebW"
                     /locus_tag="ACHK0Z_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU4501363.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14147..14803)
                     /gene="pphA"
                     /locus_tag="ACHK0Z_RS06565"
                     /old_locus_tag="pgaptmp_001313"
     CDS             complement(14147..14803)
                     /gene="pphA"
                     /locus_tag="ACHK0Z_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4501364.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15199..15540)
                     /gene="yebY"
                     /locus_tag="ACHK0Z_RS06570"
                     /old_locus_tag="pgaptmp_001314"
     CDS             complement(15199..15540)
                     /gene="yebY"
                     /locus_tag="ACHK0Z_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU4501365.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15553..16425)
                     /gene="copD"
                     /locus_tag="ACHK0Z_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             complement(15553..16425)
                     /gene="copD"
                     /locus_tag="ACHK0Z_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU4501366.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16429..16803)
                     /gene="yobA"
                     /locus_tag="ACHK0Z_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             complement(16429..16803)
                     /gene="yobA"
                     /locus_tag="ACHK0Z_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU4501367.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16942..17172
                     /gene="holE"
                     /locus_tag="ACHK0Z_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             16942..17172
                     /gene="holE"
                     /locus_tag="ACHK0Z_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU4501368.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17274..17930
                     /gene="yobB"
                     /locus_tag="ACHK0Z_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             17274..17930
                     /gene="yobB"
                     /locus_tag="ACHK0Z_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU4501369.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17954..18616
                     /gene="exoX"
                     /locus_tag="ACHK0Z_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             17954..18616
                     /gene="exoX"
                     /locus_tag="ACHK0Z_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU4501370.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18613..20673)
                     /gene="ptrB"
                     /locus_tag="ACHK0Z_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             complement(18613..20673)
                     /gene="ptrB"
                     /locus_tag="ACHK0Z_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU4501371.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20882..21541)
                     /gene="yebE"
                     /locus_tag="ACHK0Z_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             complement(20882..21541)
                     /gene="yebE"
                     /locus_tag="ACHK0Z_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4501372.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21868..22224)
                     /gene="yebF"
                     /locus_tag="ACHK0Z_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             complement(21868..22224)
                     /gene="yebF"
                     /locus_tag="ACHK0Z_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU4501373.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKVGMAEYQRR"
     gene            complement(22291..22581)
                     /gene="yebG"
                     /locus_tag="ACHK0Z_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             complement(22291..22581)
                     /gene="yebG"
                     /locus_tag="ACHK0Z_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU4501374.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22715..23893
                     /gene="purT"
                     /locus_tag="ACHK0Z_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             22715..23893
                     /gene="purT"
                     /locus_tag="ACHK0Z_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU4501375.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23949..24590)
                     /gene="kdgA"
                     /locus_tag="ACHK0Z_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /gene_synonym="eda"
     CDS             complement(23949..24590)
                     /gene="kdgA"
                     /locus_tag="ACHK0Z_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4501376.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24627..26438)
                     /gene="edd"
                     /locus_tag="ACHK0Z_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             complement(24627..26438)
                     /gene="edd"
                     /locus_tag="ACHK0Z_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU4501377.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26673..28148)
                     /gene="zwf"
                     /locus_tag="ACHK0Z_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             complement(26673..28148)
                     /gene="zwf"
                     /locus_tag="ACHK0Z_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU4501378.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     YNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28486..29355
                     /gene="hexR"
                     /locus_tag="ACHK0Z_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /gene_synonym="yebK"
     CDS             28486..29355
                     /gene="hexR"
                     /locus_tag="ACHK0Z_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU4501379.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29483..30925
                     /gene="pyk"
                     /locus_tag="ACHK0Z_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             29483..30925
                     /gene="pyk"
                     /locus_tag="ACHK0Z_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU4501380.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31056..32027)
                     /gene="lpxM"
                     /locus_tag="ACHK0Z_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /gene_synonym="msbB"
     CDS             complement(31056..32027)
                     /gene="lpxM"
                     /locus_tag="ACHK0Z_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4501381.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32147..33469)
                     /gene="mepM"
                     /locus_tag="ACHK0Z_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             complement(32147..33469)
                     /gene="mepM"
                     /locus_tag="ACHK0Z_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU4501382.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33485..34417)
                     /gene="znuA"
                     /locus_tag="ACHK0Z_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     CDS             complement(33485..34417)
                     /gene="znuA"
                     /locus_tag="ACHK0Z_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU4501383.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34496..35251
                     /gene="znuC"
                     /locus_tag="ACHK0Z_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             34496..35251
                     /gene="znuC"
                     /locus_tag="ACHK0Z_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU4501384.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35248..36033
                     /gene="znuB"
                     /locus_tag="ACHK0Z_RS06670"
                     /old_locus_tag="pgaptmp_001334"
     CDS             35248..36033
                     /gene="znuB"
                     /locus_tag="ACHK0Z_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU4501385.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36180..37190)
                     /gene="ruvB"
                     /locus_tag="ACHK0Z_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             complement(36180..37190)
                     /gene="ruvB"
                     /locus_tag="ACHK0Z_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU4501386.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37199..37810)
                     /gene="ruvA"
                     /locus_tag="ACHK0Z_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             complement(37199..37810)
                     /gene="ruvA"
                     /locus_tag="ACHK0Z_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU4501387.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9088 a         9798 c         9348 g         9691 t
ORIGIN      
        1 gttttaattt gaagcagttg caccatggca gtgcgtataa tcaggatgaa ttgagtaacc
       61 cttcaacgac ggggttactc aagattgagg aaggattatc gttcggcgac cagacgaata
      121 atatcttcgt cttcaaccga tttttgactc tcaacggtct ttttatctgc tttctcaggt
      181 tcgtttgctt cgcacaaacg acgtaacagt gcattttgac gtttttgctg atccagcaac
      241 gcctcaagca gttcaatctg ttcgttagtc cgagaactgg cacgattaat aaaaaaccac
      301 aagatgagcc cgataagaag aaccaccacc gatacaacca aagacgcaat gttcatcacg
      361 cctgaattta caacttcgtt catttcacca cctctgagta gaggcgctat tctaccactg
      421 ctggagagga agaaaatcta gtgctgaaaa aatgatatca ccacgggata aactggttaa
      481 tggcacaaat tccgctgaaa aattgtacat cctgatcgca catcatgttg aatacctgcg
      541 cccaaagcag caagcaagcc aacaccacga caaccagaac gacccatcga aactttttca
      601 ctccaatctc cgtttcacct accttatgtc attaaactag catgcgttag ttaaacagcg
      661 cctaactatg tcgatattcg tgctttttcg gaatgagtcg cttgcttcag cgcatattgc
      721 cgctacgatt aagcgaacat aaaaagagaa gaggttgtaa tgcgattcat cattcgcaca
      781 gttatgctga tagcactggt atggatagga ttattactca gcggatatgg cgttttgatt
      841 ggaagcaaag agaatgccgc aggattaggt ttgcaatgta cctatctgac tgccagaggc
      901 accagcacgg tacaatactt gcacactaaa agtggtttct tggggataac agattgccca
      961 ctattacgta aaagcaatat cgtggtcgat aatggttaag ggtaataaat aaccagattc
     1021 tcactcatga gtaaaatatt cttatagcaa gcgcgtttaa tataacgcac cttcaacttt
     1081 atttttctca tgctaaataa agaacctgtc tgtttgatat tccgacaggt tcttactctc
     1141 atcagaacgg atagtcgtga taacccattt gggcagaaat tttgcgcgct gcggtatgca
     1201 acattgcgac atattcctgt aaacgctctt cagagaaacg caacgtcggg aaggagatgc
     1261 tcaaaccggc aatgaccacg ccaaagcgat caaataccgg taccgcaatg catcgcagcc
     1321 cttcttcctg ctcttcgtta tcttcgccat acccctgctc gcgcacctgg tccagaacgg
     1381 gtaataacgc ttctgtactg gtgatggtcc gctcggtact gcgtttatac tccacgccct
     1441 caagaatttg cttcacttca tcgagatcgc gccatgccag cagtacctta ccaatcgcgg
     1501 tgctgtacag cggattacga cgcccaatcc gtgaatacat gcgcaaattg tacatagagt
     1561 caattttgtg tatgtaaaca atactgtctt cgtccagtgc gccgaggtgg atagtttctt
     1621 tggtcaggcg ggagatctca cgcatctgga tatctgcgct acgaattaaa tcgacgtttt
     1681 gtaacgcgcg agcgcccagt tcaaacaatt tcagggtcag ggaatatttc tccgactccc
     1741 cttcctgcgc cacataacct aaggttttca tggtctgtaa aaagcgataa acggtgcttt
     1801 ttgacatcat gacgcgctgc gacagctcgg ttatccctat ttcgcgctct tcacccagcg
     1861 cctgcaaaat gccaaaaact tttagcacgg aagatacaga atcaggctgt ttatccagat
     1921 ctgcgttagc catttatcac ctcattgcga gtgttttata aaaatcagaa ctgtttttta
     1981 ttataatttc gcaccagggt ggtcgcaatc catcttttgc cggttagtta caattctgcg
     2041 acatccaccg tgaatatcag tgctagaatc atacccctgt tgattattca ccaaagatat
     2101 aaaattccta tgccaaaagt tcaggccgac ggcctgccat tgccccagcg atacggtgcg
     2161 atattaacca ttgtgattgg catttcgatg gctgtccttg acggcgcaat cgccaacgtc
     2221 gccctgccaa caatcgccac ggaccttcat gccacgccag ccagttccat ctgggtagtg
     2281 aacgcctatc aaatcgccat tgtcatctcc ctgctctcgt tttcgtttct gggcgatatg
     2341 tttggctatc gacgtattta taaatgcggt ctggtcgttt ttctgttgtc ttcactgttc
     2401 tgcgcccttt ctgattcgct gcaaatgctc acccttgcgc gtgtcataca aggtttcggc
     2461 ggtgcagcgt tgatgagcgt taataccgca cttatccgcc tgatctatcc acaacgtttt
     2521 ctggggagag ggatgggcat aaactcgttt attgttgccg tctcttctgc tgccgggccg
     2581 acaattgctg cagcaatcct ctccatcgca tcctggaaat ggttattttt aatcaacgta
     2641 ccgttaggta ttatcgccct gcttctggcg atgcgttttc tgccacccaa tggttctcgc
     2701 gccagtaaac cccgtttcga cctgcccagc gccgtgatga acgcgttaac cttcggcctg
     2761 cttatcactg cgttgagtgg tttcgctcag gggcaatcgc tgacgttaat tgctgcggaa
     2821 ctggtggtaa tggttgttgt tggtattttc tttattcgcc gccagctttc tcttcccgta
     2881 ccgctgctac cggtggattt actgcgtatc ccgctgtttt cactttctat ttgcacatct
     2941 gtttgctctt tctgcgcaca aatgctggca atggtttccc tgccctttta cctgcaaacc
     3001 gtgctcgggc gtagtgaagt cgaaacaggt ttacttctga caccgtggcc gttagcaacg
     3061 atggtgatgg ctccgctggc aggctatttg attgaacgcg tacatgcagg attgctgggt
     3121 gctttagggt tgttcatcat ggctgcgggg cttttttccc tggttctgct gcccgcgtca
     3181 cctgcggata tcaatattat ctggccgatg atcttatgtg gcgctggatt tggcttattc
     3241 cagtcaccca ataaccacac cattattacc tccgctccgc gcgaacgtag cggtggggcc
     3301 agtggcatgt taggaacggc tcgtctactg ggtcagagta gtggcgcggc gctggtggcg
     3361 ctgatgctaa atcagttcgg tgataatggt acgcacgttt cgctgatggc tgcggctatt
     3421 ctggcagtga ttgctgcctg tgtgagtggt ttacgtatca ctcagccacg atccagggca
     3481 taaaaaaagc gcgtcgatca ggacgcgctt tttagtattt acttcatatt acttcaggta
     3541 ttcacccgta cgcagagctt caattcgttt atccagcggc gggtgagtca tgaacaactc
     3601 actgagcgat ttcgacttac cgttaatgca gaaagccatc atgctggttg cttcttgcgg
     3661 ttcatagctg gttttcaggc gctgcagcgc ggcaatcatt ttctcgcgac caaccagttt
     3721 tgccgaaccg gcatcagcat ggaattcacg atgacgcgag aaccacatgg tgataatgct
     3781 cgcaagaata ccaaacacca gttccagaac cgttgcaacc gcaaagtaga tcagcgggtt
     3841 gccgttgctc tcttcacctt catcacgatt tccgcccata aaacccgcgg caagctgcgc
     3901 cagaatacgg gaaataaaga taacgaaggt gttcaccacg ccctgaatca gcgtcatggt
     3961 gaccatatca ccattggcga tgtggctgat ttcgtgagca attaccgcct cggcttcatc
     4021 cgggctcatg ttctgcagca aaccggtgct gacagcaacc agagaggcat cacggcgcgc
     4081 accggttgca aaagcgttga tgtccggcgc atggtagata gccacttgcg gcatagcgat
     4141 ccctgcctga cgagcctggg ttgctacagt attaaccagc caacgttccc tttcgttacg
     4201 cggttgctcg atcacttccc cgccaacaga tcgtaatgcc atccatttgg acatcagaag
     4261 cgaaacgaag gaaccaccaa aaccgaacag caaggccatg atcatcagcc cctgaacgct
     4321 gctcgactgt atccctgtca ggctcagtac cagcccgaaa acgaccatta cggccaggtt
     4381 cgttagcagg aagagcgcga ttcgcatcat aattttcttt ttacctcagt ttaacaaaac
     4441 gcaatatgcg atacccacat cgtatgggtt acgcgactat tttcaagtct ggatagtgcg
     4501 taagtcacca gaaagacaca actttacatt ttgtagcatc tgatttacgg catcttgccg
     4561 ctgttaaaaa atcaggcaca atttcttgtg cctgattgat attacttgac gggagcgggt
     4621 tgttccgcgg gtctggcttt ttcaagcttc gccagatcga gtgcgatatt caccgtctca
     4681 tccagataag gatccggctc ctggtaatct ttaggtagat catccagctt cttcaactcc
     4741 ggtttacctt cgcgtttaaa gcgttcgttc aaacgcgcca gacgcgtcgc atcatcttca
     4801 ttattctctt tctcacgcac agcgtagttc agagaaacga tattgcgctt gtccttcata
     4861 gcgttgaagc gcgcgatatc cttcatgatg ttctggaact caggatcttt cgcgatacgc
     4921 gcattatgtt ccttcagcag ctccggccca aaggccgtta aatcgcctga tttcacataa
     4981 gtcgcggcat caatgctatc ccacggcagc gcgttatctt cgaatttctc acccgtttcc
     5041 gtttcttcat tacccgtcgg catgatgatg tctggcgtta cgcctttacg ttgcgtactg
     5101 ccgccgttaa cgcgatagaa tttctggatc gtgtactgca cagaacccag cgctggccat
     5161 tcaggacgta acatctgatc gtaaatacgg ttcaatgaac ggtattgctg aacggtgcct
     5221 ttaccaaacg tcggttcacc cacaaccagc gcacgaccgt aatcctgcat tgccgcggca
     5281 aagatttctg aagccgaagc actgaaacgg tcaaccagta ccaccagcgg gcctttatag
     5341 aacacctgcc cgtcggtatc gctgtcttca cgaaccttgc cgttgttatc gcggacctga
     5401 acaatgggac ccgcaggaat aaacagacct gagagcgata ccgcttcggt cagcgcccca
     5461 ccgccattgc tacgcaggtc gatgatcaca ctgctgacat tctgtttttc cagtttctgc
     5521 agttgaactt tgacatcgtc tgtcaaaccc acatagaagc ccggaatatc cagcacgccg
     5581 actttctctt taccgacggt cttcaccgac attttaaccg cgcggtcttc gagacgaata
     5641 cgttcacggg tcaatgttac agtgcgggtc ttggtccctt taccagcagg taaaatttcc
     5701 agacgaactt tactgccctt cggcccttta attaaggcaa ccacatcatc aagacgccag
     5761 ccaatcacgt caaccatcgg cttgcctgtt tgaccaacac cgacaatttt gtcaccaacg
     5821 ctgatagctt tactcttcgc tgccggacca cctgccacca tcgaattgat aacggtgtag
     5881 tcatcatcca tttgcagcac tgcgccaata ccttccagcg acaaactcat ttcagtgttg
     5941 aactgttcgg tattacgcgg ggaaagatag ttggtatgcg ggtcgatttc acgcgcaaac
     6001 gccgtcattg ccagcgagaa aacatcttcg ctgttggttt gcgccagacg acgaatggca
     6061 aatttgtagc ggcgtgtcag ggtttcacga atttctttat ccgtttttcc tgccagcttc
     6121 aggcttaact cgtcgaattt gactttactg tcccacagcg cgttcaactc agcctcgttt
     6181 ttcggccagg gcgctttgct gcggtcaagg ttataagtgt cgttgccggt gaaatccatc
     6241 ggcttttcca gtaccgacaa agcgtactgg taacgctcaa aacggcgctt ttgcgccaga
     6301 ttgtagagat cgtagaaaac gtcgagtttg cctgaacgca gttcatcgcc taactcggtt
     6361 ttctttttcg cgaactgttc aacatcgctt gccagcagca cgttgtggct gtaatcgagc
     6421 agattcaggt agcggtcaaa gattttggcc gaaaatgcct gatcgaggtc gaactggcga
     6481 taatgagaac gggtgaagcg cgacgttacg cgctcactta ccgtcgcatg ctgcgtctct
     6541 tcctttaata ccggaatttg atcagcacgc gtgatatctt ctacagcgaa ggtctggcct
     6601 gctattgcaa gcaggccagc taacgcggta agcctaaaaa acatgttcat gcctggcccg
     6661 gcctccgttt cagaacacca ggtgttctgc gcgcacaatc aaagacatac ccgaattcag
     6721 ctggacgcgg acgccgtctt tggtgatttc taatacggtg gcatccatcg cgttttgacc
     6781 cgctttcacc ttcagggctt gtccgacagt cagagctgaa atgtcagaaa ccggggtgtg
     6841 ctgttcttcg cgaggtgctt ttactgtttt tggcgctttc tctaccggct tttgcgcacg
     6901 aggtttacgt tcagcgcctt ctttgcggcg tggcgtggtc ggacgtggct tgcgttcgcg
     6961 gcgcggtgcg tcttctttct caccagcagc tgcggcagct tcgcgttttt tcgcttgctg
     7021 ttcagcacgc tgtgcctgaa cacgcgcttt cgcttcttca agctgcttgc gagcatgctc
     7081 tacatgttgc tcgtccagct caccgcatgg gttgccgtca agatcgacac gcgttgcgcc
     7141 gggtttaaca ccgtaaagat aacgccagct cgaagtgtag agacgtaaag cggatcgtaa
     7201 ttgcgttttg ctcaggttca tttccccggc aacacgatcg accaaatcct gaaaaatacc
     7261 gattttcagc ggacgcgctt caccttccgc actgaaacag tggggaaaac gttcggccag
     7321 aaacgcgatt acttctttac tgctattcaa cttaggttga ttttccatga aatttcctga
     7381 ttacaacgga cgtagccaac aagcgcaggc atgaacaggc gtcattataa tgacgctacc
     7441 agtaaatgct acgttatccg ttgattatcc tgcgacgctc gcaaagaatt ttttgtaatc
     7501 cgtcgttgca agcacttttt caagctgtgc cacaagctga cgtaagcctt gctcgtcctc
     7561 gtctgtaaag cgaccgaaga cggtactatc gatgtcaaga acaccaataa tctgattttt
     7621 caccaccagc ggcagaacaa tttcagaatt actcgccgca tcacaggcaa tatgcccgtc
     7681 aaacgcgtga acatcctcga tacgctgcac ttgattgcgg gcaaccgcag tgccgcacac
     7741 cccgcgcccg acgggtatcc ggacacaggc aattttgccc tgaaatggtc ctagtaccag
     7801 tgtatcgtcc tcaagcaaat aaaaacctgc ccagtttacg tcagtgagac gctcgtataa
     7861 caacgcactg gtgttcgcaa gcgttgccag aaaactggtt tctcccgcca tcagcgcgtt
     7921 aaagtcgcga tttaaatccg cgtaaaattc tgttttgttc attatataat cacttggttg
     7981 tcttacctgg aactgccagc ctattaaaat aagcattaaa tgcgttaatg ctcaagatca
     8041 ttccaatcat gggttaatat taatgttaat tcttattaca tttggcacgt catggctctt
     8101 aacacaccac aaattacgcc gacaaaaaag ataacagtga gggcaatcgg cgaggaactg
     8161 ccgcgtggtg attaccaacg ttgcccgcaa tgtgacatgc tgtttagcct gcccgagata
     8221 aattctcatc agagtgccta ttgtccgcgc tgtcaggcaa aaattcgcga cgggcgcgac
     8281 tggtcgctaa cgcgcctggc ggcaatggct ttcaccatgc tgttgctgat gccgtttgcc
     8341 tggggcgaac cgctgttgca tatctggctg ttaggtattc gtatcgacgc caacgtcatg
     8401 caaggcatct ggcaaatgac caaacagggc gatacgataa cgggggcgat ggtctttttc
     8461 tgcgttattg gtgcccccct cattctggtg tcctccatag catatttatg gtttggtaac
     8521 cgactgggaa tgaatttacg tccggtactg ctgatgcttg agcgacttaa agagtgggta
     8581 atgctggata tctacctggt cggcattggc gttgcttcta taaaggtaca ggattatgcc
     8641 catatccagg cgggtgtcgg cttgttctct tttgtggcgt tggtgatttt aacgacggtg
     8701 acgttgtcac atcttaatgt cgaggagctg tgggagcgat tttatccgca gcgccccgct
     8761 acgcgtaggg acgagaaact tcgtgtctgt cttgggtgcc attttaccgg ctatcctgat
     8821 cagcgtggtc gctgcccgcg ttgccatatc ccgctacgcc tgcgtcgccg tcatagtttg
     8881 caaaaatgct gggcggcgct gttagcgtca atcgttttgt tgttacctgc caacctgttg
     8941 cctatttcta tcatttatct gaacggagga cggcaggaag atacaattct ttccggaatt
     9001 atgtcgctgg caagtagcaa cattgcggtt gcgggaatcg tgtttatcgc cagtattctg
     9061 gtaccgttta ctaaagtgat cgtcatgttc actttactgt tgagcattca ttttaaatgc
     9121 cagcaaggtt tacgcacacg cattctgtta ctgcggatgg tgacctggat tggtcgctgg
     9181 tcgatgctcg acctgtttgt catatcttta accatgtcgc tgattaatcg cgatcagatc
     9241 ctcgctttta ctatgggacc ggctgcgttt tatttcggcg cagcggtaat tttgactatt
     9301 cttgctgtgg aatggctgga cagccgctta ctttgggatg cacatgagtc aggaaacgcc
     9361 cgcttcgacg actgaagcgc agattaaaaa taaacgccgt atctcacctt tctggctgct
     9421 gccgttcatc gcgctaatga ttgccggttg gctgatttgg gacagttatc aggaccgggg
     9481 taataccgtc accatcgact ttatgtcggc ggatggtatt gtcccgggcc gtacgcctgt
     9541 tcgttatcag ggcgttgaag tcggaacagt gcaggatatc agcctcagcg acgatcttcg
     9601 taagattgaa gtcaaggtca gcatcaagtc cgatatgaaa gatgcgctgc gcgaagagac
     9661 tcagttctgg ctggtgacgc caaaagcatc gttggcaggt gtctccgggc tggacgccct
     9721 cgtcggtggt aactatatcg gcatgatgcc aggtaaaggt aaagagcagg atcactttgt
     9781 cgcactcgat acccaaccga aatatcgact ggacaatggc gatctgatga tccacctgca
     9841 agcccccgat ctcggttcgc tgaacagcgg ttcattggtc tatttccgca agatccctgt
     9901 gggaaaagtc tacgactatg ccatcaatcc caacaagcaa ggcgtggtga ttgatgtcct
     9961 gatcgagcgg cgttttaccg atctggtgaa aaaaggtagc cgtttctgga acgtttccgg
    10021 cgttgatgcc aatgtcagta tcagtggcgc gaaggtgaaa ctggaaagtc tggcggcact
    10081 ggttaacggt gcgattgcct tcgattcacc tgaagagtcg aaacctgccg aggcggaaga
    10141 tacctttggt ctgtatgaag atctggccca cagccagcgt ggcgtaataa taaaactgga
    10201 actgccgagt ggggcaggat taaccgccga ctcgacgcca ttaatgtatc agggactgga
    10261 agtcggacag ctaactaaac tggatttaaa tcctggtggt aaagtcactg gggaaatgac
    10321 agttgatccc agcgtcgtta ccctgcttcg tgaaaatacc cgcatcgaat tacgtaatcc
    10381 gaaattatcc cttagcgatg ccaatctcag cgccctgctg actggcaaaa ccttcgagtt
    10441 ggtacccggc gatggcgagc cacgcaaaga gttcgttgtt gtgccaggcg aaaaagcact
    10501 gctgcatgaa cctgatgttc tgacgctgac cctgaccgcg ccggaaagtt acggtattga
    10561 tgctggtcag ccgctcattc ttcacggcgt gcaggtaggc caggttatcg atcgtaaact
    10621 caccagcaaa ggcgtcacct ttaccgtcgc catcgagcct cagcatcgag aactggtaaa
    10681 aggcgatagc aaatttgtcg tcaacagccg tgtcgacgtg aaggtggggc tggatggcgt
    10741 tgagtttctc ggtgccagcg cctcagaatg gattaacggc gggatacgta ttctgccggg
    10801 cgataaaggc gagatgaaag ccagctatcc actgtatgcc aatctggaaa aagcgctgga
    10861 gaacagcctt agcgatttac ccaccacaac cgtgagtttg agtgcagaga cgctgccgga
    10921 tgtgcaggca ggatcggtag tgctctaccg taaatttgaa gttggtgaag ttattaccgt
    10981 gcgtccgcga gctaacgcgt ttgatatcga tctgcatatt aagccggagt atcgcaacct
    11041 tctgaccagc aatagcgtgt tctgggcaga aggcggggcg aaagttcagc tgaatggtag
    11101 tggcctgacc gtacaggcat ccccgctctc cagagcatta aaaggggcta ttagcttcga
    11161 taacctcagt ggtgccagcg ccagtcagcg taaaggcgac aaacgtattc tgtatgcttc
    11221 cgaaacagcg gcccgtgcgg ttggtgggca gattacgctt cacgctttcg atgccggaaa
    11281 actggcggtc gggatgccaa ttcgctatct cggtattgat atcgggcaaa tccagacgct
    11341 ggatctgatt accgcgcgca atgaagtaca ggcaaaggcg gtgctctatc cggaatatgt
    11401 ccagaccttt gctcgcggtg gtacgcgctt ctcagtggtc acaccgcaaa tttcggcagc
    11461 tggcgttgag catcttgata ctatcctcca gccgtatatc aacgtcgaac caggccgggg
    11521 caatcctcgc cgcgactttg aattacaaga ggccaccatt actgattcgc gttacctgga
    11581 tggcttaagc attattgttg aagcgccgga agccggttcg ttaggtattg gtacgcctgt
    11641 gctgttccgt ggtctggaag tcggtacggt tacaggaatg acgctgggga cattgtcaga
    11701 tcgcgtgatg attgcgatgc gcatcagtaa acgctatcaa cacctggtgc gtaacaattc
    11761 cgtcttctgg ttggcatcgg gttacagtct ggactttggt ctgacgggcg gcgtagtgaa
    11821 aaccggcacc tttaaccagt ttatccgtgg cggcatcgcc ttcgccacgc ctccaggtac
    11881 gccactggca ccgaaagccc aggaaggcaa acacttcctg ttgcaggaaa gtgaaccgaa
    11941 agagtggcgt gaatggggaa ctgcgcttcc caaataatgc ccactgctcc ggcgtgcctg
    12001 cgccggagcg tttatgctaa actgcgcgcc tgtttttttg ccagtggtac atgctcgtgg
    12061 cccaacacac cgtttatttc ccggacgcct ttctgacaca aatgcgcgaa gcgatgcctt
    12121 cgacgctctc atttgatgat tttcttgccg cctgtcagcg cccgttgcgc cgcagcattc
    12181 gcgttaatac gctgaaaatc tccgttgctg atttcctgca attaaccgct ccttatggct
    12241 ggacgcttac gccaattccg tggtgtgaag aaggtttctg gattgaacgc gacaatgaag
    12301 atgcattgcc attgggtagt accgccgagc atttaagcgg cctgttttat attcaggaag
    12361 ccagttcaat gttgcccgtt gccgccttgt ttgctgacga taatgcacca cagcgggtga
    12421 tggatgtcgc agctgcgcca ggctccaaaa cgacgcaaat tgccgcgcgg atgaataacg
    12481 aaggggcaat ccttgccaat gagttttccg ccagtcgggt aaaagtgtta catgccaata
    12541 tcagccgctg tggcatcagt aatgttgcgc tcacacattt tgatggccgc gtgtttggtg
    12601 cggcagtgcc agaaatgttc gatgccattt tgctggacgc tccctgctct ggcgaaggcg
    12661 tggtgcgtaa agatcccgat gcgctaaaaa actggtcacc agaaagcaat caggaaatcg
    12721 cagctacaca acgggagctt atcgacagcg cctttcatgc attacgtcct ggtggtacgc
    12781 tggtttactc gacctgtacc ttaaaccagg aagaaaacga agccgtttgc ctgtggctga
    12841 aagagactta ccccgacgca gtagagtttt taccacttgg cgatctcttc cctggtgcaa
    12901 acaaagcgct gaccgaagaa ggctttttgc atgttttccc acaaatttac gactgcgaag
    12961 gcttcttcgt tgctcgtctg cgtaaaactc aggcgatccc cgccttaccc gcccccaaat
    13021 acaaagtcgg taatttcccg ttcagcccgg tgaaagatcg cgaagccgga caaattcgtc
    13081 aggcggctgc aagtgttggc ttaaactggg atggaaacct gcgactctgg caacgcgaca
    13141 aagaactgtg gttgttcccg gtgggcattg aagccctgat cggtaaagtc cgattttctc
    13201 gcttggggat taaacttgcc gaaacgcaca acaaaggtta tcgctggcag catgaagcag
    13261 ttattgccct tgccaccccc gacaatgtga acgcttttga actgacaccg caggaagcgg
    13321 aggagtggta tcgcgggcgc gatgtttacc cgcaagccgc gccagtggcg gatgacgtgt
    13381 tggttacttt ccagcatcaa ccgattggtt tagccaaacg gattggttcg cgattgaaaa
    13441 acagctatcc gcgtgaactg gtgcgcgatg ggaaactttt taccggtaac gcctgacagc
    13501 gcacaaaaaa agcgcacttt ttgactggca cattcggctg cctcaactag gctgaaaaat
    13561 ggtgcgatcg gactggtcgt accacaaccg gcagctaaat ggagagcacg aagatgaaaa
    13621 ccagtgtgcg cataggcgct tttgaaatcg acgacggcga attacacggt gaatcgccgg
    13681 gtgatcgaac gttaaccatt ccttgtaaat ctgaccccga tttatgtatg caactcgatg
    13741 cctgggatgc tgaaaccagt atccctgccc tgctaaatgg cgaacactct gtcctttacc
    13801 gtacccgtta cgatcaacaa tctgatgcct ggattatgcg tcttgcctga tccaaaaaga
    13861 acccgtcggc atggcgggtt atttgtcctg gttattcccc cgttgtaaaa tctctcctaa
    13921 acttaacggt acggcaccac acttcgggga tgaaatgttc gcgctggtac tttttgtttg
    13981 ctacctggat ggcggttgtg aagatattgt tgtggatgtc tacaacacgg aacagcagtg
    14041 tctttattct atgagcgatc aacggatccg ccatggcggt tgttttccga ttgaggattt
    14101 tatagatggt ttctggcgac cagcacagga gtatggtgat ttttaattat tgcaattgca
    14161 caagagtcag ttcgccccca aagacagcac cggtatcaat ataatgcagg ttgccaatat
    14221 ccacgcgatg tcgcaacggt gtatgaccaa accagaaatg atcagcacct gtaattccct
    14281 gccctttttg gcgttcacct aatcgcgagc ggctccacaa gacctgatgc aaatcaacgt
    14341 ccttttgcca ttcataaaca tcatctggat aatcggcatg agcaataaca tgtttgccgg
    14401 tacgactgtg tacttcaaga ataaagggca aatgctgaca tttttccagc gccgttttcg
    14461 cttgtttctg ttgattatct gccagcgcaa taaaccagtc gccgccattc atcaaccaca
    14521 aagacatctg ctgggatgcc agcgcatcca tcgccatctg ttcatgattg cctcttaccg
    14581 cacgaaccca atgttgttcc agtaactgca ggcaacgtaa actttgcggc ccacgatcga
    14641 taacgtctcc cactgagata agtaaatctc gccacggatc aaaaccacaa tgccataatt
    14701 tgcggcgcaa ctgctcaaga caaccgtgta tatcgccaga aagccagata tgtcgccatt
    14761 gatgacccgc aattctctga taaacgggcg caggctgttt catcaatatt ttcctcccgc
    14821 gctaaagatc acataatctt aacaagaatg ttaaaaaacg ctggactcag acagtagagt
    14881 gtgtgttatg gttgactata aagtcagcga aggaaatgct tctggctttt aacagataaa
    14941 aagagaccga acacgattcc tgtattcggt ccagggaaat ggctcttggg agagagccgt
    15001 gcgctaaaag ttggcattaa tgcaggctta gttgccttgc cctttaagaa tagatgacga
    15061 cgccaggttt tccagtttgc gtgcaaaatg gtcaataaaa agcgtggtgg tcatcagctg
    15121 aaatgttaaa aaccgcccgt tctggtgaaa gaactgaggc ggttttttta ttggaaatca
    15181 aaaggctatt ttaggtaatt aacagagttt ttcagctcgt tctataaacg gtgccagact
    15241 cattttttcg ccgggattgt taggatcatc aatctgaatc accgaaatgg gttgggcttt
    15301 agtcttccca ctggcaactt ccttttgtgc gatatcgttt aaaggatact gcacgagggt
    15361 acttgggttg atgacataca aagcattacc cggtcggcaa gtcagcatca cctcttcgcg
    15421 attaaacgcc catttgtctt tacccacttc aaaacggctg acggtaatca cctgcggtgc
    15481 agccagcgcc gctgcagaac tggtgagtaa cagaaacgcc agaatacttt ttttcatcat
    15541 tttcgtaatc catcaaaaag gttcgagagt cgcaagcagg ctgatgatca ccagcactac
    15601 cgcacctatc gcccattcga gtttagtcat ccactcaaac cagggtgccg cacgatcttc
    15661 atcctgcctc atgcgcggta caagaacata acgattagcc agcgcaatga ccaccataat
    15721 catcaccagg atggctttga gcaataagag ttggccccag taagtcgtaa gcgtgggggg
    15781 aaatccggta atcaacaatg cattaagcac gccgcttgcc agtacgccga tcaccgcaaa
    15841 atgcccgcac caggaaaaac gcatcagcgc ctgaatggcc tggtgtcgcc aacggccttt
    15901 gatgagttgc atacaccaga gcaccggcag taatccccca aaccacgccg ccgcacaaat
    15961 caggtgaatc gcgtgattag tctgatggat tttcgccgtt accccttcat tcagtgtcgc
    16021 atgcccgatt cccgccagca gaataaattg cgcggtagtg agcataaaca gcaaacgtgg
    16081 catattccgc ggttgcataa gggcgacgat caacgttacg agggcgagaa caatttgcca
    16141 taaccagacg ccaccaaact gcgtttgcaa aacagcctgc caaatgtttg gcgaaaatac
    16201 atctgcccat cccgttccca tcaacccgcc ctgaacggcg agcattgcag tggcactgat
    16261 caaactccag acggcagcgt gctgttgtaa acgcagaaaa cgcttcgcca gcaagcgacg
    16321 aatcgttaag ggggccagcc aggcaccgta cattgcgaag ccaaagacca gcatcagcga
    16381 ggtgaaatgg ataaatcgca gcgcgatcca ggtaaacgcc agcatgattt atttcacgct
    16441 aaaggtgtag tgtcctttcg ttttatgccc gtccaccgaa acaacatgcc agtcaacggt
    16501 gtaggttccg ggtttcagcg aatcggccag tggaacaatc agttgctttt ggtcctgctc
    16561 atttcgcttc gccggtaatg ttttaatatt ttcgtttttt ggccccgtga tttttgcacc
    16621 actgaatcct gtttcaacac cttccgagaa gtttaaagtg attgcctgcg gtgcagctgt
    16681 cacttgcgcg tttgccgcag gatactgatg cgttaaatgc gcatgtgccc agacggaagg
    16741 tgtcaccaac gaagtggtca ggattgccag cgcgtagcga agggagcgtg cagttgaagt
    16801 catattatct attccttttt gtaataactt ttttacagag cataaccttg tctaatgtct
    16861 gagtcgagga tcatcaattc cggcttgcca tcctggctca ctcttagtaa cttttgcccg
    16921 cgaatgatga ggagattaag aatgctgaag aatctggcta aactggatca aacagaaatg
    16981 gataaagtga atgtcgattt ggcggcggcc ggggtggcat ttaaagaacg ctacaatatg
    17041 ccggtgatcg ctgaagcggt tgaacgtgaa cagcctgaac atttgcgcag ctggtttcgc
    17101 gagcggctta ttgcccaccg tttggcttcg gtcaatctgt cacgtttacc ttacgagccc
    17161 aaacttaaat aaaacttata cagagttaca ctttcttaca taacgcctgc taaattatga
    17221 gtattttcta aaccgcaccc ataatttgca gtcattttga aaaggaagtc attatgtcgt
    17281 tctggaaagt tgcagcagcg caatatgagc ctcgtaaaac ctcactcacc gaacaggtag
    17341 ctcatcatct ggagtttgtg agggccgccg ccaggcaaca atgtcagctg ttagtttttc
    17401 catcgctttc tttactgggg tgtgattatt cgcgacgtgc ccttcctgcc ccacccgatc
    17461 tctcactgtt agacccgctt tgttatgccg caacgacctg gcgaatgacc atcattgccg
    17521 gccttcccgt tgaatataac gatcgcttta ttcgtggaat tgcggtgttc gcaccgtggc
    17581 gaaaaacgcc tgggatctac catcagagtc atggtgcatg tctgggccgt cgttccagaa
    17641 ccatcacggt ggtcgatgaa caaccgcaag gtatggatat ggacccgacc tgttcactgt
    17701 ttactacagg gcaatgtctg ggagaacccg acctgctggc gtcggcccgt cgcttacaat
    17761 ttttttcaca tcagtactcg attgccgtgc tgatggcaaa tgcccgtggt aacagtgcac
    17821 tatgggatga atatggtcgt ctcatcgttc gcgccgatcg tggttcatta ttgttagtcg
    17881 gtcagcgttc atcacagggt tggcaaggcg atatcattcc attacgctag gctttttcgg
    17941 cctggagcat gccatgttgc gcattatcga tacagaaacc tgcggtttgc agggagggat
    18001 cgttgagatt gcctctgttg atgtcattga cggaaaaatc gtcaacccca tgagccacct
    18061 ggtgcgcccc gatcgtccta ttagtccaca agcgatggcg attcatcgca tcaccgaagc
    18121 catggtcgcc gataaaccgt ggattgaaga tgtgatccca cactattacg gtagtgaatg
    18181 gtatgtcgcg cataacgcca gctttgaccg ccgcgtactg cctgagatgc ccggtgagtg
    18241 gatttgcact atgaaactgg cccgtcgttt gtggcctggg atcaagtaca gcaatatggc
    18301 gttatataaa acacgcaagc tcaatgtaca gacgccgccg ggcctgcatc atcaccgcgc
    18361 gttgtatgac tgttatatca ccgccgcgtt gcttatcgat attatgaaca cctccggctg
    18421 gacggcagaa cagatggccg atatcaccgg acgtccgtcg ttgatgacga ccttcacctt
    18481 tggcaaatac cgtggcaaag cggtttccga cgttgccgaa cgcgatccgg gctatctgcg
    18541 ctggttattt aataacctgg acagcatgag cccggagctg cgtttaacac tgaaacatta
    18601 tctggaaaat acttagtccg caggcgtagc gggtaatgtt ccctgcgcca gcgcgaccag
    18661 aaaagcatat tccatcgcta cgccttcgta cgatttaaag cgaccagatt taccgccatg
    18721 gcctgagtcc atgtcggtac agagcaataa aagatggtca tcggttttca gctcgcgcaa
    18781 tttagcgacc cattttgccg gttcccaata ttgcacctga gaatcgtgca aaccggtcgt
    18841 taccagtaaa tgcggataag cctgtgcggt gacgttgtca tatgggctgt agcttttcat
    18901 gtactcgtaa tattgcggat cctgcggatt cccccactct tcaaactcac cagtggtaag
    18961 aggaattgat tcatcaagca tcgttgttac aacatcaaca aacggtacct gggcgataac
    19021 gccgtggaat aattccgggc gttgattaat tgcaacgccc atcaacatgc cccccgcact
    19081 cccgcccatc gcataacaaa gcgaaggaga gccatagccc agttttaaca atgcatcgca
    19141 ggcatcaaga taatcattaa acgtattttt cttcttcaga aattttccgt cttcgtacca
    19201 ttgttgcccc agctcaccac cgccgcgaac atggacaatg gcgtagacaa agccacgatc
    19261 taacaaactc aagcggctaa aactgaaatc ggcatcaata cttgcgccgt aagaaccata
    19321 gccatacacc agcaacgggt tgtgtccttt gcgaaaatgt ttgcgatggt agaccaacga
    19381 aaccggaact tcgacgccat cacgggcgac tatccacagg tgttcactgc ggtaattcgc
    19441 cgcatcaaaa ccgggcactt ctgtttgttt taatacacga cgctcaccgg tatccatatc
    19501 cagttcaaac aaagtatctg gtgtggtcat ggaagaataa ccataacgca atcgcgcggt
    19561 ttcaggttct ggattgtagg caatccaggt cacataggcc ggatcatcaa aggcaatacc
    19621 aatgacttcc cgggtcttgc ggttaatttg gcgcaaactg gttaacccgc gctgacgctc
    19681 ttcaaccacc agccagtcgg taaacagcgt aaacccttcc agcataatgt tatcgcgtgg
    19741 cggaattaac tcttcccact gttgctcatc acgcaggcgg gtacggtata agccaaagtt
    19801 tttgccatgg cggttggaac gcagataaaa ccgatgctgg tagtgatcaa ggctgtattc
    19861 gtgatctttg cggcgcggca gaaaaacaaa cggctcggca tcggccattt ccgcgtccag
    19921 caggcgaact tcactggtgg tggcgctggc caaatgaatg actacatagt gcttcgaggt
    19981 cgttttatgc aggctgacgt aataggtatc gtctttttct tcgtagatca gtttatcttg
    20041 cgatgctggc gtaccgatgg cgtgacgcca gacctgataa ggcagcagcg tcaccggatg
    20101 cttgcgaaca tagtagaaag tccaggagtc atttgcccag acaaagctgg gttcaacgtt
    20161 atccagcagt tccgggtacc agttaccagt ttccagatta cgaaaacgaa tgccgtactg
    20221 gcgtcgggaa agaaaatctt ctgccagcgc cataatggtg ttatcgggcg taatcgccat
    20281 tccgcccatc gaataaaact cactatgagc tgcgcgctta ttggcatcga gcaatgtttc
    20341 ccactcatcc cactcttcac tgaatgccga ttgacgctgg tagatagcat attcacagcc
    20401 tggttcataa atatgccgat agcggtagcc atttttgatg tagggcgcag aaacttctcg
    20461 ttgcggaatg cggtcgatga tttcctttaa gatgcgatcc tgcaaggctt gttgtgaggc
    20521 catcacccga tgaccgtaac tattttcttg ttgcaggtag tccaggactt ctggctgaga
    20581 acgcgtatcg tcccgcagcc agtagtaatt atcgatgcgc gtatcgccat gaagcgtcat
    20641 ggcgtgggga atgcgggcgg cttttggtag catgttattg ttctttctgg ttgaaacatc
    20701 ttataagggt ggcaaaactc accggggatg caagcgaaac aggggagtca ttgcttagat
    20761 gatgacaggt aatggcgcgg atatcgaatg ttatgcagac agagaaaatc agcctgttca
    20821 aatggctgtg cgattctgga tagcccgaaa tagtcaactt caggctatcc agagagcgga
    20881 attattccgc caaagtgcgt ttttgctgtt cgagatcgcg ttcaatgccg tcacgaacat
    20941 cctgggggat tttcagcgcg tcacccagtg cattcaggta actgcgttcc ataaaatggt
    21001 caatatcaat agccgcgcaa ctcaggaaat agatttccag cgcctcttct tcattgcgga
    21061 ccccggtagc caggcgttgt ggatccagcg gttgttcgat tgcctgctca atgagtacac
    21121 gcccctgctc ttccacgccg gcttcacgca attgctggtc gattgccgca cgttctttgg
    21181 catcaatatg accatcactt ttagcggcaa agaccagcgc aaggatcaaa cgttctgtac
    21241 gctcatccag cggcgtactt tgcgcgccaa actgcggttc gtcctgatgc gccgcgcgaa
    21301 ttttatcttt gtatttattc cacagcaccg tacccgctac cgctccgccg ccaaccagta
    21361 acgcgttggt gccatatttc gtaagaagtt tacgtgctga tttatttgcg accagcagcc
    21421 cagccagtcc gcctaatgcg cctggcacta acagtttgcc caatccctga tccgcagacg
    21481 aggaggtaga agaactgctt tgcccaagca gggattgcag ttgatttaac cagttagcca
    21541 tatttgctcc tcaataacca tttttagtgc ctgtagcata gcggatgagg atgtcagaaa
    21601 atgtatatcg agacaaaaga tgcgcaaata agcggggcgg taggccgtta ttcaaagaaa
    21661 ttcgcgccag gtaaggtatt tactgtatat caggtgataa gggtatttta ggtgaaagta
    21721 gtttgtcgtt ttgagtggca ttgtttgatg ctgattgccc gatgcgacgc tgttgcgtct
    21781 tctcaggcct acgattgtgg tgcgttctgg ggcggacaag gcactcgcgc cgcatccgcc
    21841 aacggtcaat cgaattatca gaagccatta acgccgctga tattccgcca ttcccacttt
    21901 gcagtccacg ctgacctggt aatgaatatc ggcactttta ccacgcacgg ttagcggtac
    21961 tgaccattta tcatctttac cctgaatgtc ctgcaaactg acccaagcca cgggatcggc
    22021 ctgaccgaca attttttgat catctgccca acgcgccacg cgattttgtt gataatcacg
    22081 ttttacgctc gcggcaattc cggcagcatc cagaccttca cactttggga aagtgaccga
    22141 cttgctggtt tcattattgg cagcgaaaac tgatgcgcag gcagaaacca acaacagccc
    22201 taaaaacgcc cctctttttt tcatgttttt ctccatagca caatgattca ggagaaagca
    22261 tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg tgtgaggcat cttcctcttc
    22321 ggattcagca ccgacgacct gtggggacgg taatttgccg gttttcagaa tggtgctcag
    22381 cacatctttt tgttctgcca gccatagcga aagggcttca cgttgctcgt cttccatttg
    22441 cactggagaa tttgtcagcc aggtgtcaag cagatccgcc gtatcaagca ttttatcata
    22501 agcatcggct tcctttttgc tggtaaacga cattttctct tcgccctcac gaatgactac
    22561 gtatttaact tcaaccgcca tttgcagcct ctcataataa ctgtgatttt atacagtata
    22621 tttcttttcg gttgagaaat caacatcagc aataaagaca cacgcaaacg ttttcgttta
    22681 tactgcgcgc ggaattaatc aggggatatt cgttatgacg ttattaggca ctgcgctgcg
    22741 tccggcagca actcgcgtga tgttattagg ctccggtgaa ctgggtaaag aagtggcaat
    22801 cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat cgctattccg acgcaccagc
    22861 catgcatgtc gcgcatcgct cccatgtcat taatatgctt gatggtgatg cattacgccg
    22921 tgtggttgaa ctggaaaaac cacattatat cgtgccggag atcgaagcta ttgccaccga
    22981 tatgctgatc caacttgaag aggaaggact gaatgttgtc ccctgcgctc gcgcaacgaa
    23041 attaacgatg aatcgcgagg gtatccgtcg cctggcggca gaagagctgc agctgcccac
    23101 ttccacttat cgttttgccg atagcgaaag ccttttccgc gaggcggttg ccgccattgg
    23161 ctatccctgc attgtaaaac cggtgatgag ctcttccggc aaggggcaga cgtttattcg
    23221 ctctgcagag caacttgctc aggcatggga atacgctcag caaggcggtc gcgccggagc
    23281 gggccgcgta attgttgaag gcgtcgttaa gtttgacttc gaaattaccc tgctaaccgt
    23341 cagcgcggtg gatggcgtcc atttctgtgc accagtaggt catcgccagg aagatggcga
    23401 ctaccgtgaa tcctggcaac cacagcaaat gagcccgctt gcccttgaac gtgcgcagga
    23461 gattgcccgt aaagtggtgc tggcactggg cggttatggt ctgtttggtg tcgagctgtt
    23521 tgtctgtggt gatgaggtga ttttcagtga ggtctcccct cgtccacatg ataccgggat
    23581 ggtgacgtta atttctcaag atctctcaga gtttgccctg catgtacgtg ccttcctcgg
    23641 acttccggtt ggcgggatcc gtcagtatgg tcctgcagct tctgccgtta ttctgccaca
    23701 actgaccagt cagaatgtca cgtttgataa tgtgcagaat gccgtaggcg cagatttgca
    23761 gattcgttta tttggtaagc cggaaattga tggcagccgt cgtctggggg tggcactggc
    23821 tactgcagag agtgttgttg acgccattga acgcgcgaag cacgccgccg gacaggtaaa
    23881 agtacagggt taaacccggc aaaaaaacgc tacaaaaatg cccgatcctc gatcgggcat
    23941 tttgactttt acagcttagc gccttctaca gcttcacgcg ccagcttagt aatgcgatcg
    24001 taatcgcccg cttccagcgc atctgccgga accagccagg aaccaccgat gcacagcacg
    24061 cttttcagcg ccaggtagtc acggtagtta gccggagaaa taccacccgt cgggcagaaa
    24121 cggacctggg agaacggacc cgcgatcgcc tgcagggctt tcacgccgcc gttagcttca
    24181 gccgggaaga atttgaactc tttcaaaccg tagtccatac ccagcatcag ttcggaaaca
    24241 gtgctgatcc ccggaattag aggaatagtc ccttcggtag cagctttcag cagcggctcg
    24301 gtcagacccg ggctaattgc gaactgtgca cccgcttcag tgacttctgc cagctgctgt
    24361 ggattcagca ccgtaccggc acccacaatc gcttcaggca cttctttggc gatagcacgg
    24421 atagcgtcaa ctgcacactc ggtacgcaga gtcacttcca gaacgcgcac cccaccagca
    24481 accaacgctt ttgccatcgg cactgcgtgt tccagttttt ttaccacgat aaccggtaca
    24541 accgggccgg tggtcaggat tgattctgca cttgttttcc agtttttcat cagagttttc
    24601 tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac aggttgcgcc ctgttcggca
    24661 ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc ctgtccccac gcgtgacgcg
    24721 ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg cttcgtctac cagcagcgtc
    24781 agttcgcctg tctgtccatt cacacgaatg atgtccccgt cgcgcacttt tgccagcagc
    24841 ccgccatcgt aggcttctgg tgttacgtgg atagctgacg gcactttacc tgaagcgccg
    24901 gagagtcgtc catcggtaac taacgcaatt ttgaaacacc ggtccaataa tacaccaagt
    24961 ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt ttggcccctg atgacggaca
    25021 acaacgacac aatcgcggtc cagcaaaccc gcttcaaagg ccggcataac gtcatgctgg
    25081 ctttcaaaaa caaccgctgg cgcttcaatc acctggttct caaccggcac ggcagaggtt
    25141 ttcataaccg cacggcccag gttaccgctt aacacttttg tcccaccatg atgagagaaa
    25201 ggttgttcga aggaagcgat cacattgctg tcgagtgatt tttccgcccc ttcccgccag
    25261 tccagttcac cattattcag ccatggttca agggtataac gagacagacc aaagcctgcc
    25321 accgtattga catcttcatg cagcaggcct gctttgagca gttcacgcac cagaaccggt
    25381 acgccacctg ccgcctggaa gtggttaata tcggccggac cgttcgggta gagacgtgcc
    25441 atcagcggta caacatcaga aaggtcagag aagtcatccc agttaatctg aataccggcc
    25501 gcgcgcgcca tcgccaccag gtgcatggtg tggttagtgg aaccaccggt cgccagcagt
    25561 gcaacgatac cgttcaccac cactttctca tcgatcatct taccgatcgg catccattca
    25621 ttaccattac cggtcatgcg tgtaacctga cgcgcagcgg cagcggtcaa agcatcgcgc
    25681 agcggagaat ccggatgaac aaaagaagag cctggcaact gcatccccat aaattccacc
    25741 accatctggt tggtgttggc agtaccgtag aaagtacatg ttcccggcgc atggtaagac
    25801 gcggcttctg actccagtaa ggccatgcgg tccactttac cttcggcata aagctggcga
    25861 atacgcactt tttctttatt tggcaaaccg cttgccatcg gtccagacgg cacaaacacc
    25921 gcaggcaaat gaccaaacga cagggctgcc atcgtcagac ccgggacaat cttgtcgcac
    25981 acaccgagga acagagcacc atcaaacatg ttatgggaca gccccaccgc cgcagacatc
    26041 gctatcactt cgcggcttag cagcgacaat tccattccat cctgcccctg ggtgacacca
    26101 tcacacatcg ccggaacacc gcccgcaacc tgaccaaccg cattcgcttc atgcagggct
    26161 ttacgaatga tttctggata gtgttcataa ggctggtgcg cggagagcat gtcgttatag
    26221 gaggtgatga tggcgatatt gttacgcaac atgcttttca aagaggcttt gtcttctggc
    26281 tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca actgcgaacg atgaacggtc
    26341 gacgttttcg cttgttctat ccgggcgaga taagcagagc gagtctcgcg cgaacgttca
    26401 atgattcgat ttgttacgcg taacaattgt ggattcataa aggctcctga aattgagttg
    26461 tcagagcagg atgattcaca acgcgtttca ttcagaggat ttatgagtga aacgcctgta
    26521 accggagctc atagggcaaa aacgtttcag tcagtgtaat aaaaaaagcc tcgtgggtga
    26581 atccgcacga ggcctgaaag tgtaaaaatt gttctacaat ctgtgcaaga tcatgttacc
    26641 ggtaaaataa ccataaagga taagcgcaga tattactcaa actcattcca ggaacgacca
    26701 tcacgggtaa tcatcgccac cgaggcaacg ggtccccagg ttccggcctg atacggtttc
    26761 ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt ctacccattt ccaggcttct
    26821 tccacttcgt cgcgacgtac aaacagtgcc tgaataccac gcatggtttc cagcagcaaa
    26881 cgttcatagg catccgccag atgcgtctga ttaaaggttt ctgaatagct cagatccagc
    26941 ttggtgattt gcaggttatg tttgtggtca aggccaggaa ctttattcag tacctggata
    27001 tccacgcctt catcaggttg cagacggata gtcagtttat tctgcggcag atcctgccac
    27061 gactctttaa acagattcag ttcaggtgtt ttgaaataga ccacgacttc agaacatttg
    27121 gtcggcagac gtttaccagt acgcaggtag aatggcacac cggcccagcg ccagttatca
    27181 atgtcgacgc ggatcgccac gaaggtttct gtattgctgc tcttgttcgc gccctcttct
    27241 tccagatatc ccggcacttt tttgccctgg gcgaagcccg cagtatattg cccgcgtacg
    27301 gttttttcgc gtacgttgta gcggtcgatg cggcgcagag acttcagtac tttcactttt
    27361 tcatcgcgga tgctgtctgc gctcaggtca gacggcggag acatcgcaat catgcaaaga
    27421 atttgcagca ggtggttctg gatcatgtcg cgcatctgac cggctttatc aaaatagccc
    27481 cagcgccctt cgatccccac ttcttctgcc acggtaatct caacatgatc aatggtgcga
    27541 ttgtcccagt tattcacaaa cagggagtta gcaaaacgca gcgccaacag gttcagcacc
    27601 gtttctttac caagatagtg gtcgatacgg taaacctggc actcctcgaa gtattcgcca
    27661 acctgatcat tgatttcctg cgaggtcgcc agcgacgtcc ccagcggttt ctccatgact
    27721 acgcgtgccg gtttagcatt cagttttgcc tcgccaagcc ctttgcaaat tgcgccaaaa
    27781 gtgctgggcg gcatggcaaa gtagttaatg gtgatacgat ttttttgatc cagcatcgcg
    27841 ccgagacggc tgaatgcagc agtgtcattg acatcgagat tacaaaaatc cagacgtgca
    27901 ctcagggtgt cccataaacc ttcatcaatg gtttctttca tgaaagtttc gagcgcctcg
    27961 cggacaactt tggtatatgc cgctttatcc cagtcagcac gccctacgcc gataatccgg
    28021 gtgtccgggt tgagctgacc ggctttttcc agttgataca gggaaggcag caatttacga
    28081 cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac aggcctgggc tgtttgcgtt
    28141 accgccatgt cattctcctt aagttaacta acccggtact taagccaggg tatacttgta
    28201 attttcttac ggtgcactgt actgctttta cgagcttgcg aaaactgtaa acgcttatcc
    28261 acccgtgcga ttacgggaaa agcgcgcaaa gtgcggcaaa actgataaaa aaatcatcgt
    28321 tttccattgg gtaaaaatct gacactgatc atgttatgaa aaaaaataac aactttttta
    28381 tctgcttttg tcattaacac ggcacacagg cgtaatatct gacaaaactg catttcgatt
    28441 tctttcagtg cggaaatcgt cattacccgt gagtctcttt acatcatgaa tatgctggaa
    28501 aaaatccagt ctcagctgga acatttgagc aaatcagagc gcaaagttgc cgaggtcatt
    28561 ctggcttcgc ccgataacgc gatccattcg agtattgctg ctatggcact ggaagccaat
    28621 gttagcgaac cgacggtgaa tcgtttctgt cgcagcatgg acacacgcgg ttttcctgat
    28681 tttaaacttc atctggcaca gagtctggcg aatggcactc cctatgttaa tcgcaatgtc
    28741 aatgaagatg acagcgttga atcatacaca gggaaaatat ttgagtccgc aatggcaacc
    28801 cttgatcatg tccgtcattc actggataaa tctgccatca accgcgccgt cgacttgctc
    28861 actcaggcaa aaaaaatcgc ctttttcgga ttaggctctt cagccgccgt tgcccacgat
    28921 gcgatgaata agttctttcg ttttaatgtt ccggtggtgt actccgatga tatcgtgctg
    28981 caacgcatga gttgtatgaa ttgtagcgac ggagacgtgg tggtgctgat ttctcacact
    29041 ggaagaacaa aaaatctggt cgagctggcg cagctggctc gcgaaaacga cgccatggtg
    29101 attgccctca cctctgcggg taccccgctc gcccgggaag caacgctggc aattaccctc
    29161 gacgtaccgg aagatactga catttatatg cccatggttt ctcgacttgc acagctgacc
    29221 gtgatagatg tgctggcgac aggatttact ttgcgacgcg gtgcaaaatt cagagataac
    29281 ttgaagcggg tcaaggaagc gctgaaggaa tcgcgttttg ataagcagtt acttaattta
    29341 agtgacgatc gctaaaaacg actgtcactg tcctaatctt atacgacatc cgaatgagat
    29401 taatttatcg ccatcgcggc gttatttcat tcggatttca tgttcaagca acacctggtt
    29461 gtttcagtca acggagtatt acatgtccag aaggcttcgc agaacaaaaa tcgttaccac
    29521 gttaggccca gcaacagatc gcgataataa ccttgaaaaa gttatcgcgg caggtgccaa
    29581 cgttgtacgt atgaactttt ctcacggctc gcctgaagat cacaaaatgc gcgcggataa
    29641 agttcgtgag attgccgcaa aactggggcg tcatgtggct attctgggtg acctccaggg
    29701 gcccaaaatc cgtgtatcca cctttaaaga aggcaaagtt ttcctcaata ttggggataa
    29761 attcctgctc gacgccaacc tgggtaaagg tgaaggcgac aaagaaaaag tcggtatcga
    29821 ctacaaaggc ctgcctgctg acgtcgtgcc tggtgacatc ctgctgctgg acgatggtcg
    29881 cgtccagtta aaagtactgg aagttcaggg catgaaagtg ttcaccgaag tcaccgtcgg
    29941 tggtcccctc tccaacaata aaggtatcaa caaacttggc ggcggtttgt cggctgaagc
    30001 gctgaccgaa aaagacaaag cagacattaa gactgcggcg ttgattggcg tagattacct
    30061 ggctgtctcc ttcccacgct gtggcgaaga tctgaactat gcccgtcggc tggcacgcga
    30121 tgcaggatgt gatgcgaaaa ttgttgccaa ggttgaacgt gcggaagccg tttgcagcca
    30181 ggatgcaatg gatgacatca tcctcgcctc tgacgtggta atggttgcac gtggcgacct
    30241 cggtgtggaa attggcgacc cggaactggt cggcattcag aaagcgttga tccgtcgtgc
    30301 gcgtcagcta aaccgagcgg taatcacggc gacccagatg atggagtcaa tgattactaa
    30361 cccgatgccg acgcgtgcag aagtcatgga cgtagcaaac gccgttctgg atggtactga
    30421 cgctgtgatg ctgtctgcag aaactgccgc agggcagtat ccgtcagaaa ctgttgcagc
    30481 catggcgcgc gtttgcctgg gtgcggaaaa aatcccgagc atcaacgttt ctaaacaccg
    30541 tctggacgtt cagttcgaca atgtggaaga agctattgcc atgtcagcaa tgtacgcagc
    30601 taaccacctg aaaggcgtta cggcgattat taccatgacc gaatcgggtc gtaccgcgct
    30661 gatgacctcc cgtatcagct ctggtctacc aattttcgcc atgtcgcgcc atgaacgtac
    30721 gctgaacctg actgctctct atcgtggcgt tacgccggtg cactttgata gcgctaatga
    30781 cggcgtagca gctgccagcg aagcggttaa tctgctgcgc gataaaggtt acttgatgtc
    30841 tggtgacctg gtgattgtca cccagggcga cgtgatgagt accgtgggtt ctactaatac
    30901 cacgcgtatt ttaacggtag agtaagtacg ttgccggata cggcgaaaac gccacatccg
    30961 gcctacagtt caatgatagt tcaacagatt tcgaatattc tgaagcaaac ttgaacttat
    31021 catcaggcga aggcctctcc tcgcgagagg cttttttatt tgatgggata aagatctttg
    31081 cgcttatacg gctggatttc gcccggtttg cgagttttca gcaattttag tatccaggtg
    31141 tattgttctg gtcgcggacc aacaaaaatc tcgacttctt cattcatccg ccgcgcaatc
    31201 gtatgatcat ccgcctctaa cagatcatcc atcggtgggc gcacctgaat cgtcagacgg
    31261 tgcgtcttgc catcataaat cggaaacagc ggaacaacgc gcgcacggca cactttcatc
    31321 aaacgaccaa tcgcgggcaa cgtcgcttta taggtggcaa agaaatcaac aaattcgctg
    31381 tgctcagggc catgatcctg atcgggtaaa taatatcccc agtagccctg acgtaccgac
    31441 tggatgaatg gtttaatacc atcatttctc gcatgcagac gaccgccaaa acgacgacgc
    31501 accgtgttcc agacataatc aaaaaccggg ttgccctgat tatggaacat cgctgccatt
    31561 ttctgccctt gcgaggccat cagcatggca ggaatatcga cggcccaacc gtgcggcacc
    31621 agaaagataa ctttctcgtt attacgccgc atctcttcga tgatctccag cccttgccag
    31681 tcaacgcgcg gctgaatttt ctccggcccg cgtattgcca actcagccat cattgccatc
    31741 gcttgcggcg cggtggcaaa catctcatca acaatcgctt cgcgttcagc ttcactacgt
    31801 tctggaaagc agagcgacag attgattaac gcacgacggc gtgagctttt tcccagtcgt
    31861 ccggcaaaac gtcccagccg tgccagaatg ggatcacgga actttggcgg cgttaaagcg
    31921 atacccgcca tcgctgctac gcccagccat gctccccagt agcgcgggtg gcgaaaggat
    31981 ttatcaaact caggaatgta ttcgctatta ttttttttcg tttccatgct tttccagttt
    32041 cggataaggc aaaaatcaat ctggtgatag tgtagcggcg caacttgccc cgcaccaaat
    32101 aaaaaaagcc ggtactgact tcgtaccggc tgcgaatgga tgttaattaa tcaaaccgta
    32161 gctgcggcac aatctctttg gcctgcgcca ggaattcgcg acgatcggag ccggtcagcc
    32221 cttcggtacg cggcagtttt gccgtcagcg ggtttacggc ctgctggttt atccatactt
    32281 catagtgcaa atgcggcccg gttgaacgtc cggtattacc ggaaagcgcg atacggtcgc
    32341 cacgtttcac cttctgtccc ggtttcacca gaatcttgcg caagtgcata taacgcgtgg
    32401 tgtagctgcg accatgacga atagccacat aataacctgc tgcgccactg cgtttggcaa
    32461 ccaccacttc accgtcaccc actgaaagca ctggcgtacc ctgcggcatg gcgaaatcaa
    32521 cacctctgtg tggtgcaacg cgaccggtca ccggattagt acgacgcggg ttaaagttag
    32581 acgagatacg gaactgtttc gccgtcggga atcgcaagaa tcctttcgcc agaccagtac
    32641 cgttacgatc gtagaatttg ccatcttcag cgcggatagc gtaataatct ttaccttctg
    32701 aacgcaaacg tacgcccagc agctggcttt gctcacgttt accatcaagc atttctcgag
    32761 acattaatac cgcaaattca tcgccttttt tcagtttgcg gaaatccatt tgccactgca
    32821 tggctttaat cactgcgctc acttcggcgc tggttaaacc ggcgtttctg gcgctggcaa
    32881 caaagctccc cccgacggta cctttcagca gattgttaac ccactctcct tgctgcattt
    32941 cgctggtcat tttaaaaccg ttagcggcag tacggtcata ggttcgggtt tcacgacgag
    33001 acacttccca ggtgaggcgc tgcagttcgc cgtccgcggt taatgtccag gagagttgtt
    33061 gaccgatttt caggttacgc aattctttgt cggcagcagc cagttgggtg atatcaccca
    33121 tatcaatacc atactgattg agaatgctgc ttagcgtatc gccagtggaa acaacatatt
    33181 catgcacgcc cgcttcaccg gcgattttgt catccagttc gtcctgagga atggcttcat
    33241 cttcttgtgc agcttgatca atcggctcac tggcttcagg taagagcgaa cgaatttcgt
    33301 tctgttccag ctcaatggtt ttgacaattg gcgtggcatc gcggtgataa acatagggcc
    33361 gccagacagc gacggccaga gtaagaacgg tgagcgaccc caacataacg cggtgtggtc
    33421 gcggtaaatt attaaacgcc agggcgacag agcgggctat ctgttgcacg taatcacttc
    33481 ctcattaatc tcctttcagg cagctcgcat actggttggc taattgactc aggaattctg
    33541 aatagcttgt tttacccagt ttgatattcg tccccagggg atccaacgtt cccatacgaa
    33601 cggatgtccc tcgtgcgacg ctctcaacga ccgctggcct gaactgtggc tcagcaaaaa
    33661 cgcaggttgc tttttgctca accaactgtg ttcttatttc atgtaaacgc tgcgcgccag
    33721 gttgaatctc agggttaacg gtaaaatgac caagcggtgt cagtccaaac tgtttttcga
    33781 aatagccgta agcatcgtga aaaacgaaat aacctttccc cttgagcggc gcgagctcgt
    33841 taccaacctg cgtttcggtt gaggctaatt gtgcctcaaa atccttcagg ttggcgtcaa
    33901 gtttggctcg actttgcggc ataagttcca ctaattttcc atggattgca accgctgtag
    33961 cccgcgctat ctctggggaa agccaaagat gcatgttgaa atcgccgtga tggtgatctt
    34021 cgtcactttt ttccgcgtgg tcgtgatcat catcatcgcc gtgaatactt ttcatcagta
    34081 gcggtttcac atcttcaagc tgcgcaatcg ttacctgctt cgcttctggt aatttgctta
    34141 ccggtttttg cataaacgct tccatctccg ggccaaccca aacgactaag tccgcgttct
    34201 gtaagcgttt tacatccgat ggacgcagtg aataatcatg ttctgaagcg ccgtcaggca
    34261 gtaaaacctc cgtttctgtt acaccatcag caatggcaga agcgatgaac ccaacgggtt
    34321 taagcgaagc gacaacggcg gcatctgcgg cctgtgttgc acctccccag agagcggcgg
    34381 ataatgctgc gaaaagaagc gtttttttat gtaacataat gcgaccaatc atcgtaatga
    34441 atatgagaag tgtgatatta taacatttca tgactactgc aagactaaaa ttaacatgac
    34501 aagtctggtt tccctggaaa atgtctcggt ttcttttggc caacgccgcg tcctctctga
    34561 tgtgtcgctg gaacttaaac ctggaaaaat tttgacttta cttgggccaa atggcgcagg
    34621 taagtcgaca ctggtacggg tagtgctcgg gctggtaaca cccgatgaag gggttatcaa
    34681 gcgcaacgga aaactgcgca tcggctatgt accgcagaag ctgtatctcg acaccacgtt
    34741 gccactgacc gtaaaccgtt ttttacgctt acgccctggt acacataaag aagatatttt
    34801 gcctgcactg aaacgtgtcc aggccgggca cctgattaac gcaccgatgc aaaagctctc
    34861 tggtggcgaa acgcagcgtg tactattagc gcgagcattg ttaaatcgcc cgcaattatt
    34921 agtgctggat gaacccactc aaggcgtgga tgtaaatggc caggtggcgt tatatgacct
    34981 tattgaccaa ctgcgtcgtg aactggattg tggcgtttta atggtttctc acgatctgca
    35041 tctggtgatg gcaaaaaccg atgaagtgct gtgcctgaat caccacattt gttgttccgg
    35101 cacaccggaa gttgtttccc tgcatccgga gtttatttca atgtttggtc ctcgtggtgc
    35161 tgaacaactg ggtatctatc gccatcatca taaccatcgt cacgatttac agggacgaat
    35221 tgttttgcgt cggggaaatg atcgctcatg attgaattat tatttcccgg ttggttagcc
    35281 gggatcatgc tcgcctgtgc cgcgggtccg ctgggttcgt ttgtagtctg gcgtcgtatg
    35341 tcttatttcg gtgatacgct ggctcatgcc tcattacttg gcgtcgcgtt tggtttgttg
    35401 ctggacgtga atccattcta tgcggtaatt gccgttacgc tgctcctggc gggcggtctg
    35461 gtatggctgg agaagcgtcc acagctggcg attgacacgt tattagggat tatggcgcac
    35521 agtgccctgt cgctgggcct ggtggtcgtt agtctgatgt ctaacattcg tgttgatttg
    35581 atggcttacc tgttcggtga tttgctggca gtgacgccag aagatctcat cgctattgcg
    35641 attggcgtgg tcatcgtggt ggctattttg ttctggcaat ggcgcaattt gctgtcgatg
    35701 acgattagcc cggatctggc gtttgttgat ggtgtgaaat tacagcgcgt gaaattgttg
    35761 ttgatgctgg tgacggcatt gacgattggt gtagcgatga aatttgttgg cgcgttgatt
    35821 attacttcac tgctgattat tcctgcagct actgcgcgtc gctttgcccg tacgccggaa
    35881 cagatggctg gtgtcgctgt tttggtgggg atggtggcag taactggcgg tttaaccttt
    35941 tccgcgtttt acgatacgcc ggcgggtccg tcggtggtgc tatgtgcggc actgttattt
    36001 attctcagta tgatgaaaaa gcaggccagc taatctatcg ctgaacacat ttgtcggatg
    36061 cggcgcgagc gccttatccg acctacggtt cgctatctct ggtaggccgg ataagacgcg
    36121 aatagcgtcg catcaggcac actgccaatg tcggatgcgg tacgagcgac caatccgact
    36181 tacggcattt ctggcggcgt tatgccaaag tgattccacg cccgcgtcgt cgccatacgc
    36241 ccacgcggtg tacgctgcaa aaagccttgc tgaatcagat aaggctccag cacatcctca
    36301 atggtttcac gttcttcgcc aatagccgcc gccaggttat ccagacctac aggcccacca
    36361 aagaatttat cgattaccgc cagcaacaat ttgcggtcca tataatcaaa accttcagca
    36421 tcgacattca gcatatccag cgcctgagca gcgatatctg ccgagatggt gccatcgtgc
    36481 ttcacttcgg cgaaatcacg cactcgacgc agcagacggt tggcaatgcg cggcgtaccg
    36541 cgagcgcgac gagcaacttc cagcgcgccg tcatcactca tctcaagccc cataaagcgt
    36601 gcgctgcgac tgacgatata ttgcagatcc ggcacctgat aaaactccag acgttgcaca
    36661 ataccaaaac ggtcgcgcaa cggtgatgtc agcgaacctg cgcgtgtggt tgcaccaatc
    36721 agggtaaacg gcggcaaatc aattttaatg gagcgtgccg ccggaccttc accaatcatg
    36781 atatccagtt ggtagtcttc cattgccgga tacaacacct cttcaacaac tggcgataga
    36841 cggtggatct catcaataaa cagcacgtca tgcggttcaa ggttagtgag catcgctgcc
    36901 agatcgcccg ccttttccag caccggacca gaagtcgtgc gtaaattaac gcccatttca
    36961 ttggccacaa tgttggcaag cgtagtttta cccaaccccg gaggaccaaa aatcaacaaa
    37021 tgatcgaggg catcgccgcg cagtttcgct gctttgatga aaatctccat ctgtgaacga
    37081 acctgcggct gaccaacata ctcttccagt aatttagggc gaatggcgcg atctgctaca
    37141 tcttccggca aagtggtacc ggcagaaatc agacggtctg cttcaatcat cctttacctc
    37201 ataacgcggc gcgtagggct tcgcgaatta atgtttcact gctggcgtca gggcgagcga
    37261 ttttgctcac catgcggctt gcttcttgtg gtttatagcc cagcgccacc agcgcggcaa
    37321 ccgcttcttg ttcagcatcg tcggtcgccg ggctggcagg agacgtgagc accagatcgg
    37381 cggctggcgt aaagagatcg ccgtgcaaac ctttaaatcg gtctttcatt tcgacaatta
    37441 aacgctcggc ggttttttta ccaatgcccg gcagtttcac cagtgctccc acttcttcac
    37501 gttcaacggc attaacgaac tgctgcgctg acattccgga gaggatcgcc agcgccaact
    37561 tcgggccgac gccgttggtt ttgattaact ctttaaacaa cgtgcgctct tgcttattgt
    37621 taaagccgta cagcagctgc gcgtcttcac gcaccacaaa gtgggtgaaa acgatcgctt
    37681 cctgacccgc ttcagggagt tcataaaaac aggtcatcgg catatgcact tcatagccta
    37741 cgccgcccac ttcaattaac accagcgggg gttgtttttc aatgatgatg cctctgagtc
    37801 tgcctatcac atgacgctcc tgcgtaatga atcaaagata atgctgtatg ataaaaaaat
    37861 gctggataga tatccagcga aggatgaaga aaacttgcga ggcgtcccaa cgatctgaaa
    37921 aatgg
//
LOCUS       DAZFWP020000029        37563 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFWP020000029 DAZFWP020000000
VERSION     DAZFWP020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.029"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            277..1650
                     /gene="aroP"
                     /locus_tag="ACHK0Z_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             277..1650
                     /gene="aroP"
                     /locus_tag="ACHK0Z_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU4501388.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1693..2547)
                     /gene="ampE"
                     /locus_tag="ACHK0Z_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             complement(1693..2547)
                     /gene="ampE"
                     /locus_tag="ACHK0Z_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU4501389.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2544..3095)
                     /gene="ampD"
                     /locus_tag="ACHK0Z_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             complement(2544..3095)
                     /gene="ampD"
                     /locus_tag="ACHK0Z_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU4501390.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3183..4076
                     /gene="nadC"
                     /locus_tag="ACHK0Z_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             3183..4076
                     /gene="nadC"
                     /locus_tag="ACHK0Z_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU4501391.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4279..4719
                     /gene="ppdD"
                     /locus_tag="ACHK0Z_RS06705"
                     /old_locus_tag="pgaptmp_001341"
     CDS             4279..4719
                     /gene="ppdD"
                     /locus_tag="ACHK0Z_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU4501392.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4729..6114
                     /gene="gspE"
                     /locus_tag="ACHK0Z_RS06710"
                     /old_locus_tag="pgaptmp_001342"
     CDS             4729..6114
                     /gene="gspE"
                     /locus_tag="ACHK0Z_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU4501393.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6104..7306
                     /gene="hofC"
                     /locus_tag="ACHK0Z_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             6104..7306
                     /gene="hofC"
                     /locus_tag="ACHK0Z_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU4501394.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7341..8384)
                     /gene="guaC"
                     /locus_tag="ACHK0Z_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             complement(7341..8384)
                     /gene="guaC"
                     /locus_tag="ACHK0Z_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU4501395.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8540..8584)
                     /locus_tag="ACHK0Z_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             complement(8540..8584)
                     /locus_tag="ACHK0Z_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501396.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8609..9229
                     /gene="coaE"
                     /locus_tag="ACHK0Z_RS06730"
                     /old_locus_tag="pgaptmp_001346"
     CDS             8609..9229
                     /gene="coaE"
                     /locus_tag="ACHK0Z_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU4501397.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9229..9972
                     /gene="zapD"
                     /locus_tag="ACHK0Z_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             9229..9972
                     /gene="zapD"
                     /locus_tag="ACHK0Z_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU4501398.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9982..10179
                     /gene="yacG"
                     /locus_tag="ACHK0Z_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             9982..10179
                     /gene="yacG"
                     /locus_tag="ACHK0Z_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU4501399.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10279..10668)
                     /gene="mutT"
                     /locus_tag="ACHK0Z_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             complement(10279..10668)
                     /gene="mutT"
                     /locus_tag="ACHK0Z_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU4501400.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10728..13433)
                     /gene="secA"
                     /locus_tag="ACHK0Z_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             complement(10728..13433)
                     /gene="secA"
                     /locus_tag="ACHK0Z_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU4501401.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13495..14007)
                     /gene="secM"
                     /locus_tag="ACHK0Z_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             complement(13495..14007)
                     /gene="secM"
                     /locus_tag="ACHK0Z_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU4501402.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14238..15155)
                     /gene="lpxC"
                     /locus_tag="ACHK0Z_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             complement(14238..15155)
                     /gene="lpxC"
                     /locus_tag="ACHK0Z_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU4501403.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15256..16407)
                     /gene="ftsZ"
                     /locus_tag="ACHK0Z_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             complement(15256..16407)
                     /gene="ftsZ"
                     /locus_tag="ACHK0Z_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4501404.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16468..17730)
                     /gene="ftsA"
                     /locus_tag="ACHK0Z_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             complement(16468..17730)
                     /gene="ftsA"
                     /locus_tag="ACHK0Z_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU4501405.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17727..18557)
                     /gene="ftsQ"
                     /locus_tag="ACHK0Z_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             complement(17727..18557)
                     /gene="ftsQ"
                     /locus_tag="ACHK0Z_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU4501406.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18559..19479)
                     /gene="ddlB"
                     /locus_tag="ACHK0Z_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             complement(18559..19479)
                     /gene="ddlB"
                     /locus_tag="ACHK0Z_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4501407.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19472..20947)
                     /gene="murC"
                     /locus_tag="ACHK0Z_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             complement(19472..20947)
                     /gene="murC"
                     /locus_tag="ACHK0Z_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU4501408.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21001..22068)
                     /gene="murG"
                     /locus_tag="ACHK0Z_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             complement(21001..22068)
                     /gene="murG"
                     /locus_tag="ACHK0Z_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU4501409.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22065..23309)
                     /gene="ftsW"
                     /locus_tag="ACHK0Z_RS06795"
                     /old_locus_tag="pgaptmp_001359"
     CDS             complement(22065..23309)
                     /gene="ftsW"
                     /locus_tag="ACHK0Z_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU4501410.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23309..24625)
                     /gene="murD"
                     /locus_tag="ACHK0Z_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             complement(23309..24625)
                     /gene="murD"
                     /locus_tag="ACHK0Z_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU4501411.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24628..25710)
                     /gene="mraY"
                     /locus_tag="ACHK0Z_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             complement(24628..25710)
                     /gene="mraY"
                     /locus_tag="ACHK0Z_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU4501412.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25704..27062)
                     /gene="murF"
                     /locus_tag="ACHK0Z_RS06810"
                     /old_locus_tag="pgaptmp_001362"
     CDS             complement(25704..27062)
                     /gene="murF"
                     /locus_tag="ACHK0Z_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU4501413.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27059..28546)
                     /gene="murE"
                     /locus_tag="ACHK0Z_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             complement(27059..28546)
                     /gene="murE"
                     /locus_tag="ACHK0Z_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU4501414.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28533..30299)
                     /gene="ftsI"
                     /locus_tag="ACHK0Z_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             complement(28533..30299)
                     /gene="ftsI"
                     /locus_tag="ACHK0Z_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU4501415.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30315..30680)
                     /gene="ftsL"
                     /locus_tag="ACHK0Z_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             complement(30315..30680)
                     /gene="ftsL"
                     /locus_tag="ACHK0Z_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU4501416.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30677..31618)
                     /gene="rsmH"
                     /locus_tag="ACHK0Z_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             complement(30677..31618)
                     /gene="rsmH"
                     /locus_tag="ACHK0Z_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU4501417.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31620..32078)
                     /gene="mraZ"
                     /locus_tag="ACHK0Z_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             complement(31620..32078)
                     /gene="mraZ"
                     /locus_tag="ACHK0Z_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU4501418.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32680..33684)
                     /gene="cra"
                     /locus_tag="ACHK0Z_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             complement(32680..33684)
                     /gene="cra"
                     /locus_tag="ACHK0Z_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU4501419.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33766..33852)
                     /locus_tag="ACHK0Z_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             complement(33766..33852)
                     /locus_tag="ACHK0Z_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU4501420.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33864..34355)
                     /gene="ilvN"
                     /locus_tag="ACHK0Z_RS06850"
                     /old_locus_tag="pgaptmp_001370"
     CDS             complement(33864..34355)
                     /gene="ilvN"
                     /locus_tag="ACHK0Z_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4501421.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34358..36082)
                     /gene="ilvI"
                     /locus_tag="ACHK0Z_RS06855"
                     /old_locus_tag="pgaptmp_001371"
     CDS             complement(34358..36082)
                     /gene="ilvI"
                     /locus_tag="ACHK0Z_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU4501422.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36201..36245
                     /locus_tag="ACHK0Z_RS06860"
                     /old_locus_tag="pgaptmp_001372"
     CDS             36201..36245
                     /locus_tag="ACHK0Z_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501423.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36400..37344)
                     /gene="leuO"
                     /locus_tag="ACHK0Z_RS06865"
                     /old_locus_tag="pgaptmp_001373"
     CDS             complement(36400..37344)
                     /gene="leuO"
                     /locus_tag="ACHK0Z_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU4501424.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9057 a        10470 c         9171 g         8865 t
ORIGIN      
        1 atttttaatt caattaaaac gaatttaaat tcattctaca tattgagagg ggttgaggct
       61 gagctttaca aacggtttct ttttaagcaa ctcatcttca accatgcata aagcgggtgc
      121 attcgctgcc gcataccatt attcttgatc tgacggaagt ctttttgtaa caattcaaac
      181 ttctttgatg taaacaaatt aatacaacaa acggaattgc aaacttacac acgcatcact
      241 gcgtagatca aaaaaacaat caccgcacga ggtttcatga tggaaggtca acagcacggc
      301 gagcagctaa agcgcggcct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg
      361 atagggacag ggctattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt
      421 atcctgggtt acgccattgc tggttttatc gcctttctga tcatgcgtca gctgggtgaa
      481 atggtggtcg aagaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc
      541 agttttgccg gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg
      601 gctgagctga ctgccgtggg taaatacatt cagttctggt atccggaaat ccccacctgg
      661 gtttctgccg ccgtattctt tgtggtgatt aacgccatca acctgaccaa cgttaaagtg
      721 tttggcgaga tggagttctg gtttgccatt atcaaagtta tcgcggtggt agcgatgatc
      781 atcttcggcg gctggctgct attcagtggc aacggcggcc cgcaggcgac cgttagcaac
      841 ctgtgggatc agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg
      901 attatcatgt tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc agaagctgat
      961 aacccggagc aaagtatacc gaaagcaact aaccaggtta tctaccgcat cctgattttc
     1021 tatattggtt cgttagccgt tctgctctca ctgatgccgt ggacccgcgt taccgccgat
     1081 accagtccgt ttgtgctgat cttccacgag ttaggcgata cctttgtggc gaatgcgctg
     1141 aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc
     1201 cgtatgctgt ttggtctggc acaacagggt aatgcgccaa aagcgctggc gtctgtcgat
     1261 aaacgtggtg taccagtaaa taccattctg gtgtctgcac tggtaacggc gttgtgcgta
     1321 ctgattaact accttgcccc agagtccgct ttcggactgt taatggcgct ggtggtatct
     1381 gcactggtaa tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag
     1441 caggaacaag gcgtggtaac tcgcttccct gctctgcttt atccgctggg taactggatc
     1501 tgcctgctgt ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt
     1561 tcggtatacc tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag
     1621 aaaaccgcca aagccgtaaa agcgcattaa tctctctacg ccctcacccg tacagggtga
     1681 gggcaataat ctttacacca acgccccgta aatcgtcagt agtgcaatca ccaccacgac
     1741 cacgaacgag gttttcttcg ccattgaaac cgctgccttc ggcgtctcca ccttatcgac
     1801 atgcggttca cgcgccagag agaactgcgc cagacgcgtt aacacctgat actgcgaagt
     1861 atggaaatca cccagcgaag caaaccaggc cggtaacgct ttctcaccat gaccgatcaa
     1921 ggcatatacc acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc
     1981 aatgccggac tgtaaacgat gatgcggcgt ctgatatcgc gccagccagt attgccatgc
     2041 acgcaaaaac gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca
     2101 gaacagcggt gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg
     2161 caaatactca cgttcgtcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc
     2221 catcgtggca cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg
     2281 aagacgaact ttacctgcgc caatacacag caaaccaatc agcagccaca ccagtagcgt
     2341 gggaacgttg aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc
     2401 aataatggtc atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc
     2461 ttcaagacga tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa
     2521 caccagtaag gttgtaaata gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt
     2581 gcccaatcaa atgcaggacc gggatcggtt ttccgatccg gcgcaatatc acaatggccc
     2641 gtcatgtttt tagcgatatc cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc
     2701 tgttgatact gcgcatcggt atacgccagc gtatcggtgc cttcaagctc aatcccaata
     2761 gaaaaatcat tgcagcgttc gcgcccctga tactgagaga ctcccgcatg ccatgcacgt
     2821 ttatcgaaag gaacatactg gactatttca ccatcacggc gaatcaaaca gtgagcggag
     2881 acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg
     2941 aataatgcgt cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc
     3001 accagcaggg tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg
     3061 cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct
     3121 aatacccggt tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta
     3181 acatgccgcc tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta
     3241 atctcgatat ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg
     3301 atgccaacaa tgatattacg gcaaaacttt taccggaaaa ttctcgctct catgccacgg
     3361 tgatcacccg cgagaatggc gtcttttgcg gcaaacgctg ggttgaagag gtgtttattc
     3421 aactggcagg cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaatg
     3481 ccaatcaatc cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca
     3541 ctgcgcttaa ttttgtgcaa accctttcag gagttgccag taaggtacgc cactatgtcg
     3601 aattgctgga aggcaccaac acgcagttgt tggatacgcg caaaacctta cccggcctgc
     3661 gttcagctct gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt
     3721 ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg
     3781 tcgaaaaagc gtcctggctg cacccggatg cgccagtaga agtcgaagta gagaatctgg
     3841 aagaacttga tgaagccctg aaagcaggag ccgatatcat catgctggat aacttcgaaa
     3901 cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg
     3961 gcaacgtcac tgacaaaaca ctgcgtgaat ttgccgaaac gggcgtggac tttatctccg
     4021 tcggtgcgct aactaaacac gtacaagcac tcgacctttc aatgcgtttt cgctaatagt
     4081 tgacagattt atccgctcca tcgcgggcgg ataatctccc cttccccaac tttcttcgta
     4141 acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg aatctgcttt
     4201 cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta gcgccaacca
     4261 aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga actgatggtg
     4321 gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg
     4381 cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag
     4441 ttgtgcgcgc tggaacatgg tggattagat acctgcgacg gtggcagcaa tggcattccc
     4501 tcgcctacca ccacccacta tgtttcagcc atgagtgtgg caaaaggcgt ggtgtcgctg
     4561 accgggcaag aaagtctcaa tggactaagc gtcgtcatga caccgggttg ggataacgca
     4621 aacggcgtca ccggctggac gcgcaactgc aatattcaaa gtgacagcgc attgcatcaa
     4681 gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca
     4741 cagctcactg ccctgtgtct gcgttatcat ggagtcttgc tggatgccag cgaagaggtg
     4801 gttcatgttg cggtagtcga tgcaccttcg catgagctac tggacgcatt gcatttcgct
     4861 accaccaaac gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt
     4921 cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa agcagagttg
     4981 ctaactcgaa cgttacaatc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca
     5041 gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggat
     5101 gtttcaccgg atgccggagt cgcattaacc gccagattaa aagtgctggg aaacctggat
     5161 attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc
     5221 gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg
     5281 ttacagcagg tgagccaggc actggatgtc aacacgcttg gaatgcagcc gttacaactg
     5341 gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac tggccctaca
     5401 ggcagcggca aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt
     5461 aatatttgta gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa
     5521 atccatccgc gtgccggact cacctttcag ggcgtgttgc gtgcgttatt gcgccaggat
     5581 cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cagcagagat cgctattaaa
     5641 gcggcgcaaa ctggtcacct ggtgttgtct accctacaca ctaattccac ctgcgaaacg
     5701 ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc gcttacgctg
     5761 gtaatagccc agcgtctggt acgcaaactt tgcccacatt gtcgccggca gcaaggggag
     5821 cccatccaca ttccagacaa tgtatggcca tcgccgctgc cccactggca ggcacccggt
     5881 tgtgtacatt gctaccacgg tttttatggt cgtacggcct tatttgaagt tctgcccata
     5941 acgccggtca ttcgtcagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac
     6001 gcacgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt ggagcaaggc
     6061 ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcga gtaagcaact
     6121 ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga
     6181 gagccgtgct ttgctgctca tggcactaca gcaacagatg gttaccccac ttagcctgaa
     6241 gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca
     6301 actggcgacg ctactcaaag ccgggttaac gctttctgaa gggctggcac tgctggcgga
     6361 acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca
     6421 gggcattgct ttttccaatg ccttattacc ctggtcagag gtatttccgc cactctatca
     6481 ggcaatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc
     6541 gcgtcagcaa aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc
     6601 catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc
     6661 ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa cgcaggggat
     6721 catgacgctg gcagacttta gtggcgaatg gagctggctg ctggtgttgt tcggctttct
     6781 gctggcgata gccaataagt tgctgatgcg ccgaccgacc tggcttatag tgcggcagaa
     6841 attgctgtta cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat
     6901 ttttacgatt ctggcgctga cacaaagtgc aggcattact tttttgcagg gcgtagagag
     6961 cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga
     7021 tatcagtaac ggtcatccca tctggctggc gctaaaaaat accggggagt ttagcccgct
     7081 ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca tgttagacaa
     7141 cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact
     7201 ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg tggcgatgta
     7261 tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc
     7321 tccacaccag cgttggaaga ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca
     7381 cggataaatg tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa
     7441 gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc
     7501 agcttaacgg ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc
     7561 atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg
     7621 atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca
     7681 ccgccgccaa aggcttttgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc
     7741 attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag ctgcggataa
     7801 ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg
     7861 atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca
     7921 caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg
     7981 gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc
     8041 tgtttagttt tttcgaaatc cgcatcagac gtaccggtag aaaccatcac atgtttcagc
     8101 acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc
     8161 acagcagtca aaatatcaaa agaagccagc gcagaggcca tagaaaatgt acctacggtg
     8221 tccatatttg cggcgataat tggcacgccg gaccagctct gacctgaatg tttgaaggtg
     8281 aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg
     8341 agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg
     8401 ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg
     8461 cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa
     8521 atagtgagcg aacgtttcat caatttcgct ttcgtgcact catttttaac gttttgataa
     8581 tcatgatgaa ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat
     8641 tggcagtggc aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga
     8701 tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc
     8761 tgatcacttt ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg
     8821 cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgccctgc tgcatccgct
     8881 gattcagcaa gagacgcaac accagatcca gcaagctact tccccctatg tactgtgggt
     8941 tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga
     9001 tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca
     9061 tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga
     9121 cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc
     9181 acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag
     9241 gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg
     9301 attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc
     9361 cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg
     9421 aaagaacttg accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac
     9481 cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc
     9541 gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga
     9601 ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat
     9661 ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc
     9721 acccaggcac ttaccatggt gctggattta attcgccagt cggctccctt ccgtaaacaa
     9781 accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat
     9841 ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt
     9901 cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg
     9961 gcctgttgct aaggagtgaa aatgtcagaa actattacgg tgaattgccc aacctgcggg
    10021 aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag
    10081 ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt
    10141 tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga tgccgcacgt
    10201 aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc
    10261 gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt cattggctgg
    10321 cggaaaatca tcggcattaa gaccgactag cgacatccac tcaccgggtt gcccttcttt
    10381 accccacggc tccccttccc agctttcgac cagccagaac cacagagtta tatgcctgtc
    10441 cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa tcccgacttc
    10501 ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc
    10561 gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgcgtta taaagatttc
    10621 attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg tcttaaacct
    10681 ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta ttgcaggcga
    10741 ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt acgtcctact
    10801 ttgcgctcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc atcctgatgg
    10861 ctaagctgct gcatttgcgc taaacgctcg gcttccatac gacgctgttg ttccagctcc
    10921 tcaacctctt caggcatacg tacctggact ttgctcagcg tgctgataac ttcatatttt
    10981 aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc ctgcttcgga
    11041 tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat cgctgccagg
    11101 tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa gtgacgcatc
    11161 atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg cgccagaatg
    11221 cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc ggcaattggt
    11281 aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc ccacatttct
    11341 tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc ttcacgaatg
    11401 ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg ggagtaaatg
    11461 gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg aatgtcgaag
    11521 ttacgacttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca cgggtgttca
    11581 atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg gtcggaagca
    11641 aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc agcatccccc
    11701 tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg acgctcggta
    11761 ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg ccagtcggct
    11821 ttaatttttt caatttgctc tacggtcgga ttttccagcg cggcaacttc tgcctgccag
    11881 ctaccaccga gcacaatatc tgtaccacga cccgccatat tggtcgcgat agtcaccgca
    11941 gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt ggcgttcagg
    12001 acgttgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga tttttcgatg
    12061 gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat atcttcaatg
    12121 atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc tttacgaatc
    12181 attggacggt tggtcggaac aacgacggta tccagcttgt agatggagct aaattcgaaa
    12241 gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg gaagtagttc
    12301 tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc ttctttcgct
    12361 tccacagcct ggtgcagacc atcggaccag cgacggccct gcatggtacg accggtgtgt
    12421 tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcgcg ggtaaacagc
    12481 gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc cggagagtac
    12541 agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat cagcaccaga
    12601 ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc
    12661 tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat tttattcacg
    12721 cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct
    12781 tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt acgctgtaca
    12841 cgttcttcag ggctgaacgc catattgtcg cgcaggtagt caaagccgta ttcgttgttc
    12901 gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg catgcccggc
    12961 aggttgatac cgacagtcag gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca
    13021 cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt tagtgcgttc
    13081 aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg
    13141 cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg cataccaaag
    13201 acgcgcttac ttgcctcacg taccacggcg aaagcttccg ggatcagatt ttccagcact
    13261 tcgccttttt ccagacgtgc acgaaactct gcggttttcc ctttcagttc ttcgtcggag
    13321 agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg catccggcgc
    13381 agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag cataataaaa
    13441 tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt gttgttaggt
    13501 gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg gcgtgctgaa
    13561 ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag acggcgtgcc
    13621 ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg cctgaagagg
    13681 caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa gatgacggat
    13741 taccgtgcga atggcatgtt gatgccagta atcaacggaa tagttcgaat tcgggcggcg
    13801 tgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag gctcgtggtt
    13861 gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc
    13921 taaactcgcc gcaaccatcc ctaataagag atgcggccag aagtagcgtt taccaaactg
    13981 tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgtatt caaacgtatt
    14041 tagcgcacaa atcttatcat taaagcctgc agtccacagc acgaatgaag ataagttgtg
    14101 cggaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta taaaatttaa
    14161 aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc gaattttata
    14221 cgacagtata aatgtcgtta tgccagtaca gctgaaggcg ctttgaaggc caacggcagt
    14281 tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag gacagcctgc
    14341 agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc aataatattg
    14401 tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac aaattcgtct
    14461 tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat ggcacaatcg
    14521 aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg catgaaaccg
    14581 aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt catcgcatag
    14641 cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa
    14701 ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac agtctctttg
    14761 atgcgaacaa attttttggc gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat
    14821 acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac ttcgataaca
    14881 atgttatcga tgcccaagcc cgcgagagca gcattgaggt gctctacggt tgaaatccgt
    14941 acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga tttggcatcg
    15001 gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc ggtgttggcc
    15061 ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc
    15121 tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca aattacctat
    15181 ccaactgaag tgtactatac attcggcggg ccagtttagc acaaagagcc tcgaaaccca
    15241 aattccagtc aattcttaat cagcttgctt acgcaggaat gctgggatat ccagataatc
    15301 cggctctttc gcagtttgcg gcgcattgtc attcacgact ttagcaaccg gcttctgctc
    15361 ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct gctgaacctg
    15421 cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg tcgcaacaac
    15481 ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc
    15541 gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat ccagacgcag
    15601 gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt cgatatcttc
    15661 cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt caccgctcgc
    15721 cacgccagaa cccatcattg cgtagcccat ctcagacatt acggtgcgta cgtctgcaaa
    15781 gtccacgttc atcaaacccg gacgagtaat cagttcagcg ataccttgca cagcgccttt
    15841 cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc ccagaacttt
    15901 cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca gttcagtgat
    15961 cccctgctcc gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct tagtgacgac
    16021 agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg ctgcacctgt
    16081 accggtacca ccccccatac ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc
    16141 acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat tagcgccagc
    16201 gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg
    16261 cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatgc gctcgcgcac
    16321 catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc
    16381 attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg tcgcctgagg
    16441 ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac tattgagtcg
    16501 cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt caccgttaag
    16561 atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg gctcctgagc
    16621 ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac
    16681 gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc
    16741 tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact gcaatatctc
    16801 ttcgttgacc aggttgagca gctcggtata gcgcggctcg atcacctctg ccagggtctg
    16861 acgttgcaga ctccgcggcg gacgaccacc tacgctcggc acttccacgc tctcatcttt
    16921 tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg
    16981 cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat aaggaattac
    17041 cttagtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac caccaccgat
    17101 atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat aacttgatgc
    17161 cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat
    17221 gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca tccgcacgcc
    17281 cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact cttgcgggat
    17341 cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt
    17401 ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa tttcattctg
    17461 gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac aatctgccat
    17521 caattctgcc tggtcaatgg cgcgttgtac gcacttgacc acggattcga ggtcgttcac
    17581 cccgccttta tccataccac gtgacgggca gcttcccacg ccaatgatat tgaccatacc
    17641 gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaatct ccagtcctac
    17701 taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg attttgttgc
    17761 tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga gtcataacgc
    17821 aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa aaccggataa
    17881 agttctacaa agcgagccaa acgtttcatc gtatcgcccc ggccaagatt gagcttaata
    17941 tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta
    18001 aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac ttcattggcg
    18061 ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc tggcggcacg
    18121 ctgaaggtgt ttccttccgc gtctaccata tgttgatcat tccaccgcgc aatcggcaca
    18181 tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct cacctgctta
    18241 atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcttg ggtcataaag
    18301 gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg cgtgtaatgg
    18361 cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc catccagccc
    18421 aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa aaggatcccc
    18481 gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctatt tcgcgtgttc
    18541 agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca actgcgagaa
    18601 gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca tacccggtga
    18661 ggtattggct tccagcagat aaaactgtcc atcgctgtcc agcataacgt caatacgtcc
    18721 ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca aattggcctc
    18781 ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga gatacttcgc
    18841 ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa tttcttcacc
    18901 gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata cttcttcatc
    18961 gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta cttttgacat
    19021 tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca gagcagaaat
    19081 ttctgctaac tgcttatcgc tcaggccttt ttcaaactct gcgcgggtta acgctaccca
    19141 cggcgcgacc ggtaaaccgg caccttgcca cagcagtttg ctgcgtagtt tatccattga
    19201 aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct cgagcatgcc
    19261 ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc
    19321 catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg catcaatacc
    19381 gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt cccgctcagc
    19441 ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc tccggagttt
    19501 gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta ccagccccct
    19561 gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct acctgcgcag
    19621 ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga cacagcgaac
    19681 ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc atcaacaggg
    19741 tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac
    19801 ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc gccgctttaa
    19861 tggtggcgtc aacttccgtc gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc
    19921 ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg cgcccagtcc
    19981 cctggaagct ctccagcgca cgtaaaatag cctcgtcatc aattccctct tccgtagcaa
    20041 ccgcaaccgc agctgcggcg ttcagcgcgt tatgacgacc tggcgcattc agggtgacgc
    20101 gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc ctgcgggcca atctgctgat
    20161 aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agtcgtggtc tgacgtccca
    20221 ctcgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca cgaccgtaaa
    20281 acggcaggtt gtgcagaaaa ttaataaaag tctgttttaa attctcaaag tcgccctggt
    20341 aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt tgcagatgca
    20401 ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg
    20461 catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg agccccgctt
    20521 ctgcgtagat gctggaaacc atcgcggtgg ttgtcgtttt gccgtgcgtt cctgcaatgg
    20581 cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg atcaccggaa
    20641 tacgcgcttc atgagctgcg acaatttccg ggttatcggc agaaatggcg ctggaaacaa
    20701 cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc gtcgctccca
    20761 gattcattaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg atctgataac
    20821 cttcattggc cagaacttcg gcaataccgc ccataccggc accaccgatg ccgacaaaat
    20881 gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc aattgttgtg
    20941 tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc atcgctacaa
    21001 ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat ccggaatgga
    21061 tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc cggccagggt
    21121 gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg cgcccgcttt
    21181 ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg gcacaaacaa
    21241 cgccggtagt cctgccgcgg cgatttcact caccgttaaa gcaccggagc ggcaaacgac
    21301 gacatccgcc cacgcatacg ccgccgccat atcatcaata aattccgtca ctttatgctg
    21361 cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt taccgctctg
    21421 atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg tctggttaag
    21481 aatgcgtgcg ccctgggaac cacccactac cagcacacga accggacctt cacgtccagc
    21541 caaacgttgc tgcggcaacg gcagcgccag tacatcggta cgcaccgggt tacccaccac
    21601 ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtggcaa tcttcgccag
    21661 ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg gaatgcctaa
    21721 cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga gtaccacgtc
    21781 aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg
    21841 cgcagctatc agtgctttta taccttttcc acgcagacca gagatacgaa tgaaatcaat
    21901 ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg
    21961 aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc agtcccggga atacatgtcc
    22021 accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg tgaacctcgt
    22081 acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag catcatgatg
    22141 gctgtcgaca taatcagtaa gctcgaacca ccgtaactga tcagcggcaa tgtcagacct
    22201 ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa gctaaaccag
    22261 atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa cgctttgcgg
    22321 ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta aaagtgccag caccacaccg
    22381 acatacccca gttcttcgcc gataatggcg aaaataaagt cagtgtgcgc ttccggcaga
    22441 tactccagtt tttgtaccga gttacctaaa ccttgtcccc aaagttcgcc gcgaccaaac
    22501 gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca cgggttccag
    22561 aatgcggtaa cacggcggat acggtacggt tcggcgagta tcagcaacac aaccgctgaa
    22621 atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag gaacaacatc
    22681 gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc cagcagtaac
    22741 actgccaaca ccagaatcac gcccatcggt ttcaggaagc cgcgcaggtt attacgtact
    22801 tcgtcgcctt tacgcaccag atagttggcg atataacaaa acagcgacag ttttgtcagc
    22861 tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc tttaaccgag
    22921 ctacccacta ccagcacgat catcagcagg atgatagatc cgagcagcat cgtggcactg
    22981 tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat gaatgccagg
    23041 atcagataga cgccatcacg cttcgcgaag aagaacggat cgttggttaa gcgttgccct
    23101 atgggcattg acgccgaggt caccatgata aagccaatcg ccgcaaggcc gaaggtcagc
    23161 cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg cgagcccatc
    23221 acccagcctt ttagcgccgt ggagatccag accaggatac tgaatcctgg caggcgcggc
    23281 attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga cgggcaaact
    23341 cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggcc ggggagagca
    23401 gaaccatatc gcccggctga acacgcggag ccagcaagcg catcgcctgt tccatagttt
    23461 cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcaccgtca cgaccaaaac
    23521 agtacaaacg cacgttgtcg ccattcagat agcgcgccag tggactgaag tccgccgatt
    23581 taccatcgcc gcccagcaac aaatgcagcg tgccgtctac ctgcaagcca ttcaacgccg
    23641 cttccgtact gccgacgtta gtggctttcg aatcgttaat ccagcgtacg ccattgtgct
    23701 ccagcaccac ttcaaagcga tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc
    23761 tggcgcgcgg caaaccagct gcgtctgcca gtgccagtgc tgccagcgca ttggtgtagt
    23821 tatgctgccc ggaaagtttc atctctttca cgttcagcac tttctcgcct ttcactcgca
    23881 gccaggtttc tccctgctga tgattcaggt gatagtcacc catgttgacg ccaaagctga
    23941 cgcagcgttc atcagcaccg cgaatcggca ttgttaaggc atcatcagca ttgacgacac
    24001 aaactttcgc attttcgtaa atacgcaatt ttgccgcacg gtattgctgc aaaccaaacg
    24061 gatagcggtc catatgatct tcagtcacgt tcagaatggt cgccgccacc gcctgtaagc
    24121 tggaagtggt ttccagctgg aagctcgaca gttccagcac gtacagttcg cactcagcat
    24181 caagcagcat caacgctggc aggccaatat tgccacccac accaacgtta acccccgccg
    24241 ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg
    24301 ccacaatcgg tgcttgtgct tcgcggcaga acagctcgat atcgccaacg atttcgattc
    24361 cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga ctggcgacaa
    24421 tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt tctacggctt
    24481 cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac
    24541 cgcgagcgag gaaaaagtcc acgcaggaaa gcccagtgag gcccaggccg ataatgacga
    24601 catttttacc ctgataatca gccatgatta acgtaccttc agcgttgcca gaccaatcag
    24661 aaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg gccagccttt
    24721 cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa
    24781 ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc ccataatcac
    24841 cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac ctaacgccag
    24901 cgaacctaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca ggaagcccag
    24961 tcctgccccg actatcgccg tgcagacaat gaccagttcc ccggcgtgtc gcagatacgg
    25021 tatgtgtaaa tagctggcaa agttcatgtt gccggtcgcc cacgccacca gcgcaaaacc
    25081 accggcgaca aatacggtcg gcataattgc caggccgtcg agaccatcgg tcaggtttac
    25141 cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca accccagctg
    25201 cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc
    25261 aaggtacagg gcgaaggcga tacccagcgc aatgaccgac atccagaaat acttccaacg
    25321 agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa caaagccaat
    25381 aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg acgggtaagc
    25441 ccacagcagt acggagatca caatcgccgt caggatcata atcccgccca tggtcggcgt
    25501 accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc accacctgac caaaggaaag
    25561 tttttgcaaa tgagcaatca tacgcgggcc catccacaat gagatgaaca gcgcggtcag
    25621 caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg aataatattt
    25681 gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc gcgtactacc
    25741 tcttccatgg cggcactacg tgaaccctta actaaaatcg taattacctg ttgctcagca
    25801 atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc gccaacgccg
    25861 ctggcggtgc tgatagcatg gctttgttta cccacgctta acacgcggtc aataccagca
    25921 gcttttgccg cctcgcccac ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc
    25981 atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg aacagcggcg
    26041 gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt ttctgccagt
    26101 tggatgggga acagacggcc tggaacagct ttcagatttg ccagccccgc tttgatagca
    26161 tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt cgcaatattg
    26221 tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg tagggtaaat
    26281 tccgtaccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt ggcggcattg
    26341 ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag ccagtcgttg
    26401 ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat ttcacctttc
    26461 gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc caggttgttg
    26521 accagcgcag cttccgggcg agtcagactc acagtccagg ctatttcgcc ctgatggttc
    26581 gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt
    26641 acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca ctggcttaaa
    26701 atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag agcaaccacg
    26761 cgcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag acgcgtatcc
    26821 ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg
    26881 ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcgcc tttcagggca
    26941 acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc aagggtgata
    27001 tctgcacctt gcagttcacc gttgagaata tcggtaagtt ggctaagggt tacgctaatc
    27061 atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat
    27121 tgccaacaat ctggtaatcc tcatggcctt tgcccgcgac cagtaccaca tcattctctt
    27181 tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact ttggcatgtc
    27241 cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct tcggtacgcg
    27301 ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg cccatcagtg
    27361 gacgcttacc tttatcgcga tcgccaccac agccaaagac gcaccacagc ttgcccgcac
    27421 agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca
    27481 ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa accggttgca
    27541 gacgcgcggc ggtttttaac agatcagcca gcgggtagcc aagtgccagc agtgtcgcca
    27601 gcgcgagcag caggttgctg acgttaaaag cgcccatcag atggctttca atttcgccat
    27661 cgccccaact tgaactaaag cgaatggtcg cgccgctgtc gtgatagttc acttcggtcg
    27721 ctttcaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat accgcaaccg
    27781 cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta acaatcgcct
    27841 gaccacaatg atgttcagaa taaagcagcc atttcgcggc ttcgtagtgt tccatatcac
    27901 catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgca aatttcaatg
    27961 ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa aacgtcgcgc
    28021 cctgatccac cagccccgcc agctcatgct gaacatcgac tgccgaaccg gtggtatttt
    28081 ctgtcgggat cactttcccc agcagaccgt taccaacggt gcccattacc gcgctggttt
    28141 cgccaagcag ttggctccac tgcgccagca gctgggtagt cgtggttttg ccgttggtgc
    28201 ccgttacgcc tacgagacgt aaattgtcag agggttcatg gtaaaagcgg cccgccagtg
    28261 cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc atttcacgga
    28321 tttcaccgtc ggtcgcctca cctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    28381 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    28441 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    28501 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    28561 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    28621 gtacgcaata cgccgcccat gatggcacca aagaccggcg ctgaaacggc gccgccgtag
    28681 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    28741 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    28801 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    28861 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggattcc
    28921 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    28981 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    29041 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    29101 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    29161 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    29221 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    29281 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    29341 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    29401 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    29461 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    29521 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    29581 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    29641 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    29701 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    29761 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    29821 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    29881 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    29941 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    30001 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    30061 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    30121 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    30181 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    30241 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    30301 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    30361 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    30421 tcaaggatca ggttgcgcca ttcaatatct aaagcatctc gctccagcac cagttgttcg
    30481 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    30541 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    30601 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    30661 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    30721 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    30781 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    30841 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    30901 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    30961 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    31021 gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc agttctttgg
    31081 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    31141 aacgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct gcggtttgta
    31201 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    31261 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    31321 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    31381 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    31441 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    31501 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    31561 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    31621 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    31681 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    31741 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    31801 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    31861 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    31921 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    31981 tgaccggcag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    32041 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    32101 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    32161 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    32221 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    32281 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    32341 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    32401 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    32461 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    32521 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    32581 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacgcgc
    32641 cagagggaaa ttcacctggc gcgtattttt gttcgcggct tagctacggc tgagcacacc
    32701 gcggcgatag agattacgtt taatgcgcgt taaaccaggt tttggcttac gcggttcgtc
    32761 caggcttgcc aggacaatct ccagcacacg ctctgcgaca tcgcggtgac gttgagccac
    32821 tgccagcacc gggcactgta agaagtcgag cagttcgtta tcgccaaagg tggcaattgc
    32881 caggtcagaa ggcagtttac catcgcgacg tagcgtgaca tccatcaccc cttgcaacaa
    32941 cgcaaacgac gttgtgaaca gcgcctgcgg catcggatgc gtttccagcc atttttcgaa
    33001 taactgggca gccgcctccc gctcatagct gttggcatac aggaaatgca cttcgcgcgg
    33061 atcatctttc caggcagtac ggaaaccttg ttcacgcagg aagctgacag aaagctccgg
    33121 cagcgcgcca agataaagca ccgtctcggc gggaaactta cgtaactctt ccgccagcat
    33181 ttcggcatca tcctgatcgg caccaaccac gctggtgaaa tgttcacgat cgagggcgcg
    33241 gtccagcgcg acaatcggga acgggtcgtt agcccagcgt tgataaaaag gatgctcagg
    33301 tggcaacgac gtcgaaacaa taatggcatc aacctgacgc tgtaaaaggt gctcaatgca
    33361 gcgcatttcg ttgtctggct gatcttctga gcaggcaatc agcagttgat aaccccgttg
    33421 ccgcgcctgg cgctcaagat agttagcgat gcgggtatag ctggtgttct ccagatcggg
    33481 gatcacaaga ccaatagaac gtgtgcgtcc agcacgaagc ccagctgcca cggcgttcgg
    33541 gtggtaattg tgctcacgca ccacagccat gactttttca acggttttgt cgctcacacg
    33601 gtattgcttc gctttgccgt taataacata gcttgcagtg gtccgcgaca ctcccgccag
    33661 ccgagcgatt tcatccagtt tcacaattgc cccttgcgta aaatgtaaaa accatagcca
    33721 ttgtactggt atgggttaaa ttcattaaca tctaagcgca gaaaattaac tgcggcaact
    33781 gcttttattc caggttccca ctgatttcgc aaaaaaagcc caacgtgaca tgttgggctg
    33841 caaattgcgc actgagatca ttctcaacgc attattttat cgccgcgcga aagtccgacc
    33901 acaccagagc gagcaacttc cacaattttc gccacatcgc gaatcgatgc taaaaatgca
    33961 tcaagcttat cgctggtgcc tgctaattga acggtataaa gcgagggggt gacatcgata
    34021 atttgcccac ggaatatttc cgtattacgt ttcacttcgt cacgcccgta accgctggcc
    34081 tgaattttca ccagcatgat ttcccgctca acatgcgcgc cctgccccaa ctcactcacg
    34141 cgcaagacat cgaccagctt gtgtaattgc ttttcgatct gctcaagcac tttttcatcg
    34201 cccacggtct ggatggtcat acgcgataat gtcggatcgt cggttggcgc aacggtcagg
    34261 ctttcaatgt tgtagccacg ctgggaaaaa aggccaatca cgcgggataa cgcgcctgat
    34321 tcattttcga gtaagactga taatatccgg cgcataatca ggttctctcc gttttgctta
    34381 accacatttc atccattccg cccccgcgaa tctgcatcgg gtagacgtgc tcactgccat
    34441 cgacggtaac atcaacaaac accaggcgat tattgcacac ctgttccagc gcctcgctaa
    34501 gtttgctttc cagctcatgc ggatgagaga tctggatccc gacgtgacca taggcttccg
    34561 ccagacggac gaaatcgggt agtgattgca tataagattg tgaatgacgg ccggaataga
    34621 tcatgtcctg ccactgtttc accatcccca gatagcggtt attgagattc accaccagta
    34681 ccggcaactc gtattgcaac gcggtagaca gttcctggat gttcatctga atactgccgt
    34741 cgccagtgac gcaaaccacg gtttcttctg gcaacgccat tttgacgccc agtgccgcag
    34801 gtaaaccaaa acccatcgtg ccgaggccac cggaattgat ccagcgacgc ggtttgtcga
    34861 atggataata aagtgcagca aacatctggt gctgcccgac atcggacgtc acgtaagcgt
    34921 ctcccttcgt caaccgccaa agagtctcga tcaccgcctg cggtttaatc ttttcactgt
    34981 gagtgtcata tttcaggcac tgacgagcgc gccactgttc aatttgctgc caccagtcgc
    35041 ggatctcatc cagtggttga tgggcggatt cttgcgacaa gagttcaagc atttgttcga
    35101 ggacctggcg agcatccccc acaatcggga tatccgcagt cacggtttta gaaatggaag
    35161 taggatcaat atcgatatgc agaacagtgg catttgggca gtactttgcc agattgttcg
    35221 tcgttcggtc atcaaatcgt accccgacgg cgaaaatcac atccgcgtta tgcatcgtca
    35281 tattggcttc gtaggtaccg tgcattccca gcatgcccag cgcctgacga tgcgttgccg
    35341 gaaacgcccc cagccccatc aatgaggaaa caacgggcag attcaacgcc tccaccgttt
    35401 ctttcaactg ctgatggcag cccgccatga ttgccccacc gcctacgtag acaaccggtt
    35461 tttttgccgc taccagcgtt tgcagagcgc gcttaatttg ccctttatgt ccggtagtag
    35521 tgggattgta agaacgcata ctgaccgact caggccagac atagggtaat ttgttcgccg
    35581 gattaagaat atctttcggt aaatcaacga ccactggtcc tgggcgacca cttgccgcca
    35641 gccagaaagc ctttttcagc acctgcggaa tgtcttccgt ttgcttaacc agaaaactgt
    35701 gtttaaccac cggtcgcgaa atccccacca tgtcgcactc ctgaaaggca tcgtaaccta
    35761 tcaacgaggt cgctacctgc ccggaaagga caactaatgg aatggaatcc atataagcgg
    35821 tggcgatgcc ggtaatcgca ttggtcgccc ctggacccga cgttaccagc acgacgccga
    35881 cttccccggt cgcgcgcgcc agaccatcgg ccatatgcac tgccgcctgc tcatgacgaa
    35941 ctaaaacatg atcgatacca cccacggtat gcaatgcatc gtaaatatca aggactgcgc
    36001 ctccgggata accgaatact tgtttaacgc cctgatcgat aagcgatcgg acgaccatct
    36061 cggctccaga caacatctcc atggcctgcc tcactgtttg acggaaaaaa tgtgtaaaac
    36121 acattaaccg tccgatcttg cttaagcaat tggcagcaag agacataaac gcagggtaat
    36181 aagaataaac aggaggaaag gtgaaaagaa acgataaaat cctccattac gaaggattaa
    36241 attgataata aatcggctga atcccacaac ttacagcaat ttctctattt ttttaattaa
    36301 tcctaaaaca gagagcccac acattcagaa taaaattcta cattcaataa aaaatacaga
    36361 ataaaccaga cattcatgcc tgacctattc tgcattcagt tagcgtttgc aaattgagac
    36421 taattgctct tccatccact gatggccttt atcgcgcccg gcagcttcat gccaggagag
    36481 ataacaggtt ctgctatttt gttttaacgg cagcggtaat acctgtaatt ctaaggattc
    36541 agcgaactct tcagccagcc aacgcggcgc aatagcgacc aaatgcgttt gcgacaccac
    36601 gctaagtacg ctcatcattg ccatgccctg atacgcgata ctggcttgct tatctaccgt
    36661 gtcataccaa ggttgactaa atgacgcgaa acgatcgagc gaaaccgccg catgttgttc
    36721 gttataaaca tcatgtttca gtaacgggcc cttaattgtt ggatgatttt tgctggctac
    36781 cagcaccatt tcatctttaa ataatggcac gctggtaaat tcaggacgat ggaagtcttc
    36841 ataactaatc acaaactccg tttcctgata acgcagctga tgttcagtgt tctgatttaa
    36901 tgaagacttg aacataacat gtatatttgg cgcaatctgc tcaatgtgat tataaatctg
    36961 cgaggtcaga atgctgtcta acgggctgca aacacaaaga tgaaatacac gttcactgct
    37021 cgcgggttca aaacctgaac caggcaattc attttgtact agttgcaatg cctgacgaac
    37081 tgagccaaaa agttgaaatg cgcgagcagt cggttgaata ccacggccat aacgaacaaa
    37141 aagctcgtca ttaaacatca ccttcaggcg tgcaacagcg ttactgaccg caggttgcga
    37201 catacccaga acatgagcgg cacgggtaat gttttgctcc tgcatcacgg catcgaaaac
    37261 ggttaataag ttgagatcga ccatgcgtag ctgtggcttg cttaactccg ccgtctctga
    37321 atgatctgtt tgtacctctg gcatacttaa ctccactgtc acacttaact ccctttccct
    37381 tattggaatg cagagaaata atcctgaaga atatgattta tcatgcatat aaaataagaa
    37441 aaagcaaaat gagtaaaatt cggaaaacat aaagatacgg gttctgataa aaacagaatc
    37501 cattgccgtg gaatgagtca tttaccacag cataataatc cataatattt ttgttgcgaa
    37561 aac
//
LOCUS       DAZFWP020000030        37216 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFWP020000030 DAZFWP020000000
VERSION     DAZFWP020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.030"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(22..1776)
                     /locus_tag="ACHK0Z_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             complement(22..1776)
                     /locus_tag="ACHK0Z_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HGU4501425.1"
                     /translation="MKLAPNVKQQSRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGDQQLSELDKLQIVPEGRKSVRIFRAGHLEPVMIKAIGQKLAAAGVQDA
                     NFYPEGMHVQEVENWREYLARERQNLSEGLFVELPVKQKAQLSQMADSERAQLLSDRF
                     DGVCVHPESEIVHVWRGGLWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLTTNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDKDLPEKVAAE
                     IPVIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVADFSKAMKPAAKVYGCEYITRRV
                     KGLTQTNVTTTEDCDAFL"
     gene            complement(1773..2072)
                     /locus_tag="ACHK0Z_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             complement(1773..2072)
                     /locus_tag="ACHK0Z_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4501426.1"
                     /translation="MKLKYPGLTASGKTRTKFMCGDIYRDKYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQAVPRNVPTSREKARANIQEIRKMLNVFRGKK"
     gene            complement(2078..2311)
                     /locus_tag="ACHK0Z_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             complement(2078..2311)
                     /locus_tag="ACHK0Z_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501427.1"
                     /translation="MRDIYHETIYRAFLALSHSENMLEILRIWHETLGDNECDKQKSR
                     IVTALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(2313..2534)
                     /locus_tag="ACHK0Z_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             complement(2313..2534)
                     /locus_tag="ACHK0Z_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501428.1"
                     /translation="MRDDRFNSLKHEFSGVSDDAGDALSSISKLIRASFFLIGTKEYK
                     STGIDVLNIAADYADFVTEVILRKTTDGD"
     gene            complement(2527..2751)
                     /locus_tag="ACHK0Z_RS06890"
                     /old_locus_tag="pgaptmp_001378"
     CDS             complement(2527..2751)
                     /locus_tag="ACHK0Z_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501429.1"
                     /translation="MLQNKTTPDAAAAALTTLMHALIDIECTAVLAQKEEREEYTLFA
                     LECIRYTATRSMNDAKNILIADCENGGGYA"
     gene            complement(2949..3413)
                     /locus_tag="ACHK0Z_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /pseudo
     CDS             complement(2949..3413)
                     /locus_tag="ACHK0Z_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            complement(3406..3603)
                     /locus_tag="ACHK0Z_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             complement(3406..3603)
                     /locus_tag="ACHK0Z_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4501430.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGSHHA"
     gene            3793..4098
                     /locus_tag="ACHK0Z_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             3793..4098
                     /locus_tag="ACHK0Z_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4501431.1"
                     /translation="MATANKNAKSQLTTVRVPLDVMQGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENPLVSSLDALAKVEQIGIKAAEDIGQLVTVAREELQRRKTQESE"
     gene            4223..4552
                     /locus_tag="ACHK0Z_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             4223..4552
                     /locus_tag="ACHK0Z_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501432.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKLRSGLSVQKGGSISGPLFLCLKNSNFVVFQFHQGERIPAHIRA
                     YIQSSWL"
     gene            complement(4602..4790)
                     /locus_tag="ACHK0Z_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     CDS             complement(4602..4790)
                     /locus_tag="ACHK0Z_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4501433.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRMEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(5155..6384)
                     /locus_tag="ACHK0Z_RS06920"
                     /old_locus_tag="pgaptmp_001384"
     CDS             complement(5155..6384)
                     /locus_tag="ACHK0Z_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4501434.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLESYVKENRVDYIALKRRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLKHPQWSL
                     HDIRRTFTTMLNDLGADPHVVEQLTGHQMPGMQRVYNHSRYLDAKRDALDMWTERLGI
                     LAGAHENVTTLPVARRK"
     gene            6372..6554
                     /locus_tag="ACHK0Z_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             6372..6554
                     /locus_tag="ACHK0Z_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501435.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRYTKGYNVKQAGTRKNTNLLIIK
                     NIMHNLKHYETAKNTK"
     gene            6561..7466
                     /gene="tqsA"
                     /locus_tag="ACHK0Z_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /pseudo
     CDS             6561..7466
                     /gene="tqsA"
                     /locus_tag="ACHK0Z_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(7491..8879)
                     /gene="pntB"
                     /locus_tag="ACHK0Z_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             complement(7491..8879)
                     /gene="pntB"
                     /locus_tag="ACHK0Z_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU4501436.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(8890..10422)
                     /gene="pntA"
                     /locus_tag="ACHK0Z_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             complement(8890..10422)
                     /gene="pntA"
                     /locus_tag="ACHK0Z_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU4501437.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKTAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            10946..11890
                     /gene="ydgH"
                     /locus_tag="ACHK0Z_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             10946..11890
                     /gene="ydgH"
                     /locus_tag="ACHK0Z_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU4501438.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            12076..13458
                     /gene="ydgI"
                     /locus_tag="ACHK0Z_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             12076..13458
                     /gene="ydgI"
                     /locus_tag="ACHK0Z_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4501439.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            13495..14217
                     /gene="folM"
                     /locus_tag="ACHK0Z_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             13495..14217
                     /gene="folM"
                     /locus_tag="ACHK0Z_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU4501440.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(14214..14549)
                     /gene="ydgC"
                     /locus_tag="ACHK0Z_RS06960"
                     /old_locus_tag="pgaptmp_001392"
     CDS             complement(14214..14549)
                     /gene="ydgC"
                     /locus_tag="ACHK0Z_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU4501441.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            14678..15397
                     /gene="rstA"
                     /locus_tag="ACHK0Z_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             14678..15397
                     /gene="rstA"
                     /locus_tag="ACHK0Z_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU4501442.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            15401..16706
                     /gene="rstB"
                     /locus_tag="ACHK0Z_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /pseudo
     CDS             15401..16706
                     /gene="rstB"
                     /locus_tag="ACHK0Z_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
     gene            16782..17711
                     /gene="tus"
                     /locus_tag="ACHK0Z_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             16782..17711
                     /gene="tus"
                     /locus_tag="ACHK0Z_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU4501443.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(17708..19111)
                     /gene="fumC"
                     /locus_tag="ACHK0Z_RS06980"
                     /old_locus_tag="pgaptmp_001396"
     CDS             complement(17708..19111)
                     /gene="fumC"
                     /locus_tag="ACHK0Z_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU4501444.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(19254..20900)
                     /gene="fumB"
                     /locus_tag="ACHK0Z_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             complement(19254..20900)
                     /gene="fumB"
                     /locus_tag="ACHK0Z_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4501445.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21099..22274
                     /gene="manA"
                     /locus_tag="ACHK0Z_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             21099..22274
                     /gene="manA"
                     /locus_tag="ACHK0Z_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU4501446.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            22375..23883
                     /gene="ydgA"
                     /locus_tag="ACHK0Z_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             22375..23883
                     /gene="ydgA"
                     /locus_tag="ACHK0Z_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4501447.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLESMEINGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMLALNVPVVPAIPQQ"
     gene            complement(23928..25193)
                     /gene="uidC"
                     /locus_tag="ACHK0Z_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             complement(23928..25193)
                     /gene="uidC"
                     /locus_tag="ACHK0Z_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGU4501448.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLIFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(25232..26605)
                     /gene="uidB"
                     /locus_tag="ACHK0Z_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             complement(25232..26605)
                     /gene="uidB"
                     /locus_tag="ACHK0Z_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU4501449.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSFATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(26602..28413)
                     /gene="uidA"
                     /locus_tag="ACHK0Z_RS07010"
                     /old_locus_tag="pgaptmp_001402"
     CDS             complement(26602..28413)
                     /gene="uidA"
                     /locus_tag="ACHK0Z_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGU4501450.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(28801..29391)
                     /gene="uidR"
                     /locus_tag="ACHK0Z_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     CDS             complement(28801..29391)
                     /gene="uidR"
                     /locus_tag="ACHK0Z_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU4501451.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(29620..30387)
                     /gene="hdhA"
                     /locus_tag="ACHK0Z_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             complement(29620..30387)
                     /gene="hdhA"
                     /locus_tag="ACHK0Z_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU4501452.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(30499..31527)
                     /gene="malI"
                     /locus_tag="ACHK0Z_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             complement(30499..31527)
                     /gene="malI"
                     /locus_tag="ACHK0Z_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU4501453.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            31702..33294
                     /gene="malX"
                     /locus_tag="ACHK0Z_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             31702..33294
                     /gene="malX"
                     /locus_tag="ACHK0Z_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU4501454.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            33304..34476
                     /gene="malY"
                     /locus_tag="ACHK0Z_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             33304..34476
                     /gene="malY"
                     /locus_tag="ACHK0Z_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU4501455.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NMARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            34580..35581
                     /gene="add"
                     /locus_tag="ACHK0Z_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             34580..35581
                     /gene="add"
                     /locus_tag="ACHK0Z_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU4501456.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(35616..36656)
                     /gene="ydgJ"
                     /locus_tag="ACHK0Z_RS07045"
                     /old_locus_tag="pgaptmp_001409"
     CDS             complement(35616..36656)
                     /gene="ydgJ"
                     /locus_tag="ACHK0Z_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4501457.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            36899..37024
                     /gene="blr"
                     /locus_tag="ACHK0Z_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             36899..37024
                     /gene="blr"
                     /locus_tag="ACHK0Z_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU4501458.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         9073 a         9690 c         8924 g         9529 t
ORIGIN      
        1 gtagagggct tttgtctaaa attataaaaa cgcgtcgcaa tcctctgttg ttgtcacatt
       61 ggtttgcgta agtcctttaa ccctgcgcgt tatatattca catccgtaaa cttttgccgc
      121 tggcttcatg gccttgctga aatcagccac gtttagcggc ttgcttcttc ctgcgtatgc
      181 cataaacgcc agatagacgc ggtaaaggct gtttctggtc gtgtacttca cggaatcgcc
      241 accgccaccc atcatcaggc cgcgcgcttc ctccagaaaa ttcaggaact ggcaaaactc
      301 aataaccgga tccgtctgtt gctttattgc cagtgcttca tcaccgtcgc gctgttccag
      361 gagtaaagcc cgtgcctttt cagggtcggc aaagttcgcc agtaatcggc ggataatcac
      421 agggatttca gccgcgacct tctccggcaa atccttgtcc ttttctgcct cgctaacgat
      481 attgtcaaag cgaaaaataa cccgacgacg tgccacgcct ccggcccgtt cggtgaatat
      541 catcgggttg ttgttggtcg tcagcaccac cgccctgatt accgccgtga aacgcttttc
      601 atatttcggg ttaatttcca cggggtcgcc gcccgtgatt ttcttgatgc ccgttccttc
      661 gcctgtatat ttcggctggt ctgccaggac gataagacga ctcccgacaa cctgcgcacg
      721 tccaccagca tcatcaagcg atgtcatttc agcgcttacc gtgttctgtt tccctgccag
      781 aaggctggct atgtgcgtga atgtactttt accgcttccg ccgtctccgg tggcctcaat
      841 aaacatctgc cagtcgtacc ggttcgccat aatcatgtac agcgcggcac atatacgcat
      901 catcttgcgc gggtcttttc cggctgcgtg ctcaagccat ttatgaaaat ttggcgcgtt
      961 atcgcggatg ttctcccctg gtgctggtgg cgtgtactca ataccgttgt gcgtggtgat
     1021 ccagttctcc ggcgtgtgcg gggaaaattc ccccgttttc aggtcaagcg caccattggc
     1081 gaacggcaac aaatcgccgg acggctcgcc cattggttcg gcaataactt ttaacgcttc
     1141 aacggcgtta ttgattacgc gcttgctgaa agtggccctg tgctctgaat agatcgccac
     1201 catttcgcgg ctaagttcca ttgtgctgac cggacaccat aacccgccgc gccatacgtg
     1261 aacgatttca ctttcaggat gtacgcaaac gccatcaaag cgatcggaaa gcagctgagc
     1321 gcgttcactg tccgccatct gcgaaagttg cgccttttgc tttaccggaa gctcaacgaa
     1381 cagaccttca gaaagattct ggcgctcacg ggccagatat tcgcgccagt tctccacctc
     1441 ctgaacgtgc attccctcag ggtaaaaatt tgcatcctgt acacctgccg ccgccagctt
     1501 ctgaccaatc gcctttatca ttacaggttc aagatgtccg gccctgaata tgcgtactga
     1561 ttttcggcct tccggcacaa tttgcagctt atccagttcg gataactgct gatccccaag
     1621 ccacacagga ggctcattat ctccggccat acgcgcgtca tgttcctgcc attgtttagc
     1681 gtgtgaccag gcatcactac ccgcaaaaat aatgacttct gtttctttgt gttttatgcc
     1741 gcgtgactgc tgttttacgt tcggtgcaag tttcattttt tacccctgaa tacgttcaac
     1801 atctttctta tttcctgaat attggcgcgt gctttctccc tgctggtggg cacgttacgc
     1861 ggtacagcct gcaccagaga aaaatcacgg tcgaactgat aaacaggcat aacgcaatca
     1921 tattcgtagc cttcacggcg gtaagttacg cgccgttcct ccacgccctt aatcattacc
     1981 gtgccgccgt acttatcgcg gtaaatatcc ccgcacatga atttagtgcg agtttttccg
     2041 ctggcagtta agccaggata tttaagtttc attattttta ttctccggtg tgctgttctt
     2101 tatatctgtc gtgcaatagg tctatttctt gcagttccat tattacaggc tcaagaagcg
     2161 ttattaatgc cgtaacaatt cttgattttt gtttgtcgca ttcattgtcg ccaagtgttt
     2221 catgccatat gcgcaatatt tccaacatat tttcactgtg agaaagtgca agaaatgcgc
     2281 ggtatattgt ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa
     2341 ataacctctg tcacgaaatc agcataatcg gcggcgatat tcagtacgtc tattccagtt
     2401 gatttatatt ctttcgtgcc aattaagaaa aaagatgctc taatcagttt ggatattgac
     2461 gaaagcgcat cacccgcatc atcagatacg ccggaaaatt catgtttcag ggaattaaaa
     2521 cgatcatcac gcataacccc ccccattttc acaatcagca ataagaatat ttttagcgtc
     2581 attcatcgac cgcgtggcgg tgtatcggat acattccagg gcgaacaatg tgtattcttc
     2641 ccgttcttcc ttttgtgcaa gcactgctgt gcattcaata tcaataagtg cgtgcatcag
     2701 cgtagtgagc gcggcggctg ctgcgtccgg tgtggtttta ttctgcaaca tatcttaact
     2761 ccactgaact tacgtattta cacgggtttc cttttacctg tcgcacacgg taggaatcgt
     2821 ttttaatcac ttccgctatt tgcccaaact tttttaccgc tgtacggcgg ctcttgtagc
     2881 ggtaagtgtt tatctccctt ctgctcatta ccgcttcatt gtgatatctg gttatcacta
     2941 tcagcacttc aaacatgacg caccccctga cgaatacggg cgacaaaaat cagatgtgcg
     3001 cgcggcaact gggcgcgggc ttcgcgttca gtggctgcgg taacggtgta aatatcaatg
     3061 ctgctgtggt ggcactgcat aaagcgccag acaaagtaag ggcgtgcgga tacagccatg
     3121 tgaaaggctc ccaatttaat gttaaggggt ctcaccacta cgctgctaaa cggggtggcg
     3181 agacgtaaca gggttagcag actggcaaat tgggaaacca gcgagcgcaa aggctccccc
     3241 gttacgcccc gccataatgc gggtatgggt aggtttacgg acacaaaaaa accgcttatc
     3301 ggatatgggc ggctgtccgc caatttattc aggctgctaa acccggtcgc catgtgggcg
     3361 acgggggaag catacagccc cgtgataatt ttttgcaagc ggtttttacg catgatgtga
     3421 cccctgacga atacgagcgg caaatacagc aacacaacct gacgggcaac ggctacgcgc
     3481 ttcgcgttcc gtccaggcgg ttacgtggat gatttgagat tctccggcac tcagggccag
     3541 aaaacgccac acaaaggccg tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac
     3601 cataacggct cctagtttac gttgttggtt agaagccccg ttactgctcc tacagtgcgg
     3661 ggctttgctc tttgtatttc aacaatcctt ttggtgtgtt tcatgttatg gacacatgaa
     3721 acacacgtca aggctttttg tatttctttt tttgtgtata ctgaaacaca ccgatgatta
     3781 ggagtttcag aaatggcaac ggctaacaaa aacgcaaaat cacaactgac aaccgtcaga
     3841 gtcccactag atgttatgca agggatggaa tccgttaagc tggacggcga aagcaatgcc
     3901 ggatttatcg taaccgccat gcgcggtgaa atcgcccgcc gccaggcaga aggaagcgga
     3961 gaaaatcccc ttgtgtcttc actggatgct ttggctaagg tcgaacaaat tggcatcaag
     4021 gcagcggagg atatcgggca gcttgtcacc gttgcacgcg aagaactcca gcgtcgcaag
     4081 acccaagaat cagaataatc actatcagcg ccgtggcgtg aggtactacg gcgcattgct
     4141 ttacaggcgc acacaatgac caacgaagaa tcaaccgaaa aaacatcacc tgcacgaaaa
     4201 agacgacgca gaaagaaaga gcatgaatca gaacgattcg cgccgtgttc ttttgccctt
     4261 gagaagtacc ttaaagaata ctcatcaaag gagagagctt cgcagttatg gcaacgcact
     4321 gaaccagact gattgcattg cacaccagcc tgatagcggc tatcatcccc gtgcttatgt
     4381 ttgggatcac acacacaaac tgcgcagcgg gttatctgtt cagaaaggcg gctccatttc
     4441 ggggccgctt tttttatgcc tgaaaaactc caattttgtt gtttttcagt ttcaccaggg
     4501 cgaacgaatc cctgcccaca ttcgggcgta tattcaatct tcatggttat agctctgtgt
     4561 tcagatgatt gatgtgtggc ggctgcgtgc cgccagcgtg attaatgaac tgccttgcag
     4621 ctatccttcc aggccagaac ctcggataaa gaccagccaa cggaacgacc gccaagttta
     4681 cgacgtgatg ggaattgtcc ggccttttcc atgcggtagc gacacgtacg gctaaggccg
     4741 gttagttttt cgcattcttt ttcacgtata aaccgatcag tgcttaacac tattgccccc
     4801 tttcgtttct taaagagtca tcaggtgtct tattgtgtcg tattgttccc gctagagtga
     4861 tgaatggcaa atgttggtgg cgcatggttt acagaaagag gaataatcag gataaaatca
     4921 tttaaattca tgttaataca aaggcataaa atctcgtttt atgccttttt tctcacgctt
     4981 taaagcgtga ttcgctaatg tataaaaaac cagtaatata ataaaaatca gcaacttata
     5041 aatctgtatg ctttttcgcc tcttgttcgt gattgttcca cgttgttgca cattgtttca
     5101 cgttgtatct gttcgcatat ccagtatgcg catactgaaa aaacccgaaa aaaattattt
     5161 tcttctggct acgggtagcg tggttacgtt ttcatgtgct cccgccagta tccctaaccg
     5221 ctccgtccac atatccagcg catcacgttt cgcatccaga taacgggaat ggttatagac
     5281 tcgttgcatc cctggcatct gatgacctgt aagctgctcc acgacgtgag gatccgcgcc
     5341 taaatcgttc agcatcgttg taaaggtgcg ccggatgtca tgcagtgacc attgagggtg
     5401 ttttagcctc ctgtgcgcta atctgccgta ctgcgatacg ctggcctcct gtttcacttc
     5461 ccccagcaat aagcccgtgt gcctgttctg ctccaccagc ttcatgacaa acggcaggat
     5521 cgcttccggt atgggccgga atattgcgac cttcgttttg ctgtgctcct tcggaacggt
     5581 ccatagcatt tccgtaaaat cccactcccc gatctccgat aacctcagtt ctaccgttct
     5641 ggcaccgaag acaatcagga ggcggattaa cgcgacgtag taaggggaaa atattttttt
     5701 atccagtgcc tgcaataact cgccaagttc tttggtgctt aagacacgct cgcttatatc
     5761 cggttttttc ccaacgtccg caacgttcag atcgtccaga acgttgctga ttgcatagcg
     5821 ccgcctacgg cagaacttaa gcgcctgttt gcacgcctgt agcacgaatc cggcagtaac
     5881 aggcgttcgc tttgccacct ggtcaaaaca agccagccag tgccgtagct cgcatttatc
     5941 cagcggcata gcaccaatgt gctgtattac gtgattatta agtcgccttt tcagggcgat
     6001 ataatccacg cggttttcct ttacgtaaga ctcaagccag taggtaagcg catcgccaac
     6061 cgttacgggc tttaacgctt cctgtacggt gtaattcatc tcatgacgtg gatttttccc
     6121 ctcagccagc catgcgcgac actgtgcggc tttttccctg gctgctttca ggctcagatc
     6181 aggataactt cccagcttaa tgcgttcggg tcgtgtctcc cttcccgttc cggccctgta
     6241 tgtgaaatac catgttaatt tccctgatgt taaatatttc acgctcaggt ttccgccatc
     6301 actataaaac gtgtttttct cagctggctt accatgaagt ttccttagca atgtatcgct
     6361 cagtttgttc attgctctgc tcacgtttta ccccctcttt acgaaaaagt gactacacca
     6421 gtgactacac cgatcgttac acaaagggct acaacgtgaa acaagctgga acaaggaaaa
     6481 acacaaatct gttgataatc aaaaacataa tgcacaacct gaaacattat gaaacagcaa
     6541 aaaacactaa atgataaaat gctgttattc ttaacccgct ggtgcaacac atggtccgct
     6601 ggcgagtgcc gcgtgtactg gcggtgtcga ttttgatgac catcatcgtg atggcgatgg
     6661 tgttgctatt agcttatctg ggttccgcgc tcaacgagtt gacgcggacg ttaccgcaat
     6721 atcgcaactc tattatgacg ccgctgcaag ctcttgagcc gttgttgcaa cgcgtaggga
     6781 ttgacgtctc agttgaccag ctggcgcatt acattgatcc gaacgcggcg atgacgttgc
     6841 tcaccaactt attgacgcag ttatctaatg ccatgtcatc aatattttta ttgctgctga
     6901 cggtgctgtt tatgctgctc gaagtgccac aattgcccgg aaaatttcag caaatgatgg
     6961 cgcgtccggt tgaagggatg gcggcgattc aacgtgcaat tgacagtgtt tctcattatc
     7021 tggtgctgaa aacagccatc agcatcatca ccggcctggt cgcctgggcg atgctcgccg
     7081 cactcgatgt tcgcttcgct tttgtctggg gattgatggc ctttgcgttt aattacatcc
     7141 ctaatattgg ttcagttctc gcggcaatcc cccctatcgc tcaggtactg gtgtttaatg
     7201 gcttctacga agcgttgctg gtgctggcgg gatatctgct gattaatctg gtcttcggca
     7261 atattctgga gccgcgcatc atgggacgtg ggctggggct ttccacattg gtggtatttt
     7321 tgtcgttgat tttttgggga tggttgttag gaccggtggg tatgctgctt tccgtgccgt
     7381 taacaattat tgtcaaaatt gcgcttgaac aaacagcggg aggtcaaagc atcgccgttc
     7441 tgttaagcga tctcaataaa gagtgacggc ctccgcagag gccgtcaggg ttacagagct
     7501 ttcaggattg catccacgct ggctttggcg tcaccaaaca gcatgtgggt gttttccttg
     7561 aagaacagcg ggttttgcac accagcatag ccagtgttca tcgaacgttt aaagacaatc
     7621 acgttctgcg ctttccacac ttccagcaca ggcataccag caatcggact cttcggatca
     7681 tcctgcgccg ccgggttaac cgtatcgtta gcaccaatca ccagtacggt atcggtatca
     7741 gcaaagtcat cgttgatctc gtccatttcc agcacgatgt catacggtac ttttgcttca
     7801 gccagcaata cgttcatatg tccaggcaaa cgccccgcaa ccgggtggat accgaaacgc
     7861 acgttgatac cacgagcgcg gagtttctcg gtaatttcag cgacaggata ttgcgcctgc
     7921 gcgactgcca tgccgtaccc cggagtaatg atcactgaat gggagttttt cagcagttcc
     7981 gctgtctctt ctgcggtgat ttcgcggtgc tcacccactt cctgatcatc gccagtagaa
     8041 gagccgtcgg tgccgaaacc acccgcaata acgctgataa aggaacggtt catcgcctta
     8101 cacataatgt aagaaaggat agcccccgaa gaaccgacca gcgcaccggt cacaatcagc
     8161 aggtcgttgc tgagcataaa gcccgcagcc gcagccgccc agccggagta cgagttcagc
     8221 atcgacacca ccactggcat atctgcacca ccgatggagg cgactaaatg ccagccgaat
     8281 accagcgcaa tggcggtcat tatcagcaat gccagcactt gcaggccgac gctgtccgtg
     8341 cgaacaaata caatcagcag caggaaggaa acgaccagag ccgccaggtt cattttgtga
     8401 cggtttggca gcatcaacgg tttagacgaa atcttgccac acagtttgcc gaacgccacc
     8461 accgaacccg tgaacgttac cgccccgatg aagataccga ggaacacttc cgtcaggtga
     8521 atattgacca gaatcggtgc cattcccgcg tcatgatgca gataactgtt aaagccaacc
     8581 agcactgccg ccagacccac gaagctatgc aggatcgcca ccagttctgg catttcggtc
     8641 atttcaactt tcttcgccag acggatacca attgccccac caatgaccat cgccagcaag
     8701 atccagccaa cattacccgt atccggtcca aaaatggttg cgattaacgc aatcgccatc
     8761 ccggcgatac cgaagttgtt accctggcga gacgtttcat gtttcgaaag accggccaga
     8821 ctgaagataa acaggatcgc ggcaacaatg tatgcagctg taactaatcc tccagacata
     8881 tgttacccct taatttttgc ggaacatttt cagcatgcgc tgagtcacgg tgaagccacc
     8941 gaaaatatta atgctggcta taagcaccgc gataaaacta aggaagctaa cccagccgcc
     9001 ctggccaatc tgcaacagtg ctccgacaac aataatccct gaaatcgcgt tggtgaccga
     9061 catcaacggt gtatgcagcg catgcgatac attccacacc acgtaataac cgacaacgca
     9121 ggccagcgcg aaaacggtga agtgcccaag aaactctttc ggcgcaacgc ttgccagcca
     9181 accgaaaagg atgattgcca gcgccatcaa tgcgtattta cgccacggtg agcaggcaca
     9241 tttttcctca gttttcactt ccggtgccgc tttttgtgcc gcctgcggct gagctgatac
     9301 ctgaatcggc ggtgccggcc aggtaatttc gcccgcacgg atcacggtca cgccgcgaat
     9361 caccacatca tcaaaatcaa cagtgatatt gccgtctttc tctttgcaca acagtttcag
     9421 cagattaacg aggttagtac cgtaaagctg tgaggattgc gtcggcagac ggcccggaag
     9481 atcggtataa ccaatcactt tgacaccatt ttccgtagtg aagatttcac ccggcacggt
     9541 gtattcacag ttgccaccgt tttgggctgc caggtcgaca atcacactgc ccgccttcat
     9601 ggagtcaacc atttcacggg taattagctt cggcgctgtt ttgcctggaa taagcgcggt
     9661 ggtgacaatg atatcgacct cttttgcctg ggcggcaaag agttccattt ccgctttgat
     9721 gaacgcgtcc gacatcactt tggcatagcc atcgccgctg cccgcttcct ctttaaaatc
     9781 cagctcgagg aattccgcgc ccatactttg aacttgttct ttcacttccg ggcgggtgtc
     9841 gaatgcacgc acaatcgcgc cgagactgtt tgctgcgcca atggcggcca gacctgcaac
     9901 acccgcacca atcaccatca cttttgccgg tggcactttc ccggccgcag taatttgccc
     9961 ggtaaagaag cgcccaaatt catgtgccgc ttcaacaatg gcgcgataac cggcgatgtt
    10021 cgccatcgag cttagtgcgt ccagcgattg tgcgcgtgag atacgcggca cagaatccat
    10081 cgccatcacg gtcacgttac gttccgcaag tttttgcatt aattccggat tctgcgcagg
    10141 ccagataaaa ctcaccagcg ttgtcccagg attcagtaac gcaatttcat catctaacgg
    10201 cgcattgacc ttcagaatga tctctgactg ccagacgcta ttcccttcta caatttcagc
    10261 gcccgcttgc acaaacgctt tatcgtcaaa acttgccagt tgacccgcgc cgctctctac
    10321 cgcgacggta aaacccagtt tcagcagctg ttccactgtt tttggcgttg ctgcaacacg
    10381 ggtttcattg gttaaccgtt ctcttggtat gccaattcgc atgatattcc cttccatcgg
    10441 ttttattgat gatggtttgc ctgtgtcagg agccacacaa gccgctcatg tacgagctaa
    10501 atgttactcc gttaaaataa attagtaaca aacgccctat aacgtactga aaattatgcc
    10561 tgtgatctag cgccaaaaat cagtatttcg gcgtgaactc gcaaaatatt aacgattcag
    10621 ccgtgatagt gggataaaca ccttagaacg ccggataaag actgataatt gtcttcgacg
    10681 gtcgggtaaa acgagacatc gccccggcac gaatcactac ttaacattaa attaacttat
    10741 ataattcagt tgcttcagta gtaatgatgc tgatacggct gttttttaag catagacggt
    10801 catttgagca ggattaaaat tggcttaaag aatgtgatat gaaaaatgac gcagacagtt
    10861 acaccgttta aatgcaataa tcagccacgt ttctcgttaa taacaatacc agtacctggt
    10921 ttgcgcaagg cgaaggatta tttttatgaa gcttaagaac accctcctgg cgtcggcact
    10981 gctttctgct acggcattct ccgttaacgc agcaacagaa ctgacaccgg agcaagcggc
    11041 agcggttaaa ccttttgacc gtgtagtggt taccggtcgt tttaatgcta ttggcgaagc
    11101 ggtgaaagcc gtttctcgtc gcgcagataa agaaggtgcc gcctcttttt atgttgtcga
    11161 cacttctgat tttggtaaca gcggtaactg gcgtgtggtc gctgacctct ataaagccga
    11221 tgctgaaaaa gcagaagaaa caagtaatcg cgtaattaac ggtgttgtcg aactgccgaa
    11281 agatcaggct gttctgattg aaccgtttga cacggtcacc gtccagggct tctatcgtag
    11341 ccagccagaa gtcaatgatg ccatcaccaa agcggcaaaa gcgaaaggtg cctactcttt
    11401 ctacatcgtt cgtcaaatcg atgccaacca gggcggcaac cagcgtatta ctgcattcat
    11461 ctataaaaaa gatgctaaga aacgtatcgt ccagagcccg gatgtgatcc cggcagattc
    11521 cgaagcagga cgtgcagctc tggctgccgg tggcgaagcc gcgaagaaag ttgagatccc
    11581 aggtgttgcg actaccgcat caccaagttc tgaagtcggt cgtttctttg aaacccagtc
    11641 atcaaaaggc gggcgttaca ccgtcacgct cccggatggc actaaagtcg aagaactgaa
    11701 caaagcgacc gcagcgatga tggtcccgtt cgacagcatc aaattctctg gcaactacgg
    11761 caacatgacc gaagtctcct atcaggttgc gaaacgtgcc gcgaagaaag gtgctaagta
    11821 ttaccacatc acccgccagt ggcaggaacg tggtaataac ctgaccgtca gcgcagatct
    11881 gtataaataa ctattcagtt actcctataa ggcggcttga tgccgccttt cgcattttta
    11941 gtcacaaatt tccccgcgac cattgcatgc agacacatct cttcgtaaaa tcccgcgctt
    12001 cagtggcaaa ctccattttt atgcgttttg ccctaatatt tattcttctt atcacgtttt
    12061 aatcactgga tatcgatgga aaagaaactg ggactgagcg cactcaccgc gctggtatta
    12121 agctcaatgc tgggcgcggg tgttttcagt ctgccgcaaa atatggcggc agttgccagc
    12181 ccggcagcac tgctcatcgg ctggggtatt actggcgctg gcattttatt gctggccttt
    12241 gccatgctga tcctcacgcg cattcgtccc gaacttgacg gcggtatctt tacctatgcc
    12301 cgcgaagggt ttggcgagct aatcggcttt tgttccgcat ggggatactg gctgtgcgca
    12361 gtcatcgcca acgtctccta tctggtgatc gttttttccg cgttaagctt ttttacggac
    12421 acgccggaat tgcgcctgtt cggcgatggt aatacctggc aatcgatagt cggtgcatcg
    12481 gctttattgt ggattgttca ctttttgatc ctgcgcgggg tgcaaaccgc tgccagcatt
    12541 aacctggtgg cgacactggc aaaattgttg ccgctgggtc tgtttgttgt gctggcgatg
    12601 atgatgttca aactggatac cttcaagctc gacttcaccg gacttgcact tggcgtaccc
    12661 gtgtgggaac aggtgaaaaa caccatgctg atcaccctgt gggttttcat tggtgtggaa
    12721 ggtgccgtgg ttgtttctgc gcgtgcgcgt aataaacgtg atgttggcaa agcgacactg
    12781 ctggcggttc tctccgctct gggcgtttac ttgctggtaa cgctgctttc gctgggcgtg
    12841 gtggcacgtc ctgaactggc tgaaattcgt aacccgtcaa tggccggtct gatggtggaa
    12901 atgatgggac catggggcga aatcatcatc gctgccggtt tgatcgtttc cgtttgcggc
    12961 gcgtacttaa gctggaccat catggcggca gaagttccgt tcctggctgc cactcataaa
    13021 gcattcccac gcatttttgc gcgccagaac gcacaagctg cgccatccgc ctcgctgtgg
    13081 ttgaccaaca tctgcgtgca aatttgtctg gtactcatct ggctgaccgg ttccgattac
    13141 aacacgttgc tgaccatcgc ctcagaaatg attctggtgc cctatttcct ggtcggcgcg
    13201 ttcttgctga aaatcgcaac ccgtccactg cataaagcgg taggtgtcgg tgcctgcatt
    13261 tatggcttat ggttattgta cgcctctggt ccaatgcact tgctgttatc cgttgttctg
    13321 tatgcgccgg ggctgctggt tttcctgtat gcgcgcaaaa cgcatacaca tgataatgta
    13381 ctgaaccgtc aggaaatggt gctgattggc atgctgttaa ttgcctcagt tccggcgacc
    13441 tggatgctgg tgggataacg ttcatcccat cgttggatag caaaggagat aacgatgggt
    13501 aaagcccagc ccttgccaat attaattact ggcggaggtc gtcgcatcgg cctcgccctc
    13561 gcctggcatt tcattaatca aaagcaaccg gtgattgtca gctatcggac gcactatcca
    13621 gccattgatg gactgattaa ggcaggtgcg cagtgtattc aggctgattt ttcgaccaac
    13681 gacggtgtga tggcgtttgc cgatgaagta ctaaaaagca cccatggtct gcgtgctatt
    13741 ttgcataacg ccagtgcgtg gatggcggaa aaaccaggta caccactggc cgacgtactg
    13801 gcttgcatga tgcagatcca cgttaatacc ccatacctgc tcaaccatgc gctggaaaga
    13861 ttactgcgtg ggcacggaca cgccgccagc gatatcattc actttaccga ttatgtggtg
    13921 gagcgcggta gcgacaaaca tgttgcgtat gctgcaagca aagcggcact ggataatatg
    13981 acccgctcgt ttgcccgcaa gctggcaccg gaagtgaaag tgaattctat tgcgccatcg
    14041 ctgatcctgt ttaatgaaca tgatgatgcc gaatatcgac aacaggcgct gaataaatca
    14101 ctgatgaaaa ccgcgcctgg cgagaaagaa gtgatcgacc tggtcgatta cttacttacc
    14161 agctgctttg tcaccggacg cagtttccca cttgatggcg gtcgtcatct gcgttaatgc
    14221 agctttatcc agcatataat caacacccat gcgcttatcc cccaacacgc aacagaacca
    14281 acaaaagcgg cgggcaagcg catcattccg gtgaaatacc acagcgacac caggtaaaca
    14341 aaatagggaa taatcgacca catactaaaa atgatggttg cgcgtaaggc ttcaatgccg
    14401 cgttcgctgg caacaatata atgcgcgata agcgcaaagg tcgggaaaag tggaatcagc
    14461 ccggcgatat aataattttt cgtttttgct aaaacaccaa tcaacagcac taccagcgca
    14521 ccgagcgcgg ctttgattac cagccccatc tttttacctt aacacttcca taacaagtca
    14581 tcagtagaat acctgatgaa aacttgttta gaaacgattg atagtaagta aaaacagcgc
    14641 ggtgtattgt gacgttttta tatctaccgt gaatgttatg aacactatcg tatttgtgga
    14701 agatgatgcg gaagtcggtt cactgattgc cgcgtacctg gcaaaacatg atatgcaggt
    14761 taccgtagaa ccgcgcggcg accaggccga agaaaccatt ttgcgagaaa gtccggattt
    14821 ggtgttactc gacatcatgc taccaggcaa ggacggcatg accatttgtc gtgatttacg
    14881 cgcaaagtgg tctggaccga ttgttcttct aacctctctc gatagcgata tgaaccacat
    14941 cctggcactg gaaatgggtg cctgcgacta tattctcaaa acgacgcccc ctgctgtttt
    15001 gctagcgcgt ttacgtttgc atttgcgtca gaatgagcaa gccacactga ccaaaggtct
    15061 tcaggaaacg tctctgactc cctacaaagc cctgcatttc ggcacgttga ccatcgatcc
    15121 catcaaccgc gtagtcaccc tggctaacac tgaaatctcg ctctcgacag ctgatttcga
    15181 attattgtgg gaattagcta cccatgccgg gcaaatcatg gaccgcgatg cattgctgaa
    15241 aaatttacgc ggcgtcagtt atgacggact ggatcgtagc gtggacgtgg ctatttcgcg
    15301 gttaagaaaa aaactgctcg ataacgccgc agaaccttat cgcattaaaa ctgtgcgtaa
    15361 caaaggctat ctttttgcgc ctcatgcatg ggaataagcg atgaaaaaac tgtttatcca
    15421 gttttacctg ttattgtttg tctgcttcct tgtgatgtct ctgctggttg ggctggtgta
    15481 caaatttacc gccgaacgcg cgggcaaaca gtcgctggat gatttgatga acagttcgct
    15541 gtatctgatg cgcagcgaat tgcgagagat cgatcccccc acacgactgg ggtaagacgc
    15601 tgaaagagat ggatttaaat ctctctttcg atctgcgtgt cgagccactg agtaaatacc
    15661 atcttgatga tatttccatg caccgactgc gtggcggcga aattgtcgcc ctggacgatc
    15721 agtacacgtt tttgcagcgt atcccgcgca gccactacgt gctggcagtt ggtcctgttc
    15781 cttatcttta ttatctccat cagatgcgat tgctggatat cgccctgatc acttttattg
    15841 ctatttccct cgcctttccg gtgtttatct ggatgcgtcc gcactggcag gatatgttaa
    15901 aactggaagc agcggcgcaa cgatttggcg atgggcatct caatgaacgt atccactttg
    15961 atgagggttc gagctttgaa cgacttggta tcgcatttaa ccagatggcg gacaatatca
    16021 acgccttgat tgccagcaaa aaacagctta ttgacggtat cgctcacgaa ctgcgaacac
    16081 cgttagtgcg cctgcgttat cgactggaga tgagcgataa cctgagcgcc gccgaatccc
    16141 aggcgttgaa tcgtgatatc agtcaacttg aagctttaat tgaagagctg ctgacttatg
    16201 cccgactcga tcgcccacaa aacgagcttc atcttagcga accagacctg ccgttgtggc
    16261 tgtcaacgca tctggcagac attcaggcag taacgcccga taaaacggta cggattaaaa
    16321 cgctcgcgca aggccattat gcggcgttgg atatgcgctt aatggagcgc gtgctggata
    16381 atttgctcaa taacgccctg cggtactgcc attcaacggt tgaaaccagc ctgctactgt
    16441 cggggaatag agcgacatta attgttgagg atgatggccc agggattgcc ccagaaaacc
    16501 gcgaacatat ctttgaacct tttgttcgcc tcgatcccag ccgggatcgc tcaaccggcg
    16561 gctgcgggct ggggctggca attgtccact ctatagcact ggcaatgggc ggtacggtta
    16621 attgtgacac cagcgaactg ggtggtgctc gcttctcgtt tagctggccg ttatggcata
    16681 acatcccgca atttacctct gcctgacact acgcgcacga tggtcaagtc accaccactg
    16741 tgctataaaa taagtatgtt gtaactaaag tggttaatat tatggcgcgt tacgatctcg
    16801 tagaccgact caacactacc tttcgccaga tggaacaaga gctggctgca tttgccgctc
    16861 atcttgagca acacaagcta ttggttgccc gcgtgttctc tttgccggag gtaaaaaaag
    16921 aggatgagca taatccgctt aatcgtattg aggtaaaaca acatctcggc aacgacgcgc
    16981 agtcgctggc gttgcgtcat ttccgccatt tatttattca acaacagtcc gaaaatcgca
    17041 gcagcaaggc cgctgtccgt ctgcctggcg tgttgtgtta ccaggtcgat aacctttcgc
    17101 aagcagcgtt ggtcagtcat attcagcaca tcaataaact caagaccacg ttcgagcata
    17161 tcgtcacggt tgaatcagaa ctccccaccg cggcacgttt tgaatgggtg catcgtcatt
    17221 tgccggggct gatcaccctt aatgcttacc gcacgctcac cgttctgcac gaccccgcca
    17281 ctttacgctt tggttgggct aataaacata tcattaagaa tttgcatcgt gatgaagtcc
    17341 tggcacagct ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg acgcgcgagg
    17401 agtggcaaag aaaactggag cgagagtatc aggatatcgc tgccctgcca cagaacgcga
    17461 agttaaaaat caaacgtccg gtgaaggtgc agccgattgc ccgcgtctgg tacaaaggag
    17521 atcaaaaaca agtgcaacac gcctgcccta caccgctgat tgcactgatt aatcgggata
    17581 atggtgcggg cgtgccagac gttggtgagt tgttaaatta cgatgccgac aatgtgcagc
    17641 accgttataa accacaggcg cagccgcttc gtttgatcat tccacggctg cacctgtatg
    17701 ttgcagatta acgcccggct ttcatactgc cgaccatctg ttctggccgt acccagctgt
    17761 caaactcggc ttcgctaaga taccccagcg caagggccgc agcttttaag gtcagccctt
    17821 ctttatgcgc ttttttggcg atctccgcgg ctttgtcata accaatgtgg gtgttaagcg
    17881 cagtcaccag catcagcgat tcattgagta attgattgat tcgctcacga ttcggttcaa
    17941 tacccactgc gcagtgtttg ttaaaacttt ccatgccatc tgccagcaag cgcaccgatt
    18001 gcaggaaatt atggatcacc atcggacgga agacgttcag ttcaaagtta ccggaagcgc
    18061 cacccatgtt gatcgccacg tcgttcccca ttacctgaca gcagagcatg gttaatgctt
    18121 cacactgggt tgggttcact ttccccggca tgattgagct gcccggctca ttttccggga
    18181 ttgagatttc accaattccg cagcgcgggc cagaggccag ccagcggaca tcattggcga
    18241 ttttcatcag tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc agggcatcac
    18301 aggtcgcaag cgcttcaaat ttgttcggcg cggtaacaaa cggtgcacag gtaatgactg
    18361 ccagttcatc tgctacgcga cgcgcatact ccggatgggt atttagtcca gtacccaccg
    18421 ctgtaccgcc aagagccagt tccgctacgt gaggcaggct gtattcgata tgtttgagat
    18481 tatgctcgag catcgctacc cagccggaaa tctcctgccc tagtgttagc ggcgtggcgt
    18541 cctgcaagtg ggttcgaccg attttgacga tatcggcaaa tgcacgcgat ttttcactca
    18601 gtgtctgtgt cagggtttta agctgcggaa tgagttgctt gcgcagcgcc agcagcgccg
    18661 caacgtgcat cgccgtcgga aagacatcgt tggaactttg gcttttgttc acgtcgtcgt
    18721 tagggtgaac tttacgttcc atcccgcgca cgccgccgag taattcactg gcccggttag
    18781 ccagcacttc gttcatgttc atgttacttt gcgtgccgga gccggtctgc cagatagcca
    18841 gcgggaattc gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc cgaatggcgc
    18901 tcgctttctc ttcagacaac aagcctaaat cttcattaac ttttgccgct gcgcgcttgg
    18961 ttagcgccag cgcatgaatc agtgaggtgg gcattttctc cgtcgaaatg cggaaatgct
    19021 ccagcgagcg ttgagtttgt gcgccccaca gcttatctgc cgggacatca atcgccccca
    19081 tcgaatcttt ttcgctgcgt actgtattca tggcctgctc ctcacctgtt taattttttc
    19141 tttctgtttt gctttcgtta agcaactttt agctcactta ttatttacta tttgataaca
    19201 aatgtttggt ctttcgtgcc atgtaaaaaa accgccccga agggcggctc tgtttatttc
    19261 acacagcgag tgcactgtgt gagttgtatc tgctggaaga agtcatttcc tttatcatcc
    19321 acaaggataa acgccgggaa atcttccact tcaattttcc agatggcttc cattcccagt
    19381 tccggatatt caacacattc caggctctta atacttccct gcgccaatac agcggccgga
    19441 ccaccgatac tgccaaggta gaagccgccg tgttttttac aggcatccgt cacctgctgg
    19501 ctgcggttgc ctttcgccag catgatcata cttccgccct gcgcttgcag ttgatcgaca
    19561 taagaatcca tccgtccggc ggtcgttggg ccaagagaac cggaggcata accttccggc
    19621 gttttggccg gacccgcgta gtaaatcgga tgatctttaa tgtactgcgg cagcccttca
    19681 ccgttatcca tccgctcttt cagtttggcg tgagcaatat cacgaccgac gataatcgtg
    19741 ccgttaagcg ataagcgtgt agaaacggga tactgcgaca actgtgcgag gatctctttc
    19801 atcggacggt taaggtcaac gcgcaccgct tcgccttctc ccgctttacg cagctcttca
    19861 gggatatatt tacctggatt atgttccagt ttttcgatcc agatcccctg acggttgatc
    19921 ttcgctttga tattacggtc agcagagcag gaaacaccca taccaaccgg acaggatgcg
    19981 ccgtgacgtg gcaggcgaat cacgcgtatg tcgtgagcga agtatttacc accaaactgc
    20041 gcacccagac cgagattttg cgcttcgatc agcaattctt tttccagttc cacatcgcgg
    20101 aatgcctgac cgtgctcatt cccttccgtt ggcagttcgt catagtattt agcggaagcc
    20161 agtttcactg ttttaaggtt agtttctgca gaagttccac caattacgaa cgcaatatga
    20221 tacggaggac aggccgccgt acccagcgtg cgcatcttct caaccaggta atttttcagt
    20281 ttccccggcg ttagtaacgc tttggtttcc tgatagagat acgtcttgtt tgccgaacca
    20341 ccacctttgg cgatacagag gaatttgtac tcgtcgccat caacggcata aagatcgatc
    20401 tgcgctggca gattggtgcc ggtattcacc tctttataca tatccagcgg cgcgttttgc
    20461 gagtagcgca aattatcttc gatataagtg ttatagacac cgcgcgccag cgccgcttca
    20521 tcacagccac cggtccatac acgctgccct tttttaccaa caataatcgc agtgccggta
    20581 tcctgacagg ttggcagaac acctttcgcc gcgatgtcgg agttgcgcag gaattgcagc
    20641 gccacatatt tatcattttc gctggcttcc gggtcacgca gaatgtcggc cacctgttgt
    20701 tggtgagccg gacgcagcat aaatgacgca tcatgaaatg cctgacgcgc caacagagtt
    20761 aacgcttcgg gtgcgacttt caaaatctcc tgcccttcaa attcagatac gctaacgtgt
    20821 tcgctggtta gcaggtaata ctcagtatca tcttttttga gtggaaaagg agcctgataa
    20881 tgaaagggtt tgtttgacat tgttctctca cttactgcct ggtttggtta tgctctgggc
    20941 gggtgttccg ttgccctgtt aaaagcgagt aacaatatcc tacacacttt tttaacaaaa
    21001 actgagacta gtacgacttt ttgcggctcc aggttacttc ccgtaggatt cttgctttaa
    21061 tagcgggatt aatttccaca ttaaaacagg gattgatcat gcaaaaactc attaactcag
    21121 tgcaaaacta tgcctggggc agcaaaacgg cgttgactga actttatggt atggaaaatc
    21181 cgtccagcca gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc agttcacggg
    21241 tgcagaatgc cgccggagat atcgtttcac tgcgtgatgt gattgagagt aataaatcga
    21301 ctctgctcgg agaggccgtt gccaaacgct ttggcgaact gcctttcctg ttcaaagtat
    21361 tatgcgcagc acagccgctc tccattcagg ttcatccaaa caaacgcaat tctgaaatcg
    21421 gttttgccaa agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt aactataaag
    21481 atcctaacca caagccggag ctggtttttg cgctgacgcc tttccttgcg atgaacgcgt
    21541 ttcgtgagtt ttccgagatt gtctccctac tccagccagt cgcaggtgca catccggcaa
    21601 ttgctcactt tttacaacag cctaatgccg aacgtttaag cgaactgttc gccagcctgt
    21661 tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg gcgctagaca
    21721 gtcagcaggg tgaaccgtgg caaacgattc gtttaatttc tgaattttac ccggaagaca
    21781 gcggcctgtt ctccccgctg ttactgaatg tggtgaaatt gaatcctggc gaagcgatgt
    21841 tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcga
    21901 actccgataa cgtgctgcgt gcgggtctga cgcctaagta cattgatatt ccggaactgg
    21961 ttgccaatgt gaaattcgaa gccaaaccgg ctaaccagtt gttgacccag ccggtgaaac
    22021 aaggtgcaga actggacttc ccgattccag tggatgattt tgccttctcg ctgcatgacc
    22081 ttagtgataa agaaaccacc attagccagc agagtgccgc cattttgttc tgcgtcgaag
    22141 gcgatgcaac gttgtggaaa ggttctcagc agttacagct taaaccgggt gaatcagcgt
    22201 ttattgccgc caacgaatca ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt
    22261 acaacaagct gtaagagctt actgaaaaaa ttaacatctc ttgctaagct tattaaaggc
    22321 ttataacacc ttcaggcggc cagtccgcct gatttcattt tatggataat cattatgaat
    22381 aaatcgctgg tagcggtagg cgtcattgtt gcgctaggcg tagtctggac aggcggcgca
    22441 tggtatacag gcaagaagat tgaaacccat ctcgaagaca tggtcgcgca ggcgaacgcg
    22501 caactcaaac tgacagctcc tgaatccaac ctggaagtga gttatcaaaa ctatcatcgc
    22561 ggcgtattca gcagccagtt gcaactgttg gtgaaaccca ttgccgggaa agaaaatccg
    22621 tggattaaaa gcggtcagag cgtcatcttc aacgaatcgg ttgatcatgg tcccttcccg
    22681 cttgcccagc ttaaaaaact gaacctgatc ccgtcgatgg catcaattca aaccacgctg
    22741 gttaataacg aagtaagcaa accactgttt gatatggcaa aaggtgaaac gccttttgag
    22801 attaactcgc gcattggtta cagcggtgat tccagttccg atatttcgct caagccactg
    22861 aattacgagc aaaaggatga aaaagtcgcc tttagcggcg gcgagttcca gttaaatgct
    22921 gacagagacg gcaaagccat ctccctttcc ggggaggcgc aaagtggtcg gatagacgca
    22981 gttaacgaat acaaccagaa agtgcagttg acctttaata atctgaaaac cgacggttcc
    23041 agcacgctgg caagttttgg tgagcgcgta ggaaaccaaa aactgtcact ggaaaaaatg
    23101 accatttcag tggaaggcaa agaactggca ctgctggaaa gcatggagat caacggtaaa
    23161 tcggatctgg tcaatgacgg taaaacgatc aatagccaac tggattactc gctaaacagc
    23221 ctgaaggtac agaatcagga tctgggcagc ggcaagctga ctttaaaagt cggccagatt
    23281 gatggtgaag cctggcatca gtttagccag caatataacg cgcaaactca ggcgctgctg
    23341 gcacagccag aaattgccaa caatcccgaa ctttatcagg agaaagtgac ggaagccttc
    23401 tttagcgccc tgccgctgat gttgaaaggc gatccggtga ttactatcgc gccgctaagc
    23461 tggaaaaaca gtcagggtga aagtgcgctg aatctgtcgc tgttcctgaa agatccggca
    23521 acgactaaag aagcgccgca aacgctggcg caggaagtag atcgttcggt taaatctctg
    23581 gatgcgaaac tgaccattcc ggtggatatg gcaactgagt ttatgactca ggtagcgaag
    23641 ctggaaggtt atcaggaaga tcaagcgaaa aaactggcga aacagcaagt tgaaggtgca
    23701 tcagcaatgg ggcagatgtt ccgtctgacc accttgcagg acaataccat caccaccagc
    23761 ctgcaatata ctaacggtca gataacgtta aacgggcaga aaatgccact ggaagatttc
    23821 gttggtatgt ttgcaatgct ggcattaaat gttccggtcg tacccgctat tccgcagcag
    23881 taattcacag ccaaaacgcc ggagattttt ctctccggcg ttatttttta cttcagcata
    23941 aagtcatact gaatactcat tttcacatcc cagtaattca ggcgatcgcc cagcgtactg
    24001 ccatcgcgct tttcccgccc tttatcggca gcaagaaaga aattcatccc ttgtaacggc
    24061 ccttcgctga cccgatagtt cataccacct aagatacccg cgccttcaat ccgcacatct
    24121 cgtttactgg attcatagcc gcgagtcaca atcggtgcaa atatcagtgt aaattgcggc
    24181 gttaaacgat agttgacgcc caattgccag gactgcattc cttcacggtt acgatcaaga
    24241 cttaatgaaa agggatagcc aatgtcggta tccaccacgg caccattcat tgcatggcgc
    24301 aaataaccac cactgccaaa tacgcttccc cacggcgcat tccaggtcaa ttgtccacta
    24361 accaacgccg tttcattggg ctggctggta ttacgactta aaccttcgag ctgcgcgtaa
    24421 aagccgagca actccccttt caacgtataa ttcgggtcga gcggtatgct tttctcgaga
    24481 atactttgta tctgattgga aacatctttc tgtaccgatg cccccagaga atagcgactg
    24541 gtatcatcat gccaactggc ggctaaaaaa gaacgtgcgc gatgtttatc gtattttccc
    24601 gtctggctgt tatacacccc ttcatcacga ataccggttg ttgaatcagt ccataaaaat
    24661 actcgtgaac gccacattcc ccacagatca atatttggtg ttaaagccca ataacctaat
    24721 gctccttcag aaactgtcgg taatgtacgt tgcgaactgc cagccattaa cggaatacga
    24781 taaggtaagc taccataact ataatccgtc ccgaagcgac ctaatttaag caggctattt
    24841 tcactgggtt ttatttttac tgcccccagg gcaaagccaa aacttttatc accatcaaga
    24901 taccaccggg tactcatatc agcacgagca cctaatttat aaacataata cgccccgcct
    24961 tcaacgccaa taatattgga ataataacca ctattgaaat cgagcaatcc tccctgtacc
    25021 catgcgtaaa tatctcttcc cgcgccagca ctgggcttat ccgccctgcg caattcgttt
    25081 ttcagtctga tgcgtagtcc cgcttcatcg tcagccagtt gcgccgcata agcagaggta
    25141 aggccagagg cagccgtcag gcaaataacg gccatggcca ctatttttct catagttgca
    25201 cctttgatgt tctgattatt tttattgaat attaattagt gatatcgctg attaattgct
    25261 gctgcacttt tttacgatta tcaatttcaa ccacgatttc tttgaatttt ttatccgtga
    25321 gcggataaaa ccagataata acgaatgcca gtagcataaa tccgcaaggt actaaggcaa
    25381 ttgatgtgcg gatgcccata ataacttccg gcgtttgcac ctgattggcg atatatccgc
    25441 ttaatcccaa aataaaggca ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa
    25501 atgagaatag tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt
    25561 ctacggtatc agcttccagt gcccacatca cagtcatagt aacgccctga ccaattgaag
    25621 cgatggccaa cgcaaccaac gccaccggca gtgaccagac ggaaacccag aagaatagca
    25681 gataaccgca ggttcccagc aaagcgccaa tcaggaaggt attcttttta ccgatcctcg
    25741 cgaccatccc cggcaccagc ggtgccgatg ccacagtacc aaccaggttt tgcaccagta
    25801 ccagcacagt gaacagcccg gtatcattta acacatagcg cacgtagaac aacgacgagg
    25861 cgctgaccgc gaaggtcgaa atcagcacac acagcgcacc gatgcacaac ataaacagcg
    25921 ggcgattccg tttcagggtt tgcagactga tcttcaatga cggttgcgcc acgatacgta
    25981 ccacattctc acgcgtcgat ttgaagcaga tgaagtaaag caccattccg gcaatcgcca
    26041 gcacaatcgt ccagaaatgg tataccgaca ccatctcttc cgggctggag ttcttaatgc
    26101 tcggtcctat cagaaatgcc aggcagacaa aggttaatga agcggcaatc ccgcgagccg
    26161 cgcccagacg ggcgcgggat tgtggttgtt gggtcatcgc ggtagcaaat gaaccataag
    26221 gaatattcac caggctgtag caaagcccga ggcccatgta ggtcaaatat gcatacacta
    26281 ctttgctgct atggctccag tcggtcggta cccagaatac cagcacgctg aagatcatta
    26341 acggcgcagt accgaagagt aaaaacgggc ggaattttcc ccagcgggta ttcacactgt
    26401 ccaccactcg tccggcaaag acgtcggcga aggcatcgaa tacccgcacc agtaacagca
    26461 tggtgcccgc cgcagcggca ccgacgccag cgacgtcggt gtagtaactc aacaggaaga
    26521 gcgcccccat tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag ccgacgatgg
    26581 tgcgccagga gagttgttga ttcattgttt gcctccctgc tgcggttttt caccgaagtt
    26641 catgccagtc cagcgttttt gcagcagaaa agccgccgac ttcggtttgc ggtcgcgagt
    26701 gaagatccct ttcttgttac ctccaacgcg caatatgcct tgcgaggtcg caaaatcggc
    26761 gaaattccat acctgttcac cgacgacggc gctgacgcga tcaaagacgc ggtgatacat
    26821 atccagccat gcacactgat actcttcact ccacatgtcg gtgtacattg agtgcagccc
    26881 ggctaacgta tccacgccgt attcggtgat gataatcggc tgatgcagtt tctcctgcca
    26941 ggccagaagt tctttttcca gtaccttctc tgccgtttcc aaatcgccgc tttggacata
    27001 ccatccgtaa taacggttca ggcacagcac atcaaagaga tcgctgatgg tatcggtgtg
    27061 agcgtcgcag aacattacat tgacgcaggt gatcggacgc gtcgggtcga gtttacgcgt
    27121 tgcttccgcc agtggcgcga aatattcccg tgcaccttgc ggacgggtat ccggttcgtt
    27181 ggcaatactc cacatcacca cgcttgggtg gtttttgtca cgcgctatca gctctttaat
    27241 cgcctgtaag tgcgcttgct gagtttcccc gttgactgcc tcttcgctgt acagttcttt
    27301 cggcttgttg cccgcttcga aaccaatgcc taaagagagg ttaaagccga cagcagcagt
    27361 ttcatcaatc accacgatgc catgttcatc tgcccagtcg agcatctctt cagcgtaagg
    27421 gtaatgcgag gtacggtagg agttggcccc aatccagtcc attaatgcgt ggtcgtgcac
    27481 catcagcacg ttatcgaatc ctttgccacg caagtccgca tcttcatgac gaccaaagcc
    27541 agtaaagtag aacggtttgt ggttaatcag gaactgttcg cccttcactg ccactgaccg
    27601 gatgccgacg cgaagcgggt agatatcaca ctctgtctgg cttttggctg tgacgcacag
    27661 ttcatagaga taaccttcac ccggttgcca gaggtgggga ttcaccactt gcaaagtccc
    27721 gctggtacct tgtccagttg caaccacctg ctgatccgca tcacgcagtt caacgctgac
    27781 atcaccattt gctaccacct gccagtcaac agacgcgtgg ttacagtctt gcgcaacatg
    27841 cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg gtgtagacca ttacgctgcg
    27901 atggatcccg gcatagttaa agaaatcatg gaagtaagac tgctttttct tgccgttttc
    27961 atcggtaatc accattcccg gcgggatagt ctgccagttc agttcgttgt tcacacaaac
    28021 ggtgatacgt acacttttcc cggcaataac atacggcgtg acatcggctt caaatggcgt
    28081 atagccgccc tgatgctcca tcacttcctg attattgacc cacactttgc cgtaatgagt
    28141 gaccgcatcg aaacgcagca cgatacgctg gcctgcccaa cctttcggta taaagacttc
    28201 gcgctgatac cagacgttgc ccacataatt acgaatatct gcatcggcga actgatcgtt
    28261 aaaactgcct ggcacagcaa ttgcccggct ttcttgtaac gcgctttccc accaacgctg
    28321 atcaattcca cagttttcgc gatccagact gaatgcccac aggccgtcga gttttttgat
    28381 ttcacgggtt ggggtttcta caggacgtaa cataagggac tcctcattca aataaaaata
    28441 ctggtcaacc tttaatctga ttagttaacc aattctgtgt tgcacacagt tatctgtgag
    28501 aaacccatgg aagagaaatg gaaataaaaa gaactgacac cgcgatcaaa aaaccaaaat
    28561 ctgcaatacc cacagcgtga tattgatcgc attaatcttt aaaacagcgt tgacagaagc
    28621 cataaaataa gcaatcgaac gttcgttcga gcatcttgag aacgataaac tctcatgaca
    28681 tatggctacg gtgaatattt ttggggggaa ttctccgatc ccggctagat tgtattttgt
    28741 acagggaaat taaagtatga aacgcgggca cagttatcac cagcccgcga aatgagagga
    28801 tcaggatgcg gttaagatac cgccaatcat cgcgcgtaat ccctgagcaa acgacgcttc
    28861 acgcgccagg tcttccgctt cgatatcggc cagtacaccg taggtcagat ccagcagtaa
    28921 acgtgaagtc attgacgtgt tgatgtctgg gcttatctcg cctttttgct gggcatcacg
    28981 catccgctgg gcaacaaatt cagtgatcgt catatgctta tttttaagca tggcggcgac
    29041 ctgtgggtta cgcatccctt ccgccataat ttcgaccacc agcgcccgtt gcccaaaggc
    29101 ttcatgggtg agagagacaa tagactcgac catatagtca acaaaatgaa tcccttcaat
    29161 cggttcccgg aaacgggcca gcgccctctc ctggtcctgt aagataatcg cctgaatcaa
    29221 ggcttctttg gagatgaaat ggtgatagag cgtcccggga ctaatggcgc aggatttaca
    29281 gatggctttc atcgaggcac tgtgaaatcc attttctgaa aaaatctctc tggcagcatt
    29341 gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg ttatccatca ttaaaccatt
    29401 atagttagcg tgaataacta gtttaccgcg ttgtcaggtg agcgcctatc attgctgttg
    29461 aatagtgcga aggcacactc tattcgtatg cttttcatcc agctattggt taaccaatag
    29521 ccgtgatttg gctgccagtt gtttagcaga gacgttgatg aattgcacac gctatcccgg
    29581 taaacaacac atacggctgt ttaccggttc gttagtgtat taattgagct cctgtacccc
    29641 accaccggag acggtgagaa tttgtccgct tacccagctg gctgcaggcg agcaaaggaa
    29701 cagcgccgcg ttagcaatat cttgcggttg gcccagacgt ctgatcggcg tgtgctgtaa
    29761 cattttttgt tcaatttctg gtgtaataac ggatttcagg gcatcggtta atattgcccc
    29821 cggcgcaatg ccattcaccc gaatattttt ttcacccagg tcaaatgcca tatttctgac
    29881 cagatgactg gccgcagctt tagatgatgc ataggaagtc atgtttatat ttttattttc
    29941 tgccgccata gaagtgatgg tcagaataac gccaccgcca tttttttcca tttctggcgc
    30001 aacaagttgt gacagatgga aaaaagaaaa cacattcagt tcataagcac ggcgaaaatc
    30061 tgccattggc atatcaaacg gtttaggacc accgccaccg gcgttgttaa ccaggatatc
    30121 aactttaccc agcttactga cggcaaagtc tgccagtgca gagagttcct gttcggaagt
    30181 aatatcacaa cggcaggcaa atgcctgacc acccagttgt tgaatttcgt ctacaacatg
    30241 gttagctgcg tcggcgttaa tatcactgac caccacagat gcgccagctg tcgcgaatgt
    30301 aatggcgatt tctttaccaa tacctgcacc cgcacctgtg atgatggcgc attttccgtc
    30361 gagtctcagg ttgtcagaat taaacacata aacctcctgt gaacttcagt aaaaataaaa
    30421 ggcaccgcct gtgtgctaca ttttttatca ttaaccttga ggaaaggaca ctaccaggta
    30481 tagcagataa agagaggatt atttcgccgc aatgagccgg gcgggaataa taagattgcg
    30541 tgaatgcgtc tcttcatggg tgattttttg catcatgcga tccgcaagtg tggtaccaag
    30601 ttcccgcgca ggcgtgctgg cccaggtaac gggtatatca tcaagtgtgg ttggtgtcgc
    30661 atcggtaaat gccgccagcg aaacttgttg ctcaaagtaa cgatcgactc cgctttcccc
    30721 gctttgccgc cctgctttga gcaaaccaaa ccatgccccc atcgcaatag tttcgttata
    30781 gcaaaccaca gcactgatgg tcgggttatg acgtaaaagc gccgtgatag cttccgcggc
    30841 ttgcttctgg ctggatgtgc actccagcac ccaatcgctg tgaaacggca ggccaaattt
    30901 tagtagagtt gcacaatagc cccccacccg ttctgcacgg gttaatgagg aactttgccc
    30961 tcccagccag gcgatccgct gatgcccatt gcgaatgaga tgctccgtca acaactgtgc
    31021 agcctgcatg ttgtccgggc gaaccgtatc aacatcatca agataactgg cacgggaagc
    31081 gaaaatcacc gggatagctt tttcttctgc catccgtcgc aggtcatcgc tgcttcctgc
    31141 agccccggca attaccacac catccacacc ctgattcagt aacagtgaaa accgctgtgc
    31201 cagctgctcg ccgtctttac cgccgtgaag caaaaaaacc atccgtccct gcgcttccag
    31261 agcttccgtc aatccggccg tcaattcggc gtaaaacggc gcagataaat cacggacgat
    31321 caaaccaatg acgccgcttt gcccgccgcg cagcgccgac gcctggcgat tgcgcacaaa
    31381 tcccagctct tcaatggcgg cgttaacgcg ttctcctgtg gcggtagaga ttcgcccttt
    31441 gccactaagc accagcgaaa cggtacttac cgacacgccc gcagccagcg caacatcatg
    31501 aatggttatt tttttggcgg tagccatgca gatgacctac tccctgatta tgtgacagat
    31561 aaaacgtttt accttttatt tatcttatac ccgctattat cgttgcgtaa tgtgatttat
    31621 gcctcactaa aatttgataa aacgttttat cttctcgcgc aatttactga atccagattg
    31681 ttctctacga ggagtcgttt tatgacggcg aaaacagcac cgaaagtcac gctgtgggag
    31741 ttcttccagc agttaggcaa aaccttcatg ttacccgtgg cattattgtc gttctgcggc
    31801 attatgctcg gcattggtag ttctcttagc agccatgatg tcataaccct gatcccggtc
    31861 ctgggcaacc ccgtgttgca ggctatcttt acctggatga gtaagattgg ctcgtttgct
    31921 tttagtttcc tgcctgtcat gttctgtatc gccatcccgc tgggcctggc acgcgaaaat
    31981 aaaggcgtag cggcattcgc tggcttcgtc ggttatgcgg taatgaacct cgcggtaaac
    32041 ttctggttga ccaataaagg cattctgcca accacggatg ccgcggttct gaaagccaat
    32101 aacatccaga gcattcttgg gatccagtcg atcgataccg ggatcctcgg tgcggtgatc
    32161 gccggtatta tcgtctggat gctgcatgag cgtttccata atatccgcct gccggatgcg
    32221 ctggcattct tcggcggtac gcgcttcgta ccaattatct cctcgctggt gatgggcctt
    32281 gtcggcctgg tgattccatt agtctggccg attttcgcca tgggtattag cggcttgggc
    32341 catatgataa acagcgcggg tgatttcgga ccgatgctgt ttggtaccgg tgaacgtctg
    32401 ctgttgccgt ttggtctgca tcacattctg gtagcattaa ttcgctttac cgacgcaggc
    32461 ggcacgcagg aagtctgcgg tcaaaccgtc agcggcgcac tgaccatctt ccaggcgcaa
    32521 ttgagttgcc cgaccactca cggtttttct gaaagcgcca cgcgtttcct ttcgcaaggt
    32581 aaaatgcctg cgtttctcgg cggtctgccg ggtgcagcgt tagctatgta tcactgcgcg
    32641 cgcccggaaa atcgccataa aattaaaggt ctgctgattt ctggcctgat cgcctgcgtc
    32701 gttggcggca ctaccgaacc gctggaattc ctgttcctgt tcgtagcgcc agttctgtat
    32761 gtcatccacg cgctgttaac cggcctcggc ttcaccgtca tgtctgtgct cggcgtcacc
    32821 atcggtaata ccgacggcaa tatcatcgac ttcgtggtgt tcggtatttt gcatggtctg
    32881 tcaaccaagt ggtacatggt gccagtggtg gcggcaatct ggtttgtcgt ttactacgtc
    32941 atcttccgtt tcgctatcac ccgcttcaat ctgaaaaccc cggggcgcga tagcgaagtt
    33001 gccagctcaa tcgaaaaagc cgttgccggt gcgccgggta aatcaggtta caacgttcct
    33061 gcaatcctcg aagcattagg cggtgccgac aatattgtca gcctcgataa ctgcattacc
    33121 cgtctgcgtt tgtctgtgaa agatatgtcg cttgttaatg tgcaggcact gaaggacaat
    33181 cgggcaattg gcgtagtaca acttaatcaa cataacctgc aggttgttat cgggccacaa
    33241 gttcagtcag taaaagatga aatggccggt ctgatgcata ctgtccaggc ataaggataa
    33301 gatatgttcg atttttcaaa ggtcgtggat cgtcatggca catggtgtac acagtgggat
    33361 tatgtcgctg accgtttcgg cactgctgac ctgttaccgt tcacgatttc agacatggat
    33421 tttgccactg ccccctgcat tatcgaggcg ctgaatcagc gcctgatgca cggcgtattt
    33481 ggctacagcc gctggaaaaa cgatgagttt ctcgcggcta ttgcccactg gttttccacc
    33541 cagcattaca ccgcaattga tacgcagacg gtggtgtatg gcccttctgt catctatatg
    33601 gtttcagaac tgattcgtca gtggtctgaa acaggtgaag gcgtggtgat ccacacgccc
    33661 gcctatgacg cattttacaa ggccattgaa ggtaaccagc gcacagtaat gcccgttgct
    33721 ttagagaagc aggctgacga ctggttttgc gatatgggca agctggaagc cgtgttggcg
    33781 aaaccagaat gtaaaattat gctcctgtgt agcccacaga atcctaccgg gaaagtgtgg
    33841 acgtgcgatg agctggagat catggctgac ctgtgcgagc gtcatggtgt gcgggttatt
    33901 tccgatgaaa tccatatgga tatggtctgg ggcgagcagc cgcatattcc ctggagtaat
    33961 atggcgcggg gagactgggc gttgctgacg tcgggctcga aaagtttcaa tattcccgcg
    34021 ctgaccggtg cttacgggat tatagaaaat agcagcagcc gtgatgccta tttatcggca
    34081 ctgaaaggac gtgatgggct ttcttcccct tcggtactgg cgttaactgc ccatatcgcc
    34141 gcctatcagc aaggtgcacc gtggctggat gccttacgcg tctatctgaa agataacctg
    34201 acgtatatct cagataaaat gaacgccgcg tttcctgaac tcaactggca gatcccgcaa
    34261 tccacttatc tggcctggct tgatctacgt ccgttgaata ttgacgacaa cgcgttgcaa
    34321 aaagcgctta tcgagcaaga aaaagtcgcg atcatgccgg ggtataccta tggtgaagaa
    34381 ggtcgtggtt ttgtccgtct caatgccggc tgcccacgtt cgaaactgga aaaaggtgtg
    34441 gctggattaa taaacgccat ccgcgctgtt cgctaacccc aattgcgcaa cgtaaaaaat
    34501 cgttgcgcaa tcgtggattt ttaccctgct ttgtttttat aatggtgcgc acttttatat
    34561 tcagaaaaag agtgcgacca tgattgatac caccctgcca ttaactgata tccatcgcca
    34621 ccttgatggc aacattcgtc cccagaccat tcttgaactt ggccgccagt ataatatctc
    34681 gcttcctgca caatccctgg aaacactgat tccccacgtt caggtcattg ccaacgaacc
    34741 cgatctggtg agctttctga ccaaacttga ctggggcgtt aaagttctcg cctctcttga
    34801 tgcctgtcgc cgcgtggcat ttgaaaacat tgaagatgca gcccgtcacg gcctgcacta
    34861 tgtcgagctg cgtttttcac caggctacat ggcaatggca catcagctgc ctgtagcggg
    34921 tgttgtcgaa gcggtgatcg atggcgtacg tgaaggttgc cgcacctttg gtgtgcaggc
    34981 gaagcttatc ggcattatga gccggacctt cggcgaagcc gcctgtcagc aagagctgga
    35041 ggccttttta gcccaccgtg accagattac cgcacttgat ttagccggtg atgaacttgg
    35101 tttcccggga agtctgttcc tttctcactt caaccgcgcg cgtgatgcgg gctggcatat
    35161 taccgtccat gcaggcgaag ctgccgggcc ggaaagcatc tggcaggcga ttcgtgaact
    35221 gggtgcggag cgtattggac atggcgtaaa agccattgaa gatcgggcgc tgatggattt
    35281 tctcgccgag caacaaattg gtattgaatc ctgtctgacc tccaatattc agaccagcac
    35341 cgtagcagag ctggctgcac atccgctgaa aacgttcctt gagcatggca ttcgtgccag
    35401 cattaacact gacgatcccg gcgtacaggg agtggatatc attcacgaat ataccgttgc
    35461 cgcgccagct gctgggttat cccgcgagca aatccgccag gcacagatta atggtctgga
    35521 aatggctttc ctcagcgctg aggaaaaacg cgcactgcga gaaaaagtcg ccgcgaagta
    35581 acaaaatgga tggtgcaaat tgcaccatcc atttttcatg caaggcacaa agtcgcgcga
    35641 tgtttggcgg attcgatgcc cagctcaatc aactccatta cctggattgc ctggcttgcc
    35701 ggaaccggat tttcaccatc gccatttaac gcatcacgaa tagccgcata gtaagccgga
    35761 taattcccag gcaccgtcaa cagcgtttct tcgacacgtt cctcaccttc cacgcgggta
    35821 agtacgccat cacgcatatc gtagccccag tcttcctgcg gtagacgctc gccatttttc
    35881 agacgttctt cctgtggatc gaggccatat ttcacataac tgcctcggga tccatgcacg
    35941 atataccgtg ctgactcagc ggctgccagc atggtaccgt gtaaaatgac tcgccgctgt
    36001 ggataggaca agatggcgtg gaaataatcg gtcgactgcg ctccgggccg taactgtgcc
    36061 aaatctaccg tcatgctgac cggtaaacca aaaagcgtaa tggcctgatc aagaagatgt
    36121 ggtgctaaat cgtaccagat accgctgcct gggccgccct gttcacgcca acgatcgcgc
    36181 acctgcggac ggaagcggtc aaaatgagac tcaaagtaag caacttcacc cagcacgcct
    36241 tccgcgagta aaccttttag cgtcaagaaa tcgctatccc agcgacggtt atggaataca
    36301 gacagcacac gccccaggct ttttgccagc gcatccagct ctcgcgcttg tgacagtgtc
    36361 acggtaaagg gtttatcaac gaccacatgt ttacccgcct caagcgccgc tttggctaac
    36421 gggaaatggg tatcgttggg tgtaggaatg acaatcaggt ctatgttggg atcgttaaac
    36481 agatgcttcg gctcagagac aaccgtaacc gttggccagt cggcttttac ttttgtttca
    36541 tcactgctgg agattaccgc cagttccagc ccgggcgtgc ccgcaatcag gggcgcatgg
    36601 aaggttttgc tcgcataacc atacccaatc aacccaacac ggatgttgtc gctcatgtca
    36661 ttgcctctca ttgaagtatg atggctattt gacactatcc tttacccacg ctcaacagtt
    36721 taataacctg ccagcaataa gggatgttgt ttaacttaag tcaaaaaaat agcgaatttt
    36781 ccaacgacaa aagctaaata tcgcaaaaac ctcagtaaaa atcttgctgg agctattatt
    36841 gctaagtaac atttaccccc tgaagttaat ggatcaatca agagagatgt gggctgtaat
    36901 gaatcgtctt attgaattaa caggttggat cgttcttgtc gtttcagtca ttcttcttgg
    36961 cgtggcgagt cacattgaca actatcagcc acctgaacag agtgcttcgg tacaacacaa
    37021 gtaagctctg cacttgtgga gcgacatgct gcccgtccgg gtgcatgttt tcacttgtcg
    37081 gatattaaac caggaattta ttatcttgtt cgatgttgtt ggtgattgtc agggatagta
    37141 aagcgcgtta ctcttttcag agcagttttt catccctgaa tatttgccat cagcgcattc
    37201 ttattattgt aataac
//
LOCUS       DAZFWP020000031        36552 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFWP020000031 DAZFWP020000000
VERSION     DAZFWP020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.031"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            412..2433
                     /gene="uvrB"
                     /locus_tag="ACHK0Z_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             412..2433
                     /gene="uvrB"
                     /locus_tag="ACHK0Z_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU4501459.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2625..3533)
                     /gene="yvcK"
                     /locus_tag="ACHK0Z_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             complement(2625..3533)
                     /gene="yvcK"
                     /locus_tag="ACHK0Z_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU4501460.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      3798..3942
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3930..4919
                     /gene="moaA"
                     /locus_tag="ACHK0Z_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             3930..4919
                     /gene="moaA"
                     /locus_tag="ACHK0Z_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU4501461.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            4941..5453
                     /gene="moaB"
                     /locus_tag="ACHK0Z_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             4941..5453
                     /gene="moaB"
                     /locus_tag="ACHK0Z_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU4501462.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5456..5941
                     /gene="moaC"
                     /locus_tag="ACHK0Z_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             5456..5941
                     /gene="moaC"
                     /locus_tag="ACHK0Z_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU4501463.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            5934..6179
                     /gene="moaD"
                     /locus_tag="ACHK0Z_RS07080"
                     /old_locus_tag="pgaptmp_001416"
     CDS             5934..6179
                     /gene="moaD"
                     /locus_tag="ACHK0Z_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU4501464.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            6181..6633
                     /gene="moaE"
                     /locus_tag="ACHK0Z_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             6181..6633
                     /gene="moaE"
                     /locus_tag="ACHK0Z_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU4501465.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            6770..7474
                     /gene="ybhL"
                     /locus_tag="ACHK0Z_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             6770..7474
                     /gene="ybhL"
                     /locus_tag="ACHK0Z_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4501466.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7619..8323
                     /locus_tag="ACHK0Z_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             7619..8323
                     /locus_tag="ACHK0Z_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HGU4501467.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVLEFINGLVW"
     gene            8393..9106
                     /locus_tag="ACHK0Z_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             8393..9106
                     /locus_tag="ACHK0Z_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4501468.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(9142..10098)
                     /gene="ybhN"
                     /locus_tag="ACHK0Z_RS07105"
                     /old_locus_tag="pgaptmp_001421"
     CDS             complement(9142..10098)
                     /gene="ybhN"
                     /locus_tag="ACHK0Z_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU4501469.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10098..11339)
                     /gene="clsB"
                     /locus_tag="ACHK0Z_RS07110"
                     /old_locus_tag="pgaptmp_001422"
     CDS             complement(10098..11339)
                     /gene="clsB"
                     /locus_tag="ACHK0Z_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU4501470.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHYRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(11336..12097)
                     /gene="ybhP"
                     /locus_tag="ACHK0Z_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             complement(11336..12097)
                     /gene="ybhP"
                     /locus_tag="ACHK0Z_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4501471.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            12230..12640
                     /gene="ybhQ"
                     /locus_tag="ACHK0Z_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             12230..12640
                     /gene="ybhQ"
                     /locus_tag="ACHK0Z_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU4501472.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(12602..13708)
                     /gene="ybhR"
                     /locus_tag="ACHK0Z_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             complement(12602..13708)
                     /gene="ybhR"
                     /locus_tag="ACHK0Z_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4501473.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(13719..14852)
                     /gene="ybhS"
                     /locus_tag="ACHK0Z_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             complement(13719..14852)
                     /gene="ybhS"
                     /locus_tag="ACHK0Z_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4501474.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(14845..16581)
                     /gene="ybhF"
                     /locus_tag="ACHK0Z_RS07135"
                     /old_locus_tag="pgaptmp_001427"
     CDS             complement(14845..16581)
                     /gene="ybhF"
                     /locus_tag="ACHK0Z_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4501475.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(16574..17569)
                     /gene="hlyD"
                     /locus_tag="ACHK0Z_RS07140"
                     /old_locus_tag="pgaptmp_001428"
     CDS             complement(16574..17569)
                     /gene="hlyD"
                     /locus_tag="ACHK0Z_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU4501476.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(17572..18243)
                     /gene="cecR"
                     /locus_tag="ACHK0Z_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             complement(17572..18243)
                     /gene="cecR"
                     /locus_tag="ACHK0Z_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU4501477.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18472..19836
                     /gene="rhlE"
                     /locus_tag="ACHK0Z_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             18472..19836
                     /gene="rhlE"
                     /locus_tag="ACHK0Z_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU4501478.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(20068..20550)
                     /gene="ybiA"
                     /locus_tag="ACHK0Z_RS07155"
                     /old_locus_tag="pgaptmp_001431"
     CDS             complement(20068..20550)
                     /gene="ybiA"
                     /locus_tag="ACHK0Z_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HGU4501479.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            20670..22820
                     /gene="dinG"
                     /locus_tag="ACHK0Z_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             20670..22820
                     /gene="dinG"
                     /locus_tag="ACHK0Z_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU4501480.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            22848..23810
                     /gene="ybiB"
                     /locus_tag="ACHK0Z_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             22848..23810
                     /gene="ybiB"
                     /locus_tag="ACHK0Z_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU4501481.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            23951..25036
                     /gene="hcxB"
                     /locus_tag="ACHK0Z_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             23951..25036
                     /gene="hcxB"
                     /locus_tag="ACHK0Z_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU4501482.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            25103..25162
                     /gene="ybiE"
                     /locus_tag="ACHK0Z_RS07175"
                     /old_locus_tag="pgaptmp_001435"
     CDS             25103..25162
                     /gene="ybiE"
                     /locus_tag="ACHK0Z_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU4501483.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(25265..25525)
                     /gene="ybiJ"
                     /locus_tag="ACHK0Z_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             complement(25265..25525)
                     /gene="ybiJ"
                     /locus_tag="ACHK0Z_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU4501484.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(25790..26056)
                     /gene="ybiI"
                     /locus_tag="ACHK0Z_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             complement(25790..26056)
                     /gene="ybiI"
                     /locus_tag="ACHK0Z_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU4501485.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(26130..26807)
                     /gene="ybiX"
                     /locus_tag="ACHK0Z_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             complement(26130..26807)
                     /gene="ybiX"
                     /locus_tag="ACHK0Z_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU4501486.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(26849..29131)
                     /gene="fiu"
                     /locus_tag="ACHK0Z_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             complement(26849..29131)
                     /gene="fiu"
                     /locus_tag="ACHK0Z_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU4501487.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(29395..29655)
                     /gene="mcbA"
                     /locus_tag="ACHK0Z_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             complement(29395..29655)
                     /gene="mcbA"
                     /locus_tag="ACHK0Z_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU4501488.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            29931..30857
                     /gene="rlmF"
                     /locus_tag="ACHK0Z_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             29931..30857
                     /gene="rlmF"
                     /locus_tag="ACHK0Z_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU4501489.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(30854..33079)
                     /gene="ybiO"
                     /locus_tag="ACHK0Z_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             complement(30854..33079)
                     /gene="ybiO"
                     /locus_tag="ACHK0Z_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU4501490.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(33196..33918)
                     /gene="glnQ"
                     /locus_tag="ACHK0Z_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     CDS             complement(33196..33918)
                     /gene="glnQ"
                     /locus_tag="ACHK0Z_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU4501491.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(33915..34574)
                     /gene="glnP"
                     /locus_tag="ACHK0Z_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     CDS             complement(33915..34574)
                     /gene="glnP"
                     /locus_tag="ACHK0Z_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU4501492.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(34713..35459)
                     /gene="glnH"
                     /locus_tag="ACHK0Z_RS07225"
                     /old_locus_tag="pgaptmp_001445"
     CDS             complement(34713..35459)
                     /gene="glnH"
                     /locus_tag="ACHK0Z_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU4501493.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(35863..36366)
                     /gene="dps"
                     /locus_tag="ACHK0Z_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /gene_synonym="pexB"
     CDS             complement(35863..36366)
                     /gene="dps"
                     /locus_tag="ACHK0Z_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU4501494.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         8701 a         9369 c         9702 g         8780 t
ORIGIN      
        1 tattcctgtg gataaccatg tgtattagag ttagaaaaca cgaggcaagc gagagaatac
       61 gcggcttgca cgcgaattgg cgttaaagac ggctcaaaga aatatctttt attttttaac
      121 tggttagata aatgcaatgg cagtcactga acaggcatct cttgccataa aactgtcatc
      181 actcatcttg acaaatgtta aaaaagccgt tgctttgggg ataacccggt aaggccggag
      241 ttttatctcg ccacagagta aattttgctc atgattgaca gcggagttta cgctgtcaca
      301 gaaatattat ggtgatgaac tgttttttta tccagtataa tttgttggca taattaagta
      361 cgacgagtaa aattacatac ctgcccgccc aactccttca ggtagcgact catgagtaaa
      421 ccgttcaaac tgaattccgc ttttaaacct tctggcgatc agccagaggc gattcgacgt
      481 ctcgaagagg ggctggaaga tggcctggcg caccagacgt tacttggcgt gactggctcc
      541 gggaaaacct tcaccattgc caatgtcatt gctgaccttc agcgcccaac catggtactt
      601 gcgcccaaca aaacgctggc ggcccagctg tatggcgaaa tgaaagagtt cttcccggaa
      661 aacgcggtgg agtatttcgt ctcctactac gactactatc agccggaagc ctatgtaccg
      721 agttccgaca ccttcattga gaaagatgcc tcggttaacg aacatattga gcagatgcgt
      781 ttatccgcca ccaaagcgat gctggagcga cgtgatgtgg ttgtagtggc gtctgtttcc
      841 gcgatttatg gtctgggcga tcctgattta tatctcaaga tgatgctcca tctcacggtc
      901 ggtatgatta tcgatcagcg cgcgattctg cgccgactgg cggagctgca atacgctcgt
      961 aatgatcaag cattccagcg tggtactttc cgcgttcgtg gcgaggtgat tgatatcttc
     1021 ccggcagaat cggatgacat tgcacttcgc gtggaactgt ttgacgagga agtggaacga
     1081 ttgtcgttat ttgacccgct aaccgggcag attgtttcca ccattccacg ttttaccatc
     1141 tacccgaaaa cgcactacgt cacaccgcgc gagcgcatcg tacaggcgat ggaggagatc
     1201 aaagaagagc tggccgccag acgcaaagtg ctattggaaa acaacaaact gctggaagag
     1261 cagcggctga cccagcgtac ccagtttgat ctggagatga tgaacgagct gggctactgt
     1321 tcggggattg aaaactactc gcgcttcctc tccggtcgtg gaccgggtga gccaccgccg
     1381 acgctgtttg attacctccc tgccgatggg ctgctggtgg tcgatgaatc tcatgtcacc
     1441 attcctcaaa ttggcggcat gtatcgcggt gaccgggcgc gtaaagagac actggtggag
     1501 tacggcttcc gcctgccatc agcgctggat aaccgtccgc ttaagtttga agagttcgaa
     1561 gcattagcgc cgcaaaccat ctatgtttcg gcgacgccgg gtaattacga gctggaaaaa
     1621 tccggcggcg atgtggtgga tcaggtggtg cgtccaaccg gattgcttga cccgattatc
     1681 gaagtgcggc cggtggcgac acaggttgat gatcttcttt cggagattcg tcagcgagcg
     1741 gcaattaacg aacgcgtact ggtcaccaca ctgaccaagc ggatggcgga agatcttacc
     1801 gaatatctcg aagaacatgg cgagcgcgtg cgttatcttc actcagatat cgacaccgtc
     1861 gaacgtatgg agattatccg cgacttgcgt ctgggtgagt tcgacgtgct ggtagggatc
     1921 aacttactgc gcgaaggtct ggatatgccg gaagtgtcgc tggtggcgat cctcgacgct
     1981 gacaaagaag gcttcctgcg ttccgaacgt tcgttgatcc agaccattgg tcgtgcggca
     2041 cgtaacgtta acggtaaagc gattctctac ggcgataaga tcaccccatc aatggcgaaa
     2101 gcgattggcg aaaccgaacg tcgccgtgag aaacagcaga agtacaacga ggaacacggc
     2161 attacgccgc aaggcttgaa caagaaagtg gtcgatatcc tggcgctggg gcagaacatt
     2221 gccaaaacca aagcgaaggg cagaggaaaa tcgcgcccga ttgttgagcc ggataatgtg
     2281 ccgatggata tgtcgccgaa agcgttgcag cagaaaatcc atgaactgga agggctgatg
     2341 atgcaacacg cgcagaatct ggagttcgaa gaagcggcac aaattcgtga ccagttgcat
     2401 cagttgcgtg agttgtttat tgccgcatcg taacaggata gcgaagaaga ctgatgacaa
     2461 acggaaaaca gcctgatgtg ctacgcttat caggcctaca ttttctccgc aatatattga
     2521 atttgcgcgg tttgtaggcc ggataaggcg ttcacgccgc atccggcata aacaacgcgc
     2581 actttgtctt caataggaag ccggaatttt ccttcggatt tccgttaacc taaagcctgt
     2641 aacgcctttt ccagcgcgtt atgtaacaac tggcggtcat gacgatacgg aatatcgctg
     2701 gcctccagta cctcctggat cacaatccgc tctttcaccg ccgagacatc cacttttggc
     2761 ccgacgatga ccgcatcaat gaccttttta ccaacatact gctccataat tgccagcttg
     2821 ctttccagct tcaaattagc cgcaggtaaa ctcaactcac gccccagatt gccgatataa
     2881 accatcggcg ctggcgtgcg gcgtaatgcc tgggcgattt ccttcagcag cagaattggc
     2941 atcaggctgg tataaaaact gccaggccca ataatgatga gatccgcttc attgatagcg
     3001 tgaaccgcct cacgcgttgc gggtacatta ggcgttaaca ataactcttg aatcggcgta
     3061 gttaactggt cgatattgac ctcgccgtaa acttcatgcc cctgatcgtc aatcgccatc
     3121 agatcaacag gatgctctga cattggaatc aaatgcgtat ccactttcag cagattacga
     3181 attaaattga tggcttccag aggccgcacg ctaaggtgat ccagcgcctt taacatcaag
     3241 tttccgagat tatgaccgga aagttcgcca ttgccaccaa aacggtattc aaacatcgcg
     3301 gaggcgacgc tcggttccgt tatcagctgg ttgaggcagt tgcgcatatc gccccaggca
     3361 atgccgcctt ctgaacggcg aatacgcccc gtcgagccac cattatcggt ggtggtgacg
     3421 atacccgtta aacgagaacc caaagacgaa agtgatgaga gaacgcgtcc cagtccatgc
     3481 cctccgccga gagcaacgac acgatcaaga tcagccagcg tacgattgcg catatagttt
     3541 cctggagtca gattatccgc gctacagtag cgcaaattgc ggggaaacag caattaacct
     3601 gccaatattg aggatacaaa ctattttctt ctacctctaa aggacgatgc acgctatgcc
     3661 tccctgatga tgtatatcaa agtaaagccg ccattttccc ttattctgta gcgaaatagc
     3721 acgatcatga cgctatatac atgattacat agcgaaagtg tggatggtaa aaatctcatt
     3781 tacacgctag tatcggcata accactaaac actctagcct ctgcacctgg gtcaactgat
     3841 acggtgcttt ggccgtgaca atgctcgtaa agattgccac cagggcgaag gaagaaatga
     3901 cttcgcctcc cgtatctgga aaggtgtaca tggcttcaca actgactgat gcatttgcgc
     3961 gtaagtttta ctacttgcgc ctgtcgatta ccgatgtgtg taactttcgt tgcacctact
     4021 gcctgccgga tggctacaaa ccgagcggcg tcaccaataa aggctttctt accgtcgatg
     4081 aaattcgccg ggttacgcgc gccttcgcca gtctgggcac cgaaaaagtg cgcctgacag
     4141 gcggtgagcc gtctttacgc cgcgacttta ccgatatcat cgccgctgtg cgggaaaacg
     4201 acgctatccg ccagattgcg gtcaccacca atggttaccg tctggaacgc gatgtggcga
     4261 actggcgcga tgcgggactt actggcatta acgtcagtgt cgacagtctg gacgcccgcc
     4321 agtttcacgc tattaccggg caggataaat tcaaccaggt catggcgggg attgatgctg
     4381 catttgaggc cggttttgag aaggtcaaag tcaataccgt gctgatgcgt gatgttaatc
     4441 atcaccagct cgacaccttt ctgaactgga tccagcatcg ccctatccag ctgcgtttca
     4501 tcgaactgat ggaaacgggc gagggcagcg agctcttccg taagcatcac atctctggtc
     4561 aggttctgcg tgacgagcta ctgcgtcgcg gctggatcca ccaattacgt caacgcagcg
     4621 acggtcccgc gcaagtcttt tgccatccag attacgccgg agagattggc cttatcatgc
     4681 cgtatgaaaa agacttctgc gccacttgca accgcctgcg cgtttcctcc attggtaaac
     4741 tccatctctg cctgtttggt gaaggcggcg ttaacctgcg cgatctgctg gaagacgata
     4801 cccagcaaca ggcgctggaa gcgcgtattt cagcggcgct gcgggagaag aaacagaccc
     4861 atttcctgca tcaaaacaac accggtatta cgcaaaactt atcgtacatt ggcggctaaa
     4921 acgtcaaaag gagagatcag atgagtcagg taagcactga atttatcccg acccgtattg
     4981 ctattcttac ggtttctaat cgtcgcggtg aagaagacga tacctccggt cactatctgc
     5041 gcgattcggc gcaagaagcg ggccatcacg ttgtcgataa agccattgtg aaagaaaacc
     5101 gctacgctat tcgcgctcag gtatctgcgt ggatcgccag cgacgatgta caagtggtat
     5161 tgattacggg tggtactggc ctgacggaag gtgatcaggc tcccgaagca ttgctgccgt
     5221 tgttcgaccg tgaagttgaa ggttttggtg aagtgttccg gatgttgtct tttgaagaga
     5281 ttggcacttc cacgttgcaa tctcgtgcgg tagcgggcgt cgccaacaaa acgctgattt
     5341 tcgccatgcc gggttcgacc aaagcgtgcc gtaccgcatg ggaaaatatc atcgcgccgc
     5401 agctggatgc ccgtacgcgt ccgtgtaatt tccatccaca tttgaagaaa taagtatgtc
     5461 gcaactgacc catatcaacg ccgctggcga agcgcacatg gtggatgtct ccgccaaagc
     5521 ggaaaccgtg cgtgaagcgc gggcggaagc ctttgtcacc atgcgcagcg agacgctggc
     5581 gatgattatt gatggtcgcc accacaaagg cgacgtattt gccactgcgc gtattgccgg
     5641 tattcaggcg gcaaaacgca cctgggatct gatcccgctc tgtcatccgc tgatgctcag
     5701 caaagttgaa gtcaatttac aggccgagcc ggagcacaat cgggtgcgta tagaaacctt
     5761 atgccgcctg accgggaaaa ccggtgtcga aatggaagca ttaaccgcgg cctccgtggc
     5821 ggcgctgacc atttatgaca tgtgcaaagc ggtgcaaaaa gatatggtga ttggtccggt
     5881 acgtttgctg gcgaagagcg gcggcaagtc gggtgacttt aaggtggaag cggatgatta
     5941 aagttctttt tttcgcccag gtgcgcgagt tggtgggaac agatgcaacc gaagtggctg
     6001 cggatttccc aactgttgaa gcgttacgcc agcacatggc tgcgcagagc gatcgctggg
     6061 cgctggcgct ggaagatggc aaattactgg ctgccgtcaa ccagacgctg gtgagttttg
     6121 accatccgct gactgacggc gacgaagtag ctttcttccc gccggtaacc ggaggttaag
     6181 atggcagaaa ccaaaattgt tgttggtccg cagccgttca gcgtaggaga agagtacccg
     6241 tggctggcgg agcgtgacga agacggtgcg gtagtcacct ttactggtaa ggtgcgcaac
     6301 cataacctgg gcgacagcgt caaagcatta accctcgaac actatccggg gatgactgaa
     6361 aaagcactgg cagaaattgt tgatgaagcg cgtaaccgct ggccgctggg gcgcgtcact
     6421 gtgattcacc gcatcgggga attatggccg ggcgatgaaa tcgtttttgt cggtgtcacc
     6481 agtgcgcatc gcagcagtgc gtttgaagcc gggcagttta ttatggatta tctcaaaacc
     6541 cgcgcaccgt tctggaagcg cgaagccacg ccggaaggcg accgctgggt tgaagcccgg
     6601 gagagcgatc agcaggcggc aaaacgctgg tagttttttg ttagccggat aaggcaccgc
     6661 tgcgtccggc aatttcaccg ctcagtacat acgtttacgc tgcgtcgatg cacagcctca
     6721 tcattttgca gtatccttaa gatattcctt atatcttcag gagatcgtca tggacagatt
     6781 cccacgttct gattcaatcg tacaaccccg ggctggcttg caaacttata tggcgcaagt
     6841 ctatggctgg atgaccgttg gcttgttgct gaccgcattt gttgcctggt atgcggctaa
     6901 ttccgcggcc gtgatggagc tgttgttcac taaccgtgtc tttttaatcg gtctgatcat
     6961 cgcgcaatta gcattggtta ttgtgttatc agcgatgatt caaaagctga gcgcaggtgt
     7021 aacgacgatg ctctttatgc tttattcggc gctgacgggt cttacgcttt ccagtatatt
     7081 cattgtctat accgctgctt ctatcgccag tactttcgtc gttactgccg ggatgttcgg
     7141 cgcaatgagc ctgtacggtt acaccacgaa gcgcgattta agtggctttg gcaatatgct
     7201 gtttatggcg ttaatcggca ttgtgctggc atcgctggtc aacttctggt tgaaaagcga
     7261 agcattgatg tgggcagtta cctacatcgg cgtgattgtc tttgtcggat tgacggcgta
     7321 tgacacgcag aaactgaaaa atatgggtga gcagattgat acccgcgaca cgtcgaacct
     7381 gcgcaaatat tccattcttg gcgcgttaac cttgtatctg gacttcatca acctgttcct
     7441 gatgttgttg cggatcttcg gcaaccgccg ttaaattctc ttcttcctgc tgatcccggc
     7501 ctggagccgg gatttttttc ggattttata aagactattc cgctcattta catgagaact
     7561 gaatcgcggc aaatgccaga ctatgtccac tacgtaattt cagggacaga taacgcattt
     7621 gaacagacaa tccactgcta ctcaattaca gcaaaaaagt gcttcgctca gcaaaatgct
     7681 gcttctcatt tggtggttaa cagcagctgg agtatgggct acggcaggct gtgcctggat
     7741 atttcagcat ttgcattggg ttacgtcaca aaagtctcca ggtgcgtttg tcttcgcttt
     7801 ggctgcgctg ataataggtg gtctctggtt aaacaacgcc ctgaaaagac tgaatgtatc
     7861 aggcgcatgt ctggtctatc tgctaataat ccttcttgtt ggctttctct tcgccagcct
     7921 ttataccttt tcgagcatag cgacggtaat gggaattacg ggagggatgt ttgccgcaat
     7981 ggcgctgatt tgctcttgta gtaatagggt tatcccacca gtcagacagt tatattcata
     8041 tattttctgt ggtttgagta ttgcttttgt tgttaacctg atccttacca gcagtttttc
     8101 cgtgtggtta gcgagtattt tgactgtctt tatctggggc attacggctg cctgtgaagc
     8161 aacaacactg gaagatttga tccgtgttgc cgacacctct gaaatttcag gttctcttcg
     8221 ctgtatcgtt cctggggcaa taacactcta ttttagtatt ttaagcgtcc tctttagaat
     8281 tactgtaaca gttcttgaat ttattaatgg tttagtgtgg tagtgcgaat tgacgagttt
     8341 tattgcgtta attggaatag ataatgatat ttaacttaag gatataaaaa taatgaattc
     8401 gcgctcagca aataacataa ctaatgattt tcagcacgaa gccaggatat taaacagcat
     8461 atggctcatt accgctttag gtttggtgac aaccgcagga ctggcctggg tagcgaagta
     8521 tctcgaaatt acggcaacaa aatatgattc accgcaaatg tatgtcgcca taggattatt
     8581 attactttgt atgtatggct taagtaagga tatcaacaag ataaatgccg tcatcgcagg
     8641 cgtaatatat ctatttttac tctctttggt ggcgattgtc gttgctagtt tagctcctgt
     8701 acctgccatt atcgtcgtgt tcagcactgc gggcgcgatg tttttaatca gtatgctggc
     8761 cggtttatta tttaatgttg atcctggttc tcaccgtttt atcattatga tgacgttgac
     8821 agggttggcc ctggtgatca tcatgaatgc cgcattaatg agtgaacgac ccgtttgggt
     8881 aataagttgc ttaatgattg tgttatggtc aggcattatc tcgcatggac gaaataagct
     8941 ccttgaattg gcggggaaat gccatagtga agagttgtgg agtccggttc gttgcgctgt
     9001 tacaggtgca ttaacactct attactattt tatcggcttc tttgggatac ttgccgcgat
     9061 agctataacg cttgtctggc aaaggcatac gcgttttttt cattagtcgt aaatgcagat
     9121 actaaagaac ttaactaaac ttcacatcgc cgcttcattt ttcgcccgta gcttcttcgc
     9181 ctggctttcc agtagcaggt aacagatcag cgccagcagc agcgggataa agtaatacag
     9241 gacgcgataa gcgagcaggg cggcgataat gctccctttc gaggtatgct ccccagccag
     9301 tagtgcgata aacaccgctt ccagcacacc gatccccgcc ggaatatgca cgatgacgcc
     9361 agcaatacta ctaaccagta acacgcccag tacaaagaaa tagttcacgc tttgaccaag
     9421 taacagccag ataatcgccc ccattaccat ccagttaacg ctggaaatca gcatctgagc
     9481 gagggcgaat ttccatgaag gcagcaccag cttttgccct ttgatggtca tatggcggtg
     9541 cttcgcgaaa gcgcaaaacc acaaataaac cgcgataatc atcagtaagc caatgccgag
     9601 aatacgcagc gtagtttgat cgacatacca gtgatccggc aactccacca cgcctgcggt
     9661 aaagataatc cctgccagta aaatatagcc cagccagttg gtggtaatac tgagcgagaa
     9721 aatccgcgta atagtgctgc ccggtaaccc tagtcgggag tacaaacgat agcgcatacc
     9781 aatgccgccg acccaggtac tgagcgtcag gttgaaggcg tagcagataa acgacaccag
     9841 catcacctgg cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca
     9901 gccgtaaatc agatagctga cgaccaccag cccgaccgca ctaagcagcg caacgcgatt
     9961 gtagtcgcgg atgaccttcc agacctcttc ccagtccact tttttggcgt agaccaccag
    10021 caacacaatc accgcgataa aaaacagcca ggtgaggatc ttctttgcta agcgccagcg
    10081 cgggtgtgat ttactcatca gggttttacc cccgtgtttt cagtttctac ccgatcctgc
    10141 gtttccattg tcggttgtgc gggcggatca acctgcgcca gacgtggtgt gtgtgccgga
    10201 agccagccaa ccagcgccgg gaagtggcgt aaaaagtgga acgccagcac gcttttggtc
    10261 aggttccacc aggtgcgttt cggcagcatg gtctcatcga cctgctgaca atctgcggca
    10321 ataatgccgt tcagattatc gcgcagcgtc tggttaaaat gacgatagtg gatgatgaca
    10381 tttgcttcga gattcagtga caaactgagc ggatcgagat tactggaccc tactgtcgcc
    10441 cagtgatcgt ccatcaatgc cactttgccg tggagcgggc ggcggcggta ctcaaatacc
    10501 tgaacgccgc ctttaaccag atagttatac agcaagcgcg caccgactct gacaatcggc
    10561 atatccggtt cgccctgaat gatcagtttg atccgcaccc cgcgccgtgc cgctttacgc
    10621 aaggcgtgta aaaatcgata gccggggaag aagtaggcgt tggcgataat gacttctcgc
    10681 cgcgcctgag tgagcatttt cagatagtga cgttcaatat catcgcgatg ttcttcgtta
    10741 tcgcgccaga ccagcaatac ctgcgcttct cccggctggc ggttctcttc cgctttgtga
    10801 tgacgtcgcc accagcgtcg tgccgcgctc tgtccaggca ggttttccag ctcaaactgg
    10861 agaatatctt cgacaatcgg cccttcaagg cgtaccgcgt aatcctgttt agcctctgga
    10921 ccgtagctgg acatatgctc ggcggagtaa ttcagcccgc caataaaggc gatacgcgcg
    10981 tcgatcacca caattttgcg atgcatccgg cgaaacacat tggtgcgcat accaaaaagg
    11041 cgagggcggg gatcgtagta gcgaaatacc acgccagctg ccgtcagttc attgacaaac
    11101 tcatcgctga gatccggcga accgtagcca tccagcaaga cttccgcttt aaccccgcgt
    11161 tgcgctgctg ccagtagtgc cgcatgcagc tgtttgccga cgtcatcctc aaaccagata
    11221 aacgtttcaa gaatgatgcg ttcttgtgcc tcgccaatcg ccttaaacac cgcgggataa
    11281 tattgctcgc cgttttccag caactggatc ttattgcctt cgcgccagct acatttcata
    11341 aatgaatctc cgcacttaaa ggggcatgat cagaaaggtg tcgccatgtc cgcagcggca
    11401 acgcggttgg cgcgctggcg ctggcatttt tgacgtagat cctgtccagt cgtagtagag
    11461 gaaattgcac cggaaacgtg cgcgccgggc gtccgtgggc gcgggtaaaa atctcatcca
    11521 gtccggcctg cacttttaac ggatgattag ctttttgccg ccagtcattg aaatcacccg
    11581 ccaccaatac cggttcgccg tccggtagct cattcaccca ttcggcgagc atcgcaagct
    11641 gcgcctgacg gtgcgcctca cgcaggccca gatgtacgca catcacatga atcgcttttc
    11701 cggtcatcgg tggcacaatg cggcagtaga gcacgccgcg cttttccgca ccatcgaccg
    11761 aaacatcgcg attctcataa tgttcaatgg gataacgcga cagtacggcg ttgccgtgat
    11821 gcccttccgg gtatacggca ttgcgaccgt aggcaaaatc gctccacatc gtgtcggcga
    11881 gaaactcgta gtgcgaggta tcgggccagt tttccacatg cagcggatga acttcgtgcg
    11941 cgcccatcac ttcctgcagg caaacaatat cggcgctgac ggtacgcacc gcgtcgcgaa
    12001 gttccggcaa aatgaagcgt cggttaaacg cggtaaaacc tttgtgaatg ttgatggtga
    12061 gcaccttgaa cgaaaattgt tgtgtttgat cgggcataat tttcctgtct ttgcctctta
    12121 tctcattgaa atagtgtagt cggcgtcaca aaaaggtgcg gtcttacgga attttccgta
    12181 aagttcggta ctctgagtaa gtagagataa attcttcagg agagaagcca tgaagtggca
    12241 acaacgtgtt cgtgtcgcaa ctggtctaag ttgctggcag attatgttgc atttactggt
    12301 agtggcgctg ctggtggtgg gctggatgag taagactctg gttcacgtcg gcgtgggatt
    12361 atgcgcactg tattgtgtca cggtagtgat gatgttggtg tttcagcgcc accccgagca
    12421 acgctggcgt gaggtggcag acgtgctgga agagctgacc acgacctggt attttggcgc
    12481 agcgctgatt gtgctgtggc tgttgtcccg tgtactggaa aacaactttt tgctggcaat
    12541 tgcagggctg gcaatccttg ccggcccggc ggtagtgtct ttgctggcga aagataagaa
    12601 gttacatcac cttacgtcta aacatcgcgt acgccgctga ccctgtcgtg gccgttatca
    12661 ccagtagcgg ccacaaacta ttccacacaa tatccagact cgcatccttc aaataaatct
    12721 gcttggtaat gtccgtaaag tggcgaatag ggttaatcca cgtcaggttt tgcagccata
    12781 ctggcatgtt ttccaccgga gaaacgtaac cggaaaggag aatggcgggc atcataaaaa
    12841 caaacacgcc gataaacgcc tgctgttgtg tcgagcagag tgatgaaatc aacagaccga
    12901 atcccaccag cgataaaccg taaatcacca tcgtaaagta gaacagcgcc agcgatccgg
    12961 cgaaggggat ttgatacgcc cagataccaa tcgccagcac aatggtggcc tggaaggtgg
    13021 cgacaattaa cgccggtacg gctttgccga tgaagatctg ccaggtggtg agcggcgaaa
    13081 ccagtagctg atcgagcgta ccttgttcac gttcgcgggc gacggaaagt gaagtgacga
    13141 tcattacgcc gatagtggtg atcatggcga tcagcgacgg caccacaaac catttgtagt
    13201 caagattcgg gttataccag ttgcgtacca ccagctcgct gttgttaggt ttcggttttc
    13261 cttccagcag ctcctgctga taatttttga cgatctgttg caggtagttg gcggcaattt
    13321 gcgcactgtt ggagttacgc ccgtcgagga tcaactgcaa cggcgcggtc tggaaggtat
    13381 ccagtttgcg cgagaagtca gccgggaaac gcaccagtag taacgccttt tgtgtgtcga
    13441 tggttgggcg gatctcctgt gggcttttca gcagcagcac atgagtaaag gcgctggcgc
    13501 gggcaaaacg ttgggtcagc tccaccgaat gctcgccgtt atcttcatcg tagatggcga
    13561 tggtggcgtt agtcacttcc agcgtggcgg cgaacgggaa cagaatcacc tgaataagca
    13621 caggtaaaat cagaatcgcg cgggtttgcg gttcgcgcag cagcgactgc aactctttgc
    13681 ggattaacgt ccataagcga tgaaacatgc tcttctccct aatccagccg acgtttggtt
    13741 ttcagccacg tcaggccgat aaacatcacc gccgaagcga tcaaaaacag cacgtttacc
    13801 accagcacca ctggaatatt cccggcgagg aacaggcttt gcagggtgct gacgaaataa
    13861 cgagcgggaa taatgtacgt caccgcgcgg atcaccgcgg gcatactgtc gatctgaaaa
    13921 ataaagccgg aaagcataat cgacggcaga aaagcggcgt tcagggcgac ctgagcggca
    13981 ttgaactggt tgcgggtaat cgtggaaatc agcagcccca tccccagggt actgagtaaa
    14041 aacaggctgg agataaaaaa cagaatcagc agcgacccgc gatacggcac gccgagaata
    14101 aacactgaca ccagcataca cagcaacatc gccagcatcc cgagaaagta ataagggatc
    14161 agcttacaca gcagcagttc cgtgcgggta atctccgtag agagcagagc ctccatggtg
    14221 ccgcgttccc attctcgcgc caccaccagc gaggtgagaa tcgcgccgat gaccgtcatg
    14281 ataatggtca ccgcaccggg gataatgaag tgctggctaa tcgccgccgg gttaaaccag
    14341 tagcgggttt gtacatcaat aagcggttca aaagtctgcc cgttgtcctc cgctcgctgc
    14401 atttgccaga tctgccagat cccttcgaca tacccctgta caaagttagc ggtattcggc
    14461 tcactgccgt cggtgatcac ctgaatcggt gcggtggcgt tggcgcgctc catctgttcc
    14521 gcaaaatcca ccggaataac caccagaccg cgaattttcc ccgcctgcat tttggcgatc
    14581 agttcctgac ggttatcgct gatggtggcg tcgatgtagg gcgaaccggt catggtgtgg
    14641 gtgaaatcca gcgccgcttc gctacgctgt tccagtaaaa tcccgacccg cagcttgctg
    14701 gagtcgaggt taatgccgta accaaaaata aacagcagca acagcgggat cactaccgca
    14761 atcagccagc tactcggatc gcgaacgatc tgccgcgtct ctttgacgca cagcgcccgc
    14821 acgcgacgcc aggaaaggat cgggttactc attgctatgc tccttatccc agtcgtggat
    14881 caactgaata aaggcttgct ccatggtggg atcgggttgc tcatcgttag ccgactgtgc
    14941 tttcaaatcg tccggcgtgc cgctggcgat taatttcccg cggtacacca ggccgatgcg
    15001 gtcgcaatat tccgcttcat ccataaagtg ggtggtgacc atcaccgtga cgcctttctc
    15061 taccatgctg ttgatgtgca gccaaaattc acggcgggtg agggggtcaa cgccggaagt
    15121 cggttcgtcg agaaacagaa tatccggttc atgcatcagc gaacaggcca gcgccagccg
    15181 ctgtttaaaa cctaatggca gttcatcggt ggcgtgggag gcaatacttt tcaggccaaa
    15241 tgcctcgctc atacgggaga ttttttcgtt ctgcgcccga ccgcgtaagc catacacacc
    15301 agagaaaaag cgtaaattct gttcgaccgt caggttgcca tagagcgaaa acttttgcgc
    15361 catatagccg agatgctggc gcgctttacc ggaactctct ttcagatcca tccccagcac
    15421 cagcgcctga ccggaggtcg gcaccagcaa gccgcacatc atcttaaagg tggtcgattt
    15481 ccccgcgccg tttggcccga gcagaccaaa aatctcccct cgtttaacgg caaagttgac
    15541 gtgatcggtg gcggcaaaat caccaaattt cttggttagt tctttcgctt cgatcaccgt
    15601 ctcgccgggt gtgccttcta ccgtatgcaa tattgcaccc agcggcgatt ccgaggttcc
    15661 ggcaccgccc agcaaatcaa taaacgcatc ttcaaaacgc ggcgtagttt cgttgatgtt
    15721 gatttccggc atcccttcgg catggcgaat atcgtctggt gtggcctctt ttttgaggat
    15781 cagacgtacc gatttcccct gaatcatgcc gtcgctgacc tgtggcagtt tcaaggcgcg
    15841 ttgcaatagt ttgcggttgc cctcgtgtgg actggtcatc agaaaactgc gtccggccat
    15901 ggtttgtgtc agggcttttg gttccccctg atacagcaac tcgccttcgt tcatcagtaa
    15961 cacgtcacgg cactgctcgg cttcgtcgag atacgaggta ctccagagga ttaacatccc
    16021 ttcgcctgcc agctcatgca ccatctgcca cagttcgcgc cgtgagatag ggtcaacgcc
    16081 gacgccgggt tcatcgagca gcaacacttt cggttcgccc accagggtac aggccagacc
    16141 gagtttttgt ttcatcccac cggagagctt gcccgccagg cgtccggtaa acggcccaag
    16201 agacgtaaac tccagcaggc gagcaaaagt ttgcttacgt gcctcgccgg tgacgctgcg
    16261 caaatccgcg tacagattga ggttctccat caccgtgaga tcttcataca gaccaaattt
    16321 ctgcggcata tagccgagca cggcgtgcag cgcgccgtcg tttttgattg gatcaaagcc
    16381 aatcaccgtg gcattgccgc tgtcgggttt cagtaatccc gccagcatcc gcattagcgt
    16441 ggttttacct gcaccatccg gccccaccag ccccgtcaca taaccggcgt gaatggtaca
    16501 atcgagcggc gcgacggcgg gcttgtccat gcccggaaag cgtttttcta ggccgttcag
    16561 cgtgataacg gcatcattca tgtcctgcct cgttaccgaa ttgcaccgtc accggcattc
    16621 cctggcgtaa cgcatcatcg gcgtcggtca ccacaatacg caggcgatag acgaggtcgg
    16681 tacgcagatc cggtgtttcg acggttttcg gggtaaattc agcagtcggc gaaacgaaac
    16741 caatctgtcc gtgatagggc ttgtccgggc gaccatcggt ataaagcagc actttgcgcc
    16801 ccggctgggc ctggtcaaga ttccgttcat caacataggc acgtacccac accggacgcg
    16861 ttagtgaaac ggtaaacacc gtgccacctt cattgagaac cgtgcctggc tccaccgcgc
    16921 gcgttaacag cgtgccatca gacggtgcta tcaacgttga gtcctgtaaa ttcagctccg
    16981 cctgcgccag ttgcgcctgc gcctgttcga ggctggcttt cgcctgagcg atgtcctgtt
    17041 cacggttacc ggaacggtac tgacgcaatt tatcctgtgc tgatttcagc gttgcctgtg
    17101 cctggtcgcg cgaggagcgg gcattttcca ggtcatttgc cgaaatggtg cggcttttcc
    17161 acagtccttg ctggcggtta tagaagttct gcgcatagtc ataggcggct tgagcctgtt
    17221 tcaccgctgc ggcggcctga gcgatttctt catcgcgata cccggcaagc atcagatcat
    17281 actgcgcctg tgccaccgag acgcccgctt tcgcctgcat cagggcaatc tcatacggct
    17341 tgtgatccag ttcgcccagc acctggcccg ctttgatagc atcaccttcg tccaccgcca
    17401 gcgattcaac gcgcccccca acacggaaac taagatttac cgtacgaata tccacgttgc
    17461 catacagcgt caggccgtta tcctggcggc tttgatacca ccagtagcct ccggcaacca
    17521 cggcggcaag taccactacc gccaatccga tcacgacagg ttttttcatc actacaaact
    17581 cctttgcgat aatccttgca aaatcaggtc gatatgacag gtcaccgtct ggttgatcag
    17641 ttcggtcttt tcttcatcga acgcggtcca gccggtacgt aacagaattg tttctttgcc
    17701 aagacggaac gccagaatct caccaatcaa cgcatgagta tggaggatca tgcgggtatc
    17761 gttggcgtcg cagccggtcc aggcggcaat caggcgtgtc aggtggctgt gtagcgggct
    17821 aatcacctgt tcgtgcacca ggtgataggc tgccgtggga gagagctgct cacgggagat
    17881 aaacttgctg aggttgacgg tgtcatcctg ggtgagcagc ttaatcatgt tcctgcaggc
    17941 gcgaaggatc agttcacgga tggctgcgcg atcaggctgt ggttgtgcga acaagcgttc
    18001 ggcctcttcg gcgtgcggac ggaactgctc gccaataaaa tcggcaatcc actgggcgca
    18061 ggcgaggtac aaatcttctt tcgaaccgaa gtagtaggtg atggcagcga tattctgccc
    18121 ggcctgggcg gctatctcgc gggtggtggc gttcattcca tattcaccaa actgcgccag
    18181 tgcggcagca atcagctgtt ttttcgcctg ttcacccttg attgtcatgg caggattatt
    18241 catcgcacag tccattctta atcaaatgat tgattaagat tatgactcca tatgggagtt
    18301 gtccagtatg gctaagaatt ttagctacgc cagtcacagg gataatttat gcgctgcgtc
    18361 acaaaaactg ctacactccg ctccctcatg acattgtggt ttttgtcatt ttccttttca
    18421 gtatctccct gaaaactaca ccggtaacgg tcggggcggt tcggagtagt tatgtctttc
    18481 gattctttgg gtttaagccc tgatatcctg cgcgccgttg ccgagcaggg ttaccgtgaa
    18541 cccaccccta ttcagcagca ggcgatccct gcggtgctgg aaggccgcga cctgatggct
    18601 agcgcccaga ccggtaccgg taaaacagcg ggctttacgc tgccgctgtt gcaacacctg
    18661 atcactcgcc agccgcacgc caaagggcgt cgtccggtac gtgcgctcat tcttaccccg
    18721 acccgtgaac tggctgcgca gattggcgaa aacgtccgtg attacagcaa atatttgaac
    18781 attcgttcgc tggtggtgtt tggcggtgtc agcattaacc cgcaaatgat gaagctgcgc
    18841 ggcggcgttg atgttctggt ggcaacaccg ggacgtttgc tggacctgga acatcaaaat
    18901 gcagtgaagc tggatcaggt tgaaatcctc gtcctcgatg aagctgaccg catgctcgac
    18961 atgggcttta tccacgatat ccgtcgcgtg ttaacaaaac tgccagcgaa acgccagaac
    19021 ctgctgttct ctgcgacctt ctctgacgat attaaagccc tggcggaaaa actgttgcac
    19081 aacccgctgg aaatcgaagt ggcacgccgc aataccgcgt ctgatcaggt gacccagcac
    19141 gttcactttg ttgataagaa acgcaaacgc gaattgctgt cgcacatgat tggtaaaggg
    19201 aactggcagc aggtgctggt gtttacccgt accaaacacg gcgctaacca tctggctgag
    19261 cagctcaata aagatggcat ccgtagtgcg gcgatccacg ggaataaatc gcaaggtgcg
    19321 cgtacccgtg cgctggctga ttttaaatcg ggtgacattc gcgtactggt ggcgaccgat
    19381 atcgctgcgc gcggtctgga tatcgaagag ttgccgcacg tcgtcaacta tgaactgcca
    19441 aacgtaccgg aagattatgt ccaccgtatc gggcgtaccg gtcgtgcggc tgctaccggt
    19501 gaagcgttgt cgctggtgtg tgttgatgaa cacaaactgc tgcgtgatat cgaaaaactg
    19561 ctgaaaaaag agatcccgcg cattgcgatt ccgggctatg agccggaccc gtcaatcaaa
    19621 gccgaaccga tccagaacgg tcgccagcaa cgtggcggcg gcggtcgtgg gcaaggtggt
    19681 ggtcgcggtc aacagcaacc acgccgtggg gaaggtggcg caaaatctgc aagcgcgaaa
    19741 cctgcagaaa aaccgtctcg ccgcctcggc gatgccaaac cggcaggcga acaacaacgt
    19801 cgccgccgtc cgcgtaaacc taccgctgcg cagtaatctt ttatgccggg ctatgcccgg
    19861 catcaggctg atgaacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    19921 aactctgcaa tatattgaat ttgcatgctt ttgtaggccg gataaggcgt ttacgccgca
    19981 tccggcattt cacaacaagc acttgtcagc aatttgagaa cacgggaaaa aatttatctg
    20041 ttttaccgcc cataagccac cgagacgtta cttctctata gccaattgtt cgcgcaactc
    20101 cattaaaagg tagcccagtc gattcttgcc cttaccatta ccaccgtctc cccagtaagc
    20161 atcgttttct gtatgctcaa ccagttttgc gggcgcggtt gccagcaaga gcgcacgcag
    20221 ttctggatgc tgttcgaatt tagcgcgaag tgcttttcgc atcacctgtt ctttgaccga
    20281 ctcccagttt ttacgcagag gcttagaacg atcgcgcccc atgcgtgcgg cgaccatagg
    20341 ggaagaaacc cgacggatct cttcgcggta tttttcatca aggaattttt gtgcctgaaa
    20401 atagtgttct gaggtgggcc aggttttccc gtcaaccttg atgggccagg cggcaaagtt
    20461 agaaaaatca ccgtagtcat cgctggtgct gtagaaattt atgatggtgt cttgcatgac
    20521 gtgttggatc ctttgtgctc gaacgggcat taaaccgcat tatgttggtg gttattgcga
    20581 gccgctttcc agaaacagaa aaaccattac ccctgaaaac cgaaaaatgc cacaatattg
    20641 gctgtttata cagtatttca ggttttctca tggcattaac cgccgcgctt aaagcgcaaa
    20701 ttgccgcctg gtataaggcg cttcaggaac agatccccga ctttattccc cgtgcgccgc
    20761 agcggcagat gattgcggac gtcgccaaaa cgctggccgg agaagaaggg cggcatctgg
    20821 cgattgaagc ccccaccggc gttgggaaaa cgctctccta tttgatcccc ggcatcgcca
    20881 ttgcccgcga agagcaaaaa acgctggtgg tgagtaccgc caacgtggcg ttgcaggatc
    20941 agatttacag caaagattta ccgctgctga aaaagatcat tcccgatctt aaattcactg
    21001 ccgcttttgg gcgtgggcgc tacgtttgcc cgcgtaatct gacggcgctc gccagcactg
    21061 agcccacgca acaggatctg ctggcgtttc ttgacgacga actgacgcca aataatcagg
    21121 aagagcaaaa acgttgtgcg aagctgaagg gcgatctcga cacttataaa tgggatggtc
    21181 tgcgcgatca tactgatatc gctatagatg acgatctctg gcgtcgttta agtaccgaca
    21241 aagccagctg cctcaaccgc aactgttact actatcgtga atgcccgttt tttgttgctc
    21301 gtcgggagat tcaggaagcg gaagtggtgg tggcaaacca tgcgctggtg atggcggcga
    21361 tggaaagcga agccgtacta cctgacccga aaaatctgct gctggtgctg gacgaaggtc
    21421 atcacctgcc agatgtggcc cgggatgcgc tggagatgag tgccgaaatc accgcgccct
    21481 ggtatcgact acagctggac ttgttcacga aactggtcgc tacctgcatg gagcagtttc
    21541 gcccgaagac catcccaccg ctggcgatcc ctgaacgttt gaatgcgcat tgtgaagagt
    21601 tgtatgagct tatcgcctcg ttaaacaaca ttctcaatct ctacatgcct gccgggcagg
    21661 aggcagagca ccgttttgcg atgggcgaac tgcctgatga agtgctggag atctgccagc
    21721 ggctggcaaa actcaccgag atgctgcgtg gtctggcgga gttatttctt aacgatttaa
    21781 gtgagaaaac cggcagccac gatattgtgc gtctgcatcg gttgattttg cagatgaacc
    21841 gcgcgttggg gatgttcgag gcgcaaagca aactctggcg gctggcgtct ctggcgcaat
    21901 cttccggtgc gccggtgacc aaatgggcga cgcgggaaga gcgcgaaggg cagctgcatc
    21961 tctggtttca ctgcgtgggg atacgtgtca gcgatcagct ggaaaggctg ctgtggcgca
    22021 gtattccgca cattattgtt acctccgcaa ccttgcgttc gctgaatagt ttttcgcgtt
    22081 tgcaggagat gagtggtctg aaagagaaag cgggcgaccg ttttgtggcg ctggattccc
    22141 cctttaacca ctgcgaacag ggcaaaattg ttattccccg gatgcgcgtt gagccttcca
    22201 tcgacaacga agagcagcat attgccgaaa tggcggcctt tttccgtaag caggtggaga
    22261 gcaaaaaaca tctcggtatg ttggtactgt ttgccagcgg acgggcgatg cagcgctttc
    22321 tcgactatgt gacggattta cgcctgatgc tgctggtgca gggcgatcag ccgcgttatc
    22381 gcttagttga actgcaccgc aaacgcgttg ccaacggtga gcgtagtgtg ctggtgggct
    22441 tacagtcatt tgccgaaggg cttgatttga aaggtgatct gctcagccag gtgcatatcc
    22501 acaaaatcgc ttttccgccc atcgacagcc cggtggtgat caccgaaggg gaatggctga
    22561 aaagcctcaa ccgctatccg tttgaggtgc aaagcctgcc gagcgcctcg tttaacctga
    22621 ttcagcaggt tgggcgactg attcgaagcc acggttgctg gggcgaagtg gttatctacg
    22681 acaaacgctt gctgaccaaa aattatggca agcgactact ggatgcatta ccggtatttc
    22741 cgatagagca accggaagtc cctgaaggta tagttaaaaa gaaagaaaaa acgaaatccc
    22801 cacgccgtcg gcggcgttaa tgatgtgagt caggtaagga gtcgtaaatg gactatcgca
    22861 aaatcattaa agagatcggg cgcgggaaaa accacgcgcg cgatttagac cgggatactg
    22921 cgcgcggtct gtatgcccat atgctcaatg gtgaagtccc tgaccttgag ttgggcggcg
    22981 tattgattgc gctacgtatc aaaggggaag gggaagcaga gatgctcggc ttttacgaag
    23041 ccatgcaaaa tcacaccatc aagctgacgc cgccagcagg caagccgatg ccgattgtca
    23101 tccccagtta caacggcgcg cgtaaacagg ccaacctgac gccgttgttg gcgattctcc
    23161 tgcacaaact cggttttcct gtggtggttc acggggttag cgaagatcca acccgcgtgc
    23221 tgactgaaac catttttgaa ttgatgggta ttacgccaac gttgcacggc gggcaggcgc
    23281 aggcgaagct cgacgaacat caaccggtgt ttatgccagt cggcgcgttt tgcccgccgc
    23341 tggaaaaaca actggcgatg cgctggcgga tgggcgtgcg taacagtgca cataccctgg
    23401 cgaaactggc gacgccattt gccgaaggtg aggcgctgcg tctttccagc gtttcgcatc
    23461 cggaatacat tggacgcgtc gcgaagttct ttagcgatat cggcgggcgg gcgctactaa
    23521 tgcatggtac tgaaggtgaa gtgtatgcta atccgcagcg ctgcccgcag atcaatctca
    23581 ttgaccgtga agggatgcgg gtgctgtatg aaaaacagga ccctgctggt agcgagttac
    23641 tgccacaagc aaaagatccg gaaaccacgg cgcagtggat tgagcgttgc cttgctggca
    23701 gcgaaccgat tcccgaatcg ctgaaaatcc agatggcttg ctgcctggtg gctacgggtg
    23761 aagcggcaac tatcagcgac ggcctggcgc gcgttaatca ggcattttaa ttcttttctc
    23821 agccggatga gccatgtcat ccggctttta ccccaccgca atatgaaatt cctgcatctt
    23881 tattgacctt cccgcgcccg gcgtgcagca taaaaaaaca acaaacacat aacataaaca
    23941 ggagttaacc atggaaagtg gtcatcgctt tgatgctcag acgctgcaca gttttattca
    24001 ggctgtattt cgtcagatgg gtagcgagga acaagaagcg aaattagtgg ccgatcattt
    24061 aatcgcggca aacctggcag ggcatgattc tcatggtatt ggcatgatcc caagctatgt
    24121 gcgttcctgg agtcaggggc atctgcaaat taaccatcat gccaaaatcg ttaaagaggc
    24181 gggggcggca gtcacgctcg atggcgatcg cgcattcggt caggtcgcgg cacacgaagc
    24241 gatggcgctg gggattgaga aagcgcatca gcacggtatt gctgccgtgg cgctacataa
    24301 ctcgcatcat atcggccgta tcggttactg ggcggagcag tgtgcagcgg cggggtttgt
    24361 ctctatccac tttgttagcg tggtcggtat tccaatggtc gcaccgttcc acggtcgcga
    24421 cagccgcttt ggcaccaatc cgttctgtgt ggttttccct cgtaaagata atttcccgct
    24481 gttgcttgat tacgccacca gcgccattgc atttggcaaa acccgcgtcg cctggcataa
    24541 aggcgtcccc gtgccgccag gttgcctgat tgacgttaac ggcgtgccga cgaccaatcc
    24601 ggcggtaatg caggagtcgc cgttgggttc gctgttgacc tttgccgaac ataaaggcta
    24661 cgcccttgca gcgatgtgtg aaattcttgg cggggcgctt tccggcggta aaacgacgca
    24721 tcaggaaacg ttacaaacca gtcccgatgc cattcttaac tgcatgacca ctatcatcat
    24781 caacccggaa ctcttcggcg cgccggattg tagcgcgcag accgaagcct ttgccgagtg
    24841 ggtgaaagcc tcgccgcatg atgatgataa gccgattttg ctaccgggcg agtgggaagt
    24901 gaacacgcgt cgcgaacggc aggagcaggg gattccattg gatgcgggaa gctggcaggc
    24961 catttgtgat gcggcgcggc agattggtat gtcggaagag acgttgcagg ctttctgtca
    25021 gcagttagcc agctaaaaaa aagcccgtcc agtggcggac gggcaaacaa gggtaacata
    25081 ggatcaatga gggttagagc atatgcgtct gtcggcaaac agacagggaa atacttgtgc
    25141 tggacgtagc gtaaacgcct gatccgtcca acggttcgga ttttgtaggc ctgataagac
    25201 gcggtagcgt cgcatcaggc atctgttgcc ggatgcggcg taaacgcctt atccttccta
    25261 cgaattactt ataaattacc gcagtaccgc ttaatttatt gttgttggtg gcggaagtga
    25321 tgctgtatcc actggcaccg gctgccgcgg ctttctcagc cagtttcgct tccagggcat
    25381 cgagggtgga tgcgccatcg gcagaaacca cgccgatttt attcatgttc tgtgcctggg
    25441 atgccgttac cggttccgcg gcaaacacgc caaatgacag agttgaaaga gccatagcag
    25501 caacaacagt attgatagtt ttcataatta atctctcgca ggtgattttt gtataagtga
    25561 cgttgtttcg tcgatgtgat gagtatcacg tttttttacg agagataaaa tcgaagagaa
    25621 ttgaccgcct tgttcaaata aattgattga tatctaacat actgataaac catttatttg
    25681 ttgtacttat ggacttgttt tatgcagcgt ggcagtcacg acagcgttaa aggcacggaa
    25741 tgccacgcaa aatacaaatt atactcgcta tacatctcgc cagttgtagt taacgtaact
    25801 ggctgtcttt cgaacctctg cgattatatc ctgtatacgc tggtttttgt aaatctttct
    25861 cctgctgaca atgaatgcat aagcgcacgc caggaatggc ttcccgacgg gcttgcggga
    25921 tgggggcacc gcactcttca cattcatcca ggctttcgcc acgcggaatt tcaccccgag
    25981 cgcgggcaat cgcatcttca attgtactgt tgatctgttc gttgacggcg tcatcgttag
    26041 cccaaccgga tgccatatcg tcctccccat atcaatgctt gtacagttaa gtgtagctaa
    26101 tccagggacg aacttgggca gttcaagcat cagatctccg accattcccg cagcagatta
    26161 tgataaagat taagcagcga caggatctct tcattttcac catagcggct tttcagcgac
    26221 tgaatattgt tgtccagttc aaacagcatg gcgcgctttt tatcatcacg gatcatcgac
    26281 tggatccaca taaatgatgc cactcgtacg ccgcgggtta cgggtgtcac gcaatgcagg
    26341 ctgctggagg gatacaacac gagatcgcct gccgggagtt ttacccgatg ttgtccgaag
    26401 gtgtcattaa cgaccagttc gccgccgtcg tagctttgtg gatcgcttaa aaacagcgtg
    26461 gcagaaaggt cagtacgcat ccagccgttt tgtggatggc tgcgtactgc gccatccaca
    26521 tggaaaccat aggtttcatt gttctgatag cgattaaaca gcggcgtgga aagggtacgc
    26581 ggcaaggccg cggcaaagaa taaagcatgt tggttaaccg cgttcagcac ctcattttgc
    26641 agggcggcgt ataacgtgct gcgggtgtcg acctgttgat tgttcttaac ttgcgcacct
    26701 tgtgcgccgg tggtgacgcg tccatccacc cattcggctt gttccagttg ttcgcgaaac
    26761 cgggcgacgt cctgtggcga taacacgccg ggaatgtggt acatcataca agttctccaa
    26821 aaagtggggc ctgcgcccca catctgaatc agaaatgcat attggctgtg agcaagaagg
    26881 ttcttggctc gcccgggtga taacggtagc cgctcttgtt gattgaggcg acgtaatcgg
    26941 tatcaaacag gttgtaaacg tttagctgga agtcgagatt gcgattaacc cgatacccca
    27001 gtttggcatc ggcgacccag taaccttcgg taaacgctgg cgttcccacc gcgccgtctg
    27061 aacctttatg catactgccg atatagcgtg cgcccgcgcc aacagagata tcatctgttg
    27121 cctgatactg gctccataag gtgaaggcgt gctccggggt atacggcagc gatgaggaac
    27181 catcctgggc aacatctttg ccgtttttga tggttgcttt ttgctgggta tagccgccaa
    27241 tcacctgcca cgcgggcgtg atattcccgg ccacggatat ctcatagcct tcgacacgtt
    27301 tcttaccgta ttgcgagtaa gttccgtcat cattttgctc aacttcattt tcgatatcag
    27361 tacggaatag cgcggcggtg agcaacaggc gtttatccag aacctgccat ttggtgccaa
    27421 tctcgctggt gttggctttt tgcggtttaa aatcggtgcg gttggcactg ttaccgctgc
    27481 cagactgcgc aagggcgaag ttgttgccgc ccggaggctg ctgggaaacg gcatagttaa
    27541 tatagacatt accgttttcc gtcaggtgat acagcgcccc ggctttccag ttcaccagat
    27601 tgcccgactt ggcggtgtcg acggtggtga ccggagaacc ttttgccaca ccagctgggc
    27661 aggtgatggc accgcgtccg ctgccgccgc aggcggtggc actgtcatat tcagtatgat
    27721 aattatccag acggatcccg ccgttcagct caaaatcacg ggtgatttgc agcgtatcga
    27781 aggcgtaaat tgcgaaggta tccgtctgac cattggcgtt tgcgccgttg cgcgtcaggc
    27841 cgccgggatg aatgctgctg tcaggatgat aaatatttac cgcgggtaac gtcaccggat
    27901 taacgccgta gttagtctgc gtttcacggg taaattccac gccggtactg acatcatgac
    27961 cgatagaacc ggtatagaac gtcgaggtca ggttggtctg gttggtgaga attttattac
    28021 tcacatcttt ggtattcgcc gtgcgtgacc aggtccagct attcacatcg ctggtgggct
    28081 gagtaatatt cgacgccccg cccataatcg ccgtcatcag gtaatcctgc tttacgcgcg
    28141 accaacgggt agtattgcga atggtggtgt tatcgttgat gtcgtgctca aaacgcatgg
    28201 tggcggtgtc ggtggtcgaa tcgtcgtaat cggaatccgt gccgtaaaag ttatgagtat
    28261 caacttttcc ggaatgattc agagccgctg ttcccgcaga tggggcagaa taacccggca
    28321 aaccgatggt cggaatgccg ccgtctggcg tgttgtgctg ggtgacatgc agataattaa
    28381 gatacaaacg attcgctgta ccaaggccaa aagcgataga aggggcgacg ccgtaacgct
    28441 catttttgac tttgtcgcgt ccggcatcgt gcgttttttc gcccatcaca ttcaggcgca
    28501 ccgcagtggt atcaccaatg acctgattga cgtccagcgt gccgcggcgg aaccaggcgc
    28561 tgccaatact ggcggaggcg tcaatgccgg aatcattgcg cggctgcttg ctgatcatat
    28621 tgatcgagcc cgtcggcgcg ctgcgcccgt agtcggtgcc ggaaggccct ttaatcactt
    28681 cgacctgctc agtattgaag gtgtcgcgcg agacgctgcc gatatcgcga atgccatcaa
    28741 tataaatact gttagaggta tcagcaccgc gcatataaat ggcgtcgcca gtggtggagt
    28801 taccgttctc acccgcaaaa aacgcaccca cgccggggac gtttttgagc gcgtcggtaa
    28861 ggttggttgc gccctgatct ttaatcactt gttcagaaat caccgtcatc gtgcgggtag
    28921 tatccgctac cggacgcgag aatttcggat cggcagattg ttgtggcgca taaagcgaag
    28981 gcgttgatgc ttcgacaacc agcgtgtcat ccgcgttagt ttgcccttcg gcggcgagtg
    29041 cctgagccac aggcgtgatg ccaatacaaa gaccggcaaa gaacgtgagc gaatgaaatt
    29101 gtctggcagg gaaattgcga ttgttttcca ttttgcaggt gacttttttt atatgttgaa
    29161 attttcactg ccacgagaaa gcctctctgc ggatgcgatg ccactgttaa tctcgtgata
    29221 gtgttcgtgc aagtgataat aattttgata agcattatca cttggcgcga ttttctatca
    29281 cccattgcgc gaaaaagaaa tacatttatt tacattgcgt tcatatttga tgttttttga
    29341 ttacaaaaca ggcgaaaact gcatccagag gcgaatgcag ttacgttggt ggggttattt
    29401 gtagatggtt gcagtaccga acatctggtc atttccgcct gcggaattaa tgacataacc
    29461 tttcgcgcct tgctcgcgcg ctttttctgc cagtttatcc tccagatcgc ttaggtttga
    29521 tgcgccagtt gccgaaacgg taccggcggg ccgtagctga ccgctgtcca ggttactcat
    29581 cggttgcgcg gcataagccg tgagagagat tccgctcagg acggtggcaa tcagtagtgt
    29641 gaggcacttt ttcataatga catcctcata aatacaacgg gtagtgcctg ataagtgtag
    29701 gtcgcctggg cgatgaaaat gacgcaaaaa attgacagag atgtgcttat tttttgaacg
    29761 atatattttt acaaaataag acaaatcaat gacatgcata atgctgattt ttatgatgaa
    29821 ttgccataaa ccatagccat gttaaggttt tggggaatga taaacgcagc cagatattta
    29881 ccctgtcgcg ttatgcgggt atcttacgcc gctgcttaaa ggagaatgct atgtccgccc
    29941 agaaaccggg gttgcatccg cgcaaccgtc atcacagccg ctacgatctc gccacgcttt
    30001 gtcaggtcaa tcctgaactc aggcaattcc tcacgcttac acccgcaggg gagcaaagcg
    30061 tagactttgc caatccgctg gcggtgaagg cgctcaataa ggcattgctg gcccattttt
    30121 acgccgtagc gaactgggat atcccggacg gttttctctg cccgccagta ccgggccggg
    30181 cggattatat tcatcacctt gccgatttac tggcagaagc aagcggaaca attccagcta
    30241 atgccagcat tctggatatc ggcgttggtg cgaactgtat ttatccgctg attggcgtac
    30301 atgaatatgg ctggcgtttt accggtagtg aaaccagcag tcaggcgtta agtagtgcgc
    30361 aggcgatcat cagtgctaat ccggggctta accgcgccat tcgtctgcgt cggcaaaaag
    30421 agagtggggc gatttttaac ggcatcatcc ataaaaacga gcaatacgac gcgaccttgt
    30481 gtaacccgcc attccacgat tccgccgctg cggcacgggc aggtagtgag cgtaaacgcc
    30541 gtaacctggg gctgaacaaa gacgatgcac tgaactttgg cggccagcaa caggagttgt
    30601 ggtgtgaagg tggtgaagtc gcctttatca aaaagatgat tgaagagagc aaaggcttcg
    30661 cgaagcaggt gatgtggttt acctcgctgg tttctcgtgg tgaaaactta ccgccgttgt
    30721 atcgtgctct gacggacgtg ggcgcggtga aggtggttaa aaaagagatg gcccaggggc
    30781 aaaagcagag tcgctttatt gcctggacct ttatgaacga cgagcagcgc cgccgttttg
    30841 ttaatcgcca acgttaaagc gttggctccc ccggcggcag cggttcagcc ggggccgcgc
    30901 ccggagcagg cagcacctga taagtctgca ctggcgcgcg aacacctgcc aggtcgaaat
    30961 gctttttcac ctggctgtcg agggcaaagc ggaccgtcca ctgtttgagt ggcagcgtgg
    31021 tgaacgaaac acgcagtgta aacgcggtat tgcttaagcc gacaatcccg gcaaagtttg
    31081 gttcaccaat aaccagcccg cgaatttctt cgttttccat caattccgct accgcatctt
    31141 tcagtgcctg attggcttta tcagcatctt catggcgatc aacatcgtag tttgccacca
    31201 ccgaaccaat gccgcggacg aagttagcaa aggtggttat cgaagaccaa ggaatgatgt
    31261 gatacgcccc ggtatcctga cgcacgccca cggagcgaat cgacatccgt tccacagtgc
    31321 cggtcaacgg cccgatagta accaaatctc cagtgttcat gccgttttca aactgaataa
    31381 ataccccggt gataatatct ttcaccagcg tttgcgaacc aaacgagata gccagaccta
    31441 atgccccagc acctgccagc aatggcgcga tattgacgcc gatttccgac aacacaatca
    31501 tgatggtgat ggtactgata atcaccgcca gcgcgttacg gaacagcgtc agcagggtac
    31561 gcgtacgggc gctgggtagc gggcggccat gaatatccga agccagccgg ttttcgatca
    31621 aactggcgag cactgtccag ccaaccgccg agaagaaaag aatgagtgcg atgcggatca
    31681 ggatatctac ggttttctgc cccgcgccgt tttgcagcca gttccagaaa tcgaacaatc
    31741 cccatgcgct caacagcaac attaccgcca cgcagacggt cagaatacgc gccgttttca
    31801 gcgccgccga aagccagcca ttcagccgtt tttgtagctc cggatagtta cgctgagtat
    31861 gtggcgagag ggtgatggtt ttcgccagcc agcgggaaaa cataccggaa acaaacgctg
    31921 cgataccaat aatcgccagg ctgcgcaccg ttgcacccat cataaatttc aggctgttgc
    31981 ccggatcgaa caacgaaaag aaaaacagca cgataaaata ggcgcttgcc agccagtgcc
    32041 acaccagcgc aaaggcgcgg ataaacaggc tgaaaaaggc cagcgaatgc tccgcaaagt
    32101 tgagcaaatg ttgggtaatc tcttttttat tgcgaaagat caggtacaac gcccagacgg
    32161 tcatgcacag cataatgatg acgttcgcca gcgcacctat ctgtacattc acctgattag
    32221 agataatcgg cacggccaca atcaggccat aacctatcaa actgcttaac cagctcaggc
    32281 gacgactcca gtaacgggcg ctctcgtcct gaatcgtgaa cgggcgcagt tcagccacgt
    32341 ttgggcaaaa aatcaggcgt agtacggctt tgaaaaattc aatcagggca aaggcgttga
    32401 gaaacaaact ttgttggaaa gcgatggtgc gactgcccgc attcaggttg tcgcttaata
    32461 cctggccgac aaacaatgtc agtgccagta acagtaggtc gataataaac gccccgataa
    32521 tcatcgccgg aagctgcagc cagttgctgc gttcgcgatt tttttgccgc gcccactggc
    32581 ccattttgcg atacagcggc agtgcgcaca gacgaatcag ccagtaaaaa ccaaacacta
    32641 ataccgctaa cattgaaaaa tgggtcaacg cattgctgaa ggtttgtgga ttaaacggct
    32701 tatgcgggga gccggtgata ttgcgataaa gttgtccgaa gcgggcggaa agggcttcac
    32761 cataatggcg gctgacttcg gtgacctttt gcagcacggt ttgctcttca accagcgtcg
    32821 gcggcacgat cttcgggacc ggttccgcag ggggcgtcgc ggcgacagta cgcaactggt
    32881 cgatcaattc tttacgcgag gtgtcgttat ccagcacatc cgccagtgcg ccataggccg
    32941 cttttttttg ttcgatatcc ggttccgggg ccggttcagt cgttgagtcc gttgtcgttg
    33001 tggttgtaac gccgggtatg gataccgcgt gggcaggtgc gcccagcagg cagaagagga
    33061 tgaacaggat ccaccgcatg actcctccag tgagaaaaca gagcaaaaag ataagtatag
    33121 atgctggggg aggggcgatt ttaaatgaga ggaatctggt gtgcctccct ttcgggtgaa
    33181 agggaggcag agaagttaag agacgtgctg caaaaattcc tgcaagcgct ggctcggcgg
    33241 gttcttgatc aatacctgcg gatcgccatc ttccgcaatc cggcctttgt cgataaagat
    33301 cagccgcgaa gctactttct cggcaaaacc gatttcgtgg gtcacgatca ccatcgtcat
    33361 cccttcttca gccagatcct gcataacctt cagcacttca tggcgcagtt ccgggtcaag
    33421 agcggaagtc ggttcatcaa acagcatcat tttcggcttc accgccagcg cgcgggcaat
    33481 cgccacacgc tgctgttgac caccagaaag ttcggaaggg tagtgatgtg cacgttctgc
    33541 cagaccgact ttcgccagca gctcacgcgc cagtttttcc gcctcttctt tgttcgcgcc
    33601 acgcacgcgt agcggaccaa acatgacgtt ttccagcgcc gtcagatgcg ggaagaggta
    33661 aaactgctgg aacaccatac ctgcttcctg gcgaatcagg cgctcgtcaa ctttcggatc
    33721 gttaaccttc aggccatcga caatcagatc gccggaggtg atttcttcca gtttgttgat
    33781 gcagcgcagc agggtcgatt taccggaacc ggacggcccg ataatcacca cgacttcgcc
    33841 ctgggcaatg ttcaaatcga tattgtgcag cacctgggtt gggccaaagt gcttggagac
    33901 gtttttaaat tcaatcacag gattttcatc cttctttcca gacgacgcag aataaagctc
    33961 agcaccaggg taataatcag atagaacacc gccacggcgc tccagatctc aagggcgcgg
    34021 aagttaccgg caataatttc ttgcccctga cgggtcagtt ccgccacgcc gatcacaata
    34081 aacagcgagg tgtctttaat gctgatgatc cactggttac ccagcggcgg cagcatacga
    34141 cgcagtgcca gcggtaaaat gacgtagcga atggtttccc aacgtgaaag accgagcgcc
    34201 agtcctgctt cacgaaaacc tttgtggata gacagcaccg caccacgcgt gatttccgca
    34261 atgtacgcgc cggagttgat catgatggtg accaccgccg cagtaaatgg gtcgatgcgt
    34321 aagtcgttaa acgccatcgg cagggcgaaa tagataaaca tcacctggac gacgataggt
    34381 gtgccgcgga tcacttcaat aaagaccagc gcgacgtggt tggctatcca accaccgaag
    34441 gtgcgtgcaa aacctgccag caatccgatt accagaccgc ctgccagacc gaggaccgaa
    34501 atccacaggg tcattttggc accttcaatc agaagcggaa tggcaggcca gatggcactc
    34561 cagtcaaact gcatatgttg ttcctgttac cgtggtgtaa ataatcaaat tcagggtaat
    34621 taatggcccc ggagcgggtt tgcgcccctc accctaatcc tctccccata ggggagaggg
    34681 aactgccagt gcgttttaca ggtgtagcgt tattatttcg gttcagtacc gaaccatttt
    34741 ttgtagattt cgttgtaagt tccgttctcg cgcagggttt tcaacgcgcc gttgactttg
    34801 tcacgcagct cgtcgctacc tttcgggaac gcaataccgt attgctgcgc ttccagagag
    34861 tcacctaccg ctttgaactg accgttaccg gcggttttga tgaagtacag aatgtttggc
    34921 gtatcgtgca gaacggcgtc tgcgcggttg gtgcccagtt ccatataggc gttatcgatg
    34981 ttcgggaact gacgcagatc tttagttttg atgtttgctt tcgcgtaatc aacggagcca
    35041 gtaccgctct tcacagcaac cactttcccg tcgagatctt tcacgctttt cacatcgtta
    35101 ttgttagctt tcaccatcac taacaggccg cttttgtagt agccgtcaga gaaatcgatc
    35161 gcttttttac gctcgtcggt gatggtaatg cccgccagcg ccagatcgac gtttttggtt
    35221 tgcagtgccg gaatgatccc actgaaatcc atcggcttca gttcgtaatc cagcttcagc
    35281 tctttagcga tggcagccca cagatcaacg tcaaagccca catatttatc gccctgttta
    35341 aattcaaacg gaacgaaggc ggtatccgtc gcgacaacta attttttatc cgcggcatga
    35401 gaagaaaccg caaaagccag ggtcagtgca gccagtgaaa cttttaatac agacttcata
    35461 gcatttcctt ttttctatcc acgggacgat cccctgcgtg acattcacat atatgaaaaa
    35521 atcgtgccag ttttgcaact ccttgatttt tcgagatgcg gcaaaaacgt gatgcacgat
    35581 ttatagggca ataccctgaa gatgcaccat tctggggcac caatctggtg cgctaaaatt
    35641 gtgcactcaa cacagtgatt atttagcgta aaacctgatg aaaaaacaat ctttatgtaa
    35701 cgattgtgtg atgatgtgga tacaaaaaat ttacttaatc agctggagat agcagatgga
    35761 tgcactaaat aagtgcgttg aggtgggctg caaaacaaaa cggcctcctg tcaggaagcc
    35821 gcttttatcg ggtactaaag ttctgcacca tcagcgatgg atttattcga tgttagactc
    35881 gataaaccac aggaatttat ccaggtcgcg agacgcggcg gtcaggatat ctgcggtgtc
    35941 gtcatctttc gcttcgccaa tcgctttgcg tacgtcatta gcgacgattg cgtaacggtc
    36001 agccagttct ttcaggtgat cctgaacgtt gtggatgtcc agcgggtaac ttttcagcgg
    36061 ggttttgctg ttgataactt gagtggtccc cagagctaca ccgcccagct gcactgcacg
    36121 ttctgccatg gtatccagat gatcgatcag tgcggtgcgg aagccatcca gcatttcatg
    36181 tacggcaatg aagttagcgc cgcgcatgtt ccagtgcgct tgtttggtaa tcaaagaaag
    36241 atcaataaac tggataacct ggcgattcag caactctact gttgcttttt tctcgctgtc
    36301 ggagacatcg ttgcgggtat aaagcagatt ggtcgctttt gatttaacta atttagcggt
    36361 actcataatt tcatatcctc ttgatgttat gtcccagtaa ttaacgagac taagtatagc
    36421 accggctatg tgttccgcta ttctggctgt tcctatcaca ctaatagtgg taacaagcgt
    36481 gaaaaacaaa actaataaag attcaatgag ttagatatat tgataagaac aattcttatt
    36541 tatctgctcg cc
//
LOCUS       DAZFWP020000032        36460 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFWP020000032 DAZFWP020000000
VERSION     DAZFWP020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.032"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            173..1390
                     /gene="alaA"
                     /locus_tag="ACHK0Z_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             173..1390
                     /gene="alaA"
                     /locus_tag="ACHK0Z_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU4501495.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1474..2073
                     /gene="yfbR"
                     /locus_tag="ACHK0Z_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             1474..2073
                     /gene="yfbR"
                     /locus_tag="ACHK0Z_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4501496.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2132..3964)
                     /locus_tag="ACHK0Z_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             complement(2132..3964)
                     /locus_tag="ACHK0Z_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4501497.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4051..4701)
                     /gene="hxpA"
                     /locus_tag="ACHK0Z_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     CDS             complement(4051..4701)
                     /gene="hxpA"
                     /locus_tag="ACHK0Z_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU4501498.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4712..5206)
                     /locus_tag="ACHK0Z_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     CDS             complement(4712..5206)
                     /locus_tag="ACHK0Z_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU4501499.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5289..5744)
                     /gene="yfbV"
                     /locus_tag="ACHK0Z_RS07260"
                     /old_locus_tag="pgaptmp_001452"
     CDS             complement(5289..5744)
                     /gene="yfbV"
                     /locus_tag="ACHK0Z_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU4501500.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6082..7284
                     /gene="ackA"
                     /locus_tag="ACHK0Z_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             6082..7284
                     /gene="ackA"
                     /locus_tag="ACHK0Z_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU4501501.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7359..9503
                     /gene="pta"
                     /locus_tag="ACHK0Z_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             7359..9503
                     /gene="pta"
                     /locus_tag="ACHK0Z_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4501502.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9693..11213
                     /gene="yfcC"
                     /locus_tag="ACHK0Z_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             9693..11213
                     /gene="yfcC"
                     /locus_tag="ACHK0Z_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU4501503.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11246..11788)
                     /gene="yfcD"
                     /locus_tag="ACHK0Z_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             complement(11246..11788)
                     /gene="yfcD"
                     /locus_tag="ACHK0Z_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU4501504.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11846..12397)
                     /gene="yfcE"
                     /locus_tag="ACHK0Z_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             complement(11846..12397)
                     /gene="yfcE"
                     /locus_tag="ACHK0Z_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU4501505.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12453..13097)
                     /gene="yfcF"
                     /locus_tag="ACHK0Z_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(12453..13097)
                     /gene="yfcF"
                     /locus_tag="ACHK0Z_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU4501506.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13233..13880
                     /gene="yfcG"
                     /locus_tag="ACHK0Z_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             13233..13880
                     /gene="yfcG"
                     /locus_tag="ACHK0Z_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU4501507.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13937..14299
                     /gene="folX"
                     /locus_tag="ACHK0Z_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             13937..14299
                     /gene="folX"
                     /locus_tag="ACHK0Z_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU4501508.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14320..15213
                     /locus_tag="ACHK0Z_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             14320..15213
                     /locus_tag="ACHK0Z_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU4501509.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15261..16151)
                     /gene="rpnB"
                     /locus_tag="ACHK0Z_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             complement(15261..16151)
                     /gene="rpnB"
                     /locus_tag="ACHK0Z_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU4501510.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIAHKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16348..17121)
                     /gene="hisP"
                     /locus_tag="ACHK0Z_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             complement(16348..17121)
                     /gene="hisP"
                     /locus_tag="ACHK0Z_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU4501511.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17129..17845)
                     /locus_tag="ACHK0Z_RS07320"
                     /old_locus_tag="pgaptmp_001464"
     CDS             complement(17129..17845)
                     /locus_tag="ACHK0Z_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4501512.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17842..18528)
                     /gene="hisQ"
                     /locus_tag="ACHK0Z_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             complement(17842..18528)
                     /gene="hisQ"
                     /locus_tag="ACHK0Z_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU4501513.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18618..19400)
                     /gene="hisJ"
                     /locus_tag="ACHK0Z_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             complement(18618..19400)
                     /gene="hisJ"
                     /locus_tag="ACHK0Z_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU4501514.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19621..20403)
                     /gene="argT"
                     /locus_tag="ACHK0Z_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             complement(19621..20403)
                     /gene="argT"
                     /locus_tag="ACHK0Z_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU4501515.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20669..21238)
                     /gene="ubiX"
                     /locus_tag="ACHK0Z_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             complement(20669..21238)
                     /gene="ubiX"
                     /locus_tag="ACHK0Z_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU4501516.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21333..22850)
                     /gene="purF"
                     /locus_tag="ACHK0Z_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             complement(21333..22850)
                     /gene="purF"
                     /locus_tag="ACHK0Z_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU4501517.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22887..23375)
                     /gene="cvpA"
                     /locus_tag="ACHK0Z_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             complement(22887..23375)
                     /gene="cvpA"
                     /locus_tag="ACHK0Z_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU4501518.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23634..24296)
                     /gene="dedD"
                     /locus_tag="ACHK0Z_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             complement(23634..24296)
                     /gene="dedD"
                     /locus_tag="ACHK0Z_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU4501519.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24286..25554)
                     /gene="folC"
                     /locus_tag="ACHK0Z_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             complement(24286..25554)
                     /gene="folC"
                     /locus_tag="ACHK0Z_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU4501520.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25624..26538)
                     /gene="accD"
                     /locus_tag="ACHK0Z_RS07365"
                     /old_locus_tag="pgaptmp_001473"
     CDS             complement(25624..26538)
                     /gene="accD"
                     /locus_tag="ACHK0Z_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU4501521.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26694..27353)
                     /locus_tag="ACHK0Z_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             complement(26694..27353)
                     /locus_tag="ACHK0Z_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4501522.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27436..28248)
                     /gene="truA"
                     /locus_tag="ACHK0Z_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             complement(27436..28248)
                     /gene="truA"
                     /locus_tag="ACHK0Z_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU4501523.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28248..29261)
                     /locus_tag="ACHK0Z_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             complement(28248..29261)
                     /locus_tag="ACHK0Z_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4501524.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29327..30463)
                     /gene="pdxB"
                     /locus_tag="ACHK0Z_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             complement(29327..30463)
                     /gene="pdxB"
                     /locus_tag="ACHK0Z_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU4501525.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30562..31557
                     /gene="flk"
                     /locus_tag="ACHK0Z_RS07390"
                     /old_locus_tag="pgaptmp_001478"
     CDS             30562..31557
                     /gene="flk"
                     /locus_tag="ACHK0Z_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU4501526.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31554..32732)
                     /locus_tag="ACHK0Z_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             complement(31554..32732)
                     /locus_tag="ACHK0Z_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4501527.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33016..34236)
                     /gene="fabB"
                     /locus_tag="ACHK0Z_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             complement(33016..34236)
                     /gene="fabB"
                     /locus_tag="ACHK0Z_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU4501528.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFSADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34395..36401
                     /gene="mnmC"
                     /locus_tag="ACHK0Z_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             34395..36401
                     /gene="mnmC"
                     /locus_tag="ACHK0Z_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU4501529.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT         9007 a         9928 c         9071 g         8454 t
ORIGIN      
        1 atatacacct cattattttg tcattattaa gttattaaca gcacaatcga gccttccact
       61 ctggcaaaat cttattctgc agaccttcaa aacaccgtcc tgggggagta cattgttcta
      121 agctgacttc cacggcaggg agtggcgata acagcaaaaa aggtcaagat tcatgtcccc
      181 cattgaaaaa tccagcaaat tagagaatgt ctgttatgac atccgtggtc cggtgctgaa
      241 agaagcaaaa cgcctggaag aagaaggtaa caaggtactg aaactgaaca tcggcaaccc
      301 agccccgttc ggttttgacg cgccagatga aatcctcgtt gacgtgatac gcaacctgcc
      361 taccgctcaa gggtattgcg attccaaagg gctttactcc gcgcgtaaag ccatcatgca
      421 gcactaccag gctcgtggca tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg
      481 tgtatcggag cttatcgttc aggcaatgca ggcattgctg aacagcgggg acgaaatgtt
      541 ggttcctgca ccagattacc cactatggac cgcggcggtt tcgctttcca gcggtaaagc
      601 ggtgcattat ctttgcgatg aatcctctga ctggttcccg gacctcgatg atattcgcgc
      661 taaaattacg cctcgtacgc gtgggatcgt tattatcaac ccaaataacc caaccggcgc
      721 ggtatattcc aaagagcttt taatggagat tgtggagatt gcacgtcagc ataatctcat
      781 tatcttcgcc gatgaaattt atgacaaaat tctctacgac gacgctgagc atcactcaat
      841 tgcgccgctg gcacctgacc tgctgaccat tacctttaac ggactgtcga aaacgtaccg
      901 cgttgcaggc ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg
      961 ctacatcgaa ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca
     1021 acacgccatt cagaccgcgc tgggtggtta tcagagcatc agtgaattta ttacccctgg
     1081 cggtcgtctt tatgagcagc gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt
     1141 ttcctgcgtg aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt
     1201 taacattcac gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa aagtcctgtt
     1261 ggtgcaaggg acggcattca actggccgtg gccggatcac ttccgcattg tcactctgcc
     1321 gcgtgtcgat gatatcgagc tgtctttgag taagttcgcg cgtttccttt ctggttatca
     1381 tcagctgtaa tcttaatttc actgccggag attgcatccg gcagtgttat cccgccacaa
     1441 tgacctgatg atgtcatcat acgtaaggtc actatgaaac agagccattt ttttgcccat
     1501 ctctcacgcc tgaaactcat taaccgctgg ccgctaatgc gcaacgtgcg gacggaaaat
     1561 gtgtccgaac acagtttgca ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat
     1621 cgtaaatttg gcggtaatgt caacgccgaa cgtatcgctt tactggcgat gtaccacgat
     1681 gcctcagaag tgctcaccgg cgatctccct actccggtga aatacttcaa ttcgcaaatc
     1741 gctcaggagt acaaggctat tgaaaaaatc gctcagcaaa aactggtcga tatggttccg
     1801 gaagagctgc aggatatctt tgcgccgtta attgacgagc atgcatatag cgatgaagaa
     1861 aaatcgttgg tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct ggaagaactc
     1921 gcggccggaa ataatgaatt cttgctggca aaaacgcgac tggaagcgac gcttgaagcg
     1981 cgtcgcagcc aggagatgga ctacttcatg gaagtatttg ttcccagctt ccatctttcg
     2041 ctcgatgaga ttagccagga ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc
     2101 aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc gggatcatca
     2161 ccacacaaac cgccatcacg ataatggtga acggtacccc caacttcaca aagtcactga
     2221 agctgtaatt tcccggacct aaaaccagtg tgttaaccgg tgaagaaacc ggtgtcataa
     2281 aggcggcaga tgctgccatc gccacgacca tcgcgaatgg ataaggcgac acccccatcg
     2341 ttttggcagc agccagcgca atcggagcca tcaacaccgc cgtcgcggta ttagagataa
     2401 acagcccaat aaccgccgac aaaacaaaca aacaccccag catcatatgt ggcccgtaac
     2461 cgccgccaat gtccatcagc cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg
     2521 ccacagcaaa cggcatcatc ccaacgatca aaataatgct cggccagtga atggatttat
     2581 aggcgctttc ggcatcgata cagcggaatt tccccatcag caggcaggcg atgatagcgg
     2641 caacaggatt aggaatttca tcggtcagca ttaacgccac cattagcacc agacagaaaa
     2701 tggcatgggg tgcctggctg tgcgcgggcg atgcttcact cacctcttcc ggtaagttca
     2761 gcgctacgaa gtcgcggccc tgttttgcca gcataccgat cagtttccag ttaccaacaa
     2821 ccaggatgat atcgcccagc agcagaggct catccgccag cgaaccttcc agcgccacgc
     2881 cattgcgctt tagccccacc acattcagtc cgtagcgggt acgaaaacca atttcgcgca
     2941 ccgatttacc aatcagttct gactcaggaa ttaatgaaat ctctgccatg cccacatcaa
     3001 gggcctggtc agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat tgctcactac
     3061 aaaattgccg gagatcgaca tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc
     3121 gaaattcaga aaccccatta acgttcacga taacgcgacg aaaacgccgc cagcgttcaa
     3181 caccgatgac gttagcgcca taacgctcac gtaatttgag atcgtccagc cgttgaccaa
     3241 tcattggcga tccggggcga atagccagac gtcgcgcacg cccggtcagt cgatattcac
     3301 ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc
     3361 ctttcagcat gaaacgcatc actaacatat acaaaatacc cagcaccagc acgaccaggc
     3421 caataggtgt tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc aattcactgt
     3481 tgactaccag gttcggcggc gtcgccacca gcgtcatcat gccgctaatc agcccggcaa
     3541 aacttaacgg catcatcaga cgtgacggcg aagtttgcat acgcatggca acgcttaaca
     3601 ccacgggaat aaagatagcg acaacgccgg ttgaactcat aaacgcgcca agccccgcga
     3661 cggtcagcat caacaaaacc aacattttga tttcactgtt gcccgccact ttgaccagcc
     3721 atgttcccat tacggtggca acaccggtac ggaccaaacc atcgccaata ataaacaagg
     3781 cggcaatcag gacaacgtta ggatcagaaa agccggaaaa tacttcgggg accgtcagcg
     3841 ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac cgcatccata cgcactctgc
     3901 ccgtcgcaaa caagacgatg gcaacggcca gtaatgaaag aacccaaatc aattcaccgt
     3961 tcacaactta tccttgttaa ttgaggggga tgacttgatt ctgccataaa aaagccccga
     4021 cgagacgggg ctaaatcatg atcaggtgtt tcactgaata ataacatcgc catttggctg
     4081 tttggtcaca gtaatttgct ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg
     4141 ggtatctgcc ggagcgttaa ccgcaatgac atggcaaccc gccgccaggc cagaaagcac
     4201 gccagcggga gcatcttcca ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc
     4261 acctaacaga tacgcatcag gttctggttt tccgcgcttc actcgctcag cggttacaaa
     4321 cacctctggt gcgggaagcc cagctatttt atggcgcgct cgcgctaccg gcatggagcc
     4381 agaagtcaca atggcccacg gaatacctgc tttattcaaa tgattgagta aggcgattgc
     4441 ccccggaagc gcggtaatac cttcggtttc cgtggcctcg atgtgctcca gacgcgtaaa
     4501 ctcggcggca atatcagcct cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc
     4561 ctgtttaccg tgaatgaaag ccagcacctc ttccggtgct aacccatgac gtctggccca
     4621 gttgctccac gcccgttcta ccgcaggcag ggaatccacc agcgttccat caagatcaaa
     4681 cagaaaacct ttgcaccgca cacgggcctc ctcaggcgtt gataatttga ttaatttcgt
     4741 tggcgctcaa atggtactga cgcgggcagg catgccacac attaagcatg cgctgatatt
     4801 tttcccacat tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc
     4861 gcccttccac attaaccata aagcggacat aaccgaggta acgtgcttca gtggcggcgt
     4921 caaagccgag gaaggtgaca cgacgttcat cgatggattg ctggtcctgc aaattagacc
     4981 aggaaacatg caacgcatga tacatctcca taatgtcgat gatagtgcgg caggtttctt
     5041 ctttcagctc gccaaactcg cgatccaatt cacgcatctg taatccgtaa ccacgctcaa
     5101 taattgtttg caggcgacgg taacgttcag catttgccgg atcgagcata gtcatcatct
     5161 tgtactgatt agacaaaata agacgttgcg cgttggtcat ttccattgtt gactcctgta
     5221 tcactatact acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca
     5281 ggggtcaatt acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc
     5341 gtgtcagcta atgcctggta atcaggcttg ccttcaacgg gtgccaacac ctgtccagac
     5401 tcctgcaatt taccgcgaac ttcataaaac cagttaagga ttgcaggggg taatggcgtt
     5461 acagaacgct tgcccagcca ccacaatccc tgcatgggta aacttaaggc gaacagcgca
     5521 gtggcaactg ccggcccaag ctgaccgccc agggcaatct gccagcagag agtaaatacg
     5581 gcgatcggcg gcataaaacg gatcgcataa cgcgtcatct tgataacgcg attttcgaca
     5641 aagaccgggg caaggcgttt ttccagcggc cacgtctttg agtaatgctg tccccggcga
     5701 aacaagctaa aaaaattaac agaacgatta tccggcgttg acatgcttca cctcaacttc
     5761 acatataaag attcaaaaat ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca
     5821 ttttgtcttc catacccact atcaggtatc ctttagcagc ctgaaggcct aagtagtaca
     5881 tattcattga gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg
     5941 gcatagactc aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat
     6001 cataaatgtc ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccac
     6061 gtatcaatta taggtacttc catgtcgagt aagttagtac tggttctgaa ctgcggtagt
     6121 tcttcactga aatttgccat catcgatgca gtaaatggtg aagagtacct ttctggttta
     6181 gccgaatgtt tccacctgcc cgaagcacgt atcaaatgga aaatggacgg caataaacag
     6241 gaagcggctt taggtgcagg cgccgctcac agcgaagcgc tcaactttat cgttaatact
     6301 attctggcac aaaaaccaga actgtctgcg cagctgactg ctatcggtca ccgtatcgta
     6361 cacggcggcg aaaagtatac cagctccgta gtgatcgatg agtctgttat tcagggtatc
     6421 aaagatgcag cttcttttgc accgctgcac aacccggctc acctgatcgg tatcgaagaa
     6481 gctctgaaat ctttcccaca gctgaaagac aaaaacgttg ctgtatttga caccgcgttc
     6541 caccagacta tgccggaaga gtcttacctc tacgccctgc cgtacaacct gtacaaagag
     6601 cacggcatcc gtcgttacgg cgcgcacggc accagccact tctatgtaac ccaggaagcg
     6661 gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca cctgggcaac
     6721 ggtggttccg tttctgctat ccgcaacggt aaatgcgttg acacctctat gggcctgacc
     6781 ccgctggaag gtctggtcat gggtacccgt tctggtgata tcgatccggc gatcatcttc
     6841 cacctgcacg acaccctggg catgagcgtt gacgcaatca acaaactgct gaccaaagag
     6901 tctggcctgc tgggtctgac cgaagtgacc agcgactgcc gctatgttga agacaactac
     6961 gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac
     7021 atcggtgcct acactgcgct gatggatggt cgtctggacg ctgttgtatt cactggtggt
     7081 atcggtgaaa atgccgcgat ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc
     7141 tttgaagttg atcatgaacg caacctggct gcacgtttcg gcaaatctgg tttcatcaac
     7201 aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa
     7261 gacgcgagcc gcctgactgc ctgatttcac accgccagct cagctggcgg tgctgttttg
     7321 taacccgcca aatcggcggt aacgaaagag gataaaccgt gtcccgtatt attatgctga
     7381 tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg
     7441 aacgcaaagg cgttcgtctg agcgttttca aacctatcgc tcagccgcgt accggtggcg
     7501 atgcgcccga tcagactacg actatcgtgc gtgcgaactc ttccaccacg acggccgctg
     7561 aaccgctgaa aatgagctac gttgaaggtc tgctttccag caatcagaaa gatgtgctga
     7621 tggaagagat cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc gttctggttg
     7681 aaggtctggt cccgacacgt aagcaccagt ttgcccagtc tctgaactac gaaatcgcta
     7741 aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc ccggaacagc
     7801 tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac accaacatca
     7861 ccggcgttat cgttaacaaa ctgaacgcac cggttgatga acagggtcgt actcgcccgg
     7921 atctgtccga gattttcgac gactcttcca aagctaaagt aaacaatgtt gatccggcga
     7981 agctgcaaga atccagcccg ctgccggttc tcggcgctgt gccgtggagc tttgacctga
     8041 tcgcgactcg tgcgatcgat atggctcgcc acctgaatgc gaccatcatc aacgaaggcg
     8101 acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc
     8161 tggagcactt ccgtgccggt tctctgctgg tgacttccgc agaccgtcct gacgtgctgg
     8221 tggccgcttg cctggcagcc atgaacggcg tagaaatcgg tgccctgctg ctgactggcg
     8281 gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct accggcctgc
     8341 cggtatttat ggtgaacacc aacacctggc agacctctct gagcctgcag agcttcaacc
     8401 tggaagttcc ggttgacgat cacgagcgta tcgagaaagt tcaggaatac gttgctaact
     8461 acatcaacgc tgactggatc gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt
     8521 ctccgcctgc gttccgttat cagctgactg aacttgcgcg caaagcgggc aaacgtatcg
     8581 tactgccgga aggtgacgaa ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg
     8641 gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc
     8701 agggtgtaga actgggtgca gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct
     8761 atgttggtcg tctggtcgaa ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg
     8821 aacagttgga agacaacgtg gtgctcggta cgctgatgct ggaacaggat gaagttgatg
     8881 gtctggtttc cggtgctgtt cacactaccg caaacaccat ccgtccgccg ctgcagctga
     8941 tcaaaactgc accgggcagc tccctggtat cttccgtgtt cttcatgctg ctgccggaac
     9001 aggtttacgt ttacggtgac tgtgcgatca acccggatcc gaccgctgaa cagctggcag
     9061 aaatcgcgat tcagtccgct gattccgctg cggccttcgg tatcgaaccg cgcgttgcta
     9121 tgctctccta ctccaccggt acttctggtg caggtagcga cgtagaaaaa gttcgcgaag
     9181 caactcgtct ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg ctgcagtacg
     9241 acgctgcggt aatggctgac gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc
     9301 gcgctaccgt gttcatcttc ccggatctga acaccggtaa caccacctac aaagcggtac
     9361 agcgttctgc cgacctgatc tccatcgggc cgatgctgca gggtatgcgc aagccggtta
     9421 acgacctgtc ccgtggcgca ctggttgacg atatcgtcta caccatcgcg ctgactgcga
     9481 ttcagtctgc acagcagcag taatctcgtc atcatccgca gctttgcgct gcggatatct
     9541 gaaccggaaa taatcactat ttccggtttt ttattctctt aatttgcatt aatcctttct
     9601 gattatcttg cttaactgcg ctgcatcaat gaattgcgcc atctcacttt gcatacttac
     9661 cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa
     9721 caagaagatg ggcaatgccc gatacgttgg tgattatctt ttttgttgct attttaacca
     9781 gccttgccac ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg cagtatcagg
     9841 ttgatggtca aacaaaaaca cgcaaagtcg tagatccaca ctcatttcgc attctgacta
     9901 acgaagcagg cgaacctgag tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc
     9961 cgggcctgat gaacttcccg tttgaaggat taacctcagg atcgaaatac gggacagccg
    10021 ttggcatcat catgtttatg ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag
    10081 gaaccattga taacggtatc ctggcgctta ttcgccatac ccgcgggaat gaaattctct
    10141 ttattcctgc gctgtttatt ctgttttcac ttggcggcgc ggtatttggt atgggagaag
    10201 aggccgtcgc ctttgccatt atcatcgcac cgctaatggt ccggctgggc tatgacagta
    10261 ttaccaccgt cctggtgacc tatattgcca cgcaaatcgg ttttgccagt tcgtggatga
    10321 acccgttttg tgtggtcgtt gctcagggga ttgccggcgt tccggtgctt tctggctccg
    10381 ggttgcgcat cgtggtgtgg gttatcgcca ctctgattgg cctgatcttt accatggtgt
    10441 acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct
    10501 ttcgtgaaaa gcaggctgat gttgaacaac gtccgtttac ctttggtgac tggctggtat
    10561 tgattgtcct gaccgccgta atggtctggg tgatttgggg cgtgatcgtt aatgcctggt
    10621 ttattccaga aattgccagc cagttcttca ccatgggtct ggtgattggc atcatcggcg
    10681 ttgttttccg ccttaacggc atgacggtta ataccatggc ttcatccttt accgaagggg
    10741 cgcgaatgat gatcgcccct gccctgctgg tgggtttcgc caaagggatt ttgctgctgg
    10801 tcggtaatgg tgaagcgggt gatgccagcg tgttaaatac catcctcaac agcattgcca
    10861 atgccattag cggtctggac aacgcggtcg cggcctggtt tatgttgctc ttccaggcgg
    10921 tatttaattt cttcgtgacg tccggttctg gtcaggcggc gttaaccatg ccgttactgg
    10981 caccgcttgg cgatctggtc ggtgttaacc gccaggttac cgtgctggca ttccagtttg
    11041 gtgatggctt cagtcacatc atttacccaa cctcagcttc gttaatggca actctcggtg
    11101 tatgcagggt ggacttccgt aactggctga aggtgggcgc gaccctactt ggactgctgt
    11161 ttattatgtc cagcgtcgta gtgatcggcg ctcagttgat gggctaccac taaaaatgtt
    11221 aagagccgca attgcggctc ttttttcatt ctgccgtttc agtctctacg gcttcatttt
    11281 tggcattgcg cttcatccac aacgccagcg ctttcagcga gtctggagtg aactcatcgc
    11341 agcgtgcggt gatttcttcc ggcgtcagcc agcaaacttc actgacttca tcttcctgta
    11401 gggcgaaggg accgtgagag acgcagctga acaacgcgcc ccagacacgg caatttttat
    11461 cttcgaaata gaactgcccg tgctcggcaa aggggacacc ggcaatgccc agctcttctt
    11521 ccgcttcgcg acgcgcggat tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg
    11581 tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt acgacgctgg accagaattt
    11641 tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg acgcagacac tgtgcccgca
    11701 tttgttcccg gctggcttgt gcaatgactt cgttctcttc attgacaata tccacccatt
    11761 cagtacttgc caaacgacgc tgttccatta tcaggaaacc ttctttttct ggcgctctta
    11821 cggcgcattt tgagttgtgg gtaaattacg gattaatcgc gacctgcgca atgatacttt
    11881 gatcattgag tgcgattaca ctaagtacgt cattatccag catgccataa ctcgccggat
    11941 taccgccttt cggaatactc accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt
    12001 ctgccactgg cagatgggta tgaccgtaca ccagcacatc gttctggttt aaagcaggta
    12061 gattttccgg gccaaaaaga tgaccatgcg tcaaaaacag acgttgtttt tccagtaata
    12121 cctgttgcca cggcgcggtt atcgggaaat gcagcagcat ttgatccact tcgctgtcgc
    12181 agttgccgcg cacagcgata accttatgtg ccacttcatt aagccgttca gcgactttgg
    12241 ctggcgcgta accctccggt aaagcattac gcgggccgtg attcaacacg tcgccaagga
    12301 tcaccagcca ctgggcaccg ctttgggcaa acaactccag aacacgttcc gtcgccggta
    12361 acgacccatg aatgtccgat gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac
    12421 gtaagctatg atactggatt ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca
    12481 ataaaacgct ggacagacgc tcgctgccac tggaatgtcg cataatccac caggcgttct
    12541 ggcacctcat cgccatgtag taccaggcgg ttaatcatta gcgccagatc agtatcagca
    12601 atgcaccatt caccaaataa attcggctga cccagtacta acaaatgttc tgccatcgca
    12661 aacagtttct ctgcactggc ttttccctcg gccgttagtg gcgctttttt cgcccccgca
    12721 aagacaacat ccgtcggacg ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc
    12781 tgaatctgtc gtgcacgcgc acgattttct aaatcaagcg gataaatacg ttcccaggtc
    12841 ggtggcgcaa atcgatcttc cagatactcc gcaatggcag aagattcact caactcaaaa
    12901 tcatcgattt gtaataacgg cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc
    12961 aaatgttcac cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc
    13021 aacgccaccc aggcggataa cacataaggg gagaaaaagt gggcatctga ccaaagcgtg
    13081 atagcgggtt tactcattat gtcctcggca ataaaggggg ctggctatca aactatagag
    13141 cctttttgtc actgtcacgt gacgaatact cacgtaattc gccgcgcttt ctatacttgt
    13201 ggtgttcaga tgattaaaca accggagctg caatgatcga tctctatttc gccccgacac
    13261 ccaatggtca caaaattacg ctgtttctcg aagaagcaga gcttgattat cgcttgatta
    13321 aggtagacct ggggaaaggc ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca
    13381 acaaaattcc ggcaattgtt gatcattctc ctgccgatgg cggcgaaccg ttaagcctct
    13441 ttgagtctgg tgccattttg ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg
    13501 aaacgcgtga gcgcgccgcc acattacagt ggttattctg gcaggtaggc ggactggggc
    13561 cgatgcttgg gcaaaatcat cattttaatc acgcagcccc ccaaaccatt ccttacgcta
    13621 ttgaacgtta tcaggttgaa actcagcgtc tttaccatgt actgaacaag cggctggaaa
    13681 actcgccctg gctgggaggc gagaactaca gcattgcgga tattgcctgc tggccgtggg
    13741 ttaatgcctg gactcgccag cgaattgacc tcgcaatgta tccggcagtc aagaactggc
    13801 atgagcggat ccgttcgcgc cctgccaccg ggcaggcact gctaaaagca caactcggtg
    13861 atgagcgttc ggatagttaa cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa
    13921 caacggagaa cctgcaatgg cacaacctgc cgctattatt cgtataaaga accttcgttt
    13981 acgtacgttt atcggaatta aggaagaaga aattaacaac cgtcaggata ttgttatcaa
    14041 tgtgacgatc cactaccccg ccgataaagc gcgcactagc gaagatatca acgatgcgct
    14101 gaattatcgc accgtaacga aaaacattat tcagcatgta gagaataacc gtttctcttt
    14161 gctggaaaaa ttaactcagg atgtgctcga tatcgcacgt gaacatcact gggtgacgta
    14221 tgctgaagtg gagatcgata aactgcacgc gctgcgctac gccgattcgg tatccatgac
    14281 cttaagctgg cagcgttaac cgccatatcg ggaggctgca tgaacatagt gatcaccgga
    14341 gggacgggat taattggtcg ccatttgatt ccgcgtttgc tggagctggg ccatcaaatc
    14401 acggtagtga cgcgtaaccc acagaaagcc agttccgttc tcggccctcg ggtgacgcta
    14461 tggcaagggc tagccgatca aagcaacctc aacggcgttg atgcggtaat caacctggcc
    14521 ggagaaccga ttgctgataa acgctggact cacgagcaaa aagagcgtct ctgccaaagc
    14581 cgctggaata tcacgcaaaa actggtcgat ttgattaatg ccagcgacac gccgccgtcg
    14641 gtactcattt ccggctcggc aacgggctat tatggcgact taggtgaagt ggtggttacc
    14701 gaagaggaac cgccgcataa cgaatttacc cataaactct gcgcccgctg ggaagaaatt
    14761 gcctgccggg cgcaaagtga caaaacgcga gtgtgcctgc tgcgtaccgg tgtagtgctg
    14821 gcaccggatg gcggtattct cggtaaaatg ctgccaccgt ttcgtcttgg cctgggcggg
    14881 ccgattggtt ccggtcggca gtatcttgcc tggattcata tcgatgatat ggtcaacggc
    14941 attctctggc tgctggataa cgagctgcgc gggccattta atatggtttc gccctacccg
    15001 gtacgcaatg aacaatttgc ccatgcgctc ggtcatgcgc tgcatcgccc ggccattttg
    15061 cgcgtccccg cgaccgccat tcgactgtta atgggcgaat cttcagtact ggtattaggt
    15121 ggacaacgcg cgctgcctaa acggctggaa gaagcgggtt ttgcgtttcg ctggtacgat
    15181 ttagaagagg cgctggcgga tgtcgttcgc tgatgtggtt tacagcaaac atccgccagt
    15241 taactcccgg tgttacagga ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg
    15301 gtcaccatca taactaactc tctgtctaaa cctctatcca gcatctcctg agcaatacgc
    15361 agggcttctt cgtgtttgcc ctgcattgcg ccttcttcac gtaatctgtc agcaatggtc
    15421 atcagtttct ccttttcttg tggtgcgcgt tccgctatct caccaataaa tgcacgaaaa
    15481 cgctgggcat cccctgtttg taatacgtaa ttaaacaggg cttttagctg tctgtcatta
    15541 gtgttccctg taactagcag cgaaacaatt tggtcgacta atcccaacag atcgcgctga
    15601 cgaatatgtt tctgaattaa ctccaacagc gccattttgc ggtgttgcat aatctcgtca
    15661 tccggcacca cggtaatatc caccaacgga aacgctgatg aatatatttt gtgggctata
    15721 gcaggctcgg caaattcatc aagccagcag agtgaataag gataaggact tctgcaacca
    15781 tgataaaaca gcatcgggat caccaatgga agctctttat agcccgcatc aagatggttt
    15841 tgcattgccg caatggaata acgcatcatg cgaaaagcca ttaattcttc cggcttactt
    15901 tggtgctcta tcactacata aatataaccc actccctcct gcgttttcac agaccacaag
    15961 aggtcggaat aatattgccg caggtcgtca tcaataaaac tgtttggttc cagtttaagc
    16021 gtcgttaaat cacacagttt gcgcagcggc gcgggaagat gaatatcaat aaaatcccgc
    16081 gcggtgtctg gatggcgtaa aaaagattta aataccgcat catgcggcgt ggaagttgtc
    16141 gatattgtca tggcgttccg tcacccctca aatttaagat gacgcgacaa taaccacgat
    16201 taacacccaa tgcagcaggc agtttttatc tttggacagc gaattcagaa gtatttttag
    16261 cggactgcaa cttttacatt tcagcggaag ctgactcgca aattccccat gaaaatactc
    16321 cggcagcgtt agccaccgga gaggaaatta tttcagcgat cccttaagga actgttgcag
    16381 acgagggctt tgcgggttgc caaataactg ctccggcgcg ccctcttctt ctattttccc
    16441 ctgatggagg aaaatgacat gagtagaaac atggcgagca aagcccattt cgtgagtcac
    16501 taccaccatg gttttcccct cttctgccag ttgctgcata atacgcaaca cttcgcctac
    16561 cagttcagga tcgagcgccg aggtaggttc atcaaacagt aaaacttccg gttccatcgc
    16621 cagcgcccgc gcgatagaaa cacgctgttg ctgaccgccg gaaagatgca ccggatattt
    16681 cccctgcgca cgttcgtcta tcccgacttt tgccagatac ttgaccgccc gctcgcgcgc
    16741 ttcctgcttg ctcaggccca acacctgaat cggcgcttcc atgacgtttt ccagcaccgt
    16801 catatggctc cagagattga agtgctggaa taccatcgtc aggcgtgtgc gcagtaagcg
    16861 cagttgattt ttatcggcga ctttgagttg accgtctttg tcgcgcacca gattgatcgt
    16921 ctggccattg accacgatcg acccttcact cggtttttcg aggaagttaa tgcagcgcag
    16981 aaaggtactt ttccccgatc ccgacgatcc gatgatgctt attacatctc cggcattcgc
    17041 ttgcagtgag acccctttca gcacttcatg ttcgccgtag cgtttgtgca aatcgataac
    17101 gtttaattta ttctcggaca tcgtattctc agtgcgttga agaaggtttc acatgctgca
    17161 accagcgttt ttccgctctg cgaaagaggc tgatcaggac ataagagatg attaaataga
    17221 gcaccgcggc aatgccgaag gcggtaaaag gttgatacgt ggcggcgtta atatcgcggg
    17281 ctattttcag cagatccggc accgtggccg taaacgccag cgcagtagag tgcagcatca
    17341 ggatcacttc gttgctgtat gccggtaacg caatacgcag cgcagaaggc aaaataatgc
    17401 aacgatacat tttaaaagtc gagaagccat aggctctggc ggcttcaatt tccccatgcg
    17461 gaaccgaacg gattgcccca gcaaaaatct cggtagtgta agcgcaggtg ttaagcgtca
    17521 gcgccagcac ggtacagttc aggccactgc ggaagaaagc gttaaggaat tcggttccct
    17581 taacaatctc aagcgtgtac atgccggaat agaacaccag caactgaaca tacagcggcg
    17641 taccgcgaaa aatataggta aataaccaga ttggaaactg gatgtattta ttactggaga
    17701 cacgaccaat cgccagaaac agcgccagga ctccgcctat cactaccgac aaaataagca
    17761 gccacagagt gatcgccaca ccagtaaagc gataaccgtc ggtccacagc agcggtttcc
    17821 agtattcatg taagatttcg atcacaggtc agccctcttc acacccacgg agtagcggcg
    17881 ctcaaggaac agcagcacac cattggaaac agtggtgaaa accaggtaaa tcacgccaca
    17941 gacgatggcg aaatagaacg gttcccaggt acttttgcct gccagttggg tggctttgac
    18001 cacatcttcc aggccgagta acgaaaccag tgcggtagat ttgaggatca cctgccagtt
    18061 gttgccaatg cctggcaggg cgtaacgcat catcgccgga aacatgatcc gccgaaacac
    18121 ttgcccacga gtaaaaccga acgccgtcgc cgcctctata tgccctttcg gcactgccat
    18181 aaacgcgcca cggaacgttt cggtaaaata agcaccgtag ataaaaccga gagtaataat
    18241 accagcgacc atcggatcga tatcaatctg cccgacgccc atcgcctccg tcaccgtgtt
    18301 tagcgcaatc tgcaaaccgt agaaaatcag cagcatcaac accaagtccg gcacgccacg
    18361 aatcagcgtg gtgtaccctt cgaaaatcag ccccgaaagc cgattttgcg agagcttacc
    18421 gccagcgcca attaaaccga tgattacagc gagcactaca gaactgatag ccagctccag
    18481 cgtgacgagc gcaccctgta aaataacacc tgaaaaccca tacaacatgc tgcctgtcct
    18541 gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt tacccgaagg gagcgggctt
    18601 tctttcaccg atggcgatta accgccataa acatcaaaat cgaagtactt tttcgctaat
    18661 ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt tgttcagtgc ctcgcgcagt
    18721 tcgttatctt ctttacgcag gcccatgccg gtccctacgc caaacagttt ttcatcttta
    18781 acagacgggc caccgaattt gtaatcttta ccgacgggtt gtttgaggaa accttcactg
    18841 gcagcgacct catcctggaa cgcggcatca atacgtccgg cagtcaggtc agaataaatg
    18901 ttgtcctgcc cctgatacga gacgatttca atgccttttg gtgcccaatg ttcattaccg
    18961 aacgtctcct gggtggtgcc ctgcaatacg cctacccgtt tgcctttcag cgactcgact
    19021 gtcggctgaa tgtcagaatt tttcgccacc accaaacgag aatcggcagc gtacagtttg
    19081 tcggtgaagg ctatttcttg ctgacgtttt tccgtaatgg aaagcgatga catgatggcg
    19141 tcaatcttct tcgcttttaa ggacgggatt aacgcatcca gcggattttc gacaaacgta
    19201 cattgcgtat tgatgcgttt gcataattcc tttgccagat cgatatcgaa gccaaccagt
    19261 tcgccttgtg aattttttga ttcaaatggc gcataggtcg ggtcggtacc gatgcggatg
    19321 ttttgcggaa tcgcagcaaa cgccgcagtt gcgctggaga aggccagaac cagagagagc
    19381 gatagcacca gttttttcat aatgtcctca actgacagtc tttttatagg gaatatttac
    19441 aggtttgtta cacttatcgt gccataaaat tgaccatcaa ggcaaaatat cccgccccgt
    19501 tagacgtatt atttttcatt aaaaatgctt aagtatgcat ttcaatgcac cacgatagtg
    19561 caacaaacct ggcgtgcacc atgatgatca ttccatcagg tacagcttcc cagcgacgta
    19621 tcagtcaccg tagacattaa agtcgaaata ctttttcgcc atcttgtcgt aggtgccgtc
    19681 ctgacgcagc tcgccaagcg ccttattgaa ggcagccgtc agttcagcat catctttacg
    19741 tagccctaca ccggtgccat caccgaagta ttttttgtct tttactgatg agccagcaaa
    19801 ggcgaaatct ttaccagcag gttgcttgag gaatccttcg ctggcagcaa cttcatcttg
    19861 taacgcagca tccagacgtc ctgcagccag atcggaatag accaaatcct ggttggcata
    19921 ggccaccaca tccacgcctt tgctacgcca ggtctcgtta gcgtaagctt cctgggttga
    19981 tccctgcagc acaccaacat gtttaccttt cagtgaatcc agcgttggct gaatcggtga
    20041 acctttggca gcaatcaaac gagaatctgc ggcgtacagc ttgtcggaga aggcaatctc
    20101 ctgctgacgt ttatcggtaa tggaaagcga cgaaataatg gcgtcgattt ttttcgcttt
    20161 cagtgagggg atcagcgcgt caaagtcact ggcaacccag gtacatttca cctgcatccg
    20221 tttgcacatc tcgttaccga gatcgatatc aaagccaaca aaatcacctt tagcatcttt
    20281 cgacgagaac ggcgcatagg tggtatcggt tccgatacgt accgtctccg gtagcgccgc
    20341 atagctggaa gccgctgcgg agagaccgac taacaaagac agagcgagaa tcgacttctt
    20401 catacataac cctcaagtga aaatggcttt tttatgttgt gtattgtgtt gtgtgtttgc
    20461 aggctctttc atgcaggtct tatgccatct tgacgacaac agtaacattc aacgttaaat
    20521 atgttaataa gacgttgcat tattgtcctg aagttgaaga tagcaggtat agcggttgaa
    20581 tcgcagcgtt tcgattgaat ggcagaaaca aaatgtcgag gatttgatcg cggttgcaaa
    20641 attgccctga aacagggcaa cagcggagtt atgcgccctg ccagcgggca aagagatctt
    20701 caggaagggt tatcgcaaac tggtcaagaa cacgattaac cgtctgattt atcacatcat
    20761 caagggattg cgggcgatga taaaacgccg gaacgggagg cataatcacc gcaccgattt
    20821 ctgccgcctg agtcattaaa cgcagatggc ctaagtgcaa tggtgtttca cgcacgcaga
    20881 gcaccaacgg gcgacgctct ttcagcacca catctgccgc acgggtcagt aagccatcag
    20941 tatagctatg gacaatgccg gaaagggttt tgattgaaca gggtaaaatc accatcccta
    21001 gcgtctggaa agaaccggaa gagatgctgg cggcaatatc gcgcgcatcg tgcgtgacat
    21061 cggctaatgc ctgcacttcg cgcagagaaa aatccgtttc gagggataag gtctggcgcg
    21121 ctgcctggct catcaccaga tgcgtttcga tatctgtgac atcgcgcaga acctgtaata
    21181 agcgcacgcc ataaatcgcg ccgctggcgc cgctgatgcc tacaatgagt cgtttcataa
    21241 atagttgccc tgtcagactt gcaggcagac tttgcaggat ttcggcggga gttgcaagtc
    21301 agggtgccag accggcaccc tcagcgaagg catcatcctt cgttatgcat ttcgagattt
    21361 tccacttcgt tctgacgttg cactgctttg gcgtcatcat tacgtaacgt atcgaggaaa
    21421 tcgaggtagc cctgatcaac atctttggtg acgtagacgc cgttgaacac cgagcattca
    21481 aactgctgga tatccggatt ttcagcgcga acggcgtcga tcagatcgtt cagatcctgg
    21541 aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat caacttcgcg accgtgagcg
    21601 atcagttccg tggcgctcgg catatcaata ccataaacgt tcgggaagcg aatttccggt
    21661 gccgcagaag cgaggtacac tttcttcgct ccggcttcgc gtgccatctc gataatctgc
    21721 tcagaagtgg tgccacggac gatggagtcg tcgaccagca ggacgttttt atcgcggaac
    21781 tcggcgcggt tggcattcag tttacggcgc acggacttac gacgcagctg ctggcccggc
    21841 atgataaagg tgcggccaac atagcggttt ttaacgaagc cctggcggta cggtttgccc
    21901 agaatacgag caatttccag cgcgatatca cacgaggttt ctgggatcgg gatcaccacg
    21961 tcgatatcca gatcttccca ttcgcgggca attttctcgc ccagtttcgt gcccatattc
    22021 acacgcgcgc tgtaaacgga aattttgtcg atgaacgagt ccgggcgagc aaagtataca
    22081 tactcaaaca ggcacggatt gctgaccgga ttgtcagcac attgacgggt aaacaactgc
    22141 ccttcttcag tgatgtaaat cgcttcgccc ggcgcgacgt cacgcaggaa atcaaagccc
    22201 agcgtatcga gcgctacgct ttcggaagcg accatatatt ctgtacggtt ctcgtcaata
    22261 tcacgttttc ccagtaccag cggacgaatc ccgtttggat cgcggaaagc aaccataccg
    22321 tggccgataa tcatcgccac acaggcatat gcgccgcgga ttaagcggtt tgtggcagca
    22381 atggcagcga aaatattgtc ggcttccagc gggtagtggc ggaagttgtc cagctcgcta
    22441 gcgaagatat taagcagaat ttccgagtcg gaagtggtgt tgatgtggcg gcgtttttct
    22501 tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat tgccgttgtg ggcaagcgta
    22561 atgccatacg gagagttaac gtaaaacggc tgcgcttcag aggcgctgga gctgccagcc
    22621 gtagggtaac gcacatgacc aatgcccata ttgccctgca aacgctgcat atggcgagct
    22681 tcaaatacat cgctcaccag cccgttcgct ttacgcaaac ggaagcagtt attggcatct
    22741 atggtgatga tgccggcggc atcctgaccg cgatgctgaa gcaccgttaa ggcatcataa
    22801 atcgactggt taaccggcat aacaccggcg ataccgacaa taccgcacat acgtcttttt
    22861 cctcgttaag ccacatctca gagcacttac gctctgggca agaaacttga cgagctttgc
    22921 agataatcaa aaaaccatct gatgataaaa ctgaactgcg ggatcagctg tgatttgctc
    22981 cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa agaagagaat ggcggcaaca
    23041 atcaacacac cgcgcaacgc accgaaacag acgcccagca cccgatcggt gcctgacaac
    23101 cccgttttct ccaccaactg gcctatcacg aagttcacga tagcaccaac gatcagggta
    23161 gcgataaaca gtaccgcgat ggcaattcca tttcgaacca gttcgtcttc aaagcccgta
    23221 aaccagactg acaggtaagt gtagtaatga ctggcaacaa agaaagcaca accccatgtc
    23281 accagcgata acgcttcacg aacaaagccg cggatcaggc taaccagaga ggaaaaagca
    23341 atcaccgcga ttatggcgta atcaatccag accatatgtg tcccacgatt ttacgccctg
    23401 tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg tttgcgtagg gatttccttc
    23461 ccgcgcatca ataaaaatgg cgctgaaaaa atattcaacg ccatcgactt tttatgcctt
    23521 tgcggcatcg tgcaatgcgt gtcggatgcg gcgtaaacgc cttatccgac ctacggttct
    23581 aacactgccc aggcctgata agacgcgcca gcgtcgcatc aggcaagact gtattaattc
    23641 ggcgtatagc ccattaccac gccacttaag ccagaaagtt gcttcaactc acccagcgaa
    23701 cctttcagct tatctttcga ggcatccggc ccaaccagaa tacgggtaat tttaccctgc
    23761 actggcgtgg atggcgacgt ataaacccga taaccggcac cgcgcagctt accgacaatc
    23821 tcattcactt tatcggcatt tttcagcgca cccagttgca caacataggc tttacccgtt
    23881 ggcgctgctt tttcttccac gaccggcttc ggttctggct tcggcgcagg tggtgcttca
    23941 accttcggtt taggcggctc caccggtttc ggcttcggtg gggcgaccgg tgcaggttcc
    24001 ggttcaaact cggtgttatt ggctgcaata gtggtcggat cgagcgacgg tgctgcggca
    24061 tcacctgccc gcacctcttc cgctgcgcct tccggcggct gcgtcggcaa cgcctgggtg
    24121 gcggctggca tcatatcagg ctcatcgcga tcgcccgctt tcggcaccag cgggatagcc
    24181 gcgaactcat cctgataatg ttttttctgc ccgtccagca gccctggaag tacaatcacc
    24241 cccagcgcca ccagcacgat cgtgcccact aaccgattct gaaacttact tgccaccgct
    24301 tctcctcgcg tcaatcactt ccatgacatg tgcgacagtg tggaaagaac cacacaccag
    24361 cacggtgtct tccgctttag cgtccgccat tgcggcatcc catgcctgcg caacgctatc
    24421 aaatgatttg ccgttaccca aatgctcaag cagttgttct gccgtggcac cgcgcggccc
    24481 ttccagtggc gcacaatacc agtcatcaac cacgcttttc aaccaggcca gagttccggc
    24541 aatatcttta tcatgtagca taccgataac cgccagcacg cgcccgtttt tcggtagcgc
    24601 tttcatacgc ccggtgagat attccgccgc atgtggatta tgcgcgacat caaaaataac
    24661 gcgtggcgac tcgctcacaa tctggaaacg tcccggcaaa attgcgctgg caatcccgtc
    24721 gcgaatggca ttttcactga cttccagccc gctggcacgc agtgccgcca gcgccgttgc
    24781 ggcattcggt tgcgggacaa gcggcaacgg cagattttcc agcgtgccgt gagcatcgct
    24841 aaacgcccag tcatgatcgg tgacggaata gttccactca acgccccgac gttgtaacag
    24901 tgcacctttt tcctgtgcca catcagcaat ggtagaaggc atttccggct caccgacaat
    24961 tgccggtttt tcgctgcgga agatgcctgc tttctcgcga ccaatacttt cgcgatctgg
    25021 acccagccag tcggtatgat ccagcgcaat actggttact accgcgacat cggcatcgac
    25081 aatattggtt gcgtccagac gaccgcccag ccctacttcc agaatcacca cgtcaagttg
    25141 tgcctgcttg aacagccaca acgccgacag cgtaccgtac tcgaaatagg tcagggaaat
    25201 atcaccgcgt gccgattcaa tctccgcaaa agaggcggtg tgggccgatt ccggcaattc
    25261 ctggccctgc acacgtacgc gctcggtata acgcaccaga tgcggtgaac tgtagacgcc
    25321 cactttgtac cctgccgcca tcagaatcga ctccagcgta cggcaggtgg tgcctttgcc
    25381 attcgtaccc gcaacggtaa acacaaatgg cgctggtttc agaacgccaa gacgcgccgc
    25441 gacctggctc acgcgctcaa ggccgagatc gatagtttta ctgtgcaggt tttccagata
    25501 agaaagccac gaagccagag gcgacgcggc ttgaggagtg cgtttgataa tcatggtatc
    25561 cgctgattcg ttacggtgag aatagcaaaa gggcagagcc agtggccctg cccttatcag
    25621 ttatcaggcc tcaggttcct gatccggtac cgggggtacc actacgcctt cacgcggcgc
    25681 ttcaggattc ggcgctggca gattcatcaa cttcgccaga atgctcgcca gtttcaggcg
    25741 catttccgga cgacggacga tcatgtcgat ggcgcctttc tcgatcagga attcactgcg
    25801 ctggaatcct ggcggcagtt tttcgcgaac ggtctgttcg ataacacgcg gaccggcaaa
    25861 gccaattaac gctttcggtt cagcgatgtt gagatcgccc agcatggcga aacttgcaga
    25921 aacaccgccc atcgtcgggt cggtcagcac ggagatgtac ggcaagccgc gctcctgcat
    25981 ttttgccagt gccgcagagg ttttcgccat ctgcatcagc gacatcagtg cttcctgcat
    26041 acgtgcgcca ccagaggcgg agaagcagat cagcgggcag ttatcttcca gcgcctgctc
    26101 aacggcacgc acgaaacgtg cacccacaac agaccccatt gaaccgccca taaaggcgaa
    26161 ctcgaatgcc gcagcgacaa ccggcattcc atacagagtg cctttcatca ccaccagcgc
    26221 atctttttcg ccagtttctt tctgcgcaga tgccagacgg tctttatact tcttggagtc
    26281 acgaaacttc agcacatctt tcggctcaag ctcgctaccc agctccacaa ggcttccttc
    26341 atctaacagg ctatgcaggc gattacgcgc tgtcatacgc atgtgatggt cacacttcgg
    26401 acagacctca agattacgtt ccagctcagc gcggtataaa acctgaccgc agctatcaca
    26461 cttagtccac accccttcag gaatgctcgc cttgcgggtg ggagtaatgt tgcttttaat
    26521 tcgttcaatc cagctcatta gggacctttc tgtctgaacc tggttcgatg ccagttttat
    26581 ctttggggac gcataatgcc atttttgccc ccaacagacc atgaatgttg cacattaaaa
    26641 cataacagcc cgaaactttg gataaaaaag tggtcgaacc gcggagttac tttttatttt
    26701 gcggcgcgtg ccgcagcgcg tttgtgacga ataaattcga aaaccccagg caatattgaa
    26761 acaaaaataa tgccaacaat catcagctta aggttgctct gaatgaaggg gagcgtgccg
    26821 aagaaataac ccgcgtaggt aaaaatcagt acccacaaca gtgcgccgat cacgttataa
    26881 gcggcgaaat gacggtacga catgtggccc attcccgcaa caaacggcgc gaacgttctg
    26941 acgatcggta caaaacgggc gagaataatc gttttgccgc catgtctctc gtaaaactga
    27001 tgagtcttat caagataact gcgacgaaag attttcgaat ccgggttact gaacagtttg
    27061 tcaccaaata accgtccaat ggtatagttc actgcatcac cgacaatagc ggcaatcaac
    27121 ataagcacca ccatcatatg gacattgaga tcgttagttt ccagcgacgc caatgcacca
    27181 gcaacaaaca gcagcgaatc accaggcagg aatggcgtta ccaccagccc cgtttcgcag
    27241 aacagaatca aaaacagaat ggcataaacc cagacgccgt actccgcgac cagctctgcc
    27301 agatgcacgt caatgtgcag gataaaatcg atgaggaaat aaatcaggtc catatttgcc
    27361 tatgccttgt actcgtcatc tttcaggctg taactacgtt ggctacagcc tgaaatgttc
    27421 cgagcattat tctcgttagt ccgccagaaa tagcgggccc attggcggtt ttggaagatc
    27481 ataccggtca gggtaatcca ccgcgaccag atacagccct tccgcttttg ccgttgctgc
    27541 cgccagcgtt ctgtcctttg ccgccagcaa ctctgctatc cagctctccg gctggttgtg
    27601 ggcaccgact tccatcaggc tgccgacaat attcctgacc atatgatgta caaaggcatt
    27661 cgctttgata tctaccacca cataaggacc gtgacgcgtg acattaatat gcataacgtt
    27721 acgccacggg gttcgggact ggcactgcac cgcacggaac gaggtgaaat cattctcgcc
    27781 cagcaagcat tgcgcagccc gatgcatccg ttcagcgtcc agcggttcgt aaaaatgggt
    27841 taccccttta ctcagtaccg ccgggcgcag ccgatgattg tagatgatgt agcgataacg
    27901 gcgagccgtg gcgctaaatc gggcatgaaa atcatcaggt acagctttaa cccaacgcac
    27961 ggcgatgtca ccaggtaaat tcgcatttac gcccaaggtc cacgccgcgt ctttgcgctg
    28021 cgcggttgtt tcgaaatgca ccacttgccc ggtaccgtgt acccctgcgt cagtacgccc
    28081 ggcgcagaag acggtgatgg gttcgttcgc cacctgggag agcgcctttt ccagcttctc
    28141 ctgcacactg cggacttcat tctgccgttg ccagccgtaa tacttactgc cgtcgtactc
    28201 aatgcccagc gcaattttat aaactggcag ttgttgctgg tcggacatta gtacagatac
    28261 tcctgcacca gtttctcggc gatttttact gccatcagcg cgccgccaaa gcgaacgtta
    28321 tcggccaccg accagaactg gacttgctcc ggcataccgt agtcattacg cacgcagcca
    28381 acagaaagat gcggcgtacc cgaagcatcc cctacctgag tcgggaattc gttctcttca
    28441 gagagcacaa tatcttcgcc ctgagcaaac gcatcacgcg cttcttctgc cgccagcgga
    28501 cgcagtgctt caaagttgac catctgggca tgaccgtaga ataccggtgc ctggacgacg
    28561 cttgccgaaa tcatcagccc ttcgtcctgc aggattttgc gtacttcgtc aacgatacga
    28621 cgttcttcac gcacgctacc ttcgctatcc ggcaacaacg gcagcatatt gaacgccagt
    28681 tgacgaccga agaaatcttc ttcgtcaatc ggaatgccgt tgagcaattt cgcactctgc
    28741 cccgctaacg catcgaccgc ttttttgccc tgggcggagg ctgaaatcag gctggtaaca
    28801 ctgatacgcg acaaaccgcc ctgatcgatt aacggtttca gtgccgccag cagctggctg
    28861 gtcagactgt ctggtacggc gatgacattc cggttgcggt aatcggtcag tacaaacggg
    28921 tttacttccg gcaccaccag cggtacgtcg ggttcgagag caaacagtcc gctgctgtcg
    28981 atcaccaggc aacctgagtt ggtcgcttct tcaacccagg cagcggtagc ttctttgcct
    29041 gcgacaaaaa atgccagctg cgcctgcgtc cagtcgaatt cagcggcatc ctgcacggtg
    29101 attgtcttac caccaaagcg cagttgttcg cctgcgcttt cgttacgtgc cagtgcataa
    29161 atttccccaa ccgggaactg acgttcagcc agcgtttcaa gcagggcttc gcccacagcg
    29221 ccagttgcgc ccaggacggc aatgttccag ccttcagaca tggtggttta ctccagaaat
    29281 agcaaagctc cctgccaatg ttacagcaga gagcatgaag aagagattaa cgtgccggat
    29341 gatgaacggc gttaaaaccc agtttacaca gcaatgatgc cgcactggcg tcatcacaaa
    29401 ttacatacag agatgaccat tcacggcgct caagatagtt tttgcgcagt ttatcgaact
    29461 cacccggtat cccggcgact ttacgcagcg gtgcgtcatc gcggcgcaca tcatacacca
    29521 aatgcaccag ccttttcagc gtcggttgat cgagcgggcc atgcagcgta atgcgaccaa
    29581 actctggcgc gggcagtaat gtatccagcg caacgtgctg ttcatgccca ataaacttgc
    29641 tataggcttc aaacacttgc gtagtaccgc gtgctttacc ttccagggta taacctgcga
    29701 tatgcggcgt gccgatatcc acttttgtca gcaactccac gttaagttcc ggttcgcctt
    29761 cccagacatc cagcactacg cttaacttct ggccttcatt caggcaggtc agcaacgcag
    29821 tattatcgac gactgcgcca cggcaggcgt taatcagaat cgctcccggc ttcaggctac
    29881 ggatcagttt ttcatccgcc agatgcagcg ttttgtacgg cccatctttg aagagtggcg
    29941 tatggaaagt cagaatatcc gcatgctgaa ctaattcatc cagcgagcgg aaatcaccct
    30001 catccccacg gtcagcgcga ggcggatcgc aaagcaatgt tttaatccct aacgcttcca
    30061 gtcgcgcctg caatcgacgt ccaacgttac caacgcccac gatccccacc gtacggtcgt
    30121 gcagtgaaaa tccatcgcgt tcggcaagca tcagtagtga ggagaaaaca tattccacca
    30181 ccgcaatcgc attacagcca ggtgcagcgg aaaaaccaat tcccgcctgc ttcaaccatg
    30241 cttcatcgac atggtcggtc cccgcagtgg cagtgccaac aaatttaatg ggttttcctg
    30301 ccagcaaaga ttcattcact ttcgtgaccg aacgcaccat cagcgcatcc gcgtctgcca
    30361 gttgagcaac ggggattgga cgcccgggaa ccgcggtcac ctcacccaaa cggctaaata
    30421 agtcgcgggc ataaggcata ttttcatcaa caaggatttt cacgtttgtg ttacctgtat
    30481 gagacgagag ttaaccggac aagtgtgcca taatctcgcg gccaggcata cttgcgaaga
    30541 tttcaggtat aaggatacgt aatgatacaa cctatttccg gccctcctcc tgggcaacca
    30601 ccaggtcagg gagataacct gccgtctggc gcgggcaatc agcctttatc cagtcagcaa
    30661 cgtacttcgc tggaaagctt aatgacgaaa gtgacctcac tgacgcaaca acaaagagca
    30721 gaactgtggg cgggtatcag gcacgatatt ggtctgtcgg gagattcacc gctgctttcg
    30781 cgtcacttcc ctgccgctga gcataatctg gcgcaacgtc tgctggccgc gcaaaaaagc
    30841 cattctgccc gccagctttt agcgcaatta ggggagtatt tacgtctggg gaataatcgt
    30901 caggcggtca cggattatat ccgtcataac tttggtcaga cgccgctgaa tcagctctca
    30961 ccggagcaat taaaaaccat tctcaccctg ttgcaggaag ggaagatggt tattccgcaa
    31021 ccacagcagc gcgaggcgac cgaccgtcct ttattaccgg cggagcacaa tgcgctaaaa
    31081 cagctggtga ccaaacttgc ggcggcaacg ggggaaccca gcaaacagat ctggcaatcg
    31141 atgctggaac tttccggggt gaaagatggc gagttaattc cagcgaaact gtttaaccat
    31201 ctggtgacct ggttgcaggc gcgccagacg ttaagccagc aaaatacgcc gacgctggaa
    31261 tcactacaga tggcgctaaa acaaccttta gatgccagtg aactggcggc gttatcggca
    31321 tatatccagc aaaaatatgg cctttctgcg caatcatcgc tttcttctgc ccaggccgag
    31381 gatattctta atcagcttta tcaacggcgg gttaaaggga ttgatccgcg tgatatgcaa
    31441 ccgctgctta atccttttcc accgatgatg gacacgttgc aaaatatggc aacgcgtccc
    31501 gcgctgtgga tactgttagt cgcgattatc ctgatgctgg tctggctggt tcgttagccc
    31561 cgacgaaatg acagtatcgt gacaataatt cccagcaccg cagagatcgc cccggcaaga
    31621 aataccgaag agtaaccaaa cgtggtcgcc agcattcccg ccagcggccc ggagacgccg
    31681 agggcgatat cctgaaacgc ggcgtaaccg cccagtgcgg tgccgcgaac ttgtgagggg
    31741 acgcgtttaa ccacctccac gcccagcgca ggaaagataa gcgaacatcc ggctccggtt
    31801 aacgccgcgc ccgctaatgc gacccacgca cctggggctt gccagagcag caacaagccc
    31861 accgtttcta caagcagaga gacaatcgcc actttcacgc cgccaaaacg gtccggcatc
    31921 cagccaaaca tgacgcgcat cacgacaaat gcgccgccaa acgcggtaag agtaaagccc
    31981 gccatcgccc atcctttgct ggcaaagtag agcgaaacga aagtcccgat aaccgcaaaa
    32041 ccaacgcctt gtagtgccag ccctaaccct ggtttccaga taaacccgac aacgctccac
    32101 agcgatggac gttctcccgc cagggccggt actttgcgca ctgtgccgtt acaggcccac
    32161 gccagtaagg gtaataccat tgtggtgatc gccagtgcgg caaaaccgta atggctatga
    32221 atcaacaggc caagcggagc accaacagcg agggcaccgt aaatcgccat tccattccat
    32281 gacatcactt tgccagagtg ttttggccct acgatgccta acccccaggt cagagcgcct
    32341 gtcagtaact ggctttcacc aaacccaaga atcaaacgcc cgatgaccaa cagggcaaat
    32401 ttgaacggtg cggagacagg caaaatcgcc gccagcagca acgcgccgcc agccagacca
    32461 caagctaaca tcccctgaag cgccgaacgt tttgcaccat attgatcggc cagtcgcccg
    32521 gcatagccac gcgtcagcac cgtagccaga aactgaatcc cgacggcaat cccgaccatg
    32581 gtattgccat agcccagatc atgatgaaca aacagcggga taaccggcaa cggcagccct
    32641 acggtcatgt aggtgagaaa aaccgcaaaa gcgatgcgga agagcgaaaa attggcagaa
    32701 gatcgtgttt cggtttggct tacagcagtc atgcattact ccagaatgca gcgcaaggcg
    32761 aggagtatcc ccgtctcatc tctctggttt cagggttaca gtgcgttggc aggatttaac
    32821 gcgtacgtct tttcagaagg aaatcgacaa agcgggaagt ttgcctggaa ctggcggcga
    32881 ttgtcaatga tgtgaaaaag ggaaccatca ggttcccttt tgcgttagtg ccggaggccg
    32941 cattggcgcg taacgtcgga tgcgacgctg gcgcgtctta tccgacctac tccgactact
    33001 ccgacctact gcgaattaat ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc
    33061 gaagccgaag ctgttagaca taacggtagt cagttcgcga tcggtcgttt cggtcacgat
    33121 gttcagaccc gcagcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaagcc
    33181 gtgttccagc atcagcagag agtagatagc ttcctgtacg ccagcagcgc ccagagagtg
    33241 accggtcatg gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat
    33301 agctgccagt tctttcacgt cgccaaccgg agtcgaagta ccatgagagt tcaggtaatc
    33361 gattggggta tcaacgccgt gcatcgccat cttcatgcag cgtactgcgc cttcgccaga
    33421 cggagcaacc atgtctgcac catcagaggt tgcgccgtag ccaacgattt cagcatagat
    33481 gtgagcacca cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccgccgcc
    33541 agcgataacg aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc
    33601 gttgtattta gtagacagcg cacccatcgc gtcgaattcg caggccattt cccagcacag
    33661 ctcttcgccg ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc
    33721 gttaccgata cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccatg
    33781 aattttaaac ggcgtggcga ggcaggcaga aacgccggat gccatcgctt tggtgaccac
    33841 atacgggcca accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc tgaacacctg
    33901 gaaacgcggg gaaccgccgc cagaacctgc aatcaggcca acgcgcgggt tattctggta
    33961 agcttccgga gaaaggcccg catctgcgat tgcctgctcc atagaaagga atgcataaat
    34021 ggatgcgtcg ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt
    34081 tacgttgccc cagacgtggc tacgcatgcc ggaatccttc agctcctgag agaaagtgat
    34141 ccctgaacgt ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga
    34201 aacaatgccc aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat
    34261 agagtaagtt tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca
    34321 tttaatagag aaatttgcgc agccttacac acatcgctaa gatcgagcca ccgcctgtaa
    34381 gacgagtaac ttacgtgaaa cactactcca tacaacctgc caacctcgaa tttaatgctg
    34441 agggtacacc tgtttcccga gattttgacg atgtctattt ttccaacgat aacgggctgg
    34501 aagagacgcg ttatgttttt ctgggaggca accaattaga ggtacgcttt cctgagcatc
    34561 cacatcctct gtttgtggta gcagagagcg gcttcggcac cggattaaac ttcctgacgc
    34621 tatggcaggc atttgatcag tttcgcgaag cgcatccgca agcgcaatta caacgcttac
    34681 atttcattag ttttgagaaa tttcccctca cccgtgcgga tttagcctta gcgcatcaac
    34741 actggccgga actggctccg tgggcagaac aacttcaggc gcagtggcca atgcccttgc
    34801 ccggttgcca tcgtttattg ctcgatgaag gccgcgtgac gctggattta tggtttggcg
    34861 atattaacga actgaccagc caactggacg attcgctaaa tcaaaaagta gatgcctggt
    34921 ttctggacgg ctttgcgcca gcgaaaaacc cggatatgtg gacgcaaaat ctgtttaacg
    34981 ccatggcaag gttggcgcgt ccgggcggca cgctggcgac atttacgtct gccggttttg
    35041 tccgccgcgg tttgcaggac gccggattca cgatgcaaaa acgtaagggc tttgggcgca
    35101 aacgggaaat gctttgcggg gtgatggaac agacattacc gctcccctgc tccgcgccgt
    35161 ggtttaaccg cacgggcagc agcaaacggg aagcggcgat tatcggcggt ggtattgcca
    35221 gcgcgttgtt gtcgctggcg ctattacggc gcggctggca ggtaacgctt tattgcgcgg
    35281 atgaggcccc cgcactgggt gcttccggca atcgccaggg ggcgctgtat ccgttattaa
    35341 gcaaacacga tgaggcgcta aaccgctttt tctctaatgc gtttactttt gctcgtcggt
    35401 tttacgacca attacccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct
    35461 gggatgagaa aagccagcat aaaatcgcac agatgttgtc aatggattta cccgcagaac
    35521 tggctgtagc cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca
    35581 gcggcattac ttatccgcaa ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc
    35641 tggaactggc gcaacagcag ggtttgcaga tttattatca atatcagtta cagaatttat
    35701 cccgtaagga tgactgttgg ttgttgaatt ttgcaggaga tcagcaagca acacacagcg
    35761 tagtggtact ggcgaacggg catcaaatca gccgattcag ccaaacgtcg actctcccgg
    35821 tgtattcggt tgccgggcag gtcagccata ttccgacaac gccggaactg gcagagctga
    35881 agcaggtgct gtgctatgac ggttatctca cgccacaaaa tccggcgaat caacatcatt
    35941 gtattggtgc cagttatcat cgcggcagcg aagatacggc gtacagtgag gacgatcagc
    36001 agcagaatcg ccagcggttg attgattgtt tcccgcaggc acagtgggca aaagaggttg
    36061 atgtcagtga taaagaggcg cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa
    36121 tggtaggcaa tgttcccgat tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc
    36181 agaaagatga ggcggtaagc gcgccggttt ttgacgatct ctttatgttt gcggctttag
    36241 gttctcgcgg tttgtgttct gccccgctgt gtgccgagat tctggcggcg cagatgagcg
    36301 acgaaccgat tccgatggat gccagtacgc tggcggcgtt aaacccgaat cggttatggg
    36361 tgcggaaatt gttgaagggt aaagcggtta aggcggggta atctgctctc gcagtgtttg
    36421 ccggatgcgg cgtgaacgcc ttatccgacc tacgtgtgac
//
LOCUS       DAZFWP020000033        35874 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFWP020000033 DAZFWP020000000
VERSION     DAZFWP020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.033"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..388
                     /locus_tag="ACHK0Z_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             <1..388
                     /locus_tag="ACHK0Z_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501530.1"
                     /translation="KRYIKWFAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKNA
                     ILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDGK
                     IHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            453..1511
                     /locus_tag="ACHK0Z_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             453..1511
                     /locus_tag="ACHK0Z_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU4501531.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDVARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKK
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            1552..2274
                     /locus_tag="ACHK0Z_RS07420"
                     /old_locus_tag="pgaptmp_001484"
     CDS             1552..2274
                     /locus_tag="ACHK0Z_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HGU4501532.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSILFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            2271..3092
                     /locus_tag="ACHK0Z_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             2271..3092
                     /locus_tag="ACHK0Z_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGU4501533.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVRGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            3067..3324
                     /locus_tag="ACHK0Z_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             3067..3324
                     /locus_tag="ACHK0Z_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HGU4501534.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            3336..3587
                     /locus_tag="ACHK0Z_RS07435"
                     /old_locus_tag="pgaptmp_001487"
     CDS             3336..3587
                     /locus_tag="ACHK0Z_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4501535.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            3592..4173
                     /locus_tag="ACHK0Z_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     CDS             3592..4173
                     /locus_tag="ACHK0Z_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501536.1"
                     /translation="MSGIRSLPMIKLLTGLLLLVWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTYQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRRKMIKRKTP"
     gene            4170..5528
                     /locus_tag="ACHK0Z_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             4170..5528
                     /locus_tag="ACHK0Z_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HGU4501537.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNSMNKRVYAQLQELFHD
                     TP"
     gene            5515..5868
                     /locus_tag="ACHK0Z_RS07450"
                     /old_locus_tag="pgaptmp_001490"
     CDS             5515..5868
                     /locus_tag="ACHK0Z_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HGU4501538.1"
                     /translation="MIRHEIERHLVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            5859..7535
                     /locus_tag="ACHK0Z_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     CDS             5859..7535
                     /locus_tag="ACHK0Z_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4501539.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKRATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMCHSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FTALLYPVVGVYWLTAARARKASQDWLARVRQQQPQAAKINSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIEGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7539..7961
                     /locus_tag="ACHK0Z_RS07460"
                     /old_locus_tag="pgaptmp_001492"
     CDS             7539..7961
                     /locus_tag="ACHK0Z_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HGU4501540.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            7958..8563
                     /locus_tag="ACHK0Z_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     CDS             7958..8563
                     /locus_tag="ACHK0Z_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HGU4501541.1"
                     /translation="MKFLPLLALLISPFVSALTRDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            8532..10850
                     /locus_tag="ACHK0Z_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     CDS             8532..10850
                     /locus_tag="ACHK0Z_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HGU4501542.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGHYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIASYYPCGIAWVDRKSSF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10847..11431
                     /locus_tag="ACHK0Z_RS07475"
                     /old_locus_tag="pgaptmp_001495"
     CDS             10847..11431
                     /locus_tag="ACHK0Z_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HGU4501543.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWEAQLPAGWTLRDNGDKRELRNTSGKL
                     VTEITYLNRQGKRVPIRIEQHVFKYHITIQYLGD"
     gene            11433..12602
                     /locus_tag="ACHK0Z_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     CDS             11433..12602
                     /locus_tag="ACHK0Z_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HGU4501544.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPNTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFSRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12599..13063
                     /locus_tag="ACHK0Z_RS07485"
                     /old_locus_tag="pgaptmp_001497"
     CDS             12599..13063
                     /locus_tag="ACHK0Z_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HGU4501545.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            13063..13794
                     /locus_tag="ACHK0Z_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             13063..13794
                     /locus_tag="ACHK0Z_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HGU4501546.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13791..15020
                     /locus_tag="ACHK0Z_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             13791..15020
                     /locus_tag="ACHK0Z_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HGU4501547.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(15194..15463)
                     /locus_tag="ACHK0Z_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /pseudo
     CDS             complement(15194..15463)
                     /locus_tag="ACHK0Z_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15860..16891)
                     /locus_tag="ACHK0Z_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             complement(15860..16891)
                     /locus_tag="ACHK0Z_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4501548.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            17162..17605
                     /locus_tag="ACHK0Z_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             17162..17605
                     /locus_tag="ACHK0Z_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4501549.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            17621..17908
                     /locus_tag="ACHK0Z_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             17621..17908
                     /locus_tag="ACHK0Z_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4501550.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            17921..19177
                     /locus_tag="ACHK0Z_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             17921..19177
                     /locus_tag="ACHK0Z_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4501551.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(19477..19677)
                     /locus_tag="ACHK0Z_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /pseudo
     CDS             complement(19477..19677)
                     /locus_tag="ACHK0Z_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20099..21112)
                     /locus_tag="ACHK0Z_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             complement(20099..21112)
                     /locus_tag="ACHK0Z_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGU4501552.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SVEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(21124..22440)
                     /gene="fucP"
                     /locus_tag="ACHK0Z_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             complement(21124..22440)
                     /gene="fucP"
                     /locus_tag="ACHK0Z_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4501553.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(22468..23388)
                     /gene="rbsK"
                     /locus_tag="ACHK0Z_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             complement(22468..23388)
                     /gene="rbsK"
                     /locus_tag="ACHK0Z_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4501554.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            23691..24473
                     /locus_tag="ACHK0Z_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             23691..24473
                     /locus_tag="ACHK0Z_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HGU4501555.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(24475..24701)
                     /locus_tag="ACHK0Z_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /pseudo
     CDS             complement(24475..24701)
                     /locus_tag="ACHK0Z_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(25464..25898)
                     /locus_tag="ACHK0Z_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             complement(25464..25898)
                     /locus_tag="ACHK0Z_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HGU4501556.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRRDVLCCVSCGCPLTLQR
                     GNDGQQPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVLDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVLSEDARADNPLQRL"
     gene            complement(25886..26287)
                     /locus_tag="ACHK0Z_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             complement(25886..26287)
                     /locus_tag="ACHK0Z_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4501557.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            complement(26454..27023)
                     /locus_tag="ACHK0Z_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             complement(26454..27023)
                     /locus_tag="ACHK0Z_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGU4501558.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            27228..27428
                     /locus_tag="ACHK0Z_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             27228..27428
                     /locus_tag="ACHK0Z_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501559.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            28555..28761
                     /locus_tag="ACHK0Z_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             28555..28761
                     /locus_tag="ACHK0Z_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4501560.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            28855..29457
                     /locus_tag="ACHK0Z_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             28855..29457
                     /locus_tag="ACHK0Z_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501561.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            30287..30584
                     /locus_tag="ACHK0Z_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /pseudo
     CDS             30287..30584
                     /locus_tag="ACHK0Z_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            31852..33003
                     /locus_tag="ACHK0Z_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             31852..33003
                     /locus_tag="ACHK0Z_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501562.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            33087..33958
                     /locus_tag="ACHK0Z_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /pseudo
     CDS             33087..33958
                     /locus_tag="ACHK0Z_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            34176..>35874
                     /locus_tag="ACHK0Z_RS07600"
                     /old_locus_tag="pgaptmp_001520"
     CDS             34176..>35874
                     /locus_tag="ACHK0Z_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGU4501563.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     "
BASE COUNT         8922 a         9199 c         9020 g         8733 t
ORIGIN      
        1 gaaacgttat ataaaatggt ttgccatcac aatttttatc agtatgttga gtgcctgtgc
       61 ccgtacggcc ccagtgcaac agataagcac cactgtcagt gtgggtcata ctcaggagca
      121 ggttaaaaat gccattttga aagcaggtgc gcagcgcaag tggattatga cgcaagtgtc
      181 ccctggagtt attaaagctc gctatcaaac acgaaatcac gttgcagagg ttcgtattac
      241 atatacagct acctactata acatcaaata tgacagtagc ctgaatctgc aggcttctga
      301 tggaaaaatt cataaaaact ataaccgctg ggtgcgtaac ctagataaag atatacaggt
      361 taacttatct acaggagcaa cgttatagtg atgctgatat cgggctggaa tggcccgata
      421 gtatttaact ttctctataa gaatggttat atatgtacga acaggacacg ctcagcgcac
      481 tggatgccat caccgaagca cagcggattg cctttgctcc tatgcttttc caggcagctt
      541 tgtgcctgcg caatgcagga atattagatt atttggatca acagggtaaa cagggagctc
      601 cccttaatgc gattactgag cacacagtcc tgaatgaata tgccgtgggt gtgttactgg
      661 atatgggctt aagcgggcga attattacct gtaaagaggg aatttattac ctggcgaaaa
      721 tcggtcatta cctcctgcat gacactatga ctcgcgtgaa tatggatttc acccaggatg
      781 tctgttacca ggggctcttc ttccttgccg attcactcaa tgaaggaaaa ccttccgggt
      841 taaaagtatt tggcgactgg ccgacgatat atccggcact ttcgcaatta ccagacgtcg
      901 cacgtgatag ctggtttgcg tttgatcatt actattcaga tggcgcattt aacgccgcac
      961 tgccttatgt attcgccaac aaccccacaa ccttgtacga tgtgggtggc aatacgggta
     1021 agtgggcttt gcgctgctgc aaattcaatg aaaacatcgc cgttacgtta ttagatctgc
     1081 cacaacaaat cgtactggcg aaaaaaaata tcgcgaatgc aggtttttct gatcgcatag
     1141 atttccatgc ggtggatatg ctaagcgatg ccccattacc aggcgaggct gatatctggt
     1201 ggatgagcca atttttagac tgtttttcac ccgaacaaat aattagcata ctcagcaaaa
     1261 ttgccagcgt aatgaagcct ggcgcaaaac tttgcatcat ggagttgttc tgggatgcac
     1321 agcggtttga agcgacatcg tttagcctga atgcatcctc actttatttt acctgcatgg
     1381 caaacggtaa cagccgtttc tatagtgcag agaaatttta cgactatctg aataaagcag
     1441 gattccaggt agcagagcgt cacgataatt taggcgttgg gcatacactt ttgatatgcc
     1501 aaaagaaata atcgtcactt aataattttg gtttaccgcg ggcgcgcgtt gatgaaattt
     1561 gcattaaata ttaccaactg gcaggcactg gcgcctggac ttagcgatgt gcagcaatgg
     1621 caagcatggt cgcgccagcc gtgggcgatt gaccctgccg cgccgttggc aaaattaagt
     1681 gaactgccaa tgatgaccgc tcgtcgtctc agttcaggca gcaaactggc tgttgaatgc
     1741 ggtctgacaa tgctacgccg ccatcagcca gacgctgttt tgtataccag tcgtcatggc
     1801 gaactggagc gcaattaccg cattgttcat gcactggcaa ccgaacaggc gctttctccg
     1861 acggacttcg cgctttccgt acacaactct tccgtgggta acctgaccat tgtggccaaa
     1921 cagccgatcg tttcgtcatc gctttcggct ggtcgcgaca gtttccagca aggtttgtgt
     1981 gaagtgctta gcctgctaca agcgggatac cagcgcgtgt tgatggtcga ttttgatggc
     2041 tttctacctg agttttatca cccgcaactg cccgcagaaa tgcctacctg gccgtacgcc
     2101 gtcgcgctgg tgattgaagc tggcgatgac tggcaatgtg aaacgcagcc agccattgcg
     2161 gtaaatgaaa caacactgcc gcaaagcatc ttgtttttac agcactattt acaaaatgca
     2221 gatgcttttt cactgcctgg cgagcgcgtg caatggcgct ggagccgcag atgaataagc
     2281 tgatgtcgcg cctgggatgg gcatggcgac tggtcatgac cgggttatgt tttgtcctgt
     2341 ttggtctcgg cggcctgctg ctttcagtcg tgtggtttaa catattgtta gttttggtgt
     2401 gggatacttc ccgccgccgt cgtttggcac gtcgcagcat tgccgccagt tttcgcctgt
     2461 ttttaactgt cgcgaaaggg cttggcgtgc tggattatcg tattgatggc gctgaaattt
     2521 tacgtcagga acgcggttgc ctcgtcgtag ccaatcatcc cacgctgatc gattacgtgc
     2581 tgctggcatc agtcatgcca gaaactgact gcctggtgaa aagcgcgctg ttaaaaaatc
     2641 cttttcttgg cggcgtcgtg cgagcggcag attatttaat caacagcgaa gcagaaaccc
     2701 tgttaccccg ctgccaacag cgactggcac agggtgacac tatcctgatt ttccccgaag
     2761 gcacacgaac gaagcccggt gaaaaaatga cgttacagcg cggtgcagcg aatattgccg
     2821 tgcgctgcac cagcgattta cgcatcgtga cgatcggctg tagcgagcat ctgctggata
     2881 aacaaagtaa gtggtatgac gtcccgcctg ccaggccgtt ttttacggtt gaagtccgtg
     2941 ggcgggtaga aatcaataaa ttttacgatg caacttcaca agagccgacg ctggctgcaa
     3001 gacagttaaa ccggcatctt ttgcttcaat tacagcaagg ttgtttacct ctatcaggaa
     3061 taaataatgc aagcgcttta tctggaaatt aaaaacctca tcatcagcac cctcaatctg
     3121 gacgacttga ctcctgatga tattgatatc aatgcgccac tgtttggcga cggtttaggg
     3181 ctggactcta tcgatgcact ggaactggga ctggcagtaa aaaatgagta cggcatcgtg
     3241 ctctctgcgg aaagcgaaga aatgcgtcag cactttttct ccgtcgccac tctggcctct
     3301 ttcattgctg cgcaacgtgc ctgaggaaaa ccatcatgac agaccaacaa accatttatc
     3361 aggaagtctc agccctgctg gttaagctgt ttgaaatcga tccgcaggac atcaaacctg
     3421 aagcgcgact gtacgaagat ctggaactgg acagcatcga cgccgttgac atgattgtgc
     3481 acctgcaaaa gaaaaccggt aagaaaatca agccggaaga gtttaaagca gtgcgtacag
     3541 tccaggatgt tgtcgaggct gtagaacgcc tgctacaaga aggctaattt gatgtcgggc
     3601 attcgctcgc taccgatgat taagttgctg accggattat tgctgctcgt ctggcctttt
     3661 ttaatctggc taggtttggt gcataacagc ctgcactggc tgttgccgct gatggcgctg
     3721 ttgcttttat tgcgcctgcg ccagccccgt cgccagacgg ggccgctcca ggtcgtcaca
     3781 aaaatcgttg ctgttgtggg cattgtattg tgtgtctcca gttttctgtt gaaaacgtac
     3841 cagttactgc tgttttaccc ggtagtggtt aacgcagtca tgctggcggt ttttggcggt
     3901 tcgctgcggt cgacaatgcc gatagttgag cggctggcac gtttgcagga gccagacctc
     3961 ccggaaaagg ccgtgcgata tacccgccgc gtaacgcagg tctggtgcct gttttttatt
     4021 ttcaatggca gtattgcgtt atttaccacc ttgtatggca acatgccctt gtggacagcc
     4081 tggaacggta tcattgctta ccttctgata ggtttcctga ctgcagggga atggctgata
     4141 cgtcgtaaga tgatcaaacg gaaaacacca tgaatcagac tctctcactt tcacagtggt
     4201 tgacagcctc tcgccctgta acaactcctg ttgcctggct tggggaatat acctggacac
     4261 tggggcatct gcggcatgat gtagcgcttt tgatcgacca tttacgcgac cagccaggca
     4321 atcgctgggc actttgtttt gaaaacagct atctttttat tgtagcgttg ttggccacgc
     4381 tacactcagg taaaatcccg gtaatcccgg gccataatcg cgccgctttg ttaaatgtac
     4441 aacgctcctt gtttgattgc gtcctgagtg ataaaaggct gggctggaac ggtccacagt
     4501 ttgttgtaag cacatctcac cagatgacga ccagcacgat agcctttgat gatattgctt
     4561 ctgacgcgtt tattgaactt ttcacatcgg gttcaaccgg acaaccaaaa caaattgcaa
     4621 aacctgttat cagccttgat caagaagcaa acatgctggc aactcacttt gcagaccgac
     4681 tactgggctg tcgttttgta gcatccgtga tgcctcagca cctgtatggg ctaactttcc
     4741 gtattttttt acccatggca cttggcctgc cattgcatgc cgcaatgctc tggtattctg
     4801 agcaacttgc cgcattaagc catacctacc gctatgcctt tgtcagcagc ccggcgttcc
     4861 ttaagcgact cgaccttcat ctgacgcctc ccccagtgga aatgattctc tctgccggcg
     4921 gtatgctacc atggcaggat gtagagaaaa cttccacctg gctgaatata tggcccgatg
     4981 aaatctatgg tagtaccgaa acaggtattc tcgcatggcg ttaccgtcaa caggataata
     5041 tcccctggtt tactttttct gatgttcatc tttcacagga aagtgagggg gttcgtgtct
     5101 tttcgccact aatccctgct gaaggattgg tgcttgacga tatgttgcaa ttcaatgaaa
     5161 acggtcagtt ccacctgatc ggacgacgtg gtcgagtggt aaaaatagag gagaagcgga
     5221 tatcactggt tgaaatcgaa caacgacttc tggcacttga cggtattatg gatgtcgctg
     5281 caataccatt aatacgcaat ggccgccagg ctatcggtgt cgtggttgta ccaaataaag
     5341 aggcccgtca aatatggcaa tgctccggag ggaaaactct ggagttgtcc tggcgcaaag
     5401 cactattacc atggcttgaa cctgttgcag tacctcgtta ctggcgcatt attgatgaaa
     5461 taccagtaaa cagtatgaac aagcgtgtct atgcgcaatt acaggagtta tttcatgata
     5521 cgccatgaaa ttgagcgcca tctggttcaa ccgcaggaga tggaaatcgt tctatacctt
     5581 gacccgatgc tctactggtt taatggtcat tttgccgtac agcctctgtt accaggcgtg
     5641 gcccagttgg actgggtaat gcattatgct accacgctgc ttgctccggg ctggcgtttt
     5701 cgtagtatcc agaatgttaa atttctggcg ccacttatac ctgagacgac tgttaccctg
     5761 caattaacat ggcaggaaac aagtcaggta ctcactttct gctatcagcg tcacgacggt
     5821 gacgcccgcc acaccgccag tagcgggaag atccgtctat gtcggtaaac ttttctccct
     5881 gcgtgttgat cccctgctac aaccacggcg cgatgatgcc gggcgtgctg gcgcgtctta
     5941 agccatttaa tctgccctgt attgtggtgg atgacggcag cgatgccgcc acgcaacagc
     6001 aactggataa tctggttagc gaacagcctg gcgtgacctt aattcgcctg gcagaaaacg
     6061 caggtaaagg cgcagcggtg atgcgtggct tgcaggcagc ggcagacgca gggttcagcc
     6121 atgcggtgca ggtggatgct gacggtcaac atgcgattga agatatcccc aaactgctgg
     6181 cactcgctga acaacaccct gtggcgctga tctccggtca gccaatttac gatgactcca
     6241 ttccccgctc acggctgtac gggcgctggg tcacccacgt ctgggtatgg atcgaaacgc
     6301 tctccctgca actgaaagac agcatgtgcg gttttcgcgt ttatccggtt gcgccgacgc
     6361 tgcaactggc aaaacgcgcc accatcggca agcggatgga tttcgacacc gaagtgatgg
     6421 tgcgcctcta ctggcaggga aataccagct atttcgtgcc gacccgcgtc acctatccac
     6481 tggacgggct ttcgcatttt gatgccctga aagataacgt ccgcatctcg ctcatgcaca
     6541 cgcgtctgtt tttcggcatg ttgccgcgta ttccttcact gctgatgtgc cactcttcca
     6601 gccactgggc gcggcagagt gaagttaaag ggttatgggg aatgcgcctg atgctgctgg
     6661 tctggcgtct gctgggaaga acggcgttta ccgcgctgct ttacccggtg gttggcgtct
     6721 actggctcac cgctgctcgt gcgcgtaaag cgtcgcaaga ctggctcgcc cgtgtgcgcc
     6781 aacaacaacc acaggcagca aaaatcaaca gctatcagca ctttctacgt ttcggcaacg
     6841 ccatgctcga caaaatcgcc agctggcgcg gcgagctgca actcgggcgt gatgtgctgt
     6901 ttgcgccagg cgcagaagcg gcgcttgacg tccgcgatcc gcgcggcaaa ttgctgctgg
     6961 cctcacatct tggcgatgtg gaagtgtgcc gggcgctggc aaaaattgag ggctacaaaa
     7021 ccattaacgc gctggtgttt agcgaaaacg cccaacgctt taaacagata atgcaggaga
     7081 tggctcccca ggcaggcatt aacctgatgc cggtaacaga tatcggcccg gaaaccgcca
     7141 tcctgctgaa agagaagctg gataacggcg aatgggtggc gattgtcggt gaccgcatcg
     7201 ccgtcacccc gcaacgcggc ggcgactggc gcgtctgctg gagtccgttt atgggccagc
     7261 ctgcgccctt cccacagggg ccgtttattc tcgcctctat tttgcgctgc ccggtgaatc
     7321 tgattttcgc cctacgccag cacggcaagc tgcatattca ctgcgaaacc tttgccgacc
     7381 cgctgctgtt gccgcgtggc gaacgccaac aggcgctgca aaacgctatc gaccactacg
     7441 ccgcgcgtct ggaacattac gcgctccagt cgcccctcga ctggtttaat tttttcgatt
     7501 tctggcaact gccggaaatt caggacaagg agtaaagggt gcttaacgat ccccgattta
     7561 ccgctgaagt cgagctgacc attccctttc acgacgtcga tatgatgggc gtggcctggc
     7621 acggtaacta ttttcgctac tttgaagtgg cccgcgaggc gctgctcaac cagttcaatt
     7681 acggctatcg gcagatgaaa gagtccggtt atttgtggcc agtggtcgat gcgcgggtga
     7741 aatatcgcca cgccctcacc tttgagcaac gcatccgcgt gcgtgcgcat atcgaagagt
     7801 tcgaaaaccg tctgcggatt ggctatcaga ttttcgatgc cgaaacgggc aaacgcgcca
     7861 ccaccggata caccattcag gtcgcagtcg acgagcaaag ccgcgaactg tgctttgtca
     7921 gccccgatat tctgtttgaa cgcatgggag tcaaaccatg aaatttttac cgctgctggc
     7981 gctgctgatt agcccgtttg tgagcgccct gacccgggac gatcttcagc aacgctttac
     8041 cgaacaaccg gtgatccgcg cccatttcga tcaaacccgg acgattaaag atctgccgca
     8101 gccgctgcga tctcagggtc agatgttgat cgcccgcgac caggggttat tgtgggatca
     8161 aacctcaccg ttccccatgc aactattact ggacgataaa cgcatggtgc aggtgatcaa
     8221 cggtcagccg ccgcaaatca tcacggcaga aaacaaccca cagatgttcc agtttaacca
     8281 cctgctgcgc gcgctgttcc aggccgatcg caaagtgctg gaacaaaact tccgcgtcga
     8341 atttgccgac aaaggcgaag gccgctggac gctgcgcctg acgccgacca ccacgccgct
     8401 ggacaaaatt ttcaacacca tcgatctcgc cgggcaaacc tatctggaga gcattcagct
     8461 taatgataaa cagggcgatc gcaccgatat cgctcttacc caacatcaac tgacaccagc
     8521 gcaactgacc gatgacgaac gccaacgctt tgccacccag taaacgcccc gcgctgttat
     8581 gggggctagt ctgcctggtc atggcggcgg cgttgctgat cctgctgcca caatcacgac
     8641 tgaacagtag cgtgctggct atgttaccca aacaggcgat gggcgatatt cctccggcgc
     8701 tgaatgacgg ctttatgcag cgtctggacc gccaactggt gtggctggtc agccccggta
     8761 aagaggctaa tcccagggtg gctcaggagt ggctgacgct gctgcaaaaa tccgctgcgc
     8821 tcggcgacat taaagggcca atggatgccg ccagccagca ggcgtgggga gcgtttttct
     8881 ggcagcaccg caacggcctg attgaccccg acacccgcgc ccgcctgcaa aatggcggcg
     8941 aagcgcaggc gaagtggatc ctctcccagc tttattctgc gttctccggg gtaagcggca
     9001 aggagctgca aaacgatccg ctgatgttaa tgcgcggctc gcagctggcg atggcgaaaa
     9061 atggccagcg tttacgacta atggacggct ggctggtgac gcaggatccc cagggccatt
     9121 actggtatct gctgcacggc gaactggcgg gatcgtcgtt tgatatgcag caaacccacc
     9181 agctcatcac gaccctgaat acgctggaaa aggctctgaa aacgcgttac ccgcaggcgc
     9241 agttgctctc acgcggcacg gtgttttaca gcgattacgc cagccaacag gcgaagcagg
     9301 atatctccac cctgggcgtg gcaacgctgc tgggggtgat attgctgatt gtggcggtgt
     9361 tccgctcttt gcgcccgttg ctgctttgcg tgatttccat cggcatcggc gcgctggcgg
     9421 gaacggccgc cacattgttg attttcggtg aattacacct gatgacgctg gtgatgagca
     9481 tgagcgttat cggcatttcc gctgactaca cgctctatta tctcaccgag cggatggtgc
     9541 acggcaacga cgtttcgccg tggcaaagcc tggcgaaagt acgcaatgcc ctgctgctgg
     9601 cgctgctcac aaccgtggcg gcatatctga ttatgatgct cgcccccttc cccggcattc
     9661 gccagatggc gatttttgcc gccgtcgggt tgagcgcctc ttgtctgacc gtcctgttct
     9721 ggcatccgtg gctgtgccgt ggtctgccgg tgcgcccggt tccggcgatg gcgctgatgc
     9781 tacgctggct ggcagcgtgg cggcgcaata aaaaactgtc gcggggtctg cccgttgcgc
     9841 tggcgctgtt ttcgctggcg gggatgtcaa tgctgcgcgt cgatgacgat atctcgcagt
     9901 tacaggcgct gccgcagcac attctggcgc aggagaaagc cattaccgcc ctgaccggac
     9961 aaagcgtcga tcaaaaatgg tttgtagttt acggcgactc cccacagcaa acattgcggc
    10021 gactggagaa atataccgcc tcacttgagt atgcgaaaaa agaggggctt atcagcaact
    10081 accgcactat tccgctgaac tccctggcgc ggcaggagga agacttagac ctgctgaaaa
    10141 cggctgcccc gacggtaaca aaagcactgc aaaatgcagg gctgacggcg gtgaaaccag
    10201 atctcaacgc catgccggtg aaggtcgatg aatggctggc aagccccgcc agtgaaggct
    10261 ggcgtctgct gtggctgacg ctggaaaacg gcgaaagcgg tgtactggtg ccggttgaag
    10321 gggttaaaag tagcgcattg ttgcaggaaa tcgccagcta ttacccttgc ggcattgcct
    10381 gggttgatcg caaaagctcc tttgatgaat tgttcgcact ttaccgctac gtcttaaccg
    10441 gcttgttgct ggtggcgctg gcagtgattg cctgcggcgc agtggcccgt ctcggctggc
    10501 gcaaagggct tatcagcctg gtgccttcgg tgctttcgct gggctgtggt ctggcggtgc
    10561 tggcgatgag cgggcaggcg gtgaatctct tttcgctgct ggcgctggtg ctggtgcttg
    10621 gcatcggtat caactacacg ctgtttttca gtaatccgcg cggtacaccg ttaacttcgc
    10681 tactggcgat cgcgctggca atgctcacca ccttgctgac gctgggaatg ctggtattca
    10741 gcgccaccca ggccatcagc agttttggca ttgtgctggt gagcggtatt ttcaccgcct
    10801 tcctgctttc gccgctggct atgcccgata aaaagagaac aaaaaaatga tcaaatccac
    10861 attctggcga gcgttcgccc tgaccgctac gcttatcctc accggctgta gccactcgca
    10921 accagaacag gaaggccgcc cgcaggcgtg gctgcaacct ggcacgcgca tcacgctgcc
    10981 ggcaccaggg atttcacccg cagtcaattc gcagcaactg ttgaccggca gcttcaacgg
    11041 caaaacccag tcgctgttag tgatgcttaa cgccgacgat cagaaaatca cccttgccgg
    11101 gctgtcgtcg gtcggcattc gcctgtttct ggtgacctac gatgcgcaag ggctacgcgc
    11161 cgagcaatcc atcgtcgtcc cacagttacc gcccgcaagt caggtactgg ctgacgtgat
    11221 gctcagccac tggccgatta gcgcctggga ggcgcaactt cccgcaggat ggacacttcg
    11281 cgacaacggt gacaaacgcg agctgcgtaa caccagcggc aaactggtca cggaaatcac
    11341 ctatctgaat cgccagggaa aacgcgtgcc aatcagaatt gagcagcatg tctttaaata
    11401 ccacatcacc attcaatact taggtgactg atatgattta tatttccgcc gtcggcatga
    11461 tcaacgccct gggaaataac cttgacgaaa tcgcagccaa cttggctcgc ggtgtcgccc
    11521 ccggcatgcg cccacgcgca ggctggttgc aggggcatcc gcaggcggtc ctggctggcg
    11581 tggatggcga gcttccgctt atcccggaaa aattcgctgc gcatcgctcg cgcaacaatc
    11641 agatcctgct ggcggcgctg gcgcagctcc agccgcaggt tgatgacgct atcgcgaaat
    11701 atggctgcga gcgcatcgcg attgtgcttg gcaccagcac ctccggcctg catgaaggcg
    11761 acacgcacgt taacctgcac acccacgggc agccgaacac cacgtggcac tatgcacaac
    11821 aagaactcgg cgatccgtcg cgttttctca gccactggct ggcgctcgac ggcccggctt
    11881 ataccctttc aaccgcctgc tcctccagtg ccagagcaat gatcagcggg cgcaggctta
    11941 tcgaagcggg gctggtcgat gccgccattg tgggtggcgc agacaccctg agccgaatgc
    12001 cgattaacgg ttttcacagc ctcgaatcgc tctcgccaac cttatgtcag ccgtttagtc
    12061 gtgaccgcgc aggtatcacc attggtgaag gggcggggct aatgctgctg acccgggaac
    12121 cgcagcccat cgcgttgctg ggcgttggtg aatcgagcga cgcttaccat atttcagccc
    12181 cgcatccgca gggcgaagga gcgatccgcg ctattaatca ggcgctgacc gatgcgcagc
    12241 ttacgccgga tgacgtcggc tatatcaacc tgcacggcac cgctacacag ctcaacgacc
    12301 agattgaatc aatggtggtt aacgccctgt ttggcgaacg cgtaccgtgc agctccacca
    12361 aacatttgac cggacacacg ctcggcgcgg ctggcattac cgaggcggca attagtatgt
    12421 tgattttaca gcgtgattta ccgctgcccg ctcaggactt cagcctgtcg ccgcgcgatc
    12481 ccacgctgcc accgtgtggc attatcgaaa aaccgcagcc gctggcacgt ccggtgatcc
    12541 tgtccaattc cttcgccttt ggcggcaata acgccagcat tctgctcggg agggtttcgt
    12601 gagccactat ttatcccccg gcgcttatct gccccacgac gcgcccatgt tactgctgga
    12661 agacgtggtg agcgtcagcg atgacagcgc ggtgtgccgc gtgacggttt cgcccagtgg
    12721 cgtactggca ccgtttctcg acccggacgg caatctccct ggctggtttg cccttgaact
    12781 gatggcgcaa accgtcggcg tctggtctgg ctggcatcgc caccagcagg gcaaaaacag
    12841 tattgagtta gggatgatac tgggcgcgcg tgaattgctt tgcaccactg gcattctgcc
    12901 cgcaggccag acgctgacca ttaccgtcaa actgctgatg caggacgatc gcttcggcag
    12961 ctttgagtgc agcatcaacg acggcgaggc gacgggccgc attaacacct tccagccgac
    13021 cgcggaagaa cttaccaccc tgtttcaaca aggagcgtcc tgatgagtcg ttcagttctg
    13081 gttactggtg ccagcaaagg cattggtcgc gccatcgcct gcaagcttgc ggcagacggc
    13141 tttaacattg gcgttcacta tcaccgtgac gccacaggcg ctcaggaaac gctgaatgcc
    13201 attgtcgcca acgggggtaa cgggcgtttg ctcagtttcg acgtcgcaaa ccgcgaacag
    13261 tgtcgggaag tgctggagca tgagatcgcg caacacggtg cctggtacgg cgtggtcagt
    13321 aacgccggga tcgcccgcga tgccgctttt ccggctttaa gcgatgacga ctgggatgcg
    13381 gtgatccaca ccaacctcga cagtttctac aacgtcattc agccgtgcat tatgccaatg
    13441 atcggcgcgc gccagggtgg gcgcatcatc acgctgtcct cggtttccgg cgtaatgggc
    13501 aatcgcggcc aggtgaatta cagcgcagcc aaagccggaa ttatcggcgc aacaaaagca
    13561 ctggctatcg aactcgcaaa gcgcaaaatc accgtcaatt gcatcgcgcc agggcttatt
    13621 gataccggga tgatcgaaat ggaagaaagc gctctgaaag aggcaatgtc gatgatcccc
    13681 atgaaacgta tgggccaggc ggaagaagtt gccgggcttg ccagttattt aatgtctgat
    13741 attgcgggtt acgtcacccg ccaggttatt tccatcaacg gagggatgtt atgacacgtc
    13801 gcgtagtgat tacaggaatg ggcggcgtga ctgcctttgg tgaaaactgg caggacgttt
    13861 ctgcacgctt actggcgtat gaaaatgcgg tgcgcaaaat gccggagtgg caggtctacg
    13921 atgggctgca caccctgctt ggcgcgcctg ttgatgattt cacgctaccg gagcactaca
    13981 cccgcaagcg tatccgtgcg atgggccgcg tgtcgcaaat gtcgacccgt gccagcgagc
    14041 tggcgttaga gcaggcaggg ttgattggcg atccgatatt aaccagcggc gaaacgggca
    14101 ttgcgtacgg ttcatcgacc ggcagtaccg gtccggtgag cgaatttgcc accatgctga
    14161 ctgaaaagca cacgaacaac atcactggca ccacctatgt gcagatgatg ccgcacacca
    14221 ccgctgttaa tactggcctc tttttcggcc tgcgcgggcg ggtgatccca acctccagcg
    14281 cctgtacctc cggcagccag gcgatcggtt atgcgtggga agccattcgc cacggttatc
    14341 aaacggtgat ggttgccggt ggtgccgaag agttatgccc ttcagaagca gcagtgtttg
    14401 ataccctttt tgccaccagc cagcacaatg atgcgccgaa aaccacacct tcaccgttcg
    14461 atgaaaaccg cgacggactg gtgattggtg aaggcgcagg tacgctcatt ctcgaagaac
    14521 tggagcatgc caaagcgcgc ggcgcgacca tttacggtga aatcgtcggc tttgcgacga
    14581 actgcgacgc ggcacatatt acccagccgc agcgcgaaac catgcagtat tgtatggagc
    14641 aatcgctgaa aatcgcaggg ttaagcgcac aagatatcgg ctatatttca gcgcatggta
    14701 cggcgaccga tcgcggtgat atggcagaaa gtctggcgac agcaaccatt tacggtgata
    14761 atgtgccgct ctcctcgctg aaaagttatt ttggtcatac gcttggtgcc tgtggcgcgc
    14821 tcgaagcctg gatgagtctg caaatgatgc gcgaaggttg gtttgcgcct acgctaaacc
    14881 tgaataaacc agacccgaat tgtggcgcgc tggattacat catgcatgaa gcccgcaagg
    14941 ttgattgtga gtttttgcag agtaacaact ttgcctttgg cggcattaat acatccatca
    15001 ttattaagcg ctggccctga gttatacaac cacatggaca tcccatgtgg tgattcttca
    15061 aaaaatctga atcaatgaat atgaacctca aattctctta cgcatactga agagcattcc
    15121 gtataacgat tcagcagaca aaactttatc agtccacacg aatcaacgga tgcacagatg
    15181 acacgcccgg ttatcaccgg gcaatggttg ccataacggg taacataagg gactgaacag
    15241 ttgtctttag cgcaacgaac tgatctaacg tacctgaacg gacaacctga aaacagcctt
    15301 ccatcctgca ttgtccgcca gtgcttatta ccaaccacgt ctgttgcagt aaagtaatca
    15361 gttgcgaaca ttatttatca atcgtgacgc tggctcgcgc atggcagaaa aggtcctgac
    15421 tgagcaacaa caggcatcgt actgttgctg ctccatgttg tttatatgaa cgcatcccgt
    15481 ttgcaaggct tccgtgtttt tgacgttgac ggtagattgc tgctctatat ccttattgca
    15541 gccggtgctg cgggcctcaa tgagattcgc tgactctcgc cttattcacg caacgagctt
    15601 taagctcaga aaaacatcca agtttggtga gtcacagtct tacctgtcgt tgtcatcaat
    15661 ataggaatgg ataatctgga gagcgatact gcaggctgat attgcggttt tgcgacaagt
    15721 ttttagcatc cgcttctacg ccaaatacct gaagtaggaa tggatgcgtt agcagcatac
    15781 atccggaact gcgcggggaa tagccggaaa cggctatccc taccacaata gctaactttg
    15841 taatagcgct cttttaggct catgtactct gacgtaattc tatctggtag ttgaattgca
    15901 cgatacgcga ctcttctgat tcatcatgat tattattcaa tagtccaatc agtaacttac
    15961 ctgtctctat ccccattttt ttgtagtcaa tacggatgga tgtcaaagtt gggtgtgttt
    16021 gatcgcaatt gtctgaaccg tcaagacaag ctacagcgat atttccaggg atttttaaca
    16081 aacgccgctg gcactcaaac attataccca gcgcaatctc ttcgtggctg caaatcacag
    16141 catccagctc tggttgtcgc agtaatatct cgtttaatgc gtaacgcccc aattgcagac
    16201 ttgcggcatc cggcatggtg ataacaagat cagtgttctg gtaatggctc agcattgctt
    16261 tatgccagcc gttcagttgc tgacgttgca ggcggttgtc catatgcgca ccgataaatc
    16321 cgatattctt ataaccctgc tccagcagat gttcggtcag tttatgtgat gcttcaaaaa
    16381 atgctgtttc gatggtaatt tgagccggac tgaaagggga accgataacg ttaatggtag
    16441 gtatatttgt tttctcgagt atctggtgag ttttatcagc caactgagaa ccaaaaataa
    16501 ctaacgccgc cgggttgcct tgaagaagcg tcatcagaat ttcagcttct ttgagctgat
    16561 tatactcatg acacccaagc agtagttgga attcattttt attaagcacc tgctgtaatg
    16621 actgcataaa gcgggagctg gctttatctg tcagggaagg aaccagtacg gcgactgtat
    16681 gactgtgacc agatgcaagg gcgccagcag ttctgttggg aatataaccc aactcttcta
    16741 cggcctgttc tatcttttcc ctcagcttgt cagaaacctg ttccggtgtt ctcagcgcgc
    16801 gggaaacggt catagttccg acacctgcaa agttagcgac ctcctgcagc gtcacccgtc
    16861 cggtaccacg gcgcttacgt gttttttcca tttcgactcc atggataatt acaaccagct
    16921 gttttcatta cttttatttc accattactc tttccagttt ttgcccacgg tagcaaatat
    16981 cctgttgcat tgtatatgca ggaaccgaat atagccaaag catggcggca gagaaatgat
    17041 agcgctatca caaagattgg tagcgttatc agatagcgct atcaaatgcg acaatcatca
    17101 cattaattaa tgcgtaaaat atctactctc ctgttaatta tcatcgggag ttatcagtag
    17161 catgttaaaa aatctattaa atacagaggt cgttcaggtc gtggaacagg tcaaagactg
    17221 gcgggaagcc gtggcgattt catgccgacc gttgattgaa aacggctcta tcgaaccgcg
    17281 ttatgttgat gcgatctatc actcgcacga cactatcggt ccgtactatg tggttggccc
    17341 tggtattgcg atgcctcatg cgcgcccgga agagggggct aacaagctgt cattagcact
    17401 gaccctgatc ccttcaggtg tgaattttga tgccgatgaa aacgatccag tgaaattact
    17461 gattgtgctg gctgcaaccg acagtaccag ccacattgaa gcgatttctc aactcgccaa
    17521 gttattcgat aacgaaaaag acatacaggc cattttgacg gccaaaacga cgcaggacat
    17581 tttgtctgtt attgcgcgct attaatccag aggaacattt atgaaaatta cagtggtttg
    17641 cggtaacggc ttaggcacca gcctgatgat ggaaatgagc attaaaagca ttttgaagga
    17701 tttgtccgtc agcgctgatg ttgatcacgt cgacctgggc tccgcgaaag ggacgccgag
    17761 tgatatctat gtcggcacca aagacattgc cgagcagtta gccgcgcagt ctgtcgcagg
    17821 caaaatcgtc gcactggaaa atatgattga taagaaagcc atgagagaac gtttgtccgt
    17881 ggtattaacc gaattaggcg cactgtaagc tgggggcgat atggatttct ttcgtttttt
    17941 gatgagcgat gtactttccg aacccgcagt gctggtcggt ttaatcgccc tgattggcct
    18001 gattgcacag aaaaaaccgg taaccgaatg cattaaaggt accgtcaaga ccattatggg
    18061 gtttgtgatt ttaggcgcag gggcgggttt agttgtgtcg tcactgggtg acttcgcaaa
    18121 cattttccag catgcctttg gtattcaggg tgtagtgccg aacaatgaag ccattgtttc
    18181 cgtagcgcag aaaagcttcg ggaaagaaat ggcgatgatt atgttctttg cgatggttat
    18241 caacattatg attgcccgtt tcacaccgtg gaaatttatc ttcctgaccg gtcatcacac
    18301 gttgtttatg tcgatgatgg tagcggtaat tctggcaaca gcaggcatga ccggcattac
    18361 gcttatagcc gttggctctt tggttgttgg cgtggcaatg gtctttttcc cggctattgc
    18421 gcatccgtac atgaagaaag tgaccggttc tgatgacgtt gccatcggcc acttctcaac
    18481 attatcttat gtattggccg gtttcatcgg cagcaagttt ggtaataaag agcactcgac
    18541 tgaagacatg aatgtgccga aaagtctgct gttcttgcgc gacacgccgg ttgctatctc
    18601 ttttaccatg agcataattt tcctggtgac gtgcctgttt gcgggtgcag atgcggtgaa
    18661 agagcttagc ggtggtaaaa actggttcat gttctctatc atgcaatcca ttaccttcgc
    18721 agctggcgtg tacatcattc tgcagggtgt gcgcatggtg attgcggaga ttgtcccggc
    18781 attcaaaggt atttcagaca agctggttcc gaatgccaga cctgctctcg attgcccggt
    18841 cgtcttccct tacgcaccta acgctgtact ggttggtttc ctgagtagct ttgctgcagg
    18901 tttaatcggt atgttcacgt tgtatctgct gaacatgatc gtgattattc ctggtgtggt
    18961 gccgcacttc ttcgtaggtg cagctgcagg ggtattcggt aatgcaacgg gtggacgtcg
    19021 tggtgcgatt ttgggtgcct tcgctcaggg cttgcttatt accttcctgc cagtattctt
    19081 attgccggtg ctgggtgata ttggttttgc taacaccacc ttcagtgatg ctgactttgg
    19141 cgcactgggt attttgttag ggattatcgt tcgctaatat tttgatgaca ttaatatcct
    19201 ccaggatttc aggaacgtgg aggatattaa ttcctcttat tttctccatt tttctttctt
    19261 ccagtcagga ctgcatccga ttaatcgtaa gcgctccgag aagtacgtag cgtaaggatt
    19321 attttacagc tgagaagttt cagtgcagca gttcatgcac acggttcgac ggctagtcat
    19381 tgatcttcat aattacctcg cgtaaaaaac ggggtaaggc cgctcatcca gcgtcatctc
    19441 cacaggtaat aattcaacgc caatgccaga agagttcgtt accggaagac gccgtgatat
    19501 acgccctttg atcagcgaga gcctgagcca tttgaacaga agattatcat tgatatcatg
    19561 ctcccgggca atacgggcaa cagaacccct tggttgtaaa gccagtttaa ccatttgaag
    19621 tttaaactca tttgaaaatg acctgcgggg ttcaactgat aatgtttttt gttccataac
    19681 aggtgtccac tagttgaaaa ggtgggtacc tatgttacca atactggctt aatggctaca
    19741 tacagcagtc ggtttacgca tacactttta tagtgcactc cggcctcatg aatataacgg
    19801 tggattaccg ccgaacgaat cggaaaatag atactggaaa aaaactgaaa ttgtgaccag
    19861 tttttgttga ccactacaaa taaataaaag agttgcagtg cttgctcggt aagaaaacaa
    19921 tggaggttaa gctcctcaaa gaagctgtgg agtacaccac tctacagtag ttgcgcaaat
    19981 ttggagtgga cgataatgtg atcgtctata agggcaacgc tatcatagtc ttgtcctggc
    20041 gggtaaaaaa acgcgcttac cttaacgata agcgcgccgc tgttcaggcc ttgagtggtt
    20101 attcaattcc tgtggtgact gtaaaagtgc gcgtttgctg cggtgcaacc tgaatcagcg
    20161 tgccattacg ttgcgcggca agatacccct caggccgaca ggttgcaggt aatgcaaagg
    20221 cggctacctg ttgctctccg ttataaagga tccagcgtgt cacataattt agttcagcac
    20281 tgtagaaacg agtaacaaac gtagtgccat cgggagagat catgcgaaac tctggctgat
    20341 ctgtataagc gtccagtttg tctgcaaaga agacaatttc tggatcataa aattccggtt
    20401 gactcagcgt cgacagagag gcttctccct gcataatccg ttgattaaac gccagccact
    20461 gagcggtggg attaacatgc gaaggcactg attcacgcaa tcttaatatt tcgtccggga
    20521 tattctggct gaatgtagca tttggtatat atgcataatt catgtggcac atatattgta
    20581 gtggcatatc tacagaagcc agattggtta cggccatctt aatatcgaac agtgtagagg
    20641 atttgtgaag gaccactgtt ggctgagcca gataatgatg accgaaaccc attacatact
    20701 cgtaacgccc gttaaggcgt aacatatctc cgtctaattc cagccatgct tcatccatcg
    20761 cggcacaggc catttcaccg tgtagcagat gagtatcttc cacagatggg cagccattag
    20821 ccagcaaacc tgaatgaaaa gcaaaacagc cataggtctc tatcacctct gtcgccggtt
    20881 taggctggcg aaacatattg cacatggtga ggccgtgtcc atcaaattgc gcatcccaaa
    20941 tcatctgccc catccaggga agaataatca aatgtccacg actgtttgca attttaagcc
    21001 cctcgacacc gctgtcatat cgaaaagacg tgacagtaaa atcactattt tccagcaaga
    21061 tacgaggttt ctcgccaaaa agcgcccgcc acaaattaat acgcgtactc ataacgattc
    21121 tcctcaggac gctgtgactt cagccagtgc ggtacgtact ttgctttcac gccagaagta
    21181 gactccgaca tagacaaagc agagcataga aaccaggaat gaaagctgta gtgagtggaa
    21241 catatctgca atatatccct gaattgccgg aaccaccgcg gcaccgacaa tagccataac
    21301 aatgactgct cctgccattt ctgtatgttc gttatcaact gtatccagtg ttcctgcata
    21361 gatcgtcgcc cagcaagggc caaacaaaac acttaccagg acggcgacat agaccgcgct
    21421 gaaacttgga gccagtgcaa catatgccag gaacagcgcc cctataacgg aatagagaat
    21481 caatactttt tccggattaa aacgcgtcat aaggatattg gctataaact tgccaataaa
    21541 gaagcaggca aagctataga ccatgaagtt tgaagcatca cgttcgttga tatcgcccaa
    21601 ctccagcgcc agacggatgg taaatgacca tactgcgacc tgcataccca cataaaggaa
    21661 ctgcgccaca ataccgcgac gaaagcgcgg atttctagcc agatagcgca gcgtatccat
    21721 tgctgacggg cgtttatggt gacttgtctg tgccacttta caggttggga agcgggttaa
    21781 aaggaacaac accatgacca caaccagaat cataatcata tacttatacg gttcaagggt
    21841 gttctctaac atcagcacct taaagttgtg aatttgctcg gcgttcattc ctgacatctg
    21901 cttttcaagg ctttccccct cggagaaaac cagatatttg cccaataaaa taccagacgc
    21961 agcaccaatc ggataaaagg tctggctgat attgagccgc aatgtggcat aggcttttgg
    22021 accgatcatt gaactgtatg tgttcgctgc agtttcaagg aaactcaggc caatcgcaat
    22081 cgcaaaaata gctgcaagga acatagtgta ggttgccata tgcgaggcag ggaaaaaaag
    22141 tgtacaacca ccaatataca gcgtcaggcc aattaaaatt gccaccttat aactggtctt
    22201 tttaatcaca agggatgctg gtattgcaat taaaaaataa cctccataaa atgcgctctg
    22261 caccaatgct gaagcaaagt tacttagcga aaatacactt ttgaattgag tgattaatat
    22321 gtcatttaat gcagctgcgc atccccatag cgggaataaa cacgataaca aaataaactg
    22381 gaacaaggga gtcttattca gatacccatc aggcatctga atgatgtttt tatcgttcat
    22441 agtgctacct ttaactgtgc aggatgatta ttcgtttaag gttaaaaatt cattaaattg
    22501 ttcaatactc ggataagatg attgcgtacc tttccctgtg acgctgaaag cggcaaagag
    22561 agcggctttt ttcaaagcgg cttcaacatc accgctttga acataataat gggaaaagca
    22621 accaataaat gcgtcaccag cgccactagt atcaacagca tttactttga atgcaggaac
    22681 atggacttcc tgatcgcggg tcatccataa tgcgcctttt tcgctcatgg taacaataat
    22741 attgttcagc cctttatcaa ctaacgaacg tgcggccaaa cgaatatgat cataagtatc
    22801 aaccgacata ccggttaata tttccagttc tgtttcattc gggataaaga aatcacattt
    22861 gcaggcataa gacatatcta actcacgcaa tgccggagcc ggatttaata acacttcaat
    22921 accatttttc ttaccaaact caatcgcgtg gtaaactgtt tccagttgaa cttccagttg
    22981 taaaacgatc aatttgcatt ttttcagatc ttctgcagct cgatcgatat cttccgggga
    23041 aagaaattta ttcgctccct taattattaa tatactattg ctcgagttgg cattaacaaa
    23101 gatcggtgca acaccactgc tggtacaggg gactttctca acataagtgg tattaattcc
    23161 ccatgattcg agattacgaa tagtattatc cgcaaaaata tcatcaccta ctttagtcag
    23221 catcaggact tttgaattca atttagccgc cgccaccgct tgattagcac ctttcccacc
    23281 acatccgatt ttgaaggcag gtgcttccag agtttctcct tctttaggca tctgattagt
    23341 gtaagtaatg agatccacca tattggaacc aataactgca atgtccattt cactacctct
    23401 tataaacttt cgcataacaa tggtatttaa ataacattaa catgttactt ttgcatcatt
    23461 tgtgactgag atcgcgatta gcacatcaac ccgatgttta tttaatagac ttccagtctt
    23521 atcactcagg ccaacactat ctaatcataa gcaacctaac aggattaata ccgaaaattc
    23581 agcagtctat acccttttca tttcaaaggg tcggtcgtat agtatggtaa ctaaaacaat
    23641 gtttactaat gccataatgt tatttttata acattttacg gagagagttg atggaaacga
    23701 agcaaaaaga gcgtatccga cgtttgattg aaatacttaa gaaaaccgac agaatccatt
    23761 tgaaagacgc ggcacgaatg ctggaagttt ctgtaatgac tattcgtcgc gatctccatc
    23821 aggaagatga acctctgcca ctgaccctac tgggtggcta tattgtaatg gtgcataaac
    23881 ccgcaccatc catgccagta atccaggacg ttccgagaaa tcatcgtgat gacttaccta
    23941 ttgcaattct ggccgccgga atggttaatg aaaatgatct gatcttcttt gataatggcc
    24001 aggagatacc gctcgttata agcatgatcc cggatgcaat caccttcact ggcatctgtt
    24061 actcacatcg tgtctttgtt gcgttgaatg aaaaacctaa tgtgacagca atactttgtg
    24121 gtggtacgta tcgtgccaga agtgatgctt tttacgatgc cagtaactct tcgccattag
    24181 actctctcaa tccgcgaaaa atatttattt ccgccagcgg tgtacatgat cactttggcg
    24241 tcagctggtt taatcccgaa gatcttgcca ctaagcgtaa agcgatggcc cgtggactaa
    24301 ggaaaatttt gctcgcccgc cacgccttgt tcgatgaagt agcctctgcc agcctcgcac
    24361 cgctctctgc atttgatgtt ctgattagcg agcgtccgtt accggcagat tatgttacgc
    24421 actgccggaa tgcttctgta aagatcatta cacctgattc agaagacgaa tgacttactg
    24481 aaaaaacacc gtactcttgt taaacatcgt cggattggac tgattacgtt gcactttcat
    24541 cacatattcc agtttatcaa tttggcttat catctactcc agacgctggt catcattgac
    24601 caatagtctg atatggcttt tgtcgctgtc ctgaatcggc aggcagaaaa tgccttcaac
    24661 gttaaaagca cgacgggaaa aagcccacaa aggttagtca ttacgcccgg ataataaaca
    24721 ggctcctccc tgttatggaa gccgttgtat tgtccataat gtctgggttt ttaatgcaca
    24781 ctggataaca accggacgaa tcttcaaata ttactggcgc tgacagccgg tttgaattca
    24841 tctcacaacc ctgcatagag cgaatctcct gcctgcacgt cactccactc catggtatca
    24901 acttcacact ctttatctgc ggctagtttc agtcaccaga taagcatcga ctatgaaagg
    24961 tgagccatga caacataacg ttggtaacgc tctgacgcct taacggaaga tgcctgccac
    25021 cacagggaat gtaaacgact gaagtgtggc ctttaatgcc gtgaacggct catggtctcc
    25081 tggcacggtt gccgccccaa cctgtaacaa cattccacag tacaatgtct gtcagagccg
    25141 gagcctccca tgcctgttga ataaaacccc ggatgcttcc cccgattctg caagtaatac
    25201 ttcggttatt tacactattt tatccctgtt gaatttggtt aagccggtgc agacgcgccg
    25261 ggaaacgcac gcctgaaccg gcacaataaa atcctgttac tggagacacg aatggaatga
    25321 cgaggcagcc tgtcagtcac aggggggctg ttggcataaa atcaacggaa aggccaccaa
    25381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa cggcatcacc
    25441 gcaaactgcg ccagaggcaa ccattacaac ctctgtaacg gattatcagc cctggcatct
    25501 tccgacagca cctcctgccg cccccacgcc ctgctgtata ccccggtacc acaacgcggg
    25561 cagcactttt ctccttcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
    25621 accgtataat ccagaaccgt cataccctgc agatgcaaac gacgggcctc tttctctgcc
    25681 gggtcaagcc aggtgcatcg cagcaggatt ttctcagcga cagtcatctg gtcatgttca
    25741 aaccacggtt gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
    25801 cagcacaaaa cgtcccggcg gttgtgcttc gctgcttctt tcgccgtaac aaaatgcccc
    25861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
    25921 gaatactgaa tcctccggtc tggcagtttg tgcagtactg ctccccataa taatcgtggt
    25981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
    26041 gctgcagacg cttaatcaag cgtacctccc tgcgttccgg tctgacataa gggcactcat
    26101 gaccgtgctt agtcagcccg tcgtcagtgt gttcaaacca gggacgttca gtgtcgtatt
    26161 gcgggtggta tctgagtgca ctgccgcaaa ggtggcaagt gtagcgatcg taaggtgcag
    26221 tctgtgcggt acgggcacca gtcagacgcc cgttgccatc aagtgcgaga aaggattttg
    26281 cgtacatcgt catattcctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
    26341 ggcagcgtgg tcaggtgtga atagcggttt tcagttcagt ctcagaaggg aaaatcagtg
    26401 tggtcgctgt gaacgcgtca tcacactaag gtcagataag tcgtctgtcg ggctcagcca
    26461 cgactgcagc caaagttgcg ctcaccggtg ccgcgagctt tggtatgctc cttcgccaga
    26521 tagccagcac gttccagcac ctgctgaaag ccagtgtcat catcgcgttc cagccacacc
    26581 gccggccagg ccggaaaatg caccagcgtg gcatggcacc cgacatccac tcccagggca
    26641 ctgcaccacg cctgtttaat cattccggcc agtgaccccg gagcgcggta atcgccggca
    26701 cgacaccagg tatcccggtt gaccagcagc agcaggtgat agtgtttttt gcctttaagc
    26761 tccccgaact cccgggccca ggcgtaatgc agggtcgagg aatgcacgcg tttaccttcc
    26821 cgatgcttac gtttctggta agcgtcgatt cgggctttca gggcattgat gaagcgggat
    26881 atcacagccg cgtccgttgc tgccggtaca tccgggagac gcagatcaac ccgcaatgcc
    26941 gtcagacggg gatgaacatc cagtgcgtgc cgcaccgtct cacgaatacg ttgctgccag
    27001 aaggggttgt atttgtaggt catggttaaa tctccgtatg gtttcgttca tacggattaa
    27061 ccacgccgta aaaaatgcgc agagtccctg aagtgatctc caaccaggca atatctcagg
    27121 cactctgaga taacccgaac ggtcgtctcc ggactgacgg ttgaattttc agcgttgttt
    27181 ttttaatgta aaaaaccggt tacgggtaag gctgtgagga gggagttatg gtgatacgca
    27241 aaaagaagtg ctgggactgt ggaaacgcga ttactcataa tactgtgtgt tgcccgtatt
    27301 gtggttctgt cgaccccttc ggctattacc ggaatacaga ccgtatcgtt accatactgc
    27361 tggcactgat tattgtggtc ctgctgacta ctgtcagcgt cagtgtgtat atactgtgta
    27421 gctggtgaga taacggttca tgtatcccct gagctgacag tgtaccgggt atattaagcg
    27481 taaccgacag gttctccttc ataccatatt aaatttttcc aggtattgcc gctgccttat
    27541 ttcgtcccat acaggaaaac tcgtctgggt ttctgacaaa tacgcgaaac tcacagcccc
    27601 tcaatatcaa ccacacgctg taagccggta atacctgaaa atcagcctat taccgcccgc
    27661 gcctccgtta ccgaacgctc aggtgaccag actgaagccc agcacgcact cgtaaaattt
    27721 gcggtcagtt ccgtcagtca gtgggcaacc gcagagttac gggcttccag gtccacagga
    27781 gtgagtctat aacgcgtgtg ggaaatcatt ttttctggcg ttgacagaac gccttacggg
    27841 ctgctactta cccggtgtcc cgcaatacag ccttagtgtt ccgttatcgg ggatccggac
    27901 gcaccgcaac aaccggggaa gtatttctgg tcagagctgt gtcaagaggg atttctcagc
    27961 ccctcactca ccggctcagt ctcagggcag aagcattaat acactcagtt catgtcttat
    28021 acaggaatgg gcataggggg agctggcgtg tcactgctga ttttacgcat cagcgttcat
    28081 gtatctgcac tctgatacag aatgcttcac agcgctctgt taccggagag atttactctc
    28141 ttaggttatt ctggcaaatc ctttacgtta ctctctgatg acttcttata tttataatac
    28201 caatcagaca accttatccc atcggtgata tcagtctcct gctcgccagg aacgggctga
    28261 ctcactgtaa ccacctccga aaacaactct gcaattatca gaacgcctgc ttctctccct
    28321 gtcctcacga aaactatccc ctctttatcg cgcgtgcgtg cggaagcatc ttttcgcaac
    28381 acccccctcc gggattccgc tacggctctg ccatcgcaat ccccccgttt atctccggac
    28441 agccgcattc ccgattattt tttacgtttc tccccggttg ttatgccggt gaaggtggcg
    28501 cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat tctcatggct
    28561 accccagttt cgctgatgga tgaccagatg gtcgacatgg cgtttatcac tcaactgacc
    28621 ggcctaaccg ataagtggtt tgacaaactc atcaaagatg ggggctttcc tgcgcccatc
    28681 aaaatggggc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct gcaggcgcgt
    28741 attgcacagt cccgtccgta atttttaacc actaaccctt cgcccacagc agacgcaatc
    28801 tccggcctgc cggcggcgct ctgctgcctg ttatcccccg tgaggaatat gaaaatgaaa
    28861 caacagtatc agacccgtta cgagctgctc catgaaaact accagaaatg gctgaccggc
    28921 ttcacccggc acgccgtatc atggggcgtg tgtcatccga atatctacta tttccataat
    28981 ctgacgcccg ggtgggtgtc attcaatggc gaaaaaccgg agattgccat cgttccccag
    29041 agtctgcacc ggctgattta tggtccggac aaacggagct ccccgtccct ggatgatgat
    29101 ctggtggtga atttatgcac cagcgaacac ctgctggttc atcatccgat gctggaaggg
    29161 atcctgctgt ctgaatgtga gcgcctgaag caacattctc tggcaaacaa actgatcagc
    29221 ctcttccgtc agtttggcgg cacggagtta cgtctcaaac tggtctggct ctgctggctt
    29281 gacttaatga ccggaaacag ccttgacgac tggacgaaaa acctgaaaca caaatcagaa
    29341 aaagatctgg agcaatggat cattgcacgt cagggtcaga gtgaaccact gacaaatctg
    29401 atggatcagt acgtgcttat ggcataccgt acatcggttg atgccgctca cagttgatgt
    29461 ctgagaacca cagggctggc agcagaaaac cagccctgtg gtaaaataca gtaatagcgg
    29521 cgtacatcag gcacgccgct cactcgtcca cagatcatat acgtcattat actgcgcgat
    29581 aacggagggc ttcagcaccc ggtcatttct gagcgggtat gaataccatt gatgatgcga
    29641 agcagccacg ggcagaaacg ttcttcacaa ccaggtgcct ccatcttcac tgcagtctga
    29701 atgtgccggc ttgttatcca ccagaaatgg tatgctccgg tacatgcata ccccggagac
    29761 tataaacgca ctgaatcata gcctcccacc tcagcaggag gcagtcagct ttcaggcgga
    29821 aaaacgttgc agttaaagat cgtgagaatc gctgtttcgt tgcatagcaa ctcgaacagt
    29881 tgtagtgaaa cattttacct gcatcagcac atggtcaggc gccgtctgta tggcgtggaa
    29941 tactgatgtt tcggctgatg gcaagcctca ttctctgtgg aaacaggggc gcgcattagc
    30001 ttctcaacca gagagtgtca caaagacaaa tattgctgaa aatcgcattc atcgttcagg
    30061 cgtgaatgcc cggagacaaa agccgctgtc cacacagtcc ccctctgacc tgaacaaaaa
    30121 gcatacacac aacaggctca cctgaccccg aaaaaccata acttcagcaa taccagtgac
    30181 accagcacgt tacctcagtg ccacatcttc agaccgcgat caatctgaaa tgtgactatc
    30241 ggggcaatat ccctgctttg tggtacacga taaccagaaa acatcccctg aaccgccagc
    30301 cagaaagact ggccaacaaa aggggtaagc tgaaatcgtt tatggattac atcgacatat
    30361 gcctggcaga gaatgtccgg aatgacgagg ttatctttgc ggagattaag gcaatggtat
    30421 acgggccgac gttccatgcc gcgttactac attcagcccg gacgtaaaat gcgcccgtca
    30481 aaaagaacgg ttcgcttcga aactcagcca ggataccacc tctccgccac acccgggaag
    30541 aagttgatat ggtggttgcc ggacaacagc gctcctggat tgactgctgc atcactcaac
    30601 cacgctgaat atcaaaggag agcgttaccg gttaaaagag aaacgtaaag ctgtggtgat
    30661 gacccaaaac gcaacgcctg tcagtgagga tgaaatggtg aaaagcggac agcatcagta
    30721 acggaagtat cttagcgggt acgaaaatgg caggatcgtg acattgacag aatatctcct
    30781 gatcatcctg aatgactgca gttgctgtag aaaagtaagc tgtctccacc cacggcacca
    30841 cttaacaggc attccgtaac ttcccccaga cttactatga cgcattatcg gtgcactgaa
    30901 aacgggagag tgcgcttaaa accgcagacc gcatgcgggt gttactgcat gacagtctgg
    30961 ccgtattgtg agatgtcggc aggaaccgat gtccgcgcag gggggaactt cctgcagtac
    31021 ctgacgcttc aaatggtatt aaccgtaggg tgttgttggt tccggatcgc ggaaatggga
    31081 gcacaggtct gctacggggt gtacctctga cagtatatcc ggctcgctca tctgacagcc
    31141 tctctcccgg aaaagaccaa aaatgagatc gtctcctcaa cagcagagaa gaggtcttga
    31201 cagacaggct acattgcata cgatttatga agaaaaagac agcaaatatg gaggttatag
    31261 cggagaatac cctacgaaat tcctatgaaa aacgattgaa aaaaatatca aattcgattc
    31321 gtttttatat gctttttggg agcgtggcga tgacgaactg ttgcatgtgc agaggagaat
    31381 gcacccggaa gcaccagaat catccagaaa ggcagaacga ttacgcatca aaccaggaga
    31441 cagcaatatc aaggtaatat actgatttaa aatgaattta cagcaatacg taaaaacatt
    31501 ttttacttaa aggagacaga tgcgctgaag agagtgcagg tatttttata taaaaatgag
    31561 aagtttacgg gatgtgtatc cggcacacta tgaccggaga atatcagtca agcttaaaaa
    31621 gacaataaat acagtgagtt aaatacacag agaaaacgta gtatcagagg gtacatctta
    31681 caacagagac tgaatttccg gtttttcata accggcgcat tccggaaagt gcaggcggcc
    31741 cggaggtttt ctgacgccac tacctggtag attgttctgt acatcatgta cggaggtttt
    31801 tgtttgcttt tttaaaatct gcaaatgtga gaaaagcagg tgaggtgtat tgtgggaaag
    31861 gaactggatg atatctgcac cagctgtccg tatatcgatg ctgtaaaacg gcacaaacaa
    31921 cagctcggtg ccatagagga atatacacag tggttaaaaa aagaaccacg ggcatcttat
    31981 ttttttctgt tccagctgta tacccgcatt cacaatacat ttttctccca aaagcaacaa
    32041 ctcccgttca ctcccggtgg cactcactgc ccggaacctg atgtcaccct tcgggactta
    32101 acgctttccc ctggctacca cagtgattat gcccctcaac ccattccgga gatggacagt
    32161 tctgctgttg ttcccccaac taatgaaaac acctcgcctc cggaagatac tcccgacaac
    32221 actccggccg gaggcaatac cggacaagcg gaaaagacca gaaacgcagg actcacaccc
    32281 attcctgaaa aacgttcagg aatgcccccg gaacatctgc ggtttgccac ggggtttcca
    32341 tcgcaaccga agattgccgg tcccagagaa aagccgatgc gcaccgtgca tccggataaa
    32401 atctaccgtg aaattatatg gttctgctcc agttacctgc tgaaatccgg accagaggct
    32461 acgaggacga ttatcaactc agtattctct gaatgggcca gcataaacaa tgattacccc
    32521 tccccctttt cgtgggtgga cagcagggac agtgaacagt gtgactggtt atggaacgcc
    32581 atgcaggtcc ggtgtgtggg aaccccgctg aatcccctta ccccggagca gaaatactgg
    32641 tttgcctgcg ccacgtttga taactgggag ggctggaatg agcaacaggt acagttttta
    32701 ctggaaagta atcccagacg gaacagagcg aagtttacgc aggtctcctt ccaggccccc
    32761 aggattcagc ataaagcgat tcttcttgat gagctgaaaa gtgcccggga gcaacaaaaa
    32821 aggcgtgatg aacgtgctga tggttccgtc cctctgaaac tgtccggaaa aatccacaaa
    32881 cagcttgaaa gtattgcccg aagtcgtggt gtcctcccaa aaaaactgct gaatgaaatg
    32941 attgagcagg cgtaccatga tttggtggca accaggcaga acagccaaat cgattccaga
    33001 taatttcaga cagatattat ttccgtgaat cccccgccac ctttccggtg cgcggggttt
    33061 tgtctttttt caccgggaat acatgtatga atccgtctga tgccattgag gcaattgaaa
    33121 aaccgctctc ctccctgcct tactcacttt cccgtcacat cctggaacat ctgcgcaaac
    33181 tcaccagtca cgaacccgtg cctggcatta tgggtaaaag cgggcccgta aatcctcact
    33241 ctgtaatgcg ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg
    33301 ggaagtgcag cgcttccgtc tgaatggcca tggtcacagc atggttatca ctgacctgcc
    33361 cggggtgggc gagagccggg acagggatgc agagtatgaa gccctgtacc gtgacattct
    33421 gctcgaactg gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga
    33481 tgagtatttc tggcgacaca tcctgcaccg gggacatcag cgggtactgt ttgtggtgat
    33541 gcaggccgat aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc
    33601 cgcagaggca cagaacattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt
    33661 acatcgggtt gttgccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc
    33721 gctcatgaca gcacttcccg accatgccgc cagtcccctg atgacccgcc tgcaggacga
    33781 gctgcgcacg gagtctgtcc ggtctcaggc ccgtgaacag tttaccggtg cggtggaccg
    33841 gatatttgac acggcggaga gcgtctgcat tgcctctgtt gcacgcacgg ttctgcgcgc
    33901 cgtccgtgac tcggtggtct ctgttgcccg cgcggtatgg aactggattt tcttctgaac
    33961 ccggtggtga tgtaccaggc tgttgttaca gatttttaac tccatgaaaa cactatgcaa
    34021 acgcagccat acccgggttg tccccttgcg ttgtgtttct caagttaata gaatgcatcg
    34081 atcgttacta tcatatcgat cgttataaac gatcgtttgt agtaaatgac ctcctccgtg
    34141 gttgtcatga aaatgcgtct taggaaggtt aaataatgca cgaaaagaac atcgccctgc
    34201 tttgtgatga agccgaccga cttttgcaac tgaacattaa tctgctccgg caaatggttg
    34261 aggagccaga tgtgttatct gacagtaaga acgaaaacag actgcttttt gataaacaga
    34321 aagcactgaa aagaattgag gagctggagg gcgaacaaat caaaaccgcc cgcagggaga
    34381 tggtgctggc tgttgtcggc acgatgaaag caggcaaatc aaccaccatc aacgccattg
    34441 tggggcagga aattctgcct aaccgtaacc gccccatgac ctctgtaccg acgctcatcc
    34501 gccacgttcc cggaaaaact gagccggttc tccatctgga acatattcag cctgtccgca
    34561 atttattaat cacactgcag gaaaaactcg ccaccccggc aggacagcag gtcgcacaga
    34621 ccctgcagca aaccggggat acccgcgaac tgctggatat tctgacggat gatggctggc
    34681 tcaaaaatga ataccacggg gaggaggaaa tctttaccgg actggcatcg ttaaacgatc
    34741 tggttcgtct tgctgcggca atggggactg aatttccttt tgatgaatac gcagaagtgc
    34801 agaaactgcc ggtgatcgac gtggaattca gccatctggt ggggatggat gcatgccagg
    34861 gaacactcac actgctggat acccccggcc ctaatgaggc cggacaaccg cagatggaag
    34921 tgatgatgcg ggatcaactg cagaaagcct ctgcggttct ggctgtgatg gattacaccc
    34981 agatgaactc aaaagcggat gaagacgtcc gtaaagagct taatgccatt gctgacgtat
    35041 cagccggccg cctgtttgta ctggtcaata aatttgatga gaaagaccgc aatggcgatg
    35101 gggcagatgc cgtacgccag aaagttccgg caatgctgaa cagcgatgtg ctgcccgcct
    35161 cccgcgttta tcccggatcc tcacgccagg catacctggc taaccgtgcg cttcatgagt
    35221 tacggaaaaa cggaaccctt cctgttgatg aagcctgggt cgatgatttt atcagaggtg
    35281 ccttcggttg catgaaaaaa gaatacgtct gtaaagacag tgaactggca actgaagggg
    35341 cgacagacct gtgggaaggc tcacttatcg atcaactgat aacggaagtc atacagagct
    35401 cacattccag agctgcggca ctggcggttg actctgccgc cgcaaaactg atgcagaatg
    35461 cagaaaatat cagtgaatac ctgttgttac gccatcaggg gctacagcaa agcattcagt
    35521 cactgcagtc gcatatcacc agcctgcttg cggatatccg ggaaatcgcg gactgtcagg
    35581 agcagatgac cactgatgtc agaatggcca tggaggagat cgatactaaa acccgggaat
    35641 tactgacggg ggtctgcacc tcactggaag aggagctgaa tgactatttc agaagcggta
    35701 aacgcaaaga acagcaaatg ctggaggaag aagacgcaga acaacgaagg tctcagtccg
    35761 gattatgggg gaaaatttct caatggtccg gtatcaacaa ccagggccgg gaggattaca
    35821 ggaaacgaga ttttgccccg gacagccccg aaataaaatt cagtgatcgc aggg
//
LOCUS       DAZFWP020000034        35783 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFWP020000034 DAZFWP020000000
VERSION     DAZFWP020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.034"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(115..2106)
                     /gene="tkt"
                     /locus_tag="ACHK0Z_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             complement(115..2106)
                     /gene="tkt"
                     /locus_tag="ACHK0Z_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4501564.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2384..3142
                     /gene="loiP"
                     /locus_tag="ACHK0Z_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /gene_synonym="yggG"
     CDS             2384..3142
                     /gene="loiP"
                     /locus_tag="ACHK0Z_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU4501565.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3228..4790)
                     /locus_tag="ACHK0Z_RS07615"
                     /old_locus_tag="pgaptmp_001523"
     CDS             complement(3228..4790)
                     /locus_tag="ACHK0Z_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4501566.1"
                     /translation="MERTLPWKPLLCLLFLLSTGCDRAEKQKIAAQLQQDKEAISSVV
                     KKSYRALCGPEFVTSTDSHDRLAEMQQVFTPALMEPVLKYWQESPKTCKRRFPSYSDP
                     LATFVEGDKAQIGAFFGHQSYIDIPMELIREGDGKWKIAAIDFASLHEFEKNGRVTDE
                     VQKKIDLLKKRSFKQHRDFPLLTRTAPERVSNSPLTSVEQTVTEPDSSWLDVLLTIGK
                     YALYLVAIILILALVAVLWIGYGLVFAGHRLFKASCKDPSIQKIPAAERWAMAVGAPY
                     AIAGNNHWARRVVNDDSVEAENDCKSEVESLADMWGVFDRDSLLEQLLALFVAGHRSV
                     YAEQIKNDSEMPDAEYRAFAQQLAQNAKSSSEAKERLWQLQMVRKNRRKICDIDFCAW
                     DMVRFVMLCNSGAQVGYITQREMVDFSMLAARRVQQHYRSWRELAGHFLLARWYWKAT
                     DKRHLITHILFKKAITSLLKEQGSPWRTLPWDTPLPEGTETAFAWACGVSTADVVEVQ
                     PDTPDKAIYEAN"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="ACHK0Z_RS07620"
                     /old_locus_tag="pgaptmp_001524"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="ACHK0Z_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU4501567.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5998..7974)
                     /gene="speA"
                     /locus_tag="ACHK0Z_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             complement(5998..7974)
                     /gene="speA"
                     /locus_tag="ACHK0Z_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU4501568.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7983..8114)
                     /gene="yqgB"
                     /locus_tag="ACHK0Z_RS07630"
                     /old_locus_tag="pgaptmp_001526"
     CDS             complement(7983..8114)
                     /gene="yqgB"
                     /locus_tag="ACHK0Z_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU4501569.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8208..8351)
                     /gene="yqgG"
                     /locus_tag="ACHK0Z_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             complement(8208..8351)
                     /gene="yqgG"
                     /locus_tag="ACHK0Z_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU4501570.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8462..8713)
                     /gene="yqgD"
                     /locus_tag="ACHK0Z_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             complement(8462..8713)
                     /gene="yqgD"
                     /locus_tag="ACHK0Z_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU4501571.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8769..9923
                     /gene="metK"
                     /locus_tag="ACHK0Z_RS07645"
                     /old_locus_tag="pgaptmp_001529"
     CDS             8769..9923
                     /gene="metK"
                     /locus_tag="ACHK0Z_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU4501572.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10359..11753
                     /gene="galP"
                     /locus_tag="ACHK0Z_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             10359..11753
                     /gene="galP"
                     /locus_tag="ACHK0Z_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU4501573.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11830..12327
                     /gene="yggI"
                     /locus_tag="ACHK0Z_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             11830..12327
                     /gene="yggI"
                     /locus_tag="ACHK0Z_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU4501574.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12422..13129
                     /gene="endA"
                     /locus_tag="ACHK0Z_RS07660"
                     /old_locus_tag="pgaptmp_001532"
     CDS             12422..13129
                     /gene="endA"
                     /locus_tag="ACHK0Z_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU4501575.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13209..13940
                     /gene="rsmE"
                     /locus_tag="ACHK0Z_RS07665"
                     /old_locus_tag="pgaptmp_001533"
     CDS             13209..13940
                     /gene="rsmE"
                     /locus_tag="ACHK0Z_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU4501576.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13953..14903
                     /gene="gshB"
                     /locus_tag="ACHK0Z_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             13953..14903
                     /gene="gshB"
                     /locus_tag="ACHK0Z_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU4501577.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15012..15575
                     /gene="yqgE"
                     /locus_tag="ACHK0Z_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             15012..15575
                     /gene="yqgE"
                     /locus_tag="ACHK0Z_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU4501578.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15575..15991
                     /gene="ruvX"
                     /locus_tag="ACHK0Z_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             15575..15991
                     /gene="ruvX"
                     /locus_tag="ACHK0Z_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU4501579.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16180..17160)
                     /gene="yggR"
                     /locus_tag="ACHK0Z_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             complement(16180..17160)
                     /gene="yggR"
                     /locus_tag="ACHK0Z_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU4501580.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            17178..17882
                     /gene="yggS"
                     /locus_tag="ACHK0Z_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             17178..17882
                     /gene="yggS"
                     /locus_tag="ACHK0Z_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU4501581.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17900..18466
                     /gene="yggT"
                     /locus_tag="ACHK0Z_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             17900..18466
                     /gene="yggT"
                     /locus_tag="ACHK0Z_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU4501582.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18463..18753
                     /gene="yggU"
                     /locus_tag="ACHK0Z_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             18463..18753
                     /gene="yggU"
                     /locus_tag="ACHK0Z_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU4501583.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18761..19354
                     /gene="rdgB"
                     /locus_tag="ACHK0Z_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             18761..19354
                     /gene="rdgB"
                     /locus_tag="ACHK0Z_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGU4501584.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19347..20483
                     /gene="hemW"
                     /locus_tag="ACHK0Z_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     CDS             19347..20483
                     /gene="hemW"
                     /locus_tag="ACHK0Z_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU4501585.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20795..21781
                     /locus_tag="ACHK0Z_RS07715"
                     /old_locus_tag="pgaptmp_001543"
     CDS             20795..21781
                     /locus_tag="ACHK0Z_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGU4501586.1"
                     /translation="MKMRSFTRSLVCASLLTLVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETATKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21826..22329
                     /locus_tag="ACHK0Z_RS07720"
                     /old_locus_tag="pgaptmp_001544"
     CDS             21826..22329
                     /locus_tag="ACHK0Z_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4501587.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            22329..23630
                     /locus_tag="ACHK0Z_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             22329..23630
                     /locus_tag="ACHK0Z_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGU4501588.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(23686..24693)
                     /gene="yggM"
                     /locus_tag="ACHK0Z_RS07730"
                     /old_locus_tag="pgaptmp_001546"
     CDS             complement(23686..24693)
                     /gene="yggM"
                     /locus_tag="ACHK0Z_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU4501589.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(24810..25856)
                     /gene="ansB"
                     /locus_tag="ACHK0Z_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             complement(24810..25856)
                     /gene="ansB"
                     /locus_tag="ACHK0Z_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU4501590.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDLATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAQALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(26032..26751)
                     /gene="yggN"
                     /locus_tag="ACHK0Z_RS07740"
                     /old_locus_tag="pgaptmp_001548"
     CDS             complement(26032..26751)
                     /gene="yggN"
                     /locus_tag="ACHK0Z_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU4501591.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(26935..27261)
                     /gene="yggL"
                     /locus_tag="ACHK0Z_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             complement(26935..27261)
                     /gene="yggL"
                     /locus_tag="ACHK0Z_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU4501592.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(27261..27980)
                     /gene="trmB"
                     /locus_tag="ACHK0Z_RS07750"
                     /old_locus_tag="pgaptmp_001550"
     CDS             complement(27261..27980)
                     /gene="trmB"
                     /locus_tag="ACHK0Z_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU4501593.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            28141..29193
                     /gene="mutY"
                     /locus_tag="ACHK0Z_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             28141..29193
                     /gene="mutY"
                     /locus_tag="ACHK0Z_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU4501594.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEMMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            29221..29496
                     /gene="yggX"
                     /locus_tag="ACHK0Z_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     CDS             29221..29496
                     /gene="yggX"
                     /locus_tag="ACHK0Z_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU4501595.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            29561..30640
                     /gene="mltC"
                     /locus_tag="ACHK0Z_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     CDS             29561..30640
                     /gene="mltC"
                     /locus_tag="ACHK0Z_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU4501596.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            30842..32098
                     /gene="nupG"
                     /locus_tag="ACHK0Z_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     CDS             30842..32098
                     /gene="nupG"
                     /locus_tag="ACHK0Z_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU4501597.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(32147..34282)
                     /gene="speC"
                     /locus_tag="ACHK0Z_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     CDS             complement(32147..34282)
                     /gene="speC"
                     /locus_tag="ACHK0Z_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4501598.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCQLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(34444..34527)
                     /gene="yqgH"
                     /locus_tag="ACHK0Z_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             complement(34444..34527)
                     /gene="yqgH"
                     /locus_tag="ACHK0Z_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGU4501599.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            34680..35387
                     /gene="yqgA"
                     /locus_tag="ACHK0Z_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             34680..35387
                     /gene="yqgA"
                     /locus_tag="ACHK0Z_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU4501600.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            35493..35568
                     /locus_tag="ACHK0Z_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     tRNA            35493..35568
                     /locus_tag="ACHK0Z_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35526..35528,aa:Phe,seq:gaa)
BASE COUNT         8694 a         9270 c         8862 g         8957 t
ORIGIN      
        1 ttgcatataa atatttgtga tctacaacac gctttatcta ttgcttgtcc gcaaacggac
       61 atatcaaggt aataaaaaag gtcgccgaag cgaccttttt acccgaaatg ctaattacag
      121 cagttctttt gctttcgcaa cgacgttgtc gacggtgaag ccgaactctt cgaacagcaa
      181 ctctgccgga gctgactcac cgaaggtggt cataccgatg atagcgccat tcaggccaac
      241 atacttgtac cagtagtcag caatacccgc ttctaccgca acgcgtgcag taaccgcttt
      301 cggcagtacg gattcacggt aagcagcatc ctgcttgtca aatgcgtcgg tagacggcat
      361 ggacaccacg cgcgctttca cgccttcggc agtcagtttt tcgtaggcag caacagccag
      421 ttcaacttct gaaccggtag cgatgaaaat cagttccggc tgaccggcgc agtctttcag
      481 cacataacca ccgcgcgcga tgtttgccag ttgctcttca gttcgttcct gctgcgccag
      541 gttctgacgg gagaggatca gtgcggtcgg gccgtcctga cgctcaacac cgtatttcca
      601 cgcgaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg gggttacgcg
      661 cagagaagcg acctgctcaa ccggctggtg agtcgggccg tcttcgccca gaccgatgga
      721 gtcgtgggtg taaaccatca cctgacgctg tttcatcagc gcagccatac gtacggcgtt
      781 acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaaac caccgtgcag
      841 ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt ggatgtagtt
      901 acccgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt tagacggtgc
      961 caggtcagcg gagccgccga ggaattccgg caacagcggg ccgaacgctt caatagcatt
     1021 ctgagacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag cgataaactc
     1081 tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc atacggcggg taaattcagc
     1141 cgcttcctgc ggataagctt tcgcgtaagc cgcaaacttc tcattccatg cagattcttt
     1201 cgcctggcct gcttctttcg catcccactg cgcatagatt tcagacggga tttcgaacgg
     1261 tgcgtatttc cagcccagct gttcgcgggt cagagcgatt tctgcatcac ccagcggcgc
     1321 accgtgggag tcgtgagtac cggctttgtt cggggaaccg aaaccgatga tggttttgca
     1381 catcagcagg gacggtttgt cagtcactgc gcgcgcttct tctactgcgc gtttgatgga
     1441 tgctgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat
     1501 tgcggtgtcg tcggtgaacc agccttcaac gtgaccatca atagagatac cgttgtcatc
     1561 gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa cttcgtggga
     1621 gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt cgacaatgtc
     1681 gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca taccgactgc
     1741 gttggcaata ccctgaccca gcggaccggt ggtggtttcc acaccagcgg tataacctac
     1801 ttccgggtgg cccggagttt tagagtgcag ctgacggaag tttttcagtt cttccatcgg
     1861 cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt ggccgttgga
     1921 cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt tcaggaaatc
     1981 acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt gaccggattt
     2041 ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct ctttacgtga
     2101 ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt gacgacagcg
     2161 cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc gtcatgtaca
     2221 tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt gcgcaatcta
     2281 ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcggttca cctgcaaacg
     2341 gcgcattttt agaataatcc tgaccttgtg cggaagaaaa aacatgaaaa ttcgcgcctt
     2401 attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg actccaacgg
     2461 actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg cgcaggtgaa
     2521 aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg cgccagccaa
     2581 tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca atatcaacgg
     2641 tcagccagtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg caatggctaa
     2701 cggctgtatc cgcgtctata gcgggctgat ggatatgatg acggataacg aagtcgaagc
     2761 ggtgatcggt cacgagatgg ggcacgtggc gttaggccat gtgaagaaag ggatgcaggt
     2821 ggcgcttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga ttgtcggcag
     2881 tttatctcaa tcacaacttg gtgatctggg cgagaaatta gtcaattcgc aattctccca
     2941 gcgccaggaa gcagaagccg atgattattc ttacgatctt ctacgccaac gcggcatcag
     3001 cccggcaggt cttgccacca gctttgaaaa actggcgaaa ctggaagaag gccgccaaag
     3061 ctcaatgttt gacgaccatc cggcatccgc cgaacgcgcc cagcatattc gcgatcgcat
     3121 gagcgcggat gggataaagt gatttcatat ttctctgaca ggaatgtcgg agaaattttc
     3181 attcagagcc ggccagagtc attaaacaag ctcaatcatt tggggcatca attcgcttcg
     3241 tagattgctt tatctggtgt atccggttgc acctctacta catcggcagt gcttacgcca
     3301 caagcccagg caaaagccgt ctctgttcct tcaggtaatg gagtatccca gggtaaagta
     3361 cgccacggac tcccctgctc ttttaataaa gatgtaatgg ctttcttaaa aagaatatgg
     3421 gtaatcaggt gccgtttatc cgttgctttc cagtaccaac gggccagcaa aaaatgcccg
     3481 gccaattcgc gccagcttct gtaatgttgc tgtacgcgtc tggctgccag catcgaaaaa
     3541 tcgaccattt cacgctgggt aatgtatccc acttgcgccc cgctattaca tagcatgaca
     3601 aaacgcacca tatcccaggc acaaaaatct atatcgcata ttttacgtcg attttttctg
     3661 accatctgta gctgccacaa acgctctttc gcttcgctac ttgatttcgc gttctgagct
     3721 aattgctgag cgaacgcgcg atactcagca tccggcattt cgctatcgtt tttgatctgt
     3781 tctgcataaa ctgaacggtg ccctgctaca aacaaagcta agagttgctc aagtaacgaa
     3841 tcccgatcaa agacgcccca catgtcagca agtgactcaa cttcactttt gcaatcattc
     3901 tctgcttcca cggaatcatc attaacgact cgtcgcgccc agtgattgtt acccgcaatg
     3961 gcataaggcg cgccgactgc cattgcccaa cgttcggcag caggaatttt ttgaattgag
     4021 ggatctttac aggatgcttt gaaaaggcga tgtccggcga acaccaggcc ataaccaatc
     4081 cacaaaacag ccaccaaggc gagaataagt attatcgcta ccagataaag ggcatatttg
     4141 cctattgtca gtaagacatc cagccaggaa gagtctggct ccgtaactgt ttgctccacc
     4201 gatgtcaaag gggaattact gacccgctct ggcgcggtcc tcgtcaacag agggaaatcc
     4261 ctatgttgct tgaatgagcg ttttttaagc aaatctattt ttttctgcac ctcatcagta
     4321 acccgaccgt ttttttcaaa ctcatgtaac gatgcaaaat caatcgccgc aatcttccat
     4381 ttaccatccc cttcacggat taattccatt ggaatatcaa tataagattg gtgaccaaaa
     4441 aaagcaccaa tctgcgcctt gtcgccttcg acgaaggtcg ctaaaggatc gctataactg
     4501 gggaaacgtc gtttacacgt tttgggtgac tcttgccagt atttcagtac aggctccatt
     4561 aaagcaggcg taaatacttg ctgcatttcc gcaaggcgat cgtgcgaatc tgtgctggtg
     4621 acaaactcag ggccacataa tgcgcgataa ctctttttga cgacagagga aatagcttct
     4681 ttgtcttgtt gcagctgtgc ggcaattttc tgtttttctg ctctatcgca gccagttgat
     4741 aaaagaaata acaaacaaag aagaggcttc cagggaagag tgcgttccat ctatttattc
     4801 cattaaaaac acggtaataa caatccatta aaaaaacagc agtcagggta aagatgactt
     4861 aattaacagg ataggtttta aatgataagt cccttgtgcc agacaaggga ctgtgcaaaa
     4921 aattcgacca ggcaggttac ttactcgccc tttttcgccg cctgaatata cagcatttcc
     4981 agcgccagcg ttgccgctgc cagagcagtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac gatgttgaga tctttcaggc cgcgtaccag tttaatggcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggttc ccggcgcgaa agcaggatcc
     5161 aggcagtcga tatcaaaggt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaaaccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatggtg ccgtggtcaa attcacaacc gttcgcgtag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaaag agagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg tgcctgcagt ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcaggcgct cacgcatgtc gaaattccac
     5641 gggaagcgat tgtgttccca ggcaagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgtcccc ccgcacgacc agaagtggcc atatcgaacg gcacaccagt aatcacccag
     5761 tcagcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcgttg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa tacggactta
     6001 ctcatcttcc agataagtat aaccgtacaa acctgcctcg aactcttcaa ggaactgttg
     6061 ttgcagttca gcatcaagat cggttttctt cacttgatcg cggaactgag ttaacagcgt
     6121 tttcggatcg agctgcacat attgcagcat atccgcaacg gtatcgcctt cgtcagacag
     6181 ttccacttct acactaccgt cagggaagac gaacacgtca accgcttcgg tatcaccgaa
     6241 caggttgtgc atgttgccga ggatctcctg atatgcgccg accataaaga aaccgagcat
     6301 cggcggattc tctggatcgt actccggcat tggcattgtc gtggcaatac catcaccatc
     6361 aatatagtgg tcgatagcac cgtcagagtc acaggtaata tccagcagca cagcgcgacg
     6421 ttccggcact tgatccagcc cttccagcgg cagaaccggg aacaactggt cgatccccca
     6481 tgcgtccggc attgactgga acagcgagaa attgacgtac attttgtccg ccatacgttc
     6541 ctgcagttcg tcgattatcg gacgatgcgc acggttttgc ggatccagtt gcttctgcac
     6601 ttcatggcac atgctcagat agagttgttc tgcccatgca cgttcttgca ggctaaaggt
     6661 gccggaagag tagccgatat gaatgtcgtg cagatccatc tgactgtcgt gtaaccattc
     6721 acgcagagaa cggcgagttc ccggttcgtg catctcctgc caggtttccc acatgctttg
     6781 cagcgcgcgc ggcgcatctt ctgcaggcgc ggtcggcacc gtgtattcgt tacgttccac
     6841 gccgatgata ttagacacca gcacggtgtg atgcgcagtc accgcacgac ccgattcggt
     6901 gattaccgtc ggatgcggca gaccgttttc ttcacacgca tcaccaatcg cccagatgat
     6961 gttgttggca tattcattga ggccgtagtt tacggagcag tcagactgcg aacgggtgcc
     7021 ttcatagtcc acgcccagac cgccgccgac gtcgaagcac tgaatattga cgcccagctt
     7081 gtgcagttcc acatagaaac gcgccgattc acgaacgcct gtggcgatat cgcgaatatt
     7141 cgccatctgc gaaccgaggt ggaagtgcag tagttgcagg ctgtcgagac gcccggcttc
     7201 acgcagggtt tcaaccagtt gcagtacctg agtcgcagcc aggccgaact tcgatttttc
     7261 cccgccggag gactgccatt tacccgagcc ctgcgaagcc agacgtgcac gcacgcccag
     7321 acgaggaacg acattcagac gttctgcttc atccagcaca atggcgattt ctgacatctt
     7381 ctcaatgacc agatagacct tgtgccccat cttctcgcca attaatgcca ggcggatata
     7441 ttcgcggtct ttataaccgt tgcagacgat gacgctacgg gtcatgccag catgtgccag
     7501 cactgccatc aattcggctt tggaaccggc ttccagaccc agcggttcgc ccgaatgaat
     7561 tagggactca atcacgcgac ggtgctggtt aactttgatc ggataaacaa ggaagtaatc
     7621 gccgttatag ccgtaggatt ccctcgcacg tttgaacgcg gcgttaatgg aacgcaaacg
     7681 gtgctgcagg atctgtggga aacagaacag tgcaggcaga cgctgaccct gtgcttcacg
     7741 agttttcact aactgcgcga gatcgacgcg agcttccggg acgtccgggt ccgggcacac
     7801 gctgatgtgg cccagctcgt taacgtcata gtagttattg ccccaccagg caatattgta
     7861 agtacgcagc atcttgctgg cttcctggga gctcattgca acctcctgca tggagcgtag
     7921 tacaccgtgt tcgcccgctg acgaaggcaa acccatagac atgtcgtcag acatagcgaa
     7981 cctcaaatta ttttattaag tgtaaaacag ttaacgacta tcgcagcctg gaactgcgat
     8041 aacaacccat aagcacatgg attttccagc agtgaatgct gacgctccaa ctgcgcaacc
     8101 ggtttctttt tcataacatt attaagcaca taaccgaacg taagtgtgac agttcggcga
     8161 aaccacgaga aaactcttgt ttttacaaga gcgcccttgt tcagtcctca gtaactgtaa
     8221 ccagctcttg aatcctgaga agcgccgaga tgggtataac atcggcaggt atgcaaagca
     8281 gagatgcaga gtgcggggaa cgaatcttca ccagaacggt gagacaggtt aagcagcaga
     8341 caacggttca ttatttcgta tcacctccac ggccgcctgt taagacgaac ccacaagcca
     8401 aaactctgat ttcaacccgg ctggaagtgg caacacgaaa gaaacgtcgt gtgcttttta
     8461 tttaagccgc gcgccgcgtt ttatacccca caatggcaga aaattgcaaa agataaatac
     8521 gcagaatgcc ggcattgtca ggaaaaattt ccagccacgt ttttaacaga atgagacacg
     8581 attcaaaaaa aagtggaaat cgggtgaaga attgacctaa aatagccatc cagatgttaa
     8641 tccatccata ccgattaaca ctcagactgc cagtgttttt aacttgcaga gtcgtggtag
     8701 gatccgctac cacagaaaat ccacacaaca gtttgagcta accaaattct ctttaggtga
     8761 tattaaatat ggcaaaacac ctttttacgt ccgagtccgt ctctgaaggg catcctgaca
     8821 aaattgctga ccaaatctct gatgccgttt tagacgcgat cctcgaacag gatccgaaag
     8881 cacgcgttgc ttgcgaaacc tacgtaaaaa ccggcatggt tttagttggc ggcgaaatca
     8941 ccaccagcgc ctgggtagac atcgaagaga tcacccgtaa caccgttcgc gaaattggct
     9001 atgtgcattc cgacatgggc tttgacgcta actcctgtgc ggttctgagc gctatcggca
     9061 aacagtctcc tgacatcaac cagggcgttg accgtgccga tccgctggaa cagggcgcgg
     9121 gtgaccaggg tctgatgttt ggctacgcaa ctaatgaaac cgacgtgctg atgccagcac
     9181 ctatcaccta tgcacaccgt ctggtacagc gtcaggctga agtgcgtaaa aacggcactc
     9241 tgccgtggct gcgcccggac gcgaaaagcc aggtgacttt ccagtatgac gacggcaaaa
     9301 tcgttggtat tgatgctgtc gtgctttcca ctcagcactc tgaagagatc gaccagaaat
     9361 cgctgcaaga agcggtaatg gaagagatca tcaagccaat tctgcccgct gaatggctga
     9421 cttctgccac caaattcttc atcaacccga ccggtcgttt tgttatcggt ggcccgatgg
     9481 gtgactgcgg tctgactggt cgtaaaatta tcgttgatac ctacggcggc atggcacgtc
     9541 acggtggcgg agcattctct ggtaaagatc catcaaaagt ggaccgttcc gcagcctacg
     9601 cagcacgtta tgtcgcgaaa aacatcgttg ctgctggcct ggccgatcgt tgtgaaattc
     9661 aggtttccta cgcaatcggc gtggctgaac cgacctccat catggtagaa actttcggta
     9721 ctgagaaagt gccttctgaa caactgactc tgctggtacg tgagttcttc gacctgcgcc
     9781 catacggtct gattcagatg ctggatctgc tgcacccgat ctacaaagaa accgcagcat
     9841 acggtcactt tggtcgtgaa catttcccgt gggaaaaaac cgacaaagcg cagctgctgc
     9901 gcgatgctgc cggtctgaag taatctttct tcacctgctt tcaaaggcca gcctcgcgct
     9961 ggcctttttc ttttacctca ccgcggtgga taggcattca cgccgcatcc ggcaaaaagc
    10021 tgcccgcaca ataacatcat tcttcctcaa cacgttttat cgcaaattat catcactact
    10081 gaaaccgatt acaccaataa caccagccaa atatttgtaa tattttcata ttattattca
    10141 gttttcacag ttgttacatt tcttttcagt aaagtcttaa ttgcagataa cagcgtttaa
    10201 tctatgatga tataactcaa ttattttcat gcacttaaac cataactaag ataaatgtta
    10261 gtgtaagcga ttacactgat gtgatttgct tcacatcttt ttacgtcgta ctcacctatc
    10321 ttaattcaca ataaaaaata accatattgg agggcatcat gcctgacgct aaaaaacagg
    10381 ggcggtcaaa caaggcaatg acgtttttcg tctgcttcct tgccgctctg gcgggattac
    10441 tctttggcct ggatatcggt gtaattgctg gcgcactgcc gtttattgca gatgaattcc
    10501 agattacttc gcacacgcaa gaatgggtcg taagctccat gatgttcggt gcggcagtcg
    10561 gtgcggtggg cagcggctgg ctctccttta aactcgggcg caaaaagagc ctgatgatcg
    10621 gcgcaatttt gtttgttgcc ggttcgctgt tctctgcggc tgcgccaaac gttgaagtac
    10681 tgattctttc ccgcgttcta ctggggctgg cggtgggtgt ggcctcttat accgcaccgc
    10741 tgtacctctc tgaaattgcg ccggaaaaaa ttcgtggcag tatgatctcg atgtatcagt
    10801 tgatgatcac tatcgggatc ctcggtgctt atctttctga taccgccttc agctacaccg
    10861 gtgcatggcg ctggatgctg ggtgtgatta tcatcccggc aattttgctg ctgattggtg
    10921 tcttcttcct gccagacagc ccacgttggt ttgccgccaa acgccgtttt gttgatgccg
    10981 aacgcgtgct gctacgcctg cgtgacacca gcgcggaagc gaaacgcgaa ctggatgaaa
    11041 tccgtgaaag tttgcaggtt aaacagagtg gctgggcgct gtttaaagag aacagcaact
    11101 tccgccgcgc ggtgttcctt ggcgtactgt tgcaggtaat gcagcaattc accgggatga
    11161 acgtcatcat gtattacgcg ccgaaaatct tcgaactggc gggttatacc aacactaccg
    11221 agcaaatgtg ggggaccgtg attgtcggcc tgaccaacgt acttgccacc tttatcgcaa
    11281 tcggccttgt tgaccgctgg ggacgtaaac caacgctaac gctgggcttc ctggtgatgg
    11341 ctgctggcat gggcgtactc ggtacaatga tgcatatcgg tattcactct ccgtcggcgc
    11401 agtatttcgc catcgccatg ctgctgatgt ttattgtcgg ttttgccatg agtgccggtc
    11461 cgctgatttg ggtactgtgc tccgaaattc agccgctgaa aggccgcgat tttggcatca
    11521 cctgctccac tgccaccaac tggattgcca acatgatcgt tggcgcaacg ttcctgacca
    11581 tgctcaacac gctgggtaac gccaacacct tctgggtgta tgcggctctg aacgtactgt
    11641 ttatcctgct gacattgtgg ctggtaccgg aaaccaaaca cgtttcgctg gaacatattg
    11701 aacgtaatct gatgaaaggt cgtaaactgc gcgaaatcgg cgctcacgat taatctcctc
    11761 aggcttcctc ccgtcgcgga ggaagccacc tcttgcagtc atcttttctt tgctctatcc
    11821 tctgccgcta tgaaaacatc ccgtctccct atcgccatcc aacaggccgt tatgcgtcgc
    11881 ctgcgggaaa aactcgccca ggccaacctg aagctagggc gtaactaccc ggagccaaaa
    11941 ctctcttaca cccagcgcgg aacctccgcc ggaacggcct ggctggaaag ctatgaaatt
    12001 cgcctcaatc ccgttttgct gttggaaaac agtgaagctt ttattgaaga agtggtaccg
    12061 cacgaactgg cacatttgct ggtatggaaa catttcggcc gcgtagcgcc acatggcaaa
    12121 gagtggaagt ggatgatgga aagcgtgctg ggtgttcccg cccgtcgtac gcatcagttc
    12181 gaactgcaat ccgtgcgtcg caacaccttc ccctaccgct gcaagtgcca ggagcatcag
    12241 cttaccgtac gccgccataa tcgcgtagtt cgtggcgagg ccgtctatcg ctgtgttcac
    12301 tgcggtgaac agctggttgc gaaataacca tctgaactat caggaacttt cctgatctgg
    12361 ctgattgcat accaaaacaa ctttcgctac gttgctggct cgttttaaca cggagtaagt
    12421 gatgtaccgt tatttgtcta ttgctgcggt ggtactgagc gcagcatttt ccggcccggc
    12481 tttggccgaa ggtatcaata gtttttctca ggcgaaagcc gcggcggtaa aagtccacgc
    12541 tgacgcgccc ggtacgtttt attgcggatg taaaattaac tggcagggca aaaaaggcgt
    12601 tgttgatctg caatcgtgcg gctatcaggt gcgcaaaaat gaaaaccgcg ccagccgcgt
    12661 agagtgggaa cacgtcgttc ccgcctggca gttcggtcac cagcgccagt gctggcagga
    12721 cggtggacgt aaaaactgcg ctaaagatcc ggtctatcgc aagatggaaa gcgatatgca
    12781 taacctgcaa ccgtcagtcg gcgaggtgaa tggcgatcgc ggcaacttta tgtacagcca
    12841 gtggaatggc ggcgaaggcc agtacggtca atgcgccatg aaggtcgatt tcaaagaaaa
    12901 agctgccgaa ccaccagcgc gtgcacgcgg tgccattgcg cgcacctact tctatatgcg
    12961 cgaccaatac aacctgacac tctctcgcca gcaaacgcag ctgttcaacg catggaacaa
    13021 gatgtatccg gttaccgact gggagtgcga gcgcgatgaa cgcatcgcga aggtgcaggg
    13081 caatcataac ccgtatgtgc aacgcgcttg ccaggcgcga aagagctaac ctacactagc
    13141 gggattcttt ttgttaaccc ctaccccacg cgtacaaccg cgtggggaga cgacgcggat
    13201 ttttaactat gcgtatcccc cgcatttatc atcctgaacc actgaccagc cattctcaca
    13261 tcgcgctttg cgaagatgcc gccaaccata tcgggcgcgt actgcgcatg gggccggggc
    13321 aggcgttgca attgtttgac ggtagcaacc aggtctttga cgccgaaatt accagcgcca
    13381 gcaaaaaaag cgtggaagtg aaggtgctgg aaggcaagat cgacgatcgc gaatctccgc
    13441 tgcatattca cctcggtcag gtgatgtcgc gtggtgaaaa aatggaattt acgatccaga
    13501 aatcgatcga actcggtgta agcctcatta cgccactttt ttctgagcgc tgcggcgtta
    13561 aactggatag tgaacgtctg aacaagaagc ttcagcagtg gcagaagatt gcaattgctg
    13621 cctgtgagca gtgtggtcgt aaccgggtgc cggaaatccg tccagcgatg gatctggaag
    13681 cctggtgtgc agagcaggat gaaggactga aactgaatct tcacccgcgc gccagtaaca
    13741 gcatcaatac gttgccttta ccggttgaac gcgtccgcct gctgattggc ccggaaggcg
    13801 gtttatcggc agatgaaatt gccatgactg cccgctatca atttactgat atcctgttgg
    13861 gacctcgcgt tttgcgtaca gagacaactg cgctcaccgc cattaccgcg ctacaagtac
    13921 gatttggcga tttgggctaa cggagaagaa taatgatcaa gctcggcatc gtgatggacc
    13981 ccatcgcaaa catcaacatc aagaaagatt ccagtttcgc tatgttgctg gaagcacagc
    14041 gtcgtggtta cgaacttcac tatatggaga tgggcgatct gtatctgatc aatggcgaag
    14101 cccgcgccca tacccgcacg ctgaacgtga agcagaacta cgaagagtgg ttttcattcg
    14161 tcggtgaaca ggatctgccg ctggccgatc tcgatgtgat cctgatgcgt aaagacccgc
    14221 cgtttgatac cgagtttatc tacgcgacct atattctgga acgtgccgaa gagaaaggga
    14281 cgctgatcgt taacaagccg cagagcctgc gcgactgtaa cgagaaactg tttaccgcct
    14341 ggttctctga cttaacgcca gaaacgctgg ttacgcgcaa taaagcgcag ctaaaagcgt
    14401 tctgggagaa acacagcgac atcattctta agccgctgga cggtatgggc ggcgcgtcga
    14461 ttttccgcgt gaaagaaggc gatccaaacc tcggcgtgat tgccgaaacc ctgactgagc
    14521 atggcactcg ctactgcatg gcgcaaaatt acctgccagc cattaaagat ggcgacaaac
    14581 gcgtgctggt ggtggatggc gagccggtac cgtactgcct ggcgcgtatt ccgcaggggg
    14641 gcgaaacccg tggcaatctg gctgccggtg gtcgcggtga acctcgtccg ctgacggaaa
    14701 gtgactggaa aatcgcccgt cagatcgggc cgacgctgaa agaaaaaggg ctgatttttg
    14761 ttggtctgga tatcatcggc gaccgtctga ctgaaattaa cgtcaccagc ccaacctgta
    14821 ttcgtgagat tgaagcagag tttccggtgt cgatcaccgg aatgttaatg gatgccatcg
    14881 aagcacgttt acagcagcag taacccacct tagcgagaag gatctcgttg agactctgag
    14941 tgacagcgcc cttctttcca cgcatactgg gcgctgttgc ttttttgaac caggaaacag
    15001 aacctctgac aatgaattta cagcatcact ttcttattgc catgcctgct ctccaggatc
    15061 cgattttccg tcgttccgtg gtctacattt gcgaacataa taccaatggt gcaatgggga
    15121 tcatcgtcaa caagccgctg gaaaatctca aaattgaagg gattctggaa aagctgaaga
    15181 tcacgccgga gccgcgtgat gaatcaatcc gtctggataa accggttatg ctcggcggtc
    15241 cgctggctga agatcgcggg tttattttgc atactccgcc ctccaatttt gcttccagca
    15301 ttcgcatttc agacaacaca gtgatgacca cttcccgcga tgtgctggaa acgctcggca
    15361 ccgataaaca accgtctgac gtattggtgg ctctgggtta tgcctcctgg gaaaaaggtc
    15421 aactggaaca agaaattctc gataacgcgt ggctaacggc cccggcggat ctgaatattc
    15481 tgttcaaaac accgattgcc gaccgctggc gcgaggcggc aaaactgatt ggtgtggata
    15541 ttctcaccat gcctggtgtg gcagggcacg cctgatgagt ggaaccttac tcgccttcga
    15601 cttcggcacc aaaagcattg gcgtagcggt cggccaacgc attaccggca ctgctcgccc
    15661 tttgcctgca attaaagcac aggacggtac gccggactgg aacctcatcg aacgtttact
    15721 gaaagagtgg cagccggacg aaatcatcgt cggtttgccg ctgaatatgg acggcaccga
    15781 gcaaccattg actgccagag cgcgtaaatt tgccaaccgt attcatggtc gtttcggtgt
    15841 tgaagtaaag ctccatgacg aacgtcttag caccgtggaa gcccgttccg gtctgtttga
    15901 acagggcggc tatcgggcgc tcaacaaagg caaagttgac tctgcctctg cggttattat
    15961 tctcgaaagc tatttcgagc agggatatta aggcgattta aacgcctgct ggagtgtata
    16021 atatcatcgc gccttacgcc ttattgccgg atgcggcgtg aacgccttat ccggcctaca
    16081 tgtggcagcg attgtaggtc tgataagacg cgttagcgtc gcatcagaca tttattgcct
    16141 ttataggccc tataaacttg cgcatcgggc atggcaacgt cacaaacgcc cttcccccac
    16201 ccgctgctga taactctgct gaaacgttat catccccacc tgctgcccgg tttgaataac
    16261 atgcggtaac tggtgggttt tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat
    16321 caataattca aacagcgcca cgcgtccttc ctgtttatcc acttccagct tttgtgacaa
    16381 cacggcccgt aaactacctg ccagttgatt acgtacggga tctttttcct gcgccggaaa
    16441 tgaatccacc agtcgctcaa ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac
    16501 caaatgcccg gtttctgccg ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc
    16561 tccgagcaaa atcacatcag gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt
    16621 catacagtgc aaaccaattt cccgctgctg gatcaaacat cgctggctgg catagatata
    16681 ttcaacagga tcttccagcg tcagaatatg cgcatcggca tgttgattga gatagccaac
    16741 catcgccgcc agcgtggtag atttgccgct ccccgtcgcc cccgtcacca gaatcaggcc
    16801 attctcgctc ttgagtaatt ccggcaatac cgttggtgcg ccaagctgtt cgagctgtgg
    16861 acagtgcgaa ggtaacaacc gtaatgccag agaaatacct tgccgttgcg cgaacgcact
    16921 gccacgcaac cgctggtttt ccgccagcga cacggcaaaa tccagctggc cattttccag
    16981 caatattgcc cgctgatcgt catccagcca ctcccgcagt agctcttcga cgtccggcgc
    17041 atcaaacggc gcagcttcca ttctgccacg aatgcgccat cgtgcgggcc aggcgctgca
    17101 caggtgtaga tccgagacgt tatgctttac actaagggcc acaatttctt ccatattcat
    17161 actaagatcc tcggaaaatg aacgatattg cgcataacct ggcacaggtc cgggacaaaa
    17221 tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg cttgcagtca
    17281 gtaaaacaaa acctgcgagc gccatcgcag aagccattga tgccgggcag cgtcaatttg
    17341 gtgaaaacta cgttcaggaa ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa
    17401 caggattaga atggcatttt attggcccgt tgcagtctaa taaaagccgc ctggtggcag
    17461 agcatttcga ctggtgtcat accatcgacc gtttgcgcat tgctacccgc ctcaacgatc
    17521 agcgcccggc agaacttccc cctcttaacg tactgattca aattaatatt agtgatgaaa
    17581 acagtaagtc cgggattcaa ctggcagaac tggacgagct ggcagcggcg gtcgctgaac
    17641 taccgcgttt acgtctgcgc gggctgatgg caatccctgc gcctgagtca gaatatgtaa
    17701 ggcagtttga agttgcacgc caaatggctg tagcatttgc cggactgaaa acgcgctacc
    17761 cgcatatcga cacgctctct ctgggaatgt cggacgatat ggaagccgcc attgcggcag
    17821 gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat
    17881 aaggaattaa aggaacgcca tgaatacgtt gactttcctg ctttcaacgg tcattgagct
    17941 gtataccatg gtgctgttat tacgcatctg gatgcagtgg gctcattgtg atttttacaa
    18001 ccccttctca cagtttgtag tgaaggtaac acagccaatt atcgggccac tgcgccgcgt
    18061 tattccggca atgggtccca ttgatagcgc ctcgctgctg gttgcgtaca ttctcagttt
    18121 tatcaaagcc atcgtgctgt ttaaagtggt gaccttcctg ccaatcatct ggattgccgg
    18181 tttactgatt ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc tggtgatggc
    18241 gattatgagc tgggtaagcc agggtcgtag tccgattgaa tacgtgctga ttcagctggc
    18301 cgatccgctg ctgcgcccga ttcgccgcct gctaccggca atgggtggga ttgatttctc
    18361 gccgatgatc ctcgttctgc tgctgtatgt catcaatatg ggtgtcgcag aagtattaca
    18421 ggcaaccgga aacatgctgc tgccggggct gtggatggcg ttatgagtgc cgtaacagtt
    18481 aatgatgacg gtctggtttt acggctctat attcagccga aagccagccg tgattctatt
    18541 gtcggtttac atggcgacga agttaaagtc gccattaccg cgccgccggt tgacggccag
    18601 gccaacagcc atctggtgaa gtttctcggt aagcaattcc gggtagccaa aagccaggtg
    18661 gtaattgaaa aaggcgaact tggccgccac aaacaaatta aaatcattaa tccgcaacaa
    18721 atcccgccag aaatcgcggc gttaattaat taggtatcct atgcaaaaag ttgtcctcgc
    18781 aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga
    18841 tatcgtggcc caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat
    18901 cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg accggtttac cggcaattgc
    18961 cgacgactct ggtctggcgg tagatgcgct gggtggcgcg ccgggtattt actccgcgcg
    19021 ttattccggt gaagacgcga ccgatcaaaa gaatctgcaa aaactgttgg aaacactgaa
    19081 agacgtaccg gacgaccaac gtcaggcgcg tttccactgc gtgctggtat atctgcgtca
    19141 cgcggaagat ccgactccgc tggtgtgcca cggtagctgg ccgggcgtga ttactcgtga
    19201 accagcgggc actggtggct ttggttatga tccaatcttc ttcgtacctt ccgaagggaa
    19261 aaccgctgcc gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcatt
    19321 gaaactgctg ttggacgctt tacgtaatgg ttaaattacc gccgctgagt ctctacattc
    19381 acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt caactctcac gcgttgaaag
    19441 gagaagtgcc gcacgacgat tacgttcagc atctgctttg cgatctggac aacgatgtgg
    19501 cttacgctca gggccgtgaa gtaaagacaa tctttattgg cggtggtacg ccgagcctgc
    19561 tttccggccc ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag
    19621 cggatgcaga aattactatg gaagcgaacc ctggtacggt agaagccgat cgctttgtcg
    19681 attatcagcg tgctggtgtg aaccgcatct ctattggtgt gcagagtttt agcgaagaca
    19741 agctgaaacg acttgggcgt attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg
    19801 cgagcgggct ggggctgcgt agctttaacc tcgatttgat gcatggacta ccggatcaat
    19861 cactggaaga ggcgcttggc gatctacgcc aggccattga actgaatccg ccgcatcttt
    19921 cctggtatca actgaccatc gaacctaata cgctgtttgg ttcacgcccg ccggtactgc
    19981 cggacgacga cgcgctgtgg gatatattcg aacaggggca tcagttatta accgcagcgg
    20041 gttatcagca atatgaaact tccgcttacg ccaaacccag ttatcagtgc cagcacaatc
    20101 tcaactactg gcgctttggc gactacatcg gtattggctg cggcgcgcac ggcaaagtga
    20161 ccttcccgga tgggcgcatt ctgcgtacca ctaaaacgcg tcatccgcgt ggttttatgc
    20221 aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc agataagccg tttgagttct
    20281 ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg
    20341 ggctttgcga agatgtgatt cgcccacagt tagacgaggc gattgcccag ggttatctca
    20401 ccgaatgtgc ggattactgg cagataacgg aacatggtaa attattctta aactcacttc
    20461 ttgaattatt cctcgctgag taatcattaa tgcgtctgcg ggcgcattaa tcacttcttc
    20521 cccccctccc ccgttaatta acttcggcaa aatatatttc ttactgaata aatatcgacg
    20581 atatttatta tatatagtga atatttactc gtcatttatt gattataaac atgtacaacc
    20641 tggcgcacaa atttaatatt taatttctga ctttatttca agttgtgata aacatagaca
    20701 agttgtcaga caggttaacc aatttgtcgc acaaagcact gataagcaca attagatatc
    20761 aaccaatttc catccttcaa cagggagttt ttttatgaaa atgaggtcat ttacccgctc
    20821 attagtctgc gcatccttgc tgactctggt tagcactggc gtaaacgccg cagaaaaagt
    20881 gacgttaaag ttagcacaca acctggaaag aagtcatgtt gtacatcaga gttttgaaga
    20941 gctggcgaaa gaggtaaaac agctgtcaaa aggtaatatg gtgatccgta tttatcccag
    21001 cagccagatg ggtaatgcgc gagaaactat ggagttgttg caaaatggcg ctctggatat
    21061 gaccaaaggt tccgccagtg atctggagtc ttttgataat atttatgcta tctataattt
    21121 gccgttctta tttaaagatc aggctcactt caacaaagtt gtttttggtg aagttggtaa
    21181 agaaattatg gactcaacaa aagacaaagg cttttttgct ttatcagctt atgtagcggg
    21241 tacacgcagt ttctatgcga aaaaaccgat tactaagcca gaagatctta aagggctgaa
    21301 aattcgtgtt cagccaagtc cgaccaccat caaaatgatt gaactgatgg gtggttcacc
    21361 cactccaatt tcatttggcg aagtttatac tgcgatgcag caaggtgtag tagatggcgc
    21421 agaaaataac gtgccttcct gggtgcagac tcgacatatt gaaattgcca aagttttttc
    21481 tgaagatgaa cacgcctcaa ttcctgactt tttggttatc tccaccaaaa catggaataa
    21541 attaacccca gaacagcaac aaatcctcga aacagcaacg aagaaatccg aagcttatca
    21601 acaaaaattg tgggaaaaaa ttgacgctga cacacgtgct caggcgaaag ctatgggggg
    21661 tgaaattgta aaagtggata aagcaccatt ccgtgccgca gttcagcctc tgtttgacga
    21721 cttcaaaaaa gatccaaaac aagctgcttt gttagagaag ttcgacaacg ctgctcaata
    21781 atattccggg ccagtctctg gccctgattt tccaggatgc ttacaatgat atttaaccgc
    21841 ctgaaacttg cggtagaccg cgtcattgcc gcgttttctg tcgccgtcat gcttgctctg
    21901 gttgtatgcg tagtctggca agtatttagc cgctacgtac ttaaccagcc aagtacatta
    21961 accgatgagc tggcacgatt tttgatgatc tgggtagggt tattaggtgc tgcatatacc
    22021 gtcggcgcgc agagacatct ctctatcgat ttatttgctc tcgccctgaa taagcgcaag
    22081 caattacttc ttagtatagt cattaacgtt cttattttag gttttgccgg ttcggtcatt
    22141 gtgactggtg gcctgaaatt aattgataaa acgctggcaa catcacaggt atctgccgcg
    22201 atgcaaatac cgatgggata cgtatatatc attctgccat taagtggatt ggtgatgatg
    22261 ttttatgcct tgtgttttat taatcaaagc attcaacaac tgaaacaaca tgcgcaggag
    22321 gccagttaat ggatagttat atcgcgatta cactgtttgg ctgttttttt gtgttggtct
    22381 ttattggtgt gcccatttcg ttctcaatcg gtattgccac tgtagcttca atgcttttga
    22441 tgtttccctg ggatatcgcg gcaatcactg tttcgcagcg actggctaat ggactggata
    22501 actttgccct attggcaatc ccctttttca tctttgccgg aacgctgatg aacagcggcg
    22561 gtattgcgat acgtttgatt aacctcgccc aggtaatggt tggtcgggtt cctggttcac
    22621 ttggtcatgt gaacgtcctg gcgaatatga tgtttggttc catttctggt tcagccgttg
    22681 cggcagctgc tgccgttggt ggcacactga atcccattca gaccaaacaa ggttatgatc
    22741 cggcattttc taccgctgta aacgtctcat cttgtattac tggattactg ataccgccct
    22801 caaacgtgtt aattgtgttt tccctgacag ctggcggcgt ttctgtagca tcgctattta
    22861 tggcgggata tttgccaggg atcttaatgg gtcttgcggt aatgatagtc tgcggcatta
    22921 ttgctaaacg ccgtggctac ccgctttcag aaagagcaac ctttgctcag gcctgtaaag
    22981 cgtttctgga tgccttacca agtttgcttc tggtgttcat tgtgatgggc ggcattctgg
    23041 gcggtatttt taccgcaact gaagcgtcag caattgccgt tgtctacaca tttattctct
    23101 cggtgctgat ttaccgggaa gtaaaatggc gccatttacc caaactgatt ctggagtcgg
    23161 ttgttaccac ttcgattgta ttactgttga tcggtttttc cgtgggcatg tcctgggcaa
    23221 tgactaatgc tgatattcct tatatgatca gcgacgcatt gatggggatt tctgataacc
    23281 cactgatcat attgctgctg attaatatcg tcttattaat tgtcggcatc tttatggaca
    23341 tgacaccggc tgtactaatc tttacaccga tcttcttgcc gattgcacaa gagctgggaa
    23401 tggacccagt acatttcggt attatgatgg tggccaacct ttgtattggg ctgttaacac
    23461 cgccggtggg tagtgcgcta tttgttggtt gttctatctc gggtgtgaag attcaacatt
    23521 taatcaaacc attgctgcca ttttatgctg cattgctagt agcgttgatg atgatcactt
    23581 acattccgca aatatccttg ttcattccac agctactagg gctaatgtaa aaaataattt
    23641 gccggataac tttttatatt atccggcaaa ggcactatat caaacttact taatcacccc
    23701 attgcgccgc agttgttcgg tatctttttt ccagcgttca ttagcttggt tgatatcgtc
    23761 aattttcatc aaaatcgcga cttttttact gtttatttct gaaactttca tcgttaacgg
    23821 atattgccct ttttcgagcg ttacccagtc atcctgcaat ttacggctga gtttttgtga
    23881 ttgcagaaaa gtctgtcgcc gttgttcgtt gatatcgaaa tctcgtttct gctgaatcgg
    23941 cacttcataa cactcttcac tctgtttatg acatgccgca atcgccggtt gataaacctc
    24001 tttgtcatat ttaaccgcga acgcctcgct atcgttctgt ttattaaact catcacgctg
    24061 ttggtgaatt tttttaaagg cttcttcgcg ggtcatttgc ttaccgtttg catcttttcc
    24121 ccaatatgca tccgctgctt tctgcgccgc caccatttgc ttatcaagcg ctttcacttc
    24181 cttttccagc gcaggaatcg agttcttctg ggtattcaac gcagattcaa gctgactaaa
    24241 gcgataattg aaatcttcgc tattcacgat cagatattta ttattcgttt tgatatcgtc
    24301 gaccacccgc ccacgatcgc tggctgccgc ctgaaaagag taaaagttca ccgaccagcc
    24361 agacgcgggc gttcctttac tggttaacat cgccgagaat tttaccggtt tatctttcgt
    24421 ccaggtctgt tcgagcagtt cgtaatctgc caactgaccg acccaggtat agagatcgtc
    24481 actggaagag acatcgcctt ccgctgacca ggtggcctga ttacccttag catcaagatt
    24541 ttttaaggtg atagcatcaa gcttgagaat gccgtgatac tgctttttga attgatcttt
    24601 taagatattt tcagttggcg gttcgccatc tgcccaggca ttaccagtca tcaacataag
    24661 cagtgccgtg ccaacaatcc attgcttttt cacccgaaaa acctcacgcg gaataattta
    24721 ttccggtgat tatcattagg gtaatgaaaa ttggcaatgg acgtaagtct gaagtgaaaa
    24781 agccccggca cgataccggg gcgaggtgat tagtactgat tgaagatctg ctggatctgc
    24841 tgcggatctt tggtttgcgt cagagccagc tgcagcagga cgcgcgcttt ttgcgggttc
    24901 agcgtgccag aggcgacgaa gccgtatttc gcatcatcca cttcagcatc ctgagtggta
    24961 gcacccgtcg gtacgcggga agaacgcact actgcagtgc cgtttttcgc ggcggttgcc
    25021 agggtgtcga acacggattt atacaggtta ccattaccca caccagcgct aacgatgcca
    25081 tcatagcccg catctaccag tgcttgagcc ggaagatcgg atgcgttagc gtagttataa
    25141 acgatgccaa ctttcggcag ctcattcagc ttagagacat cgaatggcgt atcgctggtg
    25201 tgcttacgtg ccggggtacg ttggtagtca atcttaccgt tgtgaatgta tcccagagga
    25261 ccgtagttaa cagacttgaa ggtcgctagg tcggtggtgt tggttttggt gacatcgcga
    25321 ccgtccagta cggtgtcgtt catcaccacc agcacgccac gattagcgga ggctttatca
    25381 gctgcggtca ctaccgcgtt atacaggttg aatggaccgt ctgcgctcat ggacgtggac
    25441 gggcgcattg cgccgaccat caccaccggt ttgtcgcatt tcaccgtcag gtcgaggaag
    25501 taagcggttt cttccatcgt gtcggtaccg tgggtaatga cgaagccgtc ggttttatcg
    25561 cagtcggtgt taattttttt cgccagtgtc agccagacat tatcgttcat gtcctgggag
    25621 ccgatattca ctacctgctc gcctttaacg ttcgcaatgt cttttagttg cggcaccgca
    25681 ttaaccagat tttctacgcc aactttaccc gctgtgtagt tagatttggt tgcggagtca
    25741 ccaccaccgg caatggtccc gccggttgct aaaatggtga tattgggtaa tgccaatgct
    25801 gcaccactaa aacccataac cagtgcggca agtgccgtct ttttgaaaaa ctccatttca
    25861 ttcctccagt tacgtgaacg ctacgcatta tcccttagct ctgtatggga aatttgacgt
    25921 taaacaattt acaacgtgaa tatattttgg agatctacaa agttagaggc aggtaacaaa
    25981 acgaagaatt aaacggcata aaaaagtatt atgccgtctt aaaatagagg attattttaa
    26041 attcccgacc agggctttgc ggctatcttc cagagtcaca acgcggctac aaacatcttt
    26101 gccaaactgc tggaaatctt tttcctgctt tttccactcg gtttggattg aggattgcag
    26161 cccccccagg cttcccagca cattctgtaa tgggttaccg ccgcttttca gcaccgcttt
    26221 cgcgcccatt tcattaatgc tgtcctgtaa aattccaccc attgcctgat tcactaattg
    26281 ctggccttcg gcgcgaacct gatcaatggc tttatagtga aacgtcaggc catcgctgcg
    26341 cgtttcgata atgcggttca tctgctcttt cagctgcgca tcaagtttgg tcagacggct
    26401 gcgcattttg ctgctttcgc ccatctcctg aacgataatt ttatccagcg caatacgggc
    26461 tttttcgacg cggcttttcg cgccttcatc aatccacggc agcgtgctgc gtagttcagc
    26521 ctgataatcc ttcgcctgct cgcgctgggc ggcattcagg gaatattgct taccgttata
    26581 catcacgttg ccgtctggcg tgatcaccag attgccgttt tcgcccttca cctgcacggt
    26641 ttgcgggctg acaatcacat cgtcacgcgg cgtgacgctg cactggtagt cggcgtgagc
    26701 ggtcattgcc gtcactgaaa gtgctgccgc cagcagcatt ttgcgcatca tagtcttccc
    26761 tcaagaaaaa atcaggccag catttgctgg ccccagattg ataacaaagt acgcgttttc
    26821 catgccggat gcggcgtaaa caccttatcc cgcctactcg atcgtgcatc tttccaggcc
    26881 tgataagcgt agcgcatcag gcagttttgc atttgtcatc gcccgtatgc tttcttagtc
    26941 ccaccaaacg tcgaaaagtt cgctggtgcg tacctcatcc agtttgcgct cttccagcca
    27001 cttacgcaca atcgcctgat gttcttcggt gcatttgccg atttcctgca tgcagatcag
    27061 accttcccag gccagataac cgctgccgtc aaaggccagt ttgttcggtt cgataacctc
    27121 gttaataaaa tcatcaacgg ttttatcaat ctgttcttcc gatgtacctt ccgggaatcg
    27181 ccatgccacc gaaaatccta attcctggaa ttcgtcgatg tgcatttttt tacgcagacg
    27241 acggctacgg ttctttgcca ttatttcacc ctctcgaaca ttaagtccca tactccgtga
    27301 ccaagacgat gaccacgttg ttcaaatttc gtcaccggac gtgatgccgg acgcggtacg
    27361 taatcattgc tctctgacag gtttttataa ccgtcaatag aagacatcac ttcaagcata
    27421 tgttccgcat aaggttccca gtcggtcgcc atatggaata cgccccccag ctgcagtttg
    27481 ctttttacca gttcggcaaa cggcacctgt acgatacggc gtttattatg gcgcgctttg
    27541 tgccacgggt cagggaaaaa gagctgcacc atgcgcaatg aattgtcagg aatcatttta
    27601 tgcagcactt caaccgcatc gtgacacatc actcgcaggt tgcttaaacc ttcttcatgc
    27661 gcagaagcca ggcacgcacc aacgcccggt gaatgcactt caatgccgag gaagtcctgc
    27721 tcagggcgat ctttagccat tgccaccagc gacgccccca tgccaaaacc aatctcaagc
    27781 gtcaccggcg cttcacggcc aaaaagcgcg gggaaatcca gcatatcttc gctgaactca
    27841 acgcccatca ccggccagta gttttccagc gcatgttcct ggcctttggt cagtcgcccc
    27901 tggcggcgca caaaactacg gatacggcgc agtgggcggc cgttttcatc aaattccggt
    27961 gaaatgacgt cgtttttcat aaagatttag tcgcttgtga aagtgttctg aaaacgggca
    28021 ttatccaaag ttagttgccg gatgcaagca tgataaggcc gtggctgcgg aaagttccgg
    28081 tttacaccct gccgtcgctg tgctgcaatc ttgcccccaa caacagtgaa ttcggtgacc
    28141 atgcaagcgt cgcaattttc agcccaggtt ctggactggt acgataaata cgggcgaaaa
    28201 acgctgccct ggcaaattga caagacgccc tacaaagtat ggctctcaga aatgatgttg
    28261 caacaaactc aggttgcgac cgttatcccc tattttgaac gctttatggc gcgcttcccg
    28321 acggtgaccg atctcgccaa tgcgccgctc gacgaagttc tccacttgtg gaccgggctt
    28381 ggctattacg cccgcgcgcg caatctgcat aaagcggcac aacaagtggc gaccttacac
    28441 ggcggtaaat tcccggaaac ctttgaagaa gtcgcggcgt taccgggcgt cgggcgttcc
    28501 accgcaggag cgattctctc gctttctctg ggtaagcact ttccgattct cgacggtaac
    28561 gtcaaacgcg tgctggcgcg ctgctatgct gtaagcggct ggcctgggaa aaaagaggtc
    28621 gagaataaat tatggagttt gagcgagcag gtgacgcccg cggttggcgt ggaacggttt
    28681 aatcaggcga tgatggattt gggtgcgatg atttgtacgc gctcgaaacc gaaatgttcg
    28741 ctctgtccgc tacaaaacgg atgtattgcc gccgccaaca atagctgggc gctttatccg
    28801 ggcaaaaaac cgaaacagac gctgccggag cgcaccggct actttttgct attacagcac
    28861 gaagatgaag tattgctggc gcagcgtccg ccgagcggat tgtggggcgg tttatactgt
    28921 ttcccgcagt ttgccgacga agaaagtttg cggcattggc tggcgcaacg gcagattgct
    28981 gccgataacc tgacgcagct gaccgcgttt cgccatacct tcagccattt ccacttagat
    29041 attgtgccta tgtggcttcc cgtgtcgtca ttcaccggct gcatggatga aggcaatgcg
    29101 ctctggtata acttagcgca accgccgtca gttggcctgg cggctcccgt ggagcgtttg
    29161 ttacagcaat tacgcactgg cgcgccggtt tagcgcgtga gtcgataaag aggatgattt
    29221 atgagcagaa cgattttttg tactttcctg caacgtgaag cagaaggtca ggattttcag
    29281 ctgtaccccg gcgagctggg aaaacgcatc tataacgaga tctccaaaga agcctgggcg
    29341 cagtggcagc acaagcaaac catgctgatt aatgaaaaga aactcaacat gatgaatgcc
    29401 gagcaccgca agctgcttga gcaggagatg gtcaacttcc tgttcgaggg taaagaggtg
    29461 catatcgagg gctatacgcc ggaagataaa aaataaaaac agtgccggag cacgcctccg
    29521 gcaacttgca taaaaacaaa cacaacacgc acccggaatg atgaaaaaat atctcgcgct
    29581 ggctttgatt gcgccgttgc tcatctcctg ttcgacgacc aaaaaaggcg atacctataa
    29641 cgaagcctgg gtcaaagata ccaacggttt tgatattctg atggggcaat ttgcccacaa
    29701 tattgagaac atctggggat tcaaagaggt ggtgatcgcc ggtcctaagg actacgtgaa
    29761 atacaccgat caatatcaga cccgcagcca catcaacttc gatgacggta cgattactat
    29821 cgaaactatc gccgggacag aacctgccgc gcatttgcgc cgggcaatta tcaaaacgtt
    29881 gctgatgggt gacgatccga gttcggtcga tctctattcc gacgttgatg atattacgat
    29941 ttcgaaagaa cctttccttt acggtcaggt ggtggacaac accgggcagc cgattcgctg
    30001 ggaaggtcgc gcaagcaact tcgcggatta tctgctgaaa aaccgtctgc aaagccgcag
    30061 taacgggctg cgtatcatct acagcgtcac cattaacatg gtaccgaacc accttgataa
    30121 acgtgcgcac aaatatctcg gcatggtccg ccaggcgtca cggaaatatg gcgttgatga
    30181 gtcgctgatt ctggcaatta tgcagaccga atcttccttt aacccgtatg cggtcagccg
    30241 ttccgatgcg ctgggattaa tgcaggtggt acaacatact gccgggaaag atgtgttccg
    30301 ctcgcagggg aaatccggca cgccgagccg cagtttcttg tttgatcctg ccagcaatat
    30361 tgataccggc accgcgtatc tggcgatgct gaacaatgtt tatctcggcg gaattgataa
    30421 cccaacatcg cggcgttatg ccgtcatcac cgcctataac ggcggcgcag gcagcgtgct
    30481 gcgagtcttt tcgaatgata agattcaggc tgccaatatt attaacacca tgacgccggg
    30541 cgatgtttat cagacgctga cgacccgcca tccctctgcg gaatctcgcc gttatcttta
    30601 taaagtgaat accgcgcaaa aatcctaccg ccgccgataa ttccattaac cgcccctgac
    30661 gatgctcagg ggcaaaaatg ttatccacat cacaatttca ttttgcaaat tgggaatgtt
    30721 tgcaattatt tgccacaggt aacaaaaaac cagtccgcga agttgataga atcccatcat
    30781 ctcgcacggt caaatgtgct ttttcaaaca ctcatccgca tcacgatgtg aggaaattaa
    30841 catgaatctt aagctgcagc tgaaaatcct ctcttttctg cagttctgtc tgtggggaag
    30901 ttggctgacg accctcggtt cctatatgtt tgttaccctg aagtttgacg gtgcttctat
    30961 tggcgcagtt tatagctcac tgggtatcgc agcggtcttt atgcctgcgc tgctggggat
    31021 tgtggccgac aaatggttaa gtgcgaaatg ggtatatgcc atttgccaca ccattggcgc
    31081 tatcacgctg ttcatggcgg cacaggtcac gacaccggag gcgatgttcc ttgtgatatt
    31141 gattaactcg tttgcttata tgccaacgct tgggttaatc aacaccatct cttactatcg
    31201 cctgcaaaat gccgggatgg atatcgttac tgacttcccg ccaatccgta tctggggcac
    31261 catcggcttt atcatggcaa tgtgggtggt gagcctgtct ggcttcgaat taagccacat
    31321 gcagctgtat attggcgcag ctctttccgc cattctggtt ctgtttaccc tgactctgcc
    31381 gcatattccg gttgctaaac agcaagcgaa tcagagctgg acaaccctgc tgggcctcga
    31441 tgcattcgcg ctgtttaaaa acaagcgtat ggccatcttc ttcatcttct caatgctgct
    31501 gggcgcggaa ctgcagatta ccaacatgtt cggtaatacc ttcctgcaca gcttcgacaa
    31561 agatccgatg tttgccagca gcttcatcgt gcagcatgcg tcaatcatca tgtcgatttc
    31621 gcagatctct gaaaccctgt tcattctgac catcccgttc ttcttaagcc gctacggtat
    31681 taagaacgta atgatgatca gtattgtggc gtggatcctg cgttttgcgc tgtttgctta
    31741 cggcgacccg actccgttcg gtactgtact gctggtactg tcgatgatcg tttacggttg
    31801 cgcattcgac ttcttcaaca tctctggttc ggtgtttgtc gaaaaagaag ttagcccggc
    31861 aattcgcgcc agtgcacaag ggatgttcct gatgatgact aacggcttcg gctgtatcct
    31921 cggcggcatc gtgagcggta aagttgttga gatgtacacc caaaacggca ttaccgactg
    31981 gcagaccgta tggttgattt tcgctggtta ttccgtggtt ctggccttcg cgttcatggc
    32041 gatgttcaaa tataaacacg ttcgtgtccc gacaggcaca cagacggtta gccactaatt
    32101 acgcaaagaa aaacgggtcg ccagaaggtg acccgttttt ttattcttac ttcaacacat
    32161 aaccgtacaa tcgtttcatg ccatccgcat cggtttcgct atagacacct tgcaactccg
    32221 gcgaaaatcc cggcaacaaa ttcacgcctt cttccagcgc aaggaaataa cgttgaaccg
    32281 ccccacccca gacttccccg ggtaccacgc aaagcacgcc aggtggataa ggcaacgccc
    32341 cttctgccgc aattcgccct tcggcatcac gaatccgcac caactccact tcaccgcgaa
    32401 tataagcgct atgcgcatcc tgggggttca tcaccactga cgggaaactc tgctggcgga
    32461 acatcgcttt ttgtaggtct ttgacgtcga aactgacata cagatcgtgc atctcctgac
    32521 acaactggcg cagggtgtag tcgcgatagc gcaccggata tttgttataa acgctcggca
    32581 acacctcagc cagcggcgag tcatcctcaa tatgctgttc aaactgcgcc aacatcgcta
    32641 ccagttgtgc cagcttctcg tggctttccg ccggagttaa taagaacaga atggagttga
    32701 gatcgcactt ctccggcaca atgccgttct cacgcagata gtgcgccaga atcgtcgccg
    32761 gaacgccaaa gtcgctatat tcgccggttt cggcattgat acctggtgta gtgagtaaca
    32821 gcttgcacgg atcaacaaaa tactgatccg cagcatatcc ttcaaagccg tgccacttcg
    32881 cccccggctc aaaactgaaa aaacggcggt cgctggctaa cacagatgtc ggataatcct
    32941 gccacaattt gccatcaaca acgggcggga taaacgggcg gaacagctga cagcgcgcaa
    33001 gaatagcctt gcgcgactca atacccaact caacgcactc agcccacagc cgacgcccac
    33061 tctccccttc atgaattttg gcattaacgt ccagcgcggc aaacagcggg tagaacgggc
    33121 tggtagaagc atggagcata aaggcattat tcaaccgctt atgcgggcaa aaacgcgcct
    33181 gtccgcggat atggttatct tttttatgga tctgcgacgt ctgtgagaat ccagcctgct
    33241 gtttgtgcac cgactgggtc acaaaaatcc ccggatcgtt ttcgttaagt tctaatagca
    33301 gcggcgagct atccgccatc atcgggataa actgttcgta accgacccac gcggaatcaa
    33361 acagaatgta atcacacaga tgcccaacgg tatcgatcac ctgacgggcg ttatagacag
    33421 ttccgtcata ggttcccagc tgaataatcg ccaggcgaaa cgggcgcggc agctcggctt
    33481 tttctggcgc aacgtcgcga atttgctggc gcagatactc ttcattaaaa cagtgcgcat
    33541 caataccgcc aatgaaacca aacgggttgc gtgaagcttc cagatagacc ggcgtcgccc
    33601 ccgcctgaat cagcgcgccg tgatgattcg acttatggtt gttacggtcg aagagcacca
    33661 gatcgccacg cgttaacagc gcattcgtca ccactttatt cgctgccgat gtgccgttca
    33721 gcacaaaata ggttttatcg gcatgaaaga ctttggctgc gaatttctgc gcatctttcg
    33781 ccgatccttc atgaataagc agatcgccca attttacgtc agcgttacac atatcggcgc
    33841 gaaagacgtt ctcaccaaag aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa
    33901 acgcaccatg ttgatgtcca gggcaagcaa aggtgctgtt gcccatctca acgtactgcg
    33961 tcagcgtgtc ataaaacggt ggcagcaaat tctcttcata ctgacaggct gcggattcca
    34021 gctccagcca ctgctgctcg ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa
    34081 cagcatgttc ggaatacaaa aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa
    34141 gaatgccact gcgactatcc gcagcggtaa tgacgactgc cgcgacgtcc gtaaaatcag
    34201 tatctcccaa cgccaccacg cgacgatgag aagaaagtcg ggataccagt tcactactgg
    34261 cggcaatatt cattgatttc ataagcgcaa acccgtttcg gggaagtaag aataccggac
    34321 aaggtggaaa accctgcccc atgagatatg ggtcaaactg gtcaccagct ccgaccgcca
    34381 gacatcagta aaagcagaaa cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta
    34441 acttcaccgc atgagcagta acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc
    34501 gagagaatgt aaggaggtgg cgtgcatcgg gcaaactccg tacagagagg agaaaattcg
    34561 cgcaatcatg gcacctttcg ctaaagcgtg caagccagga ctttgcgacc agacaagcca
    34621 tcaacaaggc aaaaagttca taataagaaa atcaaacaat acagttaaca ggaactcttg
    34681 tggtcatcgg cccttttatc aacgctagtg cagtcttact gggtggcgtt ctcggcgcac
    34741 tgctcagcca acgcttaccg gaacgtatcc gcatctccat gacatctatt tttggtctgg
    34801 catcgctggg gattggtatt ttactggttg tgaaatgcgc caaccttcca gcgatggttt
    34861 tagctacgct acttggcgct ctaattggcg aaatttgttt gctggagaaa ggtgtcaata
    34921 cagcagtcgc caaagcacaa aatctgtttc gtcactcacg taagaagcca acgcatgaat
    34981 cttttattca gaattatgtc gcgattattg tcctgttttg cgccagcggc accgggatct
    35041 tcggggcgat gaacgaaggg atgaccggcg atccgagtat tttaatcgcc aagtcatttc
    35101 ttgatttctt tacggcgatg atcttcgcct gctcgctggg tattgcggtg tcggtaatta
    35161 gtatcccatt actgatcatc cagttaacgc tggcgtgggc tgccgcgctg atattaccgc
    35221 tgaccacacc gtcgatgatg gcagacttca gcgccgtagg cggtttattg ctgctggcaa
    35281 ccggattacg catctgtggc attaaaatgt tcccggtggt caacatgctt ccagcactcc
    35341 ttctggcaat gccgctttcc gccgcctgga ccgcctggtt tgcctgacaa tgcgtgcaat
    35401 agcggcaaag tgatgataga ttgtgcagtc tgcagtaaat tgaagaaatt tgattgacga
    35461 gacgaggcga atcaggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg
    35521 ggattgaaaa tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa
    35581 cggacctcca cggaggtccg tttttcgttt cagaacacca tgatttaagt gttcttcctc
    35641 caaatcaatt ctaccgaact caaccaaatt ctccccacat caacctcatt gtgtgggtat
    35701 aattgcgggc ataccccagt tcgacagaat ttgtaccctc tttaacgcat tcagaaggct
    35761 gaactatggc actgactgac gca
//
LOCUS       DAZFWP020000035        35411 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFWP020000035 DAZFWP020000000
VERSION     DAZFWP020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.035"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..554
                     /locus_tag="ACHK0Z_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /pseudo
     CDS             1..554
                     /locus_tag="ACHK0Z_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            816..2438
                     /locus_tag="ACHK0Z_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             816..2438
                     /locus_tag="ACHK0Z_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4501601.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDPTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2628..2741
                     /gene="yhiY"
                     /locus_tag="ACHK0Z_RS07805"
                     /old_locus_tag="pgaptmp_001561"
     CDS             2628..2741
                     /gene="yhiY"
                     /locus_tag="ACHK0Z_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU4501602.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2804..3871
                     /gene="yhiI"
                     /locus_tag="ACHK0Z_RS07810"
                     /old_locus_tag="pgaptmp_001562"
     CDS             2804..3871
                     /gene="yhiI"
                     /locus_tag="ACHK0Z_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4501603.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3868..6603
                     /gene="rbbA"
                     /locus_tag="ACHK0Z_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             3868..6603
                     /gene="rbbA"
                     /locus_tag="ACHK0Z_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU4501604.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6603..7727
                     /gene="yhhJ"
                     /locus_tag="ACHK0Z_RS07820"
                     /old_locus_tag="pgaptmp_001564"
     CDS             6603..7727
                     /gene="yhhJ"
                     /locus_tag="ACHK0Z_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4501605.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7800..8075
                     /locus_tag="ACHK0Z_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             7800..8075
                     /locus_tag="ACHK0Z_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4501606.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            8072..8431
                     /locus_tag="ACHK0Z_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             8072..8431
                     /locus_tag="ACHK0Z_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4501607.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8481..9341)
                     /locus_tag="ACHK0Z_RS07835"
                     /old_locus_tag="pgaptmp_001567"
     CDS             complement(8481..9341)
                     /locus_tag="ACHK0Z_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HGU4501608.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(9373..9642)
                     /locus_tag="ACHK0Z_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             complement(9373..9642)
                     /locus_tag="ACHK0Z_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU4501609.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(9632..11140)
                     /locus_tag="ACHK0Z_RS07845"
                     /old_locus_tag="pgaptmp_001569"
     CDS             complement(9632..11140)
                     /locus_tag="ACHK0Z_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4501610.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(11133..12491)
                     /locus_tag="ACHK0Z_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             complement(11133..12491)
                     /locus_tag="ACHK0Z_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU4501611.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRITRSTV
                     YA"
     gene            complement(12568..12849)
                     /locus_tag="ACHK0Z_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             complement(12568..12849)
                     /locus_tag="ACHK0Z_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4501612.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(12846..13319)
                     /locus_tag="ACHK0Z_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             complement(12846..13319)
                     /locus_tag="ACHK0Z_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4501613.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(13344..14090)
                     /locus_tag="ACHK0Z_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             complement(13344..14090)
                     /locus_tag="ACHK0Z_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4501614.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(14289..14690)
                     /gene="nikR"
                     /locus_tag="ACHK0Z_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             complement(14289..14690)
                     /gene="nikR"
                     /locus_tag="ACHK0Z_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU4501615.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(14696..15502)
                     /gene="nikE"
                     /locus_tag="ACHK0Z_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             complement(14696..15502)
                     /gene="nikE"
                     /locus_tag="ACHK0Z_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU4501616.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(15499..16263)
                     /gene="nikD"
                     /locus_tag="ACHK0Z_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             complement(15499..16263)
                     /gene="nikD"
                     /locus_tag="ACHK0Z_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU4501617.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRMLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(16263..17096)
                     /gene="nikC"
                     /locus_tag="ACHK0Z_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             complement(16263..17096)
                     /gene="nikC"
                     /locus_tag="ACHK0Z_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU4501618.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(17093..18037)
                     /gene="nikB"
                     /locus_tag="ACHK0Z_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             complement(17093..18037)
                     /gene="nikB"
                     /locus_tag="ACHK0Z_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU4501619.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(18037..19611)
                     /gene="nikA"
                     /locus_tag="ACHK0Z_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             complement(18037..19611)
                     /gene="nikA"
                     /locus_tag="ACHK0Z_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4501620.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(19722..20309)
                     /gene="acpT"
                     /locus_tag="ACHK0Z_RS07900"
                     /old_locus_tag="pgaptmp_001580"
     CDS             complement(19722..20309)
                     /gene="acpT"
                     /locus_tag="ACHK0Z_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU4501621.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(20364..21413)
                     /gene="yhhT"
                     /locus_tag="ACHK0Z_RS07905"
                     /old_locus_tag="pgaptmp_001581"
     CDS             complement(20364..21413)
                     /gene="yhhT"
                     /locus_tag="ACHK0Z_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4501622.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            21545..22762
                     /gene="yhhS"
                     /locus_tag="ACHK0Z_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             21545..22762
                     /gene="yhhS"
                     /locus_tag="ACHK0Z_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4501623.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(22766..23323)
                     /gene="dcrB"
                     /locus_tag="ACHK0Z_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             complement(22766..23323)
                     /gene="dcrB"
                     /locus_tag="ACHK0Z_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU4501624.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(23396..24061)
                     /gene="yhhQ"
                     /locus_tag="ACHK0Z_RS07920"
                     /old_locus_tag="pgaptmp_001584"
     CDS             complement(23396..24061)
                     /gene="yhhQ"
                     /locus_tag="ACHK0Z_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU4501625.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            24282..24527
                     /gene="tusA"
                     /locus_tag="ACHK0Z_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             24282..24527
                     /gene="tusA"
                     /locus_tag="ACHK0Z_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU4501626.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(24629..26827)
                     /gene="zntA"
                     /locus_tag="ACHK0Z_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             complement(24629..26827)
                     /gene="zntA"
                     /locus_tag="ACHK0Z_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU4501627.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(26901..27527)
                     /gene="yhhN"
                     /locus_tag="ACHK0Z_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             complement(26901..27527)
                     /gene="yhhN"
                     /locus_tag="ACHK0Z_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU4501628.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            27668..28027
                     /gene="yhhM"
                     /locus_tag="ACHK0Z_RS07940"
                     /old_locus_tag="pgaptmp_001588"
     CDS             27668..28027
                     /gene="yhhM"
                     /locus_tag="ACHK0Z_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU4501629.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(28030..28299)
                     /gene="yhhL"
                     /locus_tag="ACHK0Z_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             complement(28030..28299)
                     /gene="yhhL"
                     /locus_tag="ACHK0Z_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU4501630.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(28289..28885)
                     /gene="rsmD"
                     /locus_tag="ACHK0Z_RS07950"
                     /old_locus_tag="pgaptmp_001590"
     CDS             complement(28289..28885)
                     /gene="rsmD"
                     /locus_tag="ACHK0Z_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU4501631.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            29035..30528
                     /gene="ftsY"
                     /locus_tag="ACHK0Z_RS07955"
                     /old_locus_tag="pgaptmp_001591"
     CDS             29035..30528
                     /gene="ftsY"
                     /locus_tag="ACHK0Z_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU4501632.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            30531..31199
                     /gene="ftsE"
                     /locus_tag="ACHK0Z_RS07960"
                     /old_locus_tag="pgaptmp_001592"
     CDS             30531..31199
                     /gene="ftsE"
                     /locus_tag="ACHK0Z_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU4501633.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            31192..32250
                     /gene="ftsX"
                     /locus_tag="ACHK0Z_RS07965"
                     /old_locus_tag="pgaptmp_001593"
     CDS             31192..32250
                     /gene="ftsX"
                     /locus_tag="ACHK0Z_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU4501634.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLLSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            32495..33349
                     /gene="rpoH"
                     /locus_tag="ACHK0Z_RS07970"
                     /old_locus_tag="pgaptmp_001594"
     CDS             32495..33349
                     /gene="rpoH"
                     /locus_tag="ACHK0Z_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU4501635.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            33620..34723
                     /gene="livJ"
                     /locus_tag="ACHK0Z_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             33620..34723
                     /gene="livJ"
                     /locus_tag="ACHK0Z_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU4501636.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(34922..35305)
                     /gene="panM"
                     /locus_tag="ACHK0Z_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             complement(34922..35305)
                     /gene="panM"
                     /locus_tag="ACHK0Z_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU4501637.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         8646 a         9013 c         9483 g         8269 t
ORIGIN      
        1 aactatactg catgactaga aaattataaa ctctctgccg gtgatacgta taacttaatt
       61 aaaacgctcg atgtttgtca ttatgactca gaaaggaaag ttttaaccag tcatcacgga
      121 ataataagag atgaagaaaa aaatgttatt gtctcggtgc tttgcaggtt ccatttaatc
      181 agatgaaaaa tcctactgat cctgaggaat tagccaatat ttttaataaa aaacataaag
      241 aacacatgga tgatcctcta ttccatctaa tccgaagcaa tactttgaaa cccacacctg
      301 tatatgctaa ttattttgat aacactactg atttcagacc agcacgtaaa cgtatcttca
      361 tatatgggga gacactcaaa ggtgaagacc ccagcaaata cattagacaa aaatatgggc
      421 atcatggggt cggggtagat cagaatcaac aatttgataa tggtattatg gggctaaatt
      481 ccctgaagga agcacgtgat aaaaataata aaataattta ttcctcaggg cttagttgtt
      541 ttcaatctca ttaaaactcc atgaatacgg tggtatgtct ttttaatcct gccagtattt
      601 cataggtttt cttaatcgat tgtagaggca tgttctgatg acgcctctac aatctttagg
      661 gactttctct gctatgagat aagaaatttc atattatgct ttaaggctga atgatttgtg
      721 caaaatataa ttatttttcc atcgaacatg aggtgtatgt cataagatta aatgttaact
      781 atgcattcct cgcggtgaat tatatgaggt aaaagatgaa aattggaact gtggcaggca
      841 ctaacgacag tacaacgaca atcgcaacaa atgatatggt gcaagagcat gttactaact
      901 tcactaaaga actgtttggg tatattgcaa atggtatagg tgatgatata tcaagtattg
      961 ccaggacaat gctcggtgaa gttgtggaaa aaattgatga ttggcaaata gaacgtttcc
     1021 aacaatctat tcgagatgac aaaatatcat ttaccataca aacgaatcat tcagaaaaat
     1081 atagtatgct atcggacatg cgcgctcata ttctacgtag ggataataat tatcagttta
     1141 ttgtgacaat taatagtaaa aattacggct gctatcttga taataccgat attaattggt
     1201 gcagcattgt ttatttactt aataatatga cagtaaatga tgatgctaat gatgttgcag
     1261 ttactgagtc gtacaaaccc gtctgggact ggaaaatatc gcaattcaat gtatctgata
     1321 ttaaattcga aacaatgata aacccccagt tcgctgacag aacctatttc tccaattgtt
     1381 cacctgttga tcccacaagt accagaccaa cctattttgg cgataccgat ggctcagtcg
     1441 gtgcagtttt gtacgcgctt tttgcaacgg gacatttgag aatcatggct gaaggagaaa
     1501 atttccttag ccagttgctt aatatagaag atgaagttct caacgtattg ctaagggaga
     1561 actttaatga gcaacttgac actaatgtta atacaataat cagtattctg aacagaagag
     1621 atatagtttt agaaagtctt cagccttatt tagttattaa taaagacgct gtgacgccat
     1681 gtacgtttct tggcgaccca accggcgatc gatttagtaa tatctgtggc gatcaattta
     1741 tcatcgattt gttaaaacgc ataatgagta tcaatgaaaa tgttcatgta ttagctggaa
     1801 atcatgaaac gaattgcaat ggtaactata tgcagaactt cacgcgcatg aaaccacttg
     1861 atgaggatac ctacgctggg ataaaagatt atcctgtatg tttctatggc cccaaataca
     1921 aaataatggc aaatcatcat gggataactt ttgatgatca gcgaaaacgt tacattatag
     1981 gaccgataac agtctctagt gatgaaatga ccaatgctct cgatccggtc gaattagcgg
     2041 caattatcaa taaaaagcac catgctgtaa ttaatggcaa aaagtttaaa aatagccggg
     2101 ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac agacctaaac
     2161 ttgaagcact attagcatgc tcacaaatgt taggtataaa tcaagttgtt gcacataacg
     2221 gtaatggtgg aagagaatgt ataggtgaaa cggggacagt tttaggtctt aacgcccgtg
     2281 acagtaaaca tgctggaaga atgttcagca tgcataattg ccaaattaac cctggtgcag
     2341 ggcctgagat aaccactccc tggaagtctt accagcatga aaaaaacaga aatggactta
     2401 tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac atgaatatta
     2461 atgttactat ctggttgaga ctgttgataa aacgtaaaaa ggaatgctct gttcctgcaa
     2521 ctgaacaggc gtgaaacgcg atccaacagg atggataccg ttttgtcagc cagttttata
     2581 gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat
     2641 tactacccgt tttcactaag ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta
     2701 tttgtcgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt
     2761 gtaacacgcg tcgtttgctt acagggatta tggcgggccg attatggata agagtaagcg
     2821 ccatctggcg tggtgggttg tcggggcact ggcggtggct gccgtcgtgg cgtggtggct
     2881 gttgcgcccg gcgggtgtgc cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac
     2941 ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg tgaaagaagg
     3001 ccagtttgtt cgcgaaggtg aagtgctggc gaagatggat actcgcgtgt tgcaggaaca
     3061 gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc gccgttgccg ccgcgcaggc
     3121 tttgctggag caacgacaaa gcgaaactcg cgccgcgcag tcgctggtta atcaacgcca
     3181 ggcagaactg gactccgtag caaaacgtca tatgcgttcc cgttcactgg cccaacgagg
     3241 ggctatttct gcgcaacagc tggatgacga ccgcgccgcc gctgagagcg cccgagctgc
     3301 gctggaatcg gcaaaagctc aggtttctgc ttctaaagcc gctatagagg cggcacgtac
     3361 caatatcatt caggcgcaaa cccgcgtcga agcggcacaa gccactgaac ggcgcattgc
     3421 cgcagatatc gatgacagcg aactgaaagc cccgcgtgat ggacgcgtgc agtatcgggt
     3481 tgccgagcca ggggaagtgc tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag
     3541 cgacgtctat atgaccttct tcctgccaac cgaacaggcg ggcacgctga aactgggtgg
     3601 tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt attccggcga ccatcagttt
     3661 tgtcgccagt gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa
     3721 actgatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata
     3781 tgtcaaaacc ggtttaccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc
     3841 tgacgacctc gtggtgaggt tgccgcaatg acgcatctgg aactggttcc cgtcccgcct
     3901 gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatt
     3961 actctcgata ttccggcccg ctgtatggtc gggctgattg gtccggatgg cgtcgggaag
     4021 tcgagcttat tgtcgttgat ttccggtgcc cgcgtcattg agcagggcaa tgtgatggtg
     4081 ctgggcggcg atatgcgcga cccgaggcat cgccgcgacg tctgcccgcg catcgcctgg
     4141 atgccgcagg ggctgggcaa aaacctctac cacaccttgt cggtgtatga aaacgtcgat
     4201 tttttcgctc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat taatgagctg
     4261 ctgaccagca cggggttagc accgtttcgc gatcgtccgg cagggaaact ctccggcggg
     4321 atgaagcaaa aacttgggct gtgctgcgcg ttaatccacg acccggaact gttgatcctt
     4381 gatgagccaa caacgggggt tgacccgctc tcccgcgccc agttctggga tctgatcgac
     4441 agtattcgtc agcggcagag caatatgagc gtgctggtcg ccaccgccta tatggaagag
     4501 gccgaacgct tcgactggct ggtagcgatg aatgccggag aagtgctggc aactggcagc
     4561 gccgaagagc tacggcagca aacgcaaagc gctacgctgg aagaagcatt tataaatctg
     4621 ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa
     4681 aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc
     4741 gttgatcacg ttaatttccg cattccacgc ggggagattt ttggattcct tggttcgaac
     4801 ggctgcggta aatccaccac catgaaaatg ctcaccggac tgctgcccgc cagcgaaggt
     4861 gaggcgtggc tgttcgggca accggttgat ccaaaagata tcgatacccg ccgtcgggtg
     4921 ggctatatgt cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca aaaccttgag
     4981 ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcaagagt ggctgaaatg
     5041 agcgagcgtt ttaagctcaa cgacgttgaa gatattctgc cggagtcatt gccgctcggc
     5101 attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggagat gttaatcctc
     5161 gatgagccta cttctggtgt cgatccggtg gcgagggata tgttctggca gttgatggtc
     5221 gatctctcgc gccaggacaa agtgactatc ttcatctcca cccacttcat gaacgaagcg
     5281 gaacgttgcg accgcatctc actgatgcac gccggaaaag tgcttgccag cggtacaccg
     5341 caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat cgcctatttg
     5401 caggaagcgg cagggcagag caacgaagcc gaagcgccgc ccgtggtaca cgacaccacc
     5461 cacgcgccgc gtcagggatt tagcctgcgc cgtctgttta gctacagccg ccgcgaagcg
     5521 ctggaactgc gacgcgatcc agtacgttcg acgctggcgc tgatgggaac ggtgatcctg
     5581 atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgctgtgctc
     5641 gaccgcgacc agaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac
     5701 tttatcgaac agccgccgct caccagttat gacgagcttg atcgtcggat gcgtgcgggc
     5761 gatatcacgg tggcgattga gatcccgccc aatttcgggc gcgatatcgc gcgtggtacg
     5821 cctgtggaac tcggcgtctg gatcgacgga gcgatgccca gtcgtgccga aacggtaaaa
     5881 ggttacgtgc aggcaatgca ccagagttgg cttcaggatg tggcgagccg acaatcgaca
     5941 cccgccagcc aaagcgggct gatgaacatt gagacgcgtt atcgctataa cccggacgta
     6001 aaaagcctgc cagcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca
     6061 atgctaagcg cccttagcgt ggtgcgggaa aaagagctag ggtcgattat caacctttac
     6121 gtgaccccca ccacccgtag cgaatttttg cttggcaaac agctgccgta catcgcgctg
     6181 gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat
     6241 aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat tgccaccgga
     6301 atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt cggaacggcg
     6361 attatcacat tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg
     6421 gaagggcctg gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc
     6481 cgcgggacgt tctcgaaggc gctggatctg actgatttgt ggcaactttt tatcccgttg
     6541 ctgatagcca tcccgctggt gatgggctta agtatcctgc tgctgaaaaa acaggaggga
     6601 tgatgcgcca tttacgcaat atttttaatc tgggtatcaa agagttgcgc agtctgctcg
     6661 gtgataaagc gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag
     6721 cgaccgttac gcctggatcg ttgaacctcg cgccgatcgc cattgccgat atggatcaat
     6781 cgcagttatc gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga
     6841 tgatcaccgc cgatgagatg gatgccggac tggacgccgg acgctatacc ttcgcgataa
     6901 atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccggat attcaggtga
     6961 acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta
     7021 tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat
     7081 cgctggaaac ccggatgcgc tttaacccga accttgatcc cgcgtggttt ggcggggtga
     7141 tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc
     7201 gcgagcgtga acacggcacg gtggaacatt tactggtgat gccgataacg ccgtttgaga
     7261 tcatgatggc gaagatctgg tcgatggggc tggtggtgct ggtggtatcg ggattatcgc
     7321 tggtgctgat ggtgaaaggt gtactgggcg taccgattga aggctcgatc ccgctgttta
     7381 tgctgggcgt ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt atggggacga
     7441 tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgcta ccgttgcaaa
     7501 tgctttctgg tggatccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc
     7561 tgaccatgcc gacgacacac tttgttagcc tcgcgcaggc catcctctac cggggtgccg
     7621 gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc gcgtttttca
     7681 ccatcgcact gctgcgattc aggaagacga ttgggacaat ggcgtaagga agggaaactt
     7741 gacttcatac tgtgatgtca tactattgat gtcataaagt gaagtcataa ggacatgcca
     7801 tgagaaccaa ggtacaggct ttacggaaga aacaaaaaaa tacattggat caaatattta
     7861 aaactcctgt tcctcaaggg atcaagtggt ctgatataga gtcactggtt aaagcattag
     7921 gcggagaaat taaggaagga agaggttcgc gttgtaagtt catactaaat atgagcgttg
     7981 cgtgtttcca tcggcctcat ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc
     8041 gtgactggtt actaagtata ggggtaaaac catgatcaaa ctgaagacgc caaactctat
     8101 ggagattgcc gggcaacctg cagttatcac ttacgtgcca gaactgaatg catttcgtgg
     8161 caaattcctg ggcttgtcag gttattgtga ttttgtctct gacagcattc aggggctgca
     8221 aaaagaaggg gaactttccc tgctagagta tcttgaggat tgtaaagctg cgggtattga
     8281 gccgtatgcg cgtacagaaa agattaagac ctttacgtta cgctatccag aatcgctgag
     8341 tgaacgcctt aataatgcgg ctgcgcagca acaagtctct gttaatacct atattattga
     8401 aacactgaat gagcgtttga atcatttata acttatagcg gcccaacaat ttctgttgcg
     8461 ccgctatatt tttgtggctc ttaagatgcc tttctaacca accgatgccc ctcattcatc
     8521 atcaggattt tgctatacac atcttcctcg acggcgtttt ttgctgatgc catgactcgt
     8581 gccagattcg cctcgttgtg attattcaca tacgctttgc cgacggcggt gataaacgct
     8641 ttgcgcagat cgctggcgat atttacttta gcgacgcgat aattaacaaa gctgcgtaat
     8701 atctccgggg cgataccgga gccgccgtga ataaccaacg gcaccggaca gacttcggca
     8761 atgcgcttca gcagggggat atcgatacgc ggtatatcgt ccaggccatg aacgttgcca
     8821 atagaaactg ccagcatgtc gcaaccggta cgctcgacaa aggtcttcac cttttccggt
     8881 tccgttttgc aatcggcttc gctgacatga tcatcttctt tgccgagaat cgcccccagc
     8941 tccgcttcaa ccggtacacc ataggattta cagaaatcga cagcttcctg ggtgaaagcg
     9001 atattctctt caaaagagaa tgcggcaccg tcaatcatga ccgaggtaaa gcccgcgcgc
     9061 accgcctgtt tcacatcctc cagagttttc ccgtgatcca gatgaagact gaccggaaca
     9121 tccattttat cggcgtgacg tttcacgatt tcataaatgt aatcgtatcc cgagagttgt
     9181 gcattggttg gcgcaatctg aatgaagttg ggtaatcccg aacgctcaat agcgtttaaa
     9241 attgaaattg tcgtctccag attggtggta ttaaatgcgc ccgctaatac ccgtttttcc
     9301 tgaatgcggt cgagcagtat ccgtccatta actaatggca tattgaaaac ctcatctttt
     9361 aatatcagtt aattattcag cttcaccgaa gcggtcgcga aataagtcgg tgagagtctg
     9421 tatggcctcg ttttcatcat caccgtcgca gcgtatttcc accgtttttc cgccttttat
     9481 tccggcgcgc ataatattca tcatgctggt ggcattaata tcattaccct catagtgcag
     9541 cgtcacttta cagctatatt ttttggcggc tttcgccaga caggccgccg gacgtgcatg
     9601 caggccagta ctgtttctga cctccacggt tttattcaac atagtgttcc tcccgatgat
     9661 gtaatggcat cacgccttct tgttgtaatt tcaactgata ccagcttttc catttgtgct
     9721 gataacgctg taattgatgg tatctcgccc gattcggtaa caaacgttgg gctatagcga
     9781 ctggcggcgg tggaatatcc ggtgcggcgg ttaataatgc tccgacagaa accgcctcgt
     9841 taaatgcaat tgcttcaacc ggacgctgta aaatatccgc ctttaattgc agccaaagag
     9901 gattatgggt tgccggaccg atcactttta acgcgctggt gccaggcacg ttaaaacagg
     9961 cgaagcagtt ggcaaattcc atggttaatc ccagtgatac gctggcaatt aacatgtcga
    10021 tacttaacgt atcgccaagc ccataaaata atccacgggt atggcgattt ttatagggcg
    10081 aacccgaacc gcgaagatgt ggaataaaga cgggaatgtc atcgagcaac caattccccg
    10141 ccagataatc agcatgtccg cgagtaagtg atgcggcgat ctcctcatcg gttagccgga
    10201 acgtgttgcg cagccactcc agcgcgaaac cgcccacggg cagcgacgca aacagggtga
    10261 actggttggc aagtgagtaa cagccgtttg ccagcttgtc acgtttggcc tgttcatcca
    10321 gcgtcggttg tgtatccagt tgcagaatgc cttccgtggt ccccgtcgag ttaaggatat
    10381 cgcctggcat catctgcaac gctcgcgcac ccaccatatg gtcatgtccg gccagcgtca
    10441 cgctgaccgg ttgcgagaac ccaagcgtct tgcaaagtga ctcgctgacc catccggcgt
    10501 gctcgcctgg ctgcaccaat ggcgcaaatg ccgtacaggg aacgtgtaac aacgccgccg
    10561 ctttcgctga ccattcgcct ttgcgcacgt ccagacacag cgtgcggctg gctaaggtga
    10621 tatccgtgcg ctgttcgcca gtcagccgcc agagcagcac ttccggcgca tgtagccagc
    10681 gcaggccgcg gcgattacgc agcgggtaat gttccagcaa ccagcgcatt ttgaaggcag
    10741 agtaattgct gtgtagtggt agaccgcaga tgtcataaag tgctgcactg tctgcctcgc
    10801 taagcgttgc cagatactct tcaccgcgac ggtcatacca tgccagcatt ggcgtcagga
    10861 tctcgccatg ctcgtcaagg aacacgcctg attcgccaaa actggcaatg ctgatccgtc
    10921 tgactggcag cggcgaagcg gcgttcagtt gcgctatcgc ctggcggacc tcctgccaga
    10981 gggcgtcgat atcgaaatcg acatcgccct gtggggagat ctgctttgcc gtcacgaatt
    11041 tatgcgcgcc caacgtcgtt gcgtccaggc aggaaaagca ggtgactttg caattggtgg
    11101 tgccaatatc gataacgata gctgcgctgt tatcaggcat aaaccgtgct ccgcgttatt
    11161 cggaaaatgc catacagtac cagggcgata accggcacca gaccaatggc gatgatattg
    11221 ccgttgaaga tttccagcag ccagaagcgg aacgggttac cgccatcaag gaaactggaa
    11281 acctggccgc tggagccttc ggcaaatttg aagttaacgt ctttcgccat gccggtaata
    11341 aacggtgcga ttttagtcgc aatccacagg tcggcaataa tcaccgggat cgccgccaga
    11401 acggtgcgga aaatattgcc acggctggct aaagcaatca tcgacgccat taccgccagg
    11461 ttggcaaggt cgccaagcgg caggacttca ttacccggaa ggacaaacgc gataaagacc
    11521 gagataggcg tcagcagcag ggcggtggag ataatcgccg ggttacctac ggcaacggcg
    11581 atatccaggc cgatatagag atcgtcacgg tcagggtatt tggcattgag atacttttta
    11641 atggcttcag acagcggtaa taaaccttcc atcaggatgc gtaccatgcg tggcaggatg
    11701 aacatcacac cgccaatgct gatgccaagc agcaacactt ttttgaaatc gtatcctgca
    11761 atcacgccga gcagtatgcc cagaatagtg ccaaccatca tcggttcgcc gaagatgcca
    11821 aactttttct gtacggtttc cgggtcgata tgaatacgat tgaggccggg gatatggtcg
    11881 atgattttgt cgaccagcag accgaccggg aagaacacca ccgaagagag cgtcggcaat
    11941 gagatccctt ccagtccaaa gtatttttgt accagtggcg cagaccagtc ggcgagttta
    12001 agtactacca ccgcagcaat cgccgccgcc agcaaaccaa agaacaggct gccggtgctg
    12061 taataaacca acgtaccagc aagggcaaaa tgccagtagt tccacatatc tacgtcaacg
    12121 gtacgggttt tatttaacgc cagcatcgcc acgttaatca gaatggtcag ggggataaca
    12181 aacggggcaa ttggcgaacc ccatgtaata gcggaaagcg gcggccagcc taaatctacc
    12241 accggtaaat taattccggt gcgttcaacc atcgcctggg ccgctggccc gacattgctg
    12301 gtcagaatat cgaaaatggc ataaataccg acaaaaccaa tgccgactgt taatgacgaa
    12361 cgtaaggctt tagcgggttt tactttaaaa cagagagcaa gaataaataa caccaacggc
    12421 aacattaccg tcgggccaaa tcccaatata tattgcacaa tattatagag ggtatgcgcg
    12481 atatcgttca taataaacct ctttttcagg cgagagcgca cctcgcgtta gcgcctgatg
    12541 gagtgggttg aaattaatta ttattaatta atttttaagg atcgaaacaa tttgcgccag
    12601 tactttctct tcgccaatac cggtaataat cggcaagccg ttaactaccg ggacgtcgag
    12661 ttcataaggc actttagtcg ttgagactac cagtgctacg ccatcagtat ttccaggtaa
    12721 agaggcaacg ttggtttggg aataattgac gttaagaccg tgctctttac aatattccat
    12781 gactttttct gttaccgccg tggatgtggc aatacccgtg gcgcagacaa ataaaatggt
    12841 ttgactcata tttcacctcg ttctttcatt ttgtttaaga ttactgtgtg tatttctgtt
    12901 gtttccatcg caagcagggc ttgcataaag ggcgtatcct gaatcatttc cataatccag
    12961 cccagggcat ttaattgttt attactttcg cttaaggcga gcaaaaaaat caccctgaca
    13021 ggtacggggt caggatcgcc gcccatatcg gcaaactcta ccggttccgg caggatgccg
    13081 acggcaattg cattgtgcct gacatgttta tggtccgtgt gcggaatagc gacgcacggt
    13141 ggttcggtag gcagccccgt tgggtacgtc gcctcacgtt tcagtacgtg atcggcaaaa
    13201 tcagggacga ccagctcggc ggcaaccaac ggttgcgcta actgacggat gatctgttca
    13261 acgcttaacc ctgccggaag tcgttgaata taattttcct gaaaatgtat gccttgcatt
    13321 ttactcctct taaaaaagtg tacttaactc acttcccgcc gttgcagtac ggtgccaaga
    13381 taatatttat ttccttttaa ccagacgttg ccaaactcaa tgggtaatcc tcgggagaaa
    13441 aagactaact gctccagatg caaaacgggt gcgtcttcac tgatatctaa aaaatgacca
    13501 cgttcattac caattaatcg cgcggcatag cgactttccg agtaacgaat ttttctttgc
    13561 gacaaacttt ctattgttgg aaataaattg tgttgattaa aatcgatttc cgcaataccg
    13621 gggcatagct caatattgat acggttctct atcagcatcg ctttttcatc accaattgag
    13681 cgtaagcgtt caaggtaaag aatatcctga ccgggcgtta ttcttaattt ctctgccaca
    13741 taacgattag ccgattcaat ccgtgacgta ataacttcag tggtaaaatg tattttctgg
    13801 ctttccagcg attccgcgaa tgacaataat ccttcaccta acggatatgc cacgttctct
    13861 tttttgacaa atgttccctt accctggacc tgtattaaca cgccttcttt taccagttgg
    13921 gcgaccgctt ttttaacggt tccccgactc acttccagca tatccataat ctggtattcc
    13981 gaaggaatgc gatcgtcttc caccagatct ccggtataaa tactttctct tatccactcc
    14041 accacttgtt gatagagcgg tatttgtgac gacttactta atgatttcat ggcgcgcacc
    14101 tgaacattta cagttgtcta tggacaatca tggacaactt caagcgtata gtaggtgaac
    14161 gggagaaaaa gaggatcgaa gcgtctcaca ttttgaggga ccaaatcgtg atcaaaaaaa
    14221 tgtgacgtga gtcgatattt gtcagaagtt tactgcgccg taaggaaata acggcgcgtt
    14281 ggtcggtgtt aatcttcctt cggcaagcac tgcaaatgcc cgtgccgcac gccgcgctgg
    14341 gcgataacgt catcggcaaa atgctgcacg tcacccatgt cacctttcaa cacggcgatt
    14401 tccagacagt cgtcgtggtt gatgtgcaca tgcagcgtgg cgacggagag gtcgtggtga
    14461 tgatgctggg tggaaacaat acggctggct aagtcgcgtt tttcgtgttc atacacatac
    14521 gacagcaccg cgaaaccttg tgtgccgtgc tgctgggtag cttcttgcgc cagggcgcta
    14581 cgcagaatat cgcggatagc ttcggaacgg ttgttataac cacgacgctg gctcaggctg
    14641 tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat ttgcgttaaa
    14701 ccttttctgt ggtgcgacgg cgcacgggga atgcgggtaa taccgcgttt tgtagcacac
    14761 gtccggcgtc agaggaaaag gttaatttat ctcccaccac ctgggtttcg acgatttgtc
    14821 cgttgtccat caccatcacc cgctgacaga agcgttccac caggcgtaag tcgtgggtga
    14881 tgaacaggca ggcggtgcca aactgttgtt gtagcttttt cagcaggcga atgacgcccg
    14941 cctgtaacac gagatcaagg ttagaaacgg cttcatccag aatcagtagt ttcggttcga
    15001 ccgccagcgc gcgagccagg cagacgcgct ggagctggcc gccgcttaac tgcggtgggc
    15061 gtttgtcgag aacgctgtca tcgagatcga ccgccttcag catttcgctg gcgcgcgcca
    15121 gttgttcgga ttttttcagt gatagcaggt ggcgcatcgg ttcacgcagg atctcgcgca
    15181 cggttttgcg cggattcacg gcgctgatgg agtcctgaaa taccatctga atatcgcggc
    15241 ggaacgcttt acgctgggcg cgattgagtt tcgccagcgg ttcgccgcgc cagctaatat
    15301 tcccttgtga gggagactct aagcctacca gcagccgcgc gagggtgctt tttccgcagc
    15361 cgctgcgtcc cagcagggcg acggtttcgc cgcttttcag ggtcagggaa acgttattca
    15421 gcaccgcctg atgttgatgt tttccgctaa atccaccgtg ggcatagtga tgggaaaggc
    15481 cgcagacgtt aagtaaggtc atgatgccag ctccataccg tagagggcga gatgagcgga
    15541 aaccaggctg cgcgtcaccg tatgtttggg ggcgttaaac agcgtttcta catcgccctg
    15601 ttcgacaatt ttaccttgtg acatcaccgc cacgtcatcc gccagacgcg ccacaacgcc
    15661 catatcatgg gtgaccagca gcattcccgg cgcttgtttt tgcataatgc tttccagcag
    15721 atcgaggatg cgcgcctgtg ctaccacgtc gaggtcggtg gtcggttcat cggcgatgat
    15781 aaacggtgat tcacacagca ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct
    15841 catctcgaac gggtacagct tcagcacgcg cgcggcgttt tccagcccca ccgcttctat
    15901 ggcagcggta agcgtagcgt catcggcggg tttccctaac gccaggcagg tttcccgcgc
    15961 gtgggtgtgc atggtgtgca gtggattaaa ggcgctgcgc gggttctgca tgatggtggc
    16021 aattttgatg ccgcgcaggg cgcaaggcga aaccggtttg ccatcggcta aaatttcccc
    16081 cgccgtctgg cgaacgccag cgggcaaaat gcccagcgtc gcggcgcagg ttaatgattt
    16141 cccgctgccg ctaccgccga ctaacgccag catgcgcccg cgttgcaggg ttaacgatac
    16201 accgtgtacc agcggctgcg cggcctgtag cgcgatatta cgtagttcaa tctgttgcgg
    16261 cattagtgtg cgtgctccgt caccagatga gggtccagat gatcgcgcag tgcgtcaccc
    16321 accaggttaa aggccatcac gctgataaac agcgccagcc ccggccagaa catttgcagc
    16381 ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc
    16441 gcggtcacac cgaggccaag gaaagacatc cccgcgacgt gcagcatcat atggccgata
    16501 tccagcgttg ccagcaccag cagcgaaggg atcaccgcgc ctgccagatg atcgacaaac
    16561 acccgcacat ggcccgcgcc agaaagccgt gacgccagca caaactcgcg ttggcgtagt
    16621 gaaatcacca ggctgcgcac catgcgtgca taccacgccc agtgcgacag ggcgatggcg
    16681 ataattacgt tggtcagccc ggtgccgagc acgccaacca taaagaacga cagaatcgag
    16741 gtcgggaagg tcataaacat atcggcgacg cgcatggtgg cctgatcaac gcgcccgcca
    16801 atcaacccgg cgctgccgcc aataaccagc cctaatgtca gcaccagcag caggcaggcc
    16861 attaccgaac cgagcgacac gcgggtcgct gccatcagcc gcgagaaaat atcgcgacct
    16921 aagtgatcgg tgcccagcca gtgctgcgca tccggcgaaa gcaggcgcga cggcaaatca
    16981 atcgcctggg ggtcatacgg cagccaccac tggctggtga gcgcaatcag cgccagcagg
    17041 gcgataatga tcagcgccag gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt
    17101 gcgctccttc atgacgacga atgcgcgggt ccagcgcggc gttgagcaaa tcgacaatca
    17161 aattacagac cacaaaaacc accaccatca tcagcgtaaa gcactggatc accggatagt
    17221 cacggttaaa aatcgccgac accgcatagc gcccgacgcc cggccaggca aagatgtttt
    17281 cgataatcat cgtcccgcca atcagttcgc cgatgtgcat ccccacggcg gtgatcatcg
    17341 gcagcgaggc attgcgcagg atgtggcgac gttcggtctg tttgtcgttc aggccgcgca
    17401 gacgcgccca ggtgacgtga cgctgaccgg cgacgtccag catactggcg cgcagtaaac
    17461 gcgcgttaat cgccagcgac ataaaggcaa tggaaaccgc aggcaaaatg atgtgctgcc
    17521 agccgccgta acccatcgcg ggtagccatt gcagatacac cgaaaacgcc atcaccagta
    17581 aaaacgccag ccagaagtta ggcatcgaca cgccaagaaa cgcgatgaaa cgtacggcga
    17641 aatccggcag acggtcgcga tggcgcgccg cccagatacc gagcggtacg gaagtgagca
    17701 gaattaatac cagcgccgca cctgcaagtt ccagcgtggc gggcaggaag ttcagcatat
    17761 cgtccagcac cgggcgttgg ctggcgaatg agataccaaa gtcaagatgc agcgccttcc
    17821 acaaccaggt gccgtactgg acgtacagcg gctgatccag cccgagcatg gtgcgggtag
    17881 aggccagcat ctccggcgtc ggcggcaggt tagataaacg cagataatcg agcgccgggt
    17941 cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga ggcggcgagc accatcggga
    18001 tcagcagcag aaagcggcgt aatacgtaac gcaacattaa ggtttcaccg gtttaatctg
    18061 ttcgaacgga atttcagtgg cgatcggcgc gtaggggatg ttacccagct ccggttttga
    18121 taccaccatc attgagatgt aactgatagg cagataaacc gcctcgtcat gcagacgggt
    18181 cagaatgtcg cgatacagcg cctgacgttg cgtttcgtca tgggtcgcca gcacttcgcc
    18241 gatctcttta tcaatcagcg gtttgtcggc taatccttgc tgtgcctgga agtcagcgtg
    18301 tgacggtacg cgcattgaac tgaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg
    18361 gtggaaaatc atgccaaaac gaccgtcgcg ctgacgagca tagatactgc tctcttcttc
    18421 gccaatcagc gagacatctg cgccaatctg gcgcatatca gcctgaatga tttccgccat
    18481 cgatttgctt aacgcatcgg tgccgatgaa cgacagttca atgcgcagcg gctgaccatt
    18541 tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg gctttttcca gcaacgcttt
    18601 cgctttctgc ggatcgtact ggcgcggttt caggccgagg ttggcgtagg gcacagaagg
    18661 ggcaaacagg gtgtcggcga cctgctgggt gccatacaac gcgttatcaa tcagcgattt
    18721 tttgtttacc gcgtaattaa gagcttcacg tactgccagc tcattggtgg gggctttggc
    18781 ggtattgagc gccagcatca cggtttcgat cggctgtgac agttgggtgt ggtaagccgg
    18841 attctggcta aagcgggcga aggtatcgag cggtaataac ccttcgtttc cgtacagcag
    18901 gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc gggtccggga tgacgttaaa
    18961 ggtgatcttt ttaatcgctg gcttttcgcc ccagtagttt tcgttacgga cgaagacatc
    19021 gtactgattc agtttcgatt cctgcaaaat ccatggtccg gtgccaatcg gcgctttaat
    19081 tccgttcatg gtttcatggt ttttaaactg cgagggagcg ataaaacgga aaggacgggg
    19141 cagggccagt tcttgcagga aaggatagta ggcgcttttc agggtaattt gcagctctgt
    19201 tttactgagt gctttaacat caacaatctg gtttgccagc tccagccagg cgtgacgttg
    19261 acggttatcg agcactgcgc ggaagttttc tgccgccgcc tcggcatcga acggttcacc
    19321 gttggagaat ttcacgtcat cacgcagggt gaaggtccag gttttaccat cttctgaatg
    19381 agtccagctt tttgccagcc acgggatcac cgaaccgtct gcctgatatt tcaccaatgg
    19441 ttcataaacc atgctctggg cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg
    19501 cccgacattc accggccagg cggtagtgat ttcatctggt gcagcggcat ggacgataaa
    19561 agacgcacaa gccagcagcg caaatagagt gcggcggagt gtggagagca tggtaacccc
    19621 aatggattaa aatagatggc ggaaataagt atgacgattt taagtattcg tcatactgat
    19681 tacctgttct ggatcaatag atgggcagtc gggtgggcgg atcagttaac tgaatcgatc
    19741 cattgcacac tgtcggcggt gagcgtaaag ggggtggggg tgcagatcgc caggcttaaa
    19801 ttttcaagct gacaatggct gactgacagc gaggagtgat aggtgctgtc tacgctgacg
    19861 atttgccagg cgctgccgcc acgctgttta acgatggctt ctttgcgcgt ccagatgcgc
    19921 cagaacattt ccagttgctg atcaggatgc acggcgtcca tctcagcgtg ttccccgagg
    19981 ctgaatacgg cgttcgccag ccagcgccag ttggcgcgcg ggcgaatcac ttcgatatcg
    20041 cagccgactt cgccttcatc actcaacagc agagcgatat cgtcaccgct atggcttaag
    20101 ttgaaccata gcggcgtttc cggcgcgaat gcaggtttgc cttgttcgcc atagatgatc
    20161 tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgcc ccgccagcca gcgttcgcgt
    20221 cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc
    20281 gaaactttcc ccagaactat ccgatacata tcagggccaa cgtttaatgg aaaatgaaag
    20341 tgcgtatcgt atcacttgtc gcctcatccc ggtaaccgac ttttcggtct gcccggcccc
    20401 agtaaaatcg ccagtttgct gccacctttc gtcgtttcca tccagatttt acacacgctg
    20461 gttaatggca ccgaaagtag catccctacc gggccgagca gccatcccca gattaacaat
    20521 gaaagaaata ccaccatggt ggacatcccc aggcgatggc ccatcatccg tggctctaaa
    20581 atattgccga tgaccatatg gacaactaaa aacaatgcgc cgaccagaat acattcgtaa
    20641 ataccattaa acagcagcac ctgaatcatt ggcggtacgg cggaaattac cgcgccgata
    20701 ttgggcacgt agttaagcaa aaacgccagt actgcccaca tcagcgcaaa ctgtaccccc
    20761 atcagctcca gccccagcca gacgatgaca cctgtccata aactgagtag cgtcttcaat
    20821 gcaagatagt gcgaaacgcc tttaagtgcg cggtgtaatc ccgcgatgtg aatctgtggg
    20881 ttattcagcg caaaacgcat tttgtaaggg acgtggcgca cttcaaacag cataaaaact
    20941 acggtcatca ccagcaaaag cacgctcgcc attgccccgg aaagcccggt cattagcgct
    21001 gtggtgaagg taaccacttt ttccgagtcc atccgctgca gcattcgctc cggcgacata
    21061 tgcaaattaa gaaaaggcaa catctcctgc aatttaaaaa gtttgcgcgt caactcctta
    21121 ttaaacttcg gcagcataga gataaattcg ttaaacgatg ccgccagtac gccgaccagc
    21181 gcagttagtg cgatcagcat caccaccacc acaatcgtaa tggcaatggg gcgttgtact
    21241 ccccgacgaa taaaccaggt gacgagcggg ttgaggacga tggcaaaaaa cagcgccagt
    21301 aacagctgca caatgatatc tgccgctgcg tgaatgcccg cgaggatcac taccagcgag
    21361 gccagcttga gcagaatgtg catgcccgtt ttatcgggtt gaggggtttc cattggggct
    21421 tccttgtgac tttttgtatt aagtgtagcg ggagtcacgc cagcaatatt ctgattctta
    21481 ctgctgattt tcacgtcagc acgtggtaaa agtgaaacac tgttgtaaaa atgtggtgat
    21541 cctcatgccc gaacccgtag ccgaacccgc gctaaacgga ttgcgcctga atttgcgcat
    21601 tgtctctata gtcatgttta acttcgccag ctacctcacc atcgggttgc cgctcgctgt
    21661 attaccgggc tatgtccatg atgtgatggg ctttagcgct ttctgggcag gattggttat
    21721 cagcctgcaa tatttcgcca ccttgctgag ccgtcctcat gccggacgtt acgccgattt
    21781 gctgggcccc aaaaagattg tcgtcttcgg tttatgcggc tgctttttga gcggtctggg
    21841 atatctgacg gcaggattaa ccgccagtct gcccgtcatc agcctgttat tactttgcct
    21901 gggacgcgtg atccttggga ttgggcaaag ttttgccgga acgggatcga ccctgtgggg
    21961 cgttggcgtg gttggctcgc tgcatatcgg gcgggtgatt tcgtggaacg gcattgtcac
    22021 ttacggggcg atggcgatgg gtgcgccgtt aggcgtcgtg ttttatcact ggggcggctt
    22081 gcaggcgtta gcgttaatca ttatgggcgt ggcgctggtg gccattttgt tggcgatccc
    22141 gcgtccgacg gtaaaagcca gtaaaggcaa accgctgccg tttcgcgcgg tgcttgggag
    22201 cgtctggctg tacggtatgg cgctggcact ggcttccgcc ggatttggcg tcatcgccac
    22261 ctttatcacg ctgttttatg acgctaaagg ttgggacggt gcggctttcg cgctgacgct
    22321 gtttagctgt gcgtttgtcg gtacgcgttt gttattccct aacggcatta accgtatcgg
    22381 tggcttaaac gtagcgatga tttgctttag cgttgagata atcggcctgc tactggttgg
    22441 cgtggcgact atgccgtgga tggcgaaaat cggcgtctta ctggcggggg ccgggttttc
    22501 gctggtgttc ccggcattgg gtgtagtggc ggtaaaagcg gttccgcagc aaaatcaggg
    22561 ggcggcgctg gcaacttaca ccgtatttat ggatttatcg cttggcgtga ctggaccact
    22621 ggctgggctg gtgatgagct gggcgggcgt accggtgatt tatctggcgg cggcgggact
    22681 ggtcgcaatc gcgttattac tgacgtggcg attaaaaaaa cggcctccgg aacacgtccc
    22741 tgaggccgcc tcatcatctt aaaacttact gaataaccag cgtattaatg atgttttctg
    22801 cggtggtctg cgctttttgc tgatcgtcag cgggcagcgt aatttgcatg gtcagcagtt
    22861 gattacccac gttacccaga ataacggaag agtacgccgt ctggcctttc gcggagataa
    22921 tactgtctaa ctgctgcatt ttgtgacctt tcagctcaat ggctttattg gttaccactt
    22981 gcagctgcgg atcgcggcta cgttgctgat cttccagacg cttcgccagc accgccagat
    23041 cttctttcgg atcatcgccc atgatgacga tgactgcttt ctgcccggtg gcgtcggacc
    23101 agacatgcat gttattggcc tgcgttccca gcttaccgct ctggtcggtc atatccgctg
    23161 gcagcgagaa acttaacttg ccatcaagca ggttgacggg attcccggta gcgttacttt
    23221 ccgcgaccga accctgcgcc gtagcgttag tgtctttatc atcacaggcc gcaagcccca
    23281 taaccagcag gccaattccg acatatttaa ccagattgcg cattgacttc ttcctttcga
    23341 taaacggcca taacggctca ttcatccatc ttatcacaac tctgataacg aacctttaac
    23401 tcgcctgcaa agcgttgatt tcggatttat ctgccagtct tttcaacagc atattgagta
    23461 atacgccata cattggcagg aagaaaacga tactgattaa cactttgaaa cagtaatcga
    23521 ccagcgcgat ttccatccag tgttcagcca taaaggcatc cgggctacgc cagaaggcaa
    23581 tgaagaaaaa ggccagcgtg tcactgacgt taccgaacag tgtggacgct gtcggtgcca
    23641 gccaccagcg gcgactttga cgcaggcggt taaaaacgtg cacgtcgagg atttgcccca
    23701 gcgcgtaggc catgaaactg gcggtggcga tacgggcgac aaacaggttg aagtgggcga
    23761 gtgcgccgaa tccctgccat gaacccatat agaacagcga cgagatgaca taggagatta
    23821 ataacgcagg gatcattacc gcgaagataa tgcgtcgggc cagcggtgcg ccaaaaatac
    23881 gcacggtcag gtcggtcgcg agaaagataa acggaaaact aaacgcgccc caggtggtat
    23941 gaaaaccaaa aatggagacc ggaagctgga ccagatagtt actggaggtg atcaccagca
    24001 gatgaaataa cgataaccag aacaacgcct tatagcgttg agtttgcgag aaaacgttca
    24061 tattgtacct ttttgattaa ccattggggt gagggaaccc aatacgtacg acacgtctgt
    24121 tatcttaaca taataacgtg cttacccttt tttcgagccg ccgcatgata ctgctttacg
    24181 ttgacaatgc aatggttaat tttcacgcaa tcgttaacct ggtttgctta cggactcaca
    24241 gggcgtaaac tagcgccgtt tttttaagtg atgagaagaa aatgaccgat ctcttttcca
    24301 gccctgacca cacactcgac gcgcttggcc tgcgctgccc ggaaccggtg atgatggtgc
    24361 gcaaaaccgt gcgcaatatg cagcctggcg aaactttact gattatcgcc gacgatccgg
    24421 cgacgacccg cgatattcct gggttttgta cctttatgga acacgaactg gttgcgaaag
    24481 agacggatgg actgccttat cgttatttga ttcgtaaagg cggttgatgg ggaaagattg
    24541 gcttcgatgc cgccttttcc cctcacccta accctctccc cagaggagcg aggggaccga
    24601 tcgcgctcaa tgttgcgatc ggtttgcctt atctcctgcg caacaatctt aacgcattcg
    24661 ctgttaccag cacggtcgca ccggtatctg ccagcaccgc cagccatagt ccggtcatcc
    24721 ctagcagcgt ggtgacgagg aagatccctt tcagccccag cgcaatagtg atgttctggc
    24781 ggatattggc gtgagtggcg cgtgccagtt caatcatttg caccaggccg cgcaggtggt
    24841 tatgggttaa tgctgcgtcg gcggtttcca gcgccacgtc tgtgccactg cccattgcaa
    24901 tcccaatggc ggcagctttc atcgctggcg cgtcgttaat accatcaccg accatcgcca
    24961 ggggcgcatg ttgattcagc ttggtcaccg ctttgacttt atcttccggc aataggcccg
    25021 ctttaaactc cagccccagc tccccggcaa ttgccgccgc tgcgcgtgga ttatcgccgg
    25081 tgaggatcac tcctttaacc cccagcgcgt tcagttcact gatggcagtt gctgcgtcgg
    25141 cgcgcagggt atcctgcaat gcaatgatac ccagcacgtc atcattacgt actaccagca
    25201 caaccgtttg cccggcgctt tccagttcat taatcaaacc agcaaatgca tcagcgggat
    25261 gtttcccggc agcgcaaatc aatacgcgct caccgttaac ctgcgcttca atgccagacc
    25321 cgaccagcgc ccgctgtgat tcggcggtgg gaatggcgag ttcagcaacc tgtgcttcgc
    25381 gtacgatggc ctgcgccagt ggatgcgtcg cgccttgctc gaccgccgcc gccagtgtca
    25441 gcagttcaga ttcactaata cccgttgccg gatgaatcgc ggtaacgcgc ggtttaccga
    25501 cggtcagcgt accggtttta tcaaacgcca cctgagtaac acgacccagc tgttccagcg
    25561 ccgctccgcc tttaatcaac gccccacgac gcgctgccgc cgccagcccg gaggtaatcg
    25621 ccgcaggcgt ggagataact aacgcacacg ggcagccaat cagcagcagc gtcagccctt
    25681 tataaatcca ctcctgccag ctggcggcaa acagcagcgg tggcaccagc gtcaccagca
    25741 gggcgacggc cataatcgct ggcgtataaa tacggctgaa acggtcgata aaccgctcaa
    25801 tgggggcgcg gcgctcttcg gcttcttcga tcagtttcag aatccggtca atggcgctgg
    25861 cacccggttc tgacagcact tccagcgtta ccagacggtc tacgctggtg gctcctgcag
    25921 gaactttatc gcccgtcgcg cgctccaccg gaatggattc tccggtcagg gcgctttcat
    25981 caaaactggc aaacggtgag agcagtttac cgtcggcagg caaacgccca cctgcggcga
    26041 cttcaatcac atcgccaggc cgcaggctgt taatcgccac ctcttcccgc tcaccgttac
    26101 gcaggcgcgt ggcggtttct ggtttcagcg ccattaacgc gctaacgccc tgacgcgcgc
    26161 ggctggcggc ccagccttcc agtcgttcac caatcaaaaa cagcagcaac accatcgcag
    26221 cttcagccgt tgcgccaata aacagtgcac caatagcggc tacgctcatt aaggtttcaa
    26281 tggcgaagta gctgccggat ttgatcagcc gtaatgcctg acgagcaatc gggtacagcc
    26341 caaccagcgt ggtcgcgata aacgccagtt gcccaaacgg atgattaaat tgctccagac
    26401 cccagctgat tgccatcatc acgattagcg taatcagcgg cagattctct ttcaggcgcg
    26461 atgcttgtgg ctcgtcggcg gcctgctcat cgcgcaggga atagcccgct ttttgcaccg
    26521 cagattcaac ctgggcacgg atgtcattgt cggcatcgac caccagtttt tcggtggcga
    26581 acagtacctg cacctgattc acacctgcaa gctggcgcac ggcattttct accttgcgcg
    26641 cacaggcggc gcagtccatg ccgctgactt tccagctata gcgggtgccg gagacgtttt
    26701 cagagagcgt tggcgagctg gaacatgcgc cgtcgcagca acagtcgttg gcgttctgta
    26761 ccgtggttag cggtttgaac gcagcaaatt gaggggcttt cttgccgtga ttgtcaggag
    26821 tcgacatggc atcctccggt taagtttttt ctcattaacc gaaggataca ctctggagtc
    26881 gactccagag tcaagtttta tcagagatac agcgagcgga cgatgaggaa atgaccggca
    26941 aagtagcagg ccgcagcaat cgcgttatct gcccggaagc ggcggcgata gtggctaccc
    27001 agccagacaa agttactgat aaacagcaat gaagcgccaa caaacgcaga gagtgccgga
    27061 gcggtcggac ggaagaacca cagttcacct gccagccaca ccatcaccag cgtcatgccg
    27121 ataaacgtgc agataggcca acgatactct tccagtcgcg tccagataat cgccagtaac
    27181 agcgcgccca gaaccagcaa caccagtggc agaggccaga agaaagagag cgtcatctga
    27241 ctggcgaaat agatggtgta cagcaggtgc gagaggaaaa acgcgccgat ggcgtacatc
    27301 agacgttgac gtggcaacag ggttagcgca tcgcccagca gtgaggcgca cagccctgcc
    27361 agcaccagat agctaatggc gtcgaacatc ggcgcttgcc aggccagtaa cagcaggaga
    27421 agaagggtta acggtttaaa cacccagcgt tgccaggttg gcccacgata cgatgcatcc
    27481 acagatagcc atgcggaaag acagacagcg ataaacgacc aaagcatctt agttccttga
    27541 attcgttatt ttttcgtaag cggaatgcct acggtcttgc ttccagttta gtggcgaggg
    27601 tggacggatg acaacaccat aactggcagg gtatgcttat ttccgcattt tccgatgagg
    27661 gataaagatg agcaaaccac ctcttttctt tattgttatc attggcttaa ttgtcgtcgc
    27721 cgcatcgttt cgttttatgc aacagcgacg ggaaaaagct gataatgata tggctccgct
    27781 ccagcaaaag ctggtggtgg tgagcaacaa gcgggaaaaa ccgattaacg atcgccgttc
    27841 gcgccagcag gaagtgactc cggcaggcac cagtatgcgc tatgaggcaa gcttcaaacc
    27901 gcaaagcgga ggaatggagc agacgtttcg cctcgacgcc cagcagtacc acgccctgac
    27961 agtgggcgat aaaggtacgc tgagctataa aggaacgcgc tttgtcagct ttgtaggcga
    28021 acaataaggt tattttttaa ctttaaattt cttctgccag accagcagtt caaacacgcc
    28081 gaaaaggaaa atccgcacct tttcggcggt ggtcatctgc gggccatctt tcggtaaagt
    28141 ggatttcaat agcgccagtt gcataccatg catcagcacg gtaaaaatca gcgccacgtt
    28201 aacgaaaata ttcagcgggc gcgggaaggg atgcaccaga ttgaggatta aaaatcccca
    28261 aacgcagagc attaacaaac gaccaatatt aatcagcatc actttctcct tgtgcttcgc
    28321 gttgatacag ccgataagcc acctgacccg ccactttttc ccgatgtaat gaccagtttg
    28381 ccggaacagt gggcagaccg ttttcgactt cgctttcgac ataaatcagg gcttcgtcag
    28441 ccagccagcc gttatcttcc agtaaattta tcgtctcttc taacaagcca cggcggaacg
    28501 gtggatcgac aaacacgata ttatgcggtg tgcctttttg cgccaggaat gacattgcgt
    28561 tgctgttcac cacgcgtgca ttgcctgctt ttagtgtcgc cagattctta attaactgct
    28621 gagaaactgc gcgatccatc tcaatcaacg tggcccccgc agcgtagcgc gataacgctt
    28681 ccagccccag cgcgccgctc ccggcgaagc aatccagaca ttgggcgtca acaatgaccg
    28741 gagccagcca gttaaacaac gtttcgcgta cgcggtcggt ggtggggcgc agacctgggc
    28801 tatcaggaac cgggagttta cggcctcgcc actgcccgcc aataatgcgg atttggccgc
    28861 tgccggaatg attcggtttt ttcatgataa attgctcaat ccgccagata acacatactt
    28921 gcaggcggtg atgtgaatta agttaagtga tagactattt catcattttt ttagctgcta
    28981 tgtacatagc gttaacgctg tgccatgaag caacagcgag gagtgtagtc gcaaatggcg
    29041 aaagaaaaaa aacgtggctt tttttcctgg ctgggctttg gtcaaaaaga gcagaccccg
    29101 gaaaaagaga cagaagttca gaatgaacaa ccggttgtag aagaaatcgt tcaggcgcaa
    29161 gagcctgtga aggcttctga acaagccgtc gaagagcagc cgcaggcgca tactgaagcc
    29221 gaggcggaaa cttttgctgc cgacgttgtg gaagtcactg aacaggttgc tgaaagtgaa
    29281 aaagcgcagc ctgaagcgga agtcgttgca cagccggaac cggtcgtaga agaaacgccg
    29341 gagccagtgg ctatcgagcg tgaagagctg ccgttgccgg aagacgtcaa cgccgaagag
    29401 gtttcgccag aagagtggca ggctgaagcg gaaaccgtag agattgtcga agcggcggaa
    29461 gaagaagcgg ctaaagaaga aatcaccgac gaagagctgg aagcacaggc gctggctgcc
    29521 gaagcggcag aagaggcggt gatggtggtt cctccggtag aagagcagcc ggtggaagaa
    29581 atcgctcagg agcaggaaaa accgaccaaa gaaggtttct tcgcgcgcct gaaacgcagc
    29641 ctgttaaaaa ccaaagaaaa tctcggttcc ggatttatca gcctgttccg cggtaaaaaa
    29701 atcgacgatg atctgtttga ggagctggaa gaacagctgt tgatcgccga tgtgggcgtg
    29761 gaaaccacac gtaaaattat caccaatctg acggaaggcg catcccgcaa gcagcttcgt
    29821 gatgccgagg cgctctatgg cctgctgaaa gaagagatgg gcgagattct ggcgaaagtc
    29881 gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt gggcgtcaac
    29941 ggtgtgggta aaaccacgac gattggtaag ctggcgcgtc agtttgagca gcagggtaaa
    30001 tcggtgatgc tggcggcggg cgatactttc cgtgcagcag cggttgaaca gcttcaggtc
    30061 tggggtcagc gcaacaatat tccggtgatt gcccagcata ctggtgcgga ttccgcctct
    30121 gttatcttcg acgccattca ggcagctaaa gcgcgtaata tcgacgtcct gattgccgat
    30181 accgccggac gtctgcagaa caaatcgcac ctgatggaag agttgaagaa aatcgtccgc
    30241 gtgatgaaga aactcgacgt tgaagcgccg catgaagtta tgctgactat cgatgccagc
    30301 accgggcaga acgcggtaag ccaggccaaa ctgttccatg aagctgttgg cttaaccggc
    30361 atcacgctaa cgaaactgga cggcacggcg aaaggcgggg taattttctc ggtggctgac
    30421 cagtttggta tccctatccg ctacattggt gtcggcgaac gtattgagga tttgcgtccg
    30481 tttaaggcgg acgactttat agaggcactt tttgcccgag aggattaaca atgattcgct
    30541 ttgaacatgt cagcaaggct tatctcggtg ggagacaggc gctgcagggc gttacgttcc
    30601 atatgcagcc gggtgagatg gcgtttctga ccggtcattc cggcgcaggg aaaagtaccc
    30661 tcctgaagct gatctgtggg attgagcggc ccagcgccgg gaaaatctgg tttagcggcc
    30721 atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag attggcatga
    30781 ttttccagga tcaccatcta ctgatggacc gtactgtcta cgataacgtg gcaatcccgc
    30841 tgattatcgc cggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg gcactggata
    30901 aagtcgggct actggacaaa gcgaagaact tccctattca gctttctggc ggtgaacaac
    30961 agcgtgttgg cattgcccgc gcggtggtga acaagcccgc ggtactgctg gcggacgaac
    31021 cgactggtaa cctggacgac gcgttgtcgg aagggattct acgtctgttt gaagagttta
    31081 accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat caacctgatc tcgcggcgtt
    31141 cctatcgcat gctcaccctg agcgatggtc acttgcatgg aggcgtgggc catgaataag
    31201 cgcgatgcaa tcaatcatat tcggcagttt ggcgggcgtc tggatcgctt ccgtaaatcg
    31261 gtcggcggct ctggcgacgg cggtcgtaac gcgccaaaac gcgcgaaatc ctcgccaaaa
    31321 ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt ccacggcgca
    31381 ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt tatcgccatc
    31441 tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca ggcggcgacg
    31501 caatattatc cgtcaccgca aatcactgtt tacttgcaaa aaacgctgga cgatgacgcc
    31561 gcagcaggcg tggtagcaca gctacaggcc gagcagggcg tggagaaagt gaactatctt
    31621 tctcgtgaag acgcactggg cgagttccgt aactggtctg gttttggtgg tgcgctggat
    31681 atgctggaag aaaacccgct tccggcagtg gcggtggtga tcccgaaact cgattttcag
    31741 gggacggaat cactgaatac gctgcgtgat cgtatcaccc agattaacgg cattgacgaa
    31801 gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt tgaccgggct ggtcgggcgc
    31861 gtttccgcga tgatcggcgt gttgatggtg gcagcagtgt tcctcgtcat cggcaacagt
    31921 gtgcgtctga gcatctttgc tcgccgtgac tccattaacg tgcagaaact gattggtgcg
    31981 acggatggtt tcatcctgcg cccgttcctg tacggaggcg cattgctggg attttctggc
    32041 gcattgctgt cattaatttt gtcagaaatt ctggtactgc gtttgttatc ggcggttgca
    32101 gaagtggcac aggttttcgg aacgaagttt gatatcaatg gtttatcgtt cgatgaatgc
    32161 ctgctattgc tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct tgccacggta
    32221 caacatttac gccactttac gcctgaataa taaaagcgtg ttatactctt tccctgcaat
    32281 gggttccgta gcagggaaag agatcccgtt gtctcttccc ggtatttcat ctctatgtca
    32341 cattttgtgc gtaatttatt cacaagcttg cattgaactt gtggataaaa tcacggtctg
    32401 ataaaacagt gaatgataac ctcgttgctc ttaagctctg gcacagttgt tgctaccact
    32461 gaagcgccag aagatatcga ttgagaggat ttgaatgact gacaaaatgc aaagtttagc
    32521 tttagcccca gttggcaacc tggattccta catccgggca gctaacgcgt ggccgatgtt
    32581 gtcggctgac gaggagcggg cgctggctga aaagctgcat taccatggcg atctggaagc
    32641 agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt catattgctc gtaattatgc
    32701 gggctatggc ctgccacagg cggatttgat tcaggaaggt aacatcggcc tgatgaaagc
    32761 agtgcgccgt ttcaacccgg aagtgggtgt gcgcctggtc tccttcgccg ttcactggat
    32821 caaagcagag atccacgaat acgttctgcg taactggcgt atcgtcaaag ttgcgaccac
    32881 caaagcgcag cgcaaactgt tcttcaacct gcgtaaaact aagcagcgtc tgggctggtt
    32941 taaccaggat gaagtcgaaa tggtggcccg tgaactgggc gtaaccagca aagacgttcg
    33001 tgagatggaa tcacgtatgg cggcacagga catgaccttt gacctgtctt ccgacgacga
    33061 ttccgacagc cagccgatgg caccggtgct ctatctgcag gataaatcat ctaactttgc
    33121 cgacggcatc gaagatgata actgggaaga gcaggcggca aaccgtctga ccgacgcgat
    33181 gcaaggtctg gacgaacgca gccaggacat catccgcgcg cgctggctgg acgaagacaa
    33241 caagtccacg ttgcaggaac tggctgaccg ttacggtgtt tccgctgaac gtgtgcgcca
    33301 actggaaaag aacgcgatga aaaaacttcg cgccgctatt gaagcgtaat ttccgctatt
    33361 aagcagagaa ccctggatga gagtccgggg tttttgtttt ttgggcctct acaataatca
    33421 attccccctc cggcaaaacg tcaatcccca cgcagattgt taataaactg tcaaaatagc
    33481 tattccaata tcataaaaat ggggtatgtt ttagcagagt attctgctaa agcacgggta
    33541 gtcatgcata aaacgaaata aagtgctgaa tcacaacacc acaacacacg taataaccag
    33601 aagaatgggg attctcagga tgaacataaa gggtaaagcg ttactggcag gatgtatcgc
    33661 gctggcattc agcaatatgg ctctggcaga agatattaaa gtcgcggtcg tgggcgcaat
    33721 gtccggtccg gttgcgcagt acggtgacca ggagtttacc ggcgcagagc aggcggttgc
    33781 ggatatcaac gctaaaggcg gcattaaagg caacaaactg caaatcgtaa aatatgacga
    33841 tgcctgtgac ccgaaacagg cggttgcggt ggcgaacaaa gtcgttaacg acggcattaa
    33901 atatgtgatt ggtcacctct gttcttcatc aacgcagcct gcgtctgaca tctacgaaga
    33961 cgaaggcatt ttaatgatca ccccagcggc aaccgcgccg gagctgaccg cccgtggcta
    34021 tcagctgatc ctgcgcacca ccggcctgga ctccgaccag gggccgacgg cggcgaaata
    34081 tattcttgag aaagtgaaac cgcagcgtat tgctatcgtt cacgacaaac agcaatacgg
    34141 cgaaggtctg gcgcgagcgg tgcaggacgg cctgaagaaa ggcaatgcta acgtggtgtt
    34201 ctttgatggt atcaccgccg gggaaaaaga tttctcaacg ctggtggccc gtctgaaaaa
    34261 agagaatatc gacttcgttt actacggcgg ttatcacccg gaaatggggc aaatcctgcg
    34321 tcaggcacgc gcggcagggc tgaaaactca gtttatgggg ccggaaggtg tggctaacgt
    34381 ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg ctggtgacca agccgaagaa
    34441 ctacgatcag gttccggcga acaaacccat tgttgacgcc atcaaagcga aaaaacagga
    34501 cccaagcggc gcattcgttt ggaccaccta cgccgcgctg caatctttgc aggcgggcct
    34561 gaatcagtct gacgatccgg ctgagatcgc caaatacctg aaagcgaact ccgtggatac
    34621 cgtgatgggt ccgctgacgt gggatgagaa aggtgatctg aaaggctttg agttcggcgt
    34681 atttgactgg cacgccaacg gcacggcgac cgatgcgaag taatcattaa tcggcaactt
    34741 tgggttgccg ccaaattgct aatatcgagt acgttgcttc atgccggatg cggcgtaaac
    34801 gccttatccg gcctacaaga tccaaagaaa tcagtaaatt gcaacacaca ttgtaggcct
    34861 gataagcgta gcgcatcagg caatttctcg ttggtcatca cttttgaaat caacttgacg
    34921 attaacgctt ctcccagccg ccctgttgcg ccgtaaaccc cagcgcctgc ataaacgccg
    34981 tcatcacacc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa acggcaggat
    35041 tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg gtgacttccc
    35101 gcacgcgcag ggaatccagt gctccctcgg tgccgcttaa ggttacccgc acggccgcta
    35161 gcaggcgctc gttaaaacgc gcggcgtaga tgcggtggtt atcgtcaacc tgtaaagagg
    35221 aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt
    35281 ctaatcgaat gatggtcagc ttcatgggta acccgtgtaa atcacaaaag tggaaccagt
    35341 gtagcgaaat aatttaatcg gaggctttct cttttttatt tcttttggca ggtgattaat
    35401 tttttaacag c
//
LOCUS       DAZFWP020000036        35200 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFWP020000036 DAZFWP020000000
VERSION     DAZFWP020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.036"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            98..649
                     /gene="maa"
                     /locus_tag="ACHK0Z_RS07985"
                     /old_locus_tag="pgaptmp_001597"
     CDS             98..649
                     /gene="maa"
                     /locus_tag="ACHK0Z_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU4501638.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            765..1235
                     /gene="ylaC"
                     /locus_tag="ACHK0Z_RS07990"
                     /old_locus_tag="pgaptmp_001598"
     CDS             765..1235
                     /gene="ylaC"
                     /locus_tag="ACHK0Z_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU4501639.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            1399..2949
                     /gene="pdeB"
                     /locus_tag="ACHK0Z_RS07995"
                     /old_locus_tag="pgaptmp_001599"
     CDS             1399..2949
                     /gene="pdeB"
                     /locus_tag="ACHK0Z_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU4501640.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2991..3344)
                     /gene="ybaA"
                     /locus_tag="ACHK0Z_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             complement(2991..3344)
                     /gene="ybaA"
                     /locus_tag="ACHK0Z_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HGU4501641.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(3464..3560)
                     /gene="ffs"
                     /locus_tag="ACHK0Z_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     ncRNA           complement(3464..3560)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK0Z_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3723..4034
                     /locus_tag="ACHK0Z_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             3723..4034
                     /locus_tag="ACHK0Z_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU4501642.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(4065..4637)
                     /gene="ybaY"
                     /locus_tag="ACHK0Z_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             complement(4065..4637)
                     /gene="ybaY"
                     /locus_tag="ACHK0Z_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU4501643.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            4855..5715
                     /gene="tesB"
                     /locus_tag="ACHK0Z_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             4855..5715
                     /gene="tesB"
                     /locus_tag="ACHK0Z_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU4501644.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(5763..7049)
                     /gene="amtB"
                     /locus_tag="ACHK0Z_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             complement(5763..7049)
                     /gene="amtB"
                     /locus_tag="ACHK0Z_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU4501645.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(7079..7417)
                     /gene="glnK"
                     /locus_tag="ACHK0Z_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             complement(7079..7417)
                     /gene="glnK"
                     /locus_tag="ACHK0Z_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU4501646.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(7598..9379)
                     /gene="mdlB"
                     /locus_tag="ACHK0Z_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             complement(7598..9379)
                     /gene="mdlB"
                     /locus_tag="ACHK0Z_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4501647.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(9372..11144)
                     /gene="mdlA"
                     /locus_tag="ACHK0Z_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             complement(9372..11144)
                     /gene="mdlA"
                     /locus_tag="ACHK0Z_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4501648.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(11174..11632)
                     /gene="decR"
                     /locus_tag="ACHK0Z_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             complement(11174..11632)
                     /gene="decR"
                     /locus_tag="ACHK0Z_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU4501649.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(11786..12604)
                     /gene="cof"
                     /locus_tag="ACHK0Z_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             complement(11786..12604)
                     /gene="cof"
                     /locus_tag="ACHK0Z_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU4501650.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            12704..14404
                     /gene="ybaE"
                     /locus_tag="ACHK0Z_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /pseudo
     CDS             12704..14404
                     /gene="ybaE"
                     /locus_tag="ACHK0Z_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            14469..15164
                     /gene="queC"
                     /locus_tag="ACHK0Z_RS08060"
                     /old_locus_tag="pgaptmp_001612"
     CDS             14469..15164
                     /gene="queC"
                     /locus_tag="ACHK0Z_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU4501651.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(15216..15614)
                     /gene="fadM"
                     /locus_tag="ACHK0Z_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             complement(15216..15614)
                     /gene="fadM"
                     /locus_tag="ACHK0Z_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU4501652.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(15708..16079)
                     /gene="ybaV"
                     /locus_tag="ACHK0Z_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             complement(15708..16079)
                     /gene="ybaV"
                     /locus_tag="ACHK0Z_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU4501653.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(16230..18101)
                     /gene="ppiD"
                     /locus_tag="ACHK0Z_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             complement(16230..18101)
                     /gene="ppiD"
                     /locus_tag="ACHK0Z_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4501654.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNEGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(18293..18583)
                     /gene="hupB"
                     /locus_tag="ACHK0Z_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             complement(18293..18583)
                     /gene="hupB"
                     /locus_tag="ACHK0Z_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU4501655.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(18774..21128)
                     /gene="lon"
                     /locus_tag="ACHK0Z_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             complement(18774..21128)
                     /gene="lon"
                     /locus_tag="ACHK0Z_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU4501656.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(21316..22590)
                     /gene="clpX"
                     /locus_tag="ACHK0Z_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     CDS             complement(21316..22590)
                     /gene="clpX"
                     /locus_tag="ACHK0Z_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU4501657.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(22716..23339)
                     /gene="clpP"
                     /locus_tag="ACHK0Z_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     CDS             complement(22716..23339)
                     /gene="clpP"
                     /locus_tag="ACHK0Z_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU4501658.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(23585..24883)
                     /gene="tig"
                     /locus_tag="ACHK0Z_RS08100"
                     /old_locus_tag="pgaptmp_001620"
     CDS             complement(23585..24883)
                     /gene="tig"
                     /locus_tag="ACHK0Z_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4501659.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(25227..25544)
                     /gene="bolA"
                     /locus_tag="ACHK0Z_RS08105"
                     /old_locus_tag="pgaptmp_001621"
     CDS             complement(25227..25544)
                     /gene="bolA"
                     /locus_tag="ACHK0Z_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU4501660.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            25849..26427
                     /gene="yajG"
                     /locus_tag="ACHK0Z_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             25849..26427
                     /gene="yajG"
                     /locus_tag="ACHK0Z_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4501661.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            26471..27946
                     /gene="ampG"
                     /locus_tag="ACHK0Z_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             26471..27946
                     /gene="ampG"
                     /locus_tag="ACHK0Z_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU4501662.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            28407..29354
                     /gene="cyoA"
                     /locus_tag="ACHK0Z_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             28407..29354
                     /gene="cyoA"
                     /locus_tag="ACHK0Z_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU4501663.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            29376..31367
                     /gene="cyoB"
                     /locus_tag="ACHK0Z_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             29376..31367
                     /gene="cyoB"
                     /locus_tag="ACHK0Z_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU4501664.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            31357..31971
                     /gene="cyoC"
                     /locus_tag="ACHK0Z_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             31357..31971
                     /gene="cyoC"
                     /locus_tag="ACHK0Z_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU4501665.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            31971..32300
                     /gene="cyoD"
                     /locus_tag="ACHK0Z_RS08135"
                     /old_locus_tag="pgaptmp_001627"
     CDS             31971..32300
                     /gene="cyoD"
                     /locus_tag="ACHK0Z_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU4501666.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            32312..33202
                     /gene="cyoE"
                     /locus_tag="ACHK0Z_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             32312..33202
                     /gene="cyoE"
                     /locus_tag="ACHK0Z_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU4501667.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            33387..33698
                     /locus_tag="ACHK0Z_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             33387..33698
                     /locus_tag="ACHK0Z_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4501668.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            33682..34224
                     /locus_tag="ACHK0Z_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             33682..34224
                     /locus_tag="ACHK0Z_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4501669.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(34280..>35200)
                     /locus_tag="ACHK0Z_RS08155"
                     /old_locus_tag="pgaptmp_001631"
     CDS             complement(34280..>35200)
                     /locus_tag="ACHK0Z_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4501670.1"
                     /translation="ILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAE
                     SGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVR
                     VDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPED
                     FLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLR
                     VTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCA
                     TVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT         8271 a         9414 c         8562 g         8953 t
ORIGIN      
        1 ctatgtgatc tttatcacac agatgtaatg ggaacgttct cttcactgac ttttcgtctt
       61 actgtgttgc cgcattttca gcaaccggag gtcagtaatg agcacagaaa aagaaaagat
      121 gattgctggt gagttgtatc gctcggcaga tgagacgtta tctcgcgatc gcctgcgcgc
      181 tcgtcagctt attcaccgat acaatcattc cctggcggaa gagcacacat tacgccagca
      241 aattctcgct gatctattcg gtcaggtgac agaggcttat attgagccaa cgtttcgctg
      301 tgactatggc tataacattt ttctcggtaa taattttttc gccaacttcg attgcgtgat
      361 gcttgatgtc tgccctattc gcatcggtga taactgtatg ttggcaccag gcgttcatat
      421 ctacacggca acacatccca tcgaccctgt agcacgtaat agcggtgctg aactggggaa
      481 acccgtcacc atcggtaata acgtctggat tggcggacgc gcggtcatta accctggtgt
      541 gaccattggt gataacgtcg tggtagcctc aggtgcagtt gtcacaaaag atgtcccgga
      601 caacgttgtc gtgggcggta atccagccag aataattaaa aaattgtaat cggtttttcg
      661 caactgttat gcaaaattgt ggtaaatctg ttacttcccc tctactattc ccacgttaaa
      721 atagggtgtt ccctggaaag ttgcagatac cacgaaggca aacgatgacc gaaatacaac
      781 gcctgctgac cgaaacgatt gagtctctga atacccgcga aaaacgcgac aacaaacccc
      841 gctttagtat cagttttatc cgtaaacatc cggggctgtt tatcggtatg tacgttgctt
      901 tttttgccac cctggcggtg atgttgcagt ccgaaacgct gtcaggctct gtctggctac
      961 tggttgtatt atttatcctg cttaatggtt tcttcttttt cgatgtctac ccacgctacc
     1021 gctatgaaga tatcgacgtg ctggatttcc gcgtttgcta taacggcgaa tggtacaaca
     1081 cgcgctttgt acctgccgcg ctggttgaag ccatcttgaa ctctccgcgt gtcgcggatg
     1141 ttcataagga acaactgcaa aaaatgatcg tccgtaaagg tgaactgtct ttttacgata
     1201 tttttaccct cgctcgcgcc gaatcaacat cttaagttag ggttacatac caggcgtaaa
     1261 gctctgcgcc tggtcaaatg acaatgatcg tttccaccca tcacttcatg aaataccagc
     1321 tctacctcct tatctcctgc cagccttttt ccacaatcag atatactttc cctacactgt
     1381 gttaataagg atatgctggt gagaacacga catctggtcg gccttatttc gggagtactg
     1441 attctttcag tattgctgcc tgtcggctta agcatctggc tggcccatca gcaggtagaa
     1501 acatcgttta ttgaagagct ggatacctat tcctcccgcg tcgctattcg ggccaataag
     1561 gtggcgacac aagggaaaga tgcgctgcaa gagctggaaa gatggcaagg cgctgcctgt
     1621 agcgaagccc atctcatgga aatgcgtcgg gtatcttaca gttatcgcta tattcaggaa
     1681 gtggtttata tcgataacaa cgttccccag tgttcgtctc tggagcatga aagtccgccc
     1741 gataccttcc ccgagccagg taaaatttcg aaagatggtt atcgtgtctg gttaacatcg
     1801 cataacgatt taggcattat ccgttacatg gtcgccatgg gaacggcaca ttatgtcgtc
     1861 atgatcgacc ccgcttcctt tattgatgtc attccctata gctcatggca aattgatgcc
     1921 gccattattg gcaatgccca taacgttgtc ataaccagca gcgatgaaat tgctcaggga
     1981 attattacca ggctacaaaa aacacccggt gagcatatcg aaaataatgg aatcatttac
     2041 gatatcctgc ccttcccgga gatgaatatt tcgatcatca cctgggcttc aacgaaaatg
     2101 ttgcagaaag gctggcatcg gcaagtcttt atttggttac cgctcgggtt ggtgattggc
     2161 ctgctggcag cgatgtttgt gctgcgtatt ttgcgccgta tccagtcacc gcatcatcgg
     2221 ctgcaggatg ctatcgaaaa tcgtgatatt tgcgtgcact atcagccgat tgtctcctta
     2281 gccaatggca aaattgtcgg tgctgaggca ctggcgcgct ggccgcagac agacggtagt
     2341 tggttgtcac cagatagttt tattccgctg gcacagcaaa cgggcctttc tgagccattg
     2401 acgctactga ttataagaag cgtctttgaa gatatgggcg actggctgcg tcagcatcca
     2461 cagcagcata tttcgatcaa tcttgaatcc accgtgctca cctcggaaaa aatcccgcaa
     2521 ttgctgcgtg aaatgatcaa tcactatcag gttaatccca gacagatcgc gcttgaactc
     2581 actgaacgcg agtttgccga tccgaaaacc agcgccccga taatttctcg ctaccgggag
     2641 gcgggccatg aaatttatct tgatgatttt ggtacgggat attcaagttt aagttattta
     2701 caggatctgg atgtcgacat tctgaagatc gataaatctt tcgttgatgc gctggaatat
     2761 aaaaatgtca cgccgcatat catcgaaatg gcaaaaacac tgaaactgaa aatggtagcg
     2821 gagggaatcg aaaccagtaa acaagaagag tggctacgcc agcatggcgt gcactacggt
     2881 cagggctggc tctacagcaa ggcattaccg aaagaagatt tcttacgctg ggccgagcaa
     2941 catttgtgaa tttttgggct gacggcacgc cgcacagccc gtcacgctac ctattcgtcg
     3001 atgattgact cgaatccgcc atagatcatt cgcttgccgt caaacggcat ggactcgccg
     3061 aactctttca tccgtgggtc cgacatcatc ttttgagtag cagcgtcgcg gacctctttt
     3121 gaagggtatt caatccagct aaagacaacc tcttcattct cttccgcttt caccgccata
     3181 cgaaaatcgg tcactttgcc atccggtaca tcgctggccc agcattcgac aatacgaagc
     3241 gcgccaaact ctttaaacaa tggcgcagcc ttagcggcca tttcccgata ggcatccttt
     3301 ttatcggcag gaacggcaac cacaaaacca tcaacatact tcatcagaca tacctccgaa
     3361 gcgtactgcg cagccagaca attcttgcga cgtcagttgt ggttaaagtt tagccatgtt
     3421 ggggtttttt gacgaaacgg tgggaggcag aaatgggtgg gggccctgcc agctacatcc
     3481 cggcacacgc gtcatctgcc ttggctgctt ccttccggac ctgacctggt aaacagagta
     3541 gcgttgcggg agaaccaaca gagcccccat tgagagcgtt gagaaccaac gcgcaggcgc
     3601 attatcactg ctgccccggc taattgcaac ccatcgcgtg ccagatgctg gtttcttgcg
     3661 caatgcgact tcactcgggc gtttttccag attatgctga aaaattacct caggaagaaa
     3721 agatggaaaa agaagattca tttccccaac gcgtctggca aatcgtcgcc gctattcccg
     3781 aaggctatgt caccacttac ggtgatgtgg cgaaactggc gggatcgccc cgcgccgcgc
     3841 gccaggtggg cggtgtgtta aagcgtctcc ctgaaggcag caccttaccc tggcaccggg
     3901 tggttaatcg ccacggcaca atttcgctaa ccggaccgga tttacagcgt cagcgacagg
     3961 cattactggc agaaggtgtg atggtatcgg gaagcgggca aatcgacttg cagcgttatc
     4021 gctggaacta ctaaccctct ccgggcggag agggttaacg tcgcttaata ttgcgtagga
     4081 gctggaactg ccgaagacgg attcacctga gttggtgaag tcgaaggtac ggtagtcgtt
     4141 gcaccaccgc tggcctgaac cggcacggcg gtttgctgca ccggcaccaa tgtcaggtcg
     4201 gctttagttc cgcccttgtt gatcaccggc tgaacggtat cggtaataaa taccagtttg
     4261 tcattcacgg taatcgccgc actcaacaga atacgcgcgt tcggctgaac atctgccggg
     4321 ttaaatggca gaacaaagct gaatggtgac tgtttacctt cagtacgcac cgctttctgc
     4381 gccagcactt ttgacggtgc atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca
     4441 gcatcaggcg gcagtgcgac tttctgacgg atccagacgg taccggagac attcggttgc
     4501 tggatagcag gttgttgtgt tgctgaaata gacgtatttg cagccggggc tggcgtctga
     4561 atatccgcgc ttttatctgc gcaagccgcc aacgcaatcg caaccgctaa accactggcc
     4621 atgtgcacga gtttcattca tttctcctta ttatcaatgc accagcgggc taactttcct
     4681 cgccggaaga gtggttaaca aaatagtaac gtcaacaagt gtggcacaca tcacgcattt
     4741 ctgcctgtaa ttagcccgta attcagacca ttgcacccat cggaccagta gcaaatttgc
     4801 gttatactca actcactttg gcttgctgcg gcagctttgt tactggagag ttatatgagt
     4861 caggcgctaa aaaatttact gacattgtta aatctggaaa aaattgagga aggactcttt
     4921 cgcggccaga gtgaagattt aggtttacgc caggtgtttg gcggccaggt cgtgggtcag
     4981 gccttgtatg ctgcaaaaga gaccgtccct gaagagcggc tggtacattc gtttcacagc
     5041 tactttcttc gccctggcga tagtaagaag ccgattattt atgatgtcga aacgctgcgt
     5101 gacggtaaca gcttcagcgc ccgccgggtt gctgctattc aaaacggcaa accgattttt
     5161 tatatgacgg cctctttcca ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg
     5221 tccgcgccag cgcctgatgg cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac
     5281 ctgctgccgc cagtgcttaa agataaattc atctgcgatc gtccgctgga agtccgtccg
     5341 gtggagtttc ataacccact gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc
     5401 cgcgcaaatg gtagcgtgcc ggatgacctg cgcgttcatc agtatctgct cggttacgct
     5461 tctgatctta acttcctgcc ggtagctcta cagccgcacg gcatcggttt tctcgaaccg
     5521 gggattcaga ttgccaccat tgaccattcc atgtggttcc atcgcccgtt taatttgaat
     5581 gaatggctgc tgtatagcgt ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc
     5641 ggtgagtttt atacccagga cggcgtgctg gttgcctcga ccgttcagga aggggtgatg
     5701 cgtaatcaca attaaaaaaa tagccggagg taaaaccgtc cggctatttt ttgcagtgct
     5761 tgttacgcgt tataggcatt ctcgccgtga ctgttgacat ccagcccttc tcgctcctgc
     5821 tcttccggta cacgcagacc aaccgtcaga tccgccagtt tgtagccaat aaatgccacc
     5881 acaccggacc agacgatcgt aatggcgatg ctttccagct gtaccagcaa ctgatggccc
     5941 atcgtcacac cttcagcgaa gcccacgcca cccagcgaac tggcggcaaa aatcccagtc
     6001 atgatacagc cgacaatgcc acaaacgccg tgcacaccga agacatcgca gggatcatcc
     6061 acccgcagca agcgtttgag catagtaacg ccccacaagc ccgccagacc agctaccacg
     6121 ccgataatca acgcgccgcc aaccccaatg tagccacacg ctggcgtcac gccgaccaga
     6181 ccggcaatcg cgccagaaca cgctcccagc agagaaggct taccacgcag cgcccattca
     6241 ccgaagatcc agccaagaat tgccgccgcc gttgcgacca cagtattcac aaatgccagt
     6301 gccgcgattt cattcgccgt gcccgctgac ccggcgttaa agccaaacca accaatatag
     6361 agaatggcag taccggtgaa gaccattggc aggttgtgcg gtttaaacgc ctctttgccg
     6421 aagcccacgc gtttgcctat cagatacgcg cccaccagac cggcgattgc ggcgttaata
     6481 tgcaccaccg tgccacccgc gaaatccagc gcaccgtgag aagccagcaa accaccgccc
     6541 cacaccatat gcgcaatcgg aatgtaagag agcgtcagcc ataccaccac gaaaatcaac
     6601 acagctgaga agcggattcg ttccgccagc gccccaacga tcaagccgac ggtaatgcag
     6661 gcaaacgagc cctgaaacgc cacgtggata tactgataaa tgctgcccat caccgccgtc
     6721 agttcgatgt tttttagcat caaccagtta atgttgccga agaagttgtt gccctcacca
     6781 aacgccagcg agtaaccgta aaccacccag agaatacaga ccagtgcaaa cgtcaccgtc
     6841 acctgcgtca gcatcgacag cacgtttttg ccgcgaatca acccaccgta aaacagggca
     6901 atccccggaa tagtcataaa cagcaccagc gcagtacaaa tcatcataaa cgcattgtcg
     6961 gctttatcgg ccaccgcagg tgcagccatt accagtcccg gcagcatcgc cagtgaagca
     7021 agcccagttt ttatcgtcgc tatcttcatt tttcgttcct gttgctgtgt gccagagatt
     7081 acagcgccgc ttcgtcggct tcgccggtac gaatacgaat gacgcgttgc aattcagcga
     7141 cgaagatttt gccgtcgcca atttttccgg tgtaagccgc cttactgacg atatcgatca
     7201 cttcatcgag ttggtcatca gcaatcgcca catcaatttt tacttttggc aggaagttga
     7261 cgctgtattc cgccccccgg tacagctcgg catgcccttt ctgacgcccg aaacctttca
     7321 cttcggtgac ggtcaggccc tgaataccaa tggaagataa cgcttcacga acgtcttcca
     7381 gcttgaatgg tttgattatc acggtcacca gcttcataga tcccctccgg tcagaattcg
     7441 gtaatggttc tgctacacga agaaggtatt gcaagcggtg tgccagaaat gaaaaacgag
     7501 aaagagagca ggaagttaag aagattgaaa atgaaaacgc actatgacag tgcacagtgc
     7561 gttacatttt tgcaccaacg atgaaatatt gcgctattca ggcgctcaat gattcttctt
     7621 cacgcacgct ggctgccagc tcttcgcccg caagttgcag ttgatacatc tgccagtagc
     7681 gtccctgggc cgccagcagt tgctggtgag tgccctgctc cacggcttgc ccacgatgaa
     7741 gcaccagaat ggtgtcggca tcaacaatgg tcgataagcg gtgagcaatc accaccagcg
     7801 tggtatgttc acgcacagcc gccagagcat gttgaatcgc ctgttcagta ccggagtcaa
     7861 tgctggcggt tgcctcatca aggatcagga tttgcggcgt ctccaccaac acgcgtgcca
     7921 gtgccagcag ttgcttttgc ccgacggaga gattattccc ctgctcgccc agcggcgtat
     7981 aaataccatc actcatgctg cgcgccagct ccgccagttg cacggtttcc agcgcctgcc
     8041 agacgcgttc ttcggagata tcacgcccca gcgtcacgtt ggcgaggaag gtatccgcca
     8101 gcaccaccgg atcttgctgc accattgcca cgccctggcg cagcgcgctg tgacttagcg
     8161 aacttaatgg acgaccatca aggcgaatct caccttccgt tagcgggtaa tagcccatca
     8221 ataaactggc gagggtgctt ttgccactgc cggtatgccc gaccagtgcc acaaagttac
     8281 gcgaaggcac agagagatta atgttcttta gcaccagatt gtcatcgcga taagcaaatg
     8341 acacgttatc gacttcgatg gtgccactct gtaacgggcg atcatcattg ccatattgct
     8401 ggcgcggtcc gtccatcagt tcaaacacgc gctcaccagc aacaacagcc tgttgcagca
     8461 tcgcctgttg cgtggtcagt tcgattaatg gttcgttaag tcgcccaaga tagctgataa
     8521 acgcatacag cacgcccact tcaatggtgc cgctagcgga gaagccaaac agcatcaaca
     8581 agccacaaag aatgagcgat gaaaacagac tcagcagcgg acgcagcaga aaaccgtcga
     8641 ggcgcagggt ttgcatcctg gccatatagt gtgaacgact ggcctccccc atacgttcgc
     8701 caaatcgcgc ctgctgacgg aactgctgga taacgctcat gccattgatg atttcgttaa
     8761 agccgtcgtt gatatccgct agataggcgc gcacacgacg gacaatcggc gtgctgtaac
     8821 gctggtatat caccattacc accagcacca ccgggaaaat cattatcgcc accagcgcca
     8881 ttcgccagtc gaggctgaac atcgccacca gcatcgcgcc caccagtgcg gcgctacgca
     8941 ggacagttgc cactacggta acgtagagat cgcggatcac ttcagtgtca ttagtgacgc
     9001 gggaaatcac ctgcccgacg ggttgggtat caaactcgct taatggctgg cgcaatgcgg
     9061 catccatcac gtcggtacgc aactgttgca ctacgcctac tgccgcccga ttaaacagca
     9121 gcgactgcgc gtaatgtagc ccggcggcaa acagttgtag cccaacatac gccgcagcca
     9181 gccctgcaac cattttcaac ggcaggttat ttttcgctac catattgtcg ataaaatagc
     9241 tgataagcag cggcccactg acttctgccg ctgccgcaac ccacatcatc aggaccgcaa
     9301 tccccagcgg tttacgccac ggcgaaccgt acgctaacag acgcttgaga gtcggccaca
     9361 gttggctaaa actacgcatc gacggcctcc tcgcgaattt ccggagcgtc gtcgagcgcc
     9421 gcctccagtt gttgatagcg atacatatcg cgataccagc cgctttgttg cgccagctca
     9481 tcatgattgc cacgctgggc gatatgtccg tgctgcatca caataatttc actggcttcc
     9541 gtcagtgccg aaaggcgatg ggcactgatg attaccgttc ttccctgtcc ccactgacgc
     9601 aggttatgca ggatctggtg ctctgtgcgt ccgtccaccg ccgaaagcgc atcatcaagg
     9661 atgaggattt ccgcgttgac taataacgct cgagcaatgg agatacgctg tttttgccca
     9721 ccggaaagca tcacgccgcg ctcgcccact tctgtatcgt aaccttgcgg tagacgcaaa
     9781 atatcgtcat gtacgctggc taaccgcgcg acatgctcaa tctcttgctg ggtggcattc
     9841 gggcaaccca acgcgatgtt gttcgctacg gtgtcggaaa aaaggaacgg tgtctggcta
     9901 actaccgcca ggcggctacg ccagctatcg agttgtaact tcgtcagagg aatatcatga
     9961 aagcgaatat ccccctcgct gacgtcgaaa tgacgctgaa tgagcgacaa cagggtactt
    10021 ttgccggaac cagtcggccc gcagataccc agcatctgac cgggtttcag ggcgaaattg
    10081 acgttttcca gcgcaggatg gtcagtctgc ggatacgtga actggtgaat atttacatca
    10141 agttcgccac gcccttccgg cacgggttcg ctaccatcgt tcaccaccgg cgcttccgcc
    10201 agcatcgcac gaatacggct gtacgcagca ctaccacgtt ccacaatgtt aaacatccat
    10261 gccagcgcca gcattggcca aatcatcaga cctaaataca tcataaaact ggtgagctgg
    10321 cccagcgtta aactgccctg cgccaccatc caactaccgc cgccaatcgc cagcaagttc
    10381 gccataccaa tcgcgatata gatggttggg tcgaaacgag catcaatacg cgccacccgc
    10441 atgttttttt tgccggtatc ttcggcatcc gcggcaaata acgccgactg gcgatcttcc
    10501 agaccaaagg ctttgatcat gcggatactg gtgaggcttt cctgggtgcg gtcattaaga
    10561 ctagaaaacg ccgcctgtgc cagcttaaag cgttcatgca aggcatcgcc gttgcgcttg
    10621 atcatgatcg ccatcactgg catcggcaac agggcaaata aggtcaactg ccagctaatt
    10681 tgcgtagaca tcataatcaa cacagcgcag cccatcacca gtgaatccac cagcgtcagc
    10741 accccttctc cggcggcaaa cacgacgcga tcgacgtcat tggtcgcacg agccatgaga
    10801 tcacccgtgc gatgacgcag gtaaaactca ggatgctgcc ggcttagctg acggtaataa
    10861 tcttcacgca gttcaacagc cagttgataa gacgcaccaa acagcaatac ccgccagacg
    10921 taacgcagga gataaaccac aacggcaatc agcaccatgg tggcgatcca catcaggatc
    10981 tgcccggtag taaagtgttg ttctgtcacg ccatcgacaa caataccaac cacttttggc
    11041 ggaaccagtt gcagcatcgc gataatgaca agcaaggcga cagccccgag atagcgacgc
    11101 cattcccgac ggaaatacca gcttaattga gcaaataatc gcacgcgttg tgtcctgacc
    11161 tgattctgga tatttattcg atgggtaaag aagtggtgta tttaatctgt tccatcgcga
    11221 agctggaagt gacgtccgac agccccggca cgctgtttac cagacgctta taaaactcgt
    11281 cgtagcgttt catgtcggca acctggacgc gcatcagata atcgtattca ccagccatgc
    11341 gccagaaccc cagcacttct ggcatttcgg taaccaccgt gacaaagcgg caataccatt
    11401 cgctgctgtg atgttgcgtt tttatcagca caaaggcggt caggccgagg cctatttttt
    11461 ccggatccag cagggcgact ttgccgataa ggataccgtc gtcctccagc cgtttcaggc
    11521 gcttccagca aggggtggtt gtcagattaa cggcttcagc cagtgcctgc aaagagaggg
    11581 tgcaatcctg ctgcagtaag gccagcagct tacggtcaat tttatctaac atagcccttc
    11641 cacggagaat ttttttctct tttaattcta ttttaaaggt gaaatcgcga caatttattc
    11701 tgtgcggttt aaacaccacg gcacaaaatg acaaattcaa cgcaaaaaaa aaccgggcaa
    11761 ttgcccggtg ctggaaggga tctcgttatt cgggggaata aggtagatgt ggatagtcca
    11821 gccagtgcgt caaatagtga gagacagcct gatttcggca atgtccaatc accggtaaat
    11881 gcgggagctc cgcgcgcagt tgcggcatcg cattgcccat aataaacccg ctaccgacgc
    11941 tgcctaacat ttcgcgatcg ttcatcgcat caccaaaggc catgcaatcg cgcaacgata
    12001 aacctaaatg ttgggtcagc accgtcaatg cagcgccttt attgcagccc accggcagca
    12061 cttcgaggca atccgtggcg gaaaaacaca aatgtgcacg ctcgcctaat gcttcgtata
    12121 gctggatctg caagcgtgta agatcgtcgt gatcgccaca gaagcagatc ttggtgacgc
    12181 tgccgagtgg catttttttg acatcgatta tctgataacg aaaaccgcta tagacaaatg
    12241 cctgcaacaa cgcagggatc tctttcccgg taaaccaacc gtcgtcattg aagatatgca
    12301 tgctggctcg ggtatcccat tgctgataca gcaccagctc cgcgacatcc gcaggtaaat
    12361 catcacgatg taaaagttca ccttccagag aatgcacgcg cgttccgttg ccggtaatca
    12421 aatatgcatc cagcgatagc gccccgagaa tatgctgcat ctccagcgca tgacgccccg
    12481 tggcaaaagt gagggtaatg tcgcgttcac gcagtcgcgc caaagtagag agggttttct
    12541 cacctaaatg atggtcaggc atcaataaag tgccatccat atcaaatgct gccagacgag
    12601 ccatttcttt ctccactctg tgacacggtt aatggtgatt gagtatcacc tgatatatac
    12661 ggaagtaata gtgaatagtt aaataatatt gttccgggtt tatatgcgat tgctcaaccg
    12721 tcttaaccag tatcaacgtc tgtggcaacc ttcagccgga aagccgcaaa ccgtcaccgt
    12781 cagcgaactg gccgaacgct gtttttgcag cgaacgccat gttcgtacgc tgttgcgtca
    12841 ggcacaggag gcgggatggc tggagtggca ggcgcagtca ggacgcggaa agcgcggaca
    12901 attacgcttt ctggtcacgc cggaatcgct acgcaatgcg atgatggaac aggcactgga
    12961 aaccggaaag cagcaagatg tgctggagct ggcgcaactg gccccaggtg agctgcgcac
    13021 tctgttacag ccgtttatgg gcggacaatg gcaaaacgat acacccacgt tgcgtattcc
    13081 ctactatcgc ccgctcgaac cgctacaacc aggctttttg cccggccgtg ccgagcagca
    13141 tctcgccggg cagatatttt ccggcctgac ccgcttcgat aataatactc agcgcccgat
    13201 tggcgattta gcgcatcact gagaaacctc tactgacggg ttacgctggg acttttatct
    13261 tcgttcaacc ctacactggc ataacggcga tgcagtaaaa gcctcacact tacaccagcg
    13321 attattgatg ctgttacaac tgccagcact ggatcaatta tttattagcg tgaagcgtat
    13381 tgaagtcacc catccgcagt gtctgacctt ctttttacat cgccccgatt actggcttgc
    13441 gcaccggctg gcgagctatt gcagccatct ggcgcatccg caattcccac tgatcggcac
    13501 tggtcctttt cgcttaacac aattcacagc ggagctggtg cgcctggaga gccatgatta
    13561 ttaccatttg cgtcatccgc tgcttaaagc ggttgagtac tggataactc cgccgctttt
    13621 cgaaaaagat ttgggaacca gttgtcggca tcccgtgcaa atcaccatcg gcaaaccgga
    13681 ggagctgcaa cgggtcagcc aggtcagtag tggcatcagt ttaggttttt gctatttaac
    13741 gttgcgcaaa agtccccgac tctccctctg gcaggcgcga aaagtgatct ccattattca
    13801 tcaatccggt ttattacaaa cgttagaagt cggagaaaac ctgatcaccg ccagtcatgc
    13861 attactgcca ggctggacta ttccgcactg gcaagtaccg gatgaagtca aactaccgaa
    13921 aaccttgacg ctggtttatc acctaccgat agaacttcat accatggcag aacgcctaca
    13981 ggcgacactg gcagcggaag gctgtgaact cacaattatt tttcataacg caaaaaactg
    14041 ggacgacacg accctactgg cacacgcaga cctcatgatg ggcgacagat taattggcga
    14101 agcaccggaa tatactctgg agcaatggct acgttgcgat ccactatggc cacatgtttt
    14161 cgacgctcca gcatatgcac atctgcaatc gacgctggac gcggtgcaag taatgcctga
    14221 tgaggaaaac cgatttaatg ccctgaaagc ggtttttagc cagttaatgg cagacgcgac
    14281 gctgacgccg ctgttcaact atcactatcg cattagtgcc cctcccggcg tgaacggtgt
    14341 gcgactgaca ccgcgcggct ggtttgaatt taccgaagcc tggcttcccg cgccgtcgca
    14401 atgaatagct ggtccgggtt aatcgcaggc gttaccataa cgcttttatt gttcaagcag
    14461 gattatctat gaaacgtgct gtcgttgtgt tcagtggagg ccaggattcc accacctgtc
    14521 tggtgcaggc attacaacaa tatgatgaag tccattgcgt gacgttcgat tacggtcagc
    14581 gacatcgcgc agaaatcgac gtggcacgcg aactggcgct gaaactggga gcacgcgcgc
    14641 ataaggttct tgatgtaacg ctgctcaacg agctggcggt cagcagcctg acacgtgaca
    14701 gcattccggt gcctgattat gaacctgaag ccgatggcat cccgaatacg tttgtccctg
    14761 ggcgtaatat tttgttcctg acgctggcgg caatatatgc gtatcaggta aaagcagaag
    14821 ccgtaattac cggcgtctgt gaaacggatt tctccggcta cccggattgc cgcgatgagt
    14881 ttgtgaaagc actaaaccat gccgtcagtt tgggcatggc gaaagatatt cgttttgaaa
    14941 cgccgctgat gtggattgat aaagcggaaa cctgggcgct ggcagattat tacggcaaac
    15001 tggatttagt ccgtaacgaa acgttgacct gctataacgg cattaaaggc gacggttgcg
    15061 gtcattgtgc ggcatgtaat ttacgcgcca acggtttgaa tcattatctg gccgataaac
    15121 cgacggtgat ggcagcgatg aagcagaaaa ccgggttgag gtaattattc cgggtgtcgc
    15181 cggatgcggc ttgagcatcc ggcaccacaa aacgtttact taaccatctg ctccagcttt
    15241 tcgcgcaatt ccccttccag agctaatgct ttctgcgttt taagatcaat acaaacaaac
    15301 gtaataagcg catccgctac cacctgccct tccggctcca gtgtaatgac ctggcttaag
    15361 atgccgcttt taccgtttaa ttgctgcaac tgactggtaa tagttaacag gtcacttaat
    15421 accgctgggc gacgatagtt aatattgata ttgaccacga cgaaggcgat gttatgggcc
    15481 gtcatccact gaaaactgtc gctattttcc aacccatccc agcgggcttc ttcgagaaat
    15541 tcaaggtagc gggcgttgtt gacgtgctgg taaacgtcga gatgatatcc acgaactttg
    15601 atttgtgttt gcatagcgca ataaccttac gttgttgtta taagtacaga ggtcataact
    15661 ggtatgacct cttcagtctg gcaaaaaatt gccactatgc aaattaatta cagggttaat
    15721 accgccagat tacgttccac cagcgaattg cccatccccg gcacctgctt tagatcctcc
    15781 accgttttaa acggaccgta ctcttcgcga taactgacaa tcgcctgcgc tttcttcagg
    15841 ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg cattattaat gctgacccgg
    15901 gtgccttctt cgtcactggc tttcgccggt actgctgctt tactttgtgc tgcaggagct
    15961 tccgctttgg tttctaccgt tgtcggtttc gccacagaag ctgccgccag cgcgctatga
    16021 gacattccgg cacaggccag ggacagggta atgagcagtg ctttaattcc gtgtttcata
    16081 ctgttttctc cttgtttgtt aacagtggaa tcaccttaac ggcagtgaaa acagtaaaca
    16141 aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac cttttcttgt tacagctgca
    16201 acgcattttt ttgcgaggcg gcttcaggat tattgctgtt ccagcgcatc gccaattttg
    16261 attttcgcct ctttacgcag gttactcatc agagcttcaa agacgatttg tgcgttgttc
    16321 tgggtgatac cctgcaccat cgcttttttc tgatcttccg gcattgaacc ttgtttcact
    16381 tcatccagcg ccagcagaac cacattacct tgcatatcgg tcgccatacc gtagctcggt
    16441 ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc taatcgggtc acgaccggaa
    16501 cggcttaaga ttttcggctc gccaaatttc agaccggcag cctgcatagc ttccgcacct
    16561 ttgccggctt tcaaatcaac cagcagtttc tcagcatcca ctttcgcctg ttgttcagct
    16621 ttgttgtgct gaaccagtgc cttaacttgt tcctgaacat ctgccaacgg tttcaccgct
    16681 tccggtttgt gctcgctgat gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg
    16741 tcagagttga tgcccggcgc gccgttttca cctaccagac cgccgttaaa gatagcgtcg
    16801 gcaaccggct tgaagttcaa ctcttccggc aggttatctt tgctaaacca acccgtctga
    16861 gtggctttaa cgccggcagc ttgctctgca ccggccagag actcggtgtc gttgcttgcc
    16921 gcatcgctca ctttctgctg cagcgcatag tacgcatcga gggctttttc gtgtttcact
    16981 ttcgccgcaa tgtcgtcacg tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg
    17041 tccagacgta caatcaggaa accgaccgaa gatttgatga caccagacag ttgccctttt
    17101 tctttcagac cagcattttt cagttcgtcg gggatagtgg catcttctaa ccaacccata
    17161 tcgccgccgt tacgggcaga gataatatcg gcagattttt ctttggctaa tgcagcaaaa
    17221 tcaccgcctt cattcagctc atcaagtacc gctttcgctt catcttcagt tttggtctgg
    17281 atgatgctgt agcgggtacg ctgcggctgg gtgaattgat cctgatgctg atcgtagtag
    17341 ctctggatat ccgcatcgct aaccggttgc tgcatcgttg cggcatccag cttgatgtaa
    17401 ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca
    17461 atctcctgtt cggtcacagg ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca
    17521 cgcaccacgc gttgttgcgc gaccagtgcc gccagctcgt cggtttcacc tttcagcata
    17581 aaatcggtac cggcaacacc gttaatcagc tgttgggtag tgagctggtt acgcagcgcc
    17641 tgggcgtact gatcggcggt catccccatc tggttgagga taccgttata gcggctgtta
    17701 tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca
    17761 tcactgatac ccagtttcag ctcacgagcg tactgatcca acagcgcctc gtcgatcaga
    17821 cgattcagca cctgttgacg cagggttttc atatagcctt cgttcgctgc cagctcggag
    17881 tattgatcgc ccagctgttg ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac
    17941 tgcccacggc tgatttcctg gtcattcact tttgcggcgt agttattgcc tccgccaatc
    18001 aggtaaccac tcacgccggt caatatgaac gacacgataa tgataccgaa aataatcttg
    18061 agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca tggtgtaaca acactccgct
    18121 gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga
    18181 aacgggggca attgtcagcc cacaagacgc agaaagtcac cgaatacaaa taaaaaaggc
    18241 acatcagtag atgcgccctt gaacttcgtc acatccccac cggggacaac gcttagttta
    18301 ccgcgtcttt cagtgcttta cctgcacgga agctcggtac tttagcagca gcgatggtga
    18361 tctctttacc ggtctgcggg ttgcggccag tacgggcagc acgctcttta acggcaaaag
    18421 taccaaaacc taccagtgct acatcatccc cttctttcag agattcagtt acggaagcaa
    18481 taatagcatc taacgcacgg ccagccgcag ctttagagat atcagcccct gcagcaatct
    18541 tgtcgatcaa ttgagattta ttcactcttc tcttcctctt tataatttat atcgcacttg
    18601 aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc agccatcccc ttacaacacc
    18661 ccttaataag ggcaagcccg atccgccatc taacttagcg agacaaaaaa aggctggcaa
    18721 gcccgaatta gcctgccagc cctgttttta ttagtgcatt ttgcgcgagg tcactatttt
    18781 gcagtcacaa cctgcatacc agacggttca ttttgtagcg ccagagtcag aacttcctca
    18841 atgcgcttca caggatgaat gtccagatcg gcaattacgt tgtcaggaat ctcttccaga
    18901 tcgcgtttat tttcgaacgg aattagcact gttttaatcc cgccgcgatg cgctgccagg
    18961 agtttttctt tcaaaccacc gatcggcagt acctgaccac gcagagtgat ctcaccggtc
    19021 attgccacat cggcacgaac cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata
    19081 gcaataccgg cactcggacc atctttcggc gtcgcacctt ccggaacgtg gacgtggatg
    19141 tcacgttttt cgtaaaaatc agggttgatc cccagttttt ccgcacgcgc acgaaccacc
    19201 gttaacgccg cctgaataga ctcctgcatc acttcgccga gcgaaccggt ataggtcagt
    19261 ttgcctttac ccggaacaca tgcggtttca atggtcagca agtcaccgcc cacttccgtc
    19321 cacgccagac cggttacctg accgacacgg ttttcgttat cagcgcgacc atagtcgaaa
    19381 cgctgaacac cgaggtagtc atgcaggtta tcgccgttaa tttcgatatg ttttaatgac
    19441 ttatcgagca gtaactgctt aaccgctttg cggcacagtt tggagatttc acgctccaga
    19501 ccacgcacgc ccgcctcacg ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg
    19561 acggtcagct cacctttttt cagtgcatta cgttcaatct gcttcggcag caggtgacgt
    19621 ttggcgatgt tcagtttttc atcttcggta taaccggaga ggcgaatcac ttccatacga
    19681 tccagcagcg gtgccggaat gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg
    19741 agatcgtaat ccacttccag gtagtggtcg ctgaacgcta cgttctgctc tggatccagc
    19801 acttcaagca gtgcagaggc cggatcgcct cgcatgtcag aagacatttt gtcgatctca
    19861 tcgagcagga acagcgggtt tttcacgccc actttcgcca ttttctggat cagtttaccc
    19921 ggcatagaac cgatgtaagt acggcggtga ccacggattt ccgcttcatc acgcacgccg
    19981 cccagcgcca tacggacata tttacgcccg gtggctttgg caatggactg accaagagag
    20041 gttttaccta cccccggcgg ccctaccagg cagaggatcg gtcccttgat tttgttgaca
    20101 cggctttgaa ccgcaaggta ctcaaggatg cgatctttca cgcgctccag accataatgg
    20161 tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc
    20221 cacggtacct gtaccatcca gtcgatataa ccacgcacta cggtcgcttc tgccgacatc
    20281 ggagacatca ttttcagctt ctgcaactct gcttccgctt tctcttttgc ctctttcggc
    20341 atcttcgccg cgtcgatttt gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt
    20401 tcaccgagtt ctttctgaat agctttcatt tgctcgttca gatagtactc acgctgggat
    20461 ttctccatct gctttttaac gcggttgcga atgcgtttct caacctgcag cagatcgatt
    20521 tccgattcca tcattgccat cagatattcc agacgttcgt taacgtcgga catctccaga
    20581 acggactgtt tgtcagccag tttcagcggc atatgtgcag caatggtatc cgccagacgc
    20641 gccggatcgt cgatgctatt cagcgacgtc agcacttctg gtgggatttt tttgttcagc
    20701 ttgatgtagc cttcgaactg gctgattgca gtacgcacca gcacttcctg ttcccgctca
    20761 tcaatggtcg gcgactccag atactccgcc ttcgcagaaa agtgttcgcc attgtcagag
    20821 agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc
    20881 agtttcagca tctgcaatat agaggccacg gtcccgacgg tgaaaagatc gtttacaccc
    20941 ggctcatccg ttgaagcttc tttctgcgcg accagcataa tttttttatc atggtccatc
    21001 gccgcttcca gacaacggat agatttttcc cgcccgacaa ataaggggat gaccatgtgc
    21061 ggataaacca ccacatcgcg cagcggcaat acggggattt caatgcgttc agaacgctca
    21121 ggattcatag agctctctct tagtttaatt tccgccaggt aatcagatga cacgactgtg
    21181 cttcacgcca tctattaaca tgtacgtcag tatatgggga tgtttccccc acattcaacg
    21241 ccgagaatag aggaaaaatt aaaggggaga taaaatcccc cctttttggt taactaattg
    21301 tatgggaatg gttaattatt caccagatgc ctgttgcgct tccggcttgc cataaatcag
    21361 caacggtttg ctttgaccat caattaccga ctcgtcgata accacttttt cgacatcttc
    21421 catggacggc agatcgtaca tggtatcgag cagtgcggct tctacgatgg aacgcaggcc
    21481 acgggcaccg gttttacgcg ccatcgcttt cttagcgata gcatccagcg cctcgtcacg
    21541 gaattccaga tccacgcctt ccagattaaa cagcgcctga tactgcttgg tcagggcgtt
    21601 tttcggctct ttgaggatct gaatcagagc ttcttcgctc agttcattca acgttgcgac
    21661 aaccggcaga cgaccaatga actcagggat cagaccaaac ttgatcagat cttccggttc
    21721 aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc
    21781 aaaaccaatg ccggagccgg tttctacacg atgggaaatc actttatcca gaccggcaaa
    21841 cgcaccgcca caaataaaca ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg
    21901 atgtttacgc ccaccttgcg gtggaacagc agctaccgta ccttcgatca gtttcaacag
    21961 tgcctgctgt acgccttcac cggaaacgtc acgggtaata gacgggttgt ctgacttacg
    22021 agaaatcttg tcgatttcat cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta
    22081 atcgcacttt tgcaacagct tctgaatgat gttttcaacg tcttcgccca cataaccggc
    22141 ttcggtcagc gtggttgcgt cggccatggt gaacgggacg tccagcaggc gcgccagcgt
    22201 ttcagccagc agcgttttac cggaaccggt cggaccgatc agcagaatgt tacttttgcc
    22261 caactcgaca ccattgctgg tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc
    22321 gaccgccagc acttttttcg cctgttcctg gccgataacg taatcgtcca ggtggttgcg
    22381 aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc
    22441 ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg cagatataca cggatggacc
    22501 ggcaatcagc ttgcgcactt catgctggct tttgccgcaa aaagagcaat acagcaattt
    22561 gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc
    22621 cgcacacgac gacgcaaatg ccattctcag gcgcaagccg cttacagcgt tgtgccgccc
    22681 tggataagta tagcggcaca gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa
    22741 tcgaccagac cgtattccac cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca
    22801 cgttcaatct gttctaatga ttgacccgta tgaagcgcca taagttcatt catgcgccct
    22861 ttaactttca gaatttcacg ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg
    22921 cccaacggtt ggtgaatcat cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc
    22981 cctgcggtca gcaagaaagc gcccatcgag gccgcctggc ccatacagat ggtgctgaca
    23041 tcaggcttga taaactgcat ggtgtcatag atagacatcc cggcagtgat caccccgcct
    23101 ggggagttaa tgtacagata gatatctttt tctgggtttt ccgcttccag gaacagcatc
    23161 tgcgccacaa tcaggttagc catgtggtct tcaacctggc cagtcagaaa aatgacgcgt
    23221 tccttaagta gacgagaata gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg
    23281 accatcggca ccagcgccat atggggtgca aagttatctc gttcgccgct gtatgacatt
    23341 tccgtctcct ggataaaatt gaaaaaacct gctgtaccga ttgtaacctt atggacggat
    23401 tatcagctag tccctgtgtt atgggtacgg catcaccata tttcaagcat aacaatcttt
    23461 tgttgttacg ctaacaccga aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa
    23521 aaaaaagccc accacctggc ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg
    23581 taaattacgc ctgctggttc atcagctcgt tgaaagtggt ttctttttca gtcactttcg
    23641 ctttcgccag tacagcttca acagcctgtt cttccagagc aacattgcgc atgttgtcca
    23701 tcagttcttt gtttttgctg tagaactcga taacttcttt cggatcttcg tacgcagaag
    23761 ccatctcttc gatcaggcct ttcacgcgct cttcgtcagc tttcagctcg ttggtgcgga
    23821 taacttcgcc cagcagcagg ccaacaacta cgcggcgttt agcctgttct tcgaacagtt
    23881 cgcgcggcag ttccagagct tgtttttcgt tgccaccgaa acgctgtgca gcctggcgac
    23941 gcagaacgtc gatttcgctg tcgatcagcg cagccggtac gtcgatgtcg ttagctttta
    24001 ccagaccttc gatcgcctga gacttaacgc ggttacggat ggcgctcttc agctcgcgct
    24061 ccatgttttt acgcacttca gcgcgcagac cttctacgga accatcttca acgccgaaac
    24121 gtttgatgaa ttctgcagtc agttccggca gttcacgctc ttcaactttc ttcaggttga
    24181 tagcgaattt cgctgcttta cctttcaggt tttctgcgtg gtattcttcc gggaaggtca
    24241 cgtcgatggt gaactcttcg ccagctttgt ggcctttgat accgtcttca aagcccggga
    24301 tcatacgacc ctggcccatc gccagtacga aatcagacgc tttaccgcct tcgaactctt
    24361 cgccgtctac agaaccggtg aagtcgatgg ttacgcggtc ttctgcttca acagcgccgt
    24421 ctttttcttt ccaggtcgcc tgctgtttac gcagagtatc cagcatgccg tcaacgtcag
    24481 cgtcggtcac ttcaacgatc ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa
    24541 cttccggata aacttcaaac tctacagagt aagtgaagtc ttcacccagc ttgtattcgc
    24601 ccggaacata agtcggtgcg ccagccggat tgattttttc tttaatgatg gcgtcaatga
    24661 agttacggct catcaggtca cccagaacgt cctggcgtac agacgcgcca taacgctgag
    24721 caacgatatt cattggcact ttgcctttgc ggaagccgtc aatacgtact tttttcgcaa
    24781 cgttgaccag ctcgctttta acagcggtct cgatgctgtc agcagcgata gtaatcgtta
    24841 cacggcggcc aaggccttga gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa
    24901 tcacagtgct cggtcaactc ttaccccgca agcacaggtt gttggcttcg cggaaccggg
    24961 atgttcttat aatcaatcac attccctgtt accagaatca tcccgaaagc attcaaatag
    25021 gacgcggcat tatagcggca tcacttttat gagtcgagaa cggttattgc gcgctgtcgc
    25081 gtattttttc acaattcccg ggcaattttc gtctgaatgc tgggcaaaaa gcgcaaaatt
    25141 cagacaaaac aaaacggccc gtaggccgtt tttcataaaa tctttagcaa catactggaa
    25201 aagcgccgac agttgcaaat gcgtttttac gcgatgcttc ctgctccacg acagggagga
    25261 gaggcaaaga cggtgtcctg caacccttcc cactccttaa tagtgtaagt atgcagagcc
    25321 agcgcatgaa cggtagtaga gagttcctcc gctaaagtac tgtaaatcat tcgatgacga
    25381 ttcagaaaac gttcacccgt aaaacgatcg ctgaccagca caactttaaa atggctttca
    25441 gagccggctg ggacattgtg acgatagctt tcatccacta cttcgaggaa tacgggttgg
    25501 aacgccgccc ttaatttttc ttctatccgc tcacgtatca tcatgaaata tctcctccga
    25561 caacgctgag atgtcatcca tccctttaaa tactagccgc ttttaccgtt tccattgcag
    25621 cttaacaaca aatatttagg tttcgtcctg gtttttccgt caagcgtatg aagtttactg
    25681 atttaaagat tgaaacacag gcctcgcatc agtgtttctt ttgccagggg cgattatttc
    25741 gtcaacatga tgaatttaca tgccttaccc acttcccctc gccgttggca atgttatgat
    25801 ggcgggaatt tttaccaaac acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa
    25861 atcctcttcc cgttagttgc tctgtttatg cttgcaggat gcgcaaaacc gccaacaact
    25921 attgaagttt ccccgacgat tacactgcca cagcaggatc caagcctgat gggcgtcacc
    25981 gtaagcatta atggtgccga tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat
    26041 aatcaaatcg ttaccctgac cgcctcccgc gatctgcgtt tcctgctgca agaagtgctg
    26101 gaaaaacaga tgaccgcgcg tggttacatg gttggtccga atggcccggt taatctgcaa
    26161 atcatcgtta gccaactgta tgctgacgtg tcccagggca acgtgcgcta taacatcgct
    26221 accaaagcgg atatcgccat catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac
    26281 tatcgtgcca gctacaacgt tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat
    26341 gcggttaaca gtgtgctgac cgataccatc gctgatatgt ctcaggacac cagcattcac
    26401 gaatttatca agcagaacgc gcgttaattt ctgccctctg gcccggtgca aaccgggcct
    26461 gtagacgccc atgtccagtc aatatttacg tatttttcaa cagccgcgtt cagccatatt
    26521 gctgatcctg ggttttgctt ccgggctacc gctcgccctg acatccggta ccttacaggc
    26581 ctggatgacg gtcgagaata ttgatctcaa aaccattggt ttcttctctc tggtaggcca
    26641 ggcttacgtt tttaaattcc tctggtcacc gctgatggac cgctacacgc ccccattttt
    26701 tgggcggcgg cgcggttggc tgctcgccac gcaaatcctg ttattagtcg ccattgcggc
    26761 gatgggtttt ctcgaacctg gcacccaact ccgctggatg gcggcactgg cggtggtgat
    26821 cgctttttgc tcggcctccc aggatattgt cttcgatgcg tggaaaaccg atgtgctccc
    26881 ggcagaagaa cgtggtgcgg gcgcggcaat cagcgtgctg ggttaccgtt tagggatgct
    26941 ggtttccggc ggcttagccc tgtggctggc agataaatgg ctgggctggc agggcatgta
    27001 ctggttgatg gcggcgctgt tgatcccttg tattatcgcg acgttgcttg caccagaacc
    27061 gaccgacacc attcctgtgc caaaaacgct ggaacaagcg gttgttgcac ctctgcgaga
    27121 tttctttggt cgcaataatg cctggcttat tttgcttctt atcgtgctgt ataagctggg
    27181 cgacgcattc gccatgagcc tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc
    27241 gggtgaagta ggcgtggtta acaaaacgct tggcttactg gcgaccattg tgggcgcatt
    27301 gtacggtggg attttgatgc agcgcctgtc gctgttccgg gcgctgctga ttttcggcat
    27361 tttacaaggt gcgtctaacg ctggttactg gctgctgtcg attactgata agcatctcta
    27421 cagcatgggc gcagccgtct ttttcgaaaa cctctgtggc gggatgggca catcagcctt
    27481 tgtcgcgctg ttaatgacgc tgtgtaataa gtcattttcc gcaactcaat ttgccctgct
    27541 ctcagcgctt tccgctgtag gacgagttta tgtcggcccc gtggcgggtt ggtttgttga
    27601 agcacacggc tggtcgacat tctatctatt ctccgtcgcc gctgccgtac cggggcttat
    27661 tttgctgctg gtttgccgcc agacgcttga atatacacga gtaaatgaca actttatctc
    27721 ccgtaccgaa tatccggcag gttatgcctt tgccatgtgg acactggcgg cgggcgtcag
    27781 cctgttggcc gtgtggttac tgctgttgac gatggacgcg ctggatttga cgcacttctc
    27841 tttcctgcct gctctgctgg aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg
    27901 tggtttgctg gattatctgg cgctacgaaa aacgcatctg acgtaatctg taaatattat
    27961 ttagctgtag taatcactcg ccgtaattat tacggctaaa taaacatcag tatcgcttaa
    28021 taatatttgt tcttttgtgc ggcttagcgt ttggaaatta ttgacgcaat ttatgaattc
    28081 tattttttct tacacgattc agctaatgag tctttatttt ccccccaccc aattgtcacc
    28141 ataatgataa ttatttgtta aacaattgtt ttattccaga ttggttatac caattgcccg
    28201 tccaaactcc ttctgcactc ccctttcgtt ataacgccct tttgcaacag cttcttaaaa
    28261 tcaacttgat atgttttgca acatatgtga cctgacagcc aaatccaagt aacaggaatt
    28321 taatcatgtt tacagtaatg taaccttccc gtaaaatgcc cacacacttt aaacgccacc
    28381 agatcccgtg gaattgaggt cgttaaatga gactcaggaa atacaataaa agtttgggat
    28441 ggttgtcatt atttgcaggc actgtattgc tcagtggctg taattctgcg ctgttagatc
    28501 ccaaaggaca gattggtctg gagcaacgtt cactgatact gacggcattt ggcctgatgt
    28561 tgattgtcgt tattcccgca atcttgatgg ctgttggttt cgcctggaag tatcgtgcga
    28621 gcaataaaga tgctaagtac agcccgaact ggtcacactc caataaagtg gaagctgtgg
    28681 tctggacggt acctatctta atcatcatct tccttgcggt actgacctgg aaaaccactc
    28741 acgctcttga gcctagcaag ccgctggcac acgacgagaa gcccattacc atcgaagtgg
    28801 tttccatgga ctggaaatgg ttcttcatct acccggaaca gggcattgct accgtgaatg
    28861 aaatcgcttt cccggcgaac actccggtgt acttcaaagt gacctccaac tccgtgatga
    28921 actccttctt cattccgcgt ctgggtagcc agatttatgc catggccggt atgcagactc
    28981 gcctgcatct gatcgccaac gaacccggta cttatgacgg tatctccgcc agctacagcg
    29041 ggccgggctt ctcaggcatg aagttcaaag ctattgcaac accggatcgc gccgaattcg
    29101 accaatgggt cgcaaaagcg aaacagtcgc cgaacaccat gtctgacatg gctgcgttcg
    29161 aaaaactggc cgcgcctagc gaatacaacc aggtggaata tttctccaac gtgaaaccag
    29221 acttgtttgc cgatgtgatt aacaagttta tggctcacgg taagagcatg gacatgaccc
    29281 agccagaagg tgagcacagt gcacacgaag gtatggaagg catggacatg agccacgcgg
    29341 aatccgccca ttaaaggggt tgaggaagaa taaagatgtt cggaaaatta tcacttgatg
    29401 cagtcccgtt ccatgaacct atcgtcatgg ttacgatcgc tggcattatt ttgggaggtc
    29461 tggcgctcgt tggcctgatc acttacttcg gtaagtggac ctacctgtgg aaagagtggc
    29521 tgacctccgt cgaccataaa cgcctcggta tcatgtatat catcgtggcg attgtgatgt
    29581 tgctgcgtgg ttttgctgac gccattatga tgcgtagcca gcaggctctt gcctcggcgg
    29641 gcgaagcggg cttcctgcca cctcaccact acgatcagat cttcaccgcg cacggcgtga
    29701 ttatgatctt cttcgtggcg atgcctttcg ttatcggtct gatgaacctg gtggttccgc
    29761 tgcagatcgg cgcgcgtgac gttgcgttcc cgttcctcaa caacttaagc ttctggttta
    29821 ctgttgttgg tgtgattctg gttaacgttt ctctcggcgt gggcgaattt gcgcagaccg
    29881 gctggctggc ctatccaccg ctatcgggaa tagagtacag tccgggagtc ggtgtcgatt
    29941 actggatatg gagtctccag ctatccggta taggtacgac gcttaccggt atcaacttct
    30001 tcgttaccat tctgaagatg cgcgcaccgg gcatgaccat gttcaagatg ccagtattta
    30061 cctgggcatc actgtgcgcg aacgtactga ttattgcttc cttcccaatt ctgacggtta
    30121 ccgtcgcgtt gttgaccctg gatcgctatc tgggcaccca tttctttacc aacgatatgg
    30181 gtggcaacat gatgatgtac atcaacctga tttgggcctg gggccacccg gaagtttaca
    30241 tcctgatcct gcctgttttc ggtgtgttct ccgaaattgc ggcaaccttc tcgcgtaaac
    30301 gtctgtttgg ttatacctcg ctggtatggg caaccgtctg tatcaccgtg ctgtcgttca
    30361 tcgtttggct gcaccacttc tttacgatgg gtgcgggcgc gaacgtaaac gccttctttg
    30421 gtatcaccac aatgattatc gccatcccga ccggggtgaa gatcttcaac tggctgttca
    30481 ccatgtatca gggccgcatc gtgttccatt ctgcgatgct gtggaccatc ggttttatcg
    30541 tcactttctc ggtgggcggg atgactggcg tgctgctggc agtaccgggc gcggacttcg
    30601 ttctgcataa cagcctgttc ctgattgcgc acttccataa cgtgatcatc ggcggcgtgg
    30661 tcttcggctg cttcgcaggg atgacctact ggtggcctaa agccttcggt ttcaaactga
    30721 atgaaacctg gggtaaacgc gcgttctggt tctggatcat cggcttcttc gttgccttta
    30781 tgccgctgta tgcgctgggc ttcatgggca tgacccgtcg tttgagccag cagattgacc
    30841 cgcagttcca caccatgctg atgattgcag ctagcggtgc agtactgatt gcgctgggta
    30901 ttctctgcct cgttattcag atgtacgttt ctattcgcga ccgcgaccag aaccgtgacc
    30961 tgactggcga cccgtggggt ggccgtacgc tggagtgggc aacctcttcc ccgcctccgt
    31021 tctataactt tgccgttgtg ccgcacgttc acgagcgtga tgcattctgg gaaatgaaag
    31081 agaaaggcga agcgtataaa aagcctgacc actatgaaga aattcatatg ccgaaaaaca
    31141 gcggtgcagg tatcgtcatt gcagctttct ccaccatctt cggtttcgcc atgatctggc
    31201 atatctggtg gctggcgatt gttggcttcg caggcatgat catcacctgg atcgtgaaaa
    31261 gcttcgacga ggacgtggat tactacgtgc cggtggcaga aatcgaaaaa ctggaaaacc
    31321 agcatttcga tgagattact aaggcagggc tgaaaaatgg caactgatac tttgacgcac
    31381 gcgactgccc acgcgcacga acacgggcac cacgatgcag gcggaaccaa aatcttcgga
    31441 ttttggatct acctgatgag cgactgcatt ctgttctcaa tcttgtttgc tacctatgcc
    31501 gttctggtga acggcaccgc aggcggcccg acaggtaagg acattttcga actgccgttc
    31561 gttctggttg aaactttctt gctgttgttc agctccatca cctacggcat ggcggctatc
    31621 gccatgtaca aaaacaacaa aagccaggtt atctcctggc tggcgttgac ctggttgttt
    31681 ggtgccggat ttatcgggat ggaaatctac gaattccatc acctgattgt taacggcatg
    31741 ggtccggatc gcagcggctt cctgtcagcg ttctttgcgc tggtcggcac gcacggtctg
    31801 cacgtcactt ccggtcttat ctggatggcg gtgctgatgg tacaaatcgc tcgtcgcggc
    31861 ctgaccagca ctaaccgtac ccgcatcatg tgcctgagcc tgttctggca cttcctggat
    31921 gtggtttgga tctgtgtgtt cactgttgtt tatctgatgg gggcgatgta atgagtcatt
    31981 ctaccgatca cagcggcgcg tcccatggca gcgtaaaaac ctacatgaca ggctttatcc
    32041 tgtcgatcat tctgacggtg attccgttct ggatggtgat gacaggggct gcctctccgg
    32101 ccgtaattct gggaacaatc ctggcaatgg cagtagtaca ggttctggtg catctggtgt
    32161 gcttcctgca catgaatacc aaatcagatg aaggctggaa catgacggcg tttgtcttca
    32221 ccgtgctaat catcgctatc ctggttgtag gctccatctg gattatgtgg aacctcaact
    32281 acaacatgat gatgcactaa gagcggcggt tatgatgttt aagcaatacc tgcaagtaac
    32341 gaaaccaggc atcatctttg gcaacctgat ctcggtgatt gggggattcc tgctggcctc
    32401 aaagggcagc attgattatc ccctgtttat ctacacgctg gttggggtgt cactggttgt
    32461 ggcgtcgggt tgtgtgttta acaactacat cgacagggat atcgacagaa agatggaaag
    32521 gacgaagaat cgggtgctgg ttaaaggcct gatctctcct gctgtctcgc tggtgtacgc
    32581 caccttgctg ggtattgctg gctttatgct gctgtggttt ggcgcgaatc cgctggcctg
    32641 ctggctgggg gtgatgggct ttgtggttta tgtcggcgtc tatagcctgt acatgaaacg
    32701 ccactcagtc tacggcacat tgattggttc gctctccggc gctgcgccgc cggtgattgg
    32761 ctactgtgca gtaaccggcg agttcgatag cggcgcagcg atcctgctgg ctatcttcag
    32821 cctgtggcag atgcctcact cctatgccat cgccattttc cgctttaagg attaccaggc
    32881 ggcaaacatt ccggtattgc cagtggtaaa aggcatttcg gtggcgaaga atcacatcac
    32941 gctgtatatc atcgcctttg ccgttgccac gctgatgctc tctcttggcg gttacgctgg
    33001 atataaatat ctggtggtcg ccgcggcggt tagcgtctgg tggttaggta tggctctgcg
    33061 cggttataaa gttgctgatg acagaatctg ggcgcgcaag ctgttcggct tctctatcat
    33121 cgccatcact gccctctcgg tgatgatgtc cgttgatttt atggtaccgg actcgcatac
    33181 gctgctggct gctgtgtggt aacaaaacct ctctattaaa aaggtgctac ggcacctttt
    33241 ttattgtctg agatcagaga tacggctaat aaccgtatgt cttgacagag tgagcagatg
    33301 aacataaatt aatcatacgg ttgatagccg tattatgtat gagatcgact ccctacgatt
    33361 tcattcagtt aatcaaagag gtcataatgc ctggaaaaac agccactcta cccgatgtcg
    33421 ataaaacgct gaaaaatgcg cgggtcgaac tcaaaactag tcccgacgcc aaaaacaaac
    33481 ttcgtgaagc tgcgcaagcc gttggggtag atttaagcgc ttttattttg agcgctgcta
    33541 tggaacgcgc cgaaagtgtg cttgataacc aacgtcgccg tgagctttcg aatcaaagct
    33601 gggaactgat gaaccaactc atcgctgaac ctgctcaacc gacgctcgcc ctcaaggcgt
    33661 taatgaaaag gaaaaacagc gatggtcgac aagcatgaag agattactct gcccatagtc
    33721 ctctcctgta attatcagtc tgatattact tatcctgggc aaaaacagtt tgattgcggt
    33781 aaccctgtta tcgataaatt tgtacgcgca tcgctaaaga aaagtgtgcg taatagcgac
    33841 tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgatcggcat ctgtactttt
    33901 acggcatatt cgctggaaaa acaacgcgtt tctggcgtcc ttcagggttc acaaccttca
    33961 gaaattggtg ttgtcagatt agtcatgttg ggggtagcac ggaagtatca aaagcagggc
    34021 tttggtcagg acctactatg tgattttttt gaacatgtaa aaataattca ccaggcatta
    34081 ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaattttta tgctcgtctc
    34141 ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat gtttttggcg
    34201 attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa gttttgcctc
    34261 agccgggggc tgagcagcgt taccactttt gttcaattgc ccccgctttc agcaactcca
    34321 catccttgcc gttaatggtt gccgttgcgg caacgattgt ggtccttaca gttgcacaag
    34381 attttcgctc aaattccggt ttataaagaa taagagacga attactggta aatgaaccaa
    34441 ataggtaatc agtaaagcta atattcagcc ggtttaaacc gaaaggcgcg aagccaaccg
    34501 tttggttaat ctccttaccg gatgggcgac gaatgctgaa tgtcacgcga agattggcca
    34561 cttcgcctaa tttattggtg gtggcgaaga ggctatccat gcaggaaaaa cctttatcta
    34621 aaaaattatc tcgcatccca gaacgggtaa ccgtaatcac gggtaacgta tcgtcaaccg
    34681 tttttctgtc aatcatgtct tttcgacaag tagccagaaa gtcttccggg ccaccataaa
    34741 tgtggacacc tttatcaaga tattccttcg ctttcttaca atctacctca acatactgac
    34801 cattggcgta cataatgccc aacctggacc atgctaaatt cacaccgtgc tgcgcggcct
    34861 gattaaatag tgcatacgct ttggcataat ccacccgcac ctgctcaccg tcgtagtaga
    34921 taccccccag cgctaacgga gcttctctgt caccttgttt agccgctaac tcgaaccatt
    34981 taatcgcttc ctgaacatca ggtttggtat cataacgagg ctgataatat tcccagccta
    35041 acaggctctg cgcgtcgata tctcccgatt cagccatttt atgcagtgta tttataacat
    35101 cgggagcagg tttaccaccg tatatgctgt catacaattc atgcgctttc tcgctggcca
    35161 ccacgcttgg tgaaactatc agcattacag aaaataatat
//
LOCUS       DAZFWP020000037        34859 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFWP020000037 DAZFWP020000000
VERSION     DAZFWP020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.037"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(110..862)
                     /gene="tcyN"
                     /locus_tag="ACHK0Z_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             complement(110..862)
                     /gene="tcyN"
                     /locus_tag="ACHK0Z_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU4501671.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(859..1527)
                     /gene="tcyL"
                     /locus_tag="ACHK0Z_RS08165"
                     /old_locus_tag="pgaptmp_001633"
     CDS             complement(859..1527)
                     /gene="tcyL"
                     /locus_tag="ACHK0Z_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU4501672.1"
                     /translation="MQESIQLVIDSLPFLLKGAEYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1542..2528)
                     /gene="dcyD"
                     /locus_tag="ACHK0Z_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             complement(1542..2528)
                     /gene="dcyD"
                     /locus_tag="ACHK0Z_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU4501673.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2633..3433)
                     /gene="tcyJ"
                     /locus_tag="ACHK0Z_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             complement(2633..3433)
                     /gene="tcyJ"
                     /locus_tag="ACHK0Z_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4501674.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3521..4072)
                     /gene="fliZ"
                     /locus_tag="ACHK0Z_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             complement(3521..4072)
                     /gene="fliZ"
                     /locus_tag="ACHK0Z_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU4501675.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTAREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4118..4837)
                     /gene="fliA"
                     /locus_tag="ACHK0Z_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             complement(4118..4837)
                     /gene="fliA"
                     /locus_tag="ACHK0Z_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU4501676.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5002..6495)
                     /locus_tag="ACHK0Z_RS08190"
                     /old_locus_tag="pgaptmp_001638"
     CDS             complement(5002..6495)
                     /locus_tag="ACHK0Z_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HGU4501677.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6750..8162
                     /gene="fliD"
                     /locus_tag="ACHK0Z_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             6750..8162
                     /gene="fliD"
                     /locus_tag="ACHK0Z_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4501678.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8177..8587
                     /gene="fliS"
                     /locus_tag="ACHK0Z_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             8177..8587
                     /gene="fliS"
                     /locus_tag="ACHK0Z_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4501679.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8587..8952
                     /gene="fliT"
                     /locus_tag="ACHK0Z_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             8587..8952
                     /gene="fliT"
                     /locus_tag="ACHK0Z_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU4501680.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9030..10517
                     /gene="amyA"
                     /locus_tag="ACHK0Z_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             9030..10517
                     /gene="amyA"
                     /locus_tag="ACHK0Z_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4501681.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10551..10964)
                     /gene="yedD"
                     /locus_tag="ACHK0Z_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             complement(10551..10964)
                     /gene="yedD"
                     /locus_tag="ACHK0Z_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU4501682.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11151..12356
                     /gene="yedE"
                     /locus_tag="ACHK0Z_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             11151..12356
                     /gene="yedE"
                     /locus_tag="ACHK0Z_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU4501683.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12353..12586
                     /gene="yedF"
                     /locus_tag="ACHK0Z_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             12353..12586
                     /gene="yedF"
                     /locus_tag="ACHK0Z_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU4501684.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12695..13363
                     /gene="yedK"
                     /locus_tag="ACHK0Z_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             12695..13363
                     /gene="yedK"
                     /locus_tag="ACHK0Z_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU4501685.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(13756..14070)
                     /gene="fliE"
                     /locus_tag="ACHK0Z_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             complement(13756..14070)
                     /gene="fliE"
                     /locus_tag="ACHK0Z_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4501686.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14285..15943
                     /gene="fliF"
                     /locus_tag="ACHK0Z_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             14285..15943
                     /gene="fliF"
                     /locus_tag="ACHK0Z_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4501687.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMMQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15936..16931
                     /gene="fliG"
                     /locus_tag="ACHK0Z_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             15936..16931
                     /gene="fliG"
                     /locus_tag="ACHK0Z_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4501688.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16924..17610
                     /gene="fliH"
                     /locus_tag="ACHK0Z_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             16924..17610
                     /gene="fliH"
                     /locus_tag="ACHK0Z_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4501689.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17610..18983
                     /gene="fliI"
                     /locus_tag="ACHK0Z_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             17610..18983
                     /gene="fliI"
                     /locus_tag="ACHK0Z_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4501690.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            19002..19445
                     /gene="fliJ"
                     /locus_tag="ACHK0Z_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             19002..19445
                     /gene="fliJ"
                     /locus_tag="ACHK0Z_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4501691.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19442..20569
                     /gene="fliK"
                     /locus_tag="ACHK0Z_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             19442..20569
                     /gene="fliK"
                     /locus_tag="ACHK0Z_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4501692.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            20674..21138
                     /gene="fliL"
                     /locus_tag="ACHK0Z_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             20674..21138
                     /gene="fliL"
                     /locus_tag="ACHK0Z_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU4501693.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21143..22147
                     /gene="fliM"
                     /locus_tag="ACHK0Z_RS08275"
                     /old_locus_tag="pgaptmp_001655"
     CDS             21143..22147
                     /gene="fliM"
                     /locus_tag="ACHK0Z_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU4501694.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22144..22557
                     /gene="fliN"
                     /locus_tag="ACHK0Z_RS08280"
                     /old_locus_tag="pgaptmp_001656"
     CDS             22144..22557
                     /gene="fliN"
                     /locus_tag="ACHK0Z_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU4501695.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22560..22925
                     /gene="fliO"
                     /locus_tag="ACHK0Z_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             22560..22925
                     /gene="fliO"
                     /locus_tag="ACHK0Z_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU4501696.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22925..23662
                     /gene="fliP"
                     /locus_tag="ACHK0Z_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             22925..23662
                     /gene="fliP"
                     /locus_tag="ACHK0Z_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4501697.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23672..23941
                     /gene="fliQ"
                     /locus_tag="ACHK0Z_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     CDS             23672..23941
                     /gene="fliQ"
                     /locus_tag="ACHK0Z_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU4501698.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23950..24735
                     /gene="fliR"
                     /locus_tag="ACHK0Z_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             23950..24735
                     /gene="fliR"
                     /locus_tag="ACHK0Z_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4501699.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25025..25648
                     /gene="rcsA"
                     /locus_tag="ACHK0Z_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             25025..25648
                     /gene="rcsA"
                     /locus_tag="ACHK0Z_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4501700.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(25692..25880)
                     /gene="dsrB"
                     /locus_tag="ACHK0Z_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             complement(25692..25880)
                     /gene="dsrB"
                     /locus_tag="ACHK0Z_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU4501701.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26043..26270
                     /gene="yodD"
                     /locus_tag="ACHK0Z_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             26043..26270
                     /gene="yodD"
                     /locus_tag="ACHK0Z_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU4501702.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26568..27383
                     /gene="yedP"
                     /locus_tag="ACHK0Z_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             26568..27383
                     /gene="yedP"
                     /locus_tag="ACHK0Z_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU4501703.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(27380..29074)
                     /gene="dgcQ"
                     /locus_tag="ACHK0Z_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             complement(27380..29074)
                     /gene="dgcQ"
                     /locus_tag="ACHK0Z_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU4501704.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPFNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(29245..29427)
                     /gene="yodC"
                     /locus_tag="ACHK0Z_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             complement(29245..29427)
                     /gene="yodC"
                     /locus_tag="ACHK0Z_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU4501705.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(29506..30423)
                     /gene="yedI"
                     /locus_tag="ACHK0Z_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             complement(29506..30423)
                     /gene="yedI"
                     /locus_tag="ACHK0Z_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU4501706.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            30596..31516
                     /gene="yedA"
                     /locus_tag="ACHK0Z_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             30596..31516
                     /gene="yedA"
                     /locus_tag="ACHK0Z_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU4501707.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(31505..31975)
                     /gene="vsr"
                     /locus_tag="ACHK0Z_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             complement(31505..31975)
                     /gene="vsr"
                     /locus_tag="ACHK0Z_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU4501708.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(31956..33374)
                     /gene="dcm"
                     /locus_tag="ACHK0Z_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     CDS             complement(31956..33374)
                     /gene="dcm"
                     /locus_tag="ACHK0Z_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU4501709.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDSRRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDEADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33441..34136)
                     /gene="yedJ"
                     /locus_tag="ACHK0Z_RS08355"
                     /old_locus_tag="pgaptmp_001671"
     CDS             complement(33441..34136)
                     /gene="yedJ"
                     /locus_tag="ACHK0Z_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU4501710.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQGAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYELDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(34176..34457)
                     /gene="drpB"
                     /locus_tag="ACHK0Z_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             complement(34176..34457)
                     /gene="drpB"
                     /locus_tag="ACHK0Z_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HGU4501711.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         8522 a         9060 c         8811 g         8466 t
ORIGIN      
        1 gcattctctt atgttattaa tgagttatgc tgatttgtta agcagtttta tcacgcttga
       61 aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat tttttcttat tattgcagca
      121 gaaacttctc gaggaactgg cgggtgcgag gctgctgggg atcggcaaat aacgcttttg
      181 ctgccccctg ctcgactatc cgcccctggt ccataaagat cgcccggtcc gcaacatccc
      241 gggcaaagct catttcgtgt gtcacaatca ccatcgtgcg cttttcctgc gccagctggc
      301 ggatggtgtt caggacttcg cccaccagct ctggatccag cgccgacgtt ggctcgtcaa
      361 acaaaatcac ctcaggacgc attgccagtg cacgcgcaat cgcaacacgc tgctgttgac
      421 cgccagacaa acgacgtgga tagctggttt ctttaccggc cagcccaact ttcgccagca
      481 gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc accggccctt
      541 caataatgtt ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg aagacaaacc
      601 cgacgtgctg acgcaactgg cgaatcagcg atttttgctg acttaatgaa cgtgcagtat
      661 caatagtgat atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc agatttatgc
      721 tgcgtagcaa cgtggttttg ccggaaccac tcggaccgat aattgccacc acttcgccag
      781 gctttacctc gaggtcgata ccgtgcagca ccgtctgacc gtgaaatttt ttcaccaggt
      841 tcttaacttc aatggcactc attttggttc tctctcctgg cgattaagtt gattctcaaa
      901 atggttctgc aacgtcgata atactgtcgc catgatccag tagatgagcg aagccgccag
      961 gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac tgcgcctgac ggaacagctc
     1021 cggcacctgg attgtcgcgg ccagcgacgt atcttttacc agactgataa agctgttcga
     1081 cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca tggtctgcca
     1141 cggcgtcata ccaatactgg cagcggcttc ccactgacct ttatcaatag aagaaatggc
     1201 ggcgcgcagc gtttcggcgg cataggcggc agtgttcagc gacagaccaa tcattgctga
     1261 cggaatggga tctaattcga taccaaactg cggcaggccg taatagatca taaacagttg
     1321 tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa cgcgccagcc agcgcacggg
     1381 ccagattggc gacaggcgca tcagcgcgag aataaacccg agtagtaagc caaaaaacat
     1441 gccgccgata ctgagttgca gggtatactc agcccctttc aacaggaacg gcaaggaatc
     1501 aataaccagt tgtatacttt cttgcatgag cgttcccgga gctaaacgtg gggatgatag
     1561 gcgaacagcg caggcgcgcc gccggtatga ataaacagaa tcggcccttc atctttgaag
     1621 cgtttctgac tgataccgtc aatcagcccc gccatcgctt ttccggtata cacaggatca
     1681 agcagaatgc cttccagacg cgccagcaat ttcactgctt ccatgccttc gtcgttcggc
     1741 acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa tttccgctga tgcggtcagc
     1801 tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt tcggcaattg atcggcaacg
     1861 gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc cagcccaaca
     1921 gccaatccgg cgtgagttcc ggcactgccc gatgcgacca ccaccgacga aatattaacc
     1981 gccccttcac actgttgcgc gatttccagc gcactctcca cataacccag cgcgcccaga
     2041 gcattagaac cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc gactcgcgtc
     2101 gccagctctt ccagttgggc attgggatcg gtcagtgcgt cgcacatttc aatctgggta
     2161 ttgaacagat ccagcaacaa acgattgccg ttggttaaat agttttctgc ggttgtgcca
     2221 ataggatttt ccagcagcgc cacgcagtgc agaccgagtt tcgccgcgac tgcggcagtc
     2281 tggcgcacat ggttagactg gatcgcgccg gcagtaatca gcgtatcggc accttcgcgc
     2341 agggcatctg ccgcgagaaa ttccagctta cgtaatttat tgccgcccat tgccatgggt
     2401 gtgacgtcat cccgtttgat gaaaatttcc cgtccaagat aatcagaaaa gcgcggcaga
     2461 tattcgagcg gcgttggcgc gccgataaac tccaaccgtg gaaaacgggt taaattatgc
     2521 agtggcataa cagcctccga tgtgtgttgt tgtgattttc ttattatgca cgctgaaaac
     2581 gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg tcattatgct gattatttgg
     2641 tcacatcagc accaaaccat ttttcggaaa gagcttgcag agtgccgtct ttttgcattt
     2701 ccgcaattgc atcattcacc gctttcagca ggtcttcatt tcctttacgc agcgccacgc
     2761 cagactcctg acgggagaat gcttcaccgg ttactgccag cgtatcgttg gttttcttca
     2821 ccagatccag cgccgccaga cgatcaacga ggatcgcatc gatacggcct acgcgcagat
     2881 cctgatattt ggtcgggtca tcatcatagg tacgcacatc gacgccctga acattctgcc
     2941 gcagccactc ttcatagttg gtgcccagac cgacacccac ttttttgcct ttcagatcag
     3001 cagccgtttt aatggtgcct tcgttacctt ttttcaccag cgcctgaata ccggaaatgg
     3061 tgtaaggggt agagaaatcg tattttttct tgcgctcgtc agaaatggtg acctgattaa
     3121 tcaccacatc aatgcgttta gagtccagcg acgccagcat accgtcccat ttggtcggtt
     3181 ttagtgacgc ctcaacgcca agatgttttg ccagctgttg ggcaaattcc acttcaaaac
     3241 cggttaattt gccgtcatct ccctgaaaac tgaacggcgg ataagtgcct tccagcccta
     3301 ccagcagcgt gccgcgctct ttaactttat taagcagacc ttcatctgca aaacttttaa
     3361 cgctcatgcc cgcaaccagc gctacggcca tcacacccat caatgcctga cgtcccagat
     3421 gtgctaattt catatttacc ccgaatgttg ttatgtctgt ttgcagtgta gagccattgt
     3481 ttgtaaacac aaaaacaact ccgctacatc ttattcttat ttaatatata tcagaagcag
     3541 gcaggctgga ggatttctgc acaagtccgg tacacgtttg acgctgatag tgttgatact
     3601 tccgtaatgc gatgcggtaa ttgttggtgc tggtggtggg cagccacggg gcgaggattt
     3661 catcaaggaa accgtcctgc agcggatcga gggaaatatt ttgctcgtgt agatgattcc
     3721 ccaaacggcg cagacgaacg acatattcac gcgccgtacc agggctcatt tcagtttgct
     3781 caaaaagaaa ttgcttaaag ccgataatat cgaaaaaatc actctgcgtt ttgcaatgta
     3841 aatcaccgca gaatcggcac aatgccgccc atgatttttg ttccacttgc cagccatttt
     3901 catcaagcag catgtccagg cgggaaatct cgattttatt cactattttg ccgtcgcgaa
     3961 cgagggtaat gcggtcgagt aatttacggc aatgcgcgca atgggtctgg ctgtgtttaa
     4021 agtctttaag atagcggctt aatggccgtc ttttcaggtg ctgcaccatc attaagaact
     4081 cctggtagtc aaaattaaag tgcggcattt actgacgtta taacttaccc agtttagtgc
     4141 gtaaccgttt aatggcctgg ctgtgtaact gactgacccg cgattccccg acctccagca
     4201 ccgcgccaat ctctttgaga ttcagctctt cctggtaata gagggttaat accagttttt
     4261 cgcgctccgg caacgtttcg atggcttcca tcacccgctg gcgcagatta ctgtccagca
     4321 gttgttgtag cgggttttct cgctgatgat catcagtaac cagttcgatg ctatcgccgt
     4381 gctcttcgcg ccactcatcg taggagaaga gctggctgtt attggtgtcg agcaacattt
     4441 ggcgataatc ggcaatatcg atccctaaac gttccgctac ctcagtttcc gtggcgttgc
     4501 ggccaagttc ctgctccagt tgccctattg cctgtgccac ttcacgcgcg ttgcgtcgca
     4561 cgctgcgcgg cacccagtca cggctgcgaa gttcatccag catagcgcca cggatacgct
     4621 gcactgcgta agttgtaaat gccgttcctt gtagggcgtc atagcgttcg acggcattaa
     4681 gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc cacgctcgcg ggcagtcgaa
     4741 cctgcaggcg caatgcttcg tgacgcacca gcgggacata acgctgccac agcgagtgtt
     4801 tatccattac accttcagcg gtatagagtg aattcacgat aaacagccct gcgttatatg
     4861 agttatcggc atgattatcc gtttctgcag ggtttttaat cggacgatta gtgggtgaaa
     4921 tgaggggtta tttgggggtt accggtaaat tccatgcaga aaaaaccccg ccggtggcgg
     4981 ggtttgagcg ataagtgtaa attaacgcag cagagacagt acgttctgcg tggtctggtt
     5041 agcctgtgcc agaacagaag tacctgcttg ttgcaggatc tgcgcacgag acatgttaga
     5101 cacttcggtc gcgtagtcag catcttcgat acggctacgg gcagaagaca ggttgtttac
     5161 ggtgttgcca aggttggtga tggcagagtc gaaacgattt tgaattgcac ctaggttaga
     5221 acgcaacgag tcaacctgag acagtgcttt atcgatagca gccaaaggat cttctgtttt
     5281 ctgcgtagaa attgctgcaa tagaatcagt tttcaggctc ttaccttgta aatcactaac
     5341 actgtaagtt ttgccatcgg ttgcagttac aacttcggtt ttgccatcgc ttccacccag
     5401 ttcaacttta acattgtcgt gtttgacacc gtcagcatca gtgtaagaca ttgttttaac
     5461 ggaaactgct cctgttttct gatcaacatt cgctgcatac tgtttaccgt catcaccaat
     5521 caacgcaaat gaaccagtat ctttgccatt ggcgtcttct aacttaaaga gttttgcacc
     5581 tgtgatagaa gcggttgcgg tatcagtacc caactgaaca tcaatagccc ccttcccttt
     5641 caccgttgtg acttccttta aattaccagc ttgaggctgg gtacccgcta gagcccccag
     5701 cttcagctta cctgcatcag gagatgtcgg atcatcctcc acagtaactt cataataacc
     5761 attttcagcg ggtttatctg aaccggcaac tttaacgtac aacttaccgt tagcatcaga
     5821 cataatatta ccgtcacgtt ggcccggagt cgtagtgcct ttaactgtag ctgtaatatc
     5881 tgcatcaagt gtattagcgt cagcaagctt aactgtcgat ccaaaatagg ttgtttcagc
     5941 cgttgcggct tttaatttag taggatcttt aataccaaaa ccagataatc ccagtgtgtc
     6001 ggaattaatt tcctgcagtt taatagtaat tgtttcgccg tcgtttgcac caacctgaat
     6061 agtcatatcc tgatcagaag ccagcacttt aacgccgtta aactgagtct gaccagaaac
     6121 acggtcaatt tcactcagac gctgctggat ttcggactgg atggaggtca ggtcactgtc
     6181 agagttagta ccgttagttg cctgaacaga aagttcacga atacgctgca ggttgttgtt
     6241 aatttcattc agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg
     6301 ggaagcctga gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc
     6361 tgcgtcatct ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct
     6421 cagagaagac tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt
     6481 aatgacttgt gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc
     6541 ccacggtttc tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt
     6601 agtttctctc ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc
     6661 gcatcatctt cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat
     6721 ctactgtttg caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt
     6781 cgaatctgcc gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa
     6841 acccaattac tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa
     6901 aaagtgcatt agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca
     6961 aaagcactac cgttaccagt agcaatgaag accttaaagt ttccaccgaa gcaggtgctg
     7021 ctcccggtat ctataccatc agcgtaaccc aactggcgca ggcgcaatct ttacgaactg
     7081 attccccgac aatcattgca tcaacaaaag atgcactggg tgatgagtca tcagatacac
     7141 gcacaataaa aattacccaa gacggtcgta aagagccgct ggaaatcaag ctcaataaag
     7201 atcagacgtc attggatgag atcagcaaag ccattaacga tgccgatagt ggaatttccg
     7261 ctagtatcgt taaagtcaaa gatggtaact atcaattggt attgaccgcc agtgaaggtc
     7321 tggccaataa aatgaccata tctgttgagg gtgacagtaa attaaatgat ctgctggctt
     7381 atgacagcaa gaccaacacc gggaatatga aggaactggt taacgcgcaa aatgcacagc
     7441 taaacgttaa cggtatcgat attgaacgtt caagtaacaa aattaccgat gctcctcagg
     7501 gagtgactct cgatttgacc aaaaaagtca ctgacgtccg ggtcacggtg acaaaaagca
     7561 acgataaagc aaccgaagca attaaaggct gggtggattc atataactcc ctcattgaca
     7621 cctttaacac cctgacaaaa tataaagaag tagacccagg tgctgaagca caagataaaa
     7681 ataatggtgc acttctcgga gacagcgttg tacgtacaat tcaaagcggc atccgcgcac
     7741 aatttgcaaa cggtgccagc gacggtgcct tcaaaacatt aaacgagatc gggattaagc
     7801 aggacggcac gacgggtaag ctgaaaattg atgatgataa actcaaaaaa gtcctgaatg
     7861 aaaatacagc atccgtacgt gaattgctgg taggcgatgg taaagaaaca ggaattacca
     7921 ccaaaattgc caccgaggta aaaggttacc ttgccgatga tggcattatt gatagcgcac
     7981 aggacagtat taacgccaca ctgaaaaaac tgaccaaaca gtatctatcc gtcagtgcca
     8041 gcattgatga caccgtggcg cgttacactg cacagtttac gcaattagat accatgatga
     8101 gcaaactgaa taacaccagt acctatctca gccaacaatt tactgcaatg agtaattcct
     8161 gatcacggag gtcagcatgt ataccgcgag cggctcaaaa gcctatgcac aaattggcgt
     8221 cgaaagcgcc gtaatgagcg ccagccagca gcagctggtc accatgctat ttgatggagt
     8281 gctgagcgca ctggttagag cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg
     8341 cgtctctttg tcaaaagcga tcaacatcat tgagaacgga cttcgggtga gtcttgatga
     8401 agagagcaaa gacgaattaa cccaaaactt gattgctctt tatagctata tggtcaggcg
     8461 cttgctgcaa gccaatttac gcaacgatgt ctccgcagtc gaagaagtgg aagcattaat
     8521 gcgtaatatt gccgatgcct ggaaagagtc gttactctcc ccttctttga ttcaggaccc
     8581 agtctgatga accatgcacc gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc
     8641 cagctcatgt tacgcctggc aacggaagaa caatgggacg aactcatcgc cagcgaaatg
     8701 gcgtatgtga atgcggtgca ggagatagca cacctgactg aagaggttgc cccgtccacc
     8761 acgatgcagg agcagctccg cccgatgctg cgcctgattc tcgacaacga aagcaaggta
     8821 aagcagttat tacagattcg tatggatgaa ctggcgaaac tggtcggtca gtcatcggtg
     8881 caaaaatcgg tgttaagtgc ctatggcgat cagggcggct ttgtgctggc tccgcaagat
     8941 aacttctcgt gaatcttaac gagctaatgg ttcgcgaata atccgattac ggctacgctt
     9001 ctaatgttcc ccttgaatgg agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc
     9061 actggtatta cccggaaggc ggtaagctct ggcctgaact ggccgagcgc gccgacggtt
     9121 ttaatgatat tggtatcaat atggtctggt tgccgcccgc ctataaaggc gcatcgggcg
     9181 ggtattcggt cggctacgac tcctatgatt tatttgattt aggcgagttt gatcagaaag
     9241 gcagcatccc tactaaatat ggcgataaag cacaactgct ggccgccatt gacgccctga
     9301 aacgtaatga cattgcggtg ctgttggacg tggtagtcaa ccacaaaatg ggcgcggatg
     9361 aaaaagaagc tattcgcgtg cagcgtgtaa atgctgatga ccgtacgcaa attgacgaag
     9421 aaatcattga gtgtgaaggc tggacgcgtt acaccttccc cgcgcgtgcc gggcaatact
     9481 cgaagtttat ctgggatttc aaatgtttta gcggtatcga ccatatcgaa aaccctgatg
     9541 aagatggcat atttaaaatt gttaacgact acaccggcga aggctggaac gatcaggttg
     9601 atgatgaatt aggtaatttc gattatctga tgggcgagaa tatcgatttt cgcaatcatg
     9661 ccgtgacgga agagattaaa tactgggcgc gctgggtgat ggaacaaacg caatgcgacg
     9721 gttttcgtct tgatgcggtc aaacatattc cggcgtggtt ttataaagag tggatcgaac
     9781 acgtacagga agttgcgcca aagccgctgt ttattgtggc ggagtactgg tcgcatgaag
     9841 tcgataaact gcaaacgtat attgatcagg tggaaggcaa aaccatgctg ttcgatgcgc
     9901 cgctgcagat gaaattccat gaagcatcgc gcatggggcg tgactacgac atgacgcaga
     9961 ttttcacggg tacattagtg gaagccgatc ctttccacgc cgtgacgctc gttgccaatc
    10021 acgacaccca accgttgcaa gccctcgaag cgccggtcga accgtggttt aaaccgctgg
    10081 catatgcctt aattttgttg cgggaaaatg gcgttccttc ggtattctat ccggacctct
    10141 acggtgcgca ttacgaagat gtcggcggtg acgggcaaac ctatccgata gatatgccaa
    10201 taatcgaaca gcttgatgag ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga
    10261 cgttattttt cgaccatccg aactgcattg cctttagccg cagtggcact gacgaatatc
    10321 ccggctgcgt ggtggtcatg tcgaacgggg atgatggcga aaaaaccatt aatctgggag
    10381 agaattacgg caataaaacc tggcgtgatt ttctggggaa ccggcaagag agcgtagtaa
    10441 ccgacgaaaa cggtgaagcg accttctttt gcaacggcgg cagcgtcagc gtgtgggtta
    10501 tcgaagaggt gatttaaatt catccccggc ggcaagccgg ggagatttca ttacggcagt
    10561 ggcgtcggta acggcgcttt atccagcgcg gcggcacact ctgccgtcgg gcgatccaca
    10621 cgctccatcg tcataccatc gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc
    10681 tcacgtttta cggtaacgtt agtgagatca tcagacatca atgtcaactt gcccggcacg
    10741 gcaatcaccc gctgccactg acggcaatcc agggtatcgc cctctttagt gacaatcagg
    10801 ctgcctatcg cttccggact gaccagcgca cgttgcggcc ctttggtctg ccagtagccc
    10861 gccagccagt ccggcgcggg ggttttcacg acattgttat agttttcgac ttcggcacag
    10921 cccgccagca acatgagcgc gcctgcaatt gcgagttttt tcatcattcg tcctgcatga
    10981 gaagaaaaga ggattgtggc attaaagccc tgatgtcgcc agcctttcca ggttcagaga
    11041 gaaatttgat ctaactatta tttttgtgaa ttattacccc gaaaataaat ttgcatgatg
    11101 tatgattcgc gattcttgtt tcaaaccttc tgagttcaga ggctacattc atgtcatggc
    11161 agcaattcaa acacgcctgg ttgattaaat tctgggcacc catccctgcg gtcatcgcgg
    11221 cgggtattct ctccacttac tattttggca ttactggcac cttttgggct gtcacgggtg
    11281 aatttacccg ttggggcggt cagctcctgc agctgtttgg cgtccatgct gaagagtggg
    11341 gttattttaa aattatccat ctggaaggat cgccattaac ccgcatcgac gggatgatga
    11401 tcctcggcat gtttggcggc tgctttgccg cagcgctgtg ggccaacaat gtcaaactgc
    11461 gcatgccacg cagccgtatc cgcattatgc aggccattat tggcggtatt atcgctggtt
    11521 ttggcgcgcg tctggcaatg ggctgtaacc tggcggcgtt ctttaccggt attcctcagt
    11581 tctcgctgca tgcctggttc tttgccatcg ccactgccat tggttcatgg tttggcgcac
    11641 gctttaccct gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct
    11701 caccgctgac gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct
    11761 ttttcggtat gctgggctgg gcgctgctca cggcgatgaa ccaaccaaaa ctggggctgg
    11821 caatgctgtt tggcgtcggc tttggtttac tgattgaacg tgcgcaaatc tgctttactt
    11881 cggcgttccg cgatatgtgg atcaccggac gtacccatat ggcgaaagca atcattatcg
    11941 gtatggcggt gagtgccatc gggatcttca gttacgtaca gttaggcgtt gaacccaaaa
    12001 tcatgtgggc gggaccaaac gcggtaattg gtggtttact gtttggtttt ggcatcgtgc
    12061 tggcaggcgg ctgcgaaacc ggttggatgt accgcgcggt agaaggtcag gtgcactact
    12121 ggtgggtcgg tctgggcaat gtgatcggct caaccattct ggcgtattac tgggatgatt
    12181 tcgctccggc gctggccacc gactgggaca aaatcaacct gctgaaaacc tttggcccga
    12241 tgggtggcct gctggtgaca tatttgctgt tgtttactgc gctgatgttg attatcggct
    12301 gggaaaaacg cttcttccgc cgtgcggcac cgcagactgc taaggagatc gcatgaaaaa
    12361 tatcattcct gattaccgtc ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc
    12421 aacccttgag gcgatgccgc agttaaaaaa aggtgagatc ctggaagtgg tgagcgactg
    12481 tccgcagtcg atcaataata ttccactgga tgctcgtaat cacggctata cggtgctgga
    12541 tattcagcaa gacgggccga ccattcgtta tttaattcag aagtaatccc tcattcccgc
    12601 tgtggccaac agcgggaata tcgttgcctc tgtaccacat agattttacc cgccccctct
    12661 cctattcttt tcagtgtgct ctcaggaggt tgctatgtgt ggacgctttg cccaatccca
    12721 aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctatga
    12781 tcccgaaccc attggcagat acaacgtcgc gccgggaacc aaagttcttc tgctcagtga
    12841 acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga
    12901 taaaccgccg ctgattaacg cccgcgtaga aactgcggcc accagtcgta tgtttaaacc
    12961 gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga
    13021 aggcgacaaa aaacagcctt attttatcta tcgtgctgac ggacaacctg tttttatggc
    13081 cgcgataggt agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt taattgtcac
    13141 tgcagcagca gaccagggtc tggtagatat tcatgaccgc cgcccgctgg tactgtcgcc
    13201 agaagctgcg cgggaatgga tgcggcaaga gattggcggt aaagaagcat cagaaattgc
    13261 taccaacggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgc gcgcggtagg
    13321 taatgtcaaa aaccagggcg cggagttaat tcaaccggtt tgataagcca ggagactatt
    13381 gatcaataat actgcgtcat aagaaatctc tattagacaa agtagacgct ccttccacat
    13441 gcgccacaag aaacctctat tccagtgaca caattacgcc tgattatttc cctagcccgg
    13501 aatatgaata aaaacatcaa caaagattaa attattcaaa cgttactgtt gcgtctgcgg
    13561 tcgtaacaac atccgacgtg gtgatgtgta tcaaatgagg cctctgatga tcagagggtt
    13621 tattgcgtat acggatcgtg gatggcagaa aactttcagg atcaggtatc cattttaaac
    13681 cagaaatagg gtgagtttgc cccatccatg ccccacgcgg gcagatggga tgtaattaac
    13741 aacaggttac aaaacctaca cttgcatgga catcacttcc tgatacgccg ccaccagctt
    13801 attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat cggtcatcac
    13861 atcgtttaac gccacgccgg gttcaccgag ggtgaatttt tctgcctgcg tgcgggcagc
    13921 tgtttgtgta tcgctaatgc gatcgagcgc ggcgtgcagc tgcccggcaa aactaatggt
    13981 cggttgcggc agtgaatcct gcgcgcgcgc actcatcgcc gtagcctgta actggctgat
    14041 aaccccttca atcccctgta tcgctgacat tttcatctcc tggataattt ctggtagcaa
    14101 agcctaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa cgggtttatt
    14161 ggcggataga aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtttgctga
    14221 cccgggagtg agtcttgttc cactttgcca ataacgccgt ccataatcag ccacgaggtg
    14281 cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc gcctgcgtgc
    14341 gaatccgaaa attccattga ttgttgccgg ttccgcggca gtggcggtca tggtcgcact
    14401 gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt ccgatcagga
    14461 tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct tcagcgaagc
    14521 cagcggcgct attgaagttc cggcagataa agttcacgaa ctgcgtctgc gcctggcaca
    14581 acaaggtttg cccaaaggcg gcgcggtcgg tttcgaacta ctcgatcagg aaaagtttgg
    14641 tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg
    14701 taccatcgca actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc
    14761 gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc
    14821 cggccgcgca ctcgatgaag ggcaaattag cgccattgtg catctggttt ccagcgccgt
    14881 tgctggtctg ccgccgggaa acgtcacgct ggtggatcag ggcggacatc tgttaaccca
    14941 gtccaatacc agcgggcgcg atcttaatga tgctcagttg aaatatgcca gcgatgtcga
    15001 aggccgtatt cagcggcgta ttgaagcaat cctgtcgcct attgttggta acggtaatat
    15061 tcacgcccag gtcacggcgc agctggactt cgccagtaaa gaacaaacgg aagaacagta
    15121 tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta atgagagcga
    15181 gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc
    15241 tgcgaataac gcgccaatca gcacgcctcc agcaaatcaa aataaccgcc agcagcaggc
    15301 gagcaccacc agcaatagcg ggccgcgtag cacacagcgg aatgaaacca gtaactacga
    15361 agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt
    15421 cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc tcagcaacga
    15481 acagatgatg caaattgaag atctgacccg cgaggcgatg ggcttttctg aaaaacgcgg
    15541 tgactcgctc aatgtcgtta actcgccgtt caatagcagt gacgaaagcg gcggagaact
    15601 gccgttctgg caacagcaag cgtttatcga tcagttgctg gctgccggtc gctggttgct
    15661 ggtactgctg gtggcgtggc tgctgtggcg gaaagcggta cgcccgcagt taacacgtcg
    15721 cgccgaggcg atgaaagctg tacagcaaca ggcgcaggcc cgtgaggaag tggaagatgc
    15781 ggtggaagtc cgcctgagca aagacgaaca actccaacaa cgacgcgcta accaacgtct
    15841 gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc cgcgcgtggt
    15901 ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca ggcaccgata
    15961 aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg ttcaagcacc
    16021 tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg cagatctcca
    16081 acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag tttgccgcac
    16141 tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg ggtgaggaac
    16201 gtgccgccag cctgctggaa gatattctcg aaactcgcga taccgccagc ggtattgaaa
    16261 cgctcaactt tatggagccg cagagtgccg ccgatctgat tcgcgatgag catccgcaaa
    16321 ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt ctggcgttgt
    16381 tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc ggcgtgcagc
    16441 cagccgcgct ggcggaactg accgaagtac tgaatggctt gctcgacggt cagaatctca
    16501 agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg atgaaaactc
    16561 agcaggaaga agccgttatt accgccgtgc gcgaattcga cggcgagctg gcgcaaaaaa
    16621 ttatcgacga gatgttcctg ttcgaaaatc tggtggatgt cgacgatcgc agcattcagc
    16681 gtctgttgca ggaagtggat tccgaatcgc tgttgatcgc gctgaaaggg gccgagcagc
    16741 cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt ctgcgcgacg
    16801 atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtgga aaacgaacag aaagcgattc
    16861 tgctgattgt gcgccgcctt gccgaaactg gcgagatggt aattggcagc ggcgaggata
    16921 cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc gccaccacag
    16981 gcagagtttg tgcccatggt cgagccggaa gaaaccatca ttgaagaggc cgaacccagc
    17041 cttgagcagc aactggcgca actgcaaatg caggcccatg agcaaggtta tcaggcgggg
    17101 attgccgaag gtcgccagca aggtcatgag cagggctatc aggaaggact ggcccggggg
    17161 ctggagcaag gtctggcaga ggcgaagtct caacaagcgc caattcatgc ccggatgcag
    17221 caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat tgcttcgcgc
    17281 ctgatgcaga tggcgctgga ggcggcacgt caggtcatcg gtcagacgcc aacagtggat
    17341 aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt attcagcggt
    17401 aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg tggatgatat gctcggcgca
    17461 accttaagtt tgcatggctg gcgcttgcgg ggcgatccca cactccatcc tggcggctgt
    17521 aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg gcaagaactc
    17581 tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc tggctaacca
    17641 cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc tatgggcgat
    17701 taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg ctcggcgcaa
    17761 cctgtgtcat tgaacgccag aacggcagcg aaacgcacga agtagaaagc gaagtcgttg
    17821 gctttaacgg tcaacggctg tttttaatgc cgctggagga agtcgaaggt gtcctgcccg
    17881 gcgcgcgggt ttatgccaaa aacatttcgg ctgaaggact gcaaagcggc aagcagttgc
    17941 cgctcggtcc ggcgttatta ggtcgcgttc tggacggcag cggtaagccg ctcgatggcc
    18001 tgccctcccc cgatacgacg gaaaccggtg cgctgattac cccgccattt aacccgttgc
    18061 aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat gccctgctta
    18121 ccgttgggcg tgggcagcgt atgggactgt ttgccgggtc cggcgttggt aaaagtgtgc
    18181 tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt ttgattggtg
    18241 aacgcggacg cgaagtaaaa gattttattg agaatatcct cggtgccgaa gggcgtgcac
    18301 gctccgtggt gattgccgct ccggcggatg tttctcctct cctgcgaatg cagggtgccg
    18361 cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg ttgctgatta
    18421 tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg attggcgaac
    18481 cacccgccac taaaggttat cccccgtcgg tgtttgccaa attaccggca ctggtcgagc
    18541 gtgccggaaa tggcattagc ggcggcggat cgattactgc attttatacc gtgctcactg
    18601 aaggcgatga ccagcaggac cccattgccg actctgcgcg ggcaatcctc gacggtcaca
    18661 ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat attgaagcgt
    18721 cgatcagccg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga gtgcgcacct
    18781 tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc ggcgcgtatg
    18841 ccaaaggcag cgatccgatg ctcgacaaag ccatcgccct gtggccgcag ctggagggct
    18901 atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag gggctggagc
    18961 gtattttccc gacagtgtca taacccagga gataacggca gatggcagaa catggcgcgc
    19021 tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgcgcgc ctgctgggtg
    19081 aaatgcgtcg cgggtgtcag caggcggaag aacagctcaa aatgctgatc gattatcaga
    19141 atgaatatcg caataacctc aacagcgata tgagtgccgg gatgaccagc aaccgctgga
    19201 tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat cgccagcaac
    19261 ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa aagaaacaac
    19321 gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa
    19381 accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg aggaaacctg
    19441 aatgattcgc ttagcgccct taattaccgc cgacgttgac accaccacat tgcctggcgg
    19501 caaagccagc gatgctgcac aagattttct cgcgttgttg agcgaagcat tagcaggcga
    19561 gacaactacc gacaaagcgg ccccccagtt gctggtggca acagataagc ccacgacaaa
    19621 aggcgagctg ctgatcagcg atattgtttc cgacgcgcaa caagcggatt tactgatccc
    19681 tgtggatgaa acactgcctg tcatcaacgt cgaacaatcc acatcaacac cgttaaccac
    19741 cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag acgaaaaagc
    19801 ggatgatctg aatgaagacg tcaccgcaag cctgagcgcc ctttttgcga tgttgccggg
    19861 ttttgacaat acgcccaaag tgactgatgt gccgtcaacc gtgttaccgg cagagaaacc
    19921 aacgctcttc acaaaactga cttctgcgca actcacaaca gcacagcctg atgatgcccc
    19981 cggcacgcca gctcagccat taacaccgct ggtagcagaa gcccagagta aagcggaagt
    20041 catcagcaca ccgtcaccgg tgaccgctgc cgccagcccg ctaattactc cacaccagac
    20101 acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt ctcacgaatg
    20161 gcaacaatca ttaagccagc atatttcgct attcacccgc caggggcaac aaagtgcaga
    20221 gttgcgtctg cacccgcagg atttaggtga agtgcaaatc tccctcaaag tggatgataa
    20281 ccaggcacaa atccagatgg tttcaccgca tcagcatgta cgcgccgcgc tggaagcagc
    20341 gcttccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc aaagcaacat
    20401 cagtggcgaa agctttagtg gtcagcagca ggccgcttcc caacaacagc aaagccaacg
    20461 cacagtaaac catgaacctc tggcggggga agacgacgat acgcttccgg ttcccgtctc
    20521 tatacaaggg cgcgtaacag gcaacagcgg cgttgatatt ttcgcctaac gtcagaggta
    20581 gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga taattagccg
    20641 ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat aagcaagaaa
    20701 agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc ctgtgccagc
    20761 gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc
    20821 gtcgtaccct ccccggtctt ctacgcgctg gataccttca cggtcaatct gggcgatgcg
    20881 gatcgcgtgc tttatatcgg cgtaaccctg cgcctgaaag atgaagctac ccgctcgcgg
    20941 ctgagtgagt atttgccgga agtccgtagc cgtttgctgt tactgttttc gcgtcaggat
    21001 gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat taaaaccacg
    21061 ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc
    21121 gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg aaattgatgc
    21181 gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta gcggcgaaag
    21241 tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac gtttgcaggc
    21301 gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt tcaacctgct
    21361 gcgtcgtagc ccggatataa ccgtcggggc catccgcatt cagccgtacc atgaatttgc
    21421 ccgcaacctg ccggtgccga ccaacctgaa ccttatccat ctgaaaccgc tgcgcggcac
    21481 tgggctggtg gtgttctcac cgagtctggt gtttatcgcc gtggataacc tgtttggcgg
    21541 cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccataccg aacagcgcgt
    21601 catcaaccga atgttgaaac tggcgcttga aggctatagc gacgcatgga aggcgattaa
    21661 tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg aaatttacca atatcaccac
    21721 ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca acctgaccgg
    21781 cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat tgttggttaa
    21841 cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca
    21901 ggtgcagcat tcacagctgg agctggtcgc caactttgcc gatatctcgc tacgcctgtc
    21961 gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg atcgcatcat
    22021 cgcccatgtt gacggcgtcc cggtgctgac cagtcagtac ggcactctca acggtcaata
    22081 tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg aggaacagcc
    22141 caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga cgatctgtgg
    22201 gctgaagcgt tgagcgaaca aaaatcaacc agcagcaaaa gcgctgccga cgcggtgttc
    22261 cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct gataatggat
    22321 attccggtca agctgaccgt cgagctgggc cgcacacgaa tgaccatcaa agagctgttg
    22381 cgtctgacgc aagggtccgt cgtggcgctg gacggtctgg cgggcgaacc actggatatt
    22441 ctgatcaacg gttatttaat tgcccagggc gaagtggtgg tcgttgccga taaatatggc
    22501 gtgcggatca ccgatatcat tactccgtct gagcgaatgc gccgcctgag ccgttagtga
    22561 tgaataacca cgctactgtg caatcttccg cgccggtttc tgctgcgcca ctgctgcagg
    22621 tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg ctggtaaaac
    22681 ggttgggatt tgtccctaaa cgcactggcg ttaacggtct gaaaattagc gccagtgctt
    22741 cgctgggcgc gcgtgaacgg gttgtggtgg tcgatgtgga agatgcacgg ctggtgctcg
    22801 gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca ccaacggaag
    22861 agataccgca gcccgatttt cagtcggtca tgaaaaactt gcttaagcgt agcgggagat
    22921 cctgatgcgt cgtttgttgt ctgtcgcccc tgtccttctc tggctgatta cgcccctcgc
    22981 cttcgcgcaa ctgccgggta tcaccagcca gccgctgcct ggcggtggac aaagctggtc
    23041 gctcccggtg cagacgctgg tgttcatcac ctcgttgacg tttattccgg caattttact
    23101 gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta acgcgctggg
    23161 aacaccctcc gcgccaccta accaggtatt gctggggttg gcgctgtttt tgaccttttt
    23221 tattatgtcg ccggtgatcg acaaaattta tgtcgatgcg taccagccat tcagcgaaga
    23281 gaaaatatca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct
    23341 gcgtcagacc cgtgaggcgg atttagggtt gtttgccaga ctggcgaata ctggcccgtt
    23401 gcagggacct gaagccgtgc cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt
    23461 gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta tcgacctggt
    23521 gattgccagc gtgttgatgg cattggggat gatgatggtc ccccccgcca ccattgctct
    23581 gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg tcggttcgct
    23641 ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca tgatgatggg
    23701 gactgaagcg atgaaagtcg cgctggcact ggctgccccg ctgttgctgg tggcgctggt
    23761 cacgggcctt atcatcagta ttttgcaggc cgccacgcag atcaacgaaa tgacgctgtc
    23821 gtttattcca aaaatcatcg ccgtatttat tgccattatt attgccggac cgtggatgct
    23881 caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata tcatcgggta
    23941 gcccgtacaa tgatgcaggt gacaagcgat caatggcttt cctggctaag tctgtacttc
    24001 tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag cgaacgcagc
    24061 gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgctat tgccccatca
    24121 ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc cgtgcagcag
    24181 atcctgatcg gcattgcgct tggtttcacc atgcaatttg cctttgccgc tgtgcgaacc
    24241 gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacgtttgt cgatccggcc
    24301 agccatctca acatgcccgt tctagcgcgt atcatggata tgctggcgtt actgctgttc
    24361 ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt tcacaccctg
    24421 ccgattggtg gcgaaccgtt gaacagcaat gcgtttctgg cactcaccaa agcagggagt
    24481 ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgct gctgaccctg
    24541 aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt tgttattgga
    24601 tttccattaa ctctgactgt cggtatctct ttaatggcgg cattaatgcc gttaattgcg
    24661 cctttttgcg aacatttatt cagtgagatg tttaatttgc tggctgatat tattagtgaa
    24721 ttgccattaa tataattccg taacgtttat catgttatcc taaggattat ccgaaaaata
    24781 atacctacga acatcttcca ggatactcct gcggcgaaat atttgtttta agctcactca
    24841 catatcgcaa catttacttt actttaagac aattccaggc aaattataca acactttacg
    24901 ggatagtaag tccgcctgaa aaatcgcgag agtggcgcat taggtgaccc atgttgttcc
    24961 gtttagtcat gatgaaatat tcaggtaagg ggaattatcg ttacgcattg agtgagggta
    25021 tgccatgtca acgattatta tggatttatg tagttacacc cgactaggtt taaccggata
    25081 tctgttgagt agaggggtta aaaaaagaga aatcaacgac attgaaaccg ttgatgacct
    25141 tgccatagct tgtgattcac agcgcccttc agtggtgttt attaatgagg actgtttcat
    25201 ccatgatgct tctaacagtc agcacatcaa gcacatcatt aatcaacatc ccaatacgtt
    25261 atttatcgtt tttatggcaa ttgccaatgt tcattttgat gaatatctat tggtcagaaa
    25321 aaatttattg ataagttcta aatcgattaa accggaatct ctcgacgata tccttggcga
    25381 tattctgaaa aaagagacaa cgataacctc ttttttaaat atgccgacgt tatcattgag
    25441 ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt cagggaacga ttcaaatctc
    25501 tgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata ttaaacgtaa
    25561 gatcaaaacg cataataaac aggttatcta ccatgtcgtc cgactgacgg ataatgtgac
    25621 taatggtatt tttgtcaaca tgcgctaaca cattctgact ggtggtttcc caccagtcag
    25681 gctgaataag attactctgc tttctccaca aagataccgt cctgatgccc tgcttcatta
    25741 aagaaccaga tgccgagcgg gtagtcttcc agcgaaacca ggtacattgt gccttcacta
    25801 aactcctcaa ctgccagtac cacgccagga cgacgcggac cgccatccgt tttgactgtt
    25861 acccgatcat tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt agaacaattt
    25921 cgtttttctg gcagcgccag gcgcgcgcga gtgctgattt tctcgacggt ctatacttaa
    25981 gagatgccag gcggacttaa cgactggcgg caacaacaga gtaacggttg cgaggaaaga
    26041 tgatgaaaac cgcaaaagag tacagcgata ccgcaaaacg tgaggtcagc gtcgatgtcg
    26101 atgccctgct ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc agccagaacg
    26161 attctgaaca cgttagcgtc gatggacgtg aatatcatac atggcgtgaa ttggcggatg
    26221 ccttcgaact ggatattcat gacttcagcg tctctgaagt gaatcgttga atgcacaata
    26281 aaaaaatccc gaccctgagg gggtcgggat gaaacttgct taagcaagaa gcacttaaaa
    26341 aattcgttac accaggaaat ctgatgtgtt catcacctta tccgcaattt ttttcgctga
    26401 caagaaaata ttcgcgatat gaatgattaa cgctacttat gaatagaaat gtgacgtgcg
    26461 tcaccccttg gtggctctgg cgtcaacggg atcgctcccg aaaaaatcat ctttgattat
    26521 ttgtgctgtt ttgagaatca tcctggtaag gggagaactg cgcgaccatg ttttcaattc
    26581 aacaaccact actggttttt agcgatcttg atggcaccct gctggacagt catagttatg
    26641 actggcaacc agctgccccc tggctcagcc gtttacgcga agcgaatgtt cccgtcattc
    26701 tctgtagcag taagacatca gcggaaatgt tgtacttgca aaaaacgttg gggctacaag
    26761 gtttaccgct gattgccgaa aatggcgcag tgatccagct tgctgagcaa tggcaggata
    26821 tagacggttt tccacgcatc atctcaggta ttagccatgg cgaaatcagc caggtattaa
    26881 atacgctacg cgagaaggaa cattttaaat tcacgacttt tgatgatgtc gacgatgcaa
    26941 ccatcgccga atggacggga ttaagccgta gccaggcggc gctgacgcag ctgcatgagg
    27001 cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat ggcacaattt accgctcgtc
    27061 tgaacgaact gggcttacag tttatgcagg gtgcgcgctt ctggcacgtc ctggatgcct
    27121 ctgccggaaa agatcaggcc gccaactgga ttatcgcgac ctatcaacaa ttatcaggca
    27181 aacgcccaac cacacttggc ctgggcgatg ggccaaacga tgcgccctta ctggaggtaa
    27241 tggattacgc ggtgattgtg aaagggctaa accgtgaagg ggtgcatctg catgatgagg
    27301 atccgacccg cgtctggcga acgcagcgtg aaggaccgga aggctggcgt gaagggctgg
    27361 accatttttt ctccgctcgt taagcgttat cgctcgcgca tacccgatta cgcccggcct
    27421 gtttagcgag ataaagccga cggtcagcca gtgactggag ttgttcaaaa tcataatcac
    27481 cggtttcctc gctgctacta acccccaacg aggcactgat gcgtatcgtc gtactcttag
    27541 caatcaacat ctctttttca tttaacttca ggcgaatacg ttctgcgact tccgcagcct
    27601 ccgtcagact cgcgcctggc agaatcacac aaaactcctc accaccgacc cgcccggcaa
    27661 cgtcctgcgc acgcaaggaa ctgctaatta atccggcagc atgagaaaga acacggtcgc
    27721 ccgcctgatg accaaagcgg tcattaatcg ctttaaaatg gtcaagatcg acctgaatga
    27781 cagaaaaagg atgttggtgc gtctgacaca atttagcgag cggacgggct ttttcgaaca
    27841 gtgcgccacg attatataaa cgggttaagg tgtcgtgcca cgcctgccac tgcaacgagc
    27901 tttgcagaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag atgagtaaca
    27961 tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactaccg aaatcgccgc
    28021 gcacgccttc gcttagcgta tggacgcgca ccagcacacc gtcgaaatgg tccagacgtt
    28081 cccagctaac atagcgactg tccatacgaa tgccgccccg cgtgtcatgt tccatcgcct
    28141 gcgccagcaa ggccagttca cgaggatcaa aaatattccc tgttggatgg tcaggattgg
    28201 aagaggtcaa aaatctcagc ttactgtcat agagctgata ctcaccatcg aggtttttat
    28261 cgatggcgtt tcttaaaaat tgctgcatag tacgcacagg aatactcatc cccagcacgc
    28321 cataccagta gttattactg tctaccggaa cactgacggt aacctgcggt tcagtattgc
    28381 tggcgtgttc cggttgcgaa gtaaaccagc gtaccgcgcg gtgacgattt tctcgttgcg
    28441 aatggccgat aaaccagggt tgggtgacat agccgtaata acgcgttgga acattgcgcg
    28501 taaacaaggt cggctgcgtc gaaacgtaaa atccggcacg cgagacatac atcgcctgtt
    28561 caaccatcga cgaggagttg tgcgccagtc gcagcaagta accaacttcc agtgcagcgg
    28621 taatttcatt gtcgaggctt tcattttcgc gagacaggag attcccctcg ctgactaatg
    28681 catccgacac accattaaat ggcagagtgc gccgtcggtt gagttcaatt tgccaggcgt
    28741 gctcgtcgcg atgctgttcg aactcggtta cagcattacg cagagaggtg aaatccagtg
    28801 gcgctacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg acgttatact
    28861 gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctgg ctggagatat
    28921 aggcatcttc cagcaccact acttcacgcc aggtgagcaa ggtggaaaaa agcaatacca
    28981 cgataaagca gagattaacg acatgaccag gccccaggcg gcgtgcgagt tttttcaacc
    29041 agctctggtt ttccattttt gtctcgtgct gcaccctgac gactcccaac ctgctttttt
    29101 atgattctgg cataacctgg cgatagcgta ccgcaaaaca gcgataattt tataatggtg
    29161 gcatgataag acgcgactat cattcattgc aggcattggc acaccaggcc ggatgtgatc
    29221 cggcctggtg ttaatgatac cctttcaaac ttcttccgca gaacgactcc cctcccccgg
    29281 aacaagctcg gtttcatgaa aagcttcccg cttgaccccg taaccgtcat accaacgaca
    29341 ctcaaccata ccgctggagt atccggtgac aatcatccgc gggccaccct ctttaaccgt
    29401 aacttcctca ctaaccataa agctcatact cgcctccttt tttcgagtga aacttgttca
    29461 ccttagttga agatggcgaa ttttgcataa tagcctgtgc gagtattaat gtgcctgacc
    29521 gcgcattttc gctacggctt tcactcccag caccacgatg ccgccgatga taaatccaag
    29581 aatcagattt aaaacagtcg gtaatatcat cgccaccact gcactttgct gcccggcgaa
    29641 atgttcaatg gcgtgatgca gcggcgcaat accatgtacc acaatcccgc cgccgacgag
    29701 gaacatcgcc agcgtgccga caatcgataa cgccttcatc aaccagggcg caatgatcaa
    29761 taacccttta cctaatgcct gcatcagcgc gctggatttt tccgccagcc aataccccag
    29821 gtcatcaatc ttaacgataa cccctaccag accgtagacg cccacggtca ctaccagcgc
    29881 gatgcctgaa agcaccagca cctgattaag caacggcgct tcggccacaa tccccagcgt
    29941 gatggcgacg atttcggcag acaatataaa atcggtacga atcgcccctt ttattttgtc
    30001 cttttcaaac ttcagcggat cctgcgccgc cagcttctcc agacgctgct ggctctgcgc
    30061 cggatcttct ttatgtttac gcgcctccag catatgcagc actttctcta ctccttcaaa
    30121 gcagagaaac gcgccaccaa tcatcaacag aggcgtaatc gcccacggga taaacgcact
    30181 gatgatcagc gccagcggca ccagaatcac tttattaatc agcgatcctt tcgccacgcc
    30241 ccagaccacg ggaagttccc ggttggcccg cacgcctgaa acttgttgcg cattgagcga
    30301 taagtcatcc cctaatacac cggcggtttt cttcgccgcc agtttgccca tcacggagat
    30361 atcgtccagt agtgtggcga tatcatcgag caacgtcagt aaactactcc cggccaaaag
    30421 cattccctct catttttatt gttgaagggt gtaagtatga agcaaaaacg gcagctctga
    30481 tacttgcttc acaggtcaac atttttttaa caattaagtg caattatatc tctcgccagc
    30541 agaatgattt tcacgtttac tatgaaccac ctttttattt ccaccgtgag ggattatgcg
    30601 tttccgccag ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca tttggggctc
    30661 aacctatttt gtcattcgga ttggcgtgga aagctggcct ccgttaatga tggcaggcgt
    30721 tcgattcctg gcagccggta ttttattgat ggcatttttg ctactgcgcg gacacaagct
    30781 ccccccgcta cgtccgctgc tcaatgccgc gctgattggc ctgttattgc tggctgtcgg
    30841 taatggcatg gtgacggttg ccgagcatca aaatgttcct tccggcatcg ccgccgttgt
    30901 ggttgcaacc gtgcccctct ttaccctgtg cttcagccgc ctgtttggca ttaaaacgcg
    30961 caaactggaa tgggtgggta tcgccattgg gcttgccgga atcatcatgc tcaatagcgg
    31021 tggaaattta agcggcaatc cgtggggcgc gattctgatt ttaatcggct cgattagctg
    31081 ggcgtttggc tcggtttatg gctcgcgcat taccttacct gtagggatga tggcgggtgc
    31141 tattgagatg ctggcggcag gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa
    31201 actgacggcg ctcccttccc tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg
    31261 ttcgattatc gccatcaacg cttatatgta tttaatccgt aatgtcagtc cggctctcgc
    31321 caccagctac gcctacgtta acccggtggt cgcggtcttg ctgggtacgg gactgggcgg
    31381 agagacgctg tcgaagattg aatggctggc gctcggcgta attgtcttcg cggtggtgct
    31441 ggtcacgttg ggaaaatatc tctttccggc aaaacccgta gttgcgccag ttattcagga
    31501 cgcatcaagc gagtaaatga atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc
    31561 agatccactc ttccagacgc tcgctaagcg cttcatccgt cagcttctcg cgcccacgta
    31621 acgcgcactc ccagacaatc aatacgcgcc agccgagttc ctgcaagcga ctgatatcgc
    31681 ggcgatcgcg ctcaacattt ttacctatct tctccagcca gaactcggtt cgagtcgcag
    31741 gcactttaaa cagatagcag tgatgatgat gccagaagca gccgtgggta aatatcacgc
    31801 agcgatattc atcaacgaca aaatccgggc gtccgggcag actggcgtcc tgaacgcgaa
    31861 acgccaggcc ctgcccggtt aacagactgg cgaggcgctt ctctatcgcc gtatcacgcg
    31921 tggcaatcgc gcgcatattt ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt
    31981 gcctcttgct gacgcaacgc caccgcctgt ttgatttttg gctcaagcag ttttgccacc
    32041 gcggcaaaga ccggcacgac caccgagtta ccgaactggc gataggcctg agtgtccgaa
    32101 accggaatgc ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg cgcgcattca
    32161 cgaggcgtta accgacgtgg acgatgttgc tgattctgcg gatcgtcaaa gtctttctca
    32221 cctttggcca tatcccagcc gcgatcgatt aaaatttccg cgccatcttt gtaataacgc
    32281 gcagacagcg tacgcgtgac gctttgcgga ttgttcggat aaaccattcc ataaccgaag
    32341 ccgttaccgc gcgcctgatg ttttttcgca tatcgataga ggtacttcca cagcaccggc
    32401 gtcaggatat atttcgcctc gaccatcggg tccagcagct gcgccagcgt cactcgctgc
    32461 gcagggaaac attcgctgat atcacgcagg gtaaaatcgg ctttcagatt cagatcgcga
    32521 cgaaaaccca ccagcacgat gcgttcacgg tgctgtggca gaaaatgttt gccgtcgatg
    32581 attttcggat cgtctggccc gttatcttct gcatcagcct cgtcatagcc cagttcgtcc
    32641 agcgtctgca tgatgatgcg gaacgtttta ccctggtcat ggcttttcag gtttttgacg
    32701 ttttcgagca caaacatcgc cggacgacgc gagtcgataa tgcgcaccac atcaaaaaac
    32761 agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat
    32821 acgccagcca gcgaaaatgg ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga
    32881 atgtgttgac gaatatgttc cgccgccgcc tcatcactca cgccttcttt atggctgagg
    32941 gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg ggtcgcaata atgattggct
    33001 ttataagtgc gtaccgcatg tttgttccat tcgctggtaa acacgcactg tccgccaatc
    33061 gattcaaaac cgcgacggat gccgccaatt ccggcaaata gatcgataaa gcgaaacgca
    33121 taatgcggat gatgtgccgg tggtttgggt aacaacgttt gcagatgggc gaactctttc
    33181 tcacttaaac ggtgccatgc cgagtcattc gccagcgcac gttttaaaat tgccgcgctc
    33241 cagtgattct cacccacacc attaagctgc gccaccagcg ttttaacatc ataaatttgc
    33301 agcagtttct ccagcattgc ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat
    33361 atattttcct gcatagattt aaccggccat ctgagatggc cgacaggtta acataattac
    33421 agccatttag ggccacgcgc ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg
    33481 acaccttcat tctcccccgc cagttcggca ctgagcttcg ccataaactc gactaaaaaa
    33541 tgcgcgttgt gctgcgccag ctgcttgcct cttgcggttt gcatggtttg cggtagcttc
    33601 agcaacttag tctggaaatg atccagctcg tagcgtttat catcaagcgg gcgatgctgt
    33661 gcgaacggat cttcaccatc aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg
    33721 cgcgccaggc caatcgcccc cagggcttcc agccgatccg catcctggac gattttagct
    33781 tccgttgtta agggggcgat ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg
    33841 gcctcgattt tctccgccgg aaattgctcg aactcttcac ggagcaggcg acgcgtctct
    33901 tctgccgcca ggattgaaga acgctgcctt tgcggatgat ttttcgccag gctcacaata
    33961 tcgtgaaaat aacaggcggt taaaatcacc agcatatcga catcgtcatc tgccgccagc
    34021 ttctgtgccg tagcccagac gcggcgaaaa tgacacacgt catgggccgc gccctggtgc
    34081 tgatgatggt ttttcaacca gttttcgaac tgtgcctgcc agtgttgtaa gtccatacgc
    34141 gctccgttgc caaagagtcg ccaccttagc agtttttatt catagcgtct gctacgtgcg
    34201 ggacgaggtc gggtgtacca ggcaatgctg cctaacaatg ccccgaccag agccataaat
    34261 aaaaatccga ccagtgcccc taaccacttt cccgcggtag agcctaaatc gctttcaaat
    34321 cccgacaccg gggcatcggg gatccggctc atcacccaga tgtaacgcgc cattgcccag
    34381 acgaaatagc cacaccatgt atagaaagcc cacaaagcca gtttgccacc agggctgcga
    34441 gagagtctct cttccatctt tgtcgaaccg tattccattt tgagataacc cggaaaattc
    34501 agacttagca ctattatgtg ataaaagtca catttttcca cattggatga gccagtcaag
    34561 actcactcat tttttgaaca aatagataat ttttgcgcaa cggcagtgaa tacaggaaaa
    34621 atcgttttga gcgattttat ttatctttga gatcggcttt attaattcat ttaatcaata
    34681 tattagcact gaatacaatt ataccttttt atttttctgt gtcatgatgc ctccgttatt
    34741 agccttttat cgtcttgttt atattttttg ggccggcatg atgccggctt ttttttatgc
    34801 cttcattaat gtgcgcgtga tcacaccagc cgtttggcgc aacaatcatt gataccccc
//
LOCUS       DAZFWP020000038        33766 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFWP020000038 DAZFWP020000000
VERSION     DAZFWP020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.038"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..329
                     /locus_tag="ACHK0Z_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             81..329
                     /locus_tag="ACHK0Z_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4501712.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHK0Z_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             307..1437
                     /locus_tag="ACHK0Z_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4501713.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHK0Z_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHK0Z_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU4501714.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHK0Z_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHK0Z_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHK0Z_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHK0Z_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU4501715.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHK0Z_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHK0Z_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU4501716.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHK0Z_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHK0Z_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU4501717.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHK0Z_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHK0Z_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU4501718.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHK0Z_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHK0Z_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU4501719.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5872..7125
                     /gene="kch"
                     /locus_tag="ACHK0Z_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             5872..7125
                     /gene="kch"
                     /locus_tag="ACHK0Z_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU4501720.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7180..7353)
                     /gene="yciY"
                     /locus_tag="ACHK0Z_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             complement(7180..7353)
                     /gene="yciY"
                     /locus_tag="ACHK0Z_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU4501721.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7496..8956
                     /gene="cls"
                     /locus_tag="ACHK0Z_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             7496..8956
                     /gene="cls"
                     /locus_tag="ACHK0Z_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU4501722.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8991..9320
                     /gene="yciU"
                     /locus_tag="ACHK0Z_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             8991..9320
                     /gene="yciU"
                     /locus_tag="ACHK0Z_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU4501723.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9373..10377)
                     /gene="oppF"
                     /locus_tag="ACHK0Z_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             complement(9373..10377)
                     /gene="oppF"
                     /locus_tag="ACHK0Z_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU4501724.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10374..11387)
                     /gene="oppD"
                     /locus_tag="ACHK0Z_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             complement(10374..11387)
                     /gene="oppD"
                     /locus_tag="ACHK0Z_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU4501725.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11399..12307)
                     /gene="oppC"
                     /locus_tag="ACHK0Z_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             complement(11399..12307)
                     /gene="oppC"
                     /locus_tag="ACHK0Z_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU4501726.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12322..13242)
                     /gene="oppB"
                     /locus_tag="ACHK0Z_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             complement(12322..13242)
                     /gene="oppB"
                     /locus_tag="ACHK0Z_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU4501727.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13328..14959)
                     /gene="oppA"
                     /locus_tag="ACHK0Z_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             complement(13328..14959)
                     /gene="oppA"
                     /locus_tag="ACHK0Z_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU4501728.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEARRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15243..15539
                     /locus_tag="ACHK0Z_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             15243..15539
                     /locus_tag="ACHK0Z_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501729.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(15697..16344)
                     /gene="ychE"
                     /locus_tag="ACHK0Z_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             complement(15697..16344)
                     /gene="ychE"
                     /locus_tag="ACHK0Z_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU4501730.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(16375..16428)
                     /gene="ychE"
                     /locus_tag="ACHK0Z_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             complement(16375..16428)
                     /gene="ychE"
                     /locus_tag="ACHK0Z_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU4501731.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16821..19496
                     /gene="adhE"
                     /locus_tag="ACHK0Z_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /gene_synonym="adhC"
     CDS             16821..19496
                     /gene="adhE"
                     /locus_tag="ACHK0Z_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU4501732.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(19798..20415)
                     /gene="tdk"
                     /locus_tag="ACHK0Z_RS08475"
                     /old_locus_tag="pgaptmp_001695"
     CDS             complement(19798..20415)
                     /gene="tdk"
                     /locus_tag="ACHK0Z_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU4501733.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            21020..21433
                     /gene="hns"
                     /locus_tag="ACHK0Z_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             21020..21433
                     /gene="hns"
                     /locus_tag="ACHK0Z_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU4501734.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(21577..22485)
                     /gene="galU"
                     /locus_tag="ACHK0Z_RS08485"
                     /old_locus_tag="pgaptmp_001697"
     CDS             complement(21577..22485)
                     /gene="galU"
                     /locus_tag="ACHK0Z_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU4501735.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(22687..23700)
                     /gene="rssB"
                     /locus_tag="ACHK0Z_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             complement(22687..23700)
                     /gene="rssB"
                     /locus_tag="ACHK0Z_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU4501736.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(23792..24697)
                     /gene="rssA"
                     /locus_tag="ACHK0Z_RS08495"
                     /old_locus_tag="pgaptmp_001699"
     CDS             complement(23792..24697)
                     /gene="rssA"
                     /locus_tag="ACHK0Z_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU4501737.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            24810..25268
                     /gene="ychJ"
                     /locus_tag="ACHK0Z_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             24810..25268
                     /gene="ychJ"
                     /locus_tag="ACHK0Z_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU4501738.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            25318..26160
                     /gene="purU"
                     /locus_tag="ACHK0Z_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             25318..26160
                     /gene="purU"
                     /locus_tag="ACHK0Z_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU4501739.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            26320..26404
                     /locus_tag="ACHK0Z_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     tRNA            26320..26404
                     /locus_tag="ACHK0Z_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26354..26356,aa:Tyr,seq:gta)
     gene            26452..26583
                     /locus_tag="ACHK0Z_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     ncRNA           26452..26583
                     /ncRNA_class="other"
                     /locus_tag="ACHK0Z_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            26630..26761
                     /locus_tag="ACHK0Z_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     ncRNA           26630..26761
                     /ncRNA_class="other"
                     /locus_tag="ACHK0Z_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(26945..27622)
                     /gene="narI"
                     /locus_tag="ACHK0Z_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             complement(26945..27622)
                     /gene="narI"
                     /locus_tag="ACHK0Z_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4501740.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(27622..28332)
                     /gene="narJ"
                     /locus_tag="ACHK0Z_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             complement(27622..28332)
                     /gene="narJ"
                     /locus_tag="ACHK0Z_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4501741.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASENLSKEDAHALG
                     IFLRDLTALDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(28329..29867)
                     /gene="narH"
                     /locus_tag="ACHK0Z_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     CDS             complement(28329..29867)
                     /gene="narH"
                     /locus_tag="ACHK0Z_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4501742.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKAQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(29864..33607)
                     /gene="narG"
                     /locus_tag="ACHK0Z_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     CDS             complement(29864..33607)
                     /gene="narG"
                     /locus_tag="ACHK0Z_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU4501743.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         8602 a         8538 c         8022 g         8604 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcttggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtcaggcgt gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccgccggtgc
     4801 ttcttttggt ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa atcctttgat
     5761 tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag
     5821 aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac
     5881 tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca cgacattctc
     5941 gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc
     6001 aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc cttgctggca
     6061 aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc
     6121 cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttacacc
     6181 ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac gctggtgttt
     6241 ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca
     6301 tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa
     6361 ggttttaatc cgcgaataga aagtttgatg acggcgttct atttttcgat agaaaccatg
     6421 tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt
     6481 tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat ttttggtccg
     6541 cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa
     6601 gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct gcaactgaat
     6661 caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt
     6721 gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta
     6781 ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga taacgatgca
     6841 gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc
     6901 gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc
     6961 tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat
     7021 aatgatatgc tcgtttcaat gttgttgaac tcaggtcatg gtattttcag cgataacgat
     7081 gaacaagaaa cgaaagctga cagtaaagaa tcggcgcaaa aatagagttt aaagacggaa
     7141 gagtaaactt attgggcacc gcaatggtgc ccagtacatt tagatattgt agatcgcaaa
     7201 caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt
     7261 tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg
     7321 ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca ataacagaat
     7381 ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag
     7441 atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac
     7501 aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg
     7561 cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt
     7621 gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca
     7681 tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct
     7741 taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc
     7801 attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca
     7861 actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg
     7921 ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc
     7981 tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc tcgactccgc
     8041 cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga
     8101 agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg
     8161 ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat
     8221 ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc
     8281 gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat
     8341 tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca
     8401 ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat
     8461 tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgaccacgcc
     8521 ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc agcgcggggt
     8581 ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg
     8641 cgcattcttt acggaacttc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt
     8701 actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct
     8761 tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt
     8821 tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc
     8881 ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt
     8941 cagtccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact atggatatgg
     9001 atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg
     9061 aactggctgc cgacaatctc gatccagctg atgttctgct gttcaatctt cagtttgaag
     9121 agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat gttgatttcg
     9181 atttgaaccc agactttttt gccgaagtgg tgattggtct ggcggacagt gaagatggtg
     9241 aaatcaacga tgttttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc
     9301 atatcatctg gcgggaataa ataatcgctt ttagcgtgaa aaagggctga caactgtcag
     9361 cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc
     9421 ctccagaaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac gggtacggaa
     9481 aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt
     9541 tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta
     9601 aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag
     9661 atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcgataaa
     9721 aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg
     9781 aattgacacg tccagcgccg aaaccggctc atcgcagata atcagcttcg gttcaagaat
     9841 aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat gcggatagcg
     9901 gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac
     9961 ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat
    10021 ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg
    10081 aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc aggcaacatg
    10141 accgtcggtc gctttaacca agccaatgat ggcgcgagca aaggtggact taccgcatcc
    10201 cgattcccct accacaccta atgtttcccc ttcatacagg cgaagagtga caccatcgac
    10261 ggctttgagc gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg
    10321 cactttaaga tcggcgattt caaggaggac ttttcttcct tcagttacag cattcataac
    10381 agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc ttccagcggc
    10441 ggtgcgctac tacaaatttc catcgcatgt ggacaacgtg gctggaacgg gcaacctttc
    10501 ggtaatcgca gcaagtttgg cggattaccc gggatggtca acattgtttc accttccgca
    10561 tcaagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag
    10621 acatcgcgcg cattgccata ttccatcgtg cgcccggcgt acattaccag cactttgtcg
    10681 cagatccccg ccaccacgcc aagatcgtgg gtgatcataa tgatggcggt attaaattcc
    10741 cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgcagta
    10801 gtgggttcat ccgcaatcag tagcttaggt cgacatagca atgccatcgc aatcatgact
    10861 cgctgacgca tgccgccaga aaattcgtgc gggtacattt tcatgcgttt acgcgcttcc
    10921 ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg
    10981 ttcttatgca gcatcagcac ttccattaac tgctcaccga cgcgcatata gggattcaac
    11041 gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca
    11101 tgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg
    11161 ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca
    11221 cctacaatac ccagcgtttc tccggcacgt agggaaaaat tcaaatcatt gaccgccgtg
    11281 acgtcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtgcgtca
    11341 gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt
    11401 aacgatcttt cgggtcgagg gcatcacgca agccatcgcc gataaagttg aaacaaaaca
    11461 gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt
    11521 tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca
    11581 ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca
    11641 ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac
    11701 cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt tgcccacgca
    11761 caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc agcaggatgt
    11821 tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag gagttgagga
    11881 tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac
    11941 cataaagtgt cccgacgacc acagccacca gtgccgcagc aacaccgacc atgagtgaga
    12001 tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt
    12061 agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg
    12121 caaactggga aagcatcggt gccaggatta caaataacgc gatcagcacc agcactatca
    12181 gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc cacaagctgc
    12241 gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta
    12301 acatcatcgc gagctccagt atcagtaacg aattttcggg tcgataaccg catatagcac
    12361 atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac
    12421 taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc
    12481 ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata
    12541 agagagcacg ggtaacagag caggttttaa tgcgtgacgt aaaatgatcc gccgcatagg
    12601 taaccctttc gcccgggcag tacgaataaa attggagtgt aatacttcaa tcatagagcc
    12661 acgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat
    12721 catgaattta agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc
    12781 aaatatcatg actaataatg gtgcaaccac aaaactgggg ataacaaccc cggtcattgc
    12841 cagccccatc acggtatagt cccatttggt gttttgtttt aatgcggcaa taacgccagc
    12901 actaacaccc agtattaccg caaggaaaaa tgctgcggct cccagtttgg cagaaacggg
    12961 aaaactggat gcaaccaagt cattgaccga ataatcttta tatttaaacg atggaccgaa
    13021 atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag
    13081 atgatatttc gcttcgatat tggccatcac ttctggcggt aaagtacgtt cgccggtaaa
    13141 agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gttataagaa taaatagcgt
    13201 cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct
    13261 gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt
    13321 attgccatta gtgcttcaca atgtacatat tccgggtata ggtattatcc agcggatctt
    13381 tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag
    13441 gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgcc
    13501 gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat
    13561 aatgcgcggt attcatcgag ctgttcgaaa gcatggtgtt caggaaggaa gttggttcgt
    13621 tgtagtcagc acaccagcct gcacgggcca catcaaaagt accctggtga cgggtgtcga
    13681 ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca
    13741 atgaagaggc agcaatcgcc agctttttat gcagatcgga ggtgttatac aacaggttga
    13801 tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt
    13861 tacgtttttc ctggctccag ccaaaccatt ccggctgagt caattttgcg ccatcagtat
    13921 acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga
    13981 tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt
    14041 tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg
    14101 ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttataa gtcatgtcga
    14161 tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta
    14221 cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc
    14281 gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct
    14341 gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt
    14401 gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat
    14461 catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa
    14521 taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg ttcggatcaa
    14581 cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcca tcagaccatt
    14641 tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag
    14701 cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc
    14761 ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac tgaacttctg
    14821 aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag
    14881 ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct actaaacttc
    14941 tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca
    15001 gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta
    15061 ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca gacgatcacc
    15121 ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt
    15181 taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc ggaaagtacc
    15241 aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc tcttttattg
    15301 accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca attcaatcaa
    15361 atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt gaaatgcaca
    15421 ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt ggaattacta
    15481 atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa ataacatgat
    15541 ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga agaatatttt
    15601 tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa tgacgaagta
    15661 aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc cggggaaaat
    15721 ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata gccccataat
    15781 acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg gtgccatgcg
    15841 gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac caaacagata
    15901 gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc ctggccccgc
    15961 catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg tttctgattt
    16021 ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca tcgctatcgt
    16081 caccaccagg atgcccccgg cgatacggaa cgaatcaatt gatataccaa aaagttgcag
    16141 aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca ggttggctgt
    16201 aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga taaagacggg
    16261 aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga agtaaacggg
    16321 aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat aatgttagcc
    16381 ataaataagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt ttcacctcct
    16441 aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac atgctaatgt
    16501 agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa agcgatgctg
    16561 aaaggtgtca gctttgcaaa aatttgattt ggatcacgta atcagtaccc agaagtgagt
    16621 aatcttgctt acgccacctg gaagtgacgc attagagata ataactctaa tgtttaaact
    16681 cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca taggttaagc
    16741 aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact aaaaaagttt
    16801 aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac gcactcgtag
    16861 agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa gtagacaaaa
    16921 tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg aaaatggccg
    16981 ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac tttgcttctg
    17041 aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct gaagacgaca
    17101 cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc gttccgacca
    17161 ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc cgtaacgcca
    17221 ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct gatatcgttc
    17281 tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc gatcaacctt
    17341 ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc ctcgcaactg
    17401 gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc ggtgtaggcg
    17461 cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca gttgcatctg
    17521 tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag tctgttgttg
    17581 ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc ggctatctgt
    17641 tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt gcgctgaacg
    17701 cggctatcgt tggtcagcca gcctataaaa tagctgaact ggcaggcttc tctgtaccag
    17761 aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa ccgttcgcac
    17821 atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa gacgcggtag
    17881 aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc ctgtacactg
    17941 accaggataa ccaaccggct cgcgtttctt acttcggtca aaaaatgaaa acggctcgta
    18001 tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac ttcaaactcg
    18061 caccttccct gactctgggt tgtggttctt ggggtggtaa ctccatctct gaaaacgttg
    18121 gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac atgttgtggc
    18181 acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg ctggatgaag
    18241 tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg ttcaacaatg
    18301 gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact gaagtcttct
    18361 tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa ctggcaaact
    18421 ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac gccgcgaaga
    18481 tcatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg ctgcgcttta
    18541 tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg aaaatgatcg
    18601 ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt gtaactgacg
    18661 acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat atggcgattg
    18721 tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt ggtctggacg
    18781 cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc tctgatggtc
    18841 aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac cacgaagggt
    18901 ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc gactatcgcg ggtatcgcgt
    18961 ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt tcccagttcc
    19021 atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc tacaatgcga
    19081 acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag gctcgccgtc
    19141 gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt actgctgcta
    19201 agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt attccgaaat
    19261 ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat aaactgtctg
    19321 aagatgcgtt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg atctccgagc
    19381 tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt gaaactgcag
    19441 cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc gcttaatcag
    19501 tagcgctgtc tggcaatata aacggcccct tctggggccg tttttttgtt tacccaaagc
    19561 aacttttcca taaaccgaca gcattagcct tcatcatatt tgcgacgatg tataacgcct
    19621 aaacacaggg atattgtact ttataggtca caagtcaacg tcggtgctta agagccctgt
    19681 gaggcgtata gcggcgttaa aaaactgccg agaagggtat atagcccgga agaagtgcgt
    19741 aaaacgaact gacaggataa aagtgccctg ctcaccctgt cagtaaagaa attcttatta
    19801 atcgtggcga tgcctttcct gaatagccgt taatgagccg acttgtaacg cctctttata
    19861 gtgtttacgg catacagaaa cgtatcgttc attaccacca attaccacct gctcaccttc
    19921 gttataaggt ctgcctgctt gatcaagacg cagcaccatg cttgctttac ggccacaaaa
    19981 acagatggtt tttaattcaa ccagtttgtc ggaccatgcc agtaagtatt ggctgccaat
    20041 aaataattca cctcgaaaat cggtacgtaa accataacaa agtacgggta tatcgagttg
    20101 atcgacaacc tccgataatt catatacttg ttgtctggtt aaaaactggc attcatcaac
    20161 cagtacgcaa tgaattgcct gctgttcatg ttccgcacga atctcatcaa ataatgatga
    20221 attttggtta aataattttg caggcgatga caaacctata cgcgaactga ctttcccggc
    20281 accaaagcga tcatcaattt ctgccgtata tacgacagtg cgcatgccgc gttcctggta
    20341 attgtatgaa gattgcaaca atgctgtaga cttacccgca ttcattgcgg aatagtagaa
    20401 atatagctgt gccatcagcc acaggccctc aatgatatga aataaagttg gctggagttt
    20461 atcataattc gtcagttttt cagctcaaca gtatgcacag aagtgggtat atgcgttctc
    20521 ccttacgaag ccttgcataa tccttctgag ctatcattac aactgcctcg cttgttataa
    20581 gcgggtaaat gactgctggt aactattcac aatctttaac ctgttgcgca agtaatagcc
    20641 ctctgttgac ctccaggaga tagtgcaata ctaagtccat gctcttattg cgactgttct
    20701 acttttcatc attcgcttaa tagggaattc tcgtaaacac aactaataca gaagactgaa
    20761 aggtcgtcag cctacgataa tctccccata aaatgtgaca tgaatcagga agttttaacc
    20821 tcacgtgctg cgaaatcatc ggtgtaaata gggctatatg ccgcgtcttt tctggctaat
    20881 tttatgaaaa gatatttatt ggcggcacaa aataaagaac aattttgaat tccttacatt
    20941 cctggctatt gcacaactga atttaaggct ctattattac ctcaacaaac caccccaata
    21001 taagtttgag attactacaa tgagcgaagc acttaaaatt ctgaacaaca tccgtactct
    21061 tcgtgcgcag gcaagagaat gtacacttga aacgctggaa gaaatgctgg aaaaattaga
    21121 agttgttgtt aacgaacgtc gcgaagaaga aagcgcggct gctgctgaag ttgaagagcg
    21181 cactcgtaaa ctgcagcaat atcgcgaaat gctgatcgct gacggtattg acccgaacga
    21241 actgctgaat agccttgctg ccgttaaatc tggcaccaaa gctaaacgtg ctcagcgtcc
    21301 ggcaaaatat agctacgttg acgaaaacgg cgaaactaaa acctggactg gccaaggccg
    21361 tactccagct gtaatcaaaa aagcaatgga tgagcaaggt aaatccctcg acgatttcct
    21421 gatcaagcaa taatcttttg tagattgcac ttgcttaaaa tcccgccagc ggcgggattt
    21481 tttattgtcc ggtttaagac aatttaataa gaataagcta taaaaaaacg gcgtcgattg
    21541 ctcaacgccg tttcgtggat aacaccgata cggatgttac ttcttaatgc ccatctcttc
    21601 ttcaagccag gctttaaatt ccgtgccaag ggtgttatga cgaataccgt attcaacgaa
    21661 ggcctgcatg taacctaatt tattaccgca gtcatggctc ttccctttca tatgataggc
    21721 ttccaccgtt tctttttcga tcagcatatc aattgcgtcg gtgagctgaa tttcatcacc
    21781 agctcccgga ggggtttttg ccagcaacgg ccaaatatcc gcgctaagta cgtaacgacc
    21841 cacaatagcg agattagacg gcgcaacatc cgctttcggt ttttctacca caccaaccat
    21901 cggtacgctt tcacccggcg ctaattcaac gcctttgcaa tccacaacgc catatgcggt
    21961 cacatcagca accggttcaa ccatgatctg gctatgaccc gtttcatcaa agcggcggat
    22021 catctctgcc aggttatcct gtgacaaatc ggattcatat tcatccagaa taacatcagg
    22081 caaaataaca gctaccggtt catcacccac taccgggtga gcacacaata ccgcgtgtcc
    22141 caggcctttc gccagcccct gacgaacttg cataatagtt acgtgcggcg gacaaataga
    22201 ctgcacttca tcaagcagtt gacgttttac acgtttttcc agcattgctt ccagttcaaa
    22261 actggtatca aagtggtttt caatagagtt tttagatgag tgagtaacca gcacaatttc
    22321 agtaatgcca gccgcaatac attcattcac gacgtattga attaatggct tatcgacaag
    22381 tggcagcatc tctttcggga tggctttcgt cgccggcaac atcctggttc ctaatcccgc
    22441 aacggggata acggcttttt tgactttcgt attaatggca gccatttaaa ttctcctgga
    22501 ctgttcgtgt tttgaacgtg ttcatatttc tgtatcgcat cccagtatat cagcaccccg
    22561 aaggggctaa gttctgaaaa ggaggcgagt tagcataatt aagacaatta cttatacgaa
    22621 tggcgacgat agtaccacca gccgacatta gcaggtaatg caaatttagc ccgcgttatc
    22681 gtttgctcat tctgcagaca acatcaagcg cagtcgacca ccggttcccc atatttggca
    22741 ttgccaggca tcgcatcgct ggctcaattg attcaaataa gcgttaccta aagtgcctaa
    22801 cggaacacca ttactgattt gcacctggtg ttcgccggta tttaacgtcg cattcagacc
    22861 cgcagaaacc agaatgagat ttttcagttc gcgatgataa tagccaacta atagcggaaa
    22921 ctgccccggc agattggcct gacggagtaa atggtttacc tgcttcaata acgcgcccaa
    22981 ctctggcaac cgttgatttt ggtgtgcaag ctgttcctgt aataatccgt taaacaatgc
    23041 gcgtaataac aaggcagcaa gtacgccatt atgtccagct cgagtgacat caaggcaata
    23101 aaatgccaga tcgttttccg aaagtgcggc aatatcaagc accaggccgg gtttgtccgc
    23161 ggcaaccaat tgacgataat taacccggca atgggaaatc acctgctgaa ccggcggttg
    23221 tagttcctgt aataatttcg ccgctgcggc agggttatca accattgcat cccagtcgcg
    23281 aaaaagcctt tcctcttcct caacgcgcga attaaacatg ctgggataga gacaggcaaa
    23341 aaccatctcg cgcaagcgat tcagatcttt aactggtttc agcaaaacat cttcaacgcc
    23401 cagacgtaac gctttggcaa tatctgccat attttcagtg gcagatatca ccagaactgg
    23461 ggtctggtcg cctctgttac gtatatgctc cagcagttta agcccgttca ttcgtggcat
    23521 cgcgatatca catatcatca ggtctggagt gaaacctccc agcaactcaa gggcatccac
    23581 cccatcagcc gccagtaccg ttgtcgctcc caatgaggaa aaccatgaat ccagaagcga
    23641 gcgaaatacc tgctcatctt caacaatgag aatctgtttt ccgaccaatg gctgcgtcat
    23701 gttctctccc ctgactggct ttactcaata gtggcatgct attgccacct gcgcctgtca
    23761 gaattaactt aagtgtagat aaaaattctg gtcaaatgtt ggtgcgtacc aacggcaaaa
    23821 gttcgtccat tttcctttcc actgccagct gtccggccgc aatggcagcg tgcgcgcgat
    23881 ggaaatcaag cgtagatatt tgcgggcaaa caggttgaat cagaatatcg ggcggatcac
    23941 ctgccatgcg gttcctttta aggcggttct ccagcacctg gatagaagtg gtcataatct
    24001 ctgttgccgt tggcgctgtc accgcccgac gtgtcgttat gctgcctaac ctttctttca
    24061 gacgcgcatg ccacggcaga gaatcaccat tctcgctatt ttcttcactg acattaaagg
    24121 agagcaaatc ttgttgcatc aaatgagcat cgtgctgcag gtcaaccgct atcacaatat
    24181 cagcccccaa tgcacgcgtg agggaaatag gaattgggtt aacgactgct ccatcaacca
    24241 gccagtagcc gttatgtgca acaggtgcca tgagtcctgg aatactgcat gatgcgcgaa
    24301 tagcaagatg gagatcgcct tcagtaaacc ataattcacg tcccgtactt aaattggtgg
    24361 caacagccgc aaagcgacgg gaacaatttt cgatctctgt ttccggcatt atttcgcggt
    24421 attgattgaa gacacgctcg ccgcgcagta acccaccgcg ctgccaggag agatccatca
    24481 ggcgtaaaac atcccaataa ctgaaagagg tcacccaatc ttccagcgca gataatcgat
    24541 cgcatgcata ggcagcgccc accagcgaac caattgaaca tcctgcaacg atatcaattt
    24601 caatacccac tttttttagc gcattaataa cgccaatatg cgaccaacct ctcgccgcgc
    24661 cagatcccag cgccagccct atttttatct ttctcattat tcccgcaaga ttcccctgga
    24721 atgcaatcgt agccacatcg ctactgctcg gttaacatag tgctactcag gcggttaata
    24781 cgccgttatt gtttccaggg agattatttg tgtctcagct ttgtccctgt ggtagtgctg
    24841 tcgagtatag cctatgttgc cacccttatg tgtctggtga aaaggttgca cctgatccag
    24901 aacatctcat gcgttcgcgt tactgcgctt ttgtgatgca agacgcagat tatttaataa
    24961 agacctggca tccctcttgt ggagcagcgg cgttacgtgc cgaattgatg gccggatttg
    25021 cgcatactga atggttggga ttgaccgtat ttgaacattg ttggcaggac gccgacaata
    25081 tcggatttgt cagttttgtt gctcgattta ccgaaggtgg taaaacaggt gcaataattg
    25141 aacgttcgcg atttttgaaa gaaaacggtc aatggtacta tattgacggt acacgtccgc
    25201 agtttggtcg caacgatccc tgcccttgtg gttcaggtaa aaaatttaaa aagtgctgcg
    25261 gccaataatg gttgacggta cggtttagca aacactctca acaaggtttt tccagcaatg
    25321 cattcactcc aacgtaaagt tctgcgtact atttgtccgg accaaaaagg tctgatcgca
    25381 cgtattacca atatttgcta caagcacgag ttaaatatcg tacagaacaa tgaatttgtt
    25441 gatcaccgta ccgggcgctt ttttatgcgc acggaactgg aagggatttt taatgattcc
    25501 accctgctgg cggatctcga tagcgcattg ccagaaggct ccgtgcgtga gctgaatcct
    25561 gccggtcgtc gccggatagt gattctggtc actaaagaag cgcattgcct tggcgatttg
    25621 ttgatgaaag ccaattacgg cggcctggat gtcgaaatcg cggcagttat tggtaaccac
    25681 gatactttac gttctctggt tgagcgtttt gatattccgt ttgagctggt aagccatgaa
    25741 gggttaagcc gcaacgagca cgatcaaaag atggcggacg ccattgatgc ttatcaacct
    25801 gactatgtgg tgctggcgaa gtatatgcgg gtattaacgc cggaatttgt ggcacgcttc
    25861 ccgaataaaa tcatcaatat tcaccattca ttcctgcctg cgtttatcgg cgcacgtcct
    25921 tatcaccagg cctatgaacg tggtgtgaag attattggcg caaccgctca ctatgtgaat
    25981 gacaatctgg acgaaggccc aatcatcatg caggacgtta ttcatgtcga tcatacctac
    26041 acagctgaag atatgatgcg cgcaggtcgt gacgtcgaga aaaacgtctt aagtcgtgcg
    26101 ctatacaaag tactggcaca gcgcgtcttt gtttacggta atcggacgat tattctttaa
    26161 tcgccagcaa aaataactgg ttacctttaa tccgttacgg atgaaaatta cgcaaccagt
    26221 tcatttttct caacgtaaca ctttacagcg gcgcgtcatt tgatatgatg cgccccgctt
    26281 cccgataagg gagcaggcca gtaaaaagca ttaccccgtg gtggggttcc cgagcggcca
    26341 aagggagcag actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc ttcccccacc
    26401 accatcactt ttcaaaagtc cgtaaactct caaacgaatc cgcaattaaa tattctgccc
    26461 aagcggggaa ggatgagaag cttcgaccaa ggttcgactt gagcgccagc gagagagcgt
    26521 tgccgcaggc aacgacccga agggcgaagc gcgcagcgct gagtaatcct tcccccacca
    26581 ccatcacttt caaaagtccg tgaactctca aacgaatccg caattaaata ttctgcccaa
    26641 gcggggaagg atgagaagct tcgaccaagg ttcgacttga gcaccagcga gagagcgttg
    26701 ccgcaggcaa cgacccgaag ggcgaagcgc gcagcgctga gtaatccttc ccccaccacc
    26761 atcgcttcct taaataatca aaacctgtta ctccgagcta attttgcccg atggcaccac
    26821 gtttaccggg cctacaatat cctcccctgt aggtcggata agcgaagcgc atccgacaac
    26881 tcaccccgca ccgtgcccat aaaaaaaccc cgccgaagca gggtctatgt gaactaaaat
    26941 tcgcttagtg acgagcgcgc accagctggt acttacgtgt cagatactcc accggtacgc
    27001 tccagatgtg taccagacgc gagaacggga acagcaggaa taacgtcatc cccagcacca
    27061 ggtgcagacg gaagataaac gccacaccat cgaggtgttg agaagcgcca ccgtggaagg
    27121 tcaccaccga ctgcgcccag ccaaccagtt tcatcatctc gctaccgtcc atatgctgag
    27181 cggagaacgg aatggtcagc aggcccagcg cgcactggat aacgagcagc gacaggatca
    27241 ggatatccgc tccagtagtg gttgcacgca cgcgtgggct gaacagacga cgcttcagca
    27301 acagcacgcc gccaatcaga cacagcacgc cgctggcacc accagcaaac atggccattt
    27361 tctgtttcac ttcaatcggc agccaggctt catacatcca gtgcggcgtc aacataccga
    27421 agaagtgacc gacaaaaatc cccagaatac cgatatggaa caggttcgac gccaggttca
    27481 tccctttgcg atccagcatc tggctggacg ccgcgcgcca ggtgtactgc ccgtagtcat
    27541 aacgcagcca gctaccaatc aggaagaccg ccccggctat gtacgggtag atatcaaaga
    27601 agaacatatt caggaattgc attagtgctg tcctccggtg gtgatattca gatattgcgg
    27661 cgcgacggca ccggcaaagc gacgctgatg agcagtgatt gctgaatcac cgcagccttt
    27721 gtcagcaaag aatttaacct gctcttcttc ccagacagca tccagcgcct gcggcgtatc
    27781 atcgcgcgct tcgtcggcaa ttttttccgc cactttgtcg ctgtcgatag cggtattggc
    27841 caatttcagc agcagatcaa acagcacggc ataacggctt tcacgctgtt gcagacgcgc
    27901 gctcagcaat gccagaatcg gcgcgatatc tttcaaacct tccacggctt cgctttgcgg
    27961 cagctgagcc aggtactcca gatacagcgg cagatggtcc ggcaattcgc ggctgtttaa
    28021 ctgcaagccg tgctgctcgt actgcgccaa caggtcaacc atcgcctggc cgcggtcgcg
    28081 ggattcgccg tgcacatgtt caaacagcaa cagcgacgtg gcgcgaccac ggtcgaacag
    28141 ttcgctgtac tgcgcctggg catccagcgg atccagcgcc gttaaatcgc gcaggaaaat
    28201 gcccagcgca tgggcatctt ctttcgacaa attttccgac gcggcaatcg cctcaaacat
    28261 ctcctgttga tgctgccata aggcagcatc cggatattca aggagacgcg atacaatcac
    28321 gagttcgatc atggatgcgg ctccgttttg ctggtcacat cgatggcatc gatacgacgg
    28381 ctgttgaaca gattgaattt ggtatctgag ccgtggcagc catcaccaaa ggtaaagccg
    28441 cagccatttt tctccgggaa ggcttcccgt gccagttcac gatgactact cggcaccaca
    28501 aagcgatctt cgtagttagc aatcgccaga taacggtaca tctcctgtgc ctgggcttca
    28561 gtcaggccga cctcttccag cgcacgggta tcaactttac cgtcaacggt ttcagcacgt
    28621 ttgtaatgac gcatcgccag catacgtttc agtgcgcgca gtaccggttt ggtatcaccg
    28681 gcggtcagca gattcgccag atactgtacc ggaatacgca agctttcgac gtctggcaga
    28741 attccgttgc tgcccagctc gcccgcgtct gctgcagact gaatcggaga cagaggcggc
    28801 acgtaccaga ccatcggcag tgtgcgatat tctggatgca gcggcagcgc cagtttccat
    28861 tccatcgcca ttttataaac cggcgactgc tgtgcagctt caataacgct cagcggaata
    28921 ccgtctttaa tcgcctgctc gatgactttc ggatcgttcg gatcgaggaa cacgtccagc
    28981 tgacgctggt agagatcttt ctcgttctcg gtgctggctg cacgttcaat cgcgtcggca
    29041 tcgtacagca gcacgccaag ataacggata cgaccgacac aggtttctga gcacacggtc
    29101 ggctgacccg cttcaatacg cggatagcag aagatgcact tctcagactt accgctcttc
    29161 cagttgaagt agattttttt gtacgggcat ccggtgatgc acatacgcca gccacggcat
    29221 ttatcctggt cgatcaggac gatgccatct tcttcacgct tgtaaatcgc accgctcggg
    29281 caggtcgcca cacatgccgg gttcaggcag tgttcgcaca ggcgcggcaa atacatcata
    29341 aaggtgtttt cgaactggct atacatcgcc ttctggatgt tgtcgaagtt cttgtctttc
    29401 gccagtttgt caaactcacc gcccaggtca tcttcccagt tcggcccttt ttcgattttc
    29461 gccatccgtt ccccggtaat cagcgaacgc ggacgggcaa tcggctgcgc tttgctgcct
    29521 tccggcgcgg tatgcaggtt ctgatagtca aaatcgaacg gctcgtaata atcgtcgatc
    29581 cccggcagat gcgggttagc gaagatttta cccagcagca tggcgcggtt acccatgcgc
    29641 ggctgcagtt tgccattgat tttacggatc cagccgcctt tgtatttttc ctggttttcc
    29701 cagtcagtcg ggaagccctg gcccggcttg gtttccacgt tgttgaacca cgcgtattcc
    29761 acgccttcac ggctggtcca gacgttttta caggtaactg aacaggtgtg gcagccgatg
    29821 cacttatcga gattcagcac catgccgact tgtgaacgaa ttttcatttt acgctctcct
    29881 gtacctggtc attgccttcg ccatctaacc agtcaatgtt cttcatctta cgcactacaa
    29941 caaactcatc gcggttagaa cctacggtgc catagtagtt aaagccgtat gccagatggg
    30001 catagccgcc gatcatatgc gtcggtttcg gcgtgatacg ggtgaccgag ttatggatac
    30061 caccacgctg ttgggtaatt tccgaaccag gcaggttaac gatacgttcc tgcgcgtggt
    30121 acatcatggt catccctgcc ggaacacgct ggctgacaac cgcacgggca gtcagagcac
    30181 cgttgctgtt gaatacttca atccagtcgt tatcggcgat acccagatct ttggcatcgg
    30241 cttcactcaa ccagaccacc ggaccaccgc gacctaaagt cagcatcagc aggttgtcgc
    30301 tgtaggtgga gtggataccc cacttctggt gcggcgtcag gaagttgagc gctttttccg
    30361 ggttgccatt ggatttctgg cccatcactt ctttcaccga acgggtgtcg atcggcggac
    30421 gataaaccag caggctttca ccgaaatcac gcatccactg gtgatcctga tacagttgct
    30481 gacgaccaga gagcgtacgc cacgggatca gctcgtgaac gttggtgtaa ccggcgttgt
    30541 aagaaacgtg ttcatcttcc agaccagacc aggtcgggct ggagatgatt ttgcgcggct
    30601 gtgcctgaat atcgcggaag cgaatcttct cgtcttcttt attcagcgcc agatgcgtat
    30661 ggtcacgacc ggtaaactcg ctcagggcag cccaggcttt cacggctacc tgaccgttgg
    30721 tttccggtgc cagcgtcagg atcatctctg ccgcatcaat tgcggtgttc agcatcggct
    30781 ggccttttgc cggaccttcc gctttggtgt agttgagctt acgcagcaga tccatctcgc
    30841 tctgggtgtt ccaggcaata cccttaccac cgttaccgat tttctccatc agcgggccga
    30901 tagaggtaaa gcgttcgtaa gttgccggat aatcgcgctc tacgaccata atgtgtggcg
    30961 cggttttacc tgggatcagg tcgcattcgc cttttttcca gtctttcaca tccagcggct
    31021 gcgccagttc agcggcagag tcgtgctgga taggcagcgt gacgatgtcg gtttctttac
    31081 ccagatggcc gacgcacact tcggagaatt tcttcgcgat ggctttgtag atttcccagt
    31141 cgcttttcgc ttcccaggcc ggatcgaccg ccgcagacag cgggtgaata aacggatgca
    31201 tatccgaagt attcatgtcg tctttttcgt accaggtcgc cgtcggcaaa atgatgtcgg
    31261 aatagagaca ggtgctcgac agacggaagt ccagcgtaac caccagatcc agcttgcctt
    31321 ccagaccatt gtcctgccag tccacttctt ccggcttcac gccgccctgc tgccccagat
    31381 ctttaccctg gataccgtgc tccgtcccca gcaggtactt gagcataaac tcatgacctt
    31441 taccggaaga accgagcagg ttagaacgcc agatgaacag gttgcgcggg tggtttttac
    31501 cgttttctgg ttgttctgcc gcaaaacgga tggaaccctc tttcagggat ttcacggtgt
    31561 aatctaccgg attcatcccg gctttttccg cttcgccagc gatagtcagc gggttagtgc
    31621 ctaactgcgg tgcagacggc agccagccca tgcgttctgc acggacgtta aagtcgatta
    31681 ggtgtccggt ataacgggat ttatccgcca tcggcgacag cagttcttcc gcagtcacgg
    31741 tttcataacg ccactggctg gagtggttat agaaataaga agtgctgttc atgtgacgcg
    31801 ccggacgctg ccagtcaagg gcaaacgcca gcggctgcca gccggtttgc ggacgcagtt
    31861 tttcctgacc tacatagtgc gcccagccgc ccccgctctg accgacacag ccgcagaaaa
    31921 tcagcatgtt gatcagacca cgatagttca tatcgaggtg ataccagtgg ttcagccccg
    31981 caccgacgat aatcatcgaa cgaccgtgcg ttttatcagc gttatcggca aattcacggg
    32041 cgatgcgaat aatctggctg cgagaaacgc cggtaatctg ctcggcccag gccggggtat
    32101 aagctttcac atcgtcatag ctggttgcac agttaacgtc gttcaggcca cgttccagac
    32161 cgtagtttgc cagcgtcaga tcataaacgg tggtcaccag ggcggtgctg ccatcagcca
    32221 gttgcaggcg tttcaccggc agtttgtgca gcagcacgtt ttccagttcc actttgttga
    32281 agtgttcagt accgtcgcca ccaaagtacg ggaagcccac ctcagcgatc tcatcctggc
    32341 tacccagcag gctcagttgc agctcggttt cttcgccagt tttgccgtcg cgctgctcaa
    32401 gattccattt gcccttctcg ccccagcgga agccaataga accgttcggc gcaaccattt
    32461 caccattggt attaaaggcg acagttttcc attccggatt gttttcctga cccagcgcat
    32521 ccaccagatc agcagcgcgc agcatacgac ccgcggcgta gtaaccgtcg cgctcttcca
    32581 gcatcaccag catcggcatg tcggtgtagc gacgcacata gtcggtgaaa tactggcttg
    32641 ggttgtcgag gtggaattca cgcagcatta cgtggcccat tgccagcgcc attgccgcat
    32701 cggtgccctg tttcggtgcc agccacagat cgcacagttt ggcgatttca gcgtagtccg
    32761 gcgtgacggc aacagttttg gtccctttgt aacgcacttc agtaaagaag tgagcatccg
    32821 gggtacgcgt ctgcggcacg tttgaccccc aggcgatgat gtagctggag ttgtaccagt
    32881 cagcagattc cggtacgtct gtttgctcgc cccaggtttg cggagacgca ggaggcaggt
    32941 cgcagtacca gtcgtagaag cttaagcaag taccgccaat cagcgagaga tagcgtgcac
    33001 ccgatgcgta agaaaccatc gacattgccg gaattggcga gaagccagcg acacggtccg
    33061 ggccgtagtt tttgatggtg taaacgttag atgcggcgat cagttcgttc acttcctgcc
    33121 aggaagaacg aacaaaacca ccgcgtccac gcgcctgctt aaagcttttc gctttatcgg
    33181 cgtcttcaat gatagaagcc catgcctcaa ccggatcgct atgcagcgcc ttcgcttcac
    33241 gccacatttt catcaagcgt ttgcgcatca tcgggtattt caggcggttg gcgctgtaaa
    33301 gataccagga gtagctggca ccgcgcgggc agccgcgagg ttcatggttt ggcaggtccg
    33361 ggcgagtacg tggatagtca gtctgctggg tttcccaggt gaccagaccg tttttgacgt
    33421 agattttcca gctgcaggag ccggtgcagt ttaccccgtg ggtagagcgg acgattttgt
    33481 catgctgcca acgctggcga tatccatcct cccagtcacg gttggtattg agaagctggc
    33541 catgcccatc ggcaaaggtt tcacccttct gtttgaagta gcgaaaccgg tccaggaatt
    33601 tactcatcgg ttttctcctg tgggagcctg tcggccctct gataaatcga cattgctaat
    33661 gtttaagggc aaggtaacgc tctgaaacag cgtgggaatt gataacgatc aagtgtgatg
    33721 gggatgaaaa ataaagtaaa ttcctaagat acccctttag gataat
//
LOCUS       DAZFWP020000039        32584 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFWP020000039 DAZFWP020000000
VERSION     DAZFWP020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.039"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..1370
                     /gene="hpaG"
                     /locus_tag="ACHK0Z_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     CDS             81..1370
                     /gene="hpaG"
                     /locus_tag="ACHK0Z_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HGU4501744.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARSVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1367..2833
                     /gene="hpaE"
                     /locus_tag="ACHK0Z_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     CDS             1367..2833
                     /gene="hpaE"
                     /locus_tag="ACHK0Z_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4501745.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2835..3686
                     /gene="hpaD"
                     /locus_tag="ACHK0Z_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     CDS             2835..3686
                     /gene="hpaD"
                     /locus_tag="ACHK0Z_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HGU4501746.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3696..4076
                     /locus_tag="ACHK0Z_RS08560"
                     /old_locus_tag="pgaptmp_001712"
     CDS             3696..4076
                     /locus_tag="ACHK0Z_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HGU4501747.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4187..4990
                     /gene="hpaH"
                     /locus_tag="ACHK0Z_RS08565"
                     /old_locus_tag="pgaptmp_001713"
     CDS             4187..4990
                     /gene="hpaH"
                     /locus_tag="ACHK0Z_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HGU4501748.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5001..5789
                     /gene="hpaI"
                     /locus_tag="ACHK0Z_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     CDS             5001..5789
                     /gene="hpaI"
                     /locus_tag="ACHK0Z_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HGU4501749.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            5964..7340
                     /gene="hpaX"
                     /locus_tag="ACHK0Z_RS08575"
                     /old_locus_tag="pgaptmp_001715"
     CDS             5964..7340
                     /gene="hpaX"
                     /locus_tag="ACHK0Z_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HGU4501750.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7350..8240
                     /gene="hpaA"
                     /locus_tag="ACHK0Z_RS08580"
                     /old_locus_tag="pgaptmp_001716"
     CDS             7350..8240
                     /gene="hpaA"
                     /locus_tag="ACHK0Z_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HGU4501751.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8491..10053
                     /gene="hpaB"
                     /locus_tag="ACHK0Z_RS08585"
                     /old_locus_tag="pgaptmp_001717"
     CDS             8491..10053
                     /gene="hpaB"
                     /locus_tag="ACHK0Z_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HGU4501752.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10071..10583
                     /locus_tag="ACHK0Z_RS08590"
                     /old_locus_tag="pgaptmp_001718"
     CDS             10071..10583
                     /locus_tag="ACHK0Z_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HGU4501753.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10976..13126
                     /gene="btsT"
                     /locus_tag="ACHK0Z_RS08595"
                     /old_locus_tag="pgaptmp_001719"
     CDS             10976..13126
                     /gene="btsT"
                     /locus_tag="ACHK0Z_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU4501754.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13176..13379
                     /gene="yjiX"
                     /locus_tag="ACHK0Z_RS08600"
                     /old_locus_tag="pgaptmp_001720"
     CDS             13176..13379
                     /gene="yjiX"
                     /locus_tag="ACHK0Z_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4501755.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13390..14346
                     /gene="yjiA"
                     /locus_tag="ACHK0Z_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             13390..14346
                     /gene="yjiA"
                     /locus_tag="ACHK0Z_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4501756.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14424..16964)
                     /locus_tag="ACHK0Z_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             complement(14424..16964)
                     /locus_tag="ACHK0Z_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HGU4501757.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17316..17480)
                     /gene="yjiS"
                     /locus_tag="ACHK0Z_RS08615"
                     /old_locus_tag="pgaptmp_001723"
     CDS             complement(17316..17480)
                     /gene="yjiS"
                     /locus_tag="ACHK0Z_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU4501758.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17657..19069
                     /gene="yjiR"
                     /locus_tag="ACHK0Z_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             17657..19069
                     /gene="yjiR"
                     /locus_tag="ACHK0Z_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4501759.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19311..20255)
                     /locus_tag="ACHK0Z_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             complement(19311..20255)
                     /locus_tag="ACHK0Z_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4501760.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20722..21954
                     /gene="mdtM"
                     /locus_tag="ACHK0Z_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             20722..21954
                     /gene="mdtM"
                     /locus_tag="ACHK0Z_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU4501761.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVGDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTESRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21995..23275
                     /gene="yjiN"
                     /locus_tag="ACHK0Z_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             21995..23275
                     /gene="yjiN"
                     /locus_tag="ACHK0Z_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU4501762.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23391..24542
                     /gene="yjiM"
                     /locus_tag="ACHK0Z_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             23391..24542
                     /gene="yjiM"
                     /locus_tag="ACHK0Z_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU4501763.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24552..25319
                     /gene="yjiL"
                     /locus_tag="ACHK0Z_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             24552..25319
                     /gene="yjiL"
                     /locus_tag="ACHK0Z_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU4501764.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25316..25573
                     /gene="ytiA"
                     /locus_tag="ACHK0Z_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             25316..25573
                     /gene="ytiA"
                     /locus_tag="ACHK0Z_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU4501765.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            25638..26498
                     /gene="yjiK"
                     /locus_tag="ACHK0Z_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             25638..26498
                     /gene="yjiK"
                     /locus_tag="ACHK0Z_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4501766.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26566..27744
                     /gene="yjiJ"
                     /locus_tag="ACHK0Z_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             26566..27744
                     /gene="yjiJ"
                     /locus_tag="ACHK0Z_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4501767.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27757..28311)
                     /gene="kptA"
                     /locus_tag="ACHK0Z_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             complement(27757..28311)
                     /gene="kptA"
                     /locus_tag="ACHK0Z_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGU4501768.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28561..29244
                     /gene="yjiH"
                     /locus_tag="ACHK0Z_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             28561..29244
                     /gene="yjiH"
                     /locus_tag="ACHK0Z_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU4501769.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEMTERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29241..29702
                     /gene="yjiG"
                     /locus_tag="ACHK0Z_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             29241..29702
                     /gene="yjiG"
                     /locus_tag="ACHK0Z_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU4501770.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLIGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29715..30887
                     /gene="iadA"
                     /locus_tag="ACHK0Z_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             29715..30887
                     /gene="iadA"
                     /locus_tag="ACHK0Z_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4501771.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30952..31863
                     /gene="hypT"
                     /locus_tag="ACHK0Z_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /gene_synonym="qseD"
     CDS             30952..31863
                     /gene="hypT"
                     /locus_tag="ACHK0Z_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU4501772.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31856..32248)
                     /gene="iraD"
                     /locus_tag="ACHK0Z_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             complement(31856..32248)
                     /gene="iraD"
                     /locus_tag="ACHK0Z_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU4501773.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(32245..32328)
                     /gene="idlP"
                     /locus_tag="ACHK0Z_RS08695"
                     /old_locus_tag="pgaptmp_001739"
     CDS             complement(32245..32328)
                     /gene="idlP"
                     /locus_tag="ACHK0Z_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU4501774.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(32444..>32584)
                     /locus_tag="ACHK0Z_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             complement(32444..>32584)
                     /locus_tag="ACHK0Z_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501775.1"
                     /translation="ADYAEINNFPPELSSGGDKYFHLHNYSEYSEYTSGFFLSLMIFI
                     KS"
BASE COUNT         7482 a         8612 c         8552 g         7938 t
ORIGIN      
        1 ttttgccaaa ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg
       61 atcgaatccg aggagtggta atgaaaggca ctatcttcgc cgtagcattg aaccatcgca
      121 gccagcttga tgcatggcag gaagcgttcc agcaatcccc ctacaaagcc ccgcctaaaa
      181 ctgcggtctg gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct
      241 ttccacaggg tgaaaaggta ctgagcggtg cgacagttgc gctgattgtg ggaaaaacgg
      301 cgacgaaagt acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg
      361 acgtcagcct gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg
      421 gattctgccc cattggcgaa accgtggctc tcagcaatgt cgataatctg accatctata
      481 ccgagatcaa cgggcgtcct gccgatcact ggaacaccgc cgatttacaa cgtaacgccg
      541 cgcagttgct gagcgccctg agcgaatttg ccacactgaa tccaggcgat gccattctgc
      601 tcggcacgcc acaggcgcgc gtggaaatac agccaggcga tcgcgttcgt gttctcgcag
      661 aaggtttccc gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga
      721 gcttcccaac gcagccacac ccgcacggca cgctgtttgc cctcggcctg aactacgccg
      781 accacgccag cgaactggaa tttaagccac cggaagaacc gctggtgttc ctgaaagcgc
      841 caaataccct cactggcgat aaccagacct ccgtgcgtcc aaacaatatt gaatacatgc
      901 actatgaagc ggagctggtg gtagttattg gcaaacaggc gcgtagcgtc agcgaagccg
      961 atgccatgga ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc
     1021 tggaaaacta ctaccgccct aacctgcggg taaaaagccg cgacggactg acgccgatgc
     1081 tttcaaccat cgtgccgaaa gaggcgatcc cggacccgca taatctgacc cttcgcacct
     1141 tcgtcaacgg cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct
     1201 tcctgatcgc ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg
     1261 gcacaccaaa aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag
     1321 gcgtgggccg cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa
     1381 tcattggatc aacggcaaaa atgttgcagg taacgactac ttccagacca ccaatccggc
     1441 aacgggtgaa gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt
     1501 agcggcagcg aaagaggcgt tcccgaaatg ggccaatctg ccaatgaaag agcgtgcgcg
     1561 cctgatgcgc cgtctgggcg atctgatcga ccagaacgtg ccagagatcg ccgcgatgga
     1621 aaccgcggac accggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc
     1681 ccacaacttt gaatttttcg cggaagtctg ccagcagatg aacggcaaga cttatccggt
     1741 cgacgacaag atgctcaact acacgctggt gcagccggta ggcgtttgtg cactggtgtc
     1801 accgtggaac gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg
     1861 caataccgcg gtactgaaaa tgtcggaact ctccccgctg accgccgacc gcctgggtga
     1921 gctggcgctg gaagctggta ttccggcggg cgtgctgaac gtggtacagg gctacggcgc
     1981 aaccgcaggc gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg
     2041 tacggcgacc gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggaact
     2101 gggcggtaaa tcgccggtgc tgatttttga agatgctgat attgaacgcg cgctggacgc
     2161 cgccctgttc accatcttct cgatcaacgg cgaacgctgc accgccggtt cgcgcatctt
     2221 tattcagcaa agcatctacc cggaattcgt taaacgcttt gccgaacgcg ccaaccgtct
     2281 gcgcgtgggc gatccgaacg atccgaatac ccaggttggc gcgcttatca gccagcagca
     2341 ctgggaaaaa gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgctggc
     2401 gggcggcccg gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg
     2461 cccaaccgtg ctggcagacg ttgataaccg tatgcgagtc gcccaggaag agattttcgg
     2521 gccggtcgcc tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgtc tggcaaacga
     2581 cgtggagtac ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct
     2641 ggcgcgtggc attgaagctg gcatggtgtt cgtcaacacc cagaacgtgc gtgacctgcg
     2701 ccagccattt ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt
     2761 cgaagtgttc gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa
     2821 atggggagtc tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga
     2881 tgtatctctc tgaactgcca gggaaaaacc acggttgccg ccagggcgcg atcgacgggc
     2941 ataaagagat cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata
     3001 cccactggct ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct
     3061 acaccagcaa cgagctgccg cattttatcc gcgatatgac ctacaactac gagggcaacc
     3121 cggagttggg gcagcttatt gccgatgaag ccttaaagct cggcgtgcgg gcaaaagcgc
     3181 acaacattcc cagcctgaaa ctggaatacg gcacgctggt gccaatgcgc tacatgaatg
     3241 aagacaagca cttcaaagtg gtttccattt cagctttctg cactgttcac gattttgccg
     3301 acagccgcaa gctgggcgag gcgattctga aagcgatcga acagtacgac ggcaccgtgg
     3361 cggtccttgc cagcggttcg ttatcgcacc gctttattga cgatcagcga gcggaagaag
     3421 ggatgaacag ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt
     3481 ggcgcgaagg ccagttcaaa gagttctgca atatgctgcc ggagtacgcc gactactgct
     3541 acggcgaagg caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat
     3601 acgacggcaa ggtggagttt atcaccgagc tattcccaag ctctggcacc ggtcaggtta
     3661 acgctgtttt cccgcttccc gcgtaaggac gttttatgcc gcactttatc gttgaatgca
     3721 gtgacaacat ccgcgaagaa gccgacctgc cggggttgtt cgccaaagtg aatccgacgc
     3781 tggcagccac gggtattttt ccgctggcgg gtattcgcag ccgcgtgcat tgggtcgata
     3841 cctggcagat ggccgacggg cagcgtgatt atgccttcgt gcatatgacg ttgaaaatcg
     3901 gcgcaggtcg cagcctggaa agccgtcagc aggcgggtga aatgctgttt gaactgatta
     3961 aaacgcactt cgccgccctg atggagagcc gcctgctggc gttgtcgttt gagattgaag
     4021 agctgcatcc gacgctgaat tttaaacaaa acaacgtgca cgcattgttt aagtagcgcg
     4081 cagattgccc ggtggcgctg cgcttaccgg gcctacaaaa ccccaaaccg tacaccgtag
     4141 gccggataag gcgcagccgc atccggcaat gccacaggat atcgctatgt tcgataaaca
     4201 tacccacacc ctgatcgccc agcgtctgga tcaggcagaa aaacagcgcg aacagatccg
     4261 cgcgatctca ctggattacc cggagatcac catcgaagac gcttacgcgg tgcagcgcga
     4321 atgggttcgc ctgaaaatcg ccgaaggtcg cacgctgaaa ggccacaaaa tcggcctgac
     4381 ctcgaaagcg atgcaggcaa gctcgcagat cagcgaaccg gattacggcg cgctgctgga
     4441 tgacatgttc ttccacgatg gcagcgatat cccgaccgat cgctttatcg tgccgcgcat
     4501 tgaagtggag ctggctttcg tgctggcaaa accgctgcgt gggccaaact gcacgctgtt
     4561 cgacgtctac aacgccacgg actacgtgat cccggcgctg gagctgatcg acgctcgctg
     4621 ccacaacatc gatccggaaa cccagcgccc gcgcaaagtg ttcgacacca tttctgataa
     4681 cgccgccaac gccggggtga tcctcggtgg tcgtcccatt aagcccgatg agttggatct
     4741 gcgttggatc tccgccctga tgtatcgcaa tggcgtgatt gaagaaaccg gcgtcgccgc
     4801 tggtgtgctg aatcatccgg caaacggcgt ggcctggctg gcgaacaaac tcgcaccgta
     4861 tgacgtacaa ctggaagccg ggcaaatcat tctcggcggt tcgttcaccc gcccggttcc
     4921 ggcgcgtaag ggcgacacct tccacgtcga ttacggcaac atgggctcca ttagctgccg
     4981 ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa gcaggccgcc
     5041 cgcagatcgg attatggctg gggctgagta gcagctacag cgcagagtta ctggccggag
     5101 caggattcga ctggttattg atcgacggtg agcacgcgcc gaataacgtg caaaccgtgc
     5161 tcacccagct acaggcgatt gcgccctacc ccagccagcc ggtggtacgt ccgtcgtgga
     5221 acgatccggt gcaaatcaaa caactgctgg acgtcggcac acaaaccttg ctggtgccga
     5281 tggtacaaaa cgctgacgaa gcccgtgaag cggtacgcgc cacccgttat ccccccgccg
     5341 gtattcgcgg tgtgggcagt gcgctggccc gagcctcgcg ctggaatcgt attcctgatt
     5401 acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa
     5461 tgaagaactt accgcagatt ctggacgtgg aaggggtcga cggcgtgttt atcggcccgg
     5521 cggatctgag cgccgatatg ggttatgccg gtaatccaca gcacccggaa gtacaggccg
     5581 cgattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg atcctgatcg
     5641 ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc gctgtcggcg
     5701 ttgacaccac cctgctcgcc cgcgccgctg aagcgctggc agcacggttt ggcgcacagg
     5761 ccacgaccgt taagcccggc gtgtattaat gccggatgcg ctgcacttat ccggcctaca
     5821 ctcgcaccaa accgtaggcc ggataaggcg tttacgccgc atccggcaaa aagccgtacc
     5881 aaatcgcgga ccggataagg cgtttgtgcg catccgccat aaaccttgtc gtaccctaca
     5941 aaaatcccat tagaggaaga aaaatgagcg acacctcacc tgccataccg gagagtatcg
     6001 atccggcgaa tcagcataaa gcgctgactg ccggacaaca ggcggttatt aagaagctgt
     6061 ttcgccgcct gatcgtcttt ctgttcgtgc tgtttatctt ctcgttcctt gatcgcatca
     6121 acatcggctt tgccggactc acgatgggac gcgacctcgg tctgagcgcc accatgtttg
     6181 gcctcgctac caccctgttc tatgccgctt atgtcatctt cggcattccc agcaacatta
     6241 tgctgagtat tgtcggtgcg cggcgctgga tcgccaccat catggtgctt tggggcatcg
     6301 cctctactgc caccatgttt gccactggcc ccaccagctt gtacgtactg cgtatactgg
     6361 ttggcattac cgaagccggc tttctgcccg gcattctgct gtatttaacc ttctggtttc
     6421 cggcctattt ccgcgcccgt gccaacgcct tgtttatggt agcaatgccg gtaacgacag
     6481 cgttgggctc gatcgtttcc ggctacattt tgtcgctgga tggcgtcatg gcattaaaag
     6541 gctggcagtg gctgtttttg ctggaaggct tcccgtcggt attactcggc gtcatggtgt
     6601 ggttctggct tgatgactca ccggacaaag ctaagtggct gacgaaagaa gacaaaaaat
     6661 gcctgcaaga gatgatggat aacgatcgtc tgacgctggt tcagccagag ggagccatca
     6721 gccaccacgc catgcaacaa cgcagtatgt ggcgggagat cttcactccg gtggtgatga
     6781 tgtataccct ggcgtatttc tgcctgacca acacacttag tgcgatcagc atctggacac
     6841 cgcagatcct gcaaagtttt aatcagggca gcagtaatat caccatcggc ctgctggccg
     6901 ccgtaccgca gatttgtacc attctcggga tggtctactg gagccgtcac tcagatcgcc
     6961 gccaggaacg aaggcatcac accgcccttc cttatttgtt cgctgccgca ggttggttac
     7021 tggcttcggc aactgatcac aacatgatcc agatgctggg gatcattatg gcttcgaccg
     7081 gatcattcag cgcaatggcg attttctgga caacaccgga tcagtccatc agcctgcggg
     7141 cacgagcgat cggtattgcg gtgatcaacg ccactggcaa cattggttca gcgttaagtc
     7201 cgtttatgat cggctggttg aaagatttga ccggcagctt taacagtgga ttgtggtttg
     7261 ttgccgcgct gctggtgatt ggtgcgggga ttatctgggc gattccaatg cagtcctccc
     7321 gtccgcgagc gaccccgtaa ggaacgacga tgtgtgaccg tcagattgcc aatattgata
     7381 tcagcaaaga gtacgatgaa agcctgggca cggacgatgt gcattatcag tccttcgccc
     7441 gcatggcggc cttttttggc cgccatatgc tgccacatcg ccacgaacag tactttcaga
     7501 tgcatttcct caatagcgga cagattgagc tacaacttga cgatcatcgc tactcggtgg
     7561 aagcgcccct gtttgtcctg acgccgccgt cagtacctca tgcgtttatt acggagtctg
     7621 acgccgacgg tcatgtgttg acggtacggg aagatctgat ctggcccctg ctggaagttc
     7681 tttatccggg cactagggaa accttcggcc tgccggggat ttgcctgtca ctggcagata
     7741 aacccgacga actggcggcg ctggaacact attggcaact gatagagcgg gaatcggtgg
     7801 aacaactgcc tggacgggaa cacaccctga cgttactggc acaggcagtg ttcaccctac
     7861 tgctgcgtaa cgcaaaactc gacgaccatg ccgccagcgg gatgcgcgga gaattaaaac
     7921 tgttccagcg ttttaatatg ctgattgaaa gccattttca tcagcactgg acagtaccgg
     7981 attacgctaa cgaactgcat atcaccgaat cacgactcac ggacatctgc cgtcgctttg
     8041 ccaaccgtcc gccaaaacgg ttgattttcg acaggcagct gcgagaagcc aagcggctgc
     8101 tgctgttttc tgataacgcc gtgaacaata ttgcctggca actcggtttt aaggatccgg
     8161 cttattttgc gcgctttttt aatcgcttag tcggttgctc gcccagtgct tatcgcgcca
     8221 aaaaagtacc tgtgacgtga aaatccctta gccttaacgg gaaaccaggc accacctgct
     8281 attccccttt ctccgcctgg agcaagggga tttagtaacg tcaaaaatcg ctaaaagcga
     8341 cctcgatcac aaaaccgtac ccgacctgaa aagtaccagc cattgtccca aaagtctctt
     8401 cccgtaatcg cccctgcaag cttcaattga ataacaaaaa caacacacaa aattaacaat
     8461 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     8521 caacgtccgt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     8581 atctatggcg agcgagtgaa agacgtcact actcatccgg catttcgtaa tgcggcagcg
     8641 tctgttgccc aactgtacga cgcgctacac aaaccggaga tgcaggactc tctgtgctgg
     8701 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     8761 gacgacctgc gccagcaacg cgatgccatc gctgagtggt cacgcctgag ctatggctgg
     8821 atgggccgta ccccagacta caaagctgct ttcggttgcg cactgggcgc gaatccgggc
     8881 ttttacggtc agttcgagca gaacgcccgt aactggtaca cccgtattca ggaaactggc
     8941 ctctacttta accacgcgat tgttaaccca ccgatcgatc gtcatttgcc gaccgataaa
     9001 gtgaaagacg tttacatcaa gctggaaaaa gagactgacg ccgggattat cgttagcggt
     9061 gcgaaagtgg ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg
     9121 gcacaagtaa tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc
     9181 aatggcgtca aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg
     9241 ccgtacgact acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat
     9301 aacgtgctga tcccatggga aaacgtactg atctaccgcg atttcgatcg ctgccgtcgc
     9361 tggacgatgg aaggcggttt tgcccgtatg tatccgctgc aagcctgtgt gcgcctggca
     9421 gtgaaactag acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg
     9481 gagttccgtg gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg
     9541 gcattgagtg actcgatgtg ttctgaagcg acgccgtggg tcaacggggc ttatttaccg
     9601 gatcatgccg cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa
     9661 aacattatcg aacgcaacgt taccagtggc ctgatctatc tcccttccag tgcccgtgac
     9721 ctgaacaatc cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaatggtatg
     9781 gatcacgtcc agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgagttt
     9841 ggtggtcgcc acgaactgta tgaaatcaac tactccggta gccaggatga gattcgcctg
     9901 cagtgtctgc gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat
     9961 cgctgcctgt cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac
    10021 gatatcaaca tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag
    10081 atgaacagcg cctgcgcttt cgtgacgcga tggccagcct gtcggcagcg gtaaatatta
    10141 tcaccaccga gggcgacgcc ggacaatgcg ggattacggc aacggccgtc tgctcggtca
    10201 cggatacacc accatcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt
    10261 ttcagggcaa cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcac
    10321 gccacttcgc gggcatgacg ggcatggcga tggaagagcg ttttagcctc tcatgctggc
    10381 aaaaaggtcc gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggtgaga
    10441 tccgcgatgt gcaggcaatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca
    10501 tcctcagtgc agaaggtcac ggacttatct actttaaacg ccgtttccat ccggtgatgc
    10561 tggaaatgga agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt
    10621 ttattgtgat ctcaatcaca tatccacccg atgaataatt cttgcccaac cgctcaccgc
    10681 cattcctgac cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc
    10741 gcgtaactct ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc
    10801 aaacctagaa cggcttcggc ctatgattaa caaattactc tatgaatttt ggtttgcagg
    10861 aaggcagcaa gtgagtgaat ccccgggagc ttacaacagt aagtgacagg ggtgaacgaa
    10921 cgcaactgcc gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga
    10981 tacgaaaaaa ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg
    11041 cctcgcggta gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc
    11101 ctctgtgtcg gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt
    11161 gatgaaactc gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta
    11221 cgttccgacc aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg
    11281 tccgctggtc ggtccggttc tcgccgcaca gatgggctac ctgcctggca cgctgtggct
    11341 gctggcgggg gtagtactgg ccggtgcggt tcaggacttt atggtgctgt ttatctcctc
    11401 tcgccgtaac ggcgcatctc tcggtgagat gatcaaagaa gagatgggac cagtaccggg
    11461 gactatcgcg ctgtttggct gtttcttaat catgatcatc atcctcgccg tcctggcgct
    11521 gattgtggtt aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt
    11581 accgattgcg ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga
    11641 agtctctgtc attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc
    11701 tcacgatccg tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct
    11761 gattggctat gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga
    11821 ctatctggca accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt
    11881 gctgaacccg gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct
    11941 gtggaaaggc gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg
    12001 cttccacgcg ctgatctctt ccggtacgac gccaaaactg ctggctaacg aaaccgacgc
    12061 gcgtttcatt ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt
    12121 tgcagcgtcc atcatcgaac cgggtcttta cttcgcgatg aataccccgc ctgctggcct
    12181 tggcatcacc atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc
    12241 gcagctgaaa gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat
    12301 ttcgccagag cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg
    12361 tgcaggtggt gcgccaacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc
    12421 gatggctgac atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct
    12481 gaccgcgctg gatgcgggta ctcgttctgg ccgctttatg ctgcaagacc tgctgggtaa
    12541 cttcatcccg ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg
    12601 ctgtgtgggt ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt
    12661 taagagcctg tggccgctgt tcggtatttc caaccagatg ctggcagccg tagcgctggt
    12721 actgggcacc gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt
    12781 tccggctgta tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac
    12841 caacccgcag atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa
    12901 cggtactgac ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta
    12961 caccaacgcg ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg
    13021 tttcaaaacc tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta
    13081 cgttccaatc ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt atttagcccc
    13141 gcttcggcgg ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa
    13201 aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactat gacaactacg
    13261 tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc
    13321 gcgaacgcca gaacgcacgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat
    13381 ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa
    13441 accaccctgc tgcgccatat tcttaatgaa caacacggct acaagattgc cgtgattgaa
    13501 aacgaattcg gcgaagtctc cgttgatgat caattgattg gtgatcgcgc aacgcagatc
    13561 aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcgcta
    13621 ctcgacctgc tggataatct cgacaagggc aatattcagt tcgatcgtct ggtcattgaa
    13681 tgcaccggca tggccgatcc tggcccgatt attcaaacct ttttctccca tgagatttta
    13741 tgccagcgct acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag
    13801 cagatgaacc agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg
    13861 actaaaaccg acgtcgcagg cgaagcagaa aaactgcgtg aacgcctggc gcgcatcaac
    13921 gcccgtgcac cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac
    13981 accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt ccactttatc
    14041 gcggataaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc
    14101 agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt
    14161 tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag
    14221 cgcctctaca gcgccgactg ggacaggcca tggggcgatg aaacaccgca tagcacgatg
    14281 gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc gggactcaaa
    14341 aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat attcctccag
    14401 aatatgaggg ctttcgggct taattatctc tgaacgtgca acacttcctg cgactcctta
    14461 tgcgcctcca catcctgctt cgttaaccag agcgacttac ggccaaaatt ttcgtacatt
    14521 ttcagctgat cttcatagtg cttatcaact gttccatcgg gagcaataaa cccactctga
    14581 ccgggtgcga ccacatccca ggcaagcaca ggacgatcgc ttgtcgttgg tgagaaaaca
    14641 atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt ttcttccgct
    14701 gcggcctgcg gtacaccaaa gaaattattt gcccggaacg ttaaggccat tgcaggtgtt
    14761 ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt atcttccagc
    14821 gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc ctgtgggatt
    14881 ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact tatattcagc
    14941 gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca cttatcaaat
    15001 ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca aacgttcagg
    15061 atggcagagc ctggctgctg ccaggtttta ccatcatcat taagcaaatt gatgccatcc
    15121 caacgtgtta atgtttctac caactgacga cgcggatcgc tctgtgtcaa accagatgtc
    15181 gctgcttgca gagtaggtaa aaaaagcctc aggttaagat cctgacgact ggtttggcga
    15241 ataacatccc atgcctgatc agcagttaag cgtggctttt gctcaagcag tcggtcgatc
    15301 tccgtaacgc gatctgcacc accccacaaa aaggcaaaca gatctgaagc gggataatct
    15361 ttttggggag aattgttcca gttagcaata tatcccgact gggggttata caccttaggg
    15421 ttcatttcaa aaggcaatag ccctttccag tcccattttc ccgtaccagg aacgggtaat
    15481 cgcggatcat ggcctgattg acgatctgga taagcaccag tatgaacata accaatattg
    15541 ccgtttacat cagcatagta ccagttgatg gtcagtgctt gtttcgctgc ctgctgtgtc
    15601 cactcctgcc aatttttggc cttcatctga tgagtccagg ccagcaaaga cgccacctct
    15661 ttaccatccc atgcgcggga tttagcgtaa gccgtttgtg tcgtctggtc agtttggaga
    15721 atgttgccat gcaccgtacg ccagacagta aaggtctctg cctgaccatt tttcaccgta
    15781 atggtttcct cacggcttaa cattttcacc cacttaccat tatgcaagta gtagcctggt
    15841 ttctctgccg acagccgttc agcaaaaata tcgacatcat cgccgaaacc tgccgttgat
    15901 ccccaggaaa tcacaccatt atgaccaaaa accagcccag gataggcaaa tggtgtattg
    15961 ccagtgacat cataaccagc accgtgcaga ccaataccat aagtatacgc aggcgcatac
    16021 cagccaaact gcggaccatt taccatgatt gctttcgcat cctgggcttt gcttttgccg
    16081 atcacccaca tattgctggt cgttggatac cccgccagac cattggcacc accctgtgca
    16141 aattgtgcag caatagtttc ccggttcttc cctgctgtta acgccagcag tgcgccatcc
    16201 gccccttttg ctggtcggtc aagcattggt gcaggtaaat cgtagcgtgg caacagagct
    16261 gctgtttgcg agttttgctg attaaattta agtgggtagt tactctcttg tacggcaata
    16321 gtggttggcg ctgatgggtt taccagccat ttcaactgat taaataccgc catgccttgt
    16381 gatacaccat atttatcttt taaagccgtt agcagtgcca gattatcaat ttcgctagtg
    16441 ctatcagaga agcggtttgc catggtgccc acaaatatca tcgcgacatc aaacggttcc
    16501 cagcgcttag gagtaaagcc aaatgtatta aactgttttg gtaagagcgt ctctggattg
    16561 gtatttacct tatcaatcca ggcattcatt ccatcagcgt agccttgcag aatggacata
    16621 tcctctgggg aaagggcagc aatttgcgcc cggatagcat ccggccagta gttacgacgg
    16681 atatctttat caaatttcac aaaatctttg ccaagcactt ccgcgacagt cccttgagta
    16741 ctgcgacgtg ccatttccat ctgaaaaagg cgatcttgtg ctactacata gccatagcca
    16801 taaaataggt gccatgtatc attggcataa atatgcggca tgccgtattc atcgcgaaca
    16861 atctttatct cacttgacga ctgctcagcc agtgcaggta agctccaata atacatcagg
    16921 gaagcagtaa cacagttcac gatcatacga tttctatttt tcattgtatc ctctggcagg
    16981 tgtataatta gcgaattgat actagcaacg aagcttcggt tatctatttg cagagaattg
    17041 tttcattatg aactgtgagc gcgataacta atttttgata caaaaatttc attaatgtga
    17101 tctaaaacga atcgctttat tcaccgctga tgattttgtt ctattccttt ttgtgacaga
    17161 tcccccggac gcatagatga tgacccccag agagacagct caggtcatta tctgtatgta
    17221 aatcagaaaa atatgttgta aacgactttt atggttggat tttgtagcaa ttttgaatgc
    17281 agacaggcag catttgccaa cacgccgccc atagttcact ccacatcctc cctgcgtaac
    17341 ccaatatccc tcaaccgttc atcactcatc tgctgtaaca ctcgtcgggt ctgcatttgc
    17401 cgccgccaac gcctcaccgc atgccagagt tgcacaagtc tgataaacgg cgttttggct
    17461 ttgttttcgt gaaattccat ctgctgcctc ctcatctttc agaggcttca tcatccgcct
    17521 tctggcagtt cacaatacag atgcaaatat tagttttatt taacatacag acaggttaaa
    17581 acagcatctg catccggttt tttagcctca tctgtaccgg tttttctttc tgtatgggcc
    17641 aaataaggaa tacagcatga cgcgttatca acatctggcg actctacttg ccgagcggat
    17701 tgagcaaggg ctgtatcgtc acggggagaa attgccgtcg gtgcgcagct taagtcagga
    17761 gcacggcgtc agcatcagca ccgtgcaaca ggcgtatcag acgctggaaa cgatgaggct
    17821 catcactccg cagccgcgtt cgggttattt tgtcgcacaa cgtaaagccc agccgcctgt
    17881 accgccgatg acgcgtccgg tgcagcgccc ggtggaaatt acccagtggg atcaggtgct
    17941 ggatatgctg gaggcgcata gcgacaattc cattgttccg ttaagcaaaa gcacgccgga
    18001 tgtcgaaacg cccagcctga aaccgctgtg gcgtgagcta agccgggtgg tgcagcataa
    18061 tctgcaaacc gttctcggtt atgacttgtt agccggtcag cgagtgttgc gggagcagat
    18121 tgcccgcctg atgctcgaca gcggctcggt ggtcaccgcc gatgacatca tcatcaccag
    18181 cggctgccat aactcgatgt cgctggcgtt aatggcggtg tgtaaaccgg gcgatattgt
    18241 cgcggtcgaa tctccctgtt attacggttc gatgcagatg ctgcgaggca tgggcgtgaa
    18301 agtgattgaa atcccaaccg atccagaaac tggcatcagc gttgaagcac tggaactggc
    18361 gctggaacag tggccgatta aaggcatcat tctggtgcca aactgtaata atccgctggg
    18421 atttattatg ccggacgcac gcaaacgggc cgttctctct ctcgctcagc gtcatgatat
    18481 tgtgattttt gaagatgatg tctacggcga actggcaacg gagtatccgc gcccgcggac
    18541 catccattca tgggatatcg acgggcgagt gctgttgtgc agctcgttca gtaaaagtat
    18601 tgctccaggc ctgcgcgtgg gttgggtcgc accggggcgt tatcacgata aactgatgca
    18661 tatgaaatac gccatcagca gctttaatgt gccgtccacg caaatggcgg cggcaacgtt
    18721 tgtgttggaa ggccactatc atcgccatat ccggcggatg cggcagactt atcagcgcaa
    18781 tctggcgctt tatacctgct ggatacggga atattttccc tgcgaaatct gtattacgcg
    18841 cccgaaaggc ggatttttac tgtggataga attgcctgaa caggtcgata tggtctgcgt
    18901 tgcgcggcag ctgtgccgca tgaatatcca ggtggcagca ggctcgattt tctcggcttc
    18961 cggcaaatac cgtaattgtc tacgcatcaa ctgcgctttg ccgctcagcg aaacctatcg
    19021 cgaagcacta aagcaaattg gcgaggccgt gtatcgggca atggaataaa ttcgaattag
    19081 tggcgaagca cctgatttcc tctcacgaat cgcctgtaac tcgttgtatc tttgcagggt
    19141 catcgccacg ttccagcatg gcttaggcaa agtgtgcttc gttcataccg gatgcggcgt
    19201 gaacgcctta tccggcctac aaaccgcgca aattcaatag attgcagagg aaatgtaggt
    19261 ctgataagcg tagcgcatca ggcagctttg cgtttgtcat ccgtctctga ttagtattcg
    19321 atccatggat cgcgcttgat ttcaggcaag ggctgctcta atgcctgcat ttgctctgtc
    19381 gaaagtccgg taatcttttg tatccattca ggatctgccc cattctgcaa caacaatcta
    19441 aggaggcgtt gttccccttc ttctttcccc ttttccatcc ccaacagcca tccatcatta
    19501 taaattcgct ctgcaatcgt cattattcgc tccctgtgtt gtggcattcg acgggtaagt
    19561 tcgctgataa acgccttaaa acgcgcttca tcgccagtca gtaaaatgta atttaacagc
    19621 gcagttatct ggctgtcatt agcacactct gtaaccagta atactaccag ttggtcaata
    19681 agccccatca gatcgcgctg gcgaatatgc ttttggatca actccaacag ggcgactctg
    19741 cgatgctgca caatctcgtc gtctggcacg acagtgacat ccaccagcgg gaacgctgcg
    19801 ctataaagtt tccgtgcggt agtcggggcg gcaaattcgt ccagccagca cagggaccag
    19861 gggtaaggac tacggctacc gtgataaaac agcatcggga taaccagcgg tagcggccgg
    19921 cgtttatcat gctcgatatg gcgctgcatc accgccatgg aatatcgcat caggcgaaac
    19981 gccatatgaa tatcctcgcg gctctgatgt tcaatcacta cgtaaatata accatcacct
    20041 tcacgggtct ttaccgacca cagaatatcg gagtgtagcg cccgcaattt ttcatcgaca
    20101 aagctggcgg attccagttt taagctgtcg agatcgcaca gttcacgtaa atctttaggt
    20161 aagtggatct ccataaaatc ccgcgcggtg ccagggtgcg tgagaaagga cttaaataat
    20221 gcgtcatgcg gcgtgctggt cgagaagttt gtcatggcga tccgtcacct gtagataaga
    20281 tggcccgact ttagcggcta acaaaaccat tctcacccgg caatttcccg ttccccgagg
    20341 cgctttccag gctattttta ttcttctgca aacgatacat tttttcggaa acaggctcgc
    20401 gttttacatt tcctgtaccc ggcagatatt cccctttcca ccgcgctctt ttgattaacc
    20461 gcaaatttta attacactta aggtgtatat tttctatgca acccatcaat taaagaggtg
    20521 taatgtgctg atgactattt gtaatcgtta tacattctga cctggagtca gaaggtattt
    20581 ctctgtctgt gtgttcacag gcagtgtggt tgattacatg aaattcagta catttgtcct
    20641 tctcgcctgc ctttcgtcat taccgacggt attgaatttc gttttccccg ttggggttct
    20701 ccggacaagg agttgtttgt tatgccacgt ttttttgccc gccatgccgc cacgctgttt
    20761 ttcccgatgg cgttgatttt gtatgacttt gccgcgtacc tgtcgacgga tctgatccag
    20821 ccggggatca ttaatgtggt cggtgatttt aatgccgatg tcagtctggc cccggcagcc
    20881 gtcagtctct atctcgcggg cggtatggcg ttacagtggc tgctggggcc gctttccgac
    20941 aggattggcc gcaggccggt gctgattacc ggggcgctaa tatttaccct tgcctgcgct
    21001 gcgacaatgt tcacaacgtc tatgacacag tttcttatcg cgcgtgcaat tcagggcacc
    21061 agtatctgtt ttattgccac cgttggttat gtcacggtgc aggaggcgtt cggccagaca
    21121 aaagggatca agttgatggc gattatcacc tccatcgtac tgattgcgcc gattattggc
    21181 ccgctttccg gcgcagctct gatgcacttt gtgcactgga aagtcctttt tgccatcatt
    21241 gcagttatgg gttttatctc atttgttggc ttactgctgg cgatgccaga gacggtgaag
    21301 cgcggcgcgg ttccatttag cgccaaaagc gtctttcgcg attttcgtaa tgtcttttgc
    21361 aatcggctgt tcctctttgg cgcagcaacc atctctttaa gctatatccc aatgatgagt
    21421 tgggtggctg tctcgccggt gatcctgatc gacgcgggcg gcttaacaac ttcgcagttc
    21481 gcctggacac aggttccggt gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt
    21541 ttcgttaaag atccgaccga atcgcgcttt atctggcgtg ctgtacccat tcaactggtc
    21601 ggcctcgcac tgttgattat cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg
    21661 ctgggcacca gtttgtatgc tttcgggatt ggtttgatct tcccgacctt attccgcttt
    21721 acgctttttt ccaataactt accgaaaggg accgtctccg catcgctgaa tatggtgatc
    21781 ctgatggtga tgtctgtctc ggtcgaaatc ggccgctggc tgtggtttaa cggcggtcgc
    21841 ttgccgtttc atctgttagc cgtcgtggcg ggcgttatcg tcgttttcac cctggcagga
    21901 ttgcttaatc gcgtgcgcca acatcaggct gccgagctgg cggaggagcg gtgatttttg
    21961 cgcgatcctg ccgtcaggct ctattcttaa cgttatgaat aaactcattg aactcagacg
    22021 cgccaaaatg ttggcgctct ctttactgct tatcgccgct gctacctttg tcgttacgct
    22081 gtttttgccg cccaattttt gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt
    22141 cggcgcgctg gcggactggt ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat
    22201 catttctcgt catacggcga ttatcccgcg taataaagac cggattggcg aaaatctcgg
    22261 ccagttcgtg caggaaaaat ttctcgatac ccagtcgctg gtggcattga ttcgacgtca
    22321 cgaaccggcg ttgctgattg gcaactggtt tagccagcca gaaaacgccc gccgcgttgg
    22381 tcagcatctg ttgcagatca tgagtggttt tcttgaactg accgatgatg cgcgtattca
    22441 gcgcctgctt aagcgcgcgg tccatcgggc gattgataag gtcgatcttt ccggaaccag
    22501 tgcgttgatg ctggagagta tgaccaaaaa cgatcgtcat caggtgctgc tcgatacgct
    22561 gatcgcacag ttgatcgccc ttctccagcg cgataaatcg cgcaagttta ttgcccagca
    22621 gattgttcgc tggctggaga gtgagcatcc actgaaagcc aaaattctcc ccaccgaatg
    22681 gttgggcgaa catagcgcgg agttggtttc tgacgcggtg aattctttac ttgatgatat
    22741 cagccgtgat cgtgcgcatc agatccgtca tgcgtttgat cgcgccactt ttgccctgat
    22801 cgacaagttg aaaaacgatc cggaaatggc agcgcgagcc gatgccgtaa aaagttatct
    22861 gaaagaagat gaagctttta accgctatct cagtgaattg tggggggatt tacgggagtg
    22921 gctgaaagcg gatatcaaca gtgaagattc tcgtgtgaaa gaacgtatcg cgcgggcggg
    22981 tcaatggttt ggcgaaacgt taattgccga tgatgccttg cgggcgtcgt taaatggtca
    23041 cctggaacaa gccgcgcacc gcgtcgcgcc tgagttttcc gcattcctga cgcgccacat
    23101 cagcgataca gtaaaaagct gggatgcgcg ggatatgtcg cggcaaatcg agttaaatat
    23161 cggcaaagat ctgcagttta tccgtgtcaa cggtacgctg gttggcggtt gtattgggct
    23221 aattttatat ttgttgtcgc agctcccggc cttgttcccc ctcggcaatt tttagaaatc
    23281 catgaacaac taagtggcta accgcgagag agatcaaata aaccacacta aaagaatggt
    23341 aataatgtgg cctcttttca ttatctcccg tggtacgggg aaggaaaatc atgtcactta
    23401 tcaccgatct acccgccatt ttcgatcagt tctctgaagc tcgccagaaa ggctttctca
    23461 ccgtcatgga tctcaaggag cgcggcattc cgctggttgg cacttactgc acctttatgc
    23521 cgcaagagat cccgatggca gccggtgcgg ttgtggtttc gctctgttct acctctgatg
    23581 aaaccattga agaagcggag aaagatctgc cgcgcaacct ctgcccgctg attaaaagca
    23641 gctacggctt cggcaaaacc gataaatgcc cctacttcta cttttcggat ctggtggtcg
    23701 gtgaaaccac ctgcgacggc aaaaagaaaa tgtatgaata catggcggag tttaagcccg
    23761 ttcatgtgat gcagttgccg aacagcgtta aggacgatgc ctcgcgtgcg ttatggaaag
    23821 ccgagatgct gcgcttgcaa aaaacggtag aagaacgttt tgggcacgag attagcgaag
    23881 atgctctgcg cgatgccatt gcgctgaaaa accgcgaacg tcgcgcactg gctaattttt
    23941 atcatcttgg gcagttaaat cctccggcgc ttagcggcag cgacattctg aaagtggtct
    24001 acggcgcaac cttccggttc gataaagagg cgttgatcaa tgaactggac gcgatgacag
    24061 cccgcgttcg tcagcagtgg gaagaaggcc agcggctggc cctgcgtcca cgcattttaa
    24121 tcaccggctg cccgattggc ggcgcagcag aaaaagtggt gcgcgcgatt gaagagaatg
    24181 gcggctgggt tgtcggttat gaaaactgca ccggggcgaa agcgaccgag caatgcgtgg
    24241 cagaaacggg cgatgtctac gacgcactgg cggataaata tctggcgatt ggctgctcct
    24301 gtgtttcgcc gaacgatcag cgcctgcaaa tgctcagcca gatggtggaa gaatatcagg
    24361 tcgatggcgt agttgatgtg attttgcagg cgtgccatac ctacgcggtg gaatcgctgg
    24421 cgattaaacg tcatgtgcgt cagcagcaca acattcccta tatcgctatt gaaacagact
    24481 actccacctc agatgtcggg cagctcagta cccgtgtcgc ggcctttatt gagatgctgt
    24541 aaggagtggc agtgacatat tcgattggca ttgattctgg ttctaccgcc accaaaggga
    24601 tcttactggc agacggcgtg attacgcgcc gtttcctcgt tccaacaccc tttcgtccgg
    24661 caacagcaat tactgaagcc tgggaaactc tgcgcgaagg gttagagaca acgccgtttc
    24721 tgacgctcac cggctatgga cggcaactgg tggattttgc cgataaacaa gtgacggaaa
    24781 tctcctgtca tgggctgggc gcacggtttc ttgcgccagc aacgcgcgcg gtaatcgaca
    24841 tcggtggtca ggacagcaaa gtgattcagc ttgatgatga cggtaacctg tgcgatttcc
    24901 tgatgaatga caaatgcgcg gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc
    24961 ttggcaccag cgtcgagcaa ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca
    25021 cgagtatgtg cacagtgttt gccgaatcag aagtgatcag cctgcgctca gcgggcgtcg
    25081 cgccagaagc gattctcgcc ggagtgatta acgcgatggc gcggaggagt gccaatttca
    25141 ttgctcgtct ctcctgtgaa gcgccgattc tgtttactgg tggcgttagt cattgccaga
    25201 cgtttgcccg gatgctggaa tctcatctgg gaatgccggt aaatacccat cctgatgcgc
    25261 aatttgctgg cgcaattggc gcggcggtaa ttggtcaacg agtgaggaca cgccgatgaa
    25321 agagttttta tttttatttc actccacggt cggcgtcata caaacccgca aggcgttgca
    25381 ggcagcgggc atgacttttc gcgtcagcga tattccccgt gatttacgcg gcggctgcgg
    25441 gttatgcatt tggctgacct gtccccccgg cgaggaaata caatgggtga tccctgggca
    25501 gactgagtca atttattgcc agcaggatgg tgtctggcgc tgcattgcac attacggggt
    25561 ttctccgcgt taaggattca ttaaggtcgc tacgtatact tctcgatatc ttattgattt
    25621 gccttaaagg gattaccgtg acaaaaagta tttcgttaag taaacgcatc tccgttattg
    25681 tgattctttt tgccattgtg gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa
    25741 gcaatcatgc agcatcattt cagaattacc acgccaccat tgacggcaaa gaaattgcag
    25801 gcataacgaa taatatctct tcgctgacct ggtcggcaca aagcaatact ctttttagca
    25861 ctatcaacaa acctgccgcc attgttgaaa tgaccaccaa tggtgatttt atccgcacaa
    25921 ttcctctgga ttttgttaag gatctcgaga cgatcgaata cattggcgat aatcagtttg
    25981 tcattagcga tgagcgcgat tacgccattt atgtgatttc actgacccca aactcggaag
    26041 taaaaatcct caaaaaaata aaaatcccgc tgcaagactc cccaaccaat tgcggttttg
    26101 aagggttggc ttattctcgc caggatcaca ctttctggtt ttttaaagag aaaaatccga
    26161 ttgaggtcta caaagttaac gggctgttaa gtagcaatga attacacatc agcaaagacg
    26221 aggcactaca acggcaattt actttagatg atgtgtccgg cgcagaattt aaccagcaaa
    26281 aaaatacgct tctggtgcta tctcatgaat ctcgcgcact ccaggaagta accctggttg
    26341 gcgaggttat cggtgaaata tcactaacca aaggtagtcg aggtttgtcg cataacatca
    26401 aacaggccga agggattgcg atggatgcct ctggtaatat ttatatcgtt agcgaaccaa
    26461 atcgttttta tcgctttacc cctaaatcat cgcattaaca ggctggcata tttcaccttt
    26521 ttgttattaa cttgttaaca acaaaaatga catacaaggt taaacatgcc ttcgtcctcg
    26581 catcccgtag aacgcttttc tttcagcacc gcgcttttcg ggatgctggt tctgacctta
    26641 ggtatgggat taggtcgctt cctttatacg cctatgttgc ccgtcatgat ggcggaaggg
    26701 gcgttttcat ttagccagct ctcgtggatt gccagcggca actatgcggg gtatctggct
    26761 ggcagtctgc tattttcgtt tggcgcattt caccagccgt cgcgcctgcg cccattactg
    26821 ttggtttctg ccctggcgag cgggttgttg atcctcgcaa tggcatggtt gccgccattt
    26881 attctggtgt tattgattcg cttcctggcg ggtgtcgcca gcgccggtat gctgattttt
    26941 ggttcgacgc tgattatgca gcacacgcgc catccttttg tgctggcagc tttgttttct
    27001 ggcgttggca ttggcattgc actgggcaac gaatatgttc tggcaggcct gcattttgcc
    27061 ttctcctcgc aaacgttatg gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca
    27121 cttacgcttt taatgccctc gaaaaaacac gccatcgcac caatgccatt ggcaaaaacg
    27181 gagcaacaga taatgagctg gtggttactg gctattctgt acggccttgc gggatttggt
    27241 tatatcatcg tcgcgactta cctgccgctc atggcgaaag atgcaggctc accattgtta
    27301 accgcccatc tctggacgtt agtcggctta tcaattgtgc caggctgctt tggctggcta
    27361 tgggcagcaa aacgttgggg ggctttgcct tgcctgacgg cgaatttgct ggtgcaggct
    27421 atcagtgtgc tgcttactct cgccagcgac tctcctctct tgcttatcat tagtagtatc
    27481 ggttttggcg gtacctttat gggcacgaca tctctggtga tgactatcgc ccgccaactg
    27541 agcgttccgg gaaatctcaa tcttttaggc tttgtgacgc tcatttacgg cattggacaa
    27601 atcctcggcc cggcgctgac cagtatgctc ggcaatggaa catcggcgct tgccggcgcc
    27661 actctctgcg gcgcggcggc gttgtttatt gctgcgctta tcagcacagt acaattattt
    27721 aagctgcaag tggtcacttc atgaggagtg tttcccttac cactcaagaa actcaacagc
    27781 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
    27841 catttcctgt gctttgacgg ttaagataac cggcgatccg tgtcttgctc ccactttgcg
    27901 tgccgttgct tcatcagcag agagatgtac gtaatgacgc tcccctgcaa tcagcccctg
    27961 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
    28021 tttttcggca aaggaaatgg cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca
    28081 cctaccatca ctggaataac taaaacgctt tttatcgctg gtggcaacca cagtatccag
    28141 caatgcacgg gttaaccgct tgcccgcttt ttgggcacaa agtatgagct tatcgatatc
    28201 cgcccatcct tcacggtcaa gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
    28261 acttaaaaac ttgctcgtat ccgccaactc tttttcgttg tactttgcca tcgcgttttc
    28321 gtccctggat tttctgtttt tatttactga tgtcattgtc actgggttta tgaattttct
    28381 gcaactcaat cctgcgcaga gccttttcta cacagcgtaa tttttccgct atgcaaaaaa
    28441 cgcatagcaa gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
    28501 gctccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt
    28561 atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa
    28621 gggtatctgg ctttttttct caccataatc tttttttccg gagttttttc cgggacagac
    28681 agctggtggc gcgtttttga tttttccgtt ctgaacggat cgtttgggca actgcctggt
    28741 gcgaatggag ccaccacctc atttcgcggg gcgggtggcg cgggggctaa agatggcttt
    28801 ctctttgcgc tggagctggc accgtcagtc attctctctc tggggattat ttcaattacc
    28861 gatggtcttg gtggattacg cgccgcgcag cagttaatga cgcctgtgct aaaaccgttg
    28921 ttgggcattc cagggatctg ctcgctggcg ctgatcgcta acctgcaaaa cactgatgcc
    28981 gccgccggta tgaccaaaga gctggcgcag gaaggggaaa tgaccgaacg cgataaagtg
    29041 attttcgccg catatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc
    29101 gtcgcggtgt tcgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg
    29161 tttaaatttg tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac
    29221 ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg gatatgttta
    29281 tcgatggcgc gcgccgtggc tttactatcg ccacgaccaa cctgctgcca aacgtggtga
    29341 tggcgtttgt catcattcag gcgctgaaaa ttaccggcct gctcgactgg gtggggcata
    29401 tctgtgaacc cgtaatggcg ctgtggggat tacccggtga agcggcaacc gtccttttag
    29461 ccgcattaat gagtatgggc ggcgcagtcg gtgttgccgc cagtctggcg accgccggcg
    29521 cattaaccgg tcacgatgtc accgttttac tgcccgcgat gtatttaata ggcaacccgg
    29581 tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat tacccgcata
    29641 tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt
    29701 aacaaggagt taccatgatt gattataccg cagccggttt taccctgctg cagggagcgc
    29761 atttgtatgc gccggaagat cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta
    29821 tcgccgttgc cagcaatatc ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca
    29881 gcgggcagat cctctgtcct ggttttattg atcaacacgt ccatttgatt ggtggtggcg
    29941 gcgaagcagg tcccacgacg cgcacgccgg aagtggcgct aagtcgcctg acggaagcgg
    30001 gcgtcacgtc agtggttggt ctgctgggca ccgactctat ctctcgccac ccggaatccc
    30061 tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg
    30121 cttatcatgt cccttcccgc accattacgg gttccgtgga aaaagacgtg gcgattatcg
    30181 atcgtgtgat tggcgtgaaa tgcgccatct ctgatcaccg ttctgccgca ccggacgttt
    30241 atcacctggc caatatggcg gcagaatccc gcgttggcgg tttgctcggc ggtaaacctg
    30301 gcgtcaccgt gttccacatg ggcgacagta aaaaggcgtt acagcctatt tatgacctgc
    30361 tggaaaactg cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgtac
    30421 cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgat atcaccagca
    30481 gcattgacga accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc
    30541 cgctggcacg cgtcaccctc agctccgacg gcaacggtag ccagccgttc ttcgatgacg
    30601 aagggaattt aacccatatc ggtgttgcag gttttgaaac gttgctggaa accgtgcagg
    30661 tgctggtcaa agactatgat ttcagtatca gcgatgccct gcgcccgctc accagtagcg
    30721 tagccggttt ccttaacctg accgggaaag gtgaaattct gccaggcaat gatgctgact
    30781 tgctggtcat gacgccagaa ctgcgcattg agcaggtata cgctcgcggc aaactgatgg
    30841 tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc
    30901 gcccgaatgg acgtagaatg cccgcaaggg cggctgacag agtaaaacgt aatggatgac
    30961 tgtggtgcga ttttgcataa tattgaaacc aaatggcttt atgattttct gaccctggaa
    31021 aaatgccgca atttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc
    31081 cgccgcatcc gtgcgctgga acaggcgatt ggtgttgaat tgtttaaccg ccaggtaacg
    31141 ccgctgcaac tctcagaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa
    31201 cagttagaga gcaatctggc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc
    31261 aagatagccg ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg
    31321 ccgccgctct ttacctgggc aattgaagcc attgatgtcg atgaagcggt cgataaactg
    31381 cgtgaagggc aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg
    31441 tttgaccaca ttcgcttatt tgaatctcaa ttgttccctg tctgcgccag tgacgaacac
    31501 ggagaagcac tttttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac
    31561 tcctacatgg ggcgattgat taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc
    31621 accttttttg tctcttcgat gagcgagctt ttaaagcagg ttgccctcga cggctgtggg
    31681 attgcctggc tgccggagta cgccatccaa caagaaattc gcagcgggaa gctcgttgta
    31741 ctgaatcggg acgaactggt gatcccgatt caggcctatg cataccggat gaatacccgc
    31801 atgaatcccg ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc
    31861 tgacattctc cagcgtcgca ctgcgccaac cgccattatc ccagaccaga tcaaacgtta
    31921 gcggttcatc gtcgtggtac agcattatct caaggctgat cgccagagtg tggcagtacg
    31981 cttctttcag attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac
    32041 gcagctgacg gcaataccag ctctggagat gtttgtcgct cgggttatgc acttgccact
    32101 cgggcatgcc tgaaacaagt aatgacggta atgccgaatg aggagaatta aaaagagtga
    32161 ggagatcgct gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta
    32221 aaacagcctg aagtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc
    32281 ggcttgccct gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt
    32341 ttgttcatta catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa
    32401 ccttacttgc ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa
    32461 tcatcaaact caaaaaaaaa ccgctagtat attctgaata ttccgaatag ttatgtagat
    32521 gaaaatactt gtcaccgccg ctacttaatt ccggtgggaa attatttatt tccgcataat
    32581 cagc
//
LOCUS       DAZFWP020000040        32421 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFWP020000040 DAZFWP020000000
VERSION     DAZFWP020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.040"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..118
                     /locus_tag="ACHK0Z_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             <1..118
                     /locus_tag="ACHK0Z_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4501776.1"
                     /translation="RTAIPFEGERHNALDDARYQAKYVSAIWKKLIPSQADF"
     gene            190..441
                     /locus_tag="ACHK0Z_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             190..441
                     /locus_tag="ACHK0Z_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4501777.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            461..1756
                     /locus_tag="ACHK0Z_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             461..1756
                     /locus_tag="ACHK0Z_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGU4501778.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLANNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLINPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVTLLNTQLSEFAPTMPHNEAMKS"
     gene            complement(1944..2963)
                     /gene="rspB"
                     /locus_tag="ACHK0Z_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             complement(1944..2963)
                     /gene="rspB"
                     /locus_tag="ACHK0Z_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HGU4501779.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2975..4189)
                     /gene="rspA"
                     /locus_tag="ACHK0Z_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             complement(2975..4189)
                     /gene="rspA"
                     /locus_tag="ACHK0Z_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGU4501780.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4395..4721)
                     /gene="ynfA"
                     /locus_tag="ACHK0Z_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             complement(4395..4721)
                     /gene="ynfA"
                     /locus_tag="ACHK0Z_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU4501781.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4856..5197
                     /gene="ynfB"
                     /locus_tag="ACHK0Z_RS08735"
                     /old_locus_tag="pgaptmp_001747"
     CDS             4856..5197
                     /gene="ynfB"
                     /locus_tag="ACHK0Z_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU4501782.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5232..5792
                     /gene="speG"
                     /locus_tag="ACHK0Z_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             5232..5792
                     /gene="speG"
                     /locus_tag="ACHK0Z_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU4501783.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5795..6505)
                     /gene="ynfC"
                     /locus_tag="ACHK0Z_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             complement(5795..6505)
                     /gene="ynfC"
                     /locus_tag="ACHK0Z_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU4501784.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VNPAVERVYTIKNTIDYY"
     gene            6613..6918
                     /gene="ynfD"
                     /locus_tag="ACHK0Z_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     CDS             6613..6918
                     /gene="ynfD"
                     /locus_tag="ACHK0Z_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU4501785.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            7117..9543
                     /gene="ynfE"
                     /locus_tag="ACHK0Z_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             7117..9543
                     /gene="ynfE"
                     /locus_tag="ACHK0Z_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HGU4501786.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            9604..12027
                     /gene="ynfF"
                     /locus_tag="ACHK0Z_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             9604..12027
                     /gene="ynfF"
                     /locus_tag="ACHK0Z_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU4501787.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            12038..12655
                     /gene="ynfG"
                     /locus_tag="ACHK0Z_RS08765"
                     /old_locus_tag="pgaptmp_001753"
     CDS             12038..12655
                     /gene="ynfG"
                     /locus_tag="ACHK0Z_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU4501788.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12657..13511
                     /gene="ynfH"
                     /locus_tag="ACHK0Z_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             12657..13511
                     /gene="ynfH"
                     /locus_tag="ACHK0Z_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU4501789.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13554..14168
                     /gene="dmsD"
                     /locus_tag="ACHK0Z_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             13554..14168
                     /gene="dmsD"
                     /locus_tag="ACHK0Z_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU4501790.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14327..15619
                     /gene="clcB"
                     /locus_tag="ACHK0Z_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             14327..15619
                     /gene="clcB"
                     /locus_tag="ACHK0Z_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU4501791.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15572..16267)
                     /gene="bioD"
                     /locus_tag="ACHK0Z_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             complement(15572..16267)
                     /gene="bioD"
                     /locus_tag="ACHK0Z_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4501792.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16392..17612)
                     /gene="mlc"
                     /locus_tag="ACHK0Z_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /gene_synonym="dgsA"
     CDS             complement(16392..17612)
                     /gene="mlc"
                     /locus_tag="ACHK0Z_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU4501793.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLLGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17747..18640)
                     /gene="ynfL"
                     /locus_tag="ACHK0Z_RS08795"
                     /old_locus_tag="pgaptmp_001759"
     CDS             complement(17747..18640)
                     /gene="ynfL"
                     /locus_tag="ACHK0Z_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4501794.1"
                     /translation="MNIELRHLRYFVAIAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYEDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18747..20000
                     /gene="ynfM"
                     /locus_tag="ACHK0Z_RS08800"
                     /old_locus_tag="pgaptmp_001760"
     CDS             18747..20000
                     /gene="ynfM"
                     /locus_tag="ACHK0Z_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4501795.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(20049..20168)
                     /locus_tag="ACHK0Z_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             complement(20049..20168)
                     /locus_tag="ACHK0Z_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU4501796.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20424..20732
                     /gene="asr"
                     /locus_tag="ACHK0Z_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             20424..20732
                     /gene="asr"
                     /locus_tag="ACHK0Z_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU4501797.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETANTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            20825..20908
                     /locus_tag="ACHK0Z_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             20825..20908
                     /locus_tag="ACHK0Z_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501798.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            21008..21829
                     /gene="ydgD"
                     /locus_tag="ACHK0Z_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             21008..21829
                     /gene="ydgD"
                     /locus_tag="ACHK0Z_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU4501799.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21868..22197)
                     /gene="mdtI"
                     /locus_tag="ACHK0Z_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             complement(21868..22197)
                     /gene="mdtI"
                     /locus_tag="ACHK0Z_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU4501800.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(22184..22549)
                     /gene="mdtJ"
                     /locus_tag="ACHK0Z_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     CDS             complement(22184..22549)
                     /gene="mdtJ"
                     /locus_tag="ACHK0Z_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU4501801.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVMLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            22961..23104
                     /locus_tag="ACHK0Z_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /pseudo
     CDS             22961..23104
                     /locus_tag="ACHK0Z_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(23615..23896)
                     /locus_tag="ACHK0Z_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             complement(23615..23896)
                     /locus_tag="ACHK0Z_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501802.1"
                     /translation="MTEAEILRLIRRVSGISQQHDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYRRRVQTYREKAKITYHWKKPERLRT"
     gene            complement(23910..25571)
                     /locus_tag="ACHK0Z_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             complement(23910..25571)
                     /locus_tag="ACHK0Z_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4501803.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLENSLYMFDREV
                     VERFIVFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPEMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGK
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWARAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(25555..25911)
                     /locus_tag="ACHK0Z_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             complement(25555..25911)
                     /locus_tag="ACHK0Z_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGU4501804.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(26035..26217)
                     /locus_tag="ACHK0Z_RS08855"
                     /old_locus_tag="pgaptmp_001771"
     CDS             complement(26035..26217)
                     /locus_tag="ACHK0Z_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501805.1"
                     /translation="MALMNEREQNRLIRGLIRQRDAWKAQEAGHKDKASGRAERITAT
                     RLNDRDREVMECFRNR"
     gene            complement(26201..26641)
                     /locus_tag="ACHK0Z_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             complement(26201..26641)
                     /locus_tag="ACHK0Z_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4501806.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRSARQQQDSRRGSSRER
                     GYTRQWEKYRAMYLSKNPLCAHCLEKGIYTPAVVVDHIIPIDGGNDVLFWPEWNHQPL
                     CQACHNQKTKWLDPSTKNKRAAGGFREEEERGANRNNWMYGADE"
     gene            complement(26641..26934)
                     /locus_tag="ACHK0Z_RS08865"
                     /old_locus_tag="pgaptmp_001773"
     CDS             complement(26641..26934)
                     /locus_tag="ACHK0Z_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4501807.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTHVPMTIGALWTAYRDVGVY"
     gene            complement(26931..27269)
                     /locus_tag="ACHK0Z_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             complement(26931..27269)
                     /locus_tag="ACHK0Z_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4501808.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWVDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCSEGAEK"
     gene            complement(27266..28477)
                     /locus_tag="ACHK0Z_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             complement(27266..28477)
                     /locus_tag="ACHK0Z_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4501809.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(28479..29036)
                     /locus_tag="ACHK0Z_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             complement(28479..29036)
                     /locus_tag="ACHK0Z_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4501810.1"
                     /translation="MKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYEKFQRG
                     AFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDDTGLRFELTPPDTSTGRDVIE
                     LVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGVELARR
                     SLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(29092..30249)
                     /locus_tag="ACHK0Z_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             complement(29092..30249)
                     /locus_tag="ACHK0Z_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4501811.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(30541..30765)
                     /locus_tag="ACHK0Z_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     CDS             complement(30541..30765)
                     /locus_tag="ACHK0Z_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501812.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNINDYRAFLETGALIEKDRHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            complement(30891..31163)
                     /locus_tag="ACHK0Z_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             complement(30891..31163)
                     /locus_tag="ACHK0Z_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4501813.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMMLVETDKERHHITMRRL
                     ECSRKAQRPPEPPDNFGDLRNAVNRTCAEMGIDGAGDEIRRNYQDS"
     gene            complement(31174..31584)
                     /locus_tag="ACHK0Z_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             complement(31174..31584)
                     /locus_tag="ACHK0Z_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4501814.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADQLAGHEKGNVISVSGTMQISQWTGQNGETRQGYQVIADSVISA
                     KTVRPGGSRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            complement(31594..31899)
                     /locus_tag="ACHK0Z_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             complement(31594..31899)
                     /locus_tag="ACHK0Z_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501815.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(31896..32147)
                     /locus_tag="ACHK0Z_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             complement(31896..32147)
                     /locus_tag="ACHK0Z_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501816.1"
                     /translation="MTKMRRDRTQQKYKALDMTELSLKVAIKVIDRHTREGYAKENPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
BASE COUNT         7895 a         8385 c         7804 g         8337 t
ORIGIN      
        1 cagaactgct atcccattcg aaggtgagcg ccataatgca cttgatgacg cccgttatca
       61 ggcaaaatac gtttcagcta tctggaaaaa actgatcccg agtcaggctg atttttaatg
      121 ttcaaccgtc gtcggttgca attggtattc tgcaaccggc gttcatctga tgtaagagat
      181 aaagaatcga tgagcgaagt aatcatgatc gtctctcctg gcaaatgggt atccgaagag
      241 cagttaattg cacttaaagg aatcaaaaag ggaacgctaa aaaaagccag ggaaaaatcg
      301 tttatggaag gaagggaata taagcatgtc gctcatgacg gtatgccatg ggataacagt
      361 ccatgctttt acaacctgga agaaattgat cgctggattg agcgccaggc atctgcaaga
      421 ccaagacgtc atcttgcttg actaaaagca acacgaacca atgagagaag ctgaaatgaa
      481 atatccgaca ggcgtggaaa accatggagg gaaattacgt atctggtttg tttataaagg
      541 tgtaagagtc cgggaaaatc ttggggttcc tgacacagca aaaaacaggc gcattgcagg
      601 tgagctacgc gcctctgttt gttacgcaat aaaaactggc gctttcgact atgcaaaaca
      661 gtttcccgcc tcacacaatc tggaaaaatt tggtgaggcc cgacaagatt taaccataaa
      721 agaactggct gaaaaatttc tggcactgaa agaaactgaa gtcgcaaaaa cgtcactcaa
      781 cacgtaccgt gccgtcatca aaaatattct gagcataatc ggtgaaaaaa atcttgcatc
      841 atcgattaat aaagaaaaat tgctggcggt acgtaaagag ttactgactg gataccagat
      901 ccccaaaagt aactatattg ttacacaacc agggagatcg gctgttaccg taaataatta
      961 catgacaaat ctttacgccg tgttccagtt tggtgttgat aacggttatc tggcaaacaa
     1021 tccatttaag gggatctcac cattaaagga gtcgagaacc atcccggatc cactttcgcg
     1081 ggaggagttt atccgcctta ttgacgcgtg cagaaatcag caagccaaaa atttatggtg
     1141 tgtttccgtt tatactggga ttcgccctgg tgaactgtgt gcgcttggat gggaagatat
     1201 agatctgaaa aatggaacaa tgataatcag aagaaatctg gcaaaagacc gttttacagt
     1261 accaaaaaca caggcgggaa ccaatcgtgt gattcacctt attaatcccg caatcgacgc
     1321 tctccggagt cagatggcac taacgagact gagcaaagag catatcatcg atgttcacct
     1381 cagagaattc ggcagaacag agaaacaaaa atgtaccttt gtttttcaac ctgaagtgtc
     1441 agcgaaagta aaaaattatg gtgaccattt taccgttgac tcaataaggc agatgtggga
     1501 cgcagcggta aagcgtgccg gaatccgcca tcgcaaatcg tatcagtcga gacacactta
     1561 tgcctgctgg tcgttaacag caggagctaa cccggcattc attgcaaacc agatgggcca
     1621 tgcagatgcg caaatggtgt ttcaggtgta cgggaaatgg atgtcagaaa ataataatgc
     1681 gcaggtaacg ctgttaaata cccagttaag cgagtttgcc ccaaccatgc cccataacga
     1741 agcgatgaaa agttaatttt ttatttatca attagttaga ttgaatgact cttgaaatcc
     1801 ataaattcaa gcgcagtgcc cagccatccc gatactgctg ctttcaccaa atccttagtg
     1861 cttctttcgt gtttttctat tgtcataatg gttatctcta aaaaagaggt aagatgcgta
     1921 ctacttactc gccgttattg gtattattca gaaaaagtga gtaagacttt gcagcaatgt
     1981 ttttgatcct gttcaaataa actaatggca tcagcaacat gctggaaatc aaacgtatgg
     2041 gtaattaatt tttctggttt aattaaccct ttacttaacc agtcgataac gaccgggaat
     2101 ttatttgcat ttaagcgtga agaaaaaata gagagttctt ttccggtaat tccttgctga
     2161 atcacttcag acggttcact ggagaagccc atcaatacaa tacgtgccgc tggagaagcc
     2221 agcgttacgg cctctttcag gatagaagga tgacaagccg catcgataat taatgtcggc
     2281 ttgatgcctt tttcagtgaa aatctcgcca agcggtgtct ggctgttatt aatcgcccag
     2341 tcagccccgc tctctttcgc tttttccagt cgttcatcaa tgcgatcggc aacaatcaca
     2401 tttttaacgt tatagacgcc ttttaatacc tgaacgatcg tcaggccgat tggaccggca
     2461 ccataaacca gaacggtatc attttcagtc ggttgaccat gtccggttac gttagccgca
     2521 atggtaaaag gttcgatcat taccgcatat tgatcggcca ctgcttcagg aattttccac
     2581 gcattttttg ccggaaccac ggcatattca ctgaaaccac cgtcagcgtg cacacctaat
     2641 acagccagtg tcgtacaaac gttcggttta cctatagagc acggatagca atgcccacag
     2701 ctgaccaccg gatcgacagc aacacgttca ccgactctgg cgctttccac gccttcaccc
     2761 actgcatcaa tgacgccaaa gaattcatga ccaatgacgc gcggatattt cgcaaaagga
     2821 ttatgcccac gataaatatg gctatctgaa ccacaaattc cggcaagttt cacttttact
     2881 cgcacttcac ccgctgacgg ggtgggtatt tcacgttcga caatcgccag ttgattcggt
     2941 ttttcaatta atatgctttt cattatctta ctccttacca gttccacagc gtgccatctt
     3001 ccagacgtgc gactggtaga taagcaggtt catagggata tttcgccgcc agcttttcat
     3061 cgaattcgat accaagaccc ggtttgtctc ccggatgcat atagccgtta tcgaaagtcc
     3121 agttgtgcgg gaagacttcg agcatttgtt cggaataacc catgtattcc tggacaccga
     3181 aattggggac ccacagatca aagtgcagcg ccgcagccat gcagactggt gacaaatcgg
     3241 aaggaccgtg tgagccagta cgtacctgat acagcgaagc aaaatcggca atccggcgca
     3301 taccggtaat tccgcctgca tgggtcagcg tggtgcggat ataatcgatg agttgctctt
     3361 caatcagttg tttgcagtcc cagatgctgt tgaagacttc acccactgcg atgggtgtga
     3421 cggtatgttg gcgaatgaga cggaagcatt cctggttttc cgcaggtgtc gggtcttcca
     3481 tccagaacat gcgataatct tcaatgcttt taccaaagcg cgccgcttca ataggcgtta
     3541 agcgatggtg catgtcatgc agcaaatgtt cattaaaacc aaacttgtta cgtaccgcgt
     3601 caaacaattt cggcatgaaa tcgaggtatt tctccgtcga ccacagctgc tcttccggcc
     3661 actgtccttt ggttgcgggt tcataagcca gacctttacc tttcgacatg ccgtaggtgg
     3721 ttttcatacc agggattccg cactgcacgc ggatggcttt gaatcccagc tcctgatgac
     3781 gggcataatc atccagagct tcatcaatac tgtgaccggt ggtatggcaa taaaccatca
     3841 ccccttcacg agacgcgccg ccgagtaact ggtaaagcgg catgttggcc gctttggctt
     3901 taatatccca cagcgccata tcaaccgctg aaatggccga catcgtaacc ggaccgcgac
     3961 gccagtaagc acctttatag aaaaactgcc agatatcttc gatacggtgc gcatcgcgac
     4021 caataagctg cggacaaagg tgatcctgca aataagaggc cacggaaagc tcacgtccat
     4081 tgagagtggc atccccaagg cccgtaatac cgtcctcagt ggtgattttt aatgtgacga
     4141 aattacgccc cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct
     4201 tgcatcgctt gtcgtgatgc atgaaatcta cgcaactgag ctactaccat acaagtatga
     4261 ggatcgaaaa aagccggagt gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg
     4321 acaaaagatg aaatagaata ccttttgtca gctgacactt cctcgtatct tattgataaa
     4381 atggatttat gttcctacgt gcgcccccag cccgcaacaa tgatcaacat gccgcaaagc
     4441 gcaatcaacg cacccgtcca gtcataaaga gtcagtttca cgccatccac aacgcgcagc
     4501 cacatcaacg ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc
     4561 gcaggatgca acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac
     4621 agccagatac tggcgtttcg ttttaaccac aaccagggca gaaagcatcc aataatttca
     4681 cacagcgcag tagcaaaaaa tagtaacgtt gttttaatca tctttgtcac ttattgacat
     4741 catgtatagt tatagggcca cataatatca ccaatataaa caccctcctg atacgttttg
     4801 cgttcgcagt ggatggtgta gaatcacctt taatcattca tacagggaat gaattatgaa
     4861 aatcactctc agcaaacgaa tcggcctgct cgcttttctg ctaccttgcg cactggcatt
     4921 gagcacaact gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca
     4981 aagccgccag cacgccgcta tggaaaaaga gcaatggaat gacacgcgca atctgcgcca
     5041 gaaagtgaat aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg
     5101 cgataaatgt gagcaaagcg ccaacatcaa tgcctactgg gagccaaata ctttgcgctg
     5161 cctggaccgt cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt
     5221 aaggacacgt tatgccaagc gcccacagtg ttaagctacg cccgctggag cgtgaagatt
     5281 tacgctatgt acatcaactc gacaataacg ccagtgtgat gcgttactgg tttgaggaac
     5341 cctacgaagc ctttgttgaa ctctctgatc tgtatgataa gcatattcac gatcagagcg
     5401 aacggcgctt tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa
     5461 ttaaccatgt tcatcgccgc gcagaatttc agataattat ctccccggag tatcagggga
     5521 aaggtctggc aacccgtgcc gcaaaattag caatggacta tggctttacc gttctcaatc
     5581 tctataagct gtatctgatc gttgataaag agaatgaaaa agcgattcat atttaccgca
     5641 agcttggctt ttcggttgaa ggtgaattga tgcacgagtt ctttattaat ggtcaatatc
     5701 gtaatgccat tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg
     5761 gtcagactct cctgaagccg accgcacaat agcattaata ataatcgatc gtatttttga
     5821 tggtgtaaac ccgttcgacg gcggggttta ctccttcatc aacaatgatc agctgacagt
     5881 ccaccggatt ggcgtgactg tcatattcac agctctgttt tacattacca actcgttgat
     5941 tattcagtaa agtaactgct gtataatcta attttttgat cgggtccgtt gatggcgtgg
     6001 cgctgacaga taatgttttg tcgttacttt tcgtggtttt acccagcgga tacccctgat
     6061 catcatagcg atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat
     6121 catcggtttc ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta
     6181 aacgtactct tttctccagc gtctcagcat cacggtaata attggcgtcc agtaccagag
     6241 cgaccacggt attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg
     6301 acaaagtccc agaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga
     6361 aatcttttac cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct
     6421 caggggtaaa tgaaagtgtt acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta
     6481 agcatggtag taatttgtat ttcacaacag tcaccagaga gtagagatga ttctcaatca
     6541 tagtagcaaa tacagtactt tacacgttaa atgctatgct taaagaagtt atcttcgcgt
     6601 aaggagctta tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca
     6661 cccgcggtac tcgccgcgcc gggatcctgt gagcgcatac agagcgatat atcacagcgc
     6721 attatcaata atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag
     6781 gttgatcagc ctgattccca ggtcgtcggg cattgtgcta atgatacgca taaaattctc
     6841 tacacccgca ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggatggcgcg
     6901 cctgccgaac cgcagtaaca cattatcgac tgaacgccgg atacgacaca tccggcgatt
     6961 tgaacataca acataatccc accttattac tcataccctt ctattgatat ggattaataa
     7021 ttcttaaccc aaaatgggta gactccctct attgttagcg cgctaaatat tcaatatata
     7081 aacttttata taacgataaa gaacagggag tgagttatgt ccaaaaatga acgaatggtg
     7141 ggcatcagcc gcagaacact cgttaaatct accgcgatag gttctctggc gctggctgca
     7201 ggcggttttt ctttgccgtt taccctgcgc agtgcagcag cagcggtaca acaggcccgc
     7261 gaaaaagtgg tctggggtgc ctgttccgtc aactgtggta gccgctgtgc acttcgtcta
     7321 catgttaaag ataatgaagt gacctgggtg gaaactgaca ataccggcag cgatgagtac
     7381 ggcaaccatc aggtacgcgc ctgtttgcgc ggtcgctcca tccgtcggcg tattaatcat
     7441 cccgatcgct tgaattatcc aatgaaacgc gtgggcaaac gcggcgaagg caaattcgaa
     7501 cggattagct gggatgaagc cctggatact atcgccagta gcctgaaaaa aaccgtcgaa
     7561 caatatggca atgaggctgt atatattcag tactcttcgg ggattgttgg cggcaatatg
     7621 acccgctctt cgccatcagc ctcggcggtc aaacgcctga tgaactgcta cggcggttca
     7681 ctcaatcagt atggctccta cagcaccgcg caaatttcct gtgccatgcc ctacacctac
     7741 ggcagtaatg atggcaacag caccacggat attgaaaaca gcaagctggt cgtgatgttt
     7801 ggtaacaacc cggcagaaac ccgcatgagc ggcggtggca tcacttatct tcttgaaaaa
     7861 gcgcgcgaga aatcgaacgc caaaatgatt gttatcgatc cgcgatatac cgatacggct
     7921 gctggtcgtg aagatgaatg gcttcctatt cgcccgggca ccgatgccgc gctggttgcg
     7981 ggtattgcct gggtattgat taacgaaaat ctcgttgatc aaccttttct cgataaatac
     8041 tgcgtcggct atgacgaaaa aaccttaccc gcagatgcac caaaaaatgg tcactataaa
     8101 gcctatattc ttggtgaagg tgatgataac acagcgaaaa cgccgcagtg ggcttcgcaa
     8161 attaccggta tcccggtgga ccgtatcatc aaactggcgc gtgaaattgg cacagcaaaa
     8221 cccgcttata tctgccaggg ctgggggccg caacgccagg caaacggcga gctgactgca
     8281 cgcgctattg ctatgttgcc tattttgacg ggcaatgtcg gcatcagcgg cggaaatagt
     8341 ggcgcgcgag aatcgaccta taccattacc atagaacgcc tgccggtgct ggataatccg
     8401 gtcaaaacgt caatctcctg cttcagctgg acggatgcta tcgatcatgg tccgcaaatg
     8461 acggcaatcc gcgacggcgt ccgcggcaaa gataaactgg atgtgcccat taagttcatc
     8521 tggaactacg cgggaaatac cctggttaat cagcattctg acatcaacaa aacgcatgaa
     8581 attctgcaag acgaatcgaa atgcgaaatg attgtggtca tcgaaaactt tatgacctca
     8641 tcggcaaaat atgccgacat tctgctgcca gacctgatga ccgttgagca ggaagatatt
     8701 attcctaacg actacgccgg taacatggga tatctcattt tcctccagcc agtcaccagc
     8761 gaaaaattcg aacgtaaacc gatttactgg atcctgagtg aagtcgcgaa acgtcttgga
     8821 cctgacgtct atcaaaagtt cacagaaggt cgcacgcagg aacaatggtt acaacatctg
     8881 tacgccaaaa tgcttgccaa agatccagcg ttaccgtctt acgacgaact gaaaaaaatg
     8941 ggtatctata agcgtaaaga tcccaatggt cattttgtcg cctacaaagc atttcgtgac
     9001 gaccccgagg caaatccact taaaacgcct tccggtaaga ttgaaattta ttccagcagg
     9061 ctggcggaaa ttgcccgtac ctgggaactg gaaaaagatg aagtgataag cccattgcca
     9121 gtttatgcct caacctttga aggctgggac tcccctgaac gtagtacctt cccactgcaa
     9181 ctgtttggtt tccattacaa atcccgtact cactcgacct acggcaacat tgatgtcctg
     9241 aaggctgcct gccgtcagga ggtgtggatc aaccctatag atgcgcagaa acgtgggatt
     9301 gccaacggcg atatggtgcg ggtgtttaac catcgtggcg aagttcggct accagccaaa
     9361 gtgacaccac gcattctccc tggggttagc gctatgggcc agggagcctg gcacgaggcc
     9421 aatatgtctg gcgataaaat cgaccatggc ggctgtgtga atacgctaac cactctgcgc
     9481 ccttcaccac tggcgaaggg aaacccgcag cacactaatc tggtcgagat cgaaaaaata
     9541 taacccacta caaccataaa ttctggcatg acattttgtt tgaaaagcaa taagtgagta
     9601 atgatgaaaa tccataccac agaggcgctc atgaaggctg aaatcagccg tagaagtctg
     9661 atgaaaacct ccgcacttgg cagtcttgcg ctggcaagca gtgctttcac tctgccattt
     9721 tcccaaatgg tccgggcggc agaggctccg gtagaagaga aagcggtctg gagttcctgc
     9781 accgttaact gcgggagccg ctgtctgtta cgtttgcatg tgaaagatga caccgtgtac
     9841 tgggtggagt ctgatacgac aggtgacgac gtctacggta atcatcaggt tcgcgcgtgt
     9901 ttacgcgggc gctctatccg ccgacggatg aatcatcctg ataggttgaa atatcccatg
     9961 aagcgcgtcg gcaagcgcgg tgaaggtaaa tttgaacgga taagttggga cgaagccctg
    10021 gataccatca gtgataatct tcggcggatc ctaaaagatt acggcaatga ggctgtacat
    10081 gtcctgtacg gaacaggcgt agatggcgga aacatcacca actcaaacgt cccgtaccgt
    10141 ctgatgaact cttgcggtgg ttttctcagt cgctatggca gctacagtac cgcacagatc
    10201 agtgccgcaa tgagttatat gttcggtgcc aatgatggca acagcccgga tgatatcgcc
    10261 aatacgaaac tggtcgttat gttcggaaat aacccggcag aaacgcggat gagcggcggt
    10321 ggtgtcactt actacgtcga gcaagcccgc gaacgttcaa acgcacgcat gatcgtcatc
    10381 gatccacgtt ataacgacac agctgccggg cgtgaagatg aatggctgcc cattcgccca
    10441 ggcaccgatg gcgcactggc ctgtgcgatt gcctgggtac tgattactga aaacatggtc
    10501 gatcagccat ttctcgacaa atattgtgtt ggttacgatg aaaaaacgct gcccgccaac
    10561 gcaccacgta acgcgcatta taaagcctat attctgggcg aagggcctga cggcatagct
    10621 aaaacgccgg aatgggcagc aaaaatcacc agcatcccgg cagaaaaaat tatccagttg
    10681 gcacgagaga tcggttcagc aaaacctgct tatatttgtc agggttgggg gccacaacga
    10741 cattccaacg gcgagcaaac gtcccgcgct attgccatgc tttccgttct caccggtaac
    10801 gtcggcataa acggcggcaa ctcaggtgta cgcgaaggta gctgggatct gggggtagaa
    10861 tggttcccga tgcttgagaa ccctgttaaa acgcagattt ccgtctttac atggacagat
    10921 gccatcgacc atggtaagga aatgaccgcg acccgtgatg gtgttcgtgg aaaagaaaaa
    10981 ctggatgtcc ccatcaagtt tttatggtgc tacgccagta acacattgat caatcaacat
    11041 ggcgacatca atcacaccca tgaggtgctt caggatgaca gcaagtgcga gatgattgtt
    11101 ggcattgacc acttcatgac ggcctcggct aagtattgcg atatcctgtt gcccgacctg
    11161 atgccaacag agcaagaaga ccttatctct catgaatctg cagggaatat gggctatgtg
    11221 atcctcgccc aacccgcaac ctctgcaaaa tttgaacgca aacccatcta ctggatgctg
    11281 agtgaagtcg ccaaacgctt aggaccagac gtttatcaaa cctttactga aggtcgcagt
    11341 cagcatgaat ggatcaaata tctccatgcg aaaacgaagg aacgtaaccc tgagatgccc
    11401 gactacgagg agatgaaaac gaccgggatc tttaagaaaa aatgcccgga agaacactac
    11461 gtcgctttcc gcgcattccg tgaagatccc caggcaaacc cgttgaaaac accttcgggg
    11521 aaaatcgaaa tttattctga acgactggcg aagattgcgg atacctggga attgaaaaaa
    11581 gatgaaatta ttcatcccct tccagcttat acgccaggtt ttgatggctg ggacgatccc
    11641 ctgcggaaaa cctatccact gcagttaacg ggcttccact acaaggcgcg tacccactcc
    11701 agctacggca atattgatgt gttacagcag gcctgcccac aagaggtgtg gatcaacccc
    11761 attgatgctc aggctcgcgg tatccgtcat ggcgataccg tgcgggtatt taacaataat
    11821 ggagaaatgc tgattgccgc aaaagtgact ccgcgtattc tgcctggcgt taccgccatc
    11881 gggcaaggtg cgtggcttaa agcggatatg tttggtgacc gggtcgatca cggcggcagt
    11941 atcaatattc tgacctctca ccgcccttca ccgctggcaa agggaaaccc gtcgcacagc
    12001 aatcttgtcc agatcgaaaa ggtttaaaga gtagcccatg accacacaat atggattttt
    12061 tatcgattcc agccgctgta ctggctgtaa aacctgcgaa ctggcgtgca aagattttaa
    12121 agatcttggc ccggaagtca gtttccgccg tatttatgaa tatgctggcg gcgactggca
    12181 ggaagataat ggcgtctggc accagaacgt gttcgcttat tatctctcca tctcctgtaa
    12241 ccattgcgat gacccagcat gtacaaaagt ctgccccagc ggcgcaatgc ataagcgtga
    12301 agatggcttt gtggtggttg atgaagatgt ttgtatcggc tgccgctact gccacatggc
    12361 ttgtccgtac ggcgcaccac agtacaatgc tgaaaaaggg cacatgacga agtgcgatgg
    12421 ttgttattcg cgcgtcgccg aggggaaaca acccatatgc gtcgaatcct gcccgctgcg
    12481 tgcacttgag ttcggtccaa ttgaagagct gcgccagaag cacggcactc tggctgccgt
    12541 cgcgccactg ccgcgcgcgc atttcacaaa acccaatatc gttatcaaac ctaacgccaa
    12601 cagccgcccg accggcgata ccactggtta tctggctaat ccggaggaag tgtaaaatgg
    12661 gaaatggatg gcatgaatgg ccactggtta tctttaccgt attgggtcaa tgcgtagtag
    12721 gcgcgctgat tgttagcgga attggctggt tcgccgccaa aaatgacgca gaccgtcagc
    12781 gtattgtccg tggcatgttt ttcctctggc tgttgatggg catcgggttt attgcctctg
    12841 tcatgcacct gggatcgcca ctgcgagcgt ttaactcgct gaatcgaatc ggagcttctg
    12901 gtctgagtaa cgagattgct gccggttcca tattttttgc cgttggcgga ctatggtggc
    12961 tggtggcggt catcggtaaa atgccgcaag cgttggggaa actctggtta cttgtcagca
    13021 tggcgctcgg cgtcattttc gtctggatga tgacctgcgt gtatcaaatc gacaccgtgc
    13081 caacctggca taacggttac actacgctgg ctttcttcct gacggtattg ttgagcggtc
    13141 caatacttgc tgccgcaatt ctgcgggcag ctcgcgttac atttaatacc acaccatttg
    13201 ccatcattag cgttctggcg ctgatcgctt gtgccggggt gattgtgctg caaggtttgt
    13261 ctctggcgtg tattcactca tccgtacaac aagccagcgc actggtacca gattacgcct
    13321 ccttacaggt ctggcgtgtg gtattgttat gcgcaggtct aggatgctgg gtttgtccgc
    13381 tcattcgtcg ccgtgaacct cacgttgccg gacttattct ggggttaatc ctcattcttg
    13441 gcggtgagat gatcgggcgt gtgctctttt atggcctgca catgacggta ggaatggcta
    13501 ttgcaggtta acaaattggt gcggggcaac ccgcacattt caggatgtta ggaatgaccc
    13561 atttttcaca gcaagataat ttttctgtcg cggcgcgcgt gttgggcgcg ctgttttatt
    13621 acgctccaga gagtgcggaa gccgcacctc tggttgcggt actcaccagt gacggctggg
    13681 aaactcagtg gcctctacca gaagcgtcat tagcgccgct ggtgacggct tttcagacac
    13741 agtgtgaaga gactcacgcc caggcctggc agcgtttgtt tgtcggcccg tgggcactgc
    13801 cgtctccgcc gtgggggtcg gtctggctgg atcgcgaatc tgtgctgttt ggcgattcaa
    13861 cattggcact tcgtcagtgg atgcgcgaga aaggcattca gtttgaaatg aagcaaaacg
    13921 aaccggaaga tcattttggt tcactgctgt tgatggctgc gtggttggcg gagaatggtc
    13981 gccagacaga atgcgaagaa ttgctggcat ggcacctttt tccgtggtca acacgttttc
    14041 ttgatgtttt tatcgaaaaa gcagaacacc ctttctaccg tgcactgggt gaactggctc
    14101 gcttaacgct ggcgcaatgg caatcacaac tgttgattcc tgtcgcggtt aaaccgctgt
    14161 ttcgatagga tcaccgtaat attgccggat ggtgacgatt acgcgcaatc cggcaataat
    14221 aggttacagt gtcacgtttt tttatctctt aaagcacgca ctgcttttgc ggctggcctc
    14281 ttttgtcgca aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc atcatctaca
    14341 catctatccg gatctgcgca ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat
    14401 tctcgcggcc tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt
    14461 cctcaataat gactccggca gtctggtcaa tgcagcgaca aacctttccc cctggcgacg
    14521 gttgctaact ccggcgctcg gcggactggc ggcgggtttg ttgctgatgg gctggcagaa
    14581 atttacccaa caacgccctc atgcgccgac cgattacatg gaagcgttgc aaaccgatgg
    14641 acagttcgat tacgcagcaa gcctggttaa atcgcttgcc tcgctgctgg tagtaaccag
    14701 cggcagtgca attggtcgcg aaggtgcgat gattctttta gctgcccttg ccgcctcctg
    14761 ttttgcccaa cgttttacgc cacgccagga gtggaaatta tggatcgcct gtggggccgc
    14821 ggcgggaatg gctgcggcct atcgtgcccc gcttgctggc agtttattta tagccgaagt
    14881 gctgtttggc actatgatgt tggcctctct cggcccggtg attatttccg ccgtcgtggc
    14941 attgctggtt agcaatctga ttaatcatag cgacgcgtta ctctacaacg tacaactctc
    15001 agtgacggtt caggctcgtg actatgcgct gattatcagt acaggtgtgc tggcaggtct
    15061 gtgcggacca ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg tgagtctcaa
    15121 acttgcgccg ccctggcaac tggcactagg cgggttgatc gtgggtctgc tttccctgtt
    15181 cacacctgca gtgtggggca acggctatag caccgtacaa tcctttttaa ccgccccacc
    15241 actgttaatg atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag
    15301 cggttccggc gcacccggtg gggtctttac accgacgcta tttatcggtc ttgccattgg
    15361 catgttgtat ggtcgtagcc tgggattatg gttccctgat ggcgaagaaa ttacactttt
    15421 actcggattg accgggatgg cgacactgtt ggcggcgacc acgcacgcgc cgattatgtc
    15481 gacgttgatg atatgtgaaa tgaccgggga gtatcagcta ctccccggtt tattgattgc
    15541 ctgcgtaatt gcgtcggtaa tttcgcggac gttacaccgt gactctatct accgccagca
    15601 cactgcgcag catagctaag cggatgtatt gccccagttc acgctgttcg gcgcgcggca
    15661 gataaggcag ttcaccaatg agcggtgccg gaagtttttt acccagcaca tcaatgattt
    15721 ccgcataatg cgccagtcct gggttgattc ggttagccac ccagccaatg agcggcagcc
    15781 cgtcgttggc gatcgcctga gctgttagca gtgcatggtt aatgcaacct tcctgaatac
    15841 cgacaaccat caacaccggc agttgttcct gcactaccca ttcagagagt ggacgcaaat
    15901 cattcatcag actgcgccag ccgccagtcc cttctaccac gacatgatcg actttttcgg
    15961 tcaggtttgc caggccgttt gaaatgaggg tgtaattgat tgggcaactg tgcgccacgc
    16021 tactttcttc ttcgcttaac gcgataggat taactgcttc ataaggcagt tcgatggttg
    16081 aaacactctg caacaccagg gcatctttat tacgcagccc ttcgggtgtc tctttgctcc
    16141 ccttcgctac gggtttatat cccgcaaccg tttttccctg ggaggctaac gcttgtagca
    16201 atgcgcggga aaccaccgtt ttccctacag aagtgtctgt accggtaata aagaaacgct
    16261 tcagcatcac taactccacc gttatgcttc acaaatataa accaggaaaa taattaacct
    16321 tgaaagtcta agttatgctt tcctggccca aattgagata gcgcaaattt tggtagaaca
    16381 gttaaaaaat gttaaccctg caacagacga atcaacaaag aaccgttata catcgcgtct
    16441 tttaccagtg cagcccctgc catcgtgccc tggttagaaa actgagtact ctcaacgctg
    16501 atgtgctgac tatacgcagg aagggcctgc tgacggatgc tgtctgagat gaccgggaag
    16561 aggatatctg ccgctttact taacggtgag ccaatcagta ttttttgtgg gttaaataaa
    16621 ttcaccatga tggcaagaat gcgccctaca tgcgcgccca ccccggtaat gatgtctttt
    16681 gccagcagat cgccgcgcaa tgccgcctga cacaatgagt ccacggttaa cggttgtcca
    16741 tgtaacatcg agctcatgga ttgattaaga cgcagctgtg ccagctcaag aatactgtcc
    16801 acactggcga tggtttcgag gcagccgtga ttcccgcaat aacagcgttt cccatacggg
    16861 tcgacctgtg tgtggcctat ttccacgaga ctactgctgc ctgcgtgtag cagatgacca
    16921 tcggtaatga cgcccgcccc cacgttgtga tcgataacca cctgaatcac atcgcgcgcc
    16981 ccgcgtgagg caccaaacaa ggcctctgcc atcgtccatg cgctgatatc atgctgaata
    17041 taaaccggta cgccggtatg ctgctccagc gcctcgccga gcggcatctc ttttacatcc
    17101 tcgtagaacg gcatgcgatg tacaatacca ttttccgtat caataattcc cagcaaggtt
    17161 atggcaatcg aagttagacg ctcaagtttt ttctggtggc gaataaaaaa ctgatcgata
    17221 tgggaaatga tacgatccag caatggcgag tcatctttta acgccagttc ctgcgactct
    17281 tccaccacca gtttgctgct cagatcgcgc agagcaagga aaatctcccc gcgactaatg
    17341 cgcagagaaa gatagtgcca ggcttcagtt tcaaccacca gccccaccgc cggacggcca
    17401 cggttccccg cttctttgat ttccagctct tgcaccaggt gtgcttcgag catctcacgg
    17461 acaattttag tgatactggc aggagccagc tgcgccagac gggaaagatc gatacgcgag
    17521 actggaccaa gctgatcaat caggcgataa accgcgcccg cgttggtctg ctttatttga
    17581 tcaatgtgcc caggctggtt ttcagcaacc accgcatact ccctatattt tcgcgctccg
    17641 aaataatctg taggctatgg tgaagcactt caatacgtgt cgtcaaattt ttacttaggc
    17701 atgtgattaa cagcacattt ttcgggcttt ttcgctgaaa tttccctcac ctgagagcat
    17761 tcagcagatg aatacgaaag tttcgcgcag ctggactttg ttcatgatgt ttcggccaga
    17821 ccaaccacat ttcagaaacc gcatcctctt ctgcaatcgg cacccagcgc atttcgttga
    17881 gctgaacacg tttaaatgac gcaggcaaaa ttgaaacacc cagaccggcg gaaaccagac
    17941 cgatgatggt cattgcctcg cccacctcct gagtgatgac gggcgtcaaa tggtaacgtc
    18001 gcatcagccc gagaatatct tcatacagcc ctgtcccgac gtgcggatca aaaaagacga
    18061 agggttcttt cgccagttca gccagcgtta cattcgggtt atttgccagg ggatgatcgt
    18121 gcgggatcat cgccataagc ggttcatgga cgatgactgc gtgttcaagc gtctccggta
    18181 acgctgtgtt acgcagcaat cccatatcca gcgttccttc aatgagcgga gcgatttgct
    18241 cacgagtgtt catttcgcgg gtttgtaaat ggacatcggg ataatcacgg cgaaacagcg
    18301 ataacgtatc ggacaccgcc cgaataaaag gagcggacga agtaaaacca atgcgcaact
    18361 cccccgcttc cccctgatgc agtctttcag cgcgagcggc agcgtcatct accatagaca
    18421 ggatttgccg actatctgca agaaactgtt ttcctgctgc cgtcagcaat acactgcgat
    18481 tagttcgtgc cagcagtcgg gcaccaattt gttgctccag tgcctgaatc tgctgactta
    18541 gcggtggttg cgaaatattc aggcgggcag cggcgcgccc gaaatgcagc tcttccgcaa
    18601 tagcaacaaa gtaacgcaga tgacgaagtt caatattcat atttaaaaca tcttatttga
    18661 gattattaat atattagaca gaacaattcg attttcctaa cctatgtata agcctgatcc
    18721 acaggcatat ttagcaagga tttcaagtga gccgtactac aactgttgat ggcgctccgg
    18781 caagcgacac tgacaagcaa agcatttctc agccaaatca atttattaaa cgcggtacgc
    18841 cgcaatttat gcgcgtcacc ctggcgctgt tctctgccgg actggcaaca tttgcacttc
    18901 tctattgtgt gcagcctatc cttccggtgc tttcgcagga gtttggctta acgcccgcga
    18961 acagtagtat ttcactgtcc atttccacgg cgatgttggc tattggtttg ctgtttactg
    19021 gccccctatc cgatgccatt ggtcgcaaac cagtgatggt cacggcgcta ctgttggcct
    19081 ccatttgtac gttactttcg acaatgatga ccagctggca cggcattttg attatgcgcg
    19141 ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat cttagcgagg
    19201 aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc aactcaattg
    19261 gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac tggcgaattg
    19321 ctctggcggc aatcggttgt ttcgcgctgg cctcggcgtt gatgttctgg aaaatccttc
    19381 cggaatcacg ccattttcgc ccgacttcgc tgcgccctaa gacgttattt atcaactttc
    19441 gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt ttgctgatgg
    19501 ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctttcc ccctggcatg
    19561 tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca tggagctcac
    19621 ccaaagccgg aaccatgacc acccgctacg ggcgcggtcc ggtgatgttg ttttcgacgg
    19681 gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg atctttgccg
    19741 gaatgttact cttctcagca ggattcttcg ctgcccactc agttgccagc agctggattg
    19801 gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc agttactatc
    19861 tggggtcgag tattgccggg acgctgggtg gtgttttctg gcataactat ggctggaacg
    19921 gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg acgcgtttgc
    19981 atcgtcgtct gcacgcctga aaaataagcc cggactgcgg taactacccg tccggactta
    20041 ttgcttgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc agccaattcc
    20101 cgcagcgcgt ctagcgtcat caggattata agtacccaaa taaacggatt cattttgctg
    20161 tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta aaatagttaa
    20221 cccgatcaag actactatta ttggtagcta aatttccctt aagtcacaat acgttattat
    20281 caacgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc aaccactcac
    20341 ggaagtctgc cattcccagg gatatagtta tttcaacggc cccgcagtgg ggttaaatga
    20401 aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt gccgctgcta
    20461 tgggtctgtc ttctgccgcc tttgctgcag agactgcgaa cacacctgct ccgactgcga
    20521 cgaccaccaa agcagcgccg gcgaaaacta cacatcataa aaaacagcat aaagcagcac
    20581 ctgcccagaa agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag
    20641 cccctgaaca aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac
    20701 cggcaaaacc tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc
    20761 ctcgcgcctg aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg
    20821 tagtatggtg ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact
    20881 gattgccgcc cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat
    20941 cccgtcccgt ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga
    21001 gtgtgatatg cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt
    21061 tgtgtttgca gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt
    21121 ttttggtcat gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt
    21181 tgggcaactg gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct
    21241 ggcattaacg gcaggacact gtttattgac gccgcctaag ggtaaagcgg ataaggcggt
    21301 agcgctgcgt tttgtgtcaa ataaaggtct ttggcgctat gacatccacg acatagaagg
    21361 ccgcgttgat ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgttcc
    21421 tcccgcagct gcaccatggg acttcggctt gattgtgctg cgtaatcccc cttctggcat
    21481 tacaccgttg ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc
    21541 tggtcgtaaa gtgactcagg cgggatatcc tgaagatcat ctcgatacgt tatacagtca
    21601 tcaaaactgt gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac
    21661 cttgccgggt gacagcggtt cgcctctgat gttgcatacc gatgacggct ggcaattaat
    21721 tggggtgcaa agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat
    21781 ttctgttacc ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc
    21841 tgcccgacag tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa
    21901 cagcaagacc aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc
    21961 tgcggctaac gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc
    22021 gatccctttt acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag
    22081 caagccaaat attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat
    22141 ttccagcacg attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt
    22201 tcacctccag ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg
    22261 cgaccagggt ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacataa
    22321 cgctaaacaa ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta
    22381 aggcgatttt tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca
    22441 ttaaaataaa gccgccattt ccctcactga cgctcgccca tttcatggac agcgtaccgg
    22501 taatttctgt agcaatagcc agacctaata aaatccaata aatatacatt gtccttctcc
    22561 tgcaagagaa ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc
    22621 cccgcgcata gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc
    22681 tcacctacga gcaagatagt ggatgaggta cgaagatggg taaatgtaga aaataacgtc
    22741 ctgaacaaat tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat
    22801 gataattgtc ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca
    22861 aaatattttc acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa
    22921 gacattatta ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac
    22981 gctcaatggc ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat
    23041 ccgttttgcc gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttatata
    23101 taacttattt cacttaacgc attgattcaa ttaaataatt tacacaacaa aaagtgccaa
    23161 aaatcgcaaa atgactacac cagtgactac accgttcggt gcactgtatg aaacaacgtg
    23221 gaacaaatag acacaagaaa tataaaggcg ggtcatcttt ccagggggaa gcgcaccaat
    23281 tcatgagggg cgttaatgtc gatatgggga tccccaccta ccggaatttt tgccaggtta
    23341 aatgttaatc aggctggtgg gttttacata cccttgatca cggtaatgat gattcaggtg
    23401 cgtacctttt ccccagtggg gtaaaagctc caaccgtaac gatgttcgtt gtagttgcct
    23461 tgatggtctg cgtggtcatt atgaccattc agccaatgac cttaatcaga atttttcgct
    23521 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatggat
    23581 ggttaaaccg tctttatctc gatatccgta cgtattacgt acgcagtcgt tccggcttct
    23641 tccagtggta cgtaattttt gctttttccc tgtacgtctg cacgcggcga cggtaggcca
    23701 gcatttcaag aacgcggatc cgtatgttgc gcatatccac atcattaagc tggataccat
    23761 cacggcgcat cacctccgcg acaacacgcg cataattttc ggcggtcacg ctgtccggct
    23821 gcgtggcctg ttcgtcatgc tgctggctga ttccagagac gcggcggatt aatcgcagta
    23881 tttcggcttc tgtcatgctg ctgacctcat tacaccccgc taaattcttc cagtttctgg
    23941 cggtggctgt cgcttatatc aaaagcaaaa tcctcatgct ctgcctggaa tgtaccaaac
    24001 gccatcagtg ccgccacgct cgggtctatc ttgttcggtg attttttctt gttcggcttg
    24061 atattggcgt tcgcgtcact ctgcatcacg acgttactca tcgaccagga caacaccgga
    24121 tcaccacgat gcacgataac cctgcggtta acaaaaactt cgaacgattt cgccgccgga
    24181 ctgaatcgaa ggtaggtttg cgggaacggc tccacctcaa agcctgcccc ctgtaattgc
    24241 gtcctgagat gcgttgcgtt ccacgtatcg aagcccacca gcctgatatt aaatttttcc
    24301 gcgtcctgca tgatgtcatc acgtatccgg tcgtaatcaa tgcagtcccc tggcgttgtg
    24361 cgtatccatc ccgcccgtgc ccactggcga tagatagcgc gatttttatt ggctgggttc
    24421 tgtaactgga actccggcag ataatgacgg gaaaccagca tgatattttt accgaccgga
    24481 aaggcatagc acacgctgga aatgtcgctt gtggatgata aatccagccc cgcgtaacat
    24541 tcctgaccgt gtaaatcttc ctccgtgaac tttccggcgc actcagccca tgctccgtta
    24601 cccatccacg gggtagcccc ctgacaccag atattaaatc gctttgtcat catctccacc
    24661 cattgcgacg gtatgccccg cgctttctgg atggttgagg ccagtttttc acgatccacg
    24721 gaaacatcaa tattagggtt cgcctttatc cacatctccg gatcatcaac ctcgttttcg
    24781 tcgtccagtt cgtaaatcag tacaaaaatt gaatcgttga cctcttcgcc gtccaggatc
    24841 tggcagcaat aatcatagtg ctgtttacag gctgaaacga cgttgctgcc tgatgtggta
    24901 atggcaaata acagcccttc gggacgtgcc cccatcccca gttcaagcgc agaataaacg
    24961 ccgttatcgg ggtgtaagtg gtattcgtcc acaatggcaa ggctggggtt agtaccctcg
    25021 atggttgccg cttttgctgc cagcggcttt aacaggctgt tgcttttcgg gtgcatcacc
    25081 ttgtgggcct gaatattcac tctcttgcgt aacggtcggg ataaaaggca catctgacgc
    25141 gcatcatcaa aaacgatccg cgcctggtcg cggctcaccg ctgcggtgta aatatcctgc
    25201 tgcccgttct ccataatcag aaaccagtta gccagaatcg cggctgtcgt ggatttcgca
    25261 tttttgcgcg gtacttcgat aaaggcgctc gtgtatttgc gccttccggt ggccttaacc
    25321 ttaaagccga ggatgcacgc aaaggcaaac tgctgccacg gttccagctc aatgggtctt
    25381 ccacgcatcg gcccttttac gtgcgggcac accctggaaa agacaataaa ccgctcaaca
    25441 acctcacgat cgaacatgta aagggagttt tcaaggtccg aaaagtatcg tttaacggcc
    25501 tgtttcagcc gtttacaggc cggaatcttg cccgttttta cgtcttctgc gtacttattc
    25561 caggcggtca agctcgtcct cttcttctgt ttccggtgga tttttacggc ggcttatcgg
    25621 gtcaaaaccg agcaaagagg ccatttttat catcactctt tccgcgtcgg atttcgcgct
    25681 taatgcgggg tttctgctct ctccgccctg actgttaaca atgctgaacc cgcgcgccgc
    25741 aaggtctgcg acggctttcc ggtatatcga gtaattgacg caatacagtt ccagattact
    25801 ccagtcggca ggggtcaggt ctccccgttc cgcaagctgc cgcgattttt cccgccactg
    25861 cttcacggcg atatcatcca ggtaggcggg ggctttcggt ggtcttgcca tgcttatttt
    25921 ttcgccagat tatttttcaa aaaattaccg tgcataaaaa tttggggagg cgttcggttc
    25981 tgggcggggt cgggttcgtc ctgaaaactc cccccacccc gtccggctgc ctcatcagcg
    26041 attgcggaaa cattccataa cctcgcggtc acggtcgttt aatcgcgtcg ctgtgatgcg
    26101 ttctgcgcgt cctgatgctt tatctttatg ccctgcttcc tgtgccttcc atgcgtcacg
    26161 ctgccttata agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgccataca
    26221 tccagttatt gcggttagcg ccccgttctt cctcctcacg gaatccaccc gcagcacgct
    26281 tgttttttgt ggacggatca agccatttcg ttttctggtt atgacacgcc tggcacaatg
    26341 gctgatgatt ccactcaggc cagaagagaa catcattacc gccatcgatt gggataatgt
    26401 gatccaccac cacggcgggc gtgtatattc ctttttccag gcaatgcgca cataacgggt
    26461 ttttactcag atacatggcg cggtatttct cccactgcct cgtataccct cgctccctgc
    26521 tgctgcctct ccggctgtcc tgctgctggc gtgcgctgcg tctgtgctcc tcacacttgc
    26581 cggacttcac gcgcctgttg cagcctggtt ctgtacatcg tcttaatggt tgccacggca
    26641 tcagtacacc cccacatcac ggtaagccgt ccagagtgcg ccaatcgtca tgggtacgtg
    26701 tgtttttgcg ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat
    26761 gcagccaatc tttatggctg gcgtgaactc cagcccgtca tcaaagcgcc tgcctatgtg
    26821 tatctggcac gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc
    26881 gccatcgatg cggcaatgga gtttcacttc ttccagggtg ataagttctg tcatttttcc
    26941 gcgccctcac tacaaagaat ttcaaggcgt gtcctggcgg catctggcag cggttgcccg
    27001 atgatattca gtacacgccc cgccagcggc cccgtattga cctttatccg actggtggcg
    27061 ttgatgtcct tccggtagcg tatccatatc cttacagttc cagtcgccag ctctgcacct
    27121 gatgaaatgg cctccttgct gctgatcatg ttcacgcttg cccagagtgt gtgaccgtcc
    27181 acccacgttt cgagtatttc gcccgtcatg gctctggtct gtttcagggt ctgaatcgtt
    27241 accctgtcac gcagtcgccc tatgttcatt ccggtttttc tccctcgctg atttttactt
    27301 cctgtttcca tgcctggctg aactcatcac caccatcacg cggtgacagt ccttcacgtt
    27361 cgcgggcttc gttcggacac ataacgccgg atttgatgcc gcgctcatag gtggcaaagc
    27421 gttcgccagg tgtggcccgt aacaggtccg cgctgtcaaa ctccacctga taccggatac
    27481 caggtacagg cgaggccacc agcagggcgt tttttatctg ctgctcaaag ttcgccagcc
    27541 acgggcgcat cgtcatggtg agaaatgcgc ggcttgcctc gctgaaattg ctgtaggtgc
    27601 tgttgctgta ttcctgcaaa aaaatcggcg agacgttgaa cattcgggcg atgtcttcaa
    27661 tggtgaagcg acgggaggcc agccattcag catcctgatt actcatgccc agctgctgat
    27721 agctcatacc cccttcaagg atgggcgttt ttccggcgtt tctggcccct ttgtagcgtt
    27781 ccagtgcggc taatgcctgt ttgcccttca cgccgtccag ccattcgcct gacgtgataa
    27841 cccctgccgc catcatgcca tcacgcatca cgctcgcgcc gtggcgttgt tgggccagcc
    27901 caagccccag cgattcgcgg caaatggtta caggtgagcg ccccataaaa ccgtcatccg
    27961 tggagtaacg aaggtggaga acctcccacg gtaaatagtt gcgggtgttt ccggtgtaag
    28021 cgtcagtgat gcagtagcgc cagttgtgtt ctcctgtctg ctccacgttc accgactgcg
    28081 gcgggtaagg atgtaaagcc gccggaaaac catcacgccc ccactgaatc accgcgtaag
    28141 cattaccgtt taacaggcag tggcggatca tcattcgctt aaactggtag ggagtctgcc
    28201 acgcgttcgg gcgctcgttg aggatgtgat cgaccggatg agaatcaagc cactcgcggg
    28261 cctccttccc cttctcattg cgtaccaggt acaggtagca tggcatagta gccaccgcct
    28321 cagagatgac cgtgacggcg ttcatgacgg cggggagtga ttccgctgtc cccgatgata
    28381 cgtactcacc cgcccccgtg ttcgatgtgc cagccagcgc cataaattca tcaagcgtca
    28441 tgctgcgctg ctcttttttt cttctgaaag gccacatatc acacccccgc taaatccgcc
    28501 caccagcggc gattatccgc acgcggcatt ttttcggggt gctgctcata cagggaacgg
    28561 cgggccagct ccacgccgga atcggggtaa gccggtacgg atgtaacggt aatttcgtac
    28621 agttccgcca ccagcacggt gcgcacgcat ggatctgttg tggtatccca tacatcctta
    28681 cgggaacgaa agccaaagct catgccggat atatcaccac gtttaaccag ttcgataacg
    28741 tcacgccctg tgctggtatc cggtggggta agttcaaagc gtaaccctgt gtcgtcctct
    28801 tccagtttca gcgtgccaga acgggtgcgc cccagtaaca tgctgtggtc atgctcatac
    28861 aggccgcgaa cgtcattacc cgccgcaagc cactcagtaa acgccccccg ctggaatttt
    28921 tcgtaaaact ccccccataa cagttccgaa aggttatccc agcgaacaac gtaacccgtc
    28981 agcgtgttgc tggcgctggt ggtgatttcc gatgaccgga tttccatact cttcataatt
    29041 tttttcaccc ataaaacact aaaggggctt tttagcccct tctgtatgct gttaatcgtc
    29101 gtcctgtggc agttccagaa tcttgatcgc gttcgaatcc accacgccac cgcctaaata
    29161 tttctgcgtg aaaattttga tgaatcctgg ttctgtcagg ttgtccggtc tggtgcgaac
    29221 acccgtttcg tgatcaacaa tgtagtaacc gcgtttgaag tcacccaggg caataacgtt
    29281 atccggcata aactccagat attcgaccgg aaggcccagc aacgtatcag gatcaccagc
    29341 ctgtaaacgg tcgcgccaga tgtaatcacc gttcgcgttc ttcaccttct gaagttttgc
    29401 cgccgtcgtg gagttaacca cccagaccgc gtttttgcgg tattttttac gtaatgcaaa
    29461 tttcaggtcg atcagcgggt ccgcagatgt ccacgcaaga gactcagaag gcttaattac
    29521 ctgcaacgta ccaaaatcac gttctttgtc gttcttctct gcacggggta cggataaaaa
    29581 gccttttgct tttttgtcac cgtcgcccac aaccagatcg ctttcttcgg tttccgtgaa
    29641 ggtgtcgcca atctcacccg tcagccatga aaggatgtcc acatcggaaa aatccacgat
    29701 ttcctgtgtg gtgcgcgggt acgcatagac cggatacagc ttaatgctca cctcgttaat
    29761 ctgcggggtg ctggtctgtt cgcgtgcctt accctcttca ccgtggttaa cggtcgcacc
    29821 gccagcggaa acaagctgct taaactcgtt gctgctgatt ttcttcacgg tacagatgcg
    29881 gcgcatggtg gattcatccg ccagcattcg catgatttcg gtgttcagct cggggataac
    29941 ggtataacca ccatcagcgg gaacgcctgt acttaatgcg cgggtttcac ccgtcagaat
    30001 gtagtttcgt agttctgcgg ggtcagtggt ctggctgctt ttacctggct tgctgcgctc
    30061 ttcgtctgca atggcttcaa ggcgggaaat gtctttatcg agggattcag ctttagcgcg
    30121 taattcgtca aattttgcgc cctctgcatc gttaaggctg cggttttctt tttccgcgtt
    30181 ctccagcatg tcgcgcatct gatttttaat ggcggttttc tgctggcgta attcgattat
    30241 tctcggcata aaaaaagtcc tggggttaag taaggaactc caggacgcgg caaaaactca
    30301 accgtttttc ataaggaaat cagcaatcgc accgatcgtt tttccgcctg gtaatgaata
    30361 ttggtgagca cattaacagg cgggaaagtg gccccagcgt cctggcacca ctggcgagaa
    30421 taatcatgat tcagggcggg taaaatatgc cgatccggtc aatgaacaac gtggaacgac
    30481 cacgaacaaa taatttacaa aaaatgacaa aaagccggat tgctccggct gtatgggtgc
    30541 tcagtccctg atccctttca gaaattctat caatgcatct atctgttcag gatttactgc
    30601 cagcatttca ccggatagcg cacatctgac aaatccatgc cgatcctttt cgattagcgc
    30661 gcccgtttcc aggaatgcgc ggtaatcatt gatgttcatc gtctccatgt tgtcagcatg
    30721 gtatttatca cgctgtttta atatctcatc aaatttcatc ggcattgttt tttcctctgt
    30781 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc ggtaatgcgc
    30841 cataccgttt caactggcgc aaaaaaagcc ggatttctcc ggctgtgtga ttagctgtcc
    30901 tggtaattgc gccgtatttc atcaccagca ccatctatac ccatttcggc acaagtgcga
    30961 ttaactgcat ttctcaggtc tccgaaatta tcaggcggct ccggtggcct ctgtgccttc
    31021 ctggaacatt ccagccgtcg catcgtgatg tgatgccgtt ccttgtctgt ctccaccagc
    31081 atcatgactt caccccatcg cgccgccgcc ctccggtaaa atcctttcgc ttcgagttct
    31141 tccgctatgc ggtcatgtac catcgtcacc ccctcagaac ggaatatcat caccgtaagg
    31201 gtcatcgcct cccgctggtg gctgattacc ctgtgtgcct gtggttttgc gtctgctgcc
    31261 gcctggtcgc accgttttcg cactgattac gctgtcggca atgacctgat aaccctgacg
    31321 cgtctccccg ttctgtccgg tccactggct tatctgcatc gtgccggata cgctgattac
    31381 gttgcctttc tcatgtcctg ccagttggtc ggcctgtttg ccaaatgcca ggaccgacag
    31441 ccataacgta gcctgcccgt cctgcgcctg actgcatggc agtgataccg ccatacgcgc
    31501 cagcgtcatc ggtgtgccct tgctggtctg ttttacctgc gggtcgtcca ccagccgccc
    31561 gtaagctgca atttgtgctg tcatattgcc acctcagtga agcgatccgg cgttgttctt
    31621 tttcatctct tccatcattt tttcagtgag cattacgcac tgaaaaccca tgtttttgat
    31681 gtctacgggt ttcatgcctt tgtcagtgga tatcacaggc agaatatcct gatacacatc
    31741 gcggtcaggg taactaagca cacaaagcgc ccatgatgcc acgggtgagg atccttcgtc
    31801 tgtttcaacc caccagcctg gtgtagctgc gcttatttgc agaatcgtga tccgtccgtt
    31861 gtgcttgtaa aatttttcac caaattttgg ttcgctcatg attccacctc tccggtttta
    31921 acgttgatgg ttgttacctg ttccgcttcg gcaatctccc gttctgtcag cgtggcaaag
    31981 tttgcagctg ccgttgtcat gaatgcgctt attagttcgg gattctcctt cgcgtatcct
    32041 tcccgtgtgt ggcggtctat aactttgatt gccaccttta gggaaagctc tgtcatgtct
    32101 aacgctttat atttttgctg tgttctgtct ctgcgcattt tggtcatttg tcgcccctga
    32161 ttcatgtttt cggtcggcat gtttgttaag tgattttgat gtatgcacat ttatttttac
    32221 cccctcgttt aaaaagtttt aggttgtggt gcacctcctc tacctctcta cctaatcatc
    32281 tttcaggtca gtaatggcgc ggctttcaga tgggtagagg gctttcgtgg ccctctaccc
    32341 atcatctacc cgcccctcac aaacaacctt aatcatggtg gagggggtag agggctttta
    32401 tttgccttct accagcccct c
//
LOCUS       DAZFWP020000041        32341 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFWP020000041 DAZFWP020000000
VERSION     DAZFWP020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.041"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            218..2137
                     /gene="ygfT"
                     /locus_tag="ACHK0Z_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             218..2137
                     /gene="ygfT"
                     /locus_tag="ACHK0Z_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU4501817.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            2137..2625
                     /gene="ygfS"
                     /locus_tag="ACHK0Z_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             2137..2625
                     /gene="ygfS"
                     /locus_tag="ACHK0Z_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4501818.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(2661..4028)
                     /gene="ghxQ"
                     /locus_tag="ACHK0Z_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /gene_synonym="ygfQ"
     CDS             complement(2661..4028)
                     /gene="ghxQ"
                     /locus_tag="ACHK0Z_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU4501819.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(4064..5380)
                     /gene="guaD"
                     /locus_tag="ACHK0Z_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             complement(4064..5380)
                     /gene="guaD"
                     /locus_tag="ACHK0Z_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU4501820.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(5398..6798)
                     /gene="xanQ"
                     /locus_tag="ACHK0Z_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             complement(5398..6798)
                     /gene="xanQ"
                     /locus_tag="ACHK0Z_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU4501821.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(6963..9833)
                     /gene="xdhD"
                     /locus_tag="ACHK0Z_RS08940"
                     /old_locus_tag="pgaptmp_001788"
     CDS             complement(6963..9833)
                     /gene="xdhD"
                     /locus_tag="ACHK0Z_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU4501822.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(9830..10609)
                     /gene="ygfM"
                     /locus_tag="ACHK0Z_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             complement(9830..10609)
                     /gene="ygfM"
                     /locus_tag="ACHK0Z_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU4501823.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(10660..11988)
                     /gene="ssnA"
                     /locus_tag="ACHK0Z_RS08950"
                     /old_locus_tag="pgaptmp_001790"
     CDS             complement(10660..11988)
                     /gene="ssnA"
                     /locus_tag="ACHK0Z_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU4501824.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLHEAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(11991..15089)
                     /gene="ygfK"
                     /locus_tag="ACHK0Z_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             complement(11991..15089)
                     /gene="ygfK"
                     /locus_tag="ACHK0Z_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU4501825.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(15411..15989)
                     /gene="mocA"
                     /locus_tag="ACHK0Z_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             complement(15411..15989)
                     /gene="mocA"
                     /locus_tag="ACHK0Z_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU4501826.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            16092..16862
                     /gene="yqeC"
                     /locus_tag="ACHK0Z_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             16092..16862
                     /gene="yqeC"
                     /locus_tag="ACHK0Z_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU4501827.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            16910..18535
                     /gene="yqeB"
                     /locus_tag="ACHK0Z_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             16910..18535
                     /gene="yqeB"
                     /locus_tag="ACHK0Z_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU4501828.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(18576..19508)
                     /gene="arcC"
                     /locus_tag="ACHK0Z_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             complement(18576..19508)
                     /gene="arcC"
                     /locus_tag="ACHK0Z_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4501829.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(19556..20941)
                     /gene="hyuA"
                     /locus_tag="ACHK0Z_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             complement(19556..20941)
                     /gene="hyuA"
                     /locus_tag="ACHK0Z_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU4501830.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(20994..22205)
                     /gene="ygeY"
                     /locus_tag="ACHK0Z_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             complement(20994..22205)
                     /gene="ygeY"
                     /locus_tag="ACHK0Z_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU4501831.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(22263..23459)
                     /gene="dpaL"
                     /locus_tag="ACHK0Z_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             complement(22263..23459)
                     /gene="dpaL"
                     /locus_tag="ACHK0Z_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU4501832.1"
                     /translation="MSVFSLKIDIADNKFFNGETSSLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(23517..24704)
                     /gene="ygeW"
                     /locus_tag="ACHK0Z_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             complement(23517..24704)
                     /gene="ygeW"
                     /locus_tag="ACHK0Z_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU4501833.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            25183..26955
                     /gene="ygeV"
                     /locus_tag="ACHK0Z_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             25183..26955
                     /gene="ygeV"
                     /locus_tag="ACHK0Z_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU4501834.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQVEIVDENLCRVAGTG
                     AYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTPVI
                     LQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISKIF
                     ATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGH
                     MQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRV
                     MRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQL
                     LESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREI
                     LPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIE
                     LLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGEVI
                     DSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSKKQ
                     VADELGIGIATLYRKIKKYELLNT"
     gene            complement(26995..27474)
                     /gene="xdhC"
                     /locus_tag="ACHK0Z_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             complement(26995..27474)
                     /gene="xdhC"
                     /locus_tag="ACHK0Z_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU4501835.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(27471..28349)
                     /gene="xdhB"
                     /locus_tag="ACHK0Z_RS09010"
                     /old_locus_tag="pgaptmp_001802"
     CDS             complement(27471..28349)
                     /gene="xdhB"
                     /locus_tag="ACHK0Z_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU4501836.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(28360..30657)
                     /gene="xdhA"
                     /locus_tag="ACHK0Z_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             complement(28360..30657)
                     /gene="xdhA"
                     /locus_tag="ACHK0Z_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU4501837.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINKPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            31072..31827
                     /gene="actS"
                     /locus_tag="ACHK0Z_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             31072..31827
                     /gene="actS"
                     /locus_tag="ACHK0Z_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU4501838.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            31906..31979
                     /locus_tag="ACHK0Z_RS09025"
                     /old_locus_tag="pgaptmp_001805"
     tRNA            31906..31979
                     /locus_tag="ACHK0Z_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31938..31940,aa:Gly,seq:ccc)
BASE COUNT         7861 a         8011 c         8366 g         8103 t
ORIGIN      
        1 attctggcat gtcgcactct cgcatttaat cgtttttatc tggatagcgc tcttttggtc
       61 ggcaatgttg aaataatgaa agcggcatag attgaggggc aataatttga actcagtcgc
      121 aaaattataa ttaaaacggt catgctacct aatatatgcc cgtttattgc atcgcgaaat
      181 ttgagaagac ataactcatt aaatgaaagg gatgcaaatg aataagttta tcgctgctga
      241 agctgcggaa tgtataggct gtcatgcttg tgaaattgcc tgtgcggtgg cacacaatca
      301 agaaaactgg ccgctgagtc acagtgactt tcgaccgcgt atccacgttg tagggaaagg
      361 ccaggctgcg aatccggtgg cctgccatca ctgcaacaat gccccttgcg ttacggcttg
      421 tccggttaat gctctgactt tccagtccga tagcgtacaa ctggacgagc aaaaatgtat
      481 tggctgtaaa agatgtgcaa tcgcttgccc ctttggcgtc gttgagatgg tcgatacgat
      541 tgcgcagaaa tgcgaccttt gtaaccagcg cagttccggc acgcaagcct gtattgaagt
      601 ctgcccaaca caggcgttac gactgatgga cgataaaggg ttacagtgga taaaggtggc
      661 tcgccagcgc aaaacggcag caggaaaagc gtcatcagat gctcagccat ctcgcagtgc
      721 agcgttgctc cccgttaact cgcgtaaagg cgcagataaa atttcagcga gtgaacggaa
      781 aacccacttt ggcgaaatat attgcgggct ggatccactg caagcgactt atgagagtga
      841 ccgctgtgtt tattgtgccg aaaaagctaa ctgcaactgg cattgcccgc tgcataacgc
      901 tattccggat tacatccgtc tggtacagga aggaaagatt attgaagcgg cagaactttg
      961 ccaccagacc agttccttac cagaaatttg cggcagggta tgtccacagg accgtctttg
     1021 tgaaggcgca tgtactttga aagatcactc gggcgcagtc tctatcggta atctggaacg
     1081 ctacatcacc gataccgcgc tggcgatggg ctggcgtccc gatgtcagca aagttgttcc
     1141 ccgtagcgaa aaagtggcgg tgattggcgc tgggccagca gggttagggt gtgctgatat
     1201 tctggcgcgc gcgggagttc aggttgatgt ctttgatcgc catccagaaa ttggcggtat
     1261 gctgactttt ggcattcctc ctttcaaact cgataaaacg gtattaagcc agcggcgaga
     1321 gatattcacc gcaatgggaa tcgactttca tcttaactgt gaaattggcc gcgatattac
     1381 ctttagcgat ttaacttctg aatatgatgc agttttcatc ggcgtgggga cttacgggat
     1441 gatgcgagca gatctgccgc atgaagatgc gcccggtgtc attcaggctc taccgttcct
     1501 gactgcccat acccgccagc tcatgggatt gccggagtct gaagagtatc cgctgacgga
     1561 cgtggaaggt aagcgagtcg tggtattggg cggtggcgat acgacaatgg attgtttgcg
     1621 gacttccatc cgcctcaatg ccgccagcgt gacctgcgcg tatcgtcgtg atgaagtcag
     1681 tatgccgggc tcgcgcaaag aggtggttaa tgcgcgcgag gaaggtgtcg agtttcagtt
     1741 caatgttcaa ccgcaatata tcgcttgtga cgaagatggg cgtttaactg cggtgggcct
     1801 gattcgtacc gccatgggtg agccggggcc ggatggtcgc cgtcgtcctc gtccggtagc
     1861 gggttcagag tttgaattgc ccgccgatgt tctgattatg gcctttggtt tccaggcgca
     1921 tgccatgccg tggttgcagg gcagcggaat taaactcgat aaatggggcc tgattcaaac
     1981 cggtgacgtc gggtatttac ctacccagac gcatctgaaa aaagtctttg ctggtggtga
     2041 tgcagttcat ggcgcggatc tggttgtcac tgcaatggcc gcaggaaggc aggcggcgcg
     2101 cgatatgtta actctgtttg atacgaaggc atcgtgatga aatcgttaat tatcgttaat
     2161 ccggctgact gtattggctg ccgtacttgt gaagtagcct gtgtggtcgc tcacccttca
     2221 gaacaggagt tgaatgccga tgtcttttta ccccggctga aggtacagcg actggacagc
     2281 atcagtgcgc cggtgatgtg ccatcagtgt gaaaacgccc cttgtgttgg cgcttgcccc
     2341 gtgggggcgc tgacgatggg cgagcaggtg gtgcagacca attctgcccg ttgtattggc
     2401 tgtcagagtt gcgtcagtgc gtgcccgttt ggaatgatca ccattcagtc gttgccgggg
     2461 gatacccgac aacaaattgt gaaatgcgat ctctgtgaac agcgggaaga ggggccagcc
     2521 tgtgttgaat cttgcccgac acaggcgctg cagttgctta ctgaaagaga actcaggcga
     2581 gtccgccagc agcgtattgt tgccagcggt gagaatcctc tgtgaaagaa atctccgcgc
     2641 tgaaaggcgc ggagaatcta ttagattgcc caaccacccg cgtaaaatgc gaccagtgcg
     2701 gcagtaatga tcaccgtacc aatattcaac ttttgccatt cgcgagcaaa gacacggcct
     2761 acgaccagtg tcacaaagcc cagcataata ccggtaacga tattacaagt cagaacgatg
     2821 aacacggcac acaccaggcc agccattgcg tcaataaaat cgttgaaatc cagcttcgag
     2881 acgttactta acatcagcaa acccacgtac atcagagcgg gtgcagtggc gtaaccaggg
     2941 atcaaaaatg acagcggtga taagaacaga atcagcagga ataacgcccc cactacggtt
     3001 gcggtcaacc ctgttttacc cccggcggcg gttcctgccg ctgattcgat ataaaccgct
     3061 gcgggcgctg cgcccaccag accggagaat attgaactta ctgagtcact ggtcagggct
     3121 ttgccgccat tgatgatctg gttgtcttta tccaacaaat tcgcctgacc ggcgacggca
     3181 cggatggtgc cagtggcgtc gaacactgcg gtcatcacca atgccagtac actcggaagt
     3241 acggttggct ggagtgcgcc cataatatcg aggctgaaaa tcagagactt accatcttcg
     3301 ccagtcagac ttggcatcgc caccagaccg tggtatttca ccgctgggtc aaagattaag
     3361 ccgataatcg aaattgcgat aatcaccaac aagatcccgc cgggtacgcg gcacttctcc
     3421 agaccgaaga tgaccgccag ccccagcaag ctcatcatca ccgggaagga ggtaaacgca
     3481 ccgagcgcca ctggcaagcc ttcaatcggg tttttgataa ccatacccac accgttagca
     3541 gcaatcagca gcagaaacag gccgataccg atacctgtac cgtgagcgat acccatcggt
     3601 aaattacgta agatccaggt acgcacaccg gttacggaaa tagcggtgaa gatgacgccc
     3661 atcagaaaga ccgcgcccag tgcgacagga acgctaattt gttgcccgag taccagactg
     3721 aatgcggtaa atgccgtcaa ggaaatcgca caaccaatcg ccattggcaa attggcccac
     3781 aaccccatca gcaacgagcc gaagcccgcg accagacagg tggcaacaaa cacagctgcg
     3841 ggaggaaaac ctgctttgcc cagcattccc ggaacgacga taacggaata aaccatggcc
     3901 agaaaggtcg ttaagccagc cagtacttcc tgacgaacgg tactgccacg agcggtaatt
     3961 ttaaaataat tatccagcgc gccctgtggc tttggtgcgt ccggtgtttg taggatgtct
     4021 ccagacataa tgatgtcctc acgaggatgt taaagtttta ttattagttg cgttcgtaca
     4081 ccagacgacc atcaacgtag gtgcggtaga tcgaacggtc atcgcccaac gtcatcatca
     4141 cgaacaattt gtcgactaaa gaaacagagt tgtcatagcg cagctgctgt agcggagtgg
     4201 cggtgggttc catcaccacg aaatcagcct ctttgccagg taaaaagttg ccaatcaaat
     4261 cgtcaaggcc cagagatttc gctccgccga gcgtggccag gtaaaacgct tcatatgccg
     4321 agaggcgata gccttgtaat tgcaatactt tgtaggcttc gttcagcgtt tgcagcatgt
     4381 tgaaagtggt tccggcaccg atatccgttc ccatgcccac tttaactttc ttctgccatg
     4441 cttttttcaa gttgaataag ccgctgccga ggtaaaggtt ggaggtcgga cagaaagcaa
     4501 tgctggattt ggtttcgctg agacgatccc actctttttc ttcgagatgg acgcagtgag
     4561 caaagacaca gtttttaccg gtcaggccgt actgatggta aacatccaga taaccatcat
     4621 ggtcaggata aagcgatttc acccaggcaa tttcatcttt gttttcacag agatgggtat
     4681 gtacccacgt atccggatat tcttctttca ggcgttgcgc catcgccatc tgttcaggag
     4741 atgaggtcgg ggcgaagcgt ggcgtaatcg catatagcag acgaccattt ttgtgccagc
     4801 gttcaatcag ttctttgctt tggtgatagc tgctttcggc agtgtcgagc agataatccg
     4861 gtgcgttgcg gtccatcatc accttaccgg caatcatacg catattgata tgactggcgg
     4921 cttcaaacag cgcatcaaca gattgcggat gaacagtgcc aaacaccagc gccgtggtgg
     4981 ttccgttacg taaaagctgc ttgatgaaga atgccgacat ttcgcgggcg tactctaaat
     5041 cctcataacg acgttcagta gggaaggtgt gtttattcaa ccactccagc aattgttcac
     5101 cataggcccc caccatttca ctttgcggat aatggatatg tgtatcgaca aagcccggta
     5161 ctatcagttt gccgcgatag tcgcgcacgc gaatagtgtc aggaatttga tgctttccgt
     5221 cttcccattc gccaaaccat tccacttttc cctgtttaat gagtaataaa ccatcctcaa
     5281 taaaccgcag cgcagaggca atctcttccg ggttatcgac cgtacgggtg acatcaataa
     5341 aactgcctcg taccgctttc aacgtgtgtt ctcctgacat cattgactcc tttactgtta
     5401 atccatctct tccgctgagg taataccagg cagaacgttt tcctgtcggt agccaccagg
     5461 gaggataata tttaataaaa tcgcagttaa cccgccagca caaatagggt tttcaactag
     5521 tacataaata gaggctggca atattttaaa aatttcggga tcgtaggaga cgccaagtcc
     5581 taaacctaaa gaagtggcga caataagtgt ttctcggcgc tttaaaccgt tggtgatgat
     5641 tatgcgaatc ccagcgatgg caatcatgga aaacatcaac gtcattgcgc ctcccagaac
     5701 tgccgaggga atggtcgtga agaagccgcc aatcatcgga aacaaaccga ggataaccag
     5761 cattaccgcg atggttcgcc cgacataacg tgaagcgacg ccagtcatct gaataacccc
     5821 attattttgc gcaaacgtgg ttaagggtaa tgacccgaca gcggaggcga taacagaaac
     5881 cagaccgtct gccagcacgc cgcctttcag tcgggactga tactcttctc cctgaatggg
     5941 tcggcgggaa accattgccg tggcggtgat atcgccgaca gcttccagca cgctaagcag
     6001 ataaatcgtg ccaaccacca ggaactgatg gaagctaaaa ctaaagccgt atttgaacgg
     6061 atgcgggatg gtgattaacg gcaaattgcg catactgctg aaatccacca tgcccaggca
     6121 taacgatgcg atatagccga cacatagccc aatggcgatc cctcccatgc gtagcaacgg
     6181 actgcgacag cagttaaagc cgatcaccac aattaaaacc aataaaccaa cgccgagatg
     6241 ttcgtaattg ccgaatgtac cgctgctttt ggctgcaaat cctccaccaa aatcgataat
     6301 gccgacttta atcaggctta agccgatcat cagtaccaca ataccgctga cggtaggcgt
     6361 aataacccga cgtaaatagg gcaagataaa tgaagatccg acaaccagaa atgcgccaac
     6421 gaaggagacg ccgagaagcg acgacatgat taactcttcg tgaaaaccgt cgcttttcat
     6481 gctgctgccc agcgcaatca tgaccgtaac aaatgaaaaa ttgactgact gaattgagag
     6541 taggccagaa ccgacgatgc cgtagcggtt tacttgtaac caggtaccaa taccagaggc
     6601 gatcatcgcc atagaaacaa gataggcagt tgtttcagcg gaaagctgta aggccgcccc
     6661 cacgattaac gcgggggtta ccatcggaac gaaaattgcc aacagatggg taatggcacc
     6721 aacgagagcc tgatgaaagg gagggcgatc ctccagttca aatataaggt cagaacctgc
     6781 attgtttata tcagacatcc cttcactcct tttaaagtaa atttgatcat ctgaattagt
     6841 aaaataaatt cccacgaatt tggggcgcag gacgccttga gaacggctta tctcattttc
     6901 gcagtcacta tttagtcatc gtccaaacag agaaacaggc cgccgaagcg gccatttatt
     6961 atttatattt tttccagcgc agtgagtatt ttctccggtg tgaaatgcca ttcgcgtaac
     7021 cagatgccgc atgcatcgtg aattgcggta gcaatcgccg gagctgcgcc atttacaccg
     7081 atttccgaga tcgatttcgc cccgaacggg ccgactttat cgtcgctcgg caccagcaca
     7141 gcgcggaaat cacgcggaat gtcaccaatt ttcggtgcgc cgtaactgcg taaatcacgc
     7201 gttaacgggt gaccttcggc gtcataaatg atctcttcgc tcatactgtg gccgatagct
     7261 cgcagggtag caccgtagat ttgtcccaat gctaattccg ggttgaccgg tgtaccgcag
     7321 tccagcaagg cgtagaattt atccaggcgg atttcacccg tacgcgtgtt gacggcaact
     7381 tcagcgaagt ttgcgccata cgggaaggcg aaatcaggcg tgatataact gccagttccc
     7441 accagtgaac caaagccggt gccggtttcg cctttatgag caatatcccc gaagctgact
     7501 tcgcctttct tgccgcgcac gacgcccggc gttgccagtt gaacatctgc cactgcctca
     7561 cccaacattt gcgcgccgtg gaacagaatt ttctcccgca gattttccgc tgccaaacgc
     7621 gccgcgttac ccgagaagca agtaccggac gaggcatatg cgcctttatc aaacaacgca
     7681 tgatcggtat caccggagat aacatgcacg tcctgcggtg ggcagtgcag cacttctgct
     7741 gccagtttcg tcactacagt atccagacca gtaccaatat ccgcaccgcc agaatgaacg
     7801 ataaaggtgc cgtccgattc cagtttgatc atgcagttag cctgatcgat atccgggata
     7861 cccgatttct gcatgataat ggcgacgccg cgaccgatgt gccagtcact attttgcggt
     7921 ttcggtgaag accattggat catctcgcga ccctgacgca ggatctcttc cagtgcgcag
     7981 ctggcggcgg aaggaacgga ggtcggcgct ttaccttcac cgattgcacc gagaattttc
     8041 agctcttgcc cttcgtgtac ccggttacgt tcgataattt ccagttggtc gatctgtaac
     8101 tgttcagcca gttccgctaa tgccatggtg atagcgaagt tacctttcgg tgcgccataa
     8161 ccctgataag caccatttgg gcaaatgttg ctgtagtagg tggtgacctg gaaatcgacg
     8221 ttatcgcacg gatataacgg cagcgacagc gccggtccgt tacacggtac ggtgagtgag
     8281 tggttgccgt aagggccagt gttggcgcgg aaatccatct tcactgccgt caggcgacca
     8341 tcttttttcg ctcccagttt gacggtgact ttcgcgacgt gacgagaggt gttagcaata
     8401 aactcttctt cacgggtgta gcggaacagt accggacgcc cggtcacgca ggttgcccat
     8461 gcgcacactt cttccagcag gatgtcctgt ttggagccaa aaccgccgcc aactcgctct
     8521 ttaatgacat gaactttatg ctgtttcatg cctacgaggc gcgcgacctg gcggcgtaag
     8581 tgccatggta cctgggtgga ggcgtggata accagacgat cgccgtccat ccgggtaaag
     8641 cagatatgtg tttcagtcgg gcactgctgt gcctgcgttg agttataggt tcgctcaatg
     8701 atcacatcgg catcggcaaa gcctttgtcc atatcgccaa tatgaccatg aatactggcg
     8761 gcgatatttt tgcgagggcg agaaccgatc gggaagttga tgatcatatg ctcgccgcgc
     8821 tgggctgcat ggctgttatc gtcttccaga gtatctggcg caccagcaac atacaccacc
     8881 ggttcatcgt gcacgacagg cgcatcttcc gccattgctt cgtcgatcga cattaccggc
     8941 ttaagcactt catattcaac gtcgatgagc ttcaatgctt cgagcgcaat ttcttcactt
     9001 tctgcgacga ccgcagcaac acgatcgccg acgtgacgca ttttcttgcc gaacatacgg
     9061 cggtcaagcg gtgacggttc cggtgcgctc tgaccacccg gggtatagta gatatccggg
     9121 caattcaggt gagtaataac gtgaacgacg cccggtaagg cttcagcttt gctgacatcc
     9181 agatgagtaa tcagtgcgtg agcgtgtggg ctacgtaaca ttttaatgac gcaggcgtca
     9241 gccgttacgc ggtcttcaac atagcagggt ttcgcctgca ccattttcgc ggcatcagtt
     9301 ttaggataat gcttgccaat gacttcaagg tcgtcacgga aagtcggagc gatatcaatg
     9361 gtggcctgcg gattattttt acgtgcaacc gccagttcaa tgacctgata gtattgttgc
     9421 cagcctgcat cgcggctgaa caaaccagaa agcgcgtcgt cgatctcttc gcgggtaggt
     9481 gcggcgatgc gatcgagaag atcggtgata atcagagctg cagctggatc gttataacca
     9541 gactgcacca cgccaacatc aaccattgcc tgttgaacca gacttaactc gttccatttg
     9601 cccaaagatt ctgcggtacg aatatctgcc ttctctaact gtgcggcaat aagcaaggac
     9661 gcgttaacga tattaccgtt aaagattatt gcgtcagaac cggcaaaccc gaagccatca
     9721 tcactgttgc gtacagagtg cattcccatg ttaaacaaca gcttttgcac gttttcgcct
     9781 ggattaacgg ttagctcctg aggcgcgcca tttaaagtaa agtggatgat catacggctt
     9841 cctccccagc ctgttggcag tcggcataca ggtcggctac cagaactccc gtgatatagc
     9901 gtttataggc cacgctgccc cgcaaatctt cctgcgggaa aatggcgttg gcgacagcct
     9961 gttcaagtgc attgccttcc agattttgtt tttcgacatc atgcagacgc agtgctttac
    10021 tggcgacgcc atccagcgca attcgcatac cgtcgtggtc tgtcattgca acggctgccg
    10081 tcacgacggt taaacctgcc tgagaacggc taattttgcg ggtcgcacag gtgcgatacg
    10141 gatctttaat gataatttcg gttagcaggc gatcgcatgg gcaggccagg tagtcctcga
    10201 ttgacagcgt ttcgccgttg ccaaaaacca gttcagcatc cagtgccaac aggacgggaa
    10261 gcagcaccga ctcttcctgg cgggcggcga tttcaccacc aatggtcgac tgattacgaa
    10321 catggcgtga gtaaacaaaa ccgagggctt cacgcagcgc tgcaggaata aatcgcgcat
    10381 cacgcagtgg ctgcaagcga gacattgcgc caatccgcag tgcaccgtta tcccagtcaa
    10441 cccagtccag ttccagatcc tgtaaggaaa tggcaatctt tttatcggta cgggttggtg
    10501 tagcattgag tttgctgccc ccggcgaacc agacggcttc atcctggtag cggcgcttca
    10561 gttccagcgc ctgttcgaca gagtcgggcc tgaaaaattg ttcaatcatc ttctttcctt
    10621 atttaaccct cttccctgcg ggaagagggc atctgtcatt tatgccagcg catccatccg
    10681 acgccacata ctggcagcgg cttttctggc ttgtgcataa atggaatcgc aatcgaagtt
    10741 aaactgacgg tcttcataga ccatcacacc attgaccatc acgctgtgaa cgctgcctga
    10801 gcccataccg aaagcgatat gcccggcgat attgtctgcc agcagcggcg tcggtgagtt
    10861 gtaatcgcaa atggttaaat cagctttgta accggcttcc agaagtccaa atttcgcgcc
    10921 aaagttgcgg ctcatcagtt cgttaccgtt agtcagggct ttggcaaaac tgtcaggcca
    10981 cagcggacca cccgcatcgc gatgtttaaa gaaggcaaat ttcatctctt caaacatgtc
    11041 cgaaccaatg ccgtccgttc ccaacgccag attgcggatg tcgctaaggt gatggttgta
    11101 gccgacatgg ttgttcatgt ttgaacgggc gttatgcacc aggaacgcat cgcgctgatt
    11161 gagtagggcg atgtcatctt tcgacaagta cagcccatga gcgaccagcg ttttgctatc
    11221 gatgagatcg aattgcgcca gtcgtgccag caggtctttg ccgtaccagt ggtgactgta
    11281 ggaaacgtcg taaaggtctt ccgcagcgtg aatatgcaaa ccacggcctg tggctttcac
    11341 ggcttcatgt agcatctcca gaccggcatc tggcacggta aacggcgcgt gagcaccgat
    11401 atgtgcttcc accagatacg gctcggttgc tgctttcttc gcctcatcaa tctgacgggc
    11461 gaagcggatg ttttcttcta caccttcctg caactctttg atgccgttgt tacggtcagt
    11521 agtttcaaaa caggtcatcg cgcgcaggcc aacttttaaa aatgcgtcgc gcaatgtgga
    11581 gagcgacccg ccgatatacg ccggagaggc atggtgatcg ataaccgatg tacatccgct
    11641 cttaatcgct tccagggaac aaatcagccc gctgtaatag agcgactctt catcaagggc
    11701 gcgatcgagc cgccaccaga gatttttcag cgttgagatg aaatccgggc agggggcgat
    11761 gtttgccata attccgcggg aaagccccga gtaaaaatgg ttgtgcgagc agacaattcc
    11821 cggcatcaca atccgaccat gcatctcttt gtagctggca tcggggtagc gttgcgtcag
    11881 ggcatcgccg atagcgacaa tcacatcatt ttcgatagcg atatcaacgc cttcctgcac
    11941 tttcgccggg tgtaactgca ctgcggtgac attcttcaga atcaacatga ttacacctcc
    12001 acgcggccca gcagataatg atgatgctgg tggacatggc tgatgatgcg gcacatatcg
    12061 ttcagctccg gtggtacgtt gttgaactga ccttcgctgt cgatgtttaa cacccagctt
    12121 tggttattca gtcgtactcg tacccggcaa tcttccacaa ggaagcctgg gttgctgctg
    12181 ttatcaaagt cttgcgccag gctgaaaacg gtgattttgt ctttgtatgg tttaccgttc
    12241 cacggacaga actgagcgca gttgccgcat tcgttacagt aagcgtcgag gtgcagcgtc
    12301 tggaatctat tctggaagcc tgggaccgca atggagacgt tggcgcggtt cgggcagaca
    12361 tccacacact tgctgcaaac gtagttacat tcgaggcagc gagcggcttc ctgggcgaca
    12421 aacgcgtcac gatcgtcact gttcaccaga gtgatagaga tatcgccttt acgttgatag
    12481 atttccgccg gattgacgtt gttccagtat ttatcgttct ggtgggaacg gatattttcc
    12541 cgactaagga tggcatcggt cgcccgacgc gcggttccga cagcagcgac aatggaggat
    12601 ggtccgcgct gtacgtcgcc gatcataaag acgtcagtca gacgagtttc gccattgtgg
    12661 tcgacgtctg gccagccgtt tttgtccagc ggcacgccca tcgcattcag ggcttcagta
    12721 tcctgctgtt caccaatggc ggtgatcagg ctgtctacat gcagtgttac tgtttcgttg
    12781 gtttcaaccg gacggcgacg gcctttctca tcgggttcgc caagcgacat aacgcgcaag
    12841 gttaaggtgc catcagcatc gaaacgttcc ggattattca ggaaacggaa ctccacgccg
    12901 tcgtgcaacg cttcttcata ctcttcgcgc catgcgggca tctcttgcag tgaacgacgg
    12961 taaacgatcg ttgctttttc tacgcctgga acgcgtaacg ccgcacgagc gcagtccatt
    13021 gcggtgttac ccgccccgac aacgaccaca tgtttgccca gcttgagcgc tgtacccttg
    13081 ttgtattcac gcaggaaggg gagtgatttc caaacatttt ggttgtcacc cgccagtttc
    13141 acaccgctat ttttatcagt gccggtggca atcagaacat agtggaagcc ctgatttttt
    13201 aactgttcaa cggtcaaatc gggtgagcag ccatactcaa atttcacgcc gtgatcggca
    13261 acaaaatcga tatcgtgctg aattaactca gccggaattc ggaactgagg aatgatattt
    13321 ttcaccacgc cgcccgcatt ggcttcgcgt tcaaacagcg taaccggatg gcccgctctg
    13381 gcaaggaagt aacctgctgc cagacccgcc ggacctgcac caatcacggc aaccggatgg
    13441 cgtgaaccag aaccggctgg tttgtgccag cgttgcttat attcatccca acctttttcc
    13501 agcgcgactt ttttcagttc gcggatattc agcgcactgt cgtaatccag gcgggtacag
    13561 ttgtattggc actggtgatc gcaaatatga ccggtaatgg cgggcagagc gttgcgttgg
    13621 tagatgagtt ccagcgcgtc ggcatagcgg tgttcgccaa gcagacggat gtattccgga
    13681 atatcttgct tgatagcgca ggcagtaaca cagggggcaa cgtagcaatc ggtcagcggc
    13741 aggtcttctg ccacttcaat acgctcttct ggcttccagt gtttctgggt gtattccatg
    13801 gttaacgcat ctgctgccag tctgttcagt cgttcgacgt caacatggtc aagtccccag
    13861 gcgtcggagc cttccagctc gcgcatgcag gcacttaagc gcaaatagcc gccaggtttc
    13921 agcaggtcgg ttgccatagt aatagggcga atacctgtat caaaaatatc gcggatagtc
    13981 agctgactgg caccaccgga ataagaaatg ggcagtttgc cgtcaaaggc gcgagagaga
    14041 actgctgcaa cattgatgga gagcgggaac agcgcacggc ctgacatata catctcttca
    14101 ccaggcagtg cgcctttatt gttgatggtg ccgagagtgt tagtcagttt tacgccaaag
    14161 ccgagtgatt tttcttttgc cagtgccatc aggcgttcca gcatttccag tgcttgcgtc
    14221 agcttgaggt cgtgatcaaa tgactcttct tttaagccta tgtaaccgaa accgcagaca
    14281 tcgaggatct cacgaacacg cgcgtatccc agtaaggtcg ggttaagttt cacaaaggtg
    14341 ttgagccctt tttcttccag catgtagcgg caaatggctt cgatttcatg cggaggacag
    14401 ccgtgcatgg tggagagggt gacgccctgc accatactgg tggggatgcg agcgggtaag
    14461 gcttgcaagc tttcgcgttt ttcctgcaat ccgtggcggg ctaaaaatgc gtcatcctgg
    14521 agtaatttat tcagcgtatc gcgatattgc gcgaatttcg gatggtcaga tgcgtccatc
    14581 atattgtcga tgaactgttg cattggcggt tgcttaatac cttcgaggtt gtaaccgacg
    14641 ctcatattaa agatgaacga tttaccggaa tcagaaggct ggaacatcgc ttcgagaagg
    14701 tgcagggcaa accaggcttt gaggtattca tcccaggctt taagcagggt aaattcggta
    14761 gaccattcgg tgttaaagca ctcgtcttcg gcatcgatac agggcttttc cagctccagg
    14821 cggtcaagaa tttggacggt ttttagttcg atgaagcgtc cgccagtcag ccaggaagtg
    14881 acaatatttt gcgcgagctg cgtgtgcggg ccagcggcag ggccgacggg agtggcacag
    14941 gtttcaccga agacgctaac agttttacct tttacaggtg agtaaaactg ttgctcggga
    15001 ataccaaaga ttgagcgttg ttgttggtat tcatcaaata tgcgcgtcaa aagttcctca
    15061 aacggaatgg gacgcataat atcccccata accctctcct tagcttatac agatatcatt
    15121 gagtgaccgg tgttgagaaa tccggtggtt tagggggata gagcaacaat cgcaccactc
    15181 ttgccaggtt tggcgggaaa tataaaaaaa tgtgaggcgg ctcgaaactt aaaggctttt
    15241 tgtgaatgaa ctcacaggtg ccaaatgaga attaaaatca ctcctgaaaa ttattattgt
    15301 cagcccattt atcacttttt ctcttttctc aaaatcagtg attccttatc attatgataa
    15361 agagtgctgg agagattaaa tatttgaacg caatcgttta actacatttt ctaaatttta
    15421 gtataccttt tttgcgctgt aataaagtcg tccggagtat caatatctaa aattatttct
    15481 gcattttcta tctcgacgga ataatgagct cccataagga gtgcctgacg catattggtg
    15541 acattgggtc gttggattgc ctgcatcaag catggttttg acactaaaat cggatggccg
    15601 gggatgccat tatggagtgg cagtattgcg ccatcatttc gtaacgacca gatttttcta
    15661 aaaatatcga tggtgagggt tggcatgtca ccgtgggtga gaaaacaatg ttctgtttgc
    15721 actgcgggta ctgcggcctt tactgatgtt agtaaaccct gcgcataatc tgggttgtgg
    15781 ataatagtga tattgctctg gttcgcataa cgttcgtgca gttcattacc acgatagccg
    15841 gtgactaaaa taattcggct acaaaactgc aacgcatttt tgatacttgt atcaagaatt
    15901 gtcccctgtt gccagggtaa catcattttc cattgcccca ttcttgatga taatccggca
    15961 gcggtaatta tacagtcgat ggctgacata gtggttccat tatttatagg ggctaataac
    16021 gcctgataat tgcgttactt cctgatggat taagttgggg ataatttatc ccagagaggt
    16081 cataaagact cgtgaaaagt atagttgacc catcggcatt agtcattgat ttaggtgcgc
    16141 agaaacgtcc cactgtgatt tctgttgttg gtgctggggg gaaaaccagc ttgctttttt
    16201 ggctggcaga actgctccag gctagcggca ggcgtgtgtt aatcacgacg acgacacata
    16261 tgtttatgcc aacgtctcac tggcccgtgg ttttctgtcg tgatcccgcc atgcttcctc
    16321 atgcgtctct tacatccccc atttcatttt gttttcacag ctggaaagcg aaccagggaa
    16381 aagtgcaggg atttacgcca gaagcgattg atgcactggt gcaacgacca gagtgtgacg
    16441 taattctcat tgaagcagat ggctcgcgtg gaatgccgtt aaaagcgcct gatgagcacg
    16501 aaccttgcat acccaaaagc agttgctgcg tgattgctgt gatgggagga catactttgg
    16561 gcgcgaaagt gagcacagaa aatgtccatc gctggtcgca gtttgctgat attaccgggt
    16621 taacacctga tgcaaccttg caactgagcg atctcgttgc gctggttcgc caccctcagg
    16681 gggcgtttaa aaacgtaccg caaggttgtc ggcgagtctg gttcattaac cgtttttctc
    16741 aatgtgagaa tgcgattgca caaagcgagc tccttcaacc gctgcaacaa cacgacgtag
    16801 aggcaatctg gctgggcgat atacaagaac atcctgcaat cgcgcgcaga tttgtgaatt
    16861 agcggcccgg gtattccggg ttactgaaag gatatccatt gaggttggta tgaatatttt
    16921 cacagaggct gcaaaactcg aagagcaaaa ttgtccgttt gcgatggcgc aaattgttga
    16981 tagccgaggc tcgactcccc gccattctgc acaaatgtta gtgcgcgccg atggttctat
    17041 cgtcggtaca attggtggcg gaatggttga gcggaaggtg attgaagagt cacttcaggc
    17101 attgcaggaa cgtaagccgc gattattcca tggacgtatg gctcgtaacg gtgcggatgc
    17161 tgtcgggtca gattgtggag gtgcaatgtc agtatttatc agcgtccatg gtatgcgtcc
    17221 acgtctggtg ttgattggcg cggggcatgt caaccgggcg atagcccaga gtgcggcgct
    17281 attaggattt gatattgccg ttgccgatat ttatcgcgaa agcctcaatc ctgaactatt
    17341 ccctccatca accacgcttc tccatgctga gtcgtttggt gcagcagtgg aagcactgga
    17401 tattcgccct gataattttg tcctgattgc cacgaataat caggatcgtg aagccctcga
    17461 caaactcatt gaacagccca ttgcatggtt ggggttgctg gcaagtcgcc gcaaggttca
    17521 gcttttcctg cgtcaattgc gtgagaaagg cgtggctgaa gaacatattg cccgtttaca
    17581 tgcgcccgtt ggttacaaca taggtgcgga aacgccgcag gagatcgcca tcagcgtgtt
    17641 ggcagaaata ttacaggtga aaaataacgc gccgggtggg ctgatgatga aaccttctca
    17701 tccttccgga caccagctgg tggtgattcg cggtgcggga gatatcgcca gtggtgtggc
    17761 gctacgtctg tatcatgcgg gttttaaagt gatcatgctg gaagtggaaa aaccgacagt
    17821 gattcgttgt acggtggcgt ttgcccaggc cgtgttcgat ggcgaaatga cggtcgaagg
    17881 cgtcactgct cgcctggcaa ccagctctgc ggaagcgatg aaacttaccg aacgcggatt
    17941 catccctgtg atggtagatc ccgcctgttc attgcttgat gaactgaaac cgctttgcgt
    18001 ggtggacgct attctggcga aacagaattt gggaacgcgg gcagatatgg caccagtaac
    18061 aatcgcgctt gggccgggct ttactgcagg gaaggattgt catgcggtaa ttgaaacaaa
    18121 tcgcgggcac tggctcggtc aggtgattta ctctggttgt gcgcaggaga ataccggtgt
    18181 tcctggcaat attatggggc ataccacccg acgggtgatc cgtgctcctg ctgcaggcat
    18241 tatgcgatcc aacgtgaaat taggcgatct ggtgaaagag ggcgatgtga ttgcctggat
    18301 tggtgagcat gaaattaaag caccgttgac ggggatggtg cgtggcttgt tgaacgacgg
    18361 cttggcagtg gttggtggtt ttaaaattgg tgatatcgat cctcgtggtg aaaccgctga
    18421 tttctccagc gtttctgata aagcccgggc gattggcggc ggcgtacttg aggcgttaat
    18481 gatgttgatg catcagggcg tgaaagcgac aaaagaagtg ctggaagtgg cttaaatgaa
    18541 aatggccgga tggtattacc cacccggcct taaaattact gacaaatcac cgttccggtt
    18601 ttgccttcaa tcccttcttt cgctttgctc aatacggtaa tcagtgcttc acgacctgcg
    18661 cgagagcgag caaacgaggc ggcagcttcc actttaggca gcatagaacc tttcgcaaaa
    18721 tgaccttctt cgataaagcg ttcagcatca cttaatgaca ggcgatcgag ccactgttca
    18781 ttctctttac cgaagttaat ggccactttt tctaccgcag tcagaatgat cagcatatcg
    18841 gcatcaatca tttctgctaa acgggcgctg gcccagtctt tatcgataac cgcgctggca
    18901 ccacgcagat ggttaccttc acgaataaca ggaataccac caccgccaac ggtgatcacc
    18961 acctggcctg catctaccag agctttaacc gtttcttttt caataatatc aaccggtttt
    19021 ggcgaggcaa caacacggcg atagccgcga ccagcatctt ctttcagagt gtaaccctgt
    19081 tttgtcagtt gctctgcttc ttgctcggta aagaacgaac cgatcggctt ggtcgggttg
    19141 aggaatgccg gatcgttagc gtcgacttcg acctgagtaa ccagcgttgc taccggttta
    19201 ttaatgcccc gagaaagcag ttcttctctt aaggcgtttt gtagatcgta accaatataa
    19261 ccctggctta aggcaacgca gacagacatc ggcagcattg gcgagtgcgc ttcagttttt
    19321 gcagcagctt caaaggcctg gttaatcatg cccacctgcg gcccgttacc atgagtgacg
    19381 ataacttcat gtccttgggc aattaaatca acaattgcct gagaagtaat ttttaccgct
    19441 ttcatttgtc cggccaggtc atcgcccagc gcattcccgc ccagggcgag aacaattttc
    19501 ttactcattt ttcctcactc atcattcttg attgtgtata gatccctgac agtggttaga
    19561 gcacgggagg gacaaacggt ttgcgtcgca ggaaacgacc tcgcccggct ttgcctgtaa
    19621 aggtgccgtc acagaaaatc gtttcaccac gggataaggt tctgacaatc gcgccctgac
    19681 aggtaaaacc ctcccatggc gagtagtcgg cgttgtcgtg gagatggcga tgctgaattt
    19741 gttggctctg acgtgggtcg ataatcacca cgtcgccatc ggaaccgggc gctaataatc
    19801 ctttttgcgg ccacaggcca aacagcctgg cgggcattgc gctggttaat tcaacaaagc
    19861 gttccggtgt tatacgtccc gtcatcacgc cactggaaaa cagtaactgc atgcggtttt
    19921 ccacaccggg taagccattt gggcagcgac tgaaatcgcc tttagaaatt tgcaggcgtt
    19981 gagccatcga gaaggtgcag tgatcggtcg ccaccacgtc aatcgcacca tcgctgatgc
    20041 cacaccacag tttgtcctgc tcgcgtacgt tacgcagcgg tgggctaaga atgaacttca
    20101 tgccgtcttc tgtatcgtaa ctgcgttcgt ccaacaggag atattgtggg caggtttcaa
    20161 cccagactgg ctggtgattc gcacgggcaa gacgcagata atccagacct aagccgttag
    20221 acaggtgcac gatatagagc ggggcgttac cggcaatttg tgccaggtta atcatgcggg
    20281 cgatggcttc cgcttcgcat tccagagggc gactcaaggc atgatagcgc ggcgcggtta
    20341 accctgcggc gataaattcc gcccgcttgc tggcgatagc cgcatcattt tccgggtgca
    20401 cggtggtcag cgcgccggat tcatgcagac ggcgtaatgc ctgcaaaacc tcgtcatcgt
    20461 tgagtttgta ttgataggtt aaatagagtt taaaactgct cagtccttcc tcgaccatca
    20521 tcgggatttc gtcgaggatt gcgtgattaa tgtgctggat cacaccgtga aagctgtaat
    20581 cgatgaccgc tttatgggcg gcataaccac gataaacctc cagttgatgg cgtaaccgac
    20641 agccgtttgg gccaaatccc atatggtcaa taatggttgt tgtaccgcca cacgcagctg
    20701 cgcgggtacc ggtaaaaaaa tcatcacaac tgcgcgcgat gccgacatca atattgaaat
    20761 gcgtatggac atccacgccg ccagggaaaa cgtaacagcc agtggcatca atttcttcat
    20821 acgggagctg cggcgaaata ttgttgccca actggcgaac aatcccgctt tcaatcagca
    20881 aatcctgttt ggcttgtcca tctgcgttaa cgacagtgcc gtttttgatc aatacgcgca
    20941 tagcaaactc caaaaaccct ccggcgaacc ggaggggaat aagtgaagag taattactct
    21001 gttgccagcc agcttaacgg gattgcagcg tacatcgcgg cacaggtcac caggtgagat
    21061 ttccaggttt tttcgttagg tgcatgcgct tcaggttctt tacccgggcc aaagccgatc
    21121 accggaatgc cgtgacggcc catgatagat acgccgttag ttgagaaggt ccacttatca
    21181 acaaccggcg ctttgccaaa cagcccttcg taggcattca ccagtgcttt aacggtgaag
    21241 tgatcttctt ccactttcca ggtcgggaag tagcattcgg ttgggtaaac caggccagtc
    21301 caggacggac ggtcgtagtt gtacatagaa acaaccgcgt tagctttctg tactgcaggc
    21361 agggcgcgga tttcgtccag cgcgccttcc caggtttcgc cccaggtcag acggcggtca
    21421 atagagacgg cgcagctatc tgctacagcg caacggcttg gggatgtgaa gaagatttca
    21481 gaaacggtga gggtgccttt gccgaggaat tcgtcataac ccagacgttg ggagagttct
    21541 tgtaattcgc caagaatcgg acccattttg aaaatggcgt tgtcaccgcg ttctggcgca
    21601 gaaccgtggc agctaacacc ctgaacatca atacgaattt ccatacgacc gcgctgacca
    21661 cggtatacct ggcagtcggt tggttcggta ctgaccacaa attccgggcg aatgccggat
    21721 tgttcaataa tgtactgcca gcacagaccg tcgcagtctt cttcctgcac agtaccggta
    21781 accagcaggg tatattcatc ttccagaccg aggtctttaa tgattttacc ggcataaacc
    21841 atagatgcca tgccgccttc ctggtcggaa gtaccgcgac caccgatcag ctcatcagtt
    21901 tccatgcctt cgtacggatc gaagtcccag tttttgatgt tgccaatgcc gacggtatcg
    21961 atatgagcgt ccattgccac cagacgcggg ccgtggccga tataaccgag aacgttgccc
    22021 atcgggtcga tttcaacttt atcgaagccg actttttcca tctcttcttt aatacgatgt
    22081 actacgcgtt tttcgtcgca gctttcactg ggaatagcaa ccatgtcgcg caggaagcga
    22141 gtcatgtccg cctggtaatc ttttgctttt tcaagaatca gtttgaatgg aatattctta
    22201 gccattattt ttctccagat tttttactcc ccgttttgca gggagtagtt ccaatagggt
    22261 gattaaggtg ctacagcgtg tttgccttcc cagacaactt cgcggtagtg cttcacgtcg
    22321 gtgtcgcctt cagtgctgat aaccagcacc acggcatctt tgttcagcgc cagtttttcc
    22381 atcaggcttt gacgttgcgg gtgataatga accgctgcga gaacgcccaa accgacagcg
    22441 ccggattcac cggagatgat gcgcgggtcg ttgccgtacg gattacccag cacgcgcata
    22501 cctaatgcgg caacgctgtc ctggcaggag atgaattggg tggcgcagtt acgtaggatt
    22561 tcccagccca gcgggttagg ttcgccacag gccaggcccg ccatgatggt ggccatatca
    22621 ccgccaacgt tgacgatgtc gcctttgacg ccggagcgat aaatacagtc agctttgtca
    22681 ggttcaacaa taatgctgtg cagattttgc gggctgtaga cgtcgaccag ataacccagc
    22741 acaccaccgg ccattgctcc gacaccggct tgcagcagaa cgtgcgtcgg ggttacgccc
    22801 atttcacgca tttgctcgac ggcttcatct gccagggttg cgtagccttg catgatccag
    22861 gttgggattt tggtgtaacc ttcccatgcc gtgtcctgta ccacttccca gccgtgctgc
    22921 tgcgcgtgtt gcatggtcag gcgaacggta tcgtcatagt tcatatccgt gacgatgcac
    22981 tcggcaccga ggttcagaat ggcgtcaacg cgttcctgag cagaaccttt cggcatgtaa
    23041 atcaccgcat tctgtccgag ttgctgtgct gcccacgcca caccgcgccc gtggttgccg
    23101 tcggtggtgg tcgcgaaagt cattttttcg ccgatggcat ttttcaggtg ctcaaatgac
    23161 agcgtttcga tatcaagatg atatttttca cacaataatt gagcgatggc gtacgcaccg
    23221 ccaagcattt tgaaggcgtt cagaccgaat cgttttgatt cgtctttgac gagaattttc
    23281 ttcacaccaa aaaggtttgc gagatcgtcc agcgcacaaa gcggtgttgg gcgataacca
    23341 gctattttct ggtggaactg gcgcgccagt ttggcctggc tttgcgaaaa gagcgatgat
    23401 gtttcgccgt tgaaaaattt gttatcggcg atatcaatct tcaatgagaa aacggacata
    23461 tcctatcctt ttggcctctg gaaatagagg gccgaccagc tgatcggccc gaggggttat
    23521 ttcacgcgtt cttgcgcttc tttcagcagt tgctcgagca gtgcacctgg tttggcgtat
    23581 ttacgggaca ggatcatcgc ggcgatgata taaggcttcc agctggcttc tttgtaggta
    23641 gcgatacggt atttttcgaa tacgccttca gtcacttccc cttctttgca ggatacgccg
    23701 ctgatatcag ctggcaggca gtgcatgtac agggcttcgc catcacgggt cagttccatc
    23761 atctcttcag tacaatgcca gtctttgtgt tgcgcgttct gtgccagaca ctgtttttcc
    23821 agtgctttta agccttcgtg atcgttagcg cgcagcaatt cggtacgctg ttccatcact
    23881 ttgtaaggtg cccatgactt cggataaacg atgtctgcat ctttgaatgc ttcttccatg
    23941 ctggtgacct gacggaagct accgccagat gctttagcat tgtttttcgc gacttctact
    24001 acatccggga tcaggtcgta gccttccgga tgggccaggg tgacatccat accgaagcga
    24061 gtcatcagac cgatgatgcc ttgtggtaca gagagcggtt tgccatagct tggagagtag
    24121 gcccaggtca tggcgatttt tttacctttc aggttttcca gtgaaccaaa gtgttcacgt
    24181 aaccacgcga ggtcagccat tgactgagtc gggtggtcaa tatcgcattg caggttcact
    24241 aaagccggac gctgtggcag tacaccctgc ttgtaaccgt catcaagtgc agcgccaact
    24301 tcacgcatat aggcgttgcc tgcgcccaga tacatatcgt cgcgaatacc aatagcgtcg
    24361 gcgcagaagg agatcatatt ggcggtttca cgcacggttt cgccgtgagc gatttgtgat
    24421 ttgccttcgt cgagatcttg ttgtgcaagg ccgagcaggt taagcgcgga agcataagag
    24481 aagcgggtac gagtggagtt gtcgcggaat acggaaatac ctaatccact attaaagact
    24541 ttggttgaga tgttttcagc acgcagtgct tttaatgctg cggcaacgtc cagtacttgt
    24601 ttcagttcat ctggcgtctg ttcccacgtt aacaaaaaat ctttctcgtg aaggtgagag
    24661 gtcagcgaat tgatatcctt aatcagctca ttaacagttt tcatcataaa gtcccggtag
    24721 tggaatgaca ggcaaagtgg tttatttcac tgtgacttgc caaagtgatt ggtgttttca
    24781 cagctatacc tgggactaac aataagtgtg ccagaattat ccacggggct gtaaaagact
    24841 aatttgacgg ataatgtgtg gggttgatca caaattgatg ccagcttcgg gttgtttgta
    24901 aaatacgaaa acgattggct tttcttatcg cgttatttat ttactgctaa gtatcagaaa
    24961 taagataaag ggacaggcta tctctcttca gtttgataaa ctcaccgccg taattctcat
    25021 attgatatag aaatgtgata tctcttccat aatttcatta gaaagtgata aaaatcttat
    25081 gtttttatca aagccaattt cgcctgagtt aatatcatca tgatctgtga accatcaacg
    25141 tcttcaatga tcgactttta agtaagtctg gaaggtaaag ctatggagct tgctactacg
    25201 cagtcagtat tgatgcaaat tcaaccgaca attcagcgtt ttgccagaat gcttgccagc
    25261 gttttgcagg ttgagatcgt tgatgaaaac ttgtgtcgcg tcgccggaac tggcgcgtat
    25321 gggaagtttc ttggccgcca gttgagcggc aactcacgcc tgctccgcca cgtcctggaa
    25381 acgaaaactg aaaaagttgt gacacagtct cgcttcgatc ccctttgcga aggttgcgat
    25441 agtaaagaaa attgccgcga aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt
    25501 tgtgttgggg tgataagttt gattgccgtt acccacgagc aacaagagca tatcagtgat
    25561 aatttacgcg aattttctga ttatgttcgc catatatcca ccatttttgt ttcgaaactt
    25621 ctggaggatc aggggccagg agataacatc agtaaaatat tcgcgaccat gatcgataat
    25681 atggatcagg gcgtattagt tgttgatgat gaaagtcggg ttcagtttgt taatcagact
    25741 gccttaaaaa cacttggtgt tgtacaaaat aatattattg ggaaacctat ccgtttcaga
    25801 ccattaacat ttgagagtaa ttttactcat ggacatatgc agcatattgt ttcgtgggac
    25861 gataaaagtg aattaatcat tggtcaattg cataacattc agggccgaca attattttta
    25921 atggcatttc accaatcgca taccagtttt tctgtagcaa atgcacctga tgaaccacat
    25981 attgaacaat tggttggcga gtgccgtgtt atgcggcaat taaaacgact cattagccgt
    26041 attgcaccca gcccatccag cgttatggtg gttggtgaaa gcggcacggg taaagaagtc
    26101 gtcgcccgag caatccataa gttgagcgga agacggaata aaccctttat tgctatcaac
    26161 tgtgccgcga ttccggagca gcttctggaa agcgaactgt tcggttatgt taaaggcgca
    26221 tttactggcg cttctgccaa cggtaaaaca gggttgattc aggcggcgaa tacgggcacg
    26281 ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc aggctaaatt actgcgcgct
    26341 attgaggcgc gtgaaattct gccgattggt gccagtagcc caatacaagt cgacattcgc
    26401 atcatttctg caactaatca gaatttggcc cagttcattg ccgaaggtaa attccgcgaa
    26461 gatctcttct accgacttaa tgttatcccg ataactctgc caccgctgcg tgaacgtcag
    26521 gaagatattg aactattggt gcattacttt ttacatctgc atacccgtcg tctgggatcg
    26581 gtttatcctg gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga
    26641 aacctgcgcg agttaagcaa tttgatggaa tatctggtta acgtagttcc ttcaggtgaa
    26701 gttatcgaca gcacgctatt gccgccaaat ctgctgaata atggcacaac ggagcaaagt
    26761 gatgtaacag aggtcagtga ggcgcacctg tcactcgatg atgcgggcgg cacggcgctg
    26821 gaggagatgg aaaagcaaat gatccgcgag gcgctttcac gtcataacag caagaagcaa
    26881 gttgctgatg aactgggcat cggcattgct acgctctatc gcaagattaa gaaatatgag
    26941 ttgttaaaca cataacctca gtgaatccgg cctgaattca ggccggatat ccttttactt
    27001 cgttttctcg caatccagaa ctgtatttac aatcatctga taccccgtgc agcgacaaag
    27061 atttcccgcc agtccgcgac gaatttccgt aatggttaat ggcttctcgc gtggtttcgc
    27121 cagcattgcc gtggtagcca taatcaggcc aggcgtacaa aacccgcact gcactgcgcc
    27181 ggatttcgca taagcctgct gaacatgaga aagttttccg cctttcgctt caccttccag
    27241 cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat aagcaactgt ctattgctgt
    27301 gccgtcgacc agcaccgtac aggcaccaca ttcacccacg cagcaccctt gtttgacact
    27361 tagcagtcct tgttcgcgga gtaattccga gagcggcgtg cctggtgcgg cgtgaagctg
    27421 aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat tcattgcaat
    27481 tttccccccg ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt ctggatgaga
    27541 tgcagacgaa actctttact ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca
    27601 gattcgctga tagcttccag cgtttgcagg tttaatggcg cattttgtgc agtctgttcg
    27661 gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg taattcgctg
    27721 aaattgccgt tatccagtcg gcaatgtgcg gcgcagccaa tcgttgaaat atccattgcg
    27781 tcgcgcatgg catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa
    27841 tgaaaggcga cgaggatttc gtcatgctca agagacactt tgcccggccc ggtgtgaaag
    27901 ccattaatcg ggacgaaacg aacaccgcgc ggggagtgga tctccagttt cgcgtcataa
    27961 attagcgttg gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag
    28021 gtagcgacgt tacggatctg cggtccagca atggacgtgg ccgcagcaca taacgccggg
    28081 agatgacgtt gagttatagg atcttctatt agctgggtaa atgtcgttgc agagccgata
    28141 cgtagcgagc catcttccgc cagcgtaatt ccccgcagct ccgccagatt atgaatatca
    28201 acaatatggc gataacggtc attgtgatgg tggagctgaa tcagtacgtc agtgccaccg
    28261 gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga tggcatcggc aagggttgct
    28321 gcgcgatggt aagaagcaaa atcaaacatg atgttatcct caaatcaatc ctgccagatg
    28381 gaactcttca tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa caccggtagc
    28441 catcttcacc gcgttacgaa tagcagcggc aacaggaatt attggtggct cacccagtga
    28501 cttatgtccg tatgcggatt gcggcttatt gatttcgacg aacgcgcttt ccagttgtgg
    28561 cagatccggc atggtcggca ttttgtaatc cagcagattg gggttacgga ccacgccgct
    28621 tttagcatcg atgatcatct cttcaaatag cgcccagcca atgcccattc ccattccgcc
    28681 gtgtacctga ccttctgcca gcagtggatt aagaatatgc cctgaatcat gaacgttgag
    28741 gatgcggttg atggtgactt tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca
    28801 gccaaacgcc ggtgggttag tggtggtttt gatggagctt tcagcagaga gctgcccgcc
    28861 gcgttcaggg tggtagaaag cgtccatcgc caaatctttt aacgacatta acggctcttc
    28921 cggtcgttca accagcacga tatggccttt tatcagggtc agattcatcg ctgactgatg
    28981 tagcatgact gcggcgtgag cgatgatttt ctcttttaat aatagtgccg cactgcgcag
    29041 cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg ccgggatcga acggcgtaac
    29101 gtcggtatct tgagttgaaa taacgcgaac gtcgctgacc ggaaccccca cggtttctgc
    29161 caccatttgc gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac
    29221 gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct gctatttcta cgccgacagg
    29281 ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg ccgcggcgca aattgccttg
    29341 ctggttctgg cattctgcac ggcgtttttc ccattcaaag attttccggc ctttttcaag
    29401 acactccggc aaccctgcgc tgtaaatacg tttgcccgtg agcggattag catctccttc
    29461 gcgggcggcg ttgcgtaaac gaatttcaac aggatcaata cctaacgctg tcgcggcgtc
    29521 atcaagcata gactcaacgg caaatacgac ttgtggcgcg ccataaccac gcatcgcacc
    29581 agccgagggg aggttggtat agcaggtctt tgaactgtaa gcgtaggcac aacgaggata
    29641 aaggtaagcg actttattcc cccccgcaga agcgatggag tgcccgtgag atgcataagc
    29701 gccggtgtta gacagaacat ccagactata acctttcaat gttccgtcgc ggttcacgcc
    29761 catttgcccg tcaatggtaa aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg
    29821 gctaagggaa actttcaccg gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg
    29881 ctcttccagt acatcctgtt tattaccaaa accgccaccg acaaacggtt tgatgactcg
    29941 tacgcatgac cagggaatat ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat
    30001 ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc atccacgcca gcgatgttac
    30061 gctttccatg tgacaatgtt gaataacggg ggtctgatag tgcccctgta cctggtagtc
    30121 ggcggcatcg attgtttgtt ggacattacc cgtcgacatc gtgctttgtt tcagtaaatt
    30181 gccaccgtta tggattggtg cagcgtcttc tgccagcgcc gcttctggcg tggtgataac
    30241 gggtaattct tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc
    30301 gcgggccacg acgatggcaa cggcgtcacc atgatgacga acatggcgag ttagcagtgc
    30361 gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccag gcatgcccag cagtagcaaa
    30421 tggaatatca ggcacatctt cccaggtaaa aatcgccagt acgcccggca aacttctggc
    30481 ttgttcatca ttaatactta cggcataacc atgtgcgata gggctacgta catatttcgc
    30541 ataacacatg cccgccataa cataatcgtc agtatatcgt gcccgcccgg tgaccttagc
    30601 aatggcatcg acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg cttccataga
    30661 aatcccctcg atttgaacat ttggttctta tggcaaagat ttagtctaag caggcaagat
    30721 ttacgccaga aacgacgata agcgttaggg gaggtaggaa gtgtgatgac gttaaaaaaa
    30781 cacagatatg atacgaaaca ggactctgaa taggggcagt gatacgtatc aatatgagtg
    30841 gcgataaaat caatgtgata aattgggatt attacgcatc aggatgggaa atagggtaaa
    30901 aagtcaggaa gttcgatacc tctacagcga atattttgct gatgatactt ccctttgcca
    30961 aatgaaatta acctctttct ctattaccac gtttttccag aagcaagaga ttgggtatca
    31021 agaggctggc tgctatgata aggcgtcttg ttttttaagc gaggaaagat tttgagtgcg
    31081 ggacgcctga ataaaaaatc tctgggtatc gtgatgttgt tatcggttgg actgcttttg
    31141 gcgggctgtt cgggtagcaa atcatccgat acaggaacgt attccggctc cgtttacacc
    31201 gtgaaacggg gggatacgct atatcgtatt tcgcgcacca cgggaaccag cgtaaaagaa
    31261 ctggcgcgac tgaacggcat ttccccccct tacaccattg aagttggtca gaaactaaaa
    31321 ctgggtgggg cgaaaagtag cagtagtaca cgtaaatcaa ccgccaaatc aacgaccaaa
    31381 accgcatcgg ttacaccgtc atcagcggta ccgaaatctt cctggccgcc agtagggcaa
    31441 cgttgttggt tatggccaac gacagggaaa gttatcatgc cgtattcgac agcagatggc
    31501 ggcaataaag ggattgatat ctcagctcca cggggcacac ctatttacgc cgcgggtgca
    31561 ggaaaggtgg tgtatgtggg caaccagctg cgtggctacg gtaatctcat catgattaaa
    31621 cacagtgaag attacattac ggcttacgcc cataatgaca cgatgctggt aaataatggg
    31681 caaagcgtga aggctgggca aaaaatcgcc actatgggga gcacggatgc ggcatctgtt
    31741 cgcctgcatt tccagattcg ttaccgtgca acggcaattg atccgctacg ttacttgccg
    31801 ccgcagggca gcaagccaaa atgctgatgg cgaattaatc agcagtcagc agcgcggcac
    31861 ttgctaagga gagcgtaagg tttataatgc cttacgcatc tcgaagcggg cgtagttcaa
    31921 tggtagaacg agagcttccc aagctctata cgagggttcg attcccttcg cccgctccaa
    31981 tttatcttca cctgatcaat ctgccatttt tgctgtatct gattttctat attatcttaa
    32041 ttcaatcgct tcagactatt cgcaatcgcc gtaatacttc cccattttgt tgtaaataaa
    32101 aagcgtttac gcaatgcaat ccttttgtaa caaccctggt aaaacacctt attttcaagt
    32161 tgttaaataa gtggttatct acgcttttaa caaacgattg atggaatatt ttatgaaagg
    32221 gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac
    32281 aggggcagaa gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac
    32341 a
//
LOCUS       DAZFWP020000042        32286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFWP020000042 DAZFWP020000000
VERSION     DAZFWP020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.042"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(16..201)
                     /locus_tag="ACHK0Z_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /pseudo
     CDS             complement(16..201)
                     /locus_tag="ACHK0Z_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
     gene            complement(258..596)
                     /locus_tag="ACHK0Z_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             complement(258..596)
                     /locus_tag="ACHK0Z_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4501839.1"
                     /translation="MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSAL
                     VNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLWEVENDMRVQEELSRINTAEKFI
                     SQRNLNKKAA"
     gene            complement(667..969)
                     /locus_tag="ACHK0Z_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             complement(667..969)
                     /locus_tag="ACHK0Z_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4501840.1"
                     /translation="MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIIN
                     AATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYRLIFKWVNGKAEDLYLDPHKY"
     gene            1132..1923
                     /locus_tag="ACHK0Z_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             1132..1923
                     /locus_tag="ACHK0Z_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4501841.1"
                     /translation="MGRFDEWFADDANLASKESEHDSTQRETPAPIEANAGGAPEHGN
                     TGSDSFSILEEQPAAKADSHDNFADSNGGLTPDAGESDIAILPPSLAGREPTPQLKAR
                     YNGHKAFNDQIRADWMLIIESSPDAFQALLYRPDVGTYGVVSDETGEESFTELDNNQR
                     ELTYQEPEIVYVLDNPDGRDSFHAIDADGEQDGLTDDVLILRIAANNVPVGSILEWNE
                     EMVNGVARRWWYVHRIFSYGTQHVGSLYYCIPARNFDTTQNGVIE"
     gene            1920..2687
                     /locus_tag="ACHK0Z_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             1920..2687
                     /locus_tag="ACHK0Z_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HGU4501842.1"
                     /translation="MTSNKTLLARTGEWQTLRTGKFQTTGFENVDNAFATLISNIFSD
                     ILLVAPAPEEKRFASFLNRPPAERVYMAHFDNAIEFLKAVRRANAGQGRKPENQNINR
                     DALPLVNISRTMDINYINDDQQIDRKKVASFCEPDTGTPLAELEYTQAILTYDVTLMA
                     TDKATMSLMCNSLGARLRLMTGTQFEAATHLVRVPVPLICSIQDAKEVGFTDVSAPIG
                     EERIYATQAPISVIADVITAWELDAKRIITETSISMG"
     gene            2691..3671
                     /locus_tag="ACHK0Z_RS09055"
                     /old_locus_tag="pgaptmp_001811"
     CDS             2691..3671
                     /locus_tag="ACHK0Z_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail length tape measure protein"
                     /protein_id="HGU4501843.1"
                     /translation="MAQELQQYFLQSVLINDNKVPRDWIFTAVYVEKTSLKAPLLKLE
                     IHDATGTVIDDWKAKYGASLVAEMGDPNGNAGTFKTDFFVTSAMLAGDVVTVIAVSED
                     VRRFKIPSPRTNLHTNKTPDAIFKAYSGKLKITSSVLKRAVTYHLNAGDKPSKMLSEI
                     ARDKGALCWVCRGEFNFYTLADLMKQTPSFTYEGNNPKAEYTLSKMRLLQQEHATTAK
                     NQYRFVGYSMTDGYVEYGDSSLPVRYISDSDMETLRNMQLSLVPKMDIEVAGNPDIKP
                     GMVIEIIVYRYDQENRIDESMPRKLIVKNVAHFEDRVGYTTRMILGVPNK"
     gene            3668..4321
                     /locus_tag="ACHK0Z_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             3668..4321
                     /locus_tag="ACHK0Z_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGU4501844.1"
                     /translation="MKRRAQIVGTVHPAGLMRAQVRVLPDWNGVPDDDLPWAEYQLPI
                     GNAFVPTVKGDLVWVEFPYLDVNGRIDTRRPMIVGAAQDAPGGIPNVAPEASGKGNGW
                     TPPEVDGAPPRPQISATKDFVIHRNNILEVRTAGGGYEIANTAAGSRIGMNESGQIYI
                     IGPADVIVNAGGSVNVKSANNINVNGENIAVTANGDIAFKAGGTFQATASNFDFKKG"
     gene            4381..5286
                     /gene="rdgC"
                     /locus_tag="ACHK0Z_RS09065"
                     /old_locus_tag="pgaptmp_001813"
     CDS             4381..5286
                     /gene="rdgC"
                     /locus_tag="ACHK0Z_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009444180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4501845.1"
                     /translation="MRSVAFKNIFIYRLSREVSWDTAEVNAALSKFVFTPCGSQDMAK
                     AGWTPVLGDNLTHEYQGFLLIQHKREEKILPSQVLKEELQKKILTLEEEQDRKLKKAE
                     KDSLKDEVLHSLLPRAFTRKSVAKILIDRSNHLVFVEASSAKKAEDQLALLRKSLGSL
                     PVVPFSPKDPLELTITGWIKNGFPNGFRAGEEATLKALLDDGGAVRCKKVDLLSDEIM
                     SHIEAGKVATTVAVNWQDRAFFRLNDDMSIKALSFSDELYDQNDDIDREDVAQRFDAD
                     FILFTAELSAMFTSLVEAVGGEAER"
     gene            5300..5950
                     /locus_tag="ACHK0Z_RS09070"
                     /old_locus_tag="pgaptmp_001814"
     CDS             5300..5950
                     /locus_tag="ACHK0Z_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501846.1"
                     /translation="MVSLCDFTGKMVAPWLEHGIDAVIVDPQHAETRDELMQSGAVLT
                     RISAIIDSDEVYAFLRNNLQRIVFLAGFPPCTDLAVSGARWFAHKADKDPFFQFKAMQ
                     VVWQCYDIAKMIGCPYMIENPVSQISTFWRKPDHIFHPYHFTAYCKEDNYTKKTCLWS
                     GQGFVMPDALMDESLGKPDNRIHTAPPGPERANFRSATPHGFAKAVFEANKGVLYE"
     gene            6030..6848
                     /locus_tag="ACHK0Z_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /pseudo
     CDS             6030..6848
                     /locus_tag="ACHK0Z_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(6897..8558)
                     /gene="glnS"
                     /locus_tag="ACHK0Z_RS09080"
                     /old_locus_tag="pgaptmp_001816"
     CDS             complement(6897..8558)
                     /gene="glnS"
                     /locus_tag="ACHK0Z_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4501847.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNIRYSSD
                     YFDKLHAYAVELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNQ
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KEAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKLG"
     gene            complement(8827..9114)
                     /locus_tag="ACHK0Z_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             complement(8827..9114)
                     /locus_tag="ACHK0Z_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501848.1"
                     /translation="MIKPRKSVKAPEVKDPDLERRIEDFASKADLVPGEQPEDNKVLD
                     KDAPRDFKSIRVGFNEYEYQVLDALSKKHNRSKLNMIRHAILMLAESEEAK"
     gene            complement(9107..9748)
                     /locus_tag="ACHK0Z_RS09090"
                     /old_locus_tag="pgaptmp_001818"
     CDS             complement(9107..9748)
                     /locus_tag="ACHK0Z_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000585023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4501849.1"
                     /translation="MIILVVSQKGGCGKSTTSVNICAELARANKDVVLLDADKQGTAA
                     RWAADRNTAEVSPVIHCVQKFGNIRETLLDLDKRYEFVVVDTAGRDSKEMRTGITAAD
                     IVLVPFRPSQPDLDTLAHFVEVFEEALDLMPNPSIKAFAVLTMAPSNPVVNETNEAKE
                     YLAEYPQLKLLKTIIRDRKVYRDCMAEGKGVVEMDNGKAKGEIQMLVKELLSD"
     gene            complement(10036..10374)
                     /locus_tag="ACHK0Z_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     CDS             complement(10036..10374)
                     /locus_tag="ACHK0Z_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4501850.1"
                     /translation="MSQERKKVMIYLRPEAYANEKAASEKIKKHSDMARTALLAGLAL
                     GEVDSRLPGLLASLLTEDNNPELIRKMLASFLELPAAVEERPASIEPVKEQFVAKSAS
                     ARNLADSLPD"
     gene            complement(10387..11343)
                     /gene="parM"
                     /locus_tag="ACHK0Z_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             complement(10387..11343)
                     /gene="parM"
                     /locus_tag="ACHK0Z_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HGU4501851.1"
                     /translation="MRIFIDDGSTNIKMLWEHDGETRTHISPNSFKRGWSATFGAGKP
                     FNYVIDDEKYSYDLISPDVLPTNNVEWQYSPLNVLAVHHALLTSGIEPQEVEIVVTLP
                     LAEFYDDDAQYNLDNIERKKASLMRPVTLNKGNVFTIKKVTVRPESIPAGISLCDNLN
                     PAHSVLIVDLGGTTLDVSMVAGQMTAVSRVFGDSNLGVSLVTREVRQALARANTETSN
                     YNVDQLIINRHDEDYLNDNINDPSAIGDVKKAIAASIDRLRTRVLDVIGDFKGYTHVM
                     VIGGGAPLVADAIRVQVNIRDDRFFVADDPQLALVHGLKAIG"
     gene            11610..11894
                     /locus_tag="ACHK0Z_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     CDS             11610..11894
                     /locus_tag="ACHK0Z_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4501852.1"
                     /translation="MSAIATSDTLCSGHGGFPPRLVAETVPWFTVNGKPVVVDGAMFP
                     SHTDGNSAHPGAAVSTRVWFSIGGKGVVCVGDPLSCGSVIANGEDIFQVA"
     gene            11894..12700
                     /locus_tag="ACHK0Z_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     CDS             11894..12700
                     /locus_tag="ACHK0Z_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501853.1"
                     /translation="MLDSIGEVKAFKILSDAGISTPESITISRAASVKASSLASAIQG
                     IVHADKTYPDSVSAWTTQLLGFSEQLNEASKASSLLADSLSPYTKPSELLQMKIGWEC
                     YAKGNELTPIPAFALVEGMGNVSIPQSLTDALTALKLDALKTAMNAINAKIEAAGSAG
                     GGESDGGQGGAGGAQAPVITQDEIDALREAVTAAEVLLSEINSASEGVVALTGRIKTS
                     TTQATKGLENAVAITLTGSLLDNAVMSPAISLIMPQGVIDALQKNTKKEP"
     gene            12771..13229
                     /locus_tag="ACHK0Z_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     CDS             12771..13229
                     /locus_tag="ACHK0Z_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501854.1"
                     /translation="MVLKKIILIVIALLAISGIYRTTTAVIEHIRYVRSLEDSKSQLE
                     STNNTLSTALQLSENARSAMLTENERIKALEKEYQRKTAELNANLIKQREESQNEITR
                     LENALRRAGINDVRVPDDVIRMQRERAKAINQRASENYRRSHQQAAGKSD"
     gene            complement(13383..13841)
                     /locus_tag="ACHK0Z_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     CDS             complement(13383..13841)
                     /locus_tag="ACHK0Z_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501855.1"
                     /translation="MSHVNPSKTQYRLMLSIASAIPTSLNPPAGYPAVVDDCFQYYGE
                     DILSQSKALKQLCKAGILHCIGDPDDFVVMLADRDSFLLSWKAGAREARLGNGIGYID
                     YSDCPLAFAGGYMHWHERNRGRQRQYRLSDFNVCHGFEEADSQDIWLQEP"
     gene            complement(13858..14250)
                     /locus_tag="ACHK0Z_RS09125"
                     /old_locus_tag="pgaptmp_001825"
     CDS             complement(13858..14250)
                     /locus_tag="ACHK0Z_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501856.1"
                     /translation="MAYRIFVSYKNGAKSHSLNTTSRFLVEAQLASILAESEILSLAE
                     RIVIQFSGRDILNVPALTPASEVMESIKWPVCGCPARVEEPVTATLYMPKAVRDWLAM
                     VGNGKVSAGLRKLIEMADIPELKNAWRQ"
     gene            14409..16106
                     /locus_tag="ACHK0Z_RS09130"
                     /old_locus_tag="pgaptmp_001826"
     CDS             14409..16106
                     /locus_tag="ACHK0Z_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HGU4501857.1"
                     /translation="MNKNIQPARDREREIMNRAASVMAMTVDPTTDAAGNMIADQAVM
                     MENLDKAIQKVPMFEGVNPEVARQITGGWAMSLHEYKRQHGHYPASDILANAHMALER
                     LMTECASDTHEGTGKAMFESVAQSMRSSDGVMKVAQYAALILPASLGAATSDACTFVP
                     CDRDESNIYELVNVAGTKFGTFKQGDELNMQSAGVYSQMKRLYTLSTKGDGSTKTFQF
                     DIQNFEGQSCPLRAGYNKLLINRKPSKVDDGDGNLYFNAKDSKGNAFSATAKVAYDTG
                     VIDITFTQAPPEGTEIAVQVEINIERNPSLIPVINQAMRKYEVRPSQYVIASEHTVMS
                     ASDLSREHGLELAALQFSAMRNWISHETDIMRLRTLVFHTVYGREFDVALPEAQNYES
                     WVGLLRHVVNALSQEMANRTLTTGIRGGFAGGDAANFLRSLPPQHFQIAPGYVQSPYV
                     QYIGTLFGTIRIYEVPQPVCEQFQAQGYDFGLDDIFFYGRGEGIGKAGLIAGDAVPAI
                     PYVHETNPSLVNRTTLWGSAINEVHPRNGENYFTRLRLTRAKEGAIDMLTGKVNEKK"
     gene            16254..16532
                     /locus_tag="ACHK0Z_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             16254..16532
                     /locus_tag="ACHK0Z_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HGU4501858.1"
                     /translation="MANIQVTITPSDATDKSFTVESDHPEIVQVDGTTCTALKAGQAV
                     LTIKTNDGNKTAQCTVTVREAPKSVSAVSVEPTTKEVTVGDNFTVSES"
     gene            16600..18258
                     /locus_tag="ACHK0Z_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     CDS             16600..18258
                     /locus_tag="ACHK0Z_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4501859.1"
                     /translation="MNKISFSVGQAAGVAVMSVNADATLTNTSGGASVFAGLVISRRG
                     APGKVLKVDDTTYQSVLGSPIHPRQGAAFEPYRHVERAVKGGSGYVVRVCAKDMKVPG
                     ISVSVVGKAKAAKASKELSVEPTETTVNPGDTLSVGVASTATTQSVSFTPKETPQIKG
                     EEKALFFIKDGDASQNRTLSLTRDDEESELFTLTLKEKQTDGSIEVLESHQVSFNPEG
                     TNDMGQPAWIPTLLESQSTRIGAVLADNAEASAAQLIFEDVAFEGGTDGDLSEIDTED
                     YLEALKVLEASEVNYTALLSLGCYDASALAAIKKLAEDVRVDMFYDLKGNQTPENAIS
                     EAKSHSFGGSHQPSRYYFPLSCRDTFTGMNVVYGISCDAFVAKAKGVALVPDVGGWHY
                     APAGISRAIIDRQNIARIPNIGAVDREAFVLARINPVSVAADGSVYIDDSLTTYSKNN
                     YLRFQHVSSLMNAIARDFYEVAQAIKHEPDGITKETLMKAMTELLDRYVAAGALVTPR
                     DKSQGEDPYVVQVVQKDIDLWEVSWSVCPTGTARRIVGKPILMR"
     gene            18302..19036
                     /locus_tag="ACHK0Z_RS09145"
                     /old_locus_tag="pgaptmp_001829"
     CDS             18302..19036
                     /locus_tag="ACHK0Z_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501860.1"
                     /translation="MKNYTADPFMRAIFGSGCFESGDKSTQDNNDNAMLESAGQKGKD
                     TQPKPKDDIETAMMEAVESRAQGDMRSLAASMLAGWVEDGDPEADSFDALAITMAGLA
                     DIDEDTDFTDEQIDAYNDALAALADAAVALGADQDDVTEMIDDEDDSAAERVYDALSE
                     SDTDMMETAIAIYTVAGGDSAMLEAVRKKVVRDGKVTIIRKRPRPRRMTSLQKQALKK
                     ARRKAHTSVANINRKKSMRIRKKRGL"
     gene            19128..19676
                     /locus_tag="ACHK0Z_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             19128..19676
                     /locus_tag="ACHK0Z_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501861.1"
                     /translation="MKVYILSSEDMVVGYIGEGSTAELSSMWQSPFENQSVGGLLGGI
                     SAAAGSLADTLQTATGVTTKTLFNSMLVWEGQQPPEFNLVIDFMATTNAQLEVNAAIT
                     ALLKMESPELNNVAPFGRRPETVTLNIGRNIALTDVVIKSVSYQLDAPRTPEGYFTHN
                     TVTLQCSGNTSINRSNISSVFV"
     gene            19685..20176
                     /locus_tag="ACHK0Z_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     CDS             19685..20176
                     /locus_tag="ACHK0Z_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGU4501862.1"
                     /translation="MSGFANTKADMAFLKSRFNKNLAAGEKLIGSEYWMTIKGYEHLS
                     VLVRTAQLPEMTREDVEDYAPGGMKFNQHGPLRNSGEFQVTCAETIEGAVLAAVKQMV
                     YGKEYLEITLQAAAESNSGNHKGLIRTYSHCKVYSDAVDFSSEDVTAVVKPSLRIVYN
                     WAE"
     gene            20231..20782
                     /locus_tag="ACHK0Z_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     CDS             20231..20782
                     /locus_tag="ACHK0Z_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501863.1"
                     /translation="MTPIELLESVKERFNPLLVREEETLKAFLIKALTTYQDRAGVVK
                     TLKLEKAGGTAIPLPEDYLSLVHVTDNNGLLVYSDELSGFIELELTGSERWPFRMLYL
                     VNLRDRELDEWQVPPAIIGMLEEYLEALINVRNVARQRRASIDGKFDYSDLPDEATLY
                     ARVQEIEEKMSSNRAIIPGATIF"
     gene            20798..21505
                     /locus_tag="ACHK0Z_RS09165"
                     /old_locus_tag="pgaptmp_001833"
     CDS             20798..21505
                     /locus_tag="ACHK0Z_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4501864.1"
                     /translation="MSIFSSVGRTLTTALSFNTKSFTSNLISDILDKAISGGGVSGNY
                     SSDIAYGKNIVAAAMRIRYAQGWQWTVEVDGLNGFDMFVKDITYSSGNIETESKVIGS
                     VEFSKPTYVSAGPVTMTVRDTEDGKIMDWFKERRSRVTNPDGTINLPPEYLMKIRVYR
                     VTQDGGKELEEEMRVFPTQLGEITRSRDQVSEFLSYPITFQKYTSAGSGVSALVNGAA
                     GMATSALKGAVSGVIKF"
     gene            complement(21887..22642)
                     /locus_tag="ACHK0Z_RS09170"
                     /old_locus_tag="pgaptmp_001834"
     CDS             complement(21887..22642)
                     /locus_tag="ACHK0Z_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU4501865.1"
                     /translation="MNRAELTAKAVSLIESATPISRSLAQANEITEAAYHLTRDQKRL
                     LFIVVGRLRYASKDGVLGSGACELTVNEYAEMYNLPSAEASKDIRKAISGLSEKKVTI
                     YNPDESTESEDSYESYPWMIKDAYSPRRGTYIIHLNPYLMPFFTLLDKRFTRLNFTEV
                     SRLTNPYSMRLYESLCQYRKDDGSGFAILGVEWMRERYGLPKSYQRYAEFKRSFLTKA
                     VAEIEKNTKMKIVFSEVTEGGKVTRIKFTYQQS"
     gene            23031..23852
                     /locus_tag="ACHK0Z_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             23031..23852
                     /locus_tag="ACHK0Z_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501866.1"
                     /translation="MNIPKFPLPSRPETEIQFHAPTVKDALKYSDLNPAEDEATTTEY
                     LNSMQDGEINDSANWTVQDRRTALWWIFVNSRPDAVMTYSYECSHCGNTHHADINLSD
                     LAQTVEILTVPPYVKTNVPVNGVPTDWILKPLTGKGAELLERMRASLPDMKSPEYSAG
                     VARMRIAELALCTALDDDPEDFTQAANRRFDIIESMALETEFTPLVARIQLMQKDLRH
                     GLKMSIERGTSRLILPPQHCKNAKEGADVTTTLYVPFLNREFIPSIRSEWMANHY"
     gene            23867..27649
                     /locus_tag="ACHK0Z_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             23867..27649
                     /locus_tag="ACHK0Z_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HGU4501867.1"
                     /translation="MDISPLATLNNYRNGEHSICQKPWKKNTKLKPGNVDRAGKREVI
                     LKENKDRIAIIDAIQEASTNELKALAEVKDAILSGASTITQQDERQGRVSNRLSRRRK
                     DYEQSTSDSVRDVQTNPYKKRLPAKKERAPINQTVDRSIVATNTPESVRSVQSRQTIR
                     KPDGFIPVAANSAPLEPQSPQSLKGPLRDSSGRFVSQKSNEDVARKKELQNARKADAK
                     LQAGFLRKLGSIMGVDGNQSSSEESLTNAAGVGAGGPLWMAARGMYDITKEITGKAES
                     LKEWVEKGKKETSTSKAISPVITYPAVVNSQKATSAKAFNNAVETKSAQAVEEQTKIL
                     QTNDNKIIDGLENVSDEILKLRKSVSSGNKFGLSDLWKNRASRRNKINIGDQTGKNKR
                     NKSKRKGRNLGKKALSAGEKVAAGSAAAGTGVGAAKVVKNKISKPKDVSTISDTSKGI
                     AKETKNTAKAVKSAGVVAEDATIKTGEAIAKKKTESVALKSAAKIGVKSAASTAARAV
                     PIIGSLAMAGYDAIDGYTDTEAQKAAFGLSDDDAVSEQQKTAYATANVLDMGGLVSGA
                     TNLIGKGISALGFERAGEKLQNFDTGDIARGVNGAVDITKSVFGSLKDTFLSTDENTK
                     QVKKAVEDGTKKTVDAIHSLGEQLQGGRNGEDGVGEHGYTSPTEFSAPANNTIAADLN
                     IGGSNAKNRNYRNNNLGNLVFANQEGATLESPNAKGEQRFARFNTPEEGIRALANQVS
                     SYYNGTSAAAGYQKLQTVSSIISKWAPPNENNTNQYIDNVSKYLGVSPNEKIDVSNPE
                     VMTQLVRAIATKEGGNPAVNNEFIKNALGAFNTNTGRWEGQFSDETLARINKIQKENG
                     GQLIARDSQYSVGRKVKYANGKSPAHPVLNAVPTATQQPIEVAQHAQAVKKPQKTGNQ
                     SQPINPENVDVNTGSPSSLLERLIAINDSGVGNLFGGAATSLAGTSLGLIKDFASATS
                     FGSISSLSEKAKGMDQALTEKISSLTGKSFGFQKASQVTDIRDALQKRPKAERDMPSV
                     DLLSGVSSASESEKISVNSRGIPVYDNGYKVIDGEDKGVLGSLFDSSLTGLKRISSAV
                     LPAIGDNVSQLIGGVDGTGIVNDLVYQATGQNSTIARAISPLTRSAGSWLNNGIQQTA
                     DSIRGISNEANNAIFGSASAVQEPFLALPPQLPTVTDLARSGIRQPLTTDTINNDPAM
                     LKALDNICSILNDLLNVNKNNTKGDPDKVVKTSQPQPRPRASTTINDPSLDALLED"
     gene            27655..28029
                     /locus_tag="ACHK0Z_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             27655..28029
                     /locus_tag="ACHK0Z_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501868.1"
                     /translation="MLYEIDARLQTNESGVIIAEGSTAAWMARLDEWLRTPEGSVYGL
                     PSWGNPMEEFKHEPFGSETSHIVEVAIEGRMMKKLRQDLPGLDVQGIRCTSISEDSLL
                     ISFYAKGGSMDIVMQKSSGVGA"
     gene            28026..29456
                     /locus_tag="ACHK0Z_RS09190"
                     /old_locus_tag="pgaptmp_001838"
     CDS             28026..29456
                     /locus_tag="ACHK0Z_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGU4501869.1"
                     /translation="MTITELLDKFNAKLNENTWWSRFVNSQFVQMHAIFGSQLIYIAR
                     TFASRGLTEGLISTATRRSSILAVAEDRSYVGRFVSASYGTTSITNKTDRDITLPAGA
                     ELLANDQTPLAIINSVVIPAGGTVSGVETKQHEAVSVTFDIEKETLFLTLLLSRELTK
                     EVSSLDVYVITDGVEEKWTYNPLFRMSRDKSKHYSLAYKPTEQLGVKFGDGSMGMMPP
                     AGCQVRIDVMASLGDYTLAEGQKLEPAGNIAQYVESLEFKTDSIITGGSGMETTEETR
                     NRAQYYVAYDEQVVWGGDYRQFIQNVVHGTSWLNVWGEALQEKITGFDVRNINKIFFC
                     GHKPGVSQAQLKSEILKALENVPNELNKRFEYVDTNEQPFTINFIGIARKNVLIDDAQ
                     NAIKAALEDNFGRDSSSFSLLLQSDDDSQQCYAQVKVKDIWRVIESLDMFLSYDITIQ
                     NMKDAVYFNDFIYLDVKSSTFSISYP"
     gene            29467..30312
                     /locus_tag="ACHK0Z_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             29467..30312
                     /locus_tag="ACHK0Z_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4501870.1"
                     /translation="MKDNWLKERLTKVKQDSHLWSAFIDALQDVWNEAVEPILTRISN
                     RKSFFTMDSEDMDARIAEYGRFFVITEKDKARRPMLLAQRLDEVHFKGTILPIEQTFW
                     REFGCIPVSWEPLYAPVNIEKHPYGSYFATEIEIPTAQAQFGEFFLTSRGLVVVDQNK
                     LYRSYGEQDKEAAVQKLLSDFETVIAPLLPLHIVFDGVSFRLSAVFPEVAEILNCLST
                     DVSVIEGVYVTESMADMLSRSDTSCQVDNISLNAIPNRTAEKQLHLDVTPLDAWPLDY
                     HLQPV"
     gene            30407..30853
                     /locus_tag="ACHK0Z_RS09200"
                     /old_locus_tag="pgaptmp_001840"
     CDS             30407..30853
                     /locus_tag="ACHK0Z_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4501871.1"
                     /translation="MAENLKARDGNRLYKAQLLSYYYSRRAESAIGKGARFVISKAYW
                     CKSSLVTANGAGGWNIADIPLDFKLSDAQQFAVSDLILSSVDGIITINAAFPQERMPD
                     NTPYDFNTLVLVDAEEQAFGVLCTQQDTLYKGKRYSILMTIEQVEG"
     gene            30856..>32286
                     /locus_tag="ACHK0Z_RS09205"
                     /old_locus_tag="pgaptmp_001841"
     CDS             30856..>32286
                     /locus_tag="ACHK0Z_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4501872.1"
                     /translation="MGADKTNNIMTLSSGVSQPLLADVQYFELYSSSALNRKLKNIVL
                     PGFYCGFEPVPGTGLSVRITSENSEGKGAASVDVNNVQISVQQIEDVTVLVKAGATNI
                     IVLEANFEHGVKTTQVDSASSVSAARIYARTDNTIGQNQIELCRVIVPSGATAVTKEM
                     IVLKYRVNRAVGVEFSNEISSTEERKAATPLAVKTLHDLVDTKAPLDSPHLSGTPTSP
                     TPEPGTNNTQIANAAFVYAAINALINGAPGTMDTLKEIAAAINNDPKFSETINNALAL
                     KAPLASPAFTGTPTAPTASQGTNSTQIANTAFVKAAITALINGAPGTLDTLKEIAAAI
                     NNDPNFSTTINNALALKAPLASPALTGVPTAPTAAQGTNNTQIATTAYVRAAISALVG
                     SSPEALDTLNELAAALGNDPNFATTMTNALAGKQPLDATLTALAGLATGANKLPYFTG
                     KDTVAQTDLTSVGRDILAKTSTLAVIQ"
BASE COUNT         9189 a         7387 c         7568 g         8142 t
ORIGIN      
        1 ttatggatag ttaaaagatt caacaggtga tggtggctga attgggtaat gtttcttctc
       61 gtactcctca atgagaagac tcataacctc aaagaaatca ccttccggag tattcatttc
      121 cggttcgtta tcgaacatcg gttcgaccgc acgcagtgcg gcctcataat cttgttctgt
      181 acggataggt ttaatgttca ttaattacaa aggaaagccc acttgctaag tgggcttttt
      241 gtatgtgttt tactatatca ggctgctttt ttattcaggt tccgctgaga aataaatttt
      301 tcagcagtat tgatacgact taactcttcc tgaacgcgca tatcattttc gacttcccac
      361 agatctactg cggtctgaag attaatccaa aaatctacag aagtatcgaa tgcttttgcc
      421 aggcgatatg ccatatccat cgttagctta cgattattat taacaagagc acttacggtg
      481 ttacgatgaa catgaagtat ttctgctaac tcgttgatct tcagctctaa cggctccagg
      541 tattcgtaca gcaggatatc acctacggtc gtcggttttc tggttgcctg tttcattttt
      601 tgcccttact agcgtgcgta ttaagtggac tctggtcaga acccggtccg taaccgggta
      661 tctgttttag tatttgtgag ggtcgagata caagtcctcg gcctttccgt taacccactt
      721 aaaaattaac ctatattgct tattcaccct tactgatgaa tagccattta gcttccctga
      781 cagttcctca taccgattac ctggtggtga tcttaaatcc ttacaggtag ttgcggcatt
      841 gataatgtcc aacttccgtg acaatgtcat atgaatatca ggaggtatct ttctatgtgg
      901 tgttgaaaat tcaaaaaaat catcaagcca cgcatccctg aaatccttta tgttaatttt
      961 tttggtcatt agtcatttga tcctccgtga tgattaccga atgccataac tatagcgcac
     1021 tgttgcactg tgcgcaagtg cattttatga attccccctc cttcatccaa aatcacaatc
     1081 tcccgaaaat cttcctttcg atacctgaca gatcgacctt tgaggatgct tatgggccgt
     1141 tttgacgaat ggtttgctga cgatgcaaac ctggcaagta aggagtcaga acatgacagc
     1201 acgcagagag aaacgccagc gccgattgaa gcgaatgcag gaggcgcgcc ggaacatggc
     1261 aataccggtt cggattcatt cagcattctg gaagagcaac ccgccgccaa agctgatagc
     1321 catgataatt ttgctgatag caacggtggc cttactccgg atgctggcga atcagatata
     1381 gccatacttc ctccttccct ggctggcaga gaaccaactc cacagctaaa agcacgctat
     1441 aacgggcata aagcctttaa cgaccaaatc cgcgcagact ggatgctgat tattgaatcc
     1501 agcccagacg cgtttcaggc tctcttatat cgaccagatg ttggcacata tggggtagtc
     1561 agcgacgaaa caggagaaga gtcattcact gaactggata acaaccaacg cgaactgact
     1621 taccaggaac ctgaaatcgt ttatgtgctg gataaccctg acgggcgtga ctctttccat
     1681 gcgattgacg cagacggtga gcaggatggc ttaaccgatg atgttctgat tctgcgtatt
     1741 gcagcaaata acgtccccgt cggatcaatt cttgaatgga atgaagagat ggtaaacggc
     1801 gtagcccgcc gctggtggta cgtgcaccgt atttttagct acggcacaca gcatgttggt
     1861 tcgctttact actgcatacc tgccaggaat tttgatacga ctcaaaacgg agtgatcgaa
     1921 tgacttcaaa taaaaccctc ctggcgcgaa ccggtgaatg gcaaacctta cgcacaggaa
     1981 aattccagac cacgggcttt gaaaacgtgg ataacgcgtt tgcgacgcta atcagcaaca
     2041 ttttttctga tatcttactg gtggcccccg cgccggaaga aaaacgcttt gcgtcattcc
     2101 tgaatcgtcc accagcagag cgtgtctata tggcccattt cgacaatgcg atcgagtttc
     2161 ttaaagcagt tcgtcgcgca aatgccgggc aagggagaaa acctgaaaac cagaacatta
     2221 accgggatgc tctccccctg gtcaatatct cacgcactat ggatatcaat tatatcaacg
     2281 atgatcagca gattgaccgc aaaaaagtag ccagtttttg cgaaccagat accggaacgc
     2341 ctttagcaga actggaatac acccaggcca ttctgacgta tgacgttacg ttaatggcaa
     2401 ctgataaagc gaccatgagt ctgatgtgta attcgctggg cgcacggctt cgcctgatga
     2461 caggtacaca atttgaagca gccactcacc ttgttcgtgt cccggtcccg ctgatttgct
     2521 cgatacagga tgccaaagaa gtcggcttta cggatgtttc agcaccaatt ggagaagagc
     2581 gtatttatgc cacacaagcg ccgataagtg tgattgcaga cgtgatcaca gcatgggaat
     2641 tggacgcaaa acgtattatt accgaaacct cgatatctat ggggtgataa gtggcccagg
     2701 agttacaaca atattttcta cagtcagtgc tcattaacga taacaaagtg ccacgagact
     2761 ggatttttac cgcagtatat gtagaaaaaa ccagcctcaa agcccctttg ctaaaactgg
     2821 aaattcatga cgctaccggc accgtaattg atgactggaa agccaaatac ggtgcatcgc
     2881 tggtggctga aatgggcgat ccaaacggta atgcaggcac ttttaaaaca gatttctttg
     2941 ttacgtctgc aatgctggct ggtgatgttg ttaccgttat tgctgtcagt gaagacgtgc
     3001 gcaggtttaa gatcccctcc ccgcgcacaa atttacatac caacaaaaca ccagacgcta
     3061 tattcaaggc atattccggc aagcttaaaa ttaccagcag cgtgctaaaa cgcgcagtta
     3121 catatcatct gaatgccggt gacaaaccgt caaaaatgct ctcggagata gcgcgagaca
     3181 aaggtgcatt atgttgggta tgccgggggg aatttaactt ttacaccctg gctgatctga
     3241 tgaagcaaac gccatcattt acctacgagg ggaataaccc taaagcagaa tacactttgt
     3301 ccaaaatgcg cctactccaa caggaacatg cgacaacagc aaagaatcaa tatcgttttg
     3361 ttgggtattc catgaccgat ggctacgtcg aatatggcga tagctcactc ccagtgcgtt
     3421 atatatccga ctctgatatg gaaactcttc gcaatatgca gctgtctctc gtccccaaaa
     3481 tggatataga agtcgcaggc aatcctgata taaaaccggg gatggtgata gagattatcg
     3541 tataccgcta tgaccaggaa aaccgcattg atgaatcaat gccccgtaag ctgatagtaa
     3601 aaaatgttgc gcactttgag gaccgcgtag gctacacaac acgaatgata ttgggagtgc
     3661 cgaacaaatg aagcgtagag cgcaaattgt tggaactgtg cacccggcag ggcttatgcg
     3721 tgctcaagtc cgtgttttac ctgactggaa tggcgttcct gatgacgatc taccctgggc
     3781 ggaataccag ttacccatcg ggaacgcttt tgtacctaca gtcaaaggtg acctggtctg
     3841 ggtagagttt ccttatttag atgttaacgg tcgaatagac accagacgcc caatgatagt
     3901 tggtgccgct caggatgcac cgggaggaat tccaaacgtt gcaccagaag catctggcaa
     3961 aggcaacggc tggacgccgc cggaagtaga tggagcacct ccccgccccc aaatctcagc
     4021 aacgaaagac ttcgttattc accgcaacaa tattcttgaa gtacgaactg ctggtggcgg
     4081 ctatgaaatt gccaatacgg cagctggctc aaggattggc atgaatgagt ccgggcagat
     4141 atatatcatt ggtccggctg atgtaatcgt gaatgcagga gggagtgtta acgtaaaatc
     4201 agccaacaac atcaatgtta atggggaaaa tattgctgtc accgctaacg gagacatagc
     4261 tttcaaggca ggaggaacct ttcaggccac tgctagcaac ttcgatttca agaaaggata
     4321 gaaaatgccc tcatttatgt tagataatta atatgcattt tctaacacaa atggtgagac
     4381 atgagatcag tcgctttcaa aaatatcttc atctatcgtc tttcacgcga agttagctgg
     4441 gatactgcag aagtaaatgc agcccttagc aagtttgtgt ttaccccatg cggttctcag
     4501 gatatggcta aagctggttg gacccccgtt cttggcgaca acctcaccca tgaataccaa
     4561 ggttttcttc tgatacagca taaacgagag gaaaaaatcc taccctctca agtgctcaaa
     4621 gaagagttac agaaaaaaat cctgacactt gaagaagagc aagacagaaa gctgaagaag
     4681 gctgaaaaag attcgctgaa ggatgaagtg cttcactctc tgcttcctcg tgctttcacg
     4741 agaaaatcag tcgctaaaat cctgatagac cgaagcaatc atctggtttt tgtcgaggcc
     4801 agcagtgcta aaaaagcaga ggatcagcta gctttgttac ggaaatcgct tggcagcttg
     4861 cccgttgtcc ccttctctcc gaaagaccca ttagaactga ccatcactgg ctggattaaa
     4921 aatggcttcc cgaacggttt ccgtgccggt gaggaagcga ccttaaaagc attgcttgat
     4981 gatggcggcg cggtccgctg caaaaaagtg gacctgctat ccgacgagat catgagtcat
     5041 atagaagctg gtaaggttgc aaccacagtt gcggttaact ggcaagaccg tgcatttttc
     5101 agactgaatg acgatatgag tatcaaggct ttgtcgttca gcgacgaatt gtacgaccaa
     5161 aacgacgata ttgatcgaga ggatgttgcg caacgatttg acgctgactt tatcctgttt
     5221 actgctgaat tatccgcgat gttcacttcg ctggtggagg ccgttggtgg tgaagcagaa
     5281 cgttaacaat agacctctga tggtgagtct ttgtgatttc acggggaaaa tggtcgcccc
     5341 ctggctggag catggtatag atgctgttat cgtcgatcca caacacgcag agaccagaga
     5401 tgagctgatg caatcaggcg cagttctgac acgtatcagt gcgattatcg atagtgatga
     5461 agtttatgct tttctccgaa ataatttaca acgtattgtg tttttagctg gttttccgcc
     5521 gtgcacggac cttgctgttt ccggtgcgcg ctggtttgcg cataaggctg acaaagatcc
     5581 gtttttccag ttcaaagcga tgcaggtcgt ctggcagtgt tacgatattg caaagatgat
     5641 tggttgcccg tacatgattg aaaacccggt cagccaaata tcgacattct ggcgtaaacc
     5701 tgatcacatc ttccatcctt accacttcac tgcatattgc aaggaagaca attacaccaa
     5761 aaaaacatgc ctctggagcg ggcaaggctt tgtcatgcct gatgccctaa tggacgaatc
     5821 attaggcaag ccagataaca gaatccatac cgcgccacct ggacctgaaa gggctaattt
     5881 tcgaagtgct actccgcatg gattcgcaaa ggcagttttc gaagcgaata agggggtgct
     5941 ctatgagtaa atctatagca agcatagcca taaaaaaaca ggatacgatg gctgaaatta
     6001 gctatatgcg caccattcgg actccggatg aatatgagcg cccgatcttc aaatgggtag
     6061 gcggtaaatt ctcagagttg cctacagtgc ttgagcatct accacacggc aagcggttaa
     6121 tagaaccatt cgttggtggc ggttccgtat ttacgaatgc aggatttcgc cacaacctgc
     6181 ttaatgatat taacggtgac ctgattaact tctatcagac attgcagcga gagggacatt
     6241 cgctcgtcac gctggcatat agttttttca aaaattacaa caacgctgac gcttacctcg
     6301 aagtgcgcga ggcatttaac agaggaaagt atgaccaact acatcatgct gccgcatttt
     6361 tgtaccttaa tcggcactgt tttaatggcg taacgcggta caaccaaaat ggcgagttca
     6421 acgtggggta tggcaaatac aaagcgccct acttcccacg tgcagagatg gaggcgtttt
     6481 tggctgatga cgtactcaaa aacacttcct ttgtatcagg tgactttgct ggcgtcatcg
     6541 aggcggctgg tgaagacgac gtgatttttt gtgatccgcc gtatgaaccg ctgccggata
     6601 cagaagggtt taccagttat tcaggaaata gcttccgttt cgacgaacag aaacggctgg
     6661 tatctctttt ggtggaagcc caccagcgcg gcgctaaggt agtgataaca aatagtggtg
     6721 cgccaaacat ccgtgaacta tatgaaggaa acgggtttaa agtacatcat atggcagcca
     6781 gacggtcggt ttcctgcaag gcgtcaacac gtatagttgc taacgatata attgcaataa
     6841 tgaagtaaaa aacgccgcag tagcggcgtt tactttttta tgtggcgaaa tacctattaa
     6901 cctaacttcg cccaggtatc acgcagccca acggtgcggt taaataccgg tttttccgcc
     6961 gtagaatggc ggctgtcgag gcagaaataa ccttcacgct caaactggaa tgctttaccc
     7021 gctaccgctt ctttcagcga cggttcagca aagccctgtt tgatcaccag cgattccggg
     7081 ttaatcaccg acaggaaatc atccgcagca cctgggttcg gtacgctgaa cagacggtcg
     7141 tacaggcgaa tttcaaccgg cagcgcatgg gccgcgctta cccagtggat aacgcctttc
     7201 actttacgac catctgccgg atctttgctt aaagtatcgg catcgtaggt acagaagatg
     7261 gtggtgatat tgccttcggc atctttctcc acgcgttcag ccttaatgac gtaagcatta
     7321 cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg
     7381 aaatcggcgc ggtcgatcca aatctcaccg ctaaacggca cctgacggct acccatttcc
     7441 ggtttgtttg gatggttcgg catggtgacc atttcgcctt cgccctggta gttttcgata
     7501 accagtttca ccggatcgat aaccgccatt gcgcgcgggg cattttcgtt gagatcttca
     7561 cggatgcagg attccagcga cgccatctca atggtgttgt cctgcttggt cacgccgatg
     7621 cgtttgcaga actcacgaat agaagccgca gtgtaaccac gacgacgcag accggaaatg
     7681 gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggttaccag ctggttcagc
     7741 ttacgcttgg acatcacggt gtattccaga ttcaggcgcg agaattcgta ctggcgcgga
     7801 tgaacaggaa tggtgatgtt gtccagtacc cagtcgtaca gacgacggtt gtcctggaac
     7861 tccagcgtgc acagagagtg cgtaatgcct tccagcgcat cgctgatgca gtgggtgaag
     7921 tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc aaacttaata
     7981 cggtacagca ccggatcgcg catgacgata aacggcgacg ccatgtcgat tttcgcacgc
     8041 aggcaggctt taccttcttc aaaaccacca gtacgcattt tctcgaacag cgccaggttc
     8101 tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gctgcgtcag ggtgccgcgg
     8161 tattcgcgga tctgttccgg cgtcagttcg tcaacatacg ccagtccttt gttgatcagt
     8221 tcaacagcat aggcatggag tttgtcaaaa taatcggagg agtaacggat gttgccagac
     8281 cagtgaaaac ctaaccattc aacgtcgttt ttaatcgact cgacgtactc gatatcttct
     8341 tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc
     8401 ccgaagttca ggcagataga tttcgcatga ccaatatgca gatagccatt cggttccggc
     8461 gggaaacggg tatgtactgt ggtgtgctta ccactggcca gatcttcatc gatgatctga
     8521 cgaataaagt tagtcgggcg ggcttctgcc tcactcattg tggattcctc aaagcgtaaa
     8581 caacgtataa cagcgtatga tcttataagc cgaataggct gacaaccact agttgattag
     8641 aaattgcttt ctatcgttat ttcaataatg agctttttca tgaagcccac ttttgcgcat
     8701 aagtaaatta atgaaaaatg gtacacaatt ggaaccaaaa aggatgtaaa ggtacaccaa
     8761 attagtggtg tattggtgca cctttggttc aagacttttt aagttctaaa accaacctga
     8821 aagactttat tttgcttctt cagactccgc taacatgagg atagcatggc ggatcatatt
     8881 caatttgctg cgattatgct ttttacttaa cgcatcaagt acttggtact cgtattcatt
     8941 gaaaccaaca cgaatagatt taaaatcacg tggagcgtcc ttatcgagca ctttgttgtc
     9001 ttctggttgc tcacccggca ccaaatcagc cttactggca aaatcctcaa ttcggcgttc
     9061 aagatcgggg tcttttactt cgggggcttt taccgattta cgaggcttaa tcacttaata
     9121 actccttaac caacatctgg atttcacctt tagctttccc gttgtccatc tcaacaacgc
     9181 ccttcccttc agccatgcaa tcgcggtaaa ccttacgatc acgaatgatg gttttcagca
     9241 acttcagttg cggatattca gccaggtact ctttggcctc attggtttca ttcacaaccg
     9301 gattggatgg ggccattgtt aagactgcga acgccttaat gctaggatta ggcatcaggt
     9361 ccaaagcctc ttcaaacact tcaacaaagt gtgccagcgt gtctaaatct ggttgagatg
     9421 gtctgaatgg gaccaacaca atatctgcag cggttatgcc tgtacgcatc tctttgctat
     9481 cgcgcccggc tgtatcaaca actacaaatt cataacgctt atccagatcg agaagtgttt
     9541 ctcgaatatt accaaacttc tggacacaat gaattacagg agaaacctct gccgtgttac
     9601 ggtcagctgc ccagcgggca gctgttcctt gcttgtctgc atctagtaac actacatcct
     9661 tatttgcgcg ggcaagctcc gcacagatgt ttacgcttgt ggttgatttt ccacagccac
     9721 ctttttggct gactaccaat ataatcatat ccgttcctcg gtgtgatttt ggtatcaaaa
     9781 cggttccaga taagagcctt tttgatgcgg gacaggctct ataatggagt cattaatgtt
     9841 tggcaaagta taacatcaat cagaccaaat acaccttata ttgcatccta tttaacacga
     9901 aatcagccca aaattggaac cagatacaaa ataaccccat aacagcacct aagtggtacc
     9961 aaacaaacac taattagaca ccattaaggc ataaaaaagg ctgcaatttg cagccttaaa
    10021 ccagcacatc ttctgtcaat caggtagaga gtcagctaga ttgcgcgccg acgcgctttt
    10081 ggctacaaat tgctccttca ctggctcaat ggaggcaggg cgctcctcaa ccgcagctgg
    10141 tagttccagg aaggatgcca gcatttttcg gatcagctcc gggttattgt cttcggtcag
    10201 cagggaagcc agtaatcctg gaagcctgct atcgacttct cccagcgcaa gccctgccaa
    10261 caatgcagtt cttgccatat cgctatgttt ttttatcttc tcgctcgccg ccttttcatt
    10321 ggcataagcc tctgggcgaa gataaatcat cactttctta cgttcctgag acatggacat
    10381 acctcgttaa ccgattgctt tcaggccatg aacaagagca agttgcgggt catccgccac
    10441 gaagaaacgg tcatcacgaa tattaacttg cacgcgaatt gcatctgcca ccagcggtgc
    10501 gccaccaccg atcaccatga catgcgtata tcctttaaag tcgccaatca catcaagaac
    10561 gcgggtacgc agacggtcaa tgcttgcggc aatggccttt ttcacatcac caatcgctga
    10621 tgggtcattg atattgtcgt tcagataatc ttcatcgtgg cggttaataa tgagctgatc
    10681 cacattgtaa tttgacgttt cggtattggc ccttgcaagt gcttgcctta cttccctggt
    10741 gaccagtgat acaccaagat tcgaatcgcc aaaaacacgg gaaactgccg tcatttgtcc
    10801 agcgaccatt gaaacatcaa gggtagttcc acccaaatcg acgataagaa cagagtgggc
    10861 agggttcaga ttgtcgcaca ggctaattcc cgccgggata gactccgggc gtaccgtaac
    10921 tttcttaata gtgaatacat tgcctttatt cagcgtgacg gggcgcataa gactggcttt
    10981 cttgcgctcg atgttatcga gattgtattg cgcgtcgtcg tcgtaaaact ctgccagagg
    11041 cagcgtgacc acaatttcta cttcctgcgg ctcaataccg cttgtcagca gggcatggtg
    11101 aacagccagg acgttaagcg ggctgtattg ccattccaca ttattcgtcg gcagaacatc
    11161 tggcgatatc aagtcgtatg aatacttttc gtcgtcaatg acatagttaa acggcttgcc
    11221 cgcaccaaat gttgctgacc atccgcgctt aaagctgtta gggctgatgt gagtgcgagt
    11281 ttccccgtcg tgctcccaca gcattttgat attggttgaa ccatcatcga taaaaatgcg
    11341 catcttcttg ctccacttct gagacacatt tgagaatcaa aatgtaattt taaaagagaa
    11401 gcacccaaaa atccacacca aacgagataa aaaagagcgc ttgttgagtt tattttgaga
    11461 atatcttaat cacccactga atgatggtct gatggtgaga tttatttgag aatgaattga
    11521 gaatacaaaa atattccgaa atttaactct ccgtttgaat gacgaaataa caaagtgatg
    11581 acaataggag gataaccagg aggtgaagaa tgagcgcaat agccaccagc gacacccttt
    11641 gttctggtca cggaggattt ccgccgcgac tggtagctga aaccgttccc tggttcaccg
    11701 ttaacggtaa gccagttgtt gtagatggtg ccatgtttcc cagtcacact gacggtaaca
    11761 gtgcacatcc aggggcagct gtatcgacgc gagtatggtt ttcgataggt ggaaagggag
    11821 ttgtctgtgt aggcgatccg ctttcttgtg gttctgttat cgccaacggc gaggatattt
    11881 ttcaggttgc ataatgcttg attcaatagg tgaagttaaa gccttcaaaa ttctgtcaga
    11941 tgccggtatc tctacaccag aatctataac cataagccgg gcagcttctg taaaagcctc
    12001 atcgctcgcc agcgccatac agggaatagt tcacgccgat aaaacctatc ctgattctgt
    12061 ttcggcctgg actactcaat tgcttggctt ttctgaacag ctcaatgagg ccagcaaagc
    12121 gtcttcatta ctggctgatt ctctttcccc atacaccaag ccatcagagc tgttacagat
    12181 gaagattggc tgggaatgct acgcaaaagg taacgagctg acccccatac ctgctttcgc
    12241 ccttgttgag ggaatgggga atgtttccat tccgcaatcc ctaacggacg cattgacggc
    12301 gctaaaactt gatgctctga aaaccgccat gaacgccatt aacgcaaaaa ttgaagctgc
    12361 cggaagtgct ggtggtggtg aaagcgatgg tggacagggt ggtgctggag gcgctcaggc
    12421 tccagtcatt actcaggatg aaattgatgc tttacgggaa gcagtgacag ccgccgaagt
    12481 tttactctcc gaaattaact cagcaagtga gggtgtcgta gcccttacag gtcgcattaa
    12541 aacgtcaacc actcaggcaa ccaaaggttt agagaacgct gtcgccatca cgttaactgg
    12601 ttctctactg gataatgcag taatgagtcc ggcaatcagt ttaatcatgc cacaaggcgt
    12661 tatcgatgcg ctccaaaaga acaccaaaaa ggaaccatag aaacaccact taggcacaca
    12721 ataggatgta aattggtaca tgtttaacac caaaaatgaa cctgaagagg atggttttga
    12781 aaaagataat tttgattgta attgcgcttt tagccatttc tggaatttac cgaacaacga
    12841 cggcagttat cgaacacatc aggtatgtcc gatcgcttga agactcaaaa tcacaactgg
    12901 aaagcacgaa caacacgctt tctaccgcat tacagttaag tgagaacgct cgctcagcca
    12961 tgctgaccga aaacgaaaga attaaagcac tggaaaagga gtaccagcgc aaaacagcag
    13021 aactgaatgc caacctaatc aaacaacgag aggaaagcca gaatgaaatc actcgccttg
    13081 aaaatgcgtt acgccgtgct gggattaacg atgtgcgtgt gcctgatgac gttatcagga
    13141 tgcagcggga acgggcaaaa gccatcaacc agcgtgccag tgaaaattac cgtagaagcc
    13201 accagcaagc cgcaggcaaa tctgattgat tactcccctt gtttagtgcc agaggacacg
    13261 ccagtgctgt taaacaagta tccgacatac gctgaacagt tattcagcac gatcgacgaa
    13321 tgcaaccaac gaaacgcact gaataaccaa agaaacaggg agatttggga agggaagggg
    13381 gatcaaggct cctgaagcca gatgtcctgg ctgtcagctt cttcgaaacc gtgacagacg
    13441 ttaaagtcac tcaagcgata ctgacgctga cggcctctat ttcgctcatg ccaatgcata
    13501 tatccaccag caaatgccag cggacaatca ctatagtcta tgtaaccaat accattcccc
    13561 aaacgtgctt cgcgcgcacc ggctttccag gacagtagaa aagagtcacg atccgccagc
    13621 ataacaacaa aatcgtccgg atctccgata cagtgaagaa tacctgcctt acataactgc
    13681 ttgagcgctt tggactggct caggatgtct tctccgtaat actgaaaaca atcatcaaca
    13741 acagcgggat agcctgccgg gggattcagg ctggttggta tagctgacgc gatcgacagc
    13801 attaagcgat attgtgtttt tgatgggtta acgtggctca ttttgtccct tgtttactca
    13861 ttgtcgccat gcgtttttta actcaggaat atctgccatt tcaattaact tgcgaagtcc
    13921 agcactgact ttcccattac caaccatagc aagccaatct cttactgctt tcggcatgta
    13981 gagcgttgct gttaccggct cttcaaccct ggcaggacat ccgcatacag gccatttaat
    14041 tgattccata acttcggagg ctggggtgag ggcggggaca ttgagtatat ctctaccaga
    14101 aaactggata acgatccgtt cagctagcga gagtatctca ctttcggcaa gaattgatgc
    14161 caactgcgcc tcaacaagaa agcgacttgt tgtgttcaga gagtggctct tagcgccatt
    14221 tttatacgaa acaaagattc gatacgccat aactctaatg atttgtgtta gaaaactaaa
    14281 attaaatcac ataacaacat gcgtgttaat atattttatt ttactaagcc tattgactaa
    14341 atcacaaagt atgtattgtt agcacgtcaa aagaaccttc acgccgcgcc cgaatttagg
    14401 gcttaaccgt gaataaaaac attcagccag ctcgtgatcg cgagcgcgag attatgaatc
    14461 gggcggcaag cgtcatggct atgaccgtcg atccgaccac cgatgcagca ggcaacatga
    14521 ttgctgacca ggcagtaatg atggaaaacc tggataaagc aatccagaaa gtgcctatgt
    14581 ttgaaggcgt aaatccagag gtcgctcgtc agatcactgg tgggtgggct atgtctctgc
    14641 atgagtataa acgtcaacat ggtcattacc cggcaagtga catcctggcg aacgcacaca
    14701 tggctcttga acgcctgatg actgaatgtg ccagtgatac ccatgaaggc acgggtaagg
    14761 caatgtttga gtctgtcgcg caatcaatgc gtagctctga cggcgtaatg aaagttgctc
    14821 aatatgctgc tctaattctg cctgcctctc tgggcgctgc taccagtgat gcctgtactt
    14881 ttgttccttg tgatcgtgac gagtcaaata tttatgaact ggtcaacgta gcaggcacaa
    14941 aattcggcac cttcaaacaa ggtgacgaac tgaatatgca gtcagctggt gtttattctc
    15001 aaatgaagcg cctgtatacc ctttccacta aaggggatgg cagcaccaaa accttccagt
    15061 tcgacatcca aaattttgag ggacaatcct gcccgcttcg cgctggttat aacaaactgc
    15121 tgatcaaccg caaaccgtcc aaagttgacg acggcgatgg caacctttat ttcaatgcta
    15181 aagacagcaa aggtaatgct ttctcagcga ctgccaaagt tgcctatgac actggtgtca
    15241 ttgacattac atttacccag gctccgccag aaggtactga aattgctgtt caggtcgaaa
    15301 ttaacatcga gcgcaatcca agcctgattc cggtgatcaa ccaggctatg cgtaagtacg
    15361 aagttcgtcc gtcccagtac gtaattgctt ccgaacacac ggtaatgtcc gcatccgatt
    15421 taagccgtga gcacggcctt gaattagcgg cacttcagtt ctccgcgatg cgtaactgga
    15481 tctcccatga aaccgacatc atgcgtctgc gcaccctggt attccatacc gtttatggtc
    15541 gtgaatttga tgtggctctg ccggaagctc aaaactatga gtcctgggtc ggcctcctgc
    15601 gtcacgtagt aaacgctctg tctcaagaaa tggcaaatcg taccctgaca acgggtattc
    15661 gtggcggctt tgctggtggt gacgccgcga acttcctgcg ctctctgcct ccacagcact
    15721 tccagatcgc accaggttac gttcagtcgc cgtatgtaca gtacatcggc accctgtttg
    15781 gcacgattcg tatttacgaa gtgccgcaac ctgtctgtga gcaattccag gcacagggat
    15841 atgacttcgg gctggatgac atcttcttct atgggcgtgg cgaaggcatc ggtaaagccg
    15901 gtctgattgc tggtgatgca gtcccagcaa tcccttacgt gcacgaaacc aatccgtctc
    15961 tcgttaaccg taccactctc tggggcagtg ccatcaacga agtacatcct cgcaacggtg
    16021 aaaactactt cacccgcctg cgtctgactc gtgccaaaga aggtgctatc gacatgctga
    16081 caggcaaggt taacgaaaaa aagtgatggt ggcgaacgtg tcggtatcac caacgtcact
    16141 tgacgtagat gaaggtgaaa ctatcgccgc gaacacttca aaggcacaga aaaaaacaaa
    16201 gcagaagtaa ggcaaaaaca cgcccctgaa taaggggcgt tcaggagaaa agaatggcaa
    16261 acattcaagt aactatcacg ccatctgacg caacagacaa atctttcact gttgagtcag
    16321 atcaccctga aattgtccag gttgatggca ctacatgtac cgctcttaag gccgggcagg
    16381 cagtgctgac aatcaaaaca aatgacggca ataaaaccgc acagtgcaca gtcactgtta
    16441 gggaggcacc taaaagcgta agcgccgtca gtgttgaacc aactacgaaa gaagtaaccg
    16501 taggcgacaa cttcacggta agcgaatcct gatcagaggc tgcccattaa ccgtgggtag
    16561 ccttgcctcg ttctctcgct ttagaggata agaaccagca tgaataagat tagtttttcg
    16621 gtagggcagg cagctggcgt tgctgtaatg tcagtaaatg ctgacgctac cctcaccaat
    16681 accagcggtg gcgcatccgt ctttgctggg cttgtaatat cgcgtcgtgg cgcaccgggt
    16741 aaggtactga aagttgacga tacgacttat caaagcgtat taggttcacc gattcaccct
    16801 cgccagggcg cggcatttga accatatcgc cacgtggagc gtgcagtaaa aggcggctct
    16861 ggatatgtcg ttcgagtttg cgctaaagac atgaaggtgc cgggtatttc tgtttccgtt
    16921 gtgggcaaag caaaagcggc aaaggcgagc aaagaacttt ccgttgaacc tactgaaacc
    16981 acagtaaatc caggtgacac attaagcgtt ggtgttgctt caactgcaac gactcaatca
    17041 gtgtccttca cacccaaaga gacgccccag attaaaggtg aagagaaggc attattcttc
    17101 atcaaagatg gtgatgcatc tcaaaatcgc acgttatcac taactcgcga tgatgaagaa
    17161 agcgaactct ttacgctgac gctgaaagaa aaacaaaccg atgggtccat tgaggtgctg
    17221 gaaagccacc aagtgtcttt caatccagaa ggcactaacg acatgggcca accagcatgg
    17281 attccgacac tgcttgaaag ccagtccact cgcattggtg ccgtactggc agataatgca
    17341 gaagcatccg ctgctcaact catttttgag gatgtagctt ttgaaggtgg tactgatgga
    17401 gatctgtccg aaattgacac tgaagactat ctggaagcat taaaggttct cgaagcttca
    17461 gaggttaatt acaccgcatt gctgtcactg ggttgttatg acgcatccgc cttagccgca
    17521 atcaaaaagc tggctgaaga tgttcgcgta gacatgttct atgacctgaa gggcaaccag
    17581 acacctgaaa acgctatttc agaagcgaaa agccatagtt ttggcggctc acatcaacca
    17641 agccgttact acttcccgct ctcctgccga gatactttca ctggaatgaa tgtcgtctat
    17701 ggcattagct gcgacgcatt cgtagcgaaa gcaaaaggag tggcgctggt accggatgtt
    17761 ggtggttggc attacgcacc ggcaggtata tcacgcgcga ttattgatcg acagaacatt
    17821 gcccgaattc caaatatcgg tgcagttgat cgtgaagcgt tcgttcttgc gcgcattaat
    17881 ccagtttcag ttgctgctga cgggtccgtt tacattgacg actctttaac aacttacagc
    17941 aaaaacaact atctgcgatt ccagcatgtg tcttccttga tgaacgccat cgccagagat
    18001 ttctatgaag tagctcaggc gattaagcac gaaccggatg gcattaccaa agaaacgcta
    18061 atgaaagcaa tgactgaatt gcttgaccgt tacgttgcag ccggtgcgct ggttactcca
    18121 cgtgacaagt cccagggtga agatccatat gttgttcagg ttgtccaaaa ggacatcgat
    18181 ctgtgggaag tgtcctggtc tgtttgtccg accggtacag ctcgccgaat cgtcggtaag
    18241 ccaattctga tgcgctgatt tataaccccc gctaaatgat gcgggggaga gaggtttgaa
    18301 tatgaaaaat tatactgctg atccttttat gcgtgcgatt ttcggttctg gctgctttga
    18361 aagcggtgat aaatccacgc aggacaataa tgataacgcc atgctggaaa gcgcaggaca
    18421 gaaaggaaag gacactcaac caaagcccaa agacgacatt gaaaccgcga tgatggaagc
    18481 ggttgaatcc cgtgcacaag gtgatatgcg cagcctggca gcttccatgc ttgctggttg
    18541 ggttgaagat ggtgatccgg aggctgactc atttgatgca ttagcaatca ctatggctgg
    18601 cctggctgat atcgatgaag acaccgattt caccgatgag caaatcgatg cttataacga
    18661 tgctctcgca gctcttgctg atgcagctgt tgcgcttggc gctgatcagg acgatgtaac
    18721 cgaaatgatc gacgatgaag atgactcagc tgccgaacgt gtttatgacg ctctttccga
    18781 aagcgacacc gacatgatgg aaacagctat tgccatttac accgttgctg gtggtgatag
    18841 cgcaatgctg gaagcggtac gtaaaaaagt ggttcgtgat ggcaaggtta ccatcatccg
    18901 taaacgcccg cgtcctcgcc gcatgacgtc attgcaaaaa caggcgctga aaaaagcacg
    18961 ccgcaaagca catacatccg tagcgaatat caaccgcaaa aaatcaatgc gcattcgcaa
    19021 aaaacgcggt ttgtaagtga cataggccgc ctgcaaaggt ggcctctacc tgggagaaat
    19081 agcgtttgat ttgcggcgcg ataatgcctg acggaataag tccgttaatg aaggtttata
    19141 tcctctcttc agaggatatg gtcgtcggtt atatcggtga aggatctacc gcagaactgt
    19201 catcaatgtg gcaatcacca tttgaaaacc agtcagtagg aggtttgctt ggcggtatta
    19261 gcgctgcggc tggctctctt gcagatacgc ttcagaccgc aaccggcgta acaacaaaaa
    19321 ccctcttcaa ctcaatgctt gtttgggaag gtcagcaacc gcctgaattt aatctggtaa
    19381 ttgatttcat ggcgacaacg aacgcgcaac ttgaagtgaa cgcagccatt acagcattgc
    19441 tgaagatgga atcgccggaa ctgaacaatg tagcgccatt cggtcgtcgc ccggagacag
    19501 taacgttaaa cataggtagg aacatagccc ttaccgacgt tgttatcaaa agcgtgagtt
    19561 accaactgga cgccccgcgc acaccggaag gctatttcac tcataacacg gtaaccctgc
    19621 aatgcagcgg taacacatcg atcaaccgta gcaatatctc atctgttttt gtgtaggagt
    19681 atttatgtcc ggttttgcaa atacaaaagc cgatatggcc tttctaaaat cccggtttaa
    19741 taaaaacctg gctgcaggcg aaaaacttat tggttctgaa tattggatga ccattaaggg
    19801 ttacgaacat ctgtctgtac tagttcgtac agctcagtta ccagaaatga cacgtgaaga
    19861 tgtggaggat tacgcccctg gtggcatgaa attcaaccag cacggaccat tacgcaactc
    19921 tggtgaattt caggtcacat gcgcggaaac tatcgaaggc gcagttctcg ccgctgtaaa
    19981 acagatggta tacggaaaag aatatctgga aattacctta caggcggcgg ctgaatcgaa
    20041 tagcggtaac cataaaggtc tgatccgcac atattctcat tgcaaagtgt attccgacgc
    20101 cgtggacttc tcatccgaag acgtaacggc ggtcgttaaa ccttccctgc gcattgttta
    20161 caactgggcg gaataatccc tatcccgccc ttgtggcggg aacatccttg ttattgagtt
    20221 gtaggcaaca atgacaccaa ttgaattatt agaaagcgtt aaagagcgat ttaatccgct
    20281 gcttgtgcgt gaagaagaaa cgttgaaagc attcctgatc aaagctctga ccacgtatca
    20341 ggacagggca ggggtagtga aaacgttaaa acttgaaaaa gctggtggta ctgcaatccc
    20401 gttgccagaa gattatctct ctctggttca tgttaccgac aacaacggct tgttggtcta
    20461 ttcggatgaa ttatccggat ttattgaatt agaactaaca ggttcagaac gctggccttt
    20521 tcgaatgctg tatctggtta atctacgcga cagagagctg gatgaatggc aagtgcctcc
    20581 tgcaatcatt ggaatgctgg aggaatatct ggaggcgctt atcaatgtaa gaaatgtcgc
    20641 ccggcagcgt agagcgtcca tcgacgggaa atttgactac tccgatctgc ccgatgaagc
    20701 cacgctatat gcccgcgtgc aggagattga ggaaaaaatg tcctcaaacc gggccattat
    20761 tccgggggct accatttttt aacgctggag gcgcagagtg agtattttca gcagcgttgg
    20821 tagaacgttg accaccgcat tgtcatttaa cacgaaatca ttcaccagca acctcattag
    20881 cgacattctt gataaagcca tatcaggcgg cggggtaagc gggaattaca gtagcgatat
    20941 tgcctacggg aaaaatattg ttgctgccgc tatgcgtatc cgttacgccc aggggtggca
    21001 gtggaccgtt gaagtggatg gcctgaacgg cttcgatatg ttcgtgaagg atatcaccta
    21061 cagcagtgga aacattgaga cagaaagcaa ggtgatcggc agcgtagaat tcagcaagcc
    21121 aacctatgtt agcgccggac cggtcactat gaccgtgagg gataccgaag atggcaagat
    21181 catggactgg ttcaaagagc gtcgttctcg tgtaacaaat cctgatggaa caataaacct
    21241 tcctcctgaa tatctgatga aaatccgtgt ctaccgggtg actcaggacg gcggaaaaga
    21301 actggaagaa gaaatgcgcg tatttcctac gcaacttggg gaaatcacac gctcacgtga
    21361 tcaggtgtca gagttcttgt catacccaat cactttccag aaatacacat ccgcaggctc
    21421 aggtgtttct gctttggtca acggtgccgc tggcatggct acaagtgcgt taaagggggc
    21481 tgtgagtgga gtgatcaaat tctgaatagc tttaattatg tgtgctggag ggttgctcat
    21541 ttcccgtcag cacccacgcc ctcaacgata gcgggtaggg aaggtagcgc aattttgagg
    21601 gcatttttta catcatcaat tcatctgtaa tgaaggcatt ctttacacat ttcaataata
    21661 tcaatcagtt aacacagtga atgataataa aaaagcgcaa taagaatgca cgatacgcac
    21721 gatattggtt gagggcgatt tttacatagt ccttacatat caatacgtta tataaaaaca
    21781 caacttgcta ataacaagat acatgcaatt aagatgtaaa aattgccctc aattgccgat
    21841 cgttaagggc aaattctaca ccttgagagg ggtgtaaaaa ttgcccttaa gactgctgat
    21901 aggtaaattt tatcctggta actttgccgc cttccgtcac ctctgagaag actattttca
    21961 tttttgtatt tttttcaatc tctgctacag ccttcgttaa aaaacttctc ttaaattcgg
    22021 cataccgctg atagcttttt ggtaacccat aacgctcccg catccattcg acgccaagta
    22081 tggcaaagcc gcttccatca tccttcctgt actggcataa ggactcataa agccgcatag
    22141 aataaggatt tgtaaggcga gatacttcgg taaaattcag ccttgtgaat ctcttatcaa
    22201 gcagagtaaa aaacggcatg agatatggat taagatgaat aatgtaagtc ccgcgccgtg
    22261 gtgaataggc atccttaatc atccaaggat aagactcata actgtcttct gattcggtcg
    22321 attcatcagg gttatatatc gtaactttct tctcgctaag ccctgaaatg gccttacgaa
    22381 tatccttgct ggcttcagca gagggcaaat tatacatctc tgcatattcg ttgaccgtca
    22441 actcacaggc acctgaacca agaacgccat ccttagaagc atagcgaagt cttcccacca
    22501 caataaacaa cagtcgcttt tggtcgcgag tcaggtgata agcagcctcc gtgatctcat
    22561 tggcttgtgc caaactacga ctaataggtg tcgctgactc aatcaggctg acggcttttg
    22621 ctgttaattc cgctctgttc ataaccctgt gtgatccttc aaatggtgag ggcaattttt
    22681 acatcacttt gagggcaatt actacacctt ttgagggcaa atcttacacc ttttgagggc
    22741 aaatcttaca ccgatcgtat ttaattgagg gcaaatctta caccagcctc ctctacagcc
    22801 cgcgccacta ctggctccag cgacgcctaa aaaggatcta aaaaggatta aaaatggaaa
    22861 aaggtttata aaaatactct gtggatatgt gagtagcgtc acttaaggtg taaaaatcac
    22921 cctcaataat cgcttctcga ccagcaaaga tcttcattca atccccatcg caaatcacac
    22981 actccctatc ctctggaaaa tgaacagata cctgcttttc ggaggaataa ttgaacattc
    23041 caaaattccc attgccttcc cgtcctgaga cagaaattca gttccacgcc cctaccgtga
    23101 aggatgcgct gaaatattct gacctgaacc cggcagaaga tgaggcaact actacagagt
    23161 accttaactc tatgcaggat ggtgaaatta acgacagtgc taactggaca gttcaggatc
    23221 gcagaactgc tctttggtgg atatttgtta attcgcgccc cgatgcagta atgacctact
    23281 cctatgagtg cagtcactgc ggtaatacgc atcatgcaga tattaacctg agcgacctgg
    23341 cccaaacagt agaaatactc actgtacctc cttacgtgaa aaccaacgta ccagtaaatg
    23401 gagtaccaac tgactggata cttaaaccat taaccgggaa aggcgcggaa ctccttgaac
    23461 gaatgcgagc gtcacttcct gatatgaaaa gccccgaata cagtgcaggc gtggcccgta
    23521 tgcgcattgc agaactcgct ttatgcacgg cactggacga cgatccggaa gacttcactc
    23581 aagcagctaa ccgacgcttc gacatcattg aaagcatggc gcttgaaacc gagtttacac
    23641 cgcttgtggc tcgcatacag cttatgcaaa aagacctacg ccacggcctg aaaatgtcta
    23701 ttgagagagg cacaagccga ctgatcctgc ctccgcaaca ttgcaaaaac gctaaggagg
    23761 gtgcagatgt gacaaccaca ctgtacgtcc cctttctcaa tagagagttt atcccatcaa
    23821 ttagatctga atggatggct aaccattatt aacaacctga ctctatatgg atatcagccc
    23881 gttagcgacg ttgaacaatt accgcaatgg cgagcactcc atatgtcaaa agccctggaa
    23941 gaaaaataca aagctcaagc cgggaaacgt tgatcgcgct ggtaaaagag aggttatttt
    24001 gaaagagaat aaagaccgta tcgcaataat tgacgctatt caggaagcga gcacaaacga
    24061 acttaaagct cttgctgagg taaaagacgc cattctttct ggggcaagca ccataactca
    24121 acaggacgaa aggcaaggca gagttagcaa cagactatca cgcaggcgta aagactacga
    24181 gcaaagtaca tctgatagcg ttagagatgt ccaaactaac ccatataaaa aacgtctccc
    24241 tgctaaaaag gaaagggcac cgataaacca aaccgtggac cgctctatcg ttgcaacgaa
    24301 cacaccagaa agtgttcgct ccgtgcaatc acgtcagacc atccgaaaac cggacggttt
    24361 tataccggta gctgctaatt cagcccctct tgaaccacaa tccccccaaa gtctaaaagg
    24421 cccgctgcgg gatagtagtg gacgttttgt atcccaaaaa agcaatgagg atgtagccag
    24481 aaaaaaagaa ttgcagaacg cacgtaaagc cgacgcaaaa ttacaagctg gctttctcag
    24541 aaagctaggt tccattatgg gggtagatgg caatcagtct tccagtgaag agtctttaac
    24601 aaatgcagct ggtgtcggtg ctggtgggcc gttatggatg gcggctcgtg gcatgtacga
    24661 cataactaaa gagatcacag gtaaagcaga atctcttaaa gaatgggtag aaaaggggaa
    24721 aaaagaaaca tcaacctcga aagcgatatc accagtaatt acttatccgg cggtggtgaa
    24781 ttctcaaaaa gcaacatctg caaaagcgtt caataacgct gttgagacaa aatctgcaca
    24841 agcagtagaa gagcaaacca aaatccttca gaccaatgac aacaaaataa ttgatggtct
    24901 ggaaaatgtt tctgacgaga tacttaagct tcggaaatca gtgtcttctg gaaataagtt
    24961 cggattaagt gatctctgga aaaatcgagc aagcagaaga aataaaatca atattggtga
    25021 tcagacggga aaaaataaac ggaacaaatc gaaaaggaaa ggccgcaatc ttggaaaaaa
    25081 agcactctcc gctggcgaaa aagttgctgc cggtagcgct gcggctggca ctggtgtcgg
    25141 agcagcaaaa gtagttaaaa ataagatcag caaacccaaa gatgtaagca ctatcagcga
    25201 tacatcaaag gggattgcaa aggaaacaaa aaatactgct aaggccgtga agtcagctgg
    25261 tgttgtggct gaagatgcca ccattaaaac cggcgaagcc attgcaaaga aaaaaactga
    25321 atctgtagcc ctcaaaagtg ctgcaaaaat aggtgttaaa tcagcagcat ccacagcggc
    25381 aagagcggtc cctatcatcg gttcgctggc aatggctgga tatgacgcca tagatggtta
    25441 cacagataca gaggcgcaaa aggcagcctt tggtttaagt gacgatgatg ctgtatccga
    25501 acagcaaaaa acggcttacg caacagctaa tgtgctcgat atggggggct tggtgtcggg
    25561 cgcaacgaac ttgataggga aaggaatttc tgcgctagga tttgaaagag caggtgaaaa
    25621 acttcagaat tttgataccg gagatattgc tcgtggtgtt aacggcgcgg tagatatcac
    25681 caaatctgtt tttggtagcc ttaaggatac atttttatcc actgacgaaa acacgaaaca
    25741 agtaaaaaaa gctgttgaag acggcaccaa aaaaactgtc gatgcgattc attctctagg
    25801 tgaacaatta caaggtgggc gtaatggcga ggatggagtt ggtgagcacg ggtatacttc
    25861 tccaactgaa tttagtgcac ctgccaacaa caccattgca gcggatctga atattggcgg
    25921 cagcaacgct aaaaaccgca attaccgaaa taataatctc ggcaatcttg tttttgccaa
    25981 ccaggaaggg gcgacgttgg aatcgccaaa tgcaaaaggt gaacaacgtt ttgcgcgatt
    26041 taacacgcct gaagaaggga tcagggcact ggcaaaccag gtttccagct attacaacgg
    26101 caccagtgct gcagcgggat atcagaagtt acagacggta tccagcatca tttcaaaatg
    26161 ggcaccgcca aatgagaaca atactaacca atatattgat aacgtcagta aatatcttgg
    26221 tgtatcgcct aatgaaaaaa tagatgtcag caacccagag gttatgacgc aattagttcg
    26281 tgcaattgcg acgaaagaag gcggaaatcc ggcagttaat aatgagttca ttaagaatgc
    26341 acttggagca tttaacacaa acactggtcg atgggaggga caattctctg acgaaacatt
    26401 ggcgcggatt aataaaattc agaaagaaaa tggtggtcag ctaattgccc gcgattcgca
    26461 atatagtgtt ggtcgcaagg taaaatatgc taatggaaaa tctccagctc atccagtttt
    26521 gaatgccgtt ccgacagcaa cacaacagcc aattgaggtt gcacaacatg cccaggcagt
    26581 caaaaagcca caaaaaacag gcaatcaatc gcaaccgatt aatccagaga atgttgatgt
    26641 taatactggc tcgccatcaa gtctgcttga gagattaatc gctataaatg acagcggggt
    26701 aggtaattta tttggaggtg cagctaccag ccttgctggt acaagccttg gcctgattaa
    26761 ggattttgcg tcggcgactt cgtttggttc tatatcttcg ctttccgaaa aagcgaaagg
    26821 aatggatcag gctcttactg aaaagatatc cagcttaacc ggtaaatctt ttggatttca
    26881 aaaagcaagc caggtaactg atataagaga cgctttacaa aaaagaccaa aagctgaacg
    26941 cgatatgcct tcagtggatc ttctctccgg tgtttcttcg gcaagtgaga gtgaaaaaat
    27001 ctctgtaaac tcaaggggga ttccagttta tgacaatgga tacaaggtaa ttgatggtga
    27061 agataagggg gtgcttggct ctttgtttga ctcgtcatta acaggtctga aacgtattag
    27121 ctctgctgtg ttacctgcaa ttggcgataa cgtttcacaa cttattggtg gggtagatgg
    27181 cacaggcatt gttaatgatt tggtttatca ggctactggg cagaattcaa caattgccag
    27241 ggctattagc ccgcttacca ggagcgccgg tagttggctg aataatggta ttcaacaaac
    27301 cgcagatagc attagaggta tatcaaacga agccaataat gccatatttg gctctgcgtc
    27361 agccgtgcaa gaaccttttc ttgctttgcc accacagctt cctactgtaa cagatctcgc
    27421 acgaagcgga ataagacaac cattaactac tgacactata aataacgatc ctgctatgtt
    27481 gaaggcgctg gataacatct gctctatcct gaacgatctt ctgaatgtga acaagaacaa
    27541 tacaaaaggc gatccggata aggttgtcaa aacatcgcag ccgcaaccgc gaccacgtgc
    27601 tagcacaact attaatgacc cgtcgctgga tgctctgctt gaggattaat acagatgctt
    27661 tatgaaatag acgcaagact tcaaacgaat gagagcggtg tcattattgc agaaggaagt
    27721 accgcagcat ggatggcccg acttgacgag tggctacgaa cccctgaagg gagtgtttat
    27781 ggtttaccct cttgggggaa tccaatggaa gaatttaagc atgaaccatt cggctctgaa
    27841 acttcgcaca tagttgaagt ggctattgaa gggagaatga tgaaaaaact acgacaagac
    27901 ttgccaggtt tggatgtgca ggggattcgt tgcacatcaa tttctgaaga ttctttatta
    27961 attagttttt atgcgaaagg tgggagcatg gatattgtta tgcagaaatc aagtggggtg
    28021 ggcgcgtgac gattacagaa ttactggaca aatttaatgc caagttaaat gaaaacacat
    28081 ggtggtcacg atttgtaaat agtcagtttg tgcagatgca cgctatattc ggatcgcagc
    28141 ttatttatat tgcccgtaca tttgcaagcc gcggcctcac tgagggactt atttcaacgg
    28201 ctacgcgccg ctcaagtatt ttagcggttg ctgaagaccg tagctacgta gggcgatttg
    28261 ttagtgcttc atacggaaca acgtctataa ccaataaaac tgatcgggat attacgttac
    28321 cagccggggc tgaattgctt gccaatgacc aaacaccttt ggcaattatt aacagtgttg
    28381 taattcctgc tggaggaact gtttctggcg tagaaactaa acagcatgaa gctgttagcg
    28441 ttacatttga tattgagaag gaaactttat ttctgacatt gttgctttcc agagagttaa
    28501 caaaagaagt ctctagcctt gatgtttatg ttattacaga tggcgtagaa gaaaaatgga
    28561 catataaccc attatttaga atgtccagag acaagagtaa gcactattca ttggcatata
    28621 aacctacaga acaacttgga gtcaagtttg gtgatggttc tatgggtatg atgcctccag
    28681 caggctgtca ggttcgaatc gatgttatgg ctagccttgg cgactatact ttggctgaag
    28741 gacaaaagtt agaaccggct ggaaatatcg ctcaatatgt ggagtcacta gagtttaaaa
    28801 ctgattcgat cattaccggt ggtagtggta tggaaactac agaagaaact cgaaatcgtg
    28861 ctcagtatta tgttgcatac gatgaacaag tggtatgggg cggtgattat cgccaattca
    28921 ttcagaatgt tgttcatgga acttcatggt tgaacgtttg gggcgaagcg ttacaagaga
    28981 aaataactgg gtttgacgtt cgaaacatca acaaaatttt cttttgcgga cataagccgg
    29041 gtgtaagcca ggctcagcta aaatcagaaa tactgaaagc tctagagaat gttccaaatg
    29101 agttgaacaa gcggttcgag tatgtagata caaatgaaca gccatttact ataaatttta
    29161 taggtattgc acgtaaaaat gttctgatag acgatgctca aaatgctatt aaagcagcgt
    29221 tggaagataa ttttggccgt gattcgtcat cattcagttt attactacag agtgatgatg
    29281 attcccagca atgttatgca caggtaaaag ttaaagatat ttggcgagta atagagtctc
    29341 tggatatgtt tctgtcttac gacataacaa tacaaaatat gaaagatgct gtttacttta
    29401 atgacttcat ttatcttgac gtgaaatctt caacgttcag catttcttac ccgtaatgag
    29461 gttagcatga aagataattg gcttaaggaa cgattaacta aagtaaaaca ggattcccat
    29521 ctctggagtg cctttataga cgctttgcaa gatgtctgga atgaggcagt agaaccaata
    29581 ttaacaagaa taagtaacag aaaatccttc ttcactatgg acagtgagga tatggatgcc
    29641 cgcattgctg aatacgggcg ttttttcgtt atcactgaaa aagataaggc ccgtaggcca
    29701 atgctgctgg cgcaacgttt agatgaagtg cactttaaag gcactatctt gcctattgag
    29761 cagacattct ggcgtgaatt cggttgtatt cctgttagtt gggaaccgct atatgcacct
    29821 gtaaatatag aaaaacaccc ctatggttca tattttgcga ccgaaataga aatacctacg
    29881 gcacaggctc aattcggtga gttcttcctg acatcaaggg ggctggtcgt tgttgatcag
    29941 aacaagctat atcgctcata tggagagcag gataaagaag ccgctgtgca gaaattgtta
    30001 tctgacttcg aaacagtaat agcccctttg ttgccattgc atattgtttt tgatggcgtc
    30061 tcgtttcggc ttagtgctgt attccctgaa gtggcagaaa tactaaattg cttgtctacg
    30121 gatgtttcgg taattgaagg tgtttatgtt actgagagta tggctgatat gctatcccgt
    30181 agtgatacct cctgccaggt agacaatatt tctcttaacg ccataccgaa ccgaacggca
    30241 gaaaagcaat tacatcttga tgtaacccca ctcgatgcgt ggcctttgga ttaccacctt
    30301 caacctgttt aataattctt cccccgcatt tgggggaaga acaacggaaa tcacaaactg
    30361 agcatactct cctttggttc attgcttatg aaggggctat gttcgtatgg ctgaaaattt
    30421 aaaggcaaga gatggtaacc gcctgtataa ggcgcaattg ctttcctact attattcacg
    30481 ccgcgctgag tcggcgatcg gcaaaggtgc gcgttttgtt atctctaagg cgtattggtg
    30541 caaatctagt ctggtaactg ctaatggtgc aggaggctgg aatatagctg acattccact
    30601 cgattttaaa ttgagtgatg ctcaacagtt tgcagtgtcc gatctgatat tgtccagtgt
    30661 ggatgggatt atcactatca atgcagcctt ccctcaagaa agaatgccgg ataacacgcc
    30721 ttacgacttt aacacgcttg tgcttgttga tgctgaggaa caggcttttg gtgtgctttg
    30781 cacccagcaa gataccctct ataaaggtaa acgttacagt attctcatga caatcgagca
    30841 agttgagggt tgattatggg ggctgataaa acgaacaaca taatgacact atcctctggt
    30901 gtctcacagc cgttgcttgc tgatgttcaa tatttcgaac tctatagtag ttcggctctt
    30961 aacagaaaac ttaaaaatat tgttttgcct ggcttttact gtggatttga accagttccc
    31021 ggcacagggt tgagcgtccg tataacttct gaaaactcag aaggtaaagg ggctgcttca
    31081 gtagatgtaa ataatgttca gatatccgtt cagcaaatag aagatgtgac tgtcttggta
    31141 aaggctgggg ctaccaacat tattgtgctg gaagccaatt ttgaacatgg tgtaaaaacg
    31201 acacaggtag atagcgcatc ttctgtcagc gctgcaagaa tttacgcgcg tacggacaat
    31261 actattgggc agaatcaaat tgaattatgt cgagttatcg tgcctagcgg cgcaacggct
    31321 gtgactaaag aaatgattgt gcttaaatac cgagttaacc gtgctgttgg tgtcgaattc
    31381 tctaatgaaa taagcagtac agaagaaaga aaagcggcta cacctctggc tgtcaaaact
    31441 ctacatgatt tggttgatac aaaagcaccg ctcgatagtc cgcatctgtc aggtacgccg
    31501 acttcgccaa cacctgaacc cggtacaaac aacacacaga tcgcaaatgc ggcctttgtc
    31561 tatgctgcta taaatgcgct tatcaatggt gctccgggaa cgatggacac gctgaaagaa
    31621 attgcagcag ccatcaataa cgacccaaag ttcagcgaaa ctatcaataa cgcactggct
    31681 cttaaagctc ctttggcaag cccggcgttt acaggtacgc cgacagcgcc tacggcctca
    31741 caaggcacaa atagcacgca gattgcaaat acagcctttg ttaaggcagc tataacggca
    31801 cttatcaacg gtgcgcctgg cacattggat acgctgaaag aaatagcggc tgcgatcaat
    31861 aacgatccga atttcagcac aactatcaac aatgccctgg ctctcaaagc gcctttggca
    31921 agccctgcat taacgggtgt ccctactgcg ccgaccgcag cacagggcac aaataatacg
    31981 cagattgcta cgaccgctta tgtaagagct gccatatccg cattggttgg ttcatcacca
    32041 gaagctcttg ataccctgaa tgagcttgcc gcagcacttg gcaatgaccc gaactttgcg
    32101 acaacaatga caaatgcgct ggcaggcaaa cagcctctgg atgcaacttt aaccgcgctc
    32161 gctggccttg cgactggtgc aaacaaactg ccttatttca ccggtaagga tacggtagcg
    32221 cagactgatt taacgtcagt cggtcgcgat attctggcta aaacaagcac actagccgtt
    32281 atccaa
//
LOCUS       DAZFWP020000043        31887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFWP020000043 DAZFWP020000000
VERSION     DAZFWP020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.043"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(284..1657)
                     /gene="argH"
                     /locus_tag="ACHK0Z_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             complement(284..1657)
                     /gene="argH"
                     /locus_tag="ACHK0Z_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU4501873.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1718..2494)
                     /gene="argB"
                     /locus_tag="ACHK0Z_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             complement(1718..2494)
                     /gene="argB"
                     /locus_tag="ACHK0Z_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU4501874.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2502..3506)
                     /gene="argC"
                     /locus_tag="ACHK0Z_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             complement(2502..3506)
                     /gene="argC"
                     /locus_tag="ACHK0Z_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU4501875.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3660..4811
                     /gene="argE"
                     /locus_tag="ACHK0Z_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             3660..4811
                     /gene="argE"
                     /locus_tag="ACHK0Z_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU4501876.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5163..7814
                     /gene="ppc"
                     /locus_tag="ACHK0Z_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             5163..7814
                     /gene="ppc"
                     /locus_tag="ACHK0Z_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU4501877.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7997..9730
                     /gene="eptC"
                     /locus_tag="ACHK0Z_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             7997..9730
                     /gene="eptC"
                     /locus_tag="ACHK0Z_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4501878.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9879..10730
                     /gene="yijO"
                     /locus_tag="ACHK0Z_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             9879..10730
                     /gene="yijO"
                     /locus_tag="ACHK0Z_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4501879.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10717..11058)
                     /gene="frwD"
                     /locus_tag="ACHK0Z_RS09245"
                     /old_locus_tag="pgaptmp_001849"
     CDS             complement(10717..11058)
                     /gene="frwD"
                     /locus_tag="ACHK0Z_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4501880.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11060..11938)
                     /gene="pflC"
                     /locus_tag="ACHK0Z_RS09250"
                     /old_locus_tag="pgaptmp_001850"
     CDS             complement(11060..11938)
                     /gene="pflC"
                     /locus_tag="ACHK0Z_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU4501881.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(11904..14201)
                     /gene="pflD"
                     /locus_tag="ACHK0Z_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             complement(11904..14201)
                     /gene="pflD"
                     /locus_tag="ACHK0Z_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4501882.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14252..14572)
                     /gene="frwB"
                     /locus_tag="ACHK0Z_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             complement(14252..14572)
                     /gene="frwB"
                     /locus_tag="ACHK0Z_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4501883.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14587..15666)
                     /gene="frwC"
                     /locus_tag="ACHK0Z_RS09265"
                     /old_locus_tag="pgaptmp_001853"
     CDS             complement(14587..15666)
                     /gene="frwC"
                     /locus_tag="ACHK0Z_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU4501884.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15975..18476
                     /gene="ptsP"
                     /locus_tag="ACHK0Z_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             15975..18476
                     /gene="ptsP"
                     /locus_tag="ACHK0Z_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4501885.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18488..19150
                     /gene="fsa"
                     /locus_tag="ACHK0Z_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             18488..19150
                     /gene="fsa"
                     /locus_tag="ACHK0Z_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4501886.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGIVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19161..20264
                     /gene="gldA"
                     /locus_tag="ACHK0Z_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             19161..20264
                     /gene="gldA"
                     /locus_tag="ACHK0Z_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU4501887.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20539..21156
                     /gene="yijF"
                     /locus_tag="ACHK0Z_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             20539..21156
                     /gene="yijF"
                     /locus_tag="ACHK0Z_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU4501888.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21183..22088)
                     /gene="yijE"
                     /locus_tag="ACHK0Z_RS09290"
                     /old_locus_tag="pgaptmp_001858"
     CDS             complement(21183..22088)
                     /gene="yijE"
                     /locus_tag="ACHK0Z_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU4501889.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22182..24362)
                     /gene="katG"
                     /locus_tag="ACHK0Z_RS09295"
                     /old_locus_tag="pgaptmp_001859"
     CDS             complement(22182..24362)
                     /gene="katG"
                     /locus_tag="ACHK0Z_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU4501890.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24691..25581)
                     /gene="metF"
                     /locus_tag="ACHK0Z_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             complement(24691..25581)
                     /gene="metF"
                     /locus_tag="ACHK0Z_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU4501891.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25657..25791
                     /locus_tag="ACHK0Z_RS09305"
                     /old_locus_tag="pgaptmp_001861"
     CDS             25657..25791
                     /locus_tag="ACHK0Z_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501892.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25930..28362)
                     /gene="metL"
                     /locus_tag="ACHK0Z_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             complement(25930..28362)
                     /gene="metL"
                     /locus_tag="ACHK0Z_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU4501893.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28365..29525)
                     /gene="metB"
                     /locus_tag="ACHK0Z_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             complement(28365..29525)
                     /gene="metB"
                     /locus_tag="ACHK0Z_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU4501894.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGYGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29802..30119
                     /gene="metJ"
                     /locus_tag="ACHK0Z_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             29802..30119
                     /gene="metJ"
                     /locus_tag="ACHK0Z_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU4501895.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30303..30911
                     /gene="yiiX"
                     /locus_tag="ACHK0Z_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             30303..30911
                     /gene="yiiX"
                     /locus_tag="ACHK0Z_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU4501896.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            31056..31731
                     /locus_tag="ACHK0Z_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /pseudo
     CDS             31056..31731
                     /locus_tag="ACHK0Z_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7709 a         8856 c         8086 g         7236 t
ORIGIN      
        1 aaaatgggca cagcaataga aaataattat attcaattaa ttaaaatatg tattggttga
       61 tgtatttgaa aatatgattt tcatggggag tgtgaaggta aagaagacac aatgataaaa
      121 aggaatgtga caaataatgg ttctggttgg gcgttaaatt taagctcacc agaatgctga
      181 caaatttctg cctgatgtgg cgtaaacgcc ttatccgcct acgaagtgtc gcactttgta
      241 ggcgtggtaa gctccccatt ttcaggggag caatataaac aaattactct aaccgcgcct
      301 gcgcaaaagc aattgcctgc gccacctgct gcggtgagac gccgcctttt gccgcacgct
      361 tgtcgaggca cgattgcagc gacagaatcg gatagacatc ttcgtcaatc accgggctga
      421 atttctgcaa ctcgtcgagc ggcagatctt ccagcggttt gccctgacga atggcttcca
      481 ccaccgcttc accaacaata tggtgcgcct cgcggaacgg tacgcctttc gccaccagat
      541 aatccgccag ttcggtggcg ttggcgtaac cctgctgagc cgcttcctgg caacgtggac
      601 gtttcacctg aatgccgtcc agcaccagcg ccgccatatg caggcagtcc agccaggtat
      661 cgagcgcgtc gaacagacct tctttgtctt cctgcatatc tttgttgtaa gccagcggca
      721 aacctttcag cgtcatcatc atgccggtta acgccccctg cacccgaccg catttaccgc
      781 gaatcagctc cagcgcatcc gggtttttct tctgcggcat taatgatgaa ccggaagtca
      841 cgcggtcaga aagctccaca aaccccgctt cgccggtgtt aaagaaaatc agatcttcag
      901 caaaacgcga cagatgcacc atgccgatag cggcagcaga aagcagttcc aacacatggt
      961 cacggtcaga aacgctgtcg agactgttac gggtcgccga agcaaagccc agccagcctg
     1021 ctaactgttc acggtcgatt tcataggccg ttcccgccag cgcgccacag cctagcgggc
     1081 tgacatccag acgcttaagc gcatcctgca aacggctttc atcacgcgcc agcatctcaa
     1141 cataggccag gcaccagtgc gcgaacgtca ccggctgggc gcgttgcagg tgagtgtaac
     1201 ctggcattac cgcgtcctga ttgttttgtg cggtttccac cagcgccgat tgcagctgcc
     1261 ggttagccgt cagtaactcg ctaacggtat ctttgcacca cagtttcagg tcagtcgcta
     1321 cctgatcatt acggctacgc ccggtatgca gctttttgcc taactggccc actttgtcga
     1381 tcagtttgcc ttccacccag ctatggatat cttcggcgtc gctttcaagg atttgttgtg
     1441 gcctggcgcg aacatcttcc agcaacacgt tcagcgcctc ttccagttgc gcctgctctt
     1501 ctgcggttaa cacgcctacc gtgaccaggg ctttggacca ggccacagag ccaacaatat
     1561 cctgctccgc cagacggtaa tcaaagcgca gtgagtcgtt gaattgtttg aaccgttgat
     1621 ctgctgcctg ggtaaaacgc ccgccccaaa gtgccataac tctgtttcct tattttgaaa
     1681 ttcaatgccg gaaagctgcg cctccggcca acaaaactta agctaaaatc cgcgtaccca
     1741 tcggcatacc gttaaacagt gccggaagct gctccgcatg acgccaggag gcgatatcta
     1801 ccggacggcc cagcgtgcgg gccgcatcca gcgccgcgtt cactttcact atcatgccgt
     1861 cagtaataat gccctgctca atcagttgtt ctgctttcgc ggcggtcatt tcggcaatgc
     1921 gttgcccttt gccgtcgaga atgccgctga cgtcggagag caaaatcaga tccgcgccca
     1981 gcgttgccgc cagcgccgtt gccgcctggt cggcattgac gttcatcagt tgcccttcgt
     2041 ctgttacgcc aatggagctg accaccggca gataaccgtt ctccagcaag gagttgataa
     2101 gcttaggcga acctggctgc gccagtccaa catgacctaa ctcttcatca agctgggtca
     2161 ctttgacgct gtcgccgtca ccgagaaaca aacctacggc cgcaatctga tgtttcttcg
     2221 cccatgccaa cagggtttta tttgccgttc ccgccagtgc tccggtgata atgtctatct
     2281 gatcagcagg cgtcacccgc aggccgtttt tctttttcac cggcagattc agccctttca
     2341 tcagctcatc caccacgcaa ccgccgccgt gcacaatcac cagcggacgc tgatgtgact
     2401 cacgataatt caccagtgcg ctaaacagac gttccagcgc ctcttcacta tccagcagta
     2461 cgccgcccag tttgataatt aatggattca tcattgcacc cttaaataag agactgcgtt
     2521 tccgcatagc cgaaacgaat attggcgcac tgtaccgctt gtgccgccgc gcctttcagt
     2581 aagttgtctt cggtcgccac aataatcaga tgctcgccct gaacggcaaa cccgatatcg
     2641 caaaatggca gcccaacgac atttttcagc gccggaacgc ctttgtcata cagccgcacc
     2701 agcggtttat gggcatacgc ctgctgtaac acttgcgcga cttgcgcctg ggtcacaccc
     2761 gatttcaggc ggcaggtaat ggtttcgaga atgccgcgcg ggaaattgcc cagatgtggg
     2821 gtgaagataa cgtcagcacc gaggtgtgtg gcgatctctg gttgatggcg atgagtaaag
     2881 acgccatacg gttgcaggct aacttcacaa aagctgtttg aaatggccgc tttacgccct
     2941 gcaccgctca cgccgctggt ggcgttgatc accggccact gattgaggtc aagaagatcg
     3001 gcatcaatca acggtttcag cgccagctgt gccgccgtcg gataacagcc cggcaccgca
     3061 atcaaattcg cttcttttaa tttattaccg caccactccg ccagaccgta ggctgcctgt
     3121 tccaacagtt ccgggtattg atgggtaaag ccgtaatatt tttcatagaa ggtggcgtcg
     3181 ttaacacgaa acgcgccgga aaggtcgaac accacgcagc ccgcttcaag aaactgcggc
     3241 gctaaatcgt ggctaacttc atgggcggtg gcgagaaaca ctacgtccac ccctgggcta
     3301 aactcgctga tatccgacat cggctgcaac ggcagatcaa cgatgccttt tagctgcgga
     3361 tgcaaatcgg agattaactt gcccgcatca ttgctttgcg ctgaaacagt caaagcggtt
     3421 atgttcatat gcggatggcg atttacatag gtcactagct ctgcgccagc gtagccgctg
     3481 gcacccacaa tcagcgtatt caacatcggg gctattcacc ttcttatgtc tggttgccag
     3541 gttaaacgta aaacattcac cttacggctg gtgggtttta ttacgctcaa cgttagtgta
     3601 tttttattca taaatactgc atgaatattg atactatcat gaccagaggt gtgtcaacaa
     3661 tgaaaaacaa attaccgcca tttatcgaga tttaccgtgc tctgattgcc acaccttcaa
     3721 taagcgccac ggaagaggca ctcgatcaaa gcaatgcaga tttaatcact ctgctggcgg
     3781 actggtttaa agatttaggc ttcaatgtgg aagtgcagcc tgtgccagga actcgcaaca
     3841 aatttaatat gctggcaagt atcggacagg gggctggcgg cttgttgctg gcggggcata
     3901 ccgatacggt accatttgat gacggtcgct ggacgcgtga tccgtttaca ctgacggagc
     3961 atgacggcaa gctttacggc ttaggcaccg ccgacatgaa aggctttttt gcgtttatcc
     4021 ttgatgcgct acgcgatgtc gacgtcacga aactgaaaaa accgctctac attctggcga
     4081 ctgctgatga agaaaccagt atggccggag cgcgttattt tgccgaaact accgccctgc
     4141 gcccggattg cgccatcatt ggcgaaccga cgtcactaca accggtacgc gcacataaag
     4201 gtcatatctc taacgccatc cgtattcagg gccagtcggg gcactccagc gatccagcac
     4261 gcggagttaa cgctatcgaa ctaatgcacg acgccatcgg gcatattttg caattgcgcg
     4321 ataacctgaa agaacgttat cactacgaag cgtttaccgt gccataccct acgctcaacc
     4381 tcgggcatat tcacggtggc gacgcttcta accgtatttg cgcttgctgt gagttgcata
     4441 tggatattcg tccgctgcct ggcatgacac tcaatgaact taatggtttg ctcaacgatg
     4501 cattggctcc ggtgagcgaa cgctggccgg gtcgtctgac ggtcgacgag ctgcatccgc
     4561 cgatccctgg ctatgaatgc ccaccgaatc atcaactggt tgaagtagtt gagaaattgc
     4621 tcggagcaaa gaccgaagtg gtgaactact gtaccgaagc gccgtttatt caaacgttat
     4681 gcccgacgct ggtgttgggg cctggctcaa ttaatcaggc tcatcaacct gatgaatatc
     4741 tggaaacacg gtttatcaag cccacccgcg aactgataac ccaggtaatt caccattttt
     4801 gctggcatta aaacgtaggc cggataaggc gctcgcgccg catccggcac tgttgtcaaa
     4861 ctccagtgcc gcaataatgt cggatgcgat acttgcgcat cttatccgac ctacaccttt
     4921 ggtgttactt ggggcgattt tttaacattt ccataagtta cgcttattta aagcgtcgtg
     4981 aatttaatga cgtaaattcc tgctatttat tcgtttgctg aagcgatttc gcagcatttg
     5041 acgtcaccgc ttttacgtgg ctttataaaa gacgacgaaa agcaaagccc gagcatattc
     5101 gcgccaatgc gacgtgaagg atacagggct atcaaacgat aagatggggt gtctggggta
     5161 atatgaacga acaatattcc gcattgcgta gtaatgtcag tatgctcggc aaagtgctgg
     5221 gagaaaccat caaggatgcg ttgggagaac acattcttga acgcgtagaa actatccgta
     5281 agttgtccaa atcttcacgc gctggcaatg atgctaaccg ccaggagtta ctcaccacct
     5341 tacaaaattt gtcgaacgac gagctgctgc ccgttgcgcg tgcgtttagt cagttcctga
     5401 acctggccaa caccgccgag caataccaca gcatttcgcc gaaaggcgaa gctgccagca
     5461 acccggaagt gatcgcccgc accctgcgta aactgaaaaa ccagccggaa ctgagcgaag
     5521 acaccatcaa aaaagcagtg gaatcgctgt cgctggaact ggtcctcacg gctcacccta
     5581 ccgaaattac ccgtcgtaca ctgatccaca aaatggtgga agtgaacgcc tgcttaaaac
     5641 agctcgataa caaagatatc gctgactacg aacacaacca gctgatgcgt cgcctgcgcc
     5701 agttaatcgc ccagtcatgg cataccgatg aaatccgtaa gctgcgtcca agcccggtag
     5761 atgaagccaa atggggcttt gccgtagtgg aaaacagcct gtggcaaggc gtaccaaatt
     5821 acctgcgcga actgaacgaa caactggaag agaacctcgg ctacaaactg cccgtcgaat
     5881 ttgttccggt ccgttttact tcgtggatgg gcggcgaccg cgacggcaac ccgaacgtca
     5941 ctgccgatat cacccgccac gtcctgctac tcagccgctg gaaagccacc gatttgttcc
     6001 tgaaagatat tcaggtgctg gtttctgaac tgtcgatggt tgaagcgacc cctgaactgc
     6061 tggcgctggt tggcgaagaa ggtgccgcag aaccgtatcg ctatctgatg aaaaacctgc
     6121 gttctcgcct gatggcgaca caggcatggc tggaagcgcg cctgaaaggc gaagaactgc
     6181 caaaaccaga agggctgctg acacaaaacg aagaactgtg ggaaccgctc tacgcttgct
     6241 accagtcact tcaggcgtgt ggcatgggta ttatcgccaa cggcgatctg ctcgacaccc
     6301 tgcgccgcgt gaaatgtttc ggcgtaccgc tggtccgtat tgatatccgt caggagagca
     6361 cccgccatac cgaagcgctg ggcgagctga cccgctacct cggtatcggc gactacgaaa
     6421 gctggtcaga ggccgacaaa caggcgttcc tgatccgcga actgaactcc aaacgtccgc
     6481 ttctgccgcg caactggcaa ccaagcgccg aaacgcgcga agtgctcgat acctgccagg
     6541 tgattgccga agcgccgcaa ggctccattg ccgcctacgt gatctcgatg gcgaaaacgc
     6601 cgtccgacgt actggctgtc cacctgctgc tgaaagaagc gggtatcggg tttgcgatgc
     6661 cggttgctcc gctgtttgaa accctcgatg atctgaacaa cgccaacgat gtcatgaccc
     6721 agctgctcaa tattgactgg tatcgtggcc tgattcaggg caaacagatg gtgatgattg
     6781 gctattccga ctcagcaaaa gatgcgggag tgatggcagc ttcctgggcg caatatcagg
     6841 cacaggatgc attaatcaaa acctgcgaaa aagcgggtat tgagctgacg ttgttccacg
     6901 gtcgcggcgg ttccattggt cgcggcggcg cacctgctca tgcggcgctg ctgtcacaac
     6961 cgccaggaag cctgaaaggt ggcctgcgcg tgactgaaca gggcgagatg atccgcttta
     7021 aatatggtct gccagaaatc accgtcagca gcctgtcgct gtacaccggg gcgattctgg
     7081 aagccaacct gctgccacca cctgagccga aagagagctg gcgtcgcatt atggatgaac
     7141 tgtcagtcat ctcctgcgat ctctaccgtg gctacgtacg tgaaaacaaa gattttgtgc
     7201 cttacttccg ctccgctacg ccggaacaag aattgggcaa actgccgttg ggttcacgtc
     7261 cggcgaaacg tcgcccaacc ggcggcgtcg agtcgctgcg cgccataccg tggatttttg
     7321 cctggacgca aaaccgcctg atgctccccg cctggctggg tgcaggtacg gcgctgcaaa
     7381 aagtggtcga agatggcaaa cagagcgagc tggaagccat gtgccgcgat tggccattct
     7441 tctcgacgcg tctcggcatg ctggagatgg tcttcgccaa agcagacctg tggctggcgg
     7501 aatactatga ccaacgcctg gtagacaaag cactgtggcc gttaggtaaa gagttacgca
     7561 acctgcaaga agaagacatc aaagtggtgc tggcgattgc caacgattcc catctaatgg
     7621 ccgatctgcc gtggattgca gagtctattc agctacggaa tatttacacc gacccgctga
     7681 acgtattgca ggccgagtta ctgcaccgct cccgccaggc agaaaaagaa ggccaggaac
     7741 cggatcctcg cgtcgaacaa gcgttaatgg tcactattgc cgggattgcg gcaggtatgc
     7801 gtaataccgg ctaatcttcc tcttctgcaa accctcgtgc ttttgcgcga gggttttctg
     7861 aaatacttct gttctaacac cctcgttttc aatatatttc tgtctgcatt ttattcaaat
     7921 tctgaatata ccttcagata tccttaagga attgtcgtta cattcggcga tatttttttc
     7981 aagacaggtt cttactatgc attccacaga agtccaggct aaaccccttt ttagctggaa
     8041 agccctgggt tgggcactgc tctacttttg gtttttctct actctgctac aggccattat
     8101 ttacatcagt ggttatagtg gcaccaacgg cattcgcgac tcgctgttat tcagctcgct
     8161 gtggttgatc ccggtattcc tctttccgaa gcggattaaa attattgccg cagtaatcgg
     8221 cgtggtgcta tgggcggcct ctctggcggc gctatgctac tacgtcatct acgggcagga
     8281 gttctcgcag agcgttctgt ttgtgatgtt cgaaaccaac accaacgaag ccagcgagta
     8341 tttaagccag tatttcagcc tgaaaattgt gcttatcgcg ctggcctata cggcggtggc
     8401 agttctgctg tggacacgcc tgcgcccggt ctatattcca aagccgtggc gttatgttgt
     8461 ctcttttgcc ctgctttatg gcttgattct gcatccgatc gccatgaata cgtttatcaa
     8521 aaacaagccg tttgagaaaa cgttggataa cctggcctcg cgtatggagc ctgccgcacc
     8581 gtggcaattc ctgaccggct attatcagta tcgtcagcaa ctaaactcgc taacaaagtt
     8641 actgaatgaa aataatgcct tgccgccact ggctaatttc aaagatgaat cgggtaacga
     8701 accgcgcact ttagtgctgg tgattggcga gtcgacccag cgcggacgca tgagtctgta
     8761 cggttatccg cgtgaaacca cgccggagct ggatgcgctg cataaaaccg atccgaatct
     8821 gaccgtgttt aataacgtag ttacgtctcg tccgtacacc attgaaatcc tgcaacaggc
     8881 gctgaccttt gccaatgaaa agaacccgga tctgtatctg acgcagccgt cgctgatgaa
     8941 catgatgaaa caggcaggtt ataaaacctt ctggatcacc aaccagcaga cgatgaccgc
     9001 ccgcaatacc atgctgacgg tattttcgaa gcagaccgac aaacagttct atatgaacca
     9061 gcaacgtacg cagagtgcgc gtgaatacga caccaacgtg ctgaaaccat tccaggaagt
     9121 gctgaaggac ccggcgccga agaaactgat catcgttcat ctgctgggta cgcatatcaa
     9181 atacaaatac cgctacccgg aaaatcaggg caagtttgat ggcaataccg atcatgttcc
     9241 gcctggatta aacgcggaag agctggagtc atataacgat tatgacaacg ctaacctgta
     9301 taacgatcat gtggttgcca gcctgattaa agactttaaa gcagcaaacc cgaacggttt
     9361 cctggtttat ttctctgacc acggtgaaga ggtttacgac acgccgccgc acaaaactca
     9421 ggggcgtaac gaagacaacc cgacgcgcca catgtacacc attccgttcc tgctgtggac
     9481 gtcggaaaaa tggcaagcga ctcatccgcg tgatttctcg caggatgttg atcgtaaata
     9541 cagcctggcg gaactgatcc acacctggtc agatttagca ggcttatctt acgacggtta
     9601 cgatccaacc cgttcagtgg tgaatccgca gttcaaagaa actacccgct ggattggtaa
     9661 cccgtacaag aaaaacgcgc tgatcgatta cgacacgctg ccgtatggcg atcaggtggg
     9721 taatcagtaa atgacctttg tatcgcgtct ggcgctattt gccggatgcg atactgccgc
     9781 aatgtttaca tcgaactccc ctcaggccac acttttcagg tggccttttt taataatgcc
     9841 agttactgtt ttcccgtcta catacctaag ggaataacat gtatcacgac gtcagctatc
     9901 tgctctcccg cctgatcaac ggcccgctgt ccctgcgcca gatttacttt gccagcagta
     9961 acggtccggc gcccgatctt gcgtatcagg tcgattttcc acggctggaa atagtgctgg
    10021 aaggtgagtt tgttgatacc ggcgctggag caacgttagt tcccggcgat gtgctgtacg
    10081 ttcccgctgg tggctggaat tttccacaat ggcaagcccc cgctactacc tttagcgtgc
    10141 tgtttggcaa acagcaactc ggcttcagcg tcgtgcaatg ggatggcaaa cagtatcaaa
    10201 atctggcgaa gcaacacgtc gcccgacgcg gcccgcgcat tggttctttt ctgctacaaa
    10261 cgctcaatga aatgcaaatg cagccacagg agcagcaaac ggcaaggctt atcgtcgcca
    10321 gcctgcttag ccactgccgc gatttgctcg gcagccaaat acagaccgcc tcacgcagcc
    10381 aggctctatt cgaagctatt cgtgattata tcgacgaacg ctatgcctcc gcgcttaccc
    10441 gcgaatctgt cgcacaggcg ttctatattt caccaaacta tctctcgcac ctgtttcaaa
    10501 agacgggggc cattggtttt aacgaatacc tgaatcacac gcgactggag cacgctaaga
    10561 cgttattgaa aggctatgac ctgaaggtaa aagaagtggc gcacgcctgc ggctttgtcg
    10621 acagcaacta cttctgccgt ctgtttcgta aaaacaccga acgctcgccg tcggaatatc
    10681 gtcggcagta ccacagccaa ctgacagaaa aaccgactac tccagaataa gatgggtttg
    10741 ctgcggcgca gaaagcactt tacgtaccgc gctcatcacc cgctgcggct cacgcaggaa
    10801 cgcgtagata ctgcattgca cgtagcgaca atgttcaaat cgctcggcac cagcaagctc
    10861 gatatccgta atcaacagcg caacatccgc ccggcggata tcctcgtccg ctaaacgatt
    10921 ctccgttccc agcgcgccct gagtttcaat gctgacgccc cacttctcta actggcacaa
    10981 cttttccagc cgttccgccg ccatataagt atgcgccacg ccactgacgc aggcggttac
    11041 tgccaccagg tatgccattt taacctccca cggtaacctg aaatccggcc cgttctgcca
    11101 tttcgcgcat cgttgccaca tcggctgacg acggcgcaga aacctctttc atcgaccatg
    11161 ttttccccag cagacggtat ttcggttcgc cgtactgatg aaacggtaac agatggatct
    11221 gcctgatatt cagcgggatc agcacgtcca gcgcctgctg catattctcc cggctgagtg
    11281 tgaaaccggg gatcagcggc aaacgcggga tcacgttgac accttcactc accagcaaac
    11341 gcagattctc cagcacgcgt ggcaggttca tcttcaccac atcccgcgcc tgagtcgcgt
    11401 ccataatttt taaatcgaac aacacttcat cgcacaattt cgccagcggt aacagcttgc
    11461 tggctggcgc gtctccggca gtttcgatgg cgcatgacac gccccacagc cgcagtcgct
    11521 gtaaaaaacg ggtagcaaac tcggcctgca ttaacacttc gccgccagaa agcgtgacgc
    11581 cgccgccgga cgtgcgaaag aaaatgtcat ctttcatcac ttcccgttcc agagcgtcaa
    11641 ggctgatatc gcgaccaatc cgttcaaacg caccggaggg gcattcatcc gcatcacgca
    11701 aacatttcgc acagtgcaga catttcgcct ctctgcgtac cgtctggatt ttgccggaga
    11761 tcgactccgg attagcacac cacgggcaaa gatgcggaca gcctttaaaa aagaccaccg
    11821 tacgaatgcc ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat
    11881 catcgcggcg cgtttccaca acgttacagc tgatgcgctg tccggcggat gatgtcatcc
    11941 tggatctcct tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg caccaccagc
    12001 ccggcataat cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt caccacgtta
    12061 aactgaatat gctgcaactt aagctgggta aacgcccgta agaagtcggc cagtttgcgt
    12121 aatcccgctt caccttccag ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta
    12181 gacagcagcg tgttatcgag cttactgact gacttcagta ccgctgttgg cccttgtgcg
    12241 tcctggccca gcataggtga caagccgccg tctgccagtt gttccccggc aaaacgaccg
    12301 tctggcgtcg cgccaaccac cgatcccaac gggacgtgag cagaaacggt atacgatccc
    12361 ggcgtgaagt aaccgccgcg cgggttctgg tatttttcca cttctttgca gtagtggcgt
    12421 aacagttcgg cgctgatgtt atccacctcg tcgatatcgt taccgtattt ctcgaagcgg
    12481 ttaattaagc gagcacggac tttttcgcct tctggcgttg caaagttggc ttttaatacc
    12541 gacagcaatt cgtcaaaact taaacgctgt tgctcaaaaa ccatcccttt caacgcatgg
    12601 agagaatcgc tcaggttggc gataccgatc ccctgtacgc cggagaagtt ataacgcgcg
    12661 ccgccatcgg taatatcgcg acctttttcc agacaatcgc tgataaaaga cgagagcagc
    12721 ggtacaggtg cccagtcgcg atggccgata tcacaaatat tgctgccctc aaccatcagg
    12781 gtgatgtagt ggctgatctt ggcgcggatc tgctccagta aaccttcata agtcagcgca
    12841 gcattgcctt cattttcatg caggcagatt tccatcactt tcagcagatt aaacatcgcg
    12901 atgtcatgca agccgtaggt tctgccggga atagataatt ccacacagcc cactacggaa
    12961 tagtcgcgcg catcttccag cgaaacgcca cggttgagga acgctggtac caccacttca
    13021 tcgttaaaga tttgcggaat accggtgccg aggcgaatgg tttcggcggt tttcatcagg
    13081 aacggcgtgt cgatcaaggc gttagtgcgc acgccgaggt tcggttgcgg taattgcacg
    13141 ctttgataag cgtcaaggca aaggaacgaa agcacgttca ccgcgctacg tccgttctcg
    13201 gttaacccgc cgagcagtgc ggtatagccg gtcgggaaac ctgcgaaata acgcgcgctg
    13261 ctggtggagc gcaacagcac gatgtcgttg catttcaccc ataaagattc gagtagttct
    13321 ttcaggaacg ccggatcttc gccctgggtt aaagatgcct gatagaacgg caacatatac
    13381 tggtcgaagc gccccaacga tagcgaactg gcgttggatt cgtattgcag aatgatgttc
    13441 atgtaccaga ataactggca cgcctgccag aacgtctgcg gcttatgttc ggcgttatgg
    13501 cgggagatct ccgcaatagt cagcagttct tcgcgacgct gggcatctgt gcagtttgcc
    13561 gccattgttt ccgccagttc agcgtaacgc agaatgtgtt tctgcgaggc ttccagcagt
    13621 aacagcgcgg cctgataaaa gtgattctcc ggctgttgct gacaatgttg ctgcatctgc
    13681 gccaccagct cccccagccc gtgattcagc aggcgtgggt aatcaataat aatgtgcccc
    13741 tggcctttat ctgtctggtt gatgctgaaa atctgcgtgc tggtcgcggc ttttacttca
    13801 tctgtcatct gcccgttgat gaaatctttc atcgaacgtt tttcccagta cgggaacaac
    13861 tcttcacggt agatacgttt atcttcttca ctgatagcaa agcggtcctg cggacgcgtc
    13921 gggaattgat ccagctcttt cagcagccag taagggtcca tttccggcga cataatccca
    13981 gcgcgcggtt ttacggtgcg gttaccggca atcagttctt catcacgaat cgaaatttca
    14041 acatgttcaa ggatatacgc tgttgctttc gcccggcgca atatcaccgg ttcgccttcg
    14101 gtttgccgat ggctggcggt ataaagcagc gcccgctcca gcgagatttc acgggtattg
    14161 gcaaacagtg cagttttgag gcgagagata cgattcgtca tgaggaactc ctgtgggaaa
    14221 gcgaacccgc ccttgcgagc gggagaagaa cttaagcggt ttgcgccagg tgagcttcaa
    14281 ttttgctcat aatggcgtca gcacgtttta ctgcatcgct gatgttgacg cgcacgatgg
    14341 tcttgccagc aaaacgctct tcaaacttga tgccgatatc tttggtcaga atcaccatat
    14401 cagcgctcgc cacatcttcc gcagtcagtt cattttccag accaattgac ccctgggttt
    14461 ctactttcac ttcccagcct ttcgctttcg cggcactttc cagcgcctct gctgccatat
    14521 aggtatgggc aacacctgaa ggacatgcgg ttactgcaat aattttcgtc atggctcgtt
    14581 cctcaattaa ttaatttcaa aatccaaatc caggtcgtct tctttttcat cagtcgaaga
    14641 cccattttta cgcgccagac ttttcagcac gttcacacaa acagccgtca ccactgctcc
    14701 gacagccact gctgcgatat aacccagctt gccttcaacc accggcagca caatcagacc
    14761 accccagcct gcatagcact gcgcaccgac cagcgccgca gttactgcac cacaaactga
    14821 accgaccatg atggaaggaa tgacacgcag cggatcggca gcggcgaaag gaatcgcccc
    14881 ttcagtaacg ccaacacagc ccatcaccag tgccgctttg ccggtttcgc gctcttctgc
    14941 ggagaaattt ttacgaccaa tcagcgtcgc cagccccatc cccagcggcg ggatacaaat
    15001 acccacggca gcgatggcca ccacggtata aacaccctga gcaacgcaaa tcagcatgaa
    15061 ggcataggcc actttgttaa ccggaccgcc catatcgaac gccagcatca gacccatgat
    15121 caccgccagc ataacaatgc tgccctgctg catcccctga agccactgag tcaggctgtt
    15181 ggtcaacgcc cctacaggct cgcccaagcc ccacatcatg atgcctgcgg taatcagtgt
    15241 gccgacgata ggaataatga agataggcat caccgagcgc agaactttat gcaccggaat
    15301 tttcttcagg taatgcacca cgatgccgcc gataatcccg gcgatcagtg caccaaagaa
    15361 gcccgcacca aagctgttac caacccaggc accgatagcg cacggagcca gcgcagaacg
    15421 ctctgcaatg gaataaccga tgtacgctgc gaggaaaggc accatcagcg tcaagcccgc
    15481 aacgccgata tcaaacagtt ttttcagatt tggatcggct acggcatccg gcactgcgcc
    15541 tttgccatac aacatgacgg aaaccgccag caaaataccg cccgatacca cgaagggaat
    15601 catgtgtgaa acgcccgtca ttaaatgctg acgggtattt ttcaggatct gcaccaactc
    15661 attcataagc tgctcccggg cttgcgaaag cccatgtcca tatttttgat ggaggaaaca
    15721 ataggcaatc gcaggcggag caaacagtgg ataaaaaggc catttactgg acttttgtaa
    15781 tatcagtaca aaaatgcgat ccgcctcata acttgcgata aaccggaagc atgccgcaaa
    15841 acagcgtctt gtgagacgcg tcgcaaattg acggttacat tacatttgtc cagcttttgg
    15901 cagatttgtc acatggcagg ccgttgccgt gcgctcttat cctgtaaaca gaaaaccgta
    15961 ccgggagaga ggcaatggcc ctgattgtgg aatttatttg tgagctacct aacggcgtac
    16021 atgcacgtcc ggcaagccac gttgaaacgc tgtgtaatac tttttcatca caaattgagt
    16081 ggcataacct gcgcactgac cgcaagggca acgccaaaag cgcccttgcg ctgattggca
    16141 ccgatacgct ggcgggcgat aactgccagt tactgatttc cggggccgac gaacaggaag
    16201 cgcaccagcg tttaagccaa tggctgcgcg atgaattccc ccactgcgat gcgccgctgg
    16261 cggaagttaa atctgacgaa ctggaaccac tgccggtttc actgaccaat ctgaatccgc
    16321 aaattatccg cgcccgcacc gtgtgcagcg gtagtgcagg cggcattctg acgccgatct
    16381 cttctttaga tctcaatgcg ctgggtaatc ttcccgcagc caaaggcgtt gacgccgagc
    16441 aatccgcact ggaaaacggc ctgacgctgg tactgaaaaa cattgagttt cgtctgctgg
    16501 atagcgacgg tgccaccagc gcgattctgg aagcgcaccg atccctggct ggcgatactt
    16561 ccctgcgcga acatttactg gcaggcgtca gcgccggatt aagctgcgcc gaagcgattg
    16621 tcaccagtgc gaatcacttt tgcgaagagt tcgcccgttc cagcagcagc tacctgcaag
    16681 aacgtgccct ggacgtacgc gacgtctgct tccagttact ccagcaaatc tacggtgagc
    16741 aacgcttccc ggcaccgggc aaactgacgc agcccgccat ttgtatggct gatgaactga
    16801 ctcccagcca gttcctcgaa ctggataaaa atcacctcaa aggtctgtta ctgaaaagtg
    16861 gcggcaccac ctcacatacc gtaattcttg cccgttcgtt caacattccg acactggttg
    16921 gtgtagatat tgatgccctt actccgtggc agcatcaaac gatttatatc gacggcaacg
    16981 ctggggcgat tgtggttgag ccaggagaag ccgtagctcg ttattatcag caagaagccc
    17041 gcgtacagga cgccctgcgt gagcaacagc gtgtctggct gacccaacaa gcccgtaccg
    17101 ctgacggtat ccgcattgaa attgccgcta acatcgctca ctccgtggaa gcgcaggccg
    17161 cattcggcaa tggtgcggaa ggcgttggtt tgttccgcac tgaaatgctc tatatggatc
    17221 gcaccagtgc accgggcgaa agcgagctgt acaacatttt ttgtcaggcg ctggagtctg
    17281 ccaacggacg cagcattatt gtgcgcacta tggacattgg cggcgacaaa cccgttgatt
    17341 atctgaacat tccggcagaa gctaacccgt tcctcggtta tcgcgctgtg cgtatttatg
    17401 aagaatacgc ctcgctgttc accacacaac tacggtcgat cctgcgcgcc tccgctcacg
    17461 gcagcctgaa aatcatgatc ccgatgatct cctcaatgga agagatctta tgggtgaaag
    17521 aaaaactggc ggaagccaaa cagcaactgc gtaacgaaca cattccgttt gatgagaaga
    17581 tccagcttgg cattatgctg gaagtaccgt cggtgatgtt catcatcgat caatgctgcg
    17641 aagagattga tttctttagt attggtagta atgacctgac gcaatatctg ctggcagtgg
    17701 atcgcgataa cgctaaggtt actcgtcact acaacagcct gaatccggca ttcttgcggg
    17761 cgctcgatta cgccgtgcag gcggtgcatc gccagggcaa atggattggt ctgtgcggtg
    17821 agctgggagc gaaaggttcc gtgctgccgt tgctggtcgg cttagggctg gatgaactca
    17881 gcatgagcgc accatcaatt ccggcggcga aagcgcggat ggcgcaactt gatagccgtg
    17941 agtgccgcaa gttgctcaac caggcaatgg cctgccgtac ttcgctggaa gtggaacacc
    18001 tgctggcgca attccgcatg acccaacagg acgcaccgct ggtcaccgcc gagtgcatca
    18061 cgctggaaag tgactggcgc agcaaagaag aagtgctcaa aggcatgacc gataacctgc
    18121 tgctggcggg ccgctgccgc tatccgcgta aactggaagc cgacttatgg gcgcgcgagg
    18181 ccgttttctc taccggtctg ggctttagtt ttgccattcc acacagcaaa tcagaacaca
    18241 ttgagcaatc caccatcagc gtggcgcgtc tgcaagcgcc ggtgcgctgg ggcgatgatg
    18301 aagcgcaatt catcattatg ttaaccctga acaaacacgc tgcgggcgat cagcacatgc
    18361 gcattttctc gcgcctcgct cgccgcatca tgcacgaaga attccgtaac gcgctggtta
    18421 acgccgcctc tgccgacgct atcgccagcc tgctgcaaca tgaactggaa ctgtaaaagg
    18481 aaacatcatg gaactgtatc tggacaccgc taacgtcgca gaagtcgaac gtctggcacg
    18541 catattcccc attgccgggg tgacaactaa cccgagcatt atcgctgcca gcaaggagtc
    18601 catatgggaa gttctgccgc gtctgcaaaa agcgattggt gatgagggca ttctgtttgc
    18661 tcagactatg agtcgtgacg cgcagggtat agtggaagaa gcgaagcgcc tgcgcgacgc
    18721 cattccgggc attgtggtga aaatcccggt gacttccgaa ggtctggccg ccattaagat
    18781 gctgaaaaaa gagggtatta ctacgcttgg caccgcagtg tacagtgctg cacaagggtt
    18841 actggccgcg ctggcagggg caaaatacgt tgctccgtat gttaaccgcg tagatgccca
    18901 gggcggggac ggcattcgta cggttcagga gctgcaagcg ctgttagaaa tgcacgcacc
    18961 agaaagcatg gtgctggcgg ccagctttaa aacgccgcgt caggcgctgg actgtttact
    19021 ggcaggatgt gaatccatca ccctgccctt agatgtagcg caacaaatgc tcaacacccc
    19081 tgcggtagag tcagctatag agaagttcga acacgactgg aatgccgcat ttggcactac
    19141 tcatctctaa aggagcaatt atggaccgca ttattcaatc accgggtaaa tacatccagg
    19201 gcgctgatgt gattaatcgt ctgggcgaat acctgaagcc gctggcagaa cgctggttag
    19261 tggtgggtga caaatttgtt ttaggttttg ctcaatccac tgtcgagaaa agctttaaag
    19321 atgctggact ggtagtagaa attgcgccgt ttggcggtga atgttcgcaa aatgagatcg
    19381 accgtctgcg tggcatcgcg gagactgcgc agtgtggcgc aattctcggt atcggtggcg
    19441 gaaaaaccct cgatactgcc aaagcactgg cacatttcat gggtgttccg gtagcgatcg
    19501 caccgactat cgcctctacc gatgcaccgt gcagcgcatt gtctgttatc tacaccgatg
    19561 agggtgagtt tgaccgctat ctgctgttgc caaataaccc gaatatggtc attgtcgaca
    19621 ccaaaatcgt cgcgggcgca cctgcacgtc tgttagcagc gggtatcggc gatgcgctgg
    19681 caacctggtt tgaagcgcgt gcttgctctc gtagcggcgc gaccaccatg gcgggcggca
    19741 agtgcaccca ggcggcgctg gcactggctg aactgtgcta caacaccctg ctggaagaag
    19801 gcgaaaaagc gatgcttgcc gccgaacagc atgtagtaac cccggcgctg gagcgcgtga
    19861 ttgaagcgaa cacctatttg agcggcgttg gttttgaaag cggcgggctg gcggcggctc
    19921 acgcggtgca taacggcctg accgctatcc cggacgcgca tcactattat cacggtgaaa
    19981 aagtggcatt tggtacgctg acgcagctgg ttctggaaaa cgcaccggtt gaggaaatcg
    20041 aaaccgtagc tgcacttagc catgcggtag gtttgccaat aactctcgct caactggata
    20101 ttaaagaaga tgtcccggcg aaaatgcgaa ttgtggcaga agcggcatgt gcagaaggtg
    20161 aaaccattca caacatgcct ggcggcgcga cgccagatca ggtttacgcc gctctgctgg
    20221 tagccgacca gtacggtcag cgtttcctgc aagagtggga ataacctact ccaaactccc
    20281 ggcttgtccg ggagtttgaa cgcaaaattg cctgatgcgc tacgtttatc aagcctacga
    20341 aatctctgca atatattgaa tatgcgtgct tttgtaggcc ggataaggcg ttcactccgc
    20401 atccggcact ctgtgccagt aatctgaaac tcccacgttg ttcaggagtt ttttatccgc
    20461 ttctggacat tttctccaca gaacaatcgt tagcccggaa ggtcgaatct gtgactctta
    20521 ttttcacgga cgaaccagat gaaagcttca ctggcgctgc tcagcctgct aacggcattt
    20581 accagccact ccttaaaatc tcccgccgtt ccaccaaccg tggtacaaat ccaggccaat
    20641 accaaccttg ccatcgcaga cggtgccaga cagcaaatcg gcagcacgct attttacgat
    20701 ccggcgtata tgcagcttac ctatcctggc ggtgatgtcc cgcaagaacg cggggtatgt
    20761 tccgatgtgg tgatccgtgc attgcgcagc cagaaggtcg atttgcagaa actggtgcac
    20821 gaagatatgg cgaagaattt tgccgagtac ccgcaaaaat ggcagttaaa gcgcccggac
    20881 agcaacatcg atcaccgtcg ggtgcctaat ctggaaacct ggtttacccg ccacgataaa
    20941 acacgcccca tcagcaagaa ccccagtgac tatcaagcgg gcgatattgt ctcctggcga
    21001 ctggataacg gactggcaca tattggggtg gtgtcagatg gcttcgcccg cgacggtacg
    21061 ccgctggtga tacataacat tggcgcaggc gcgcaggaag aagatgtgct gtttagctgg
    21121 cggatggtgg ggcattatcg ttattttgtt aaataagcga tcgccccaca tgaagaaaaa
    21181 attcagatgc tttttacact gacagcttct tttttcttac ggctcaccag cgccagtgcc
    21241 agcacaatca gcacaatacc gctaccttca acggcccccg gattctcacc gagcagccac
    21301 caggaaaaga gtacgccgca aacgggaacg gccagcgtgc ttaagctggc aatactggca
    21361 ggcaagtttt tcaatacaaa caaccataag ctccacgcca gcgccgtcgc cagaatcgca
    21421 ctgtaggcca gcgcccagaa cacggtgggc tgccagtcaa tttcacgttg cggcaccagt
    21481 aaagcgacca cactcatcac cagcgccgca tagagcatct gccaggatgt taacgataat
    21541 aaatccacgc gcggatgacg ggtgtatagg cgtttagcaa caatcgcgct cgccccccaa
    21601 ctgacgccgg agaggattgc cagcatggca cttttcattg aagagaaatc gagttgccac
    21661 ggctgcaaca ccaaaaataa gccgaaagcg gcaatcagta tcgcgaaata ttgcccacgt
    21721 cgcaggcgtt caccgagaaa caacgcggcg aaaatcacca cccagaacgg catggtatag
    21781 cttaggatcg ccaccttccc cgctccgccg ctgaccagcg cccactgcgc caggccaacc
    21841 atcccgcagg tttgtaacag ggcaatggct aaggtgtatt taaacggtgt cgggcgcatt
    21901 ccgcgaccac gtaataaaag gacgatgaat aaaacgagag cgccgaaaat gcagcgtaag
    21961 gcggtaaagt cgaaggcacc gatgtagctg gtgacttgct tcatgaaaat ccagctataa
    22021 ctccagataa gtgtgagcac aaccaggcca ctgattgcca gtgggttgct ctttcctgcg
    22081 gcagacatga gagatccagt gtgtagtttt cctgtggaca ctatacgccg gtaaagaaac
    22141 ttcagcgact gcctgaaaga cagtcgctga agaaggtcag attacagcag gtcgaaacgg
    22201 tcgaggttca tcactttcac ccatgccgcc acgaagtctt taacaaactt ctcgtgggca
    22261 tcgctgctgg cgtaaacttc cgctaccgca cgcaggacag agttagaacc aaataccaga
    22321 tccgcacggc tggctgtgta tttcacttcg ccggtttcac ggtcacgacc ttcgaacagc
    22381 tctttcgatt cgtcagtcgc tttccactcg taacgcatat ccagcaagtt cacgaagaag
    22441 tcattgctca atacgccaac gcggtcagtg aagacgccgt ttttgctgcc atcgaagttg
    22501 gcacccagta cacgcatccc gcccaccagc gcagtcattt ccggcgcggt cagcgtcagt
    22561 tgctgtgctt tgtcgatcag cagtgactcg gtggtggaaa catccagacg agcgcgatag
    22621 ttacggaaac cgtcagcaat tggctccagc agctcgaaca tctcaatgtc agtctgatcc
    22681 tgacgcgcat caacgcggcc cggcgcaaac ggtacatgaa tgctcaaacc tgcggcgctt
    22741 gcggctttct caacaccaac cacaccagcc agcacgatga tatccgccag cgaggcttta
    22801 ccagactctt tctggatttt ctccagaaca ggcagagcac gaacggctgc ggcgttcaca
    22861 tcccagtcgc gctgcggcat taatgccaga cgcgcaccgt tggcaccacc gcgtttgtcg
    22921 ccaccacgga aggtagaagc agaagcccag gccaccgata ccagctcact aacagacaga
    22981 ccagaatccg caatcgcgaa tttcagatcg ataatgtcct gctcggtcgg gttgtagatc
    23041 ggctgcggca gcggatcttg ccagatcaga tcttctttcg gcacttccgg cccgatgtag
    23101 cgagatttcg gccccatatc cctgtgcgtc agtttgaacc aggcacgggc aaaggcttcg
    23161 ttgaacgcct gcggatcgtt gaggaaacga cgagagatct tctcgaactc aggatcaaaa
    23221 cgcagcgtca ggtcggtcac cagcattgtc ggtttacgtt tcttcgacgg atcgaacgga
    23281 tccgggataa tttccggtgc gtctaccgct tcgaactgga ttgctccagc cggactgcgg
    23341 gtctgtaccc actcatactt gaacagattc tcgaagaaat agttgctcca ctgggtcggc
    23401 gtctgggtcc agactacttc cagaccagag gtaatggcat ctgcgccaac gccgctgccg
    23461 taagtgctcg cccagcctaa accttgttct tcaatcggtg cagcttctgg atcaggacct
    23521 acgtttgatg tcggaccggc accgtgggtt ttacccagcg tatgaccacc cgcaatcagc
    23581 gccacggttt cttcgtcgtt catgcccatg ttgccgaagg tcgcgcggat agctgctgcc
    23641 gcagaaagcg gttcgccgct gtgatccggg ccttccgggt taacgtaaat caggcccatc
    23701 tcggttgcac ccagcggtgc tttcgccagc gcttccggat gacggtgagt cagccaggct
    23761 ttttcatcac cccagttaac atccagatcc ggttcccaga cgtcttcacg accggcacca
    23821 aaaccgaagg tacggaagcc ggagttttcc agcgccacgt tacctgcgag gataaacagg
    23881 tcggcccagg agattttctg accatatttc tgtttgattg gccacaacag gcgacgcgct
    23941 ttatcgaggc ttacgttatc cggccaggag ttcagcggtg caaaacgttg ctgaccacga
    24001 cccgcgccgc cgcgtccatc gattgaacgg taagtacctg cgccgtgcca ggccatacga
    24061 ataaacagac cggcgtaact gccccagtcg gcaggccacc acggctgaga ttctgtcagc
    24121 agggctttca gatctttttt caggccgtag taatctaatt tgctgaattc tttgcggtag
    24181 tcaaagtcct cacccagtgg gttagaacga ttagaatgtt ggtttaacag gtcaacacgg
    24241 agttgatttg gccaccagtc gcgagtggtt gtgcccgccc ccgcgctctg gtcgtgaccg
    24301 ccctgatgga acgggcattt gccagtggct gtggtgttat gaatatcgtc tgacgtgctc
    24361 atcaatgtgc tcccctctac agtgttaccg ttacgataca cagcgttaga gagaagttat
    24421 aattgaatta atccacggat gcgatagttg agatcttaca tattgttggt taaagagatg
    24481 tagatcaaat tgatcgtaat taagcttttt tgattatgtc cgaattcgga caaatgcttt
    24541 ataaaaaagg gttttaggtt catatccttc aagtaatgag ggagaaaaca aggctgataa
    24601 ccttattttc ccgccctcat ttcgaggcag cattttgtgc tctgtttaaa atttgtgatc
    24661 actgtgtgat tttcacaaaa gccacactat ttataaacca ggtcgaacac ccagcgtatg
    24721 gcaaatcgcg taactcattt cagcgcggtt aagcgtatag aagtggaaat ctttcactcc
    24781 ttcacggctt aaaatcttca ccatatccat ggcaatattc gctcccacca gtttgcgggt
    24841 ttcggcatcg tcgtccagcc cgtcaaacat ttgtgccatc cacgccggaa tacgcacgtt
    24901 ggtcatatcg gcaaatttct tcgcttgttt aaagttcgag accggcaaaa ttcccggaat
    24961 aatttccaca tcaatacccg ccgatacgca gcggtcacga aaacgcaggt agctttcgac
    25021 atcaaagaag aactgagtaa tcgcgcggtt ggctccggca tcgactttgc gtttcaggtt
    25081 gagcaaatcc gcctgagcgc tttttgcttc cgggtgaacc tccggatacg ccgccacgga
    25141 gatatcgaaa tctgccactt cttttaacag cgtcaccagg tcagaagcat acatttccgg
    25201 cttaccactt cccggcggca gatcgccacg cagcgccacg atatgacgaa taccgttatt
    25261 ccagtagtcg cgtgcaatgg tgcgcagctc gtcgggcgtc gcatcaatgc aagtaagatg
    25321 cggtgccgct tccagaccag tgcgatcttt aatgccttta ataatgctgt gcgtacggtc
    25381 gcgctcgccg gagttcgcgc cataggtcac cgatacaaac ttcggtttca ggctgctaag
    25441 gcgatcgatg gagttccaca gggtctgctc catttcactg gtacgcggcg ggaaaaactc
    25501 gaacgaaacg ttaatctgcc cctggacttc tgccaggctc tgattcaggg catcccgctg
    25561 gctggcgtga aaaaagctca taccttacct catcaatcgc ttgtagttat atgttgtgtt
    25621 gtgcacatct atccgtttag acgtccagat gtaaagatga aggaaaagcc gaagggcgtc
    25681 aaccgaaaaa tgaccaatga tggtgaggaa aaatcaggga agatgaaaaa acttcaggat
    25741 ggtaagaaaa agaaaatccg gtatgcaaag caaaccggac tgcaaatata aactaaccat
    25801 gtacttattg cttcttgccg gattcgtcgt aaacgcttaa tccggcctaa tcctatgata
    25861 attcaatata tttcattaga aaccataaac ccgaaaacat gagtaccggg cattattaaa
    25921 tttctgaaat tacaacaact gtgccagccg gttgatatcc gactgaatcg ccccggcggt
    25981 gacgtcgcgc cccgcgccag gcccacggat caccagcggg ttgtcgcgat accagcggct
    26041 ttcgatggca aagacgttat cgcacggcag cagtgatgcc agcggatggt cttcacgcac
    26101 cgcctccacg cccacacgcg ctttgccgtt ggcatcgaaa cgcgccacgt agcgcagcac
    26161 cagccccatt tcgcgggccg cttccagccg ttgcaccatc tgctcgttaa gttcatcgcc
    26221 attttcaaag aaatggtcga tgctgccgcc ttcgcaatgg gcaggcacca gcgactccac
    26281 gcgtacctga tccggttcga tgttgtaacc tgcttcacgc gccagaatca ccagcttgcg
    26341 cattacgtct ttgccggaaa ggtcatcacg cgggtcaggc tcggttaacc cttgctgcca
    26401 cgcctgatcc accagctcgg taaacggcac gctaccgtcg aattgcagga acagccagga
    26461 gagcgtgccg gagaagatcc cactgatcga caaaatagta tcgccgctgt cgatcagatc
    26521 gcgcacggta tggttgatcg gcaagcccgc tccgacggta gcattgtaca gccagtgacg
    26581 cccggttttt tcgaaggcgt cgtggatctg gcgatattta ttgctgtcgc tggctcccgc
    26641 cagtttgttg gcgctgatga cgtggaaacc gtggctggcg aaatcaagat actgatcagc
    26701 aagctgctgg ctggcggtaa cgtccagcac cactaaatca tcatacggat gggcgcgcat
    26761 ccacaggaac aacgactctt catcctgctc aaccgcttca tcgttgaaga aggctaacgc
    26821 gcggctggcg tccagcccgt catagctcaa caggctgcgg cggctgtcca ccacacctgc
    26881 cagcacaaac tcaaagccgg tacgtgccga aagcgtgctc tgctcacggg cgaacagttc
    26941 cagccaacgg gaaccgatat tgcccttacc gaacaatacc aggccgatgc gtttttctgc
    27001 gcggaagacg gactgatgca gcccctgaat caggctttcg gtcgggccgg tgcgcagtac
    27061 tgccaccagg ctgatgccgt catcggactg ccaggtaaat tcgaccggct ggcctttcag
    27121 ttgctgccag aagcggtggc aatgcagcgg gttacgggtg acgcctgcac cgaccatcgc
    27181 caccagcgcc agcccctgac gcaggcgcag ttcgccaggt aatcccgctt cgtcgaggat
    27241 tttcagcgca ctgtcggcca cttctgaggt gtagcaaaat tgcagcaact ggcgatcgtt
    27301 atgtacgcca accgccagcg ggcgtacctg cgcgcgtttc aggatttggt cgatctcttt
    27361 atgcgccagt ttgaaatcct gactggcggg cacctgaaac tcaatcaaac agacatcatc
    27421 gtggctggtg acaatacgcg caccagtacc ggaggccagc acgcgttcaa tgcgcgtgga
    27481 accttgatcc ggcgtgtagc tacagcgcag ttgcaggtcg atttcgctgc cagaaaccgg
    27541 ctgtaaagta cgggcgtgaa gaacgggagc cgccaggcgc gccagttcgc tggcctcatc
    27601 cagacgcagc aacggcagca ggcaggcatc tttcacttta cgcgggtcgg cactgtatac
    27661 cccggcgacg tcgctccaga tggttacgcg agaaacaccc gccagcgcac cgatttgtgt
    27721 cgcggaatag tcggaaccgt tacgccccag cagcaccgtt tcaccggcgt tgttgcggct
    27781 gataaatccg gtcaccacca gacgtttgcc cggatgttgc accagcagct gttgcagcaa
    27841 cgggtaagaa agcccttcat caacctgcgg ttgtgcggcg cgttcagcgc gtaaaaactc
    27901 gcgggcatca agccaggccg ctggcagccc ttgttgatta agtaccgcag acatcaggcg
    27961 tgccgaccac acttcaccgt gccccaccac ttccgcatac actgcgtcgt taataccgct
    28021 gtcgagcagc gccgccaggc gctcaaggtc gctgacaaaa gcgctaatga ggctatcggc
    28081 ttcttcagcg ggtagcagac cgctaatcag atcgcactga tagcgacgca gcgtttgttg
    28141 aacctgatgc gcagagagac gatcggtctg gcttagtttc aaccagttaa tcaactggtt
    28201 agtggtgcta ccggcggcgg aaaccaccat catatcgtca ggctgagagt actccgccat
    28261 aatgcccgcg acacgcaaat aacacttcac atcagccaga ctactgccac caaatttatg
    28321 cagctgacga cctttcgccc ctgcctgcgc aatcacactc atttttaccc cttgtttgca
    28381 gcccggaagc cattttccag gtcggcaatt aaatcttcgc catcttcaat accggtggag
    28441 atacgcagca gcgtctcgga gatcccggcg gcagcacgcg cttctggtgc catgcctgca
    28501 tgtgtcatgg ttgcggcgtg agagattaaa ctttccactc cccctaatga ttccgccagc
    28561 gtaaacaacg acagtccacc caggaaacga cgcagcgtct gctcatcgcc atccagttca
    28621 aaactcaaca ttgcgccata gcctttttgc tggcgcgcgg caatttcatg cccctgattt
    28681 tccggcagcg acgggtgata cagttttttc accaacggct gggtttgcag atatttcaca
    28741 atcgcctgcg cgttacgctg ggccaactcc atgcgcggca ccagcgttcg caacccgcgt
    28801 agcagcagat agctgtcaaa cgcgccgccc gtcacgccaa tattgtttgc ccaccaggca
    28861 agttcagtga caacgtccgg gtctttagca atcaccacgc cggccaccac gtctgagtga
    28921 ccgttcagat atttcgtgca tgaatgcaac accagatcgg cacctaatgc cagcggattt
    28981 tgtaatgccg ggcttaagaa ggtgttatcc accacgctca ccgccccgac ttcccttgcc
    29041 agatggcaga ttttcgcaat atccacgacg cgtaacaatg gattacttgg gctttctacc
    29101 agtaccagtt tgggtttatc tgccagcgct gcccgtaatg cctgttcatc gccttgatca
    29161 acaaacaaca cgcgatagca accgcgtttc gccaggctgt cgaacaggcg atagctaccg
    29221 ccgtagcagt cgtgcggcgc taccagcaga tcgccaggtt tcaaaaagac ggtcgttacc
    29281 aggtgaatcg cggacatgcc ggtattagta agtactgcac cagcaccgcc ttccagttct
    29341 gccagtgctc gctgaaccac atcgcgcgtt gggttgccac gacgcgagta atcatgcgcg
    29401 cgcggttcat taaatccggt aaagttatag gtactggaaa gatggatggg tgggacaacg
    29461 caaccatact gttcgtcgtc atttaacccg ctacgcactg cgatggtggc ctgtttacgc
    29521 gtcatgtgat gatgttccct gggctttatt ggtgaaatgt caggcaccag agtaaacatt
    29581 gtggtaatgg acgtcaatac atctggacat ctaaacttct ttgcgtatag attgagcaaa
    29641 tcccaaatag ccgttaaaat tatatgcatt atcacgccag caggtggatt acccgatgtc
    29701 acggtagcgc ctgtacggta aactatgcgg gtttacggcc agtgcccaca ttaactgtgt
    29761 ggtctggtct caatttattg acgaagagga ttaagtatct catggctgaa tggagcggcg
    29821 aatatatcag cccatacgct gagcacggca agaagagtga acaagtcaaa aagattacgg
    29881 tttccattcc tcttaaggtg ttaaaaatcc tcaccgatga acgcacgcgt cgtcaggtga
    29941 acaacctgcg tcacgctacc aacagcgagc tgctgtgcga agcgtttctg catgccttta
    30001 ccgggcaacc tttgccggat gatgccgatc tgcgtaaaga gcgcagcgac gaaatcccgg
    30061 aagcggcaaa agagatcatg cgtgagatgg ggattaaccc ggagacgtgg gaatactaaa
    30121 gcggaaaaga gcggcgcgga gtggaaccgc ctgatgcgct acgcttatca ggcctacgtc
    30181 atattgcaat ttattgaatt tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat
    30241 ccggcataaa caacgagcac gttgtctgcg acccaccgct ttttatacat ggacgtttaa
    30301 ctatgaaaaa caggctgctg atcctcagcc tgctggtttc tgtacctgcc tttgcctggc
    30361 agccgcaaac tggcgacatc atctttcaaa tctctcgctc atcgcaaagt aaagcgatcc
    30421 aactggcaac ccattccgat tacagccaca ccggtatgct ggtgatacgc aacaaaaagc
    30481 cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag tggatcgccc
    30541 atggtgaaaa gggcaaatac gtcgttcgcc gcgttgaagg cggactgagt gttgaacaac
    30601 agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa accgtatgat ttcagcttct
    30661 catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc
    30721 tgggaatgcg cgtgggcgaa cagcagaagt tgaaagagtt tgatctcagc aacccgctgg
    30781 tccaggcgaa gctcaaagaa cgctacggta aaaatattcc gcttgaggaa acggtagttt
    30841 caccacaagc cgttttcgac gcgccacaac tcaccacggt tgccaaagag tggccgctgt
    30901 tttcgtggta atggatgatg aaaaaaataa ttcccgccgt agtctgtttg ttgctttgta
    30961 gttgcacgac ctttccgcaa gctgtcgctc cggtaaacgg tgatgcctcc aattagttga
    31021 attaaggata taatactcgc ttttaaggtt gtttcatggt cacagttaac cttcattgcc
    31081 cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaaaga catgatcgat
    31141 tccgcggccg tgactgtcac cgggtatttc agctcagtta ctgttacgag ccccgtaaac
    31201 cgggagttaa agaccaaatc gctgaaatga ctttcaacgg tgccggggtt cgcgatactg
    31261 cccgtacgct gaaaatcggc attaatacag tcatccgaac tttaaaaaac tctcgccacg
    31321 aagaataacg tcatccccgg tcgctcatgc tgatgtggca ctcatctgca aacttagtca
    31381 gcaatggagc tttgtcggca gtaaagcgcg acagcactgg ctctggtacg tgtacaacac
    31441 gaaaactggt ggtgttctgg cctacacttt tggcccccgg actgacgaaa cctgtcgtga
    31501 attgcgggcg ctgcttaccc ttttaccatc ggcatgttga ccactgacga atggggtagt
    31561 tacgctagag agttaccgaa ggagaagcat ctgactggta agatttttac tcagcacatt
    31621 gagcgtaaca atctgaccct gagaacccga atcaaacgtc tggcccgcag aaccatctgt
    31681 ttctcgcgtt ctgtagaact tcatgaaaaa gtcatcggtg cttttatcga aaatatatgt
    31741 tctactaatt ggagtcatca cccttctgat aagctttcct tggatgatta ccagactgaa
    31801 tacacagcca aagattttta cctcattctg ctcttttttc tcaaatgaca tatattatat
    31861 tttacatcat attcataggc atatgac
//
LOCUS       DAZFWP020000044        31674 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFWP020000044 DAZFWP020000000
VERSION     DAZFWP020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.044"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            152..1534
                     /locus_tag="ACHK0Z_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             152..1534
                     /locus_tag="ACHK0Z_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU4501897.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1544..3862
                     /gene="yicI"
                     /locus_tag="ACHK0Z_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     CDS             1544..3862
                     /gene="yicI"
                     /locus_tag="ACHK0Z_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU4501898.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRQDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(3915..5624)
                     /locus_tag="ACHK0Z_RS09345"
                     /old_locus_tag="pgaptmp_001869"
     CDS             complement(3915..5624)
                     /locus_tag="ACHK0Z_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4501899.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(5745..7136)
                     /gene="xanP"
                     /locus_tag="ACHK0Z_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     CDS             complement(5745..7136)
                     /gene="xanP"
                     /locus_tag="ACHK0Z_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU4501900.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7416..8621
                     /gene="gltS"
                     /locus_tag="ACHK0Z_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             7416..8621
                     /gene="gltS"
                     /locus_tag="ACHK0Z_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4501901.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            8624..9490
                     /locus_tag="ACHK0Z_RS09360"
                     /old_locus_tag="pgaptmp_001872"
     CDS             8624..9490
                     /locus_tag="ACHK0Z_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501902.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(9475..11556)
                     /gene="recG"
                     /locus_tag="ACHK0Z_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             complement(9475..11556)
                     /gene="recG"
                     /locus_tag="ACHK0Z_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU4501903.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11562..12251)
                     /gene="trmH"
                     /locus_tag="ACHK0Z_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             complement(11562..12251)
                     /gene="trmH"
                     /locus_tag="ACHK0Z_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU4501904.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12258..14366)
                     /gene="spoT"
                     /locus_tag="ACHK0Z_RS09375"
                     /old_locus_tag="pgaptmp_001875"
     CDS             complement(12258..14366)
                     /gene="spoT"
                     /locus_tag="ACHK0Z_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU4501905.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14385..14660)
                     /gene="rpoZ"
                     /locus_tag="ACHK0Z_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             complement(14385..14660)
                     /gene="rpoZ"
                     /locus_tag="ACHK0Z_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU4501906.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14715..15338)
                     /gene="gmk"
                     /locus_tag="ACHK0Z_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             complement(14715..15338)
                     /gene="gmk"
                     /locus_tag="ACHK0Z_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU4501907.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15596..17278
                     /gene="ligB"
                     /locus_tag="ACHK0Z_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     CDS             15596..17278
                     /gene="ligB"
                     /locus_tag="ACHK0Z_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU4501908.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVTEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17275..17892)
                     /locus_tag="ACHK0Z_RS09395"
                     /old_locus_tag="pgaptmp_001879"
     CDS             complement(17275..17892)
                     /locus_tag="ACHK0Z_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU4501909.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18184..19008)
                     /gene="dinD"
                     /locus_tag="ACHK0Z_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /pseudo
     CDS             complement(18184..19008)
                     /gene="dinD"
                     /locus_tag="ACHK0Z_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            complement(19229..20092)
                     /locus_tag="ACHK0Z_RS09405"
                     /old_locus_tag="pgaptmp_001881"
     CDS             complement(19229..20092)
                     /locus_tag="ACHK0Z_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU4501910.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20219..20935
                     /gene="rph"
                     /locus_tag="ACHK0Z_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             20219..20935
                     /gene="rph"
                     /locus_tag="ACHK0Z_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU4501911.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21001..21642
                     /gene="pyrE"
                     /locus_tag="ACHK0Z_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             21001..21642
                     /gene="pyrE"
                     /locus_tag="ACHK0Z_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU4501912.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(21679..22275)
                     /gene="slmA"
                     /locus_tag="ACHK0Z_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             complement(21679..22275)
                     /gene="slmA"
                     /locus_tag="ACHK0Z_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU4501913.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22382..22840)
                     /gene="dut"
                     /locus_tag="ACHK0Z_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             complement(22382..22840)
                     /gene="dut"
                     /locus_tag="ACHK0Z_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4501914.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRVCLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(22818..24038)
                     /gene="coaBC"
                     /locus_tag="ACHK0Z_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             complement(22818..24038)
                     /gene="coaBC"
                     /locus_tag="ACHK0Z_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU4501915.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24210..24878
                     /gene="radC"
                     /locus_tag="ACHK0Z_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             24210..24878
                     /gene="radC"
                     /locus_tag="ACHK0Z_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4501916.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25095..25331
                     /gene="rpmB"
                     /locus_tag="ACHK0Z_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             25095..25331
                     /gene="rpmB"
                     /locus_tag="ACHK0Z_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU4501917.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25352..25519
                     /gene="rpmG"
                     /locus_tag="ACHK0Z_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             25352..25519
                     /gene="rpmG"
                     /locus_tag="ACHK0Z_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU4501918.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            25617..26426
                     /gene="mutM"
                     /locus_tag="ACHK0Z_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             25617..26426
                     /gene="mutM"
                     /locus_tag="ACHK0Z_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU4501919.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVELVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26465..26944)
                     /gene="coaD"
                     /locus_tag="ACHK0Z_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             complement(26465..26944)
                     /gene="coaD"
                     /locus_tag="ACHK0Z_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU4501920.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(26952..28229)
                     /gene="waaA"
                     /locus_tag="ACHK0Z_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             complement(26952..28229)
                     /gene="waaA"
                     /locus_tag="ACHK0Z_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU4501921.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            28642..29700
                     /gene="rfaQ"
                     /locus_tag="ACHK0Z_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             28642..29700
                     /gene="rfaQ"
                     /locus_tag="ACHK0Z_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU4501922.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29697..30821
                     /locus_tag="ACHK0Z_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             29697..30821
                     /locus_tag="ACHK0Z_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4501923.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            30814..31611
                     /gene="rfaP"
                     /locus_tag="ACHK0Z_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             30814..31611
                     /gene="rfaP"
                     /locus_tag="ACHK0Z_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU4501924.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7458 a         7872 c         8516 g         7828 t
ORIGIN      
        1 caatgatata tgagcgaaat taacgctacc acgatcacat aaaacaagaa tcataaatta
       61 ataaccagat atcggaatat tcgctctcac agggatggtt tacaaaatgc gttatcggtc
      121 tcagcacccc tatagcatca aggaaaagca gatgaagagt gaagtgttgt ccgttaaaga
      181 gaaaattggt tatggcatgg gagacgccgc cagccacatt attttcgata acgtaatgtt
      241 atatatgatg ttcttttata ccgatatttt tggcattcct gccggttttg tcggaaccat
      301 gtttttggtc gctcgtgcac tggatgcgat ttccgatcct tgcatggggt tgttggccga
      361 tcgaacgcgc tctcgctggg gtaaatttcg tccgtgggta ctgtttggcg cactgccatt
      421 cgggatcgtc tgtgtactgg cctatagcac gccagatctc agtatgaacg gcaaaatgat
      481 ctatgcagca attacttaca ccctacttac cttactttat accgtcgtca atatccctta
      541 ctgcgcattg ggtggtgtaa tcaccaatga cccgactcag cgtatctcgc tgcaatcctg
      601 gcgttttgtg ctggcgaccg cgggaggcat gctttctact gttctgatga tgccactggt
      661 taatttaatt ggcggtgata ataaaccact cggtttccag ggcggtatcg cggtcctttc
      721 cgtggtggca ttcatgatgc tggcattttg tttcttcacc actaaagaac gcgttgaagc
      781 accacctaca acaacgtcta tgcgggaaga tttacgtgat atctggcaaa acgaccagtg
      841 gcggattgtc ggtttactaa ccattttcaa tatcctggcg gtgtgcgtac gcggtggggc
      901 gatgatgtat tacgtcacat ggattttggg cacgccggaa gtgtttgtcg cttttctcac
      961 cacttattgc gtgggtaacc tgattggttc cgcactggca aaacctctga ccgactggaa
     1021 atgtaaagtc actatcttct ggtggacgaa cgccctgctg gcagtgatta gcctcgcgat
     1081 gttctttgtt cccatgcagg ccagcatcac tatgtttgtc ttcatcttcg tgattggtgt
     1141 gttgcatcaa ctggtgacac ctatccagtg ggtaatgatg tccgataccg tcgactacgg
     1201 cgagtggtgc aatggtaaac gcctgaccgg gatcagtttt gctggcacgc tgtttgtgct
     1261 caaactgggg ttggccttcg gcggcgctct tatcggctgg atgctggctt atggcggata
     1321 tgatgcggca gaaaaagcgc agaacagcgc cacgattagc atcattattg cgctattcac
     1381 gattgttccg gcgatctgtt atttgctgag cgcgattatc gctaaacgct actactcact
     1441 cacgacgcac aatctgaaaa ccgttatgga acagctggct cagggtaaac gccgttgcca
     1501 gcaacaattc acctctcaag aagtgcagaa ctaaggaacg gcaatgaaaa ttagcgatgg
     1561 aaactggttg attcaacctg gcctcaattt gattcacccg cttcaggtgt tcgaggttga
     1621 acagcaggat aatgaaatgg tggtctatgc tgccccccgt gatgtgcgtg aacgtacctg
     1681 gcagcttgat acgcctttat ttacgttgcg ctttttctcc ccacaggaag gtattgtcgg
     1741 tgtgcggatt gagcattttc agggggcgct gaataacggt cctcattatc cgctcaatat
     1801 tttgcaggac gtgaaggtca caatcgaaaa cacagaacgt tatgctgagt ttaaaagtgg
     1861 caacttaagc gcgcgtgtca gcaaaggtga gttctggtca ctggattttc tgcgcaacgg
     1921 cgaacgtatt accggtagtc aggtgaaaaa taatggctac gtgcaggaca cgaataatca
     1981 acgcaattat atgtttgagc ggcttgatct tggcgttggc gaaacagttt acggtctggg
     2041 agagcgcttt actgccctgg tgcgcaatgg ccagacggta gagacctgga accgggacgg
     2101 cggcacaagt actgaacagg cgtataaaaa tatcccgttc tacatgacta accgtggtta
     2161 tggggtactg gtcaatcatc cccagtgtgt ctcttttgaa gtgggatcgg agaaagtctc
     2221 caaagtgcag ttcagcgttg agagtgaata tctcgaatac tttgttatcg acggcccgac
     2281 gccgaaagcg gtacttgatc gttatacccg ctttactggt cgtccggcgc tgccgcccgc
     2341 gtggtccttc ggcctgtggc taaccacttc atttaccacc aactacgacg aagcgacggt
     2401 aaacagcttt atcgatggta tggcggaacg caatctgccg ctgcatgttt tccactttga
     2461 ctgtttctgg atgaaagcct tccagtggtg cgattttgag tgggacccgc tgactttccc
     2521 tgacccggaa gggatgatcc gccgcctgaa agcgaaagga ctgaaaatct gcgtctggat
     2581 taacccctat atcggtcaaa aatcccccgt ctttaaagag ttacaagaga aaggctattt
     2641 actcaaacgc caggacggtt cgctatggca gtgggataaa tggcagccag gtctggcgat
     2701 ttatgacttt accaatccgg atgcctgcaa atggtacgcc gacaaactga aaggtctggt
     2761 cgcgatgggc gttgattgct ttaagaccga ctttggcgaa cgtatcccaa ctgatgttca
     2821 gtggtttgac ggttccgatc cgcagaaaat gcataaccat tatgcgtaca tctacaacga
     2881 actggtgtgg aacgtgctca aggacaccgt tggtgaggaa gaagctgtct tgtttgcccg
     2941 ctcggcctcc gtcggtgcgc agaaattccc ggtacactgg ggtggcgatt gttacgctaa
     3001 ctacgaatca atggcggaaa gcctgcgcgg tggtttgtct attggccttt caggttttgg
     3061 cttctggagc cacgatatcg gcggctttga aaataccgct ccggcgcacg tttacaaacg
     3121 ctggtgcgcg tttggtttgc tctccagcca tagccgttta cacggtagca aatcttatcg
     3181 tgtgccgtgg gcctacgatg atgagtcctg tgatgtggtg cgcttcttca cgcaactgaa
     3241 atgccgcatg atgccgtatc tgtatcgtga agctgcgcgt gcgaacgcgc ggggtacgcc
     3301 gatgatgcgg gccatgatga tggagttccc ggacgatccg gcttgtgatt accttgaccg
     3361 tcaatacatg ttaggcgaca acgtgatggt tgcgccggtg ttcactgaag cgggcgatgt
     3421 gcagttctac ctgccggaag gtcgctggac acacctgtgg cacaacgatg aactcgacgg
     3481 tagtcgctgg cataaacagc agcacggctt cctgagtctg cccgtttatg tgcgtgataa
     3541 cactctactg gcgctgggca acaacgatca acgtcccgat tacgtgtggc acgaaggcac
     3601 ggcattccac ctctttaatc tgcaagacgg gcatgaagcc gtctgtgaag tgcccgctac
     3661 tgacggatcg gtgatcttta ctttaaaagc agcacgtact ggcaacacga ttactgtgac
     3721 tggtgcgggc gaggcgaaga actggacgct gtgcctgcgc aatgttgtga aagtaaatgg
     3781 tctgcaagac ggttcgcagg ctgaaagtga gcaggggctg gtggtgaagc ctcaagggaa
     3841 tgcgctgaca attacgttgt aatctattga tggaatgccg ggtacgtctg agtcgtaccc
     3901 ggcgagattg atgtttaccg caccggctcc gtcgatgtaa cttcaccgct ggaaacgatg
     3961 ccagcattca tggtttgagt cacttgctgc tttgcggcgt tcacggcatg caattgcccg
     4021 ctaaccgtag gtttcaacgg gacattggcc tgaatatcgc cactggcggt aagctgaata
     4081 ttgccgtcgc cagtcaacgg taatttaggc catccccact gttgcaaaat attcaccggc
     4141 acaccgcgtc cattcaggct gatatgtgtc tgacgttgtg gcgtttgtga aacactggcg
     4201 gtggcttcca gaatgccttt ttcagtaaat gcactcagtt cgctgatatt caccgtgctg
     4261 ctgttggcgg tcagcgccag cgacgggcga cgaacatcaa cacgattgaa tgtcgcggcg
     4321 gcggcattca gattcgccga gccactccag acgccccatt tatgatcggt aaccagcgtc
     4381 aggttggcac cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg gtcgatatca
     4441 atgatcagat tgcggctggc gctaaatctc ttcagttgca ggctgtttaa ccaaccgggt
     4501 gtcgtttcca tccacaactg ttgccagttt ttcggcaagg tatattccag cccggcaatt
     4561 gccgcatcat caaggatcaa cgttttcccg tcacgcagcc agttccctga cgttctgacc
     4621 ataccccctt cccagcggct ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc
     4681 gcgtttataa tcgggtcaaa taaatgcagc gaaccataaa tgaactcgct ggcgttcatc
     4741 gacagtttgc cttcttgtgt ctgccagtca tctttactga aggtcatgtt gcgcaagctg
     4801 agatcgaggt cggtcaccgc ccagtccgga ccttgcaaac gagcatcgat cacttccagg
     4861 cgaccaatct gcaacgacgg gacagagcgt aatggcgcaa agaagtcggt tagcgatttt
     4921 tcgctttgta gacggatatc cgccatgcgc agattttcca cttgccagct gccgtcggcg
     4981 ttacgctgcg cgtttccggt taatgtcccg cgggcgatgt cggcacccag gttagtcagc
     5041 gtaacgcgat cgttatcaat actgccttca atcagtacat tggtggcagg aacatcattg
     5101 agcgaaagcg atccggcact aaactgaatc tgcgccttcg tacccagcac tttaccggct
     5161 tctggtgacc acggaaccac gccgccattt acccgctgcg cgctcagttt ccattcgcta
     5221 ttcgggctat taaacgccat atcacgcagt tgcagacgat cggctttgaa cggtagcggc
     5281 gcggtctggt cggtgagatt cagcgtcccg ttttccagca ggatggtatc gacatggcgt
     5341 ggttcggtca gttgccgact gcttagcgca atgtcgacac tttttgccac cagggtcgcg
     5401 ggctgaccat cacgaccaaa cgtgacgttc tccagcacga tatgagatgg cgcggaaaaa
     5461 cggtgatcca tcgccccgaa ggccagatga tagtcgctat tctcggaaac ccatgcgctg
     5521 atatgttctg ctccccagcg ggtttgcaga agaaaataga ggccagcgat caccactaac
     5581 agagcgatga gaatgtagag aagcagcttc ccaataaatt tcatggtctt ccatcccgtg
     5641 aaatgcacat aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa
     5701 agaggtgaga ctgcggcggt aattatctta ccgccgccag tgaattactg tttttctggt
     5761 gggaaaatca gattcagcac gatggcagta ataccgcccg cggcgatccc ggaggagagc
     5821 aggtttttca gccattcagg ggcaaactgc aaaatcagcg gctgctgaga cacgcccaga
     5881 ccaaccgcca gcgacagcgc gataatcaga atcgcccgac ggttcagcgg ctcacgagaa
     5941 acgatacgca caccggaggc ggcgatggtg ccaaacatta caagcgttgc gccgcccaga
     6001 accggttctg gaatgtgttg tacaaaaccg ctcactgccg ggaacagacc cagcacgatc
     6061 aacatcagcg cgacgacaaa accgacatag cggctggcaa caccagtcaa ctggatcact
     6121 ccgttgtttt gcccgaagca ggagttcggg aaggtgttaa acaccgccga aacaaacgag
     6181 ttcaggccgt ttgccagcac gccgcctttc aggcgtttca tgtacagcgg accggacact
     6241 ggctgttcgg aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc
     6301 ataaagacca gcatcagcgg cagcagcaga ctccattcaa tgccaagacc gtaatagagc
     6361 ggcgttggca ccataatcag ttcttgcgtc atcggttcgt tgctttctgg caacatgccc
     6421 ataaaccacg ccagcgcata tccggccgcc atcgcaatca ccagtgaggc cacgcgtaag
     6481 taagggttac gttgacggtt aagcaggata attaaggcta agaccacgcc tgccagcagc
     6541 agatttttcg gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc gccaatggac
     6601 gttaacccaa cctgaattag cgacaggccg ataatcatca ccacaacgcc agaaaccagc
     6661 ggcgtaataa tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc ggtgcaactt
     6721 gccagcatca acgtgccgaa caaagccgcc atcatggtag gaacatcagc accaccggtt
     6781 ttcagcgcgg taccgcccat aatcagcggg gcaacaaagt tgaagctggt gccctgaata
     6841 gacaacagcc cggagccaac cggaccccag gccttaattt gaataatcga tgccacaccg
     6901 gaggcaaaca gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc
     6961 tggcagatta atagcgctgg cgtgatcacc gcaacgaaca tcgccagcag atgctgacag
     7021 gcggcaaaca gggtttgagg aagcggcgga cgatcttcaa gacggtaaat cagttcgctg
     7081 ttttgagtct gcgcaaccgg ttgcgcattt tctgactcga gggtggaaac agacatcgaa
     7141 aacaatcccg tggtagcaaa gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt
     7201 gccacatcct catctaaata tttaaatgag ataaatgcac ttttttatat aacttttggt
     7261 atttttctgc ttaaaatcca tgccatttgt tcattaacag ctgagaattt gacccaggaa
     7321 agcataactc gatacccgtc atagttcatg aagttgccac tgcgcgatga agtatgacga
     7381 gtatgaaaga gtgatgcgga tacaaaggag taactatgtt tcatctcgat actttagcaa
     7441 cgcttgttgc cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat tccgtctcct
     7501 ttttgaagaa atacaccata ccggaacctg ttgcgggtgg tttgttggtg gcgctggcgc
     7561 tactagtact gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc
     7621 cgttaatgct ggctttcttc gccaccattg gcctgaacgc caacattgcc agtttgcgtg
     7681 ccggtgggcg tgtggttggc atcttcttga ttgtggttgt tggtctgttg gtgatgcaaa
     7741 atgccattgg cattggtatg gctagcctgt tagggcttga tccgctgatg gggctgttgg
     7801 ccggttctat tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt aaattgttca
     7861 ttgaacgtta tggcttcacc aatgcgacgg aagtggcgat ggcctgtgca acgttcggtc
     7921 tggtgctggg cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa cactccacca
     7981 cgccgaacgg tattccggat gaccaggaag tcccgacggc gtttgaaaag ccggatgtgg
     8041 gacgcatgat cacctcgctg gtgctgattg aaactatcgc gctgattgct atctgcctga
     8101 cggtggggaa aattgttgcg caacttttgg ctggcactgc ttttgaactg ccgaccttcg
     8161 tctgtgtact gtttgttggc gtgattctga gcaacggtct gtcaatgatg ggcttttacc
     8221 gcgtctttga gcgtgcggta tccgtgctgg gtaacgtaag cctgtcgttg ttcctggcga
     8281 tggcgctgat ggggctgaaa ctgtgggagc tggcttcgct ggcgctgccg atgctggcga
     8341 ttctggtggt acagaccatc ttcatggcgt tgtatgccat cttcgttacc tggcgcatga
     8401 tgggcaaaaa ctacgatgcg gcagtgctgg ctgcgggtca ctgtggtttt ggcctcggtg
     8461 caacgccaac ggcaatcgcc aacatgcagg cgatcactga acgctttggc ccgtcgcaca
     8521 tggcgttttt ggtggtgccg atggtcggtg cgttctttat cgatatcgtc aatgcgctgg
     8581 tgattaagct gtatttaatg ttgccgattt ttgccggtta aaaatgaaaa agcgtcggaa
     8641 ggaaccggaa acgctacgtg aacactgtcg ccatattttt ggtgatgagc cgccagtgct
     8701 ctgtgtatgg gaaacggaat ttgattacgc agatgccgaa ctcaaggcgc tggcggctaa
     8761 agagtggcag cagatcagcg aatgggattt gagtgcttac tatgtactca atctcgttta
     8821 taacgaaccg atgcaaatag agttgtttcg ctatctgttt ccgctctgcc tggcgcaatg
     8881 gcatgaaacc gttttagctg gcggttatgg tgatcacttc gaagaatcct taatgaaggc
     8941 attgtgtcgg ccctatctct ggcaggagat gatgaatgct tcccaacgcc agcaggtacg
     9001 gcaattttta ctggacaccg cgttgcagcg tatggataac gagcgtggtt tcaataacgt
     9061 tctttgctgg ctggcggtgt tcaatactct gggtggggcc gcgccgttaa ttcgttctct
     9121 ctggtcgcgc tggtgggcgc tggatacgcc aggaaaagcg gtgtgtgcta ttcagtatgc
     9181 cgcgcatcta atttatccca tagaagccaa tccgttatgg tcgcaagaat ggataggctg
     9241 ggggcatcca ttaggccata aagatggctg gtcctctgat aatcgcgctt ttcttcgcca
     9301 aatgttaaca cctgaaatga ttgtggctgg cgtacaggca gcggctgaaa ttctgcgtgg
     9361 tgaaccggaa ggcgcaatgg cggcgcgaat tgcacaggat gcgtatgagg caatggatat
     9421 cctcactatc cagattgaag atttactgcg tgacttgtct tgtgatgagt ctggtcatgc
     9481 gttggagtaa cgttccgtct ccggcatcca gcgttctatc agggcttttg cttgttgtgg
     9541 gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg cctgatcgcg
     9601 cagtaaatcc gccactttaa actcagcatt acccgtctga cgcgtaccta acagttcacc
     9661 cggtccgcga atctctaagt ctttctgtgc aatcacaaaa ccgtcgttgc tgtcgcgcag
     9721 cacttgcagc cgaatttgcg cggttttgga gagcggcgtt ttgtagagca gcacgcagtg
     9781 agaagccacc gcaccacgac ctacacgccc gcgaagctgg tgtaactgcg ccagacccag
     9841 acgctccggg ttttcgataa tcatcagact ggcgttaggc acatcaacgc cgacttcaat
     9901 aacggttgtg gcaaccagca ggtgtagctc accttgttta aacgacgcca tcaccgcctg
     9961 tttctcggca ggtttcatcc gcccgtgtac caggccaacg ttcaactctg gtagcgccag
    10021 tttcaactct tcccaggtag cttccgccgc ctgcgcttcc agcaattccg actcttcaat
    10081 caacgtacaa acccagtatg cctgacgacc ttcagttatg caggcgtggc gcacgcggtc
    10141 aatgatgtcg gtacggcggg tatcaggaat agcgaccgta gtcactggcg tgcggcctgg
    10201 cggcagctca tctatcaccg aggtatcgag atcggcatac gcagtcattg ccagcgtgcg
    10261 ggggatcggc gtggcggtca tgatcaactg atgcggatgg aagccctgct gctggccttt
    10321 ctcccacaat gccagacgct gatgtacgcc aaaacgatgc tgttcgtcga taatcaccag
    10381 cgccaggccg ttaaactgca cctgttcctg gaagatggcg tgtgtaccga caatcatctg
    10441 cacctgaccg ctggcgatgg cttcctgctg tgccagccgt gctttacctt tctgcttacc
    10501 ggcgagccag cccacttcga taccgagcgg tgcaaaccag ttgcggaagt tattggcgtg
    10561 ctgctcggcg agtaattcgg ttggtgccat caatgctacc tgtttgccgt gggcaatcgc
    10621 acgcaacgcg gcgagggcgg cgaccagcgt tttaccggaa cctacatcgc cctgcaccag
    10681 acgcatcatc ggcacatcca gcgccatatc gcgctcgatc tccgccacta cgcgtgcctg
    10741 tgcgcccgtt tgcttgaacg gtaaggcggc gaggagttta tttttcagcg tgtcattggc
    10801 gctcagcggc tgggcatgaa aacgctgtgc tccggcacgt aaggctaaca tgctgaggtt
    10861 gtgcgccagc agttcttcca gaatcagacg acgttgcgcc ggatgctgcc cggtttccag
    10921 atcgctaagc tgtagcgtcg gtggcgggcg gtgcaaagtg cgcaacgctt ccggtagcgt
    10981 catcattcct tgtgacagtt ccggcggcag gagttcttca atggcgcagg tgtcgagcag
    11041 atccagcgcc tggtcggtta atttacgcag cgtggcctgc tttacgcctt ccgttgttgg
    11101 ataaaccggc gtgagcgttt cctgtaattc tggcgtgctg agatcgccct gcacgcggta
    11161 ttccgggtgg atcatctccg caccatattt accgcgcttt gcttcgccat aagccagtac
    11221 acggcggccc gtcgccaggc tatttttcat tgccgcgctg aaattgaaaa agcgcatggt
    11281 gaggatgccg gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc cgaaggagat
    11341 attgcagttc agcacttcgc cttccaccgt ggcataaacg cccggcagta gttctccgat
    11401 ggggtagaga tgggtgcgat cttcgtagcg caggggaagg tgtaagagta gatcctgcac
    11461 ggtatgcagg ttgattttcg ccagtttgtt actaagtgct gcgccaacgc ccgttaggga
    11521 actgagtggg acagcatcta acaggcgacc tttcatggca cttaccctgc agcctgcata
    11581 gtagcccacc agtcggcatc agcttcgatc tcgccttgct gattgacgtg gggataaggc
    11641 aggcctttgc gttttgcgac tttcgccagc accggatagc cgccttcaaa caacaggcgt
    11701 tgttgctctg cttccggcaa catgctgttt tcacgcaggt acatgcctgc attttgccgc
    11761 tgacgctggg cttcgtaaag aatgagggct gaggcaacgg aaacattcag cgactgcacc
    11821 atgccgatca tcggaatgat gatgtcctga tccgccaggg ccaatgcttc ctgcgtgatg
    11881 cccgttttct cctgtcccat caaaatgcag gtcgggcgag tgtaatcaat ttcgcggaaa
    11941 tcgacagcgt tatcagaaag atgggttgcc agaatctgca tgccctggcc tttgagatga
    12001 gcgacggcat cgccaatggt gcggtgtgtt ttcacctgta cccagctgtt actacccgcc
    12061 gctgccgaag ccatggtgcg catgcggcta ccaggccaga cggcgtgaac ttcatgtacg
    12121 ccaacggcat ctgcggtacg aataatcgca gaaacgttat gaggtttgtg gacctgctcc
    12181 atgcagacgg tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg tgcataacgt
    12241 gttgggttca taaaacatta atttcggttt cgggtgactt taatcacgtc tggcatcacg
    12301 cggattttgc gcatgatatt cgccagatgc acacggtcac gggcggtcag acgaataaag
    12361 gcgctgtaga cgcgaccatc tttctcttcc gtattcaaac tttgaatatt cgaagtcgtg
    12421 gtgttaattg ccgccgtcag gtttgccagc gcgccctgat gattgaacat ctccaccttg
    12481 atttcggtga tgaactcctg cgccgtctct ttatcccatt ccacagccat aaacttctct
    12541 ggctctttct ggtagccacg gatattacgg caggattcat ggtggatcac cagaccttta
    12601 ccggggctga cgtgggcgat aatcgggtcg ccaggaatag ggcggcagca tttcgcaaag
    12661 gtgatcagca cgccatcggc acctttaatg ggcagatgtc cgtggctttg ggttgccggt
    12721 ggaatggagg cgtccccatg ttgcagattt ttcgcgacca ccacgctcat tgcgttacca
    12781 agtccgattt ctgccagcag atcgtcaagc gttgccagct tcatgcgatc cagctcgcgc
    12841 tgaatatttt cctgcgggat ttcattgagc ttacggctac cacccaaagc atggttgagc
    12901 agacgacggc ccaggcttac agaatcatca cgcttgaggt ttttcagcaa ctgacgaatt
    12961 ttggcgcgcg ctttcgagct aacgacaaag ttcagccaag cggcattcgg gcgagcgccc
    13021 ggagcggtaa tgatttcaac ggtctgaccg ctagtaagcg gctgcgacag cgggtaaggc
    13081 tggcggtcga cgcgtgcgcc cacgcaggca tggccgatat cggtatgcac tgcataagcg
    13141 aagtcgacgg gcgttgcacc ggcaggcagc tcgacaatgc gcccttccgg tgtgaaaacg
    13201 taaatctcat ccgggaagag atcggattta acgctctcga taaattcaaa cgaactaccg
    13261 gcgctctgtt gcagctccag caggctttgc atccagcgct gggcgcggat ttgtgcggta
    13321 gtactggttt cgccgtgctc tttataagcc cagtgcgcgg caacacccat ctccgccatc
    13381 tgatccatat cttcggtacg gatctggacc tcaaccggca cgccgtgcgg gccaatcatc
    13441 gaggtgtgca acgactgata gccgttcgct ttaggaatgg cgatatagtc tttcacgcga
    13501 cccggacgcg gcttgtacag gctgtgcatc tggcccagca cgcgataaca ggtgtcagaa
    13561 tcattgacga tcacgcggaa agcgtagatg tccatgattg agtgaaaacg ctgctctttg
    13621 agcaccattt tgcagtaaat cgaataaaga tgcttttcgc gaccactgac gcggcacggt
    13681 attcccgctt cctgcaaacg cccttcgatt tcagagagga ttttttggat catctcttta
    13741 cggttgccgc gcgcggcttt caccacttct ttaattacgc ggtaacgatt gggatacagc
    13801 gcctcaaaac ccagctcttc gagttcggtt ttaatgtggt ggatacctaa acggtgcgcc
    13861 agcgggctat aaatttcgag agtttcacgg gcgatgcggc gacgtttgtc cgggcgaagt
    13921 gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt tgatgaggat gacgcggata
    13981 tcctgcacca tcgccataat catcttgcga aagttttcgg cctgcgcctc tttcttatcg
    14041 cggaacttga gtttatcaag tttcgacacc ccctctacca gctcggcgac gcttttacca
    14101 aaaagctgtt ccatatcctg gtaggtggcg ggagtatctt caatcacgtc atgcagcagc
    14161 gccgccatca gcgtttcata gtcgagtttc atctcggcca gaatgcaggc aaccgctacc
    14221 gggtgcgtga tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc atcacgtgca
    14281 acgagatacg cctgccgcag acgcttgatt tggtcttccg gcaggtaggt ttgaatcagt
    14341 tgattcaggc tttcaaacag atacaagggc gacccgcttt gtgattaacg acgaccttca
    14401 gcaatagcgg taacggcttg taattcagcg gcttcctgct cttgctgttc ctggcgttcg
    14461 cgaacgtcga ggatctggtt gttgatcaga ccttcttcga tttcgcgcag cgcgattaca
    14521 gtggttttat cgttttcttc cggtaccagc ggatcctttc cgcctacctg catctgacga
    14581 gcgcgacgcg cggcgaccag taccaggtca aaacggttac caattttctc tacagcgtcc
    14641 tgaacagtta cgcgtgccat acttaaaaag ctccacaggt gaagaaatga ctgggcatga
    14701 tactgaaatc aggttcagtc tgccaacaat ttgctgatta aagcgtcatg acgctgcttt
    14761 tggcggctca tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt caacgcggta
    14821 tcgaagtcat cattcacaat cagataatca tattcggcgt aatggctcat ttctgcaaca
    14881 gcttgcgcca tacgctttgc aatgacctct tcgctgtcct gaccgcgacc gcgtagacgg
    14941 cggtccagtt caattttgga cggcggtaaa ataaagatac tccgcgcgtg cggcatcttc
    15001 tggcgaattt gctgcgcgcc ctgccagtcg atatcgagaa aaacatcgac accggtcgcc
    15061 agtacttgct caatggcctc acgcgaagtg ccatagtaat taccaaaaac ttctgcgtgt
    15121 tcgaggaacg catctctgct aatcatttct ttaaattcat catgattaac aaagaaataa
    15181 tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac agaaacctgg
    15241 gtgtcataca acggttgggt ttttaataaa gcctgaatca ggctggattt acccgcgcca
    15301 ctgggggcag aaacaatata aagcgtgcct tgagccatga gtatctttcg tatgtgatta
    15361 gcgaaataaa gcctacatac gagcttatta tacacggcgc ggccatgtga cgtagccctt
    15421 gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc tgtttccgca tttgcctgtg
    15481 atttactgca ataaagacga ttctcaggaa gcgtcctcgc agactccgtt tttgccactc
    15541 ggctttgcct tcaccacgtt gtatatctgc cagcctgact acagcggagg tgaagatgaa
    15601 agtatggatg gcgatattaa taagtatctt gtgctggcaa tcatctgtgt gggcggtctg
    15661 tccggcctgg tcgccagcca gagcacagga agaaatttca cgcctgcaac agcaaataaa
    15721 acagtgggac gatgactact ggaaggaagg aaaaagtgag gtggaagacg gtgtttacga
    15781 tcagttaagc gcccgtctta cgcagtggca acgctgcttt gtgagcgagc cccgcgacgt
    15841 gatgatgccg cctttaaatg gtgcggttat gcatccggtt gcccatacgg gtgtacgcaa
    15901 aatggcggat aaaaacgcat taagtctgtg gatgcgagaa cgtagcgatc tttgggtgca
    15961 gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg gacgggaaac tgaacaaagc
    16021 aatcagtcgc ggtaatggcc tgaaaggcga ggactggacg cagaaagtta gcttaatttc
    16081 cgctgtgccg caaaccgtta gcgggccttt agccaacagt acgcttcagg gggaaatatt
    16141 tctccagcgc gaggggcata tccaacaaca aatgggggga ataaatgccc gcgcaaaagt
    16201 tgctggcctg atgatgcgcc aggacgatag cgacacgctg aattctctgg gcgtttttgt
    16261 ctgggcatgg ccggatggac cgcagttaat gaccgatcgt ttaaaggagc tggctaccgc
    16321 aggttttact ctaacgcaga ggtatacccg tgcggttaaa aatgctgatg aggttgcgcg
    16381 cgtacgcaat gagtggtgga aagcgaaatt acccttcgtc accgatggcg tggttgtgcg
    16441 aggggcgaaa gaacccgaat cccgccattg gctaccaggc caggcagaat ggctggtggc
    16501 ctggaaatat caacctgtag ctcaggttgc cgaagtgaag gcgattcagt ttgcggtggg
    16561 taagagcggt aaaatatcgg tggttgcgtc actcgcacct gtcatgctgg atgataaaaa
    16621 agtccagcgg gtgaatattg gttccgtcag gcgctggcag gagtgggata ttgcgcctgg
    16681 tgatcagatc ctcgtcagcc ttgccggtca gggtattcct cgcattgatg atgtggtctg
    16741 gcgcggtgca gaacgtacaa aaccgacacc accagaaaac cgctttaact cgttgacctg
    16801 ctactttgct tctgatgttt gtcaggaaca gttcatttca cgcttagtct ggctggggtc
    16861 aaaacaggtt ctggggctgg atggtattgg tgaggccggc tggcgcgcgc tgcatcagac
    16921 tcatcgcttt gagcatatct tttcctggct tttattaacg ccagagcaat tacagaacac
    16981 gccggggatc gcgaaaagta aaagtgcgca gctatggcat cggtttaatc tggctcgtaa
    17041 gcagcctttt actcgctggg tgatggcaat gggaataccg ctaacccggg cggcgcttaa
    17101 tgccagtgat gaacggtcct ggtcgcaact tttatttagc acggagcagt tctggcagca
    17161 gctgccgggg actggatcgg ggcgcgccag acaggttact gaatggaagg aaaatgcgca
    17221 aatcaagaag ctgggcagtt ggctggctgc ccagcagatc acaggttttg aaccttagtg
    17281 tccttcgtgg ctgtagtaaa agaccggtaa tcccagctta aggcgtagcg ccagtaagcg
    17341 ggcaaaaaag ccgaatacca gggtggagat aattaccaca tcatggttag aaacatagtg
    17401 ttgcagcgcg atgtacagca cggcggaggc aaatgagaca ccggcataca actctttctg
    17461 gaataccagt gggatacgtt tacagaacat atcgcgcaaa acgccgccaa acacgccggt
    17521 ggttaccgcc gcgacaacgg caataattgg gccgtgcccc atatccagtg caacctgtgc
    17581 gccgatgata gaaaagacca ccagtccaag cgcatcgagc accagaaaca ctttgcgcag
    17641 gtaaggcatt acaggagcaa cgatagtggt gagtactgcg gcggtagcga cgataatcac
    17701 atattccggg tgtttgaccc agccgagcgg atagtggccc agcagaatat cgcgcactga
    17761 ccctccgcca attgcggtgg cggtcgcaat aataattacg ccaaatgtat ccatgcggcg
    17821 tcgcccggcc gccagagccc ccgtcatggc ttcggcagta atgccaacca aatacaaaat
    17881 gtgtaacagc atggttctct ccctaaaaat ctgtctggca gggtagcgat ttgtgcgtat
    17941 tgtcacgatt gagatttttt aaggtgagag gaaattgata acttgtagtt atgtgaaatc
    18001 gctattttct gtagcgtaga tgcattcttc tgacttcttg tctgagaaaa tgcataagaa
    18061 aaaataggtt aaaattgagg ggtggaaaag aaaggtgcaa tgtttagcca ttgtgttcac
    18121 cgcacctgga ctttggctta cgcatcgggt atctcaatca acaaaaagag tttttagttt
    18181 ctgctatttc ttctctgtaa tttttacact gttttccaac tgcttgatgc tgacctgcgg
    18241 ggtcggcaac tcctcaggca tggttccgcc aagttcctga atggtttgcc tcaccttgct
    18301 acccacgtca aagtgtgtgg tattagcttg ctgttttgaa ttcacgccat cccgcttgag
    18361 tttttcttct gtttgggtag ctcgaaagag attagccgcc agttctgttg aacccatatg
    18421 atccaggatc ttctgactct ttttcagccc cttcagttgg tggatggctt tctgatctaa
    18481 tccaccatac agcccctggt aaccatgatt ctgaaatatg gcgaaatcag tagctgtcgc
    18541 taccccagct tactgtgctg cctcaaccaa ttgtttgtta tgttctttca actcatttct
    18601 cagaaacaga cgcttttcgt cttcacgaag ttgcttaaat gcttcatcgt cagcaagctc
    18661 ctgccgtcgg gtctggatag caaaataagt ctgccctgcc gcaatgaccg gtttcgcagg
    18721 gtcgccgttt tgtactacca ggtaacaggc atagcgggag agatgaacat cttcaagttc
    18781 tcgttgagcc cctgaaccta atacaaccat tttggtggtt tcaacgaaat ggtcagatgc
    18841 agcttgattg ctggcttcgc acgcttgagt cgcgcgggcc agtacctttt gaaaattgcg
    18901 ccagtcccga taatccagta acggtgccaa ttctctagct gaccaaaatt cttgcccttc
    18961 agtgccataa tgccgtattt cttcaaaagg ttgatgatgt tcgttcattg cccactccat
    19021 gtgatatcga ttgcaaagaa agtaaatctg taactgtatt tatatacagt tgtgagtatc
    19081 tcacgttctc agtgtgctgg gcaaaaaaac atcgcaaaat ctggagcagg cacgcaaaac
    19141 agaattgaag ttgcggggaa gaaaaatgcc gctctgtttg agtgggcggc atttgtgaag
    19201 gctgaaaagt aagaattggc gttacgagtt attcaatgtt ctgaatctgc tcgcgcatct
    19261 gctcaatcaa cactttcagc tcaatcgcgg agtttgtcac ttcggcattg atagacttcg
    19321 acgcaagagt gttcgactcg cggttgaatt cctgcatcat aaagtccaga cgacgaccaa
    19381 cggcttcttt tttcttcaga atgttgtagg tctctttgac atgcgcttcc aggcggtcca
    19441 gttcttcggc aacgtcaatt cgttgtgcca gcagaaccag ctcctgctcc agacggttgt
    19501 tttccaactg aacctgagca tcttccagct tcgcgaccag acgctcacgc tgccattgca
    19561 ggatttccgg catatgggcg cggactttga ccacttcggc ggtgacgcct tccagacgct
    19621 gctcgatcaa tgctttcagt gcctgacctt cggtttcgcg cgcgacaata aagtcgtcca
    19681 gcgtaccatc cagcgccgcg agaatttcag cggcaatggc gtcaagatcc tgctcctggg
    19741 ctgccatcac gcccggccag cgtagaatat caaccgggtt gatttcccct tcgtcactct
    19801 gcatttttac ccagttcgcg gcagttacca gctgtttagc cagtttttcg ttgaggatca
    19861 gctcaccttg cgcgctaaca tctggctcat agcgcagggt acattccact ttaccgcgcg
    19921 tcaggcgaga acgaatacgc tcgcgaacga caggttcaag gctacggaac tgctccggca
    19981 gacgaaagta agtttccaga taacgctggt ttaccgagcg catttcccag gttgcgctcc
    20041 cccattcacc cttgatttca cgccgggcgt aggcggtcat actgcggatc atagacgttc
    20101 ctgtttataa aaggagaggt ggaaggatta tagccatcga tgccttgtca ggataggaat
    20161 aaccgccgga agtccgtata atgcgcagcc acatttgttt caagccggag atttcaatat
    20221 gcgtccagca ggccgtagca ataatcaggt gcgtcccgtt accctgactc gtaactatac
    20281 aaaacatgca gaaggctcgg tgctggtcga atttggcgat accaaagtgt tgtgtaccgc
    20341 ctctattgaa gaaggcgtgc cgcgcttcct gaaaggtcag ggccagggct ggatcaccgc
    20401 agagtacggc atgctgccac gttctaccca cacccgtaac gctcgtgaag cggcgaaagg
    20461 taagcagggt ggacgcacaa tggaaatcca gcgtctgatc gcccgtgctc ttcgcgcggc
    20521 agtagatttg aaagcgctgg gtgagttcac cattacgctg gactgcgacg tgcttcaggc
    20581 tgatggtggc acgcgtaccg cgtcgattac gggtgcctgc gtggcgctgg cagatgcgct
    20641 acagaagctg gtggaaaacg gcaagctgaa aaccaatccg atgaaaggga tggtagccgc
    20701 agtttctgtc ggaattgtga acggcgaagc ggtttgcgat ctggaatacg ttgaagactc
    20761 tgccgcagag accgacatga acgtagtgat gaccgaagac gggcgcatca ttgaagtgca
    20821 ggggacggca gaaggcgagc cgttcaccca tgaagagcta ctcaccttgt tggctctggc
    20881 ccgaggggga atcgaatcca ttgtagcgac gcagaaggcg gcgctggcaa actgattttt
    20941 aaggcgactg atgagtcgcc ttttttttgt ctgtagaaaa gtaagatgag gagcgaaggc
    21001 atgaaaccat atcagcgcca gtttattgaa tttgcgcttg gcaagcaggt gttaaagttt
    21061 ggcgagttta cgttgaagtc cgggcgcaaa agcccctatt tcttcaacgc cgggctgttt
    21121 aataccgggc gcgatctggc actgttaggc cgtttttacg ctgaagcgtt ggtggattcc
    21181 ggccttgagt tcgatctgct gtttggccct gcttacaaag ggatcccgat tgccaccact
    21241 accgccgtgg cgctggcgga gcatcatgac cttgacctgc cgtactgctt taaccgcaaa
    21301 gaggcaaaag accacggtga aggcggcaat ctggttggta gcgcgttaca aggacgcgta
    21361 atgctggtag atgatgtgat caccgccgga acggcgattc gcgaatcgat ggagattatt
    21421 caggctaatg gcgcgacgct tgctggcgtg ttgatttcgc ttgatcgtca ggaacggggg
    21481 cgcggcgaga tttcggcgat tcaggaagtt gagcgtgatt acaactgcaa agtgatctct
    21541 atcatcaccc tgaaagacct gattgcttac ctggaagaga agccggaaat ggcggaacat
    21601 ctggcggcgg ttaaggccta tcgcgaagag tttggcgttt aaagaaactc gccggatgaa
    21661 aagtcatccg gcgtcatatt actgcaactg tgccgcaatt agcggccagc gggcgtcaaa
    21721 atcatccgtc gggcggtatt taaattcgct gcggacaaaa cgtgacagca taccttcaca
    21781 gaaggccagg atctggcttg ccagcagggt ttcatcggtg gtgtaacctt caccctcacg
    21841 cattctcttt tcacgcaata cctgacgcag ctgcgcttca atacgctcga acagctggtt
    21901 gatgcgccct tgcaggcgat cctgttcaaa cattagcgca tgaccagtga ggatgcgggt
    21961 caggccagga ttacgctcac caaaaccgag aagcagcaac acaatcagac gcaggcgcgc
    22021 tgtggtgtct ttctcatctt tcagaatcag attgatgcga gtaatcaggc tatcttcgat
    22081 aaactcaatc aggctatcga acatgcgggt cttactgggg aagtggcgat acagtgccgc
    22141 ttcggaaacg ccgacagagg cggccagttt tgccgtcgtg atacgttggc ttccatcgct
    22201 ggattccagc atcagcgcca gagactgaag tatttcctcg cgacggttcc ttttcgcagt
    22261 ttgtttttct gccatgttac aaaatacccc tgaaaataag cacttgccag gcggcaccca
    22321 cgctatgacc gcaaacgaaa tgtttgcggc tatgttatga cgttattcgg atgcgtatgt
    22381 gttactgacg accagagtga ccaaagccgc cttcaccgcg gtcggtggcg tcgaaatctt
    22441 ccaccagatt aaattcagcc tgtactaccg gaacaaaaat catctgggcg atgcgttcgc
    22501 caggttgaat ggtgaagctg tcctgaccac ggttccacac ggaaatcatc aactggccct
    22561 gatagtcgga atcgatcaat cctaccaggt taccaagcac gataccgtgc ttatgtccca
    22621 atccggagcg cggcagcatc attgccgcca gtgaaggatc ggcaatatga atcgccagcc
    22681 cggtcggaac cagcgtagtg tcacccggag ccagttctac ggcgtcgtcg agacagacac
    22741 gcaggtcaag tccggcagag ccagaggtgg cataagtcgg gagcggaaat tccttcccaa
    22801 cgcgcgggtc cagaatctta acgtcgattt ttttcatcat aacgggtcac gatctcgtcg
    22861 agtaataatt ggccaaggag ctctttgcgc tcaagcggta agactttatc tccgtcctgc
    22921 cagaaaaggt gtaatgcgtt gttgtcgctg ttaaatcctt gagttggctg ggaaacatcg
    22981 ttcgcgcaga tcagatcaag gtttttacgg atacgttttt gccgggcgta ttcttccaca
    23041 ttatttgttt cggcggcaaa tccaacgacg tagggtcgat ggtcttttag tgcggcaacg
    23101 cctgcgacga tatcggggtt tttaaccatt tttattgtta attcatcacc ctgcgtggcc
    23161 tgctttttga ttttctctgg ggccacggta gctgcgcgat aatccgccac ggcggcgcaa
    23221 ccgataaaaa tattttgctg ctgtacagaa gcattcacgg cggcttccat ttccagcgca
    23281 gtcatcacat caacacgttt aacaaacggt ggcgtcggta gtgaaaccgg acctgatacc
    23341 agcgtgacgt tcgcgccacg acgggcagcg gcggcggcga tagcaaaacc catcttgccg
    23401 gagctgtgat tagagatata acgcaccgga tcgagcggtt cacgcgtcgg gccggcggta
    23461 atcataatgt tcagatgttt caggtcgttg acgggcgaaa aatgcgccac cgccatatcc
    23521 acaatggtta acggatcgag cattcgccca ggaccgatat caccacaagc ctgactgcca
    23581 ctgtctggcc cccagatgag cataccacgg gaagcaagca cctctaaatt atgctgcgtg
    23641 gcagcggcac ggtacatctg ctggttcatg gcggggagca cggctacagg cgcaggtgta
    23701 gccagacaaa tcgtcgatac caggtcattc gccattccgg cagcaatacg ggcaatcaaa
    23761 tctgccgtgg caggggcgag aatcactaaa tcagcccatt tacccagctc aatatggccc
    23821 atagcggctt cggctgccgg gtccagcaga ctgtcggaaa cgggataacc agaaaccgcc
    23881 tgcaagctaa gtggggtgat aaaggctttt gccgcttcgg tcatggctac gcggacgtcg
    23941 gccccgcgat cgcgcaaacg acgcaccagt tcaggggttt tataggcagc aataccgccg
    24001 ctaacgccga gaacgatttt tttaccggcc aggctcatga tgatttttcc tgtagggtga
    24061 caccagaagt tggcgatttt atcacaatac ttttgttgtc gtgccttcac ctgagattca
    24121 ctttgcgagg cgctttccag gattgaaaac tggccgtcga tttaacgaaa cggctatgac
    24181 aggatgcgag caccacaaag gaggtgaagg tgaaaaacaa ttcacagctg ttgatgccgc
    24241 gcgaaaaaat gctgaagttt ggtattagcg ccttaacgga tgtcgagctg ctggcgctat
    24301 ttctgcgtac cggaacgcgc ggtaaagatg tattaaccct ggcaaaagag atgctggaga
    24361 atttcggctc tctttatggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg
    24421 gaattggcgt ggcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact
    24481 acaacgtgcg gatgcgtgaa gaaagccctt tactcagccc ggagatgacg agggaatttt
    24541 tacaaagcca gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactccc
    24601 aacaccgggt tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc
    24661 atcctcggga aattatccgc gaagcgataa aaataaacgc ctcggcgctg atccttgcac
    24721 ataatcaccc ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga
    24781 taataaagag ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg
    24841 gagagtatgt ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg
    24901 gatctttgtc tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt
    24961 gagaatctcg ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga
    25021 ccgggctgta aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt
    25081 tttggagaat agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta
    25141 acaaccgttc ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc
    25201 accgtttctg ggttgagagc gagaagcgtt ttgtcaccct gcgcgtatct gctaaaggta
    25261 tgcgtgtaat cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg
    25321 aaaagtacta agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa
    25381 gctggtttct tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa
    25441 gccggaaaaa ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa
    25501 agaagcgaaa atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt
    25561 tttttgttat ctgcttgccc ccatattgac tgcatctgtt cattcctgga gatgctatgc
    25621 ctgaattacc cgaagttgaa accagccgcc gcggcataga accgcatctc gttggtgcaa
    25681 ccattcttca tgcagtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct
    25741 accgtttaag cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg
    25801 agctgcctga gggctggatt atcattcatt tagggatgtc tggcagcctg cgcatccttc
    25861 cagaagaact tccccctgaa aagcatgacc atgtggaatt ggtgatgagc aacggcaaag
    25921 tgctgcgcta caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag
    25981 ggcataatgt gctgacccat cttggaccgg agccgcttag cgacgatttc aatggtgagt
    26041 atctgcatca gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca
    26101 agctggtggt aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc
    26161 atccggatcg gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga
    26221 ttaaagcggt gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc
    26281 aaagtgatgg taaaccgggc tatttcgctc aggaattgca ggtttacggg cgaaaaggcg
    26341 agccgtgtcg ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt
    26401 tttattgtcg gcagtgccag aagtaattca tgcgcgccgg atggcatacc atccggcata
    26461 aacgctacgc taacttcgcc atcagcgcct gatggacatt ctccggcagg aaatgggtga
    26521 catcgccctg atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt
    26581 tcgacggcat cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca
    26641 gctgcatttc atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt
    26701 gttgattacg ggcgaagttc gccatcaaat cactaaaccc gaccacttcc acgttcccca
    26761 gatgcgcggt tgcctgctgt gccagcgcca cacgctcttc cagggtaaac atcggttttt
    26821 tactggggct ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca
    26881 cgatatcgat atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt
    26941 gcataacaac ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc
    27001 tgtagcgcgc cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg
    27061 taatcggcgt cggtgagtaa agaggacacc tcttttgcaa gcgtagtggc atcggtaacg
    27121 gtaatcagcc cgcttgcctg ctccagccgc gcgcaaatgt ctttaaagtt aaaagtatgc
    27181 ggccccatca ataccggaat agcgtgtgcg gcagcttcca gcggattatg cccaccacgt
    27241 tcaaccagtg aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg
    27301 cccatcgtat cgccaactac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt
    27361 gtgatatagc ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga
    27421 tgacggggta ccaggatgag caataaattc gggaattgct gtaacaatgc ctgatgtgcg
    27481 gcaatgacca cgctctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt
    27541 ggtgcccatt ggcggcgtag cgtcacggct ttagcagcca actgcggcgt tacagaaata
    27601 tcgaatttca ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa
    27661 cgtgcaccat cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag caagcggcgg
    27721 acgaatttac ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg
    27781 atcaccagcg gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt
    27841 tccataatca acaccagttt agggtcgact ttattcagga aacggttgag tgcatcgggc
    27901 aggtcatacg gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc
    27961 gaaccggttg gcgtcatggt tgtaacggta atcggtaaat caggataacg atgacgcagc
    28021 gcgcgcacca acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg
    28081 ccgcctggtt ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag
    28141 gccggagcct tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag
    28201 agaagggcgg tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg
    28261 attctatgta tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa
    28321 tttacccgcc aattcttgct gaacatctat actttttttg tatttttatg cgattcattg
    28381 atttctgctg tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa
    28441 tgattttatt tgattgattt ctttattgaa aatcaacaat gtgcttaaaa atcattcggg
    28501 tcggattgcc gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt
    28561 caggtagctg ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa
    28621 tctcaacaaa aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg
    28681 cgttttcatg gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac
    28741 cctgacgcaa aaatcgatgt gctgctttat caggacacca tcccgatcct gtctgaaaat
    28801 ccagagatta acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt
    28861 gccaactttt ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat
    28921 cttaccgatc aatggatggt tgctatactg gttcgcttat taaatgctcg tgtgaaaatt
    28981 tcccaggatt atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttggtg
    29041 ccgttgcagg gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt
    29101 gattcgttgg tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt
    29161 cgcgaacttg atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg
    29221 caaatcttca aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct
    29281 cgtggttatg aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat
    29341 gaaattgcgc agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc
    29401 ccggaacttg gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg
    29461 gcgcatattg ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagatcat
    29521 attttctggc gtccctggtc aaataacatg attcaattct gggcgggaga ttaccgggaa
    29581 atgccaacgc gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca
    29641 gatgtcattg ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga
    29701 tcgttgcttt ttgtttatat aaatattttc cctttggcgg tttgcagcgt gattttatgc
    29761 gtattgctca gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg
    29821 aaggcgaatg ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc
    29881 acgggcgcaa tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg
    29941 ataaagtcgt tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt
    30001 gttatgccga gaaagtagcg caggaaaaag gctttttcta tcgcctgacg tcacgttatc
    30061 gccattatgc cgcctttgag cgggcaacct tcgaacaggg taagccgaca cagttgctga
    30121 tgctgacaga taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt
    30181 ttcatattct gccaccgggg atttatcctg atcgtaaata tagccagcag ccagccaata
    30241 gccgtgaaat cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg
    30301 tcggttcaga cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac
    30361 cggattcgct gcgccacaac acattgctat atgttgttgg gcaggataaa ccgcgaaaat
    30421 ttgaggcgct ggcagaaaaa cgcggcgtgc gcagtaatgt tcactttttc tcggggcgca
    30481 atgatgtctc ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag
    30541 cggcgggaat tgtgctgctg gaagcgatta ctgcaggatt accggtacta acaacagccg
    30601 tttgtggcta tgcgcattat attgtcgacg ctaattgcgg cgaggctatt gctgagccat
    30661 tccgccagga aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc
    30721 aggcctgggc ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag
    30781 agaaagcggc ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc
    30841 acgttatggc gcggcaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggtattt
    30901 cgtgaactgg aaactcgccg tactctgcgc tttgaaatgg cgggcaaaag ctattttctc
    30961 aaatggcatc gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg
    31021 ccagtattag gcgctgaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt
    31081 gatactatgt atggggtggc atttggcgaa aaaggcatga atccgctgac cagaacttca
    31141 tttattatta ccgaagatct gacaccaacc ataagtcttg aagattacag tgctgactgg
    31201 gcgactaacc ctccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg
    31261 gtgcgcgata tgcatgctgc gggcattaac caccgtgact gttatatctg tcatttcctg
    31321 ctgcacttgc ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac
    31381 cgggcgcagc ttcgcacgcg cgttccacgt cgttggcggg ataaagatct tattgggctt
    31441 tatttttctt cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg
    31501 tattttgccg ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca
    31561 gaagcaaaag ccacaaaaat cagggaaaga acgattcgaa aatcgttgta acccgtaact
    31621 tatttttgcc aaaattttgg atacagaata aatatgtctc aactcaatga tagt
//
LOCUS       DAZFWP020000045        30882 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFWP020000045 DAZFWP020000000
VERSION     DAZFWP020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.045"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="ACHK0Z_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="ACHK0Z_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGU4501925.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1184..1567
                     /gene="secE"
                     /locus_tag="ACHK0Z_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             1184..1567
                     /gene="secE"
                     /locus_tag="ACHK0Z_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU4501926.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1569..2114
                     /gene="nusG"
                     /locus_tag="ACHK0Z_RS09490"
                     /old_locus_tag="pgaptmp_001898"
     CDS             1569..2114
                     /gene="nusG"
                     /locus_tag="ACHK0Z_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU4501927.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2273..2701
                     /gene="rplK"
                     /locus_tag="ACHK0Z_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             2273..2701
                     /gene="rplK"
                     /locus_tag="ACHK0Z_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU4501928.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2705..3409
                     /gene="rplA"
                     /locus_tag="ACHK0Z_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             2705..3409
                     /gene="rplA"
                     /locus_tag="ACHK0Z_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU4501929.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3822..4319
                     /gene="rplJ"
                     /locus_tag="ACHK0Z_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             3822..4319
                     /gene="rplJ"
                     /locus_tag="ACHK0Z_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU4501930.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4386..4751
                     /gene="rplL"
                     /locus_tag="ACHK0Z_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             4386..4751
                     /gene="rplL"
                     /locus_tag="ACHK0Z_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU4501931.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            5071..9099
                     /gene="rpoB"
                     /locus_tag="ACHK0Z_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             5071..9099
                     /gene="rpoB"
                     /locus_tag="ACHK0Z_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU4501932.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9176..13399
                     /gene="rpoC"
                     /locus_tag="ACHK0Z_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             9176..13399
                     /gene="rpoC"
                     /locus_tag="ACHK0Z_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU4501933.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13640..14773)
                     /gene="thiH"
                     /locus_tag="ACHK0Z_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             complement(13640..14773)
                     /gene="thiH"
                     /locus_tag="ACHK0Z_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU4501934.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGHASQRP"
     gene            complement(14770..15540)
                     /gene="thiG"
                     /locus_tag="ACHK0Z_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             complement(14770..15540)
                     /gene="thiG"
                     /locus_tag="ACHK0Z_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU4501935.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15542..15742)
                     /gene="thiS"
                     /locus_tag="ACHK0Z_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     CDS             complement(15542..15742)
                     /gene="thiS"
                     /locus_tag="ACHK0Z_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU4501936.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15726..16481)
                     /gene="thiF"
                     /locus_tag="ACHK0Z_RS09540"
                     /old_locus_tag="pgaptmp_001908"
     CDS             complement(15726..16481)
                     /gene="thiF"
                     /locus_tag="ACHK0Z_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU4501937.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16474..17109)
                     /gene="thiE"
                     /locus_tag="ACHK0Z_RS09545"
                     /old_locus_tag="pgaptmp_001909"
     CDS             complement(16474..17109)
                     /gene="thiE"
                     /locus_tag="ACHK0Z_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU4501938.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17109..19004)
                     /gene="thiC"
                     /locus_tag="ACHK0Z_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     CDS             complement(17109..19004)
                     /gene="thiC"
                     /locus_tag="ACHK0Z_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU4501939.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19085..19185)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19237..19713)
                     /gene="rsd"
                     /locus_tag="ACHK0Z_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             complement(19237..19713)
                     /gene="rsd"
                     /locus_tag="ACHK0Z_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU4501940.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19808..20581
                     /gene="nudC"
                     /locus_tag="ACHK0Z_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             19808..20581
                     /gene="nudC"
                     /locus_tag="ACHK0Z_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU4501941.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20621..21685
                     /gene="hemE"
                     /locus_tag="ACHK0Z_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             20621..21685
                     /gene="hemE"
                     /locus_tag="ACHK0Z_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU4501942.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21695..22366
                     /gene="nfi"
                     /locus_tag="ACHK0Z_RS09570"
                     /old_locus_tag="pgaptmp_001914"
     CDS             21695..22366
                     /gene="nfi"
                     /locus_tag="ACHK0Z_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU4501943.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYILGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22409..22999
                     /gene="yjaG"
                     /locus_tag="ACHK0Z_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             22409..22999
                     /gene="yjaG"
                     /locus_tag="ACHK0Z_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU4501944.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23186..23458
                     /gene="hupA"
                     /locus_tag="ACHK0Z_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             23186..23458
                     /gene="hupA"
                     /locus_tag="ACHK0Z_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU4501945.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23471..24166
                     /gene="yjaH"
                     /locus_tag="ACHK0Z_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             23471..24166
                     /gene="yjaH"
                     /locus_tag="ACHK0Z_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU4501946.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24168..24593)
                     /gene="zraP"
                     /locus_tag="ACHK0Z_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             complement(24168..24593)
                     /gene="zraP"
                     /locus_tag="ACHK0Z_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU4501947.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGGHMGMGHW"
     gene            24831..26207
                     /gene="zraS"
                     /locus_tag="ACHK0Z_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             24831..26207
                     /gene="zraS"
                     /locus_tag="ACHK0Z_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU4501948.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26204..27529
                     /gene="zraR"
                     /locus_tag="ACHK0Z_RS09600"
                     /old_locus_tag="pgaptmp_001920"
     CDS             26204..27529
                     /gene="zraR"
                     /locus_tag="ACHK0Z_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU4501949.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27526..28815)
                     /gene="purD"
                     /locus_tag="ACHK0Z_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             complement(27526..28815)
                     /gene="purD"
                     /locus_tag="ACHK0Z_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU4501950.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28827..30416)
                     /gene="purH"
                     /locus_tag="ACHK0Z_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             complement(28827..30416)
                     /gene="purH"
                     /locus_tag="ACHK0Z_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU4501951.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7236 a         8035 c         8515 g         7096 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaggaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggtcgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag ctaattgccg
      961 ataacatttg acgcaatgcg cactaaaagg gcatcatttg atgccctttt tgcacgcttt
     1021 cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg agacaggctc
     1081 tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg cctcgcgatc
     1141 gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg cgaataccga
     1201 agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg tggcattgct
     1261 cctggtggcg attgtcggca actaccttta tcgcgacatt atgctgccgc tgcgtgcgct
     1321 ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga caaaaggtaa
     1381 agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca tttggccgac
     1441 tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag taatgtcact
     1501 gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca ctggcctgag
     1561 gttctgagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg ttttccggtt
     1621 ttgaaggccg cgtagcaacg tcgctgcgtg agcatatcaa attacacaac atggaagatt
     1681 tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc ggtcagcgtc
     1741 gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg atgaacgacg
     1801 cgagctggca cctggtgcgc agcgtaccgc gtgtgatggg cttcatcggc ggtacttccg
     1861 atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc ctgcagcagg
     1921 ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc cgtgttaatg
     1981 atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag aaatctcgtc
     2041 tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac ttcagccagg
     2101 ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca caaggcgtga
     2161 gattggaata caatttcgcg ccttttgttt ttatgggcct tgcccgtaaa acgatttttt
     2221 atatcacggg gagcctctca gaggcgttat tacccaactt gaggaattta taatggctaa
     2281 gaaagtacaa gcctatgtca agctgcaggt tgcagctggt atggctaacc cgagtccgcc
     2341 agtaggtccg gctctgggtc agcagggcgt aaacatcatg gaattctgca aagcgttcaa
     2401 cgcaaaaact gattccatcg aaaaaggtct gccgattccg gtagtaatca ccgtttacgc
     2461 tgaccgttct ttcactttcg ttaccaagac cccgccggca gcagttctgc tgaaaaaagc
     2521 ggctggtatc aagtctggtt ccggtaagcc gaacaaagac aaagtgggta aaatttcccg
     2581 cgctcagctg caggaaatcg cgcagaccaa agctgccgac atgactggtg ccgacattga
     2641 agcgatgact cgctccatcg aaggtactgc acgttccatg ggcctggtag tggaggacta
     2701 agaaatggct aaactgacca agcgcatgcg tgttatccgc gagaaagttg atgcaaccaa
     2761 acagtacgac atcaacgaag ctatcgcact gctgaaagag ctggcgactg ctaaattcgt
     2821 agaaagcgtg gacgtagctg ttaacctcgg catcgacgct cgtaaatctg accagaacgt
     2881 acgtggtgca actgtactgc cgcacggtac tggccgttcc gttcgcgtag ccgtatttac
     2941 ccaaggtgca aacgctgaag ctgctaaagc tgcaggcgca gaactggtag gtatggaaga
     3001 tctggctgac cagatcaaga aaggcgaaat gaactttgac gttgttattg cttctccgga
     3061 tgcaatgcgc gttgttggcc agctgggcca ggttctgggt ccgcgcggcc tgatgccaaa
     3121 cccgaaagtg ggtactgtaa caccgaacgt tgctgaagcg gttaaaaacg ctaaagctgg
     3181 ccaggttcgt taccgtaacg acaaaaacgg catcatccac accaccatcg gtaaagtgga
     3241 ctttgacgct gacaaactga aagaaaacct ggaagctctg ctggttgcgc tgaaaaaagc
     3301 aaaaccgact caggcgaaag gcgtgtacat caagaaagtt agcatctcca ccaccatggg
     3361 tgcaggtgtt gcagttgacc aggctggcct gagcgcttct gtaaactaat gcctttacgt
     3421 gggcggtgat tttgtctaca atcttacccc cacgtataat gcttaatgca gacgtatatc
     3481 cgagatattc gggttgtggc aaggcggcaa ctgagtgagt cgccaggagc atagctaact
     3541 atgtgactgg tgcgaatgaa ggaagccaac gccgccacaa gttgaatagc gacggataga
     3601 aaagatttgt tcgttggagc ctggcctatc caggcctccg tcgaagaccg caggagtttc
     3661 gcaagaaact taatcccctg cgtagacggt gacagaacgc taagattatt cttttatatt
     3721 ctggcttgtt tctgctcacc gtaattaaga cgctctctcc gtttggagga gtgaagtgag
     3781 ttccagagat tttctctggc aaacatccag gagcaaagct aatggcttta aatcttcaag
     3841 acaaacaagc gattgttgct gaagtcagcg aagtagccaa aggcgcgctg tctgcagtag
     3901 ttgcggattc ccgtggcgta actgtagata aaatgactga actgcgtaaa gcaggtcgcg
     3961 aagctggcgt atacatgcgt gttgttcgta acaccctgct gcgccgtgct gttgaaggta
     4021 ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac cctgattgca tactctatgg
     4081 aacacccggg cgctgctgct cgtctgttca aagagttcgc gaaagcgaat gcaaaatttg
     4141 aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc gtctcagatc gaccgcctgg
     4201 caactctgcc gacctacgaa gaagcaattg cacgcctgat ggcaaccatg aaagaagctt
     4261 cggctggcaa actggttcgt actctggctg ctgtacgcga tgcgaaagaa gctgcttaat
     4321 cgcagttatc tttttaacgc attcgcttac gtataaactt attctgatat tcaggaacaa
     4381 tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt tgcagctatg tctgtaatgg
     4441 acgttgtaga actgatctct gcaatggaag aaaaattcgg tgtttccgct gctgctgctg
     4501 tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa aactgaattc gacgtaattc
     4561 tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc agtacgtggc gcaactggcc
     4621 tgggtctgaa agaagctaaa gacctggtag aatctgcacc ggctgctctg aaagaaggcg
     4681 tgagcaaaga cgacgcagaa gcactgaaaa aagctctgga agaagctggc gctgaagttg
     4741 aagttaaata agccaaccct tcccgttgca gcctgagaaa tcaggctgat ggctggtgac
     4801 tttttagtca ccagcctttt tgcgctgtaa ggcgccagta gcgtttcaca ctgtttgact
     4861 actgctgtgc ctttcaatgc ttgtttctat cgacgactta atatactgcg acaggacgtc
     4921 cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata gcaacaggca ttgcggaaag
     4981 tgttccattt tccggtcaac aaaatagtgt tgcacaaact gtccgctcaa tggacagatg
     5041 ggtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga gaaaaaacgt
     5101 attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct cctttctatc
     5161 cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta tggtctggaa
     5221 gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga gctgcaatac
     5281 gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat ccgtggcgtg
     5341 acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg cgaagcgccg
     5401 gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat tccgctcatg
     5461 acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc ccagctgcac
     5521 cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc gggtaaagtg
     5581 ctgtataacg cgcgtatcat cccttaccgt ggttcctggc tggacttcga attcgatccg
     5641 aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac catcattctg
     5701 cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa agttatcttt
     5761 gaaatccgtg ataacaagct gcagatggaa ctggtgccgg aacgcctgcg tggtgaaacc
     5821 gcatcttttg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg ccgtatcact
     5881 gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt cccggttgag
     5941 tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg cgagctgatc
     6001 tgcgcagcga acatggagct gagcctggat ctgctggcta agctgagcca gtctggtcac
     6061 aagcgtatcg aaacgctgtt caccaacgat ctggatcacg gcccatatat ctctgaaacc
     6121 ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta ccgcatgatg
     6181 cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa cctgttcttc
     6241 tccgaagacc gttatgactt gtctgcggtt ggtcgtatga agttcaaccg ttctctgctg
     6301 cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg acatcattga tgttatgaaa
     6361 aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca cctcggcaac
     6421 cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt tccgcgttgg cctggtacgt
     6481 gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct gatgcctcag
     6541 gatatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg ttccagccag
     6601 ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa acgtcgtatc
     6661 tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag gcttcgaagt tcgagacgta
     6721 cacccgactc actacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc gaacatcggt
     6781 ctgatcaact ctctgtccgt gtacgcacag actaacgaat acggcttcct tgagactccg
     6841 tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc actacctgtc tgctatcgaa
     6901 gaaggcaact acgttatcgc ccaggcgaac tccaacctgg atgaagaagg ccacttcgta
     6961 gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg cgaccaggtt
     7021 gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct gatcccgttc
     7081 ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg tcaggccgtt
     7141 ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc tgttgccgtt
     7201 gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt ggatgcttcc
     7261 cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg tatcgacatc
     7321 tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca gatgccgtgt
     7381 gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc gtccaccgac
     7441 ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg gaatggttac
     7501 aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg tttcaccacc
     7561 atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctggggcc ggaagagatc
     7621 accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga atccggtatc
     7681 gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt aacgccgaaa
     7741 ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg tgagaaagcc
     7801 tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac ggttatcgac
     7861 gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga aatcgaagaa
     7921 atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct cgaagcgggt
     7981 ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga gaagctcgac
     8041 aaactgccgc gcgatcgctg gctggagcta ggcctgacag acgaagagaa acaaaatcag
     8101 ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa gaaactcgaa
     8161 gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct gaagattgtt
     8221 aaggtatatc tggcggttaa acgccgtatc cagcctggtg acaagatggc aggtcgtcac
     8281 ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta cgatgaaaac
     8341 ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat gaacatcggt
     8401 cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa gatcaacgcc
     8461 atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg tgcgtacgat
     8521 ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga agaagttatg
     8581 cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt cgacggtgcg
     8641 aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc cggtcagatc
     8701 cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt tggttacatg
     8761 tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc caccggttct
     8821 tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg tcagcgtttc
     8881 ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct gcaggaaatg
     8941 ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa catcgtggac
     9001 ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt gaaagagatt
     9061 cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa acaggtcact
     9121 gctgtcgggg taaaacccgg cagcggattg tgctaactcc gacgggagca aatccgtgaa
     9181 agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg cgatcaaaat
     9241 tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta aaaagccgga
     9301 aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg cccgtatctt
     9361 tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga aacaccgtgg
     9421 cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc gtgagcgtat
     9481 gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat cgctgccgtc
     9541 ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac tgtactttga
     9601 atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga tcctgactga
     9661 agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga agatgggtgc
     9721 ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg aacagctgcg
     9781 tgaagagctg aacgaaacca actccgaaac caagcgtaaa aagctgacca agcgtatcaa
     9841 actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc tgaccgttct
     9901 gccggtactg ccgccagatc tgcgtccgct ggttccgctg gatggtggtc gtttcgcgac
     9961 ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc tgaaacgtct
    10021 gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc tgcaggaagc
    10081 ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt ctaacaagcg
    10141 tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc gtcagaacct
    10201 gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc catacctgcg
    10261 tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag ctgttcaaac cgttcatcta
    10321 cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga aaatggttga
    10381 gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac acccggtact
    10441 gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac cggtactgat
    10501 cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg ccgacttcga
    10561 tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg aagcgcgtgc
    10621 gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa tcatcgttcc
    10681 gtctcaggac gttgttctgg gtctgtacta tatgacccgt gactgtgtta acgccaaagg
    10741 cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct ctggtctggc
    10801 ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg caaacggtga
    10861 attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc tgtggatgat
    10921 tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa aagcaatctc
    10981 caaaatgctg aacacctgct accgcattct cggtctgaaa ccgaccgtta tttttgcgga
    11041 ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg ttggtatcga
    11101 tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag cagaagttgc
    11161 tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct acaacaaagt
    11221 tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg ataacctgca
    11281 aactgaaacc gtgattaacc gtgacggtca ggaagagaag caggtttcct tcaacagcat
    11341 ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc agcttgctgg
    11401 tatgcgtggt ctgatggcga agccggatgg ctccatcatc gaaacgccaa tcaccgcgaa
    11461 cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg ctcgtaaagg
    11521 tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc gtctggttga
    11581 cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag gtatcatgat
    11641 gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg tactgggtcg
    11701 tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc cgcgcaacac
    11761 gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg cggtaaaagt
    11821 acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct acggtcgtga
    11881 cctggcgcgt ggccacatca tcaacaaagg tgaagcaatc ggtgttatcg cggcacagtc
    11941 catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg gtgcggcatc
    12001 tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa ggtagcatca agctcagcaa
    12061 cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta acaccgaact
    12121 gaaactgatc gacgaattcg gtcgtaccaa agaaagctac aaagtgcctt acggtgcggt
    12181 actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa actgggaccc
    12241 gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg acatgatcga
    12301 cggccagacc attactcgtc agaccgacga actgaccggt ctgtcttcgc tggtggttct
    12361 ggattccgca gaacgtaccg caggtggtaa agatctgcgt ccggcactga aaatcgttga
    12421 tgctcagggt aacgacgttc tgatccctgg taccgatatg cctgcgcagt acttcctgcc
    12481 gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg acaccctggc
    12541 gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc cacgcgttgc
    12601 ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc cttgctgaaa tcagcggtat
    12661 cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc cggtagacgg
    12721 tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt tcgaaggtga
    12781 acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg acattctgcg
    12841 tctgcgtggt gttcatgctg tgactcgtta catcgttaac gaagtacagg acgtataccg
    12901 tctgcagggc gttaagatta acgataaaca catcgaagtt atcgttcgtc agatgctgcg
    12961 taaagctacc atcgttaacg cgggcagctc cgacttcctg gaaggcgaac aggttgaata
    13021 ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg gtgcaactta
    13081 ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct tcatctccgc
    13141 ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg gcaaacgcga
    13201 cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg caggtaccgg
    13261 ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc cggctgcacc
    13321 gcaggtgact gccgaagacg catctgccag cctggcagaa ctgctgaacg caggtctggg
    13381 cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
    13441 ttttttatgg gtgttattca gagggcaaat tcttcacgga atttttaaga aaggcagggc
    13501 gaaacgagga agaagctttt cgccctggac aacattcctg ctgacggaac taccataaaa
    13561 aaacagaacg ctttcaacaa ccttgcgttt ttcatcttcc gtagcggctc ggaataaatt
    13621 tacgttgtta cattctgtct catggccttt gcgaggcgtg tcccagatag ctgtcccagt
    13681 ctttccatac cggctgcaaa ccctgagcgg ttaacgcggc cgcaactgct tctggtctgc
    13741 gatcgtcgtg cggtgagaac tgttccagct cggggtgatt gtcggcatag ccacctggct
    13801 gcgttttcga aaaagcgctg acgttattaa ttgccagcgg aatcacgcga tcgcgaaacc
    13861 acggtgattc ccgcgtggag agtgagagtt caatctccgg tgcaagcagt cggaaggcgc
    13921 agatggtttg cactaactgg cgttcatcca taatcgacgc aggctcaatg ccgccagtac
    13981 acgggcgcag gcgcggaaag gagacagagt aacggctttg ccagtaatgc tgttgcagcc
    14041 atagcaaatg ttcggcaacc atatagcagt caacgcgcca gttgtcggaa aggccaatta
    14101 gcgcgccgag gcctatctta tcaatccccg cacgccccag ccgatccggc gtttccagcc
    14161 gccagaagaa gtcctgtttt ttgcctttca aatgatggcg ggcataagtc gcctcgtgat
    14221 atgtctcctg ataaaccatc acgccatcca gaccaagctg ctttaactcg gcgtattccg
    14281 tctccgccag cggttgcact tccatttgta gtgaagagaa ctgttcacgc agggcgggga
    14341 gatgacgacg aaagtaatcc atccccactt tcgcctgatg ttcaccagtg actaatagca
    14401 gatgttcaaa gcccatctcc cgtatagcgg cgctttccct ggcaatatcc gcttcatcca
    14461 gcgttttgcg tttgatgcga ttactcatgg aaaatccgca gtacgtgcag tcgttagcgc
    14521 aaagattgga aagataaagc gggacgtaga aactaactgt gttgccaaat cgctgacggg
    14581 tcagacgctg cgcccgttgg gccagttgtt ccagatagcc actggcggct ggcgataaca
    14641 gcgccatcat atcgtcgcgg gtgagttgcg aggcatttag cgcccgttct acgtcagcag
    14701 ccgttttgcc gttgatacgc aggcggatgt cgtcccagtc cagttgtcgc cagcgatcgc
    14761 tgaaggtttt catgccgatg cctccagaaa tccagtcagc gggctggtgg catgagcaaa
    14821 atgactgcgg ctgcccggtc cggactgacg tgccagcagg cctgcttcta ccgccagacg
    14881 aaatgccttc gccatgttga cgggatcgtc cgcgacggca attgccgtat tcactaacac
    14941 cgcgtcggcc cccatttcca gcgcctgcgc ggcatggctg ggaacgccga tgccagcatc
    15001 gacaaccacc ggcactgtgg cctgctggat aataatctcc agcatggcgc gggtttccag
    15061 tccctgattc gagccaatcg gcgcgccgag cggcatcacc gctgcacagc cgacttcttc
    15121 cagacgttta cacaataccg gatcggcccc gcagtaaggc agcacgacaa atccctgttg
    15181 taccagcgtt tcggcggctt tcagggtttc gatgggatcg ggcaacagcc agcgggcgtc
    15241 agggtgaatc tctaatttta accagtttgt gcctaacgct tcacgagcca gatgggcggc
    15301 gaaaatggct tcttccgctg ttttcgcccc ggatgtattt ggcagcaggg tcacacccgc
    15361 cgcgataagc ggttcgagga tagcgtcgtt gtgctggcgc aagtcgacac gtttcatcgc
    15421 cagtgtcacc agctggctgc cggaagcgcg gatcgcctcc accatcagtt gtgaagaagc
    15481 gaatttccct gtgccggtaa acagatgtga atcaaacgtt ttgtccgcaa tacgtaacat
    15541 ttcaaccccc tgcaataacc tgaaaaagca ggatctggtc gccatcctgc acgatatgtt
    15601 gcgcccactg ctcacgcggg acgatttgct gattaatcgc cagagccgcg cccgcttgtc
    15661 gttggtcgag ttgctccagt agttcgtgaa cagtttgccc ggcggcgcac tgcatcggtt
    15721 gatcgttaaa caggatctgc attgcttcct ccgcataccg ggcaaccact ggcgcggcgc
    15781 aacgccaggc tgcgccactg gctcgattta ccgtcgaaca gtcggagttc tcccgcaggt
    15841 gtctctatac cgcttaataa cttaatggct tccagtgcct gcaaagtgcc cataaccccg
    15901 accaccgggc caaccacgcc cgccgtgcgg cagttgcgtt ctggctcctg gttatctggc
    15961 cacaggcagc ggtaacaccc ctgctcccag ggcggcgtca gtaccatcaa ctgaccgcca
    16021 aatccgaccg cgctggcggt gataagcggc gtgttgagtg ccacgcaggc ggcattaatc
    16081 tcctggcgag tcgccatatt gtcggtacag tcgagcacca catcggcccg tgcaaccgca
    16141 tcttttaacg cctcacccgt taaccgttgt tgtaatgctg tcagttgaat gtcgggattc
    16201 aactgtgtca gtcgctgttg gctgacctgc gatttcgggc tgttgatatc ttcagtggta
    16261 aagaggattt gtcgttgcag attgcttaaa tgcacatcgt cgtcatctgc cagtaccagc
    16321 gtcccgacgc cagcgcccgc caggtacagc gcagcaggtg tacccagccc gcccagaccg
    16381 ataatcagca cctggctgtc gagcagtttt tgctgcccgt ccagagcgat atcgtcgagc
    16441 aggatttggc ggctataacg cataaagtca cggtcattca tcgccaactc ctgcaatttc
    16501 cagcaactgt gccgttgcca aacgccagtc tgcagcttga gtaatggcgc tgacgacggc
    16561 gatactgccg acacccgttg ctatcaccgc aggcgcgcgt gccagactga taccgccaat
    16621 cgccacggtg ggataatccg ccagtcgctc aacatgccgt gccagctgtt ccagcccctg
    16681 cggtgcagaa ggcatctgtt tggtttgcgt cgggaacaca tgtcccagcg cgatataaga
    16741 ggggcgtgct gccagcgcga cgtcgatttc catatcgtca tgtgtcgaaa cgcccagccg
    16801 caggcctgcc gcgcggatgg cattgagatc ggtggcttgc aaatcttcct gccccaaatg
    16861 gacgccatac gcctgatgct tgatcgccag ccgccagtaa tcgttgataa acaatcgcgc
    16921 gttatagcgg cggcccagcg caattgccgc cacgacatcg gcttccacct cttcatcgcg
    16981 ccgatctttg atgcgtagct ggagagtacg tacgcctgca tccaacagac gttcgatcca
    17041 ctgtacgctg tccaccaccg ggtacagtcc taaacgaaaa ggtacaggag gaaaatcagg
    17101 ctgatacatc acgcttcctc cttacgcagg tagatttctc cgcctctggc acggaagttc
    17161 tccgacatat ccgccattcc catttcaata gtttgcgtgg cggcgtaatc acgcacttcc
    17221 tggctgattt tcatcgaaca gaatttcggc ccacacatgg agcaaaaatg ggcgacttta
    17281 cctgactctt gcggcagggt ttcatcgtga taagcgcggg cggtaaacgg gtcgagggcc
    17341 agattaaact ggtcttccca gcgaaattcg aagcgggctt tcgacatggc gttatcgcga
    17401 atttgcgcgc ccggatgccc tttcgccagg tcagcggcgt gggcggcaat cttataggtg
    17461 ataagcccct gcttaacatc ttctttatta ggcagaccca gatgctcttt tggcgttacg
    17521 taacagagca tcgcgcagcc aaaccagcca atcatcgccg caccaatccc cgacgtgaag
    17581 tggtcatagc ccggcgcaat atcggtagtt agcggcccca gagtgtaaaa cggcgcttcg
    17641 tggcagtgct ctaactcctc ggtcatattg cggcggatca tctgcatcgg cacgtgacct
    17701 gggccttcaa tcatcacctg cacgtcatat tcccaggcaa ttttggtcag ttcgcccagc
    17761 gtatgcagct cggcaaactg cgcttcatcg ttggcgtcct gaatagaacc ggggcgcaga
    17821 ccgtcgccca gcgacagcga aacgtcataa gcggcacaga tttcacaaat ttcgcggaag
    17881 tgttgataga ggaaattttc ctgatgatgg gagaggcacc atttcgccat aatcgaaccg
    17941 ccgcgagaga cgataccggt caggcgtttc gcggtcatcg gcacatagcg cagcagtacg
    18001 cccgcatgga tagtgaagta atccacacct tgctcggcct gttccagcag cgtgtcgcgg
    18061 aacgcttccc aggtaagatc ttcggcgatc ccgttaacct tctccagcgc ctggtagatc
    18121 ggcactgtac cgatcggcac cgggctgtta cgcaaaatcc actcgcgggt ttcgtgaata
    18181 tagcgaccgg tggagagatc catcaccgta tccgctcccc agcgcgtgga ccataccagc
    18241 ttttccactt cttcttcgat ggaagaggtg accgccgagt tgccgatatt ggcgttaact
    18301 tttaccagga aattgcgacc aataatcatc ggctccgatt ccggatgatt aatgttggcc
    18361 gggataatcg cacgtccggc agcaacttca tcacggacaa attccgcagt gatattttcc
    18421 ggcagatgtg cgccaaagct cattcccgga tgctggtggc gtaaaacctc gctacggatg
    18481 cgctcgcggc ccatattctc gcggatggcg atgaattcca tttccggcgt gatgatgccc
    18541 tggcgggcgt agtgcagttg ggtgacacgg cgtcctgctt tggcgcgttt tggtgttagt
    18601 acaccgctaa aacgcagttc gtcgaggcca tcatctgcca gccgcgcttt agtgtaatcg
    18661 gaactgcgca cggtaagttc ttcggtatcg ccgcgcgcat cgatccacgg ctggcgtagt
    18721 tttgccagcc cttgctgcac gttaatggca atctgcggat caccatacgg gccggaggtg
    18781 tcgtagaccg gaatcgcttc gttttcttcg tactgcggct gttctttgct accgccaatt
    18841 agcgtcgggc taagctggat ctcacgcatc ggcacgcgca cgccggggtg tgtgccagtg
    18901 atataaatgc gttttgagtt gggaaaggcg gtgccttcca gggtgtcgat aaaatgttgg
    18961 gcccgggcgc gttgttcgcg gcgggtcagt tttgttgcag acatagctca ttccaaaaag
    19021 ttaaggacgt ggcttgtcag acgacggatg aagcaagaga cgatcgccgc aggggcgatg
    19081 cgatagcaga ttaactcttg ttcccttcgc aggtattagc ctgatcaggt tccgcggatc
    19141 ccgaataaac ggtctcagcc agttaaggca ctccgacaag aaattagccc cgcaaatggg
    19201 gcattgaatg taaattacgc gttaacagcg cagaactcaa gcaggatgtt tgacgcgggc
    19261 ggcgtcaagc accagcagaa tcagcttatc ttccagcaca aagcgcgctt ccagcgcctc
    19321 gccgatgtca gataaaacct gttgaaactc aaggtaatta tcatgatcga tggcggtttc
    19381 cagactggaa tcgtagtaat ccataatctg ttgagtattg gcttcgagtt gcggccaaat
    19441 cttcgcggcg cgcgcgagtt gcccgttgcc ttccagctta tgaagaatgc gctcataaat
    19501 actgaaatgt ccggcagaca agtaatcgac caggctctga caaaaatcat caagggcttt
    19561 ttcgtttagc ctcatgtacg attctttgcc aggcttaatg ccaaccagat tgtagtaagc
    19621 cacgagcaga tgcttacgta catgtagcca gcgatcaacc agtttgttac ttcctctgac
    19681 gcgctccgtc aggttatcga gctggttaag catgattgac tccgcaagtt tgtattcaaa
    19741 aactgctcag tgagaaatgt aaaaaccatg ttaaacatgc cagtgatgca aaggtagtgc
    19801 aagagctatg gatcgtataa ttgaaaaatt agatcacggc tggtgggtcg tcagccatga
    19861 acaaaaatta tggttgccga agggagaatt gccatatggc gaagcggcaa atttcgatct
    19921 tgtgggtcag cgcgcactac aaatcggcga atggcagggg gaacctgttt ggttagtaca
    19981 acagcagcgg cgtcacgata tggggtcggt acgtcaggtc attgatctcg atgttgggct
    20041 gtttcaactg gccggacgag gcgtacaact ggcggagttt taccgatcgc ataaatactg
    20101 tggttactgc gggcatgaaa tgtatccgag caaaaccgaa tgggcgatgc tgtgcagcca
    20161 ttgccgtgag cgttactacc cgcaaatcgc cccctgcatt attgttgcca tccgtcgcga
    20221 tgattcgatc ctcctcgccc agcatacccg ccatcgtaac ggtgtccata cagtacttgc
    20281 cggattcgtc gaagtgggcg aaaccctcga gcaggcagtc gcgcgggaag tgatggaaga
    20341 gagcggaatt aaagttaaaa acttgcgtta cgtgacttct cagccgtggc cgtttcctca
    20401 gtctttaatg accgcgttta tggcggaata tgacagcggc gacatcgtga tcgacccgaa
    20461 agaattgctc gaggcgaact ggtatcgcta tgacgatttg ccgttactcc cgccgcccgg
    20521 caccgtagcg cgccgtctga tagaagatac ggtggcgatg tgtcgggcag agtatgagtg
    20581 atgatacact gaccgcctga cgcactaagg aacagccaaa atgaccgaac ttaaaaacga
    20641 tcgttatctg cgggcgctgc tgcgccagcc cgttgatgtc actccagtat ggatgatgcg
    20701 ccaggcgggt cgctatctac cggaatataa agccacgcgc gcccaggcgg gcgattttat
    20761 gtcgctgtgc aaaaacgccg agctggcgtg cgaagtgact ttgcaaccgc tgcgtcgcta
    20821 cccgctggat gcggcgatcc tcttttccga tatcctcacc gtgccggacg cgatggggtt
    20881 agggctctat tttgaagccg gagaaggtcc gcgttttacc tcgccagtca cctgcaaagc
    20941 cgacgtcgat aaactgccaa ttccggaccc ggaagatgag ctgggttacg tgatgaacgc
    21001 ggtgcgtacc attcgtcgcg aactgaaagg cgaagtgccg ctgattggtt tttccggcag
    21061 cccgtggacg ctggcaacct acatggtgga aggcggcagc agcaaagcct tcaccgtgat
    21121 caaaaaaatg atgtatgccg atccgcaggc gctgcacgct ctactcgata aactggcgaa
    21181 aagcgtcact ttgtatctga atgcgcagat taaagccggt gctcaggctg tgatgatttt
    21241 cgacacctgg ggcggcgtgc ttaccgggcg cgattatcaa cagttctcgc tttattacat
    21301 gcataaaatt gttgatggtt tactgcgtga aaacgacggt cgccgcgtac cggtcacgct
    21361 gtttaccaaa ggcggcggac agtggctgga agccatggca gaaaccggtt gcgatgcgct
    21421 gggcctcgac tggacaacgg acatcgccga tgcgcgccgc cgtgtgggca ataaagtcgc
    21481 gttgcagggt aatatggatc cgtcgatgct gtacgcgccg cctgcccgca ttgaagaaga
    21541 agtagcgact atacttgcag gtttcggtca cggcgaaggt catgtcttta accttggtca
    21601 cggcattcat caggatgtgc cgccagaaca tgctggcgtg ttcgtggagg cagtgcatcg
    21661 actgtctgaa cagtatcacc gctaaggagt gattatggat ctcgcgtcat tacgcgctca
    21721 acaaatcgaa ctggcttctt ctgtgatccg cgaggatcga ctcgataaag atccaccgga
    21781 tctgatcgcc ggagccgatg tcgggtttga gcagggcgga gaagtgacgc gagcggcgat
    21841 ggtgctgctg aaatatccct cgcttgagct ggtggagtat aaagttgccc gcatcgccac
    21901 caccatgcct tacattctag gttttctttc cttccgcgaa tatcctgcgc tgctggcagc
    21961 gtgggagatg ctgtcgcaaa agccggattt agtgtttgtc gatggtcatg ggatctcgca
    22021 tcctcgccgt cttggcgtcg ccagccattt tggcttattg gtggatgtgc cgaccattgg
    22081 cgtggcgaaa aaacggctct gcggtaaatt cgaaccgctc tccagcgaac cgggcgcgct
    22141 ggccccactg atggataaag gcgagcagct ggcctgggtc tggcgcagca aagcgcgctg
    22201 taacccgttg tttatcgcta ccggccatcg ggtcagcgtg gacagcgcgc tggcgtgggt
    22261 acaacgctgc atgaaaggct atcgtctgcc ggagccaacg cgctgggcgg acgcggtggc
    22321 ctcggaacgt ccggcgttcg tgcgctatac agcaaatcag ccctaattca ggtaaactgc
    22381 gggtaatttc cgtatttgag aactcaacat gttacaaaac ccaattcatt tgcgtctgga
    22441 gcgcctggaa agctggcagc acgtcacttt catggcttgc ttatgcgaac gcatgtaccc
    22501 caattacgcc atgttttgcc agcaaaccgg ttttggtgat gggcaaattt accgtcgtat
    22561 tctcgatctc atctgggaaa cgctgaccgt taaagacgca aaagtaaatt tcgacagcca
    22621 actggagaaa tttgaagaag cgattccttc agccgacgat ttcgatctgt acggcgttta
    22681 tccggcaatc gatgcctgcg tggcgttaag tgaactggtc cattcgcgtt tgagtggtga
    22741 aacgctcgaa cacgcggtgg aagtgagtaa gacctccatc acgaccgttg cgatgctgga
    22801 aatgactcag gctggtcgtg aaatgagcga tgaagagctt aaagaaaacc cagctgtaga
    22861 gcaagaatgg gacattcagt gggaaatatt ccgactttta gctgagtgcg aagaacgcga
    22921 tatcgagctg ataaaaggcc ttagggcaga cctgcgtgag gcgggtgaga gcaatattgg
    22981 tataattttt cagcaataag accagaaaac gtgatttaac gcctgatttg tcgtacctgg
    23041 agtcttccct ttcgcccccc gtctggtcta catttggggg gcgaaaaaaa gtggctatcg
    23101 gtgcgtgtat gcaggagagt gctattctgg catttccgtc gcactcgatg cttagcaagc
    23161 gataaacaca ttgtaaggat aacttatgaa caagactcaa ctgattgatg taattgcaga
    23221 gaaagcagaa ctgtccaaaa cccaggctaa agctgctctg gagtccactc tggctgcaat
    23281 tactgagtct ctgaaagaag gcgatgctgt acaactggtt ggtttcggta ccttcaaagt
    23341 gaaccaccgc gctgagcgta ctggccgcaa cccgcagacc ggtaaagaaa tcaaaattgc
    23401 cgcagctaac gtaccggcat ttgtttctgg caaggcactg aaagacgcag ttaagtaaga
    23461 ttgcgtggca gtgaacagtt ttaacgaagg ggtggtttca ccccttttgt ctttctggcg
    23521 tcgatcattg atgctggcag gcgctctgtt tctcactgcc tgtagtcaca actcttcact
    23581 tcctcccttt accgccagtg gatttgctga agaccagggc gcggtacgca tctggcgaaa
    23641 agacagcggc gataatgtgc atctgcttgc cgtgtttagc ccgtggcgca gtggcgatac
    23701 cacgacgcga gagtatcgct ggcagggcga taacctcacg ctcatcaata tcaatgttta
    23761 cagcaaaccg ccggtgaaca ttcgcgcgcg ttttgacgat cgcggcgatc tgagctttat
    23821 gcaacgtgaa tccgatgggg aaaagcagca gctttctaac gaccaaatcg atttataccg
    23881 ttatcgtgct gatcagatcc gccagattag cgatgcctta cgtcagggga gagtcgtgct
    23941 gcgccagggg cgctggcatg cgatggaaca gaccgtgacc acctgcgaag ggcaaaccat
    24001 taaacctgat ttagattcgc aggcgatagc gcatatcgag cgccgccaga gccgctcttc
    24061 tgttgatgtc agcgtggcat ggctggaagc gcccgaaggt tcgcaattac tgttagtggc
    24121 aaactctgat ttctgtcgct ggcaacccaa cgagaaaacg ttctgattta ccagtggccc
    24181 atacccatat gaccgccacc accgcagccg ccgtaaccca tgcccattcc ggtaccgcgc
    24241 ggaatacccg cttcagccat cgcaatatct cgtttcaccc gtaactcatc taacgactga
    24301 cgcaaattct ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg
    24361 gctaacaggg cattgtattc ataacgcttc gtcaccagtt gctgttgcag tgcgctgctt
    24421 tgagcgtaaa agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc
    24481 gcggcatttt gctgccacat accgtgtccg ccgtgagcaa atgcagatgt cgatcccatc
    24541 gccattgctg aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat
    24601 cctccagtgg ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat
    24661 atgacgggta atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag
    24721 tcatttttac tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa
    24781 agatggcatg atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta
    24841 tgcaacgttc taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg
    24901 ttgggctggt agggttgttt gcggtgacgg tgattcgcga ttatgggcgc gagactgccg
    24961 ccgccagaca aacgctgctg gaaaaaggca gtgtacttat tcgcgctctt gaatccggct
    25021 cgcgcgtcgg catggggatg cgcatgcatc atgcgcagca gcaggcctta ctggaagaaa
    25081 tggccgggca gcctggagta cgttggtttg cggtcacgga tgaacaagga acaatcgtga
    25141 tgcatagcaa ctccggcatg gtgggaaaac agctttattc cccgcaggaa atgcagcagt
    25201 tacatcctgg agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc
    25261 tggaaattta tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata
    25321 tgcagcagta tgccgcaaca ccacaagcaa ttttcatcgc tttcgacgcc agtaatattg
    25381 tgagtgccga agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg
    25441 tcttgctggc aagcgtattg tcattcttct ggtatcgccg ctatctgcgc tcgcgccagc
    25501 ttctacaaga tgaaatgaag cgcaaagaga agctggtggc gctggggcat cttgcggcag
    25561 gcgttgccca cgaaatccgt aacccacttt cctcgattaa aggactggcg aaatactttg
    25621 ccgaacgcgc gccagcaggg ggagaagcgc atcaattggc gcaggtgatg gcgaaagaag
    25681 ccgaccgttt aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg
    25741 ctttgcaggc ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg
    25801 atgcaaacag ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc
    25861 aggccgatcc ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg
    25921 cgattggtca gcatggcgtg attagcgtga cggtcagcga aagcggcgcg ggcgtgaaaa
    25981 tcagcgttac cgacagcggt aagggaattg cggcagatca gcttgaagcc atcttcactc
    26041 cgtacttcac caccaaagcc gaaggcaccg gattggggct ggcggtcgtg cataatattg
    26101 ttgaacaaca cggtggtaca attcaggtcg caagccagga gggaaaaggc tcaacgttca
    26161 ccctctggct tccggtcaat attacgcgta aggacccaca aggatgacgc acgataatat
    26221 cgatattctg gtggtggatg atgacattag ccactgcact attttgcagg ctttactgcg
    26281 cggctggggc tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg
    26341 ggaacaggtt tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcatcgc
    26401 cacgctgaaa gagatcaaag cgttaaaccc ggcaattccg gtgctgatta tgactgcgta
    26461 ctccagcgtc gagacggcgg tagaggcact gaaaactggg gcgctggatt atctcatcaa
    26521 gccgctggat ttcgataacc tacaggcgac gctggaaaag gcgctcgcgc atacgcacag
    26581 tattgatgct gaaacacctg cggtgactgc cagccagttc ggtatggtcg gtaaaagctc
    26641 ggcgatgcaa cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacggtact
    26701 gatccacggc gattccggca ccggtaaaga gctggtcgcc agggcgattc acgccagtag
    26761 cgcacgtagc gaaaaaccgc tggtaacgct caactgtgcg gcactcaacg aatccttgct
    26821 ggaatctgaa ttgttcggtc acgaaaaagg ggcgtttact ggagccgata aacgccggga
    26881 agggcgcttt gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc
    26941 gccgatgatg caggtgcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg
    27001 tagcaaccag actatctcgg ttgatgtccg gctgattgcg gcgacccatc gcgatcttgc
    27061 cgcagaggtg aatgccgggc gttttcgcca ggatctctac tatcgcctga atgtggtggc
    27121 gattgaagta ccatcgctgc ggcaacggcg ggaagatatc cctctgctgg ctggccattt
    27181 tctgcagcgc tttgccgagc gtaatcgcaa ggcggtaaaa ggttttacgc cccaggcgat
    27241 ggatctgttg attcattacg actggccggg aaatattcgt gagctggaaa acgcggtgga
    27301 acgggcagtg gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat
    27361 tgccagtacg ccgatcccgc tggggcaaag tcaggatatt cagccgctgg tggaagtgga
    27421 aaaagaggtg attctggcgg cactggagaa aacgggcggc aacaaaactg aagccgcccg
    27481 tcagttaggg atcacgcgta aaacgttatt ggcaaaactg tcgcgttagt tctgctcgcg
    27541 ttcgatagca cgccagccga tatctttccg gcagaagcag tcgtcccagt ggatatcggt
    27601 cattaaggca taggcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtgacgca
    27661 cagtacgcgt ccgccgttgg ttaccacctg ctcgtcatcc gccagttttg tgcccgcgtg
    27721 gaacactttg ccgtcagcca cttcttccag cggcaggccg tggatcacat cgccggtgcg
    27781 gtaatcgccc ggatatccac ccgcagccat caccacgccg agagaagcgc gttcatccca
    27841 ctcggacgtt ttctcgtcca gcttgccttc acaggccgcc aggcagagct caaccagatc
    27901 ggacttcatg cgcagcataa tcggctgggt ttccggatcg ccaaagcggc agttaaattc
    27961 aataaccttc ggattgccct gtttgtcgat catcaggccc gcatagagaa aaccggtgta
    28021 ggtgttgcct tccgccgcca tgcctttcac ggttggccag ataatacgtt ccatggtgcg
    28081 ctgatgaacg tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgccggtgtt
    28141 tggtccggta tctttatcgc ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac
    28201 atgctcgccg tccaccatca cgataaagct cgcttcttcg ccatcgagga actcttcgat
    28261 aacgatgcga tgacccgcgt cgccaaaagc gttgcccgcc agcatatcgt gaacagccgc
    28321 ttccgcttct tccagcgtca tcgccacgat aacgcctttc ccggcagcca gaccgtccgc
    28381 tttaatgacg attggcgcgc ctttctcacg cagatacgcc agcgcaggtt ctacctcggt
    28441 gaagttctgg tattccgccg tagggatctt atggcgggcc aggaaatctt tggtaaacgc
    28501 ttttgagcct tccagttggg ccgcacctgc ggttgggccg aagattttca gcccggcggc
    28561 gcggaaggta tcgaccacgc ctttcaccag cggcgcttcc gggccgacga tggtcagatc
    28621 aatcttttcg ttttgtgcga aatccaacag cgccgggata tcggtcacgc caatagcaac
    28681 gttttgcagc gcgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtctc
    28741 aaccagcggc gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat
    28801 cactaatact ttcatctatt gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc
    28861 atcgcaatac cgtgctcgtc ggcggcggca atcacttcgt catcacggat ggaaccgcca
    28921 ggctggatta cgcaggtcac gcccgcagcg gcggcggcat caataccgtc gcggaacggg
    28981 aagaacgcgt cagaagccat cgaggaacct ttcacttcca ggccttcatc agcagcttta
    29041 ataccggcga ttttcgcgga gtacacgcgg ctcatctggc ccgcgccaat accgatggtc
    29101 atattgtttt tggcatagac gatagcattg gatttcacga acttcgccac tttccagcag
    29161 aacagcgcat cgcgcagttc ctgttcggtc ggctgacgtt gggtgacgac gcgcagttct
    29221 tccgcaccga ccatccccag gtcacgatcc tgaaccagca gaccgccgtt cacgcgtttg
    29281 aaatcaagac ccggaacacg ctcgccccac tgaccgcagg tcagaacgcg tacgttctgc
    29341 ttggcggcgg tgattttcag ggcttcttcg ctggcggaag gcgcaataat cacttcgaca
    29401 aactgacgag aaatgatggc ctgtgcggtt tccgcatcca gctcgcggtt aaaggcaatg
    29461 atgccgccga atgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg
    29521 gaattgccga tagctacacc gcaagggttg gcgtgcttca caatcacaca tgccggctcg
    29581 gcgaactctt tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg
    29641 gctttaccct gaacctgggt tgcggtagca acggaggctt ctttcacatt ctcttctata
    29701 tagaaggcag cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag
    29761 ttcaggttca gcgtgcgtgg gaagcgacca gcggcttctt tgctttcacc gtggtacgcc
    29821 ggaaccatgc tgccgaagta gttggcaatc atgctgtcgt aggcggcagt gtgttcgaag
    29881 gctttgatgg cgaggtcgaa acgggttgca agcgtcagcg atccttcgtt gtcatccatc
    29941 tctttaataa tggcgtcgta gtcgctgctc ttcaccacga ttgcgacatc tttatggttc
    30001 ttagcggcgg agcgcaccat ggtcgggccg ccgatatcga tgttctcaac cgcatcttcc
    30061 agcgagcaac cttcacgggc cacggtctgg gcgaacggat acaggttaac aaccaccata
    30121 tcgataggct ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga
    30181 atgccaccat gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa
    30241 ccggtgtaat cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggcagtg
    30301 ccccctgtag acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata
    30361 ccggctttgt cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta
    30421 aatcccctgg atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc
    30481 taacgcccct aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca
    30541 acgctcgcga atttttctct ttcaatggtg atcacaattt tgacagtggt taccgtggga
    30601 aaaatacaga aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg
    30661 gggttttgct ggggaatgca gcagtcagtc atttttctgc aatttttcta ttgcggcctg
    30721 cggagaactc cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa
    30781 cagccggttc ggttgaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag
    30841 cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cg
//
LOCUS       DAZFWP020000046        30347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFWP020000046 DAZFWP020000000
VERSION     DAZFWP020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.046"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..371)
                     /locus_tag="ACHK0Z_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             complement(<1..371)
                     /locus_tag="ACHK0Z_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501952.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVQTALENLGLGEAAKRDVG"
     gene            complement(371..1051)
                     /locus_tag="ACHK0Z_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             complement(371..1051)
                     /locus_tag="ACHK0Z_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HGU4501953.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     DISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGVLL"
     gene            complement(1048..2247)
                     /locus_tag="ACHK0Z_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             complement(1048..2247)
                     /locus_tag="ACHK0Z_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4501954.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNTAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNSVIWRLPASVVVGVDGTVMATATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGSPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVTGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVMDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQAISDYINSLMIG
                     DSVLLSRIYSPANLGVVSGGNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            complement(2247..2600)
                     /locus_tag="ACHK0Z_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             complement(2247..2600)
                     /locus_tag="ACHK0Z_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501955.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVKSEA"
     gene            complement(2600..3352)
                     /locus_tag="ACHK0Z_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             complement(2600..3352)
                     /locus_tag="ACHK0Z_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp138 family membrane-puncturing spike protein"
                     /protein_id="HGU4501956.1"
                     /translation="MAISDQTRSGDLAETFKSERETTKNQIRVALPGIVQSFDPDAVT
                     AVVQPAIRSVETDNDGNRVTKNYPLLVDVPVVFPRGGGCTLTFPVKAGDECLVIFADR
                     CIDFWWQSGGIQETVDDRMHDLSDAFCIVGPQSQARRISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDRVN"
     gene            complement(3415..3585)
                     /locus_tag="ACHK0Z_RS09640"
                     /old_locus_tag="pgaptmp_001928"
     CDS             complement(3415..3585)
                     /locus_tag="ACHK0Z_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501957.1"
                     /translation="MTTSNNDNSQQSKPTQAEQSMADYESSIMLEVPFGGLDYMTESV
                     KEPELDNFKQKK"
     gene            complement(3589..4143)
                     /locus_tag="ACHK0Z_RS09645"
                     /old_locus_tag="pgaptmp_001929"
     CDS             complement(3589..4143)
                     /locus_tag="ACHK0Z_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501958.1"
                     /translation="MSKRSSIDTTEEQKAIETIGADFISSYYKDRYEEAKSTYRRLED
                     KVNYLLAILAVETSALLAVFGTVDFKSGLKSNWFLTLSILCAYLCFVCIVMCFFFLWR
                     TWGLKDIPKMPIHKRKEEHDFLFYGDKIKTLRYHMFQYGKAVELIESLHKEKAVYARY
                     LFEGIALSFGFFILSVTFLLINKA"
     gene            complement(4151..4498)
                     /locus_tag="ACHK0Z_RS09650"
                     /old_locus_tag="pgaptmp_001930"
     CDS             complement(4151..4498)
                     /locus_tag="ACHK0Z_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501959.1"
                     /translation="MKRWIFSLLALVSVGVSANTITMQCGNFRMDAIPDSLFKINGET
                     VTSQKVKMLGKDGTGMQIKMDLMPAKDGNNYGFEYIHRPGTKTRFLNVQLLQNSMDAP
                     KIIGSFPCKKVVG"
     gene            complement(4501..5565)
                     /locus_tag="ACHK0Z_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             complement(4501..5565)
                     /locus_tag="ACHK0Z_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501960.1"
                     /translation="MSQNWMRHFELQLVDGNGQGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGREFSKVRLIAGYDGIAPEVSASDVGIVREVDAADVGQSDGR
                     NYGLIFSGEIRYSVTGKDSPVDSYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVFPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     NNEYVHEAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     NNDIAMAGGQITDQNTDGNITLSGTTSQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAQDMPSSTAFQRGL"
     gene            complement(5568..5870)
                     /locus_tag="ACHK0Z_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             complement(5568..5870)
                     /locus_tag="ACHK0Z_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501961.1"
                     /translation="MAIQEIPLTADNQQFSIVLGGVTWRISIIWRDLYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLVVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            complement(5870..6457)
                     /locus_tag="ACHK0Z_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             complement(5870..6457)
                     /locus_tag="ACHK0Z_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HGU4501962.1"
                     /translation="MDILSTLFHQQSRKIGMIVPCVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLDLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVLISRTQQISVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            complement(6457..8445)
                     /locus_tag="ACHK0Z_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             complement(6457..8445)
                     /locus_tag="ACHK0Z_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HGU4501963.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYWASQRTGATVQGIQSIGYAVSQVGGSVDAARSSLESL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSSMPYYRANQYAQMLG
                     IDENTLMAMRRGVGGFSGQYSAMAKAIGFNADEAARSSNKFMTSLREFGAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILALGDIIGRLFFRLIEGTSG
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKTTVNDLVKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVSIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQHAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKVTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
     gene            complement(8435..8611)
                     /locus_tag="ACHK0Z_RS09675"
                     /old_locus_tag="pgaptmp_001935"
     CDS             complement(8435..8611)
                     /locus_tag="ACHK0Z_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP pyrophosphohydrolase"
                     /protein_id="HGU4501964.1"
                     /translation="MPDGLSYLLNPVDAGLIPYTALKDGSVDLYDIALLNDHLAVKAD
                     NQRRIEKWREDNER"
     gene            complement(8623..9075)
                     /locus_tag="ACHK0Z_RS09680"
                     /old_locus_tag="pgaptmp_001936"
     CDS             complement(8623..9075)
                     /locus_tag="ACHK0Z_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4501965.1"
                     /translation="MEFEIKGVKYRTAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKFEQLKPVFETMLPRIAEELSSLTEDDTDAIIHPCLAVVSRRHMDGWV
                     PVFTQGELMFDDIDLLVMLQLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            complement(9079..9519)
                     /locus_tag="ACHK0Z_RS09685"
                     /old_locus_tag="pgaptmp_001937"
     CDS             complement(9079..9519)
                     /locus_tag="ACHK0Z_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HGU4501966.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGADGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            complement(9530..10675)
                     /locus_tag="ACHK0Z_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             complement(9530..10675)
                     /locus_tag="ACHK0Z_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HGU4501967.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIQDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGIG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSNVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            complement(10679..11242)
                     /locus_tag="ACHK0Z_RS09695"
                     /old_locus_tag="pgaptmp_001939"
     CDS             complement(10679..11242)
                     /locus_tag="ACHK0Z_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501968.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWIRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVDAPVIFFGD"
     gene            complement(11217..11606)
                     /locus_tag="ACHK0Z_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             complement(11217..11606)
                     /locus_tag="ACHK0Z_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HGU4501969.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(11593..12147)
                     /locus_tag="ACHK0Z_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             complement(11593..12147)
                     /locus_tag="ACHK0Z_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501970.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAESA
                     LNRAGTLAVNGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(12144..12551)
                     /locus_tag="ACHK0Z_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             complement(12144..12551)
                     /locus_tag="ACHK0Z_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HGU4501971.1"
                     /translation="MSKNQSLPAVSDFRRDFPQFADPAKYPDAQIGFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            complement(12517..12885)
                     /locus_tag="ACHK0Z_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             complement(12517..12885)
                     /locus_tag="ACHK0Z_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501972.1"
                     /translation="MAKEKLVTIHVHTPFTLTLGDQSKQEFGRGRHNVPEEVASHWFT
                     QAHSELSESVISDTDDLQPIIDGLQAQIADKDKLIADLKDALLKLQEQNDSLQAQITA
                     ARTGGNGAKDVKESKSASGK"
     gene            complement(12926..13867)
                     /locus_tag="ACHK0Z_RS09720"
                     /old_locus_tag="pgaptmp_001944"
     CDS             complement(12926..13867)
                     /locus_tag="ACHK0Z_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HGU4501973.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDTGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETLSYVDGI"
     gene            complement(13879..14385)
                     /locus_tag="ACHK0Z_RS09725"
                     /old_locus_tag="pgaptmp_001945"
     CDS             complement(13879..14385)
                     /locus_tag="ACHK0Z_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501974.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANG
                     NAEISWKI"
     gene            complement(14389..15609)
                     /locus_tag="ACHK0Z_RS09730"
                     /old_locus_tag="pgaptmp_001946"
     CDS             complement(14389..15609)
                     /locus_tag="ACHK0Z_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HGU4501975.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCRDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(15624..16358)
                     /locus_tag="ACHK0Z_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             complement(15624..16358)
                     /locus_tag="ACHK0Z_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HGU4501976.1"
                     /translation="MTNRRNRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIE
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNRATEAVVTGGRAEYFAKEIAASGDIAKSRADLI
                     ARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDGM
                     TGHAGELPNCRCYKEIVFPTSQSYPA"
     gene            complement(16249..17715)
                     /locus_tag="ACHK0Z_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             complement(16249..17715)
                     /locus_tag="ACHK0Z_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HGU4501977.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMIDPVLTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ESDDEPQEPSGGNPLSNQPTQDSAGGRRHRKWSLRWFK"
     gene            complement(17715..19337)
                     /locus_tag="ACHK0Z_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             complement(17715..19337)
                     /locus_tag="ACHK0Z_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HGU4501978.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPVLKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWSEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMINYAPMNSALNIWELLGRQA"
     gene            complement(19340..19912)
                     /locus_tag="ACHK0Z_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             complement(19340..19912)
                     /locus_tag="ACHK0Z_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4501979.1"
                     /translation="MTKKLKAKHEVFCREFLVDLNATQAAIRAGYVSRRAHVTGAELY
                     GKPEIRARINELKQERIDQLGIDANYVLMRLVEIDRLDVADILEDDLSIKPLSAWPES
                     WRRYLSGFNLAEMFEGRGDDREMVGILKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(19974..20498)
                     /locus_tag="ACHK0Z_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             complement(19974..20498)
                     /locus_tag="ACHK0Z_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501980.1"
                     /translation="MKLTYKIVIAAFFFSAFGALVWSANHYHSKYQAEKLRADKAEGE
                     AEYQGKVIANQALNFNRFNQIAENASRLNSLVDIGHEKTVIKYREVLLREKNCDFPVP
                     VDIAVGLLNYANRLRASALHADSGDIDSAGDRATTTRTLTYCQAVLWINPLLAAIEKA
                     NNQLAGVRQIEQSR"
     gene            complement(20482..20958)
                     /locus_tag="ACHK0Z_RS09760"
                     /old_locus_tag="pgaptmp_001952"
     CDS             complement(20482..20958)
                     /locus_tag="ACHK0Z_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4501981.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKDAGGVVNEADL"
     gene            complement(20962..21303)
                     /locus_tag="ACHK0Z_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             complement(20962..21303)
                     /locus_tag="ACHK0Z_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HGU4501982.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIVMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGGSKE"
     gene            complement(21749..22090)
                     /locus_tag="ACHK0Z_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             complement(21749..22090)
                     /locus_tag="ACHK0Z_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501983.1"
                     /translation="MALISVRNRFESFMEQRYPDLSLQVKGNIGASMKAQLGIRIERE
                     LYSEDVTYCDSAVQLMWTLFQAGVLAERRATSVTLPALKAKPDSFYDAGYNEGIQDCR
                     KHLTASGIKVR"
     gene            complement(22122..22544)
                     /locus_tag="ACHK0Z_RS09775"
                     /old_locus_tag="pgaptmp_001955"
     CDS             complement(22122..22544)
                     /locus_tag="ACHK0Z_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4501984.1"
                     /translation="MRNIQQVLERWGGWAASEGGSVYFPPVAAGFKNLLPATQSGRLK
                     CSDNDGLIINSAMSCLKKKDPYLCTLLEWHYVQAMPVRAMGEKLGVSHTHVLKRLQAA
                     EGFIDGCLAMLDVVLEMDQSVQSKPQAIRTLRRSCSAA"
     gene            complement(22831..25017)
                     /locus_tag="ACHK0Z_RS09780"
                     /old_locus_tag="pgaptmp_001956"
     CDS             complement(22831..25017)
                     /locus_tag="ACHK0Z_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HGU4501985.1"
                     /translation="MSDSKPWGATPDEWFHFDLVLGRTAHLLPVVCNPSATISPDSKL
                     KALGKTPSRYNRDRQVTGIAQWTGHVVTEHDFARWSNEPDYGICVRTGHGWLALDCDS
                     EDEDIQADIRKTLVQLLGESPPRRWRANSNKCLYLLAVDGDFRKRIHRLAGDMGIIEL
                     LANGQQFVACGTHSSGARIEWDGGLPDEPPAITGEQLETLWQRLAEQLPVSVTTEAGN
                     TKMRDRSAFTPGATDDTAEYLDANGWTLLDGANGERYIRCPFEDGHSSGGDPTSTVYF
                     PAGTAGFEQGHFKCLHASCAHRDDGDFLNAIGIRNDDFEDLTSIEVAEPLPLPAFERD
                     KWGRIEATISNAAKAVVRSDFVDIDIRFDQFRDEIMFAPAGSGQWRAFTDADYARLRI
                     TMEKRGFKPVGRELIRDVVLLAADEQPFDSAITWLNGLEWDGVPRIECFYHTHFGTAD
                     TPYTRAVSMYMWTALAGRVLEPGIKADMVPILVGPQGCGKSSGVEALSPDPAFFTEIS
                     FAEKDDDLARKMRGRLVAEIGELRGLNTKELESIKAFVTRTHENWIPKYREFATQFPR
                     RLVFVGTTNEDEFLADKTGNRRWLPVEVSKVDVKAIKRDLLLLWAEAREVFQRLGGIQ
                     FREAEQLAASVHEQYTIKDAWLETVEKWLDTPDLMTNELPRNCEFLRASDVLRDAIGL
                     NPDRIGKREEMRISNVLQNCGYKRAQRRIGGKKCKVWEPLEPRGTT"
     gene            complement(25021..25221)
                     /locus_tag="ACHK0Z_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             complement(25021..25221)
                     /locus_tag="ACHK0Z_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4501986.1"
                     /translation="MSGLTPQEMVKSLIDSGYTQNQIAEIAGVKQSSISRLLTGVHSD
                     PRFSTVRAIEKLFQEVTARQKV"
     gene            25363..26037
                     /locus_tag="ACHK0Z_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             25363..26037
                     /locus_tag="ACHK0Z_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4501987.1"
                     /translation="MIDTTDILSQNIKYLMDKAKISSITELARRLQLNQPTLHRLVSG
                     EVKDPKYATLKQIADYFHVSPIDLAERSLQEMEKEDATGIKTYISLRFNKVPVLGNTQ
                     LGVGGLWSDTQYSVGSSDGFIYWPTKDEDAYALKCVGDSMMPRIKEGEFVIVEPNHDY
                     TPGDEVLVVTREGEVMVKTFLFERDGLFHLMSVNEDHPPVRVPRENIEKIHYVAGIAK
                     SALRMY"
     gene            complement(26456..26980)
                     /locus_tag="ACHK0Z_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             complement(26456..26980)
                     /locus_tag="ACHK0Z_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501988.1"
                     /translation="MSEEKGLVRRITEAATSAGGALKGAVDLAKEVNALQVDYNVKSK
                     TIDLLDKLIDARTGQFALTELLNEAKQRIVELESLLEKKQDWDSEKMNYEMYHPITNT
                     VVYVLKPTDDPEFKPHYLCTTCYESGMKSILQYHTSNAAYKILKCHKCSAEYKFPRNI
                     EVSGKTPAPLQPAR"
     gene            27205..27354
                     /locus_tag="ACHK0Z_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             27205..27354
                     /locus_tag="ACHK0Z_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501989.1"
                     /translation="MKPEYLHRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            27351..28253
                     /locus_tag="ACHK0Z_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             27351..28253
                     /locus_tag="ACHK0Z_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4501990.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATETENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRVFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNHDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            28256..29557
                     /locus_tag="ACHK0Z_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             28256..29557
                     /locus_tag="ACHK0Z_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HGU4501991.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSYPEHYAGCNVSMFLGSYPLRENPNDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGSELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERALEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIISNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTIVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            29573..30121
                     /locus_tag="ACHK0Z_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             29573..30121
                     /locus_tag="ACHK0Z_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HGU4501992.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWEPIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
BASE COUNT         7466 a         8279 c         7075 g         7527 t
ORIGIN      
        1 cccacatccc ttttcgccgc ttctcccaaa ccaaggtttt cgagagccgt ttgcacagtg
       61 ccatccgatt tgatatcgcc aaacggattc ttgcggctca ggtattcaac agcaaacccc
      121 gatcccagca attcaacaaa accgggcaga tcaccattat caagcacatc ccgttgcgtt
      181 ttgtcactta caaactgggc cagagctgca gcaataaagc tggcctgtcg aatggctttg
      241 ttcacctgcg cactggatgc tttccctgct gtaaatccgg ataaaagcgc aggcaacgct
      301 tcccattcct cctgcgacat aacattggca tttcgatcag ttgcaaacgc tttaaagtca
      361 tttttcgcca tcagagtaat actccccatg ctcctacatc aaaaccactg atgaattcgt
      421 tatccatatc aaaaccaaaa aattttgagc cttccgatgg ggtttccacc gaaggagttt
      481 caatgccccc cgcccatacc ccggcggctt ttactgtgag atacccctgt ttaattgccg
      541 caattaactc acgcgataca tctgaaatat cagtatcagg aaagacccag accgatatcg
      601 tcatgtcctg gttatcgact atctgcattc gcagcccgga tcctgctgtt gccgcgtcaa
      661 gaattgccgg aagcgaatca ttccgtccgt cccagttatt aatcgcaatc ttcgctttaa
      721 ggatgacacg ataagtttca tcgctgaggt acatgtatcc ggaatcagga tcgtatggtc
      781 cctgccatac accctgatca tatccaagcc cgtcggtatc ccagctgaaa taaacacctg
      841 agataggctg gctgacaaca cggctacgtc cgatccacaa tcccagaatg tcaagttgca
      901 caccaaccgc agagtcaata tcaaatgcag taatcagccc tctggtggcc gccgcaacat
      961 caataagcgg ccgggtcatc agatcaacat gtgcaagaaa tttaggtttg gtggcgtggt
     1021 agttcgtgat tagttcggtg tatttgctca tgactccacc gttataacga tattttccgg
     1081 ggtacaggac gcagattcgt tgtatctgat atcaatgttt gatgacgaca aagcccccgg
     1141 agatttccca atcgtcagtt cctgaatatc gtaatagcgt gcattcccgc cactcaccac
     1201 gccaagattc gccggtgagt aaatgcgact taaaaggacc gaatcaccaa tcatcagact
     1261 attgatatag tcggaaatag cctgctggat ctgctgccct atctgtgagg tataacccgt
     1321 aaaaactttt aatttaatcc gggcataaac aggcacatca ctggaacgcg agaatttgat
     1381 tacatgggga ttgccgtatt tatccggaac cgtaacggat gttgtaccgt gagtggctgt
     1441 cccctggcct ttattccctc tgatagcctg agcaatatcc atcacatcac cgccatccac
     1501 aattacagca acagagtgtg gcggtaaccc gttaccgtcc tccgaaccag tatcgttttc
     1561 atagagtttg tggcgggtta caccggtaac attagaaaca gcaccatcca gtgcttcaaa
     1621 tggggttatt gatggcaacg caacactttg cgactggcgg atacgtaact ccgcatcagt
     1681 ttctgccgga gagccaacag tagccgcagc aggattagtt accgaaaccc agccacgggt
     1741 tggcgtatta atttcagtga tagttccagc cagcgccgcc actgcaccac tgacggaaca
     1801 tgttgcggtc gccatcactg taccatccac gccgaccacc actgaagcag gcaaacgcca
     1861 tatcacacta ttactgtctt tcacgctgcc attaatgatg gttgttccgg cagttcctgt
     1921 aagaagcaaa tcaaccgtag aattcgtcgc gcctttacgt gaaataccat ttattttcac
     1981 gttactggtc agtgcggccc catagccggt tgccggtgaa aaacagttgt agacagttat
     2041 cgcagtatta ttggcatcat gaatcgccag cgccatcaga gccaccatct ggccgtcttt
     2101 actatccggt tcgaggtagg catcactgcc ataaatctgc tgaaaatagc taatcagggt
     2161 gctgagtatc gtctgataat caggcgcact gatcccctcc gcggttacct ttgcagataa
     2221 accgagagaa tcaaggttca gagccattac gcctccgatt taacagtcgt tattccatag
     2281 agagtgtcga tttcagcgga aaacatgaca cgtcgggtcg tggtatccac cgtcgtattg
     2341 aaagagagga ttgatttaac gccccgcgtt tcgaggatgc gcttacggat cgccaggttg
     2401 taggtttccg gcttctgctt accgagcaca gactggatcc acggggtccc ttcggtggtg
     2461 tcgagaaacc attgcccata ccacaattca aatcgcgttt ttaccgcctg cgccacggcc
     2521 tccggtgagt taatcagcca ggtgtcatca ccgctgccaa aggtgtaatc gccatcggcg
     2581 tcttcacgtc tgtatcgcat cagtttaccc tatcggtatt gcttccaccg cgctgaacac
     2641 cgccatgagt gtgcgtatca tcgattggct tgccgttagc cttcacgctc cccaaaaact
     2701 caacagcacc agtgattttt gaagccacac cagaaaccac agaccccacc atgcccccca
     2761 tccaggttaa caggccatga atggttactt tctcagaaaa atcagccaga ggggcaacca
     2821 catcaagacc acccggagcg acaattttaa ttttcctggt atcaggatta agctcaaaat
     2881 aggtgctgcc gtcatcacta cgcaactgtg tggcactggt attaataccg ctaatcctcc
     2941 ttgcctgcga ctggggaccg acaatacaaa acgcatccga taaatcatgc attctgtcat
     3001 cgaccgtctc ctgtatcccg ccactctgcc accagaaatc gatgcagcga tcggcaaaaa
     3061 tcaccaggca ttcatcgccg gcttttaccg ggaaagttaa cgtgcagccg ccgccgcgcg
     3121 gaaataccac cggcacatcc accagcagcg ggtaattttt ggttacgcgg ttgccgtcgt
     3181 tgtcggtttc aaccgaacga atagcaggct gcacaaccgc cgtcaccgcg tcaggatcga
     3241 atgactgaac gatgccaggc aaagcgacgc ggatctggtt ctttgtggtt tcccgctcgg
     3301 atttgaatgt ttcggcaagg tcgccgctac gagtctggtc tgatatggcc atttgataaa
     3361 ctcctgaaaa caaaaaaccc gccaagtggc gggtctacag gaacaactct aagtttattt
     3421 tttctgttta aaattgtcca actcaggttc tttaacgctt tctgtcatgt aatccaatcc
     3481 accaaaaggt acttcaagca ttatactgct ttcataatca gccatggatt gctctgcttg
     3541 cgttggcttg ctttgctgtg agttgtcatt atttgatgtt gtcatcaact atgccttatt
     3601 tatcaaaaga aaagtaaccg ataatatgaa aaaaccgaaa gataacgcaa ttccttcaaa
     3661 aagataacga gcgtaaactg ccttttcttt atgaagactt tcgatcaatt caacagcttt
     3721 accatactga aacatatggt atctaagcgt tttaatttta tcaccgtaaa ataaaaaatc
     3781 atgctcttct tttcgcttat gaataggcat ttttggtata tctttcagtc cccaagtacg
     3841 ccataggaaa aaaaagcaca taacaataca aacaaagcac agatatgcac aaagtataga
     3901 taatgttaaa aaccaattac tctttagccc acttttgaaa tcaacagtcc caaatacagc
     3961 cagtagagca gaggtttcta cagctaaaat agcaagaagg tagttaactt tgtcctcaag
     4021 acgacgatat gttgattttg cttcctcata tctatcttta taatatgacg aaataaaatc
     4081 agcgcctata gtctcaattg ccttttgttc ttctgttgta tctattgacg aacgcttgct
     4141 catttctcaa tcaaccaaca actttcttac acgggaaaga gccaataatt ttcggagcat
     4201 ccatgctgtt ctgcaacaac tggacgttca ggaaacgcgt tttagtgcca ggtcgatgga
     4261 tatactcgaa cccataattg ttgccatctt tggcaggcat caggtccatt ttgatctgca
     4321 tgcctgtacc gtctttaccc aacattttta ctttttggga cgttactgtt tcgccattga
     4381 ttttaaataa tgaatcaggg atcgcatcca tacgaaagtt gccgcattgc atcgttatgg
     4441 tgtttgcact tacaccaaca gacactaatg ccagcaatga aaatatccat cgtttcattt
     4501 ctataatcct ctctgaaaag cagtgctgga tggcatatcc tgcgcaccgc gcgcttcgca
     4561 catcatatcc atgtaccacg cctggcccct tgtatcgcca gtgtacataa tcccgcgcac
     4621 aatataaacg ccatccgttg cgatgctggc aggctgcgat gtggtgccgc ttagcgtaat
     4681 atttccgtcc gtgttctggt cggtgatctg cccaccagcc atagcgatat cgttgttcga
     4741 caacgcggtg cgatatacgg aagcctgatc cagttgaatg agcccattaa cccggatgtt
     4801 cggattaata agcgcgcgaa cgtttacgcc gttgccgatg gtctgctgcg gcatgccaat
     4861 aagcccggta gcgctgttga gcacaatcgc ttcgtgaaca tattcattat tcgccaccat
     4921 ctggcgctga ccgtctacga attgccatgt tgcgccacat tgcccggcta cgttatccat
     4981 tagatgccgc gtcatgccaa agagcacccg cccccggggg aatacagtag caggcatttc
     5041 aggcgtcagg ccttcggtcg cacctttggc ttcgaagtct ttcatcagcg cacggttcac
     5101 atcagcgacc gtgtaaccgg cagccagcgt ctgtgaggtt atactggtgg caaaagccag
     5161 atcagtatct gctgcctgaa tcaggacgta ggaatcaacc ggactgtctt ttcctgtaac
     5221 cgagtagcga atttcaccgc tgaaaatcag tccgtagttg cggccatcac tctggcccac
     5281 gtccgccgca tcaacctccc gcacgatccc gacgtcgctt gccgacacct ccggcgcgat
     5341 accgtcgtaa cccgcaatca gacgcacttt cgaaaactcc cgcccggtga ttcggttcac
     5401 agtatctgcc gagaggttat aaattttgat agtccctacc cgggacgcgc tgctgatgtt
     5461 gaaccagtcg atcgtaaagg tcactttaaa atcacttagc tcaattccct gaccgttccc
     5521 gtccacaagc tgcagctcga aatgtctcat ccagttctgt gacatgctta ctccgttgat
     5581 accagtaaat ggctgcgacc gcccaggtca gtttttgtgg ggtaatcctg tgtgttgtca
     5641 tcacagacca ccaccagctt aaaaccaagc cccatacagg cgtactgcgc cagcaggtca
     5701 gcaccagtga cgagaggaat accggagatt accggctccc ctctgtcgtt ctgcaggtcc
     5761 ataatccagt acagatcgcg ccatatgatg ctaatccgcc aggtaacacc acccaggacg
     5821 atgctgaact gctggttgtc cgctgtcagc ggaatttcct gaattgccat tagccgcccc
     5881 ccagtaatga cgccacgtta cccgtgatgc ttttcagcag tgaagtatct ggaggcttgg
     5941 tggttttgtt gccgctgttc tgtaccgccg aggtgctggc cccttccttc atgttggttt
     6001 tatccgcgac ggaaatctgc tgtgtccggg agataaggac ctccctcagg gtgaggacgg
     6061 cggacaggac gttttcggtt gtcttgtccg tcgtcacttc cagcgcccgg atcaacatgt
     6121 tgctgtacag tcgtttaccg gttaccacat cgaaggggat acggctttcc tgcagatcca
     6181 gtagctcctg atacgtctgc tgaggactca ggccgagcag gctggtagcc gtcaggttac
     6241 tggcaaaatc cagcaatgcg ccaccaccgg cgaaaccaac ctccatcacc acttctgacg
     6301 gttttttata ggcatgatca gcgacagcgg ccccgacctc taccggatgc tctgttattt
     6361 caagcatatc tgtatgcttc tctgaaataa caacacaggg aacaatcatt cctatttttc
     6421 tgctctgctg atgaaaaagt gtagagagaa tatccactaa cccaccctca cctgattact
     6481 tcgcatgacc tgagcatttg cagactgttg ccgacgtgca acctcattac cgacagcgtg
     6541 cggatctcca ccaccgtaaa tgtggtaggt attttgctgg ttaacctctg tcactttgcc
     6601 actaattccc gccacggcag ccttattaat cagctcccga gaatagatat ttcttccatt
     6661 ctcatgctgg ataatgctgc tcatcaatgc tgacatggtt tgcggatcgc tcatattcag
     6721 ggcagcccgg ggatccactc ccagtcgttg cgatacagcc ctgatatacg cagttgtgtt
     6781 gttattatca gacgcaggtg cccaggtaga gataattttc tccacactgt ttattccccg
     6841 tccggcgtac agcattaact gacgagcaag agcccgtaat ccatcaaaag cagtttcaaa
     6901 tctggcaaat cgcccgcccg ggcgttcaag agaagcccct gcctgaccag caaaattaag
     6961 gtttcccgga ttgttattcc gttctcctcg tttcgtagcc tgtgcatgtt gttccggctc
     7021 atcatcacca aaccagccgc gtaccgtccg gcccacactg cggggatcga atccccagtg
     7081 ctctttaatc cagtcggcag tactgttagc gctgtctgta accatcggca tcgctgacgg
     7141 attttcgctg ccctgattaa gtatctgttt gccgatgctg acggcatcag cccagcggcc
     7201 atctttgata gcgttgagca ggtcggcgat catgttcagc attttgctga attcgcccat
     7261 ctggtcgatg aagttgctga aatcccactt cagggaccat gatttggggt caatattgag
     7321 cagtttcgcc agcgctttca ccaggtcgtt aacggtcgtt ttaaggtcac gaaccatctt
     7381 cagcgcggca tcgacctccg gtttccactt gccccagtca atcaggctgt cgccgccttc
     7441 cttccaggtc tgatagtcct cccacaggag ggcaataccc gccgccagcg cggtaatgag
     7501 gccaatcggc gacatccaga acgtactgtt cagaatgcgc agcgcaatcg tcagtgcgcc
     7561 aaacagcgag atcaactccc gcgtttgctt atccagcgat tgccaccagg tgataaggcc
     7621 tgatgtcccc tcaatcagtc tgaagaacag ccgcccgata atatccccga gcgccagaat
     7681 gccttttatg gctttcgtca gggtctgctc gatacgaggg aagttgtcca ggatatggcg
     7741 gcgcagggtg tccagcgaac ccgcaagccc ccccgcaaga ttagagccga ttttgtcacg
     7801 ggccatgcct gccatcgcgc caaactcacg cagggaggtc ataaatttgt tggagcttct
     7861 ggccgcctcg tcagcattga agccgatagc tttcgccatt gcgctgtact gcccggagaa
     7921 gccacccaca ccccggcgca tcgccataag ggtattttcg tcaatgccca gcatctgcgc
     7981 atactggtta gcccggtaat acggcatgct gctgagtttc tgtccaacgc ccgtaaaaat
     8041 agcggccatg tcacgcatgt taccgctggc atcacgggtc tgtacgccca ggcgattcag
     8101 aaagccttct gcaccgggat tgttacgaat aaaccgggat aggctttcca gagaagatcg
     8161 cgcagcgtcc acgctgccgc caacctgcga aaccgcatag ccaatagact gaattccctg
     8221 gactgtcgcg ccggtgcgct gtgacgccca gtaaagatta tccaggccgg aggcgatctt
     8281 agccgtgaag gccaccacgg acaacgcagc tccttcaaca gccagcccca ttttgatgac
     8341 atttgcagtt gtaccggcga ggacagaacc gaactttttc gctcctgcat catccacact
     8401 gaagccaagc gagacgagga aatctttaat agtttcagcg ttcattatcc tctctccatt
     8461 tctcaatgcg tcgctggtta tccgctttta ccgccagatg gtcattcaag agagcaatgt
     8521 catacaaatc gacagagcca tctttaagtg ctgtataagg aattaacccg gcgtcaaccg
     8581 gattgagaag gtaggacagc ccgtccggca ggctgttaaa cgtcagcctt gttgcaggct
     8641 ctgcgtcgtg ctggtaaggg gtgtaggcaa aaaatttccc agcgaatcgg cgaccacccg
     8701 cgccaccagc tgcagcatga ccagcaagtc aatatcatca aacatcagtt cgccctgggt
     8761 aaataccggc acccatccgt ccatatgacg ccgcgatacc accgcaagac agggatgaat
     8821 aatcgcatcg gtgtcatctt cggtcaggga agacagttcc tcagcgatac gcgggagcat
     8881 ggtttcaaac accggtttta actgttcgaa tttcacggtg tcgattttgc cgtcagcagg
     8941 caaacgggag cgaatactcc cgaaatctga catcattcct gccagcaccg gcagaagttt
     9001 gcgggtcact ttcagctggt caaaaacgct gagttttgcc gtgcgatatt tcacgccttt
     9061 aatttcgaat tccatgcatt aaaactcccc gagaacctgg tcaatcttgc cgcagtcaaa
     9121 cacccacggc atcgtattac cggttttagc gttggcgtta tccggttgtt tctggaacgc
     9181 aacactgcgt gccgtgatga tgtcgccgct gaccttgttg cggatcacga taacgttatt
     9241 cccccatgtg gcagaagact ggctctgtgc gttatacgcc agcgacaatt ttttatttgt
     9301 cggtgatgtc ttcagaaggt taacggtaat cgtcccgctt ttatctgcat ggagactgtg
     9361 catcacttcg ccatcagcac cgatggtcat ggtgttttta ggaccgccca tcgcaaccac
     9421 aatcccctct tcagaacttg ccgaaccgta cccgaggtca atcgaacctg tcggcccggt
     9481 cagcgtcgca gtgacatcca taaaagaata ggtagacatt cacttcccct tagcgaacaa
     9541 cgttaatctg tacgtcagcg taatgaaccg cgcctgcaag ttttattgca gcctgaatca
     9601 ccggagcctt acgggcttca cgttctgatt gagcctgttc atccagcggc tgagcgtata
     9661 cgtaataacc tttgggcagt gtgtcacctg atgacaactg accaaggtcg cccccgttcc
     9721 atacgcccgg agcaatcagt ccattctgaa cggcctgatc cagtgatttt tcaacatttg
     9781 ataacagtcg ggtaataccg gcttcagtct ggggaacttt cgtggtgctg gtataaagca
     9841 ggttatagag gttggtctgc acataattct gtaaccagtc caggccgtgg cgttcatcaa
     9901 agaaatcgcc gttagccatc actccctgct ggaggatagc cgtatcattc tggtagtaca
     9961 cgaatacatt gcagtttttt gcatcaagtg ccgatgcctg gctgactgtc agtgtttcat
    10021 acccgatacc cggctcctgc ttaaacttga gcgtaatcgc ggtattactg ccattgaaat
    10081 taaccgtgaa tgcccggcca aatgcagata acgcagcgta tttattaccc gatgaatact
    10141 gaataaaact gcgtgaatat ccggcggttt tcagttttga tgccaaatca tcgctggatg
    10201 cagtctgcag gcatttctca tcgcttgtcg taatcgccag aatacggctt acagaagagg
    10261 attcgatcgc cgcagccact ttcagccagt ctgcatcctg aatatcttca tcgtctgcaa
    10321 tccccagccc ataccatgaa gtataatcaa gcatggcatt cacagcctgc tccagcgtct
    10381 caggcgtggc ctgttcgctg tctcccttcg ttttcaccca acgaccaaca aaaacctcct
    10441 gaggtttcgg tgattgagag aaaaacacct gcgcagcctt atattctggt gattccacgc
    10501 caaaatcttt tccaatatct tccgcggcag aataacggcg aatgcgctca cttaccggaa
    10561 tgattgtgga cgggccgaga atgagtaatg caccaaaatt tcgccctgat gctgcacgcg
    10621 gcgacatgat cacatcaaca ttaacaacgt ttgatacagg caagccctgt gccatagctt
    10681 aatctccgaa aaagatgact ggtgcatcca ccagcgattt aataccgtac tcgcgcacaa
    10741 ccttccggcg caggcgcacc gtcatatcgt agcggcggac ccattgctga ttaataagtt
    10801 cagggaaggg agtcagacct gtgtaatcgc caagagacag ccccagcgca ttcagtgctg
    10861 cattgttctg cggcacagat ataccgtcac gaaaccggga cgcatacacc atccccgccg
    10921 gtccataaaa cgaagccata cactcaatcg tttcatgccg ccagagctga gagccatcat
    10981 cggtctgtct ggtgaatgcc ggactgtcat cacctgacca tccgataacc ccaaacgcac
    11041 accagttcgt ttcaaccggt agcagtggcg gctgctcttt ctgccagcgc gggcgaacca
    11101 tcccggcaga cagaccggaa acgttacgta tccactggct taacagcctg tcgagcgctt
    11161 cgtcataatc cggatcgcca ctggttggta ttaaccatcc gcgctctgta ctggtgttat
    11221 tgctcaaccg gagttccccc atcaaacggc atcaactcac aatgcgcctg aacgaatccg
    11281 gccccataag ctgtatacgg gtcgacgaag gtcacacggt aatcacggcc ctgatacgtc
    11341 acgatatcgg catcacggcc agtctgtccc tgcgtcagtc gctcagtcgt cacaatcaga
    11401 attgcaccac tgattacctg cccggcctgc atacgacggt tttccagaga gcgatcaaca
    11461 gtaacgactc cggcaaactg ctttttaact tcgctgtcgc tgccgattcc gtcctcatcc
    11521 accgtttgca cacggcgtgt tacccacaaa ttgaagtcgc aaaaatcggg gtcaaaaagc
    11581 acatctgtta catcaagagt cggcatcttt atccctcact acatgggtaa tagctctgcg
    11641 atattgcccg gtgtcaatta atggtttcgc cagatcggtt ccgggagatt cgccagcaac
    11701 acgccgggca agttccagtg ttgccccctt gcgcccccga cgagcccggg cttcaacagt
    11761 actgtcagca agcggcgtaa agccggtaat ggtcatgtaa cgcctgacgc cattaacggc
    11821 cagcgttccg gcacggttga gtgcactttc tgctcccgca gcattaccat caagtgcagc
    11881 ctgcgccgcg gctttaagct gcggcaccgt ctgctcttct gccgatttaa cgccggggac
    11941 caggtgaggt cgtggcggga tgttctgctc tggtgagccg tattcgttga ggtaaccgat
    12001 gcccgcatta ccaaacggaa catcatcccg ttcgctgtct tccgaaggga tgccgaccag
    12061 cacatctttt ttggttaacg acctgagcgc atccagaatg gccttagcgt tatccaccct
    12121 cgttgttaca ccgcttttga aactcatagc tggcgaccgc ctgcaccgaa catcgtgatc
    12181 aactgataaa attcagcgcc atatcgggtg ttattccaga aacctgcatc aggattcagc
    12241 gtcgcgctgg tgtcatagct gacgcttacc ttatccacgg actttgagga ctgaacacca
    12301 ttggttgaac cgcccggacc accagccagc atcgctctgc tgtcagccgc ccagagcgtc
    12361 atgtagtgag caacgaacaa cccggcaaag tacggaaaca actttttgcc ggtgacattt
    12421 tcgctcagca gttcatcggc cagattcaga cggaacccga tttgggcgtc gggatatttt
    12481 gccgggtcag caaactgcgg gaagtcgcgg cgaaaatcac ttaccgctgg cagactttga
    12541 ttctttgaca tctttagccc cattaccgcc agtccgggcg gcagtaatct gcgcctgcag
    12601 gctgtcgttc tgctcctgca gtttgagcaa tgcatcttta agatcggcaa tcagcttatc
    12661 tttgtcggca atctgcgctt gcaggccgtc gataatgggt tgcagatcat cggtgtcgct
    12721 aatcacgctt tcggaaagct cagagtgcgc ctgggtgaac cagtgcgacg cgacctcttc
    12781 cggtacgtta tgccgtcccc ggccaaactc ctgttttgac tgatcgccga gcgtcagcgt
    12841 aaacggggtg tgaacatgga tggtaaccag cttttctttc gccatttcag tttccttcag
    12901 gcccctttcg gggccattct ggttatcaga taccgtccac ataggacaga gtttctttat
    12961 acactggctc gactgcaccc agcttgccgt agtaagtgac gatctgatac aggccgcgat
    13021 actgcaccgg cacgctctga agcggaacca gcgggtagcg gacgtatttt ttatcgttgg
    13081 tgtacgcaac catgcgatcc ttattcccca caccacggcc tttcagccat ttaaccgcgc
    13141 ggatattcag cggaacaccg ttctggtgat agctgatggt gttggtctga aggtacgtca
    13201 acagggactg gttacccgca gatgaaacga tgatgctgga caacagagca aactgttcag
    13261 gcgggatcag caaatcacgc gggaccacag agtaaccaga agcggcccac gcatcagaca
    13321 gcacctggtt aatgcttgcg cggatttcgt ccggtgttga ggttgcccac gttttggcag
    13381 cgttgttgac aggcacgccg tccagggtaa caaggccttt caggtttaat gcggaatcgc
    13441 caacatatac ctgttcatcg ttatccatct gccatttcag ttgcatcccg tcatacttct
    13501 gcgtatcaat cgggcggccg acctgctgag cagcctgcaa ttctatgacc gtccagccaa
    13561 gttccatccc ccacaggttc agcgggttac cggatttgcc ggtatccacg ttcacgccag
    13621 caatagcggt tgagtctttg cctacccagt ttttgccatt cggatttgca ccagtacccg
    13681 cagcggcgaa gctggtattc gtccagctgg aaatgtcatc tgcgatagag acatcttcac
    13741 gcaactgaat atcgcgggtc caggtgtacc ccaccagtgg caggttcagc gtctggtcga
    13801 gtcgctccag ctccccgatg agaaaggcac cagagctgtc aacggttgcc tgatcaaaag
    13861 taatcattcg tctgttcctt aaatcttcca ggaaatttct gcattgccgt tagcatcacc
    13921 ggcacctgtg aattcagcgt tggtcagcac cacatttttg ccactgactg acgtggacat
    13981 gaatccaccc agcggcactt tgatggattc atcagtggag acgacaacgt ataccgggtc
    14041 gccttttttg atagtgctgg catcaaaatc agaaccgaga ttaacggtca cgtagccacg
    14101 cttcatggcg tcgcccggga agttcttgcc tgttcccacc tggcgaacca tgtccggctg
    14161 cgacgtggtc ggataagggc gcacgtagat ccccttcacc ttgtctgcgg tatcaccatc
    14221 tgccagcggc acgaaaaaac cgtcatcatc gtatttacca gccagcccat aggcagcgaa
    14281 ggcgttatcg gatttaagga ccaccggttc gacggttaag tcctgcgggc gagagacagc
    14341 cccggcaata ccaacaggca tccggtacag aaatacatta ttcatttttt accctttacg
    14401 gtttgcccag aattcagcgt tttgtttgtt cagggaagcg atactggtca tgcccatgtt
    14461 taggcgctgt gcatcgccgg tggtggcgcg ggtgtttcgc cctttggcaa tctcagacac
    14521 ggcattaaac gccatgtcga ccgattgttt cggcaatttg cggatatccg catcaccgac
    14581 tatctggcga accagcgttt tgtcagcgga agccagaacc tcgcgtttga acgcggtcgg
    14641 tttcatctta cggctcagat cgatacccgg aacaataact tcggcacgcc aggctgagtc
    14701 accagtaatc gtggtttcct cttcatcgtc ctcgccgtca ccggtcggat tatcgtcagg
    14761 cttattatcg ttatcgcccg tggcatttcc ttccagctta gccagcaggg ctttcagtaa
    14821 tgttttgagg tcatcatcac tgtcgccggt tgggcctccc cccatctctg gtgctttgtc
    14881 cggtagtggt tgctgcgggg acaggttgat attgagatta acgccctgcg gcaaatcccc
    14941 ctcatctcct gtaaccgatg cgggagccga ctccaccagt tcgttcatgg tgtcggcatc
    15001 tcctgtcttg atggccgcac gcatgcggtt ccaccagttt ttcttttgat ttgccattgt
    15061 gtctctgtct ccaattgcac aacgatttcc ggctctgcct ttagggacaa gagccacatg
    15121 gtttccggta atatcgacct gctcagcttt acctggctcg gtctgctcgt actccgcgtc
    15181 atagccgcac gacacttcgc gcaggccatc ttcgataagc tgaatggcgt tttcgtcttt
    15241 gacgataagg tcagccagca tcaaatcaga ctgctcaccc gtcccgcgcc ggacattctg
    15301 gaggtgcccg acagcaagct ctttccagtt ctcgggattt accagccgca cattcccgtt
    15361 ttcatcttca ggatgcagaa tcgtgatgct catcccttcg aatgaggcaa gcgtggccgg
    15421 atggaatacc tgctcaggag aacgcgtgac gactatttca ccgaacttat cgggtttcag
    15481 ttttggcagg tcatcagcac catagagctg cttacctgtt cgtcctatcg gcacgtctct
    15541 gcacagcaac gagccgtcag ccagctgata gcgggtttcc cccagccggg tattgaaaaa
    15601 atatttcatg tgttacctgc gattcaggcg ggataagatt gggaggtggg aaaaacgatt
    15661 tctttataac agcgacaatt cgggagctcg ccagcgtgac ctgtcatgcc gtcaagcgtt
    15721 ggaggtttgc cccattcgac aaatttacct tccatttccc gatgagaatg cctgacgtca
    15781 ccatcttcgg ctgtacgcca gatataacca ttcgaaccaa ttgacagcgc acgcgcctga
    15841 tccagcgcgc cggttgcacg tccaagttca gtacgggcaa tcaggtcagc tctggacttt
    15901 gctatatcac ccgatgcggc tatttcttta gcaaaatatt ctgctctccc accggtcaca
    15961 acagcttctg tcgcccgatt ctggatgtcg tacaccctgt cagccgcctc gagggggagc
    16021 gatttgatgt acttgacctg ttcggcgatg atggattgca tcacctggcc cacaggagcg
    16081 ctttccacaa gattgcggag ctcgcgactg atgttcttgc tgtgttgccg ccaaactttc
    16141 tcgttctgcc gggttaggtc cgcagtaaag ttttccgcga cctttgtcgc ccagggggtg
    16201 atgatttcac tgtagcgttc cagcgcctca ataatttccg tgatactgtc atttgaacca
    16261 tcgtagcgac catttacgat gtctccgacc gcccgcgcta tcctgcgtag gctggttcga
    16321 tagcggattt ccgcctgacg gttcctgcgg ttcgtcatca gattcgccga tgccgggcgg
    16381 cgcttcatct tcggcattct cgatgtcctc gtcggtaatg gatgccccga tgccggttac
    16441 gtcagaattt tcgcgcaaat cagtcatagc ggctttcagt gtcatcagac catcacccag
    16501 cgccgtactg attgcgttgg tagtgtttaa cgccaccgtt gagcgatcga catcagacat
    16561 ttgccagagc gggttaaact caaacgtgaa atcgtccggc agcggcttgc caagctccga
    16621 acgatgcatg atgtccagta tccgccgcac cggaagacgt aaacgcctct cctgtaacga
    16681 gcttacccgg tcgtaatagt tggcaaggtc tgcatcgccg gtagaaaatc ctttcgggga
    16741 ctgtccgaac aaccgcacca gtgggatacc aacagcgcca ctaatctgtt ctgcaaactg
    16801 cgaaaggatg tcatccagac cactgaagct gtactgatgc gtttcaaact tatcccgcga
    16861 gtccatgagt gtcatacctt cattgctctg gaactgtcga atcaggtcga tattcttcag
    16921 caacgcttca tacgcaggac caccaagtgc gataagctcg cgtagcttct ccacgctgta
    16981 ggtgcgcaga tgcgccttgt agaccagctg cgccgcgccg acagtagcgc tgtcgaacgc
    17041 ggtaagacga tcccagatac gctctacaac cgacattccc cattcgttct cggtcatctt
    17101 ctgctgaaat ggcagcgtga cgccatcaaa gcgaatcagg cgactgtgat gaatgcgcca
    17161 ggcaggaatt cccgttgctg tggtcaccac atcgtaaaac tcaggtttac ccaggtccgg
    17221 ccccatatct ttaatgcggc gggtcagtac cgggtcgatc atccagcggt cgagcgggag
    17281 aatcccctta aacttgccct taccgatggt ttcgggtcgc agcggggtca ttggtgcctg
    17341 cccctcaatc atgatgaaac ccaccgcgcc gccgtagagg cgcgaccatt tcagcacgtc
    17401 attcagcgca tcccagattt gcaactcatc cagttgtgat tcgagaatgc cacgatcttt
    17461 tgcatcaatt tccgacgtga tgcgaatgcc tttgcgggtc atatcatccg ggatagcatc
    17521 gactgcttcg ccgatgatcc aggatgaacg ataggaccat tccaccagca tgcggttacg
    17581 actggtgaaa ttagcccggt aggtggatgc tgagtgctgg ttaggtgtct gcatccctac
    17641 gcgggcaata aaattctcat aaccatcagc tgtggcctgc gcagttcgcc gcagggcttg
    17701 tttgtttcgt gccatcaggc ctgtctccct agcagctccc agatgttcag ggctgaattc
    17761 attggggcat agttgatcat caccgagtcg gcaaggtttg gcgatcgggt tccatcaggc
    17821 tgtttatcaa taacgatttt tcccacacca ttaatggaat aggtcggctg cgaaagctcg
    17881 atgatgagtt tatctttgag tgccatgcta ctgctgattg agatgatttc gtccgggttg
    17941 taagccatac cttcaaccac ggcgcgccag gtattctgaa aaagtttacg taaccgccac
    18001 cagctctggg ctttggcgtt agcgaagaag tccttgttca gacgtgcggc ttgcccgttg
    18061 tccccgcgaa cagcttcatc atccggatca aataccgcgc cactacctcg aaacggtgtg
    18121 gcaagtattg acggtcgacg cgcagcgtta cgcagttcgt tgatagcgcg tgcatcgccg
    18181 cgaacgccag cgcccagccc gtcctcgtca aagcgaaact cttcgaggtt gtcctgttcg
    18241 caaaagccga aaaccttctc gacggactga taaatgtcgc tgcccacacc ggaccattcc
    18301 cgcacatttt ccaggaggaa gccatgacgg gtggaaaagg catttttgtc cctgccttcg
    18361 tcggcgacat ccatcgcgcc aagtcgtttg cctgttggct ggatacccag tttgatatgc
    18421 gcatcaacgg cagcctgtac ccattcggat ggaatcagaa cgccttccgc tgatgcgctg
    18481 tagttcagat caagttcctg tgccaccacc accggattat cgattttctc gcattccctg
    18541 cgataccact cttcatcctt gcgaggatca tcccgccagt ggaatgtgaa taccggtatc
    18601 ttcccgccat gacgcttctg agcgaacggg ttagccatgc cgttaactga actcaggtcg
    18661 atacggcaac gcgtcgtttg tgacaacgcc gcatcaatca gcagaggacg ctgaaggaat
    18721 gcagcctcat caaccagata aagcgtggta cggtcaccac gaccaatatt atcgccagcc
    18781 tcgcctttga taacggcacc agtttcagga aactcaacac gcatatatgg cgcgtgcttc
    18841 ttctcgctcc acgaaccgcg aaactctaca ggtagcgttt ccacgaactt gcgtgccttc
    18901 cagaacaatg ctttcgggtc accggtgctg tcgacgtatt cctctttacg ggagccgaaa
    18961 ccgataacca tttctttgtt gaagagacaa agcgagcagg ccagtccgat cgcggtccaa
    19021 ctgagcccca tttcacggga tttttcggta ataccattct cccgattgcc ccagcgttcc
    19081 ataatccagt ggatccactc ctcctgctta gggaagagta aaaacggaat ggtcaccggc
    19141 aggccataat caatattacg cgggtccgtt gtcatgcccc agtcgatgat gaactgagcc
    19201 ggattggttc ggtaaaactg cttcaatacg ggcaatattt caggattctg gcgaatgcgc
    19261 tgtaggcgtt ccatccgcca ttcaaaaacc atctggtaat caggatgttt aaaatcgaag
    19321 gggaatggta acggcatact tagcccatca tttttctata cgcctctgca gcctgctccg
    19381 gcgttaagtt ggtaatttct gttctgacgg gtcctccatc agcgccagtc acttcatttt
    19441 tgacgttgtc tttaaacgcc tgaacagaaa catgacgccc aagcaactca aggtttttaa
    19501 ccttatcagg ccacttaatc tttttaagga tcccgaccat ttctctgtca tctcctcgcc
    19561 cctcaaacat ttcagcgagg ttaaatccac tcaggtaccg acgccacgat tccggccacg
    19621 cagacagagg cttaatactt aaatcgtcct ccaggatgtc agccacatcg agcctgtcga
    19681 tctcaaccag tcgcatcagc acataattag catcaatgcc cagttgatca atacgctcct
    19741 gctttagctc gttaatacgg gcgcgtatct caggtttacc gtatagttca gcccccgtaa
    19801 catgtgctcg cctggagacg tagcctgcgc gaatagctgc ttgtgtagca ttcagatcga
    19861 caagaaactc gcgacaaaac acctcgtgtt ttgctttcag cttcttagtc attttatttt
    19921 ccagttatca ggtcattatc gaagcccctc ctggaagagc ttctgtaatg ctattaccgg
    19981 gactgttcta tttgtcggac accagccaac tggttattcg ccttctcgat ggctgccaac
    20041 agtgggttaa tccacagaac agcctggcaa tatgtcaacg ttctggtggt agtggcacga
    20101 tcaccggctg agtcaatgtc cccggaatcg gcgtgcaatg cgctggcgcg taaacggttc
    20161 gcgtagttga gcaacccgac agcaatatca acaggaacag ggaaatcaca gttcttttca
    20221 cggagcagaa cctcacggta tttgatgact gtcttctcgt gaccgatgtc gaccagagaa
    20281 tttaatcggc ttgcgttttc tgctatctgg ttaaaacgat tgaagtttaa tgcctgatta
    20341 gctatcactt tcccttgata ttcagcttca ccttccgctt tatcagcccg caacttttct
    20401 gcctgatact tgctgtggta atggtttgca gaccagacga gcgccccaaa ggcagagaag
    20461 aaaaatgccg cgatgacaat cttataagtc agcttcattt accaccccac cagcatcttt
    20521 aaacctggaa atcaggtcac cgattttatg ttcatactga ccgtaacctg caccaggtaa
    20581 cgacgcccag atattgctgc aacggtcgat tgcctgacga atatcgccac ggtcaatcat
    20641 cggtaaagcg ccacgctctt taatctgctg cagcgccaca gcgtcctgac tttctggcga
    20701 aaaatctttc aggccaagct gcttgcggta ggcatcccac cagcgtgaaa gaagctgata
    20761 ccggcctgcg gctgttgatt taagcttcgg gtttagcgtg acaagtttgc gagggtgatc
    20821 ggagtaatca gtgaacaact cacctccgac gataacgtca taaccgtgat tacgtgtcgg
    20881 ttgtcgcccg ttatccgttc cttctgacca tgccaccata tccaggaaag ctttgcgttg
    20941 ggaatttagt gtctgcatga attactcctt agagccacca aacttgttac cgattactcg
    21001 cattgcagcc ccacgaatag catcgacacc gatcagccca acaccaccac caatggcaac
    21061 agaaagagat ttaggccatc caacatactc aagagcggat gcaaaggtca gcgtcagagc
    21121 accacagagc aaaatctcaa gcgtttttcg tttccagccg ccgccaccgc caaaataggc
    21181 aatgcgcaag ccagccataa caattgacat aaccactgcg cccaacggcg tatctccacg
    21241 ccaccaactt tgtaagagtt ccagtaagtc aggccaggaa tgaggagcat tgtgcatttt
    21301 catacttccc acctccgcca ttacggggtg ttgttgaaag gcgggccctg cgtatacgcc
    21361 cgtaggattg ggttatgagc cgtccttcgg tgggccctga atacaaaaaa agttcgccat
    21421 agcgaacctt gttaaatttg ttaaaaaagg aagttattta acataatgta cgttatagga
    21481 accacacgat ccccgctcgc aatagatttg cgatgaaagg cctatttaat caacttaagt
    21541 ggtccagaat gacgaaattc gagtgaataa aaggtgcata aaaaagggca aaaactgcat
    21601 agcgtttttt cgcagcgaaa gccctgtttt attaactttt ccccagaatg gggcaataaa
    21661 aaaggccccc ttgcggaagc ctcttcgtga tgaactgttt tcaacagata tcaggctaat
    21721 cggctttttg gaattccaca actatttttt atcttacctt gatgcctgat gccgtcagat
    21781 gtttacgaca gtcctgaata ccttcgttat aacccgcatc gtagaagcta tccggctttg
    21841 ccttcagcgc aggaagcgta acgctggtag ctcttcgttc ggccagaaca cctgcctgaa
    21901 agagtgtcca catcaactga actgctgaat cacagtaagt aacatcttca ctgtacaatt
    21961 ctcgttcaat cctgatgcca agttgagcct tcattgatgc gccaatatta cctttgacct
    22021 gcaacgacag gtctgggtac ctctgttcca tgaagctttc aaatctgttg cgaacgctga
    22081 tcagagccat ctggttccct tacgtgattg attgctggat attatgccgc cgagcagctc
    22141 ctacgcaaag tcctgatagc ctgaggcttt gactgaaccg actgatccat ttcgagcacc
    22201 acatccagca tggctaagca accgtcaata aatccctctg ccgcctgaag cctcttcaga
    22261 acgtgggtgt gagatacgcc gagcttctca cccatcgccc gcacgggcat ggcctggacg
    22321 taatgccact caaggagcgt gcacagatac ggatcttttt tcttcaggca gctcatagcg
    22381 gagttgatga taagaccgtc attgtcactg catttcagcc ttccagactg cgtcgcgggt
    22441 agcagattct taaacccggc tgcaacagga ggaaagtaga cgctaccacc ttcactcgct
    22501 gcccagccac cccagcgctc taaaacctgt tgaatattac gcatagctct gacctctacg
    22561 ttttgctacg aaaaattttt ctgagaacta cgtctgttta tccgtgggtg tggataacgc
    22621 agttccgatc cgcatattgc atgtatcaac atgcaaaaag ttgctaattc ggagtggaac
    22681 cacctgcccc cacctggaac caccttttcc taacctttcc ctcaatcgac ttatatatat
    22741 atggggtttc tagtagaaag gcggttccag tggttccagt agttccgccc cgcttgccac
    22801 aaggtctgca aggtggaacc accttctctt ctaggtggtt ccgcgtggtt ccagcggttc
    22861 ccaaaccttg cattttttcc ccccaattcg cctttgggca cgcttatacc cgcaattttg
    22921 caaaacatta ctaattcgca tttcttcgcg ttttccgatg cggtcaggat ttagcccaat
    22981 cgcatcacgc aaaacatcac ttgcgcgtaa aaattcgcaa tttcgcggaa gttcattagt
    23041 catcaggtcg ggcgtgtcga gccatttctc taccgtttca agccaagcat ccttgatggt
    23101 gtactgttca tggacactcg ctgccagttg ttcagcctca cggaactgga taccccccag
    23161 acgctgaaac acctcacgag cctcagccca aagtaaaagg agatctcttt ttatcgcttt
    23221 cacgtcgact ttcgacactt ccacggggag ccagcgacgg ttaccagtct tgtccgcaag
    23281 gaattcgtcc tcattagtgg taccaacgaa caccaggcga cgaggaaact gggtggcgaa
    23341 ctcccggtat ttcgggatcc agttttcgtg agtacgcgtc acaaacgctt tgattgactc
    23401 cagctctttg gtattaagcc cgcgcagttc accaatctct gccaccagcc gaccacgcat
    23461 ttttcgagcg agatcatcgt ctttttcggc aaaagagatt tcagtaaaaa acgcaggatc
    23521 agggctcagt gcctccactc cggaagactt accgcagccc tgcggaccaa cgaggatcgg
    23581 caccatatcc gctttgatgc ctggctccag tactcgcccc gccaacgcgg tccacatgta
    23641 catagacacc gcacgggtat aaggcgtgtc ggcggtaccg aagtgcgtat ggtagaaaca
    23701 ttcgatgcgc ggcacgccat cccactccag tccgttcagc caggtgatcg ctgaatcgaa
    23761 tggttgttca tcggctgcaa gtaacaccac atcgcgaata agttcacggc caacaggttt
    23821 aaatccccgc ttttccatcg tgatgcgcag gcgcgcataa tccgcatcgg tgaatgcccg
    23881 ccattgtccg gatcctgccg gggcaaacat gatttcgtcg cggaactggt caaagcgaat
    23941 atcgatgtcc acaaaatcag agcgtactac tgctttggct gcattgctga tggttgcctc
    24001 gatacgcccc catttatcac gctcgaaagc aggcagcggt aaaggttccg ccacttcgat
    24061 gctggtcaga tcttcgaaat cgtcgttgcg gatcccgatg gcattcagga aatctccgtc
    24121 atcacgatgc gcgcaactgg catgcaggca tttaaaatgc ccctgctcaa agcctgcggt
    24181 tcccgcagga aaataaactg tgcttgttgg atcgcctccg ctactgtggc cgtcttcaaa
    24241 cggacagcgg atatatcgtt caccgtttgc gccatccagc agcgtccagc cattggcatc
    24301 aagatattca gctgtatcat ccgtcgcgcc gggcgtgaat gctgaacggt cgcgcatctt
    24361 cgtgttgccc gcttcggtgg ttaccgacac agggagttgt tcagccaggc gctgccacag
    24421 cgtttcaagc tgctcacctg taatagccgg aggttcatcc ggcaaaccac cgtcccattc
    24481 aatacgcgcg ccgctgctgt gcgtaccaca ggcaacgaac tgctgcccgt tcgccagcaa
    24541 ctcgataatg cccatatccc ccgccaggcg atggatacgc ttacggaaat caccatcaac
    24601 ggccagcaga tacagacact tattactgtt tgctcgccag cgtcgcggcg gcgactcacc
    24661 cagaagttgc acaagtgttt tgcgaatatc tgcctgaatg tcttcatctt cgctatcgca
    24721 gtccagcgcc agccagccat ggcctgtacg cacgcagatg ccataatccg gttcattcga
    24781 ccagcgggca aaatcatgct cagtaacaac atgcccggtc cattgagcaa tacctgtgac
    24841 ctggcggtcc cggttatagc gactcggcgt cttacccaac gctttcaatt tactatcagg
    24901 ggatatggtc gcactggggt tacatacaac tgggagaaga tgagcagtcc gccccaatac
    24961 caggtcgaaa tgaaaccact catcaggcgt agctccccat ggtttgctat cggacatggg
    25021 ttacaccttt tgtctagctg tcacttcctg aaaaagtttt tctatcgcac gtactgtaga
    25081 aaagcgaggg tcagaatgaa ccccggtaag aagccgacta atagaagact gcttcacgcc
    25141 tgcaatttcg gcaatttggt tttgggtata ccctgaatca atcaagcttt tgaccatctc
    25201 ttgtggtgtt agtccggaca tatttaagtc tccactttcg tctatagcgc aaatttatac
    25261 gaaaacggat tactcagcaa tacagcaatt ctatgtcatc ctagattaaa atatccgata
    25321 acgcataatc accgtccctg tatacaacat ggatgaatcc aaatgattga tactaccgat
    25381 atcctttctc aaaacatcaa gtacctgatg gacaaagcca agataagctc gataactgag
    25441 ctggcacgtc ggttacagct gaaccaacca acgctacaca ggctggtatc aggagaggtg
    25501 aaagatccga aatacgcaac gttaaagcag atcgccgact acttccacgt gtcaccaata
    25561 gacttggcag aaaggagcct tcaagaaatg gagaaagagg acgcgacagg tataaaaacg
    25621 tatatttctc ttaggtttaa taaagtacct gttttgggaa acactcagct aggcgttgga
    25681 ggactttgga gcgacaccca atattcagta ggtagcagtg atggtttcat atactggccg
    25741 acaaaggatg aagatgcata cgccctgaaa tgtgttggtg attcaatgat gcctagaatc
    25801 aaagaggggg agttcgtaat cgtcgaaccc aaccacgact acactcctgg tgatgaagta
    25861 cttgtggtta cccgggaagg tgaggtcatg gttaaaacct tcttgttcga acgcgatggc
    25921 ctgttccatc taatgtctgt aaatgaagat catccaccag tcagagtacc acgcgaaaac
    25981 attgaaaaaa tccattacgt ggcggggatc gccaaatccg cgctacgtat gtactaacct
    26041 ttacagtctg ataacttact tctgatcaaa ccgctctcca caagggcggt tttcttattt
    26101 gcaaaataat tcattttcgt attgacacaa aatcccataa cgcatataaa tacgatatcg
    26161 cataattaat gcgtttttta atactggctc tttaacaaac tgaaccgcgt gacaggtaag
    26221 ccgcagtact cctggcaaaa cgaaatagca cccgatggga tcgaggtaag cgccgagtcc
    26281 gtatgcgtac ggtaagcgta gaggacaaca ccgcgacaaa ctgataagtc acgcaagttg
    26341 aaacgccccg acgatggggc gcttagttcc ttttggggtg gggtgaagat cagtccattg
    26401 agtcaatccg aagttcagca tctaccacta caccaccacc aaagagaact gctaatcatc
    26461 tggcaggttg taacggtgca ggagttttgc cactgacttc tatatttcgg ggaaatttat
    26521 attcagcgga gcatttatga cacttgagaa ttttataagc ggcattagaa gtatggtatt
    26581 gaagtataga cttcataccc gactcatagc atgtagtaca aagataatga ggcttgaact
    26641 caggatcgtc agtaggcttc agtacataga ccaccgtatt ggtaatcggg tggtacattt
    26701 catagttcat tttctcacta tcccaatcct gtttcttttc cagaagggat tcgagttcaa
    26761 caatgcgctg tttagcttcg ttcaaaagct ccgttagtgc gaattgtcca gtacgcgcat
    26821 caattagttt atcaagtaga tcgatagttt tacttttaac attgtaatcc acctgcaacg
    26881 cattaacttc ttttgctaaa tcaacagcac ctttcaaagc accaccagca ctagtggccg
    26941 cctcagttat acgccgtaca agtccttttt cttcagacat cttaattctc tctaaaacta
    27001 taggggtaaa gagatattag ccgaattctc gctgtagggg tacagagaga gccatctcgt
    27061 ctgacgtggt taaaagcagg cattcgctga catccaagta attgctgtgt gtagcctttg
    27121 cccgcctcaa gtgacgggct tttttatgtc tgaaagcgca ctcgcaacag cgtgctcccc
    27181 gatatgaaaa aaggaataca accgatgaaa cctgaatacc tccatcgact gacggggcgc
    27241 gatgttctcc gttatcgccg taaaaacttc gatttgatga ccggtctggc cgttgccact
    27301 gcgctcggtc tgatcatcac tttcattctc cttgtagcga ggaccacagt atgagtttag
    27361 aaaccagtct cgaacttaat aatcaacttc tggcacaaca taatgcgctg cttgaacgtc
    27421 ttatccgcac aatggcatca ggcattgtta tgcatcctga cacgatttca cgagtgcagg
    27481 aatgtcggga tacagcaact gaaactgaaa atatgtcagc ggcaatgaca ctggatgatc
    27541 tggagttcag cgacgttatc gcactggctg gtttctaccc ggtagccacc cctatcacag
    27601 aagacatgct gcaacgtgct gttgcctacc gtgatgctga aggcgataaa cgagtagttc
    27661 agattgatgc tctcgacagc gcattgcagg gcgtcaaacg agccagggcg ctgcttaaac
    27721 ctgctctcct ggacctgtcc cgtaacatcc ttaagttctg ggacgacctg ccaaccatcg
    27781 gcgagcgacg tgtttttgcc gagcagctac ttgatgcacc tgcggatggg cgtgatgaag
    27841 ttaagccgaa aaaggccagt aacaaagatg gagaacgcac ggggccgttt tacgtcaaaa
    27901 atgtatccgg cacagcagcc agtgaactcc acaccttacg caagttgaac gagatgctga
    27961 aaaaaggcca tatcgagatc aaccgtgttg agtaccttca gctgcaagaa gaatttgcac
    28021 gcagagacgc agcaaattcc agccagaatc atgacgccaa agatgaccat acaattgatt
    28081 tcgcggcact acgcaaacaa gctgaagggt tgatcctcca gttagcaaaa gggggttacc
    28141 gggcagaagc tattgcaatt ctggaaaaac agggagccag gaaacttggt gaagtaacgg
    28201 atgaaaatct cgctgaagta atcaccctgg ctgaaaaagc actggagggt taatcatgcc
    28261 agacgttcat gcacaacttt ctccatcatc agcgcatcga tggatgcgct gcccaggaag
    28321 tctggcgctg gaggccacac aaccggataa agaaacaact tttgcaatcg agggcactgc
    28381 agcgcacgcg cttgctgaaa aagttctacg aaacaggcaa agctacccgg aacactacgc
    28441 cggatgcaat gtttctatgt ttctcggctc ataccccctt cgcgaaaatc ctaatgatac
    28501 atctggccca caggtggatg atgaaatggt cgaagccgtt ggccggtatg ttgatacggt
    28561 ctggactctt gcacagaata atgaactatt ggttgaacaa cgtgttgatt tctcacatat
    28621 aacgggtgtg gaagaatctt tcggaactgc cgacggcata atcattgctg gtagcgaatt
    28681 acaaatccac gacctgaaat atggcaaagg cgtccgcatt gatgcagaac aaaatgagca
    28741 actacaactg tatgctctgg gtgcgctcga acaattcagc atgctgtatg actttgagac
    28801 tgtaagatta tttattcacc agccaaggct caaccacgtt tcagaatggt cgttaaccgt
    28861 acaggaactt cagtctttcg gtgaacgggc actggaggcc gcaaccagtg cgatccttgt
    28921 tctcaatatt gctgaatgcg aaggcattga gacactaccg ctggaaaact tcatacctgg
    28981 agaaaaacag tgccgcttct gtaaagcaaa agctatttgc actgcccaga aaatgcagca
    29041 tttacaaaca gcggccagcg attttgaaga tctgacaaag cctgtcagcg aaataatctc
    29101 caatgccagc gcacgtgtac ctctgttaac cattgagcag cttgcggaga tctatagcca
    29161 ggccgacttt attgaatcct ggctaaaggc agtacgagac cgggttcaca atgaactcaa
    29221 tgccggacat ccggtaccgg ggtttaaact ggtaacagga aaacaaggta accgggcctg
    29281 gagtgatgaa gaggcagctc gcgcacttct gaaggaccag ttcaggtaca aaactgaaga
    29341 agtattcgac tttaaactta ttagccccac aaaagccgaa aaacttatca aaaaggccag
    29401 tccgcgccgt tggtcaaaag tcgaggcact gataacacga gctgatggta aacccaccat
    29461 cgttcccgag tcagacccac gccccgcact caatatcaac cctgtaaatg atttcgacga
    29521 cgtatccgac gatacgctaa ccgcagacct catctgattt aaggaaatac ccatgaaact
    29581 gaagctgaac aatgttcgtc tggccttccc gtctctgttt gaagctaaaa ctgtaaacgg
    29641 cgaaggcgat ccacgtttct ccgcagtatt tttaatgtca cccaaacacc cacaactgga
    29701 agaaatccgt aaagctatga agcaggtagc gaaggaaaaa tggggagaga agtgggagcc
    29761 catttataac caactggaga aaaaactcaa tctgtgcctg catgatggtg atgaaaaagc
    29821 agagtatgaa ggcttccccg gcaatttctt cctgaatgct gctaacaaag cgcgcccagc
    29881 tgttcttgat cgcgatcgtt cgccacttat tcaggctgat ggacgtccct atgcagggtg
    29941 ctatgtaaac gccgttatcg atatctgggc acaggacaat aatttcggta aacgcattaa
    30001 tgcctcactc ggcggagtcc agttcctgcg agacggcgat gcattcgctg gcggcggagt
    30061 ggcaagcgct gacgatttcg acgatatcag cgaaggtgct gatgctgaag cactgattta
    30121 accctacttc atagacgccc ggttaaatac cgggctgctg attaggcgta taatcactca
    30181 ggaatcttaa tcttcctcaa ttctttattt agttcacaga tcttcttctt agacgggaaa
    30241 gtatcataca tccaaaaacc aacagtctct aaaactattg tcataaccat atacacaaga
    30301 taatttaaaa ggccttcatt ttgccctatg gaaataatgc agataat
//
LOCUS       DAZFWP020000047        30276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFWP020000047 DAZFWP020000000
VERSION     DAZFWP020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.047"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            113..1696
                     /gene="yegH"
                     /locus_tag="ACHK0Z_RS09820"
                     /old_locus_tag="pgaptmp_001964"
     CDS             113..1696
                     /gene="yegH"
                     /locus_tag="ACHK0Z_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4501993.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2147..4000)
                     /gene="asmA"
                     /locus_tag="ACHK0Z_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             complement(2147..4000)
                     /gene="asmA"
                     /locus_tag="ACHK0Z_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU4501994.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTYVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4022..4603)
                     /gene="dcd"
                     /locus_tag="ACHK0Z_RS09830"
                     /old_locus_tag="pgaptmp_001966"
     CDS             complement(4022..4603)
                     /gene="dcd"
                     /locus_tag="ACHK0Z_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4501995.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4695..5336)
                     /gene="udk"
                     /locus_tag="ACHK0Z_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             complement(4695..5336)
                     /gene="udk"
                     /locus_tag="ACHK0Z_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU4501996.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5654..8971
                     /gene="dgcE"
                     /locus_tag="ACHK0Z_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             5654..8971
                     /gene="dgcE"
                     /locus_tag="ACHK0Z_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4501997.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9010..9867)
                     /gene="alkA"
                     /locus_tag="ACHK0Z_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             complement(9010..9867)
                     /gene="alkA"
                     /locus_tag="ACHK0Z_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU4501998.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            10001..11353
                     /gene="yegD"
                     /locus_tag="ACHK0Z_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             10001..11353
                     /gene="yegD"
                     /locus_tag="ACHK0Z_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4501999.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11365..13305)
                     /gene="yegI"
                     /locus_tag="ACHK0Z_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             complement(11365..13305)
                     /gene="yegI"
                     /locus_tag="ACHK0Z_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU4502000.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13302..14063)
                     /gene="pphC"
                     /locus_tag="ACHK0Z_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     CDS             complement(13302..14063)
                     /gene="pphC"
                     /locus_tag="ACHK0Z_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU4502001.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14060..14719)
                     /gene="yegL"
                     /locus_tag="ACHK0Z_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             complement(14060..14719)
                     /gene="yegL"
                     /locus_tag="ACHK0Z_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4502002.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14940..14999)
                     /gene="ibsA"
                     /locus_tag="ACHK0Z_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             complement(14940..14999)
                     /gene="ibsA"
                     /locus_tag="ACHK0Z_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU4502003.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15272..15328)
                     /locus_tag="ACHK0Z_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             complement(15272..15328)
                     /locus_tag="ACHK0Z_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4502004.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15600..15656)
                     /locus_tag="ACHK0Z_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             complement(15600..15656)
                     /locus_tag="ACHK0Z_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4502005.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15935..17182
                     /gene="mdtA"
                     /locus_tag="ACHK0Z_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             15935..17182
                     /gene="mdtA"
                     /locus_tag="ACHK0Z_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU4502006.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLSSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17182..20304
                     /gene="mdtB"
                     /locus_tag="ACHK0Z_RS09890"
                     /old_locus_tag="pgaptmp_001978"
     CDS             17182..20304
                     /gene="mdtB"
                     /locus_tag="ACHK0Z_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU4502007.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SCFARHEEEA"
     gene            20305..23382
                     /gene="mdtC"
                     /locus_tag="ACHK0Z_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             20305..23382
                     /gene="mdtC"
                     /locus_tag="ACHK0Z_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU4502008.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTADEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRSSLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRMRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23383..24798
                     /gene="mdtD"
                     /locus_tag="ACHK0Z_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             23383..24798
                     /gene="mdtD"
                     /locus_tag="ACHK0Z_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502009.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24795..26198
                     /gene="baeS"
                     /locus_tag="ACHK0Z_RS09905"
                     /old_locus_tag="pgaptmp_001981"
     CDS             24795..26198
                     /gene="baeS"
                     /locus_tag="ACHK0Z_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU4502010.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26195..26917
                     /gene="baeR"
                     /locus_tag="ACHK0Z_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             26195..26917
                     /gene="baeR"
                     /locus_tag="ACHK0Z_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU4502011.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27108..27440
                     /gene="yegP"
                     /locus_tag="ACHK0Z_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             27108..27440
                     /gene="yegP"
                     /locus_tag="ACHK0Z_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU4502012.1"
                     /translation="MAGWFELSESSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27649..27945
                     /locus_tag="ACHK0Z_RS09920"
                     /old_locus_tag="pgaptmp_001984"
     CDS             27649..27945
                     /locus_tag="ACHK0Z_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4502013.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27947..28243
                     /locus_tag="ACHK0Z_RS09925"
                     /old_locus_tag="pgaptmp_001985"
     CDS             27947..28243
                     /locus_tag="ACHK0Z_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4502014.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28346..29707
                     /gene="yegQ"
                     /locus_tag="ACHK0Z_RS09930"
                     /old_locus_tag="pgaptmp_001986"
     CDS             28346..29707
                     /gene="yegQ"
                     /locus_tag="ACHK0Z_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU4502015.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30037..>30276)
                     /gene="yegR"
                     /locus_tag="ACHK0Z_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             complement(30037..>30276)
                     /gene="yegR"
                     /locus_tag="ACHK0Z_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU4502016.1"
                     /translation="IGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIVKNDA
                     AHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6983 a         8076 c         8015 g         7202 t
ORIGIN      
        1 taatcctcaa ataatgtgag ataaatagtg ccgcaacatt gcatttttgc cctgatttat
       61 ccatgatcga attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg
      121 gattgccgat ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct
      181 cggcattgat aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaagcagcg
      241 cgaccgcgca cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctggc
      301 gtcaatctcc tggctggtca ccctgacgca accgctgttc agcttccgct cgtttacctt
      361 tagcgcccgc gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat
      421 ggagctgaac gaacggctgg aaggaaaaga cagcaataac cccacacaac gcaaaggtgc
      481 gaagttctgg ggggtggtga cacaaattgt ggtactggac gccatcttct cactcgactc
      541 ggtgattacc gccgtcggga tggtcgacca tttactggtc atgatggccg ccgttgttat
      601 cgctatcagc ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc
      661 gaccatcgtt attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga
      721 aggtttcggc ttcgtcattc cgaaaggcta cctgtacgcc gccattggct tctcggtgat
      781 gatcgaggcg ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaacca
      841 gacgctacgc cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga
      901 tgcggaactg gacgccgaaa ccgcgtccat gctggtggat catggtaacc agcagatctt
      961 taatccgcag gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtactgtcag
     1021 cagcattatg acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagaaga
     1081 aatccgccag ttgctggagc gaaatcagca tacgcggctg gtggttactg atggtgatga
     1141 cgcagaagat ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt ctctgcgcgg
     1201 cgaaccgctc aacctgcggg tgttgattcg ccagccactg gtgttcccgg aaaccttgcc
     1261 gttgttacct gccctcgagc agttccgtaa tgcccgcaca cactttgctt ttgtggtgga
     1321 tgagtttggc tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgccgg
     1381 taacttaccg aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga
     1441 cggttcctgg acggcgaatg gtcatatgcc gctggaagat ctggtgcaat atgtgccgct
     1501 gccgctggat gaaaaacgtg aatatcacac cattgccggg ctgttgatgg aatatttaca
     1561 gcgtattcca aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca
     1621 ggtggaaagc catcgcgtgc agaaggtgca aattataccg ctacgtaagg atggcgagat
     1681 ggagtacgag gtgtgatggt atcggccatt ttacccaatg ctgcgtaacg ccttataact
     1741 tcagcaatga agcgttgtct gtggtaaacg gacatcacag cgggggtgtg ggtccggctg
     1801 taaatcgccg aatcgtttcc tgtaggccgg ggcagcccac atggatgtgg gctgagggcg
     1861 cgtcttacag gacgttacct cgcgcccgta cccgatagcc gaaaggaata agatgaggga
     1921 accgcgccag cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc
     1981 caccctggca cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg
     2041 aacagccttg aatcttacat attcagaata taatggtgcc gccgcgaacg cctgatgcga
     2101 cgctttacgc gtcttatcag gcctacagga caggtcacct ggtaatctac atcttctcca
     2161 gcaacttctt cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca
     2221 ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagcag ctggtccact tgcaggctgt
     2281 aattgagttg ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat
     2341 caatcagttt gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc
     2401 aggtttgatc tgccagattc aacatacctt cccctgtcag cgccagcaca ggcgattgac
     2461 cttgcatgtc gtttaacgtc acgacgccat ccttcaatgt caaatcggtg gtaaagcggt
     2521 caagccgcgt cacgttatcg aagttttcag cggccttcac atcaccacca ttacgttcta
     2581 ccgcttgctg aatcatctgc tggaagttca tcccttccat gcgcgtgtcg gtcatttcga
     2641 catgtgcctg tccttgccag ttatggcgga atgcgtcggc atctatgtca gcaccggaga
     2701 aatcaccagc cagtgacatc tttccggtca acgaaatcgg atagttaaac gccttcagaa
     2761 tggtaccaat ctcaacgttt tccagccgtg gctggaagtt tatccgcgga tttattgatg
     2821 tcgcgtccag cgtgcccggc agtgaaacct ttccaccgtt aagcttgccc tgcagttgag
     2881 taatttccag caaacccgac ttgttggtca tttgcgtggc aacatatgta aaattcattc
     2941 cgcgccagcg cacgttactg gcttgcaaca aaatatcagc cgtaaagcct tgcagtccct
     3001 gataggccgg ttcatcaata cgcgaagaaa taaccgggcg cggcaacgtt gattggctct
     3061 gcccctgctg cgcggcaccg ttttcaccat tcgcggtttc attaagcggg atgaggttgt
     3121 caagattcag ttgcgggaat tgcagtctca gctgccattc aggtttctct gtcagcgtga
     3181 cctgtgcttg cccgctcagc gtgctgtcat tggcagtcaa gctgatttgg ttaaatgaaa
     3241 ggcgtttatg tgactcctgc cactgggcct ggaaactccc ctgcccctga ataccttgtt
     3301 ttggtaaatc tgcaccctgc aactgccagt taatttgttc aatagccgcc gttaaatcat
     3361 gcggataatc agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc
     3421 gattaacccg cccggagaac tcaaatgagc cacgatgttg gggatcttgt tccatttgca
     3481 ggcggatatt gcggattgtc acctgctcgt catcttcatg ctggaacacc agcacgctgt
     3541 ccgccacctt aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca
     3601 aggtattgtc gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg
     3661 tcagttggat cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta
     3721 gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc
     3781 cctgggcggt gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac
     3841 gcagtggccc gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat
     3901 agtcgcggaa atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca
     3961 cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga
     4021 attagtcttt atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt
     4081 cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa
     4141 ttaacatgcc cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa
     4201 tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac
     4261 gcgccaaaga ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact
     4321 ccagcgtcac cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga
     4381 gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc
     4441 tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta
     4501 catccaccgt cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc
     4561 cttcatcaag ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg
     4621 cgcatcactc ccttaacgcc aattacgtca agggcatacc aggttatcac tgaacggtac
     4681 acaatttatc aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg
     4741 atgcggtttt tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg
     4801 aattgcagga acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa
     4861 cgcccacgct cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta
     4921 tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg
     4981 ccttcgagaa tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt
     5041 tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc
     5101 tcaagcaaca ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct
     5161 tccatcgaca gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt
     5221 tcatcaccga cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg
     5281 gaagccgatg cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat
     5341 ttagcgacct gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat
     5401 tatagggatt tcatccgcct gataccagtc gaatagcgct gccgcgcgct cagagttaat
     5461 tgttgacgaa gaattcccgg tggcaaatta cgttgatcag ttttatacaa ggtaaaaaat
     5521 gttatacgca gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata
     5581 aaatgtaaaa cttttgtact agcataaaca cagaaacgaa tactggcgac ctggtcttgc
     5641 ggataaagcg gtaatgagca aacaatcaca gcatgtatta attgccctgc cccacccgct
     5701 gcttcacctg gtcagtttag gtttagtctc gtttatcttt acccttttct cgcttgagct
     5761 ttcgcagttt ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc
     5821 gttttatcgc catgccgggc gcatgtggcc gggaattgcg ctgagctgct cgctgggaaa
     5881 tatcgccgca tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat
     5941 caatattgtt gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa
     6001 ccccttgcaa aatctggctg actggctgcg tctggcattc ggcagcgcca ttgttccgcc
     6061 tctgctaggg ggtgttctgg ttgtcctgct gacgcccgga gacgatcctc tcagggcatt
     6121 tttgatatgg gtactgtcag aatccatcgg cgctctggca ctggtgccgc tgggattgtt
     6181 atttaaacca cactatctgc tgcgccatcg caacccacgg ttgctttttg agtcgctgct
     6241 cacgttagcc atcacactga cgttaagctg gctttcgatg ctgtatctgc cgtggccttt
     6301 tactttcatt attgtgctgt tgatgtggag cgccgtgcgc ctgccacgaa tggaagcctt
     6361 tttgatcttc cttaccacgg tgatgatggt gtcgctgatg atggccgcgg atccctccct
     6421 gcttgctacg ccgcgtacat acctgatgag ccatatgccg tggctaccgt ttttgctgat
     6481 cctgctgccc gccaacatca tgacgatggt gatgtatgcc tttcgtgcgg aacgcaaaca
     6541 catttccgaa agcgaaaccc gttttcggaa cgccatggaa tattccgcta tcggtatggc
     6601 gttagtgggc accgagggac aatggctgca aaccaacaaa gcgctctgcc agtttctcgg
     6661 gtacagtcag gaagagctgc gcggactcac ctttcagcaa ctgacctggc cggaggatct
     6721 caataaagat ctccaacagg ttgaaaagct gataagcggt gaaataaaca cctattcaat
     6781 ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg gcgttgcttg ccgtctcact
     6841 ggtgcgccac acggatggca cgccgctcta ttttatcgct cagattgaag acattaacga
     6901 gctaaaacgc accgaacagg tgaatcagca actgatggag cgcatcacgc tggctaacga
     6961 agcgggcggg attggcatct gggagtggga gttgaagccg aatattttta gctgggataa
     7021 gcggatgttc gagctgtatg aaattcctcc acatatcaaa ccgaactggc aggtgtggta
     7081 cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa gtgattcgtg attcgttgca
     7141 atcacgctcg ccctttaaac tggaatttcg cattaccgta aaagacggta ttcgccatat
     7201 ccgcgccctc gccaaccggg tactgaataa agaaggcgaa gtcgaacgcc tgctcggcat
     7261 taatatggat atgactgagg ttaaacagct taacgaggca ttgtttcagg aaaaagagcg
     7321 cctgcacata acgctggact ccatcggcga agccgtggtc tgtattgata tggcaatgaa
     7381 aattaccttt atgaatccag tggcggagaa gatgagcggc tggacgcagg aagaagcatt
     7441 aggtgttccg ctcctgacgg tgttgcatat tacctttggc gacaatggtc ccctgatgga
     7501 gaacatttac agtgccgaca cctcacgttc cgcgattgaa caagatgtgg tgttgcactg
     7561 ccggagcggc ggcagctacg acgtgcatta cagtattacg ccgttaagta ctctggacgg
     7621 cagcaatatt ggttcggttc tggtgattca ggacgttacc gaatcacgca aaatgctgcg
     7681 ccagctgagc tacagcgcct cccatgatgc actgacgcat ctcgccaatc gcgccagttt
     7741 tgagaagcaa ctgcgtatgc tgctgcaaac ggtaaacagt acacatcagc gacatgccct
     7801 ggtgtttatc gatcttgatc gctttaaagc ggtgaatgac agcgccgggc atgcggcggg
     7861 cgacgctttg ctgcgcgaac tggcgtcgtt gatgctgagt atgctgcgtt ccagcgacgt
     7921 gctggcgcga ctcggtgggg atgaatttgg tctgctgcta ccagactgta atgttgaaag
     7981 cgcgcgtttt atcgctacac gcattatcag tgccgtgaat gactatcact ttatatggga
     8041 aggccgtgtg catcgggtag gtgccagtgc cgggattacc ttgattgatg acaacaatca
     8101 tcaggcggca gaagtgatgt cgcaggctga tatcgcctgt tatgcctcca aaaatggtgg
     8161 acggggccgg gtgacggttt acgaaccaca gcaagctgcc gcacatagcg aacgggcggc
     8221 gatgtcgctt gatgaacagt ggcggatgat taaagagaat cagttgatga tgatggccca
     8281 cggtgtcgct tcgccgcgga tcccggaagc gcgtaatttg tggctgattt cgcttaaact
     8341 ctggagttgc gaaggcgaga ttattgatga acaaaaattt cgtcgtagct tcagcgatcc
     8401 ggcacttagc catgctcttg accgacgggt attccacgaa tttttccagc tggccgcaaa
     8461 agcggttgcc agtaaaggca taagcatcgc cctccccctt tccgttgccg gtttgagtag
     8521 cgccacgctg gtgaatgatc tgcttgagca gctggaaaat agccctctac caccacggtt
     8581 attacatctg attattccgg cagacgcgat tttagatcac gcagaaagcg tgcaaaaact
     8641 gcggctggcg ggatgtcgga tagtgctcag ccaggtgggc cgcgatctgc aaatcttcaa
     8701 ctcgctgaaa gcgaatatgg cagattacct gctacttgat ggtgagttat gcgccaacgt
     8761 gcagggtaat ttgatggatg agatgctgat tacgattatt caggggcacg ctcagcgact
     8821 cgggatgaaa accatcgccg ggccagtcgt tttaccctta gtgatggata cgctttctgg
     8881 catcggcgtc gatctgattt atggtgatgt gattgccgat gcccaaccgc tggatttgct
     8941 ggtgaatagc agttatttcg cgattaactg agggagcgtt tgcccattgc ctgatgcgac
     9001 gcatcaggcc tacaattcgt ccgttccgtc tggttgccag ccttccgtat accagatatg
     9061 caacagcgca taagaacgcc agggcttcca gcgctcggca taacggcgga tttgtgccgg
     9121 tgtcatcccc ggaaatcgct gtttaatcag ataatcatcc ggcagaaaaa catctttcgc
     9181 ctgccagcca cgcaaagcga aataattcgc cgtccagcgc ccgatacccg gaaaagtttg
     9241 cagcgttttc atcgcctgct ccacatcgcc cggaattgtc attggtaagc tgccctccag
     9301 cgccgcattt gccagatgaa tcagtgcctc tgcccgtttc aacggcatgc ctaacgcttt
     9361 taatgcctgc gggtcggctg ctgccagccg ctgaggcgtg gggaagcaga tatactccgg
     9421 aaaatcatcc agccgttcgc cataaagctg tacaactttg gcagtcaatt ttgccgccat
     9481 cgccacgctc accagttggc ctaaaatcgc ccgcacaccc tgctcaaaag catcaacaca
     9541 gccgggtaaa cgcaatccgg gccgcgccgc gcctaacttg cccaacgcac cgttaacaat
     9601 ctgcgggtta cattgcagat caaacaggcg gctcattttc gccagacact cagcggcaac
     9661 gggttctaaa cctgcactta aatttatgtg cagagtatgg cgggctatat ccggaatagc
     9721 agtcaccacg ccgcgatatt cgcccaccgc cagactacgg gcataataat cctcagcgac
     9781 cgtttccaca ccactcactg cacgggcggc gagaaatccc aacatccacg accagtcata
     9841 cggcggctgc cagttcaggg tatacatcgc atctccttat tcatccgctt tcagcataaa
     9901 cgttattcag acgctacgct ttgctttcat attccggttg tcgcgacagc aacatttcgc
     9961 taaagtcacg ccccttcttc accggcatgg ggattaattc gtgtttattg gttttgatta
    10021 cggtacagca aactgttcag tggcggtcat gcgtgacggt aaaccgcaat tactgaaaat
    10081 ggaaaacgac agcacgctgc tgccttcaat gctttgcgcg ccaacgcgtg aatcggtaag
    10141 cgaatggctg taccgccatc atgatgttcc ggcagacgac gatgaaacgc aggcgctgtt
    10201 acgtcgggcg attcgttata accgcgaaga agatatcgat gttacggcga aaagcgtgca
    10261 gttcggtctt tcctcactgg cacagtacat tgatgatcca gaagaagtgt ggtttgtgaa
    10321 atcaccaaaa tcgttcctcg gtgccagcgg cttaaaaccg cagcaggtag cgctgtttga
    10381 ggatctggtc tgcgcaatga tgttgcacat tcgccagcag gcgcaggcgc agctgccaga
    10441 agcgattact caggcggtga ttggtcgccc gatcaacttc caggggctgg gcggcgatga
    10501 agcaaacgcc caggcgcaag ggattctgga acgcgcggcg aagcgtgccg gattcaggga
    10561 cgtggtattc cagtacgagc cggtcgcggc tgggctggat tacgaagcca ccttgcagga
    10621 agaaaaacgg gtgctggtgg tggatatcgg cggtggtacg actgactgtt cattgctgct
    10681 gatggggccg cagtggcgtt cgcgtctcga tcgtgaagcc agcctgctgg gtcacagtgg
    10741 ttgccgtatt ggcggtaacg atctggacat cgcactggcg tttaaaaacc tgatgccgtt
    10801 actgggcatg ggtggcgaaa ccgaaaaagg catcgccctg ccgattctgc cgtggtggaa
    10861 tgcggttgcc atcaacgacg tacctgcgca gagtgatttc tacagtagtg ccaacggtcg
    10921 tctgcttaac gatctggtac gcgatgcccg cgaaccggaa aaagtggccc tgctacagaa
    10981 agtctggcgt cagcgtttaa gctatcgcct ggtgcgtagc gcagaagagt gcaaaattgc
    11041 tctttcaagc gtagcggaaa cccgcgcctc actgccgttt atcagcgatg aactggctac
    11101 gctgattagc cagcaagggc tggaaagcgc cctcagccag ccactggcgc ggattctgga
    11161 acaggtgcaa ctggccctgg ataacgccca ggaaaaaccg gacgttatct acctgaccgg
    11221 cggtagcgcc cgttcgccgc tgattaaaaa agcgctggca gaacagttgc cgggaattcc
    11281 gattgcaggc ggcgatgact ttggctccgt caccgccggg ctggcacgct gggcggaagt
    11341 ggtgtttcgt taaaagaaaa tggctcagca gcggcttaaa tctgcctgcg cctgagccag
    11401 tttttctgcc gcctgacgta aaggttccat caacggcatg gtccgtgctg gagcatgaag
    11461 gcggaatcgc tgcaactctg tcgcgccgac agtcaacgtc gattccagcc gatgtcgttt
    11521 ggcggtcatt ttcgccatta ccgcttgtct gtctgctggc gttatcgctt cattcggcct
    11581 gaacacaaaa cggcgttcac agctcgcttt ccagtcgata accgcctggg tcagatggtc
    11641 gccaaaccct ttcacttgct taacgccacg acgggtaaca tctgcggctg tttcaatacc
    11701 aaaagaccgt aacgccgctt tacgcgcagg gccaacgccg ggaatagagg caacatcaat
    11761 aaaaaatccc tccagaaact tctgcttctg ccgttccctt gcagtgtcat gaagtgctgc
    11821 cagagcgcgt ttctcttctt ccggtaaccc gagaatttcg tccttcattt tttcgagcat
    11881 cgcccgtttg gcgataaaac cttccagccc gcccaactgt tggatctggc tgactaaatg
    11941 ctcatagtcc attttcgcgc gattaaatgc ctcacggcgt tgctggattt ctgctttgta
    12001 cgctttgctt gtcagactgc caataatcca gatagccgac agcacaggaa ccaaaataat
    12061 ataacgcggt tctgcctgaa ggccgcaaag caacagcgat aacgctgaca gtgcgatctc
    12121 aatcagaatg atgtattcgc gccgtaacag gcctaaagga agcggcctgc cagtcggttg
    12181 gaaatgatcg ggtaatggca attgcaatgc tggcggtgct actgacgcca tcaccatcgc
    12241 ccagactttc gccagcacaa aatcaccgcc ggtggtaatg acctcttcgc cgagatcaat
    12301 aaaatagata acgccttgat tatccagcgc acaccacggg cagtcggtca aatgagcggg
    12361 ataaacatgc attgccgaaa cgggacattt ctttaactgt tggcgcagag aatcaagtgc
    12421 cgctacccac gctttagccg tcggacgccc ggttgccacg ccactttccg tgaatgcctg
    12481 ctgaaacatg gcttcaacat cgcccggtaa catcgatagc ggaatcgatc gtggcggcgg
    12541 ttttaaacca cgtcgctgat tatctgacgc gtaggcataa cggaaatggg taatatccgt
    12601 ctccagcgca ttacccgcat cagagataag cggcacacca gaataaggat gccggccacc
    12661 aaacaagacg tgaaaaatca gcaacgcaag gccaaaatta tcgtgatttt ctgtgcgttc
    12721 aaagccgaca aatgacgaca aggtttgtag ctctggcggc gtaaaatgcg acacgccgac
    12781 ttcgcataaa tgcagtgtgc cattggcgtt aatctgaaag gagtcgctat caatcagtac
    12841 cactttgctg tcgcgaccta ccataaaact gttctggttt acgtcaccca cgacgtgccc
    12901 gtgctcgtga accgtagcaa aagatgaagc aatattgcgc gcaacataga gtagaaaatc
    12961 ccacgcacaa tgagggtaac gttgacgacg atgtgccggg ctatagatca tatgaatcgg
    13021 ttctttacca gaaacttttg gcatcataaa accgataact tttccgcctc gtccaccgtg
    13081 aagcgttgcc tgcggccagg cgacataatt caacaattgc gcgtcagctg tcgcagccat
    13141 aaaggcaagt ttgtcctgtt ttaaggcggg tggcggcgtg tgataaatct tggcgacgct
    13201 atcgacaaac tcattgatat cataaaccgc gccttcaccg cctttgccca gttcacggcc
    13261 tggcgtaacg cactcaccag tagcagtata taaagtgggt ttcatggtaa ccacagcgcc
    13321 agtgccaacg ttttatcatc atccgtacgc tcgtttaccg ccgggctgga caaaaactgc
    13381 tttaacaatt caggtaataa atcgagttgt tcctgcgttg ctgacgccag tccattgaaa
    13441 aacggggtaa agaaaggtac atgaggagaa ttatccagca tatttaacgc cagtcgctgg
    13501 ataccatccg taaatgctgc aactttatgc acacgctcag tgctggtgaa agtttccagt
    13561 ctggaaacgg catcttcatc ggtaatgaag tgcgtcatat tggcatattc acccaccatc
    13621 ggcgtaagcg gcaattgcag accgtgacca aaatccacca ccacgccgcc atcgccaatc
    13681 tgcataatca aggtgccatt agccgatgaa atcaatccca gaaacgtgca ggcaaaatcg
    13741 cgaactgcta attctttggc ttccgcctcg gcaaaaagtc gctgacgaac ggtcagcaca
    13801 atatccgtgg cgaggatatc attaagcccc aattccccac cctgcacttt ttgcgacata
    13861 taagccatcg cttcattgac ggcgagcgtc gcgccttctc ctccctgcga aacgctacct
    13921 gcgccatcag caaggaacat caccagtaat ggctgctggt catttagcca ggcaacctgc
    13981 atctggcagg catcctggca aggcaagtcc gcgctgatgt gcgatgttcc aacagcggat
    14041 gcataaacca gacgccagct cacactgacg tccagccttt tggcgcttcc agcacaactt
    14101 ccgttcccgg cgtggagcgg gaaaccgaac gcagcgaact ggataaccag ctaaacagtt
    14161 cacggaattg taatccttgc agaggcaaag gctgacgaac gctgatttgt gccagagtct
    14221 tcatatccgc cccctgaaca ccgatggaaa agaaagcaaa ccttttatct tcttcccccc
    14281 gaaacacttt gttggctgca gcctgccact catcggttgg tgcaccatcg gtaatcagga
    14341 aaatccacgg gcgataatac gaaataccat ttgcacgata ctcacgtttc cgctcctcta
    14401 ccatatccag ggctttggta atggccgcac ccattggtgt atcgccctgg gcaaacagga
    14461 tgggcggaaa aaaattagcg gcgctggtaa agggttgttc cacatgcacc gggccgaacg
    14521 tcacaatgcc gagttccact ctttttaaag ccagcggatc ggcaagcagt tcatcacgaa
    14581 aggtcaccaa tccggcgtta agttcattaa taggtctgcc gctcatcgag ccagaaacat
    14641 ccagcagcaa aatgcagggg caacgtggtt ccgggttact ggcaaaatcg ctggtggcaa
    14701 acgtgatttg ttcgctcata agaaatcctt tcttagagaa ttatcgaggt ccggtatcat
    14761 accgactgca ataatgaaat aagagcaaac gaagaaactt tcagaattag gcccggcggc
    14821 gcagaaaatt ttgctattcg cctcgcaaga acggagagcc gtcgattgac actaagggcg
    14881 gagtgacata atttcaggag tgagggttag ggagaggttt ccccctcccc ctggtgttct
    14941 tagtaagcct ggaagctaat cactaagagt atcaccagta tgatgacgtg cttcatcata
    15001 accctttcct tattaaaagc cctcttctcc gggagaggct ttcccgtttc agcgtcccgc
    15061 tgaaatcatc ggcttacctc ctttcgccct gaatgcagtc tatcgctaac gcgtgaataa
    15121 aatagtttcc tctgtgatta ttgcgtaacg actcgcaaag aatatttgct ggcaggatcg
    15181 cagactacaa agcctgcgga ttgacaatct tatcgtgaag gcatactttc aggagtgagg
    15241 gtagagcggg gtttcccccg ccctggtagt cttagtaagc ggggaagctt atgactaaga
    15301 gcaccacgat gatgagtagc ttcatcatga ccctttcctt atttaaggcc ccttcctcgg
    15361 gaggggcttt cccgtttcag cgtcccgctg aaatcgtcgg cttacctcct ttcgccatgc
    15421 acttagtcta tcgctgactt gcagataaaa tagtttccag tgtgattatt gcgtaacgtc
    15481 tcgcaaagaa tttttgctgg caggatcgca gacggcaaag cctgcggatt gacaatctta
    15541 tcgtgaaggc atactttcag gagtgagggt agagcggggt ttcccccgcc ctggtagtct
    15601 tagtatgctg ggtagctaat cactaagagc accacgatga tgagtagctt catcatgacc
    15661 ctttccttat ttaaggcccc ttcctcggga ggggctttcc cgtttcagca tccggctgaa
    15721 atctgcggct tacctccttt cgccctgtat gcagtctatc gctaacgcgt agataaaata
    15781 gtttcctgtg ttattactgg atgcgtgctc gcaaatgtgc ccgccattca gacgattccc
    15841 gatagtgttt cataattcct ccatttttct cccttattgg ctggctacac tagtatcatt
    15901 ccgcgaaacg tttcaggaag agaaactctt aacgatgaaa ggcagttata aatcccgttg
    15961 ggtaatcgta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    16021 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    16081 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    16141 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt accgccgcta atactgttac
    16201 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    16261 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    16321 agcccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    16381 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    16441 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    16501 gcagctgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    16561 gcaggttgat gttggtaacc aaatctccag tggtgatacc accgggatcg tggtgatcac
    16621 ccagacgcat cctatcgatt tgctctttac cctgccggaa agcgatatcg ctaccgtcgt
    16681 gcaggcgcag aaagccggaa aaccgctggt ggtagaagcc tgggatcgca ccaactcgaa
    16741 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    16801 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    16861 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cggccgccct
    16921 gcaaatgggc aatgaaggcc attttgtctg ggtgctgagt agcgaaaaca aggtcagcaa
    16981 acatctagtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    17041 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    17101 agtggtggaa gcccagagcg ccactactcc ggaagagaaa gccaccagcc gcgaatacgc
    17161 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    17221 cgcctgttta ttatgcgtcc tgtggccacc acgctgctga tggtggcgat cttactcgcc
    17281 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    17341 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    17401 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    17461 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    17521 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    17581 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    17641 acctcaaccg ccatgccgat gacccaggta gaagatatgg tggaaacccg cgtcgcgcag
    17701 aaaatctcgc agatttccgg tgtcggactg gtgacgcttt ccggcggtca gcgtccggct
    17761 gttcgcgtca aacttaacgc ccaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    17821 cgcaccgcca ttaccggagc taacgtcaac tcggcaaaag gtagcctcga cggcccttcc
    17881 cgtgcggtca cgctttccgc taacgaccag atgcaatccg ccgaagagta tcgccagcta
    17941 atcatcgcct accagaacgg cgcaccgatt cgtctgggcg atgtcgcaac cgtagagcaa
    18001 ggagcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    18061 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcatccg gcagatgctg
    18121 ccacagctca ccgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    18181 accaatattc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    18241 gtagtcatga tcatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    18301 gctgtaccgc tgtcgttaat cggcactttt gcggttatgg tgtttctcga tttttcaatc
    18361 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    18421 gtagtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    18481 ctcaaaggcg cgggtgaaat cggctttacc attatctcgc tgaccttctc gctgattgcg
    18541 gtgttgattc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    18601 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    18661 atgtgcgcgc ggatgctcag ccaggagtcg ctgcgtaaac agaaccgatt ctcccgtgcc
    18721 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaggtgctg
    18781 aatcatccgt ggctgacctt aagcgtggcg ctcagcacgc tgctgcttag cgttctctta
    18841 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca acggcattat tcagggcact
    18901 ttgcaggcac cgcaatccag ctcctttgcc aatatggccc agcgacaacg ccaggtcgcg
    18961 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    19021 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgct ggatgaacgt
    19081 gatgaccgag tgcaaaaagt cattgcccgt ctgcaaacgg cggtggataa agtgccgggc
    19141 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    19201 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    19261 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    19321 aaagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    19381 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    19441 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    19501 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    19561 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    19621 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    19681 gcgattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcagttccag
    19741 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    19801 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    19861 attctctcga cgctacccac cgcaggggtt ggcgcactgc tggcgttgct gattgctggt
    19921 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat cgtgaagaag
    19981 aacgccatca tgatgatcga cttcgcgctg gctgccgagc gcgagcaagg catgtcgccg
    20041 cgcgaggcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccactctg
    20101 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    20161 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttt
    20221 accacgccgg tgatttattt gctgttcgac cgcctggcat tgtggaccaa aagctgcttt
    20281 gcccgtcatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    20341 gcgacgattt tactgtcggt tgccattacc ctgtgcggca tactgggctt ccgtatgctg
    20401 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    20461 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcacttggg
    20521 cgcattgccg gagtcagtga aatgacctcc agcagttcgc tcggcagcac gcgtattatt
    20581 ttgcagtttg attttgaccg ggatatcaac ggcgcggcgc gcgatgtgca ggcggcgatc
    20641 aacgctgcac aaagtttgct gcccagcggg atgcccagcc gtccgaccta tcgcaaagct
    20701 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    20761 gaactgtacg attttgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    20821 ggtgatgtcg atgtcggcgg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    20881 gcgctgttta atcagggcgt ctcgctggac gacgtacgca ctgccatcag caatgccaac
    20941 gtgcgtaaac cgcagggggc gctggaagat ggcactcacc gctggcagat ccagaccaat
    21001 gatgagctaa aaaccgccga cgaatatcag ccgctgatta ttcattacaa caacggcggc
    21061 gcggttcgtc tgggcgatgt ggcgacagtg accgactctg tacaggatgt acgtaacgcc
    21121 gggatgacca atgccaaacc ggctattttg ctgatgatcc gcaagctgcc ggaagccaat
    21181 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    21241 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgctc ctcgctggaa
    21301 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt atttttattc
    21361 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    21421 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    21481 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt
    21541 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    21601 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    21661 ggcggattgc cgggccgact gttacgcgaa tttgccgtga cgctttctgt cgccattggt
    21721 atatcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    21781 agcaagccgc gcgagcaaaa gcgaatgcgt ggttttggtc gcatgttggt agccctgcaa
    21841 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    21901 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    21961 ccggagcagg acacaggcgt gttgatgggc ggtattcagg cggatcagag tatttcgttt
    22021 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    22081 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    22141 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    22201 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    22261 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    22321 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    22381 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgtgacacc
    22441 atggcacggc tgggaatcga cgtacaagcc gccaacagtc tgttaaataa cgccttcggt
    22501 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    22561 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    22621 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc cccactatcg
    22681 gtgaatcatc agggattatc ggcggcctcg accatttcgt ttaacctgcc gaccggaaaa
    22741 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    22801 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    22861 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    22921 agttatgtgc acccgctgac gattctctcc accctgccct cggcgggtgt tggagcgctg
    22981 ctggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta
    23041 ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa
    23101 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    23161 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    23221 ggtgacggct cggagctgcg gcaacccctg gggatcacca ttgtcggcgg tctggtaatg
    23281 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    23341 ctgcgttttt cgcgtaaacc caaacaaacg gtaaccgagt aaatgacaga tcttcccgac
    23401 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    23461 accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    23521 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    23581 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    23641 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    23701 ttacagggcg ttggcggcgc gatgatggtg ccggtcggca gattgacggt gatgaaaatc
    23761 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    23821 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    23881 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat gttaatgccg
    23941 aactatacca tgcagacgcg gcgcttcgat ctctccggct ttttattgct ggcggttggc
    24001 atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctgacg
    24061 attgctggcc tggtcgcagt tggcgtggta gcactggtgc tttatttgct gcacgccaga
    24121 aataacaacc gcgctctttt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    24181 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    24241 ttcctgcaaa ttggcctcgg tttctcaccg tttcatgccg gactgatgat gatcccgatg
    24301 gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg ctttggttat
    24361 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact
    24421 accgccctgc tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    24481 aactcgacgc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    24541 gcgagcagcg gcaacagcct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    24601 actatcgccg ggctgttgct gggacttttt ggttcacagc atgtcagcgt cgacagcggc
    24661 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgttgatcat cgcccttccg
    24721 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    24781 aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga
    24841 ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    24901 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    24961 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    25021 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    25081 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    25141 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    25201 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    25261 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgct ctggcaacgt
    25321 tactcgcggc gctcgccacc tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    25381 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    25441 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    25501 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    25561 tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgtaaa ttcacgccgg
    25621 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    25681 atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga
    25741 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagt cgtggcctga
    25801 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    25861 agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc ggcagcctgc
    25921 aaatctctgc cgggcagcgc gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    25981 gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc gaaggttccc
    26041 gcaaccgtgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    26101 cacataatgg tcgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    26161 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    26221 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    26281 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta
    26341 tgtgcgccag acaccaccgg atctgatcct gttagatctg atgctccctg gcaccgatgg
    26401 cctgacgctg tgccgggaaa ttcgtcgttt ttctgacatt ccgatcgtga tggtgacggc
    26461 aaaaatcgaa gagatcgatc gcctgctggg gctggagatt ggcgcagatg attatatctg
    26521 taagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    26581 accgcagcgc gagttgcagc aacaggatgc tgaaagcccg ttgattatcg acgaaggtcg
    26641 ttttcaggct tcatggcgcg gtaaaatgct tgacctgaca cctgcggaat ttcgtctgtt
    26701 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    26761 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg
    26821 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgtcgg
    26881 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt attttgttag
    26941 ccggagatgg cgttcagacc cgatccggca acattatccc acgcatggtc agcaaactga
    27001 cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc ggtaaacagc
    27061 acctttagat gctgttttcg atacacaatt tcaatcaagg agtcattatg gctggttggt
    27121 ttgaactcag tgagagcagt gataatcagt tccggtttgt gctaaaagcg ggcaatggtg
    27181 agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt
    27241 cggtgcgtag caacagcccg caagaagaac gctatgagaa aaaaacggca agtaacggca
    27301 aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc cagatgtacg
    27361 ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga
    27421 cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc cggctttata
    27481 tccaaataac gcttagttat taataattaa gagtaaccgc cgccattaaa atagtgggca
    27541 ctgacgagca ctcgttgtgc tttctttgtt taccatccca tacttatcct cctgaacatg
    27601 ttcatgtgcc aaaatatctt ctaatacttt cgtgacgcta tccggtttat gacatacact
    27661 gatgtaagat ttttacttac aggcgggcgt aaaatggcca gaacgatgac ggttgatctc
    27721 ggcgatgagt tgcgtgaatt tatcgagtcc ttaattgaat ccggcgacta tcgcacccaa
    27781 agcgaagtga tccgtgagtc actgcgatta cttcgagaaa agcaagcaga gtctcgcctg
    27841 caagcgttac gtgacatgct cgcagaaggg ttgagtagtg gtgaagctca gccgtgggaa
    27901 aaggacgcgt tcttacggaa ggtgaaagca gggataagga aataatgtgc gtattattaa
    27961 actcatgcca aaagccaatg aggatttaga aggcatctgg tattacagct atcaccattt
    28021 cggcgaacca caagccgaca gatatgtgga acatctttca gacgttctcc agattctgag
    28081 taacaataat ataggtacgc ccagacctga attaggcgaa ggtatttttg ttcttccttt
    28141 tgaacgccac gtcatttatt tcctgcaatc accaggggaa attatcgtca ttcgtatcct
    28201 taaccaaaac caggacgcca ccagacattt acactggagc taaacccgcc gattgcgcta
    28261 caatgcccgc ccttaaagtg ggggcactcc cctaaccgct tcatcaggtg aagcggatct
    28321 gacctgtcat cagaacgaga gaattatgtt taaaccggaa ctcctttccc cggcgggaac
    28381 gctgaaaaat atgcgttacg ctttcgctta tggcgcagat gctgtttatg cgggccagcc
    28441 gcgttattcc ctgcgtgtgc gcaacaacga attcaaccac gaaaatcttc agctcggcat
    28501 caatgaagcc cacgcgctgg ggaaaaagtt ttatgtcgtg gtcaacattg caccgcacaa
    28561 cgccaagctg aaaaccttta tccgtgacct gaaaccggtg gtggaaatgg ggccggatgc
    28621 gctgattatg tccgatccag ggctgattat gctggtgcgt gagcacttcc ctgaaatgcc
    28681 gatccacctt tcggtgcagg ctaacgccgt gaactgggcg acggtgaaat tctggcagca
    28741 aatgggcctg acccgcgtga tcctctctcg cgagctgtcg ctggaagaga ttgaagagat
    28801 ccgcaatcag gtgccggata tggagatcga gatcttcgtt cacggcgcgc tgtgcatggc
    28861 ctactccggt cgctgcctgc tctctggcta tatcaacaag cgcgacccga accagggcac
    28921 ctgcaccaac gcctgccgct gggagtacaa cgtccaggaa gggaaagaag atgatgttgg
    28981 caacatcgta cacaagtacg agccgattcc ggtgcaaaat gttgagccga cgctgggtat
    29041 cggcgcacca accgacaaag tgtttatgat cgaagaggcc cagcgtccgg gcgagtatat
    29101 gaccgcgttt gaagatgagc acggcactta catcatgaac tcgaaagatc tgcgcgccat
    29161 cgcccatgta gaacgcctga ccaaaatggg cgtgcattcg ctgaaaatcg aaggtcgtac
    29221 caaatctttc tactattgtg cacgcaccgc acaggtttac cgcaaagcta tcgatgacgc
    29281 cgctgcggga aaaccgttcg ataccagcct gctggaaact ctggaaggtc tggcgcatcg
    29341 tggctatacc gaaggtttcc tgcgtcgtca tactcacgac gattatcaga actacgaata
    29401 cggttattca gtttctgacc gccagcagtt tgttggtgag tttaccggtg agcgcaaggg
    29461 ggacctcgcg gcggtagcgg tgaaaaataa attctccgtt ggcgacagcc ttgagctgat
    29521 gacgccgcaa ggcaacatta attttaccct tgagcacatg gaaaacgcca aaggcgaagc
    29581 tatgccgata gcaccaggcg atggttatac tgtgtggctc ccggtcccgc aggatcttga
    29641 gctcaattac gcgctgctga tgcgtaattt ctccggggaa accacgcgta atccccacgg
    29701 taagtgatta atttcgatta tttttcccgg atggaaaatt cttagaaacc gatcacatac
    29761 agctgcattt attaaggtta tcatccgttt cgctgaaaaa cataacccat aaaatgctag
    29821 ctgtaccagg aaccacctcc ttagcctgtg taatctccct tacacgggct tattttttat
    29881 gcataagccc tatccctggt caccgtcttc cattgaccac atcgatagaa tctcccttca
    29941 tagcacgatg cctttcacgt aacggcatcg tgctcgcaca ggttccggct aagcacaacc
    30001 agaacgcgca tgtttgacgc ttaccaaaaa atattctcac tctccacatt tgaatgtcag
    30061 acgagcgacg ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct gcattttttt
    30121 gccctgagta acacagaaat gggctgcatc attttttact atattttctg caccagatat
    30181 tctgcccctg gctaaagaag cttcggcttc ggtgtagtat tggttatcga gtttacgctg
    30241 aatattactt ttatatgcaa gaccaaattt accgat
//
LOCUS       DAZFWP020000048        30037 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFWP020000048 DAZFWP020000000
VERSION     DAZFWP020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.048"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..1589
                     /gene="rtcR"
                     /locus_tag="ACHK0Z_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             3..1589
                     /gene="rtcR"
                     /locus_tag="ACHK0Z_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU4502017.1"
                     /translation="MAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRLELLH
                     DTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQPEKE
                     DYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSRY
                     NAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKS
                     FLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRS
                     ANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSVFQLISGTVRDLRQLV
                     AEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTEARRA
                     WLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQESRPSA
                     LTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDADRLR
                     KYLARFGLTWEAVQDQHSSS"
     gene            complement(1571..2329)
                     /locus_tag="ACHK0Z_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             complement(1571..2329)
                     /locus_tag="ACHK0Z_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU4502018.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2346..3176)
                     /gene="glpG"
                     /locus_tag="ACHK0Z_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             complement(2346..3176)
                     /gene="glpG"
                     /locus_tag="ACHK0Z_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU4502019.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3221..3547)
                     /gene="glpE"
                     /locus_tag="ACHK0Z_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             complement(3221..3547)
                     /gene="glpE"
                     /locus_tag="ACHK0Z_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU4502020.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3737..5242
                     /gene="glpD"
                     /locus_tag="ACHK0Z_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             3737..5242
                     /gene="glpD"
                     /locus_tag="ACHK0Z_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU4502021.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5294..5899)
                     /locus_tag="ACHK0Z_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             complement(5294..5899)
                     /locus_tag="ACHK0Z_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502022.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5902..6669)
                     /locus_tag="ACHK0Z_RS09970"
                     /old_locus_tag="pgaptmp_001994"
     CDS             complement(5902..6669)
                     /locus_tag="ACHK0Z_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502023.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDETVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(6672..7382)
                     /locus_tag="ACHK0Z_RS09975"
                     /old_locus_tag="pgaptmp_001995"
     CDS             complement(6672..7382)
                     /locus_tag="ACHK0Z_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502024.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7398..8906)
                     /locus_tag="ACHK0Z_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             complement(7398..8906)
                     /locus_tag="ACHK0Z_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4502025.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9031..11478)
                     /gene="glgP"
                     /locus_tag="ACHK0Z_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             complement(9031..11478)
                     /gene="glgP"
                     /locus_tag="ACHK0Z_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU4502026.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11497..12930)
                     /gene="glgA"
                     /locus_tag="ACHK0Z_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             complement(11497..12930)
                     /gene="glgA"
                     /locus_tag="ACHK0Z_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU4502027.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12930..14225)
                     /gene="glgC"
                     /locus_tag="ACHK0Z_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             complement(12930..14225)
                     /gene="glgC"
                     /locus_tag="ACHK0Z_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU4502028.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14243..16216)
                     /gene="glgX"
                     /locus_tag="ACHK0Z_RS10000"
                     /old_locus_tag="pgaptmp_002000"
     CDS             complement(14243..16216)
                     /gene="glgX"
                     /locus_tag="ACHK0Z_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU4502029.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16213..18399)
                     /gene="glgB"
                     /locus_tag="ACHK0Z_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             complement(16213..18399)
                     /gene="glgB"
                     /locus_tag="ACHK0Z_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU4502030.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18672..19775)
                     /gene="asd"
                     /locus_tag="ACHK0Z_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     CDS             complement(18672..19775)
                     /gene="asd"
                     /locus_tag="ACHK0Z_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4502031.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19968..20561
                     /gene="yhgN"
                     /locus_tag="ACHK0Z_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     CDS             19968..20561
                     /gene="yhgN"
                     /locus_tag="ACHK0Z_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU4502032.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20607..21902)
                     /locus_tag="ACHK0Z_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             complement(20607..21902)
                     /locus_tag="ACHK0Z_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGU4502033.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHENDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21809..23905)
                     /locus_tag="ACHK0Z_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             complement(21809..23905)
                     /locus_tag="ACHK0Z_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HGU4502034.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(24045..25385)
                     /gene="gntU"
                     /locus_tag="ACHK0Z_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             complement(24045..25385)
                     /gene="gntU"
                     /locus_tag="ACHK0Z_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4502035.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPIIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25389..25916)
                     /gene="gntK"
                     /locus_tag="ACHK0Z_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             complement(25389..25916)
                     /gene="gntK"
                     /locus_tag="ACHK0Z_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4502036.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(26055..27050)
                     /gene="gntR"
                     /locus_tag="ACHK0Z_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             complement(26055..27050)
                     /gene="gntR"
                     /locus_tag="ACHK0Z_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU4502037.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27274..27969)
                     /gene="yhhW"
                     /locus_tag="ACHK0Z_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             complement(27274..27969)
                     /gene="yhhW"
                     /locus_tag="ACHK0Z_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU4502038.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(28092..29129)
                     /gene="yhhX"
                     /locus_tag="ACHK0Z_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             complement(28092..29129)
                     /gene="yhhX"
                     /locus_tag="ACHK0Z_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4502039.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29461..29949
                     /gene="yhhY"
                     /locus_tag="ACHK0Z_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             29461..29949
                     /gene="yhhY"
                     /locus_tag="ACHK0Z_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4502040.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         7503 a         8060 c         7415 g         7059 t
ORIGIN      
        1 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
       61 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      121 tggaattgtt gcacgacacc cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      181 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      241 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      301 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      361 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      421 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      481 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      541 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      601 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      661 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      721 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      781 aaggcgcgtt taccggggcg cgggaatctc gtgaaggttt attacgcagc gccaacggcg
      841 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
      901 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgttt
      961 ttcagcttat ctccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1021 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1081 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1141 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1201 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1261 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1321 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1381 aaaacatcga cctttttgac aggctgcaac tggaacacgt gatagctctc tgtcgccagg
     1441 caaagtcgct ttccgctgcc ggacgcctgc tttttgatgt ttcgcgccag ggcaaagcca
     1501 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1561 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1621 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1681 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1741 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1801 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     1861 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     1921 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     1981 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2041 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2101 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2161 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2221 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2281 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2341 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2401 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2461 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2521 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2581 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2641 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2701 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2761 aagtaacgcc agaactcaaa tttcagtgtt ggatcgaatg gccaggccag ccataacatc
     2821 acttcctgat cgccgagaat ttgcatggcg ataaacacca ccacgcaggc gatcatcacc
     2881 acccaggtta ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     2941 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3001 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3061 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3121 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3181 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3241 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3301 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3361 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3421 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3481 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3541 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3601 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3661 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3721 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3781 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     3841 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     3901 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     3961 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4021 gcgcccggcg tggatgattc ggattggtct gtttatgtac gatcatctgg gtaaacgcac
     4081 cagcctgccg ggatcaaccg gtttgcgttt tggcgcaaac tcagtgttaa aaccggaaat
     4141 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4201 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4261 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4321 tagctggcaa gcgcgcggcc tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4381 cgaggggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4441 ggtgccgcgt gtgcataccc agaagcaggc ctacattctg caaaacgaag ataaacgtat
     4501 tgtgttcgtg atcccgtgga tggacgaatt ttccatcatc ggcaccaccg acgtagagta
     4561 taaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaactatc tgctgaaagt
     4621 gtataacgcg cactttaaaa aacagcttag ccgtgacgat atcgtctgga cctactccgg
     4681 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4741 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagct
     4801 gaccacctac cgaaaactgg cagaacatgc gctggaaaaa ctgacgccgt attatcaggg
     4861 tattggcccg gcgtggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     4921 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     4981 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5041 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5101 ggatcacgaa tgggtccgcc gcaccgacga tgccctgtgg cgtcgcacaa aacaaggcat
     5161 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5221 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5281 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5341 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5401 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5461 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5521 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5581 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5641 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5701 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5761 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     5821 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     5881 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     5941 gcataaacac tcatcaatta tccagtcgtc tacccagacc tgatgctgtg tgccctgttc
     6001 tggctgcttt ggtgggttct caatcgcctg ccgcgcgcgt tctttcaaga tggattgatt
     6061 gttgaaggcc caggcattga tttgggtggt aatgacgtta tcgatatcgt cctggttatc
     6121 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6181 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6241 catttccagc atatactgta attcgacagt ttcatcgccg gatacccaat cagtcatata
     6301 ctggcaggca aatcgttgca aaattttcca ttcatcctca cgccaatttt ctttactggc
     6361 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6421 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6481 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6541 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6601 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6661 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6721 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6781 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     6841 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     6901 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     6961 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7021 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7081 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7141 gaaaatcccg gccatacatt gctggacgtt aagccaatat ttaatgtgac attatatcga
     7201 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccag ggcctggtta
     7261 atgagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7321 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7381 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7441 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7501 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7561 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcgtcgttc
     7621 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7681 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7741 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7801 cgttgccagg tctggatctc ctgatcgtta ccgccaaccg tgcgccagtt ggtaattgca
     7861 ccactgaaga tgtcacgcac ttgttgttcg gtcagggaat taaccgggtt atctgcattg
     7921 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaaa tcaaagtgac gcccgattcc
     7981 tccgcgcgtt ttttctgccc accggaaggt tgagccacaa agataatatc ggcattacct
     8041 ttaacaatct tgttatatgc ttcgggagta cgagagttcg ccagatattc ccattcgtga
     8101 aaatcttccg gcaaaacgct tagtgcataa aaggcagagg cataaatggg gtacgccgcc
     8161 gttgcgccat caagtcgcgg ccagttttgc gtgaactgaa tttgcggttt accacgcagt
     8221 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcacgc
     8281 acaccgacgc cgggattaag ctgtttatat aaataagcct ggtaaccggc gacaaaacca
     8341 agcatgagaa ggataaacaa gagagaatta cggcaacgta acccggacgt gcccgttaaa
     8401 ggatatctgg tcggccagcc gtaccccagc gaaaaactga tatgcgcgcc aacaggtata
     8461 atgactaacc accagtactc accaccgact aatgaggcta acaaaacgcc cagccagggg
     8521 agcataagat cagccaacca acccgctccc atctggtgaa attgataatc tgccagatgc
     8581 agcgcgatta tccagacccc cagggttaat aatgcgggta ctaaaaaaag acattgtcgc
     8641 cagacggcta tttctctttt ttctggtgca ctatcgtccg cccacactcg ccccgagaaa
     8701 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta aattgttggc
     8761 gtgataaaga tattacaaac ttcaataata ccggcaagtc cgacacccag catggcaata
     8821 accgccgcca aaaactgcgc cagaatgggg atgccgaaaa aagtcattac cagcgaggtc
     8881 aaaatccatt ttctgttttg cattattctt tccattcttt ctgaatggcg aaattatact
     8941 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcgaaa aaaacgggac
     9001 cctttggccc cgttctattt attagtgaaa ttacaatctc accggatcaa tatgccagat
     9061 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
     9121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcatcga ctttatcctg
     9181 acaatcgaca tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag
     9241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
     9301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
     9361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aagataaaga tattgtcagc
     9421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
     9481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccccgcca gcgaaatctg
     9541 ttcagacaga tcggccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
     9601 gaacacgact ttcagcttgt cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
     9661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
     9721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
     9781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
     9841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
     9901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
     9961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
    10021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
    10081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
    10141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
    10201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
    10261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
    10321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
    10381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
    10441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
    10501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
    10561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta
    10621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
    10681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa
    10741 tttaccgagg ttaatttcgc tactggcttg ggcactccac aaacgcaacg tgttggtcgc
    10801 gtcggtatcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
    10861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
    10921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
    10981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
    11041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
    11101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
    11161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
    11221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
    11281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
    11341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
    11401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
    11461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
    11521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
    11581 aaaccgccac agtgaaggac gggaccacag tacaaaagca cgccgaatag cccgtaacag
    11641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
    11701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
    11761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
    11821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
    11881 gcccacctga ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
    11941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
    12001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
    12061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
    12121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
    12181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
    12241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
    12301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
    12361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
    12421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
    12481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
    12541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
    12601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
    12661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
    12721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
    12781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
    12841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
    12901 catctctgaa catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt
    12961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
    13021 tcctctgcgt tttcaccaat caccatgcct tccggaataa cacaagcacg atcgatgacg
    13081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
    13141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
    13201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
    13261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
    13321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
    13381 gcttccagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
    13441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
    13501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
    13561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
    13621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
    13681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
    13741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
    13801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
    13861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
    13921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
    13981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
    14041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
    14101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
    14161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
    14221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
    14281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
    14341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
    14401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
    14461 gttgagcata tcgatttagc caacggacat tgccgtcgcc ttcttcccac cagcgattct
    14521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
    14581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
    14641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
    14701 agagcaacaa cgttgttaac agggcgtgaa tgctgtcgcg ccgccgttca accaggtcaa
    14761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
    14821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
    14881 taaaaccgtc atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt
    14941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
    15001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
    15061 ggaaatttcc cacctgataa ccaccaggag cgatatccca cggttcagca attaacttca
    15121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
    15181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt
    15241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
    15301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
    15361 tacggttatc gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat
    15421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
    15481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
    15541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
    15601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
    15661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg
    15721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
    15781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
    15841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
    15901 ctttaaactc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
    15961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
    16021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
    16081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
    16141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
    16201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt
    16261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
    16321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
    16381 gcccggctgg tttatgccga agcgataatc atgacgcggt accggcgtaa agttactggc
    16441 aacgatgatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
    16501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
    16561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
    16621 atcgccgcct tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg
    16681 ggcaaattcg ttacccatga acagtagttt cttgcccggg aatgcccaca tccagccata
    16741 gtaggcgcgc aggttcgcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
    16801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
    16861 gtagagaatc ccgaaggtca gtttatcgtg atgatactga cgataaaccg ggtcgagctt
    16921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
    16981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
    17041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
    17101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
    17161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
    17221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat
    17281 cagcgtgttc cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt
    17341 gccatcaaat tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag
    17401 aatcacgttc agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
    17461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
    17521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
    17581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg
    17641 ccaggaaccc aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt
    17701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
    17761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
    17821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat
    17881 gccgctctct ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt taaattgccc
    17941 aaccaccgag acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc
    18001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
    18061 taatagccag gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag
    18121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
    18181 aatgacgccg ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc
    18241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
    18301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
    18361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
    18421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
    18481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
    18541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
    18601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
    18661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
    18721 agcagctggt cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc
    18781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
    18841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
    18901 tccacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
    18961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    19021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    19081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    19141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    19201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    19261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    19321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    19381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    19441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    19501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    19561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    19621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    19681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    19741 ataccgcgcc agccgataaa accaacattt ttcataagcg tttttttcct gcaaagatgt
    19801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    19861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    19921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    19981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaaccta cctattttca
    20041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    20101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    20161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    20221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    20281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    20341 tgttgctgtc tcaccagtac ccaaatcaga tgggacatct ggtgattgct ctgctgctgg
    20401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    20461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc
    20521 agatgttcct cgacggcatt agaatgtgga tgaaggggta aggttcaaac aggtgctgca
    20581 cacaaccaga cgcggcacct gttttattac ctgaactgat aatgaccttc gcccaatcgg
    20641 ataacccttc cctcttcttc tgcgcggttc agccacacac taatttgttc gatggttaaa
    20701 ccagtacaag acgacagcct ttcaatactt atcgattcct ttgcaaagat cgccagttcc
    20761 gccacaaaga gttgataaaa atcgacaggc gtatgcgcgg gcattactgc gtaatttgcg
    20821 tcattttcat gcagcgaaaa cagttctggt tgagcgttac ggagtgctct gtcatacatc
    20881 agcccaggaa cgtctgacag tctgatcggc acattctcag cctgttcagc actccatgac
    20941 gccccatttt ctactaatcg tgaattggct gattgcatat cctgatttgg tttaacctgc
    21001 acaggcagcc actgatgttt taatgcctct atcgccccgg ttatcgttcc accggtcatt
    21061 cccgcacgta caaccattgc gctttcagca aggcaataaa tatatttatt tcgcgccatc
    21121 gcattgccga cggtgaaacg cacctctggg taaaacggcg aaatcaacac caggttgcct
    21181 gctataagcc cttcacgcca tttcactaac gtactcgttt ttaacaagct atcagctaat
    21241 acgccaacgg cagttccccc ggcctccagt gccgacgcca ttgcacattc atcaatacct
    21301 cgcgcaccac cagagataac gcaaatcccc tgttgggcca gtttcgcggc cagttgttgg
    21361 gtatagcgca aatcgtcagt cggagcatct cgcgagccaa caatcgccat accttccgct
    21421 tgcagtaatg ctttattccc gcagccaaat aaaacgggag gtgcatccgt tcgcaatcgg
    21481 tttttcaagc gaacgggata atcagcatct cctcgggtta tgatccataa accggcgcgt
    21541 tgccacttat ctaccgccaa cgccagacta tgaccgcgcg ccagcaagcc aagaatacgc
    21601 tcctgagaaa tacgcggatc gctccagcct gtaagtttct cttgcggttg cggcaccaga
    21661 agctcggcgg gactgatacg ttggtgcttc agccataatg caaacttccc ccattcacta
    21721 ttactaagag gtttatgctc actcgccgcc gcgcgagaaa aatcgctggt tagcaggaga
    21781 gtcgcttgtg cattggctga aagattcatc aattttttac cgaggtagaa gcaagggcaa
    21841 gaggataaac cgtcgggcaa cctgcctggc gtaacagtgc tgtcagaact gtcagcgtcc
    21901 atgcagaatc cacgatatcg tcaaccagca acgccggacc tggcatcaaa ggaggggtaa
    21961 tcacaaacgc cccgtcgaga ttttgacact ggtgaaaacg gttttgctgc attttctgcg
    22021 gtggattgtc cacgactttt tcaatggcat caataaaagg taagccaagt ttcgccgcca
    22081 gtcgccgggc aaaatcagga accaggtcga ggtgccttaa tgaaggaacg cagcataccc
    22141 aggttggttc aggatgcgga ttccagcgtt ggcgaaccat ttccgcacat gcctccacca
    22201 gctcatcact gaatcgcccc gtttttttct cctgtgctac ctgctttccc catccggaat
    22261 ctccccaacg agaaaggact cgccccgtag atccttgtaa acccgcaggc aagttccctt
    22321 taaatccgta ttgcgtaaaa gctccagatg ctacctgttt attgagattt aatggcaggt
    22381 cagcgtgttt aataaataaa tttgccgcat ggagtaagcc gctatcaata tcgggcgaca
    22441 acaacggatg ctgaagacaa ctgctacatt taccacaacg ttcggcactt ggctcatcga
    22501 gtgcgcggcg cagaaattgc attttgcact ccttggtcgt gatatagctt tcaacatctg
    22561 ccagctcatt ttttctctga ttcatcaaat ggttaattcg ttcatggtca ggagagaaac
    22621 tgacgatagt tctgcgccat aatttctcgg tatacaccac aggcgatgga ttttcagcta
    22681 ccaataattt cagtgctttt tctatttgcc cataacggag attcgtccgt tgttcaatgc
    22741 ctcgtagggt aagaccgtca ttctcgctaa gtacgttgag tatttcatga atttgcgcct
    22801 ccgcaggaaa ggcgctttca cggaagaatt tatgaatagc gcggtcttca ccaccacaaa
    22861 gcaatatgcc aacagcggaa tctatagcac gcccggcacg ccctacctgt tggtagtaac
    22921 cgacaataga ccccggcatc tggtaatgaa taacaaaccc caaatccggt ttgtcaaagc
    22981 ccattcccag cgccgtcgtc gcgaccagaa ctttaatttt atttgccagc aacgcctgct
    23041 ccagatattc cctggcagta ttagaatctt ccgcgccttc acaagtcacg ccgctgtagt
    23101 aagcaaatgc actgatacca tttgtcctta accactcggc gacaaggtcg gcatcgcgag
    23161 tagtcagggt ataaacaatt cccgatttgg aaaactgagg gataaccgtt gctaaccatg
    23221 ccagacggga tgactgttca cctaagacta aggcatcaag cgccaaactt tcgcgagcca
    23281 gcgttccacg ctgaatcaca atgtcaccca attgctgacg gatatcctca acgacacggt
    23341 tattcgctgt cgcggttgta cccagaactg gggtattcgc aggcagttgg cgcaaaatat
    23401 ctaatatacg tcggtaatcc gggcgaaaat cgtggcccca atcagagatg cagtgcgcct
    23461 cgtcgaccac aagcaagcca atacgatcgg ctataggata taaaaccgtt tcgataaagt
    23521 cttggtttgc caaacgttcc ggggaaacca gcaagcaatc gacctcacct tgcagcaact
    23581 tatcgctaat acgctgccat tcttccctgt ttgtagaatt caacgtttca gccgtaatac
    23641 ctaaccgttc agcggcggca acctggttac gcatcagggc aagcaaagga gagatgataa
    23701 ttgtcgggcc agcaccgcga tcgcggaaga ttttactggc gatgaagtac acggcacttt
    23761 tcccccagcc agtgcgttgg acaaccagca gttttcgtcg ttggttcacc accgcgtcta
    23821 tggcttccca ttgcccttcc ctgaaagtag cggtggtatt tcctaacatc cgctgtaaaa
    23881 gcaattcagc tgcgtgcttt tccatcttta tctcctgcga tctttaggca gtgattagaa
    23941 cggattatta gcaggtaaat ttgcgagagg gatcaaaaag atgaagagag gagataagag
    24001 aaatgaatta ctgcgagcaa gctcgcagta atttgtcgca gtaattaact caacagctgg
    24061 aacgcaatca tcccaacgat ggcaccgaca gtgccgagga tggtttccat catggtccag
    24121 gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca gaaaccggcg
    24181 tcgttaacgt ggctgacaac aatcgaacca ccagcgatac aaatcgacag cgccgccatt
    24241 tgcgcaccgg agtagttcag ttgttcaata atcggcatca ccagtcctac cgccgttaaa
    24301 caggctacgg tggcagaacc ctgaatgatg cgcactgcag ctgccagcac gaagcaggtg
    24361 atagcaatcg gcaggcccat gccggttaac gcttcgccca gtgccggacc tacgccagag
    24421 tcaaccagca cctgtttgaa cacgccgcct gcaccaatca ccagcagaat gatccccgcc
    24481 ggttgcagcg cgtgaccgca aatctccatc actttgtctt ttggcatgcc ctgacgcatt
    24541 gccaggccat aaatcgctac cagacaagca accagaatcg cggtaaacgg atgaccaata
    24601 aactcgaacc attcgtaagc ggtagagcct tctggcacaa aacgcgcggc aatggttttc
    24661 agccccacca gcaccagcgg caacaggatc agcgacaggc tgaatccgaa agatggcatt
    24721 ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa tatgcaactc cacgtagcgg
    24781 ctgatgaaat taccccacag cggcccggca ataatcattc ccggaattgc cgcacacagg
    24841 ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg atgccagcag cattggcgct
    24901 ggccctggca ccaggaacgc cgccgctgcc gccacgcctg cgaataatgg gattaccagc
    24961 ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacgctaat cagcagaaca
    25021 atcgccactt caaagaacag cggtagcgca cagaccagcc ccgcaaggcc gatggcataa
    25081 tgcgcgcggc tgtgaccgaa ggatttaagc attttgacgg caatctgatc gactgcgccg
    25141 gtttcatgta agatcttgcc aaacatagct cccagggcga caaccaccgc caggaagccg
    25201 agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg cataccggaa
    25261 aaaaggccag cccccatgga caccaccatc aaagccagga aagcgtgcat acgcgccttc
    25321 atgactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag cgttaatgta
    25381 gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac accttccagc
    25441 ggttgatcga tatccaccac cagtacatca gtttcgtccg cacccggctc ctgcagcgtt
    25501 tcaaactgcg tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc tttcaggcgg
    25561 ctttcaatca cgtcaaaatc gcctttcagg tagatgaaag agagattcgg attaccttca
    25621 cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga cgatcagtga cactttatta
    25681 gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca accacggttt gcgatcgtcg
    25741 tcattcagcg gttcgccaga cgccattttt tcgatattgc gccgtggatg gaggaaatcg
    25801 ccatcaagaa acgcggcatg aagttgatgc gccacttcac tggcgaccgc agatttgccg
    25861 ctgcctgaga cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt gctcaaagtg
    25921 gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt aacattgtcc
    25981 ggccggacaa tatcagaagc aatatccatc cgtgccttaa gtgtataagt gtgagctact
    26041 tcaaatttgt gggcttaaat agatccgccc ggtgacaagg tgaaacctaa atctaacatt
    26101 ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc aatactgccc
    26161 atccgctcac gcggcgtcag cacgctcgcc agtcgtggct ccatcacctg accaatgtca
    26221 tgaccgtgga aaccggcaat cgccatatcg tcaggaactt ttaaccccag acgctgacat
    26281 tcaaacgccg cgccgaccgc cagatcatca ttagtacaga acacgccatc cagctgcgga
    26341 tattcccgcc gcgcctggcg aatcagttca ataccggaag agtaagaaga agattgctca
    26401 accatcacgc tatacggcac caggcctgca tccagcatcg cctgttcgta tcccttctgt
    26461 ttgatgatag tacgttcgtc gagacgtgcg ccgagatagg caatgtggcg atgcccgcgc
    26521 gcaataatgg cggtagtcat ctggcgtgcc gcttcaaagt tatcaaaacc gacggcgata
    26581 tcgaggcacg gcgactggct gtccatcaac tccaccaccg ggatccccgc cacttcaatc
    26641 atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga tcaggccgtc gatattccag
    26701 gacagcatgg attcgagacg ttcttgctcc atttccggtt tataaccgta gtgcgccagc
    26761 atcgtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac ttccgcgaaa
    26821 acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtagc gttagagagg
    26881 atatcgggcg cacgattggg aatatagccc agttcatcaa gagcggcggc aatcttgccg
    26941 cgtagagcga cggaaacctg ctccgggtta cgcaaaaagc ggctgaccgt cattttggtc
    27001 acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat cgtcctgaag
    27061 gtacaaaaga gatagattct cttagtttaa cacggacgca tcagaacctt cccggggaaa
    27121 acagtgcttg tttacaatta ttagatatac actaaatcaa ttgcatcgca ttgtgctaac
    27181 gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc ggagcgagcg aggaagcctg
    27241 caaaaaggca ggcttcaaag atgccgttga gttttaaacc ggcggcagat cgaacagtaa
    27301 cacttcgctg tcgctatccg catggatgga gattgcctgc tcatcccaga ttgccagacc
    27361 atcgctggtc gaggctttca cgccgttaat ggtgacattg cctttcacca cctggatcca
    27421 gacgcggcgt tcagcggcaa tctgatgcac cgactgctca tctttcagca acgcccaacg
    27481 atacagttcc atatcctgat gcactttcag cgaaccatct cgcgcatccg gcgagagcac
    27541 cagctgtttg ccctgcacgg catcgaagcg acgctgttca taacgcggcg taatgccatt
    27601 ttcttcaggc atgatccaga tctgatacag atgcagacgc tcggtgctgc ttgggttgta
    27661 ctctgagtga cgaatacccg tacccgcact cataatctgg aactcacccg ccggaacctg
    27721 ctctttattg cccatgctgt cctgatgctc aacagtacct tccagcacgt aggtcaaaat
    27781 ttccatatct ttatgcgggt gagtgccgaa gccctgccct gcttcaatca cgtcgtcgtt
    27841 aatcacgcgc agcgcggaaa agcccataaa gttcggatcg taatagttgg caaaagagaa
    27901 agtatgccag gagtccagcc agccatgatt tgcatgacca cgttcatttg ctttgcgtaa
    27961 gtagatcatt tgtattcgcc ccatgaatga tttcgatggg cttagtgtgg acccgatctg
    28021 cctgggatga tagagggtga aaattgaccc ctctgttcaa aaaaattgaa ctattcgagg
    28081 ggccatcaaa cttacttagc gagagttact gtggagggag aggcttgctc aaatccgcgt
    28141 tcaaggattt ccagattcgt aagaacttca gattccttga cgtaatttgg cgcaccgtgg
    28201 gtgatggttt gatacaacgc atcataaacg cgcccgtaat cgcccatctc cggcttcatc
    28261 tcttctctga ccgtcacgcc ctcgtcattg acatactcca gcacaccgac cgaatcatcc
    28321 gctgcgaatc ccggttcgcc cggcataata ttagccttca ggctggtttc ctgctggtcg
    28381 ataccgtatt taataaacga acctttctta ccgtgaacga taaatttcgg ataatcgatt
    28441 ttcaccagat ggctggtttt gacgatggct ttcaggtcgc cataaaacag ttgcgcttca
    28501 aaggtgtcgt caggattggc tttattacgc aggctgcgga tgtcataagc gacgtgatcc
    28561 gggcgaccga acagagaaat aatctggtcc atcgtatgca caccaaggcc ataaaacgcg
    28621 ccatcctgcg gcagcccagg tttggtctct gccaccgggc ggtaatagtc aaaatggctt
    28681 tccacttcaa caatctctcc caacttgcca ctttcaatcg cttttttcgc tgtcaggaag
    28741 caggagtcaa agcgacgatt ctgatacggc gtgacggtca gccctttgct tttcgccaac
    28801 gcaaacagct ccttcgcctg ggcaagcgtc ggggtgaacg gtttttcgac cagcacattt
    28861 ttcccggctt ccagcgcgcg tttcgcgtac tcgaaatggc tgtccgcgtg ggtgcagaca
    28921 acaaccagct taacatcggg atcgtttagt acttcgtcga gatcgctggt gaaatggata
    28981 tgggaataaa tgggagcctg ttcttccggc ttcgcatggc gacgaaaaat atgcgcgaca
    29041 tgccagctat ccttgcggtt aagtacatac ggcagatggt aacgggtggt gcttttgccg
    29101 aagccaataa aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg gttatttaca
    29161 ccttagcgca aagcggacgt ggttcctact ggagtgcgca taacgaacac aagcactccc
    29221 gtggataaat tgagaacgaa agatcaaaaa aaagccagca cccggctggc taagtaatac
    29281 tggaagcaat gtgagcaatg tcgtgctttc aggttctccg cgagggtctt cctgatcgcg
    29341 agacaataat aatcattctc attcgcactt gtccaacact ttttgcaaaa aaatgcattt
    29401 gactcgcatt tgaaagtcaa tgatgttgaa agggacattt accccaaaga ggacaaagga
    29461 atgagtgaga tagtaatacg ccacgcagaa acacgggatt acgaggccat caggcagatt
    29521 cacgcccagc cggaggtgta ttgcaacaca ctacaggtgc ctcatccttc cgatcatatg
    29581 tggcaggagc gactcgccga tcgtcccggc atcaagcaac tcgtcgcctg tattgatgga
    29641 gacgtcgtgg gccatctcac cattgacgtg caacagcgcc cacgccgcag tcatgttgcc
    29701 gattttggta tctgtgtcga ctctcgttgg aagaaccgcg gcgtcgccag cgccctgatg
    29761 cgagagatga ttgaaatgtg cgacaactgg ttgcgggtag atcgcattga actaaccgtg
    29821 tttgtcgata acgccccggc aattaaggtc tataaaaaat acggctttga aattgaaggg
    29881 actggtaaga agtacgcatt gcgtaatggt gaatatgtcg atgcatatta tatggcgcgg
    29941 gtgaagtaag atagtgccct ttttctgaga tggaaaaagg gtgtcattca aaatcgacat
    30001 accttccttt aaggtattta tttgcccaat acatata
//
LOCUS       DAZFWP020000049        30012 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFWP020000049 DAZFWP020000000
VERSION     DAZFWP020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.049"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHK0Z_RS10060"
                     /old_locus_tag="pgaptmp_002012"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHK0Z_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU4502041.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHK0Z_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHK0Z_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4502042.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHK0Z_RS10070"
                     /old_locus_tag="pgaptmp_002014"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHK0Z_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU4502043.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHK0Z_RS10075"
                     /old_locus_tag="pgaptmp_002015"
     CDS             complement(1087..1581)
                     /locus_tag="ACHK0Z_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU4502044.1"
                     /translation="MRILVLGGGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHK0Z_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHK0Z_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU4502045.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHK0Z_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHK0Z_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU4502046.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHK0Z_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHK0Z_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU4502047.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSDTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHK0Z_RS10095"
                     /old_locus_tag="pgaptmp_002019"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHK0Z_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU4502048.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHK0Z_RS10100"
                     /old_locus_tag="pgaptmp_002020"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHK0Z_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU4502049.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHK0Z_RS10105"
                     /old_locus_tag="pgaptmp_002021"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHK0Z_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU4502050.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAVGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9234
                     /gene="gpr"
                     /locus_tag="ACHK0Z_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /pseudo
     CDS             8195..9234
                     /gene="gpr"
                     /locus_tag="ACHK0Z_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(9274..9768)
                     /locus_tag="ACHK0Z_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     CDS             complement(9274..9768)
                     /locus_tag="ACHK0Z_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU4502051.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9959..10843
                     /gene="yghA"
                     /locus_tag="ACHK0Z_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     CDS             9959..10843
                     /gene="yghA"
                     /locus_tag="ACHK0Z_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4502052.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11115..11540)
                     /gene="exbD"
                     /locus_tag="ACHK0Z_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     CDS             complement(11115..11540)
                     /gene="exbD"
                     /locus_tag="ACHK0Z_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU4502053.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11547..12281)
                     /gene="exbB"
                     /locus_tag="ACHK0Z_RS10130"
                     /old_locus_tag="pgaptmp_002026"
     CDS             complement(11547..12281)
                     /gene="exbB"
                     /locus_tag="ACHK0Z_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4502054.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12533..13720
                     /gene="metC"
                     /locus_tag="ACHK0Z_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             12533..13720
                     /gene="metC"
                     /locus_tag="ACHK0Z_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU4502055.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13860..14519
                     /gene="yghB"
                     /locus_tag="ACHK0Z_RS10140"
                     /old_locus_tag="pgaptmp_002028"
     CDS             13860..14519
                     /gene="yghB"
                     /locus_tag="ACHK0Z_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU4502056.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14559..15458)
                     /gene="yqhC"
                     /locus_tag="ACHK0Z_RS10145"
                     /old_locus_tag="pgaptmp_002029"
     CDS             complement(14559..15458)
                     /gene="yqhC"
                     /locus_tag="ACHK0Z_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU4502057.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQSSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15652..16815
                     /gene="yqhD"
                     /locus_tag="ACHK0Z_RS10150"
                     /old_locus_tag="pgaptmp_002030"
     CDS             15652..16815
                     /gene="yqhD"
                     /locus_tag="ACHK0Z_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU4502058.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAETRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16920..17747
                     /gene="dkgA"
                     /locus_tag="ACHK0Z_RS10155"
                     /old_locus_tag="pgaptmp_002031"
     CDS             16920..17747
                     /gene="dkgA"
                     /locus_tag="ACHK0Z_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU4502059.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17950..18876
                     /locus_tag="ACHK0Z_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             17950..18876
                     /locus_tag="ACHK0Z_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGU4502060.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGEIGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18927..19184
                     /gene="yqhH"
                     /locus_tag="ACHK0Z_RS10165"
                     /old_locus_tag="pgaptmp_002033"
     CDS             18927..19184
                     /gene="yqhH"
                     /locus_tag="ACHK0Z_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU4502061.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19227..21446)
                     /locus_tag="ACHK0Z_RS10170"
                     /old_locus_tag="pgaptmp_002034"
     CDS             complement(19227..21446)
                     /locus_tag="ACHK0Z_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU4502062.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21557..22969)
                     /gene="ftsP"
                     /locus_tag="ACHK0Z_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             complement(21557..22969)
                     /gene="ftsP"
                     /locus_tag="ACHK0Z_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU4502063.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23044..23781)
                     /gene="plsC"
                     /locus_tag="ACHK0Z_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /gene_synonym="parF"
     CDS             complement(23044..23781)
                     /gene="plsC"
                     /locus_tag="ACHK0Z_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU4502064.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24014..26272)
                     /gene="parC"
                     /locus_tag="ACHK0Z_RS10185"
                     /old_locus_tag="pgaptmp_002037"
     CDS             complement(24014..26272)
                     /gene="parC"
                     /locus_tag="ACHK0Z_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU4502065.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26818..27300)
                     /locus_tag="ACHK0Z_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     CDS             complement(26818..27300)
                     /locus_tag="ACHK0Z_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU4502066.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27353..27745)
                     /gene="ygiW"
                     /locus_tag="ACHK0Z_RS10195"
                     /old_locus_tag="pgaptmp_002039"
     CDS             complement(27353..27745)
                     /gene="ygiW"
                     /locus_tag="ACHK0Z_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU4502067.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27897..28556
                     /gene="qseB"
                     /locus_tag="ACHK0Z_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     CDS             27897..28556
                     /gene="qseB"
                     /locus_tag="ACHK0Z_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU4502068.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28553..29902
                     /gene="qseC"
                     /locus_tag="ACHK0Z_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     CDS             28553..29902
                     /gene="qseC"
                     /locus_tag="ACHK0Z_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU4502069.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
BASE COUNT         7368 a         8035 c         7606 g         7003 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcaacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgcc
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcagca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggta
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcgcagc agtgaacggt acgaccaata atacggccca gcgtagagtg cagttgcgcc
     2041 acttccagcg tgttaccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc gggctgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat atccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgtcg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctac cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa aattccgctt
     8761 ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag cggcctgctg
     8821 gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc tcagggattg
     8881 ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgtatcg tgaagggaat
     8941 aaagttcgtg gtctgacacc gaaaatgctt accgaagcca acctcaacag cctgcgctta
     9001 ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt aagttggttg
     9061 ctgaaagatg agcgagtgac gtcggtattg attggtgcca gccgcgcgga gcaacttgag
     9121 gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc gcagattgat
     9181 cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa atgacctgtt
     9241 aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag tcagtcggtc
     9301 aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga cgtaccacat
     9361 cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaggt gaatggaaga
     9421 aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca ttttccccag
     9481 ccagttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat tctcataacc
     9541 ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca gcgacagcaa
     9601 cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt gaataatctc
     9661 ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt acacgggggc
     9721 aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac attcctgtcg
     9781 tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa cagcaaatca
     9841 acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc tggttccctt
     9901 atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga gaaaaacgat
     9961 gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca aacagaaaca
    10021 accgacgcca ggcatccagg cgaagatgac accggtaccg gattgcggcg agaaaaccta
    10081 tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg attccggaat
    10141 aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga tcagttatct
    10201 tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat gcggacgcaa
    10261 agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc tggttcacga
    10321 agcgcataag gcgttaggcg ggctggatat tatggcgctg gtcgccggga aacaggttgc
    10381 cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg ccattaacgt
    10441 tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag gtgcaagtat
    10501 catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg actatgcggc
    10561 tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg cggagaaagg
    10621 tattcgggtg aatattgtcg cgccaggccc gatctggaca gcactgcaaa tttccggcgg
    10681 acaaacgcag gataagatcc cgcagtttgg tcagcaaacg ccgatgaagc gtgcggggca
    10741 accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga gctacgtcac
    10801 cgcagaagtg cacggtgtgt gcggcggcga gcatttaggt taaaaaaatg cccggttgtg
    10861 aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa acgccttatc
    10921 cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc tgataagcgt
    10981 agcgcatcag gcattgagca cctaatgccg gatgcgtcgt gaacgcctta tccggcctac
    11041 aaacgcacga agattcaacc tgttgcaata gattttgtag gcctgatgag cgtagcgcat
    11101 caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc tatcttcagg
    11161 taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc gacggttttg
    11221 tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt caacgccgta
    11281 atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc tgccttcacc
    11341 gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc aggcaagttc
    11401 accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag cagaaccaac
    11461 atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc gtcgaggttt
    11521 tcgttaagat gcattgccat cggatattat cctgcgcgta atttttgtgc gacacgaacc
    11581 ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa caatacctgc
    11641 gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa tacgttatag
    11701 ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag agcttctgcg
    11761 atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc aataaagctg
    11821 ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc gccgatggtt
    11881 gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg acgctccaga
    11941 cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga cagttccagc
    12001 tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa atcagcggcg
    12061 atatcgttgg cctggtttaa ggaacgcgct tcagccagca gttgctgctc gcgcttaagg
    12121 cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca ggtgactacg
    12181 gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgtg ctgatacata
    12241 ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct ccaggacgca
    12301 agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt cgttctcatt
    12361 actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca gtaaaaaagt
    12421 caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg atgcgcccgc
    12481 gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg agatggcgga
    12541 caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca ctctcggcgc
    12601 ggtaaatagc gtgattcagc gcgcttcttc gctggtcttt gacagtgtgg aagccaaaaa
    12661 gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg gaacgttaac
    12721 ccatttctcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct gcgtgctatt
    12781 tccctgcggg gcggcagcgg ttgctaattc cattcttgct tttgtcgaac agggcgatca
    12841 tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca aaatcctcag
    12901 caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata tcgttaagca
    12961 tctgcagcca aacactaaaa tcgtgtttct ggaatcgcca ggctccatca ccatggaagt
    13021 ccacgacgtt ccggcgattg ttgccgccgt gcgcagtgtg gtcccggatg ccatcattat
    13081 gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg gcatcgatgt
    13141 ttccattcag gccgctacca aatatctggt tgggcattca gatgcgatga ttggcactgc
    13201 cgtgtgcaat gcccgttgct gggagcagct gcgggagaat gcctatctga tgggccagat
    13261 ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag gtgtgcgttt
    13321 gcgtcaacat catgaaagca gtctgaaagt ggctgaatgg ctggcagaac atccgcaagt
    13381 tgcgcgagtt aaccaccctg ctctgcctgg cagtaaaggc cacgaattct ggaaacgaga
    13441 ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca gtaatgaaga
    13501 gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt ggggcgggta
    13561 tgaatcgttg atcctggcga atcaaccaga acatatcgcc gccattcgcc cacaaggcga
    13621 gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg tcgacgatct
    13681 gattgccgat ctggacgccg gttttgcgcg aattgtataa cattgccact tttggacaat
    13741 tttgcagaca tttaattgtg aaaagtctta aattgttgcg tccgggatca aggcgtcccg
    13801 gacgaatcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca ggaaagtcca
    13861 tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc gcgctggcgg
    13921 atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt ttagaaaacg
    13981 gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca ggcgcattga
    14041 ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc gcagcaagtc
    14101 tgggctgctg gctaagttat attcaggggc gctggttagg gaataccaaa acggtgaaag
    14161 gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg tttgaccgcc
    14221 acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg ctgctgccaa
    14281 ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac tggttaagtg
    14341 gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg attccgttcg
    14401 ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt gccttgttaa
    14461 ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt aacgcctgac
    14521 gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg catcgcccgc
    14581 attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc acggctaaat
    14641 tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tagccttcat gccgtcatgg
    14701 atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa cggcgaggtg
    14761 ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc ttctgccgcc
    14821 agttgctcga cgctcaggtt ttcggtgtat ttattctcaa tccgtttcag cacgcggcta
    14881 atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc gcaaggtccg
    14941 gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg cgcatccagt
    15001 ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc tgataacgtg
    15061 gcggagttaa tcccgctggc tgccatcgac gactggaaat gctcatcttc gccaatgtcc
    15121 atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc cagcggcacc
    15181 tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag caggtattca
    15241 ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc ggaaaagaga
    15301 attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc gccatacaac
    15361 aaacgcacat cgggcaacag ttctgacaaa ctattttctt tatttttcag tttattaact
    15421 ttatccgcca gcaagcggca aatctcttca cgtttcatat cgcgtaattt cttaggaata
    15481 atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg gagaaatagg
    15541 caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca atgaaaaaca
    15601 tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt aatgaacaac
    15661 tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc tggtttacgc
    15721 gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag cgtgaaaaaa
    15781 accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct ggaatttggc
    15841 ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact ggttcgcgaa
    15901 cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg caccaaattt
    15961 atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct gcaaacgggc
    16021 ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc agcaaccggt
    16081 tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa gcaggcgttc
    16141 cattctgccc atgttcagcc ggtatttgcc gtgctcgatc cggtttatac ctacaccctg
    16201 ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt ggaacagtat
    16261 gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat tttgctgacg
    16321 ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt gcgcgccaac
    16381 gtcatgtggg cggcgactca ggcgctgaac ggtttgattg gcgctggcgt accgcaggac
    16441 tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga tcacgcgcaa
    16501 acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa gcgcgctaag
    16561 ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgatga tgagcgtatt
    16621 gacgccgcga ttgccgaaac ccgcaatttc tttgagcaat taggcgtgcc gacccacctc
    16681 tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact ggaagagcac
    16741 ggcatgaccc aactgggcga aaatcatgac attacgctgg atgtcagccg ccgtatatac
    16801 gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt tcgtccagac
    16861 ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag gaggaacgta
    16921 tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag ttgggactgg
    16981 gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg ttagaagtgg
    17041 gttatcgctc gattgatacc gccgcggcct ataagaacga agaaggtgtc ggcaaagccc
    17101 tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg tggaacgacg
    17161 accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactacag cttgattata
    17221 tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc gaagcatgga
    17281 aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg tgcaacttcc
    17341 agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg ataaaccaga
    17401 tcgaacttca cccgctgatg caacaacgcc agctacatgc ctggaacgcg acacacaaaa
    17461 tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc gatcagaaag
    17521 tcattcgcga tctggcagat aaatacggca aaactccggc gcagattgtt atccgctggc
    17581 atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgc attgtcgaaa
    17641 actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcgaaatt gcaaaactcg
    17701 atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacat gcaaattctc
    17761 ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc cctcgcgtcc
    17821 cgccgcccgt tgttactctt ccttgttcag gaatgccaaa tataaggaca tcatcatgca
    17881 gagccggaag ctcttaaaag aacaactcat ctatatccgg gataaacgca acggagaggt
    17941 gaaaaacaga tgaaaataat acttctgttt ttagcagccc tggcaagttt taccgtacac
    18001 gcacagcccc cctcactgac cgtagaacaa acagtccggc atatttatca gaactataaa
    18061 tcagatgcca ctgcccctta ttttggtgaa atcggagagc gggcgataac ttctgcgcgt
    18121 attcaacagg cgcttaccct gaacgacaat cttacgctgc cgggcaatat tggctggctg
    18181 gattatgatc cggtttgtga ttgtcaggat tttggcgatc tggtgctaga aagcgttgcg
    18241 ataacccaaa ctgacgccga tcatgccgat gccgttgtgc gctttcgtat ctttaaagat
    18301 gataaagaaa agaccacgca gacactgaaa atggtggcgg aaaatggtcg ttgggtcatt
    18361 gacgatattg tcagcaatca tggcagcgtc ttacaagcag ttaatagcga gaatgaaaaa
    18421 acgctggccg ctttagcttc gttgcaaaaa gaacagccgg aagcctttgt tgccgaactc
    18481 tttgaacata ttgctgatta tagctggccg tggacgtggg tggtttccga ctcttaccgc
    18541 caggcggtta atgccttcta taaaaccacc ttcaagacgg ccaataatcc cgatgaagat
    18601 atgcaaatag aacggcaatt tatttacgac aatccgatct gttttggcga agagtcgcta
    18661 ttttcacgcg ttgatgaaat tcgagtcctg gagaaaaccg ccgattccgc ccgtattcat
    18721 gttcgtttta cgctgaccaa tggcaacaac gaagagcaag aactggtttt acagcggcgc
    18781 gaaggcaagt gggaaatcgc tgattttatc cgcccgaaca gcggcagcct acttaagcag
    18841 attgaggcaa aaaccgccgc cagattaaag caatgagctg aattaaataa caattagccg
    18901 gaacaataaa taaaagggaa cactatatga aaacgatttt caccgtggga gctgttgttc
    18961 tggcaacctg cttgctcagt ggctgcgtca atgagcaaaa ggtcaatcag ctggcgagca
    19021 atgtgcaaac attaaatgcc aaaatcgccc ggcttgagca ggatatgaaa gcactacgcc
    19081 cacaaatcta tgctgccaaa tccgaagcta acagagccaa tacgcgtctt gatgctcagg
    19141 actattttga ttgcctgcgc tgcttgcgta tgtacgcaga atgataaaaa aatccccggc
    19201 agcatgtcag ttgccgggga ttttttttaa cgtccaaccg ccgctttagg gcgtttcttc
    19261 gcaccagcat tcaccggacg agattgcgta gacgacgctt tttttgccgt agcaggcgtc
    19321 tgacgctggg tcgccatcgg cgtatgtttc gtcaacgccg gacgggtatt gcggttctgg
    19381 cgacgagctt cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg
    19441 ccaatcagat gctttttgcc catcgcttcc agcgcctggc ggattaacgg ccagtttgcc
    19501 ggatcgtggt aacgcaacaa cgctttatgc aaacgacgct gtttgtcgcc cttcggtacg
    19561 aagacgtctt cgctcttata accaatcttc gccagcgggt ttttcccggt gtaatacatg
    19621 gtggttgagt tcgccagcgg cgatgggtag aagttctgta cctggtcgag acggaaacga
    19681 tgctttttca gccacagcgc cagattcacc atatcttcat cacgcgtacc ggggtgcgcg
    19741 gagatgaaat aggggatcag atactgctct ttacctgcct gttttgagta agtatcgaac
    19801 agctctttaa agcggtcata gctgcccatg cccggcttca tcatcttcga taacggccct
    19861 tcttcggtat gttccggggc aatcttcaga taaccgccga cgtgatgggt cgccaactct
    19921 ttgatatagc gcggatcttc cacggcgatg tcataacgta cgcctgaggc gatgaggatt
    19981 tttttaatgc ctttcagatc acgcgcacgg cgatagaggt tgatcgtcgg ttcgtggtta
    20041 gtgtccatgt gcggacaaat atccggataa acgcacgaca aacggcgaca agtttgttca
    20101 gcgcgtggcg atttgcagcg caacatatac atgttggcag ttggcccacc gagatcggaa
    20161 atcacgcccg taaaacctgg aacggtgtcg cggatcgctt cgatctcatt aatgatcgaa
    20221 tcttccgaac ggctctgaat aatgcgccct tcgtgctcgg taatagaaca gaaagaacag
    20281 ccgccaaagc agccgcgcat aatgttgacc gaaaaacgga tcatttcgta agccggaata
    20341 cgggcattgc cataggccgg atgtggcacg cgcttgtacg gcagcgcaaa aacgctgtcc
    20401 atctcttcgg tagaaagcgg gatagcaggc gggttgatcc acacatagcg gtcaccgtgt
    20461 ttttgcatca atgcgcgggc gcagcctggg ttggtttcgt ggtgcagaat gcgcgaagca
    20521 tgggcgtaca gcactttatc gcccttcact ttctcgaaag aaggcagcaa cacgtaggtt
    20581 ttttcccacg gtttcgggcg cggtggctgc acggttacgg ctttggcttc ctgctttttc
    20641 ggtgccaccg gtttgttatc cgcgcacggc aaatcttcac catacggatg cgggattggg
    20701 tcgatttttc caggggtatc aagacgggtg gaatccacgc cgctccagcc aggcagcgcc
    20761 tctttcacga taatcgcggt attacgcaca tcgcggattt cactaatcgg ctcgcccatc
    20821 gccagacggt gcgccacctc caccagcgga cgctcaccgt taccaaacat cagcatgtcg
    20881 gctttcgaat ccaccagcac ggaacggcgc acggtatcgg accagtaatc ataatgtgcg
    20941 gtacggcgca gactagcctc aataccgccg aggatcaccg gtacatcttt ccacgcctct
    21001 ttacaacgct gggtataaac cagtgtggcg cgatccgggc gcttacccgc gacgttatcc
    21061 ggcgtgtagg catcgtcatg acgtaaacgg cgatcggcgg tataacggtt gatcatcgaa
    21121 tccatgttgc cagcagtaac accgaaaaac agattcggtt tacccagacg catgaaatcg
    21181 tctttgctgc tccagtctgg ctgggcgatg atcccgacgc gaaagccctg tgcttccagc
    21241 atacgaccgc aaatcgccat cccgaagctt gggtgatcga catacgcgtc gccagtaacc
    21301 aaaatgatgt cgcagctatc ccagccaagt tgatccatct cttcacgaga catcggcaaa
    21361 aacggtgccg gtccaaaaca ggcggcccag tactgcggcc aggagaacag gtcgcgatcc
    21421 ggttggatca gggagataga gctcattttg cttccagaaa tgataaaaaa ataatcaaag
    21481 gccggggatt ataagccgga acgaaagaga aatcgaaagg tattccatac tcgccctcct
    21541 cgggcgagta tgaagattac ggtaccggat tgaccaaaag ttgccctatc gagccacggt
    21601 ccgccatttc cagcgtctga ctgttgaagt agaacgggaa gtgcgcccag gaaggctgac
    21661 cgaaataaac aagcagctcc acttgtccgt caacccaaac ggtatctttc cagcctctgt
    21721 cttccgggaa cggcatcgcg ccattcacgt tacggatctg gaacattacg ccttcaatat
    21781 ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc ccacgttcct tgctgcgcgg
    21841 tgacatcaat acggttgacg tcccacagct gtccattaat acccgggtca tcacccagac
    21901 tgatatcgcg actgcgaatt ggcgaaccag ccatgatttc agttggcagc aagcgcatcg
    21961 gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg cagcgttagc accagggtag
    22021 aaaccagaat actggatggc tcaaagaagc cgcgaatacg atcaacaatg ctcgccgctt
    22081 cgccacaggt gatcgacact tcatcgccgt tgctcatatc caccagaatc tcgcggcgct
    22141 cgcccggtgc cagcgaaagt tgcttcaccg atacaggagc aggcaggaat ccctgatcgc
    22201 cagaaatcac atgtaacggg cgaccatcgc tcatctgtag ttgatagcga cgagagttcg
    22261 acgcgttcag cagtcgcaag cgcacccagc cacgcgagac ttcaacgtac gggctttgta
    22321 caccgttaac cagcagcgta tcaccaacaa agccgccgct tcccggttcg ttgtattctg
    22381 gcgtaccaaa gttatccagc cgtttatcct ggataatgac cggaaaatca tccacaccat
    22441 aatggttggg gataggcagc gacttgctga cttcatcttc caccagccac attccggcaa
    22501 ggccgttata gacctgctga gccgtgcggt tgggagtatt ggcgtgatac cacagagtag
    22561 ctgcgttctg gcgaataggc agtacgggtg cccagtcagc gtttggcgac atcatccgtg
    22621 ccggaccgcc catcagcggg cctggtacct gtagcccggc caccgtcatt gagacatttt
    22681 ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc cttccagacg cggatagtcg
    22741 gccccaggta acgaccattg attccccaga ccgacgcgcg tgtccctggc gtaaatgacc
    22801 aatgcgcacg ttgtacagtc ataaacagcg gttgcccacg gcgagattca agtagcggcg
    22861 gaacgggtag cggttgctgt tgcccggctg cgctggcctt caggggaaca gcgcctgcac
    22921 aaagtgcaat ccccgatgcc tgaatgaact gacgccgact gagtgacata tttgctccat
    22981 gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc ccgcaaaaat acagattgcc
    23041 gacttaaact tttccggcgg cttcgcgttc tgcgacttct ttatcgagct cggcgatttt
    23101 ttgttccatt atcgaacgac aatgggcagc cagctcacga acctgatctt tgccatactg
    23161 actgacgtca attggcggca gcatttcgac aatcaccaga ccgttgtgca gtcgattaag
    23221 attaatctta ttcgaagttg tagagacgca cacgggaata atcgggacgc ccgccgcaat
    23281 tgccgcgtga aatgctccag tcttgaacgg tagcaggccg cgaccacggc tgcgggttcc
    23341 ttccgggaac atccagatgg aaatacggcg ttttttgaaa tgattcacga cttccgcaat
    23401 ggtgccgtga gctttagtgc gattgtttct gtcgatcaat aagttgccgg ttaaccagta
    23461 caactgcccg aagaagggga tccacagcaa gctcttttta cctaccgtca ccgtcggcgg
    23521 ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc tggtggttag cgatatagat
    23581 agcattgccg tagctttcag catctgcagg tttgcggcac tcaactttca ggccgaacag
    23641 cggcgcaaga cggccaaaca tatgcccaaa ggtggccaca tgtttcgggt tacgcgggct
    23701 gaaaaggcag taaatggagc cgaatacaca gactaagatg ctgtaaatca cggtaataat
    23761 aagacgaaag atatatagca taacaccctc tgaaggtctg agagactggc attgtacgtc
    23821 acctgaaatc aggttagggc attgttaaaa gccgctctct ggaaaagcgt attgttaatc
    23881 gcaacgcatg aataaacaac ggtttcaagg gaatttttat cgtttccggg ggttgaatac
    23941 gctgccggat gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga ggaaacaggc
    24001 cctccccggc atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct
    24061 caacacgatc gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag
    24121 tgactttctg taactcttcc gggcgcagtt taattttgcg tttcccaaca tgaatggtca
    24181 gcgtgctttg cggcggcaga acgtacaatt gcgccagacc atcttctcca cgcgcggctt
    24241 ctgccgatgg aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc
    24301 ttaccgggaa catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat
    24361 cttcaatcac caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac
    24421 ctgcacggtt acgcgccacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg
    24481 aagccatcag cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag
    24541 gcggcaacgt taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa
    24601 tcgggtcgat ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttgctct
    24661 tacctttcac cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga
    24721 tatcatggcc tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt
    24781 cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg
    24841 aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat
    24901 tcattttacg ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagttcac
    24961 tctgctcacc gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga
    25021 ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt
    25081 catcttcgtt acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac
    25141 cttccaggat atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca
    25201 cggtatcgcg acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg
    25261 gacgaccatc cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg
    25321 tagcgaagag gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca
    25381 ccagacgggt cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct
    25441 ttttgttgcg catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg
    25501 gtaatgcgct gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg
    25561 aaccacgtcc gttctcgtag attttacgga tctcggcgcg cgaagtgata atttccgctt
    25621 cagtcggata atccggcccc tgcacgatat ccagcagctg atcgagcgtg gttttcggct
    25681 ggtcgattaa tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg
    25741 tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgagcag
    25801 gcagcatttt cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct
    25861 gccccagctc gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca
    25921 ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg
    25981 gataacggta agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgctatcgc
    26041 cgtgcggatg gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg
    26101 cgctggcatt caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttaa
    26161 gaccatcgcc aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt
    26221 aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg
    26281 attcctcaac ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc
    26341 tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg
    26401 ataaaggcat caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag
    26461 ggcagcacaa ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt
    26521 cgagaagttg aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa
    26581 gtatacggtt cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa
    26641 gtgcggcttg ctcatggata cccccgagac ggcttacggc gcaccggata ataaatttcg
    26701 cgtatacatg gtattcctta ccggagaaat tataagccca acaacaatac gcctgtggca
    26761 ttgttcgctc aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca
    26821 cgccaacggc acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt
    26881 ggtgtagtga aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg
    26941 gcgaataatg ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac
    27001 ggcataacgt ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg
    27061 ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcagggtc
    27121 gtcccaggcg acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg
    27181 ccacatgata aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa
    27241 catcgccaca gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat
    27301 tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat
    27361 ttactttgcg gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac
    27421 cctgaatctc aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtggtcga
    27481 tatcaacatt gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt
    27541 caacgatatt gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta
    27601 cggtcgttac gctgccgttc ggcccctgga atcctccggc ctgactttgc gttgccgatg
    27661 ggccagaaaa accgccctgc tctgctgcca tcaccggtgc gctgcacagg gccattactg
    27721 cgattactgc tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat
    27781 ttaacaagat agtccttaac aacttcttaa gggaaaaaaa taaaatttag tgctgtacag
    27841 agcgcgttac aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc
    27901 gaattttact gatagaagat gacatgctga ttggcgacgg catcaaaacg ggccttagta
    27961 aaatgggttt tagcgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata
    28021 gcgcgcctta tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata
    28081 ttttgcgcga atggcgagaa aaaggtcagc gtgagccggt actgatcctg accgcgcgcg
    28141 atgcgctgga ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac
    28201 cttttgcgtt gatagaagtc gccgccaggc tggaagctct gatgcgccga accaacggcc
    28261 aggccagcaa cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca
    28321 cgctggctgg cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga
    28381 tgcgtaacgc tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg
    28441 acgaagaggt caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg
    28501 gtagtgattt tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt
    28561 tacccaacgt cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt
    28621 gacctggctg ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt
    28681 gttcgacacc caactgatgc tgtttgccaa gcggttaagt acgctcgatc tcaacgaaat
    28741 caacgcggcg gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga
    28801 cgatgcgctg acctttgcca tctttaccca cgacggcaga atggtcctta acgatggcga
    28861 taacggagaa gatattcctt atagctatca acgggaaggt tttgctgacg ggcaactggt
    28921 gggcgataaa gatcaatggc gttttgtctg gatgacctca cctgatggca aatatcgcat
    28981 cgttgttggt caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca
    29041 attgatcccg tggctggtcg cactgccgat tatgttaatc atcatgatgg tactattggg
    29101 tcgtgaactc gcgccgctga acaaactggc gctggcgctg cgtatgcgtg accctgactc
    29161 ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg cgtccgttgg ttgagtcgct
    29221 aaatcaactg ttcgcccgca cacatgcgat gatggttcgt gaacgacgct ttacctccga
    29281 cgcagctcac gaactacgta gcccgttaac ggcgctgaaa gtgcaaaccg aagttgcgca
    29341 gctttctgac gatgatccgc aagcgcggaa aaaagcgctg cttcaattac attacgggat
    29401 cgatcgcgct actcgtctgg ttgatcaact gctcactcta tcgcggctgg actcactgga
    29461 taaccttcag gacgtcgcgg agatcccgct tgaagatctc ctccaatcgt cggtgatgga
    29521 tatttaccac acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg
    29581 catcaaacgc accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa
    29641 cgccgtgcgc tacagtccac agggcagcgt gatagacgtc acgctgaatg ctgataattt
    29701 catcgtgagg gataacggcc ccggtgtgac gccagaggct ctggcgcgaa ttggcgaacg
    29761 cttctatcgc ccccccggac aaaccgctac cggcagtgga cttgggctat cgattgtcca
    29821 gcgaatcgcc aaattgcatg gcatgaatgt tgagtttggg aatgcggaac aaggtggatt
    29881 tgaggcgaag gtaagctggt aacttctctg tctatgagag ccgttaaaac gactctcata
    29941 gattttacta atagcaaaat ataaaccgtc cccaaaaaag ccaccaacca caaaccagac
    30001 aaataccgag cg
//
LOCUS       DAZFWP020000050        29660 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFWP020000050 DAZFWP020000000
VERSION     DAZFWP020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.050"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..201)
                     /locus_tag="ACHK0Z_RS10210"
                     /old_locus_tag="pgaptmp_002042"
     CDS             complement(<1..201)
                     /locus_tag="ACHK0Z_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU4502070.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGIL"
     gene            complement(204..923)
                     /gene="sanA"
                     /locus_tag="ACHK0Z_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(204..923)
                     /gene="sanA"
                     /locus_tag="ACHK0Z_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU4502071.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1073..1957)
                     /gene="cdd"
                     /locus_tag="ACHK0Z_RS10220"
                     /old_locus_tag="pgaptmp_002044"
     CDS             complement(1073..1957)
                     /gene="cdd"
                     /locus_tag="ACHK0Z_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU4502072.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEEALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2087..2782)
                     /gene="yohK"
                     /locus_tag="ACHK0Z_RS10225"
                     /old_locus_tag="pgaptmp_002045"
     CDS             complement(2087..2782)
                     /gene="yohK"
                     /locus_tag="ACHK0Z_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU4502073.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2779..3177)
                     /gene="yohJ"
                     /locus_tag="ACHK0Z_RS10230"
                     /old_locus_tag="pgaptmp_002046"
     CDS             complement(2779..3177)
                     /gene="yohJ"
                     /locus_tag="ACHK0Z_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU4502074.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3416..4366
                     /gene="dusC"
                     /locus_tag="ACHK0Z_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             3416..4366
                     /gene="dusC"
                     /locus_tag="ACHK0Z_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU4502075.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(4754..4837)
                     /gene="yohP"
                     /locus_tag="ACHK0Z_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             complement(4754..4837)
                     /gene="yohP"
                     /locus_tag="ACHK0Z_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU4502076.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            5061..6497
                     /gene="mdtQ"
                     /locus_tag="ACHK0Z_RS10245"
                     /old_locus_tag="pgaptmp_002049"
     CDS             5061..6497
                     /gene="mdtQ"
                     /locus_tag="ACHK0Z_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU4502077.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAGSNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            6550..7311
                     /gene="yohF"
                     /locus_tag="ACHK0Z_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             6550..7311
                     /gene="yohF"
                     /locus_tag="ACHK0Z_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4502078.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(7441..8019)
                     /gene="yohD"
                     /locus_tag="ACHK0Z_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             complement(7441..8019)
                     /gene="yohD"
                     /locus_tag="ACHK0Z_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4502079.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVAFGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            8189..8776
                     /gene="yohC"
                     /locus_tag="ACHK0Z_RS10260"
                     /old_locus_tag="pgaptmp_002052"
     CDS             8189..8776
                     /gene="yohC"
                     /locus_tag="ACHK0Z_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU4502080.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            8950..9882
                     /gene="pbpG"
                     /locus_tag="ACHK0Z_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             8950..9882
                     /gene="pbpG"
                     /locus_tag="ACHK0Z_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU4502081.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATAASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9920..11635)
                     /gene="dld"
                     /locus_tag="ACHK0Z_RS10270"
                     /old_locus_tag="pgaptmp_002054"
     CDS             complement(9920..11635)
                     /gene="dld"
                     /locus_tag="ACHK0Z_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4502082.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            11831..14128
                     /gene="bglX"
                     /locus_tag="ACHK0Z_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             11831..14128
                     /gene="bglX"
                     /locus_tag="ACHK0Z_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU4502083.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLINS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14339..15256
                     /gene="osmF"
                     /locus_tag="ACHK0Z_RS10280"
                     /old_locus_tag="pgaptmp_002056"
     CDS             14339..15256
                     /gene="osmF"
                     /locus_tag="ACHK0Z_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU4502084.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            15263..16420
                     /gene="yehY"
                     /locus_tag="ACHK0Z_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     CDS             15263..16420
                     /gene="yehY"
                     /locus_tag="ACHK0Z_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU4502085.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            16413..17339
                     /gene="yehX"
                     /locus_tag="ACHK0Z_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     CDS             16413..17339
                     /gene="yehX"
                     /locus_tag="ACHK0Z_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU4502086.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            17344..18075
                     /gene="yehW"
                     /locus_tag="ACHK0Z_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     CDS             17344..18075
                     /gene="yehW"
                     /locus_tag="ACHK0Z_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU4502087.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(18056..18163)
                     /gene="yohO"
                     /locus_tag="ACHK0Z_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     CDS             complement(18056..18163)
                     /gene="yohO"
                     /locus_tag="ACHK0Z_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU4502088.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(18223..18954)
                     /gene="mlrA"
                     /locus_tag="ACHK0Z_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     CDS             complement(18223..18954)
                     /gene="mlrA"
                     /locus_tag="ACHK0Z_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU4502089.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDILPLGI"
     gene            19176..20861
                     /gene="btsS"
                     /locus_tag="ACHK0Z_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     CDS             19176..20861
                     /gene="btsS"
                     /locus_tag="ACHK0Z_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU4502090.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            20858..21577
                     /gene="btsR"
                     /locus_tag="ACHK0Z_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /gene_synonym="yehT"
     CDS             20858..21577
                     /gene="btsR"
                     /locus_tag="ACHK0Z_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU4502091.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            21624..22094
                     /gene="yehS"
                     /locus_tag="ACHK0Z_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             21624..22094
                     /gene="yehS"
                     /locus_tag="ACHK0Z_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU4502092.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(22135..22596)
                     /gene="yehR"
                     /locus_tag="ACHK0Z_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /pseudo
     CDS             complement(22135..22596)
                     /gene="yehR"
                     /locus_tag="ACHK0Z_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
     gene            complement(22721..24721)
                     /gene="yehQ"
                     /locus_tag="ACHK0Z_RS10330"
                     /old_locus_tag="pgaptmp_002066"
     CDS             complement(22721..24721)
                     /gene="yehQ"
                     /locus_tag="ACHK0Z_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU4502093.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(24718..25854)
                     /gene="yehP"
                     /locus_tag="ACHK0Z_RS10335"
                     /old_locus_tag="pgaptmp_002067"
     CDS             complement(24718..25854)
                     /gene="yehP"
                     /locus_tag="ACHK0Z_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4502094.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(25847..28050)
                     /gene="yehM"
                     /locus_tag="ACHK0Z_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /pseudo
     CDS             complement(25847..28050)
                     /gene="yehM"
                     /locus_tag="ACHK0Z_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(28061..29149)
                     /gene="yehL"
                     /locus_tag="ACHK0Z_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             complement(28061..29149)
                     /gene="yehL"
                     /locus_tag="ACHK0Z_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU4502095.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYTLLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         7050 a         7610 c         8015 g         6985 t
ORIGIN      
        1 taaaattccg aagccgatat atgccaccat ataagcgaaa aagagtccgg gattttttgc
       61 tcgccagaca tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg
      121 ccagcctttc catgcttcgc ggaaacaaaa tatcgggata aggaaaaaaa cggcaacgac
      181 aaaaaactgc tgtacatcca taactacttt ccttgtttct tttgtaattc aagtaactgt
      241 tcgggtgtga cggcgggata cccctgcgca tcttccggca cctggtgcat agccggaata
      301 gggaccagcg gccctaaaaa acgcggttca cgtttaaaaa tataaaggtc agccagcgct
      361 ccgaaacggg cggcaaattc gcgaatacgt actgacagca tatctttcgg tgacggtacg
      421 gcataacact gagcctgaat ccccatatgc agcgcaataa ataatgcccg ctcacagtgg
      481 aaacgttggg tgataataat gaaatcatta gtatcgaaaa ctttgcgtgt acgcactatg
      541 gaatccagcg tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc gacaccagca
      601 gcgattaaat ctttgcgcat ggtcatcggc tcattataac tttgcaatgc gttatcgccg
      661 ctcagtaata gataatttac cttaccgctg ttataggcat taatcgctcc ttgaatgcgg
      721 tagcgataat actgattaat tacgccagta cgataatatt ttgctgttcc gagcaccaca
      781 ccgacctggc ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc ggttttccag
      841 ctcatccagc gatcgaggcc gagcacagtc aacagcagca agccgatcag gactaacagg
      901 ctgaggaaca cgcgctttaa catgcaggag actcgatcta agggaaattt ccatcaggct
      961 actttacccg ctggttttgc gcaagaaacg gcaaactgtt tgccgggatt ttcgacacca
     1021 ggagagggct ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag gcttaagcga
     1081 gaagcacgcg gtcgatactg tgacagccga gcgctttcag cgttgcggag gtggcatccc
     1141 actgaatcaa cggcgcatcg gctttttctg ccagaaccgc gcgctggata tccgggtaat
     1201 cataaccctt gagattcaac agaattaacg ctccctgcaa cggtggcaga gtcgggttga
     1261 atgcggcgtt ttcagcgtag ctgccactga aaatacgacc gtctttacat tccagcgcga
     1321 caccgcttgg cgacttactg taaggcatgt gcgaacggtt tgccgctgca atcgccgcct
     1381 gagaaagcgc atcaccagtc agcgcatagc cgtgatcctg ctcgtccatc agcagcgttt
     1441 taatctccag atctttcggc ccaaaggcat ccggcagata gtcacgcagc gcgtgtgcct
     1501 cgcggcccgg cagatgaata cgcagatcca gaccgctgtt cagttcattc ataaactgac
     1561 ggcagtgacc acaaggcgtg tagttaacgg tgatggctgc aagcgcttct tcaccactca
     1621 accaggcgtg gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt gtcgcaccaa
     1681 taaattccat attggcaccg aaataccagg ttccgctcac accgcgcgca attgcgccaa
     1741 cattaaaatt cgacaatggc gtacgcgcac aggccgccgc cagcggaagt agtgcgaatg
     1801 ccagcgcgtc ttcgtccagc cccgttgcgc tcttcagcga tgagacttgc tccccggtca
     1861 acaaagcggg gaagtacttg tctgccagaa taggttccag tgcagattgc aagttatccg
     1921 caagttgggc aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa
     1981 tgggataaca gtttacgaac ccgttatggc tttatatgtg atctgaatct cattaattat
     2041 gcaacgcgtg aaacttacat caaaaatgcg cgacgcatcg caaattttag cccattactg
     2101 ccagaataat cgggaaaagg aacggtgcga tcagcgaagt aattatcccg cataacacca
     2161 gcgctagcga actaaatgca ccttcctgat aatccagctc ggcgcagcgc gccgtaccga
     2221 gggcgtgcga ggcagtcccc attgccagac cgcgcgcagc tttggtacga atacgcatcg
     2281 cattaagcaa tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg
     2341 cgctgattgc cggaataccg ccaatactgc cgccaaccgc cattgcaata ggcgtggtga
     2401 ctgatttcgg caggattgac gcggcgattt ccggtgaagc gcccatcaac aatgccacgg
     2461 aagtccccgt caccatcgca accacgctgc cgataaaaca gatggtgatg atcgatttcc
     2521 agcgcgcgcg gatctggtgt agctgctcat ataaaggata ggccagcgcg acgaccgccg
     2581 gttgcagcag atcgtttagc acttcgctac ctttaaagta gctgtcgtaa gagatgccgg
     2641 tcagcattaa aaaaggaatg atcaccacca ttgctaccag caacgggtta agcaggggaa
     2701 acttataccg tgccgccagt ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg
     2761 accaccagat attcgccatc attcttctga tcctttctga cctacgactt tacgttcacc
     2821 gtgtaccagt tgtgaactcc agctcaccac cagaaaaacc accagcgtac tgacagcaca
     2881 ggaaaccact accgggccaa actgcgcgcg gagcaaatca aaatattgca tgacgcctac
     2941 gccaatcggc acaaacaata gcgccatata gcgaatcagt acgtagcacc ccggattgac
     3001 ccatttcgcc ggaagaattt gcaaggccag caggacaaac aggatcagca tcccgatgat
     3061 gctgcccgga atggttaccg gtagcaggga agcaatgaaa atgcctgcat acaggcaggc
     3121 ataaatcagg acgaaagcgc gtaaatattg ccagataatg ttcagtgtct tactcataat
     3181 caacacccca gatgagatgg attcatcata caattaattc gtaaaacgtg ctaccgatca
     3241 cattaagaaa tcagcgctta ttttttcgct ccccggcatt acgcaatgcg caacgaactc
     3301 aggcatacat caagcataga gagggattgc agtttgcagc aatccctctt tgtagacctt
     3361 taatccgctg accataggcg ctaacatagc gcctcattat tttgcgggtg atgatatgcg
     3421 tgtgttactg gcaccgatgg agggagtgct tgactctctg gtgcgtgaat tgctgaccga
     3481 agttaacgac tacgatctgt gcatcaccga gtttgtccgc gtggtggatc aactgctgcc
     3541 ggtaaaagtc tttcatcgca tttgccctga gctacaaaac gccagccgga caccatctgg
     3601 tacgctggtg cgcgtgcagt tgttaggtca gttcccacaa tggctggcag agaacgccgc
     3661 ccgtgcggtg gagttaggtt cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac
     3721 ggttaacggt agcggcggcg gcgcgacgtt actcaaagat cctgaactca tctaccaggg
     3781 tgcaaaagcg atgcgtgaag ctgtaccggc gcatttgccc gtcagcgtga aagtgcgtct
     3841 gggctgggac agcggtgaga agaaatttga aatcgccgat gcggttcaac aggctggcgc
     3901 tacggagctg gtggtgcatg ggcggacgaa agagcagggt taccgcgcgg agcatattga
     3961 ctggcaggcg attggcgata ttcgccagcg gctgaatatt ccggtgattg ccaacggtga
     4021 aatctgggac tggcagagcg cgcaacaatg catggcgatc agcggctgcg acgcagtgat
     4081 gattggtcgc ggggcgctca atattcccaa cctgagccgg gtagtaaaat ataacgaacc
     4141 gcgaatgccg tggccggagg tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca
     4201 gggcgatacc gggttatatc acgttgcgcg gattaaacag tggttgagtt atttgcgtaa
     4261 agaatacgat gaagcaacgg gattatttca gcatgttcgg gtgttgaata attcccctga
     4321 tattgcaagg gctattcagg caattgatat cgataagctg cgataatagt ggaatcgcag
     4381 cttatcagag acaatgaatc gcttattcag cttcatcatc ttgttgcgct cctttcatga
     4441 actaagccac atattgccac tggcgcaagg agcgcgcagg gggaggacaa tcgcctcctc
     4501 cccctgcacc cccgggctct ggcgaacaaa atcgccgctg cgcggtacct tcagcttatc
     4561 cccttcggct accgggtcgg gcgcgaggaa acgtccttgt aagacgcgcc ctcagcccac
     4621 atccatgtgg gctgccccgg ccttcgggga acgcttcagc gattttgacg ccaccaacac
     4681 cggagctgtt tgcaaatcaa gtacacaaaa tatctagttc ctgacctgac aacttatatg
     4741 ccatatctga tttttaaaat atcatcttaa atacgccagt caccaccagt agcccaatca
     4801 ggaaaataat caatacagcc cagagtataa ttttcattgg cgttccttta tttgtgagtg
     4861 ctgaataagt ataggaaaaa acgaagccct ttgccgttat ccataaacga ttaagataaa
     4921 tcggaagata tcattcactt agtgtatatt tttctggata tgtcgtttta ttgctactga
     4981 tgtattgcca cgcgaaacta aaaaatatat cgcaacacac ggtaatctcc gcgcaccttt
     5041 tcttacctga ttattccccc atgaatcgtg attcctttta tcccgccatc gcctgttttc
     5101 cgctgttact gatgctggcc gggtgtgcgc ctatgcatga aacccgccag gcgttaagcc
     5161 agcaaacgcc cgctgcacaa gttgacaccg cattacccac ggcgctgaaa aatggttggc
     5221 cagacagcca atggtggctg gagtatcacg ataatcaact cacttcctta attaacaatg
     5281 cactacagaa cgcaccggat atgcaggtgg ctgaacagcg cattcagcta gctgaagccc
     5341 aggcgaaggc aattgctact caggatggtc cgcagatcga cttttctgcg gatatggagc
     5401 ggcaaaaaat gtcggcagaa ggcttaatgg ggccgtttgc tctgaacgat ccggccgcag
     5461 gtacgaccgg tccgtggtac accaacggaa cttttggctt aacggcgggc tggcatctcg
     5521 atatctgggg aaagaatcgg gcggaggtta ctgcccgcct gggtacggtt aaagcacggg
     5581 cggcggaacg cgagcaaacc cgccaattgc tggctggcag cgtagcccgc ctgtactggg
     5641 agtggcaaac ccaggcggcg ttaaacacgg tcttgcagca aatagaaaaa gagcagaaca
     5701 ccattatcgc gaccgatcgc cagctatatc agaacgggat tacttcttca gttgacggtg
     5761 tggaaaccga tattaatgcc agcaaaaccc ggcagcagct caacgatgtc gcggggaaaa
     5821 tgaaaattat tgaggcacgg ttaagcgcac ttacaaataa ccagacaaag tcattgaagc
     5881 ttaaaccggt cgcgttgccg aaagtggcaa gccagcttcc tgatgaactg gggtactcct
     5941 tactggcccg gcgggcagat ttgcaggcgg cgcactggta cgttgagtca tcgctaagca
     6001 ccattgatgc ggcaaaagcg gcattttatc ctgacatcaa cctgatggcc ttcctgcaac
     6061 aggatgcgtt gcacttaagc gatctgttcc gtcattccgc gcagcaaatg ggcgttacgg
     6121 caggcctgac gctacccatt ttcgatagtg gtcgtcttaa cgccaatctc gatatcgcaa
     6181 aagccggaag caacttgtct atcgccagct acaacaaagc ggtggttgaa gcggtgaatg
     6241 acgtggcgcg ggcagccagt caggttcaga cacttgcgga gaaaaaccag catcaggcgc
     6301 aaattgagcg cgatgccttg cgtgtggtag gtcttgcgca ggcgcgcttt aacgcgggca
     6361 tcattgctgg ttcccgcgtc agcgaagcca gaatccccgc gctgcgtgag cgggccaatg
     6421 gcctgttatt gcaagggcag tggctggatg cttccattca actcactggt gcgttgggcg
     6481 ggggttacaa acgctgatga tatgcgcctt ctatacttaa cgtttattca gcgttaagtg
     6541 gagaactcga tggcacaggt tgcgattatt accgcctccg attcggggat cggcaaagag
     6601 tgcgcgttat tactggcgca gcaggggttt gatattggca ttacctggca ctcagatgaa
     6661 gaaggggcaa aagataccgc gcgtgaggta gttagccacg gcgtacgtgc ggagatcgtg
     6721 cagctggatc tcggtaaact gccagaaggt gcgcaggcgc tggagaaact cattcaacgg
     6781 ctggggcgta ttgatgtgct ggtgaataat gcgggcgcaa tgaccaaagc gccgtttctt
     6841 gatatggctt tcgatgagtg gcgcaagatt tttaccgttg atgtcgatgg tgcattttta
     6901 tgctcacaaa ttgcggcccg ccagatggtg aaacaagggc agggcggtcg catcatcaac
     6961 attacgtcgg tacatgaaca tacgccgctg ccggatgcca gcgcttacac agccgctaaa
     7021 catgcgctcg gcgggttaac caaaacgatg gcgctggagc tggtcaggca taagattttg
     7081 gtgaacgcag tcgcgcctgg ggcgatcgcc acgccaatga atggcatgga tgacagcgac
     7141 gtgaagcccg acgcggagcc ttcgattcct ctgcggcgtt ttggcgcaac gcatgagatt
     7201 gccagcctgg tagcgtggct gtgctcagag ggcgcaaatt acaccaccgg gcagtcgttg
     7261 atagtggatg gcggctttat gttggcgaat ccgcagttca acccagaata gcgcagtgag
     7321 gtgcgtggtt atttcaatgg gtaaaatttt ggtaggcatg ataagacgca caagcgtcgc
     7381 atcaggcatg gtgcaccgga tgtcggatgc ggcgcgaacg ccttacccag ggcaatggtt
     7441 ttacgcctga tgatccggct ttttcttccc gcgccgtttc agccaccagc gcacgcccac
     7501 caccagaacc acgaccagaa tcaaccagac ccagtgcttc aaatgctggt cgagattgtg
     7561 caaccacggc gcaatcacct gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca
     7621 ggcaaatgcg ccgagaatat tcagcggcag aaagattttc ggcggcagct ggctggcacc
     7681 aatcagcgtc gggccaatca cccgaaagcc atacataaag cgcgtaccaa tgacaaacag
     7741 atacggatgg cgttggataa gtttctgcgc ccgctcaatt ttatcctgat gtttcgagaa
     7801 acggcgtaac agcttgccgc caaaccgccg cccgcacaga taaagcacct ggtcgccaat
     7861 catgccgcca aacgccacag aaagtaccac cagcgggaac tttaatagcc cctgatgtgc
     7921 cgcaacgcct cccagcaaag tcacggtttc accttccgcc aggctaccga tcaccagcgc
     7981 ggcataacca tattgtgaga taagtgtatt gagatccata ggccagtctg tctccctttg
     8041 gttgccagta agcatacacc ctgtgaatca ttcacgctga aaaaggacgt tcatcagaca
     8101 gtgaaaatta catgggcgaa caaaaaataa tcacaagctg acttatactt gaactgtcag
     8161 gttcatgaac tcacaacaag ggggcggtat gagccatgtc tggggactgt tttcccatcc
     8221 cgatcgtgaa atgcaggtga ttaatcgcga aaacgaaacg atttctcacc attacaccca
     8281 ccacgttttg ctgatggcgg cgattccggt gatttgcgcc ttcattggca ctacacagat
     8341 tggctggaat tttggcgatg gcactatcct gaagttatcc tggtttaccg gactggcgct
     8401 ggctgtcttg ttttacggcg ttatgctggc tggggtcgcg gtgatggggc gggtcatctg
     8461 gtggatggcg cgcaattacc cgcagcgtcc gtcacttgcg cactgcatgg tcttcgcggg
     8521 ctacgttgca actccgctgt ttttaagcgg tctggtggcg ctttatccgc tggtctggtt
     8581 gtgtgcgctg gtcggcacgg tggcgctgtt ttacaccggc tacctgcttt accttggtat
     8641 tccgagcttc ctgaatatca acaaagagga aggccttagc ttctccagtt cgacgctggc
     8701 gattggcgtg ctggtactgg aagtgctgct cgccctaacc gttatactat ggggctacgg
     8761 gtatcgcctg ttctagaaag ttctttgaca ttgcattgct ggcgtaaatg tcagcaatgc
     8821 agcatttcct cacgattctc ctttgacgat cgcacttttt gctcgttatg atggcgtcgc
     8881 tagcctcagt aaatccttac tccggcggtg cgcaacccgt gcgcgtgaac cactatctga
     8941 atgctcatca tgccgaaatt tcgagtttct ttatttagcc tggccctgat gctggctgtg
     9001 cctttggcac cgcaggccgt tgcgaaaacg gcagccgcta ccgccgcttc acaaccggaa
     9061 attgcctccg gtagcgcgat gattgttgat ctgaatacca acaaagtgat ctattcgaac
     9121 cacccggatc tggtgcgtcc aattgcgtct atcagcaaat taatgaccgc gatggttgtg
     9181 ctggatgcac gactgccgct ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag
     9241 atgaaagggg tctattcgcg cgtacgactg aatagcgaaa tcagccgtaa agatatgctg
     9301 ttgctggcgc tgatgtcttc agaaaaccgc gcggcggcaa gccttgcgca ccattatccc
     9361 ggtggttaca aagcctttat taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac
     9421 aacacgcgct ttgttgaacc taccggattg tcggtgcata acgtttcaac tgcccgtgac
     9481 ttaaccaaac tgctcattgc cagcaaacaa tatccgttga tcgggcagtt aagtactacc
     9541 cgggaagata tggcaacctt ctctaacccg acgtatacgc tgccgttccg caatactaat
     9601 catctggtgt atcgcgataa ctggaatatt cagttaacca aaaccggctt taccaatgcg
     9661 gcgggccatt gtctggtgat gcgtacggtt atcaataata aaccggtggc gctggtagtg
     9721 atggacgcgt ttggcaaata tacccatttt gccgatgcca gccgcctgcg tacctggatt
     9781 gaaaccggta aagtgatgcc tgtgccggca gcagcgttga gctataaaaa acaaaaagcc
     9841 gcccaaatgg cggcggcggg gcagacggca cagaacgatt aagtgaattc ggatggcgat
     9901 actctgccat ccgtaatttt tactccactt cctgccagtt tttccgttta ctggttttac
     9961 cgatccccgg attcatgctg ttggtcggat cgttctcgcg atagaacttc tgcaacgtct
    10021 ccggtgcttt atacaaatga ccgacgttat gctcggcagg gtattgcgcg ccgcgctgct
    10081 gtagcagttc cagcatctgt tcttttaacg catgcacatc cacgcctttt ttcactatgt
    10141 aatcctgatg gaagacatag cacataaaat ggccgtaata gagcttgtgc accagctggc
    10201 tgtcgatctc cggcggtaaa tgctcatacc actcggtgtc gttacgccgc agagcgatat
    10261 ccaacgccag aatgtcttcg acttcatcgg aatgcaccgc ctgataacga attgctgcgc
    10321 ccgcagcggc gaaacggtgt aaaaacgctt tgctgccttc ctccggcgta cagacaaaga
    10381 aatcgccttc ggcctgtttg aaataatcca ccaaccacga tttggcttcg cccacgccat
    10441 cgcccgccat ttttaacagc agatgatgct cgtatttatc gcgccagttt ttcatgcgcg
    10501 gcggtaaatg gctggggaac aggtgaccga atttttgcat cgcacggtca gtaaaatgcg
    10561 gacggaagaa tttcactttc tccagcatcg catcggtgcg tcccttgaga ttaaagaaga
    10621 acggcatctt gtcggtgcct aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt
    10681 ccgcaatatc gtagatatcc cggtgcatat attccccggc aaccggcaga ttttcgaagt
    10741 tagccagaat atgacggcgg atttcggtca gcacttccgg ctggttggtg ccgatataaa
    10801 acacctgctg atttttttcc gcttcgaagg tatctagacg taccgcaaag acggccagct
    10861 tcccggcgca accagaagat tcaaataacc gatccggatc ggcgttataa cgtgcgggcg
    10921 tgtcggcttc aatatcacga acgcggtgga catagtcata atcgtgggcg tgacgaccat
    10981 cgtgacgcac atcgtcatct ttgatgcgat catcatccag cttgctaagg atctgctccg
    11041 gcgtttcgcc cagatcaatc cccagatggt tcaccagcgt cagtttgccg tcttcattta
    11101 tacgcgcgaa taacgacatt tcggtatacg ccgggccacg ttgcaccagc gagccgccgg
    11161 agttgttaca aataccgccg ataaccgatg cgcctataca cgacgatcca atcaccgagt
    11221 gcggttcgcg tcccagcggt ttgagggctt tttccagcga atagagcgtg gtgcccggat
    11281 aggccagcac ctgttcgccc ttgccaagaa cgtgcagctt gtcgagacgc agggtgctga
    11341 tgatcacgat atcgcgatca tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg
    11401 tattggcagc ctgcatcaga ataattttgt cggcggtgac acaggctttc agcacccgcc
    11461 acaattccag tagtgagcca gggaaaacga cagccagcgc gtcgccctga ccagaacgga
    11521 agcccttgcg atagcgggcc gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca
    11581 gacgggcaag ttcattcaaa aaggctttat tatcagttgt tgtcatggaa gacattttcc
    11641 actccttgtg gtggcgaaaa aatatcacaa actagaatag cgccttgtat gacaattcag
    11701 cgagtgaata ttttgaaaat tcagacaata aactgagcat tctttttctc tctccatcat
    11761 gcttatggca aactacggtt tttgcaccat tttcgctgcc tcgtcgggca acagaggaag
    11821 aaaaatccat atgaaatggc tatgttcagt aggaatcgcg gtgagtctgg ccctgcagcc
    11881 agcactggcg gatgatttat tcggcaacca tccattaacg cccgaagcgc gggatgcgtt
    11941 cgtcaccgaa ctgcttaaga aaatgacagt tgatgagaaa attggtcagc tgcgcttaat
    12001 cagcgtcggc ccggataacc cgaaagaggc gatccgcgag atgatcaaag acggtcaggt
    12061 tggggcgatt ttcaacaccg taacccgtca ggatatccgc gccatgcagg atcaggtgat
    12121 ggaattaagc cgcctgaaaa ttcctctttt ctttgcttac gacgtgctgc acggtcagcg
    12181 cacggtgttc ccgattagcc tcggtctggc ctcgtctttt aacctcgatg cagtgaaaac
    12241 ggtcggacgt gtctctgctt atgaagcggc agatgatggc ctgaatatga cctgggcacc
    12301 gatggtcgat gtctcgcgcg atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga
    12361 tacgtatctc acctcaacaa tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc
    12421 ggcagatcgc tactcggtga tgaccagcgt caaacacttt gccgcatacg gcgcggtaga
    12481 aggcggtaaa gagtacaaca ccgtcgatat gagtccgcag cgcctgttta atgattatat
    12541 gccgccgtac aaagcggggc tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc
    12601 gctgaacggc acgccagcca cctccgattc ctggctgctg aaagatgttc tgcgcgacca
    12661 gtggggcttt aaaggcatca ccgtttccga tcacggtgca atcaaagagc tgattaaaca
    12721 tggcacggcg gcagacccgg aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat
    12781 gagtatgagc gacgagtatt actcgaagta tctgcctggg ttgatcaact ccggcaaagt
    12841 gacgatggaa gagctggacg atgctgcccg ccatgtactg aacgttaaat atgatatggg
    12901 attgtttaac gacccataca gccatttggg gccgaaagag tctgacccgg tggataccaa
    12961 tgccgaaagc cgcctgcacc gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt
    13021 gctgaaaaac cgtctcgaaa cgttaccgct gaaaaaatcg gccaccattg cggtggttgg
    13081 gccactggcg gacagtaaac gtgacgtgat gggcagctgg tccgcagccg gtgttgccga
    13141 tcaatccgtg accgtactga ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct
    13201 gtatgccaaa ggggcgaacg ttaccagtga caaaggcatt atcgatttcc tgaatcagta
    13261 tgaagaagcg gtcaaagtcg atccgcgttc gccgcaagag atgattgatg aagcggtgca
    13321 gacggcgaaa caatctgatg tggtggtggc tgtagtcggt gaagcacagg ggatggcgca
    13381 cgaagcctcc agccggaccg atatcactat tccgcaaagc caacgtgact tgattgcggc
    13441 gctgaaagcc accggtaaac cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct
    13501 ggtgaaagaa gatcagcagg ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg
    13561 cggtaatgca attgccgatg tattgtttgg cgattacaac ccgtccggca agctgccaat
    13621 gtccttcccg cgttctgtcg ggcagatccc ggtgtactac agccatctga ataccggtcg
    13681 cccgtataat gccgacaagc cgaacaaata cacttcgcgt tattttgatg aagctaacgg
    13741 ggcgttgtat ccgttcggct atgggctgag ctacaccact ttcaccgtct ctgatgtgaa
    13801 actttctgcg ccgaccatga agcgtgacgg caaagtgact gccagcgtgc aggtgacgaa
    13861 caccggtaag cgcgaggggg ccacggtagt gcagatgtac ttgcaggatg tgacggcttc
    13921 catgagtcgc cctgtgaaac agctgaaagg ctttgagaaa atcaccctga aaccgggcga
    13981 aactcagact gtcagcttcc cgatcgatat tgaggcgctg aagttctgga atcaacagat
    14041 gaaatatgac gccgagcctg gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt
    14101 taagaaaggc gagtttgagt tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt
    14161 tgtaggccgg ataaggcgct cgcgccgcat ccggcaggaa acgccggacc gaatatgcct
    14221 cttaagtatt tatctgattc tgcaactgaa tccttccgct caagctaacc ccgccattat
    14281 caactatgct tttctcttaa ttcgctgaaa acaacagtaa aagaatgagg aaagcatcat
    14341 gccactctta aagctctggg caggttcact ggttatgttg gcagccgtga gcctgccgct
    14401 acaggcggct tcccccgtta aagtcggttc aaaaatcgat accgaaggtg cgctactcgg
    14461 caatatcatt ttgcaggtgc tggaaagcca cggcgtaccg acggtgaata aagtgcaact
    14521 cggaacgact cctgtggtgc gcggggcgat tacttccggt gaactggata tctatccgga
    14581 atataccggc aatggcgctt tcttctttaa agatgaaaac gatgcagcgt ggaaaaacgc
    14641 gcagcaaggt tacgagaaag tcaaaaagct cgattcggag cacaacaagt taatctggct
    14701 gacgcccgcg cctgcaaata acacctggac tatcgccgtg cgtcaggatg tggcagagaa
    14761 aaataaactc acttcgcttg ccgacctgag tcgttatctg caagagggcg gcaccttcaa
    14821 actggcagcc tcggcagagt ttatcgaacg cgccgatgcg ttacccgcgt ttgaaaaagc
    14881 ctatggcttt aagctcggtc aggatcagtt gctgtcactg gctggtggcg acacggcggt
    14941 gacgatcaaa gccgctgccc agcaaacctc tggcgttaat gctgcaatgg cttacggcac
    15001 tgacggcccg gtagcggcgc tggggctgca aaccttaagc gatccgcaag gcgtgcaacc
    15061 tatctatgcg cctgcaccag tggtgcgtga atcggtgttg aaggagtatc cgcaaatggc
    15121 acagtggcta cagccagtct tcgccagcct cgatgcaaaa acattgcagc aactgaatgc
    15181 cagcattgca gtggaaggac tggatgccaa aaaagtggct gccgactacc tgaaacaaaa
    15241 agggtgggcg aagtaatttc ccgtgactta tctacgtatt aatcctgttc tggcgctgct
    15301 gctgttgctg acggcaatcg cagcagcgct gccgtttatc agctacgcgc ctaatcgttt
    15361 agtctcgggt gaagggcgtc atctctggca actgtggccg caaacgatct ggatgctggt
    15421 gggcgttggt tgcgcctggc tgacagcctg ttttgtcccc gctaaaaaag gcagcatttg
    15481 tgcactcatt ctggcgcaat tcgtcttcgt attgttggtg tggggagctg gaaaggcggc
    15541 gacccaactg gcgcaaaatg gcagtgcgct ggcgcgtacc agcctcggca gtggtttctg
    15601 gctggctgcg gcgctggcat tgctggcctg tagcgatgcc atccgccgaa tctccacgca
    15661 tccgctgtgg cgctggttgt tgcatatgca gattgccatt attccgctgt ggttgctgta
    15721 ctccggcacg cttaacgatc tctcactaat gaaagaatac gccaaccgtc aggatgtgtt
    15781 tgacgacgcg ctggcacaac atctgacgtt gctgtttggt gcggtgctgc ctgcgttagt
    15841 gattggtgtg ccgttgggca tctggtgcta cttttccact gcgcggcagg gggcaatttt
    15901 ttctctgctc aatgtcattc agaccgtgcc ttcggtggcg ctctttggcc tgttgattgc
    15961 gccgcttgcc gcgctggtga cggcctttcc gtggctgggg aagctcggca tagcaggaac
    16021 cggaatgaca cccgcactga ttgcgctggt gctctatgcc ttgctgccgc tggtgcgcgg
    16081 cgtggtagtc ggtttgaacc agatcccgcg cgatgtgctg gagagcgcca gagcgatggg
    16141 catgagcggg gcgcagcgat tcctgcatgt tcagttacca ctggcgttac cggtatttct
    16201 gcgcagcctg cgggtggtga tggtgcaaac tgtaggtatg gcggtgattg cggcgttaat
    16261 cggcgcaggc ggttttggtg cgctggtttt ccaggggctg ctaagcagcg ccattgattt
    16321 agtgttgctg ggggtgatcc cggtaattgt tctggcggtg ctgatcgatg ccctgttcga
    16381 tttgcttatc gcactgctga aggtgaaacg taatgattga atttagccat gtcagcaaac
    16441 tgtttggcgc acaaaaagcc gttaacgatc ttaatctcaa ttttcaggaa gggagttttt
    16501 cggtgctgat tggcacatct ggctccggca aatccaccac cttgaaaatg attaaccgcc
    16561 tggtggaaca tgacagcgga gagatccgct ttgccggaga agaaattcgc tcgctgccag
    16621 tactggagtt gcgccgccgg atgggctatg ccattcaatc tattggcctg ttcccccact
    16681 ggagcgtggc gcaaaacatc gctaccgtgc cgcaattaca aaaatggtcg cgggcacgga
    16741 ttgacgatcg tatcgacgaa ttaatgacgc tactggggct ggagtcaaat ttgcgtgagc
    16801 gttatccgca tcagctttcc ggtggtcagc agcaacgtgt gggagtggcg cgcgcactgg
    16861 ctgccgatcc gcaagtctta ctgatggatg aaccttttgg cgcactggac ccggtaacgc
    16921 gcggcgcgtt gcaacaagag atgacgcgca ttcaccgttt gctggggcgc actattgtgc
    16981 tggtcactca tgatattgat gaggcgctac ggctggctga acatctggta ttgatggatc
    17041 acggtgaagt ggtgcagcag ggcaatccgc tgacgatgct gactcgtccg gcgaatgatt
    17101 ttgtccgcca gttttttgga cgtagcgaac tgggtgtgcg tctgctttcg ttacgtagtg
    17161 tggcggatta cgtgcgtcgc gaagaacggg cagaaggtga ggcactggta gaagagatga
    17221 cgctacgcga tgcgctctcc ctgtttgtcg cgcggggttg cgaggtgctg ccggtggtga
    17281 acacgcaggg ccagccttgc ggtacgctgc attttcagga tctgctggtg gaggcgtaag
    17341 cgtatgaaga tgttgcgcga tccgctgttc tggctcattg ctttgtttgt ggcactgatt
    17401 ttctggctgc cttacagcca gccgctgttt gctgccttgt tcccacaact gccacgaccc
    17461 gtttatcagc aagaaagttt tgcagctctg gcactggctc atttctggct ggtgggaatt
    17521 tcgagtttgt ttgcggtgat cattggcact ggtgccggaa ttgctgtcac tcgcccgtgg
    17581 ggcgcggaat ttcgcccact ggtggaaact attgccgccg ttggacagac ttttccgcct
    17641 gtcgcagtgc tggcgattgc cgttccggtg atcggctttg gtctgcaacc agcgattatc
    17701 gccttgatcc tttacggtgt gctgcccgtc ctgcaggcga cacttgccgg gctgggagcg
    17761 atcgatgcca gcgtgacaga agttgcgaaa ggtatgggaa tgagtcgtgg tcagcgactg
    17821 cgtaaggtcg agctaccgct ggcggctccg gtgattctgg cgggcgtgcg aacttcggtg
    17881 attatcaaca ttggtacggc gacgatcgcc tcaacggtag gggccagcac gctgggtacg
    17941 cccatcatca tcgggcttag cggatttaat accgcgtatg tgatccaggg ggcgttactg
    18001 gtggcactgg cggcgatcat cgcagaccgc ctgtttgaaa ggctggtgca ggcgcttagc
    18061 cagcacgcaa aataaaggta taacctgcga gcatgacgcc accaattccg ccgaacgcca
    18121 taaacaggaa cagggcgatg accccaattt tagctatgcg cataatgcac tccttatgtt
    18181 aacgaaagga ttgtacagta aagcgcattt gttaacgaat cattaaatgc cgagtggtaa
    18241 aatatcatgg ccttgttctt gccaactggt gagttgctgc tgttgggcgg aggttcgatt
    18301 ttcaccgcac cacaccagca atgtacggcc ttcgaatagt tcagggcgta gttgattgag
    18361 cgagtgggcg aggacatcaa tgcgccatcc ttgttgactg gcaatccagc cctccagcca
    18421 cagacgggtg gtatcctgaa tattccagcc aaccaccagc gcatctttac cctgtttttt
    18481 acgtgccgaa gccagacaaa tggcgatgta gttgatcagt acgccgtcga ggatcgccag
    18541 cagcgcctgg agagtcggtt gttggcattg aagtcgtcgg cgcagaggaa taaacagatg
    18601 tgtggtgagt gtctgggcgg ggtaatcctg accgcgctct ttgatccacg ttcgcaggct
    18661 atgcagattg ccgctttgca ggtaggtcag taatgtttct tgctgatcgc gccagccgtt
    18721 ctgcacatca acattttcat tactgagcag cattttaact ttgctgacct gcacgccgtt
    18781 gtcgatccag cgtttgatct cgcggatacg gtcaatatcg gcatcgttga acagtcgatg
    18841 accgccgtct gtccgttgcg gtttcagcaa cccgtaacgc ctctgccacg cgcgtaacgt
    18901 gacagggtta atatcacaaa gcaacgccac ttcaccaatt gtgtaaagcg ccatcgtctc
    18961 acccttgctc gcgaggtccc ggtttaactt tagacgcagt tttgcgaacc aggtagtttt
    19021 gcccgttttt tgtgcatcta tagggtgatt ttatttttgc caggcgattt tgagtgatcg
    19081 tactcacgaa ttctcatttt tctgcaagag ttcaaagaaa gttaaacgca ggcaatgtat
    19141 gttacgcgtt ttaaagggaa gtgtggtttg cgggtatgta cgattttaat ctggtgttgc
    19201 tgctgcttca gcagatgtgc gtttttttag tcattgcgtg gttaatgagt aaaacgccat
    19261 tattcatacc gttaatgcag gtcacggttc gtctgccgca taaatttctc tgctacatcg
    19321 tcttttccat cttctgcatc atgggcacct ggtttgggtt gcacattgac gattctattg
    19381 ccaatacccg tgcgataggc gcggtaatgg gcggcttact cggcggtccg gtcgtcggtg
    19441 ggctggttgg tctgaccggc ggcttacatc gatattcgat ggggggcatg accgcgctaa
    19501 gttgcatgat ctcaaccatc gttgaaggat tgctcggcgg cctggtacac agcatcctga
    19561 tccgtcgcgg gcgcactgat aaagtcttta accccattac cgccggtgcc gtcacgttcg
    19621 tcgctgaaat ggtgcaaatg ctgatcatcc ttgcgatcgc ccgaccttat gaagatgcgg
    19681 tgcgtctggt gagtaatatt gctgcaccaa tgatggtcac caataccgtc ggcgcggcgc
    19741 tgtttatgcg tatattgctc gataaacgcg cgatgtttga aaaatacact tctgcttttt
    19801 ctgccactgc gctgaaagtg gcagcctcga cggaaggcat tttgcgacag gggtttaacg
    19861 aagtgaacag catgaaagtg gctcaggtgc tgtatcagga gctggatatt ggtgcagtcg
    19921 cgattaccga tcgagagaaa ttgctggcct ttaccggaat tggtgacgac caccatttac
    19981 ccggcaaacc gatttcttcg acttacacct taaaagcgat tgaaaccggt gaagtggtct
    20041 acgctgatgg caacgaagta ccttatcgtt gctctttgca tccgcaatgc aaactggggt
    20101 cgacgctggt aattccgttg cgtggcgaaa atcagcgcgt gatgggcacc atcaaattgt
    20161 atgaagccaa aaaccgttta ttcagttcaa taaaccgcac gctgggcgag gggattgcgc
    20221 aactgctttc ggcgcagatt cttgccgggc aatatgagcg gcaaaaagcg atgctcaccc
    20281 agtcagaaat caaactgctt cacgcccagg tgaatcctca ttttttgttt aatgcgctta
    20341 acaccattaa agcggtgatc cgccgcgaca gcgaacaggc cagccagctg gtgcagtatc
    20401 tttccacttt tttccgcaaa aacttaaagc ggccttcgga gtttgttact ctcgccgacg
    20461 aaattgaaca tgtgaacgct tatctgcaaa ttgaaaaggc gcgcttccag tcgcggttgc
    20521 aggtcaacat tgctattccg caagaattat cccagcagca attgcccgcg tttaccctgc
    20581 aaccgatagt ggaaaacgcc attaaacatg ggacatcaca actgctggat acagggcgag
    20641 tggcaatcag cgcccgacgt gaggggcaac atttgatgct ggagatcgaa gacaatgccg
    20701 gtttgtatca accggtaacc aatgccagtg ggctggggat gaatctggtg gataagcgtt
    20761 tacgtgaacg gtttggcgat gactatggaa taagcgtcgc ctgtgagcct gatagttaca
    20821 cccgaataac gttacgacta ccatggaggg acgaggcatg attaaagtct taattgtcga
    20881 tgatgaaccg ttagcacggg agaacctgcg tgtatttttg caggagcaga gcgatattga
    20941 aatcgttgga gagtgttcaa acgccgtgga agggatcggc gcggtgcata aactgcgccc
    21001 ggatgtgctg tttctcgata tccagatgcc gcgcatcagt ggtctggaaa tggttgggat
    21061 gcttgacccg gaacatcgcc cgtatattgt ttttctcact gcgtttgacg aatacgctat
    21121 taaagccttt gaagaacatg cctttgatta tctgctgaag ccaattgatg aagcgcgact
    21181 ggagaaaacg ctggcgcgat tgcgtcagga gcgcagcaag caggatgttt cgctgttacc
    21241 ggaaaatcaa caggcgctga aatttatccc ttgtacgggg catagtcgga tttatttgct
    21301 gcaaatgaaa gatgtggcat ttgtcagcag tcggatgagc ggtgtctacg ttaccagcca
    21361 cgaagggaaa gagggcttta ccgaattgac attacgtacc ctggaaagtc gtacaccact
    21421 actgcgctgc catcgtcagt atctggttaa cctcgcgcat ttacaggaga ttcgtctgga
    21481 agataacggc caggccgagt tgattttgcg taatggctta accgtgccgg tcagccgccg
    21541 ttatctgaaa agcttaaaag aggcgattgg cctgtaaaag actgctaaaa tggctttttg
    21601 cctcatcaac acctgaaggc ctcatgctaa gtaacgatat tctgcgcagc gtgcgctaca
    21661 ttttgaaagc caataataat gacctggtgc gtattctggc gctgggtaat gtcgaagcca
    21721 ccgcggaaca gatcgccgtc tggctacgta aagaagacga agagggtttt cagcgttgtc
    21781 cggacattgt tttgtcgtca ttcctcaatg gcctgattta tgaaaaacgc ggcaaggatg
    21841 agtctgctcc ggcactggag ccggaacgtc gcattaataa caacatcgtg ctgaaaaaat
    21901 tacgcatcgc gttttcgctg aaaaccgatg acattctggc tatcctcacc gaacagcagt
    21961 tccgcgtttc gatgccggaa attacagcga tgatgcgcgc accggatcat aaaaacttcc
    22021 gcgaatgcgg cgatcaattt ttacgttatt ttctgcgtgg actggcagcg cgccagcatg
    22081 tgaagaaaag ctaagacggg tatggcggcc atgcgaaaca tggccgccga cagattattt
    22141 cacttcttta aaaccagcgg ctttcatcac cagttccatt tgcgccatag tgataccttt
    22201 tttggcatct tcagcagaaa cgttgattcc tgaaataccc tgcagggctt taaaatccac
    22261 tttttccata tcgatagtca cgttttcctg cgcgtaggta tctgtatagg ttaattattc
    22321 ttcaacaccc gcgatgtttt tgtatttggc gcttaacggc tcaagtgtct tggcagcgtc
    22381 ttctttggtg gttgcaccaa tagaagcaaa ttgaattttg gtttcagaag attgcttaag
    22441 caccttgtca cctttgtaga cataggtaat ggcaatttca gtgccgttca gattggcgct
    22501 gaatttcttc gattcttctt tgtcaccgca gccagcaaga gagaaaacca gaacagatgc
    22561 aacaacgagg gaaaacagct tattgaaagc cttcatgtaa aactccattt tatttaatca
    22621 agaaactggt gactctcacc aggggctata tagaatatgc ctaataccgt gacgtgagca
    22681 gtccggaact ggagtagaac tcttagtaaa aagcactatt tcatccttgt tgctgaagca
    22741 tggggaataa ttgttcgcaa agtaaaacac cgttattcat tgcttctacc cgtgcctcgc
    22801 tttctgtatt acgaaattgt cccaacacat gtgccagccg ataaaaaccg accgcggaga
    22861 ggtcattcgc cagcaactct gcctgactaa tagcgctctg ttcctgatag cgccagccgt
    22921 tatggagcag ttgaataagt aacgcctggc agcgcatcag caactgatga gcggtagacg
    22981 gcacaggcaa aacgctggca gaaggtagcg gtgccacagg cgcagtttct gcgtccagcg
    23041 cccaggcgcg ggtttttgtc atcatcaccc gtggttccag tgtcaattgc ccatcaacaa
    23101 aactgacaaa gccagaaacc agactcacgg ggtcgtctgt ttgttgcaaa agcgccgcca
    23161 tgcgttcaac ggcaaaagga gaacaggcag atgccggaag ggataacgtc aggagattat
    23221 cttcaccttc gccgctgatt acctgcgcat ccagcgtctg gcggctgcta tcccaaccga
    23281 gcgaaataca ctcagcgacc ggcagaataa ataagttatc gacctgatta agaggccgta
    23341 tgcaggcggg gggacgctgg cgtaaatatt cccgcaaagc cacaatgccc ggctggcgta
    23401 acggcgcgct caacatttgc caggcatcag gcgacagcgg cacaacgctg cttaagcggt
    23461 tgcgggtagc taacagcagc tcgccatcgg cactgcgttt tgctgcttgt gaaacaattt
    23521 gcccacccgc cagtgcgcca gcctgaaaac taaacagccg acgcgtagct gccggtgagt
    23581 tttcctgttc acttcgcggc caactgcgcg aaaggtgcaa aatactgccg gtgtcgggat
    23641 cggtaaacca gatgcgtaaa ccataatgct caatatcctg ccagcaacgc atacctaaag
    23701 acaccagccg cagatgatca agctttgctt ctccggcaat gccagagcca acgaccgtgc
    23761 gccacggcac aggaggaact tcaccaacac tgtcgcgccg ggccatctct tgtgcgcaat
    23821 ttaatcgact gtttaatgcc gcaagctgac gtaagcattc tccggcatga tagtggctgg
    23881 cgcgagtgtg gaaggcatca acgcttgccc gtagctgtcg tagtgattca ctcacccagc
    23941 gccagttgca ggcctctgcc gcctgcaatg tgcggttgaa tgctgcctcg tagtgaataa
    24001 gcggctggct gatgccgcca agccataatg cctggcttaa ttgctgaaca tattgacgac
    24061 acgtttggcc ttcttcgctg gcaaacggat cgtcagatga tgtgacgtgt tcgctgcgca
    24121 tctgccagat taaatggtta aattctgctt gctgcgcttt ggcctcgacg aaggcctgca
    24181 cagccagtac gacatgttcg caaagtgtgc cttcaataca atcacaacgg gcgaaacgaa
    24241 tactgctacg ggaataaaaa cgcacatcgc tcatcggtaa gcgggcagag ggaatttcgc
    24301 ccggcgcaca gaacaactca atggtgatgc ctttagcgac cagcgcctgc gcgcgtttgc
    24361 gggtggcatc gggcagggta gccagttctt ccagccagat tgccggatcc cactcttctt
    24421 ctttttccgt gggctggacg gtggcacaaa gtcgttgata acttaacacc agcatcacgc
    24481 gatgacggca catgccgctg gccccgcagc tgcactgagc ctctttcagt gcctggctgt
    24541 tcgccagctg ggtacggaca ccgtcactga aggtggcgat taaagcgccg ttctcatggc
    24601 tgatttccgg gacgttgcca ttttccagtt ccttaagact gcgcttaaca aaaccggcat
    24661 tgcttaacgc cgtcagtgcc tgcggtgtca gttctaataa ttccggacgt agtgaattca
    24721 tgactgaaga ttctccgcaa gccatgatgc cagctcgccc ggcgtcatgg cggctatttg
    24781 tgcgccgaca ttaaccagcg cctgggccgt atcgcggtca tagcaaggcg ttgcggtgct
    24841 atcgagcgct gccagtccca gcactttgat gccgctctgg acacactttt ttacctgatg
    24901 cgtcagcaat gatgatgaac ccccttcgta aaaatcgctc acgaggataa tgacgctttt
    24961 tgctggttgt tcaataagtt gccgaccata ctccacggca ctggcgatat tggtcccgcc
    25021 gcccaactgt actttcatta ataactctac cggatcggca acgtctgccg tgagatcaac
    25081 cacgcttgtg tcaaacgcca ccagatgggt acgaatgccg ggtaactgcc acaaacaggc
    25141 cgccatcacc gcagagtgga tcaccgagtc caccatcgat ccgctttgat caaccagtaa
    25201 gaccagttgc cattgttcgc tttggcgttt aatgcggctg ttaaagcggg gggattcgat
    25261 atacaacttg ccgtgttgcg ggtgccagtg ttgcaggttg gcgcgcagag tacttttgaa
    25321 atcaaagttt cgcgccagtg gaattaatga gcggcgacgg cgatcgcgga caccagaaaa
    25381 agcctgacga acttcctttg ccagtcgcgc cataatttct tcaacaacct ggcgcactat
    25441 ctggcgggcg gcagccagta cttcgggatt catcagatgt ttggtgtgca aaacggcgcg
    25501 tagcaggctt tccgaaggct gcatacgttc cagcacgtcg agattcgtca ccacatcttc
    25561 aatgccgtag cgcagtacgg catcgctttc cagtcgctca atcacctgtt gcggaaacag
    25621 agtgtgaata ctgttgatcc actcaggggt ggtgagattt gagccaccta atccaccaga
    25681 gcgttcacca cgctggagcc gttcaggatc gcgcccatac agccactcca gcgcgtggtc
    25741 tatctgccgg gcgttgtcat ccagcccaca aagcgtcgtt tctgccgctt cgccaagaat
    25801 taatcgccag cgttgtagct cacgggtggt cagaagatcg ttcagttcag acatggaaaa
    25861 ctccccagtt ttgcagcgat gccagtgcct gctgttcgag ttgttgatga tgtgcaatgg
    25921 cctgtggtgg acaatgcaac ggcatttgca gcgccgaaac gggaagttgc gccagttgat
    25981 aatgctcaag cacctgatgc gccagcgtcc cgcgttctcg tggtggtagc caggccatcg
    26041 ccgcgcgtaa atcgggcagg gcattgataa aatcggcatc acttagttga tttaaatgac
    26101 tgctgaaacc ggcgataaat gtcggctgac aggccagttg atggcgggcc agcgccagca
    26161 aaccgtgcag cgcttcgccg gattgtgccg gggataactg cgccagcatc gtcagcgccg
    26221 cttcggcact ggcgttagga tgttccagac gcatcagtgc gccatgagcc gcgccgcgat
    26281 ccagcgccgg ggcatgaatt gcctgactgc gtcgctccag taaggctacc gccgcagaaa
    26341 gcgaaacgcc ggataaatta acgccgctat gtagatcgcg cagaatatgg caaagcgctt
    26401 gccagctatg caggtgagcg tgaaactcct tttcatccgg tctgccgtta gattcacaca
    26461 gccacagcgt gcgatcgata gccgcgcata acgtcgtttg taatatctgc gcgccttgca
    26521 taccgctaat ttcatccagc cgccataagg catatagcac ttccagcgcc tggcccattt
    26581 cggcaaattg attttcctgg gcgattaata atgtgagttg ctccagtaat tgttgactga
    26641 aggacgccag acccgctaac gccgcctggc taagacatgt ggtgatactg ccgattccac
    26701 ccgcgtccag catatcggct tctaatttat ggcgtgcggc ttcctggagt gtggcaccaa
    26761 agcaggcggc ctcaatcaat gccgcatgct ggctaagcgg gcgggttaat ttccagtgtt
    26821 cttcaccgtt gcctgcaagt gtcagtgtgc ttccctgctg gcgcacaatc cctgggatct
    26881 ccagtattgc cagccgatgt aacacctgac tttgcgctag tccattgggg ttaaagcgat
    26941 ttaaggtaag ctccgccgga agggagattg cggtacgttc cagttcgcag gtgacatctt
    27001 ttggcagagg aggttgtggc gtagaagggg caagtttacc gaatccgtca cccgctaatg
    27061 tgtctatcaa cgttagcaga atcggatcgg tgtcgggatg aataacgccg cgatagctcc
    27121 acggcaacgg cgcattcagg gcttctttaa tcagcgatcc tgctatggca tccagccagt
    27181 cagtgcgtaa cggtagtgta tgaccgcgca actgtgccag cgccatcgca tgcagatgag
    27241 cggcagccat atccgcggtc gaagcaggca acttgtgctg gcgcaaacgg gtaaggatag
    27301 tttttagcag ttgttcaccg gcctgctgta agccccactg ccagcaccag ttttgccaga
    27361 ccggggcagg cataccggaa aggtatcctg ccagcacatc aaggcgcttt tcactgtagg
    27421 gcgtgagata acaacctgta atggcatctg ccagcgaggg caattccggc gtgttaatgt
    27481 cctgcgggca ttcgcgccac atttttgcca gtgccggagc gtgccagcca ccgcagacga
    27541 ctaacacgtc gccattattt tgctgcaccg cccatgcaat ccagcgagcc ataaaggctt
    27601 cgcgctgacg attgagtgca tcgccgggga aatcgccccg cagttgggca aaatagtgcg
    27661 ccagcgcaga gggtaatgca gtttgctggc tttcatcttg cgtatcaggc gaatcgtcct
    27721 cttcttcact ctgcgcccag caaggcaaat cgatgaagta agtttgcgcc tgaatacgac
    27781 gtgcggcttg tagcgcctgc cactccggcg aaaattcagc aaatggcgtc caggcaccac
    27841 gcccgggggc tgcaccgtcc tgatactggc aataactgta aatagctacc ggaagctggt
    27901 gggaaaaaaa agttcgtcta cccggtcatt aaaatcagcc gggccttcaa tcaacacgta
    27961 tcgtggccgc tggctttcga ttaacgattt caccagccgg gcgcaggccg gactatgatg
    28021 ccggatgccg acaattaacg gctcgctcat gcctctttcc tcacggcagg cggtggcggg
    28081 cttgataata agcctgccag tgagcttctt tatgtgtggc gacacgctgt tcgaagtaac
    28141 ggcgcagacg agcgcgatct tcttcgttat ctttgacgat ggttccggca atacagtcca
    28201 ccaggtcggc aggctccccc gcgcgattcg ctaaaaacca ggcgcggacg cctacggcgt
    28261 gggccacgtt cacggcttca gcggtggaca taatcgctgt cagtgtatcc attgacgttt
    28321 tcttttcgcc gttggcacgc agatcgcgga aggtgcgtac cagtaactcc agtactgcgt
    28381 caggcacttt atgcggaata ccgctatggg ccagcaaacg cgccgaagca ctggcgacca
    28441 gttccagctc ctgggcgaaa tccataatcg gaaagacggt ttcaaaatcg aagcgacgct
    28501 ttagcgcggc actcatctca ttgacgccgc gatcgcgggt attggcagtg gcaataatat
    28561 taaagccttc ccgcgcatag agctgactgg cttcaccggt gagttccggc accgtcatca
    28621 ccctgtcaga aagcatacct aacagacagt cttgtacttc cagcggtgta cgcgttatct
    28681 cttcaaaacg gacgattttg ccgtcgcgca ttccctggta aagcggggca gggaccagtg
    28741 cttccgttga cggaccatgg ttgataagca gggtgtagtt ccagccgtac ttgatttgat
    28801 cttcggtagt agatgcgccg ccctggatgg ttaacccggc atcgccgcta atagcggttg
    28861 ccagtaattc agacaggaga gattttgcgg tgccaggttc ccccaccaac atcaaaccgc
    28921 gcccggtggc aagagtgact aacatgcgtt cgacagcgga gggactaatc accactttac
    28981 ggctaatatt ttgcgcgcta tcgccaagga taaaagcacg ggccgcaggc aaacttaact
    29041 gccaaccggg cgggcaaggt gcgttatcat tttgttttaa ttttgccagt tcatcggcgt
    29101 ataacacagc agcaggcgga cgctgcagat gattattctg cggtgacata tattcatccc
    29161 tgaaatgtat taatgaaata aacgatattt tacgcgcaat aaatgtttgg cgcattagtt
    29221 gtttgagcga aaaatatctg gaaagcagat cacagtaaat aaaaaaacct gctggtgagg
    29281 caggttcttt tatttactac tttcaggcct aacaccacag cctgtgcaac atcgtgttcc
    29341 aacgaacgaa ttataaccac ggctggaccc cgttgcaatt aagataatga gatttataat
    29401 ccagaccgtt gggacgttgc gccggatcga atctatttca agtcctggct accggacggg
    29461 tggtgctgca ggtggtgccc tgatccggag tgcaacatcg tggtccaaca tacgtaaacc
    29521 agtatcgaca ggcagtgtaa gggtaaggtg ctggtgttgc gctccttaat aaggagagcg
    29581 gatggacacg ctgacacaaa agttaaccgt gctcattgct gtactggagc tattagtggc
    29641 tctgttacgg ttgattgatt
//
LOCUS       DAZFWP020000051        29625 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFWP020000051 DAZFWP020000000
VERSION     DAZFWP020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.051"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHK0Z_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHK0Z_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU4502096.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHK0Z_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             complement(804..1352)
                     /locus_tag="ACHK0Z_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU4502097.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHK0Z_RS10360"
                     /old_locus_tag="pgaptmp_002072"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHK0Z_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4502098.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHK0Z_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHK0Z_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU4502099.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKEQCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRAPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHK0Z_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHK0Z_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU4502100.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDENEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHK0Z_RS10375"
                     /old_locus_tag="pgaptmp_002075"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHK0Z_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU4502101.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHK0Z_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHK0Z_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU4502102.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHK0Z_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHK0Z_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU4502103.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHK0Z_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             complement(11782..12675)
                     /locus_tag="ACHK0Z_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU4502104.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHK0Z_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHK0Z_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU4502105.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAAPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHK0Z_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHK0Z_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU4502106.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHK0Z_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /pseudo
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHK0Z_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHK0Z_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHK0Z_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU4502107.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYSGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHK0Z_RS10415"
                     /old_locus_tag="pgaptmp_002083"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHK0Z_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU4502108.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFDHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHK0Z_RS10420"
                     /old_locus_tag="pgaptmp_002084"
     CDS             complement(17843..20107)
                     /locus_tag="ACHK0Z_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU4502109.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTIFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITTTDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAIHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGVRLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGRICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGMNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHK0Z_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHK0Z_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU4502110.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHK0Z_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHK0Z_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU4502111.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHK0Z_RS10435"
                     /old_locus_tag="pgaptmp_002087"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHK0Z_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU4502112.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHK0Z_RS10440"
                     /old_locus_tag="pgaptmp_002088"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHK0Z_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU4502113.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHK0Z_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHK0Z_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU4502114.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHK0Z_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHK0Z_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU4502115.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHK0Z_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             complement(26972..27664)
                     /locus_tag="ACHK0Z_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU4502116.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHK0Z_RS10460"
                     /old_locus_tag="pgaptmp_002092"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHK0Z_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU4502117.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7189 a         8052 c         7204 g         7180 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgta acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcgc gaacggcagg cgcaggttta
     2461 ctacgagatg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtccagca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgca gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgt tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccagccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgcgcg ctgccccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccagcg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttcaga cgtacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcgcggga ggttaactcc tcggtcagtc
     4441 ggctaagttc gatttccatc tgctcgatgg cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgtt
     4561 ccggacgttt tggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcgcgacgg tgtaagcgcc gttcaacgcc gttatctttc accatgcgca
     4741 tcactgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcagat tgtccatctc cgcttcgcag aaagtcagtg
     4861 ctttttccag ctggttgcgg cgggaccggt tattactcag ttgcgcatgc agctcgtcac
     4921 ggcgaattcg cgcccgctct tctgccccgc tatcggcgcg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 taaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatccccttc gatcaaatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttacctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acgccgccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacagcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcaccgca ctgctcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagt tcatcaatat caaaattctt gccctgacgt ttgcagaagt
     6481 ctgccagcag gcgttcggct tcttgctgct cgcgcagacg ctgttccagt tcacttaagc
     6541 gcatccgcaa cggttgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcagca
     6601 gttcgcgggc gacatcccaa gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcactgttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 cagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgca cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtagc tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgcgtcggca gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atagtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ctcttcgttt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc ggaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt ttcgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg actgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagccc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tgcgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacagc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc taaatctggc ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaaac gctgggcaaa gacgcggttt ttatccatat
     9241 cgatcgcagt acgaataaat ttgtgtacgt ggcgatcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccagctgata atacggtcga gtttgctctg cagatcgaac accagacgat
     9361 cgacaaaatg caggtcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaacttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtt ccggaagttt
     9481 ctgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 caatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatcag ctgcgcgttt gagttcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggaggtggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacaggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtattattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac gggcttggct gccaggtgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggtctt ttgcaaaacg
    13081 gtcacgatcc cacggaatgg tggcgcgtga gaagtacaga gcataccctt cagcgtcgag
    13141 aacgactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcgccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgatggtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 tgtttttctt ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tactctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc actatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgcca cggtgctcaa gtaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt cgtccagaat cagaatcggg ctatcacgca
    16321 acaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccata gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agttcgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gcaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgcgtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacgt tgcggaattc cacgtcacct gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagcgat ttcagcggac
    16921 gcataagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagcttgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcgaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc ctttagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atacaacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa
    17461 aaaaggaaac cggcattccc atcatgtgat caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgagac tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgttac ttgcccctga tgaggagtat cgagccacgt ataaccctgt tgttgatagc
    18001 gatgcttaac tttgttagag ggcagtcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gcctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt catcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtatgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcagg ccaggccagt cccgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacttg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagtaagc
    18661 ctccagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttccg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacgggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattctgc
    19321 caattaatgg tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt agggacggat aaccgtaccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcccgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttgacc atgaatggca cgaactttga gtttcattga ccacagttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca cgaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtgatttga ccgtaatgag tggtcatgcc gtcagtagta gtgatctcaa
    19861 caattgcatc ctgcgtcgca ccagttaagg tttctcctgc ccacaaaatt tgctttgccg
    19921 ctaatatacc ccaaacgaaa aacagcaacg tcagcgcgat ataacgtacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagatag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacgttca gaagaattta acgagagggt gaaaactgct
    20221 caaaactctg cgaagcagct tccagttcca gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct ctcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattactttg aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gcctggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccaggtg ggatgccagc
    23041 ggtaccagcg catcttccga tgcaacatca acatggtgat gtaatgccgc tagtgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccgccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt gatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgcccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acgaacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt atgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgcggt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 cgccacgctg atggctatct tcaaaaagga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggt gtctacgctt gccgcattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgcc
//
LOCUS       DAZFWP020000052        29466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFWP020000052 DAZFWP020000000
VERSION     DAZFWP020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.052"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(351..641)
                     /gene="nadS"
                     /locus_tag="ACHK0Z_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             complement(351..641)
                     /gene="nadS"
                     /locus_tag="ACHK0Z_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU4502118.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(642..953)
                     /locus_tag="ACHK0Z_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             complement(642..953)
                     /locus_tag="ACHK0Z_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4502119.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1182..2090
                     /locus_tag="ACHK0Z_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             1182..2090
                     /locus_tag="ACHK0Z_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4502120.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2154..3095)
                     /gene="fabY"
                     /locus_tag="ACHK0Z_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /gene_synonym="yiiD"
     CDS             complement(2154..3095)
                     /gene="fabY"
                     /locus_tag="ACHK0Z_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU4502121.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAIADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3140..3577)
                     /gene="dtd"
                     /locus_tag="ACHK0Z_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     CDS             complement(3140..3577)
                     /gene="dtd"
                     /locus_tag="ACHK0Z_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU4502122.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3574..4446)
                     /gene="yihY"
                     /locus_tag="ACHK0Z_RS10490"
                     /old_locus_tag="pgaptmp_002098"
     CDS             complement(3574..4446)
                     /gene="yihY"
                     /locus_tag="ACHK0Z_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU4502123.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4440..5039)
                     /gene="yihX"
                     /locus_tag="ACHK0Z_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     CDS             complement(4440..5039)
                     /gene="yihX"
                     /locus_tag="ACHK0Z_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU4502124.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5138..5923)
                     /gene="csqR"
                     /locus_tag="ACHK0Z_RS10500"
                     /old_locus_tag="pgaptmp_002100"
     CDS             complement(5138..5923)
                     /gene="csqR"
                     /locus_tag="ACHK0Z_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4502125.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5957..6853)
                     /gene="yihV"
                     /locus_tag="ACHK0Z_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             complement(5957..6853)
                     /gene="yihV"
                     /locus_tag="ACHK0Z_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGU4502126.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7021..7917
                     /gene="yihU"
                     /locus_tag="ACHK0Z_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             7021..7917
                     /gene="yihU"
                     /locus_tag="ACHK0Z_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU4502127.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7941..8819
                     /gene="yihT"
                     /locus_tag="ACHK0Z_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             7941..8819
                     /gene="yihT"
                     /locus_tag="ACHK0Z_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGU4502128.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            8836..10077
                     /gene="yihS"
                     /locus_tag="ACHK0Z_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             8836..10077
                     /gene="yihS"
                     /locus_tag="ACHK0Z_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGU4502129.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10215..11117
                     /gene="yihR"
                     /locus_tag="ACHK0Z_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             10215..11117
                     /gene="yihR"
                     /locus_tag="ACHK0Z_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGU4502130.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11316..13352
                     /gene="yihQ"
                     /locus_tag="ACHK0Z_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             11316..13352
                     /gene="yihQ"
                     /locus_tag="ACHK0Z_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGU4502131.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILIAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            13398..14783
                     /gene="yihP"
                     /locus_tag="ACHK0Z_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             13398..14783
                     /gene="yihP"
                     /locus_tag="ACHK0Z_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502132.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            14826..16229
                     /gene="yihO"
                     /locus_tag="ACHK0Z_RS10540"
                     /old_locus_tag="pgaptmp_002108"
     CDS             14826..16229
                     /gene="yihO"
                     /locus_tag="ACHK0Z_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502133.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            16297..16989
                     /gene="ompL"
                     /locus_tag="ACHK0Z_RS10545"
                     /old_locus_tag="pgaptmp_002109"
     CDS             16297..16989
                     /gene="ompL"
                     /locus_tag="ACHK0Z_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGU4502134.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(17080..18345)
                     /gene="yihN"
                     /locus_tag="ACHK0Z_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             complement(17080..18345)
                     /gene="yihN"
                     /locus_tag="ACHK0Z_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502135.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(18447..19427)
                     /gene="yihM"
                     /locus_tag="ACHK0Z_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             complement(18447..19427)
                     /gene="yihM"
                     /locus_tag="ACHK0Z_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU4502136.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(19435..20145)
                     /gene="yihL"
                     /locus_tag="ACHK0Z_RS10560"
                     /old_locus_tag="pgaptmp_002112"
     CDS             complement(19435..20145)
                     /gene="yihL"
                     /locus_tag="ACHK0Z_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4502137.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(20362..22185)
                     /gene="typA"
                     /locus_tag="ACHK0Z_RS10565"
                     /old_locus_tag="pgaptmp_002113"
     CDS             complement(20362..22185)
                     /gene="typA"
                     /locus_tag="ACHK0Z_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU4502138.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            22558..23967
                     /gene="glnA"
                     /locus_tag="ACHK0Z_RS10570"
                     /old_locus_tag="pgaptmp_002114"
     CDS             22558..23967
                     /gene="glnA"
                     /locus_tag="ACHK0Z_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU4502139.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            24141..25190
                     /gene="glnL"
                     /locus_tag="ACHK0Z_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             24141..25190
                     /gene="glnL"
                     /locus_tag="ACHK0Z_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU4502140.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            25202..26611
                     /gene="glnG"
                     /locus_tag="ACHK0Z_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             25202..26611
                     /gene="glnG"
                     /locus_tag="ACHK0Z_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU4502141.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            26723..26833
                     /gene="yshB"
                     /locus_tag="ACHK0Z_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             26723..26833
                     /gene="yshB"
                     /locus_tag="ACHK0Z_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU4502142.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(27023..28396)
                     /gene="hemN"
                     /locus_tag="ACHK0Z_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             complement(27023..28396)
                     /gene="hemN"
                     /locus_tag="ACHK0Z_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU4502143.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(28585..29094)
                     /gene="yihI"
                     /locus_tag="ACHK0Z_RS10595"
                     /old_locus_tag="pgaptmp_002119"
     CDS             complement(28585..29094)
                     /gene="yihI"
                     /locus_tag="ACHK0Z_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU4502144.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(29213..29379)
                     /locus_tag="ACHK0Z_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /pseudo
     CDS             complement(29213..29379)
                     /locus_tag="ACHK0Z_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7201 a         7630 c         7285 g         7350 t
ORIGIN      
        1 atggtgtagc cgaaaaacgt cagcatccta acagcaccag gatttaaggt gaaattaatc
       61 atccatcaca gaccggtcag tactgccacc aaccggtctt tacttgttca tcgttataat
      121 tttgttggta acagttcggg aaacaaggaa ttccttccgg aaccccccgg tatcatccga
      181 tagcattgac gtcaatcatc agcgcgacga tgagtttttt taatcgcatc atagggtggc
      241 gacgttcagc acgattacgg gtcatagcaa aactcctggg caggcgacca ctatgccgct
      301 ttcacaggag ttctgaatgc tagtgctttc tgtatggcat aaccatgcct ctaacggtta
      361 agtgcttgca gcacatgttg cggatcattg gcaatcgctc gcagcaaagc ctttgcaggc
      421 ccggtcggtg aacgtcgccc ctgctcccag ttacgcaacg tgtctacgct tacagaaata
      481 agatcggcaa atttagattg tgacaaccct gaagcgcggc gtatttcttt gattttcata
      541 gcatcaactt caaaagtccg cgaaggctgt cgcgttcctt caataatttc attcatctgg
      601 gccatgctgt gagtcaaccg ctcaaaaagt gccttatcca tttaccacct cgtatttaac
      661 aaccggagca ttgctttttc ctggggagac agatcatctt taataccctt acgataaata
      721 agcagtaatc tgatctcata atgtttaacc tgatgaaagt aaataaccct gacaccagca
      781 cgtttcccct ttcctcctga aacccatcga acctttctta acccaccagt ttcaggtatt
      841 acctcaccat agtcaggatt caaggccagg aaaaactgaa aacgactgaa ttcatcgtcg
      901 gtcagcagtt tttgaacatc ttcagtaaaa atttccgttt cgatgaagag cataattata
      961 cgccattggc ttagagtaac aatccttttt gtacgtcatt gacgtaccct tgaaaccact
     1021 agcttactga tagtgctcca gcaaaaccac gtctctgccg ggtttgtctt tagatttgcg
     1081 aggcgctttc cagatttttc acgcccgtga atttccctgt atccatccag caaaaacggg
     1141 tgtataagac tgtacattca tacagcaaaa taagttctac tatggcactg gaaaaaggaa
     1201 ttgcagagtt agtcgaaaag tttatcgctg cgggtcgccc ctcatcgcgt caacaaagta
     1261 ttgcgcagcg cagggagggc tatatcgcca gcgctgtcct tgcgggtgag acagaaacgc
     1321 gggtagatat tcagactatc gaacttgaag ggatgacctt acgaatagtg tcgccgctga
     1381 atgccccgac attactcccg acgataattt attactacgg tggctgtttt gttagcggcg
     1441 ggttcgcaac tcacgacaac cagctgcgcc agttagccta ttacgggcaa tgtcgggtta
     1501 ttgccgtcca gtatcggctg gcaccggaac atactttccc cgccgcccat gatgatgcgc
     1561 aacgaggtgc agaaatagtc tggcaacacg ccgaacgact cggcgttgat aaacaacgta
     1621 taactcttgc cggtgacagt gcgggcggac atctggcgct ggtaactgct ttgcgattaa
     1681 agcgtgctgg cgagtggcaa ccggcacaac tcatcttaat ttatcctatg cttgatgcga
     1741 ctgcgcattt cgaaagctac atacgcaatg gtcacgatta catcatcaca cgcgacactt
     1801 tactgagcgg ctttgagatg tatctgccag gcattgaacg caggcatcct gaagccagcc
     1861 ctctttggcg cgatgatttc aatggcttgc cacctgtaca tatcattacc gctgaatacg
     1921 acccactttg cgatgaaggt gaagcgttgt atcaccgcat gacagggcaa ggagtgcaat
     1981 gcacctgcca acgctacctt ggcgttatcc acggattttt tcagttaggt ggtatcagcg
     2041 aggccgcgcg atcggcacta cgggatgttg cctggagagc gggaaggtaa cttcggacct
     2101 gggccctgaa aaggttttca gattgaaaac cttttcagtc aagtaaaacg cccctactct
     2161 tcttcgttcc cgccctcttc atacgggcca aatggcttcg cgggcagaac gatatacgtg
     2221 ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt caacttgcat ctgcacccgt
     2281 gcttttcgtc cgcgcgccag acggtcgaga tcgccgctta aggcaccaag gtcggctatc
     2341 gcatgaggtt taccgctaat cggcttgctg tagcggatat gcgcatccgc cagaataatc
     2401 gttccgccga gatggcgttc gcgcagcatc agccagataa gcccccaacc ggtgagcgtc
     2461 gccagcgaga ataaactccc ggcaaacagc gtatggtgcg gattctgatt gccggtttcc
     2521 ggcatggtgg tgataaattt ttgcccggta tattgttgaa tgcgcacgcc cattttttcg
     2581 cttaatggaa tgtgttcata ccacgcctgt tgcagctggg cgcaccagtc accgcgatgc
     2641 agaatatcat ccagagtggc aacgggttta atcatcaaaa aatggcgaat cggcgtggta
     2701 gttggggtgg tgatctcccc ctgattaatg aatcccagct tggcgaaaaa ctccaccgcg
     2761 tcttcacggg cgctacaggt cacgcgctta acgccttcct gacgcgccac cgactccagg
     2821 gtcatcgcca tcagcgttcc taaccctttg tcctgcacgt cgggatgaac ggccataaag
     2881 cgaatggacg cttcattgtc ggcattaata tacaatcggc ctaccgccac cagattaccc
     2941 tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc acgcgtcgcg ttccgaacct
     3001 tttggttgat gcaggggctt acgcaacatt tcccagcgaa actgatagta acgctctaat
     3061 tcttcttctg tttgtggaac ccgaaggtga tacatagcga tactctctct tgtttcccgt
     3121 gacaaccctg gaagctggct catacctgca accagaatgt cacggggcca tcattgacca
     3181 gcgatacctg catatcggca gcgaagcgtc ctgtttgcgt gttcatctct tgctggcggc
     3241 agcgttcgac gaaatagtca tataacgcct ctgcgcgatc cggtgatgcg cctttggaga
     3301 aacttgggcg catccctcgt tcggtatctg cggcgagggt aaactgggaa accaccagta
     3361 cactgccgcc cgcctgttgc acgttgagat tcatcttgcc ttcggcatcg ctaaagatgc
     3421 ggtagccgag cacacgctcg cacagacggt ttgctttctg ttcgtcgtca tccttttcga
     3481 cacccaataa caccaaaagt cccgcgccaa tttcgcccgt cacttctccc tccacggtga
     3541 cgctggcacg ggttacgcgt tgaattaatg caatcatggt tcgtcgtctt cttcttgttc
     3601 agctgcttgt tttagtttgc ggtattcccc gagagtgaca gtaatttccg cgccaagcaa
     3661 gacgatacac catgtccagt agacccaaac aaagagaatg gggatcaccg ccagcacgcc
     3721 gtaaatgagc tgatatgacg ggaacatggt gatataaagc gcgaaacctt tctttcctgc
     3781 ttcgaacagg agtgcagcga caaacgcgcc gacaatcgcg tcgcggttag gtacgcggat
     3841 ggtaggaaca atgctgtaca gcaaccagaa ggagatccac gacaacagca gcggaaaaat
     3901 acgcagcacg ttgtcgatga cagtattgag atcgctcgcc cagcgcaggg agagcaaata
     3961 ggaactgatc gccagactgg cccctgccag cagcggcccc agcgttaaaa tcatccagta
     4021 cacggcaaac gagtaaattt ttggtcgcgc tcgtttactg cgccagatgg tattcaacgc
     4081 gctatcgatg gagtacatca acaataatgc ggtgacgatc agcccgcacg cgccaacggc
     4141 ggtcatcttg ttggaattgg caacaaattg ttcgatatac cgctgaataa catcgccagt
     4201 agcaggcaga aagttggcaa aaataaagtg acgcaactga atgctgacgt cggaaaacat
     4261 gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc ggcactaatg agagcaaaga
     4321 cacataggca aggttacctg ccagggttgt catgttgtcc tcatcaatac gttgccagag
     4381 tagttttagc caggcccata gtggacgagt acggtgcctg gctttgtcct gaatggtttt
     4441 tagcataaca ccttcgcgaa atagtccggg atggtggttt tatctttcac cagaatactg
     4501 gtaatgccca gctgattggc tccttctata ttatcggcgt tatcgtcgaa aaagaccgta
     4561 tcgctgggtg aaaaaccttc cgcctgcaaa acatgctggt aaattcgtgc ttcaggtttg
     4621 cgcatcccca gatcttgcga cagatagata tggtcagcag catcacgaat ttccgggtat
     4681 tcttccggcc agaaggtggt atgcaggcgg ttggtattgg aaagcaccac cacgcgatgc
     4741 ccctgctcac gcagtttatg catgatggcg atcacttccg ggcgcagcgc aacaaacacc
     4801 gcctgccagc cgtgagagaa ctgctcgtag cttagcggta gagccatctc atgacacagc
     4861 gcctctgcga acgcttcgtc gctaatttcc ccacgctcat gctgatgaaa cgcctccccc
     4921 atatgaaaac tcttcttaag cgatgccagc ggaatacgcg ttaaatcgct ccaggctccc
     4981 agcacacggt taaagtcgat atcgacaatc acattaccta aatcaaagat atagagcatt
     5041 tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg aaaggatggg tttggctatg
     5101 tggcaaggta tgaagccagg ttaacggtca caaaaatcta cgcgtcttcc tggggaagaa
     5161 tgatttcaat ttggctgtct tgtaagcgtg attttagcgc agcgggcggc agctcgtcgg
     5221 taaagagcgc agtgacctgt gccacgttac caatttcaac cgccgccgaa gcatgatact
     5281 tagtgtgatc ggcgaccagc agaatatttc tcgcgtgcgc catcatcgtt ttcaccacgt
     5341 tagcttcgtt tacatcaaac tccatcaacg cgccatcgct ctcaatcgcc ccaacgcttg
     5401 ttaccagata atcagcacga aaatcagcca caaaggacgc cgctgaaggg ccaatgatcc
     5461 cgctattatg agagcgcaac gtaccgccgg gcaccatcac ttcaaagcgc gggttgtggt
     5521 aaagaatatg cgccacacgc aggctgttgg tgattatccg caaatgatta tggttaagta
     5581 acgcccgggc aacatgctca acagtcgtac caatggtgat aaatattgtt gaaccatcag
     5641 gaatatagtc tgccaccgct tcggcaatcg cttttttttc ctcggtttgc gaaacctcac
     5701 gctgctcgaa cgccgtatta acgacgctgg aagcccgacc cgcgccaccg tgatggcgcg
     5761 taatcaggcc ttgctcgctt aatttacgaa tatcccggcg gaccgtctgc gtggagacat
     5821 ccagcagatt tgccagctca tcaatattca tatacccacg ctcggcgatc agcatgagga
     5881 gttggtcgtg ccgcgggtta ccggttagtt cggtaaggct catgaaaatt cctcgaaaaa
     5941 ccatcaccct ggcattttat acaaaaagtg acaaaaaaga tcgggtttga tcacagtcag
     6001 ggatcccggc tcgtccaccg ggacgtgtgc attttaacgc cgctacaccg ctggcgaagc
     6061 ggactgactc cgctaaatcc ccacttgttg ccagcgccac cgccaaagcg ccgtgaaaaa
     6121 catcacccgc acctgtggta tctaccacat caactttgaa ggccggttga tgctgacgcc
     6181 caccattttc cagccagtcg cagcctgcgc taccctgggt cacatagaca tgaccatttg
     6241 tgagcgtttg tgcctgtttt agcgcactgg ccatctcttt cacgcccgtt aagcgcgcca
     6301 gacccggttc tgaaaaggcc gcgtgatcgc ttaatgccac cagctcactg atatcctgcg
     6361 gcgtaatgtc cccgtccaga acggtcatca cacccgcctg acgggccagg gtgaaggctt
     6421 ttttagcgcc gtcgtgccag cgtacatctg ccagcacaac atcccactga gagaaatcaa
     6481 tttcctccaa ccactctgcg tcaggcagca gatccgggct ggggtagtta atgattatcc
     6541 gctcgccttt ggtatccacc atgatggcgg attgcgaaga tttcgcctgg ttataccgtt
     6601 tggtgtaacg ggtgttaacg ccccaggatt ccagctctgc cagcaggctg ttgcccgtgt
     6661 cgtcatcacc tacgcgacca ataaaatcga cctgcgcccc cagccttgcc gccgcaaccg
     6721 ctgccgtcgc cgctggcccg ccaccaactt ccgtataatt tctcgccacg tatttaccgc
     6781 tctccgtcgg taacccttcc acgtaataga tgcgatccat cacggttata cctacacaag
     6841 caacacgaat catggtcatt ccttaaacat tttgggatgc gttcatttta atttcaccaa
     6901 ttgtttaaaa agtgacggct gtcaattttt tgacgataaa ttacaaatac gatcaaaaac
     6961 agacaagcaa agcatgtcat aaatgacaaa aagtgacatc actgtattca ggagagggtt
     7021 atggcagcaa tcgcgtttat cggtttagga caaatgggtt cgccaatggc gagcaattta
     7081 ttgcagcaag ggcatcaact tcgcgtcttt gatgtgaacg ccgaagctgt gcggcatctg
     7141 gtagacaaag gtgcgactcc cgccgccaac ccggcgcagg ccgctaaaga tgccgaattt
     7201 atcattacca tgttgccgaa tggcgatctg gtgcgcaacg tgttgttcgg tgaaaacggc
     7261 gtttgcgaag gcttatctac cgatgcgctg gtcattgata tgtccaccat ccatccgctg
     7321 caaaccgata aattgattgc cgatatgcaa gccaaaggct tcagcatgat ggatgttccg
     7381 gtaggccgta cttctgcaaa tgccattacc ggtactctgt tactgctggc tggcggcacc
     7441 gctgaacaag ttgaacgtgc cacaccgatc ctgatggcga tgggcagtga gttgatcaac
     7501 tctggcggtc cgggcatggg gatccgcgtt aagctcatca acaactacat gagcatcgcg
     7561 ctcaatgcgc tttcggcaga agccgccgtt ttgtgcgaag ccctgaatct tcccttcgat
     7621 gttgccgtca aagtgatgag cggtaccgcc gccggtaaag gtcacttcac cacttcctgg
     7681 ccaaacaaag tcctcagcgg agatctttct cccgccttca tgatcgatct tgcccataag
     7741 gatcttggca tcgcccttga tgtcgccaac cagctgcatg tgccaatgcc gctgggggcc
     7801 gcctcacggg aggtttatag ccaggcgcgc gcagcgggtc gcggtcgcca ggactggtcc
     7861 gccattctgg aacaggtccg tgtcagtgcc gggatgactg ccaaagtaaa aatgtaacga
     7921 ctggataaag gaataaatga atgaataagt acaccatcaa cgacattacg cgcgcatcgg
     7981 gcggttttgc catgctggcg gtcgatcagc gcgaagccat gcgcatgatg tttgccgcgg
     8041 ctggggcacc cgctccggta gccgatagcg ttttaactga tttcaaagtt aacgctgcaa
     8101 aggccctctc gccttatgcc tcggcgattc tggtagatca acaattctgc taccgccagg
     8161 tggttgagca aaacgcgatt gccaaaagtt gcgccatgat tgtcgccgcc gatgagttca
     8221 ttcctggcaa cggtattccg gtcgatagcg tggttattga ccgcaaaatc aatccgctac
     8281 agatcaaaca agacggtggc aaagccttaa aactgctggt gctgtggcgt agtgatgaag
     8341 atgcgcagca acgtctggat atggtgaaag agttcaacga actgtgccac tcacacggtc
     8401 tggtaagcat cattgagcca gtcgtccgcc caccgcgtcg tggcgataaa ttcgatcgcg
     8461 aacaagcgat catcgatgcc gccaaagagc tgggcgacag tggcgctgac ctctacaaag
     8521 ttgaaatgcc gctttatggc aaaggtccgc aacaagagct tctctgtgct tcacaacgtc
     8581 tgaatgacca tatcaatatg ccatgggtga tcctctcttc cggtgtcgac gaaaaactgt
     8641 tcccgcgtgc cgtacgcgtg gcaatgacgg cgggcgcatc gggattcctg gcaggccgtg
     8701 cagtctgggc atcggtcgtc ggtttaccag acaacgagct gatgctgcgt gacgtttgcg
     8761 caccgaaatt acaacaactt ggcgatatcg tcgacgaaat gatggctaaa cgccgctaag
     8821 atgaggataa aaggaatgaa atggtttaac accctaagcc acaaccgttg gctggaacag
     8881 gaaaccgacc gcatctttga ttttggtaaa aattccgtag tgccgactgg ttttggctgg
     8941 ttaggcaata aagggcaaat caaagaagag atgggcaccc atctgtggat caccgctcgt
     9001 atgttgcacg tttattccgt tgctgcggcg atgggtcgac ctggcgctta ctcgttggtt
     9061 gatcacggta tcaaagccat gaacggcgca ctgcgtgata aaaaatatgg cggctggtat
     9121 gcctgcgtga atgacgaggg cgtggtggat gcctccaaac agggctatca acacttcttt
     9181 gctctgctgg gtgccgccag cgccgtcaca acgggtcacc cggaagcgcg caagctgctc
     9241 gattacacca ttgaaattat cgagaaatat ttctggagcg aagaagagca gatgtgcctg
     9301 gaatcctggg acgaagcctt cagcaaaacc gaagagtacc gcggcggcaa tgccaatatg
     9361 cacgcggtgg aagctttctt gattgtttat gacgtcactc acgacaaaaa atggctggat
     9421 cgcgcgattc gcgtggcttc cgtgattatc cacgacgtcg ccagaaataa tcattatcgc
     9481 gttaacgaac acttcgatac ccagtggaat ccgctgccgg attacaacaa agataacccg
     9541 gcgcatcgct tccgcgcgtt tggaggtaca ccaggccact ggatcgaatg gggccgttta
     9601 atgctgcaca tccatgctgc cctggaagcc cgttgcgaac aaccaccagc atggctgcta
     9661 gaagatgcca aaggtctgtt taacgccacc gtgcgcgatg cctgggcacc cgatggcgcg
     9721 gacgggattg tttataccgt tgactgggaa ggaaaaccgg tggtccgcga acgtgtacgt
     9781 tggcctatcg tcgaagcaat gggtacggcc tacgcgctct acaccgtcac tggcgatcgc
     9841 cagtatgaaa cctggtatca aacatggtgg gagtactgca ttaagtacct gatggactat
     9901 gaaaatggtt cctggtggca ggagctggat gcggacaata aggtcaccac caaagtctgg
     9961 gacggcaaac aggatattta tcacctgctg cattgcctgg tgatcccgcg tatcccgtta
    10021 gcgcccggca tggctccagc ggttgcagcg ggtctgctgg atattaatgc gaaataactg
    10081 atctgaaaat agggtgcggg ttttcccctc accctaaccc tctccccaaa ggggcgaggg
    10141 gaccgtccgg tgcagttttt gggtttgaca tcagcctcac gggaggctgt atgtcgttaa
    10201 ttaaggtgcc ttgtatgcaa atcaccaata tgcattgcag tggacagacc gtttcacttg
    10261 ccgcggggga ttatcacgcg accattgtga ccgtcggtgc tggtctggct gaattaacct
    10321 ttcagggttg tcatttagtc atcccgcaca aacccgaaga gatgccgctg gcgcatctgg
    10381 gtaaagtgct tattccgtgg ccaaaccgga tcgccaacgg ctgttaccgc tatcaggggc
    10441 aggaatatca gttacccatt aatgaacata gttcaaaagc cgctattcac gggctgcttg
    10501 cctggcgaga ctggcaaatt agtgaattaa ccgccaccag cgttaccctg acagcttttc
    10561 tgccgcccag ttacggctat ccgtttatgc tggcttcaca agttgtttat tcgctcaacg
    10621 cacacacggg tctttccgtt gagatcgcaa gccagaatat cggcactgtc gcagcgcctt
    10681 atggcgtggg tattcatact tatttaacct gcaacttaac atctgttgat gaatatctgt
    10741 tccagcttcc ggcaaaccag gtttatgccg ttgatgaaca cgtaaatccg acaacgcttc
    10801 accacgttga tgagctggat ttaaatttca cgcaggcgaa aacaatagcc gcgacaaaaa
    10861 tcgaccacac ctttaaaacc gctaatgatc tgtgggaaat aaccattacc catccacaac
    10921 aggcgctttc ggttagcctt tgttgcgatc aaccctgggt acaaatttat agcggtgaaa
    10981 aattacagcg tcagggactg gcagttgaac ctatgagttg cccgcctaac gcctttaatt
    11041 caggtatcga cttattatta ctggaaccag gaaaaccgca ccgcctgttt tttaacattt
    11101 acggacagag aaaataactt gtattgatta gcagtcaaaa tcattgaaga acatttcact
    11161 cgctaaaggc aacattatgt atcttcaatt tattaagtta aaaacagctc agcttctgta
    11221 aagtttttat atatcagttt tatgagaatc attttacata agcctgtaat aacaggaagg
    11281 caacaatctc gttctcaact ctatggagat tagttatgga tacgccacgt ccacagttat
    11341 tagattttca atttcatcag aataacgaca gttttaccct acattttcaa caacgtctta
    11401 ttttaaccca tagcaaagat aatccttgtt tatggattgg ctcaggtata gcggatatcg
    11461 atatgttccg cggtaatttc agcattaaag ataaactaca ggagaaaatt gcgcttaccg
    11521 acgccatcgt cagccagtca ccggatggtt ggttaattca tttcagccgt ggttctgaca
    11581 ttagcgccac gctgaatatc tctgccgacg atcagggccg tttattgctg gaactacaaa
    11641 acgacaacct taaccacaac cgtatctggc tgcgccttgc cgctcaacca gaggaccata
    11701 tctacggctg cggcgaacag ttttcctact tcgatctgcg tggcaaaccg ttcccgctat
    11761 ggaccagtga acaaggcgtt ggtcgcaaca aacaaaccta tgtcacctgg caggccgact
    11821 gcaaagaaaa tgcgggcggc gactattact ggactttctt cccacagcct acgtttgtca
    11881 gcacgcagaa gtattactgc catgttgata acagttgcta tatgaacttc gactttagtg
    11941 ccccggaata ccatgaactg gcgctgtggg aagacaaagc aacgctgcgt tttgaatgtg
    12001 ctgacacata catttccctg ctggaaaaat taaccgccct gctgggacgc cagccagaac
    12061 tgcccgactg gatttatgac ggagtaacgc tcggcattca gggcgggacg gaagtgtgcc
    12121 agaagaaact ggacaccatg cgtaacgcgg gcgtgaaggt caacggcatc tgggcgcagg
    12181 actggtccgg tattcgtatg acctcttttg gcaaacgcgt gatgtggaac tggaagtgga
    12241 acagcgaaaa ctacccgcaa ctggattcac gcattaagca gtggaatcag gagggcgtgc
    12301 agttcctggc ctatatcaac ccgtatgttg ccagcgataa agatctctgc gaagaagcgg
    12361 cacaacacgg ctatctggca aaagatgcct ctggcggtga ctatctggtg gagtttggcg
    12421 agttttacgg cggcgttgtc gatctcacta atccagaagc ctacgcctgg ttcaaggaag
    12481 tgatcaaaaa gaacatgatt gaactcggct gcggcggctg gatggctgac ttcggcgagt
    12541 atctgcccac cgacacttac ctgcataacg gcatcagcgc cgaaattatg cataacgcct
    12601 ggcccgcgct gtgggcgaaa tgtaactacg aagcccttga agaaacgggc aagctcggcg
    12661 agatcctttt ctttatgcgc gccggttcta ccggtagcca gaaatactcc accatgatgt
    12721 gggcgggcga ccagaacgtc gactggagtc tcgacgatgg cctggcgtcg gttgtcccgg
    12781 cggcgctgtc gctggcaatg accggacatg gcctgcacca cagcgacatt ggcggttaca
    12841 ccaccctgtt tgagatgaag cgcagcaaag agctgctgct gcgctggtgc gatttcagcg
    12901 ccttcacgcc gatgatgcgc acccacgaag gtaaccgtcc tggcgacaac tggcagtttg
    12961 acggcgacgc agaaactatc gcccattttg cccgtatgac ctccgtcttc accaccctga
    13021 aaccttacct gaaagaggct gtcgcgctga atgcgaagtc cggcctgccg gttatgcgcc
    13081 cgctgttcct gcattacgaa gacgatgcgc acacttacac cctgaaatat cagtacctgt
    13141 taggtcgcga cattctgatc gctccggtgc atgaagaagg ccgtagcgac tggacgctct
    13201 atctgccgga ggataactgg gtccacgcct ggacgggtga agcgttccgg ggcggggaag
    13261 ttaccgttaa tgcgcccatc ggcaagccgc cggtctttta tcgcgccgat agcgaatggg
    13321 cggcactgtt cgcgtcgtta aaaagcatct aatcacatcc gcccgtgggg aaactcatgg
    13381 gcaaagggag aacatcgatg agtcacatca caacggaaga tccagcaact ttacgcctgc
    13441 cctttaaaga gaaactctct tacggtattg gcgacctggc ctctaacatc ctgctggata
    13501 tcggtacact ttatcttttg aagttttata ccgacgttct ggggctgcca ggcacctatg
    13561 gcggcattat ctttttgatt tcaaaattct ttactgcgtt taccgatatg ggtaccggca
    13621 ttatgttgga ttcccgacgc aagatcggtc caaaaggtaa gttccgtcct tttattctgt
    13681 atgcgtcatt cccggtcacc ttactggcga tcgccaactt tgtcggcaca ccgtttgatg
    13741 tcaccggtaa aacggtgatg gccactattc tgtttatgct ttacggactg tttttcagca
    13801 tgatgaactg ctcctacggc gcaatggttc ccgctatcac caaaaacccc aacgaacgcg
    13861 cctcactggc ggcatggcgt cagggcggcg ctacgctggg cctgctgctg tgcacggtgg
    13921 gattcgtgcc agtgatgaat cttatcgagg gtaatcagca acttggctat atcttcgccg
    13981 ccacgctgtt ttcactgttc ggcctgctgt ttatgtggat ctgctactca ggcgtgaaag
    14041 agcgttatgt cgaaacccaa ccaaccaatc cggcgcaaaa gcctggcctg ttgcaatctt
    14101 tccgcgcaat tgccggtaac cgcccactgt tcattctgtg tattgccaac ctctgcacct
    14161 taggggcgtt taacgtcaag ctcgccattc aggtctatta cacccagtac gtacttaacg
    14221 atcccatcct gttgtcgtat atgggatttt tcagcatggg ctgtattttc atcggcgtgt
    14281 tcctgatgcc tggcgcagtc agacgttttg gtaagaagaa ggtctatatc ggcggcctgc
    14341 tgatttgggt gctgggcgat ctgctcaact atttcttcgg cggcggctcg gtcagctttg
    14401 tggcgttctc ctgcctggcg ttcttcggct cagcgtttgt taacagcctg aactgggcgc
    14461 tggtttccga caccgtcgag tacggcgagt ggcgcaccgg cgtgcgttcg gaaggcacgg
    14521 tctacaccgg cttcaccttc tttcgcaaag tgtctcaggc gctggctggt ttcttccccg
    14581 gctggatgct gacgcaaatt ggctatgtgc cgaacgtcgc acaggctgac cacaccattg
    14641 aagggttacg ccagttgatc ttcatctacc caagcgcact ggcggtagtc accattgtgg
    14701 cgatgggttg cttctacagc ctgaacgaga agatgtatgt ccgcattgtg gaagagatag
    14761 aagcccgtaa acgcacggcg taataataat aaacgacgcc ctgcggggcg ttataaggag
    14821 tgattatgtc tgaccataat ccactgacat taaaactgaa tctgcgggaa aaaatcgcct
    14881 atggtatggg cgacgtcggt tcgaatttaa tgctctgcat cggtactctg tatctcctca
    14941 aattttatac cgatgaatta gggatgcctg cttactatgg cgggattatc tttctggtgg
    15001 cgaagttttt taccgcgttt accgatatgc tcaccggatt tttactcgac tcgcggaaaa
    15061 atattgggcc taaaggcaaa tttcgtccct ttattttata tgcagcagtt ccggcggcgt
    15121 taattgcaac gctccagttt atcgccacca ccttttgttt gccggttaaa acgacgattg
    15181 ccaccgcact gtttatgatg tttggacttt catacagtct gatgaactgc tcgtatggtg
    15241 cgatgatccc ggcaattacc aaaaacccga atgagcgcgc gcagctcgcg gcttaccgcc
    15301 agggtggtgc aaccataggg ctattgattt gtaccgtggc gtttattccg ttgcagtcgc
    15361 ttttttctga ctcaaccgtc ggttatgcct gtgcggcact tatgttctcc attggcggct
    15421 ttatttttat gatgctgtgc tacagaggcg tcaaagagca ttatgtggac acagcgccaa
    15481 ccggacataa agccagtatt ctcaaatctt tttgcgcgat atttcgtaat ccaccattgc
    15541 tggttttatg cattgctaac ctgtgtactc tggcggcatt taatatcaaa ctggcgattc
    15601 aggtctatta cacccagtat gtgctgaatg atattaattt attgtcgtgg atgggattct
    15661 tcagtatggg atgcatcctc gtcggcgtat tactggtgcc agtaactgta aaatgttttg
    15721 gtaaaaaaca ggtttattta gctggcatgg tgctgtgggc ggtgggtgat atactgaatt
    15781 atttctgggg aagtaactct ttcactttcg tcatgttctc ttgtgtcgcc ttttttggca
    15841 cggcctttgt aaacagcctg aactgggcac tggtaccaga taccgtagat tacggtgaat
    15901 ggaaaaccgg tattcgtgcc gaaggttctg tttataccgg ttataccttc tttcgtaaaa
    15961 tttctgccgc acttgctggc ttcttgccag gcattatgct gacgcaaatt ggttatgttc
    16021 ccaacatcgc gcaaagcgat gcgacattac agggtttgcg tcagctcatt tttatctggc
    16081 cttgtgcact ggcaattatt gctgcattga cgatgggatt cttttacaca ctcaatgaaa
    16141 aacggtttgc attgattatt gaggaaatca accaacgcaa aaataaagag atggcgacag
    16201 aagaaaaaac ggcttccgta acgttataaa tgtcaatagc cgctatttcc attctggtgg
    16261 gtggcggcct ccctacgttt aaaaaatgga cttattatga aaaagattaa tgcaataatt
    16321 ttattatctt ctctgacctc tgcctcggta tttgctggcg catacgtcga aaacagggag
    16381 gcatataatc ttgcctccga tcagggcgag gttatgctac gtgtgggtta taacttcgat
    16441 atgggcgcgg gtattatgtt aaccaatacc tacacctttc agcgagaaga tgaactaaaa
    16501 catggctata atgaaattga aggttggtat cctttattca aaccaaccga taaattaacc
    16561 atccagcccg gtggcttaat taatgataag agtatcggtt cgggtggtgc agtgtatctg
    16621 gacgtcaact ataaatttgt accatggttt aatctgacag tacgaaatcg ctataaccat
    16681 aataactata gttcaacaga tttgagcggg gaactggata ataatgacac ctatgaaatt
    16741 ggcacctact ggaattttaa aatcaccgat aaattttcct atacatttga gccacattat
    16801 ttcatacgcg taaatgactt taatagcagc aatggaaaag atcatcactg ggaaatcact
    16861 aataccttcc gttatcgcat taatgaacac tggctgcctt atttcgaatt acgttggtta
    16921 gatcgtaatg tcgaaccgta ccaccgcgag caaaaccaga ttcgtatcgg gacgaagtat
    16981 ttcttctgat gtgaaactgc cggatgcggc gtaaacgcct tatccggcct acggttgata
    17041 cggttttcgt tggccggatt aagcacatcc ggccattaat caagcttcag ccgccagcat
    17101 ctctttctta cgttggctaa ttcgcttaac cagcattacg gaaaccaccg caccacagaa
    17161 tgcgaagcag gccataatgc caaacacgat tttgtagcca ataatccccg gatttaaatc
    17221 cagaatgtag ccatacagac tgaagcagaa catcgccgga gcgtaaccaa taaagctacc
    17281 caacgccatc gccgcgcctg ttttattttc agcaattttt gcttcgccga taggtgcaaa
    17341 aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg ccatccctaa
    17401 atagaccggc atactttcgt gcggcagcat aatcaacagt acgagcgcag cggtactgat
    17461 gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg aaatcatgcc
    17521 accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt aagcccccac
    17581 cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg tcaggccaca
    17641 gtaaaccgcg taaacgaaga agacgttaaa agcaataagc cagatagttt tgtctttcag
    17701 caccgaggtc atactggtgt ttttcgatgc tccgtcttct tttttcacct cgacggacgg
    17761 tgcctcttct ttgtcattca gaacaaagaa aatgataatg cctacggcaa tcacaataag
    17821 ggaatagaac cagatgcctg ctttaaaacc caataagcca ctgccaaacc aggtaaatac
    17881 tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt cgaagaagcc
    17941 aaacaaccga ccttgttgtt cgctattgcc caatcgactc accgacttga gcaagaccgg
    18001 ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga tgccccaata
    18061 ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg agaatgacat
    18121 ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac agaaaaaaac ccactgtcgt
    18181 gacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat ggaaatattc
    18241 ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg taccgccaca
    18301 caatgttaat agactaaata acgcccattt ctttttcgtg agcataacat cccctaagcg
    18361 atttttaaaa tcaatgtttt aaaggcaata agataccgac cattcatatg gaatggtttt
    18421 attaactgcg tatgagttaa aacttattag tggttcagaa gatggtttgc ctcttgtttg
    18481 atttgttgta gtacgttacg cacatgattt atctgattaa ttgcatcttc tttttcttta
    18541 cgtaaccgcg catccagtaa atgattttct ggcaatggtg tttcactcat ctggcgataa
    18601 gggatccacg gattatcatc ttcgtcttca aagcggaacg ccggcgcata gtaatcaacc
    18661 tcttcttcca ggccatatgc cgtcacctgc ggctcatcat cacccaggca tataaggtgc
    18721 gttaataacg ctttgaacgg catatcaccc tgacctgaaa tacaggcttt atgacccagg
    18781 ccacccggtt ctttaacgat caaggcatct ttgatatgga cctgggtaat atgcggcgcc
    18841 atcgttttta aagcgtcgat gggatgctca tttgcattga tcatgttcgc aaaatcaaac
    18901 agtaaggaaa gagattccat ctcactttct ttgaccagcg acaccagctc atggcttttt
    18961 aaatcttcat gctgttcgat cgtaaaagtc aggccgctgt cctgatacgt ttcccgtaca
    19021 taggcaatgt cgttagcgat gatcgataat acgtcgcgca aattaccttc ataacgtgga
    19081 taaaaacgta cggacgatgc cccagttttc aacgcaatgg cgacggcttc gtcaatagat
    19141 gccttatctg aagcgctggt ttcaatatga atatcaaggt tcagacggcg ggctttatcc
    19201 ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc gccatccaga
    19261 acatggattt tgacgccacg tagattattt tcacttgcga tatcaagtaa atcagcaggc
    19321 aacactcttt ccagccgcat atttaaatga aaggcataag catgtaaata aagaggaaga
    19381 gtgtcgacac gttgtagaat ctttcttgcg ttatttattg tcaccataaa acacctacag
    19441 attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct ggacaatatt
    19501 gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg cagaaccgcc
    19561 aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt tttttactac
    19621 cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt caagttgttc
    19681 aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa ctttatgtcc
    19741 ttccagcgcc ccccagccgg tgatgcgata gagttcagtt gatggtgtca ctgcaatcag
    19801 tggcgcgagc gtctcgggaa tatcgctggt acggtttttc tcggtaaacc cccaggaagg
    19861 ttcccgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg ggctgtaatt
    19921 aaatcgcggc tgagtcacga accacccttt acgatcctta cggaagattt tggattctgc
    19981 ctcgaggttc agcaacgcct ggcgcagcgt catacgttta atgcccagca gctctcccag
    20041 ctctctttct gaagggagtt ttccacccgg cgtagtgatg ccatctttta accaccgatc
    20101 cagtttctct tttgcatttt ctacggtgga ttgatttccc gtcattgttt aatccttttg
    20161 gtttaaacca gatgaggcat cttacgctta cgccaaaaaa gaaagcaatt aacggcgtat
    20221 gactttgcag ataattgtga caaagctctc tattgacgta accacagact tataagggag
    20281 atactcgcat tgctgagtgc tcacgacagg caaaaaaaaa cctgccatcg ctggcaggtt
    20341 ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg cggttggcgc gacgacgatc
    20401 gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggggtcactt ctaccagttc
    20461 gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg gaaccagaac
    20521 aacggcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg tcaggcagtt
    20581 tacagtcagg tcgttagagc ggctatgaat accgataatc tgaccttcgt aaacttctgc
    20641 accgtgaccg aggaacagct taccgcgatc ctgcagaccg aacagcgcga acgcgaccgc
    20701 tttaccctga ccgttagaga tcagtacgcc gttctgacgc tgacccactt cacccggacg
    20761 tacgtcgtcg tagtggctga aggtggagta cagcagacca gtaccggaag tcatggtcat
    20821 gaactcagaa cggaagccaa tcagaccacg gcttgggatc acgtagtcga gacgtacgcg
    20881 gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca gcgcctgcat
    20941 tacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg ttttcatacg gctcttgttt
    21001 acgaccgtcg atttcacgga agataacttt cggacgggat accgccagtt cgaaaccttc
    21061 acgacgcatg ttttcgatca gaacagacag gtgcagttcg ccacgaccag aaacgcggaa
    21121 cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg ttatgtacca gttctttgtt
    21181 cagacgatcc aggatctgac gagacgttac gaacttacct tctttaccgc agaacggcga
    21241 ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg ccggcagcgc
    21301 ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccaa ggcccgtgat
    21361 cgccacgata tcgccagctt ccgccagatc ggtttcgata cgttccagac cgaggtggcc
    21421 cagcacttta ccgactttcg cgttgcgggt tttgccttcg ctatcgatga tagtgacctg
    21481 ttggttcggc ttcactttac cgcgcttgat gcggccaatg ccgataacgc caacatagct
    21541 gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa cgtccggcgc
    21601 aggaacgtga tcaacaatcg cctgatacag cggggtcatg tcttccgcca tatcttcgtg
    21661 gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt ccagctgctc
    21721 gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc aatcaggacg
    21781 cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt aagcaaacgc
    21841 ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa ccaccagcag
    21901 cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg cgtgccccgg
    21961 ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt tcgcgaggat
    22021 ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt gggtttcggc
    22081 acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg ttttaccatg
    22141 gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt tgcctcaggc
    22201 attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg atcacaaaca
    22261 tcctccgcaa acaagtattg cagagtccct ttgtgatcgc tttcacggag cataaaaagg
    22321 gttatccaaa ggtcattgca ccaacatggt gcttaatgtt tccattgaag cactatattg
    22381 gtgcaacatt cacatcgtgg tgcagccctt ttgcacgatg gtgcgcatga taacgccttt
    22441 taggggcaat ttaaaagttg gcacagattt cgctttatct tttttacggc gacacggcca
    22501 aaataattgc agatttcgtt accacgacga ccatgaccaa tccaggagag ttaaagtatg
    22561 tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt tgatttgcgc
    22621 ttcaccgata ctaaaggtaa agaacagcac gtcactatcc ctgctcatca ggtgaatgct
    22681 gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg gaaaggcatt
    22741 aacgagtccg acatggtgct gatgccagac gcatccaccg cagtgattga cccgttcttc
    22801 gccgactcca ccctgattat ccgttgcgac atccttgaac ctggcaccct gcaaggctat
    22861 gaccgtgacc cgcgctccat tgcgaagcgc gccgaagatt acctgcgttc cactggcatt
    22921 gccgacaccg tactgttcgg gccagaacct gaattcttcc tgttcgatga catccgtttc
    22981 ggatcatcta tctccggttc ccacgttgct atcgacgata tcgaaggcgc atggaactcc
    23041 tccacccaat acgaaggtgg taacaaaggt caccgtccgg cagtgaaagg cggttacttc
    23101 ccggttccac cggtagactc ggctcaggat attcgttctg aaatgtgtct ggtgatggaa
    23161 cagatgggtc tggtggttga agcccatcac cacgaagtag cgactgctgg tcagaacgaa
    23221 gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat ctacaaatat
    23281 gttgtgcaca acgtagcgca ccgcttcggt aaaaccgcga cctttatgcc aaaaccgatg
    23341 ttcggtgata acggctccgg tatgcactgc cacatgtctc tgtctaaaaa cggcgttaac
    23401 ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg cgctgtacta cattggcggc
    23461 gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc cgaccaccaa ctcttataag
    23521 cgtctggtcc cgggctatga agcaccggta atgctggctt actctgcgcg taaccgttct
    23581 gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga agtacgtttc
    23641 ccggatccgg cagctaaccc gtacctgtgc tttgctgccc tgctgatggc cggtcttgat
    23701 ggtatcaaga acaagatcca tccgggcgaa gccatggaca aaaacctgta tgacctgccg
    23761 ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc actgaacgaa
    23821 ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt tcactgacga agcaattgat
    23881 gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc gcatccggta
    23941 gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc gcaacaacgc
    24001 cgggatttag ttgccgtgga aactttcagc ccatctctgc atgggctttt ttctccgtca
    24061 attctctgat gcttcgcgct ttttatccgt aaaaagctat aatgcactaa aatggtgcaa
    24121 cctgttcagg agactgcttt atggcaacag gcacgcagcc cgatgctggg cagatcctca
    24181 actcgctgat taacagtatt ttgttaatcg atgacaacct ggcgatccat tacgccaacc
    24241 ctgccgcgca acaactgctc gcccaaagct cccgcaaatt gtttggtaca ccgttaccgg
    24301 aactgttgag ctacttctca ttaaatatcg agctgatgca agaaagtctg gaggcggggc
    24361 aaggttttac cgataacgaa gtgacgctgg tcatcgacgg gcgctcgcat atcctttctg
    24421 tgacggccca gcgtatgccg gacggcatga tcctgctgga gatggctccg atggataacc
    24481 agcgccgctt aagtcaggaa cagctacagc acgcccagca ggttgctgcc cgtgatttag
    24541 tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg tttacgtggc gcggcgcagc
    24601 tactcagcaa agcgttacct gacccatcac tactcgaata taccaaagtg attatcgaac
    24661 aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg gccgcagctg cccggtacgc
    24721 gcgttaccga aagtattcac aaagtagctg aacgcgtggt aacgctggtg tcgatggaac
    24781 tgccggacaa cgtgcggttg attcgtgatt acgatcccag cctgccggaa ctggcgcacg
    24841 acccggatca aattgaacag gtcttgctga atattgtgcg caatgcgcta caggcgctgg
    24901 ggccggaggg tggtgaaatc attctgcgta cccgcaccgc gtttcaactg accttacacg
    24961 gcgagcgcta ccggctggcg gcgcggattg atgtggaaga taacgggccg ggcattccgc
    25021 ctcatttgca ggatacgctg ttttacccga tggtcagcgg ccgcgaaggt ggcaccgggc
    25081 ttggcttatc catcgcccgt aatttgattg atcagcattc aggcaaaatt gaatttacca
    25141 gttggccagg tcataccgag ttctcggttt acctgcctat caggaaataa aggtgacgtt
    25201 tatgcaacga gggatagtct gggtagtcga tgacgatagt tccatccgtt gggtgcttga
    25261 acgtgcgctc gctggagcgg gtttaacctg tacgacattt gagaacggcg cggaagtact
    25321 ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca gatatccgta tgccgggaat
    25381 ggacgggctg gcgctgctca agcagattaa acagcgccat ccaatgcttc cggtcatcat
    25441 tatgaccgca cattccgatc tggatgctgc cgtcagcgcc tatcaacaag gggcgtttga
    25501 ttatctgccc aaaccgtttg atatcgacga agccgtcgcg ctggttgagc gcgccatcag
    25561 tcattaccag gaacagcagc agccgcgtaa tattcagctt aacggcccaa cgaccgatat
    25621 catcggcgaa gcgccagcca tgcaggacgt gttccgtatt atcggtcggc tttcgcgttc
    25681 ttctattagc gtgctgatta acggcgaatc cggcaccggt aaagaactgg tcgctcatgc
    25741 cctgcatcgc cacagtccgc gcgccaaagc gccgtttatc gcgctgaata tggcagctat
    25801 cccaaaagat ttgatcgaat cagaactgtt tggccacgag aaaggcgcgt ttactggcgc
    25861 gaataccatt cgtcaggggc gttttgaaca ggccgatggc ggtacattat tcctcgacga
    25921 aattggtgat atgccgctgg atgtgcagac gcgtttgctg cgcgtgctgg cagacggtca
    25981 gttttaccgc gttggcggct atgcgccggt gaaagtggat gtgcggatta tcgctgccac
    26041 tcaccagaat ctcgaacagc gagtgcagga aggtaagttc cgtgaggatc tgttccaccg
    26101 cctgaacgtt atccgcgttc atctgccgcc gctgcgcgaa cgtcgggaag atattccccg
    26161 tctggcgcgc cattttttac aggttgccgc gcgcgaactg ggcgtagaag cgaagttact
    26221 gcatccggaa accgaagctg ctctgacgcg tctggcgtgg ccaggcaacg tgcgccagct
    26281 ggaaaacacc tgccgctggc taacggtgat ggccgccggg caggaagtgt tgattcagga
    26341 tttgcccggc gaactgtttg aatcaacggt tgcggagagt acttcgcaaa tgcaaccgga
    26401 cagctgggcg acgcttcttg cgcagtgggc agacagagcg ctgcgttccg gtcatcaaaa
    26461 tctgctttcc gaagcgcagc cagagctgga gcggacgtta ctgacgaccg cgttgcgaca
    26521 tacgcagggg cataaacagg aagcggcgcg gctactcggc tggggccgca acaccctgac
    26581 gcgtaagtta aaagagctgg ggatggagtg attcacagct tgtgtgtaaa gattgattat
    26641 tgagcgcaaa ttgctggtat tttacgcttt actgttccga taagttcagt atgatcttgc
    26701 ccggaaaacg gggagagtca ttatgctgga atcaataatt aatctggtat cgagtggcgc
    26761 agttgacagc cacacaccgc aaactgccgt tgctgccgtg ctgtgtgccg caatgattgg
    26821 gctgtttagt tgagtttcag attgctgatg aagtgctctt tgttcatgcc ggatgcggcg
    26881 tgaacgcctt atccggccta caaaagcaat attttcaaca ggttttgtag gcctgataag
    26941 cgtagcgcat caggcaatct tgttctcagc acattgatgg cgctttcgtt ttctactttg
    27001 taaacgaagc gccattcact atttaaatca cccgtgagaa ctgctgcatc cgcgctttct
    27061 ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat cagcaagcga cctttcgccg
    27121 tcacctgtat tcccttctca tccacatcca ccagcccatc ttttgctaac ggggcgagca
    27181 gcttgagatc ttccgcaaag taatcagcga agtgcaaatc ccactgtttc tcaatagggg
    27241 cgtaatccag acggaagttg cagatgagcg acttaatcac atcgcggcga atacagtcat
    27301 cacgcgttag cgcaatacca cgccacagcg cattgccttg ttcatccact tgctgatagt
    27361 actgcttcaa ctctttctgg ttctgcgcgt agcagtcgcc aatcatgctg atggcggaaa
    27421 cgcccatccc cagcagatcg gtatcgccct gagtggtgta gccctggaag ttacgatgca
    27481 gcacgccttc acgctgggcc accgccagct cgtcatccgg acgggcaaag tgatccatac
    27541 cgataaactg atagcccgat tgcgtcagga aggcgatggt ttcctgcagg atatcgagtt
    27601 tttgctgcgg actcggcagg tcagcatctt tgattttgcg ctgagcagca aaaatggtcg
    27661 gcagatgcgc gtagttaaag acactcagac gatcggggtt cagctccgcc acacgtttca
    27721 gggtaaaggc gaaactctcc ggcgtctgtt tcggcaggcc gtaaatcagg tcgatgttgg
    27781 tggaggtaaa accaatctca cgcgcatggt taagcagtgc aaagatgaac tcttcatcct
    27841 gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc ctgcacgccc atgctcaggc
    27901 gattaaagcc ttcggcgcgt aaatgatcga gtacatccag ttcgatttcc cgcggatcga
    27961 cttcgatcga aatctccgca tcggcattga actggaagtt ttcgcgcagc agcttcatca
    28021 ggcggctgat ttgcgcttta ttcagatacg tcggcgttcc gccgccccag tgcaattggc
    28081 tgacgtgacg cccggcgaac agcggtgcac gatggacgat ttcttgctcc agcgcgtcca
    28141 gatactgatc ggccttgtgc tgctggcgag taacaatctt attgcaaccg cagaagtaac
    28201 aaagcttatg gcagaacggg atatgtacgt agagagataa tggacgctca ggatagcgcg
    28261 ccacggcttg taaaaacgcc tgttcgccga agtcttctga aaactccagc gcggtcgggt
    28321 acgaggtgta tcgtggcccg gaatagttat atttctggat cagggccaga tcccagtcga
    28381 tttgctgtac agacatgctc actccttccg atggcgtctc tggcggctac ggcgtaccgg
    28441 cggatgcccg tttcgggtca ttagccggtt gcgcagccac ttttgtcgcc gcgacaaccg
    28501 ctgtagttta acgaataacc acaccagata acatataacc ggaagggtta tcagcagaac
    28561 gggaaggccc acggcggcaa tccgttagtt gccccgcagc agacgcatca tgtcttcttg
    28621 cttctcgtct tcttcctctt cttcgtcatc gtcataagag aggccgagtt tctgcatcaa
    28681 ctcatcaata cgatccagtt tagcatccac ccaggattgc tcttcggcac tcagggtttc
    28741 gcctgcttcc agacgttcca gcagcgcatc cagacgctca tccgtttcca gtaattccaa
    28801 ctccgcctgc ggtgaaagca taggtttctc actcttcggt ttgtgctgtt tggtgacttt
    28861 ttcagtcacg cccaatggaa taggggtttt actgccaata cgtggatctt ttggtgcgtt
    28921 ctggcctttg ctgcctgacg tggtgtttcc gcccgctgcg cggctgcccg gcgcatgacc
    28981 acgacgtttt ttctgacgct tgcgatcacg agcttcctga tccagctcct cgcgtgtttt
    29041 tcgacgcgct tttgcatgac ctttgctgcg tgagtttgaa gatgatggtt tcataatggt
    29101 tcgtctttta gccgttttat tcagtataga tttgcggcgg aatctaacag aaagcaagca
    29161 aagaaaaaag gcgacagatt actctgtcgc cttttttcct gactcataac ccttaacggg
    29221 tcttacaatc cttgcaggcc gccaacacac cctgtttttc ctttagccgg aacaatccgt
    29281 gttgattcca tttccgttta attacgtctc cggacgtttg tcttcctgac aatcctctgt
    29341 cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt gttcctgtta gcgtcctcgc
    29401 tctatgggtg ctactttacg cctttgcttt aaacaaacaa tcaaccgcca tcagtatttg
    29461 cacatt
//
LOCUS       DAZFWP020000053        29287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFWP020000053 DAZFWP020000000
VERSION     DAZFWP020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.053"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            137..1090
                     /gene="ybhD"
                     /locus_tag="ACHK0Z_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             137..1090
                     /gene="ybhD"
                     /locus_tag="ACHK0Z_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4502145.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKSLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            complement(1131..2126)
                     /gene="pgl"
                     /locus_tag="ACHK0Z_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             complement(1131..2126)
                     /gene="pgl"
                     /locus_tag="ACHK0Z_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU4502146.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2281..3099
                     /gene="ybhA"
                     /locus_tag="ACHK0Z_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             2281..3099
                     /gene="ybhA"
                     /locus_tag="ACHK0Z_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU4502147.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3100..4158)
                     /gene="modC"
                     /locus_tag="ACHK0Z_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             complement(3100..4158)
                     /gene="modC"
                     /locus_tag="ACHK0Z_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU4502148.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4161..4850)
                     /gene="modB"
                     /locus_tag="ACHK0Z_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             complement(4161..4850)
                     /gene="modB"
                     /locus_tag="ACHK0Z_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU4502149.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSILHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4850..5623)
                     /gene="modA"
                     /locus_tag="ACHK0Z_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             complement(4850..5623)
                     /gene="modA"
                     /locus_tag="ACHK0Z_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4502150.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5790..5939)
                     /gene="acrZ"
                     /locus_tag="ACHK0Z_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             complement(5790..5939)
                     /gene="acrZ"
                     /locus_tag="ACHK0Z_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU4502151.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6068..6856
                     /gene="modE"
                     /locus_tag="ACHK0Z_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             6068..6856
                     /gene="modE"
                     /locus_tag="ACHK0Z_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU4502152.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6924..8396
                     /gene="modF"
                     /locus_tag="ACHK0Z_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             6924..8396
                     /gene="modF"
                     /locus_tag="ACHK0Z_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU4502153.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDLYRYALTKIN"
     gene            8657..9673
                     /gene="galE"
                     /locus_tag="ACHK0Z_RS10650"
                     /old_locus_tag="pgaptmp_002130"
     CDS             8657..9673
                     /gene="galE"
                     /locus_tag="ACHK0Z_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4502154.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9683..10729
                     /gene="galT"
                     /locus_tag="ACHK0Z_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             9683..10729
                     /gene="galT"
                     /locus_tag="ACHK0Z_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU4502155.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10733..11881
                     /gene="galK"
                     /locus_tag="ACHK0Z_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             10733..11881
                     /gene="galK"
                     /locus_tag="ACHK0Z_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU4502156.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11875..12915
                     /gene="galM"
                     /locus_tag="ACHK0Z_RS10665"
                     /old_locus_tag="pgaptmp_002133"
     CDS             11875..12915
                     /gene="galM"
                     /locus_tag="ACHK0Z_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU4502157.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13120..13872
                     /gene="gpmA"
                     /locus_tag="ACHK0Z_RS10670"
                     /old_locus_tag="pgaptmp_002134"
     CDS             13120..13872
                     /gene="gpmA"
                     /locus_tag="ACHK0Z_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU4502158.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(14030..15082)
                     /gene="aroG"
                     /locus_tag="ACHK0Z_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     CDS             complement(14030..15082)
                     /gene="aroG"
                     /locus_tag="ACHK0Z_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU4502159.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15398..15778
                     /gene="ybgS"
                     /locus_tag="ACHK0Z_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             15398..15778
                     /gene="ybgS"
                     /locus_tag="ACHK0Z_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU4502160.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15892..16833
                     /gene="zitB"
                     /locus_tag="ACHK0Z_RS10685"
                     /old_locus_tag="pgaptmp_002137"
     CDS             15892..16833
                     /gene="zitB"
                     /locus_tag="ACHK0Z_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU4502161.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16830..17549)
                     /gene="pnuC"
                     /locus_tag="ACHK0Z_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             complement(16830..17549)
                     /gene="pnuC"
                     /locus_tag="ACHK0Z_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU4502162.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17587..18630)
                     /gene="nadA"
                     /locus_tag="ACHK0Z_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             complement(17587..18630)
                     /gene="nadA"
                     /locus_tag="ACHK0Z_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU4502163.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18879..18954)
                     /locus_tag="ACHK0Z_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     tRNA            complement(18879..18954)
                     /locus_tag="ACHK0Z_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18919..18921),aa:Lys,seq:ttt)
     gene            complement(18988..19063)
                     /locus_tag="ACHK0Z_RS10705"
                     /old_locus_tag="pgaptmp_002141"
     tRNA            complement(18988..19063)
                     /locus_tag="ACHK0Z_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19028..19030),aa:Lys,seq:ttt)
     gene            complement(19210..19285)
                     /locus_tag="ACHK0Z_RS10710"
                     /old_locus_tag="pgaptmp_002142"
     tRNA            complement(19210..19285)
                     /locus_tag="ACHK0Z_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19250..19252),aa:Lys,seq:ttt)
     gene            complement(19289..19364)
                     /locus_tag="ACHK0Z_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     tRNA            complement(19289..19364)
                     /locus_tag="ACHK0Z_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19329..19331),aa:Val,seq:tac)
     gene            complement(19514..19589)
                     /locus_tag="ACHK0Z_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     tRNA            complement(19514..19589)
                     /locus_tag="ACHK0Z_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19554..19556),aa:Lys,seq:ttt)
     gene            complement(19592..19667)
                     /locus_tag="ACHK0Z_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     tRNA            complement(19592..19667)
                     /locus_tag="ACHK0Z_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19632..19634),aa:Val,seq:tac)
     gene            complement(19803..19878)
                     /locus_tag="ACHK0Z_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     tRNA            complement(19803..19878)
                     /locus_tag="ACHK0Z_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19843..19845),aa:Lys,seq:ttt)
     gene            complement(20043..20834)
                     /gene="cpoB"
                     /locus_tag="ACHK0Z_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             complement(20043..20834)
                     /gene="cpoB"
                     /locus_tag="ACHK0Z_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU4502164.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20844..21365)
                     /gene="pal"
                     /locus_tag="ACHK0Z_RS10740"
                     /old_locus_tag="pgaptmp_002148"
     CDS             complement(20844..21365)
                     /gene="pal"
                     /locus_tag="ACHK0Z_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU4502165.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21400..22692)
                     /gene="tolB"
                     /locus_tag="ACHK0Z_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             complement(21400..22692)
                     /gene="tolB"
                     /locus_tag="ACHK0Z_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU4502166.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22825..24090)
                     /gene="tolA"
                     /locus_tag="ACHK0Z_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             complement(22825..24090)
                     /gene="tolA"
                     /locus_tag="ACHK0Z_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU4502167.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(24155..24583)
                     /gene="tolR"
                     /locus_tag="ACHK0Z_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             complement(24155..24583)
                     /gene="tolR"
                     /locus_tag="ACHK0Z_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU4502168.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24587..25279)
                     /gene="tolQ"
                     /locus_tag="ACHK0Z_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             complement(24587..25279)
                     /gene="tolQ"
                     /locus_tag="ACHK0Z_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU4502169.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25276..25680)
                     /gene="ybgC"
                     /locus_tag="ACHK0Z_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             complement(25276..25680)
                     /gene="ybgC"
                     /locus_tag="ACHK0Z_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4502170.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25830..26123)
                     /gene="ybgE"
                     /locus_tag="ACHK0Z_RS10770"
                     /old_locus_tag="pgaptmp_002154"
     CDS             complement(25830..26123)
                     /gene="ybgE"
                     /locus_tag="ACHK0Z_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU4502171.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(26123..26236)
                     /gene="cydX"
                     /locus_tag="ACHK0Z_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             complement(26123..26236)
                     /gene="cydX"
                     /locus_tag="ACHK0Z_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU4502172.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26251..27390)
                     /gene="cydB"
                     /locus_tag="ACHK0Z_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             complement(26251..27390)
                     /gene="cydB"
                     /locus_tag="ACHK0Z_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4502173.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27406..28974)
                     /gene="cydA"
                     /locus_tag="ACHK0Z_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             complement(27406..28974)
                     /gene="cydA"
                     /locus_tag="ACHK0Z_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU4502174.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7306 a         7733 c         7200 g         7048 t
ORIGIN      
        1 ttaagggcaa agcatctcta atgactttag ctcaacgatt aataacattt tttcattaat
       61 ttaagttggc taaatgcagt taaaattttt aacggccagc cacccaaaat cgtcgcttcc
      121 attttccgga gtaataatga agcatgaatt atcaagtatg aaggcatttg tcatactggc
      181 agagtccagt tcatttaaca atgccgctaa atcactcaat attacgcaac ctgcattaac
      241 gcgaagaata aaaaagatgg aggaagattt acatattcaa ctatttgagc gtacaactcg
      301 caaggttacg ttaacaaaag cagggaaaag gttgctcccg gaggcgcggg aattaataaa
      361 gaaattcgat gaaacgcttt ttaatattcg tgatatgaat gcttatcatc gtggtatggt
      421 gacgttagcg tgtattccga ccgcagtatt ttatttttta ccgctggcaa tcggtaaatt
      481 taatgagctg tatcccaata ttaaagtgcg gattctggaa caaggcacga ataattgcat
      541 ggaatcggtg ctgtgtaacg aatcagactt tggcataaac atgaacaacg tcacaaattc
      601 atccattgat tttaccccac tggtcaacga gccattcgtg ctggcctgtc ggcgtgacca
      661 tccattggcc aaaaagcagc ttgtagaatg gcaggagtta gtgggttaca aaatgattgg
      721 cgtacgttcc tccagcggca accgattgtt gatagaacaa cagctagccg acaagccctg
      781 gaagctggac tggttttacg aagtgcgtca tctttcgacg tcattaggac tagtggaggc
      841 aggactgggg atttcggcgc ttcctggtct ggcaatgccc catgcgccct attcttcgat
      901 tattggtatc ccactggtgg aacccgttat acgtcggaca ttggggatta ttcgccgcaa
      961 agatgccgtg ctttctccgg cagctgaacg cttttttgcc ttgctgatta atttgtggac
     1021 tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc cagagacatg cgttacagga
     1081 gataagttaa tacaccggcg caggagagac tgcgccggtt aaatcagcgg ttagtgtgcg
     1141 ttaaccacca cccacattgg tccctgcccg accgcataac ggcctttttc atgcagtagt
     1201 ccctgctcgc caacaatttc gtataccgag atgtggtgag atttttgccc ggcggcaatc
     1261 agatacttgc cgctgtgatc aacattgaag ccgcgcggct gggtttccgt tggctggaag
     1321 ccttctttac tcaacacgct gccatcttcc gaaacgctga aaacggtaat caggctggcg
     1381 gtacggtcgc aggcgtataa atggcgacca tccggggtga tatgaatatc agccgcccaa
     1441 cgggtgtcgg agaagttttc cggcatcata tccagcgtct ggacacattc gatattaccg
     1501 tgcggatctt tcagttccca gacatccact gagctgttta actcattgac gcaatacgca
     1561 tattgttcgt ttggatggaa taccatatga cgcgggccgg ccccttcaac ggtggtcact
     1621 tccgcagggt cctgcgccac gagatgacca tcatcgctga ccgtaaacag gcaaatgcga
     1681 tcctgcttta atgccggaac ccacagcgta cggttgtccg gtgagatatt ggcggaatgg
     1741 caaccgtcca gcccctcgac cacatcgacg acgcccactg gcaggccatc ttccagacgc
     1801 gttacgctca cgttacccgc attgtaagaa cctacaaaga caaactgccc ctggtgatcg
     1861 gtggaaatat gcgtcggact acccggcagc gcagactctg cggcaaaggt cagtgcgcca
     1921 tcgtccgggg caatacgata cgccaggacg cgaaactcag ggcgaacacc aacatagaga
     1981 taacgtttgt ccgggctgac caccatcggc tgcacctgcc ccggcacatc gacaacctgt
     2041 gtcagcgtca gtgcgccttc atgattcagg ttccagacgt gaatttgctg gctctcaggg
     2101 ctggcgatat aaactgtttg cttcatgaat gctcctttgc atttagcaac tgactgtaac
     2161 agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat
     2221 agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc
     2281 atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc
     2341 ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc
     2401 atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat
     2461 acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg
     2521 gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac
     2581 cacattcacg gtctgatgta tgtcgatgat gcaatggtct atgagcaccc gaccgggcat
     2641 gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca
     2701 caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg
     2761 acgcacgatg acctgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga
     2821 ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa
     2881 ggtaaacgtt tgacgaaatg ggttgaggcg caaggttggt cgatggaaaa cgtcgtggca
     2941 ttcggcgata actttaatga tatcagtatg ctggaagccg ctggtacagg cgtggcgatg
     3001 ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
     3061 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
     3121 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
     3181 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
     3241 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
     3301 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     3361 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cagtcatcgc
     3421 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     3481 cagccacgga ttcatcacgc tactacccca gacttcttcc agcgcgccaa aggctttcac
     3541 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     3601 gctgacatac aacatcggaa tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag
     3661 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     3721 agccgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     3781 cggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     3841 gaccatgctt ttcgccatgc cgtagcgcaa attgccacgc actttgtaat gcgggaacag
     3901 ccgtgcatcc tgaaaaacat agccaacgcg acgtttttct ggcgtcaggc agataccttt
     3961 ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag
     4021 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     4081 gatgccattg gcgggcagcg tttcattaat agtcaggcaa tggttgccca aggtctggga
     4141 aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     4201 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgctgcg
     4261 ccactttccc cgccgggggt ctgaatcagg gtatacatgg cagaaggaat ggttcgcgtt
     4321 tcaccaggaa tgttcgacac aaaggtgatg gttgcgccaa attcaccgag agaacgagca
     4381 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     4441 aaaacgcgcc agcgcccggc ccccagtgtt cttgcggcct gttccagttt gacatcaacc
     4501 ccttccagcg ccagacgaat tgcccgcacc attagcggaa acgacatgac ggcggcagcg
     4561 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     4621 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     4681 aacaccagcg gtagatgcag tatgctgtcg agcaaagctt tgcccggaaa cgtgcaacgc
     4741 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     4801 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     4861 taaatccgta acgtttaaag atttccgcag cctgcggtcc cttcagataa tcataaaatg
     4921 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     4981 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctggcaact gcgtcagaac
     5041 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     5101 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     5161 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     5221 tcagcagcga agtccagtta gttttgctgt cgatgatgaa atctttctgc tcgctggctt
     5281 tcggtgctac aacgaccagg ctattgccga gcagtgtctg acgcgtagct gtatcgatcg
     5341 cttttttatc aactgcataa tccatccatt tctgatcggc agaaataaac agatccgcag
     5401 gcgcacccgc ttcaatctga cgggcgagag tagatgacga agcgaaagaa gaaaccacat
     5461 ccacgccttt ctctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     5521 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgc attgccagca acagcgaaag
     5581 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     5641 aacgttatgt aggcgacaat ataacgacta actcggaatt ttcccagcag ttattactaa
     5701 cctttgtgag gtagataaga aaaaatatcg gcaggaaaag caggaagttg agaaaaagaa
     5761 aatgcccgac taagcgggca ttcagggaat caatgatttt gtccgggctg gtctttttta
     5821 ccaacaccag aaaagatgtt gaatacttca ccaagaccgt aaatcagacc caggatgatg
     5881 gccatcacga caggtaccat gattacggcg aataccagac tttttaataa ctctaacatg
     5941 gtcaactcca gatatagtca tgagactatt ctaaccgcta agcacagaaa agcactcccc
     6001 ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca caataagact tttgccagga
     6061 cattgttatg caggccgaaa tccttctcac ccttaagctc caacaaaaat tattcgccga
     6121 cccgcgccgc atttcgctac taaaacacat tgcgctttcc ggttccatta gccagggagc
     6181 gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc attaacgaga tgaatcagtt
     6241 aagtgagcat attctggtcg agcgcgcaac aggcggtaaa ggtggcggcg gcgcagtact
     6301 gacccgctat ggtcagcgac tgattcagct ctatgactta ctggcgcaaa tccagcaaaa
     6361 agcctttgat gtgttaagtg acgatgacgc cctgccgctg aacagcctgc tggccgcgat
     6421 ctcacgtttt tcactgcaaa ccagcgcccg taaccagtgg ttcggcacca tcaccgcccg
     6481 cgatcatgat gacgttcaac agcatgttga tgtcttactg gctgacggaa aaacacgcct
     6541 gaaagtcgca attaccgcac aaagcggcgc acgtctgggg ctggatgaag gcaaagaagt
     6601 gttgatattg ctaaaagcgc cgtgggtagg cattactcag gacgaagcgg tcgcacaaaa
     6661 cgccgacaac caattaccgg gtattattag tcatattgag cgcggcgcag agcagtgcga
     6721 agtattaatg gcgctacccg acgggcaaac actgtgcgcc acagtgccgg ttaatgaagc
     6781 gacttctctt cagcaaggac agaatgtcac ggcctacttt aatgccgaca gcgtgattat
     6841 cgccacgcta tgctaagcgt gttgacaatt tgttatgaaa cacgtatccc tatcagtaat
     6901 cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat tttgcaaggc acgtttcgtc
     6961 ttagcgacac aaaaacgctg caattgcctc agctaacgtt aaacgcgggt gatagttggg
     7021 cgtttgtcgg ttcgaatgga agtggaaagt cggcgctggc ccgtgcgctg gcgggggaac
     7081 ttccgctttt gaaaggtgaa cggcaaagcc agttttccca catcactcgt ctctccttcg
     7141 agcaattgca aaagctcgtc agcgacgaat ggcagcggaa taacaccgat atgctcggcc
     7201 ctggcgaaga tgacaccgga cgcactacgg ctgagatcat tcaggatgaa gttaaggatg
     7261 caccgcgttg catgcaactg gcgcagcagt tcggtattac cgccctcctc gaccgacgct
     7321 ttaaatacct ttccactggc gagacgcgaa aaaccctgct gtgtcaggcg ctgatgtcgg
     7381 agcctgactt gttgattctt gatgagccgt tcgatggcct ggatgttgcc tcacgtcagc
     7441 agctggctga gcgactcgcc tcgttacatc agtccggtat tactctggta ctggtgctca
     7501 atcgcttcga tgagatcccg gaatttgtcc agtttgctgg cgtgctggcg gattgtacgt
     7561 tagcggaaac gggcgctaaa gaggaactgc tccagcaagc actcgtcgcg caactggcgc
     7621 atagcgaaca gcttgaaggt gtgcaactgc cggagccgga tgaaccttca gcacgtcacg
     7681 ccttacccac caacgaaccg cgcattgtgc tgaataatgg cgtggtttct tataacgatc
     7741 gccccattct taataacctt agctggcagg tgaatcctgg cgagcactgg caaattgtcg
     7801 ggccaaatgg tgcgggaaaa tcgacgttat taagcctgat tactggcgat catccgcaag
     7861 gttacagcaa cgatttgacg cttttcggcc gacgtcgcgg cagcggcgaa accatctggg
     7921 atatcaaaaa gcatatcggt tacgtcagca gtagtttgca tctggattac cgggtcagca
     7981 ctaccgtgcg taatgtgatt ctttctggct attttgattc gattggcatt tatcaggccg
     8041 tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga tattctcggc attgataaac
     8101 gcacggctga cgttccgttc catagtcttt cctggggaca gcagcgtctg gcgctgatcg
     8161 tccgcgcgct ggtgaaacat ccgacattgc ttattctcga tgaaccacta caggggcttg
     8221 atccgctcaa tcgccaactt atccgccgtt ttgttgatgt gctgattagc gaaggtgaaa
     8281 cgcaattgtt gtttgtttcg caccacgctg aagatgcgcc tgcctgtatt acccatcgtc
     8341 ttgagttcgt gccggacggt gacctttatc gctatgcgct gacaaaaata aactgagttg
     8401 gtaatgctga ctgtgccgga tgtggcgtaa gccccctctc cggcctacag ttcgacgcat
     8461 gcaggcatga aaccgcgtct tttttcagat aaaaagcgca atcattcata aaccctctgt
     8521 tttataatca cttaatcgcg cataaaaaac ggctaaattc ttgtgtaaac gattccacta
     8581 atttattcca tgtcacactt ttcgcatctt tgttatgcta tggttatttc ataccataag
     8641 cctaatggag cgaattatga gagttctggt taccggtggt agcggttaca ttggaagtca
     8701 tacctgtgtg caattactgc aaaacggtca tgatgtcatc attcttgata acctctgtaa
     8761 cagtaagcgc agcgtactgc ctgttatcga gcgtttaggc ggcaaacatc caacgtttgt
     8821 tgaaggcgat attcgtaacg aagcgttgat gaccgagatc ctgcacgatc acgctatcga
     8881 caccgtgatc cacttcgccg ggctgaaagc cgttggcgaa tcggtacaaa aaccgctgga
     8941 atattacgac aacaatgtca acggcactct gcgcctgatt agcgccatgc gcgccgctaa
     9001 cgtcaaaaac tttattttta gctcctccgc caccgtttat ggcgatcagc ccaaaattcc
     9061 atacgttgaa agcttcccga ccggcacacc gcaaagccct tatggcaaaa gcaaattgat
     9121 ggtggaacag atcctcaccg acctgcaaaa agcccagccg gactggagca ttgccctgct
     9181 gcgctacttc aacccggttg gcgcgcatcc gtcgggcgat atgggtgaag atccgcaagg
     9241 cattccgaat aacctgatgc catacatcgc ccaggttgct gtaggccgtc gcgactcgct
     9301 ggcgattttt ggtaacgatt atccgaccga agatggtact ggcgtacgcg attacatcca
     9361 cgtaatggat ctggcggacg gtcacgtcgt ggcgatggaa aaactggcga acaagccagg
     9421 cgtacacatc tacaacctcg gcgctggcgt aggcagcagc gtgctggacg tggttaatgc
     9481 cttcagcaaa gcctgcggca aaccggttaa ctatcatttt gcaccgcgtc gcgagggcga
     9541 ccttccggcc tactgggcgg acgccagcaa agccgatcgc gaactgaact ggcgcgtaac
     9601 gcgcacactc gatgaaatgg cgcaggacac ctggcactgg cagtcacgcc atccacaggg
     9661 atatcccgat taaggaacga ccatgacgca atttaatccc gttgatcatc cacatcgccg
     9721 ctacaacccg ctcaccgggc aatggattct ggtttcaccg caccgcgcta agcgcccctg
     9781 gcagggggcg caggaaacgc cagccaaaca ggtgttacct gcgcacgatc cagattgctt
     9841 cctctgcgca ggtaatgtgc gggtgacagg cgataaaaac cccgattaca ccgggactta
     9901 cgttttcact aatgattttg cggccttgat gtctgacacg ccagatgcgc cagaaagcaa
     9961 cgatcctttg atgcgttgcc agagcgcgcg cggtaccagc cgggtgatct gcttttcacc
    10021 ggatcacagt aaaacgttgc cagaactgag cgttgcggca ttgacggaaa tcgtcaaaac
    10081 ctggcaggag caaaccgcag agctgggaaa aacgtacccg tgggtgcagg tctttgaaaa
    10141 caaaggtgcg gcgatgggct gctctaaccc gcatccgcac ggacaggttt gggcaaatag
    10201 cttcctgcct aacgaagctg agcgcgaaga ccgcctgcaa aaagaatatt ttgccgggca
    10261 gaaatcgcca atgctggtgg attatgttca gcgcgagctg gcagacggta gccgtaccgt
    10321 tgtcgaaacc gaacactggt tagccgtagt gccttactgg gctgcctggc cgttcgaaac
    10381 gctactgctg cccaaagccc acgttttacg gatcaccgat ttgaccgatg cccagcgcag
    10441 cgatttagca ctggcgttga aaaagctgac cagtcgttat gacaacctct tccagtgctc
    10501 cttcccctac tctatgggct ggcacggcgc gccatttaat ggcgaagaga atcaacactg
    10561 gcagctgcac gcgcactttt atccgcctct gttgcgctcc gccaccgtac gtaaatttat
    10621 ggttggttat gaaatgctgg cagagaccca gcgagacctg accgcagaac aggcagcaga
    10681 gcgtttgcgc gcagtcagcg atatccattt tcgcgaatcc ggagtgtaag aaatgagtct
    10741 gaaagaaaaa acacaatctc tgtttgccaa cgcatttggc taccctgcca ctcacaccat
    10801 tcaggcgcct ggccgcgtga atttgattgg tgaacacacc gactacaacg acggtttcgt
    10861 tctgccctgc gcgattgatt atcaaaccgt gatcagctgt gcgccacgcg atgaccgtaa
    10921 agttcgcgta atggcagccg attatgaaaa tcagctcgac gagttttccc tcgatgcgcc
    10981 cattgtcgcg catgaaaact atcaatgggc gaactacgtt cgtggcgtgg tgaaacatct
    11041 gcaactgcgt aacaacagct tcggcggtgt ggacatggtg atcagcggca atgtgccgca
    11101 gggggccggg ttaagttctt ccgcttcact ggaagtcgcg gtcggaaccg tattgcagca
    11161 gctttatcat ctgccgctgg acggcgcaca aatcgcgctt aatggtcagg aagcagaaaa
    11221 ccagtttgtt ggctgtaact gcgggatcat ggatcagcta atttccgcgc tcggcaagaa
    11281 agatcatgcc ttgctgattg actgccgctc actggggacc aaagcagttt ccatgccgaa
    11341 aggcgtagct gtggtcatca tcaacagtaa cttcaaacgt accctggttg gcagtgagta
    11401 caatactcgc cgtgagcagt gcgaaaccgg tgcgcgtttc ttccagcagc cagctctgcg
    11461 cgatgtcacc attgaagagt tcaacgctgt tgcgcatgaa ctggacccga tcgtggcgaa
    11521 acgcgtgcgt catatcctga ccgaaaacgc ccgcaccgtt gaagctgcca gcgcgctgga
    11581 gcaaggcgac ctgaaacgta tgggcgagtt aatggcggag tctcatgcct ctatgcgcga
    11641 tgatttcgaa atcaccgtgc cgcaaattga cactctggta gaaatcgtca aagctgtgat
    11701 tggtgacaaa ggtggcgtac gcatgaccgg cggcggattt ggtggctgta tcgtcgcgtt
    11761 gatcccggaa gaactggtgc ctgccgtaca gcaagctgtc gctgaacaat atgaagcaaa
    11821 aacaggtatt aaagagactt tttacgtttg taaaccatca caaggagcag gacagtgctg
    11881 aacgaaactc ccgcactggc acccgatggt cagccgtacc gactgttaac tttgcgtaac
    11941 aacgcaggga tggtagtcac gctgatggac tggggtgcga ctttactttc cgcccgtatt
    12001 ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct gtgccagccc ggaatgctat
    12061 caggatcagg ctgcgtttct gggggcctct attggtcgtt atgccaaccg catcgccaat
    12121 agccgttata cctttgacgg tgaaaccgtg acgctttcgc caagtcaggg cgttaaccag
    12181 ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc agattgtgaa ccagaacgat
    12241 cgtcaggtgc tgtttgccct gagttcagat gatggcgatc agggcttccc gggtaatctc
    12301 ggcgcgacgg tgcaatatcg tctgaccgac gataaccgta tctctattac ttatcgcgcc
    12361 acagttgata aaccttgccc ggtgaatatg actaatcacg tctatttcaa tcttgacggc
    12421 gagcagtctg acgtgcgcaa tcacaagttg cagattctgg cggacgaata tctgccggtt
    12481 gatgaaggcg gcattccgca cgacggcctg aaatctgtcg ccggaacgtc ttttgatttc
    12541 cgtagcgcca aaatcatcgc cagtgagttt cttgccgacg acgatcagcg caaagtgaaa
    12601 ggttacgatc acgcattctt gttacaggcc aaaggcgatg gcaagaaagt ggcggcgcat
    12661 gtctggtcag cagatgaaaa attgcagctg aaggtctaca ccaccgctcc ggctctgcaa
    12721 ttctactccg gcaacttcct cggcggcacg ccatcaagag gaactgaacc ttacgccgac
    12781 tggcaaggcc tggcactgga aagcgagttt ctgccggaca gcccaaacca ccctgaatgg
    12841 ccgcaaccgg actgcttcct gcgtcctggc gaagagtatt ccagcctgac ggaatatcag
    12901 tttattgctg agtagcacct gaagccctcg taatgagggc tttatcctta tattgctcaa
    12961 atcaccagca aacaccgaca tatttgcaac tcaatattca caacaacctt acactgcgac
    13021 actattttcg ctatggttat gcgtaagcat tgctgttgct tcgtcacggc aatataatga
    13081 gaattattat cattaaaaaa tgatttgagg agtaagtata tggctgtaac taagctggtt
    13141 ctggttcgtc atggcgaaag tcagtggaac aaagaaaacc gtttcaccgg ttggtacgac
    13201 gtggatctgt ctgagaaagg cgtaagcgaa gcaaaagcag caggtaagct gctgaaagag
    13261 gaaggttaca gctttgactt tgcttacacc tctgtgctga aacgcgctat ccataccctg
    13321 tggaatgtgc tggacgaact ggatcaggca tggctgcccg ttgagaaatc ctggaaactg
    13381 aacgaacgtc actacggtgc gttgcagggt ctgaacaaag cggaaactgc tgaaaagtat
    13441 ggcgacgagc aggtgaaaca gtggcgtcgt ggttttgcag tgactccgcc ggaactgact
    13501 aaagatgatg agcgttatcc gggtcacgat ccgcgttacg cgaaactgag cgaaaaagaa
    13561 ctgccgctga cggaaagcct ggcgctgacc attgaccgcg tgatccctta ctggaatgaa
    13621 actattctgc cgcgtatgaa gagcggtgag cgcgtgatca tcgctgcaca cggtaactct
    13681 ttacgtgcgc tggtgaaata tcttgataac atgagcgaag aagagattct tgagcttaat
    13741 atcccgactg gcgtgccgct ggtgtatgag ttcgacgaga atttcaaacc gctgaaacgc
    13801 tattatctgg gtaatgctga cgagatcgca gcgaaagcag cggcggttgc aaaccagggt
    13861 aaagcgaagt aaacgtcatt cgtttaaaat gagaaagccg actgcaagtg agtcggcatt
    13921 tttgttgcta acaatggagc acattgcctg atgcgacgct gcgcgactta tcaggcctgt
    13981 ggtgattcat cggatacgcc actctgacgg cgcatccgac aattaaacct tacccgcgac
    14041 gcgcttttac tgcattcgcc agttgacgta acagagcatc ggtatcttcc cagccgatgc
    14101 aggcatcggt gatgctctta ccgtaggcca gcggctcccc gctctcgagg ctctgattgc
    14161 cttccaccag atggctttcc accatcacgc caataatggc cttttcgcca ccggcaatct
    14221 gctggcaaac gtcagcacaa acatccatct gctttttgaa ttgtttggac gagttagcat
    14281 ggctgaaatc gatcatcacc tgtgctggca ggcctgcttt gttcagccct tctttcactt
    14341 cagcaacgtg cttcgcgctg tagttaggct ctttaccgcc gcgcagaatg atatggcaat
    14401 cgccgttacc gctggtattc acaatcgccg aatgccccca tttcgttacg gacaggaagc
    14461 agtgcggcgc accggcggca ttaatggcat cgatagccac tttaatcgta ccgtcggtgc
    14521 catttttgaa gccgaccgga caagaaagcc ctgatgccag ttcgcggtgc acctgcgatt
    14581 cggtggtacg tgcgccaatt gcgccccagc tcatcaggtc agcgagatat tgtggggtga
    14641 tcatatcgag aaactcacct gccgctggca gaccgctgtc gttaatatca agcagcaatt
    14701 tacgggctat acgcagaccg tcgttgatct ggaagctatt atccatatgc ggatcgttaa
    14761 tcagcccttt ccagcccacc gtggtacgcg gcttttcaaa atagacgcgc attacgattt
    14821 ccagctcatc tttcagctct tcacgcagcg ccagcaagcg agtggcatac tcttttgccg
    14881 cgacaggatc atgaattgag catgggccaa tcacaaccaa caggcgatca tcattacctt
    14941 tcaggatctt atggatcgct tttcgggcat gggcaaccgt attcgcggca ttttcagtag
    15001 cggggaattt ttccagcaat gcgacaggag gaagtaactc tttgatttct ttgatgcgta
    15061 aatcgtcgtt ctgataattc atgtctgttc cagtgttgcc atacttatct tagtgaatgc
    15121 aatacttcca atctatatct tccgtcagaa tgtgtaaacg gggttttaca ctatgaacgg
    15181 attaatcctg gaattagcaa gaaaaacgcc agattgtcga gaaaaacgag atctctccta
    15241 caatttctaa ctgtaactcc tttgtttgtt aattatttca agattctctg ctgcgtttca
    15301 taacctggct gaaatcttaa accaatgcct tatattcacc tgcaaatgca ctgttggaag
    15361 aggttatccg acataacgac cataacagga gcatcctatg aaaatgacaa aactggccac
    15421 attatttctg actgccactc taagccttgc cagcggtgcc gcactggctg ccgatagcgg
    15481 agcgcaaact aataacggcc aggcaaacgc cgcagctgat gcgggccagg tagcccctga
    15541 cgcccgtgaa aatgtcgcgc caaataacgt cgacaataac ggggtaaata ccggttctgg
    15601 tggcacaatg ctgcattcgg atggttcttc aatgaacaat gacggaatga ccaaagatga
    15661 agagcacaag aacaccatgt gcaaagatgg tcgctgtccg gatatcaata aaaaggtaca
    15721 aaccggtgat ggcatcaata atgatgtcga taccaaaacc gacggtacca cgcagtaacc
    15781 ctgcaaaata gccaataatg ggagagcgga gtctctccct tttaattttt cacttctaaa
    15841 agcagtatga tgtcataaag ttaagtgaga ataattaagg gatgactcgt catggcgcac
    15901 tcacactcac acacgtcctc acacctgcca gaagataata atgctcgtcg cttgttgtat
    15961 gctttcggcg tgactgccgg gtttatgctg gtagaagtcg ttggtggttt tctttctggt
    16021 tctctggcat tgctggccga tgcgggtcat atgttgaccg atactgccgc cctgcttttc
    16081 gccctactcg ccgtgcaatt ttcacgtcgt cctcccacca ttcgccacac tttcggttgg
    16141 ctaagactga ccacgctcgc ggcgtttgtg aatgccatag ccctcgtggt aattaccatt
    16201 ttgattgtct gggaggcgat agaacgcttc cgcacgccgc gtccggtcga gggcggcatg
    16261 atgatggcaa ttgccgtggc tgggttgctg gcaaatatac tttctttctg gttacttcat
    16321 cacggcagcg aagagaaaaa cctcaacgta cgagcagcgg cactgcatgt gctcggggac
    16381 ctgctgggtt cggttggggc tattattgcc gcgttgatta ttatctggac tggctggaca
    16441 cctgctgacc ccattctctc gatactggtg tcgcttctgg ttctgcgtag cgcgtggcga
    16501 ctgttgaaag atagcgtgaa tgaattactt gaaggtgcac cggtatcgct ggatatcgct
    16561 gaactgaagc gtcgtatgtg ccgggaaatc ccggaagtcc gcaatgtgca ccatgtacat
    16621 gtatggatgg taggcgagaa gccggtgatg acgttgcatg tgcaggtgat cccgccacac
    16681 gatcacgatg ccttgttgga tcagatccaa cactacctga tggatcacta tcagattgag
    16741 cacgccacca ttcagatgga atatcaacct tgtcatgggc cagactgcca cctgaatgaa
    16801 ggggtgtctg gccattcaca tcatcaccat taatgggaca gcgcgcgtga gcctctttca
    16861 cgtgcgctgt tgatccacat ccggctgccg ttgagcgcaa taaaggtcag gatgatgtac
    16921 tccagagaca tggcgtaaac gccctgaagt gcaaaaataa cgacgctaat cacgttaata
    16981 atcacccaca acagccagtt ttccacatac ttacgcgtca tcagaatcat tgccacgata
    17041 gataacacca tcatgcatga atcccagaac gggaaagcgt ccggttgcag ttcaggcatc
    17101 acaacctgta atcctaatgc ttgcatgatc atgactgcca cgcgggtcaa aaatgcaaac
    17161 accggattga taaataccgt catcagacca atcgaaacaa cgcaaaccgc caaccagctg
    17221 agtgccttcg gcaatggcaa ccagcgaatt ttcaactccg cctcgttctg actggtttgt
    17281 cgcgaccacg cataccaacc gtaaatattc gcggcaaaga aaaacacctg taatagcagg
    17341 ctggcataca gctgaatctg aaagaaaata atgccaaaca aggtgacgtt gatcaggcca
    17401 aagaagtagt tgctgatctt ctccagactg gcaaggccaa tacacagcaa cccggcgatc
    17461 gtgcctaccg cttcgatcca tgagagatca taaccgcctg cccctattgg tatatgtacc
    17521 aggatattct gcacactaaa aaaatccatc tttcccccaa agcgtaacgt tacgccttat
    17581 tattcgttat ccacgtagtg tagccgcaaa atccagcata cgattgagcg gcaccagcgc
    17641 cctctctcgc agcctttcat caacatgaac ctcgtgattg cttccttcct gttctaatgc
    17701 ctctgcgatg gactgaaggc cattcatggc catccacgga caatgcgcgc agctgcggca
    17761 ggttgcaccc tcacctgcgg ttggtgcttc cagtaactct ttatctggca ctgcctgctg
    17821 cattttgtag aaaatgcccc gatcggttgc cacgataagc cgctgatgtg gcaatgtttt
    17881 cgcagcagcg atcagttgac tggtggaacc gaccgcatcc gccatatcga caatagcttg
    17941 tggtgattct ggatgcacca gtatggcagc atccgggtat tcttcttgca agcgggttaa
    18001 cgcctgagtc ttaaattcat catgcacaat acaggcaccc tgccagcata gaatgtctcc
    18061 acccgtctgt ttttgcacgt aacgccccag atgtttgtcg ggtgcccaga tgattttttc
    18121 acccaaacta tcaagatgat caataagttc gacggcaatg cttgaagtta ccacccaatc
    18181 tgcgcgcgct tttaccgcag cagaagtgtt ggcgtagacg acgacagtac gatcgggatg
    18241 ggcatcgcaa aatgcgttaa attcttcaac agggcagccg agatccagtg aacattcagc
    18301 ctgaagtgtc ggcatcagaa ttgttttttc cggactgaga attttggcgg tttctcccat
    18361 aaatctcacc ccagcgacta acaaagtaga agcgggatgc tttgcaccga agcgcgccat
    18421 ttccagagaa tcagaaatac agccaccggt ttcttctgcc agttgttgaa tttcgggatc
    18481 ggtatagtag tgggcaacca tcaccgcatt acgttctttt agcagacgtt ttatcttctc
    18541 gcggtaatac gctttttcat caatgcttaa cggcgtcggc ttcgggggga aaggataaat
    18601 cgccgtgtct ggatcaaaca ttacgctcat cttaccatct cgttttacag gcttaacgtt
    18661 aaaaccgaca ttagcgtaat attcgctgtt atcggtgtaa tttgttttat atgctaaaca
    18721 agatagtcga atggtgggga aaagtcactc gaattacgcc taattccacc gacgtagagt
    18781 tgcttgcaca gtaagctctg acgatgtcat caggtttaca aagtgaaatt gaggatgcct
    18841 gaatagcaaa aaagcgcctt tagggcgctt ttttacattg gtgggtcgtg caggattcga
    18901 acctgcgacc aattgattaa aagtcaactg ctctacaaac tgagctaacg accccttgcg
    18961 ggattatgct gctttaatca ttcaggatgg tgggtcgtgc aggattcgaa cctgcgacca
    19021 attgattaaa agtcaactgc tctaccaact gagctaacga cccatacggg tgttacctga
    19081 aagatttcta ctcggggcca atctttaaga ttggtgtgtc gtgcaggatg actcgcttcg
    19141 cttgccctac gggccgttgc tggcgcaacg ttatccttca cgcttaacat ctgagtttga
    19201 tgttaaaatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact
    19261 gctctaccaa ctgagctaac gacccgagtg gtgggtgatg acgggatcga accgccgacc
    19321 ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgatact gcgctggata
    19381 ctacaaacta cttccaccga acccaccgta attagtggtg ggtcgtgcag gatgactcgc
    19441 ttcgctcgcc ctacgggccg ttgctgacgc aacgttatcc tacacgctta acatctgtct
    19501 tcgatgctaa aactggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc
    19561 aactgctcta ccaactgagc taacgacccg gtggtgggtg atgacgggat cgaaccgccg
    19621 accccctcct tgtaagggag gtgctctccc agctgagcta atcacccaat actacgctgg
    19681 atactacaac taactatgtt agtggtgggt cgtgcaggat gactcgactt cgtctcgccc
    19741 ttcgggccgt tgctgatgca acgttatcct tcacgtttta ctaccgcttc caccttagcg
    19801 attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac
    19861 caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc ccgtggcaac
    19921 ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg cagatcactc
    19981 aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc gtgtgttatg
    20041 cattacatcg cgttcagacg tttttgtgcc tgtttagcgc catcagtacc agggtattta
    20101 ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg
    20161 acgccgactt taaacatcgc atcagcagcc tttggtgact tcggatagtt tttcactacc
    20221 gaagcaaaat agtacgccgc atcatctttt ttacccttgt tgtagtttaa ctgacccagc
    20281 caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa attctgaaat
    20341 gccaccattg cgtcatcctg gcgggattta tcctgcacca gcgcaatagc tgcattgtaa
    20401 tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc agcatcagct
    20461 gtcggcgtcg ttgatgccgc cgcaccgctt tgatcgctgc tggttgattg cgccgctgca
    20521 ccaccgctgc tgaggctgtc gatctgcaac aggatctgct tttgccgctc cacgacctga
    20581 ttcagttgat actgattttc ctgaatctga ccacgcaggg aatcaatatc ggattgatta
    20641 tcagaaagtt gttgctggag ttgggttaaa agctggctgt gagcattaga aatacgctca
    20701 agttgagtga cgcggtcttc gaccgagcct gagccgacac tactgattgg tgcctgagca
    20761 aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag ttgatgtctg
    20821 aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt tttggagtat
    20881 gccgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac gatggagatc
    20941 tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc acgacgttca
    21001 cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc tacggtgact
    21061 ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg agcgaagtca
    21121 gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg cagctgttgc
    21181 atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt cgcatccata
    21241 ccagtgccgg cacccagcat gccttcgctg ccgtcattgc tggcgttctt gttggaagaa
    21301 catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac tttgttcagt
    21361 tgcatttcaa tgattccttt actattcaat taattattat cacagatacg gcgaccaggc
    21421 agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc catctgtaga
    21481 aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca tagtgccgtt
    21541 aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta cgcctcccgt
    21601 tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca ttaccataaa
    21661 tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg taatacgttg
    21721 tggcgcaccg ccgttgatat tcactttata aacctgcgga cgaccggcct ggtcagaagt
    21781 aaatgccagg ttctggctat ccgggaacca ggtcggttcg gtattgttac tgcgaccatc
    21841 agtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca gactaccggt
    21901 tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg
    21961 acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtctgaa taaccagcgc
    22021 ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg accacgccgg
    22081 agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt catagtcaga
    22141 tacgcgcagt tcatacggga actgaccgcc gttggtctga acaacgtagg caatacgagt
    22201 acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg cggtatgacc
    22261 agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta cagtacccgg
    22321 tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg gattcggagt
    22381 gacctgaccg acaactacag cgtcaatgcc cagtgcggac catgcagctg gttgtacttc
    22441 ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa atttaccgct
    22501 gttacgcaag tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac caggccccgc
    22561 ccactggaaa ggaacaacac caataggacg accggaatct acaccgctgt cgatcacaat
    22621 gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa
    22681 tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt tagcagaatg
    22741 ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc ctgatgttga
    22801 ccgtccgaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac
    22861 ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg ctgccaacgc
    22921 agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca taccatcggg
    22981 tgccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt catagaactt
    23041 actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc ctgatgcgcc
    23101 attgttttta gtattaccac tcccggcagg cgaagcattg ttccctttcg cccctccccc
    23161 cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg cctctgcggc
    23221 cgcttttgct gcagcggctt tttcagctgc agcttttgcc gctgccgctt ttttgtctgc
    23281 ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttttcagcag ccgctttttc
    23341 tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag ctgcagcctt
    23401 ttcagcagcc gctttcttct cagcttctgc tttggctttt tcagcagctt cagttgccgc
    23461 tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct tcagtgccgc
    23521 agcggctgcc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc
    23581 ttttttcttt gcgtctgcag ccgctttctt cgctgcttct tccgcagctt tagcatctgc
    23641 ttcggccttc gctttagcat ctgccgccgc tttcgctgcc gcctcttcag cttgcttctg
    23701 ctttaactcg gcctgttttg cggcttcttc agcctgcttt ttctgctcct gagccgctaa
    23761 ccgctctttc tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc
    23821 ttcagcagcc tgctgttcct tcatcttgcg ctgttcatca gaacgcttcg cgcttgattc
    23881 ctggctttgc atgcgtttgt actgctcaac taccgcacct gaatcaacca tgacagcgtc
    23941 gatggacgaa ccaccgccgc ctccggctga agcttctata ttctcatcga acgaactcca
    24001 gatcagcgcc gcaaataaga tgacatgcag cactgctgaa ataattatcg cccgcttgag
    24061 cttgtcgttt tgttcggttg cctttgacac tctcggtttc caaaaactgt tcgcctgtta
    24121 cccgctctct ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc attaaaccaa
    24181 ccgatttcac acccgcacta tgtaacaagt tcagtgcttt aattatttca tcgtaaggca
    24241 catcttttgc gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa cggctggaca
    24301 cttccgccac cacctgctct ggtggtaaac gctccaggcg atctttctca accaccacgg
    24361 tgtactgacc aataccagac acttcaacaa tcactggcgg attatcgtta ctgctcaccg
    24421 cctgtgattc agtagcgtct ggcagatcga cctccacgct ctgggtgatg atgggcgctg
    24481 tcgccataaa gatcagcaac agcaccagca gtacgtccag caacggtaca atgttgattt
    24541 cggacttgag atcgcgacga cctcgtccac gcgctctggc catggcttac cccttgttgc
    24601 tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc ataaagttgt
    24661 cgtaattcag ttccagtttg tttacgcgct ggttgaggcg gttataagcc attaccgccg
    24721 gaattgcggc gaacagacca attgcagtcg caatcaacgc ttctgcgata ccgggcgcaa
    24781 ccatttgcag tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc atgatccccc
    24841 agaccgtacc aaacagacca atatacgggc tgatggagcc aaccgtaccg aggaacggaa
    24901 tgtgcgtttc cagattttca agttcacggt tcatggagat acgcatagca cgcgacgccc
    24961 cttccactac ggcttccggc gcatggctat tggcacgatg caggcgcaca aactctttga
    25021 acccgctgta aaagatttgt tccgaaccag tcagattatc ccgtttcccc tggctctctt
    25081 gatagaggcg agagagttcg attccagacc agaatttatc ttcaaacgct tcggcttcgc
    25141 gcgccgctgc gttaagaata cgggtccgct ggataataat ggcccaagat gcgattgaaa
    25201 aaccaatcaa aatcaacatg ataagtttaa ccagaaggct agccttcagg aacaaatcaa
    25261 ggatattcat gtcagtcact gcttaaactc cgcgacaata gacttgggaa gcgcacgagg
    25321 cttcattttg agtgggtcaa cgcaaacaac cagaacctct gcttcattca gcaaagtatt
    25381 ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg atgttatttc
    25441 agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt
    25501 acgtaccaca aaggcaacgc gttcagccat cagcgcctgc tgactgaagt gatgatgacg
    25561 cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt acaccacacc
    25621 accggcatcg gtatcttcat agtagacgcg aaccggccat cgaaacagcg ttgtattcac
    25681 tttacatccc ggtaatgcaa caaaagttag agcttttaaa cgctgctact atacgcgcgc
    25741 gattgaggtt tgggaatgag ggcaagttaa gggagcgtaa aaatttatgg gcctggagcg
    25801 gcccataatt ttttaaagag aattctgatt taaaagaaga agaaaatcag cccgacaatg
    25861 agaacaatat cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga
    25921 aagcccacgc cgtgaatcac accagcacag acggcccaca tcagcaataa accatgccag
    25981 atttccagtt cactggtctt cgcggcaaaa cgagatgggt cccaaaacat acatcctgct
    26041 aacagaagcg ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat taccgcatac
    26101 aaagtcgcga taatcttact catcagatgt cttcttgacc ggctttgcct gattcgacgt
    26161 gttcaagcgc cagcgcggtg attaccccaa acgaacaggc aagaagcgtt cccagaatcc
    26221 atgcgaaata ccacattttt agctccttac ttagtacaga gagtgggtgt tacgttcaat
    26281 atcttctttg gtgatacgac cgaacatttt ccagtaacac caggcggtgt agagcagaat
    26341 gatcggtacc agaaccaccg caacccaggt catgacgtta agcgtcagct ggctggaagt
    26401 tgcatcccac attgtcagac ttgcgttcat catggtgctg gacggcatca caaacgggaa
    26461 cattgcgata ccggctgtca ggatgatgca ggccagcgtc agggaggaga acacaaacgc
    26521 ccacgcggct ttatccatac gtgcagtcag gatggtcagc agcggcagaa ccacacccag
    26581 tgccggaata gcccacagaa ttggcgtgtt gttgaagtta accagccatg cgccagcttc
    26641 acgaaccact tctttattca gtgggttaga ggctgcgtaa tggtccattg tcgatttcac
    26701 gacataacca tcgataccgt acatcaccca tacgccagcc agtgcgaaac agaccagtgt
    26761 caccagcgca gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg
    26821 catttgcaga taggttgcgc cctgagtaat gatcatccct acgctcacca cgcctgccag
    26881 caggccgaac gggttaagca actggaagaa gttaccggtg tagtacagac gcagatattc
    26941 atcaacgttg aacggtacgc cctgcaacag gttaccgaac gctacaccaa ttaccagcgg
    27001 cggaacgaag ctaccaatga agatgcccca gtcccacatg ttacgccagc gggtttcttc
    27061 aatcttggag cggtagtcaa aaccgaccgg acggaagaac aaagacgcca gcacgaggat
    27121 catcgccaca tagaagccgg agaacgcagc ggcatagacc atcggccagg cagcaaagag
    27181 tgcgccgccc gcggtgatca gccaaacctg gttaccgtcc cagtgtggtg caatggagtt
    27241 aatcataatt cgacgctcgg tgtcgttacg accgaggaaa cgggtgagca tgcccacccc
    27301 catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca gcagccacca
    27361 gataaaacgc aatacttcat aatcgatcat ttgacgactc ctgtcttagc gtgccggctg
    27421 agtagtcgtg gaagactgct caaagtgata gcgaccggtt ttcaggctgc ttgggccgag
    27481 gcgtgcaaat ttgaacatta agaacaattc tgccaccagg aacagggtat acaggccgca
    27541 aatcagcacc attgagaaga tgagatcgcc tgcggtcagt gacgagttcg ccacagctgt
    27601 cggcagcact tcaccgatag cccacggttg gcggccatat tcagccacga accagcccgc
    27661 ttctacagca atccacggca gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt
    27721 ctcgccaatg cggttgcgga tgacactcca gaaagagagc gcgatgattg ccagaagcag
    27781 gaagccacac gccaccatga tacggaacgc aaagtacagc ggcgctacac gcgggatgga
    27841 gtctttggtt gcctgttgaa tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg
    27901 tttcagcagc agaccgtaac cgaggtcttt cttcatgcta ttgaactggt cacgaaccgc
    27961 ctggtcggta gaaccagaac gcagttgttc gagcagagag tacgccttca tcccgttacg
    28021 aatgcgttct tcatgctgca ccatcagctc tttcaggccg ataaccgggg tatccacgga
    28081 acgcgttgca atgatgccca gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc
    28141 ttcctgatca ggaatgccga acagagtaaa ggcagcaggc gcaggttgcg tttcccactc
    28201 ggcttcaata gcagccagtt tggttttctg cacgtcgccc atttcgtagc cggattcatc
    28261 acccagaaca ataacagaca gaacagcagc cataccgaag ctggcagcga tagcaaagga
    28321 gcgtttagcg aaggcgaagt cacgaccttt cagcatatac catgcgctga taccgaggat
    28381 gaacatcgcg ccagtcacat aaccagacgc tacagtgtga acgaatttca cctgagcaac
    28441 cgggttaagc accagctcgg agaagctcac catctccata cgcatagttt caaagttgaa
    28501 atcggacgcg attgggtttt gcatccagcc gttcgcaacc agaatccaca gtgcggacag
    28561 gtttgaaccg agcgccacca gccaggtgac acacatatgc tgaactttac ccagacgatc
    28621 ccaaccgaag aagaacagac ctacaaaggt ggattcgagg aagaaggcca tcagaccttc
    28681 gattgccagc ggcgcaccga agatatcccc tacatagtgg gaatagtaag accagttagt
    28741 cccgaactgg aactccatgg tcagaccggt agccacaccc agagcgaagt tgataccaaa
    28801 caacttgccc cagaacttgg tcatatcttt ataaatctgt ttgccggaga ggacgtagac
    28861 cgtttccata atggccagca ggaacgccat accgagcgtc agtggcacaa aaaggaagtg
    28921 gtacatcgcg gtcaaggcaa actgtaagcg cgacagttcg actatatcta acatcatgac
    28981 tccttgctca tcgcatgaag actccgagag tgaaccccgt tagaaagggt cacacgcatg
    29041 ccccaataga aatttgtttg aacactttca tcataccatg tgaatgcaca caaaaacggc
    29101 gtaaagaaag gttgcaaaca tgttaataaa aactcaaatt gatcccacgt atatattacg
    29161 ccgcaaaatc cttacaataa acaggttttt gttgaagcaa atttgcattt ttcgacggtg
    29221 atcaatttat agctaaatta ccgcctttca gccaatttga tcgagaacaa tttatctctt
    29281 tttgatg
//
LOCUS       DAZFWP020000054        28691 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFWP020000054 DAZFWP020000000
VERSION     DAZFWP020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.054"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            28..1077
                     /locus_tag="ACHK0Z_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             28..1077
                     /locus_tag="ACHK0Z_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU4502175.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1151..2008
                     /gene="nfo"
                     /locus_tag="ACHK0Z_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             1151..2008
                     /gene="nfo"
                     /locus_tag="ACHK0Z_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU4502176.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2012..3100
                     /locus_tag="ACHK0Z_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             2012..3100
                     /locus_tag="ACHK0Z_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4502177.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3156..4406)
                     /locus_tag="ACHK0Z_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             complement(3156..4406)
                     /locus_tag="ACHK0Z_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4502178.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4506..5447)
                     /gene="rihB"
                     /locus_tag="ACHK0Z_RS10810"
                     /old_locus_tag="pgaptmp_002162"
     CDS             complement(4506..5447)
                     /gene="rihB"
                     /locus_tag="ACHK0Z_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU4502179.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5577..6275
                     /locus_tag="ACHK0Z_RS10815"
                     /old_locus_tag="pgaptmp_002163"
     CDS             5577..6275
                     /locus_tag="ACHK0Z_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU4502180.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6346..7596)
                     /locus_tag="ACHK0Z_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     CDS             complement(6346..7596)
                     /locus_tag="ACHK0Z_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4502181.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7690..8628)
                     /gene="psuG"
                     /locus_tag="ACHK0Z_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     CDS             complement(7690..8628)
                     /gene="psuG"
                     /locus_tag="ACHK0Z_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU4502182.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8616..9557)
                     /locus_tag="ACHK0Z_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             complement(8616..9557)
                     /locus_tag="ACHK0Z_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU4502183.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(9981..11672)
                     /gene="fruA"
                     /locus_tag="ACHK0Z_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     CDS             complement(9981..11672)
                     /gene="fruA"
                     /locus_tag="ACHK0Z_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU4502184.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(11689..12627)
                     /gene="fruK"
                     /locus_tag="ACHK0Z_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     CDS             complement(11689..12627)
                     /gene="fruK"
                     /locus_tag="ACHK0Z_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU4502185.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12627..13757)
                     /gene="fruB"
                     /locus_tag="ACHK0Z_RS10845"
                     /old_locus_tag="pgaptmp_002169"
     CDS             complement(12627..13757)
                     /gene="fruB"
                     /locus_tag="ACHK0Z_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU4502186.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSAPTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14125..15303
                     /gene="setB"
                     /locus_tag="ACHK0Z_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     CDS             14125..15303
                     /gene="setB"
                     /locus_tag="ACHK0Z_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU4502187.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMGTAAGLEIPTMLIAGYFAK
                     RLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLMP
                     GQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKDV
                     "
     gene            complement(15300..15554)
                     /locus_tag="ACHK0Z_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     CDS             complement(15300..15554)
                     /locus_tag="ACHK0Z_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4502188.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15709..16281
                     /gene="yeiP"
                     /locus_tag="ACHK0Z_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     CDS             15709..16281
                     /gene="yeiP"
                     /locus_tag="ACHK0Z_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU4502189.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16504..17970
                     /locus_tag="ACHK0Z_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /pseudo
     CDS             16504..17970
                     /locus_tag="ACHK0Z_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            18088..19074
                     /gene="yeiR"
                     /locus_tag="ACHK0Z_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             18088..19074
                     /gene="yeiR"
                     /locus_tag="ACHK0Z_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU4502190.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19113..19826
                     /gene="lpxT"
                     /locus_tag="ACHK0Z_RS10875"
                     /old_locus_tag="pgaptmp_002175"
     CDS             19113..19826
                     /gene="lpxT"
                     /locus_tag="ACHK0Z_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU4502191.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VLLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20238..20804
                     /gene="mepS"
                     /locus_tag="ACHK0Z_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             20238..20804
                     /gene="mepS"
                     /locus_tag="ACHK0Z_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU4502192.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            20985..22541
                     /locus_tag="ACHK0Z_RS10885"
                     /old_locus_tag="pgaptmp_002177"
     CDS             20985..22541
                     /locus_tag="ACHK0Z_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4502193.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22623..24437
                     /locus_tag="ACHK0Z_RS10890"
                     /old_locus_tag="pgaptmp_002178"
     CDS             22623..24437
                     /locus_tag="ACHK0Z_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU4502194.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24438..25532
                     /locus_tag="ACHK0Z_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             24438..25532
                     /locus_tag="ACHK0Z_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU4502195.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMIWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25532..26557
                     /locus_tag="ACHK0Z_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             25532..26557
                     /locus_tag="ACHK0Z_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4502196.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26559..28148
                     /gene="yejF"
                     /locus_tag="ACHK0Z_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             26559..28148
                     /gene="yejF"
                     /locus_tag="ACHK0Z_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU4502197.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28152..28496)
                     /locus_tag="ACHK0Z_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             complement(28152..28496)
                     /locus_tag="ACHK0Z_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU4502198.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         6994 a         7426 c         7001 g         7270 t
ORIGIN      
        1 ccttatgccc ggttaaagga gagggttatg acgaatatca ccttacagaa acaacatcgt
       61 acactgtggc attttattcc ggggttagcc ctgagtgcag ttatcaccgg ggtcgccctg
      121 tggggtggtt ccattcccgc ggttgcgggt gccgggttta gtgccctcac cctcgcaatc
      181 ttgttgggga tggttttagg caacaccatc tatccgcaca tctggaaaag ctgtgacggt
      241 ggtgtgctgt ttgccaagca atatttatta cgtctgggta tcattcttta tggcttccgt
      301 ctgacgttct cgcaaattgc cgatgtcggt atcagtggga tcatcattga cgtgctgacg
      361 ctgtccagta ccttcctgct ggcttgcttc ctggggcaga aagtgtttgg tctggataag
      421 cacaccagct ggttgatcgg tgccggtagc agtatctgtg gtgctgccgc ggtactggcg
      481 actgagccgg tagtgaaagc ggaagccagt aaagtaaccg tggctgttgc aaccgttgtt
      541 atcttcggga ccgtcgcgat tttcctctac ccggcgatat atccgctgat gtctcaatgg
      601 tttagtccgg aaactttcgg tatctacatt ggttctactg tgcacgaagt ggcgcaggtg
      661 gtggcggcag gtcatgccat cagcccggat gcggaaaacg cagcagttat ttccaaaatg
      721 ctgcgcgtga tgatgctggc tccgttcctc atcctgctgg cggcgcgtgt taaacagctg
      781 tctggggcga acagcggcga gaaaagcaaa atcactattc cgtggtttgc catattgttc
      841 atcgtcgttg ccatctttaa ctcgttccac ctgttaccgc agagcgtggt gaacatgctg
      901 gtaacgctgg ataccttcct gctggcaatg gcgatggcgg cgctgggtct gaccactcac
      961 gtcagcgcgc tgaaaaaagc cggggcgaaa cctctgctga tggcactggt actgtttgcc
     1021 tggctgattg ttggtggtgg tgcgattaac tatgtgattc aaagcgtcat cgcataaacc
     1081 actacatctt gctcctgtta acccgctatc attaccgttt tcctccagcg ggtttaacag
     1141 gagtcctcgc atgaaataca ttggagcgca cgttagtgct gctggcggtc tggcaaatgc
     1201 cgcaattcgc gccgccgaaa tcgacgcaac cgcgtttgcc ttgttcacca aaaaccaacg
     1261 tcagtggcgt gccgcaccgc tcacgacgca aaccatcgat gaattcaaag cggcctgtga
     1321 aaaatatcac tacacatcgg cgcaaattct tccccacgac agctatctga ttaacctcgg
     1381 acatccggtc actgaagctc tggaaaaatc gcgcgatgcc tttatagatg aaatgcagcg
     1441 ttgcgaacaa ctggggcttt ctttgctcaa cttccaccct ggcagccatc tgatgcagat
     1501 ttcagaagag gattgccttg cgcgtattgc cgaatccatc aacattgcgc tggataaaac
     1561 tcaaggtgta acagcggtga ttgaaaacac cgccggtcag ggcagtaact tagggtttaa
     1621 attcgaacat ctcgcggcga ttatcgacgg cgtggaagat aaatcccgtg tcggcgtctg
     1681 cattgatacc tgccatgctt tcgctgccgg gtatgatttg cgtactccag ccgaatgcga
     1741 gaaaacgttc gcggattttg cccgtattgt tggctttaag tatctgcgcg ggatgcacct
     1801 taacgatgcg aaaagcacct ttggcagccg cgttgaccgc catcatagcc tcggtgaagg
     1861 caatatcggt catgatgcgt tccgctggat catgcaggac gaccgtttcg acggcattcc
     1921 gctgatcctt gaaaccatca acccggatat ctgggcagaa gagatcgcct ggctgaaagc
     1981 gcaacaaact gaaaaagcgg tggcctgatc gatgaacgac agggaaaagc agattcttaa
     2041 aattttacgg cgtaacccgc tgattcaaca gaatgaaatc gccgatattc tgcaaatcag
     2101 ccgctcacgt gttgccgctc atattatgga tttgatgcgc aagggtttga ttaaaggcaa
     2161 aggatatatc cttaccgagc aggattactg tgtggttgtg ggtgcaatca atatggatat
     2221 tcgcggaatg gcggatatcc gttacccgca agcagcctct catccaggtt ctgttcactg
     2281 ttccgcaggc ggcgtcggac gtaatatcgc gcataacctg gctctgcttg gtcgggatgt
     2341 gcatttaata tcagctattg gcaacgattt ttacggcgag acattactgg aagaaacgcg
     2401 ccgcgcgggc gtgaacgttt caaactgcat tcgtctgcat ggacacagca ctgcaactta
     2461 tctcgctatc gctaataagc aggaagaaac tatcctggca atcaatgaca cacatattct
     2521 gcaacagcta acgcctcaac tgctcaatac ttcacgggac ttgattcgcc atgctggtgt
     2581 ggtgctggct gattgtaatc tcacacctga agccctggag tgggtcttta ccattgctga
     2641 tgaaattccg atgtttgtcg ataccgtttc cgaatttaaa gcgaacaaag taaaaaactg
     2701 gtacagccgg atccacactc tgaaaccaac acaaaatgaa ctggaaattt tgtgggggca
     2761 accgattaaa gacgacaacg atcgcatcag agctgtaaat tcgctgcatc aacaaggtgt
     2821 taaacgcatt tttgtttatc tgaaagatga atctgtattt tgcagcgata aggatggaga
     2881 acaatttttg ctgaccgcgc cagcgcatac gacagtagac agttttggtg ctgacgatgg
     2941 ttttatggcg ggcttggtat atagctttct tgaaggatac agtttccgcg acagcgcccg
     3001 ttttgcggta gcctgcgcgg caatttcgcg cgccagcggc agcttaaaca accctaccct
     3061 gtctgccgat aacgcgcttt cattagtgcc aatggtgtaa caatgttgcc ggattcgacg
     3121 cttgaccgca tcttatccgg ctcataaatt gcgcactacg ctaaaccaat aaagaatcct
     3181 gcaatagtag cactcatcag gttagaaagt gtcgccgccg ccagcgcgcg taaaccaagt
     3241 tgggcgattt ccggcgcacg gtgtggcgca accgcagaaa acgcccccac caccaccccg
     3301 atagaaccaa agttagcgaa accgcacaac gcgaaagaaa taatcgccac ggttttagca
     3361 tccagagtgc cacccgtttg cagatatggc gagaaattga gataagcgac aaattcattg
     3421 atcgccagct tctgcccaat caaactcccg gcaagatttg catcactcca gtcaaccccc
     3481 atcacccacg ccaatggggc caacaggtaa cctaaaatgg actccagcga ggcatgtgca
     3541 aaaccaaacc agccgccaac gccgccgata ataccattaa ttaacgcaat gatggcgaca
     3601 aatgccataa caactgtcgc tacaccggcg gcgattttca gcccggtcat tgcccctgtc
     3661 gcagcggctt caataatgct ttttggcggt gtttcggtga aagagaggtt attaaaggaa
     3721 acctgcgaag attccgtagc cgggcttaac aggcgggcaa acaagatccc gcctgggatc
     3781 gccattaacg atgccgccag caaatattcc acaggtacgc ccagggcggc gtaaccaatc
     3841 attgtcgaac cagcaatcga ggccatgcca ctacaaatcg ctgtaaataa ttcattgcga
     3901 ttcagacgat cgataaaggg tttgacgatt gccggaattt cgttttgccc gaggaaaatg
     3961 gtagtgaccg cgacgaatga ctcgatcttg ctgatattta acgctttctg gaagataccg
     4021 ccgagaatgc gaattaaaat ccccatcaca ccgatgtagt agagaatact caccagtgct
     4081 gtaacgaaga taattgccgg taatactctg aaaccaaaga taaatcctgc gccatcaaac
     4141 agcgtgtcca ttttcggccc gaccagcgaa ccgaagataa atgcgctacc cgcgtcgctg
     4201 tacgccatca ctttatgcac gccaaaagcg actttttcag cgacccaacg ccctggcggt
     4261 aaccaaagca taatgccgcc aatcacaacc tgtaacacta acgccgcgcc aacggtacgc
     4321 aggctgatct tcttcttgtt tactgacagc aaaaatgcaa ttgccagcaa taccaccatt
     4381 cccagaacac ttctcatgac atccatagtg attatctctt catgtcatat tgggcgacgg
     4441 ctttctgccc atcagaaata gccgggcctg catcttgcgc tatcggcccg gcttgttatc
     4501 aacgcttaat gggttttgat gtagccgcgc acgcactctt cgactaagcc ccagaaccag
     4561 tcggtatcaa tagtgatgcc gactttggta ttggcgggct tgccaagaac gcccagctcg
     4621 tcgcagacgg tgcgcccata gcaagggcca ctgttgacgt ccacttcgac gtacatctcc
     4681 tgggttttaa tgccatcagg gttaatcaga taaccgatgc aggtggcgtc gtgcaccggg
     4741 ccgccagcca ggccgtagtt ttcgaactgc gttttgagag tgaagttcat gatgtcgctg
     4801 aacagctctc cggcagggcc gcctgccctt tccatccgag caatcacgtc cggggtgcaa
     4861 acggtctggt tggtgagatc gaggcccatc atcactaatg gaacgccaga ggtgaacacc
     4921 acgcgggctg cttccgggtc agcaaagatg ttgaactcgg cagatggcgt gaagttgcct
     4981 gtaccgtaag cgccgcccat tagcacaatt tcacggattt tgggcaggat cgcgggttgc
     5041 atacgcattg ccaccgcgat atttgaaagc ggaccaaccg gcaccagagt gatatcgcca
     5101 tcgcttgcca tcagggtatc aatgatgtat ttcaccgcgt gggtactttc tgcctggcgg
     5161 gtcagcggct cgaataccgg gccatccagt ccggtttcac cgtgaatatt atcggcaacg
     5221 atttgttgac gcataatggg ttgcggcatc cccgcataaa ccggaacatt aatctccagt
     5281 ttctggcaaa cattcaggcc attaattaat gttttatcaa gcgtctgatt acccgctaca
     5341 atagtgatgc ctaataaatc tattgccgga tgtttcgccg ccatcattat agcaatagca
     5401 tcatcatgac ccggatcaca atccagaata atttttctct tttccattgt ttatttcctc
     5461 tgtttccagt tgcgttattt tttctacagc aaagaaaatg cgaccgggcc gatgattgaa
     5521 tcttaccaac cgcgtacgta tgctaaatat gagaaatctc atagcggata aacatcgtga
     5581 aagaaatcca caataatgat cttaagcagc aattgatgag tgaatccgcg tttaaggatt
     5641 gctttttaac ggatgtttca gccgatacgc ggctgtttca ttttttagcg cgtgactaca
     5701 ttgtgcagga agggcaacag ccgtcctggc tgttttacct gacgcgaggc cgcgccaggc
     5761 tttacgccac gctagctaat ggtcgcgtgt cgctgatcga tttctttgcc gccccctgtt
     5821 ttattggcga gattgagtta atcgacaaag accatgaacc gcgtgcggtg caggctattg
     5881 aagagtgttg gtgccttgcg ctccctatga aacattaccg cccgcggtta ttaaacgaca
     5941 cgctattttt acgaaaactc tgcgtcacct taagtcataa aaattatcgt aatattgttt
     6001 ctttaactca gaatcaatca tttccgttag tgaatcgcct ggcagcattt atattactct
     6061 cgcaggaagg tgatctttat cacgaaaagc atacgcaagc ggcagagtat ttaggcgttt
     6121 cttatcgaca tcttttatat gttctcgcgc aattcattca cgacggttta ttaactaaaa
     6181 gcaagaaagg gtatctcatt aaaaacagaa agcagttgtc aggactggcg ctggagatgg
     6241 acccggagaa taaattctcc gggatgatgc agtaaaaatt atttgcaata gcgcgattgc
     6301 cggatgcaac gcttaatacg ttttatccgg tctacaaatc gagcattacg ccagaccaat
     6361 aaagaacccg gcaatagtcg cactcatcag gttggaaagc gttgctgctg ccagcgcccg
     6421 taaaccaagc tgggcgattt ctggagcacg ttttggcgaa atagccgaaa atgcgccaac
     6481 gacaacaccg atagaaccaa agtttgcaaa cccacagagg gcgaacgaga taatggcaat
     6541 ggtcttgaca tccagcgtgc cgctcgtttg cagatagggc gagaaattga gataagcaac
     6601 aaactcatta atcgccagtt tctgcccaat caaacttccg gcaagatttg catcactcca
     6661 gtccaccccc ataatccatg ccaacggggc cagcacatag ccgaaaatgc tttccagaga
     6721 gacattggca aaaccaaacc agccgccaac tccaccaata atgccgttga tcagcgcaat
     6781 aattgccaca aatgccatta ccaccgtcgc cacaccggcg gcgattttca gcccggtcat
     6841 tgcaccgttc gccgcagctt caataatgct ttttggcggt gtttcgctaa acgacagatt
     6901 ttcaaacgta acttgcgaag gttccgtcgc cggactaaga atacgtgcaa acaggatacc
     6961 gccaggaata gccatcagcg aagcggctaa caaatagtca atcggcacgc ccattccggc
     7021 atagccaatc atcatcgaac ccgcaataga cgccatgccg ctacaaatag ccgtaaatag
     7081 ctcgttgcga ttcatgcgat caataaaagg tttaacgatc gccgggatct cattttgccc
     7141 gaggaaaata gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg
     7201 aaaaatactg ccaagaatac gaatcagcag ccccattacg ccaatgtagt acagcaagct
     7261 gataagcgcg gtaacgaaaa taatcgccgg gagtacgcga aaggcgaaga taaagcccgc
     7321 accgtcaaac agaacatcca ttttgggacc gaccagcgaa ccaaaaataa aggcacttcc
     7381 tgcgtcactg taggacatca ctttatggac gcctaatgcc gcctgctcta ctgcccattt
     7441 tcccggggga aaatagagca tgatgccacc aatggcaatt tgcagcagca gcgcagcgcc
     7501 aaccgtgcgc aagctgatgc tctttttatt taccgacaac acaaaggcaa ttgccagcaa
     7561 taccaccatc cccacaacac ttctcattat atccataatg attttccctt catgccagta
     7621 aacccggcgt cagcgccagg ttttggtatg tttgatgagt acgggcgacg gctttctgcc
     7681 cgccagaaat taacccacga gacgctgata ttctttggca atttcgctcg ccagaatggc
     7741 gttattaaag accagttgaa tattggattt caggctatca ccgcctgtta gctccgcaac
     7801 acgcgccagc aggaacggtg tactctcttt cccaataaca ccctgttctt cggcttcagc
     7861 aacggcttga tcaattgctg cattgatggt ttcttctggc atcgcaaact gttccgggat
     7921 cggattcgcc actaccaggc cgccgttcaa accggtttgc cacttcactg ccatcgcgcg
     7981 agcaatttcg cgggcactgt caagacgaat gctgacttca aatgggctgg ttcggcagaa
     8041 aaacgcaggc agcgcttttg tctgataacc aattaacggt acaccgaagg tttctaaata
     8101 ttctgttgtt aatcctaaat cgagaataga ttttgccccc gcacaaacaa cagtgacatt
     8161 ggtatttgcc agttcctgta aatcggcaga aatatcgaag gtatgttccg ccccgcgatg
     8221 cacaccacca attcccccgg tggcaaatac tttaattccg gcaagcgccg caataatcat
     8281 cgttgaagcc acagtggttg cgccattttt tccggcggca acaacaaaag gtaaatcgcg
     8341 acgactaact ttggtcacgt tatgtccttc acggcccagc aattcgattt cttctttact
     8401 taagcccact ttcatcacac cgccaataat ggcgatagtg gctggtactg cgccctgttt
     8461 acgaatagtt tcttctactt caattgcggt ttgggcattt tgtgggaatg gcattccgtg
     8521 agaaattatg gtcgattcca gcgcaacaac cggttttttg ttttttaaag cgtcctgcac
     8581 ttccggggaa atttgtaata attcagggga aatttttaat tcagacattc tgcgttctcc
     8641 actaacgata taacgttggc aatcgataaa tcggggttat tggtgtattc acaggagagc
     8701 gccattgacg aacatccctg tgcgaaacga acagattcgg caaacggcat tccgtctacc
     8761 caacacgaag caagtcccgc catcatggca tcgcccgctc cggtaacatt aataacattg
     8821 gttttgatcg gggcagacca gccactttca ccgctgatat cactgtaata aacgccatcg
     8881 ccgcccatgc tcaataccag tcggttcagg ccatgttgat ggaaccaggc agcaactttt
     8941 gccacatctc cacgccctga cagcgcaatc ccactcagtg tttccgcttc aaggcggttt
     9001 ggcttcagag tgtggatctg atttagacgg tcgcgcactt tgacacattt ccacgctgaa
     9061 accggatcga caaatacggg tacgttggca gcattatcca gaatccatgc cagtgcctct
     9121 tcactgatat tacagtccgc gacaatgacc tttgccctct gaataaattc acggtgctgt
     9181 gcgagatatt cagctgtaat agcgttgcta atattcatgt catttatagc aaccagcatt
     9241 tcaccggtat tatcgagtaa tgataaataa ctcgacgtat tttctcccgg cacaatcagg
     9301 catttatcga cataaacgcc agattgattg gtttgcgtta gcagcgattg accataaaaa
     9361 tcactaccta ctgcgctcag taaccaggct ttgttaccca gcaacgccag gttttgtgca
     9421 atatttcgcc ctactccacc aggtgtaaat tttattttac ctggatttga atccgcataa
     9481 tttaatgatt catgtgaata tccggcgaca tcaatattcg ccgaacctat aattacgaca
     9541 taatcctttt cgcgcatagc acgtcctctg gtaaaaaatt accaaccctg tcaatccaca
     9601 agaatataga tactgcaaag acagtttaaa catgtgttca tatttaaaca tgtgctcata
     9661 gtatgcatgt tttacgtaaa gtaaattgcc gccagagagg caaagcgggc agatttgagc
     9721 aggctcacaa aatgcgatta aatgccagaa ttacaaaagc gaggaatttg tgacgggatg
     9781 cacagaacag gaacactaaa aaagggcaga aaagaatctg cccttgagga aataacctta
     9841 gtgtttgatg aacgtttcag gtttctactg aaagataaaa ccatttaaat tcataaaatt
     9901 acattcacaa accggtcatt ctctttgcgc tcatccaatg atacagggca gtaaatttct
     9961 gccctgtaac acacctttta ttacgcggct ttcgctactg cgtccacttc cggacgtttc
    10021 aggaaggcat aggccaaacc cgccaccagc gtaccggcaa taattgctac caggtaaccc
    10081 aataccggcg taatagcgcc agggatcagc agaacaaaca gaccaccgtg cggtgccatc
    10141 agtttcgcac caatcgccat tgagattgcg ccagtcagcg ccccacccac gatacagcac
    10201 ggcagcacac gcatcggatc acgagcagca aacggaattg caccttccga aataaagcac
    10261 agtcccagta ccagagcggc tttgccacct tcctgctgcg ctttgtcgaa tttgcgacgc
    10321 gccaccattg ttgccaggcc cattgccagc ggtggcacca tacctgccgc cataatcgcc
    10381 gccatcgggc cataggtttg agtactcagc agacccacac cgaatgcgta cgctgcttta
    10441 tttaccggac cgcccatgtc agtacacatc atgccaccga ggatcgcccc cagcagaacc
    10501 gcattcgcag tccccatggt ctgcagccag tgagtcaacc cttcgagaat gccagcaacc
    10561 ggtttaccga tcaggtagat catcgccaga ccgaccacca gactggaaat tagcggaatg
    10621 atcaggatcg gtttcagcgc ctccatactc tgtggcagtt tcagttgcgt actgattaac
    10681 ttcgcaatgt aaccagccag gaagcccgca ataataccgc caatgaagcc agaaccggtg
    10741 ctgaccgcca gcataccgcc aatcagaccc ggcgtgagac ccggacgatc ggcaatggag
    10801 aaggcaatgt aacctgccag taccggcacc atcagcgcaa aggctgaacc accaccaatc
    10861 tgcatcagcg ccgctgccaa cgtacccggc tctttaaacg cttcgatacc aaaagcaaaa
    10921 gaaagcgcga tacacagacc acctgcaacg accatcggca gcatgtaaga gacgcccgtc
    10981 agcaagtgac ggtatgcgcc tgcactctct ttcttacctt cagaggtcgc cgtttgagct
    11041 ttgcccgccg gttcatacgg cgttgcttca gcaaccgctt tatccagttc ctgcgcggtt
    11101 ttcttcagcg ccagaccggt agaggtacga tacatcggtt taccagcaaa tttcgccaga
    11161 tccacttcga tatctgccgc cacaatcacc agatccgctg cggctacttc ctcgggagtg
    11221 attgcattac ccgcgccaac agaaccacgg gtttcaactt tcacccacca gccacgtttt
    11281 ttcgcttcgg tttcaatggc ttcagccgcc ataaaggtgt gtgctacgcc agtcgggcaa
    11341 gcagtcaccg caactacgcg tttcggaccg ctggcggcaa ccggtgctgt cgcagtgacc
    11401 ggcgcagtgt aaggtttcgc atggcctttg gcttcactca ggaacagctc agggtgcgca
    11461 actgcccggg aaatatcacc cagccagaca tttttaccgt tcagcgcgct gtcattcggg
    11521 atggaatcac cgagaacaat cgccatttct gcgtcgttcg gattgtcgat gatttccagt
    11581 tttgcttttc gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg tgcctgaccg
    11641 agattagcgt caataatcag cagcgttttc attatgcctc tcctgctgtc agttaaaagg
    11701 ttgtaagtcg acgcgcgcca tcattgcggc caactgcgga cgatcggtaa tacccacatt
    11761 gctttgactt accgccaggg ctgcaacagc agtcgccaga cgcagtgtgt gttcactgga
    11821 ttcacgcatc agcaagccat aaatcaggcc accaaccata gaatcccctg cgccaacggt
    11881 gcttacgaca tcgactgacg gtggtttggc gatccattcg ccggaggcat taacccaaag
    11941 cgcgccttcg gcacccagtg aaataacaac atgcgcaata ccttgttcac gtagcgcatg
    12001 tgcagcttca atcacatctt tcatttcagg cagtttacgg cctgcccaga tttccagctc
    12061 gcggcggtta ggtttcacca gccacggtgc cgctttcaaa cctgctacta acgcttcacg
    12121 gctactatca aagataatgc aaggacactg actacgcagg cgagtcatcc agtcggtgaa
    12181 cgcttccggg ctgacgcctg acggtaagct tccgctgaca cagaccatat cgaactgacc
    12241 gagccagctc agagaatcag tcacaaagcg ttcccagtcg gcgggggtga cttcaaaacc
    12301 cgagaagttg aagtcggtca cttcgccgtc tttttccgtc agcttaacgt taattcgggt
    12361 gcgcccctgt acaacctgga aacggttggc aatgcccagc tcgctgaaca gttgctgaaa
    12421 accatcctga ttgtctttac cgaggaagcc gccaacggtg acatcaattc ccaggtcttt
    12481 taatactttg gccacgttga tgcctttacc cgccgcatgc agaccggtgg ttttcaccag
    12541 gttcacttcg ccgcgttcaa tttccgggca gaaaccaaca aggtcataag ccggattaag
    12601 ggtgatagta gcaacacgtc tgctcattat gcgccctccc caagaccagc agcgatagcg
    12661 tcgccgattg ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa gcgtaggcga
    12721 tgacctttct taacgccaag tgccacaact ttcatcagac tacgtccgtt tgccggtttg
    12781 ccggtgccat caaggtttgt cacggtaata tcactgttaa attgtttaat ggtattgacc
    12841 agcatggtac ctggacgagc atgcaggccg tgttcattgc gcaccacaaa ctccgcgctt
    12901 aacacgtcgt cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt tgccgcatcc
    12961 gctttcagca agcggtcagc tttattgtcg agcaacaaat cagcgagacg cttaagaacc
    13021 gcaatgggct gatcgtcatt catcgccaca ctcaccagca tggctgccgt ttcgccgtcc
    13081 acatcaaaag catttgccgc acggcttacc gcaatcgcgc tacgcagatt gccttcggcg
    13141 ctatcgctca gccagatacc ctgtccgagg ttcagcggtt gttcattgat ggctttggtg
    13201 acgaaagtgg cgtcaactgc cctcgcctct ttcagacgcg cagcgttcag cgcctgaaga
    13261 gtcagcagat cgctggcgac gatatccagc gtcagcattt cgttgtcgag cttcagctgc
    13321 tcactctgct tttcgcccat cagtaatgcg cgaagttctt ctgctgttgg tgctgacttc
    13381 agttgttcag caacggaatc atcgctcagt acgtgggtca gctggcgtag caggcccaga
    13441 tgttcatccg agctggcagc aataccgatt gccacgtacg ctacctgacc gtcaccccag
    13501 gtgacgcctt ccgggaactg aaatacctga acgccggttt tcagcacctg atcgcgggtg
    13561 tcggtagtgc cgtgtggaat agcaatacca ttgccgagga acgttgaggt ttgctgttcg
    13621 cgcgccagca tgccattgac gtagccttct gctacattac cggcctgcac cagcgccgca
    13681 gcgacctggc gaatcgcctc ttctttgtct ccggcctttt cgcccggatg gatgtcctgt
    13741 acggataact ggaacatagt tctcctctct tgctgaattg aaacgattca gcctctatga
    13801 gaaaaaaagc gccaacctgg cttagggtta aagacaagat cgcgctgaaa cgtttcaaga
    13861 aagcataata cttctgtttc agcacgcaag gaaagccgta aagttatgag caaaagtttg
    13921 atgtgctgca caattttttg ccatttttcc taattgctga tgggaaaatc cgttatcagc
    13981 aatttcattt cagcagcgtc agcagccaat cagcaacagt gaaatggcat tttgattatt
    14041 cgtggcgctt ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa actccgcgtc
    14101 ttcctcttcc agtgatcgac cagcatgcat aactcccccg cagtctccag cgcgaaatcg
    14161 tttgacctga cctcgacggc gtttttaatc gttgcctttc tcaccggtat tgcgggcgct
    14221 ctgcaaaccc cgacactcag tatttttctt actgatgaag tacatgctcg tccggcgatg
    14281 gtgggattct tctttaccgg cagcgctgtc attgggattc tggtaagtca gtttctcgcc
    14341 gggcgctctg ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg cctgttaggc
    14401 gtgctggcct gcaccctttt tgcctggaat cgcaactact ttgttttgct attcgttggc
    14461 gtctttctta gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct tgcccgtgaa
    14521 catgccgaca aaaccggacg tgaggcggtg atgttcagct cttttttacg cgctcaggtt
    14581 tcactggcat gggtcattgg cccaccgctg gcttatgcct tagcgatggg tttcagcttt
    14641 acggtaatgt atctgagcgc agcggtagcg tttattgttt gcggtgtgat ggtgtggctg
    14701 tttttaccgt cgatgcaaaa agagcttccg ctggcgaccg gcacgatcga agcgccgcgc
    14761 cgtaaccgtc gcgatacgct gctgctgttt gtcatttgta cattgatgtg gggctcgaac
    14821 agcctgtaca tcatcaacat gccgctattt attatcaacg aactgcatct tcccgagaaa
    14881 ctggccggtg tgatggggac cgccgccggg ctggaaatcc cgacgatgtt gattgccgga
    14941 tatttcgcca aacgtctggg taagcgtttc ttaatgcgcg ttgctgccgt gggtggcgtc
    15001 tgtttttacg caggaatgct gatggcgcat tcacctgtca ttctgttggg cttgcagctg
    15061 ctaaatgcta tttttattgg cattctgggc ggcatcggga tgctctattt tcaggatctg
    15121 atgcccggtc aggcgggttc agccaccacg ctctatacca acacgtcgcg cgtgggctgg
    15181 atcatcgcag gatcagtggc gggcatcgtc gccgagatct ggaattatca cgctgtgttc
    15241 tggtttgcga tggtgatgat tatcgccact ctgttttgct tactgcggat taaagatgtt
    15301 taaggcgcgg tcagcatttc cagatcgata agccaggtca tggcctgctg acgcgagtta
    15361 ccgcacattt cagcgctggc ttgtaagcct gcacagactt tcggtcgcag tggcgaggtg
    15421 aatattttac agcgttgctg ttcatcaagc tgaatgcagg gcgtattggc gggcttgcca
    15481 tctggcatac cgggaatagg gctggaaatt gaaggggcag tgcaacacgc cccgcaaccc
    15541 ggacggcatt tcatacgttg ttctcttaag gttaaccacc ggattgatga gcgcgcacag
    15601 taccaccttt tttgcaccag caaaagtgcg aataccactt gcccgaaagg cccgtcgcga
    15661 gtactttgtc gcgatatttt tgacattttc gactacagga atttttcgat gccaagagcg
    15721 aacgaaatta aaaaaggtat ggtactgaat tacaacggca aactgctgct ggttaaggat
    15781 attgatattc agtcgcccac tgcccgcggt gccgctacgc tgtacaaaat gcgtttttct
    15841 gatgtccgta ccgggctgaa agtagaagag cgtttcaaag gtgatgatat cgttgacacc
    15901 gtgacgctga cccgccgtta cgttgacttc tcctatgtcg atggcaacga atatgtcttt
    15961 atggataaag aagactatac cccgtatacc ttcaccaaag atcagattga agaagagttg
    16021 ctgtttatgc cagagggcgg catgccggac atgcaggtgc tgacctggga cggtcaactg
    16081 ctggcgcttg agctgccgca gaccgttgat ctggaaatcg ttgaaaccgc accaggcatc
    16141 aaaggggcat ctgccagcgc ccgtaacaaa ccggcgacat tgagcactgg tctggtgatt
    16201 caggtaccag aatacttaag cccgggcgaa aaaattcgta tccatatcga agaacgccgt
    16261 tatatggggc gtgctgacta acttcagccg catgcagaaa agggatagct caggctgtcc
    16321 cttttttaat ttattatacc aatcttctat tttgcgcttc ctgaacacct tacgccctca
    16381 attttcactc gttgattgat cgccctcaca cttcatcgca ttaacaatcc agaccaattt
    16441 caattgctgt catataactt tacactgtca ttgttaattt atcgttacta agacgtgact
    16501 cctatgaaga caattgcctc cgttacgctc ccgcatcata tacacgctcc acgctacgat
    16561 cgccagcagt tgcaatcacg tatcgttcat tttggctttg gcgcctttca ccgcgctcat
    16621 caggcgttac tgaccgatcg tgtgctgaat gcccagggcg gcgactgggg gatctgtgaa
    16681 atcagcttgt tcagcggtga tcaactgatg agccagctcc gcgcacagaa ccatttatat
    16741 accgtgctgg aaaaaggtgc ggacggcaat caggtgataa ttgtcggtgc cgttcacgaa
    16801 tgccttaatg caaaactgga ttccttagcg gcaattattg agaaattttg cgagccacag
    16861 gtggcaattg tttccctgac gattaccgaa aaaggctatt gtattgaccc ggccaccggt
    16921 gcactcgaca ccagtaatcc gcggattatt cacgatctac aaacccctga agaacctcac
    16981 tccgcaccgg gtattctcgt cgaagcactg aaacgccgcc gtgagcgagg ccttacaccg
    17041 tttaccgtgc tctcctgcga caatattccc gacaatggcc atgtggtgaa aaacgcggtg
    17101 ctgggaatgg cagaaaaacg ttcgccagaa ctcgccgggt ggataaaata acacgtcagt
    17161 tttccgggaa ccatggtcga ccgcattgtt ccggctgcaa ccgacgaatc actggcagaa
    17221 atcagccagc atctgggggt aaatgatccc tgcgcgatta gctgcgaacc gtttatccag
    17281 tgggtggtgg aagataactt cgtcgctggg cgtcctgcct gggaagtcgc aggtgtacaa
    17341 atggtgaatg atgtcctgcc atgggaagag atgaaactgc gtatgcttaa tggcagccac
    17401 tcttttctcg cttatctggg ttacctttca ggattcgccc atatcagtga ttgcatgcag
    17461 gatcgcgcat ttcgccatgc cgccagaaca ttaatgctga atgagcaagc gccgacacta
    17521 caaattaaag atgtcgattt aacacaatat gcggacaagt taattgcacg ttttgctaat
    17581 ccggcgctga aacataagac ctggcaaatc gcaatggatg gtagccagaa attaccgcaa
    17641 cgtatgctgg caggtattcg catacatctg gggtgcgaaa cggactggtc gttgctggca
    17701 ttaggcgttg ctggctggat gcgttacgtc agcggcgttg atgatgccgg aaatgccatt
    17761 gatgttcgcg atccgcttag cgataaaatt cgcgaacttg ttgcgggcag cagcagtgaa
    17821 caacgcgtaa ccgccctgct ttccctgcgt gaagttttcg gtgatgatct gccagataac
    17881 ccgcattttg tgcaggccat cgaacaagcc tggcaacaaa tcgtacaatt cggcgcacat
    17941 caggcgctat taaacaccct caaaatttaa cgatttctgc ggttaaagcg gatgaagctc
    18001 accttcgtcc gctctcccct tctcttttct gcctttttta gccaggatta acgctcagtt
    18061 aacttaccag agtattgcgg agccgttgtg accaggacca acctcatcac cggttttctc
    18121 ggcagcggga aaaccacgtc gattcttcat ctgttagccc ataaagatcc caacgaaaaa
    18181 tgggcggtac tggttaatga atttggggaa gtcggaattg atggtgcttt gctcgccgat
    18241 agcggcgcat tgctgaaaga gatccccggc ggctgcatgt gctgcgttaa tggtttaccc
    18301 atgcaggtag ggttgaatac cttactgcgt cagggaaaac cagaccgctt gttgatagag
    18361 ccgaccgggc tgggccatcc gaaacagatc ctcgatctgt taaccgcacc agtctatgaa
    18421 ccgtggatag atctgcgcgc caccttgtgc attctcgatc cgcgcttgct gctggacgaa
    18481 aaaagcgcca gcaatgaaaa cttccgtgac cagctggctg ccgcagacat cattgtcgcc
    18541 aataaatccg accgtgcgac gcccgaaagt gagcaagcgc tacagcgttg gtggcagcaa
    18601 aatggtggcg atcgacaatt aattcacagt gagcatggga aagttgacgg tcatcttctg
    18661 gatttgccgc gtcgcaattt agccgagttg cccgccagcg ccgcgcattc tcatcagcat
    18721 gtcgtgaaaa aagggttagc agcgttaagc ctgccagagc atcaacgctg gcgtcgcagt
    18781 ctgaacagcg ggcaaggata tcaggcctgc ggctggatat tcgacgctga tacggtattc
    18841 gacaccattg gcattctgga atgggcgcga cttgcaccgg tggaacgcgt caaaggcgtg
    18901 ctgcgtattc ccgaagggct ggtacgaatc aaccgtcagg gcgatgacct gcacattgaa
    18961 acgcaaaacg ttgcgccacc ggacagccgt attgagctga tttccagcag cgaagctgac
    19021 tggaatgctt tgcagagcgc gctgttgaag cttcgtttag cgactaccgc gtaaggttgc
    19081 ctgcgttttt cagtaagata attagagaaa atatgattaa aaatttgccg caaatagtgt
    19141 tgttgaatat tgtcggcctc gcgctgtttc tttcctggta tattcccgtt aatcatggat
    19201 tctggttgcc gattgatgcg gatatttttt atttctttaa tcagaaactg gtcgaaagta
    19261 aggccttttt gtggctggtt gcattgacca acaatcgcgc tttcgacggt tgttctctgc
    19321 tggcgatggg aatgttgatg ctgagtttct ggctgaaaga aaacgcccct ggcagacgac
    19381 gtatcgtgat tatgggtctg gtcatgctat taactgcagt gctattaaac cagctgggtc
    19441 aggcattaat tcctgtaaaa cgggccagcc caacattgac ttttaccgat attaaccgcg
    19501 tcagcgaact gctctctgtt cccacgaaag atgcctcacg agatagcttt cccggcgatc
    19561 acggcatgat gctgcttatt ttttcggcat tcatgtggcg ttatttcggc aaagttgcag
    19621 gccttatcgc ccttattatt tttgtggttt ttgcatttcc cagagtaatg attggcgcac
    19681 actggtttac tgacatcatt gtcggttcga tgaccgtgat attgatcggt ttgccctggg
    19741 tgttgctgac gccattaagt gatcgattaa tcaccttttt tgacaaatca ctaccgggaa
    19801 aaaacaaaca tttccaaaac aaataactca cagtaattaa catcatcagg cttattttta
    19861 tagtgaggat aatcctgatg atgcgcaccg tgctttcatc catcgcgcgc aaaaatcatt
    19921 ctctaagtaa atgaatggat tgcatgcgtt tcactcaatc gtactttaat tgaccaatcc
    19981 cgcttattaa ctttctgtat cactttttct tataaaaaat catgtaaaac cgctcgccaa
    20041 gaccgcacca atcgggtaat ctcgaactcg ttttgcctcg gcggtagatt atcctcacag
    20101 catataattt tgtgcgttag tccacagatt tggccttaag gaattgtttc aacatgccca
    20161 ggtaattagt ctcgtgtcgc ttggcatttt tttataacga tatttgtcgt taaggacttc
    20221 aagggaaaac aaacaacatg gtcaaatctc aaccgatttt gagatatatc ttgcgcggga
    20281 tccccgcgat tgcagtagcg gttctgcttt ctgcatgtag tgcaaataac accgcaaaga
    20341 atatgcatcc tgagacacgt gcagtgggta gtgaaacatc atcactgcaa gcttctcagg
    20401 atgaatttga aaacctggtt cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg
    20461 ctgactggaa aggcgtacgt tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt
    20521 ctggtttcgt acagcgtaca ttccgtgagc aatttggctt agaacttccg cgttcgactt
    20581 acgaacagca ggaaatgggt aaatctgttt cccgcagtaa tttgcgtacg ggtgatttag
    20641 ttctgttccg tgccggttca acgggacgcc atgtcggtat ttatatcggc aacaatcagt
    20701 ttgtccatgc ttccaccagc agtggtgtta ttatttccag catgaatgaa ccgtactgga
    20761 agaagcgtta caacgaagca cgccgggttc tcagccgcag ctaataaacc gtttggatgc
    20821 aatcccttgg ctatcctaac gagttaactg aaagcactgc ttaggcagtg cttttttgtt
    20881 ttcattcatc agagaaaatg atgtttccgc gtcttgatcc aggctatagt ccggtcattg
    20941 ttatctttta aatgttgtcg taatttcagg aaattaacgg aatcatgttc atacgcgctc
    21001 ccaattctgg acgtaagctc ctgcttacct gcattgttgc aggcgtgatg attgcgatac
    21061 tggtgagttg ccttcagttt ttagtggcct ggcataagca cgaagtcaaa tacgacacac
    21121 tgattaccga cgtacaaaag tatctcgata cctattttgc cgacctgaaa tccactactg
    21181 accggctcca gccgctgacc ttagatacct gccagcaggc taaccccgaa ctgaccgccc
    21241 gcgcggcgtt tagcatgaat gtccgtacgt ttgtgctggt gaaagataaa aaaacattct
    21301 gttcatctgc gaccggtgag atggacattc cactcaatga attgattccg gcgctcgaca
    21361 ttaataaaaa tgtcgatatg gcgatcttac ccggcacgcc gatggtgccg aacaaacccg
    21421 caatcgtcat ctggtatcgc aaccctttgc tgaaaaatag cggcgtcttt gccgctctga
    21481 atctcaacct gacgccttca ctcttttata gttcacggca ggaagattac gatggcgtcg
    21541 ccctcattat tggcaatact gcgctatcta ccttttcttc acgtttgatg aacgttaacg
    21601 aattaaccga catgccagtc cgtgaaacta aaattgcggg cattcctctg accgttcggc
    21661 tttatgcaga tgactggaca tggaacgatg tgtggtacgc atttttactg ggcggcatga
    21721 gtggaactgt cgttggcctg ctctgctatt acctgatgag cgtacgtatg cgccccggca
    21781 gagaaatcat gaccgccatc aagcgcgaac aattttacgt ggcgtatcaa ccggtggtgg
    21841 atacacaagc tttgcgagta acgggcctgg aagtactgct acgctggcgg catcctgtcg
    21901 cgggagaaat tcccccggat gccttcatta actttgccga atcgcaaaag atgattgtgc
    21961 cgctgactca gcatctgttt gagttaattg cccgcgatgc cgcagaatta gaaaaagtgc
    22021 tgccggtagg cgtcaaattt ggcattaaca ttgcgccgga ccatctgcac agcgaaagct
    22081 ttaaagcaga tatccagaaa ctgctcactt ccctacccgc acaccatttc cagattgtgc
    22141 tggaaattac cgagcgcgat atgctgaaag agcaagaagc cacacaactc ttcgcctggc
    22201 tgcactcggt aggcgtagaa attgctattg atgacttcgg caccgggcac agcgcgctta
    22261 tctatcttga gcgttttacg ctcgattatc tgaaaattga ccgtggattt atcaacgcca
    22321 tcggtacgga aacgatcact tcacccgtac ttgacgcggt gctgacgctg gcgaaacgcc
    22381 tcaatatgct gacggttgct gaaggggtcg aaacgccgga acaagcgcga tggctaagcg
    22441 aacgcggcgt taatttcatg caaggctact ggattagtcg cccgttaccg ctggacgatt
    22501 ttgttcgctg gctgaagaaa ccgtatacgc cgcagtggta aggtgtgctt acgtccctta
    22561 ttattcatag tgaaagcatg ccggattgcg gctaatgatg agtaaaagga aatccgttgc
    22621 agatgattgt gcgcatactg ctgctgttta tcgctctgtt cacctttggt gcgcaggcgc
    22681 aggctatcaa ggaaagctat gcctttgccg tgctgggcga accccggtac gcgtttaatt
    22741 tcaaccattt tgattatgtg aaccccgccg cgccaaaagg tgggcagata acgttgtcag
    22801 ccctcggtac cttcgataat ttcaaccgct atgcactgcg cggcaacccg ggcgcacgca
    22861 ccgagcagct gtacgacacg ctatttacga cttccgatga cgaaccaggc agttattacc
    22921 cgctgattgc tgaaagcgca cgctatgctg acgattattc ctgggtggag gtcgctatta
    22981 atccgcgcgc ccgttttcat gatggttcgc ccattactgc ccgcgatgta gagtttactt
    23041 ttcaaaaatt tatgaccgaa ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg
    23101 tcaaagccat tgcgccgtta accgtgcgca ttgagttagc taaacccggc aaagaagata
    23161 tgttgagtct gttttcgctg ccggtatttc cagaaaagta ctggaaggat cacaaactta
    23221 gcgatccgct cgccacgcct ccgcttgcca gtggtccgta ccgaattacg tcctggaaaa
    23281 tggggcaaaa tattgtctat tcccgcgtga aagattactg ggcagcaaac ttaccggtaa
    23341 accgtggacg ctggaatttc gacaccattc gctacgatta ttacctcgat gataatgtcg
    23401 cctttgaagc gtttaaagca ggtgcctttg atttgcgtat ggaaaacgac gctaaaaact
    23461 gggccacgcg ttataccggt aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa
    23521 agaacgaatc agcccaggat acgcgctggc tggcgtttaa tatccaacgt ccggtattca
    23581 gcgatcgccg ggtccgggaa gcaatcaccc tcgcctttga ctttgaatgg atgaacaagg
    23641 cgttgtttta caatgcctgg agtcgcacga acagttattt tcagaatacc gaatacgcgg
    23701 ccagaaatta ccccgacgcc gcggagctgg tgcttctggc accaatgaaa aaagatctac
    23761 cgtcagaagt cttcacacaa atctaccagc cgccggtatc taaaggcgat ggctacgatc
    23821 gtgacaacct gttaaaagcc gacaaacttc tcaacgaagc gggctgggtg ctgaagggtc
    23881 agcaacgcgt taatgccaca acgggtcagc cactcagctt tgaattattg cttcccgcaa
    23941 gcagcaatag tcagtgggta ttgccgttcc agcacagcct gcaacggctg ggtatcaaca
    24001 tggacattcg caaggtggat aactcgcaaa tcaccaaccg catgcgcagt cgcgactatg
    24061 acatgatgcc gcgcgtatgg cgggcgatgc cgtggcccag ttccgattta cagatttcct
    24121 ggtcatcgga atatatcaat tccacttata atgcccccgg cgtgcaaagt ccggttatcg
    24181 actcgctgat caatcaaatt attgccgcgc agggaaataa agaaaaatta ctgccgttag
    24241 ggcgagcact ggatcgcgta ttaacgtgga attattacat gctgccaatg tggtacatgg
    24301 cggaagaccg tctcgcctgg tgggataaat tctcccaacc cgctgtacgc cctgtttaca
    24361 gcctgggtat cgatacctgg tggtatgacg ttaataaagc ggccaaactg ccgtcagcca
    24421 gacaacaggg agagtagatg ggcgcttatc tgattcgccg tctgttgctg gtgatcccaa
    24481 cgttatgggc gattatcact atcaactttt tcatcgtgca aattgcgcct ggcggtccgg
    24541 tcgaccaggc catcgccgcc attgagtttg gtaatgccgg agtattaccc ggcgcaggcg
    24601 gtgaaggtat tcgcgccagc catgcgcaaa cgggcgtcgg caatatcagc gacagtaatt
    24661 accgtggcgg acgcggatta gatccagaag tgatcgccga gatcactcat cgctacggtt
    24721 tcgataagcc gatccacgaa cgttacttca aaatgatctg ggactacatc cgctttgatt
    24781 ttggtgatag cctttttcgc agcgcctcgg tgctgacgct gattaaagac agtctgccgg
    24841 tttccatcac cctcggattg tggagcacgc tgattatcta tctggtgtcg attccgttag
    24901 gcattcgcaa agctgtttat aatgggagcc gctttgacgt ctggagtagc gcatttatca
    24961 tcatcggcta cgccattccg gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg
    25021 gcggcagcta tttcgacctg ttccctctac gcggcctggt ttccgctaac tttgattcgc
    25081 tgccgtggta tcagaaaatc accgattatc tgtggcatat cacgctgccg gtgctggcga
    25141 cagtgattgg tggctttgcg gcgctgacca tgctgaccaa aaactcattc cttgatgaag
    25201 tgcgcaagca atacgtggtg accgcgcgtg cgaaaggggt aagtgaaaaa aatattctct
    25261 ggaaacatgt gttccgcaac gccatgctgc tggtgattgc cggttttccg gcgacgttta
    25321 tcagcatgtt ttttaccggc tcgctgctga ttgaagtgat gttttcactc aatggccttg
    25381 gcttactggg ctacgaagcg accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt
    25441 atattttcac cctgattggc ctgctgctga atattgtcag tgatatcagc tatacgctgg
    25501 tcgatccgcg tatagatttt gagggacgtt aatgtcgcga ctcagccccg tcaatcaggc
    25561 ccgttgggcg cgttttcgcc ataaccgtcg cggctactgg tcgttatgga ttttcctcgt
    25621 tttgtttggt ttgagtttgt gttctgaact tatcgccaac gataaaccgt tgctggtacg
    25681 ttatgacggc agttggtatt tcccgttgtt gaaaaactac agcgaaagcg attttggcgg
    25741 cccgctggca agtcaggctg attatcagga cccgtggctg aaacaacggc tggaaaataa
    25801 cggctgggta ctgtgggcac cgattcgctt tggtgctacc agtatcaact ttgctaccga
    25861 taagcccttc ccttctcccc cctcccggca aaactggctg ggaacggatg ccaacggcgg
    25921 cgatgtgctg gcacgcattc tctatggcac gcggatctcg gttctgtttg gcctgatgct
    25981 gactctctgt tccagcgtga tgggcgtgct ggcgggggcg ctacaaggct attacggcgg
    26041 taaagtcgat ctctggggac aacgctttat tgaagtatgg tcggggatgc cgacgctgtt
    26101 tttgattatt ttactttcca gcgtcgtaca gcctaacttc tggtggctgc tggcaattac
    26161 tgtcttgttt ggctggatga gtctggtcgg cgtggtgcgg gcggagtttt tacgtactcg
    26221 taatttcgac tacattcgtg cggcacaggc gcttggcgtc agcgatcgca gtatcatcct
    26281 gcgtcatatg ttgcctaatg ccatggtcgc gaccctcacc tttttaccgt ttattttatg
    26341 tagttcgata accaccctga cctcgctcga tttcctcggc ttcggtctac cgctcggttc
    26401 accgtcactc ggtgaactgc tgttacaagg gaaaaataac cttcaggccc cgtggcttgg
    26461 gatcaccgcc ttcttgtcgg tggcgatatt attgtctttg ctgatcttta ttggtgaagc
    26521 cgtccgcgac gcatttgatc ctaataaggc ggtgtagcat gacgcaaact ctgttagcga
    26581 ttgaaaattt gtcggtgggt tttcgccatc agcaaaccgt acgtacagta gtcaatgatg
    26641 tttcactaca gattgaggct ggcgaaacgc tggcgctggt gggtgagtca ggttcaggca
    26701 aaagcgttac cgcgctgtca attttacgcc tgctcccttc cccgccggtt gaatatctct
    26761 ccggcgatat tcgttttcat ggcgaatcgc tgcttcatgt cagcgatcaa acgttgcgcg
    26821 gtgtacgcgg taataagatc gccatgattt ttcaggaacc gatggtgtcg ttaaatccat
    26881 tgcataccct ggaaaaacag ctttatgaag tgctttcact ccaccgcggg atgcgtcggg
    26941 aagcggctcg tggcgaaatt cttaactgcc ttgatcgcgt tggtatccgc caggcggcaa
    27001 aacggctgac agattatccg catcagctct ccggcggcga acgccagcgg gtgatgattg
    27061 cgatggcgct gttaacgcga ccggaattat taattgccga tgaaccgacc accgcactgg
    27121 acgtctctgt ccaggcgcag attttacagc tgttgcgcga actgcaaggc gagctgaata
    27181 tgggcatgct gtttattact cataacctca gcattgtcag aaaactggcc caccgcgtgg
    27241 cggtaatgca aaacggtcgc tgtgtcgagc aaaattacgc cgctacgcta tttgcatcac
    27301 ccactcatcc ttacacacaa aagctactca acagtgaacc gtcaggcgat ccagtgccgt
    27361 tgccagaacc tgcctcaacg ttgctggatg ttgaacagct tcaggttgcc ttccccattc
    27421 gcaaagggat tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac atcagtttta
    27481 cgctacgagc gggtgaaaca ctgggtttag tgggcgagtc cggttccggg aaaagtacga
    27541 cgggactggc gctgctgcga ctgattaatt ctcagggcag catcatcttt gacggtcagc
    27601 cactgcaaaa tttaaatcgc cgccagctgt tacctattcg tcatcgcatt caggtggtat
    27661 ttcaggatcc aaactcctcg ctcaacccac gactcaacgt tttgcagatt attgaggaag
    27721 gcttacgggt tcaccagccg acgctttctg ccgcacaacg cgaacaacaa gtgatagccg
    27781 tgatgcatga agtgggatta gatcctgaaa cacgccaccg ttatccggcg gagttctctg
    27841 gtggtcagcg acaacgtatt gcgattgcca gggcattaat tcttaagccc tcgctgatca
    27901 tacttgatga accgacatca tcactcgaca aaacggtaca ggcgcaaata ttgacgctat
    27961 tgaaatcatt gcaacaaaag catcaactgg cctatttgtt tatcagccac gatttgcacg
    28021 ttgtccgcgc gttatgtcat caggttatcg tactgcgaca aggggaagta gtggaacaag
    28081 gaccgtgcgc gcgcgtgttt gccacaccgc agcaggagta tacgcgtcag ctactggcgt
    28141 tgagctgacg cttaaaaagg attgtagttt gaaaagggtt cggcaattgc cacaccaaaa
    28201 tttttcaatc ggcatgttgc agcaaactcg tcctggcgat ttacaaacag gcacggctca
    28261 ccttcacact ccagcaccga acatggaact tcgatgtcgc ttaacgcctg gcttagttta
    28321 tacatcaccg accatgcatt atcaccatcc gggctaagca atttaagcgc caccaggctg
    28381 ttgtcaccgc acggtccatt ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca
    28441 gaccagcgat agttctgcgg cagtcgatgg acgattgaga gttgtaatga agtcacttat
    28501 tttccccgga agtacattca cttcacaatt tgtttacatc aattttaaca catcatcaac
    28561 aatccgtctt tatacagatc gtaaagattg atgcctcgcc tgcgtggcct ctatggctct
    28621 gatttaagta atatctgcgg gcgtggtcac gtttgcgggg ctatatgtac ccgtttctgc
    28681 gatctaactc a
//
LOCUS       DAZFWP020000055        28260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFWP020000055 DAZFWP020000000
VERSION     DAZFWP020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.055"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(60..1457)
                     /gene="ccp"
                     /locus_tag="ACHK0Z_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             complement(60..1457)
                     /gene="ccp"
                     /locus_tag="ACHK0Z_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU4502199.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1862..3511
                     /locus_tag="ACHK0Z_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             1862..3511
                     /locus_tag="ACHK0Z_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4502200.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3562..4164)
                     /locus_tag="ACHK0Z_RS10925"
                     /old_locus_tag="pgaptmp_002185"
     CDS             complement(3562..4164)
                     /locus_tag="ACHK0Z_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4502201.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EISRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4684..5583
                     /locus_tag="ACHK0Z_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             4684..5583
                     /locus_tag="ACHK0Z_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4502202.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5632..6645
                     /gene="yhjD"
                     /locus_tag="ACHK0Z_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             5632..6645
                     /gene="yhjD"
                     /locus_tag="ACHK0Z_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU4502203.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7056..8378
                     /locus_tag="ACHK0Z_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             7056..8378
                     /locus_tag="ACHK0Z_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502204.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8612..10672)
                     /locus_tag="ACHK0Z_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             complement(8612..10672)
                     /locus_tag="ACHK0Z_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4502205.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10742..11509)
                     /gene="pdeH"
                     /locus_tag="ACHK0Z_RS10950"
                     /old_locus_tag="pgaptmp_002190"
     CDS             complement(10742..11509)
                     /gene="pdeH"
                     /locus_tag="ACHK0Z_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU4502206.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11741..12670
                     /gene="kdgK"
                     /locus_tag="ACHK0Z_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             11741..12670
                     /gene="kdgK"
                     /locus_tag="ACHK0Z_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU4502207.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12766..14262)
                     /locus_tag="ACHK0Z_RS10960"
                     /old_locus_tag="pgaptmp_002192"
     CDS             complement(12766..14262)
                     /locus_tag="ACHK0Z_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4502208.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14483..15769)
                     /gene="dctA"
                     /locus_tag="ACHK0Z_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             complement(14483..15769)
                     /gene="dctA"
                     /locus_tag="ACHK0Z_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU4502209.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15952..17940)
                     /gene="hmsP"
                     /locus_tag="ACHK0Z_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             complement(15952..17940)
                     /gene="hmsP"
                     /locus_tag="ACHK0Z_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU4502210.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18022..21495)
                     /gene="bcsC"
                     /locus_tag="ACHK0Z_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             complement(18022..21495)
                     /gene="bcsC"
                     /locus_tag="ACHK0Z_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU4502211.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21477..22583)
                     /gene="bcsZ"
                     /locus_tag="ACHK0Z_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             complement(21477..22583)
                     /gene="bcsZ"
                     /locus_tag="ACHK0Z_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU4502212.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22590..24929)
                     /gene="bcsB"
                     /locus_tag="ACHK0Z_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             complement(22590..24929)
                     /gene="bcsB"
                     /locus_tag="ACHK0Z_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU4502213.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMLGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24940..27558)
                     /gene="bcsA"
                     /locus_tag="ACHK0Z_RS10990"
                     /old_locus_tag="pgaptmp_002198"
     CDS             complement(24940..27558)
                     /gene="bcsA"
                     /locus_tag="ACHK0Z_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU4502214.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQIPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27555..>28260)
                     /gene="bcsQ"
                     /locus_tag="ACHK0Z_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     CDS             complement(27555..>28260)
                     /gene="bcsQ"
                     /locus_tag="ACHK0Z_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGU4502215.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINNFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSAS"
BASE COUNT         6619 a         7832 c         7165 g         6644 t
ORIGIN      
        1 cagactttat tatcgtaata aaaatcgccc aaacattcct gttcgggcga tcaaattaat
       61 tattgtttat cctgcatata cggcgtgtac accccgttca gactgtgcag gaaagctacg
      121 atatcatcca catcctcctg cggcagctct ttgcctacct gatagcgcag catcagtttc
      181 accgccccgt ccagcgtcgg cacgtcaccg cgatggaagt acggtgccgt taaagcaacg
      241 ttacgtaaac cgggtacttt ctgacgcaat ttatcacgct cttctttagt cacattcata
      301 cgaccaatat ccgccgccgt aatttcccca aagttaaagt cttttttcag ccccaacggt
      361 tcaaaggaac gtccgccgag aataatacca ccatgacaag ttgcacattt attatcttta
      421 aataattgat agcctttttt ctgttgcgcc gtcagcgcat tttcatctcc acgcaaccat
      481 ttatcaaatg gggaatccgg cgtaattaat gttttctcaa attcagcaat ggcatcagta
      541 atattttcgc cactgaaacc ttgcggataa acttcgagga actgcgcttt aagctgcgga
      601 tctttttcca gcttagcaat aatttcgtcc caggatttcg acgccatttc aatcgggttc
      661 aacggcggtc cacctgcctg atcctgcaat gttgccgcac gaccatccca gaactgctca
      721 acgttaaata ctgagttaaa taccgtcggc gcgttaatcg gcccaactgc gccaccaaca
      781 ccaatcgatg tttttctgcc atcgacgccc cccgcattca acgcatggca atgagcgcat
      841 gaaatggtgc tatcagccga taaacgggga tcgtgataca gcgcaaagcc caacgccact
      901 ttttgcgcat cggtaggcag tttttgcggg atgggctgca ccggttcatt gcgatgctcc
      961 ggagcagtat cattgctggc gtaatattct gcgcgctgtt ttgcaatcca ggccagtatt
     1021 tccgcccgct cttcatcact caccttaccc gcccagtgta gcgcggtata acgcgttggt
     1081 ggcatagttt catactgcat cacccattca atcttattca gatcgctttg cgaaacgggt
     1141 ttatcagcca gcagtgccgc acgcacggcc tcgaggttaa aagatttata tccaagctta
     1201 atgtcgtaat ccatcaactg tttcgcgcca ggaatataat aataggcggg taattctgcc
     1261 gaaggtgtgt ggcaatagtc gcatcctttt tcgcggagaa agcctaaaac cttattattt
     1321 tcactgacag cagatgcctg aacatcggcc tgtttactgc gtttattatc gtggtaccac
     1381 acataaccag ataaccctaa atagcaaatc gcgacgccag ccaggccgat cgcggtaata
     1441 cgtgagacca ttttcattat tcttccctca cggtggtgag ttattgtgac ccagaaaatt
     1501 ttccagaatg ctgcaaacgt ctctgcgaga caaagccatc ataaaaaaca gggagatacg
     1561 ctttttgata ggaatcaatc aattacatga atataaacta tcaataagat agcctgtgcc
     1621 tattacccag atcaggaatg gcatatcatg attgcggcga gagcaggatt gaatgttctg
     1681 aaaatgaaaa acaacacgaa atccgtattt ttataaattc catggatatt agtgcgttat
     1741 attttttgac aggttaataa acgtgatcca ccgcacgctt tgtcgcccac caggcggagc
     1801 gaatgactac ccttaaagaa aagcccgata attagcgacg aatttcggag gttggatcct
     1861 tatgctcaat cagaaaattc aaaaccctaa tccagacgaa ctgatgatcg aagtcgatct
     1921 ctgctatgag ctggacccgt atgaattaaa actggatgag atgatcgagg cagaaccgga
     1981 acccgagatg attgaagggc tgcccgcctc tgatgcgctg acgcctgccg atcgctatct
     2041 cgaactgttc gagcatgttc agtcggcgaa aattttcccc gacagtaaaa cctttcccga
     2101 ctgcgcaccc aaaatggacc cgctggatat tttaatccgc taccgtaaag tgcgccgtca
     2161 tcgtgatttt gacttgcgca agtttgttga aaatcacttc tggctgccgg aggtctactc
     2221 cagcgagtat gtatcggacc cgcaaaattc cctgaaagag catatcgacc agctgtggcc
     2281 ggtgctaacc cgcgaaccac aggatcacat tccgtggtct tctctgctgg cgctaccgca
     2341 gtcatatatt gtcccgggcg gccgttttag cgaaacctac tattgggatt cctatttcac
     2401 catgctgggg ctggcggaaa gtggtcggga agatttgctg aaatgcatgg ccgataactt
     2461 cgcctggatg atcgaaaact atggtcacat ccccaacggc aaccgcacct attatttgag
     2521 ccgatcgcaa ccaccggttt ttgcgctgat ggtggagttg tttgaagaag atggtgtacg
     2581 cggtgcgcgc cgctatctcg accaccttaa aatggaatat gccttctgga tggacggtgc
     2641 agaatcgttg atccctaatc aggcctatcg ccatgttgtg cggatgccgg acggatcgct
     2701 gctcaaccgt tattgggacg atcgcgacac gccgcgtgac gaatcctggc ttgaggacgt
     2761 tgaaaccgcg aaacattctg gtcgcccgcc caacgaggtg taccgcgatt tacgcgcggg
     2821 agcggcctca ggttgggatt actcttcccg ttggctgcgt gatactggtc gtctggcgag
     2881 cattcgtacc acccagttca tccccatcga tctgaatgcc ttcctgttta aactggagag
     2941 cgccatcgcc aacatctcgg cgctgaaagg cgagaaagag acagaagcgc tgttccgcca
     3001 gaaggccagt gcccgtcgcg atgcggtaaa ccgttacctc tgggatgatg aaaacggcat
     3061 ctaccgcgat tacgactggc gacgcgaaca actggcgctg ttttccgctg ccgccattgt
     3121 gccgctctat gtcggcatgg cgaaccatga acaggccgat cgtctggcaa acgccgtacg
     3181 cagccggtta ctgacacctg gcgggattct ggcaagcgag tacgaaaccg gtgaacagtg
     3241 ggataaaccc aatggctggg caccgttaca atggatggca attcagggat ttaaaatgta
     3301 tggcgatgac cttctgggtg atgaaatcgc gcgcagctgg ctgaaaacgg tgaatcagtt
     3361 ctatctggaa cagcacaaaa tgatcgagaa ataccatatt gccgatggtg ttccccgcga
     3421 aggcggcggt ggcgagtatc cgttgcagga tgggtttggc tggactaacg gtgtggtacg
     3481 ccgtttaatt ggtttgtacg gcgaaccata atatttttac agccagccgc taacttcctg
     3541 ctggctgtaa aattatcctc ttcaggagga gatatttaac atcattgccg cctgggtgcg
     3601 atttttcact tccagacggc gatacaggga ttccagatgc gcttttaccg ttccggtact
     3661 gatattcagc gctctgctga tctccttatt tgattcgccc gccgctaaca tggttaaaat
     3721 ctcccgctgg cgggcgctta acgatttgag atctttaatg tccttttccg gcgtcgtccg
     3781 ccagtctcca ggcagaaaca tcatccccat cgccgcacta tttaccgcca acgcaaatgt
     3841 ctcgacggtt gaatcacgag gcacaatggc cagcacatta aaatgaataa cttcctgtaa
     3901 ccaccgttta ttgcaatccg tcgccgtgat taacacctta acctcaggaa actgcaccac
     3961 ggttttttgc agcaaccagt agcaaaactc accatcctga tcgccatcga gcataactaa
     4021 ggcttcaggg taactttcca gcttttgcca taactcgtct gcctgactgg ccccctgaat
     4081 actcactcct ggaatacgct gctgtaaact gattttcatt ccatgaataa atattgactg
     4141 cctgtcaaac atgactattt gcataactga atctccacct gaatacgtta aaaagactta
     4201 agtagtggaa gggtattacc cgcgagaaaa aataagaatt cgccatttgg cggtggccat
     4261 tctacagaga tgacgcgtag aaaatagtta ccgatataaa tagttacagc taaacgcctg
     4321 aaattacatg tcgagggcac tatttaaaac aattttgagg atttccttat attggtggtt
     4381 agtacgcatg caattaaaaa tgaaactccg cgaccacaag caaaaataac aaacggcaat
     4441 gagacaaaaa taagcacaaa tagccaacac gtcctctatt cactttaaag ggaatcgctg
     4501 aaaaatacgc tctgtttagg gggattcacc tttctcagaa agctattccg cccttttcct
     4561 gctgagaaat cgccacattc gtcatgacaa cattgtgaaa cccggcatta gatgttagaa
     4621 aaaacaataa acaatgcgat atgcgcgtta ctgtttcgct gtgaacaaca atcggtcaaa
     4681 gaaatggata aaattcacgc aatgcagttg ttcatcaaag tcgcggagct ggaaagtttt
     4741 tcccgcgcgg cggatttctt tgctttgcca aaaggaagtg tttcgcgcca gatacaggca
     4801 ctggaacatc aacttggcac ccagcttctc cagcgcacca cgcgacgggt caaactcacg
     4861 ccagaaggca tgacctatta tcaacgagca aaagatgtgt tgagtaatct cagcgaactg
     4921 gacggtctgt ttcaacagga tgccaccagt atcagcggta aattacgcat cgacatcccg
     4981 ccaggaatcg cgaaaagcct gttactgccg cgcctgtcgg aatttctcta tctgcatccg
     5041 ggaattgagc tggaactgag tagccatgac cgtccggtag atattcttca tgatggtttt
     5101 gattgcgtga tacgcactgg cgcgttaccg gaagatggcg ttatcgcccg tcccctcggc
     5161 aaactgacca tggtcaactg tgccagtccg cactatctga cgcgctttgg ttatccgcaa
     5221 agccccgatg atctgacttc acacgcaata gtgcgttata caccgcacct gggtgtacat
     5281 ccgttaggtt ttgaggttgc cagcgttaat ggcgtccagt ggtttaagtc tggcggcatg
     5341 ttgacggtaa acagtagcga aaactatctc gccgccggtc ttgccggtct ggggattatt
     5401 cagatcccgc gcattgccgt gcgcgaagcc ctgcgtgccg ggcggcttat tgaagtgttg
     5461 ccaggctacc gtgccgagcc gctctccctt tcgctggttt acccgcagcg tcgggagctt
     5521 tcccggcgtg taaacctgtt tatgcagtgg ctggctggcg taatgaaaga gcacctggac
     5581 tgaccgacta tactttttaa gaatgaccac gacagaagga caaaagagcg gatgacgcag
     5641 gaaaacgaga tcaaacgtcc cacccaggat ctggagcacg agccgattaa gcagttggat
     5701 aatagcgaga aaggcggcaa agttagccag gcgctggaaa ccgtcaccac cactgccgaa
     5761 aaagtccaac gccaaccggt tattgcgcac ctgattcgcg cgacagaacg ctttaacgat
     5821 cggctgggta accagtttgg tgcggctatc acctatttct catttttatc gatgatcccg
     5881 attttgatgg tgtcgtttgc cgcagggggc tttgtgctgg cctcccatcc gatgttgcta
     5941 caggatatct tcgacaaaat tctgcaaaac atcagcgatc cgacgctagc cgccacgttg
     6001 aaaaacacca tcaacaccgc cgttcagcag cgtacgactg tagggcttgt cggcctggcg
     6061 gtggcgcttt attctggcat caactggatg ggtaacctgc gtgaagcgat tcgtgcccag
     6121 tcgcgcgatg tctgggaacg ctcgccgcaa gatcaggaga agttctgggt aaaatatctg
     6181 cgtgatttta tttcactgat tggtttgtta attgcgctga ttgtgacgct gtcgatcacc
     6241 tcggttgccg gttcggcgca gcaaatgatt attagcgccc tgcacctgaa cagcattgag
     6301 tggctgaaac cgacgtggcg gttgattggc ctggcgattt ccatcttcgc caactatctg
     6361 cttttcttct ggatcttctg gcggttgccg cgccaccgtc cgcgcaaaaa agccctgatt
     6421 cgcggaacat ttctggctgc tattggtttt gaagtgatta aaatcgtgat gacctacacc
     6481 ttgccatcgc tgatgaaatc cccctctggc gcagcatttg gttccgtgct ggggctgatg
     6541 gcatttttct acttcttcgc ccgtttgacg ctgttttgcg cggcgtggat tgccaccgcc
     6601 gaatataaag acgacccgcg aatgccaggg aaaacgcagc cttaaaatag gccggatgcg
     6661 gcgtaaacgc cttatccggc atacgatttt attcggcctg gctccccgta ggccggataa
     6721 gatgcgccag catcgcatcc ggctataatg cgcacataac ctcttgaaac tcttccccag
     6781 agcctcttca gccatctatt tgggagcaaa caatttcatt ccaactcata accccagcat
     6841 ataaatccag ttggtaactt ttatttaacc tgaaaccagt tttatccact atttataaaa
     6901 ttatgtgaag catttcatag aagaaaaatc actggcctaa acattatccc ctttttgcct
     6961 ggtttttgac catttccgcg atttgttaca cattgaaata tcacttttgc tgtgcgtaat
     7021 atggctattc gttagccaaa aaataagaaa agattatgca agcaacagcc acaacactcg
     7081 accacgagca agaatacacg ccgatcaact cgcgtaataa agtccttgtc gcctctctca
     7141 ttggcacggc cattgagttc ttcgactttt acatttacgc cactgcggcc gttattgtgt
     7201 ttccgcatat cttcttcccg cagggcgatc caacggcagc aacgctacag tcgctcgcca
     7261 ccttcgccat cgccttcgtc gcgcgcccca ttggctctgc cgtttttggt cattttggcg
     7321 atcgcgttgg gcgtaaagcg acgctggtcg cctcgttgct aacgatgggg atttcgaccg
     7381 tggtgattgg tctgctgccg ggctatgcca cgattggtat tttcgccccg ctgctgctgg
     7441 cgctggctcg atttggtcag ggtctgggct taggcggtga atggggcggc gcggcgctgc
     7501 tggcgactga aaacgcccca ccgcgcaaac gtgcactgta tggctccttc ccacagctgg
     7561 gcgcaccgat tggcttcttc tttgccaacg gcactttctt gctgctttcc tggctactga
     7621 ccgacgagca gtttatgagc tggggctggc gtgtgccatt tatcttctcg gcggtgctgg
     7681 tcattatcgg cctgtatgtt cgcgtgtcgc tgcatgagtc gccggtgttt gagaaagtcg
     7741 ctaaagcgaa aaaacaggtg aagatcccgc tgggtacgct gctgaccaaa catgttcgcg
     7801 taaccgtact gggtacgttc attatgctgg caacctatac gctgttttac atcatgacgg
     7861 tctactctat gacctttagt accgccgccg cgccagttgg gcttggcctg ccgcgtaacg
     7921 aagtgttgtg gatgctgatg atggcagtta ttggttttgg cgtgatggtg ccagtcgccg
     7981 gattactggc agatgccttt ggtcgccgta aaagtatggt aattatcacc acgctgatca
     8041 tcctgttcgc gctgttcgcc tttaacccgc tgcttggttc tggcaacccg attctggttt
     8101 ttgccttcct gctgctgggg ttaagtctga tggggctgac cttcgggccg atgggcgcgc
     8161 tgttaccaga gctgttccca acagaagtgc gttacaccgg agcatcgttc tcttacaacg
     8221 tagcgtcgat tctcggggct tccgttgcac catatatcgc agcctggttg caggctaact
     8281 acgggttagg tgcggtgggg ttatatctgg cggcgatggc tggcttgacg ttaatcgccc
     8341 tgctgctgac ccatgagacg cgacatcagt cgttgtaagt atcgtgtggg cagccaccct
     8401 caccccagcc ctctccctga aagggagagg gagaaaagcg gagcgagatg ttatatcgcg
     8461 aacataacag ccctccccca gaaagtgaac ggaaaagcgg tactcgttgt ttcaacacgc
     8521 acaaaacagt cccctcgccc ctttggggag agggttaggg tgaggggaac acgctgctcc
     8581 cgaactcacc agcaaacaca atccaacctc atcacttctt catctgcgac aatatcgtcc
     8641 gacactgatt agcctcccct tcggaaggtg agatcagtgc cagtaacgcc gccgccggtg
     8701 ttaccagcgt tgccagtgcc gcagcaacag caccacgggc aatcaacggc ccggctttca
     8761 ccccagcctg cggattttta aacgtcccac gcacatacag cggcgaacgc agtgtgataa
     8821 tccgaatccc tttactctcc ggatcaatag tcaaatccag ctgttccgaa gcaaaacttg
     8881 ccgtgccggt aacattaatc aacgcgttct cagtatcgaa agcaaaaatc tgcgggcgcg
     8941 ccacgccgtt ggcaatattc agattcgccg ccgcgcagtt cacccgcacc tcatcgtcac
     9001 caaatatcgc accgacaatg tagttgccga cattcagccc aacaatctcc atcaggttgc
     9061 ggctcaccag cccgtcattc atcaacagtt tcaggttgcc gttactgttg cctaaaagtg
     9121 ctgccaccga gttaccgcta ccgcgtagtt ccgcgtcacc gttcatttcc cccagcgtct
     9181 tctgcatcag ttccacatcg ggcatcagtt ctttcagttt cagtcgacga gcctgaatat
     9241 ctgcccgccc ctgcatcggc tttttatcgc cttccagatg aatattcgcc gcaatgctgc
     9301 cgcccgccat gccaaatttc agcggttgca ggcgcaggtc agcatttttg aggatgatat
     9361 gagtagaaag atcgctaatc ggcaggctac tgccatgctc aatgcgccgc cctttgaagc
     9421 gaacatcggc atccataacg tcccatttat cggtttcgaa gcggtcataa ggcagcactt
     9481 tgcccgcagg ctgaacgctt ttttcgccct tcttctgttc agaccgtttc gacttttctg
     9541 cccctttccc ggagtcaacg ccaattaacg gtcccaggtc cgccagccgc aattgccgcg
     9601 actcgacatc accttccagt tttggtcgtg gctttccggt ggtgtagacc agagaaccgt
     9661 ggatatcgct atcgccaatt cgcccattaa aaccgcgata atcaaagacc gacgattttt
     9721 cagtgtcgat tttcgctacc agccgaccat ccgtttcaaa cggcggggta tcgggcagca
     9781 gaacgcccgt cagttcatag agatcaccca gtgaatcgcc agaaaattta agccgtaaat
     9841 cgacaccgcc catcttcatt gggtcattca cgacgccatc aaaagcaaca cgggtgttac
     9901 cagagcggaa atcagcctgt accggaaacg gcgtcccttc gccacgcagc gccagcatac
     9961 cgcctatttt tcccgtaccc gtgagcggtt caccgttata acgtccctgc gccttcaggc
    10021 caaaaacgta atcgcccacc ttttctttat ccgctttacc tttcgatcca gtaacttcgc
    10081 tgaacggcag cggcttgcct aacggatcaa caaaaatctc cagatccgct ttgcttactt
    10141 tgtcatcaat agcgatccgc ccttgatcga aaagaatatt atccagccga aacgaccatg
    10201 ccgacggctt tgcattcgcg tctttgttat catcgttggc gagattaaac gtccagttat
    10261 tgttcttttc ggagaggcga atcaggcgcg cgtcgggctt ttcgagcttg atccacggca
    10321 gccagaccgt tttggtcagc agcgccagcg gggccagcgt tgcctctacg cgtggcaaat
    10381 gcaccatcgt gacttcggga atatccggtg gattgccaag aatgatgtct tccgcgtgta
    10441 catggggcca cggcacccag ctgcgccagc cagtttcttg tttttgccgc tcccacacca
    10501 cgcccagatc gccacggata gcgaacggac gattcaactc cgcagagact ttctggttga
    10561 tggtcggttt gagtcgattc cagtcaaaag ttgcaatcaa aatgatcgcc acgacaatca
    10621 acaacaagaa agcccctgaa atcgcagcgg ttattttgcc tgccttgctc atcgttcgct
    10681 tcctcatatt cttcctgtgc cagtcctaaa gatagtccag ccaggcggaa aatgaggcag
    10741 cttatagcgc cagaaccgcc gtattcagcg tttctatcgg tgccgggcgt gaaagaaacc
    10801 agccttgtgc ggcgaatgcg ggcgagttct gaacatcacg ccactcttcc ggtgtttcta
    10861 cgccctcgac aatcaccccg cgacaatagc gattcattag atgtaaaagc tgagaaaaga
    10921 gtgtgcggcc ttccggcgac tgacgcagca tcacgaacag ctctcgcgcg attttgatgt
    10981 agtcataacg cacttcactt agcgcagaga aatttgccat cccggtgcca aaatcatcca
    11041 gccacagcgg gccaaattca cacatcgagg caaaggttga atctttcggc agacggatat
    11101 gctccaccag ttcgaaacgc agccagggaa gacgctcaat ctggcgcagg atttttggtt
    11161 gctgacgcag ggcgatgagc gtagggccat caatattgac cgatgccagc aggccgtgct
    11221 ctataaagaa gtcggctttt tgcgccagca aatcaatctg ctctttcaca acctccatcc
    11281 gatggctgac ggtgatttca gtaaaatagc gatccggcgg caggcgttgc gaagggttca
    11341 agggatgcgt gaccaccgtt aatagctcca cggccattaa ccgtccgcat gtttgataga
    11401 tcggctgcca ggtgtaagca cgctcacact gcaaccaaaa acgccgttcc tgcaagctct
    11461 cgatgcttgc ttcagggttg cttattcgct ggataacctg ccttatcatc aaagatgtcc
    11521 tgtgtttaaa agtgatgctg cccggactcg acaaagatta tcggcgcgca agggcagaac
    11581 tttaccgcct ggccggggta aatttcctca ctccacaacg gaatctttca ggaatacgtt
    11641 ctggctcaaa aaaataatgg aacactgttt taatatggtt gaccagcaaa ccaccacagc
    11701 gcaaactaac gctaattttt tacagatcag gttcacgact atgtccaaaa agattgccgt
    11761 gattggcgaa tgcatgattg agctttccga gaaaggcgcg gacgttaagc gcggtttcgg
    11821 cggcgatacc ctgaacactt ccgtctatat cgcccgtcag gtcgatcctg cggcattaac
    11881 cgttcattac gtaacggcgc tgggaacgga cagttttagc cagcaaatgc tggacgcctg
    11941 gcacggcgag aacgttgata cttccctgac ccaacggatg gaaaaccgtc tgccgggcct
    12001 ttactacatt gaaaccgaca gcaccggcga gcgtacgttc tactactggc ggaacgaagc
    12061 cgccgccaaa ttctggctgg agagtgagca gtctgcggcg atttgcgaag agctggcgaa
    12121 tttcgattat ctctacctga gcgggattag cctggcgatc ttaagcccga ccagccgcga
    12181 aaagctgctt tccctgctgc gcgaatgccg cgccaacggc ggaaaagtga ttttcgacaa
    12241 taactatcgt ccgcgcctgt gggccagcaa agaagagaca cagcaggtgt accaacaaat
    12301 gctggaatgc acggatatcg ccttcctgac gctggacgat gaagacgcgc tgtggggtca
    12361 acagccggtg gaagacgtca ttgcgcgcac ccataacgcg ggcgtgaaag aagtggtggt
    12421 gaaacgcggg gcggattctt gcctggtgtc cattgctggc gaagggttag tggatgttcc
    12481 ggcggtgaaa ctgccgaaag aaaaagtgat cgataccacc gcagctggcg actctttcag
    12541 cgccggttat ctggcggtac gtttgacggg cggcagtgcg gaaaacgcgg cgaaacgcgg
    12601 gcacctgacc gcaagtaccg ttattcagta tcgcggcgcg attatcccgc gtgaggcgat
    12661 gccagcgtaa acgcagcata tgcaaaaggc tggataaagt gttactcctc tatccagctc
    12721 atccataaat acctgatggt tgtgctaact catcaccatc aggcattact gtgcaggtgg
    12781 aatatccgtc acttcaggat gtacgtcatc cgtggcgaca gaggtggcgg cagcagcagt
    12841 cgatggggcc atgatttgat cccaggccgc ctgcaacgct ttcatgttaa attccggctc
    12901 gcctttcggc tgcagcagta ttaacgccat atcattcgac aactgctgac gcagatcctg
    12961 atttaacatc tccaccgtca ggctgttaag gaaatcctgc cgcagtttct gatactgctc
    13021 cggcgcgata tcgacgacct gattttgcaa cgaacgcatc cgctgaccca tcagaatatc
    13081 ggtatcagcc cgcgcatagg cggcaaacag tttctgcagc tccagtttct tttgcgccac
    13141 taacgcattg aactcttctt ccggcagacc tttatcgcga accttcgcca gttcacgcgc
    13201 caccagatta aggttgctgt tcagcttgtc gtttggtgat tcgatgttga tggcacactg
    13261 cgcacgcaga tacagcacac ggcagtcaaa tccaagaccg atgtctttgc tgttactggc
    13321 gcttaacgct tgctgaacat gccagaacag cgcctcgcgg gccaggtccg cacgccagta
    13381 gcgcagcagt gcggctgatt cacgaatcgg ctgccacggc gtatcccaca tgatagataa
    13441 ccggtcctga cgcaccgcgt cagtcataat actcaccgct tccgcacgca gcggagaaag
    13501 cgtcggcacc ggagctggcg tttcacgttt gcctttcagt tcgccaaacg ttttgtttat
    13561 ttggtcgaca accgagcgcg catccacgtt tcccaccacc agtagcgtca ttgcatccgg
    13621 ggtgtaccat ttctgataga aatctttaat tttttccgct tcaacgggtt gtttcagcgg
    13681 atcggcagga tcgtgaccta acaaggttga ccctttcaga cgatagcgcc accagccctc
    13741 tttagtatcg gcaggccagg ttgccaccat gtcctgactt tgcagcgcgt ggttgatggt
    13801 ttctggtgtg atggtcaatt tgccagtggc atttgccaga taagagagcg cttctttcag
    13861 caaatcgtta cggttattgg gcaaactcag attaaacagc gtggtgtcat aagagacaat
    13921 taccggcggc atcgggcgtt tagggtcgat cccctgctgc cacaatgaac gcgcctgtgc
    13981 tgcgtcaagg ccaccgcttt gcgttagcgc aatacgaggg atggcgtgac tgtaaccgct
    14041 ctgttgtgta ctttcggcga gcgaaccggt attaaccagc aggcgaattt caacacgatc
    14101 gctgggacgc tggggggtgg tcagcacttg ccactgtaaa ccgttggaaa gcgtcccctg
    14161 ttgccatgct ggatcaggct ggagcgcatc tgcctgcaca tagccagcag tggccatcat
    14221 cagcaaaccg cccgctaaaa gtcgaatttt tgtgccctgc atgtgaaccc ctgatcaact
    14281 atcctggtaa taaaaaaact gcccgttgtc ggtcagtcta aatgacgtta aaaacacttc
    14341 gtgttagacc gcaagaacat gaaaatgtca cggaagaagt gaaataaacc tgaactcacc
    14401 caggtggtaa gttcaggcac aggggtcaat tatgcgcaaa cacccgcact cggggaaggg
    14461 agtgcgggca taagtgatga gattaagagg ataattcgtg cgttttgcca tccggcgcac
    14521 gattattcag cacatcgtcc agttttttgt ggtccagttc tttcacccac ttagcaacga
    14581 caatggtcgc tacgccgtta ccgaccaggt tagtcagcgc acgagcttct gacataaagc
    14641 ggtcgatacc gaggatcagc gccagacccg ctaccggcaa atggcccacc gcagagagcg
    14701 tcgccgccag cacgataaag ccactacccg ttacccctgc cgccccttta gaagaaagca
    14761 gcaacacgat taacagcgtg atttggtgga cgatatccat ctgactgtta gtggcctggg
    14821 cgataaacac cgccgccatt gtcaggtata tcgatgtgcc atcaaggtta aacgagtagc
    14881 ctgtcgggat gaccagcccc accaccgatt tgcggcagcc gagtttctcc atcttgtcga
    14941 gcatacgcgg cagcgccgac tcggaagatg aagtccccag tacaatcagc agttcttcac
    15001 ggatgtagcg gataaatttg aagatactga aaccagtcgc tttagcgatt gaacccaata
    15061 ccagcaccac aaacaggata caggtaatgt agaaacagat aatcagctgc cccagttgca
    15121 ccagtgtgcc gacgccgtat ttaccgatgg taaacgccat tgccccgaac gcaccaatag
    15181 gcgccagacg catgatcata ttgatgatgc cgaagatgac ctgcgagaaa ctttcgatga
    15241 cgttaaaaat cagttggcct ttgctgccca gacggtggag cgcaaaacca aacagtacgg
    15301 caaacagcag cacctgcaga atgttaccgc tggcaaatgc gccaatgacg ctcgccggga
    15361 tgacatccat aatgaaggcg acaatgccct ggtctttcgc ctgatcggcg taaaccgcta
    15421 ccgctttcgc atcaagcgtt gccggatcga cgttcattcc ggcaccaggc tgcacgacgt
    15481 taacgatgat aagaccaata atcagcgcga tggtactgac aatttcaaag taaagcagtg
    15541 cgactgcgcc ggtacgaccg accgccttca tgctttccat gcccgcaatg cccgttacga
    15601 cggtacaaaa gatgacagga gcgatgatca tcttaatgag cttaacgaag ccgtcgccaa
    15661 gcggtttcat ttgctcgcct atttcaggat agaaatggcc aaggagaata ccaatggcta
    15721 tcgctgtcag gacctgaaag taaaggcttt taaacagaga ggttttcata gggtgtcctt
    15781 tagtaaaacc acaggtcttg taaggttatg gggtacctgc ggccttaaaa taacacccag
    15841 acaacatcac agaaatgtac ctggatcata attgaaacaa aaaggttaaa aagtttgagc
    15901 tggctcgcac aaaccagcac tttttaaagt tttgtaatca gtttggggta gctacttttc
    15961 ttccaggtaa ctctcttcga agatttcaat agggagtggg cgagcaaaaa ggaagccctg
    16021 ggcaatacca acgcccgctt ttgccagcca gtcgcgttgt gcttcagtct ccacgccttc
    16081 ggcaatcatt tgtaagttca ggctctgcgc cagcatgata attgcagcaa tcatgctgct
    16141 atcttccggc aagccttcaa caaacatttt gtcgattttc agtacgtcga ttggcaacga
    16201 tttcatatgc tgcagctgac gcagccctgc gtagcccatg ccgaaatcat ccagcgccac
    16261 ccgaactccg gcattgcgca gcggacggag gattgccacc gcagcatgag ggtcgtcaat
    16321 acgtcggctt tctgtcactt ccagaatcag tgttcccggc tgaatgcgat agcgggttaa
    16381 cagttccagc atatccgcca ccatattcgg gtgcatcagt tgcagcgcag agaggtttac
    16441 cgacaagggc agcataatgc cgcgctcttg ccaggctgca agcaatcgac aggactcttc
    16501 cagcacccag tgaccgacgg taaccatgag cccacagcac tcaatgcgat cgattaagcc
    16561 atccggcagg tcccaactgc catccggttg ctggatacgc agtaacactt ccgcactgac
    16621 cagtttaccg ctggtcatct cgacctgtgg ctgtaaccaa attgcaaact gatgattttc
    16681 cagtgcatta aggatatcgc tctcttccgt caaccgcttc tgggcggctt ccatctgctg
    16741 cggatcaaag aactgaatct gattcttgcc tttatggcga gcggtaaatg ccgcagaaat
    16801 agcgcgactg taaagctgtt cggcggtgag atcgccgtag aacatcgcca cgccaatgct
    16861 acagtgcgga cggagttgaa tacgttcaat cggcaggcgc tcgctcatga tagtgagcac
    16921 ttgctgacct aaggtgattg cgtgccacgg ttcctgtaca ccgttggcaa tgacagcaaa
    16981 gtcataaccg ctaatctgcg cgaggatcat acgtggcgac agtaccgatt tgagtttttc
    17041 caccagcgtc agcagcagaa tttctcgttg cgcctctttc agcacgcccg cagtatcacg
    17101 cagggtttca caggtgatga tcatcagcgc ggtggtttgt ttacgcgcga caacctgctc
    17161 cagcatctcc atcagcaagg ctttgttcgg caaatccgac accgggaagc gcatcgcatt
    17221 ttcgttctgt tcttcataat ggcgctgcag caattgctgg ttgaggttgt aactgcgcac
    17281 caacataccg atttcatcgt cctgatgcag acgcggtaat gccagttggt gaccaacaag
    17341 ctcctgggct gggatggcgt taagttcgcg agcaatattg cgtaacggat gcaaaatcag
    17401 gcggttaatg caccagctga tggcgacggt caggataagc gacaaaagta agtaaatggt
    17461 cactaacgtt gagagggtgc tcatcacgaa cttatacata cggaaggaat ccgcctgtag
    17521 taccagatag gcaattggct gcgggtttgc cggacgttcg agcgagtaaa cgcccagcga
    17581 gatttgaacc ggtagctcaa acaggcgagt aaccattacc ggcaccgggc gctctggaat
    17641 aaaactttta cgcagcgcct ggaactggtt aggcagcact acatcggcac ggctgaccac
    17701 gcctgccggt ttaatgctgg caagaatagc ttccgcttcg ggaatatcgc ctttcaaaat
    17761 agctgaagat aagggttcgc ggacagagcg agcaatgctt tccagttgcg tagccgtgtt
    17821 atagcgattc tgttggacca gatggaacag caaaacggtg caaaaaataa aaacgaacac
    17881 caggacaacg gctgccacca ttgccatctg cttgattgtt aacgagcgac ttacgcgcaa
    17941 attgtctccc cccggaaatc ccagattgcc gcccgagtat acccgatcgc agcggcatta
    18001 agagaggcgc tatctgaaaa cttaccagtc ggcgtaaggt atcagcggct gcggcggtaa
    18061 atccatgtca ccctgccatc cggcggcgga ataacgtacg tagagcagga aatggctggg
    18121 tgcgtaatct ttcgcctgct ggatatcaat tgccgtgcca acaaaccagt tggaagtaac
    18181 acgtcgttca agtaatgccc gcgccgtgta gccgaagccc tgactgctgc cgccatcgtt
    18241 ggattgccgc gcagcttctt cctgccagtc ggtcgggatc agattcatca gcggataacg
    18301 cggcatggtt ttggtgcgtg aatgcgacca cgagccagac gcccccagct cccacgacca
    18361 gttttccgtg cgctcccgcc acatcaccgg tatggcaaac gacaggtatt cctgcggact
    18421 gtagtaaccg ccctgaccga gtgagtagcc actcagatct ttgtcgtaat gccagatcat
    18481 gttgttcagg ccgattgtga cgcggcgatt gttctggtta atgaccttat agtaatagcc
    18541 cgtcatccag cgcacgcgcc agttatcttc gacattttta ccggttaact ggtcgccact
    18601 aagcgatgcc cagacgccgt ttgcttcacc tttatcgtag ctcagactta gccccacacc
    18661 gtcggcacgt acgccacccc attttttccc ggtattgctc ggggagtctt tttgcccacc
    18721 aaaggccagc aaagaactgg agatgggccg acggtgggcg ttaacggtgt aacccagcgg
    18781 cccgatatca tcgctgtaac tgatgccgcc gaccacatcc accacgttga agcccatcgg
    18841 cgtggtaccg atatcccagc tccagacgtc atttcgccag ccgaccgcca cgctggcacc
    18901 ggaatccgac tggctgcggt tgccgctaca gtcctgtaat gtacaggtgc cccagttgtc
    18961 atcccatttg ccatcggcat tagtggagaa actgccgacg ttcatattga cgaaatcact
    19021 gcgaaagaac atccgcccgt cagaatacgg cgcatccacc tgcaacatgg tagtgtgcgc
    19081 tttcagatcg gagtaaccac cggtgccgct cgaaccccag taatcgtgct caagggtgac
    19141 gttaagatcc tgctggcgat agaggtccgc cgcatcgctg cgcacgccgc gtttcagcca
    19201 gtcatctttc tcgtcgttac gggtcagtcg ggtgaatgtg tcgttatcct gcggacgcgt
    19261 cgtagtcaca ccggatgcga ccatggcgtc tttgtaggtt tccagcgcct gctttggatc
    19321 gcccgcctgc gcttcaaact tcgcaccgtc gcgcagcacc atcgcgcttt ccatcgacgg
    19381 tggctgagat tttgcctgcg ggatcaactt attaaacgtc tgctgcgccg ctgcggtatc
    19441 tccaagctgc gcctgcgcca gcgccacgcg ccgctgtgtg ttcagcgagg cgttatcggt
    19501 agcgggcagt ttcgccagct ggctacgtgc cgccgctgtg tcaccggcag caatatccac
    19561 ttccgtcagc cccagaatgg catcggcgtt agttggctcc cgcgtcagga cattctgata
    19621 tgcagcgcgg gcggcggtgt aatcacgtcg ctgctgcgcc cagtcagcca gcgtgaggtc
    19681 aatacgcgtg gaaggtggtt gctgacgcag catcgcttcc gcttcagctt ctttgccgct
    19741 ttctcgcagg cgattagcag tttccagcac ctgatcgctt tgcagtcgat taaccagctc
    19801 ctgaatattg ctgttccact gcgcgcgcgg caggctgttg atatgcgcca gcgccgctct
    19861 gtcctggtca tgaccagaga ggtacagccc gtaagcgtaa acctgctccg ggtcgttcgg
    19921 cttctgctgc gccagattgc gcattaacgt atcggcctgg ctgcgttgtc cggcctgcca
    19981 gagatcctgc gaaagtcggt aagtaatcca tacgctgccg gggtccagcg ccagtcgttg
    20041 ccgctgaagt gctgccgcct gcgcccattt gccctggttt tccagtgcct ctgcctgctg
    20101 tgccagacgg tcgttttgca ggctgcgttc gatatcatca atgctacgcc gctgactggc
    20161 agagagcgag gcgataaacg cttcagcttt ttctggcgat tgctggcggt aaatatttgc
    20221 cagcccgcgc acggcgttag tgttgccgct gtccatacgc aaggtctgct gataataacg
    20281 ttcggcggcg ggataatctt ttcgcgccat cgccacatcg cccagcccca gcactgcata
    20341 actgtcggtg ttatcgacat tacgcgcctg ctggaacagg cgttctgccc ggtcaggatt
    20401 attggctttc agcgcagcat cgccctgctg gatcgccagc cagtagcggt ttactttcag
    20461 cagactgttc catttgtcgt tgttgctgct gtgcgggtcc agtgcgaggg ctttttccag
    20521 attcgccact gcattggcgc gatcgccttt ctgagaatac gcctggccca gcgcccccag
    20581 agcttcactg tctttcgggt tcgcccgcac cgcctgttgt agttcgggaa tggctttacc
    20641 cgccatacca gagtccaccg ccgctaaacc ttgcgcacga gcgcggaaag caggatcggc
    20701 cagctgtttt tgctgttctg ccagttgcga ttgcgcagcc gccacgctat cgccatcact
    20761 aaagatcgag agatattttt tcagcgccga cacactggca tcactgacgg gcatgtcttt
    20821 aatctgcccg taccagattt tagaggcccc ttcgcgcccg gcgttcgatt ttgccatctg
    20881 ttccaggacg gcaaaacctt cgtcacggcg atcgctacta aacagcaata gcgccagatt
    20941 gttttgcagg cccgtattgc ccggtgcatc cgcattgatg cgttttaact gattaatcgc
    21001 ttcgccacgg cgagccggaa ttttcgccac cgtactccag tactcgacag caatgtcacc
    21061 ttccggcggc gcaccgttga acagtttgtt gtaactcgcc acagcttctt ctgcatgacc
    21121 ggtcgtcgcc tgcaatcgtg cctgttgcag tgcctgacga ccatccggcg tggaaagtag
    21181 catcgtagtc cgcgacgatt tatacgcatt tgaactcggc gctaactgcg acagccgatc
    21241 gagctgtttt tgcgcgccat caatatcgcc ctgacgtaac aaagaacgga aacgggcggc
    21301 aacgacgtcc gggttattcg gatcaataag ttccagccga tataacgact gttgcaccag
    21361 atcttcacga tgggtcgctt cgcctaaccg aacttgctcc agcaactgtt gctgagcggt
    21421 tggtgctgcc tcgaccatcg gcatgacggc cagaccgagg gaaagcgtga atatgtttag
    21481 tgtgaatttg cgcattcctg gccccagtca ggtaataact caccttttgt cgagaagcgg
    21541 aaacggtgtt gatcccagcc ttgtccaaac agggtcagca catagttgta ataggcatcg
    21601 ctgccgggaa agttatcggc cacgcgctgg cgctgaacgg cctgcgcatc gcggttttgt
    21661 aaaaagggca gcatggcggc agaaaaaccg accggtcctt taccctgcgc tttccccgta
    21721 gccacatcca ctttttccgg cggataaccg tttttctcag tgaatgtcgc catcggttta
    21781 aaccggttga gcatccgcgc tttttgcgga tcgctgtcag gcatcatgcc tacccacatg
    21841 taaacgcgga tagcgtcgta gctgctgatc aatgtttttt cggcttttag ctgccagcct
    21901 ttgtctttct catagcgcac ccagtctggc gaaaaacctt tcggggcggt ttccagcaat
    21961 aaacgttgat tggtttcgcg cagcgtggtc cacggcgcgc caaagcgggt gaaatactgc
    22021 gccagcgtcg gcggcaggta gctggggtta aaacgccagc tgttatcctc agcaaaaccc
    22081 actttgcccg gtaacaacat ggaacccagc ccaggcaccg tcaccacttc ctcccgcgcg
    22141 atacgtttta gcaacgcgct gccgatgtcg gtataacgct gctctttcca caaacgcccc
    22201 gcctccagca acgaccaggc catccagaca tcaccatcgg aggccgaatt gctgtccagc
    22261 acttcccact tactgttctc tttcttgccc cacagccagg cgggcaaacg ttcttttaaa
    22321 gagccctgag cgagattgtt ctgcgtccag tcgagaatat tatcgaaagc tacacggtcg
    22381 ttagccgcca gggcaaagaa cataccgtaa ctttgccctt cggaggtggt gattttgcgc
    22441 gcgtcgctgg ggtcgatgac gcgcccttcc tgactgatgt aatccttttt aaactgctcc
    22501 caggcaggcc aggtacaggc tgcctgaaca ctaaaggcag ccaacagcag catcgtcacg
    22561 attccactac gcaacacatt catcttcaat tactcgttat ccgggttaag acgacgacga
    22621 ctaataattc gcagcagacg ccacagtacc catgccagca atatcacact gatagccgcc
    22681 agcaccgcca gcagaatcgg atggtttgcc agcgcatacc acaagcgctc gaaccacggc
    22741 agatgaccta cgtaataaac gtcgccaaca cgtaggctgt tgataccgga ctcgcggatc
    22801 accgcgaccg aaccgaacat ggtggcgcgt ttgccgctat cgttcaccgc atcgttaagc
    22861 atttcataac cgcgtgggct atctgccaac agcgcaatca cgctgcgctg gtcgttatac
    22921 ggcgactgga agccaatcac cgccgccatc gcaccggaag aggtcagcgt tgaccgggtt
    22981 tctgccgcgc gatcgctctc gtccggcaca atgccgggga acggggtctg gcgcatcggt
    23041 gttttcaccc agctttcggt cgcctgcacc aataggtcga tctgcttatc gtctttcagt
    23101 ttgtccggga taccaccgat gatcatgatg tcggcatctt tgccctgaat ggtgctgcca
    23161 tcatcggtca ccgtcagatt aatcgccggg aagcccgtct gtgcgccgat aaaaccaaca
    23221 gtattcagca acgtttccat ctgtgcttcg ttaggcgctt tcggcatcac ggtgatggtt
    23281 tgcgacagat ccgccatccg gctgaatggg aagcccgcgt tagcaaaggc gcgtagatcc
    23341 ggcatcggga tgaagtggta atacttcgag aagtcgatgg tggagtcgtc accaatcacc
    23401 acatgattct gcaccggctg gaaggtaata cagttatcca ccgaaccgcc cggcatcggg
    23461 ttcatatact caaagtcgaa gcgcagctgg ttggtcgcgc ccagtttcag cgccggaata
    23521 gagacatctg ttttgccatc cagcaaacct tgtaataccg gaatccgcag cagcaggcgg
    23581 ttcgcctcct gtttgctgct caggttgaag gattgcagga actggttatt caggctgata
    23641 tccatccgcg aactgtcttt caccggcggc atggtgtagc ggtaattaat atccatatca
    23701 atgccggtac tgcgcatcag gtagagatcc ggcggcaggt ttagcgaaac gttaatcgct
    23761 gctggctcaa gaccgctgga ttgtaactgt tcttcatagg ttttcagttc gccaaatgtg
    23821 accggacgat cggtacgtac ccagttcggc gcatcgtacg gcttacgcgg tagcagcggt
    23881 ttcacttcat tcactaccac gctttcacca cggaacagaa tgttaccctg agcgatacct
    23941 ttcgctgcct gcaacaggtc tttgtcgtca cgaccaaaca ccaccagcag tttgacgtaa
    24001 ggattctgcg gatggttaat catctcaatc acaggggctt ttaccgccgg atgatcgcgc
    24061 aggaagtccg gccgtttgtc gttggttgca aagacaatgg cattgcgatc cggcagttgg
    24121 ttatagagta ccgggaagtt ctgcccacgc cagccagaac gcgaaccaaa ccacgaggcg
    24181 acaatggcag aggcttgttg cagcccaaca tccggcgcac ccgcaaagac catcggcaag
    24241 gtgttggtgc ggttatcacg cggatcaaag aatggcaccg ggaagtgtga caggtcattc
    24301 ttcacattca gggtctgata ggtcagatcc agtccactgc tccgcccaac atccagccaa
    24361 agcgtggtgc tggccgggtt ttcgcacacg tcctgataat ggccgacaaa ctccagccgc
    24421 acacggttga agtcggtaat aaacagtggg ttaatgggca tttgcgccag cgttttttta
    24481 cccaactgtt ctttggtcac tggcagcacg cccatcagct catcattgag ataaaccttt
    24541 aactgcgact ggacaggcag taacgatggc gatggcgtgt attcgaggtt gagcatcgcc
    24601 ttcgtcacca cttcatcgct gcgcatacca aactcaatgc tgccgttcgg gttaatgcca
    24661 cgtagcacca tgctgcccgg cggcggcgca atttgcgcaa aggtcagctt cacatcacgc
    24721 gaaggaccgt tctgcgccac gactggcgca tcagcgccct gcacgcccag catcacttgc
    24781 cccacctgcg gattttgttc cgtctgggcg gctacagctg gctcagcatt gatcagtggt
    24841 tgcgttgctg gcgtcgcctg cgtcatgaaa gaggggaacg cactcatccc catagccact
    24901 gcacaaatcc agaatagttt tcttttcatc gcgttatcat cattgttgag ccaaagcctg
    24961 atccgatggt tgtgccgttt cgctccgctc cgggcggcgc ggaataaacg atacaaccca
    25021 ggaaaccaga gaagtcagca cacggaatat gcccttcacc gaagaaggcg caaactccgc
    25081 cagatggcgg tagccacgga agccgagctt cagaatatcc agcagacttt ccagcggctt
    25141 atcttccggg tagctgtcct gccagagcgc ccatgtatcc gcacgggcaa acgtacactg
    25201 cacaaaatcg atatgttgct gggtggtgag cggcattaat ttcagcccaa cttcattacc
    25261 catcacgcgc gccacctggg tcgggaagac gtattcctgc tgaccgcgtt taagcaacag
    25321 attcactttc tgcccttcca gaatctgcgc ctgaccgttg atcttgatcc ccaaaccacc
    25381 gtcggagaaa tcctgaacgg tacacgagaa gaggtgacca tcttcgcggg caattgccgc
    25441 gggcatcgtc atctccacgc ggtgcgatcg gcgtacctgt ttgctttcta ccgataccgc
    25501 aactgcgccg ccaagaacaa tcaggttgta gaacacccac accatactga cgaccacggt
    25561 gagcatctcg gttggcgggc catagaagta gcgccagatg cctaccgcaa cgcccaccag
    25621 gttgagtaga acaaggaaga tgtagggccg cgagatcacc cagtcgacgt actcttcttc
    25681 caccagtcca cctttggcgg tgacgttaaa tttgcctttg tgcgggttaa tcagcgccac
    25741 cagcgtcggt ggtgcgatat accacgccag caccgtttcg tagatttcac tccagaaaga
    25801 gtggcgatat ttgccctgga tcttcgagtt ggtcaggctg gcatggatca tatgcggcag
    25861 cacgaacagg gcgatcatca acgctggcgc atagatgatg taggcatgaa gcagcaggaa
    25921 cgccagcggc gcagtcagga agatcagtcg tggaatgccc gacaagaagt ggaacatggc
    25981 gttgacgtag cacagccgct gggcgaattt cagcccttta ccggtaagcg ggttatcgag
    26041 acggaagatt tgcaccatcc cgcgtgccca gcgaatacgc tgaccgatat gcgccgacag
    26101 actttcggtc gccagccccg ccgcctgcgg aatacgcata tatgcagagg tgtagccacg
    26161 acggtgcagg cgcagagacg tgtgcgcatc ttcggttacc gtttcgacag caataccgcc
    26221 aatttcatcc aatggtttgc gacgaattac tgcacaggaa ccgcagaaga aagtggcgtc
    26281 ccacatatcg ttgccatcct gcaccagacc atagaacagc gtgccttcgt tcggtgtttt
    26341 acggaaacgc cccaggttgc gttcaaacgg gtccggcgag aagaagtggt gtggaatctg
    26401 catcatcgcc agctgtttct ctttcaggaa ccagcccatg gtcatttgca ggaacgatcg
    26461 cgttggtacg tggtcgcagt cgaaaatcga cacgaactcg cctttggcat atttcagcgc
    26521 attgttgatg ttgcccgctt tcgcatgttc atgagtggtg cgggcgatat atttcacccc
    26581 cacgttttgc gcaaactggc gaaactcttc cctgccgccg tcatcgagga tccagatgtt
    26641 cagtttgtct ttcggccagt cgatacccag cgaggcgtaa atggtatttt tcaccacatt
    26701 gagatcttcg ttgtaagtcg ggacaaagat atccaccgac ggccacagcg acatatcctt
    26761 cggcaatggc accggctgac gattcagcgg ccatactacc tggaagtagc cgagcaccag
    26821 cacaatccac gcgtacgttt cagcgaagag cagaataagc ccgcacacca ggctgaccgg
    26881 atcgtcccag ttcagcgtag aggtgtaacg ccaccagata taacggcaag aaacggtcag
    26941 cgacagcaca atcaacatta gcgccgagaa gcgccccggc atccgccgta cgatcagcgc
    27001 tacaccccac agcagcatca ggaagataaa ctgcgccagc gggttaaacg gttgagtaac
    27061 gcagattaac gccagaatca gcgagaaggt gacgatgata ccgaggatca accgccgcgc
    27121 cccggcactc aaatgaccga gctctttttt ctcatcaaga tgctgtgttt tatggctaac
    27181 gcgctcaggc agctcgttca tccattgatg gtaacgtcca cgaatattct gcagacctga
    27241 aaatgcccgc ctgcgcggtt tcggcgtttc tttgcgcgat gtaccgatca gtaaccagca
    27301 tgtttgaatg agataacgga ccgggtccag cggacgcgga cgcgaggcgt tgatatgcgg
    27361 atacaggttt ttatgttctg cgcgaatacg ctgccagcgc gggtgctcca gcggaataaa
    27421 aatccaggcc aggatcatcc agaaacagcc gagcgtcgcg ctgaaagccg acgcaccgtg
    27481 acgacgataa tcgcgataac gcccgataag ccgcgcgttg accggcggga taagcaacca
    27541 ccgggtcagg atactcatga tgcactcccg actggcgttt tcagcccgga gtagttcaac
    27601 aggcaccagt tcgccagcgt cagtatctct tcagccgcca gcgcatcact gcgatattca
    27661 cctactggtt gcttagccgc caggcattca gccatcgctt catcacgatg aatgagcatc
    27721 ggcagtaatc ggcgctggct ttgcaaccaa agctggtaaa tatcgtcctg aacctgactg
    27781 ccaatacgga agttattaat caaaatatgt gcgccatccg gcagcgcttg ctgatgcagt
    27841 cggatatggc agttggcatc cacattgacg attgccagcg agtgatcgca caaactcagc
    27901 agctggtggg ttatctgcga ggcatcacgc ggtaagtcga ttaaaatcca ctggtaacgc
    27961 ccgctggctt ttagttgctg taagccggag caaatatcgc tcagccgggt ttgccagtgc
    28021 tgtggatttt cttgttcttc aatggataac tgaccaaaag gcagcaaatc gagctgcgag
    28081 gtgtagcgca acccagcgtc acgccagtcc tggccatcca gcatcgctct ggcccagccc
    28141 tgacggtggg taaaatcaac attaaatgac aggcgcaaca agttgtccgg gcaggcatcg
    28201 accaccagaa cattttctcc caacatttgt aatgaccagg ctaatgcggc ggtgatggtt
//
LOCUS       DAZFWP020000056        28114 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFWP020000056 DAZFWP020000000
VERSION     DAZFWP020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.056"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHK0Z_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     CDS             complement(<1..209)
                     /locus_tag="ACHK0Z_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4502216.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHK0Z_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHK0Z_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU4502217.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHK0Z_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHK0Z_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU4502218.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHK0Z_RS11015"
                     /old_locus_tag="pgaptmp_002203"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHK0Z_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU4502219.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHK0Z_RS11020"
                     /old_locus_tag="pgaptmp_002204"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHK0Z_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU4502220.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHK0Z_RS11025"
                     /old_locus_tag="pgaptmp_002205"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHK0Z_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU4502221.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHK0Z_RS11030"
                     /old_locus_tag="pgaptmp_002206"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHK0Z_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU4502222.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTYDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHK0Z_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHK0Z_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4502223.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHK0Z_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHK0Z_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4502224.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHK0Z_RS11045"
                     /old_locus_tag="pgaptmp_002209"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHK0Z_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU4502225.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHK0Z_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHK0Z_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4502226.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHK0Z_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHK0Z_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU4502227.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHK0Z_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHK0Z_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU4502228.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHK0Z_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHK0Z_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU4502229.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLVG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHK0Z_RS11070"
                     /old_locus_tag="pgaptmp_002214"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHK0Z_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4502230.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHK0Z_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHK0Z_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4502231.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHK0Z_RS11080"
                     /old_locus_tag="pgaptmp_002216"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHK0Z_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU4502232.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHK0Z_RS11085"
                     /old_locus_tag="pgaptmp_002217"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHK0Z_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU4502233.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHK0Z_RS11090"
                     /old_locus_tag="pgaptmp_002218"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHK0Z_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU4502234.1"
                     /translation="MQARVKWVEGLIFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHK0Z_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHK0Z_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU4502235.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHK0Z_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHK0Z_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU4502236.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHK0Z_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHK0Z_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU4502237.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHK0Z_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHK0Z_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU4502238.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHK0Z_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHK0Z_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU4502239.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHK0Z_RS11120"
                     /old_locus_tag="pgaptmp_002224"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHK0Z_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU4502240.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHK0Z_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHK0Z_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU4502241.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHK0Z_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHK0Z_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU4502242.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHK0Z_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHK0Z_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU4502243.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTEDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHK0Z_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHK0Z_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU4502244.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="ACHK0Z_RS11145"
                     /old_locus_tag="pgaptmp_002229"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="ACHK0Z_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU4502245.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cysG"
                     /locus_tag="ACHK0Z_RS11150"
                     /old_locus_tag="pgaptmp_002230"
     CDS             26709..28082
                     /gene="cysG"
                     /locus_tag="ACHK0Z_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU4502246.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     MNWFSNH"
BASE COUNT         6800 a         7548 c         7111 g         6655 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agaaaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcataggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatggac
     9361 caattgcaat tcctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccacca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaaattaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg caggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataat tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacaaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tacccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg gcgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactg aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gagctggaag ttgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagcttcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ctggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggta gcggtctcct ctggcggcac ctctccggtt ctggcacgcc
    27121 tgttgcgcga aaaacttgaa tcactgctgc cgttgcatct gggccaggta gcgaaatacg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgggtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgatcgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaaccacc gaacagttaa tcaacgaacc gcttgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaaat gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tctt
//
LOCUS       DAZFWP020000057        27905 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFWP020000057 DAZFWP020000000
VERSION     DAZFWP020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.057"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            159..422
                     /gene="yhcN"
                     /locus_tag="ACHK0Z_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             159..422
                     /gene="yhcN"
                     /locus_tag="ACHK0Z_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU4502247.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(477..749)
                     /locus_tag="ACHK0Z_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             complement(477..749)
                     /locus_tag="ACHK0Z_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU4502248.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(841..2808)
                     /gene="aaeB"
                     /locus_tag="ACHK0Z_RS11165"
                     /old_locus_tag="pgaptmp_002233"
     CDS             complement(841..2808)
                     /gene="aaeB"
                     /locus_tag="ACHK0Z_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU4502249.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(2814..3746)
                     /gene="aaeA"
                     /locus_tag="ACHK0Z_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             complement(2814..3746)
                     /gene="aaeA"
                     /locus_tag="ACHK0Z_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU4502250.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3754..3957)
                     /gene="aaeX"
                     /locus_tag="ACHK0Z_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             complement(3754..3957)
                     /gene="aaeX"
                     /locus_tag="ACHK0Z_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU4502251.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4140..5069
                     /gene="aaeR"
                     /locus_tag="ACHK0Z_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             4140..5069
                     /gene="aaeR"
                     /locus_tag="ACHK0Z_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU4502252.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5197..6642)
                     /gene="tldD"
                     /locus_tag="ACHK0Z_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             complement(5197..6642)
                     /gene="tldD"
                     /locus_tag="ACHK0Z_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU4502253.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6798..10598)
                     /gene="yhdP"
                     /locus_tag="ACHK0Z_RS11190"
                     /old_locus_tag="pgaptmp_002238"
     CDS             complement(6798..10598)
                     /gene="yhdP"
                     /locus_tag="ACHK0Z_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU4502254.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10666..12135)
                     /gene="rng"
                     /locus_tag="ACHK0Z_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             complement(10666..12135)
                     /gene="rng"
                     /locus_tag="ACHK0Z_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU4502255.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12125..12718)
                     /gene="yhdE"
                     /locus_tag="ACHK0Z_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             complement(12125..12718)
                     /gene="yhdE"
                     /locus_tag="ACHK0Z_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU4502256.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12727..13215)
                     /gene="mreD"
                     /locus_tag="ACHK0Z_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             complement(12727..13215)
                     /gene="mreD"
                     /locus_tag="ACHK0Z_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU4502257.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13215..14318)
                     /gene="mreC"
                     /locus_tag="ACHK0Z_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             complement(13215..14318)
                     /gene="mreC"
                     /locus_tag="ACHK0Z_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU4502258.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPVNTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14384..15427)
                     /gene="mreB"
                     /locus_tag="ACHK0Z_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             complement(14384..15427)
                     /gene="mreB"
                     /locus_tag="ACHK0Z_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU4502259.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15732..17672)
                     /gene="csrD"
                     /locus_tag="ACHK0Z_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             complement(15732..17672)
                     /gene="csrD"
                     /locus_tag="ACHK0Z_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU4502260.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17824..18798
                     /locus_tag="ACHK0Z_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             17824..18798
                     /locus_tag="ACHK0Z_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU4502261.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            19776..20246
                     /gene="accB"
                     /locus_tag="ACHK0Z_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     CDS             19776..20246
                     /gene="accB"
                     /locus_tag="ACHK0Z_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU4502262.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20257..21606
                     /gene="accC"
                     /locus_tag="ACHK0Z_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     CDS             20257..21606
                     /gene="accC"
                     /locus_tag="ACHK0Z_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU4502263.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            21715..21957
                     /locus_tag="ACHK0Z_RS11240"
                     /old_locus_tag="pgaptmp_002248"
     CDS             21715..21957
                     /locus_tag="ACHK0Z_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU4502264.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21947..23398
                     /gene="panF"
                     /locus_tag="ACHK0Z_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     CDS             21947..23398
                     /gene="panF"
                     /locus_tag="ACHK0Z_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU4502265.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            23410..24291
                     /gene="prmA"
                     /locus_tag="ACHK0Z_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             23410..24291
                     /gene="prmA"
                     /locus_tag="ACHK0Z_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU4502266.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            24620..25585
                     /gene="dusB"
                     /locus_tag="ACHK0Z_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             24620..25585
                     /gene="dusB"
                     /locus_tag="ACHK0Z_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU4502267.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            25611..25907
                     /gene="fis"
                     /locus_tag="ACHK0Z_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             25611..25907
                     /gene="fis"
                     /locus_tag="ACHK0Z_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU4502268.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            25993..26877
                     /gene="yhdJ"
                     /locus_tag="ACHK0Z_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             25993..26877
                     /gene="yhdJ"
                     /locus_tag="ACHK0Z_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4502269.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            26961..27140
                     /locus_tag="ACHK0Z_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             26961..27140
                     /locus_tag="ACHK0Z_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU4502270.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRI"
     gene            complement(27143..27805)
                     /gene="acrS"
                     /locus_tag="ACHK0Z_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             complement(27143..27805)
                     /gene="acrS"
                     /locus_tag="ACHK0Z_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGU4502271.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         6816 a         7403 c         6970 g         6716 t
ORIGIN      
        1 aatacttttt tgttattaaa attcacattt ttaacactta gtatcaactg aaacagttag
       61 cgtggtatta attagttcaa taattagtgt atacttgatt ttgtgatatg ggtcactaaa
      121 caaaggccca gctaaaagat tatgtcgagg taaaaatcat gaaaatcaaa accactgtag
      181 ctgcattaag cgtactttct gttctctctt tcggtgcatt cgctgccgac tccattgatg
      241 ctgcacaagc acaaaatcgt gaagcaatcg ggaccgtatc cgtaagtggt gtggcgtctt
      301 cgccaatgga tatgcgtgaa atgctgaaca aaaaagcgga agagaaaggc gcaacggcct
      361 accagattac tgaagctcgt agcggtgaca cctggcacgc tacggctgaa ctgtacaaat
      421 aaaccctcat cgtcttgtcc gacgatattg cccccggttc gggggctttt tttgcgctaa
      481 tgacgaacat taaaacgcaa atgcccttcc agctcttcct ctgcctcatc aaacagcaat
      541 attaacgcgc caaaacggcg acgcgttttc tctcccagat gaacaaattc aatctcaagt
      601 ggtagcggca ggacatcgtt cattaacaca tcccatagtg agtcgagatc gcgtacctta
      661 tctttcgcca gaccaaaggt ttggctaaag tcacgataaa aatcctcctg actctcaatc
      721 tcatcaaaat caaaggtata aatattcatc tgttgccacc gtcacgtttc gcggcgagtt
      781 ctgccgtgca tcgctaagta tagccatgaa aaaaccgacg cttttggcgt cggttttgac
      841 ttaactatcg gtcaacgcat gttgatactt atggagcatc cccgccagcc gatgaaccgg
      901 ttccgtcacc tgcggtggcg cttgccagat gcgtagcttt tcctggtaga tttccagttc
      961 ttccagcaac tggccaaagt accgacgacg tttatcatcg ctacgggcag atatcacatg
     1021 gtctgctgtg cggcgcattt gtcggtgaaa cgccgataaa tcctcgttaa ccgggatcgg
     1081 cgcatcacgc aggcgctggt gcgcgataat catcgtcagc gccaggcgaa atttcggcaa
     1141 atcccctggg aacttattca tcagcaaaaa cagctgctga taaagtgccg ggaggtggtt
     1201 ctctttacga cgtgccacat tggtagtcat cgcggaaaca gcggcagaaa caaactgatt
     1261 aagcagtaca cgtccagtcc tgtcgcgcga tttatcccgc accagcaaaa taacggtgaa
     1321 cgcgagcaca cagccgacga tttgccctaa tgcgctgtcg agaaactgac tgaaatggaa
     1381 agtcatcggg ttatccagca cgataatatt tatggtgctg gccagcgccc ccatcgagcc
     1441 cagtcgccgt ttctgtactt ctattccgag gaagaatccc agcactgcca ggctaataca
     1501 cagcagcaac atgctctgtt gggtattagg gataatcacc aaaaagtaga gcagccctaa
     1561 cggcagcgcg gccagcgtcc cgtagataaa gtcgatcgcc accatgcgtg gattcggcaa
     1621 acgcattgcc agtgacgtca cgaccgcaat catcaccatt gcaccactgc cggaagtcca
     1681 gcccgtccac agccagaaaa gcgttcccag aatgcaggaa agtgtggttc gccagaagtt
     1741 aaccatcgca tgatgacgtt cggctgactc tactttgact tccggttcgc cttgcaggat
     1801 ctcttcttcg gtggcgttga tttttgtgtt actgataacg ccgcgcttga gaagctgata
     1861 acgcgttgcc gccgcgaccc agctataaat ggtgacaggc gtttcccgtt ccccggtcca
     1921 ggcgataact ctccgcaggc gtttgagctg cttgtggacg tcctgcgcgg tttctaccgg
     1981 cgtgtcaaaa aattcgcgga aagtatcagt gatcaattcc gggcgcgtat tctgaataag
     2041 ataagtttcg caggattggg taatcagcgt cagcgatagc gtattgatcg cttttaaacg
     2101 tcgattggcc cgcgcccagc gggaagattc catattcagg ttgctgcgca tgccttgtag
     2161 cgccgtggtg cgtcgcacca ggtcgcccca ggctttatcg acaacttcac catcgccatg
     2221 cttgatacag agttgcatta attgatattg cgcgaccagc aaactttcca gctctcgatc
     2281 cacttcttgt ttgatcgatc gcggagaaaa gagcaaatcc gccataatcg cacacacaat
     2341 accgataacg atctcgctac aacgttcgac ggcaaactgc ggcgtaagca atggttccgg
     2401 ctgaatggtg atcacaatga tcagcgcggt ataaccggcc agcccccacg cgtacgagtt
     2461 ttctattcgt accagcgagg atatccaggt acaaaaaccg gcccagatac agcacaccag
     2521 aatcatcaat aatggtgcgc ggatcatcgc aatgatgatc accagtccgg caatacagcc
     2581 aataaatgtg ccgatgatgc gcaaaaagcc acgatagcga atagcgccag aatacggttc
     2641 acctcccgca gcaaaggccg gaccggcggc aacaatcgcc gctgtcagta ccgcccagcg
     2701 tggcgtttcc agctggaagt gaaagccaac aaacagcgcc agtacaatgg cggtcgccag
     2761 ttttaccgca aagcgaatat gttggttagc aatggagaaa atacccatcg tgattaacca
     2821 aactcacgca ggcgatgggc cattttacgg aagaacgaat cctggctttc gtcgcgatct
     2881 tgtttgccag tgaccaccac tgtagcagtg gtgcccgcag gccagatgtt ctcttgctgg
     2941 ttgtcgagac gaatacgaac cggaacacgt tgcgccaaac gcacccattc aaggttagag
     3001 tcgatggtcg ccatcccttt gtcgtcacgc gtgctgctgg cgttggtgac cccagcggca
     3061 acactatcaa cagtcccttt cagcactttg ttactgccaa gcggcgtgat ctctgcacga
     3121 taccccggac gcaccccttc cagcttagtt tcttccatat aggccagtac atagaaggag
     3181 ttctgtttca ccagcgcaac cgccgttgat cctcgagtaa taaactcacc ggtatagacg
     3241 ttgaggttgg tcacccagcc atctgctggc gcgcggatca ccgtgcgttc aagatccagt
     3301 tttgccagat cgcgggtcgc ctgcgctttc gctaactgat gcagaaccgt ttgtagtacg
     3361 ttgttagcct ggtcgatctc ttcgcgagac atcgcctgca caccgagacg gttacgacgt
     3421 ccggcctcct ggcgtttctc ctgtgccagt acctgataat aagcaacatc ggcttgcgct
     3481 tcctcaagcg ccttttgata gcgcggctgg tcgatggtga acagtatctg tccttttttc
     3541 accagctggt tatcatgaac attcacctgg gtaatgagtc cagaaacgtc cggcgcgatc
     3601 gcaacgacgt cagcgctaaa gcgcgcgtca cgcgtccagg gggattcggt gtaatagacc
     3661 caggcattaa aaattgcgat gaaggccaga atgactaata cgaccgtgat ggccgtacgg
     3721 gagaattttc ttattagtgt tttcacttca acctcaaacg aacagtcgcg atatcaaata
     3781 aaacaagcag caatagagcg cggtgttgaa caacgccgga tgccagacaa agtcgtagat
     3841 acctgttggc acaagtaccc ggcgcaccag ccagaaaatc gccagtgata aaagcaattc
     3901 aaaaaatatc ggtgggaagg acagcccaaa caccacgata acgggaaaca gactcatgtt
     3961 gaccttggtt gtatagagag agcaggcgtt attattttca gcatctgtcg ccgcagagaa
     4021 gggcatggaa agccgggcga gagcaacatt gctgtagatt gatatttaat atattagcgt
     4081 aactgttatg ctgttatcta tattatgtga tctaaatcac ttttaagtca gagtgaataa
     4141 tggaacgact aaaacgcatg tcggtgtttg ccaaagtagt tgaatttggc tcttttaccg
     4201 ccgccgccag acagctacag atgagcgttt cgtccatcag tcagacggtg tcaaaactgg
     4261 aagatgagtt gcaggtaaag ctgttaaacc gtagcacacg cagcattggc ctgaccgaag
     4321 ccggtagaat ttactaccag ggctgccgtc gtatgcttca tgaagtgcag gatgttcatg
     4381 agcaactgta tgccttcaat aacaccccca tcgggacgct acgcattggc tgttcttcaa
     4441 ctatggcaca aaatgttctc gccgggctga cagccaaaat gctgaaagaa tacccaggtt
     4501 tgagcgtcaa tctggttacc ggaattccag cccccgacct gattgccgac ggtctggatg
     4561 tggtgatccg cgtcggcgcg ttgcaggatt ccagcctgtt ttcccgccgt cttggcgcga
     4621 tgccaatggt ggtgtgcgcc gcgaaaagct atctcacaca atacggcata ccggaaaaac
     4681 ccgccgattt aagtagccat tcatggcttg aatacagcgt gcggcccgac aatgaatttg
     4741 aactgattgc accggaaggg atctcgactc gcctgatccc gcaagggaga tttgtgacta
     4801 atgatccgat gacgctggtg cgctggctaa cggcgggtgc cgggatcgcc tacgtgccgc
     4861 tgatgtgggt gatcaacgag atcaatcgcg gtgagctgga gatcctgctg ccgcgttatc
     4921 agtcagatcc acgcccggtt tatgcgttat ataccgaaaa agataagctg ccgctgaagg
     4981 tacaggtcgt gatcaactcg ctgacggatt attttgttga ggtcggtaaa ttgtttcagg
     5041 agatgcacgg gcgcgggaaa gagaagtaat tcatattgta ctgttacgtt ctacaaacct
     5101 gtgccaacgg gttcccctca ccctaacccc agaggagcga ggggaccgtt cgtgcacgta
     5161 gaaagattaa ttatccttct gaaaataatg aaattattac gcagtaccgc caacagtcag
     5221 gttatcaact ttcagcgttg gctggcccac gccaaccggc aaactttgcc cttctttacc
     5281 gcagacaccc acgccgttat ccagtttcag gtcgttgcca accatcgaaa tctgctgcat
     5341 ggtttcgata ccggaaccaa tcaacgtcgc gcctttcacc ggcttcgtta ctttaccgtt
     5401 ttcaatcaga tacgcttctg aagtggagaa aacgaatttg ccggatgtaa tatccacctg
     5461 accgccacca aagttcggtg catagatacc gtactcaacg gattcaataa tttcctgcgg
     5521 ggtcgattta cccggcagca tataggtgtt ggtcatacgc ggcatgggca gatgggcgta
     5581 ggattcacgg cgaccgttgc cagtcggcgt catccccatc aaacgcgcgt tgagtttatc
     5641 ctgcatgtag cctttcagaa tgccgttctc aatcagcacg ttgtactggc ctggcgtacc
     5701 ttcgtcatca atcgccaccg aaccacggcg atcgaccatc gtgccgtcat caaccacggt
     5761 acacagttct gaagccacca gctccccgac ctgcccacta aatactgaag tgccgcggcg
     5821 gttgaagtcg ccttccagac cgtgacctac cgcttcatgc aacagcacgc ccggccaacc
     5881 tgcgccaagt actaccggca tggtgcccgc tggtgcagca acggcagaaa gattgaccag
     5941 cgccatacgc actgcttctt ttgcccatgc atccgcacgg acttcgccgt caagatcggc
     6001 aaggaagaac tcataaccaa aacgaccgcc gccgccactg gcaccgcgtt cgcgtttgcc
     6061 atcttcttcg accagaacgc tcacggaaag acgcaccagc ggacgaacat ccgccgccag
     6121 cgtgccgtcg gtggccgcaa ccagaattaa ttcataaacg ccgctgaggc tggcagtcac
     6181 ttcttgtacg cgcttgtccg cttcgcgggc aaccttatcg acgcgacgca ggatatccag
     6241 cttctcttca cggctcatgc tttgcagcgg atctaccgag gtatacaacg ggctatgctc
     6301 taccgcgccc agcgtctgta ctttgccatc accactatca cggacgatgg tgcgcgccgc
     6361 ttgcgcactc tgttccagcg ccagcaggct gatttggtca gcgtaagcaa atccggtttt
     6421 ttcaccactg attgcacgca caccaacgcc ctgatcgatg ttgtaagaac catctttaat
     6481 aatgcggtct tctaaaaccc aggattcgtg atagctcgac tgaaaataga gatcgccata
     6541 atcaaggcga cgttcggcca gttgaccgag gatcgcgaac aagtcctgat gtttcaggcc
     6601 gttcgccgct agcaattgtt cacttaccag gttcagactc atcgtttttg ctactcgtta
     6661 gttactgcag tagaggattt tttacggctg ccggatgcgg cgtgaacgcc ctatccggcc
     6721 tacagttatg ttccggctcg ttggcctttg gcaacgatta tcatatgaga ttggggcaat
     6781 tacgcgccct cgtcaaatca ttgcgctttt tctttacgcg gttggcgcaa cacttcgttg
     6841 atttgcggat cgtccagcgg acccgaaatg tgatagcgca aaatggagac tttgctccac
     6901 agcggcccca gcactttact ggctgcaaat actgccgcgc caacaatggg gttaaccgca
     6961 aaagccgcag ccacgcccac cgtcgcagaa atctctggtg cgacaaccgc ttccatattc
     7021 aggtcgcgac gtaccagatt taccgaccct ttcatggcga tatccgcctc caggccatcc
     7081 accagcgtgt cgtcggtgtg cataaccccg tctttaatcc acgcggtgct gcgaatggag
     7141 tcaaaataga acccttcgcc aaaagtgtct ctgaaatcaa aacgcagctt acgcatcagg
     7201 gcatcaacgc tcaataagcg cagcaattgc ccggcatgtc cggtattgat ttcggtaatt
     7261 tcgcctttac ccagttgagt atgaatgatg ccattcaacg tcgcctcatc tggttgccag
     7321 ggtgctttgc gccagtgtaa atcgtaatcc acattaaatg acgactggcg tatgggcgtc
     7381 gtgacaccaa aaaattctgc ggcggcatca attttctgcc cgcgcagttt tcctttcagc
     7441 gaggtacgtt cattctccgg attattaacc cattcaccat cggcagtaag ccgcgagaaa
     7501 ccagtatcaa tcagtccatt ggtcagcgtt aacgtatcgc cagaaatggt gatatcactg
     7561 tcaatgcgac cgaatttttg cccccagaac cagcactctg tgcatcgtat ttgtgcgtcc
     7621 ggccagccac ggaagttaat gcgctccgtt gtcgggaaag gtgatgacgg cgttgaatca
     7681 ccacgagttt tcgccacgct cgggttgtaa taaagatatt tgatattcgc cagccacggc
     7741 gcgttattac gcatcgctag cgtggcgttg atttcacgcc cttgcgcctc aaccagggtg
     7801 ccatttgccg tcggttgcga aacaatactc aggttattcc attgctgatt tcccagtgac
     7861 aacataggcg tacgtaacgt tatgtgttgt gggaaactcg ctgcaccacc gacactctcc
     7921 gcagcgcctt tctggaacag ggccagccac tcggcaccat tcatcggcgg catattgagt
     7981 tcaacgccac tttgttccgg caacggcggg agcgttttac tgtctgccgc ccaaatagca
     8041 cgatcgagcg tcagcttttg accaagcaac cagcggctat tgaaatggtt atccgcacca
     8101 gcctgtccgg ttaattcaaa gctgttgaga ttgccatcaa ccttaacgtt taccgctagt
     8161 ggttcacccg caggtttggc taacggtgaa ggtaagtgac tactcacatt attcagatcg
     8221 ccgttcatct ctacgttata ggtcgcgcca gcatgataag gcagatcaat gcccacttta
     8281 ccatcccacg ccacgctgcc actcaatgct tcgttcaccg cttcaggcag aacgccggtt
     8341 ttcgccggtt gccagttacc gttgaggttt accgctacct ggtaggcttt tgccccttct
     8401 ttggtggaaa aatccacgtt caacggctga ttaaaccagc ttgctgtcag tggttcactt
     8461 tgcagatcgc cattgataaa gctgaattta ccgctcaaat ttttcagggt gctgtcgagt
     8521 ggtttgataa acagactgtt attacgcagc gtcacttcac ctttcgcggt taccaactcg
     8581 ccgttcagcg ggatatcaag atgtaagcga gcattcacat cgccgtcgag ctggagttct
     8641 tgcagggtcg cacccaggga atctttcagc ggtgtctcat caaagtaagg gcctacggct
     8701 ttacccggac ctttaatgtc agcgtcaatc agcagttttt cttttgagta gtcagggatc
     8761 actgcggtga gattactcgc gcgcacgccg cccagattaa cgccatcggt tttcatccat
     8821 aaaccgtcgt taataaagtc cagttcaata tcaaggttag ttaatgcagg ccagtccggc
     8881 tggaaggcaa acttcgcgtt gcgcagcggc accagcactt caaactgacc ttcgttgtgt
     8941 ttatagggga agagttgcgg attgccacca taaaccagcg tcgcgttatc cgcttcaccg
     9001 ccctgaatcg cgccacttaa gtaatcaacc aggtctttac ccatcaagtt ttccgggaaa
     9061 tagcgccagg cttgtgaacc atcatcggta ctgatgccag ccagaatacc cagccagggt
     9121 tcatcgttag caggttgcag gtaacgaaaa ccgccgcgcg catggacggc tttggcttta
     9181 acgtcaatat tacgcccatc cagctggaaa cctttgtcat tgttcagcca gcttatagtt
     9241 gcctggccgt cggcgatttc cagtggcgca cggaataccg tttcgtaagg catctttgcc
     9301 tgcttcatcg acgcggtaag caaaccattt tcaacgctgc cggaaagcgt cccggagaag
     9361 tgttccgcac ccggtaataa tttccattgc ttccaggcca gatcgctcca cgatgcctga
     9421 aaacgggtat tgtctgccgc ctgaagcggg atatccagcg ccagagtgtt aatcttgccg
     9481 ctcggttgtg tggagcgcca aacatcaccc agtgcaggtg aaagtttcgc ggccagcggg
     9541 cgtatgccct ccaggcctgc cagctccaga ttactggcgc gaatccggag ttcgtcactg
     9601 cgtttattgt ctttgccgcc aacgtcctgt tccggtatcc aggccagcgt caatgctccg
     9661 ctcggccagg gtttaccgtc catcgtgatc cgtgtatcgg gaatagagaa ctgccagccc
     9721 ggattttcac gcgtaatatg cgcggtcaga ttatccaccg acagcgtatg cgtttccttc
     9781 tcgcccaacc agctggcacc gccctgtttc agccagacgt caccgccggt tacatcgcct
     9841 ttgtcgatcg tcatccagcc ttcaagggag aactgtgccg tttccagagc aatattgtcc
     9901 tgcatccatt taccgagcca cggcttcagg tcgatgtcat ccgcctgtag ccagacgcga
     9961 ccattgctta acaacccctc atcatcgcgc aaatccatgc gcacctgcat cacgccgtgc
    10021 tgtccggtaa ggctggagag gcttaccagg ccttccgcac ggtgtcgacg tggatcgttc
    10081 agccaggtga gttgtgggat cgccagctcg gcgcgctgac cggatggcgt cagaaaactg
    10141 acttcactgt cgcggagatc gaaatggtca aattgacgaa gaaacagatc gctgatgtga
    10201 ctggcttcca gactgtcatc gctaccaccg ctggtgatag gagtgttggt gcgaaagcgc
    10261 agctgccaga aagtgaggtc gcgaaactgc cagcgcatat gtaacaggct ctgccagaca
    10321 tccagcgcca gagtaacgcg tttaaccgaa aattcgccgc catcttttag ttctgcacgg
    10381 atgtcgtgtg cttcaagcgt cgggccaaaa ttctgccagc tggctgagag ctgactggct
    10441 tctaccggca tgccagtcgc ggattctatt ttgttgagga tttccggacg ccaggcgtca
    10501 agatgcggta aagcaatacg caggccgcta accagcaggg ctgcgatcac aacgagcgcg
    10561 gctccagtaa gcagtaaaat ccccggcaat cgcctcaccc gtcactcctt gtctgctaaa
    10621 aatgtgactc aaaaaccctt tgccggatgg cggcccagca tctgtttaca tcattacgac
    10681 gtcaaactgc tcctggttat agagcggttc aatttgtact ttaacctgtt taccaacgaa
    10741 aatttccact tccgccagcg agtgtgactc ttcgcctttc aaggcttcag ctactgccgg
    10801 agaagcatag accaggaaac ggtcggagtc gtaagcatgg tggacacgaa caatctcgcg
    10861 catgatttca tagcataccg tttccacggt tttcaccgtt ccgcgaccgt ggcaggttgg
    10921 gcattcgtta cacagtacgt gctcaatgct ttcgcgggtg cgtttacgcg tcatctccac
    10981 cagccccagc gccgaaaaac cattaacgct ggttttcacc cggtctttgc tcaacgcctg
    11041 ctccagcgag tgcagcacgc ggcggcggtg atcttcatta ttcatatcga tgaaatcaat
    11101 aatgataatc ccacccagat tacgcaaccg taactggcga gcgatagcct gcgtcgcttc
    11161 aatattggta ttgaaaatgg tgtcgtccag attgcgatga ccgacaaacg ctccggtatt
    11221 gatgtccacg gtggtcatcg cttcggtctg gtcgataatc agataaccac cggatttcag
    11281 ttctactttg cgttccagcg ctcgctggat ttcgttttcg acatcaaaga gatcgaaaat
    11341 cggctggcgt ccggtgtaat gctccagctt gctggtcatc tcgggaatgt actccgaggt
    11401 aaactccagc aatgcttcgt aagtcaggcg tgagtcaacg cgaatgcggt ccagttcggc
    11461 gtcggcgaaa tcacgcagaa cacgctgcgc cagcgccagt tcgccgtaca gctgataacg
    11521 ggtctgcggg cgttttttac gctccataac tttggtccag acgcgtttca gataagcggc
    11581 atcggaggcc agttcagcct cgccaacccc ttccgctgcg gtacggatga taaacccgcc
    11641 ctgctcgtcg caatactctg cgaccacttt tttcaggcgt tcacgttctg attcgctttc
    11701 aatacgttgg gaaacaccaa cgtgagaagc ccctggcata aagaccagat agcgagaagg
    11761 cagcgtgata tcggtggtca ggcgcgcccc cttagtgcca agcggatctt tcaccacctg
    11821 caccatcaga tcttgcccct gacgaaccag ttccgagatg tcgcgcaccg tgaattgctt
    11881 ttgttcttca cccgccacac attcggtgtg cggcatgata tcggatgcat gaagaaacgc
    11941 ggctttatcc agcccaatat ctacaaaagc cgcctgcata cccggaagta cacgacttac
    12001 acgacccttg tagatattgc ctactattcc gcgtcgcgcc tcacgttcaa tatgaatttc
    12061 ctgcagaata ccgccatcaa tatacgccac tcgcgtttcc gaaggcgtta cgtttactaa
    12121 caattcagcc gtcatgttta tcccttttct cacgcagtgc gttaaaatta cttaataatt
    12181 catacgtttc aaccagcggt aagccgacta cggcgtgata gctgccattt atcttcctga
    12241 caaaacagcc acccagcccc tgaataccgt atgcacctgc tttatctaac ggttcaccgc
    12301 tggcgacata gcccgcgatg tcttcgtctg ttaacgttct gaaagtcaca tcggtgacca
    12361 ccaggcaatc gagaatgtgc tggctgtcgg ccaacgccac tgccgtcatc acctgatggg
    12421 tctgacccga taatttgcgc aacatctgcg ccgcatgctc tgcgtcgcgc ggtttctcca
    12481 gcacttctcc attcaggata acgatagtat ccgcacccag caccgggaga tcctgcgccg
    12541 tttgcgcgac acccgcctgt gctttctcgc gcgccagacg cacaacatac tgctgcgcgc
    12601 tctcctgcgg ctgacgctgc tcctcaatgc ccgtaacaat acgttcaaag gtcacgccaa
    12661 gttgcgcaag taactcctga cgacgcggag aaccggaagc taaatacaga gaagtcatag
    12721 aaacctttat tgcactgcaa actgctgacg gactttacgc atcagcaaga aaatccacgg
    12781 ccagagcacc ccattgacta cactactcca gaacacttcc ggtctgaaag agacgttaat
    12841 cactaaaaac tctgcccaga aaacaataat atccaccacc agcgaaagca acatgacgac
    12901 cagcgcctgc tgccataatg cgaggttgcg gaaaagctgg tatttcagcg ccaccaggta
    12961 agcaatgatg ctcatcgcca atacgcgtac gccaagcgtc gagccgctga tcagatccag
    13021 tatggcaccc atcacaaaac ctgtgcccac atttacgcga tgaggcaagg ccaggatcca
    13081 atacaacaag atgagtaaca cccagtttgg ccggaaaaca atcaggttat ccggccaggg
    13141 catgatttgc agcaacagcg caatgaggaa agagagccag attacccaac gtccctggct
    13201 acgatagctc gccactattg ccctcccggc gcacgcgcag gcggttgagc accactttgc
    13261 ggcggcgtag ccctttgtgg agagcggtta gcagcaggct gtgtcggcgc agcaggcgca
    13321 gtagctgcat ttcctgtcgt cggttgctgc ggagtcggct gagcgatccc cgttgccggt
    13381 tcaggtaact ttggccccat cgcgtctggc gaaggcaata cctgcggcat catctgcatc
    13441 agacgttcat tagcaacacg atgcacctct tccggcgtca tcgggttagc gccgttacga
    13501 tccgcgcccc acagcagcag cagataacgc aaacgttgca gccctgctgt cggacgcgcc
    13561 tgaatcacag tataagcgcg ctgggtatcg agttttacgg aagagacaac cgcgaccgga
    13621 tagccttccg ggaaacgacc gcccagaccg gaagtcacca gcacatcacc aacacgaata
    13681 tccgtattca ctggtagatg ctcaagctgc aaatcatccg tacaaccgtt accggctgca
    13741 attacgcgga tatcgttgcg cagcacctgg attggcagcg cgtgggtcgc atcacaaatc
    13801 agcagcacgc gactggtcag tttagcgacg gccaccacct gaccaacaac acctttgtcg
    13861 ctgatgaccg gctggccttc ataaacgcca ttaacgctac ctttatcgat aacaacttga
    13921 tcgctataag gatcgttaac cgtggagata acctgagtca ccattttctg ctcatcctga
    13981 cgcagcgggg aacccagcag ctcgcgcaga cgcgcgttct cctgtttgta ttgtccaagc
    14041 atcagcagtt cactgttttt cagcaacagt tcctgacgta acgcccggtt ttcaagttct
    14101 agttggtcac gcgaggccag cgtctgcgat acgccatcca gcaattcacg aggagcattg
    14161 gaaacaaagt agaaaggact gacggcggta tccatataag tacggatttg actgaacgtc
    14221 cccaggcggc tgtcggcaat aataatgccg agcgccacca gcaccgccag aataaggcga
    14281 atctgtagcg acgggccacg gctaaaaatt ggcttcataa gttatgcgta ttctcgtatc
    14341 agaccaggca gggtaaacag acatttcccc tgcctgcatc cgattactct tcgctgaaca
    14401 ggtcgccgcc gtgcatgtcg atcatttcca gcgctttgcc gccaccgcgc gccacacagg
    14461 tcagcgggtc ttcagcaaca acgactggaa tgccggtttc ttccattaac aaacggtcaa
    14521 ggttacgcag cagtgcgcca ccaccggtga gcaccatgcc gcgctcggag atgtcggaag
    14581 ccagttccgg cgggcactgt tccagtgcaa ccattaccgc gctcacaata ccggtcagcg
    14641 gttcctgcag tgcttcgagg atttcgttgg agttcagggt aaaaccgcgt ggaacacctt
    14701 ctgccaggtt acggccacga acttcgattt cacggacttc atcgcccggg taagccgaac
    14761 cgatttcgtg cttgatacgt tctgcggtgg cttcaccgat cagagaaccg taattacgac
    14821 gcacatagtt gatgatagct tcgtcgaaac gatcaccacc aatgcgcacg gaagaggagt
    14881 aaaccacgcc gttcaaggag ataacagcaa cttcagtggt accaccaccg atatcaacca
    14941 ccatagaacc ggtcgcttca gaaaccggca ggccagcacc aattgcggca gccatcggtt
    15001 cttcaatcag gaagacttca cgggcaccag cgccctgcgc ggattcacga attgcgcggc
    15061 gttcaacctg ggtcgcgcca accggtacac aaaccagaac gcgcgggctt ggacgcataa
    15121 agctgttgct gtgcacttgt ttgatgaagt gctggagcat tttttcagtc acgaagaagt
    15181 cggcgataac gccgtctttc attgggcgaa tggcagcaat attgcccggc gtacggccca
    15241 gcatctgctt cgcgtcatga cctactgcag ctacgctttt cggtgaaccg gcacgatcct
    15301 gacgaatggc caccacggaa ggctcattca atacgatgcc ttgtcctttt acataaatga
    15361 gggtattcgc agtacccagg tcaatggaca agtcattgga aaacatgcca cgaaattttt
    15421 tcaacatact aagggataat cctgaaagct ggggcggaaa agaaaatccg cttactttac
    15481 caaccacacg cagcagcgac aaggcgcaaa aatcatctgc tacggtgaaa attagtgcag
    15541 ttcgtttcct ttgttacaaa tctctgcctg agtccagaaa ggcttaatgc atcagcagca
    15601 ttcctcgcct gtttgcaacc gcgtaaggtc attcatctgc atatgtgctg caacaatctg
    15661 gcgagcagac aagcacactc ccatgagacg cagcgcgcgt tattctacgt gaaaacagat
    15721 taaacggcag gttaaaccga gtatctttgt gaatattttt tcacgttagt atcaagtggc
    15781 tgtgaggacg cgaaaaaatc cccttgcccg cctgtaacac cgcgctgaat cagggtctgc
    15841 cactcgcttc gcgaacgcac accggtggcg taaacttggg tgctggtccc ggagcaggct
    15901 tccaccaggc tttgaaccag cagctggttc tccgttcgct tctcaatgtt tctgaccagc
    15961 cccggatgga gcttgagtaa ctcaacatta agttctttga tccaactggt gcttaccagc
    16021 gtcaaaccag cctggttgac ggctacccgt acccctaaag cattcactaa acgaataaca
    16081 ggttgtaaac gactgatatg ttgacctaca tcggcctctg caagttcaat aattatgcgt
    16141 tttcgttgtg atttttcaca ttgcattaac gtatcgcgca gccaacgctg aaaacgcggg
    16201 cgaatcagcg actcaacggt aacctgaatc gccagatttt cctctggcca gtaacgcaat
    16261 agtggaataa gacggctgat ttgcagacgg tcatactctt ccgataagcc aaactgcaag
    16321 accatcggca tatactccgc cgagctaacc tcttcattac catcgaagat gcggcacatg
    16381 agttcgcgat gatgaacctg accttcgcga gtaaccgccg gtttttgata aaggcgcggg
    16441 ccgccgcgac tgagcatttg ctcgataagc gtacgccagc gaacattacc gcgtcctttt
    16501 tcaggcaacg agtcatcgta aatagcccag ctattgccgc cctgcaatcc cgcattacgc
    16561 gtggcagact ctgcatgttc cattacctgc tcggtatcct gaccactacg ccaggcgcag
    16621 ataccaatgt ggatcatatc gtcgcgatcg agcattttat tgttcggcaa ggtatcgacg
    16681 gctttgatta actgaccggc gatgctctct gcctctttta acgtccggtg cggtaacagc
    16741 gcagcaaaat cactgcggtg gtaacgcgcc agcagtgcgc cagggtagcg catcataaat
    16801 gtcgacagca gattcgtcag agtgaagaac tgttcttcaa cctggctgtg cccccaggta
    16861 tcgctcaaca tattgaaatc cggcagacga atcatcatca cgatcccgtg ggtacctact
    16921 ttctcctgat cttccagtaa cgttgctaac tgattatcga aaaagagtcg gttattgagg
    16981 ccggttttca cgtcctgggc ggcataagag cggatcagcg tatcaagacg gctgtgttgt
    17041 tcgcgtgcgt tctgaatttc acgaagcagc gtatctagcg cactgctggt tctgggcggc
    17101 cattcataga tggttcccaa cacattagag ccacgctcac cgtttaagat acgagtagcc
    17161 cgggtttcca gcaattcttg cccggcaagt tgccgttgta accagcgtac cgccaggaag
    17221 agcataacaa tgataaagcc aatcgccccc gtgagcggcg cggtagtcat taaagaatgg
    17281 aaatagttgc ccatcggatc ctgataaacc agacgcagcg acatccccgg atgctttatc
    17341 aacggaacac tcagttcgcg aaacagatcg ctggagccaa ccggacgata actaccattt
    17401 ctggccaggg tataaacctg tttatcacca tggagcaggt ctacacgaac gatatctgcc
    17461 gacatcatta attcggtaat ttgtggcctt aatgtgctga agtcattcga cacaaggtgg
    17521 gtatcaatcg ccgtcgccac cgcctgaacg cgatgactaa acttatactg aattgcgttg
    17581 tagaaactta gcgaacagcc cagcaaagtc acaaaaattg ttaacccggt gagcagcgta
    17641 acaaaggccg aaaatttcgt cgttaatctc atccttgtgt taactccgat agtgaggaag
    17701 cgggcatact agcaaatcag atttatctcg caatttattg cgcttcatcg gctttgcttt
    17761 tccattagcg agtatagtct tcagaaatta ttttccaatc catcatgcac atgaggacca
    17821 cttatgcagg cgttactttt agaacagcag gacggcaaaa ctctcgcatc ggtacagaca
    17881 cttgacgaaa gtcgcctgcc ggagggcgat gtcacggttg atgttcactg gtcgagcctg
    17941 aactataaag atgcgctggc gattaccggt aagggaaaaa tcatccgtaa ttttccgatg
    18001 attcctggga tcgattttgc cggaactgta cgcaccagcg aagatccgcg ttttcatgcc
    18061 ggtcaggagg tgttactcac tggctggggc gttggtgaaa accactgggg tgggctggcg
    18121 gagcaggcgc gagtgaaagg tgactggctg gttgccatgc cgcaagggct ggacgcgcgt
    18181 aaagcaatga ttatcggtac tgccggtttt accgccatgc tgtgtgtgat ggcgctggaa
    18241 gatgccggtg ttcgcccgca ggacggggag attgtcgtga cgggtgccag tggtggcgtc
    18301 ggcagtaccg ccgtggcgct gctgcataag ttgggttatc aggtcgttgc cgtttccggt
    18361 cgcgaaagta cccatgaata cctgaaaagt ttaggtgcca gtcgtattct ccctcgtgat
    18421 gagtttgccg aatcccgtcc tctggaaaaa caagtctggg ctggggcaat tgacactgtt
    18481 ggcgacaaag tgctggcaaa agtgctggcg caaatgaatt acggcggctg cgtggcggcc
    18541 tgtggtctgg cgggtggttt tactctgcca accacggtca tgccatttat tctgcgtaat
    18601 gtccgtttgc aaggggtgga ttcagtaatg acgccaccag aacgccgcgc acaagcctgg
    18661 cagcgactgg tcgccgattt accggaatca ttctataccc aggcggcaaa agagatatct
    18721 ctgtcagagg caccgaactt tgccgaggcc atcattaata accagatcca gggtcgcacg
    18781 ctggtgaagg ttaactaacc atttagcaag gaataataag agagggaact cattttgaca
    18841 tcattattca atcggttaac gggaaaagcg gttagccgga cagctttcgt cgaacacctt
    18901 ggtcaggaag ttgtacaaca tcatccaaac tggaaagtca tgatttcgac tgaccacaaa
    18961 ttgatgcgca ttgatactcc tctaaacagc cattattgat atgcctccgt cgcctgttag
    19021 gtttatggtg ctttgcctgg gcgacactac gcttaaccta ttactgattt ctggtcccat
    19081 ggggtgaata atctgatttt atttgactac aaattaacca ctcgaaccta tttaatgctg
    19141 agcattgtca gtcggttgat tttgcgtgct ttagcattca catctatcca gacgatgcag
    19201 tgaaaattgg gtaatcccca gcaaccgctg cgtaatgtcg tctatcttgt cgcgatcctg
    19261 gcatccctac attatttgtg gtctgtgaag attatctcat tgcagcccct catcttcgca
    19321 gggctggctt tacagctttt caccttacgt tataagaagt tccgtcgatg atggcgctaa
    19381 tttcgtgaat tgtgcggctt gttgcaaatt acacggtgtt gaaggttatt tacatgttag
    19441 ctgttgatta tcttccctga taagaccagt atttagctgc caattgctac gaaatcgtta
    19501 taatgtgcga cctcgtcctc cctgacacag tttttgcgct gcggaaaagg tgacattggc
    19561 gcaacgaagg tatattttgt tttttgccgg aggatagcag cagatcgctg cacaatgtcc
    19621 gtcaagtcta acattgacac tctggggcaa aatagaccgg cgtcccggcc tgctggaatt
    19681 tatcgctatg catacagctg tcggggcata cgctttacag acggcggtga aacgcctgtc
    19741 acaatcacac taaacaaaga gtacggaacc cactcatgga tattcgtaag attaaaaaac
    19801 tgatcgagct ggttgaagaa tcaggcatct ccgaactgga aatctccgaa ggcgaagagt
    19861 cagtacgcat cagccgtgca gctcctgccg caagtttccc tgtgatgcaa caagcttacg
    19921 ctgcaccaat gatgcagcag ccagctcaat ctaacgcagc cgctccggcg accgttcctt
    19981 ccatggaagc gccagcagca gcggaaatca gtggtcacat cgtacgttcc ccgatggttg
    20041 gtactttcta ccgcacccca agcccggacg caaaagcgtt catcgaagtg ggtcagaaag
    20101 tcaacgtggg cgataccctg tgcatcgttg aagccatgaa aatgatgaac cagatcgaag
    20161 cggacaaatc cggtaccgtg aaagcaattc tggtcgaaag tggacaaccg gtagaatttg
    20221 acgagccgct ggtcgtcatc gagtaacgag gcgaacatgc tggataaaat tgttattgcc
    20281 aaccgcggcg agattgcatt gcgtattctt cgtgcctgta aagaactggg catcaagact
    20341 gtcgctgtgc actccagcgc ggatcgcgat ctaaaacacg tattactggc agatgaaacg
    20401 gtctgtattg gccctgctcc gtcagtaaaa agttatctga acatcccggc aatcataagc
    20461 gccgctgaaa tcaccggcgc agtagcaatc catccgggtt acggcttcct ctccgagaac
    20521 gccaactttg ccgagcaggt tgaacgctcc ggctttatct tcattggccc gaaagcagac
    20581 actattcgcc tgatgggtga caaagtgtcg gcaatcgcgg cgatgaaaaa agcgggcgtt
    20641 ccttgcgtac caggttctga cggcccgctg ggcgacgata tggataaaaa ccgtgccatt
    20701 gctaaacgca taggttatcc ggtgattatc aaagcctccg gcggcggcgg cggtcgtggt
    20761 atgcgtgtag tgcgcggcga cgctgaactg gcacaatcca tctccatgac ccgtgcggaa
    20821 gcgaaagccg cattcaataa cgatatggtt tacatggaga aatacctgga aaatcctcgc
    20881 cacgtcgaga ttcaggtact ggctgacggt cagggcaacg ctatctatct ggcggaacgt
    20941 gactgctcta tgcagcgtcg tcaccagaaa gtggtcgaag aagcaccagc accgggaatt
    21001 acaccggaac tgcgtcgcta catcggcgaa cgttgtgcta aagcgtgtgt tgatatcggc
    21061 tatcgcggtg caggtacttt cgagttcctg ttcgaaaacg gcgagttcta tttcatcgaa
    21121 atgaacaccc gtattcaggt agaacacccg gttacagaaa tgatcaccgg cgttgacctg
    21181 atcaaagaac agctgcgtat cgctgccggt caaccgctgt cgatcaagca agaagaagtt
    21241 cacgttcgcg gccatgcggt ggaatgtcgt atcaacgccg aagatccgaa caccttcctg
    21301 ccaagcccag gcaaaatcac ccgtttccac gcacctggcg gttttggagt gcgttgggag
    21361 tctcatatct acgcgggcta caccgtaccg ccgtactatg actcaatgat cggtaagctg
    21421 atttgctacg gtgaaaaccg tgacgtggcg attgctcgca tgaagaatgc gctgcaggag
    21481 ctgatcatcg acggtatcaa aaccaacgtt gatctgcaga tccgcatcat gaatgacgag
    21541 aacttccagc atggtggcac taacatccac tatctggaga aaaaactcgg tcttcaggaa
    21601 aaataagact gcaaaagcgt caaaaggccg gattttccgg ccttttttat tactggggat
    21661 cgacaacccc cataaggtac aatcctcgct ttcttcaccc atcagggaca aaaaatggac
    21721 actcgttttg ttcaggccca taaagaggcg cgctgggcgc tggggctgac ccttttgtat
    21781 ctggcagttt ggttagtagc cgcttactta cctggcgttg cccccggttt taccggcttt
    21841 ccgcgctggt ttgagatggc ctgcatcctg acgccgctac tgtttattgg actgtgctgg
    21901 gcgatggtga aatttatcta tcgcgatatc ccactggagg atgacgatgc agcttgaagt
    21961 aattctaccg ctggtcgcct atctggtggt ggtgttcggt atctcggttt atgcgatgcg
    22021 taaacggagc accggcacct tccttaatga gtatttcctc ggcagccgct ctatgggcgg
    22081 tattgtgctg gcgatgacgc tcaccgcgac ctatatcagt gccagttcgt ttatcggcgg
    22141 gccaggagct gcttataaat acgggctggg ctgggtattg ctggcgatga ttcagcttcc
    22201 tgcagtctgg ctttcactcg gtattctcgg caagaagttt gcgattcttg cgcgccgcta
    22261 caatgcagtg acgctgaacg atatgctgtt tgcccgctac cagagtcgtc ttctggtgtg
    22321 gctggcgagt ttgagtttgc tggttgcgtt cgttggtgcg atgaccgtgc agtttatcgg
    22381 cggtgcgcgc ctgctggaaa ccgcggcggg tattccttat gaaaccgggc tgctgatttt
    22441 tggtatcagc attgcgttat ataccgcctt tggtggcttt cgcgccagcg tgctgaacga
    22501 caccatgcaa gggcttgtga tgctgattgg caccgttgtg ctgcttattg gcgtggtaca
    22561 tgccgctggc ggcttaagca acgcagtaca gaccttgcaa accatcgatc cgcaactggt
    22621 tacgccacaa ggcgctgacg atattctgtc gcctgccttt atgacgtcgt tctgggtact
    22681 ggtgtgcttt ggcgtgattg gcctgccgca tactgcggtg cgctgtatct cttataaaga
    22741 cagcaaagca gtacaccggg ggatcatcat cggtacgatt gtagtcgcaa tcctgatgtt
    22801 cggtatgcac ctggccggag cgttaggtcg tgcagtgatc cccgatctca ccgtaccgga
    22861 tctggtgatc ccgacgttaa tggtaaaagt gctgccaccg tttgctgccg gaatcttcct
    22921 ggctgcacct atggctgcga tcatgtcgac gatcaatgcc cagttgctgc aaagttccgc
    22981 tacgatcatt aaagatctct atctgaatat ccgtccggat caaatgcaaa acgagacgcg
    23041 tctgaagcgg atgtcggcgg taattacgtt agttctcggc gcgttgctgc tgcttgccgc
    23101 ctggaagccg ccagaaatga tcatctggct gaatttgctg gccttcggtg ggctggaagc
    23161 cgttttcctg tggccgctgg tgctgggtct ttactgggaa cgcgccaacg ccaaaggcgc
    23221 gctaagtgcg atgatcgttg gcggcgtgct gtatgccgta ctcgcgacgc tgaatattca
    23281 gtacctgggc ttccacccta ttgtgccctc gttactacta agtttgctgg ctttcctggt
    23341 cggaaaccgt ttcggtacat ccgtcccgca agctaccgtt ttgactactg ataaataaag
    23401 agttttgcca tgccttggat ccaactgaaa ctgaacacca ccggcgcgaa cgcggaagat
    23461 cttagcgatg cgctgatgga agcgggtgcc gtttctatca cttttcagga tacccacgat
    23521 acgccagtat ttgagccgct gccgggcgaa acgcgcctgt ggggcgacac cgatgtgatt
    23581 ggtctgttcg acgctgaaac cgatatgaac gacgtggtgg cgattctgga aaaccatccg
    23641 ctgctcggcg caggcttcgc gcataaaatc gaacaactag aagataaaga ctgggagcgc
    23701 gaatggatgg ataatttcca cccgatgcgc tttggtgaac gactgtggat ctgccctagc
    23761 tggcgtgatg tgccggacga aaacgccgtc aacgtgatgt tagatccagg gctggcgttt
    23821 ggtacgggta cccatccaac cacctctctg tgcctgcaat ggctcgacag cctcgattta
    23881 accggtaaaa cagtcatcga ctttggctgt ggttccggca ttctggcgat cgcggcgctg
    23941 aaactgggtg cagcaaaagc cattggtatt gatatcgatc cgcaggcgat tcaggccagc
    24001 cgcgacaacg ccgaacgtaa tggcgtttct gaccgtctgg aactctactt accgaaagat
    24061 cagccagaag aaatgaaagc cgacgtggtg gtcgctaaca tccttgcagg cccattacgt
    24121 gaactggcac cgttaatcag cgtcctgccg gtttcaggcg gtttgctggg cctttccggt
    24181 attctggcaa gccaggcaga gagcgtttgt gaagcttatg ccgatagctt cgcactggac
    24241 ccggtcgtgg aaaaagaaga gtggtgccgt attaccggtc gtaagaatta accttcgcat
    24301 cgccgtaggg tgacgcgggg cgaagtgcga gcaagctcac aaaaggcacg taaatttgcc
    24361 ggttatttac gcaaatttgc gtgccaaaat tttcattcat aaagaaaaat tgagaactta
    24421 ctcaaatttc tttgagtgta aattttagtc actattttct aatatgatga tttttatgag
    24481 taattatcgc accacgctca ttttaaatgc aattctttga tccatctcag aggattggtc
    24541 aaagtttggc ctttcatctc gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag
    24601 tatggtcatt tcttaactca tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc
    24661 agcgcccatg gctggcatta cagacagacc ttttcggacg ttgtgctacg agatgggagc
    24721 cggattgaca gtatccgaga tgatgtcttc taacccacag gtttgggaaa gcgacaaatc
    24781 tcgtttacgg atggtgcaca ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag
    24841 cgatccgaaa gaaatggcag atgcagcacg tattaacgtg gaaagcggtg cccagattat
    24901 tgatatcaat atgggttgcc cggctaaaaa agtgaatcgc aagctcgcag gttcagccct
    24961 cttgcagtac ccggatgtcg ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt
    25021 tcctgttacc ctgaagattc gcaccggctg ggcaccggaa caccgtaact gcgaagagat
    25081 tgcccaactg gctgaagact gtggcattca ggctctgacc attcatggcc gtacacgcgc
    25141 ctgtttgttc aatggagaag ctgagtacga cagtattcgg gcagttaagc agaaagtttc
    25201 cattccggtt atcgcgaatg gcgacattac tgacccgctt aaagccagag ctgtgctcga
    25261 ctatacaggg gcggatgccc tgatgatagg ccgcgcagct cagggaagac cctggatctt
    25321 tcgggaaatc cagcattatc tggacactgg ggagttgctg cccccgctgc ctttggcaga
    25381 ggttaagcgc ttgctttgcg cgcacgttcg ggaactgcat gacttttatg gtccggcaaa
    25441 agggtaccga attgcacgta aacacgtttc ctggtatctc caggaacacg ctccaaatga
    25501 ccagtttcgg cgcacattca acgccattga ggatgccagc gaacagctgg aggcgttgga
    25561 ggcatacttc gaaaattttg cgtaaacaga aataaagagc tgacagaact atgttcgaac
    25621 aacgcgtaaa ttctgacgta ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc
    25681 aaaaacccct gcgtgactcg gttaaacagg cactgaagaa ctattttgct caactgaatg
    25741 gtcaggatgt gaatgacctc tatgagctgg tactggctga agtagaacag cccctgttgg
    25801 acatggtgat gcaatacacc cgtggtaacc agacccgtgc tgcgctgatg atgggcatca
    25861 accgtggtac gctgcgtaaa aaattgaaaa aatacggcat gaactaattc aggttagcta
    25921 aatgcttgat taaaaaggcg ctactcggca tggggaagcg ccttttttat aggtgtcaca
    25981 aagggagtga ccatgagaac aggatgtgaa ccgacccggt ttggtaatga agctaagacc
    26041 attattcacg gtgatgcgct tgccgaactt aaaaagatcc ccgccgaaag tgtcgatctg
    26101 atctttgccg acccaccata taacatcggt aaaaattttg atggtctgat cgaagcctgg
    26161 aaagaagatc tgtttatcga ctggctgttt gaagtgattg cagagtgcca ccgcgttctg
    26221 aaaaagcagg gcagcatgta catcatgaac agcacggaaa acatgccctt tatcgatctc
    26281 cagtgccgca agctttttac catcaaaagt cgcatcgtct ggtcatatga cagttctgga
    26341 gtacaggcga aaaaacacta cggctccatg tacgaaccca tcctgatgat ggtgaaagac
    26401 gcaaagaact acacattcaa cggtgatgct attctggtag aagccaaaac cggatcgcag
    26461 cgcgcgttga tcgattatcg caaaaatcct ccacagccat acaatcatca aaaagtaccg
    26521 ggtaacgtct gggattttcc gcgcgtgcgt tatttgatgg atgaatatga gaaccacccg
    26581 acgcaaaaac cggaagcctt actgaaacgc attattctcg cctcttccaa cccaggcgat
    26641 atcgttctcg acccgtttgc cggtagcttt actaccggtg ccgtagccgt ctccagcggg
    26701 cgaaaattca ttggtattga gatcaacagc gagtacatca aaatggggct tcgacggctg
    26761 gatgtcgcgt cgcattactc tgcggaagaa ctggcgaaag tgaaaaaaag aaagaccggc
    26821 aacctgtcaa aacgaagccg gttaagcgaa gttgaccccg atctcattgc aaagtaaagg
    26881 aagtgtaagc ctggttttca gattattcat ttcgtgtata tttcctgcca gacttggtta
    26941 aacatacaca ggctcaggta atgattcgca agtattggtg gctcgtcgtt ttcgctgtct
    27001 tcgtttttct gtttgatact ttactgatgc agtggattga actgctggca acagaaacag
    27061 acaaatgccg caatatgaac tcagttaatc cactaaaact ggttaactgt gacgaactga
    27121 attttcagga cagaatatga atttacatga cacttaattc attcgtttga tgaattaatt
    27181 tcgttatgtt ttcatctggc atgaacattc ttaatacgtt atcgaccaga gcgggggctt
    27241 gtttataaag atcataaccc gccatattca ttaaccagtt ttgaacaatt ccgctgaagg
    27301 caccattaat aataatcatc acaacatcta aatcgaggtt atttgctaca caaccttgtt
    27361 gctgacacgc ctgcaatact tcgcggagag tctgcggatt gaagcccatc ttttcgcgta
    27421 tcactccctc ggccagcatc tcatcattaa attcacattt gtgatataag attttcagca
    27481 acgcctgctg gcggggaatt ttggcaatat attgcaagcc gacaatcaat ttttcacgca
    27541 attgttgaaa cgggtcatgc tctaatccag ccgtcaagtg ttcctggatt aactcccgca
    27601 atgaaggctg ttgcaaccac atctcattaa acagttgagt cttgttttcg aagtgccagt
    27661 agatagcgcc acgcgtaacg ttagcagcgt cggcaatgtc gttgagtgtc gtcttgctta
    27721 cgccatgctg cgcaaactgg gcgatggcag tttcaatcag ttcttgccgg gtcttcagag
    27781 cttcggcttt ggttcttttt gccatgatta attattcagg aaataaatat attcgacaca
    27841 gagtgagaaa atagcgaagg ttaatctatc acctaatgtg tatttatacg agaggctaat
    27901 attga
//
LOCUS       DAZFWP020000058        27205 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFWP020000058 DAZFWP020000000
VERSION     DAZFWP020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.058"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..207
                     /locus_tag="ACHK0Z_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             <1..207
                     /locus_tag="ACHK0Z_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502272.1"
                     /translation="KIRNHASEFTDLIILILIYFEKGIGIYAVFLKILIIGMDFRARK
                     GFYMDTLLIISSSHHQQFTSLFRV"
     gene            300..2723
                     /gene="pgaA"
                     /locus_tag="ACHK0Z_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             300..2723
                     /gene="pgaA"
                     /locus_tag="ACHK0Z_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGU4502273.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2732..4750
                     /gene="pgaB"
                     /locus_tag="ACHK0Z_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             2732..4750
                     /gene="pgaB"
                     /locus_tag="ACHK0Z_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGU4502274.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4743..6068
                     /gene="pgaC"
                     /locus_tag="ACHK0Z_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             4743..6068
                     /gene="pgaC"
                     /locus_tag="ACHK0Z_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU4502275.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6070..6483
                     /gene="pgaD"
                     /locus_tag="ACHK0Z_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /pseudo
     CDS             6070..6483
                     /gene="pgaD"
                     /locus_tag="ACHK0Z_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(6533..7321)
                     /gene="phoH"
                     /locus_tag="ACHK0Z_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /pseudo
     CDS             complement(6533..7321)
                     /gene="phoH"
                     /locus_tag="ACHK0Z_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
     gene            complement(7940..9211)
                     /gene="efeB"
                     /locus_tag="ACHK0Z_RS11310"
                     /old_locus_tag="pgaptmp_002262"
     CDS             complement(7940..9211)
                     /gene="efeB"
                     /locus_tag="ACHK0Z_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU4502276.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9217..10344)
                     /gene="efeO"
                     /locus_tag="ACHK0Z_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             complement(9217..10344)
                     /gene="efeO"
                     /locus_tag="ACHK0Z_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU4502277.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10402..11232)
                     /gene="efeU"
                     /locus_tag="ACHK0Z_RS11320"
                     /old_locus_tag="pgaptmp_002264"
     CDS             complement(10402..11232)
                     /gene="efeU"
                     /locus_tag="ACHK0Z_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU4502278.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11775..13283)
                     /gene="putP"
                     /locus_tag="ACHK0Z_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             complement(11775..13283)
                     /gene="putP"
                     /locus_tag="ACHK0Z_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU4502279.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13442..13651)
                     /locus_tag="ACHK0Z_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             complement(13442..13651)
                     /locus_tag="ACHK0Z_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502280.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKINQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13706..17668
                     /gene="putA"
                     /locus_tag="ACHK0Z_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             13706..17668
                     /gene="putA"
                     /locus_tag="ACHK0Z_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4502281.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAAQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLARRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17708..18346)
                     /gene="rutR"
                     /locus_tag="ACHK0Z_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             complement(17708..18346)
                     /gene="rutR"
                     /locus_tag="ACHK0Z_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU4502282.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18634..19725
                     /gene="rutA"
                     /locus_tag="ACHK0Z_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             18634..19725
                     /gene="rutA"
                     /locus_tag="ACHK0Z_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU4502283.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19725..20417
                     /gene="rutB"
                     /locus_tag="ACHK0Z_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             19725..20417
                     /gene="rutB"
                     /locus_tag="ACHK0Z_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU4502284.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20429..20815
                     /gene="rutC"
                     /locus_tag="ACHK0Z_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             20429..20815
                     /gene="rutC"
                     /locus_tag="ACHK0Z_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGU4502285.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20823..21623
                     /gene="rutD"
                     /locus_tag="ACHK0Z_RS11360"
                     /old_locus_tag="pgaptmp_002272"
     CDS             20823..21623
                     /gene="rutD"
                     /locus_tag="ACHK0Z_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU4502286.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLQQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21633..22223
                     /gene="rutE"
                     /locus_tag="ACHK0Z_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             21633..22223
                     /gene="rutE"
                     /locus_tag="ACHK0Z_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU4502287.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HSDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22234..22728
                     /gene="rutF"
                     /locus_tag="ACHK0Z_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             22234..22728
                     /gene="rutF"
                     /locus_tag="ACHK0Z_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU4502288.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22749..24077
                     /gene="rutG"
                     /locus_tag="ACHK0Z_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             22749..24077
                     /gene="rutG"
                     /locus_tag="ACHK0Z_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU4502289.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGMNPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(24160..24333)
                     /gene="ymdF"
                     /locus_tag="ACHK0Z_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             complement(24160..24333)
                     /gene="ymdF"
                     /locus_tag="ACHK0Z_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4502290.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24706..25302
                     /gene="wrbA"
                     /locus_tag="ACHK0Z_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             24706..25302
                     /gene="wrbA"
                     /locus_tag="ACHK0Z_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4502291.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPIATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25323..25550
                     /gene="yccJ"
                     /locus_tag="ACHK0Z_RS11390"
                     /old_locus_tag="pgaptmp_002278"
     CDS             25323..25550
                     /gene="yccJ"
                     /locus_tag="ACHK0Z_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU4502292.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25588..26829)
                     /gene="agp"
                     /locus_tag="ACHK0Z_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             complement(25588..26829)
                     /gene="agp"
                     /locus_tag="ACHK0Z_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU4502293.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27122..>27205)
                     /locus_tag="ACHK0Z_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             complement(27122..>27205)
                     /locus_tag="ACHK0Z_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502294.1"
                     /translation="PELFAQAYKLKNKEMNAQLDYLNRYAI"
BASE COUNT         6635 a         7048 c         6896 g         6626 t
ORIGIN      
        1 aaaatccgaa atcatgcatc ggaatttact gatttaatta ttttaatcct aatttatttt
       61 gaaaaaggca ttgggattta tgccgtattc ctgaagatcc tcatcattgg aatggatttt
      121 cgggcgagaa aaggatttta tatggacact ctgctcatca tttcttcttc tcatcatcaa
      181 caattcacgt ctctcttccg cgtttaataa cggattatga ggtgcaaaaa tatctttctt
      241 ttcagttacc tgtaattaga tacagagaga gattttggca atacatggag taatacagga
      301 tgtattcaag tagcagaaaa aggtgcccga aaaccaaatg ggctttgaaa cttcttactg
      361 ccgcattttt agcagcgagt cccgcggcga agagtgctgt taataacgcc tatgatgcat
      421 tgattattga agctcgcaag ggtaatactc agccagcttt gtcatggttt gcactaaaat
      481 cagcactcag caataaccaa attgctgact ggttacagat tgccttatgg gccgggcaag
      541 ataaacaggt tattaccgtt tacaaccgct accgtcatca gcaattacca gcgcgtggtt
      601 atgcagctgt cgccgtcgct tatcgtaacc tgcaacaatg gcaaaactcg cttacactgt
      661 ggcaaaaggc gctctctctg gagccgcaaa ataaggatta tcaacgggga caaattttaa
      721 ccctggcaga tgctggtcac tatgatactg cgctggttaa acttaagcag cttaactctg
      781 gagcaccgga caaagccaat ttactcgcag aagcctatat ctataaactg gcggggcgtc
      841 atcaggatga attacgggcg atgacagagt cattacctga aaatgcatct acgcaacaat
      901 atcccacaga atacgtgcag gcattacgta ataatcaact tgctgccgcg attgacgatg
      961 ccaatttaac gccagatatt cgcgctgata ttcatgccga actggtcaga ctgtcgttta
     1021 tgcctacgcg cagtgaaagt gaacgttatg ccattgccga tcgcgccctc gcccaatacg
     1081 ctgcattaga aattctgtgg cacgataacc cagaccgtac tgcccagtac cagcgtattc
     1141 aggttgatca tcttggcgcg ttattaactc gcgatcgtta taaagacgtt atttctcact
     1201 atcagcgatt aaaaaagacg gggcaaatta ttccgccctg ggggcaatat tgggttgcat
     1261 cggcttatct caaagatcat cagccgaaaa aagcacagtc aataatgacc gagctctttt
     1321 atcacaagga gaccattgcc ccggatttat ccgatgaaga acttgcggat ctcttttaca
     1381 gccacctgga gagtgaaaat tatccgggcg cgctaactgt cacccaacat accattaata
     1441 cttcgccgcc tttccttcgg ttaatgggca cgcctacgag catcccgaat gatacctggt
     1501 tacaggggca ttcgtttctc tcaaccgtag caaaatatag taatgatctt cctcaggctg
     1561 aaatgacagc cagagagctt gcttataacg caccaggaaa tcagggactg cgcattgatt
     1621 acgcgagtgt gttacaagcc cgcggttggc ctcgtgcagc agaaaatgaa ttaaaaaaag
     1681 cagaagtgat cgagccacgt aatattaatc tggaggttga acaagcctgg acagcattaa
     1741 cgttacaaga atggcagcag gcagctgtct taacgcacga tgttgtcgaa cgtgaaccgc
     1801 aagatcccgg cgttgtacga ttaaaacgtg cggttgatgt acataatctt gcagagcttc
     1861 gtatcgctgg ctcaacagga attgatgccg aaggcccgga tagtggtaaa catgatgtcg
     1921 acttaaccac catcgtttat tcaccaccgc tgaaggataa ctggcgcggt tttgctggat
     1981 tcggttatgc cgatggacaa tttagcgaag gaaaagggat tgttcgcgac tggcttgcgg
     2041 gtgttgagtg gcggtcacgt aatatctggc tcgaggcaga gtacgctgaa cgcgttttca
     2101 atcatgagca taaacccggc gcgcgcctgt ctggctggta tgattttaat gataactggc
     2161 gtattggttc gcaactggaa cgcctctctc accgcgttcc attacgggca atgaaaaatg
     2221 gtgttacagg caacagtgct caggcttatg ttcgctggta tcaaaatgag cggcgtaagt
     2281 acggtgtctc ctgggctttc actgattttt ccgacagtaa ccagcgtcat gaagtctcac
     2341 ttgagggtca ggaacgcatc tggtcttcac catatttgat tgtcgatttc ctacccagtc
     2401 tgtattacga acaaaataca gaacacgata ccccatacta caaccctata aaaacgttcg
     2461 atattgttcc ggcatttgag gcaagccatt tgttatggcg aagctatgaa aatagctggg
     2521 agcaaatatt cagcgcaggt gttggtgcct cctggcaaaa acattatggc acggatgtcg
     2581 tcacccaact cggctacggg caacgcatta gctggaatga cgtgattgat gctggcgcaa
     2641 cgctacgctg ggaaaaacga ccttatgacg gtgacagaga acacaactta tacgttgaat
     2701 tcgatatgac attcagattt taaggataaa tatgttacgt aatggaaata aatatctcct
     2761 gatgctggtg agtataatta tgctcaccgc gtgcattagc cagtcaagaa catcatttat
     2821 accgccacag gatcgcgaat ctttactcgc cgagcaaccg tggccgcata atggttttgt
     2881 agcgatttca tggcataacg ttgaagacga agctgccgac cagcgtttta tgtcagtgcg
     2941 gacatcagca ctgcgtgaac aatttgcctg gctgcgcgag aacggttatc aaccggtcag
     3001 tattgctcaa attcgtgaag cacatcgagg aggaaaaccg ctaccggaaa aagctgtagt
     3061 gctgactttt gatgacggct accagagttt ttatacccgc gtcttcccaa ttcttcaggc
     3121 cttccagtgg cctgctgtat gggcccccgt cggcagttgg gtcgatacgc cagcggataa
     3181 acaagtaaaa tttggcgatg agttggtcga tcgagaatat tttgccacgt ggcaacaagt
     3241 gcgagaagtt gcgcgttccc gactcgttga gctcgcttct catacatgga attctcacta
     3301 cggtattcag gctaatgcca ccggcagctt attgcctgta tatgtaaatc gtgcatattt
     3361 tactgaccac gcacggtatg aaaccgcagc agaataccgg gaaagaattc gtctggatgc
     3421 tgtaaaaatg acggaatacc tgcgtacaaa ggttgaggta aatccacacg tttttgtttg
     3481 gccttatggc gaagcgaatg gcatagcgat agaggaatta aaaaaactcg gttatgacat
     3541 gttcttcacc cttgaatcag gtttggcaaa tgcgtcgcaa ttggattcca ttccgcgggt
     3601 attaatcgcc aataatccct cattaaaaga gtttgcccag caaattatta ccgtacagga
     3661 aaaatcacca caacggataa tgcatatcga tcttgattac gtttatgacg aaaacctcca
     3721 gcaaatggat cgcaatattg atgtgctaat tcagcgggtg aaagatatgc aaatatcaac
     3781 cgtgtatttg caggcatttg ctgatcccga tggtgatggg ctggtcaaag aggtctggtt
     3841 tccaaatcgt ttgctaccaa tgaaagcaga tatttttagt cgggttgcct ggcaattacg
     3901 tacccgctca ggtgtaaaca tctatgcgtg gatgccggta ttaagctggg atttagatcc
     3961 cacattaacg cgagtaaaat acttaccaac aggggagaaa aaagcacaaa ttcatcctga
     4021 acaatatcac cgtctctctc ctttcgatga cagagtcaga gcacaagttg gcatgttata
     4081 tgaagatctt gccggacatg ctgcttttga tggcatattg ttccacgatg atgctttgct
     4141 ttcagattat gaagatgcca gtgcaccggc tatcacggct tatcagcaag caggctttag
     4201 cgggagtctg agcgaaattc gacaaaaccc ggagcaattt aaacagtggg cccgctttaa
     4261 aagtcgtgcg ttaactgact tcactttaga acttagtgcg cgcgtaaaag ccattcgcgg
     4321 tccacatatt aaaactgcac gaaatatttt tgcacttccg gtaatacaac ctgaaagtga
     4381 agcctggttt gcacagaatt atgctgattt cctaaaaagc tatgactgga ccgctattat
     4441 ggctatgcct tatctggaag gtgtcgcaga aaaatcggct gaccaatggt taatacaatt
     4501 gaccaatcaa attaaaaaca tccctcaggc taaagacaaa tctattttag aattacaggc
     4561 acaaaactgg cagaaaaatg gtcagcatca ggctatttct tcgcaacaac tcgctcactg
     4621 gatgagccta ttacaactga atggagtgaa aaactatggt tattatcccg acaattttct
     4681 gcataaccaa cctgaaatag accttattcg tcctgagttt tcaacagcct ggtatccgaa
     4741 aaatgattaa tcgcatcgta tcgtttttta tattatgtct ggtgttatgc atacccctat
     4801 gcgtagcgta ctttcactct ggtgaactga tgatgaggtt cgttttcttc tggccgtttt
     4861 ttatgtccat tatgtggatt gttggcggcg tctatttctg ggtctatcgt gaacgccact
     4921 ggccgtgggg agaaaacgca ccagctcccc agttgaaaga taatccgtct atctccatta
     4981 tcattccctg ttttaatgag gagaaaaacg ttgaggaaac catacacgcc gctttagcac
     5041 agcgttatga gaacattgaa gttattgccg taaatgacgg ttcaacagat aaaacccgtg
     5101 ccatcctgga tcgcatggct gcacaaattc cccatttgcg ggtcattcat ctggcgcaaa
     5161 accaggggaa agccattgcg cttaaaaccg gagctgccgc ggcgaaaagt gaatatctgg
     5221 tgtgcattga tggcgatgcg ttattagacc gcgatgcggc ggcatatatt gtggaaccga
     5281 tgttgtacaa cccgcgtgtg ggtgccgtaa ccggtaatcc tcgtattcga acacgttcta
     5341 ccctggtggg taaaattcag gttggcgagt attcctcaat tattggtttg atcaagcgaa
     5401 cccagcgtat ctatggaaac gtatttaccg tttccggtgt tattgccgca tttcgtcgca
     5461 gcgccctggc agaagtgggt tactggagtg acgatatgat caccgaagat attgatatta
     5521 gctggaagct gcagttgaat cagtggacga ttttttacga gccacgggcg ctgtgctgga
     5581 tattaatgcc tgaaacgtta aaagggctgt ggaaacagcg cctgcgctgg gctcagggcg
     5641 gtgcagaagt attcctcaaa aatatgacaa ggttgtggcg caaagaaaac tttcgaatgt
     5701 ggccgctgtt ttttgaatac tgcctgacga caatatgggc cttcacctgc ctggtcggtt
     5761 tcattattta cgcagtccaa cttgccggtg taccgttaaa tattgaattg acacatatcg
     5821 ctgcgacaca tactgccgga atattattgt gtacgttatg tttactgcaa tttattgtca
     5881 gcctgatgat cgagaatcgc tatgagcata atctgacttc atcgcttttc tggattattt
     5941 ggttcccggt tattttctgg atgctgagcc tggcaacgac attggtatca tttacacgag
     6001 tcatgttgat gcctaaaaag caacgcgccc gttgggtaag tcccgatcgc gggattctga
     6061 gaggttaata tgaacaattt aattattacg acccgacaat caccagtacg tttactggtt
     6121 gattatgttg ccacaaccat cttgtggaca ttatttgcgt tgttcatatt cttattcgcc
     6181 atggatctgc tgacgggtta ttactgacaa agcgaggcca gaagccgact tcagttctat
     6241 tttttgctgg cagtggcgaa tgccgtcgtg ttaattgtct gggcgctgta caataagctg
     6301 cgttttcaaa aacagcagca tcatgcagcc taccaatata cgccgcaaga atatgcagag
     6361 agcttagcaa tacctgatga gctctatcag caactacaaa aaagccacag gatgagcgta
     6421 cacttcacca gccaggggca aataaaaatg gttgtttcag aaaaagcgct agtccgggca
     6481 taaacaccca aaacaaagcc cggttcgccc gggctctgca ccgataatac acttaactgt
     6541 atgcatgcag cgtacgttgg caaagtgccg aacgtacgca gtcctcttta ccgaaccgga
     6601 cgatcccaac catttcatct tcttcgaaac gttccagcgc gtcacttaat ccggagcaca
     6661 cgccgcgagg caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc
     6721 cgaggcgggt taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa
     6781 tgacgactgc attttcaaag gtacgtccac gcatataggc gaacggcgca atttccacct
     6841 tccctatttc cggtcgcagg cagtactgca taaaggaagc ccctaagcgc cggaccagca
     6901 cgtcgtagac cgggcgaaaa tagggagcaa acttttctgc gatatctcca ggtaagaagc
     6961 caagatcttc atcggcttgc agaactggac gggtgacgat aatcctgtcg acatccttat
     7021 gtatcagggc ctctgccgct tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc
     7081 ccgtggcgaa tatcaactgc ttactctcaa tagccttcag atagtgcaat tgcgcttcat
     7141 ttcgcgcgag gatgggcgaa gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc
     7201 cgcccatctg cacaagcgag gtgacctatt cttcttcacg ctgcttatgg ctgcgcgaat
     7261 cccgtctcag cacacgtttt gcctcacgac gagctttgat cactgctttt tgtcttccca
     7321 tggagagcac cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg
     7381 cacgaacatc agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg
     7441 aaaaacggct acgcacaccg tttgcggcgt cggtaacacg agtaaagaga ggaagtgaga
     7501 aaaatacaga gtgacgaagt tgctaccgga ggagaaatct ccgcaagtgg tggagcgtag
     7561 cttaacaaga cattgtccag aacaggacgt taccatccgc gatctccata gtgactgact
     7621 atcactgccg ggaacttccg ctgctactta ataattacaa cagatctcgc attcattgca
     7681 acaatatatt cacttatatt taaccataaa acaccatttc agtgacatta gtttctactg
     7741 gaaagatgac agagtgatga cagatatgaa aaaagctgtg tgctttcagc aggattcgca
     7801 tcaggcatct gcgcacgaac gccggatgcg gcgtgaacgc cttatccgcc taccgaaccc
     7861 gcaccgattg taggcataag aagacgcgca agcatcgcat caggcatctg cgcaagaacg
     7921 ccttatccgt ctataaacat taaacccgca ataacgcgct tccgagataa tcgttcgcgt
     7981 ccttcactcc cggcagcgca aaaaaataac cgccgccgat aggtttaacg tattcctcca
     8041 gcgcttcgcc attgagcctt ttttgtacag tcaggaagcc tttttccaga tcgtgttggt
     8101 aacagacaaa cagtagcccc atatccagtt gcccggagtt ggtgacgccc agtgaataac
     8161 tgtagccacg acgcagcatc aggctggact cactctccgc cgtgcgggga ttcgccagcc
     8221 ggatatggct gtccagcgca atcaccttcc cttccggatc gctggcgtaa tcgggaacat
     8281 catgctcatg ctgcattccc agcggcgcac cggtttgctt atcacgacca aaaatcgtct
     8341 gctgttcttt cagcggagtt ctgtcccaaa attccactcg aaactgaatc aagcgtactg
     8401 cctgatagct gccaccgatt gtccacgctg gctcctgctg atctgccgtt acccacacca
     8461 ctttttgcat caacttatca ttctggctat cgggattggc agtgccgtct ttgaaaccca
     8521 gcaaattaat cggcgtctct ttgcctttac tacgcgccgc gtgatcggaa ataaaccctt
     8581 cccgcttcca gcgcacgctg agcaaatctg gcgtatgttt gatgatatcg cgcagcgcat
     8641 ggatcaccgt gtcctgggtg ttggcgcaaa tttgtagcaa cacatcacca tgacataacg
     8701 ccgcatccag cgagtcgttg ggaaaacgcg tcatcttctg caactttttc ggcatctgtg
     8761 gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac cgataacgtg atggtgagat
     8821 tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag tggtggcagg cgcggatttg
     8881 gcgtttctgg tgccgctccg ccctgagtca gaaaagcaaa acgctgagtc aacaagcgaa
     8941 acaaccgttc aagatcgggt ttatcgctgg caagcacatc aaacgccacc agcatcattg
     9001 cggcctgttg tggcgtcagg atccctgcct gatgctcacc ataaaacggc tgtttctcat
     9061 tgcgtgcatc cggtgaaagc gtacccggcg cactttgcgt tttttgtgca tgagcgaccg
     9121 gacaacttcc cgccagcgcc agcgcaccaa tgcctttcag taaacgtcgg cgtgacggtt
     9181 cattcacgcc gtttttatct tcatactgca taatgcttaa tccaatccca gcacaccgcg
     9241 aagttgcgcc agatcttccg ccagcgcagt aatcggtcct ttcagtgcat tccggtcggc
     9301 atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta cggtatttcg ccagaatggt
     9361 atcgaccttt ttaaagttgg catcgacttt tgccaacagt tccgggttgg ctttttgcag
     9421 ttgtggacgc agcagatcga caattttctg cgagccttca acgttagcct ggaaatccca
     9481 cagatcggtg tggctgtagc gatcttcttc accgctgatt ttgctggctg ccacttcctc
     9541 aatcagtccg gctgcaccgc cgaccacttt tgaaggtggg aaagccagtt cactgatgcg
     9601 tttttgcaaa tcgaccacat cggtataaag ctggtcagcg tactgatcca tgcctttggt
     9661 ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa ccggtgaatt tcggatcggc
     9721 ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg ccatccagat cggagaacag
     9781 ttcagcaatc ggttcgatgc gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc
     9841 tttttcgata tcgcctgctt taatagcgtc agtaaaggct ttcgtgtcgg tcaccagctg
     9901 cgtggtttcc gccatgacat acgctttata tgcagtaatt gcaccaccaa gacttaacag
     9961 cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg acgatcaact tccctttcgg
    10021 gttagtcagc agaccgcagg tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt
    10081 ctggctaaag ccaggggcga tattttcccg ctcttccacc accatcacgc ctttgaggat
    10141 ctcccactcc agcgccttct ggctgtggtt ctgaataatg aactgtgttt tcccggcgtt
    10201 aaccgtaatg gtcatcggtt cgcactgctt atcggtcacg gtcaccttga cctgcggcac
    10261 attagcggcg ttagccataa aagcagaaga gaacagcgca gccacgctca actgcaatgc
    10321 gttacggcgg aagttaatgg tcatgacgag actatccctt taaagtatgt tgtaactaag
    10381 caagagtttg cgtcgtattt gctacgcgga gcgagacgct gtcgcccctg cgcgtggtgg
    10441 cagggcaaat gccaccagcg ccgggatgag ataaataaac cagacggcga cttcgctgac
    10501 gctcggtgct tcctgatagc caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt
    10561 tgagaggacc gcgctcatat cgaaggcgat ttcctgaaag tggttccaca atccggcttc
    10621 atgaaatgcg cgaatggcac cagccgccag ccctgcggcg acgaagagaa taaacaggct
    10681 ggtccattta aaaaatgcac caagattgag gcgaataccg ccccagtaga gcaggaagcc
    10741 gagcaccacg gcagtagcaa gaccgagcat tgcacccagc ggcggccaga tcccgacatc
    10801 ttgttgaaat gccgccagca ggaaaaagac cgactccagc ccttcccttg caacggcaaa
    10861 aaagaccatc atcaccagcg cccagccatg atgatttcca cgctgcaatg cgctatcgac
    10921 tgcctgttcc agttgcactt tgacgttgcg cgacactttg cgcatccaga aaaccatcca
    10981 ggtaaggatc accacggcga tcaccgccac gataccttca aacagttcct gttctttttg
    11041 cggaaattcg ccggtggttt cgttaatgaa gatacccagg cccaggcaca acgcagcggc
    11101 aagcaacacg ccaatccaca tcacaccaat ccatcggcct cgctgggtac gcttaaggta
    11161 gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct tcgcgcaaca taatgagaaa
    11221 cggaacaaac atgccaccca cccttaaacg tttttctgag tcaatctatg caaagaaaag
    11281 taaatcgcag tgatagcgat tatcattacg gtaaagaaaa atacaagcgg aatgtttata
    11341 aaatgatgtt ggaatgtaaa caaagagagg taatgccaag ggatataaac gattattcca
    11401 gggtagaaag atgggattgt ttgcaaagaa ccatgtgttc ttgccggatg ccgcgtaaac
    11461 gccgtatcca gccgacaaaa ctgtccagac tcaatgcatc agagcttcca tgtaaaactg
    11521 ataagcgggt gtatcaagca atttcgtcca tccgcgatta tcaaatctaa ctttttgccc
    11581 ggaacataat aacagctcgg gtgttggtgg cgtcaaaatc gctgaagcgt tccccgaagg
    11641 ccgggacagc agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa
    11701 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
    11761 gcaggctaag tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
    11821 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac
    11881 actacaatcc caatactgcc gaagataaag cccggaataa tttcgtagag tcccagccag
    11941 ccgaactgct tccagacgat aaccgtcagc gcaccgatga tcatccccgc cagcgcaccg
    12001 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
    12061 aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc
    12121 gcaatcgcca ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc
    12181 tggctggcat gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac
    12241 accagcagct ggcagcttaa cgttgacatt accgccgcca gaattgccga cagcagaatc
    12301 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacacg ttcagcgttc
    12361 tggtttaccg cacctgctac cgccgggtgc tcattaaagt aagcaatccc aaagaagccg
    12421 acagccaccg cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg
    12481 acaatgctgt ggtgagaatc cgccgccata aaacgcgcca gaatatgagg ctgaccgaag
    12541 taacccagcc cccaacccat caatgagata atggcaacaa agttcagccc tttgagcata
    12601 tccacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
    12661 ctgataatga cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
    12721 gtatcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca
    12781 gctcccgccc acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg
    12841 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag
    12901 ataatgcgca aaatgcggct tttatcttca aagcgcccgg tgaaataatc cggcagggtt
    12961 aaggcgttat tgttgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg
    13021 atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa
    13081 accgcgcccg gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
    13141 gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt
    13201 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
    13261 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
    13321 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
    13381 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
    13441 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
    13501 cacctttcaa aaatgttaac tgccgcagag aaaaagtctg atttattttt ttaatccctg
    13561 tcatatcgat ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat
    13621 ttaacatggt tgcacaaagt tgcaacatca tggatatttc acgataacgt taagttgcac
    13681 ctttcagaac aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga
    13741 cgcgacgcgt gagcgtatta agtctgccgc gacacgtatc gatcgcacac cacactggtt
    13801 aattaagcag gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga
    13861 gctacctgcg ctgctttctg gcgctgccaa tgagagcgat gaagcaccga ctccggcaga
    13921 ggaaccacac cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg
    13981 cgccgcgatc accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga
    14041 acaagcccgc ctgccgcagc cagttgctgc acaggcgcac aaactggcgt atcaactggc
    14101 cgataaactg cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aggggttatt
    14161 gcaggagttt tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cagaagcgtt
    14221 gttgcgtatt cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaacgg
    14281 taactggcag tcacacattg gtcgtagccc gtcactgttt gttaatgccg ccacctgggg
    14341 gctgctgttt actggcaaac tggtttccac ccataacgaa gccatcctct cccgctcgct
    14401 gaaccgcatt atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat
    14461 gcgcctgatg ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc
    14521 ccgcaagctg gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact
    14581 gactgccgca gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccatcgg
    14641 taaagcgtct aacggtcgtg gcatctatga agggccgggc atttcaatca aactgtcggc
    14701 gctgcatccg cgttatagcc gcgcccagta tgaccgggta atggaagaac tttacccgcg
    14761 tctgaaatca ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga
    14821 agaggccgat cgtctggaga tctccctcga tctgctggaa aaactctgtt tcgagccgga
    14881 actggcaggc tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt
    14941 ggtgattgat tacctgattg atctcgccac ccgtagccgt cgccgtctga tgattcgtct
    15001 ggtgaaaggc gcgtactggg atagtgaaat taagcgtgcg cagatggacg gccttgaagg
    15061 ttatccggtt tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa
    15121 actgctggcg gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct
    15181 ggcagcgatt tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg
    15241 cctgcatggt atgggcgagc cactgtacga gcaggtcacc gggaaagttg ccgacggtaa
    15301 acttaaccga ccgtgtcgta tttatgcccc ggttggcaca catgaaacgc tgctggcgta
    15361 tctggcgcgt cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga
    15421 cacctctttg ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc
    15481 gcaacaggaa gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta
    15541 cggtcacggg cgcgacaact cggcagggct ggatctcgct aacgaacacc ggctggcctc
    15601 gctctcttct gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga
    15661 acaaccggta gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat
    15721 tgtgggcttt gtgcgtgaag ccacgccgcg tgaggtagaa caggcgctgg aaagtgcggt
    15781 taataacgcg ccaatctggt ttgccactcc tccggttgaa cgcgcggcga ttttgcatcg
    15841 cgctgccgtg ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc
    15901 cggaaaaacc ttcagtaacg ccattgccga agtgcgcgaa gcggtcgatt ttctccacta
    15961 ctatgccgga caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt
    16021 ggtgtgtatc agtccgtgga acttcccgct ggctattttc accgggcaga ttgctgccgc
    16081 actggcggca ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc
    16141 gcaagggatc gccattttgc tggaagcggg tgtaccgcca ggcgtggtgc agttgctgcc
    16201 aggtcagggg gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtac gcggggtgat
    16261 gtttaccggt tcaaccgaag tcgctacgtt actgcagcgc aatatcgcca gccgcctgga
    16321 cgctcagggt cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt
    16381 cgattcttca gcactgaccg aacaggtcgt cgtggatgta ctggcctcgg cgttcgacag
    16441 tgcgggtcag cgttgttcgg cgctgcgcgt gctgtgcctg caagatgaga ttgccgacca
    16501 cacgttgaaa atgctgcgcg gcgcaatggc cgaatgccgg atgggtaatc cgggtcgcct
    16561 gaccaccgat atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat
    16621 tcagaccatg cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga
    16681 tgcccgtgaa tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt
    16741 tgccgaattg caaaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa
    16801 ccagctacca gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt
    16861 ccatacgcgt attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct
    16921 gtatgttaac cgtaatatgg tgggcgcagt ggtgggtgtg cagccattcg gcggcgaagg
    16981 gttgtccggt accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa
    17041 tcgcccggaa agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga
    17101 tgcacagttg aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa
    17161 tcgtccagaa ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaacaca
    17221 acgattgctg ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg
    17281 cgtgttgtgt attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc
    17341 ggtgggcagc caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt
    17401 gccatcggca gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc
    17461 ctttgatgcg gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt
    17521 tgctgcgcgg gatggcgcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat
    17581 ccttctggaa cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg
    17641 taacgccagt ttaatgacta taggttaaac aggtagccgg aggatgttac aacctcctcc
    17701 ggcatcttta acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt
    17761 gattgaaaaa tatctcatcg cgcaacgtcg cgcctgtcac cgcctccacc tgaggggcga
    17821 aatcggcgta atgttgagtg gaagcccaaa tcatgaaaat caaatgctgc ggatcaatcg
    17881 gcgcgagttt gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg
    17941 ccttcaaatc gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac
    18001 agaacaggcg cgaagcctgc ggataatcgc gggagacttc cagcttcaga cggatgtact
    18061 cttttatcgc cgccagcgga gcgaaatctt cacgaaacgc ttttaacggt gccagccaga
    18121 tatcgagaat ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca
    18181 gcagattggt ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa
    18241 aaccgaattg tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta
    18301 ctgcgcgcga acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct
    18361 tgtagattgg caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc
    18421 gcgcacagat gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga
    18481 tattttagat aacgcattgt taacatatta acaacattaa ctttttaaaa actggcatcc
    18541 gctttgcaaa caagccatta cgcggctgaa aaggatatgc aggatgcagc gccccgtctg
    18601 actttcactt tacgagacga agagaggttg atgatgaaaa ttggcgtatt cgtacctatt
    18661 ggcaacaacg gctggctcat ttcgacccac gcgccgcagt acatgccgac ctttgaactg
    18721 aataaagcca ttgtgcaaaa agcggagcac taccatttcg atttcgccct gtcgatgatc
    18781 aaactgcgtg gctttggcgg caaaactgag ttctgggatc acaaccttga gtcgttcacc
    18841 ttgatggcgg ggctggcggc cgtcacctcg cgcattcaga tttacgccac tgctgccacc
    18901 ttaacgttac ctccggcaat cgtcgcccgt atggccgcaa ccatcgactc catctctggc
    18961 gggcgttttg gcgtcaacct cgtgactggc tggcaaaagc ccgagtatga gcagatgggc
    19021 atctggcctg gcgatgacta tttctcccgt cgttacgact atctcaccga atatgttcag
    19081 gtgctgcgcg acctgtgggg cacggggaaa agcgatttta aaggcgattt tttcaccatg
    19141 aatgattgtc gcgtcagtcc gcaaccgagt gtccccatga aagtgatctg cgccgggcaa
    19201 agcgacgctg gcatggcgtt ctccgcccag tatgccgatt tcaacttctg tttcggcaaa
    19261 ggcgtaaata cgcccacggc tttcgccccg accgctgcgc ggatgaaaca ggccgcagag
    19321 caaaccgggc gcgacgttgg ttcttatgtg ttgtttatgg tgattgccga tgaaaccgac
    19381 gatgccgctc gcgccaaatg ggaacactac aaagcgggcg cggatgaaga ggcgttaagc
    19441 tggctaaccg aacaaagtca gaaagatacc cgctccggta ctgataccaa cgtccgtcag
    19501 atggccgatc ccacttcggc ggtaaacatc aatatgggga cgttagtcgg ttcttacgcc
    19561 agtgtcgcgc gcatgttaga tgaagtcgca agcgtgcctg gtgccgaagg cgtgctgttg
    19621 accttcgatg attttctgtc gggaatcgaa accttcggcg agcgcattca accactgatg
    19681 cagtgccgcg cccatctacc tgcgctgact caggaggtgg catgatgaca accttaaccg
    19741 ctcgaccgga agccattacc ttcgatccgc agcaaagtgc gctgatcgtg gtggatatgc
    19801 aaaacgccta tgccacgcca ggcggctact tagatctcgc cgggtttgat gtctcaacca
    19861 ctcgcccggt cattgccaac attcaaaccg ctgtgaccgc agcgcgagcg gcagggatgt
    19921 tgatcatctg gtttcaaaat ggctgggatg aacagtatgt cgaggctggc ggccccggct
    19981 caccgaattt tcataaatcg aacgccctga aaaccatgcg taatcagccg cagctgcagg
    20041 ggaaattgct ggcgaaaggc tcctgggatt atcaactggt ggatgaactg gtgccgcagc
    20101 ctggcgatat tgtgctgccg aagccgcgct acagcggttt cttcaataca ccgctggaca
    20161 gcattttgcg cagccgtgga atacgccatc tggttttcac cggtatcgct accaacgtct
    20221 gcgtcgaatc gacgctacgc gatggctttt ttctggagta tttcggcgtg gtgctggaag
    20281 acgcaacaca ccaggcgggg ccggaatttg cacagaaagc cgcgttgttc aatatcgaaa
    20341 ccttttttgg ctgggtcagc gacgtcgaaa cgttctgcga cgcgctttct cccacatcct
    20401 ttgctcgtat cgcttaagga gtttaacgat gccaaaatcc gtaattattc ccgctggcag
    20461 cagcgcaccg ctggccccct tcgttcccgg cacgctggct gatggcgtgg tgtatgtctc
    20521 cggtacgctg gcttttgatc aacataataa cgtgctgttt gccgatgacc caaaggcgca
    20581 aacccgccac gttctggaaa ctatccgcaa ggtgatcgag acggcgggtg gcacgatggc
    20641 ggatgtgacc ttcaacagca tctttattac cgactggaaa aattacgccg cgattaacga
    20701 aatctacgcc gagttttttc cgggtgataa accggcgcga ttctgcattc agtgcggact
    20761 ggtaaaacct gacgcgctgg tggaaatcgc cacaattgcg catatcgcca agtgaggccg
    20821 cgatgaaact ttcactctca cctccccctt atgctgatgc gcccgtagtg gtgttgattt
    20881 cgggtcttgg cggtagcggc agttactggt tacagcaact ggcggtgctg gagcaggagt
    20941 atcaggtagt ctgttacgac cagcgcggca ccggcaataa tcccgacaca ctggcagaag
    21001 attacagtat cgcccagatg gcagcggaac tgcatcaggc gctggtagcc gcagggattg
    21061 agcattacgc ggtggtcggc catgcgctcg gtgcgctggt gggaatgcag ctggcgctgg
    21121 attatcccgc gtcggtaact gtgctgatca gcgttaacgg ctggctacga ataaacgccc
    21181 atacgcgccg ctgttttcag gttcgcgaac gattactgta tagcggcggc gcgcaggcat
    21241 gggtggaagc gcagccgttg ttcctctatc ccgccgactg gatggcggcc cgcgcacctc
    21301 gccttgaggc agaagacgcg ctggcactgg cgcattttca gggcaaaaat aatttactgc
    21361 gtcgacttaa cgccctcaaa cgcgctgact ttagtcacca tgcggatcgc atccgctgcc
    21421 cggtgcaaat catctgcgcc agtgatgatc tgctggtgcc aacagcatgt tccagtgaac
    21481 ttcatgccgc cctgcccgat agccagaaaa tggtgatgcc ctatggcgga cacgcctgca
    21541 acgtgaccga tcccgaaacg tttaatgctc tgttactcaa cgggcttgcc agcctgttac
    21601 atcaccgtga agccgccctg taaggaattg ctatgaacga agccgttagc ccaggtgcgc
    21661 ttagcaccct gttcaccgat gcccgcactc acaacggctg gcgggagaca cccgtcagcg
    21721 atgagacgtt acgggagctt tatgccctga tgaaatgggg gccgacatca gctaactgtt
    21781 ctccggcacg gatcgtgttt atccgcactg cagaaggaaa agaacgtctg cgcccggcgc
    21841 tttccagcgg caatctacaa aaaaccctga ccgcgcccgt caccgctatc gtcgcctggg
    21901 acagtgaatt ttatgaacgg ttaccactac tgtttcccca cagtgatgcc cgcagttggt
    21961 ttacctccag cccacaactt gccgaagaaa cagcgtttcg caacagttcc atgcaggcgg
    22021 cctatctgat cgtcgcttgc cgtgcgctgg gactggatac cggcccgatg tcgggttttg
    22081 accgtcaaca cgttgacgac gccttttttg cgggcagcac gctgaagagc aatctgctga
    22141 ttaatatcgg ctatggcgat agcagcaaac tttttgcgcg cctgccacgt ctgtcctttg
    22201 aagaagcctg cgggctgttg taaggagcaa actatgaaca tagttgatca acaaaccttt
    22261 cgcgatgcga tgtcctgtat gggcgcggcg gtcaatatca tcaccacgga cggtccagcc
    22321 gggcgcgccg ggttcaccgc cagcgccgtc tgcagcgtga ccgatacgcc gcccacatta
    22381 ctggtgtgcc tgaatcgtgg ggcgtccgtc tggccggtat tcaatgaaaa tcgaacgctg
    22441 tgtgtaaata cacttagcgc cgggcaggag ccgctttcca acctttttgg cggcaaaacg
    22501 cccatggaac accgctttgc cgccgcccgc tggcagacgg gtgtgaccgg atgcccgcaa
    22561 ctggaagagg ccctggtttc gtttgactgc cgtatcagcc aggtggtgag cgtcggcacc
    22621 cacgacattc tgttttgcgc catcgaagcg attcatcgtc acgccacacc ctacgggctg
    22681 gtgtggttcg atcgcagtta tcacgcgctg atgcgccctg cttgttaacc tccttaagga
    22741 gacagctgat ggcaatgttc ggttttcctc actggcagtt aaaatcgacc tctacagaga
    22801 gcggcgtggt cgcgccggat gaacggttgc cgtttgctca gacggcagta atgggcgttc
    22861 aacacgcggt ggcgatgttt ggcgcgacgg tgttaatgcc gattctgatg gggctggatc
    22921 ccaatctttc cattttaatg tcggggatcg gcacgttact gtttttcttt attaccggcg
    22981 ggcgcgtccc cagttatctc ggctccagcg cggcttttgt cggcgtggtg atcgccgcga
    23041 cgggctttaa cggtcagggc atgaacccga atatcagcat cgcgctgggc gggattatcg
    23101 cctgtgggct ggtttatacc gtcattggcc tggtggtgat gaaaatcggt acacgctgga
    23161 ttgaacggct aatgccgccg gtggtgacgg gtgcggtggt gatggcgatt ggcctgaact
    23221 tagcgcctat cgcagtgaaa agtgtatcgg cttcggcctt tgatagctgg atggcggtaa
    23281 tgacggtgct gtgtattggg ctggtggcgg tgtttacccg aggcatgatc cagcggttat
    23341 tgattctcgt aggattaatt gtcgcctgtc tgctgtatgg cgtgatgacc aacgttttag
    23401 ggctgggaaa agccgtcgat tttactctcg tcagccatgc tgcctggttc ggtttgccac
    23461 atttttccac tcctgctttt aatggtcagg caatgatgct gattgcgccc gtggcggtga
    23521 ttctggtggc ggaaaactta ggtcatctca aggcggtcgc cgggatgacc ggacgcaata
    23581 tggacccgta tatggggcga gcattcgtcg gtgatggtct ggcaacgatg ctttccggct
    23641 ctgtcggcgg tagcggcgtc accacctatg ctgaaaatat cggtgtgatg gccgtgacaa
    23701 aagtctactc gacgctggtg tttgttgctg ctgccgtcat cgccatgctg ttgggctttt
    23761 cgccgaaatt tggcgcgctg atccatacca ttccggccgc ggtgattggt ggtgcatcaa
    23821 ttgttgtgtt cgggctgatt gccgtcgcag gcgcaaggat atgggtacaa aaccgtgtcg
    23881 atttaagcca gaacggtaat ttgattatgg tcgcagtaac cttagtgctg ggcgcgggtg
    23941 attttgctct cacgctgggc ggttttacgt tgggagggat tggtacagca acctttggcg
    24001 cgattttact caatgcgttg ttaagcagaa agttggttga cgttccaccg ccagaagttg
    24061 ttcatcagga gccataaaca gaaaaccgcc gggattaccg gcggtgatga ctttttgacg
    24121 agcctaagaa aaaaatgcat tcgtcattga ttagcccggc tagttgtcgc ttttgccgtg
    24181 actgctcttg ccaccttttt tacctgcttc agatgcgcgc tgcgggtcat tcttgaaatt
    24241 acccccgctg tgctgtccac cttttttacc tgcttctgat gctctttcgc ggtcttctgc
    24301 aaaattgccg gaaccgcctc gatggtttgc cattactgac ctccgtctgg gtatagacat
    24361 catattgcat agaattaacg cggcgtatgg cggctgccat attttcgcta cgtgggacta
    24421 agcttagtga actgacgcgg gcagacccgc gtttggtaat atcctgcaac agcttcttag
    24481 cgagaataga tatcatctca aataattatc cataagccgc tcttatgatt aaaaaaacgc
    24541 gatgagataa aaatgtgtca ttttgcgaca aaattacgtg cttgtgaaag ttgttataaa
    24601 tcaaataagt ggttgtgaaa tttgcactct gaaaaggacg tcttatcttt aaataagtgg
    24661 tagcgaatcg ctacggaata gagataacac gaggagtggt tagaaatggc taaagttctg
    24721 gtgctttatt attccatgta cggacatatt gaaacgatgg cacgcgcagt cgctgagggt
    24781 gcaagcaaag tcgatggcgc agaagttgtc gttaagcgtg taccggaaac catgccgccg
    24841 caattatttg aaaaagcagg cggtaaaacg caaactgcac cgattgcaac cccgcaagaa
    24901 ctggccgatt acgacgccat tatttttggt acacctaccc gctttggcaa catgtccggt
    24961 caaatgcgta ccttcctcga ccagacgggc ggcctgtggg cttccggcgc actatacgga
    25021 aaactggcga gcgtctttag ttccaccggt actggcggcg gtcaggaaca aactattact
    25081 tcaacctgga cgacccttgc gcatcacggc atggtaattg tccccattgg ctacgcagcg
    25141 caggaattat ttgacgtttc acaggttcgc ggcggtacgc cgtacggcgc aaccaccatc
    25201 gcaggcggtg acggctcacg ccagccaagc caggaagaac tgtctattgc tcgttatcaa
    25261 ggggaatatg tcgcaggtct ggcagttaaa cttaacggct aatcttcaac aggaggatac
    25321 gcatgccaac tcaagaagcg aaagctcatc acgtcggtga atgggcatct ctgcgcaata
    25381 cgtcgccgga aatagccgag gccattttcg aagtcgccgg gtacgacgaa aaaatggcgg
    25441 aaaagatttg ggaagaaggt agcgatgaag tgttagtcaa agcctttgct aagaccgata
    25501 aagattcgct tttttggggc gaacagacca tcgaacgtaa aaacgtttaa ccagcgactc
    25561 ccccgcttct cgcgggggag ttttctgtta tttcaccgct tcattcaaca cgctatcaaa
    25621 tttatccatc gggcagaaac cgttggcatc tatcgggcaa ccgcttaatt ccagcgtcac
    25681 acgctgagca ggtgcctgca gggttaacgc atcggcatta cgtaactgtt ccgcactctg
    25741 atacacatat tcaattttca tcaaatcgcg attggctttg ctgtcatgcc aacgctggaa
    25801 aacgattttg ccgccaatcg gcgtgcgttc gttctggtca tgcaactgat acggtttgaa
    25861 atccagcgcc gttaacagag aggcaatgtt ggagtcgtgc ccaaccaaca ctgtaatttt
    25921 cggtgcgctg gtgcgatcgg tgaccagagc tttgtcgata taactgacca gcggtttcgc
    25981 aacattgcgc gccacttccg gtgaggtaaa caggctgtcc tggtagccgt ttttaagctt
    26041 cgacaacacc ttccactgct ggtcagattt gatttctccc caggccacct gatccatcgg
    26101 aaaaccttcg taatattgca aagtaaacgc atctaccagc gagttgccga ctttcagcgg
    26161 cccggaaaca cctggttctt gttgatactt ggcgctaaag gtatttttgc catccaccag
    26221 cgaacactgt tgtttctctt tacaggcagg ggaatcttta tagttaacga ttttttccag
    26281 tagctggtag ctgtcggtaa gctggagttt gctgagctct ttctccattg ccgccaccgc
    26341 ctgttcactg aatgcggcgg aatcatcggt gatcaccggg ttaaaggttg ggtccatagt
    26401 gcccattttt tcctggtgat gcacaggaat atcacacccc gggaatgcgc cggtaataaa
    26461 gaactgtgcg gtcgcaacgg tacgttgcag actattggca taggcataaa cggtgtacgg
    26521 cggcgggcat tcccccgatt tcaccatccc ctgctctgcc agccattcac gcatgtaatg
    26581 gcccatatac acttcgagca cgccaccttt ggtggtgagt tgcccaccgg ggacgtccca
    26641 ttctggccat ttattcggcg tcgactgctc cagcacactg ccattgttcg ccagcggcgc
    26701 acgtaagtta tggcggctca tcatgagcac ttgctgtagc tgatagcctt ccggtacggt
    26761 ttgtgcctga gcgtttgaag cgagtaaaac tatccctgcc acagctgcgg cgattagcgt
    26821 tttgttcatt cctggcacct cttttgttat caatcactaa agagtgtgac agaaatatga
    26881 cttttttccg ggaacaggtt cgcaaaatgg cttgttgaac gcctgttttt ctatatacat
    26941 cttcaaaaac aggcaaggtt tgacgcaaac tatgcctgat tagcccctgc ctcgcgtacg
    27001 ccgctgataa ttgcacctgt ggctaaacag aatatctgcg gcgttaccaa tacagagaca
    27061 atatttacca ataaaaaagt gtgagactaa taacaatggt ttttagcata tgcctttgcc
    27121 attaaattgc ataacgattt aaatagtcga gttgcgcatt catttcctta ttttttaact
    27181 tgtaagcctg agcaaaaagt tcagg
//
LOCUS       DAZFWP020000059        26888 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFWP020000059 DAZFWP020000000
VERSION     DAZFWP020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.059"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            88..537
                     /gene="alaE"
                     /locus_tag="ACHK0Z_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             88..537
                     /gene="alaE"
                     /locus_tag="ACHK0Z_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU4502295.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(574..918)
                     /gene="ygaC"
                     /locus_tag="ACHK0Z_RS11410"
                     /old_locus_tag="pgaptmp_002282"
     CDS             complement(574..918)
                     /gene="ygaC"
                     /locus_tag="ACHK0Z_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU4502296.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1070..1399
                     /gene="ygaM"
                     /locus_tag="ACHK0Z_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             1070..1399
                     /gene="ygaM"
                     /locus_tag="ACHK0Z_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU4502297.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1647..1892
                     /gene="nrdH"
                     /locus_tag="ACHK0Z_RS11420"
                     /old_locus_tag="pgaptmp_002284"
     CDS             1647..1892
                     /gene="nrdH"
                     /locus_tag="ACHK0Z_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU4502298.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1889..2299
                     /gene="nrdI"
                     /locus_tag="ACHK0Z_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             1889..2299
                     /gene="nrdI"
                     /locus_tag="ACHK0Z_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU4502299.1"
                     /translation="MSQLVYFSSNSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2272..4416
                     /gene="nrdE"
                     /locus_tag="ACHK0Z_RS11430"
                     /old_locus_tag="pgaptmp_002286"
     CDS             2272..4416
                     /gene="nrdE"
                     /locus_tag="ACHK0Z_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4502300.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4426..5385
                     /gene="nrdF"
                     /locus_tag="ACHK0Z_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             4426..5385
                     /gene="nrdF"
                     /locus_tag="ACHK0Z_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4502301.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5740..6942
                     /gene="proV"
                     /locus_tag="ACHK0Z_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             5740..6942
                     /gene="proV"
                     /locus_tag="ACHK0Z_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU4502302.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6935..7999
                     /gene="proW"
                     /locus_tag="ACHK0Z_RS11445"
                     /old_locus_tag="pgaptmp_002289"
     CDS             6935..7999
                     /gene="proW"
                     /locus_tag="ACHK0Z_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU4502303.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8057..9049
                     /gene="proX"
                     /locus_tag="ACHK0Z_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             8057..9049
                     /gene="proX"
                     /locus_tag="ACHK0Z_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU4502304.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9341..10525
                     /locus_tag="ACHK0Z_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             9341..10525
                     /locus_tag="ACHK0Z_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502305.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10649..11386
                     /gene="ygaZ"
                     /locus_tag="ACHK0Z_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             10649..11386
                     /gene="ygaZ"
                     /locus_tag="ACHK0Z_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU4502306.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11376..11711
                     /gene="ygaH"
                     /locus_tag="ACHK0Z_RS11465"
                     /old_locus_tag="pgaptmp_002293"
     CDS             11376..11711
                     /gene="ygaH"
                     /locus_tag="ACHK0Z_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU4502307.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLG
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11802..12332
                     /gene="emrR"
                     /locus_tag="ACHK0Z_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             11802..12332
                     /gene="emrR"
                     /locus_tag="ACHK0Z_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU4502308.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHLLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12459..13631
                     /gene="emrA"
                     /locus_tag="ACHK0Z_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             12459..13631
                     /gene="emrA"
                     /locus_tag="ACHK0Z_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU4502309.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13648..15186
                     /gene="emrB"
                     /locus_tag="ACHK0Z_RS11480"
                     /old_locus_tag="pgaptmp_002296"
     CDS             13648..15186
                     /gene="emrB"
                     /locus_tag="ACHK0Z_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU4502310.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15250..15765)
                     /gene="luxS"
                     /locus_tag="ACHK0Z_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             complement(15250..15765)
                     /gene="luxS"
                     /locus_tag="ACHK0Z_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU4502311.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(15915..17471)
                     /gene="gshA"
                     /locus_tag="ACHK0Z_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             complement(15915..17471)
                     /gene="gshA"
                     /locus_tag="ACHK0Z_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU4502312.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17544..17972)
                     /gene="yqaA"
                     /locus_tag="ACHK0Z_RS11495"
                     /old_locus_tag="pgaptmp_002299"
     CDS             complement(17544..17972)
                     /gene="yqaA"
                     /locus_tag="ACHK0Z_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU4502313.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17969..18535)
                     /gene="yqaB"
                     /locus_tag="ACHK0Z_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     CDS             complement(17969..18535)
                     /gene="yqaB"
                     /locus_tag="ACHK0Z_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU4502314.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18816..18892)
                     /locus_tag="ACHK0Z_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     tRNA            complement(18816..18892)
                     /locus_tag="ACHK0Z_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18856..18858),aa:Arg,seq:acg)
     gene            complement(19091..19167)
                     /locus_tag="ACHK0Z_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     tRNA            complement(19091..19167)
                     /locus_tag="ACHK0Z_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19131..19133),aa:Arg,seq:acg)
     gene            complement(19231..19307)
                     /locus_tag="ACHK0Z_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     tRNA            complement(19231..19307)
                     /locus_tag="ACHK0Z_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19271..19273),aa:Arg,seq:acg)
     gene            complement(19505..19581)
                     /locus_tag="ACHK0Z_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     tRNA            complement(19505..19581)
                     /locus_tag="ACHK0Z_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19545..19547),aa:Arg,seq:acg)
     gene            complement(19585..19677)
                     /locus_tag="ACHK0Z_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     tRNA            complement(19585..19677)
                     /locus_tag="ACHK0Z_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19641..19643),aa:Ser,seq:gct)
     gene            complement(19993..20178)
                     /gene="csrA"
                     /locus_tag="ACHK0Z_RS11530"
                     /old_locus_tag="pgaptmp_002306"
     CDS             complement(19993..20178)
                     /gene="csrA"
                     /locus_tag="ACHK0Z_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU4502315.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20413..23043)
                     /gene="alaS"
                     /locus_tag="ACHK0Z_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             complement(20413..23043)
                     /gene="alaS"
                     /locus_tag="ACHK0Z_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU4502316.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23171..23671)
                     /gene="recX"
                     /locus_tag="ACHK0Z_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             complement(23171..23671)
                     /gene="recX"
                     /locus_tag="ACHK0Z_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU4502317.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23914..24975)
                     /gene="recA"
                     /locus_tag="ACHK0Z_RS11545"
                     /old_locus_tag="pgaptmp_002309"
     CDS             complement(23914..24975)
                     /gene="recA"
                     /locus_tag="ACHK0Z_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU4502318.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25055..25552)
                     /gene="pncC"
                     /locus_tag="ACHK0Z_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             complement(25055..25552)
                     /gene="pncC"
                     /locus_tag="ACHK0Z_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU4502319.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25697..26782)
                     /gene="mltB"
                     /locus_tag="ACHK0Z_RS11555"
                     /old_locus_tag="pgaptmp_002311"
     CDS             complement(25697..26782)
                     /gene="mltB"
                     /locus_tag="ACHK0Z_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU4502320.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNRSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         6307 a         7143 c         6741 g         6697 t
ORIGIN      
        1 acgctatgcg aaaatgcaga tggcaatgag atccactgct ttcatctcca ttaacatccc
       61 attacgcttt tattaaggag cattagcatg ttctcaccgc agtcacgctt gcgtcatgca
      121 gttgcagata cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt
      181 ttcctctccg gaatgagctt cgaacagtct ttttattcca gattggtagc gattccggtg
      241 aacatcttaa ttgcatggcc atacggtatg taccgcgatc tgtttatgcg cgcggcacgc
      301 aaagttagcc cgtcgggctg gataaaaaat ctggctgata tcctggctta tgtgacgttc
      361 cagtcaccgg tgtatgtggc gatcttgtta gtggtgggcg cagactggca tcagattatg
      421 gcggcggtca gttcaaacat cgttgtttcg atgttgatgg gggcggttta tggctacttc
      481 ctcgattatt gccgccgact gtttaaagtc agccgttacc agcaggtaaa agcctgactc
      541 ttcttttcgc gactggcatc gccagtcgcg tctttaactg gcttcgccaa ataaaccatt
      601 caaataacgt tcaagcgcaa cacgcgaact aaagccatgc gggataccgt aatgctcgtg
      661 tcgctcgcct gctaaataga gcggaaattg ctggtaatga tcgcaagttt taatatcgga
      721 agcgggttca gccagtgttg aactgcgttc ttttaatgtt ggatgaatta ccagagcggt
      781 acgtcccatt cgcgcttcgc gattaacata gacataattt tccccacggc ggtaaccata
      841 cgctttctgg gttaccacat cgacagtaaa accgactttt tcaagtacgc gcgccacctc
      901 gtctggtcgt aaatacatat ttgatcctcg ttattatcaa ccgcgggcct accttacctg
      961 attgcgcatt cacaacgctt tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga
     1021 aaattatggt ctacactgaa aattacatcg aattctaatg ggggatcata tgtttaaccg
     1081 accgaaccgc aacgacgttg atgatggcgt gcaggatatc cagaatgatg tcaatcaatt
     1141 agctgacagc ctcgaatctg tattgaagtc ctggggcagc gacgccaaag gggaagctga
     1201 agccgcacgc agtaaagctc aggcattgct gaaagaaacc agggcgcgaa tgcatggtcg
     1261 tactcgcgtc cagcaagccg cacgcgatgc cgttggctgc gcggattctt ttgttcgtga
     1321 aagaccctgg tgtagcgtgg gtacagcagc tgcggtaggt atttttatcg gcgcactgtt
     1381 aagcatgcgc aaatcgtagt gcaaaaatga taataaatac gcgtctttga ccccgaagcc
     1441 tggcttcggg gtttcttttt gcctggtgaa tcacaaaaat ccccctaccc cgtcacgctc
     1501 atatccaggg taatttcgac cactatttgc tatatattgt gtggttgaat cttttttcaa
     1561 ctacatctag tatctctgta tcaacagaga tacaacccga cgcgtatcat cgcgccgtat
     1621 cttcatttta aacggaaata cgaatcatgc gcattactat ttacactcgt aacgattgcg
     1681 ttcagtgcca cgccaccaaa cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta
     1741 atgtcgatcg cgttcctgaa gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc
     1801 cggtagtgat tgctggcgat cttagctggt ctggtttccg tccggacatg attaaccgtc
     1861 tgcatccagc gccacacgcg gccagtgcat gagccagctc gtctacttct ccagcaactc
     1921 cgaaaacacg cagcgtttta tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa
     1981 tgagcgggaa cggattcagg tagacgagcc ttacatcctg atcgtgccct cttacggcgg
     2041 cggcggtacg gctggcgcgg tgccacgaca ggtaattcgc tttttaaacg acgagcacaa
     2101 ccgggcgttg cttcgcggcg ttattgcttc tggtaatcgc aactttggtg aggcgtatgg
     2161 ccgcgccgga gatgtgattg cccggaaatg cggcgtgccg tggctgtacc gttttgaact
     2221 catgggtacg caaagcgata tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg
     2281 acaaccgcag aatgcctgac gcaggaaacg atggattacc acgcgctgaa tgcgatgctt
     2341 aacctctacg atagcgcagg tcgcattcag ttcgataaag accgccaggc cgttgacgcc
     2401 tttattgcga cgcatgtgcg tccgaacagt gtgaccttca gtagccagca gcagcgcctg
     2461 aactggctgg tcaacgaagg ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac
     2521 tttgtcatta cgctgtttgc ccacgcacac accagcggtt ttcgtttcca gacattcctc
     2581 ggggcatgga agttttacac cagctatacg ttgaagacat tcgacggtaa acgttatctg
     2641 gaagattttg ccgatcgagt cacgatggtg gcgctgacgc tggcacaagg cgatgcgacg
     2701 ctggcgttgc aactgaccga tgaaatgctg tcaggacgct ttcagccagc cacgccaaca
     2761 ttcctcaact gcggtaagca gcagcgcggc gaactggttt cctgttttct gctgcgtatt
     2821 gaagacaata tggagtcgat tggccgggcg gtaaattccg cactgcaact gtcgaaacgc
     2881 ggcggcggcg tggcattttt gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt
     2941 attgaaaatc aatcttctgg cgtgattccg gtgatgaaaa tgctggaaga cgcattttcc
     3001 tatgccaacc agctcggcgc tcgtcagggg gctggtgcag tctatttaca tgcccatcat
     3061 cccgatattc tgcgttttct cgatacgaaa cgggaaaatg ccgacgagaa aatccgcatt
     3121 aaaacactgt cgctgggcgt ggtgatcccg gatatcactt tccatctggc aaaagagaat
     3181 gcgcagatgg cgctgttttc gccttatgac gtagagcgag tttatggcaa gccgtttgcc
     3241 gatgtggcca tcagccaaca ctatgacgaa ctggttgccg atgaacgcat tcgcaaaaaa
     3301 tacctcaacg cccgtgattt cttccagcga ctggcagaaa tccagtttga gtccggctat
     3361 ccctacatca tgtatgaaga cacggtaaac cgtgctaacc ctatcgccgg gcgcataaat
     3421 atgagtaatc tctgctcaga aattttgcag gttaacagcg cctcagagta tgacgagaat
     3481 ctcgactatg cccgtacagg ccatgatatt tcctgcaatt taggttcgtt gaatattgcc
     3541 cacaccatgg attcccccaa ctttgcccgc acggtagaga ctgccgtacg cggtttaacg
     3601 gcggtgtcag atatgagtca tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc
     3661 tcacacgcca tcgggctggg gcagatgaat ttacacggtt atctggcgcg agaaggcatc
     3721 gcttatggtt cgccggaagc actggatttc accaatctct atttctatac catcacctgg
     3781 cacgcactgc gtacctcgat gttgctggca cgcgaacgcg gtgaaacctt cgccgggttc
     3841 aaacagtcac gttatgccag tggtgaatat ttcagccaat atctgcaagg taactggcag
     3901 ccgaaaacgg cgaaagttgg cgaactgttt gcccgcagcg gtattacgtt acctacccgt
     3961 gagatgtggg cgcaactgcg cgacgacgtg atgcgctacg gcatttataa ccagaatctt
     4021 caggcggtgc cgccaaccgg ttctatctct tatatcaacc atgctacgtc gagtattcat
     4081 ccgattgtgg cgaaagtaga gatacgcaaa gagggcaaaa caggacgcgt ttactaccct
     4141 gccccgttta tgactaacga gaatctggcg ctgtatcagg atgcttacga aatcggcgca
     4201 gaaaagatca tcgacaccta cgcggaagcg actcgccatg tcgatcaggg tttgtcgctg
     4261 acgctgtttt tccccgatac cgccaccact cgcgatatca acaaagcgca gatttacgcc
     4321 tggcgcaaag gtatcaaaac gctctattac attcgcctgc gtcagatggc gctggaaggc
     4381 actgaaattg aaggctgcgt ctcctgtgca ctttaaggaa tatctatgaa actctcacgt
     4441 atcagcgcca tcaactggaa caagatatct gacgataaag atctggaggt gtggaatcgc
     4501 ctgaccagca atttctggct accagaaaag gtgccgctgt cgaacgatat tcctgcctgg
     4561 cagacattaa ctgtcgtaga acaacaactg acgatgcgcg tttttactgg cctgacgctg
     4621 ctcgacacgc tgcaaaatgt tatcggcgcg ccttcgctga tgcccgatgc actcacgcct
     4681 catgaagaag cggtattatc gaatatcagc tttatggaag cggttcatgc ccgctcttac
     4741 agttcgattt tctcgacgct gtgtcagacc aaagatgtcg atgccgccta cgcctggagt
     4801 gaagaaaacg caccgttgca gcgaaaagct cagattattc agcaacatta tcgcggtgat
     4861 gatccgctga aaaagaaaat cgccagtgtg tttcttgaat cttttttgtt ctattccggt
     4921 ttctggctgc cgatgtattt ttccagccgc ggaaagctga ccaataccgc agatctgatc
     4981 cgtctgatta tccgcgatga agcagtccac ggttactaca taggctataa atatcagaaa
     5041 aacatggaaa agatatctct gggacaacgt gaagagttga agagtttcgc cttcgattta
     5101 ttgctggaac tctacgacaa cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg
     5161 tgggctgacg atgtgaaagc gtttctctgt tacaacgcca ataaggcttt gatgaatctg
     5221 ggctacgaac cgttatttcc cgcagaaatg gcggaagtga atccggcaat cctcgccgcg
     5281 ctttcgccga atgccgatga aaatcacgat ttcttttccg gttcaggctc ctcttatgtg
     5341 atggggaaag cggttgaaac agaagatgaa gactggaatt tctgagggtg ttattttcaa
     5401 aaatatcact acccgcagca gggaaataat tcccgccaaa tagcttttta tcacgcaaat
     5461 attctgtagt gatctacact gatactctgt tgcattattc gcctgaaact acaatattca
     5521 ggcgtttttt cgctatctat gacaaaaaat atcaactttt ctcgatttgc tctcagccct
     5581 tatatcacgg gaaattccgg cgatttgctc gcatcaatat tcatgccaca tttgccctca
     5641 ggggttgcct cagattctca gtatgttagg gtagaaaaaa gtgactattt ccattgggta
     5701 atatatcgac ataaacaaat aaaggaatct ttctattgca tggcaattaa attagaaatt
     5761 aaaaatcttt ataaaatatt tggtgagcat ccacagcgag cgttcaaata tatcgaacaa
     5821 ggactttcga aagaacaaat tctggaaaaa actgggctat cgcttggcgt aaaagacgcc
     5881 agtctggcca ttgaagaagg cgagatattt gtcatcatgg gattatccgg ctcgggtaaa
     5941 tccacaatgg tacgccttct caatcgcctg attgaaccca cccgcgggca agtgctgatt
     6001 gatggtgtgg atattgccaa aatatccgat gccgaactcc gtgaggtgcg cagaaaaaag
     6061 attgcgatgg tcttccagtc ctttgcctta atgccgcata tgaccgtgct ggacaatact
     6121 gcgttcggta tggaattggc cggaattaat gccgaagaac gccgggaaaa agcccttgat
     6181 gcactgcgtc aggtcgggct ggaaaattat gcccacagct acccggatga actctctggc
     6241 gggatgcgtc aacgtgtggg attagcccgc gcgttagcga ttaatccgga tatattatta
     6301 atggacgaag ccttctcggc gctcgatcca ttaattcgca ccgagatgca ggatgagctg
     6361 gtaaaattac aggcgaaaca tcagcgcacc attgtcttta tttcccacga tctcgatgaa
     6421 gctatgcgta ttggcgaccg aattgccatt atgcaaaatg gtgaagtggt acaggtcggc
     6481 acaccggatg aaattctcaa taatccggcg aatgattatg tccgtacctt cttccgtggc
     6541 gttgatatta gtcaggtatt cagtgcgaaa gatattgccc gccggacacc gaatggctta
     6601 attcgtaaaa cccctggctt cggcccacgt tcggcactga aattattgca ggatgaagat
     6661 cgcgaatatg gctacgttat cgaacgcggt aataagtttg tcggcgcagt ctccatcgat
     6721 tcgcttaaaa ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg
     6781 ttagcagtcg atgcacaaac gcctcttagc gagttgctct ctcatgtcgg acaggcaccc
     6841 tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg
     6901 ctgctgcgcg ctttagatcg tgagggggta aataatggct gatcaaaata atccgtggga
     6961 taccacgcca gcggcggaca gtgccgcgca atccgcagac gcctggggta caccggcgac
     7021 tgcaccgact gacggcggtg gtgctgactg gctgaccagt acgcctgcgc caaacgtcga
     7081 gcattttaat attctcgatc cgttccataa aacgctgatc ccgctcgaca gttgggtcac
     7141 tgaagggatc gactgggttg ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc
     7201 ggttgattat atcctcaacg gtttccagca gttgctgctg ggtatgcccg caccggtggc
     7261 gattatcgtt ttcgctctca tcgcctggca gatttccggg gtcggaatgg gcgtggcgac
     7321 gctggtttcg ctgattgcca tcggcgcaat cggtgcctgg tcgcaggcaa tggttactct
     7381 ggcgctggta ttaaccgccc tgctgttctg tatcgtcatc ggtttgccgt tggggatctg
     7441 gctggcgaga agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac
     7501 cacgccagcg tttgtttatc tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc
     7561 gggcgtggtg gtgacgatca tctttgctct gccgccgatt atccgtctga ccattctggg
     7621 gattaaccag gttccggcgg atctgattga agcctcgcgc tcattcggtg ccagcccgcg
     7681 ccagatgctg ttcaaagttc agttaccgct ggcgatgccg accattatgg cgggcgttaa
     7741 ccagacgctg atgctggccc tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg
     7801 gctgggtcag atggtacttc gcggtatcgg tcgtctggat atggggcttg ccaccgttgg
     7861 cggcgtcggg attgtgatcc tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg
     7921 cgactcacgc agtcgcggca accgtcgctg gtacaccact ggccctgttg gtctgctgac
     7981 ccgcccattc attaagtaac tctgcacttg cccggtgacg ccgggcatta tcaccctgcc
     8041 aaaaaaagga ataacaatgc gacatagcgt actttttgcg acagcgtttg ccacgcttat
     8101 ctctacacaa acttttgctg ccgatctgcc gggcaaaggt attactgtta atccagttca
     8161 aagcaccatc accgaagaaa ccttccagac gctgctggtc agtcgtgcgc tggagaaatt
     8221 aggttatacc gtcaacaaac ccagcgaagt ggattacaac gttggctaca cctcgcttgc
     8281 ttccggcgat gcaaccttca ccgccgtgaa ctggacgcca ctgcatgaca acatgtacga
     8341 agctgccggt ggcgataaga aattttatcg tgaaggggta tttgttaacg gcgcggcaca
     8401 gggttacctg atcgataaga aaaccgccga ccagtacaaa atcaccaaca tcgcacaact
     8461 gaaagatccg aagatcgcca aactgttcga taccaacggc gacggaaaag cggatttaac
     8521 cggttgtaac cctggctggg gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga
     8581 actgacccat accgtgacgc ataatcaggg gaactacgcg gcgatgatgg ccgacaccat
     8641 cagtcgctac aaagagggca aaccggtgtt ttactacacc tggacgccgt actgggtgag
     8701 taacgaactg aagccgggca aagatgtcgt ctggttgcag gtgccgttct ccgcactgcc
     8761 gggcgataaa aacgccgata ccaaactgcc gaatggtgcg aattatggct tcccggtcag
     8821 caccatgcat atcgttgcca acaaagcctg ggccgagaaa aacccggcag cagcgaaact
     8881 gtttgccatt atgcagttgc cagtggcaga tattaacgcc cagaacgcca ttatgcatga
     8941 cggcaaagcc tcagaaggcg atattcaggg ccacgttgat ggctggatca aagcccacca
     9001 gcagcagttc gatggctggg tgaatgaggc gctggcagcg cagaagtaat ttttattcag
     9061 gcggataagg cgtttttgcc tcatccgccg ctctgtacac aatgcctgat gcgacgctga
     9121 cgcgtcttat catgcctaca aaatacactc attccgtatg gcggataagg cgttttcgcc
     9181 gcatccgccg ttctgtgcac aatgcctgat gcgacgctgg cgcgtcttat catgcctaca
     9241 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
     9301 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
     9361 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tcgccagtaa
     9421 ctattacgct cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
     9481 ggcaggcttt attgttaccg ccgcgcaatt gggctatgca gcaggtctgc tgtttcttgt
     9541 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcggc
     9601 aggcggcatg ttaatcactg ccagcagtca gtcgctggcg atgatgatcc tcggtacggc
     9661 attaaccggt ttattctctg tcgtggcaca aattctggtt ccgctggcag cgacgctggc
     9721 ttcaccggac aaacgcggca aagtggttgg cactattatg agcggtctgc tgttggggat
     9781 cttgctggca cggacagttg ccggattgct ggcgaatctc ggcggctggc gcaccgtctt
     9841 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
     9901 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagtatgtt
     9961 tatcagcgat aaaattctgc gtacccgcgc gttgctgggc tgcctgacct ttgccaattt
    10021 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    10081 tggtgtaatt ggtctgtttg gacttgcggg agctgccgga gcgttgggcg ctcgtccggc
    10141 gggcggtttt gccgataagg gcaaatcgca ccacaccaca actttcggtc tgctgctgct
    10201 attactttca tggctggcga tctggtttgg tcacacttcc gtactggcgt tgattatcgg
    10261 aatcctggtg ctggatctca ccgtgcaggg cgtgcatatc actaaccaga cggtaattta
    10321 tcggatacat cctgatgcgc gcaatcgcct gaccgcaggt tacatgacta gctactttat
    10381 tggcggtgcc gccggttcgc taatttcagc ctcagcctgg caacatggcg gttgggctgg
    10441 cgtttgtctg gctggcgcga cgattgccct ggttaactta ctggtctggt ggcgaggttt
    10501 tcatcgtcag gaagccgcaa attaagcaat cagcaaccgt tttcgtggct ttacacttat
    10561 aagggtgtta agaagcccat cagtctgata aggttaagat attcattcag tctatttata
    10621 atattaacaa tcgttaagcg tacactctat ggaaagccct actccacagc ctgctcctgg
    10681 ttcggcgacc ttcatggaag gatgcaaaga cagtttaccg attgttatta gttatattcc
    10741 ggtggccttt gcgttcggtc tgaatgcgac ccgtctggga ttctctcctc tcgaaagcgt
    10801 ttttttctcc tgcatcattt atgcaggcgc gagccagttc gtcattaccg cgatgctggc
    10861 agccgggagt agtttgtgga ttgctgcact gaccgtcatg gcaatggatg ttcgccatgt
    10921 gttgtatggc ccgtcactgc gtagccgtat tattcagcgt ctgcaaaaat cgaaaaccgc
    10981 cctgtgggcg tttggcctga cggatgaggt ttttgccgcc gcaaccgcaa aactggtacg
    11041 caataatcgc cgctggagcg agaactggat gatcggcatt gccttcagtt catggtcatc
    11101 gtgggtattt ggtacggtaa taggggcatt ctccggcagc ggcttgctgc aaggttatcc
    11161 cgccgttgaa gctgcattag gttttatgct tccggcactc tttatgagtt tcctgctcgc
    11221 ctctttccag cgcaaacaat ctctttgcgt taccgcagcg ttagttggtg cccttgcagg
    11281 cgtaacgcta ttttctattc ccgtcgccat tctggcaggc attgtctgtg gctgcctcac
    11341 tgcgttaatc caggcattct ggcaaggagc gcccgatgag ctatgaggtt ctactgcttg
    11401 ggttactggt tggcgcggcg aattattgct tccgctattt gccgctgcgc ctgcgtgtgg
    11461 gtaatgcccg cccaaccaaa cgtggcgcgg taggtatttt gctcggcacc attggcatcg
    11521 cctcgatatg cgctctgctg gttgtctcta ccgcaccaga agtaatgcac gatacacgcc
    11581 gtttcgtgcc cacgctggtc ggcttcgcgg tactgggtgc cagtttctat aaaacacgca
    11641 gcattatcat cccaacactg cttagtgcgc tggcctatgg gctcgcctgg aaagtgatgg
    11701 cgattatata acccacaaga atcatttttc taaaacaata catttacttt atttgtcact
    11761 gtcgttacta tatcggctga aattaatgag gtcataccca aatggatagt tcgtttacgc
    11821 ccattgaaca aatgctaaaa tttcgcgcca gccgccacga agattttcct tatcaggaga
    11881 tccttctgac tcgtctttgc atgcacatgc aaagcaagct gctggagaac cgcaataaaa
    11941 tgctgaaggc tcaggggatt aacgagacgt tgtttatggc gttgattacg ctggagtctc
    12001 aggaaaacca cagtattcag ccttctgaat taagttgtgc tcttgggtca tcccgtacca
    12061 acgcgacgcg tattgccgat gaactggaaa aacgcggttg gatcgaacgt cgtgaaagcg
    12121 ataacgatcg ccgctgcctg catctgcaat taacggaaaa aggtcacgag tttttgcgcg
    12181 aggttttacc accgcagcat aactgcctgc atctactctg gtccgcgctc agcacaacag
    12241 aaaaagatca gctcgagcaa atcacccgca aattgctctc ccgtctcgac cagatggaac
    12301 aagacggtgt ggttctcgaa gcgatgagct aacgcgtcat ctcgctcaaa aatccagact
    12361 tataaaagaa aaaatgactg gccagcatcg caacatgctg gcctttttgg caagcaggtc
    12421 ggctcagccg atgagttaag aagatcgtgg agaacaatat gagcgcaaat gcggagactc
    12481 aaaccccgca gcaaccggta aagaagagcg gcaaacgtaa gcgtctgctc ctccttctca
    12541 ccttgctctt tataattatt gccgtagcga tagggattta ttggtttttg gtactgcgtc
    12601 atttcgaaga aaccgatgac gcatacgtgg cagggaatca aattcaaatt atgtctcagg
    12661 tgtctggcag cgtgacgaaa gtctgggccg ataacaccga ttttgtaaaa gaaggcgacg
    12721 tgctggtcac tctcgacccg acagatgctc gccaggcgtt tgaaaaagcc aaaactgcac
    12781 tggcttccag cgttcgccaa acccaccagc tgatgattaa cagcaaacag ttgcaggcga
    12841 atattgaggt gcagaaaatc gccctcgcaa aagcacaaag cgactacaac cgccgtgtgc
    12901 cgctgggcaa tgccaacctg attggtcgcg aagagctgca acacgcccgc gacgccgtca
    12961 ccagtgccca ggcgcaactg gacgtcgcta ttcaacaata caatgccaat caggcgatga
    13021 ttctggggac taaactggaa gatcagcctg ccgtgcaaca ggctgccacc gaagtacgta
    13081 acgcctggct ggcgctggag cgtacccgta ttgtcagtcc gatgaccggt tatgtctccc
    13141 gccgcgcggt acagcctggg gcgcaaatta gcccaacgac gccgctgatg gcggtcgttc
    13201 cagccaccaa tatgtgggtg gatgccaact ttaaagagac gcagattgcc aatatgcgta
    13261 tcggtcagcc ggtcactatc accacggata tttacggcga tgatgtgaaa tacaccggta
    13321 aagtggttgg tctggatatg ggcacaggta gcgcattctc actgcttccg gcgcaaaatg
    13381 cgaccggtaa ctggatcaaa gtcgttcagc gtctacctgt gcgtatcgaa ctggaccaga
    13441 aacagctgga gcaatacccg ctgcgcattg gtttgtccac gctggtgagc gtcaatacca
    13501 ctaaccgtga cggtcaggta ctggcaaata aagtacgttc aacaccggtc gcggtaagta
    13561 ccgcgcgtga aatcagccta gcacctgtca ataaactgat cgacgatatc gtaaaagcta
    13621 acgctggcta atccagaggt gcgtgtgatg caacagcaaa aaccgctgga aggcgcgcaa
    13681 ctggtcatta tgacgattgc gctgtcactg gcgacattca tgcaggtgct ggactccacc
    13741 attgctaacg tggcgatccc cactatcgcc gggaatctgg gctcatcgct cagccaggga
    13801 acgtgggtaa tcacttcttt cggggtggcg aatgccatct cgatcccgct taccggctgg
    13861 ctggcaaagc gcgtcgggga agtgaaactg ttcctttggt ccaccatcgc ctttgctatt
    13921 gcgtcgtggg cgtgtggtgt ctccagcagc ctgaatatgc tgatcttctt ccgcgtgatt
    13981 caggggattg tcgccgggcc gttgatcccg ctttcgcaaa gtctattgct gaataactac
    14041 ccgccagcca aacgctcgat cgcactggcg ttgtggtcga tgacggtgat tgtcgcgcca
    14101 atttgcggcc cgatcctcgg cggttatatc agcgataatt accactgggg ctggatattc
    14161 ttcatcaacg tgccgattgg cgtggcggtg gtgttgatga cactgcaaac tctgcgcgga
    14221 cgtgaaaccc gcaccgaacg gcggcggatt gatgccgtgg ggctggcact gctggttatt
    14281 ggtatcggca gcctgcagat tatgctcgac cgcggtaaag agctggactg gttttcatca
    14341 caggaaatta tcattcttac cgtggtggcg gtggtggcta tctgcttcct gattgtctgg
    14401 gagctgaccg acgataaccc gatagtcgat ctgtcgttgt ttaagtcgcg caacttcacc
    14461 atcggctgct tgtgtatcag cctcgcgtat atgctctact tcggcgctat tgttctgctg
    14521 ccgcagttgt tgcaggaggt ctacggttac acggcgacct gggcaggttt ggcctctgcg
    14581 ccggtaggga ttattccggt gatcctgtcg ccgattatcg gccgcttcgc gcataaactg
    14641 gatatgcggc ggctggtaac cttcagcttt attatgtatg ccgtctgctt ctactggcgt
    14701 gcctatacct ttgaaccagg tatggatttt ggcgcgtcgg cctggccgca gtttatccag
    14761 gggtttgcgg tggcctgctt ctttatgccg ctgaccacca ttacgctgtc tggtttgcca
    14821 ccggaacgac tggcggcggc atcgagcctc tccaacttta cgcgtacgct ggcggggtct
    14881 atcggcacgt ccatcaccac caccatgtgg accaaccgcg agtcgatgca ccacgcacag
    14941 ttaaccgagt cggtaaaccc gtttaaccca aacgcccagg cgatgtacag tcaactggaa
    15001 gggcttggga tgacgcaaca gcaggcctcc ggctggattg cccagcagat caccaaccag
    15061 gggctgatta tttccgccaa tgagatcttc tggatgtcag ccgggatatt cctcgtcctg
    15121 ctggggctgg tgtggtttgc taaaccgcca tttggcgcag gtggcggcgg aggcggtgcg
    15181 cactaagtac aactaagcca gttcatttga actggctttt ttcaattaat tgtgaagata
    15241 gtttactgac taaatgtgca gttcctgcaa cttctcttcc ggcagcgcca gttcttcgtt
    15301 gctgttgatg cgcacgtcac gttccagaat gctacgcgca atatcctgcg cttcctgcaa
    15361 cgagtgcatc tggtaagtgc cacactggta gacgttcagt tccgggatct gattctgatc
    15421 ctgcactttc agcacgtctt ccattgccgc tttccaggca tcagcaacac gctgctcatc
    15481 tggcgtacca atcagactca tataaaaacc ggtgcggcag cccattggcg agatatcgat
    15541 aatctctaca ccattaccgt taagatggtt acgcataaaa ccagcaaaca ggtgctccag
    15601 ggtatggatc cctctttctg gcatcacttc tttgttcggc acgcagaagc gcagatcgaa
    15661 cacggtgatt gcgtcgccat gcggggtgtt cattgttttc gccacccgaa ctgcaggcgc
    15721 ttccatccgg gtatgatcga ctgtgaagct atctaacaac ggcatttagc cacctccggt
    15781 aattttttta aaaattttct gaactctttc ttcccaggcg agtctgagta tatgaaagac
    15841 gcgcatttgt tatcatcatc cctgaattca gagatgaaat tttggccact cacgagtggc
    15901 ctttttcttt tctgtcaggc gtgtttttcc agccacaccg caaacggttc ggtatcagcg
    15961 gcttccattt cctgctgacg gcgttcagac gcctcgcgct cggctacaaa atcctcttcg
    16021 cgcagaattt ccagcggctc ttcacgcagc agattacggt aggcttctgc aaatgctttg
    16081 cctgttccgc caataccagt atcaatcata gaccttaaga tacgggcaga gaaagtcaga
    16141 tcgggattat cgaagcaggc aaccagttca tcacacactt tctgatacgc ttcgccgccg
    16201 ttaatactat ccagcgtttg cgcgacgcgt ttcagatcgc ggaacagatc tttacccacc
    16261 tgcggtaacg ggaactgtgc ggtttcgcag ccgataccca gcgtcagacc cggtttgcga
    16321 ccttcgagga tcacccggtt ccagttaacg cgtgtacagg caagttcgct actgctcatt
    16381 tccggtgcat cagccagcgc acaccagacc ataaacaggt cgaggaatcg cacctgctgt
    16441 tcatctacac caatcggcga gaacgggttg atgtccagcg aacgcacttc aatatattca
    16501 atgccgccac gtaacagcgc atcagaaggc gactcgccgc tgcgggtaac gcgttttgga
    16561 cgaatcggcg cgtacagttc gttttcaatc tgcaacacgt tgctgttgat ttgcagcctc
    16621 ttaccgtctt tctcaatacc aatcttcgcg tactcttccg atggcgtttt gattgcctgt
    16681 ttaaggcccg ctacgtactc gtaaagatcg ttgaaggtaa taccaagatt gctttgcgat
    16741 ttattggtat agccgagatc gctcaaacga agagaggtcg catacggcag gtaatacata
    16801 ccgcactcgg ttttctcaaa cggcagcgac gttggttttc cttgcaggaa agaagaacaa
    16861 atcgccggag atgcaccaaa cagataagga atgacccaac cgaaacgata gtaattgcgg
    16921 ataacgcgga aatagcccgc agaaattttc tctttggcat cagcgcccga gatatcaccg
    16981 cacttcgctt gccagaatgc cattggcaaa gagaaattgt agtgcacgcc ggaaatggtt
    17041 tgcatcagcg cgccgtagcg atttttcagc ccttcacgat acagcgtttt aaagcgtccg
    17101 gtgttagaag tgccgtactg tgccagttcg atgtcctgac cttctgcgat gtagcatggc
    17161 atacttaacg gccacatccg ctcatcgccc atattgcgcg ccgtataacg atgcagatcg
    17221 cgcataaagg tcagcatatg ttcaatatca ccatccactg gtgtaatgaa ttccagcaat
    17281 gcttccgcaa aatcggtagt aatccatttg tgcgtcagtg cggaacctaa tgcttcagga
    17341 tgacctgttg ttgccagtgt gccatcagca ttaacacgca aagtttcgcg ctccagccca
    17401 cgctgtatcc cctttaacgc ctgaggatgt ttttccagcc aggccagcgc ctgtgatacg
    17461 tccgggatca aattgacctc ccgcctgtca aaatcgtttt aattagcata actgtaatgg
    17521 tgaccatatg tgcaggccta caattagtgc caccacatca tgccctgaac ggtcgctgct
    17581 gcaactgcaa catagcgtaa cgctttacca aggcacaaaa aaaagattac cggtccccac
    17641 gagatgcgca tccatcccgc taacagacac agtaaatcgc caaccacggg catccagctt
    17701 aataatagcg tgactgcacc atagcgtttc agccagccgg tagctttctc ttgccagcgc
    17761 gatgttttac gcaatggaaa gaaacgccca aggataacgt tagttaaccc tccaaggcta
    17821 ttacccattg ttgctgttaa aactaaaacc cagggatgac tgatcccgga aagcaacatt
    17881 gccaccagca cgacttcgga gttgccgggt aatagtgtag cgctgagaaa actactggca
    17941 aacaacgaga aaagcgataa cgcttcactc acagcaagcg aacatccacg gcgtccatgc
    18001 ctgctgcacg ggccgcctga ataccgaaat cggcatcttc aaagaccaca cactgcgtcg
    18061 gttgcacgcc catacgctgc gcgcacaaca aaaatgtgtc tggcgcgggt ttatggtgtt
    18121 tgacgtgatc agcagcgacg acggcgtcaa aataacggcg taatcccagg tgcgccagca
    18181 atgcctcagc gatggcgctt tcactccccg ttcctacagc cattgggcga cgaccatgcc
    18241 aacttttcac cacttcaaca agaggaagcg gctcgacact atccagcagc atacttctta
    18301 ctgcttctgt tttttcacgc gctaacgcat gcgggtcgag atcggcctga ttcagctcaa
    18361 taattgcctg agcaatacgc caggtgggcg atccattaag cgcaatcatt gcctgaacat
    18421 cgtactgaag accgtagtgc cctaatactt cgcgccacgc tttacggtgc gtaggctccg
    18481 tatccaggat tgtgccatcc atatcaaaaa ttaaacctgc ataacgctcg tacatggtct
    18541 tctcgcaagt cgaaaaatat gacgctactt tagcgtaatt gcttgatttt gtcgctgatg
    18601 gcgaaggaga agaaaattca ggaaatggat aaagtagtta tttagaaatg agatattttt
    18661 gaaggaaatt tttgggagaa gatggtgcat ccgggaggat tactcactgg cgttcgccct
    18721 tcgggccgtt gctgacgcaa cgttatcctc cctggtgcct gcggttaata ccgctatctc
    18781 ttcgatacct tcattgctga aggttacgga gaatatggtg catccgggag gattcgaacc
    18841 tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat gcatcgggaa
    18901 aatttatttt actgctgata ttgatatcgc tactaaagcc atatcaagta agagatggtg
    18961 catccgggag gattactcgc cttgcggctc gcccttcggg ccgttgctaa agcaacgtta
    19021 tcatccctgg tgcctgcggt taattccgct acctcttcga taccttcatt gctgaagatt
    19081 tcggagaata tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag
    19141 tactctatcc agctgagcta cggatgcatc gggaaaattt attttactgc tgatattgat
    19201 accgctacta aagccatatc aagtaagaga tggtgcatcc gggaggattc gaacctccga
    19261 ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc gggaaactta
    19321 ctttactgca gattttttga taccgctact aaagccgtat caagtaagag atggtgcatc
    19381 cgggaggatt actcgccttg cggctcgccc ttcgggccgt tgctaaagca acgttatcct
    19441 ccctggtgcc tgcggttata ccgccacctc ttccatacct tcatcgctga aagtacgtag
    19501 aatatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct
    19561 atccagctga gctacggatg caaatggcgg tgaggcgggg attcgaaccc cggatgcagc
    19621 ttttgaccgc atactccctt agcaggggag cgccttcagc ctctcggcca cctcaccaca
    19681 cgcctcttac gagtgcttcg aagaacttgt ttatcgctca tcgtcgctgc gtggcgcaca
    19741 tattactttc tgagacttat aagtcaaaca atttttccta cacttttatc gtttgcacac
    19801 ttcacgttca attagtctgt aaaaacgtca aaaagagtgt tttatcaaca gaaaaatgga
    19861 ggtctgacag atagtagtaa tgcaaaaaaa tggagactta agttgaatga acgggagtaa
    19921 agcgaaaaga ctatagagtg aaggagaaat tttgagggtg cgtctcaccg ataaagatga
    19981 gacgcggaaa gattagtaac tggactgctg ggatttttca gcctggatac gctggtagat
    20041 ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg ccaatacgta cctggttgcc
    20101 ctttacccct aaaactgtca cggtgacctc atccccaatc atgagggtct caccaactcg
    20161 acgagtcaga atcagcattc tttgctcctt gaaagattaa aagagtcggg tctctctgta
    20221 tcccggcatt atccatcata taacgccaaa aagtaagcaa tgacaaacac attacatgta
    20281 agcagtcatg gcattacatt ctgttaaacc taagtttagc cgatatacac aacttcaacc
    20341 tgactttatc gttgtcgata gcgttaatgc gaatgccgtg aagcgagtcc acggcattgc
    20401 ctgacgctta tattattgca atttcgcgct gacccagcct ttcacactgg ctaacgctgc
    20461 aggtaaggcc gcagcatccg taccaccggc ttgcgccatg tcaggacgtc caccaccctt
    20521 gccgcccacc tgctgagcga ccataccaat cagttcccct gctttcacac gatctgtgac
    20581 gtccttagat acgcctgcaa tcagagaaac cttaccttcg gctaccgttg ccagcacgat
    20641 aattgtcgac cccagctgat tttttaaatc gtcaaccatg gtacgcaaca ttttcggctc
    20701 aacaccgcta agctcgctaa ccaacagctt aacaccatta acatcaattg ccttactgga
    20761 aagatttgcg ctctcctgtg cggcagcttg ttctttaagc tgttgtagtt ctttttctaa
    20821 ctgacgcgta cgttccagta ctgagcgcac tttatccgcc agattattgc tatcgccttt
    20881 cagcagatgc gcgacttcgc ttaagcgatc gctgtctgca tgaacggtgg tgatagcgcc
    20941 ttctccggtt accgcttcga tacgacgaac gcctgcagca gtacccgatt cagagatgat
    21001 gcggaacaga ccaatatcac cagtgcggct ggcgtgagta ccgccacaca gctcggtgga
    21061 gaaatcgccc atgctcagca cgcgtacacg ctcatcatac ttctcgccga acagcgccat
    21121 cgcacctttc gctttcgccg cttcgagatc catgatgttg gtttcgatcg gcaaattgcg
    21181 acgaatctgt gcgttcacca ggtcttcgac cgcacgaatc tcttccggtt tcatcgcttc
    21241 gttgtgtgag aagtcgaagc gcagcacctt gtcgttaacc agtgaacctt tctgcgatac
    21301 atgagtcccc agaacctggc gcagcgcagc gtgcatcagg tgcgttgcgg agtgattcag
    21361 acgaatacgg gcgcgacgag cctcatcaac atcagcctgc acagcgtcgc ccactttcag
    21421 agaacccgca gcaagtttac cgatgtgacc aatcgcctgg ccgtatttct gcgtatcttc
    21481 caccgcgaag gagaagttag cgcctttcag ttcgccttta tcaccaacct gaccgccgga
    21541 ttccgcatag aatggtgttt gatccagcac gaccacagct tcctggcctg cattgatggc
    21601 atcaaccgct ttaccatcaa caaacagcgc ggtcactttg ccgttcagtt ccagatggtc
    21661 atagccttta aattcagatg cactatcaac acggatcatt gcgttgtaat cggcaccaaa
    21721 gccgctggct tcgcgcgcgc gacgacgctg ctcttccatt gctgcttcaa aaccagcttc
    21781 gtcaactttg atgttgcgct cacgacaaac atcagccgtc aggtcaaccg ggaagccata
    21841 ggtgtcgtac agacggaaag ccgtttcacc atccagcgta tcaccagaaa gttttgccag
    21901 ctcttcatcc agcaacgcca gaccgcgctc cagagtacgg gcaaactgct cttcttcagt
    21961 cttcagcacc tgctcaacct gcgcctgctg gcgtttcagg tcttcacccg cagagcccat
    22021 aacgtcgatc agcggaccaa ccagtttgta gaagaaggtt tctttcgcgc cgagcatatt
    22081 gccgtgacgc actgcgcgac gaatgatacg acgcagtaca taaccacggt tttcattgga
    22141 cggcattacg ccatccgcga tcaggaacgc acaagaacga atgtggtcag cgattacgcg
    22201 cagcgattta ttgctcagat cggtcgcgcc agtgactttc gctaccgcct ggatcaacgt
    22261 gcggaacagg tcgatgtcat agttagagtt aacgtgttgc agcaccgcag caatacgctc
    22321 cagacccata ccggtatcta cagacggctt cggcagcggt tccatcgtgc catctgcctg
    22381 gcggttgaac tgcatgaaga cgatgttcca gatctcaatg tagcggtcgc cgtcttcttc
    22441 cggacttccc ggagggccac cccaaatgtg gtcgccgtga tcgtagaaga tttcggtgca
    22501 cgggccgcac ggaccagtgt cacccatctg ccagaagttg tcagatgcgt atggcgcacc
    22561 tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc ccgacttctt tttcccagat
    22621 ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac agacgctctt tcggcagggc
    22681 aaaccatttt tcgctggtca gcagttccca tgcaaactga atggcatcgt gtttgaaata
    22741 gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta tggtgacgcg cggtgtaacc
    22801 gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg cagcgttggg aagtggtagc
    22861 gcgggaataa ttacgcttgt cgagcccaag gaacacatcc ttgaactggt tcatcccggc
    22921 gttggtaaac aacaaagttg ggtcgttatg gggtaccagg gagctgctgg caactacctg
    22981 atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg atctcagcgg tgctcttgct
    23041 cataattatc ctgaaatcaa gctaacgaaa tatcgccacc agctccagcg tgtcttaacc
    23101 gccgggctgg taactgaaaa gtgggaataa gataagtttt cttgactggg aagtaaaata
    23161 ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg gatatcttcc atcagatagc
    23221 cacgatagag cagaaaacgc tggatcttaa ctttttctga aaagacagtt ggcaaaggtt
    23281 caccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac
    23341 gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg attcagttcc tgacgaatac
    23401 gtgcaggtcc ataacctttg cggctacggc tggcgataaa gcgcgcaaca aatcggctgt
    23461 catcgagata gccatgttca tggcaccagg caataacgcg ctcgtaatct tctgccgtag
    23521 catcaatctc ttctgggcca tttttgccca taatcggtgc cgcgagttta cgtcgcagtt
    23581 cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac aggcgagcat
    23641 atgccgggcg acgggacgtt gattctgtca tggcatatcc ttacaactta aaaaagcaaa
    23701 agggccgcag atgcgaccca ggttttgctg ataaacagat ttaatagtca atgagtgtgc
    23761 agaatgttcc cgcaggccca attcgttaga ggatcgaggt gagagtaatt tttcacgcca
    23821 gtgctggcct gttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgtg
    23881 cacacattat ctattatcat caataacgaa cgattaaaaa tcttcgttag tttctgctac
    23941 gccttcgctg tcatctacag agaaatccgg cgttgagttc gggttgctca gcagcaactc
    24001 acgtactttc ttctcgatct ctttcgcggt ttccgggtta tctttcagcc aggcagttgc
    24061 attcgcttta ccctgaccga tcttctcacc tttgtagctg taccacgcgc ctgctttctc
    24121 gatcagcttc tcttttacgc ccaggtcaac cagttcgccg tagaagttga taccttcgcc
    24181 gtagaggatc tggaattcag cctgtttaaa cggcgcagcg attttgttct tcaccacttt
    24241 cacgcgggtt tcgctaccca ccacgttttc gccctctttc accgcgccga tacgacggat
    24301 gtcgagacga acagaggcgt agaatttcag cgcgttacca ccggtagtgg tttccgggtt
    24361 accgaacatc acaccaattt tcatacggat ctggttgatg aagatcagca gcgtgttgga
    24421 ctgcttcagg ttacccgcca gcttacgcat cgcctggctc atcatacgtg ccgcaaggcc
    24481 catgtgagag tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg ccgccacgga
    24541 gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg gcgtcacaga tttccagtgc
    24601 ctgctcgccg gtgtccggct gggagcacag caggttatcg atatcgacgc ccagtttacg
    24661 tgcgtagatt gggtccagcg cgtgttcagc atcgataaac gcacaggttt taccttcacg
    24721 ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta ccggaagatt ccggtccgta
    24781 gatttcgacg atacggccca tcggcagacc acctgcccca agcgcgatat ccagtgaaag
    24841 cgaaccggta gagatggttt ccacatccat ggaacggtct tcacccaggc gcatgatgga
    24901 gcctttacca aattgtttct caatctggcc cagtgctgcc gccaacgctt tctgtttgtt
    24961 ttcgtcgata gccattttta ctcctgtcat gccgggtaat accggatagt caatatgttc
    25021 tgttgaagca attatactgt atgctcatac agtatcaagt gttttgtaga aattgttgcc
    25081 acaaggtctg caatgcatac gcagtagcct gacgacgcac cgcatcacgg tcgccgctga
    25141 agcattcccg ccgggtaatg ccttcaccgc gggcagtggc aaaagcaaac cagacggtgc
    25201 cgacaggctt ctcttcactg ccgccatccg gcccggcgat accactaata gacacggcat
    25261 aatcagcacg agccgctttc agtgcgccta tcgccatttc caccacgacg ggttcactca
    25321 ccgcgccatg ctgcgccagc gtctcttcgc gtacgccgat catctgcgct ttggcttcgt
    25381 tactgtaggt gacaaatccg cgttcaaacc aggcggagct accggcaata tcggtaatca
    25441 ctttcgctac ccaaccaccg gtacaagact cggcagttgt tacggttgcg ccacgggctt
    25501 tcagcgcctg cccaacctgt tcacttaact gcatcagttc actgtcagtc atcacaacct
    25561 ctgtcagtca aaaatttatg ccggacaaga tagcactttc gtcaggaaga tggtgttggc
    25621 gttgcaattt tacgaggagg attcagaaaa aagctgatta gccagaggga agctcacgcc
    25681 cccctcttgt aaatagctac tgtactcgcg ccagcgccac ggcttgtcct aactgccaga
    25741 ccgccattgc gtaatgggtg ctgcggttgt aacgggtgat ggtgtagaag ttcggcagac
    25801 cgtaccagta ctggtagccg gtgccaacat ccagacgcag caggctggct tgttgatggt
    25861 tgcccagcgg ctgctgtggc gttaaacctg cggcggcaag ctgcgaaatg ctgtacttag
    25921 ttttgaagcc atttggcaag cctggagcct gaccgtttgc cattaccgcg acctgatcgc
    25981 ctttcaccca gccgtgcgct ttgaaatagt tcgccacgct accgatcgca tcaaccggat
    26041 cccacaggtt gatatgcccg tcgccgctga aatctaccgc atattgtttg taagacgacg
    26101 gcataaactg tccgtagccc atcgccccgg caaaggaacc tttcagattg agcggatcgt
    26161 cctgctcgtc gcgcgccatc aacaggaagg tttccagctc gccagagaaa tactccgcgc
    26221 ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt ttccccatca
    26281 cgcgccccca gcgggtttca acgccgataa tcccgacgat aatttccggc ggtacgccat
    26341 acacctgcca cgcgcgattc aacgcatctt catactgatt ccagaaaacc acaccgttct
    26401 gcacgttgtc cggcgtaata aattttttgc gataacgcag ccatgcgccg ttcggacctg
    26461 atggcggttt caccgatgtg gttggtgcct ggttatccat cagccgcagt accgaatcca
    26521 gacgtttcgc ctgggagaga atttcctgca actgctgacg atcgaaaccg tgtttgttca
    26581 ccattttgtc gatgaactgc tgggcattcg ggttattagc gaaatcgccg cccatctgca
    26641 tcacattgtg ctgcggctca agcaggaagc cgccagacgg cgttccggtg gtcgtatcag
    26701 tctcagtagg ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg ggaagcaatg
    26761 ttacataacg acgcttgaac atgaggggtc catttaacag attcaaccag gggcaagtat
    26821 ggtaaagcat cacgccccgc acaaggaagc ggtagtcact gcccgatacg gactttacat
    26881 aactcaac
//
LOCUS       DAZFWP020000060        26853 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFWP020000060 DAZFWP020000000
VERSION     DAZFWP020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.060"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1559
                     /locus_tag="ACHK0Z_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             <1..1559
                     /locus_tag="ACHK0Z_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HGU4502321.1"
                     /translation="NALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFMTTRV
                     FRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNK
                     LLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLC
                     QRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAI
                     CEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEI
                     GELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQII
                     KTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEI
                     TETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGD
                     IFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSA
                     WDKSGKLVKE"
     gene            1567..2895
                     /locus_tag="ACHK0Z_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     CDS             1567..2895
                     /locus_tag="ACHK0Z_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4502322.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2942..4267)
                     /gene="rimO"
                     /locus_tag="ACHK0Z_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             complement(2942..4267)
                     /gene="rimO"
                     /locus_tag="ACHK0Z_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU4502323.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            4480..4863
                     /gene="bssR"
                     /locus_tag="ACHK0Z_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             4480..4863
                     /gene="bssR"
                     /locus_tag="ACHK0Z_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU4502324.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            4974..6089
                     /gene="yliI"
                     /locus_tag="ACHK0Z_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             4974..6089
                     /gene="yliI"
                     /locus_tag="ACHK0Z_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU4502325.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQVGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRSGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6086..6712)
                     /gene="gstB"
                     /locus_tag="ACHK0Z_RS11585"
                     /old_locus_tag="pgaptmp_002317"
     CDS             complement(6086..6712)
                     /gene="gstB"
                     /locus_tag="ACHK0Z_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU4502326.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            6959..8161
                     /gene="dacC"
                     /locus_tag="ACHK0Z_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             6959..8161
                     /gene="dacC"
                     /locus_tag="ACHK0Z_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4502327.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8208..8966)
                     /gene="deoR"
                     /locus_tag="ACHK0Z_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             complement(8208..8966)
                     /gene="deoR"
                     /locus_tag="ACHK0Z_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU4502328.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(9024..9620)
                     /gene="ybjG"
                     /locus_tag="ACHK0Z_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             complement(9024..9620)
                     /gene="ybjG"
                     /locus_tag="ACHK0Z_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4502329.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9905..11137
                     /gene="mdfA"
                     /locus_tag="ACHK0Z_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             9905..11137
                     /gene="mdfA"
                     /locus_tag="ACHK0Z_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU4502330.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11178..11462)
                     /gene="ybjH"
                     /locus_tag="ACHK0Z_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             complement(11178..11462)
                     /gene="ybjH"
                     /locus_tag="ACHK0Z_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU4502331.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(11548..12363)
                     /gene="ybjI"
                     /locus_tag="ACHK0Z_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             complement(11548..12363)
                     /gene="ybjI"
                     /locus_tag="ACHK0Z_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU4502332.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(12363..13571)
                     /locus_tag="ACHK0Z_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             complement(12363..13571)
                     /locus_tag="ACHK0Z_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502333.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13655..14191
                     /gene="rcdA"
                     /locus_tag="ACHK0Z_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             13655..14191
                     /gene="rcdA"
                     /locus_tag="ACHK0Z_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU4502334.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(14366..16051)
                     /locus_tag="ACHK0Z_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             complement(14366..16051)
                     /locus_tag="ACHK0Z_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU4502335.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16321..16698
                     /locus_tag="ACHK0Z_RS11635"
                     /old_locus_tag="pgaptmp_002327"
     CDS             16321..16698
                     /locus_tag="ACHK0Z_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU4502336.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(16728..16985)
                     /gene="grxA"
                     /locus_tag="ACHK0Z_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             complement(16728..16985)
                     /gene="grxA"
                     /locus_tag="ACHK0Z_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU4502337.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17145..17432
                     /locus_tag="ACHK0Z_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             17145..17432
                     /locus_tag="ACHK0Z_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjC family protein"
                     /protein_id="HGU4502338.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDTLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            17416..18138
                     /gene="nfsA"
                     /locus_tag="ACHK0Z_RS11650"
                     /old_locus_tag="pgaptmp_002330"
     CDS             17416..18138
                     /gene="nfsA"
                     /locus_tag="ACHK0Z_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU4502339.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRQ
                     TIIKESRPFILDYLHKQGWATR"
     gene            18199..19101
                     /gene="rimK"
                     /locus_tag="ACHK0Z_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             18199..19101
                     /gene="rimK"
                     /locus_tag="ACHK0Z_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU4502340.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19189..19665
                     /locus_tag="ACHK0Z_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             19189..19665
                     /locus_tag="ACHK0Z_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU4502341.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            20017..21129
                     /gene="potF"
                     /locus_tag="ACHK0Z_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             20017..21129
                     /gene="potF"
                     /locus_tag="ACHK0Z_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU4502342.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKKKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            21224..22357
                     /gene="potG"
                     /locus_tag="ACHK0Z_RS11670"
                     /old_locus_tag="pgaptmp_002334"
     CDS             21224..22357
                     /gene="potG"
                     /locus_tag="ACHK0Z_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU4502343.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            22367..23320
                     /gene="potH"
                     /locus_tag="ACHK0Z_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             22367..23320
                     /gene="potH"
                     /locus_tag="ACHK0Z_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU4502344.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            23317..24162
                     /gene="potI"
                     /locus_tag="ACHK0Z_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             23317..24162
                     /gene="potI"
                     /locus_tag="ACHK0Z_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU4502345.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            24222..24710
                     /locus_tag="ACHK0Z_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             24222..24710
                     /locus_tag="ACHK0Z_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU4502346.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            24751..25878
                     /gene="rlmC"
                     /locus_tag="ACHK0Z_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             24751..25878
                     /gene="rlmC"
                     /locus_tag="ACHK0Z_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU4502347.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(26053..26784)
                     /gene="artJ"
                     /locus_tag="ACHK0Z_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             complement(26053..26784)
                     /gene="artJ"
                     /locus_tag="ACHK0Z_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU4502348.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         6607 a         6463 c         6954 g         6829 t
ORIGIN      
        1 ataatgcatt gttcgaaatc tatttttatt atcctggcgg taatttattg aacgcatcag
       61 ataaactttt ttatctaccg tttgctttca ttattatcgt gttgctggtg gtatatttca
      121 tgaccactcg tgtgttccgt cggcaatttt ctgaaatgac cgagctggtt aatacgctgg
      181 cgtttttgcc cgactcaacg gatcaaatcg aggctctgaa aattcgcgaa ggcgatgcga
      241 aagagattat cagcatcaaa aattcgatcg cggaaatgaa agatgccgaa attgaacggt
      301 caaataaatt gctctcactg atctcttacg atcaggaaag cggttttatt aaaaatatgg
      361 cgattattga gtccaacaat aatcagtatc tggctgtggg gatcatcaaa ctgtgtggtc
      421 tggaagccgt ggaagcggtg tttggtgttg atgaacgcaa taaaatcgtc aggaaattgt
      481 gtcagcgaat tgccgagaaa tatgcgcaat gctgcgatat cgtgacattc aatgccgatc
      541 tctatttact tctgtgtcgg gaaaatgtac agacatttac ccgtaaaata gcgatggtaa
      601 acgattttga cagcagcttt ggctaccgca atctgcgcat ccataagtct gccatttgtg
      661 aacctttgca gggggaaaac gcctggagtt acgcagaaaa actgaaactg gcgatttcca
      721 gtatccgtga ccatatgttc tccgagttta ttttttgtga tgacgcgaaa ctcaacgaaa
      781 tagaagagaa tatctggatt gcgcgtaata ttcgccatgc aatggaaatt ggcgaactat
      841 tcctcgtcta tcaaccgatc gttgatatta acacccgcgc cattctgggc gcggaggcgt
      901 tgtgccgttg ggtgtctgcg gagcggggga tcatttcacc gctgaagttc attaccattg
      961 ctgaagatat cgggtttatc aatgagctgg gttatcagat tattaaaacg gcaatgggtg
     1021 aatttagaca ttttagtcag cgtgcgtcgc tgaaggatga tttcttactg catattaatg
     1081 tttcgccctg gcagttaaac gaaccgcact ttcatgagcg ttttaccacc atcatgaaag
     1141 aaaatggcct gaaggcgaac agcctctgtg ttgagatcac tgaaaccgtg atcgagcgaa
     1201 ttaatgaaca tttttatctc aatattgaac aactgcgtaa acaaggggta cggatatcga
     1261 ttgatgactt tggcaccggt ttgtcaaacc tgaaacgttt ttatgaaatt aatccagata
     1321 gcataaaagt ggactcgcaa tttaccggcg atattttcgg tactgcggga aaaattgtgc
     1381 gcattatttt cgatctggcg cgctataacc ggatcccggt gattgcggaa ggcgtagaga
     1441 gcgaagacgt tgcgcgcgaa ttaatcaaat taggatgtgt tcaggcccaa gggtatctgt
     1501 accagaaacc catgccattc tccgcctggg ataaaagtgg aaaattagta aaagagtagt
     1561 ttacgtatgt ccagaatcaa taagttcgta cttacagtca gtctgctgat ttttatcatg
     1621 atttcagcag ttgcctgcgg gatctacact caaatggtaa aggaacgggt gtatagcctg
     1681 aaacagtccg ttattgatac tgcttttgcg gtggcaaata ttgctgaata tcggcgtagc
     1741 gtggcaattg atcttatcaa tacgctaaat cccacggagg aacagctgtt ggttggctta
     1801 cgcacagctt acgctgactc ggtttcaccc tcttacttgt acgatgtcgg tccttatctg
     1861 atttccagtg acgaatgtat tcaggtaaag gagttcgaga aaaattattg tgcagatatt
     1921 atgcaggttg tgaagtatcg acatgtcaaa aatacagggt ttatctcttt tgacggtaaa
     1981 accttcgtct attacctcta tccggtaact cacaatcgta gtctgatatt tttgcttggt
     2041 ctggagcgtt tttctttact gtcaaaatcg ctggcgatgg acagcgagaa cctgatgttc
     2101 tctctgttta agaacggtaa accggtgacc ggtgatgaat ataatgctaa aaacgccatc
     2161 ttcaccgttt cggaagcgat ggagcacttc gcctatttgc cgaccggatt gtatgtattt
     2221 gcgtataaaa aagatgttta tttgcgggtt tgtacattga ttattttctt tgccgcattg
     2281 gtggcagtga tatcgggtgc cagttgcctc tatctggtac gcagagtgat taatcgtggt
     2341 attgtggaga aagaagccat cattaataac cattttgaac gcgtactgga tggcgggctt
     2401 ttcttttcgg ctgccgatgt caaaaaactc tacagtatgt ataactcggc gttcctggac
     2461 gacctgacca aagcaatggg cagaaaatcc tttgacgaag atttaaaagc gctgccggaa
     2521 aaaggcggtt atttgtgcct gtttgacgtc gataaattca aaaacattaa cgacaccttc
     2581 ggtcatttgc tgggcgatga agtgttgatg aaagtggtga aaatccttaa atcacagatc
     2641 ccggtagata aaggtaaagt ctaccgcttc ggcggtgacg aatttgcggt gatttatacg
     2701 ggtggaacgc tggaagagtt gctatcgatt ctaaaagaaa tcgttcattt ccaggtggga
     2761 agcattaatt taagtaccag tatcggtgta gcacattcaa atgaatgtcc taccgtcgaa
     2821 cgcttgaaaa tgctggcgga tgagcggctg tataagagta agaaaaacgg cagggcacag
     2881 attagctggc agtaatcatt attcgcaggc cggacaaatg attttgcccg gcctgaatta
     2941 attaaacccg gctaccccac aaatcgtact catcggcgtg ctcgactttc acacgcagga
     3001 tatcacccgg cttaacgttg gtttcaccat tgagataaac cgcgccgtcg atttccggtg
     3061 catctgccat gctgcgacca attgcgcctt cttcgtccac ttcgtcgata atcaccagaa
     3121 tttcacggcc cactttctct tgcaggcgct cggcggaaat ctgctgctgc agctgcatga
     3181 aacggttcca gcgttcttct ttcacttctt ccggaacctg gtcaggcagg gcattggcgt
     3241 ctgcaccttc aaccgggctg tatttaaagc agccaacgcg atccagacgc gcttctttca
     3301 ggaagtcgag tagcatctgg aaatcttctt ctgtctcgcc agggaagccg acaataaagg
     3361 ttgagcgtag ggtcagttcc gggcagattt cgcgccactg tttgatgcgc gccagttggc
     3421 gatctacaga acccggacgc ttcatcagtt tgagaatgcg cgggctggcg tgctgcaacg
     3481 gaatgtccag atacggcagg attttgcctt ctgccatcag tgggatgacg tcgtccacat
     3541 gcggataagg gtaaacgtag tgcagacgtg tccagatccc cagtttcgat aactgttcgc
     3601 acaggctgac catgctggtt tttaccggct cgccgttgtg gaagccagta cgatgtttaa
     3661 catcaacgcc ataggcggaa gtatcctgcg agatcaccag aatctcttta acgcccgcat
     3721 ctaccagacg tttcgcttca cttaacactt cgccaatcgg acggctcacc aggtcgccgc
     3781 gcatagacgg aataatgcag aaggtgcagc ggtgattaca gccttctgaa attttcagat
     3841 aggcataatg acgcggtgtc agtttcacac cttgttctgg caccaggctc aggaatgggt
     3901 tgtgtttcgg ttttggcacg tagtgatgaa cgtgctccag aacctgctca tagctatgag
     3961 gcccggtgat ttccagcact ttcgggtgga cttcgcggat ctgatcttct tttgccccca
     4021 gacaaccggt cacaattacc ttgccgtttt cattcaacgc ttcaccaatg gcttccagtg
     4081 attcttgtac cgcgctgtca ataaagccgc aggtgttgac gatcaccatg tccgcatcgt
     4141 catagctcgg taccacgtca taaccttcag tgcgtagttc ggtgagaata cgctctgaat
     4201 cgacaaggtt tttcggacag ccaagggaaa caaagccgat tttcggctgg ggagttactt
     4261 tgctcatagc ttaaaaaata ttcagttaca ggaaaggtca gggcagggat tctacagagt
     4321 tctggataaa atttgtatcg caatctcatt cgctggcgga ggcgaaggaa atgtaaattt
     4381 tgttaatttg gcgtgaagaa ttgatcctgg acagcatttt gctcaaaaaa tagccatact
     4441 atttaattgc aacaaggctg gaaagaggag gatcgaagta tgttcgttga cagacagcga
     4501 atcgatctgc tgaaccggtt gatcgacgca cgcgttgacc tcgccgcata cgtgcaactg
     4561 aggaaggcaa aaggatacat gtccgtcagc gaaagcaatc atctacgaga taactttttt
     4621 aaactgaatc gcgaactgca cgataaatcg ctgcggttga atcttcatct ggatcaggaa
     4681 gagtggagtg ctcttcatca tgctgaagaa gcattagcga cagccgcagt atgtttgatg
     4741 agtgggcacc atgattgccc gactgttatt accgtcaacg ccgataagct tgaaaattgt
     4801 ctgatgagct taacgctgag tatccagagc ctgcagaagc acgccatgct tgagaaggcc
     4861 tgaaaactaa gggggagaaa gcgtctcccc cttcatgttt aagtttttgt aaaaatgaat
     4921 ttgttatctc ctccactgac tacgctttaa gccagagtca atccggaggc gttatgcatc
     4981 gacaatcctt tttccttgtg ccccttattt gtctttcttc cgctctctgg gcggctcctg
     5041 caacggtaaa tgtcgaagta ctgcaagaca aactcgacca tccctgggca ctggcctttt
     5101 tacccgataa tcacggtatg ttaatcactc tgcgcggcgg cgagttgcgt cactggcaag
     5161 taggaaaagg attatctgcg ccgctttccg gagttccgga cgtttgggcg cacgggcagg
     5221 gcggcctgct ggacgtggtt ttagcgcctg attttgctca gtctcgccgc atctggttaa
     5281 gttattccga agttggcgat gatggcaaag ccggaactgc tgtgggttat ggccgcttaa
     5341 gtgatgatct ctcaaaagtg accgacttcc gcaccgtctt ccgccagatg ccaaaactgt
     5401 ctaccggcaa ccattttggc gggcggctgg tattcgacgg taaaggttat ctttttattg
     5461 ctctgggcga aaacaatcag cgcccgacgg cgcaggatct ggataaatta cagggcaaac
     5521 tggtgcgtct gaccgaccag ggcgaaatcc cggatgataa tccttttata aaggaatccg
     5581 gtgcgcgcgc cgagatctgg tcttatggca ttcgtaatcc gcaaggaatg gcgatgaatc
     5641 cgtggagtaa tgcactgtgg ctgaatgaac atggcccgcg cggtggtgat gaaattaata
     5701 tcccgcaaaa aggcaaaaac tacggctggc cgctggcaac ctggggaatc aactattcag
     5761 gctttaagat accggaagcg aaaggggaga tcgtcgccgg gaccgagcaa cctgtttttt
     5821 actggaaaga ttcgcccgct gtgagcggca tggccttcta taacagcgat aaattccccc
     5881 agtggcagca aaaattattt ataggcgcgc tgaaagataa agatgtcatt gtgatgagcg
     5941 tcaacggcga caaagtgaca gaagatggcc gtattttaac ggacagaggg cagcgaattc
     6001 gtgatgttcg ctctggaccc gacggttatt tatacgttct caccgacgag tccagtgggg
     6061 aattacttaa agttagccca cgcaattagc taacgggaat catcaccact ttgcgcactg
     6121 cggggcgttc agtgagttgc tgataccagc gttgcagatt tggacgcggt gtccaggtca
     6181 ggccaacgtt aaacaaattg tagatgaacg gtgcgatagc gatatcgccc acaccaaact
     6241 cgtcgccaga gaaccatttt acttttgcca gttccgcatc gagcagggca aacagggcgt
     6301 cgcactcttt gcaactggca tcaatggcgg cctgatcgcg ctcttcgggt ggtgttctga
     6361 ctaatcccat caggatcccg cgatgagcat tactgagtgt ctggtttgcc cagtccatcc
     6421 atttttccgc ttccgcacga cgtgccggtg agtcgatcca caggcgtttt tgcccgtact
     6481 gtgccgccag atagcggaca atggcgtttg attcccaaag aatgagatca ctttcgtcgt
     6541 cacgcaacaa cggcaccagc ccgttagggt tcatcgccag aaaatcagca tcgtgattta
     6601 tcccaaactc acggcccgcg agaatttgct cataaggtag ttccagttct tcgagcgtca
     6661 gcaatacttt ttttacgtta gttgaattat tccgacccca cagcgtaatc atactcaccc
     6721 cttttccgac aggcagccac aggctgaaca ggactccgat ggtgaagtaa agttaacttt
     6781 taagcaacag ctggcaaaaa aatgacacca gaagcagcga agcggcagga tattgcataa
     6841 actttaaaaa ctttaccaac ttacggtttc tttaagtttg tgtgtgcgtt attaatcacc
     6901 gaacttatca tacggcgata taacgtattt tttttgaatg gatactcggg tggcatttat
     6961 gacgcaatac tcctctctcc ttcgtggtct tgcagcgggt tctgcatttt tattcctttt
     7021 tgccccaacg gcattcgcgg cggaacaaac cgttgaagcg ccgagcgtgg atgcgcgtgc
     7081 atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg cggatgagaa
     7141 actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc aggcgcttaa
     7201 ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct gggcgacggg
     7261 aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc aggtttcggt
     7321 ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta ttgcgctggc
     7381 cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt atgccaaaaa
     7441 actgggtctg accaacacta ccttccagac ggtgcacggt ctggatgcgc cggggcagtt
     7501 cagtaccgcg cgcgatatgg cattgctggg taaagcattg atccacgatg tgccggaaga
     7561 gtacgccatt cataaagaga aagagttcac cttcaacaaa attcgtcagc ctaaccgtaa
     7621 ccgtctgctg tggagcagca atctgaatgt tgatggcatg aagacaggaa ccacggcagg
     7681 cgcgggatat aatctggttg cttcggctac ccagggcgat atgcgtttaa tctccgtagt
     7741 gctgggggcg aaaactgacc gtatccgttt taatgagtct gagaaattat tgacctgggg
     7801 tttccgcttc tttgaaaccg tgacgccaat taaacctgat gccacctttg tgactcagcg
     7861 cgtctggttt ggtgataaga gcgaagtgaa tcttggggca ggtgaagcgg gctcagtgac
     7921 cataccgcgt gggcagctga aaaacctgaa ggcgagctac acgttaacgg aaccgcagct
     7981 taccgcaccg ctgaaaaaag gtcaggttgt cgggaccatt gatttccagc ttaacggtaa
     8041 atccattgag cagcgtccgc tgatcgtgat ggaaaatgtg gaagagggcg gattctttgg
     8101 tcggatgtgg gatttcgtga tgatgaaatt ccatcagtgg ttcggcagct ggttctctta
     8161 atcttctgat aaccggatgg cgcgaaacgt catccggtta tacgtcatta atacatcaac
     8221 ttaatacggt gcgtctgcgc gtacttcaca tactcatctt ccgggcaaca atcactcacc
     8281 acaatatcaa agcgtttcag gtcacccatg cgcgccggac gcaccttgcc aaatttactg
     8341 tggtcgacaa ccagcacatg cttttgcgcc atcgacatgg cccagtgttt taccggcaac
     8401 tcttcaagat taaaacaggt agcgccttta ctgacatgca cgcccgccgc ggaataaaaa
     8461 gcgatatccg ggcaaaaatt attcaacgtt tgctgaaaat cgatgggttt gaaaatggcg
     8521 ttgctggcgt gaaattcacc accgcacaga aatgcgcggc aatggggttt ctctttcagc
     8581 gccagaaagg tatttagcga ataacaaacg gcggtaaaag ggatttcatt atcaatcgct
     8641 tcaataatcc acggcgtggt ggtgccacag tcaaaaaaga gagtctgatc gggttctacc
     8701 agtgtcgcag ccatttttgc cgcccggcgt ttttcttcca ccaggcggga tttttgatcg
     8761 cttaacaggt aatggctggc actgcgcggt tccagaacaa tatagccgcc gagcaaaacg
     8821 acgggcgcac tgtggttgtt cagatcgcga cgaatcgtca tctccgaaac cccaagcagg
     8881 gcggcggcgt ctttaagatg taacttatcg ctgcgtttta attcttgcag cagctgcccg
     8941 atacgctctt cgcgacgtgt ttccataatc cctctgaata gttattgaag cgagccgctc
     9001 aatactacac tttttagcag agatcagtca cgcacccagc ctttgcggat cggtaatgca
     9061 aaacagaagc gataccacga ttgcagacgt tgatagagtt tatgccccat cgcttgccag
     9121 ataatctgag cgctaaggca gccaatcata cctgccagca atccaccgag catatccagc
     9181 ggccagtgca cgccaagata aacgcgcgac caggcaatga cgacggccag caccattaaa
     9241 agtgagccgg accacaggcg atgccagcat aaaaatgcca gtgcaaaggt gaaaatcacc
     9301 gtaccgtggt cgcttgggaa tgagtcatcc gccgcatgat gcaggaagtt atagccgata
     9361 ttttcgacaa agggtcggtc gtgcggaaaa agatgtccca tcgtccagga cacaaacagg
     9421 ctgaccgcca gcgcgatagc gatttttatc accagttgcc gttgtgctgt aagcccccac
     9481 aaccaaagca ccgcggccag cagcggtacc acggtaatca aatctttagc aataaaaatc
     9541 gccaacgaga tcatccacgg agccgagtct ggcgtcgcgt taataagaga gaatagagag
     9601 agattcaaat tttccagcat aacttcccga cgcaaagtga ttaaaaggga gccaatacag
     9661 gcaagtcgtt gagaataaag tgcaggttaa actgggtaaa gcggcatcgt cttatttccc
     9721 tcaagcggcc tgtttacggt gggtaattgt aacgggcata ggttaaataa aacttaaaga
     9781 aagcgtagct atactcgcaa taatgtaaga atgtgcttaa ccgtggtttc agctacaaaa
     9841 ttcgctttct cgttagctgt gcttttatta aactctgcgc gattattatt ggcgaagaaa
     9901 ttgcatgcaa aataaattag cttccggtgc caggcttgga cgtcaggcgt tacttttccc
     9961 tctctgtctg gtgctttacg aattttcaac ctatatcggc aacgatatga ttcaacccgg
    10021 tatgttggcc gtggtggaac aatatcaggc gggcattgat tgggttccta cttcaatgac
    10081 cgcatatctg gcgggcggga tgtttttaca atggctcctg gggccgctgt cggatcgtat
    10141 tggtcgccgt ccggtgatgc tggcgggggt ggtgtggttt atcatcacct gtctggcaat
    10201 attactggcg caaaacattg aacaattcac tctgttgcgc ttcttgcagg gcataagcct
    10261 ctgtttcatt ggcgctgtgg gatacgccgc aattcaggaa tccttcgaag aggcggtttg
    10321 tatcaagatc accgcgctga tggcgaacgt ggcgctgatt gctccgctac ttggtccgct
    10381 ggtgggcgcg gcgtggatcc atgtgctgcc ctgggaaggg atgtttgtct tgtttgccgc
    10441 attggcagcg atctcctttt tcggtctgca acaagccatg cctgaaaccg ccacgcgtat
    10501 aggcgagaaa ctgtcactga aagaactcgg tcgtgactat aagctggtgc tgaagaacgg
    10561 ccgctttgtg gcgggggcgc tggcgctggg attcgttagc ctgccattgc tggcgtggat
    10621 cgcccagtcg ccgattatca tcattaccgg cgagcagttg agcagctatg aatatggctt
    10681 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
    10741 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta ttattggtct
    10801 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
    10861 gttaagtatt tatgctttcg gcattggtct ggcgaatgcg ggactggtgc gattaaccct
    10921 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
    10981 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
    11041 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
    11101 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgctttgtgc
    11161 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
    11221 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
    11281 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
    11341 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
    11401 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataaac
    11461 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggt
    11521 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagcac
    11581 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
    11641 tgcgacgacc gcattgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
    11701 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
    11761 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
    11821 gccgttgccg gtgtggaccg gcaccataat atcgccgatg gcctcatgta atgctttttg
    11881 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaga tatcctctaa
    11941 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
    12001 ggcatcgtca tattttttga gcgtataggc gctatttttt ccgcaggcaa taatttccac
    12061 ttccgggcgc gtcagcaaat gttccacgac agtagtaaac gcatccttcg acagctcgcc
    12121 attaaaaaca tctttgcctt cgctcactac ccagccgccg ttttcagcca caaaggcaat
    12181 ttcattagca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
    12241 tacaaagcga attccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
    12301 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
    12361 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
    12421 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
    12481 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
    12541 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
    12601 agcgggaagc ccagcgaggc acccagtccc cagagtacaa cagacacccc agcgacccag
    12661 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagcgc actggcccga
    12721 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cagtgaagcg tccaacggtc
    12781 atccccaggg taaaaccggc ataaatcagc gagccggaag tggggctaaa accgtgaccg
    12841 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
    12901 accacaacac caatcagcag caactggata tcgcgataaa aaggtacgcc tttttcgcca
    12961 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
    13021 tatataggcg cgatgcctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
    13081 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
    13141 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
    13201 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
    13261 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgaccaa tgcgcaggac
    13321 atggtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
    13381 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
    13441 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
    13501 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
    13561 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
    13621 acatttgtac acaattgcag agagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
    13681 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
    13741 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
    13801 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga tcatgtcccg
    13861 gcaatatcag gcatttttta gcgatgttag tgatgctcca ggcgcatgcc aggctatcac
    13921 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
    13981 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
    14041 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
    14101 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
    14161 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
    14221 ccaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
    14281 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
    14341 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
    14401 tccctgccag cgtcagcagg acgttggcga ttgcataggt gcccgcatag cccagcgccg
    14461 ggatgttact gcgagctgta tcactgatga tctccattgc tggcgcgcag gtgcgtgcgc
    14521 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
    14581 aacagataac cacgggcaca agactgacaa tcaatccggc aatcaacatc tgaccgccaa
    14641 tcgcgcccag accgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
    14701 ccattaagcc gaactctttc accatgctta atgcaccctg aggaatgtaa ccgaaggtcg
    14761 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
    14821 ccatgccgaa actgaatgtg ctgaactgga atgtgatcat cccgatcatc agcccaataa
    14881 caaagaaggc gcagaatgcc agcaagtcag tgacctggct gtgaatcgag ataaagccga
    14941 tgcgatcggc gatggttttt acgcggcggg catcgccgct gacttgtaaa acgtcacctt
    15001 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
    15061 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttgccaacg gcgttatggt
    15121 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
    15181 ctttgccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
    15241 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
    15301 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
    15361 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
    15421 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
    15481 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
    15541 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
    15601 gcctgctttc catgccggaa tgacgcagtg tatcgccagc accgaccaga accggtgtcg
    15661 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
    15721 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
    15781 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
    15841 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
    15901 ctaataacag tgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
    15961 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
    16021 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
    16081 tgttgaaaga aatggtaatt tacgataatg ttttttacca gaattcaggg cgcagattca
    16141 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
    16201 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
    16261 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
    16321 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
    16381 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
    16441 ggcttgctat ttttcattct tcctggagca gtcgccagct tcttttcaca gcgtagagaa
    16501 gtcctgaaac ctctgtttgg cgcaatgctg gcggcaccct gttcaatgct tattatgcgg
    16561 ctgttttttt caccgacccg ctcattctgg caagagctgg catggttact aagcgcggtg
    16621 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
    16681 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
    16741 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
    16801 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
    16861 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
    16921 tcagccagat cttttgcacg cacacagtaa gggcaacccg gacgaccaaa aataacggtt
    16981 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
    17041 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
    17101 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
    17161 aattgcctaa aagcgtgttg atactggaat ttatcggaat gatgttgctg gcggtggcgc
    17221 tgctgtcggt aagcgacacc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
    17281 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
    17341 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
    17401 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggtcatcg ctccattcgc
    17461 catttcactg atgaacccat ttccgaagcg cagcgtgagg cgattattaa cagcgcccgt
    17521 gcgacgtcca gttccagttt tttgcagtgc agtagcatta ttcgcattac tgacaaagcg
    17581 ttacgtgaag agctggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
    17641 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
    17701 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
    17761 ttaaccgcag cggaatcgct gggattgggt ggggtatata tcggcggcct gcgcaataat
    17821 attgaagcgg tgacgaaact gcttaaatta ccgcagcatg ttctgccgct gtttgggctg
    17881 tgccttggct ggcctgcgga taatccggat cttaagccgc gtttaccggc ctccattttg
    17941 gtgcatgaaa acagctatca accgctggat aaaggcgcac tggcgcagta tgacgagcaa
    18001 ctggcggaat attacctcac ccgtggcagc aataatcgcc gggatacctg gagcgatcat
    18061 atccgccaaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    18121 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    18181 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    18241 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    18301 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    18361 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    18421 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    18481 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    18541 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    18601 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    18661 gagacgcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    18721 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggc
    18781 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gcgattttcg ttccaatttg
    18841 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    18901 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    18961 gggccgttgg tgatggaggt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    19021 ggtatcgaca tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    19081 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    19141 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    19201 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    19261 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    19321 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    19381 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    19441 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    19501 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    19561 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    19621 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    19681 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    19741 ttaaagatta tttcacttat cttgtgtcga tttggcttta tcacatagag caaatacgca
    19801 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    19861 tattcttgcg atgcttttat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    19921 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    19981 gttcgtttgt taacgaactt tcagaaggaa agagatatga ccgccttaaa taaaaaatgg
    20041 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    20101 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    20161 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    20221 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    20281 cttgaacgcc agctgacagc gggagttttc cagccgctgg acaaaagcaa attgccggag
    20341 tggaagaatc tcgatccgga gctgctgaag ttggtcgcca aacacgatcc cgacaataaa
    20401 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    20461 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa gcctgaaaat
    20521 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    20581 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    20641 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    20701 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    20761 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    20821 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaaa
    20881 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    20941 tccgacaatg tgttctatgc caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    21001 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    21061 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    21121 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    21181 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    21241 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    21301 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    21361 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    21421 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    21481 gccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccccatat
    21541 gactgtggag cagaacatcg cctttggcct gaaacaggac aaactaccga aagcggaaat
    21601 tgccacccgg gtcaacgaga tgcttgggct ggtgcacatg caggagttcg ccaaacgcaa
    21661 accgcatcag ctttccggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    21721 acgcccgaaa ctattactgc tcgatgagcc gatgggcgcg ctggataaaa agctgcgtga
    21781 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    21841 cacccacgat caggaagagg cgatgaccat ggcggggcgt atcgccatta tgaatcgcgg
    21901 gaaatttgtc cagattggcg agccggaaga gatctacgag catccaacta cccgctacag
    21961 cgctgaattt atcggttcgg ttaatgtctt tgaaggtgta ctcaaagagc gtcaggaaga
    22021 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgatg cggatgcttc
    22081 agtggtcgat aacgtgccgg tacacgtagc gctgcgcccg gaaaaaatca tgctttgcga
    22141 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    22201 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    22261 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    22321 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    22381 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    22441 gaagcatggg cgcaaactgg tcattgcatt gccatatatc tggttgatct tgctgtttct
    22501 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    22561 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    22621 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    22681 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    22741 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    22801 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    22861 taattttctg ctgtggctgg gggttataga ccagccgctg accattctgc ataccaatct
    22921 ggccgtttat atcggcattg tttacgctta cgtgccgttt atggtactgc cgatttatac
    22981 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    23041 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    23101 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    23161 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    23221 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    23281 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    23341 tcaccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    23401 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    23461 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggtttaagc
    23521 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    23581 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    23641 ccgctggtga tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    23701 gctcatgcca ttggctggcc tgcggaccgc ggtatgctca ccatctggct ggcgcatgtc
    23761 actttttgta cggcttatgt ggcggtcgtt atttcttcaa gattgcggga actggatcgc
    23821 tcgatagaag aagcagcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    23881 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    23941 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    24001 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct tgcaacgtta
    24061 attctcggtg cggtcggaat tgtcggattt atcgcctggt atctgatggc tcgcgcagaa
    24121 aaacagcgga tacgcgatat ccagcgtgca agacatggct gaagacacta aaatttgcca
    24181 acctggctac ataatgccgc gcatgccgcg gcattgtttt catggaagac gaaacgttgg
    24241 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    24301 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    24361 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    24421 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    24481 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    24541 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    24601 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    24661 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    24721 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    24781 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    24841 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    24901 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaacca
    24961 ctgctcggta tgctgcatcg cgacggcaca ccagaagacc tttgtgactg cccgctttat
    25021 cctgcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg tgcggggctg
    25081 acaccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    25141 cagagcgatg gcggcatgat gctgcgcttt gtactgcgtt ctgaaaccaa actggcacaa
    25201 ctgcgtaagg cgctgccgtg gttacaggaa caactaccgc aactgaaagt tattaccgtc
    25261 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta ccttactgaa
    25321 caacaggcgc tggcagagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    25381 ttccagacta atccggcggt cgccagccag ctgtacgcta ccgcgcgcga ctgggtacgg
    25441 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tgggtggctt tggtttacac
    25501 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg catcagaggc cattgcctgt
    25561 gcaaagcagt cagccgctga actgggctta acgcgtttgc aatttcaggc gctggactcc
    25621 actcagtttg ccaccgctca gggggaggtg ccggagctgg tgctggttaa tccgccgcgc
    25681 cgcggcattg gtaaaccgct atgtgattat ctctcaacaa tggcaccgcg ttttattatc
    25741 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    25801 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgctgacg
    25861 ctgctggtga agcaataaaa aagccgcagg tgcggcttca gattgctgat caggtgcgct
    25921 ttgttcatgc cggatgcggc gtgtacgcct tatccggcct acaaaatcat gctaaatcaa
    25981 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    26041 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    26101 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    26161 agcgatgccc agaccggtgc cgaagtattg cggatcggtc actttttcag tagcaacgcc
    26221 cagttgcgga ttggttttca gccattcgtt taccaccgct gtgtcaccaa ataccccatc
    26281 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    26341 cacttcgggg tgctgatcct gaatatattt ctggtgcgtg gtgccgtttt ccatcccaat
    26401 acgtttgcct ttcagatcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    26461 tgagttttca tagtagggcg tggtaaacga tacctgcttg ctacgctcgg gcgtgatatc
    26521 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    26581 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    26641 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    26701 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    26761 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    26821 ccgtgtctgc ggcatgggat aacaatgcca tca
//
LOCUS       DAZFWP020000061        26622 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFWP020000061 DAZFWP020000000
VERSION     DAZFWP020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.061"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(106..1107)
                     /locus_tag="ACHK0Z_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             complement(106..1107)
                     /locus_tag="ACHK0Z_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4502349.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(1113..1460)
                     /locus_tag="ACHK0Z_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             complement(1113..1460)
                     /locus_tag="ACHK0Z_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HGU4502350.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNSDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(1490..2140)
                     /locus_tag="ACHK0Z_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             complement(1490..2140)
                     /locus_tag="ACHK0Z_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502351.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(2156..2560)
                     /locus_tag="ACHK0Z_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             complement(2156..2560)
                     /locus_tag="ACHK0Z_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4502352.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(2651..2788)
                     /locus_tag="ACHK0Z_RS11720"
                     /old_locus_tag="pgaptmp_002344"
     CDS             complement(2651..2788)
                     /locus_tag="ACHK0Z_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502353.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            2860..3063
                     /locus_tag="ACHK0Z_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             2860..3063
                     /locus_tag="ACHK0Z_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4502354.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            3085..3435
                     /locus_tag="ACHK0Z_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             3085..3435
                     /locus_tag="ACHK0Z_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGU4502355.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            3446..3724
                     /locus_tag="ACHK0Z_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             3446..3724
                     /locus_tag="ACHK0Z_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502356.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHEIISEAEKARQVNGDKPYIEIICMDSEAEIQKAD"
     gene            3736..3978
                     /locus_tag="ACHK0Z_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             3736..3978
                     /locus_tag="ACHK0Z_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGU4502357.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGVHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            3975..4088
                     /locus_tag="ACHK0Z_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             3975..4088
                     /locus_tag="ACHK0Z_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502358.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIINSVKKIERFFSDE"
     gene            4182..4592
                     /locus_tag="ACHK0Z_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             4182..4592
                     /locus_tag="ACHK0Z_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502359.1"
                     /translation="MHAKEEGIIRALKEISKMESEVAKKAVANAHMDVATHTMIVAKV
                     TAEAAKIIEEQGVELALLKTKPVTGLDLSDTGRLIYTIGSEPQRYTIIAGLQNKYLIT
                     PHPIRESTLLTNLRLIERSQVAFIDDARHTVFNA"
     gene            4616..4819
                     /locus_tag="ACHK0Z_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             4616..4819
                     /locus_tag="ACHK0Z_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502360.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            4816..5082
                     /locus_tag="ACHK0Z_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             4816..5082
                     /locus_tag="ACHK0Z_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4502361.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            5079..5378
                     /locus_tag="ACHK0Z_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             5079..5378
                     /locus_tag="ACHK0Z_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502362.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            5701..5913
                     /locus_tag="ACHK0Z_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             5701..5913
                     /locus_tag="ACHK0Z_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGU4502363.1"
                     /translation="MANRKQQRAYAARRHIQTEINRRLFRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLRELQQLFQ"
     gene            6004..6393
                     /locus_tag="ACHK0Z_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             6004..6393
                     /locus_tag="ACHK0Z_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HGU4502364.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQEWQRVTGYQP"
     gene            6390..9230
                     /locus_tag="ACHK0Z_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             6390..9230
                     /locus_tag="ACHK0Z_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU4502365.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     AREEQEAKEEAFFAQSAMGKKLMASLEEAGQREKLAQSIISKRRATEQDPVARAFATL
                     KALPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWVRSLKRHERRALMRS
                     LRDKQAKNSSDEMQSWTQSRKQQRPLPDNHELLAREWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLA
                     QLGASDPEAATRLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKA
                     DLLRGGLTSGNKKQTETGFTNPVNEQKTRRDI"
     gene            9307..10266
                     /locus_tag="ACHK0Z_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             9307..10266
                     /locus_tag="ACHK0Z_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4502366.1"
                     /translation="MKICIDDGSTNIKLAWTENSERRNAISPNSFKSEWSAPFGGSQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            10271..10582
                     /locus_tag="ACHK0Z_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             10271..10582
                     /locus_tag="ACHK0Z_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4502367.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            10646..11016
                     /locus_tag="ACHK0Z_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /pseudo
     CDS             10646..11016
                     /locus_tag="ACHK0Z_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(11119..11649)
                     /locus_tag="ACHK0Z_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             complement(11119..11649)
                     /locus_tag="ACHK0Z_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502368.1"
                     /translation="MKKDSYPYLICMTVSVLGIIFLFYWWRADIYRITYLNSRISNYY
                     ILCSMGMSFFFSLFLVKRGVVKQSGWKGLSEYLKVYAGSCILTGIFLIIPLMMVTYFL
                     PGVTSSYVAPYEYTSGSSRSCSGAFVDDPELHKNILICYPYGNYEYDNIIYVEKKTNA
                     LGVVVTYAQTARDDAE"
     gene            complement(12180..13226)
                     /locus_tag="ACHK0Z_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     CDS             complement(12180..13226)
                     /locus_tag="ACHK0Z_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4502369.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(13226..14977)
                     /locus_tag="ACHK0Z_RS11810"
                     /old_locus_tag="pgaptmp_002362"
     CDS             complement(13226..14977)
                     /locus_tag="ACHK0Z_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGU4502370.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPKQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTRRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            15132..15968
                     /locus_tag="ACHK0Z_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     CDS             15132..15968
                     /locus_tag="ACHK0Z_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU4502371.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            15992..17044
                     /locus_tag="ACHK0Z_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             15992..17044
                     /locus_tag="ACHK0Z_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGU4502372.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            17090..17890
                     /gene="gpM"
                     /locus_tag="ACHK0Z_RS11825"
                     /old_locus_tag="pgaptmp_002365"
     CDS             17090..17890
                     /gene="gpM"
                     /locus_tag="ACHK0Z_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HGU4502373.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSTFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASTPQTQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            17992..18486
                     /locus_tag="ACHK0Z_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             17992..18486
                     /locus_tag="ACHK0Z_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU4502374.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAI
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILRRPRIF
                     AELC"
     gene            18486..18686
                     /locus_tag="ACHK0Z_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             18486..18686
                     /locus_tag="ACHK0Z_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU4502375.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            18689..19012
                     /locus_tag="ACHK0Z_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             18689..19012
                     /locus_tag="ACHK0Z_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4502376.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            19009..19401
                     /locus_tag="ACHK0Z_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             19009..19401
                     /locus_tag="ACHK0Z_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGU4502377.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGVR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPQYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            19398..19805
                     /locus_tag="ACHK0Z_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             19398..19805
                     /locus_tag="ACHK0Z_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HGU4502378.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASALKST
                     ADTLNIISAGVQDMQQVLAQLRAENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            19943..20410
                     /locus_tag="ACHK0Z_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             19943..20410
                     /locus_tag="ACHK0Z_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4502379.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            20403..21038
                     /locus_tag="ACHK0Z_RS11860"
                     /old_locus_tag="pgaptmp_002372"
     CDS             20403..21038
                     /locus_tag="ACHK0Z_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4502380.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            21050..21616
                     /locus_tag="ACHK0Z_RS11865"
                     /old_locus_tag="pgaptmp_002373"
     CDS             21050..21616
                     /locus_tag="ACHK0Z_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4502381.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVKLDTPVVECTNLLKTAEIDVTKGGKMSGNITHSGG
                     DFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            21613..21963
                     /locus_tag="ACHK0Z_RS11870"
                     /old_locus_tag="pgaptmp_002374"
     CDS             21613..21963
                     /locus_tag="ACHK0Z_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU4502382.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            21967..22863
                     /locus_tag="ACHK0Z_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             21967..22863
                     /locus_tag="ACHK0Z_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4502383.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLSEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            22856..23386
                     /locus_tag="ACHK0Z_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             22856..23386
                     /locus_tag="ACHK0Z_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU4502384.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            23389..24234
                     /locus_tag="ACHK0Z_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /pseudo
     CDS             23389..24234
                     /locus_tag="ACHK0Z_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            24754..25659
                     /locus_tag="ACHK0Z_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             24754..25659
                     /locus_tag="ACHK0Z_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4502385.1"
                     /translation="MASKGQVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHLKGMT
                     AVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQ
                     DGIKSHTHSASVSSTDLGTKTTSSFDYGTKTTSLFDYGTKTTSNAGSHTHSIPTSDGA
                     NQWGKIPLTSGRLATQYTSATTGSSGSHSHTVGIGSHQHTVGIGAHTHSVAIGSHGHT
                     ITVNAAGNAENTVKNIAFNYIVRLA"
     gene            25659..26237
                     /locus_tag="ACHK0Z_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             25659..26237
                     /locus_tag="ACHK0Z_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4502386.1"
                     /translation="MAFRMSKQSRTLKIYNLLAGTNEFIGDGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNTDEASWHLVEDHRGYTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTSWVKDAEAEKLFRSQEAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVDTTVAADIEWPVAPQ"
     gene            complement(26281..>26622)
                     /locus_tag="ACHK0Z_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             complement(26281..>26622)
                     /locus_tag="ACHK0Z_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGU4502387.1"
                     /translation="IINKFGCEIGLGANIGKGLTIPHHAGIVVHFAVDAGENLVLRQN
                     TTIGQIDGDIPGSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINKDIPSNCTY
                     ITKKSGVVLYK"
BASE COUNT         6987 a         7146 c         6726 g         5763 t
ORIGIN      
        1 actatcaacc accatttata aatcataaca ttatgatttt attaactttg aaaatggctc
       61 tatacaacgc catttgaatc tattgtcact ttttatcgcc actttttacc ctctattgtc
      121 aaaagggttc aattcaacag cagcctctaa atgacctgga gcaaaatgcg cataacgcat
      181 agtcattttt atatcgctat gccccagtat tttttgcaac acaagaatat ttccgccccg
      241 catcataaaa tgactggcaa acgtgtgacg tagcacatga gttaattgcc catcaggaag
      301 ctcgatcttc gctctcttaa ttgcagcgtc aaaagcctca taacatggtg aaaatagcgc
      361 tcctcgtttt ttgggaagca tagcctgcaa ttgaggtgaa atcggtacag tgcggttctt
      421 ctttccttta gttttaacaa atgtgattcg accgggcagt acttgagatt gctttaatcc
      481 ttctgcttca ctccaccgag caccagtcgc aagcccaata cggacaacaa cccccaaatc
      541 tttattccgt gactcatcac acgcaatcag aaggcgttca atctcatcta catacagaaa
      601 cgccagttcc ttttcttcct cacgaaactt gcgaatacca gtcagggggt tttcaccaga
      661 ccactcccca aggcgcttca gttcggcaaa aacagcatgt agatatgact gctcgcgatt
      721 aacggttgct tcactaagtt ttttcttccc cttgggattc cattctcctg atagccttct
      781 ttcccgataa gtggcaaaca tatttttgtc aaactgagaa gcaaatggat ctcccagcct
      841 ggaacaaatc gcctcaagtt tgactttgcg ctctgcacca gaggacaagg ttttaccgta
      901 catctcaaac caacgagcaa tcaactcaga aagacgagga ccagaaccat cttgaaactc
      961 gtctccaact ctactattca ttaaacggcg ctcataagag agcgcctcac tttttgtcgc
     1021 aaactgttta cgaatgcgtt ttcccgatgc cccgtaggga taacattcgc aaagccattt
     1081 acctgatgga atcttacgaa ccgacatttt agttacttat cacataaatc aaatgcagcc
     1141 ttagtgacat cccccagact cttttttaac cctggggcgg catcattatc tagccaaaat
     1201 ggattattgt tatctaacgg taacgcacca aatgttttac cttttattcg agccaaacct
     1261 gtaagtgcat ataacttatt atcgtcaaaa ttcatcacat aaggattacc atcaagacac
     1321 tgtaattgaa cctcatcaga attaaatggc cataccccat tgaaactctc acgttcaata
     1381 gttttaaaag gcattgcgac ggcggaaaaa gaaaacatag ataaaaaagt aactaatagt
     1441 tgaacctttt ttactttcat atcattacct caatttagct caagtaagat tacaaattaa
     1501 aaaacgccct agaaatgaca ccgcctacca aaacccctac gcagataaag aatattattt
     1561 cttttggata aagtcggatt aattctgaag cacgaagtcg gacttctggt aaggtcgaac
     1621 tctctgtgtg gcttgatgcc gattgttgct ctaaccacga caatgcagac tgtaactgag
     1681 aacgagtaag atcgtttaaa cgtcctgtac cgaaattgat atggcaatac cgcagaagtt
     1741 tttgtcgaag tccacagtct tcactgttac gtagtaataa acttacaaga gccttacagg
     1801 catcatgatc tttacatcgc tcaagcattg catgcagaaa actctccgct gttttatatt
     1861 gatttactgt catatcatca ataccagcta caccaatctc cgcatgtact ttttgccaaa
     1921 taataaacgc ttcagtattg ctagcttctg caatagcagc aaccaagcta tttagctcct
     1981 tacgctgagc cttaagcaaa gggcgatcgt catcatcatt attcgaaggg attgcgatat
     2041 tgacagtatg agaaccatca tatcgctcta tctgaatatt cttttcgtga aaatcacgcc
     2101 cagcaacgcg attgtttgaa ccgtttgagt tgacggccat gtcacctccc tactatcacc
     2161 tacccttagt ttcgttatag tcacgaccag cgatacggtt attaccacca gaaatattta
     2221 actcacgtcc tgatggctga gtttcctttt cactgatcgc accttttaaa gccccaatca
     2281 ccgcgttttt cacatctaac gaagctgctc gaaagcgagt aatcaactcc tgctcatcat
     2341 cgttataagt ttcaggtgag tgaattccca acacaacata ctgaacatca aggccaaaac
     2401 gagacagcgc tgccaaatac gcagcatcag gaaagctatc tcctttctca tatctaagct
     2461 gagttagctt tttgactcca ccaatgtcgc tcatggcaac ttgactaagt cccaatcttt
     2521 ccctttcctc acgcaaccgc tgaccaatat catttttcat acaaaaacct tgataggtat
     2581 ctttttttga taccaaaatg atttcacgag ctattagatg atcacaatat accactatga
     2641 aacaagttct tcacgatacc agatcacgca ttccgcgtaa caccgccaca ggtccaagac
     2701 tggcacttcg gctgtccctt gaggagcgag ccgtcattga tgaaatggca gctaaagaac
     2761 aacgctcatc ctctaacatg gcgcgcatga tcttccttcg cggcctagag ctaacccaga
     2821 aagaacaaaa caaatcttcc tgatcaggag gctagtggga tgtcaggtat aaccatcaat
     2881 atcaatgtga atgcccccta tgtatccctg cagaaatatg cagagataac aggtatccct
     2941 cttaatacgt gcaaaaagat gttggctgac ggtcgaatta ttatccgccc caaacgcgcc
     3001 aaaatggaaa agcctgaagt aaaccttgtg gcgatgttaa aagacgcttt ggctaacagc
     3061 taaaacaatg aacagagcac catcatgaaa aaaaacgcta ataatccata ctccaaattt
     3121 cgtaatggcg tagaacgcca tgtacaccac gtcgctacca gtgcatcacg tagtaacagt
     3181 cgctataacc tgaacgagac gcacgcaaca ccggatggcc acgctgtaaa acaaatcggc
     3241 gagcatgcct ggctgattga gaaagctgga atcgtggtcc acaaatgccc acgcaatccg
     3301 tttaccggaa accgcatttt tgcattgaac tgcggcgaca atcacttcgg gcaggatttc
     3361 acattatacg aagcacttcg cacggttgat cgtctgcttc gcgggcaaag ttttattaaa
     3421 caggctgatt tataacaggt gctttatgac caaagagcat gcacaaggtg tatttatccg
     3481 ttttattgat tttcgcggtg aactgttatt acgcgcatca gctattgatg gagtcgtccc
     3541 atcagaaaaa aatgcagcta cttacgttta tctgaacggc acgcgcctga ccgtagaact
     3601 tccgtaccag actgtacacg aaatcattag cgaagctgaa aaggcacgtc aggttaatgg
     3661 cgataaaccc tatatcgaaa ttatctgcat ggattcagaa gctgaaattc agaaagcaga
     3721 ttaaagggtg ttgcaatgga caaagaatat aaaacactca tcaacaaagc acttgagcgt
     3781 ttttattttc gcttaagcgc atcaggcgtt catgctgaac gtgcagcccg tgactcattg
     3841 accagggcaa tccggagtct gtatgacgtg gctttttacg ctgatgatct ggatgcactt
     3901 aacgaacttt ccgagctgat ctgtgccgca gaatgcggag aacatattga accgtataag
     3961 ctggggaata tcgcatgagt atatttatct catggcttgt tctgattatt tcggtggcct
     4021 gcgccattgg gattatgcga attattaatt cagtgaaaaa gatcgagcgt tttttctctg
     4081 atgaataacg atacaaataa aacatcaaat taaataagaa aacgtgaaaa ccatccgtat
     4141 taacggaggt attcgcacac gcaaataacg gagatacaaa aatgcacgca aaagaagaag
     4201 gtatcatcag agcactgaaa gaaatttcaa agatggaaag cgaagtagcg aaaaaagccg
     4261 tggccaatgc tcacatggac gtcgcaaccc acacaatgat agtcgcaaaa gtcacggcag
     4321 aggctgccaa aatcatcgaa gaacagggtg tggaacttgc gcttctcaaa actaaaccag
     4381 tcaccggact ggatttatct gacaccggac gccttattta cactattggc tcggagccac
     4441 agcgatacac cattatcgcc ggattacaga acaaatacct gatcactcct caccccataa
     4501 gggaatcaac gcttctgaca aatctccgcc tgatagagcg ctctcaagtt gcattcattg
     4561 atgacgcccg acacaccgta tttaacgcat agggttactg gacaaagggg gtgcaatggc
     4621 aattaagcat tttcccgtcg ttcgctttac ctccagaggg cgcgaatacg aggtcgacga
     4681 acgcctgatt accactatcg acaaacatcg ttcggaaaag gatgcacacc acatctacct
     4741 cactgacggt acttacttct gcgccaccaa cgtggcgcgg gtgaatctta tccgacaggt
     4801 acaggagcca cgcagatgac cattctggac tacatcgcta ctcatccggg ttgtagcggc
     4861 ggagagatcg ccgcagcact gaatactcca accacagcca ttaatgctga gttacgccaa
     4921 ctttggcgcg gcggcttagt catcagaaca aaccgcagca caggtggtcg cgctcgcaaa
     4981 actggaggcc aggcttctta ccacgtaaac ccgatgccgt tcgggtgtag caatccactt
     5041 actcacatgt ttaaccagct actaaaggaa gccagaacat gagcaccatc aaccaccaga
     5101 agctacgcga actggcattt gccctgcaac gaatggcaac gcctcaaaaa ttactggcat
     5161 ttcgcgcaat gctctcgccg tctgctgtgc tggcactgct ggatgagctg gagcacgcca
     5221 gaaccacggc tcctgccatt cgcctgacac tccatcatga aatcgctgat ttctgcgcga
     5281 cgttggaggc accaggcgaa ccggaaacgc cggaagcaat acagcaagag ctgctgcaac
     5341 gcattgacaa ggtttttgat ttttttctga accagtaaga aaccagaaca tgcacacaca
     5401 aaaaaaccgc ttgccatgcc gcaatcagtc aggttacatt tccgctgcac ctcataaaac
     5461 gggtgccggg attctcaacc cgatacagag caaagcgcat aaccgcgcca gcggtttttt
     5521 tgtgcgtact gtattgccac gtctttttcg cgtcagaatt atggcggggc gtacggggcc
     5581 gacttcggtc gggccggatt ctttgctctc cggtgttgag aaccctgtac gtctcgccac
     5641 cccgagattc tcaactctgg atggtgagct atttctatca ccgagcaaag aggccacacc
     5701 atggcaaacc gcaaacaaca gcgcgcatac gctgcgcgtc gtcacatcca gactgaaatc
     5761 aaccgtagac ttttccgcgc atcacgcgtc gcgcaaatca tgcacatcaa tatgctgcat
     5821 gagcgcagcc acgcgctatc gaacatctat tccgcctctg ttttcagcta tctggcggat
     5881 gatctgcgcg agctgcaaca actcttccag tagcaaaaca aactccatta attcctgttc
     5941 cgggcctttc ctgcaccttg cggcgggagg ccttcgcaca tctgtagtaa agagaattgc
     6001 agcatgattg acgctcatga cttcacaaga tgggtgcgca cacaggacac ccgtctggct
     6061 cccgttcttc agggattatt tgatctctac atccgtggtc gtgacaacag agcacgcacc
     6121 acaaaaccag agaatgcaga caccctttat ttcacagtag acgactgcta ccgcgtggac
     6181 ttcacaccac acgggctggc gttgcactgc ctgacaccac acggagaatc actgctggcg
     6241 tattacgact ccccggcctc cgtatttgcg gcaatgctgg cgcatcgcac tgctggcggg
     6301 tgtgcctcgc tgagtgaata caccgctgaa tttaaccgcc tttctgccct cttctcgcag
     6361 gagtggcagc gcgtgacagg ataccagcca tgagtgagtt tgcatggagc tggaatgaac
     6421 cacggccagc cattgatccg gccagattta cggagcacag gcaggaaact gaaaccgacc
     6481 tgcaacgcgc catccgttac taccttgagg cggacaaaaa ggcccgggaa gaacaggaag
     6541 cgaaggagga agcctttttc gcacaatccg ccatgggtaa aaaactcatg gcatcccttg
     6601 aggaagccgg acagcgtgaa aagctggcac agagcatcat cagtaagcgc cgggcaacag
     6661 aacaagaccc ggtggcccgt gcctttgcca cactgaaggc gcttcccgtt tatctgcgag
     6721 aacctctgag ccgccacctc tctttcctgc gcaagaagca ggaagccgat cgtcagaaag
     6781 gcaaaaagag ctggcaggcg gaacgctatg cacgcggaac cctgcgcaaa atattcgaac
     6841 gtctggaccg caccgacagc cgctggctga caccgggtta tcgctccctt gccggacgcg
     6901 aacgcctgga cgatttgctt tacctgccgc agctcaacaa acaccagata cagacgctgg
     6961 ccaccatgac ggcggcgatg ttcagcagca ccttcgaaaa actctgcgat ggctttggcg
     7021 cgactgatgg cgaactgacc atggatgtaa cgctgaaggc gtatcagatg ctggcccgca
     7081 tggcgttaca cctgcacgcc atgcctccac attatgacgc actgacaaca gacaaagacc
     7141 ggaggaacga accggacacg gagctgctgc cgggcgcaat ccttcgcctg acctgtgcgg
     7201 aatggtggaa acgcaaactg tggctgttac gttgcgagtg gagagaagaa caactccgcg
     7261 ccgcctgtct ggtttccaga aaaacatcac cctatctgag ccaggacgcg ttaagcgagt
     7321 ttcgcgcaca gcgcgagaaa acacgcgatt tcctgaaaag ttttatgctg gaaaacgaag
     7381 acgggttcac gattgatctc gaaacagtgt attacgcggg agtaagtaac ccggttcacc
     7441 gtaaggcaga aatgatggcc accatgaagg ggctggaact tctggccgaa gcccgtggcg
     7501 acaaagcggt gtttctgact gtcacctgcc cgtcaaaata ccacgccaca acagagaacg
     7561 gtcatccgaa tcccaaatgg aacggagcca ccatgcgcga ctccagcgat tacctggtta
     7621 acacgttttt tgcggcggtc cgcaagaaac tgaaccgcga cggcctgcgc tggtatggca
     7681 tccgcacggt ggagcctcac catgacggca ccgtgcactg gcatatgatg gtctttgctc
     7741 atccggaaga aatcgacacc attgtgtccc acacccgcga tattgccatt caggaagatc
     7801 gtcacgagct gggtgatgat attaccccac gctttaaggc ggagtacgtc gacggctcaa
     7861 aaggtacgcc aaccagctac atcgccacct acatcggaaa gaacctagac agccgcgccg
     7921 tggatggcat cgacccgaaa acgggcaagc cacgcgttga ccacgaaacc ggaaaatcaa
     7981 tggccgagag cgtggaacgc gccatcggct gggcgcgcct tcaccgtgtc cgccagttcc
     8041 agttctttgg tatcccctcc cgtcaggtgt ggcgtgaact ccgccgcctt gccagccaga
     8101 tggcacgcaa cccggaaggc ccgcaacggc tgaaggatga cgcaatggat gcggtactcg
     8161 ctgccgctga tgccggatgt tttgccacct acatagagaa acagggcggc gtacttgttc
     8221 cacgcaaaga ctacctgatt cgcaccgcct acgacctcgc agatgagctg aacgattacg
     8281 gcgaacagag tgtacagatt tacgggatct ggtcgccgct catcggggaa tcctcccgtg
     8341 tgtgcacgca tccggataac tggaagctgg taagacgtaa accggaagcg gaagacagcg
     8401 cccgcgaaaa tggttttgac cttcagggcg gccctgccgc cccttggact cgtggcaata
     8461 actgtccccg tgtacaggaa acggacaaca acgggacaga acagccggaa gaacggccag
     8521 caccgtggcc gcagcttcct gacggcgttg atgtggatga atgggtgcgc tcactgaaac
     8581 ggcacgaacg ccgggcgctg atgcgttcgc ttcgtgacaa acaggcaaaa aacagcagtg
     8641 atgaaatgca gagctggaca cagagccgca aacagcagcg gcctttgcct gataaccacg
     8701 aattactcgc tagagaatgg cgggagtctg ctgaatctct cggcctgcat atcggtgaac
     8761 aacaaatgca gcacctgtta cggggcggca gtctgtacgt tgacggcagc atcattgcac
     8821 cgcagggatt tgaaattgta cgcaaaccgg atacccgccc ggacagccga atcacgcagc
     8881 tctggcagcg cctgagccgt aatcacggtg taagcagcac ggagatccgc cataacccgg
     8941 tcgccagcta tctggcacag ctgggggcat cagaccctga agccgccaca cgcctggcat
     9001 ccacacttca gcaggaccag aacaccatga aaacacccgt taccgtgctt tctgacatgc
     9061 tgcgcgccat ccgcgacgca gagcacgcac agagaatcag tgaaaccact gaacgtgccc
     9121 gccgcaaagc agacctgctg cggggtggcc tgaccagtgg aaacaaaaaa cagacagaaa
     9181 cgggattcac aaatcccgta aatgagcaaa aaacgcgccg cgatatatga agcgcacaca
     9241 aaacaggcaa aaacgggatt tcagaatccc gtaaacgatt aattaatcaa cataaggaaa
     9301 agcgacatga aaatttgtat cgacgacggc tccaccaaca tcaagctggc atggactgag
     9361 aacagcgaac gccgcaacgc catcagcccg aacagcttca agtcggaatg gtctgcgccg
     9421 ttcggtggct cgcagcctgc gaactacatg cttgatggcg tgcgctatgg ttttgatccg
     9481 gtcagcgatc gctttgtcca gacgaccgac acgcaatacc agtacagcga tgtgaatgta
     9541 atcgccattc accacgcgct ggtcaaatca ggcatcacac cacaggaggt ggatgtggtt
     9601 gtcaccctgc cactgagcga gtatttcgac acaaacgcac agccggacat ggccaacatc
     9661 aaccgcaaaa aagcgaacgt tatgcgcccg gtggagtacc agaacggcga agcattcact
     9721 atccgtaacg tgcgggttat gcctgaatcc attccggctg gctttaaggc actggctgac
     9781 atgagtccgt ttgaatccct gctgattgtg gatttaggcg gaaccacgct ggatgtggca
     9841 aaggtccagg gacaactggc aggtatcagc caggtgtttt gcgatccaca cgtaggcgtt
     9901 tccctgatgg ccgatgccgt actgtcggtg atggccacta acggtatgcg taccagtcac
     9961 cacatcgcca ataccattat cgaacatcgc catgatgaag cctggttgcg ccaacacatc
    10021 cacaatgacg cgcattacgc cagcctgatg gcggttattc gtgaaaagga agaaacactg
    10081 aaacaacgcg tgatccgcgc gctggcgggt ttttcgggtt acggacgggt gatggttgtc
    10141 ggtggcgggg cggagattgt ggcacccgct atccgcgaag cctgcggagt taatgcgact
    10201 ttcatcgcgg acggggtgcc acagtttgct ctggttaatg ggctgtacgc aatggacaag
    10261 gagtaaacca atgacgacac caaccagacg gataagtttc tatctgaagc ccgccgccgt
    10321 caagaacgaa ggcgaagcat gcgcctggct ggacagcctt acaccagaag cccgcaaaag
    10381 cggccaacgt gtggcttttc tggccgggct ggcacttctg aaaatgaatc cggcagaggc
    10441 ttaccgactg gctgcatggg ctgatgatga agcgttatca gtgacacaaa ccaggacaga
    10501 acgccccgca tcacagccag tatcaaccgc acagataacc agtcagatgg ccggaaatat
    10561 ccgggcgtta tttcctgaat aacacaacat cagggcgagt tcgccctgct ctccacctca
    10621 gaacataaac aaggagaacg acttaatgag cgaaatcaac tatcaggcat tacgtgaacg
    10681 ttattcacct gtgccagtac cgaaatgccc tatttgcggc gaggaaatgt caattcagcg
    10741 aatatctgga gcacaggttg tttatgcctg ctccggttat ggtgatgatg gagatttcaa
    10801 aattggccga actcttgccg acgaacatta tgaaaaatca cacgtaacag tgttggatgt
    10861 cgggatcctg aagtattggc gctacttgat tggctggaaa ccaaagacaa ccgaatcgct
    10921 gaactggaaa aaatcgccac tgactatgca cttaaattcc agaaagcaca ggacgcatta
    10981 aagtacgccg ctttgctgca tagcaggaca gcgcagctaa aatactaact taaagcggaa
    11041 gattaataca tggtgagtcg gggtaatttg tttttatccc aacaaataaa caaaaaagtg
    11101 gactatctct gtagtatctt attcagcatc gtctcgtgca gtctgtgcat acgtcacaac
    11161 aacgcccaat gcattagttt tcttttcaac atagataata ttgtcatatt catagttacc
    11221 atacggatag caaataagaa tatttttatg cagttcggga tcatcaacaa acgctccgga
    11281 acagcttctt gaactaccag atgtatactc atacggtgca acatatgatg atgttactcc
    11341 tggtaaaaaa taagtcacca tcataagagg gataatcagg aatatccctg taagaatgca
    11401 agagccagca taaaccttca ggtattccga cagtcctttc cagccacttt gcttcactac
    11461 ccccctcttc accagaaaca aggagaagaa aaaggacata cccatactac acaagatgta
    11521 ataattggat atacgcgaat taagatatgt aatcctgtag atatctgccc tccaccagta
    11581 aaagaggaaa ataatcccta gcactgaaac agtcatgcaa atcaaataag gatatgaatc
    11641 tttcttcatg tcttgcgccc attaaaattt ccctgctacg aacaaattta ccatccgttt
    11701 tttgcgcaga aaacagcacg atgcacactg cacgatagtg cacaaatttg cacaattttt
    11761 ttgaacgact ttttgccctt ccggcccccg tggcggctgg atccgtcaag gatccgtgcg
    11821 tgcacaaaaa aacgcgtttt ttctgcgcgc aggtgacggg ggaacagccc gcgtttcagg
    11881 gggtaaatag cattccctga acgatgtcgc agagatacaa cagaatggct gtatttctca
    11941 cgctgagcgt gaaaaagacg tgagggcttt tgatttgatg gggtgaaagg taaggccgtc
    12001 aaaatcgcac tgagacggcg agaacatgca gtcaacgcgg tgggattgcg taagagtctg
    12061 actgtcgatg atggcaatca gcaggaaagc gtcgtgaaat tatctgactg atacaggagc
    12121 tggagagtcg gggcataaat tttttatgcc ccggcgaagc agcagacaag cgaagcgcgt
    12181 caggatgtgg gctgggtgtc taacagtgcg taagggttaa agcggatcac ctcttcccca
    12241 agccagtcat tgatgtgctt catggcctcc atgacgggca tcagctcgtt aattgcgtaa
    12301 acccgcgcgg ccttctccac atcaccaaac gcactttttt cgcccggcat cgcccccatc
    12361 agttgcggcg gaacgcggtg cgcagccagc acatcatcac gggatgccgc cttaacattc
    12421 atgaactcat cctttgcggt gatctgctgg aacggcaaaa tttgcacccc ctctttgccc
    12481 ccgttgggcg catggatgag cacgttttta aacgcgccac caccacgcgc accctgtaac
    12541 gtttctttca gggagtccat gctttcgcgg tttacctgcg ctgcaccgat gtagatgatg
    12601 cacccggcgt gggatccatt gtcgtaatac agttttctga acatgtccgc cgaatgagac
    12661 aggctggccg agagtaatgc gccaagatat tccggcatgc cgtaaatttc ctggttaata
    12721 tccggattca ttaggtggca cactttgcca gggcgaaact ggaacgcgtc cttgccatcc
    12781 tgcacatacc accatgattc aagatcgctt ccgcgtcgca tgtatttcgc cagtgcgtgc
    12841 cgtaatttaa gcggttcgcc gagcatattg cttctaagct caaggaatgc gttaccgaac
    12901 acaaaccagt ccagcgccag cgccgagaaa tcctgccggg aaagcagcgg gtgcgggatg
    12961 tagcaaccga gtaatacatt gcgcttaaag taaagcgcag actgatgcca ggacgtttgc
    13021 cgggctgctc ttgccagacc gtaccagtcc accggggttt cataccaccg cccgttatca
    13081 gcacagtaca tattgtccag caggtcatgc ccggtcaggc gataaggacc atcaaatgtg
    13141 aatgcactga gcgatgattc tttcctgagc gcatcagcga gatcaatgcg tgaactcatg
    13201 cgcacttttt tattttttct gctcatcaga actccataac cgtgaaacgc tcgttttctc
    13261 cttcgccgcc aatcggttcg ttaatgacag caagcatggt tgcccacgca aggtcgccgt
    13321 ggctgatccc cctcgcgcgg tccgtttcgt aagtgataaa gccgcccggt gttttcacct
    13381 tacgcacggc gttaaaggcc gcgaccagct cgcgttcggc gcgatcgtat tcccaccgtc
    13441 cggcacgcat tatttgcagc attttcagta ccagcgaccg tttggatgac agcgtgaagg
    13501 tgtacggaat agcagcaggg aaaaaccgct tcactatctg ataaacagcc tccccgttcc
    13561 cgcccgtcac atcaatgccg atgtgttcca cgttgtagcg acgcgtgaac tcttcaatga
    13621 ctctggcctg ttcttcaaac tccagcccct gaacgcgtcg cgtctccacc gttcgaaaac
    13681 ggccaccagg aacagccgga ggaaccacca cggacacagc gccgctgtcg ccgttgccac
    13741 tgctgccgtt tgcgtcatac ccaatccata ccggacgatt ccccatcggg cggggagcaa
    13801 aaggtttcca gtctttccag tcgtcgtatc cgtcaacacc gcagccaatc aggatattca
    13861 ggttaaatgc cgattcccct tcgcgaacaa actcacacat atagagattg aggaactcgt
    13921 cttcggtgtt ttcatcacga atttcatcaa tatcggtgtg tttccagccg tgattaacca
    13981 catcttccag cgtgacaatt tgccgccacg tccggtcagg gcagataagc ccgttatgca
    14041 gcgttttcca gtccacagaa aaacgctggc gtttatgcgt ggcctttttc tcgttccagc
    14101 ggtcgccgtt ccagtaggcg tatgcctcgt gcgtttcggt ggatggcgtg gagaagtagg
    14161 tgcgccgcag tccgctgagg gttgccatag cgccagccac cttgcgcagt tcagcaaagc
    14221 gactgaccca gaagaattca tcaaaataaa aattgcccgt ataggactgt gccgacgcag
    14281 cagaagtgcc gagaaaatgc agctctgcgc cgttggagag gatgatttta tcgccccctt
    14341 tcagctccac atcaacttca gccgcggcct tctgaataat gcttttaaac tggaacgcct
    14401 gacgacgcga cgcagacaaa aaaatctggt tacgctggta aggttgcgcc acatcgtcac
    14461 gcagcgccat cagcagagct tcctgtgcaa aataccaggt cgccccaatc tgtcgggatt
    14521 tcaggatcat cctgttacgt atccctgctt ccctgcaaag ggtcagggag tcaaaccagc
    14581 cccgctgatg ccactccagc ctgctgatga ttttttcccg cagtgcggca atctgtttcg
    14641 gcgtgaaatg atttttgagt tttttcgccc ggcctttctt tcctgcggcc atcacatccg
    14701 gctggccatc atgcagcttt ttaagctgcc gggtcagcag gtctatttcc ttaaagtcac
    14761 cgcctgtttt attctgtttt tcagtaagct ggatgaggcg cgcatcgatg gactgcgtga
    14821 cacgctgcac gggtggcgtt tcatcccact ggtcacgttt tttccacgca taaatcgtgt
    14881 tcgggtttat tcccatcaga cgtgatattt ctgcgggcgg ataaccctgc cagtaaagtt
    14941 gccgcgcacg ctggcgcaca aaagcgtcct gaatcattgc tccccctgag taattacagg
    15001 aagattaccc gcgcgcgaaa ctgttctcct taaccctctg ttctgactgt tttcttacaa
    15061 caaaagccct ttgtatcagc ctgttacgct ttgccatcat gactgaagaa ccagtcagag
    15121 gggcaaaaac tatggctaat gaaaaaaaga catcccgcaa aaagtttcgc gtggctgtct
    15181 ccggatcaac tgttgatggc cgtgaaatca gtccggtgca tctgcgtgaa gccgccgaga
    15241 acttcaaccc ggatgtttac gctgcccgcg tgaacgttga gcactatctc tcgccatgcc
    15301 cgtcaagcga attttccgca atgggcgatg tcaccgcact gagtacggaa gacattacgg
    15361 aaggtccgct ggccggacgt actgcgctgt atgcagaaat cgaaccgacc gagcgcatga
    15421 agcagcttgt cgcggacggc aagaaaatct attccagtat cgaactgcac ccgcagttct
    15481 ccgttaacgg gcgcgcctat ctggtcgggc tggcgatgac cgacaccccg gcaagcctgg
    15541 gcactgagcg cctgaaattc acggcacagc aacgtcaggc ggtgatgacg ttcaacagta
    15601 tccagggtga agcgccgctt atctccgaag ccatcgagtc tgaaatcatc gaaatggcag
    15661 aacaacgcca ggaagaaggc acccagtggt ttaaccgcgt aatggggatt attggccgtg
    15721 gccgcaaagc ggatgacgcc agtttttccc gtattcagga agcggtggaa ggcgtcgcaa
    15781 cgtcacaggc cgacattatc gaccgtttta atgtgctgga aacccgccat cagcaggacc
    15841 gccagaaaat tacgtcactg accacagagc tggcagcact gaaggaaaaa ctgcgcacgc
    15901 aggacggcga tccgcagaac cggttcaccg caacaggtgc agcctccgac cagctggctg
    15961 acttctgata agacaaagga gcaaattttt tatgaatctg gtgatgtcag atattacccg
    16021 caacaagctg ggttgctata tggcgcagca ggcgtcgctt aataatattc cggtttccgc
    16081 actggtatcg cgatttaccg tggaaccctc ggtgcagcag cgttttgaaa acgcctcaaa
    16141 ggaaagcacc gaatttacaa aaagaattaa cgtgatcggc gtgaccgacc agaaaggcga
    16201 aaaaatcctc ctggacacca ccgggccaat tgcacgcacg aataccagtt atgacggcac
    16261 aaaacgccgt aacccgaata acgtggttga tctgaaaaac cgcaaatacc agtgcgaaca
    16321 ggtgaactac gacacgttta tttcatatcc gcagcttgat gcctgggcgg cacatcctga
    16381 ttttcagtcc cgcatcagcg cacagattgc ccgacaggtg gcgcttgacc gcatcatgat
    16441 cggtttcaac ggcacgtctc acgcggatga gtccaacttc agcaccaaca agctgcttca
    16501 ggacgttaac gtgggatggc tggagcacat cagaaccgac gccagcgaac gcgttatgaa
    16561 tgacgtgacg ctgacctccc gcaacatgga caacaccgtg gcgcacgcgg gtaagtatgc
    16621 gaacgctgat gcactggtac aggacgcgcg ttcatccctg ctggatgaat ggcacaagga
    16681 agctgacgac cttgtggtga ttatggggcg caacctgttt aactcgctgc gtctgcccgt
    16741 gctgaacagc atcagcggcc agaatccaaa tgcggaatta cttgccgggc agctcatcct
    16801 gtcatcgcgc accattggcg ggctgggcgt gttccttgcg ccgttcttcc cggatgcaac
    16861 gatgctgatc acctcgttca acaacctgtc gatttactgg cagaaaggtt caatgcgtcg
    16921 tctgatgaaa gacgaaccgg aatacaaccg catcgccacc taccagtcca tcaatgacgc
    16981 ttatgtcgtt gaagactatg gcaagtgcgc gatggtcact ggcctgaagt tcgccgacag
    17041 ctaatcaact cacggcgggc atcatgcccg cctgtaacgg agagaaaaaa tgattactcc
    17101 tgcacagcaa cactggcaga acgtgatggc acagcgcgca ggccgggcga atgaaggcgt
    17161 ggaccacgcc gcgcgtaccg cgcatgaaga ggtgctgtat cgtctgcgtc tggcacaggc
    17221 ccggcttaag ggcgtacagg ccagaagcgc gaaagccgcc atcaaaaaag agttgttgcc
    17281 ggacttttcc ggctggattg agggaacgct ggaggctgac ggcgggcagc aggatgaagt
    17341 gattgccacg ctgatggtgt gggcgattga ctgcggcgat cttccgcttg cgctgcgtat
    17401 tggtgcgtat gtggtccgtc acaacctcat catgccggat aactttggcc gtactgctgc
    17461 cacggtactg accgaagaaa tctgtaaccc ggtattgacg caggccggga cggatgccga
    17521 cgcggatttg tccaccttta tcgaaccact ggacacactt tgggaaattg tcgccaacca
    17581 ggatatgccg gacgaagtgc gcgccaaatt atgcaaggcg tgcgcctttg cccgccgtgg
    17641 cctgagcgat gcggacagca tggcctcatc actgaagctg ctgcgcgaag cgatgcacct
    17701 gaacccgaac gcaggtgtga aacgcgagat tgcaaccctt tcccgcgccc tgaaaaaagc
    17761 tgattccgca gccgcaccag aagatgccag cacaccgcag acgcaggacg aaagcagcaa
    17821 aagtaaaaag acaacgcgga agcctgcaac acgaaaaacc accgcgacgc agaaggcgaa
    17881 gcgcggttaa cgactgaccc cgtcagcggg cggcgtgcgc ggtgttccgg tttgactccg
    17941 tgaccgttta caccgcgcac ccaccgcccg attttttcag gagtgaaccc catgagtatg
    18001 gttgccagaa ccaaccccgg acccgcagag gacgacatca ccgataccga tgatggtgat
    18061 acccgtattt cagcgggtgc attctggccg gatattgtgc tgcgtgagct gcgtctggcg
    18121 atacgactgc cgggccgcgt gaccacctcc cgcctgctgc ataccgccac cggggccgtg
    18181 gcacacgtta cccgcgagct ggaagcgtgg cagcaggaac agcaggcggc tggccatcag
    18241 acgctggccg atgttccggc ccccgtaatt aacggagaaa gcgtcaatct ctggcactgg
    18301 cgcaatgctg tttataccgc cacacgcgcc ctgattctgg agcgttaccg cgatgcggac
    18361 acaacggata agggcgaccg ccgggcggac gcactggata tacagacatc ggatttgtgg
    18421 cgcgatgtga gctgggccat ctctgacatt ctgcgccgcc cgcgaatctt tgcggagctg
    18481 tgctgatgaa agtgaaggca ctggaaggcg acaccgtgga ttcgctctgt ttccggtact
    18541 acggcacgac gcagggcgtc accgaaaagg tgctggatgc caaccccgga ctctgtcagc
    18601 aggtatttct ggacgccggg caggaagtgg agatgccgga gccggagaag aagaaacgag
    18661 aaatgattca gttgtggggg gagtagcagt gagcaccatt caaacaggga tcacagagca
    18721 ggttattgcg tggctctttg accacctgcc aacggtgtat gcagtaggcg cggcagtcag
    18781 catttccgcg ctgatgagtc tttatgacgg acgaacactg gttcagaccg taacgggatc
    18841 gctggcgtgc ggcgttcttg ccatggccgt ggccgggtcg ttgcgcttct tcgggtttcc
    18901 tgaagatgcc gtgacgttta tcggcgcatc aatcggtttt atgggcgcag agaaagcacg
    18961 cgacaaggtt attgcggcct ttaatcgcag ggtgaaggag aaggacgaat gagcaacaca
    19021 tttaaattca gcagccggag cgaaaagaat ttgcagggtg taaatcctga tctggtgaaa
    19081 gtgacccgac gggcactgga aatctcggaa gtggattttg gtatcaccga aggggtgcgc
    19141 agccgttacc gccagaagca acttgtggcc acgggtaaga gccagaccat gaacagccgc
    19201 caccttacgg ggcatgccgt ggatgttgtg gcttatgtcg gcagccaggt gtcatgggaa
    19261 tggccgcagt acgaaaaaat cgcagcagca ttcagacagg ccagccggga actgaatatt
    19321 ccggtggaat ggggcggaga ctggaagacc ctgaaagacg gaccgcattt tcagttacca
    19381 cacggagcct atccggcatg aagctctggc ccacgctggg tgtcgctttc cttctgattg
    19441 ccgcatgggg aacatccatg cgtctgtcgt ggtcgctggg tcgggagaac gccagaaacg
    19501 aagcgcaggc cagcgccctg aaaagtaccg ccgacaccct gaatatcatc agcgccgggg
    19561 tacaggatat gcagcaggtg ctggcacaac tccgcgcgga aaatcagcaa cgcaatcagg
    19621 acggagaggc cagacgtgaa cagctacgca acgatattgc aaaagatgaa tgcgcccacg
    19681 ctttgcctga cgctcgtttt actgacaggt tgcgcaggca cgcagaacgc gccacggcca
    19741 gcgccgtcag tccggcttat accgcagacg ctgaccatac cggtaatgcc tccccccttc
    19801 cctgatactc ccacatgggg aaatctcggt atatggggcg accgccttct ggatgcactg
    19861 gaaacctgta acgcggataa acgggccatt gaattactgg aacagcgccg gctgcaacga
    19921 ctgaacaacg aggacaacaa ccatgctgaa aactgattcc ctgcgtgaag ccatgacccg
    19981 ttcatgccga tggtgtcagg ccaacccgga aaaattcacc attttcgtgg agagcggcaa
    20041 cattgaaacg accggagaaa cgccctcgtt tgtttaccgc tatcagatgg tgatgtttgt
    20101 catggattac gccggggagc tggacgacct cacgctgccg ctactggcgt ggttatccga
    20161 aaatcagcca cagttgttgc tcaaccctga gcgtaatcag gacatcaaat tctccgccgt
    20221 tatcaatgac gatgacagcg ccgatctcct gtttacgctc cccctgcggg aacgcgttcg
    20281 cattacgcgc aacagtcagg ggacaccgca ggcagaacac ctgccggagc caaaaccccg
    20341 tctgccctct tccgaaggcg actggtcgca tgtattccag gatgtgacgt ggggtgaaag
    20401 cgatggataa ggcattcacc cgcgtggatg aaacctttga ggccatccgc gacagcctga
    20461 atcagcaggc catcaataac atcgccagaa agctggcaca ggatttacgc cgcgcccagc
    20521 aggcgcgcat ccggtcacag aaagcgccgg acgggaccgc atggacacca cgcagacgcc
    20581 gcgtaacccg gatacaggag cgcattcgct ttatctggaa taacgaagca cgcacgctga
    20641 aaaactggca tcacgacacg gggaaatacg ggcgaaccat taccgggtgg gatgaggata
    20701 aaaacaatat ccgcacgttt taccgggatg acatcgaccg ttttctggaa atacgcaccc
    20761 ggcgcatcaa ccaggacagc acaaagcgcg tccccatgtt cgtaaaactg cgcaccgccc
    20821 gctacctgaa agcccgtgca gatgcttccg gtgtgacggt gggttacagc ggcgtggctg
    20881 cacgtattgc acgcgttcat cagttcggtg agcgcgatca ggttgcgccg ggcattttca
    20941 ccgattaccc ggtacgtgag ctgctgggta tcagccaggc agatgaacgc ctgatttata
    21001 acacggtgct gggccggatt gcggaggctg tacggtgagc gcagaactca tgcgactgct
    21061 gagcaacatc atccgcaccg ggattatctc tgaagttgat gagaagtcct ggtgcgtgcg
    21121 cgttcgcagc ggcgaactgg aaacaggctg gttgcgctgg aacaccacgc gcgcgggagc
    21181 cttcaatgtg tggctgccgc catcacccgg cgaacaggtg gtaattgcct gcattggcgg
    21241 caacccggaa accgccatga taattggcag cctgtggagt gatgccagtc cggcacccgg
    21301 caaaagcctg aaagaaatcg tggtcagcgc gccggacggc gcggtgttcc gctacgacgc
    21361 ggacgcaggc gcactgagtg ccagcggcat gaaaacggcc actttgcagg catccgtcag
    21421 cgtgaaactg gatacgcccg tcgtggaatg cacaaacctt ctgaaaacag ccgagattga
    21481 cgtcacaaaa gggggaaaga tgagcggcaa tatcacgcac agtggcggcg atttcacctc
    21541 aaacggcatc acagtgcata cgcataagca cggtggcgtt aaaggtggca gcgattcgac
    21601 aggaggcccg cagtgacaac ccgctacaca ggaatgaatc cggacggaac gggaaacctg
    21661 aacgatatgg agcacctgaa acagtcagtc agggacatcc tgaccacccc gctggcaagc
    21721 cgggttatgc gacgggaata tggcagcctt gtgcctgatt tgattgacga acccatgaat
    21781 aacaccacgc gtctgcaatg catgagcgct gccgtgattg cactgacacg atgggaaccc
    21841 cgcattgccc tggacgccat cgacgttgtc tggaaggcag gaggccgcgc cggggtgacg
    21901 ctgtcgggca ctgtcatgca gaccatgcag aatgttgaat taaccatcac gctgagagag
    21961 taaatcatgc ctgccgttga cctttcccag ttaccggaac ccgccatcat cgcggagcct
    22021 gactttgagg caattctggc tgacacaaag gccatgatga ttgcgtccta tcccgccgaa
    22081 cagcgtgaag ccgtttccgc cgcgctggag ctggaatcgg aaccccttaa cgttatcgct
    22141 caaaccatgt cgtttcgtga aatgctgtta cgccagcggg ttaacgaggg cgcacgcgcc
    22201 tgcatgttaa gccacggttc agggacaaac ctggacaacc tcgcgggcaa tatgaacaca
    22261 aagcgcctgg ttatcactcc ggcaacggat accaccgacg cggtgatgga gagcgacacc
    22321 tcgctgagac tgcgggcgca gcgggcgtat gacggcctga gtgttgcagg cccgtcaggc
    22381 gcatacgagt attttgcacg cagcgccagc ggtctggtgc gtgatgcgcg ggccatcagc
    22441 ccgtctccgg cctgtgtgac ggtttccatc ctgtccacag agggcgacgg cacagcaacg
    22501 gaggcgttgc ttaataccgt tcgcgctgtt ctgaatgcag aggatacccg cccggtggcc
    22561 gaccgcctta ctgtacagag cgccagaatc gtgacatggc ggctgaatgc aaaactgtac
    22621 ttttaccccg gcccggaatc cgaacctatt ctggccgcgg cggaatcgtc gttcaggaag
    22681 tggctgtctg agcaggggct tatcggtcag gacgtggcgt tgtcagccat tgctgccgca
    22741 ctgcatgtgc acggtgtgca acgcgtggag ataatcgaac ccacacagaa tatggccatc
    22801 agcgacatac aggcggcgcg ctgtgagtca ttcaccatca gcgaaggtgg gcgtaatgag
    22861 taattcactg ttaccgccat cagccagcaa tttcatgcgt tgtgccgaag ctgtcggaac
    22921 gcgcattaca gacattccgg tagacctcaa cacgctgtgg tcgccggaca cctgcccggt
    22981 gcatctgctg ccttatctcg cctgggcatt ttccgttgac cgctgggatc gcaactggcc
    23041 ggaagagaca aagcgacagg ttattcgtga tgcatggctg atacaccgac acaaagggac
    23101 catcagcgca ctgcgccggg ccattgagcc gctgggatac ctcattcgtg tgtctgagtg
    23161 gtgggagttc ggcggagaac cgggaacatt taccgttgaa gtcggcacgc tggacagtgg
    23221 cgtgacggag gaaatgtatc tggaaatgga gcggttgatt gctgatgccc gcccggtcag
    23281 ccgccacatg acagggctga atatcattca ggagatcccg ggggatattt tcgcggcggc
    23341 agcaacttac gacggtgaag tcattaccat ttatccggac gattaagcat gagtaccaca
    23401 acacgaaaat ttaaaaccat tatcactgat actggtgcca aaaaattagc tcaggcagcc
    23461 gcgccagatg gtaatcctgt ccacctgact cacatggccg tgggcgacgg tggcggcacg
    23521 ttgcccacac cagacagtaa gcagacccgt ctggtgcatg aggtgtggcg acatactgtt
    23581 aatcgcgtca tcctggacgc aacacatcag aaccgcatta ttgcggagct ggttattcct
    23641 cctgaaacgg gcggattctg gatccgggaa attggtgtat ttgatgagca cggcgatttg
    23701 atcgcggtgg gcaatactgc cgaaagttac aagccagccg ttgccgaagg atccggacgt
    23761 gcacaaacat ttcgcaccat tctgaccgta tccagcactg ccaccgtggc gcttaccgtg
    23821 gataacacca tggttatggc cacagtggat tacgtgaatg acaaactgaa agaacatgaa
    23881 cagtcacgac gtcacccgga cgcctcgctg accgcaaaag gctttgttca actcagtagc
    23941 gccaccaaca gcacgtctga agcactggcc gcaacgccga aagcggtcaa ggcagcctat
    24001 gaccttgcta acgggaaata taccgcacag gacgccacta cagcgcgaaa aggcattgtc
    24061 cagctcagta gcgccaccaa cagcgattca gaaacgctgg cggcaacacc aaaggcggta
    24121 aaggcagcct atgacctggc taacgggaaa tacaccgcac aggatgcaac aacagcacag
    24181 aaagggataa tccagctaag cagcgcgacc aacagcacat ctgaaacgct tgccgcgaca
    24241 ccaaaggcag taaaaacagc ctatgacaat gctgagaaac gtctgcagaa agatcagaac
    24301 ggtgcggata tccctggcaa agacaccttt acgaaaaata ttggtgcctg ccgtgccttc
    24361 ggtgggtcag taagcacaac aacaggaaac tggacgactg cacagtttat cgagtggctg
    24421 gattctcagg gagcatttaa ccatccatac tggatgtgca agggttcctg gtcttatggc
    24481 aataataaaa tcattactga tactgactgc ggtaatattc atctcgccgg agctgtcatt
    24541 gaagtaatgg ggataaagtc agcgatgacg atccgcatta ccacaccgac cacctccact
    24601 ggtggtggaa caactaacgc ccagtttacc tatattaatc acggaacaga ttattcacct
    24661 ggctggcgaa gggactataa ctccagaaat aagccaacgg catcagagat cggggcgtta
    24721 ccgtcaggtg gaacagcagt atcatcagtt aatctggctt caaaaggtca ggtaaccgcg
    24781 ctgacagaca atacgcaggg ggcaacaggt cttgagttat acgaggtgta taacaacgga
    24841 tatccaacag cgtatggaaa tatcattcac ctgaaaggga tgacagccgt tggcgaaggt
    24901 gagttactca tcggctggag tggtacaagc ggtgctcatg ctccggcatt tattcgttca
    24961 cgacgggata cgaccgacgc aaactggtcg ccgtgggcgc agctttacac ctcggctcat
    25021 cctcctgcag agttttatcc agtcggtgca ccaatcccgt ggccatcaga taccgttccg
    25081 tctggttatg ccctgatgca ggggcagact tttgacaaat ctgcctaccc gaaacttgca
    25141 gccgcttatc cgtcaggcgt gatccctgat atgcgtggct ggacgattaa gggcaagccc
    25201 gccagtggtc gtgccgtatt gtctcaggaa caggacggca ttaaatcgca cacccacagc
    25261 gccagcgtat ccagtacgga tttgggtacg aaaaccacat cgtcgtttga ttacgggacg
    25321 aaaacaacct cactatttga ctatgggacc aaaactacca gtaacgccgg gagtcacacg
    25381 cattcaatac caacatcaga tggtgcaaac cagtggggga aaattccact gacatctggg
    25441 cgtctggcaa ctcaatatac atcagcaaca acaggttcct ctggctcaca ttctcatact
    25501 gtaggtattg gttctcatca gcacactgtc ggtattggtg cacacacgca ctccgttgcg
    25561 attggctcac atggacacac catcaccgta aacgctgctg gtaacgcgga aaacaccgtc
    25621 aaaaacatcg catttaacta tattgtgagg cttgcataat ggcattcaga atgagtaaac
    25681 aatcacgaac cctcaaaatt tataatctgc tggccggaac taatgaattt attggtgacg
    25741 gtgacgcata tattccacct catacaggtc tacctgcaaa cagtaccgat attgcaccgc
    25801 cagatattcc ggctggcttc gtggctgttt tcaacactga tgaggcatcg tggcatctcg
    25861 ttgaagacca tcggggttat accgtctatg acgtggcttc cggcgacgcg ttatttattt
    25921 ctgaacttgg cccattaccg gaaaatgtca cctggttatc cccggaaggg gagtttcaga
    25981 agtggaacgg tacatcctgg gtgaaagatg cagaagcaga aaaactgttt cggagccagg
    26041 aggcggaaga aacaaaaaac agcctgatgc aggtagctag cgagcatatt gcgccacttc
    26101 aggatgctgt agatttggat attgcgacgg aagaagaggc atcgttactg gctgcatgga
    26161 agacatatcg ggtattgttg aatcgtgttg atacaacagt agcagcggat attgagtggc
    26221 cagtcgcccc acaataaaaa gaaaaagcca tcgatagaaa tatcgatggc tttatgtgct
    26281 ctatttatac aatacaacac cgctcttttt agttatatat gtgcagttcg atggtatatc
    26341 tttatttata aaagacattg cgcctatttt tacattatcc ccgattttac gtgataatcc
    26401 aatgatgcaa caattagctc cgatatcaac gttactacca atttttactc ttgaaccagg
    26461 tatgtcacca tctatctgtc caatcgtagt attctgtcgt aataccagat tttcacctgc
    26521 atcaacagca aaatgaacaa caattccagc atgatgggga attgttaacc cttttccaat
    26581 atttgctccc aatcctattt cacaaccaaa tttgttaatt at
//
LOCUS       DAZFWP020000062        26367 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFWP020000062 DAZFWP020000000
VERSION     DAZFWP020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.062"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            61..1707
                     /gene="mqo"
                     /locus_tag="ACHK0Z_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             61..1707
                     /gene="mqo"
                     /locus_tag="ACHK0Z_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU4502388.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(1856..2365)
                     /gene="eco"
                     /locus_tag="ACHK0Z_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             complement(1856..2365)
                     /gene="eco"
                     /locus_tag="ACHK0Z_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU4502389.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2479..2643)
                     /gene="yojO"
                     /locus_tag="ACHK0Z_RS11915"
                     /old_locus_tag="pgaptmp_002383"
     CDS             complement(2479..2643)
                     /gene="yojO"
                     /locus_tag="ACHK0Z_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGU4502390.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            2752..3246
                     /gene="napF"
                     /locus_tag="ACHK0Z_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             2752..3246
                     /gene="napF"
                     /locus_tag="ACHK0Z_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU4502391.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDWQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3236..3499
                     /gene="napD"
                     /locus_tag="ACHK0Z_RS11925"
                     /old_locus_tag="pgaptmp_002385"
     CDS             3236..3499
                     /gene="napD"
                     /locus_tag="ACHK0Z_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU4502392.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3496..5982
                     /gene="napA"
                     /locus_tag="ACHK0Z_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             3496..5982
                     /gene="napA"
                     /locus_tag="ACHK0Z_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU4502393.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            5989..6684
                     /gene="napG"
                     /locus_tag="ACHK0Z_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             5989..6684
                     /gene="napG"
                     /locus_tag="ACHK0Z_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU4502394.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6671..7534
                     /gene="napH"
                     /locus_tag="ACHK0Z_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             6671..7534
                     /gene="napH"
                     /locus_tag="ACHK0Z_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU4502395.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7531..7980
                     /gene="napB"
                     /locus_tag="ACHK0Z_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             7531..7980
                     /gene="napB"
                     /locus_tag="ACHK0Z_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU4502396.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            7990..8592
                     /gene="napC"
                     /locus_tag="ACHK0Z_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             7990..8592
                     /gene="napC"
                     /locus_tag="ACHK0Z_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU4502397.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8605..9228
                     /gene="ccmA"
                     /locus_tag="ACHK0Z_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             8605..9228
                     /gene="ccmA"
                     /locus_tag="ACHK0Z_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU4502398.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9225..9887
                     /gene="ccmB"
                     /locus_tag="ACHK0Z_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             9225..9887
                     /gene="ccmB"
                     /locus_tag="ACHK0Z_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU4502399.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            9929..10666
                     /gene="ccmC"
                     /locus_tag="ACHK0Z_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             9929..10666
                     /gene="ccmC"
                     /locus_tag="ACHK0Z_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU4502400.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10663..10872
                     /gene="ccmD"
                     /locus_tag="ACHK0Z_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     CDS             10663..10872
                     /gene="ccmD"
                     /locus_tag="ACHK0Z_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU4502401.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            10869..11348
                     /gene="ccmE"
                     /locus_tag="ACHK0Z_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     CDS             10869..11348
                     /gene="ccmE"
                     /locus_tag="ACHK0Z_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU4502402.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11345..13288
                     /gene="ccmF"
                     /locus_tag="ACHK0Z_RS11980"
                     /old_locus_tag="pgaptmp_002396"
     CDS             11345..13288
                     /gene="ccmF"
                     /locus_tag="ACHK0Z_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU4502403.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13285..13842
                     /gene="dsbE"
                     /locus_tag="ACHK0Z_RS11985"
                     /old_locus_tag="pgaptmp_002397"
     CDS             13285..13842
                     /gene="dsbE"
                     /locus_tag="ACHK0Z_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU4502404.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            13839..14891
                     /gene="ccmH"
                     /locus_tag="ACHK0Z_RS11990"
                     /old_locus_tag="pgaptmp_002398"
     CDS             13839..14891
                     /gene="ccmH"
                     /locus_tag="ACHK0Z_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU4502405.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(14926..15573)
                     /gene="narP"
                     /locus_tag="ACHK0Z_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             complement(14926..15573)
                     /gene="narP"
                     /locus_tag="ACHK0Z_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU4502406.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            15893..18484
                     /gene="yejO"
                     /locus_tag="ACHK0Z_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /pseudo
     CDS             15893..18484
                     /gene="yejO"
                     /locus_tag="ACHK0Z_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(18588..18664)
                     /locus_tag="ACHK0Z_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     tRNA            complement(18588..18664)
                     /locus_tag="ACHK0Z_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18628..18630),aa:Pro,seq:ggg)
     gene            complement(18739..20499)
                     /gene="yejM"
                     /locus_tag="ACHK0Z_RS12010"
                     /old_locus_tag="pgaptmp_002402"
     CDS             complement(18739..20499)
                     /gene="yejM"
                     /locus_tag="ACHK0Z_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU4502407.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(20519..20746)
                     /gene="yejL"
                     /locus_tag="ACHK0Z_RS12015"
                     /old_locus_tag="pgaptmp_002403"
     CDS             complement(20519..20746)
                     /gene="yejL"
                     /locus_tag="ACHK0Z_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU4502408.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            20928..21935
                     /gene="yejK"
                     /locus_tag="ACHK0Z_RS12020"
                     /old_locus_tag="pgaptmp_002404"
     CDS             20928..21935
                     /gene="yejK"
                     /locus_tag="ACHK0Z_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU4502409.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(22074..22358)
                     /gene="rplY"
                     /locus_tag="ACHK0Z_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             complement(22074..22358)
                     /gene="rplY"
                     /locus_tag="ACHK0Z_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU4502410.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(22483..24243)
                     /gene="radD"
                     /locus_tag="ACHK0Z_RS12030"
                     /old_locus_tag="pgaptmp_002406"
     CDS             complement(22483..24243)
                     /gene="radD"
                     /locus_tag="ACHK0Z_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4502411.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            24392..25087
                     /gene="rsuA"
                     /locus_tag="ACHK0Z_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             24392..25087
                     /gene="rsuA"
                     /locus_tag="ACHK0Z_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU4502412.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            25115..26305
                     /gene="bcr"
                     /locus_tag="ACHK0Z_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             25115..26305
                     /gene="bcr"
                     /locus_tag="ACHK0Z_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU4502413.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
BASE COUNT         5979 a         6501 c         7437 g         6450 t
ORIGIN      
        1 ccgttgatgc cgcgcaaatc tcacctgcaa taaagcgact aaaagtaagg cattaacaag
       61 atgaaaaaag tgactgccat gctcttctcg atggccgtgg ggcttaatgc cgtttcgatg
      121 gcggcaaaag cgaaagcgtc cgaggagcag gaaactgatg tactgttgat tggcggcggc
      181 attatgagcg ccacgttggg gacctattta cgcgagctgg agcctgaatg gtcgatgacc
      241 atggtggagc gcctggaggg tgtcgcgcag gagagttcga acggctggaa taacgccgga
      301 accgggcatt ctgcactgat ggaactgaac tacaccccgc aaaacgccga tggcagcatc
      361 agtattgaaa aagcagtcgc cattaacgaa gcatttcaga tttcccgcca gttctgggcg
      421 caccaggtcg agcgcggcgt gctgcgtact ccgcgttcat ttatcaatac cgttccgcat
      481 atgagctttg tctggggcga ggataacgtt aatttcctgc gcgcccgtta cgccgcgttg
      541 caacaaagct cgctgtttcg cggtatgcgt tactctgaag atcacgcgca gatcaaagag
      601 tgggcacctt tagtgatgga agggcgcgat ccgcaacaga aagtggcagc cacgcgtacg
      661 gaaattggta ccgatgtgaa ctacggcgag atcacccgcc agttaattgc ttccttgcag
      721 aagaaatcta acttctcgct gcaactcagc agcgaagtcc gcgccctaaa gcgtaatgac
      781 gataacacct ggaccgttac cgttgccgat ctaaaaaatg gcactgcaca gaacattcgt
      841 gccaaatttg tctttatcgg cgcgggcggt gcggcgctga agctattaca ggaatcgggg
      901 attccggaag cgaaagacta cgccggtttc ccggtgggcg gacagttcct tgtttcggaa
      961 aacccggacg tggttaatca ccatctggcg aaggtttacg gtaaagcatc cgttggcgca
     1021 ccaccgatgt cggttccgca tatcgatacc cgcgttctgg acggtaaacg cgtagtgctg
     1081 tttgggccat ttgccacctt ctcaaccaaa ttcctcaaaa acggttcatt gtgggatctg
     1141 atgagttcca ccaccacctc taacgtgatg ccgatgatgc acgtcgggct ggataatttc
     1201 gatctggtga aatatctggt gagtcaggtg atgttgagtg aagaggatcg ttttgaagcg
     1261 ttgaaagagt actatccgca agcgaaaaaa gaggactggc gtttgtggca agcggggcag
     1321 cgcgtgcaga ttatcaagcg tgatgccgat aaaggtggtg tactgcgtct gggtactgaa
     1381 gtggtcagtg accagcaagg gaccattgcc gcgctgctgg gggcatcgcc aggggcgtca
     1441 accgccgcgc cgattatgtt ggatctgctg gaaaaagtat ttggcgatcg cgtttccagc
     1501 ccgcaatggc aggctacgtt gaaagcgatc gttccgtctt atggacgcaa gctgaacggt
     1561 gatgtagcgg caacagaacg cgagttgcag tacaccagcg aagtgctggg gctgaaatac
     1621 gacaagccgc aggcagcaga tagtacgcca aaaccgcagt tgaaaccgca acccgttcaa
     1681 aaagaagtgg cggatattgc gttgtaatga tgtgccacat ccggcatggt atgccggatg
     1741 tggcgtatgc tgataagacg cgccagcgtc gcatcaggca ccggctcggg cgttagatgt
     1801 cggatgcggc gtgaccgcct tattcggcct acggggtgcc gcacttcaca gcattttagc
     1861 gaactaccgc gttgtcaatt ttctcttccg ccttccagac gcggtacttc acatctacat
     1921 tgtctggcgt atacaccacg atcggcagct tgctgttgta acgcagcatt ccagcatcgc
     1981 ccagatacgc ggtgacaaat ttcttctctt tcttgccatc cgggcaggcc atcatcgtgg
     2041 aaaccggcga gctaacttta tcaaagacat aataatcata gccccagcct tccagcgttt
     2101 tgttttccag cttcccgccg agacgatgca aattgcaatc gacttccagc gtctgaccga
     2161 ttaacagttc tactttcagg gtagattcat cttcttgcgg ggttaactga atcacctgac
     2221 gcttcatccc tttttcagct tgtggataag gcgcgatttt ttccagtggc tggacgcttt
     2281 ctgccgccca ggcggaagtg gtagcgaaag cggcaaacaa tactgcaggt agaatggtct
     2341 tcattttttt gctttccttc ttcatttata ggttttcacc gatgatgtcg ctgaaggtta
     2401 acatattcat cagatttatt aatttgcttt atgaataact taaagaacat tcagacaatt
     2461 ttacctgctc ttttagtgtt aaattctaat gagagagcaa accagcagaa atatctctgg
     2521 tgaattaatc gctatataaa tatatttata accatttgaa atgtgagcaa aagcccgttt
     2581 ttgccacgct tcccgccact cttttgatcc tgctacaggt tttaccccga tcggggtatg
     2641 catctttgac acatccttta atatcttagc ggctataaaa atggcttatt aattatgcgg
     2701 tttatttggt cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa tgtgaagatt
     2761 gatgcatccc gtcggggcat actcactggt cgctggcgca aagccagtaa cggtatccgt
     2821 ccgccctggt ccggtgatga atctcatttt ctgacccatt gcacccgctg cgacgcctgt
     2881 attaatgctt gtgaaaacaa cattctgcaa cgcggcgcgg gcggctatcc gagcgttaat
     2941 ttcaaaaata acgagtgcag cttctgttac gcctgcgcgc aggcttgccc cgaatcgcta
     3001 ttttctccgc gccacaccag ggcctgggat tggcagttca ccattgggga cgcgtgcctg
     3061 gcgtatcagt ctgttgaatg ccgccgctgt caggatagct gtgagccaat ggcaattatc
     3121 tttcgcccga cgctctccgg gatctaccag ccgcaactta atagccaact ttgcaacgga
     3181 tgtggagcat gtgcagccag ctgtccggta tcagccataa ctgcggagta tctccatgca
     3241 cactaactgg caagtttgca gcctggtcgt gcaggccaaa agcgaacgaa tttcagacat
     3301 cagcacccaa ctgaacgcct ttcccggctg tgaagttgct gtcagcgacg cgccgagcgg
     3361 tcagttgatt gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa ccattgagtc
     3421 agtacgcaac gtagagggcg tgctggcggt gtcgctggtt tatcaccagc aggaagagca
     3481 aggtgaggaa acaccatgaa actcagtcgt cgtagcttta tgaaagctaa cgccgttgcg
     3541 gccgctgcgg cggctgccgg tctcagcgtg ccgggcgttg cccgcgccgt tgttggtcag
     3601 caggaagcca tcaaatggga taaagcgccg tgccgtttct gcggtactgg ttgcggcgtt
     3661 ctggtcggaa cgcagcaggg gcgtgtggtg gcctgtcagg gcgacccgga cgcaccggtt
     3721 aaccgtggcc tgaactgcat taagggctat ttcctgccca agatcatgta cggtaaagac
     3781 cgtttgacgc agccgctgct gcgtatgaaa aacggtaaat atgacaaaga aggcgaattt
     3841 accccaatca cctgggatca ggccttcgat gtgatggaag agaaattcaa aaccgccctg
     3901 aaagaaaaag gaccggaatc gatcggtatg ttcggttctg gtcagtggac tatctgggaa
     3961 ggttatgccg cgtccaagct gttcaaagcg ggcttccgtt cgaacaacat cgacccgaac
     4021 gcacgtcact gtatggcgtc ggcagtagtt ggctttatgc gtacctttgg tatggatgag
     4081 ccgatgggct gctatgacga catcgagcag gctgacgcgt ttgtgctgtg gggcgctaac
     4141 atggcggaga tgcacccgat cctctggtca cgcatcacta accgtcgtct ctctaaccag
     4201 aacgtcaccg tggcggtgct ttctacctac cagcatcgta gcttcgagct ggcggataac
     4261 ggcatcatct ttacgccgca atctgacctg gtgatcctga actacatcgc caactatatc
     4321 attcaaaaca atgcgataaa tcaggacttc ttcagcaagc acgttaacct gcgcaaaggg
     4381 gcgacggaca tcggctacgg tttacgtccg acccatccgc tggaaaaagc agcgaagaat
     4441 ccaggttctg acgcctccga accgatgagc tttgaagatt acaaagcctt cgttgccgag
     4501 tatacgctgg aaaaaactgc cgaaatgacc ggcgtgccga aagaccagtt agaacaactg
     4561 gcgcagctgt atgccgatcc gaacaagaaa gtcatctcct actggacgat gggcttcaac
     4621 cagcatactc gtggcgtgtg ggccaacaac ctggtctaca acctgcacct gctgaccggc
     4681 aaaatttccc agccgggttg cggtccgttc tccctgaccg ggcagccttc cgcgtgtggt
     4741 actgcgcgtg aagtgggcac ctttgctcac cgtctgcctg cggacatggt ggtgactaac
     4801 gagaaacacc gtgatatttg cgagaagaag tggaatatcc cgagcggcac cattccggcg
     4861 aaaatcggtc tgcacgcggt ggcacaagac cgtgcactga aagacggcaa gctgaatgtt
     4921 tactggacca tgtgtaccaa caacatgcag gccgggccga acattaatga agagcgtatg
     4981 ccgggctggc gcgatccgcg caacttcatc atcgtctccg atccgtatcc gacagtcagt
     5041 gcgctggcag ccgacttgat cctgccgacc gcaatgtggg tagagaaaga gggcgcttac
     5101 ggtaacgccg aacgccgtac tcagttctgg cgtcagcagg tacaggcgcc aggcgaagcg
     5161 aaatcggatc tctggcagtt agtccagttc tcccgccgct tcaaaactga agaagtatgg
     5221 ccggaagagc tgctggcgaa gaaaccggaa ctgcgtggca aaacgctgta cgaagttctg
     5281 tatgccaccc ccgaagtgag caaattcccg gtatccgaac tggcggaaga tcagctgaac
     5341 gatgaatccc gcgagctggg cttctatctg caaaaagggc tgttcgaaga gtacgcatgg
     5401 tttggtcgcg gtcacggtca cgatctcgcg ccgttcgatg actaccacaa agcgcgcggt
     5461 ctgcgctggc cggtggtgaa cggtaaagaa acacagtggc gttacagcga aggtaacgac
     5521 ccgtacgtga aagcgggcga aggctataag ttctacggta aaccggatgg caaagcggtg
     5581 atcttcgcac tgccgttcga accggcggcg gaagcaccgg atgaagagta cgacctgtgg
     5641 ctctctaccg gacgcgttct ggagcactgg cacaccggca gtatgactcg ccgtgtgccg
     5701 gaactgcacc gcgccttccc ggaagcggtc ttgtttattc acccgctgga tgcgaaagcg
     5761 cgcgatctgc gccgtggcga caaagtgaaa gtggtttctc gccgtggtga agtgatctcg
     5821 attgttgaaa cgcgcggtcg taaccgtccg ccacagggcc tggtgtacat gccgttcttc
     5881 gacgccgcac agctggttaa caaactgacg ctggatgcga ccgatccgct ctcgaaagag
     5941 acggacttca agaagtgcgc agtcaaactg gagaaggtgt aagccattat gtcccggtca
     6001 gcgaaacctc aaaatggtcg ccgccgcttt ctgcgcgatg tcgttcgcac agcaggcggg
     6061 ctggctgccg tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc atctggcgtg
     6121 cggttgcgcc cgcccggagc cataaacgag aacgcctttg ccagtgcctg tgtgcgttgc
     6181 ggtcagtgtg ttcaggcttg cccttacgac accttaaaac tggcgacgct ggcctctggt
     6241 ctgtcggcgg gcacgccata ttttgtcgca cgggatattc cttgcgaaat gtgtgaggac
     6301 attccgtgcg ccaaagtgtg cccaagcggt gcgctggatc gtgagattga atcgatcgac
     6361 gacgcgcgga tggggctggc ggtactggtg gaccaggaaa actgtctcaa ctttcagggg
     6421 ctgcgctgcg atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat caccctggag
     6481 ctggagcgca acacgcgtac cggtaagcac gcccgctttc tgccgacggt tcacagcgac
     6541 gcctgtaccg gttgcggtaa gtgcgaaaaa gtatgcgtgc tggaacaacc ggcaatcaag
     6601 gtgttaccgc tgtcactggc gaaaggggag ttaggtcacc attaccgctt cggttggctg
     6661 gaggggaaca atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg agaaaaaagg
     6721 ctggtggcgc agtcaccgtt ggctggtgtt acgtcgcctt tgtcagttct tcgtgctggg
     6781 gatgtttttg agcggtccat ggtttggtgt gtggatcttg cacggcaact acagcagtag
     6841 cctgttattc gacaccgttc cgctgaccga tccgctgatg acgctgcaaa gtcttgccag
     6901 tggtcatctg cctgccacgg tggcgttgac cggtgcggtg attatcaccg tgctctacgc
     6961 cctggcggga aagcgattat tttgcagctg ggtctgcccg ctgaacccga ttaccgactt
     7021 agcgaactgg ttacgcaggc gctttgacct caatcagtct gcgacgatcc ctcgccacat
     7081 tcgctacgtg ctgttggtgg tcattctgct gggatcggcc ttaactggca cgctcatctg
     7141 ggaatggatc aaccccgtat ctctgatggg gcgcagcctg gtaatgggct tcggcagcgg
     7201 cgcgctgctt attctcgcgc tgtttttatt tgatctactg gtcgttgaac acggctggtg
     7261 cgggcacatt tgtccggttg gcgcgctgta tggcgtgctg ggtagcaaag gtgtgattac
     7321 cgttgcggcc accgatcggc agaaatgtaa ccgctgtatg gattgttttc atgtttgccc
     7381 ggaaccgcat gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc aggtcaccag
     7441 ccgcgattgc atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg tatttacaat
     7501 aactacacga tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa gaaagcgctg
     7561 tgtcaatgga cggcgatgct ggccctggtg gtaagcggcg cggtttgggc ggctaatggc
     7621 gtcgatttta gccagtcgcc ggaagtatcc gggacacagg aaggggccat tcggatgcca
     7681 aaagagcagg aacggatgcc gctgaactat gtgaatcagc cgccaatgat cccgcatagc
     7741 gttgaaggtt atcaggtaac gaccaatacc aaccgctgct tgcagtgcca cggtgtcgaa
     7801 agttatcgca ccactggcgc gccgcgtatc agtcctactc actttatgga cagcgacggc
     7861 aaagtgggcg cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca cgtaccgcag
     7921 gccgataccg cgccaatcgt ggggaatacc tttaccccat caaaaggtta cgggaaataa
     7981 gaggttatta tgggaaattc tgaccgtaag cctggtctga ttaagcgcct gtggaaatgg
     8041 tggcgtaccc ccagccgtct ggcgctgggg acgctgctgt tgatcggttt tgttggcggc
     8101 atcgtcttct ggggtggctt taacaccggg atggaaaaag ccaataccga agagttctgc
     8161 attagctgcc acgaaatgcg caacacggtg tatcaggaat acatggattc cgtgcactac
     8221 aacaaccgta gcggcgtccg tgcgacctgt ccggattgtc acgttccgca cgagtttgtg
     8281 ccgaagatga tacgcaagct caaagcaagt aaagagctgt atggtaaaat ttttggcgtt
     8341 attgacacgc cgcagaaatt tgaagctcat cgtctgacga tggcacagaa tgagtggcgg
     8401 cgcatgaagg acaataactc gcaggagtgc cgtaactgtc acaacttcga gtatatggat
     8461 acaaccgccc agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa agatgggcaa
     8521 acctgtattg attgccataa agggatagcg cacaagctgc ccgatatgcg tgaagtcgag
     8581 ccaggttttt aacagggtta ttgcgtgggt atgcttgaag ccagagagtt actttgtgag
     8641 cgggatgaac gaaccttatt tagtggcttg tcatttacgc tgaacgcagg agagtgggta
     8701 caaatcaccg gtagcaacgg cgcggggaag acaacgcttc tccgtttgct gacggggttg
     8761 tctcgccctg acgcaggcga ggttctctgg caagggcagc ccttgcatca ggtacgcgac
     8821 agctaccatc aaaacctgtt atggataggc catcagccgg ggatcaaaac ccggctgacg
     8881 gcgttagaaa atctgcactt ttatcatcgc gatggcgata ccgcacaatg tctggaagcc
     8941 ctggcgcagg ccgggcttgc cggattcgaa gatattcctg taaatcagct ctcggccggg
     9001 caacaacgcc gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt atggatcctc
     9061 gacgagcctt ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca gcgtatggcg
     9121 cagcatacgg agcagggggg gattgtgatt ctgactaccc accagccgct caacgttgct
     9181 gaaagtaaaa ttcgccgcat ttcactgacg caaacgaggg ccgcatgatg ttctggcgca
     9241 ttttccgtct tgagctgcgt gtagcgtttc gccatagcgc cgaaatcgcc aacccgctgt
     9301 ggttcttcct gattgtaatt accctttttc cgctcagtat cggtccggag ccgcaactgc
     9361 tggcgcgtat tgcaccgggc attatctggg ttgctgcgct gctttcatcc ttgctggcgc
     9421 tggaacgact gttccgtgac gatttgcagg acggcagtct tgaacaattg atgttgttgc
     9481 cgttaccctt gcccgccgtt gtgctggcga aggtgatggc gcactggatg gtaaccggtc
     9541 tgccgttact cattctttcg ccactggtag caatgctact gggaatggat gtttatggct
     9601 ggcaagtgat ggcgctgacg ctgctgctgg gaacgcctac gcttggcttt ctcggtgcac
     9661 cgggcgtggc gctgacagtg ggacttaagc gcggtggtgt gctgctcagc atactggtgt
     9721 taccgctgac tatcccatta ctcatcttcg ccaccgccgc gatggacgcg gcttccatgc
     9781 atttgcccgt tgacgggtat ctggcaattt taggcgcgtt gctggcaggc accgcgacat
     9841 taagtccttt tgcgacggcg gcagcgttac gaatcagcat tcaataacgc tggattactg
     9901 atttttgttc gagtctggta tcgaaactat gtggaaaaca ctgcatcaac tggcgatccc
     9961 accacggctg tatcaaatct gtggctggtt tataccgtgg ctggcaattg ccagtgtggt
    10021 cgtgcttacc gtcggctgga tctggggatt cggctttgct ccggctgatt atcagcaggg
    10081 aaatagctac cgcattatct acctgcatgt gcctgcggcg atctggtcga tgggcattta
    10141 tgcatcaatg gcagtggcag cgtttattgg ccttgtctgg cagatgaaaa tggccaacct
    10201 ggcggtggcg gcgatggccc ccattggtgc cgtgtttacc tttattgccc tggttaccgg
    10261 ctctgcatgg ggaaaaccga tgtggggcac ctggtgggta tgggatgcac gtctgacttc
    10321 tgaactggtg ctgctttttt tgtatgtggg tgtgattgcc ctgtggcacg ccttcgacga
    10381 ccgccgtctg gcgggccgtg cggcaggtat cctggtgctg attggcgtgg tgaatctgcc
    10441 gattattcat tactccgtgg agtggtggaa caccctgcat cagggatcaa cgcggatgca
    10501 gcaaagtatc gatccggcga tgcgttcgcc gctgcgctgg tcgatttttg gcttcctgct
    10561 cctgtctgcc acgctgacgc tgatgcggat gcgtaatttg attttgctga tggaaaaacg
    10621 ccgtccgtgg gtgagtgaac tgatactgaa aagaggccgt aaatgacccc tgcatttgct
    10681 tcctggaatg aatttttcgc aatgggcggt tacgcctttt ttgtctggct ggcggtggtg
    10741 atgaccgtta ttccgctggt ggttttggtc gtgcactcgg tgatgcaaca tcgcgcaatt
    10801 ctgcgtggtg tggcgcaaca gcgggcgcgt gaggcgcgtt tacgtgctgc gcaacagcag
    10861 gaggctgcat gaatattcgc cgtaaaaacc gcttgtggat tgcctgtgcc gtgttggcag
    10921 ggctggcgct gactatcggt ctggtgctat atgcgctgcg ctcgaatatc gatctctttt
    10981 atacgccggg ggaaattctc tacggcaagc gtgaaactca gcaaatgccg gaagtcggtc
    11041 agcgtctgcg cgttggcggg atggtgatgc cgggtagtgt gcagcgcgat cccaattcgc
    11101 tgaaagtgac cttcactatt tacgatgctg aaggctcagt ggatgtctct tacgaaggca
    11161 ttttgccgga tctgttccgt gaagggcagg gcgttgtggt gcagggcgag ctggaaaaag
    11221 gcaatcatat cctcgcgaaa gaagtgctgg cgaaacacga cgaaaactac acgccgccag
    11281 aagttgagaa agcgatggaa gctaaccacc gtcgcccggc gagtgtttat aaggacccag
    11341 catcatgatg ccggaaattg gaaacgggct gctgtgcctg gcgttaggga ttgcgctgct
    11401 gctgtccgtg tatccgctat ggggcgtggc gcgcggagat gcgcgcatga tggcgtcttc
    11461 ccgcttgttt gcctggctgc tgtttatgtc tgtggctggc gcatttctgg tgctggtcaa
    11521 tgctttcgtg gtcaacgact ttaccgtcac ctatgttgcc agcaactcca atacccagct
    11581 tccggtgtgg tatcgcgtgg cggctacctg gggcgcgcat gaaggctcgc tcctgctgtg
    11641 ggtgctgctg atgagcggct ggacctttgc ggtggcgatt tttagtcagc gtattccgct
    11701 ggatattgtg gcccgcgtac tggcgataat ggggatggtc agcgtcggct ttttgctgtt
    11761 cattctcttt acctctaacc cattctcacg cacgctgccg aacttcccga ttgaagggcg
    11821 cgatcttaac ccgctgttac aggatccggg gctgatcttc catccgcctc tgctttatat
    11881 ggggtacgtg ggtttctcgg tggcgtttgc ttttgccatt gcttctttgt tgtgcgggcg
    11941 tctggacagc acttatgcgc gttttactcg tccgtggacg ctggcggcgt ggatcttctt
    12001 gacgctcggc attgtgctcg gttccgcatg ggcctattac gaactcggct ggggcggctg
    12061 gtggttctgg gatccggtag aaaacgcctc gtttatgccg tggctggtgg ggactgcgct
    12121 gatgcactca ctggcggtca ctgaacaacg cgccagcttc aaagcgtgga cattactgct
    12181 ggcaatcagt gccttctcgt tgtgtctgct ggggactttc ctggtgcgtt ccggcgtgct
    12241 ggtatcggta cacgcgtttg cctctgatcc ggcacgcggt atgtttatcc tcgcctttat
    12301 ggtactggtg attggtggtt cgctgctgct gtttgccgcg cgtggacaca aagttcgctc
    12361 acgcgtaaac aatgcgctgt ggtcgcggga atctctgctg ttagcgaaca acgttttgct
    12421 ggtcgccgcg atgctggtgg tattactggg gacgctgctg ccgctggtgc acaagcaact
    12481 gggactgggc agtatttcga ttggcgaacc gttcttcaac accatgttta cctggctgat
    12541 ggtgccgttt gcgctgctgc ttggtgtcgg tcctctggtg cgctgggggc gcgatcgccc
    12601 acgtaaaatc cgcaatttat tgattatcgc cttcatctca acgctggtgc tgtcgctgct
    12661 gctgccgtgg ctgttcgaaa gcaaagttgt ggcgatgacg gtgctcggcc tggcaatggc
    12721 ctgctggatt gctgtgctgg caattgcgga agctgcgctg cgtatttcac gtggcacgaa
    12781 aaccaccttc agttattggg gaatggtggc ggctcacctg gggctggcag tgacaattgt
    12841 tggtattgcc tttagccaga actatagcgt tgagcgtgat gtgcgcatga agtccggcga
    12901 tagcgtcgat attcatgaat atcgcttcac cttccgtgat gtcaaagagg tgactggccc
    12961 gaactggcgt ggcggtgtgg cgactatcgg cgtaacgcgc gatggcaagc cggaaacggt
    13021 gctgtatgcg gaaaaacgtt attacaacac tgccgggtcg atgatgaccg aagcggcaat
    13081 tgacggcggc atcacgcgtg acctgtacgc cgcgctcggt gaagagctgg aaaacggcgc
    13141 gtgggccgtg cgtctttact acaaaccatt tgttcgctgg atttgggcgg gcgggctgat
    13201 gatggcgttg ggcggactgc tgtgtctgtt tgatcctcgc tatcgtaagc gcgtgagtcc
    13261 gcaaaaaact gcgccggagg ccgtatgaag cgcaaagtat tgttaattcc gttgattatc
    13321 ttcctggcga ttgccgcggc gctgctgtgg cagctggcgc gtaatgccga aggggatgat
    13381 ccgaccaatc tggaatcggc gctcattggc aagcctgtgc cgaagtttcg tctcgaatca
    13441 ctggacaatc cggggcagtt ttatcaggcg gatgtgctga ctcagggcaa accagtgctg
    13501 cttaacgtct gggcgacctg gtgtccgacc tgccgtgcgg aacatcaata tctgaatcag
    13561 ctttcagcgc agggcattcg cgtggtcggc atgaactata aagacgatcg ccagaaggca
    13621 atcagctggc tgaaagagct gggcaatcct tacgcgctaa gcctgtttga tggcgacggc
    13681 atgttagggc tggatctcgg tgtctatggc gcgccagaaa ccttccttat tgacggcaac
    13741 ggtatcattc gctatcgcca tgcgggcgat ctcaatcctc gcgtctggga agaagagatc
    13801 aagccgctgt gggagaaata cagtaaggag gccgcacaat gaggttttta ttgggcgtgc
    13861 tgatgctgat gatctccggc tcagcactgg cgaccatcga cgtgttgcag tttaaagatg
    13921 aagcacagga acaacagttc cgtcagctca ctgaagaact gcgctgcccg aaatgccaga
    13981 acaacagcat tgccgattcc aactcgatga ttgccaccga cctgcgtcag aaagtgtatg
    14041 aactgatgca ggaaggtaaa agtaaaaaag agattgtcga ttatatggtg gcgcgttacg
    14101 gcaacttcgt cacttacgat ccgccgttaa cgccgctgac cgtgctgctg tgggtgcttc
    14161 cggtagtggc tattggcatt ggcggttggg tcatatacgc ccgctcgcgg cgtcgggtac
    14221 gcgtagtgcc ggaagcgttt cctgaacaaa gcgtgcagga aggtaagcgt gccggatata
    14281 ttgtttatct gccgggtatt gtggtggcgt taattgtggc tggcgtcagc tactaccaga
    14341 ctggcaatta tcagcaggtg aaaatctggc agcaggccac ggcacaggct ccggcgttgc
    14401 tggacagggc gctggatccg aaagccgatc cgctcaacga agaagagatg tcgcgtcttg
    14461 cgctggggat gcgtactcaa ctgcaaaaaa atccgggaga tatagaaggc tggattatgt
    14521 tgggccgcgt tggcatggcg ctgggtaacg ctagtatcgc caccgatgca tacgctactg
    14581 cgtatcgcct cgatccgaaa aacagcgatg ccgcactggg ttatgctgaa gcgttgacac
    14641 gttcatctga tcccaacgac aaccgcctcg gtggtgaact gctgcgccag ttggtgagaa
    14701 gcgaccacag caatatccgt gtgttaagca tgtatgcgtt taatgccttt gagcagcagc
    14761 gatttggcga agccgttgcc gcgtgggaga tgatgttgaa actcttacct gccaacgata
    14821 ctcgccgtgc ggtgattgaa cgtagtatcg cgcaggcgat gcaacatttg tcgccgcagg
    14881 agagtaaata aaaagaggcc cgatggtgat gccatcgggc tatttttatt gtgccccgcg
    14941 ttgttgcagg aacagaatgg tggctgccac gcgtgagcgg acattgagtt tgcgcagcag
    15001 attgcgaata tgtactttta ctgtctgctc tgaaatattc aacaccgagg caatctgttt
    15061 atttgacagc ccctgtgcca gctcgtgcag aacatccagc tcgcgctccg tcagcacgct
    15121 gaagggatct tcttccgcgc caaacatttc acgttcacgt aagtactgat tgacgcgttc
    15181 gctaaagact ttgctgcctt tcgctccagc acgaattgct tccagcaata cttccgggtc
    15241 gctgtctttc aacagataac cgtctgcgcc tgcgtctatc agtgcaaaga catcgctgga
    15301 ggcatctgat acggtcagga taataatttg cgcggtaacg ccatccctgc gcaaggcatt
    15361 gagggtatcc aggccactca tacctttcat attgagatcc agcaagatca cgtcgatatc
    15421 cagtctattc gccagatcga tcgcgctcgc gccgtcgccc gcttcggcga ccacttcaaa
    15481 gccaggatca agctccagta actgacgaac accgcgtcgc ataagtggat gatcatccac
    15541 aatcatcacc tgaaaaggcg ttgcttcagg catagtagtc tcctgaggtt ttattagaat
    15601 cattatgttt gagcgtagcg ttggtaacgc taccaggcgc gtgaatgtta ccccatttta
    15661 tgtatggaca tgtagtgaaa actacaacct tgtcgttcgc tttggcatca ttttcattat
    15721 aaagacggaa ttttgcatca gcttctcatc aagaaaaaca actaaaaaat caatcacaat
    15781 acttgtgcat ttctttaaac agtttttaaa aactgactcc gggtatggag ctatgggtat
    15841 tttccgtacc caatgctttt aacagcaatt aatttcatag gatgaaagct caattcatca
    15901 atctggttct gtttctcttt gtcgttccgc aatatctgtt ctgatggcta cagcgttata
    15961 ttcacccata gcattggcat caacagttga gtatggtgag acagttgatg gtgttgtcct
    16021 ggaaaaagat atccagctgg tttatgggac cgccaataat acgaaaatca atcctggcgg
    16081 agaacagcat ataaaagaat ttggtgtaag tagtaatact gaaattaaag gagggtatca
    16141 gtacattgaa atgaatggta ccgcagaata ctcagtatta aatgacggtt atcaaattgt
    16201 tcaaatgggt ggcgcggcaa accagactac cctcaataat ggtgtgctac aggtttatgg
    16261 cgcagcgaat gatcccacga ttaaaggcgg gcgcttaatc gttgaaaaag atgggattac
    16321 cgtccttgcc gctatcgaaa agggaggatt actggaggtt aaagaggggg gattagcgat
    16381 tgcggtagat cagaaagcag gcggtgctat taaagcaagc acgcgggcca tggaggtatt
    16441 cggaacaaac cgtctcggtc agttcgatat caagaatggt attgctaata atatgttgtt
    16501 ggaaaacggc ggaagtttgc gagttgaaga aaatgacttc gcttataata ccactgtaga
    16561 tagtggcggc ttactggagg ttatggatgg cgggactgta actggcgttg ataaaaaagc
    16621 aggcggaaaa ttaattgtct caacgaatgc gctggaagtg agtggtccaa acagtaaagg
    16681 ccaatttagt ataaaagatg gtgtgtcaaa aaattatgaa ctggatgatg gttccgggct
    16741 cattgttatg gaggacacgc aggccattga tactatcctt gatgagcatg ccactatgca
    16801 atcgctggga aaggatactg gtacgaaagt gcaggcaaat gcggtatatg atctcggtcg
    16861 atcagatcag aatggaagta tcacgtattc ctcaaaagcc atctctgaaa atatggttat
    16921 caacaatggc cgcgctaacg tctgggctgg cacaatggtt aacgtttcag tcagagggaa
    16981 tgatggcatt cttgaggtca tgaagccgca aataaattat gcacccgcaa tgttggtggg
    17041 taaggtagtg gtttctgagg gcgcttcttt tagaacgcat ggtgccgtgg ataccagcaa
    17101 agcggacgtt tcgctcgaaa atagcgtatg gaccatcatt gccgatatca ctacgacgaa
    17161 ccaaaacacc ctcctcaact tagccaacct tgcgatgtct gacgcaaatg tgattatgat
    17221 ggatgagcca gtgactcgtt catcagtgac ggcaagtgcg gaaaatttca ttacgttgac
    17281 caccaatacc ctgtcgggaa acggcaattt ttatatgcgt accgatatgg ctaatcatca
    17341 gagcgatcag ctcaacgtca ccggtcaggc aacaggtgat ttcaaaatat tcgtgacgga
    17401 caccggtgcc agcccggcag caggagatag ccttacactg gtaacaacgg gcggcggtga
    17461 tgctgcattt acgttgggca atgccggagg cgttgttgat atcggtacgt atgaatatac
    17521 cttgctggat aatggcaacc atagctggag tttggcagag aatcgcgcgc aaattacccc
    17581 ttcaaccact gatgtgctga atatggcggc agcacaaccg ctggtatttg atgcagaact
    17641 ggacaccgtg cgtgagcgtc ttggtagcgt aaaaggcgtt aattacgata cggcgatgtg
    17701 gagttcggca attaacaccc gcaacaacgt gaccactgat gcgggcgctg gctttgagca
    17761 aacattgacc gggctgacgc tcggtatcga tagccgtttc tcccgtgaag aaaccagcac
    17821 aattcgcggc ttgttctttg gttactctca ttctgatatt ggttttgatc gcggcggcaa
    17881 aggcaatgtc gatagctata ccctgggggc ttatgccggt tgggagcatc agaacggtgc
    17941 ctatgttgat ggagtggtga aagttgaccg ttttgccaac accatccatt gcaagatgag
    18001 taatggggca acagcgtttg gcgattacaa tagtaacggc gcgggtgctc atgtcgagag
    18061 cgggttccgt tgggttgacg gattgtggag tgttagaccc tatctggcct ttaccggctt
    18121 taccacagat ggtcaggact acacgttatc aaacggcatg cgcgcggatg tgggaaatac
    18181 ccggatatta cgcgctgaag cgggaacggc ggtaagctat cacatggacc tgcaaaacgg
    18241 tacgacgctg gaaccctgac tgaaagccgc cgtgcgtcag gaatacgccg attctaacca
    18301 ggtgaaagtt aatgacgatg gcaaatttaa taatgatgtg gctggaaccc gtggcgttta
    18361 tcaggctggg ataaggtcat cgtttacccc gacgttaagc ggtcatttgt cagtcagcta
    18421 tggcaatggc gcaggggtag aatcgccgtg gaatactcag gcgggtgtgg tctggacgtt
    18481 ctgataacaa aaaataaaca ggctgtgatg tgtcacggtc tgtttatcga attaattgca
    18541 gatataaaaa aaaccaaccg taaaggttgg ttttttcttg ggatttttgg tcggcacgag
    18601 aggatttgaa cctccgaccc ccgacacccc atgacggtgc gctaccaggc tgcgctacgt
    18661 gccgactcgt ggctgctaat actaccgttt tccacaccga ttgcaagtaa gatattttgc
    18721 taactgattt ataattaatc agttagcgat aaaacgcttc tcgtctgtca gcacttgcaa
    18781 taacaaactt aactgtggtt tttcatcttt cactctttca ccacgtaagt tgtaagtgcg
    18841 gtatttaccg ttattgttca gcaccagcgt ctttttcggg gtggtaattg ccagcgtatc
    18901 gttatccgct gcagtaaccc agtaatgacg gcgttgaggg ttgaacaaat cctgaccttg
    18961 cgaatattcg ctggcaggtg tgctgacatg tagcaggcgt tgcatcagcg tcgtcatcag
    19021 atcggtatga tcagtcagag cattaatacg ctgcgccggc gtgcctggcc agtgaatcac
    19081 taatggcacc tgcagatgac cgtgggacca gtcaaaggtt tcttcgtctt cgctcagcgg
    19141 aataccccga ccggcagtga taatcaccac cgtattgtcc agtttgccag aatcacgcag
    19201 tgcattgagc acgcggttga tctggtcatc gacattgcct gccgcccggc tatatttccg
    19261 tgcaaatgcc tgctgattgc tgtcgtcaat gttagtgcca ttgaaagaga cccacgagaa
    19321 ccagcggtta tcttcttgtg cgtagcggcc cagccagttg atccactgcg tggcggtctg
    19381 ctcgtcggat tgggtgcgta cgctcggcat cgagaaatct gacaacaatg cctggcgata
    19441 cagcgggctg gtaaagccat ctgatgagaa taaccccagc tgatagcctt gctgattaag
    19501 cgcagtaatt aatgccgcag gcgtacgggt cgacagaatg ccgtccatat agctcggcga
    19561 gatgccatag aacaggccaa agatgccgtt gtctgtagtg ttgccggagc tcatatggcg
    19621 cgtgaacgaa atattttgct cagcaaaacc tgccagcgca ggcatctgct tctcgaagcg
    19681 tgagtagttc aggccatcga cagtaatcaa cagcacattc tgcccggtgc ccatatcgcg
    19741 atagcgcagt tcgcttaacg gatactgaac ggaaacggcg tctggattac cttgctcaat
    19801 aagacggcgt tgatactcct gcgcatcaag cagaccatgc ttctcaagaa aacgtcgcgc
    19861 cgtcatcggg tacgaaagcg gcaggttagc gcgctgcatg gtgatcgggc gatagaagtt
    19921 ggcatcggcc cagatataca ccacatgcga ggcgataaag gcgataaata agaatgcggc
    19981 cagcgggcgc gcgaagcgtc gacgacgcgt caggctgcgc agcttttgcc agctccacgt
    20041 cgcaaacacc agttcaagca ataaaataac cggcacgctg atgaacatca gctgccagtc
    20101 gcgcgccatc tcattttcgt ctgggttgat aaccagttgc cagacgatgg gattaagatg
    20161 gagatggaaa cgagtaaaga cttcgctatc gatcagtaat agcgtcattc ccgccgttgc
    20221 cagaatgacg gacaaaaacc tcatcagcct ctgggagccg acgataaagg tcagcgggaa
    20281 gaggatcagc aagtaggtgg cgaacaccag gaagctgaaa tggccgataa tgcttacgta
    20341 ggaataaatg cgaccagcaa gcgttgtcgg ccagtcggcg ataaacaggt aacggctgcc
    20401 aatgacgagc gaaagcagaa tattgaacag tgcaaaccag tgcccccaac tgaccatctg
    20461 ggagactttt tcacggtagc gctgacgatg agttaccata acctgttatc tgtttccctt
    20521 agtgcgcttt gtcttcgttg atagaggact gtaaggcgcg ggcaaaagag ttggcaattg
    20581 cctggcgttg ggccggggca atgctggtgt tgataaggtt agtgaccatg ttgcctaaca
    20641 ccatcaggga aaggtcggtc ggagccttat gtttttccag tacgttgagc agctctgcga
    20701 gcaattgttc aacctgttca tcactgtagc gggaaatttg tggcataaat cgaaatcagt
    20761 tagttgatga aagggcaaca tattaccgta gcaacggttt tttttctgct tttttatgcg
    20821 ttaaatcatt ctcgttacgc tgccttctgt aaaatgaccg ccatctttta ttgcgtcgct
    20881 taaccggcgg tggttgaata ccgcccggtc ttaaaggaga gtttatcatg agtctggata
    20941 tcaaccagat tgctctgcac cagcttatca agcgtgatga gcagaacctt gagctggtgt
    21001 tgcgcgattc attgctggaa ccgacagaaa ccgtcgttga gatggtggca gaactgcatc
    21061 gggtctatag cgccaaaaat aaagcttacg gcttgtttag cgaagagagt gaactggcgc
    21121 agacgctgcg cttacagcgt cagggcgaag aggactttct ggcatttagc cgcgcggcaa
    21181 ccgggcgcct gcgtgacgaa ttagcgaaat atccttttgc tgacggtgga ttcgtgctgt
    21241 tttgccacta ccgttatctg gcggtggagt atctgctggt ggcagttctg agcaacctga
    21301 gcagtatgcg cgttaacgaa aatctcgaca tcaatccaac ccattatctt gatatcaatc
    21361 atgcggatat tgttgcgcgt atcgatttaa ccgaatggga aaccaatcca gagtccaccc
    21421 gctatctcac tttcctgaaa gggcgagtag ggcgcaaagt ggcggatttc tttatggatt
    21481 tcctcggggc cagcgaaggg ttaaacgcga aagcgcaaaa ccgtgggctg ttgcaagcgg
    21541 ttgatgattt cactgccgaa gcacaactgg ataaagcaga gcgacagaac gtgcgccagc
    21601 aggtttatag ctactgtaat gagcagctgc aggcaggcga agagatcgaa ctggagtcgc
    21661 tgtcgaaaga gctggcaggc gtcagcgaag tgagtttcac ggagtttgcg gctgaaaaag
    21721 gctacgaact ggaagagagc ttcccggcag accgtagcac actgcgtcag ttgacgaagt
    21781 ttgccggtag cggcggtggc ttgactatta actttgatgc gatgctgtta ggtgaacgca
    21841 tcttctggga tccggcaacc gataccctga ccattaaagg cacgccgccg aatttgcgtg
    21901 accaattgca gcgccggacg tctggcggca attaatcggt tttgtgttct gaatgccaga
    21961 tgcggcgtga acgccttatc tggcctgcaa aaaatagcaa taccgtaatc aggcataatc
    22021 tgcagataca aaaaaccccg ccggagcggg gttttttaca acttattcag caattaagcg
    22081 cgaacgaagt cgatgtgctg cagcttcggt ttgtacgggt gacgctgtac gtcctgagct
    22141 ttaactttga tttctttacc gtcaacaacg atggtcagaa cttcgctgta gaattcagct
    22201 ttagcttgca tgttcatgac tttgtcgtga tccagctcga tagccagcgg cgcttctttg
    22261 ccaccgtaga tgattgccgg gaacttgtta gcggcacgca ggcggcggct cgcaccctta
    22321 ccctgctctt tacgtacttc tgcgttgata gtaaacattt ctttctctct taaattaatc
    22381 ctgctacagg cgacccagca acaggtaagt gatctgcttt gcgtatgcaa aagcgggcga
    22441 gattctatac tcaatcgctt tatacatcaa tcaaaactac cattaaccgc gtaattcgtg
    22501 cgcccgacga aaacgacctt cgtaatcgaa cactttttca cgcacttgcc agtactgacc
    22561 tttcatgcgg gcaacgacaa aatccgggtg tcgcaataag gcttgctggg cgaggatatc
    22621 ggcggcggtg atccagcgca gagggatgcc cggtgtgcgc gtatgcgggc ggataaaaag
    22681 ctgctcgaag gcagttcgct gggcgggcgt ttgcagacgg aaacgctcac tcacatccgc
    22741 gccgtcttca tcgtaatagg tgattttcaa ccattcgcct ttctcgtcat gcccatgctg
    22801 taaagacatg ccgctacagc gtaataccag cgcgtctttc agtcgtagcg ccgcttttaa
    22861 catatcgtct ggatcaacca gtacggtgtc acattcgcgg cagcggcgag cggcaatatc
    22921 gttttccgcg ttacattgcg ggcaattttt aaaacggaaa cggaagtcac attgttcgcg
    22981 atgaccgtcg tcatcttcaa accatccctg acagcgacga ccaaagtgtt caatcaatgt
    23041 cccgtcggcg gtcgttttcc cccaaaaggt gttggcaaaa ccgcaggcag ggcagaaaac
    23101 ctgtaccgga acgttgtcac ttttgccttt tggtgtacca acttccggcg cgtagagatc
    23161 gtgaggatta cccgcataat caagaattaa gcaatcagtc ttgcccggag cgagacgcag
    23221 accgcgcccg acaatttgtt ggtaaagact cactgattcg gtagggcgca gaatggcgat
    23281 aagatcgacg tgcggggcgt caaatccggt ggtcagtact gcgacgttga ccagatagcg
    23341 aaaacgctgg gctttaaaat cttcaattaa cacatcgcgc tcagcgccgg gggtgtcgcc
    23401 agtaatcagt gcagcatctt cggcaggcag taatcccaca atctcttttg cgtgttcaac
    23461 ggtggcggcg aaaatcatta cccctttgcg cttttcggcg aactccatga tctggctgat
    23521 gatatgtggc gtaatgcgtt gctgtttttt cagctcacgg ttgagatcag cttcgctgaa
    23581 cagcccgtta ctctgtgcct gcaagcggct gaaatcgtat tgcactactg gcatatccag
    23641 tcgttctggc ggcgtcagat agccgtgttt aatcatataa cgcagcggca gctcataaat
    23701 gcagtcacgg aaaagggctt tctcatcgcc gcgtaccatg ccgtgataat ggaactggta
    23761 gatccagcct ttgcccaatc gaaaaggcgt ggcagtcagc cccagcaggc gtaagtgggg
    23821 attcacttta gtcaggtgag tgaggatttg ctgatactgg ctctcttcat cgtcaccaat
    23881 acggtggcat tcatcgacaa tcaacagcga aaattcaccc tgaaaggcat caagattacg
    23941 agcgaccgac tgcacgctgc caaataccac tttaccgtgg ctctctttgc gctttaaccc
    24001 agcggcaaaa atatcggctt ccagccccag cgcctgatac tttgcatggt tttgcgccac
    24061 cagttctttg acgtgtgcca gcaccagaac acgaccacgc gccagccgtg ccagttcggc
    24121 aatcaccagg cttttacctg cgccggtggg cagcacgata acggcagggg ttttatgacg
    24181 acgaaaatgg ttgagcgtgg catccacggc ttcttgctga tatgggcgga gtgtaaaaat
    24241 catggtctcg cgtaggtaga ttattctgct aatagtatgc catggatctt tttcccttga
    24301 gggatgttat tagaccgcta tactgggcag acaacagttc agattttcgg gtcaatgccc
    24361 gacctccgtt tttacaggca aaatcacaca catgcgactt gataaattta tcgcacagca
    24421 actcggcgtt agccgtgcta ttgccgggcg tgaaatccgc ggcaatcgtg tcaccgtcga
    24481 tggcgaaatt gttcgtaatg cagcgttcaa actgcttcct gaacatgatg tcgcttacga
    24541 tggcaacccg ctggcgcagc aacatggtcc acgttacttc atgctcaata agcctcaggg
    24601 ctatgtttgc tccacggacg accctgatca cccaacggtg ctctattttc tggatgaacc
    24661 ggtagcctgg aaactgcacg ccgcaggacg tctggatatc gataccaccg gtctggtgct
    24721 gatgactgat gatggtcagt ggtcgcaccg cattacttct ccgcgccatc attgcgagaa
    24781 gacctatctg gtgacactgg aatcacctgt agctgacgat acggcagagc aatttgctaa
    24841 aggcgtgcag ctgcataacg aaaaagatct cactaagcct gcggtgctgg aagtgattac
    24901 cccaacgcag gttcgtctga ccatcagcga agggcgttat catcaggtga aacgcatgtt
    24961 cgccgccgtg ggtaaccacg tggttgagct gcatcgtgaa cgtattggcg gtattacgct
    25021 ggatgctgat ttagcccccg gtgaatatcg tccgttaact gaagaagaaa ttgccagcgt
    25081 cgtctaactt ttccatgact ttcaggagcc cgttgtgacc acccgacagc attcgtcgtt
    25141 tgctattgtt tttatccttg gcctgctggc catgttgatg ccgctgtcga ttgatatgta
    25201 tctgcccgcg ctaccggtaa tttcagcgca gtttggcgta cctgcgggca gtacgcagat
    25261 gaccctcagt acttatattc tgggctttgc gttggggcag ttaatctacg ggccgatggc
    25321 agacagcttc gggcgtaagc cggtggtgct cggcggtacg ctggtgtttg ccgccgccgc
    25381 ggtggcgtgt gcgttggcaa acaccatcga tcagctgatt gtgatgcgtt tcttccacgg
    25441 gctggcggcg gctgcggcca gcgtggttat caacgctttg atgcgcgata tttacccgaa
    25501 agaagagttc tcgcggatga tgtcgtttgt catgctggtg accactattg caccgttgat
    25561 ggcaccgatt gtcggcggtt gggtgctggt gtggctgagc tggcattaca tcttctggat
    25621 cctggcatta gcggcgattc tggcttcggc aatgattttc ttcctgatta aagaaacctt
    25681 accaccggag cgtcgtcagc catttcacat tcgtaccact attggtaact ttgcggcgct
    25741 gttccgtcat aagcgtgtcc tgagttatat gctggccagc ggtttcagct ttgccggaat
    25801 gttctcgttc ttaagcgccg gaccgtttgt ctacattgaa attaaccacg tcgcgccgga
    25861 aaactttggt tattactttg cactaaacat tgttttcctg ttcgtgatga ccatttttaa
    25921 cagccgcttc gttcgccgca ttggcgcgtt aaatatgttc cgctcggggt tgtggataca
    25981 atttattatg gcagcgtgga tggtcatcag tgcgctgctg gggctgggat tctggtcgct
    26041 ggtggttggc gttgcggcgt ttgtgggctg cgtgtcgatg gtgtcatcca atgcgatggc
    26101 ggtcattctt gatgagtttc cccatatggc gggaacggca tcttcgctgg caggaacctt
    26161 ccgttttggc ataggggcaa ttgttggcgc attgctttct cttgcgacct ttaactctgc
    26221 atggccgatg atttggtcaa ttgcattctg cgcaagcagc tccattctct tctgtctgta
    26281 cgccagtcga ccgaaaaaac ggtgatctat tgcacaacga ggaagctaaa aggcttcctt
    26341 tgttgatgca tgtcaaccac aaatcta
//
LOCUS       DAZFWP020000063        26304 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFWP020000063 DAZFWP020000000
VERSION     DAZFWP020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.063"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHK0Z_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHK0Z_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HGU4502414.1"
                     /translation="MINSINSFFSSIPRSISSVTRNSSFTASQHKSTPNTVKTSSPLS
                     PSNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
     gene            complement(727..1131)
                     /locus_tag="ACHK0Z_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /pseudo
     CDS             complement(727..1131)
                     /locus_tag="ACHK0Z_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1214..1301)
                     /locus_tag="ACHK0Z_RS12055"
                     /old_locus_tag="pgaptmp_002411"
     tRNA            complement(1214..1301)
                     /locus_tag="ACHK0Z_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Ser,seq:tga)
     gene            1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHK0Z_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHK0Z_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU4502415.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHK0Z_RS12065"
                     /old_locus_tag="pgaptmp_002413"
     CDS             2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHK0Z_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU4502416.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHK0Z_RS12070"
                     /old_locus_tag="pgaptmp_002414"
     CDS             4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHK0Z_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU4502417.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLLLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHK0Z_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHK0Z_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU4502418.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHK0Z_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHK0Z_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU4502419.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMISELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHK0Z_RS12085"
                     /old_locus_tag="pgaptmp_002417"
     CDS             6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHK0Z_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU4502420.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQV
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7329..8873
                     /gene="appC"
                     /locus_tag="ACHK0Z_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             7329..8873
                     /gene="appC"
                     /locus_tag="ACHK0Z_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU4502421.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8885..10021
                     /gene="appB"
                     /locus_tag="ACHK0Z_RS12095"
                     /old_locus_tag="pgaptmp_002419"
     CDS             8885..10021
                     /gene="appB"
                     /locus_tag="ACHK0Z_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4502422.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHK0Z_RS12100"
                     /old_locus_tag="pgaptmp_002420"
     CDS             10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHK0Z_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU4502423.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10202..11500
                     /gene="appA"
                     /locus_tag="ACHK0Z_RS12105"
                     /old_locus_tag="pgaptmp_002421"
     CDS             10202..11500
                     /gene="appA"
                     /locus_tag="ACHK0Z_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU4502424.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSESELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSKLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHK0Z_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHK0Z_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU4502425.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLDWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHK0Z_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /gene_synonym="yccY"
     CDS             complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHK0Z_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU4502426.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHK0Z_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHK0Z_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU4502427.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTANADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKIFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHK0Z_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHK0Z_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4502428.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHK0Z_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHK0Z_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4502429.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLHITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGTG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHK0Z_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHK0Z_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU4502430.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLHLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNIFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19029..19334)
                     /locus_tag="ACHK0Z_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             complement(19029..19334)
                     /locus_tag="ACHK0Z_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502431.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19776..19988)
                     /gene="cspH"
                     /locus_tag="ACHK0Z_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             complement(19776..19988)
                     /gene="cspH"
                     /locus_tag="ACHK0Z_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU4502432.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20274..20486
                     /gene="cspG"
                     /locus_tag="ACHK0Z_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             20274..20486
                     /gene="cspG"
                     /locus_tag="ACHK0Z_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU4502433.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20497..20685
                     /gene="ymcF"
                     /locus_tag="ACHK0Z_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     CDS             20497..20685
                     /gene="ymcF"
                     /locus_tag="ACHK0Z_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4502434.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20660..20890
                     /gene="ymcE"
                     /locus_tag="ACHK0Z_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             20660..20890
                     /gene="ymcE"
                     /locus_tag="ACHK0Z_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU4502435.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFREM
                     AYNISYLTRGPGTNTLLACCCLLRPEEVNSEY"
     gene            20880..21053
                     /gene="gnsA"
                     /locus_tag="ACHK0Z_RS12165"
                     /old_locus_tag="pgaptmp_002433"
     CDS             20880..21053
                     /gene="gnsA"
                     /locus_tag="ACHK0Z_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4502436.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(21102..22175)
                     /gene="yccM"
                     /locus_tag="ACHK0Z_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             complement(21102..22175)
                     /gene="yccM"
                     /locus_tag="ACHK0Z_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4502437.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNNWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETANSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPTIGH"
     gene            complement(22247..24991)
                     /gene="torS"
                     /locus_tag="ACHK0Z_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             complement(22247..24991)
                     /gene="torS"
                     /locus_tag="ACHK0Z_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU4502438.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPEPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLVQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANTDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMCSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            complement(25086..26159)
                     /locus_tag="ACHK0Z_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             complement(25086..26159)
                     /locus_tag="ACHK0Z_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4502439.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSDSGRKSLIDRIKGGDAITLHVWLDRYETILTERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKH
                     YDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIDK"
     gene            complement(26137..>26304)
                     /locus_tag="ACHK0Z_RS12185"
                     /old_locus_tag="pgaptmp_002437"
     CDS             complement(26137..>26304)
                     /locus_tag="ACHK0Z_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4502440.1"
                     /translation="LETVRRWVRECRIFPPPVKDGREYLFHESAVKVDLNRPVTGSLL
                     KRIRNGKKAKS"
BASE COUNT         6209 a         6653 c         6757 g         6685 t
ORIGIN      
        1 aggcgtttgg ccacatcagg tagaggccga tgtaaagcgt ttttttctat acttgactga
       61 gtataagatg tggaacgttg aagtcctgat tccgtataag aattttttac cttaaaaata
      121 gttgttgcac tggcaggact gttgctggga gaaagaggtg agctggtttt taccgtgtta
      181 ggtgtgcttt tgtgctgtga ggcagtgaag ctactgttgc gcgtaacact tgatatactg
      241 cgaggaatgc tggaaaaaaa agaattaatg ctatttatca tatagataac cttataagaa
      301 ctttatgtga tagtctaatt tatccgtttg catgaaatct accttttgtg aaattctcgt
      361 caatatttta ctcaaaatga tcggctgtag gaataaacta tttgatatta ttgcgttttt
      421 agagttgttt tcaatcagct atagataggg cttggcgcca gaatggagaa tatttaacga
      481 gaaatagaag ctttgtatca gacgggcttc cctgaataga gaagttaaat aatcaggatg
      541 atgctaatga atatcaggaa caaggtgagt gtcttcatgt tcactgaaac agggtgtcac
      601 aaaaaatatg gctataagat gatcaatgtg atttgaaata tttataggtt tacagggggc
      661 tgggcatgca tatcttactg caatgcagaa tatgtgctgc ttggcgcagc tctttattga
      721 gaatgttcaa tcttttgttt cagtttcgtg aaggatacgg acggtttctt gtgtaatatt
      781 atacatcgcc tgtttatctt tactgcatac tcctgcaagt gttaatgcta ctccagcata
      841 aatttctgga taactatgtg taaatatatc cagtgagttt acatgtttct ctttcattcc
      901 tgcatcacaa cgaaccttat ttatacctgt cgccattgat tggggagggc tagagccttc
      961 tgcacgaaaa tagtgatgtg acagatctga gatagctttg tatccttctg cctggttcag
     1021 gcctggagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
     1081 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
     1141 ccaattggta taccaagttt ataccaagag ccaaaaaaca aaggggctac catttggtaa
     1201 ccccttgatt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     1261 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     1321 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     1381 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     1441 tttactcgtt tagcaactgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     1501 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aagtttcact
     1561 acgctttatt aacaatactt tctggcgacg tgcgccagtg cagaaggatg agctttcgtt
     1621 ttcagcatct cacgtgaagc gatggtttgc cttgctacag ggacgtcgct tgccgaccat
     1681 aagcgcccgg tgtcctgccg gtgtcgcaag gagaagagac gtgcgatatg aataacgagg
     1741 aaacatttta ccaggccatg cggcgtcagg gcgttacccg acgcagcttt ctcaaatatt
     1801 gcagtctggc tgccacgtcg ctgggattag gcgcgggaat ggcaccaaag attgcctggg
     1861 cgctggagaa caaaccgcgc attccggtgg tatggatcca cggtctggaa tgcacctgct
     1921 gtaccgaatc ttttatccgc tccgctcacc cactggcaaa ggacgtcatc ctttccctga
     1981 tttccctcga ttacgacgat actttgatgg ctgccgccgg aacccaggcg gaagaagtct
     2041 ttgaagacat catcgcgcaa tacaatggca aatatatcct cgcagtagaa ggtaatccgc
     2101 cgctgggcga gcaggggatg ttctgtatca gcagcggtcg accgtttatt gagaaactca
     2161 aacgtgccgc tgccggagcc agcgcgatta tcgcctgggg aacctgcgcg tcctggggct
     2221 gcgtgcaggc cgcgcgacct aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg
     2281 acaaacccat tatcaaagta cctggctgcc cgccgatccc ggatgtgatg agcgccatca
     2341 ttacttacat ggtgaccttt gatcgcttgc cagatgtcga cagaatgggc cgtccattga
     2401 tgttctatgg tcagcgaatc cacgataaat gctatcgccg cgcccacttc gacgccggag
     2461 agttcgtcca gagttgggat gatgacgctg cccgcaaagg ttactgtctg tacaaaatgg
     2521 gctgcaaagg gcctaccacc tataacgcct gttcctccac acgctggaat gatggcgttt
     2581 ctttcccaat ccagtctggt cacggctgcc tgggttgtgc ggaaaatggt ttctgggatc
     2641 gcggttcgtt ctacagccgc gtggtcgata ttccgcaaat gggtactcat tccaccgccg
     2701 ataccgtcgg tttaaccgcg cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg
     2761 ccagcgccgt tgaccagcgc agacgtcata accagcaacc tacagaaact gaacatcagc
     2821 caggcaatga ggataaacag gcatgagcac tcagtacgaa actcagggat acaccatcaa
     2881 taatgccgga cgccgcctgg tggtcgaccc gattacgcgc attgaaggcc acatgcgctg
     2941 cgaagtgaat attaacgatc agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt
     3001 tcgcgggctg gagatcatcc tgcaagggcg cgacccgcgc gatgcgtggg cgttcgttga
     3061 acgtatctgc ggcgtctgta ctggcgtaca cgccctggct tcggtttacg ccatcgaaga
     3121 tgccatcggt attaaagtgc cggacaacgc caatatcatc cgcaacatta tgctggcaac
     3181 gctctggtgc cacgatcatc tggtgcactt ctatcagctc gccgggatgg actggatcga
     3241 tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc gaactggcgc aaagtctctc
     3301 ctcatggccg aaatcatccc ctggctattt cttcgacgta caaaaccgcc tgaaaaaatt
     3361 tgttgaaggc gggcagttgg ggatcttccg caatggctac tgggggcacc cgcagtacaa
     3421 actgccgcca gaagccaacc tgatgggctt tgcccactat ctagaagctc tcgatttcca
     3481 gcgtgaaatt gtcaaaatcc acgcggtctt tggcggtaaa aacccgcatc caaactggat
     3541 tgtcggcggg atgccttgcg ccattaacat tgacgaaagc ggcgcggtcg gggcggtcaa
     3601 tatggaacgc ctgaacctgg tgcagtcgat tatcacccgc acggcagact tcattaacaa
     3661 cgtgatgatc cccgacgcct tagccatcgg tcagtttaac aaaccgtgga gcgaaatcgg
     3721 cacgggtctt tctgataaat gtgtcctcag ctacggcgca ttcccggata ttgccaacga
     3781 ctttggtgag aaaagtctgc tgatgcctgg cggcgcggtg attaacggcg acttcaacaa
     3841 tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag gagtttgtcg accacgcctg
     3901 gtatcgttat cccaacgatc aggtcgggcg tcatccattc gatggcatta ccgacccgtg
     3961 gtacaacccc ggcgatgtca aaggcagcga taccaacatt cagcagctga atgaacagga
     4021 acgctactcg tggatcaaag cgccgcgctg gcgcggtaac gcgatggaag tggggccgct
     4081 ggcacgcacc ttaatcgctt atcacaaagg cgatgctgcg accgttgagt cggtcgatcg
     4141 catgatgtcg gcgctgaacc tgccgctttc cggtatccag tcaacgttgg gccgcatttt
     4201 gtgccgcgcg cacgaagcac agtgggccgc aggtaagttg cagtatttct tcaacaagct
     4261 gatgaccaac ctgaaaaacg gcaatctcgc cactgcttcc acggaaaaat gggaacctgc
     4321 aacttggccg acagagtgcc gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg
     4381 ccactgggcc gccattcgcg atggcaagat tgatctctac cagtgcgtgg tgccgaccac
     4441 ctggaacgcc agcccgcgcg atcccaaagg gcagattggc gcttatgaag cagcgctgat
     4501 gaacaccaaa atggcgatcc ccgagcaacc gctggagatc ctgcgtactc tgcacagctt
     4561 tgatccgtgc ctcgcctgtt caacacacgt gctgggcgac gacggtagcg agctgatctc
     4621 cgtgcaggtg cgttaacagc gaaggagaat catcatgcaa cagaaaagcg acaacgttgt
     4681 cagccactat gtctttgaag cgccagtgcg tatctggcac tggctgacgg tgttatgcat
     4741 ggcggtgttg atggtcaccg gttactttat cggcaagccg ctaccttccg tcagcggcga
     4801 ggcgacgtat ctgttctata tgggctacat caggttaatt cacttcagcg ccgggatgat
     4861 ttttaccgtg gtcttgctga tgcggatcta ctgggctttt gttggcaatc gatactcccg
     4921 cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat
     4981 ccgctggtat ctgcttctgg caaaacgtcc gagtgccgat ataggccata accccatcgc
     5041 ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca ctggctttgc
     5101 gctgtacagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg tggtggaatt
     5161 tttctactgg acgggcggca actcaatgga cattcacagc tggcatcggc tggggatgtg
     5221 gctgattggc gcatttgtga tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc
     5281 cgacgacacg gtgatctcca ctatggtcaa cggctaccgt agccacaaat ttggcaaaat
     5341 aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg taatggggct gggcaacctg
     5401 ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac
     5461 tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt gctggggtat
     5521 gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct ggaacctgga
     5581 acgctgcgaa cctatgccgg agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc
     5641 ctgcaccaga acagtttctc cgaagtgctg gcgctggcag atatccgcgg acatctgcca
     5701 gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg acgactacgg cggcagcctg
     5761 agcgaactgg cgcgggagca actgcccgct gcggaacaat cggcgctggc gcaactggct
     5821 gcgtggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaatta tgactgtctg
     5881 tcgatggaaa attacgaagg cgttcgcttg cgccagtacc ggatgacaca ggaggagcag
     5941 ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga
     6001 cgccggtcag tgaatcccgt cttgacgact ggcttacgca agcgccagac ggcgtggtgt
     6061 tattaagcag tgacccgaaa cgtacgccag aggtcagcga taatccggta atgattagcg
     6121 aattactgcg cgagtttccc gactatacat ggcaggtggc gattgctgac cttgagcaga
     6181 gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc cgccacttta gtgtttaccg
     6241 gcggaaacta tcgcggtgtg ctgaatggta ttcacccgtg ggcggaactg ataaacctga
     6301 tgcgcgggct tgtcgaaccg cagcaggagc gtgcctcatg agcgaaactt ttttccatct
     6361 gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac tgccgatcac
     6421 ctgtcaggtg aatgatgaac cgagtatggc ggccctggag caatgtgctc acagcccgca
     6481 ggtgattgcg ctgttaaacg agttacaaca tcaactaagc gaacgccagc cgccgttggg
     6541 cgaggtgctg gcagtcgatc tgttaaatct caacgccgac gatcgtcact ttatcaatac
     6601 gcttctcggg gaaggggaag tgtcagtgcg cattcagcag gttgacgaca gtgaaagtga
     6661 aatacaggag gcgatcttct gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt
     6721 gctggaggac aaactggagg ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca
     6781 aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga tgaatggcct
     6841 accgttggcg catgagttac tggcacatgt acgtaacccc gacgcgcagc cgcacagcat
     6901 taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac gtctctgtgg
     6961 gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca acgccacggg
     7021 gttacgccat gtctggcatt tacgctgtac ggacacctta aaaggcccgt tactggaaag
     7081 ttatgaaatc tgcccaatac cggaagtggt gctggcagcg ccagaagatt tggtcgactc
     7141 tgcgcagcgg cttagcgagg tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga
     7201 cggtaagtat cgctttcagg cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga
     7261 gtttcaggat aaagagggag atctaccatt atcgggttat ttttctctct tcgcctacag
     7321 gagtgcgcat gtgggatgtc attgatttat cgcgctggca gtttgctctg accgcgctgt
     7381 atcacttttt atttgtaccc cttaccctgg ggctgatttt tttgctggct atcatggaaa
     7441 ccatttacgt ggtcaccggc aaaacgatct accgcgatat gacgcgcttc tggggtaagc
     7501 tcttcggtat caactttgct cttggcgtgg ctaccggcct gacgatggag tttcagtttg
     7561 gtactaactg gtcattctat tccaactatg tgggcgatat ttttggcgca ccactggcga
     7621 tggaagcatt aatggccttc ttcctcgaat ccacctttgt cgggctgttc ttcttcggct
     7681 ggcaacggct gaataaatac cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa
     7741 atctctctgc gttgtggata ttgaatgcca acggttggat gcaatacccg accggtgcgc
     7801 attttgatat cgacaccctg cgtatggaga tgaccagttt cagcgagttg gtctttaacc
     7861 cggtcagcca ggtgaaattt gtgcacaccg taatggcggg ctacgtgacc ggggccatgt
     7921 ttattatggc gatcagcgcc tggtatttac tgcgcggacg ggagcgcgat gtcgcattac
     7981 gctcgtttgc catcggttcc gtcttcggta ctctggcgat tatcggtacc ctgcaactcg
     8041 gagacagttc tgcgtatgaa gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag
     8101 gggagtggca aacggaacct gcacctgcac cgttccatgt ggttgcctgg ccggaacagg
     8161 atcaagagcg taacgccttt gctatcaaaa ttcccgcgct gctagggatc ctcgccactc
     8221 actcattaga taaacccgtg ccgggtctga agaatttgat ggctgaaacc tacccacgct
     8281 tgcaacgcgg acgtatggcc tggctgttaa tgcaggaaat atcgcaaggc aatcgtgagc
     8341 cgcatgtgtt gcaggcattc cgggagctgg aaggcgacct tggttacggc atgttgctct
     8401 cccgctatgc gccggatatg aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg
     8461 gcgcgatacc tcaggttgcg ccggtattct ggagtttccg catcatggtg ggctgtggtt
     8521 ccctgctgct actggtgatg ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc
     8581 agcatcgctg ggtgctgaaa atggcgctct ggagtttgcc gttgccgtgg attgcgattg
     8641 aagccgggtg gtttatgacc gagtttggtc gtcagccgtg ggcgatacag gacatcttac
     8701 cgacatactc cgcgcactcc gctttaacca caggacaact ggctttctca ctgatcatga
     8761 tcgtagggct ttacaccctg ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc
     8821 gtctggggcc gagcgcgatg cagagtgaac aaccgacgca gcaacagggg taaaggagaa
     8881 aatcatgttt gattatgaaa cattgcgctt catctggtgg ctgctgattg gcgtgatcct
     8941 ggtggtcttt atgatctccg acggatttga catggggatc ggctgtttgc tgccgctggt
     9001 ggcgcgtaat gatgatgaac gccggatagt gattaatagc gttggtgcgc actgggaagg
     9061 caatcaggtc tggttgatcc tcgcaggtgg agcattattt gccgcctggc ccagagtgta
     9121 tgcagcggcg ttttccggct tttatgtggc gatgatcctg gtgctgtgct cactgttctt
     9181 ccgcccgctg gcctttgatt atcgcggaaa aatcgccgat gcacgctggc gtaaaatgtg
     9241 ggatgccggt ctggtcatcg gcagtctggt gccgccggta gtcttcggta tcgccttcgg
     9301 caacttgctg ctcggcgtgc cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg
     9361 cagcttctgg caactgctga cgccattccc tttattgtgc ggattactca gccttgggat
     9421 ggtgattttg caaggtggcg tctggttaca actgaaaact gttggtgtga ttcatctgcg
     9481 ttcacagctg gcgactaaac gcgccgcact actggtgatg ctgtgctttt tgctggcggg
     9541 ttactggctg tgggtcggta ttgatggctt tgtactgctc gcccaggatg ctaacggtcc
     9601 ttccaatccg ttaatgaaac tggtagcagt gctacctggt gcctggatga ataatttcgt
     9661 cgaatcgccc gttttgtgga tcttcccgct gctggggttc ttctgcccat tgctgacggt
     9721 gatggcgatt tatcgtggtc gcccgggttg gggattttta atggcgtcat tgatgcaatt
     9781 tggcgtgatt ttcacggcag gcatcacgct gttccccttt gtcatgccgt caagcgtgag
     9841 tccaatctcc agcctgacgt tgtgggacag cacttccagt cagctgacgc tgagcattat
     9901 gttggtaatc gtgctgatat ttttgcccat tgtgttgctc tacactctct ggagctacta
     9961 caaaatgtgg ggacgcatga cgacagaaac tctccgccgt aacgaaaacg agctgtacta
    10021 aggagcaggg acaatgtggt atttactttg gttcgtcggc attttgttga tgtgttcgct
    10081 ctccaccctt gtgttggtat ggctggaccc gcgattgaaa agttaacgaa cgtaagcctg
    10141 atgcggcgca ttagcgtcgc atcaggcaat aatgtcggat atgaaaagcg gaaacatatc
    10201 gatgaaagcg atcttaatcc catttttatc tcttttgatt ccgttaaccc cgcaatctgc
    10261 attcgctcag agtgagtcgg agctgaagct ggaaagtgtg gtgattgtca gtcgtcatgg
    10321 tgtgcgtgcc ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    10381 aacctggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcttatct
    10441 cggacattac caacgccagc gtctggtggc tgacggattg ctggcgaaaa agggctgccc
    10501 gcagcctggt caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    10561 agccttcgcc gccgggctgg cacctgactg tgcaataacc gtacataccc aggcagatac
    10621 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataacgc
    10681 gaacgtgact gacgcgatcc tcagcagggc aggagggtca attgctgact ttaccgggca
    10741 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat tttccgcaat caaaattgtg
    10801 ccttaaccgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    10861 caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac
    10921 ggagatattt ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac
    10981 cgattcacac cagtggaaca ccttgctaag cctgcataac gcgcagtttt atttgctgca
    11041 acgcacacca gaggttgccc gcagtcgcgc caccccgttg ttggatttga tcatgacagc
    11101 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct
    11161 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    11221 gacgcttcca ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg
    11281 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    11341 gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    11401 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggccg gttttacgca
    11461 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    11521 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    11581 aaggcgttca cgccgcatcc ggtcacttta ccttcagttt tactctttct cggagtaact
    11641 ataaccgtaa tagttatagc cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt
    11701 gaggattgcg cctttaatat taacgccagc ctgttccaga cgctgcattg acaagctcac
    11761 ctctttggcg gtgttcaggc caaaacgcgc aaccagcagg ctggtgccga cagagcgtcc
    11821 cacgaccgca gcatcgctca ccgccagcat cggcggcgta tcgacaatca ccagatcgta
    11881 atggtcgttc gcccaatcca gtaattgacg catccgatcg cgcatcagca gttcagacgg
    11941 gttaggtggc acctgaccgc gagtaatcac atcaaagcct cctttgccaa aatgctggat
    12001 cactttgttg agctcatctt tacctgccag atattccgac aagccatgtt cattactcac
    12061 tgtaaacagg ttatgcgaat aaccgcggcg taagtcggca tcaataaata acactttttg
    12121 atcggactgg gcgatcaccg ctgccagagt tgaactgaca aacgttttac cactgtctgg
    12181 cgtcgcaccg gtgatcatca gaatgttatt ctccgtctcc atcatagcga agtgcagact
    12241 ggttcgtagc gcacgtacgg cttccacagc agaatccgcc gggttatcca cagccaggaa
    12301 ggggatattt ttagtacgat ggcgctgctg attagaaaat aaatttttct tacgcagacg
    12361 ggtacgttta tccagccact cggacattgg gatagtggca taaacgctga tgccgtgctc
    12421 ttccagttgt tccggggctt ctacaccacg acgcaacatc gcacgcgcca gcacggcacc
    12481 caccgaaata aacaggccaa gaataaaacc aagcaccaca ttcaacgctt ttttcggttt
    12541 cactggctgc ggctgagtga ctgccgggtc gataatccgc acgttaccaa tggcactgga
    12601 tttcgaaata ctcaactcct gctggcggtt aagtaattgc agatataccg cacggcccgc
    12661 ttctacgtca cgacttaaac gcaacacttc ctgttgggtg gaaggcatag ccgatacccg
    12721 cttattcagg cgtttgcgtt cttgctccag cgtctggcgt ttttccagca gcgcacgata
    12781 agttgggtga tctttcttat acagctggga gatctctgcc tcgcggaaag tcagctcatt
    12841 gagttgatta tcaacgttca caatctgctc aagaacggct ttggcttcca ggttaaggtc
    12901 aaccgaatcg cgctgctggc gataaacgtt gagtttttct tccgcctggt ccagctcgct
    12961 gcgcacttca ggcaactggc gctgtaagaa ttcaaggctt tgtgaatcct gcgccgcctg
    13021 gcgagcgata ttctgttgca aatagttgtt agcgatgctg ttcagaatac gagtaatcaa
    13081 ctggggatca tcaccggtca tggtaagttc cagcatcccg ctttctttac tgcgttcgct
    13141 aacggtaaag gtttcctgca acgcgttaat cgcttccagt tcggtacgtt ggctcaggac
    13201 aaactgtgtt cctggtttgg ccttaatgtc cgcgatagtc agcgcaacgc catctttttc
    13261 cagacgctgg ccgaccatac cattgacggt gaactcttca ccttccagcg tatagtggcc
    13321 gttttcccca accgtgagtg tcagttgctg atcctgacca ttcagttgtg gaatatgcat
    13381 ccagctgatc gccagctcac ctggtttctc tttggttaat ctcgcccagc cgcgacccac
    13441 aatcggaaaa tacttttgct caaccatgtc acgcagattc agttcagcaa tggttttacc
    13501 gagaatcatg cgcgattgca gcagttggat ctccggtgca gactcgggcg atgagttagg
    13561 gatcatatcg ctcaggccgc tgagaatggc gttgccctgt ttttgctcaa cctggaccag
    13621 agtatctgcc tgataaattg gtgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa
    13681 taacgcggtc acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag
    13741 cagatcgatc tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat
    13801 cgtattttat aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct
    13861 ccaacatacc gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct
    13921 ctttctgttc cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcgattg
    13981 cggtaacctg agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca
    14041 tctcagcagt gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa
    14101 catctgccgc tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga
    14161 cttttacgcc cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt
    14221 tgccggtaca aaccaccagg attgagttaa attttagttg ggccatgttt gaatccaccg
    14281 tgaagtctct gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg
    14341 gttccaacga gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa
    14401 ttccgttccg agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat
    14461 ttttccattc tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac
    14521 atcctgattc ataccctcag cgttacccag cgcttccgcc agggtcatac cgctgcggtc
    14581 cattttcagc gtgctctgtt tacccacttc acccatcacg aagattttca gatcatcgtt
    14641 gcgcggaata aataaaatat cgcccgggtg cagcagtttg ttctgacgca agtcgccacg
    14701 ctgcatcagc gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacatt
    14761 gcgccagtct gcatttgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag
    14821 aggaatattg gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt
    14881 ttgcgatcgg aacgctgcga cgctgacatc gacctgcggg ctttcaatga ctgaatcgag
    14941 acgcgctgta atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc
    15001 aatatagggg tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct
    15061 acggtattgc cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac
    15121 atcaccaatg ccgatacgat attcatagtt cgcgatttgc tgctccagct ccgggttagc
    15181 ctgcgacata atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat
    15241 attgaccatt ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact
    15301 ggtcgataaa ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt
    15361 cagtaccgaa aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat
    15421 gttatgaatt acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggata
    15481 gagctggtat ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac
    15541 caccgcgcgg ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa
    15601 acctttacta aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca
    15661 tacccctacc gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg cccctttatc
    15721 ttttgccaga tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt
    15781 ccagtaagcc gtcacgaaac ctgttggcgt ggaataatcg gtgaagcgca tcatgttgtc
    15841 ccagtcacgt tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg
    15901 gcgataaagc agctcggaac cgacaccggc gtacatcgtt tccagataac cgccatacac
    15961 ctggccatag aaaccattgc ctaagtcggc aaagtagttc gcctgcaagt tgttgagata
    16021 aacgtcattg cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc
    16081 gggtaatagc gaagacttaa acttgtcgta gttattgtaa atattggtga aaataccgcc
    16141 atcaagcagc aggtggtcag taaaccagta gcgggcgctg gacatcagcc ccaactggaa
    16201 catatagaaa tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct
    16261 aaagcgatct tcacgaatac ggtagccccg accaaaggca gaaagatctt cagcttcaac
    16321 acgttgttgt tgcagtggct ctgattgacc aggcgctgta ccggccagct gtttgcgcag
    16381 gctggctacg tcggtttcgg tagtcaccat agccatatgc tcgcgctttt gcgtcacgct
    16441 aatcttctca acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc
    16501 ttcacgagag tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag
    16561 ctgaatttct ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac
    16621 cgtggtgtac tgcaatcctt cagattcagg cgcaggttga tatgccggtt ttggcgtatc
    16681 gcgcagggca gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt
    16741 gttaccgcgt tcataactca ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc
    16801 gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc
    16861 gtcgtattcg agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa
    16921 gatggaagcc gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc
    16981 tgcgcgatga caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt
    17041 accggcgtag ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc
    17101 caccagatac tcaccgtcaa acagacccgt accagcaata tcacgtttac caaacgctac
    17161 ttgcggtagc cagtaacctt cttcccatag gcgaagttta aaatcgaaag atttgtcttt
    17221 gtatgactga tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt
    17281 ataacgaatg gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaacggta
    17341 ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg
    17401 catctgcaac aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta
    17461 ggttaatact tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata
    17521 agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt
    17581 aaatcagcat atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca
    17641 ggcggtggtt ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc
    17701 tcaggcgtaa tcaccataac gttattcttg tccgcgcctg caaggcgggg gtgatcttgc
    17761 agataatcgg taacgctacg gcctgcctgc cagggcaatt gcccagtccc agaaaccgca
    17821 cccagcagag taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccagc
    17881 gggggattgc tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga
    17941 ccagtgatat ggagattgag taactgctgg cgcacggatt taatcgttgc tgccacatcg
    18001 tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc
    18061 gctttagctt tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc
    18121 agttgcggtt gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt
    18181 tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg
    18241 taaagtacgc tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg
    18301 tcatttctac gggaataacc cctgcgccta acatttgtcg actttgacga acctggccag
    18361 tagccgaatc gacccagaat atgttttgcc actgtttttc tggcagcgtg gaagtcacct
    18421 cttcctgcca gacattacac agcacatcgc gacccgcaat attcagcgtc tctaatccag
    18481 caaaggaaaa agtggaactc agggtcgcag agcgaaaatg gttgtcttca ctccagagaa
    18541 tatcgcgcgt ccagcggctg ccgtctttta ttgccagcgc gttacgcaga gggtcctgcc
    18601 cggaatttgt cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat
    18661 tatgggtaac cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat
    18721 atcccagtac cacaaagatt cgttgccctt cattaaggtg taaatacatc gtggaataag
    18781 gcaacgcctg gatctgctga tccgctacgg tgatatcctg attatcgaaa aggctggcgc
    18841 gaaatgtatc gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa
    18901 tcgataaaat aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc
    18961 tctaaagccc ggtacgaata tcgcaaccgg gtattattta actttaattt cgacgcgttt
    19021 aagcagaatt agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca
    19081 ctgtcatttg ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca
    19141 actgtcgccg tgccgacagc gccagcagcc acgccaccag tggtagcagc cgtcgtggtt
    19201 gcaccggtag tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct
    19261 gcttcagggg cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa
    19321 agcttgtgtt tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc
    19381 aggattgtct actttgagta taacatcctt gtgaaatctc cctttcctca taccgcttgc
    19441 actatataca gcgggtgaga aaggcgcgca gagggaaata taagattgtt aacgatttcc
    19501 ctctggaaat acgacttgtg agcataagat aaatcttaga aaaatacaaa ttaaagataa
    19561 gaaaattgtt aagaaagcta ttaactattg ttgataatat agtttcaagc ggtactataa
    19621 ctggctgtta agtggcattt ataactatta gttgcagaga tattcggtta aatggattag
    19681 tttcctgata aaaaagtaac ttgcgttata atgcatggtg ttgactattg aactattcca
    19741 ataaaaccag tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct
    19801 gttggtcctc gcaggccatt tacgcgacaa aattccacgc gtaatcctgg tataagcact
    19861 tctgcgtcgc ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag
    19921 gggataatga atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta
    19981 cgggacaaac aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata
    20041 aagccagcga aatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat
    20101 catgcgttaa tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag
    20161 cagttctcat tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtag
    20221 tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta
    20281 ataaaatgac tggtttagta aaatggttta acgcagataa aggttttggc tttatcactc
    20341 ctgatgatgg cagcaaagac gttttcgtcc atttcaccgc catccagagc aatgaattcc
    20401 gcacgctgaa cgaaaatcag aaagttgaat tttctattga gcaggggcaa cgtggccccg
    20461 cggcagcgaa cgttgttacg ctctaaggtt gccgttatta ctcaacatct ccatttccgc
    20521 tgtccatgtt gtcatggttc acagtaccgc acatcggcat tcgatgtgac ggagcgaaac
    20581 cctttgggcg ctaagtgtat tttttgtaaa tcgacaatga tcacctttga taacgtcgcg
    20641 ctgcaaatac gcactgacca tgcgccgctg gatttcacaa aataatatca ggctccctcg
    20701 tggagccttt tttatatctg ccttattttt cttcaacgct gtatgtatag taagcgataa
    20761 cctgttgatt attgaatctt tcagggagat ggcttataac atttcttacc tgaccagggg
    20821 accgggaacc aacaccttac tggcgtgttg ctgtctttta agaccagaag aggttaacag
    20881 tgaatattga agagttaaaa aaacaagccg aaacggaaat cgccgacttt atcgcgcaaa
    20941 aaatcgccga gctgaacaag aatacaggga aagaagtctc tgaaattcgc ttcaccgcac
    21001 gggaaaaaat gaccgggctt gaaagttatg atgtcaaaat caaaataatg tgattttgtg
    21061 aacatcaccc cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc aatcgttggc
    21121 gcttgtggaa ttaagcgtcg gtacaattcc tccggcaccg ggctttgcca tactcccgca
    21181 tacattgcgt aaccaatcac cgcaaacata atccccagaa ccagtagtgt cattaaccag
    21241 ccagacaacg caaaagcttt tttatttgcc gcaggttttt gcagtgaaaa ggtcaatgtt
    21301 gaggctaccg gacatgactc tacgcaagtc atacagccgg tacattccac tgttcgtacc
    21361 tgaattaatt tatcgaccgg gatccgtgat gggcaatttt ttgcgcattt gccacagtcg
    21421 atacaacttt cggcattgcg acgaatctta aacggcgata atagcgaaac cacgcccatc
    21481 agcgcgccat atgggcaaag ataacgacac caggcatggc gaataaacag gctggcaatc
    21541 agcaaaacgg tcacgctgat taatgtcgcg gtccccatat gacgaaagaa atcgagcatt
    21601 ttaacgtcca tcaccacgct gtagggcgac aacataaaat agtgaatcgc ctgagcgggc
    21661 atcaataacg cgatatagag aaaaaaactc aacagcaaat acttcacgcc gcgcagagga
    21721 atatccagcc agcggggaag gacacattgc cgaccaaaca gtttgttacc gagatcgccg
    21781 attaattcag aaagcgtacc aaccgggcat aaccatgagc aaaaggcctt tttgagtaat
    21841 agactgatga cgataaaagc gaccaataac agcatcgcgg cggcgtggac ggacggtaac
    21901 tgacctgtgg taaggctata tttcagattc atcagaccgg caatcggtag ccagccttcg
    21961 atacctcccg gtctggcgac aaatgtcgtg ctatttgccg tttcgtaata gcgcacccaa
    22021 taccagaacg tgatggcaat ataaatattc attgccaaca gtaataattg cgtcgcttta
    22081 cgccaggtcg tggcattgcg ccagttattc cacggtaatt tgccgcccgt cgtgcctggc
    22141 cgccgctgcc agcgggttct tttattctct gccatgattt tgccagtccg ttaagttgca
    22201 taccaaatgc cactattcta gttgttctta actggctgat attgattcaa atcgcgttca
    22261 ggtctttctt atgcaaccat gcttccagag cggcaacact gcgtgtaatt tcttcgtgtg
    22321 gaaggggggc agataatggc tgctgctcca gttgtgcgca tagctggctg gcactgcaca
    22381 ttcccagact cgagcaactg ctttttagct gatgtgcggc acgctttatt ttctcgctat
    22441 cctgactggc gcgggcaatg tcgatttcat cgagaagcgg cagggcatgt tgtgtaaata
    22501 ataccagcca ttcgtggatc ttctccgtcc ccattaactg agcatcttca ttgagttgcg
    22561 atacatccag cggttgatcg gtattggctt gcagttggag atagtgcgcc agtaactgac
    22621 cgagcacttc acgcggcacc ggtttaggga taatcccgcg aaataatgaa ctggtacgct
    22681 ggcgcaacgt ttcgtcgatg acatgagcgc taaagccaat caaaaccagc gacggatatt
    22741 gctgtaccag ttgtcgggca agggtaatgc cgtcgatatc cggcagatca aaatccacca
    22801 gtgcggcagc aaacggttcg ctattttgca gtgtctctaa agcctgcgcg gcattgccaa
    22861 cagcaacaac ctgcgcacca ctggtgttca gcatctcaac ggtaattcgc tgggttagcg
    22921 ggttatcttc aattaacagc aaacgtaaac cgtccagacg caccgcctga ttggctgttt
    22981 ttggctcggg tgcagtggca acacgtaacg gcaagcgtaa acaaaaacag cttccaacct
    23041 ccggcgtgct ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg ctactgatag
    23101 tcagccccag cccggtgccg ccgcgtttgc cgcttacctg cacaaatggc tggaagattt
    23161 ctgccagttt cgcgggatca ataccgcagc cgctgtcttc cacttcgacc agccattgct
    23221 cgccatcagt gcggctacgt aaaacgatct gcccttcgtc agtaaaacgt agggcgttgc
    23281 tcaacaggtt ggttataacc tgacgaatac gtcgcggatc gcccattaac gcgctcggta
    23341 catcatcggc aattgccgtt gccaggcgaa tcgggcgacc tttcacccgt ccgctcatta
    23401 attgcagggt actttccagc agcgggcgcg gttcaaaggg ttcatcgctg accgaaacat
    23461 tcttgccgcc tgcttcgata gccgaataat cgagaatatc gttcaggatg gtcagcaacg
    23521 attcaccaga gtcagtaatt gcccgcaaat catcacgctg ggcgttaagt gcggggttat
    23581 ctgccagtag ctgtgcggtg ccgagaatac cgtacagcgg tgtgcggatc tcatggctca
    23641 tcgccgccag aaacgccgat tttgcctggc tggctttttc tgcttccgcc cgtgcctggc
    23701 ggtgttctat caccagttcc tgcaattcag ctgtacgcgc tttgacctgc gccgccagct
    23761 gttcgcggtg gcgattcagt gcatgaacat tgctgcgaaa cgcatccatc agccgcccga
    23821 tggtatccag ctcccgtacg ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca
    23881 gcagccgttg cagcgcctgc gtttgttcgg caagtggacg cgtgactgag cgataaacca
    23941 cgcgccaaag gatcagaatc agtgcgcaaa gtgaaaccat ccccagcaat aacaggctgt
    24001 attgcccgcg tgcactggct ttttccagat gcgccagtcc gtgctgatta cgcagctcaa
    24061 tggtgtcgac cagctgactg acttcgctac taaactgcgc gaactgggcg atgttatttt
    24121 gtgcgagagt ttgtaggtga ttgctgattt cactgtcctg ctgatacagc gccagcaaat
    24181 cgctatattg gctaacggta gttaacgttg ttgcgacctg cgcacgaaca cccggatctt
    24241 caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt attgagctgc ttttccagcg
    24301 ttggtgcatt tttctggatc tgctccagcc ccagattcat caccatttgc tgcacccgca
    24361 gagcgctaag gcgcagttca ttcatctggt taacatactc aagatcgata tcaatcagcc
    24421 gatcgagtgc actttcagca gcctgacgct gatcttgttc gatcaaatcg taaatcccgg
    24481 cctgggtcgc tccagcggaa gttgtcgcat tattcgcctg accttgcgcc aggcgtgcga
    24541 tctcatcggc ggcagcgact atctgctgac tgagttgccg ttgttgctgg cgtagttgca
    24601 gacgccgccc caccagttcc ccttgctgac gtaatgaacg ggagatctcc tgctcctgtt
    24661 gttcaatagc ggtggtatca aaaccttgtt cccgtaacgc ttgcagcaac gcattaatct
    24721 tcaggctttg tgcggtgagc attcgcccct gcgcctgcca catcttttcg ttatcggcac
    24781 tggtcaggtt ctgcgcggcg aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca
    24841 tattcatggt aggaatcaat gcctgagtgt tgtctttttc cacctggctg ataaagcgca
    24901 ggttgtacca tcccaccagg gtactggtca gggttaacag cgccatcagg gcaaagccca
    24961 tccagagtct tcgggtcagg gttaaattca cggtcggtgc acttaaggtg aataagttgt
    25021 atgtttaaaa tctcttttac tatcaatgaa ttagtattgg caggtcatta ttggtcaaaa
    25081 taaaatcatt tgtcgatttc aattttgtcc cattcccgtc cacggctgtc tctataccgc
    25141 gccgccattg aatctgattt atgcccgaga agacgttgag caaatttatc gccaatctgg
    25201 ttccggtata gcctcgctga caggctacgc agttcatgga atgttggcgg gtttccatca
    25261 aatgagagtc cagatgcatt tctcgccttt gtaaaatact ttgatactgt tttcggggaa
    25321 agaggatcgt aatgctttga tgcgattata gtttcactgc tgctggcctc cctgcatttc
    25381 tgtagtgtat cagccaatga gatattgagc gcgtcaatcg ttagcgttag cggaatggcg
    25441 agtttagccc ctgttttact ctgttcaatg tgaagatggt tgtcgtttat gtctgaccat
    25501 ttcattctgc acaaatcgcc gactctctgc cctgtaacga cggccaaatc catcgccagc
    25561 cttagccaga tagggagagg ttcggctgca tggtaaatct cgacatactc attagctgtc
    25621 agccttgagc gccttacttc tgactttgct gtacgggttg ctgttaccgg attcgttgcc
    25681 acatgcccct cggctattgc ttcacgaaaa acgtcaacaa gggttgacct gattaatttt
    25741 gcggaagctg ctttaccttc tgctacgtag gtgtttagca ttgctgccac ttctttcgtt
    25801 gatatgtcag tgagcggttt gtccggcaat tttcttcgga ttgccctgat tttgctggcg
    25861 tagtcgagta gagttttcgg cctgatcccc ctttcggtga ggattgtttc atatcggtca
    25921 agccacacat gaagagtgat tgcgtcaccg cctttaattc tgtctattag tgatttgcgt
    25981 ccgctgtctg agagtaactc aatattggcc tgtattgctt cagtgattgc tatcctcctg
    26041 tctcggccta aaccaaactc tttacccgtc cttgggtccc tgtagcagta atatccattg
    26101 tttcttatat aaaggttagg gggtaaatcc cggcgctcat gacttcgcct tcttcccatt
    26161 tctgatcctc ttcaaaaggc tacctgttac tggtcgattt aagtcaacct ttaccgctga
    26221 ttcgtggaac agatactctc ttccatcctt aaccggagga gggaatatcc tgcattcgcg
    26281 cacccatcga cgaactgttt caag
//
LOCUS       DAZFWP020000064        26199 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFWP020000064 DAZFWP020000000
VERSION     DAZFWP020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.064"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(41..763)
                     /gene="ubiG"
                     /locus_tag="ACHK0Z_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             complement(41..763)
                     /gene="ubiG"
                     /locus_tag="ACHK0Z_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU4502441.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            910..3537
                     /gene="gyrA"
                     /locus_tag="ACHK0Z_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     CDS             910..3537
                     /gene="gyrA"
                     /locus_tag="ACHK0Z_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU4502442.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            3686..5374
                     /gene="yfaA"
                     /locus_tag="ACHK0Z_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     CDS             3686..5374
                     /gene="yfaA"
                     /locus_tag="ACHK0Z_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU4502443.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            5371..5994
                     /gene="yfaT"
                     /locus_tag="ACHK0Z_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     CDS             5371..5994
                     /gene="yfaT"
                     /locus_tag="ACHK0Z_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU4502444.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            6018..10532
                     /locus_tag="ACHK0Z_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     CDS             6018..10532
                     /locus_tag="ACHK0Z_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU4502445.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKNEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            10533..12182
                     /gene="yfaQ"
                     /locus_tag="ACHK0Z_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             10533..12182
                     /gene="yfaQ"
                     /locus_tag="ACHK0Z_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HGU4502446.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            12187..12963
                     /gene="yfaP"
                     /locus_tag="ACHK0Z_RS12220"
                     /old_locus_tag="pgaptmp_002444"
     CDS             12187..12963
                     /gene="yfaP"
                     /locus_tag="ACHK0Z_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU4502447.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            13238..16087
                     /gene="rcsC"
                     /locus_tag="ACHK0Z_RS12225"
                     /old_locus_tag="pgaptmp_002445"
     CDS             13238..16087
                     /gene="rcsC"
                     /locus_tag="ACHK0Z_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU4502448.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(16287..16937)
                     /gene="rcsB"
                     /locus_tag="ACHK0Z_RS12230"
                     /old_locus_tag="pgaptmp_002446"
     CDS             complement(16287..16937)
                     /gene="rcsB"
                     /locus_tag="ACHK0Z_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU4502449.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(16954..19626)
                     /gene="rcsD"
                     /locus_tag="ACHK0Z_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             complement(16954..19626)
                     /gene="rcsD"
                     /locus_tag="ACHK0Z_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU4502450.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            20364..21467
                     /gene="ompC"
                     /locus_tag="ACHK0Z_RS12240"
                     /old_locus_tag="pgaptmp_002448"
     CDS             20364..21467
                     /gene="ompC"
                     /locus_tag="ACHK0Z_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4502451.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            21579..22634
                     /gene="apbE"
                     /locus_tag="ACHK0Z_RS12245"
                     /old_locus_tag="pgaptmp_002449"
     CDS             21579..22634
                     /gene="apbE"
                     /locus_tag="ACHK0Z_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU4502452.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSTLMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            22708..23772
                     /gene="ada"
                     /locus_tag="ACHK0Z_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             22708..23772
                     /gene="ada"
                     /locus_tag="ACHK0Z_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU4502453.1"
                     /translation="MKKATCLTDDQCWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVVMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRSLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            23772..24422
                     /gene="alkB"
                     /locus_tag="ACHK0Z_RS12255"
                     /old_locus_tag="pgaptmp_002451"
     CDS             23772..24422
                     /gene="alkB"
                     /locus_tag="ACHK0Z_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU4502454.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            24498..26141
                     /gene="yojI"
                     /locus_tag="ACHK0Z_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             24498..26141
                     /gene="yojI"
                     /locus_tag="ACHK0Z_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU4502455.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKFELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         6323 a         6489 c         7075 g         6312 t
ORIGIN      
        1 caagcgtcgc atcaggcatt ggctcggcca aacaacaacc tcacttattc tgcgtgtgca
       61 gcatatagtt cacatccacg ccggggccga gtttaaaagt attagtgatc gggttgtaat
      121 gtagcccggt tatatgtcgc tctttaaggc ttgtctgatc tacccagccc agcaattctg
      181 ccggtttaat aaacttcttc acatcatgcg tgcctttggg caccatgcgc aaaatatatt
      241 ccgcaccaac caccgccatc agccatgact tgccgttgcg gttaagtgtc gagaaaaaga
      301 catcgccgcc cggtttcacc agttgcgcac aggctctgac cactgactgc ggatcgggga
      361 cgtgctccag catctccatg caggtcacca catcatactg cccggcatgt tttgccgcgt
      421 gctcttccac ggtttcctgc acgtaatcca cctgaatgcc gctttccagt gcgtgcagtt
      481 ttgccacctg caatggctca aagcccatat ccagaccggt caccgtcgcg ccttcgcgcg
      541 ccatactctc ggccagaatg ccgccgccac aaccgacatc gagcaccttt ttgccaaata
      601 aaccgccagc acgctcggca atatagccca gacgcagcgg gttaatgcgg tgcagcggtt
      661 tgaactcacc ttccagatcc caccagcggg aggcgacggc ttcaaattta gcgatctctt
      721 cgtggtctac gttatggttt accggcgatt tttcggcatt cattggcact tctactccgt
      781 aattggcaag acaaacgagt atatcaggca ttggatgtga ataaagcgta taggtttacc
      841 tcaaactgcg cggctgtgtt ataatttgcg acctttgaat ccgggataca gtagagggat
      901 agcggttaga tgagcgacct tgcgagagaa attacaccgg tcaacattga ggaagagctg
      961 aagagctcct atctggatta tgcgatgtcg gtcattgttg gccgtgcgct gccagatgtc
     1021 cgagatggcc tgaagccggt acaccgtcgc gtactttacg ccatgaacgt actaggcaat
     1081 gactggaaca aagcctataa aaaatctgcc cgtgtcgttg gtgacgtaat cggtaaatac
     1141 catccccatg gtgactcggc ggtttatgac acgatcgtcc gtatggcgca gccattctcg
     1201 ctgcgttaca tgctggtaga cggtcagggt aacttcggtt ccatcgacgg cgactctgcg
     1261 gcggcaatgc gttatacgga aatccgtctg gcgaaaattg cccatgaact gatggccgat
     1321 ctcgaaaaag agacggtcga tttcgttgat aactatgacg gcacggaaaa aattcccgac
     1381 gtcatgccaa ccaaaattcc taacctgctg gtgaacggtt cttccggtat cgccgtaggt
     1441 atggcaacca acatcccgcc gcacaacctg acggaagtca tcaacggttg tctggcgtat
     1501 atcgatgatg aagacatcag cattgaaggg ctgatggaac acatcccggg gccggacttc
     1561 ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag aagcttaccg taccggtcgc
     1621 ggcaaggtgt atatccgcgc tcgcgcagaa gtggaagttg acgccaaaac cggtcgtgaa
     1681 accattatcg tccacgaaat tccgtatcag gtaaacaaag cgcgcctgat cgagaagatt
     1741 gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg cgctgcgtga cgagtctgac
     1801 aaagacggta tgcgcatcgt gattgaagtg aaacgcgatg cggtcggtga agttgtgctc
     1861 aacaacctct actcccagac ccagttgcag gtttctttcg gtatcaacat ggtggcattg
     1921 caccatggtc agccgaagat catgaacctg aaagacatca tcgcggcgtt tgttcgtcac
     1981 cgccgtgaag tggtgacccg tcgtactatt ttcgaactgc gtaaagctcg cgatcgtgct
     2041 catatccttg aagcattagc cgtggcgctg gcgaacatcg acccgatcat cgaactgatc
     2101 cgtcatgcgc cgacgcctgc agaagcgaaa actgcgctgg ttgctaatcc gtggcagctg
     2161 ggcaacgttg ccgcgatgct cgaacgtgct ggcgacgatg ctgcgcgtcc ggaatggctg
     2221 gagccagagt tcggcgtgcg tgatggtctg tactacctga ccgaacagca agctcaggcg
     2281 attctggatc tgcgtttgca gaaactgacc ggccttgagc acgaaaaact gctcgacgaa
     2341 tacaaagagc tgctggatca gatcgcggaa ctgttgcgta ttcttggtag cgccgatcgt
     2401 ttgatggaag tgatccgtga agagctggag ctggttcgtg aacagttcgg tgacaaacgt
     2461 cgtactgaaa tcaccgccaa cagcgcagac atcaacctgg aagatctgat cactcaggaa
     2521 gatgtggtag tgacgctctc tcaccagggc tacgttaagt atcagccgct ttctgaatac
     2581 gaagcgcagc gtcgtggcgg gaaaggtaaa tctgccgcac gtattaaaga agaagacttt
     2641 atcgaccgac tgctggtggc gaacactcac gaccatattc tgtgcttctc cagccgtggt
     2701 cgcgtctatt cgatgaaagt ttatcagttg ccggaagcca ctcgtggcgc gcgcggtcgt
     2761 ccgatcgtca acctgctgcc gctggagcag gacgaacgta tcactgcgat cctgccagtg
     2821 accgagtttg aagaaggcgt gaaagtcttc atggcgaccg ctaacggtac cgtgaagaaa
     2881 actgtcctca ccgagttcaa ccgtctgcgt accgccggta aagtggcgat caaactggtt
     2941 gacggcgatg agctgatcgg cgttgacctg accagcggcg aagacgaagt aatgctgttc
     3001 tccgctgaag gtaaagtggt gcgctttaaa gagtcttctg tccgtgcgat gggctgcaac
     3061 accaccggtg ttcgcggtat tcgcttaggt gaaggcgata aagtcgtctc tctgatcgtg
     3121 cctcgtggcg atggcgcaat cctcaccgca acgcaaaacg gttacggtaa acgtaccgca
     3181 gtggcggaat acccaaccaa gtcgcgtgcg acgaaagggg ttatctccat taaggttact
     3241 gaacgtaatg gtttagtggt tggcgcggta caggtagatg actgcgacca gatcatgatg
     3301 atcaccgatg ccggtacgct ggtacgtact cgcgtttcgg aaatcagcat cgtaggccgt
     3361 aacacccagg gcgtgatcct catccgtact gcggaagatg aaaacgtagt gggtctgcaa
     3421 cgtgttgctg aaccggttga cgaggaagat ctggatacca tcgatggcag tgccgcggaa
     3481 ggggacgatg aaatcgctcc ggaagtggac gttgacgacg agccagaaga agaataattt
     3541 tacttcttca tgccaaaagg gagctatctc ccttgtttga attgaaaagt ccaggctgca
     3601 aagtctgggc ttttgtcgta ttagggcacg gtaaagtttg gctgtgcccg taaaaaatgg
     3661 ctggctatac acaaggaatg tggcaatgag tggtgaaaaa aaggcgaaag gctggcggtt
     3721 ctatggtctt gtaggttttg gcgcaatagc actgctttcc gctggcgtct gggcgttgca
     3781 atatgctggc agtgggccag aaaaaacgtt gtcgccgctg gtggtgcaca acaatctgca
     3841 aatcgatctc aatgagccgg acctctttct cgacagcgac tctctgagcc agcttcccaa
     3901 agatctcctc accattccgt ttctccacga tgttctgagc gaagatttcg ttttctatta
     3961 tcagaatcat gccgatcgtc tgggcattga aggcagcatt cgtcgtattg tctatgaaca
     4021 cgatctcacg ctgaaagata agctcttttc gtcactctta gatcagcccg cgcaggcagc
     4081 gctgtggcac gataaacaag gccatctttc acattatatg gtgctgatcc agcgcagtgg
     4141 tttaagcaaa ctgctggagc cattgttgtt tgccgctacc agcgacagcc agttaagcaa
     4201 aacggaaatc agtagcatca agataaatag tgaaactgtt cctgtttatc agttgcgcta
     4261 taacggcaat aacgccctga tgttcgcgac ttatcaggac aagatgctgg tgttttccag
     4321 cacggatatg ttgtttaaag atgatcagca ggataccgaa gccacggcga tcgcaggtga
     4381 tttgttgagc ggcaaaaaac gctggcaagc aagctttggc ctggaagagc gtgctgctga
     4441 aaaaacgcca gtacgccagc gcatcgtggt cagcgccagg ttgctgggtt ttggctacca
     4501 gcggttaatg ccttcttttg ctggcgtacg cttcgaaatg ggtaacgacg gctggcacag
     4561 ttttgtggcg ttaaatgatg aatccgccag cgtagatacc agtttcgatt ttacgccggt
     4621 ctggaacagt atgcctgccg gggccagctt ctgtgtggtg gtgccgtatt cacacggtat
     4681 tgccgaagag atgctttcgc acatcagtca ggaaaacgac aagttgaatg gggcgttaga
     4741 cggtgccgcg gggctgtgct ggtatgcaga ctcaaaattg caaaccccgc tgtttgtcgg
     4801 tcagtttgat ggcactgccg aacaggcgca attgccaggg aaactgttta cgcaaaatat
     4861 tggtgcgcac gaaagcaaag cgccagaagg tgttttgccg ataagccaga ctcagcaggg
     4921 cgaagcgcaa atctggcgtc gcgaagtgag ttcccgatac ggtcagtatc cgaaagcgca
     4981 ggcggcgcaa cccgatcaat taatgtcgga ttattttttc cgcgtgtcgc tggcgatgca
     5041 aaacaaaacg ctgcttttct ccctcgatga cacgctggtt aataacgccc tgcaaacact
     5101 gaataaaaac cgcccggcaa tggtggatgt aatacccact gatggcatcg ttccgctcta
     5161 tatcaatcca caaggcgtgg cgaaactgct gcgtaacgaa acgttgacca gtctgccgaa
     5221 gaatctcgaa ccggtttttt ataacgccgc acaaacttta ttaatgccga agctggacgc
     5281 tttatctcaa caaccgcgtt atgtcatgaa gctggcccag atggaacccg gtgccgcctg
     5341 gcagtggcta cccataacct ggcagccgct atgaggcacg ggctgctggc gctgatttgc
     5401 tggctgtgtt gtgttgttgc ccatagcgaa atgctgaatg tcgaacaatc cgggctgttt
     5461 cgcgcctggt ttgtacgcat tgcacaagaa cagctccgcc aggggccaag tccacgctgg
     5521 tatcagcagg attgtgcggg cctggtgcga tttgcggcga acgagacgct gaaagttcac
     5581 gatagtaaat ggctaaaaag taacggtttc tctagccagt atttgccgcc agagatgacg
     5641 ctaacacctg gacagcgtca actggcgcaa aactggaatc aggggaacgg gaaaaccggc
     5701 ccctacgtga ccgcgattaa tttgattcag tacaacagcc agtttattgg ccaggacata
     5761 aaccaggcgc tgcctggcga tatgattttt ttcgatcagg gcgatgccca gcacttaatg
     5821 gtctggatgg ggcgttacgt catctaccac accggaagcg ccacgaaaac tgacaacgga
     5881 atgcgcgcag tcagtctgca acaacttatg acatggaagg acacccgatg gatacccaac
     5941 gattccaatc ccaatttcat tggcatttat cgtttaaatt ttctggcgcg atagcagcgt
     6001 gcttgtccct gtctcttgtg ggaacagggc ttgctaatgc tgatgattcg cttccttcca
     6061 gtaactatgc gccgcccgcc gggggaacat tctttttgct tgctgacagc agttttagca
     6121 gcagtgaaga ggcgaaagtg cgactggaag cgccggggcg tgattatcgg cgctatcaga
     6181 tggaagagta cggcggcgtg gacgttcgcc tgtatcgtat tcctgacccg atggcatttt
     6241 tgcgccagca gaaaaacctg catcgcattg tggtgcaacc gcaatatctg ggcgacgggc
     6301 tgaacaatac gctgacctgg ctgtgggata actggtacgg caaatctcgc cgcgtaatgc
     6361 agcgtacttt ctcttctcag tcacggcaga atgtgactca ggcattaccc gaattacatc
     6421 tcggcaatgc cattattaaa ccttcccgtt atgtacagaa caaccagttt tccccgctga
     6481 aaaaatatcc cttggtggaa cagttccgtt atccactatg gcaggctaaa ccggtcgagc
     6541 cgcagcaagg ggtaaaactg gaaggcgcat ccagcaattt catctcgccg cagccgggta
     6601 acatttatat tcctctcggc caacaagagc cgggactgta cctcgtcgag gcgatggttg
     6661 gtgggtatcg ggcgacgacg gtggtgtttg tttccgatac cgtggcgctt agcaaagtgt
     6721 caggcaacga gcttctggtg tggaccgcgg gtaaaaaaca gggtgaagcg aagcccggct
     6781 cagagatctt gtggactgac ggtcttggcg tgatgacccg cggtgtgacc gatgacagcg
     6841 gtaccttgca gttacaacat atatcgccag aacgttcata cattctgggt aaggatgctg
     6901 aaggcggtgt ttttgtctcc gagaacttct tctacgaaag cgaaatctac aacacccgct
     6961 tgtatatttt taccgatcgc ccgctatatc gcgcaggcga tcgtgtcgat gttaaagtga
     7021 tgggccgcga gttccacgat ccgttgcatt catcccccat cgtcagcgcc ccggcgaagc
     7081 tttcggtgct ggacgctaac ggcagtctgt tgcaaaccgt cgatgtcacg ctggatgcgc
     7141 gcaatggcgg gcagggaagt ttccgcctgc cagaaaatgc cgttgccgga ggttatgagt
     7201 tacgtcttgc ttaccgcaat caggtctata gcagcagttt tcgcgtggca aactacatca
     7261 agccacattt cgagattggt ttagctctcg acaaaaaaga gttcaaaact ggcgaagcgg
     7321 tcagcggcaa actgcaactg ctctacccgg atggcgagcc ggtaaaaaat gcccgcgtgc
     7381 agttaagttt gcgcgctcag caattatcaa tggtcggtaa cgatttgcgt tatgccggac
     7441 gtttccccgt gtcgctggaa ggcagcgaaa cggtgtccga cgccagcggt catgtggcgt
     7501 taaatctccc cgccgccgat aaaccgagcc gttatttgtt aaccgtctcc gccagtgacg
     7561 gcgcggcgta tcgtgtcacc accaccaaag agatcctcat tgaacgcggt ctggcgcatt
     7621 actcattaag tactgccgca caatacagta atagcggcga gtcggttgtg ttccgttatg
     7681 ccgcgctgga atcttcaaaa caggttcctg ttacgtatga atggttgcgt ctcgaagacc
     7741 gcacgagcca tagcggagag ctaccgtcag gcggcaaatc ctttaccgtc aatttcgcta
     7801 aacctggcaa ctacaatctg acattacgcg ataaagacgg cttaattctc gctgggttaa
     7861 gtcatgccgt cagcggtaag ggcagcacgg cgcatactgg tacggtagat atcgtggcgg
     7921 ataaaacgct gtaccagcca ggcgaaactg cgaagatgct gattaccttt ccggagccaa
     7981 ttgatgaagc attattgacg ctggaacgcg atcgcgtgga acagcagtcg ctgctttcgc
     8041 atccggcaaa ctggctgacg ttacaacgtt taaacgatac ccagtatgaa gcccgggttc
     8101 cagtgagcaa ttcctttgcg cctaacatca ctttttcggt gctgtatacc cgtaatggtc
     8161 agtacagttt tcagaacgcc gggatcaaag ttgccgttcc tcagcttgat atccgggtga
     8221 aaacggacaa aacccattac cagcctggtg aactggtcaa tgtcgaatta acctcgtcgc
     8281 tgaaaggtaa acctgtttct gcgcagctaa cggtaggcgt ggtcgatgaa atgatctacg
     8341 cgctgcaacc agaaatcgcg ccgaatatcg gcaaattttt ctatccgctg gggcgtaaca
     8401 atgtgcgtac cagctccagt ctgtcgttta tcagctacga ccaggcgctc tccagcgagc
     8461 cggttgcgcc tggcgcaact aaccgcagcg agcggcgagt aaaaatgctt gaacgtccac
     8521 ggcgtgaaga ggtggatacc gcggcatgga tgccgtcact cacaaccgat aaacaaggca
     8581 aagcgtattt cacgttcctg atgcctgatt cgttaacccg ctggcgtatc accgcgcgtg
     8641 ggatgaacgg cgacgggctg gtcgggcagg ggcgtgctta tctgcgttcg gaaaaaaatc
     8701 tctacatgaa gtggagtatg ccaacggtgt atcgcgtggg cgacaaaccg gcggcaggac
     8761 tgtttatctt cagtcagcag gataacgaac cggtggcgct ggtgactaaa tttgcaggcg
     8821 ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg ctggcgcaga
     8881 acattcagca atctggcttg ttaagcgcag aactgcaaca aaatgggcaa gtgcaggaca
     8941 gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacag cagaaaaatg
     9001 tcatgctcgg cggtggcgag aacgcgctga tgttgcccga gcaggctagc aatatccggc
     9061 tacaaagtag cgaaacgccg caggagattt tccgcaacaa tcttgatgcg ttagtcgatg
     9121 gaccatgggg tggcgtgatc aacaccggta gccgtctgat cccgctcagt ctcgcctggc
     9181 gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg attcaggata
     9241 accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg tggggtgaag
     9301 atggcaatgg tgacgccttc cttacggcat gggcatggta cgccgactgg caggccagcc
     9361 aggcgctcgg cgtaacgcaa caaccggaat actggcagca tatgctcgac agttatgccg
     9421 agcaggcgga taacatgccg ttattgcatc gggcgctggt gctggcgtgg gcacaggaga
     9481 tgaatctgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc cggcgcggaa
     9541 ctaaaaatga agatttctct gaggaagaca tccgcgatat caatgacagc ctgatcctcg
     9601 atacaccgga atctccactg gcagatgcgg tggcaaacgt cttaaccatg acgttgctga
     9661 aaaaagcgca gttgaagtcc acggtgatgc cacaggttca gcaatatgcg tgggataaag
     9721 cggcaaacag caatcagccg ctggcgcaca cggttgtgct gctcaatagc ggtggtgacg
     9781 ctacccagac ggccgctatt ttaagtggtt tgaccgctga gcaatccact attgagcgcg
     9841 cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccagtt gttttgcctg
     9901 cgcctgcggg cgcatgggct aaacataagt taactggagg gggcgaagac tggcgttggg
     9961 ttggtcaggg tgtgccggac attctctctt ttggtgacga attatcgccg caaaatgtgc
    10021 aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg
    10081 aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact ctgcagccgg
    10141 tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg cttaccagcg
    10201 agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg ggagccgacg
    10261 ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa cagcaggggc
    10321 aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag
    10381 aactgacggg aacggtcact ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg
    10441 ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt gttgcggcag
    10501 ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc gtctggctat
    10561 tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg gtgctgcgcg
    10621 gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa gtctcagcat
    10681 tacctgactc gctcaccacg ccgttaggga gtttgtggaa actgtatgtt tacgcctggc
    10741 tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc ccggaagagg
    10801 tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta cgttcctgtg
    10861 ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg caatactggc
    10921 aacagcgtca ggcacctgcg tggttagcgt ctcttacaac gctgaagcca gaaacctcgg
    10981 taacggttaa atcgctgctc gattcattag cgacgttacc tgcgcaaaac aaagctcagg
    11041 aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc atgctcggta
    11101 gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa atccgccagg
    11161 gtggttttgc tggctggtta accgatggta caccgctgtg ggttactggc agtggcacca
    11221 gcaaaacggt actaacccgt tatgcgacgg tgcttaatcg ggttttgcca gtgccgacgc
    11281 aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg ctgaagaaaa
    11341 tcacagcgga aaagagtacg acggcagtaa aacccggcgt acttaatggg cgctatcgtg
    11401 tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg accttactga
    11461 gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac gttgccagag
    11521 ttctggatcg cgaggcgaaa agtacgcccc ctgaggcagc gaaagccatg accgtggcga
    11581 ttcgcacttt tttacagcaa aacgccaatc gggaaggcga ttgcctgacc atccccgaca
    11641 gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga acgatgaccg
    11701 cctggacgca ggatctcatc tatgcaggcg atccggttca ttatcatggc agtcgcgcta
    11761 ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcagggg gagcgttacg
    11821 accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga gctccacgct
    11881 caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc
    11941 ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg gtctgtcgtt
    12001 tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt cgtaacttct
    12061 ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacctggcc tttgatggtt
    12121 atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta ttaatggact
    12181 aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc tgtcgctcac
    12241 agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca tccggcagag
    12301 ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt caacatggca
    12361 gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc tgcggcgggt
    12421 aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag agttgaatcc
    12481 gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt gcaggtcatc
    12541 agcccggatg ggcaaagccg acaaaaaatg cagttttact caacgccggg tacaggaacg
    12601 attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga tctcgatctc
    12661 catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct gaaaaacagt
    12721 ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc catgccagca
    12781 ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag cgaaacggaa
    12841 ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga gaaacaggaa
    12901 acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag ttttgactgg
    12961 tgatccatcc cttcacacaa cctggctctc tcttttttca gataagactg ctgggcgatc
    13021 tgccgacgcc aggatataac cattaacaag cgtcggcaga ttggtcattt cttatttttc
    13081 gtactgagaa ctgtccatct actccggctt tcctggttaa ggcaatggtt tctaagcgat
    13141 acgcattatt gcaatggtga cagaacattg cggtaacacg cttttaccgc taccttaacc
    13201 acactccatc ggtcacctga ggcggagctt cgcccctttg aaataccttg cttcttttcg
    13261 tacaaccctg aaagcctcgc gctacatgtt cagagcattg gcgttagtgc tctggctgtt
    13321 gattgctttt tcatccgttt tttacatcgt taatgcgtta catcagcgag aatcggaaat
    13381 tcgtcaggaa tttaatctga gttccgatca ggctcagcgc tttattcaac gcacctctga
    13441 tgtgatgaaa gagctgaagt acatcgccga aaatcgctta tcggcagaaa acggtgtgct
    13501 ttccccgcgt ggacgagaaa cgcaggcgga tgtgcctgcg tttgaaccgc tgtttgccga
    13561 ctccgattgt tccgcaatga gtaacacctg gcgaggttct ctggagtcat tggcgtggtt
    13621 tatgcgctac tggcgcgata atttttctgc ggcttacgat ctcaaccggg tatttttaat
    13681 cggcagtgat aacctctgca tggccaattt cggtctgcgt gatatgccag tggaacgcga
    13741 taccgcgttg aaagctttgc atgaacgcat caataaatat cgaaatgcac cacaagatga
    13801 tagcggcagt aacctctact ggatcagcga aggtccgcgc cctggcgtcg ggtattttta
    13861 cgcgttgacg ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg tcgagcagac
    13921 catccggatg gagaactttt tcttaccggg tacgttgccg atgggggtta ccattcttga
    13981 tgaaaatggt cataccctga tttcgcttac cggaccagaa agtaaaatta agggcgatcc
    14041 tcgctggatg caggaacgct cctggtttgg ctatacggaa gggttccggg agctggtgct
    14101 gaagaaaaat ctgccaccct catcgctaag catcgtgtat tcggtgccgg ttgataaggt
    14161 gctggaacgc attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc ttgccggagc
    14221 tgcattgttt actctcgcgc ggatgtacga gcgacgtatt ttcattccgg cggaaagcga
    14281 cgccctgcga ctggaagaac atgagcagtt caatcgcaag attgtcgcct ccgcgccagt
    14341 gggtatctgt attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg aactggcgca
    14401 tacctatctc aatatgctta cgcatgagga ccgccaacga ctgacgcaaa ttatctgtgg
    14461 gcagcaggtc aattttgttg atgtcctgac cagcaacaat accaatctgc aaatcagctt
    14521 cgtccattcg cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg atgtttcttc
    14581 gcgcgtgaag atggaagagt cgttgcagga gatggcacaa gcagcggaac aggcgagcca
    14641 gtcaaaatcg atgttccttg ccaccgtcag tcatgagctg cgaacgccgc tgtatggcat
    14701 tatcggtaac ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg atcggctggt
    14761 gacggcaatg aacaactctt ccagcctgtt gttgaaaatt atcagcgata ttctcgattt
    14821 ctcgaagatt gaatcggaac agttgaagat cgaaccgcgt gagttttcac cgcgtgaagt
    14881 gatgaaccac atcaccgcca actatttacc gctggtggta cgcaagcagt taggcttgta
    14941 ctgctttatt gaaccggatg tgccagtggc cttaaatggc gacccgatgc gtttacagca
    15001 ggtcatctcc aacctgttga gtaacgccat aaaattcacc gataccggct gtatagtttt
    15061 gcatgtccgc gctgatggcg attatctctc tatccgtgtt cgcgataccg gcgtggggat
    15121 tccggcgaaa gaagtggtgc gcttgtttga tcccttcttc caggtcggaa cgggcgtaca
    15181 gcgaaacttc caggggaccg gtctgggtct ggcgatttgt gaaaaactga tcagcatgat
    15241 ggacggcgat atctcggtag attcagaacc gggaatgggc agccagttta ccgtgcgtat
    15301 tccgttgtac ggcgctcagt acccgcagaa aaaaggcgtg gaagggttga gtggtaaacg
    15361 ctgctggctg gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca gtttgcagcg
    15421 cagcggcatc gtcgttacaa catacgaagg gcaggaaccg actcccgaag atgtgttaat
    15481 tactgacgag gtagtgagta aaaaatggca gggtagagcg gtagtgacct tctgtcgtcg
    15541 ccatattggt attccgctgg agaaagcgcc aggggagtgg gtacacagtg tggctgcccc
    15601 gcatgagcta ccggcattgt tggcgcgtat ttatttgatc gagatggaga gcgacgatcc
    15661 tgctaacgct ctgccgtcga cggacaaagc ggtcagcgat aatgacgata tgatgattct
    15721 ggtcgtggat gatcatccga ttaaccgacg tctgctggca gatcagttgg gatcgttggg
    15781 ctatcaatgt aaaaccgcga atgatggtgt cgatgcgctt aatgtactta gcaagaatca
    15841 tattgatatc gtgcttagcg acgtcaacat gccaaatatg gatggttacc gcttgacgca
    15901 acgcattcgt cagttgggac tgacgttgcc ggtaatcgga gtaactgcta atgcgttggc
    15961 tgaagagaag cagcggtgtc tggagtccgg tatggacagc tgcctgtcga agccggtaac
    16021 gctggatgtg ataaaacaga cgctgacggt atatgccgag agggtcagga aatcgcggga
    16081 atcgtaggac ggataaggtg tttatgccgc atctggcatt cagtgcaaat gccagatgcg
    16141 acgctgacgc gtcttatctg gcctacttta atgtaatctg ttgaaataat gggaatcgta
    16201 ggccggataa ggcgtttacg ctgcatccgg caatcggtgc aaatgccaga taagacacta
    16261 acgcgtctta tctggcctac agatgattag tctttatctg ccggacttaa ggtcacagaa
    16321 gagagataat tcagcagggc gatatcgttc tcgacaccca gcttcatcat cgcagatttc
    16381 ttctggctac tgatggtttt aatactgcgg ttcagctttt tagcgatctc ggtcaccagg
    16441 aagccttccg caaacaggcg cagaacttca ctctcttttg gcgagagacg cttgtcaccg
    16501 taaccaccag cactgatttt ttccaacagg cgagaaacgc tttccggggt aaatttcttc
    16561 cctttctgca gcgcggcgag agctttcggc agatcggtcg gtgcaccttg tttcagcacg
    16621 atcccttcga tatccagatc caataccgca ctaagaatcg ccgggttgtt gttcatggtc
    16681 agaacaatga tcgacaggct tgggaaatgg cgcttgatgt acttgattaa ggtaatgcca
    16741 tcgccgtact tatcgccagg catggagaga tcggtaatca acacatgcgc atccagtttc
    16801 ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt cacccactca
    16861 atttgctcaa gtgatttgcg aataccgaac aagactatcg gatggtcatc ggcaataatt
    16921 acgttcatat tgttcatgta ataggctacc ttgctacagc aagctcttga cataactgtc
    16981 aatgtcgctg atgtattttt ctattcctgg aacatccttc tcacgaatca gatgttccag
    17041 cgtttcacat aactgcttgc cgggtaccag atttagcatg gcaaatacgc ctttaagacg
    17101 atgagccgtt tgggctaacg cagcaaagtc actggttgct gcttcagtat acagcctctt
    17161 aacatcatcc ggtactgtgt ctacaaagag cgcataatag ccgctggcat ggagttgcgc
    17221 attttcatct ccgcccagag gcgattctgt cacctcttcc tgcgccagtt gcacttcaat
    17281 taattgtaag accgcttcct gcatagcgtt gctcatattg aagttgacgc acaattgacc
    17341 aggcccaatt tcccgtacgc cagactcatc atcgcttaaa agcaagccag aggcagtaag
    17401 attagacgga ttatccgtta aaaagatatc ataatcttga ctaattaatc tttcatcggg
    17461 tgtgatacag gttgcacccc aattttctaa ctggcgagtg acaatattcc gaatttctgc
    17521 cgaagtaaca tccaccatta cgcagacatc atccagtaaa cgctcttctt cctcttcaac
    17581 ttccgggtca gctgcgagca ttttgatatg cacagagtag cgtgtaccaa gcccatcccg
    17641 cgttttgatg tttaaatgac cgcccagttt acgtgccagt tgatcgctca gccagaatgc
    17701 cagcgggtcc gccttgccat agcgatcgtt ttgggtctgg ttgataaacg ggaagtgcaa
    17761 attatccatt tcatgaatac ttacgccttc tcccgtgtcc agaatgcgga acgtcaggcg
    17821 gtcttcggag gactcatcct gatcaacctc aagggtgatt tttcccaatt gcgttgaggt
    17881 cacggcatat tgcatcagta gcagcaaaat acgtcgtaag gcatcgcgat cgccgcggcg
    17941 catatcgtgt gctttcagat gattgttaat cagcagttgc agacctttac gcttgatggc
    18001 aggcaacact gaaggcacaa cttcatcaat taaatcctgc acggagaaca gcaccgtctc
    18061 acttttccag ctatcatccg caagcatgtt cgctaactgt atttcatcaa ccagccgcac
    18121 cagcacatct gcctgattcg ccagttgttt gctttccggg gcgttgagtt tagccgcgct
    18181 ctccgccagg gactgtgcgg gttctttcag cgcatcgcca atgtttttca taaaggtcat
    18241 ccgcccctgc tggtttttct catacagacg ctgcgcctgc ttgagtttct tgtttaccag
    18301 cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg cgcggcgcga cctggctgcg
    18361 gaacatgcgg atctcataca gctcgttatt gatcgtcgcc tgaataatcc cctgatgctg
    18421 ttccgccatg gtggtgatgt tttgcagatt caaatgcggc agcaaatgat cggcaatttt
    18481 gttacttatg acagtgcggt tcgattcctg atcgtgaacc agcaggccga gcggcagcag
    18541 tgagactatc tcttcattaa ttgcccgtaa aatgcgcaat tcgttattga ccgccgtgct
    18601 gggtagactt tctgtactgc ggctggagaa atggcggaat gtggtatagc caaataacgc
    18661 cagcgccagc aaaccgatgt tcagcagcag tggcagcaga atgttttgca acgtatccag
    18721 caataaggtg ccataaggaa cctgccagac caggcgcata tcggtagagt tgagtgccga
    18781 ggagatttca atcttcgtac tgttaaagtg gatactgacg ctatccgtcc cttctttctc
    18841 attatcattg tctcccgttg ccgtcgcgtc tggctcaagg cggaaactgt ccagcggcat
    18901 acccggtggg atcaaatcat taatcggcag atcaaaagcc acgaccgttg ccagatgtcc
    18961 cggctggttg aacgtggtac gcaaggtaaa gtaatgaccg ttctgccagg ccaggcggcg
    19021 catgttagaa aaactttcgc gttcatcgag ggcgttggcc tgttgcaaca tttctgcacg
    19081 acgtgaatca acaatgtcac tgacggtcga ttctttaaat ccggaggtga gatctttgag
    19141 gggcagggtt gagatcagca ccagactgtt atcctgacca ttcaggtaat acatcgacca
    19201 cggtacattt tctgcgcccc acaatgtgtc cagataggtg gacatccgtt gagtcatctc
    19261 aagcgttgag ttgtcgtgag agccaaagat taacgcttcc gttttgcggc gcggtttttc
    19321 cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa ccttcgccgg aggaggggga
    19381 ggtcgtcgcg gcaatgttgt cgtagatctg ccaggtcacg taacgccagg tatcgacgcg
    19441 cttttggata gcgtgggtaa tgtcgacaat ctggtaactt ttatctttca gccaggcgtt
    19501 aacggcgctt tgtaccatta cccccatcgt caccagtaac acaatgatca acagtaaaaa
    19561 gaagcgggta atgctccccg gtaggagtga aaagcgggtc gtggccgttg tctctttctg
    19621 acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg atagggcagt ataaagggta
    19681 cagtgtgaaa ttccagactc ttacctgatt gatgattctc tattctcccg tgatatttat
    19741 catcctgcat tgggggaaat agatgtacaa gcgccatttt gctgtacaaa taacctacaa
    19801 aaagcccaac aaaaataatt gcaaataaac aaagattgct ggaaattatg ctgatgttaa
    19861 ttatttgtga aatagttaac aagcgttata gtttttctgt ggtagcacag aataatgaaa
    19921 agtgtgtaaa gaagggtaaa aaaaacggat gcgaggcatc cggttgaaat aggggtaaac
    19981 agacattcag aaatgaatga cggtaataaa taaagttaat gatgatagcg ggagttattc
    20041 tagttgcgag tgaaggtttt gttttgacat tcagtgctgt caaatactta agaataagtt
    20101 attgatttta accttgaatt attattgctt gatgttacgt gcttatttcg ccattccgca
    20161 ataatcttaa aaagttccct tgcatttaca ttttgaaaca tctatagcga taaatgaaac
    20221 atcttaaaag ttttagtatc atattcgtgt tggattattc tgcatttttg gggagaatgg
    20281 acttgccgac tgattaatga gggttaatca gtatgcagtg gcataaaaaa gcaaataaag
    20341 gcatataaca gagggttaat aacatgaaag ttaaagtact gtccctcctg gtcccagctc
    20401 tgctggtagc aggcgcagca aacgctgctg aagtttacaa caaagacggc aacaaattag
    20461 atctgtacgg taaagtagac ggcctgcact atttctctga caacaaagat gtagatggcg
    20521 accagaccta catgcgtctt ggcttcaaag gtgaaactca ggttactgac cagctgaccg
    20581 gttacggcca gtgggaatat cagattcagg gcaacagcgc tgaaaacgaa aacaactcct
    20641 ggacccgtgt ggcattcgca ggtctgaaat tccaggatgt aggttctttc gactacggtc
    20701 gtaactacgg cgttgtttac gacgtaactt cctggaccga cgtactgcca gaattcggtg
    20761 gtgacaccta cggttctgac aacttcatgc agcagcgtgg taacggcttc gcgacctacc
    20821 gtaacactga cttcttcggt ctggttgacg gcctgaactt tgctgttcag taccagggta
    20881 aaaacggtag cgtaagcggc gaaggcatga ccaacaacgg tcgtggtgct ctgcgtcaga
    20941 acggcgacgg cgttggcgga tctatcactt atgattacga aggcttcggt atcggtggtg
    21001 cgatctccag ctccaaacgt actgatgatc agaacagccc gctgtacatc ggtaacggcg
    21061 accgtgctga aacctacacc ggtggtctga aatacgacgc taacaacatc tacctggctg
    21121 ctcagtacac ccagacctat aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag
    21181 cacagaactt tgaagctgtt gctcagtacc agttcgactt cggtctgcgt ccgtctgtag
    21241 catacctgca gtctaaaggt aaaaacctgg gtaccatcgc tggtcgtaac tacgacgacg
    21301 aagatatcct gaaatatgtt gatgttggtg ctacctacta cttcaacaaa aacatgtcca
    21361 cctacgttga ctacaaaatc aacctgctgg acgacaacca gttcactcgt gacgctggca
    21421 tcaacactga taacatcgta gctctgggtc tggtttacca gttctaatct cgattgatat
    21481 cgaacaaagg gcctgcgggc ccttttttca ttgttttcag cgtacaaact catttttttg
    21541 gtgtactctt gcgaccgttc gcatgaggat aatcacgtat ggaaataagc tttacccgcg
    21601 tggcactgct ggctgccgcg ctcttctttg ttggttgcga tcaaaaacca caacccgcca
    21661 aaacccacgc tactgaagtt accgttcttg aaggcaaaac catgggtacc ttctggcgtg
    21721 ccagcatccc gggcattgat gccaaacgca gtgccgaact taaagaaaag attcagaccc
    21781 agctggacgc cgacgatcag ctgctttcga cctataaaaa agattccacg ctgatgcgct
    21841 ttaacgactc gcaaagtttg tcgccgtggc cggtaagtga agcgatggcc gatatcgtca
    21901 ccacctcgct gcgcattggc gcaaggaccg atggtgcgat ggatataacc gtcgggccgc
    21961 tggtgaatct gtggggcttt ggcccggaac aacagccggt tcaaattccg agccaggaac
    22021 agatcgatgc gatgaaagcc aaaaccggct tacagcacct gacggtcatc aatcagtcgc
    22081 atcagcaata tctgcaaaaa gacctgccgg atttatatgt cgatctctcc accgtcggtg
    22141 aaggttatgc ggcggatcac ctggcacgct tgatggagca ggaaggtatt tcacgttatc
    22201 tggtgtcagt tggtggtgct ctggatagcc gtggtatgaa cggtgaaggc cagccgtggc
    22261 gggtagcgat tcaaaaacca accgataaag aaaacgcggt tcaggccgtg gtggatatca
    22321 acggtcatgg cattagcacc tctggcagct accgtaacta ttacgaactg gacggcaaac
    22381 gtctttctca tgttatcgat ccgcaaaccg ggcgtcctat cgaacacaat ctggtatccg
    22441 tgacggtgat tgccccgacg gcgctggaag ccgatgcctg ggatactggc ttaatggtac
    22501 tcgggccgga gaaagccaaa gaggttgttc gccgggaagg gctggcggtc tatatgatca
    22561 ccaaagaagg tgatagcttt aaaacctgga tgtcgccgca gtttaaaagc ttccttatca
    22621 gcgaaaaaaa ttaaagcgca agattgttgg tttttgcgtg atggtgaccg ggcagactaa
    22681 aggctatcct taaccaggga gctgattatg aaaaaagcca catgcttaac tgacgatcaa
    22741 tgctggcaat ctgtcttagc ccgcgacccg aatgccgacg gcgaattcgt tttcgccgtg
    22801 cgtaccacag gcatcttttg ccgtccgtct tgccgcgcca gacatgcttt gcgggaaaac
    22861 gtctccttct acgcaaatgc cagcgaggca ctcgccgctg gctttcgccc ctgcaaacgt
    22921 tgtcagccag aaaaagccaa tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt
    22981 cgactgctgg aacaggaaac gcctgtaacg ctggaagcct tagccgacca ggtggtgatg
    23041 agtccatttc atctacatcg gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg
    23101 caacaggcct ggcgcgctcg ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg
    23161 acgtctattc ttaacgccgg attccccgac agcagcagtt actaccgcaa agccgatgaa
    23221 acgctgggca tgacggctaa acaattccgt cacggtggcg aaaatctggc ggtgcgttac
    23281 gcgctggctg attgtgagct gggtcgtagc ctggtggcag aaagcgagcg ggggatttgc
    23341 gcgatattgc tgggcgatga tgacgcgaca ctaatcagcg agttgcagca gatgtttccc
    23401 gctgccgaca acgcgcctgc cgatctgatg tttcagcaac atgtgcgtga agtgatcgcc
    23461 agcctcaatc aacgcgatac gccgctgacg ttaccgctgg acattcgcgg cactgctttt
    23521 cagcaacaag tctggcaggc actgcgcacg ataccttgcg gtgaaaccgt cagttatcag
    23581 caactggcta acgccatcgg caaaccgaaa gcggtacggg ccgttgccag cgcctgtgcc
    23641 gccaacaagc tggctatcat aataccctgt catcgggtgg tccgtggtga tggcacactt
    23701 tccggttacc gctggggcgt gtcgcgtaaa gcgcaactgc tgcgccgcga agctgaaaat
    23761 gaggagaggt aatgttggat ctgtttgccg atgctgaacc gtggcaagag ccactggcgg
    23821 ctggtgcggt aattttacgg cgttttgctt ttaatgctgc ggagcaacta atccgcgata
    23881 ttaatgacgt tgccagccag tcgccgtttc gccagatggt cacccccggg ggatatacca
    23941 tgtcggtggc gatgaccaac tgtgggcatc tgggctggac gagccatcgg caaggttatc
    24001 tctattcgcc cattgatccg caaacaaata aaccgtggcc cgccatgcca cagagttttc
    24061 ataatttatg tcaacgtgcg gctacggcgg cgggctatcc agatttccag ccagatgctt
    24121 gtcttatcaa ccgctacgct cctggcgcga aactgtcgct gcatcaggat aaagacgaac
    24181 cggatctgcg cgcgccaatt gtttctgttt ctctgggctt acctgcgatt tttcaatttg
    24241 gtggcctgaa acgtaatgat ccgctcaaac gtttgttgtt ggaacatggc gatgtggtgg
    24301 tgtggggcgg tgagtcgcgg ctgttttatc acggtattca accgttgaaa gcggggtttc
    24361 atccactcac caccgactgc cgctacaacc tgacattccg tcaggcaggt aaaaaagaat
    24421 aaaaataaga attattattg ctgtgcgcgc gaagatgttt aaactgcggg ctgtaattca
    24481 ttgtccgggt tttctgcatg gaacttcttg tacttgtctg gcggcagtat cgctggccat
    24541 ttatcagtgt gatggcgcta agcctcgcca gtgcggcatt aggcattggc ttaattgctt
    24601 ttatcaatca gcgccttatc gaaacggcgg ataccagtct gctggtattg ccggagtttc
    24661 tgggattatt gctgctgttg atggcagtca ctctcggatc gcaactggcg ctcaccactt
    24721 tggggcatca cttcgtttac cgactgcgta gtgaatttat caagcggatt ctggatacca
    24781 acgtcgagcg cattgaacaa ctcggtagcg cctcgttgct ggcgggatta accagcgatg
    24841 tgcgcaatat caccattgct tttgtgcgtc ttccggaact ggtgcagggg atcattctca
    24901 ctatcggttc cgcggcgtat ctgtggatgc tgtcgggcaa aatgttgctg gtaacggcta
    24961 tctggatggc gatcaccatc tggggtggtt ttgtactggt ggcgcgggtg tacaaacata
    25021 tggcgaccct gcgtgaaacc gaagacaggc tgtatacgga ttttcaaact gtactcgaag
    25081 ggcgcaaaga gctgactctg aaccgggaac gcgccgagta tgtgtttaac aacctctaca
    25141 ttcctgatgc gcaagagtat cgccaccata ttatccgcgc agacaccttc catcttagcg
    25201 ccgtgaactg gtcaaacatc atgatgttgg gcgcaatcgg cctggtgttc tggatggcga
    25261 acagtctcgg ttgggctgat accaacgttg ccgcgaccta ttcgttgacg cttttattcc
    25321 tgcgtacgcc gctgctttcg gcagttggcg cattgccgac gctgctgacg gcgcaggtgg
    25381 cgtttaacaa gctgaacaaa ttcgcgctcg cgcctttcaa agcagagttt ccgcgcccgc
    25441 aggcgtttcc caactggcaa acgctggagc tgcgtaacgt gacgtttgct tatcaggata
    25501 acgcgttttc cgttggtccg attaatctca ccatcaaacg tggcgagctg ctgtttctga
    25561 ttggcggcaa cggtagcgga aaatcgacgc tggcgatgtt gttgacgggc ttgtatcaac
    25621 cacaaagcgg cgaaatcttg ctggatggca aacctgtcag cggcgaacaa ccggaagatt
    25681 atcgcaaact gttttcggca gtgtttaccg atgtctggct gtttgatcaa ctgctggggc
    25741 cggagggtaa acccgctaac ccgcaactgg ttgagaagtg gctggcgcag ctgaaaatgg
    25801 ctcataagtt tgagttaagc aacgggcgta ttgttaacct gaagttatca aaagggcaga
    25861 aaaaacgcgt ggcgctgttg ctggcgctgg cagaagaacg cgatattatc ctgctggatg
    25921 aatgggcggc ggatcaggat ccacacttcc gtcgtgagtt ttatcaggtg ttgctgccgc
    25981 tgatgcagga gatgggtaaa actattttcg ccatcagtca tgatgatcat tactttatcc
    26041 acgccgaccg cctgctggaa atgcgcaacg ggcaacttag cgagctgacg ggcgaagagc
    26101 gcgatgctgc ttcgcgtgat gccgttgccc ggacggcata atattacgac gctgacctgg
    26161 cggactgctg ccgccaggtc aatatgctaa aaatccctt
//
LOCUS       DAZFWP020000065        26060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFWP020000065 DAZFWP020000000
VERSION     DAZFWP020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.065"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHK0Z_RS12265"
                     /old_locus_tag="pgaptmp_002453"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHK0Z_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU4502456.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHK0Z_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHK0Z_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU4502457.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHK0Z_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             complement(941..1408)
                     /locus_tag="ACHK0Z_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4502458.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHK0Z_RS12280"
                     /old_locus_tag="pgaptmp_002456"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHK0Z_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU4502459.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHK0Z_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHK0Z_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU4502460.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHK0Z_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHK0Z_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU4502461.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHK0Z_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHK0Z_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU4502462.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQRLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHK0Z_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHK0Z_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU4502463.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHK0Z_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHK0Z_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU4502464.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHK0Z_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHK0Z_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU4502465.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHK0Z_RS12315"
                     /old_locus_tag="pgaptmp_002463"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHK0Z_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU4502466.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHK0Z_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHK0Z_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU4502467.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHK0Z_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHK0Z_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU4502468.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHK0Z_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHK0Z_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU4502469.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHK0Z_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHK0Z_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU4502470.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHK0Z_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHK0Z_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU4502471.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHK0Z_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHK0Z_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU4502472.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHK0Z_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHK0Z_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU4502473.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHK0Z_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHK0Z_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU4502474.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHK0Z_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHK0Z_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU4502475.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHK0Z_RS12365"
                     /old_locus_tag="pgaptmp_002473"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHK0Z_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU4502476.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHK0Z_RS12370"
                     /old_locus_tag="pgaptmp_002474"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHK0Z_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU4502477.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHK0Z_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHK0Z_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU4502478.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHK0Z_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHK0Z_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU4502479.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHK0Z_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHK0Z_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU4502480.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHK0Z_RS12390"
                     /old_locus_tag="pgaptmp_002478"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHK0Z_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU4502481.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHK0Z_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHK0Z_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU4502482.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHK0Z_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHK0Z_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU4502483.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHK0Z_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHK0Z_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU4502484.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHK0Z_RS12410"
                     /old_locus_tag="pgaptmp_002482"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHK0Z_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU4502485.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHK0Z_RS12415"
                     /old_locus_tag="pgaptmp_002483"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHK0Z_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU4502486.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHK0Z_RS12420"
                     /old_locus_tag="pgaptmp_002484"
     CDS             15548..15916
                     /locus_tag="ACHK0Z_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU4502487.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHK0Z_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHK0Z_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU4502488.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHK0Z_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHK0Z_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU4502489.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHK0Z_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHK0Z_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU4502490.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHK0Z_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHK0Z_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU4502491.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHK0Z_RS12445"
                     /old_locus_tag="pgaptmp_002489"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHK0Z_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU4502492.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHK0Z_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHK0Z_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU4502493.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHK0Z_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHK0Z_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU4502494.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHK0Z_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHK0Z_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU4502495.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHK0Z_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHK0Z_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU4502496.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHK0Z_RS12470"
                     /old_locus_tag="pgaptmp_002494"
     CDS             23095..23637
                     /locus_tag="ACHK0Z_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4502497.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHK0Z_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHK0Z_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU4502498.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHK0Z_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHK0Z_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU4502499.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="ACHK0Z_RS12485"
                     /old_locus_tag="pgaptmp_002497"
     CDS             25034..25291
                     /locus_tag="ACHK0Z_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU4502500.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHK0Z_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             complement(25267..25821)
                     /locus_tag="ACHK0Z_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU4502501.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6696 a         6180 c         6754 g         6430 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagagac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgctaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tttacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcga aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct caacaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcttgcagg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc cgggtttacg tattctgctt atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactaccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atctctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag
//
LOCUS       DAZFWP020000066        25987 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFWP020000066 DAZFWP020000000
VERSION     DAZFWP020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.066"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHK0Z_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /pseudo
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHK0Z_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
     gene            complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHK0Z_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHK0Z_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU4502502.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHK0Z_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHK0Z_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU4502503.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="ACHK0Z_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="ACHK0Z_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU4502504.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="ACHK0Z_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="ACHK0Z_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU4502505.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="ACHK0Z_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="ACHK0Z_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU4502506.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="ACHK0Z_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="ACHK0Z_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU4502507.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLKSAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHK0Z_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHK0Z_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU4502508.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHK0Z_RS12535"
                     /old_locus_tag="pgaptmp_002507"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHK0Z_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU4502509.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9874..10239
                     /gene="yfiL"
                     /locus_tag="ACHK0Z_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             9874..10239
                     /gene="yfiL"
                     /locus_tag="ACHK0Z_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU4502510.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10389..10907
                     /gene="yfiR"
                     /locus_tag="ACHK0Z_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             10389..10907
                     /gene="yfiR"
                     /locus_tag="ACHK0Z_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU4502511.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10897..12123
                     /gene="dgcN"
                     /locus_tag="ACHK0Z_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             10897..12123
                     /gene="dgcN"
                     /locus_tag="ACHK0Z_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU4502512.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12139..12621
                     /gene="yfiB"
                     /locus_tag="ACHK0Z_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             12139..12621
                     /gene="yfiB"
                     /locus_tag="ACHK0Z_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4502513.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12698..13045)
                     /gene="rplS"
                     /locus_tag="ACHK0Z_RS12560"
                     /old_locus_tag="pgaptmp_002512"
     CDS             complement(12698..13045)
                     /gene="rplS"
                     /locus_tag="ACHK0Z_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU4502514.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13087..13854)
                     /gene="trmD"
                     /locus_tag="ACHK0Z_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     CDS             complement(13087..13854)
                     /gene="trmD"
                     /locus_tag="ACHK0Z_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU4502515.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13885..14433)
                     /gene="rimM"
                     /locus_tag="ACHK0Z_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     CDS             complement(13885..14433)
                     /gene="rimM"
                     /locus_tag="ACHK0Z_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU4502516.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14452..14700)
                     /gene="rpsP"
                     /locus_tag="ACHK0Z_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     CDS             complement(14452..14700)
                     /gene="rpsP"
                     /locus_tag="ACHK0Z_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU4502517.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14837..16198)
                     /gene="ffh"
                     /locus_tag="ACHK0Z_RS12580"
                     /old_locus_tag="pgaptmp_002516"
     CDS             complement(14837..16198)
                     /gene="ffh"
                     /locus_tag="ACHK0Z_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU4502518.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16365..17156
                     /gene="ypjD"
                     /locus_tag="ACHK0Z_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             16365..17156
                     /gene="ypjD"
                     /locus_tag="ACHK0Z_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU4502519.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17177..18463
                     /gene="yfjD"
                     /locus_tag="ACHK0Z_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             17177..18463
                     /gene="yfjD"
                     /locus_tag="ACHK0Z_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGU4502520.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            18586..19191
                     /locus_tag="ACHK0Z_RS12595"
                     /old_locus_tag="pgaptmp_002519"
     CDS             18586..19191
                     /locus_tag="ACHK0Z_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HGU4502521.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(19226..19816)
                     /gene="grpE"
                     /locus_tag="ACHK0Z_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             complement(19226..19816)
                     /gene="grpE"
                     /locus_tag="ACHK0Z_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU4502522.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            19939..20817
                     /gene="nadK"
                     /locus_tag="ACHK0Z_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             19939..20817
                     /gene="nadK"
                     /locus_tag="ACHK0Z_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU4502523.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20903..22564
                     /gene="recN"
                     /locus_tag="ACHK0Z_RS12610"
                     /old_locus_tag="pgaptmp_002522"
     CDS             20903..22564
                     /gene="recN"
                     /locus_tag="ACHK0Z_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU4502524.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22713..23054
                     /gene="bamE"
                     /locus_tag="ACHK0Z_RS12615"
                     /old_locus_tag="pgaptmp_002523"
     CDS             22713..23054
                     /gene="bamE"
                     /locus_tag="ACHK0Z_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU4502525.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(23116..23406)
                     /gene="yfjF"
                     /locus_tag="ACHK0Z_RS12620"
                     /old_locus_tag="pgaptmp_002524"
     CDS             complement(23116..23406)
                     /gene="yfjF"
                     /locus_tag="ACHK0Z_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU4502526.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(23396..23833)
                     /gene="ratA"
                     /locus_tag="ACHK0Z_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             complement(23396..23833)
                     /gene="ratA"
                     /locus_tag="ACHK0Z_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU4502527.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            24004..24486
                     /gene="smpB"
                     /locus_tag="ACHK0Z_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             24004..24486
                     /gene="smpB"
                     /locus_tag="ACHK0Z_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU4502528.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24701..25063
                     /gene="ssrA"
                     /locus_tag="ACHK0Z_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     tmRNA           24701..25063
                     /gene="ssrA"
                     /locus_tag="ACHK0Z_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            25298..>25987
                     /locus_tag="ACHK0Z_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             25298..>25987
                     /locus_tag="ACHK0Z_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcoRII N-terminal effector-binding
                     domain-containing protein"
                     /protein_id="HGU4502529.1"
                     /translation="MSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNI
                     VEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQARAIYYNNRYFGKTRNEKRITRWGR
                     GSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTLIS
                     GPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLID
                     RRRVEYDIFLLV"
BASE COUNT         6667 a         6799 c         6078 g         6443 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct cagttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg
     1981 cggcggcctt tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattag ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cacccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaaact cacgccgccg
     3481 atacgagaag agctacaggc cgcaacacct tttggtagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaactta cgcagcatgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 tgggttcatc tactgtaccg ccagcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgtggta
     4141 catcggcaat gggcggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cgttgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggaa tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa tacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattccca tcacacattt tgacatcagg agcggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt ggccgtcgag
     6361 gtaggaaaag ccaaactgct ctcgcatcgc ccggtacgag atattgatcg tgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacga ctgatttatc caccattaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatcct cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc attgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcaa
     7081 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     7141 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtgc tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttaa atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccagatgca gcccataagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgagagggtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tagctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagcagccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc gcgatttcaa cccttacttc tgtatagata aatttacact tcctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
    10201 ggggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt
    10261 aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacgatc gcatgaaaaa
    10321 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
    10381 atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag
    10441 caccaatact cgctcaggag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
    10501 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
    10561 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
    10621 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
    10681 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
    10741 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
    10801 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
    10861 tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga taattctctt
    10921 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
    10981 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
    11041 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc
    11101 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa
    11161 ttttcaactg cagaagtacg tgataagcag caaaatattc tggcgtcctg gcattacacc
    11221 cgtaaggatc caggcgatac tttcagcaat ttcataagcc actggctctt ccccgccccc
    11281 atcattcagc cgattcgtca caatggtgaa accattggcg aagtacgctt aaccgctcgc
    11341 gacagttcaa tcagccattt tatctggttt tcgctcgccg tactgaccgg ttgtattctg
    11401 ctggcatcag gcatcgcaat taccctcacc cgccatttgc acaatggcct ggtagaagcg
    11461 ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga
    11521 gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc tcgacttcaa cagtctgctg
    11581 gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct tctgcgtacc
    11641 gcgctacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag cggcatcaat
    11701 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
    11761 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagagt cttgattgaa
    11821 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
    11881 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca gcagatatgc
    11941 tcagcactga cacaaatctt taatctccct tttgatcttc ataatggcca tcagaccacc
    12001 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
    12061 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
    12121 taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcacta
    12181 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
    12241 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
    12301 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
    12361 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
    12421 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
    12481 gcatgggcta tgggtggaca aattccacgc agcaatctca ccacacaggg tttaggaaaa
    12541 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
    12601 gtggtgatta caacccctta aaacacgagc aaacagacaa aaaaagggcc agccaattgg
    12661 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
    12721 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
    12781 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
    12841 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
    12901 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
    12961 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
    13021 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
    13081 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
    13141 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
    13201 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
    13261 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
    13321 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
    13381 tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
    13441 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
    13501 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca gttcgctgac
    13561 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
    13621 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
    13681 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
    13741 aggactccaa ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
    13801 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
    13861 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
    13921 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
    13981 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
    14041 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
    14101 caggtctttc cagtagtagt caccctcttc aagctgaggc agctgcgatg aatccacgac
    14161 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
    14221 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
    14281 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
    14341 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
    14401 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
    14461 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
    14521 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
    14581 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
    14641 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
    14701 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
    14761 attaaaagcc cgaaaatttt actgatttct ggagaaaatg caatcagcag ttaataactc
    14821 tgtcgcataa ggtcatttag cgaccaggaa atcccggcgg catcatcccc ttcatgcttc
    14881 tcatcatctt cgccattccg cctttcttca ttttcttcat catgcgctgc atgtcgtcga
    14941 actgcttcag aaggcggttc acgtcctgca cctgcatacc gcaaccggca gcgatacgac
    15001 gcttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga
    15061 tgatggcttc catacgcacc agcactttat cgtccatctg cgacttaaca ttatccggga
    15121 tctgccccat gcccggcagc ttgcccatca ggctagccat gccgcccata tttttcatct
    15181 ggcgcagttg ctcaagaaaa tcgttgagat cgaagccgtc gccttttttc agcttgctgg
    15241 ctaatttctc tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc agcgacagta
    15301 cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc tccagcgcct
    15361 cagttttctc gccaacaccg agaaacttga tcggtttgcc agtgatgtga cgaatagaga
    15421 gcgccgcacc gccgcgggca tcgccgtcca ctttggtcaa cactacgccg gtaagcggta
    15481 acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
    15541 caaacagggt ttcaaccggg ttaatcgacg catggacttg tttgatctcg tccatcatcg
    15601 cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag aatttcagtt
    15661 tggcttcttt cagcgccgcg ttaacgatat ctaccggctt ctgaccaaca tcagaaggga
    15721 agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat
    15781 aaacgtcggc agaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga
    15841 gtttaccaac gctggttgtt ttaccggcac cttgcaggcc cgccatcagt acgaccgcag
    15901 gcggttgcgc agccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt
    15961 tacggactat tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa
    16021 ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg acgtccgcct
    16081 ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca gtgaggcgtc
    16141 cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg
    16201 tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg
    16261 cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg
    16321 ctatactgct tctctttctt attgctcaaa ctgtcgacat tactatgccc gtttttgctc
    16381 tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa
    16441 aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg gtctgccacg
    16501 caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc
    16561 tgaacgttgg ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc
    16621 gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc aacctggcgc
    16681 tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct gggatgctgg
    16741 tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc ctgtacgcgc
    16801 tgcaactggc gtggattgat taccaactga agaacaagaa gctggcattt aaccaggaaa
    16861 tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt ggcgtggtgc
    16921 tgctaacgct cacgctttgc actggcctgt tctacatgca caacctattt agcatggaaa
    16981 atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg
    17041 gacattacca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggcgcgg
    17101 taattctgac actggcctac ttcggcagcc gaattgtcca gcagttaatc agctaaaccc
    17161 agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
    17221 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
    17281 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
    17341 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
    17401 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
    17461 aattgcgact ggtgtgctga cttttgtcgt actggtattt gctgaggtat tgccgaaaac
    17521 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctccgct
    17581 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
    17641 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
    17701 cactatcgtg cacgaatcac gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
    17761 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
    17821 cggtattgat atcaacgatg actggaaatc gattctgcgc caactctccc actcacctca
    17881 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
    17941 tgaagcctgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
    18001 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtaaagtt
    18061 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
    18121 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
    18181 aacacttgcc gaagaggtca cgccgcaaaa cgacggttcg gtgattatcg atggcaccgc
    18241 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
    18301 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca cccgcgtgcg
    18361 tattggcgag tacgatatag atattctcga cgtacaggac aatatgatta agcaggtaaa
    18421 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgcaatg gcgaactccg
    18481 gttcgccttt tttttatcgc cgtcttttag gattccatca attcctgtca tttacttagc
    18541 aatgtaataa tgtcttttca agcattcgga aaggatattg atgttatgtc agaagattat
    18601 gtaattgaat gggataagga ttttgcagac gatctcaacg tcgtcgccag cgttttttta
    18661 cagcacaccc caaccttatg gcccactatt ttctctcaac tttcgacgca acctgaaatc
    18721 tttgaagacg aagatgaaga cgaatatgga ttgcaggacg ttctcgattg cagtggtggc
    18781 gatctcggga atcgtgacct ggcgcaggca tttttacagg ttctacgcgg tgaaggatta
    18841 attcaactcg ttgactggaa aggtgaagat gaagaaggtg agctggctaa ttttgctgca
    18901 gatcgctttt atgagctcac aaaaaacctg atagcttctg aggaattaag gagtcttctt
    18961 gtcgaaataa cccaagagga tgaaatctcc gatgtctgtg aagccggaga ccgctacctt
    19021 gacgaaatct ttgaacgcat ccaaaccgaa ctcaataaaa ggggctttca gatttttgat
    19081 ctgaatgaag gatctgacac ctataacgtg gtcgttctgc caatgaacga atataaaaaa
    19141 atagatgatt tcaacacgcc gtggctggaa gtacaggatt ttttaagcta aaaaaatggc
    19201 cgacttcgcg tcggccatca gatatttact tcgccttcgc tacagtaacc atcgccgcac
    19261 gaatcgtacg accattcagc gtataaccct tctgcataat gcccagtacg ttacctggcg
    19321 caacgtcatc agattccacc attgcgatag cctgatgcac attcgggtcc agcggcacgt
    19381 tagtttcggc gatcacttca acaccaaact tacgcacaac atccagcatc gacttcagcg
    19441 tcagctcaat gccttcaacc atcgcagaca tatccgggtt agctttatca gccacttcca
    19501 gcgcacgatc caggctatca atcaccggca gcaattcgtt gatgaatttc tccagcgcga
    19561 atttgtgggc tttttcaata tccagttcag tacgtcgacg caggttttcc atttcggctt
    19621 ttacacgcaa aatgccgtca cgttcacggg tctgggcttc agccagctga gcttcgagat
    19681 tcgcaatttt ttcatcgcgc ggatccacct gctcagcaga agcttctggc tcaactgcct
    19741 caatctcttc gtgctgatcc atgataattt cttccggggc ttgcccctca ggcgttttct
    19801 gttctttact actcatgaat ttctccgcgt ttttttcgca ttcatctcgc taacttcgct
    19861 tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc
    19921 aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt gggacaccca
    19981 cggcacccca ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt
    20041 tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa tgtgaaaact
    20101 ggcacgctcg cggagattgg gcaactagct gatctcgcgg tagtcgttgg tggcgacggt
    20161 aatatgctgg gcgcggcacg cacactcgcc cgttacgata ttaaagttat tggaatcaac
    20221 cgtggcaacc tgggtttcct gactgacctt gaccccgata acgcccagca acagttagcc
    20281 gatgtgctgg aaggccacta catcagcgag aaacgttttt tgctggaagc gcaagtctgt
    20341 cagcaagatt gtcagaaacg catcagcacc gcgataaatg aagtggtgct tcatccaggc
    20401 aaagtggcgc atatgattga gttcgaagtg tatatcgacg agatctttgc gttttctcag
    20461 cgatctgatg gactaattat ttcgacgcca acaggctcca ccgcctattc cctctctgca
    20521 ggcggtccta ttctgacccc ctctctggat gcgattaccc tggtgcccat gttcccgcat
    20581 acgttgtcag cacgaccact ggtcataaac agcagcagca cgatccgtct gcgtttttcg
    20641 catcgccgta acgacctaga aatcagttgc gacagccaga tagcactgcc gattcaggaa
    20701 ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca tccgaaagat
    20761 tacagttatt tcaacacatt aagcaccaag ctcggctggt caaaaaaatt attctaattt
    20821 tacgccagcc tctttactgt atataaaacc agtttatact gtacacaata acagtaatgg
    20881 tttttcatac aggaaaacga ctatgttggc acaactgacc atcagcaact ttgctatcgt
    20941 tcgtgagctt gagattgatt ttcatagcgg catgaccgta ataactggcg agaccggcgc
    21001 gggtaaatct attgcaatag atgccctcgg tctttgtctc ggtggtcgcg ctgaagccga
    21061 catggtgcgt accggcgctg ctcgcgctga cctgtgcgcc cgtttttctc tgaaagatac
    21121 gccagcggct ctgcgctggc tggaagaaaa ccagcttgaa gacgggcatg aatgtttgct
    21181 tcgtcgcgtg atcagcagcg atggtcgctc ccgtggtttc atcaacggta cagctgttcc
    21241 tctgtcacaa ctgcgcgaac tgggtcagtt gctgattcag atccatggtc agcacgctca
    21301 tcagttactc accaaacccg agcaccaaaa gttcctgctt gatggctatg ccaatgaaac
    21361 ctctctactc caggaaatga cagcacgtta tcagttgtgg catcaaagct gccgtgacct
    21421 cgcgcatcat caacagttaa gtcaggaacg cgctgcccgt gcggaactgc tgcaatacca
    21481 attaaaagaa cttaacgaat ttaatccgca gcccggagag tttgaacaaa tcgacgaaga
    21541 gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc agccagaatg cattggcatt
    21601 aatggccgac ggtgaagacg caaacctgca aagtcagctt tacacggcta aacaactggt
    21661 gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc tggaagaagc
    21721 taccatccag attgctgaag ccagcgatga actgcgccac tactgcgatc gtctggatct
    21781 cgatcccaac cgactatttg aacttgaaca gcgcatctca aaacagattt cgctggcacg
    21841 taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac tggaagaaca
    21901 gcagcaactg gacgatcagg ccgactcaca agaaacgctt gcgctggcgg taacgaaaca
    21961 tcatcagcag gcactggaaa tcgcgcgcgc attacaccaa caacgccagc aatatgcaga
    22021 agaacttgca cagctgatca ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt
    22081 tacgatcgat gttaaatttg acgagcatca cctgggcgct gacggtgccg atcgtattga
    22141 gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag tcgcatccgg
    22201 tggtgaattg tcccgcatcg cactggcaat ccaggtcatc acggcgcgta aaatggaaac
    22261 cccggcactg atttttgatg aagtggatgt agggattagc ggtccaacag cggcagttgt
    22321 cggcaaactg ctgcgtcaac tcggcgaatc aactcaggtg atgtgtgtta cccacctgcc
    22381 acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg atggtgcgat
    22441 gacagaaacg catatgcaat ccctgaataa aaaagcgcgg ttacaagagc tggcgcgcct
    22501 gcttggtggc agtgaagtca cacgtaatac actggcgaat gcgaaagaac tgcttgcagc
    22561 gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa gaccggaaag
    22621 caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga tgagccacgt
    22681 actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg actgctgcag
    22741 cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg gtttaccgtc
    22801 ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt atccaaaata cgtgttggca
    22861 tgacgcaaca acaagttgcg tacgcattgg gtacaccgct gatgtccgat ccatttggta
    22921 cgaatacctg gttctatgtc ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa
    22981 cgctgacgct gacctttaac agtagcggtg tgttgaccaa tattgataac aaacctgcgc
    23041 tgagtggtaa ctaataataa agttgctctc aaagacgtta aaaaaggtgc tcaatgagca
    23101 ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc gaagctcttt
    23161 cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca cactatcgct
    23221 tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga tatcggtacg
    23281 caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt
    23341 cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg caattttacc
    23401 tggcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta gacgccagct
    23461 ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca aagtcgagat
    23521 gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca ccaatcaatt
    23581 tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc aactggttgc
    23641 gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca gtcatctgcc
    23701 caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga ggataagact
    23761 gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt gcggtccggc
    23821 taatctgagg cataacaatt tccatcaaca aaaatccaac aaataatatc atttatcctg
    23881 ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa gatagagcct
    23941 tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg attcacgacg
    24001 cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct taacaagcgc
    24061 gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct gcaaggctgg
    24121 gaggttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt ccttctgcgt
    24181 gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc ctccacgcat
    24241 gtggtgtgcg atcctacccg tacccgcaag ttacttctca accaacgcga actggactca
    24301 ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct gtactggaaa
    24361 aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca cgataaacgt
    24421 tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa aaacgcccac
    24481 cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc tccggcggcg
    24541 cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc attgcttaat
    24601 cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat tgaggctggt
    24661 catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt ctggattcga
    24721 cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa
    24781 aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct
    24841 ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca aaagagatcg
    24901 cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc
    24961 gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag taccgaggat
    25021 gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaatcatga ttggacggtg
    25081 taaggacaac accaacaaaa acaggaagtt agcagtctca gcaggacacc gaccagacgg
    25141 tgaggagata aaaaaggata cgcaaaggag ccgtggctct cgagtgacac aaaagcccgc
    25201 ttatgcgggc tttttgtttt tcccttaagt tcctagcctc ttatctataa ctatggaaaa
    25261 atgttaaccc tgactgtatg ttaacaaagg gatgtatatg tcggtttttc ataactggct
    25321 gcttgatatc gcaagcggga attactttat ctacatcaaa cgcctttctg caaacgacac
    25381 aggcgcaaca ggtggccatc aggtcggact ttatatcccc tcaaatatcg ttgaaaagct
    25441 ttttccatct atcaatcata ctcgcgaact gaacccttca gtcttcctta ctgcgcatgt
    25501 atcatcccat gattgccctg atacccaagc acgcgcaatt tattacaaca accgttattt
    25561 tggtaagacc cgaaacgaaa aaagaattac gcgctggggg agaggaagtc cattacagaa
    25621 ccctgaaaat acaggagccc tcacaattct tgctttcagg ttaaacgaac agaacactga
    25681 ctgttccgag gtagatatat gggtctgcgt caatcccgat gaagaggata tcatcgagtc
    25741 tgctattggc gaaatcatac ctggaaccct aatttccggc cctgccggac aaattttggg
    25801 cggattgtct cttcagcaaa ctccagtaaa tcataaatat gttattcctg aagactggaa
    25861 gaagcgtttt ccttctggaa acgaaattat tcaatatgct gctggccatt atgctaaaaa
    25921 ctccaaggat ccagatgagc aactgattga ccgtcggcgt gtcgagtatg atatttttct
    25981 actcgtc
//
LOCUS       DAZFWP020000067        25469 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFWP020000067 DAZFWP020000000
VERSION     DAZFWP020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.067"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            98..859
                     /gene="adiY"
                     /locus_tag="ACHK0Z_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             98..859
                     /gene="adiY"
                     /locus_tag="ACHK0Z_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU4502530.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            996..2333
                     /gene="adiC"
                     /locus_tag="ACHK0Z_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             996..2333
                     /gene="adiC"
                     /locus_tag="ACHK0Z_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU4502531.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2437..4080
                     /gene="eptA"
                     /locus_tag="ACHK0Z_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     CDS             2437..4080
                     /gene="eptA"
                     /locus_tag="ACHK0Z_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU4502532.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4077..4745
                     /gene="basR"
                     /locus_tag="ACHK0Z_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             4077..4745
                     /gene="basR"
                     /locus_tag="ACHK0Z_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU4502533.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4746..5846
                     /gene="pmrB"
                     /locus_tag="ACHK0Z_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             4746..5846
                     /gene="pmrB"
                     /locus_tag="ACHK0Z_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU4502534.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5822..5911)
                     /gene="pmrR"
                     /locus_tag="ACHK0Z_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             complement(5822..5911)
                     /gene="pmrR"
                     /locus_tag="ACHK0Z_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU4502535.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6023..7525)
                     /gene="proP"
                     /locus_tag="ACHK0Z_RS12675"
                     /old_locus_tag="pgaptmp_002535"
     CDS             complement(6023..7525)
                     /gene="proP"
                     /locus_tag="ACHK0Z_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU4502536.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7789..8667)
                     /gene="rdcB"
                     /locus_tag="ACHK0Z_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             complement(7789..8667)
                     /gene="rdcB"
                     /locus_tag="ACHK0Z_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU4502537.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRKHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8664..10892)
                     /gene="crfC"
                     /locus_tag="ACHK0Z_RS12685"
                     /old_locus_tag="pgaptmp_002537"
     CDS             complement(8664..10892)
                     /gene="crfC"
                     /locus_tag="ACHK0Z_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU4502538.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11294..11629
                     /gene="yjdM"
                     /locus_tag="ACHK0Z_RS12690"
                     /old_locus_tag="pgaptmp_002538"
     CDS             11294..11629
                     /gene="yjdM"
                     /locus_tag="ACHK0Z_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU4502539.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11789..12232
                     /gene="yjdN"
                     /locus_tag="ACHK0Z_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             11789..12232
                     /gene="yjdN"
                     /locus_tag="ACHK0Z_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU4502540.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12365..13153
                     /gene="phnC"
                     /locus_tag="ACHK0Z_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             12365..13153
                     /gene="phnC"
                     /locus_tag="ACHK0Z_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU4502541.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            13178..14194
                     /gene="phnD"
                     /locus_tag="ACHK0Z_RS12705"
                     /old_locus_tag="pgaptmp_002541"
     CDS             13178..14194
                     /gene="phnD"
                     /locus_tag="ACHK0Z_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4502542.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14300..15079
                     /gene="phnE"
                     /locus_tag="ACHK0Z_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             14300..15079
                     /gene="phnE"
                     /locus_tag="ACHK0Z_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU4502543.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15100..15825
                     /gene="phnF"
                     /locus_tag="ACHK0Z_RS12715"
                     /old_locus_tag="pgaptmp_002543"
     CDS             15100..15825
                     /gene="phnF"
                     /locus_tag="ACHK0Z_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU4502544.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15826..16278
                     /gene="phnG"
                     /locus_tag="ACHK0Z_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             15826..16278
                     /gene="phnG"
                     /locus_tag="ACHK0Z_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU4502545.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16275..16859
                     /gene="phnH"
                     /locus_tag="ACHK0Z_RS12725"
                     /old_locus_tag="pgaptmp_002545"
     CDS             16275..16859
                     /gene="phnH"
                     /locus_tag="ACHK0Z_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU4502546.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16859..17923
                     /gene="phnI"
                     /locus_tag="ACHK0Z_RS12730"
                     /old_locus_tag="pgaptmp_002546"
     CDS             16859..17923
                     /gene="phnI"
                     /locus_tag="ACHK0Z_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU4502547.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            17916..18761
                     /gene="phnJ"
                     /locus_tag="ACHK0Z_RS12735"
                     /old_locus_tag="pgaptmp_002547"
     CDS             17916..18761
                     /gene="phnJ"
                     /locus_tag="ACHK0Z_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU4502548.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            18758..19516
                     /gene="phnK"
                     /locus_tag="ACHK0Z_RS12740"
                     /old_locus_tag="pgaptmp_002548"
     CDS             18758..19516
                     /gene="phnK"
                     /locus_tag="ACHK0Z_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU4502549.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19627..20307
                     /gene="phnL"
                     /locus_tag="ACHK0Z_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     CDS             19627..20307
                     /gene="phnL"
                     /locus_tag="ACHK0Z_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU4502550.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            20304..21440
                     /gene="phnM"
                     /locus_tag="ACHK0Z_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             20304..21440
                     /gene="phnM"
                     /locus_tag="ACHK0Z_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU4502551.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAL
                     CRVRQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            21440..21997
                     /gene="phnN"
                     /locus_tag="ACHK0Z_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             21440..21997
                     /gene="phnN"
                     /locus_tag="ACHK0Z_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU4502552.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            21984..22418
                     /gene="phnO"
                     /locus_tag="ACHK0Z_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             21984..22418
                     /gene="phnO"
                     /locus_tag="ACHK0Z_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGU4502553.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22420..23178
                     /gene="phnP"
                     /locus_tag="ACHK0Z_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             22420..23178
                     /gene="phnP"
                     /locus_tag="ACHK0Z_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU4502554.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTQKFLLNNHPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIHSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(23325..23654)
                     /gene="yjdP"
                     /locus_tag="ACHK0Z_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     CDS             complement(23325..23654)
                     /gene="yjdP"
                     /locus_tag="ACHK0Z_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU4502555.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23723..24172)
                     /gene="rpiB"
                     /locus_tag="ACHK0Z_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /gene_synonym="alsI"
     CDS             complement(23723..24172)
                     /gene="rpiB"
                     /locus_tag="ACHK0Z_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HGU4502556.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQWRN"
     gene            24531..25421
                     /gene="alsR"
                     /locus_tag="ACHK0Z_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             24531..25421
                     /gene="alsR"
                     /locus_tag="ACHK0Z_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HGU4502557.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALSVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT         6019 a         7102 c         6680 g         5668 t
ORIGIN      
        1 tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc
       61 gagaactggt cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc
      121 ttgtattgtt ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat
      181 tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc
      241 ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg
      301 gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact
      361 caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc
      421 cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg
      481 ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag
      541 tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt
      601 agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac
      661 cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat
      721 ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt
      781 tatttctgtc tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag
      841 agaacgcact gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag
      901 cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt
      961 attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca
     1021 aagtgggctt aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt
     1081 ttctgttacc tgcaaacctg gcctcgactg gcgggattgc catttatgga tggttggtga
     1141 cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgatccaa
     1201 gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa
     1261 ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg
     1321 taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct
     1381 gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc
     1441 gtgtgcaggc agttgccacc gtgctggcgc tgatccccat cgtcgggatt gccgtatttg
     1501 gctggttctg gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca
     1561 ccttcggtgc aattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa
     1621 gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca
     1681 ttggtggggt attgattgcc gccgtttgct atgtactttc taccaccgcg attatgggga
     1741 tgatccctaa tgccgcactg cgcgtttctg cttcgccatt cggtgatgcc gcacggatgg
     1801 cgttgggtga caccgccggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt
     1861 cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac
     1921 tgttcccacc gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta
     1981 tcgtcggtat tttgatgacc atattccagc tcagcagcat ttcaccaaac gcgaccaaag
     2041 agttcggtct ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct
     2101 gtgcggcgtt actgctgctc ggacacggtc actttggtaa agcacgcccg gcatatctgg
     2161 cagttactac cattgccttc ctctactgca tctgggccgt ggtggggtcc ggagcgaaag
     2221 aggttatgtg gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca
     2281 accggctaca taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct
     2341 taatccagca aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc
     2401 gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga
     2461 ccctctttga atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg
     2521 aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta
     2581 ctggttttct tgtcgatgcc ggtcgtcgct ttcagcgtga ttaatattgt cctgacacta
     2641 agctctttct tatggcttaa tcgaccactg gcctgcctgt ttattctggt tggcgcggct
     2701 gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt
     2761 attgatacca ctccggcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg
     2821 ggattcagcg gcgtgcttgc tgcgctgatt gcctgctgga taaaaatcaa acctgccacc
     2881 tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt actactgatt
     2941 ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag
     3001 ctggtgaaat ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat
     3061 cagcgactgg caaatctacc gctggtgcga attggtgaag acgcccaccg caacccgtta
     3121 atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg
     3181 gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc gaaagataac
     3241 gtggtctatt tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc
     3301 atgttctcgg atatgccgcg tgagcattac aaagaagagc tggcacagca ccaggaaggc
     3361 gtgctggata tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc
     3421 tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctggt
     3481 cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc
     3541 aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc
     3601 tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag
     3661 atccagacct gtagcaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc
     3721 gactatattg ttgataaagc gattaatctg ctgaaagaac atcaggataa atttaccacc
     3781 agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac
     3841 ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccgatgct gctgtggctg
     3901 tcggaggatt atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa
     3961 acgcaacact attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag
     4021 acgaagtatt accaggctgc ggatgatatt ctgcaaactt gcaggagagt gagtgaatga
     4081 aaattctgat tgttgaagac gatacgctgt tattgcaggg attgattctg gcggcgcaaa
     4141 ctgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg
     4201 ccggtcatta cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt
     4261 ttctcgcccg tatccggcag aaaaaataca ccctgccagt actgatcctc accgctcgcg
     4321 atacgctgac cgacaaaatc gctgggctgg atgtcggtgc cgatgactat ctggtgaagc
     4381 cttttgcgct ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc
     4441 agggcgaaag tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat
     4501 ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa
     4561 tgctcaaagc aggcagtccg gtgcatcggg aaattctcta caacgacatc tataactggg
     4621 acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg
     4681 gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa
     4741 actaattgaa tctgatgcat tttctgcgcc gaccaatatc gctgcgccaa cggctgatat
     4801 tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg
     4861 aaagtaccga gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc
     4921 gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta
     4981 tggtcagcct gacgctattt atctgctatc aggcggtgcg ccgcatcacc cgcccgctgg
     5041 cggagctgca aaaagagctg gaagcgcgca ctgccgacaa cttaacgccc attgccattc
     5101 acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt aaacgatctg gtcagtcgcc
     5161 tgaccagcac gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa
     5221 cgccactggc gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg
     5281 tagcaccgtt agtggcacga cttgatcaga tgatggagag cgtctcccag ctgctgcaac
     5341 tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag
     5401 atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc
     5461 tgctattgcc agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga
     5521 tgttattgcg aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta
     5581 tgattaagct gcaagaagat gacggagcgg tcatggcagt tgaagatgaa ggaccaggta
     5641 ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg
     5701 gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt
     5761 ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag
     5821 atcagaacgt ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg
     5881 tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga
     5941 gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc
     6001 acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg
     6061 tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat
     6121 tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg
     6181 cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca
     6241 ccgccactac catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc
     6301 aggccgccag tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca
     6361 gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgcccg
     6421 taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt
     6481 tactgttaat cagaataaac gccgggatcg ccaacacaaa cagggcaaca ctaccaagta
     6541 gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca
     6601 gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt
     6661 tatgcgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa
     6721 ttaccagacc aatacatgtc aacaggctgc gccagtgttt ggtggcaatc tctttaaacg
     6781 agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcgacatgct
     6841 gctggaacgc cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg
     6901 gcagagcaat aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa
     6961 tggtcgaaat taacaccact acgccagcac ccagcacaaa cccggcaata gaaccgaagt
     7021 ccagccagct gcccataaag ccacgtttac ggtcaggtga gtattccgca acaaatatcg
     7081 acgccccggt atattcaccg ccgaccgaga aaccttgtgc catcttacag atcaacagca
     7141 gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac
     7201 tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca
     7261 acataccaaa gaagagcccg ccaagcggtc gaatcagaaa gggaacggag aacgtggcaa
     7321 gtgcagcaac catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt
     7381 aagcaacaaa accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg
     7441 cggtaatggc tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaatcg
     7501 gttttacttt tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac
     7561 gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct
     7621 gtaacaagcg tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt
     7681 tgacgcctga attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc
     7741 aacttcacac atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt
     7801 tacagagaag atttcctcga ccattgcccc cgccagacgc tgcgctgaac agagtcgtgg
     7861 catccccagc gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc
     7921 tgccgtggtc cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac
     7981 ggtcgaagag gcaaaccact gatggtaata gtgacgcgtg gtcggggccg cattgatact
     8041 ctctgccaga tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg ccaccgcttg
     8101 cgggcttccc agacgacagg catcgccaaa taccccccaa cgcaactctt cgagaacgat
     8161 aaaacagcgc ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat
     8221 ttgctcgctc tgttcacccg cctgcaccag taggcctcgt tgcgttaatg ccttttcctt
     8281 ccagcgacaa cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg
     8341 gaaagcagag tgcttgcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg
     8401 cgtcagcgcc agatgaccgg gatccagcat cgacgccagt ttttccacca ttgtcagtcg
     8461 caggctaagc ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacacgcga
     8521 aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg gaaggcgggc
     8581 atcatcgccc tgcgccagat ccactaaaaa gcggggataa accgactcca gcacgcgacc
     8641 ggggccgtcc agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca
     8701 tcgcgtaaca gctgggcgtc ggtgtaaatc cagccgtgag tcatcacagt ctgctgcaat
     8761 tgctgctgca tcagcctgac caccgattca ttttgttgac gcagagccag gctttcgcgt
     8821 aaacgcgtct gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct
     8881 gccgtaacag attcatcgac ctgctctgcc agaactttag ccatttgttg gcaataaaga
     8941 tcgacttctg cgcttaatgc tcgttgcaac acactgtaat caaccgtttc tgtcggggat
     9001 ttctcatttc cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca
     9061 acgcctaaac gcgtgctctg ctcgtccgtt gcatcctggc gcgaaatgac tgcactgaac
     9121 agctggcgag tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga
     9181 cgaaaccctg actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg
     9241 gttaacaccc ggcaaagggt ggattccagc tcggcaaaac gcagcgtaaa gtcgcgactg
     9301 atggtgttct gcgccgtctg taacagtgtc tcacagcggg tacgcatctc gcttaacggc
     9361 tccgaatcat cctgaaacaa ggcggctaac tgcgcattca gcgcatcttg ttgttgacgc
     9421 agaaagtggt tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc
     9481 tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc
     9541 aattgttcac aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc
     9601 tgcgcgtaat tcaacagttt atgcgcagca gatcgcaaag catacaacga ggcgttagcg
     9661 taagcggcat gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga
     9721 tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg
     9781 agcgcggcat gggcaaaatc ttccacccag cgttgttgct ctggcgctgg taacttaccg
     9841 ttgttggcta actcatggcg cgcccgattc gccaggtagc cccacatcga cgacaccgga
     9901 aatatctgct gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc
     9961 tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc
    10021 ggcaccgatt gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc
    10081 agttgcgtat aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc
    10141 attttttgca gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac
    10201 tgaccgggat aactctccag ccccgccaga tggacaaact ccacttcaat cacgggaata
    10261 tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc
    10321 actaaatcat tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt
    10381 tcgaaagcga cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc
    10441 agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt
    10501 aaacaatcga ttggcgcgac atgtgaaaaa tgcagtaccg gttccttttg cccgggcgta
    10561 tggcgaataa gcgtcggcag cgcagtcatt gggcgattac gattaggcag aacctccgta
    10621 ccaacaatgg cattaatggt ggttgatttc cctgctttca tggtaccgac aattgccagc
    10681 accatttcca gtcgggaaat tttacgcaac tcattattca gcatcgcgtg acgttcggcg
    10741 atattaggct gactccaggg taaagccagt tgtggcgcgt cgtctccggg tacagagaga
    10801 ggcatttttt ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc
    10861 tgacttaact catacagggt ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg
    10921 ttacttttat ttagccagat tgtttttgag ttctgttttc ggcttttata attactgcaa
    10981 gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgcc ttctgatacg
    11041 tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa gtagagcatt
    11101 aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc gtttcctttc
    11161 aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag ggattttgtg
    11221 tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc tgctgacttt
    11281 tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat acacttacga
    11341 agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg cagaacctgc
    11401 acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg ctgacggcga
    11461 cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg
    11521 caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt gcaaaatcga
    11581 cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat tgtattgtga
    11641 tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact gcctgatgcg
    11701 acgctgaccg cgtcttatca ggcttctctc tcatctgtat aaatttcgaa ctacacttaa
    11761 ctggcttctc ttaactgagg tcaccatcat gccgttaagt ccctacctct cttttgccgg
    11821 taactgtgcc gacgcaattg cttattatca acgtacgttg ggcgctgaac tgctctataa
    11881 aatcagcttc ggcgaaatgc caaaatcagc gcaggacagc gccgagaact gcccttccgg
    11941 aatgcaattt cccgataccg ccatcgccca cgccaacgtg cgcattgccg gaagcgacat
    12001 catgatgagc gatgctatac cgtcaggaaa agccagctac tccggcttta cgctggtgct
    12061 cgattcgcaa caggtcgaag aaggaaaacg ctggtttgac aatcttgccg ctaacggaaa
    12121 aatcgaaatg gcctggcagg aaactttctg ggcgcatggc tttggcaaag tcaccgataa
    12181 atttggcgta ccgtggatga ttaatgtcgt caaacaacaa ccaacgcaat aacccgccgg
    12241 gaggcccgcc ctcccgcact gtcatcgaat tcccgttaac tcttcatctg ttagtcactt
    12301 ttaattaacc aaatcgtcac aataatccgc cacgatggag ccactttttt agggaggctg
    12361 catcatgcaa acgattatcc gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc
    12421 gctgcatgcg gttgatctga acattcatca cggtgaaatg gtggctctgc ttgggccgtc
    12481 gggttccggc aaatccaccc ttttacgtca cttaagcggt ttgattaccg gcgataaatc
    12541 cgccggcagc catatcgagc tgctgggccg cacagtccag cgcgaaggcc gtctggcgcg
    12601 cgatatccgc aaaagccgcg ccaacaccgg ctacatcttc caacaattca acctggtgaa
    12661 ccgcctgagc gtactggaga acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg
    12721 cacctgtttt agctggttca ccggcgagca gaaacagcgc gcgttacagg cgctgacccg
    12781 cgttggcatg gtgcattttg cccatcagcg cgtttccacc ctctccggcg gacagcagca
    12841 gcgtgtggcg attgcccgcg cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc
    12901 catcgcctcg ctggacccgg aatccgcccg catcgtgatg gacaccctgc gcgacatcaa
    12961 tcagaacgac ggcatcaccg tggtcgtcac gctgcatcag gtggattacg ccctgcgcta
    13021 ctgcgaacgc atcgtcgccc tgcgccaggg gcacgttttc tacgacggca gcagccaaca
    13081 gtttgataac gaacgttttg accatctcta ccgcagcatt aatcgcatcg aagagaacgc
    13141 gaaagctgcc tgacatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc
    13201 gctggccttc accagcatgt tcagcctcag caccctgtta agcccggcac acgccgaaga
    13261 gcaggaaaag gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa acctgaaacc
    13321 gcaatggacg ccattcttac aggatatgga gaagaagctg ggcgtgaagg tgaacgcctt
    13381 ctttgcccca gactacgcag gcattatcca gggaatgcgc ttcaataaag tggatatcgc
    13441 ctggtacggc aacctgtcgg caatggaagc ggtggatcgc gccaacggcc aggtcttcgc
    13501 ccagacggtc gcggcggatg gatcgccagg ttactggagc gtgttgatcg tcaacaaaga
    13561 tagtccgatc aacaacctga acgatctgct ggcgaagcgg aaagatctca ccttcggcaa
    13621 tggcgatcct aactccacct ctggcttcct cgtccccggt tactacgtct ttgccaaaaa
    13681 caatatctcc gccagcgact ttaagcgcac cgtcaacgct gggcatgaaa ccaacgcgct
    13741 ggccgtcgcc aacaagcagg tggatgttgc caccaacaac accgaaaacc tcgacaagct
    13801 gaaaacctcc gcgccagaga agctgaaaga actgaaggtg atctggaagt cgccgctgat
    13861 cccaggcgat ccgatcgtct ggcgtaaaaa cctttccgaa accaccaaag acaagatcta
    13921 cgacttcttt atgaactacg gcaaaacgcc ggaagaaaaa gcggtgctgg aacgcctggg
    13981 ctgggcgccg ttccgcgctt ccagcgacct gcaactggtg ccgattcgcc agctcgcgct
    14041 gtttaaagag atgcagggcg tgaaaagcaa taaaggactg aatgagcagg acaagctggc
    14101 aaaaaccacc gagattcagg cgcagctgga tgacctggac cgcctgaaca acgcgttaag
    14161 cgcgatgagt tcggtgagta aagcggtgca gtaaatcgta ggtcggataa gacgccccgg
    14221 cgtcgcatcc gacaatgtgc aggcgttgat gccggatgcg gtgcaagcac cttatccggc
    14281 ctacagaccg gagccaaaca tgcaaaccat caccatcgcc ccacccaagc gcagctggtt
    14341 ctcgcttctg agctgggccg ttgttctcgc cgtgttggtc gtctcgtggc agggcgcgga
    14401 aatggccccg ctcacgctga ttaaagacgg cggcaacatg gcgacgttcg ccgccgactt
    14461 cttcccgccc gatttcagcc agtggcagga ttacctcacc gaaatggccg tcacgctgca
    14521 aatcgccgtc tggggcacgg cgctggcggt ggttctctcc atccccttcg gcctgatgag
    14581 cgccgaaaac ctggtgccgt ggtgggttta ccagcccgtt cgccgcctga tggacgcctg
    14641 ccgcgccatt aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg gcctcggccc
    14701 gttcgctggc gtgctggcgc tgtttatcca caccaccggc gtgctctcca aactgctttc
    14761 cgaagcggtg gaagcgattg aacccggccc ggtggaaggc attcgcgcca ccggcgccaa
    14821 caagctcgaa gagatcctct acggcgtgct gccgcaggtg atgccgctgc tgatctccta
    14881 ctccctctat cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca tggtcggtgc
    14941 aggcgggatc ggcgtcaccc tgtgggaagc gattcgcggt ttccagttcc aacaaacctg
    15001 cgccctgatg gtgcttatca tcgtcacggt cagcctgctg gatttcctct ctcaacggtt
    15061 gcgtaagcac tttatctgat aagcgaggca ttgatatcta tgcacttgtc tacacatccg
    15121 accagctacc caacacgcta tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa
    15181 cactaccgct gcggcgacta tcttcccgcc gagcagcaac tggcggcgcg ctttgaggtg
    15241 aaccgccaca ccctgcgccg cgccatcgac caactggtgg aaaaaggctg ggtacagcgc
    15301 cgtcagggcg tcggcgtgct ggtgctgatg cgcccgttcg attacccgct caacgcccag
    15361 gcgcgtttta gccagaacct gctggaccag ggtagccatc ccaccagcga aaaactgctg
    15421 tcggtattgc gccccgcgtc cggtcacgtc gctgacgcgc tggggattac cgagggcgag
    15481 aacgttatcc atttgcgcac cctgcgtcgc gtcaacggcg tcgcgctctg tttaatcgac
    15541 cactactttg cggacctcac cctttggccg acgctgcaac gcttcgacag cggttcgctg
    15601 cacgattttc tgcgcgagca aaccggaatt gcgctgcgcc gcagccagac gcggatcagc
    15661 gcccgtcgcg cccaggccaa agagtgccag cgtcttgaaa ttccgaatat gtcgccgctg
    15721 ctgtgcgtgc gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc
    15781 agcctgacgc gcgccgacat gattgaattc actatggagc actgaatgca cgcagatacc
    15841 gcgacccgcc agcactggat gtccgtactg gcgcacagcc aaccggctga actggcagcc
    15901 cgcctgaaag cactgaacat caccgccgac tatgaggtta tccgcgccgc cgaaaccggc
    15961 ctggtgcaga ttcaggcgcg gatgggcggc accggcgaac gcttttttgc cggcgacgcc
    16021 acgctcaccc gcgccgccgt acgcctgact gacggcacgc tcggctacag ctgggtgctg
    16081 gggcgcgaca aacagcacgc cgaacgctgc gcgctgattg acgcgctgat gcagcaatct
    16141 cgctattttc aaaacttatc agaaaccctt attgccccgc tggacgctga ccgtatggca
    16201 cgcattgccg cacgccaggc cgaagtgaac gccagccggg tcgacttctt tacgatggtt
    16261 cgcggagaca acgcatgacc ctggaaaccg cttttatgct tcccgtgcag gatgcccagc
    16321 acagttttcg tcgcctgtta aaggccatga gcgagccggg cgtgattgtc gccctgcatc
    16381 agctcaagcg cggctggcag ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg
    16441 ccgataacga cacgccggtg tggcttgccg cgccattgag taacgatatc gtcagccaga
    16501 gcctgcgttt tcataccaac gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg
    16561 tgacggatga ggcgatttcc agcgaacagc ttaacgccct ttccaccggc accgccgttg
    16621 cgccggaagc gggtgcaacg ctgattttac aggtcgccag cctgagcggc gggcgcatgt
    16681 tacgtctcac cggcgcgggc attgccgaag aacgaatgat cgctccgcag ctgccggagt
    16741 gcattctgca cgaactcacc gagcgcccgc acccgttccc gctcggcatc gacctgatcc
    16801 tgacctgcgg cgagcgcctg ctggctattc cgcgaaccac tcatgtggag gtgtgctgat
    16861 gtacgttgcc gtgaaagggg gcgagaaggc gatcgacgcc gctcacgccc tgcaagagag
    16921 ccgacgccgg ggcgataccg atttgcctga actgagcgtc gcccagattg aacagcagct
    16981 taatcttgcg gtggatcgcg tgatgaccga aggcggcatt gccgaccgcg aactggcggc
    17041 gctggcgctg aaacaggcca gcggcgataa cgtcgaagca attttcctgc tgcgcgccta
    17101 ccgcaccacg ctggcgaagc tcgcggtaag cgagccgctc gacaccacag agatgcgtct
    17161 cgaacggcgt atttcggcgg tttataaaga cattcccggc ggccagctgc ttggcccaac
    17221 ctacgactac acccatcgcc tgctcgattt caccctgctg gcaaacggtg aagcgccgac
    17281 gctgaccacc gccgacagcg aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg
    17341 tcaggggctg gcgaagtttg aagaggatag cggcgcacag ccggatgata tcacccgcac
    17401 gccgccggtt tacccctgct cacgttcttc ccgtttgcag cagttgatgc gcggcgacga
    17461 aggctatttg ctggcgctgg cctactccac ccaacgcggt tacgggcgca atcacccgtt
    17521 cgcgggcgag atccgcagtg gttacatcga cgtgtcgatt gtgccggaag agctgggatt
    17581 tgcggtgaac gtcggcgaac tgctgatgac cgaatgtgaa atggtcaacg gttttatcga
    17641 cccgccggat gagccgccgc acttcacgcg cggctacggg ctggtattcg gcatgagcga
    17701 gcgcaaagcg atggcaatgg cgctggttga ccgtgcgttg caagccccgg aatacggcga
    17761 gcacgcgaca ggcccggcgc aggatgaaga gttcgtgctg gcgcatgccg acaacgtcga
    17821 agccgcaggc tttgtctcgc acctcaaact cccgcactac gtcgatttcc aggccgaact
    17881 ggagctactc aaacgtctgc aacaggagca gaaccatggc taatctgagc ggctacaact
    17941 ttgcctacct cgacgagcag accaaacgca tgatccgccg cgccatctta aaagcggtgg
    18001 ctatccccgg ttatcaggtg ccgtttggcg ggcgcgagat gccgatgccc tacggctggg
    18061 gaaccggcgg cattcagctc accgccagcg tgattggcga aagcgacgtg ttgaaggtga
    18121 ttgaccaggg tgcggatgac accaccaacg ccgtgtcgat tcgcaacttc tttaagcgcg
    18181 tgaccggggt aaacaccact gaacgtacgg acgatgcgac gcttatccag acgcgtcacc
    18241 gcatccccga aacgccgctg accgaagatc agatcattat cttccaggtg ccaatcccgg
    18301 aaccgctgcg ctttatcgag ccgcgcgaaa cggagacccg cactatgcac gcgctggagg
    18361 agtacggcgt gatgcaggtg aaactgtatg aagatatcgc ccgcttcggt catatcgcca
    18421 ccacctatgc ttatccggtg aaggtgaacg ggcgctacgt gatggacccg tcgccgatcc
    18481 cgaaattcga taacccgaaa atggacatga tgcccgccct gcagctgttc ggagcagggc
    18541 gcgagaagcg catctatgcg gtgccgccgt ttacccgcgt ggaaagtctc gatttcgacg
    18601 atcacccgtt caccgttcag cagtgggatg agccatgcgc catctgcgga tcgacccaca
    18661 gctatcttga tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc tgctccgata
    18721 ccgattattg ccgccaacag agcgaggcga aaagccaatg aataaaccgt tactttcggt
    18781 caataacctg acccaccttt acgcgccggg caaaggcttt agcgatgtct cttttgattt
    18841 atggccggga gaagtgctgg gcattgtcgg agaatccggc tccgggaaga ccacgctgct
    18901 gaagtcgatc tccgcgcgtc tgacgccgca gcagggggag attcactacg agaaccgttc
    18961 gctgtatgca atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtagt
    19021 gcatcagcat ccactcgacg gcctgcgccg tcaggtctcg gcaggcggca atatcggcga
    19081 gcggttgatg gcgaccgggg cgcgtcatta cggcgacatt cgcgccaccg cgcagaagtg
    19141 gctggaagag gtggagattc ccgccaaccg gatcgacgac ctgccgacca ccttttccgg
    19201 cggtatgcag cagcgtttgc agattgcccg caacctggtg acgcatccga agctggtgtt
    19261 tatggatgaa ccgaccggcg ggctggatgt gtcggtgcag gcccgcctgc tcgacctgct
    19321 gcgcggcctg gtggtggagc tgaacctcgc ggtggtgatt gtcacccatg atttaggcgt
    19381 cgcccgcctg ctggcggacc gtttgctggt gatgaagcag gggcaagtgg tggagagtgg
    19441 gttaaccgac cgcgtgctcg acgacccgca tcatccgtat acacagctgc tggtgtcatc
    19501 ggttttgcag aattgagccg gtgccggatg cggcgtaaac gccttatccg gcctacaaat
    19561 gcgctccccg taggtcggat aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg
    19621 cgaaaaatga ttaacgtaca aaacgtcagt aaaaccttca tcctgcacca gcaaaacggc
    19681 gtgcgcctgc ccgtcctcaa tcgcgcctcg ctcaccgtca acgcgggcga atgcgtggtg
    19741 ctccacggcc attccggcag cggcaaatca actctgctac gctcgctgta cgccaactat
    19801 ctacccgacg aaggtcaaat ccagatcaaa cacggtgacg agtgggtaga cctggtcacc
    19861 gcgccagcgc gcaaagtggt ggaaatccgc aaaaccaccg tcggctgggt gagccagttt
    19921 ctgcgcgtca tcccgcgtat ctcagcactg gaagtggtga tgcagccgct gctcgatacc
    19981 ggcgttccgc gtgaagcctg cgccgctaaa gccgcgcgtc ttctcacccg cctgaacgtg
    20041 ccggaacgcc tgtggcacct ggcaccatcg acattttccg gtggcgaaca gcagcgcgtc
    20101 aacatcgccc gcggctttat cgtcgactac cccattctgc tgcttgacga acctaccgcc
    20161 tcgctggacg ccaaaaacag cgccgcggtg gtggaactga ttcgcgaagc caaaacccgt
    20221 ggcgcagcca tcgtaggcat cttccatgac gaagctgtac gtaatgacgt cgccgaccgc
    20281 ctgcacccaa tgggagcctc ttcatgatta tcaataacgt taagctggtg ctggaaaacg
    20341 aggtggtgca cggttcgctg gaggtgcagg atggcgaaat ccgcgccttt gccgaaagcc
    20401 agagccgcct gccagaagcg atggacggcg aaggtggctg gctactaccc ggcctgattg
    20461 agctgcatac cgacaacctc gataaattct tcaccccgcg cccgaaagtc gactggcccg
    20521 cccattcggc gatgagcagc cacgacgcgc tgatggtggc gagcggcatc accaccgtac
    20581 tggatgccgt agcaattggc gacgtgcgcg acggcggcga tcggctggaa aatctggaga
    20641 agatgatcaa cgccatcgaa gagacgcaga aacgcggcgt caaccgcgcc gagcaccgcc
    20701 tgcatctgcg ctgcgaactg ccgcatcaca ctacgctgcc gctgtttgaa aaactggtgc
    20761 agcgcgagcc ggtgacgctg gtgtcgctga tggatcactc gccgggccag cgccagttcg
    20821 ccaaccgcga gaagtatcgc gagtattatc agggcaaata ctcgctcacc gatgcgcaga
    20881 tgcagcagta cgaagaagag caactggcgc tcgccgcacg ctggtcgcag ccgaatcgcg
    20941 aaactatcgc cgccctgtgc cgcgtaagac aaattgccct cgccagccac gatgacgcca
    21001 cgcacgccca cgttgccgaa tcccaccagc ttggcagcgt gatcgccgaa tttcccacta
    21061 cgttcgaagc agcggaagcc tcgcgcaagc atggcatgaa cgtgctgatg ggcgcgccaa
    21121 atatcgtgcg tggcggttcg cactccggca acgtggcggc cagtgaactg gcgcagcttg
    21181 gcctgctgga tatcctctct tccgactact accccgccag cctgctggat gcggcgttcc
    21241 gcgtcgccga tgacgagagc aaccgcttta cgctgccgca ggcggtgaag ctggtgacca
    21301 aaaatccggc gcaagcgctt aatctccagg atcgcggggt gattggcgag ggtaaacgcg
    21361 ccgacctggt gctggcgcat cgcaagggca accacattca tatcgaccac gtctggcgtc
    21421 agggtaaaag ggtgttctga tgatgggaaa actgatttgg ttaatggggc cgtccggctc
    21481 cgggaaagat agcctgctgg cggaactccg cctgcgggaa caaactcagc tactggtggc
    21541 gcatcgctac atcacgcgcg acgccagcgc tggaagcgaa aaccatatcg ccctgagcga
    21601 gcaggagttt tttacccgcg cggggcaaaa tctgctggcc ttaagctggc acgctaacgg
    21661 tctgtattat ggcgtcggcg tcgagattga cctctggctg cacgctggat tcgacgtgct
    21721 ggtcaacggc tcacgcgccc atctgccgca ggcgcgggcg cgctatcaat cggcgctgct
    21781 gcccatttgc ttacaggttt cgccagagat cctgcgccag cgcctggaaa accgtggccg
    21841 cgaaaacgcc agtgaaatta acgcccggct ggcgcgcgcc gcccgctata ctccacagga
    21901 ttgccatacg ctcaataatg acggcagcct gcgccagtcg gtcgacacgc tgctgacgct
    21961 aatccatcag aaggagaaac accatgcctg cttgtgagct tcgcccggcc acgcagtacg
    22021 acaccgacgc ggtttacgcg ttgatttgcg agctaaaaca ggcggagttt gaccaccacg
    22081 cgtttcgcgt gggctttaac gccaacctgc gcgacccgaa catgcgctac catctggcgc
    22141 tgctggatgg cgaagttgtc ggtatgatcg gcctgcattt gcagtttcat ctgcatcatg
    22201 tcaactggat cggcgaaatt caggagttgg tggtaatgcc gcaggcgcgc ggtctgaacg
    22261 tcggcagtaa gttactggcg tgggcagaag aagaagcccg ccaggccggg gccgaaatga
    22321 ccgaactttc gaccaacgtg aagcgccacg acgcgcaccg tttctatctg cgagaaggct
    22381 acgagcagag tcacttccgc ttcaccaagg cgctgtaaca tgagtctgac cctcacgctc
    22441 accggcaccg gcggcgcaca gggcgttccg gcatggggct gcgagtgcgc ggcctgcgac
    22501 cgagcgcggc gctcaccgca gtatcgccgc cagccatgca gcggcgtagt gaagtttaac
    22561 gacgcaatca ccctgatcga cgccgggctg cacgatctcg ccgatcgctg gtcgcccgga
    22621 tcgttccagc agtttttgct gacgcattat catatggatc acgtccaggg gctgtttccg
    22681 ctgcgctggg gcgttggcga tccgatcccg gtttacggcc cgccggatga ccagggttgc
    22741 gacgatctgt ttaaacatcc gggcctgctt gatttcagcc acacagtgga accgtttgtg
    22801 gtatttgatt tgcaggggtt gcaggtcacg ccgctgccgc ttaaccactc aaaactgacc
    22861 ttcggttatc tgctggaaac ggcacatagc cgggtggcgt ggctatctga caccgctggc
    22921 ctgccggaaa aaacacagaa atttttactc aataatcatc cgcaggtgat ggtgattgat
    22981 tgcagccacc cgccgcgcga ggatgcgccg cgtaatcact gtgatttaaa taccgtgctt
    23041 gcgctcaatc aggttatcca ctcgccacgg gtgattctga cccatatcag ccaccagttt
    23101 gatgcgtggc tgatggagaa tgcgctgccg ccgggttttg aggcggggtt tgatgggatg
    23161 gagattgggg tggcgtgatg agagggaatg tgcgcgctgg ccccctcacc ctaaccctct
    23221 ccccagaggg gcgaggggac cgattgtgct cgatattgaa tattgcgctg gttttctccc
    23281 tctccccatt ggggtgaggg gcgatgcctg ctccataccc aacctcatcg cccatactca
    23341 tcttccattc tccgctcttc atcttctagc tgccgacgct cctgatcaag ctggcgctgg
    23401 cgatcgtcca gctgcctgcg gcgatcttca aactggcggc ggcggtcgtc atattgtctg
    23461 cgccgatcgt cgctcacttc acgctgccag ccgtcgtcgc gcgaatcttc atagtctcgc
    23521 ccacggtcag ggttataagc gtcgttaatc gcctgctgaa tattgccaat ggtgtcgtcg
    23581 ataatatcgg cctgggccgg aacggtggac agcgtgagca gggtgaataa aagaaatagc
    23641 ggaaagcgtt tcattagcca acctcaaaaa gaaactctat ccacattagt cattactcat
    23701 ccatgcaagt agtggatgaa tctcaatttc tccactgctc tattgccgta atcgcctcca
    23761 cgcgttgttg atgacgaccg ccttcgtact gtgcgcccag ccacgcatcc acaatcattt
    23821 ttgccagttc gaggccaacc actcgtgaac caaaagccag cacgttggtg tcgttatgct
    23881 gccgcgaaag ttgcgcggaa taaggttcgc tacagacgac cgcgcgaatt ccggcaaact
    23941 tgttcgccgc tatcgaaata ccgacgccag taccacaaat caaaatcccg ccatcaacct
    24001 ctccgccagc aacagccagt gcgacttgac tggcgtaatg tggataatca gtacgctctg
    24061 acgaccaggt tcctttatca atcacttcaa cgccacgctc aactaaatgt gccactattt
    24121 catgttttaa aatgaaaccg acatgatcac agccaaatgc aatctttttc attcatcttc
    24181 cccgtccgtg caaaacttca caatcaaaaa acatttgtga tgttaatgaa ttaaaaacac
    24241 ccaaaatcga tgaattacgc cttcctgatc tgttttacag agttacatct gacagcgcaa
    24301 tagcgttaaa gacactcacc atccagcaac atctatcatc taaaaaacca gaaaaacaaa
    24361 taacatcatg tttttaaact aattaaatga aataaaattt taagccactc gccattgttc
    24421 acaataaaat aaactttata aattttattt ttttgtgaag tcgccagcat cttttctgtt
    24481 cttgctgtgg tgatatagtg gcgtcttcaa ttcaaggaca agagaacgtg atgagccagt
    24541 cagagtttga ttcagcgctt ccgaacggta tagggttagc gccttacctg cgaatgaagc
    24601 aggaaggaat gacagaaaat gaaagccgca tcgtagagtg gttactcaaa cccggtaacc
    24661 tgagttgtgc acccgcaatt aaagatgtcg cagaagctct gtcggtatct gaagcgatga
    24721 tagttaaggt atcaaagctg ctggggttta gcggctttcg taacttacgc agtgcgctgg
    24781 aagattattt ttctcagtca gaacaggtat tgccttccga gttggctttt gatgaagcgc
    24841 cgcaggatgt ggtgaataag gtatttaaca tcactttacg caccattatg gaaggtcagt
    24901 cgatcgtcaa cgttgatgag atccaccgtg ccgcccgctt tttctatcag gccagacagc
    24961 gggatttgta cggtgccgga ggatcaaatg ctatctgtgc tgatgtacag cacaagttct
    25021 tgcgcattgg cgtacgctgt caggcctatc ctgatgctca catcatgatg atgtccgctt
    25081 cgttgttaca ggaaggagat gttgtgctgg tagtgaccca ttccgggcga accagtgatg
    25141 taaaagcggc cgtagaactg gcaaaaaaga acggggcaaa gattatttgt ataacccata
    25201 gctaccattc accgatagcg aaactggccg attatattat ttgctcacca gccccggaaa
    25261 cgccgttatt aggtcgtaat gcctcggcaa gaatattaca actaactttg ctggacgctt
    25321 tttttgtctc tgtcgcccag ctcaacattg aacaagctaa tattaatatg caaaaaaccg
    25381 gcgcaattgt tgatttcttc tcaccaggcg cgctgaaata agaaaaaggt aattccgcca
    25441 tcgcggagtt acctgtcccc ttaccgtga
//
LOCUS       DAZFWP020000068        25203 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFWP020000068 DAZFWP020000000
VERSION     DAZFWP020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.068"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(294..1886)
                     /gene="lsrK"
                     /locus_tag="ACHK0Z_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             complement(294..1886)
                     /gene="lsrK"
                     /locus_tag="ACHK0Z_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU4502558.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1965..2918)
                     /gene="lsrR"
                     /locus_tag="ACHK0Z_RS12790"
                     /old_locus_tag="pgaptmp_002558"
     CDS             complement(1965..2918)
                     /gene="lsrR"
                     /locus_tag="ACHK0Z_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU4502559.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3167..4702
                     /gene="lsrA"
                     /locus_tag="ACHK0Z_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             3167..4702
                     /gene="lsrA"
                     /locus_tag="ACHK0Z_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU4502560.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4696..5724
                     /gene="lsrC"
                     /locus_tag="ACHK0Z_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             4696..5724
                     /gene="lsrC"
                     /locus_tag="ACHK0Z_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU4502561.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5724..6716
                     /gene="lsrD"
                     /locus_tag="ACHK0Z_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             5724..6716
                     /gene="lsrD"
                     /locus_tag="ACHK0Z_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU4502562.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6728..7750
                     /gene="lsrB"
                     /locus_tag="ACHK0Z_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             6728..7750
                     /gene="lsrB"
                     /locus_tag="ACHK0Z_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU4502563.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7777..8652
                     /gene="lsrF"
                     /locus_tag="ACHK0Z_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             7777..8652
                     /gene="lsrF"
                     /locus_tag="ACHK0Z_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU4502564.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8676..8966
                     /gene="lsrG"
                     /locus_tag="ACHK0Z_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             8676..8966
                     /gene="lsrG"
                     /locus_tag="ACHK0Z_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU4502565.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9023..9781
                     /gene="tam"
                     /locus_tag="ACHK0Z_RS12825"
                     /old_locus_tag="pgaptmp_002565"
     CDS             9023..9781
                     /gene="tam"
                     /locus_tag="ACHK0Z_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU4502566.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9785..10699)
                     /locus_tag="ACHK0Z_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             complement(9785..10699)
                     /locus_tag="ACHK0Z_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU4502567.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLHFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10906..12414)
                     /gene="uxaB"
                     /locus_tag="ACHK0Z_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             complement(10906..12414)
                     /gene="uxaB"
                     /locus_tag="ACHK0Z_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU4502568.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLSIALNGMTKFRTRILP
                     QLLAGQKANGTLPARLTFALAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDR
                     VIGTQELVAIVLAEKDHWEQDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12641..14059)
                     /locus_tag="ACHK0Z_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             complement(12641..14059)
                     /locus_tag="ACHK0Z_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4502569.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14198..14557)
                     /locus_tag="ACHK0Z_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             complement(14198..14557)
                     /locus_tag="ACHK0Z_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU4502570.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14557..15483)
                     /gene="glsB"
                     /locus_tag="ACHK0Z_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     CDS             complement(14557..15483)
                     /gene="glsB"
                     /locus_tag="ACHK0Z_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU4502571.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15547..16935)
                     /gene="sad"
                     /locus_tag="ACHK0Z_RS12855"
                     /old_locus_tag="pgaptmp_002571"
     CDS             complement(15547..16935)
                     /gene="sad"
                     /locus_tag="ACHK0Z_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU4502572.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            17036..17917
                     /locus_tag="ACHK0Z_RS12860"
                     /old_locus_tag="pgaptmp_002572"
     CDS             17036..17917
                     /locus_tag="ACHK0Z_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4502573.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            17994..19108
                     /gene="yneK"
                     /locus_tag="ACHK0Z_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /pseudo
     CDS             17994..19108
                     /gene="yneK"
                     /locus_tag="ACHK0Z_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19258..20448
                     /gene="ydeA"
                     /locus_tag="ACHK0Z_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             19258..20448
                     /gene="ydeA"
                     /locus_tag="ACHK0Z_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU4502574.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20473..21138)
                     /gene="marC"
                     /locus_tag="ACHK0Z_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             complement(20473..21138)
                     /gene="marC"
                     /locus_tag="ACHK0Z_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU4502575.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21350..21784
                     /gene="marR"
                     /locus_tag="ACHK0Z_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             21350..21784
                     /gene="marR"
                     /locus_tag="ACHK0Z_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU4502576.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKKLTADEVATLEHLLKKVLP"
     gene            21804..22187
                     /gene="marA"
                     /locus_tag="ACHK0Z_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             21804..22187
                     /gene="marA"
                     /locus_tag="ACHK0Z_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU4502577.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22219..22437
                     /gene="marB"
                     /locus_tag="ACHK0Z_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             22219..22437
                     /gene="marB"
                     /locus_tag="ACHK0Z_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU4502578.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22494..23933)
                     /locus_tag="ACHK0Z_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             complement(22494..23933)
                     /locus_tag="ACHK0Z_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4502579.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNYEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHTRG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23958..>25203)
                     /locus_tag="ACHK0Z_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             complement(23958..>25203)
                     /locus_tag="ACHK0Z_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU4502580.1"
                     /translation="GFSYQGYFRSGWGTGNHGSPQTYAAGSLGRFGNEMSGWFDLTLN
                     QRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDSANENIMQFSDIYLTTRGFLPFAPEA
                     DFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIENWALGVGLFDMSLSRDDVDVYSRDF
                     SRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSAPNKTDQQQDNENDNSYFEMKDSWM
                     LTSVLRQNLQRDTFNEFTLQVANNSYASSFASFSDASNTMAHGRYYYGDHTNGIAWRL
                     ISQGEMYLTDNIIMANALVYSHGEDVYSYESGAHSDFDSIRTVIRPAWIWNTWNQTGL
                     ELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGESILGSRPEIRFYGTYINILDNELS
                     NFKFNENSKDEFMAGIQAEVWW"
BASE COUNT         6455 a         6360 c         6040 g         6348 t
ORIGIN      
        1 ataggaaaat tatcatcatc atatttaaca aagaatagca ctaattgcta aaaatcgaag
       61 tttattaaac cccttttgca tccattaatg tctttaggaa tattcgctat aaaataaggg
      121 ttatgcttaa tgctttaaga aaaatagcaa ttttcccttg aatatagtac tggtgatgga
      181 acgatgaatc tgcaagctgg catttaacaa gccagctcta aaagaaggga aataagaaaa
      241 actatactca aaaactaaca gccacggtca tcatgatgtg gctgtcaatg aaactataac
      301 ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa ccagccccag ctgatcctga
      361 taaactgcct gccacttatc gcgtgaatcc tgataaagtt catgcttttc cgggtctggt
      421 gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt ctgccattga tgaaaaaatt
      481 ccggcaccga cgccagctgc aatggcacat cctaatgcgg tggcttcttt gactaccgga
      541 atattgacgg gtaatcccga gacatcagcg agaatttgac tccataattt cccttttgaa
      601 cctccgccag caaaaaccag cgaggtggga tgaatattcg agaaatcagc aatttgctgc
      661 aagttacacg ctgatacaat cgccgcattt tcttccagcg cacggaacaa tgtcgctttg
      721 ttacatttat ccgggtcaat ggacaagtta ataaaggaag gcgcagcgtg ataccaggtt
      781 ttaaagcgca ttctgtcgga gaagatcggc attacgcccc acgacccagg cggcacccga
      841 ctggccatct cttccagcag cgtataggtg tcgatgccta aacgttcggc aatcagtttt
      901 tcttcggcac agaaagcatc gcggaaccag cgcatggtga gtccggtaaa aaagcttata
      961 gattcagctt gtaccatgcc aggaataaca tgaggattaa cgcgcacgtt catttctggg
     1021 tctgtcaccg gcgcggctaa atttacaact tgctgccaga atgtgccgcc aagaaccgcg
     1081 gtttgtgccg gacgcacaac gcctaaccca aggcaaccaa gctgcacgtc gcctcctcca
     1141 acgaccaccg gagtgcccgc cttcagaccg cagagttccg ccgcttgtga acttaccacg
     1201 cccagcaatg tgccggtttc tttgacagga gaaagaatat cggcacgcag gccagccata
     1261 tccagcaatg caggtttcca gtcacgggtg gttagatcaa gaagtcccgt ggtgccagcg
     1321 ttagagggat ccaccgccag ttcgccgctg agcatatagg ccagccagtc gctgatcatg
     1381 gtgatggttg atgcctgacg gtaaatatcg gaacgatggt gcgccagcca aagtaatctg
     1441 gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat aaacttcgtt ttcaaaggta
     1501 ttgttgtgca gttctttaag ttcgctaact tcgcgtgccg ctctggcatc cacattggcg
     1561 caggcccaga tcggggctcc ttcattatta tataaaacaa tgccttcacg catcgaacat
     1621 gccgaaacgg cagcgatata ctccggggct atgccggcgt tgtgcagcgc ctggcgcata
     1681 cactcacacg ccagttgcca gtttttgttg agatcaaatt ccatagaacc aggaacgtcc
     1741 ggtactgcca ggtgccgcca ctccgcctgt cccactgcta tttgattgcc ttccaggtcg
     1801 aatatcacag cccgaatact tccggtacct gcatccagcg ccatcaggta atactttgat
     1861 tctgaagggg taaagagtcg agccattaga ttatcctcgg cttatgcgtt ctgtctgttc
     1921 ctctatacgt tctccatcat tcccggtaat aaggtcatgc aaatttaact acgtaaaatc
     1981 gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat aaccgccttt cattgcagcg
     2041 gcaattgctt cggctttatt ttctccccct gccacgccaa cccggacggg tatggtcttc
     2101 agcgagctta aaggtaagcc aatcagttcg ttatgtattt taatatccgt gacaacgtca
     2161 ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg ccccttttcg accaatcatt
     2221 aactgttcgc cctggctgat ataaccggag cgaatgatcg tcgcgtcgtc ctgttgactg
     2281 acggccccaa tgccgacaat cgccacatcg gctgcttgcg cggctaacag aacatctttg
     2341 acgcaatttt cattttttag cgtacgggca atgtcagcgg aggatgcccg caacggagcc
     2401 ggaataatat tcacactgca cgccgcgtta agctgcccga tacccgtcat ataagaaccg
     2461 acgccaccgg agagcgtgac caggcgaatt tgctgtgacg aaataaaacc acttaagcgt
     2521 tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca gcatctgttg tggttgaagt
     2581 aaactcatca acatatgcgc cgcgcctatc cccagtcgcc cgccgacatc agcatccgca
     2641 agcccaggga tcacccggac atgttgcagc gaaaactgac gacgtaattg agtttcatat
     2701 tccagacagc cttcaaagcg agaattaatc tgtacgcgaa taatgccgga ctgatgccct
     2761 ttctccagca agcgcgacac tttcaaacgg gtcaggccga gacgatcgct gatctcgctc
     2821 tgggtcagcc cgtcgtgata gtaaaaccac gcgatccgcg cgacctgttc ttcttcacac
     2881 attccctgtt ctgaaattgc cgaatcgttg attgtcataa ttcattcttc actttgaaca
     2941 tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt tatatctgtg atggcagcca
     3001 cagtttgact ctacgagcat gaacaaacgc aaccgtgaaa atcaaaatag cataaattgt
     3061 gatctattcg tcggaaatat gtgcaatgtc cacctaaggt tatgaacaaa ttaaaagcag
     3121 aaatacattt gttcaaaact cacctgcaaa actgaacggg ggaaatatgc aaacgagtga
     3181 tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat aaacagtatt caggggtcaa
     3241 tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag gtccacgccc tgctcggcgg
     3301 caatggtgcc ggtaaatcga cgttaatgaa gattattgcc ggtattaccc ctgctgatag
     3361 cggtacgctg gagattgggg gcaacaacta cgccagatta acgccagttc atgctcatca
     3421 gctgggcatt tatctcgttc cccaggaacc gctgcttttc ccaagcctgt cgataaaaga
     3481 aaacatcctg tttgggatgg caaaaaaaca gctctccatg caaaaaatga agaacttgct
     3541 ggcggcgctg ggctgccagt ttgatctgca tagtctggca ggatcgctgg atgtcgccga
     3601 tcgccaaatg gtggaaatcc tccgcgggct aatgcgcgac tcgcggattc tgatcctcga
     3661 tgaacctacc gcctcgctta cccctgcgga aaccgaacgc ttgtttactc gcttgcaaga
     3721 gctgcttgct actggcgtgg gtattgtttt tatctcgcat aagctgccgg aaattcgcca
     3781 gattgccgat cgaattagcg tgatgcgcga cggaaccatc gccttaagcg gcaaaaccag
     3841 cgaactgtct accgacgaca ttattcaggc catcacccca gcggtacggg aaaaatcgct
     3901 ctctgccagc caaaaattat ggctggagtt acctggtaac cgcccacaac atgctgccgg
     3961 aacgccggtg ctgacactgg aaaacctgac cggcgaaggt ttcaggaatg tcagcctgac
     4021 gctcaatgcc ggagaaattc tgggcctggc tgggctggtg ggggccggac gcacagaact
     4081 ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga cgcattatgc tgaatggtaa
     4141 agagatcaat aaattatcca ctggagaacg tttactgcgc ggtctggttt atctgccgga
     4201 agatcgccag tcatccggac tgaatctcga tgcttcgctg gcctggaacg tctgcgccct
     4261 tactcataac cttcgtggat tctgggcgaa aaccgcgaaa gataatgcca ccctggaacg
     4321 ttatcgtcgg gcgctgaata ttaaatttaa ccaaccggaa caagctgcac ggacattatc
     4381 cggtggcaac cagcaaaaaa tcctcattgc caaatgcctg gaagcttcgc cgcaagtatt
     4441 gattgtcgat gagccgacgc gcggcgtgga tgtctcggcc cgtaatgata tctaccagct
     4501 gttgcgcagc atcgccgcac aaaatgtggc agtgctgctt atctcctccg atctggaaga
     4561 gatcgaactg atggcagatc gcgtgtatgt gatgcatcag ggcgaaattg cccactctgc
     4621 actgaccggg cgcgatatta atgtcgagac tattatgcgc gttgccttcg gcgatagtca
     4681 gcgtcaggag gtgtcatgct gaagtttatt cagaacaacc gtgaaatcac ggcactgctg
     4741 gcggtggtgc tgctgtttgc attacccggt tttctcgacc gccagtattt aagtgtgcaa
     4801 acgctgacca tggtttatag cagcgcgcaa atcctgatcc tgctggcaat gggcgcgacg
     4861 ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt cgattaccgg aatgtgcgca
     4921 gtgctgttgg ggatgttact gaacgcgggg tattcactac ctgttgcttg tgtcgcgact
     4981 ttactgcttg gtttgctcgc gggatttttc aacggtgtcc tggtcgcgtg gctaaaaatc
     5041 cctgccattg ttgccaccct tggcacgtta gggttgtaca gaggcatcat gttgctgtgg
     5101 actggcggta aatggattga agggttaccc gccgaactga aacagctctc cgcaccgctg
     5161 ctgtttggcg tttccgcgat tggttggttg acgataattc tggtggcatt tatggcctgg
     5221 ctgctggcaa agacggcgtt tggacgcagt ttttatgcca cgggcgataa tttacagggc
     5281 gctcgtcaac tgggtgttcg tacggaagcc attcgcattg tggcattttc gttgaacggc
     5341 tgcatggcgg cactggcggg aattgtgttt gcttcgcaga ttggttttat ccccaaccag
     5401 accggtaccg ggctggagat gaaagcaatt gcagcctgcg tgctgggcgg cattagtttg
     5461 ctcggcggtt ccggtgcgat catcggtgcg gtactcggcg catggttcct gacgcagatc
     5521 gatagcgtac tggtgctgtt gcgcattccg gcatggtgga acgattttat cgcgggcctc
     5581 gtgctgctgg cggtgctggt gtttgatgga cgcctgcgtt gtgcgctgga acgtaatcta
     5641 cggcggcaaa aatatgcccg ctttatgacg ccaccgccat ccgttaaacc cgcttcgtca
     5701 ggtaaaaaac gggaggccgc ataatgcgta ttcgctacgg ttgggaactg gctcttgccg
     5761 cactgctcgt tattgagatt gtcgcatttg gtgcaattaa cccgcgaatg ttagatctca
     5821 atatgttgct gttcagcacc agtgacttta tctgcattgg cattgtcgcc ctaccgctga
     5881 cgatggtgat tgtcagtggc gggatcgata tttcgtttgg ttcgaccatc ggcctctgcg
     5941 ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat gccgctggcg attctcctga
     6001 ccttactgct cggcgcattg tgcgggctga tcaacgccgg attaattatc tataccaaag
     6061 ttaacccgct ggtgattacg cttggcacgc tgtatctgtt tgccggaagc gccctactgc
     6121 tttccgggat ggccggagcg acggggtacg aaggtattgg tggattcccg atggcgttta
     6181 cagatttcgc taacctggat gtgctgggac tccccgttcc gctgattatc ttcctgatat
     6241 gtctcctcgt tttctggctc tggctgcata aaacccatgc cggacgtaat gtgtttttga
     6301 ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc agttaaccgc accttatgtg
     6361 cgctctatgc catgacgggg ctggcgtctg cggtcgccgc tgtgctgctg gtgtcgtatt
     6421 ttggttcagc acgttccgat ctcggtgcgt cgtttctgat gcccgccatc accgccgtgg
     6481 tgcttggcgg tgccaatatt tatggtggtt ccggttccat tatcggcacc gccattgcgg
     6541 ttttattagt gggatatttg caacaaggtt tgcaaatggc aggagtgcca aatcaggtgt
     6601 ccagcgccct ttccggtgcg ctacttatcg tcgttgtcgt aggtcgttcc gttagcctgc
     6661 atcgccagca aattaaagag tggctggcgc gtcgggccaa taacccattg ccataaagga
     6721 tatcttcatg acacttcatc gctttaagaa aatcgcctta cttagcgctc ttggcattgc
     6781 cgcaatctct atgaatgtgc aggccgcaga gcgtattgca tttattccca aactggttgg
     6841 cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa gcgggtaaag agctgagcgt
     6901 tgatgtgacc tacgacgggc cgacagaacc cagtgtttct ggtcaggtac agttgattaa
     6961 taacttcgtc aatcaaggtt ataacgccat tatcgtttct gcggtttcgc ctgatggctt
     7021 gtgtccggca ctgaaacgcg ccatgcaacg tggtgtgaga gtgctgacct gggactctga
     7081 tactaaaccg gagtgccgct cttactacat taatcaggga acgcccgccc agttgggagg
     7141 tatgttggtg gatatggcgg cgcgtcaggt gaataaagac aaagccaaag tcgcgttttt
     7201 ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg aaagaagcga aagcgaaaat
     7261 cgccaaagag catcctggct gggaaattgt cactacgcag tttggctata acgatgccac
     7321 taaatcatta caaaccgcag aaggaatatt aaaagcgtat agcgatctcg acgccattat
     7381 cgcccccgac gccaacgccc tgcccgctgc cgcacaagcc gcagaaaacc tgaaaaatga
     7441 caaagttgcg attgtcggat tcagtacgcc aaacgtgatg cgtccatatg tggaacgcgg
     7501 cacggtgaaa gaatttggcc tgtgggatgt ggttcagcaa ggcaaaattt cagtgtatgt
     7561 cgcggatgca ttattgaaaa aaggatcaat gaaaacgggc gacaagctgg atatccaggg
     7621 cgtaggtcag gtggaagtct cgccaaacag cgttcagggc tatgactacg aagcggatgg
     7681 taatggcatc gtactgttac cggagcgcgt gatattcaac aaagagaata tcggcaaata
     7741 cgatttctga tgtgcattac ttaaccggag taagttatgg cagatttaga cgatattaaa
     7801 gatggtaaag attttcgtac cgatcaaccg caacaaaata tcccttttat cctgaaaggt
     7861 tgcggtgcgc tggattgggg aatgcagtca cgcttatcgc ggatatttaa tccgaaaacg
     7921 ggtaaaaccg tgatgctggc ctttgaccat ggttattttc aggggccgac taccggactt
     7981 gaacgcattg atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg
     8041 cgcggcattt tgcgcagcgt agttccccca gcgaccaata agccggtggt actgcgggcg
     8101 tcaggtgcga actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat
     8161 gatgccgtgc gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagtgaatat
     8221 gaacatcagt cgatcaaaaa tattattcag ctggttgatg ccggaatgaa agtgggaatg
     8281 ccgaccatgg ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg ttatttctcg
     8341 ctcgcgactc gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta ttatgtcgaa
     8401 aaaggttttg aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa
     8461 aaattaccgg agcgcgaggc gctggaaatg tgctggcagg ctatcgatca gggcgcttct
     8521 ggtgtggata tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc
     8581 gtacaggcgg tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt
     8641 gaaaaacagt aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta
     8701 acgttcatga agacaagatt gacgagttta tcgaagtttt tcgccagaac cacctgggct
     8761 ctgtacagga agaaggcaat ttgcgcttcg atgtcttaca ggacccagaa gtgaattcgc
     8821 gcttttatat ctacgaagcc tataaagatg aagacgcagt ggcattccat aaaaccacgc
     8881 cccactacaa aacctgtgtc gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac
     8941 gtctgttcaa tgggttgatg ccgtgaggcg aattcatcag ttttatctac aattggggta
     9001 acgcgctgac gggagtaaat aaatgtctga ctggaacccc tctttatatc tgcactttgc
     9061 cgctgaacga tcgcgtccgg cggtggagct gcttgccaga gtaccgctgg agaatatcga
     9121 atacgttgcc gatcttggct gtggcccagg taacagcacc gcccttctaa accaacgttg
     9181 gcctgctgcc aggataacag gcatcgactc gtctccggcg atgattgctg aagcgcgcag
     9241 tgctttgcca gactgccagt tcgtggaagc agacatccgc aactggcaac cggaacaggc
     9301 actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact acgaattgtt
     9361 tcctcatctg gtttctttac ttaatccgca gggcgtgctg gcagtacaga tgccagataa
     9421 ctggctggag ccgacccatg tgctcatgcg cgaagttgcc tgggaacaaa actacccgga
     9481 tcgcgggcgt gagccgttgg ctggcgttca tgcttactac gatattttga gcgaagccgg
     9541 atgtgaggtc gatatctggc gaaccaccta ctatcaccag atgccgtcac accaggcgat
     9601 tatcgattgg gtgactgcca ctggattacg tccgtggtta caggatctga ccgagagcga
     9661 acagcagctt tttcttaagc gctaccatca gatgctggaa gagcagtatc cactgcaaga
     9721 gaacggacag atactgctgg catttccgcg tctttttatt gttgcccggc gtatggagta
     9781 aatatcacgt cagctggtaa tgacgatcgg gaagaatttt tgctggaatt tcggcttcat
     9841 cgttcatctg taacaggtca atttcaatag cgttgcagat ggcatccagt ggtaaatcat
     9901 tgttttcagt accgaacgga tcttccagtt cttccgccag acaatccagc gaaataaaag
     9961 tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg accagcgcga
    10021 acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg taggcaaagg
    10081 gaattggcgt ataggcaatg cgctcgcatc ctgccaggac cgctgaaata tcattaagcc
    10141 gatcgttgag gctaataaac aggatatctg aaagctgccc attgcggcgc tgaaccgcca
    10201 accactcccc cattattaac aagatacggt tagctggaga gttcgaagcc agtacaggct
    10261 gaagatcttc agtcttgaga taatgagcca gcacttccgc ctgtggctgt ttgcgtaatg
    10321 tcatgcgtaa acagtgggcg aaggcgattt gcaaccgggc aaactccctt acacttgccg
    10381 aatccggcaa tgtcgttttt acctcgcgca gtaacgaccg tgaggcaatc atcaactgcc
    10441 cccaaagttt tcgcgcttca acgtaacggg cgtacccggc attattacga aaaccaagaa
    10501 aaatggcgat ggcgacaccg agaatgctga acggtgcgag ggtgaatttg atgcccagat
    10561 gcgtgtacca gggcagcatg aaaataacag cgatagaaaa gagaaaatgg agtagtaagc
    10621 gcgatgatat cttggataat actgagccgt gccagacaaa aatacggcgc agccagtgtt
    10681 gttgtggacg aacaatcatg gttatcttca ggcgtgaaaa agtcgcccta ttaaacgtga
    10741 ttacgatcac attctcaaga cgctatttac aaattaccta caagcttaca acattaacag
    10801 gcgctgcatg tggcatctgt taatgataaa ggcacatagg gtgttgtaac taatatagtg
    10861 aaataagaaa cccggtcgaa accgggttca gaagtaacgg tgttattagc acaacggacg
    10921 tacagcttcg cgcatccctt tttcaagaat cgcatccagg tcattagcaa cctgctcgac
    10981 cagaccaggc acttgcgtca ggtcctgctc ccagtggtct ttttctgcca acacaatcgc
    11041 taccagttcc tgggtaccga taacacggtc acgatgctgg ctccagagtt gctgataacg
    11101 ttccagccag tgtgcatcgt cctgtaccgg atatgtttct ccgttacgct caccacgata
    11161 gaacgcaatc aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc cgtttgcctt
    11221 ctgccctgcc agcagctgcg gcaggatgcg ggtgcggaac ttggtcatac cgttaagcgc
    11281 gatagacagc agctgcggca ggatgcgggt gcggaacttg gtcataccgt taagcgcgat
    11341 agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg cactggcgaa
    11401 agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt agatagcttt
    11461 ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat ccagccctgc
    11521 ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca ctttgcgttc
    11581 tttgtacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt ccagacgcag
    11641 ttcagtcgct aaggatttcg gtccctgaat cacaaacagg taaaagtgtt cagcggtgtc
    11701 gagaaaacca tcgtgataac ccagctcttc ttccagttta gccacttcat cgcgcggata
    11761 accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat ccagccattg
    11821 aataaatgct tctggcagtg cccactcttg cgcatagcgc agcaccagtt cacgcaacgc
    11881 gtcgccattg tagtcgatca actcacacgg gatgatgatc caacctttat ccagcgcacc
    11941 gttgaaatgg ctaaaacgtt cgaacagcag acgggtcagt tttgccggat aacttaccgc
    12001 tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg tggtattaga
    12061 gaaaacaaag cgcatttccg ggttgtgagc cagtttcagg aattcatcgt attcactgta
    12121 gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg cttccccctt
    12181 ctcattcagg ccacggataa tggtggtata cagaccatcc tgcgtgctca gtgacggtgg
    12241 gaatgaagtt tcaatcggac gaacaacgac cacaccagaa ttcagatcgg tgtgctcatt
    12301 caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac caaactgaat
    12361 gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt tcacaatggg
    12421 ttcccttctg attagtcata caacctgttt gaattggtac gacaggttag caaactttaa
    12481 tacgccgaac ccctgttttg atcaactcct gatgattaat gagcagtttt atgagaaaag
    12541 tgtggcgcgg atcatggttt aatcgaggaa atacgccttt tcctggatca taaagtggta
    12601 gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact tcctcaccgt
    12661 aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg
    12721 cttcgaccat caatttatta aaatcatcgg tcagcgttcg gtacgatgcg cgaccactac
    12781 cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat ggttgcagtt
    12841 caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc accgacggca
    12901 ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca ccgacaatct
    12961 tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt ccatagttat
    13021 cgttaatgct tttgaaatag tcaatatcaa gcagcatgac cgtcagatgt tgtgtctgtt
    13081 tcagcgatgg acttttcagc gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa
    13141 aatcaaagtc ggctcgcagc gcaacttgct tcattagcga attgatcgct gccacgctaa
    13201 aagaaaccat aattgggcat atcgccatcg tggcaatacc gagacgtgcg gagaacattt
    13261 ccggaatgga gaacggcgaa ccgaccgaga tatcaatcac cgaatttgcc accagtacga
    13321 tttccaccgc accggtgaca aaggtcaaca gacatgttac ctgcggcgta tagcgcactg
    13381 cacaccagat taatgcaggc agcgggaacg ccagactccc cgctccgcca atgactaccg
    13441 aggcaataac cgacacaata agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg
    13501 gcagcactcc aggaatagcc aacgtcagca tacaaggcac gatcaacacg cccgttgaga
    13561 attgctcact gaaccagtcc gcaagcaaag gccagaaatc cagactatca atactgaccg
    13621 aaccaatcgc cccgacaata gcgcataata atgcggcaat cagacagtaa ttaaatagcc
    13681 gtaaggcact tactggctca tacttatttt tccccagacg cttatcccgc acgaccagta
    13741 aggcgacggt aacaataaac atcatattgg agaaattgat aaccagtgaa accagtcccc
    13801 attcggtggt tatggcatca taaaccagca tcgccacata actgatcgca tagtaatgca
    13861 gtcgattaag ccagacatag cgggcaaaca cccccgccat tacgccattc agcggccaga
    13921 aaagagaaag ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac agcgtggtta
    13981 gcgcaaaaat agcgatgcta ttacgtagca gatgaccttc ctggaacaaa cggaaagttg
    14041 aaatggggtg tacatgcatt aattatctgt ccatacaatt gcgggtggca attatctcaa
    14101 cgtaataagt aaattcacat aaaggatagc atgttcgcta acagcggcgg caaataaagg
    14161 taccctggga atgctatttt atcgttttcc gcaattctca tggctgattc atctgtacaa
    14221 ccagccagtg atagattacc gccacgatat aacgttgttg ttcttcgctt aacgaaacac
    14281 cctgcggaat gttatgccac ttagccagac aaccgcgaca gcaggtcgcg gtggcgtgct
    14341 gagcgataaa caccggatgc ccgcgcatgg gggtttgctt accgtcatta gccggtaatg
    14401 ccggagcaag acgtttagcg acaaaatccg ccgcatgttg ttcgatgact ggcgcgcctt
    14461 tctccaggca atactgacgc tctttcatgc ccagacgaaa gcgagagcga aattttgaac
    14521 gggataaacg cgcgaagagt ggatcgagag actgcattaa taaaccgaac gccctaactg
    14581 tttcgtcaat tgttcaagga cggcgatacc cgcaagcgag ttacctgcat catccagttc
    14641 cggactccag actgcgatgg ccatttcatg cggaacaatc gccacaatac cgccaccaac
    14701 gccagatttc gccggtagtc ccacccgcca ggcaaactcc cccgcgttct ggtacatacc
    14761 actggtcgcc atcagcgcgt taatttgccg cgcctgcatt ggcgtcacca caggttcatc
    14821 aatatgaata gctttcccct gattagccag aaagacaaac gtccgggcca gctctacaca
    14881 gctcattttc agagcgcagt aatgaaagta gttttgcaga acggttgtca cgtcatgatg
    14941 gaaattgcca aacgacttca tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa
    15001 ttcggaacgc gctaccaccg tatcgtatga aatatcagac acaccgctta agccgcgcac
    15061 gacttccagc atacgttgcc gtggtgcgct tagtcgccct tgcaacatat cgcagaccac
    15121 cagcgcaccg gcattaatga acggattacg cggaataccc tgttccattt ccagttgcac
    15181 taaagaattg aacggtgatc cagacggatc tttgccgacg cgttgccaga tttcctcttc
    15241 ggagtaatga cgcatggcga caacgagact cagcactttg gaaatagact gaatggaaaa
    15301 acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga tagcaatccc
    15361 caatcgggaa ccgtctactg tagccagcgc cggaatataa tccgcgactt taccctgacc
    15421 aatgagcggc cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca tggcgactgc
    15481 cactttctac tcctggaccg caggtctgaa aagacctgcg agtatatcag agctgaatat
    15541 gtcgcgtcag atccggtctt tccacaccgt ctggatatta cagaattcgt gtaagccgaa
    15601 atgggaaagc tcacgaccaa agccactctt tttcacgcca ccaaaggcca ctcgcgcgtc
    15661 gctggcacaa taaccattga taaacacccc accgcattcc agacgtgccg ccatctgtct
    15721 ggcctgtgtt tcgtcggtgg taaaaatggt cgctgaaagg ccgaactcac tatcattagc
    15781 cagttccagc gcatgttctg catctttcgc aacggtgatt gccgcaacag ggccaaacat
    15841 ttcttcccga aacgcggtca tttctggggt aacattcgcc agaaccgttg gcggatagta
    15901 gttacctgcc ccagccatct tttccccgcc cagtaacaaa cgcgcaccct gcgccagggt
    15961 tttctccacc tgatgatgca gctcatcacg taaatcaaaa cgagccattg gtccgagagc
    16021 gttctcttca tcacggggat cgcccatttt caaggctgcc gcagctgcca caaaacgttc
    16081 ggtaaatgca gaagcaattc cctcttcgat aataaagcgt tttgccgctg cacaaacctg
    16141 tccggtattc tgataacgtc cggctaccgc cgctttaacc gccagttcca gatcggcatc
    16201 gttaagcaca ataaatggat ccgaaccgcc cagttccagt acgcattttt tcagtgccgc
    16261 tccagcctgt gcgccaatag ccgctcccgc acgaacactt ccggtcaccg tgacagcagc
    16321 aatgcgcgaa tctttaatca tttgactgac accgtcgttg tcggcattca gccagccata
    16381 tacgccttgc gggattcccg catctttaaa cacctgggca atgagctgag cacagcccat
    16441 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaaccgcgcc
    16501 acgcatcacc tgccataacg gaaagttcca cggcataatc gccagaatcg tccccaacgg
    16561 tcgatactca ataactgcct gctgattttc caccagcgta ggttccgcct tcagcattgc
    16621 cggaccatgt tctgcatacc agtcacacaa attcgccgat ttcgccactt cagcgcgcgc
    16681 ctgattgatt ggtttgccca tttcacgggt gatcatttgc gccatttctt cgctacgggc
    16741 gcgcagagcc ttaccgatac cacgcagttt ttcagcacga taatctatat ttgtctcgcg
    16801 ccagtcgcga aagcctgctg ccgccagctg aagtgcgttt tcgatatcgt tagcgccagc
    16861 ccacggcagc acagaaagtt gttcacccgt ggcaggattt attgaaattg catgagttgc
    16921 cggagtaatg gtcatcgggg tatctccttt atgagtcatg gtatgaagat acgcagattt
    16981 actcttgctt taaaatgaat aatattaagc cacttattca cgaatcgaga atattatgga
    17041 tctgacacaa ctggagatgt tcaacgcggt tgccgaagct ggcagcataa cccaggcagc
    17101 agcaatagtg catcgcgtgc cgtcgaatct cactacccgt ctacgccagc tggaaacaga
    17161 actgggggta gatctgttta ttcgcgagaa tcagcgttta cgtctctctc ctgccgggca
    17221 taacttttta cgctatagcc aacaaattct cgcgttagtg gatgaagcgc ggagcgttgt
    17281 cgctggcgat gagccgcaag gtttattttc tcttggttcg ctggaaagca ccgcagcagt
    17341 gcgcattccg gccacgctgg cggaattcaa ccatcgttat cccaaaattc agttttcact
    17401 ttccaccggc ccttccggca ctatgctgga aggagtgctg gagggaaaac tgaatgcggc
    17461 atttattgat gggcccatta accatactgc cattgacggg ataccggtat accgcgagga
    17521 gctgatgatc gtcacgccac aaggtcatgc gccagtaatc cgcgccagtc aggttaacgg
    17581 cagtaacatt tatgccttcc gcgccaattg ttcgtatcgt cgccacttcg agagctggtt
    17641 tcatgctgac ggtgccgctc cgggaactat tcacgagatg gagtcgtatc acgggatgtt
    17701 ggcctgtgtg gtcgcaggag caggcattgc gcttattcca cgctctatgc tggaaagtat
    17761 gccggggcat caccaggttg aagcgtggcc gttagctgag caatggcgtt ggttaacaac
    17821 ctggctggtc tggcgtcgcg gtgcgaaaac gcgtccgctt gaggcattta ttcaactact
    17881 ggatgtgtct gactcggcaa aacagagtta ccaatgagct attttgatag ttcttgcggc
    17941 taatatgctc tatatagtga tgttccgatg acttatgact atatggggca aatatggtta
    18001 cgccagtaag catcagcaat tacatatctc ttcctgatga ttttcccgtt cgtaatattg
    18061 caccacaggt aaaagaagtt ttaaaggact ttattgatgc gctaagtaca ataatatgtg
    18121 atgaagagtg gcgcacgtct ttaaacatca actcagccac aaaaaagata tttaataacc
    18181 ttgataactt atcatatatt cagaggactt cttttcgggg taacgacacg ctatacaatg
    18241 aaaaggttca gtttaaactc acttatcccg tcaagaatgg aagacacaaa gaaaatattg
    18301 aatttcaggt agtaataaat ttaagcccta tttatttaga taattttcgc catgatggag
    18361 aaattaatat ttttgcgctc ccaacccaaa gcctgtcact atggggcgcg tatttcagac
    18421 cggcgtcgag cgtgtgctat ttctgtttct gaatgatttt attgaacaat ttccaatgtt
    18481 caaccttggc gttcccatca aaagagcgca tacaccacat attgaacccc tgcctccgga
    18541 tcgccatacc gctgcagatt acttacgcca gtttgatttg cttgtcctga attttatctc
    18601 tcgcggtaat tttgtcatac tcccccgatt atggaataac tctgaggttc acagatggtt
    18661 cgtcaataaa gatcctaact tgatcaccgc cattctcgac ataacggaca gcgaattaaa
    18721 agaggattta ttgcaaagcc tgatggattc attaggttct aacaaacatg tactacccga
    18781 agtctgcatc tgctttttat cccttttagc agaacaagaa tctcctcatt ttcaggactt
    18841 gtttttattt ttcgccaata tgttattgca ctatcaccaa tttatgaatc ccaatgaaag
    18901 tgatttgaat gacgtgttga tgccagcatc attaagtgat gataaaatta tcaaacatat
    18961 ggcgcgcagg accctcaaac tgtttgtaaa aaatgaaacg ccgccaaaag ttactcacga
    19021 agacctggtg aaaaacaggc ctcgctcccc tgtcagacca cctatacccg caaccgccaa
    19081 aacgccagac ctccctgaac gtcattaaac cgtgatgtta ccgactctct gacgcggaac
    19141 agaatcagcg tcagagaaac ggaaaacgcg atccagatca caaatgcatt gtattcacat
    19201 cattaaccgt tttaagatca tttcatcact ttttcgcaac tcacccgata atctgttatg
    19261 acaacaaaca ctgtttcccg caaagtggcg tggctacggg tcgttacgct ggcagtcgcc
    19321 gccttcatct tcaacaccac cgaatttgtc cctgttggcc tgctctctga cattgcgcaa
    19381 agttttcaca tgcaaaccgc tcaggtcggc attatgttga ccatttatgc atgggtagtg
    19441 gcgctaatgt cattgccttt tatgttaatg accagccagg ttgaacggcg caaattactg
    19501 atctgcctgt ttgtggtgtt tattgccagc cacgtactgt cgtttttatc gtggagcttt
    19561 accgttctgg tgatcagtcg cattggtgtg gcttttgcac atgcgatttt ctggtcgatt
    19621 acggcgtctc tggcgatccg tatggctccg gccgggaagc gggcacaggc cttgagttta
    19681 attgccaccg gtacagcact ggcgatggtc ttaggtttac ctctcgggcg cattgtgggc
    19741 cagtatttcg gttggcgaat gaccttcttc gcgattggta ttggggcgct tatcaccctt
    19801 ttgtgcctga ttaagttact tcccttactg cccagtgagc attccggttc attgaaaagc
    19861 ctcccgctat tgttccgccg cccggcattg atgagcattt atttgttaac tgtggtggtt
    19921 gtcaccgccc attacacggc atacagctat atcgagcctt ttgtgcaaaa cattgcggga
    19981 ttcagcgcca actttgccac ggcattactg ttattactcg gtggttcggg cattattggc
    20041 agcgtgattt tcggtaaact gggtaatcag tatgcgtctg cgttggtgag tacggcgatt
    20101 gcgctgttgc tggtgtgcct ggcattgctg ttacctgcgg cgaacagtga aatacacctc
    20161 ggggtgctga gtattttctg ggggatcgcg atgatgatca tcgggcttgg tatgcaggtt
    20221 aaagtgctgg cgctggcacc agatgctacc gacgtcgcga tggcgctatt ctccggcata
    20281 tttaatattg gaatcggggc gggtgcgttg gtaggtaatc aggtgagttt gcactggtca
    20341 atgtcgatga ttggttatgt gggcgcggtg cctgcttttg ccgcgttaat ttggtcaatc
    20401 attatatttc gccgctggcc agtgacactc gaagaacaga cgcaatagtt gaaaggccca
    20461 ttcgggcctt ttttaatggt acgttttaat gatttccagg atgccgttaa taataaactg
    20521 cacacccata cataccagca ggaatcccat cagacgggag atcgcttcaa tgccaccctt
    20581 gcccaccagc cgcataattg cgccggagct gcgtaggctt ccccacaaaa taaccgccac
    20641 caggaaaaag atcagcggcg gcgcaaccat cagtacccaa tcagcgaagg ttgaactctg
    20701 acgcactgtg gacgccgagc taataatcat cgctatggtt cccggaccag cggtacttgg
    20761 cattgctagc ggcacaaagg caatattggc actgggttca tcttccagct cttccgactt
    20821 gcttttcgcc tccggtgaat caatcgcttt ctgttgcgga aagagcatcc gaaaaccgat
    20881 aaacgcgacg attaagccgc ctgcaattcg cagaccggga atcgaaatgc caaatgtatc
    20941 catcaccagt tgcccggcgt aatacgccac catcatgatg gcaaatacgt acaccgaggc
    21001 catcaacgac tgacgattac gttcggcact gttcatgttg cctgccaggc caagaaataa
    21061 cgcgacagtt gttaatgggt tagctaacgg cagtaacacc accagcccca ggccaattgc
    21121 tttaaacaaa tctaacattg gtggttgtta tcctgtgtat ctgggttatc agcgaaaagt
    21181 ataaggggta aacaaggata aagtgtcact ctttagctaa ccttgcatcg cattgaccaa
    21241 aacttgaacc gatttagcaa aacgtggcat cggtcaattc attcatttga cttatacttg
    21301 cctaggcaat attatcccct gcaactaatt acttgccagg gcaactaatg tgaaaagtac
    21361 cagcgatctg ttcaatgaaa ttattccatt gggtcgctta atccatatgg ttaatcagaa
    21421 gaaagatcgt ctgcttaacg agtatctgtc tccgctggat attaccgcgg cacagtttaa
    21481 ggtgctctgc tctatccgct gcgcggcgtg tattactccg gttgaactga aaaaagtgtt
    21541 gtcggtcgac ctgggagcac tcacccgtat gctggatcgc ctggtctgta aaggctgggt
    21601 tgaaaggttg ccgaacccga atgataagcg cggcgtactg gtaaaactta ccaccagcgg
    21661 cgcggcaata tgcgaacaat gccatcaatt agttggccag gacctgcatc aagaattaac
    21721 aaaaaaactg acggcggacg aagtggcaac acttgagcat ttgcttaaga aagtcctgcc
    21781 gtaaacaaaa aagaggtatg acgatgtcca gacgcaatac tgacgctatt accattcata
    21841 gcattttgga ctggatcgag gacaacctgg aatcgccact gtcactggag aaagtgtcag
    21901 agcgttcagg ttactctaaa tggcacctgc aacggatgtt taaaaaagaa accggtcatt
    21961 cattaggcca atacatccgc agccgtaaga tgacggaaat cgcgcaaaag ctgaaggaaa
    22021 gtaacgagcc gatactctat ctggcagaac gatatggctt cgagtcgcaa caaactctga
    22081 cccgaacctt caaaaattac tttgatgttc cgccgcataa ataccggatg accaatatgc
    22141 agggcgaatc gcgcttttta catccattaa atcattacaa cagctagttg ataacgtgac
    22201 aacgtcactg aggcaatcat gaaaccactt tcatccgcaa tagcagctgc gcttattctc
    22261 ttttccgcgc agggcgttgc ggaacaaacc acgcagccag ttgttacttc ttgtgccaat
    22321 gtcgtggttg ttcccccatc gcaggaacaa ccaccgtttg atttaaatca catgggtact
    22381 ggcagtgata agtcggatgc gctcggcgtg ccctattata atcaacacgc tatgtagtta
    22441 ttgtcaggct ctgatcacag agcctgacat ctccgtaata tcttcctcaa gtctcaaata
    22501 ttttcgccat tgttggcgat gagatcccga taccagtaaa acgacgcttt acgtatccgt
    22561 tccagcgttc ccttcccttc gttgtcttta tcgacataaa tcattccgta gcgctttttc
    22621 atttcccctg ttccggcaga aaccaggtca atgcagcccc acggggtgta gccaattaag
    22681 tcaacaccat cttcaactac ggcttttttc atttcccgaa tatgggaagc aaagtaatca
    22741 atgcgatagt gatcgttcac cgtgccgtca gcttgtctct ggtcaaccgc accaaatcca
    22801 ttttcgacaa taaacagcgg caactggaaa tgatcccaga accagttgag tgaataacgt
    22861 agccctgccg gatcaatttg ccatccccat tcggatgtat cgatataagg gttgctgacc
    22921 aaatctcgtg gttcaacata atccagttgc gggttatcgg gcgaaaattg tgtcgtaaaa
    22981 gacatgtagt agctaaagcc gataaagtcg acacaaccac tggcaagaat cgcgttatct
    23041 tctggtgtga tatcgaggtt gaatcctttc ctggcaaagt aattcagcat atgttgcgga
    23101 taatatccac gagtatgaac atcagtaaac cagtaacgac gatgcattgc tttcgtggcc
    23161 atcatcatat cgttgggtgc acacgtcaga ggatagatgg gacacatggc gatcatacag
    23221 ccaatgttaa attcaggatt gatcgatttt ccagtctgta ccgccagggc actggcaact
    23281 aactcgtaat gtaccgcctg gtacattatt tgttcgcgct cattgatatc ttcctctggc
    23341 gaatacaaga taccggaatt agtaaatgga cacaggcttt cgctgaaatt cacctgatta
    23401 ttgatttcgt taaacgtcat ccagtacttt actttttctt tatagcgcgt gaagaccgtt
    23461 gatgcgaagc ggataaaaaa gtcgatcagt ttacggtttc gccagccacc atattttgtc
    23521 accagatgat aaggcatctc aaaatgtgaa agcgtcacca caggttccat tccctgctta
    23581 aggcattcat cgaacagatc atcataaaat tgtaaaccct cttcattcgg ctcctgttcg
    23641 tcaccttgcg gaaagattcg tgtccaggca atggaagttc gaaagcattt gaatcccatc
    23701 tcggcaaata actgaatatc tgttttatag cgatgataaa aatcaattgc ttcataattg
    23761 ggataattaa gcccgtcgat aacgccttct gtcacttcac gcggcacccc gtgagcgcca
    23821 gcagtcatca catcagcgat gctgatgcct tttcctcctt cattccagcc accttccaac
    23881 tgatgcgcgg ctaccgcgcc gccccataaa aaaccttttt taaatcctga catacacaac
    23941 tccttaacta aaatagatta ccaccagact tccgcctgga tgccggccat aaactcgtct
    24001 ttgctgttct cattaaactt aaaattcgat aattcgttat ccagaatatt gatatacgtt
    24061 ccatagaagc gaatttctgg tcgtgaacct aaaatgcttt cacccacttt caatgcatgc
    24121 cagagtgtcg ttttataagc cgattcattt agtgttacac cctgctgagt tttgttctgt
    24181 tgcttaaacc agcctaattc aagccccgtc tgattccatg tattccagat ccaggccggt
    24241 cttattacag tgcgaatact gtcaaaatca ctatgagcac cactttcata actataaaca
    24301 tcttcgccgt gagaatagac aagcgcgtta gccataataa tattgtcagt aagatacatc
    24361 tcgccctgag agattaaacg ccaggcgatc ccattggtat gatcaccata gtaatagcga
    24421 ccatgcgcca tcgtattact ggcatctgag aaactggcaa aactgctggc ataggaatta
    24481 ttggcaacct gtaacgtaaa ttcattaaac gtatcgcgtt gcaggttttg ccgtaaaaca
    24541 gaagtcagca tccagctatc tttcatttca aaataactgt tgtcattttc gttatcttgt
    24601 tgttgatccg ttttattagg tgcggaatat ttagccatta atgataatgt cgcatcatcc
    24661 cataacggga tattgcgata acgaacatcc acagaattgg tattcatctg gctggtacgc
    24721 gaaaaatcac gagagtaaac atcgacatca tcacggctta aggacatatc aaacagccct
    24781 acaccaagtg cccagttttc aatccccaca cccgcggcga catccgtggt taaggttttc
    24841 cagtccagca tctggatctc atattgcggg agtttatgtt tgccgaccca gaagtctgcc
    24901 tctggtgcga agggtaaaaa acctcgcgtt gtcaggtaaa tatcgctgaa ctgcatgata
    24961 ttttcattgg cactgtcacc aaaccaggca tcgttatact gctcacccac gttgccatca
    25021 taggtaacga ccgcatttgc cgttttaccg tcctgattat aaacacgctg atttaaggtc
    25081 aggtcaaacc aaccgctcat ctcattacca aaacgtccca gagaacccgc tgcataagtt
    25141 tgtggtgagc cgtgattacc agtaccccag cctgagcgga agtacccctg atagctgaac
    25201 cca
//
LOCUS       DAZFWP020000069        24938 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFWP020000069 DAZFWP020000000
VERSION     DAZFWP020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.069"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1030
                     /locus_tag="ACHK0Z_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             <1..1030
                     /locus_tag="ACHK0Z_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4502581.1"
                     /translation="TLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKK
                     TGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAF
                     LLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFV
                     VSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQ
                     FKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIA
                     ERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGG
                     NKVIIHHI"
     gene            1211..2701
                     /gene="dgcJ"
                     /locus_tag="ACHK0Z_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             1211..2701
                     /gene="dgcJ"
                     /locus_tag="ACHK0Z_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU4502582.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEKRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2744..3247
                     /gene="yeaK"
                     /locus_tag="ACHK0Z_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             2744..3247
                     /gene="yeaK"
                     /locus_tag="ACHK0Z_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU4502583.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3248..3352)
                     /gene="yoaI"
                     /locus_tag="ACHK0Z_RS12920"
                     /old_locus_tag="pgaptmp_002584"
     CDS             complement(3248..3352)
                     /gene="yoaI"
                     /locus_tag="ACHK0Z_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU4502584.1"
                     /translation="MNDQMFVETLIISSSFFAIAVVLVLSVLLIERTG"
     gene            3522..3968
                     /gene="yeaL"
                     /locus_tag="ACHK0Z_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             3522..3968
                     /gene="yeaL"
                     /locus_tag="ACHK0Z_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU4502585.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3925..4746)
                     /gene="nimR"
                     /locus_tag="ACHK0Z_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             complement(3925..4746)
                     /gene="nimR"
                     /locus_tag="ACHK0Z_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU4502586.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVIMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWACFFAMSERNIARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4843..6024
                     /gene="nimT"
                     /locus_tag="ACHK0Z_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     CDS             4843..6024
                     /gene="nimT"
                     /locus_tag="ACHK0Z_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU4502587.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6079..6426
                     /gene="yeaO"
                     /locus_tag="ACHK0Z_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     CDS             6079..6426
                     /gene="yeaO"
                     /locus_tag="ACHK0Z_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU4502588.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6448..6702)
                     /gene="yoaF"
                     /locus_tag="ACHK0Z_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     CDS             complement(6448..6702)
                     /gene="yoaF"
                     /locus_tag="ACHK0Z_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU4502589.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRYHPAGEK"
     gene            6885..7910
                     /gene="dgcP"
                     /locus_tag="ACHK0Z_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     CDS             6885..7910
                     /gene="dgcP"
                     /locus_tag="ACHK0Z_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU4502590.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLDDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7943..8041
                     /gene="yoaK"
                     /locus_tag="ACHK0Z_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     CDS             7943..8041
                     /gene="yoaK"
                     /locus_tag="ACHK0Z_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU4502591.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8044..8118
                     /gene="yoaJ"
                     /locus_tag="ACHK0Z_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     CDS             8044..8118
                     /gene="yoaJ"
                     /locus_tag="ACHK0Z_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU4502592.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8177..8425)
                     /gene="yeaQ"
                     /locus_tag="ACHK0Z_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     CDS             complement(8177..8425)
                     /gene="yeaQ"
                     /locus_tag="ACHK0Z_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4502593.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8653..9861)
                     /locus_tag="ACHK0Z_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             complement(8653..9861)
                     /locus_tag="ACHK0Z_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4502594.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            9869..10300
                     /gene="tnpA"
                     /locus_tag="ACHK0Z_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     CDS             9869..10300
                     /gene="tnpA"
                     /locus_tag="ACHK0Z_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HGU4502595.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(10316..10504)
                     /locus_tag="ACHK0Z_RS12980"
                     /old_locus_tag="pgaptmp_002596"
     CDS             complement(10316..10504)
                     /locus_tag="ACHK0Z_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU4502596.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10508..10867)
                     /gene="yeaR"
                     /locus_tag="ACHK0Z_RS12985"
                     /old_locus_tag="pgaptmp_002597"
     CDS             complement(10508..10867)
                     /gene="yeaR"
                     /locus_tag="ACHK0Z_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU4502597.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11038..11676)
                     /gene="leuE"
                     /locus_tag="ACHK0Z_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     CDS             complement(11038..11676)
                     /gene="leuE"
                     /locus_tag="ACHK0Z_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU4502598.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIIRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLTKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(11803..12726)
                     /gene="dmlR"
                     /locus_tag="ACHK0Z_RS12995"
                     /old_locus_tag="pgaptmp_002599"
     CDS             complement(11803..12726)
                     /gene="dmlR"
                     /locus_tag="ACHK0Z_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU4502599.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYQNFSLEHA"
     gene            12829..13914
                     /gene="dmlA"
                     /locus_tag="ACHK0Z_RS13000"
                     /old_locus_tag="pgaptmp_002600"
     CDS             12829..13914
                     /gene="dmlA"
                     /locus_tag="ACHK0Z_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU4502600.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            14165..15775
                     /gene="yeaV"
                     /locus_tag="ACHK0Z_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     CDS             14165..15775
                     /gene="yeaV"
                     /locus_tag="ACHK0Z_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU4502601.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFLTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            15807..16931
                     /gene="yeaW"
                     /locus_tag="ACHK0Z_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             15807..16931
                     /gene="yeaW"
                     /locus_tag="ACHK0Z_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU4502602.1"
                     /translation="MSNLSPDFVLPENYCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            16987..17952
                     /gene="yeaX"
                     /locus_tag="ACHK0Z_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             16987..17952
                     /gene="yeaX"
                     /locus_tag="ACHK0Z_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU4502603.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18006..19121)
                     /gene="rnd"
                     /locus_tag="ACHK0Z_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             complement(18006..19121)
                     /gene="rnd"
                     /locus_tag="ACHK0Z_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU4502604.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(19203..20888)
                     /gene="fadD"
                     /locus_tag="ACHK0Z_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             complement(19203..20888)
                     /gene="fadD"
                     /locus_tag="ACHK0Z_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU4502605.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(21093..21674)
                     /gene="yeaY"
                     /locus_tag="ACHK0Z_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             complement(21093..21674)
                     /gene="yeaY"
                     /locus_tag="ACHK0Z_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU4502606.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(21714..22409)
                     /gene="tsaB"
                     /locus_tag="ACHK0Z_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             complement(21714..22409)
                     /gene="tsaB"
                     /locus_tag="ACHK0Z_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU4502607.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(22467..24377)
                     /gene="yoaA"
                     /locus_tag="ACHK0Z_RS13040"
                     /old_locus_tag="pgaptmp_002608"
     CDS             complement(22467..24377)
                     /gene="yoaA"
                     /locus_tag="ACHK0Z_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU4502608.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLHSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIAIQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            24509..24853
                     /gene="yoaB"
                     /locus_tag="ACHK0Z_RS13045"
                     /old_locus_tag="pgaptmp_002609"
     CDS             24509..24853
                     /gene="yoaB"
                     /locus_tag="ACHK0Z_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4502609.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         6073 a         6374 c         6061 g         6430 t
ORIGIN      
        1 tacactgatc cagacaaagt cgaatgatgt ttctattttc tatattttcc gccaactcag
       61 ttttatttgt ttaacctcgc tggcgctctt ttgttatggt aaagacaaca tccttgacaa
      121 caataagaaa aaaactggca ttctgttgct ggcgctgatt ccttttttag tttttcccct
      181 tctggcacac aatctgagca gttataacgc tgactattct ttgtatgtcg tcgattactg
      241 tccggacaac catactgcga cctggggaat caactataca aaaatattgg tttgtctgtg
      301 ggcattttta ctgttcttta ttatcatgcg tacacgatta gccagcgaac tatggccgtt
      361 aatagcatta ttatgtctgg catcgctatg ctgcaactta cttctactga ctctggatga
      421 gtataattac accatctggt atatcagtcg cgggattgaa gtttccagta aactgtttgt
      481 tgtgtctttt ctgatttata acatttttca ggaactgcaa ctctccagca aactggcagt
      541 tcatgatgtg ctgaccaata tttataatcg gcgctacttt ttcaacagcg tagagtcatt
      601 attgtcgcga cctgttgtta aggacttttg tgtcatgctg gttgatatta atcagttcaa
      661 acgcatcaat gcccaatggg gacatcgtgt gggtgataaa gtgctggttt caattgtcga
      721 tattatccag caaagcatcc gccccgatga tattttagcg cgactggagg gtgaggtgtt
      781 tggcttgcta tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg aacgtatgcg
      841 taaaaatgtc gaactcctga ccggctttag taacagatat gatgttcctg aacaaatgac
      901 catcagtatt ggcacggttt tttcaacggg tgacacgcgt aatatctcgc ttgtcatgac
      961 ggaagcagat aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag tgattattca
     1021 tcatatttaa gtgcaaaaat attcagagcc atgctttttg cgtggctttt gcatacaatt
     1081 tattactatt acccctaaat ttcccctccc cagcggcgca gagatgagta taattagcgc
     1141 ccctgtgcca ggccgcaatc gaactttatc tggttttctc gtttcactaa ccgaaggagt
     1201 gccatttatc atgaaattgc accatagaat gctccggcat tttatcgccg caagtgtcat
     1261 tgtgctgaca tcttccttcc ttatttttga acttgtcgcc agcgacagag caatgagcgc
     1321 ctatctgcgc tatattgtgc agaaagcaga ctcctccttt ctttatgata agtatcagaa
     1381 tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct gagcagtcgg aagtgtcgcc
     1441 agaaaagcgg cgcgccatct gcgaggcttt tgagtctgcc aataataccc atggcttaaa
     1501 cctgactgcc cataaatacc cgggcttacg cggcacacta caaaccgcat ccactgactg
     1561 cgacacaatt gtggaagctg cagcgctatt acccgctttt gatcaggcag tggaaggcaa
     1621 ccgccaccag gatgattacg gttcaggtct tgggatggcc gaagaaaaat ttcactatta
     1681 tctcgatctc aatgaccgct atgtctattt ttatgagccg gttaatgttg aatactttgc
     1741 gatgaataac tggtccttcc tgcagtcagg aagtattggc atcgatcgca aagatattga
     1801 aaaggtattt accgggcgta ccgtattgtc gagcatttac caggatcagc gtactaaaca
     1861 gaacgtgatg agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct
     1921 gctggatatt aacaaaaaca atctgcggaa tatcttttat actcatgacc gccctctcct
     1981 ctggcgtttt ctcaatgtca cgctaaccga taccgattcg gggcgcgaca ttatcatcaa
     2041 ccagagcgaa gataatctgt tccagtatgt cagttacgtc catgacttac cgggcggcat
     2101 tcgtgtctcg ttatccattg atattcttta ctttatcacg tcttcgtgga aaagcgttct
     2161 gttctggatt ttgacggcgt taattttgct gaatatggtc cggatgcact tccgtttata
     2221 ccaaaatgtg tcgcgagaaa atattagtga tgcaatgact ggactgtata atcgcaaaat
     2281 tttaacccct gaactggaac agcggttgca gaaactggtg caatccggtt cttcggtgat
     2341 gtttattgct attgacatgg acaagttaaa gcaaataaat gacaccctcg gtcatcagga
     2401 gggggattta gcgattacgt tattagctca ggcgattaaa cagtcgattc gtaaaagtga
     2461 ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt cgacgccgca
     2521 aattgcagca caactgcctg aacgtatcga aaaacgtctg caacatatcg cgccgcagaa
     2581 agagatcggc ttctcttccg gtatttatgc gatgaaagaa aacgatacgt tacatgatgc
     2641 gtataaagct tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg
     2701 ataaccttct gtggttgttt gcttgtaatc tcaggagcat gaaatgactg aaatggctaa
     2761 aggaagcgtg acgcatcaac ggttaatcgc tttattatca caagaaggtg ctaacttccg
     2821 cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggcaccgc
     2881 tttaggccag ggagctaagg cactggtatg taaggtcaaa ggcaatggcg taaatcaaca
     2941 tgttctggcg atcctcgctg ccgatcaaca ggccgatctg agccaactcg ccagccatat
     3001 aggcggatta cgcgcttcgt tggccagccc ggcggaagtc gatgaactca caggctgtgt
     3061 cttcggcgcg atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccctct
     3121 actcttcgaa cgatttgatg aaatcgcctt caatgcaggc atgctggata aatccgttat
     3181 tttgaaaacc gcggattatt tgcgcattgc gcaaccagaa ctggtcaatt tccgccgcac
     3241 tgcgtaacta gcctgtccgt tcaataagca gaacggacaa aaccagcaca accgcaatgg
     3301 caaaaaacga tgacgagata atcagtgtct cgacgaacat ttgatcgttc atagtatgcc
     3361 cccgggagat agctgttttc acgttattgt tagcgtgcac caatggcagt ttgatgacag
     3421 ttcgcctgat tttttatgca aaaaacgtaa agattttcta cttccttcct gcagcaagcg
     3481 taaagtaagc aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc
     3541 tgatcctgct cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt
     3601 caattctggt gttgatcatt gtccgcgtca caccgttaag cacctttttc ccgtggattg
     3661 aaaaacaggg gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatcg
     3721 ccagcgggac gctaccgccc tcaaccctga ttcactcgtt tctgaactgg aaatcgctgg
     3781 tggcaattgc agtaggcgtt attgtctcct ggctgggtgg gcgcggcgtg acgttaatgg
     3841 gcagccagcc gcaactggtt gctgggctgc tggtgggcac tgttttgggc gtggcgctgt
     3901 ttcgcggcgt accggttggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga
     3961 aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat
     4021 agtaataaag gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc
     4081 gcctttcact aatccctgaa gcgccataat cagctgtaac tgctgtcgcc actggcgaaa
     4141 actcaacccc gtctctttta ctatcagtcg ggcaatattg cgttcactca tcgcaaaaaa
     4201 gcaagcccat tgccccaatg acccccactc gacgggctct ttcgccatca tctccaccat
     4261 cgcgcggatt ttaggatgag aagaaacggg taggtgcagc tgttgttgtg gctgctgagg
     4321 gagttcatca aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt
     4381 cgtcctgttt gccagagtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca
     4441 acaggttgtc ggcattatca ctgcgctggg ttcgataaac agaaaacaga gttcagcgtt
     4501 agctgtcact tgattgctat gttccacccc tcccggtatc caaaccgcat attgcggcgg
     4561 caccatccac aaagcatttt ccaccgtaca ggtaattgca ccatgtagcg ccagtatcag
     4621 ctgtcctttg cggtgctgat gtacaggact ggttaactca tccgttccgg cgtgaataca
     4681 aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag ccattgagat tcaacctgtg
     4741 catcattttg tccgaattta gcgataattt gtcattttag cttgattcaa cataacaata
     4801 aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc atatgacctg ttcaacttca
     4861 ttaagcggca aaaacaggat tgtccttatc gctggcattc tgatgattgc cacaacatta
     4921 cgcgtcacct ttaccggcgc agcgccgtta ctggatacga ttcgttccgc ttactcgctg
     4981 acgacagcgc aaaccggctt attaaccacc ctgccattat tggcctttgc gctaatctca
     5041 cctttggctg ccccggtggc gcgacgtttt ggtatggaac gtagcctgtt tgccgcgtta
     5101 cttttgatct gtgcaggtat cgcaattcgt tctctccctt cgccttactt attatttggc
     5161 ggtacagcgg tcattggcgg tgggattgca ttaggcaatg tcttactgcc aggattaatt
     5221 aaacgcgatt tccctcattc cgtcgccaga cttaccggcg catattccct gacaatggga
     5281 gctgcagcgg cactgggatc ggctatggtc gtgccgctgg ctttgaacgg ttttggctgg
     5341 caaggcgcgt tgttcatgct gatgtgtttt cctctgctgg ctcttttttt atggctgcca
     5401 cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc gcgccttaca tactcggggt
     5461 atctggcgtt cgccgcttgc ctggcaggtc acattgtttc ttgggatcaa ctcactggtc
     5521 tattacgtga ttattggctg gcttccggcg atcctcatca gtcgcggcta tagcgaagca
     5581 caggcgggtt cactgcatgg tttgctgcaa ctagccacag cagctcccgg tttgctgatc
     5641 cctcttttct tacatcatgt gaaagatcag cgtggtattg cagcgttcgt tgccttgatg
     5701 tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc acgcgatcac ctggactctg
     5761 cttttcggtt ttggttccgg cgcaacaatg atactggggt tgacgttcat tggtcttcgg
     5821 gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg
     5881 ctggcagcct gtgggccgcc gctgatgggt aaaatacacg atgctaacgg tgactggtcc
     5941 gtaccactga tgggtgttgc catactttca ctactgatgg cgattttcgg actttgcgcc
     6001 gggagagaca aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcatgcgtg
     6061 aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag
     6121 agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat
     6181 ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt
     6241 cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat atcttgcaga actggcgcaa
     6301 cacgagcaag aaggaaagcg gctggcggac atcgccaaaa aacagcccct gaccctgctc
     6361 tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc
     6421 ttgtgatttt agtacagcat ccggcggtta tttttcacca gccggatggt accgtcgcca
     6481 caatgcccat tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg
     6541 cgctgtctgc actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt
     6601 tgccatacca atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc
     6661 caggaccagc aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt
     6721 tatccagact tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc
     6781 actcaaatca tattacgcct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa
     6841 gattaaataa tcagcattta cccgccgtat cctggagttg tttcgtgtca gatcagatta
     6901 tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc
     6961 ttctggagat gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg
     7021 tcgaagcgcg cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg
     7081 agaattttac cgtctcgtgg gattactcgt tatgcaaacg cgccattgat gaaaattgct
     7141 ttttcagcga tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggca
     7201 tcaccacatt tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt
     7261 gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat
     7321 tcgctggact gattgcacaa tatattcaaa aagaggcgct ggttgaacag ctgcgcgaag
     7381 ccaatgctgc gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc
     7441 gggcgatttt tgaaaatctg acgacgctgt tttctctcgc ccggcatctt aaccataaga
     7501 taatgatcgc gtttatcgat ctggataact tcaaattaat caatgaccgt tttggtcata
     7561 atagtggcga tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg
     7621 gcgaagttat tggtcgtctc ggcggtgatg agtttttggt tgtttcacta aacaacgaga
     7681 atgcggatat ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact
     7741 tagatgatgt tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg
     7801 aaacaaccga tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa
     7861 aacacaaaca gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca
     7921 catccttttg attggtgata acatgcgaat cggtattatt tttccggttg taatctttat
     7981 tacagcggtc gtatttttag catggttttt tattggcggc tatgctgccc cgggagcata
     8041 aagatgaaaa aaacaacgat tattatgatg ggtgtggcga ttattgtcgt tctcggcact
     8101 gagctgggat ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct
     8161 ttgtgcaatt taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatcgcac
     8221 caataacggc aaccacgaag ctgccaaaat tgaagccatc gactttacca aagccaaaca
     8281 gtgtgctgat ccatccgccg actacggcac cgactatccc cagcagaata gtcataaaga
     8341 atccaccacc atctttacct ggcatgatcc acttcgccag aataccggca ataagcccaa
     8401 aaataatcca tgacagaatg cccattgttt cctcacttat ctgttttgca ttagcgggtt
     8461 agtcgctgat aaaaagcata gcacaacatc gggagggcaa gatttgtgac gagcatcacg
     8521 gaggattttt ttgcggatgg cacagaaatt gcgccatcaa cgatcagtga taattaccaa
     8581 ccacaaacat catgttcgtt ttccgtgtct tgacatcctc cacgccctga atgacgagga
     8641 tccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
     8701 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
     8761 agcgccgttt acatcggcgt tcgctgtata tccacatacc tggcatctga attgactttg
     8821 tgacaggcga ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
     8881 tggcggaaca gcaagcacct gaccgccacg ccagagctgc ttgtactcaa gctggcggcg
     8941 catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt
     9001 gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc
     9061 ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc
     9121 cctgcggata tttgcgatac gggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
     9181 gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca gcgtcttctg
     9241 gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg tggcgagttt
     9301 agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag ttgatacttc
     9361 actttctgtc tgaatactga tgtaccactt accgcaggac tggctgacag tgacattttt
     9421 cacaacaccc gtgacctgac ggctattccg gtagcgcatc cagcccagtt tcggcagaaa
     9481 aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaatg catcattctg
     9541 tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt tgtaagcccg
     9601 ctcaaggtct ttcagtgact gttgcaatgg ctgtgaggga gaatctttaa gccattgcgt
     9661 ttcagtggca tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
     9721 attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
     9781 tgcgcctgcg aagcgcctca tttcacactc ctgttgatca cctggtctta actggaattt
     9841 aaatgcctgt agtcgtttca caggccaaat gatactttgg tctgtgaaaa aagaaaccga
     9901 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccag
     9961 atacagacgt cagatttttg accatgacgc gacagaaaaa ctacgcgctt acttttcaaa
    10021 tgtatgtgct gattttgaag ctgaacttgt tgaaatggat ggcgaaccag atcacgtcca
    10081 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
    10141 cgtatcgggt aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
    10201 aggcgttttg tggagtcctg gctattttgc cagtagctgc ggaggtgcgc caatatccgt
    10261 catccgccaa tacattgaac aacagcaaac acctggttag gtggaaaacc gcgccttata
    10321 tccccgccct gaaggacggg gttttacggc gcaccggata agaacgcacg gtattcacca
    10381 gatcttttat cacttcagcc gccacttctg gcaccagcaa agtcatcggc gtctctgttt
    10441 cataatcgac agaaacgcca ttgctgttat tggtgacggt cacggtatac gttgctttgc
    10501 ccatgattca tttcccgtta tgaatgactt tccgttgttg cgcaccttcc atcaggactt
    10561 caggagccac gaagaagtca atgttgaaat aagtatcgtc agtcatggct tcaatgttgt
    10621 gccacttttc tggagggaac accgcaaact gccccgcttc gataaggatc acctgatcag
    10681 gctctgcact gtgttcatca gcgtagccga gatatttgac cgccccatgc ataacggaaa
    10741 ggcgtgggta aacccccggg cgcgttcctt tatcaagatg acgttcgaat attccggcag
    10801 gcgcagtttg tttattccag aaaggcgttg agcgcgtatg aatataattc tgtgggattt
    10861 gcagcatctt tttccctcct tcgttgaatg cgctgaaaac ggtattccag ccgtttcaga
    10921 gtacgcctga taatttgcat tttaaatacc atttattggt tattttttag catcatatca
    10981 gagaagaatc agggaggatt acaagtggga agcccgcgac aacgcgggct gaatgtatca
    11041 ggattgcagc gtcgccagtc gggcagcgaa acccacgaac atcaaaccaa tcagtgagtt
    11101 gcccacttta gtcagtttct ttttggtacg tatgtactgc gtgacaaaag caccagaaat
    11161 aatcaggaag ctcaaatagc agaaactcac cagttccagc gtcgtcgcca gaataaagaa
    11221 tgaaattccc gtatgtgggg cattaacatc gataaactgt acgaaaaacg acacatagaa
    11281 caaaatggct ttcggattag tcaggctcaa aattaacgcg cgtttaaaaa tagcaccgta
    11341 ttggggctca tcggatttgg cctcgttatt tttacccttc agggtcgcgt agagaatttt
    11401 actccccaga tagagcaaat aaaacgcacc aagataacgt ataatgttga ataatatcgg
    11461 ggtggtctta attaatgtcg ccactccagc ccatgccaga aacatcaata ccgcatcgcc
    11521 aataaataca ccgcaggccg caagataacc gcctttcata ccgctactga cgctattttt
    11581 gagtacaaac agggtatttg gccctggcac caacacaata aaaatggccc caaccagata
    11641 ggtccagtaa ttcagaaccc cgtattcagc gaacacatta acctctttaa ttatctttcg
    11701 atcatgcgcg attaaaggtg aatatgctaa ccaatctgta gcggcttaga aaggagaaaa
    11761 tcaggtttta acccgatatc aacccgataa ttgaataatt aatcaggcat gctccagtga
    11821 aaaattctgg tagtgctcgg caaaatactg gcgtaaaaat tctaccgtta tccgcacttt
    11881 cgctgacgtc gccagccttg aaacataaac ggcccagacg ttcgctggct ggtaatattc
    11941 cggtagcact tgcactaaat gaccactggc aatgttttcg ctaacatccc accaggaacg
    12001 cagagcaatc ccctgcccat cgagacacca ctgatggaca atttctccat gattagacga
    12061 cagtgggccg gtgactttaa tcgcatggtg gccttcttta ttgcgtaatt gccagacacc
    12121 aaaaggatga tcgcgctctt taatgaccaa gcacggcaat gcagaaagat cggttaaatg
    12181 tttcggcgcg ccatgttgcg caataaattc cggcgacgcg cagagaatgc ggtaattggt
    12241 cgccagtttg cgggcgatta aattaggggc gatgtcatcg ccaatgcgaa tatcgagatc
    12301 gacaccttca ttgaccaaat cgaccagtcg gtcttccaca tcaaaacgta attccagttg
    12361 cggatacgcc ttcgccagcg ctgataacgc cggagccacc acctgtcgtc caaaaccaaa
    12421 gctgctgata atacgcagca tcccctgggg cacctgacgc acgtcagaaa gttcgtccat
    12481 catctgaccg acatcctgca aaatccgctg cgcccattca taaatccgct ctccttcttc
    12541 ggtaatggtg acgcggcggg tggtgcggtg taacaacacc acgtttagcg tttgctccag
    12601 caaggcgatg cgcttgctga cgaacgccgg tgaaacgccc agttcttccg ccacggcggc
    12661 aaaaccagcc cgacgagcca ccagcataaa gacgcgcaaa tcattcagca gcggtaaatt
    12721 attcatgatt cgtgttttat gtttcaccag ttacggggat taattccttt tcagtcaatt
    12781 ataggatggt gatgttgtca attttgatgg tcaggaagtg agaacccaat gatgaaaacg
    12841 atgcgtattg ctgcgatccc gggagacggg attggcaaag aagtccttcc tgaagggatt
    12901 cgcgtgttac aggctgccgc tgagcgctgg ggcttcgact tgagttttga gcaaatggag
    12961 tgggcgagct gcgagtatta cagccatcac ggtaaaatga tgccggacga ctggcatgag
    13021 caacttagcc gtttcgacgc catctatttt ggtgccgtcg gctggccgga taccgttccg
    13081 gaccatattt cgttgtgggg ttcgctgctg aaatttcgtc gtgaattcga ccagtacgtc
    13141 aacctgcgcc cggttcgtct ctttcctggc gttccctgcc cgctggcggg aaaacagcct
    13201 ggcgacatcg atttttacgt ggtcagggaa aacaccgaag gcgaatattc ctcgctcggc
    13261 ggtagagtga atgaaggtac agagcatgaa gtcgtcattc aggaatcggt atttacgcgt
    13321 cgtggtgtcg atcgcatttt gcgttatgcc ttcgaacttg cgcaaagccg cccacgtaag
    13381 acgctaactt ctgccactaa atcaaacggt ttagccatca gcatgccgta ctgggatgag
    13441 cgagtggaag caatggccga gaattacccg gagatccgct gggacaagca gcatattgat
    13501 attctctgcg cgcgttttgt gatgcagccg gaacgcttcg atgtggtggt ggcgtccaat
    13561 ttatttggcg atatcctttc cgatcttggc ccggcctgca ccggcaccat tggcattgcc
    13621 ccatccgcca acctgaatcc ggaacgcact ttcccgtcgc tcttcgagcc agtccacggt
    13681 tccgcgccgg atatctacgg gaaaaacatt gctaacccta tcgccacgat ttgggccggt
    13741 gcaatgatgc tcgattttct cggcaatggc gatgagcgtt tccagcaagc gcataacggt
    13801 attctggcag caattgaaga agtgattgct cacgggccga aaacacctga tatgaaaggc
    13861 aatgccacca cgccacaggt tgccgacgcg atttgcaaaa ttattttgcg ttaaggtcaa
    13921 accagtttat ttgaaccgcg tcactgacgc ggttttttta ttcgttcttt gcagtaaata
    13981 acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt ttatggcttt ccgtattctt
    14041 aattgtttaa tttatgtaac atgcaaataa tcaatttgtt aaacacaaca tacaactcat
    14101 taccctgcaa ataattacaa ctggcaaaag ccagagacgg gcttttatgc ccaggagatg
    14161 actcatgatg agcaatgtta agaaaaaaga tgtgccgctg ataagcatca gcctggtggc
    14221 cattcttttc atcgcagctg cattaagcct tttcccacaa caatcggccg acgcggccaa
    14281 cgcaatatac acttttgtta cgcgtacgtt aggttccgcc gtacaggtat tggttttgct
    14341 ggcaatggga ctggtgattt atttagccac cagtaaatac ggcaatattc gtcttggcga
    14401 aggaaaaccg gaatacagca cgctctcctg gctgtttatg tttatttgtg ccggtttagg
    14461 ttcttctacg ctttattggg gggttgctga atgggcctat tattatcaaa cgcctggatt
    14521 aaatatcgca ccgcgttcac aacaggcact cgaatttagc gtcccttact ccttcttcca
    14581 ctggggcatc agcgcctggg caacttatac gctggcctca ttaatcatgg cttatcactt
    14641 tcatgtgcgt aaaaacaaag gtctgagtct ttccggcatt attgccgcga ttaccggcgt
    14701 tcgcccgcaa ggcccatggg gaaaactggt tgatttgatg ttcctgatcg ccactgtcgg
    14761 cgcactgact atttcccttg ttgtcaccgc agcaaccttt actcgtggac tttccgcgct
    14821 gaccggttta cccgataact tcaccgtgca ggcatttgtg atcctgcttt ccggcggcat
    14881 tttttgccta agctcgtgga ttggtatcaa caacggtttg caacgtctga gcaaaatggt
    14941 tggctggggc gcgttcctgc tgccattact ggtgctgatt gtcggcccaa ccgaatttat
    15001 taccaacagc atcatcaatg ccatcggtct gaccacgcaa aacttcctgc aaatgagctt
    15061 attcaccgat ccgcttggcg atggttcatt tacccgcaac tggaccgttt tctactggct
    15121 gtggtggatc tcatacaccc ctggcgtagc aatgtttctc acccgcgttt cccgcggtcg
    15181 taagattaaa gaagttatct ggggactgat cctcggcagc accgtcggtt gctggttctt
    15241 cttcggcgta atggaaagct atgccattca tcagtttatc aatggcgtaa tcaacgtccc
    15301 acaggtgctg gaaacactgg gcggcgaaac agctgtacag caagttctga tgtcgttgcc
    15361 agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg attattttcc ttgcctcgca
    15421 tatggatgcg gtggcctaca ccatggctgc gaccagtacg cgtaatctcc aggaaggtga
    15481 cgatcctgac cgtgggctgc gtcttttctg gtgcgtggtg atcactctga tcccgctttc
    15541 catcttgttt accggtgctt cgctggaaac gatgaaaacc accgtcgtgc tcacagccct
    15601 tcccttcctc gtcattttac tggtgaaagt cggcggattt attcgctggc tgaaacagga
    15661 ttacgccgac attccggctc atcaagttga acattatctc ccgcagacac cggttgaagc
    15721 cctggaaaaa acgccagtgc tccctgcggg aaccgtattc aaaggcgaca actgagcgcc
    15781 atcatcctaa cgataaaggt atccctatga gcaatctgag ccctgacttt gtactacccg
    15841 aaaattattg cgctaacccg caagaggcgt ggaccattcc tgcccgtttt tataccgatc
    15901 agaacgcgtt tgaacacgaa aaagagaacg tcttcgccaa aagctggatt tgcgtcgctc
    15961 acagcagcga actggcgaat gccaatgatt atgtgacgcg tgagatcatt ggcgaaagca
    16021 tcgtgctggt acgcggtcgt gataaggttt tgcgcgcgtt ctataacgtg tgtccgcacc
    16081 gtggtcatca gttgttgagc ggtgaaggaa aagcaaaaaa tgtgattacc tgcccgtatc
    16141 acgcatgggc attcaaactc gatggcaacc tggcccatgc acgtaactgc gaaaacgtcg
    16201 ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa tatgccggat
    16261 tcgtcttcat caacatggac cccaacgcca ccagcgtaga agatcaatta cccggcctgg
    16321 gcgcgaaagt gctggaagcc tgcccggaag tccacgatct gaaactggcg gcccgcttta
    16381 ccacccgcac gcctgccaac tggaagaaca ttgtcgataa ctatctcgag tgctatcact
    16441 gtggtccggc gcatccaggt ttctccgact ccgtacaggt tgatcgttac tggcacacca
    16501 tgcacggtaa ctggacgctg caatacggtt tcgccaaacc gtccgaacag tcgtttaaat
    16561 ttgaagaggg tacggatgcg gcattccacg gtttctggct gtggccgtgc acgatgctga
    16621 acgtcacccc gatcaaaggg atgatgacgg tcatttatga attcccggtg gattctgaaa
    16681 ctaccctgca aaactacgat atttacttca ccaatgaaga gttaaccgac gagcaaaaat
    16741 cgctgattga gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg gttgaaagcg
    16801 tacagaaagg gctgaaatcg cgtggctatc gtggtcaggg gcgcatcatg gccgacagta
    16861 gcggtagtgg catttccgaa catggtatcg cccatttcca taatctgctg gcgcaggtgt
    16921 ttaaggacta atgacatcgg cggcggtatt ttccgccgct gggctgattt ttgatggagt
    16981 acagcaatgt cagactatca aatgtttgaa gtacaggtga gtcaggtgga accgcttacc
    17041 gaacaggtga aacgcttcac gctggtggca accgatggca aaccgctgcc tgcgttcacc
    17101 ggaggaagtc acatcattgt gcagatgagc gatggtgata accagtacag caatgcgtat
    17161 tcactactga gttcgccgca tgacacctct tgttatcaga ttgccgttcg gctggaggaa
    17221 aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa aagtgggcga tcggttaacg
    17281 atttcaacgc ctaataacct gtttgcgcta attccctcag ccagaaagca tctgtttatc
    17341 gcgggcggta ttggtatcac ccctttcctg tcgcacatgg cagagctgca acacagcgac
    17401 gtcgactggc agctacatta ctgctcgcga aatccagaaa gttgcgcatt tcgtgatgag
    17461 ctagtccagc atccgcaggc tgagaaagtt cacttgcatc actcatcaac cggaacacga
    17521 ctggaattag cgcgattatt ggcggatatc gaacctggca cacacgttta tacctgtggt
    17581 cccgaggcgc taaatgaagc ggtaagaagt gaagctgcgc gtctggacat cgtcgccgat
    17641 acgctgcact ttgagcaatt tgctatcgaa gacaaaaccg gcgatgcctt taccctggtg
    17701 cttgcccgtt ccggaaaaga gtttgtggtg ccggaagaga tgactatttt gcaggttatt
    17761 gaaaataata aagccgcgaa agtggaatgt ttatgtcgtg aaggggtatg cggaacctgc
    17821 gaaacagcaa tactggaagg tgaagctgac catcgggatc agtattttag cgatgaagag
    17881 cgtgccagcc agcaaagtat gttgatctgt tgttcgcgtg cgaagggtaa acgcctggtg
    17941 ttggatttgt agtttgccaa agccggatgt ggcgctgagc gcgcccagtc cggcttcgga
    18001 agattttact gcggatattc ctgcaataaa ttgtgtaatg cttccgccat cagctcacca
    18061 cgccagccgg aaatcagctc cggcaaattg ttctgcggtt tcagtttcca gtgccagttc
    18121 agcagttggt tgatttgccg acgcgatgcc agcaattcgg cgctgatctt atgcgtttcg
    18181 ctcacgtcag taatcagcga cttaatcgct ttaaacgctt tacgataacc aggcatgtcc
    18241 atcaggttaa gcatcggctg cggtaaggcc tcttccggca atgtctgcgc tttttccacc
    18301 agcgccagca gcgttttacc gtgaaagcgg atttcgctac cggataaacc caggctgtcc
    18361 agttcgccta aacttcccgg catataacgc gcgaccgacc acaaatgctc ttcacgcacg
    18421 acaaagttca ccgccagatc acgctctcgc gccttgcgca gtcgccagtc ggctaacagt
    18481 tgcagacagg ccagttggcg tgtgcgtaac tgccaggcat tggtgatatc acgccaggca
    18541 tcttccggcg caacgacttc ctgacgacgc atttgcatca ggcggcattc atccagcgcc
    18601 gcaggtagcc agccggaggc ctccgtttct accataagct tggcggtgat cggtaacaga
    18661 taccagacat ccgccgctgc gtattcacac tgacgttcgg tcagcggtct ggccagccag
    18721 tcggtgcgcg attcactctt gtccagcgta acgccggaat actcttccac catggaagcg
    18781 aaaccccatg acatcgggcg tccgcagaag gcagcaagga tttgcgtgtc aatcaagggt
    18841 tgtggtaatt cgccaaagac attgaggaac acttccagat cttcactgcc tgcatggaga
    18901 aattttgtga tggacggatc gcgcaggatc gctttcagcg gtgaccagtc ggtgatcccg
    18961 agaggatcaa ttagcgccag atgctcgcca tcgaaaagtt gaatcaaccc cagctgcggg
    19021 taataagtgc gcgtacgaac aaattcagta tccagggcta tcgccggaaa ggcacggacg
    19081 gcttcacaca aagaagccag cgcatcgtcc gtggtaatca tttggtaatt caaattgttt
    19141 tctctttagt gggcgtcaaa aaaaacgccg gattaaccgg cgtctgacga ctgacttaac
    19201 actcaggctt tattgtccac tttgccgcgc gcttcgtcac gtaattctcg tcgcaaaatt
    19261 tttccgacgt tagatttcgg taactcatca cgaaactcca ccagcttcgg tactttgtag
    19321 cccgtgagct gacggcggca aaaggtcacc agtgactctt cggtaagcga tggatctttt
    19381 ttcactacga agattttcac cgcttcacca ctggagccgg aaggtacgcc aacagccgcg
    19441 acttcctgta cgccaggatg ctgcatgacg acatcttcaa tctcgttggg atagacgtta
    19501 aaaccggaaa ccagaatcat gtctttttta cgatcgacaa tgcgcaggaa cccttcttca
    19561 tccatcaccg cgatgtcgcc ggtgtgtaac cagccatttt tgatgatctc atctgtagca
    19621 tccggacgct gccagtaacc cagcatcacc tgcggtcctt tgacacaaag ctcacccggt
    19681 tgacccggtg gtacttcatt atcatcatca tccaccagtt tggcttccgt cgacggcacc
    19741 ggcaaaccga tgctaccact atgataatca atatcatatg ggttaacgct gaccagcggc
    19801 gcacactcgg taaggccata gccttccagc agatactgtc ctgtcagttt cacccaacgc
    19861 tctgccacca cttgctgcac tggcatccct ccgcctgcgg aaagatgcag actggagaaa
    19921 tccagctgct ggaactcttt attgttcagc aacgcattga acaaggtgtt aacgcccgtg
    19981 atagcggtaa acggatattt cgctaactct tttaccaacc ctggaatatc gcgcgggtta
    20041 gtgataagca ggttctgccc acccagttcg ataaacagca ggcagttaat ggtcagggca
    20101 aaaatgtgat acagcggcag cgccgtcacc accagctctt tgcccggatg caacagcgga
    20161 ccataggtcg cgttaacctg ttccaggttc gccagcatat tgcggtgagt cagcatcgcg
    20221 cctttcgcca caccagtggt gccgccggtg tattgcagaa aagctaaatc ttccggcacc
    20281 agttcgggtt tgacgtactg catccggtag ccgttatgca gtgcgctacg aaatgaaatg
    20341 gcatctggca gatggtattt cggcaccaaa cgcttgatgt atttaacaac gaaattgact
    20401 accgtgcctt ttgccgtaga tagctgatcg cccatacggg tcagaattac gtgctgaacg
    20461 gcggttttat caaccacttt ttccagtgtg tgagcaaagt tagacacgat aacaatcgcc
    20521 gatgcgccgc tatcgttaag ctgatgctca agctcacgcg gggtatacaa cgggttaacg
    20581 tttacgacga tcatccctgc acgcaaaatg ccaaacagcg ccaccggata ttgcaataaa
    20641 ttaggcatca tcaacgcaac gcgatcgcct ttcttcagcc ccaacccttg ttgcaaataa
    20701 gcggcaaacg cgcgactgcg ttcttccagc ttgcggaagg tcattacctc ccccatattc
    20761 acaaacgcag gctgatcggc gtagcgcgcg accgactgct caaacatatc taccagagat
    20821 tgataacggt cagggttgat ctccgtcgga acgtccgcgg gataacggtt aagccaaacc
    20881 ttcttcaatt cttcacctct aaaatgcgtg ttcgtcgtca tcgcaacccc aaatgatata
    20941 catgccgtta acataatatt aactcatcat accagcttga taattaccca acgaaaaggt
    21001 tgcgaagcgc gtcactattt atttttatct ttaccgtaag aatgcagaaa cagcggacca
    21061 gccgctgttt ctttttcttt aaaaacaagc aattattcag ttacaactgt ttgtactctc
    21121 gcgggaccgg gattatacca gccccatccg ccgtagccat agggccagcc acgaccgcca
    21181 taaaaccatg gatcaatcgg ctgaggcggc ataatcacct gctgggttaa atgccaacgt
    21241 ttgtaacccg ttacttgcat caccataaat ttatagggcg tattgccgat tttgccgtca
    21301 accgcaccag tgattggccc aactaccgta accagttgtc cacggaaatc caccgggtcc
    21361 agaaaaccgt tcacatcggc ataaatgcga ccgcgagaag gttctcccag cgtcggtctg
    21421 gctccgctgt ccagcggtac ggtagcaatt tccaggcggg ttttcccttg ctggttttgt
    21481 accgcaacca ctttgccacc aaagcgtgcc tcctgaccaa cgtacagctg cggcgcactc
    21541 atcacccgaa ctaaatcttg ttgcggcgtg ggactgctgc ctttaatggc gtccggcaca
    21601 gtgacacaac cgctcagcat tagcgcaaac gtacctgcca gtatgccttt gataacattc
    21661 ttttgaaccg ccatgatgcg actccttttt ttctcagggc atactcttaa gattcattct
    21721 ttgcccggaa gtttcttcca tgcgacgttg ttacgtaaat aaaccggttc ggcatgttcc
    21781 accgccaccg ttttaccctc ggcaaacatc tgacacgcaa tcggcagcat atcttccgcc
    21841 gctggcagta aaacttctcc gtcgcgcaaa accagcccgc tctctttacc gagatccggc
    21901 caggcttgcc agcccgtccc taccgttacc cattcgccgg aaagctgttg cattcgttca
    21961 tgaacgagtt cgggtttgag tacggcttcg gtttcttcac cgtgccagat accgttttca
    22021 tcacgctgat attcggccca gtaaacttcg cccattcgcg cgtcaatggc tgccagcacg
    22081 cgggttgcgc cgtttttgcg ccacgcccct tgcgccatcg tcattagcgt ggagacgcca
    22141 atcatcggta gctctgcgcc aagtgccagc ccttgcgcaa tgccaatacc aatgcgcacg
    22201 ccggtaaagc taccggggcc gcgcccgtaa gccagagcgt taatatcagt cagggaagtt
    22261 ccgctggtgg tcaggatatc ctgcaccatc ggtaagattc gttgagtatg ttcacgaggg
    22321 caaagctcaa aatgagcgtt gacagtaccg tcgttccaca gggcgacaga gcaggcctct
    22381 gtcgcggtat cgatagccag aattcgcatg ggtcttcgtg cttagatcaa taaaaaggcg
    22441 cgcatcatac catactccgt aacaaattac ctggaggatg gtatcgcaag gaaacgaacc
    22501 gcacgggcaa tgtcacgagt gcgtggcgcg ggcggcagac tggcgagaaa cgtcgcgccg
    22561 taaggacgca tcaccagccg attgtcacaa atcaccagca cgccacgatc gtcggcgtcg
    22621 cgaatcagtc gccctacccc ctgtttgaga gtaatgacgg catctggtag ttgcacttca
    22681 tcgaacgggt cgccaccgcg caaacgacaa tcttccatgc gcgcttttaa cagtggatca
    22741 tccggcgagg taaacggcaa cttgtcgata atcaccaatg acaatgtatc gccacgcacg
    22801 tccacccctt cccagaaact actggttgcc acaagaagcg cgttaccggc gctgacaaat
    22861 tgctgcaaca gttgcccttt gctggtttcc ccctgcaaca aaacgggaag cgtcatggta
    22921 gcgcggaact gctcggccag atcacgcatc atggcgtgcg aggtacaaag cataaaacaa
    22981 cgaccgttgt tagcttcgat gatcggtcgc agcattgccg ctaactggcg agcagaacct
    23041 ggctggttgg tttgcggcag attgcgcggc acacagagta acgcctggcg gctgtaatca
    23101 aacgggcttg gcaatagcaa cgactccgcc tgttctatcc caagccgcga ggtgaaatga
    23161 tgcagatcgt cgttcaccga cagcgttgct gaggtgaaga tccagctacc aggcttttgc
    23221 gccattaact ctttgaattt atccgccacg ctgagcggcg tgagagccag agtaaaatgg
    23281 cgcgaagtgc attcgtacca gtagctgtag cccggctgat tgatctcttt tagccgcttc
    23341 agccgtgtgc gatacaacgt agcgcgctca aatgccgcat ccagcaaggc ggaacgcccc
    23401 agtgacagtt tcgccacgtc ataacaaagt tccagggtgt catcgagcag taaaaatgcc
    23461 cgctgaattt gcggattagc taacagctca cgcaggttac cgcgataacc tggctcaccg
    23521 agttgcagac gaaaatcctg cgcactctgg gcaagacgat cagcgcactt ttgtaactgc
    23581 tgggtgtctt ttaattcggt gcggtaagcg atggtgatgt cttttgccag gtcgagcagt
    23641 tgtcgactgg agagtgactg accaaaatac tggatggcaa tgtccggtag ctggtgggct
    23701 tcgtcgaaga tcatgacgtc cgcttccggg atcaactcgc caaatccact ctctttaacc
    23761 accatatccg ccagaaagag atgatggttt accaccacca catcggcgtc catcgctttt
    23821 ttacgtgctt tgaccacaaa gcaatcttta tacatcgggc agtcgctgcc aagacagttg
    23881 tcgttggtgc tggtaaccag cggccacgcc tgtgaatctt ccgccacgct gacgcaggtg
    23941 ctgatatcac catcgactgt ttgattagac caggagcgca gcaggatcac atcgcttaag
    24001 atttgtaccg gcagatcgcc ccccgccagc gcctgctgtt cgagacgttc gaggcagagg
    24061 tagtttgagc gccctttcag cagcgccacg ttgcccgtat atttcaatgc ctttgagact
    24121 gttggcaaat cgcggctgtg gagctgatcc tgcaacgctt ttgagccggt cgagataatg
    24181 actttctttt tcgcccgcag cgcaggagcc aggtaagcgt aggttttgcc cgtaccggtt
    24241 cctgcttcca ccaccagcgg ctggcctttt tctatcgcct gggtgacggc taccgccatc
    24301 tgtcgctgtg gttctcgcgg cttaaagcct ggtatcgctt tcgccagctg accgtctggt
    24361 gcaaaatcgt ccgtcacact accccctgtt gatttgaaca gggattatgt caggatgagg
    24421 gcgctttcgc cagttgaagt ggtgacggca acctcacatt gtggcagtct ttgcagcacg
    24481 aaaatggaag tataatgagg acaaaatgat gactatcgtt cgtatcgatg ctgaagcccg
    24541 ctggtctgat gtagtaatcc acaacaacac gctctactac actggtgtac cggaaaacct
    24601 cgacgccgat gcttttgagc aaaccgccaa cacgctggcg cagattgacg ccgtgctgga
    24661 aaaacagggc agcaataaat cgagcattct ggatgccacc attttcctgg ctgataaaaa
    24721 cgacttcgcg gcgatgaata aagcgtggga tgcctgggtt gtggcgggtc atgcgccggt
    24781 gcgctgcacg gtgcaagcgg ggctgatgaa cccgaagtat aaagttgaaa ttaagattgt
    24841 ggctgcggtg taagctttat cgaagcaaaa taagtcagac gataatttat cgataatact
    24901 ggtcggtttt acataaatcg accagagatt aagatatt
//
LOCUS       DAZFWP020000070        24273 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFWP020000070 DAZFWP020000000
VERSION     DAZFWP020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.070"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..305
                     /locus_tag="ACHK0Z_RS13050"
                     /old_locus_tag="pgaptmp_002610"
     CDS             6..305
                     /locus_tag="ACHK0Z_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502610.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILALFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(352..1239)
                     /gene="rarD"
                     /locus_tag="ACHK0Z_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             complement(352..1239)
                     /gene="rarD"
                     /locus_tag="ACHK0Z_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU4502611.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1291..1758)
                     /gene="yigI"
                     /locus_tag="ACHK0Z_RS13060"
                     /old_locus_tag="pgaptmp_002612"
     CDS             complement(1291..1758)
                     /gene="yigI"
                     /locus_tag="ACHK0Z_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU4502612.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1923..2792
                     /gene="pldA"
                     /locus_tag="ACHK0Z_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             1923..2792
                     /gene="pldA"
                     /locus_tag="ACHK0Z_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU4502613.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2925..4754
                     /gene="recQ"
                     /locus_tag="ACHK0Z_RS13070"
                     /old_locus_tag="pgaptmp_002614"
     CDS             2925..4754
                     /gene="recQ"
                     /locus_tag="ACHK0Z_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU4502614.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFSEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4818..5438
                     /gene="rhtC"
                     /locus_tag="ACHK0Z_RS13075"
                     /old_locus_tag="pgaptmp_002615"
     CDS             4818..5438
                     /gene="rhtC"
                     /locus_tag="ACHK0Z_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU4502615.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5500..6120)
                     /gene="rhtB"
                     /locus_tag="ACHK0Z_RS13080"
                     /old_locus_tag="pgaptmp_002616"
     CDS             complement(5500..6120)
                     /gene="rhtB"
                     /locus_tag="ACHK0Z_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU4502616.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6231..7253
                     /gene="pldB"
                     /locus_tag="ACHK0Z_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             6231..7253
                     /gene="pldB"
                     /locus_tag="ACHK0Z_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU4502617.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7261..8061
                     /gene="yigL"
                     /locus_tag="ACHK0Z_RS13090"
                     /old_locus_tag="pgaptmp_002618"
     CDS             7261..8061
                     /gene="yigL"
                     /locus_tag="ACHK0Z_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU4502618.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8137..9036
                     /gene="bioP"
                     /locus_tag="ACHK0Z_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             8137..9036
                     /gene="bioP"
                     /locus_tag="ACHK0Z_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU4502619.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8924..9877)
                     /gene="metR"
                     /locus_tag="ACHK0Z_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             complement(8924..9877)
                     /gene="metR"
                     /locus_tag="ACHK0Z_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU4502620.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10000..12261
                     /gene="metE"
                     /locus_tag="ACHK0Z_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             10000..12261
                     /gene="metE"
                     /locus_tag="ACHK0Z_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU4502621.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12300..13115)
                     /gene="ysgA"
                     /locus_tag="ACHK0Z_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             complement(12300..13115)
                     /gene="ysgA"
                     /locus_tag="ACHK0Z_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU4502622.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13377..14138
                     /gene="udp"
                     /locus_tag="ACHK0Z_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             13377..14138
                     /gene="udp"
                     /locus_tag="ACHK0Z_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU4502623.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14279..15706
                     /gene="rmuC"
                     /locus_tag="ACHK0Z_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             14279..15706
                     /gene="rmuC"
                     /locus_tag="ACHK0Z_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU4502624.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15801..16556
                     /gene="ubiE"
                     /locus_tag="ACHK0Z_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             15801..16556
                     /gene="ubiE"
                     /locus_tag="ACHK0Z_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU4502625.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16570..17175
                     /gene="ubiJ"
                     /locus_tag="ACHK0Z_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             16570..17175
                     /gene="ubiJ"
                     /locus_tag="ACHK0Z_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU4502626.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17172..18812
                     /gene="ubiB"
                     /locus_tag="ACHK0Z_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             17172..18812
                     /gene="ubiB"
                     /locus_tag="ACHK0Z_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU4502627.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18891..19160
                     /gene="tatA"
                     /locus_tag="ACHK0Z_RS13140"
                     /old_locus_tag="pgaptmp_002628"
     CDS             18891..19160
                     /gene="tatA"
                     /locus_tag="ACHK0Z_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU4502628.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19164..19679
                     /gene="tatB"
                     /locus_tag="ACHK0Z_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             19164..19679
                     /gene="tatB"
                     /locus_tag="ACHK0Z_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU4502629.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19682..20458
                     /gene="tatC"
                     /locus_tag="ACHK0Z_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             19682..20458
                     /gene="tatC"
                     /locus_tag="ACHK0Z_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU4502630.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20500..21282
                     /gene="tatD"
                     /locus_tag="ACHK0Z_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             20500..21282
                     /gene="tatD"
                     /locus_tag="ACHK0Z_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU4502631.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21279..21767)
                     /gene="rfaH"
                     /locus_tag="ACHK0Z_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             complement(21279..21767)
                     /gene="rfaH"
                     /locus_tag="ACHK0Z_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU4502632.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21934..23427
                     /gene="ubiD"
                     /locus_tag="ACHK0Z_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             21934..23427
                     /gene="ubiD"
                     /locus_tag="ACHK0Z_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU4502633.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23473..24174
                     /gene="fre"
                     /locus_tag="ACHK0Z_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             23473..24174
                     /gene="fre"
                     /locus_tag="ACHK0Z_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU4502634.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
BASE COUNT         5713 a         6046 c         6649 g         5865 t
ORIGIN      
        1 tctatatggg aaatggtaac ttttggcagt tgttcttggg taaagaacct tacagcaaaa
       61 aatctgattt tgttaaggca tggttattgt ggtgggctat taccatcctt gctctattta
      121 ttttaagcct gactacgaca ttaaatttaa gaagtatagg tatagggtta agttttgggt
      181 tttgtcaaat ccttggtaga tatacgatgt ctctaggatg gagtaagtac tggggagttt
      241 tgggggtatt tcctgtaaca aatattgttc tttttcttat cttaattttt ttcaaaaaaa
      301 gataaataag tcagtgggta ggctctttaa acggcctacc catgttaatt cttaactttt
      361 tctacgctgc gtataaatcg catccatcac aaaaattgcc agcgccaccc aaataaaggc
      421 gaaagtcacc atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa
      481 catcagcgtc gggccaatgt actggaaaaa gcctaacgtt gagagacgca agcgcgtggc
      541 agcggcggta aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa
      601 attcagcgac atcgggtttt gccccatatg gctggttgag ctgtcggcaa tagcaaacag
      661 gtaaattgcc gccacgggca gcagccacat ggtttcgatt aacatgccgg tttgcgcttc
      721 aacggcaatc ttcttgcgta ccagaccgta gaaggcaaaa ctaaatgcca gtcccagcgc
      781 gataataggt agcgaaccaa aagtccacag ctggactaac acgccacata tcgccagaat
      841 caccgccagc cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat
      901 gttcaccagc gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac
      961 cgcccagata aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa
     1021 aattttctgt ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat
     1081 cagcaccacc ataaagaaaa acgaccagat cacgcgatgc gtcaggattt catcggcagg
     1141 cacgtagtaa atcaacttga agtacgctgg cgctataccc caaataaaat aagcggcaag
     1201 agcgagtaat acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt
     1261 ggtaacagca gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact
     1321 ggcaatataa agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc
     1381 tgcacgcaac aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc
     1441 aacgcgaaga tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat
     1501 ggtttcgtgg cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc
     1561 cgacgcaatg accccgccgt gcaaaatgct ttgcgcccag ttgcccacca tcattggctg
     1621 atttttaaag gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc
     1681 gcggttaaat ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc
     1741 ggtcagtacg gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat
     1801 gcttctttgt tgttggtttc tactttagga agggataact aacggctatg gagttaagta
     1861 tgtaaaatag cccgcagaaa aatattcacc ttatcaataa ttcgttacgg agaacacgac
     1921 cgatgcggac tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg
     1981 cacaagaggc aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg
     2041 ccaatatgct gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc
     2101 tcatttacac ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg
     2161 aaaatgcgcg taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg
     2221 ggattttagg cccgaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac
     2281 tgtccaatag cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc
     2341 tcggttttgc caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt
     2401 ataaccacga ctctaacggg cgttccgacc cgacctcccg cagctggaac cgcctttata
     2461 ctcgcctgat ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg
     2521 gtaatactga cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg
     2581 gctatcacct cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct
     2641 acggcggcgc ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc
     2701 aggtttacag cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg
     2761 gtgtgggggt tatgctaaac gatttgtttt gatgaacggt tgagtggttg gcaaatctgg
     2821 aatccagcat ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc
     2881 gctgaaaata gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag
     2941 tgttgaatct ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt
     3001 ttcgccccgg ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggtcg
     3061 tcatgcccac cggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg
     3121 gccttaccgt ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactgc
     3181 aagccaacgg cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg
     3241 aagtgatgac aggctgccgc accgggcaaa ttcgtctgct ttatatcgcc ccggaacgcc
     3301 tgatgctgga taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgttg
     3361 atgaagcgca ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg
     3421 gtcagttgcg ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg
     3481 acaccacgcg ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca
     3541 gcagttttga ccgtccgaat attcgctaca tgctgatgga gaagttcaaa ccgctcgatc
     3601 agttgatgcg ctacgtgcag gaacagcgcg gtaagtccgg cattatctac tgcaacagcc
     3661 gggcgaaagt agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct
     3721 atcatgccgg gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg
     3781 acctgcaaat tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc
     3841 gcttcgtggt ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc
     3901 gcgccgggcg tgatggcctg cccgcggaag cgatgctgtt ttacgatccg gccgatatgg
     3961 cgtggctgcg ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgaacgtc
     4021 ataaactcaa tgcgatgggc gcttttgccg aagcgcaaac ttgccgtcgt ctggtactgc
     4081 tgaactattt tagcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc
     4141 cgccgaaaca gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg
     4201 tgaatcagcg ttttggtatg ggctatgtgg tggaagtgat tcgcggcgct aataaccagc
     4261 gtatccgcga ctatggtcat gacaaactaa aagtctacgg tatgggccgt gataaaagtc
     4321 atgaacattg ggtgagcgtg atccgccagt tgattcacct cggcctggtg acgcaaaata
     4381 ttgcccagca ttctgccctc caactgacag aggccgcgcg tccggtgctg cgcggcgaat
     4441 cctctttgca acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat
     4501 cgttcggcgg caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga
     4561 ttgccgatga aagcaatgtc ccgccgtacg tggtgtttaa cgacgcaacg ttgattgaga
     4621 tggctgaaca gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc
     4681 gcaagctgga acgctttggt aaaccgttta tggcgcttat ccgcgcgcat gttgacggcg
     4741 acgacgaaga gtagtcagcg gcataaaaaa gtgccaggat gaagactccg taaacgtttc
     4801 ccccacgagt caaatgtatg ttgatgttat ttctcaccgt cgccatggtg catattgtgg
     4861 cgcttatgag ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc
     4921 gtaaagaagc gatgatgggc gtgctgggca ttacctgcgg tgtaatggtt tgggctggga
     4981 ttgcgttgct tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta
     5041 tggttggcgg tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga
     5101 aaaaagaggc ggtttctgca cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt
     5161 tcctgaaagg tttactgacc aatctcgcta atccgaaagc gattatctac tttggctcgg
     5221 tgttctcatt gtttgtcggt gataacgttg gcactaccgc gcgctggggc atttttgcgc
     5281 tgatcattgt cgaaacgctg gcgtggttta ccgtcgttgc cagcctgttt gccctgccgc
     5341 aaatgcgccg tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat
     5401 ttgccggatt tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta
     5461 agtcggataa ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc
     5521 taacagcgct cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg
     5581 ctttggtcct ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac
     5641 aataatatcg accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg
     5701 ttgcggcatg atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt
     5761 gagattcaca aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga
     5821 tttaaggtca attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc
     5881 cgcgcctgcc cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa
     5941 ccccacgcca accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc
     6001 caccgcgccg cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc
     6061 tggcgacagg cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat
     6121 gatgaactcc cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc
     6181 aggcacgcta aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc
     6241 agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga
     6301 ctgatttctg gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc
     6361 gctttgtccg ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta
     6421 ttgagagcta cgtaaaatat gcggaactgg cctacgattt gttccatttg gggtttgatg
     6481 tcttaatcat cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc
     6541 tcgggcatgt taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcaacagg
     6601 aggtccagcc cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga
     6661 tctccacatt atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc
     6721 caatgtttgg gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg
     6781 ccgaagcgca tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt
     6841 tgccgtttgc tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct
     6901 tctatgctga tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa
     6961 gtattctggc tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc
     7021 tcttgcaggc tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac
     7081 tccgcaccgc cgcgggccat cctgtcgaag gaggacggcc gttggtaatt aaaggtgctt
     7141 accatgagat cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg
     7201 attttttcaa caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt
     7261 atgtaccagg ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta
     7321 tccccttacg ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt
     7381 gcgaccggtc gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct
     7441 tacatgatta cctccaatgg tgcgcgcgtt cacgatctgg atggtaatct gatttttgct
     7501 cataacctgg atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac
     7561 atcattacta acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag
     7621 atgcgctttt ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag
     7681 ccggaaggcg tcagcaaagt gttcttcacc tgcgattccc atgaacaact gctgccgctg
     7741 gagcaggcga ttaacgctcg ttggggagat cgcgtcaacg tcagtttctc taccttaacc
     7801 tgtctggaag tgatggcggg cggcgtttca aaaggccatg cgctggaagc ggtggcgaag
     7861 aaactgggct acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa
     7921 atgctgtcga tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac
     7981 cttcatcccg agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg
     8041 cgtaaactct atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc
     8101 tacaatggat actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg
     8161 attctgtggg ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc
     8221 tattttgcgg tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt
     8281 acccgtggca atagcctgaa aacagtcggc ctgtatatgc tggtgggcgc gatgcagctt
     8341 ggcgtgatgt atatgctgag tttccgcgct tatctctatc tgacggtttc cgaactgctg
     8401 ctgttcaccg tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc
     8461 cgtctgcgct ggggatatgc ttttagtgcc ttgttggcgg tgattggtgc cgggattatt
     8521 cgctatgatc aggtcaccga ccatttctgg actggcttgt tgctggtgca actctccaat
     8581 atcacttttg ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg
     8641 cagcataacg cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg
     8701 ttcttgctgg gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg
     8761 tttcttggcg tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag
     8821 gtggacgccg gaacgctggg aattatgaat aatatgcacg ttccggcagg gctgctggta
     8881 aacctggcta tctggcacca acagccgcac tggccaacgt ttattacagg cgcgctggtg
     8941 atcctggcct cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca
     9001 gatgatcgca ggcgtgattg cgcgctgagc gaataaacgc ttccgtaact ggctgacgct
     9061 gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg
     9121 tcaccaccag accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac
     9181 ccatccgcgc ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg
     9241 ggctgacgcc tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg
     9301 gataaatcaa cagcgtctcg ctggcgagat cttccggtgt aattcgcgtt ttcgccgcca
     9361 gtggatggtc aggtgctaac accagacgca cttcatagtc gaacatcggc gaataatgca
     9421 ggccactgcg cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg
     9481 cgggctgcgg gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct
     9541 tatggaaatt ttctaacgcg ggcgtcagcc actgaataca gctatggcac tcaatggcaa
     9601 tgcgcagacg cgtctgctgc ggttcattgc aggcttgcag tgcctggcta atttgcggca
     9661 gcacctggtt tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc
     9721 tcttacgcac aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca
     9781 gggcggattg cgtctgatgc aacgtcgccg cagcggctgc gagagagccg cagttccgca
     9841 acgcttgtag cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga
     9901 ataatttgcg cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac
     9961 ggctaaatac taaaaattcc ataaggggca tatcatataa tgacaattct taatcacacc
    10021 ctcggtttcc ctcgcgttgg cctgcgtcgc gagctgaaaa aagcgcaaga aagttattgg
    10081 gcggggaact ccacgcgtga agaactgctg acagtagggc gtgaactgcg tgctcgtcac
    10141 tgggatcaac aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt tgcctggtac
    10201 gatcatgttc tgaccaccag tctgctgctg ggtaatgttc cgccacgtca tcagaacaaa
    10261 gacggttcgg tagatatcga caccctgttc cgtattggtc gtggacgtgc gccgactggc
    10321 gaacctgcgg cggcagcgga aatgaccaaa tggtttaaca ccaactatca ctacatggtg
    10381 ccggaattcg ttaaaggcca acagttcaaa ctgacctgga cgcagctgct ggaggaagtg
    10441 gacgaggcgc tggcgctggg ccacaacgtt aagcctgtgc tgctggggcc ggttacttac
    10501 ctgtggctgg ggaaagtgaa aggtgaacag tttgaccgcc ttagcctgct gaacgacatt
    10561 ctgccggttt atcagcaagt gctggcagaa ctggcgaaac gcggcatcga gtgggtacag
    10621 attgatgaac ccgcgctggt actggaacta ccacaggcgt ggctggacgc atacaaaccc
    10681 gcttacgacg cgctccaggg acaggtgaaa ctgctgctga ccacctattt tgaaggcgta
    10741 acgccaaatc tcgacacgat tactgcgctg cctgttcagg gtctgcatgt tgacctcgta
    10801 catggtaaag atgacgttgc tgaactgcac aagcgcctgc cttctgactg gttgctgtct
    10861 gcgggtctga tcaatggtcg taacgtctgg cgcgccgatc ttaccgagaa atatgcgcaa
    10921 attaaggaca ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc gttgctgcac
    10981 agccccatcg acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag ctggtttgcc
    11041 ttcgccctac aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa cagtggtgac
    11101 acggcagctc tggcagagtg gagcgccccg attcaggcac gtcgtcactc tacccgcgta
    11161 cataatccgg cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag ccagcgtgcg
    11221 aatgtctatg aagtgcgtgc cgaagcccag cgtgcgcgtt ttaaactgcc agcgtggccg
    11281 accaccacga ttggttcttt cccgcaaacc acggaaattc gtaccctgcg tctggatttc
    11341 aaaaagggca atctcgacgc caataactac cgcacgggca ttgcggaaca tatcaggcag
    11401 gccattgttg aacaggaacg tttgggactg gatgtgctgg tacatggcga ggccgagcgt
    11461 aatgacatgg tggaatactt tggcgagcac ctcgacgggt ttgtctttac gcaaaacggt
    11521 tgggtacaga gctacggttc ccgctgcgtg aagccaccga ttgttattgg tgacgttagt
    11581 cgcccggcac cgattaccgt ggagtgggcg aagtatgcgc aatcgctgac cgacaaaccg
    11641 gtgaaaggga tgctgacggg gccggtgacc atactctgct ggtcgttccc gcgtgaagat
    11701 gtcagccgtg aaaccatcgc caaacagatt gcgctggcgc tgcgtgatga ggtggccgat
    11761 ctggaagcgg cagggattgg tatcatccag attgacgaac cggcgctgcg cgaaggttta
    11821 ccactgcgtc gtagcgactg ggacgcctat ctccagtggg gcgtagaggc cttccgtatc
    11881 aacgccgccg tggcgaaaga tgacacacaa atccacactc acatgtgtta ttgcgagttc
    11941 aacgacatca tggattccat tgcggcgctg gatgcagacg tcatcaccat cgaaacctcg
    12001 cgttccgaca tggagttgct ggagtcgttt gaagagtttg attatccgaa tgaaatcggt
    12061 ccaggcgtct atgacattca ctcgccaaac gtaccgagcg tggaatggat tgaagccctg
    12121 ctgaagaaag cggcaaaacg cattccggca gagcgcctgt gggtcaaccc agactgtggc
    12181 ctgaaaacgc gcggctggcc agaaacccgc gcggcactgg cgaacatggt gcaggcggcg
    12241 cagaatttgc gtctggggta aatccaaacc gggtggtaat accacccggt cttttctcat
    12301 tacagcgact tcttcccacc atactgctta aaccattcca gcatacgctg ccagccatct
    12361 ttcgcagatt cggcatgata gctcgggcga taatcagcgt tgaatgcatg cccggcatcc
    12421 gggtagacga taatctctgc tttcgcatta gcagcccgca gcgcctggcg catcgtttca
    12481 acgctctcct gcggaatgct gttatcctga ccgccatata agccgagaac cggcgcgtta
    12541 agatcggttg cgatatcaac aggttgtttc ggtgaattca gcgacttatc gcccgtcagt
    12601 ttgccgtacc acgccactgc ggcttttagc tgtggattat gcgcggcata cagccaggtg
    12661 atacgtccac cccagcagaa tccggtgatc attaaacgat gaacatcgcc gccgttgcgg
    12721 gaagcccaac tggcgacatg atcgagatcg gccagcacct gcgagtcagg cacttttgct
    12781 accagaccgc taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag
    12841 taaagttcag gtgcgatagc cagatacccc tccagcgcca gacggcgaca aatgtcgcgg
    12901 atatgttcat gcacgccaaa aatttcctgc actacaatga ccactggcag tgggccatcg
    12961 ctttgctttg gtctggcatg gtaagcaggc atgttatccc cttgtgaagg gatagaggtg
    13021 aagcccgcca caatcgcgtc gtccggggtc tgaacgatgg tcgaagcgag aggcgatgca
    13081 gcaggtgcaa atccagattg ttgtgttgtt gccatggtat tctccgtacc cttataaaaa
    13141 tgttgcgcaa tgttaactat agtcagcatg caacaaatca cattgcctga atcggctcat
    13201 cttttatgca gtcctgcaga atgaagagtg atttatgtga tttgaatcac ttttggtggg
    13261 taaatttatg caacgcattt gcgtcatggt gatgagtatc acgaaaaaat gttaaaccct
    13321 tcggtaaagt gtctttttgc ttcttctgac taaaccgatt cacagaggag ttgtatatgt
    13381 ccaagtctga tgtttttcat ctcggcctca ctaaaaacga tttacaaggg gctacgcttg
    13441 ccatcgtccc tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg
    13501 ttaagctggc atctcaccgc gaattcacta cctggcgtgc agagctggat ggtaaacctg
    13561 ttatcgtctg ctctaccggt atcggcggcc cgtctacctc tattgctgtt gaagagctgg
    13621 cacagctggg cattcgcacc ttcctgcgta tcggtacaac gggcgctatt cagccgcata
    13681 ttaatgtggg tgatgtcctg gttaccacgg cgtctgtccg tctggatggc gcgagcctgc
    13741 acttcgcacc gctggaattc ccggctgtcg ctgatttcga atgtacgact gcgctggttg
    13801 aagctgcgaa atccattggc gcgacaactc acgttggcgt gacagcttct tctgatacct
    13861 tctacccagg ccaggaacgt tacgatactt actctggtcg cgtagttcgt cactttaaag
    13921 gttctatgga agagtggcag gcgatgggcg taatgaacta tgaaatggaa tctgcaaccc
    13981 tgctgaccat gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggt gttatcgtta
    14041 accgcaccca gcaagagatc ccgaatgctg agacgatgaa acaaactgaa agccaagcgg
    14101 tgaaaatcgt ggtggaagcg gcgcgtcgtc tgctgtaatt ctcttctcct gtctgaaggc
    14161 cgacgcgttc ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc tttccaactg
    14221 gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt
    14281 ggatttctca atcatggttt acgcagttat tgcgttggtg ggtgtggcaa ttggctggct
    14341 gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat
    14401 ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa agcgagcact ggcgtgcaga
    14461 gtgcgagtta ctcaataacg aagtgcgcag cctgcaaagt attaacacct ctctggaggc
    14521 cgatctgcgt gaagtaacca cgcggatgga agccgcacag caacatgctg acgataaaat
    14581 tcgccagatg attaacagcg agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg
    14641 tatttttgag cacagcaatc gccgggttga tgagcaaaac cgtcagagtc tgaacagcct
    14701 gttgtcgccg ctacgtgaac aactggacgg tttccgccgt caggttcagg acagcttcgg
    14761 taaagaagca caagaacgcc ataccctgac ccacgaaatt cgcaatctcc agcaactcaa
    14821 cgcgcaaatg gcccaggaag cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac
    14881 ccagggcaac tggggcgagg tagtattgac gcgggtgctg gaggcttccg gtctgcgtga
    14941 agggtatgaa tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc
    15001 ggatgtcatc gtgcggctgc cgcagggaaa agatgtggtg atcgacgcca aaatgacgct
    15061 ggtcgcctat gaacgctatt ttaacgccga agacgactac acccgcgaaa gcgcgctaca
    15121 ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag attatcaaca
    15181 gctgccgggg ctgcgaactc tggattacgt gctgatgttt attcccgttg aacccgcttt
    15241 tttactggcg cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa acaatatcat
    15301 gctggttagc ccgactacgc tgctggtggc gttgcgtact attgccaacc tgtggcgtta
    15361 tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa
    15421 gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa agtctcgaca aagcgcagga
    15481 taactaccgc caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc
    15541 agaagcgttt cgcggtttag gagtagaaat taaacgcgag attaatccgg atttggctga
    15601 acaggcggtg agccaggatg aagagtatcg acttcggtcg gttccggagc agccgaatga
    15661 tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca ttaggggtag
    15721 ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct ggaacaattt
    15781 tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac tttggttttc
    15841 agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat tccgtggcat
    15901 caaaatacga tgtcatgaat gatttgatgt catttggtat tcatcgtttg tggaagcgat
    15961 tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg gctggtggca
    16021 ccggcgacct gacagcgaaa ttctcccgcc tggtcggaga aactggcaaa gtggtccttg
    16081 ctgatatcaa tgaatccatg ctcaaaatgg gacgcgagaa gctgcgtaat atcggtgtga
    16141 ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg gataacacct
    16201 ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat aaagcactgc
    16261 gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc
    16321 caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg ctgccgcgta
    16381 ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa tccatccgta
    16441 tgcatcccga tcaggatacc ctgaaagcca tgatgcagga tgccggattc gaaagtgtcg
    16501 actactacaa tctgacggcg ggggttgtgg cgctgcatcg tggttataag ttctgacagg
    16561 agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct gctcaacacc
    16621 ttcctgtatc gctcacccgc gctgaaaaca gcccgctcgc gtctgctggg taaagtattg
    16681 cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga acgccaggtt
    16741 gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgccta cgccagtgtg
    16801 ttgccgaaac ttcgcgatcg ccagcagctt accgcactga ttcgcagtgg tgagctggaa
    16861 gtgcagggcg atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct ggcagagttc
    16921 gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg aatcagcaaa
    16981 gccatgcgcg gaggcgcaaa gttcctgcat cacggcatta agcgccagca acgttatgtg
    17041 gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt
    17101 gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa
    17161 ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt cgcacttttt
    17221 taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg ctacggctat
    17281 ggcgatactc attattctgg atgccaaatc ggcataaaga caaactttta ggtgagcgac
    17341 tacgactggc cctgcaagaa ctggggccgg tttggatcaa gttcgggcaa atgttatcaa
    17401 cccgccgcga tctttttcca ccgcatattg ccgatcagct ggcgttattg caggacaaag
    17461 ttgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg ggcggcttgc
    17521 cggtagaagc gtggtttgac gattttgaaa tcaagccgct ggcttctgct tctatcgccc
    17581 aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa gtcatccgcc
    17641 cggatatttt gccggttatt aaagcggatc tgaaacttat ctaccgtctg gctcgctggg
    17701 tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg
    17761 aaaagacatt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc
    17821 ggcgcaattt tgaagacagc ccgatgctct acatcccgga agtttaccct gactattgta
    17881 gtgaagggat gatggtgatg gagcgcattt acggcattcc ggtgtctgat gttgcggcgc
    17941 tggagaaaaa cggcaccaac atgaaattgc tggcggaacg cggcgtgcag gtgttcttca
    18001 ctcaggtctt tcgcgacagc tttttccatg ccgatatgca ccctggcaac atcttcgtaa
    18061 gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt gttggctcgc
    18121 taaacaaaga agataaacgc tatctggcag aaaactttat cgccttcttt aatcgcgact
    18181 atcgcaaagt ggcagagcta cacgtcgatt ctggctgggt gccaccagat accaacgttg
    18241 aagagttcga atttgccatt cgtacggtct gtgaacctat ctttgagaaa ccgctggccg
    18301 aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg
    18361 aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa ggggtaggac
    18421 gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg gagtcgtgga
    18481 ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa gcgccgttct
    18541 gggtcgaaaa aatgccagaa ctgcctgaac tggtttacga cagtttgcgc cagggcaagt
    18601 atttacagca cagtgttgat aagattgccc gcgagcttca gtcaaatcat gtacgtcagg
    18661 gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc acattcttgt
    18721 tggtcagccg acctgagtgg gggctgatgc ccggctggtt aatggcaggt ggtctgatcg
    18781 cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg cgggccgtgt
    18841 aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt atgggtggta
    18901 tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt ggcaccaaaa
    18961 agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga
    19021 gcgatgatga accaaagcag gataaaacca gtcaggatgc tgattttact gcgaaaacta
    19081 tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac gcgaagcgcc
    19141 acgataaaga gcaggtgtaa tccgtgtttg atatcggttt tagcgaactg ctattggtgt
    19201 tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag
    19261 cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg
    19321 agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta
    19381 acctgacgcc cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg gagtcgatga
    19441 agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata
    19501 acccggtggt gaaagataat gaagctgcgc atgagggcgt aacgcctgcc gctgcacaaa
    19561 cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg gtaaaacctg
    19621 ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtaaa
    19681 catgtctgta gaagatactc aaccgcttat cacgcatcta attgagctgc gtaagcgtct
    19741 gctgaactgc attatcgcgg tgatcgtgat attcctgtgt ctggtctatt tcgccaatga
    19801 catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt caacgatgat
    19861 cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta tggtgtcgct
    19921 gattctgtca gcgccggtga ttctctatca ggtgtgggca tttatcgccc cagcgctgta
    19981 taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc tgttttatat
    20041 cggcatggcg ttcgcctact ttgtggtctt tccgctggca tttggcttcc ttgccaatac
    20101 cgcgccggaa ggggtacagg tatccaccga catcgccagc tatttaagct tcgttatggc
    20161 gttgtttatg gcgtttggtg tctcctttga agtgcctgtg gcaattgtgc tgctgtgctg
    20221 gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc
    20281 attcgttgtc gggatgctgc tgacaccgcc ggatgtcttc tcgcaaacgc tgttggcgat
    20341 cccgatgtac tgtctgtttg aaatcggtgt cttcttctca cgcttttacg ttggtaaagg
    20401 gcgaaatcgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg aagaataaat
    20461 tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat cggcgttaat
    20521 ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg
    20581 ggagttaatg ggctactcat caccggcact aacctgcgtg aaagccagca ggcgcaaaag
    20641 ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca cgacagcagc
    20701 cagtggcaag ctgcgactga agaagcgatt attgagctgg ccgcgcagcc agaagtggtg
    20761 gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga agagcaggaa
    20821 cgcgcttttg ttgcccagct acgcattgcc gcagatttaa acatgccggt atttatgcac
    20881 tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc cgtggctgga taaactgcct
    20941 ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcgt
    21001 ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg
    21061 gaattgttgc cgttgattcc ggcggagaaa ttgctgatcg aaactgatgc gccgtatctg
    21121 ctcccgcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc ccatctgccc
    21181 catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct ggctgccacc
    21241 acggatgcca atgtcaaaac actgtttggg attgcgtttt agagtttgcg gaactcggtg
    21301 ttcttcacac tgtgcttaat ctctttatta ataagattaa gcaatagcat ggagcgagcc
    21361 tcaccatcgg gttcggtgaa aatggcctga aagccttcga acgcgccttc ggtaataatc
    21421 accttatcac ccggataagg ggttgccgga tcgacaatgt ctttcggttt atataccgat
    21481 agctgatgaa tcaccgccga tgggactatc gctggcgacg cgccaaagcg cacgaagtgg
    21541 ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttctggatc aaattcgaca
    21601 aacaggtagt tgggaaacaa tggctcactg actgcagtac gttttccacg cacgattttt
    21661 tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg ttcctgggca
    21721 cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat gactcttatc
    21781 cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat attccccggt
    21841 ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg caaagatgaa
    21901 cgccgtataa tgggcgtaga ttaagaggct acaatggacg ccatgaaata taacgattta
    21961 cgcgacttcc tgacactgct tgaacagcag ggtgagctaa aacgtatcac gctcccggtg
    22021 gatccgcatc tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg tgggcctgcg
    22081 ctgttgttcg aaaaccctaa aggctactca atgccggtgc tgtgcaacct gttcggtacg
    22141 ccaaagcgcg tggcaatggg catggggcag gaagatgttt cggcgctacg tgaagttggt
    22201 aaattattgg cgtttctgaa agagccggag ccgccaaaag gtttccgcga cctgtttgat
    22261 aaactgccgc agtttaagca agtattgaac atgccgacaa agcggctgcg tggtgcgccc
    22321 tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca atcgcattcc cattatgacc
    22381 tgctggccgg aagatgccgc gccgctgatt acctgggggc tgacagtgac gcgcggcccg
    22441 cataaagagc ggcagaatct gggcatttat cgccagcagc tgattggtaa aaacaaactg
    22501 attatgcgct ggctgtcgca tcgcggcggc gcgctggatt atcaggagtg gtgtgcggcg
    22561 catccgggcg aacgtttccc ggtttctgtg gcgctgggtg ccgatcccgc cacgattctc
    22621 ggtgcagtca cccccgttcc ggatacactt tcagagtatg cgtttgccgg attgctgcgc
    22681 ggtaccaaga ccgaagtggt gaagtgtatc tccaatgacc ttgaagtgcc cgccagtgcg
    22741 gagattgtgc tggaagggta tatcgaacaa ggtgaaacgg cgccggaagg gccgtatggc
    22801 gaccacaccg gttactataa cgaagtcgat agtttccctg tctttactgt gacgcatatt
    22861 acccagcgtg aagatgcgat ttaccattcc acctataccg ggcgtccgcc tgatgaacct
    22921 gcggtactgg gcgtggcgtt gaacgaagtg tttgtgccga ttctgcaaaa acagttcccg
    22981 gaaattgtcg atttttacct gccaccggaa ggctgttctt atcgtctggc ggtagtgacg
    23041 atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta
    23101 cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac
    23161 tggaacgatg tgatttgggc gattaccacc cgtatggacc cggcgcggga tactgttctg
    23221 gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtctccgg gctgggttca
    23281 aaaatggggc tggatgccac gaataaatgg ccgggggaaa cccagcgtga atggggacgt
    23341 cccatcaaaa aagatccaga tgttgtcgcg catattgacg ccatctggga tgaactggct
    23401 atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt atcgatccga
    23461 cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat
    23521 accgtatatc gtgtccgcat cgtgccagat gcggcctttt cttttcgcgc tggtcagtat
    23581 ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcttc gacgccggat
    23641 gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca
    23701 gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca cggagaagcg
    23761 tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctct
    23821 tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt
    23881 tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga ggcgctttcg
    23941 ttgaagcatc ctggtctgca agtggtgccg gtggttgaac aaccggaagc gggctggcgt
    24001 gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc acggtacgct ggcagagcat
    24061 gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgcgatct gttttgcagt
    24121 gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata
    24181 aaaaaacccg cccctgacag gcgggaagaa cggcaactaa aactttattc agtggcattt
    24241 agatctatgt cgcatccggc attcggcaca cga
//
LOCUS       DAZFWP020000071        23421 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFWP020000071 DAZFWP020000000
VERSION     DAZFWP020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.071"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..307)
                     /gene="chbB"
                     /locus_tag="ACHK0Z_RS13175"
                     /old_locus_tag="pgaptmp_002635"
     CDS             complement(<1..307)
                     /gene="chbB"
                     /locus_tag="ACHK0Z_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU4502635.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     "
     gene            complement(606..944)
                     /gene="osmE"
                     /locus_tag="ACHK0Z_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             complement(606..944)
                     /gene="osmE"
                     /locus_tag="ACHK0Z_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU4502636.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1146..1973
                     /gene="nadE"
                     /locus_tag="ACHK0Z_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             1146..1973
                     /gene="nadE"
                     /locus_tag="ACHK0Z_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU4502637.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2203..3090
                     /gene="cho"
                     /locus_tag="ACHK0Z_RS13190"
                     /old_locus_tag="pgaptmp_002638"
     CDS             2203..3090
                     /gene="cho"
                     /locus_tag="ACHK0Z_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU4502638.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3050..3625)
                     /gene="ves"
                     /locus_tag="ACHK0Z_RS13195"
                     /old_locus_tag="pgaptmp_002639"
     CDS             complement(3050..3625)
                     /gene="ves"
                     /locus_tag="ACHK0Z_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU4502639.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFHWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3828..4313)
                     /gene="spy"
                     /locus_tag="ACHK0Z_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             complement(3828..4313)
                     /gene="spy"
                     /locus_tag="ACHK0Z_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU4502640.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4643..5611)
                     /gene="astE"
                     /locus_tag="ACHK0Z_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             complement(4643..5611)
                     /gene="astE"
                     /locus_tag="ACHK0Z_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU4502641.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5604..6947)
                     /gene="astB"
                     /locus_tag="ACHK0Z_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             complement(5604..6947)
                     /gene="astB"
                     /locus_tag="ACHK0Z_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU4502642.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6944..8422)
                     /gene="astD"
                     /locus_tag="ACHK0Z_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             complement(6944..8422)
                     /gene="astD"
                     /locus_tag="ACHK0Z_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU4502643.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8419..9453)
                     /gene="astA"
                     /locus_tag="ACHK0Z_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             complement(8419..9453)
                     /gene="astA"
                     /locus_tag="ACHK0Z_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU4502644.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDLWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9450..10670)
                     /gene="astC"
                     /locus_tag="ACHK0Z_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             complement(9450..10670)
                     /gene="astC"
                     /locus_tag="ACHK0Z_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU4502645.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11116..11922
                     /gene="xthA"
                     /locus_tag="ACHK0Z_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             11116..11922
                     /gene="xthA"
                     /locus_tag="ACHK0Z_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU4502646.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12089..12799
                     /locus_tag="ACHK0Z_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             12089..12799
                     /locus_tag="ACHK0Z_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4502647.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLASEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12804..13481
                     /gene="ydjY"
                     /locus_tag="ACHK0Z_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             12804..13481
                     /gene="ydjY"
                     /locus_tag="ACHK0Z_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU4502648.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDVTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13496..14203
                     /locus_tag="ACHK0Z_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             13496..14203
                     /locus_tag="ACHK0Z_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4502649.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            14203..14751
                     /locus_tag="ACHK0Z_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             14203..14751
                     /locus_tag="ACHK0Z_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4502650.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14761..15927
                     /locus_tag="ACHK0Z_RS13255"
                     /old_locus_tag="pgaptmp_002651"
     CDS             14761..15927
                     /locus_tag="ACHK0Z_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4502651.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            15900..17435
                     /locus_tag="ACHK0Z_RS13260"
                     /old_locus_tag="pgaptmp_002652"
     CDS             15900..17435
                     /locus_tag="ACHK0Z_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4502652.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17435..18088
                     /locus_tag="ACHK0Z_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             17435..18088
                     /locus_tag="ACHK0Z_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4502653.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18155..19462
                     /gene="ynjE"
                     /locus_tag="ACHK0Z_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             18155..19462
                     /gene="ynjE"
                     /locus_tag="ACHK0Z_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU4502654.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19471..20091)
                     /locus_tag="ACHK0Z_RS13275"
                     /old_locus_tag="pgaptmp_002655"
     CDS             complement(19471..20091)
                     /locus_tag="ACHK0Z_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4502655.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20178..20585
                     /gene="nudG"
                     /locus_tag="ACHK0Z_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             20178..20585
                     /gene="nudG"
                     /locus_tag="ACHK0Z_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU4502656.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(20551..20823)
                     /locus_tag="ACHK0Z_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             complement(20551..20823)
                     /locus_tag="ACHK0Z_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU4502657.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            21059..22402
                     /gene="gdhA"
                     /locus_tag="ACHK0Z_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             21059..22402
                     /gene="gdhA"
                     /locus_tag="ACHK0Z_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU4502658.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22519..>23421)
                     /locus_tag="ACHK0Z_RS13295"
                     /old_locus_tag="pgaptmp_002659"
     CDS             complement(22519..>23421)
                     /locus_tag="ACHK0Z_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4502659.1"
                     /translation="TCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAG
                     FKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTF
                     QYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPE
                     QVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIGEEL
                     AWHYIMLASRKAHELFESEEDYQKNSLMGFLYRHICCYRRKLTDAELEACYRYDKQFW
                     EHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         5591 a         6196 c         5978 g         5656 t
ORIGIN      
        1 cttttttaat cgctgcaacc gcagccttaa gcacgcctaa accatcgact ttgccataaa
       61 gcagcgagtc aattacttca accggtttgt tgggtaacaa acgctggatt tcgggcaaca
      121 tataagcaat ctgcggccct aataacacga catcggcatt ctgacctttt tcaccagcca
      181 gtgtttccgg aaatgcttca ataatgaccg gaacttcata tttttctgcc tgtgcgcgca
      241 tttttgatac cagtaaagag gtagacatgc ccgcagaaca aaacagataa atgtgtttct
      301 tttccataaa actgccctca tcgacgatta tctgtcagcc agacactccg caagccttaa
      361 cctgcttcca tgctctgggt aacttgcgaa accaacatga ctttttttgt ggctgaaagg
      421 agtatacggt atagacctga gcaatagtat ttctttgacc ttataaattg tttctccttg
      481 acctgtggtt atgaccctct tcacatttcg ggcaaataat tcgcgaagat aattaagaac
      541 tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg ttactcaagc tttcagacga
      601 attgattact tcgcagcctg tggatcagtg tcgtattcag cacaggtctg ataaccggag
      661 ttgatgacat gtccggtatc atctaacgcg acaaagtagg tttctgcttt accatcacgt
      721 tgacccagga tgtaggtctg gcaagtaccg cgagcatgga tcatgctcac ttcagacgaa
      781 ggtttacccg caatttgtgc aacctgcgcc cggctcatgc cttttttgac gtctttcacc
      841 acaggctgta caaactggtc tttagtacga tcataagccg tacaacccgc cagcatggtt
      901 aataccgccg ctgcactcag aattcctgcc atattcttgt tcatattccg tcctcttgtt
      961 tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc gtgaacgaaa
     1021 cagctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc ccgctgtcgc
     1081 ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct gtctggaggg
     1141 gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg cagattaatg
     1201 ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa acttatccgt
     1261 tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt gccggaaagc
     1321 tgtgccagat ggcgattaat gagctgcgcc aggaaactgg caacgaatca ctgcaattta
     1381 ttgccgtacg cctgccctat ggtgttcagg ccgacgaaca agattgccag gatgccattg
     1441 cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta ttggctagcg
     1501 agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc aatgaaaaag
     1561 cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt gtcgtggtgg
     1621 gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt gacggcggta
     1681 cggatattaa tccgctgtat cgtctcaaca aacgccaggg taaacagttg ctgacggcat
     1741 taggttgccc ggaacacctt tataagaaag ccccaacggc cgatctggaa gatgatcgcc
     1801 cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac gactatctgg
     1861 aagggaaaaa cgtacctgaa caggtcgcca gaacaataga gaactggtat ctgaaaaccg
     1921 aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag taataatttg
     1981 cacctattgg attgtgcgaa aaagagtaat ttgttcacgc cggatgcggc gtgaacgcct
     2041 tattcgacct acaaaactat gcaaattcaa tacattgcag gagtcgaata ggcctgacag
     2101 gcgtagcacg tcagacggtg taacctttgt catcaacccg cctctttttt aatcgcctcc
     2161 cgcctgttac actggataga taaccagcat tcggagtcaa cagtggtacg gcgtttaact
     2221 tctccgcggc tcgaatttga agctgcggca atttatgaat atcccgaaca tttacgttca
     2281 ttccttaatg acttacccac ccgaccaggg gtgtatctgt ttcatggcga aagtgacacc
     2341 atgccgctct atatcggcaa aagcgttaat atccgcagtc gggtgctttc tcatttacgc
     2401 accccggatg aagccgccat gctacggcaa tcccggcgaa ttagctggat ctgcaccgca
     2461 ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca aagaacaaca gccgctgttt
     2521 aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc aattaaatga aaagcgcgtc
     2581 gatgtggtgt atgccaaaga ggtggatttt tcacgagccc ccaacctgtt tggcctgttt
     2641 gccaataggc gcgcagcttt gcaagcattg cagagcatcg ctgatgaaca aaaactttgt
     2701 tatggcctgt tgggactgga accgttaagt cgcggtcgtg catgttttcg ttcagcgcta
     2761 aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg aggaacatgc gctacgcttg
     2821 cgccagtctc tggagcgttt gcgggtggtg tgttggcctt ggcaaggtgc ggtggcactg
     2881 aaagaacagc acccggaaat gactcaatat catattattc aaaactggct gtggctgggg
     2941 gcggttaatt cgctggaaga agcgacaacg ttaattcgga cacccgccgg gtttgatcac
     3001 gacggttata aaattctttg taagccgctg ctttccggta actatgaaat tactgaactt
     3061 gatccggcga atgaccagcg agccagttga tttcactgaa caacagttta ccttgcggtt
     3121 gtaaaagccg cagggtgtgc ctgccatcga accagcacgc gccctggtcg gtagtgagca
     3181 atttatcgcc cagttgccac gccccgttaa tcacaaacac caccccgccg cgtgagccaa
     3241 aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt acagacatcc aggcgtgtca
     3301 tgatgttgaa atccatcgac atctgccctg ccgtcagttt cgcttttacc acctggtccg
     3361 ctgcaaaggc aaaaggctgt aacggtttta aggtatggtt aaagcggtct gcgctttcaa
     3421 ggaacatctc gccgccttcc agcaacgtca ctatcctttc catgccggga aacaaagaaa
     3481 actccccatt cgccgcgatg gatgcaatgc tggcacgcca gtgaaaatca cgttttgccg
     3541 gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt tcgccacaga ttcaccgaca
     3601 ttttacgcat atcaaagtat tccatactcg ctcccggccc gttcttttca ttgttatcgt
     3661 tcaggtaacc gtggatggtt atcggcaact cgggtgattt acgttagtgg tgatcaggct
     3721 ttttttacaa ctgtttgaga agagaaaaga aaaccgccga tcctgtccac cgcattactg
     3781 caaggtagtg gacaagaccg gcggtcttaa gttttttggc tgaaagatta ttcagcagtt
     3841 gcaggcattt taccttttgc cgctggacgt tctgtcagac gcttctcaaa attagcatta
     3901 aattgctttt tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc
     3961 gccagcatgt tagctttgcg ctgttcttcc atttttgcga tctgcgcttc agcttttgct
     4021 ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga
     4081 cgtttcatct ggtcacgctg gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg
     4141 gtcaggttca ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc
     4201 atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta
     4261 gccgcgccaa gagccagggt agaggcaaca aacagtgcag ttaatttacg catattctat
     4321 atccttcctt tcagttattt attacggctt tcttaagtag cgtgccgtgt tgacgagatt
     4381 aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga aagtgtaaaa
     4441 aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg atgagagaat
     4501 aatacaacac gatgattttg cagagattat gaagaactat accggatgac tggtgataaa
     4561 taaagcaaat aaccaggatt aatctgtatt aatttataag aaagcaactt aatacccgca
     4621 gaatgatttc tgcgggtaag tattagctta ttttttcgag cattaatccc gcgcgtaatc
     4681 ccaacgctac caacggatta gggaataaca catactctac atcatgggtt acggtaaaac
     4741 gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt
     4801 cacttgccat atgcatttcg aaggacggcg agtggcgagt aatttgcgaa accacccgat
     4861 aacggagcgg cggtgttctc acgataccga cactctcacc agatagcagc gcagcaattg
     4921 cgctggcagt tactgcaaac tggcgaagat cgttttgccc aaagggcaac gctttgccaa
     4981 gttccagcgt acaggccagc gcgccaaaat gtctggcgct gaaatgggta aacgtaccac
     5041 caggttcctg atggaacacc agcgcctcca gccccgccgc acccagccac gtcagaaatt
     5101 tctcgtccca gggaatgtcg cgttgcggta atacaccgaa ctgcggatgc aaggagccac
     5161 gaattgcggt atgtagatca aggtgccagc gcacagattc tttgcccagg tcataaaaat
     5221 cttccaggca ctgttccagt tcgcgcgccc gacaggtttc tccgctttca gcaaatagct
     5281 gccaacgacc gccaaacatt cgattcatat cgctatggca ataacgtttc ccttgcttca
     5341 gcgcaggagg attcccgagg atcaccagca accgccaacg taacgggatc tcgccgtgag
     5401 atatcgcgcc aagcaacgcg tccagcatct ccacaggtgc cgtctcatta ccgtgtattc
     5461 ccgctgaaat caccagtgcg ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat
     5521 cgcccagcca gcgccagcga acgccgttga tttctcgctc ggtgataacc ggttttttac
     5581 ccgttaaggt cagagcaaga aaattatcca ttgccccctc cctcgcgctg gaacggataa
     5641 accgaaccga gattcagtaa ttggctcaat acatccagtg cttcccgccc tccgcgcagc
     5701 aattgcgggt cggccagatc ggcagcagta aggcgatcgc ggtagtaacg atccacccag
     5761 tcattgagcg cattaaacag cgtatcgttc atcatcaccg ccggattcac cgcccggcgt
     5821 tcttcttctg tcaataccac ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg
     5881 ctttcacgta aatcaaagac ttttagttcg ctaatcgggt tgtcagcggc aaggagttca
     5941 ttgagataac cccatactcc ggcgtgttcc cgacactcct gaggcagcac caacatcatg
     6001 gaaccatcat cgcggctcaa cagttggctg ttaaacagat aggtagacac cgcatccgac
     6061 acggaaacct gagttgccgg aacttctatc gccataaaac cgttcacccg cgcacgcagg
     6121 tttgccagta actgtgactg gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg
     6181 ttactcacgg caatcacgtc attatgaaaa acgccctggt cgataacgtc cgggttttgc
     6241 tgggcgaaaa tcacctgttg gggattcacc tgattcagcc ttgccaccgc ctcgctggct
     6301 tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat cattgccttc ttctcgcccg
     6361 tagacaaaaa gttgcatacc cggctcaccg taatgaccgc cgagacgatt gtggtttgcc
     6421 gccccctcat caccgagcaa cgctacctgc ggcaacgccg aatgaacgct aaatttctct
     6481 tcgtcgttaa aaatcgcttt taaaagtgat tcggtgacgt gcgcttccag cgagcggtga
     6541 aatttattgt tcaggttggc aacggtgaga tgcactttgc catccagcgt atcggcagat
     6601 ggcgcgatcg ttgccgcatt ggctacccac attggcgaag cggaactgac gctggaaagc
     6661 cagtgcggtg cctggcgtgc aactttttcc agtacctgct catcgctacc actgaatccc
     6721 aactgacgca gcaccggaat aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat
     6781 cccgcatcgg caagggcttt cattttcagt aagccctgtt ttgccgcctg tcgcgggtta
     6841 gacacctgaa aacggtgacg ggtagaggct tcattaccaa acgacaggcc agcgtaatga
     6901 tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg cgttcatcgc accacctcat
     6961 cggaaaaatc cagcccgggg ttaagcgttg cgggcaaagt taacgagtcc gactccaggc
     7021 tcgccatagg ccatgcgcag taatctgcgg cataccaggc gctggggcga tggttaccgg
     7081 atgcaccaat gccgccgaat ggcgcggtac tggcagcacc agtaagcggt ttgttccagt
     7141 taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa
     7201 ccagaccgca agagagtccg aagcgagtgt tattcgccat tagaatcgct tcctcgaaag
     7261 aatcataacg ccagacgcgc aataacggtc cgaacacctc ttcatctggt acgccagcaa
     7321 cgcctgtcat ttcaatgatc cccggcgtca gcaacgatgt ctctgattgt aataagcgcg
     7381 gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg ccaggcagta accacctgct
     7441 gtgcggcctg ttcagaaatc agcccgccaa taaacggctg cggttcgtca tcccagttgc
     7501 ccggcgttaa tcgctggctg acggcaacca gacgagcaag aaacgcatcg ccctgcgctc
     7561 cgcttttgag caataaacgg cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg
     7621 actgaatggt cagatggaca gccgcgtcga tatccgccac ctcatcgata attagcggat
     7681 tattaccgcc catctcaagg gcgagaattt tctccggctg accggagagc tggcgatgca
     7741 gctggtagcc ggtattggcg ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac
     7801 tcagcgcctg acccgtttca cgcccgccct gcaccaggtt cagtacgccc ggcggcaagc
     7861 cagcctgctg ccataaacgc attaccgctt cgccactcca cggtgtcagt tcgctgggtt
     7921 taaagataat ggtgttacct gccagcaatg ccggaacgat atgtccgttc ggcaaatgac
     7981 cagggaaatt atacggccca aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg
     8041 ccgcaccgtc cggcatttca ctacgctgct cgccggtacg aacgtgatac gccttaattg
     8101 atatcgcgat tttattgatc atcgccgtca cttcggttgc cgcttcccag cgcggcttac
     8161 ccgtttctct ggcaataatc gcggttaatt cgcctttatt cctttccagc aatccggcaa
     8221 agcgttcgac aacgacctga cgttcagcca atgagagccg cgcccagcgc ggaaacgccg
     8281 cacgggctgc gcgacaagcc tgcccgacct gagcggcatc ggcatcattg ccttgccata
     8341 acacctcgcc cgataccgga ttacgcttca cacgcgatgc gccctggccc gttatccagt
     8401 caccgttaat ccataaagtc atgctgtttt ctcctctgcg cacaggcgca ccagacgaac
     8461 gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc
     8521 ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg tgataatttt cattggcgac
     8581 caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct gccacttcca ccagccgact
     8641 tttacgaatg gcgcgcacgc ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat
     8701 gtcgatatag ttgcggtaac gaaaaccttc tttctccagc accgcgcggg caggcgcggt
     8761 ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg gataaaaagt gggtatagat
     8821 cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc
     8881 ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg ctttgccaga acggtgaata
     8941 gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg
     9001 aaaagccgcc ataaacataa agcgcgattt cgacagcaaa tagccgttgc cctctttgcg
     9061 ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg ccggtgtgat cgttactgag
     9121 aaacagcgtc ggcaatgcgt tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc
     9181 gacgcgatag ttgtaccaga gatcgttcag cccaaccgcc acctcaatgg cacaaatccc
     9241 cgccacggtg cctgtctcgc tatcttccag cacgaacaca tagccctgct cacttttggg
     9301 cagttcgcct tgccaggttt tgattgccct ttcgatacgc gccgaaagcg tggcttcatt
     9361 ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca agctgcatca gcgccgagac
     9421 atctgatcgc tcaacgggac ggatgaccat catgatgaac ctcggctaac aaagtgttcg
     9481 caagcagctg caaagcgatc cagtccggtc gtcacctctt cttcgctgac attgagcgca
     9541 ggcgcaaaac gcaccacgtt gccacccgca atcagtacca tcacgcctgc tttcgccgct
     9601 tcctgagaga tctgtttcgc ttgcccggcg taatcggcat tcagtacaca gccaatcagc
     9661 aaacctaagc cgcgaacttc actgaacaaa ccatagcggt gattaatagt attaagacgc
     9721 tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca tctctggtgt gttgatgagc
     9781 tccagcactt tgcctgccac cgccgaggcc agcggattac cgccataggt ggtgccatga
     9841 gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca acaacgcacc gaccgggaaa
     9901 ccgccgccca gcgctttggc ggtagttaac agatcaggcg tcacgccgta gtgcatatag
     9961 gcatacagtt ccccggtgcg cccgacgccg gtttgtactt catcaaaaat caacagcgca
    10021 ttgtggcggt tacacaattc acgcagacct tgtaaaaacg cgttgctggc tggcaccaca
    10081 ccgccttccc cctggatggg ttcgacaatc accgcacagg tagagtcgtc aatcagcgcg
    10141 ctggcagagt taatatcgtt atatgcagca tgacgaatat ccgccggcag tggcgcaaaa
    10201 tcctgtgaat aggctggctg cccacccgca ctgacagtaa acagcgtgcg accatgaaac
    10261 gcatttttga acgccacgat gccgctctta tggctgccgt agcggtcgtg agcgaattta
    10321 cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg agttacaaaa gaagacgcga
    10381 tcggcaaacg tggcgtcgat caattttttc gccagtcgca gtaccggctc gttggtgtaa
    10441 ccgttgccgg tatgccagaa cttactcgcc tgttcgttca gcgcttcacg cagttccgga
    10501 tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga agtcgatata ctctttcccc
    10561 tgctgatccc acaagcgcga accttcgcca cgtaccggta taaagggtgc cggagcgtaa
    10621 acaggtatca tccattcatc aaagttttca cgcgtaattg gctgagacat agcgacctct
    10681 acagtaaata attcgttatt tatatgttaa caataagtaa tgtttgcgct gtaaatgtag
    10741 attgcagggt tcgtgccagc cagtgataaa agtgcataaa cggcggaggc taactggaaa
    10801 tcaaggagtt ataaccaaac catatgcatt taaagtgcat ataaagtgaa tacgtttgcg
    10861 atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc agaagtaaaa tataattctg
    10921 gaattgtgat cattgacgaa atttactgga aattactgcg ccattctgac gcagcgcgca
    10981 ccaaaagcgg gcattttttg cgccatcgtt gacatcatta acaaccatcg atcaaatcac
    11041 ttaacaacag gcggtaagca acgcgaaatt ctgctaccat ccacgcactc tttatctgaa
    11101 taaatggcag cgactatgaa atttgtctct tttaatatca acggcctgcg cgccagacct
    11161 caccagcttg aagccatcgt cgaaaagcac caaccggatg tgattggcct gcaggagaca
    11221 aaagttcatg acgatatgtt tccgctcgaa gaggtggcga agctcggcta caacgtgttt
    11281 tatcacgggc agaaaggcca ttatggcgtg gcgctgctga ccaaagagac gccgattgcc
    11341 gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc ggcggattat tatggcggaa
    11401 atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc
    11461 cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt atcagaatct gcaaaactac
    11521 ctggaaaccg aactcaaacg tgataatccg gtactgatta tgggcgatat gaatatcagc
    11581 cctacagatc tggatatcgg cattggcgaa gaaaaccgta agcgctggct gcgtaccggt
    11641 aaatgctctt tcctgccgga agagcgcgaa tggatggaca ggctgatgag ctgggggttg
    11701 gtcgatacct tccgccatgc taatccgcaa acagcagatc gtttctcatg gtttgattac
    11761 cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg acctgctgct cgccagccaa
    11821 ccgctggcag aatgttgcgt agaaaccggc atcgactatg aaattcgcag catggaaaaa
    11881 ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct aatttagcag ctctcctggc
    11941 tcaaactggg tcaggagaat taaccttgag aaaaatcaac aaactgtcag taatgatttg
    12001 ttgcctgccg tcctttgtta taccgtcact gcgtttttag ttgtctgacc acttctctat
    12061 tatcaagttt gatataggaa actccacgat gaacgctgag cgtaactttc tgtttgcctg
    12121 tcttattttt gcgctggtca tttacgctat ccacgctttc ggtttattcg atctgctcac
    12181 cgatttaccc cacttacaga cactcattcg ccagagcgga ttgttcggtt atagcctcta
    12241 tattctgtta ttcatcattg ccaccctctt tctgttacca ggaagcatac tggtgatcgc
    12301 tggaggaata gtttttggcc cgctcttagg gacactactc tcattaattg ccgccacgct
    12361 ggcctcctcg agctcattcc tgctggcgcg ctggctggga cgtgatttac tgctgaaata
    12421 cgtgggccat agccatacct tccaggccat tgaaaaaggc attgcgcgta acggtattga
    12481 ttttcttatt ctgacccgct taatcccgtt gtttccttac aatattcaaa attacgctta
    12541 cggattaacc accatcgcct tctggcctta tacccttatt tcggcactca cgaccctgcc
    12601 cggtattgtt atttataccg tgatggcaag cgatctcgcc agtgaaggca ttacgctgcg
    12661 ctttatttta caactctgtc tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa
    12721 actctacgcc cgacacaaac atgtggatct gtctgcttcg cgccgcagcc cacttactca
    12781 cccaaaaaat gaaggataga acgatgtcgc aacattattc agtgtcatgg aaaaaaggac
    12841 tggctgcact ctgcttactg gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg
    12901 cggcggcaaa agtggaatac gacggacttt cgaacagcca accgctgcgc gtcgatgcca
    12961 ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg tttcctcacc gacgtcactc
    13021 gtcacggtat tgtgtttaaa gatggctcca acggacataa atcgctgttt atggcttatg
    13081 cgaccccgaa agcattttat gaagccctga aagaggcagg tggtacgccg ggcgaaaata
    13141 tgacgatgga taataaagaa acgactcttg tcacaggcag caaactggat atttcggtca
    13201 actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt gattgttgac agtaatggca
    13261 agaaactgga catgcgcttt ggcggtaatt taacggcagc agaagagaag aaaacgggtt
    13321 gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag caatgcaaca tacacttatg
    13381 gtgcggtgga aaaacgcggc gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg
    13441 ataacacgct ggcaacggtt acctttaaaa tcaccgaata aagccaggat aaaggatgat
    13501 gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc ctcatcatcc tgttgttcgc
    13561 tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc
    13621 ggcgtttgcc gccgtggacc aacagggtat agaacgcttt attcagtctt acggcgcact
    13681 ggcggcagtt gtctcgttct tgttgatgat tttacaggcc attgctgcac cgctacctgc
    13741 gtttttgatc acctttgcca atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc
    13801 gtggaccagt tcgatggccg gcgcggcgct gtgctttttt atcgccagag tgatgggccg
    13861 cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac agtatggacg gctttttcac
    13921 tcgctacggc aaacacacca tcctggtctg tcggttattg ccttttgtcc ctttcgatcc
    13981 aatcagctat gctgccggtc tgacttcaat acgttttcgc tcgtttttta tcgccaccgg
    14041 gcttggtcaa ttaccggcga ctattgttta ttcctgggcg ggcagcatgt taacaggcgg
    14101 tactttctgg tttgtcaccg gactgtttat tctgtttgcc ctgaccgtgg tgatttttat
    14161 ggcgaagaaa atatggcttg agcgccagaa gaggaatgac tgatgggttt accgccgctt
    14221 agcaaaattc ctttcatttt acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag
    14281 gtgctaagcc cgattcgctg gtgggggcgg atcccgttta tcttttattt ggtgtcaatg
    14341 tttgttgggt ggctggagcg caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc
    14401 agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg tggatatcac cagtatgaaa
    14461 gtcgccgagc gtaccggcag caacgataaa ctgctggcag tggctgactg gcggcaaagc
    14521 ccgctcttta gcgatgaaga acggctggcg ctggagtacg ccgaagccgc aagcgtcaca
    14581 ccgccaacag tcgatgatgc cctgcgtacc cgactggctg cgcattttga cgctcaggcg
    14641 ctcaccgaac tgacggcatt gatcggcctg caaaacctgt cggctcgttt taattctgcc
    14701 atggacattc ccgctcaggg gctgtgccgt attccggaaa aacgttctta aggagggaag
    14761 atgcgccatt gtgggtggtt gctgggattg ttatcgctgt tttctttggc aacacatgcc
    14821 agtgactggc aggaaattaa aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg
    14881 ggcggcgata ccgcaattaa ccgctatctc gactgggtta gcggcgagat gaaaacccat
    14941 tacgctataa acctgaagat tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt
    15001 cagaccgaag ctgctgccgg acgtaaaacg ggcggctcgg tggatctgct ctgggtgaac
    15061 ggcgaaaact tccgcacctt aaaagaggcc aatttattac aaacgggctg ggcggagact
    15121 ctgcccaact ggcgctatgt cgacacacag ctgccggtgc gggaagattt ttcagtgccg
    15181 acacaaggtg cggaatcgcc ctggggcggc gcacaactga cgtttatcgc ccgccgcgat
    15241 gttacgccac agccaccaca aacgccgcaa gccttactgg agtttgctaa agtcaatccc
    15301 ggcacggtta cttatccgcg cccaccggac tttaccggca cggcgtttct tgaacagttg
    15361 ctgattatgc tgacgccaga tcccgccgca ttaaaagaag cgccggacga tgcgactttc
    15421 gcccgtgtca ctgctccctt gtggcaatat cttgatgcgc tacatccgta tttgtggcgc
    15481 gaaggaaagg atttcccgcc ttcgcccgcg cggatggatg ctctgctgaa agccggcaca
    15541 ttgcgcctgt cgctgacctt tagccccgcg catgcgcagc aaaaaatcgc cagcggcgat
    15601 ttgccggcaa gcagttacag ttttggcttt cgcgagggga tgataggcaa cgtgcatttc
    15661 gtcaccattc cagccaacgc gaatgccagt gctgcggcga aggtagttgc caatttcctg
    15721 ctctcacccg atgcgcaact gcgtaaagca gatcccgttg tctggggcga tccttctgtt
    15781 ctcgatccgc aaaaactgcc tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag
    15841 gatctgccgc cggtactggc tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa
    15901 tggttacgcc gttacggtac gcattaatct ttttgctgtg ggcgatggtg gcggtgattt
    15961 atgcaccgct gatcccggca gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact
    16021 ggcaggcgtt atttgccgat ccacagttac cgcatgcatt actggcaacg ctggtgtcga
    16081 caaccatcgc ggcggtcggg gcattgttga ttgccctgct ggtgattgtg gcgctatggc
    16141 ctgggccgaa atggcagcgt atgtgcgccc gtctgccgtg gctgctcgcc attccccatg
    16201 tggcttttgc caccagcgcc ctgctgctct ttgctgacgg agggctgcta tatgactatt
    16261 tcccgtattt cactccgcca atggaccgat ttggcatcgg tctgggcctc acccttgcag
    16321 tgaaagaaag cgcatttctg ctgtggatct tagcggcagt attgagcgaa aaacggctgt
    16381 tgcagcaggt cattgtgctg gattcgctgg gctacagccg ctggcaatgc ctgaactggc
    16441 tgctgttgcc ctccgtcgcg ccttcgctgg caatggcgat gctggcgatt gttgcctggt
    16501 cgctttcggt cgtggatgtg gcgattattc ttgggccagg taatccaccg acgctggcag
    16561 taattagctg gcagtggtta actcagggcg acgcggatca acaaacgaaa ggcgcactcg
    16621 ccagtctgct gctgatgctg ttactcgccg cctacgtttt gctgagctat ctgctatggc
    16681 gcagctggcg gcgcactatt ccccgcgtag atggcattcg caagcctgcc acacctttat
    16741 taccaggcac tacgctggcg agttttttac ccttaaccgg tgtgctgtgt gtggttctgc
    16801 tggcgatcct cgcggatcag tcgacgatca atagtgaagc actcatcaac agcctgacaa
    16861 tggggctggt ggcgacattc atcgctttgc tcctgttact gctgtggctg gaatgggggc
    16921 cacagcgtcg ccagttgtgg ctctggttgc ccattctatt gcccgctctg ccactggtgg
    16981 cgggccagta cacgctggcg ctatggctga acctggatgg aagctggacg gcggtggtct
    17041 gggggcatct gctgtgggta atgccgtgga tgctgtttat cctgcaaccc gcctggcagc
    17101 gcattgattc acggttaatt ttgattgcgc aaacactggg ctggtcgcgg gccaaaatct
    17161 tcttttacgt gaaatgccca ctcatgttgc gccctgtgct gattgccttc gcggtgggat
    17221 tttcagtcag tattgcgcag tatatgccga cgctgtggct gggcgcgggg cgttttccga
    17281 cgctcaccac tgaggcggtg gcattaagca gcggcggcag caacggtatt ctcgccgccc
    17341 aggctttatg gcaactgttg ttaccgctta ttatttttgc cctgaccgcg ttagtcgcaa
    17401 aatgggtagg ttatgtcaga caaggactcc gctaatgctc tgcgtgaaaa atgtttcgct
    17461 acgtttacca gaaagccgct tgctgacaaa cgttaacttt acggtggata aaggtgacat
    17521 tgtcacgtta atggggccgt ctggctgtgg aaaatccact ctgttttcat ggatgattgg
    17581 tgcactggct ggacagtttt cttgtacagg tgagctatgg ttcaatgagc aacggattga
    17641 catgctaccc accgcacagc gtcagattgg cattcttttc caggatgcac tgttatttga
    17701 ccagttcagc gtcgggcaaa atttactgct ggcgctaccg tcgacgctca aggggactgc
    17761 caggcgcaat gccgtgaaag acgcgcttga tcgagcggga ctggctgaaa cttatcacca
    17821 ggaccctgcg acattatcag gcggtcagcg agcgcgggtg gccctgctgc gcgcccttct
    17881 cgctcagcca aaagcgttac tcctggatga gccattcagc cgtcttgatg tggctctgcg
    17941 cgataatttt cgccagtggg tgttcagcga agttcgcgaa ctggcgatcc ccgtcgttca
    18001 ggtaacgcac gatctccagg atgttcctgc tgatagttct gttctggata tggcgcagtg
    18061 gtcagaaaat tacaacaaac tgcgataacg caaagttttt ctcaatgcgt cagttcagaa
    18121 tggcgcactc aaaactacaa tgtcgggatt ttcgatgaaa cgtgtttctc aaatgaccgc
    18181 gctggcaatg gctttagggc tggcttgcgc ttcttcgtgg gccgctgaac tggcgaagcc
    18241 tcttacactt gaccagcttc aacaacaaaa tggcaaagcg atagatactc gccccagcgc
    18301 gttttataac ggctggccac aaaccttaaa tggcccttct ggtcatgaac ctgccgcctt
    18361 aaacctctct gccagctggc tcgacaaaat gagcaccgaa cagctctatg agtggatcaa
    18421 gcaacataat ctgaaaaccg atgctccggt ggcgctgtac ggcaatgaca aagatgtcga
    18481 cgccgtcaaa acgcgtctgc aaaaagcagg ttttacgcat atctccattc tgagtgacgc
    18541 gctaagcgaa ccttcccgtc tacaaaaact gccgcacttt gagcagctgg tttatccgca
    18601 atggctgcac gacctgcaac aaggtaaaga ggttacggcg aaacctgccg gtgactggaa
    18661 agtcattgaa gcggcctggg gcgctcctaa gctttacctt atcagccata ttcctggcgc
    18721 tgactacatc gataccaacg aagtggaaag cgaaccgctg tggaacaaag tttctgatga
    18781 acaactaaaa gcgatgctgg caaaacacgg cattcgccat gacaccacgg tcattctgta
    18841 cgggcgtgac gtatacgctg gagcgcgtgt ggcgcaaatt atgctctacg caggcgtgaa
    18901 agatgtgcgt ctgctggatg gcggctggca aacctggtcc gacgcgggac tgcctgttga
    18961 gcgaggaacg ccaccgaaag tgaaagcgga accggatttc ggcgtgaaga tcccggcaca
    19021 accgcagttg atgctcgata tggaacaggc ccgtggactg ctgcatcgcc aggatgcatc
    19081 gctggtgagc attcgttcgt ggccagaatt tatcggtacg accagcggtt acagctatat
    19141 taaaccaaaa ggtgaaatag ccggagcacg ttggggacac gctggtagcg actcgacgca
    19201 tatggaagat tttcataacc cggatggcac catgcgcagc gccgatgata ttaccgctat
    19261 gtggaaagcg tggaatatca aaccagagca gcaagtttca ttctactgcg gcaccggctg
    19321 gcgcgcgtcc gaaaccttta tgtacgcacg cgccatgggt tggaagaatg tttccgtgta
    19381 tgacggcggc tggtacgaat ggagcagcga tccaaaaaat ccggtagcaa ccggtgaacg
    19441 cggcccggac agtagcaaat aacattgcaa ttactgacgc tggagcgact tcagcgtcag
    19501 atacccactc cacacccgcg taaaggtcgt catccagcac aacgcaccga agatccacgc
    19561 aaaccacgca aaccatgcag ggaataaaca tcccagcaca aacagtaaga tcgtttctgt
    19621 gccttcggtt aatcccccca gatagtaaaa cgacttatgt gcataaccgg ggttatcaat
    19681 ctgatgtttt gctgccagtg cagcaaaggc gagaaaactg ctgcccgtgc cgataaacgc
    19741 aaacaacaac cagccgcctg ccagcgcatt ttgctccggt gcagcaagaa taaagccaaa
    19801 cggcaccagc gcgtaaaaga gaaaatcgag agaaatatca agaaagccgc ccgcgtcggt
    19861 aagctctctg cgccgcgcca gcgcaccgtc cagaccatca agcagcctgt tcaacaaaat
    19921 gacgactagc gccgccagat accagcccag cgccagaaac ggcagagcca gcacgccgat
    19981 ggcaaatccc actaatgtta aaccatctgg cgtaatgccc ggtttatcaa gaacccgcac
    20041 gcactgatgc aacaacggtt taatccgggg atgaagatgg cggtctagca caggcactcc
    20101 ttaaatataa agcctttctg attgagcaac agtgcggata ttatggcatt tttcgcttat
    20161 ctgcccgtgt gtaatttatg aaaatgattg aagttgttgc cgccatcatt gaacgtgatg
    20221 gcaaaatttt actcgcgcaa cgccccgccc agagcgatca ggcgggatta tgggagtttg
    20281 ccggtggtaa agtcgagctg gatgaaagcc agcagcaggc actggtgcgt gagttaaacg
    20341 aagaattggg catcgaagca actgtgggtg agtatgttgc cagccatcag cgagaagttt
    20401 cgggccggat tatccatctt catgcctggc acgtacccga cttccacggg acgttacagg
    20461 cacatgaaca tcaggcgctg gtctggtgct cacctgaaga ggcgctgcaa tatccgctgg
    20521 cccctgctga cattccacta ttagaggcgt ttatggcttt acgcgccgcc agagcagcgg
    20581 attagtgcta agggttttgt catcccgctg gcattgcagc agtattcctt cggctttaat
    20641 taccgcccct tcagaataat tttgatcctg ataaacgcag cactgagtac agggctgcgc
    20701 tgactgcccg cctgaactga acacttctgg cggtacgttt acctccacgt ccggacgata
    20761 atgcgggtta gccagtgcaa ttaatggaaa tgctaatact acggcgaaca atgctcgact
    20821 cacagggaac tccttaacgt tattgtctct gctactgata acggtagccg ggtggcaaaa
    20881 ctttagcgtc tgagttatcg catttggtta tgagattact ctcgttatta atttgctttc
    20941 ctgggtcatt tttttcttgc ttaccgtcac attcttgatg gtatagtcga aaactgcaaa
    21001 agcacatcac ataaacaaca taagcacaat cgtattaata tataagggtt ttatatctat
    21061 ggatcagaca tattctctgg agtcattcct caaccatgtc caaaagcgcg acccgaatca
    21121 aaccgagttc gcgcaagccg ttcgtgaagt aatgaccaca ctctggcctt ttcttgaaca
    21181 aaatccaaaa tatcgccaga tgtcattact ggagcgtctg gttgaaccgg agcgcgtgat
    21241 ccagtttcgc gtggtatggg ttgatgatcg caatcaggta caggtcaacc gtgcatggcg
    21301 tgtgcagttc agctctgcca tcggcccgta caaaggcggt atgcgcttcc atccgtcagt
    21361 taacctctcc attctcaaat tcctcggctt cgaacaaacc ttcaaaaatg ccctgactac
    21421 cctgccgatg ggcggtggta aaggcggtag cgattttgac ccgaaaggta aaagcgaagg
    21481 cgaagtgatg cgtttctgcc aggcgctgat gaccgagctt tatcgtcatc tgggcgcgga
    21541 taccgacgtt ccggcaggtg atatcggcgt tggtggtcgt gaagtcggct ttatggcggg
    21601 gatgatgaaa aagctctcca acaataccgc ctgcgtcttc accggtaagg gcctttcatt
    21661 tggcggtagt cttattcgcc cggaagctac cggctacggt ctggtttatt tcacagaagc
    21721 aatgttaaaa cgccacggta tgggttttga agggatgcgc gtttccgttt ctggctccgg
    21781 caacgtcgcc cagtacgcta tcgaaaaagc gatggaattt ggtgctcgtg tgatcactgc
    21841 gtcagactcc agcggcactg tagttgatga aagcggattc acgaaagaga aactggcacg
    21901 tcttatcgaa atcaaagcca gccgcgatgg tcgagtggca gattacgcca aagaatttgg
    21961 tctggtctat ctcgaaggcc aacagccgtg gtctgtaccg gttgatatcg ccctgccttg
    22021 cgccacccag aacgaactgg atgttgacgc cgcgcatcag cttatcgcca atggcgttaa
    22081 agccgtcgct gaaggggcaa atatgccgac caccatcgaa gcgactgaac tgttccagca
    22141 ggcaggcgta ctgtttgcac cgggtaaagc ggctaatgct ggcggtgtag caacgtcggg
    22201 cctggaaatg gcacaaaacg ctgcgcgcct gggctggaaa gccgagaaag ttgacgcacg
    22261 tttgcatcac atcatgctgg atatccacca tgcctgtgtt gagcatggtg gtgaaggtga
    22321 gcaaaccaac tacgtgcagg gcgcgaacat tgccggtttt gtgaaggttg ccgatgcgat
    22381 gctggcgcag ggtgtgattt aagttgtaaa tgcctgatgg cgctacgctt atcaggcata
    22441 caaatgggca caattcattg cagttacgct ctaatgtagg ccgggcaagc gcagcgcccc
    22501 cggcaaaatt tcaggcgttt atgagtattt aacggatgat gctccccagg gaacatttct
    22561 tatgggccaa cggcatttct tactgtagtg ctcccaaaac tgcttgtcgt aacgataaca
    22621 cgcttcaagt tcagcatccg ttaactttct gcgatagcag cagatatgcc tgtaaagaaa
    22681 tcccattaga ctattttttt gataatcttc ttcgctttcg aacaactcgt gcgcctttcg
    22741 agaagcaagc attatataat gccaggccag ttcttctcca attgtcccgt tttgaaaagc
    22801 tgtgcttgat atcgagatca tccatgataa ttccgccgcc catattagct tcgccgagga
    22861 tttaccggag cgattattag cgcaatcaga gatatagtct gagggaaaaa cagcaaattt
    22921 attcaacaag gcgataacct gctctggggc ttcctccatg tttgctttaa aggtattggc
    22981 tccatggtcg ccagaaagaa aatgctccat taaggcacaa taactttcgc tatcttcgat
    23041 accccattga tcctctaaag actcgcgtct tttacttatg atatcgatcg agtcaaaagg
    23101 aagcacatga tattggaagg tatctttgcc aggttcaggc tttcgcggcc agaactccag
    23161 cgtttcagac cattgcttat gatagaatcg ataaggtgcg atcaattgta gcgcctgtaa
    23221 cttctcgata ctgagcggct caataccttt agcctgataa taatgcagtt gttctttttt
    23281 tgctttaaaa ccggcccgaa caataagccc catcataatt aatagataaa gaaaagagca
    23341 tcccgcagta atcaggcctc tttcattcaa accgttggat gttatcgctg cgaatacaaa
    23401 cattacagcg acaacacatg t
//
LOCUS       DAZFWP020000072        23389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFWP020000072 DAZFWP020000000
VERSION     DAZFWP020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.072"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            161..703
                     /locus_tag="ACHK0Z_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             161..703
                     /locus_tag="ACHK0Z_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4502660.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            786..1487
                     /locus_tag="ACHK0Z_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             786..1487
                     /locus_tag="ACHK0Z_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4502661.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1512..4112
                     /locus_tag="ACHK0Z_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             1512..4112
                     /locus_tag="ACHK0Z_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4502662.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            4103..5173
                     /locus_tag="ACHK0Z_RS13315"
                     /old_locus_tag="pgaptmp_002663"
     CDS             4103..5173
                     /locus_tag="ACHK0Z_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4502663.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5185..5727
                     /locus_tag="ACHK0Z_RS13320"
                     /old_locus_tag="pgaptmp_002664"
     CDS             5185..5727
                     /locus_tag="ACHK0Z_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4502664.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5735..6250
                     /locus_tag="ACHK0Z_RS13325"
                     /old_locus_tag="pgaptmp_002665"
     CDS             5735..6250
                     /locus_tag="ACHK0Z_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4502665.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6216..6953
                     /locus_tag="ACHK0Z_RS13330"
                     /old_locus_tag="pgaptmp_002666"
     CDS             6216..6953
                     /locus_tag="ACHK0Z_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4502666.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            7064..8074
                     /gene="pyrD"
                     /locus_tag="ACHK0Z_RS13335"
                     /old_locus_tag="pgaptmp_002667"
     CDS             7064..8074
                     /gene="pyrD"
                     /locus_tag="ACHK0Z_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU4502667.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8248..8790
                     /gene="zapC"
                     /locus_tag="ACHK0Z_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             8248..8790
                     /gene="zapC"
                     /locus_tag="ACHK0Z_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU4502668.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8787..9896)
                     /gene="ycbX"
                     /locus_tag="ACHK0Z_RS13345"
                     /old_locus_tag="pgaptmp_002669"
     CDS             complement(8787..9896)
                     /gene="ycbX"
                     /locus_tag="ACHK0Z_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU4502669.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10140..12248
                     /gene="rlmKL"
                     /locus_tag="ACHK0Z_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             10140..12248
                     /gene="rlmKL"
                     /locus_tag="ACHK0Z_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU4502670.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12259..14166
                     /locus_tag="ACHK0Z_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             12259..14166
                     /locus_tag="ACHK0Z_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4502671.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQSVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14296..15549
                     /gene="pqiA"
                     /locus_tag="ACHK0Z_RS13360"
                     /old_locus_tag="pgaptmp_002672"
     CDS             14296..15549
                     /gene="pqiA"
                     /locus_tag="ACHK0Z_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU4502672.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VADLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15554..17194
                     /gene="pqiB"
                     /locus_tag="ACHK0Z_RS13365"
                     /old_locus_tag="pgaptmp_002673"
     CDS             15554..17194
                     /gene="pqiB"
                     /locus_tag="ACHK0Z_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU4502673.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17191..17754
                     /gene="pqiC"
                     /locus_tag="ACHK0Z_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             17191..17754
                     /gene="pqiC"
                     /locus_tag="ACHK0Z_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU4502674.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQITVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            18010..18177
                     /gene="rmf"
                     /locus_tag="ACHK0Z_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             18010..18177
                     /gene="rmf"
                     /locus_tag="ACHK0Z_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU4502675.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18247..18765)
                     /gene="fabA"
                     /locus_tag="ACHK0Z_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             complement(18247..18765)
                     /gene="fabA"
                     /locus_tag="ACHK0Z_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU4502676.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18834..20594)
                     /locus_tag="ACHK0Z_RS13385"
                     /old_locus_tag="pgaptmp_002677"
     CDS             complement(18834..20594)
                     /locus_tag="ACHK0Z_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4502677.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20780..21232
                     /gene="matP"
                     /locus_tag="ACHK0Z_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             20780..21232
                     /gene="matP"
                     /locus_tag="ACHK0Z_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU4502678.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21308..22348)
                     /gene="ompA"
                     /locus_tag="ACHK0Z_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             complement(21308..22348)
                     /gene="ompA"
                     /locus_tag="ACHK0Z_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU4502679.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22705..23214)
                     /gene="sulA"
                     /locus_tag="ACHK0Z_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             complement(22705..23214)
                     /gene="sulA"
                     /locus_tag="ACHK0Z_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU4502680.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
BASE COUNT         6005 a         5470 c         6095 g         5819 t
ORIGIN      
        1 ttcagttaat atttaaatat tcatgaaatc tataaattaa agatttgtca cttattggat
       61 ttagtatgct tgatttaaat ggcggtcaat tattgttatt attaagtcta atgtcattta
      121 aaggcattat catatttacg aagagatcag gatgatgacg atgaaaaaaa gtgtattgac
      181 ggcgtttata actgtggtat gtgcaacgtc cagcgttatg gcagctgatg ataatgctat
      241 cacggatggc aaagtaacat ttaatggtaa agttattgct ccagcttgta ccctggtagc
      301 tgcgacgaaa gattcagtag tcacattgcc aaatgtcagc gccaccaagt tgcaaactaa
      361 tggagcagtc tctggcgtta aaactgatgt accaattgcc ttagaaggct gtgatgttac
      421 tgtaactaaa aatgcgacgt ttacctttag tggtacggct gacggcgtgc aacctacagc
      481 atttgccaac caggctacca ccgatgcagc gacgaatgtt gctctacaaa tgtatctgcc
      541 agatggttct acatcggtaa ctcccggtac cgaaacgtcg aatattcagt tagcggattc
      601 tgctgagcaa acagtgacct ttaaagttga ttatatcgct acggggaaag cgacttctgg
      661 taatgtgaat gcggtaacaa atttccatat taactattat taatagaact cattaattgt
      721 tttattaatt agtacccctc tagtgttctg gaggggatat tcatattttt taagagtgac
      781 tatttatgaa aacttgcata acaaagggaa ttgtgaccgt aagtttaacg gcaatattac
      841 tcagctgctc atccgcatgg gcggcaggta aaggcgggat tggacttgca gcaacacgtc
      901 ttgtttattc agaaggtgaa gagcaaattt cactgggtgt acgtaacacc agtccggatg
      961 ttccttatct tattcagtca tgggtgatga ccccagataa taaaaaatca gcagatttta
     1021 ttattacacc accgctattt gtgctgaatc cggctaatga gaatctgtta cgcattatgt
     1081 acattggtgc gccgttggcg aaagacagag aaaccctttt cttcactagc gtacgggcag
     1141 tcccttcaac aacgaagcgg gaagagggaa ataccctgaa gattgccaca caaagcgtca
     1201 tcaaactttt ctggcgacca aaaggtttag cgtatccctt aggcgaggct ccggcgaaac
     1261 tgcgttgcac ttcgtcagct gacatggtta cggtcagtaa cccaacacct tatttcatta
     1321 ccctgacaga cctgaaaata ggtggaaaag tagttaaaaa tcaaatgatt tccccctttg
     1381 ataaatacca attttctctg ccaaaggggg ccaaaaatag cagcgtaacg tatcgaacca
     1441 tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc gtcttcagtt
     1501 aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct ggtggagtgc
     1561 catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca caagctgaaa
     1621 cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat ttgtctcgtt
     1681 tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt ctgaatcaga
     1741 caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag gggattgccg
     1801 cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg tttccggctt
     1861 ttaaacaact ggacaaacaa gcgtgtgtgc cattggcgga gattattccg gatgccagcg
     1921 taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc gccatcaaaa
     1981 gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac gcgctattac
     2041 tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt gattctggcg
     2101 acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga ttgcgcaaca
     2161 attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc agcagctatt
     2221 tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat tatactgcag
     2281 gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat gacaacatgc
     2341 tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa agcaatgccc
     2401 agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg cctggtgctt
     2461 ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt gaaatcaaag
     2521 aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca ttactccagc
     2581 gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat agtaatgaac
     2641 agcaggagag caaatttgcc caggccacgt tgcagtgggg cggaccgtgg ggaacgacat
     2701 ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt ctgggattta
     2761 accttggcga tttcggagca atttcgttcg atgtgaccca ggcgaagagt acgctggcag
     2821 accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg ctcaaccaat
     2881 tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc tacacccttt
     2941 ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat gatgaagata
     3001 cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa ctgcaggtca
     3061 atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc cagcaaactt
     3121 actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg caaattaaag
     3181 atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct gatgctgatc
     3241 aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga agtaatgata
     3301 gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc gataacgaag
     3361 ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt aacctgagct
     3421 acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc gccagcatgg
     3481 actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat aacggcagtc
     3541 aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag ggcattaccc
     3601 tggggcaatc gctgggggaa actaacgtcc tgattgcagc accaggcgca gagaatactc
     3661 gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt gtcccctatg
     3721 ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa cgtaacgtgg
     3781 atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt ctggcggagt
     3841 tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt ataccgctgc
     3901 gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt attgatgatg
     3961 atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta cgctggggcg
     4021 aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa attaactctg
     4081 cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg tgttattact
     4141 acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg cttatagcgg
     4201 gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg ttaaaaccct
     4261 ggaggaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt ggaacgaatc
     4321 gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg gccgatgggc
     4381 attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa ttaatgacta
     4441 tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg ttcctttcga
     4501 aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg gtcaacgttt
     4561 aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat tgcagccgtt
     4621 cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat gctacaacat
     4681 acccgcaggt gattcctgca cgactacagg tacaccggtt ttagtgtatt acctgtctgg
     4741 tactatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg aggtcgatct
     4801 gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg gggccagaac
     4861 ggcagaactt gcaattccag tcaggtgtaa cacgggaaac gcggggttag ttaacgtcaa
     4921 cctgagtctg acggcaacca cagaccccag ctatccccag gcgattaaga cgtcacgtcc
     4981 tggcgtgggc gtggtggtga ccgatagcca gaacaacatt atttcccctg ctggtggaac
     5041 attaccgctc tctattcctg atgatgcaga cagtatcgcg cgaatgaatg tctatccagt
     5101 cagcacgaca ggtgtaccac cagaaaccgg gcgatttgaa gccacggcaa cggtgagaat
     5161 aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatttcagtg cgttatcttg
     5221 ttctttagca ttcttaacat ccatgtcggg atggctgggc ctgaacaagt tagtatgcat
     5281 atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc attacaaaat
     5341 attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt aagcactgct
     5401 gctgatttga atatcgatat caccggttgt gccgctggta ttactggcgc ggacgtcctt
     5461 tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga cacaggcgga
     5521 agcggtggta tggcaacggg gattgccgtg caaattcttg atgcgcaaag tcagcaagaa
     5581 atcccgctca atcaggtcca gcctcttacg cccttaaaag ccggggataa cacactcaaa
     5641 tatcaacttc gttataagtc cacaaaggcg ggagcaacgg gcggtaatgc gacggcggtt
     5701 ctctattttg atctggttta ccagtgaagg agtgatgttg aaacggataa tctggattct
     5761 gttcttactg ggattaacgg ggggctgtga gctatttgcc catgatggca cagtcaacat
     5821 tagcggatcg tttcgccgta atacatgcgt gctggcacag gatagcaagc aaatcaatgt
     5881 gcagttaggc gatgtctcgc tgacacgttt ttttcatggg aattatggcc cggagaaatc
     5941 tttcatcatt aatttgcagg attgcggaac agatgtcagc acggttgacg tcaccttttc
     6001 aggaaccccc gacggagtgc agagcgaaat gctgtccatt gaaagtggta cggatgcagc
     6061 cagcgggctg gcaattgcga ttctggatga tgcgaagata ttgattccgc ttaaccaggc
     6121 gagtaaagac tacagcctac atagcggtaa ggtgccatta actttttatg cgcaattgcg
     6181 acctgtcaat agcgatgtgc agtcgggtaa agtgaatgcc agcgcgacat ttgtacttca
     6241 ttatgactaa tacgtggaac agattggcgc tcttgatttt cgccgtttta tcgctgctgg
     6301 tggcgggtga attgcgggca ggagtggtgg tcggcggaac gcgatttatc tttccggcag
     6361 acagagaatc gatatctatt ttactgacta ataccagtca ggaatcctgg cttattaaca
     6421 gtaaaatcaa ccgcccaacg cgttgggccg ggggggaagc gtcgacagtg ccagcaccat
     6481 tactggccgc tccgccactt attctcctga agcccggtac tacaggcacg ttgcgcttgc
     6541 tgagaacgga aagcgacatc ttgcctgtgg atcgcgaaac gctatttgag ttaagcattg
     6601 ccagcgtgcc atccggcaaa gttgaaaatc agagcgtaaa agtggcgatg cgctcggtat
     6661 ttaaactgtt ctggcgaccc gaaggtttgc cgggcgaccc gctggaagct tatcaacaat
     6721 tacgctggac acggaattcg cagggtgtac aactcactaa cccaacgcct tactacatta
     6781 acctgattca gttgagcgta aatggtaaag cgttaagtaa tgcgggagtt gtgccgccta
     6841 aaagccagcg tcagacaagc tggtgtcagg caatcgcacc gtgtcatgtc gcctggcggg
     6901 cgataaatga ttacggcggg ttgagtgcaa agaaggagca aaatctgccc tgaaacaggt
     6961 tcggaaaacg tttgcgtttt ttttgccgca ggtcaattcc cttttggtcc gaactcgcac
     7021 ataatacgcc cccggtttgc acaccgggaa tccaggagag ttcatgtact accccttcgt
     7081 tcgtaaagcc cttttccagc tcgatccaga gcgcgctcat gagtttactt ttcagcaatt
     7141 acgccgtatt acaggaacgc cgtttgaagc actggtgcgg cagaaagtgc ctgcgaaacc
     7201 tgttaactgc atgggcctga cgtttaaaaa tccgcttggt ctggcagccg gtcttgataa
     7261 agacggggag tgcattgacg cgttaggcgc gatgggattt ggatcgatcg agatcggtac
     7321 cgtcacgcca cgtccacagc caggtaatga caagccgcgt ctctttcgtc tggtagatgc
     7381 cgaaggtttg atcaaccgta tgggctttaa taatcttggc gttgataacc tcgtagagaa
     7441 cgtaaaaaag gcccattatg acggcgtcct gggtattaac atcggcaaaa ataaagatac
     7501 gccagtggag cagggcaaag atgactatct gatttgtatg gaaaaaatct atgcctatgc
     7561 gggatatatc gccatcaata tttcatcgcc gaatacccca ggattacgca cgctgcaata
     7621 tggtgaagcg ctggatgatc tcttaaccgc gattaaaaat aagcaaaatg atttgcaagc
     7681 gatgcaccat aaatatgtgc cgatcgcagt gaagatcgcg ccggatcttt ctgaagaaga
     7741 attgatccag gttgccgata gtttagttcg ccataatatt gatggcgtta ttgcaaccaa
     7801 taccacactc gatcgttctc ttgttcaggg aatgaaaaat tgcgatcaaa ccggtggctt
     7861 aagtggtcgt ccgcttcagt taaaaagcac cgaaattatt cgccgcttgt cactggaatt
     7921 aaacggtcgc ttaccgatca tcggtgttgg cggcatcgac tcggttatcg ctgcgcgtga
     7981 aaagattgct gcgggtgcct cactggtgca aatttattct ggttttattt ttaaaggtcc
     8041 gccgctgatt aaagaaatcg ttacccatat ctaattattt cttcgaattc ctctttataa
     8101 gataatcagg gctttatttt cagccctggt tgttttatat tcatcgctgt tgcttattta
     8161 gacattttgt acttttattg ttgaggttat taagcgaagc gacaatggat tgtgttgctg
     8221 cggttttata gggtagggga gaggcagatg cgaattaaac cagacgataa ctggcgttgg
     8281 tattacgatg aagagcacga tcgaatgatg ctcgatttag ccaatggtat gctatttcgc
     8341 tcacgttttg cgcgcaagat gttgactcca gatgcctttt ctcccgcagg cttttgcgtt
     8401 gacgacgccg cgctctattt ctcttttgaa gaaaagtgcc gcgattttaa tttatctaaa
     8461 gaacaaaaag ccgaactggt gctgaatgca ctggtagcaa ttcgctatct caaaccgcaa
     8521 atgccgaaaa gctggcattt tgtttcccat ggtgaaatgt gggtgcccat gccgggcgat
     8581 gcggcctgcg tttggttgag cgatactcac gagcaggtca atttgctggt cgttgaatct
     8641 ggcgaaaatg ccgcactatg cctgctggca caaccctgcg ttgttattgc gggtcgcgcg
     8701 atgcagttgg gcgacgccat taaaatcatg aacgacaggc tgaaaccgca ggttaatgtt
     8761 gacagcttca gcctcgaaca ggcagtctaa cgcgccaact taagtgcagt cttcggtaca
     8821 cagctacagc aaagaatggt gccatcatcg cccattgctg atttttttag cggcgtgact
     8881 tcgccttcta aaagctgaac acggcaactt ccacaaatgc ccgcgcggca agaataaggg
     8941 atacgaattc cctgattttc taattgctcc agcaacacct gttggttatt tccacgaaat
     9001 gcctgtccct gccagtcaat atctacattt gcgtccggtt gttgcgtgat gttggcggta
     9061 tcatcagcgg cagctgcgcc gtaaattttt gccggagccg ttgccagaat ttccacctca
     9121 tcgccaacgc gaatcacgcc gctattacgg gcaattaaat tctgaccaaa atcgacatcg
     9181 ccgttatcct gggcagtgcg gaaagattgc aatgttttta atggttcgcc tgccggatgt
     9241 ttttgccctt tttctgggct gacggtggtg aaaatacagc ggctacaagg tttaaccaca
     9301 tcaaacacca catcaccaat gcgaatcact ttccagctat cttcttccca ggctgacgcg
     9361 ccggaaacca ccagattggg gcggaattgc tccattttta cactggccgg acaacgttgt
     9421 tggagatcac gtaacgaggc ttcgttagca agaaggtaag gatagccatc agcaaatgac
     9481 aggggtacag tgttgtggcg tttcacgcgc cgggtcattt gtggccccac ccagcgtaat
     9541 tgcacttcgc gggagaaaaa tccacttagc catttgttga tcgcgtctgg cgcaattcgc
     9601 gcggtaaaat gtgtgcccca aacttcggtt ggtgcgtctt gtgtggcgaa atcagcaaaa
     9661 cgaacatatg cgctactgcc atctggtgcg gtgagatgca agccatcatg cacaggtgaa
     9721 ggagtaaacc gtaccatctg gggaaactgg cgagcggtaa taaacgtacc gtcaggttcc
     9781 gtgatcataa agatgcgatc gaaggccaga ccactgacat cagccagagc atgtgtaaga
     9841 ccaatgccgc gcatcgattt aacaggatga ataaaaagcc gggttaatgt caccacggtg
     9901 cggtcctcaa atgaaaataa gccctcaact ttatgacatg acgcgcttat tagctataat
     9961 gcgcaacaat tttcttagcg cctgtcccat taggctattt tatttgtcag tttggccctg
    10021 ggttgtgctc gaaatcctca cgtacttgtg tacgctccgg tttctccgcg ctgtccatgt
    10081 ccagactgac tgcaacaatt acgcctactg cggtaggctc ttagagtaaa agtgacgata
    10141 tgaattctct gtttgccagt acggcccgtg ggctggaaga gctgttaaaa actgaactgg
    10201 aaaacctggg ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc aagggcgaca
    10261 cacggcttgt ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt atgttgccgc
    10321 tgggcgagtg taaggtttac agcgatttag acctctatct cggtgttcag gcgatcaact
    10381 ggacagagat gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt ttgaatgaca
    10441 ccatccgcaa cagtcagtac ggcgcgatga aagtgaaaga cgcgatcgtc gatgctttca
    10501 cgcggaaaaa tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc cgcgttaacg
    10561 tctggctgca taaagaaacc gccagtatcg ctcttgatct gagtggtgat ggtttacatc
    10621 tgcgtggcta tcgcgatcgt gctggtattg cgccgatcaa agaaaccctg gcagccgcta
    10681 ttgtgatgcg atccggctgg cagccaggaa caccgctgct cgatccgatg tgtggttccg
    10741 gtacgttgct gattgaagcg gcgatgctag cgaccgatcg cgcaccaggc ttgcaccgtg
    10801 ggcgttgggg ctttagcggc tgggtgcagc atgatgaaga tatctggcag gaagtgaaag
    10861 cggaagcgca aactcgtgcc cgtaaaggcc tggctgagta tagctctcat ttttacggtt
    10921 cggacagcga cgcacgggtg attcaacgtg cgcgcactaa cgcccgtctt gcggggattg
    10981 gtgaactgat cacctttgag gtgaaagatg tcgcgcaact gaccaatccg ctgccgaaag
    11041 ggccgtacgg cacagtgttg agcaacccgc catacgggga acgtctggac agcgaaccgg
    11101 cgctgattgc gctgcatagc ctgctgggtc ggatcatgaa aaaccagttt ggtggctgga
    11161 atctctcttt gtttagtgcc tcgccggatc tgctaagctg cttgcagctg cgtgcagaca
    11221 aacagtacaa ggcgaaaaac ggcccgctgg actgcgtaca gaaaaattac cacgttgccg
    11281 aaagcactcc agacagcaaa ccggcgatgg tagcggaaga ctacgccaac cgcctgcgta
    11341 agaacctcaa aaaattcgag aagtgggctc gccaggaagg aattgaatgt taccgcctgt
    11401 atgacgccga tctaccggaa tataacgttg ccgttgaccg ttatgccgac tgggtggtgg
    11461 tgcaggagta tgcgccgcca aaaactattg atgctcacaa agcgcgtcag cgtctgttcg
    11521 atattatcgc tgcaaccatt tcggtactgg ggattgcgcc aaacaaactg gtgctgaaaa
    11581 cccgtgaacg ccagaagggc aaaaatcagt accagaaact gggcgagaag ggcgagtttc
    11641 ttgaagttac cgaatataac gctcacttgt tggtgaacct gacggattac ctcgataccg
    11701 gtctgtttct cgatcaccgt atcgcccgtc gtatgctcgg tcagatgagc aaaggcaaag
    11761 atttcctcaa cctgttctct tataccggca gcgccaccgt gcatgcggga ttaggcggtg
    11821 cacgcagcac caccaccgtg gatatgtcgc gtacttatct ggagtgggca gaacgcaacc
    11881 tgcgtttgaa tggcctgacc gggcgtgcgc atcgcctgat tcaggccgat tgcctggcgt
    11941 ggctgcgtga ggcaaatgaa cagttcgatc tgatctttat cgatccgcca accttctcta
    12001 actcaaaacg aatggaagat gcgtttgatg ttcagcgcga tcatctggcg ctgatgaaag
    12061 atttgaaacg tctgctgcgt gcaggtggga cgattatgtt ctcgaacaat aaacgtggct
    12121 tccgtatgga tctcgacggc ctggcaaaac tgggactgaa agcacaagaa attacgcaaa
    12181 aaacgctctc ccaggatttc gcccgtaacc gccagatcca caactgctgg ctgattaccg
    12241 cagcctaaag gaatagtaat gtcattaatc agtatgcatg gcgcatggct gtcgttcagc
    12301 gacgcgccgc ttctcgataa cgcagaactg catatcgaag ataacgaacg tgtttgtctg
    12361 gtgggccgca acggcgcggg caaatcgacg ttaatgaaaa tcctcaaccg tgaacaaggg
    12421 ctggatgacg gtcgcattat ttacgagcaa gatttgattg tagctcgtct gcaacaggat
    12481 ccgccgcgta acgttgaggg tagcgtttat gatttcgttg ccgaaggcat tgaagaacaa
    12541 gcggaatatc tgaaacgcta tcacgatatt tcgcgcctgg tgatgaacga cccgagcgag
    12601 aaaaatctca acgaactggc gaaggttcag gaacagctgg atcaccacaa cctgtggcag
    12661 ctggaaaacc gcatcaacga agtgctggcg caactggggt tagatcctaa cgttgcgctg
    12721 tcgtcgcttt ccggcggctg gttgcgtaaa gcggcattag gacgcgcgct ggtgagtaat
    12781 ccgcgcgtgc tgttgcttga tgaaccgaca aaccacctgg atattgaaac catcgactgg
    12841 ctggaagggt ttttgaaaac tttcaacggg acgattattt tcatctccca cgaccgttcg
    12901 tttatccgca atatggcgac gcgcattgtt gatctcgatc gcggcaagct ggtgacctat
    12961 ccagggaatt acgaccagta cctgctggaa aaagaagaag ccctgcgcgt ggaagaatta
    13021 caaaatgccg agttcgatcg caaactggcg caggaagagg tgtggatccg ccaggggatc
    13081 aaagcacgcc gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcgat gcgtcgcgag
    13141 cgtggtgaac gtcgcgaagt gatgggtacc gcaaagatgc aggtggaaga ggccagccgc
    13201 tccggtaaga tcgttttcga aatggaagac gtttgctacc aggttgacgg taagcaactg
    13261 gtgaaagatt tttcggccca ggttctacgt ggcgacaaaa ttgccctgat tggtccgaat
    13321 gggtgcggca aaaccacgct gctgaaactg atgctcggtc agcttcaagc ggacagcggg
    13381 cgtattcacg ttggcaccaa actggaagtg gcttatttcg atcagcaccg cgcggaactg
    13441 gatcccgata aaacggtgat ggataacctt gccgaaggta agcaagaggt gatggttaac
    13501 ggcaagccac gccacgtatt gggctatttg caggactttc tgttccatcc gaaacgggcg
    13561 atgacgccgg tacgtgcgct ttctggcggt gagcggaacc gcttgctgct ggcgcgtttg
    13621 ttcctcaaac caagcaactt attgattctt gacgaaccga ccaacgatct tgatgtcgaa
    13681 acgctggaac tgctggaaga actgatcgac agctatcagg gcacggtatt gctggttagc
    13741 cacgatcgtc agtttgtcga taacaccgtt acagaatgtt ggatcttcga aggcggcggt
    13801 aaaattggtc gttatgtcgg cggttatcat gatgcccgtg gtcagcaaga gcagtctgtg
    13861 gcgctcaaac agcctgcggt gaaaaaaacc gaagaagccg ccgcggcaaa agcagaaact
    13921 gtaaaacgca gcagtagcaa actaagctat aaattgcagc gcgaactgga gcagctaccg
    13981 caattgctcg aagatctgga ggcgaagctg gaagccctac agacgcaggt ggcggatgct
    14041 tccttcttca gtcagccaca tgagcagaca caaaaagtgc tcgccgatat ggctgctgca
    14101 gagcaggagc tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt
    14161 ggctgatcgc aaaatagtcg atttaaacag agacagctga caacggtaaa attgcctgat
    14221 gaactgggtt tatcaggcat gaaaaccaac actttgttag tcatctgtaa aatagcgcat
    14281 cattaaggag taccaatgtg cgaacatcat catgccgcga agcacatcct gtgctcgcag
    14341 tgtgacatgc tggtggcgtt accgcgcctt gagcatggtc agaaagcggc atgtccccgg
    14401 tgtggcacaa cgttaaccgt ggcgtgggat gcccctcggc agcgtcccac cgcctatgcg
    14461 ttggctgcac tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt
    14521 gcaggagtta ccagtgaaat tacattactg gaaattcccg gcgtgctttt ttctgaggac
    14581 tacgccagcc tcggcacctt tttcctgttg tttgtgcaac tggttcccgc gttttgtctg
    14641 ataaccattc tgttactggt gaatcgcgcg gaattaccgg tccgtttaaa agagcaactg
    14701 gcacgggtgc tttttcaact caaaacctgg ggaatggcgg agattttcct cgctggtgtg
    14761 ctggtcagtt tcgttaaact gatggcttac ggcagcattg gggtaggcag cagctttctc
    14821 ccctggtgtt tattttgtgt cctgcaactg cgcgcttttc agtgcgttga tcgtcgctgg
    14881 ttatgggacg acatcgcccc gatgccagaa ctgcgccagc cgctaaaacc aggcgtcacg
    14941 gggatacgtc aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc tgctgatgaa
    15001 cccgtgtgcc cgcgttgtag taccaaaggg tacgttcggc gtagaaacag cctgcagtgg
    15061 acactcgcgc tgcttgtaac gtccatcatg ctgtatcttc cggctaatat tttgcccatc
    15121 atggtggcgg atttattagg ctcgaagatg ccgtcgacga ttctcgctgg ggtcattctg
    15181 ttatggagcg aaggctctta tcccgtcgct gcggtgatct ttctggccag tattatggtg
    15241 ccaacgttaa agatgatcgc catcgcgtgg ctgtgttggg atgccaaagg gcatggcaag
    15301 cgcgacagtg aaagaatgca tttgatttat gaagttgttg agtttgtagg ccgctggtcg
    15361 atgattgacg ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg
    15421 agtatttatc cggcaatggg tgcattaatg tttgctttag tcgtcataat gacaatgttt
    15481 tctgctatga cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag
    15541 gagtcctgac agtatggaat ctaataatgg ggaagccaaa atccagaaag tgaagaactg
    15601 gtctcccgtg tggatatttc ctatcgtcac ggcgctcatt ggggcctggg ttctttttta
    15661 tcattacagc catcagggac cggaagtgac cctgatcacc gcgaatgcgg aaggaattga
    15721 aggtggcaaa accaccatta aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac
    15781 actggctgat gatttgacgc acgttgaaat caaagcgcgg ctgaattccg gtatggaaaa
    15841 attgctgcat aaagacaccg tcttttgggt ggtgaaaccg cagattggtc gcgaagggat
    15901 tagcggcctg ggaacgctgc tgtctggagt ttatatcgaa ctgcagccag gcgcgaaagg
    15961 cagcaaaatg gataaatacg atttgctgga ctcgccaccg ttggccccgc ctgatgcgaa
    16021 aggtatccgt gtgattctcg atagcaaaaa agccgggcag ctctcgccag gagatccggt
    16081 gctgttccgt ggctatcggg taggttcggt tgaaaccagc accttcgata cacaaaaacg
    16141 caatatcagc tatcaactgt tcatcaatgc accttatgac cgactggtga ccaacaatgt
    16201 tcgcttctgg aaagatagtg gcattgcggt tgatctgacg tcagcaggga tgcgtgtgga
    16261 gatgggctca ttgacaacgc tgctgagtgg cggtgtcagc tttgatgtgc cggaaggtct
    16321 ggatttaggg cagccagtgg caccgaaaac agctttcgtt ttgtatgatg atcagaagag
    16381 cattcaggat tcgttgtaca ccgatcacat tgattatctg atgttcttta aagattcggt
    16441 acgcggtctg caaccgggag ctccggtaga gttccggggt attcgcctgg gtaccgtaag
    16501 caaagtgcca ttctttgcgc cgaatatgcg tcagacattt aacgatgatt accgtattcc
    16561 ggtactgatt cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa atgcggatgt
    16621 tgttgagcac cttggcgaat tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa
    16681 cctggtcact ggtgcactgt atgttgatct cgatttctat ccaaatacgc ctgcaataac
    16741 cggtattcgt gaatttaatg gttatcagat tatcccgacc gttagcggcg gcctggcgca
    16801 aatccagcaa cgactgatgg aagcgttgga taagatcaac aaactgccat tgaatccgat
    16861 gattgaacag gcaaccagta cgctttctga aagtcagcgc acaatgaaaa acctgcaaac
    16921 gacgctggat agcatgaaca agatcctcgc tagccagtcg atgcagcagt tgccgacgga
    16981 tatgcagtca acgttgcgtg aattgaatcg cagcatgcag ggcttccagc ctggctccgc
    17041 agcctacaac aagatggtgg cggatatgca gcgccttgat caggtgttgc gagaactgca
    17101 accggtgctg aaaacgctca atgagaagag taacgcgctg gtatttgaag cgaaggacaa
    17161 aaaagatcca gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac gattgcagca
    17221 ctgtggctgg ccggatgcag ctccggcgaa attaataaaa actattacca gttacctgtg
    17281 gtgcagagcg gtacacaaag taccgccagc cagggcaatc gtctgttatg ggtagagcag
    17341 atcactgttc ctgactatct ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag
    17401 tatgtgattg ccaacaacaa cttgtgggcc agcccgttgg atcaacagtt gcgcaacacc
    17461 ctggttgcca acctgagtac gcaactgccc ggctgggtgg ttgcctccca gcctctggga
    17521 agcgcccagg acacgctcaa tgttaccgta acggagttta acggtcgcta tgatggcaag
    17581 gtcattgtca gtggtgagtg gctgttgaac caccagggac aactgatcaa acgtccgttc
    17641 cgtctggaag gagtgcaaac tcaggatggt tacgatgaga tggttaaagt gctggccggt
    17701 gtctggagtc aggaagccgc ttctattgca caagagataa agcgtctacc ttaattataa
    17761 agatttgtaa atataaccgt ctccggtatg ttgcctgagg cggttttttt gtctctaacg
    17821 tgcggaaaaa tttgttcctc ttcacatttt ttgtacaacc gacatgcccg tgtagctcac
    17881 aaatatgaca gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc ggtattgctt
    17941 aactgtgatt gcacatttag taatcactgt tttcttttcc accagaaacc agtatgaggg
    18001 aaacgaggca tgaagagaca aaaacgagat cgcctggaac gggcacatca acgtggttat
    18061 caggccggca tcgccggacg ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg
    18121 tcacaatggc tgggaggctg gcgagaagcc atggcggaca gggtagtaat ggcctgattc
    18181 tgtctcttta aaaagaaacc tccgcattgc ggaggtttcg ccttttgata ctctgtctga
    18241 ttataatcag aaggcagacg tatcctggaa cagaccgact ttcaggtcgc tggcggtata
    18301 gatcagacga ccatcaacca gcacttcgcc atccgccagg cccataatca gacgacggtt
    18361 aacaatgcgt ttaaagtgaa tacggtaggt cacttttttc gctgtcggca gtacctgacc
    18421 agtgaatttc acttcgccaa cgcccaacgc gcggccttta ccttcgccgc ccagccagcc
    18481 gaggtagaac cctaccagct gccacattgc gtccaggccc aggcatcccg gcataaccgg
    18541 atcgccaata aagtggcatc cgaagaacca cagatccgga ttgatatcca gttctgcttc
    18601 aacataccct ttgtcgaagt taccacccgt ttcggtcatt ttgaccacac ggtccatcat
    18661 cagcatgttc ggtgctggca attgcgggcc tttagcgcca aacagttcac cgcgaccaga
    18721 ggcaagaagg tcttcttttg tataggattc gcgtttatct accatgttct ctgtaagcct
    18781 tattttattg aagcacgcag gatagctaac acgtgtacgc tgaacaagtc cgatcagttc
    18841 ggaataaacc agttcagcca acgtaatggc catggaaaac ggtgacgtcc ttcctgttgc
    18901 gatgcttgcg cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc
    18961 cacaccagat ttaataataa cggcagtgcg tcagtcacat cgtctactgc ccagatggtg
    19021 aatttgcctt cttctaccgc tttcaccagt tcactgtgaa gacttaaatg gcgaacgtta
    19081 gctgtgggga taatgacacc ttgtttcccg gttaactcac gttgctggca aatagcaaag
    19141 aagccttcga ttttctcatt taaaccaccg accggctggg cgcgaccgaa ctgatcgact
    19201 gaacctgtga tagcgatact ctgattcacc ggcacatcgg cgagggcgct tatcagggcg
    19261 cagagttcag ccatcgaggc actatctcca tcaacttcac tgtatgactg ctcaaatgtc
    19321 agcgatgctg agaaggggat ctgttgctca agctgtagtt ccgacatcag gaacgcttgc
    19381 atgatcatca tccctttcgc atggatattg ccgccaagct ccgctttgcg ttcgatgtcg
    19441 gtgaattcac catcgccaat atgcacaacg cagctaatgc gagaaggttc gccaaaagcg
    19501 cgtggatgac ccggaaattc aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct
    19561 tcggtttcaa tcaggatttg ctcctgaagg atctcatcct gcatacgttc agcgaggaaa
    19621 ccttcgcgcc attcacgctg ctgcagcatt aagtttagct gctcgccgga gaaggtgtcg
    19681 ccatcacaca gggaggcgac ctctttacac tggcggagga tccactgcgg gctaagcgga
    19741 agtgtttctt gttcaccggt gtagcgtgct gcttcgcgga taagtaccgg ccaggcatcc
    19801 gctcccggtg caggtaagtg attatgtctg gcggtaaatg tcacccagcg acaccactgg
    19861 gttactgact ccgcatcgac aatctgcaga gtatcttcaa attcgctata aatagcctgc
    19921 tctgaaagct ctggctccat ctcctggaaa tcagccaatg attcgcgttc gcctaccaga
    19981 atgactttca gcttcaatgg catcgaaggc acagagacgg ggagagggcg cgactcatca
    20041 aacgcaaccc agtcaaaacg ctcgcggtta acgatatttt tcagccgcat ccacagcaga
    20101 ggttgcgcca gcagtgtacg caaagagatg atgagaatac cgccatttgc ctgatgcacc
    20161 agaccaggct gcagggtaat gtcgccatta aactggcgca ggcagccaaa gagttgctcc
    20221 gcttctaccc agtcggcagc gacaacttgc gttaaagtcg caaaattatc atctgcactc
    20281 actgcgtgac gtaagcggat ggagtggccg gaaacctcat agtgaccgcc caccagttgg
    20341 cctgcatcgc tttgtagcgt acgcgcggca ttggcaataa gattcagata ctcagactct
    20401 tccggggcct tcgccagcat aaaagaggag gatgctcgcg tatgcagcaa ctgctccagt
    20461 gcgaattgca gtcgcggttg agtatcactg aataaaggat cgttttcgtc aatcaaatgt
    20521 ggctgagcaa atatttcctg atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt
    20581 ttcgtaatgg tcaaagttga tgttttttag tctgttgtca aagccgcgat tataccgtaa
    20641 ccggcactac agcacacgta gaaagcaccg acaatactcc tggcatgggc gttaaagctc
    20701 acaggatgga gattcttttc ttcactggcc taaaaagctg atattctgta aagagttaca
    20761 cggtaacatt gagatcgcta tgaaatatca acaacttgaa aatcttgaaa gcggttggaa
    20821 atggaagtat ctggtgaaga agcatcgcga aggggagtta atcacccgtt acatagaagc
    20881 cagtgccgcc caggaagccg tcgatgtgtt gctctcgctc gaaaatgaac ccgtgctggt
    20941 aaatggctgg attgacaagc atatgaatcc ggaactggtc aatcgcatga agcaaaccat
    21001 tcgggcaaga cgaaagcgcc attttaatgc ggaacaccag catacgcgca aaaaatcgat
    21061 cgatctggaa tttattgtct ggcaacgtct ggctggtctt gcgcagcgtc gcggtaaaac
    21121 gctttctgaa acgattgttc agctgattga agatgcggaa aacaaagaga aatacgcgaa
    21181 taaaatgtct tctctgaagc aggatctgca ggcattgctg ggtaaggaat aactgacgaa
    21241 agtcagttca atttactaaa ggcaaaaaaa accccgcagc agcggggttt ttctaccaga
    21301 cgagaactta agcttgcggc tgagttacaa cgtctttgat acctttaact tcgatctcta
    21361 cgcgacgatc cggagccagg cagtcgatca gtgcagcacg ctgtttcacg ttgtcacagg
    21421 tgttgccagt aaccgggttg gattcgccca taccacgtgc ggagatcttg tctgccggga
    21481 tacctttgga gatcaggtaa tcaacaacag actgagcacg gcgctcggac agaccctggt
    21541 tgtaagcgtc agaaccgatg cggtcggtgt aacccagaac aactacggaa ccgtctttcg
    21601 gatccaggtt gctcagctgg ctgtacagct gatccagagc agcctgacct tccggtttca
    21661 gggttgcttt gttgaagttg aacagaacgt cagacttcag agtgaagtgc ttggtctgta
    21721 cttccggtgc cggagctgga gccggagcaa ctactggagc tgcttcgccc tgaccgaaac
    21781 ggtaggaaac acccaggctc agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt
    21841 caccgatgtt gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt
    21901 actcaacacc gccagcgaag accggagaaa cgccggtgtc gtggttttta ccataaacgt
    21961 tggatttagt gtctgcacgc cataccatac cacccagacg agtgtagatg tccaggtcgt
    22021 cagtgattgg gtaacccagt ttagcggtca gttgaacgcc ctgagctttg tatgcaccgt
    22081 tttcaacgct gcctttgtac ggcatacgac ctaaccagtc gtaacccatt tcaaagccaa
    22141 catacgggtt aacctggtaa ccaccaaaag caccagcgcc cagttggttt tcatgggtcg
    22201 ggccattgtt gttgatgaaa ccagtgtcat ggtactggga ccagcccagt ttagcaccag
    22261 tgtaccaggt gttatctttc ggagcggcct gcgctacggt agcgaaacca gccagtgcca
    22321 ctgcaatcgc gatagctgtc tttttcattt tttgcgcctc gttatcatcc aaaatacgcc
    22381 atgaatatct ccaacgagat aacacggtta aatccttcac cgggggatct gctcaatatt
    22441 aactctaccg atatcttcgg cttatgccga gcacccctgg cgatgtaaag tctacaacgt
    22501 agttgaaaac ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc ttgtataagg
    22561 tatgtttaat cttttttgtc agcgacaatt tacagaagaa aatcgcggaa accgcttcag
    22621 acaagcctcc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag
    22681 ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt ttagcccgga
    22741 aagttgtctc gtggcgtgag aggatgcgct taccggacgc ataataaacc ccatagcgtt
    22801 accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat catctgccaa
    22861 ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg actccacagt
    22921 gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc cagatgcctg
    22981 aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc gcgattgctg
    23041 accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct gatcttcgcg
    23101 atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac gcgcaatttt
    23161 acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt acataatcaa
    23221 tccagcccct gtgagttact gtatggatgt acagtacatc cagtgacaac aaagatcaac
    23281 cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac ataaattaat
    23341 cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtt
//
LOCUS       DAZFWP020000073        23374 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFWP020000073 DAZFWP020000000
VERSION     DAZFWP020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.073"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            312..1340
                     /locus_tag="ACHK0Z_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             312..1340
                     /locus_tag="ACHK0Z_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4502681.1"
                     /translation="MSELIISGSSGGFLRNIGKEYQEAAENFMRFMNDQGAYAPNTLR
                     DLRLVFHSWARWCHARQLAWFPISPEMAREYFLQLHDADLASTTIDKHYAMLNMLLSH
                     CGLPPLSDDKSVSLAMRRIRREAATEKGERTGQAIPLRWDDLKLLDVLLSRSERLVDL
                     RNRAFLFVAYNTLMRMSEISRIRVGDLDQTGDTVTLHISHTKTITTAAGLDKVLSRRT
                     TAVLNDWLDVSGLREHPDAVLFPPIHRSNKARITTTPLTAPAMEKIFSDAWVLLNKRD
                     ATPNKGRYRTWTGHSARVGAAIDMAEKQVSMVEIMQEGTWKKPETLMRYLRRGGVSVG
                     ANSRLMDS"
     gene            1450..1758
                     /locus_tag="ACHK0Z_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             1450..1758
                     /locus_tag="ACHK0Z_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502682.1"
                     /translation="MDGYTVTFAAGVKNSHAVVVFGIMTGLEAVPVFTLEKNWRNLEE
                     AESYVRKVTIIAAEKLLAHYQENYRTMVEKINLAFTRPTTGFIAPDTNAGRYPARGRR
                     "
     gene            1755..2231
                     /locus_tag="ACHK0Z_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             1755..2231
                     /locus_tag="ACHK0Z_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502683.1"
                     /translation="MKTYYPSLNCYPSSYPELSGKLLALLAKAPTEWFKPVVINPHFL
                     DYGSVRTRNELKNLLAHGFIRHRAGKGYQLAIAPEEAVRLFAYRDSQTKARVIAQILH
                     NGSTYARQFGGDTSQFLRAVRSLEGQGVIESSCLPVPTVPHIKRRVYTFTQRAKKQ"
     gene            2228..2722
                     /gene="dut"
                     /locus_tag="ACHK0Z_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             2228..2722
                     /gene="dut"
                     /locus_tag="ACHK0Z_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4502684.1"
                     /translation="MIYVKVKRLHPAAKLPAYATSGSAAMDFEAVEIKPCVDSNGAIS
                     SSWWVYTGLAMEIPPGWCLKLYPRSGLGCKKHTRLANCVGIIDSDYRGEIMAKLITDP
                     GGEGVCLKPGMAVMQGIFERVEQVSLVEVEELNETERGAGGFGSTSEGHFEIKRSPLQ
                     YEKL"
     gene            2737..3438
                     /locus_tag="ACHK0Z_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             2737..3438
                     /locus_tag="ACHK0Z_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGU4502685.1"
                     /translation="MNNMATKELFKFLPKYKAHKNVSASKIKNVVIFGVTDAPLWGCI
                     ELVEPKGATVCVDAMFLQKHRPKTVGYLIVYENGYLFYAPEKPFEERYGLVNDASKNG
                     TTVIITDDNGVMINLTGSVTISAGGITTQQEEIDLEAADFSDALMWLKDGKKVARRGW
                     NGENQFCWLVPDKQGASRLTPIKHAANGELEINFDSPLAPYGAHFVLKNAQGVVVPWV
                     PSVGDLLACDWFVVE"
     gene            3445..4221
                     /locus_tag="ACHK0Z_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             3445..4221
                     /locus_tag="ACHK0Z_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502686.1"
                     /translation="MESTKTIQYRLRNGLLVAVNADMSLPFDTIERTIMTYLGFNEEL
                     NEEHGVAIWSDADSGVRRYITARGKDYSLEELFTLAQSFECVALDLFNDPAIAQRLIR
                     ELGLSVTPIIFKNGSLTGTWRVERISNYLPYNRQLNGVISGVNQPVACENVNLVVAVL
                     ATACRVIGLAKQAFIHFPNGAEGSAEIIACDFEFTWMLREYLDQTVFRAEELDMYITS
                     TIPDDVRAEAIAIARAKCRAAIAERAKEEQSNDTAAEEVK"
     gene            complement(4254..4451)
                     /locus_tag="ACHK0Z_RS13435"
                     /old_locus_tag="pgaptmp_002687"
     CDS             complement(4254..4451)
                     /locus_tag="ACHK0Z_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HGU4502687.1"
                     /translation="MARKYNKLSREALKMLLDGVSRSEVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMLGSRQSASSI"
     gene            complement(4456..4731)
                     /locus_tag="ACHK0Z_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             complement(4456..4731)
                     /locus_tag="ACHK0Z_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502688.1"
                     /translation="MSKINLRTESAVFKNPTFNVPKQTLDELVPIDSRFVIPAELVEN
                     CKEKQKSLKEISPSEILSRSDVSKEEVLRAISASTATTLRRLYGSAE"
     gene            complement(4751..5131)
                     /locus_tag="ACHK0Z_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             complement(4751..5131)
                     /locus_tag="ACHK0Z_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4502689.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVV
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELVDLTV
                     GAATGEIAVSSVAEKLRELYGSKT"
     gene            complement(5131..5352)
                     /locus_tag="ACHK0Z_RS13450"
                     /old_locus_tag="pgaptmp_002690"
     CDS             complement(5131..5352)
                     /locus_tag="ACHK0Z_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4502690.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(5425..5814)
                     /locus_tag="ACHK0Z_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             complement(5425..5814)
                     /locus_tag="ACHK0Z_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4502691.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGCT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            complement(6084..6368)
                     /locus_tag="ACHK0Z_RS13460"
                     /old_locus_tag="pgaptmp_002692"
     CDS             complement(6084..6368)
                     /locus_tag="ACHK0Z_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin PHD"
                     /protein_id="HGU4502692.1"
                     /translation="MPTKAELQARVEILEKENASLKGMLARAERELSGKLLPEELPPA
                     DIPDRVSRWMKYFRAPWEAFWCYDHRRWCDELDSSFPYFAEGNSCPECRS"
     gene            complement(6352..7101)
                     /locus_tag="ACHK0Z_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             complement(6352..7101)
                     /locus_tag="ACHK0Z_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502693.1"
                     /translation="MTRITKERISDIISRIEMYGHGAGYTADEVLELAKIALAPCKDG
                     KAMTLIDLLVKELSKGRGWPDGKDFCHLSITMPGSSRATVLFGTRHKNYQSSKNYSID
                     GIICDISDLDRSAFTSVVTREQYEAARIASQKVKSESKAYKLDFEQWLEQQRGEIDVD
                     CGCVSAETFMHWLRVAYEAGNHPVLPDSSQQAPRKSVKTTLERGYLEAALKIKPGHTL
                     GVIDAMLVHEMAKALLPLVADKHEAGHANES"
     gene            complement(7098..7307)
                     /locus_tag="ACHK0Z_RS13470"
                     /old_locus_tag="pgaptmp_002694"
     CDS             complement(7098..7307)
                     /locus_tag="ACHK0Z_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502694.1"
                     /translation="MATFSKFAVIIILLIPLTVTAGEQQHCTKENEHPFIVIQCDDGT
                     VTVVNVRNDRVAVCRKGEPCKEIKL"
     gene            complement(7332..7625)
                     /locus_tag="ACHK0Z_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             complement(7332..7625)
                     /locus_tag="ACHK0Z_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502695.1"
                     /translation="MWRGNNHGGSQMILTEYTFDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNLGNFKPTISLDDFPCGLSEREAMLKLAEWLQRLSIAIEDNWIQP"
     gene            complement(7649..7930)
                     /locus_tag="ACHK0Z_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             complement(7649..7930)
                     /locus_tag="ACHK0Z_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4502696.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKREDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVKINS"
     gene            complement(7923..8495)
                     /locus_tag="ACHK0Z_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             complement(7923..8495)
                     /locus_tag="ACHK0Z_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4502697.1"
                     /translation="MTTITKERLLTIKRWRETYGPGSNVVLPAEEAEELVRIALASLE
                     AEPASQTYNLPELIEGMEVSIDVSTCDADLGNRYFGTVTEALELDTAKNGYILLVQDA
                     EPNFDVNGNSPGTPDSWISCSDQMPEKGQNVLISVNFDSSLVEPLICSARYTGSTFRR
                     GDATIKPGNGIEQATHWMPLPEPPQEVNRG"
     gene            complement(8829..9007)
                     /locus_tag="ACHK0Z_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /pseudo
     CDS             complement(8829..9007)
                     /locus_tag="ACHK0Z_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9009..9197)
                     /locus_tag="ACHK0Z_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             complement(9009..9197)
                     /locus_tag="ACHK0Z_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502698.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            complement(9370..10179)
                     /locus_tag="ACHK0Z_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             complement(9370..10179)
                     /locus_tag="ACHK0Z_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4502699.1"
                     /translation="MIKINYQELREAAEQATQDEWVAYILPGHNGIYPARTSEGRHCG
                     YFIDWPGIDGQRNAGANARYIASIPPKVALALLAEIKRLEDTNIDAMCRIAELEKQCT
                     EWERKALSNFEECAAMAERIEELQTNSAPDSFGIIGENIRTQDNRITSDPMFCVYQKR
                     EIVVDADYDYDRIVWVDEDGNEANKRQSRRLELLHENFREPPEKWRRVAVKDIDEFVT
                     CCFTEQGCKDYLAANGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGGE"
     gene            complement(10166..10465)
                     /locus_tag="ACHK0Z_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             complement(10166..10465)
                     /locus_tag="ACHK0Z_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502700.1"
                     /translation="MSQGNGDGAAKCRISTCLINGKKIEPCAALAQSLEQDAEYTTRK
                     GLLKYKIYNHELIHSQDLIMLRSGEFSKSPIRISFCPFCGESLKTWEAEATSDQN"
     gene            complement(10479..11432)
                     /locus_tag="ACHK0Z_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             complement(10479..11432)
                     /locus_tag="ACHK0Z_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4502701.1"
                     /translation="MAVFPVDKPTSNYIGAGRRFLISPLPREQAENTPDGVVDLNYSL
                     VANQSLAPFFQSERVFNALGGEDSLVHWVSTNIHDCQAHDKRDCSHQLTTHFYNGSAV
                     RLCWKHDAEYMMKGYGKLEDQLSRNRANWIMNWAASELKLPPDRDLSMVELTFWAIRR
                     NLKDELPDEAGRIAFCQPKTEIPTGTLKESDITWEHSTRELVDISAEQIVNLSVDEDS
                     GLLYMRRPKTLLGKSPAYLRFVVSRPCIGCGGKVNHPFMYRARSLNEHDRWAVPLCDE
                     CARSAENDVRAWEKTHGIRLYVAANQLFDFAIERGVITFNN"
     gene            complement(11573..12265)
                     /locus_tag="ACHK0Z_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             complement(11573..12265)
                     /locus_tag="ACHK0Z_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU4502702.1"
                     /translation="MIQTMHEVNLYSRIDGSGYRNIWVVGDLHGCYTRLMSELHRVDF
                     DPAQDLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMLDALSPDGNVNNWLM
                     NGGQWFFMLGTDQEILAKALVELVKRLPYIIELNTGHETIVIAHADYPGGEYQFGKDV
                     SLFDVVWSRSRVGDSIDGIGGEITGADRFIFGHTPVRRPKAYWNQHYIDTGAVFCGNL
                     TLMQVKGGKLKV"
     gene            complement(12262..12573)
                     /locus_tag="ACHK0Z_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             complement(12262..12573)
                     /locus_tag="ACHK0Z_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502703.1"
                     /translation="MSIIKEMPVERDEYGCWTHPEYEKFCAGREYISTEEFDAWMKEN
                     NLQWTIRSMDEDDFDLDAAGPDIAAWEPERPEGEGWFVGSIHDTEDGPVCIWLREKVA
                     A"
     gene            complement(12570..12794)
                     /locus_tag="ACHK0Z_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             complement(12570..12794)
                     /locus_tag="ACHK0Z_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502704.1"
                     /translation="MTEQTMTNRELVDAAIELAGDFYSMMGYEHRPGFKYWESPHPQE
                     QQVFEMACRAFEVIRGSDVMEAVADLEDEE"
     gene            complement(12791..13066)
                     /locus_tag="ACHK0Z_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             complement(12791..13066)
                     /locus_tag="ACHK0Z_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU4502705.1"
                     /translation="MNIDTTITIDTALNTGLALFGWLYIMSRTWRWLGSIFLKEWKKR
                     RKQELRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQEKTK"
     gene            complement(13063..13311)
                     /locus_tag="ACHK0Z_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             complement(13063..13311)
                     /locus_tag="ACHK0Z_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502706.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMVLKAEKQEPPQ"
     gene            complement(13308..14060)
                     /locus_tag="ACHK0Z_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             complement(13308..14060)
                     /locus_tag="ACHK0Z_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502707.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGSIGSTPSVEV
                     QSIHAGFDWDSGKVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSIELDTAKQRIAELEGNRAALAAENAGLNKFIAQSCYVFDGEQDEISDAYICATDGG
                     IPQTPATDAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTE
                     FMANAPEGDFDRSFSDGVLEDIAAQLGKGGKQ"
     gene            complement(14069..14479)
                     /locus_tag="ACHK0Z_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             complement(14069..14479)
                     /locus_tag="ACHK0Z_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502708.1"
                     /translation="MKRMIFVAALLTITQQAQASAVIVASTAATTAAVAAANSANIAN
                     QQSQRAANASASVHPIAIKTGKKNIGFITCGKRSDEAVGSLGCTVYGDSESREIPWKT
                     WPGYVLGSKLPASYEVNAVSFDHYNGVATVYFAY"
     gene            complement(14476..14964)
                     /locus_tag="ACHK0Z_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             complement(14476..14964)
                     /locus_tag="ACHK0Z_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502709.1"
                     /translation="MKSEGLTPAQLAERNAEYVTEISRLEKACAALAAENAGLKHAMA
                     VTLEHVSVTDAGQAGVAAMIINDALHHSETPATDAFLAEIRAAARNEGINYTASRLAA
                     AFNHGFINKSLREVFDVTRMILSAKEELANEAHPIDGLSGEYAEKSLEEWAEQIRKGA
                     GK"
     gene            complement(14954..15238)
                     /locus_tag="ACHK0Z_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             complement(14954..15238)
                     /locus_tag="ACHK0Z_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4502710.1"
                     /translation="MILRQCAGTMTVESIGKLIGRTGDAVRTKARELGISMILKGDFH
                     PSAKYRQSDIELTRQLHQCGVPRREIAEKLEMPLGMINQYVYFERRVYEV"
     gene            complement(15268..15891)
                     /locus_tag="ACHK0Z_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             complement(15268..15891)
                     /locus_tag="ACHK0Z_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4502711.1"
                     /translation="MVKRVLKIYIAGPMTGYPDYNRAAFNAKASELMAEGHIVLNPAV
                     LPGGLCQSEYMDICLAMVRSADAIYLLNQWEESVGARAEHALAEKLGLTVIYESPTNI
                     ECQVATHIYRELVNALRDVAIECHGTDQLRARLSQTLSSYLSLAEGYNLRQRRMVRLI
                     TRLSQSLANAEPTNPLPNDAMNYLKSCGVVSEDAVRFVEFMSQRMSA"
     gene            16164..16760
                     /locus_tag="ACHK0Z_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             16164..16760
                     /locus_tag="ACHK0Z_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family recombination enhancement nuclease"
                     /protein_id="HGU4502712.1"
                     /translation="MQKQSYWEKQSYWEKQRQKAMQKLADPAWREEQRAKRLQQAQRQ
                     QQRAREKAASPEYRQKKIEKAKQYDQRRKEKAVSAPSKKTRTSRGLKGRSLTADERRI
                     QTAIGTLPCIACHIHGQHSPVVSLHHIFGRTAENAHKYVLPLCKWHHQYAAPAEVREQ
                     YPWLVPVHADGKIGGKADFMRHNADEMALYQMAIELIN"
     gene            16900..17058
                     /locus_tag="ACHK0Z_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             16900..17058
                     /locus_tag="ACHK0Z_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502713.1"
                     /translation="MAEKPPLTHGVPAFIAPPPDRGTVDGGSESKESLHYEQLQSFPI
                     TRRPPLTR"
     gene            17092..17259
                     /locus_tag="ACHK0Z_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             17092..17259
                     /locus_tag="ACHK0Z_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502714.1"
                     /translation="MTVRYLNFQIKNITGGCYDWFVTLGKEVITGKLDEVKAKAMAYA
                     CKQARKKSAKA"
     gene            17434..17553
                     /locus_tag="ACHK0Z_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             17434..17553
                     /locus_tag="ACHK0Z_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGU4502715.1"
                     /translation="MVVLAGLPKGRPVPFCAGSLNPASATAIEIETSRGSSLN"
     gene            17572..17787
                     /locus_tag="ACHK0Z_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             17572..17787
                     /locus_tag="ACHK0Z_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4502716.1"
                     /translation="MATIPTPTHSEFIWRFYSCQKHLYICVMAATESEARSYLPEEPC
                     IFAARFTLDAMEILNYWNLPMNCVEVH"
     gene            17787..18497
                     /locus_tag="ACHK0Z_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /pseudo
     CDS             17787..18497
                     /locus_tag="ACHK0Z_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            18788..19072
                     /locus_tag="ACHK0Z_RS13595"
                     /old_locus_tag="pgaptmp_002719"
     CDS             18788..19072
                     /locus_tag="ACHK0Z_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4502717.1"
                     /translation="MKKIDFTYSAATIQRRFSLIREVELSKNWYQILLDEEFSLMVIA
                     EKLAMPNDRHKVIASLDLVTNRYWESEELLEVGLIREMIEQAVPLHLQQP"
     gene            19209..19568
                     /locus_tag="ACHK0Z_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             19209..19568
                     /locus_tag="ACHK0Z_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502718.1"
                     /translation="MIIVATKIKLENPQTGESITGFYGFSWTTFLFGAFPALFRKDFI
                     TFIGVFVVMLILAFLTAGIGPWIASFIWAFMYNKYFTVNKIKQGFIFAGSHTENELAA
                     SKLGLSLNQNNCKTITE"
     gene            complement(19609..20430)
                     /locus_tag="ACHK0Z_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             complement(19609..20430)
                     /locus_tag="ACHK0Z_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502719.1"
                     /translation="MFFIKNGKRCSSTILATVVALSVPTFAFAYDFSKIDCNAPKTRQ
                     MLIDDYNELLKDDQEAISVIDAYNQINEIREKNKLQCLGTYEFSDGSALRVRYKLYLN
                     SLGTPIYEFAPVEELTQDDVSASIQNIPATTASKAGGNEETYSCKVAVTYNGKRSNYM
                     GDTGVWDRVITDYGTYFSWDLPRGNRGNSTGQDAFSGMDELKPALENKLVRKEVEKDG
                     SVVDEFRTDVSYGEKQPVHKFVYARRIKPTGMREYYVTDLTDKRAFMFLNCQRDS"
     gene            20777..21430
                     /locus_tag="ACHK0Z_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             20777..21430
                     /locus_tag="ACHK0Z_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HGU4502720.1"
                     /translation="MEKIAIFSLTTSKPQMLTAILKDGALVINEVKPLPASALEQKQK
                     IPPAIAALRKSKFKVLVDEITPTISAGTGASQVTLKTRHADGRAAIIVGMERYRELKL
                     QKLLSLPQNNKGAFEIPDSIVDTEYNGNGEEVYRVNWQDIRPEHILMILCCYATVYHN
                     VASADYVEQMTGTVEKEQKTGILASFLSIIGHEKVKAGTSQPKSLTGKEVDEDTVIL"
     gene            21759..22088
                     /locus_tag="ACHK0Z_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             21759..22088
                     /locus_tag="ACHK0Z_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGU4502721.1"
                     /translation="MGYSRLDDRYIEDDIFRALFHQEMIKRVSEYHSDNFQYTIKIDE
                     VYRSDLAAYRAYGNADLRWVFRVLVGHESEMEEMPAGTTLTLPDVAWLRNKIRDYASA
                     EPEIENA"
     gene            22081..23274
                     /locus_tag="ACHK0Z_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             22081..23274
                     /locus_tag="ACHK0Z_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4502722.1"
                     /translation="MPDFLKNQDGRYITDGLSSKDFTRLFDLIRKEQTRKRRQAHRTL
                     TPGRLRNKSAEDILKLGKKKGGTFFTRDDLKGFEKLRSKTREKYDSKTAGITYAQLVA
                     SSQAIDIKRANNAVDDGSGIKRATPVSLRHNVINIRVEASDISVHQHHIVRIRFEEWD
                     QMVDDIAEDDKSALKITKSLCAGRVSFDCDCGRHQYWYRYIATAGNFALAPPKEYAYP
                     KVRNPKLQGVACKHVIHAMTRLQSASWQMSIARALQKAATQIAFGDDRRRTTKHFSKE
                     DEKEFNRNRSSKTNVDAAKREWKLYQKRQAALSEKLAKDNGKNDKLRDQLTRARKLSD
                     AQKKRAAAKEAALQREKQKNKELQQRLADQFALKKQAFIDALVMAGTPPAQAEKMFME
                     YVKKA"
BASE COUNT         6256 a         5857 c         5183 g         6078 t
ORIGIN      
        1 gtgatttttt gtcatcaatt ttagaaaacc aactgtataa accacacttc gcaaattaaa
       61 gaccatgaag gcaacgtgtt actggtcaaa atcaatctat gatggattaa aaaaaatcgc
      121 cattttgtaa ctttaaataa ttggcattat ttaaagttag gctataatca tgtgtagatg
      181 aacagtgttg acatcactga aaatacgtgc ctcgaatcaa aggcttgtat tttgaaaatt
      241 aacttatagc taccctgtca tttttatatt tttcgcgtag cgtcatctgt atatagaggt
      301 gaaatagttt catgagtgaa ttaattatct ctggcagttc cggtggtttt ctgcgcaaca
      361 ttggcaaaga gtaccaggaa gccgcagaaa actttatgcg gttcatgaat gaccaggggg
      421 cttatgcgcc gaatactctg cgcgacctcc ggttggtgtt tcattcctgg gcgcgctggt
      481 gtcacgctcg ccagcttgcc tggtttccga tctcaccaga aatggcccgc gagtattttc
      541 ttcagctgca tgatgccgat ctggcttcga ccaccattga taagcactac gccatgctga
      601 acatgctgct ttcgcattgt ggccttccac cactttcgga tgataaaagt gtttctctgg
      661 ctatgcggcg catccggcgc gaagcggcaa ctgaaaaagg cgaacgaaca ggccaggcta
      721 taccgctgcg gtgggacgat ctgaaactgc tcgacgtcct gttgtccagg tcagaacggc
      781 tggtggacct gcgcaaccgg gcttttctct ttgttgcata caatacgctg atgcgtatgt
      841 cggaaatctc gcgtattcgt gtaggagatc tggaccaaac aggtgacact gtcacgctac
      901 atatttcaca cacgaagaca ataacgaccg ccgccgggct tgataaggtg ctttcccgtc
      961 gcactaccgc tgtgctgaat gactggctgg atgtttccgg gcttcgcgaa catcctgacg
     1021 cagtgctgtt cccgccgata caccgtagca ataaggccag gatcacgaca acgcccctta
     1081 ctgcacctgc aatggagaaa atattcagcg acgcctgggt gttgctgaat aaaagagatg
     1141 ctacgccaaa caaaggaaga taccggacgt ggaccgggca tagtgcacgt gtcggggccg
     1201 ctattgatat ggctgaaaag caggtgtcta tggtggagat catgcaggaa ggaacctgga
     1261 agaaaccgga aacacttatg cggtatcttc gccgtggcgg cgtgtctgtg ggggctaata
     1321 gccgcctgat ggattcgtaa attaaatttc taacacatct ttttagatta acgcctttgc
     1381 tgtaaagtac cctcgcctat ttaggagggt gctttgcaga gtcaaatcac ggaatcaaga
     1441 cgcttcaagg tggatggtta caccgtaacc ttcgccgctg gtgttaagaa cagtcatgct
     1501 gtcgttgtgt tcggcatcat gaccgggctt gaggccgtcc ctgtgtttac cctcgaaaaa
     1561 aactggcgaa atctggagga agcagaaagc tacgtccgga aagtaaccat cattgccgcc
     1621 gaaaaactcc tggcccacta tcaggaaaat tatcgcacta tggtcgagaa gattaatctc
     1681 gctttcactc gccccactac cggctttatt gcaccggata ctaatgcagg tcgatatcct
     1741 gcaagggggc gtcggtgaaa acttattacc catctctgaa ttgttacccg tcttcctatc
     1801 cagaattatc cgggaagctg ttagctctat tagctaaagc acctacggaa tggtttaagc
     1861 cagtcgttat caatccacac ttcctggatt acggatctgt tcgcactcgt aacgaactca
     1921 aaaacctact ggcgcatgga ttcattcgac accgtgccgg gaaaggctat cagttagcga
     1981 ttgcgccaga agaggccgtg cggctattcg catatcgtga cagccagaca aaagctcggg
     2041 taatagctca aatacttcat aacggcagca cctatgcccg ccagtttggc ggcgacacgt
     2101 cgcagttctt gcgggcggtg cgatcgctgg aggggcaagg ggttattgaa agttcctgtc
     2161 ttcctgttcc taccgttcca catatcaaac gtcgggttta caccttcacg cagagagcta
     2221 aaaaacaatg atttacgtaa aagtgaaacg cctacatccg gcagcaaaac ttccagctta
     2281 tgccacctcc ggatcggcgg cgatggattt tgaggctgtt gaaataaaac catgcgtcga
     2341 tagcaatggt gcaatttcct caagctggtg ggtgtatacc ggactggcta tggagatccc
     2401 gcccggctgg tgtctgaaac tctatccgcg atccggattg ggctgcaaaa aacatactcg
     2461 cctggctaac tgcgtaggaa ttatcgactc cgattatcgc ggtgaaatca tggcaaaact
     2521 gattactgat cccggcgggg aaggcgtttg tctcaagcct ggaatggctg tcatgcaagg
     2581 catttttgag cgtgttgaac aagtctcact agtcgaggtt gaggaactca acgaaacgga
     2641 acgtggtgct ggtggctttg gtagcacatc ggaaggccac tttgaaatta agcgttcacc
     2701 gcttcagtac gaaaagttat aagcagagaa aagactatga ataacatggc aacgaaagag
     2761 ctttttaagt tccttcccaa atacaaagct cacaaaaacg ttagtgcatc gaaaatcaaa
     2821 aatgttgtta ttttcggagt aactgatgcc ccactttggg gctgcatcga actcgttgaa
     2881 ccaaaagggg caacagtttg tgttgacgca atgtttttac aaaaacaccg accaaaaact
     2941 gtcgggtatc tgatcgttta tgaaaacggt tatctgttct atgccccgga gaaaccattt
     3001 gaggaacgct atggcctggt taacgacgcc tccaaaaatg gaaccacggt catcattacg
     3061 gatgacaacg gcgtgatgat taatcttacc gggagcgtaa ctatttctgc tggtggcatc
     3121 accacccagc aagaagaaat cgaccttgaa gcagccgact tttctgacgc gctgatgtgg
     3181 ctgaaggatg gcaagaaagt tgctcgacgc gggtggaacg gcgaaaacca attctgctgg
     3241 ctggttcctg acaaacaggg tgcttcaaga ctaaccccta taaaacatgc cgcaaacgga
     3301 gagcttgaaa tcaatttcga ctctcctctt gctccttatg gtgcgcattt cgttttgaaa
     3361 aatgcacaag gtgtagttgt tccgtgggtt ccttctgtag gcgacttact ggcatgtgac
     3421 tggtttgtag tggagtgatt tgacatggaa agcaccaaaa caattcaata ccgcttgcgt
     3481 aatggcctgc tggtggctgt taacgccgac atgtcactgc cgtttgacac gattgaacgc
     3541 actatcatga cgtatctcgg ctttaatgaa gagctgaatg aagagcatgg cgtagctata
     3601 tggagtgatg cagacagcgg cgttcgtcgt tacatcaccg ccaggggcaa agactactct
     3661 ctggaagaac tgtttaccct tgcgcagtct ttcgaatgtg tagcattaga cctgtttaat
     3721 gatcctgcta tagcgcaacg acttatacgc gagcttgggc tatccgttac accaattatc
     3781 tttaaaaatg gcagcctgac tggcacatgg cgcgtagaac gcatctcaaa ctaccttcca
     3841 tataaccgac agttaaatgg agtaatctcc ggcgttaatc agcccgtagc gtgtgaaaat
     3901 gtaaacctgg ttgtcgctgt tttggcaacc gcatgtcgtg ttatcggttt ggctaaacag
     3961 gcatttattc atttcccaaa tggtgcagaa ggtagcgcag agattatcgc gtgtgatttc
     4021 gaatttacct ggatgctacg cgaatatctg gaccaaacag tattccgtgc cgaagaactg
     4081 gatatgtata tcacgtcaac gattccagac gatgtgcgag ctgaagcaat cgctatagcc
     4141 agggcgaaat gccgtgcggc gattgcagaa cgggccaaag aagaacagtc caatgataca
     4201 gcggcagaag aggtgaaata acaaaaagcc cgcttggtat ggcgggcttc ttttcaaata
     4261 ctggaagcac tttgtctgct accaagcatt ctctgtttaa gcacaaccat ttcctgacgg
     4321 cataacacag caatagcggt cctggctcca atttgcttac caaccaagta ttgctttacc
     4381 tcgctgcgac tcacgccatc aagaagcatc tttaacgctt cacgggacaa cttgttgtat
     4441 ttacgtgcca ttaatctact ccgcagaacc atacaatcta cgtagcgtgg tagcagtaga
     4501 tgcagatatc gcccttaaaa cctcctcctt acttacatca ctgcgggaga ggatttcaga
     4561 aggcgatatt tcctttaacg acttttgctt ctctttgcag ttctcgacca gttccgcagg
     4621 aataacaaac ctgctatcaa tcggcacaag ttcatcaagc gtttgtttgg gtacgttaaa
     4681 cgtaggattc ttgaacaccg cagattcagt tcttaagtta attttcgaca ttgtttcgta
     4741 actccactac tcaagtcttg gaaccatata gttcacgtag cttttctgcg actgaagata
     4801 cggcgatttc accagttgca gcccctacgg taaggtctac cagttcaggg gagtcaaata
     4861 cctgcacccc gttacggcgt agaaataaca gcgcactgtt tagcgcggta cgcttattgg
     4921 catcattgaa tatatgccct ctcgcagtag ccaccaggta ggttgcggag acttcgaaaa
     4981 ggtcggtaat ctcttcgtac acaactctgg cctggactct cccgataatg gcctctgccc
     5041 tgcccggatc tgacatgcca ggcaggccgc cgtagcggtt tatattcgca tcatgaagcg
     5101 caataagttc ttccggtgat atatgcctca ttatcggtta accagttcct tgttggtgga
     5161 gtccagggtg tcaaacaggg atgcaaattc agcatccagc gccgcttttt tgtaggcttc
     5221 gaaagtagcc ttgctgacaa ttactgctgg ctcacggcct ctgcgggtga tttcaacctc
     5281 ttccccggct tcaacattgt tgagcacttc agaaaggttg ccgcgcgcgg tacggaagtt
     5341 aatggattgc ataaacacct cgtgtactcg ttatgtgtac acaattataa acctcacagg
     5401 cataaagcac cagccctttg cagcttaaat aaccggacaa tcatcaaact ccccacttcg
     5461 ggcatcattg atgacatgag tgatcacacc aaaaacagca ttactgcccg tgcatccatc
     5521 gtcatctact ggtaacgcct ctttcttccc ggtgcttaaa tcctccaggt gctggcgcgg
     5581 atacttcctg tatctcttta tgcgatattc accctccatc gcgcagacaa gtagcgaacc
     5641 atcaaccgga gtaagtgagg aatcgaccac cagcaaagca ccctgcaata ttccctcacg
     5701 gtgatggcta tcagctgccc gcatgaagta ggttgctgat ggatgcctga ttagttgctg
     5761 atcaagagaa attcggcttt caacataatc cgccgcagga gaagggaagc ccatagcgtt
     5821 ttcacctcaa taatactgtt catttataca gtatacatta aagggacacc tttggtgcaa
     5881 acgcgttacg tacatcaacc accgctgatg attttgtgct ctttgctact attcatcacc
     5941 aacggatcag cgtaacctcg ttgccaatca gttaataagg aattagctat gcctaatcgc
     6001 attcctctcg atcctgtatt gcccaaaaat tttgactgca ctcctaacga gaaacatttt
     6061 aaaataaagc tcgccggtca tcattaactc ctacattcag ggcaagagtt cccttccgca
     6121 aaatagggga aactgctatc aagctcatca caccatctgc gatggtcgta gcaccaaaac
     6181 gcctcccacg gtgcacggaa atacttcatc cacctggaca ctcgatcagg tatatctgct
     6241 ggtggcagct cttctggcaa taatttgcct gataattccc tttccgcccg cgccagcatt
     6301 ccttttagac tcgcattttc tttttcaaga atctctacgc gagcctgtaa ctcagctttc
     6361 gttggcatgg cccgcctcat gtttatcagc caccagcggc aataaagcct tagccatttc
     6421 atgaaccaac atggcatcaa tgacgcctag cgtatggccc ggcttaatct ttaatgcggc
     6481 ctcaagatag cctctttcca gagtggtttt tacgcttttc ctgggcgctt gttgggaact
     6541 atccggaaga accggatggt tcccagcctc ataagctacc cgcaaccagt gcatgaatgt
     6601 ttccgcagac acacaaccgc agtccacatc gatttccccg cgttgctgct ccagccattg
     6661 ctcaaaatct aacttgtaag ccttactttc agacttgact ttctgagacg ctatgcgagc
     6721 tgcttcatac tgttcacgag tgacaaccga tgtaaacgca ctgcgatcaa ggtcactgat
     6781 atcgcaaata atgccatcaa tggaataatt ctttgatgat tggtaatttt tatggcgcgt
     6841 accaaaaaga accgtagcgc gactactacc tggcatcgta atggatagat ggcaaaaatc
     6901 ttttccatca ggccatccac gccccttaga taattccttc actaacaagt caatcagtgt
     6961 catagcttta ccatccttgc aaggcgctaa agctattttg gcaagttcca atacttcatc
     7021 tgctgtgtat ccagcaccgt gaccatacat ttcgatacgg gaaataatat ctgaaatacg
     7081 ctctttagtg attcttgtca tagttttatt tccttgcatg gttcgccttt acggcaaaca
     7141 gctacacggt catttcttac attaaccaca gtcaccgttc cgtcatcgca ttgaataacg
     7201 atgaaagggt gttcattctc ttttgtgcaa tgttgctgtt cccctgctgt aacagtgagg
     7261 ggaattaata aaataataat tactgcgaat ttactaaatg ttgccatagc aatctttctt
     7321 tctgtttttc attatggttg aatccagtta tcttcaatag caatgcttaa tctttgtaac
     7381 cattctgcta atttcagcat tgcttctctt tcgcttaaac cacacggaaa atcatcaagc
     7441 gatattgttg gcttgaagtt tcctaaatta tccatttcga cggtcagatt ttgctccagc
     7501 acggtatttc ttacgcggct attgtgccga agcaaatata ctgaacgtga tttattggtt
     7561 ttgtggtcga aagtatattc ggtaagtatc atctggcttc cgccatgatt attaccgcgc
     7621 cacataatta ctccgtgtta attgaaattt agctattaat cttcactttt atcgcgaaca
     7681 cctttaccgg tttatcaccg aagtgcggat gtgtgattgt cttgatttca tatccgtcat
     7741 acggaacgtc aattctgcga ctggaatcct cgcgcttcgg atatcccttt gtgataatca
     7801 ggcggtcata ttcccggaac ataattcgct tattccagta gtcattacac aggcgatatt
     7861 cttctgtttt ctccccgcga atcatggcat cgaagtattc acctttgacg gcaagttgca
     7921 ggttagccac ggttaacctc ctgcggcggt tccggtagcg gcatccagtg agttgcctgc
     7981 tcaataccat tacccggctt aatcgttgca tctccgcgcc gaaaggtgct tccggtatag
     8041 cgtgcggagc atattagcgg ttcaaccaga gagctatcga aattcaccga aataagcacg
     8101 ttctggccct tttcaggcat ttggtcacta cagcttatcc aactatccgg agttcccgga
     8161 gagttgccat ttacatcgaa gtttggctct gcgtcctgaa ccaggaggat gtaaccattc
     8221 ttggctgtat caagttctaa cgcctcggtg acggtaccga aatagcgatt acctaaatca
     8281 gcatcacaag tgcttacatc aatggaaact tccatccctt cgattaattc tggcaagttg
     8341 taagtttggc ttgcaggctc ggcttccagc gatgccagtg caattcgtac cagttcttcc
     8401 gcttcttctg ctggcagtac aacgttgcta cccggtccgt atgtttcgcg ccaccgcttg
     8461 attgtcaaca gtcgctcttt ggtaatagtg gtcatgccgc gtttccttct ttcttattaa
     8521 caattacgcc gtcatatatt tcattaaggt gccctctcaa ctccatgcgc cttaatgcag
     8581 ataacatgta atcgcattca acctgcttat tcccaataaa aggtttatct tcagggttac
     8641 cccaacagca attccccttg ggccacccat gtactttccg tactcttccg ttaacaacgt
     8701 gaagtaatcc ccagccaggt ggtaaatcct caactgaaat aattcccggc tcactaataa
     8761 agaatcgcca gtcgcccatt ccaagagacg gatttttacg aaaacgcttt tttctatctg
     8821 ccaacaagtc agcacgagaa catttcgcct ctatcaggca tgatgctgaa tttctgaatc
     8881 ccatagcatc tggctgttct ccggtactgg ttacagctat aaagcggtca tgaaaacaaa
     8941 ccttgaaccc gttgcgctta aggaacttat acgcaatctg acagagttcg cggtgtgtta
     9001 acgccatatc actctccttt agtgcgcaag tggtttttcc agcggttttg cgccgcgctg
     9061 ggctttttgc aaaaaccaca atccatcatc ccgtaatatt tcatcaaccc catccgtcgg
     9121 ttgctgagtc tcacccactg ccagacgcca ggagcgtttc tgcgaactaa cagaatcttt
     9181 gctttacggt ttttcatcgc tttgctctcc tgcgtctctt tgctgctcgt cgtgcctctg
     9241 caataccggt atggcggcgc tttggtgccg ggatgatgtt gtcagccatc aggacatgtg
     9301 gctttgcaat tagcgcagaa gcccaaaaac gagtcgggta cggtaacaag ccgatacatg
     9361 ccacacgcat tactcacctc ctttgatgcg aatgccagca agccagtttc ttatgccgat
     9421 atattcagcg ttcctgaaac cgctttttac atatataaaa ggcaagcgaa gattgtgacc
     9481 attggctgcc aagtagtctt tacaaccctg ctcggtgaaa cagcaggtca cgaattcatc
     9541 aatatctttc acagcaacgc gccgccattt ttctggtggc tctcgaaagt tttcgtgaag
     9601 tagttcgaga cgacgacttt ggcgtttatt ggcttcattg ccatcttcat caacccagac
     9661 aatccggtca tagtcataat cagcatcaac aacgatttcg cgcttttgat acacacaaaa
     9721 catagggtct gacgttattc gattatcctg tgttcgaata ttttcaccga tgatgccaaa
     9781 cgaatctggt gcagagtttg tctgcaactc ttcgatacgt tcagccatag cagcacactc
     9841 ttcaaagttg cttaatgctt ttcgctccca ttcagtgcat tgtttttcca gttctgcaat
     9901 tcggcacatg gcatcaatat ttgtgtcttc caggcgctta atttcggcca ggagtgccag
     9961 cgcaactttt ggtgggatag acgcgatata acgggcatta gcaccagcat tcctttgtcc
    10021 atcaatgcca ggccagtcaa taaagtatcc gcaatgccta ccctcagacg tgcgcgcagg
    10081 ataaatgccg ttatgacccg gcaaaatata tgctacccat tcatcttgcg ttgcctgttc
    10141 cgccgcctcg cgcagttctt gatagttaat tttgatcact ggttgcctcc gcttcccacg
    10201 ttttcagact ttcaccacag aacgggcaaa atgaaattcg aatcggcgat ttagaaaact
    10261 cgcctgaccg cagcatgatc aggtcttgtg aatgaattaa ttcatggtta tagattttgt
    10321 atttcagcag acctttccgc gtcgtgtatt cagcatcctg ctccagggat tgtgccaacg
    10381 ccgcgcacgg ttctattttt ttgccattaa tcaaacaagt ggagattctg cactttgcag
    10441 ctccgtcacc attaccttga ctcattgcat tgttcccatc agttattgaa cgtgatcact
    10501 ccgcgctcga tggcgaaatc gaaaagctgg ttagcggcta cgtaaaggcg tatgccatgc
    10561 gttttttccc atgcccggac atcgttttct gcgcttctgg cgcattcatc gcaaagagga
    10621 acagcccagc gatcgtgttc gtttaacgag cgggcgcggt acatgaacgg gtgattaact
    10681 tttccaccgc atccgatgca cggacgagaa accacaaacc ggagataagc cgggcttttt
    10741 ccgagtaagg tttttggacg gcgcatatat aggaggccag aatcttcatc gacggacaga
    10801 ttgacgatct gctctgcgga aatatccacc agctcgcggg tactatgctc ccaggtaata
    10861 tccgactctt tcagagtgcc ggtagggatt tcagtttttg gctgacaaaa tgcaatgcga
    10921 ccagcttcat caggcagttc gtctttaaga ttccggcgaa tggcccaaaa ggtgagttca
    10981 accatgctca gatcgcgatc tggcggaagt tttaactcgc tggcagccca gttcataatc
    11041 cagttggcgc gattccgtga cagttggtct tccagcttgc cgtatccctt catcatgtat
    11101 tccgcatcat gcttccagca caggcgaacg gcagaaccgt tatagaaatg ggtggttagc
    11161 tggtggctgc aatcgcgctt atcatgcgcc tggcaatcgt gaatgttggt gctaacccag
    11221 tgaacgagag aatcctcacc acctaacgcg ttaaatacac gctcactttg aaaaaagggg
    11281 gccagtgact ggttggcaac cagtgaataa ttcaggtcaa cgacaccatc aggggtgttt
    11341 tcagcctgtt cacgcggcaa tggggatata agaaaacggc gtcctgcgcc gatgtaattg
    11401 ctggtgggct tatctacagg aaagacagcc acaccagttt catttacgac atatggggtg
    11461 attattgcac tcataatgca taccgactct gttttgttcc gagcggtaaa ataatatgtt
    11521 agaaaactaa aatcaatgtt ctaacgtaat tttgagtgcg taaatgattt gatcagactt
    11581 taagcttccc tcccttcacc tgcattagcg tcagatttcc gcaaaaaacc gcgcccgtgt
    11641 ctatgtagtg ctgattccag tatgcttttg gtcttcgtac cggtgtgtgc ccaaagataa
    11701 agcgatctgc gcctgtgatt tctccaccaa tgccatctat cgaatcaccg acgcgactgc
    11761 gcgaccagac aacatcaaaa agcgacacat ccttaccgaa ctggtactct ccacctggat
    11821 agtcggcatg ggctataacg atagtttcat gcccggtgtt caactcaatg atatatggca
    11881 gacgctttac cagctccacc agcgccttgg ctaatatttc ctgatcagtg cccagcatga
    11941 agaaccattg tccgccattc attagccagt tattcacgtt gccatcagga cttaacgcat
    12001 caagcatcaa ccgctcatgg ttccccatta ctgccctgaa ccagggcatc tgcaatagtt
    12061 ccagacattc gacattttca gtaccgcgat cgataaggtc gccaaccgat atcagtaaat
    12121 cctgcgccgg gtcaaaatcc acacgatgga gttcggacat cagtctggtg tagcaaccat
    12181 gcagatcacc aacaacccat atgtttctgt agccagaacc atcaatacgg ctatataaat
    12241 tcacttcatg catagtctgg atcatgcggc aaccttctcc cgcagccaga tacaaactgg
    12301 accatcttca gtgtcatgaa tagagccaac aaaccagcct tcaccctctg gtcgctccgg
    12361 ttcccaggcg gcaatatcgg gaccagctgc gtccagatca aaatcatctt catccatact
    12421 gcgaatagtc cactgaagat tattttcctt catccaggcg tcaaattcct cagtggaaat
    12481 atattcacga cctgcgcaaa acttctcata ctccggatgt gtccagcagc catattcatc
    12541 acgttctacc ggcatttctt taatgatgct cactcttcat cctccaagtc ggcaacggcc
    12601 tccatcacat cagaaccgcg aataacctca aaagcacggc aggccatttc aaacacctgt
    12661 tgttcttgcg gatgcggtga ctcccaatac ttaaaaccag gtcgatgctc gtaacccatc
    12721 atggaataaa aatcgccagc aagttcaatc gcggcatcaa caagttcgcg atttgtcatc
    12781 gtctgttctg tcatttggtt ttctcctgtc tgaacatcac tatcatcagg tcgccttttg
    12841 tcgctattct ggctgttgtg cctggttcaa tgcggctaag ctcaaatgcg tcatagaacg
    12901 cttctaatgc cttctggcgt agttcctgtt tgcgccgttt tttccactct tttaggaaaa
    12961 tggaacccag ccatcgccag gtacgggaca tgatgtaaag ccaaccgaaa agtgccagac
    13021 cggtatttag ggccgtatcg attgttatcg tggtgtcgat attcactgtg gtggctcctg
    13081 cttttctgcc ttcaatacca tgcgagaacc atcatccagc tcccaattaa tttcaccgcc
    13141 ttcagccatg actagatgcc aaacgagttg tgcggcctcg ttggttacat cacgaccgcg
    13201 atcattgccg acacgacgtt ttgttccatc ccctaagtca cgcatttttg ccaacacgat
    13261 ggtttttgat agcggtgaaa aaccaagctg tagtcgtgcg gaattactca ctgtttgcct
    13321 ccttttccaa gctgggcggc gatatcttcg agaacgccat cagagaatga gcggtcaaaa
    13381 tcgccttccg gcgcattagc cataaactca gtagaggtaa gaatcatccg ggcaatatcc
    13441 gcggcgttct tcgcagtatc atcaataaaa ccagcttccc aggcagccag cattctgttc
    13501 gccacaaagt aagcgccctc cttgcgtgct tcagtcttca cttcagccag gaaagcatcg
    13561 gtggctggtg tttgcggaat ccctccatct gttgcgcaga tatacgcatc agatatttca
    13621 tcctgctcgc catcaaacac gtagcaactc tgtgcgatga atttattcag ccccgcattc
    13681 tccgccgcca gcgccgcgcg attaccctcc agctctgcaa tgcgctgttt tgcggtatca
    13741 agttcaatcg ataatttttc caactgctct ttatgcttct tgtattcctg atatgcgtgc
    13801 caggactgac ctttgcgcac actatcagta atatcagtaa tctgttctgg tgttagcgta
    13861 gtcagtggct gtgctgggaa aatcagcact ttcccggaat cccaatcaaa accagcgtga
    13921 attgactgaa cctcaactga gggtgttgaa ccaatgctgc caggcgaatg aacaacgatc
    13981 gttacatcca tatcgcgacg atggctgtgg ttgttggaca aaatacgatt caccaactca
    14041 gaaaatttgg agaatttcat gcggagcctc aatatgcaaa atagaccgtt gccacgccgt
    14101 tatagtgatc aaacgatacg gcgtttactt catagctggc agggagcttc gagccgagaa
    14161 catatccggg ccacgttttc catggaattt ctctactctc actatcccca tataccgtac
    14221 atccaagtga acctacagcc tcgtcagaac gtttgccgca tgttatgaaa cctatatttt
    14281 tcttgccggt cttaatggcg attgggtgaa cactggctga tgcattggca gcacgctgtg
    14341 actgttggtt tgcgatattc gcagagttcg cagcagctac agcagccgtg gtcgcggcgg
    14401 tagatgccac aataactgct gaagcctgcg cctgttgggt aatggtcagc aatgccgcca
    14461 caaaaatcat cctcttcact tgccagctcc tttgcgaatc tgttccgccc attcttcaag
    14521 ggatttctcc gcatattcac cagacaggcc atcaatcgga tgcgcttcat tagccaactc
    14581 ttctttcgct gacaaaatca tgcgtgtaac gtcgaaaact tcacgcaaag acttattgat
    14641 aaatccgtga ttgaacgcag cagcaagacg gcttgcggta tagttaatcc cctcgttgcg
    14701 agccgccgca cgaatttcag ccagaaaagc atcggtggct ggagtttcgc tgtggtgcag
    14761 ggcatcgttg ataatcattg cagcaactcc ggcctgccct gcatccgtga ccgacacatg
    14821 ctcaagagtt acagccattg catgtttcag cccagcattc tccgccgcta gcgcagcaca
    14881 cgctttctca agtcgggaaa tttccgttac atactcagcg ttacgctctg ccagttgtgc
    14941 gggcgttaaa ccttcagact tcatacactc tcctttcgaa ataaacgtac tgattaatca
    15001 tgcccagggg catttcgagt ttttccgcga tctcacggcg gggaacacca cactgatgaa
    15061 gctgccgtgt cagttctata tcgctctgcc ggtatttggc tgacggatga aagtctcctt
    15121 tcagaatcat gctgatcccc aactcccgcg ctttagtcct gacggcatca ccggtacgac
    15181 cgatcagctt cccaatactc tcgaccgtca tagttccggc gcactggcgg agtatcatga
    15241 tttctgccct tccccatccc cgccagctca cgccgacatc ctctgagaca taaactccac
    15301 aaaacgaact gcatcttcag aaacaacgcc gcatgacttc agataattca ttgcgtcatt
    15361 aggtagcgga ttagttggtt cagcatttgc cagggattga gataaccgcg tgattaatct
    15421 gaccattctt cgctgtcgga ggttgtatcc ttcagccaaa gacaggtaac tggaaagggt
    15481 ttggcttaag cgagcgcgaa gctgatccgt gccgtgacat tcaatagcaa catcacgtaa
    15541 cgcattgacc agttcccggt aaatatgcgt agcaacctgg cattcaatgt tggttggtga
    15601 ctcataaatt acagtcagcc caagcttttc agccaacgcg tgctctgcac gagcaccaac
    15661 cgattcttcc cattgattga gcaaatagat cgcatcagca gaacgcacca ttgccaggca
    15721 aatatccatg tattcactct ggcaaaggcc accaggtaac accgctggat tcagaacgat
    15781 atgcccttca gccatcaact cgctcgcttt cgcattaaac gccgcacggt tgtaatctgg
    15841 ataaccagtc atcggccccg cgatgtaaat tttcaaaaca cgtttcacca tcaaaatttc
    15901 cccaaaatcg tgacgcaaaa cataagcact gcgaaaagct caataccaag ctcaagtagc
    15961 gccaacgccc cgaaaaacaa cacatagaac aggccaatct cattcacaga tggctggtgg
    16021 atcgcactta aagctataag catcatgtcc tccaaacagt tatcaggaat gatattaatg
    16081 ttttgtgtta gaaaatcaat tttgaatatc taacaatttt gatcaatcat agaaaaatcg
    16141 ctgaaaagta agaggcagcc atcatgcaga aacagagcta ctgggagaaa cagagctact
    16201 gggagaaaca gagacaaaaa gccatgcaaa aattggctga cccggcctgg cgagaggaac
    16261 aaagggcaaa gcgccttcaa caagctcaac gccagcagca gcgagcgaga gaaaaagccg
    16321 catcgcctga atatcggcaa aaaaaaattg aaaaagcaaa gcaatatgac cagcggagaa
    16381 aagagaaagc tgtatccgct ccgtccaaaa aaacacgcac gtcacgcggc ctgaagggca
    16441 gatcactcac agccgatgaa cgccggatac agaccgctat cggtactctc ccctgcattg
    16501 cctgccatat tcacgggcaa catagcccag tagtatccct gcaccatatc ttcgggagaa
    16561 cggcagagaa cgcgcataaa tatgtcctcc ctttgtgtaa atggcaccac cagtacgcag
    16621 cgccagccga ggtccgagaa caatatccct ggctggtccc tgttcatgct gatggaaaaa
    16681 taggcggaaa agcagatttc atgcggcaca atgccgatga aatggcgttg tatcagatgg
    16741 caattgaatt gataaattag ttttctaaca ttttaagttg aattgaaaat ttctccgatg
    16801 tacattcact accgattggc acaccggtca actttttgag acaaactgtt tagtttttcg
    16861 tattattgcc gccgccatac ctatggcggt gcaatatagg tggctgaaaa gcccccgttg
    16921 actcacggtg ttccagcctt tattgcgccg ccaccagacc gtggaacagt cgatggcggc
    16981 tccgaaagca aggagtcact acactatgaa caactacagt cttttcccat cactcgtcgt
    17041 ccaccactca cgcgataatc tgcattcttt actcgcgctg ggggtgtcaa aatgactgtt
    17101 cgctacctca actttcaaat caagaacatt actggcggtt gctatgactg gtttgtcact
    17161 ctcggaaaag aagtgatcac cgggaagctg gatgaagtga aagctaaagc aatggcctac
    17221 gcctgcaagc aagcccggaa aaaatccgcc aaagcataaa atactgactg ttgcgccctg
    17281 gcattatcgt ggggtatatt tctacggcac cttagaaaaa cgggtgccgg gattgagacc
    17341 ccggataatg caaaaggcga cacagacgcc gaaagcgtct ttttttgtgt catgccatcg
    17401 cacagccata cgtagcgtat agctccaaga tcaatggtag tgctggctgg gctgccgaaa
    17461 ggcaggccgg ttcccttttg cgccggtagt ctcaacccag ccagtgctac cgccattgag
    17521 attgagacct cacgcggtag ctccttaaat tagcaaaagg aggctgccaa tatggctact
    17581 atccctaccc caactcattc tgaatttatc tggcgtttct attcctgcca aaagcacctg
    17641 tatatctgcg tcatggctgc taccgaatca gaggcgcgct cataccttcc cgaagaaccc
    17701 tgcatttttg ctgctcgctt cactcttgat gcgatggaga tcctcaatta ctggaatctg
    17761 ccgatgaact gcgtggaggt gcactgatga atctgtccat ctctcaaaaa gcgacaatga
    17821 gtagcctaga tattgcagag ttagttggta gtcgtcacgg caatgtttta cgtaccattc
    17881 gcaatatgat ggctagcggt gtcatccgag aaactcaaaa tgagttcgtc gaaagaatca
    17941 ataacttagg gaaggttgtc aaagatccgg tctatgtctt cgaaggcgag caaggaaaac
    18001 gagacagcat tgttgtcgtc gcgcagctct ctcccgaatt taccgcccgg ctagtggacc
    18061 gctggcgcga actggagaat gcccgggtac agttaaaatc aaaagccgaa atcctggctg
    18121 aaatggcgca aatgcatctt gagcatgaac gccggatcaa cgccgtaaat gcccaggtag
    18181 ccgaggtatc agcacaagta tcgatggttg ctgaaacact cgagcaaatc aagaaaggca
    18241 atatgccgga aggttacatt ggctaccgcc agctggcggc gaaatgcggc ctgactgaag
    18301 ccaaatgccg caacctggtt aacgcttacc ggattcccac cgatacacat gagtttttga
    18361 cccccgatgg cttgcttgcg cgacgctcca ttgtggctct ggcccccttc cggaaagcct
    18421 ttaagcaggt gatgtcggag gcagaaccac gcaataaacg ctggtatcat ccaaagatgg
    18481 ggatgtttca ggcaatccac catcctgtgc ctgaaagtcc aaaggcaaac ctgtcgttgc
    18541 ataccgccag agagaggatt aaaacagggt atgcaatcgt atgccgtcga gcatcttggc
    18601 ctgaaggtgt atgggtatgg cccgaaggtg gatcacgaaa gcactggcgc actatccggg
    18661 atgggaaaat ccatgcgatt gatttagctc cagaggatgt tgttgctacg gactggattg
    18721 ttagttaatt actgttgccc cggcctgtcc ggggctttaa ctactaaata accggattct
    18781 ccgcacgatg aagaaaatag atttcactta ctctgctgcc acaatccagc gacgtttcag
    18841 tctcataagg gaagtggaac tatcaaaaaa ctggtatcaa attctactgg atgaagagtt
    18901 ttcactgatg gttatagcag aaaaattggc tatgccgaat gaccggcaca aggtcatagc
    18961 cagcctggat ctggtgacaa acagatactg ggaatccgag gaactacttg aagttggctt
    19021 gatacgcgag atgatagaac aggctgttcc cctccactta cagcaaccat aattgaaagg
    19081 tcttaacgat agtctgccac gaatagttac tttcactgcc taaagttatt taattgcatt
    19141 acaaagtgta aattagtaac attagaatct cacacagcac tataactata tgttttttat
    19201 gtaaaaggat gataatcgtg gctactaaaa ttaaacttga aaaccctcaa actggcgaat
    19261 caatcaccgg tttttatggt ttttcctgga ctacattctt gtttggtgct tttccagctt
    19321 tgttcaggaa agactttata acttttattg gggtattcgt agtgatgctg atactggcat
    19381 tcctcacagc cggaattggc ccgtggattg cctcatttat ctgggcgttc atgtataata
    19441 aatatttcac cgtaaataag ataaaacaag gcttcatttt tgctggttct cacacagaaa
    19501 atgagttggc tgctagcaag ctaggattgt cattaaatca aaacaactgc aaaactatta
    19561 ccgagtaata gccaaaaagc aggcttaata gtaagcctgc tatgtcaatc acgaatctct
    19621 ctgacaattc agaaacataa acgcccgctt gtctgttaaa tccgtaacat aatactctct
    19681 catgcctgta ggttttattc tacgagcata tacaaactta tgaactggtt gtttttcccc
    19741 ataacttaca tcagtcctaa attcatcaac cacgctacca tctttttcga cttccttcct
    19801 caccagctta ttttctaatg ctggctttaa ttcgtccatg ccactaaaag catcctggcc
    19861 tgtactattg cctcgatttc cgcgcggcaa atcccaggag aaataagtgc catagtcagt
    19921 aatcacacga tcccagactc cggtatcccc catatagtta gagcgctttc cattgtaggt
    19981 tacagccact ttacaggagt acgtttcttc attccctcca gccttactcg ctgtggttgc
    20041 agggatattt tggatagatg cgcttacgtc atcttgcgtt aattcctcaa ctggagcaaa
    20101 ttcatatatt ggtgtgccga ggctatttaa atataactta tatctaaccc taagcgcact
    20161 tccatcagag aattcatacg ttcccagaca ttggagctta tttttttctc ttatctcatt
    20221 gatctgatta taagcatcaa ttacagagat tgcttcctga tcgtccttca gcagttcatt
    20281 ataatcatcg attaacatct gtctggtttt aggggcatta cagtcaattt tgctaaaatc
    20341 ataagcaaag gcaaatgtgg gaacagagag agcaacaaca gtagcaagta ttgttgagga
    20401 acatcttttt ccatttttta taaaaaacat ttcattaact ccatacttta ccatcttagc
    20461 cgcgagcgcg gtccctaaaa cccttaactt cactacaccg tccttcatgt aaacaaaatg
    20521 aggttttatg ttataaaaac aatcaactac atcaacatga tttaggccaa gtttcggtca
    20581 aataataaaa tcctcaaaaa gtggttgaca ctattctgga aatcacaaac tgcacataat
    20641 ccatcgcgct aacggctccc gttggaggtt cttttgacga ttaactttca gccgaagcgc
    20701 ggtagggagt cataacgcca aagcaggccg ccatgtgcgg cctttttttg tatccgtcat
    20761 ccgtggagga aggacaatgg agaagatagc aatattcagc ctgaccacca gcaaaccgca
    20821 aatgctcact gcaatactga aagacggtgc tctcgtgatt aacgaagtaa aaccccttcc
    20881 cgcgtcagca ttggaacaaa agcaaaagat tcctccagct atagcagccc tgcggaaaag
    20941 caaatttaag gtgctggtag acgaaattac gccaacgatc tccgctggca ccggagcaag
    21001 ccaggtaaca ctcaagacac gtcatgccga cggcagagct gcgatcatcg tagggatgga
    21061 aagatacaga gagctaaaac tccagaagct attatccctg ccgcaaaaca ataaaggtgc
    21121 tttcgaaatt cccgactcca tcgttgacac cgaatacaac ggtaacggag aagaagttta
    21181 ccgggtgaac tggcaggata tcaggccgga acatattttg atgatcctgt gttgctacgc
    21241 gaccgtatac cacaatgttg ccagtgcgga ttacgtagag cagatgaccg gtacagtcga
    21301 gaaagagcaa aaaacaggca tactcgcttc gttcctgtcc attattgggc atgaaaaagt
    21361 taaagcaggc acctcccagc caaagtcgct gactgggaaa gaagttgatg aagataccgt
    21421 gatactttga tcacattagt agctttgtta actccctcaa taccaggtag aagagtaaca
    21481 aaaatattga cgcgagtatt tgagtggtca atgaagcgtt cataaacaga tcaattagca
    21541 ttaataattc gaagataaca tccatgtcat ttactccgtt tgatttttaa tctaatgcca
    21601 gcaaatgaag ctggcccccg cataaagatt aatgaaagtc acttgtcacc agtgagggga
    21661 tttatgaacc ccatccccct gaactggtgg cctgtctgtt tctcgcattt cacaccctgc
    21721 atacagtttc ccccaggtta tgactgagag gctttgttat gggctatagc agactcgacg
    21781 ataggtacat tgaagacgat atttttcgtg cgctgtttca ccaggaaatg attaagcggg
    21841 tatcggagta tcactctgat aatttccagt acacgataaa gattgatgaa gtatatcgtt
    21901 cagaccttgc agcctacagg gcgtatggca atgcagattt gcgctgggta ttccgggtgc
    21961 tggtgggcca tgagtcagaa atggaagaaa tgcccgcagg gaccacgtta actcttcctg
    22021 atgtggcatg gctgaggaac aagatccgtg attacgcaag cgcggaaccg gagatagaaa
    22081 atgcctgatt tcctgaaaaa ccaggacggg cgctatatca ctgacggcct gtcctctaag
    22141 gacttcacgc gtttattcga ccttatcagg aaagaacaaa cccgtaagcg ccgacaagct
    22201 caccggacgc tgacgccagg tagactgagg aacaaatccg ccgaagatat tctcaagtta
    22261 gggaagaaaa aaggcggcac gttcttcacg cgagacgacc tgaaaggttt cgaaaagcta
    22321 cggagtaaaa cgcgcgaaaa atatgacagc aagacggctg gcatcacata cgcccagctg
    22381 gtggcatcca gccaggcaat cgatattaag cgtgcaaata acgccgtgga tgacggatct
    22441 ggtatcaaaa gagctacacc cgtatctctt cgccacaatg tgattaatat ccgcgtagaa
    22501 gcatcggata tatccgtcca ccagcaccat atcgtccgga tacgctttga agaatgggat
    22561 cagatggttg atgacatcgc agaagacgat aaatcagctc tcaaaataac taaatcactg
    22621 tgcgccgggc gagtgtcttt cgattgtgac tgtggtcgtc atcaatactg gtatcgttac
    22681 atcgccactg cgggtaactt tgccctggca ccgccaaaag aatacgccta tccaaaagtt
    22741 cgcaacccta agctgcaagg tgtcgcctgt aaacacgtga ttcacgcaat gacacgacta
    22801 cagtccgcca gttggcaaat gagcattgct cgtgcgctac aaaaggctgc aacgcaaatt
    22861 gcatttggtg acgatcgccg ccgtacaacc aaacacttct caaaagaaga cgagaaggag
    22921 tttaatcgca atcgtagcag taaaacgaac gttgacgctg ccaagcgcga atggaagctc
    22981 tatcagaagc gccaggccgc tttaagtgaa aaactggcga aggacaacgg caagaacgac
    23041 aagctacgtg accaactgac cagggccaga aagttgtcag acgcacagaa aaaacgagcg
    23101 gcagcaaaag aagcggcctt gcaacgtgag aaacagaaaa acaaggagct tcagcaacgc
    23161 cttgccgatc agttcgcact gaagaagcag gcgttcattg atgcgcttgt catggctgga
    23221 acaccaccag cacaagctga aaaaatgttc atggagtatg taaaaaaagc ataaaccaca
    23281 catagcctag tagtttatgc tgggctttca tttttttgaa agatcaattc cagtattatc
    23341 aaacaaactg gaaatatcag acaccaattt atta
//
LOCUS       DAZFWP020000074        23357 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFWP020000074 DAZFWP020000000
VERSION     DAZFWP020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.074"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            52..471
                     /locus_tag="ACHK0Z_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             52..471
                     /locus_tag="ACHK0Z_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HGU4502723.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            468..1028
                     /locus_tag="ACHK0Z_RS13630"
                     /old_locus_tag="pgaptmp_002726"
     CDS             468..1028
                     /locus_tag="ACHK0Z_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HGU4502724.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1029..1274
                     /locus_tag="ACHK0Z_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             1029..1274
                     /locus_tag="ACHK0Z_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGU4502725.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1271..2773
                     /locus_tag="ACHK0Z_RS13640"
                     /old_locus_tag="pgaptmp_002728"
     CDS             1271..2773
                     /locus_tag="ACHK0Z_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4502726.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2782..3147
                     /locus_tag="ACHK0Z_RS13645"
                     /old_locus_tag="pgaptmp_002729"
     CDS             2782..3147
                     /locus_tag="ACHK0Z_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4502727.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASASSTRVQ"
     gene            3162..3638
                     /locus_tag="ACHK0Z_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     CDS             3162..3638
                     /locus_tag="ACHK0Z_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4502728.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3765..5840
                     /locus_tag="ACHK0Z_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             3765..5840
                     /locus_tag="ACHK0Z_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4502729.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            5827..7176
                     /locus_tag="ACHK0Z_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             5827..7176
                     /locus_tag="ACHK0Z_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HGU4502730.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAFSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            7160..8284
                     /locus_tag="ACHK0Z_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     CDS             7160..8284
                     /locus_tag="ACHK0Z_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4502731.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            8274..8888
                     /locus_tag="ACHK0Z_RS13670"
                     /old_locus_tag="pgaptmp_002734"
     CDS             8274..8888
                     /locus_tag="ACHK0Z_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4502732.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            8881..9318
                     /locus_tag="ACHK0Z_RS13675"
                     /old_locus_tag="pgaptmp_002735"
     CDS             8881..9318
                     /locus_tag="ACHK0Z_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4502733.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            9318..10400
                     /locus_tag="ACHK0Z_RS13680"
                     /old_locus_tag="pgaptmp_002736"
     CDS             9318..10400
                     /locus_tag="ACHK0Z_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4502734.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            10391..10951
                     /locus_tag="ACHK0Z_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             10391..10951
                     /locus_tag="ACHK0Z_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGU4502735.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            10951..11862
                     /locus_tag="ACHK0Z_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     CDS             10951..11862
                     /locus_tag="ACHK0Z_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4502736.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(11897..12418)
                     /locus_tag="ACHK0Z_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     CDS             complement(11897..12418)
                     /locus_tag="ACHK0Z_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4502737.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(12498..12701)
                     /locus_tag="ACHK0Z_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     CDS             complement(12498..12701)
                     /locus_tag="ACHK0Z_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4502738.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            12730..12909
                     /locus_tag="ACHK0Z_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     CDS             12730..12909
                     /locus_tag="ACHK0Z_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502739.1"
                     /translation="MSCVPSNGTPLIFLGVASLFAATAAPFDGRAFAALFTVSRKPRF
                     QGCRISALKGLSTSF"
     gene            12923..13483
                     /locus_tag="ACHK0Z_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     CDS             12923..13483
                     /locus_tag="ACHK0Z_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4502740.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            13583..15622
                     /locus_tag="ACHK0Z_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     CDS             13583..15622
                     /locus_tag="ACHK0Z_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4502741.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENDANVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMGLYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            15769..15951
                     /locus_tag="ACHK0Z_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     CDS             15769..15951
                     /locus_tag="ACHK0Z_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4502742.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            15987..16232
                     /locus_tag="ACHK0Z_RS13725"
                     /old_locus_tag="pgaptmp_002745"
     CDS             15987..16232
                     /locus_tag="ACHK0Z_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4502743.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(16271..16735)
                     /locus_tag="ACHK0Z_RS13730"
                     /old_locus_tag="pgaptmp_002746"
     CDS             complement(16271..16735)
                     /locus_tag="ACHK0Z_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502744.1"
                     /translation="MIQGGDYLLKENEELGGENDHKMIILLTFYTVKVGSRVYVVCFK
                     STGYYIKVKPSIAGTMLGQSVKTCINALLRARGVSYDIPRSPVKQRCIKRRSLSWMKE
                     PVCPACKTPLQLIYRRSRSQNGKTYKPKNASCFRMLICPACSPRRENTQNVH"
     gene            16850..17050
                     /locus_tag="ACHK0Z_RS13735"
                     /old_locus_tag="pgaptmp_002747"
     CDS             16850..17050
                     /locus_tag="ACHK0Z_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4502745.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            17004..17741
                     /locus_tag="ACHK0Z_RS13740"
                     /old_locus_tag="pgaptmp_002748"
     CDS             17004..17741
                     /locus_tag="ACHK0Z_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4502746.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            17869..18621
                     /gene="ompN"
                     /locus_tag="ACHK0Z_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /pseudo
     CDS             17869..18621
                     /gene="ompN"
                     /locus_tag="ACHK0Z_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            18762..19196
                     /gene="uspF"
                     /locus_tag="ACHK0Z_RS13750"
                     /old_locus_tag="pgaptmp_002750"
     CDS             18762..19196
                     /gene="uspF"
                     /locus_tag="ACHK0Z_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU4502747.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            19372..20307
                     /gene="ttcA"
                     /locus_tag="ACHK0Z_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     CDS             19372..20307
                     /gene="ttcA"
                     /locus_tag="ACHK0Z_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU4502748.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(20436..21809)
                     /gene="dbpA"
                     /locus_tag="ACHK0Z_RS13760"
                     /old_locus_tag="pgaptmp_002752"
     CDS             complement(20436..21809)
                     /gene="dbpA"
                     /locus_tag="ACHK0Z_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU4502749.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(21839..22012)
                     /gene="ynaL"
                     /locus_tag="ACHK0Z_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     CDS             complement(21839..22012)
                     /gene="ynaL"
                     /locus_tag="ACHK0Z_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU4502750.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(22287..23270)
                     /gene="zntB"
                     /locus_tag="ACHK0Z_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     CDS             complement(22287..23270)
                     /gene="zntB"
                     /locus_tag="ACHK0Z_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU4502751.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(23284..23331)
                     /gene="ydaN"
                     /locus_tag="ACHK0Z_RS13775"
                     /old_locus_tag="pgaptmp_002755"
     CDS             complement(23284..23331)
                     /gene="ydaN"
                     /locus_tag="ACHK0Z_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU4502752.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         5686 a         5834 c         6431 g         5406 t
ORIGIN      
        1 acaggatgct ccccggaaaa agaccggtaa caaagcggaa caggcccgtg catgaattac
       61 gccactgaaa ccgatatgcg ggcgcgttac cgcgaggatt tgctcaggcc gttactggct
      121 gtgccccgtt cggatgagcc ggacacgcgc aagctgaacc gggcgctgac agatgcgtca
      181 gcccttatcg acagctatct gtccgcccgt tacacgttgc cgctggaggt tattccggcc
      241 gttcttgttc agcactgttg cgcgattgcc ttttattacc tgtgcgatca gcgagcctcc
      301 gatcaggcgc gtgaccgtta ccgtgaagcg ctggcctggc tgaaagatgt catgaacggc
      361 aatgtgccgg tcggtgtgga tacgaacggt gcggcccctg aatccgggga tttaccacag
      421 gttcagtctg atgcagcggt attcgggcgc aaccagaagg gcttcatatg attacggaaa
      481 ccgaacaggc atacatcgcc cgtatccgtg agtatttcgg gaatgaactg gtgtctgttg
      541 acacgcatcc cggcgactgg agcgacagcg tactgcgcac catgctgatt aacgccccgg
      601 cgatctacgt tgcctggctg ggtgccggtg aaggccgtac ccgtggtcgc ctggtcagtc
      661 actgggtgtt ctacgtcatc ggcgacatgc tcaacgggcg tgaggccagc cgtcccggac
      721 tgtatcagat tgtggcccgg ctgattgccg tgcttaacgg cttcagaaca gaaaaaacct
      781 caccgcttta ctttgaaaag gcggtcaacg gttacaccga aacccaggcg gacagtggtg
      841 cggtgatgta tgcgctttat ttctcctgcg aggaaatgat cgcaccgctg accgatacca
      901 gctcgctgga cgacttcctg cgtcattacg aaacctttgt ggagccgccc ggtacgccgc
      961 cgtttgaggc acatattgat ttgccgggga acacgacaac ggcggtcgaa ccgccggagg
     1021 aactgtaaat gaaaaccatc tttatcaaac ccgcgccggg acgcctgatt cgtgatcccg
     1081 acacaatgcg cccgctggca caggaggggg aggaaaagcc ctttacgccg ttctggtgtc
     1141 ggcgtcttga tgacggcgat gtcattcagg ctgaaaaagc cgctgaagag gctccggctg
     1201 tatctgcgga tgcaacgact gcgacagaaa aaccggtcgc ccaaccggca tccgataagg
     1261 agaaaccgca atgattaatt ttgacgggat cggtaatgac cgccgtatac cgctgattga
     1321 ggttgagttt aataactcca tggctgtcac cggcactccg gcacagcgtc agtgtgtgct
     1381 gctgtttggt caggcgagaa tgaaagagaa tgccgtggac ggtgccggtg ttctggatgt
     1441 tccggtcagg atcacccgtg cgtctcaggc aacagaactc tgggggcgcg gctccatgat
     1501 tgccctgatg gtggcggaat ttatcgccat caatcctgat gctgaactgt atgccattgc
     1561 tcaggggaac ggcaccggac aggcaacggc tgcggccatg aatctgaccg gaacggtcac
     1621 ccgtgacggg attgtttatg cctgcgtggg ggggcggcgt tatacactcc cggcaccaaa
     1681 aggtaagaaa ggcaaagaac tgaccgacga gctggccgcg ctgatcaatg ccgatcctga
     1741 cgcgcccttc acggcctcct ctggtgcagg aagtggtgat aatggcgcag gtctgaaagg
     1801 gtctctgggg atcaccgccc gctttaccgg tgaatgctcc gttcatgatg tgcgcctgaa
     1861 ctatcacgac ggggaagcaa ccccggaagg aatacaggtc gctatagcct atccgaaaca
     1921 aaaagcggct aacccggata tcacccgaag cgtggcgggt atgggcgatc gccagtacaa
     1981 ctatgtcgtc atgccctata aggatgatgc aaacctgaaa atcatcagtg atgagcttct
     2041 gaaacgctgg ggaccggcca aaatgtctga cggcgtgctg tggctggcac ataccggcac
     2101 gtttggtgag gtgcaggcat tcggggcaaa acgcaatgat tttctggcga cctgcacatc
     2161 cattcccaaa gcccctgaac cggattacct ctgggcagcg gcggtctgtg ccacctgtgc
     2221 accctctctg ggagcagatc ctgcgcgtcc gcttcagacg ctggcgcttc cgtcacgcat
     2281 ggccccggct ccggccgatc gtctgacgcg tgaagagcgt aacagtctgc tgtggggagg
     2341 gattgccacg gtcagtgtgg ctgccggtga tgtggttcag atcgaacgtc aggtgaccat
     2401 gtatcgccag aacgtgtacg gcgaaagtga tcccagctat ctggatgtgg aaaccatcta
     2461 caccctctcc tggctgcgtt attccctgcg tacttttatc acacaacgtt ttccgcgcca
     2521 taaactggct gatgacggga cgcccgttcg tgccggtcag aatatcgtga cgccggagat
     2581 catgaagctt cagttaattt cgctgggtga agagtgggtg gaactcgggc tggtcgaaaa
     2641 tctggacacc tttaaaaaga atctgcttgt ggagcgtaat gcgtcagatc gaaaccgtct
     2701 tgacgtgtta tgcacgccgg atctggttaa ccagttccgc ttcctggcag cacagatccg
     2761 tttcattttg tgaggtaagg catgagcgga aaacagtatc agggtacggc cactatccgt
     2821 gtgaacggtc aggaatacga gacgcttgag ggggctacat tttctccgtc tggttttgaa
     2881 cgtgaagtgg tgaaaggcgc gaaagtctat ggttaccgcc agaaaccacg tgaagcgacg
     2941 ctggactgca aatttccggc aggcggagaa ggttcaccag ccgccgatga aatcaacaac
     3001 tggactgcgg tcactatcga gtttgtggcg gataccggcg aagtccacat gatgacgaag
     3061 gcctggagca gtgaaccggc ctcgcttgac ggtggcgggg agatctccgt gaagttcgcc
     3121 agcgcctcca gtacccgtgt tcagtgatca ggaaaaataa aatgaccaca cgtaagaaaa
     3181 aaacggcagt ttccgaggca gccgtgatgg aggcaatccg ggaagcactt gagggggctg
     3241 atccacgcac tgccgggctg acagagcaac tggcaaaggg atatgtggat ctgcttgatg
     3301 gtctgccgtt tggtgagacc cgtgaatatc gtgtcacgtt ccgggaactg acagcgaaag
     3361 acagtattga cgcagaagca gaagccgagc gcgtggtgga gacaaacaat ggcccgatgc
     3421 tgatagcgtc tccgtcgtta cgcggtgttg cactgctgcg ccgtcagatt gccgcagtgg
     3481 gagacattga aggtccgttg tcaccccgtc agattggaca gttaagcgag cgcgatctct
     3541 cccgcctgat ggcagcggtc agcctgctgg ataccgcgct tgccggaaag ctggcagctg
     3601 accgggggcg atcaggcgca gtgtcgggat cagattgaag aagcggcaat tatcctgggg
     3661 atggtgacaa aaagcggtcc ggaatgggcg cttaacctcc ccctgtcgca actttaccgg
     3721 cactgccgac agacagaaaa aatcatcaga acgaagcagt aagtatggcc cgaaatctca
     3781 gagcatctct gatagttgat ttgctcggca atatctcagc caaatcccgc cagtggtcac
     3841 aggagctggg ggcattctca cgctccggcc aggcggggct gggtggcctg ggaaatgctg
     3901 cccgccgtgc cgggcaggaa acagatgtac tcggaagcag gatgcagcgc acgctggccg
     3961 gggtgcgggg cagtatccgc catgtcactt ctgattttga ccgtcttcag ggcagtatta
     4021 ccggcaccat cgggcggatc agcaatcttt acggaatgct ggccggtggt gctgctgtat
     4081 atggctttaa ccggggattt attcgcccgg cagcggagat ggaaacctat atgattcgcc
     4141 tgaactccct ttacaaaggg gatcgggcga aaactgatga tgtccgcagg tgggcaatac
     4201 agaatgcgaa ggaaaccacc tgggggctgg ctggcgtcat gcaggaatat acgtccagtc
     4261 tgggctttgg tatgagtgac agggaggccc gaaattttgt caccatgctt caggatcagg
     4321 gtgccgtggg cggctggtca ctgtccgatg cacagggggc atccctgcaa ctgaaacaga
     4381 tgtatgcaag aggcagtatt caggcggcgg atgcaaatat tcttgccacc tacggcatta
     4441 atgcctaccg cgtgctggct gatcgtctgg gtgtggacca gaaagtggtc agaaaacttg
     4501 gtgaaaaagg gctgcttggg ccggacagta ttcgcctgtt atttcagacg ctggcggaac
     4561 aggccagggg agcacaaaag gacgcaatga attcctggag tggcctgacg gccatgatgg
     4621 gtgacgtctg ggaccagttt gcccgggatg tgatggacag tgggccattt gaaaaactga
     4681 agggtaatct caggggattt ctttcatggg ttgacagtgc caaatctgat ggcagttacc
     4741 attccctggc ggaaagcacg gcatcagcca tgaaccaggg ctttgagtat gcccgtgatg
     4801 cagtgactgg gttttatcag gcgatcagaa aggtacgcga cacgcttcag gctctgcggg
     4861 atgcgggcta tggtgatgcg ctggatcgca ttggtcaggg ggcacagacc gccgcgaaat
     4921 acctgctgta catgtatctc gccacccgtg ccctgaaaat ggcgcgggct gtcggaacgg
     4981 ggattatacg tccgggtgct gcattgctgg gatacggatt atcagcggcg gccttcctga
     5041 cctcaccttt ccggcgctct cctccggccg gaacaccacc aggcacatct cagggacgag
     5101 ggcaacgttt tataaacttt cttaccgggg taaatccggc agccgttcag ccagtacttg
     5161 taacaaactg gcccacagga ggactgtccg gcaccggcgc gaccgataca tcctcaggcg
     5221 gacgtcaggg gcggggccgc aggaaacgcg gaccggggcg cggaagaccg gtcacgcctc
     5281 ccctgccacc gtccccagtg ccaccgtctc cctcttctgg tggggcaggg ttctgggggc
     5341 gcatgatggg gcgcgccggt gggttacttt cgtctgtcgg tagccgcatg gggctggggc
     5401 gtttctccgg ctttttccgc agtgccggtg gactggccgg ccggctgggt ggaggtgccc
     5461 tgtgggctgg cgcgatggca gccccggttc tgctggatgg cagcgccagt gccacagaca
     5521 agggagaagc ggtcggctct cttgcaggca gtattgccgg tggtgcgctg ggggcggcag
     5581 ccggacctgt ggggattgcc attggttcta cggtgggcag ctatctcggt aactatctgg
     5641 ggggatggct gactgaagcc tggcagaaat tacggggcag tgatgatgaa agcagtggac
     5701 aggcggtaca aaaagcctct gcccgtgtgg agctggttgc cccggaaggg tggcaggctc
     5761 gcagtattga tattgatgac acgtccggtc acggactgga tgtgaatgtc tggaacggag
     5821 ggaattatgg cctctggtga cggtcgtggt gcgttccgtg gtgtgccgtt cctcgtctgg
     5881 cgtgagcagc gcgaacgtgg cgggcgaaac attgtccgtc gggaatatcc gttacgggaa
     5941 accggtggtg cagacgatct gggacctaaa ctggctgaat ttacgttcag tgtacttgtg
     6001 atgggggatg atgttcagac acagcgaaat cgtctgcgtg atgcccttcg tgctcccggt
     6061 gccggggagc tgcttcaccc ggattacggc acgttaaatg tgctgataaa cagctttgaa
     6121 agtcgttata acgctgctga acagggcgtg gttgagttca ccattaatgt cacgccggta
     6181 agtgatgata ccgcacctgc tgtcacacag gacacggccg ccattctgga gcagaagagt
     6241 accaccgcgc tcgggaaggt ttttgaaacc ctggaagcgg gctggacggt gatttctgac
     6301 ggaatgcacg atgtccaggc aatgaccgaa accatcagcg ataaggtgtc cgccctggaa
     6361 aatgccgttt ccggcatggg gattgtacag gatatcagtg cctttactgc cacgttcacg
     6421 gcgctgaaag ggaatgccac ggcattactg acagcacctt cccgcatggc ctcatctttt
     6481 gcggggcttt tcagtgccct gatcaccctg ccatcgctgc cgtcactgtc ttcaggaggc
     6541 ctgaataccc ggcccggtgg cagtatcggc cggacgacat ccgccttctc tcagggaatg
     6601 ccgcaactgt acaggacatt atcttccctg cgttacgtgc tggatgagca ggataatccg
     6661 cagactctca tcggtctgac accggcagca caaaagaata ttcgtctgat acgggcggta
     6721 atgcagagtg ctgccgtggt ggcccaggca cagaccgtgg gaaaactgct ggatcaggtt
     6781 ctcagtcagg aaaccctgcc ggacagtgac gcagcccacc gtacctggcc tgtctggctg
     6841 gaaagttcag ttgatcttca gcgcattaac cgtgacttaa gcgaagcgct cgaacggcag
     6901 gtgatgacac tgtccgggca ggggtatacc gccacggcgc tgacgcttcg tgatgccaga
     6961 ctggcactga cagaggatct gaatacacgg ggagttcagc tacccggtgc aacagtagtg
     7021 actgtacgta ccaccgagcc tgcactggtg accctgtacc gtgccaccgg gaacagtacc
     7081 ggctggcaac gttttgtgcg ccgtaacggt attgttgatc cgctgttcat tcccggaggc
     7141 cattcagtgg aggtgattag tgagcagcag ggttgaactg tatctgggcg gtgagatttt
     7201 ttccggctgg ctgacggtga gtgttcgtcg ctctcttgaa catctggcgg gctcctttga
     7261 actgggggta atgatgcccg gtgtacgcct tccgtcatcc gtccgtgccg gtcagtctct
     7321 ggaattgcgc attgacggtc agcctgtgat cactggctgg ctggatcagg tccggcagcg
     7381 catcagcgcc acgcgttttc agatcacgct cagcggacgg gataaaaccg gtgacctggt
     7441 ggactgttca gccattcatc cgggcagcca gtggaggaac cgcacgctgg agcacattgc
     7501 ttcagatttg tgtgctccgt tcggggtcac ggtgcgctgg caggtaaatg atgcaacggc
     7561 agcccggccc ttttccacct tcacactgga aaactcagaa accgtggcag atgcgctgac
     7621 acgggccgcg agacacagag gcgtcctggt aaccagtaat gccgccggtg aactggtttt
     7681 cactcaggcc ggcagtcagc gtggcgacac gctgacgctg ggcgaaaatc tgctggattt
     7741 ggatcacaac gtggatcacc gtctgcgcca cagtgaatac cgtgtacggg ggcacgggcg
     7801 tggtggtggt catgccgggg atgcactgac agccggaacg ctggccgcac ccgttggtac
     7861 ggtgacagac agtgccatcc accgttacag accgaaaatt gtgctggcgg atcatgctgt
     7921 tgatgcagac ggtgcacgcc agagggctgt ccgggaaatg cgccgggcgg ttgcccgctc
     7981 tgtgcgcctg acagccaccg tgcggcactg gtttcgggag aacggccagt tgtgggatat
     8041 caacctgctg acggctgtca cggctccccg caccggagtg gaagagcgtg atcttcttgt
     8101 ctgtcaggtg gagttttcgc tggatgcaaa tcacggcgaa accacccgtc tgattctggc
     8161 accccgtgac ggctttattg ttccggcaga gccgggaaac agcggaagcg gaaatgcggg
     8221 tgacgtggac gccttcgtgc gggcacagat gaaaaaacag gggattaaat tcaatgatga
     8281 atgacgaagt catcagccgc cttctggccc ccgtgatgcg gggtgttcgt ctgctgttcg
     8341 ggcgtggtgt actgaccggc acaacggaca cgctgaaaat ccagaatgtg caaatcaccg
     8401 gtatggatgg tgaaaccttt gatgacgttg aacgccccca gcagtacggg caaatcagcg
     8461 tccccctgcc tggtgcggaa gtttttctgg cctgtgctgg cggacaacgg gatcaggccg
     8521 tggtgcttgt ggtggaagac cgccgcagtc gcccgaccgg acttaccgcc ggagataccg
     8581 gcgtgtatca ccatgagggg caccgtatac gtctgacaaa gaacggccgg atcattgtga
     8641 cctgtaagac gctggagatt tacgccgatg aaggtgttca ggtggataca ccggaggctc
     8701 actttaccgg taatgtcaca gtggataaga acctgcatgt caaaggcaat gtgtccattg
     8761 acggcaccgg aagatcacag gggacgttca cgatgtccga agcggttatt gccgggatca
     8821 cctattcagg tcatgtgcat cacgataacg gtgaaggcag caaaacggga ggaccagaga
     8881 atggctgata ttgctgtcgt ctgggatcag ggttgcggtt ccctgcaact gaacggcgca
     8941 gatcttctga cggataacag cctgctgact gcggtcatta tttcactgtt tacggacagg
     9001 cgggcgctgg attccgatga aatccctgac ggcacccgtg accgtcgggg atggtgggga
     9061 gacagtttcc gggagcgccc cgttggctcc cgtctctggc tgttaagccg tgaaaagacg
     9121 ctgtcctccg tggtcagccg tgcacaggcc tatgctgatg aagcgctggc gtggctgcat
     9181 aaaagcggtg ctgccacatc cgtggtatgt catgccatgc gtgtggggca tgctcgcctt
     9241 tcgctttccg tgaaaatcac cctgccggac ggaagcagac atccgatgat tttttatgct
     9301 gatatgaagg gggaatgatg ccttatcagc ccttaccact ggcgcaactg atcacacaga
     9361 cacaacagga tatcagccag cgcctgcccg gttcgcagcc gggcgtgaat gaaaccaccc
     9421 tgaatgccat tgcgtatgca ctggcgggtc tgtcagcaca ggagcatgaa catctggcct
     9481 ggatctcccg gcagataatt ccgacagaag ctgatgaagc cgaactcctg aaacactgcg
     9541 cattctgggg tgtcatccgt aaaccggctt cccgcgctga cggaccggta caactgatgc
     9601 tgaccacgga tgcagggatc acggaaggcg tactccttca gcgaagcgat ggggttgtgt
     9661 accgcatcac cggctctgcg accggaaaag ccggaacact gaatgttaat gtggaggcgg
     9721 aaagtgcggg gcgcgctgga aataccccga ccggaacccg cctgtccttt atcacgccac
     9781 aggcgggcat caaccagaca gccacggtca ccggcacggg actcaccggt ggtgcggatg
     9841 tggaaacggt gccggagttg ctgtccaggc tggtattccg ggtacagaac ccgccatcag
     9901 ggggaacaca gtatgatttt gaacgctggg cacgggaagt accgggcgtg acgcgggcat
     9961 ggtgtaagcc tgaatggcct gaggcgggta gtgttggtgt gacttttgtt caggataata
    10021 accctgacat tttccccgga gaaggtgatg tgaagcgggt ggcggattat atccgcagtc
    10081 atgatgatcc ggcgacgggc cagcccgttg gtcagccact tgggccgaca atcagcgtgt
    10141 ttaagctgac caataagccg gtggcgtttg agatcaggat tgtacccaaa acgccggaaa
    10201 atcaggctgc cgtaaaacag gcattaacag acctgcttta taacgaatcg cggccgggtg
    10261 gacttgtatt gccttcatca ttctggcggg ctgttgcagg ggtgaaagga ctggaggatt
    10321 ttgaagttcg cagcccgctg aagtccgtga tggccggaga tacagagttg ctgaccgtgg
    10381 gggaaatcac atggctgtaa ccctgacccc gcatcagcgc gccctgttgc agttgctgcc
    10441 tgacgggctg gcatgggata agcggccgtc atccgttctt gcggctttgt gcctgggcct
    10501 cagtcattcc acggagcgtg tttcctggac cggtaaccag atgctggcag aacgttttcc
    10561 tgattcatcc cgtctgctgc tggaagactg ggagcgttat ctggggttac cggaatgtga
    10621 tatgaccggc gcaaccattc aggagcgtca gcgttatgcc gggaataaat accggatgaa
    10681 accctctctt aaccgtgaat tttatatccg gtttgcggca gagtttggtt atgaaataga
    10741 tattcagcca tcaccggatt cacagtgggt cagtattgtc acgattaaca gtgaaaccgg
    10801 ctaccggaat atgaatgtgc tggatgatat tctcacgccg ctgcgtattt atgaaggcgg
    10861 tgcgctggaa tgtattctga accgttataa gcctgcatgg cagacgttta tttacgtata
    10921 tgcaaacagt cacgaagagg agaatattta atgttccatg ttgataataa ttccggcgtg
    10981 gcgaatatgc ctgcgctggc accggcgcag agtaatacca ccacctggtt taccgaaggt
    11041 gacggacaaa aaggtatcag ctggattggt caggactggc tgaatattct ccaggccgaa
    11101 ctgctgaata ttctggctga agccagtatt cagccggata aggcgcagtt aaaccagctt
    11161 acgctgtcca ttaaagccat tatcgctgcg aatgcctttt cccggaaaaa caacctgaaa
    11221 gaaattgctg atgccggtgc ggaagcccag cgtcttgccc gtggttatct tggtcttggg
    11281 gcgcttgcca caaaaaacag tcttggtccc ggtgacgtta atgctctggc gaaggatcag
    11341 aaccttgccg acctggagaa tgcgggaacc gcccgtaata atctggatgt ttacagcaaa
    11401 agcgaaggtg ataaccgtta cctgcgcagg gagcagaacg gcgcagacat tccggataaa
    11461 ggcgctttta tcgataacgt cggtttacgg gaaacggtaa ataaggctgc tgatgccctg
    11521 ccatcgggtg gaaccgccgt ggcagcaaac agactcgcca ccccaagaaa tatcaatggc
    11581 gttccttttg acggaacgca ggatatcaac atcacgtcag gaatgacgca gtcaaccgca
    11641 gatggccggt atgtacagaa tgttcagctt ggagcacaga gctatcattc acccggaggc
    11701 aatgaaatat catggaatta cagcgcacct tccggttgta tgctttccgg tattaacgtg
    11761 caggaaaccg gctcccggtc tgcggacaat atcggtgggg tctattatcg cccggttcag
    11821 atttatatta ataatgcctg gagaacggta agttcagtgt aaaccacaga aaagggtgct
    11881 gaatgcaccc tctgaattat tccggttttt ccggccaggg aatcgcctgg taatgcccct
    11941 cactggcaat atcattaaag tgcatttctt taagctgtcg gatatacgcc atccagcgcg
    12001 tcagctgtaa tttattttca tcgctaatca tgtccagttt taattccgtt ttccagtcat
    12061 ccacagtttc gttggcttcc tggagcagcg atgagcggcg cttttcagca accgtcttcc
    12121 agtttaccgg cacctttgat atcaggccat cagaaaactg ccagttaccg tcaatattca
    12181 caccttccgg cagttcgtcc acttcaacaa cagtaaaacc tgccggatat aaagcagaga
    12241 catcttcagc aacagagcag ataatacccg ttacaggcga aatacagagt ttgtatttct
    12301 ttgtgaaaag cgggatagat tcataaaaat cccttccgtc ctcactctgg aaatactgaa
    12361 catcctcacc atagggtttg tgttccgggt aatatcgttt tacgttaata agctccatta
    12421 tttcaccttg tttttgttga aaagacaaaa tcatcatata tttattcaga taatctgttt
    12481 tttgaactac tgcactttta tgcgctggaa gctgttttcc acgaaccatc tatcagaaac
    12541 tgaacaggtc gataatatgc ggttaccgtt gcattaccat caccagcgac atcaagccct
    12601 gtaagtacgc atccgtcagg aacctcaaaa atattatatt gcattccaat cgttgacctt
    12661 gaacctcttc gaatgctttg aacataacgt gagtcagcca cagactgcgt catccccgat
    12721 gtaatattta tatcctgcgt tccgtcaaat gggacaccat taatatttct tggagtggcg
    12781 agtttattcg ctgcaacagc cgctccgttc gatggcaggg cattcgcggc cctgtttaca
    12841 gtatcccgta aaccgaggtt tcagggatgc cgtatatctg ctttaaaagg cctttccacc
    12901 tctttttaag gaaggtaaat acatgctgat tggctatata cgcgtatcaa caaatgacca
    12961 gaacacaggt ttacaaagaa atgcgctgga atgtgcagga tgtgagcaga tttttgaaga
    13021 taaaatgagt ggtaaaacga cacagcggcc gggactgaag cgggcactga agaaactaaa
    13081 acccggtgac acactgatgg tctggaaact ggacaggctg gggcgcagta tgcgccacct
    13141 tgttgcgctg acagatgaat tacagcaaca ggggataaat tttcgtagtc ttactgacaa
    13201 cattgatacg tcaaccccca tggggcgctt cttttttcac ataatgggtg cgctggcaga
    13261 aatggagcgt gaattaattg tcgaacggac acgggctggt cttgcagctg cccgtgaaag
    13321 gggacgaacc ggcggcagac cccgtcgtct cacgcaggaa caatacgagc aggtcgggcg
    13381 tttactggcc gggggtgtca gtcgaagaca ggtggcactt atttttgata tcggcctttc
    13441 aaccctttat cgttattatc ctgcgagcgt ttcggaaaaa taagacgctc cctttgttgg
    13501 gcctttactg aattaacgca aaatggcgtt tatcggtctg tggatatgat aattcagttt
    13561 attcaggaac aggctgatta ttatgacgtc caaatgggtt caactatcat caatgcccgg
    13621 aaactttact gttaaagttt ccggtggtac agcggcattt cttgaggctc cctttcctcc
    13681 ggccgaaaca aaaggaggaa tgacatttgc tgactgtctc atcagtttta atacacggga
    13741 ttgcctgtgg gtaaggccag tatccggtga tccgagtgtg gaaattaccg gagcgggtat
    13801 tggtgcggtc attccgttaa gtgctgatgt tgccggtact gctgaaccgt cagactggga
    13861 taatgctgaa acacataccc gtccgtcagg aaatgaaact gcttccagct ccccttcctg
    13921 gtattacgtg gtggttcttg ctggtcagtc aaacggtatg gcttacggtg aaggtctgcc
    13981 actgccggag acatatgacc gccccgaacc gcgtattatg cagttagccc gtcgcagtac
    14041 agtcacgccg ggaggtaaag cctgtcagta taacgacatc attctggccg accactgtct
    14101 gcatgatgtg caggatatga gtggaaaaaa tcacccgaaa gcggatgtgg ctaaagggca
    14161 atatggcacc gtgggtcagg ggcttcacat tgccaaaaaa ctgctgccgt ttattccggc
    14221 agatgcgggg attctgctgg ttccctgctg ccgtggtggt tctgcattca cggcgggtgc
    14281 tgacggcacg tacagtgaca gcacgggtgc ttcagaggac tcagcccgct ggggtgtgga
    14341 taagccgctg tataaggact taatcagccg gacaaaagcg gcactggcga agaacccgaa
    14401 aaaccgcctg cttgctgtgg tgtggatgca gggcgagttt gatattgatg cgaagccgac
    14461 ggagcattcc gcgctgtttc tggcgatggt ggaaaaattc cgcgcagacc tggctgaaca
    14521 ggcggaacag tgtaccggtg gcagtgctgc tggcgttccg tggatttgtg gcgataccac
    14581 gtatttctgg aaacagaaaa atgaaccggc ataccaggca atctatggcg gctacaaaaa
    14641 caaaacagat aaaaacattc atttcgttcc attaatgaca gatgaaaatg acgcgaatgt
    14701 acccaccaac aatccggcag aagacccgga tattgaatcc attggttatt acggctcaac
    14761 gtggcgtaac agtgccgcca cctggacatc tgcggaccgg gccagccatt tcagttcatg
    14821 ggcacgtcgt gggattattt ccgaccgtct ggccacggca gttctgactc atgcggggcg
    14881 aaccacagta aaagccgatg ttccatcttc tgaaactgaa gcaccagtgc cgtcaccttc
    14941 agaaactgaa gcagtaacca caacactgct gtcttatcgt gtcagtgagt cagaaggaaa
    15001 tctgaaagcc cagggctggg agccggctgg cggtaaagct gaaattatca gtgatgcagg
    15061 aggcacaggt ggtaaggcaa tgaaactgac caaggaaaca ggtaaatcat cttggtatct
    15121 ggatcatgat gccggtactg gtgctgaact gctgaaaaat ggtggtttaa tcagttgtcg
    15181 ttttaaagta cctggcgacc tggtggcaaa ccagtatgtc atggggcttt actggccggt
    15241 ttcctctctg ccgcagggtg tcaccctgac aggggatgca gggaataatc tgctggcatc
    15301 gttctacatc cagacagacg caaaagacct gaatgtgatg taccacaatg caaaagtggc
    15361 aacaaataac cagaaactgg gaacctttgg tgcatttgat aatgaatggc atacgctggc
    15421 cttccgtttt gcaggaaata acagccttca ggttattccg gttattgatg gtcaggatgg
    15481 tgccgcgttt accctgacgc agtcaccagt aggaactttc ccggtggaca aactccgcgt
    15541 aacggatatc acaaaaaatg caacttaccc ggtgctgatt gacagcattg tggtggaagt
    15601 gaaaaaagcg gtaacagaat aaaaaaatcc cgccggtcat gatatggctg gcgggagcta
    15661 cctaacccat aaaggaaaat aaaaatactc ccgaaggagt ggttatcagt cgcttcgatg
    15721 tttttacccg ataacgaatt atgtagtcaa tatcaaaggt aaggttatat gacattcgtt
    15781 catacaatgc tgctttattt ctgcgctgtg gtcagtgcgt tatatctggt gagcggtggt
    15841 tataaggtca tcagaaatta tattcgccgt aaaattgatg atgcggcagc tgaaaaactc
    15901 agtaaaacag cacaggcacc gtcatcccca aacgacccga ccccgctctg ataacggggc
    15961 aatatacctg caaaaggaga atattcatgc cagagattaa aggcacggtt actgaagagc
    16021 ttgtcaaaca ggcactttat tctgaagaag tgaaccgcgt gctgaaggcg caggttcgca
    16081 aggattttga ggcacaaatc gacgcatatg ttgatgaagt gctggcccga atggttggcc
    16141 gttctccggc tgaaaacagc acggaaaatg atcctcaacc cgtagagcaa ccagagccgg
    16201 ttcagcccgg aactgacggt actatgatgt aacaataccg accggcaggt ttccctgccg
    16261 ggtgagtttg ttaatgcacg ttttgtgtat tttccctcct gggagaacag gcaggacata
    16321 tcagcatcct gaaacaggaa gcatttttgg gtttgtatgt cttgccattt tgagatcgag
    16381 aacggcgata gatgagctgt aagggagtct tacaggcagg gcaaacaggc tctttcatcc
    16441 atgataaaga tctccttttt atgcatctct gctttacagg agaccgggga atatcatagg
    16501 atacgccacg agctcgaagt aaagcattga tgcaagtttt tacagattga cccagcatag
    16561 ttccagctat gctgggtttc acttttatgt agtatcctgt tgatttgaaa catactacat
    16621 atacgcgtga tcctaccttc actgtataaa aagtcaataa aatgatcatt ttatgatcgt
    16681 tttcaccacc taactcttcg ttttctttaa gtaaataatc acctccctga atcattgttg
    16741 atgacttttc aatctatttg ttgtatcaat taaacaaatc gaatcgatcg tttttgtcga
    16801 tcgattgcat gagaaaggca tcaacaacaa agcaggaggt tatgcggtca tgtatcgaaa
    16861 catacggtgc cgacactgca ataagctact ggcacgggcc agtttcagtt atctggaagt
    16921 aaaatgtccg cgctgtaaaa ccctcaatca aatcacatct ccgagcgcca cagagcaccc
    16981 cacatacaca aggaaatcct gtcgtgggga acagaaagca agtcacatcc cgaatcatca
    17041 gcacgcctga acttattcgc tataacgaca atatcgttgg ttatggctcc cgtgagttgc
    17101 gggttgagac aataagttgc tggctggcca gactggtcat cgtcaataaa cattacagcc
    17161 accgtttcgt aaataactcc taccttcatc tgggcatatt ttcagaacgg gaacttgttg
    17221 gcgtaatgca atggggttac gcccttaacc caaacagtgg tgcacgcgtc gtaacgggta
    17281 cgcagaaccg agaatatatg gagcttaacc ggctatggct gcatgactgt atgccgagaa
    17341 actccgaatc aagggcaatc agttacgcgc tcaagctaat caggcagctt tatccgcagg
    17401 tacagtgggt tcagtcgttt gcagatgagc gttgtggctg tcttggtgtc gtgtatcagg
    17461 caagcaattt tgattatgta ggcagccatg aatcaatatt ctatgaactt gatggcgagt
    17521 ggtatcacga gatttgcagg aatgccatta aacgaggcgg acaacgaggt gaacatctga
    17581 gggctaatat tgacagagcc agtgttcata agttccgaca gtttcgttac atccggtttc
    17641 tgaacaagag ggccagaaaa cgcctcaaca caaagctatt caaagtccag ccttacccaa
    17701 aaccacaaac agttaaaact ggtttgaaag agagtgaatg agcgcttaaa aactcattct
    17761 aaaactcaaa agaaaagcgc cgatttagta tcaaataagt tgcgtatcgg cgtttttcag
    17821 cggtatcaaa tgaaatgcaa atcggtatca aaaaaatcgc cgcgctatat gctgcctgaa
    17881 tttggcggtg actcttatac caatgcagac aactttatga ctggtcgagc caatggcgtc
    17941 gcgacttatc gtaatactga tttcttcggt ctggtaaatg gtctgaactt cgcggtgcag
    18001 tatcaaggta acaacgaagg tgccagtaat ggtcaggaag gcaccaacaa cggacgtgat
    18061 gttcgccatg aaaacggtga cggctggggt ctttccacaa catatgattt aggcatgggc
    18121 tttagcgctg gtgcggcata cacctcttct gaccgcacca atgatcaggt taaccatact
    18181 gcggcgggtg gtgataaagc agacgcgtgg actgctgggc taaaatacga tgctaacaat
    18241 atttacctgg caaccatgta ttcagaaacg cgtaatatga ccccgtttgg cgacagcgat
    18301 tatgctgttg caaacaaaac ccagaacttt gaagtcactg cacagtacca gtttgatttt
    18361 ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgacctgca cgctgccggt
    18421 ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc cgatgttggc
    18481 gcgacttact atttcaataa aaacatgtcc acttatgttg actataaaat caacctgttg
    18541 gatgaagatg acagtttcta cgctgccaat ggcatctcta ccgatgatat tgtcgcttta
    18601 ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt tttctgctta
    18661 ttcttcctcc tttgatctaa attaaaaatg tgaactccgt cattacacaa aaagtgtcat
    18721 ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga acgattcttg
    18781 tccctatcga tatttccgat tcagaattaa ctcaacgcgt gattagccac gttgaggcag
    18841 aggcaaagat tgatgatgca gaggttcatt tcctgacggt aataccttca ctgccctact
    18901 atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac ctgaaagcgg
    18961 aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc gacagagtgc
    19021 atgtccatgt tgaggaaggc tcgcccaaag accgcattct ggaaatggcg aagaagatcc
    19081 ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat ctgctcggtt
    19141 ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg cgctgacact
    19201 aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg acattttccc
    19261 ctctaatccg taccatacac gccactgttt ttatatcaga tttcttatgc tgggcattcc
    19321 ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta aatgtcgcaa
    19381 aatcaagaaa ttagtaagaa agaacaatac aacctgaaca aattacaaaa acgtctgcgt
    19441 cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga tcgcatcatg
    19501 gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg caatttgcag
    19561 caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc
    19621 ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta caagattgtt
    19681 gaagagaata cttacggtat cgtgaaagag aagattccag agggcaaaac cacttgctca
    19741 ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg caacggaact gggggcgacg
    19801 aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc
    19861 tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg caaacatatc
    19921 gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa
    19981 gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca acgtcaggtg
    20041 attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga gacgatgttc
    20101 agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt cgattttaaa
    20161 ggcattaccc acggttctga agtggttaac gggggtgatc tggcgtttga tcgcgaagag
    20221 atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt ggatgagtta
    20281 cgactgaatg tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg
    20341 ctactcttat ggaggccgga taagacgcgg ccagcgtcgc atccggcaat cccgaataag
    20401 atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg cacccggcac
    20461 gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc aacagcctga
    20521 cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc tgcgccatca
    20581 agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt ggcttttttc
    20641 ccgccatcga tacacaacgt tgccatttct gcttccagcg gcacaatgga actattagct
    20701 ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt ggcccgctgt
    20761 gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg agctgtacga
    20821 ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac caccagctca
    20881 agcgatttaa tatccagacc acgcgcagca acatcagtag caaccagtac acgggcgcta
    20941 ccgttagcaa aacgtaccag agtctgatcg cgatcgcgtt gctccagatc gccgtgtaac
    21001 gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca atcttttttg
    21061 gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataaccg ttgcaacaga
    21121 ggaattttgc ctttgctgga tgtctcataa aattgttgtt caatgggtgg caaagcatct
    21181 gttgagtcaa tttcaatcgc caaaggatcg cgttgtactc gtccgctgat tgcggcgatg
    21241 gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg
    21301 acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc atccatcacc
    21361 aaagtattca gcgcatccag tgataccgtg cctttttgca agtgatccag caaacgaccc
    21421 ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat accgaacggt
    21481 tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag ccgacgcaat
    21541 tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc ctgggtttga
    21601 aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg
    21661 ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag cgcggcggcc
    21721 tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg ggcgggaggc
    21781 aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg acgcaaagtt
    21841 agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc ggttcttcat
    21901 caggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact ggaacaggat
    21961 cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct
    22021 ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc
    22081 gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa
    22141 gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg caagggagat
    22201 attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc gtttttgtat
    22261 acagacagcc ggaaaaattg cttttgttac aaccatttac tacgatgcaa ccataaagca
    22321 acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa ttgccacccg
    22381 ccgccaggga tcccaccaag gttgacgcca aataaccctg tcagaaaggt actgggtaaa
    22441 aagaccattg ccatcaatga cattgtatag gtacgacgag ctaaattttc ctgcatcacc
    22501 tgagcgattt catccgccat cacgccagtc cgtgctatac aggcgtcgat ttcgtcaaga
    22561 ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc gctcatccac
    22621 ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat atagcgacgc
    22681 atcacgatta attgtttgcg cagcagagcc aggaatccac gcggtggaat ttgctgatca
    22741 aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa ttcgctggaa
    22801 tgatcggtca acgcatcgca cacatccacc agccaccctc cgcaatcggt cggacccgtg
    22861 ccctcttcca gatcgctcac cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa
    22921 acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg gcgttcatcg
    22981 gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg gctgactcgg
    23041 ggccgagtgc tctcgcctgc cagcgcatca cgtacgttat tgggaagcag cggtgttgtc
    23101 gccagccatt gggcgctttc atggtgtaca taattaaggt ggagccagca gggatgcgct
    23161 tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc cagcatccag
    23221 gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac agtgcctcca
    23281 tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca tctcatcacc
    23341 ccattaccct gaaatga
//
LOCUS       DAZFWP020000075        22811 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFWP020000075 DAZFWP020000000
VERSION     DAZFWP020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.075"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..530
                     /gene="ybeT"
                     /locus_tag="ACHK0Z_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             <1..530
                     /gene="ybeT"
                     /locus_tag="ACHK0Z_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4502753.1"
                     /translation="AIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAA
                     TELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYL
                     KGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQ
                     HYKNAERLLAGLPAH"
     gene            complement(540..1967)
                     /gene="djlB"
                     /locus_tag="ACHK0Z_RS13785"
                     /old_locus_tag="pgaptmp_002757"
     CDS             complement(540..1967)
                     /gene="djlB"
                     /locus_tag="ACHK0Z_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU4502754.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1964..2671)
                     /gene="ybeR"
                     /locus_tag="ACHK0Z_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             complement(1964..2671)
                     /gene="ybeR"
                     /locus_tag="ACHK0Z_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4502755.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2835..3812
                     /gene="ybeQ"
                     /locus_tag="ACHK0Z_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             2835..3812
                     /gene="ybeQ"
                     /locus_tag="ACHK0Z_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGU4502756.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3882..4364)
                     /gene="ybeL"
                     /locus_tag="ACHK0Z_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             complement(3882..4364)
                     /gene="ybeL"
                     /locus_tag="ACHK0Z_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU4502757.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFIRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4599..7181
                     /gene="leuS"
                     /locus_tag="ACHK0Z_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     CDS             4599..7181
                     /gene="leuS"
                     /locus_tag="ACHK0Z_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU4502758.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7196..7777
                     /gene="lptE"
                     /locus_tag="ACHK0Z_RS13810"
                     /old_locus_tag="pgaptmp_002762"
     CDS             7196..7777
                     /gene="lptE"
                     /locus_tag="ACHK0Z_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU4502759.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7777..8808
                     /gene="holA"
                     /locus_tag="ACHK0Z_RS13815"
                     /old_locus_tag="pgaptmp_002763"
     CDS             7777..8808
                     /gene="holA"
                     /locus_tag="ACHK0Z_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU4502760.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8810..9451
                     /gene="nadD"
                     /locus_tag="ACHK0Z_RS13820"
                     /old_locus_tag="pgaptmp_002764"
     CDS             8810..9451
                     /gene="nadD"
                     /locus_tag="ACHK0Z_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU4502761.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9475..10086
                     /gene="cobC"
                     /locus_tag="ACHK0Z_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             9475..10086
                     /gene="cobC"
                     /locus_tag="ACHK0Z_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU4502762.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            10346..10663
                     /gene="rsfS"
                     /locus_tag="ACHK0Z_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             10346..10663
                     /gene="rsfS"
                     /locus_tag="ACHK0Z_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU4502763.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10667..11134
                     /gene="rlmH"
                     /locus_tag="ACHK0Z_RS13835"
                     /old_locus_tag="pgaptmp_002767"
     CDS             10667..11134
                     /gene="rlmH"
                     /locus_tag="ACHK0Z_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU4502764.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11165..13066
                     /gene="mrdA"
                     /locus_tag="ACHK0Z_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             11165..13066
                     /gene="mrdA"
                     /locus_tag="ACHK0Z_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU4502765.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVV
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13069..14181
                     /gene="mrdB"
                     /locus_tag="ACHK0Z_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /gene_synonym="rodA"
     CDS             13069..14181
                     /gene="mrdB"
                     /locus_tag="ACHK0Z_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU4502766.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14192..15280
                     /gene="rlpA"
                     /locus_tag="ACHK0Z_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             14192..15280
                     /gene="rlpA"
                     /locus_tag="ACHK0Z_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU4502767.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15420..16631
                     /gene="dacA"
                     /locus_tag="ACHK0Z_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             15420..16631
                     /gene="dacA"
                     /locus_tag="ACHK0Z_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU4502768.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16742..17005
                     /gene="ybeD"
                     /locus_tag="ACHK0Z_RS13860"
                     /old_locus_tag="pgaptmp_002772"
     CDS             16742..17005
                     /gene="ybeD"
                     /locus_tag="ACHK0Z_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU4502769.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17106..17747
                     /gene="lipB"
                     /locus_tag="ACHK0Z_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             17106..17747
                     /gene="lipB"
                     /locus_tag="ACHK0Z_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU4502770.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIVPRLLENILALLNNPDFEYITA"
     gene            18006..18959
                     /gene="ybeF"
                     /locus_tag="ACHK0Z_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             18006..18959
                     /gene="ybeF"
                     /locus_tag="ACHK0Z_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU4502771.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINLTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19168..20133
                     /gene="lipA"
                     /locus_tag="ACHK0Z_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             19168..20133
                     /gene="lipA"
                     /locus_tag="ACHK0Z_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU4502772.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20234..20437)
                     /gene="tatE"
                     /locus_tag="ACHK0Z_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             complement(20234..20437)
                     /gene="tatE"
                     /locus_tag="ACHK0Z_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU4502773.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20566..21354)
                     /locus_tag="ACHK0Z_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     CDS             complement(20566..21354)
                     /locus_tag="ACHK0Z_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU4502774.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGELLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21447..21830
                     /gene="crcB"
                     /locus_tag="ACHK0Z_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             21447..21830
                     /gene="crcB"
                     /locus_tag="ACHK0Z_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4502775.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21885..22094)
                     /gene="cspE"
                     /locus_tag="ACHK0Z_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21885..22094)
                     /gene="cspE"
                     /locus_tag="ACHK0Z_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4502776.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22269..>22811)
                     /gene="pagP"
                     /locus_tag="ACHK0Z_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     CDS             complement(22269..>22811)
                     /gene="pagP"
                     /locus_tag="ACHK0Z_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU4502777.1"
                     /translation="VAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQPEHYDL
                     YIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDSWNKWEP
                     IAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPVTFQMT
                     YIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5834 a         5729 c         5671 g         5577 t
ORIGIN      
        1 tcgcaatatt tatagttgca gcaattacct gtattagcca acccaagaaa acaacgttgc
       61 gtgataaagc catggtgaat tatgcctttg attatttaag ctcaccaggc agtctaccgt
      121 taaccacggc agccacggag ctttccgcga ttcatggtca ctcaacatcg caatatcgcc
      181 ttggcgaatt ttatcttcat ggtagcgacg gtaaaccact ggattataca caggcgagat
      241 actggtatga gcaatcagca gaacaggaaa atccgcgcgc acaaagcaaa ctggggtgga
      301 tctacctgaa aggcctggga gtcaaacccg atacccgtaa agcaattctc tggtataagg
      361 aagcagctga acaagggtat gctcatgctc aatatacttt gggtttgatc tacagaaatg
      421 gcactggtat taatgttaac cattatgaat ctcaaaaatg gttaaaactg gccgccaaac
      481 aacattacaa aaatgcggaa agattacttg ccgggcttcc cgcacattaa cgtcgactgt
      541 caaccgaaca gatggagtat tttccctatc aaaccagcaa agataaaaat atagaaaata
      601 attttaatga tgcccagaca gcctggccca ccatcgtcaa cgggcattgg ctgcggttgc
      661 ggctccggtt caacagtttg cttctcggta aaattcgccg cctgaattaa taatcgacga
      721 ataattaatg cttccagtgg acaacgatct tctgcatcaa ggcggctcac aatcggcatt
      781 aatgattctt cggttggttg gttcgcctgc actaattgtt tggcaactaa atataatggc
      841 cataagggat gttctgtatc acgcgtgagt gagatccgcg gcttacgtcc tttgtgcaaa
      901 accatctcga ccagtgaacg ccccagagca gagagttttt catcgctgag tatttcgtat
      961 aaatgaaaac gcgtacgggc gctgtcgccg gggatataaa gtagcggttt tccctgatcg
     1021 ttaaccagtt cacgagcttc aatcatcatc accactaacg ggtaataatc gacccgatgc
     1081 tgacggcacc attgcaatat taaagcatcc gcctgggtgg agggatactc gcgccagtag
     1141 tcacaaagtt ctgccagcaa gctttcgcct tctccacaat agacgcgttg tgcgtattca
     1201 taataacgcg catcttcatt atcagattca gcttcccgcc acgaccgggc gtaatccagg
     1261 agttcgggaa ttcccaggcg acctgcgcta taccagcgga gtaaatttct gtgcagtttt
     1321 gcatcatcgg ggataatcac tgcgcgatgt tgcgctaaat acaatgtcag ccattctgga
     1381 tgcgatgacc aaatccgagc gaacatttgg taaaaatcga tggtgtcatt ttgcacggca
     1441 acgggcagat gcagaatggt ctggaagtta aaaacattcc ccttcgcaat ggcatataaa
     1501 aaggattcca gttcttcttc gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat
     1561 ctttctgcta ataacaccac gcactcgaag gataaatggg cagtgttcat ggcgattgtg
     1621 cacagcgacc agcgtaattc atcaatctct tccatcgagc aataatttaa ttgctgaatg
     1681 aatcgctgcc aggcggaggg cagaaaacgt tcgctatcag aggcaagtaa cgcacgaaag
     1741 gcgagcagaa tttcatgttc tgctacctca tattcttctg gttgccaaac agatttagca
     1801 ggcgactgcg caatccgtag cgcctcctca tacgcctgac gaagttgttt aaaaccctgc
     1861 ggatcggttt ctggatgaaa ggacggtaac agcgccagat aagcgcggcg gataatatcg
     1921 acgtcagtcg tttcctctat ttcgagaatc ttccagcaat ttttcatagg tcgctcatat
     1981 ccttaagcgt ttccgggcat gcagaatagt aagccaatgg ttgccaggac aaggtagggt
     2041 aatacgcatc atcactggca aataattgat aaaacatatc attgccggaa tcgtcgtatt
     2101 ctttacgcgc taagaattcg cgtaataact gcagatgatc atcttcggga gactgccaat
     2161 attgccgacc aaaggtataa ccggcaaagt attgccgcca gttacgataa taacgtagag
     2221 cccgtagatg aatgcgcgac tggatccata gactttcttc ttcgctcaac cagttattaa
     2281 gtacgcccat tcggcttaaa aaacccatcc ggacataatc ccaggcttta attcctccct
     2341 caccacagca ttcggcggtg ctggcgacaa attgcgcata agcctgacct tgctcattta
     2401 gttcagcaag atagtcacgc cattcacatg gcgaataacg aaaccagcgg tgataaaacc
     2461 ctgccaaccg agcggcatga ccgttatcga tcattcggtg aattgtgcgc agcaggtcat
     2521 cacgggcatc aattccccag gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt
     2581 tgggtgatga atgcgaaggt aaacagcatt cattgcgtat ttccatcgga gtggaaagtg
     2641 caaacaatat tttttgcgac tccatgtcca tataatttat ccttaaatat tcattaaatg
     2701 aatattagcc gcattgcgag gctcgtttat ttatactttt ctgacgcgac ataatacgca
     2761 taaaataagg cttacgcaaa aatggaacac ataaaaatat aatatgtttt atctgcaacc
     2821 gtaaggaaat gataatgatt ttcacgtcaa gttgctgcga taatttatca atagatgaga
     2881 ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata tattgttggg ttttattata
     2941 atcgcgatag cgcaattgat tctccggacg acgaaaaagc cttttactgg ctgaagctgg
     3001 ccgctgagca aggtcattgt gaagcacagt attccttagg gcaaaagtat accgaggata
     3061 aaagccgtca taaagataat gagcaagcca tcttctggct gaaaaaagct gccctacaag
     3121 gccatacttt cgcttccaac gcccttggct ggacactgga tcgtggagaa gcccccaatt
     3181 ataaagaagc ggttgtctgg tatcagatag ccgcggagag cggaatgtct tatgcgcaaa
     3241 ataatcttgg gtggatgtac agaaatggca acggagtcgc aaaagactat gcgctggcat
     3301 ttttctggta caaacaagct gcattacaag gccatagtga cgcgcaaaac aatctggccg
     3361 atctttatga agacggaaaa ggcgttgctc aaaacaagac actcgccgca ttctggtatt
     3421 tgaaaagcgc acagcagggt aatcggcacg cccagtttca aattgcatgg gattataacg
     3481 ctggcgaagg ggtggaccag gactataagc aagcgatgta ctggtatctg aaggctgccg
     3541 ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat gtataaacac ggacaaggcg
     3601 ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg
     3661 ccactgcctg gtataacctg gccattatgt atcactacgg agaaggaaga cctgtcgatc
     3721 tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc
     3781 aggaaattcg tgagactgaa gatttactgt agagataaaa acaagggccg gggaatttca
     3841 ttcggcccat attctcttta ttgctgtgcg aaatcagact attacggctc aaacgggcgt
     3901 ctctggaact ggtcatgacc acatttcggg catagcgtca gcacttccgg tgtgtagatc
     3961 gggagatgga agtgacattt ctcgcagacc agatttccca gcccgaccac ttctccgctg
     4021 tgataaaccc catgatgatt gaggtcctgg aaaacttcgc gccattcaag ctgcgtttta
     4081 tcggtgatat ctgccagctc ctgccataag ctttctttaa tcacccgtat aaagacgctg
     4141 tcagattctt ctttcaggct ctcttcatag ctcatggcga actcttccag gtcacgtctt
     4201 acagctcgcg tcagctcatc gacctcggtt cgcgttaact cccctgtttt tattacgcgc
     4261 tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc cattgcgcag gcgttcgctc
     4321 agtgacgcga ccagttcacg gtaatattga gcaaccttgt tcatcgtttc atctcccggg
     4381 tcgttaactg actataataa tagacgttct ttgttccttg ctttgctaat acgcccacaa
     4441 actctgtaaa ttaatgtatg aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg
     4501 ctgttttgac gcggacgttt gggctatgct atgcggatct gaaaaaccac ctcaacgcta
     4561 catttgtagc cgtattgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg
     4621 aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt gaagtaaccg
     4681 aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac
     4741 tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgc taccagcgta
     4801 tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc gtttggtctg cctgcggaag
     4861 gcgcggcggt gaaaaacaac accgctccgg caccgtggac gtacgacaac atcgcgtata
     4921 tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcaacct
     4981 gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc
     5041 tggtatataa gaagacttct gcggtcaact ggtgcccgaa cgaccagacc gtactggcga
     5101 acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgaa cgtaaagaga
     5161 tcccgcagtg gtttatcaaa atcactgctt acgctgacga gctgctcaac gatctggata
     5221 aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc ggtcgttccg
     5281 aaggcgtgga gatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta
     5341 cccgcccgga cacctttatg ggttgtacct acctggcggt agctgcgggt catccgctgg
     5401 cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt tattgacgaa tgccgtaaca
     5461 ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat actggcttta
     5521 aagctgttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga
     5581 tggagtacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc gactacgagt
     5641 ttgcctctaa atacggcctg aacatcaaac cagttatcct ggctgctgac ggctctgagc
     5701 cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgaattca
     5761 acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg
     5821 ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttctcgt cagcgttact
     5881 ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg accccggacg
     5941 accagctgcc ggtgatcctg ccggaagatg tggtgatgga cggcattacc agcccgatta
     6001 aagcagatcc ggagtgggca aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg
     6061 acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact tgcccagagt
     6121 acaaagaagg tatgctggat tccgaagcgg ctaactactg gctgccggtg gatatctaca
     6181 ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc cacaaactga
     6241 tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg tgtcagggta
     6301 tggtgctggc agatgccttc tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc
     6361 cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg
     6421 caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta
     6481 tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt
     6541 ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggtgtggaa ggggctaacc
     6601 gcttcctgaa acgtgtctgg aaactggttt acgagcacac agcaaaaggt gatgttgcgg
     6661 cactgaacgt tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa
     6721 cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg
     6781 cgattatgga gctgatgaac aaactggcga aagcaccaac cgatggcgag caggatcgcg
     6841 ctctgatgca ggaagcgctg ctggccgttg tccgtatgct taacccgttc accccgcaca
     6901 tctgcttcac gctgtggcag gaactgaaag gcgaaggcga tatcgacaac gcgccgtggc
     6961 cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg caggttaacg
     7021 gtaaagtccg tgccaaaatc accgttccgg tggacgcaac ggaagaacag gttcgcgaac
     7081 gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttaccgtg cgtaaagtga
     7141 tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg
     7201 atatctggca acattgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca
     7261 tctgcgtgat accacgcagg ttccttccac tatgaaggtc atgatcctgg actcaggcga
     7321 tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt
     7381 gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta aagtgagcat
     7441 cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc agatgatcat
     7501 gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc tacccgatta gtgccaaagt
     7561 cttccgttcg ttctttgata acccgcaaat ggcgttagcg aaagataacg aacaagacat
     7621 gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat
     7681 ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg accacaacgg atactccggc
     7741 aacgcctgca cgcgtctcca ccacgctggg taactgatga ttcggttgta cccggaacaa
     7801 ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct
     7861 ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag ctgcggcaca aggattcgaa
     7921 gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt ttcgttatgt
     7981 caggctatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc agaaaacgga
     8041 ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca tgacgacctg
     8101 ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact
     8161 gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagcttccc
     8221 cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc tgcaaatcag
     8281 gtgctctgct actgttatga aggtaacctg ctggcgctgg ctcaggcact ggagcgttta
     8341 tcgctgctct ggccagacgg caaattgaca ttaccgcgcg ttgaacaggc ggtgaatgat
     8401 gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc
     8461 gcgttgcata ttcttcagca actgcgtctg gaaggcagcg aaccggttat tttgttgcgc
     8521 acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc ccatacgcca
     8581 ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgggcgag
     8641 gcgttaaatc gcttaagtca gacgcagtta cgtcaggccg tgcaactcct gacacgaacg
     8701 gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagctgga agggttatct
     8761 cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt
     8821 acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctta aacccgtgga
     8881 aacgctggcg aatttgattg gtctgacgcg ggtcacaatc atccctaata atgttcctcc
     8941 gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat
     9001 tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg ccccctctta
     9061 cactgcgcaa acactgaaag agtggcggca ggaacaaggg ccggacgtgc cgctggcgtt
     9121 tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact
     9181 cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc
     9241 gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc ttcaccttca
     9301 gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat
     9361 ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta ttgccggaac cggtactgac
     9421 ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga
     9481 ctgtggttaa ttcgtcatgg cgaaacgcaa gcgaatgtcg atggtctgta cagcggtcat
     9541 gcgcccacac ccctgaccgc acgcggtatt gagcaagcgc aaaatctgca tacgctgcta
     9601 catggtgttt cctttgatct ggttttatgc agtgaactgg aacgggcaca gcataccgcg
     9661 cgactggttc tcagtgaccg ccagctcccc gttcatatca tccctgaact caacgaaatg
     9721 ttttttggcg actgggagat gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac
     9781 tatagcgcgt ggtgcaatga ctggcagcat gcaatcccca cgaacggtga aggatttcag
     9841 gcattttcgc aacgtgtgga acgctttatc gcaaggctta gtgaatttca gcactatcag
     9901 aatattttag tcgtcagcca tcagggtgta ctgagtctgt taatcgcccg tttaattggc
     9961 atgcctgccg aggctatgtg gcattttcgc gttgaccagg ggtgttggag caccattgat
    10021 atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaat
    10081 gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacaga cccgggcagg
    10141 ctgatattct ccgcagccag acttttttcg ccagacacga ctttgtagaa attgttttac
    10201 aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat
    10261 tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc
    10321 tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga
    10381 caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcaag gcaaatccag
    10441 catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta tgtccattgc
    10501 tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga
    10561 aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga
    10621 agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact
    10681 tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg
    10741 tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa aacgcggcaa
    10801 gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg
    10861 caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc
    10921 cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga ttggcgggcc
    10981 tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc tgtcgacgct
    11041 taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag
    11101 catcaccacc aaccatcctt atcaccgtga gtgataaggg agctttgagt agaaaacgca
    11161 gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg
    11221 ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct
    11281 gtataatctg caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat
    11341 taagctggtg cctatcgcgc ccagccgcgg cattatctac gaccgtaatg gtatccctct
    11401 ggccctcaac cgcactatct accagataga aatgatgcca gagaaagtcg ataacgtgca
    11461 gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc gatgacgata ttgctgcatt
    11521 ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctgac
    11581 cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa
    11641 aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt
    11701 gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta
    11761 tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag acgtggtgca
    11821 cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt
    11881 aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca
    11941 gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggttgtca ccgatccgcg
    12001 tacaggtggg gtgctggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga
    12061 cggtatctcc agcaaagatt attccgcctt gttgaacgac ccgaatacac cgctggtgaa
    12121 ccgcgccaca cagggggttt atcctcccgc gtctacagtt aaaccctatg tggcggtttc
    12181 ggcattgagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag gctggtggca
    12241 actgccaggt tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa
    12301 tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat
    12361 ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat
    12421 cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt
    12481 taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggtcagg gttactggac
    12541 agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa
    12601 ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc
    12661 gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat
    12721 gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tactttgcca gcgcaccgta
    12781 caaaattgcg gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta
    12841 taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc
    12901 atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggcggcg cgggtccggc
    12961 ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga
    13021 tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaatcat gacggataat
    13081 ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatttta
    13141 ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc
    13201 atgatggagc gcaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg
    13261 caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt
    13321 ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gtgctcaacg ctggctggac
    13381 ctcggtattg ttcgttttca gccgtcggaa attgccaaaa tagccgtacc actgatggtt
    13441 gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga agaacactgg catcgcgctg
    13501 gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc
    13561 ctcgttgcgc tttccggtct gtttgtactg ttcctctctg gccttagctg gcgtctgatt
    13621 ggcgtcgcag tagtgctggt ggcggcgttc attccgattc tgtggttctt cctgatgcat
    13681 gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgcg
    13741 ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc
    13801 tggctgcacg gcactcagtc acagcttgaa tttctccccg aacgccatac cgactttatc
    13861 ttcgcggtac tggcggaaga gctgggatta gtgggcattt tgattctgct cgctctctac
    13921 attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc
    13981 gtcatggctg gtggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg
    14041 gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg
    14101 gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa
    14161 atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat
    14221 cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt
    14281 accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcgg atccgcgttt
    14341 cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat
    14401 cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc
    14461 aggcagtaac ctgacggcct ctggcgaagc tttcgatccg acacagctga cggcggccca
    14521 tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat
    14581 cgtggtgcgc attaatgatc gcggccctta cggcaacgac cgcgttattt cgctttctcg
    14641 cgcggcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat
    14701 tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca
    14761 gacttacgcc ctgccagcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc
    14821 tggcccgcag ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac
    14881 cggcgcgccg gtaaccagta gcggtttcct cggcgctcca acgaccttag cgcctggtgt
    14941 actggaaggc agcgaaccga cgcctgcgcc acagcccgtt gttacagctc cgtcgacaac
    15001 gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa
    15061 ctttatggtg caagtcgggg ccgttagcga tcaggctcgt gcgcaacagt accaacagca
    15121 actgggacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg tctggcggat
    15181 ccagcttggc ccattcgcca gcaaagccga agccagcacc ttgcagcaac gtttgcaaac
    15241 cgaagcccaa ttacagtcat ttattaccac cgcgcagtag cgtaaagcag gcatctgatg
    15301 tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag
    15361 tgcatttgct atagtagggc acttttttta attccatcac ggatgtcgta gttcagacca
    15421 tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca
    15481 cagcctttat ctctgctgca catgccgatg acctgaatat caaaactatg atcccgggtg
    15541 taccgcagat cgatgcggag tcctacatcc tgattgacta taactccggc aaagtgctcg
    15601 ccgaacagaa cgcagatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt
    15661 acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactatcg
    15721 gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca
    15781 aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg caatcgggta
    15841 acgatgcttg tgtcgccatg gccgattttg ccgctggtag ccaggacgct tttgttggct
    15901 tgatgaacag ctacgttaac gcactgggcc tgaaaaatac ccacttccag acggtacatg
    15961 gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc ggccaggcat
    16021 tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg
    16081 gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca
    16141 tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg actgaaggcc
    16201 agatgcgctt gatttctgcg gtgatgggcg gacgtacttt taaaggccgt gaagccgaaa
    16261 gtaaaaaact gctaacctgg ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag
    16321 gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct tcgttagggg
    16381 ttgataaaga cgtgtacctg accattccac gtggtcgcat gaaagatctg aaagccagct
    16441 atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta
    16501 tcaacttcca gcttgatggc aaaacgatcg agcaacgccc gctggttgtg ttgcaagaaa
    16561 tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact
    16621 ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata ctaagcatca
    16681 gtaaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga
    16741 catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt acaaagttat
    16801 ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc
    16861 aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat
    16921 cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat
    16981 cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa
    17041 ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg
    17101 ccgttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa
    17161 tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt gatgaaatct
    17221 ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg gagcacattt
    17281 taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg
    17341 ggccgggaca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc
    17401 gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag
    17461 aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac
    17521 tgggtttacg aattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg
    17581 atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa atggctaaaa
    17641 tatcacaatg gaaacccgaa gcgacgacta ataatattgt tccacgttta ctggaaaata
    17701 ttttagcgct actaaacaat ccggacttcg aatatattac cgcttaattc catacctcaa
    17761 tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac atctccttcg
    17821 cgtttgtata ttttctttat cacttgcaga aatgcggtgc cacgccttgc tccgtaactc
    17881 accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt tccgttccaa
    17941 ttagacataa gattattgta attttttaaa tattattttt ccatgaataa aattggagta
    18001 agtgcgtgga tagtaataat caaattgaac cctgtttaag cagaaagtcg tcagaaggta
    18061 aaccacaaat atttacaacc ttacgaaata ttgatcttaa ccttctgact atttttgaag
    18121 ctgtatatgt acataaaggg atcgttaatg cagcgaaagt gcttaatctg accccctcgg
    18181 caatcagtca gtctattcag aaactgcgcg ttatattccc tgacccattg tttattcgca
    18241 aaggccaggg tgtcactcct accgcatttg cgatgcatct acatgagtat atcagtcagg
    18301 gccttgagtc cattcttggc gcgctggata tcgaaggaag ctatgataag caacgaacga
    18361 taactattgc gaccactccc tcggtcggag ccctggtcct tcctgtcatc tatcgggcga
    18421 ttaaaactca ctatccgcag cttttactgc gcaacccgcc catcagcgac gcggaaaacc
    18481 aactcagtca gtttcaaacc gatctcatca tcgataacat gttttgcacc aatcgtacgg
    18541 tgcaacatca tgttctgttc accgacaata tggtgttaat ttgccgcgag ggaaatccac
    18601 tactctcttt agaagatgac agagagacta tcgacaacgc tgcgcatgta cttctgttac
    18661 cggaagagca aaatttcagc ggtctgcggc agagagttca agaaatgttt ccggaccggc
    18721 aaatcaatct caccagctac aacattttga caatcgctgc actggttgcc aacagtgaca
    18781 tgttagcgat tattcccagc cgtttttata acctgtttag ccgctgctgg ccgctggaaa
    18841 aactgccttt tccgtcctta aatgaggagc aaatagattt ctctatccac tacaacaaat
    18901 tcagcctgcg tgatcccatc ctgcacggag taatagatgt tatccgtaat gcattttgaa
    18961 aaagtattgt tacagccaga tcacagcgca gaacgataaa atggcacaat ccaacaacaa
    19021 ttttacattt tcgcgaccgc tttggctgct ttcaggtccg tttcaatgat atactgccag
    19081 tcgttaattc aaaaatagtt gataattaca acaatctatt gaattgaaac gctttccttc
    19141 gtaattcgca actggaacac gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt
    19201 aaataccgcg atgccgataa gatggccctt atcccggtta aaaacgtggc aacagagcgc
    19261 gaagccctgc tgcgcaagcc ggaatggatg aaaatcaagc ttccagcgga ctctacacgt
    19321 atccagggca tcaaagccgc aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc
    19381 tcctgcccta acctggcgga atgcttcaac cacggcacag caacgtttat gatcctcggc
    19441 gctatttgta cccgccgttg tccgttctgt gatgttgccc acggtcgccc ggtagctcct
    19501 gatgccaatg aaccagtgaa actggcgcag accattgccg atatggcgct gcgttatgtg
    19561 gttatcacct ccgttgaccg tgatgacctg cgcgatggcg gtgcccagca ctttgcggat
    19621 tgcattactg ccattcggga aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat
    19681 ttccgtggtc gtatggatcg tgctctggat attctgactg caacgccacc agatgtgttc
    19741 aaccataacc tggaaaacgt accgcgtatt taccgtcagg tacggcctgg tgcagattac
    19801 aactggtcgc tgaagctgct ggaacgcttt aaagaagcgc atccggaaat cccgaccaag
    19861 tctggtctga tggtgggact gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac
    19921 ctgcgccgtc atggtgtgac gatgttaacg ctggggcaat atttgcagcc aagccgccat
    19981 cacctgccgg ttcaacgtta cgttagcccg gatgagttcg acgaaatgaa agccgaagcg
    20041 ctggcgatgg gctttaccca tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc
    20101 gatttgcagg cgaaagggat ggaagttaag taatcctgta ataattactt acatctgtct
    20161 gataaaaaac ccgctttttg aagcgggttt tttgtatcga acaagatatt gagggagcgt
    20221 cctgctcgcc acgtcactct ttatgagaga gcttttcagc ctgaagatca acgtctgcgc
    20281 cttttttcgc cgcagcatcg tcatcattca tcgccttctt gaacccttta atggccgctc
    20341 caaggtctcc gcccagcgta cgtaacttct tagtcccaaa cagcagaacg accagcgccg
    20401 caactactag cagtttggta atactaatct cacccataga taccttcttg acatataacc
    20461 ggcaacagaa cgcgccttaa aaaccagaga atattaacag tcattcgacg cgcgcacaca
    20521 atagcaatct gtaacaaggt gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg
    20581 cggcgcaaag cgacggttgt ttaagacggg cagttgcgcg cgcacctgac gcacacgttc
    20641 gggcgtcact tccgccataa tgagtgcagg catttctgac gctgccgcaa tggtgacgcc
    20701 aaagggatct ataatccggc tttgaccgat atttttgttc ccgcactccc ccgccgccac
    20761 catataacag gtggtatcca gcgcacgagc ggcaagcaac gttgaccaat gatgctcttt
    20821 gagcggcccg cgaacccagg cggcaggaag taccaggatt tcagctccct gtaatgcctg
    20881 tgccagcgcc agctctggaa agcgtaagtc ataacaggtc atcagaccga ccttcatccc
    20941 ctccacctcg agtaacggag cgatttcatt accagcatca acacggcgtg attcctgaat
    21001 ggcaaatgca tcatagagat gcaatttggc ataacgggcg acgatgtttc ctgcctgtag
    21061 cgccaccagc atattccatg cgcgccccgg cgttgaagga acatgaatcg tcagaattgt
    21121 cgtcatcatg ttacgtttac tttctcgccg taaacgtccg aggagttcgc cttccagcag
    21181 ctgtgctgat ttaaccgata gatctgcatc atgatcatcg cgcgccagca atgcttccgg
    21241 caggacgaac agcgatacgt cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc
    21301 agcgttcttt tcccacacag atgtaacagc aaactgtcct gcggcaacca gcattgccac
    21361 ctcctgtcag agtcgtaaaa ttcgccaacg acgattattc ggcgttacac ttatcactca
    21421 tacaaatcaa atagcaggat tttgcagtgt tacaacttct tttagcagtt tttattggcg
    21481 gtggtacggg aagcgtggcg agatggctgt taagtatgcg atttaacccg ctgcatcagg
    21541 cgattccgtt ggggacgctg gcagcaaacc tgattggggc attcatcata ggaatgggat
    21601 tcgcctggtt cagcaggatg acgaacattg atccagtgtg gaaagtatta atcaccaccg
    21661 gattttgtgg cggtctaaca accttctcaa cattttcggc agaagtggtg tttttgttac
    21721 aagagggccg ctttggctgg gcgttactga acgttttcgt caaccttctg gggtcttttg
    21781 ccatgacagc actggctttc tggctgtttt cggcctcaac cgcacactaa agaccaaaaa
    21841 aaaacccgct gattaagcgg gttttgaatt cttgctgacg tatcttacag agcgattacg
    21901 tttgcagcag aagggccttt ggcaccgtta gtgatttcga actctacgcg ctgaccttca
    21961 gcaagagttt taaaaccatt agtctggatt gcagagaagt gtacgaacac gtctttgctg
    22021 ccgtcttccg gagtaatgaa accgaatcct ttggactcat taaaccactt aacgttacct
    22081 ttaatcttag acatcaaaat tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt
    22141 accagtacac caattgtggt acgctttgtc cagtgcaact ttactaaaaa gtgataaaag
    22201 tcgcgttatt tttgatgtta cttattttag ctattgattt taaagaagtt actaaaactt
    22261 catttgtctc aaaactgaaa gcgcatccag gcaaagtaca cattgccatt gttgtaggta
    22321 cccggaatgt aggtcatctg aaaagtcact gggccataac ccacggaggc caatggcagt
    22381 agaaccggga gagggatgta attccagtta tcgcgtgccg ttacgccagc ggtgaatccc
    22441 agacctaaat gaaaattttc atccgccagc ggtcgccagg tactttccca tccgtatccg
    22501 gcaatcggtt cccatttgtt ccacgagtcc ttaaatgcca tggcatacag gccatgccag
    22561 tttccttttt catcccaacg cgacaggcca aaaccgccac cccacggtcg ctcgttatag
    22621 cgatcggttt tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat
    22681 aaatcataat gttcaggctg ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc
    22741 cactcatctg cgttagcaaa aactttacca acgctgatta actgaataaa aacaaaggaa
    22801 aagatagcga c
//
LOCUS       DAZFWP020000076        22403 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFWP020000076 DAZFWP020000000
VERSION     DAZFWP020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.076"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..138
                     /locus_tag="ACHK0Z_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     CDS             <1..138
                     /locus_tag="ACHK0Z_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HGU4502778.1"
                     /translation="YRWHGSAEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTL
                     N"
     gene            212..580
                     /gene="cbeA"
                     /locus_tag="ACHK0Z_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             212..580
                     /gene="cbeA"
                     /locus_tag="ACHK0Z_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGU4502779.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            669..1042
                     /gene="cbtA"
                     /locus_tag="ACHK0Z_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /pseudo
     CDS             669..1042
                     /gene="cbtA"
                     /locus_tag="ACHK0Z_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            1039..1233
                     /gene="yeeW"
                     /locus_tag="ACHK0Z_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             1039..1233
                     /gene="yeeW"
                     /locus_tag="ACHK0Z_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4502780.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1279..1359
                     /locus_tag="ACHK0Z_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /pseudo
     CDS             1279..1359
                     /locus_tag="ACHK0Z_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1648..1794)
                     /locus_tag="ACHK0Z_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /pseudo
     CDS             complement(1648..1794)
                     /locus_tag="ACHK0Z_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1848..2030
                     /locus_tag="ACHK0Z_RS13935"
                     /old_locus_tag="pgaptmp_002787"
     CDS             1848..2030
                     /locus_tag="ACHK0Z_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGU4502781.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(2131..2460)
                     /gene="tmaR"
                     /locus_tag="ACHK0Z_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             complement(2131..2460)
                     /gene="tmaR"
                     /locus_tag="ACHK0Z_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU4502782.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2632..3690)
                     /gene="yeeA"
                     /locus_tag="ACHK0Z_RS13945"
                     /old_locus_tag="pgaptmp_002789"
     CDS             complement(2632..3690)
                     /gene="yeeA"
                     /locus_tag="ACHK0Z_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4502783.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3888..4361)
                     /gene="sbmC"
                     /locus_tag="ACHK0Z_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             complement(3888..4361)
                     /gene="sbmC"
                     /locus_tag="ACHK0Z_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU4502784.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCGTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4480..5646)
                     /gene="dacD"
                     /locus_tag="ACHK0Z_RS13955"
                     /old_locus_tag="pgaptmp_002791"
     CDS             complement(4480..5646)
                     /gene="dacD"
                     /locus_tag="ACHK0Z_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU4502785.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5855..7282
                     /gene="sbcB"
                     /locus_tag="ACHK0Z_RS13960"
                     /old_locus_tag="pgaptmp_002792"
     CDS             5855..7282
                     /gene="sbcB"
                     /locus_tag="ACHK0Z_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU4502786.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(7325..7552)
                     /gene="tsuB"
                     /locus_tag="ACHK0Z_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             complement(7325..7552)
                     /gene="tsuB"
                     /locus_tag="ACHK0Z_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU4502787.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(7566..8624)
                     /gene="tsuA"
                     /locus_tag="ACHK0Z_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             complement(7566..8624)
                     /gene="tsuA"
                     /locus_tag="ACHK0Z_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU4502788.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(8803..10161)
                     /gene="plaP"
                     /locus_tag="ACHK0Z_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             complement(8803..10161)
                     /gene="plaP"
                     /locus_tag="ACHK0Z_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU4502789.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(10151..10213)
                     /gene="yoeI"
                     /locus_tag="ACHK0Z_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             complement(10151..10213)
                     /gene="yoeI"
                     /locus_tag="ACHK0Z_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU4502790.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(10428..11357)
                     /gene="yeeY"
                     /locus_tag="ACHK0Z_RS13985"
                     /old_locus_tag="pgaptmp_002797"
     CDS             complement(10428..11357)
                     /gene="yeeY"
                     /locus_tag="ACHK0Z_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4502791.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(11403..12227)
                     /gene="yeeZ"
                     /locus_tag="ACHK0Z_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             complement(11403..12227)
                     /gene="yeeZ"
                     /locus_tag="ACHK0Z_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4502792.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(12310..12564)
                     /gene="yoeB"
                     /locus_tag="ACHK0Z_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             complement(12310..12564)
                     /gene="yoeB"
                     /locus_tag="ACHK0Z_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGU4502793.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(12561..12812)
                     /gene="yefM"
                     /locus_tag="ACHK0Z_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             complement(12561..12812)
                     /gene="yefM"
                     /locus_tag="ACHK0Z_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGU4502794.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            13095..13145
                     /gene="hisL"
                     /locus_tag="ACHK0Z_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             13095..13145
                     /gene="hisL"
                     /locus_tag="ACHK0Z_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU4502795.1"
                     /translation="MIRVQFKHHHHHHHPD"
     misc_feature    13121..13244
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            13291..14190
                     /gene="hisG"
                     /locus_tag="ACHK0Z_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             13291..14190
                     /gene="hisG"
                     /locus_tag="ACHK0Z_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU4502796.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            14196..15500
                     /gene="hisD"
                     /locus_tag="ACHK0Z_RS14015"
                     /old_locus_tag="pgaptmp_002803"
     CDS             14196..15500
                     /gene="hisD"
                     /locus_tag="ACHK0Z_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU4502797.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNVETFHTAQ
                     KLPPIDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNIGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDTD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            15497..16567
                     /gene="hisC"
                     /locus_tag="ACHK0Z_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             15497..16567
                     /gene="hisC"
                     /locus_tag="ACHK0Z_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU4502798.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDS
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            16567..17634
                     /gene="hisB"
                     /locus_tag="ACHK0Z_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             16567..17634
                     /gene="hisB"
                     /locus_tag="ACHK0Z_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU4502799.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            17634..18224
                     /gene="hisH"
                     /locus_tag="ACHK0Z_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             17634..18224
                     /gene="hisH"
                     /locus_tag="ACHK0Z_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU4502800.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            18224..18961
                     /gene="hisA"
                     /locus_tag="ACHK0Z_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             18224..18961
                     /gene="hisA"
                     /locus_tag="ACHK0Z_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU4502801.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNV"
     gene            18943..19719
                     /gene="hisF"
                     /locus_tag="ACHK0Z_RS14040"
                     /old_locus_tag="pgaptmp_002808"
     CDS             18943..19719
                     /gene="hisF"
                     /locus_tag="ACHK0Z_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU4502802.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            19713..20324
                     /gene="hisIE"
                     /locus_tag="ACHK0Z_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             19713..20324
                     /gene="hisIE"
                     /locus_tag="ACHK0Z_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU4502803.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(20420..21397)
                     /gene="wzzB"
                     /locus_tag="ACHK0Z_RS14050"
                     /old_locus_tag="pgaptmp_002810"
     CDS             complement(20420..21397)
                     /gene="wzzB"
                     /locus_tag="ACHK0Z_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU4502804.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIAQRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YLYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(21544..>22403)
                     /locus_tag="ACHK0Z_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             complement(21544..>22403)
                     /locus_tag="ACHK0Z_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGU4502805.1"
                     /translation="VVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGK
                     ALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLA
                     MRVAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLA
                     NYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGI
                     MKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKV
                     YTRDLFGSD"
BASE COUNT         5765 a         5811 c         5523 g         5304 t
ORIGIN      
        1 taccgctggc acggcagtgc tgaagcgtat accggtcgtg aagtgcagga tattcccggt
       61 gtgctggccg tgtttgctga acgccgtaag gacagttttg gtccgtatgt ccggctgatg
      121 agcgtcaccc tgaactgagt gggaattctg atgagcagaa ttatcgccac taccggacca
      181 ttcttagccg attttctgta aggattttat cgtgtcagac acactccccg ggacaacact
      241 tcccgacgac aatcacgacc gcccctggtg ggggctgccc tgcaccgtga cgccctgttt
      301 cggggcacgt ctggtgcagg agggtaaccg gttgcattac cttgccgacc gcgccggtat
      361 cagaggcctg ttcagcgatg cagatgcgta ccacctggac caggcctttc cgctgctgat
      421 gaaacaactg gaactcatgc tcaccagcgg tgaactgaat ccccgccatc agcataccgt
      481 cacgctgtat gcaaaagggc tgacctgcaa agccgatacc ctcagcagtt gtggttacgt
      541 ttatctggct gtttatccga cgcccgaaat gaaaaattaa ctctccagaa tagccttctg
      601 ctacggcctg gtgttttcac cacgccactt ttccattttt atatctgcat atcaggaaaa
      661 tcttcagtat gaaaacatta cctgtattac ccgggcaggc gaccagttct cacccgtctc
      721 ctgttgaaat ctggcagata ctgctgtccc gactgctgga ccagcactat ggcctcacac
      781 tgaatgacac accttttgcc gatgaacgtg tgattgagca gcatattgag gcaggcattt
      841 cactgtgtga tgcggtgaac tttctcgtgg aaaaatacgc gctggtgcta ccgaccagcc
      901 gggattcagc gcctgtaccc gctctcagtt aataaacagc atcgatatcc tccgggctcg
      961 cagggcgacc ggcctgatga cccgcgacaa ttacagaacg gtaaataaca ttaccctggg
     1021 taagtatccg gaggcgaaat gatgacactg gaagccgaca gcgttaacgt acaggcgctg
     1081 gatatggggc acattgtcgt tgacattgat ggtgttaata tcactgaact gattaataag
     1141 gccgctgaaa aaggttattc actccgcgtg gtggatgacc gtgactctac cgaaacaccg
     1201 gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgatgca gggtatagtg
     1261 ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga agatgtcctt
     1321 accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa caggcttcat
     1381 ttcccctgac gccagcctga atacagctgg cgttttcatt tataaacaga aaggaaaacc
     1441 gataataatg gaatggacgc cccgaccatg aagcaacggt aggatattac tgataaacct
     1501 tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca aaaaatgaat
     1561 tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg gattaaagta
     1621 agcgtcaacg gagcaccgta ttgacgctta tttattggtg agaactacgt tccatggcag
     1681 gagttcgtca acacggttgg agggccattc cggcagtatg ctcagaatat ggcgcagata
     1741 cgcttccgga tcgataccgt tcagacggca ggtgcctgct tttccggcaa caagcaggaa
     1801 tctcagcgct gaagcaggaa ttagaggtaa aaacgcccta ccgggccatg aatcacccgg
     1861 tcattggagt agtaaccaaa gcagatttag ccagcatgga acaaatatct ttggtgaaaa
     1921 gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcagtt aataacaatg
     1981 gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgttaa tttcgattgg
     2041 ttctcagatg agaatgatga cggtgataca cagataagaa tcaatgagca atgagggttg
     2101 ccgggcaacc ctcattgaat aaaacgggaa ttacttcgct tcgccgtttt tcatttcgcc
     2161 catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg catttttgat
     2221 gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga tgccctggat
     2281 tgcttcaacg ctcatccctg gcttgatgta atcgctcagg ttgtccagca acaggacgcg
     2341 cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca gtttgttctt
     2401 acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg tagtttccat
     2461 ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat tcgttagggc
     2521 caacattact catagctgcc gcagatgaca atgcttttat cccttttcac tatcataccc
     2581 tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga attatttgcg
     2641 caaggcccgg caaatcagat tcgatagcaa ctcaagctga tgtgccgttt ccatgttcag
     2701 ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt tattcagcaa
     2761 ctgacgcagc tcttccacag catcgttcaa tttttccgta ttggcaaaaa ccggctgcgg
     2821 attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca tatgctgggt
     2881 atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg cccagtacgc
     2941 attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga taccttcata
     3001 tatcgatttt ggaatacggg tttctttgct ggcgggcgca atcagcccac gcattttcac
     3061 ggcatcggtc aagagttttt gtagatggct ttccagacgt gggcgttcga gtaagttcgg
     3121 tgagaatgca gattgataga cccgattata ctcggtcaga cttttcgcca gttgaatgcg
     3181 ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca gtaaagagcc
     3241 gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg gggaaccaac
     3301 aacaattgcc agcgtcaccc caatcaataa tccttgatac ggtttcttgc ccagcgccag
     3361 ccaaccgcaa agaaacatcg ccgccgcgca ccagactaac atcagcggta acgaaattaa
     3421 ctccagttgc agagcgataa gacccaaaat cgaacccaac accgtaccgc caatacgttc
     3481 gaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca ccaccatggt
     3541 gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa aagtgagcag
     3601 gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata cccggatttc
     3661 aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaag attaaaagcg
     3721 ctcataactc atctgtcggc cagaaggcgc gcctctgaat tatccgacgg acataccggg
     3781 aacagtgtaa tttcccggca ggagcgaaaa atttgaaagg cgcatcttac tcttttcgct
     3841 ttcaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta gtgatgtttt
     3901 ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc attgttcaag
     3961 taaacctcga agcagggctt tggtaacatt tcataagcac tgtcctgcaa gaggctatta
     4021 aagaactgat accagggttt agcaaaatca tcaccgacta cacgagctac cgccaccgca
     4081 tactgaccac ctgaaatttc tgtcagaatg acgccctcac tgttttcggg aagcgtaaag
     4141 taacccggca ccgtcacgac ggtgccgcag cgtaattttt cggcgggtgt ttcatctgga
     4201 ttgtcgtaat agatagcaac ccactccttc ggcacaatat ttttactatc tacccacatc
     4261 atcaactgct caaaaccttt ctttaccgtc tgttcccagg ggccaacgag atggaaacct
     4321 gcaacggtac gtttatcttc ctgcttaatc tcgtagttca tgacgcctcc attaatactg
     4381 tttttatata cagtatagtt gcaatattaa aaccacaagg aatgagtgtt gattatgcga
     4441 gcagactcgc actcctgcca gtctgctgca aaagaaaggt caggccttat ggtggaaata
     4501 atcactcagg cgagaaaaca tgctgccttc cccgacagat tccagggtaa ccagcggcca
     4561 gtgcgccacc tgtttatcac ggtcgtaaag ttcaatttcc cctacccgct gatgggcgct
     4621 aattggcgcg gtgagttctt taccatcaag ggtatatttg gctttgatat gtggaatttc
     4681 ggctttcggt agcaccatcc agaactcttg ttccgttccc aggtcgatat tttctttatc
     4741 gccataccag atgcgttccg ttccgacctt tttcccacgg tgcaaaattt gcaccgtagt
     4801 aaagttttgt tgcccccaac gcagtaattt tcttgcctct tcctcacgac cttttgcact
     4861 gtcagccccc attatcactg caatgagacg atgctgccca tctacagccg aagcaatgag
     4921 attaaacccg gcaccagaag tatgacccgt tttcaggccg tcaacattca tggttttatc
     4981 ccacaataat ccgttacggt tttgctgggt gataccgttc caggtgagac ttttctcact
     5041 gtacatatga taaaactcgg gctcgccgtg gatgatagca cgagaaagca cagctaaatc
     5101 ataagccgag ctatgctggc caggtgcatc cagaccatgc actgtttcaa aatgcgtatc
     5161 cttgagatgc agcttctcgg catagttgtt catcatttca acaaactgcc gttgcccacc
     5221 ggcaatatag tcagccagag caacacaagc gtcatttccg gaatccacaa ttaaaccacg
     5281 gcttaaatca cgtaccgata cgcgatcgcc ctctttcaaa aacatcagtg aagaaccgac
     5341 aaacaccgga ttatctttcg cccacgcatc gcgccccacg gtgacaatat cgtctggcgt
     5401 aatgcgatga ctatcgatag cgcgatccac gacataaccc gtcatcagct ttgtcaggct
     5461 ggcgggattg cgctgttgat gctcattacc cgcggtgagg atctgaccgg tggtgtaatc
     5521 catcaatacc caggacccgg catgaatctc tggaggctga ggtgaaaaag gaatgttttc
     5581 cgccgcaaaa ccagacgata agttaaaaac gaacaaagaa gcagcaataa taagacggcg
     5641 tttcaacagc aaaccctcag gagtttcaaa tagctgttct ttttacggaa atacttatga
     5701 actggctgga ataaagtgca agaaaatgtg actaccctct catttttatc tgacatgatc
     5761 tgttgccact cgctgccaaa ttgtggcgct aaagctgatt agcacggtga tatttgatac
     5821 tctggcagac agcagaaata acggatttaa cctaatgatg aatgacggta agcaacaatc
     5881 tacctttttg tttcacgatt acgaaacctt tggcacgcac cccgcgttag atcgccctgc
     5941 acagttcgca gccattcgca ccgatagcga attcaatgtc atcggcgaac ccgaagtctt
     6001 ttactgcaag cccgcggatg actatttacc ccagcctgga gcagtattaa ttaccggtat
     6061 taccccgcag gaagcacggg cgaaaggaga aaacgaagcc gcgtttgccg cccgtattca
     6121 ctcgcttttt accgtaccga agacctgtat tctgggctac aacaatgtgc gtttcgacga
     6181 cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat ccttacgcct ggagctggca
     6241 gcatgataac tcgcgctggg atttgttgga tgttatgcgt gcctgttatg ccctgcgccc
     6301 ggaaggaata aactggcctg aaaatgatga cggtctaccg agctttcgcc ttgagcattt
     6361 aaccaaagcg aatggtattg aacatagcaa cgcccacgat gcgatggctg atgtgtacgc
     6421 cactattgcg atggcaaagc tggtaaaaac gcgtcagcca cgcctgtttg attatctctt
     6481 tacccatcgt aataaacaca aactgatggc gttgattgat gttccgcaga tgaaacccct
     6541 ggtgcacgtt tccggaatgt ttggagcatg gcgcggcaat accagctggg tggcaccgct
     6601 ggcgtggcat cctgaaaatc gcaatgccgt aattatggtg gatttggcag gagacatttc
     6661 gccattactg gaactggata gcgacacatt gcgcgagcgt ttatataccg caaaaaccga
     6721 tcttggcgat aacgccgccg ttccggttaa gctggtgcac atcaataaat gtccggtgct
     6781 ggcccaggcg aatacgctac gcccggaaga tgccgaccga ctgggaatta atcgtcagca
     6841 ttgcctcgat aacctgaaaa ttctgcgtga aaatccgcaa gtgcgtgaaa aagtggtggc
     6901 gatattcgcg gaagccgaac cgtttacgcc ttcagataac gtggatgcac agctttataa
     6961 cggctttttc agtgacgccg atcgcgcagc aatgaaaatt gtgctggaaa ccgagccgcg
     7021 taatttaccg gcactggata tcacttttgt tgataaacga attgaaaaac tgttgttcaa
     7081 ttatcgggca cgcaacttcc cggggacgct ggattatgcc gagcagcaac gctggctgga
     7141 gcaccgccgc caggtcttca cgcccgagtt tttacaaggt tatgcagatg aattgcagat
     7201 gctggcacaa caatatgccg ataacaaaga aaaagtggcg ctgttaaaag cgctttggca
     7261 gtacgcggaa gagatcgtct agtttgaagc ctccgccgct gatacggcgg aggacaatgt
     7321 tgggtcaggc tttttgaacg gtgatgctcc acgcagcatc gccaatctgc tgataatcgg
     7381 tgatggcatg tccttcttct gccgcccatt gtgggattgc ttctgttgcc tgggtgcaat
     7441 caaactcaat taccaactca tcgccgctgg ccattcctgc cagtgctgct tttgcttcta
     7501 tgagtggaaa cggacagacc tgcgtcacca catccagctt tttaatcgcc atctttacac
     7561 cttacttaat ttgccgcagc agttgccagt cgcgccttac gctgcggtcg aacatacaca
     7621 agccaggacg cagtccagac tccgagaatc ataaatacaa ggccaatcca gccctgccag
     7681 gtcatcattg cagtcataac cagtccatta ccgatagagc aaccacctgc aatactggca
     7741 ccgaagccca tcagtacacc acctaacccg ctacgtaatg ttgtttgtgc atcagctgcg
     7801 cgaacgcgga actcacggct cgctttggct gcaataaatg accccacgaa gatccctaac
     7861 accaggaaaa cgccccagtt aatgtatttc acgtcacccg cgaccagaaa ttgcagaata
     7921 ttggccgttg gggaagtgat tcctaaccca aacatgcgtc cggttgcttc actcaggggc
     7981 caggctaaaa gcgcaatcaa accgatgagt acagcagtga caaagggatg ccagcgtttt
     8041 tcaaacagaa tatgagcgat cccggttcgg cgcggcggta aggtcgcgac tttgagtttt
     8101 ggcttcttca actctttcat caccacccag agcgttatca ccagcaaaac ggcaaccaac
     8161 ggccacacag acaaattaaa agtctcagca atagagttat gttcagtact gtagtgctgc
     8221 aaggtttgat ttaaaccact ggcatgtgga gaacgcatca ccgcactcat caccatataa
     8281 gtgaaaagcg cgatccaact gccgatcaat ccctcacccg cgcgatacca ggtcccggtg
     8341 gcacatccac ccgccagaac aattcccagc ccaaagatat acccacctat aacagtacca
     8401 agccacggga acgccccggc ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc
     8461 cccacacttt gtaccgaaat tgcaatcagc aaggcgtaaa acatgcgatt atttttcgcg
     8521 agatacatat cgcgaaaacc acctgtcagg caaaaacgcc cacgctgcat gacaaatccc
     8581 agcagagcac cacaaattag cccgcttaat atcattgaaa acataaatta attaaccaga
     8641 tgaatgttaa tagggaaatt attcatgact ggtggaatgc agaccaataa ccaaattctc
     8701 taaattagaa caaatggtta ttaatgagga tgtcgataaa aaagaataaa aaaaccggag
     8761 ccaaatattc attcgactcc ggtttattat tacaaaagat gattacgcta cgtcttcgta
     8821 ctgcggaacc gggttacgga aactcttggt gacgcaagcc aggtaaatca gaccgatagc
     8881 cgcccagatc agacccagaa ccattgagct ttcttccagg ttaacccaca gcgcaccaac
     8941 ggtcagcgca ccacacatcg gcaggaacag atactggaag tgatctttca gcgtcttatt
     9001 acgcttctca cggatccaga actgcgagat gaccgacagg ttaacgaagg tgaacgccac
     9061 caacgcacca aagttaatca gcgctgtagc cattaccagg tcgaagttga ttgccagcag
     9121 ggcaatcgcg ccaaccagga tgatgttcat cgccggagta cgccatttcg ggtgtacata
     9181 accgaagaag cttttcggga atacgccgtc acgacccatt acgtacatca gacgcgctac
     9241 gcctgcatgc gccgccatac cggacgccag tacggtaatg gtggagaaga tcagcgcgcc
     9301 aacctggaac gctttacccg caacgtacag catgatttca ggctgtgacg catccggatc
     9361 tttaaagcga gagatatccg ggaagtacag ctgcaggaag taagttgcaa agatgaagat
     9421 catgccgcca atcagcgcgg tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt
     9481 ggtttcttcc gacaggttgc tgatgccgtc aaagccggta aaggagaagc acaggattgt
     9541 cgccccggta atcatcggga taacatgcgc atcgccagac cagaacggac gagtgctcgc
     9601 cagcgtacca gcgccttcac cttcaaatac gccataaaca accatgccca gaatcaccgc
     9661 gatcagcacc acctgcaaca cgacgattac agtgttgaag ttcgctacgg atttcagact
     9721 acgcaggtta aaggcggtca tgaaggccac caacgccacc acaaacatcc acgatgggat
     9781 ggaaggcacc agagcttcaa aatagatttt cgccagcaga atgttgatca tcggcgcgaa
     9841 cagatagtcg agcagagaag accaacccac cataaagccg acagtcgggc taatggattt
     9901 ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg cgaaccagct tcccgtagct
     9961 cagagccgta aacaggatcg caatcaatgc gaacgcatag gctgtcggca catgaccatc
    10021 cgtgaggcct gaaacgatac caaaagtatc aaacagcgtc atcggctgca tataggcaag
    10081 acccatcatt acaaccggaa ctaacgtaag cgttttacgc aattccacgc gagaggtgtt
    10141 tggagtaacg ttatgcgaca tggtcattct cctttacggt gataaccgtc gcgtaagcaa
    10201 aaaattgccc catttttttg gattcctcag cgacaacaac tgtcgatttt tagtaaatat
    10261 ctatccggta cgaagcccgg cctcttggta tgaatgattg gtttgaagca aaaaataacc
    10321 gacgctgatg aaacgtcggt ttttagtcat tttttgacag cggcgcattg tgcctaaatg
    10381 ggggggaaat gacaagagaa tgagaggctt gtcataataa tttttcttta aatggctgat
    10441 tttccgtcat cagattttgc ataaacaccg gcgatcgcac tatttgctaa aatttcatcc
    10501 cgccaccagc cactggcaag tcctgcggtc tgttcattcc agccaatcca gacgggttca
    10561 aagagagttt gggcgaccac tttcttctcg attaacgcac cactatcgag aaaacgttgc
    10621 gccagataac ggggtaaata gccgcagccg aggccgctaa tttgcagctc cagcttggtt
    10681 ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa gttgtgaggc cgtagaagcg
    10741 cctgcctgcg cagtatcccc caccacaatc gcccgatatc gcttaatgat acgacggttt
    10801 aacggttctt cttccagggc taacggatga tgaggcgcta ccgcaaagac ttgttccaga
    10861 tcgcccaacc gcgagaaacc aaactcactg gaagatggtg gctcatgcat cgcgccaaca
    10921 ataatatccg ctcgcccctg agtcagggca tcccatgaac caccgagtac gccattgata
    10981 aacttcaggc gcgtcacgct atgatgttga taaaaagctt caatcagtgg cgcaagcaga
    11041 gaaaaaggaa acgtgtcgtc cacgccaatc accagttcgt tttcccagcc ttcatgcagt
    11101 ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa cttcccgccc tttctctaat
    11161 aacatttttc cggtgcgggt gaatttagcg cggtgtccgc tacgatcaag caattgaata
    11221 ttaaggtcgc tttccagctt gtgaacggtg taactcagag cggacggcgt cttatagagt
    11281 ttggccgacg ccgccgcaaa actgccttct ttttctaagg catcgaggat catcaacaca
    11341 tccagcagtg gtttcataca cgcccccttg cagtgcatgc ggcgtcctgc tggcgtgatc
    11401 gtttactcca gcggcattac cagcggatcg ggatactggt attcaaatcc cagttcatta
    11461 caaatccgac tgccatcaat aatcttacct ttgccgctgt ccagactatt tctgaactgc
    11521 ggaggctcca gccccagtaa acgggccatc tgcggataga aaacattacg cgcagggtga
    11581 gctggcgcac atatattata gatgtgtccg cctttaggtg cctgtaacaa cagagtgata
    11641 gcgccaataa catcttctaa atggactaaa ttaacaccat gttcaccatc aggcgcggtt
    11701 tttccggcaa agaagcgtcc gggatgacgt cccggtccga ccagccccgc aagacgcaga
    11761 atatcgaccg aagtaccggg taaattgtgc agccagtctt cgagttcttc taatactcgt
    11821 ccactgttgg tcaccggatt acgcggggtg gtttctttca ccgtgccttg cgcgtcgcca
    11881 tagacagacg ttgagctggt aaaaataatt cggggaatac gatgggccag cgcgctatcc
    11941 actaactctt gtaccgcttg taaatagaac tcatcgccgg ggccgctacg acgtgccgga
    12001 agcgtaatga ccagcgcatc ggcatccatc agggcatcca gatcgtcaga atcgcaaact
    12061 aactcaggct ccatgcgaag cagatagctg tcaatgccac tcattcgggc cgcttcgaca
    12121 ccatcttgtg tggttttact cccggtgact tgccagcctc gcgctgaaag tgacatcgcc
    12181 agcggcatgc ctaaccaccc taacccgaca attgcgacct ttttcatccg ttatctcctg
    12241 atacacctta gatctataag gctacgctag cgtatcaaaa ctgacaattc attctatgaa
    12301 tgaatctgtt caataatgat aacgacatgc tgcaatgagc agtgaatcgt cggtaaccgc
    12361 gtataccaga cggtgctcct ctgtaatgcg tcgggaccag aaacctgaca aattatgttt
    12421 caggggttct ggcttcccct taccttcaaa tggcgttctg cgggtatctt tgataagttc
    12481 attgatcttt ttaacaattc gcttatctgt ttcctgccag tacagataat catcccatga
    12541 ttcctcagac cagattagtt tcactcaata atgtcctttt ctgttccttt gcctgatttc
    12601 aggctatcga ttgagtccat caatctccgg gcgttagcgg gggaacgcag tagataagcc
    12661 gtctcttcca gcgagttgta ttcttcgagt gacatcagaa cacaagcctc tccattctga
    12721 cgagtaataa ggatcggggc atgatcttca acggctttca tcattgttgc cgacaaattc
    12781 tgacgcgctt cgctgtagct aattgtacgc atgtcaatct cctcttttgt acagttcatt
    12841 gtacaatgat gggcgttaat taactattta ttaattagtt tgtagatcaa ggtattgtca
    12901 gtgagacgaa aatccaggct tcgctatttt tggtgccgtc agcttagagg acagtcctct
    12961 tagccccctc ctttccccgc tcattcatta aacaaatcca tcgccataaa atatataaaa
    13021 aagcccttgc tttctaacgt gaaagtggtt taggttaaaa gacatcagtt gaataaacat
    13081 tcacagagac ttttatgata cgcgttcaat ttaaacacca ccatcatcac catcatcctg
    13141 actagtcttt caggcgatgt gtgctggaag acattcagat cttccagtgg tgcatgaacg
    13201 catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgcg gttgataacg
    13261 gttcagacag gtttaaagag gaataacaaa atgacagaca acactcgttt acgcatagct
    13321 atgcagaaat ccggccgttt aagtgatgac tcacgcgaat tgctggcgcg ctgtggcatt
    13381 aaaattaatc ttcacaccca gcgcctgatc gcgatggcag aaaacatgcc gattgatatt
    13441 ctgcgcgtgc gtgacgacga cattcccggt ctggtaatgg atggcgtggt agaccttggg
    13501 attattggcg aaaacgtgct ggaagaagag ctgcttaacc gccgcgccca gggtgaagat
    13561 ccacgctact ttaccctgcg tcgtctggat ttcggcggct gccgtctttc gctggcaacg
    13621 ccagttgatg aagcctggga cggcccgctc tccttaaacg gtaaacgtat cgccacctct
    13681 tatcctcacc tgctcaagcg ttatctcgac cagaaaggca tctcttttaa atcctgctta
    13741 ctgaacggtt ctgttgaagt cgccccgcgt gccggactgg cggatgcgat ttgcgatctg
    13801 gtttccaccg gtgccacgct ggaagctaac ggcctgcgcg aagtcgaagt tatctatcgc
    13861 tcgaaagcct gcctgattca acgcgatggc gaaatggaag aatccaaaca gcaactgatc
    13921 gacaaactgc tgacccgtat tcagggtgtg atccaggcgc gcgaatcaaa atacatcatg
    13981 atgcacgcac cgaccgaacg tctggatgaa gtcatcgccc tgctgccagg tgccgaacgc
    14041 ccaactattc tgccgctggc gggtgaccaa cagcgcgtag cgatgcacat ggtcagcagc
    14101 gaaaccctgt tctgggaaac catggaaaaa ctgaaagcgc tgggtgccag ttcaattctg
    14161 gtgctgccga ttgagaagat gatggagtga tcgccatgag ctttaacaca atcattgact
    14221 ggaatagctg tactgcggag caacaacgcc agctgttaat gcgcccggcg atctccgctt
    14281 ctgaaagcat tacccgcact gttaacgata ttctcgataa cgtgaaagca cgcggcgatg
    14341 aggccctgcg ggaatacagc gcgaagtttg ataaaaccac ggttaccgcg ctgaaggtgt
    14401 ctgctgagga gatcgccgcc gccagcgaac gcctgagcga tgaactaaaa caggcgatgg
    14461 cggtggcagt taagaatgtt gaaaccttcc acactgcgca aaaactgccg ccgatagatg
    14521 tagaaacgca gccaggcgtg cgttgccagc aggtcacgcg cccggtcgct tcagttgggt
    14581 tgtatattcc tggcggctcc gccccgctct tctcaacggt attaatgctg gcgactccgg
    14641 cgcgtattgc gggctgtaaa aaagtggtgc tgtgctcacc gccgccgatt gccgatgaga
    14701 tcctttatgc ggcgcagctg tgcggtgtgc aggacgtgtt taacatcggc ggcgcacagg
    14761 ccattgccgc gctggcgttt ggtacggaat ctgtgccgaa agtggacaaa atcttcgggc
    14821 cgggtaacgc ctttgtcacc gaagcaaaac gccaggtaag ccagcgtctg gacggtgcgg
    14881 cgatcgatat gcccgcaggc ccgtcggaag tgctggtgat tgctgacagc ggcgctacgc
    14941 cggatttcgt ggcttctgat ttgctttctc aggctgaaca cggcccggac tcacaggtga
    15001 ttttactgac gcccgatact gatatggcgc atcaagttgc cgaggccgtc gaacgccaac
    15061 tggcagaact gccgcgtgcc gaaaccgccc gccaggcact gagcgccagc cgcctgatcg
    15121 tgactaaaga tttagcgcag tgcgtagaga tctccaacca gtacggcccg gagcacctga
    15181 tcattcagac ccgcaacgcc cgcgaactgg tcgatggcat caccagcgcc ggttcggtat
    15241 ttcttggtga ctggtcaccg gaatcggcag gcgactatgc ctccggcact aaccatgttc
    15301 taccgactta cggttacacc gccacctgtt ccagcctcgg gctggcagat ttccagaagc
    15361 gcatgaccgt acaggaactg tcgaaagagg ggttctccgc gctggcttca accatagaaa
    15421 cactggccgc cgccgagcgc ctgaccgccc acaaaaatgc cgttactttg cgtgttaacg
    15481 cccttaagga gcaagcatga gcaccgtgac tattaccgat ttagcgcgtg aaaacgtccg
    15541 caacctgacg ccgtatcagt cggcgcgtcg tctgggcggt aatggcgacg tctggctgaa
    15601 cgccaacgaa tatcccacag ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta
    15661 cccggaatgt cagccgaaag cggtgattga aaattacgcg caatatgcag gcgtaaaacc
    15721 ggaacaggtg ctggtcagcc gtggcgcgga cgaaggtatt gaactgctga tccgcgcttt
    15781 ttgcgaaccg ggtaaagact ccatcctcta ctgcccacca acgtacggca tgtacagcgt
    15841 cagcgccgaa acgattggcg tcgagtgccg cacagtgccg acgctggaca actggcaact
    15901 ggacttacag ggcatttccg acaagctgga cggcgtaaaa gtggtctatg tttgcagccc
    15961 caacaacccg accgggcaac tgatcaatcc gcaggatttt cgcaccctgc tggagttaac
    16021 ccgcggtaag gcgattgtgg ttgccgatga agcctatata gagttttgtc cacaggcatc
    16081 gctggctggc tggctggcgg aatatccgca cctggctatt ttgcgcacac tgtcgaaagc
    16141 ttttgctctg gcgggccttc gttgcgggtt tacgctggca aacgaagaag tcatcaacct
    16201 gctgatgaaa gtgatcgccc cctacccgct ctcgacgccg gttgccgaca ttgcggccca
    16261 ggcgttaagc ccgcagggaa tcgtcgccat gcgcgaacgg gtagcgcaaa ttattgcaga
    16321 acgcgaatac ctgattgccg cactgaaaga gatcccctgc gtggagcagg tttttgactc
    16381 tgaaaccaac tacattctgg cgcgctttaa agcctccagt gcagtgttta aatctttgtg
    16441 ggatcagggc attatcttac gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg
    16501 aattaccgtc ggaacccgtg aagaaagcca gcgcgtcatt gacgccttac gtgcggagca
    16561 agtttgatga gtcagaagta tctttttatc gatcgcgatg gaaccctgat tagcgaaccg
    16621 ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct ttgaaccggg cgtgatcccg
    16681 gaactgctga agctgcaaaa agcgggctac aagctggtga tgatcactaa tcaggatggt
    16741 ctgggaacac aaagtttccc gcaggcggat ttcgatggcc cgcacaacct gatgatgcag
    16801 atcttcacct cgcaaggcgt acagtttgat gaagtgctga tttgtccgca cctgcccgcc
    16861 gatgagtgcg actgccgtaa gccgaaagta aaactggtgg aacgttatct ggctgagcaa
    16921 gcgatggatc gcgctaacag ttatgtgatt ggcgatcgcg cgaccgacat tcaactggcg
    16981 gaaaacatgg gcattaatgg tttacgctac gaccgcgaaa tcctgaactg gccaatgatt
    17041 ggcgagcagc tcactaaacg agaccgttac gctcacgtag tgcgcaacac caaagagacg
    17101 cagattgacg ttcaggtgtg gctggatcgc gaaggtggca gcaagattaa caccggtgtt
    17161 ggcttctttg atcacatgct ggatcagatc gccacccacg gcggtttccg tatggaaatc
    17221 aacgtcaaag gcgacctcta tatcgacgat caccacaccg tcgaagatac cggcctggcg
    17281 ctgggtgaag cgttaaaaat tgcccttggc gacaaacgcg gtatttgccg ctttggtttt
    17341 gtgctaccga tggacgaatg ccttgcccgc tgcgcgctgg atatctctgg tcgcccgcac
    17401 ctggaatata aagccgagtt tacctaccag cgcgtgggcg atctcagcac cgaaatgatc
    17461 gagcacttct tccgttcgct ctcatacacc atgggcgtga ccctgcacct gaaaaccaaa
    17521 ggtaaaaacg atcaccaccg tgtagagagc ctgttcaaag cttttggtcg caccctgcgc
    17581 caggccatcc gcgtggaagg cgataccctg ccctcgtcga aaggagtgct gtaatgaacg
    17641 tggtgatcct tgatactggc tgcgccaacc tgaactcggt gaagtctgcc attgcgcgcc
    17701 acggttatga acccaaagtc agccgtgacc cggacgtagt gttgctggcc gataaactat
    17761 ttttacccgg cgtaggcacc gcacaagcag cgatggatca ggtgcgtgag cgcgaactgt
    17821 ttgatctcat caaagcctgt acccaaccgg tgctgggcat ctgcttaggg atgcaactgc
    17881 ttgggcggcg cagcgaagag agcaacggcg tcgatttgct gggcatcatc gacgaagacg
    17941 tgccgaaaat gaccgacttt ggtctaccgc tgccccatat gggctggaac cgcgtttacc
    18001 cgcaggcagg caaccgcctg tttcagggaa ttgaagacgg cgcgtacttt tactttgttc
    18061 acagctacgc aatgccggtt aatccgtgga ccatcgccca gtgtaattac ggcgaaccgt
    18121 tcaccgcggc ggtacaaaaa gataacttct acggcgtgca gttccacccg gagcgttctg
    18181 gtgccgctgg cgctaagctg ctgaaaaact tcctggagat gtgatgatta ttccggcatt
    18241 agatttaatc gacggcactg tggtgcgtct ccatcagggt gattatggca aacagcgcga
    18301 ttacggtaac gacccgctgc cgcgcttaca ggattacgcc gcgcagggtg ccgaagtgct
    18361 gcacctggtg gatctgaccg gggcaaaaga tccggctaaa cgtcaaatcc cgctgattaa
    18421 aactctggtg gcgggcgtta acgttccggt gcaggttggc ggcggcgtgc gtagcgaaga
    18481 agacgtggcg gcgttactgg aagcgggcgt tgcgcgcgtg gtggtcggct ccaccgcggt
    18541 gaaatcacca gaaatggtga aaggctggtt tgaacgtttc ggtgccgatg ccttagtgct
    18601 ggcgctggat gtccgtattg acgagcaagg caacaagcag gtggcggtca gcggctggca
    18661 agagaattcg ggtgtgtcgc tggaacaact ggtggaaacc tatttgcctg tcggcctgaa
    18721 acatgtgctg tgtaccgata tctcgcgcga cggcacgctg gcaggttcta atgtctcttt
    18781 atatgaagaa gtgtgcgcca gatatccgca ggtggcattt cagtcatccg gcggcattgg
    18841 cgacattaac gatgttgctg ccctgcgtgg tactggcgtg cgcggcgtaa tagttggtcg
    18901 ggcattactg gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc aaaacgtata
    18961 attccgtgtc tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat
    19021 gaaatcattg gcgatatcgt gccgctggca aaacgctacg ccgaagaagg tgcagacgaa
    19081 ctggtgttct acgatatcac cgcttccagc gatggccgtg tggtagataa aagctgggta
    19141 tctcgcgtgg cggaagtgat cgacattccg ttctgtgtgg cgggtgggat taagtctctg
    19201 gaagatgccg cgaaaattct ttcctttggc gcggataaaa tttccatcaa ctcgcctgcg
    19261 ctggcggacc cgacgttaat tactcgcctg gccgatcgct ttggcgtgca gtgtattgtg
    19321 gtgggtattg atacctggta cgacgctgaa accggtaaat atcatgtgaa tcaatatact
    19381 ggcgatgaaa gccgcacccg cgtcactcag tgggaaacgc tcgactgggt acaggaagtg
    19441 caaaaacgcg gtgccggaga aatcgtcctc aacatgatga atcaggacgg cgtgcgtaac
    19501 ggttacgacc tcgaacaact gaaaaaagtg cgtgaagttt gccacgtccc gctaattgcc
    19561 tccggtggcg cgggcaccat ggaacacttc ctcgaagcct tccgcgatgc cgacgttgac
    19621 ggcgcgctgg cggcttccgt attccacaaa caaataatca atattggtga attaaaagcg
    19681 tacctggcaa cacagggcgt ggagatcagg atatgttaac agaacaacaa cgtcgcgaac
    19741 tggactggga aaaaaccgac ggacttatgc cggtgattgt gcagcacgcg gtatccggtg
    19801 aagtgttaat gctgggctat atgaacccgg aagccttaga taaaaccatc gaaagcggca
    19861 aagtcacctt cttctcgcgc actaaacagc gactgtggac caaaggcgaa acgtcgggca
    19921 atttcctcaa cgtagtaaat attgcgccgg actgcgacaa cgacacgcta ctggtactag
    19981 cgaatcctat cggaccaacc tgccacaaag gcaccagcag ctgcttcggc gacaccgctc
    20041 accagtggct gttcctgtat caactggaac aactgctcgc cgaacgcaaa tctgccgacc
    20101 cggaaacctc ttacactgcc aaactgtatg ccagcggcac caaacgcatt gcgcagaaag
    20161 taggtgaaga aggcgtggaa accgcactgg cagcgacggt acatgaccgc tttgagctga
    20221 ccaacgaggc gtctgatttg atgtatcacc tgctggtgct gttgcaggat caggatctgg
    20281 acttaacgac ggtgattgag aacctgcgta aacggcatca gtaagttgcg ggtgatcgga
    20341 tgcgatgttg ttgtcgcatc cggcaaaaaa aacgggcaag gtgtcaccac cctgcccttt
    20401 ttctttaaaa ccgaaaagat tacttcgcgt tgtaattacg cagagcgtta cgccccagca
    20461 caatccccgc accaaccatg ccgcccagca gtaccgccag aatcaaagtg attgcctttt
    20521 tcgggctatc gcgacgaata ggtaacgtcg gtttcatcac atagaggtaa gcatgaatat
    20581 caagatcatc aacttttaag ctttcgatat ccagcaggtt ttgacgtgtc tgatagtagt
    20641 ttggtgagaa caccaacgga cgagtcgctt catgcttaat catcgactcc agcgcttcgc
    20701 tccccaaaag gaacaacgta tcttgtgtca catcttcagt ctgctgaacc tgtggctttg
    20761 tcacctgcgc ctgattcgca tactgcaacg cttcctgaat ctgacggata cgcagatctt
    20821 tctgctcctg cgcaactact tcctgagttt tcagagaatc ctgcaagttt ttcatacgct
    20881 gcgcgatgtt gtctttcagg tcttttccca gttcttcatt tacctggtcg tcaacttgct
    20941 ggatatactt cgccagctgc ttctgagcac cttcaggtgt ttcaccaaca taagacaccg
    21001 ctaaaggtaa tgactgattt ttgacggtag gctcaatggt cagcttttct gcttcttcct
    21061 ggttatccag cgtttccgct aatgctgaga atgcagtgct gtaacgtccg ataagtgaag
    21121 cctgaatgtc ggagactttc ggtacagccg atccgtagat cacattcatc gcgttggtat
    21181 aaccagcgat ttgccccaca tcaggttgag taacgatagc tgtggaagtc cacttttctt
    21241 tggcaacggc cagataccct atagccaggg caatagcaac aatgacagag atgataattg
    21301 tcatcttgcc acgccacaac tgcaccagta aatcaatcaa atcaatctgt tccgggtcat
    21361 ggttttgccc agaaacatta ttattttcta ctctcatcat taccctaacg gacgaaataa
    21421 ttttaagtcg ccatagttta gcgataatcg ccatgaatgc tataggataa atgataaaaa
    21481 taatgaatta ttacaaaggg agcataagac aatcagggcg taaatagccc tgataacaag
    21541 atgttagtcg ctgccaaaga gatcgcgggt gtatacctta tctgccacat ctttaagctc
    21601 ttctgccatt cggttagaga taatgacgtc ggcttgttgt ttgaagatgg cgagatcacg
    21661 ttccaggcga gagttgaaga atgagtcttc tttcatcact ggctcgtaga tgatcacttc
    21721 aacgcctttc gccttgatac gtttcataat cccctgaata gaagacgcac ggaagttatc
    21781 tgaaccgctc ttcataatca gacgataaat acccaccact tgcggtttgc gtgacaaaat
    21841 ggcatcggca ataaaatcct tacgcgtgcg gtttgcatcg acaattgccg agatcaggtt
    21901 attcggcaca gactgatagt tcgccagtaa ctgcttggta tctttcggca aacaataacc
    21961 accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga gacaaacgcc
    22021 ttcgataatc tggcgagtat tcagacctaa actttctgca tagctatcca gttcattaaa
    22081 gtatgctacg cgcatcgcca gataggtatt agcaaacagt tttatcgctt ctgcttcagt
    22141 ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc
    22201 aaaacgttct gcgcgttctg aacgctcacc aatgacaata cgtgaaggat ggagattatc
    22261 gtaaagggct ttaccttcgc ggaggaattc aggggagaat ataatatttt cagtgcgata
    22321 tttcttatgc atcgctgcag taaaaccaac gggaaccgtt gatttgatca ccataaccgc
    22381 ataaggattt atctcaacta cgt
//
LOCUS       DAZFWP020000077        22163 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFWP020000077 DAZFWP020000000
VERSION     DAZFWP020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.077"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            86..1762
                     /gene="ybaL"
                     /locus_tag="ACHK0Z_RS14060"
                     /old_locus_tag="pgaptmp_002812"
     CDS             86..1762
                     /gene="ybaL"
                     /locus_tag="ACHK0Z_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU4502806.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGLVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1895..3199)
                     /gene="gsk"
                     /locus_tag="ACHK0Z_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             complement(1895..3199)
                     /gene="gsk"
                     /locus_tag="ACHK0Z_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU4502807.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            3351..4310
                     /gene="aes"
                     /locus_tag="ACHK0Z_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             3351..4310
                     /gene="aes"
                     /locus_tag="ACHK0Z_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU4502808.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(4307..5269)
                     /gene="hemH"
                     /locus_tag="ACHK0Z_RS14075"
                     /old_locus_tag="pgaptmp_002815"
     CDS             complement(4307..5269)
                     /gene="hemH"
                     /locus_tag="ACHK0Z_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU4502809.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(5505..6149)
                     /gene="adk"
                     /locus_tag="ACHK0Z_RS14080"
                     /old_locus_tag="pgaptmp_002816"
     CDS             complement(5505..6149)
                     /gene="adk"
                     /locus_tag="ACHK0Z_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU4502810.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(6330..8204)
                     /gene="htpG"
                     /locus_tag="ACHK0Z_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     CDS             complement(6330..8204)
                     /gene="htpG"
                     /locus_tag="ACHK0Z_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU4502811.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(8314..8919)
                     /gene="recR"
                     /locus_tag="ACHK0Z_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             complement(8314..8919)
                     /gene="recR"
                     /locus_tag="ACHK0Z_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU4502812.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(8919..9248)
                     /gene="ybaB"
                     /locus_tag="ACHK0Z_RS14095"
                     /old_locus_tag="pgaptmp_002819"
     CDS             complement(8919..9248)
                     /gene="ybaB"
                     /locus_tag="ACHK0Z_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU4502813.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(9301..11232)
                     /gene="dnaX"
                     /locus_tag="ACHK0Z_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             complement(9301..11232)
                     /gene="dnaX"
                     /locus_tag="ACHK0Z_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU4502814.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(9904..9968)
                     /gene="dnaX"
                     /locus_tag="ACHK0Z_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(11361..11912)
                     /gene="apt"
                     /locus_tag="ACHK0Z_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             complement(11361..11912)
                     /gene="apt"
                     /locus_tag="ACHK0Z_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU4502815.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(12065..12442)
                     /gene="ybaN"
                     /locus_tag="ACHK0Z_RS14110"
                     /old_locus_tag="pgaptmp_002822"
     CDS             complement(12065..12442)
                     /gene="ybaN"
                     /locus_tag="ACHK0Z_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU4502816.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12512..13039
                     /gene="priC"
                     /locus_tag="ACHK0Z_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             12512..13039
                     /gene="priC"
                     /locus_tag="ACHK0Z_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU4502817.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            13053..13214
                     /gene="rsmS"
                     /locus_tag="ACHK0Z_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             13053..13214
                     /gene="rsmS"
                     /locus_tag="ACHK0Z_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU4502818.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(13267..13365)
                     /locus_tag="ACHK0Z_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /pseudo
     CDS             complement(13267..13365)
                     /locus_tag="ACHK0Z_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13426..16788)
                     /gene="mscK"
                     /locus_tag="ACHK0Z_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             complement(13426..16788)
                     /gene="mscK"
                     /locus_tag="ACHK0Z_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU4502819.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(16916..17563)
                     /gene="acrR"
                     /locus_tag="ACHK0Z_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     CDS             complement(16916..17563)
                     /gene="acrR"
                     /locus_tag="ACHK0Z_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU4502820.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            17705..18898
                     /gene="acrA"
                     /locus_tag="ACHK0Z_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     CDS             17705..18898
                     /gene="acrA"
                     /locus_tag="ACHK0Z_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU4502821.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKARPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            18921..22070
                     /gene="acrB"
                     /locus_tag="ACHK0Z_RS14145"
                     /old_locus_tag="pgaptmp_002829"
     CDS             18921..22070
                     /gene="acrB"
                     /locus_tag="ACHK0Z_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU4502822.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         5062 a         5818 c         5914 g         5369 t
ORIGIN      
        1 tcaatgaaat tgtcataaac tattacctgt aattttggtt ttcccggcca aaaatggaca
       61 ataaccacca ggaaaaggag acggaatgca tcacgccacc ccgcttatca ccaccattgt
      121 tggcggcctt gtgctcgcct ttatcctcgg catgctggcc aataaactac gtatttctcc
      181 tctggtggga tatctgttag cgggtgtgct ggcaggacca ttcactccgg gctttgttgc
      241 cgataccaag cttgcgccgg aactggctga actgggcgtc attctgttga tgtttggcgt
      301 cggtttgcac ttttcgctga aggatttgat ggcggtaaag gccatcgcca ttcccggtgc
      361 gatcgcccag atagccgtgg cgacgctgct gggtatggcg ctctctgccg tgctgggctg
      421 gtcgttaatg accggtctcg tgttcggttt atgtctttcc accgccagta ccgtggtgtt
      481 actgcgcgca cttgaagaac ggcaattaat tgacagtcag cgtgggcaaa tcgccatcgg
      541 ttggttgatt gtggaagacc tggtaatggt tctgacgctg gtgttgctgc ccgcagtggc
      601 aggaatgatg gaacagggcg atgtgggctt tgccactctt gcagtcgata tggggatcac
      661 catcggcaaa gtgatcgcat ttatcgccat tatgatgctg gtaggtcgcc gtctggtgcc
      721 gtggattatg gcacgcagcg cggcaaccgg ttctcgcgag ctgtttaccc tgtcggtgct
      781 ggcgctggcg ttagggattg cctttggtgc ggtagagctg tttgatgtct cctttgcact
      841 cggtgcgttc tttgccggga tggtactgaa cgagtctgaa ctgagtcacc gtgccgccca
      901 cgatacgctg ccattgcgcg acgcgtttgc ggtgctgttt tttgtctccg tcgggatgtt
      961 gtttgatccg ttaattctga ttcagcaacc gctggcagtg ctggcgacgc tggcgattat
     1021 tctgtttggt aagtcgttag ccgcattttt cctggtgcga ctgtttggtc actcccaacg
     1081 tacggcatta accatcgccg ccagcctggc gcagattggt gagttcgcgt ttatcctggc
     1141 gggactggga atggcattga atttactgcc gcaggccgga caaaacctgg tactggcagg
     1201 ggcgatcctg tcgattatgc tcaacccggt actgttcgca ctactggaga aatatctggc
     1261 gaagaccgaa acgctggaag agcagacgct ggaagaggca atcgaagaag agaaacagat
     1321 cccagtggat atttgcaacc atgcgctatt ggtgggttac ggtcgtgtag gcagcctgct
     1381 gggggagaaa ttgctcgcct ctgatattcc gctggtggtg attgagacgt cacgaacccg
     1441 tgttgatgag ctgcgagagc gtggggtccg cgctgtattg ggcaatgcag cgaacgaaga
     1501 aattatgcaa ctggcgcatc tggaatgtgc aaaatggctg atcctgacga tccccaacgg
     1561 ttatgaagcg ggtgagattg tggcgtctgc ccgcgcgaaa aacccggata ttgagattat
     1621 cgcccgcgcc cattatgacg atgaagtgac gtatatcacc gaacgtggcg cgaatcaggt
     1681 agtgatgggt gaacgtgaaa ttgcccgtac tatgctggaa ctgctggaaa cgccaccggc
     1741 aggtgaagtg gtgacggggt aaacggtggt tgccggatgt aaaatatgaa aaatatgtag
     1801 gcatgataag acgcgccagc gtcgcatcgg gcatcgagag ccaaatgcca gatgcggcgt
     1861 gaacgcctta tctggcctac gaaccgacga taacttaacg atcccagtaa gactcttcca
     1921 ggctgtcttc acgctccggc aagccgcgcg ttaaacgagg tgaatgctgg ttcagtacct
     1981 gatagctcac acggttagca tatttacaca cctgcgctaa cgatgaataa gttaaccagg
     2041 tgaatttatg tttgctggag tttggtacgt tgctacgatg gtagctgttg gcggtaatgt
     2101 catgcagcaa cgctgccaat gcgccatccc ccgctccatt agtgttcatg attttttccg
     2161 gcccgcccat gtacggcgca atgtgcgaat atacacgcag cggattctgg caatccttgt
     2221 ggcgcatggc gcggctaaac tcatactggt tgaattccgc tatagcgccc ggcagcagcg
     2281 gatgctgggt tttacgtttc gcttcgtctt cggtaaagcc cgccatatac aagccgattg
     2341 gcccggcggt gcacagcacc agatctaccc agtccagcgc cttgtcagat gccaacaacg
     2401 gatcgctttc tccggtcaac gcttcggctt catcttcgtt catcgcaagg atagaaacgt
     2461 ggtctttgag gaattgctgc caccactgcg gattctcggc aatgacaaac ttggtgccca
     2521 gcgtcagcac caccggtacg ttatatttct tcgcgtactc aatggctttc atggttgctt
     2581 ccggcatggg ttcacccggt ttgcaacgca ccagataaga ggtgagaacc agtgccgagg
     2641 ctccggcaat cacatcttcc ggaatgcttt cagcccgcag ctggttcatg tggcctggac
     2701 tgatagcaaa ggtacgttcc ccggactcgc caatcagcgt aaagcaacga ccaatcgggc
     2761 catccacgcc ttgtagatag ttaagatcgg tacggctgga agtgttacac aggtaacgat
     2821 aggcataact gccaatttca atattgctgc acatgacgcc cagcagcacc gaacggtcgt
     2881 ccgcgagcac cgagtagttg tgcatggtgt taccaatggt gccacccgca aactgatggg
     2941 taatcaggtt tttctgtttt agctcctgat aaagcgcttc ggctacgtca tcctcaatca
     3001 ccagtgaatg cccggcgctt aatccataac gctcaatgaa ttcatcatcc actttcgctt
     3061 caatatcgac cagcgtctgg tcaataccta cgacccaggc ggcgctggtt tcgttttctg
     3121 gctggaattg ctgaagcagc ggatcgcgtg catttacagg gaagtaatgt ttggatttac
     3181 gtttaccggg aaatttcatg gttgttttta cgggtggtta actgagcggc gaatgttaac
     3241 acaatctgac gggagcgcgc gagacgcagc cagtttcaat atcagcaggc gggatcgcat
     3301 tttcatggca gtggttcctt acaatgacgt aatttgaaag gagtttttgt atgaagccgg
     3361 aaaacaaact acctgttctg gaccttattt ctgctgaaat gaagaccgtt gtgaatactc
     3421 ttcagccgga tttaccgtcc tggcccgcaa cgggaacgat tgctgagcaa cgacagtatt
     3481 acacgcttga gcgccgattc tggaatgcgg gcgctccaga aatggcaacc agagcttaca
     3541 tggttccaac aaaatatggg caggtggaaa cacgtctctt ttgtccgcag ccagatagcc
     3601 cggcgacgct attttatttg catggaggcg gttttattct cggcaatctc gatacccacg
     3661 atcgcatcat gcgcctgctg gcaagctaca gccaatgtac ggtgattggt attgattacc
     3721 ccctttcacc tgaagcgcgt tttccgcaag cgatagagga aattgtggct gcttgttgtt
     3781 atttccacca gcaggcggag gattatcaaa tcaatatgtc ccgcattggc tttgccggtg
     3841 attccgcagg tgccatgctg gcgctcgcca gtgcgttgtg gttgcgtgat aaacagatcg
     3901 attgcggtaa aattgcgggt gttttgctgt ggtatgggct ttacggatta cgggattccg
     3961 tgactcgtcg tctgttgggc ggtgtctggg atggcttaac gcaacaggat ttgcagatgt
     4021 atgaagaggc gtatttaagc aacgacgcgg accgcgagtc accgtattac tgtctgttta
     4081 ataatgatct cactcgcgaa gttccgccct gttttattgc cggggcggag ttcgatccgc
     4141 tgctggatga cagccgtctg ctttaccaga cgttagcggc gcatcagcag ccctgtgagt
     4201 tcaaactcta cccaggtacg ctgcatgcct ttttgcatta ttcacgaatg atgaaaaccg
     4261 ccgacgaggc tcttcgcgac ggcgctcagt tctttaccgc tcagctttag cgatacgcgg
     4321 caacaagatt agccatcatt tcgatatgtt ccggcgtggc attaagcgct ggaatatatt
     4381 catatttttt cccgccggca ccgaggaaga cctcacggtt ttgctcggca atctcttcca
     4441 gcgtctccag acaatccgca gcaaagcccg ggcacatcac ctgtatatga cctacgcctt
     4501 tttctccgag cattttcagc gtttcgtcgg tataaggcat cagccagggt tcccggccaa
     4561 agcgcgactg aaaggtcatc atcacttttt ccggtgccat ccccagtgcg gaagccagtt
     4621 cgcgagtcgt tgtgcggcaa cgttgcgggt aatcatcgcc ttcatctgca taacgctggg
     4681 gaatgccatg ataagagagc agcagcagat ccggttcgcc atgtttggca aaagaagcgc
     4741 gtacgctgtt cgccagtgca ttaatgtaat cgtggttatc ggcgtaatca cgaataaacg
     4801 atatccccgg aatgctacgt ttgcgcgcca gaatgcgtgc cagttcatcc cataccgcac
     4861 cgaccgtaga acaggagaat tgcggataaa gcggcagcac cacaatatga tctacatgct
     4921 ctgccaggag ttcatcgacg gcgctttcca gtgatggcga gccgtagctc atgcccagcg
     4981 ctacgggcgt ctccggtaaa cgttgtgcca gcgcctgctg ttgctggcgg ctgtaaacca
     5041 tcagcggcga gccgtcttcc atccagaccg aggcgtacag cttcgccaca cgcggcgagc
     5101 gcagcggcaa aatcacgccg cgcagcaacg gccaccataa caaccgtgag gtatcaacca
     5161 cgcgtctgtc gcttaaaaat tgtttcagat agcgttttac cgcttcaggt gtgggggcat
     5221 cgggcgtacc caggtttgcc agcaggatac cggttttagt ctgacgcatt accacctctt
     5281 atcgattcaa cttgttgata attgtagcgg aaaagtgagg aagaagaact aattgctgtg
     5341 agaggtcaaa tcagaaaaag gaatagcgca gattgaggtt tttgtaaaag gccgaatgaa
     5401 tatccggcct gagattgctg ataagtttgc ttgtgcgggc ctgttcccct caccctaacc
     5461 ctctccccga gggggcgagg ggactgtccg tgcgcgcttt cgaattagcc gaggattttt
     5521 tccagatcag cgcgaacttc agcaaccggc ttggtgccgt caactttcgc gtatttggtg
     5581 ttacccgctt ccgcttcttt ggagtagtag ccgatcagcg gtgcagtcat ctgatggtat
     5641 tcaaccagac gtttacgcac ggtttcttcc tgatcgtctt tacgggtagt cagttcttca
     5701 ccggtaacgt cgtctttgcc ttctactttc ggcggattga atttaacgtg ataaacacga
     5761 ccagacggcg catgaacgcg gcggcctacg atacgatcaa caatcagttc gtccggtacg
     5821 tcgaattcca gaacgtaatc aacattgatg cccgcttctt tcatcgcgtc tgcctgcgga
     5881 atggtacgcg ggaagccgtc caacaggaaa ccattacggc agtcttcctg agcaatgcgc
     5941 tctttaacca gcgcgatcac cagttcgtcg gtgaccagtt tgccagcatc cataatgtct
     6001 tttgcttgtt tacccagctc ggagccagat ttgaccgcag cacgcagcat atcgccagtg
     6061 gagatttgcg gaataccata tttctccatg atgaactgag cctgagtccc tttccccgcg
     6121 cccggagcgc caagcagaat gatacgcatt gcgaaaatcc ccttaaaatg tgtcgaattt
     6181 tttgaaaaag cgataaacga taccaccaac aggcgattgc ctcaagaaag gcacctgagg
     6241 ctgaaacggt taatggcgag ataatttgcg gaaagtgcag atgatgaaaa gaaaaatgcc
     6301 ggatgacaca aaggtcatcc ggcatgacat caggaaacca gcagctggtt catacgacga
     6361 ataaacaggt tcggatcttc cagcgtgccg cgttctgcca gcagcgcctg atccagcagc
     6421 agttctaccc actcgctgaa cttcgcttca tcttcagtat ctgccgcacg tttcaccagt
     6481 acgtgatccg ggttcagttc gaagatgtat ttcacttctg gcactttctg gcccgccgca
     6541 gcgaacagtt tcgccatctg agtgctcatt tcgtccgcgt cggtcgaaac gatcgctggc
     6601 gtatcggtca gacggtgagt cagacggaca tctttcacgc gctcgccgag cagggctttc
     6661 acacggtcga tgaacggagt cagtgctttc tccgcttctt tcgcgctctc atcaacttcg
     6721 tcagccagtt tttccagcga ctcgtcaact ttagacaccg actggaacgg tttaccgtcg
     6781 aactcagtca gatagttcat catccactca tcgatgcggt cggaaagcag cagaacttcg
     6841 atgcctttct tacgcagcag ttccaggtgc gggctgctct tcgctgccgc atagctgtct
     6901 gcagtgatgt agtagatttt ctcctgccct tctttcatgc gggaaacgta gtcttccaga
     6961 gacacggtct gcgcagaaga atcggtataa gtagaagcaa aacgcagcag tttggcgatc
     7021 gcttcctggt tagcgaaatc ttccgccgga ccttctttca gtaccaggcc aaactgttgc
     7081 cagaaggtct ggtatttgtc cgcgtcgtct ttcgccagtt tttccagcat ttgcagcaca
     7141 cgcttggtca gcgcattgcg caggttacgc gttaccgtgc tgtcctggag gatttcacgg
     7201 gaaacgttca gcggcagatc gctggagtca atcagaccac gcacgaagcg cagatagttc
     7261 ggcatgaact gttctgcgtc gtccatgatg aacacacgct ggacgtacag tttcaggccg
     7321 tgtttatgat cacggttcca catatcccac ggagcctggg acgggatgta cagcaggctg
     7381 gtgtactcct gcttaccttc aacgcggttg tggctccagg tcagcggatc attaaagtcg
     7441 tgggcgatgt gtttgtagaa ctctttgtac tcttcatcgg tgatttccga cttgttacga
     7501 gtccacagcg cctgcgcttt gttgattttc tcccaggaga taacggtttc gccgtctttc
     7561 tcttcgcgtt tttcgatctc taccggcagc gcgatatggt cggagtattt gctgatgatg
     7621 gaacgcacgc gccagtcatc gaggaactcg tcttcgcctt cacgcagatg cagggtgatt
     7681 tcagtaccac gatcttcttt ggtgatgtcg gcaacggtat attcaccttc gccagccgat
     7741 tcccagaaga cgccgttttc tggtttttcg cctgccgcgc gagtacgcac ggtcactttg
     7801 tcggccacga taaacgcaga gtagaaacca acaccaaact gaccgatcag ctggctgtct
     7861 ttcgcctggt cagaacccag ggattcgagg aatgatttgg taccggattt agcgatagtc
     7921 cccagatggt caatcacttc gtcgcgggtc atccccacgc cgttatcgga gatggtcagc
     7981 gtgcgcttgt ctttatcgaa agagacacga acgcgcagtt cgccatcacc ttcgtacagg
     8041 tccgggttag atagcgcacg gaaacgcagc ttgtccgccg catcggaggc gttagagata
     8101 agctcacgca ggaagatttc tttattggaa tagagagaat ggatcatcag gtgcagaagc
     8161 tgtttcactt ctgactgaaa accacgagtt tcttgtcctt tcatgtaggt ctacctcaat
     8221 aatgccattt taaggttaaa aacaggatga gggagagatg gggacaaggg agaataattt
     8281 caagcgagag caggtgatcc tgctctcgtt tgcttaaaaa cgaatcttat gacgcccggc
     8341 aagggagtgt gacagcgtgg tgccatcgac catttccagt tcgccgccaa ccggtacgcc
     8401 atgagcgatt cggctggctt ccacgtcata ttgcgcgcaa agctcggcaa tgtagttagc
     8461 ggtagcttca ccttcaaccg tgggattggt ggcgaggatc acttcagtga ttttttcctc
     8521 tgccagacgc tgttccagac gatcaaggcc gatatcatcc ggaccgatgc cgtccagcgg
     8581 tgacaggtgc cccatcaaca caaaataacg gcctgaaaac tgccccgtct gctcaatggc
     8641 gtagatgtcc gccggactct ccaccacaca gatttgaccg ttttcctgac gacgcggatt
     8701 cgaacagatg ttacagactt cctgttcggt gaaagtgcgg caatcggcgc agtggccgat
     8761 ttccgacatc gcccgggtga gcgcctgcgc cagacgcatc ccgccgctac gatcgcgctg
     8821 aagcagcgtg aacgccatac gctgcgccga cttcgggcca acgcccggca gacagcgcag
     8881 tgcttccata agctgtgtta acagcgggct ggtttgcatc agaacggcat cttaaagcca
     8941 ggcggcagct gcattccgga ggatacagag gccatttttt ctttctgcgt ttcttcaata
     9001 cgacgtgctg cgtcgttgaa tgctgcagcc accaggtctt ccagcatctc tttgtcgtct
     9061 tccagcaggc tcgggtcgat ctctacgcga cggcagttgt gtgcaccgtt gatggtcact
     9121 tttaccagac ctgcgccaga ttcgccggtg acttccagct gcgcgatctc ttcctgcatt
     9181 ttctgcattt tttcttgcat ctgctgggct tgcttcatca ggttacccag accgccttta
     9241 ccaaacatag gtttctctct caatcacgtt aaggatgacg aacgtaagct gtgcttacga
     9301 tcaaatgggg cggatacttt cttcatccag ctccgcatcg aagaaccgac gcagggtctg
     9361 aatattatta tccgcaataa tggactcgcg cgcctgcgca agtttttctt cgtatatcgc
     9421 ctgacgccac tccagcggcg tacgcaccgc gggattatca tcttcaacga tagtcagttc
     9481 aaccgttgaa ccttttaacg tgctcaacgc ttcagccagt ttttgctgtg caccgcggtt
     9541 gttcaaatgc cgctgagagg agcgcaaatg cagacatact gcgttgtcgc tctcctcttt
     9601 ccaggcattt aaggccacct gttcgaccag ttttggtagc gaaagttgac tcacctgtgc
     9661 cgcccacgcg tcgcgctcaa tggcttctgc cgccagcttc gccgccagtt ccggcgtttt
     9721 ttcatgttcc agcgcttttt tcagcgcctt cggcgtggcg accacttctt tttgctgcat
     9781 cactggagtg gtagccttcc agcgatacgc ttcttttttg gctggcgctt tttccagcgc
     9841 cgatggcacc ggacgcgcct gaacgcgatc ggtgaccgaa gccagtcttt ccagcgcagc
     9901 gttattcacc ggccgcgcgc gggtagcggc tgccggttca ctcttttttg ctttggttgc
     9961 tccctgcacg cgctgcaact gctggcgcgc cgccagcacc tggctggtgg tttccgggag
    10021 cggtacagtc ggtgcctgct gcggcgctga ttgcggttgc ggcggcacct gggttggcgt
    10081 cattactgcc gttggcgcga cgggcgcaaa ggactggcgt ggcacttctg gctcaggcag
    10141 aggcatacgt ggatggaacg ccagcgcgcg cagcagcgtc atctcaacgc ccatgcggcg
    10201 gtccggcgca tacggtaatt ctttgcgacc aatcaacagc gtctgatagt aaagctgaat
    10261 atccgtcggc ggtatggtgc gcgccagttc acgcatccgc agctcaatgg cggccatgtc
    10321 gttgccaagt gcagcaggcg aaagttgtac catcgcaata cgatgcaaca ggccgagcat
    10381 ttccaccagc aacgcttccc actcgatacc acgggcagcg gcttcattaa tcagcgccat
    10441 tacgcgctcg ccgttggcct cgaccatcgc ttcaaccagc gacagcgcct gatcgtcgtc
    10501 aagcgtaccc agcatcgcac tgactgcctg ggttgaaacc tggccgtcac cgctggcaat
    10561 cgcctggtcg gtcagactta aggcatctcg caggctgcct tcagcggcgc gcgccagcaa
    10621 ttgcaacgcc cgcggctcgt gagcgatatg ttcttcgttg aggatgtgct caagttgatg
    10681 gcgaatttgc tcgacatcca gcgccttgag atgaaattgc aaacagcgcg acaaaatcgt
    10741 caccggcaat ttctgtggat cggtcgtcgc cagcagaaac ttaacgtgct ccggcggctc
    10801 ttcaagggtt tttaacagtg cgttaaagct gtggcgcgac agcatatgca cttcgtcgat
    10861 cagataaact ttgaaacgac cacgcgccgg agcgtactgg acgttatcca gcaggtcgcg
    10921 ggtatcttca actttggtgc gcgaggcggc gtcgatttca atcagatcga caaagcgccc
    10981 ctgctcgatt tcacgacagt tatcgcacac gccgcacggc gtcgcggtaa tgccggtttc
    11041 gcagtttagc cccttcgcca gcagtcgggc gatagaggtt tttccgacgc cacgggtacc
    11101 ggaaaaaaga taagcatgat gaatacgccc taacgacaag ccgttcgcca gtgcggtcag
    11161 cacatgttcc tggccgacga cgtcagcaaa ggtttgtggg cgccattttc gggctaagac
    11221 ctgataactc attggcaggc tctgaaacgc tggaaggtgg attcacgaag gggtaatgct
    11281 aacacagccc cgtcagaacg gcgaggctga atgcacatct tgtgctgtcc gtagcgtggg
    11341 cagcacaaga ctggcgataa ttaatggccc gggaacggga caaggctgta gctggtaatg
    11401 ccctgttttt cgagacgctg ttcgccgccg agatcgaaca ggttgataat gaacgcagcg
    11461 tcagccactt caccacccag acgacggatc agtttaacgg tcgcttcgat agtgccgccg
    11521 gttgccagca gatcgtccac caccagaact ttgtcgcccg gtttgatggc atcaacgtgg
    11581 atctccagct gatcggtgcc gtattccagg tcgtaagttt cactgatggt ttcacgcggc
    11641 agtttgcccg gtttacggac cggtacaaag ccaacgccca gacccagagc taccggagcg
    11701 ccaaacaaga agccacgcgc ttcggtgccg acaactttgg taatgcccgc atttttgtaa
    11761 cgctcaacca gcaagtcgat gctgagagcg taagctttcg ggtcttccag taagctggtg
    11821 acatcgcgga aaagaatgcc gggttttggg tagtcctgaa tgcttttgat gctatttttg
    11881 agatactcaa gctgctgtgc agtcgcggtc ataagtgtat gcctgcttgt tacggtggta
    11941 ctcacggcgc gttttaaacg tatcaaaaat aacagttgtt taacctttga ccgcctgcgc
    12001 ctgtgctcga aaacgccaga atgtactggc tgtacgcaat aattgcaact gcgattgttg
    12061 tgcttcagtg cttttcttgc ttttcatcaa tcaccggaat tcgccacata tagaaaagca
    12121 aacaggcgag aattaccagc aacatgatgc gcacccatgg catctggacg aaccacagag
    12181 aaatggcaaa cgtgagcaaa ataagcaaaa tcgcccgcgg tttgacgccg cgcggcatcg
    12241 catgatgttt ctgccagaaa cgtagatagc tgccaaacca tgagcggtac agcaaccagg
    12301 cgtgaaagcg cggggaagaa cgggcaaagc accaggccgc cagcaggata aacggcgtcg
    12361 tcggtaatac cggtaatacc acgcccagcg tacccagcac taccgccagc cagccaatga
    12421 tgattaaaat gattcgttgc atattgagtg ttgtcagctt acagagtggc tactttagca
    12481 taacaattat cattttcatt gaggtcttat cgtgaaaacc gccctgctcc tggaaaaact
    12541 ggaaggacag ctcgctacgc tgcgtcagcg ttgtgccccg gtagcgcaat ttgccacgct
    12601 aagtgcccgt tttgacagac acctttttca aacccgtgcg acaacactgc aggcttgtct
    12661 cgatgaggcg ggcgataatc tggctgcgct tcgtcatgca gttgagcagc aacagctgcc
    12721 gcaagtggcc tggctggcgg aacatctggc ggcacaactg gaagccatcg cgcgtgaagc
    12781 ctccgcctgg tcattgcgcg agtgggacag tgcgccaccg aaaattgccc gctggcagcg
    12841 taaacgtatt cagcatcagg attttgagcg gcggctacgt gagatggttg ccgaacgcag
    12901 agcccgcctg gcgcgggtga ccgatctcgt ggaacagcaa acgctgcatc gtgaagtgga
    12961 agcctatgaa gcgcgcctgg cacgctgccg ccatgcgctg gaaaaaatcg aaaacaggct
    13021 agcgcgctta acccgctagc aatggagaga gtatgtcact ggaaaatgcc cctgacgatg
    13081 tcaaactggc tgtcgatttg attgtgctgc tggaagaaaa tcagatccca gcccgcaccg
    13141 tgctgcgcgc gctggatatt gtaaaacgcg attatgaaaa gaaattaacg cgcgatgatg
    13201 aggcggaaaa gtgagaaata aatggggccg ctaaatgtcc cctaagattg tgggcaaacg
    13261 caaaactgcc tgatgcgcta cgcttatcag gcctacaccg ctcctgcaat acattgaatt
    13321 cttgcggttt cgtaggccgg ataaggcggt cacgccgcat ccggcatgaa caacgcgcac
    13381 tttgtcagta atctcggggc aactaaatgt cgcccccttt ggttattacc ctaccgctgg
    13441 cgtcgggtca tcgcctttgt agtcgcgttt gacttccgtc acctcatcgc ccttctcgtt
    13501 gtgcagatgc acttcaagct ggttaaaggc aatgttgatg tcgttttcac ggcacagctg
    13561 atcgatagta cggttcagct catcgacagt acgactacgg tcacgcagtt cacgcacata
    13621 cagacgcagc tcatgatcca acgtgctggc accaaacgcc gtaaagaaga cttccggcat
    13681 tggttcgtgc atcacccttg ggtgctcagt cgccgccttc agtaacactt tacgcacttt
    13741 ttccagatcg gagccatagg ccacgccgag acggatcacc agacgcgtag tagtgtcagt
    13801 caacgaccag ttgatcagac gctcggtaac aaacgctttg ttcgggatga tcacttcttt
    13861 gcgatcgaaa tcggtaatcg ttgtcgcacg aatacggatc ttacttaccg tccccgagaa
    13921 gctaccaatg gttaccgtat cgccaatacg caccggacgt tcgaacagaa tgatcaaacc
    13981 ggagacgaag ttaccgaaaa tttcttgtaa accaaaacca agacctacgg ataatgctgc
    14041 ggccagccac tggagtttat cccaagagac gcccagcgat ccgaacaccg tcatcgcacc
    14101 aacggcaata atgatgtagt taaggatggt agtaatggca tacgacgcgc cctgacgcat
    14161 attcagtcgc gagagcacca gcacttccag taaaccaggc aggttgcgaa tcaacgccca
    14221 ggccaccatt gaggcgataa tcgcaaacaa cagactgccc atggtgacgt ttttcaccac
    14281 cgcagcgcca gcttcagtgc cgttgtaatg ccagagcgtg atgctgtcga gatagctgaa
    14341 cacggtgatc aaatcggacc aaattgccca gaacatgaca ccgaacagcg caaacatcag
    14401 caacatggta atacgcagcg tctgctggtt aacctgttcc agtgcaatgg tgggttcttc
    14461 cggcggttcg gcaccttctg cgccctcttt caccagattc tgccgacgcg ccagcgcacg
    14521 acgccaggcg atacgccgcg ccgctacgct taagccgcgc aataccgtct ggtagagcaa
    14581 gttccagatg atcaccagat aaacggtttc aatccaacgc ccggagaggc gcagcgtggt
    14641 gtagaaataa cccgtggcag tcagcaccat caatgcaatc gggataatcg acagcacggt
    14701 aatggtgacc agtcgcatgg tgtgcgactc tttatcacgc cagctttcgc ggcacattgg
    14761 ccataccagg aaggcaatca gcagcaggtt gaagaaaatc atcgcctgcc ccagcacatc
    14821 atccatcaga tgcagcgggg aaagttctgc caccacagac cagaaatgga taggcagcaa
    14881 cgcgagactg atgcggacaa tttgccgacg ccagtggctg gtctgctgct ccggcatgcc
    14941 gaagtgacgc acggcaacgc catttttctc cagtaccttc cagcacaggc caaacaccag
    15001 ccagaaaatc gccagttttt tactgaacga ccatagcagt tcgctgatgt tgagctgcat
    15061 ggtcaacaga atcaggccaa ccgcgagaat aatcaggcac accggcagcg cgcggatcag
    15121 gtcgataagg atcgcttttg gtgtgttgag ctggctatcg ttacgcaggg aacccacagc
    15181 ggaagccagt ttctgttgat acgctttcag ccagcccaga cgccagtgga tcagcccggc
    15241 aatcaacagc agcggcaaac cggcgaggaa agcgataaaa acggcaggcc aggctttttc
    15301 ccagttcacc gtgattttca tcgacttaaa ttcatctttc aggctttgcg ggaacgcttt
    15361 gatccagtcc cagtccattg gacggttact gttcacccaa aagatttgct gagtcaggat
    15421 ggatttcagg tttttcgata cgctcattaa ctgctgctgg ttgatttgca ggttaatggc
    15481 catcatcagc tggttaccca actgtttgtt gagttgatcc agcaattcgc gacgcatatc
    15541 aaccacttgc aataacgcat cgtgaacttc gctgttgact tcgttggtgt gaccttcttc
    15601 cagtttgttg acgaacgcat cgctctggaa gagtgcatca cgctgctggt taacttcaaa
    15661 ctgttcgaga cgcaaatccg cgatgcggtt ggtcatgttt tccagttcat ccgccgaggg
    15721 cagcgtttgt tgttgctggt aaaggatacg agacaacagc aggctgccct tcaggacggc
    15781 aatctgctct ttaatattgc gttccgattg cagcgcccgc tccagccagt ttttgacttt
    15841 aatgttttgc tgcatcaact gattaccgtt ttcagtcgcg gtaatcagac gctgacttaa
    15901 ctgctggtta atttccagtt cctgtttcac cagcggatta gcctgaatac gcgcggcttc
    15961 atccggggag acggcttcct gcgccgtttt ttcagttaaa gtcaggcgct tgctgtttac
    16021 cgcttcttgc aacagctgca actggtgctc cagacgggcg ctgttcgccg tcacgtaatc
    16081 acgttgcttt tgcaaggtat cctgcaagac ggtgttccct tccaggcttt tacgctgctg
    16141 gtcaatctcc gcattcagca acgcctgctg agcctgcatt aacactttct ggctgggacg
    16201 taaggcagtc tcgccgacat cagtcccatc cagacgactg cgaatttgtt gcagctgctg
    16261 cgaagcgtta tacatcgcat tttgcacgcg ttcgggctgc gtctgtaacg aaaccagctg
    16321 gctgttataa gacgccagat cgttttgtgc gttttgcaaa tcgtccagcg cctgggcaac
    16381 gcgagtttcc agctggcgca acgaaagcgt actcagaatt ttgcgcgttt cttcgtcgtt
    16441 atcgacatcg ctaagtgctg ttaacgccgc ggtcgcctgg cgcatttttt ccggcgcttc
    16501 agcgactttt tgccgtagct gaactgtctc ttcttttatg cgatcgattt tatcgagggt
    16561 ggctaatgta tctgtcagat cctgctgcac cagtttgtcc tgagcagaaa gatctttttg
    16621 tttatttagt gagtcaagtt gcgcctgcag gtccgctttt gtcggcagat caccattcga
    16681 tgacgcccgc gcaaacgccg tgttctggca taacaagaca aaaacaaaag caataaatgc
    16741 tgaaaaaaca aaatgccgtg atcgtttgta atactggaac atagtcatga tgaatgaagg
    16801 tttctgaacc tgaagaacga cctgaaaaag tcaaaccgca agaatatcac gacgcagtga
    16861 accagaatag caacgacgaa aatgtccagg aaaaatcctg gagtcagatt cagggttatt
    16921 cgttagtggc aggattacga agcgtggggc acaggagata catctccagt aagatggcga
    16981 cgtaatcgcg ggcttctttt ttaagatcaa aagattgcgg ggcaaagagc cagttttcca
    17041 tcaggccgga aatatagccg cgcataataa ttgctgcccg acgcgtcatt aaatccgcag
    17101 gcaacatttt cgcttcaata caatgtttta acgtttgttc tatacggtca taactttcca
    17161 gacagagatt acgttgtgcc tgttgcacaa cagccatttc tccgacaaat tcgcatttgt
    17221 ggaatataat ctccatcaat aatcgacgcc gttcttctgt caccgtggat tcaagaacat
    17281 gaattaatat ctctcttaat actgagagtg gatcgccagg gaattttgcc tgatactcaa
    17341 gctctagttc accaatattg gattctgaca gttcccagat ctcactgaac aaatccgact
    17401 tgtctttaaa atgccagtag attgcaccgc gcgtaacgcc agctgctttt gcaatctcgc
    17461 ccagcgaggt ggatgatacc ccctgctgtg agaaaagacg tagagccaca tcgaggatgt
    17521 gttggcgcgt ttcttgcgct tcttgtttgg tttttcgtgc catatgttcg tgaatttaca
    17581 ggcgttagat ttacatacat ttgtgaatgt atgtaccata gcacgacgat aatataaacg
    17641 cagcaatggg tttattaact tttgaccatt gaccaatttg aaatcggaca ctcgaggttt
    17701 acatatgaac aaaaacagag ggtttacgcc tctggcggtc gttctgatgc tctcaggcag
    17761 cttagcccta acaggatgtg acgacaaaca ggcccaacaa ggtggccagc agatgcccgc
    17821 cgttggcgta gtaacagtca aaactgaacc tctgcagatc acaaccgagc ttccgggtcg
    17881 caccagtgcc taccggatcg cagaagttcg tcctcaagtt agcgggatta tcctgaagcg
    17941 taatttcaaa gaaggtagcg acatcgaagc tggtgtctct ctctatcaga ttgatcctgc
    18001 gacctatcag gcgacatacg acagtgcgaa aggtgatctg gcgaaagccc aggctgcagc
    18061 caatatcgcg caattgacgg tgaatcgtta tcagaaactg ctcggtactc agtacatcag
    18121 taagcaagag tacgatcagg ctctggctga tgcgcaacag gcgaatgctg cggtaactgc
    18181 ggcgaaagct gccgttgaaa ctgcgcggat caatctggct tacaccaaag tcacctctcc
    18241 gattagcggt cgcattggta agtcgaacgt gacggaaggc gcattggtac agaacggtca
    18301 ggcgactgcg ctggcaaccg tgcagcaact tgatccgatc tacgttgatg tgacccagtc
    18361 cagcaacgac ttcctgcgcc tgaaacagga actggcgaat ggcacgctga aacaagagaa
    18421 cggcaaagcc aaagtgtcgc tgatcaccag tgacggcatt aagttcccgc aggacggtac
    18481 gctggaattc tctgacgtta ccgttgatca gaccactggg tctatcaccc tacgcgctat
    18541 cttcccgaac ccggatcaca ctctgctgcc gggtatgttc gtgcgcgcac gtctggaaga
    18601 agggcttaat ccaaacgcta ttttagtccc gcaacagggc gtaacccgta cgccgcgtgg
    18661 tgatgccacc gtactggtag ttggcgcgga tgacaaagtg gaaacccgtc cgatcgttgc
    18721 aagccaggct attggcgata agtggctggt gacagaaggt ctgaaagcag gcgatcgcgt
    18781 agtaataagt gggctgcaga aagcgcgtcc tggtgtccag gtaaaagcac aagaagttac
    18841 cgctgataat aaccagcaag ccgcaagcgg tgctcagcct gaacagtcca agtcttaact
    18901 taaacaggag ccgttaagac atgcctaatt tctttatcga tcgcccgatt tttgcgtggg
    18961 tgatcgccat tatcatcatg ttggcagggg ggctggcgat cctcaaactg ccggtggcgc
    19021 aatatcctac gattgcaccg ccggcagtaa cgatctccgc ctcctacccc ggcgctgatg
    19081 cgaaaacagt gcaggacacg gtgacacagg ttatcgaaca gaatatgaac ggtatcgata
    19141 acctgatgta catgtcctct aacagtgact ccacgggtac cgtgcagatc accctgacct
    19201 ttgagtctgg tactgatgcg gacatcgcgc aggttcaggt gcagaacaaa ctgcagctgg
    19261 cgatgccgtt gctgccgcaa gaagttcagc agcaaggggt gagcgttgag aaatcatcca
    19321 gcagcttcct gatggttgtc ggcgttatca acaccgatgg caccatgacg caggaggata
    19381 tctctgacta cgtagcggcg aatatgaaag atgccatcag ccgtacgtcg ggcgtgggtg
    19441 atgttcagtt gttcggttca cagtacgcga tgcgtatctg gatgaacccg aatgagctga
    19501 acaaattcca gctaacgccg gttgatgtca ttaccgccat caaagcgcag aacgcccagg
    19561 ttgcggcggg tcagctcggt ggtacgccgc cggtgaaagg ccaacagctt aacgcctcta
    19621 ttattgctca gacgcgtctg acctctactg aagagttcgg caaaatcctg ctgaaagtga
    19681 atcaagatgg ttcccgcgta ctgctgcgtg atgtggcgaa gattgagctg ggtggtgaga
    19741 actacgacat catcgcagag tttaacggcc aaccggcttc cggtctgggg atcaagctgg
    19801 cgaccggtgc aaacgcgctg gataccgctg cggcaatccg tgctgaactg gcgaagatgg
    19861 aaccgttctt cccgtcgggt ctgaaaattg tttacccgta cgacaccacg ccgttcgtga
    19921 aaatctctat tcacgaagtg gttaaaacgc tggtcgaagc gatcatcctc gtgttcctgg
    19981 ttatgtatct gttcctgcag aacttccgcg cgacgttgat tccgaccatt gccgtaccgg
    20041 tggtattgct cgggaccttt gccgtccttg ccgcctttgg cttctcgata aacacgctaa
    20101 caatgttcgg gatggtgctc gctatcggct tgttggtgga tgacgctatc gttgtggtag
    20161 aaaacgtgga gcgtgttatg gcggaagaag gtttgccgcc aaaagaagct acccgtaagt
    20221 cgatggggca gattcagggc gctctggtcg gtatcgcgat ggtactgtcg gcggtattcg
    20281 taccgatggc cttctttggc ggttctactg gtgctatcta tcgtcagttc tctattacca
    20341 ttgtttcagc aatggcgctg tcggtactgg tagcgttgat cctgactccg gctctttgtg
    20401 ccaccatgct gaaaccgatt gccaaaggcg atcacgggga aggtaaaaaa ggcttcttcg
    20461 gctggtttaa ccgcatgttc gagaagagca cgcaccacta caccgacagc gtaggcggta
    20521 ttctgcgcag tacggggcgt tacctggtgc tgtatctgat catcgtggtc ggcatggcct
    20581 atctgttcgt gcgtctgcca agctccttct tgccagatga ggaccagggc gtatttatga
    20641 ccatggttca gctgccagca ggtgcaacgc aggaacgtac gcagaaagtg ctcaatgagg
    20701 taacgcatta ctatctgacc aaagaaaaga acaacgttga gtcggtgttc gccgttaacg
    20761 gcttcggctt tgcgggacgt ggtcagaata ccggtattgc gttcgtttcc ttgaaggact
    20821 gggccgatcg tccgggcgaa gaaaacaaag ttgaagcgat taccatgcgt gcaacacgcg
    20881 ctttctcgca aatcaaagat gcgatggttt tcgcctttaa cctgcccgca atcgtggaac
    20941 tgggtactgc aaccggcttt gactttgagc tgattgacca ggctggcctt ggtcacgaaa
    21001 aactgactca ggcgcgtaac cagctgcttg cagaagcagc gaagcaccct gatatgctga
    21061 ccagcgtacg tccaaacggt ctggaagata ccccgcagtt taagattgat atcgaccagg
    21121 aaaaagcgca ggcgctgggt gtttctatca acgacattaa caccactctg ggcgctgcat
    21181 ggggcggtag ctatgtgaac gactttatcg accgcggtcg tgtgaagaaa gtttacgtca
    21241 tgtcagaagc gaaataccgt atgctgccgg atgatatcgg cgactggtat gttcgtgctg
    21301 ctgatggtca gatggtgccg ttctcggcat tctcctcttc tcgttgggag tacggttcgc
    21361 cgcgtctgga acgttacaat ggcctgccat ccatggaaat cttaggccag gcggcaccgg
    21421 gtaaaagtac cggtgaagca atggagctaa tggaacaact ggcgagcaaa ctgcctaccg
    21481 gtgttggcta tgactggacg gggatgtcct atcaggaacg cctctccggc aaccaggcac
    21541 cttcactgta cgcgatttcg ttgattgtcg tattcctgtg tctggcggcg ctgtacgaga
    21601 gctggtcgat tccgttctcc gttatgctgg tcgttccgct gggggttatc ggtgcgttgc
    21661 tggctgccac cttccgtggc ctgaccaatg acgtttactt ccaggtaggc ctgctcacaa
    21721 ccattgggtt gtcggcgaag aacgcgatac ttatcgtcga attcgccaaa gacttgatgg
    21781 ataaagaagg taaaggtctg attgaagcga cgcttgatgc agtgcggatg cgtttacgtc
    21841 cgatcctgat gacctcgctg gcgtttatcc tcggcgttat gccgctggtt atcagtactg
    21901 gtgctggttc cggcgcgcag aacgcagtag gtaccggtgt aatgggcggg atggtgaccg
    21961 caacggtact ggcaatcttc ttcgttccgg tattctttgt tgtggttcgc cgccgcttta
    22021 gccgcaagaa tgaagatatc gagcacagcc atactgtcga tcatcattga tacaacgtgt
    22081 aatcactaag gccgcgcaag cggccttttt tatgcataac ctacgaacat taaggagtaa
    22141 ttgaaccacc aactcaggat ctc
//
LOCUS       DAZFWP020000078        22041 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFWP020000078 DAZFWP020000000
VERSION     DAZFWP020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.078"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            430..1329
                     /gene="yegS"
                     /locus_tag="ACHK0Z_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             430..1329
                     /gene="yegS"
                     /locus_tag="ACHK0Z_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU4502823.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1411..2184)
                     /locus_tag="ACHK0Z_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             complement(1411..2184)
                     /locus_tag="ACHK0Z_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4502824.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2290..3330)
                     /gene="gatD"
                     /locus_tag="ACHK0Z_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             complement(2290..3330)
                     /gene="gatD"
                     /locus_tag="ACHK0Z_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4502825.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3378..4733)
                     /locus_tag="ACHK0Z_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             complement(3378..4733)
                     /locus_tag="ACHK0Z_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU4502826.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4737..5021)
                     /gene="gatB"
                     /locus_tag="ACHK0Z_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             complement(4737..5021)
                     /gene="gatB"
                     /locus_tag="ACHK0Z_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU4502827.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARGDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(5052..5504)
                     /gene="gatA"
                     /locus_tag="ACHK0Z_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             complement(5052..5504)
                     /gene="gatA"
                     /locus_tag="ACHK0Z_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU4502828.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5514..6776)
                     /gene="gatZ"
                     /locus_tag="ACHK0Z_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             complement(5514..6776)
                     /gene="gatZ"
                     /locus_tag="ACHK0Z_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGU4502829.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6817..7659)
                     /gene="gatY"
                     /locus_tag="ACHK0Z_RS14185"
                     /old_locus_tag="pgaptmp_002837"
     CDS             complement(6817..7659)
                     /gene="gatY"
                     /locus_tag="ACHK0Z_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU4502830.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGC"
     gene            complement(7969..9021)
                     /gene="fbaB"
                     /locus_tag="ACHK0Z_RS14190"
                     /old_locus_tag="pgaptmp_002838"
     CDS             complement(7969..9021)
                     /gene="fbaB"
                     /locus_tag="ACHK0Z_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU4502831.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9278..10555
                     /gene="yegT"
                     /locus_tag="ACHK0Z_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             9278..10555
                     /gene="yegT"
                     /locus_tag="ACHK0Z_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU4502832.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10552..11556
                     /gene="yegU"
                     /locus_tag="ACHK0Z_RS14200"
                     /old_locus_tag="pgaptmp_002840"
     CDS             10552..11556
                     /gene="yegU"
                     /locus_tag="ACHK0Z_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU4502833.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11553..12518
                     /gene="yegV"
                     /locus_tag="ACHK0Z_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             11553..12518
                     /gene="yegV"
                     /locus_tag="ACHK0Z_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4502834.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12492..13238)
                     /gene="yegW"
                     /locus_tag="ACHK0Z_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             complement(12492..13238)
                     /gene="yegW"
                     /locus_tag="ACHK0Z_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4502835.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYLQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13290..14108)
                     /gene="yegX"
                     /locus_tag="ACHK0Z_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             complement(13290..14108)
                     /gene="yegX"
                     /locus_tag="ACHK0Z_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU4502836.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGTVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQYSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(14173..14973)
                     /gene="thiD"
                     /locus_tag="ACHK0Z_RS14220"
                     /old_locus_tag="pgaptmp_002844"
     CDS             complement(14173..14973)
                     /gene="thiD"
                     /locus_tag="ACHK0Z_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU4502837.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14970..15758)
                     /gene="thiM"
                     /locus_tag="ACHK0Z_RS14225"
                     /old_locus_tag="pgaptmp_002845"
     CDS             complement(14970..15758)
                     /gene="thiM"
                     /locus_tag="ACHK0Z_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU4502838.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15793..15889)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15981..16253)
                     /gene="rcnR"
                     /locus_tag="ACHK0Z_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     CDS             complement(15981..16253)
                     /gene="rcnR"
                     /locus_tag="ACHK0Z_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU4502839.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16422..17198
                     /gene="rcnA"
                     /locus_tag="ACHK0Z_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             16422..17198
                     /gene="rcnA"
                     /locus_tag="ACHK0Z_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU4502840.1"
                     /translation="MPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAAT
                     ISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTAFWMFWRTWRGERNWLE
                     NMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHANDIKRRFDGREVTNWQIL
                     LFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLITVGVGAAISVQQ
                     VAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17417..17755
                     /gene="rcnB"
                     /locus_tag="ACHK0Z_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     CDS             17417..17755
                     /gene="rcnB"
                     /locus_tag="ACHK0Z_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU4502841.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17837..18871)
                     /gene="yehA"
                     /locus_tag="ACHK0Z_RS14245"
                     /old_locus_tag="pgaptmp_002849"
     CDS             complement(17837..18871)
                     /gene="yehA"
                     /locus_tag="ACHK0Z_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HGU4502842.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18885..21365)
                     /gene="yehB"
                     /locus_tag="ACHK0Z_RS14250"
                     /old_locus_tag="pgaptmp_002850"
     CDS             complement(18885..21365)
                     /gene="yehB"
                     /locus_tag="ACHK0Z_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU4502843.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGRSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21381..>22041)
                     /gene="yehC"
                     /locus_tag="ACHK0Z_RS14255"
                     /old_locus_tag="pgaptmp_002851"
     CDS             complement(21381..>22041)
                     /gene="yehC"
                     /locus_tag="ACHK0Z_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4502844.1"
                     /translation="SLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQGNRSSL
                     LQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLN
                     VLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKN
                     DSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGNYISDK
                     I"
BASE COUNT         5548 a         5609 c         5102 g         5782 t
ORIGIN      
        1 agctatcttt ttcatctcac cttcccccat taaataccaa cgacactctc tagtgtttaa
       61 atataataat ggcatgatta ttataattga ataggattat aataaatgtt ctgtacaaca
      121 tttcctacat aagtaggaat tacggacatt gaggcccttc agggtaactc catgggggct
      181 ttaatatatt tggaatagta tatttaagat gccactgttt ggttgaatat taggcatatg
      241 ctcttttttg aaatttatcg gtggcagccg ttagtattcg ctgtccccat tgcaagctcc
      301 tggtggtaac cactgaatcc tccatacttg aactgacttt ttatcctccg actttcatcc
      361 tgttctgact ccaccttttg ttttctgctc tacactatct acagaccaat cataaaggca
      421 catacgatca tggcagaatt tcccgccagc ttactgattc ttaatggcaa aagtactgac
      481 aatctaccct tgcgcgaagc aattatgctg ttgcgtgagg aaggaatgac gatccatgtg
      541 cgggtcacct gggagaaagg cgatgccgca cgatatgtag aggaggcccg gaagttgggc
      601 gtcgcaacgg tgattgccgg tggtggcgat ggcaccatta atgaagtttc tacggcgttg
      661 attcagtgtg agggggatga catacccgcg ctgggaattt tgccattagg aaccgccaat
      721 gattttgcca ccagtgtagg gattcctgag gcactggata aggcgctgaa actggcaatt
      781 gccggtaacg ccattgcgat agatatggcg caggtcaaca aacaaacctg ttttattaat
      841 atggcgacag gcggatttgg gacgcgtatt accacagaaa cgccggaaaa attaaaagcc
      901 gcgctgggtg gcgtctctta catcattcat ggcttaatgc gcatggatac tctgcaaccg
      961 gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag gtgacgccct ggtcattggt
     1021 attggtaacg ggcgtcaggc cggtggcggt cagcaattgt gtccgaacgc gttaattaac
     1081 gatggcttgc tgcaactgcg catttttacc ggcgatgaaa ttcttccggc tctcgtatca
     1141 accttaaaat ctgacgaaga taacccgaat attatcgaag gcgcttcgtc gtggtttgat
     1201 atacaagccc cacacgaaat cacttttaat cttgatggcg aaccgttgag cggacaaaac
     1261 tttcatattg aaatacttcc ggcggcgttg cgttgtcgat taccaccaga ttgtccatta
     1321 ttgcgttaat caaactattc tctcttgaac cagggagaat ccgaaatgcc ggatgcggtg
     1381 taaatacctt atccggcata cagaacaata ctacaccgta attaatttta cccgtgtctt
     1441 ctgcaatgcc agtttatcca cctctgatac agcatcatcg gtaataataa tgtcaatttt
     1501 ttcgattggc agtacctgat taaaaccgcg acggttaaat ttcgatgaat cgagaacggc
     1561 aatcacttta ttagcggcgg tgaccatcgc accgctaatc gaataacctt cattaaaggt
     1621 cgtaatacca ttaaccgcat caataccatc agcaccgaca aacattaaat cggcattaat
     1681 atcctgcagg gaacgttcag caatagatcc atgcatagat cgcgttttat ggcggaccgt
     1741 gccaccgcag acgaccagag taatgtcttt attttcggaa agggcaaacg cagccgggag
     1801 actgttggta atcaccgtga tatttttggc ggtcattaat ccttcggcga taagcattgt
     1861 ggtacttccg ctgtcgagaa taacggtcat cccttcatgg atcattttga ctgccgcctg
     1921 cgctattctg tttttcggcg cgctggcgag ctgaaatcgc tctttaaacc cgacttcctg
     1981 ggtctcggtt tcactattac cggacattat tttcgccgca ccgccatgaa agcgcgtaac
     2041 cacgcctttt tgttcgagaa agcgcaaatc ggcacggatt gtcgcttccg aggcagcaaa
     2101 tactcccgcc agatcctgaa caagcacggt tccctgttca ttcactaatt ggatgatctt
     2161 attccttcgc tcgaatgaat tcatcgtcat ttacccatat tcaattgtgg ctagtgtaaa
     2221 cgaaggggga ccattggtga atgatctgtt ttgcacaata ccgggccagc atcacgctgg
     2281 cccgcggttt cagggaatga gcaacacttt gcccggcata gcattacgag cgatgtcacg
     2341 caccgcctgg gcgaagcttt caaagcttcc acggtgagcg attaatggct ccaggcttaa
     2401 cttacgttct gtcagcaacc ggctcgccgt ttcccactcc tgccccggcc aagggctgga
     2461 gtagttcatc caactgccga taaccgtcag ctctttacgc aatattttgc caaacgttgc
     2521 cgatgttaaa tgcagatcct gatgcaacgt gcccaccagc gccagttggg catgaggacc
     2581 ggcaatctct accgccagtt cgacagtttg cggtacgcca gccgtctcga ggataagctg
     2641 attaaagcgc agttcgcgta aaacgctctg catttgcggc gcgctcattt cgctactgtt
     2701 aaatgtttgc atcgcaccga aagattttgc cagtgccagt ttttctgaac taatgtcgat
     2761 cgccgtcaca ctctttgctc ccagcgcgac agcgcactga atggccagca ggccaatggt
     2821 tccggcacca ataataataa cgtttttatt ctcacaacct tgcgctaaat gaaaagcatg
     2881 caggccaacg gtaatcggtt caataaaagc cccatcctca ataggcatat cagtaggtag
     2941 agcaaagaca tttttccgct taacgacaat atattcggca aatccaccat cacgccgtga
     3001 gccaataaaa tcatattttg cgcactggga ataaaaccct ttcaaacact ctggacaagt
     3061 aaaacagggt aataacggca cacaggcaac cgcatcgcca ggatgtaaat catcaacacc
     3121 ggatcccacc gcatcaatat agccgctaaa ttcatggcct aacgttattg gataataatg
     3181 tgcaccattt ttaaatatcc tgggtaaatc ggaaccacat aagcccgagc tggcaatttt
     3241 tacccgcacc tcatcctgat gtttaatttc aggaatgacg ctttctgcaa cgcgcacgat
     3301 accatcagta tcattcacca ctgatttcat aaaaactcct gattgttaag ggggataacc
     3361 tccccctcag gtaaaaatta ttctgcgaga acgactttct cttgtttaat aaagccacgc
     3421 gctctacgcc aggtcatgaa aataccggta agataaattg cgccgataat aatgaatccg
     3481 ggaatatttt gcggggagaa aacctgaatc agtaaccagg taatgggaga accgccctga
     3541 tccattgaag ccaccatacc cccggctttt aacgccccag cattagccgc cagttgggtg
     3601 tgcaaaccaa tagtttgcgt cgcgatccac agagtgatgc tcatgatgat gacacccgag
     3661 attaaggtgc ggaacagatt tccacgatgc acggcgactg ccatcgccac aaagaagccg
     3721 atggtggcaa gatcgccaaa cggcagcacc tgattacccg gaacacaaac agcaattaaa
     3781 atggtgagcg ggataaaaat caggcttgcc gataccaccg ccgtatgccc cagcagtaat
     3841 gcgggatcca ggccaatcag gaactcctga ccgccgaact tcgcctgtaa acgactacgg
     3901 gcctgcttag cgatgggcgt taaaccatcc atgatgggtt taatcacccg tggcattagc
     3961 agcatcactg ccgccgtttt taccgccagc tgcaatacac ctttgacatc gtaacccgcg
     4021 aggatgccga taatcagccc catcacaaaa cccacggtga caggctcgcc aaatggacca
     4081 aatttgcgct gaatatcgtc ggcgctaaat ttaattcggt taacgcctgg gattttctcg
     4141 atgatagcat cgaccagcac cgcaatcggc cccatatacg ccgacgtacc gtgcggaata
     4201 gcaatacctt ccagctcaaa gaaatttcgg gtatcgcggg caaaccagtc gccgagctta
     4261 taaacaaacg ccgcgtgaat taccacacct gccatcccta tcatccatga accggttgcc
     4321 aggtgcagca acgcgccggt gaaggtcata tgccagatat tccagatatc aacatttacc
     4381 acccgcgtca tacgggtcag taacatcgcc acgttaacca atatcgcaat cggaatcgcc
     4441 accagcgcaa tttgcgacgc ccaggtcatt ggtgaagagc ccggccagcc gacatcgacc
     4501 acatgcagat tcaggtcgaa attttccgcc atcgctttcg ccgccggacc aatggaatcc
     4561 agcattaagc caatcacaag gccaatgcca acaaagccaa tcccgatatg cagacccgct
     4621 ttaaagcaat cgcctgcctt catgcctaat attttagaaa aaataatgat gacaaccggc
     4681 agcatcactg tagggccgag gtcgagaata taacgcatga cttctgaaaa cataggtcac
     4741 ccctgtaaga tagtcagaat tttattttgt aatgcttcga taccgacacc agaaacaaaa
     4801 ggcatgccgt gaactaacgg aatatcgcca aaactgcgat cccccctggc agtggtacat
     4861 atcaaatgca caccatccat ataggtttct atttcattaa cccgacactg gattaattca
     4921 acaggaatat tatgactctg acacaactct ttaatttctt ccgccgccat cgtagaggtc
     4981 gcaaccgcgc ctccgcaagc gacaataatc ttgcgtttca taaatagtcc tttttattgt
     5041 tacagaggga tttattcgtc tgaatctaaa acatactttg tgaagtattc ctttaactgg
     5101 gtttcaggaa gagtgattag tgtctcgacg atatcgggct gttgtaactt gccaaataaa
     5161 cagcgtaaaa gtttcaattg ctgctgcgga ttttccacaa tcaacgcaat aaccaacgat
     5221 accgccacgt cgttatcatc atccgcttgc tgaaaatgaa ctttatttgt tggccttaac
     5281 agataaatgg ctgacgactt agcatgaatc gcctcacaat gcggtattgc aatggcgtgc
     5341 tgctcaagca ttatcccggt agggaattct gcttctctgg caattaatgc ctgtggccag
     5401 gtatcatgaa ccacaccttt ggcgagcatc tcattaccga tatgggttaa aacttcgcta
     5461 cgatcgacaa aagaaattcc gctacgaaca aacaggttag tcatataccg tccttattcc
     5521 gcacagccgt agcgataggc gcgcaaaaca tcataaattt tatccataat cagctgatgc
     5581 ggtattgctg ataattcccc ggactgaatg cgttcaaatt gtttgggaag atactgacta
     5641 atcatgccca gtgggatgtc cacgccttca agattcacca tcatcgtttc gacgctattt
     5701 ttaatccgac tatgcggcca gtaataacga atacgatccg acaggctgta acgaatatcc
     5761 agtaatgaat cgttaaaacc cgtacgataa tattttttcc agtattgcgg ttcgtcgagc
     5821 atcacttctt caattaccgc caggcaaccg ctgcgatttt caggggcgat aagttcctgc
     5881 tcaatttgtg ccagtgcaaa tatcgcctcg cgtaaagcaa aggttaatgc gggaccgact
     5941 ttcaatattg caaagtgatc gcggactaat tcccaataag ccgtccgggt ctggtaatcg
     6001 gtagaatgtg cttcataaac cattcgggtg ttttctatcc attgcgccag cggctgcgct
     6061 tcctgcggct gataatggat aatattgctg tgatcaaatt ccacgcccgg ctgcaccacg
     6121 atggcaatca cgcgtgttaa cgcctctgtc agcccacggg caataaaggc cttttgatgc
     6181 gtacgtaaag tattggcggt atcttcaaca tgggtgatgt gtactgactg aatggcgctg
     6241 gcctcaccgc ccggaaccgg aacttcggtg ccaatgacat agctcagttg ctcacgctgg
     6301 caatctgtcg ccacactttc cgcagcaaag caaagcacag cagctcgttc cgcaaccgtt
     6361 tctggtgcta acggtatggg atcccccgcg caggacattg acgcatcaag atgaatttta
     6421 ctgaagccgg cacgaacata ttcctttacc agctcgacgg atttttccat cgccgcatcc
     6481 gcattttctt gctgccagca gtttggcccc agatgatcgc cgccgagaat aatgcgttcg
     6541 cgtgcaaacc caactttatc ggcaatcgta aaaacaaatt cgcgaaagtc tgccggtgtc
     6601 attccggtat aaccgccaaa ttgattgacc tggtttgacg ttgcttcaat cagcactttg
     6661 cgcgtgctgt tgcgatcaaa tgccagcgcc gcttcgataa ccaacggatg ggcagaacag
     6721 actgaacata tgccgatatg ttcaccagct ttatgccggg caattaacgt tttcatgttt
     6781 tttccttatt aaatggcagg tgcgttatgc cctgccctag cagccacaat cggcaatcac
     6841 tttgctcacc acatcgcgca ttgcggattt agccgactgc aaataatccc ggggatcggt
     6901 cgcttcaggg tgctcggtca ggtaattttt taacgcctgc gagaaggcat ttttcagctc
     6961 cgttgcaacg ttgattttgc atatccccag tttgatggtt tgctgaatat ccttagtcga
     7021 taaccctgac gcgccatgca gcaccagcgg taagttcacc cactggcgaa tgttctccag
     7081 tcgagaaaaa tcaagcgccg gtgcgctggc atacatccca tgagccgtgc cgatggcgac
     7141 cgccagggaa tcaattccgg ttgcctcggc aaattcacgc gcctgagcgg ggttggtata
     7201 aaaagcatcg gcttcattga cttgcacatc atcttcctgg ccgccaagtt gccccagctc
     7261 cgcttcgacg ctgacatcaa agcgatggca aaaatccacc acctctttga cccgtgaaat
     7321 attttgcgca aaaggcaaat gcgaggcgtc aatcatgact gagcgcacgc cagaacgaac
     7381 cttctgagcg atatcgtcaa atttcgtgtg atggtcgaga tgaattgcca gtggatggtg
     7441 atattgcttc gccatcgcgc tgaccagcgc caacagattt tctgtaccag catgagtaaa
     7501 tgtgccaggc gttccggcga tgatgaccgg cgcatgcagg ttggcagcgg tttccaccac
     7561 cacttgcatc gtttcgagat tgtgaatatt gaatgccgga accgcataac cgccgcgctg
     7621 tgcgttgttc agcatctgct ttgtcgatac cacgtacatt ttcatatcct gtcgtttgtt
     7681 ttcgatttca aaatataatg aaatgatttg tttttaaatg tcgagataac gatcacaaaa
     7741 acgacaatgt gaaaattgtt cgaggagtaa aaggcaaaaa aaaacggcct cccgataggg
     7801 aagccgtagc aaagtgcgcg tgtttttatg ccggatgcgg tgtaaacata cgtggcagtg
     7861 ctatgtaggc atgataagac gcttccgcgt cgcatcaggc ataggttgcc ggatgcagtg
     7921 taaacgcctt atccgtccta cggggtggtg ctgttcgtat gaggcgtatc aggcaatagt
     7981 aattttgcta tcgagataaa cgtcctgcac ggcattaatc agtttcacgc cgtcagccat
     8041 cgatttcttg aacgccttac gtccaagaat cagccccatt ccgccagcgc gtttgttgat
     8101 aaccgcagta cgcactgcat cactgaggtc agtttcaccg cctgcagcac cgccggagtt
     8161 aatcagccct gcgcgcccca tatagcagtt agctaactga taacgcacca gatcaatcgg
     8221 gttttcgctg gttaacttgc tgtacacgcg atcgtcggta taaccgtaat taattgcttt
     8281 atagccgccg ttattttccg ccattttttg tttgacgata tctgcaccga tggttgccgc
     8341 cagatggttt gcctgaccgg tcaggtcggc ggaaacatgg taatcaacgc catctttctt
     8401 aaaggcggag ttacgcaaat aggcccacag cactgtcacc atacccagct cgtgcgcacg
     8461 ttcaaaagcc gcagaaattt cttcaatctg gcgacgtgac tcttccgagc caaaatagat
     8521 agtcgcacca accgcaaccg cgcccatgtt gaacgcctgc tccacgctgg catacagcgt
     8581 ttgatcgtag gtattcgggt aacttagcgt ctcgttgtga ttaagtttga cgaggaatgg
     8641 aatgcgatgc gcataacgcc gcgataccga cgccagcacg ccgtaagttg acgccacaca
     8701 gttacagccc gcttcgatcg ccagttcaac aatgtttttc gggtcaaagt agagcgggtt
     8761 agcagcaaat gaagctccgg cagagtgctc aacgccctgg tcaaccggca gaatagaaag
     8821 atatcctgtg ccagccagac gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc
     8881 tggcgggcga ttattgtcaa tcattacgcg gtctacgtag tcatgtccgg ggagataaag
     8941 ctggtcagaa ggaattgtca tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag
     9001 caactgcgca atatctgtca ttactatgct cccgtaaatt ccgattggat atcggctatg
     9061 gattgtcctg gcccgccttt tgcgggcaat cataatcctg gtcgttacgg gcgagttttt
     9121 ccatcttttg gatgtttttt cagcgttttc tgttggctcg attcatcaga aaaaatgtta
     9181 gcgcggtcaa gttttcaccg caaaggtatt taaaaggtat tattaagtgg tattatcatc
     9241 gcgtacctta cattacctgt catgaaggaa ttaaaagatg aaaacaacag caaagctgtc
     9301 gttcatgatg tttgttgaat ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg
     9361 gttaagtaaa agcggtttta gtgccggaga aattggctgg tcgtatgcct gtaccgccat
     9421 tgcggcgatc ctgtcgccaa ttctggttgg ctccatcact gaccgctttt tctcggcgca
     9481 aaaagtgctg gcggtattga tgttcgcagg cgcgctgctg atgtatttcg ctgcgcaaca
     9541 gaccactttt gccgggttct ttccgttact gctggcttac tctctaacct atatgccgac
     9601 cattgcgctg actaacagca tcgcttttgc caacgtgccg gatgtggagc gtgatttccc
     9661 gcgcattcgt gtgatgggca ctatcggctg gattgcctcc ggtctggcat gtggtttctt
     9721 gccgcaaata ctggggtatg ccgatatctc accgactaac atcccgctgc tgattaccgc
     9781 cggaagttct gctctgctcg gtgtgtttgc gtttttcctg cccgacacgc caccaaaaag
     9841 caccggcaaa atggatatta aagtcatgct cggcctggat gcgctgatcc tgctgcgcga
     9901 taaaaacttc ctcgtctttt tcttctgttc attcctgttt gcgatgccac tggcgttcta
     9961 ttacatcttt gccaacggtt atctgaccga agtcggcatg aaaaatgcca ccggctggat
    10021 gacgctcggc cagttctctg aaatcttctt tatgctggca ttgccgtttt tcactaaacg
    10081 ctttggtatc aaaaaggtat tattgcttgg tctggtcacc gctgcgatcc gctatggctt
    10141 ctttatttac ggtagtgcgg atgaatattt cacctacgcg ttactgttcc tcggtatttt
    10201 gcttcacggc gtaagttacg atttttacta cgttaccgct tacatctatg tcgataaaaa
    10261 agcccccgtg catatgcgta ccgccgcgca ggggctgatc acgctctgct gccagggctt
    10321 cggcagtttg ctcggctatc gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatcc
    10381 ggaaccggta aacgggctga ctttcaactg gtccgggatg tggactttcg gcgcggtgat
    10441 gattgccatt atcgccgtgc tgttcatgat ttttttccgc gaatccgaca acgaaattac
    10501 ggctatcaag gtcgatgatc gcgatattgc gttgacacaa ggggaagtta aatgaaaaca
    10561 gaacgtattc tcggtgctct ttatgggcag gcgttagggg atgcgatggg gatgccctcc
    10621 gagctttggc cacgcagccg cgttaaagca cactttggct ggattgaccg ttttctgcct
    10681 ggaccaaagg agaataacgc ggcctgttat tttaaccgcg ccgaattcac cgacgatacc
    10741 tcgatggcgc tgtgtctggc ggatgcgtta ctggaacgtg aaggcaagat cgatccggat
    10801 ctgattgggc gtaatattct cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta
    10861 ctaggtccga cctcgaagat tgcgcttaac gccattcgcg acggtaaacc cgttgctgaa
    10921 ctggaaaaca acggcgtgac caacggcgca gcgatgcgcg tctcgccatt aggttgtttg
    10981 cttccggcgc gtgatgttga ttcctttatt gatgatgtgg cgctggcgtc gagcccgacc
    11041 cataaatccg atctggcagt tgcaggtgcg gtagtcatcg catgggcgat ttctcgtgcc
    11101 attgacggag aaagctggtc agcgattgtt gattccctgc cttcaattgc gcgacatgca
    11161 cagcaaaaac gcatcactac cttcagcgcc tcactggcag cacgtctgga gattgcgctg
    11221 aaaattgtgc gcaatgccga cggcaccgaa tccgccagcg aacagcttta ccaggtcgtt
    11281 ggcgcaggta ccagcactat tgagtccgtt ccgtgcgcca ttgcgctggt tgaactggca
    11341 caaaccgacc cgaatcgctg cgccgtcctg tgcgctaacc ttggcggcga cacagacacc
    11401 atcggcgcta tggcgacggc gatttgcggc gcgttgcatg gcgttaacgc tatcgatcct
    11461 gcattaaagg cggaactgga tgcggtaaat cagcttgatt tcaaccgcta tgccacagcg
    11521 ctggcgaaat atcgtcaaca acgggaggcg gtatgagcgg cgctcgatta cacacgctgc
    11581 tgcctgaatt aacctcgcgt cagccggtga tggtcgtcgg cgcggcggtc attgatgtga
    11641 tcgccgacgc ttatgccctc ccctggcgtg ggtgcgatat cgaactgaag cagcagagcg
    11701 ttaacgttgg cggctgcgca ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag
    11761 cgggtaatgc cttgccgctc ggtcagggcg tgtgggcgga gattattcgc aaccggatgg
    11821 cgaaagaggg gttaatcagc ctgatcgata acgccgaagg tgataacggc tggtgtctgg
    11881 cgctggttga gccggatggc gaacgcactt ttatgtcatt cagcggtgtg gaaaatcagt
    11941 ggaatcgcca gtggctggcg cgattaaccg ttgcgcctgg cagcctgctc tatttttccg
    12001 gttatcaact ggcctcgccc tgcggcgaat tgttagtgga atggctggaa gagctgcaag
    12061 acgtgacgcc gtttatcgat tttggcccac gtattggcga tatcccggat gcattactgg
    12121 cgcggatcat ggcctgtcga cctttagtgt cgctcaatcg tcaagaggct gagattgccg
    12181 ccgaacgttt tgctttatcc gcagagataa caacacttgg cgagcaatgg caggagaaat
    12241 ttgccgcgcc gttgatcgtt cgtctcgata aagaaggcgc atggtatttc agcaacgacg
    12301 cttctggctg cattccggca tttccaacgc aagttgtaga caccattggg gcgggcgaca
    12361 gtcatgcagg tggcgtactt gccgggctgg cctctggtct gccactggcg gatgccgtat
    12421 tactgggcaa tgcagtggcg tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa
    12481 cgcgcgagga actactcctc gcacacaaaa acgtatagat cgctgcgaca gtggctaatg
    12541 ctgtactcaa taggccgttg ctgttggtca agcgccactt gcttgatcac cagcaccggt
    12601 attttgctat ctaactgaat atgtgactga aactcggcat ccggcatccg ggcgctaacg
    12661 cgggaacgcg tacgttgtag gtaaatgtgc tggctgcgga aatagtcata aagtgaaatc
    12721 ccgatggcat caacatcatg aattaaatgc gccggaaccc acgattcctc aatcgatacc
    12781 gcttcttcat cgacatagcg aatacgctta agcaaaaaga catcacttcc tgcttcgacc
    12841 gccagctgct gcgcgacttc ttcgggacat ttcacgacac gcttgttaac ccatagcgta
    12901 tcgggctttt tcccgcgcaa taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag
    12961 tattcgaaga tgttgttgat ctgcgtgccg taaccgcgcg aacgcgtcac cacaccctct
    13021 tcttccagcg cctgcatcgc cttgcgcacc gtaatgcgcg aaacgccggt taactgactt
    13081 aaatcacgct caccgggcaa aatattgcca tgctccagca ccccgctgcg cacggcattt
    13141 tttaccgttt cggcaaactt aatataaagc ggcgtgttat ccgccgctaa aatacgctcg
    13201 ttcaactgag cgataagctg ggtatgcgct tgttccattt gtctttttcc tgccggggag
    13261 tctggtggga gtatatcact gcttcttatt taaggcgttt ctttaatccc atcaacgaat
    13321 ccctgcaact cctccaccgt gccattaaac acattaaaat ccaccggacc attgatgcca
    13381 tctacctgtc cacggtcgga atactgccag aagcgccagg ccattccgtc attgtccgga
    13441 cgacgttgat aatagtgagc cacccaccac ggatattcat taaaatagcc cgccagattg
    13501 gtgtgataaa aaacggttcc tgagtaaata atcggctttt ttcccgtact tttttcgacc
    13561 atttttagcc actgacttac tcgtttgcgt aattcttttg ccgataattt tccccgttct
    13621 tctacgtcca gtacagcagg gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat
    13681 cgtgcctgaa ccggcgctgc taccgacggg gagaaataat gatacgcccc gcgcagcagg
    13741 ccattttcgc ggcttagttg ccagttacgc gaaaaatacg ggtccaccag cttttcgcct
    13801 tccgtcgcct taataaaagc aaactgtaag cggataccgt tgtcgcgcat ttttgccacc
    13861 cgctgccagt cgatccgctc ctgccagcga gaaacatcta ttccgtgaat ggtgtaactg
    13921 gcaggaatgc gaatagcgaa agatttaacc gggcgataac cgtagaaatg gatgtagtct
    13981 gtaatctgaa ctgctgtcga atagaaaaaa ttcaccgtct gacgcggata aattgcaacg
    14041 atggaaatta gcccaagagc gcataataga gaggtgagtt tttttcgact ggtaattctt
    14101 agctgcattg gtttcatccc tgaatgtcag tgccaaaggc tgacaataac caaagcagct
    14161 atagtacggt gctcaccacc aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac
    14221 ttccagcgtg tcggcctggg ctaatgctga tgaaagccag ctttttgcct cctgtacggt
    14281 gtcagcccag tttgtatggc gcgggcgtag tgcagccaac gccgcagaga gcgtacaacc
    14341 agtgccgtgg gtgtttttgg tcataatgcg cggtgcggta aaccgttgtt caccctcgcg
    14401 ggtaaacagc cagtccgggc tttgctcatc atccagatga ccacctttca ttagcactgc
    14461 gccacagccc atcgccaaca gcgatcgccc ttgttccagc atttcctgtt cggtgcgcgc
    14521 gtgtggcgcg tcgagcaagg cggcagcttc gggcaagttt ggcgttatta atgaaacctg
    14581 tggcaataat cgactgcgca gcgtagcaac cgccgaaggt gaaagcagcg ggtcgccgct
    14641 ttttgccagc ataacggtgt cgagtaccac gttttggatc tgataacgtt gcaaccgttc
    14701 tgccaccgct tcaacaatat cggtttccgc caacataccg attttagtgg tgtcgattcg
    14761 cacatcgctg aacaccgaat cgagctgggc ggcgacaaaa tcaggctcaa tgcgatacac
    14821 cgactgtacg ccacgggtat tttgcgccac cagtgcagta ataactgagc aaccataagc
    14881 gccaagtgcc gagaaggttt taagatcggc ctgaatcccc gcaccaccac tcggatcagt
    14941 accggcaatc gtcagagcgt taattcgttt catgcctgca cctcctgcgt caattgccag
    15001 agcgcatcaa ggaaatgtgg aacaaaactg cctggcccct cgcttctggc gactgcgcgt
    15061 tctccggctt gtttcatcca gtgacaggca gatgcgacat tttccagcgt atcgcctggt
    15121 aacgcacagc aggcagcgac aaccgccgat aatgcacagc cagttcctac cactttggtc
    15181 attaacggat cgccaccgtg aatgccaacg gcgcgatggc catcggtaac ataatccacc
    15241 tcgccagtga ccacgacgat tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc
    15301 gcgttagctg cggcgtcagt ggtatccact ccccgtccac cattagcaat gccagctaat
    15361 gccatgattt ccgaagcatt accacgtatc gctgccggtt taaaagataa aagttcatga
    15421 caaaaatggc ggcgataatc gagcgcacca accgctactg gatcaagcgt ccagggtgtt
    15481 tgagagcttt ttgcttgctc aacggcagca cgcatcgcct gagcgcgtgg ctgcgtcagt
    15541 gtgccaacgt taatcaacaa ggcactggcg atagccgcaa actgactggc ctcttcggtt
    15601 tcgataacca tcgctggcga tgcaccgagc gccagcaagg tattggcagt aaaggtttgc
    15661 accacatcgt tggtcatgca gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgcg
    15721 tgcgcagatt gcgctgaact cagcaggtcg acttgcatag tttgctcctg ccataacgtg
    15781 aagaagcaat gacctggtgg tccgtgactt ccctacgctg gcattatcca gatcaggtga
    15841 tacgggtatt tctcagcctt cacgcagaag ggcaccccga gtcgtttggt tgcgatgata
    15901 aatcgcagag gaggatggta atgtccagcg cacgcgttgt aaacgaggaa aaacggcagt
    15961 tttacaatcg cgttaataaa ttatttgata tatgaatcca gcaccttcag aacgacatcc
    16021 agatcttctt cacgttttag ctcatccccc tggtgaacga tgtgttccgt cagatgacct
    16081 ttaatcactt cccgcatcag accgtttacc gcgccacgga tagcagcaat ctgttgtaaa
    16141 actgcagcgc attcgtgcgg ctcgtcgagc attttcttga gcgccacgac ctggccctga
    16201 atcttactgg cacgcgcttt cagtttctgt ttatcacgga ttgtatgaga catggcaaca
    16261 cctggttaac aagaatatga aaaatcatag cactattaat ctactggggg gtagtatcag
    16321 gtactggggg ggagtagaat cagattgccg aattaatact aagaattatt atcatgaccg
    16381 aatttacaac tcttcttcag caaggaaacg cctagttctt catccccagc gccatcttac
    16441 ttggtgcgct tcatggcctg gaaccagggc actcaaaaac gatgatggcg gcgtttatca
    16501 tcgccatcaa aggcaccatt aaacaagcgg tgatgctcgg actggcagca actatttcgc
    16561 ataccgcagt ggtctggtta attgcctttg gcgggatggt gatcagcaag cgctttactg
    16621 ctcaatcagc agaaccgtgg ctccagctga tttccgcagt gatcattatt ggcaccgcgt
    16681 tctggatgtt ctggcgtacc tggcgcggcg aacgcaactg gctggagaat atgcacgagt
    16741 atgatcatga acatcatcac cacgatcacg aagatcacca cgaccatgga catcatcacc
    16801 atcacgaaca tggcgagtat caggatgccc atgcacgagc ccatgccaat gacattaaac
    16861 gacgctttga tggtagagag gtcaccaact ggcaaatttt gttatttggc ttaaccggtg
    16921 gtcttatccc ctgtccggca gcaattaccg tgctgttgat atgcattcag ttgaaagccc
    16981 tgacactggg cgcaacactg gtcgtcagtt tcagcattgg cctggcgtta acgcttatca
    17041 ccgtaggcgt tggcgcagca atcagcgttc agcaggtcgc aaaacgctgg agcggattta
    17101 acactctcgc taaacgcgcc ccctattttt ccagtctgtt gattggctta gtcggtgtgt
    17161 atatgggcgt acatggcttc atgggcataa tgcgataact caatccttta ggcttgcatg
    17221 ttatcttcag cccaggatga ttcttaaatc agctatttcc gctgactctc tgctcgctaa
    17281 ggatgctttt agggcatcct ttcatttaca ctttttacga aatcatggga tcactaacaa
    17341 aatatcgctt gtcagttata ttgtatggca ggaaagatat gcgactgata ttacagatcc
    17401 ccaaagtgga gagtttatga ccattaaaaa taagatgttg ctgggtgcgc ttttgctggt
    17461 taccagtgcc gcctgggccg caccagccac cgcgggttcg accaatacct cgggaatttc
    17521 taagtatgag ttaagcagtt tcattgctga ctttaagcat ttcaaaccag gcgataccgt
    17581 cccagagatg taccgcaccg atgagtacaa cattaagcag tggcagttac gtaacctgcc
    17641 cgcgcctgat gccgggacgc actggaccta tatgggtggc gcgtacgtgt tgatcagcga
    17701 caccgacggt aaaatcatta aagcctacga cggtgagatt ttttatcatc gctaaaaaaa
    17761 gccccctcat catgaggggg aaatgcagac accttgttat tttttattat tagccacttg
    17821 ctcgtcttgc ttgttattag tcgtatttca cgttgattaa tgcggttgcc tccagtgcgc
    17881 cagatttaac tttgtttgta tcgtagacgt agtaactggc agttatcgga attgaaaccg
    17941 ttgtatctgt aagttttgac tttgccgcaa tttcttttaa atttgtgtat cctttactgc
    18001 tgttagtgat atttaaaagt gtgactgacg aaccattact ttcaagaata aatcctacgc
    18061 cattatcttc cgcacctttc agtattgtgt tgctgttggt taacaagtca gaacttgata
    18121 aatacacctg cattttccgt gatgtttgat tttgttgacc taataaattt ttacaacgca
    18181 gaataatatc cccttgttta ctatttattg ttgctttgtt acctgtggct ggtaattgtg
    18241 aaacgggaat actgtctaaa gtaattaata aatcgtccgg tttgcaggtt gtggttatcg
    18301 ggttgtatga caatgtcaga ttaaaggaat agacacctgc attttgtgcc aatgcatttc
    18361 tgtagctatt tacacatgca tcccatgact tgccacctaa taaacacagt agaaattgta
    18421 ctgcggcact cccgcgtgtc gatgatgaag cactggataa tgtcgcaact ccattaatca
    18481 tcactgaacc gccgcccccc gcagaaacat tcacttttgc attactggcc gcaggtgtca
    18541 ccgttaccga ataaccactg gtgaaactgg tctgattatt tgataacgtg atgttgtttt
    18601 tattggtcca ttcaaaatgt aaatttaatt taatggtatc gttataaagt ccaacaataa
    18661 tatccgacgg gctcataggg gtgatagtat ttgtactcac gcatgacgaa tcattaaaat
    18721 tatgctcata tgtcgccatt tgtgtttgag taagtgcaaa atccccctga acaaccaaat
    18781 tgtgctgaac agataactca ctataacatg ccgcatagct cacaggcata accatcatca
    18841 ttactataaa taacattata cgaatattca taaccacctg actattactg gcaaatatag
    18901 tttctacttt catcaatctc tttaccgaag gtgattgtgc atgaaagtcc ttgttgctta
    18961 tcaattgcta cattaaccga tggcggtact tcattggtgc gaataaataa ctggcttccc
    19021 tgaccgacaa cgccaatatt atgaccatgg atatcattga cttcataacc aaacattaat
    19081 ggttgcccat ccgctcttaa cgcttttata aaccagggct tgcgctgatc ggtatcaaaa
    19141 ttaaccagta caaccgcgcc gcgataaggg gcggcaattt tccggttgcc acgtaattct
    19201 gcttcgctat cgctttgcga cacatccagc atcaggtgat tttcccgata aggtgtcatt
    19261 ccgtcgtata ccaccactcc attacggttt gttgtgcgat atttttgccc attgacataa
    19321 gcatctttaa ttcctggcgc attcatcaca gcaaacgttt cggaaagacg gttcgccaga
    19381 ttaacgcccc ccgaccaggc gacaatgccc cctgaaacac tggctccagc ctgtcgataa
    19441 gtactcgact gactataact gccattcact gtcgcaaccg gcgcgttcca ggtcaaattc
    19501 gccccagctg tcgtttcatt tccctgattc tgataactca ggttgacccc atagttaaac
    19561 tgatcccggc ttccaacggt tcctgataat cccgtattat ttgaggcaaa cccctgatca
    19621 tcaaacgtcg ttgagttaga catatatatt tgccgacgag gcgtcgtaac gtcatcaccc
    19681 caatcaaagg gaatcgatat aaaaatatta aaacgtttct cttcatgatg attctcgtca
    19741 tacgcctggc ttgccgcgag gatatagctt atccgccgcc agttattgga ataactcaac
    19801 tgataatcct tactgctgcc gctacgcccc cagtaatctc gccataacgt acttaaggac
    19861 acagaccccc agccttctgg caatgactgg ctcatattgg cagagaagct atttttacgg
    19921 ccaaaatcgt tctggtaata atcggcaatg tcatagacat cgttttcatc acggcgataa
    19981 ttatctttat tgtttgccca aacgtgatca ttaaatgtcc ggtaatcacg cgatgaataa
    20041 cgccaggccg ccagaccaaa acgcgtcgac gtttggctca caaatttgtt gtaggcaatt
    20101 tgataacttt gcccgtcaaa cacatcgccg ttgtcttgtt tactatgcga cttcgtggca
    20161 tcgacggaaa tggcaccaaa gcgtgtattc cagccagtcc ccaaagtaaa cgcgtaataa
    20221 ttattcgcga ccatcgagcc accatacagc gtcaataaat tattaaaacc atactgataa
    20281 cccgcctgga caaaatcact ttgtttgctc gccccttcaa tatggctacg acccgccgca
    20341 aaatcatatt tcgatacgcc gggttgcagc atatttggca ctgctgcata aggcaccaga
    20401 taggtggtta ccgagccgtc cgcctctttc acgctgacat caagatctgc tccaccaccg
    20461 gccaactgca aatctgtaat cgcgaacggg ccaggaggaa cctctttctg ataaaccaca
    20521 aaaccgttct gttcaatagt taccagcgca ttactctgag caatcccctg cacccgtggc
    20581 gtaaaatttt gtttcgagtt aggcaacatc tgcatatcac gaaacaaccg cacaccgctg
    20641 aagcgaacag aatcaaaaat atcgcttgat gtgtacatat cacccacgcg aagcgtgccg
    20701 agaagttggg caaatccacg ttccagatac agggtattgc ttttccacac ccctggattg
    20761 ttatttgttt tactgaaact ggcatcagaa tgcagttgcc accccagtaa atttaacccg
    20821 ctgttaaaac gtacatatgt actcttacta ttacccgacg ctttatagtc actgtaatac
    20881 tgactcacat aataagaggt ataaaacgca ttaatacccc gctcccagtt ttccggcgga
    20941 acatagccac tttccagttc ttccacccag gcctgcggga cactgaaatc gagacgaaaa
    21001 accccgatat cccaggtata gctcctaccc tgaacaagtt gctcaaatgt taaacattgc
    21061 ttaccgctgg cgaagttatc gctattaatg cctaaccgct tgataacttc tattgataaa
    21121 catgtttctt gcgggttgtc tttaacaata atctcatatt tcccgcgcca ttgcttattg
    21181 acataaatat cgatgtcata ctgccccggt aagggttgat tatcatcaag acgaatattt
    21241 gcaacctgct ggtctttcat tccacctatc ataaaatggg tatcaaaggt ttcttcagct
    21301 gcataagctt caatgccgag caataacgct acgattgctg atgcaagtgg ggtcattctc
    21361 aacataggca gctcctgcaa ttaaattttg tcactaatat agttgccgtg gtcatcgata
    21421 atggtcagat accagttatt tgcattatta cttttgacat taacactctg actttctaag
    21481 ggggcaatca taatagtttc ataattgact ttaacattat tagctttgac atccgaaatc
    21541 gtcacccaat tagctgagtc attttttatc accaatccat tgccactgcg atttaccagt
    21601 aactttttaa aggtcgcttt atttaccgga gcaatacccg ctggccggta aaacaactta
    21661 attctgtttt gcatcgcaaa cttcagtgca tttttgcctt cttgctctgg actatttggt
    21721 ggaatatcca gaacattcag ataaaaaatg ctttctttat tagtgggcag tttattcggc
    21781 ataattttga tttttacttg ctgcccggaa tttgccccta tttttgccac tggtggcgtt
    21841 aacatgaaag gaacctgaat tttttctggt ggtaacgacg tatcgccatc atcaatccac
    21901 gcctgcagca gcgaagaacg gtttccctgg ttcatcaact gcaccataac ttctttattt
    21961 tctgccgggt aaataatgcg cgtcccgtag ataacaattc cggcatgtgc aggaaggacc
    22021 atagaaaaaa tgagtaaaga t
//
LOCUS       DAZFWP020000079        21512 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFWP020000079 DAZFWP020000000
VERSION     DAZFWP020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.079"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..543
                     /locus_tag="ACHK0Z_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     CDS             19..543
                     /locus_tag="ACHK0Z_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HGU4502845.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            627..1313
                     /gene="lpfB"
                     /locus_tag="ACHK0Z_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     CDS             627..1313
                     /gene="lpfB"
                     /locus_tag="ACHK0Z_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU4502846.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALITTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNSNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     TGEMLTKPIN"
     gene            1337..3868
                     /gene="lpfC"
                     /locus_tag="ACHK0Z_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     CDS             1337..3868
                     /gene="lpfC"
                     /locus_tag="ACHK0Z_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HGU4502847.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3879..4934
                     /gene="lpfD"
                     /locus_tag="ACHK0Z_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     CDS             3879..4934
                     /gene="lpfD"
                     /locus_tag="ACHK0Z_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HGU4502848.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4940..5464
                     /gene="lpfE"
                     /locus_tag="ACHK0Z_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     CDS             4940..5464
                     /gene="lpfE"
                     /locus_tag="ACHK0Z_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU4502849.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5717..7408
                     /gene="eptB"
                     /locus_tag="ACHK0Z_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             5717..7408
                     /gene="eptB"
                     /locus_tag="ACHK0Z_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU4502850.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7500..7576
                     /locus_tag="ACHK0Z_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     tRNA            7500..7576
                     /locus_tag="ACHK0Z_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7534..7536,aa:Pro,seq:cgg)
     gene            7611..7744
                     /locus_tag="ACHK0Z_RS14295"
                     /old_locus_tag="pgaptmp_002859"
     ncRNA           7611..7744
                     /ncRNA_class="other"
                     /locus_tag="ACHK0Z_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8490..10097
                     /gene="dppA"
                     /locus_tag="ACHK0Z_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             8490..10097
                     /gene="dppA"
                     /locus_tag="ACHK0Z_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU4502851.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10248..11267
                     /gene="dppB"
                     /locus_tag="ACHK0Z_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             10248..11267
                     /gene="dppB"
                     /locus_tag="ACHK0Z_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU4502852.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11277..12179
                     /gene="dppC"
                     /locus_tag="ACHK0Z_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             11277..12179
                     /gene="dppC"
                     /locus_tag="ACHK0Z_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU4502853.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12190..13173
                     /gene="dppD"
                     /locus_tag="ACHK0Z_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             12190..13173
                     /gene="dppD"
                     /locus_tag="ACHK0Z_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU4502854.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13170..14174
                     /gene="dppF"
                     /locus_tag="ACHK0Z_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             13170..14174
                     /gene="dppF"
                     /locus_tag="ACHK0Z_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU4502855.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGISFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14204..15475)
                     /locus_tag="ACHK0Z_RS14325"
                     /old_locus_tag="pgaptmp_002865"
     CDS             complement(14204..15475)
                     /locus_tag="ACHK0Z_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4502856.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15951..16058
                     /locus_tag="ACHK0Z_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             15951..16058
                     /locus_tag="ACHK0Z_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4502857.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16434..16541
                     /locus_tag="ACHK0Z_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             16434..16541
                     /locus_tag="ACHK0Z_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4502858.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16917..17024
                     /locus_tag="ACHK0Z_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             16917..17024
                     /locus_tag="ACHK0Z_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4502859.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17400..17507
                     /locus_tag="ACHK0Z_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             17400..17507
                     /locus_tag="ACHK0Z_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4502860.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17594..19273)
                     /gene="bcsG"
                     /locus_tag="ACHK0Z_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             complement(17594..19273)
                     /gene="bcsG"
                     /locus_tag="ACHK0Z_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU4502861.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19270..19461)
                     /gene="bcsF"
                     /locus_tag="ACHK0Z_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             complement(19270..19461)
                     /gene="bcsF"
                     /locus_tag="ACHK0Z_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU4502862.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19458..21029)
                     /gene="bcsE"
                     /locus_tag="ACHK0Z_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             complement(19458..21029)
                     /gene="bcsE"
                     /locus_tag="ACHK0Z_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU4502863.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            21302..21490
                     /gene="bcsR"
                     /locus_tag="ACHK0Z_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     CDS             21302..21490
                     /gene="bcsR"
                     /locus_tag="ACHK0Z_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU4502864.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5477 a         5213 c         5652 g         5170 t
ORIGIN      
        1 atataaatgg agttataaat gaaaaaggtt ctgtttgcct tatctgcgct tgcgctgacc
       61 tctacttccg tattcgcagc tgatgcgggc gacggtactg ttaaattcac cggtgagatt
      121 gttgacgccg cttgtgttgt gtctccagac actcaaaaac aagaagttgt tctgggtcag
      181 gtgaacaagt ctgtatttac tgctatgggt gataaatcca cagcaacgcc gttcaaaatc
      241 aaactggaaa actgcgatat ctccactttc aaaaatgtgg aaatcagctt taacggcgtt
      301 ggcgatgcag acaacagcaa actggtttct gtaagcactg aaccaggtgc cgcaactggc
      361 gtaggtatcg gtatttatga taacaccaat accctggttg acctgaatac tggtaaatct
      421 gctactgtcc tgaaagaagg tcagaccgtt ctgtatttca ccgctaacta tgtctctacc
      481 aaagatgcag taaccacggg ttacggtaac gctgaagtcg acttcaacct gacttacaac
      541 taatcgatcc tgacggaacg atcatctctc aatggcaaca gagactgtgt tgccattatt
      601 cacaccaatg actcagggac cgaatcatga aacgtattag ctttgctgtg ctggcacttt
      661 taatgaccgc acaacagtgt tttgctggtg gcgtagcctt aatcacgacg cgtgttattt
      721 atgatggcgg caagaaagaa gcgtcgctga ccgttgaaaa ccataataaa aatgaagagt
      781 ttttgatcca gtcatggatt gatgatgtga atagcaataa aaaaacaccg tttattatta
      841 caccgccatt attcaaactt gatcctgata aaaataacgt cttacgtatc gtcaatattg
      901 aagatacctt acctaaagat cgtgaatccg tttactggat taatgtaaag gccattcctg
      961 cgaaaagtgc tgaaaatgaa aataaaaacg tcttacagat agccgttcgc acccgtttga
     1021 agttgttcta tcgtccaaaa ggtttgccgg gaagcaacct cgaggccatg aaacagttac
     1081 gtttccagcg tcaaaataac caaattgtgg ttaataacga ctccgcttat aacctgacct
     1141 ttaatcagtt ttccattaat ggacaaaaga ttgaacgggc gggaatggtt ctcgcgaaag
     1201 gaaagttaaa cattaacctt ccagccggta caggcaacgc gcacgaagtg aaatacagca
     1261 ttattaatga tttcggtgtt actggcgaaa tgctgacgaa gccgattaac tgatcctttt
     1321 acaaggcgct tttactatgc attggatgca tttacctctc tatcactaca gagcgcattt
     1381 ttccttctct ctgctggcat taacgattgc cagctcgtta cctgcgtatg gcggaaaatt
     1441 taaccctaaa tttctcgaaa acgttcaggg tatcgatcaa cacatcgact tgtcggtata
     1501 cgactcacct gtcggccagc aaatccccgg taaataccgg gtttcggtgt ttgtaaacga
     1561 agaaaaaatg gcttcccgga cgctggattt tagtaccgcc agcgaagcga aacgcaaagc
     1621 cagcggtgag tcgcttatgc cttgccttag ccgggtgcaa ctggaagaaa tgggcgtgcg
     1681 cgttgacagc tttccggcgc tgaagatgtc accaccagaa gcctgcgtag cgtttgatga
     1741 aatcattccc caggccacca gccgctttga ttttaatact cagacgctgc atctttcctt
     1801 tccacaggcg gcaatgatga tgaccgctcg cggcacggtt gatccttctc gctgggacga
     1861 aggaatacct gcgctgttgc tggattacag ctttagtggc agcaatggac gcaatgaagg
     1921 tacaggttca tcttcagatt cgaccagtga cagttattac ctgaacctgc gcagcggttt
     1981 gaacgttggt ccgtggcgtt tacgtaataa cagtatctgg aaccgtaccg atggcaaaaa
     2041 ccaatgggat aacgttggta catcgttaaa ccgggcaatt attccactta aatcacagat
     2101 aactctgggt gataccgcca cgcctggcga gatttttgat agcgtacaaa tgcgtggggc
     2161 gttactggca tccgacgatg aaatgttgcc ggacagccag cgtggctttg ctccggtagt
     2221 gcgcggcatc gccaaaagca acgctgaagt gagtatcgaa cagaacggct atgtcattta
     2281 tcgcactttt gttcagccgg gtgcatttga gattaacgat ctctacgcaa cctccggcag
     2341 cggcgacctg acggtgatca ttaaagaagc cgacggaagt gaacaacgtt ttatccagcc
     2401 gttttcggcg gtggcgattt tccagcgtga agggtatctg aaatacagtc tggctgccgg
     2461 tgaatatcgt gctggtaatt acgacagcga taaaccacat ttcgggcagt ttaccgccat
     2521 gtacggcttg ccgtggggta tgaccgccta cggtggtgca ttgttgtcgg cagattacaa
     2581 cgctttagct ctgggcctgg ggaaaaactt cggtaccatt ggcgcagtat cggtggatgt
     2641 cactcaggcg aaaagtcagt tacgcaacaa tgaaaaagag gaaggccagt cttatcgctt
     2701 cctctattcg aaaagttttg agggtggcac tgacctgcgt ctgttgggct acaaatactc
     2761 aaccagcggc tactacacct tccaggaagc aactgatgtt cgcagtgacg ccgacagtga
     2821 ttaccgccgt tatcacaagc gcagccagat acagggcaat attacccagc aactgggaga
     2881 ctacggctcg gtttatttca atatgacgca gcaggattac tggaacgttg acggtaagga
     2941 aaactcctta agcgccggtt atcacggtca cattggtcgg gtgaattaca gcgtggccta
     3001 tacctggacg agaagtccgg aatgggaaga agacgatcgc ttgtggtcat tcagtgtttc
     3061 catccctctg gggggcgcgt ggagcagtta tcgcatgact accgaccaga atggcaagac
     3121 gtcgcaacag gcctctgtta gcggcacgtt actggaagat cgtaacctga gctataacgt
     3181 tcagcagggt tacaccagta atggcgtagg ctacagtggc agcgtcaaca tggggtatat
     3241 gggtggctct ggtaacattg acgtaggcta taactattct aaagataacc aacaggttaa
     3301 ctatggcgtg cgcggcggtg tcatcgttca cagtgaaggt ataacgcttt cgcagccttt
     3361 aggcgaaagt ctggcgattg tttctgcacc gggcgcgcgt ggtggacatg tggttaacag
     3421 cagcggcgta gaagttgact ggatgggcaa cgccgtggtg ccttatctca caccatatcg
     3481 tgaaaccatt gtggaattgc gtagcgacac gttggggcaa aacgttgaac tacaggaagc
     3541 gttccagaaa gtcgtgccta ctcgtggtgc agttgtgcgt tcccgctttg atacccgcgt
     3601 cggttaccgt gtgctgatga gcttaaaaca ggcgaatggc aacgcagtac cgtttggtgc
     3661 aaccgcagcg ttaattgatg agagcaaacc tgccagcagc attgtgggtg aagaaggaca
     3721 gctttatatc agcgggatgc cagaagaggg cgaattgcag gtgagctggg ggaacgagca
     3781 ggcgcaacgt tgccgtgtgc cattccgctt gccagagaac aaggacaatg cggctatcgt
     3841 gatggttaat gcggtatgcg agaaataagg atatttagat gaaaattaaa aatttgttgg
     3901 caggtatggt gctgttaggg acgagcactt ttgctggaaa tatatgggct gcggattggg
     3961 gaccatgtca gacatcaaat ggggtggccc atgagtatag ttttgatttc gtacagacta
     4021 tccaggtacc ttcagaaaat aaggctggga aaatattaac tcagcccttt gctttaggta
     4081 caaaatattc cgcttattgt gaatgcccgg atcccattcc agataatgga gttgtgactt
     4141 attttaaagg tgtaacgctt ctgcctgagc ctggaactgt agatgggtac tataaattca
     4201 ataattacat tgatattctc accaaaatat atgtctacac acaattagat atcccggtgc
     4261 cttttaccga tcgaagcaat ggcacggcac agaaagaatg tacgccctat actgctaaca
     4321 attgggggac aggtggtaaa ggaagtatca gtatttatat ttcacatcct tttgttgggc
     4381 aaatgattat tcctaaaaca cgagttgcgt ccctctttgg gacaaaaaag aaaggtgttt
     4441 acaatgatag tcagcccatg gccaatgtca gtatttccgg gagtattaca gttactcaag
     4501 gctgtgaact tgccgctggc accgtactgg atattccatt cggagaatat caggcccatg
     4561 attttaaagg gcgtgccgga caaccaccac aaaatgttca gaaggtacaa aaagaattaa
     4621 gctttgactg caataatatt tcagatggcg tcaaaattta tctcagtatt gatgcaacac
     4681 caaatactgc ctatcccagc gcaattgatt taggcaacgc cgatgttggt gcagtcattg
     4741 aggacggtaa aggtaatatt cttaagccga atgacagtaa tagcttatta gaaatgaacc
     4801 caggctcgct gtatgaagat gtcaaacgca aagcgacaac cacaattacc gcttatcccg
     4861 ttagcactac cggaaaatta cccgctgccg gggattatag cggtattgct accatacatg
     4921 tcgaactgga gtaacagtca tgaagcgaac aatacttgcg ttatgcctgc tgcctctttc
     4981 cttttcgtca ttcgcggcaa ccacggattt agggccgaaa ggaacattga aattcaccct
     5041 taaaatcagc cagggtgctt gcgaactggt gaaagacagc gttgaggttg acatgggcac
     5101 cgcggttctc aaaaagccgg taagaatcgg aacggaaatt aatcccactc ctttttccat
     5161 cggcctgaaa aactgtagcg aagtggtcag agcctacgtg actatggacg gtacacccaa
     5221 tgcagacgac tcaaatcttt ttgcgctgga tgcgggtggc gctacaggcg ttgggctgaa
     5281 aatcaaaacc gctgctggag tacaacagtt cccggaaaat acaacgccga caccggttga
     5341 gtttgcaatt aacggtgatg gcgcacatca actgaactat atcgccagct acgtgcctat
     5401 tcgcgccgat gcaactattg gtcgcgcaga tgcaacggtc gactttactg tacagtacga
     5461 ataatttcac atcttaaggg ccagttaact ggcccttttt actgcttttt gtaacgctaa
     5521 agtctctttt caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca
     5581 ctttttcgct ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc
     5641 tccgcacttt tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg
     5701 tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt
     5761 gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag
     5821 ctatgcgcac gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac
     5881 cgtactggtg accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg
     5941 tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct
     6001 taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc
     6061 aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct
     6121 tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg
     6181 gcagcgtatt cgcagcctgg cggtcgtcgt actggcgggt attatggttt gggcaccgat
     6241 tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag
     6301 ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct
     6361 gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa
     6421 gaaattcact tatcaggcac cgcaaaacgt tgatgacact tatgtcgtgt ttatcatcgg
     6481 tgaaaccacg cgttgggacc atatgggcat tttcggctat gagcgtaata ccacgccgaa
     6541 actggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa
     6601 actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac
     6661 attaaaagaa cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta
     6721 cgctatgcag agcgaaatgt ggttctacag caacacgatg gcggacaaca ttgcttatcg
     6781 tgagcagatt ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt
     6841 agacgaaatg cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca
     6901 taccaaaggt tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa
     6961 gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga
     7021 caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa
     7081 gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct
     7141 gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg
     7201 gatgtcagat aaatatctgg aaaatccggc caatgctcag gcatttgcgc agctgaaaaa
     7261 agaagccgac atgaaagtgc cacgccgtca tgtagagctg tacgacacca tcatgggttg
     7321 tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc
     7381 gcagacaaaa gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg
     7441 cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc
     7501 ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat
     7561 cctctatcac cgaccaaatt cgaaaagcct gctcagcgag caggcttttt tgcatttgaa
     7621 gcccatgagg atgagaacct ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac
     7681 gttgcgccag caacggcccg cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg
     7741 accaaattcg aaaagcctgc tcaacgagca ggcttttttg catttgtagc tcatgaggat
     7801 gagaacctcc gggggcgggg aggttcgact cgacgtagat actgataaga cgcgccagcg
     7861 tcgcatcagg caacggcacc gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc
     7921 tacacaccac acatttccct gccaaatttg cgccccatat ctcactatcc attaacggat
     7981 ttgtgacaga aaccattgtc tttttttata tttgcttaaa tctttttttg cgttacttat
     8041 ggctgaccct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
     8101 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
     8161 aaatattaag ctgattgttc tggtcatgtt ggagtagcac aaatttctct gctgcaaata
     8221 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
     8281 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
     8341 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
     8401 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
     8461 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
     8521 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
     8581 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
     8641 ccacctatga cgcctcttcc gtaccgcttt ataaccgcct ggttgaattt aaaatcggca
     8701 ccactgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
     8761 ataccttcca tctgcgtaaa ggtgtgaagt ggcatgacaa taaagaattc aaaccgacgc
     8821 gcgaactgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
     8881 cgtaccataa agtttctggc ggtagctacg aatacttcga aggcatgggc ttgccggagc
     8941 tgatcagcga agtgaaaaag gtggacgaca acacagttca gttcgtgctg actcgcccgg
     9001 aagcgccgtt cctcgctgat ctggcaatgg acttcgcctc tattctgtca aaagaatatg
     9061 ctgatgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
     9121 gtccattcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
     9181 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
     9241 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
     9301 cagatatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
     9361 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
     9421 aggcactgac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
     9481 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
     9541 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
     9601 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
     9661 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
     9721 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
     9781 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
     9841 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
     9901 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
     9961 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    10021 cggtacgcaa agaagttaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    10081 acgtctctat cgaataatta aaagccatac ccggtggcgc tgtgcctctg cgtgcagcgc
    10141 catccggcag caatacttca ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat
    10201 acagacacgc agttccaggc tgcgggtcac tatagagaat ccgggttatg ttgcagttta
    10261 ttctccgacg tttgggactc gtcatcccca cgtttatcgg tattaccctt ctcacatttg
    10321 cctttgtcca catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct
    10381 cccctgagcg tcacgcgcag ctgctggctg aactcggctt agataaaccg atgtggcagc
    10441 agtatctcca ttacatttgg ggcgtaatgc acggcgatct aggcatttca atgaaaagcc
    10501 gaattccagt atgggaagag ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct
    10561 gcgcgatgat ttttgctacg gcagtcggta ttccggttgg tgtgctggct gcggttaaac
    10621 gcggttccat tttcgatcac acagcggttg gcctggcgct gaccggctac tcgatgccga
    10681 tcttctggtg gggcatgatg ctgatcatgc tggtttcggt gcactggaac ctgacgcccg
    10741 tctccggtcg cgtgagcgat atggtgttcc tcgatgactc caatccgtta accggtttta
    10801 tgctaatcga caccgccatc tggggtgaag acggcaactt tatcgatgcc gtcgcccata
    10861 tgatcttgcc tgccattgtg ctgggtacta ttccgctggc ggtcattgtg cgtatgacac
    10921 gctcctcgat gctggaagtg ctgggcgagg attacatccg caccgcgcgc gccaaagggc
    10981 taacccgcat gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg ccggtggtga
    11041 ccgttatcgg cctgcaggtg ggaacattgc tggcgggggc gattctgacc gaaaccatct
    11101 tctcgtggcc cggtctggga cgctggttga ttgacgcact gcaacgccgc gactatccgg
    11161 tagtgcaggg cggcgtattg ctggtggcga cgatgattat cctcgtcaac ttgctggtcg
    11221 atctgctgta cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt
    11281 cacaggttac tgaaaataaa gtgattagcg caccggtgcc gatgaccccg ttacaggagt
    11341 tctggcacta ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac gtcgtcatcg
    11401 tgctgttcat cgcgatcttt gccaactgga ttgcacccta taacccggcg gaacagttcc
    11461 gcgatgcact gctcgccccg ccagcctggc aggaaggcgg cagcatggcg cacttgctgg
    11521 gcaccgatga cgtaggccgt gatgtgctgt cgcgcctgat gtacggtgcg cgcctgtcgc
    11581 tgctggttgg ctgtctggta gttgtgttat cgctgattat gggcgttatt ctcggcctga
    11641 tcgccggtta ctttggcggc ctggtcgata acatcattat gcgcgtggtc gatatcatgc
    11701 tggcgctgcc aagtctgctg ctggcgctgg tgctggtggc aattttcggc ccgtcgattg
    11761 gtaacgccgc gctggcactg accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg
    11821 ccgccgtgct ggtggaagtt aaccgcgatt acgtcaccgc gtctcgcgtg gcgggtgccg
    11881 gggcgatgcg tcagatgttt attaacatct tcccgaactg ccttgcgccg ctgattgttc
    11941 aggcgtcgct cggtttctct aacgccattc tcgatatggc tgctcttggt ttcctcggca
    12001 tgggggcaca gccgccaacg cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg
    12061 cgcaaagcgc ctggtgggtc gtgaccttcc cgggtctggc gatcctgctg acggtgctgg
    12121 catttaacct gatgggtgac ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag
    12181 aggttcgaga tggcgttatt aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc
    12241 gcgccgttcc gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga agtggtcggg
    12301 attgtgggtg agtccggctc cggtaagtcg gtcagttcac tggcgattat ggggctgatt
    12361 gattatccgg gccgcgtaat ggcagaaaaa ctggagttta acggccagga tttgcagcgt
    12421 atctcagaaa aagagcgccg caacctggtg ggtgccgaag tggcgatgat cttccaggac
    12481 ccgatgacca gccttaaccc gtgctacacc gtgggtttcc agattatgga agcgattaag
    12541 gtgcatcagg gcggcaacaa aagtacccgc cgtcagcgag cgattgacct gctgaatcag
    12601 gtcggtattc ccgatccggc atcgcgtctg gatgtttacc cgcatcagct ttccggcggc
    12661 atgagccagc gcgtgatgat cgccatggcg attgcctgtc ggccaaaact gctgattgcc
    12721 gatgaaccga ccaccgcgct ggacgtgacc attcaggcgc aaatcatcga actactgctg
    12781 gagctacagc agaaagagaa catggcgctg gtgttaatta cccatgacct ggcgctggtg
    12841 gcggaagcgg cacataaaat catcgtgatg tatgcaggcc aggtggtgga aaccggtgat
    12901 gcgcacgcca tcttccatgc gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg
    12961 ccagaatttg ctcaggacaa agaacgtctg gcgtcgttgc caggtgtcgt tcccggtaag
    13021 tacgaccgcc cgaacggctg cctgcttaac ccgcgctgcc cctatgccac tgacagatgt
    13081 cgcgctgaag aaccggcgct gaatatgctc gctgatgggc gtcagtccaa atgccattac
    13141 ccacttgatg atgccgggag gccgacacta tgagtacgca agaggccacc tcgcaacaac
    13201 cgctgttgca ggctatcgac ctgaaaaaac attatccggt gaagaaaggc atgttcgcgc
    13261 cggaacgtct ggttaaagcg ctggatggca tttcgtttaa ccttgaacgt ggcaaaacgc
    13321 tggcagtagt gggcgaatct ggctgcggta aatcgaccct cggtcggttg ctgacgatga
    13381 ttgaaatgcc caccggtggc gagctgtatt accaggggca ggatctgctt aagcacgatc
    13441 cgcaggcgca gaagctgcgt cggcagaaaa tccagatcgt cttccagaac ccttacggtt
    13501 cgctgaatcc gcgtaaaaaa gttgggcaaa ttctcgaaga gccgctgctt attaacacca
    13561 gcttaagcaa agaacagcgt cgggaaaaag ccctgtcgat gatggcgaaa gtcggcctga
    13621 aaaccgagca ctatgaccgc tatccgcata tgttctccgg cggacaacgt cagcgtatcg
    13681 ctattgcccg tggtctgatg ctcgacccgg atgtggtgat tgccgatgag ccggtttctg
    13741 cgctggacgt ctcagtgcgt gcgcaagtgc tgaatctgat gatggattta cagcaggaac
    13801 tggggctttc ttatgtcttt atctcccacg acctgtcggt ggtggagcac attgccgatg
    13861 aagtgatggt gatgtacctg ggccgctgcg tggagaaggg aacgaaagac caaatcttca
    13921 ataacccgcg ccatccgtac actcaggcgc tactctccgc gacgccgcgc ctgaacccgg
    13981 acgatcgccg cgaacgcatc aaactcaccg gtgaactgcc aagcccgctg aatccaccgc
    14041 cgggttgcgc cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc
    14101 cgcagctaaa agactacggc ggccaactgg tagcttgttt tgctgttgat caggatgaaa
    14161 atccgcagcg ttaactcaca aaaccggggc catgcgctcc ggtttactca atcagcttca
    14221 acagtggcga caagcatagc aaaatgccgt aaagcagaat tagccaggct ttaaatccgc
    14281 gaagtttttc cagttgtgcg actttataga tcaggaaaaa cggaatgagg cacgacacaa
    14341 tgccatataa cgggcttccc aactgaaaga acaccagcac cgatacacga aacgaaaccc
    14401 aaatcgtcaa tgtaatgacg ataaaagcgc agatcgccag agtcagcatg cgtgagttaa
    14461 ttttcttggt atcaataatt cggcttaaca gattgagaat aatgccttta atagcctcgt
    14521 ggaaaccgag ataaatgcca aagaatgcgg tcagtacggc aaagatattc agcaccgtag
    14581 aggtgatatg aatgatatgt ccagggatca cctgcgcggc cagcgccagt gccgagatat
    14641 tttgttcaaa ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa
    14701 aaaacaggat caccgcgatg agcgtgatat aactaattcg gtgggtacgc agcgcgagcc
    14761 gggttgccag tactttatcc gcttcccgtt tacgataggc aatattcatt gggttaagta
    14821 cctgaataaa tactgcagaa aagaagcaaa atggaatggt aagcaagaca tcgcggaaaa
    14881 agacggaggc ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa
    14941 aaccgaacac gacaataatc cctactttga ccaccaccat tggcccggaa atcttaaaca
    15001 gtaatcgttc accaccagac gcaatcgcca ccagtacggc gaaaatagcg actttataaa
    15061 gtagagattg tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt
    15121 cgaaaaccac ggagagagag tagataaaaa tcccgtggat aatcatcaaa aagtagataa
    15181 ccccgaggaa aattccccag ttcttcccca ggtaatgact gataatatcg gtgtagtcat
    15241 tacaggaatc gctttcagaa agggttttta aataaatgtc ctgcactacc caggtggcag
    15301 gataagcaat gatcgctgcg gtaataaata cccaaattcc cttcaagcca atttgtactg
    15361 gcatcagcac ggttccggca ccaatcgcca tgcctatgca taataaaacc cagccgaagt
    15421 cgtagcgtgt aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt
    15481 cctgtccaga aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca
    15541 ggtggagaag tcgaatacgc agtatgtaaa aaatgaggca acagattcac tggggaggtc
    15601 gcgaggaggc gcaattctac aaaattgcgc atttcactac cagtctggag tcagcccgga
    15661 tatgaactgc aactattgca aataaagaaa ttaagctgga aggcatcgcg caatgaggaa
    15721 gattgccggg tgctgacagt aaaaagcaat cgtaaaatca gcggcaagcc gaaagggttc
    15781 cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct ggagagagaa
    15841 aacccccgca cgttgcaggt atgtacctga caacataacg ggggctaatc ttgaacctag
    15901 accactcaag aatagccgcg aaacgttgtc attacaacac aggcggctat atgacgctcg
    15961 cagagctggg catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg
    16021 ccagtatgat cgtgaactgg ctgaacaagc ggaagtagcg tgtcatgtgg gcgtcaggct
    16081 gccgtaatgg caatttgcgc ccggaccagg ccgcaggagt gaaactctgc ggcctttttc
    16141 gtaagaaaaa tgcaacggtt gcaagcggat aatattctgc ggaatgcgca gcacagagtg
    16201 aagatgagac ggattccgga acagaaaaac agtcgtagaa tcagcgtcaa gccggaagga
    16261 ttccggtgag gcgcaatgtt gcgggggctt aatccctggt ggcattggtt gctggagaga
    16321 gaaaaccccc gcacgttgca ggtatgcacc tgacaacacc acgggggcca ttcttgcatc
    16381 caaacaatac aagaatagcc gcgaaacgtt gtcattacaa tacaggcggc tatatgacgc
    16441 tcgcagaact gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggtattc
    16501 ttgccagtat gatcgtgaac tggctgaaca agcggaagta acgtgccatg cgggcgtcag
    16561 gctgccgtaa tggcaatttg cgcccggatc aggccgcagg ggtgaaactc tgcggccttt
    16621 ttcgtaagaa aaatgcaacg gttgcaagcg gataatattc tgcggaatgc gcagcacaga
    16681 gtgaagacgt gacggattcc ggaacagaaa aacagtcgta gaatcagcgg caagccggaa
    16741 gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggag
    16801 agagaaaacc cccgcacgtt gcaggtatgc acctgacaac atcacggggg ccattcttga
    16861 acctgaacaa ctcaagaata gccgcgaaac gttgtcatta caacacaggc ggctatatga
    16921 cgctcgcaga actgggcatg gccttctggc atgatttagc ggctccggtc attgctggca
    16981 ttcttgccag tatgatcgtg aactggctga acaagcggaa gtaacgtgtc atgcgggcgt
    17041 caggctgccg taatggcaat ttgcgcccgg accaggccgc aggggtgaaa ctctgcggcc
    17101 tttttcgtaa gaaaaatgca acggttgcag gcggataata ttctgcggaa tgcgcagcac
    17161 agagtgagta agtgatggat ttcggaacag aaaaacagtc gtagaatcag cggcaagccg
    17221 gaaaggttcc ggtgaggcgc aatgttgcgg gggctttatc cctggtggca ttggttgctg
    17281 gagagagaaa acccccgcac gttgcaggta tgcacctgac aacataacgg gggctaatct
    17341 tgaacccaaa caactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata
    17401 tgacgctcgc agagctgggc atggccttct ggcatgattt agcggctccg gtcattgctg
    17461 gcattcttgc cagtatgatc gtgaactggc tgaataagcg gaagtaacgt gtcatgcggg
    17521 cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcagaggtt aaactctgcg
    17581 gcctttttcg tgcttactgc gggtaaggca cccagtcgcc gccgttcagg cgaacgtacg
    17641 gtttatcctg gtattgaata actactgcat ttgagttctc ggagaccggt gctgtttgtg
    17701 gcaacccact ggtgagtttt ttccagtcaa cattgtcttc ggtgaaaatc ttgccatcaa
    17761 gaacgcgaac caccagatcg gagatagcca ggaagctgct cggttggtca atgacaatcg
    17821 gtgccccctg atgtggtgcc ttcatgccga agaatttcac cccaacgggg acgtctgtga
    17881 tagacgggct agggatatca cgtaggccag atacctgcat tctgtcgccc ttaagcgcgc
    17941 cgccgtgttc cggcaccacg accaccatca ctttacgacc cgatttctcc agttcagtaa
    18001 agaaggcgtc cagttcatca aagaatttct gcgcccgcgc tttgtaatcc gctgttttgc
    18061 tgacccccgg ataatggttg ccgtcatgca gtggaagcgt gttgtagaac gtggcactac
    18121 ggctgttttt atctttttcg gtaacgtcca gccagcggtt aagcacggcg gtatcgtcat
    18181 aaaccggcga accatcaaag cccagcaaaa taaccggcag atttgtttga tccatcaatt
    18241 cagtctgcat gccgccattt tcgcgaactt ctttcaaaaa accgccgaac tggccgttat
    18301 gccccatcat caggtgctgg gtaaagccca gtttcgaaag gttatcaaac agatagcagt
    18361 cgttatttgc cggttgatac agattagtgt gcgaagtctg cccgcagctg gcgcgcagta
    18421 aacggatcgc cgccgggcca ctgtaggagg tggcggagtt aaagttcttg aactcaatat
    18481 cgaaatgcga ccacagtgga tgcgacatca acccggcggc ttctatatcc gaccaggaaa
    18541 gcgaacagat gttaatcacc agtagttcaa atggctgagc atcagcgggc agcgaagacg
    18601 ggaaggtcga tttacgtttc gcctccgcgt tatagaaatt attcagccag gcgttaaggt
    18661 tcgccgttgt tggcggtgca gtttgtgcgg gcatatcacc cactaccggt gcgccacccg
    18721 tcgccgcaac ggttgccgct gcgttaccac ccgtcgttgt tacagtggtc gtcggttgtc
    18781 cggctggcca caaggagaaa cttggtcccg ccagggtaag tacgttcagc catagcagta
    18841 tggcaaccac aaaaacggta atgcgaatcc attgtgacag gaataaccag gccactaata
    18901 aaacaaaaat ggccccaatc atctgccagt taataaagcg tgtgacaagg tcgattaaat
    18961 aatcggtact gaaccccgcc acctgcgaac cctggctcat tatgctttcc gggccaggca
    19021 accaggtgtc atgccagaac aaagcaaagc cgatcggcag ggcaatccag tggcgcaagc
    19081 gatgcaggct gtagcgcgga atgggcatca gcagaaacgc ggcaaacacc aaattgagga
    19141 gcggatggaa gttaagatat cccgcccaca acaggccgaa cttaaccaga aaatagaagt
    19201 tccagccgga aaggccgcgc cagtattgcc agagggaaga aggcatggcg gtattttgcg
    19261 taaattgagt cattttttgg ttgccctggc tttttccgtg cggcgtaacg tcccggccgg
    19321 tttaacataa cgaggtttag caaagaacaa acgtaaggtg tcgcgaatgc gtcgcaaaga
    19381 gtgccgcgcc agatagccca gcgggaaaaa tatcagtgcg caaacgacaa taatttcaat
    19441 gatatcgctg atggtcatca tgatgagcgc tccacagcat catctaacag tcgcatgggt
    19501 tctggtattc gtcgccagtg gcgaccatcg tgctcggcat tgatgaccgg tttagaactt
    19561 tgcgttaaag gcagcggcat gccccattgt tctggggcaa gcaagcgcat ctgcaccagc
    19621 tcggcactga tttgatcatc ttcaaaccag accatacggt ttgagaaaat gtcgccagta
    19681 ggcaatggga aaatatgatt caacgcggta tccagatcgt taatccgaca gaatgagaga
    19741 aacagcacca gccgattacc gccaatggtc atgatatcgc cggtacggtt agggcgacac
    19801 agcgtcaggg cttgttcaac gcggataccc ggtaccggac gtagggcaac cagaacgcct
    19861 ttaccgtggg caggtaatag agggttattc atcatgttgt tgacggcatt acagaacaca
    19921 tcccacttct ggaaaccacg cagtttgagc ggctgggtca ttgacagcaa ggtagtgata
    19981 tcttccggca catagcgact aaacttctgc ccttgcacgc tttcgatcat cgtcagacaa
    20041 cgggagagtg gcgcattcca cggaataacc atatttgcac cgcaggccaa taacaaacgt
    20101 tcatcggtgg cgcgcaggct agcggtattt tcccgcacga ggattttcat cgcactaccg
    20161 cgctggcgac gcagggtatg aatgctgcgg gccagtggct cgatttgcgc attttgctgt
    20221 aaagaaaaga ccaccgtcgc cgcctgagcg gtacgggctt cattgaacag gacttcgtta
    20281 ttgttgaaca gttgccagtg ttccgatagc ggcggcgcac cttccagtac agcaacatta
    20341 ctcagaatgc gtttttcgtc gctgcgtggt tggatctccg cctcttcgct ttgaactaat
    20401 gtccagatac cattttgttg ctgaacgcta agctgctgac gggcgctgac ccctttttcg
    20461 ttgcaccaga aggcaatatc cagcaaatgt tggtcaccct gaaaacgcaa actggcaaga
    20521 ccaaaaagtg aacggtattc ctcaagcaac aatgaaaatt gtttatcgtt attatttccg
    20581 ggattaatta ccaaaagcga acaatggttt aacctgctcc atttattcat tttatccaac
    20641 catgagcgaa gccgctcggc aggaatgttt tgccatgcgt tatttgcgca aacaagaata
    20701 aaaaggtaat tatggggatc aatagaacac tgcaaatcac gggtcaaata gtatagacct
    20761 ttttcatgat ttagcattga aaataatttt attttttccg gcccttgaga atcatctaat
    20821 tgaaagattt tcgccggatc gctgtccatg ctaatgaccg cgacgtgtgc ggtttcagcc
    20881 tgggatgcaa ttgtttgatt cgccagactg atagcatctt catggcgatc gacgttaaac
    20941 caccagacgc cgcctgctgg catatgtcgc agctcatccc ataatgatga gataccgata
    21001 gagaatacag ggtccacaat gtccctcatg tcaccatttt gtctgattat cagtttacta
    21061 gcgaaagcta agaaataaac ctaccattga aattaaaact tatcagattt agcatgtgaa
    21121 tcaatttaga aggtcacaag acgtgtgatt ttagtctttt cgtttttaac tttttctgtt
    21181 gcgtaacgga aagtcaaaaa gtgagcaaat tcccgtctcg ccgtaaacaa taaaccggcg
    21241 aaatgctcac ggttagaatt gtctcatgaa cggtacggtt atttcatagg gatcaagcaa
    21301 aatgaataac aatgaaccag atactctgcc tgatcccgcg ataggctata tcttccagaa
    21361 tgatattgtg gcgttaaagc aggcattttc actgcctgat attgattatg ccgatatttc
    21421 ccaacgcgaa cagttggccg cggcattaaa acgctggccg ttgctggcag agtttgcgca
    21481 acaaaagtag gggattggtg aatggccgta ct
//
LOCUS       DAZFWP020000080        21447 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFWP020000080 DAZFWP020000000
VERSION     DAZFWP020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.080"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..315
                     /locus_tag="ACHK0Z_RS14370"
                     /old_locus_tag="pgaptmp_002874"
     CDS             <1..315
                     /locus_tag="ACHK0Z_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4502865.1"
                     /translation="EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTN
                     TELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSL
                     AG"
     gene            complement(358..1509)
                     /gene="ydiO"
                     /locus_tag="ACHK0Z_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             complement(358..1509)
                     /gene="ydiO"
                     /locus_tag="ACHK0Z_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU4502866.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1523..3118)
                     /gene="ydiF"
                     /locus_tag="ACHK0Z_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /pseudo
     CDS             complement(1523..3118)
                     /gene="ydiF"
                     /locus_tag="ACHK0Z_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3262..4020)
                     /gene="aroD"
                     /locus_tag="ACHK0Z_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             complement(3262..4020)
                     /gene="aroD"
                     /locus_tag="ACHK0Z_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU4502867.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4051..4917)
                     /gene="ydiB"
                     /locus_tag="ACHK0Z_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             complement(4051..4917)
                     /gene="ydiB"
                     /locus_tag="ACHK0Z_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU4502868.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4929..6194)
                     /gene="ydiN"
                     /locus_tag="ACHK0Z_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             complement(4929..6194)
                     /gene="ydiN"
                     /locus_tag="ACHK0Z_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502869.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6421..7635)
                     /gene="ydiM"
                     /locus_tag="ACHK0Z_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             complement(6421..7635)
                     /gene="ydiM"
                     /locus_tag="ACHK0Z_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4502870.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFFSFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7735..8091)
                     /gene="ydiL"
                     /locus_tag="ACHK0Z_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             complement(7735..8091)
                     /gene="ydiL"
                     /locus_tag="ACHK0Z_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU4502871.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8227..8334)
                     /gene="rprA"
                     /locus_tag="ACHK0Z_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     ncRNA           complement(8227..8334)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK0Z_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8520..9632)
                     /gene="ydiK"
                     /locus_tag="ACHK0Z_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             complement(8520..9632)
                     /gene="ydiK"
                     /locus_tag="ACHK0Z_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU4502872.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10021..13077
                     /gene="ydiJ"
                     /locus_tag="ACHK0Z_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             10021..13077
                     /gene="ydiJ"
                     /locus_tag="ACHK0Z_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU4502873.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIVSQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQISI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13074..13484
                     /gene="menI"
                     /locus_tag="ACHK0Z_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             13074..13484
                     /gene="menI"
                     /locus_tag="ACHK0Z_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU4502874.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13584..13772
                     /gene="ydiH"
                     /locus_tag="ACHK0Z_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             13584..13772
                     /gene="ydiH"
                     /locus_tag="ACHK0Z_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU4502875.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14320..14688
                     /gene="sufA"
                     /locus_tag="ACHK0Z_RS14435"
                     /old_locus_tag="pgaptmp_002887"
     CDS             14320..14688
                     /gene="sufA"
                     /locus_tag="ACHK0Z_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU4502876.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14697..16184
                     /gene="sufB"
                     /locus_tag="ACHK0Z_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             14697..16184
                     /gene="sufB"
                     /locus_tag="ACHK0Z_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU4502877.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16194..16940
                     /gene="sufC"
                     /locus_tag="ACHK0Z_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             16194..16940
                     /gene="sufC"
                     /locus_tag="ACHK0Z_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU4502878.1"
                     /translation="MLSIKDLHVSVEGKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16915..18186
                     /gene="sufD"
                     /locus_tag="ACHK0Z_RS14450"
                     /old_locus_tag="pgaptmp_002890"
     CDS             16915..18186
                     /gene="sufD"
                     /locus_tag="ACHK0Z_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU4502879.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPFLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18183..19403
                     /gene="sufS"
                     /locus_tag="ACHK0Z_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             18183..19403
                     /gene="sufS"
                     /locus_tag="ACHK0Z_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU4502880.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19416..19832
                     /gene="sufE"
                     /locus_tag="ACHK0Z_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             19416..19832
                     /gene="sufE"
                     /locus_tag="ACHK0Z_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU4502881.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19981..20985
                     /gene="ldtE"
                     /locus_tag="ACHK0Z_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             19981..20985
                     /gene="ldtE"
                     /locus_tag="ACHK0Z_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU4502882.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21049..21285)
                     /gene="lpp"
                     /locus_tag="ACHK0Z_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             complement(21049..21285)
                     /gene="lpp"
                     /locus_tag="ACHK0Z_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU4502883.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5581 a         5220 c         5426 g         5220 t
ORIGIN      
        1 gaacagttat cgaaaaaatt tcgtgccagc gtcagttata tttgccatga atttaccaaa
       61 gagtatcgta tttcccctat taactatgtt attcaacggc gtatgacgga agcgaaatgg
      121 tcactcacta atactgaatt atcacaggca gagatctcct ggcgtgtggg ttatgaaaat
      181 gtcgatcact ttgccaaact gtttttgcgc catgtcggtt gttcgcccag cgattaccgc
      241 aggcaattta aaaactgttt tgcggaacaa gaaatcctat ctgaatttcc tcaaccggta
      301 agtcttgccg ggtaaatctg gcggcgcagt ttgttaagaa gctgcgccgc ctgcagatta
      361 tttgttctga taatctttca ggatctgccg acccgcaacg taaatcataa tttcgtctgt
      421 accgccgccg atacgttcac aacggacatc acgccagaag cgggagacgc gcgcctcatc
      481 ggtatagccc agaccgccca tgatttgaat cgcatcatca atgacttcca ttgcggtacg
      541 tgcacagtac agttttgcca gcgccgcgct ggtgcgcagt gactgatgct gatcggcttg
      601 ccatgccact ttcagcacca tgttgcgcat gttgtcgatc ttaatcgcca tcagcgccag
      661 tttttcctgg atcatctggt tatgaccaat gggcttacca aaagcgatac gttggttggc
      721 atagcgggcg gcatcttcaa aggcgcattc ggcaaagccg gtgctgcgcg cggcgttgat
      781 caggcgctcc atctcaaagt tgtacatcac attgaggaaa cccattcctt cttcgcccac
      841 catgtcgctc tcttcaacct ccacgttgtc gagatagact tcgcaggtgc tgagcatatg
      901 ccagccgatt ttatgcagcg ggttaatctt aatgccgggc ttactggagt cgacccacca
      961 cagggtgaag gcttttttgg gatctttcgg ttgcggatcg cgcgccagca ccagcatata
     1021 cgggtactct ttcgcaccgg taataaaggt tttttgtccg ttgatgtaga ccttgccgtt
     1081 tttacgcgtg taagtggtag tggcactgtt gttatctgag ccagcacctg gttccgtcaa
     1141 cgccagggca taggcgggat cacccgtttc cagggtgctc tctgccgttt tacgtagctg
     1201 ctctgcagaa ccgaaacggc gcatactgtg aatacattga ccgttggtaa ttaaaaatgc
     1261 cggagcaccg cattttgaca cttccatcag cgccagcatt tgggtgacgt aatccgcagg
     1321 gataccacca aattcttccg gcacgccaag catggaaata ccgttatccg ccagcgcccg
     1381 cataaactca cgcggatatg tcccgttttg atcgcaggtg cggaaatact cttccggaaa
     1441 gttagtcgta atcagttcgc gaatactggc cagcagcagt tcttgttctt cagttaaaga
     1501 aaaatccatc attatactcc tgttaatgag ccgcttcagg caggacaaaa cccatcgccg
     1561 catcgataaa taatctttcg tccatcagtt tgagttctgg cgaaatcact ggggtgaagt
     1621 ccattttgtc gagaatatct ttttgtaaat cgacgccagg ggcgatttcg attaaatgca
     1681 ggccgtcttc tttcagcgtg aatacagcac gttcggtgat gtaacgaaca tccagtcccc
     1741 gttcgagcgc gatttttccg ctaaaagtaa tttccggtag ttcccgaata aatttattta
     1801 cccgtccttc ctggacgata tgcaatttgc catcggcaat ttctgttttt aaactgcccg
     1861 cagttaacgt gccgcagaaa ataattttct tcgaggtggc actgatatca ataaatccac
     1921 cggtacccat gattttgcca ttgaatttat gcacgccgac gttaccgtgc tggtcgactt
     1981 cagcaaaact caaataacaa acatccagac caccaccgtg ataaaaatca aactgggacg
     2041 tcatatccag aatggcgcgg gtattcacgt tcgcgccaaa ggcgataccc tgtgaagtaa
     2101 ttccgccaat cggacctgtt tctaccgtca gaataaagtc atcagcacaa ccttcttccc
     2161 gggcgaccag gccaataccg tcagcaatac cgacgccgac attccccacc gcgcctttac
     2221 gcatttcgaa taatgcacgc cgcgcaacta atttacgttg gtttaggggc aggctaagtt
     2281 tggtgctgtc atcaagggtg aagtcaccag aaataaaacg gttaaccggc gcaccgccat
     2341 acagttggct ttgatccgga tcgaccacca caatatccac cagataaccc ggaatacgga
     2401 cagatttagg atgcagcgtg gctttcttaa ccattttctg cacctgcatc atcacaatac
     2461 cgccgttatt gtgcaccgcc tgggcaataa ccaatgcgtc gagatacatc acctcatctt
     2521 caaaagtggc gtagccttca ctgtcgcagg tggtagcgcg aatgaaggcg atatctggcg
     2581 caatcgcttt gtaatagaga tattctttgt tatcaaactc gaccagttta atcaggtctt
     2641 ctttcgtgac ttcattcagt ttgccgcctt gctggcgtgg atcgacaaat gtcccgatgc
     2701 caatatcact aataatacca ggctggtggg ctgcggcggc gcgtaaggtt tgtgtaagta
     2761 caccttgtgg atagttataa gcaataattt tattttgttc tgcgagttca gaaatacgcg
     2821 gcgattgtcc ccagtgacca cataatgccc atttcaccag accttcttgc gccagaggac
     2881 taataccacg gtcggcgcga tcgccaagcc ctgtcggact aataatcgat aaattacgtg
     2941 gcgtttgagt ctgtttatat ttatcagcaa gagctgtaat taacgtggtg gcttacagaa
     3001 taccgccgcc agcgcctaac acacaaagtg ttgcttcgtc gggaatataa ttcaccgctt
     3061 cctgtgccga caggaccggc actcgtccat taatacgagg tggttttaca ggtttcattg
     3121 ttaattcctg attcagatgt gattttccct acgccagatg gagaattagc gcacagagac
     3181 tcacgaaata tttttattaa atacggttaa atatattttt taagttcggc ggggagggtg
     3241 ttcccgccga aatattattg cttatgcctg gtgtaaaata gttaataccg tgcgtaaatc
     3301 atttaccgag atttgccctg gtgcagaggc tttttttacc gcaccaaaag ttgccgccga
     3361 gccaaatact tcaccagcca gacgagaaat tacgccagtt tttgccatcg acatcgtgat
     3421 gattggacga tcggcatact gctcctgcat ctccagggtc gcggtaagca acgtcagcac
     3481 atcgctggta ctttgcggca tcagcgcaat cttaggaata tcggcgtcga aggactgcat
     3541 tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt ttatggaagt catggttgga
     3601 catgacaact ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa cctgatcatc
     3661 gccggtaaat aactccagat cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt
     3721 gagagcgata taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa
     3781 ggtaaacagc agcggttttt ctggcatggt ttcacggaga atttttgccg ccgccatgac
     3841 agactccaca ttggagaggt cggcaaagtg gtccacacgc cattccagaa tatcaaagtc
     3901 cgcttcacga taggcgagag cttcggattt cacgcgggcg atatctttcg ccatcagcga
     3961 gacgatgatt ttaggtgcgc ccgtaccaat gacgagatct tttacagtta cggttttcat
     4021 tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga cctgtttaac
     4081 atattccaga gggaaatctt tgccagtcca taacgtgaac tgttcagccc cttgccacaa
     4141 caacatgccg tatccatcaa tcgttttgca accagcttgt tgcgcctgct gcaataactt
     4201 cgtcatatgc gggttataca cgcattcagt gaccagcagt cccggatgta acagactgat
     4261 atcattaact aatgattcat tctcaagggg tttcataccc acttttgtgc cattggttaa
     4321 aatgtcggcg gaagccaggg cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt
     4381 gacgacacaa tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa
     4441 ctcatcccga cggttaaaga gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc
     4501 aatcgccgtt gaggcacctc cggcccctaa cagcaccatt gttttgcctt tgatatcaaa
     4561 accgctctct ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc cgcgcaggta
     4621 gccatcatca ttaacgatgg tgttgatggc acccaccagt ttggctgctg gcgttaattc
     4681 atcaacatat tcacacgcca gttgtttgtt cggcattgat ataccagttc cgcgcatttt
     4741 gagggctttt aatccttcaa ttgctccggg aaaactatcg ttatccactt caaaggccat
     4801 ataggtaaat ggcaatcccg ctttttctaa ggctttattc tgcatttcag gcgataaact
     4861 gtggcggata ggataggcca tcagcccaat caattcgtat tttgcggtaa catccataat
     4921 taactccttt aacctctatg cttaattgtg tttaaccggg tgctaaattt acgctcgcca
     4981 aaccgcacat cattctcagg aataataaaa acgcggtaat agcggataaa aacaataatt
     5041 gcggtaatca gggccagcag cgcgaaagta aaatcgagaa caatgatata ttgaaggccg
     5101 atgttcgaca gataaccggt aatcagcgga ataacaaagt tagccagtcc acccatcatc
     5161 atataaatac tggtgacttt ggctttgctt tttgggaaaa actctgacat gaccgaaaca
     5221 ccgagctgta aaatgccgcc agctgctgag aaaccgataa caaaggcgcc ggcattacac
     5281 accagtggag aagggtacag gtagataatg gctgctgtta ttgttgccag tgcagagtta
     5341 aatacattag cccagatggg ccggaccatt tttttcagta gtgcggcaaa aataaagaca
     5401 cagaccaacg agcccatact gtaataagag atggttttta atgcctcagc ttctgacata
     5461 ccagcaaaag ccatcgcata tttgggcatc cacaccacaa tcacataaaa ggtcgagaat
     5521 gcggctacac cgaacagtac cgatgaaaca ccttccagcc agactaacgg tttgctgttc
     5581 atttgcggta attcattagc tacgctggcg tccaccaact ggctggggaa tttgcttttc
     5641 aacagcatca gcgtgatcag tacaaacaga atacccggaa taatcagccc atagccgtac
     5701 cagatattat tgagcaacat atagctcacc agcattgggt agaacatttg cccaaatgac
     5761 accatcgctt taaccagtat gaccgccgaa ccagaggctt tcggaaagca ttccatgagc
     5821 gcggggtagc cacccgtatc cagcgctgag ttagcgatac ctacgcacac tgccagaccg
     5881 taggcgagag ttaaattcgg gcaagcggga ataccaaaga agaatagcag atacattatt
     5941 actgccatta atatcaccgc ccgacgacca aacttatcgg agatcacacc gaagaataaa
     6001 atactgatca atcgccccaa accgataccg gaaattaagt aggcaatgcc cgcgttgtca
     6061 gtggaaaact tttctgccag agatgacata ttttgggcaa gcgtaataac actaatgccg
     6121 tgcaggaaat agctgaagta aatacaaaga acagccagga taaatggcgt gctgaaagcc
     6181 ttattttgag acattttcac agctccttgc tgaaagataa tttcgttacc gtacgtattg
     6241 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     6301 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     6361 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     6421 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     6481 taagccgata aacagtgcca gtaaaaaacc gatggcagcg atggcggtat cgaaccacat
     6541 aatatcggca atacttcttt gggacagatg agccgtaatc aacggaatag taaaggtcgc
     6601 aatactgccc gcactgtaat agatccctgt agctttacct ttagcgtaag ggaaacgttc
     6661 agccattaac gtcaggccaa tttgcacaac acctccagca gaggtaaaac caatgacaaa
     6721 agcaaatatt atcaccacat aaaatgtggg atgcaggcag acggtaaaca gagcaataaa
     6781 tgagaaaaag gtgtacagca tcagtaatgt tgttgggcga acggtattac gaatgagtgg
     6841 agcggtaata aatacacaaa gcagcgaacc cacggtgtag atactgagta gtttaatcga
     6901 catagtgtat gacatgcctg caacaaattg tccgtactgt gccagccact ggctaaccag
     6961 ataaaacgtt gccattgaga tatagccata taaggtataa ctggctaaat cgataattga
     7021 acagcgatgt tccgtagagc tggtggtttt ctttatgaca ggtaagcgac gacccggatg
     7081 gggtgggaac gtacaacggt ataaaaacag agcgttaata aacataatgc ctgcagcaat
     7141 cataaaggac caaccgaacc acagttcagc ccacactaac aggctaatga ttagcggtaa
     7201 taaaaattgt ccgctggaaa caaatgcttt aattaaaata ttggctgtcc caggtgagcg
     7261 tggaaaagct tccatcaaac tgggataagt gcctgcatcg agaaaactgt ttgccattcc
     7321 cgccagaaag ccaaaaacat aggcgataat gatgttattg gtgtgcagga tgccaaaaaa
     7381 gaaggccata tagcagcaca tcccgagcat gataaaaggg cggcgaccaa agcgatcgga
     7441 taataatcct gcaaaaagca agacacttaa tcgaccaatg cccagcgatg agataactat
     7501 cgagacaccc gcggcattag tctgccaaag tgtctccagc gaggccatat tcaggctcat
     7561 caaaatgacg cccataccat gcaccaggta attaaaatac aacccaagtg cggtagggta
     7621 atagggattt ttcataggtt ctacttagca aaggtgcttt aaaaagtgta gcgatgttgt
     7681 gtcagtatcc gttcattatt gttgtaacaa cgacaattcc tttttgtgga gaaattaaca
     7741 aagccgctcc agatcgtgtg catataattc agcggctacc gattgataga tcttccagtc
     7801 aataaaattg ccatcagggt agacttgttg aaacgcggtt aagtcgggga aaaaacgcat
     7861 ggtgttgttg ccgatgcggt tatttatttt ctctatgatg gcatgaagat gtttttttcg
     7921 ttgctgacgc atagccaaca gttgctcgac tacacgatca ggaatggcgc atttgccatt
     7981 ttcccactga cgccagcttt cactgtcacc ggtctgggca atccaggtcg cgcattcatc
     8041 aatagtcatg gcaaaaatat ggcgaagtgc ctggagttca taagcgttca tcgtgtaatg
     8101 gctgcatgaa ataaacaata aaatataaat aacattatca gcctgctgac ggcttgaaga
     8161 gagtcacagt atcttgtgca acattattgg aaagagtgag gggcgaggta gcgaagcgga
     8221 aaaatgttaa aaaaaagccc atcgtgggag atgggcaaag actacacaca gcaattcgtt
     8281 gtttcactca ggggatttcc atgcttataa atcaatatgt tgatttataa ccgtaagcta
     8341 atagtaggca tacggacttt ttgcgtcgat cagattcgtc tcattagttt aaaaactgta
     8401 aagaatttgc gacaggtact accagctgaa tggcggtaag caggaaaata tggggaagag
     8461 taagcaaata tcaattagcg ggggttaaca atcaggcggc agagccgcct gatgagaaat
     8521 tacttattcg gtttctcgat ttcgccaagt tcttcgagaa tttcttccgg ttggtccgtc
     8581 ggcggcggga cttcttccac ccacgcggca aacagacgcc aggaaacggc taacagaacc
     8641 ggaccaataa acagaccgat catcccgaaa gcaatcaaac caccaataac accagagagg
     8701 atcaggatca gcggtaaatc ggcacccatg cgaattaaca ttgggcggat gacgttatcc
     8761 agcgtgccaa ccacaccgct ccacactaac aataccgttc cccaggtggt atcgccagtc
     8821 cagtagagcc agataatcgc cggaatcagt accggcaacg ggccaagctg gacaaggcag
     8881 gagaggatca ttaacaccgt tagcaacgtt gcataaggta cgccggatac ggcgaggccg
     8941 ataccgccaa gcactgcctg tactaacgcc gtcaccacca cacccagcgc caccgcgcgg
     9001 atagcctgtg ccgccagcag tacggcggca tctccgcgaa cgcctgccag acgggttgca
     9061 aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt acagcagggc actgaagaga
     9121 agcatcagcg cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc aacgaaccag
     9181 gtggtggtgg tgccaatata agggcggact ttcgccatga tcgccgtccc ccccatatcc
     9241 agcaagttgt gccagcctgc atacagcttc gcgccaatca ccggaatggt attaagccac
     9301 gccagatcgg gtaacgtcat gtcgccgctg gaaatggctt taattagcgg gccgctgccg
     9361 tcgacgatac tgttaaccag caaagcaata gggatgataa acaccatcac taataacagc
     9421 gtcatcacca gaacggcgag agagcggcgg ccaaacatga tcttttgcaa acgtaacaat
     9481 accggccagg tggcgataac caccgtaccg gcccatgcaa agccgagaat aaagggttga
     9541 acaatccaca gacatgccac aatcatgatg gctaaaaaca gcaccgaaag cagaatttgt
     9601 gcgacatccc tgggctgacg aacatttacc ataactactt ttcacctttt ttgtgcgtca
     9661 aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc aatttcagcg
     9721 gtttttaaca ggccgattct gcccttaatt ctgatgggcg tgttagaaaa aaatgtggta
     9781 caacaactca ggcaacacgc aaacgattta ctcgtcgtat ttcaagttgc atgtgctgcg
     9841 gctacgttcg ttcaccccag tcacttacta ttgtaagctc ctggagttca ctcgcttgcc
     9901 gccttcctgc aactcgaaat ccatagagta aataaacatc acctttcagt gcgctattgc
     9961 agccgcctga aagaatagtg tatcgaccta catcacagac cgcaggaaag ggtcaatata
    10021 atgattccac agatttccca ggcacccggc gtcgttcaac tggtgcttaa ttttttgcaa
    10081 gagctggagc aacaaggttt taccggcgat acggcgacaa gttatgccga tcgtctgaca
    10141 atgtcgaccg acaacagtat ttaccaactt ctccccgatg cggtggtatt tccgcgttca
    10201 accgcagatg tggcgctgat cgcccgtctt gccgcgcagg aacgctattc atcgctgatc
    10261 tttacccccc gtggcggcgg caccggcact aacggtcagg cgctcaacca ggggattatt
    10321 gttgatatgt cccgccatat gaaccgcatc atcgaaatta accctgaaga gggctgggtg
    10381 cgcgttgagg ccggggtgat aaaagatcaa ctcaatcagt acctgaaacc gttcggctac
    10441 ttttttgcgc cggaactttc gaccagcaac cgggcaacgc tcggtgggat gatcaatacc
    10501 gatgcttctg gtcagggatc gctggtctat ggcaaaacgt cggatcacgt acttggcgta
    10561 cgtgcggtgt tgttgggggg cgatattctc gatacgcaac ctttacccgt cgaattggcg
    10621 gaaacgctgg gtaaatccaa taccacaatc gggcgaattt ataacacggt ttatcaacgt
    10681 tgccgtcagc aacgccagtt aatcatcgac aacttcccca aacttaaccg ctttcttacc
    10741 ggttacgatc tgcgtcatgt ctttaacgat gagatgaccg agttcgacct gacgcgcatt
    10801 ctgacgggtt cagaagggac gctggccttt attaccgaag cgcggctgga tattacgcgc
    10861 ttgcctaaag tgcgccgtct ggtgaatgtc aaatatgact cttttgactc tgcgctgcgt
    10921 aacgcgccgt ttatggttga ggcgcgggcg ctttcggtag agacggtgga ctcaaaagtg
    10981 ctgaatctgg cgcgggaaga tattgtctgg cattccgtca gcgagttgat taccgatgtg
    11041 cctgacaaag agatgctcgg actgaacatt gtggaatttg ctggtgatga tgaggcgctg
    11101 attgacgagc gagttaatgc actctgtgtg cggcttgatg agctgattgt cagccaacag
    11161 gcaggtgtga ttggctggca ggtgtgccgc gagctggcgg gtgttgaacg tatctatgcg
    11221 atgcgcaaaa aagccgttgg tctgcttggc aatgccaaag gtgccgctaa gccaattccg
    11281 tttgctgagg atacctgtgt accgccggaa catctggcgg attatattgc tgaatttcgc
    11341 gcgctgctcg acagccacgg cttaagctac ggtatgttcg gtcacgtcga cgcaggtgtt
    11401 ttgcacgtcc gtccagcgct ggatatgtgc gatcctcaac aagagatttt gatgaagcaa
    11461 atctctgatg acgtggtggc gctgactgcg aaatacggtg gtttgttgtg gggcgagcac
    11521 ggcaaaggtt ttcgcgctga atacagcccg gcgtttttcg gtgaggaact ttttgcagaa
    11581 ctgcgcaaag tgaaagcggc atttgacccg cataaccgac tcaacccagg gaagatttgc
    11641 ccgccagaag gtctcgatgc gccgatgatg aaagtggacg cggtgaagcg cggtacattc
    11701 gatcggcaga tctccattgc ggtacgccag cagtggcgcg gtgcgatgga gtgtaacggc
    11761 aacggtttat gcttcaactt tgatgcccgt agtccgatgt gtccgtcgat gaagatcacc
    11821 cagaaccgga ttcattcacc gaaagggcgc gcaacgctgg tgcgtgaatg gctgcgtttg
    11881 ttggcggatc gcggcgttga tccactcaaa ctggaacaag aactgcctga atccggcgtc
    11941 agtttgcgga cgttaattgc ccgcacgcgc aatagctggc atgcgaataa aggcgaatat
    12001 gacttctcac acgaagtcaa agaggcgatg tcgggctgtc tggcctgtaa agcgtgttcg
    12061 acccagtgcc ccatcaaaat tgatgtgccg gagtttcgct ctcgttttct gcagctctat
    12121 cacacccgtt atttacgccc gctgcgcgac cacctcgtcg ctacggtcga gagctacgcg
    12181 ccgctgatgg cacgcgcgcc gaaaaccttt aacttcttca ttaaccagcc gctggtgcgc
    12241 aaactctcgg aaaaacatat cggcatggtt gatttgccgc tgttgtcggt cccctcgcta
    12301 caacaacaaa tggtggggca tcgctcggca aacatgacgc tggaacagct tgaatctctc
    12361 aatgcagagc agaaagcgcg cacagtgttg gtggtgcagg acccctttac cagctattac
    12421 gatgcgcaag tggtggcgga ttttgtccgt ctggtcgaaa aattaggttt ccagcctgtg
    12481 ttactgccat tttcgccaaa tggcaaagcc cagcatatta aaggttttct taatcgtttt
    12541 gcgaagacgg cgaaaaagac ggcggatttc ctcaaccgta tggcgaagct gggtatgcca
    12601 atggtgggcg tcgatccggc gctggtactt tgttatcgcg atgaatataa actggcgctg
    12661 ggcgaggaac gtggcgagtt taacgtctta ctggcgaatg aatggctggc aagcgcactt
    12721 gagtcacagc cagtggctac agtcagcggt gaatcatggt atttctttgg tcactgtacc
    12781 gaagttaccg ccttgccggg tgcgccagca caatgggccg cgatatttgc ccgttttggc
    12841 gcgaaactgg aaaatgtcag cgtgggttgc tgcggcatgg cagggactta cggacatgaa
    12901 gcgaaaaacc ataaaaattc gctcgggatc tatgagttat cctggcatca ggctatgcag
    12961 cgactgccgc gtaaccgctg tctggcgacc ggatattcct gccgtagcca ggtaaaacgg
    13021 gttgaaggca cgggggtacg ccatcctgtg caggctttac tggagattat taaatgatat
    13081 ggaaacggaa aatcaccctg gaagcactga atgctatggg tgaaggaaac atggtggggt
    13141 tcctggatat tcgctttgaa catattggtg atgacaccct tgaagcgaca atgccagtag
    13201 actcgcggac aaagcagcct ttcgggttgc tgcatggagg agcatccgtg gtactggccg
    13261 aaagtatcgg ttccgttgcc ggttatttat gtaccgaagg tgagcaaaaa gtggttggtc
    13321 tggaaatcaa tgctaaccac gtccgctcgg cacgagaagg gcgggtgcgc ggggtatgca
    13381 aaccgttgca tctcggttcg cgtcaccagg tctggcagat tgaaatcttc gatgagaaag
    13441 ggcgtttgtg ctgttcgtca cgattgacga ccgccatttt gtgacgttgt caccagaaaa
    13501 gtgtgacgca tattgccttt ggtcattatc tgcacagtaa acggtggtat actgtgcagg
    13561 tcttgtctaa aagcgaggat gctatgtcta ctcaacttga tcccacccag ctggccattg
    13621 aatttttacg ccgtgaccag agcaatcttt cacctgccca atatctcaaa cgtctgaaac
    13681 aactagaact tgaatttgct gacctgctta ctctctcttc tgctgaatta aaagaagaaa
    13741 tctactttgc ctggcgtctg ggcgtgcatt gatcctgact taatgccgca agttcgccaa
    13801 atcgacctgg attaacctcc gtccaggttc gatgaccttc ttaatactgc ttatctaccg
    13861 cctgatattg ttcaaaaata atactaatcg cagtttgtgt taaaacggcg ggttagcttt
    13921 atgagattat tcttatcgcc ccttcaagag ctaagccact gagagtgccg gagataagcg
    13981 ccggatgggg tagaaaccct taagcctgtg ttgcacagac ttaagggttt tcttatttct
    14041 gaacgctgca cttttttacc tatctcttaa atagtcattt tcaatacaac tttttctttt
    14101 tcatcacagc cttaatgctc cttagataat aaccattatc taacaatgag atacctaatt
    14161 cttagcgtgc ctgttaaccc acacatcagg gtctatgctt attaaaagca ggcaaaaagc
    14221 atgttttacg gtaaagcccc tgcgtttgct tggttgaact gataatcatt atcactaaca
    14281 tgctgttata cgctgaaagc gatgaagtga ggtaaatcga tggatatgca ttcaggaacc
    14341 tttaacccac aagatttcgc ctggcaaggc ttaacgctga cacccgcagc ggcgatacac
    14401 atccgtgagc tggtggcaaa gcagccgggt atggtcggcg tgcgcttagg cgtgaagcaa
    14461 acgggctgcg cgggctttgg ctatgtgctc gacagtgtta gcgagccgga caaagacgat
    14521 ctgctgtttg aacacgacgg cgcgaagctg tttgtcccgc tgcaagcgat gccgtttatt
    14581 gatggcacgg aagtcgattt cgttcgtgaa ggacttaatc agatattcaa atttcacaac
    14641 cctaaagccc agaatgaatg tggctgtggc gaaagctttg gggtataggc ggtactatgt
    14701 ctcgtaatac tgaagcaact gacgatgtca aaacctggac cggcggcccg ctgaattata
    14761 aagaaggatt cttcacccag ttagccaccg atgagctggc aaaggggata aacgaagagg
    14821 tggtgcgcgc aatttcggcg aagcgtaatg agccggagtg gatgctggag tttcgtctaa
    14881 acgcctatcg cgcatggctg gagatggaag aaccgcactg gttgaaagcg cactacgaca
    14941 agctgaatta tcaggattac agctactact cagcaccatc gtgcggtaat tgtgacgaca
    15001 cttgcgcgtc tgaacctggc gcggtgcagc aaactggcgc gaacgccttt ttaagtaaag
    15061 aggtggaggc ggcgtttgag cagttgggcg ttcccgtgcg ggaaggcaaa gaggtggcgg
    15121 tggatgccat tttcgactca gtttcggttg ccactactta tcgcgaaaaa ctggcggagc
    15181 agggaattat tttctgttcc tttggtgagg cgatccacga tcacccggaa ctggtgcgta
    15241 aatatctcgg caccgtggtg ccggggaatg acaacttctt tgccgcgctt aatgcggcgg
    15301 tagcctctga cggtacgttt atttatgtgc ctaaaggcgt gcgctgcccg atggaacttt
    15361 ccacctattt tcgcattaac gcggaaaaaa ccgggcagtt tgagcgtacc attctggtgg
    15421 ccgacgaaga cagctacgtc agctacattg aaggctgttc cgctccggtg cgtgacagct
    15481 atcagttaca cgcggcagtg gtggaagtca tcatccataa aaacgccgag gtgaaatatt
    15541 ccacggtaca aaactggttc cctggcgata acaacaccgg cggtattctc aacttcgtca
    15601 ccaagcgtgc tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa tcagaaaccg
    15661 ggtccgcgat tacgtggaaa tatcccagct gcattttgcg cggcgataac tccattggtg
    15721 agttttactc agtggcgctg accagcggtc atcagcaagc ggataccggc accaagatga
    15781 tccacatcgg taaaaacacc aaatcgacca ttatctcgaa agggatctct gccggacata
    15841 gtcagaacag ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat gcgcgcaatt
    15901 tcactcagtg cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc ttcccgtatg
    15961 tcgaatgtcg caataacagc gcacaactgg agcacgaggc aacgacatca cgtattggtg
    16021 aagatcaact gttttactgc ctgcaacgcg ggatcagcga agaagacgcc atctcgatga
    16081 ttgttaacgg tttctgcaaa gacgtgttct cagaactgcc gctggaattt gccgttgaag
    16141 cacaaaaact cctcgccatc agtcttgaac acagcgtcgg ataaggaata aacatgttaa
    16201 gtattaaaga tttacacgtc agcgtggaag gtaaagctat cctgcgtgga ttaagcctcg
    16261 ccgttcgtcc cggcgaagtt cacgccatta tggggccaaa cggttcgggc aaaagtacct
    16321 tatcggcaac gcttgccggg cgcgaagatt atgaagtgac gggcggcacg gttgagttca
    16381 aaggcaaaga tttgcttgca ctgtcgccgg aagatcgtgc aggcgaaggc atctttatgg
    16441 ccttccagta tccggtggag attccaggcg tcagtaacca gtttttcctg caaacggcac
    16501 ttaatgcagt gcgcagctat cgcggccagg aaacgctcga ccgctttgat tttcaggatt
    16561 tgatggaaga gaaaatcgct ctcctgaaga tgccggaaga tttattaacc cgttcggtaa
    16621 acgttggttt ttccggcggc gagaaaaagc gcaacgatat tttgcaaatg gcggtgctgg
    16681 aaccggagtt atgcattctt gatgagtcgg actccgggct ggatattgac gcattaaaag
    16741 tggtcgccga tggcgtgaac tcgctgcgtg atggcaagcg ctcattcatc attgttacgc
    16801 actaccaacg cattctcgac tacatcaagc ctgattacgt ccatgtgcta tatcagggac
    16861 gaattgtgaa atccggcgat ttcacgttgg tcaaacaact ggaggagcag ggttatggct
    16921 ggcttaccga acagcagtaa cgcgctgcaa cagtggcatc acttgtttga agctgaaggg
    16981 acaaaacgct caccgcaagc acagcagcat ttacaacaat tgctgcgtac cggactgccg
    17041 acacgtaaac atgaaaactg gaaatatacg ccgctggaag ggctgaccaa tagccagttt
    17101 gtcagcattg cgggagagat atccccacag cagcgagatg ccttagcgtt aacgttagac
    17161 gctgtgcggc tggtatttgt cgatggacgt tacgtgtcag cactgagcga tgcgactgaa
    17221 ggcagcgggt atgaagtgag cattaacgac gaccgtcagg gggtacccga cgctattcag
    17281 gcggaagtgt ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca tatcgccgtg
    17341 aagcgcggtc aacggccggc aaagccattt ctgttaatgc atattaccca gggcgtggca
    17401 ggtgaagagg tcaacactgc ccattaccga catcatctgg atctggcgga aggtgccgaa
    17461 gcaacggtga tcgaacattt tgtcagcctt aatgatgctc gtcacttcac cggcgcacgg
    17521 ttcactatca atgtcgcagc gaacgcccac ttgcagcata tcaagctggc gtttgaaaac
    17581 ccggtcagtc accactttgc ccataacgat ttgttgctgg ctgacgatgc caccgcattt
    17641 agccatagtt tcctgctggg tggcgcagtg ttacgacaca acaccagtac gcaactcaat
    17701 ggcgaaaaca gcacgctgcg gatcaatagc ctggcgatgc cggtgaaaaa cgaggtgtgt
    17761 gatacccgta cctggctgga acacaataaa ggtttttgta acagccgaca gttgcacaaa
    17821 actatcgtca gcgacaaagg ccgcgcggta tttaacggtt tgatcaacgt cgcgcagcac
    17881 gccatcaaaa cggatggtca gatgaccaac aataatctgc tgatgggcaa actggcagaa
    17941 gtggatacga aaccgcagct ggaaatctat gcagatgatg tgaaatgcag ccacggcgcg
    18001 acggtggggc gtattgatga tgaacagatg ttctatctgc gctcgcgcgg gatcaatcag
    18061 caggatgccc agcagatgat catttacgcc tttgctgccg aactgacgga agcactgcgt
    18121 gatgaggggc ttaaacagca ggtgctggcc cgaatcggtc aacgactgcc aggaggtgca
    18181 agatgacttt ttccgtcgac aaagtgcggg ccgactttcc ggtgctttcg cgtgaggtaa
    18241 acggtttgcc gctggcttat ctcgacagcg ccgccagtgc gcagaaacca agccaggtga
    18301 ttgactccga agccgagttt tatcgtcacg gctacgcggc ggtgcatcgc ggtattcata
    18361 ccttaagcgc ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca tcgctgttta
    18421 ttaatgcccg ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa gggatcaatc
    18481 tggtcgccaa tagttggggc aacagcaacg tgcgggcggg cgataacatc atcatcagtc
    18541 agatggagca ccacgctaac attgttccct ggcagatgct ttgcgcacgc gttggcgcag
    18601 agctgcgtgt gatcccgctc aatcccgacg gtacgctgca actggagacg ctacctaatc
    18661 tgttcgatga gaaaactcgc ctgctggcaa ttactcatgt ctccaacgtg cttggcacag
    18721 aaaatcccct ggcggaaatg atcacgcttg cgcatcagca tggcgcaaaa gtgctggtgg
    18781 atggcgctca ggcggtgatg catcatccgg tggacgttca ggcgctggat tgcgattttt
    18841 acgtattctc cgggcataaa ctgtatggcc ccaccggaat tggcattctt tatgtgaaag
    18901 aagccttgtt gcaggagatg ccgccgtggg aagggggcgg ttctatgatc gccaccgtca
    18961 gcctgagtga aggcactacc tggaccaaag caccatggcg gtttgaagcc ggtacaccca
    19021 ataccggggg catcattggt cttggcgcgg cgctggagta tgtttcggcg ctggggctta
    19081 atagcatagc cgagtatgaa cagaatctga tgcactacgc gctatcacag ctggaatctg
    19141 taccggatct cactctctat ggcccgcgga acaggcttgg cgttattgct tttaatcttg
    19201 gtaaacacca cgcctatgat gttggcagtt ttctcgataa ctacggcatt gctgtgcgta
    19261 ccggacatca ctgcgctatg ccattaatgg cctattacaa cgtccctgct atgtgtcggg
    19321 cgtcgctggc catgtataac acccatgaag aagtggatcg tctggtgacc ggactgcaac
    19381 gtattcaccg tctgctggga taacagggag gcactatggc tttattgccg gataaagaaa
    19441 aattgctgcg taatttttta cgctgcgcca actgggaaga gaaatatctc tacattattg
    19501 agctgggcca gcgtctgcca gaattacgcg ccgaagaccg aagcccacaa aatagcattc
    19561 agggctgcca gagtcaggtg tggattgtca tgcgccagaa tgcgcaggga attattgaat
    19621 tgcagggcga cagcgatgcg gcgattgtga aagggcttat tgcggtcgtc tttattctct
    19681 acgatcagat gacgccgcag gatattgtca atttcgatgt gcgtccgtgg tttgaaaaaa
    19741 tggcgctcac ccaacatctc accccatctc gttcacaagg tctggaagcg atgattcgcg
    19801 caattcgcgc caaagccgct gcacttagct aaactaaagg gacagctttc atccggttgg
    19861 ctcacattgc cggatggtca atgtttacac tgacctgcac aggagtgttt tggtttcagg
    19921 tgaacataag cgccatccga cgtttcagcg tgagtctccg gcaaatacag gaggtttaca
    19981 atgaaacgcg cgtctttgct tacactcacg cttatcggcg cttttagcgc catccaggct
    20041 gcctgggcgg ttgattatcc gctaccacca accggaagcc gactggttgg gcaaaatcaa
    20101 acgtatacgg tgcaagaagg ggataaaaac cttcaggcta ttgcccgacg ttttgatact
    20161 gcggcaatgt tgatccttga agccaataac actatcgccc cggtgccaaa acctggtacg
    20221 acgataacta ttccttcaca actgttatta cctgatgcac cgcgtcaggg gattatcgtt
    20281 aaccttgcag agctgcgcct ttattattat ccgccgggag aaaatattgt gcaggtttat
    20341 ccaataggta ttggattgca ggggctggaa acgccggtga tggaaacgcg tgttgggcag
    20401 aaaatcccta acccaacctg gacgcctacg gcaggcattc gtcagcgttc gctggagcgt
    20461 ggcattaaat taccgccagt cgttcctgcc ggaccaaata acccgctagg acgttacgca
    20521 ctgcgcctcg cgcatggtaa tggcgaatac ctcattcatg gcaccagtgc gccggacagc
    20581 gttggtttgc gcgtcagttc agggtgtatt cgcatgaatg caccggatat taaagccttg
    20641 ttctccagcg tgcggacggg aacgccggtg aaagtgatca acgaaccggt gaaatattcc
    20701 gtggaaccta acgggatgcg ttatgttgaa gtacatcgac cactatcagc agaagaacag
    20761 caaaacgttc agacaatgcc atacacactg ccagcaggct ttacgcaatt taaagacaat
    20821 aaggctgtag atcagaagtt agtcgataaa gcgttgtatc gtcgggcagg gtatccggtt
    20881 tcggtgagca gtggagcaac tcccgcagcc agcaatgcgc cttcagtaga gtcagcgcag
    20941 aatggtgaac cagagcaagg gaatatgtta cgcgcgacgc agtagcggta aacggcagac
    21001 aaaaaaaatg gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta
    21061 tttagtagcc atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg
    21121 aacgtcggaa cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt
    21181 ctgaacgtca gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt
    21241 agaacccagg attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta
    21301 gattgagtta atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat
    21361 atttttttga tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg
    21421 ggttttagat aaaaaaattg agagaaa
//
LOCUS       DAZFWP020000081        21236 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFWP020000081 DAZFWP020000000
VERSION     DAZFWP020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.081"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            104..799
                     /gene="yafL"
                     /locus_tag="ACHK0Z_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     CDS             104..799
                     /gene="yafL"
                     /locus_tag="ACHK0Z_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4502884.1"
                     /translation="MPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQASY
                     IVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITE
                     VAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATI
                     VANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGEIIRVSRLAEPFWQDHF
                     LGARRILTEETIL"
     gene            complement(856..1212)
                     /locus_tag="ACHK0Z_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             complement(856..1212)
                     /locus_tag="ACHK0Z_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4502885.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(1205..1483)
                     /locus_tag="ACHK0Z_RS14485"
                     /old_locus_tag="pgaptmp_002897"
     CDS             complement(1205..1483)
                     /locus_tag="ACHK0Z_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4502886.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(1588..3300)
                     /locus_tag="ACHK0Z_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /pseudo
     CDS             complement(1588..3300)
                     /locus_tag="ACHK0Z_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3272..4057
                     /gene="lafU"
                     /locus_tag="ACHK0Z_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             3272..4057
                     /gene="lafU"
                     /locus_tag="ACHK0Z_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4502887.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPKSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            4128..5183
                     /gene="dinB"
                     /locus_tag="ACHK0Z_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             4128..5183
                     /gene="dinB"
                     /locus_tag="ACHK0Z_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4502888.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            5180..5632
                     /gene="yafP"
                     /locus_tag="ACHK0Z_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             5180..5632
                     /gene="yafP"
                     /locus_tag="ACHK0Z_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4502889.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            5878..7017
                     /locus_tag="ACHK0Z_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     CDS             5878..7017
                     /locus_tag="ACHK0Z_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4502890.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            7014..7628
                     /gene="prfH"
                     /locus_tag="ACHK0Z_RS14515"
                     /old_locus_tag="pgaptmp_002903"
     CDS             7014..7628
                     /gene="prfH"
                     /locus_tag="ACHK0Z_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4502891.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(7685..9142)
                     /gene="pepD"
                     /locus_tag="ACHK0Z_RS14520"
                     /old_locus_tag="pgaptmp_002904"
     CDS             complement(7685..9142)
                     /gene="pepD"
                     /locus_tag="ACHK0Z_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4502892.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            9403..9861
                     /gene="gpt"
                     /locus_tag="ACHK0Z_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             9403..9861
                     /gene="gpt"
                     /locus_tag="ACHK0Z_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4502893.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            9953..11197
                     /gene="frsA"
                     /locus_tag="ACHK0Z_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             9953..11197
                     /gene="frsA"
                     /locus_tag="ACHK0Z_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4502894.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            11255..11656
                     /gene="crl"
                     /locus_tag="ACHK0Z_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             11255..11656
                     /gene="crl"
                     /locus_tag="ACHK0Z_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4502895.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(11695..12750)
                     /gene="phoE"
                     /locus_tag="ACHK0Z_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             complement(11695..12750)
                     /gene="phoE"
                     /locus_tag="ACHK0Z_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4502896.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            13038..14141
                     /gene="proB"
                     /locus_tag="ACHK0Z_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             13038..14141
                     /gene="proB"
                     /locus_tag="ACHK0Z_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4502897.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            14153..15406
                     /gene="proA"
                     /locus_tag="ACHK0Z_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             14153..15406
                     /gene="proA"
                     /locus_tag="ACHK0Z_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4502898.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            15521..15596
                     /locus_tag="ACHK0Z_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     tRNA            15521..15596
                     /locus_tag="ACHK0Z_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15554..15556,aa:Thr,seq:cgt)
     gene            complement(15723..16061)
                     /locus_tag="ACHK0Z_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /pseudo
     CDS             complement(15723..16061)
                     /locus_tag="ACHK0Z_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(16112..17068)
                     /gene="paoD"
                     /locus_tag="ACHK0Z_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             complement(16112..17068)
                     /gene="paoD"
                     /locus_tag="ACHK0Z_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGU4502899.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHLTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLETQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(17078..19276)
                     /gene="paoC"
                     /locus_tag="ACHK0Z_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             complement(17078..19276)
                     /gene="paoC"
                     /locus_tag="ACHK0Z_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU4502900.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            complement(19273..20229)
                     /gene="paoB"
                     /locus_tag="ACHK0Z_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             complement(19273..20229)
                     /gene="paoB"
                     /locus_tag="ACHK0Z_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGU4502901.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(20226..20915)
                     /gene="paoA"
                     /locus_tag="ACHK0Z_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             complement(20226..20915)
                     /gene="paoA"
                     /locus_tag="ACHK0Z_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGU4502902.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
BASE COUNT         4915 a         5554 c         5709 g         5058 t
ORIGIN      
        1 cgggccggga tacactgcaa catcaatgca tgatcagcca ttattgagta tgtccttgcc
       61 gtcgattccc tcttttgtgt tgtcgggatt actgttgctt tgcttgccgt tttcttcatt
      121 tgccagcgcc accacatcac atatcacttt cagctacgcc gcccgccagc ggatgcaaaa
      181 tcgtgcgcgt ttattaaaac agtaccaaac ccatctgaaa aaacaggcca gctatattgt
      241 ggaaggcaat gccgaaagca gaagggcgct acgccagcac aaccgcgaac agataaaaca
      301 gcatccagaa tggtttcctg cgccgctcaa ggcgagtgac agacgctggc aggcgctggc
      361 ggaaaacaac catttcttaa gcagcgacca tctgcataac atcaccgaag tggcgattca
      421 ccgtctggag caacagcttg gcaagcctta cgtctggggc ggtacgcggc ccgatcaagg
      481 cttcgactgt agcgggttag ttttttatgc ctacaacaag atccttgagg cgaaactccc
      541 acgcacggct aatgagatgt atcactatcg ccgggcaacc attgtggcga ataacgacct
      601 gcgccgggga gatttgctat ttttccatat ccacagccgc gagattgccg accatatggg
      661 ggtgtatctg ggcgacggtc agtttattga gtcaccgcgc accggggaaa tcattcgggt
      721 aagtcggtta gctgaacctt tctggcagga tcacttttta ggcgcgcggc ggatattgac
      781 ggaagagacg attttgtaaa tgggtaatat tgacgaactg gcaaaaggtc ggatgccacg
      841 cggcatccga cctgtttaca tctgggctga aaactcacgt tctaagacat caacaatgag
      901 ctggtttttt gaaatggaat gttgtctggc gacgatggtc agccgcgaat caatgtgatg
      961 aggaacacga agcgtcaggc gtttttgctc ttcttcttca cgaaaagggg caataccgtc
     1021 ctcttcacaa tccgccatcc actcatccag cgcgctttcg ccttcacgat ataatgcctc
     1081 aatacttgtg gctacaaagt cacaataacc gtttacattg aggaacttgc cgcgaaatgc
     1141 gttgatatcg gcttcaaatg tcactgcggc tggttgacct ttaattgtga taatgttcga
     1201 tgctttattc ataatctacc cctacacagt taacaaacca ctccttaaga cctgctactg
     1261 cgcctttatc catcacattc tttggatgtg gctcatgtgt gctgaatttg gtcgttccaa
     1321 tctgaaattt tcgtctggag ccgttgttat tttttattgt tccacccaca gctttaatga
     1381 gtgcaacaac atcatcccat ttaattccag attttggcgg ctccgtcatt acatcgtcga
     1441 atgtttttct atgacgagaa cctagcgcgc tgaccttaat catactatca atccatgacg
     1501 tcattgtatg gtgtcatcgt agtctctttg cagtgtgacg tcaattaatg gtgtcaggtg
     1561 ctgcaaagca tggaaaagaa tcccccatca actcaacgcc cccataatct tcaactccag
     1621 ctcatccggc acttcgttat acgacagcac atgcagcccc ggcgcaaaca accttgcata
     1681 acgcgccagc aaagggcgca gctgcggtgg caccagcagc accgggtctt tccccgccgc
     1741 tttcatctgc tctttcacct gtggcatcgt gctctggaac tggttgagca tattcgggtc
     1801 cactggcacg ctgtcgagca tcactttacc gccctgttgc gcctgattca ctaggttagt
     1861 cagcagattt tccagctcat tattcagggt atacaccgtc agctcctgct tgcgaacgaa
     1921 cggatgggta atgctgcgcc gcagcgccag gcgcacatcg gctgccagca gaatatgatc
     1981 tttggtcacc gcgctactgg cgaccagcac ggtggcgatg gtgacgatat cgcgcaggga
     2041 aacgccttcg gtcagcagcg cccggtacac tttcagcaac tggctgtaat tgagcaccgc
     2101 gcttaaatcc tccgccaggc gcggtgccat cgacgccaaa cggttatgca actgcgtaat
     2161 atcgtcatag ttaaataaat caggaatata gctgcgcaca atcttattta catgggtagc
     2221 aatcacgctg gcgctgtcga tcacctgata ccccatattc agcgccttcg ctttctgcgc
     2281 aggctggatc caggtcaccg gcatcccgta cgccgggtcg ttccccagca cgccgtctat
     2341 ctcgccgtag gtttcgctgg agggcagcgc catcagttta tccgccggaa tatccgcttc
     2401 atcagcctta atgccgttga tgaaaatagc gtactggctg ggcttaaggc ggaagttttc
     2461 ccgaatgcgg atctccggca gcagcacgcc gttgccgtcg gatatcacct gtcgcacgcc
     2521 gcgaatacgc tgggtgagcg ggttgccctg ggctttgtcc accagcgcca ccagcttgta
     2581 gccgaggctt aagctgatgg gctcgatcag cggaatggtt tcccaactga cctgttgctc
     2641 gctggtttcg gtgatggtgc gggtcagcgt ttcgagactt ttctcttccg cttccgccgc
     2701 ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc agggcgctga acagcaaaaa
     2761 cggcagatgt ggcattcccg gcactaccgc cagtacgaac ataattccgg tagcggtgta
     2821 gagcaccgat gggcttgcca gcaactggtt acgcacgtca tgggcgatat cgccgttgtc
     2881 gctgacgcgg gtgacgataa tcgccgccgc ggtggagagt agcagggaag ggatttgcgc
     2941 caccagcccg tcgccgatgg tcatcagcac atactgctgg aaggcggcgt cggcgctcag
     3001 gttgtatttg aagatcccga tacagacgcc gccgatcagg ttgatcgcca gaatcatcat
     3061 accggcgata gcatccccgc gcacaaattt cgatgccccg tccatcgcgc cgtagaaatc
     3121 ggcctcgctg gcaacatctt tacgtcgggt ttgtgcctgc gcctgattga tcaacccggc
     3181 attaagatcg gcgtcaatcg ccatctgctt accgggcatc gcgtccaggg taaagcgggc
     3241 agaaacctcg gagatacgct cggccccttt ggtgacgaca ataaagttga ttgtcaacag
     3301 cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg cacgggcagt cgatttttaa
     3361 cggtggtggg ctgtcgccgt taaataaaat cagcccgtct catccgccaa aacctgccac
     3421 cgtggcggtg ccggaagaaa cggagaaaaa agcgcgcgac gtcaatgaga aaacggcgct
     3481 gctgaagaag aaatccgcca ccgagcttgg cgagctggca accagtatca ccaccattgc
     3541 ccgtgatgcg catatggaag cgaatctgga gatggagatt gttccccagg gattacgcgt
     3601 gctgattaaa gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc agattatgcc
     3661 gttctttaaa acgctgctgg tggagctcgc gccagttttc gactcgctcg ataacaaaat
     3721 tattattacc gggcataccg atgcgatggc ttacaaaaac aatatctaca acaactggaa
     3781 cctttcgggt gaccgcgcgc tttcggctcg tcgggtgctg gaagaggccg gaatgccgga
     3841 agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggatt ccaaaaatcc
     3901 gaaaagcgcg ggcaaccggc gcattgagat tatggtgctg accaaaagtg cgtccgatac
     3961 gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg tgcaaaagct
     4021 ggataagcag caggtgcttt cgcagcgaac gcgttaaatg ctgaatcttt acgcatttct
     4081 caaaccctga aatcactgta tactttacca gtgttgagag gtgagtaatg cgtaaaatca
     4141 ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat cccgccctgc
     4201 gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc agcaccgcca
     4261 attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc gacagggatg gcgctcaaat
     4321 tatgcccgca tctcaccttg cttccggggc gctttgacgc ctacaaagaa gcctcaaatc
     4381 atatccgtga aatcttctcg cgctacacct cgcgtattga accgttgtca ctggatgagg
     4441 cttatctcga cgtcaccgat agcgtccatt gccacggttc tgcgaccctc atcgcccagg
     4501 aaatccgcca gacgattttc agcgagctgc aactgacagc gtctgcgggc gtggcaccag
     4561 taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggtcag tttgtgatta
     4621 cgccggcaga agttccggca tttttacaaa ccttaccgct ggcaaaaatc cccggcgtcg
     4681 gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg gacctgcggt gatgtacaaa
     4741 agtgtgatct ggtgatgctg cttaaacgct ttggcaaatt tggccgcatt ttgtgggagc
     4801 gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttgcgaaaa tccgtcggcg
     4861 tggaacgcac gatggcggaa gatatccacc actggtctga atgtgaagcg attatcgagc
     4921 ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa acctgattta ctgattgccc
     4981 gccagggggt gaaattaaag ttcgacgatt ttcagcaaac cactcaggag cacgtctggc
     5041 cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat gaacgccgcg
     5101 gcgggcgcgg tgtgcgactg gtggggctgc atgtgacgtt gcttgacccg caaatggaaa
     5161 gacaactggt gctgggatta tgaataacat acaaataaga gactatcagc ctggcgattt
     5221 tcagcaacta tgcgctattt tcattagagc ggttacgatg actgccagtc agcattattc
     5281 accacaacaa attgccgcct gggcgcagat tgacgaatct cgctggaagg agaaactcgc
     5341 gaaatcacaa gtgcgggttg cagtcattaa tgcaaaactg gtcggcttta ttacctgcgt
     5401 tgaacactat atcgatatgt tatttgttga ccctgaatac acccgccgtg gggttgccag
     5461 cgctttgtta aaacctttga ttaagtctga atccgaactt acggtggacg caagcataac
     5521 cgcaaaaccc ttttttgaac gttatggttt tcagacagtt aagcagcaac gcgttgaatg
     5581 tcggggagag tggtttatta atttttatat gcgttataaa cagcaacatt aaatccagct
     5641 tgcaatgaaa ataacgcaag cctagtatgt gcgcgttttc ttattccaca aactgcaagg
     5701 aggtaaatca tgacaaatcc tttatcaatg actctttgca gacctttcca ggattaattc
     5761 tttttttctt gccctggatt cgtctgccat ttcctgattt tttaattgtt actctcaata
     5821 attcaagtcg caacacatgc aacttgaagt atgacgagtc catttacgga atggtatatg
     5881 ggcaattata ttcgtccctt atccgatgcg gtatttacca tcgcatctga tgacctgtgg
     5941 atcgagagtt tagcgatcca acaattacac accacggcaa atttacccaa catgcagcgc
     6001 gtagtgggga tgccagattt acatcccgga cgcggctatc cgattggcgc agcgttcttc
     6061 tctgttggtc gtttttatcc ggcactggtc ggcaatgata tcggctgcgg tatggcgcta
     6121 tggcaaacag atattctcgc tcgcaaatac aacgccgata agtttgaaaa gcgattatca
     6181 gatctggatg acgttgctga agaaagctgg ctggaggaaa acctgccgtc agcatttgca
     6241 cagcatccgt ggcgcagctc gcttggctct atcggtggcg gcaatcattt cgctgaactg
     6301 caacaagttg atcaaatcat caacgctgaa ctgtttgcac tggcaggtct ggatgcacaa
     6361 catctgcaac tgttggttca tagcggctcg cggggggtgg ggcagtctat tttacagcgg
     6421 catattgcct cgttctcgca tcatggtttg cctcaaggca gtgacgacgc gctacggtat
     6481 attgcggaac atgacgatgc gctggcgttt gcgtgtatta atcgccagat gatcgctttg
     6541 cgcataatgc aacaagttaa ggccacggga agtccggttc tggatgtggc gcataacttt
     6601 gttagcgcgt gtcaaatcgg cgatcaacag ggctggttgc atcgtaaagg tgccacgccg
     6661 gatgacaacg gtctggtgat tattcccggt tcacgtggtg attactcctg gattgtacag
     6721 cctgttgcga acgaaaaaac gctccactcg ctggcgcatg gggcagggcg taaatggggg
     6781 cgcaccgagt gtaaagggcg tctggcagcg aaatacacgg cgacgcaact ctcgcgtact
     6841 gaacttggca gtcgggtaat ttgtcgcgat aaacaactca tctttgaaga agcgccacaa
     6901 gcctataaat cggctgaaag cgtggtgcaa tgtctggtgc aggccggttt aattattcct
     6961 gtcgcgcggc tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa aggatgatct
     7021 tgctacaact ctcctcagcg caggggccgg aagaatgttg tctcgcagtg aaaaaggcgc
     7081 tggacaggct tattaaagaa gctgcccgac aagacgtcgc ggtaacggtg ttggaaacag
     7141 aaacgggccg ctactctgac acgctgcgtt cggcgctgat ttctctggat ggcgataacg
     7201 catgggcatt aagcgaaagc tggtgtggcg ctattcagtg gatttgtccg agtccgtatc
     7261 ggcctcatca tgggcgcaaa aactggtttc tgggcattgg gcgttttacc gctgatgagc
     7321 aggaacaatc ggatgcaatc cgttatgaga cgttgcgttc gtcggggccg ggcggtcaac
     7381 atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt attagcgtga
     7441 aggttcagtc agagcgtagt cagcatgcta acaagcggct ggcacgattg ctgattgcct
     7501 ggaagctgga gcagcagcaa caggaaaata gcgcggtgct gaaatcgcag cggcgaatgt
     7561 tccatcacca gattgaacgt ggcaacccga gacggacatt tacagggatg gcttttatcg
     7621 aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc agcaaatcaa
     7681 ataattactt cgccggaatt tctttcagca gttcagtcag cagcgtccag taatgaccta
     7741 cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt ggcccgatag
     7801 aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga
     7861 taatctggat gttcggtgtt ttgttgaaca gacgctgata ggtttcacgt accagatgca
     7921 tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct tcggttttcg
     7981 cgccagccag tttacccagc gaatccagca tgctcacgac gtagtcttta ccgctgtcga
     8041 tcagtgaacg gatcagacag tgaatttcta cgttattatc agtcatggtc accacaccga
     8101 cgttcaggga ggtttcgacc acaccttttg ccacgtctga gttgcggatc acaccgttcg
     8161 gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg gcagctttat
     8221 cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc agctcgtttt
     8281 tcaggatctc ctgataggta ttcatcagag atttcaggac gtcgacttta tcagctgcga
     8341 cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat
     8401 cgataaggcg cagatccagt tcttctgcat gacccgccag gaagcgcacc agcagtttgt
     8461 tggcattacc caggccaacg tggatttccc cgccggagtg accccctttc agacctttta
     8521 aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg
     8581 aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct tcggagtcgg
     8641 tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac
     8701 cggcttcttc ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag
     8761 ccagaactgc cagtgcagag gccataccaa tgccgttatc cgcacccagc gtggtgccgc
     8821 gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg aagtcatgca
     8881 cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg accggtttac
     8941 gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc tgatcgcgtt
     9001 cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat
     9061 gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag
     9121 ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg
     9181 caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtttg
     9241 agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt
     9301 gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg
     9361 aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc
     9421 gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct
     9481 gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg tgcgttactg
     9541 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     9601 aaccagcgcg agcttaaagt gctgaaacgt gcagaaggcg atggcgaagg cttcatcgtt
     9661 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     9721 gcgcactttg tcaccatctt cgcaaaaccg gccggtcgcc cgctggttga tgattatgtt
     9781 gttgatatcc cgcaagatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc
     9841 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     9901 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     9961 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
    10021 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg atggtaaaac
    10081 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gtattgaccc
    10141 acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac gtaccgacga
    10201 tgatttatac gatactgtga ttggctaccg tggtggcaac tggatttatg agtgggccac
    10261 ccaggcgatg gtgtggcaac aaaaagcctg tgcggaagaa gatccgcaac tcagtggtcg
    10321 acactggctg catgcggcta cgttgtacaa cattgccgcc tatcctcatc tgaaaggaga
    10381 tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg ccgcccagcg
    10441 tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg
    10501 ctttttgcat atgccgaaag gcgatggccc gttcccgaca gtattaatgt gtggtggtct
    10561 ggatgcgatg cagacggact attacagcct gtatgaacgt tattttgcgc cgcgcggcat
    10621 tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca
    10681 ggactccagc ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac cgtgggtgga
    10741 tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tgcgtctggc
    10801 ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct
    10861 gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag
    10921 tcgtttgggg atgcatgatg cttccgatga cgcgttgcgc gtggagctga atcgctattc
    10981 attaaaagtg caaggattgc tgggacgtcg ctgcccaacg ccaatgttat caggctactg
    11041 gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacctcat catctgctga
    11101 cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggtcttca
    11161 ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat tttgccaatt
    11221 tggtaaaaca gttgcatcac aacaggagat agcaatgacg ttaccgagtg gacacccgaa
    11281 gagcagattg atcaaaaaat ttaccgcact aggcccgtat attcgtgaag gtaagtgcga
    11341 agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga
    11401 agtgcgtgaa ttctggggct ggtggatgga gctggaagcg caggaatccc gttttaccta
    11461 cagctaccag tttggtctgt tcgataaagc aggcgactgg aagagcgttc cggtaaaaga
    11521 cactgaagtg gttgaacgac tggagcacac cctgcgtgag tttcacgaga agctgcgtga
    11581 actgctgacg acgctgaatc tgaagctgga accggcggat gattttcgtg acgagccggt
    11641 gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca tccggcaata atcattaaaa
    11701 ctgataggtc atgccaaccg cgacaatatc atcattatta atattcaatt tgttatcgct
    11761 atccagttgg ttgattttat aatcaacaaa cgctgacata tttttgttga aataatatgt
    11821 agccccgaca tcgatataat tgaccagatc ttcatcaccg ataccttcaa tatctttccc
    11881 tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac
    11941 cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt attggcgtca ttttgcgtgt
    12001 ttcagaataa aaagttgcca gataaatatt attggcatcg tatttcagac ctgttgccca
    12061 tgcttctgca cgcttgccag tgccacggct ttgcaggttc tgctcgttgg tgcgatctga
    12121 gttggtatag gcaccactaa tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt
    12181 gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg
    12241 cagggttaag ttcaggccat cgataacgcc gaagaagtcg gtgttccgat acgtcgccag
    12301 accgctggcg cgtttggtca taaagttgtc ggtctgcgcc gaggagtcgc caccaaattc
    12361 cgggaacata tcggtccagg cttccacgtc atacaacgcg cccaggttac ggccatagtc
    12421 gaaagaaccc aaatctttat acttcaatcc ggcaaaagcg agacgcgttt tttgctgtgc
    12481 agtatcactc tccgctttat ttccggcaaa ctccgcttcc caacggccat agccagtcag
    12541 ttgatcgtta atttgtgttt cgcctttaaa accaaaacgg atataactct ggtcgccatc
    12601 tttactgtcg ttatcactca tataatgcat ggctttaact ttgccataga catccagttt
    12661 attaccgtct ttgttatata tttctgcggc ctgtacggat gcagatgcca caatgcccat
    12721 caccactaat gccagagtgc tctttttcat tttcattcct gattttaatt aacgcgcgaa
    12781 tattcagcgg gagagccccg ttgaaaacag gaaagttttt aacctgagat tgttaaagat
    12841 atattacaga ttaataatat tcttaaaatg tggtaattta ttaaatctgt aataaaagcg
    12901 taaacaactg ccgctaggct tgctgatccc gcgcaacaaa acgccatgct ttgcttgcag
    12961 atggttggca accgacgaca gtcctgctaa aacgttcgtt tgatatcatt tttcctaaaa
    13021 ttgaatggca gagaatcatg agtgacagcc agacgctggt ggtaaaactc ggcaccagtg
    13081 tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat cgttgaactt gttcgccagt
    13141 gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt gacgtcgggc gcgatcgccg
    13201 ccggacgtga gcacctgggg tacccggaac tgccagcgac tatcgcctcg aaacaactgc
    13261 tggcggcggt agggcagagt cgactgattc aactgtggga acagctgttt tcgatttatg
    13321 gcattcacgt cgggcagatg ctgctgaccc gtgctgatat ggaagaccgt gaacgcttcc
    13381 tgaacgcccg cgacaccctg cgtgcgttgc tcgataacaa tatcgttccg gtaatcaatg
    13441 agaacgatgc tgtcgctacg gcagagatta aggtcggcga taacgataac ctttctgcgc
    13501 tggcggcgat tctggcgggt gccgataaac tgttgctgct gaccgatcaa aaaggtttgt
    13561 ataccgctga tccgcgcagc aatccgcagg cagaactgat taaagatgtt tacggcattg
    13621 atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg cctcggaact ggcggcatga
    13681 gtaccaaatt gcaggccgct gacgtggctt gccgtgcggg tatcgacacc attattgccg
    13741 cgggcagcaa gccgggcgtt attggtgatg tgatggaagg catttccgtc ggtacgctgt
    13801 tccatgccca ggcgactccg cttgaaaacc gtaaacgctg gattttcggt gcgccgccgg
    13861 cgggtgaaat cacggtagat gaaggggcaa ctgccgccat tcttgaacgc ggcagctccc
    13921 tgttgccgaa aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa gtcatccgca
    13981 tttgcaacct cgaaggtcgc gatatcgccc acggcgtcag tcgttacaac agcgatgcat
    14041 tacgccgtat tgccgggcac cactcgcaag aaattgatgc aatactggga tatgaatacg
    14101 gcccggttgc cgttcaccgt gatgacatga ttacccgtta aggagcaggc tgatgctgga
    14161 acaaatgggc attgccgcga agcaagcctc gtataaatta gcgcaactct ccagccgcga
    14221 aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg aaatcatcct
    14281 caacgctaac gcccaggatg ttgctgacgc gcgtgccaat ggccttggcg aagcgatgct
    14341 tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt gccgatgatg tgcgccaggt
    14401 gtgtaacctc gccgatccgg tggggcaggt aatcgatggc agcgtactgg acagcggcct
    14461 gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc gtgatttatg aagcgcgccc
    14521 gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg tgatcctgcg
    14581 cggtggcaaa gaaacgtgtc gcactaacgc tgcaacggtg gcggtgattc aggacgccct
    14641 gaaatcctgc ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg accgtgcgct
    14701 ggtcagtgaa atgctgcgta tggataaata catcgacatg ctgatcccgc gtggtggcgc
    14761 tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg gtgatcacag gtggtatagg
    14821 cgtatgccat atttacgttg atgaaagtgt agagatcgct gaagcattaa aagtgatcgt
    14881 caacgcgaaa actcagcgtc cgagcacatg taatacggtt gaaacgttgc tggtgaataa
    14941 aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa atggcggaaa gcggcgtgac
    15001 attacacgca gatgcagctg cactggcgca gttgcaggca ggccctgcga aggtggttgc
    15061 tgttaaagcc gaagagtatg acgatgagtt tctgtcatta gatttgaacg tcaaaatcgt
    15121 cagcgatctt gacgatgcca tcgcccatat tcgtgaacac ggcacacaac actccgatgc
    15181 gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg attcgtccgc
    15241 tgtttacgtt aacgcctcta cgcgttttac cgacggcggc cagtttggtc tgggtgcgga
    15301 agtggcggta agcacacaaa aactccacgc gcgtggccca atggggctgg aagcactgac
    15361 cacttacaag tggatcggca ttggtgatta caccattcgt gcgtaaataa aaccgggtga
    15421 tgcaaaagta gccatttgat tcacaaggcc attgacgcat cgcccggtta gttttaacct
    15481 tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg gccgatatag ctcagttggt
    15541 agagcagcgc attcgtaatg cgaaggtcgt aggttcgact cctattatcg gcaccattaa
    15601 aatcaaagag ttaccccata tttaaataca ccacgtttcc tcctgtgccg catttgtgcc
    15661 attgtaacct tggcaattca tcaaaatact gttctgacat caggcagtgc aggtgcagct
    15721 atttaaacca attgctgccg ccattctttg acgtagtcaa tcagggcgcg gagctttggt
    15781 gcaatattgc gacgctgtgg gaaatacaga tagaagcccg gaaattgtgg aagaaagtca
    15841 tcaagcagcg atacaagctt accgctttca atatatggcc tgaaagtttc ctgagtggca
    15901 attgttattc ctccgccggc aagagccagc ctcaacatca gacgcagatc attagtcgta
    15961 atctgcggtt caatcgcaag gtcgaaagtt ctcccgtttt cttcaaatgg ccagcgataa
    16021 ggcgcaacct ccggggactg acgccagccg atacacttat gggtatttcc cccggaggcg
    16081 agaaagcgct ctccacgccc ggccgcaagg atcaggacga cgggggcagg catgaatcct
    16141 cctcctgatg gagacgtaca gaggcgactt ctgccagcac ggagagtgcc agagtatgcg
    16201 catcccgggc tttggggaat atcccgacgg gtgcccggat ttgcgttgtt tcctccctgg
    16261 accatcccag ctcgtggagc ttttgcagac gtaacgtgtg ggttcgatag ctgcccaatg
    16321 cgccgagata aaagggtttt gcttctcgcg cggcctgcaa cactggcagc tcccggttga
    16381 gatcatggca cagcaaaatg accgccgtat cggtatcgat ctgagcgctg gctgaggccg
    16441 gaaaaagatc gaagatatgg ctgtcatagc ctgtggctgc tgcaagactc gcagttgcct
    16501 gcgtctcaag agaacgtccg taaatcatca gcctgacgca tggcctgaac cccacctcaa
    16561 agccattgag attccagccc gttcgggttt gcgtgggcag gcacaccagc gattgtgctt
    16621 gcggatcgta gcgcagcccc gccggttttc tctgttccag acggttcagc acggcgagca
    16681 gaggctgtgc cgagcgtagt ttatggagcg tcagcgtgat cccaccgccg cagggcagaa
    16741 cgatgtcaaa ccacggcgaa ccttcgccat agcgaatttc gcgatcgcgg cctgagccca
    16801 tcatctccag cgcttcaaaa gcggcagcgg cctccacgca gccgccagag acaaaaccgc
    16861 agtaacgacc atcttcgcga accaccatct gcgccccgag cgggcgcgct gcgccgccgc
    16921 gtatctccac cagcgtcacc agcaccgctc ctttaccgga cattagcgcc tctacggcga
    16981 agcgcagaat ggtcaggtgg tcatcggtga gaaacgcctg ctctggctta tgccagtgtt
    17041 cgtctttatc aaaaagcggg tatgacattg tttctcctta aaccacatcc ggcagcttat
    17101 cgagcagctt atccagagtg atgggataat cccgtacccg aataccggtg gcgttataca
    17161 ccgcgttggc gatagccgcg ctcacgccgc acaggcccag ctcaccgaca cctttggcct
    17221 tcatcgggga ggatatgggg tcggtatcat ccaggaaaat cacctcctgt tttgggatat
    17281 ccgcatggac cggcacctca taccccgcca tatcgtgatt aacgaagtag cccaaacggt
    17341 catccaccgc cagctcctcc atcagtgccg cgcccatgcc catagtcatt gcgccaatga
    17401 cctggctgcg cgcagttttc ggattcagga tgcgtcctgc agcacacaca gcgagcatac
    17461 gccggacccg aacttctccc gtcgcgctat gcacgccgac ctccacaaaa tgcccggcaa
    17521 aggtcgactg ctggtactct ttgctcagtg ttccgaattc aatgctctct tccgctgtca
    17581 gtctgccgcc tgcggtggct tcatgtagcg tggcgcttcg ggtaccgttg gtaatcttgc
    17641 cgtcggcaaa ctgcgactgc tcaggatcaa acccgactgc cgaggcaatc atttcgcgaa
    17701 gcttcataca ggcggcgtaa acgccggagg tggaggtatt cgcgccccat tgtcctccag
    17761 aacccgcaga aaccgggaaa ctggaatcgc cgaggtgaac cgcaacctgc tccagcggta
    17821 cgccaagcat ttccgctgcc gtctgggcca gaatggtgta gctgccggtg ccaatgtcgg
    17881 tcatgtccgt ttctacggta acggtgccgt tttgttcgag gtgaacccga gcacccgatt
    17941 tttccagcag attattgcga aagcccgccg caacaccgtg gccgactagc cactccccgt
    18001 cgcgcacctg tccgggtgtg gcgttgcgct gcttccagcc aaatttatcc gctccggtgc
    18061 gcaagcactc gataagctga cggcgagaga agcagcgcgt cgggtcggcg gggtcaacct
    18121 gagtgtcatt caggatgcga aactcgacgg gatcgatgcc cgctttttcc gccagttcgt
    18181 cgatcgcgat ttcgagcgcc atcagaccgg gggcttcgcc gggcgcacgc atggcgttcc
    18241 cttccggcaa atcaagcgtg gcgagccgca ggccggtatg acgattcgcc ccggcgtaga
    18301 gtaattcgct ttgctgtacc gccgtttccg gcgtgccgcc gggcaggttt ccagaccagc
    18361 tttcatgtga gatagcggtg attttcccgc tctggtcggc accgatacgc aagtgctgaa
    18421 gggtggcggg gcggtgcgtg gtgttattgg gaatagaggg gcgggggagc atcactttaa
    18481 ccggacgttt caccgctcgg gcggcgaggg ccgccagcag cgcatcgctt ctcaggaaca
    18541 gcttgccgcc aaaccctccg ccgatatacg gggagataat acgcacattc tccacgggaa
    18601 ctttcagcgt ttttgccaga tcggtgcggc accagtcaat catctgattt gaggtccaga
    18661 gggtaagctt atttccatcc cagacggcca tcgaggcatg cggctccatc gccatatggc
    18721 tctggtccgg ggtcgtgtag gtagcatcaa tcttcaccgc agcggaggtg aaagccccgt
    18781 caaagtcacc gacgtttttg tcgggcgtgt cttccggcgg ctgattgacg gcctgttttt
    18841 cgtccgccag ggagtaagct cctttattac ggcgatagtg tgcctgcacc agcgaggccg
    18901 ccgctcgcgc ctgttcgaag gtctcggcca ctaccagcgc aatggcctga tgatagtgct
    18961 caatagtggg gccgcctaac agtctggcgg tgtttttgtc gcctttgccg agtgccccgg
    19021 cgttactggc ggtaatgaca gccagtacgc ccggcgcttt ttgcgcggcg tccgtatcaa
    19081 gggcggtgag gcgtcctttg gcaatggcgg aaccgacgat atagccatag gcggcgttgg
    19141 gggcttcttc atgccattcg taggcgtagc gtgccgtgcc ggtagttttc agcggtccgt
    19201 cgatgcggtc atggggacga ccgacaacct tcagctgatc gatcgggttt tcccctgcgg
    19261 gtttatcaaa tttcatgcct gtgccctcgc ttcagccagt acggaggcaa gcgttcgctt
    19321 cgccaacagg agtttaaagg tattttcagc ggtgggatgg gcgctggcga acagcgcgtc
    19381 atatacggcc tgcgccccct gggatagctg agcatccgca gcctcaatgc gccagggctt
    19441 atgtgctact ccgcccagcg cgacgcgccc gctgccgtca ggctgaataa tcgccgcgac
    19501 cgataccagg gcaaaggcgt aggaggcgcg atcgcgcacc ttacggtaga tatgttttcc
    19561 gccgagcggt ggaggtaacg tcaccgcaac gataagctca ccgggaagca gggcggtttc
    19621 aatgtgcggc gttttccccg gtgggtgata aaaatcagcc agtgtgatac tgcgagtctt
    19681 tccctccggc gtgatggttt ccaccaccgc atccagcaac cgcattgcga ccgccatatc
    19741 gctcggatgg gtggctatgc aggcttcact tacgcctacc accgcgtgct gacggctaaa
    19801 gccttcaagc gccgcgcagc cgctcccggg caggcgctta ttgcagggct gattggtgtc
    19861 gtaaaaatag gggcagcgcg tgcgctggag cagattacct gcggtggttg cctgattacg
    19921 taactgacca gatgcgccag cgagcagggc gcgggagagt accgcgtaat cacgacgcac
    19981 gcgctcgtga gccgccaggt cggtgttccg taccagtgcg ccgatgcgca gcccacccgc
    20041 gtcggtcact tcaatcttat cgagcccgag gccgttcaca tcgataaggt gggtgggcgt
    20101 ttcaatttcc agcttcatca ggtccagcag attggtcccg cccgcgataa attttgcgcc
    20161 gggtacgcgc tgagcgctaa gtgccgcctc ggctggggtg ttcactcgtt cataggtaaa
    20221 cgccttcatg attttatctc ccccgcagca tcttcaatgg cggcaaggat gttagcgtat
    20281 gcaccacagc gacagatgtt gccgctcata cgttcacgga tctcatcggc agttgtttct
    20341 ggagcggaaa ccaaatcgac cgtgacgtga ctgggaatgc cgtcctgaat ctcttttagc
    20401 gccgctactg atgagcaaat ttgcccggag gtgcagtagc cgcactggaa gccatcatgc
    20461 ttgataaagg ccgcctgcat ggggtgaaga ttatctggcg aacccaggcc ttcaatggtg
    20521 gtgatctcgg ccccctgatg catgactgca agcgtcaggc aggcattaag cctgcgacca
    20581 ttgaccagca cggtacaggc tccgcactgt ccgtgatcgc aacctttctt ggtaccgatc
    20641 aaatgcagat tttcacgcaa agcgtccagt agcgtggttc gggtatccac ctcaagctgc
    20701 tcggttttgc cgttcacctt cagtgtcagg ggcattatct ctggcgcggg tgtagctgcc
    20761 ggaacgcttg ccgccagcgt agaatgagga taaaccacgg cggtcgccgc tgttgcggcg
    20821 ctcactttaa tcagatcgcg acgggtcaaa cttaaatcgt gcggctcgtg cttcccaacc
    20881 cgattatctt cggggtattc gccttggttg ctcataccag gcctccggta ttctaaaggg
    20941 gaaaataaga gtgcgttgcg agagtgctga tgctttttta aatgttaagc atagtcggcg
    21001 acagcaggag gattattctt aaaacgcgaa tgcagctatg agcccggcta ataaattcat
    21061 gttcgccggg atgttgatga tgatgggagc tggtttattg ctttagttgt acgatgcaaa
    21121 aaccaataag gaaacctgtg attttcagct ctgcatcacc ctgcaaatct ctgtcacttc
    21181 taatataaaa atagggagaa atgatggagc ttatattcat tggcgattag gaaacc
//
LOCUS       DAZFWP020000082        21233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFWP020000082 DAZFWP020000000
VERSION     DAZFWP020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.082"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            247..747
                     /gene="tssB"
                     /locus_tag="ACHK0Z_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             247..747
                     /gene="tssB"
                     /locus_tag="ACHK0Z_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4502903.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            782..1006
                     /locus_tag="ACHK0Z_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             782..1006
                     /locus_tag="ACHK0Z_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502904.1"
                     /translation="MTAHKNISETYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1057..2532
                     /gene="tssC"
                     /locus_tag="ACHK0Z_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             1057..2532
                     /gene="tssC"
                     /locus_tag="ACHK0Z_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4502905.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPWSPADNPVKSFNYHEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2539..2952
                     /gene="tssE"
                     /locus_tag="ACHK0Z_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             2539..2952
                     /gene="tssE"
                     /locus_tag="ACHK0Z_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4502906.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2956..4806
                     /gene="tssF"
                     /locus_tag="ACHK0Z_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             2956..4806
                     /gene="tssF"
                     /locus_tag="ACHK0Z_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4502907.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4770..5852
                     /gene="tssG"
                     /locus_tag="ACHK0Z_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             4770..5852
                     /gene="tssG"
                     /locus_tag="ACHK0Z_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4502908.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPELSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5877..7157
                     /gene="tagH"
                     /locus_tag="ACHK0Z_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             5877..7157
                     /gene="tagH"
                     /locus_tag="ACHK0Z_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU4502909.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7154..7678
                     /gene="tssJ"
                     /locus_tag="ACHK0Z_RS14620"
                     /old_locus_tag="pgaptmp_002924"
     CDS             7154..7678
                     /gene="tssJ"
                     /locus_tag="ACHK0Z_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4502910.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7681..9012
                     /gene="tssK"
                     /locus_tag="ACHK0Z_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             7681..9012
                     /gene="tssK"
                     /locus_tag="ACHK0Z_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4502911.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRAIMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9017..9778
                     /gene="icmH"
                     /locus_tag="ACHK0Z_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             9017..9778
                     /gene="icmH"
                     /locus_tag="ACHK0Z_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU4502912.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYIPCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9787..12542
                     /gene="tssH"
                     /locus_tag="ACHK0Z_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             9787..12542
                     /gene="tssH"
                     /locus_tag="ACHK0Z_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12539..13282
                     /gene="vasI"
                     /locus_tag="ACHK0Z_RS14640"
                     /old_locus_tag="pgaptmp_002928"
     CDS             12539..13282
                     /gene="vasI"
                     /locus_tag="ACHK0Z_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU4502913.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13287..14699
                     /gene="tssA"
                     /locus_tag="ACHK0Z_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             13287..14699
                     /gene="tssA"
                     /locus_tag="ACHK0Z_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4502914.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14808..18242
                     /gene="tssM"
                     /locus_tag="ACHK0Z_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             14808..18242
                     /gene="tssM"
                     /locus_tag="ACHK0Z_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU4502915.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGSRGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHTLGWLKEKRARQPLNGIILTLDLPDLLTADKRRREYLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRDWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGKDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWMPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDAGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18253..19702
                     /locus_tag="ACHK0Z_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /pseudo
     CDS             18253..19702
                     /locus_tag="ACHK0Z_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19629..20111
                     /locus_tag="ACHK0Z_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             19629..20111
                     /locus_tag="ACHK0Z_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4502916.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20155..21069
                     /locus_tag="ACHK0Z_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             20155..21069
                     /locus_tag="ACHK0Z_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502917.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21079..>21233
                     /gene="ykfM"
                     /locus_tag="ACHK0Z_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             21079..>21233
                     /gene="ykfM"
                     /locus_tag="ACHK0Z_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HGU4502918.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWR"
BASE COUNT         5087 a         5712 c         5794 g         4640 t
ORIGIN      
        1 ttattgccat aatacacctg atgcgaattg cacaggttca cacaatctat ttaacgcttg
       61 cagaataccc ctctttacga tcgataaaaa gtccttttct tatttatttt gaagcgtaaa
      121 attcggggtg atgaaggagt ggtgatttta ttttcaccgc cattagggcg gcattgccga
      181 tatttttttg cggtacaaat atgccgtgtt catggcctgt acgagataac atgacggatc
      241 acttttatga gcaaaaaatt tgaaggcagc gtcgcgcctc gcgagcgtat taatatatcg
      301 tatgtcccaa aaacagatgg ccagacggct gaagtggaac ttccgcttaa catgctcgtt
      361 gtcggcgata ccggaaatac gcaggaaaca tccccactgg atgaacgtca ggcagtttcc
      421 gttaacaagc acaatttcgg tgcagtgatg gctgaagcag ccattggcct caattttact
      481 gtccccgcca cgctgaaggg aagcacaaca gacgatgaac tgaatgtggc gctgaatatc
      541 aaatcactgg atgatttttc accagatagc gtggcccggc aggttccgga agtgaataaa
      601 ttgctggaac tgcgtgaagc actgactgca ctgaaggggc cgatgggtaa ccttccggca
      661 ttccgtaccc agctccaggc attgctggaa aatgaagagt cccgcgagca gttgctcaaa
      721 gaaatcggtc tggtgagcaa taaataacca ccactatttc attaaacagg gttatccagg
      781 catgacggca cacaaaaata tctccgagac ataccacctc tctgttgcag acattctgca
      841 ggtggtttac caggtatgtt tcagcccgtc cgttgaaatc aaccaggatg gtgtggctgc
      901 gctgattaca acactggatc gccgtatcag tgatctattg gatgagatta tccatttttg
      961 tgagtttcag cagagcgcgt cgcactggca gcgcgtactt cactgatggc gaaaagccgg
     1021 tttcagaaac acagctaaaa cagaaggatt ttcagtatgt ctttacagga agaggaactc
     1081 gtttccagtc atgccgggca gcctgagcag gcatcgtcgc tgcttgatca aatcatggca
     1141 cagacccgta ttcagcccgg ttcggaaggt tatgacgttg cccgccaggg tgtgacagcc
     1201 tttattgcca gcatcctgca atccactgca tctgccgaac cggtcaataa actggccgtg
     1261 gacagcatga ttgcagatat tgatgaacgc atcagccgcc agatggatgt catcatccat
     1321 gcgcctgcct ttcagcaggt cgaatcgttc tggcgctcac tgaaaaccat ggtggatcgc
     1381 gttgatttcc gtgaaaacat caaggtcaac gtgcttcatg tcaccaagca ggaacttctg
     1441 gaagactttg agtttgcgcc agaaattatt cagtccggat tttataagca cgtttattcg
     1501 tccggattcg gacagtttgg tggtgaaccc atcgctgccg tgctgggcgc ttatgagttc
     1561 aaaaataccg cacctgacat gaaactgctg caatacgtca gtgccgtggg cgcaatggcg
     1621 cacgcaccgt tcctgtcctc cgtttccccg gagtttatgg ggctgaactc gtggaccgaa
     1681 ctgcctaata tcaaagatct ttatgccatc tttgaaggtc cggcctacac caaatggcgc
     1741 gccctgcgtg actcggaaga ttcgcgctat ttagggctta ctgcaccccg cttcctgctt
     1801 cgccagccct ggtcaccagc agataaccct gttaaaagct ttaactatca cgaagatgtc
     1861 agccagaacc acgaagatta tctgtggggt aatacggcct ggatgctggc atgcaatatc
     1921 gcagacagtt ttgccaaata ccgctggtgt ccaaacatta tcggcccgca aagcggcggc
     1981 gcggtgaaag atctgccggt gcatctgttc gaaacgatgg ggcagattca ggccaaaatc
     2041 ccaaccgagg tgctggtcac cgatcgccgc gaatttgaac tggctgaaga gggttttatc
     2101 accctgacca tgcgtaaaga ctctgataat gcagcctttt tctctgcaaa ctcggtacaa
     2161 aaaccgaagc actttccggg aaaagatgca gaaaccaact ataagctggg tacgcagctt
     2221 ccttatctct tcatcatcaa ccggttagcg cactacatca aggtgttgca gcgtgaacaa
     2281 ctggggtcat ggaaagaaag gagcgactta gagagggagc ttaatacctg gatccgtcag
     2341 tatgttgccg accaggaaaa cccgcctgcg gacgtgcgca gccgtaaacc tctgcgcgca
     2401 gccagagttg aggttatgga tgtggaaggc gaacccggct ggtaccaggt tgcgctaagc
     2461 gtgaggcctc attttaaatt tatgggggca aattttgagc tttccctggt tggccggtta
     2521 gacagggagt aacacaccat gataccggat cgggaaagaa ccacaggttc tctttttgag
     2581 cgtatggaag cctcttcggc cagaaaccgg cagggaggca gtattcattc gctcaggcaa
     2641 tccattcgcc agaacctacg aaacattctt aatacccggt ccggtagttg ccggggtgcg
     2701 cctgagctgg gcattgatga accggaaggg gctgaaaact tccgcgaatc catgagtcgg
     2761 gccatcgagc aatgtataga acgctatgaa ccccggatct cacacgctga agtgcaggca
     2821 gtggtttcat ccgcctccag cccgctggat atgacgtttc atattacagc ctgggtgaca
     2881 tttaacgaaa cgcatgaggt gcttgaattt gatatggcac cgaatggcag ccagcattac
     2941 cgggtggact gacaaatgga atttgaagaa cgctatttcc gggaagaact cgattacctg
     3001 cgccagctta gcaagctgct ggcaacggaa aaaccccatc tggcccgctt cctggccgaa
     3061 aaagatgcgg atccggatat tgaacgcctg ctggaagggg tggcttttct taccggcaat
     3121 ctccgccaga aaattgagga tgaattccca gaactgacgc acgggcttat taagatgcta
     3181 tggcctaatt acctgcgtcc ggttccggca atgaccctta ttgaatatac gccggatatg
     3241 gataagtctt ctgtaccggt gttaatcccc cgtaatgagc agtttacaac caacgccggg
     3301 gaaatcagag ttgatgaagt gctgccctct gatgctaaaa aggaggagcc gcctccctgt
     3361 accttcactc tctgccggga tatctggctg ctgcccgttc gcctggagca gattgaaaac
     3421 cgcagtacga cccgtaatgg tgttatcaac atcacctttt cggtcgcacc gggaacggac
     3481 ttccgcacgc tggatctgaa caaacttcgc ttctggctcg gcaatgacga caactatacc
     3541 cgtgaccagc tttatttatg gttctgcgaa tacttgcagg gtgccgacct gactgtgggt
     3601 gaacagcata ttcgcctgcc tgagtttatg ctaaaagctg tcggttttga gccgcaggat
     3661 gccatgctgc cctggccgaa aaacgtccac agcggctacc ggatccttca ggagtatttc
     3721 tgttaccccg atgcgtttct cttttttgat ctttgtggtt gtccggcttt gcctgacgga
     3781 ttgcaggcgg aattctttac cctgcaactg cgtttttcgc gccctttgcc cgtggacatc
     3841 cggctgcgcc gcgattccct gcgcctgtat tgcgcacctg ccattaattt atttatccac
     3901 catgcagaag ccatcacgct ggacaaccgg cgggcagact atccgctggt tcccagccgc
     3961 cattacccac aacattacga tgtattttcc gttaacagtg tggtgagcca ggtccaggat
     4021 atgttcagga aaaaagatct ggggcgtcct gtttcgacgc aggccgcgcg ccagtggcca
     4081 gcctttgaaa gtttcagcca tcagatggaa tacagccgga agcgggaagt ggtgtactgg
     4141 catcaccgga ccaaaacatc cctgttccat cgcggctttg atcataccct tgcctttata
     4201 catgctgatg gcagttatcc gtcagacgaa tctctgctca gtaatgaagt ggtttcggta
     4261 tcgctgacct gtaccaaccg tgagcttccg tcacaaatac gttccggcga tatcaccggc
     4321 acaaccggta aaaatgcggc tgttgcttca tttcgcaaca ttacccgccc gacgcaacca
     4381 ctctggccgg tcattgatgg cagcctgcac tggtccctac tctccgccat gaacctgaat
     4441 tatctgtcat tactggatac ggacgcgctg aagcaggtca tcgccaactt tgatcgccac
     4501 gcaatccatc atccgcagac ggcacggctg tcacaacaaa agctggatgc cattgagcgt
     4561 ctggagaccc gccccgttga tcgcctgttt acgggtattc ccgtccgggg actggcctcc
     4621 acgctgtatc tgcacccgga gccgtttgtc tgtgaagggg aaatgtatct gctcggtacg
     4681 gtgctttcgc attttctgtc gctgtacgcc agcgttaact cattccacat gctgaccgtt
     4741 gtgaacacag aaagccagga gacatggaaa tggacggaaa gaatcgggca gcatcctctt
     4801 atctgagtcc gggaaatccc ccggcagata aagagcagaa tgatccgctg gcacaggttt
     4861 tccataatgc ctgctcttac aatttttttg cgatggcgga gctgttgcac cgcctggcaa
     4921 agggtgaaaa ggggacgcca gaattatccc tgcgtgacga tccggcacag gaaaccctgc
     4981 gtttcagcgc cgatgccagc cttgccttcc cgtgcagtga tatcagtgca ctgaaacggg
     5041 atacgtcagg ggcattcagg atgaccacca cctttatggg gttacagggg agccagtcgc
     5101 ctctccccgg ttattacctc gatcacctgg cctggaaagc ggtacatgaa caaagtccgg
     5161 taggtgattt tctggatatg ttcagccacc gcctgaccca gtttgtctgg catatctggc
     5221 gtaagtaccg ctatcacatc agttttcgta acggcggtgt ggatgcgttc tcgcagcgca
     5281 tgtactccct tgtcgggctg ggccaccgcc agcttcgcga taagctggcg attaatcaca
     5341 gcaaaatgct ggcgtattcc gggatcctgg caaatccggg acgatccccg gaaataatct
     5401 gtggcctggt gtcccactgc tttgatttgt cagaagtgac gctgcaaaac tggcaacgtc
     5461 gcaaggtgga tattgaaccg gatcagcaaa atagtctggg gagctattct cttaaaaatg
     5521 gcgaaaaact ggcgggaaga tcggtactgg gcaactttgt tctgggtaca cgagtgcccg
     5581 agctcagcgg aaaattccag ctcagtatta ccagcctgac ccgaaaacag ttcctctctt
     5641 ttctgccgtc cggcgaaaac ttcctgccac tgacgatgtt cgtgtccttc atcctgcgcg
     5701 atcagcttgc ctgggactta catctggggc tggcgccgga gcaggtgggc gcaatgcgcc
     5761 tgggcgataa caaaagtgcg ctgctgggct ggaccagctt cctcggcaca ccggaagaac
     5821 gaccctcagt caccatcagg gtacggtcgt aaattatgat aatcacggag cagaatatgc
     5881 cggaagagaa actacagacg ctttcattgc aggtcattaa cggcagtgag ctggaaagcg
     5941 gacgggcggc gcgctgtctg ttcacacagc agggaaatgt cggccatggc cccgaatgcc
     6001 actggtcggt acaggatcgt cagcagagca ttccggccca ggcttttacc gttatcctgc
     6061 acgatggcac attttgtctg cgcccgcaga cggcacaact gtggctgaat caggcaaaag
     6121 tcacagcaac atcagaccta atacagttgc gccagggcga tgagatccag atcggacggc
     6181 tgatggtgag ggttcatctg aaccggggag atattcccca ttacgatgag gaaatggcca
     6241 ctcccgaaac catcgttacc aatcgcgata tgctcacgga taccctgcta tcaacggagg
     6301 gtacgccaca ctatccggga atgactcacc ggcaccaact tgcagacacc gtggtaaacg
     6361 gtttttctgc cgatccactc caggcacttc agtccgaaag cctgattacc acgggcgatc
     6421 cgctttcagg cattgcggct gtccggccat cggcaccgct gtccgatccg gcaagtaatg
     6481 gggggatcaa tactccgttt atggatctgc cgcccattta tgccagcccc ggcgatcgta
     6541 atgacgacgt ctctgcggca gaaatggcgc aacgccacct tgcggtcacc cccttactgc
     6601 gcggtcttgg cggctcgctt accatgagca attccgacga tgcggatgat tttctggagg
     6661 aggccggacg aacgttacag gccgcaataa aaggtctgct cgatttgcag cagcagcgta
     6721 acagcctctc agacaaacat ttgcgcccgc tggaagataa cccgctgcgc ctgaacatgg
     6781 attacgccac cgcgctcgac gtaatgtttg ccgaaggtaa aagcccggta catctggcgg
     6841 ctcccgccgc cgtcagtgaa agcctgcgca atgtccgcca ccacgaagaa gctaaccggg
     6901 cagcgattgt ggagtcgctt cgtgtcctgc tggatgcttt ctcaccacaa aatctgctgc
     6961 gccgctttgt gcagtaccgc cgcagccatg aactgcgcca gccgctggat gatgccggag
     7021 catggcaaat gtacagccat tattacgaag aactggcctc cgatcgccag caggggtttg
     7081 agatgctgtt taacgaggtc tacgcccagg tctatgaccg ggtgcttcgt gaaaaacagc
     7141 gggagccgga agcatgaaca ggaaggcgtt tctggcctgc gtgctgatgt gcgtcatatt
     7201 aaccggctgc gaaacggcga aaaaaatcag tgaggtgatc aaaaacccgg atatccaggt
     7261 cgggagcctg aaggagcaac cttcggaaat aaccgtcacc ctgctgaccg aaccggacac
     7321 caataccaat gcggaaggtg aatcagccgc cgtggatgtg caactggtgt acctgactga
     7381 cgactcaaaa ttgctggcag ccgactatga ccagatagcc agtacgccgc taccggatgt
     7441 gctgggtaaa aactatatcg accaccagga tttcaacctg ttgccggaca ccatcaaaac
     7501 cctgccgccg gtaaagctgg atgagaaaac gcagtttatt ggtgtcgtgg cctatttctc
     7561 cgatgaccag gccactgaat ggaaacaaat cgagacggtg gaaggaaccg gccaccatta
     7621 ccgcctgctg gtgcatgtac gtcaaagcag catcgaaatg aaaaaagagg atgaataatt
     7681 atggcaacca cccgcaacaa ggtgatgtgg caggaaggga tgctgatgcg cccacaccat
     7741 ttccagcaac agcagcgtta caacgactac ctggataacc agcgtttccg ggccatgaat
     7801 gatttatcct ggggatttac cgaactcacc ctcaacaatg aactgctggc gcagggtaag
     7861 atcatgattg acagcgcgtc aggcacactg cccgacggca ccgtcttttc tatccccgac
     7921 caggacgcac tgcccgatcc gctgcacccg caaaattttc cggacgaaag aagccgcaat
     7981 atctacctcg ctctgccggt cgccagtgat gtgagaaatg aaatcagcga cggacggcga
     8041 atcgggcgtt accggctgaa ttatgccgat gtccgggatt tgcattcaga agaaggtgac
     8101 gcgcgaacat tgacgctggg tcaactgacg ccgcgcatta tgagcggtgc agaagatatg
     8161 agcgcctata ttacgctccc gctttgccgt atcagcgatc gccatgccga tggctccctg
     8221 acgttggatg acgattttat cccctcctgc cagaatattc aggtcagtaa gaaactgcgt
     8281 gtttatctca aagaggtaca gggggccatt ggcggacggg caagcgatct ggcaaaccgt
     8341 attggctctc cggcgcagag cggcatcgcg gatgtggcgg aatttatgat gttgcagtta
     8401 cttaaccgta accagacccg gtttacccat cgcgcccgcc gatcccagct ccacccggaa
     8461 gatttctacc ttgatcttgc tgggttgctg ggtgaactga tgacctttac cgagccgtcg
     8521 cgcctgccct gcccgcttga tgtgtatgat catcatgatc tgaccaaaac atttaaaaca
     8581 ctgttaccgg aagtcaaacg ggcgctacat accgtactgt cgccaagagc ggtcaatctg
     8641 ccgctgcatc tgcgtgacgg tatctggcag gccgatatcc atgacacaga actgctgcaa
     8701 tctgccacct ttgtgctggc cgtggcggca aacgtgccgg tcgatcagat ccagcgtcag
     8761 tttatccagc agtcgaaaat ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag
     8821 atccccggta ttccattgcg tgccattatg gtggctcccc gccagcttcc ttatcattcc
     8881 gggttcagct atttcgaact cgacaagagc ggacaggcct ggacagaaat ggctgccgcc
     8941 ggggccgttg cactgcatgt atccggcagt ttcccggatc tgaacatgca actgtgggcg
     9001 ataagagggt aagacgatgg atgaggggtc actgtctctg ccaccattta ccggatacga
     9061 cgaaaaaagc cagcgcaact accatctcgc gcttcgtggc aacagcctga atccgatgat
     9121 cgacgcggcc acgccgctac tcggtatggt aatgcgcctt tcaaccatga acagccagac
     9181 gatgccggag catctttttg ctcaggtggt gacggatgtt caggccgtgg agcaactgct
     9241 tcaggagcag ggctacgaac cgggcgtgat tatctcgttt cgctacatcc cctgcacgtt
     9301 tattgatgaa gcggcgctcg gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc
     9361 attgctggtc catttccaca acgaagcctg gggtggagag aaagttttca tcctgctgga
     9421 gcgcctgatc cgtgaaccca aacgttacca ggatctgctg gaatttctct ggatctgttt
     9481 ttctctggga tttcgcgggc gctacaaagt ggcagcacag gatcaggggg aatttgagca
     9541 gatctaccgc cgtctgtacc acgttctgca taaactgcgc ggcgatgccc ctttcccgct
     9601 gttacaccag gacaaaaaaa cccagggcgg gcgttatcag ctcatcagtc gcctgactgt
     9661 taaacatatt ttttgtggtg gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt
     9721 gcggctggac agccagacgc aggacatcct gcaccagcta aacaaattac ttcgataagg
     9781 acgtttatga tccagattga tcttcccacg ctggtaaaac ggctgaacct gttctcccgc
     9841 caggcgctgg agatggccgc ctctgaatgt atgagtcagc aggcagcgga aattaccgtc
     9901 agccatgtgc tcattcagat gctcaccatg ccacgcagtg acctgcgggt tattacccgc
     9961 cagggcgata ttggcatgga agagttgcgc caggcgctga cggtagagaa ctacacaacc
    10021 gcccgttctg cggacagcta cccggcgttt tccccgatgc tggttgagtg gctgaaagag
    10081 ggctggctgc tggcgtcggc tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg
    10141 ctggccctgc tgcattcgcc gctgcgttat ataccgcctg ctgccgcccg gctgttgacc
    10201 gtgaccgtct gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc
    10261 cggatgcaga cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc
    10321 gctatgccaa aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc
    10381 gcgaccacga aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg
    10441 tggtggtggg cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca
    10501 tcgtggcagg ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacctgg
    10561 gcgcattgca ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga
    10621 tggcggaggt catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc
    10681 tgattggcgc gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc
    10741 tggcgcgcgg cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact
    10801 tcgaaaaaga tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg
    10861 ctgccgaagc caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg
    10921 tgctgattga tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg
    10981 ggcgtcagtt accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca
    11041 tcaacctgtc gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg
    11101 aggcggaaat tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac
    11161 ggatgaccga ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag
    11221 cggcctggca ccagcagcag acgctggtcc gggagattat tgcgctgcgc cagcagttac
    11281 tgggcgtggc agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc
    11341 agccagagtc agagtcagaa caggataata ccggtgccgt accggctgat gaggccgaca
    11401 gagaacagcc ggaagagacc gctgaaacag tttccccggt acaacgtctg gcacatctca
    11461 ctgccgaact ggacgccctg cataacgacc ggttgctggt ttccccgcac gtcgataaaa
    11521 aacagattgc ggcggtgatt gccgaatgga ccggcgtgcc gcttaaccgc ctgtcgcaga
    11581 atgaaatgtc ggtcatcacc gacctgccaa aatggctcgg cgacaccatc aaaggccagg
    11641 acctggcgat tgccagcctg cacaaacacc tgctgaccgc acgcgccgac ctgcgccgtc
    11701 cgggacgccc gctgggcgcg ttcctgctgg ctggccccag cggcgtgggt aaaactgaaa
    11761 ccgtcctgca actggcagag ctgctctacg gcggtcgcca gtacctgacc accatcaata
    11821 tgtccgagtt ccaggagaaa cataccgtct cgcgactgat tggttcgcct ccgggctacg
    11881 ttggctacgg tgaaggcggc gtactgaccg aagcgattcg ccagaaaccc tactcggtag
    11941 tactgctcga tgaagtggaa aaagcgcacc cggatgtgct caacctgttc taccaggcgt
    12001 tcgataaggg cgaaatggca gacggtgaag gccgcctgat tgactgcaaa aatatcgtct
    12061 tcttcctgac gtccaacctc ggctaccagg taatagtcga gcatgccgat gacccggaaa
    12121 ccatgcagga agtactgtat ccggtgctgg ccgacttctt caaacctgcc ctgctggcgc
    12181 gtatggaagt ggtgccgtac ctgccgctgt cgaaagagac gctcgccacc atcattgccg
    12241 ggaaactggc ccgcctggat aacgtgctgc gcagccgctt tggcgcggag gtgattatag
    12301 aaccggaagt gacggacgaa atcatgagcc gcgtcacccg cgcggaaaac ggcgcgagga
    12361 tgctggaatc ggtcatcgac ggcaatatgc tgccgccgct ctccctgctg ctgttgcaga
    12421 aaatggcggc gaatacggcg gttgcccgga ttcggttgtc ggcagtggac ggcgcattta
    12481 cggcagacgt ggaagatgct cagaacgacg agtccgtcac aaaggatgaa acggttttat
    12541 gagcaggttg cgcgttttcc ggcagacagg atggtttatc gcgggcctga tgacgggctt
    12601 acccgccact gccgcgcccg cagaagcatc gtcaatggcc ggtgtggcgg tcgccgtcgc
    12661 caccacgacc ccgccggatg cgacagccac gttacaggcc atgcagtcct gtcggcggga
    12721 gtccgcggcg ctggagcggc ttgactgcta tgaccgcctg ctggcaccgc tgtccccgtc
    12781 agggtttgac ggggcgctgg tcaaagccgg ttttgtgggc gaagcctgga cccgcgccac
    12841 ggaacaggaa aaacaccgcc agggcaacac cacagagctg ctggtgacgc aggtgccggg
    12901 ggagcgcccg acggtggtga taaccacccc ggctatcggt catgttccgc cacgcccggt
    12961 gctgatgttc agttgcgtgg acaatatcac ccggatgcag gtggcgctga tgcaccctct
    13021 ggatgtccat gatatcgccg tcactctgaa tgccgacaac cgggcattgc gcagccactg
    13081 gtttgtgcgc gagaacggca ccctgctgga gtccagccgg gggttatccg ggattgatga
    13141 aattaagcaa ctgttcggcg caaaaacgct gacggtggat accggcgcag acaatgccgc
    13201 cggaaagctg acctttaaca ttgacgggct ggcaagggcg attgctcccc tgcgcgatgc
    13261 ctgccactgg gcgggagaat aaaaatatgg caatggactt acgcgatccg aatgtctgga
    13321 tatcgcacct gctggaaaac ctgccggaag agaaactggc atcggcgctg aaagatgaca
    13381 acccgaactg ggagtatatc gacggcgaaa tcgtcaagct gggatcgctg gcccacagtc
    13441 aactggatat cccggaactc cagcgccggg ggctggtcat tctggcttcg gagagtaagg
    13501 attttcgtct gctggcgcat ctgctgagaa ccctgcaaca cgccggtgat cctcatctcg
    13561 cattgcgcct gctggcgctg tatgtggagc attactgggc ggtggctgcg ccgcagaata
    13621 tggcgcataa aaaacgcttt gccagccagg tcattaaacg ctttgaaaca ggtattgaag
    13681 ttttttcaca gaacgccgcc acagcgcagc gcgatgcgct gtcaggggaa ctggcaaaac
    13741 tggcgcaatg ctggcagtca cataacgccc cggaactggc acaggctact gatgatcttt
    13801 ttgccctgta ccagcgtgcg tttaatcgtg cggcaccggc tcctgtcccc actccggcgg
    13861 cctccggtag ttcaccacag accaccgcca cgtctgaaag cggcgtgacg caacccagtg
    13921 ctccggctcc ccaaatcgtc atcgacagtc acgacgacaa agcctggcgc gacacgctgt
    13981 taaaagtggc ggccatttta tgtgaacgcc agccggattc accgcagggc taccgtctgc
    14041 gccgccatgc gctttggcag aacatcacca gtacgcccca ggcggaaagc gatggccgta
    14101 ctccactggc tgcggtctcc gccgatatgg tggcagatta ccatgctcag ctcggtagtg
    14161 ccgatatggc gctgtggcag caggtggaga aaagcgtact gctggctcct tactggctgg
    14221 atggtcactg cctttctgca cagacggcgc tgcgtctggg ttacaaacag gtggcagacg
    14281 ccatccgcga tgaggtcatc cgcttccttg agcgtctgcc ccagcttacc gggctgctgt
    14341 ttaatgacca cacaccgttt atcagtgagc agacgaaaca atggctggca gcttcgcccg
    14401 acgccaaagt tgcaccggtt gcgcaaatcg gtgaggaatc gaaggcagcc agagcctgtt
    14461 ttgctgagca gggtctggag gcggcgctgc gatatctgga catgctaccc gaaggcgatc
    14521 cccgcgatca gtttcaccgc cagtaccttg ccgcacagtt gacggaagag gcggggctgg
    14581 tacagcttgc gcagcagcag taccggatgt tgttcaggat gggattgcag atgatggtgg
    14641 cagactggga gccgtccctg cttgagcagc tagaacaaaa attcacggca gaacaataaa
    14701 agagaacagg agtttttgtg ttcagattac caacaccccg actactcagc ggactcaaat
    14761 cagccctgcg accggcgatg ccccggttta aagtctctgc tttctggctg ctgatactgg
    14821 cgtggatttt tctgctggtg tggatctggt ggaaaggccc gacgtggacg ctgtatgaag
    14881 aacagtggct caaaccactg gcgaaccgct ggctggcaac ggcggcgtgg gggattattg
    14941 ccctgatgtg gcttaccgtc cgggtgatga agcgcctgca acagcttgaa aaacagcaaa
    15001 aacaacagcg cgaggaagcc attgatccgc tcagtgtgga actgaacgcc cagcagcgtt
    15061 acctcgaccg ctggctgctg cgcctgcaac gccatctcga caaccgccgt ttcctgtggc
    15121 aattgccgtg gtatatggtc atcggcccgg cgggcagtgg caaaacgaca ctgctgcgcg
    15181 aggggtttcc gtcagacatt atttatgccc cggagggctc gcgcggcgca gaacaacgcc
    15241 tgtaccttac gccccatgtc ggtaaacagg cggtgatctt tgatatcgac ggcacactct
    15301 gcgctcccgc tgatgcggat atcctgcatc gccgtctgtg ggaacatacc ctcggctggc
    15361 tgaaggaaaa gcgcgcgcgc cagccgctga acgggattat tctgacactc gatttacccg
    15421 atctgctgac cgcagacaag cgccgccgtg agtatctgct acagacgctg cgcagccgct
    15481 tgcaggatat acgccagcat cttcactgcc agctaccggt ttacgtggta ctgacccggc
    15541 ttgatttatt gcagggtttt gccgccctgt tccagtccct gaacagacag gatcgtgatg
    15601 cgattctggg tgtcacgttc acccgccgtg cccatgaaaa tgatgactgg cgaacagagt
    15661 tgaatgcttt ctggcagaca tgggtggatc gaatgaatct ggcgttgccg gatctgatgg
    15721 tcgctcagac tcacacccgc gcgtctttat tcagtttttc ccgccagatg cagggaagcc
    15781 gtgaacccct ggtgtcactg cttgaaggtc tgcttgacgg cgaaaatatg aacgtgatgc
    15841 tgcgtggtgt ctatctcacc tcgtcactgc aacgcggcca gatggatgac atctttaccc
    15901 agtccgccgc ccgccagtac cggctgggca ataacccact ggcgtcctgg cccctggtgg
    15961 acaccgcgcc ttatttcacc cgcagcctgt tcccgcaggc attactcgca gagcctaatc
    16021 tggcaacgga gagccgtgac tggctgatgc gttcccgtcg ccgcctgacg gttttctctg
    16081 ccacaggcgg cgtggcggca ctgctgctca tcaccggctg gcatcactat tacaacggta
    16141 actatcagtc cggcatcacc gtgcttaagc aggccaaagc ctttatggac gtgccgcctc
    16201 cgcaggggaa agacgactac ggcaacctgc aactgccgct gctgaacccg gtacgcgatg
    16261 ccacactggc ctatggcgac tggggcgacc gcagccgtct ggccgatatg ggactgtacc
    16321 agggacgacg tatcgggcct tatgtggaac agacctatct gcaactacta gagcagcgtt
    16381 acctgccctc gctgtttaac gggctggtca aagcgatgaa cgccgcgccg ccggagagtg
    16441 aggaaaaact cgccgtgctg cgcgtgatgc gaatgctgga ggacaaaagc ggacgcaata
    16501 atgaggtggt gaagcagtat atggcaaaac gctggagcga aaagttccac ggccagcgtg
    16561 atatccaggc acaactgatg tcccatcttg actacgcgct ggctcatact gactggcatg
    16621 cagagcgtca ggcgggcgac ggtgacgcca tcagccgctg gatgccatat gacaagcccg
    16681 tggtatcagc acagaaagaa ctgagcaaac tgcctgtcta ccagcgggtt taccagagcc
    16741 tgaaaacgcg ggcgctgggc gttctacctg ctgacctcaa tctgcgtgac caggtagggc
    16801 caacctttga ccaggtcttt acatctgccg atgacaacaa actggttgtt ccacagtttc
    16861 ttacccgtta cggcctgcaa agctattttg taaaacagcg cgatgaactg gttgaactga
    16921 cggcaatgga ttcctgggta ctcaacctca cccgcagtgt gaaatacagt gacgccgacc
    16981 gcgcggaaat ccagcgccag ttgaccgagc agtatatcag cgactacacc gctacctggc
    17041 gagccgggat ggacaatctg aatatccgca attttgagtc cattggacaa ctgaccgggg
    17101 cgctggagca ggttatcagc ggcgaccagc ctttgcagcg ggcgctgact gtgttgcgtg
    17161 acaacacaca gccaggcgtc ttttctgaaa aactttctgc caaagaacgg gatgaggctc
    17221 tggcagaacc ggactaccag ttgctcaccc gcctcgggca tgaatttgcc ccggaaaaca
    17281 gtactctggc agtacagaaa gacaaagaaa gcacgatgca ggccgtgtat cagcaactca
    17341 ccgagttgca ccgctatctg ctggcaattc agaacgcgcc tgtaccaggg aaatcggcgc
    17401 tgaaagccgt gcagttgcgg cttgatcaga acagcagcga tccgatattc gccacccgcc
    17461 agatggcaaa gacactgcct gctccgctca accgctgggt tggcagactg gctgaccagg
    17521 cctggcatgt ggtgatggtg gaggccgttc actatatgga agtggactgg cgcgacagcg
    17581 tggtgaaacc gtttaacgag caactggcaa ataactatcc gtttaatccg cgttctgcac
    17641 aggatgcctc actggatgcc ttcgaacgct tctttaaacc ggatggcata ctggatacct
    17701 tctaccagca gaacctgaag ctgtttatcg acaatgacct gagtctggag gatggcgata
    17761 acagcgtcat tattcgcgaa gatattattg cgcaactgaa aaccgcgcag aaaatccgtg
    17821 acatcttctt cagtaaacag aacggtctgg gaacatcctt tgccgtggaa acggtatcgc
    17881 tctccggcaa caaacgccgc agtgtactga accttgacgg ccagttagtc gattacagcc
    17941 aggggcgcaa ctataccgcc catctggtct ggcctaacaa tatgcgcgaa ggcaacgaaa
    18001 gcaaactgac gctcatcggc accagcggca acgcaccgcg cagtatcagc ttcagcgggc
    18061 cgtgggcgca gttccgcctt ttcggggccg gacaactgac cggagttcag gatggcaact
    18121 ttaccgtgcg ctttagcgtg gacgctggcg cgatgaccta ccgtgtgcat accgacactg
    18181 aggataaccc attcagcggt gggttattca gccagtttgg tctgtcagac acactgtact
    18241 gaggaggcag ggatggatag taacggactg acacaaacta tcgttaccgg cagtgacccg
    18301 cgcgggctgc cggaattcag tgccatccgc gaggaaataa acaaagccag ccacccgtca
    18361 cagcctgagc tgaactggaa actggtggag tcgctggcac tggcgatttt taaagccaac
    18421 ggtgtggatt tacacaccgc cacctactat acgcttgccc ggacacggac acagggactg
    18481 gcgggattct gcgaaggtgc ggaactgctg gcggcaatgg taagccacga ctgggataag
    18541 ttctggccgc agggcggccc ggcgcgtact gaaatgctgg actggtttaa ctcccgaacc
    18601 ggcaatattc tgcgtcagca aatctccttt gcggaatccg acctaccact gatctaccgc
    18661 acagagcggg cattgcagct tatctgcgat aagctccagc aggtggaact gaagcgcgtt
    18721 ccgcgcgtgg agaatctgct ctattttatg cagaacacgc gtaaacggct tgaaccccag
    18781 ctgaagagta acactgagaa cgccgcacag accacggtca gaacgctgat ttatgccccg
    18841 gaaacacagg catcttccac accagaagcg gtagtgcctc ccctgcccgg cctgcctgag
    18901 atgaaagtgg aagtgcgcag tctgacagag aatcccccac aggccagtgt gataaagcaa
    18961 ggcagtacgg taagagggtt tatcgcaggg atcgcctgtt cagtggctgt cgcctcagca
    19021 ttgtggtggt ggcaggtcta tccggtgcag cagcaactgt tacaggttaa cgacaccgct
    19081 cagggcgcag caacggtgtg gatggcctca cctgaactcg aaaactatga gcgcaggctg
    19141 caacaacttc ttgatacctc cccggtacag ccgctggaaa ccgggatgca gatgatgcgt
    19201 gttgccgaca gtcgctggcc ggaaagcctg caacagcaac aggcctcgac acaatggaat
    19261 gaggcactca aaacccgcgc acagagtagc ccgcagttgc gtggctggtt gcagacccgc
    19321 caggacttac atgcttttgc agatctagtg atgcagcgcg agaaagaggg actaaccctt
    19381 tcctatatca aaaatgtcat ctggcaggcg gagcggggac tggggcagga aacacccgtt
    19441 gagtctctgt tgacgcagta ccaggatgcc cgtgcgcaga agcagaatac agatgcgctg
    19501 gaaaaacaaa ttaatgagcg actcgaaggc gtgttaagcc gctggctgct gctgaagaat
    19561 aacgtcatgc cagaggcggc aaccggtacc acggccgaaa aataattatt taattaaagg
    19621 agattatcat ggcaaatata agttatttat ctttgagtgg agagacacaa gggctgattt
    19681 cagcaggttg ctcaacgctt gattctgtcg gaaacaaagc gcagccagaa cataaagatc
    19741 agatcatggt ttatgcgtta atgcattcca tctcaagaag tcaaaatgtc aatcatcatg
    19801 aattaattat caccaaaccg gttgataaat cctcaccatt gctggcaaaa gcacttagtg
    19861 acaatgaaaa aatggcaata tgtgaattta tactctaccg aaccagtaaa gcgggtattt
    19921 accagcctta ttataaaata aatctgtcaa aagcccgaat atcaagtatt gattttgtca
    19981 caccgcatgc tgtcctggaa aaagaactgg aaccacagga gagaattgcc tttatttatg
    20041 aagatatttc ctgggagcat acgttagccg gtacgaatgc aatgagtaaa tggcaggatc
    20101 gtgtacagta acttactgta ataacataat aaattactat tgcggagaga taaaatggct
    20161 ggtttaagga ataacaacaa cacacagaat gcacagtggg ctgattatgt gggtgacatt
    20221 cttcgggggg ctcagccgat aaaccagcta gtacctcagc acccttatct taacgacgtc
    20281 ccactcattg atgagttaag gcatcagaac actcatcatg ttattctctt gacacttgat
    20341 gttgcaaaaa aaatactcag cccaataaca tcatttgatt acattcattt tatcactaca
    20401 catccttcag gtataaaaga tacactcgcc tggttagtta acgcgggtaa attaatgact
    20461 gagtttgatg acaatgggaa aattatattt aaccttaatg ccttaaagta tacaaaagca
    20521 agttactttg aaatacttgg agagaaatat ataaaaatca caacatccag cccctggctt
    20581 ttagaaaagc tcggtaaata tattttctct tcacgagcgc ctcaagttct ggaactcgct
    20641 attggctggc gaggagcttt atctgaatca ataaaaggag ttaagttctg tatctggttt
    20701 tcagtagcct ggagaaccat agaatttatt atgagttcag agcgagatct ggttaatttt
    20761 cttggcgatt tttcaatgga tgtggccaaa gcagttatag ccggtggtgt tgcaaccgct
    20821 attggaagcc tggcttcttt tgcctgtgtt agctttggct ttccagtaat tcttgtcgga
    20881 ggagcaattt tactgacagg gatagtgtgt acagttgttt taaatgaaat cgatgctcaa
    20941 tgccatttat cagaaaaatt aaaatatgca attagagatg gactaaaacg gcaacaggaa
    21001 cttgataaat ggaaaaggga aaacatgact ccatttatgt atgttcttaa cactccaccc
    21061 gtgatatgag gattaaccat gcgtttacat gttaaattaa aagagtttct ttcgatgttt
    21121 tttatggcca tattattttt ccctgcattc aatgcttctt tgttttttac cggagttaaa
    21181 cctctatatt caataataaa atgctccacg gaaatatttt atgattggag aat
//
LOCUS       DAZFWP020000083        20765 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFWP020000083 DAZFWP020000000
VERSION     DAZFWP020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.083"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(56..1705)
                     /gene="actP"
                     /locus_tag="ACHK0Z_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             complement(56..1705)
                     /gene="actP"
                     /locus_tag="ACHK0Z_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU4502919.1"
                     /translation="MKRVLTALTATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1702..2016)
                     /gene="yjcH"
                     /locus_tag="ACHK0Z_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             complement(1702..2016)
                     /gene="yjcH"
                     /locus_tag="ACHK0Z_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU4502920.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2216..4174)
                     /gene="acs"
                     /locus_tag="ACHK0Z_RS14685"
                     /old_locus_tag="pgaptmp_002937"
     CDS             complement(2216..4174)
                     /gene="acs"
                     /locus_tag="ACHK0Z_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU4502921.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4566..6002
                     /gene="nrfA"
                     /locus_tag="ACHK0Z_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             4566..6002
                     /gene="nrfA"
                     /locus_tag="ACHK0Z_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU4502922.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIEIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6047..6613
                     /gene="nrfB"
                     /locus_tag="ACHK0Z_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             6047..6613
                     /gene="nrfB"
                     /locus_tag="ACHK0Z_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU4502923.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6610..7281
                     /gene="nrfC"
                     /locus_tag="ACHK0Z_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             6610..7281
                     /gene="nrfC"
                     /locus_tag="ACHK0Z_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU4502924.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLMIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7278..8234
                     /gene="nrfD"
                     /locus_tag="ACHK0Z_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             7278..8234
                     /gene="nrfD"
                     /locus_tag="ACHK0Z_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU4502925.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8254..9972
                     /gene="nrfE"
                     /locus_tag="ACHK0Z_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             8254..9972
                     /gene="nrfE"
                     /locus_tag="ACHK0Z_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU4502926.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9965..10348
                     /gene="nrfF"
                     /locus_tag="ACHK0Z_RS14715"
                     /old_locus_tag="pgaptmp_002943"
     CDS             9965..10348
                     /gene="nrfF"
                     /locus_tag="ACHK0Z_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU4502927.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10345..10941
                     /gene="nrfG"
                     /locus_tag="ACHK0Z_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             10345..10941
                     /gene="nrfG"
                     /locus_tag="ACHK0Z_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU4502928.1"
                     /translation="MKQSQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAQYQRQRD
                     PLHQFASQQTPEAQLQVLQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11283..12596
                     /gene="gltP"
                     /locus_tag="ACHK0Z_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             11283..12596
                     /gene="gltP"
                     /locus_tag="ACHK0Z_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU4502929.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTIAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12674..13363)
                     /gene="yjcO"
                     /locus_tag="ACHK0Z_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(12674..13363)
                     /gene="yjcO"
                     /locus_tag="ACHK0Z_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU4502930.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13457..15604)
                     /gene="fdhF"
                     /locus_tag="ACHK0Z_RS14735"
                     /old_locus_tag="pgaptmp_002947"
     CDS             complement(13457..15604)
                     /gene="fdhF"
                     /locus_tag="ACHK0Z_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15185..15187),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGU4502931.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15802..17268)
                     /gene="mdtP"
                     /locus_tag="ACHK0Z_RS14740"
                     /old_locus_tag="pgaptmp_002948"
     CDS             complement(15802..17268)
                     /gene="mdtP"
                     /locus_tag="ACHK0Z_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU4502932.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17265..19316)
                     /gene="mdtO"
                     /locus_tag="ACHK0Z_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     CDS             complement(17265..19316)
                     /gene="mdtO"
                     /locus_tag="ACHK0Z_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU4502933.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEVRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19316..20347)
                     /gene="mdtN"
                     /locus_tag="ACHK0Z_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             complement(19316..20347)
                     /gene="mdtN"
                     /locus_tag="ACHK0Z_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU4502934.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20366..20641)
                     /gene="ytcA"
                     /locus_tag="ACHK0Z_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             complement(20366..20641)
                     /gene="ytcA"
                     /locus_tag="ACHK0Z_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU4502935.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4673 a         5509 c         5691 g         4892 t
ORIGIN      
        1 tgcatgttgg agaggaagag tattccccgg tcaggcgacc ggggaagggg agagattaat
       61 gtgcgcggcc ttgctcaacg ccaaagccgg tctgggagcg gataaactgt gcacggaaca
      121 gttcacgctc gcgcgcacct tccgctgagt tatcggttgc cgagaagaac cagatgccga
      181 ggaatgccac gctgatagag aacagcgccg ggtattcata cgggaagatg gctttttcgt
      241 gaccaaggat ctgtacccaa atcgtcgggc cgaggatcat cagtaccact gcggtaatca
      301 gccccagcca gccacccatc atcgcgccac gagtggtcag tttcgaccag tacatagaaa
      361 gcagaatgat cgggaagtta cagctcgctg cgatggcaaa cgccaggcct accatgaagg
      421 cgatgttctg attctcaaac agcacgccga ggataatggc aatcacacct aaaatcagca
      481 cggtgatttt cgatacccgc agttcttcac gttcggtcgc gccttttttg aagacgttag
      541 cgtacaggtc atgcgaaacc gctgatgcgc ccgccagcgt cagaccagca accaccgcaa
      601 gaatggtggc aaaagcaacc gctgaaataa aaccgaggaa caggttgccg cccactgcat
      661 tcgccaggtg aacggccgcc atgttgttac caccaatcag atggcccgcc gcgtctttat
      721 attccggatt cgcaccaacc agcatgatcg cgccgaagcc gataataaag gtcagaatat
      781 agaagtagcc cataaacccg gtggcgtaga acacgctctt acgtgcttcg cgggcatcgc
      841 tgactgtaaa gaagcgcatc agaatgtgcg gcaagcccgc cgtaccaaac atcagtccca
      901 gacccagaga gagcgcggag atcggatctt tcaccagccc gcccggcttc atgatgtcga
      961 cacctttcgg gtgtaccgcc atcgcttcac tgaacagatt gttgaagcta aagccgacgt
     1021 gtttcatcac cataaaggcc ataaagctgg caccgaacag caacagcacg gctttgataa
     1081 tctgtaccca agtggtcgcc agcatgccgc cgaacaggac gtacatcatc atcagcacgc
     1141 cgaccagcac caccgcaatg tgatagttaa ggccaaacag cagctcgatc agtttacctg
     1201 cgcccaccat ctgggcgata aggtaaagcg ccaccaccac cagagaacca caggccgaaa
     1261 gaatacgaat cggcccttgt ttcagacggt aagaggccac atcggcaaag gtgtagcgcc
     1321 ccaggttacg cagacgttcg gcaatcagga acaaaatgat cggccagccc accaggaagc
     1381 ccagcgagta aattaagccg tcatagccgg aggtaaacac cagcgcggag atccccaaga
     1441 atgaggcggc ggacatatag tccccggcaa tcgccagccc gttctggaag ccagtgatat
     1501 tgccgcctgc ggtgtagtag tcgctacgag aacgtacgcg ttttgatgcc cagtaggtaa
     1561 tgccgagcgt aaacacgacg aaaatcagga acataataat cgcctgccag ttcgttggct
     1621 ggcgctctac ggccccgcta atagcatccg cggcgttagc tgcgaaaggg agtgtggcgg
     1681 taagcgccgt cagaactctt ttcatgatgc ttgtacctca tgcaggactt cattattaag
     1741 acggtcgaat tcgccgttcg cccgccagat gtagataccg gtgagaacaa aggagatcac
     1801 aatcactcca acaccaattg gaataccgcg tgtgacgctg gtgttcggat tcagcggcgt
     1861 gcccagccag ccgggcgcga aggcgatcag taaaataaag ccgatataaa ctgccagcat
     1921 aataatcgac aggatggtgg caaaccgttg ccgtttttcg actaactccc tgaaatgcgc
     1981 attgtcttct atccgctgat aaatagtgcc attcatcaca gattctccag aggtaatgta
     2041 gggattgttt taattcccgc tcccttatgg gagaaggtta acgctcgggt aacccttgcc
     2101 gaatgtaggc cggataaggc gtttacgccg catccggcaa tcaatgcctg atgcgacgct
     2161 gtcgcgtctt atcaggccta caaaccgtta ccgactcgca tccggcaatt gtgggttacg
     2221 atggcatcgc gatagcctgc ttctcttcaa gcagcttctc gactacgcca ggatcggcaa
     2281 gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc aattttgcgc agaatacggc
     2341 gcataatttt gccggagcgg gttttaggca gggagtcggt ccagtgcagc acgtctggcg
     2401 tcgccagcgg gccaatctct ttacgcaccc agttgcggac ttctgcgtac agttctggtg
     2461 acggttcctc cccgtgatta agcgtgacgt aggcgtagat cgcctgacct ttaatattgt
     2521 gcggaatacc tactacggcg gcttcggcaa tcttcggatg cgccaccagc gccgactcaa
     2581 tctctgccgt ccccagacgg tgaccggaga cgttcagcac gtcgtccaca cgcccggtga
     2641 tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc gctgaaatac atatttttga
     2701 aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc aaacagcgta cgcgcctgac
     2761 ccggccagga gtcggtgatc accaggctgc cttcggtagc gccttccagc gggttacctt
     2821 cgttatcgac cagcgccggt tgcacgccga agaacggacg tgttgccgaa ccggctttca
     2881 gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc agtttcggtc tgccaccagg
     2941 tatcgaccac cggacatttc tcgttgccga tttttttcca gtaccactcc cacgcttccg
     3001 ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga cgaacggtcg gtgccttcga
     3061 tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt gggtgcggta tagagaatat
     3121 tgacctgatg cttgtccacc acctgcgcca tacgggcagg cgtcggccag ttgggtacgc
     3181 cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc gtacagcaag taactgtgtc
     3241 cggtcaccca gcccacatcg gcggtgcacc agtagatatc acccggatga taatcaaaga
     3301 catatttaaa ggtcagcgcc gcgtacacca gataaccgcc ggtagtatgc agcacacctt
     3361 ttggcttacc ggtagaaccg gaggtgtaga gaataaacag cggatcttcg gcgttcatct
     3421 cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc gtgccaccac aggtcgcgcc
     3481 cttcctgcca gtcaattttc ccgccagtac gcttcagtac caccacatgc tctacgctgg
     3541 tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt cagcggaata ctgcgcccgg
     3601 cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt ggaatcaata atgcgcccgg
     3661 caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg caccgcgcca atgcgggcgc
     3721 aggccagcat cgcaaccgcg gcttccggca ccatcggcat ataaatcgcc accacatcac
     3781 cttttttaat gcccagctcg agcagggtat tggcgaagcg gcagacgtcg cggtgcagct
     3841 ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc ttcccagatg atggcggtgc
     3901 gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc cgccagattc agcgtgccgt
     3961 cctcgtacca tttaatggac acattaccgg gggcaaagga ggtatttttc accttctggt
     4021 acggtttgat ccagtcgaga atttttccct gttcgcccca gaaggtatca ggtacgttaa
     4081 tagattgttg atacatcgcc tcgtactgct gagggtttat caggcaacgg tctgcgatgt
     4141 tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt gttctccttg tgggatgtta
     4201 ataatatgtg gcataagcgt taaatgtagg ggtattggca gttttgttta gtatttgggc
     4261 gacagatcac gcaaaagtag aattgtgcaa ataaacggca gggtaatttt tgaaggtcac
     4321 caacaaaagt tgattaattc tttgaggaac atgcagttat gcatgctgtt gaaaaagagg
     4381 aagatactga ctaactctaa agtggtattt tacatgcact tacaattgat taaagacaac
     4441 attcacagtg tggttatttg ttacacatag gggcgagcaa tgtcatgaca gtgtaggtgc
     4501 ggttactgtc gtgaaaacaa taaaaaccgc cattgcaaca atggcgcaat tcggatgaag
     4561 cccctatgac aaggataaaa ataaacgcac gccgtatctt cagcttattg attccttttt
     4621 tctttttcac ttctgttcac gctgaacaaa cggcagctcc cgcaaaacct gtaactgtgg
     4681 aagcgaagaa tgaaaccttt gccccgcagc atcccgatca atatctctcc tggaaagcca
     4741 cctcggaaca gtcagagcgt gttgacgccc tggcggaaga tccacggctg gtgatcctgt
     4801 gggcggggta tcccttctcg cgcgattaca acaagccgcg tggacatgcc tttgctgtga
     4861 ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa cgctgaagat ggtccgctac
     4921 cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg tctgatccag aaagacggcg
     4981 aagatggcta cttccacggt aagtgggcgc gcggcggccc ggaaatcgtc aacaacttag
     5041 gttgtgccga ctgccataac accgcctcac cagagttcgc caaaggcaaa ccggagttaa
     5101 ccctttcccg tccgtatgcg gctcgcgcga tggaagccat tggtaaacct tttgagaaag
     5161 ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg ccatgtggag tattacttcg
     5221 acggcaaaaa caaagcggtt aaattcccgt gggatgacgg catgaaagtc gaaaatatgg
     5281 agcagtatta cgacaaaatt gccttctctg actggactaa ctccctgtcg aaaacgccaa
     5341 tgctgaaagc gcagcacccg gaatatgaaa cctggacagc gggcattcac ggtaaaaaca
     5401 acgtgacctg tatcgactgc catatgccaa aagtgcagaa cgccgaaggc aaactctaca
     5461 ccgaccataa aattggtaat ccgtttgata acttcgccca gacttgtgcg aactgccata
     5521 cccaggacaa agctgccttg caaaaagtgg tcgcggaacg taagcagtcg attaacgacc
     5581 tgaaaatcaa ggttgaagat caactggttc acgctcactt cgaagcgaaa gcagcgctgg
     5641 atgcaggcgc gacggaagcc gaaatgaagc cgattcagga cgatatccgt catgcccagt
     5701 ggcgttggga tctggcgatc gcttcccacg gcattcatat gcacgcaccg gaagaaggtt
     5761 tacggatgct cggtactgcg atggataaag cggcggatgc acgcaccaaa ctggcgcgcc
     5821 tgctggcgac caaaggcatc acccatgaaa tcgagatccc ggatatctcg accaaagaga
     5881 aagcccagca ggccattggc ctgaacatgg aacaaatcaa ggccgagaag caggacttca
     5941 tcaaaacggt gatcccgcag tgggaagaac aggcacgtaa aaatggtctg ttaagccaat
     6001 aaccccgttc cgactcgcaa ggggcggaaa acacaatgga gtgaatatga gcgtattacg
     6061 ttcgttatta actgctgggg tgctggcgtc gggcctgttg tggagcctga acggaattac
     6121 cgctacccct gcggcgcagg catctggtga tcgctacgaa gttacccagc agcgtaaccc
     6181 ggatgccgcc tgtctggact gtcataagcc agatatcgaa ggtatgcatg gcaaacatgc
     6241 ttccgttatc aacccgaata acaaactgcc ggtcacctgc accaactgtc acggccagcc
     6301 atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc tttaacgagc cgatgtacaa
     6361 ggtcggagag cagaacagcg tctgtatgtc ctgtcatctg ccggaacagt tgcaaaaagc
     6421 gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc gccagctgcc attccctgca
     6481 tccacagcaa gatacgatgc agacgttaag tgacaaagga cggattaaga tttgcgtcga
     6541 ttgccacagc gatcagcgca ccaatccgaa ctttaacccg gcgtctgttc cgttgcttaa
     6601 ggagcagcca tgacctggtc tcgtcgccag tttctcaccg gcgtcggcgt gctggcagcc
     6661 gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga atatcaatgg ggtgcgttac
     6721 ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg cctgtatgga tgcttgtcgg
     6781 gaagtgaaca aggtgccgga aggcgtctcg cgcctgatga ttattcgtag cgagccgcag
     6841 ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt cttgccagca ctgcgatcat
     6901 gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc gtgatgccgc tagcgggatt
     6961 gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact gcatcgccgc ctgtccgtac
     7021 cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca aatgcgattt ctgccgtaag
     7081 accaatttgc aggccggtaa gctgcccgcg tgcgttgagg cttgcccgac gaaggcgctg
     7141 acgtttggca atctggacga tcctaacagt gagatttcgc aactgctgcg ccagaagcct
     7201 acttaccgct acaagctggc gctgggaacc aaaccgaagc tgtaccgcgt accgtttaaa
     7261 tacggggagg tgagccaatg acgcagactt ccgcatttca ttttgaatcg ctggtgtggg
     7321 actggccgat tgccatctac ctgtttttga ttggtatttc tgccggtctg gtgacgctgg
     7381 ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc agacagcacg ttgctgcgca
     7441 ccacgctgat tgtcggacct ggcgcggtga tcctcggtct gttgatcctc gtcttccacc
     7501 tgacaagacc gtggaccttc tggaagctga tgttccacta cagttttacc tcggtgatgt
     7561 cgatgggggt gatgctgttt cagctctaca tggtggtgct ggtgctgtgg ctggcgaaaa
     7621 tctttgaaca tgatttgctt gccctgcaac aacgctggtt gccgaagctg gggatcgtgc
     7681 aaaaggttct gagcctgctg acgcccgttc atcgcggact ggaaacgctg atgctggtgt
     7741 tggcggtgct gctgggggct tataccggct ttctgctgtc ggcgctgaaa tcgtatccgt
     7801 tcctcaataa cccgatcctg ccggtgctgt tcctcttctc cggcatctcg tccggtgtgg
     7861 cggtagcgct gatcgccatg gcgatacgcc aacgcaaaaa tccgcattcc acggaagcgc
     7921 actttgttca ccgtatggaa atccccgtgg tatggggtga aatcttcctg ctggtggcgt
     7981 tttttgtcgg tctggcgctg ggcgatgacg gtaaagtgcg tgcgctggtg gcggcattag
     8041 gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc cgggctgggg ctgattgtgc
     8101 caatgttgct caaaccgtgg gtcaatcgca gttccggcat tcctgccgtg ttggcggcgt
     8161 gtggggccag tctggtcggc gtgttgatgc tgcgcttttt cattctctac gccggacagt
     8221 tgacggtggc gtaagccaga aaagaggtgg tttctggacg tattccttcc tgaagtcggt
     8281 tttctggcgt tgttgttaag tctcggggtc aacgtgttga ccccgttgac ggcctttgcg
     8341 ggagtgcggt tgcgctggcc tgccatgatg cgactcactt gcatcggcat tctggcgcag
     8401 ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt gttttctcat cagcgatttc
     8461 tcggtcattt atgtcgcgca acatagctat agcctgctgt cgtgggaact caagctggca
     8521 gcggtgtggg gcggccatga aggttcgctg ctgctttggg tgctgctgct ttccgcctgg
     8581 agcgcgctgt ttgcctggca ttatcggcag caaaccgatc cgctatttcc gctgacgtta
     8641 gccgttttat ctctcatgct cgccgcactg ctactgtttg tggtgctgtg gtccgatccc
     8701 ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc tcaatccgat gctgcaacat
     8761 cccggtctta tctttcatcc accgctgctt taccttggct atggcggttt gatggtagcg
     8821 gcgagcgtgg cgctggcgag tttactgcgc ggcgagtttg atggtgcctg cgccagaatt
     8881 tgttggcgct gggcactacc tggctggagc gcattaacgg cggggatcat cctcggttcc
     8941 tggtgggcct attgcgaact gggctggggc ggctggtggt tctgggatcc ggtggaaaac
     9001 gcctctttat taccctggct ttctgccact gcgctgctgc acagtttgtc cctgacacgc
     9061 cagcgggaga ttttccgcca ctggtcgctg ttgctggcga tagtaactct gatgctgtcg
     9121 ctactgggta ccttaattgt ccgttctggc attctggttt cggttcatgc gttcgcactg
     9181 gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac tgattagcct tgcgtctctg
     9241 gctctgtatg gctggcgagc gcgggacggt ggcccggtgg tgcgtttttc ggggttatcg
     9301 cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg cagtgctact gatcgtgctg
     9361 gtgggaacgc tttatccgat gatttacggt ctgctgggct ggggacgcct ctccgttggc
     9421 gcgccgtatt ttaaccgcgc gacgttaccg tttggcctgt tgatgctggt ggtgattgtg
     9481 ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg cgctggtagc tcatgcgggc
     9541 gtgctgttat ttgccgctgg gatcgtggtt tccagcgtca gccgtcagga gatcagcctg
     9601 aatttacagc cgggtcagca ggtgacgctg gcaggataca ccttccgttt tgagcgcctc
     9661 gatctgcaag ccaaaggcaa ttacaccagc gaaaaagcga tagtggcact gtttgaccat
     9721 cagcaacgca ttggtgaact gatgccggag cggcgttttt acgaagcacg tcgtcagcaa
     9781 atgatggaac cgtcaattcg ctggaacggc atccatgact ggtatgcggt catgggtgaa
     9841 aaaaccggag cggatcgtta cgcttttcgc ttgtatgtac aaagcggtgt gcgctggatc
     9901 tgggggggag gattgttgat gattgcgggc gcattattaa gcggatggcg ggggaggaag
     9961 cgcgatgaat aaaggacttc tcacgctact gctgttattt acctgttttg cgcgcgctca
    10021 ggttgtagac acctggcaat tcgccaatcc gcaacaacag caacaggcgt taaatattgc
    10081 cagccagtta cgttgtccgc agtgccagaa tcagaactta ctggaatcca atgcaccggt
    10141 ggcggtcagt atgcgccatc aggtttacag catggtggcg gaggggaaga acgaagtcga
    10201 aatcattggc tggatgaccg aacgctacgg agattttgtt cgctataacc caccgttaac
    10261 gggtcagacg ctggtgttat gggcgctgcc agtggtgttg ttactgctga tggcactgat
    10321 cctctggcga gtgagggcga agcggtgaaa cagtcacaaa taccggtgaa aatgctgaca
    10381 acactcacga ttttgatggt atttctctgt gtcggcagtt atctgttaag cccaaaatgg
    10441 caggcggtac gtgcgcaata tcagcgtcag cgcgatccgc tacatcagtt tgccagccag
    10501 caaaccccgg aagcgcagct tcaggtattg caggataaaa tccgcgctaa tccacaaaac
    10561 agcgaacagt gggcgttact gggcgagtac tatctgtggc aaaacgatta cagtaattcg
    10621 ctgctggcgt atcgtcaggc gttgcaacta cgtggtgaga acgctgaact gtatgcggcg
    10681 ctggcgacgg tgctttatta ccaggccagc cagcatatga ccgcccagac tcgcgcaatg
    10741 atcgacaaag ccctcgcgct ggactctaac gaaatcaccg ctctgatgct gctggcttcc
    10801 gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac tatggcaaaa agtgatggat
    10861 ctcaactcac cccggatcaa ccggacacag ctggtcgagt cgattaatat ggcgaaattg
    10921 ttgcagcgga gatcggatta atcatctggt gatgaagatt atgttattga gctgcataaa
    10981 aaataatcga atgaacatat gccaaaaata atcaccaatc agtattattg cagattaaac
    11041 aaataaaaat ctttccataa caaatggtta ttcattaatc ctgccataaa ctcgcgttat
    11101 tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata ccgcgcaaat
    11161 gatactgatc ataagcgtta aaaaaatcta caaaccaacg caacacaatt catgccctgg
    11221 cagtatgtca cgttctcgcg tttctgaacg gggaacggcg ctccattgag gaagttattc
    11281 tgatgaaaaa tataaaattc agcctggcct ggcagattct gtttgctatg gtgctgggca
    11341 ttctcctggg aagctacctg cactaccata gcgacagccg cgactggctg gtcgtcaatt
    11401 tgctctctcc ggcgggtgat atcttcatcc atctgattaa aatgatagtt gtgccgattg
    11461 tgatctccac gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag ctcgggcgta
    11521 ttggcgcgaa aaccattatc tacttcgagg tgatcaccac catcgctatc attttgggga
    11581 tcactctggc gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag ctggcgaccg
    11641 tcgatatctc gaaatatcag agcactacgg aagcggtaca aagcagttcc cacggcatta
    11701 tgggcacgat tttgtcgctg gtgccgacga acattgtggc gtcgatggcg aaaggcgaaa
    11761 tgctgccgat catcttcttc tcggtgctgt ttggtctggg gctttcctcc ctgcccgcga
    11821 cgcatcgtga accgctggtg accgtgttcc gctccatctc tgaaaccatg tttaaagtga
    11881 ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc gctgattgcg gtgacggtgg
    11941 ctaactttgg tttctcttct ctgtggccac tggcgaaact ggtgctgctg gtgcatttcg
    12001 ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc gcgcctgtgc gggttaagcg
    12061 tctggatcct gattcgtatt ctgaaagatg agctgattct ggcgtactcc actgccagct
    12121 ctgaaagcgt actgccgcga attattgaga agatggaagc ctacggagca ccggcgtcga
    12181 tcaccagctt cgtggtgccg accggttact cttttaacct tgatggttcg acgctgtatc
    12241 aaagtattgc cgctattttc atcgctcagc tgtatggcat tgacctgtcc atctggcagg
    12301 aaatcattct ggtgctgacg ctgatggtga cctcgaaagg gattgctggc gtgccgggcg
    12361 tgtcgtttgt ggtgttgctg gcaacgctgg gtagcgtagg tattccgctg gaaggcctgg
    12421 cgtttattgc tggtgttgac cgtatcctcg acatggcgcg tactgcgctg aacgtggtgg
    12481 gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca caaatttgac cgcaaaaagg
    12541 cgctggctta tgagcgtgaa gtgctgggca aatttgataa aactgcggat caataattga
    12601 agattgccgg ggttatccac ccggcaatgt gtggatgcct gatgcgacgc tggtgcgtct
    12661 tatcaggcct accttaaccg ttggttaatt tttcaaactc ttcacacccg gtatcaaacc
    12721 cttccataca gctcaggttc aaccagtgca gcgccttttg tttgttcttc tcgataaaac
    12781 cttcctcacc gtttaagaac atcattcccg cccagtactc ggaataaccg gttcgggaaa
    12841 ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta atgcccacgc
    12901 cgttggcgta aatcaaacca agcagcattt gggcatcgac ggcagagtcg ttctccagat
    12961 cttccgaggc gttttctaac agcgaaatcg cctttggata atccggttta cccgcttgag
    13021 tatttaccag aatatgcgcc agcgttactt caccttcttt actgcccgct tgcgccgctt
    13081 tctctgcaag aacttttgct tgtggatagt ccagactgac cggattggtg attttgatct
    13141 gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt gcccaatatt
    13201 cggctttgct caaatcgccg gagctaaacc agctgtcggc aagaaaatat tgtgcgcgtc
    13261 ggtccccggc ctctgctgcc tttaaatact ggctgccagg ctcggagtcg ttcgcgtggg
    13321 caaagaatgt caaaaacaac atcaatgcga taattttttt cattttgata atacgattgg
    13381 gtagggagta accagtataa agagttcgaa tggataaaaa aacagcctcc gaaaggaggc
    13441 tgtagaaagg actgtattac gccagtgccg cttcgcgcag gcgagttttc aacttgttgt
    13501 actcgtcaat cacgtactgc tcggcggcgc gctgatcggc gatcggctcg acgcgcacgg
    13561 cgcagtattt gtattccggc gttttcgtaa tcgggcttaa gttttcggta accagctcgt
    13621 tacaggcacc aatccaccac tggtaggtca tgtaaatcgc ccctttgttc ggacgatcgc
    13681 tgacctgcgc acgggtgata attttgcctt tacgcgagtg cacccaaacc aatgcctcat
    13741 cttcaatacc cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca ggttcatcag
    13801 ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg gcaagagtag tgaccaactt
    13861 cacgcaccgt tgacagtacc atcgggtact cgtcggtgag tttgtcgatt ggcgctaccc
    13921 agtcgcaggt gaagaactgc gccagaccgt tcggggtatc aaacttctct ttaaacagat
    13981 aagaagtccc ctgatcggca tctgaagtat cgcggcaagg ccactgaatg aagcccagtt
    14041 cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc aactcatccc
    14101 agatctcctg ggtgttgttg tagtgcatcg gataacccat acgggtggcg atttcactga
    14161 tgatttgcca gtccgttttc agatcccatt tcggttcaac tgctttgaag aaacgctgga
    14221 agccacggtc agccgcagta aacacgcctt catgctcgcc ccacgacgtt gacggtaaaa
    14281 taacatccgc cgccgacgcg gttttggtca taaagatgtc ctgaacgata accagttcca
    14341 gatcttcaaa ggctttacgt actgccgaca gctccgcgtc agtttgcagc ggatcttcgc
    14401 ccataatgta cgcggcacgc acttcgccat gcgctgcgcg gtgcggcagc tcgctgatgc
    14461 gataaccggt atgagcaggc aggctttcca cgccccaggc tttggcgaat ttctcgcggt
    14521 tagccggatc tttcacgtac tggtatcccg gatacgtatc cggcagcgcg cccatatcgc
    14581 aggcaccctg cacgttgttc tgaccacgaa ccgggtttac acccgcatgc ggcttaccga
    14641 ggttaccggt cagcatcgcg aggctggtca gagaacgcac ggtttccacg ccctggtaga
    14701 actgggttac acccatgccc cacaggatgg cggcgctttt cgcctgggca tacatccgtg
    14761 ccgcctgacg aatctcactg gcgctgacgc cggtgatatc ttcaaccgac tccggcgtgt
    14821 agccttcaac gattttacga tactcttcaa agccttctgt acgggaagcg acgaacgctt
    14881 tgtcgtacag attttcttca ataatgacat ggcccatcgc attcaacagc gcgatgttcg
    14941 agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc ggtttcaatt ttgcgcggat
    15001 cgcagacgat aattttcgcc ccgttacgtt tagcgttaat tacgtgattc gccacgattg
    15061 ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa atcggtatta tcaatttcgt
    15121 taatagcatt gctcattgcg ccattaccga ccgattggtg cagacctgca accgatgggc
    15181 cgtgtcagac acgagcgcag cagtcaacgt tattggtacc aataacggcg cgcgcaaatt
    15241 tttgcattac atagttggtt tcgttacccg taccacgcga ggagccggtc gtctggatgg
    15301 catccggacc gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa ttcagtgcct
    15361 catcccagga aacaggttcg agtttgccgc cacgctggcg acggatcatg ggggttttca
    15421 ggcgcggggt caggatctgg gtatcgttaa tgaaatccca gccataataa cccttcagac
    15481 acagggtacc ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt ttgccgttat
    15541 cgacgaccag gttgattttg caacctgatg cgcaataggg gcaaaccgtg acgacttttt
    15601 tcatcggtct cgctccagtt aatcaaatca cgcatacgcg ctctcgacta cagtatgcat
    15661 cttttatgcc acattttatt tggggtcatt ccctgatatt acgggcacta tttattcaaa
    15721 actctgacga aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat accgcgtgac
    15781 agccatcacg cggcagacat tttatttttt ctcgacgacg ggagccgcct gatacccgcc
    15841 gcccagcgat ttcatcaact gaatgctttg gatcacccgg cggctgtcca gcatcagtaa
    15901 tgacatctct tcggcaagta ctggcaaccg ggcttcagtg gcctgtaagc ggctggttaa
    15961 gccgcgctga taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt ccacgcgttc
    16021 agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga cggcaacgtc
    16081 acgcaccgcg ttcagtactg actggttgta acgttcaatc atcatgttgc tggcggcgcg
    16141 cgtgccttcg agattggcat tcaaccgtcc accgtcaaac agcggcaatt tcagacccgg
    16201 gatgaagttg aactggcgac tggttttttt gaataaggta tccaggtgga tggcatcaag
    16261 gccgaaaaac gctttgatat caaagctcgg gtagaacagc gcccgcgcgg aatccacctg
    16321 atctaatgac gcctgaacat accagcgcat ggcttgcaga tccgggcgtc tggcgagcaa
    16381 ctcataagag agtgtcgccg gaatgccggt ctggactcgt ggtaatgcca ccggtttgat
    16441 ctccggcata tcgctggccc ccgcgccaat caatgcacgc agagattctc gcgtttcggt
    16501 gatttgccct ttgacggcag caatttgttt atcgaccgcc agaatctgtg cccgcgcgcc
    16561 gtggaaaggc acttgtgctt ccagaccgtg cgccacttta ctctggtgcg ctttcaccgc
    16621 gtaatcaatc acatcgcgag tttgttctaa cagatcgagc atctgatagc tggcctgcat
    16681 actgtaataa agctgcgcta cgcccgtggt cagcgatagc tctactgctg cggtttctgc
    16741 cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct gagcgatgca caccccacaa
    16801 atcaagatcc agtccggcaa acaaacctac tgtggcttcc gtatagtacg gcccgtccat
    16861 acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg agacgcgttg
    16921 gcggttgagc atccctaacg ctgcgacctg taattgtgaa ccggcatcta acaaatcggc
    16981 ctgggactgc gctttttctt cccgcagttt cgcttcggcg agggtgtgtg aaccacttag
    17041 cgtccgttgg atcagcgcat ccagctgcgg gtcattgagt tgtttccacc actgcgcctg
    17101 cggccagccg gagctggcaa gatgaatatc gtcggccagt ttgatttgtt ccggtttgag
    17161 ctgttgatga ggtgcagaat ccttacgtac cagggcacag ccggaaatca gcgtcgtgct
    17221 gccgagaatg ctgcacaaca gcagacgtga aagttgacga ttgatcattg cgtggctcct
    17281 tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa gcgggcgaaa
    17341 agctggcaga cgtgttgctc ctgttttaat aaggtgggca ggatggcctg cgaggcgggt
    17401 gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc ggcagcgtat
    17461 ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct gtagtgcgtt atccagcgcc
    17521 tgcgccgagt tccaggtggc atcccaggcg tgaagaatat cgcggccctg acgaataacc
    17581 gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg ctccagcgcc
    17641 acgcgttggc acatctcttc acaggcatta aacgccgcat gcagaccgat acgaatttgc
    17701 agataagtgc gcagagccgt gacttcctgc tggcgtggga tccgcattac tttacttaac
    17761 atacccagcg tgccagccag tttttgcggc agtgtgcgcg cttcactttc aggccagaca
    17821 aaggtgtaaa tcaccgcgga caccacggta ccaatgatga tacccatggc gcgatcgcga
    17881 acttccacca ggtcgtacac cgggccaaaa acgttttcga gcgtggcgag cgcgaaggtg
    17941 accaccatct gtgtgccgat ataagaagag cgttcagagc tggtggcaat ccatgcgccc
    18001 aacaggaaaa tcggtgccag cacaaacagc aattcgacaa tattgtccag ccagggcatg
    18061 accagtagcg tgaataacag cgccagaatc gcgccgcaaa aggccccgcc aaaacgcagc
    18121 accatcttct ggtacgacga accgacattt gggttagcga cgatcacgca tgtcagcata
    18181 caggtgtgaa tgccttccca gtccacgccg ctgtagaagg tgtaacagat caaacaggcg
    18241 agcagtgttt ttaccgcgta gcgcatatag tctggattgg taaaagcatc ggcgaccatt
    18301 gatggcggtt tggcggcggg cgttggcggc gtattcgggt ccatctgacc cagttgtaac
    18361 aacgtctggc agatattctc caggttacat tcccgtgccg ccatcgcttc actttcagtg
    18421 atccgccagt cgctttgcca gcactgacct tcagcaacgg catgctgcag cttattgatt
    18481 tctgaggcta atttttgccg aaattcaata attgcctgag aatcagcaaa agaggtggga
    18541 tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct ttgccaccat
    18601 gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa agacattcag tttttgcagc
    18661 gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc cgtcaggtga
    18721 ctaatggcat catcaagccg atcattaagc gcttgatgca tttgcgtaat ggcacgactg
    18781 ggaaaccaca gcacgccgat taacgtcatc agcaaggttg gatagaggcc aacaacgata
    18841 caccacagcg ttaagcgcac gaccacttcc ggatagtcga gcatggcggg gaaggtttgc
    18901 ccgtaaatgg cgacaatggc gacagcgaaa aagaccagtc ccagccgatg ggtgcgcatc
    18961 aaaaacatac agcccatcag gatcggcccg gcgatgatca atcggatcaa cggttcgccg
    19021 tatgaccatt tgtagatcaa aaacaggctg ccgatctcca gcaccgtggc aaccacaaac
    19081 aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa taccgtaaaa cagcactgcc
    19141 agcgataacg ccacaaaagg gatctcaaaa gtcatcgaga tcagaatcac cagcaggcag
    19201 cctacccaga gttgcacgat ctgcggcacg cggccgggtc gccgctcgct taactcttca
    19261 tgaaagaacg ccagcagcct gaccaccggt aatggcaggg agttgagcgc gctcattatt
    19321 gcggctcaag attagcgact gccgaagcgc cgatgcggaa catttccggg tcaggtttat
    19381 cgaccatgat tttgaccgga aaacgctggg caacgcggac ccagttaata gagcgagaaa
    19441 ctttcggcag gccgcccagc accaggccgc cgtcatccgg tagcacgccg tagccaatcg
    19501 aatccacttt accctcgaag gttttgccgc tgtcactcat caggcgaatc gttgcgggtg
    19561 tacctgagcg aatatttttc agatcggttt cgcggaagtt ggcgatcacg taccagtgac
    19621 gagtgtcgat tagggtaaaa atagggcgca tggcagaagc aaattgcccg acggaggttt
    19681 tgagggaaat gacccggcca tcaaacggcg cgcgaacggt cgccatttcc agatgaagtt
    19741 tggtcagggc aatatccgct tcgaccgccg cacgttgcgc caccagcgca tcgacgccgc
    19801 tgacggcgct ggcggctgac tgcgcctgta acaatacggc attaagatcg gcttctgcgg
    19861 cgcgctgcgc agttcttgca cggtcaacgt cttccgctga gacaaaaccc tctttcagta
    19921 atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc ggcggcacgg gctttttcta
    19981 ccgtggcatt aatcgagtcg gcaccaaact gttgcgcatc aacgctacgc tgggtgagca
    20041 taatttgctt atccagcgcc gcgagggagg cttcagcttt cgccagattg gcttcgtacg
    20101 ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac tgcctggttg tcggtgaccg
    20161 ccagttctac aatgcggccg ctgacttccg gcaccacatc aatggtatct gctgacgcgt
    20221 aagcgtcatt agttgatggc gcactgtcta cgcgccagat aacgaaaaca agggcaacca
    20281 gcgccaatgc aaccactaac agagcaggga atttactgcg aggagctttt ttcggcgtac
    20341 tttccatgag cgcgtctcaa atagattaga aaaatgccag ccagaacagc atggcgtaga
    20401 gagcaaaaag ggcggtataa ataattccga caaatgccag attgatattt gcccgctgaa
    20461 taactcgcct cgtgatgagc atcaaaataa ggctggcaat ggcgcagaaa aaccagctgg
    20521 gataataagc accgatcacc gggattgccg gagataatga gcatcccgaa accatgaaga
    20581 cgggaattat ccaggctgtt tttttgagtt gcatcgccat gcgagagaga actgttggca
    20641 tattaaatac atcctgtctg ctgcgaacaa tatctgtagt gaatatcaat atccattgaa
    20701 ggcgtcagta tggctatttt ctcgatgaat aaaaatagct ttattcagcc accgcattca
    20761 taact
//
LOCUS       DAZFWP020000084        20714 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFWP020000084 DAZFWP020000000
VERSION     DAZFWP020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.084"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..560)
                     /locus_tag="ACHK0Z_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             complement(<1..560)
                     /locus_tag="ACHK0Z_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4502936.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALESAGCELIFEDKASGKKAERPG
                     LKKVLRMLSRGDTLVVWKLDRLGRSMRHLVVLVEELRDRGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLDAARAEGRIGGRRPKYQEETWQQMRRLLENGIP
                     RKQVAIIYDVAVSTLYKKFPASSFQS"
     gene            666..992
                     /locus_tag="ACHK0Z_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             666..992
                     /locus_tag="ACHK0Z_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HGU4502937.1"
                     /translation="MFADYENLAVVVITSLLSGTGVFLLGVRDGRISASLLNLASELF
                     TAVTAGLAGYGVAVSQEWPEGIIFCVVLIASNNGSEILQGLKSRASNVLNLLSVIANG
                     GKGGEK"
     gene            992..1438
                     /locus_tag="ACHK0Z_RS14770"
                     /old_locus_tag="pgaptmp_002954"
     CDS             992..1438
                     /locus_tag="ACHK0Z_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4502938.1"
                     /translation="MGNFGNYFIFIMAFVAIIDRFIFCRKSVEILSIGDAVVKESAIA
                     FPVSLRVKRSWVSNAQIEYWLQDLKNPSVVISGKTRPVDASKRGEREEYLLIDTKYLE
                     PTKWDLKVTLTNGNCRLNPLYRIFPINDCIERQFTIEKREGDWDVK"
     gene            1428..2048
                     /locus_tag="ACHK0Z_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             1428..2048
                     /locus_tag="ACHK0Z_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502939.1"
                     /translation="MSNKNNYVLLNYDELNEKGLAKLVKEISKGGYKIARVIPASNGR
                     KKDGIMTRTFTLIGIDEQTMEVQVNDTGDISGIKLNGKTVPFKPVKTMSALGQSLAAL
                     FNRGATSFQKSLARKLARAAKNIDDGNKKRQGVKSNAQKLAEAKESRDALREDIANAN
                     DKLSKLQSKSDKVMQDTGNVKAALSQEVAKTRALKEEIARLEDEHD"
     gene            2041..3978
                     /locus_tag="ACHK0Z_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             2041..3978
                     /locus_tag="ACHK0Z_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head protein"
                     /protein_id="HGU4502940.1"
                     /translation="MINNPLRINIQSTFADTIPGYMPSRWSSEELNEDDLIFEAATLE
                     DIELAYMGNEILVVSDEAMFESITTTRIRLAQTMRAFVRALNRGLNGTNIMAGTDEAG
                     TDENGRNCIGGAIIGRVRRVANIPVLTAQIPLTDGQSTSIIFHSPTADGAKIKSNDEL
                     VAFQFLLNKRNVTHVVAPIGGRDVSLSQVCQALSNLIERNSEKFKKAKERQDKMKADI
                     ESYLNEADKLAKERSIMIDQVDVAQRGLTEKRAALTEMQKKLDAQKAINEELQAKRDK
                     LLSAKGEKTKERAFSDQLRHVKHNLAIDGKTVLDNGAEVNYVTSGSDNFVTIVAPEGK
                     FSIDASAVKGGSLADAATKLLKAYREHNADKYKVDVLPGQQEDSEPSQTDPETNPVSQ
                     PESQDVGKYHYALQSRPAGVGAVPDGNKAVLDRPDQADQYYEYARHGIITYDRKLTDE
                     EVSQYELRYLPDDDELKNMARELVASSMAEHIDGYVDLFGSDLKTFKAQVKILFRKEF
                     PNVAYPLGDGENLFIMDVYNALQNHSNEATPEPELAPVVEPESHPQTAPEQQPTPEDA
                     EEAATEADQEADKALEYLKSVPEQFTSRDLTVISAELDHVQEAANALISAGRYDENEA
                     TVGAAVDYLINILAEIQQGGA"
     gene            3978..4346
                     /locus_tag="ACHK0Z_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             3978..4346
                     /locus_tag="ACHK0Z_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502941.1"
                     /translation="MTISVLDRLKLGKELSDLMQAQKTAPVLQRVAIGKQIVDVMLKL
                     GLGTAAQPAAEPQQQTDPVVDEVPKIVTDFLGGVFTKSTQMEFIDALRGISNYVGEFL
                     TLEQAKEQTISWVKANGYAG"
     gene            4441..5826
                     /locus_tag="ACHK0Z_RS14790"
                     /old_locus_tag="pgaptmp_002958"
     CDS             4441..5826
                     /locus_tag="ACHK0Z_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502942.1"
                     /translation="MNKSVTSALSGAADINSVIALVSSLERKETRLGRSSYVVTSKGA
                     EVKTAFKVVDASSLIISNNLDGTINPAFPEELQPRDRTRLSSKLQVNRIASNLRPAQL
                     TDSGMSSHGAPIVGPDNVVESGNGRSMGIWRAYEQGQADEYRQYLIDHAKEFGLNPDE
                     ISQMSMPVLVRERLTDVDRAQFARDSNISDLQEMAASEKAYADAQFLTESVMALFNPS
                     DDGNLLARSNDAFIRAFLREIGDTATAGLLTADGRPTKQLIDRIQNAIFAKAYKDERL
                     VRLVSEEPDPEMRNILTALNTAASDFAQMQSLSGDAHHDAVTGLVDGIEQVNGLDTQA
                     IAALQEAINLVREAKDNGQAVEEVIAQRGLFGDSTPEAEALALFIVANNRSAKRMGAA
                     FKKMAQKINDELTHKQQALGDMFGGGDVDLRRILSAVSDEIEGEFGEGKGLNFAMFEN
                     STDLNNCRTEN"
     gene            5891..7573
                     /locus_tag="ACHK0Z_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     CDS             5891..7573
                     /locus_tag="ACHK0Z_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4502943.1"
                     /translation="MATKDKNKGFLSALKKAFNGGDVTPTDPVVFTSGHSVVARSGLS
                     ALRPGILGSNSDGMTSAADSISLSAELPGERLQKYNILETMAKSPTISTALNIHIAHA
                     LAPSKKTGQAFILSPKDGSDAEAVSRCEELTADLGAMINDGLPSWAMIMAIFGVSYVR
                     PYAEQGRGITGIESSYYTLPHFVQEFYRGSQLVGFSGDYILDTHSLRRVITEPWNLVS
                     MKNPYWTPQHKVIPVSYGTKGYSLLSDQADKPLMETQNYGTSFLEYSYEPYLNLCASL
                     AALKSTRNNAAKIDRLIALTTNTLDPVNAANYTRGVSQALKRHSDLVAQRSINANAIP
                     TVLNHLIPVMGDGKNGITIDTQSIPADISGIEDVMFHLRQLAASLGIDATMLGWADQM
                     SGGLGEGGWQQTAIQAALRANWIRQAAQRTIYRLHDIHLAYKYGKVYTEKNRPYDVQF
                     NSMNTAIQEEENRELDARANFVAVISQIMDQVQNNPKLAGSDAFMRYLFTEQLHIDDD
                     TLNTMIKEFKANESEQNNEHGMYESAPLSSGDDPENWTPEQLINFAKFVMSN"
     gene            7587..8585
                     /locus_tag="ACHK0Z_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     CDS             7587..8585
                     /locus_tag="ACHK0Z_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HGU4502944.1"
                     /translation="MQSLNTVTDRFSLVEKIRKHTPQNNRNYVIQSVRDTFNSPETKE
                     RIALGEMYGYYGHGRRAMHYNKTKSLNLPEVSVVMVDGKPVVLENVPSNRTIDISIDD
                     NGIVTHTQEILDTDTGRIVQGMINSGAGGWSWATSGPDSSVSLVKSFHGFDYVTVPNY
                     ISLDKKSLMLESAEERDAAIHAALIEQGFTDNAATDLVHHFSAMKNQQAMLESAHREA
                     LESELMLLEVENIQLRDKLRAQTAMMESQGENAKQCRRILRDAIQEMPVFISAEQRRA
                     LCRMQSEDDARIVAAMLESLGANATYGLPVSGKKEPELSPKENKNTTPLLFVSRRG"
     gene            8786..9748
                     /locus_tag="ACHK0Z_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             8786..9748
                     /locus_tag="ACHK0Z_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic replication protein"
                     /protein_id="HGU4502945.1"
                     /translation="MSILNSVSVTFNTSEYKNNTEYHKKIKGHQLSFFARHSGLISPS
                     HSAIIAEFANLAGATDEYMIRRSYADMSEITGRSISTVRRAFAEATKCGMLVKQHQVA
                     NNNAQVCNVYRFTTQFLHFIHVAMEIGGKQGIKFANATKLVKQLISKVRYFFETGNPL
                     FKLNKSPHVQNEQPIENKSHSIAKRRERSCAVQPKASPADSSQTDNGVLDKKPVIREE
                     HTNHCLAAAKARAAKRRSDEGHAKRQALYRTAEKLAKKFAWIRSAATAVNKPKQSSAL
                     DFSMDYSGSQGCATIGEAFDLMKQRGYRSEFDREDWSIPAGFRG"
     gene            10162..10386
                     /locus_tag="ACHK0Z_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             10162..10386
                     /locus_tag="ACHK0Z_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4502946.1"
                     /translation="MVNANPCSRPEFIWRFHSLQKHCDHFAIAATEKEARSLIPDQHG
                     VLTGRFSTSPQPNTNPWYRNITNPGIAEAH"
     gene            10386..11093
                     /locus_tag="ACHK0Z_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             10386..11093
                     /locus_tag="ACHK0Z_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4502947.1"
                     /translation="MDICILEKPSMTSIEIAELVGSRPDNVKISIERLAKSGVIQLPA
                     LQVFEKINNLGLRRSVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEEQARQ
                     PLTEIEMIAAMAANAVQQQKRLHVVESKVSQVVETVEQIKKGNMPDGYIGYRQLAAKC
                     GLTEAKCRNLVNAYRIPTDTHEFLTPEGVLSRRSIVALSPFMNAFNRMMSEAEHRGKR
                     WYHPKMGQFQVIGWGGE"
     gene            11093..11290
                     /locus_tag="ACHK0Z_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             11093..11290
                     /locus_tag="ACHK0Z_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502948.1"
                     /translation="MDIMQAVKKIREGQADVCRRKAWSVGVWAWPDNYSTTGWFCING
                     LQIREVDLSTADIVGNDWIVT"
     gene            complement(11323..11808)
                     /locus_tag="ACHK0Z_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             complement(11323..11808)
                     /locus_tag="ACHK0Z_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HGU4502949.1"
                     /translation="MANIEPRWLIEARKHIGLTEIKGAKHNPEIVQFWRDIKRGGIKD
                     DETPWCAAFVGAMLERVGIRSTRFESAKSYLDWGEKLDTPAYGCIVVFTRVGGGHVGF
                     VVGRRANGDLLVLGGNQGDAVNIRAFPTSRVSGYRWPAGEPRNTALLPVGDAATSTNE
                     A"
     gene            11960..13300
                     /locus_tag="ACHK0Z_RS14830"
                     /old_locus_tag="pgaptmp_002966"
     CDS             11960..13300
                     /locus_tag="ACHK0Z_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4502950.1"
                     /translation="MDKLLHSISEASSLGELIEIVSRLHKLRAVATFGVRKADGVSIQ
                     KTRRAANNAAVNLLNSLPPGFDGAKLTDEQRQILAGYTGEGGLTDGEGSQYEYYTPQF
                     MAEGIWDLFADYGIDGGHVLEPSAGTGIFQETKRQGMIMTSAELSPISGRINQLLHPE
                     DDVNIGAFEALAAKDAMYDHAVGNVPFGEGRSGVAGLDPAYANEKNVGNYFVLRTIDK
                     VKPGGLIVLVVPNGMTDGAKYKKLRDKVSRKAEFLGAHRMPSGTFSESGTDTVVDVWV
                     LRKHPETFLEMIPDTDDSTLKSANVLWDTFLKGKWFTTEGKRFVYGDMERTSFRNTLV
                     VKKDGRVSNESMKTALSRRFDSRINWDLLGVTTQAWQGAKVGDKRLVGGIWHEFDGLK
                     WVKDTTTKSSALDVSRYGVSTFGDLHRVPVNKRDSGFVMGPDIGDRVRLSIGCQ"
     gene            13245..18794
                     /locus_tag="ACHK0Z_RS14835"
                     /old_locus_tag="pgaptmp_002967"
     CDS             13245..18794
                     /locus_tag="ACHK0Z_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HGU4502951.1"
                     /translation="MSWDQISAIASDYPSVVSDEVAAMIRFAGKQREKDRERVMRGAL
                     IGQLINKALDLRNLGVNVDDKLADASRLAAAEVAKYGPPHAIKLNGLAEAGAKNWMTF
                     TGNVKQDGSTSDLLAGRLDVTDGAAGIDFTRPEQVIAHLFSDVALNPITLGDFREAFA
                     GELPEDDDAALEYLAKFDDIAIDGYGCLLPMDRATSGDIATKTALLMGWRDASTGEQK
                     ANFERQLAKIEEKRIFTPLNKVTVNLNARWLDRRLIKEFLAEQGYDEFKYTEPDLKVV
                     DGILVSPDDYEGKDGVFTGYQLRTVNGKNGNEFKKANNKDGFLNQLENYLNGVKPRGQ
                     NANEYLDKIAQLEVSFNDWLRTHPQADQIAREYNDAFNGFIPFTHSDAPLGLEGLSGK
                     RIPLTYQNEEVRRLSEDGRGIMGFGTGLGKTTTALALEAYNFETGRTKRTCIVVPKAV
                     YQNWYHEAQSFYSADAFANMMFIGLDEVRDDSGNILTAPVLDENGEPRLDSNGQPLTR
                     NVVKESASAVIVQRMNMIPSSNWRTVIMTKEQFASIPLREKTIEENSQQAVFNAVEMG
                     RLDLASGKHRDAQKKNKIKDQAANTGTTKKQNIPYFEDMNFDSVIADEGHNYRNSFNA
                     GREAGQLAYLPNPAVSKMARDMAVKAAYMMKRNNGRGVVMLTATPLVNSPIDAFNMLS
                     TVIPQEEWMRMGIITPDDFVRVFGKTATVQVQKISGEVEEKQGLVGFQNLDGLRGIFH
                     RWTTLKTAADVGATVKIPDIIENTLQIPMTGEQETAYEELRKRAQELSRNDELTVDEN
                     GHITNDKPDDFIFSIIRDMDKVAIDPDLYASAITFQFPVELADQVKAIADALPKVAGG
                     KVADDVDEAEEDGAGGLVSTRTSKVVKTAFSEHPTHVEIRASIELEAEILKAIAAAGI
                     DMQQVSHPIPPKYAALIENLREGLKNGKQIVFIDEKAQHQKLRRIIASALQMPEQEIG
                     IINATTVSQAGGIKLKKVNKPTEPTPNKNGEYKEGAWETYYSKLAQYEDYLSAKNDAG
                     LEGMEGIAADYNEGRTRIIICNKKAEVGINLHIGTTDIHHLTLPWTPASIDQRNGRGA
                     RVGSPQEKVNVHYYCGKGTFDDFRLDTLKRKKDWIKMVMTSDMSEIANGDADDADERA
                     IMLAANPEERRAIMARQAQEREERLKLKAQREANNALDIYLKAANAAGKDIGMMETEL
                     KSAMEQIEYYQRNLDDLIKAGTNRNGQKYALEQLSSYRKRARVLRFAITRAKDADTIM
                     KRSRGDVERAIKSGVLDISIDVIQNPQEYMRTDKNILLHKGSYYRAVIDDRETTAIVR
                     VEKIDGEKAQYLCRIAWLDRASRYARAPGSIVTLPFDSVIEAVTFEEGVAESRATAAR
                     GVQCHQLSTILTRDQFYDAIRNGVMNVIAGKPGNAWGGGSAIKYWAYRTDDGQIALSS
                     TYDGMIGQKIGRTSDIAPEQWIYPDINNEALKREVAKIQASDNPLNIVDGEGFLQALY
                     GYDYIQGMQAWGAQATMDDVAQEFENWMAGRGLSGRSLNKVTSEDILSLFRKPSGEGL
                     ESFWRTVLAYRLFQGRMKAFSNIDDIERMFNQVKIAKIKAKIEKTRSALVAWRDALFE
                     QYKSLSDADGWAALEQLANNGVDGAKLAIVQADNPLESPAAQWIKVGAVINAFDEKHI
                     SKDDFADAVSVNRLVEMATRRARELKDGDYVNELPDALKSATWQDYVSLKNGGATESE
                     IAKRLEQAESKREEQAAVAEARADEAANDDYIIVVNDKPIRAKARVRGRWWSVSEDVG
                     AVYLIADQPGSSTIRNAKDAIKKIGGRFWNFEANPVADVNFDRPAWMVSTRYSVDELR
                     KIIADAA"
     gene            18831..19265
                     /locus_tag="ACHK0Z_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             18831..19265
                     /locus_tag="ACHK0Z_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="olxA"
                     /protein_id="HGU4502952.1"
                     /translation="MTTLIDTVKPTESYLEDILPQALNGRSEEEYLTVYLDGMVERLK
                     KNPQLYHLYGPWWPALKTILLERGDISFGQVVDSDVAEIYKMSRPALTVLAGHLYSSD
                     RLENDAVYNPVHALEVAPYADDTEPYVYISYDESIEKYRIMG"
     gene            19268..19528
                     /locus_tag="ACHK0Z_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             19268..19528
                     /locus_tag="ACHK0Z_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502953.1"
                     /translation="MRKNRRFTVEDLKEYSISKGYVLEFHRYKKVFTLRKAENPANWS
                     WIYFPHTDDKLVELVDDLTYEGWLIAIDKTIKELSEQDKITL"
     gene            19668..20159
                     /locus_tag="ACHK0Z_RS14850"
                     /old_locus_tag="pgaptmp_002970"
     CDS             19668..20159
                     /locus_tag="ACHK0Z_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /inference="COORDINATES: protein motif:HMM:NF020354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGU4502954.1"
                     /translation="MLLFRIFTINGCVRKIARKRRRRSEYSETSPHAAALRELEKNPD
                     LIKRNHEHYEQVRVLDWLYRNMRYVYEHTHATPNGGLRGIRTAIKMVAEGQKKGYPDL
                     SIDLACGGYHGMRIEMKHGRNRLTPEQLVWMTRLTEAGYYCFEARSAAEAIKAITEYV
                     CLD"
BASE COUNT         5744 a         4586 c         5405 g         4979 t
ORIGIN      
        1 taggattgaa atgatgacgc aggaaacttt ttataaagcg tggaaacagc aacatcatag
       61 atgattgcaa cctgcttacg ggggatgcca ttctccagca atcgacgcat ttgctgccat
      121 gtttcttctt ggtatttagg ccgacggcct cctattcgac cttctgcgcg agctgcatca
      181 agtccagcgc gtgtacgttc aacgataagc tcacgttcca tttctgccag cgcccccatt
      241 acgtgaaaga aaaagcgtcc cattggtgta ctggtgtcga tggagtcagt gagactccgg
      301 aagttaatgc ctctgtcacg cagctcttcc accagcacaa ctaagtgacg catgctgcgc
      361 ccaagacgat ctaacttcca tacaaccagg gtgtcacctc tggaaagcat acggagaacc
      421 ttttttaacc cagggcgttc agcctttttg ccgctcgcct tgtcctcaaa aattagctca
      481 catcctgcgc tttcaagggc gtttcgttgt aaagctgtgt tttggtcatt agttgatacg
      541 cgaatgtatc caataagcat atgccccccc ttgtatgtta aggggggaga ttttagaaga
      601 tgttctgtaa ggaaataaca aactgactaa gttagtggcg cattagatgt tatttctgga
      661 ggggtatgtt tgcagattat gaaaatctgg ctgtagtagt cataacatcc ctactaagtg
      721 gaaccggtgt attccttctc ggagttagag atgggcgcat ttctgcgtcc ctgctcaatc
      781 ttgcgagtga attgtttact gcggtaacag ccgggcttgc ggggtatggg gtggcggtta
      841 gccaagaatg gcctgaaggt atcattttct gcgttgttct gattgccagt aacaacggta
      901 gtgaaatttt acagggcttg aagtccagag ctagtaacgt cttgaatctc ttaagcgtaa
      961 tagcgaatgg gggaaaaggt ggagagaaat aatgggtaac tttggaaatt atttcatttt
     1021 tatcatggca tttgttgcca tcattgaccg tttcattttt tgtcgcaaga gtgttgagat
     1081 tttgagtatt ggtgacgctg tagtgaaaga atccgctata gcatttcctg tttcgctacg
     1141 tgttaagcga agctgggtca gcaatgctca aatcgaatat tggttgcaag acctgaaaaa
     1201 tccatctgtg gttatttcag ggaaaacgcg accagttgac gcctcaaaac gcggtgagag
     1261 agaagagtat ttgctcatcg atacgaaata tcttgagcca acgaagtggg atctgaaagt
     1321 cacattgact aacggtaact gccgactgaa tccgctttat cgcattttcc ctatcaatga
     1381 ctgcatagag cgccagttta cgatcgaaaa gcgtgaagga gactgggatg tcaaataaga
     1441 ataattatgt tcttcttaat tatgatgagt taaacgaaaa ggggctggca aaactcgtca
     1501 aagaaatcag caaaggtggt tacaagatag cgagggtgat ccctgccagt aatggcagga
     1561 agaaagatgg gatcatgacg cgcacgttta cccttattgg tattgatgaa cagacaatgg
     1621 aagttcaggt taacgatacc ggtgatatat ctgggatcaa actgaatggt aaaacagtac
     1681 cgttcaagcc agtaaaaaca atgtcggctt tggggcaatc tcttgcggct ttatttaatc
     1741 gcggagcaac ttctttccag aagtcattag ctcgaaaact tgctcgtgca gcaaaaaata
     1801 ttgatgacgg aaataagaag cggcagggag ttaaatctaa cgctcagaaa ttagctgaag
     1861 cgaaagaatc acgtgatgca ctccgtgaag atatcgctaa tgctaacgac aaactgtcta
     1921 aactccagag caagtcagac aaagtaatgc aagatacggg taacgttaaa gctgccttgt
     1981 ctcaggaagt agctaagaca cgtgctctta aagaagaaat cgcgcgcctg gaggatgagc
     2041 atgattaaca atcctttacg aattaatatt cagtctactt ttgctgatac gatccccggc
     2101 tatatgccaa gtcgctggtc ttcagaagaa ttaaacgaag atgacctgat ttttgaggcg
     2161 gcgaccttag aagatattga gcttgcctat atgggcaatg aaattctggt tgtgtctgac
     2221 gaagctatgt ttgagtccat aactacaacc agaattcggt tagctcaaac aatgcgagct
     2281 ttcgtccgtg cgctgaatcg tggcctgaat ggtacgaata ttatggcagg gactgatgaa
     2341 gctgggaccg atgaaaatgg acgtaattgt attggtggtg cgattatcgg tcgtgtacgt
     2401 cgggtagcta atatccctgt tttgaccgcg cagataccgc ttactgacgg gcagagtaca
     2461 agcataattt tccactctcc aacagctgac ggggcaaaaa taaagagtaa cgacgagctt
     2521 gttgcatttc agtttttact taataagcgt aacgtaactc acgttgtcgc cccgattggt
     2581 gggagagatg tttctttaag ccaggtatgc caggcgctat ctaatttgat tgagcgtaac
     2641 agcgaaaaat ttaagaaagc aaaagagcgc caggacaaaa tgaaggcaga tattgagagt
     2701 tatctcaatg aagcagataa actggcaaaa gaacggtcaa taatgattga tcaggtggac
     2761 gttgcgcaac gtgggctgac tgaaaagcgt gctgctctta cagagatgca aaaaaaactt
     2821 gatgcccaga aagccatcaa cgaggaatta caagcaaaac gcgataaatt gttgtctgct
     2881 aaaggtgaaa aaacgaaaga gcgagcgttt agtgaccagc tgcgacatgt aaaacataat
     2941 cttgccattg acggaaaaac agtgctggat aatggcgctg aagttaatta tgtcacgtcc
     3001 ggcagtgaca atttcgttac gatagtagca cctgaaggta aattcagtat tgatgccagt
     3061 gctgtcaaag gaggcagttt ggcagatgct gcaaccaaat tacttaaagc ctaccgagag
     3121 cataacgcag ataaatataa agttgatgtg ttaccaggcc agcaggaaga ctccgaacca
     3181 tcacagacag accccgagac gaacccagta tcacaaccag agagccaaga tgtaggtaaa
     3241 taccactatg cacttcaatc tcgcccggct ggtgttggtg ctgtgccgga tggaaataaa
     3301 gccgtgcttg atcgtcctga ccaggctgat cagtattacg aatatgctcg tcacggtatc
     3361 attacgtatg accgtaaact gacagatgaa gaagtcagtc agtatgaatt acgctatcta
     3421 cctgatgacg atgaactgaa aaatatggcg agggaactgg ttgcgtccag catggcagaa
     3481 cacattgatg gctatgtaga cctgtttggc agtgacttga aaacattcaa ggcgcaagta
     3541 aaaattcttt tccgcaaaga gtttccgaac gtggcttatc cgcttggtga tggggagaac
     3601 cttttcatta tggacgtata taacgccctt caaaatcatt caaacgaagc aacgccagag
     3661 cctgaattag cacctgtagt tgagccagaa tcacacccac aaacagcacc agagcaacag
     3721 ccaacgccgg aagacgctga agaagcggca actgaagctg atcaggaagc agataaagcg
     3781 ctggagtacc tgaaatccgt gccggagcag tttacatccc gcgatctgac agtcatcagt
     3841 gctgaacttg atcatgtgca ggaagcagca aacgctctaa tcagtgcagg tcgatacgac
     3901 gagaacgaag ccactgtggg tgcagcagtt gactacctga tcaacattct ggctgaaatt
     3961 cagcagggag gtgcttaatg accatctctg ttttagaccg cctgaaatta ggtaaagagc
     4021 tatcagatct aatgcaggcg cagaaaactg cgccagtatt gcaacgcgtc gccataggta
     4081 agcagattgt tgatgtgatg ctgaaattgg gcctggggac tgccgcgcaa ccggcagcgg
     4141 aaccgcaaca acagactgat cctgttgtag atgaagtccc taagatagtg actgacttcc
     4201 tgggcggtgt gttcacaaag tccacacaaa tggaatttat tgatgcgctg cgtgggatct
     4261 caaactatgt tggcgagttc cttacgttgg agcaggctaa agagcagacc ataagctggg
     4321 tgaaagctaa cggttatgcc gggtaatcaa aaggggctga aatgcccctt tcacttatct
     4381 gttctcacat aaatcacaca ctttttacac tcacagcttt ctctatagtt ggagtgtatt
     4441 ttgaacaaat cagttacttc tgcgctttcc ggggccgcag atattaacag cgtcattgct
     4501 ctggtttctt cattagagag gaaagaaaca cgcctggggc gaagtagcta cgtagtcacc
     4561 agcaaaggtg cagaggtaaa gacggctttt aaggtcgttg atgccagcag tctgatcatt
     4621 tcgaacaacc ttgacggtac gattaacccg gccttcccgg aagaactcca accacgagat
     4681 cgtacccggc tatccagcaa actccaggtt aaccgtattg cctccaattt acgcccggca
     4741 caattaacgg attccggtat gagcagccac ggagcgccga tagttggtcc ggacaacgtt
     4801 gttgaatccg gcaacggaag gagtatgggt atctggcgtg cctatgagca aggacaggcg
     4861 gatgagtatc gccagtacct gatcgaccat gcgaaagagt ttggtctgaa tcctgacgaa
     4921 atttcgcaaa tgtctatgcc cgtactggtg cgagaaagac tcacagatgt agaccgcgct
     4981 caatttgccc gcgattcaaa cattagcgat ctgcaagaga tggcagcaag tgaaaaagcg
     5041 tatgcggatg cgcaatttct cactgagagc gtcatggcgc tatttaatcc ttcagatgat
     5101 ggaaatctgc tggcgcggtc caatgatgcg tttattcgtg cgtttttgcg cgaaattggc
     5161 gatacagcga cggcaggctt gcttactgcc gatgggcgtc ccacgaaaca gcttatcgat
     5221 cgcatccaga atgcaatttt tgccaaagcg tacaaggatg aaaggcttgt tcggctggtg
     5281 tcggaagagc cagatccgga aatgcgtaat atcctgaccg cattaaatac ggcagccagc
     5341 gattttgcgc aaatgcagtc gctgtctggc gacgctcacc atgacgcagt aacaggctta
     5401 gtggacggta tagagcaggt taatggcctg gatacacagg cgatagcagc actccaggaa
     5461 gcaattaatc ttgtacgtga agccaaagac aacggtcagg ccgttgaaga agtgatcgcc
     5521 caaagggggt tattcggcga cagtacaccg gaagctgagg cattggcgtt attcatcgta
     5581 gcaaataatc gcagtgcgaa gcgtatgggg gcagcattca aaaaaatggc gcaaaaaatc
     5641 aacgacgagc tgactcataa acaacaggca ttgggcgata tgttcggcgg tggcgatgtt
     5701 gatctgcgtc gtattttatc tgctgtatct gatgaaattg agggtgaatt tggcgaaggg
     5761 aaaggtctga actttgctat gtttgaaaat tctacagatc taaataattg ccgtacggaa
     5821 aactaaacca accgctgaaa tcacatactg attatgctgc cgcaaaactg gaaacaattt
     5881 gcggtgattc atggcaacga aagacaaaaa caaaggcttt ctgtcagcat tgaagaaagc
     5941 cttcaacggt ggtgatgtta cgcccaccga tccggtcgtt tttacgagcg ggcatagtgt
     6001 cgttgctcgt tccggcctgt ctgctttacg tccaggtata ctggggagca acagcgatgg
     6061 catgacgagt gcagcagatt caatttcttt atccgctgaa ctgcccggcg agcgcctgca
     6121 aaagtacaac atcctggaaa cgatggctaa aagcccgact atctcgacgg ctttaaacat
     6181 tcacattgca catgcgcttg cgccttcaaa aaaaaccgga caagcattca tcctttcgcc
     6241 aaaggatggt tccgatgccg aggcagtaag tagatgtgaa gagttaaccg ctgatttggg
     6301 ggcgatgatt aatgacggac ttccttcatg ggccatgatt atggctatct ttggggtgtc
     6361 ttatgtccga ccttatgctg aacagggcag ggggataaca ggcattgagt ccagttatta
     6421 cactctgcca cacttcgtcc aggagtttta tcgtggtagc cagctggtag gtttcagtgg
     6481 tgattacatt ctggatacgc atagtttgcg cagagtaata accgaaccgt ggaatctggt
     6541 ttccatgaaa aatccctatt ggacgccaca gcataaagtt attccggtta gctacggcac
     6601 aaaagggtac tcactgttaa gtgatcaggc tgataagccg ttaatggaga cgcagaatta
     6661 cggaacttca ttcctggaat acagctatga accttacctt aatttatgtg cttcgctcgc
     6721 cgcgctgaaa tcaacgcgaa ataatgcagc caaaattgac cgcctgattg ctcttacgac
     6781 gaacacactg gacccggtta atgcggcgaa ctatacgcgt ggcgtcagcc aggcattgaa
     6841 gcgtcatagt gacctggtgg cgcaaaggtc gattaatgcc aacgctattc cgactgtgct
     6901 taaccatctg atacctgtta tgggagatgg gaagaacggc atcactattg atacgcagtc
     6961 tatacccgca gatatcagcg gcattgaaga cgttatgttt catcttcgtc agttagcggc
     7021 cagtttaggc attgacgcca cgatgctcgg ctgggcagat caaatgagcg gtgggcttgg
     7081 agagggcgga tggcaacaaa cagctatcca ggccgcatta cgtgcgaact ggatcagaca
     7141 ggccgcccag cgaactattt atcggcttca tgatattcac ctcgcttata aatacgggaa
     7201 ggtttacacc gaaaaaaacc gtccttatga cgtgcaattc aactcaatga acactgctat
     7261 ccaggaagag gaaaaccgag aactggatgc acgcgccaac tttgttgctg tcatctccca
     7321 aattatggac caggtccaga acaacccgaa actggctggc agcgacgcgt ttatgcgcta
     7381 tctgttcacc gaacagctgc acatcgacga tgacactctc aataccatga tcaaagagtt
     7441 taaggcgaat gaatcagagc aaaacaacga gcatggaatg tatgaatccg cgccgttgtc
     7501 gtccggtgac gatccggaaa actggacgcc agaacagtta ataaactttg caaaatttgt
     7561 gatgagcaat taaccggagg gaaataatgc aatcactgaa tactgttacg gatcgcttct
     7621 ccctggtaga gaaaatccgc aaacacacac cgcagaacaa ccgaaattac gtcattcagt
     7681 cggtacgaga cacattcaac tcgccagaaa caaaagagcg tatcgcgctg ggtgagatgt
     7741 atggttatta cggtcatggt cgccgggcga tgcactataa caaaacgaaa agcctgaact
     7801 tgccggaagt ctctgttgtg atggtagatg ggaagcctgt tgtattggag aacgtgccgt
     7861 caaacaggac tatcgacatt tcgattgacg acaacggcat tgttacgcat acgcaggaaa
     7921 tactggatac agacaccggg cgtattgttc agggcatgat taattctggt gctggtggtt
     7981 ggtcgtgggc tacatctggt ccagattcat cggtatccct ggtaaagtct ttccacggct
     8041 ttgattacgt aactgtgcct aactatatca gcctcgacaa gaaaagcctg atgcttgaat
     8101 cagcagaaga gcgcgatgcg gctattcatg cagcacttat cgaacaaggt tttaccgata
     8161 acgctgctac ggatctggtt caccactttt cagcaatgaa aaatcagcaa gccatgctcg
     8221 aatctgcaca tcgtgaagcc ctggaatcag aattgatgct ccttgaagtg gaaaacatac
     8281 agcttcgaga taagctgcgc gctcaaacag cgatgatgga aagccagggg gaaaatgcaa
     8341 agcaatgccg ccggattttg cgtgatgcta tccaggaaat gcctgttttt atcagcgcag
     8401 aacagcgccg ggcgctctgc cgtatgcagt ctgaagatga tgcccgcata gtggctgcga
     8461 tgcttgaatc tcttggggca aatgctactt atggcttacc ggtatcaggc aagaaagaac
     8521 cagagttatc accgaaggag aacaaaaaca ccaccccgtt actcttcgtt tccagaaggg
     8581 ggtaatcgcc ttgttaagtt aaatttctaa catagtgtat ttatatacac tgttgatcct
     8641 agtggatggg atcgagtagg atcgcggtca ctgctccggt agcgactgcc aaaaaagcaa
     8701 aagccgctga gaagcggctt tttggatgta taaatccact gccatagatt tatacggtag
     8761 atagattttt tcacggaact atctggtgag tattttaaat tcagtttcag taactttcaa
     8821 cacttctgag tataaaaaca acactgaata ccacaaaaaa atcaaaggtc atcagttgag
     8881 tttttttgct cgtcatagcg gtcttatttc cccttcacat agcgccatta ttgccgagtt
     8941 cgccaacctg gcaggggcta ccgatgaata catgatccgt cgctcatatg ctgatatgtc
     9001 cgagattaca ggacgcagta tttctactgt gcgccgggca tttgctgaag cgacgaagtg
     9061 cggaatgctt gttaagcaac atcaggtagc gaataacaat gcccaggtgt gcaacgtata
     9121 tcggtttacg acacaatttc tccacttcat tcatgtagca atggagatag ggggtaagca
     9181 gggcatcaaa tttgccaacg ccacaaaact ggttaagcag ttgatttcaa aggttcgtta
     9241 tttttttgaa actgggaacc ccctgttcaa attgaacaag tccccccatg ttcaaaatga
     9301 acagccaata gaaaataaga gtcactctat agccaaaaga agagaaagat catgcgcggt
     9361 tcagccaaag gcttcacctg cagatagttc tcaaacggat aacggggtac tggataaaaa
     9421 accagtaatc agggaagaac ataccaacca ctgcctggcg gcggcaaaag cgagagctgc
     9481 aaagcgtcgt tcagacgagg ggcacgcaaa acggcaggct ctgtatcgta ccgctgaaaa
     9541 gctggcaaaa aaatttgcat ggatacgtag tgccgctacc gcagtgaata agcctaaaca
     9601 atccagtgcc ctggatttct caatggatta ttctggctcc caggggtgtg caaccattgg
     9661 tgaagccttt gacctgatga aacagcgtgg ctatcggtct gaatttgacc gtgaggattg
     9721 gtcaatccct gcgggattta ggggatgacc tgtgtgtcta caaaacatcc caatgatcgg
     9781 aaaatgctcg gccgatgatc tatgttagaa aattaatttg ctgtttctaa aacggaatgt
     9841 atgatctgca tcatcgactg aagtcatcca ttgctgaaat ttttagttga tcttccatgt
     9901 tttcaggctt atattcatgc cgtcgtagca aattctgcga ccgggtttga cagcctgaat
     9961 gttagtgcgg acaaccgcag atttccgata ttgcggtatt tttgtgtccg taaaccgcgt
    10021 tacgcccgaa ttatggtggg gcgtgatggg gaggcttcgg cctgctggtt tcactaacgc
    10081 cagtctgtca accctgtcac gtcctgccac ctgtttgaca gcgggtggca ggttgttaaa
    10141 cctgttagtg aggccgtaac tatggttaat gccaatcctt gctcacgtcc agaattcatc
    10201 tggcgtttcc actccctgca aaaacactgc gatcacttcg caattgccgc aaccgaaaaa
    10261 gaagcccgtt ctctgatccc tgatcagcat ggtgttctta ctggtcgttt ctctacttcc
    10321 ccacaaccaa acactaatcc ctggtatcgc aatattacca atcccggcat tgcggaggca
    10381 cactgatgga tatctgcatt cttgaaaagc catcaatgac cagcattgag atcgcggagc
    10441 tggtgggaag tcgtccagat aacgtaaaaa tatcaataga gcgcctggct aaatctggag
    10501 ttattcaact tcctgcattg caggttttcg aaaaaatcaa taacttagga ctacgccgta
    10561 gtgtcgaggc ttacgtcttc gaaggcgagc aaggcaaacg agacagcatt attgtcgttg
    10621 cgcagctctc tcccgaattc accgcccggc tggtggaccg ctggcgcgaa ctggaagaac
    10681 aagcacgcca gccgttgacc gaaatagaga tgatcgccgc aatggctgca aatgcagttc
    10741 agcagcaaaa gcggcttcat gttgttgagt caaaggttag ccaggtggta gaaaccgtcg
    10801 agcaaatcaa gaagggcaac atgccagatg gttacattgg ataccgccag ctggcggcaa
    10861 aatgcggcct gaccgaagcc aaatgccgca accttgtcaa cgcttaccgg attcccaccg
    10921 atacacatga gtttttaact ccagaggggg tattgtcgcg tcgttccatc gtggccttat
    10981 ctcccttcat gaatgctttt aaccggatga tgtcggaggc tgaacaccga ggaaaacgct
    11041 ggtatcaccc caaaatgggg caattccagg tgatcggttg gggaggtgag tgatggacat
    11101 catgcaggcg gtaaaaaaaa tcagggaagg acaggctgac gtgtgccgcc gtaaagcatg
    11161 gagtgttggt gtatgggcct ggcctgataa ttactctact accggatggt tttgtattaa
    11221 tggactccag atccgggaag ttgatttatc gacggctgat atagtcggta atgattggat
    11281 cgtgacttga agcgtgatgc cccggcctgg ccggggcttt tttcatgcct cattagttga
    11341 ggttgctgcg tctccgactg gtaacagagc ggtattgcgt ggttcgccag caggccagcg
    11401 atagccagac actcttgatg ttgggaatgc gcgaatatta accgcatccc cctggttccc
    11461 acccaggaca agcagatcgc cattggcgcg gcgtccgaca acgaagccta cgtgcccacc
    11521 gcctacgcgg gtaaatacaa cgatacatcc gtatgccggt gtatctaatt tctcgcccca
    11581 atccagatag gattttgccg actcaaatct tgttgagcgg atgcctacac gttccagcat
    11641 tgcaccgaca aatgctgcgc accacggcgt ttcatcgtct ttaattccgc cgcgcttgat
    11701 gtcgcgccag aactgaacga tttcagggtt gtgtttagcg ccttttattt cagtcaggcc
    11761 aatgtgctta cgggcttcaa ttagccagcg aggttcaatg tttgccatat ttttctcccg
    11821 gtcatgggtt aataagtagg caaactatgg ggagtatgtg attttgacgg tagtatcgtc
    11881 cccctgaaaa taacattctc cataccctct caaccgtacc gcatagcgga attttttacg
    11941 gtttagaggg ggaaatcctt tggacaaact acttcattca atcagtgaag cctccagctt
    12001 aggcgaactg attgaaattg ttagtcggtt gcataaatta cgtgctgttg cgacctttgg
    12061 cgtcaggaaa gccgatggcg tcagtattca gaaaacacgc cgggcagcaa ataatgccgc
    12121 agtaaatctt cttaattccc ttccgcccgg cttcgatggg gcgaaattaa ctgacgaaca
    12181 acgtcagatt cttgccgggt ataccggtga aggcggcctg acagatggtg agggcagcca
    12241 atacgaatac tacacgccac agtttatggc tgaaggtata tgggatctgt ttgctgacta
    12301 cggtattgat ggcgggcacg tactggaacc atcagcgggc acaggtattt tccaggagac
    12361 aaaacggcag ggcatgataa tgaccagcgc cgaactgtct ccgatttccg gtcgaataaa
    12421 ccagcttttg cacccggaag acgatgtgaa tatcggggcg tttgaggcgc tggcggcgaa
    12481 agacgcaatg tatgatcacg ctgttggtaa cgtaccgttt ggtgaagggc gttccggtgt
    12541 cgccgggctt gatccggcat atgcaaacga gaagaacgtt ggcaattact ttgtgctgcg
    12601 cacgatcgac aaggttaagc ctggtgggct tatcgtattg gtggtgccga atggcatgac
    12661 cgatggtgcg aaatataaaa aattgcgcga taaagtcagc cgtaaagcgg aatttttggg
    12721 cgcacatcgt atgccttctg gtacgttcag cgaatccggt acagataccg tggtggacgt
    12781 atgggtattg cgtaaacacc cggaaacctt cctggagatg atccccgaca cggatgattc
    12841 aacactcaaa tcggcaaacg tcctttggga taccttcctc aaaggaaaat ggtttacaac
    12901 cgaagggaag cgatttgttt acggcgacat ggaacgtacc agcttccgca ataccctggt
    12961 ggtgaaaaaa gacggtcgtg tatccaatga gtcgatgaaa accgcgctat ctcgtcgctt
    13021 tgatagtcgc atcaattggg atttgctcgg cgtaactact caggcatggc agggcgcgaa
    13081 agtgggcgat aaacgccttg taggcggtat ctggcatgag tttgatggtc tgaaatgggt
    13141 taaggacacg acaaccaaat cctccgcgct tgatgtaagt cgatatggtg tttcgacgtt
    13201 tggcgatctg catcgcgttc cagtcaacaa acgggattct ggctttgtca tgggaccaga
    13261 tatcggcgat cgcgtccgac tatccatcgg ttgtcagtga tgaagtggca gcaatgattc
    13321 gctttgctgg caagcagcga gaaaaagaca gggagcgcgt tatgcgcggt gcgctgatag
    13381 gtcagttgat caacaaggca cttgatttgc gtaacctggg tgtgaacgtt gatgacaaac
    13441 tggcagatgc atcccgcctg gcggcagcag aagttgcgaa gtatggacca ccgcacgcca
    13501 ttaagctgaa tgggcttgct gaagctggcg ctaaaaactg gatgacgttc actggcaatg
    13561 tgaaacagga tggttccact tctgaccttc tggcagggcg acttgatgta acggatggtg
    13621 ctgccggtat tgactttaca cgtcctgaac aagtgatagc acatcttttc agtgatgtag
    13681 cccttaatcc tataacgctg ggagactttc gtgaggcgtt tgctggtgaa ctgccagagg
    13741 acgacgacgc ggcccttgaa tacctggcga aatttgatga tatcgctatt gatgggtatg
    13801 gctgcctgct gccgatggac cgcgccacca gcggcgatat tgcaaccaaa acagcattgc
    13861 tgatgggctg gcgtgatgcg tcaacaggtg agcagaaagc gaactttgag cgtcagttgg
    13921 cgaaaatcga agagaaacgt attttcacgc cactaaataa agtcaccgtt aatctgaatg
    13981 cccgctggct ggatcgccgc ttgataaaag agttcctggc agagcagggc tatgacgagt
    14041 ttaagtacac tgaacctgat ctgaaggtcg tcgatggcat tctggtatct ccggacgatt
    14101 acgaaggtaa ggacggggta tttaccggct atcagctacg aaccgtaaac ggcaaaaacg
    14161 ggaatgagtt caagaaggcc aacaataaag acggcttcct gaaccagtta gaaaactacc
    14221 tgaacggtgt taaaccgcgt gggcagaacg ctaacgagta cctggataaa attgctcaac
    14281 tggaagttag ttttaacgac tggttacgca ctcacccgca ggctgatcag atagcccgcg
    14341 agtataacga cgcattcaat ggtttcattc cgttcacgca ttctgacgca ccgcttggcc
    14401 tggaagggct tagtggaaag cgcattcctc ttacttatca gaatgaagaa gttcggcgct
    14461 tgtcagaaga tgggcgcggc atcatgggat ttggtaccgg gctgggtaaa accacgaccg
    14521 cgcttgcgct tgaggcgtat aacttcgaaa caggtcgcac taaacgcacg tgtattgttg
    14581 ttccgaaggc ggtttatcag aactggtatc acgaggcaca gagtttctac agtgctgatg
    14641 ccttcgccaa tatgatgttt atcggtttgg atgaagttcg tgatgacagc ggaaatattc
    14701 tgaccgcgcc ggtgcttgat gaaaatggcg aaccacgcct cgacagtaac gggcagcctc
    14761 ttaccagaaa tgttgtgaaa gagtccgcca gcgccgttat cgttcagcgc atgaacatga
    14821 tcccgtcgtc aaattggcga acggtcatca tgacaaaaga acaattcgcg tctatcccgc
    14881 tacgagaaaa aaccatcgag gagaactcac agcaagctgt ctttaatgct gtggaaatgg
    14941 ggcgtcttga cctggcgtcc ggtaaacacc gtgatgcgca gaagaagaac aagattaagg
    15001 atcaggccgc gaataccggt acaacgaaga agcagaatat cccgtacttt gaggatatga
    15061 acttcgatag cgtgattgcc gacgaggggc ataactaccg caactccttt aatgccgggc
    15121 gtgaagcggg acaactggca tatttgccta acccggcagt atccaagatg gcccgcgaca
    15181 tggctgtaaa agctgcgtac atgatgaaac gcaacaacgg gcgtggtgta gtcatgctga
    15241 cggcaactcc gctggtgaac agtccgattg atgccttcaa catgctgtct actgtcattc
    15301 cgcaggaaga gtggatgcgc atgggcatca ttacgcctga tgatttcgtt cgtgtgtttg
    15361 gcaaaacagc cacagttcag gtccagaaaa tttccgggga agtggaagag aagcaggggc
    15421 tggtgggctt ccagaacctt gacggcctgc gtggtatttt ccatcgttgg accactctga
    15481 agactgctgc agatgtcgga gcaacggtta aaattcccga catcattgag aacaccttgc
    15541 agatcccgat gacaggggag caggagactg cttatgaaga gcttcgtaag cgtgctcagg
    15601 agttaagtcg caatgatgag ctgacagtcg atgaaaacgg gcatatcact aacgacaaac
    15661 cggatgattt catcttctcc atcatccgtg acatggacaa ggttgctata gacccggatc
    15721 tgtatgccag tgcgattaca ttccagttcc ccgttgagct tgcagaccag gtaaaagcta
    15781 ttgctgatgc cttgccaaaa gtagctggcg gtaaagtggc agatgatgtt gatgaggccg
    15841 aggaagacgg cgctggcggg ctggtcagta cgcgtaccag caaggtagtg aagaccgctt
    15901 ttagtgaaca tccgacgcat gttgaaatac gtgccagcat tgaactggaa gctgaaatac
    15961 ttaaggccat tgcggctgcc ggtattgata tgcagcaggt atcgcacccc atcccgccga
    16021 agtatgccgc gctgatcgaa aatcttcgtg aaggtctgaa gaacggtaag cagatcgtct
    16081 ttattgatga aaaagcccag caccagaagc tgcgccgtat tatcgccagt gcactgcaaa
    16141 tgccggagca ggagattggc attatcaatg caacgacagt tagccaggct ggcggcatca
    16201 agctgaagaa ggtgaataag ccgaccgaac ctactccaaa caaaaacggt gaatacaaag
    16261 aaggggcatg ggaaacctac tacagcaaac ttgcccaata tgaggattat ctctcagcta
    16321 aaaatgatgc tggccttgag ggcatggaag gtattgccgc cgactataac gaaggtcgca
    16381 cgcgtatcat aatctgtaac aagaaggctg aagtaggtat taacctgcac atcgggacaa
    16441 ccgatatcca ccacctgaca ctaccctgga caccagccag tatcgaccaa cgaaatgggc
    16501 gcggtgcgcg cgtaggttca ccgcaagaga aggtcaatgt tcactactat tgtggcaaag
    16561 gcacattcga cgacttccgt ctggatacgc tgaaacgtaa gaaagattgg atcaagatgg
    16621 tgatgacctc cgacatgtca gagatcgcca atggtgatgc ggatgatgct gatgaacgcg
    16681 ccattatgct ggcggcaaac ccggaagaac gccgcgcaat catggctcgc caggcgcaag
    16741 agcgtgaaga acgtctgaaa ctgaaagcac agcgcgaggc caacaatgct ctcgatatct
    16801 acctgaaagc ggcaaacgcc gccggaaaag atatcggcat gatggagaca gaactgaaaa
    16861 gcgcgatgga acagatagag tattaccagc gcaatcttga cgacctgata aaagcgggta
    16921 cgaacaggaa tgggcaaaaa tacgcgcttg agcagttgag tagctacaga aaacgtgcgc
    16981 gggtgctgcg gtttgcgatc accagggcaa aagacgcgga taccataatg aaacgctctc
    17041 gtggtgatgt ggagcgcgct attaagtctg gtgtgctgga tatcagtatc gatgttattc
    17101 agaacccaca ggaatatatg cgcactgaca agaatatcct gttgcataaa ggtagttatt
    17161 accgtgcagt cattgatgat cgcgaaacaa cagcgattgt ccgggtggaa aaaattgacg
    17221 gtgaaaaagc acaatacctc tgccgtatcg cctggctgga tagagcaagt cgttacgctc
    17281 gagcaccggg gtcaatcgtt acattgccgt ttgactctgt gattgaagcg gtaacttttg
    17341 aagagggggt agctgaatcg cgcgcaacgg ctgcgcgtgg tgttcagtgc caccagctat
    17401 caacaatact tactcgcgat cagttctatg atgctattcg caatggggta atgaatgtta
    17461 ttgctggtaa acctggtaat gcatggggcg gtgggtcagc tattaaatac tgggcttatc
    17521 gcacagatga tggtcagatc gcgctgtcat cgacctatga tggcatgata ggacagaaaa
    17581 tcgggcgtac atcggatatt gcaccagagc agtggattta ccctgatatc aacaatgagg
    17641 cgctgaagcg tgaagtggca aaaatacaag ccagtgataa tccgctgaat atcgtggacg
    17701 gagagggatt cctccaggca ttgtatgggt atgattacat ccagggaatg caggcatggg
    17761 gcgcacaagc aacgatggat gatgttgccc aggagttcga aaactggatg gctgggcgtg
    17821 gtctgtcagg tcgttcgctt aataaagtca cttctgaaga tattcttagc ttgttccgta
    17881 aaccgtccgg agaaggtctg gaatcattct ggaggaccgt gctggcatac cgtttattcc
    17941 agggcaggat gaaagccttt agcaatatcg atgatattga acggatgttt aatcaggtca
    18001 agatcgcgaa aattaaggcg aagatcgaaa aaacacgttc tgcgctggtg gcctggcgcg
    18061 atgcattgtt tgagcaatat aaatcactca gtgatgcaga tggatgggcg gcattggaac
    18121 agctggcgaa taatggggta gatggagcga aactcgccat tgttcaggct gataacccct
    18181 tggaatcacc ggcagcacag tggattaagg ttggggctgt catcaatgca tttgatgaaa
    18241 aacacattag taaggacgat tttgctgatg ctgtttctgt taatcgcctg gtagaaatgg
    18301 ctacccgtcg cgcccgtgag ttgaaagacg gcgactatgt gaatgagttg ccggatgcgc
    18361 tcaaatcggc aacgtggcag gactatgtat cgctgaaaaa cggcggggcc actgaatcag
    18421 agatcgcgaa aaggcttgag caggctgaaa gcaaaagaga agagcaggcg gcagtggctg
    18481 aagccagagc tgatgaagcg gcgaatgatg actatatcat tgtggttaat gataagccga
    18541 ttcgcgctaa ggcgcgtgtt cgcgggcgct ggtggagcgt cagcgaagac gttggcgcgg
    18601 tttatctcat tgccgaccag ccaggttcct cgacaatacg gaacgccaaa gacgcgatta
    18661 agaagattgg cgggcgtttc tggaattttg aagcaaaccc ggttgctgat gtgaactttg
    18721 accgtcctgc atggatggta tctacgcgtt actcggtaga tgaactgcgt aaaataatcg
    18781 ccgatgcggc ctaacagata aggagcggcc ccaaccgggg ccgaatttga atgacgacat
    18841 taattgacac tgtaaaaccg acggaatcct accttgagga cattttgccg caggcgctca
    18901 atggcaggag tgaagaagaa tacctgacag tttatcttga tggcatggta gaaagactga
    18961 agaaaaatcc acagctttat cacctttatg gtccgtggtg gcccgcgctc aaaacgatat
    19021 tgctggagcg cggcgacata tcatttggac aagttgtaga tagtgatgtg gcagagatct
    19081 acaaaatgag tcgcccggcg cttacggttt tggctgggca tctgtattcg agcgatcgcc
    19141 tggaaaatga cgcggtatac aatcccgttc atgctctgga agtcgcacct tatgctgatg
    19201 atacagagcc atacgtgtac ataagttacg acgagtctat agagaaatat cgaatcatgg
    19261 ggtagccatg cgaaagaatc gtaggtttac ggtcgaagac ctaaaggaat attccatatc
    19321 gaaggggtat gtccttgagt tccaccgtta caagaaggtt tttacgctgc gaaaagcaga
    19381 aaaccctgcg aactggagct ggatatactt tcctcacacc gatgataagt tggtagaact
    19441 tgttgatgat ttaacctatg aaggttggtt gatcgcaatc gacaagacaa tcaaagaatt
    19501 atccgaacag gataaaataa ccctttaaaa ataacaggtt gggtaaatgc catgcgattt
    19561 accctccttg ttgattaagg tcactcttta ctctaaaagc aaatccgacc tgttgcatat
    19621 agaccatcct tgagtagtat ctctcttgtt ttacaaatct aacgttgttg ttattatttc
    19681 gcatatttac catcaatggg tgtgttagaa aaatagcgcg aaagagaagg cgtcgcagtg
    19741 aatattctga aacatcccca catgccgcag ctcttagaga actggagaaa aacccagatc
    19801 taatcaaaag gaaccatgag cattacgagc aggttcgcgt cctggactgg ttgtatcgca
    19861 atatgcgtta tgtgtatgag catactcacg ccactccaaa cggtggcctg cgaggcataa
    19921 ggacggcaat aaaaatggtg gcagagggcc aaaagaaggg atatcccgat ctttctatag
    19981 accttgcttg tgggggatac cacggaatgc gcattgagat gaaacatggg agaaatcgcc
    20041 tgactcccga acaactcgtc tggatgaccc gcctcactga agccggttac tactgctttg
    20101 aggcgcgcag tgccgctgaa gccataaaag ctatcacgga gtatgtttgt cttgattaaa
    20161 cggagaaatc gttttgcgtt tacttaacgt ggcagaactt atccctgcag gaactaatgc
    20221 tatcgctcgt cgtcgctcat ggcagcaggc tctatgtatt gagatctcga aacgtatcaa
    20281 tggaagtttt gaagctcgca atattgttac tggcgataag gttcacataa ccccggaaag
    20341 tgcaagcgct gatgattggg agtttgtaca ttgagcaaaa aatattatgt gagtctggcg
    20401 ttcgctgacg atgcggggag gacgcggagt atcacgttga gcacaccggt taaggctgtg
    20461 acagcgccgt taatccgtga agctctcaga gagctggaat tgggggaaaa ctcggcactg
    20521 ttgtcggtta gttggttagg gaaaatgtca gagaaacaat acgtggatgg agttacccca
    20581 ataacagtaa tgaggctact ttcattgctg caatgggcca tcgtacctgt atttatcgcg
    20641 tatcttattt atcaggctgc aacacagtaa ttgcataaaa acccgccaat tagcgggttt
    20701 tttgtttatt gcat
//
LOCUS       DAZFWP020000085        20615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFWP020000085 DAZFWP020000000
VERSION     DAZFWP020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.085"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            265..591
                     /locus_tag="ACHK0Z_RS14855"
                     /old_locus_tag="pgaptmp_002971"
     CDS             265..591
                     /locus_tag="ACHK0Z_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HGU4502955.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            595..1071
                     /locus_tag="ACHK0Z_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     CDS             595..1071
                     /locus_tag="ACHK0Z_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4502956.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPRSQDSVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            1068..1505
                     /locus_tag="ACHK0Z_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             1068..1505
                     /locus_tag="ACHK0Z_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4502957.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKAASII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGINRITAQVNYLQEYIRTQCLK"
     gene            1935..2186
                     /locus_tag="ACHK0Z_RS14870"
                     /old_locus_tag="pgaptmp_002974"
     CDS             1935..2186
                     /locus_tag="ACHK0Z_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502958.1"
                     /translation="MSSTKLVKTILFHGDNKYEVYATSEYADVDRIIHYKLRSCKNTD
                     GSDGKSESIWVVYNPAVQLPDDLNYKKVSNIAPYLEKLQ"
     gene            2399..2749
                     /locus_tag="ACHK0Z_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             2399..2749
                     /locus_tag="ACHK0Z_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4502959.1"
                     /translation="MPPRIPKACRARGCRNTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDSIRARVLKRDKGLCQLCLRSGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            2875..3369
                     /locus_tag="ACHK0Z_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             2875..3369
                     /locus_tag="ACHK0Z_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGU4502960.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEENLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            3366..5099
                     /locus_tag="ACHK0Z_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             3366..5099
                     /locus_tag="ACHK0Z_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4502961.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVVCCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNINRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            5111..5293
                     /gene="ymfR"
                     /locus_tag="ACHK0Z_RS14890"
                     /old_locus_tag="pgaptmp_002978"
     CDS             5111..5293
                     /gene="ymfR"
                     /locus_tag="ACHK0Z_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HGU4502962.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQLSVGGGK"
     gene            5293..6534
                     /locus_tag="ACHK0Z_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             5293..6534
                     /locus_tag="ACHK0Z_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4502963.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVEPKLNSSWEPIYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            6512..7162
                     /locus_tag="ACHK0Z_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             6512..7162
                     /locus_tag="ACHK0Z_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4502964.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            7177..8382
                     /locus_tag="ACHK0Z_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             7177..8382
                     /locus_tag="ACHK0Z_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4502965.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            8432..8632
                     /locus_tag="ACHK0Z_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             8432..8632
                     /locus_tag="ACHK0Z_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502966.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            8635..8958
                     /locus_tag="ACHK0Z_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             8635..8958
                     /locus_tag="ACHK0Z_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4502967.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTDVEKMELPMSFNWLVGPY
                     RYFPQ"
     gene            8955..9365
                     /locus_tag="ACHK0Z_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             8955..9365
                     /locus_tag="ACHK0Z_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4502968.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGNNDDFLFAR"
     gene            9340..9846
                     /locus_tag="ACHK0Z_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             9340..9846
                     /locus_tag="ACHK0Z_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502969.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRGHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            9843..10403
                     /locus_tag="ACHK0Z_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             9843..10403
                     /locus_tag="ACHK0Z_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502970.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            10412..10582
                     /locus_tag="ACHK0Z_RS14935"
                     /old_locus_tag="pgaptmp_002987"
     CDS             10412..10582
                     /locus_tag="ACHK0Z_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGU4502971.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDDKL"
     gene            10566..12062
                     /locus_tag="ACHK0Z_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             10566..12062
                     /locus_tag="ACHK0Z_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4502972.1"
                     /translation="MTISFNTIPSNTLVPIFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKDTQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLSAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            12062..12418
                     /locus_tag="ACHK0Z_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             12062..12418
                     /locus_tag="ACHK0Z_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4502973.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRMVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVGKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            12418..12687
                     /locus_tag="ACHK0Z_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             12418..12687
                     /locus_tag="ACHK0Z_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4502974.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            12829..14664
                     /locus_tag="ACHK0Z_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             12829..14664
                     /locus_tag="ACHK0Z_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4502975.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKRFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRLMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFETGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDPAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            14725..16053
                     /locus_tag="ACHK0Z_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             14725..16053
                     /locus_tag="ACHK0Z_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HGU4502976.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNAGTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVASI
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            16050..17129
                     /locus_tag="ACHK0Z_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             16050..17129
                     /locus_tag="ACHK0Z_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4502977.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTQTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            17129..17677
                     /locus_tag="ACHK0Z_RS14970"
                     /old_locus_tag="pgaptmp_002994"
     CDS             17129..17677
                     /locus_tag="ACHK0Z_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4502978.1"
                     /translation="MRTIETMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPRARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            17677..18102
                     /locus_tag="ACHK0Z_RS14975"
                     /old_locus_tag="pgaptmp_002995"
     CDS             17677..18102
                     /locus_tag="ACHK0Z_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4502979.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            18089..19147
                     /locus_tag="ACHK0Z_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             18089..19147
                     /locus_tag="ACHK0Z_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4502980.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPITCSTTGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            19138..19722
                     /gene="ymfQ"
                     /locus_tag="ACHK0Z_RS14985"
                     /old_locus_tag="pgaptmp_002997"
     CDS             19138..19722
                     /gene="ymfQ"
                     /locus_tag="ACHK0Z_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HGU4502981.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLSGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            19726..20376
                     /locus_tag="ACHK0Z_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             19726..20376
                     /locus_tag="ACHK0Z_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4502982.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRDVGTGENQIPDMSAWKRNPSSNRWRKLPDGTIIQMGISASGPLGSPVNITLPISF
                     SNTNYCVVASYDNARSGVSTMVSFAALPVSPSQFSLMSSVTEQGVNPFAYWIAFGD"
     gene            20380..>20615
                     /locus_tag="ACHK0Z_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             20380..>20615
                     /locus_tag="ACHK0Z_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4502983.1"
                     /translation="MDRYFYSQKENGFFTDLNKAPSDAVEITTDEWLSLLDGQDNGMK
                     IVSNQEGYPVLTEQPPLSKENLIALAELKKGKLI"
BASE COUNT         4892 a         5102 c         6058 g         4563 t
ORIGIN      
        1 ttttatgtgg ctgaaaattc tatcaaaaca ctaacgcgta cgcaaaaagt attgtattgt
       61 gttaagagtg gttacttcgc cacacagctt aaacccgccg tcgagcgggt tttgtcgttt
      121 ctgggcctgg ggattcgttg ggcctagtct atcccgcagt tatccattgg ctcggcttct
      181 ttgacgtttc cgcttctgat ttgcggtaca tgatgtttcc tcaatttgca cctgctgtat
      241 cagcgaggtg agagataact acaaatgcct cataacccaa atacttggct ggagttggtc
      301 cagagctggt ggcgtggaga cacaccgctg ggcgcagtga ttatgtcgat cgttatggct
      361 ggcttgcgca ttgcctattt tggcggtggt ggtggctgga agagaaaaac gctcgagatt
      421 ttgctctgtg gcgctctgac gctgaccttt gcatccgcgc ttgagtatgt cggatggcct
      481 aaatcgcttt ctgttgccat tggtggtggc gttgggttga tcggtgtcga tgctattcgt
      541 ggggctgcaa tgagagtaat cggtaacaag tttggtggct ctaaggagta atttatgcag
      601 gtactaaatt cccagcgtaa agctttcctg gatatggtgg catggtcaga aggaacggat
      661 aacgggcgac aaccgacacg taaccacggt tatgacgtta ttgtcggtgg cgaactcttc
      721 actgattact ccgatcaccc tcgcaaactt gtcacgctaa acccgaaact caaatcaaca
      781 gctgcaggcc gttatcaact tctttcacgt tggtgggatg cttaccgcaa gcagcttggg
      841 ctgaaagact tctctcccag aagccaggac tcagtggcat tacagcagat taaagagcgt
      901 ggcgctttac cgatgattga ccgcggcgat attcgtcagg caatcgaccg ttgcagcaat
      961 atctgggctt cactgccggg cgctggttat ggtcagttcg agcataaggc tgacagcctg
     1021 attgcaaaat tcaaagaagc gggcggaacg gtcagagaga ttgaggtatg agcagagtca
     1081 ccgcgattat ctccgctctg gttatctgca tcatcgtctg cctgtcatgg gctgttaatc
     1141 attaccgtga taacgccatg acctacaaag agcagcgcga taaagccgca tccatcatcg
     1201 ctgatatgca gaagcgtcaa cgtgatgtag cagaactcga cgccagatac acaaaggagc
     1261 ttgctgatgc taacgcgact atcgaaagtc tccgtgctga tgtttctgct ggtcgtaagc
     1321 gcctgcaagt cgccgccacc tgtgcaaagt caacgaccgg agccagcagc atgggcgatg
     1381 gagaaagccc aagacttaca gcagatgctg aactcaatta ttaccgtctc agaagtggaa
     1441 tcaacaggat aaccgcgcag gttaactacc tgcaggaata catcaggacg caatgcctga
     1501 aatgatcggg cgatgaaaac caaaaaaaca ggagcaatac atgactaagc tttatcaccg
     1561 catctcagct tttctctctg ggtgctgggc gtttatcacg tctatttcgt tcgtcatctt
     1621 tagtttcggt agcacagcat gctcgcttag tcggggtctg tggcgtgcta tttcagcact
     1681 agcgccgaaa tttttacctg aaaaggttgt ttggcgaatt gtagagcgaa tgtgtagtga
     1741 gagcgttcgc gagaagatta acgtatttgg acgtcatcct cgaaatacag gcgcattgtg
     1801 cagttcgtta ctgtagtcat tacaaagccc atctttgggt ttggtttgta atttcgctgc
     1861 tgcaataatc accagaaata tggcattgta attggtggtc atatgtcata cccatcaatt
     1921 acaaaggagc agacatgtca tctacaaaat tagttaaaac tatccttttc catggagata
     1981 ataaatatga agtttatgca acctccgagt acgctgacgt agatagaatc attcattaca
     2041 agttacgctc atgtaaaaac acagatggtt cagatggcaa gtctgaatct atatgggttg
     2101 tatataatcc tgctgttcaa ctaccggatg atctgaacta caaaaaagtt agcaacatag
     2161 ctccttatct tgaaaagtta caataaactt ccttttccac atgctaaaag cctcgtattc
     2221 gcggggcttt ttattgtcat tacaaaagcc actccctaca gagtggcttt gataatggtt
     2281 tataccctac acgggataac ttaactgata tcccttttaa cggataaacg gagccaacaa
     2341 tggcagagaa tgtcggcatt atggcagtta aatttggata aatcggagat tagtacatat
     2401 gccgccacga atcccgaaag cctgccgtgc tcgaggttgc cgtaatacca ccactgaccc
     2461 gtcaggctat tgcgaaagcc acaaaagcga aggctggaag caatacaaac ctggacaatc
     2521 ccgtcatcag cgcggctacg gttcgaagtg ggacagtatc cgcgcgcgtg tcctgaagcg
     2581 tgacaaaggc ctgtgtcagt tatgtctgcg ttctggtgtg gtgcgtgagg cgaaaactgt
     2641 tgaccacatc atccctaaag cgcatggcgg cactgatgct gacagtaatc tgcagagtct
     2701 gtgctggccg tgtcataagg cgaagacggc ccgtgaacgg ttaaagtgat aataattctc
     2761 aactgtctga ggggaggggc gggtcaaatc cctgtgacct gacgtcttcc ggactgcccg
     2821 ccccatcgtt tttttatacc cgcgaaaaat gaaatttaac caggagtgcc gcatatggct
     2881 ggaacggcgg ggcgttccgg gcgtcgcccc aagccaacgg cgcgcaaggc gctggccgga
     2941 aaccccggca agcgagccct gaataaagat gaacctgttt ttacgcccat caaaggtgtt
     3001 gagccaccgg agtggttcgc agaagaaaat ctccctctcg ccacgatcat gtggcaactg
     3061 acaaccaaag aactctgcgg tcagggcctg ttgtgcgtga ctgacctcgc ggtgcttgag
     3121 cggtggtgcg tggcctatga gttctggcga cgtgccgtga aaaatattgc catacagggc
     3181 aacaccatca ccggtgcaat gggcggcagg gtcaaaaatc cggagctgac cgccaaaaaa
     3241 gaacaggagt ccgagatgag cagcacgggg gcaatgctcg gactcgaccc cagcagccgc
     3301 cagcgtctga ttggcctggc ggggcagaag aaagccacta acccgtttct gaaaatcatc
     3361 gagtcatgag ccggaaatct taccccaacg taaatgctgc caatcagtat gcccgtgatg
     3421 ttgtgcgcgg aaagattgtg gcctgccagt ttgtgattca ggcctgccag cgccatcttg
     3481 atgacctgat ggcggaaaaa agtaagtcgt ttcgttaccg cttcgacaag gacctggctg
     3541 aacgggccgc caaatttatt cagctgttgc cgcacaccaa gggtgagtgg gcattcaaga
     3601 ggatgcccat cacgctggag ccgtggcagc tatttgtggt ctgctgtgcg tttggctggg
     3661 tcaataaagg gtcccggctg cgccgcttcc gggaggtgta taccgaaatc ccccgtaaga
     3721 acggcaaatc ggcaatctct gccggtgttg ccctgtattg ttttgcctgt gataacgagt
     3781 ttggcgcgga agtgtattcc ggtgccacga cagagaaaca ggcgtgggaa gtctttcgcc
     3841 cggcgcgact gatgtgtaaa cgcacaccca tgctgacgga agcgttcggg attgaggtta
     3901 acgcctcaaa cattaatcgt ccggaggatg gcgcgcggtt tgaaccgctg atcggtaacc
     3961 ccggtgatgg ttcatcaccc cactgtgcgg tggtggatga atatcacgag cacgccaccg
     4021 atgcgcttta caccacgatg cttaccggga tgggggcgcg acgtcagcca ctgatgtggg
     4081 ccattactac tgccgggtac aacattgagg ggccgtgcta cgacaagcga cgggaagtta
     4141 tcgagatgct caacgggtcg gtacccaacg atgaactgtt cgggatcatc tataccgttg
     4201 acgaaggcga tgactggacc gacccgcagg tgctggaaaa agctaacccg aatattggcg
     4261 tgtcggttta tcgcgaattt ttgttaagtc agcagcagcg tgcgaaaaat aacgcccgtc
     4321 tggcaaacgt ctttaaaaca aaacacctca atatctgggt gtcggcgcgt tcggcgtatt
     4381 tcaacctggt gagctggcag agctgcgagg ataaatcact gacccttgag cagttcgagg
     4441 ggcagccgtg cattctggcc tttgacctgg cgcgtaagct ggatatgaac agcatggcgc
     4501 gactttatac ccgcgagatt gacggtaaaa cgcattacta cagtgtggct ccgcgcttct
     4561 gggtaccgta tgacacggtg tacagcgtcg agaaaaatga agatcgccgg acagccgaac
     4621 gctttcagaa atgggtggaa atgggcgttc tgaccgttac cgatggtgcg gaggtggatt
     4681 atcgctacat cctcgaggag gccaaagcgg cgaacaaaat cagcccggtc agtgagtcac
     4741 ccatcgaccc cttcggggcg accgggttgt cacatgacct tgctgatgaa gacctgaatc
     4801 ccgtcactat cattcagaac tacaccaaca tgtccgaccc gatgaaagag ctggaagcgg
     4861 caattgaatc ggggcgcttt catcatgatg gcaatcccat catgacctgg tgtatcggca
     4921 atgtggtcgg caaaaccatt ccgggtaacg atgatgtggt gaaacccgtc aaagagcagg
     4981 cggaaaacaa aattgacggt gcagttgcgc tgattatggc ggttggcaga gccatgctgt
     5041 acgagaaaga agacacgctg tctgaccaca ttgagtccta tgggatccgc tcgctttaac
     5101 tgaggtaatt atgatcatgc tgattctcgc gcctctggtg ggcgtgctgg gggcgctttt
     5161 gctggcgtat ggtgcctggc tgatttatcc cccggcgggg tttgttgttg ccggggcgtt
     5221 gtgcctgttc tggtcgtggc tggtagcgcg atatctcgat cgtacacagc tgtctgttgg
     5281 tggaggtaaa tagtgttctt ttcgggatta tttcaacgaa aaagtgacgc accggtgacc
     5341 acgccagcag agctggcgga tgccatcggg ttgtcctacg acacctatac cggaaagcag
     5401 atcagcagtc agcgggccat gcgactgacg gcggtttttt cctgcgtcag agtgctggca
     5461 gagtcggtcg ggatgttgcc ctgcaatctg tatcacctga acggcagcct gaagcagaga
     5521 gccaccggcg aacgtctgca taaactgatc tccacgcatc ccaatggcta tatgacgccg
     5581 caggagttct gggagctggt ggtcacctgt ctgtgcctgc ggggaaactt ttacgcctac
     5641 aaagtgaaag catttggcga agtggctgaa ctgctgcccg tcgatcccgg ctgtgtggaa
     5701 ccgaagctta acagtagctg ggagccgatc tatcaggtca cattcccgga tggctccacg
     5761 gatgtactga gccaggagga tatctggcat gtgcgcacgc tgacgctgga cggactggtg
     5821 gggctgaatc ccatcgccta tgcccgcgag gcaatatcgc tggcggcagc gaccgaagag
     5881 cacggggcca gactgttcag caatggcgcg gtgacgtcgg gtgtgttgcg tacagagcag
     5941 acgctgtcag atcaggctta tgagcgcctg aagaaagatt ttgaggagcg tcacaccggg
     6001 cttggcaatg ctcaccgccc gatgatcctt gagatggggc tggactggaa gtcgatggcg
     6061 ctgaacgccg aggacagcca gttcctggaa acccgcaagt ttcagcttga agaaatctgt
     6121 cgtctgttcc gggtgccgtt gcacatggtg cagaacaccg atcgcgccac cttcaacaat
     6181 atcgaagagc tggggctggg atttatcaac tattcactgg tgccgtatct gacccgcatc
     6241 gaacagcgga tcaacaccgg actggtacga aaaagtaagc agggcgttta ttacgccaaa
     6301 tttaacgccg gggcgttact gcgcggggat atgaagtccc gttttgaagc ctacgccacc
     6361 gggatcaact ggggaattta ctctcccaat gactgccgcg acctggaaga tatgaatccg
     6421 cgtcccggtg gtgatgtcta tctcacaccg atgaacatga ccacgaaacc ctccgatggc
     6481 agtaaagccg gtaagcagaa ggataacgcc aatgcagacg aaacaacgtc ttgatgtacc
     6541 gctgagtctg aaatctgtca gtgactccgg tgagtttgaa gggtatggct ccgtctttgg
     6601 tgtaaaggac agccacgatg atgtggtgat gtccggggca tttgctgctt ccctgcgggc
     6661 gtggagtgac agaaaagcgt tacctgcgct gctctggcag caccgcatgg atgaacccat
     6721 cggtgtttac accgaaatga aggaagacga tgtcgggctt tacgtcaggg gacggttgct
     6781 tattgatgat gatcccctcg caaaacgcgc acatgcacac atgaaggccg gttcgttaac
     6841 cggcctttct attgggtacg tcctgaaaga ctgggaatac gaccggagca aagaagcctt
     6901 tctgctgaaa gaaatcgacc tctgggaagt cagcctggtg acgttcccgt ctaacgacga
     6961 ggcgcggatc agcgacgtca agaacgcact ggcccgcggg gaaatccccg aacagaaaaa
     7021 aatcgaaaga gtcctgcgtg atgtcggact ctcccgtacc caggccaaag cattcatggc
     7081 cgggggctat ggcgcactgt ccctgcgcga cgctgaggat gtgggctctg cactgaatgc
     7141 actgaaaaat ctgaacttct aatcaggaga aatacgatgg cggttgatat taaagatgtc
     7201 gaacaggtcg cgcaggagct gcagcagaag tttgacgact tcaaagcaaa gaacgacaag
     7261 cgcgtggatg cgattgagca ggaaaaaggc aagcttgccg ggcaggtgga aaccctgaac
     7321 gggaaactca gcgagctgga aaatctcaaa agcgaccttg aaaaagagct gcttgagctg
     7381 aaacgtccgg caggtggtgc gcaaaataaa ctggccaccg agcataaaga ggcgtttgtg
     7441 ggcttcctgc gtaaaggccg tgaagacggt ctgcgcgatc tggagcgtaa ggcattgcag
     7501 gtgggtaccg atgaagacgg tggctacgcc gtgccggaag aactggatcg caacattctt
     7561 aacctgctga aagatgaagt ggtgatgcgt caggaagcca cggtgatcac cgttggcggt
     7621 tccgactaca aaaaactggt gaatctgggc ggtacggctt ccggatgggt gggggaaacg
     7681 gatacgcgat cccagactgc cacctccaga ctggagctga ttgaacctct catgggggaa
     7741 atttacggca acccgcaggc tacccagaaa atgctggacg atgccttctt caacgtggag
     7801 gcctggatca acagcgagct ggcaaccgaa tttgccgaac aggaagaaat tgcctttacc
     7861 tcaggcgatg gcaccaagaa gccgaaaggg ttcctggcgt atgaatccac tgatgaaacc
     7921 gacaaggtcc gggcgttcgg caaacttcag catattgtat ccggcgaagc gaccgcggtg
     7981 accgcagacg ccattatcaa actgatttac acgctgcgta aggcacaccg cactggcgcg
     8041 aagttcatga tgaacaacaa cagcctgttt gccatccgtc tgctgaaaga caccgagggt
     8101 aactatctgt ggcgtcctgg gctggaactg gggcagccat cctctctggc gggttacggt
     8161 atcgctgaaa acgagcagat gccggatatt gccgcggatg cgaaagccat tgcatttggt
     8221 aacttcaaac ggggttacac catcgttgac cgtatcggta cccgcattct tcgcgatccg
     8281 tacaccaata aaccgtttgt cggtttttat accaccaagc gcaccggcgg catgctggtc
     8341 gattcgcagg ccatcaaact gctgaagatt gcagcggcgt aatcactcag gggcgcggaa
     8401 ccgcgccccc tgttctgacg ggtgaagaat catgatcctg aaacaagatc tgaaatggtc
     8461 accggacggt atgcgtgttg aggtcattcg ggccggtgag tatgacgacg gggcgcttcc
     8521 tgcccgggtg caggagattg cacttcaggc cgggttagca gagcgcggaa ccagtgcaaa
     8581 aagcagtaaa gcgacaaaag agaaaaaagc cacgaccagt aaagagggct gagtatgctt
     8641 ctgacaatgg aagagattaa agcccaactc cggctggatg aggatttcga tgctgatgac
     8701 cgccatctgc aactgctggc ctgtgcggcg caaaagcgga cggaaacgta tctgaaccgg
     8761 aagctctatg ctccggatga aaccattccg gacagcgatc cggacgggct gcacctgccg
     8821 gatgatattc gtctggggat gctgatgctt atcagccatt tttacgaaaa tcgctcgtcg
     8881 gttacagacg ttgagaaaat ggagttgcca atgagcttta actggcttgt cggcccgtac
     8941 aggtatttcc cgcaatgaaa attcgtcagg cgcagaccag cgcaacctac attctgccgg
     9001 accccggtga actgaataaa cgcgtcctga tccgccagcg ggtggatatg cccgcggata
     9061 actttggcgt ggagcctcaa tacccggtta cgttccggac atgggcgaag gttatccaga
     9121 ccagtgccac cacctggcag gaaaccgcgc agaccgggga cgccatcacc cattacatca
     9181 ccattcgtta ccgccggggg atcaccgctg attatgaggt ggtctgcggt gacagtgtgt
     9241 accgggtgaa acgtcagcgc gatctgaacg gggcgcggcg ctttctgctg ctggagtgta
     9301 cggagctggg cgaatgtagg cagagtcacg gaggcaacaa tgacgacttc ctttttgcac
     9361 gttgattttc agcagcccgc ggagatgcgc tttaaccgcg cccgtgtccg gcgggcgttt
     9421 gtcacgattg gtcagcgtca tatgcgtgat gcccgtcggc tggtgatgcg ccgtgcacgg
     9481 tcggcaccgg gtgaaaaccc cggttatcag accggacgcc tggctcgttc aattggttac
     9541 atggtaccca gagccagtaa acatcgccct ggttttatgg cacgtatagc ccctaaccag
     9601 cgtaatggag agggaaaccg ccgtatcacc ggtgattttt atccggcttt tttgttctat
     9661 ggcgtgaggc gaggggcaaa gcgtcgtcgc ggccatcatc gtggtgcatc cggtggcagc
     9721 ggctggcgac tggctccacg taataacttc atggtggaaa ctcttgaaaa gaaccgcagc
     9781 tggacacgct attttctggc gcgggaattg cgtaaatcac tgaagccgga gcgacgacac
     9841 agatgaaact gacgcctgtt attgctgcac tgcgtgcccg ttgtccgtat tttgaaaacc
     9901 gggttgcagg cgcggcccag ttcaaaaatc tgccggaggt cggaaagctg aaactcccgg
     9961 cggcatatgt tgtaccgggt gatgattctc cgggagaaaa caaaagccag accgactact
    10021 ggcaggagct gaaagagggt ttctccgtgg ttgtcatact gagtaacggg cgtgatgagc
    10081 gcggtcagtt tgcctcgtat gatgtggtgg acgatgtccg gcagatgctc tttaaggctc
    10141 tgctgggctg gaacccggag gcgtgcggta acccgattac ctatgacggc ggcacgctgc
    10201 tggatctgaa tcgtcatgag ctgatttatc agttcgattt ttcggtcatc agcgagctga
    10261 ctgaagacga tacccgccag caggatgacc tgaacagtct ggatgaactg cgaacgctgg
    10321 cgattgatgt tgattatctc gatcccggta acgggcctga cggcgatatc gaacatcaca
    10381 ccgaaataac ccttccttcc tgagaatctt catgtttgtg aaacctgtta aagggcggtc
    10441 agttcctgac cctgcccgcg gtgacctttt gcccgccgag gggcgaaatg ttgatgagaa
    10501 caactactgg ctgcgccgtg aagcagcggg tgatatccgg cgcgtgaatg aaaaggtgaa
    10561 taccgatgac gataagcttt aacaccattc cgtcgaatac gctggttccg attttttatg
    10621 cggaaatgga taaccaggcg gcgaatactg cacaggacag cggagcatcg ctgctgattg
    10681 gtcatgccaa taacggtgca gagattgttg ccaacagtct ggtgctgatg ccatcggcag
    10741 actatgcacg ccagatttgt ggtgcgggaa gtcagctggc gcgtatggtc gaggcttatc
    10801 gccagaccga cccgtttggt gaactgtatg tgattgccgt tcctgaatcc acgggcgcgg
    10861 cggcaacagt tacgctgacg gtgaccggcg cggcaaccga aaccggcacg gtgaatgttt
    10921 atgtgggacg tacccgcgtg caggctccgg tgaccaacgg cgataacgtc acgacgattg
    10981 ccagcagtat caaagatgcc atcaatgccg ttccggccct gccgtttacg gcttcatctt
    11041 cggcaggcgt ggtcacactg accgcgcgtc ataaggggct ttgcgggaat gaaattcctg
    11101 tcagcctcaa ttactacggc tttggtgggg gcgaagtgct gccagcgggc gtacagattg
    11161 ccgtggcgac gggtaccgcc ggtacgggtg ctccggttct caccggcgcg gtggctgcaa
    11221 tggcggatga gccgtttgat tatatcggcc tgccgttcaa cgacacggcc tccgttaaca
    11281 cgctggtgac cgagatgaac gataccagcg gtcgctggag ctatgcgcgt cagctgtatg
    11341 gtcatgtgta tacggcaaag atcggcacgc tgtcagaact ggtgaccgca ggtgaccagt
    11401 ttaaccagca gcacattacc ctggcggggt acgaaaaaga cacccagacg cctgccgacg
    11461 agctggcggc aagccgtacc gcccgcgcag cggtgtttat ccgcaacgat ccggcacgtc
    11521 ccacgcagac cggtgagctg gtgggtatgc tgtctgcgcc gaaggggaaa cggttcacga
    11581 tgaccgaaca acagaccctg ctgtctcatg gcgtggcaac ggcgtatgtc gaaagcgggg
    11641 tactgcgcat tcagcgtgat gtcaccacgt acaggaaaaa cgcttacggg gttgcggata
    11701 acagctacct cgacagcgag acgctgcata ccagtgcgta tgtactgcgc aaactgaaat
    11761 ccgtcattac cagtaagtac gggcgtcaca agcttgccag cgacggtacc cgctttggtc
    11821 ccggtcaggc gattgtcacc ccggcggtga tcaaagggga actgctggca acctaccgtc
    11881 agcttgagcg tgcggggatc gtggaaaact acgaactttt taagcagtac ctggttgtgg
    11941 agcgtgatgc cagcgatccg aaccgcctga acacgctgtt cccgcctgac tatgttaacc
    12001 agttgcgtgt ctttgccgtg gttaaccagt tccgtcttca gtattcagag gagtctgcat
    12061 aatggcccgt atcgggggaa cctgttattt caaaattgac ggtcagcagc tatcgctgac
    12121 cggcggcatt gaggtgccca tgaacaggat ggttaatgat gacatcatcg gcctggacgg
    12181 ttcagtggac cgcaaggaaa ctcaccgtgc gccttatgtc aaagggacct tcaaggtgcc
    12241 gaagaatttt ccggtgggca aaatcacctc gtctgatgag atgacaatca ctgccgagct
    12301 ggcgaacggt caggtctatg tactgtcgtc tgcttggctg cacggcgaag cgaaccataa
    12361 tgccgaagaa ggcacggtcg atcttgagtt ccacggtgaa gaaggggatt accagtaatg
    12421 aaagagcttg agttaaagaa accgattacc gctcatggcg agacactctc cgtactggag
    12481 tttgatgagc ccaccgggaa agatgtccgc gagctggggt atccctacca gatgaatcag
    12541 gatgagtccg tcagacttct ggcgcatgtg gtatcgaaat atattgtgcg gctggcgaaa
    12601 gtgccgcaaa gctctgtcga ccagatgtct ccggcagacc tgaatgcagc ggcgtggctt
    12661 gtggctggtt ttttcctcca ggcctgacgg ctgaatacct tactgatcgc ttctttgact
    12721 gcgccagcta ctggcgcatt aatcctttcg aattgctgaa tatgccgatc agtgaaattc
    12781 ccttgctggt cagtcaggca aacaggatag agcaggagaa acgcacacat ggctgaattt
    12841 gagcttaagg cgttgatcac cggtgtcgac aggctttctc ccgcgctgtc gaaaatgcaa
    12901 aagaaaatac ggggatttaa acgccaggcg gaagaagcgt cacagggtgg gctggcgctt
    12961 ggtggcggac tggcagcggg tctgacgctt tccctgaaat cttatgccga tcaggaaaac
    13021 gccgccaccg ggctgaaagt cgccatgatg gatgcgaatg gcgaggttgg aaagcgcttt
    13081 caggacatca ataaactggc tattggcctg ggtaaccagc ttcccggtac aacggctgat
    13141 ttccagaaca tgatgcagat gctggtgcgt caggggatcc cggcagaaaa cattcttggc
    13201 ggtgtgggta aagcgacagc ttatcttgcg gtacaactga aaaaaacacc ggaagcggct
    13261 gctgagtttg ctgcaaagat gcaggatgct accggaacgg cgtcagaaga catgatgggg
    13321 ctgttcgaca ctatccagaa ggcgttttat ctgggcgttg acgataccaa catgttgtcc
    13381 ttcttcacta aaaccagttc tgttctgaag atggtgaaca aggacggtct tcaggctgca
    13441 cagagccttg cccccatcag cgtcatgatg gatcagatgg ggatgaacgg ggagtcggca
    13501 ggtaatgccc tgcgaaaagt tatccagtcc ggattaagcg ttaagaaaat cagggacgtc
    13561 aataaaatca tggcccgcca gaaactcggg gtacagctcg attttactga cggcaaagga
    13621 agttttggcg gtcttgataa catgttcagg caactggcaa agctgcgaaa actgaccgac
    13681 gttaagcgga caggtgtact taaggcaata tttggtgatg atgccgaaac ccttcaggtg
    13741 gtcaatgcac taatcgataa aggaaaggat ggctacgatc agatccagca gaagatgaat
    13801 aaacaggcca gcctgaataa acgtgttcag gcccagcttg gtacgctgtc caacctgtgg
    13861 gaggcaatga cggggaccgc aactaacggc cttgcggcta ttggcggcgc attttctggt
    13921 gacgccaaaa atatcacgca atggctgggg gagttggggg aaaaattcac gaagtttgcg
    13981 gatgaaaatc cccgggttat tcgcggcgtc gtcgggcttg ctgccggtct tgcgattctg
    14041 aaactgggat tgatgggcgt tggcagtgcc atcagtattg tcagcaggct catgtcgatg
    14101 acgccgattg gcatgattgc gacggcgata gccctggctg cgggattaat tatcactaac
    14161 tgggatgttg tcggacctta ttttaagaaa ctctgggaaa ccattggtcc ttattttgag
    14221 actggctggg aactccttaa gaaagttttt gcctggtcgc cgctggggat ggtgatcaat
    14281 aactgggggc cggttgttaa gtggtttcag gatatgtggg acaagctgaa gccaattatt
    14341 gagtggttta ccgacagttc cggtgacacg gtcgatgcca ttaactcggc gcagtggggg
    14401 gcgggtgctt atgatgctta tgggacggga ataccggcac ggggatacac accttatccg
    14461 gcggtagatc cggctcagtc aaacaacgcc tccgatgcca caggcccgaa tcccttcatg
    14521 attaacaaag cttctgcgcc aaaagttgat ggtgagatca aggtctcttt tgtgaattcg
    14581 cctccgggta tgcgggttat ggaaacgcga tccagcggtt ttgatgtcag ccatgatgtt
    14641 ggctatacgc gctttggcag gtaatgaaaa attaatctgt taatgagtcc cactccggtg
    14701 ggatttttta tgtacggagt ttatatgacg tggaaagaca gacttcagga cgcgtcattt
    14761 cgcggtgtgc cgtttaaggt tgaagaagaa agtgcgggaa ccggtcgtcg tgtggaaacg
    14821 cacgaatacc cgaaccgcga caaaccctat accgaagacc tggggaaaat cactttccgc
    14881 ccgtccatca cagcttatgt ggtgggagat gactgctttg accagcgcga tcgcctgatt
    14941 gacgcgctga ataaacccgg tcccggcacg cttgtccatc cgacttacgg tgagctgaaa
    15001 gtctgtgttg acggggaagt tcgggtcagc acatcgaaga gtgaagggcg tattgtccgc
    15061 tttgacctga agtttgtcga agcgggagaa ctctcttacc ccacttcagg tgcggcgacg
    15121 gcgcagacgc tgatgtcatc ctgttctgca ctggatgact gcatcagtga cagcttcagt
    15181 ggtttcagta tcgatggcgt ggcagatttt gtgcagaacg acgtcgtcgg taatgccggc
    15241 acaatgctgg ggtatgtttc tgatgcgatg aaagtggtgg attctgccgt atcggatgcc
    15301 gccaggctgt tgcaggggga tatctcggta cttctgccgc cgccatcgtc aggcaaaaat
    15361 ttcgttgagc aggtgcagaa aatgtggcgt accgggaaac gcctttatgg taacgccagc
    15421 gacctggtca ccatgatcaa aacgctttcc ggtgtcagcc tcggcagcga tctgcaaccg
    15481 cgcggtgtct ggaaaacgga cagtaaaacc accgccacgg cgacgcagca gcgtaacgtg
    15541 gttgccagca tccttcgtac gaccgcaatc agcgaagcgg cgtatgccgt cacccgattg
    15601 cctgcgccaa caacttccgc ggtgatgcag aatgccgcag tggggcaggc aacaacaccc
    15661 gcgcagagca ctggctggcc ttccgtcacg catccggcac tgaacaatgc accggcggtg
    15721 aaaaacacgg ttgacctgcc gacgtgggaa gaactgactg acattcgcga cacactgaat
    15781 acggcaattg ataaggagtt gtcccgtaca accagtgatg cgctgtttct ggcgctgcgc
    15841 cgggtgaaag cagatctgaa tgcggatatc aacacgcgcc ttgaacagtc tgcacggatc
    15901 attcagcgca cgccggatga ggttttaccc gcgctggtgc tggcggcgac ctggtttgat
    15961 aacgcggcgc gtgacgcgga cattatccgg cgtaatgcca ttacacatcc cggctttgtg
    16021 ccggtgatcc ctctgaaggt gccagtgcaa tgaacgacaa tgtcacgcta cgggtaaatg
    16081 gccgggagtg gaatggctgg acatcggtgc gcatcggtgc cggtattgaa cggctggcgc
    16141 gggatttcag tgtggagatc actcgccagt ggccgggaga tgagggtatc accacgcttc
    16201 agccgcgcat taaaaacggt tcaaaagtgg aagtgctgat tggtgatgag ctggtgatca
    16261 ccggctgggt ggaggcgact cccgttcgtt acgatgcccg ttcggtcagc accggtattg
    16321 ccggacgtag tctgacggct gacctgattg actgtgcagc cgaaccgaca cagtttaacg
    16381 gacgctcgct ggtgcagatt gcgcaggcgc ttgctgcgcc tttcggcatt gaggtggtga
    16441 acagcggtgc gccgtcgggt gttattcctg atgttcagcc tgatcacggt gaaacggtga
    16501 ttgaggtgat caacaaaata ctcggtcagc agcaggcgct ggcttacgac gacccgcacg
    16561 gcaggctggt gattggcggt attggctcaa cgcgggcaca taccgcgctg gtacttgggg
    16621 aaaacatcct ttcctgtgat acggagaaga gtatccggga gcggttttct gtttaccagg
    16681 tggcggggca gcgtgccgga aacgacgatg atttcggtga ggccaccacc accgcgctgc
    16741 gggcacgcac agaggacgca tttattgccc gttaccgtcc gatgtatatc aggcagacag
    16801 ggcaggccac gggggcaggt tgtattgcgc gtgctgactt tgaagcccga caacgggcgg
    16861 cgcggacgga tgaaaccacc tatgtggtgc agggctggcg acagggtaac ggtacgctgt
    16921 ggcagcccaa ccagcgggtg attgtcttcg atccggtctg tggtttcgac aataccgaac
    16981 tgcttgtctc ggaagtcacg tttactcagg accagaacgg cacccagacg gaaatccgtg
    17041 tcggcccacc tgatgcttat ctgcctgaac ccgaagcccc cggcgcgcgg aaaaagaaaa
    17101 aagccagagt acaggaggac ccgttctgat gaggacgatt gaaaccatgc agcgacaact
    17161 cctcggcctg attgggcggg ccgtggtgaa aagcatcagt gccgccacga aatgtcagac
    17221 cgtggatgtg tccctgattg ccggtgaacc caaagccggg gttgaacatc ttgaacccta
    17281 cggttttacc gcaagggcaa acagcggtgc ggaagcggtg gtgttgtttc cggatggcga
    17341 ccgttctcat gcggtggttg ttacggtgtc ggaccggcgc taccgcctga aagggctgca
    17401 gacgggtgag gtggctgtct atgacgatca ggggcagtcc gtgacgctga cccgggaggg
    17461 gatcgtggtg gacggtgcag gtaaaacgat cacgtttcgc aatgcgccca gagcacgttt
    17521 tgaaatggac ctggaagtga ccggacaggt gaaagacctg tgcgactccg gcggcaccac
    17581 catgtcagcg atgcggcttg cctataacgg gcatcgtcac agagagaacg gtcagggcag
    17641 taacaccgac aaacctgata aagcgatgga ggcatgatgg aactgtggct gacggtgaac
    17701 ggtaaacgca cctgcgccag cgcaccgctg gatccgctga cccgcgccgt ggtgatttcc
    17761 ctgtttacct ggcggcgggc ggagcctgat gacaacgccg acgtcccgat gggatggtgg
    17821 ggggatacct ggcctgcggt acagaatgac cgttacggct cccgactgtg gctgcttcag
    17881 cgcagcaaac tgaccaatca gctggtgcag acggtaaggg ggtatatccg cgaatgcctg
    17941 caatggatga ctgatgacgg cgtggtgtcc cgtattgatc tggatatccg ccgcaccggg
    18001 attaatgaac tgggtaacag tatcactctc tggcgtcgtg acggaccggt aatgatttct
    18061 tttgatgatc tgtggagtgc gataacgcat ggcggacagt gaatttcagc gcccgacgct
    18121 ggcagaaaat atcagtatgc tccgtaacga tttatttgcc aggctggacg tcagcgacac
    18181 gctccggcgc atggatgaag acgtgcgggc aaaggtgtat gcggcggcgc tgcatacggt
    18241 ttacgggtac atcgattatc tggcaatgaa tatgctgcct gacctgtgcg atgagtcctg
    18301 gctggcgcga catgctgcga tgaaacggtg tccgcgcaag ggggccacgg ctgccagcgg
    18361 gtatatgcgc tgggaaggtg tcagcgatgg cctgaaggtg actgccggga gtgttattca
    18421 gcgcgatgac ctggttcagt acacggcaac tgccgatgca accagctccg gtggtgtcct
    18481 gcgtgtgccg atcacttgct caactacagg cgcggtcggt aacgctgacg acggtacggc
    18541 attaatcctg gtcacgccgg tgaatggtct gccgtcttcc ggtgttgcag ataccctgac
    18601 tggaggattc gatactgaag atctggaaac gtggcgcgcc cgcgtcattg agcggtatta
    18661 ctggacgccg cagggcgggg ctgacgggga ctatgtcgtc tgggctaaag aagtgcccgg
    18721 cattacccgc gcatggacat accgtcactg gatgggaacg ggaactgtcg gtgtgatgat
    18781 tgccagcagt gacctgatta atcccattcc ggaagaatca acggaaacgg cggcaagaca
    18841 acatatcggg ccactggccc cggtggcagg ctctgatttg tatgtattca ggccggtggc
    18901 gcataaagtg gattttcata tccgcgtgac gccggacaca ccggaaatac gggctgccat
    18961 caccgccgag ttgcgttcgt tcctgctgcg tgatggttat ccgcagggag aactgaaggt
    19021 gtcacgtatc agtgaagcga tttccggtgc gaacggggaa tacagccatc agttgcttgc
    19081 accggcagac aatatctcca ttgcaaaaaa tgaactggcg gtactgggga cgatttcatg
    19141 gacgtgacaa acgatgatta catccgtctg ttgtcggcac tgttgccccc cggtccggcg
    19201 tggtcagcca gcgatccggc gattgccggt gcggcaccgt cattaacccg cgttcatcag
    19261 cgtgcggatg ccctgatgcg ggagctggat ccgcgcacta ccactgaact gataaaccgc
    19321 tgggagcgtc tgtgcggtct gccggatgaa tgtattcccg cagggacaca gacccttcgc
    19381 cagcgtcagc aacggctgga tgcgaaggtt aacctgtcgg gtggcatcaa cgaggatttt
    19441 tatcttgcac agcttgctgc cttgggcaga ccagatgcca ccatcacgcg atacgacaaa
    19501 agcacgttca cctgctcatc ggcctgtact gacgctgtga acgcgcctga atggcggtat
    19561 tactggcagg tcaacatgcc agccgccacc aacaccacct ggatgacatg tggcgatccc
    19621 tgtgattccg cactgcgtat ctggggcgac accgttgtcg agtgtgtgct taacaaactc
    19681 tgcccgtcgc atacctacgt aatttttaaa tatccggagt aatccatgca tcgtatagac
    19741 acgaaaaccg cgcagaagga taagttcggc gcgggtaaga acggttttac ccgtggtaac
    19801 ccccagaccg gcacgcctgc caccgatctg gatgatgact actttgacat gttgcaggag
    19861 gagctttgca gcgttgtgga ggcgtccggt gccagcctgg agaaggggcg gcacgaccag
    19921 ctgcttaccg cgcttcgtgc gctgctgtta agccgcaaga atccgtttgg tgatatcaaa
    19981 tcggatggca cggtgaaaac ggctctcgaa aaccttggtt tgggagaagc ggcgaaaagg
    20041 gatgtaggca caggagaaaa tcagataccg gatatgtcag cctggaaaag aaatccgagt
    20101 tctaatcgct ggagaaaatt gcctgatgga accatcattc aaatgggaat atcagcatca
    20161 gggccattag gctcacctgt aaatatcacc ctgccgatat ctttcagcaa taccaattat
    20221 tgtgttgttg catcgtacga taatgcacgg tcaggtgtgt caacaatggt tagttttgca
    20281 gcattacctg tttcaccatc gcaattttcc ctgatgtcat ctgtgactga gcaaggagta
    20341 aatccttttg cttactggat tgcttttgga gattgataaa tggacagata cttctattca
    20401 caaaaagaaa atggtttttt taccgattta aataaagcac cttcagatgc tgttgagata
    20461 accacggatg aatggctgtc actactggat ggtcaggata atggcatgaa aatagtcagc
    20521 aatcaggagg gatatccggt tttgacagag caaccacctt tatcaaaaga aaaccttatt
    20581 gcattggcag agttgaaaaa aggaaaactt attaa
//
LOCUS       DAZFWP020000086        20470 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFWP020000086 DAZFWP020000000
VERSION     DAZFWP020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.086"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..340
                     /gene="ydjO"
                     /locus_tag="ACHK0Z_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             <1..340
                     /gene="ydjO"
                     /locus_tag="ACHK0Z_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HGU4502984.1"
                     /translation="LRFKKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSI
                     PDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKAT
                     APIQNGPLPA"
     gene            complement(344..1735)
                     /gene="tcyP"
                     /locus_tag="ACHK0Z_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             complement(344..1735)
                     /gene="tcyP"
                     /locus_tag="ACHK0Z_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU4502985.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1868..2458)
                     /gene="ydjM"
                     /locus_tag="ACHK0Z_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             complement(1868..2458)
                     /gene="ydjM"
                     /locus_tag="ACHK0Z_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4502986.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2621..3289)
                     /gene="hxpB"
                     /locus_tag="ACHK0Z_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             complement(2621..3289)
                     /gene="hxpB"
                     /locus_tag="ACHK0Z_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU4502987.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3436..3972
                     /gene="yniB"
                     /locus_tag="ACHK0Z_RS15020"
                     /old_locus_tag="pgaptmp_003004"
     CDS             3436..3972
                     /gene="yniB"
                     /locus_tag="ACHK0Z_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU4502988.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4013..4873)
                     /gene="yniA"
                     /locus_tag="ACHK0Z_RS15025"
                     /old_locus_tag="pgaptmp_003005"
     CDS             complement(4013..4873)
                     /gene="yniA"
                     /locus_tag="ACHK0Z_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU4502989.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4979..5269)
                     /gene="ghoS"
                     /locus_tag="ACHK0Z_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             complement(4979..5269)
                     /gene="ghoS"
                     /locus_tag="ACHK0Z_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4502990.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5370..6299)
                     /gene="pfkB"
                     /locus_tag="ACHK0Z_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             complement(5370..6299)
                     /gene="pfkB"
                     /locus_tag="ACHK0Z_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU4502991.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6586..7344
                     /gene="ydiY"
                     /locus_tag="ACHK0Z_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             6586..7344
                     /gene="ydiY"
                     /locus_tag="ACHK0Z_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU4502992.1"
                     /translation="MKLLKTVPAIVMLARGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRITLSLGYSM"
     gene            complement(7397..7504)
                     /gene="yniD"
                     /locus_tag="ACHK0Z_RS15045"
                     /old_locus_tag="pgaptmp_003009"
     CDS             complement(7397..7504)
                     /gene="yniD"
                     /locus_tag="ACHK0Z_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU4502993.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7680..9578)
                     /gene="espL1"
                     /locus_tag="ACHK0Z_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /pseudo
     CDS             complement(7680..9578)
                     /gene="espL1"
                     /locus_tag="ACHK0Z_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10102..12030
                     /gene="thrS"
                     /locus_tag="ACHK0Z_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             10102..12030
                     /gene="thrS"
                     /locus_tag="ACHK0Z_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU4502994.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEMFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12034..12576
                     /gene="infC"
                     /locus_tag="ACHK0Z_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             12034..12576
                     /gene="infC"
                     /locus_tag="ACHK0Z_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU4502995.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12673..12870
                     /gene="rpmI"
                     /locus_tag="ACHK0Z_RS15065"
                     /old_locus_tag="pgaptmp_003013"
     CDS             12673..12870
                     /gene="rpmI"
                     /locus_tag="ACHK0Z_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU4502996.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12923..13279
                     /gene="rplT"
                     /locus_tag="ACHK0Z_RS15070"
                     /old_locus_tag="pgaptmp_003014"
     CDS             12923..13279
                     /gene="rplT"
                     /locus_tag="ACHK0Z_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU4502997.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13396..13520
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13402..13446
                     /gene="pheM"
                     /locus_tag="ACHK0Z_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             13402..13446
                     /gene="pheM"
                     /locus_tag="ACHK0Z_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU4502998.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13730..14713
                     /gene="pheS"
                     /locus_tag="ACHK0Z_RS15080"
                     /old_locus_tag="pgaptmp_003016"
     CDS             13730..14713
                     /gene="pheS"
                     /locus_tag="ACHK0Z_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU4502999.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14728..17115
                     /gene="pheT"
                     /locus_tag="ACHK0Z_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             14728..17115
                     /gene="pheT"
                     /locus_tag="ACHK0Z_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU4503000.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHSLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGKRIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17120..17419
                     /gene="ihfA"
                     /locus_tag="ACHK0Z_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             17120..17419
                     /gene="ihfA"
                     /locus_tag="ACHK0Z_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU4503001.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17520..18500
                     /gene="btuC"
                     /locus_tag="ACHK0Z_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     CDS             17520..18500
                     /gene="btuC"
                     /locus_tag="ACHK0Z_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU4503002.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            18563..19114
                     /gene="btuE"
                     /locus_tag="ACHK0Z_RS15100"
                     /old_locus_tag="pgaptmp_003020"
     CDS             18563..19114
                     /gene="btuE"
                     /locus_tag="ACHK0Z_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU4503003.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19114..19863
                     /gene="btuD"
                     /locus_tag="ACHK0Z_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             19114..19863
                     /gene="btuD"
                     /locus_tag="ACHK0Z_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU4503004.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19941..20405
                     /gene="nlpC"
                     /locus_tag="ACHK0Z_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             19941..20405
                     /gene="nlpC"
                     /locus_tag="ACHK0Z_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4503005.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         5285 a         4760 c         5208 g         5217 t
ORIGIN      
        1 gctgcgattc aaaaaaaatg agagtctcct aaagtttcct tcgataatat caatgcttaa
       61 tatcaatctt ctttttaatc cactaaatct tccgggaatg ggaagtggtg tgcttgaaga
      121 tattatgagt attccagata gttcacttag aaaacgtctc gggtatgagg ttttaagctt
      181 ctcactacag gcacattcat tgagccagga atgtattgat aagttggaca tttttttcgc
      241 tgacgatctg tttaaatatg agtcagtatg catcgccgcg atggaacatt taaaaagtaa
      301 agcaacggcc cctatacaaa acggaccgtt gccagcataa gaattaacgg tgtgccagtt
      361 cggcgtcgtc ttcgctatcc agaatggctt tatcggtttg cttcagccac tggctggtca
      421 gcgtgccagc tgtcatcgag ccactaacgt ttagcgccgt acggcccatg tcgataagcg
      481 gttcaacgga gattaacagc gccaccaggg ttactggcag gcccatcgca ggcagcacaa
      541 tcagtgcggc gaaagttgca ccaccaccga caccggcaac gcctgcggaa ctaacggtaa
      601 caataccgac cagcgtcgca atccacatcg ggtccagcgg gttaatgcca accgtaggcg
      661 caaccatcac cgccagcatt gccggataca aaccggcgca gccgttctga ccaatggttg
      721 caccgaaaga ggcggcgaaa ctggcgatgg attcaggaac gcccagacga cgcgtttgtg
      781 cttccacatt cagtgggata gacgcagcac tggaacggct ggtaaaggca aacgtcagca
      841 caggccatac cttacggaag tacttcagcg gactcacgcc attaatgccc agcagaatgc
      901 catgcactgc aaacataatc agcagaccga ggtaggacgc gacaacgaaa cttcccagtt
      961 tgatgatgtc ttgcaagtta gaacctgcaa ccactttggt cattagagcc agaacgccgt
     1021 aaggggtcaa ttgcatgaca aggcgaacca gtttcatcac ccagctttgt agggtatcga
     1081 tagcggctaa gacgcgttca cctttcggcg catcatcctt cagcagtttc aatgcagcta
     1141 cgccgaggaa tgcggcaaaa attaccacgc tgataattga cgtcggattg gctccggtaa
     1201 gatcggcaaa cgggtttttc gggataaagg acaagaccag ctgcggaacg ctcagatcag
     1261 agactttacc aacatagtta ctttcaatgg cgttcagacg tgcagtttct gcaccaccct
     1321 gaaccagacc ttcagccgtc aaaccaaaca ggttggtgac cagcacaccg accagcgccg
     1381 caatcagcgt ggtaaacaaa agcgtaccga tggtcagaaa actgattttg cctaactgag
     1441 atgcgttatg cagacgggca accgcgctca gaatagaggc gaagactaac ggcataacga
     1501 tcatttgcag cagttgaaca tagccgttac caacgatgtt aaaccactgt acagaatctt
     1561 taagtacctg gctgtcagaa ccataaatgg tatgcagggc aaggccaaaa accacaccca
     1621 ttaccagacc caccagcact tttttcgcca gactccactg tttatggcgg gtctgagcca
     1681 gcgcaaacag cagtacaacg aacaccacga tgttcgcaat taatggaaag ttcatccccg
     1741 ttctcctgat cttattattt ctatcactgc gtagcagtca tagtgttggc gcaagattaa
     1801 cagaactgtg agctggctct tatattcaaa tggaattgct tattccagaa aggtgatttt
     1861 tgtcttttta gtattcaacc tgatgcttaa taagccgatg aaactggatc tgcaaggtat
     1921 tgatcatttg cgatgaccaa cgaacagcgc tgttctcggg taatgaatgg ggcatccata
     1981 ccgaccagac aaataatgcc atgcagataa aacgttccag ttggttgccc ttttggggaa
     2041 ccaggatagg caagcggaaa cgccagcggc atggccagag caggggaaca ccggcgggtg
     2101 tcagcatatc ggcaagtatg tggctcaaat aacccagcac cattccttgt agcgcatcag
     2161 ccggaatgaa ccagccttcc ggaaccttaa ggtaaaaggt tgccagcagc gcaaataccg
     2221 ccagcagact gtgggtaaaa ccacgatgcc caaaagcgcg ggcgatcggt tttgatatcc
     2281 attttaatcg ctgcccaaga aacgactttg ggtgatcgat gtccggtaac aaacacgtca
     2341 ggattgcgga agggacaata tgccaccagt caccctgtgc cagcacgggc gtcagctcgg
     2401 catttttggc aaataccgca caagcaatag aaaagagaag gtgaccttcc gccgtcatga
     2461 taaaactcac taaactgtcg atacgtacag tataggattt ttatacagtg cacggaagtg
     2521 gtgacggtgt aactctttct cttaaattga cgattaatcc gtcagttgaa tgcaaattca
     2581 gataaagaac caggggcagc gagatcactg cccctggtca ttaaccgaga aggtcttttg
     2641 cggtgagttc tgtcagcgat gagagtttga cgtttgctaa tacaaaacgt ggatcatttt
     2701 gcgcttctgg cgcaggaacg acaatggaac gcatgcgggc tgctttagag gcgatcatgc
     2761 catttactga atcttccagc gctacgcagg tcagtgggtc aacgcccagt tttgctgcgc
     2821 agtcgagata tacttgcgga tgcggcttgc tgtaaggcag tttttcagcc gaggcgaggg
     2881 catcgaaact gtcgcgtaag tcaaacatgg tcaacacttt ttccagcata tgtagtggtg
     2941 acgcggaggc cagtcccacc aataaacctt gttctttgca taacgcaacg gcttcgcgca
     3001 cgcctggtaa taatggacgt gtctcttcaa ccagtgaaat ggcacgggca ataacccgtt
     3061 ctactacttc ctgacggctt ggcccattcc acggttgccg ggcgtaccaa agatcgacca
     3121 ccatatcgat gcgtaaacct aaggtgtccg gcagctcgtt acgacgggag atatccaccc
     3181 ccaggcttgc catcacatcc agttcggctc gatcccataa aggttctgag tcgataagta
     3241 atccatccat atcaaaaatt gcagcaagaa tctgacgcgg ggttgacatt acaacctctc
     3301 ctttagagca ttatgtgtaa ggtattaagc cgtgcttatg attaagcgcc tatcatatcg
     3361 cctttaaaga atgggcgaaa acagatatct acaggtaaac taagtgaaac gacgcgtctt
     3421 aatcaaggag aactcatgac gtatcaacaa gctggacgca ttgctgtttt gaaaaggatt
     3481 ttgggttggg tgatttttat acctgcattg atctctacat tgatttcttt gctgaaattt
     3541 atgaataccc ggcaggaaaa ccaggaaggc attaatgcgg tcatgctcga ctttactcat
     3601 gtcatgatcg atatgatgca ggcgaatacg ccttttttga atctgttctg gtataactcc
     3661 ccgaccccta attttaatgg tggcgtgaac gttatgttct gggtgatttt catcctgatt
     3721 tttgtcggac tggcgttgca ggattccggt gcccggatga gtcgccaggc gcgcttcttg
     3781 cgggaaggtg ttgaagatca actcattctg gaaaaagcca aaggggaaga ggggctaacg
     3841 cgtgaacaaa ttgagtcccg tattgttgtt ccgcaccata ctattttcct gcagtttttc
     3901 tcactatata tcctgccggt tatttgtatt gctgcaggtt atgtgttctt ttctctgctt
     3961 gggtttattt aatcaaaagg ccgcacggag cggccattat cctcaaccca tatcatgctg
     4021 ctaataatct atccaatgac tgctgagcaa taaccaaatg ctgaccgcca aataatcttg
     4081 cacgatttaa cagcgtgtag agttggtaaa ccggttgacg ttcaaggaaa tcggcaggta
     4141 gcggggatac tgactgatag ccgtcataga tttgtggcgg ctgttcagta tgcagcggta
     4201 acatcgccag gtcgcactct cggtcacccc agtagcaggc agggtcgaaa atgtacgggc
     4261 catccggacc cagcgcacag ttgccggacc ataaatcgcc gtgcaacaga gaaggctgcg
     4321 gttgatgcga ggccagacgc tgctgaatat gctcgacgat ggcgtcgata ttgccgaaag
     4381 cgatcccttt ctctgctgcc agttccaact gccagccaat ccgttgttca gcaaaaaacg
     4441 ttgaccagcg acgttgccag gtgttgggct gtggagttgt ggagagcgag ttatcaaaat
     4501 cgaggccaaa ttgtggttgg tcactccatt gatgtaaacg cgcaatttgc tgaccaagaa
     4561 taaatgcgct atgcgcatcc agcggacgag gtgggagata atccatcacc agaaaactgt
     4621 agtcacggtc agcgcctact gcccaaacct taggcacggt gacggtttta ctacgcgaca
     4681 gtaactccag ttggtcggct tcggcggtaa aaccgggaag cagttccctt tcatcacatt
     4741 tgacgaaaaa gtcatgtcct gcatagcgca aatgccatgc ggcatggact tctccgccag
     4801 gcagttcatt acgcagttcg atttcgcctt cacctaactg ctcgctcaaa agacgactga
     4861 ttgcctgcca catgatgtct ctcccatgtt gtctgccaga tcataaggtt agcgcaagaa
     4921 tgcggtgtaa aaacacgagc taacgcacac cacagactga ttattgccca aaaaccattt
     4981 attttctgtt gctattccat tcctcccagg tcgtgatggt gatttcagga tctttgccgg
     5041 ttgcactttg ggtaagcccc gaaaccagtt ctctaatttc ttcttcactt tgggtactga
     5101 taagtccaaa agtgttagtc cccagctcat ggatatttcc ctcatcatcc gtcatggtga
     5161 gcaaaaaacc gtcgcgagtc aggtaattat tcaactcgtt aatttcagtc aatgtatcct
     5221 catgcaacat gacggttatg acataacgga caagatcgcc actggccata gttcacccct
     5281 ttgttatcac atgacttttg agcatagtcg gagaaacgcg ttgccgacag gttggtggtg
     5341 attcccccaa tgctggggga atgtttttgt tagcgggaaa ggtaagcgta aattttttgc
     5401 gtatcgtcat gggagcacag acgtgttccc tgattgagtg tggctgcact ccccgcagct
     5461 acgccaaaac ggaccatctc ttcaagagag gcattttctg ccagtttcag tgtcatcgcg
     5521 ccgaccatgc tgtcaccagc gccaacggta ctctggcttt tcaccggtgg tggcaccacc
     5581 tgaatgcagt tttcactatc aacacccagc gctccttgtg gaccaaggga aacgacaacc
     5641 cgtttggcct tgccgctatt aacgatttcc tgcgcggctt tgcggacatc gtctggctgg
     5701 gtgagttcgc gattcaccag cgcactgagt tctttttggt taggcttaac caactcgatg
     5761 ttaccaatcg ccagtgctgc acttaacgct tcgccagaac tgtcgatgat gcagcggatc
     5821 ccttggtttt gcgcagcgga aatcagttgg gttaattttt ccagcttcac acctggcggc
     5881 aggcttccgc ttatgaccag gatggccccg gattcaattt ccagaacttg ctcttcaagc
     5941 tggcgaaact catcttcatt taatgccgcg cctggcataa caaaacgata ctgctcaccg
     6001 cttgcttcca catgtacgtg taaattctgc cgggtccagt ctttggcttc tacagtagcg
     6061 acggggacat tttcatccgc caacagagaa accaggtgtt cgccggtcgc gccacccgcc
     6121 gggaagatcg ctgtggcact gcctccaaga tgggcaatgg cgcgggcgac gttgatgccg
     6181 ccgcccccgg gttcgaacac cggtgcggta cagcgcagtt ttccttcggg ataaatttgc
     6241 ggggtaattg ttgcgctatc gagagagggc gcaagtgtca acgtatagat acgtaccatc
     6301 atttcctcct ataggctgat ttcagtctgg caccgaatca gccgaaagtg aagaaattaa
     6361 caatatgatt tattagagct ttatttaaaa ttttgcagat aaatatatat aaataaaaat
     6421 ctgttttcgc cacatgctta ttcaaaaaat gaaataagaa tacattgcta agttattcgc
     6481 gcctttttat aattcgttac ctttccaggg acgctaatct ttgatcctta agaaagtttc
     6541 cgggaccgcg atggtctctt tttttgttgt acggactctt gataaatgaa gcttttgaag
     6601 acagttcctg ccatcgttat gttggcgagg ggcatgtttg cgtcactgaa tgcagctgcc
     6661 gatgattccg tttttactgt catggacgat cctgccagcg ctaaaaaacc tttcgaaggt
     6721 aatctgaacg caggatatct tgcacaatca ggcaatacga aaagctcctc acttacggct
     6781 gacaccacca tgacctggta tggacaaacc actgcctggt cgctgtgggg gaacgccagt
     6841 aatacttctt ctaatgatga gcgttcttca gaaaaatatg cggcgggcgg gcgtagccgt
     6901 tttaatttaa ctgattatga ctatttattt ggtcaggcaa gctggctgac agaccgttat
     6961 aacggctatc gagagcgcga tgtgttgacc gcgggttatg gtcgtcagtt tttgaatggc
     7021 ccggtacaca gtttccgctt tgaattcggt ccaggtgtgc gttacgacaa atataccgac
     7081 aatgccagtg aaactcagcc gctgggctat gcctccggtg cttatgcatg gcagttgact
     7141 gacaacgcta aatttacgca gggtgtttcc gtctttggcg cggaagacac aacgctgaac
     7201 tccgaaagcg cattaaatgt tgctattaat gaacattttg gactgaaagt ggcttacaac
     7261 gtcacatgga actctgaacc tccagagtct gcaccggaac ataccgatcg tcggataacc
     7321 ttatcgctgg gctacagcat gtaaaattat ctgccggacc gtgttatgcg gtctggctgt
     7381 cttcgtttgt cactgctcag ccccaaatca ttgctgccca acgtagcaac aacatagcgc
     7441 cacagattgc cagtagcacc accaccatct tccagtgttt tttatcaaag cgtttagtcg
     7501 gcataccagt gtcctgcata tctcttgtgc gtgataacgg agtgtaacaa aagggacagg
     7561 catcaaggga ttttttgtta ttacagccgt cgggtggcgt tgtacacctg atcactgttt
     7621 ctgcgtttta tgaaaaaaat aaaattatgt atcgatatac aaatatcata aatagctggt
     7681 tataaatacg tttttagttc atcagcacta aaactcaggc tttggctgat tgcattaata
     7741 gcagcataat atgtttctac agttttataa tgattatgat gaatggctat atgaacagct
     7801 gacatgtttt catgattttt agcggccaac agtgtgaata attgacgttg actaaaagaa
     7861 tatttttttg caaaagtact cagtgttgat atataagata acacgacgtc tttattaccc
     7921 ttgctcatgg cgatgtataa agcaggggtt ccatgtgctg tcgcgccttt taataagtcc
     7981 atgatgttga ttttgctgag tttatattta acggcgatcc cataaacttt agagaggaac
     8041 cgtgtggcac acaaagggtg attattttcc attgcagcaa ataaccctgg ttcaccatca
     8101 cgtgttttca tggctaaaat atccatcacc atttttttgt ttaaaagttc ttgtgctatt
     8161 ggagtggtta atgcattcag aaagatatcg attatttcat cgaatccata caacatggag
     8221 atatagagtc caggtgtttc gttagcactt ttagtttgca gtaatttaac cagattatct
     8281 tcatgaatga tatgattgta taccagtgac tggatttctt gcataaatat ttttacgttg
     8341 tcagtttgtc cgttcatcat agctagaaat aaagcaggtg taccaacgct attctgcgca
     8401 gttattattt caatgatttc tttttcaggg agtatgggca actgtttgaa tagtgtaata
     8461 agctattgag tctggcctgt ggccattatt aacaacattg ccgatggatg tataggggca
     8521 aatggcattc gtgtcgtgat acccgctaaa atagcaactg gaactccctc aggtaataga
     8581 ggaagaatag caattgcaca gttgcgaaca tacctatcag cccaggtctg agcatcggga
     8641 gctgaactga tgaacttatc aagcgagaga aagcgccgtg ctgtcctgtg ggtgcctctg
     8701 acggcaacgt tggtatcgtt cgggtcgtaa acagacacaa cataatttaa ttgtccatac
     8761 actgactgct ttattcgtag ccttactgac aaagtatgat tgtctatata taaaatcgcg
     8821 gacatatgac tgacattgtt tgccatcatt cgacaaaaga aaatatctaa agcgaagcca
     8881 aaatccttac tcgcgataac gcgactccct gcaggaggat ggcgaagtaa tgctctgaac
     8941 tccgcatccc ggactaaggg gatatgctgc tgtatggatt caacggagga gtaatgttta
     9001 atcaggttga ctttgccttt tccatttctc aatgaatcaa gggcgtattg caatgacaga
     9061 tggcgacaaa aaatagcgtc agaaggagat tgcaaaaaac atgcactacc attgagattt
     9121 actgttgggt gtatagtttt ttctctatgg atttttttag acagtttcat acaatggtta
     9181 cgatcttcag cagttaactg ttcctgttca atataatcca gtatgcttcg caaatggtta
     9241 gctttaatag gcgtggtttc cagtaataag gaaatgaaat cttttttata gtgtaatgta
     9301 atgagtatat tttctgcatg gatgggtgaa gtggcaaagt taatggtaaa aagggcagtg
     9361 gaataacaat tttgtgatat ttttcgacag tttgcggtaa attcctgaat aacacagtat
     9421 gacagcatcg aacttaagtc agaggtatca agtagggtga tgagctggcg cgcaaattta
     9481 ttaataggct ctgatgaagg tgttggtatg tcgctatcga tgtgcaatct atgaggagac
     9541 ttataatgag ttctgataat gatatcgttt tgactcataa tctattcgcc ttatatatat
     9601 ctggaggttt tgaggatgat gattaattat tatcgccaga acgaaatgtg cacgaaaaat
     9661 tgttataaga attgtgtcac ttttatcatt gtgatatgac cacttcgtca ttaatctcat
     9721 gagcgtttaa aatactaccg ccaaatcacg caacgaactt tgctggattc agtttatgct
     9781 gctgtaaatc cgctcgcagt aaacctttca gacgcaccgt gatgttatca gtggttcttt
     9841 aagcgttttg ctggtgtact cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga
     9901 aaggtacaat ctcccttttt ttagtgttaa catcgctcaa ccgggtggaa ggtgagaggt
     9961 taatgtcttt catttgtgta cctgattgcg aaccaattta gcatttgttg gctagatggt
    10021 ttcgcaatga actgttaata aacaaatttt tctttgtatg tgatctttcg tgtgggtcac
    10081 cactgcaaat aaggatataa aatgcctgtt ataactcttc ctgatggcag ccaacgccat
    10141 tacgaccacg ctgtaagccc catggatgtt gcgctggaca ttggtccagg tctggcgaaa
    10201 gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg cttgcgatct gattgaaaac
    10261 gacgcacaac tgtccatcat taccgccaaa gacgaagacg gtctggagat cattcgtcac
    10321 tcctgtgcgc acctgttagg gcacgcgatt aaacaacttt ggccgcatac caaaatggca
    10381 atcggcccgg ttattgacaa cggtttttat tacgacgttg atcttgaccg cacgttaacc
    10441 caggaagatg tcgaagcact cgagaagcgg atgcatgagc ttgctgagaa aaactacgac
    10501 gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa ctttcgccaa ccgtggggag
    10561 agctacaaag tctccattct tgacgaaaac atcgcccatg atgacaagcc aggtctgtac
    10621 ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc
    10681 catcatttca aactaatgaa aacggcaggg gcttactggc gtggcgacag caacaacaaa
    10741 atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa aagcgcttaa cgcttacctg
    10801 cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg
    10861 taccatatgc aggaagaagc gccgggtatg gtattctggc acaacgacgg ctggaccatc
    10921 ttccgtgaac tagaaatgtt tgttcgttct aaactgaaag agtaccagta tcaggaagtt
    10981 aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac
    11041 aaagatgcaa tgttcaccac gtcttctgag aaccgtgaat actgcattaa gccgatgaac
    11101 tgcccgggtc acgtacaaat tttcaaccag gggctgaagt cttatcgcga tctgccgctg
    11161 cgtatggccg agtttggtag ctgccaccgt aatgagccgt caggttcgct gcatggcctg
    11221 atgcgcgtgc gtggatttac ccaggatgac gcgcatatct tctgtactga agaacaaatt
    11281 cgcgatgaag ttaacggatg tatccgttta gtctatgata tgtacagcac ttttggcttc
    11341 gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac gtattggcag tgatgaaatg
    11401 tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag aaaacaacat cccgtttgaa
    11461 tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg aatttaccct gtatgactgc
    11521 ctcgatcgtg catggcagtg cggtacagta cagctggact tctctttgcc ttctcgtctg
    11581 agcgcttctt atgtaggcga agacaatgaa cgtaaagtac cggtaatgat tcaccgcgca
    11641 attctgggat cgatggaacg tttcatcggt atcctgaccg aagaattcgc tggtttcttc
    11701 ccgacctggc ttgcgccagt tcaggttgtt atcatgaata ttaccgattc acagtctgaa
    11761 tacgttaacg aattgacgca aaaactatca aatgcgggca ttcgtgttaa agcagacttg
    11821 agaaatgaga agattggctt taaaatccgc gagcacactt tgcgtcgcgt cccatatatg
    11881 ctggtctgtg gtgataaaga ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt
    11941 aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc
    12001 agccgcagtc ttaaacaatt ggaggaataa ggtattaaag gcggaaaacg agttcaaacg
    12061 gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg
    12121 gaaggcgagc agcttggtat tgtgagtctg agagaagctc tggagaaagc agaagaagcc
    12181 ggagtagact tagtcgagat cagccctaac gccgagccgc cggtttgtcg tataatggat
    12241 tacggcaaat tcctctatga aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa
    12301 gttatccagg ttaaggaaat taaattccgt cctggtacag atgaaggcga ctatcaggta
    12361 aaactccgca gcctgattcg ctttctcgaa gagggtgata aagccaaaat cacgctgcgt
    12421 ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa
    12481 gacgatttgc aagaactggc agtggtcgaa tccttcccaa cgaagatcga aggccgccag
    12541 atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg
    12601 gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg tttattaaca atgcgaagtg
    12661 gaagttatta aaatgccaaa aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa
    12721 accggtaaag gtggttttaa gcacaagcac gctaacctgc gtcacattct gaccaaaaaa
    12781 gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg tttccaaagg cgatctgggc
    12841 ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg tttttaactt tttaattaga
    12901 atatagatac aggagagcac atatggctcg cgtaaaacgt ggtgttattg cacgcgcacg
    12961 tcacaagaaa attttgaaac aagctaaagg ctactacggt gcgcgttctc gcgtataccg
    13021 cgttgccttc caggctgtta tcaaagctgg tcagtatgct taccgtgacc gtcgtcaacg
    13081 taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac gcagcagcac gtcagaacgg
    13141 tatttcttac agcaaattca tcaatggcct gaaaaaagcc tctgttgaaa tcgaccgtaa
    13201 gatcctggct gatatcgcag tattcgacaa agtagcgttc accgctctgg ttgaaaaagc
    13261 gaaagcagct ctggcataag ccagttgaaa gagggagcta gtctccctct tttcgtttca
    13321 acgccatcaa aacattgact tttatcgccg tagccttttc aataaaggtc ttttgaagag
    13381 taccaaaagg taacgcaagc aatgaatgct gctattttcc gcttcttttt ttactttagc
    13441 acctgaatcc aggaggctag cgcgtgagaa gagaaacgga aaacagcgcc tgaaagcctc
    13501 ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa aaagcctgtc tcatcaggtg
    13561 taattgtcgc agccagtctg gacacggcca gcgcggagaa accggagcgt atttaagtac
    13621 gtgagaattt cgagcactgc ccgggaccaa aatggcaagt aaaatagcct gatgggatag
    13681 gctctaagtc caacgaacca gtgtcaccac tgacacaatg aggaaaacca tgtcacatct
    13741 cgcagaactg gttgccagtg cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt
    13801 agataatgtg cgcgtcgaat atttgggtaa aaaagggcac ttaacccttc agatgacgac
    13861 cctgcgtgag ctgccgccag aagagcgtcc ggcagctggt gcggttatca acgaagcgaa
    13921 agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa ctggaaagcg ctgcactgaa
    13981 tgcgcgtctg gcggcggaaa cgattgatgt ctctctgcca ggtcgtcgca ttgaaaacgg
    14041 cggtctgcat ccggttaccc gtactatcga ccgtatcgaa agtttcttcg gtgagcttgg
    14101 ctttaccgtg gcaaccgggc cggaaatcga agacgattat cataacttcg atgctctgaa
    14161 cattcctggt caccacccgg cgcgcgctga ccacgacact ttctggtttg acactacccg
    14221 cctgctgcgt acccagacct ctggcgtaca gatccgcacc atgaaagccc agcagccacc
    14281 gattcgtatc attgcgcctg gccgcgttta tcgtaacgac tacgaccaga ctcacacgcc
    14341 gatgttccat cagatggaag gtctgattgt tgataccaac atcagcttta ccaacctgaa
    14401 aggcactctg cacgatttcc tgcgtaactt ctttgaggaa gatttgcaga ttcgcttccg
    14461 tccttcctac ttcccgttca ccgagccttc tgcagaagtt gacgtcatgg gtaaaaacgg
    14521 taaatggctg gaagtgctgg gttgcgggat ggtgcatccg aacgtgctgc gtaacgttgg
    14581 catcgacccg gaagtttact ctggtttcgc cttcgggatg gggatggagc gtctgactat
    14641 gttgcgttac ggcgtcaccg acctgcgttc attcttcgaa aatgatctgc gtttcctcaa
    14701 acagtttaaa taaggcagga atagattatg aaattcagtg aactgtggtt acgcgaatgg
    14761 gtgaacccgg cgattgatag cgatgcgctg gcgaatcaaa tcactatggc gggcctggaa
    14821 gttgacggtg tagaaccggt tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt
    14881 gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga caaaagtgaa tgttggcggc
    14941 gatcgcctgc tggacatcgt ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg
    15001 gtagctacca ttggtgctgt tctgccgggt gatttcaaaa ttaaagcggc gaaactgcgt
    15061 ggtgaaccgt ctgaagggat gctgtgctcc ttttctgagc tgggaatttc tgacgatcat
    15121 agcggcatta tcgagctgcc tgcggatgcg ccgattggca ccgacatccg tgaatacctg
    15181 aaacttgatg acaacaccat cgaaatcagc gtgacgccaa accgtgccga ctgtttaggt
    15241 atcattggtg ttgcgcgtga cgttgccgtg ctgaatcagc tgccgctggt tgaaccggaa
    15301 atcgttccgg ttggtgcgac catcgacgac acgctgccga ttacagtcga agcgccggaa
    15361 gcctgcccgc gttatcttgg ccgtgtggta aaaggcatta acgttaaagc gccaactccg
    15421 ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac
    15481 gtcaccaact atgtactgct cgaactgggc cagccgatgc acgctttcga taaagatcgc
    15541 attgaaggcg gcattgtggt gcggatggcg aaagagggcg aaacgttggt actgcttgat
    15601 ggcactgaag cgaagctgaa tgctgacact ctggtcatcg ccgaccacaa caaggcgctg
    15661 gcgatgggcg gcatctttgg tggcgaacac tctggcgtga atgacgaaac gcaaaacgtc
    15721 ttgctggaat gcgctttctt cagcccgctg tctatcaccg gtcgtgctcg tcgtcatagc
    15781 ctgcatactg atgcgtctca ccgttatgag cgtggcgttg atccggcact gcagcacaaa
    15841 gcgatggaac gtgcgacccg tctgctgatc gacatctgcg gtggtgaggc tggtccggta
    15901 attgatatca ccaacgaagc aacgctgccg aagcgtgcaa ccatcacttt acgtcgtagc
    15961 aaactggatc gcctgatcgg ccatcatatt gcggatgagc aggtaactga cattctgcgt
    16021 cgtctcggct gcgaagtgac cgaaggcaaa gacgagtggc aggcagttgc gccgagctgg
    16081 cgtttcgata tggagattga agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac
    16141 aacaacatcc cggatgagcc ggtacaggca agcctgatta tgggtactca ccgtgaagct
    16201 gacctgtcgc tcaagcgcgt gaaaacgctg ctcaacgaca aaggctatca ggaagtgatc
    16261 acctacagct tcgttgatcc gaaagtgcag cagatgatcc atccaggcgt tgaagcctta
    16321 ctgctgccaa gcccgatctc tgttgaaatg tcagcaatgc gtctttctct gtggaccggc
    16381 ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga accgtgtgcg cattttcgaa
    16441 agcggtctgc gtttcgtacc agatactcag gcaccgttgg gcattcgtca ggatctgatg
    16501 ttagccggtg tgatttgcgg taaccgttac gaagagcact ggaacctggc aaaagagacc
    16561 gttgatttct atgatttgaa aggcgatctt gaatccgttc tcgacctgac cggtaaactg
    16621 aatgaggttg agttccgtgc agaagcgaat ccggcactgc atccggggca atccgcagcg
    16681 atttatctga aaggtaaacg tattggtttt gttggggttg ttcatcctga actggaacgt
    16741 aaactggatc ttaacggtcg cactctggtg ttcgaactgg agtggaacaa gctcgcagac
    16801 cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg
    16861 gtggtggtcg cagaaaacgt tcccgcagcg gatattttat ccgaatgtaa gaaagttggc
    16921 gtaaatcagg tagttggcgt aaacttattt gacgtgtacc gcggtaaggg tgttgcggag
    16981 gggtataaga gcctcgccat aagcctgatc ctgcaagata ccagccgtac actcgaagaa
    17041 gaggagattg ccgctaccgt cgccaaatgt gtagaggcat taaaagagcg attccaggca
    17101 tcattgaggg attgaaccta tggcgcttac aaaagctgaa atgtcagaat atctgtttga
    17161 taagcttggg cttagcaagc gggatgccaa agaactggtt gaactgtttt tcgaagagat
    17221 ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct ggttttggta acttcgatct
    17281 gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg ggcgaggata ttcccattac
    17341 agcacggcgc gtggtgacct tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc
    17401 ttcgcccaaa gacgagtaat ctgatctaac taaaaaggcc gctctgcggc cttttttctt
    17461 ttcactgtcg aagagtcacc gtaaaatcaa cgccatgaca cttcagcaga acggatacca
    17521 tgctgacact tgcccgccaa caacagcgac aaaatattcg ctggttatta tgcctgtcag
    17581 ttttgatgct gctggcgctt ctcttaagcc tttgcgccgg tgaacaatgg atctcgccag
    17641 gtgactggtt tactcctcgt ggcgaactgt tcgtctggca aattcgcctg ccacgtacgc
    17701 tggctgtatt gctggttggt gcggcgctgg ctatatccgg cgctgtaatg caggcgttgt
    17761 ttgaaaatcc tctggcagaa cctggactac ttggcgtctc taacggcgca ggcgtggggc
    17821 ttatcgccgc ggtattgctt gggcaagggc aactccccaa ctgggcgcta gggctgtgtg
    17881 cgattgctgg cgcgcttatc atcactttaa tactcttacg tttcgcccgt cgtcatcttt
    17941 cgaccagtcg gttattgctg gctggcgttg cattagggat tatctgtagc gcactaatga
    18001 cgtgggctat ctacttttcc acctctgttg atttacgtca gctgatgtac tggatgatgg
    18061 gcggttttgg cggcgtagac tggcggcaaa gctggctgat gctggcattg atccccgtgt
    18121 tgttgtggat ctgttgtcag tccaggccga tgaatatgtt agcacttggc gagatctcgg
    18181 cgcggcaact gggtttaccc ctgtggttct ggcgcaatgt gctggtggcc gcgaccggct
    18241 ggatggttgg cgtcagtgtg gcgctggcgg gtgctatcgg ctttattggt ctggtgatcc
    18301 cacatattct tcggttgtgt ggtttaaccg atcatcgcgt attacttccc ggctgcgcgc
    18361 tggcaggggc gagcgcattg ctgctggccg atattgtagc gcgcctggca ttagctgccg
    18421 cagagctgcc tattggcgtg gtcaccgcaa cgttgggtgc gccggtgttt atctggttat
    18481 tgttaaaagc aagacgttag ccgcaaaaag acggtctatg attaaaagct aagattttta
    18541 ctgaccacac ccaggagaaa cgatgcaaga ttccattctg acgaccgtag tgaaagatat
    18601 cgacggtgaa gtgaccacgc tggagaagta cgctggtaat gtgctgttga ttgtcaatgt
    18661 cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg gagaatattc agaaagcctg
    18721 ggccgatcga ggttttgtgg tgctgggatt cccgtgcaac cagtttctgg aacaagaacc
    18781 gggcagcgat gaagagatta aaacttactg taccaccaca tggggggtga cgttcccgat
    18841 gttcagtaag attgaagtta atggcgaagg acgccatccg ctgtatcaaa aattgattgc
    18901 cgcagcgccg accgcagtcg cgccggaaga gagcggattc tatgcccgta tggtcagcaa
    18961 aggtcgtgca ccgctgtacc ctgatgatat tttatggaat tttgagaaat ttttagttgg
    19021 cagggacggg ttggtcatcc agcgtttttc cccggatatg acgcctgaag atcccattgt
    19081 gatggaaagc attaaactgg cgttggcaaa ataatgtcta ttgtgatgca gttacaggat
    19141 gttgcggaaa ctacccgcct ggggccgctt tctggcgagg ttcgggctgg ggagatcctg
    19201 cacctggtgg ggccgaatgg cgcgggtaag agtaccttgc ttgcacgaat ggccggaatg
    19261 accagcggta agggaagcat tcagttcgcg gggcaaccac tggaagcatg gtccgcaaca
    19321 aaactcgcgc tgcatcgcgc ctacctttca caacagcaga cgccgccgtt tgcaatgccg
    19381 gtctggcact acctgacact gcatcagcac gataaaacgc gtaccgaact actgaatgat
    19441 gtcgcagggg cgctggctct tgatgacaaa ctcggacgta gcaccaatca actttccggc
    19501 ggtgaatggc aacgcgtacg tcttgctgcg gtggtgctgc aaatcacacc acaagccaat
    19561 cccgcaggtc aattgctgct tcttgatgag ccgatgaaca gtcttgatgt tgcgcaacaa
    19621 agtgcgttag acaaaattct gagcgctctg tgtcagcaag gactggcgat tgtgatgagc
    19681 agtcacgatc tcaaccacac attgcgtcat gcgcatcggg cgtggttgct aaaaggtgga
    19741 aaaatgctgg ccagtggacg cagagaagag gtgctcacgc cgccaaatct ggcgcaggcc
    19801 tatgggatga attttcgccg tctggatatc gaaggtcaca gaatgctgat ttcgaccatc
    19861 tgatttcatt agtatcttga aaatcgggta attacgacgc taaattaatg caagaataaa
    19921 aaacagagga accgtacgga atgcgtttct gccttatttt gatcacagca ctgtttctgg
    19981 ccgggtgtag ccaccataaa gcaccgccgc caaatgccag actttctgat tcgattaccg
    20041 ttattgccgg tttgaacgac cagctacaaa gctggcatgg cacgccgtat cgttatggtg
    20101 gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt tgtaacgatg cgcgatcgtt
    20161 tcgatttgca gctgccccga gaaaccaaag aacaagcctc tatcggcacg caaattgata
    20221 aagacgagtt gctgcctggt gacctggtct ttttcaaaac gggttccgga caaaatggtt
    20281 tgcatgtagg tatttacgat accaacaacc aatttatcca cgcctctacc agcaagggag
    20341 tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa
    20401 tttagtatgt cgaaaaatgg acggcgatga tacatcgccg tccatttact cctgaataca
    20461 gtagtatttt
//
LOCUS       DAZFWP020000087        20444 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFWP020000087 DAZFWP020000000
VERSION     DAZFWP020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.087"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            108..356
                     /locus_tag="ACHK0Z_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             108..356
                     /locus_tag="ACHK0Z_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4503006.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            358..2448
                     /locus_tag="ACHK0Z_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             358..2448
                     /locus_tag="ACHK0Z_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4503007.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKVDESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            2519..3451
                     /locus_tag="ACHK0Z_RS15125"
                     /old_locus_tag="pgaptmp_003025"
     CDS             2519..3451
                     /locus_tag="ACHK0Z_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4503008.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            3454..3675
                     /locus_tag="ACHK0Z_RS15130"
                     /old_locus_tag="pgaptmp_003026"
     CDS             3454..3675
                     /locus_tag="ACHK0Z_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503009.1"
                     /translation="MAVFYIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            3688..3942
                     /locus_tag="ACHK0Z_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             3688..3942
                     /locus_tag="ACHK0Z_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503010.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            3944..4225
                     /locus_tag="ACHK0Z_RS15140"
                     /old_locus_tag="pgaptmp_003028"
     CDS             3944..4225
                     /locus_tag="ACHK0Z_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HGU4503011.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            4222..4494
                     /locus_tag="ACHK0Z_RS15145"
                     /old_locus_tag="pgaptmp_003029"
     CDS             4222..4494
                     /locus_tag="ACHK0Z_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503012.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            4499..4792
                     /locus_tag="ACHK0Z_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             4499..4792
                     /locus_tag="ACHK0Z_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503013.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            4804..5334
                     /locus_tag="ACHK0Z_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             4804..5334
                     /locus_tag="ACHK0Z_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4503014.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            5432..5974
                     /locus_tag="ACHK0Z_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             5432..5974
                     /locus_tag="ACHK0Z_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503015.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            5978..6511
                     /locus_tag="ACHK0Z_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             5978..6511
                     /locus_tag="ACHK0Z_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503016.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            6511..7026
                     /locus_tag="ACHK0Z_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             6511..7026
                     /locus_tag="ACHK0Z_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503017.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQTVKDEAKRLEAVLNVDCRSVFV
                     SGINGFYFKGLRFTDNKYPFHRDLWRKPTASNGWSCTPRTSCIPKALCAASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVEWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            7030..7581
                     /locus_tag="ACHK0Z_RS15175"
                     /old_locus_tag="pgaptmp_003035"
     CDS             7030..7581
                     /locus_tag="ACHK0Z_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4503018.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHKRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDVVLRELMP"
     gene            7578..7889
                     /locus_tag="ACHK0Z_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             7578..7889
                     /locus_tag="ACHK0Z_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503019.1"
                     /translation="MITTLFVESDEPLVCAAGMSVCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            7904..8254
                     /locus_tag="ACHK0Z_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             7904..8254
                     /locus_tag="ACHK0Z_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503020.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            8270..8602
                     /locus_tag="ACHK0Z_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             8270..8602
                     /locus_tag="ACHK0Z_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503021.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            8595..8792
                     /locus_tag="ACHK0Z_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     CDS             8595..8792
                     /locus_tag="ACHK0Z_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503022.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            8782..9078
                     /locus_tag="ACHK0Z_RS15200"
                     /old_locus_tag="pgaptmp_003040"
     CDS             8782..9078
                     /locus_tag="ACHK0Z_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503023.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            9075..9584
                     /locus_tag="ACHK0Z_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             9075..9584
                     /locus_tag="ACHK0Z_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HGU4503024.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            9654..10079
                     /locus_tag="ACHK0Z_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             9654..10079
                     /locus_tag="ACHK0Z_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGU4503025.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            10151..10651
                     /locus_tag="ACHK0Z_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             10151..10651
                     /locus_tag="ACHK0Z_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4503026.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            10686..11114
                     /locus_tag="ACHK0Z_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             10686..11114
                     /locus_tag="ACHK0Z_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503027.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGDDPR"
     gene            11098..11316
                     /locus_tag="ACHK0Z_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             11098..11316
                     /locus_tag="ACHK0Z_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503028.1"
                     /translation="MTIPAEWLADCLVPPVPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            11327..11554
                     /locus_tag="ACHK0Z_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             11327..11554
                     /locus_tag="ACHK0Z_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4503029.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            11535..11846
                     /locus_tag="ACHK0Z_RS15235"
                     /old_locus_tag="pgaptmp_003047"
     CDS             11535..11846
                     /locus_tag="ACHK0Z_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HGU4503030.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTN
                     E"
     gene            11839..12129
                     /locus_tag="ACHK0Z_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     CDS             11839..12129
                     /locus_tag="ACHK0Z_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4503031.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            12132..12713
                     /locus_tag="ACHK0Z_RS15245"
                     /old_locus_tag="pgaptmp_003049"
     CDS             12132..12713
                     /locus_tag="ACHK0Z_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HGU4503032.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKV"
     gene            12713..14377
                     /locus_tag="ACHK0Z_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             12713..14377
                     /locus_tag="ACHK0Z_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503033.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            14377..15966
                     /locus_tag="ACHK0Z_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             14377..15966
                     /locus_tag="ACHK0Z_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HGU4503034.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVSWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLCDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            15950..17281
                     /locus_tag="ACHK0Z_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             15950..17281
                     /locus_tag="ACHK0Z_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HGU4503035.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQTRLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            17403..17876
                     /locus_tag="ACHK0Z_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             17403..17876
                     /locus_tag="ACHK0Z_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4503036.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            18053..19177
                     /locus_tag="ACHK0Z_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             18053..19177
                     /locus_tag="ACHK0Z_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4503037.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGIIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            19177..20124
                     /locus_tag="ACHK0Z_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             19177..20124
                     /locus_tag="ACHK0Z_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4503038.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            20168..>20444
                     /locus_tag="ACHK0Z_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             20168..>20444
                     /locus_tag="ACHK0Z_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HGU4503039.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTD"
BASE COUNT         4953 a         5057 c         5929 g         4505 t
ORIGIN      
        1 caaaatacca aaaatacctt gatttggtat ttttagtgat gcattattta cccgtgcaga
       61 tatccgcacg aatatccgcg cgggtaactt tttagaatag aggaagtatg agtagaagag
      121 aagtttcaga tcacgattgg ccgcctgaaa tcatcaaggc gcgcttacac atggctggct
      181 tatcactgcg ctctttatct ttaaaggctg gttacagcag ggattcgctg aaaagcgtat
      241 tacgtactcc ttgccgaccg tatcagcaaa taattgctga tgctcttggg gtatcgcctg
      301 aagaaatctg gcccagtcga taccaggtta agagctatat gagaaaggcg tcatgatatg
      361 tatgtcattg caaaagaact gattggcgcg cccggaatgc ctgctacaac aaaaggtatt
      421 cgccaggcat tacaacgtta cgtacaaggg aaaagctgtt gttcccgtcg tcgctcaggc
      481 tctaaagcaa ctgaatacag catcgactgt ttacctgaag tgacgcagca ggcattacgt
      541 gaacgctacg ccctgcaact gatgactcaa aaagtcgatg aatcaccggc tccagtggtg
      601 acaaaggcca gacgctcgcc tgccgtggtt gatgcggtgg aggcatatcg cggatcacca
      661 caactgatgg tcgaacgcct taatgccctg actgaaaacc agcgccaggt ggctgatgca
      721 cgaatcgcga tcgtcagcga agttctgaaa gtcgcgcaac aacctggttt cagctgtgcg
      781 aaggctatcc ggtttatcgt tgacaacctg gcacgttcgc agctggacga gcgcattgtg
      841 gcaatggttg agacggcgaa cgccaaaaag ggaaacagcc gcgcgttaag tgaaatcacg
      901 ctgaaacgct ggattgcggc ctttaacaag gcgcagaacg ccgctgaacg cctgctttta
      961 ctggcaccgg gtaagcgcca ggaaataaaa gccgaagata ttaactggct gcccgaattt
     1021 ctggcgcagt atcgccagtc aaacggccga ccaatgaccg aggcttacga ggattttgtc
     1081 gctgaatggc agcaccggca cgctgatgag ccttatatgc tcgatatcat gccgtcttat
     1141 gacaccattc gccgcgcaat gaagaaactg ccggaagtgg tgaaacaaaa aggccgggtg
     1201 accggcagtg aataccgcca gcttgaggga ttcacgcgcc gcgactggtc aaaaatgccg
     1261 gtgaattatg tctggattgg tgacggtcac ggcatgaagc tgaaatgcgc acacccggtt
     1321 cacggtcggc catttgcacc ggaagtgacc tttgttatcg acggtggcac gcgctttgtg
     1381 gtgggctgga gccttgacct ggctgaaaat gttttcgccg tagccggtgc catacagcac
     1441 ggcattcgcc atcacggcaa accgtttctg tattactcgg ataacggctc cggggaaacc
     1501 gccgacatcc tggataagga ggttgtgggg atactgccgc gactggggat taatcacccg
     1561 accgggattg ccggtaatcc gcagggacga ggcattatcg aacggcttaa ccgcacatta
     1621 ccgatgcgca tagcccgtaa ataccgcacc tatttcggga aaggtgcaga tcgcgagacg
     1681 ttacgcaaaa ccaaccgcga tttacgctcg gcatttactg ccctgcaaca gggcaaacgg
     1741 ctgaacgccc ggcagcagtc agcgatgcgt gatttaccgt cctggtctga actgattgat
     1801 gccattcgtg atggtgttga gtggtacaac aaccggccgc acgatgaatt accggtgaag
     1861 ccgaacggca agcattacag cccggcggag ttcagaaaaa aacgcctggc ggaagaggac
     1921 acggaaattg aatggctgtc cgatgtggaa ttgcgggaca tgttccggcc gatggtggaa
     1981 cgccctgtaa gacgctgtga aatacgctgg ctgaataata tttactacgc gcccgagctg
     2041 cgtgatgagc atggccgcaa ggtgcttatc agctatgaca ttcatgatgc cgaacgaatt
     2101 accgtacgtc gcctggatgg cagcgtgatt tgcgaggcgg tatgggacgg taataaacgc
     2161 gaagccttcc cggtcagcgc ggaatactac aaacagcagc aacgccttaa aggcatgcgt
     2221 aaacgcgcag aggaaaaaat ccgtgatgcc gaggatgagg ttgtcaacgt gctggagcac
     2281 aagccgcagg agccctggct ggaaaacatc tatcgcccgg taggtaatac ggtggccgtt
     2341 cagcaacctg ccgttgatga tgagcctgat gaagaatacg agcgcaattt ccagcgggga
     2401 ttgcagttgc tcgaagcgaa attaaaagaa aatgacccgc tggcctgaaa taaaaaataa
     2461 cccgagcggc gactcaggtt atttgattaa acaaggtatc aaatgagagg ttaataatat
     2521 gactgatatt aacgatgtaa tcaagaccat tgatgaactt attgatggcg gcgtactgac
     2581 gcagtatgcc atcgccagag aggcgggaat ttccgacggc acattatcgg ctttccgcaa
     2641 gggaaaatat aaaggcgata acgccgctgt ggctgcttcc ctgcgttcct ggtatgagaa
     2701 ctggaataaa caaagcgcac tgccggaacc gccgcagttt gtggaaacgc agacagttca
     2761 ggagctgcgc gcactgtttc aggcggttcg cctgatgggc tgtattaacg tcattgtggg
     2821 cgtgccgggg gtaggtaaaa cggccaccgc ccgtaattac tgccaggagc aaccaaacac
     2881 ctggatgatc accctgtcac ccgcgcactc cagcgtcacg gagtgtctgc tggagctggc
     2941 cgatgcgctg gggattgatt acacccgcgc gaacaaaggg gcattatccc gcgccatccg
     3001 ccgtcgcctg atgggaacgc gtggactggt gattgtggat gaggcggatc atcttggtat
     3061 tgacggtctg gagcaactcc gggcaattca ggacgccacg gggatcggga tggtgcttat
     3121 tggtaacccg cgcggattgt ttaaaggtgg acgccgcgcc tttgatgatt tatcgcgcct
     3181 gttcagccgt cttgcccgta caaaacaact tcgcaaggcc aaaaaggcgg atgtgctggc
     3241 cattgccagg gcatggggta tcagtggtga ggccgagctg gccgtcatgc aggctatcgc
     3301 tgaaaagccg ggagcgttac gcgttctgac acatacgctt aaccaggcgt ggctcaccgc
     3361 cagcggtgaa ggtgcggcgc tgacagaaaa acatattaat gcggccttta aagaggttta
     3421 taccaaccct gaattactct cacaggtgtg attatggcgg tattttatat tcctgatatt
     3481 tacggacgct tttacctggt taatttcgat aacgtgaagg tgatttcact ggccgaaaat
     3541 aaagaatgtg gcgatttact ttttgaattt aatgaccgca cacgaatggt gatatctgcc
     3601 ggacttgatc gcgaaggtgc gacagaagtt tacagcggaa tatgccgttc tgttggtgcg
     3661 aaacaagtca gctaaatgag gtgttatatg aatatgcaat cctgcggtaa caaaatgaat
     3721 ttattcgact ccctgaacag cgcgcgccgt ctgaccgaac ttgccggtgc ggtactggaa
     3781 cgcagtaagc gctacccaca acgttttgca ctgaaaacca cgccgccggt aggcaacgtg
     3841 cagggaaccg gtgaaattga aatcaccata cagaccaacg gcctgcgccg ccgtgtgaag
     3901 gccacccgca tcagcggctg cacggtttac tgggaggtgt gaggtgaaaa aaaatctcat
     3961 tgcatgggcg tggtcgagtg gtcttattga gtttggttac gtcctgccgg aaggtgcatt
     4021 gccgatagtt gccggaaagc ctgccacggt acggcatgtg attgaggtta tggcgcgtca
     4081 tggacgtgat gaacaggagc agttactggt tccggggata ccggaagcgg tgacggagga
     4141 agaagccttt aatgccatga ttcggttctg ccgtgaggtc agacgccggg tcagttatcc
     4201 aaacagaacg aggaccagag gatgagtaaa gtcgtacgca ttattttcga atacaaggag
     4261 cacgttatcc ataaaaacgc tgatggaaca gtgcgcatgg gggtaagtct ggacatacgt
     4321 tcaaccggga taaagcagaa aggtgatgga cccgccatga tttttggggt ggttatgctc
     4381 gcggaaagca gagactttgc tgaacttgtg gcaatgaaag ccagtgcgct catgaaagat
     4441 atgagcatgc gttccggggt tattaaaggt aatgaattta atcagcaggg gtaattccat
     4501 gagcaaagta cgcgttattt ttgaatttaa gcatgtttcg catgacgaaa aaccggcagg
     4561 caatgactgt gttgaagtgc atgaaaagat tggtgtggat gtaaaaacag aacgtgatac
     4621 gaataacagg ccgacgtcac tctgtgacgt ttatgcaagt attctccagt atcacagtcc
     4681 tgaaattatt cagtttctct cagcggaatt tcaggcatct gtacaggctt ttggggcgga
     4741 tgccatcatt aaacgccacc gcgtgcataa agcatcaggc acactgcaat aaggaaaaac
     4801 aaaatggcaa aacgcgttac aaaattaaag gccgcagcag aggcggcacc gcagacccgt
     4861 gaagaggtca gccgcgatat ccgcaccctg ggcgatattc agcgagaggc gctgcgcctg
     4921 gaaacagcga tgaatgatga agtggcagaa atcaccgccc gttatacgcc gcagattgaa
     4981 aaccttaaaa aagaaatcaa agtgcttttt aaggggattc aggactggtg caaaaccaac
     5041 cgtgatgagc tgacgaacgg cggcaaaacc aaaactgcca atctgaccac cggaacggtg
     5101 tcatggcgac tggggaatcc atcatgcagc gtcagtcgtg atgtggaagg tgtgattgaa
     5161 atgctgcgcc gtatgggcct tgagcgtttt atccgcacga aagaggaagt gaacaaggaa
     5221 gcggtcctgg cggagccgga tgcggtgaaa gggattgccg gtattaaggt gaataaaggc
     5281 gctgaaagtt tttatgtcga gccttttgaa caggacgccg gactgaataa ataacaccgc
     5341 attaatcaat taaatatcac ttcgttttaa ttatggcgct cgcgtcaggg gactgcctgc
     5401 gcctgtaaac tgaaaataag gaacaggata tatggcatat ttttatttca aattagaccg
     5461 tgtgcagaca aataaatatt tcaccaaata tcaacagact gttttaccgc tacgcaacag
     5521 tattcttcgg gcattactga aaaatacagg cgctgccgga ttgcgcttaa agccgtttgc
     5581 catggacgta atcagtgagt tttatttttc tggtgctctg cctgcgggct ggcgtaagcg
     5641 cgatgatgtg gcttttatcg gggacggacc gtgctttatt gccagacctg atgagtcatg
     5701 ccctgaagga ccggcgattg ccgcaatgat tgaagccgct gaacgcgagt taagaaagcg
     5761 tcctgatttc cttgtctggc tctgtgaaaa gctgggggta atgagaatcc cctccatgtt
     5821 taacatggac tcctggtgga ccccgtcgct ctcccgtgat gccctgtgcg tggtgtttaa
     5881 agtaggcgct tatggcaggg aaataaaagg gggtattcct gaagaatgcc aggagattaa
     5941 acattctgaa tatgtggcgc tgacggagga ataattcatg attgatgcaa aagtgcttga
     6001 aggggttaaa aactggctga gtatttacgg gcgtctgacc tgcggcattc tggctgaaaa
     6061 aatgaatatg ccgccatcct cgatggttta ttttctgcgt gatgcggtcg atgccggggt
     6121 gctgacggaa tgtaacggtt tttatgatat tccgcgtccc cgcccggtgc agccggttcg
     6181 tcgcaaatgc agccaggagt ctgcggctga tgatgttcag tggtgcagct tcagaaaatc
     6241 cctgccgtgg attgaagggc atgatattcc gtcgatggcg tgggaatttg ctcagggcgt
     6301 gctgacctgt gaaaccgttt atgtggtggc tgaagttgat gagcaggcca tgaaagaagg
     6361 cgtgccccag tttgtgatgg cgtatatcga catccgcctg ggcgtcatta tctgcggttt
     6421 aagcggctgg aatatcacct cgcgcgttct gcgttacctg attgttgacc ggacggccgc
     6481 gcctgccggg atatcagcgg aggtggcgta atgttcttta aaacatcaaa cccttccgcg
     6541 ctggctgcgt ggcaaaaata ccagcaggac tgccagacgg taaaagatga ggcgaaacgc
     6601 cttgaggccg tgctgaatgt tgattgtcgg tcggtatttg tatccggtat caatggtttt
     6661 tattttaaag gactgcgttt tacggataac aaatatcctt ttcatcgcga cttatggcga
     6721 aaaccgactg cgtcgaatgg ctggagctgc acgccgcgca catcgtgtat tcccaaagcc
     6781 ctgtgcgctg cctctgacga acttaacagt ctgtggcgtg aatattcgcc cgtcacgtat
     6841 gccagaaccg atgccctgtt gttctggctg ggtattgatt tctcggcaat cctgtttggc
     6901 cctgtggagt ggttctgcgt tgacgatgtg atttaccttc agtgcggcgt aaaacccgca
     6961 aaacagaaaa tgaccgaaat tctgtctgat gagttttatg ctgctgaaaa gcgagtcagg
     7021 gggtgatgta tgatgatttt acaacccatg gggcgaaaag gccgtgcacc cgctcatgtc
     7081 cgtgcgtgga cacctgaaga agatgcactg ctgattgcgc tttatccatc caccccggtt
     7141 aaggatattg ctgtcagggt aaaaagaagt ttctggggtg tacataaccg gattgtttta
     7201 ttacgcggta cttacccgga gttgctcaaa tgcaaacgtc caagatttaa acatgatgaa
     7261 gataaattta tccgaaaaaa tgccagaacg atgaccggta agcaaatagg agaatatctg
     7321 gggcgagacc gggattctgt ccacaaacgg gcgcgatata ttggcgtaag catgaaaaaa
     7381 tacggagagt tgctgccctt cacccgtata cctgatgatg atgttcatct tattcgtgaa
     7441 ttacgggatg ctgaatcacc acgacgtctt accttccggg aaataggcga gaaatttgaa
     7501 ttatccgaga gtacggtgaa ttttatttat caccatcgcc ggactgccga agatgttgta
     7561 ttacgggagt taatgccatg ataacgacat tatttgttga atcagacgag cctctggtat
     7621 gtgccgccgg aatgtcggtt tgtggcggaa cgctgaccgg tgtttatttc ggggatttgc
     7681 gcggttatcc ctggcattca ctggatgacg catttccgcc tgatatggag gctgtcgtgc
     7741 tgattgttca gtatggtgac caacaggaac tgcgcatcgg ccatatgggg tatgaaggct
     7801 tttttattga tgaagaaacc ggagcctgcc ttgaagatga ggacggacag gtgacgcact
     7861 ggtgccatat ttcagcttta ccggaatgac gggaggtgaa tgcgtgagaa gttatattga
     7921 taatgaaaaa ctgaagacaa tcagcgactg tcttagcttg ctggcaaaaa taaaagaaac
     7981 cattgaagag attaaatttc agcttgaata cgaaccctgt ggcgatgata catggcgtaa
     8041 ctctgccaga aaagcactgg ctgtctttca gaaacaacgc cggactgttg aataccgcct
     8101 tgctgtattg cgccaggagg aaaaggaaag aaatataaga cgtcatgaac tcgttaatga
     8161 ttttcttgtt cgtgaattaa aggagcgtgt gccggagtcg gtattttttg agtgtgaggc
     8221 tgttgcccgt agcaaagcac tggaaactga ttaaacaggc aggtgaatga tgagtattga
     8281 attaagatcg tcttatgaat atcgtaaaat tctcatcgcc ggaggcatga aaccggaaga
     8341 tgcagaaaaa atcgtttctt ttatggataa agaatgtgac aaacgggata tgccagaaat
     8401 tattatggat gacatgattc tgcattcagc cgtagcgtta agcccgttat ggattgtgca
     8461 ttctcttgct gaaattgcca aaggcaccga taaacaggca gctgttgctg cccttcagac
     8521 gcttaatgaa atgcgtatat ctccacgccc gacattaata catatgattc tgtccagcat
     8581 ggaggataaa gcaaatgaat agtctacccg ctggatgggc gcggccacta atggccagga
     8641 agcatcattt ttttaaaaca ggcgaaaata tcagtatatg tggacggtgg ttatatctgg
     8701 ctcataatcg cgagccggat acatttgaaa gccctgatga ctgtgccgaa tgccgcagaa
     8761 gagtgaataa ggagaaagat aatggacaat agttctctgt tgttctggtg cttatatatc
     8821 acatcctttt tcggcgcatt tgttattacc cggtggttat gtcgaaaaat catctgcttt
     8881 tttgataaaa gacatccggt tgaacgggcg gctgatgcgc tcattcagca ggccattgtg
     8941 ttatatagcg gtgaattttt ctgccggata acaaccagag atggctggca cataatgatt
     9001 attccaccaa cacaccatgc ccggtgggat gaggcagaaa aggctttcca tgtccgtaaa
     9061 aaggtaaata cggtatgaga ggaaaactga tatccgccat tcatgtggca aagcgcgagc
     9121 ttgccctgga tgatgagact tatacatccg cgctgctggc agccaccggc aaaaccagct
     9181 gccgggatat gtcaccggat gagttatccc gcgtgctgga tgttttcaaa aaacgcggtt
     9241 ttaaagtgcg tcagaacccg gttaaccggg ccttaaaacc gggtacggtg accgccaaaa
     9301 ttcgcgccat ctggaaggtg atgcaccggc agggctttat caccgatggt gcggaaaccg
     9361 cccttaaccg ctgggtgaaa tcgcagacgg ccgcgcagaa cggcggcgaa ggtgtggcaa
     9421 actggcagtg gctggagcaa caccccgccc tggcctcaga tgtgctggag cgtctcaagc
     9481 gatggcaccg ccgcaaaatg ctggccgcga tgggaatgcc cgaacgcacg ctgatggggt
     9541 atgacgccgt ttgcaggcag tatgaaaaat cacttccccg ttaaccccaa atcccgccac
     9601 aacgcgggat ttttatttta aacttaccgg gaacgcgaga accggaggct gatatggcag
     9661 aaactcagat gagcatgttt ggtggtgaca gtgagcaact gcacgccctt atcgaccgcc
     9721 ttgatgacat ccctgatgat gttctcaaaa agaactggcc gcggaccctg tcagaactgg
     9781 ttgaagtcac cggcgcagaa cttcagcgtc aggggattga gccggtactg gccggtaaac
     9841 tggcacgcaa ggtggctgcg gctcaggcag cctatatggg cggacggggt tattacctgc
     9901 cggtcgggga atctctcttt gccgagctgc gaaacaatga gatattttcg cgctgggacc
     9961 ggggcgagaa aattgaatcc cttcgccgcc attaccggat gtcagaaacc cagatttata
    10021 ccgtcatacg tgaacagcgc cgtctgcatc tggcaagaac gcaaccgcca cttttctgat
    10081 attccgcaaa gcctgccgcc tttccttcac cgttacgctg actcagagaa catcatgaga
    10141 cagcgtaaca atgccaaaac tccccgcacc actgcgtaaa aagctgattg cccttgttct
    10201 ggccggtgcc gggacgttca cgattgccac gcattacacc ggttactggg aagggaaaga
    10261 aaactccacg tatatcgatc ccaccggtac acccaccatc tgttacggcc ataccggccc
    10321 ggatgtgaaa ccgggtatga ccctgacgga tgaagaatgc ctggaactac tggaaaagga
    10381 catgaaatgg gcctttgcgg ccattgatcg gcgtgttcag gtgccgctta cccgtggtca
    10441 gactgtggcg ctggcttcgt ggatcttctg ggccggtgaa acgaacttcc gcaactccac
    10501 gcttctgcgc ctgatcaatg ccgggcagat gcccgcgtcc tgtaagcagt atatccgctg
    10561 gatttattca aagggggtga aactccccgg ccttgaggcc cgccgttcgg cggatgaatg
    10621 gttatgtcgc tacgacttgc cgaaagtctg aaccgcttct ggcgaccgct catgatagcg
    10681 ctgctgtgtg cggtactgct gttacggggt gtcctgtggc tgcggtgaat aatgccttac
    10741 cggccctgat tgccggggcg tgcatggcgg cgctggggat tatcgctgta ctggttgccg
    10801 cgatatgggg aatgcaccag aagacgcagc gccttgaaga caataatcag gtgcttgtgc
    10861 gtgagcggga tgaggcgcgt caggtgctgg caaatcagca gcacaccctg caactcattt
    10921 cacaaatcag tgaggcggcc acgaatgaaa aacaacagaa cattcagcac agcgagggac
    10981 agcagagcgt tgtccgccgg tcgctggcag cggtgcctgc ggcctctgcc cctgttcctg
    11041 atgatgtggc tgatcgggtg cgccgggccg tctgtgaaat acgtgcctgt gaagccggtg
    11101 acgatccccg ctgaatggct ggctgactgc ctggtccctc ctgtgccgga gccgttcacg
    11161 tttggggcat cggtcactta caacctgcaa ctgctggcgg tgatcaagaa ctgcaacgtg
    11221 gacaaggcca gtattcgtcg tctggaggcg cggcgacaac atgaatttac tgatatggcc
    11281 ggaacgcctg ctgttccggc aggaaagacg aagtaaggaa aaaggtatgg atgattcaga
    11341 tcgtgctcag gctgtgatgg agcggggatc agaacgcgcc ctttgtaacc ggctgacacg
    11401 taagcgccgg gcaatggatg ctccgggcag acgtgtctgc gcggactgtg gcggagagat
    11461 ccccgccgca cgtcttgttg ctgtgccgga tgccatccgt tgtgtgaact gccagaacat
    11521 catggaggcc cgtcatgtgg gtcagcatcg ttaaagatta tgttgtgccg atcctttcgg
    11581 cgacggcaac cgctggcggg atattcatgg cgctgatgcg caaaacgttt gtcccccgcg
    11641 aggcctttga aaaactctcc gaccgcgttg agaaggtgga aacccgcctg tcgtcactgc
    11701 cgacagaagc tgaggtcaac cgtctgaatg tggaaatcgt gaccctgcgg ggtgaactga
    11761 aaaccacgaa cgccacgctc cgctctgtct cctatcagaa cgaactgctg ctggagcagg
    11821 cggtaaggaa aaaaacgaat gagtgatttc attactgaag atcagcgtct ggtcattctg
    11881 cggtcgctgg cagattacaa cggtgaactg ggtgaatccg tgctccagga ctgtctggat
    11941 gattacggcc accgcgtgtc ccgcgacacc gttcacaccc acattgcctg gcttgccgag
    12001 cagggactgg tgcgcaaacg cgttctgatt aacggttatt tcatcgcgga actgaccggc
    12061 cgggggcagg atgtggcgga aggccgcgcc agcgttccgg gtgtgaagaa accacgcgca
    12121 agggggtaac gatggacaag ccgacgcgtg gacgcgtgcg caaggtggat ttgctccccg
    12181 acagcatccg taagccactg ctggaaatgc tgcgtgaaaa acgtctgacg caggtccaga
    12241 tacgcgagga aatcaaccgt ctgatccgtg aggcgggcct gccggaagag cagcagcttt
    12301 caccggcggc catcagccgg gaagcctccc gtaatgagct gattgcccgt aacctgcgtg
    12361 atttacgcga gcagacaaaa gccatgatgg ccgaactggg ggacaagcca accggtgaaa
    12421 ccactgcatt gatcctggag atgtcccgcg ccctgatgta ccgccgtctg cgtgccgcga
    12481 ccgaatccct gaacagtgac agcgacgtgg atatgcgtct tattaaggac atcctcctgt
    12541 cagcacagcg tgcggaaagt gccgccgagc gcagcattaa gcgtgaaaaa gaaatcaggg
    12601 ctgcatttgc tgaagagatg gcaaacgccg tcaccgacga gctgcgcggc gtggacggga
    12661 tgagtgaaca gcttgagtcc cgtattaagg ggattctgtt gggtaaagtc tgatgaataa
    12721 cgccgaagaa ctctccccct tactgacgaa taccgtctcc acccgcaaaa ttgatctggc
    12781 cggtgaaaaa gccctgctgg gcgtggatgt gccggacagc cttgatttgc ccggtgatat
    12841 gccggtattt ctggattatc aggcccgctg gtttgaggat gaaagcgagg tctgcatcgc
    12901 ggaaaaatcc cgccgcaccg ggcttacctg ggcggaagcc gggcgtaacg tcattaccgc
    12961 tgcaaaaccg aagcggcgcg gtggccgcaa tgtgttttat gtggggtcaa agcaggagat
    13021 ggcgctggaa tatatttctg cctgtgcact gttctcccgt gcctttaacc agctggcaga
    13081 tgctgatgtg tatgagcaga ccttctggga tcgggataaa aaagaagaaa ttctgaccta
    13141 catgatccgc tttccgaaca gtggattcaa aattcaggca ctgtcttccc gtccgtcaaa
    13201 cctgcgcggc cttcaggggg atgtggtgat tgatgaagcc gcgttccacg agtcactgga
    13261 tgagcttctc aaggcggcaa tggcgcttac catgtggggc gcgcgcgtgc gtattatctc
    13321 cacgcataac ggcgtcgata atctgtttaa tcagtacatt caggaagccc gcgaagggcg
    13381 caaggattac agcgtccacc gcattaccct ggatgatgcc attgcggacg ggctgtaccg
    13441 tcgtatctgt tatgtcaccg gccgcgaatg gtcaccggaa agcgagcaga agtggcgtga
    13501 tgatctctac aaaaacgccc cgacccgtga ggatgccgac gaggaatacg gctgtatccc
    13561 gaaaaaatcc ggcggtgcct atattcctca cgcgcttatt gaaatggcga tgatccgcga
    13621 catcccgatt ctgacgtttg aagccccgga cgacttcatc agccgtgccg catggctgcg
    13681 tgaatcggag gttttaacct ggtgtgaaga acatttaaaa ccgcttttag aggcgttaaa
    13741 tccccgttcc cgattcagct tcggggaaga ctttgcccgc accggagacc tgtcctgttt
    13801 tgtgctgctg gaaatcactg aatccctggc aaaacgcgag gtgtttcgcg tggagctgcg
    13861 caacctgccg tatgcccagc aggagcaggt gatgatgtac atcctgaccc gcgttccggc
    13921 gctggtcggt gcggcgttcg acgccaccgg taacggcggc tatctggccg aagccgcgct
    13981 gctggccttt ggcccggaca tcatcgactg cgtgatgtta tcgccgaagt ggtacggcga
    14041 gtggatgcca aaactgaagg ccgagtttga ggatcagaat atcctcgtcg cccgccatca
    14101 gaccacgctt gatgatttgc gccatgtgaa ggtggtgaac ggtatcccgc aaatcgacaa
    14161 ggggcgtacg aaggaccaga acgcaacggc tgtgaacgcc cgccgccatg gtgattttgc
    14221 cgtggcgctc tgtatggcaa accgggcgtc atacatggag ggcttcatcc tggatgaatc
    14281 agcctgtcag gcgctgccgg agcggtcgcg ggcaatggag ggcggttatc gtgatgacga
    14341 tgaggcatat catgaatttg atcgggggtg ctggtagtgg gacgcataat tgatcttgac
    14401 ggaaaacctt tctcctttga cccggagatg cagagtgccg tgctggatat tccgcagatt
    14461 gccagccgtt atattgaaca tccggcctcg ggtatcaccc cgaaccgggc ggcacagtgc
    14521 cttcgcggag ctgaacgtgg cgatctgatt gcccagtccg atctggccgc tgacattgaa
    14581 gaaaaggata cccacctttt tgcagagctg ggcaagcgac gccttgccat tcagggggtg
    14641 tcctggagta ttgagccgcc cccgaacgcc agcgcgaatg agaaaaagga cgcggaaatg
    14701 ctcgacgaat atctgcattc cgccgactgg tttgatgcca tgctgtttga tgccaccgat
    14761 gccatcctga agggctattc ctgtatggag attgagcacg ggatgctcgg taaaatgcac
    14821 atcatccgcg ccatccgctg gcgtgacagc gggcatttct gccttaaccc ggatgatttg
    14881 agcgaactgc ggctgtgtga cggcagccat gccggggtgg cgtttcagcc ctttggctgg
    14941 atagtgcatc agtcgcgttc acgcactggc tacggtggtg cgacggggct tgtcagaacg
    15001 cttatctggc cgttcatttt caaaaactat tccgtgcgcg atctggctga atttctggag
    15061 gtgtacggcc tgccgatgaa ggtcggtaaa tacccgtccg gggcaacacc ggagcagaaa
    15121 agcgccctga tgcgggcggt gatggatatc gggcgacgta caggcgggat catcccggcc
    15181 gggatgtcgc tggagtttca ggcggcagcg aacggtcagg ccgatccgtt tgaaaccatg
    15241 atttcgtggg gggagcgttc catctccaaa gctatcctcg gcggcacgct gaccacggaa
    15301 gccggagaca agggcgcacg ttcgctgggt gaagtgcata acgaggtgcg ccgggaaatc
    15361 agggattctg atttacgtca gctggccgcc acgctgaacc gcgatctggt gtatccgctg
    15421 tatgccctga acaccgccca cgccattgat atccgtcgcc tgccgcgtat ctgtttccag
    15481 acaaaagagc cgggggatat caccaaaatc accagtgcgg tgatgcagct cagtacgggg
    15541 atggatattc ctgatccctg ggtgcgggag cagaccggta ttccacagcc tgcccccggt
    15601 gaagccatct tccgtgtccg tcagagtggc aatgaacctg ctcagacaga caaagagatg
    15661 ccaccggaaa aacaggagaa gacagagcag acagcgctgg ctgccagact gccggaagcg
    15721 aagagcagcc cccgagatga actggacgac atgggggatg cggtgcctgc ccgccggtta
    15781 caggaggcca tcgacccggt actggagccg gtcattgaag ccatcagaac gcgggggctg
    15841 gcggaggcac tggcagacct gcctgccctt taccgtgaaa tggatgattc ccgcctgatg
    15901 acgctgctca gtgatgccat gtttgctgcg gaaatgaagg ggatgctgga tggcacaggg
    15961 gattgattta ggttatgccg ccacgcttcc ctcaaaagag gcggtggcat acttccgcgc
    16021 caaaggggcg catatcagct ggaactggtt cgaaacagac gcggatgttc atgcccgttc
    16081 attcacggcg gcaaaagtgg cacgcctgga cgtactgacc acactacagg cggaagtgca
    16141 gcgtgctgtt gatgagggga tttcacagaa agcatttatc cgcacactga caccccgcct
    16201 gcaaaagctg ggatggtggg gaaagcagat tgtggtggac agcgccggta acgcagaaga
    16261 agtgcaactg ggcagtcccc gccgtctggc gctgatttac aacgtgaaca cccgcgtggc
    16321 ttacaatgcc gggcgttaca cgcagatgat gaacaacacg gacacgcatc cgttctggca
    16381 gtatgtggcg gtgatggaca gccgcacccg cccggcgcat tccgccctta acgggcaggt
    16441 attccgctat gacgatccgt tctggaatac acactacccc cccaatggct ggaactgccg
    16501 ctgccgtgtc cggccattgt ctcagacccg tctggatgca atggggttat ccgtttcatc
    16561 cggtcaggat catctttcca cccgcaatgt tgaggctggc gtggataagc agaccggaga
    16621 agtcagagaa atgccggtga ccacatattc agatggcacc agaaccatga caccggatgt
    16681 gggctggtca tataaccccg gctcggcggc gttcggcaca gaccaggcgt tgatccgtaa
    16741 actgatcgag gtgaaaagcc cggcgctgcg ggaaatggta gtgcaggaga tgaacaacag
    16801 cccggagcgg cagctggcgt tccgcatctg ggcaaaaaac atcatgaaaa cccggcgagg
    16861 cggtaacgat atccgcacgc tgggctttat gaccgaaagc attgcgcagg cggtggaaag
    16921 ccggacggga acgccaccgg cccgcctgct ggcgatgagc ggtaaaaatg tgctccatgc
    16981 ggacagcatg aaacatcaga atgacggtat cgccctgacg ccggaggatt tcggtcgatt
    17041 accggcaatg ctggcaaagc caaaagcggt gctgtgggac aaacgccaca acaatctgat
    17101 gtatatcgtg gaaagtaagg atagcagtgt gcagattgcc gttaatgcgc cgtacagcct
    17161 gaagcgtcag ccggataaac tggatgtgat tgtgaatgct taccgggtta tcaatatgga
    17221 taagctgaaa tcggatatcc ggggtggaat gcttgaggta ctggaaggag atattgatta
    17281 acggtcagtg gcgggggttg aacccgcata catgatccac ccggaggcag gcaccgactt
    17341 taccagttaa gcgtacactg accgcctgtt tattttatat tcatccttta tgcggaggca
    17401 atatgtcatc cattgatgcc gctgtcgttg ttgacgttac gcgtctccag cgggtctttg
    17461 cccggcttca gtttgtgggt ggagggaaag acctggcccg cagtgtggcg gcgagtcttc
    17521 tgtcatcgtc agaaatggcg tttgaacagg aaaaagagcc ggacggcgaa cgctggcatg
    17581 actggtcaga tccttaccgc aagtggcgta cccgtaaggg atacatgccc ggcaaaatcc
    17641 tgacgctgaa cggcgatctg gcccgacgcc tgaccacgga ttatggcgat acctgggcgc
    17701 tgattggatc aaatgagcct tatgcggcca ttcatcagtg gggtggcctg cctggtatgc
    17761 caccgggacc ggcggccatt ggcgcacgcc cgtatatggg ctttgatcag gtggcagagc
    17821 aggagatcat ggacgaaatc agaaaacgct ttaaaaaggc cacagaaacg ccttaattgt
    17881 ttaaggtatg caaatgcatt accttacccc cttcaggcgc gtgtcgtgat tttctaacct
    17941 gtatttaacg ggctttaaaa tctgcgtgaa gcgcctttgt atttttcccc tggaagcact
    18001 tcctgaaatc ccgcaaaacc cgccctgaaa attcccggct tatgctgccg gaatgaagac
    18061 gaaaaacacg cccaaacttg cttatgccat tctgaatgcc atcagcctgt ccgcagacgg
    18121 ggacggtgac tggtgtcaga tcatgccagc cggtcgggta aaggcccgtg acggtcgccc
    18181 ggaaaaaccg gccgagggct ggctgattaa ccgtgcgacc gttgagcgca tggtatcccg
    18241 ggttgtggcg ctcaatcagc cggtgaaaat tgactacaac caccagaccc tgattaaggg
    18301 gcatcaggca ccggctgccg gttttgtgat ggcctcaccg gagaattttc gcttcagtga
    18361 agagcgtggc ttcgaggtgc gcccgaagtg gaaccctccg gcccttgaac atctgcgtaa
    18421 taacgaattc ccctggtttt caccggtgat tggctatgac gagaacaccg gcgaacctgt
    18481 cgaacttcgg atgctggcta ttaccggtga ccccggtctg accggcatga atcctgtcgc
    18541 cgcgctgtcg gcggatgacc tttataacgc cttaaaccct cctttaaagg atacctccat
    18601 gaatgagcaa ttacgccagt tgctgacggc gctcggtctg accgtggctg acggtgacga
    18661 atttacgccg gagctgggca cggcggcgct gtctgccctc accgggatca aaacccgtgc
    18721 ggatgcacac gacaacctga aaacacaggt cgccagtctg tcggcagagc tggaaaccgc
    18781 aaaaggcaca ccggctggcg gcattattga tctgacgaaa tacgtgcccg ttgaaacgta
    18841 taacgccctg cgtaccgaat acgttgcgct gtcggcgcag cacggcagca ccacgctgga
    18901 gcaggtgctg gacaaggctg aatccgaagg gcgcatcttc aaaagcgaac gcggctatct
    18961 ggaacaactg ggcgggcaga ttggcgttgc ggcactttct gcacagcttg acgcccgcca
    19021 gccggttgcg gccctgacct ctcttcagac cgacaccgtg accgtgccgg ataaaaagac
    19081 tgccaccgct gcgctgtcgg ctgaagatat cgcggccgcc aaactgctgg gtaaaaccga
    19141 agccgagttc ctgaaaatga aagaggaaat gcaataatgc ctaccccgat tacaccggcg
    19201 atgatcacag ccctgatgac gggctaccgt tctgattttc aggccgggat gtccatggcc
    19261 ccgtcgcagt ataaaaaaat tgcgatgacc gtgccctcca catcgaaatc caacacctac
    19321 ggctggctgg ggcagttccc gcagttccgt gagtggatcg gctcccgcgt catcgagaag
    19381 atgaaagcct atggctatgc catcgtgaac aaaacttttg aaggcactgt cgccattagc
    19441 cgcgatgact tcgaagacga caacctcggt atttattccc cgttgttcca ggagatgggg
    19501 cgcgctgcgg cggtacaacc ggatgagctg gtctttgctg ccctgcgtga cgggatcagt
    19561 gcggcctgct atgacggtca gaacttcttt gataccgaac atccggttta ccccaaagtg
    19621 gatggctccg gggatgcgca gatggtcagc aatatgtttg tggcaaaaac cggctctgtc
    19681 ggcgcacagg ctgattacag cggtccggcc tggtatctgc ttgactgctc ccgcgcggta
    19741 aaaccgctga tttatcagga tcgccgtaag gctgaactgg ttgcccagac caaagtcgat
    19801 gaaggtcgcg cattcaccga taacgaattt gtgtttggtg catccgcccg tcgcaatgtg
    19861 ggctacggct tctggcagat ggcctacatg atgcagtcac cgctgacgct ggatgcactg
    19921 tggcacggct ggtcagccat gcgcgaattt accgctgacg gtggccgcaa actcggcatt
    19981 aaacccaccc atattgttgt ccccacctcg ctggaaaaac aggcggtgca gctgctggag
    20041 cgtgaactgt tcgcagacgg aaacgcaacc gtctctaacg agatgaaggg taagctggag
    20101 ctggttgtcg cggattatct gtaaacggtg agccgggctt acggcccggc ccctgcggag
    20161 ggcaaatatg aatgaacatc acactgtggc agcaacagat gattcaggtc ttcaggtcag
    20221 tggtgacaat tctgtcacgg tgctggcaga agtgcgttgc agtcgttcgg cgtttcgtcg
    20281 tgcggggttc ctgttcacgc gtggacgtca gcaggttgag gtcaccccgg agcagcttgc
    20341 ccgactggag gaggagccat gcctcactgt gcgaatacta cagacgtctg ctgatgatgc
    20401 ggggagcgtg gcgggtgtgg ttcatgcagt ggccggtaca gatt
//
LOCUS       DAZFWP020000088        20289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFWP020000088 DAZFWP020000000
VERSION     DAZFWP020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.088"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(112..2181)
                     /gene="glyS"
                     /locus_tag="ACHK0Z_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             complement(112..2181)
                     /gene="glyS"
                     /locus_tag="ACHK0Z_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU4503040.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2191..3102)
                     /gene="glyQ"
                     /locus_tag="ACHK0Z_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             complement(2191..3102)
                     /gene="glyQ"
                     /locus_tag="ACHK0Z_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU4503041.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3197..3496)
                     /locus_tag="ACHK0Z_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             complement(3197..3496)
                     /locus_tag="ACHK0Z_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU4503042.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3671..4666
                     /gene="wecH"
                     /locus_tag="ACHK0Z_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             3671..4666
                     /gene="wecH"
                     /locus_tag="ACHK0Z_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU4503043.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4708..5145)
                     /gene="yiaA"
                     /locus_tag="ACHK0Z_RS15305"
                     /old_locus_tag="pgaptmp_003061"
     CDS             complement(4708..5145)
                     /gene="yiaA"
                     /locus_tag="ACHK0Z_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU4503044.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5191..5532)
                     /gene="yiaB"
                     /locus_tag="ACHK0Z_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             complement(5191..5532)
                     /gene="yiaB"
                     /locus_tag="ACHK0Z_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU4503045.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5701..7155)
                     /gene="xylB"
                     /locus_tag="ACHK0Z_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             complement(5701..7155)
                     /gene="xylB"
                     /locus_tag="ACHK0Z_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU4503046.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7227..8549)
                     /gene="xylA"
                     /locus_tag="ACHK0Z_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             complement(7227..8549)
                     /gene="xylA"
                     /locus_tag="ACHK0Z_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU4503047.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8915..9907
                     /gene="xylF"
                     /locus_tag="ACHK0Z_RS15325"
                     /old_locus_tag="pgaptmp_003065"
     CDS             8915..9907
                     /gene="xylF"
                     /locus_tag="ACHK0Z_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4503048.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9985..11526
                     /gene="xylG"
                     /locus_tag="ACHK0Z_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             9985..11526
                     /gene="xylG"
                     /locus_tag="ACHK0Z_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU4503049.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11504..12685
                     /gene="xylH"
                     /locus_tag="ACHK0Z_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             11504..12685
                     /gene="xylH"
                     /locus_tag="ACHK0Z_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU4503050.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12763..13941
                     /gene="xylR"
                     /locus_tag="ACHK0Z_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             12763..13941
                     /gene="xylR"
                     /locus_tag="ACHK0Z_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU4503051.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEIML
                     "
     gene            complement(14049..14873)
                     /locus_tag="ACHK0Z_RS15345"
                     /old_locus_tag="pgaptmp_003069"
     CDS             complement(14049..14873)
                     /locus_tag="ACHK0Z_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU4503052.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15193..17223
                     /gene="malS"
                     /locus_tag="ACHK0Z_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             15193..17223
                     /gene="malS"
                     /locus_tag="ACHK0Z_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4503053.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17401..18654
                     /gene="avtA"
                     /locus_tag="ACHK0Z_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             17401..18654
                     /gene="avtA"
                     /locus_tag="ACHK0Z_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU4503054.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18806..19279)
                     /locus_tag="ACHK0Z_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             complement(18806..19279)
                     /locus_tag="ACHK0Z_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4503055.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19381..20229)
                     /gene="yiaJ"
                     /locus_tag="ACHK0Z_RS15365"
                     /old_locus_tag="pgaptmp_003073"
     CDS             complement(19381..20229)
                     /gene="yiaJ"
                     /locus_tag="ACHK0Z_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGU4503056.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
BASE COUNT         5101 a         5169 c         4978 g         5041 t
ORIGIN      
        1 attaatatat gagataatct ttattcagca gaagattatt aaaggttgct gtattattta
       61 gcgataataa aaaaagcctg ccagatggca ggctttttaa taacggcgtt attactgcaa
      121 cagcgaaata tccgcaacgc gcaggaacag ttcgcgcagt ttctccagca tggtcagacg
      181 gttgagacgc aattctttgt catcaaccat gaccatcact ttatcgaaga aagcatcaac
      241 cggctcacgc agctctgcca gttcgaccag cgcatcctgg taacgacctt ccgcaaagta
      301 cggctccagc ttgtcacgta gcaccacaac ctgcatcgcc agtttaattt cttccggctc
      361 tttcagggta gaggcattca cgcggtcgct cagcacttcg tcagatttcg ccaaaatgtt
      421 ggagacacgc ttgttcgccg ccgccagtgc agcagctgct tcaagggtac ggaagtgcga
      481 taccgctttc atgcgggcat cgaaatcagc cggacgagtc ggacgacgcg ccagtaccgc
      541 ctggatggtg tcaacggtgt aaccttcgtc ctgataccag gcgcggaagc gaccgagcat
      601 aaagtcgata acatcatcaa ccacgttggc gttagtcagc ttatcgccat acagacgcac
      661 tgcttcttcg gtcagcgttt gcagatcaag gttgaggttc ttctcaacga taattcgcag
      721 cacgccaagt gcggcacgac gcagcgcaaa cgggtctttg tcgcctttcg gatgctggcc
      781 gataccgaag atacccgcca gagtatccat cttgtcagca atcgccagcg cacaggctac
      841 cgggttagac ggcaggtcat caccggcaaa gcgcggctga tactgctcat tcagcgccac
      901 cgcgacatct tccgcttcgc catcgtgacg cgcatagtgc atccccataa cgccctgggt
      961 gtcggtgaac tcgaagacca tgttggtcat caggtcacac ttagacagca ggcccgcacg
     1021 ggttgcgtgg ttaacgtcag cgccaatctg ttcagcaatc cagccagcca gcgcctggat
     1081 gcggtcagtt ttgtcgcgca gcgtacccag ctgttgctgg aacagcacgg tttgcaggcg
     1141 cggcaggtta tcttccagac gttttttacg gtcggtattg aagaagaact cggcatccgc
     1201 cagacgtgga cgaacgactt tctcgttacc agagataatt tgctgcggat ctttcgattc
     1261 gatgttggca acgaagatga agttcggcag cagtttgcca tcgttcgcat acaccgggaa
     1321 gtatttctgg tcacctttca tggtgtaaac cagggcttca gccggcaccg cgaggaattt
     1381 ctcttcgaat tttgcggtca gaacgaccgg ccactccacc agcgaagcca cttcttccag
     1441 caggctttcg cttaagtcag cgttaccgcc aatcttacgc gctgcttctt cggcatcggc
     1501 tttaatcttc gctttacgtt cttcgtaatc ggcgatgact ttcccgcgct cacgcagaat
     1561 ttccggatac tgatcggcgt tgtcgatggt gaattccggt tcgcccataa agcggtggcc
     1621 gcgaatcacg cgatcggact gaatgcccag aatggttgcc ggaatgactt tgtcgcccag
     1681 cagcagggtc acggtgtgca ccggacgcac gaagtgcacg tcgcttgcgc cccaacgcat
     1741 cagtttcggg atcggcagtt tcgccagaga agtcgcaacc atattcggca gcagtgcttc
     1801 ggtgctttcg cccttcacat gggcgcgata cagcagccat tcgcctttat cggtagtcag
     1861 acgctcagcc tggtcaacgg taataccgca accacgcgcc caaccttctg ccgctttgct
     1921 cggtttgcct tcagcgtcga acgcctgggc aatcgccggg ccgcgttttt cgatttcacg
     1981 atccggttgc gcttccgcca ggttagctac tttcagcgcc agacgacgcg gagcagcaaa
     2041 ccattgaacg gtgccgtgtg cgaggccagc gttatccagc tccgcagtaa aattcgcagc
     2101 aaaggactca gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc cgatttccac
     2161 cagaaaagtt ttctcagaca tagccgcctc ttacttatct ttgttgcaca tcgggaagcc
     2221 gagggcttca cgggaagcgt agtatgcttc tgccactgct ttggtcaggg tgcgaatgcg
     2281 cagaatgtag cgctgacgct cggtgacgga gatggcttta cgcgcatcca gcaggttgaa
     2341 gctgtgggcg gctttcagaa tacgctcgta ggctggcagc ggcagcggat tttccagcgc
     2401 cagcagctgc tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga agtccacatc
     2461 cgcgtattcg aagttgtaag tggactgctc cacttcgttc tgatggaaca cgtcgccgta
     2521 ggtggtttta cccagcgggc cgtcgctcca gaccaggtcg taaacgctgt ctacgccctg
     2581 aatgtacatg gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg gtttacactc
     2641 cagaccacca acctgctgga agtaagtgaa ctgcgtcact tccatgccgt ttagccacac
     2701 ttcccagccc agtccccagg cacccagagt cgggttttcc cagttatctt ccacgaaacg
     2761 gatgtcgtga atagtcgggt ccatgcccag ctctttcaga gaaccgaggt acagctcctg
     2821 aatattgtcc ggcgatggct taatgaccac ctggaactga tagtagtgct gtaaacggtt
     2881 ggggttttcg ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat aagcagccgc
     2941 catcggttct ggccccagcg cgcgcagaca ggtcattggg tgagaggttc ccgcgccgac
     3001 ttccatgtcc aatggttgaa caatggtgca gccctggcga gcccagtaat cctgtaaggt
     3061 caggatcaag ccctggaagg tcctggtatc aaacttttgc atattatttc gtgctggata
     3121 cgtgtggatt taaaggaagg gatcagtata cccgctggat ggaagatata cagtacgaaa
     3181 cgggaaaaag cagggcttaa cgcatggaaa gatgcaaaaa atggccatct gcgtcaaaag
     3241 aacagacaaa ctgctcttta cggaaggtat aaccgcgcat ttcatagctt ccctggaact
     3301 gttcgaaggc ggtgacgtcg attttttgct cgccggtgtt ataacgtttt gccgcctgat
     3361 ccttgcataa cgcttccata ttcaacgaac gcagaggatc aacctttacc cgttgtgctt
     3421 tctgcacggg tgacggtgta gaacaaccca ctagcaccat aagcgccatt gccggaaaga
     3481 aagcgttcat catcattaaa ttaccgtctg gtttgcaggt gagtcttatt atttatatcg
     3541 gtatagtggc tgatatctta cgaatttcct taagcctcta taagctgtag tgataaaact
     3601 cagattattc cagtgcgcca actgaatgat ttatcggtca gacttttttt aaccagatac
     3661 agagacacag atgcagccca aaatttactg gattgataac ctgcgaggga tagcgtgttt
     3721 aatggtggtg atgattcaca ccactacctg gtatgtgacc aatgctcata gtgttagccc
     3781 cgtcacctgg gatatcgcca atgttctgaa ttctgcctct cgtgtcagcg tgccgctatt
     3841 tttcatgatt tccggctatc tcttttttgg cgaacgcagc gcccagccgc gccatttctt
     3901 gcgtatcggc ttatgtctga ttttttatag cgcaatcgca ctgctctaca ttgcgctgtt
     3961 tacctccatc aatatggagt tagcgctgaa aaacctgctg caaaagccag tgttttacca
     4021 cttgtggttt ttcttcgcga ttgcggtgat ttatctggtt tcaccgctga ttcaggtgaa
     4081 gaacgtcggc ggaaaaatgt tgctggtgct aatggtggtg attggcatta tcgctaaccc
     4141 aaacacagtg ccgcagaaaa ttgacggttt tgaatggctg ccaattaact tatatatcaa
     4201 tggcgatact ttttactaca ttctgtatgg catgttgggc cgcgctatag ggatgatgga
     4261 cacacagcat aaagcactgt cgtgggtgag cgccgcgctg tttgcgacgg gggtttttat
     4321 tatctctcgc gggacattat atgaattgca gtggcgcgga aattttgccg atacctggta
     4381 tctttactgt gggccgatgg tttttatctg cgcaatcgcg ctattgactc tggttaaaaa
     4441 cacgctggat acgcgtacca ttcgcggact tggcttaatc tcccgccatt cattgggtat
     4501 atacggattc cacgccttga ttatccatgc gctgcgcacc cggggaattg agcttaaaaa
     4561 ttggccaata ctggatatta tttggatctt ttgcgcgacg ttggcagcga gtttgttact
     4621 ttctatgctg gtacaacgaa tcgacagaaa cagattagtg agttaagtaa aagcccgttc
     4681 acattggact gaccgggctt acgtgaatta ttcgctgtat tcttcctggg taacctgtgt
     4741 ttcttttggc gggtttattc cggtatcacg aatattcttc tgcaccgcta ctgcaccaaa
     4801 caagcttaag aagaaagcca gtccataaaa acctttttca ctgagtaata atgtcgcatt
     4861 ccacagacct accatcagta atgcaacaga gataataaag acagtcaggc aggtcatata
     4921 ataaatggaa gtggtcggta tgccttcata cttgtcccga acggtctttt gataagacgc
     4981 agcagaaaac agtcctaaca ccagtacggc aaaataatac cctttttcat ttaactgcat
     5041 ctctgcattc catagcccta acagataggt aacgatacca ccaacgagag ctatccatga
     5101 cacaatacta aaggccggtg aataggttga tattttgttg tccatcacag tattcctttc
     5161 attcctgaat gtgtaagagc tttatgttgc tcataccgag cgaaacaaac gcatttgacc
     5221 aaacaagcag acaaaaaagg cgatggggta gatcaccatt tcataagcat tgataggtgc
     5281 atttaaaaca ccgataaaca agagaccgat agtgattaac gcaactaact ggcagatatg
     5341 ggtaaaaaaa ttatccagtt gcccgagttt ttctgcgcga agatatgcat agttaccaaa
     5401 agttgctgtc attaacacgc caagaaaata gccttttcca cttagcaatg ggcatgagat
     5461 ccatagccca accagataaa ccagcgcccc gacaacaaat aatagttttg ccacagtttt
     5521 tgaggttttc ataacgatct ccatatctac cagcgataca ttacgagtaa ccaacgaaag
     5581 acaaaactga aaaatgccat taacaaatga ttttcagaat aaattcatac taaatattaa
     5641 ttaattagtg agatatatag atgtgaatta tcccccaccc ggtcaggcag gggataacgt
     5701 ttacgccatt aatggcagaa gttgctgata gaggcgacgg aacgtttcac gtcgtggctg
     5761 ataagcggca taacgctgcg catctggtag atgcgactgt tctaacggta gctgcggcaa
     5821 caactcaatg agcgatttct ctggattcgc cgcaatctgc gccagccttg ctgcgcccag
     5881 tgctggcccc acatcccccc ccgtacggta atcgagctgc tgaccgctga tatccgccag
     5941 catctgacgc cagtactcac tacgcgcccc gccaccaatc aacgtaacac tttgcggttt
     6001 aatgccgcag gcatgcacga catccatgcc atctgccagc gcataaccca cgccttccag
     6061 cactgctcgc gccagttcat tggggccatg ttgatgagtc aaaccaaaga aaaccccctt
     6121 cgcctgggga ttattgtgtg gtgtacgctc gccggaaaga taaggcagaa accaaactgg
     6181 ctcggcactt tcatcagcct gttgagctgc agcgattaaa gctgggacat tgcacaggcc
     6241 ggttaatttc gcgacccaat ccagacacga tgctgcactc agcattacag acattaaatg
     6301 ccaacgctgc ggtagcgcat ggcaaaagct gtgtacagcg ctttctggct tgcttaagaa
     6361 cccttcgctg acagcaaaat agacccccga cgtccccagc gataacattg cctgattagc
     6421 atcaaccatt cccacaccaa ctgcaccagc tgcattgtcg ccaccgcctg cgacaactgg
     6481 caccgtcgcc ataccccacg ctttcgcaac ttcaggtaac aaagcaccag taatttcgct
     6541 gccttcatat aatgcgggca tctggtcacg agataagtcg caagcctgca gcatgacgtc
     6601 actccagtca cgctttgcga catccagcca catggtgcca gctgcgtcag acatatcgct
     6661 ggcaaactcc cccgtcatac gcagacgcaa gtaatctttc ggtaataata ctttgtcgat
     6721 ttgacggaat atctccggct catgccgctg aacccatagc aatttaggcg cagtaaatcc
     6781 gggcatcatc aggttgccgg taatcactcg tgattgcgga actctcgctt ccagcaaagt
     6841 gcactcttgc gcacagcgcc cgtcgttcca caaaatggca gggcgcaata cccgttgttg
     6901 agcatccagt aaggttgctc catgcatctg gccggcaata cccaatgctt taacgtcctg
     6961 cagagaatgc tgatcgccca gagctttcat tgcgcgatca gttgcctgcc accactgttc
     7021 cgggtcttgt tccgaccaga gtggatgcgg gcgcgaaacg gtcagctttt ccgtttgcga
     7081 agcaaccacc tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc
     7141 tatcccgata tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc
     7201 gggccaacgg actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt
     7261 tccagttgct cctggcgacc actctgatgc accggagaca aattatgttc ctgagcatat
     7321 ttggctaaat ctgccagtga catttggcct ttcaggattt gctggcccaa ttcgctattc
     7381 cagccggaat aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgcgct
     7441 gcaattttca gcgccagtgc catcgtatcc atcgcgccga tatgaccgta aaacagatca
     7501 tatttatcag tactttgacg acgtactttg gcatcgaagt tcagaccacc ggtggtgaaa
     7561 ccgcctgctt tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac
     7621 tggtcggtgt cccagcccag ttgcgcatcg ccacggttgg cgtcgacaga accgaacagg
     7681 ccaagcgcaa tggcggtggc aatttcatga tggaaagagt gacctgccag cgtcgcgtgg
     7741 ttagcttcaa tgttcagttt aatctctttt tccagaccaa actgtttcag gaagccatag
     7801 accgtcgcgg catcgtaatc atattgatgt ttggtcggtt cttgcggttt cggttcgata
     7861 agcaacgtgc cctggaaacc gattttatgt ttatgctcaa ccaccatctg cataaagcgg
     7921 cccagctgtt cacgctcctg acgcaagtcg gtatttaaca gcgtttcgta accttcacga
     7981 ccgccccaca ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca
     8041 acttgcgttg ccgcccaact gaagacttca ggatctgggt tcgtcgccgc acccgcgcca
     8101 tagcgagggt ttgtaaagca gttagcggtt ccccacagca gcttcacgcc gctctcttct
     8161 tgcttgcctg ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac
     8221 gcgccctcag gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg
     8281 aaaaactcaa atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc
     8341 tgccacggac gattaaacgc ccccacacca aacatgtccg ccccgttcca gcagaatgtg
     8401 tgccagtagc aggcggcaaa acgcaagtgc tcttccatac gcttgcccaa caccagttcg
     8461 tcgggattgt agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg
     8521 cgatcgagct ggtcaaaata ggcttgcata ttgaactcca taatcaggta atgccgcggg
     8581 tgatggatga tgtcgtaata ttgggcactc cctttcagtt gctcaattat gttatttcac
     8641 actgctattg agataattca caagtgtgcg ctcgctcgca aaataaaatg gaatgatgaa
     8701 actgggtaat tcctcgaaga gaaaaatgca ataagtacaa ttgcgcaaca aaagtaagat
     8761 ctcagtcata aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt
     8821 gttttcccct gtttagttgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt
     8881 aaagctgtcc tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac
     8941 cctttgcacc tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg
     9001 tatggcgatt gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa
     9061 aaaggcagaa tctctcggcg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac
     9121 acaaatgtcg cagattgaaa acatgataaa ccggggtgtc gatgttcttg tcattattcc
     9181 gtataacggt caggtattaa gtaacgttgt aaaagaagcc aaacaagaag gtattaaagt
     9241 attagcttac gatcgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa
     9301 cgaaaaagtc ggtgaactgc aggcaaaagc cctggtcgat attgttccgc aaggtaatta
     9361 cttcctgatg ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat
     9421 gaaagtgtta aaaccttacg ttgattccgg aaaaattaaa gtcgttggtg accaatgggt
     9481 tgatggctgg ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa
     9541 taacaaaatt gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc
     9601 attaagcgcg caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc
     9661 aggtattaaa cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacgtt
     9721 gttggcaaat actgccgcag aaattgccgt tgagttgggc aatggtcagg aaccaaaagc
     9781 agataccaca ctgaataatg gcctgaaaga tgtgccctcc cgcctcctga caccgatcga
     9841 tgtgaataaa aacaacatca aagacacggt agttaaagac ggattccaca aagagagcga
     9901 gctgtaagcg ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa
     9961 tgaattggct taggtggagt cgttatgcct tatctacttg aaatgaagaa tattaccaaa
    10021 accttcggca gtgtgaaggc gattgataac gtctgcttgc ggttgaatgc tggcgaaatc
    10081 gtctcacttt gtggggaaaa tgggtctggt aaatcaacac tgatgaaagt gctgtgtggt
    10141 atttatcccc atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg
    10201 agtcacatcc gcgataccga acgcaaaggt atcgccatca ttcaccagga attggccctg
    10261 gtgaaagaat tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc
    10321 attatggatt atgacctgat gacgctacgc tgtcagaagc tgctcgcaca ggtcagttta
    10381 tccatttcac ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa
    10441 attgccaagg cacttaataa acaggtgcgc ttgttaattc tcgatgaacc gacagcctca
    10501 ttaactgagc aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt
    10561 atcgcctgta tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt
    10621 tgcgttattc gcgacggaca gcacattggt acgcgtgatg ctgccggaat gagtgaagac
    10681 gatattatca ccatgatggt cgggcgagag ttaaccgcgc tttaccctaa tgaaccacat
    10741 accaccggag atgaaatatt acgtattgaa catctgacgg catggcatcc ggtcaatcgt
    10801 catattaaac gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt
    10861 gccggactcg ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc
    10921 ggacaatggg aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag
    10981 caagccatcg cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt
    11041 ccggtaatgg cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc
    11101 attagccagc ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat tcagcaactc
    11161 aaagttaaaa cgtcgtcccc cgaccttgct attggacgtt tgagcggcgg caatcagcaa
    11221 aaagcgatcc tcgctcgctg tctgttactt aacccgcgca tcctcattct tgatgaacct
    11281 accaggggta tcgatattgg cgcgaaatac gagatctaca aattaattaa ccaactcgtc
    11341 cagcagggta ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc
    11401 gatcgggtac tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg
    11461 actcaggagc aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc
    11521 gtctgaagtg aaattggccg taccgacatc cggtggcttt tccgggctga aatcactgaa
    11581 tttgcaggtc ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac
    11641 caccgatggt gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc agaccgcgat
    11701 taccggcatc ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc
    11761 cgtcggctca atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg
    11821 ctggcctttg ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcctg
    11881 gaacggatgg tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat
    11941 gttggcattt cgcggcatac tcattggcat caccaacggc acgactgttt cccccaccag
    12001 cgccgcgatg tcacaaattg ggcaaagcta tctacctgcc agcactggct tcatcattgg
    12061 cgcgcttggc ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc
    12121 tttgggtttg cagtctccgg cctctaccgc agtagtcggt cgccaggctt taaccgctat
    12181 catcgtatta ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct
    12241 gctgctgacg ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg
    12301 acgcatttat gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga
    12361 acgcaccaaa cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat
    12421 ccttagttct cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc
    12481 aattgcagca tgcgtgattg gcggcaccag cctggctggc ggtgtgggaa gcgttgccgg
    12541 agcagtaatg ggggcattta tcatggcttc actggataac ggcatgagta tgatggatgt
    12601 accgaccttc tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga
    12661 ctccgcaacc aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggttacgtat
    12721 taccggtttt gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac
    12781 cgcatcacat tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta
    12841 ggggaatatt tacaggcatc acaatcagaa tgggatattt tcattgaaga agatttccgc
    12901 gcccgcattg ataaaatcaa ggactggtta ggagatggcg tcattgccga cttcgacgac
    12961 aaacagatcg agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg
    13021 tatcaccttg cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg
    13081 gttgaaagcg catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt
    13141 cttccggaat caagcggcaa acgttgggcc actgaacgcg aatatgcatt tcgtcagctt
    13201 gtcgccgaag aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac
    13261 tggcaacatg cgcaaaatcg gctggcagac tggctacaaa cgctgccacc gcaaaccggg
    13321 attattgccg ttactgacgc ccgggcgcgg catattctgc aagtatgtga acatctacat
    13381 attcccgtac cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat
    13441 ctgtcgcgtg tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg
    13501 gcaaaactgt tgcatcgatt attagataaa gaagaaatgc cgctacagcg aattttggtc
    13561 ccaccagttc gcgtcattga acggcgctca acagattatc gctcgctgac cgatcccgcc
    13621 gttattcagg ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag
    13681 gtactggatg cggtcgggat ctcgcgctcc aatcttgaga agcgttttaa agaagaggtg
    13741 ggtgaaacca tccatgccat gattcatgcc gagaagctgg agaaagcgcg cagtctgctg
    13801 atttcaacca ccttgtcgat caatgagata tcgcaaatgt gcggttatcc atcgctgcaa
    13861 tatttctact ctgtttttaa aaaagcatat gacacaacgc caaaagagta tcgcgatgta
    13921 aatagcgaga tcatgttgta gcgatgatga gaattgtcgg cgtcacatca gggaactcag
    13981 cacagccacc tgatgcgagc aaaagcctta tccgaccgac aaatcagata aggctcaagg
    14041 aaatgcaatt acatatgcgc cgcgattaac cgttggttat cctggtacat tgcgaacagg
    14101 tagttattat aactcttccc tttggtcgag tagcccttca gcttgtgaat catcgctgtg
    14161 gcagtcactt cctgatccgc tttacgcagc tgcgcacgcg atttacggaa cgaagagtaa
    14221 gccgggtgcg tattcaggtt agtgacatag gcgctcaccg attctttgac agaactaaac
    14281 tgtgagtacc ctttcacttt acccggcgca ttggtacaac gtcctttcat gcatttcatg
    14341 ccgaacagat tgttattgtt gcgcgccagc ttcgacgttc cccaaccgct ttctgctgca
    14401 gccatcgtcg ccaccatact ggtagggata atgtctacgc gttcaagcaa ggtattccac
    14461 gggatttttc gcgtattacc agaccacttc accttgtagc gtttggcgat gtctttcaga
    14521 cgcgcacgct cagcaggtga ccattggccc tgatactgtt ttgaaatcag ccagttacgt
    14581 tctgcagtaa tggctgcatt ttggctggta atgtaaggca ttacggtccg gagaaacgcc
    14641 tttttccttg gtgttccgga agggtatttt cgcaaatcag gaagtgaact actctttgca
    14701 ctattgcgag aatactcttg tttactgctt acctgtttat tactagcttt agttaagtgg
    14761 gacttttgac tcgctgttgt tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga
    14821 gtgagtaaca taagaatcat cgccccatat cgtcgtatgg gagtcaaaat catcaggtct
    14881 cctggtcgga tttaatcatt ccaacacctt atatttttca caaatttgag agttgaatct
    14941 caaatcatat caaaaatagc tgtcaagagc accccaagga atagtccaaa tctgaaacta
    15001 tgtcacgtgt taacgattca gattggcgct aaatcgcaga aaatgtgggg gttatcgcaa
    15061 aattcagccg ttttttgcgc gagatcgctc acccttgctt ctcatcctgt ggacttaccg
    15121 ctcagggatg agttttgtct ggcttatcgc tggcaaactg tctgaaatcg cagcaataag
    15181 gactcatcca ccatgaaact cgccgcctgt tttctgacac tccttcctgg cttcgccgtt
    15241 gccgccagct ggacttctcc ggggttccct gcctttagcg aacagggaac gggaacattt
    15301 gtcagccacg cgcagttgcc caaaggtacg cgtccactca cgctaaattt tgaccaacag
    15361 tgctggcagc ctgcagatgc gataaaactc aatcagatgc tttccctgca accttgtagc
    15421 aacacgccgc ctcaatggcg attgttcaga gacggcaaat atacgctgca aatagacacc
    15481 cgctccggta cgccaacatt gatgatttcc atccagaacg ccgccgaacc ggtagcaaac
    15541 ctggtccgtg aatgcccgaa atgggatgga ttaccgctca cgctggatgt cagcgccact
    15601 ttcccggaag gagccgccgt acgggattat tacagccagc aaattgcgat agtgaagaac
    15661 ggtcaaataa cgttacaacc cgctgctacc agcaacggtt tactcctgct ggaacgggca
    15721 gaaactgacg cccctgcccc tttcgactgg cataacgcca cggtttactt tgtgctgaca
    15781 gatcgtttcg aaaacggcga tcccagtaat gaccagagtt acggacgtca taaagacggt
    15841 atggcggaaa ttggcacttt tcacggcggc gatttacgcg gcctgaccaa caaactggat
    15901 tacctccagc agttgggcgt taatgcttta tggataagcg ccccatttga gcaaattcac
    15961 ggctgggtcg gcggcggtac aaaaggcgat ttcccgcatt atgcctacca cggttattac
    16021 acacaggact ggacgaatct tgatgccaat atgggcaacg aagccgatct acggacgctg
    16081 gttgatagcg cacatcagcg cggtattcgt attctctttg atgtcgtgat gaaccacacc
    16141 ggctatgcca cgctggcgga tatgcaggag tatcagtttg gcgcgttata tctttctggt
    16201 gacgaggtga aaaaaacgct gggtgaacgc tggagcgact ggaaacctgc cgccgggcaa
    16261 acctggcata gctttaacga ttacattaat ttcagcgaca aaacaggctg ggataaatgg
    16321 tggggaaaaa actggatcag aacggatatc ggcgattacg acaatcctgg attcgacgat
    16381 ctcactatgt cgctggcctt tttgccggat atcaaaaccg aatcaactac cgcttctggt
    16441 ctgccggtgt tctataaaaa caaaacggat actcacgcta aagtcatcga aggctttaca
    16501 cctcgcgatt acttaaccca ctggttaagt cagtgggtcc gcgactatgg gattgatggt
    16561 tttcgggtcg ataccgccaa acatgttgag ttgcccgcct ggcagcaact gaaaaccgaa
    16621 gccagcgccg cgcttcgcga atggaaaaaa gctaaccccg acaaagcatt agatgacaaa
    16681 cctttctgga tgaccggtga agcctggggc cacggcgtga tgcaaagtga ctactatcgc
    16741 cacggcttcg atgcgatgat caatttcgat tatcaggagc aggcggcgaa agcagtcgat
    16801 tgtctggcgc agatggatac gacctggcag caaatggcgg agaaattgca gggtttcaac
    16861 gtgttgagct acctctcgtc gcatgatacc cgtctgttcc gtgaaggggg cgacaaagca
    16921 gcagagttat tactattagc gccaggcgcg gtacaaatct tttatggcga tgaatcctcg
    16981 cgtccgttcg gtcctacagg ttctgatccg ctgcaaggta cacgttcgga tatgaactgg
    17041 caggatgtta gcggtaaatc tgccgccaac gtcgcgcact ggcagaaaat cagccagttc
    17101 cgcgcccgcc atcccgcaat tggcgcgggc aaacaaacga cactttcgct gaagcagggc
    17161 tacggctttg ttcgtgagca tggcgacgat aaagtgctgg tcatctgggc tgggcaacag
    17221 tgacttttcc ggcttcccgt tcgtcagtac ctcgggaagc cgccaaccag gataaaatgt
    17281 cagccctaat cagcgttgca ggataaagca ccgctcactc tccaacagac cgatttgcac
    17341 cccagcaaat gtagcgttat tgttaccttc cttgctacag agttcgacag atatcccgct
    17401 atgacattct ccctttttgg tgacaaattt acccgccact ccggcattac gctgctgatg
    17461 gaagatctga acgacggttt acgcacgcct ggcgcgatta tgctcggtgg cggtaatccg
    17521 gcgcagatcc cggaaatgca ggactacttc cagacgctac tgaccgacat gctggaaagt
    17581 ggcaaagcga ctgatgcact gtgcaattac gacggtccac aggggaaaac ggagctactc
    17641 acactgcttg ccggaatgct gcgcgagaag ttgggttggg atatcgaagc acagaatatt
    17701 gcactaacaa acggcagcca gagcgcgttt ttctacttat ttaatctgtt tgccggacgc
    17761 cgtgccgatg gtcgggtcaa aaaagtgctg ttcccgctgg caccggaata cattggctac
    17821 gctgacgccg gactggaaga agatctgttt gtctctgcgc gtccgaatat tgaactgctg
    17881 ccggaaggcc agtttaaata ccacgtcgat tttgagcatc tgcatattgg cgaagaaacc
    17941 gggatgattt gcgtctcccg gccgacgaat ccaacaggca atgtgattac tgacgaagag
    18001 ttgctgaagc ttgacgcgct ggcgaatcaa cacggcattc cgctggtgat tgataacgct
    18061 tatggcgtcc cgttcccggg tatcatcttc agtgaagcgc gcccgctatg gaatccgaat
    18121 atcgtgctgt gcatgagtct ttccaagctg gggctgcctg gctcccgctg cggcattatc
    18181 atcgccaatg aaaaaatcat cactgccatc accaatatga acggcattat cagcctggca
    18241 cctggcggta ttggtccggc gatgatgtgt gaaatgatta agcgtaacga tctgctgcgc
    18301 ctgtctgaaa cagtcatcaa accgttttac taccagcgtg ttcaggaaac tatcgccatc
    18361 attcgccgct atttaccgga agatcgctgc ctaattcata aaccagaagg agccattttc
    18421 ctctggcttt ggtttaagga tttgcccatt acgaccgagc agctctatca gcgcctgaaa
    18481 gcacgcggcg tgctgatggt gccggggcac aacttcttcc cagggctgga caaaccgtgg
    18541 ccgcatacgc atcaatgtat gcgcatgaac tacgtaccag agccggagaa aattgaggcg
    18601 ggggtgaaga ttctggcgga agagatagaa agagcctggg ctgaaagtca ctaaaaattg
    18661 cctgatgcgc tacgcttatt aggcctacat gatttctgca atatattgaa tttgcaagaa
    18721 tttgtgggcc ggataaggcg tttacgccgc atctggcata aacaaagcgc actattccgg
    18781 cgttgagaaa cgccggaaaa acgtttcaaa caggctgctg ccgtagccga tgccgctgca
    18841 ctttctcgac atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca caggccggcc
    18901 ccgtctcccg atgccagcac agatcgcact taatcgccct cgcctttttg cgcgaagaaa
    18961 cgacctccat cgcaccaaac gggcaagcca gcatacagct tttacagcca atgcaacgtg
    19021 tttgttcaac gaaaatatgc ccatgttcgc ggcttatcgc gttaacaggg cagacattcg
    19081 cgcacggtgc atcttcacac tgatgacagg ctactgccgt ggtccagctg tggtctttaa
    19141 tgacacgaat acgggaaata aactcgtctg gtgacaacgc agcgcaatcc tgattctcct
    19201 gatgcgacac tgcgcaagcc acttcacagg tacggcaacc gatacatttc gtcgcatccg
    19261 caataataaa ccggttcatg atcactcctt tgcactaaaa cagcagagtg ccagagagcg
    19321 cgtccctact gctttgatcc aacgaaggaa atgttaaagt tttggcactt gtccaaagcg
    19381 ttatgtgatc gcgccctggt cgtccctgac agtaaatccc agttcattag aaatagcctg
    19441 cgcggtttca cgcagtggtt tcaggagatt tttctccccc acctgtttca gacgtgatgt
    19501 cgaaagcgaa atcgacacgg cgtacggcac ccgcccatga atattaaaca ccggaacagc
    19561 aatacaggag acgccgagtt cgttttcttc tctgtccatc gccgctccgc tttcacgaat
    19621 gtgcgccagt tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg ttaacggctg
    19681 gatctcatgc tgatggtttt cccagtatga cttcacgtag tccgggtgac caaacgccat
    19741 gtagatcttg cccattgcgg aacagtagag cggcatatgc tggccaatat aggcacgggt
    19801 tcgcagcatc ccggttgtgg gttccagctt ataaatcaaa atagcgtgat cgtcttcgcg
    19861 gctggagaag ttaatggttt caccagtggc gatgttcagt gcctcaagat gcggagcggc
    19921 gatatgaatg atattcagcg aagacagcgc cttctgcccg acggcaataa atttggtagt
    19981 caggcggtaa ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact gtaatccctg
    20041 caataagcga tggacggtac tcttatttaa accagccagc tccgaaagat gcgccaacgg
    20101 acaaccgttt ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa acagactctg
    20161 gcttccggct ggacgctctt tttcctgcgc catctcgttc tctttttttc ccatcacttc
    20221 tttccccatt ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc acgatctcga
    20281 cagataatt
//
LOCUS       DAZFWP020000089        20110 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFWP020000089 DAZFWP020000000
VERSION     DAZFWP020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.089"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            23..1477
                     /gene="ybbW"
                     /locus_tag="ACHK0Z_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             23..1477
                     /gene="ybbW"
                     /locus_tag="ACHK0Z_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU4503057.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1537..2838
                     /gene="allB"
                     /locus_tag="ACHK0Z_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             1537..2838
                     /gene="allB"
                     /locus_tag="ACHK0Z_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HGU4503058.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGEEAKREGRVTAHDYVASRPVFTEVEAIRRVLYLAKVAGCRL
                     HVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTDQFEEIGTLAKCSPPIRDLEN
                     QKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGGIAGLQNCMDVMFDEAVQKRG
                     MSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEYRHKVSP
                     YVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ"
     gene            2895..4196
                     /gene="ybbY"
                     /locus_tag="ACHK0Z_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             2895..4196
                     /gene="ybbY"
                     /locus_tag="ACHK0Z_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGU4503059.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4218..5363
                     /gene="glxK"
                     /locus_tag="ACHK0Z_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             4218..5363
                     /gene="glxK"
                     /locus_tag="ACHK0Z_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU4503060.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5492..6277)
                     /gene="allE"
                     /locus_tag="ACHK0Z_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             complement(5492..6277)
                     /gene="allE"
                     /locus_tag="ACHK0Z_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU4503061.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6288..7523)
                     /gene="allC"
                     /locus_tag="ACHK0Z_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             complement(6288..7523)
                     /gene="allC"
                     /locus_tag="ACHK0Z_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU4503062.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDICIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYWVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7545..8594)
                     /gene="allD"
                     /locus_tag="ACHK0Z_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             complement(7545..8594)
                     /gene="allD"
                     /locus_tag="ACHK0Z_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU4503063.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8911..10578
                     /gene="fdrA"
                     /locus_tag="ACHK0Z_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             8911..10578
                     /gene="fdrA"
                     /locus_tag="ACHK0Z_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4503064.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEMLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVACDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10588..11847
                     /gene="ylbE"
                     /locus_tag="ACHK0Z_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             10588..11847
                     /gene="ylbE"
                     /locus_tag="ACHK0Z_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4503065.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11858..12673
                     /gene="ylbF"
                     /locus_tag="ACHK0Z_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             11858..12673
                     /gene="ylbF"
                     /locus_tag="ACHK0Z_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4503066.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGTP"
     gene            12670..13563
                     /gene="ybcF"
                     /locus_tag="ACHK0Z_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     CDS             12670..13563
                     /gene="ybcF"
                     /locus_tag="ACHK0Z_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4503067.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKAYGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13700..14767)
                     /gene="purK"
                     /locus_tag="ACHK0Z_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     CDS             complement(13700..14767)
                     /gene="purK"
                     /locus_tag="ACHK0Z_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU4503068.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(14764..15273)
                     /gene="purE"
                     /locus_tag="ACHK0Z_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             complement(14764..15273)
                     /gene="purE"
                     /locus_tag="ACHK0Z_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU4503069.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15391..16113)
                     /gene="lpxH"
                     /locus_tag="ACHK0Z_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             complement(15391..16113)
                     /gene="lpxH"
                     /locus_tag="ACHK0Z_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU4503070.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKAEALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16116..16610)
                     /gene="ppiB"
                     /locus_tag="ACHK0Z_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             complement(16116..16610)
                     /gene="ppiB"
                     /locus_tag="ACHK0Z_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU4503071.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16784..18169
                     /gene="cysS"
                     /locus_tag="ACHK0Z_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             16784..18169
                     /gene="cysS"
                     /locus_tag="ACHK0Z_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU4503072.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18205..18726)
                     /gene="ybcI"
                     /locus_tag="ACHK0Z_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             complement(18205..18726)
                     /gene="ybcI"
                     /locus_tag="ACHK0Z_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4503073.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(18834..19046)
                     /gene="ybcJ"
                     /locus_tag="ACHK0Z_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             complement(18834..19046)
                     /gene="ybcJ"
                     /locus_tag="ACHK0Z_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU4503074.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19048..19914)
                     /gene="folD"
                     /locus_tag="ACHK0Z_RS15460"
                     /old_locus_tag="pgaptmp_003092"
     CDS             complement(19048..19914)
                     /gene="folD"
                     /locus_tag="ACHK0Z_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU4503075.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         4590 a         5117 c         5530 g         4873 t
ORIGIN      
        1 tacgtgaaat taagagggat ttatggaaca tcagagaaaa ctattccagc aacgcggcta
       61 tagcgaagat ctattgccga aaacgcaaag ccagcgaacc tggaaaacat ttaactattt
      121 taccttatgg atgggttcgg ttcataacgt tcccaattat gtgatggtcg gcggcttttt
      181 tattctcggc ttgtcaacct ttagtattat gctggcaatt atcctcagcg cctttttcat
      241 tgccgcggta atggtattaa acggcgctgc gggcagtaaa tacggcgtac cgtttgccat
      301 gatcctgcgt gcttcttatg gcgtacgtgg cgcactgttt cccggattat taagaggcgg
      361 tattgctgcc attatgtggt ttggcctgca atgttatgcg gggtcactgg cctgcttgat
      421 tctgattggc aaaatctggc cgggattttt aactctcggt ggtgatttca cgctgttagg
      481 gctttctcta ccgggcttaa ttactttctt actcttctgg ctggtcaacg ttggtatagg
      541 ttttggcggt ggcaaagttt taaataaatt cactgccatt cttaacccgt gcatctatat
      601 cgttttcggc ggtatggcga tttgggcgat ttcgctggtc gggctcggtc caatctttga
      661 ctacattccg agcggtattc agaaagcaga aaacagtgga ttcttgttcc tggtggtgat
      721 taacgcggta gttgcggtct gggcggcacc ggcggtgagc gcatccgact ttacgcaaaa
      781 cgcccactcg tttcgtgagc aagcgctggg gcaaacgctg ggtttagttg tggcctatat
      841 tctgtttgcg gtcgctgggg tatgtattat tgccggagcc agtattcact acggcgctga
      901 cacctggaac gtgctggata ttgttcagcg ttgggacagc ctgtttgcct cgttctttgc
      961 ggtactggtt attctgatga cgactatttc cactaacgcc accggtaata ttattccggc
     1021 cggttatcag attgctgcca ttgcaccgac aaaactgacc tataaaaacg gcgtactgat
     1081 tgccagtatt atcagcctgc tgatctgccc gtggaaatta atggaaaatc aggacagtat
     1141 ttatctgttc ctcgatatca tcggcggaat gctgggtccg gtaattggtg tcatgatggc
     1201 acattatttt gtggtgatgc gtgggcaaat taatcttgat gaactgtaca ccgcacctgg
     1261 cgattataaa tattacgata acggttttaa cctcaccgcg ttttcagtaa ctctggtggc
     1321 cgttatttta tctcttggcg gtaagtttat tccctttatg gaaccgttat cgcgtgtttc
     1381 atggtttgtc ggcgtcatcg tcgcctttgc ggcctacgcc ttattaaaga aacgtacaac
     1441 agcagaaaaa acaggagagc aaaaaaccat aggttaatta atcccgatat tgaacattga
     1501 gttaaaaacc aatctgtatt ttacaaggag tttgttatgt cttttgattt aatcattaaa
     1561 aacggcaccg ttattttaga aaacgaagct cgcgttgtag atatcgccgt taaaggcggg
     1621 aaaattgctg ctatcggtca ggatctgggc gatgcaaaag aagttatgga tgcgtctggt
     1681 ctggtggttt cgccgggcat ggttgatgcg cacacccata tttctgaacc gggtcgtagc
     1741 cactgggaag gttatgaaac cggtactcgc gcagcggcaa aaggtggtat caccaccatg
     1801 atcgaaatgc cgctcaacca gctgcctgca acggttgacc gcgcttcaat tgaactgaag
     1861 ttcgatgccg ctaaaggcaa gctgactatc gatgcggcac aactcggtgg cctggtgtct
     1921 tacaacatcg accgtctgca tgagctggat gaagtgggcg ttgtcggctt caaatgcttc
     1981 gttgcgacct gtggcgatcg cggtatcgac aacgacttcc gtgatgtaaa cgactggcag
     2041 ttcttcaaag gtgcgcagaa gctgggcgaa gaagcgaaac gtgaaggtcg cgtaactgcc
     2101 catgactatg tggcttcgcg tccggtattt accgaagtgg aagcgattcg ccgcgtgctg
     2161 tatctggcga aagttgccgg ttgccgtctg cacgtttgcc atatcagcag cccggaaggc
     2221 gttgaagaag tgactcgtgc acgtcaggaa ggtcaggacg ttacttgtga atcctgcccg
     2281 cattactttg tactggatac cgatcagttc gaagaaatcg gcactctggc gaagtgttca
     2341 ccgccgatcc gcgatctgga aaaccagaaa ggcatgtggg aaaaactgtt taacggtgaa
     2401 atcgactgcc tggtttccga ccactcacca tgccctccgg aaatgaaagc cggcaacatc
     2461 atggaagcat ggggcggtat tgccggtctg caaaactgta tggacgtgat gttcgatgaa
     2521 gcggtacaga aacgcggaat gtctctgcca atgttcggca aattaatggc gactaacgca
     2581 gcagatattt tcggtctgca gcaaaaaggc cgtatcgccc caggaaaaga tgccgacttc
     2641 gtcttcattc agccgaatag cagctatgtt cttaccaatg acgatctgga atatcgccac
     2701 aaagtcagcc cgtatgttgg ccgtaccatt ggcgcgcgta tcacgaaaac catcttacgt
     2761 ggtgatgtga tttacgacat tgaacagggc ttccctgttg cgccgaaagg tcaatttatc
     2821 cttaaacatc agcagtaatc tggcccctgc aatgcccgtc cttgcggcgg gcattctccg
     2881 gttaaggtgt gtttatgttc aattttgcag tgagccgcga aagcctgtta tcaggatttc
     2941 agtggttttt ctttattttt tgcaacacgg ttgtggttcc tcctacgcta ctttctgctt
     3001 ttcagttgcc gcaaagtagc ctgcttacgc tcacgcaata tgcttttctt gctaccgcac
     3061 tggcctgctt cgctcaggcg ttctgcggtc atcgtcgcgc tattatggaa gggccgggtg
     3121 gcctgtggtg gggaaccatc cttactatca cccttggcga agcatcgcgc gggacaccga
     3181 tcaacgatat cgctaccagc ctggcggtgg ggattgcgct ctccggcgtg ctgacgatgt
     3241 tgattggttt tagcggatta ggccatcgcc tggcacggtt atttacgccg tcggtgatgg
     3301 tcttgtttat gttgatgctg ggcgcgcagc tgaccactat ctttttcaaa ggtatgctcg
     3361 ggctgccgtt tggcatagcc gacccaaatt ttaaaattca gttaccgccg ttcgcgctct
     3421 cggtggcggt gatgtgcctg gtactgggga tgattatctt cctgccgcaa cgttttgccc
     3481 gttatggcct gctggtcggc accataaccg gctggttgtt gtggtacttt tgctttcctt
     3541 cttcgcactc gctctccggt gagttgcact ggcagtggtt cccgctcggc agtggaggtg
     3601 ctttgtctcc gggaattatt ctgacggcgg tgattacagg tctggtgaat atcagcaata
     3661 cctacggtgc gattcggggc acggatgttt tttatccgca gcagggcgcg ggaaatacgc
     3721 gttatcgtcg cagctttgtg gcgaccggat ttatgacgct gataaccgta ccgctggcgg
     3781 taattccatt ttcaccgttt gtttcatcca ttggtttatt aacccagaca ggcgattaca
     3841 cgcggcgttc gtttatttat ggcagcgtta tttgcctgct ggtggcgctg gttccggcac
     3901 tcacgcgact gttttgcagt atccctttac ccgtgagtag cgcggtcatg ctggtttctt
     3961 atctgccttt actcttttcc gcgctggtgt ttagccagca aataacgttt accgctcgca
     4021 atatttatcg actcgcattg ccgttatttg tcggcatatt tttaatggca ttaccgcctg
     4081 tgtatctgca agaccttcca ttaacgcttc gtcctctgct cagtaacggc ttattggtcg
     4141 ggattttact ggctgttctt atggataacc ttattccgtg ggaacgtatc gaataatttg
     4201 ttgaaaaagg attgataatg aagattgtca ttgcgccaga ctcttttaaa gagagcttaa
     4261 gtgcagaaaa atgttgtcag gcaattaaag ccgggttttc gaccctcttt cccgatgcga
     4321 actatatctg tttgccgata gcggatggcg gcgaaggaac ggtggatgcg atggtcgccg
     4381 cgacgggcgg caacatcgtg acgcttgaag tctgcgggcc gatgggcgaa aaagtgaatg
     4441 ctttttatgg ccttaccggc gacgggaaaa cggcggtgat tgagatggcg gcagcaagtg
     4501 gcctgatgct ggtcgcgcct gaaaagcgta atccgttgct ggcctccagt tttggtacgg
     4561 gggagttaat tcgtcatgcg ctggataacg gcattcgcca tattattctc ggcattggcg
     4621 gcagtgcgac ggtcgacggt ggtatgggca tggcgcaggc gctcggtgtg cgtttccttg
     4681 atgccgacgg tcaggtgctg gcagctaacg gcggcaattt agcgcgcgtg gcaagcattg
     4741 agatggagga atgcgatccg cgtctggcga actgccatat tgaagtagca tgtgacgttg
     4801 ataacccgct ggtaggggca cgcggcgcgg cggcggtgtt tggcccgcaa aaaggggcaa
     4861 cgccggaaat ggtcgaagag ctggaacagg ggctgcaaaa ttacgcccgt gttttacaac
     4921 agcaaactga aatcaacgtc tgccagatgg cgggcggcgg cgctgcgggc ggtatgggta
     4981 ttgcggcggc ggtatttctc aatgcggata ttaaaccagg tattgagatt gtgttgaatg
     5041 cggtcaatct tgcgcaggca gtgcagggcg cagcactggt gattaccggg gaagggcgca
     5101 tcgactcgca aacggcaggc ggtaaagcgc cgctgggtgt ggcgtcggtg gcgaagcagt
     5161 ttaatgtccc ggtgattggg attgctggcg tattgggtga tggcgtggaa gtggtgcacc
     5221 agtacggcat tgatgcggta ttcagcattt tgccacgtct ggcaccttta gccgaagtgc
     5281 tcgccagcgg tgaaaccaat ctcttcaaca gcgcacgaaa tattgcctgc gccattaaaa
     5341 taggtcaggg aattaaaaac tgacccttac ctttaaagcg gatgcgattt atatcgcatc
     5401 tttttttacc tgaatttact acaaaaaaag ccacggtata aaccgtggca aaatccaaca
     5461 tagctaaaaa taatcaggcg agtggtatga cttaaatctc gacgtcgcgg ttacaatctt
     5521 tcgagtaaat atagctgaac gcttcaccac gccctacgcc ataaccagcc tgtaaagaat
     5581 aagcgcccat aaagatgtaa tcgccttttt tcaccgggat ccagttattg tcgaggttat
     5641 aaaccccctg accggaaaga atataggcac cgtgttcctg aacatgtgtt tcgatataac
     5701 cgtggctggc acctggtgca aaagagagga tatgcatgtt catatcaaaa cctaactctt
     5761 tgggcagaaa atccagcagg atcacatcat ccataccttc atagtgaatg cgttccagtt
     5821 cgctggcatt gccagaaacc agccacggtg catggccttc taccggaaca tagcggcgct
     5881 tatataaaaa gatttggctg tcttcggcct gggcgttaac aaacgtcatt aaggagcctg
     5941 gtgggcaata aagatagcca ccttcgctta aggcaaatgt tttgccttcg gctttggcag
     6001 tgatatttcc agagatcaca tacaggaacg tttcaatgcc ttcgccaccg aagccctgtt
     6061 ggttgccacc gttttgatgc agtgtgacca gataatcaac aaaagaggca cccagctttg
     6121 gcgtggagag gattgtcgcg tcacaatttt caaagcccgg aataatattt tttaccagac
     6181 catccggggt taacagtgcg aaattaccgt gtttaacaat cgcacggtta gccagtaaat
     6241 catcgcggta accggtgacg ttatttaaat atcccattta tgactcctta tttctgccag
     6301 gcaagttgat aaagcatgag tgccaacgtt ttgacccctt cggcaaggtc ggtaatattg
     6361 gtgcgttccg ccgggttatg gctgatcccg ttgatgctgg ggataaaaat catgcaggtt
     6421 ggtacgcgag gcgcgaaaat ttgcgcgtcg tgcccggcac cactgtgcat cacccagtaa
     6481 ttcagttttt cactttcaca caattctgtc agggtggcga ccaggtcctt attcatcggc
     6541 acgggttctt cgtccatcca taaatcgata tcaatacaaa tgtccatttc atcgcaaatc
     6601 gcccgcatgt cgttttctaa ctgttgggtg aaatcacgca gcacggcagc gtcggtatga
     6661 cgacaatcaa tggtgaacgt ggttttaccc ggcaccacat ttaccgtatt cgggcgcggc
     6721 tctactttgc caaaggtcag aaccagcgga tcgcccatct ttttcgcttt ttcgatcgac
     6781 tgatggcaaa tgcgactgaa agcgtaaacc gtatcacgac gataacccat cggcgtggtg
     6841 cctgcatggt ttgattcgcc gttcagcgtc accgtatagc gacgttgccc gacaattgca
     6901 ttcaccacgc caattgactg cccattactt tccagcacac agccctgttc gatatgcagt
     6961 tcgacaaagg ctttaatatc ctgacgcgga gttagcgggg cgttcggaag agtaaatccg
     7021 caagccttca ttgcatcgac aaaactattt cctttggcat cacagatatt ccgcacgtcg
     7081 tcaggattcg ccagcccaaa gatattttta ctgccccaga agacatacgg gaagcggctg
     7141 ccttcttctt ctgccatcgc caccacttcg accgtacgta gcggcgcgcc gtattgcgtt
     7201 ttcaaccagt caattgccag ccacgccgcc agcgcgccga attgcccgtc gaggttaccg
     7261 ccgttaacca cggtatcgat atgcgaaccg ctcagaatca cttcctgtgg atattcggta
     7321 ccattcaggc gaccgtataa attccctact tcatcgaaac gtgtttccag cccacttgct
     7381 gccattcttt ttttaaattg ctgctgggtt tccagccatt ccggcgaata aagtaaacgg
     7441 gtcatcccac ccgttgggtc agcgccaaaa gaggaaagcc agggcagcgt ttcttctatg
     7501 gcttgacgaa aatgtgtaat cataagaaag tcctgtctca ataattattg cgcaaaggga
     7561 tttttcgttt cgtatgacgt gttataaagc gcgtcggaaa ttaaatactg gtaaatatca
     7621 tcaacaattt cgatgccttc gacggcggct ttgcgttgtt taatatcctg atcctgtccg
     7681 ggataataaa cttggttaaa accgggcgcg ggggtaatgg catttaattc gcgcatggtc
     7741 tggctaagat gttgacggaa taattcgctg gaggagaaaa agttcggatt aataactata
     7801 tgtaattgcc ccaaattacg ccctgcgtgt aaatcgtcat acatcgaact aacctgtcgc
     7861 ccgaacggta agccgagtaa gacgcctgag aggacgtcaa tcatcatcat caggccatac
     7921 ccttttggcc cggcggcggg gagcagagca tgtaccgcga acggatcggt tgttggtgca
     7981 ccgtttttat cgaccgccca ggtatccggg atagacatat tacgcgagcg ggcgtcgagc
     8041 acttttcccc atgcctgtac ggtagtcgcc atatcaaagg taaggatctc gtcgccttct
     8101 cccggcgcgg caaaggccag ggggttagta ccgtagtaaa tttccgcgcc gccaaacggc
     8161 accaccattg gatcggactg gcacatcgaa atgccaatga atccggcgcg ggctgcctgc
     8221 tgcacaaaat aagagattgc gccgctgtga cccatccggc tgataccgac caccgcaacg
     8281 ccattttgct gggcggtttt gatggcatgt tccataccca ttttcgccgc gacctgtccg
     8341 gcggcattgt cggcatgtaa aattgccgag catggcccgg tttcctcaag acgaaactcc
     8401 ggttcgcggt tggtgccgcc ttttgaaatg cgttccgcgt agtattccac gcgcaccgcg
     8461 ccatgagagt ggatccctct ggcatcggcg taaaccaata cttcagccac ggttgcagcg
     8521 tgctcacgtt ttaacccagc ctggcagagt ttattctcaa ttagctggtg gagtgtttcc
     8581 cgactgattt tcatctgtct tcctttttaa cgacggtgtg aagcatgact gcaattaaca
     8641 tacagggaaa atatctggat tatgtgatcc agacaggcaa aaaaatatag ttagaattta
     8701 tttgataatc cgctcgcttt taacctggtt tttaaaacaa ccacgcttat taaaaaataa
     8761 cacgtaatag catggcggtt atttgaattc ttttgttaat aatccctgtg tgatattcat
     8821 caccttattt acccgttgtc atcgataccg taatcgccac attaacactg ctcgtgcaat
     8881 tgccatgggt gcaattttta aggagttgtt atgatccacg cctttattaa aaaagggtgt
     8941 tttcaggatt cggtcagttt aatgattatt tcacgaaaac tcagcgaatc agaaaatgtt
     9001 gatgatgttt ccgtaatgat gggtacgccc gccaataaag cgttattaga taccacaggt
     9061 ttctggcatg acgattttaa taacgccacg ccgaacgata tttgcgtggc aattcgtagc
     9121 gaagcggcgg atgcggggat cgcgcaggcg attatgcagc agcttgaaga ggcgctaaaa
     9181 caactggcgc aggggtcagg cagcagccag tccttgacgc aggtgcgtcg ctgggacagt
     9241 gcctgtcaga aattatccga tgccagtctg gcgctgattt cagtggctgg cgagtatgcg
     9301 gcggagctgg cgaatcaggc gctggatcgc aacctcaacg tgatgatgtt ctccgataac
     9361 gtcacgctgg aagatgaaat ccaacttaaa acccgcgcgc gagaaaaagg cttgctggtg
     9421 atggggccgg actgcggtac gtcgatgatt gccggcacgc cgctggcttt tgctaacgtg
     9481 atgccggaag gcaacattgg cgtcattggc gcttccggta ccgggattca ggagctgtgc
     9541 tcacagattg cgctggcagg ggagggaatt actcacgcga ttggccttgg cgggcgcgac
     9601 ctcagccgtg aagtgggcgg catcagtgcg ctgacggcgc tggaaatgct cagtgcagac
     9661 gagaaaagcg aaatgctggc atttgtttca aaaccgcctg ccgaagctgt gcgtctgaaa
     9721 attgttaatg ccatgaaagc aaccggcaaa ccgacggtgg cgctgttttt aggttatacc
     9781 ccggcggtgg cctgcgacga gaatgtctgg tttgcctcct cgctggatga ggccgcacgc
     9841 ctggcttgcc tgctttcacg cgttacggcg cgacgtaacg cgataacgcc tgccagcagc
     9901 ggatttattt gcggtttgta taccggcggt acgctggctg ccgaagcggc gggattactt
     9961 gccgggcacc ttggcgtgga agccgacgat acccatcatc acggcatgat gctcgacgcc
    10021 gatggtcacc agattattga cctcggcgat gatttctaca ccgtcgggcg tccccatccg
    10081 atgatcgacc cagccttacg caatcagtta attgccgatc tcggcgctaa accgcaagtg
    10141 cgcgtattgc tgcttgatgt cgtgattggc ttcggtgcga ccgccgatcc tgccgcctcg
    10201 ctggtaagcg cctggcaaaa agcctgtgcc gcgcgttcag acaatcaacc actgtatgcc
    10261 attgccacgg tgacaggcac tgaacgtgac ccgcaatgcc gctcgcagca aatcgccacg
    10321 ctggaagatg cagggatcgc ggtagtgagt tcgctgccgg aagccacctt gctggcggcg
    10381 gcactgattc gtccgctttc gcctgccact cagcaacaca caccgtcatt actggaaaac
    10441 gtcgccgtga ttaacatcgg attacgcagc tttgcgctgg agctacaaag cgccagcaaa
    10501 ccggttgtgc attaccaatg gtcgccagtc gccggcggca ataaaaaact ggctcgttta
    10561 ttagaacgtt tgcaataagg ggttcccatg tttacatcag tggcgcaagc caatgctgcg
    10621 gttatcgaac aaattcgtcg cgctcgtcca cactggctgg atgtgcaacc ggcttcttca
    10681 cttatcagcg aactaaacga gggcaaaaca ctgcttcacg ccgggccgcc aatgcgctgg
    10741 caggagatga ccggacccat gaaaggggcg tgcgtgggcg catgtctgtt cgaaggctgg
    10801 gcgaaagatg aagcgcaggc gctggcaata ctggagcagg gggaagtgaa cttcattcct
    10861 tgtcaccatg tgaatgccgt cgggccaatg ggcggcatta cttctgccag tatgccgatg
    10921 ctggtggtcg agaacgtgac cgacggcaac cgggcgtact gcaacctcaa cgaaggtatc
    10981 ggcaaagtga tgcgttttgg cgcttacggc gaagatgtcc tgactcgcca tcgctggatg
    11041 cgcgatgtgt taatgccagt attaagcgcg gcgctggggc gcatggagcg cggtatcgat
    11101 ctcacggcga tgatggcgca gggcattacg atgggcgatg agttccatca acgcaatatt
    11161 gcttcctctg cactgttaat gcgtgcgctg gccccacaaa ttgctcgcct cgatcatgat
    11221 aaacagcaca tcgccgaagt gatggatttc ctcagcgtga ccgatcagtt cttcctcaac
    11281 ctcgcgatgg cttactgcaa ggcggcgatg gatgctggcg cgatgatccg tgcaggcagc
    11341 atcgtcacgg caatgacccg caacggcaat atgttcggga ttcgggtaag cgggctgggc
    11401 gaacgctggt ttactgcgcc tgtaaacact ccgcaaggtc tgtttttcac cggcttctcg
    11461 caggagcagg cgaacccgga tatgggcgat agcgcgatta ccgaaacctt tggtatcgga
    11521 ggtgcggcaa tgatcgcagc gcctggcgta acgcgctttg tcggtgcggg tggcatggaa
    11581 gcggccagag cggtatctga agagatggcg gaaatttacc ttgagcgcaa tatgcagttg
    11641 cagatcccag gctgggattt tcagggcgcg tgcctgggac tggacattcg tcgcgtggta
    11701 gaaaccggca ttacgccact catcaatacc ggtatcgccc ataaagaggc ggggatcggg
    11761 cagattggcg caggcactgt gcgggcaccg ctggcgtgtt ttgaacaggc gctggaagca
    11821 ctggctgaaa gcatggggat tggttgagga acgcgcaatg acgatcatcc atcctctgct
    11881 tgccagccgt agtgcaccga attatcgcca gtcctggcgg ttagcgggag tgtggcgacg
    11941 ggcgattaac ctgatgacgg aaagcggcga actgttaacg ctgcatcgtc agggtagtgg
    12001 tttcggcccc ggaggatggg tgcttcgccg cgcgcaattc gatgcgttat gcggtggatt
    12061 atgcggcaat gaacgaccac aggttgtggc tcaagggatt cgcctcgggc gtttcacggt
    12121 taaacagcca cagcgttatt gtttgctgcg tattacgccg ccttcacatc ctcaaccgct
    12181 tgcagctgca tggatgcaac gcgcggagga aaccggactt ttcggaccac tggcgatggc
    12241 ggcaagcgat ccgctgaccg ctgaattacg ccagtttcgt cactgttttc aggccgcgct
    12301 caatggcgtt aagaccgact ggcggcactg gctgggtaaa ggccccggat taacgccaag
    12361 tcatgatgac acgttgagcg gaatgctgct ggcggcctgg tattatggcg ctttagatgc
    12421 gcgctccggt cgccagtttt ttgcctgctc cgacaatctt caactcgtta ccacagcggt
    12481 gagcgtcagt tatttacgct acgccgcgca aggatatttc gcctcgccac tcctgcactt
    12541 tgttcatgct ctgagttgcc cgaaacgtac cgctgttgcg attgattcgc tgctggcgct
    12601 ggggcacacg tcaggggcag atacgctgct ggggttctgg cttggtcaac aattattaca
    12661 aggaacacca tgaaaacact ggttgtagct cttgggggca acgccttact ccagcgcggt
    12721 gaggcgctga cggcagaaaa tcaatatcgc aatatcgcca gtgctgtacc cgcgctggcg
    12781 cgcctggccc gttcttatcg gctggcgatt gttcacggca atgggccgca ggtggggctg
    12841 ctggcattgc agaatctggc gtggaaagag gtagatccgt atccgctgga tgtgctggtg
    12901 gcggaaagcc aggggatgat tggctatatg ctggcgcaga gtttgagcgc acagccgcag
    12961 atgccgcctg tcacgacggt gctgacgcgc attgaggttt cgcctgatga tccggcgttt
    13021 ttgcagccag agaaatttat tggtccggtt tatcagccag aagaacaaaa ggcactggaa
    13081 gcggcttacg gctggcagat gaaacgtgat ggtaaatatt tgcgccgggt ggtggcgtct
    13141 ccgcaaccgc gtaaaattct cgacagcgaa gccatcgagt tgttgctcaa agaggggcat
    13201 gtggtgattt gcagtggcgg cggcggtgtg cctgtgacgg aagatggggc agggagtgaa
    13261 gcggtgattg ataaagatct cgccgctgcg ttgctcgccg agcagattaa tgccgatgga
    13321 ctggtgatcc tcaccgatgc tgacgcggtt tatgaaaact ggggaacgcc gcagcaacgc
    13381 gccattcgcc atgccacacc ggatgagtta gcgccatttg ccaaagccta tggttcgatg
    13441 gggccgaagg taacggcggt aagtggttat gtcagaagcc gtggtaaacc cgcgtggatt
    13501 ggggcgttat cgcgaattga agagacgctg gcgggcgaag cggggacctg tatttcgctg
    13561 tagtgatgag agcatccggc aatcggtgca ccatgcctga tgcgacgctt gacgcgtctt
    13621 atcaggccta caatgggtac cggagcggta agccggataa ggcgtttacg ccgcatccgg
    13681 caatcaaatg agcacctgct taactgaact tactctgcgc ccacatcacg ccgctggcat
    13741 actccggcgg cagcagcggg atcaaggctt ccagcgtcgc ggtcagacgc gatgtgtcgc
    13801 tgtcggtcaa attcagatgc cccactttac gccccggacg gacttctttg tcgtaccagt
    13861 gcagatgcac cagcggcagt ttcagccagt cataattcac atcgctacca atcaggttga
    13921 tcatcaccga cggactattc actaccggtt gcggtaacgg cagatcggta atcgcccgca
    13981 gatgcagctc aaactggctg atgctggcac cgttttgtgt ccagtgaccg ctgttatgca
    14041 cacgcggtgc cagttcgttg atcagcagac cttgcggggt gacaaaacac tccatcgcca
    14101 tcacgcccac atagcccagc tcctgcataa tcgccgacag catctcttcg gcttgcgctt
    14161 gctgctgcgc attggcctgc ggaaaagcga cgctggtgcg caaaataccg tcctgatgca
    14221 ggttatgcgt cagcggataa aacacggtgc tgccatcaaa tccgcgtgcg ccaaccagcg
    14281 acacttcacc agagaagtta atgccctgct cgacaataca ttcgccgtaa cactctgccg
    14341 gtaactgttc ggtttcattg gcacgtaaac gccattgacc gcgaccgtca tagccaccag
    14401 tgcgacgctt aacaatcgcc agttcaccta aacgctcaaa caccgcaggc cactcgctgc
    14461 gatcggcaag taactgccac ggtgcggtcg gcaggtggag cttatcgaaa agctgcttct
    14521 gagtcagacg gtcggcaata atcgggaaca catcgcggtt cacaaaggcc ggatgacgcg
    14581 ccagctcgcg ggttaatgcg gtttccggcc agcgttcgat ctcagcggta atcacgcttt
    14641 gttgaaaagg caccgccgcc ggttcagcgt ccagcccgac cggccagaca gcaatgccta
    14701 acggttcacc tgcctggcgc agcatacggc ctaactgccc gttaccgagg acgcaaacct
    14761 gtttcatgcc gcacctcgcg ggtccgggtt ttccagcact tcgtcggtct gggctttgcg
    14821 ccagtcattc agacgctggt gcagttcttt atcatgagtc gcaagaattt gtgctgccag
    14881 taacgccgcg tttgccgcgc cagctttacc aatcgccagc gtacccaccg gaatgccgcg
    14941 cggcatttgt acgatggagt agaggctatc gacaccgctc agtgcggcgc tctgtactgg
    15001 cacgcccagc actggcacca gcgttttggc ggcaatcata ccaggcaggt gcgctgcgcc
    15061 gcctgcgccc gcaataatca cctgataacc gttctcttcg gcgctttcgg cgaagctgaa
    15121 cagcttatcg ggggtgcggt gagcagaaac cacttcaacg tggtgcggga cattcaggat
    15181 ttcgaagatt tcggcggcga actgcatggt agcccagtcg cttttggacc ccatcacgat
    15241 ggcgacacgc gccggattat tgcgggaaga catgcgtctt aaaactcctg tggtgcacaa
    15301 ctctcggctt tagagggcac agagaatagc acggaaagag agcaaggaaa acggttgcgt
    15361 ggctgtgaaa tcagcaaagt tgcgggtttt ttaaaacgga aaatgaatca gctcaacgtc
    15421 atccgccgtg actttcacca tcgaaccttc cgtatgccag gcaccgagta ccacgcgaaa
    15481 agcaggttgc tggttggcga taagttcatg taccgccggg cgatgggtat gtccgtggat
    15541 cagccattgc acctgatgtt tttccatcgc actgactacc gcgttttggt taacgtccat
    15601 gatcgccagc gatttactgc tgttggcttc tttactgttc gctcgcattc gcgcggcaat
    15661 gcgtttgcgc acaaacaacg gcagggcgag gaatagcatt tgcagccagg gtttgtggac
    15721 tttggcgcga aaagcctgat aacccgcgtc atcggtgcac agcgtgtcgc catgcataat
    15781 caacacccgg cgaccataaa gttcgagcac cttttcttcc ggcaataatg tcatgccgct
    15841 ttcacgggca aagcgtttgc cgagcagaaa atcacggttg ccatgaatga aataacaggg
    15901 aacgccggaa tcggacaccg ctttgatcgc tgccgccatc tggcgatgga gtgggttggg
    15961 atcgtcgtcg ccaatccatg cttcaaacag atcgccaaga atatacagcg cttcggcctt
    16021 gcgggcttcc cccgctaaaa aacgcagaaa accggcggtg atcgccggtt cttccacgca
    16081 gagatgaaga tctgcaataa agagtgtcgc cacgattact cgctaacggt cacgctttca
    16141 atgataacgt cttcttttgg cacgtcctgg tgcataccgc tacgaccggt tgcaacacct
    16201 ttgattttgt ctaccacgtc catgccttca accacttcag caaacacgca gtagccccaa
    16261 ccttgcaggc tttcgccaga gaagttcagg aagtcgttat caaccacgtt gatgaagaac
    16321 tgtgcagttg cagagtgcgg agcctgagta cgggccattg ccagcgtacc acgggtattt
    16381 ttcagaccgt tgttggcttc gtttttgatc ggttctttgg tggctttttg tttcatgccc
    16441 ggttcaaaac cgccgccctg aatcataaag ccgttgataa cacggtggaa aatggtgttg
    16501 ttgtaaaaac cttcgcggca gtagtccagg aagtttttaa ctgtttcagg tgctttatcg
    16561 tcaaaagttt tgatgacaat atcgccgtga ttggtgtgga aagtaaccat ttttgcatcc
    16621 tgttccgttt gattggtgct tcaacccagt tcgggtcata tatagggtgg tgttatagca
    16681 taaccgcacg atcggatcat cacgcaatgt atgctgattc gcgcgggaaa tatgggtatt
    16741 atacgcaact caattaccca cacatgtcta aacggaatct tcgatgctaa aaatcttcaa
    16801 tactctgaca cgccaaaaag aggaatttaa gcctattcac gccggggaag tcggcatgta
    16861 tgtgtgtgga atcaccgttt acgatctctg tcatatcggt cacgggcgta cctttgttgc
    16921 ttttgacgtg gttgcgcgct atctgcgttt cctcggctat aaactgaagt atgtgcgcaa
    16981 cattaccgat atcgacgaca aaatcatcaa acgcgccaat gaaaatggcg aaagctttgt
    17041 ggcgctggtg gatcgcatga tcgccgaaat gcacaaagat tttgatgctt tgaacattct
    17101 gcgcccggat atggagccgc gcgcgacgca ccatatcgca gaaattattg aactcactga
    17161 acaactgatc gccaaaggtc acgcttatgt ggcggacaac ggcgacgtga tgttcgacgt
    17221 cccgaccgat ccgacatatg gcgtgctgtc gcgtcaggat ctcgaccagc tgcaggcagg
    17281 tgcgcgcgtt gacgtggtcg acgacaaacg caacccgatg gacttcgttc tgtggaagat
    17341 gtcgaaagag ggcgaaccga gctggccgtc tccgtggggc gcgggccgtc caggctggca
    17401 cattgaatgt tcggcaatga actgcaagca gctgggtaac cactttgata tccacggcgg
    17461 cggttcagac ctgatgttcc cgcaccacga gaacgaaatc gcgcagtcca cctgtgccca
    17521 tgatggccag tatgtgaact actggatgca ctcggggatg gtgatggttg accgcgagaa
    17581 gatgtccaaa tcgctgggta acttctttac cgtgcgcgat gtgctgaaat actacgacgc
    17641 ggaaaccgtg cgttacttcc tgatgtcggg ccactatcgc agccagttga actacagcga
    17701 agagaacctg aagcaggcgc gtgcggcgct ggagcgtctc tacactgcgc tgcgcggcac
    17761 agataaaacc gttgcgcctg ccggtggcga agcgtttgaa gcgcgcttta ttgaagcgat
    17821 ggacgacgat ttcaacaccc cggaagccta ttccgtactg tttgatatgg cgcgtgaagt
    17881 aaaccgtctg aaagcagaag atatggcagc ggcgaatgca atggcatctc acctgcgtaa
    17941 actttccgct gtattgggcc tgctggagca agaaccggaa gcgttcctgc aaagcggcgc
    18001 gcaggcagac gacagcgaag tggctgagat tgaagcgtta attcaacagc gtctggatgc
    18061 ccgtaaagcg aaagactggg cggcggcaga tgcggcgcgt gaccgtctta acgagatggg
    18121 gatcgtgctg gaagatggcc cgcaagggac cacctggcgt cgtaagtaat tgcgctgttg
    18181 ccggatgcga gttttcgcat ccggttatcg cctgcgccac cacaacattc ccataagcac
    18241 catccccggc aaccacaccc acatcaattc agaaattatc acctgatgcc cgtacggcgt
    18301 ggtgtaacga gacaatgcaa acggcgcgac tttaatcacc tgccagggag cgaaaaagcg
    18361 ttcatctgac cacggccaca gccagccaac gcccttaccg ccagtggtta ccgaatccag
    18421 caagctgtgc gatagcaacg agacggttaa aaacagccag cagcgaatca gcccagccct
    18481 gaaccatcgg cgtccaatca gcacacataa cagcgggaca acaaacgcaa acaccagcga
    18541 atgggtaaac ccgcgatgac caaaaacatt gccgtaagca acgccaaatt taaacgacaa
    18601 tacgtcggcg tcgggcagca tcgccaggat gattccggca aataacagac gcggagggat
    18661 gactttcgaa cccaacccta aaccaatgca taggggaacg gcggcgtgcg taataacggt
    18721 tggcatgatg gtcgcttcgg caaaatgtcg atgctatcag catggatgaa cggggcgtag
    18781 agggcaaaag tctgaaaaga gaaccggcct gttgatacag gccgggaaag ggatcaggca
    18841 acaacctgta cgctgtgacc tgcaaaactc actgtctgac cggcgacgat tttgcagcgt
    18901 ttgcgcgttt caaccgcacc gtcgactttc acctggcctt cggcaatcgc gattttcgcc
    18961 tgcgcgccgc tttcgctcca gccttccagt ttcagcaagt cgcacagctc aacgtgcgga
    19021 tgtttaccta aagaaaatgt cgccatgtta ctcatcctgt ggatcatgat attcaacgca
    19081 cgcctgtagc gtgttttcaa tcagcgtggc aaccgtcatc gggccaacgc cgccgggaac
    19141 aggcgtaatg tatgaggcgc gtttagccgc gtcttcaaac acgacgtcgc ccacaacttt
    19201 gccattttcc agacggttga tgccgacatc aatcacaatt gcgccttctt tgatccagtc
    19261 accgggaata aagcctggct tgccaacggc aacgatcaac agatcggcat tttctacgtg
    19321 atgacgcaga tttttagtga agcggtgagt cacggtagtg gtgcaacctg cgagcaacag
    19381 ttccatgctc attgggcgac cgacgatatt cgatgcgcca atcaccacgg cgttgaggcc
    19441 gaaggtatca atgttgtaac gctcaagcag cgtgacgata ccgcgcgggg tgcagggacg
    19501 cagacgcggc gcgcgctggc acagacgacc gacgttgtaa ggatggaaac cgtccacgtc
    19561 tttgtccgga tgaatacgtt ccagcacttt gacgttatca atacccgccg gtaacggcag
    19621 ttgaaccaga atgccatcga tggtgttgtc ggcattcagc gtatcgataa gctccagcag
    19681 ctccgcttcg ctggtggttt ccgggaggtc ataagagcgg gagacgaacc cgacttcttc
    19741 acaagccttg cgtttgcttg cgacataaat ttgcgatgca gggttactac ccaccagcac
    19801 aacggccagt cctggtgccc gcagtccggc tgcaatacgc gcctgaactt tttgagcaac
    19861 ttcagagcgc acctgctgcg caatcgtttt accgtcaata atctttgctg ccatcagaga
    19921 gaggattcca tctgttacgt aggtcgaagg ggatgcgtct attttgtcag aagcggggcg
    19981 cgctgtcagg tttcgtttca gatttatcgc gtgaagcgac ctcttgcgaa ggttaggcgc
    20041 accgtcgctg aggtcgctga gattgaaagc ttcatttttc gtccatgatg gcgttgtaaa
    20101 tctggaactg
//
LOCUS       DAZFWP020000090        20072 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFWP020000090 DAZFWP020000000
VERSION     DAZFWP020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.090"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(131..877)
                     /gene="cutC"
                     /locus_tag="ACHK0Z_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             complement(131..877)
                     /gene="cutC"
                     /locus_tag="ACHK0Z_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU4503076.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(891..1457)
                     /gene="yecM"
                     /locus_tag="ACHK0Z_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             complement(891..1457)
                     /gene="yecM"
                     /locus_tag="ACHK0Z_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4503077.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1673..3406
                     /gene="argS"
                     /locus_tag="ACHK0Z_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             1673..3406
                     /gene="argS"
                     /locus_tag="ACHK0Z_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU4503078.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3448..3492)
                     /gene="yecV"
                     /locus_tag="ACHK0Z_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             complement(3448..3492)
                     /gene="yecV"
                     /locus_tag="ACHK0Z_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HGU4503079.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3583..4071
                     /gene="yecT"
                     /locus_tag="ACHK0Z_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             3583..4071
                     /gene="yecT"
                     /locus_tag="ACHK0Z_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4503080.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4191..4583)
                     /gene="flhe"
                     /locus_tag="ACHK0Z_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             complement(4191..4583)
                     /gene="flhe"
                     /locus_tag="ACHK0Z_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU4503081.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4583..6661)
                     /gene="flhA"
                     /locus_tag="ACHK0Z_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             complement(4583..6661)
                     /gene="flhA"
                     /locus_tag="ACHK0Z_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4503082.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLESLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6654..7802)
                     /gene="flhB"
                     /locus_tag="ACHK0Z_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             complement(6654..7802)
                     /gene="flhB"
                     /locus_tag="ACHK0Z_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU4503083.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8004..8648)
                     /gene="cheZ"
                     /locus_tag="ACHK0Z_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             complement(8004..8648)
                     /gene="cheZ"
                     /locus_tag="ACHK0Z_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU4503084.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8659..9048)
                     /gene="cheY"
                     /locus_tag="ACHK0Z_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             complement(8659..9048)
                     /gene="cheY"
                     /locus_tag="ACHK0Z_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU4503085.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9063..10112)
                     /gene="cheB"
                     /locus_tag="ACHK0Z_RS15515"
                     /old_locus_tag="pgaptmp_003103"
     CDS             complement(9063..10112)
                     /gene="cheB"
                     /locus_tag="ACHK0Z_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU4503086.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10115..10975)
                     /gene="cheR"
                     /locus_tag="ACHK0Z_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             complement(10115..10975)
                     /gene="cheR"
                     /locus_tag="ACHK0Z_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU4503087.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLEKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10994..12595)
                     /gene="tap"
                     /locus_tag="ACHK0Z_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             complement(10994..12595)
                     /gene="tap"
                     /locus_tag="ACHK0Z_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU4503088.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12641..14302)
                     /gene="tar"
                     /locus_tag="ACHK0Z_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             complement(12641..14302)
                     /gene="tar"
                     /locus_tag="ACHK0Z_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU4503089.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSTFRLAASPLTNKPQTPSRPASEQPPTQPRLRIAEQDPNWETF"
     gene            complement(14447..14950)
                     /gene="cheW"
                     /locus_tag="ACHK0Z_RS15535"
                     /old_locus_tag="pgaptmp_003107"
     CDS             complement(14447..14950)
                     /gene="cheW"
                     /locus_tag="ACHK0Z_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU4503090.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14971..16935)
                     /gene="cheA"
                     /locus_tag="ACHK0Z_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             complement(14971..16935)
                     /gene="cheA"
                     /locus_tag="ACHK0Z_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU4503091.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16940..17866)
                     /gene="motB"
                     /locus_tag="ACHK0Z_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             complement(16940..17866)
                     /gene="motB"
                     /locus_tag="ACHK0Z_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU4503092.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17863..18750)
                     /gene="motA"
                     /locus_tag="ACHK0Z_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             complement(17863..18750)
                     /gene="motA"
                     /locus_tag="ACHK0Z_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4503093.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18877..19455)
                     /gene="flhC"
                     /locus_tag="ACHK0Z_RS15555"
                     /old_locus_tag="pgaptmp_003111"
     CDS             complement(18877..19455)
                     /gene="flhC"
                     /locus_tag="ACHK0Z_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU4503094.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19458..19808)
                     /gene="flhD"
                     /locus_tag="ACHK0Z_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             complement(19458..19808)
                     /gene="flhD"
                     /locus_tag="ACHK0Z_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU4503095.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4789 a         5531 c         4906 g         4846 t
ORIGIN      
        1 acccgctcaa cagatcacgg ttatcgttcg ttttttatac tgctcaggga taaaaaaagg
       61 cccctgttga aattgcaggg gcctggtacg agcaagcatc atattgggcg acatgatgca
      121 acggtaaaaa tcatttggcc tgatggcgtt caatgattcc tttcatttca gcaaccgccg
      181 ccccgtctac gatataacgc gaatactcgt ccgcgtgttc atctgatgac atggacaatc
      241 cttgattacg ataacgcatc ggtgaggctt gccacgctcc cgcggagcta tggacttcca
      301 gcactccggc atcgaggaag tggtgcaggt tttctgcacg gactcctgct ccggccataa
      361 tgattggagc atcacgatgg gcaataagtt ccataatttt tgataaacct tgcagtgcgt
      421 ctgatttttg ccctgatgtc agtacccggg caatgcccaa ttccgcaaga ttattgagtg
      481 tatataaagg gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg
      541 cagcagccat tattttttcc attcgtggca tatcgacatt cccgtcaaca tcgagaacgc
      601 ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca agaatggcgg
      661 caaactcacc gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc ggatgcacag
      721 ggatcgtcac ccgctggcgc acggatttca gtacccccaa cgacggcgtt aagcccccct
      781 cttttggggc tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac
      841 attccatgct gtaacagcaa atttccagta atgccatttt tgctccttaa ttacgctgac
      901 tgctcgctgg caacgatctc ttcaatggac cacggatgaa acttaatagt tgttttgcca
      961 tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct caccttttgg tgaactggtt
     1021 ttaactgaga tccccggtag gctgagtcct tcatcagaaa gcaatgccag cgcacgggca
     1081 ttcagcgttt ctggatcacc cggcagaacg atttcaatat gttcccaacc ttcgtgtggg
     1141 taacgttttt ccccgggcca cggtagctcc acaatagaaa actgccagtg cgcaacctgt
     1201 accggttcat gcaatttaaa cagacaaatc ggtctgccat tgatcatatt ttctgacaaa
     1261 agctcgccac actgttcaaa cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa
     1321 cgcaaagaaa tgtgatcggc agtcagcgga gtgatattca accccagacg gagggaaagt
     1381 tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata
     1441 gattgccagt tcgccataat cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt
     1501 gccctgcgcc aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa
     1561 tacgccacgc gcacgttgct gacgaaaaca gccatttgca gtatactccc gccctaattt
     1621 ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc cggtgaatat
     1681 tcaggctctt ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga
     1741 ttgcgaaccg caggttcgtc agtcagcaaa agttcagttc ggcgactatc aggctaacgg
     1801 catgatggca gttgctaaaa aactgggtat ggcaccgcga caattagcag agcaggtgct
     1861 gactcatctg gatcttaacg gtatcgccag caaagttgag atcgccggtc caggctttat
     1921 caacattttc cttgatccgg cattcctggc tgaacatgtt cagcaggcgc tggcgtccga
     1981 tcgtctcggt gtttctacgc cagaaaaaca gaccattgtg gttgactact ctgcgccaaa
     2041 cgtggcgaaa gagatgcatg tcggtcacct gcgctctacc attattggtg acgcagcagt
     2101 gcgtactctg gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg
     2161 cactcagttc ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa acgccggtga
     2221 aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt acgacgaaga
     2281 tgaagagttc gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg gtgacgaata
     2341 tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc agatcactta
     2401 cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc tctacaaccc
     2461 aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag aaagcgaagg
     2521 ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa ggcgaaccga tgggcgtgat
     2581 cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct gtgcgaaata
     2641 tcgttatgaa tcactgcatg ccgatcgcgt gctgtattac atcgactccc gtcagcatca
     2701 acacctaatg caggcatggg cgatcgtccg taaagcaggc tatgtaccgg aatccgtacc
     2761 gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt tcaaaacccg
     2821 cgcgggtggt acagtgaaac tggccgatct gctggatgaa gccctggaac gtgcacgccg
     2881 tctggtggca gaaaagaacc cggatatgcc agctgacgag ctggaaaaac tggctaacgc
     2941 ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca cggactacat
     3001 cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca tgcagtatgc
     3061 ttacacgcgt gtattgtccg tgttccgtaa ggcagaaatt aacgaagagc aactggctgc
     3121 agctccggta atcatccgtg aagatcgtga agcgcaactg gcagctcgcc tgctgcagtt
     3181 tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtgatgt gtgcttacct
     3241 gtacgatctg gctggtctgt tctctggctt ctacgagcac tgcccgatcc tcagcgcaga
     3301 aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga agacgctaaa
     3361 gctgggtctg gatacgctgg gtattgagac agtagagcgt atgtaatcga tttttcgtga
     3421 gagtaaagcc tgatcagggt tacctgatca ggctttttta ttgccatcta aatgtattct
     3481 gaaaatggac atgccattgt tttctgactg ttggataaga ggccagaagc gtaatatccg
     3541 gccccaggga aacgataacg gttgaattta aggaataccg cagtgtttaa atttcttgta
     3601 ttaacattag gcattatctc ttgccaggct tacgcagaag atacggttat agtaaacgac
     3661 catgacattt cagccatcaa agattgttgg caaaaaaatt cagatgatga tactgacatt
     3721 aacgtgatca aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca attaaataaa
     3781 gcctatggtg aagcttatcg ttatatagaa caagtgccac gcacaggtgt aaaaaaacct
     3841 gataccgaac aacttaactt gcttaaaaaa tcacagcgag cctggctgga ttttagggac
     3901 aaagaatgtg aattaatcct ttcaaatgag gacgttcagg atttaagtga cccttattct
     3961 gaatcagaat ggctctcatg tatgatcata cagaccaata cgcgaactcg ccagttgcag
     4021 ctataccgta actctgaaga tttttatcca agccctttga caagaggata attcacatct
     4081 ttttggcatg ttttgttgca agctattcct gataaataat tgcaacaaga catcgagcct
     4141 ttttcactga gttattaaac atactcgcga gcgcgtaatt tttttgtcct tcagcgataa
     4201 ttcacaatca cttcattacg ttgtaccttt agcggtggaa ttaaccgccc accgcctggc
     4261 acttcccaga taaatcgcaa cggttctgct gccgctatac cggaaaaggc cacggtggtt
     4321 ccgctctgcc cctctaattc gacacaacga gattgcgaac acaagcgaac ccgcagtcct
     4381 gaaggtgtcg ggccgataag ctgataacgc cacgctacca gcgtcatcaa tcctgaagca
     4441 ggttgtcgcg tagaaagagg cgcagacgac atcgactcac cgcgatgatt tagcgtaatt
     4501 cctacactac ttgcctgcca catcccctcc ccggcggctt gcaccagcag cggaaacaat
     4561 aatattgcta ataaggttct cattatttgc cgccaattgt cgccgtcatg cggatatgtc
     4621 ggttatcaga cagttccaga ttcgacagga ccactaactg cggcaagctg cggcgcagga
     4681 agcgagacaa taatggtcgc agcgcgtggt tcaccaacaa tactggcggc gcacccagca
     4741 tctcctgacg ggatagcgct tcctgagttt gcgccagtaa acgatccgcc agccctggct
     4801 ccagtcctcc cccgccctgc agcgcctgta gtaacaaact ttccagcggt gtatcgaggc
     4861 caataacatg gacttcatct ttgccaggaa accactgctg ggtaatcgcc cgtcccaacg
     4921 ccacgcgcac gacggcggtt aattcatgtg gatcgctttg gatgggcgca tgttccgcca
     4981 gcgtttcgag aatggtgcgc atatcgcgaa tcggtacttt ttcatcgagg agattttgca
     5041 gcactttatg cagtgtggtg agcgtgacga cgccaggaac gagatcttcc gtcagctttg
     5101 gcatctcctg ggcgacgcga tccaacagct gttgcgcctc ctgacgacca aacagctctg
     5161 cggcatgctg gctaatgagg tggttaagat gcgttgctac caccgtgctg gcctcaacca
     5221 ctgtgtaccc ctgaatctgc gcctgttctt ttagcgcact ttcaatccag atagcattca
     5281 ggccaaatgc cggatcgacg gtcgcctcac caggtaacgt cccggcagcg gttccagggt
     5341 taatcgccag ccagcgcccc ggataagcat caccactgcc aatctccacg cctttcatca
     5401 aaatgcgata gcgggcaggt tgcagatcca tattgtcgcg aatgtgcacc actggcggca
     5461 gaaatcccat ctcctgggca aatttcttgc ggatactgcg tatacggccc aacaactcac
     5521 catcctgctg gaaatcgacc atcgggatca gtcgataacc cacttccatt cccagagaat
     5581 cttccagttg tacatcgttc cacgtcgctt cgacaacggt attattctct gccattttta
     5641 ccggtttggg ttcgacaggc gctttttgtt cgcgtccgcg tatccaccag gccagcccca
     5701 gcaatccggc agtgaacagc aaaaatacca ggttcggcat tccaggcacc aggccgagta
     5761 aaccgagcac ggcggcgctt aacaacataa cgcttgggtt actgaaaagc tgattcacca
     5821 tctgctcgcc aacatcctga tcggtgctga cacgcgtaac gatgaccccc gcggcggtag
     5881 aaatcaccag cgccggaatt tgtgccacca gaccgtcgcc aatggtcaat agtgtataac
     5941 tttccgccgc gtgtcccatg ctcatgccat gttgcagcac gccgaccagc aacccgccga
     6001 caacgttaat gaccatgatg aggatcccgg cgatggcatc gccgcgaaca aacttacttg
     6061 ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg cgttttttcg
     6121 cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc tgcttacccg
     6181 gcataccatc gagaacaaag cgcgcaccca cttctgcgat acgcccggca cctttggtaa
     6241 tgaccataaa gttgatgatc acgagaatga caaacactac gataccgata gcgaaattgc
     6301 caccaacgag gaagtgaccg aatgcttcga ccaccttccc agccgccgcc gcgccggtat
     6361 gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc aacaacgtag
     6421 taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta aacatcgcca
     6481 ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg agtatgaatg
     6541 cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt ccggcaagga
     6601 tctgccattg tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc agattactca
     6661 tgggtcggtt tctcgttaat aaaatccagg gcttccggca ccggaagatg agtaggttgt
     6721 acagggcgct gtccaccagc cagacgccag cgtttcagtt gccagaccca ggccagcact
     6781 tccgccaccg cggcgtacag ttgacccggg atttgttgac caatctccgc atgtcgatac
     6841 agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc gccaatttca
     6901 cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc gctcattttg
     6961 ttttcgtcat actgcaacgc taccgaatag tgggtcgggt tattgacaat gacatccgct
     7021 ttcggcacat cggccatcat ccgacgccgt gcagcagctc gctgcatctg acggatccgc
     7081 cctttaacat gagggtcacc ttcgctttgt ttgaactcat cacgaatatc ctgccgtgac
     7141 atacgcagct ttttcaggtg gctgaatatt tggaaaaaga cgtcaaatcc caccattgga
     7201 atgacaccaa gcaccaccag cagtgcgcat agccctacca aatccatcgc attacccatg
     7261 gcggtaatcg gagactcggc catcaagcgc atcatctgcg gccagtgatg ccagagaaaa
     7321 aaccccgtca cgctgccaac caggatggtt ttcaaaattg ctttaagcaa ctccgcgcca
     7381 gtctgagccg agaacatccg tttaatgccc ggtagcgggt tgagtttgga aaacttcggc
     7441 tgcaaggatt tgccgctaaa taccagccct cccagcatga ccggagaaat gagcgccacc
     7501 agcaccacgc cgctaatcag cggcagcagc gccagcatgg cttctctgat cagcagaata
     7561 atctgcccga ggatcagatt cgggtcattg ataatactgt gatcaaaatg cagcccagcg
     7621 gagagcatgc ccgacaatcg acgggccagc gacacaccgc caaaccagat aacactaacg
     7681 cccaccagca aaatcagcag tgaggtcagt tcacgggaac gcgggatttg cccctcttcc
     7741 cgcgcttttt ctagtcggtg aggtgtgggg gcttctgttt tgtcgtcgct ctcgtcagac
     7801 acgtcgccaa tcctggaaag agtattaagc gtgaatgatg ccagagcgca aagcgttcaa
     7861 tggtttgagt aaggggcaaa acaggcggga tttagggctt ttgctgccac acatcaagca
     7921 tagtgtgcgt ttgtcggatg cggcgtcatc gccttatcag accgcctgat atgacgtggt
     7981 cacgccacat caggcaatac aaatcaaaat ccaagactat ccaacaaatc gtccacctga
     8041 tcctgactgg ctaccacgcc ggctttgctg gtatcgacct gaggtccatt aagcaaactc
     8101 tggttttcac gttttggacg cgactcctgt tccgggatgt tttccaacag caccatcagc
     8161 aactggcgtt cgatctcctg aatgacatcc atcatccgct taatgacctg cccggtgaga
     8221 tcctgaaaat cctgcgccat catgatttcc agcagttgcg cgttagtaaa gctggtatgc
     8281 gcgggtacat ctgccagaaa ttgtcgtgta tctgttacca gttcacgggc gtcggcaagg
     8341 tcaatcggat cggcaaacca gtcatcccaa cgttgggtta acgcttttgc tgatttctcc
     8401 atttgatcct gatgcggttg tgacgcctca acactgttca gcgcccgctc cgcagcctgg
     8461 gcggtcatct gcacaacata gtacaaacga tcgcgcgcat cggggatggc ttccgccgct
     8521 tcggcaatgg cctgatccag ccccagttcc cgcaaactgt cgcgcagcat acgcgtcagg
     8581 ctgccgatgc gcgcaatgat atcgccagct gaatgctcgt cagcaggttt gattgatggt
     8641 tgcatcatag tcgcatcctc acatgcccag tttctcaaag attttgttga gtttttcctc
     8701 cagcgtcgcg gcggtaaatg gcttcaccac atagccactg gcccccgctt gcgccgcagc
     8761 aatgatgttc tctttcttcg cttctgcagt caccattaac actggcaatg ccgacatcgc
     8821 gccatccgca cgaattgttt tcagcaattc caggccatcc atattgggca tgttccagtc
     8881 ggagataaca aatccataac cgcctgcctg caacttattg agagcgtcga cgccatcttc
     8941 cgcttcctca acattattga atcccagctc tttaagcagg ttacgcacta tgcgtcgcat
     9001 ggtggaaaag tcatccacaa ccaaaaattt aagttcttta tccgccattt cacactcctg
     9061 atttaaatac gtatcgcctg tccggcacta atttttgcca acatttgctg gcttacctgg
     9121 ctaagatcga ccacttcgca gacaccaccc atattgatgg cctcgcgcgg catgccgaac
     9181 accacgcaac ttgcttcgtt ttgcgcaagg gtccatgccc ccgcctgacg catcgccaac
     9241 attcccgccg cgccgtcgtt gcccataccg gtcaggatca ccccaaccgc attacgcccc
     9301 gcctgtttgg cgacagaatg gaacaacaca tctaccgaag gccgatgacg gttaaccgcc
     9361 gggccatcgt gaattttgat ttggtaattt gcgccactac gcgacagctc catatgccga
     9421 tcgcccggcg caatataggc atgccccggc aagacacgtt ctccgtcttc ggcttcttta
     9481 accccgatct ggcaaagctt attaagtctg tcggcaaaag agcgggtgaa accgggcggc
     9541 atatgttggg taattaacaa tgccgggctg gaaagcggca acggttgcag tacgtgacga
     9601 attgcctcag ttccacccgt tgaagcacca atcgcaatca gtttttcaga actcaacaac
     9661 ggacccgcct tcagcgttgt cggtgctgac aatggcttat gtgctgcaag gctcgccttt
     9721 gctgccgtac gcaccttttc agcaatcatt tcgctatacg ccagcattcc ttcgcgaata
     9781 cccagttgcg gtttggtgac aaaatctatt gcccccagct ccagcgcgcg cagcgtgact
     9841 tctgatcctt tgccggtcaa ggaagaaacc atcacaacgg gcattggacg caaacgcatt
     9901 aatttttcga ggaaatccag tccgtccatc cgcggcattt caacatccag cgtcagcaca
     9961 tcgggattga atttcttaat caagtcacgc gcgaccagcg gatcaggcgc ggtcgccacc
    10021 atttccatgt cgctatggct gttgatgatt tctgtcatga tctggcgcat cagtgccgaa
    10081 tcatcgacag ataacaccct gattttgctc atcgttaatc cttacttagc gcatacaccg
    10141 tctgaccacg cagcgtgaag cggcgctcaa ggtggctaaa gttttcagag tgacccgcaa
    10201 acagcaatcc gtcgggttta aggagcggaa caaagcggcg caaaatctcc tgctgggtat
    10261 tttgatcgaa gtagatcatg acgttacgac agaagatcgc atcaaacggc cccggcacgg
    10321 tgtactgttt ctccagtaga ttcagcgggg caaaatcaac atagttcgcc aactcctgac
    10381 gcacgcgtac cagcccttca tgcggtcccg tccctcgcat gaaataccgt tgcagttgct
    10441 gcggcgtcag gtttttcaac tcttcatggc gataaatacc gcttctggct ttttccagca
    10501 cttcggtgtc gatatcactg gcaaacactt tccagcgtcc gggcgcggtg cccaatgtgt
    10561 cagccagcgt catcgcaatg ctgtacggct cttcgccggt cgaagccgcc gcgctccata
    10621 cgcgatactc gccagaacgg cgacgtgcgt gatccgcgag cagagggaaa tggtgtgcct
    10681 cacggaaaaa tgccgtcaga ttcgtggtca gcgaattgat aaacgcctgc cactcaccgc
    10741 tgtgctgatt agattccagc aagttcagat aatgaccgaa atccgttagt cccagcgaac
    10801 gcaaacgacg aaccagtcgg ttgtaaacca tgtcgcgttt atggtcagcc agaacgatcc
    10861 cggctcgttg atagatcaat tgacttatcc gccgaaaatg cgcgtcggaa agcgccaggc
    10921 gctcggtcat ctgtaacaat aaagacgttt gcccacaggg cagagatgaa gtcatagcgc
    10981 cttctcaatc acttcaggat accactggcg caatttgtaa ctgcgccgac tcatgtcgtg
    11041 ctacttcatg ttcttcaagg gtaaataccg ccacacgcga cgaaagatgg tcggcctgat
    11101 tcgccagttg ttccgttgcc accgccgctt cttctaccag cgaggcgttc tgctgagtca
    11161 cctgatccat ctggctgaca gcctgtgcaa cctgctcaat cccccgctgt tgttcttccg
    11221 acgcagaggc aatttctccc ataatgtcgt tcacgcgggt caccgaactg acaatatcga
    11281 tcatggtcgc ggcggcgtta ttcaccagtt tcgaaccctg ctggacacga ttgactgact
    11341 cttcgatcag ccctttgatc tcttttgctg cctgcgcgct acggctggca agattgcgca
    11401 cttcacctgc cactaccgca aaaccacgcc cctgctctcc ggcgcgagcc gcttccactg
    11461 ccgcattcag ggccagaata ttggtctgga aagcaattcc gtcgataacg ctgataatgt
    11521 cgccaatttt ttgcgagctg gtggcgatct cctgcatggt gtgagtcatg gtactgacct
    11581 gaacaccgcc cgcctgcgcc gttgtcgcgg cattttttgc cagttccgac gcctgtcgtg
    11641 cgttatcggc gttttgccct accgtggcgg ttaattgctc catactggcg gccgtttgtg
    11701 ccagcgatgc cgcctgctgt tcggtacgac ttgagagatc gttattgcct gcgacaatct
    11761 ccgcgatccc aatgtgcatc tcatggcttc ccttacgcac atcactcacc gtcccacgca
    11821 aagcctgctg catggtcttc aggctggcaa aaatggcggt gatctcatta cgaccatata
    11881 ccgcaatcgg acgcgccaga ttacccgcag caatgctgtc aaaatggcta ccaataatgg
    11941 ccagtggttg aacaatcatc ttgcgcgtcc accacagcgc actgctgatg tagattgccg
    12001 caacaataat catgctgata aacaccagcg ccgaaatctg atagttacgc tgactttgcg
    12061 cactggcggc ttccagcaca tggttgatct ccagctgcca ggcctcaaag ttaacgtcaa
    12121 acgcattctg tgatccctgc accggcgcag tgaggaaatc cgaaagttga ttactttcaa
    12181 gccaggtcgc ctgatgttcg agatcgttgt gccagcgggc aaaacttttc tccgtctctt
    12241 tttgcaatgc cctgacgtgc tcgttgcccg cagtcatcgc cataaaactt ttaaacagcg
    12301 tggtggattg cgtcagactg gcgcgcgccg tcgtcatcaa cgttttaatg tcatccgccg
    12361 gatagctaag tgccgtcaga gtgcccgctt tgttcagcgc ggtactggcc tgtaacatta
    12421 ccgcccgagt ttgcgctaat gccgcacgtt gctgattgct ctgctcgacc tgattcagtc
    12481 gttgcaaatc gtcgcgaaat gcccaaaaag acatgccgtt actgccaatc tgcaagatcc
    12541 cgcagagaat caaaattaaa aacagcgtgg tcgaaattcg aatacgatta aacatcaacg
    12601 ctccccatca ggcggcaatg accgcgttag taaatactcg tcaaaatgtt tcccagtttg
    12661 gatcttgttc agcaattcgc agtcgtggct gtgtcggtgg ttgctcactg gcaggacggg
    12721 atggtgtttg cggtttattg gtgagtgggc tggctgccag acggaacgtg gaaaccgctt
    12781 gcgttaaacg actcgcctgt tcttccagcg cagcggcggc ggcagctgat tcctgcacca
    12841 gcgatgcgtt ctgttgcgtg acgcgatcca tttccgaaac cgccaatgcg acttgatcga
    12901 tgccacggct ctgttcatcc gatgccgatg caatctcgcc cataatgtca gtcacgcgag
    12961 tgacagcatt gacgatattg ttcattgttt ccccggcgct ttcgaccagc accgaaccgg
    13021 tatcaacgcg tgagacggag tcttcaatga gggctttgat ctcttttgcc gcctgggcgc
    13081 tacgactggc aagattacgc acttcacccg ccaccacggc aaaaccacgg ccctgttcac
    13141 ccgcacgcgc ggcttcaacc gcggcattca gcgcgaggat attagtctgg aaggcaatac
    13201 cgtcgataac gctgataatg tcggcaattt tcttcgaact atcggcgatc tcatgcatcg
    13261 ttttcactac gccatccacc actttgccgc cgtgctgggc ggtgtcggag gcactttgcg
    13321 ccagttgcga ggcctggcgg gcgttatcgg cgttttgctt cactgtcgcg gtgagctgct
    13381 ccatgctggc ggcagtttct tccagcgcgg atgcctgctg ttcagtacgg gaggaaagat
    13441 cggtgttgcc cgccgcaatt tcacgggtac cggcatagat ggcatctgaa ccttcgcgga
    13501 catgagtgac ggtgtcagtc aaagagcgtt gcatatgtga aacgctctgc gccaggtcgc
    13561 ccatttcact gcgcccgtca atggtcaggg tattcgccag gttaccaccg gcgatttcgc
    13621 gaatgtgagc aataattttt gccagcggag taagcaacat acggcgaatg ccgtaccacg
    13681 ccaccagcag aatcaatacc accaccagcg cgataaccgc cagttgccac tgggcaaatc
    13741 ggtaatcatc tgcgttgtca gtgacgatat cgcgatacag tttttcactg ctgagggcgt
    13801 actgagcaaa cgcttcgccc attgcatttt gcattccctg ggttggctga gcgaaataag
    13861 ctccagtatt gccataatca agataatcaa tcagttcagt taacgctgtg tgatagtttt
    13921 tatatttttc atcaatatta cgactggtag cgaccatttc aggtaacggt gccatgcttt
    13981 tgaatttttt ataatgagtc gcggcctggg ccaatgtttt cctggcgcta tcgagcaatt
    14041 caactttggc gttactttgt tgattggagg aatccatcat catccgcacc gctgaacgac
    14101 tcaggttaat gcgcgtttgc agcattaaat cccaggttga cgtcagctcg ccctgctgtt
    14161 cccgtaattg attggaaacc acaaagctct tctggctatg gtgaagggaa gaaaaaaaca
    14221 gactgccgga aataagctgt aacagtgcga atacccccag caccattacc aacagcgtga
    14281 ctacgcggat acggttaatc ataaggcacc ttcctgaaaa caagttaatc tcgttatcgg
    14341 caagaagggg ggaaacttta ttgctgatgc cacccgccgc gaaattgaaa taaaaaaccc
    14401 gatgcgcaga tcatcggggt catttcaatt gaggaaatcg ggagaattac gccacttctg
    14461 acgccgcgct atctaacagc gccatctctt cgctgttcag cagtttttcg atgttcacca
    14521 gaatcaacat ccggtcgccc agtgcgccca gtccagtgag atattctgtt gaaagcgtca
    14581 cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa tgaaagcacg tctgaaacgc
    14641 cgtcaaccac gatgccgacc acccgctgtc cgagattcag gacgataact accgtgttgt
    14701 cgttatagtc cacatccacc tggctgaact taattcgtaa gtcaacaatc ggcacaataa
    14761 cgccgcgcag attcgtgacg cctttgataa acgctggcgt gttcgcaatc cgtgttacct
    14821 gatcgtagcc acggatctcc tgcactttca ggatatcaat accgtactct tcatcaccaa
    14881 gggtaaatac cagaaattcc tggcctgacg gctcgctggc cagctttgtt acattcgtca
    14941 taccggtcat attgttacct ttttactcat tcaggcggcg gtgttcgcca tacgttgttc
    15001 gcggtttatc gcctgcaagg cggagacatc aacaatcagt gccacgctgc cgtcgccaag
    15061 aatggtcgca gcagaaatgc cggggacttt gcgatagtta ctttcaaggt ttttaaccac
    15121 aacctggtgc tgaccaatta attgatccac cagcaaggcg tagcggcgac cgccactttg
    15181 taagatcacc acaattccct gggtggcttc ggttttcgcg cccgcgacgt tgaacacttt
    15241 ccacagttcg acgatgggca gatattcacc ccgcacttcc agcacccgct cgccgccggc
    15301 cagtggatgc agatcggctt cacggggttg cagtgattcc ataacagcat tcagcggcag
    15361 aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg aggatggcca gcgtcagcgg
    15421 cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac tggatttcga catgaccgcc
    15481 catctcctgg atattacgtt taacgacgtc catgccgacg ccgcgcccgg agacgtcggt
    15541 gacctgctct gccgtggaga agcctggtgc aaatatcagc atcgcgactt cgtcgtcgct
    15601 catgttttcg ctgacagtca aaccttgcga ggccgctttt gccagaattc gctcacggtt
    15661 tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg ccgccctgat gttcggcaga
    15721 cagaattaaa tttccgacgc tgtttttacc tgcggcgagc cgtttttctg gcagttcaat
    15781 accgtgatcg aggctattgc gtaccaggtg ggtcagcggg tcgataatgc gttctatcag
    15841 gcttttgtcg agttcagtag aactgcccac cagcgtcagt tctacctgct tgccgagttt
    15901 tcccgccaga tcacgcacca gccggggata gcgactaaaa acatattcca tcggcatcat
    15961 gcgaatcgac atcactgatt cctgcaaatc acgggcgtta cgttgtaact gccccatgct
    16021 ggttatcaaa tcaccatgat taaccgggtc cagttcgctg gaacgctggg caagcatgga
    16081 ctgggtgata accagctcgc cgacgaggtt aattaattga tcaacctttt ctaccgctac
    16141 acggatgctg gtggattcat tgctgcgcgt cgttttttcc cgctccacgc ggccggttgg
    16201 cgcttgttcg gctgccagtt taagcactgg tggggtggat atttttggcg agacttctac
    16261 tgtttcaaag gtaatctgat cggcttcaat cacaaaacag agtaccgctg tgatgtcatc
    16321 ttcggcgatg tcgcccggta atattgccga gagcgaatcc gcccctttca ccacgtcagt
    16381 taacgttgtc agatgtccca gttcttcttc cagcaggtcg acttccccgg ccttcaggcg
    16441 cgaaaggata attcgtcgcg gcgactgact gcgactctgc tcatcttgcg gttcactttt
    16501 ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg cctttcgctt ctaatgccag
    16561 ttgacgcaag gcctggcaga tataatcgaa gctggcggca tccggctctt gcgactgttt
    16621 ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc aaaaacagat tgataatgtc
    16681 ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc aggttttcca tcagatgcgt
    16741 ggtttcctgc aaaacgctga agccaaaagt tcctgcccct cctttgatcg agtgggcagc
    16801 ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc ggctgcaaaa ccagcagatg
    16861 ctgctccatg tcagccaaca gttcgtccgc ttcatcaaaa aatgtctgat aaaaatcgct
    16921 tatatccatg ctcacgctgt cacctcggtt cggctgatgg cattgtggga acactgacct
    16981 gtggtgcaac ctcaggtttt tccagggcgc ttactggctc attctggctt tcggcgtttt
    17041 catgcaaaat ggcctgttcg gcttgtttgt tcagtaccag caggctgatg cgacggttga
    17101 cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc cgccatgccg acgacacgta
    17161 acactttgcc gctatccaac cctccgacca tcagttcgcg gcgggatgca ttggcccgat
    17221 cggcagaaag ctcccagttg ctatatcctt tctcaccgct ggcgtagggg aaatcatcgg
    17281 tatgacctga aaggctaata cggttgggaa taccgttcag tacaggcgca atggcacgca
    17341 gaatgtcgcg catatagggt tcgacatcgg cactgccggt tctaaacatc gggcgattct
    17401 ggctatcgat gatctgaata cgtagacctt cctggaccag atcgattttg agatggggac
    17461 gtaacgcccg cagtttcgga tcggactcta tcaactggtc gagatcaccc cgcaatttcc
    17521 gcaatcgact ttgctccatg cgttttttca gctcttcgat gttcggctgc ttattcactt
    17581 ccccctggct ttgggtgtaa tcatcaccac cgccgggaat tgggctttca ctattagaaa
    17641 tgcgatcgcc gcccgtaacc gcagtcgcca gtggagtccg gaagtactcc gcaatctgaa
    17701 tcagctcttt tgggctggag atggagatca gccacatcac cagaaaaaag gccatcatcg
    17761 cagtcataaa gtcggcataa gcaatcttcc acgatccatg tgctgccccg tggcttttgg
    17821 ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt cttcatgctt cctcggttgt
    17881 cgtctgttgt tgcggatttt tcaccgcacg cacatgctct tccagttcaa taaacgacgg
    17941 acgttcgctg gaatagagcg ttttgcgacc aaactcaacg gcgataggcg gtgcgtaacc
    18001 gttcagatta gaaagcagag tgactttgac gcactgcatc attttgctgg tttcggcgct
    18061 tttctgacgt aaaacactcg ctaatgggga aataaatccg taagccaata aaatgccgag
    18121 gaaagtcccc accatcgcat gtgcgataag cgcacccagc tcggcggcag gacgatcggc
    18181 tgaacctaac gcgtgaacga cccccattac agccgcgaca ataccaaacg ccggaagtga
    18241 gtccccgacc agcgccagac tgttcgccgg gacttctgcc tcgctttcgt gcgtctcaat
    18301 ctcttcatcc atcagggctt cgatttcgaa ggtgttcata tgaccgctga taatcagacg
    18361 cagataatcg acgataaaat caagcatgac gctatccgcg aggatgcgtg ggtagctggc
    18421 gaagatctcg ctctcacggg gattttcaat atcacgttcc agcgaaaaca tccccatctg
    18481 ccgcgatttc gccatcaacc gataaagcag agccagcaga tccatataca ttgctttggt
    18541 gtatttggag cgacgaaaca gcaacggcag cgccttcagc gtgcctttaa tcgctttgcc
    18601 attattgccg acgataaacg acccaatccc tgcaccggca ataatcacca gttcagcggg
    18661 ttgatagagt gctccaaggc ttccaccggt catcaaataa ccgccgaaaa ctgtaccgag
    18721 aacaaccagg taacctaata agataagcac gacatcatcc ttccactgtt gaccatgaca
    18781 ggatgttcag tcgtcaggcg ttaacgcgcg attggggcaa aaaaaagcag cggtacgtcg
    18841 ttaccgctgc tggaatgttg cgcctcaccg tatcagttaa acagcctgta ctctctgttc
    18901 atccagcagt tgtgggataa tatcggcagg attctgggaa agtttacgtc tttttactgc
    18961 ccgggatggc ggttgacata agctgcaggc aaagctgcca acaggctggt gagcgtgggt
    19021 aataaaattg ccgccgcagc agttgcagct ggaaagttgc agtaatccac tttcaacaaa
    19081 ccgcaccaat gtccaggcac gggttaatgc cagcagtggt ccttcttctg cttgtgggca
    19141 ctgttcaagg tataaacggt aggctttgat caccgcatcg acgccattac acaaaccggt
    19201 tttcagtaaa aactgccatg cattacagaa catcgaagca tgaacgtttt gttcccaggt
    19261 cataaaccag tcggttgaga atggcagcat gcctttcggc ggtgggcttc cgcgcagttc
    19321 tttataaagt tttatcaggc gtccgcgact taactgtgtt tcgctttcca gcatctgcaa
    19381 acgagcgccc agggtgatca attccattgc cagctgaata tcccgcgctt cctgaacaat
    19441 gcttttttca ctcatgatca ggcccttttc ttgcgcagcg cttcttcagg ctgattaaca
    19501 tcattcagca agcgtgttga gagcatgatg ccggtatgaa tttgctggag atcgtcaacg
    19561 cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt caaaacggaa gtgacaaacc
    19621 agttgattgg tttctgccag cttaaccatt tgcggaagag tcagtgccgc taacgttgtc
    19681 gccatttctt catttatgcc gagacgaaac atagcggacg ctttgtcctg aacaatcaaa
    19741 cgctgtgcaa gtagtaaata tgacaagttg atgtcataaa tgtgtttcag caactcggag
    19801 gtatgcatta ttcccaccca gaataaccaa ctttattttt atgcggtttc accgcacccc
    19861 gtgatgtcgc cgggaagccc cggtaaaaaa taattagcat tagaatagtt gcgataagct
    19921 gcaataagca gaaccacctt tttggtttaa tatgtcctta caaatagaaa tgggtcttta
    19981 cacttatcta agatttttcc taaatcgacg caactgtact cgtcactaca cgcacataca
    20041 acggaggggg gctgcgattt tcaataatgt gt
//
LOCUS       DAZFWP020000091        20019 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFWP020000091 DAZFWP020000000
VERSION     DAZFWP020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.091"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(176..847)
                     /gene="queE"
                     /locus_tag="ACHK0Z_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             complement(176..847)
                     /gene="queE"
                     /locus_tag="ACHK0Z_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU4503096.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            986..1126
                     /gene="yqcG"
                     /locus_tag="ACHK0Z_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             986..1126
                     /gene="yqcG"
                     /locus_tag="ACHK0Z_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HGU4503097.1"
                     /translation="MSEENKENEFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1140..2012
                     /locus_tag="ACHK0Z_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /pseudo
     CDS             1140..2012
                     /locus_tag="ACHK0Z_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(2072..3370)
                     /gene="eno"
                     /locus_tag="ACHK0Z_RS15580"
                     /old_locus_tag="pgaptmp_003116"
     CDS             complement(2072..3370)
                     /gene="eno"
                     /locus_tag="ACHK0Z_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU4503098.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3458..5095)
                     /gene="pyrG"
                     /locus_tag="ACHK0Z_RS15585"
                     /old_locus_tag="pgaptmp_003117"
     CDS             complement(3458..5095)
                     /gene="pyrG"
                     /locus_tag="ACHK0Z_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU4503099.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPECKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5323..6114)
                     /gene="mazG"
                     /locus_tag="ACHK0Z_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             complement(5323..6114)
                     /gene="mazG"
                     /locus_tag="ACHK0Z_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU4503100.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6185..6520)
                     /gene="mazF"
                     /locus_tag="ACHK0Z_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             complement(6185..6520)
                     /gene="mazF"
                     /locus_tag="ACHK0Z_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU4503101.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(6520..6768)
                     /gene="mazE"
                     /locus_tag="ACHK0Z_RS15600"
                     /old_locus_tag="pgaptmp_003120"
     CDS             complement(6520..6768)
                     /gene="mazE"
                     /locus_tag="ACHK0Z_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU4503102.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(6846..9080)
                     /gene="relA"
                     /locus_tag="ACHK0Z_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             complement(6846..9080)
                     /gene="relA"
                     /locus_tag="ACHK0Z_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU4503103.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(9128..10429)
                     /gene="rlmD"
                     /locus_tag="ACHK0Z_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             complement(9128..10429)
                     /gene="rlmD"
                     /locus_tag="ACHK0Z_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU4503104.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            10486..13242
                     /gene="barA"
                     /locus_tag="ACHK0Z_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             10486..13242
                     /gene="barA"
                     /locus_tag="ACHK0Z_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU4503105.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(13474..14814)
                     /gene="gudD"
                     /locus_tag="ACHK0Z_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             complement(13474..14814)
                     /gene="gudD"
                     /locus_tag="ACHK0Z_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU4503106.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14835..16175)
                     /locus_tag="ACHK0Z_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             complement(14835..16175)
                     /locus_tag="ACHK0Z_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGU4503107.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(16177..17529)
                     /gene="gudP"
                     /locus_tag="ACHK0Z_RS15630"
                     /old_locus_tag="pgaptmp_003126"
     CDS             complement(16177..17529)
                     /gene="gudP"
                     /locus_tag="ACHK0Z_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU4503108.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSSFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17964..18413)
                     /locus_tag="ACHK0Z_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             complement(17964..18413)
                     /locus_tag="ACHK0Z_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU4503109.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(18431..19213)
                     /gene="truC"
                     /locus_tag="ACHK0Z_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     CDS             complement(18431..19213)
                     /gene="truC"
                     /locus_tag="ACHK0Z_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU4503110.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(19213..19542)
                     /locus_tag="ACHK0Z_RS15645"
                     /old_locus_tag="pgaptmp_003129"
     CDS             complement(19213..19542)
                     /locus_tag="ACHK0Z_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU4503111.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT         4949 a         5366 c         4708 g         4996 t
ORIGIN      
        1 actttaccgc aataattttc actccagcga aaaattaatg ccacagaatt tgtagagaat
       61 gctaatgatt agccctggat gggtaaaatg gaggagtttt cagaggcgac aaacaatata
      121 aatgagtaga agtctttaca ggttccaccc atttataacg cttataaatg tttaatcagg
      181 caatatttag atatttatgt gtttgcatcg acaaacgcca attacgcgca atgcaggttt
      241 caatgcacaa acgtgtggca tcatcctttt ggctaatcgg ctgcagtgca atgactcgcg
      301 gtttatcatc ggtcagcgtt gccagtagtt catccagtgc ttcaatatca cgtacgcgcc
      361 ccactggatg cttgatttcg ttggctcgct ccagtgcctg tgacaacacg tcatagccgc
      421 cgcgcatgtt cagctttggc gatacggtaa cccaggtatt cggtgtgcag cgtacttcat
      481 gagttccact ggtttcgatc tggcagctaa aaccgttctt ttcgagcagg tcagtcagtg
      541 gcagcaaatc atgaatgcaa ggctcaccac ccgtaatcac cacatgccgc gcggtatatc
      601 cctgccgacc aatgacagcc agcaaatctt cactgctcgc cgccccccac ttatcactct
      661 ctttggtctt cgccagaatg ctgaaaaggg agacttcccg atcctcaagc ttttcccagg
      721 tgtgtttggt gtcgcaccag gcacagccaa ccgggcatcc ctgtaaacga ataaaaatgg
      781 cggggacgcc ggtaaagtaa ccctcacctt gcagggtctg gaacatctcg ttaatcgggt
      841 actgcatagc attctctgtg aagtggataa ttgttaatta ttgcagatcc tgccgcaaca
      901 atcatgtctt attaacattc tgttacaggc agatttaaag aaaaatgcga aacatatcgt
      961 taataattaa agggagtaac gtattatgtc agaagaaaat aaagaaaatg aatttaatca
     1021 tgtcaaaaca ttcaccaaaa ttatatttat tttttctgta ttggttttta atgataacga
     1081 atctaaaatt accgatgccg ccgtcaattt atttatccag atttaatgag aaatttataa
     1141 tgcgctattt catactaatg ttcactttcg tatgttcctt tgttgcagcc caaccaacta
     1201 ttgtcccaca attacagtaa caagttacgg atcttacgag tagcttaaac tcacaagaaa
     1261 agaaagaact gacgcacaag ttagaatcta ttttcaataa tacccaagtg caaattgctg
     1321 tattaattgt tcccacaacc aaagacgaga caattgaaca atatgctaca agagtttttg
     1381 acaattggcg tttaggagat gccaaacgta atgatgggat actgatcatt gttgcctggt
     1441 cagatcgtac tgtccgcatc caggtaggtt atgggctgga agaaaaggta accgatgctc
     1501 tggctagaga tatcatccgt agcaacatga tacccgcatt taaacaacaa aaatttgctc
     1561 agggattaga gctagctata aacgctttga ataaccaact cacttcacaa caccaatatc
     1621 cgactaatcc ttcagaaagt gaatcagcgt cttccagtga tcattattac tttgctattt
     1681 tttgggtatt tgcagtgatg ttcttccctt tctggttttt tcatcaaggc agtaattttt
     1741 gtcgcgcatg taaaagtgga gtctatattt cagcaatcta tcttttagat ttattcctgt
     1801 tctccgataa aattttttcc attgctgtat tttccttctt ttttactttc accatattta
     1861 tggtctttac ctgtttatgt gtacttcaga aaagagcatc aggtaaaagt tatcattcag
     1921 acaacagcgg ttccggtgga ggctcaagtg gcggcggatt tagcggcggt ggcggttctt
     1981 ctggaggtgg cggcgcatcc ggccgctggt aactcaacca taaaaaatgc cagcccggag
     2041 gctggcattt ttaaatcaga taaagtcagt cttatgcctg gcctttgatc tctttacgac
     2101 cgttgtacgg tgctttttcg cccagagctt cttcgatacg aatcagctgg ttgtatttag
     2161 caacgcggtc agaacggctc atagaaccag ttttgatctg gcctgcagca gtaccaacag
     2221 ccaggtcagc gatggtagcg tcttcagttt cgccagaacg gtgagagata actgcagtgt
     2281 agccagcatc tttcgccatc ttgattgcag ccagagtttc ggtcagagaa ccgatctggt
     2341 tgaatttgat caggatggag ttagcgatac ctttttcgat accttctttc aggatcttgg
     2401 tgttggttac gaacaggtcg tcaccaacca gctggatttt gtcgcccaga actttggtct
     2461 ggtatgcgaa accgtcccag tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt
     2521 actgtttggt cagttcttcc aggaagtgag tgaattcttc agaggtgaac gctttgttgc
     2581 cttcgccagc cagaacgtat ttaccgtctt tgtagaattc agaagctgcg cagtccatcg
     2641 ccagagtgat gtctttgccc agttcataac cagcagcttt aacagcttca gcgataacag
     2701 ccagagcttc agcgttggaa cccaggttcg gcgcatagcc accttcgtca ccaacagcag
     2761 tgttcatgcc tttcgctttc agaacttttg ccaggtgatg gaaaacttca gaacccatgc
     2821 ggatggcttc tttcacagtt ttcgcgccaa ccggctgaat catgaattcc tggatatcaa
     2881 cgttgttgtc agcgtgctca ccaccgttga tgatgttcat catcggaacc ggcatagagt
     2941 atttgcccgg agtaccgttc agttcagcga tgtgctcgta cagcggcatc cctttagcag
     3001 ctgcagcagc tttggcgtta gccagagata cagccaggat tgcgttcgcg ccgaatttgg
     3061 atttgttttc ggtgccgtcc aggtcgatca tgatcttgtc aatgccagcc tgatctttag
     3121 catctttgcc aatcagcgcc tgagcgatcg ggccgtttac cgcagcaaca gctttggtta
     3181 cgcctttacc caggaaacgg gatttgtcgc catcgcgcag ttccagagct tcacgggaac
     3241 cagtagaagc acctgacgga gcagctgcca taccgacgaa accaccctcc agatgtactt
     3301 cggcttcaac agtcgggtta ccacgggagt cgatgatttc acgaccgatg atttttacga
     3361 ttttggacat taggttttcc tcaagtcact agttaaactg aaactccaga caaacaacgc
     3421 gtacccaggg tacgcgttgc cgttctaact tttttactta cttcgcctga cgtttctgga
     3481 actcgctggc ggctttcaca aagcctgcaa acagcgggtg accatcacgt ggagtagaag
     3541 taaactccgg atggaactgg caagccacga accacgggtg attcggaact tcgatgatct
     3601 cgaccaactg atcatccccg gaacggcccg caacgcgcag acctgcatct tcaatctgtt
     3661 tcaacagcat gttgttgact tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat
     3721 tgtacagctg gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca
     3781 tggttccgcc gagatcgctc ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc
     3841 actcggtaat cagcgccaca accgggtact tacattctgg cacaaattcc gtagagttgg
     3901 cgttctccat gttggcaaca tggcgagcgt aatcaattaa cgccacctgc atacccaggc
     3961 aaatgcccag ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt
     4021 ctacgccacg atagccaaaa ccgccaggta cgaggattgc gtccagacct ttaaggattt
     4081 caacgccgcg cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg ctgacacgat
     4141 tcttcagccc accgtgtttc agtgcttcga tcactgattt ataagcatcc ggcagttcaa
     4201 tgtacttgcc gaccataccg atggtgactt cacttaccgg attcgcttct tcgaagataa
     4261 cctgttccca ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat
     4321 aatcgtccag cccctgagat ttcaacaggc ccggaatttt atagatggaa tcgacgtctt
     4381 tcagagaaat aaccgctttt tccggaacat tacagaacaa tgcaatcttc gcacgttcgt
     4441 tcgccggaac agcgcgatct gaacgacaaa tcaggatgtc aggctggata ccgatggaga
     4501 gcagctcttt tacagagtgc tgagtcggtt tggttttgac ttcaccagac gctgccatgt
     4561 acggcaccag cgtcaggtgc ataaacagag tgtgctcacg gccaatttca acagccatct
     4621 ggcgaatcgc ttcgaggaac ggcaaggatt cgatatcacc tactgtaccg ccgatttcta
     4681 ccagtactac gtcatgacct tcgccacctt ccagcacgcg ctctttgatt gcgttagtga
     4741 tgtgcggaat aacctgcacg gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa
     4801 cgtcagagta gatacgaccc gtggtgaagt tgttgcggcg gctcattttg gtacgaatga
     4861 aacgctcgta gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta acgaacactt
     4921 ccccgtgttg gattgggctc atagtacctg gatcgacgtt gatgtacgga tccagtttca
     4981 tgatggtcac attgaggcca cgggcttcaa gaatggctgc gagggaggct gcggcaatgc
     5041 ctttacccag agaggatacg accccgccgg tcacaaaaat atagttcgtt gtcatgctga
     5101 acctgagaag ttaggttgaa aagacgatgg aataaccagg acgggaaagc agtatacccg
     5161 aacatgacct gtgccacaaa ctttcattat ccctcctctt cgccagcgca ctattgaaat
     5221 caggagtgag aaaatagccc ctttggggta aatgtttttg acgcaaatca agcgcttgtc
     5281 atttaaaaaa tgacacaaat ggcgcttgac cgcgtaattc ccttagagat caatttcctg
     5341 ccgttttacc tgttgccaga cttcttccat tgtttcgagg tcaacacctg tcatttccag
     5401 tccacgcgcg gcaacaatac gctccacttc gcgaaaacga cgctcaaatt tttcgttcgc
     5461 tttttgcaat gcgatttccg cttttgtccc taaatgacga gccagattga ccgtggcaaa
     5521 cagcaggtcc cccatttcct cctccagttt agcctggtcg acaacagcct gccgcgcttc
     5581 gtacatcacc tcgtcgatct cttcgtagac tttatcgact accggaccaa gcgtcgtcca
     5641 atcgaagcca acgttggcgc aacgtttctg gattttttgc gcacgcatta aagccggtaa
     5701 actacgagga atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt
     5761 gatttgctcc caacgggcaa gcacttcact actgttttcg gcagaactat cagcaaaaac
     5821 atgcggatgg cgacgctcta atttatcgct aatagcagcg caaatatcat taaagtcaaa
     5881 gcgcccttct tcctgagcca tttgcgcgta aaacaccacc tggaatagca gatcgcccag
     5941 ttcaccgcga agatcgtcaa aatcttcacg ggcgatggcg tccagcactt cgtaggtttc
     6001 ttcaagggtg taaggcgcaa tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt
     6061 ttccggatcg cgcaggcgct gcataatagt gagcaaacgg tcgatttgat tcattgaatt
     6121 gtcctgaaaa ttgcgggtct gtcaggtgga aacctgtgac cagaatagaa gtgagttagt
     6181 aacactaccc aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa
     6241 ctgttccttt cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta
     6301 acgctacgcc atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt
     6361 gcgttgtata aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca
     6421 ggacaacagc tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc
     6481 aaatcagatc gcccatatcg ggtacgtatc ggcttaccat taccagactt ccttatcttt
     6541 cggctctccc cagtcgatat tctcgtggag gttttccggc gtgatgtcgt tgaccagttc
     6601 agcaagcgta aatacgggct ctttacgcac tggctcaata attaatttgc catccaccag
     6661 gtcaatcttc acttcatcat caatattcag attgagcgcc tgcattaacg tagccgggat
     6721 ccgcaccgcc ggtgaatttc cccaacgctt tacgctactg tggatcataa ccctttcctc
     6781 aaaccgctat catatgtaga tacagtatat atcaatctac attgtagata cgagcaaatt
     6841 tcggcctaac tcccgtgcaa ccgacgcgcg tcgataacat ccggcacctg gttgagttta
     6901 cccagcacgc gccccagcac ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc
     6961 agttgctgtt tggtgtcgct acggctggca acgccaagca cgttcacctt ctcgttggcg
     7021 agaatggtcg tgatatcacg taacaaccca ctacgatcat tagccaccac acggaccacc
     7081 agcgaatatc cggcggagta gctctcaccc cataccgcgt caacaatgcg ttctggcgca
     7141 tgggagcgca gttccgccag ttgttcgcaa tcggcgcggt gtactgaaat accgcgcccc
     7201 tgggtaatga agccgacaat ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg
     7261 tgcatcaggt tgccaacacc ttcgaccacc acgcgaccgt tatctttact gcggttttgc
     7321 ggcgtgtagc ttttttgctg aagttgcttc agcgcggcgg cgtcctgctc ttcggcactc
     7381 ggcttattaa attgcgattg caggaagttc accatctgat tgagacggat atccccgcca
     7441 ccaatcgccg ccagcaactc gtcgacatca ttaaagttgt aacgcggcag cagatgtttt
     7501 tctgcttctt tcaggctgat ccccagatgt tccagctcgt cgtcaaggat ttgccgccca
     7561 gccagaatgt ttttgtcacg gtcctgttta cggaaccagg cgtgaatttt cgaacgccca
     7621 cggctggttg tgacgtaacc gaggtttggg tttaaccagt cacggctggg gttcggctgt
     7681 ttctgggtga taatttcaat ctggtcgccc atctgcagct ggtaggtgaa cggcacaatg
     7741 cgcccgccaa tttttgcccc gatgcagcgg tgtccgacat cactgtggat gtggtaagcg
     7801 aagtccagcg gcgttgatcc cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca
     7861 tacacccggt cgtcaaagac ctggctgcgt acttcgtcga gcatttcgcc ggaatcagcc
     7921 atctcttcct gccacgcaat cagtttacgc agccaggcaa tccggtcttc atgtcccgaa
     7981 cgtgcgccgc cagctgccgc gccctcttta tatttccagt gcgcagcaac acccaactct
     8041 gcatcttcat gcatctgttt ggtgcggatt tggatctcaa cggttttgcc acctggcccc
     8101 agaaccacgg tatgaataga ctgataaccg tttggtttcg ggttagcgac ataatcgtca
     8161 aactcatccg gcaggtggcg atagtgagtg tgcactatcc ccagtgcggc ataacaatcc
     8221 tgtaaacgct cggcgacaat acgtaccgca cgcacatcaa acagctcatc gaaggcgagg
     8281 ttctttttct gcattttgcg ccagatactg tagatgtgtt tcggacgacc atacacttca
     8341 gctttaacgc cttcagcttt catctcagcg cgcagatggc cgacaaactc ttcgatatag
     8401 tgttcgcggt cgagacggcg ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg
     8461 tggagataac ggaagcagta atcttccagc tcccatttca gttgcccaat cccaagacgg
     8521 ttcgccaacg gcgcgtagat attggtgcac tcttttgccg ccagtacgcg ttcatcttcc
     8581 ggcgcatctt ttacttcgcg cagatgagca atacgctccg ccagtttgat gaccacgcag
     8641 cgaaaatcat cgaccatcgc caataacatc cggcgaacgt tatcgacctg ttcggaggaa
     8701 acagaatcag tgtgcgtcgc tttcagctgg cggatcgccg ccatatcacg cacgccgtga
     8761 ataaggttaa cgaccgactt accgacgctc tcacgcagca catcttcgct gactacgttg
     8821 gcatcagcca gagggaacag cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc
     8881 gagaggatct ccaccatctc aacaccacgc cacaataaca gactggcatc cggatgcccc
     8941 tgcgtctgtt gcagacaata cgcccaggtt tcggctaagc actcacacga cttctggctg
     9001 gtaataccca gacttgcgat ccatttttcc ggatcaaatt caccagcctt attgatatgt
     9061 gcacttctta ccgcaaccat tgtcctctcc tttagggacc agacctgccg aaatcggcaa
     9121 atcgtaacta tttaacgcgc gagaaaagta ccatcgattc cagatgtccc gtgtgtggga
     9181 acatatccag catcgccagt cgcgcaatgg tatatcctgc ttttaataac gcttcgctat
     9241 cccgagccag cgttgcaggg ttacaggata cataaactat acgaataggt tccagtttta
     9301 taatttgctg cataacacct gcggcacctg ctcgcgccgg gtccagcaac actttatcga
     9361 agccgttttt cgcccacggc tgctttgtga catcttcttc aagattttcg tgataaaacg
     9421 tcacattctg taaaccatta agacgcgcat tctgctggcc tttttccacc agcgccggaa
     9481 caccttctac accgaccaca ctggctgctt gtttcgccaa tggcagtgta aagttgccca
     9541 taccgcagaa cagatccagt acgcgatctt caggttgcac atccagccat tccaacgcac
     9601 gcgctaccat tttttggttc acacccgcat tgacctgaat aaaatcgcgc gggctaaaag
     9661 ttaagcgcaa cccgtttgag tcataccagg gcatctcacc agagacggtt tcgagtatct
     9721 cactatcggg ggcgagatac agatccaggc cttcagaatg cgaaaagcgt tccagttttt
     9781 cgcgatctgc cgaacttagc ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg
     9841 cctgtaccag ttcaacatga ccaagatggc gcataacttg taagctgccc aggcatgccc
     9901 tgactttggg cagcaatgct tcaagttggg gcactaaaat ggggcattgt ttaacgtcga
     9961 caatgtcact ggagcccgct ttgcgaaacc ccatctgaag ttgctgtgtt ttcggtaagt
    10021 agttcagact taaacgcgcg cggcggcgat agccccaggg aacatcggcg atcacttcag
    10081 agacatcgtg tttcattaat cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca
    10141 cgctggcgtg ttgttgctga cagccgccgc atacgccaaa atgaggacag cgtggtgttt
    10201 cgcgttccgg gctatcgctt aaccggcgta cgactttagc gcgggcatac tgttttttat
    10261 cttcagtaac agcaacttcc gcgttttcct gcggcaataa tccggggata aataacgttt
    10321 tgccgttatg tcgcgccacg ccctgaccaa aagagtcgag gtcgttgact gaaacggtta
    10381 tgatctgacg cgtcgtcgtg cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt
    10441 tctcaattta acagtgtgac cttaattgtc ccataacgga actccatgac caactacagc
    10501 ctgcgcgcac gcatgatgat tctgatcctg gcaccgaccg tccttattgg tttattgctg
    10561 agtatctttt tcgtcgtgca tcgctataac gacttgcagc gtcaactgga agatgccggt
    10621 gccagcatta ttgagccgct tgcagtttct actgaatatg gcatgagcct gcaaaatcgc
    10681 gaatctatcg gtcagttaat aagcgtactg catcgtcgcc attccgatat tgttcgcgcg
    10741 atttcggttt atgatgaaaa taaccgactc tttgtcacct ccaattttca tcttgatccc
    10801 tcatcaatgc agctcggcag caacgtgccg tttcctcgcc agctcactgt cactcgtgac
    10861 ggcgatatta tgatcctccg cacgccgatt atttctgaga gttactcccc cgacgaatcg
    10921 cccagtagcg atgccaaaaa tagtcaaaat atgttgggat atattgcgct ggagctggat
    10981 cttaaatcgg ttcgcttgca gcaatataaa gagatcttta tttccagcgt gatgatgctg
    11041 ttttgtatcg gtattgcgct tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg
    11101 attcgcaaca tggtgaatac cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg
    11161 gaaggattta tgctcggcga gctggatatg ctgaaaaacg gtatcaactc gatggcaatg
    11221 tcgctggctg cttatcacga agagatgcag cacaatatcg accaggcgac gtccgatctg
    11281 cgtgaaacgc tggagcagat ggaaattcag aacgttgagt tagatctggc gaaaaagcgc
    11341 gcccaggaag cggcgcgtat taaatccgag tttctggcaa atatgtcaca cgagctgcgt
    11401 acaccactga atggtgttat tggctttacc cgcctgacgc tgaaaacaga attaacacca
    11461 acgcagcgcg atcacctgaa tacgattgaa cgttcggcaa ataatttgct ggcaattatt
    11521 aatgatgttc tcgacttctc gaaactggaa gcaggtaagc tgattctgga aagtattcca
    11581 ttcccactac gcagcacgct ggatgaagtc gttactctgc tggcacattc ttctcacgat
    11641 aaagggttgg aactgacgct caatattaaa agcgacgtgc ctgataacgt gatcggcgac
    11701 ccactgcgat tacagcaaat catcactaac ctggtgggga atgcaattaa attcaccgag
    11761 aatggcaaca ttgatattct ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt
    11821 gaagtgcaga ttcgggatac cggcattggt attcctgaac gcgatcaatc gcgcttattc
    11881 caggccttcc gacaggctga tgccagtatt tcccgccgtc atggtggcac cggtctgggg
    11941 ctggtgatta cacaaaaact ggttaatgaa atgggcggcg atatttcgtt ccatagccag
    12001 ccgaatcgcg gttcaacttt ctggttccac attaatctcg atctgaaccc gaacattatt
    12061 atcgaagggc catccaccca gtgcctcgcc ggtaagcgcc tggcctatgt cgaaccaaac
    12121 tccgcagcag cgcaatgcac gctggatatt ttaagtgaaa cgccgctgga agtggtttat
    12181 agcccaacgt tctccgcgct gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg
    12241 gtgaccttcc gcgagccgct aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg
    12301 accgatttcc tgatgctggc acttccttgc catgcacaag tcaatgcaga aaaactcaag
    12361 caagatggta tcggcgcgtg tctgctgaaa ccattaacac ctacgcgcct gttgcctgcc
    12421 ctgacggaat tttgtcatca caaacaaaac acgcttttgc ctgtaaccga tgaaagcaag
    12481 ctggcaatga cagtcatggc agttgatgac aaccccgcta acctgaaact tatcggcgca
    12541 ttgctggaag atatggtgca acatgtggaa ctttgcgata gcgggcatca ggcggttgaa
    12601 cgggcgaaac agatgccgtt cgatttgatc ttaatggata ttcaaatgcc tgacatggat
    12661 ggcattcggg cctgcgagct catccaccag ctcccccatc agcaacaaac gccggttatc
    12721 gcggtaacgg cgcatgcaat ggccgggcaa aaagagaagc tgcttggcgc agggatgagc
    12781 gattatctgg cgaaaccgat tgaagaagag cgattgcata atttgttgtt gcgctacaag
    12841 cctggcagcg gtatttcctc tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac
    12901 ccgaatgcga ccctcgactg gcaactggca ctacgccagg cagcaggaaa aaccgattta
    12961 gcgcgcgata tgctgcaaat gttactcgat ttcctgcctg aagttcgcaa caaagttgag
    13021 gaacagctgg ttggagaaaa cccggaaggc ctggttgatt tgattcataa actgcatggc
    13081 agttgcggct atagcggtgt gccgcgtatg aagaatctct gccaactgat cgaacaacag
    13141 ctacgtagcg gtactaaaga agaagatttg gagccggagc tgctggaact gttggacgag
    13201 atggataatg tagcgcgcga agccagcaaa attctcgggt aacggacgtc ttgtcgagta
    13261 gcaatcggct ttttatgacg ctggtttcag attggtgaca aagtgcctgt gtttatgccg
    13321 gatgcggcgt taacgcctta tccggcctac aaaaccaatt aaattcaatg aattaaaaaa
    13381 tgatgtagac ctgatacata gcacaacagg ctattttgcg tttagcatca gtctcaagcc
    13441 ggctccagat agagccggtt ttggttttct gtcttaacgc accatgcacg ggcgcttgtt
    13501 atcgaacgtc cagccaggaa tcagatactg cattcccatc gcatcgtcac gcgcgccaag
    13561 cccgtgtttc tgatacagct catgggcttt catcacttga tccatatcga tttctacacc
    13621 cagccccggt ttttctggca cctgtaccag cccgcctttg atctcaaacg gttctttggt
    13681 caggcgctga ttaccttcct gccagatcca gtgcgtatca atagcagtaa ttttacccgg
    13741 tgcagcggcg gcaacatggg taaacatcgc cagggaaata tcgaagtggt tgtttgagtg
    13801 tgaaccccag gtcaggccaa attcatggca catttgcgcc acacgtaccg aaccttgcat
    13861 cgtccagaaa tgcggatccg ccagcgggat atcaacggat tgcagggaga gcgtatggcc
    13921 catctgccgc cagtcggtgg cgatcatatt ggttgcggtc ggcaggccag ttgcgcgacg
    13981 gaactctgcc atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc
    14041 ataagccagc gaccctttca ggtatttacc aattttaatc gcttcgttca gcgaccaggc
    14101 accgttagga tcgagcgtaa tacgcgcctg cgggaagcgt tgcgccagtg ccacaataga
    14161 ctcggcctct tcttccccgg ccagtacacc gcctttcagt ttgaaatcgt tgaagccata
    14221 tttttcatac gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg
    14281 acgcaggcga taccagtcgc atgagtcatc cggctggctt tgatacggca gcggtgtggc
    14341 tttgcgatca ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttgacc
    14401 atcgcccagc agggatgcca cgtttacccc cagatgctgc cccagcagat ccagcattgc
    14461 cgcttctatc ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg
    14521 cccaccagca tcacgatcgg caaaagtatt acgcaccagc gtcagaacgt ttttgtattc
    14581 acccagcgtt ttacctacca ccagcggaat cgcatcttcc agcgttttac ggattttctc
    14641 gccgccggga atttccccta cgccagtgtg accagaatta tctttgataa tcacaatatt
    14701 acgcgtaaag aacggtgcgt gtgcaccact cagattcatc agcatactgt catgacccgc
    14761 caccgggatg acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat
    14821 gtcctttttc agaatcaatg acggccgaaa acgggacgtt tacggtcaaa ggtccagccg
    14881 gggatcaggt actgcatcgg acctgcgtcg ttacgcgcac cgacaggcag acgtttatag
    14941 gcctcatgtg ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca
    15001 ggaacggcaa tttttccgtt tttaatctcc agcggatttt tggtcaggcg acaatcgccc
    15061 tcctgccaaa tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcgcccaca
    15121 tgggtaaaca tcgccagaga gatatcgaaa tggttattag aatggcagcc ccaggtcagc
    15181 ccccagtcgt cgcaaagctg cgccacacgg actgcaccgg aaagcgtcca gaagtgcgga
    15241 tcggcaagtg gaatatctac cgcattgagc atcaccgcat gacccatttc gcgccagttg
    15301 gtggcgatca tgttagtcgc gacgggcaag ccggtcgccc gccgaaactc cgccataact
    15361 tcacgaccgg agaaaccctg ttctgcgccg catggatctt cggcataggt aagaacatca
    15421 ttcagccctt tgcatagaga aatggcttca tcaagtagcc aggcaccgtt gggatcaacg
    15481 gtaatccgcg catcagggaa gcgtttcttc aatgcacgaa cagtgtcgat ttcttgctcg
    15541 ccaggtaaca ccccgccctt aagtttgaaa tctttaaagc catagcgatc ctgtgaggct
    15601 tccgccagac gcacaacggc ttcgctgttc atcgctttct gatggcgcaa ctgataccac
    15661 tcatggttgc ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcaccgata
    15721 taaaacagat aaccgaggac ggtaatagtc tcgcgttgct tgcctggccc caacagttcg
    15781 cagaccggaa cattcagcgc cttacctagc aggtcaagca aggcggcttc cagcgccgcc
    15841 acggcattaa cgcgcaattc aaaagtccag gcacctttgc cgaaggtatc aaaatcggct
    15901 gcctgattac ctttatgcac ctgctgaacc actttattca ggcgcgcaac ttcctggccc
    15961 agaaccatcg gaatagcatc gaccagtgtt tgataaatca cctctccgcc cggcgcttca
    16021 ccaatgccgg tatgcccggc gttatcggtg agtaccacaa tattgcgggt gaaatatgcg
    16081 ttatgtgccc caccaatatt aagcaacatg ctatcgtgcc cggcgaccgg aatgactttc
    16141 atatcagtaa taacaggact ggattgtgtc gtcatcttat tgccccgcga caggtttcag
    16201 ctcgatacgc ttgatatcgc ccaccagcac caggtagctc agtaccgcga ttaaggcatg
    16261 aacaccaaca taaatcagcg ccccattaaa ggagccagtc gtgccaacga tataaccaat
    16321 tgcgattggc gtgacgatgc cagaaatgtt accgaacatg ttgaacaggc caccggaaag
    16381 accgctgatc tcttttggcg cggtatctgc cattactgcc caacccagcg ccccgatgcc
    16441 cttaccgaag aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt
    16501 gcagaacacc atcaccatcg acagcaacat gcccattacg atcggtgttt tacgcgcaat
    16561 gttcagcgat cccgtgcggc gcatcagcca gtcggaaata atcccaccca gcacaccgcc
    16621 gataaaaccg caaaccgccg gaacggaagc cacaaagccc gctttcagaa tcgacatccc
    16681 acgcgcctgc accagataaa ccggaaacca ggtaatgaaa aagtaagtca gggcgttgat
    16741 acagtactga ccgatataaa cgccgatcat catccgtgac ccgagcagtt gtttgatctg
    16801 cccccacttc acgctgaacg gcactttaac tttggtgttt tgctgatcca tattgatcag
    16861 cgcgccaccc gcggcgatgt actccagctc tttctgattt acccccggat gttgatttgg
    16921 ctcgtggatg actttcaacc agataaagct gatgacaatc cccagaccgc ccataaagaa
    16981 gaagacgtgt gaccagccca cttcatgcgt cagccagccc ataatcggcg cgaagatcac
    17041 cgttgcgaag tattgagcgg agttaaaaat cgacaccgcc gttccccttt cctgcgccgg
    17101 aaaccaggcc gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag caagcccgac
    17161 caggaagcgc agagtaaaca gggcaacgat aatgccgaat ccactaaaga tatcgacgaa
    17221 gccttgcagc aaggtaaaca tcgaccagat aaagctcgac cagaagtaga cgcgttttga
    17281 accaaaacga tccagcaacc aaccaccagg gatctgcccg ataacataag cccatgagaa
    17341 ggcagagaac acatagccca ttcccacggg atcaaggccg atatctttgg ccatttccga
    17401 accggcaata gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat
    17461 caccactatc cagtaacgag cattcgtgcg tttttccaca ctgctcgcag cctgacttaa
    17521 agaactcatt gttgcactcc tgaaaattcg cgttagccac gctcactctg gactgcgaca
    17581 tagccaggaa atcagaggtg acgtagggtg ttttttgccg tttttatagg tcgttcgccg
    17641 aatacggcgc gtgtttatat ctggcaatag cagtataaaa agcgcgccat agcggctcac
    17701 cgtgcaacaa cacaacatta atgcgttcaa tgaggcccga ttttggcatt agccctggac
    17761 ggtggaatcc acttcacgga aatgaaaaca agaacaagaa aggaagggtt aaaacgaaga
    17821 aataaagaag agtatgaaat ggatcgcttg actccaggca aacgccagta aaaatccgcg
    17881 ctatgaagca gtttttactg gcatttgcct gaaaatattc gattcagcac cgctaaaacg
    17941 acatttaccg ctcgctgaac atatcaggac aacagcgtgc cccactgttc gacccacgga
    18001 tttgattccg tttccggttc cgggttctca ctggcgtcaa tcaacagcat ttcgccaacc
    18061 cgctgagcgc tctgttcctg caacaaggca tcgaactgtt tgccgccatt gcagaaattt
    18121 acataactgc tgtcgccgag cgcaatcacg ccataacgca gattcggctg gaagcccaga
    18181 ctatctttga ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc ctgcccggtc
    18241 gtggacgtaa ccaccagaac atacttatcc tgatagggca gccagtcgct taattcagga
    18301 tcttcaaata ccgttgcttt gtggccctgt gcggtcagaa ttgcttctgc ttcttccgcc
    18361 actaacagcg aatttccgta catggtgccg acaaaaatac caatttccgc cataccgttt
    18421 tctccctgga ttaggaactt atctctccat cctgacccga tggcgcactg aactcaaccc
    18481 tttcattttc aggaagcaga ccgcgccagc caaattgtga taacgcctgc atccaggtgt
    18541 cgtccaggcc cgcgtgaata gtcagcggct cgccagtaaa aggatgcgtc aatgacaact
    18601 gactggcatg tagcattaac cgctggaggc caaaatgctc agcaccgctg cgattctggc
    18661 gtaaatcgcc atgtttgcta tcgccaataa tcggatgacg caaatgggca agatgtcggc
    18721 gaagctgatg tttgcgtccg gttttcggct ccagttccac caggccgtag cgcgtggtcg
    18781 ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag accgcgataa tgcgtcactg
    18841 ctggctgcga gcctttatct tcgcgggcaa atttatcagc gattttgtcc agttcttcca
    18901 ccagtggata atccagaact gcttcttcca tcaaccagcc gcgcacaatc gcatggtaac
    18961 gtttctggat ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc tcgctggata
    19021 atcccatcaa caacacacca gaagtgggtc ggtccagacg atgagcggta aaaacgtgct
    19081 ggccaatctg atcacgcacg gtttgcatga ccactacttt ctcgtcgcga tccagccagc
    19141 tgcggtgaac cagccagccg gagggtttat ttaccgcaac cagccattca tcctgataga
    19201 gtatttccag cattagctcg catcatccgc aaaaagagca tccagttttt ccagctcagc
    19261 cagaataagc gcgcgttgcg gatggtccgt cgccagcgcc atttcataat agggtgcaac
    19321 ggcaaaagcg cccggtaacg gctgtttgtt atctaacaaa tcgtgcattc gcgggatcag
    19381 cacccactgc aaccactcca gtggttccat ggtgtccata aagaacggtt gggtactatt
    19441 aaattgatgc ggctggggtt catcgtttcg ccagtgctga tgttcacgca gtaacgcttc
    19501 aagcgcctgc aactggagac gaacgcggtc atgagtggtc ataaagcaac ctcaataaga
    19561 aaaactgccg cgaaggatag caggaataaa aaaagggagc actgtattca cagcgctccc
    19621 ggttcgtttc gcagcattcc agctactttt gttgctccct gctcatcctt gacaactttt
    19681 cctctggcct tgcggccaat cgttcatcct gaactattgc ttcctgctca caccaccccg
    19741 atgtgatact tcatcctgaa gtgtccctgg ccttcctgac ccaccgaatc atcctgaccg
    19801 gttctcattc tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc tcttcgcttc
    19861 atccagaagc ctttccctga aacaccatcc tggtgtgtcc tgcagaagtg tcatcatcct
    19921 gatgttcact tcgttgtctg actccctgtc gacgaagata gaatcgtctt tttcgcgaag
    19981 tcttacaagg cgcttacaga caatgcctta aggaaaatt
//
LOCUS       DAZFWP020000092        19598 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFWP020000092 DAZFWP020000000
VERSION     DAZFWP020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.092"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(109..1329)
                     /gene="fsr"
                     /locus_tag="ACHK0Z_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             complement(109..1329)
                     /gene="fsr"
                     /locus_tag="ACHK0Z_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU4503112.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1547..3199
                     /gene="ushA"
                     /locus_tag="ACHK0Z_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             1547..3199
                     /gene="ushA"
                     /locus_tag="ACHK0Z_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU4503113.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3236..3715)
                     /gene="ybaK"
                     /locus_tag="ACHK0Z_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             complement(3236..3715)
                     /gene="ybaK"
                     /locus_tag="ACHK0Z_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU4503114.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3919..4713)
                     /gene="ybaP"
                     /locus_tag="ACHK0Z_RS15665"
                     /old_locus_tag="pgaptmp_003133"
     CDS             complement(3919..4713)
                     /gene="ybaP"
                     /locus_tag="ACHK0Z_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU4503115.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4851..5192
                     /gene="ybaQ"
                     /locus_tag="ACHK0Z_RS15670"
                     /old_locus_tag="pgaptmp_003134"
     CDS             4851..5192
                     /gene="ybaQ"
                     /locus_tag="ACHK0Z_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4503116.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5407..7911)
                     /gene="copA"
                     /locus_tag="ACHK0Z_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             complement(5407..7911)
                     /gene="copA"
                     /locus_tag="ACHK0Z_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU4503117.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8173..9105
                     /gene="glsA"
                     /locus_tag="ACHK0Z_RS15680"
                     /old_locus_tag="pgaptmp_003136"
     CDS             8173..9105
                     /gene="glsA"
                     /locus_tag="ACHK0Z_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU4503118.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVANQLGYNVFKG"
     gene            9108..10400
                     /gene="ybaT"
                     /locus_tag="ACHK0Z_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             9108..10400
                     /gene="ybaT"
                     /locus_tag="ACHK0Z_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4503119.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10431..10528
                     /locus_tag="ACHK0Z_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /pseudo
     CDS             10431..10528
                     /locus_tag="ACHK0Z_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10525..10932
                     /gene="cueR"
                     /locus_tag="ACHK0Z_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             10525..10932
                     /gene="cueR"
                     /locus_tag="ACHK0Z_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU4503120.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAESERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10933..11391)
                     /gene="ybbJ"
                     /locus_tag="ACHK0Z_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             complement(10933..11391)
                     /gene="ybbJ"
                     /locus_tag="ACHK0Z_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU4503121.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11388..12305)
                     /gene="qmcA"
                     /locus_tag="ACHK0Z_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             complement(11388..12305)
                     /gene="qmcA"
                     /locus_tag="ACHK0Z_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4503122.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12451..13128
                     /gene="fetA"
                     /locus_tag="ACHK0Z_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             12451..13128
                     /gene="fetA"
                     /locus_tag="ACHK0Z_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU4503123.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            13115..13894
                     /gene="fetB"
                     /locus_tag="ACHK0Z_RS15715"
                     /old_locus_tag="pgaptmp_003143"
     CDS             13115..13894
                     /gene="fetB"
                     /locus_tag="ACHK0Z_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU4503124.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13957..14811)
                     /gene="cnoX"
                     /locus_tag="ACHK0Z_RS15720"
                     /old_locus_tag="pgaptmp_003144"
     CDS             complement(13957..14811)
                     /gene="cnoX"
                     /locus_tag="ACHK0Z_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU4503125.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14872..15681)
                     /gene="ybbO"
                     /locus_tag="ACHK0Z_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             complement(14872..15681)
                     /gene="ybbO"
                     /locus_tag="ACHK0Z_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4503126.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15671..16294)
                     /gene="tesA"
                     /locus_tag="ACHK0Z_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             complement(15671..16294)
                     /gene="tesA"
                     /locus_tag="ACHK0Z_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU4503127.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16265..16951
                     /gene="ybbA"
                     /locus_tag="ACHK0Z_RS15735"
                     /old_locus_tag="pgaptmp_003147"
     CDS             16265..16951
                     /gene="ybbA"
                     /locus_tag="ACHK0Z_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU4503128.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLGHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16948..19362
                     /gene="ybbP"
                     /locus_tag="ACHK0Z_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             16948..19362
                     /gene="ybbP"
                     /locus_tag="ACHK0Z_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU4503129.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLLLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHLELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         4569 a         5091 c         5317 g         4621 t
ORIGIN      
        1 agctgcaaaa aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg
       61 gcgactgtaa aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg
      121 ccggttatca ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt
      181 atagactaac tcgatgctgg tgtgatcggc gataagcccc agaactgccg ctcccagacc
      241 tcccatgcca aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag
      301 tagctcctga gcgtagacca gaatggcaga gaatgccgaa gcgaggataa atccaataat
      361 caccgttaaa acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc
      421 aacgccgagg atagagcccc aaatcacata tttccgccca attttatccc ctacaggccc
      481 gccgatcacc gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgggcatt
      541 ctggatagat aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc
      601 catatagaaa tatttcgaga aaatgaggat taacagaatg ctgactgcca gtaccacttt
      661 gttgcgcggc agtggattga ttatcgtcgc tttggatttt cctttattca ttcggtgctg
      721 tgccgagtac caacggctaa tttgcgccaa caccacgatc gccagcagtg ccgcaagcac
      781 aaaccaggca acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc
      841 cagggaactg ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc
      901 gccggaagcc atacgggcta cgcgagaaga ttccggatga aagaccgatg aaccggtacc
      961 gaccagcgcc gccgccagca gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag
     1021 accacttaag gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt
     1081 ccagtagccg accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat
     1141 gccaatctgc ataaatgtca gagaaaattc tgactgaagc agcggataaa tcgccagaat
     1201 cagcgattgg atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa
     1261 cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg gttgttcact
     1321 cattgccata ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga
     1381 ttattacccg actaacatac ctgtatgcgt cgcctgaagg aagtctcaac gccgaataca
     1441 gaatttctaa tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc
     1501 gtcatactat ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca
     1561 gcggggcgtg gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc
     1621 gtatgagcag gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca
     1681 tttttggcgc aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg
     1741 tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat
     1801 taacactggc gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa
     1861 tctggtgggc tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt
     1921 attacgccag caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa
     1981 aagtactggc gagcgcctgt ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat
     2041 tgccgtcatt gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac
     2101 tgatgtcgaa tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca
     2161 gacagaaaag ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga
     2221 gcacggctct aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct
     2281 ggcgatgatc gtcggtggtc actcgcaaga tccggtctgc atggcggcag aaaacaaaaa
     2341 acaggtcgat tacgtgccgg gtacgccatg caagccagat caacaaaacg gcatctggat
     2401 tgtgcaggcg catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg
     2461 cgaaatgaaa atggttaact accagctgat tccggtgaac ctgaagaaga aagtgacctg
     2521 ggaagacggg aaaagcgagc gcgtgcttta cactcctgaa atcgctgaaa accagcaaat
     2581 gatctcgctg ttatcaccgt tccagaacaa aggcaaagcg cagctggaag tgaaaatagg
     2641 cgaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agaccaatat
     2701 ggggcggttg attctggcag cccaaatgga tcgcactggt gccgactttg cggtgatgag
     2761 cggaggcgga attcgtgatt ctatcgaagc aggcgatatc agctataaaa acgtgctgaa
     2821 agtgcagcca ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga
     2881 ttacctgacc gccgtcgcgc agatgaagcc agattcaggt gcctacccgc aatttgccaa
     2941 cgttagcttt gtggcgaaag acggcaaact gaacgacctt aaaatcaaag gcgaaccggt
     3001 cgatccggcg aaaacttacc gtatggcgac attaaacttc aatgccaccg gcggtgatgg
     3061 atatccgcgc cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt
     3121 gctgaaagcg tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg
     3181 tgaggtgagc tggcagtaat ccgaaagtgc cggatgtttg catccggcac aatgcttaat
     3241 cgcggcgggc gatatcagca aatttggcat cgaggatctt tgccagatcg cctgccgcca
     3301 gttcgatatc cagtccgcgc ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg
     3361 gggcgtcgat aatcgttggc agacgttttt tctgccccag tgggctaatc cccccaacca
     3421 ggtatcccgt cgaacgctgc gcgaccatcg gatcggccat ctcaactttc ttggcaccca
     3481 gcgcttttgc tactttttta agatccagtt gacctgcgac cggcgtaacg gccacggcaa
     3541 ggtgtttcat atcaccgttc actgccacca gcagcgtttt gtagacctga tccggattca
     3601 aacctaattt tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg
     3661 tatggatttg aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa
     3721 ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa
     3781 gagtcatctt gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac
     3841 cgtcgcttaa agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata
     3901 gcgatattgg ccattttttt agcgcaacat ttgcggcaaa ttcccttctc catacaggtg
     3961 tagtgcaccg accgcgacca catatcgccc cggcggcatg gcgcgtaatt tatcccgcca
     4021 ggcgagattt cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattgggcag
     4081 cgttatatca ttattttgcg gcggtgcatt cagccaccag ctcatcattt gttgcagcaa
     4141 ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg
     4201 gagctggagc aacatggcaa tctggttttc agccccttcc agttcaatca cgggtttatg
     4261 ttgttgcttc gccgcctgca ataactggta atcgataccg tattctgccc gcagccccag
     4321 tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gagtagaaaa
     4381 gagcgacgga gaaatgccca tctcctggct aatgtgctgc agattttgga gttgctcctc
     4441 gttaatgcgc tcttccagcg cctcgcaagt aggcaaatta gcaaaaggcg tatcgctggt
     4501 ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt
     4561 gggcagggga gccatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg
     4621 attgccggga agggtgatat cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc
     4681 ccaaagcgtt tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc
     4741 tagcgcgcgg tgagggatgg cgcaaatgga gttggggact tgtttaatgt gtttgtatga
     4801 ttcagtatgt tcttgcatcg ctattcacag ggaagcaaca gttaaaaacc atgaaacagg
     4861 caacaagaaa accgacgaca cccggagata ttcttctcta tgaatatctg gaaccgctcg
     4921 atttgaaaat caatgagtta gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac
     4981 tgatcaataa caatcgtaaa ctcaccactg agatggcatt ccgtctggcg aaagtttttg
     5041 ataccacagt cgattttagg ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa
     5101 acaacatgcg cacccaggaa gaattgggac ggattgaaac agtggctgaa tatttggcac
     5161 gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc
     5221 cggataaggc gttcacgccg catccggcat ccgagcacca ttgcctgatg cgacgcttac
     5281 ccgtcttatc aggcctacaa acctgtgcct gaaccgtagg ccggataagg cgtttacgcc
     5341 gcatccggca gtcatgcgtc gatgccaaat gcgccgccct aaagcagcgc atccgcaatg
     5401 acgtacttat tccttcggtt taaaccgcag caaccggttg gcgttactca ctacggtaat
     5461 cgacgagagc gccattgccg ctccggcaac taccgggtta agcagtgttc cagtaaacgg
     5521 ccacaaaata ccggcggcga ccggaatacc gatgctgttg tagataaagg caccgagcag
     5581 gttctgcttc atgttgcgca gcgttgcgcg ggaaatcgcg agcgcatccg caacgcccat
     5641 caggctatgg cgcatcaggg taatcgccgc ggtttcaatg gcaacatcac tgccgccacc
     5701 catcgcaatg ccgacatccg cctgggccag cgctggcgcg tcgttaatgc cgtcgcccac
     5761 cattgccacc tgacgtcctt cactttgcag gcgtttaatc gcttcggctt taccgtccgg
     5821 cagcaccccg gcgatcactt catcaatccc ggcttctttg gcgatcgcat tggcagtggt
     5881 tgggttatcc ccggtcaaca tcaccagacg atatcccgct ttatgcaggc gttgcagcgc
     5941 cgccacgcta tcactacgca atggatcgcg tactgccagc agggctaccg ctttcccgtc
     6001 aaccgccagc agcacaggcg ttgccccttg cgatgcctga gcagtaatct ccgcttcgat
     6061 agctttggta ccaacctgtt gctcatttaa cagcgcctga ttgcccagca ataacgcatg
     6121 accttcagct tcaccgctca cgcccagccc gcgcaatgtg cggaaaccgt tgacctgcgg
     6181 cagctgcata tcacctgctt tatcgaggat cgcccgtgcc agcggatggc tggaaccttg
     6241 ctccagtgcc gccgccagac gcaatgcctg cgcttcatca acatcagcaa acgttttcac
     6301 tgcgacaacc tgcggcttcc cttcggtcag cgtcccggtt ttatcgaaca cgacggtatc
     6361 gagtgtactg gcgcgttgca gcgcgtcggc gtcccgcacc agcacgccaa actcagccgc
     6421 ccgcccgacg ccggaaataa tcgacatcgg cgtcgccagc cccagcgcac acggacaggc
     6481 gataatcagt accgtggtgg caatcaccag ggtataaaca atttgcggtg ccggaccaaa
     6541 gaagtaccag attgccgcac tgaccagcgc aatcaccacc accacaggta caaatacggc
     6601 tgagatttta tccgccagct gaccgatttc tggcttgctg ctctgggcct ggcgcaccat
     6661 gcgaatgatc cgtgacagcg tggtatggct gccaaccgca ctggcacgaa acagcacgct
     6721 accgtcctgt accactgtcc cggcatggac gctttcgcct tcgccttttt gctgcgggat
     6781 tggttcgccc gtcagcatcg cttcatccag ccatgcttcg ccctgggtaa tctcgccatc
     6841 taccggcacg cgatcgccgg tcgtcaggcg cagcaacata cctggctgca cttccgccag
     6901 aggcacgctt ttttcacctt cgtcagtaac caggcgcgcc gtcggtgggg ttaaatcgag
     6961 taatttctcc agcgccttag aagaacgctg acgtgcgcgt gcttccagca tatggccgag
     7021 attgatcaga ccgataatca tcgcgctggc ttcgtaataa agatgtcgcg cttccatcgg
     7081 gaaccactgc ggccacaggt tgacgctcat cgaatagagc cacgccacgc cagtacccag
     7141 cgccaccagc gtatccatcg tcgctgcacc gttcagcagg cttttccatg cactgcggta
     7201 aaaatggccg ccggcgaaaa ccatcaccgc cagggttatc aggccgataa ccaaccacag
     7261 gctgcggttg tcagcggtga ccatcatgtt atcgccaatc atcccccaga ccatcaccgg
     7321 gatacccacc gccagtgcga caattgcctg ccagcggaag cgcttcatcg tagcaacggc
     7381 ggtttcctgc tggcgctcac ggcgtttcgc gtcatcttca atcgcttccg cgccgtagcc
     7441 cgctttttcc accgcctgca ctaaatcttg tggggaggca ctgcccatca ccagcgcagt
     7501 acgctccgcc aggtttaccc gtgcctgagt gacgcccggt acgctttgca gcgcattttg
     7561 tacgcgggtg acacagctgg cgcagctcat gccgctcagc agcaactgct ggctgtcatc
     7621 gtcatcggcg gtcgctgccg gaagcgcctc agaaaccgct gtcagtgctt ccgacgggat
     7681 tgatgactcc gccagcggtt tagcctttgg gtggcttaca gatgcgtcat aacccgcttg
     7741 tttgatggtt tcgatcagct gttctgcact ggcagtcccg gtaacgtgcg cttcagtgat
     7801 agacacatcc gcttgttcca catccgggcg ctgttcgaga ctttctttca cgcgtttaac
     7861 gcagtgaccg caggacaggc cgtccagggt caggtcgata gtttgtgaca taaaacactc
     7921 ctttaagaca gttttgactg gctgtgataa aggttaaacc ttccagcaag gggaaggtca
     7981 agaaattaat aaaccaggcg agtaaaagtc cgtaaagatt aaaaaattgg ctcaatttgc
     8041 atcaggatta gacatttatc tctttgtttt cctgtagtta agttgcgggt gctaagttaa
     8101 atcaagatgc ctgaaaatcg gcaccggggt gaggaattac ctcccgcatc tataaaaagg
     8161 agttaacaaa agatgttaga tgcaaacaaa ttacagcagg cagtggatca ggcttacacc
     8221 caatttcact cacttaacgg cggacaaaat gccgactaca ttccctttct ggcgaatgta
     8281 cctagtcaac tggcagcagt ggctattgtg acctgcgatg gcaacatcta tcgcgcgggt
     8341 gatagtgatt accgctttgc gctggaatcc atctcgaaag tctgtacgtt agcccttgcg
     8401 ttagaagatg tcggcccgca ggcggtacag gacaaaattg gcgctgaccc gaccggattg
     8461 ccctttaact cagttatcgc cttagagttg catggcggca aaccactgtc gccactggta
     8521 aatgctggcg ccattgccac caccagcctg attaacgctg aaaatgttga acaacgctgg
     8581 cagcgaattt tacatatcca acagcaactg gctggtgagc aggtagcgct ctctgacgaa
     8641 gtcaaccagt cggaacaaac aaccaacttc cataaccggg ccattgcctg gctgctgtac
     8701 tccgccggat atctctattg tgatgcaatg gaagcctgtg acgtgtacac ccgtcagtgc
     8761 tccacgctcc tcaatactgt tgaactggca acacttggcg cgacgctggc ggcggggggt
     8821 gtgaatccgc tgacgcatga acgcgttctt caggccgaca acgtgccgta cattctggcc
     8881 gaaatgatga tggaagggct gtatggtcgc tccggtgact gggcgtatcg cgttggttta
     8941 ccgggcaaaa gcggtgtagg tggcggtatt ctggcggtcg tccctggcgt gatgggaatt
     9001 gccgcgttct caccaccgct ggacgaagaa ggcaacagtg ttcgcggtca aaaaatggtg
     9061 gcatcggtcg ctaatcaact cggctataac gtgtttaagg gctgatcatg atgaacacgg
     9121 aaggtaataa cggtaacaaa cccctcggtc tatggaatgt cgtttccatc ggtattgggg
     9181 caatggtggg ggcggggatc ttcgcgctgc tggggcaggc tgcgttgcta atggaagcct
     9241 cgacctgggt cgcctttgct tttggcggta ttgtggcgat gttttccggt tatgcctatg
     9301 cgcgtctggg ggcgagctat cccagcaatg gcggcattat cgacttcttt cgtcgcggat
     9361 taggcaacgg cgtcttttcg ctggcgctct cgttactgta cctgttgacg ctggcggtga
     9421 gcatcgccat ggtcgcccgt gcttttggcg cttatgccgt gcagtttttg catgaaggca
     9481 gccaggagga gcaccttatt ttgctctacg cgttggggat cattgcggtg atgacgcttt
     9541 tcaactcctt aagcaaccat gcggtagggc ggctggaagt gatcctcgtc ggcattaaaa
     9601 tgatgatcct gttattgctg attattgccg gtgtctggtc gctgcaaccg gcgcatattt
     9661 ccgtctctgc gccccccagc tccggtgcgt tcttctcctg tatcgggata accttccttg
     9721 cctatgcggg ctttggcatg atggcgaacg cggcggataa agtgaaagat ccgcaggtca
     9781 ttatgccgcg ggcgtttctg gtggcgattg gcgttaccac gttgctttat atctcgctgg
     9841 cactggtttt gcttagcgat gtatcggcat tagagttaga aaaatatgcc gataccgccg
     9901 ttgcgcaggc tgcttctccg ctgctcggac atgtgggtta tgtgatcgtc gtcatcggcg
     9961 ctttactggc gacggcttca gccattaacg cgaacctgtt cgccgtgttt aacatcatgg
    10021 acaacatggg cagcgaacgc gaactgccga agctaatgaa taaatccctg tggcggcaga
    10081 gtacctgggg caacatcatt gtcgtggtgt tgattatgct gatgacggcg gcgctaaatt
    10141 taggctcact cgccagtgtt gccagcgcca cctttttgat ttgctacctg gcggtgtttg
    10201 tggtggcgat ccgcctgcgt catgatattc acgcctcgtt gccgattctt atcgttggta
    10261 cgttggtgat gttgttggtg atcgttggct ttatctacag tctgtggtcc cagggtagcc
    10321 gtgcgttgat atggattatt ggctcactct tactcagcct tattgtggca atggtcatga
    10381 agcgcaataa aaccgtataa catctctctg tgcgcagtac ttcctgtatt attgtggtgg
    10441 cggtcgatat tcgcactggc aaaaaaacgt gcttgaatat ctgttgaaac cctttaacaa
    10501 agcacaggag gcgttgcgcg aacgatgaac atcagcgatg tagcaaaaat taccggcctg
    10561 accagcaaag ccattcgctt ctatgaagag aaggggctgg tgacgccgcc gatgcgcagc
    10621 gaaaacggtt atcgcaccta cacgcagcag catctcaacg aactgacctt actgcgccag
    10681 gcacggcagg tgggctttaa cctggaagag agcggcgagc tggtgaatct gtttaacgac
    10741 ccgcagcggc acagcgccga cgtcaaacgg cgcacgctgg agaaggtggc ggagagcgaa
    10801 cgacacattg aggagctgca atccatgcgc gaccagctgc tggcactggc gaatgcctgc
    10861 cctggcgatg acagcgccga ctgcccgatt atcgaaaatc tctccggctg ctgtcatcat
    10921 cgggcagggt gattaagacg agacggctcg gatatgtagg gttatccctt ctatcgcaat
    10981 gacttcaaca tgcgtacctg caccgagatc ctcgctggcg ctgacaggcc atgaactgtc
    11041 accgacgcgc atatgaccgc gcccgttgac cagcggagat tccagcacaa aacgtcggcc
    11101 aatcagctgc tgcccgcgct ggtttaaatg actatcgctg tgcttttgtt cgcgcacccg
    11161 ccgcgacaac catttccacc acagccaggc ggcgagcagc gtcaggatgg caaacattac
    11221 cccttgccac tcccaaccca gcggcaccag ccagaccacc aggccggtaa tcaccgctgc
    11281 cacgccgctc cacaacaaat aaccatttcc gcccagcatc tcggctgcca gcagcaaacc
    11341 gccgagactg agccagaaaa tatgtggatg aacgaccatt aactccatca tggctgagtc
    11401 cgtttgttgg cgctgtcttt caccagctcg gcaatcccgg caatcgaccc catcaggctg
    11461 ctggcctcta atggcatcat cactactttg ctgttactgg aggaaccgat ctgctgtaac
    11521 gcttcggtgt atttctgcgc tacgaagtag ttcaccgcct gaatatcacc ggaggcgata
    11581 gcttcagaca ccattttggt ggcgcgggct tctgcttcgg cggaacgttc acgcgcttca
    11641 gcctgtaaaa acgccgactg acgttcgcct tccgctttca ggatttgcga ctgtttttca
    11701 ccttcggctt tgaggatttc cgcctgacgg atcccttccg cttcaagaat gtaagcgcgt
    11761 ttggtacgtt ccgctttcat ctgcgcgttc attgaagaga taagctctgc cggtgggcgc
    11821 acgtcgcgaa tttcaatacg ggtgacttta atcccccacg ggttggtggc ttcatcgaca
    11881 atacgcagca ggcgtgagtt gatgctgtcg cgctgagaga gcatttcgtc aagttccatt
    11941 gaacccaaca cggtacggat gttagtcatg gtcaggttga tgatcgccag ctccagattg
    12001 ctgacttcat aagccgcgcg cggcgcgtca atcacctgaa taaaacagac tgcgtcgatg
    12061 gtaacgttgg cgttatcttt cgagataact tcctgggaag ggatatcgag cacttgctcc
    12121 atcatattga tcttgcgacc aatgcgatcc ataaacggca ccaccagact gagccccggc
    12181 tgtaacgttt tggtatagcg accaaaacgt tctaccgtcc attgatagcc ctgcggcacg
    12241 attttgacgc ccgcgccgac aatgaccagc gcgacaaaaa tgagaatcgg gataaagata
    12301 agcatcggaa aaacctcctg ttgtaccgtc cataatcagc aaaattgctg cttgattaaa
    12361 caaattatac ctgattactg aaagagagtt cccccttatt cctgcgaagg ataaactgtt
    12421 tttagtaaaa atcagaaaaa gggaacagtg atgcaggaaa atagtccttt gcttcagcta
    12481 caaaacgtag gatatctggc gggtgatgcg aagattctta ataacatcaa tttttcgctg
    12541 cgtgctggcg aatttaagtt aattaccggt ccttccggtt gtggcaaaag tacgctgcta
    12601 aaaatagttg cttcattgat cagcccaacc agcggaacgt tactgtttga aggtgaggat
    12661 gtcagcacac taaagccaga aatctaccgc cagcaagtct cttactgcgc ccagacgccg
    12721 acgctgtttg gcgatacggt atacgataat ctgatctttc cctggcagat ccgtaaccag
    12781 cagcctgacc cggccatttt tctcgatttt ctcgaacgct tcgctttgcc ggacagcatt
    12841 ttgacgaaaa atatcgccga gctatctggt ggtgaaaaac aacgcatctc actgattcgt
    12901 aacctgcaat ttatgccgaa ggttttattg ctggatgaaa tcaccagtgc gctggatgaa
    12961 agtaataaac ataacgttaa tgagatgatc catcgttatg tgcgcgagca aaatattgcc
    13021 gtgctgtggg tgacacacga taaagacgaa attaatcatg cggataaagt gattacactg
    13081 caaccgcatg ccggagaaat gcaggaagca cgctatgaac tcgcataata ttactaacga
    13141 atcattggca ctggcattaa tgctggtggt ggtggcaatc ttaattagcc ataaagaaaa
    13201 actggcgctg gagaaagata ttttctggag cgtcgggcga gcgataattc agctgattat
    13261 tgtcggctat gtgctgaagt atattttcag cgtggatgat gccagcctga cattattgat
    13321 ggtgttattt atctgcttta atgcggcgtg gaacgcgcaa aaacgcagta aatatattgc
    13381 taaagctttt atctcatcgt ttattgccat tacggtcggg gcgggaatta ccctggcggt
    13441 gctgattctc tctgggtcga ttgaatttat cccgatgcag gtgatcccta tcgccgggat
    13501 gattgccggt aacgccatgg tagcggtggg gttgtgttac aacaatttag ggcaacgggt
    13561 cattagcgaa cagcaacaga ttcaggagaa actgagtctt ggtgcgacgc cgaagcaggc
    13621 ttcagcgata ttgattcgcg acagtattcg cgcggcttta attccgacgg tcgattcagc
    13681 aaaaacggtt ggcttagtga gtttaccagg aatgatgtcc gggctgatat ttgccgggat
    13741 tgatccggtg aaggcgatta aatatcagat tatggtgacc tttatgctgc tctcaaccgc
    13801 cagcttgtcg accattattg cctgctattt aacctatcgt aagttttata attcgcgcca
    13861 ccagttggtg gtgacgcaat tgaagaagaa atgatgcgat gccggatgcg cagcatcgca
    13921 tccggcgttg tggttcatgt gccgatcaac ggaatatcaa tacaacaatg catacagctg
    13981 gcggcgatac ttcgacgcca gtgcatcacc cgtacccagc gcagcgagga tctcctggaa
    14041 cgttttacgt gtctgaccgt ctgcggcggt gagatcttta cgcagatgcc cgaacagcaa
    14101 ctccagcgcc tcttcattgc gcccaacctg gtgcagttgc agagccagtt gcgtcgccag
    14161 tgcggcatct tctggattct ccgccacctg ctgttgcaac tgttgaattt ccggcgtatc
    14221 agccgcctgc ttcagcagtt cgatttgcgc caccagcccc tggtagcggg tgtcctgatc
    14281 ctgcaacgga atggttttca gcaccgcttc cgcatcttca gaacggttca gcgcaatcag
    14341 cgtttctgcc agcagcaggc cgatctcccc gttctgattc gacaactgcc aggcgtcttt
    14401 cagcaatggc agggcatcgg tgtaattgcc ttcctgcatc agttgcatcg cctgctgcgc
    14461 tttcagctct tcttcgcgcg gcagcacttt atccagcagg gcgcggatcg cctcttccgg
    14521 ttgcggcccc tggaagccat ctaccggttg cccgttctgg aacagataca cggtcggaat
    14581 cgcgcgcaga ccaaactggg cggcaatcat ctgctccgcg tcacagtcca gcttcgccag
    14641 aataaattgc ccgttgtact gcgccgcgag gctttccagg atcggggtta actgcaaaca
    14701 gtgctggcta cgttcagacc aaaaatagaa cagcaccgga gtggtcatcg actgttcaag
    14761 aacctgttgc aggttagatt cgttaatgtt gacaatattt tctacggaca tggagtcgct
    14821 ctctgttgtc gatattttct ttgacatggg ggcttaagcg cgcgcttcaa ctcacccctg
    14881 caatattttg tccatcacgc gccccggcag caggcgcttg agcaccatta ctgcccaggt
    14941 caccagcgtt accggatagc gcatcttcgg cttctcgcta ataaaagcat ggcgtacttt
    15001 gtccaccacc gcttccggtc ccaacgtaaa gcgggcggcg atgccgggat tttcgactgg
    15061 tttatcactt tgcgtctggt tgacgttgtc ggtgaagcga gtacgaatgg gaccgggttc
    15121 gatcaggctg actttaattc cgctgtggcg cagctccatg cgcagtgcgt ctgaccacgc
    15181 ctccagcgca tatttactgg ccgcgtaagc gccacgaccc ggcgtggaga ttaatcccat
    15241 caccgatgat gtcatcacaa tacgcccttc accgtgcggt aacatcgcgg gtaacaggcg
    15301 catggtgagc tggtgtgcgc cgaaaaagtt ggcggaaaac tgctgttcca tctgcgcacg
    15361 gctgatggtg gaaagggggc catacatgcc gaatccggca ttgttaaaga tcccatacag
    15421 acaattatcg gtcagggcga ttacctcgtc tgctgcgcga tcaacacttt ctggtgaatc
    15481 cagatcgatc aacacgccgg taaatcccat gttgttcata cgctcaacat catccggttt
    15541 tcggcaacct gccagcacat gaaaaccctg gcgttttaat tcgagcgcgc tttccaggcc
    15601 aattccactg gaacatccgg taattaagac cgatttttgc ataactttac ctgtcaggat
    15661 ctccgttgct ttatgagtca tgatttacta aaggctgcaa ctgcttcgcc atccagtcgg
    15721 caataaacgg ctgggcgtcg cggttgggat gaataccgtc atcctgcatc cattgtggct
    15781 tgaggtagac ctcttccata aaaaagggca gcagcggaac atcaaactct ttggcgagtt
    15841 tggggtaaat ggcgctaaag gcttcattat aacggcgacc atagtttgca ggcagacgta
    15901 tttgcattaa caatggttca gcgttggcgg ctttgacatc ctgcaaaatc tggcgcagcg
    15961 tttgctcggt ttgctgtggc tgaaaaccac gcaaaccgtc attgccgccc agttcaacca
    16021 gcacccaacg cggctgatgc tgtttcagca gagccggaag gcgcgccagt ccttgttgcg
    16081 aggtgtcgcc gctgatgctg gcattaacta ccgacgtttt actctgccac ttatcattca
    16141 acaaggcagg ccaggccgcg ctggcagaca ttcgataccc ggcgctcagg ctatcaccca
    16201 gaatcaataa cgtgtccgct gcggcggcac ggaaggttaa caggaccagg aacaggaagg
    16261 gcaaatgcca gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg tcggtcaggg
    16321 ggagcatgaa ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg gcgagaccat
    16381 cgcactggtg ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc tcgccgggct
    16441 tgatgacggc agcagtggcg aagtgagtct ggtgggacaa ccgctacata atatggacga
    16501 agaagcgcgg gcaaagttgc gcgcgaagca cgtcggcttt gtttttcagt catttatgtt
    16561 aattcctacc cttaacgcgc tggaaaacgt cgagcttccg gctctgctgc gcggtgagag
    16621 tagcgcggaa agtcgtaacg gggcgaaagc gttgctcgaa cagttagggc tgggtaaacg
    16681 tctgggtcat cttccggcac agctttccgg cggtgaacag caacgagtgg cgctggcacg
    16741 agcctttaat ggtcgacctg atgtgctgtt tgccgacgaa cccaccggca accttgaccg
    16801 ccagacgggc gataaaattg ccgacctgct gttttccctc aaccgtgaac atggcaccac
    16861 gttgattatg gtgacccacg acctgcaact ggcggcacgc tgcgaccgct gcttacggct
    16921 ggtgaacggg cagttgcagg aggaagcatg attgcacgtt ggttctggcg cgaatggcgt
    16981 tcgccgtcgc tattaattgt ctggctggcg ctaagcctgg cggtggcctg cgtgctggcg
    17041 ctgggcaata tcagcgatcg catggagaag ggcttaagcc aacaaagccg ggagtttatg
    17101 gcgggcgatc gggcgttgcg cagttcgcgc gaagtgccgc aagcatggct ggaggaagcg
    17161 caaaagcgcg gcctgaaagt cggcaagcag ctgactttcg ccaccatgac atttgcaggc
    17221 gacacaccgc agctggcgaa cgtcaaagcg gtggatgata tctacccgat gtatggcgat
    17281 ctgcaaacta atccccctgg cctgaaaccg caggcgggca gcgtattgct ggccccacgc
    17341 ctgatggcgc tgcttaacct gaaaacgggc gacaccattg acgtgggcga tgccaccttg
    17401 cggattgccg gagaagtgat tcaggaaccg gattccggtt ttaacccctt ccagatggct
    17461 ccgcgtctga tgatgaatct ggcggatgtc gataaaaccg gagccgtgca gccggggagt
    17521 cgggtcacct ggcgctataa attcggcggc agtgagaacc agctcgacgg ctatgagaaa
    17581 tggttgttac ctcagcttaa acccgaacaa cgctggtacg gtctggaaca ggacgaaggc
    17641 gcgctggggc gatcgatgga acgctcgcaa cagttcctgc tgctttcggc gcttctgacc
    17701 ttgctgctgg cagtggcagc agtggcggta gcgatgaatc attactgtcg cagtcgctac
    17761 gatctggtgg cgatcctcaa aacgctgggg gcagggcgag cgcaactgcg taagctaatc
    17821 gtcggtcagt ggttgatggt gctgttgctt tcggccgtta ccggtggggc gataggcctg
    17881 ttgttcgaaa acgtgttgat ggtgctgctc aagcccgttc tgcctgctgc actaccgcca
    17941 gccagcctct ggccgtggct gtgggcgctc ggcaccatga cagtcatctc gctgctggtg
    18001 gggctacgac cgtaccgctt gttgctggca acgcagcctt tacgcgtatt acgtaatgat
    18061 gtggtagcga acgtctggcc gctgaagttt tatctgccga ttgtcagtgt ggtggttgtg
    18121 ctgctgctcg ccggattaat gggcggcagt atgctgctct gggcggtgct ggcgggcgcg
    18181 gtagtactgg ctttgctgtg cggtgttcta ggctggatgc tgctgaatgt acttcgccgc
    18241 atgacgctga aatcgctgcc tctgcgcctg gcggttagcc gcctgttacg tcagccgtgg
    18301 tcaacgttaa gccagctttc ggcattttcg ctctccttta tgctgttggc actgctgctg
    18361 gtgttgcgtg gtgatctgct cgaccgctgg cagcagcagc tacctccaga aagcccgaac
    18421 tactttttaa ttaacatcgc cacagaacag gtagcaccgc taaaagcgtt cctcgcggaa
    18481 catcagatag tcccggaatc gttttatccg gtggtgcggg cgcggctgac ggcgattaac
    18541 gataagccga cagaaggtaa tgaagatgag gcgcttaacc gcgaactcaa tcttacctgg
    18601 caaaatacgc ggcccgatca taatccgatt gtcgccggta actggccgcc aaaagccgat
    18661 gaagtgtcga tggaagaggg gctggcgaaa cgcttaaacg ttgccctcgg tgataccgtg
    18721 acttttatgg gcgataccca ggagttccgc gctaaagtga ccagcctgcg caaagtggac
    18781 tgggaaagtc tgcggcctaa tttctatttt attttccctg aaggggcatt agacgggcaa
    18841 ccgcagagct ggcttaccag tttccgctgg gagaatggca acggcatgtt gacacaactc
    18901 aaccgccagt tcccgaccat tagtctgtta gatattggcg cgattttaaa acaggtcggt
    18961 caggtgctgg agcaggtaag tcgggcgctg gaagtgatgg tggtactggt caccgcctgc
    19021 ggtatgttgc tgttgctggc tcaggtgcag gtgggaatgc gtcagcgtca tctggagctg
    19081 gtggtgtggc gcacactcgg tgcggggaaa aagctgctgc gcacgacatt gtggtgtgag
    19141 ttcgccatgc ttgggtttgt ttctggcctg gtagccgcaa ttggtgcgga aacggcgctg
    19201 gcagtgttgc aggcgaaagt gtttgatttt ccgtgggagc cagactggcg attgtggatt
    19261 gttctgccgt gcagcggtgc gctgctgctg tcgcttttcg gcggctggct gggtgcgcga
    19321 ctggttaagg gtaaggcgct gttcaggcag tttgcggggt gaagagagta aataaggggc
    19381 gttctggttg aatggaacgc ctagtttaaa ttaattctac aatatgtttc attgatgatg
    19441 ttattgaatt ggtgatttcc tatctttcta ttgcgatatt aatctgaatc ggtggggttt
    19501 aataatttta aatatagccc tacaaaaata actcagacta ataaaatcat aaatcatatg
    19561 cgttgaatgg atattatcca tatagtgaat ttgttgat
//
LOCUS       DAZFWP020000093        19561 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFWP020000093 DAZFWP020000000
VERSION     DAZFWP020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.093"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..135
                     /locus_tag="ACHK0Z_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             <1..135
                     /locus_tag="ACHK0Z_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HGU4503130.1"
                     /translation="RWHGSAEAHTCREVQDIPGVLAVFAERRKDSFGLYVRLMSVTLN
                     "
     gene            298..666
                     /locus_tag="ACHK0Z_RS15750"
                     /old_locus_tag="pgaptmp_003150"
     CDS             298..666
                     /locus_tag="ACHK0Z_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4503131.1"
                     /translation="MSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGVCLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            756..1082
                     /locus_tag="ACHK0Z_RS15755"
                     /old_locus_tag="pgaptmp_003151"
     CDS             756..1082
                     /locus_tag="ACHK0Z_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4503132.1"
                     /translation="MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDN"
     gene            1130..1618
                     /locus_tag="ACHK0Z_RS15760"
                     /old_locus_tag="pgaptmp_003152"
     CDS             1130..1618
                     /locus_tag="ACHK0Z_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4503133.1"
                     /translation="MKLALTPEADSVNVQALNMGRIVVDVDGIELAELINVVCDNGHS
                     LRVVDESDRASTDCTPSFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRQGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            1630..1827
                     /locus_tag="ACHK0Z_RS15765"
                     /old_locus_tag="pgaptmp_003153"
     CDS             1630..1827
                     /locus_tag="ACHK0Z_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4503134.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLQQNR"
     gene            1912..2754
                     /locus_tag="ACHK0Z_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             1912..2754
                     /locus_tag="ACHK0Z_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4503135.1"
                     /translation="MSDITISRPELVNRHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISAMTESIGGKTAPAWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYQSLEYDNFPEISEANILSTFEQLHQNKDEVFERGMINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQCKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAM"
     gene            complement(3097..3633)
                     /gene="yghD"
                     /locus_tag="ACHK0Z_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             complement(3097..3633)
                     /gene="yghD"
                     /locus_tag="ACHK0Z_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HGU4503136.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIADRGENIQVWIDPVVFNDLLKWLNALDEKYALRVTQIDAS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(3635..4813)
                     /gene="gspL"
                     /locus_tag="ACHK0Z_RS15780"
                     /old_locus_tag="pgaptmp_003156"
     CDS             complement(3635..4813)
                     /gene="gspL"
                     /locus_tag="ACHK0Z_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGU4503137.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMACDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGKP
                     EGNLLTGPWQPRVSYRKQWARWRVMILQILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(4810..5787)
                     /gene="gspK"
                     /locus_tag="ACHK0Z_RS15785"
                     /old_locus_tag="pgaptmp_003157"
     CDS             complement(4810..5787)
                     /gene="gspK"
                     /locus_tag="ACHK0Z_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGU4503138.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNETELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5790..6389)
                     /gene="gspJ"
                     /locus_tag="ACHK0Z_RS15790"
                     /old_locus_tag="pgaptmp_003158"
     CDS             complement(5790..6389)
                     /gene="gspJ"
                     /locus_tag="ACHK0Z_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGU4503139.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6386..6757)
                     /gene="gspI"
                     /locus_tag="ACHK0Z_RS15795"
                     /old_locus_tag="pgaptmp_003159"
     CDS             complement(6386..6757)
                     /gene="gspI"
                     /locus_tag="ACHK0Z_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGU4503140.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(6754..7317)
                     /gene="gspH"
                     /locus_tag="ACHK0Z_RS15800"
                     /old_locus_tag="pgaptmp_003160"
     CDS             complement(6754..7317)
                     /gene="gspH"
                     /locus_tag="ACHK0Z_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGU4503141.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(7321..7776)
                     /gene="gspG"
                     /locus_tag="ACHK0Z_RS15805"
                     /old_locus_tag="pgaptmp_003161"
     CDS             complement(7321..7776)
                     /gene="gspG"
                     /locus_tag="ACHK0Z_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGU4503142.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7793..9016)
                     /gene="gspF"
                     /locus_tag="ACHK0Z_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             complement(7793..9016)
                     /gene="gspF"
                     /locus_tag="ACHK0Z_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGU4503143.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPAMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFSP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9016..10509)
                     /gene="gspE"
                     /locus_tag="ACHK0Z_RS15815"
                     /old_locus_tag="pgaptmp_003163"
     CDS             complement(9016..10509)
                     /gene="gspE"
                     /locus_tag="ACHK0Z_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGU4503144.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(10509..12569)
                     /gene="gspD"
                     /locus_tag="ACHK0Z_RS15820"
                     /old_locus_tag="pgaptmp_003164"
     CDS             complement(10509..12569)
                     /gene="gspD"
                     /locus_tag="ACHK0Z_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HGU4503145.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(12599..13558)
                     /gene="gspC"
                     /locus_tag="ACHK0Z_RS15825"
                     /old_locus_tag="pgaptmp_003165"
     CDS             complement(12599..13558)
                     /gene="gspC"
                     /locus_tag="ACHK0Z_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGU4503146.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(13576..13986)
                     /gene="gspS2"
                     /locus_tag="ACHK0Z_RS15830"
                     /old_locus_tag="pgaptmp_003166"
     CDS             complement(13576..13986)
                     /gene="gspS2"
                     /locus_tag="ACHK0Z_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HGU4503147.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(14052..14861)
                     /gene="pppA"
                     /locus_tag="ACHK0Z_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             complement(14052..14861)
                     /gene="pppA"
                     /locus_tag="ACHK0Z_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HGU4503148.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAIITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(15009..>19561)
                     /gene="sslE"
                     /locus_tag="ACHK0Z_RS15840"
                     /old_locus_tag="pgaptmp_003168"
     CDS             complement(15009..>19561)
                     /gene="sslE"
                     /locus_tag="ACHK0Z_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HGU4503149.1"
                     /translation="FKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEV
                     KPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDI
                     TCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELA
                     GSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVN
                     EEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGR
                     LVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIAL
                     TELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGAT
                     LDEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGV
                     INKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYW
                     LAFGEKRAWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVIGNPH
                     YNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKSSMTV
                     GTNLDTVYFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNGFEYV
                     TQWGSDPYSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFV
                     RLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTK
                     EVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQRILD
                     AFPGLEVCKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLDLGADTAKAMLQAADL
                     GTNIQRLYQHELYFRTNGLQGERLNSVDLERLYQNMSVWLWNETKYRYEEGKEDELGF
                     KTFTEFLNCYTNNAYVGTQCSAELKKSLIDNKMIYGEESSKAGMMNPSYPLNYMEKPL
                     TRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAP
                     AQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVP
                     YGGLIYIKGDSKDNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTAP
                     KKNLNASNYTGGLKQFANDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFAND
                     VQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVA
                     NNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAE
                     KNFDIKKWYPEGSLPAFYSEREGMKGWNLFQLMHRKARGDEVGKTKFGERNYCAESNG
                     NAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVGQSAYNTLASLK
                     LPKPEQGPETINQVTEHKMSVE"
BASE COUNT         4419 a         5522 c         4931 g         4689 t
ORIGIN      
        1 cgctggcacg gcagcgctga agcacatacc tgtcgtgaag tgcaggatat tcccggcgtg
       61 ctggctgtgt ttgccgaacg ccgtaaggac agttttggcc tgtatgtccg gctgatgagc
      121 gtcaccctga actgatgttc cgtcacccgg aaaacgagta cgactggctg agctcagacg
      181 ccgctgtgga tgaggtcctg ctcatcaacg aatacacctt taccgaagcc ggtctgcgcg
      241 ccggctgacc cgtcatcgtt aaactatcat tcaccgattt tctgtgagga ttttatcgtg
      301 tcagacacac tctccgggac aacgcatccc gatgacaatc acgaccatcc ctggtgggga
      361 ctaccctgca cagtgacgcc ctgtttcggc gtctgtctgg tgcaggaggg taaccggttg
      421 cattaccttg ccgaccgcgc cggtatcaga ggcctgttca gcgatgcaga tgcgtaccac
      481 ctggaccagg cctttccgct gctgatgaaa caactggagc tcatgctcac cagtggtgaa
      541 ctcagccccc gctatcagca taccgtcacg ctgtatgcga aaggactgac ctgcgaagcc
      601 gataccctgg gctcctgtgg ttacgtttat ctggctgttt atccgacgcc cgaaatgaaa
      661 aattaactct ccagaatagc cttctgctac ggcctggtgt tttcaccacg ccactttccc
      721 atttctcatc tctgcatatc aggaaaacct tcagtatgaa aacattaccc gatacacacg
      781 tacgggaggc atcgcgctgc ccgtctcccg tcatcatctg gcagacactg ctcacccgac
      841 tgctggacca gcactacggc ctcacgctga atgacacacc gttcgctgat gaacgtgtga
      901 ttgagcagca tattgaggca ggcatttcac tgtgtgacgc ggtgaacttt ctcgttgaaa
      961 aatacgcgct ggtacgtacc gaccagtcgg gattcagcgc ctgtacccac tctcagttaa
     1021 taaacagcat cgatatcctc cgggctcgcc gggcaacagg cctgatgact cgcgacaatt
     1081 aaagaacggt aaataacatt accctgggta aacatccagg ggcgaaacaa tgaaactggc
     1141 cctgacaccg gaagccgaca gcgttaacgt acaggcactg aacatggggc gcattgtcgt
     1201 tgacgtcgat ggtattgagc ttgctgaact gattaacgtg gtctgcgata acggtcactc
     1261 ccttcgtgtt gttgatgaat ctgaccgagc ctcaacagac tgcacgccat catttgctgc
     1321 cctgactggc atacgctgca gtaccgcgca tatcacggca aaagacaacg cctggctgta
     1381 ctcgctgtcg catcagacga atgacaccgg cgaatcagaa tggattcatt ttacaggcag
     1441 cggatatctg ttacgtaccg atgcgtggtc atacccggtt ctgcggctta agcgccaggg
     1501 cctgtcaaaa acgttccgcc gtctggttgt cacactcatc cgacgttatg gcgtcagtct
     1561 cattcatctg gatgccagcg ctgaatgcct gccgggttta cccactttcg actggtaacc
     1621 aggaacaaca tgaaatcatt aaccacggaa accgcactgg atattctgat tgcgtggctg
     1681 caggacaata tcgactgcga atcgggaatt atctttgaca acgatgagga taaaacagat
     1741 tcggcagcac tgctgccctg cattgaacag gcgagggagg atatccatac cctgcgccaa
     1801 ctgcagcttc tgcaacagaa ccggtgagtc ttactcacca gacttcattc cactgacgcc
     1861 agcctgaata cggctggcgt tttcgtttat aaaccaaaag gaaaaacgat gatgtcagat
     1921 atcacaatct cccgcccgga actggtcaac agacatacgg acgttatctg ctcaaccagt
     1981 atccgccaca ttttggctgt acgaaagagt gcgctgctgc aaatcgacac acttatccgg
     2041 caactggctg aaatctcagc aatgacagaa agtattggcg gtaaaaccgc cccggcctgg
     2101 gccatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga aaccgcaatg
     2161 aaagccatca cccggaatct tgaccgcgaa atctggcgtg acctgatgca acgttcaggg
     2221 atgctttcct taatggatgc acaggcccgt gatacatggt accagtcact ggagtacgat
     2281 aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccagaac
     2341 aaagatgaag tgtttgagcg ggggatgatc aacgtcttca ggggactgag ctggaattac
     2401 aaaaccaatt gcccctgcaa gtttggcagt aaaattatcg tcaacaacct ggtaaggtgg
     2461 gacagatggg ggtttcatct tatcaccggg caacaggccg atcgacttgc cgatctggaa
     2521 agaatgctgc acctgttcag cggcaaaccg atccccgaca accgggaaaa catcaccatt
     2581 catctggatg accacatcca gtctgttcag tgtaaagagg actatgaaga tgagatgttc
     2641 agcatcagat actttaagaa gggctccgca cacatcacgt tcaggaaacc agaactggtt
     2701 gacaggctta atgatattat tgccaagcac tatcccggag cgttagcgat gtaaaaagga
     2761 ataaaactca ggaataaaac ccacaaaaat acacgtgttc tgatgacaaa aatcttgatc
     2821 tacttgttct cataaaaacc accagaaggg accgagggta caagagaggg tacatttttc
     2881 atcaaaccaa ataaacgaga ttaatatcaa catgataaca accatattcg attccgagtc
     2941 cggcaccact aattcttaag aacccgccca caaggcgggt ttttgctttt ggatctgaca
     3001 ataaccttca cgaaaaaaat tagcttataa agtctggggg aattactctc gccacgttaa
     3061 cgagagtaat tttattgata ttaatctcct gatactttac ccccgtccaa actccagccg
     3121 ctgcacattc accatcccag gcttctcagc agcactggca tcaatttgtg tcacccgcag
     3181 cgcatacttt tcatccagcg cgtttaacca tttcagcagg tcattaaaca ccaccggatc
     3241 tatccagacc tgaatattct ccccacgatc ggctatccgc ttgatgacta ccgagtgcgc
     3301 ggaagcactg tcactgatga cccgcgatac ctgcgcaggc gttgttgtgg cagattttcg
     3361 cgctgcaata atatccggcg cggcgttctt cagtcgggtg ttcatcgcca ccagttgctg
     3421 caacattgtc tcctgttgct caatccgttc gctcaaaggc tgccagatga ggacgtaata
     3481 tccgacgcta aacaggaaca ccaccgctgc cagtaacatg cccttttcac gcggagaacg
     3541 ccccgccagg tgttgtgcca gccagtgttc gccacggctt aactggcgtt cacgccattg
     3601 ctgaaaatag tgaataaatt tatcgcgtaa catgttattt cctccgcaac gttacgccgc
     3661 cggaaaccgc atcaccctct ttctgtaacg cgtcctgttg cacaacataa tcggttgcca
     3721 gtgcactacg cagtttgtcg aaactggcaa agttcgcggc ccgtagctgg aggtgaagta
     3781 tctggcgttt ttgatcaaag gtaaaaccac gcatttcgat gtcggaaagt gacgctgatt
     3841 tcagggtgct ggctatcgct gacaactcgg cgagcagccg ggtatcgtcg gcctgtgggc
     3901 gatatttttt cagcgccatc gtcacctgag agcgtaaatt cacaatccgc ttctgctccg
     3961 ggaacaacgt taagaactgt tcctccgcct gggtgcggct ttgcgccacc tgttcgctga
     4021 cgctccataa cgtcacgccc cgctccactg ccagcgcaac cagaatcagc aatatctgca
     4081 gaatcatcac ccgccagcgc gcccactgtt ttcggtagct gacgcgaggc tgccacggcc
     4141 ctgtcagcag gttcccttcc ggtttgccat aagtggtaac ggcgggcaga gctgtaacgg
     4201 tcaggcgttc agcgtctgcg ccagcccatg ctgatagctt ttccggtgca atgccgacta
     4261 cggttagcga aagcggtaaa tcctgctcat tgagctgggc gcggaacatg accggagcca
     4321 gcgccagccc ggcgctccat ccccggcatt catcgatgcg gcagataacc cgttgcgcat
     4381 cgcaagccat aaacccacaa ggaatggaca tccagtccgg cgcgacggta gcgcgggtga
     4441 tgccgttttc ctgcaaccac tgcgcaatgt tgcgcatatg ctgttggtga atcactgcta
     4501 cggttgccag ttgctggtcg atattcaacg gggcgaaatg cagttcatcg atatcctggt
     4561 tcagctcttc ttccagtaag gcgggcagaa tggtcggtat ctgcttacgg gcgacatcag
     4621 gcagttcaac ctgccagacg ctgatccatt cgccgggaat gtagagtcga atcgcatcgg
     4681 tttgcagcca ttgctggagc tgttcgttag caacgtcaga ccagataccg tgctccacat
     4741 cgggtgtacg gcgctgccaa tggatgggat cagcggcgca aagcgggaaa aaaatctcaa
     4801 ggatggaact cactcacttt ctcctgtctg atgccagaga accgaaaagt gttgtgggcc
     4861 catgcggaca attaacgagt tcatcgtcag ttcagtctca ttcacggtga tatctgaacg
     4921 cagccagaag taattgctgt ccacgctcag gacggttttt agctgttttt tagtacgctc
     4981 atcgacgtca gcaagtagcg gctgtgcaag aaactgatcg acatcttccc agcccttcgc
     5041 cggacgttgt tgtaataacg cccgcgcctg aacggggctt aaccacgggt caaacagcgc
     5101 ctcaagaatc acactttgcg tgacgtctaa agtattgatg ttgatttgct ggcggatcat
     5161 cggcagcgca cagaccagcg gtttcagttt ttgataaagc ccggcgtcca ttccctgtac
     5221 cacgcgcatc tcactgatat cagccagcgg ttgattcgcg gcgtagaaag gcaccgaacg
     5281 ggcgagatac tcgctgtctt cacggcccag acgtgtctgc acgctgcggt cctcgtcaat
     5341 aaactcccac aggctttcgg ctatcagttc ggcccgataa gcaggcacat ccaggcgcga
     5401 gatcagggca atcaactgtt gtaccgcaag cggacgcgac gccgttgtcg gctgagcaag
     5461 ggcattcagg ttaaagcaag cctgtgcgtc acgcagggtg accgcaattt gcccctgcgg
     5521 cagagggaag aaacgagggc cggatgccca cggctgtgcc agatgcacgc gcttttcatt
     5581 tttcaggctc agactgagcg cgctcagcgc caggctttcc gcactggcgc tgtaccacag
     5641 cgcctgctgg tactcctgct ggctgcgcgt tcgcccaagt tgttgctgca tccgcccgga
     5701 aagcgtgatg gttaccagca tcataaccgc cagcaatacc agcaccacga ccagtgccat
     5761 tccgcgtttt ggtggtgagg tgatcatgat tattgcggcc cgcgtaacaa ccagatgcgt
     5821 tcaatctcgc cccattgcgg cgaatgcagg gtcatgcgta ccgccacagg gatcgcctgt
     5881 actgacgacc agctctcctg ccagcgcgtg ccgtcgtaga actgcaaacg gagcgaatcg
     5941 gccggaatca acttttgcat tgtcggcttc acgctgcctg cggcatcggt cagcggccag
     6001 gctaaccgtt cgagataacc gtcatgaatg cggtaaccga cggtgagcag attactgcgc
     6061 ggcagacgca tcaacgggtt aaccacaccg ccgcgcacaa aacgcattcc ttcactctca
     6121 gacgccagca cgccagcgcc agccagtaac gctggttcac gctggccctg atcgcctctt
     6181 accggacgcg gcatcatttg ggtcagatcg tgggtcagaa aactcatcgt ttgctgcatg
     6241 aggtttagtt tttgatcgtg tccggcgacg gcgctattca cgcgcgtgac gccgttagtc
     6301 acctgctgcg ccatcagtgc cagcgaggca aaaatggcaa ttgccaccag catttccagt
     6361 aacgtgaaac cagcgcgagt ccttctcact gttggcctcc cacggcgcta aaccaggcgc
     6421 gtcgtgactg aatcaccgac gaaaagtctt cgtgacggct gacttcaata tccaccgcct
     6481 ggagcagcgc gttgcctgta ttcaatggga ttgttcgcca gtaccagcgg cttcctgcca
     6541 tatcgctctc accctgatgc ctgaccgccc gctgctcacg ggtcatcagg tagagcagcg
     6601 cagtctggtt ttcagctacc cagcccgcta ccgttttttc ctcaagaacg tgctgattac
     6661 tcagcgcacc gctggcaatc tgtaacaccg ccgtggcagc cagcgcaaaa atcgccagcg
     6721 cgagcatcac ttccagcaag gtaaatccgc gcttcatggc atcctctcat cacattgatt
     6781 gagggataac gcgccatcgt gtgccagctt taccgcccaa catgcgtttt gcgccgccga
     6841 gtagaagcgc agcgtaaacg gcgtggcggg ttcaaaaggc gaaaaacgga tctgcggcgt
     6901 cttctttttc gcctcttttt gcagctcaag ttcaatatcg tgcagcgtca gtcgacgacg
     6961 ttgcagctcc agcgcatatt ccttctgcca gatatcactt ccgggttgta acagcatctg
     7021 cacctgtttc ggcaccgtaa cctgtgccga taaccgggtc gaggaaacgg gcgaccactg
     7081 cccctgacga cgctgcataa actggtagcc aggcgggtcg attagcacac cgagtgtttt
     7141 cccttcgatc actgccctgt ccttaaactg cgcaaagcga gtcagaaaat cctgcgccgc
     7201 ttttttcgcg ggcgattcag aatcggtcgc aaacgtctgt actacgcccg cactggcaag
     7261 gccgataagg aaaatcacca gcatgatttc cagaagtgtg aatccgcgtt caggcactgc
     7321 ttactgaaac tcctgcaaat tccagttacc gatatctgcg ccagcgcctt ccccgttttc
     7381 ctgaccatcc gcccccaggg tataaacatc aaacagccct ttttcacccg gactgagata
     7441 ctgataatca ttgccccacg gatcctttgg cagtcgttta atgtatccac cagtacggta
     7501 gttgcgggag tccgccatat tggctggctg ctggatcagc gcctcaagcc cctgttctgt
     7561 ggtcggataa cgcccgttat ccagtcggta catatccagt gcgttttcca gcgccacgat
     7621 atcgctgatg gctttttgcc gatcggcttt ctctttgttg cccaacaggt taggcaccac
     7681 cagacttgcc aggacgccaa gaataacaat caccaccatc acttccagca gggtaaaacc
     7741 tgcccgtggt ttttgtgtgc gggataacga attcatgtga taactccgta aattacattc
     7801 caaccatatt gttcagttgc agcatcggct cgaggatggc gatgacaata aacagcacca
     7861 cgcccgccat catcaccacc agcgcaggct caaacagccc caacgccagc cccacctggg
     7921 tatcaaattc ccgttcctgg ttgactgcgg cctgctcaag catggtttcc agttcgccgc
     7981 tctgttcgcc ggaggcgatc atgtacagca tcattggcga gaacagccgc aactccgcca
     8041 gcgcggcgcg cagactgctt ccttcgcgga cgcgatctgc cgccagcagt agttgttgtt
     8101 cgacatagcg atttgccgac acggcagcag cggtctgaat gccttccagt agcggaacac
     8161 tgctggcggt gaggatgctt aaggtgcggg aaaaacgcgc cgtattcagc ccgcgcgcaa
     8221 cacgccccgt cacaggcagg cgcagcacgg ttttgtccca gcgcaggcgc atagcaggat
     8281 ttttaagtag ccgttgcccc agcaccagaa gcgccagcaa tcccgccagc cagtaaacgc
     8341 cgctggcctg taacgcatca ctcatggcaa taagcgtgcg ggtggaggag ggtagcgcgt
     8401 gtccgagatg atcaaactgt tcgatgattt tcggcaccac tgccgtcagc aaaatagtga
     8461 ctacgcccgt tgccaccacc agcagaacca gcggatagag catggcctgc aatagacgtg
     8521 atttcaggcg ctgtcgctgt tcggtgtaat ctgccaggcg attgagcacc acgtcgagat
     8581 gcccggattt ttctccggca gcaaccatcg aacaaaacag agaatcaaag acgcggggat
     8641 gttcgcgcag gctgtccgac agggtgtaac cttcctgaat ccggctgcgc agcgccattc
     8701 cgaggctttt tacatgcagt ttttcacttt gctcactgac cgcctgtaag caggtttcca
     8761 gcggcattgc tgcctgtacc agcgttgcca gttggcgcgt gaacagcgca agatctgccg
     8821 ccgccacgcg acgatgtgcg tgccgccgac gctgcaacat cccccctgac gaagtattca
     8881 tccgggcttc aatatgcacg gggataagct ctttaccgcg caacaactgg cgggcatgac
     8941 gcgcggaatc cgcctcaatc atacctttgg ttttgcgacc attacgctcc agcgcctgat
     9001 agtaaaacag tgccattacg cctccatggt tacccgcaga acttcatcga gagaggtttc
     9061 tccggcgagc actttctcaa tgccgttgct gcggataccc gcagagtgtt gtcggacata
     9121 acgttccagc tccagctccc cggcctgacg gtggatcaaa tcacgcaatg tggcatccac
     9181 cacgatcagc tcatggatgg cagtccgtcc gcgaaaacct ttgtgattac aggcgggaca
     9241 gccctgtgga tggtacagag tgacggtacg ggcgtcggta attcccagca ggcgtttttc
     9301 ttcgtcagtg gctggtgcgg gctggcggca gtcgggacac agcgtgcgaa ccagtcgctg
     9361 cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca tatcctgcaa
     9421 acgtgtgatc gcccccaccg ctgtgttggt atgcagcgtg gaaaggacca ggtgtccggt
     9481 aagtgaagcc tggacggcga tttctgcggt ttcggtatcg cggatttcac cgaccatcac
     9541 cacatccggg tcctgacgca aaatcgcgcg cagcccacgg gcaaaggtca tgccgacgcg
     9601 ggtgttaacc tgcgtctgac cgatcccttc aatcatgtat tcgatgggat cttcaaccgt
     9661 gagaatgttg cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg tggtgctttt
     9721 gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta acagctggcg
     9781 caactgcgcg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc gggcctggtt
     9841 tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg tggagacacg
     9901 cacgtcaatc gcccggccgc ccagcaacag cgcaatacgt ccatcctgcg gcacgcgctt
     9961 ttcggcaatg tccagccgcg ccatcacctt gatacgcgac accagcagcg aggccagttt
    10021 gcgccccgga cgcagcattt catgtaatgt gccgtcaaca cgaaaacgga tcaccagact
    10081 cttttcaaac gtctcaatgt ggatatccga agcgccttct ttgattgcct ctgccagcat
    10141 ggcgttgatc agtttgatga tcggcgcatc gtcgtcgctt tccagcagat cttccgtttc
    10201 cggcagttct tccgcgaggg taaaaaagtc ctcggcagaa ccgagatctt ccatcagctg
    10261 gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa attcggcctc
    10321 gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct gcaaaacaga
    10381 gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg ccaccaggct
    10441 aaaccgacgg ctgaaactgt agggcagacg cacggtgttg gcggtggttt cctgtgctac
    10501 aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg cggtaaggcc
    10561 tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa accttgctca
    10621 tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac gccgtctgcc
    10681 gccataccgt cacgcagaat ggtcggacgg ataaacacca tcaggttacg tttttctttt
    10741 ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag cagcggcact
    10801 ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat cagctcacca
    10861 tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc gaggctggtc
    10921 tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt accttcgtta
    10981 atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt attgaaagga
    11041 ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtcctg gcccaccatg
    11101 aagaaggctt cctggttgtc cagcgtggtg atgctcggcg tggagagcac gttcgagctg
    11161 gagtcgtttt taaccgcctg caccagcgcc atccagtcgc ctttcaccac accaaccgcc
    11221 gtaccgctaa agccggaaag aagctgggcg agtgtggaga gatcgccgtt ggtatccgga
    11281 ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg tttcgcctga
    11341 gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa ctgcattaat
    11401 ccggcatctt tcgacgccca ctgcacgccg aagttgatat tgctaccttc ggcaacttcc
    11461 acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc aattacgctt
    11521 tgcagcgact gcatgatgtc ctgcggcgcg gttacaatca gggcattact gtgtttgctg
    11581 gcggcgatgg atacaacctc acgcccgcta ccgaccgtgc cttccgcctc ttctttagcc
    11641 gccgtgagcg tgccgctgac ctgtttcagc acatcgacca gatcttcggc tttgctgtat
    11701 ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag ccgacggatc
    11761 aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact gttggtgcgt
    11821 tcgtcggcga caatttgaga tttcagcgtc gcaggctggt tctcgccgct gtttttcgtc
    11881 aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg gatcacctct
    11941 tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ccgtcaggcg ctccacaacg
    12001 gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac gttgcctgag
    12061 cctgcgctgt cgatcatctg gcgcagaatc ggtgccagct cgcgtaccga cacattacgt
    12121 accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc ttcaccgacc
    12181 agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac gtcgttttcc
    12241 atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg gtaatactgg
    12301 cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc cggccccata
    12361 atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag gtcggtatct
    12421 ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca taatgccgct
    12481 gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt cttacgccag
    12541 accgacaacg taatatcacg ccaaaacacg atgaatgatt ctcctggatt aaatgcggtt
    12601 agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta agttgaatgg
    12661 aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca gtgaaatcct
    12721 gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg aacagagaac
    12781 gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca ggcgtaagct
    12841 ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg gcagtcggga
    12901 tctccactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg tcactgacgg
    12961 cttttttgtt ggtcacggct acggtggaat gcccctcttc tgccaggctc aggcgctcta
    13021 tttttccctg atagcgcagc atcacatggt cgcgattgat ttcctcaatc accgcattgt
    13081 gcgagccaag cgtttcaccc tgcaaataga cctgctgttt accgccttct tcaataaccg
    13141 cgccgggtct ggcaccaaag gcaatcccgc gcagcaccac attaagacgc gtttctgcca
    13201 caggcacagg ttcgggttgt tttaccggcg tggcgacggg ctgatatttg ccaaaccagt
    13261 tttgctggct gattaattgc acgtcatttt tatcgaacgt ttttgcatcc gcacggggcg
    13321 gtttattcgc cgatggggaa acagccgtat attccgcaga aaaggagaaa tagcgccaga
    13381 gtgaatgcgc cacttttgca gaaataataa gcagcatcag ccagaacatc ccgcgtacaa
    13441 tttttcgcag atgctctttg tctgtattaa aagattgtct ctggttaaga gtgtgacgaa
    13501 tttttgtcag ccattgaatg agataaattc gtgcgtcacg aaaaacaacc cgcgccaatt
    13561 tatatctacc cgacgttatg ctttgactat tccacaggtg gtacgatcca gtttccgctg
    13621 atactggtta cctgtacgtg tatcattaat cgttatgctg taattaattc cctcggtaat
    13681 cattaatttc accgttgggt cagcgcacat ttgtcgttga tagctggtta aaaaggcgtc
    13741 aggcgtctgc gtggtctgag tccccgcttc gctgataatg gtcattttta ccgtcgtgcc
    13801 gctactttgt gccagcacta aggtatatcc cgcagattta atcggcaggt tttggctgat
    13861 attttgcgcc tgttttttcg ccagtaaact ggcattttca ttatggctgg cacagccact
    13921 taataatcct gtaacgctca acaatagcga tattaatacc ctccctggca tttgttttat
    13981 cgacatagta aataatctct aaggttatta ataagagtta aaatgtcact ttgataatga
    14041 cgtggttatc attaaaacaa tgcctgtaga taaagtgttg ctataccgcc cagacttaaa
    14101 cacggtccaa aaggcagtgt ggttgatcct ctttttgtaa taatggcata tatcaggccg
    14161 cagcatgatg cgattaaagc aacattgggc agtgataacg gccccaccca gccacctaac
    14221 gcagcgaaca ataatacatc gcccatgcct aatgcttctt tacgcagaac tattccggct
    14281 atccagcgca gggagtaaaa agtgataaac cccaccagga cgccggtaac tgcgtcttgt
    14341 agcgttaacg gactctgttg cgcccatgcc gcaatcagtc ctgtccacaa tacgccctga
    14401 gtaaaaacat cgggcagcca ttggtgatcg aggtcgatga tgctcgcggc aatcagccag
    14461 gcggataata tcatcaccgc cagcgcccat ccactttccg gccagaccag actcgccagc
    14521 aaaaaagcga gtgccgtcaa taactccacc agcggataac gcttgctgat tttcgcctga
    14581 cagtcgcggc agcgcccttt gagcatcaac caggagagca gcggaatatt gtcacgaacg
    14641 cggatggtct gctgacaatg tgggcagtgc gaacgcggta gcgcaaggct tatttttgac
    14701 tgcgcactcg acatttcacc gtgaaactcc gccatttgtt ggcgcagcat gatggggtaa
    14761 cgccaaatca ccacattcaa aaaactgccg atgatcaatc ctccgacggt tgccagtatg
    14821 ggcatcgccg cggggtattg ctgaaaaaca tcaaaaagca tggttaaagg ttgtttgttg
    14881 taacttgctg gatgcgggcc tgcaagtgta tgccatacgg ctttcctcca ggcccgataa
    14941 gctggcgcaa cccggtacgc cttatttcat gccggatgcg gcgtgaacgc cttatccggc
    15001 atacaggatt actcgacaga catcttatgc tcggtaacct gattaatggt ttccggtccc
    15061 tgttccggtt tcggcagctt gagcgacgcc agcgtgttgt atgccgactg gcccacaccg
    15121 ccctcgaagc tcatctcgct cgcccccggc agctggtaag cattcgcacc cggattccat
    15181 ttcttaaaga actccgaaag atccgtctgg gcgacccagg aggcacacag catcagcgtg
    15241 tccgcagcgt taccgtttga ctcagcacag taatttcttt cgccaaactt ggttttacca
    15301 acctcatcgc cgcgtgcttt acgatgcatc aactggaaca gattccagcc tttcatccct
    15361 tcacgctcgc tgtagaacgc tggcagagag ccttctggat accatttctt gatatcaaag
    15421 tttttctctg cccattcctt cagttgtgcg tacatcagca gacggtcacc cgcaccgccg
    15481 cgcgcccatg cctgaccgtt gctctcctcc agatattccg gtgcgacggt aatatcgtca
    15541 gcgacacggt tcatcttgcc gagataacga tcctgcatgt acagcgccag cacgttgttc
    15601 gccacttcgg tcgcgcctgg aacagtcagc ggcgtttctg cggcgttgtg ccctacttcg
    15661 tgccagatca gccagtcgtt cagcggcgtc gtcggcagcg tggtgctgtt cgtcgagaag
    15721 ctgctgttca ttaccggata acccgaatgc gcatcaccga tggagatctg cacatcgttg
    15781 gcgaaacgat gcttgtggcc cgtcaagttt ttataggtaa acatccggtg cttaccgtct
    15841 tcatcattac gaccgtagaa atcattcatc gagctggcaa aggtatccag atcgttagcg
    15901 aattgcttca gtccgccagt gtaattgctg gcattcaggt tcttcttcgg tgcggtataa
    15961 acgaaagcgt ctgattccag ctcacccagc ggagccggtg agttcagatc gtttttccat
    16021 gcgccgtctt tatagaacgg tgcttttacc acaccagtaa aggtgaagct ggcagattca
    16081 ttgtctttgc tgtcgccttt gatataaatc agaccaccgt aaggcacctt gaatttcacc
    16141 gtaccactag cgtccagaga gtacgttttg gttactcttg gcggacggtt cagcgcgact
    16201 tcatgcttct ctcttccggt caggtcgtca gccagcgcca cggtgacggt cacaggaacg
    16261 ttcgcattgg acttaatggt gacctctttc tgagccggtg cccacaggcc agttgactgc
    16321 atgttacctg caaaccattt ggtcggattc gagtacaggc tgatggtttc agtaacgttc
    16381 tgtccctctt ccgatactgc tcccggatac ttctcgacat caactttgat gttcagatcc
    16441 caccaggaac ggcccagcat caggcgtgtc agcggttttt ccatatagtt gagcgggtag
    16501 ctcgggttca tcatgcccgc tttgctgctt tcttcaccgt aaatcatctt gttatcgatc
    16561 agcgattttt tcagctcagc ggaacactgc gtgccaacgt atgcattgtt ggtgtagcag
    16621 ttcagaaact cagtgaacgt tttaaagccc agctcgtctt ctttaccctc ttcataacga
    16681 tatttcgtct cgttccacag ccagacggac atgttttggt ataaacgttc cagatcgacg
    16741 ctgttgagac gctcaccctg gaggccattg gtacggaaat aaagctcatg ctgatacagg
    16801 cgctgaatat tggtgcctaa atccgcagcc tgcagcatcg ctttcgcagt gtcagctcca
    16861 agatccagct gcgtatactg cggaacatac atgccaccgg ttaccggcac acccgtgcct
    16921 gggcggtatt ccagacagtt gacctcatag tgatagtcag aatccttaca cacttccagc
    16981 cctgggaacg cgtccagaat tctctgtttt gccgcagcca gtgactcagg cgttttgtgg
    17041 tcggcttcat caataaaggc gaattgggtt acctgtttac cctcaacttc ttcctgccag
    17101 ctggcaactt ccagcttcgg tttgtcatca ggtttgtttt cttgctgata tttccagata
    17161 acttctttcg tggtgtcatc aatggtatac ggtggtttac catcgacagc cggataacgc
    17221 tcatagaccc aaattggcgt tgaacgtcgt tgacgaacac ggtcagggta gccttgcgga
    17281 tcgttattta ctaccgactt gttaagcgcc atcgacaaac ctgcggcatc aagcagacgt
    17341 acaaagccag atgcgctctc ttccttaaga ttgctcatca cgttttccat gatcagcacc
    17401 gatccacctt tgttcatata ggcgatcaaa tcggtcacat cctgctgggt cagcttcggt
    17461 ttgctggtat ctgcgcgcag aggaatggag taaggatcgc taccccattg tgtgacgtat
    17521 tcaaagccat tgaggatcaa cagaggaact tcatctggat tcagattgcc atagctggtc
    17581 attggtttga ccgtgatgcc agtgaaatcc ttgtggaacg caaacggtgc gctatttccc
    17641 agcacctgac catgtttttt gaaatatacc gtgtccaggt tagtacccac ggtcatactg
    17701 gattttgcat ccggcaacca gcgatcattt gacagatagc gcagcacgtt ctccatgaag
    17761 ttcttcatgt catccgggtc gctgttgagc gtacactgtc cgtctttatt aacgcccccg
    17821 ttccagctgt aaccgttcgg gcaacgcagg atgctgttgt agtgtgggtt accgataacc
    17881 atcagcttgc catcgcccac ttgccccagc gagataaacg gcaggttgaa ggtggcggtt
    17941 tcgcgtgtca cgttctctgg tcgcacaatg gaaggcgctt cagtaatgta cgccagctca
    18001 tttttatccc aggcgcgttt ttcgccaaac gccagccagt agtttttatc attacgcgcc
    18061 atcagaatcg ggaaggccgc gttggagata ttcaccaccg cctgaccgcg cgcattaccc
    18121 gtgctgccat agaagttagt agagtcatgg aatacatgga acttgctgac agatttgtag
    18181 ttcgtatcca cgccccacag cttgttgata acgccctgaa tctggccgtc attaacattg
    18241 cgcgtcgtca gcgagaacca gcgagcctcg ttacaaccgt cggttttcgc acaaatcgcg
    18301 gtatcgatct ctttggcctg acccgtctta aactgctcga taaattcgtt tggcagatta
    18361 acaacttgct caccttcatc cagcgtcgca ccgttggata acgagagatt gataatctcg
    18421 ttgatcacgt tgggatattc ggcaaagacc ttgcgtacat catccggaac aacacgggta
    18481 ttattttgcc cggtcgtcga atagcgatga ataagctgat cgatattcgc cccgcgaact
    18541 tcatcaccca gttcagtcag cgcaatggtc gacttattgc cgcgcactga acccagttca
    18601 aaggtatcga taccaaagga gatggtttcg ccccagctaa aagaaaattc accattttcc
    18661 cctgtcacgc cacggcctga attggtgtag tagttgacgc cagcaacacc atatccctgg
    18721 ctatccacca ggcgaccttc tgagagaatg atttccgaag gttgatactg ataaaactgt
    18781 tccgcgttag ccgacacgaa ggaggcgttc agatccggtt ttgttcccgg agtcgtgacg
    18841 gggactaccg gtgaagtatg agtggatggc gctttatcag tcgcggcatt gttttccacc
    18901 tcttcattga ccagcttttt gaactcttcc ggtgccagat cgatttgctt atacagcgag
    18961 tcgaaacgtt tactctcgat caccgaggag aactccaggc acacctgttc tgtattcgcc
    19021 gggcaactgt tcatggaggt gaccagcgag agcgcattgc ttttcttgtt gtcggaaccc
    19081 gccagttctt gcgcgtcctc aagactaaac gacacttttt caaccgcacg caggctacgc
    19141 gcagcttctg actgggtgtc gaaggtagca attgtcgtgt tgttccctgc cacacaggtg
    19201 actttgtcgc ctggtgtaaa ggtaaagcca tcgctggatt catcattaca ggtgatatca
    19261 ccagttaccc gcaggcttcc gcccagggtc agataacccg ttttcgtagg aacaggttct
    19321 ggctccggtt ctggtgttgg ctcaggatca ggtgtcggtt ccggcgtagg ttctgggtcc
    19381 ggcgttggct caggcgtcgg ctccgggttt ggtgttggat caggtttcac ttccggcaaa
    19441 gaccctgttc cagaatctac aggcggcgta tcggaggaag atccggagcc accgccatca
    19501 caaccggcta acagggttgc actcaaaata gccgctaaaa gcgatttctt atatttaaat
    19561 t
//
LOCUS       DAZFWP020000094        19402 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFWP020000094 DAZFWP020000000
VERSION     DAZFWP020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.094"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(22..171)
                     /locus_tag="ACHK0Z_RS15845"
                     /old_locus_tag="pgaptmp_003169"
     CDS             complement(22..171)
                     /locus_tag="ACHK0Z_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503150.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            480..1619
                     /gene="wza"
                     /locus_tag="ACHK0Z_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             480..1619
                     /gene="wza"
                     /locus_tag="ACHK0Z_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU4503151.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKIHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1625..2068
                     /gene="wzb"
                     /locus_tag="ACHK0Z_RS15855"
                     /old_locus_tag="pgaptmp_003171"
     CDS             1625..2068
                     /gene="wzb"
                     /locus_tag="ACHK0Z_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU4503152.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2071..4233
                     /gene="wzc"
                     /locus_tag="ACHK0Z_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             2071..4233
                     /gene="wzc"
                     /locus_tag="ACHK0Z_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU4503153.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4411..5250
                     /gene="wcaA"
                     /locus_tag="ACHK0Z_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             4411..5250
                     /gene="wcaA"
                     /locus_tag="ACHK0Z_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU4503154.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5253..5741
                     /gene="wcaB"
                     /locus_tag="ACHK0Z_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             5253..5741
                     /gene="wcaB"
                     /locus_tag="ACHK0Z_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU4503155.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGDNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5738..6955
                     /gene="wcaC"
                     /locus_tag="ACHK0Z_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             5738..6955
                     /gene="wcaC"
                     /locus_tag="ACHK0Z_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU4503156.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            6930..8147
                     /gene="wcaD"
                     /locus_tag="ACHK0Z_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             6930..8147
                     /gene="wcaD"
                     /locus_tag="ACHK0Z_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU4503157.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWSAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8158..8904
                     /gene="wcaE"
                     /locus_tag="ACHK0Z_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             8158..8904
                     /gene="wcaE"
                     /locus_tag="ACHK0Z_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU4503158.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8920..9468
                     /gene="wcaF"
                     /locus_tag="ACHK0Z_RS15890"
                     /old_locus_tag="pgaptmp_003178"
     CDS             8920..9468
                     /gene="wcaF"
                     /locus_tag="ACHK0Z_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU4503159.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            9495..10616
                     /gene="gmd"
                     /locus_tag="ACHK0Z_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             9495..10616
                     /gene="gmd"
                     /locus_tag="ACHK0Z_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU4503160.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10619..11584
                     /gene="fcl"
                     /locus_tag="ACHK0Z_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10619..11584
                     /gene="fcl"
                     /locus_tag="ACHK0Z_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU4503161.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11587..12066
                     /gene="gmm"
                     /locus_tag="ACHK0Z_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11587..12066
                     /gene="gmm"
                     /locus_tag="ACHK0Z_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU4503162.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            12063..13286
                     /gene="wcaI"
                     /locus_tag="ACHK0Z_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             12063..13286
                     /gene="wcaI"
                     /locus_tag="ACHK0Z_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU4503163.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13289..14725
                     /gene="cpsB"
                     /locus_tag="ACHK0Z_RS15915"
                     /old_locus_tag="pgaptmp_003183"
     CDS             13289..14725
                     /gene="cpsB"
                     /locus_tag="ACHK0Z_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU4503164.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14918..16288
                     /gene="cpsG"
                     /locus_tag="ACHK0Z_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /gene_synonym="manB"
     CDS             14918..16288
                     /gene="cpsG"
                     /locus_tag="ACHK0Z_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU4503165.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16343..17737
                     /gene="wcaJ"
                     /locus_tag="ACHK0Z_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             16343..17737
                     /gene="wcaJ"
                     /locus_tag="ACHK0Z_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU4503166.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSMIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17739..19217
                     /gene="wzxC"
                     /locus_tag="ACHK0Z_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             17739..19217
                     /gene="wzxC"
                     /locus_tag="ACHK0Z_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU4503167.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLGVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         4671 a         4861 c         5342 g         4528 t
ORIGIN      
        1 gagacgcaat attaaatatt gtcaacctaa agaaactcct aaaaaccata ttgaatgaca
       61 cttaatataa ttcttaaaaa tagccaattg ccgaattgtt accttgcctg ctattccgtt
      121 agctgtaaca cttcctcctg cattattgga gagccaatat tcaattacca taatgtcacc
      181 ttatattcgg aagttaattt ccgcaataac cctttctcat caacgattgt atgcagagag
      241 ggtttgaaaa attaagctaa atcagtgtat tggtagctaa aaagccaggg gcggtagcgt
      301 gtctggatgc ctgaaagacc agtctgaatt attctgtcaa tagtctgcgg atgaaatcaa
      361 tttttttagg actgatgcca gttaaatttt tattcaatta actgcaaggt aaatgtgcat
      421 ggcgacattg ctattaatag tgcacaggat aattactctg ccaaagtgat aaataaacaa
      481 tgatgaaatc caaaatgaaa ttgatgccat tattggtgtc agtaaccttg ataagcggtt
      541 gcacagtact tccgggcagc aatatgtcga cgatgggcaa agacgtcatc aaacagcagg
      601 acgctgattt cgatctcgac aaaatggtga atgtttatcc gctgaccccg cgcctgattg
      661 accaattacg cccacgcccg aatgtagcgc gccccaatat gacgctggaa agtgagatcg
      721 cgaattacca gtatcgcgtc gggccgggag acgttcttaa tgtcaccgtc tgggatcacc
      781 cggaactcac cacgccagct ggtcagtacc gcagctccag cgacaccggc aactgggtac
      841 agcctgacgg cactatgttc tacccgtata tcggcaagat tcacgtcgtg gggaaaacgc
      901 tcgctgaaat ccgcagtgat attaccgggc gcttagcgac gtacatcgct gacccgcagg
      961 tggacgttaa tatcgccgcc ttccgctcac aaaaggccta tatctccggt caggtgaata
     1021 aatccggtca acaggcgatc accaacgtac cactgaccat tctcgatgcc atcaacgccg
     1081 caggtggcct gaccgacacc gctgactggc gcaacgtggt gctaacacac aatggtcgtg
     1141 aagaacgcat ttctttgcag gcgctgatgc aaaacggcga cctcaatcag aaccgactgc
     1201 tttaccccgg cgatattctc tacgtgccgc gtaatgatga tctgaaagta tttgtgatgg
     1261 gtgaagtgaa gaaacagagc accctgaaaa tggactttag cggcatgacc ctgacggaag
     1321 ccttgggtaa tgctgaaggc atcgacatga ccacctccaa cgccagcggc atctttgtca
     1381 ttcgtccgct gaaaggcgag ggcgggcgta acggcaagat tgccaatatc taccagctgg
     1441 atatgtccga tgcgacgtcg ctggtgatgg cgacagaatt ccgcctgcaa ccttatgacg
     1501 tggtgtatgt caccaccgcc ccggtttccc gctggaaccg tctgatcaat cagttgctgc
     1561 caactattag cggtgtccgt tacatgacgg atacagccag cgacattcat aactggtaat
     1621 cgtcatgttt aacaacattt tagttgtctg tgtcggcaat atttgccgtt ccccgacggc
     1681 ggaacgctta ctgcaacgtt atcacccgga actgaaagtg gagtccgctg gactcggcgc
     1741 gctggtcggt aagggcgctg accctactgc tatcagcgtc gccgcagaac atcaactgtc
     1801 tctggaaggt cactgtgccc gtcaaatcag ccgccgcctg tgtcgcaact acgacctgat
     1861 tttgaccatg gaaaagcgcc atatcgaacg cttatgcgag atggcacccg agatgcgcgg
     1921 caaagtgatg ctgtttggtc actgggataa cgaatgtgaa atccccgatc cgtatcgcaa
     1981 aagccgggaa acgtttgcag cggtgtacac attacttgaa cggtctgccc gccagtgggc
     2041 gcaggcattg aacgcagagc aggtataaga atgacagaaa aagtaaaaca acatgccgct
     2101 ccggtaacgg gcagtgatga aatcgatatt ggtcgcctgg tcggcaccgt cattgaagcg
     2161 cgctggtggg tgattggcat caccgccgta tttgccctct gtgccgtggt ttacactttc
     2221 ttcgccacgc cgatttatag tgccgacgca ctggtacaaa tcgagcaaag cagcggcaat
     2281 tcgttagtgc aggacattgg ttcggcatta gccaacaaac cgcctgcatc ggacgccgag
     2341 atccagttga ttcgttcgcg tctggtgctt ggtaaaacgg tggacgatct cgacctcgat
     2401 attgcagtga gcaaaaacac gttcccgatt ttcggggcgg gctgggatcg cctgatggga
     2461 cgccagaacg agacggtgaa agtgactacc tttaaccgcc cgaaagagat ggcggatcag
     2521 gtgtttacgc ttaatgtgct ggatgacaaa aactacaccc tgagcagtga tggcggcttt
     2581 agcgcccgtg gacaagcggg ccagatgctg aaaaaagaag gcgtcacgct gatggttgaa
     2641 gccattcacg cccgcccggg cagtgagttt accgtcacca aatactccac gctggggatg
     2701 atcaatcaac tgcaaaacag cctgacggta acggagaacg gcaaagacgc aggcgtactg
     2761 agcctgactt ataccggtga agatcgcgaa cagatccgcg acattcttaa tagcatcgcc
     2821 cgtaactatc aggaacaaaa tattgagcgc aaatcggcgg aagcgtcgaa aagcctcgct
     2881 ttcctcgcgc aacagttacc ggaagtacgt agccgccttg atgttgccga aaacaaactg
     2941 aatgccttcc gtcaggataa agattctgtt gatctgccgc tggaagcgaa agcggtgctc
     3001 gattcgatgg tgaacatcga cgcccagttg aacgaactga cctttaaaga ggcggaaatc
     3061 tccaagctgt acaccaaagt tcacccggcg tatcgcacgc tgctggagaa acggcaggcg
     3121 ctggaagacg aaaaagccaa acttaacggt cgcgtaacgg cgatgccgaa aacccagcag
     3181 gaaattgtcc gtctgacccg cgatgtcgag tctgggcagc aggtctatat gcaactgctg
     3241 aataaagagc aggagctgaa aatcaccgaa gccagcaccg tcggcgatgt gcgcattgtt
     3301 gacccggcaa tcacccagcc tggcgtgctg aaaccgaaga aaggactgat tatcctcggc
     3361 gcaattatcc tcggcctgat gctctctatc gtgggtgtgc tgctgcgctc gttgtttaat
     3421 cgcggcatcg aaagcccgca ggtgctggaa gaacatggta tcagcgtcta tgccagcatc
     3481 ccgttgtcgg aatggcagaa agcgcgcgat agcgtcaaaa ccatcaaagg gattaaacgc
     3541 tataaacaga gccagctact ggcggtgggg aatccaaccg atctggcgat tgaagccatc
     3601 cgcagtctgc gtaccagtct gcacttcgcg atgatgcagg cgcagaacaa tgtgttgatg
     3661 atgacagggg tgagcccgtc aatcggtaaa acctttgtct gcgccaacct ggcggcggtg
     3721 atcagtcaga ccaataaacg ggtgttgttg atcgactgcg atatgcgcaa aggctacacc
     3781 cacgagctgt tgggtaccaa taacgtcaat ggcctgtcgg aaattctgat tggccagggc
     3841 gatattacta ccgcagctaa accgacctct attgccaaat ttgacctgat cccgcgcggt
     3901 caggtaccgc caaatccttc tgaactgttg atgagcgaac gctttgccga actggtgaac
     3961 tgggcgagta aaaactatga cctggtgttg attgatacgc cgccgattct ggcagtgacc
     4021 gatgcggcaa ttgttggtcg tcatgttgga accacgttaa tggtggcgcg ttatgcggtc
     4081 aacacattga aagaagtgga aaccagtctg agccgctttg agcaaaatgg tattccggtg
     4141 aaaggggtga ttctgaactc catcttccgc cgcgccagcg cgtatcagga ttatggctat
     4201 tacgaatacg aatataagtc ggatgcgaaa taacgacatc cgctgtcgga tgcgatgctg
     4261 acgcatctta tccgacctac cttcacgccg catccggcaa gcaaaccagc tccgtaggcc
     4321 ggataaggcg ctcacgccgc atccggcaac agaacccgta aacaagctta taaaccggca
     4381 gaacatctcg acactccggg agaacaacct atgaaagaca atccgctgat ttcaatctat
     4441 atgccgacct ggaaccgcca acaactggcg attcgggcga taaaatcggt cctgcgccag
     4501 gactacagca actgggagat gatcatcgtg gatgattgct ccacctcctg ggaacaactg
     4561 caacagtacg tcactgccct caacgatccg cgtattacct acattcacaa cgacattaac
     4621 agcggggcgt gcgcggtacg taaccaggcg attatgctgg cgcaagggga atacattacc
     4681 gggatcgatg acgatgatga atggacgccc aaccgcctga gcgtcttcct cgcccataaa
     4741 cagcaactgg tcactcacgc ctttttgtac gctaacgact acgtttgcca gggcgaagtc
     4801 tattcccaac cagcaagctt accactgtat cccaaatcgc cgtactcacg ccgcctgttc
     4861 tataaacgca atatcattgg caatcaggtt tttacctggg cgtggcgttt caaagagtgc
     4921 ttgttcgata ccgaactgaa agcagcacag gattacgaca tcttcctgcg gatggtggtg
     4981 gagtatggcg aaccgtggaa agtggaagag gcgacgcaga tcctgcatat caatcacggt
     5041 gaaatgcaga tcacctcgtc accgaaaaaa ttctccgggt acttccactt ttaccgcaag
     5101 cacaaagaca aattcgaccg tgccagtaaa aaatatcagc tgtttaccct ctaccagatc
     5161 cgcaataaac gcatgacctg gcgcactttg ctgacgctgc tgtcggtgcg taacggcaaa
     5221 cgtctggctg atggtattcg ggggcgctaa agatgctgga agatctgcgc gccaacagct
     5281 ggagtttacg cccgtgctgc atggttcttg cctatcgcgt tgctcatttt tgttcggtgt
     5341 ggcgcaaaaa gaacgtcctc aacaatctgt gggcggcccc gctgctggtg ctgtatcgca
     5401 ttatcaccga atgctttttc ggttatgaaa tccaggcagc cgcaaccatt ggccgccgct
     5461 ttactatcca tcacggttac gccgtagtga tcaataaaaa cgtggttgcg ggggatgatt
     5521 tcaccattcg ccacggcgtc actatcggca atcggggtgc cgataacatg gcatgtccac
     5581 acattggcaa cggcgtcgaa ctcggtgcca acgtcattat tcttggtgat atcacgcttg
     5641 gtgacaacgt caccgtgggc gcgggcagcg tggtgctcga ctctgtcccg gacaacgcgc
     5701 tggtggtggg agaaaaagcg cgagtgaagg taattaaatg aatattttgc aatttaatgt
     5761 gcgactggcg gaaggcgggg cagcaggtgt ggcgttagat ctccaccagc gtgcgctgca
     5821 acaggggctg gcgtcacatt ttgtctacgg ttacggcaaa ggcggcaaag agagcgtcag
     5881 ccatcagaac tatccgcagg tcatcaaaca tacgccgcgg atgaccgcga tggcgaatat
     5941 tgccctgttt cgtctgttta atcgcgatct gtttggcaat ttcaatgagt tatatcgcac
     6001 cattactcgc acaccgggtc cggtggtcct gcattttcat gtgctgcaca gctactggct
     6061 aaatcttaag agcgtggtgc gcttttgcga aaaagtgaaa aaccacaaac cggatatcac
     6121 tctggtctgg acgctgcacg atcactggag cgttaccgga cgctgcgcct ttaccgacgg
     6181 ttgcgaaggc tggaaaacgg gctgccagaa atgcccgacc ttaaataact atccgccggt
     6241 gaagattgat cgcgcgcatc agctggtggc gggcaaacgc cagttattcc gcgagatgct
     6301 ggcgctgggc tgtcagttta tttcccccag ccagcatgtg gctgacgctt tcaatagtct
     6361 gtacgggcca gggcgttgcc ggattatcaa taatggcatt gatatggcaa ccgaagcgat
     6421 tctggcagat ctgcctccgg tgcgtgaaac ccagggcaag ccgaaaatcg cggtggtggc
     6481 gcatgacttg cgttacgacg gcaagactaa ccagcaactg gtgcgtgaga tgatggcgct
     6541 gggcgacaaa atcgaactgc atacctttgg taagttctcg ccgttcaccg ctggcaacgt
     6601 ggttaatcac ggctttgaaa ctgacaagcg caagttgatg agcgcgctca atcagatgga
     6661 tgcgctggta ttcagttctc gcgtcgataa ctacccgctg attttgtgtg aggcgttatc
     6721 aattggcgtg ccggtgattg ccacccatag cgacgcggca cgggaagtgt tgcaaaaatc
     6781 cggcggtaaa accgtcgacg aagaagaggt gctgcaactg gtccagttaa gcaaaccgga
     6841 aatcgcgcag gcgatatttg gtaccacgct ggctgagttc agccaacgca gccgcgccac
     6901 ctacagtgga caacagatgc tggaggagta tgtcaacttc tatcagaatc tgtagctacc
     6961 tgctgctgcc gctgatctac ctgctggtta acgtcaaaat cgcccagctt ggcgaaagtt
     7021 tccccatcac tatcgtcact tttttacctg tcttgttact gctgttttta gaacgcatca
     7081 gcgttaaaaa attgatgatt gccttaggga ttggtgcggg actcacggcg ttcaactatc
     7141 tgtttggtca gtcgttggat gccagtaaat acgtcacatc aaccatgctg tttgtctata
     7201 ttgtgatcat tattggcatg gtgtggagta ttcgctttaa aacgatttcg ccacacaacc
     7261 atcgcaagat attacgtttc ttttatctgg tggtcgggct ggtggtggcg ctggcggcgg
     7321 tggagatggc gcaaattatc ctcaccggtg gcagcagtat tatggagtcg atttcgaaat
     7381 atctgattta cagcaacagc tatgtactga atttcattaa attcggcggc aagcgcacga
     7441 cagcacttta tttcgaaccg gcatttttcg ctctggcatt aatctcaatt tggctcagca
     7501 tcaaacagtt tggtatcaaa acgcctaaaa ccgatgctat gattctcgca gggataatat
     7561 tatccggatc gttttcaggg gttatgacct ttatcctgtt ttatttgctg gagtgggcat
     7621 ttcaatatct gaataaagag gcgattaaga aaaagttacc gttggcattg atttctctgg
     7681 ctgtattcct ggttggtgtg gtaatcgcgt ttccttatat ttccacccgt ctgggcgatt
     7741 taggtacgga aggatcgtca tcgtattatc gtattgtcgg tccgctggtg atggtcggtt
     7801 attctttgac ccatattgac ggtgtagtca gatttggctc actttatgaa tacgtcgcat
     7861 cattcggaat atttaacggt gcggatgtcg gaaaaaccat agacaatggt ttgtatctgc
     7921 tgattattta tttttcctgg tccgcagtgt ttttatcgct gtggtacatg gggaaagtga
     7981 taaaaatgat gatcaacgct tttggtgata accgcaattt tcgcgtgcaa ttatatcttt
     8041 ttactccggt atcgctgttt tttaccggtt cgatatttag cccggaatat gcatttttaa
     8101 tcgtctgtcc gtttattttg cgaaaagcgt taaatattac gaggtaagaa taagaacatg
     8161 ttgcttagca taatcactgt cgcgtttcgt aacctcgaag ggatagtcaa aacacatgcc
     8221 tcgctggcgc atctggcgca ggcggaagat atcagcttcg aatggattgt tgtcgatggc
     8281 ggttccaatg acggcacccg tgagtatctg gaaaatctca atggtatcta taacctacgc
     8341 tttgtcagcg agccagataa cggtatctac gacgccatga acaaaggcat tacgatggca
     8401 caaggcaagt tcgcgttgtt tctcaattcg ggcgatattt ttcatcagga tgccgcaaat
     8461 tttgtccgta agttaaaaat gcaaaaagat aacatgatga tcaccggcga tgcgctgctg
     8521 gattttggcg acgggcataa aattaaacgt agcgccaaac cgggctggta tatttatcac
     8581 agcctgcccg ccagtcatca ggcgatattt ttccctgtat ccggcttgaa aaaatggcgt
     8641 tatgacctgg aatataaagt ttcttccgac tacgcgctgg cagccaaaat gtataaagcc
     8701 ggttatgcat ttaaaaaact caatggcctg gtgtctgaat tttccatggg tggggtatct
     8761 accaccaata atatggaatt gtgtgctgac gcgaaaaaag tccaacgaca aatattacat
     8821 gtacctggct tttgggctga attatcctgg catttacgcc aacgtactac ctcaaagacg
     8881 aaagccttat ataacaaagt ctgaatataa ggagaaccaa tgcaagattt aagcggtttc
     8941 tcggtgccga aagggttccg gggcggcaac gctattaaag tgcaattatg gtgggcagta
     9001 caggcaacaa tatttgcctg gtcgccacaa gtattgtatc gctggcgggc atttttatta
     9061 cgtttattcg gcgcaaaaat aggaaaaaac gtagttattc gtccgtcagt aaaaattacc
     9121 tatccgtgga aattaacctt aggtgattac gcgtgggtcg gcgatgacgt caatttatat
     9181 accctcggtg aaataaccat tggcgcacat tcggtgatat cgcaaaaaag ttatttatgc
     9241 accggtagcc acgaccatgc aagtcaacat ttcaccatta atgctacgcc tattgtgatt
     9301 ggcgagaaat gctggctggc aaccgatgtt tttgttgccc ctggcgtcac gattggcgac
     9361 ggcaccgtcg tgggtgcacg aagcagtgtt tttaaaacgc ttccggcaaa tgtggtttgc
     9421 cgggggaatc ccgcagtggt gatacgcgaa cgcgttgaaa ctgaataaat tcaaaaaata
     9481 cagaggaata agacatgtca aaagtcgctc tcatcaccgg tgtaaccgga caagacggtt
     9541 cttacctggc agagtttctg ctggaaaaag gttacgaggt gcatggtatt aagcgtcgtg
     9601 catcgtcatt caacaccgag cgcgtggatc acatttatca ggatccgcac acctgcaacc
     9661 cgaaattcca tctgcattat ggcgacctga gtgatacctc caacctgaca cgcattttgc
     9721 gtgaagtgca gccggatgaa gtttacaacc tgggcgcaat gagccacgtt gcggtttctt
     9781 ttgagtcacc ggaatatacc gcagacgtcg atgcgatggg tacgctgcgc ctgctcgagg
     9841 cgatccgctt cctcggtctg gaaaagaaaa cccgtttcta tcaggcttcc acctctgaac
     9901 tgtatggtct ggtgcaggaa attccgcaga aagaaaccac gccgttctac ccacgctctc
     9961 cgtatgctgt cgccaaactg tacgcctact ggatcaccgt taactaccgc gaatcctacg
    10021 gcatgtacgc ctgtaacggt attctcttca accatgaatc cccgcgccgc ggcgaaacct
    10081 tcgttacccg caaaatcacc cgcgcaatcg ccaatatcgc ccaggggctg gagtcgtgcc
    10141 tgtacctcgg caatatggat tccctgcgtg actggggcca tgccaaagac tacgtaaaaa
    10201 tgcagtggat gatgctgcaa caggaacagc cggaagattt cgttatcgcg accggcgttc
    10261 agtactccgt gcgtcagttc gtggaaatgg cggcagcaca gctgggcatc aaactgcgct
    10321 ttgaaggcac gggcgttgaa gagaagggca ttgtggtttc cgtcaccggg catgacgcgc
    10381 cgggcgttaa accgggcgat gtgattatcg ccgttgaccc gcgttacttc cgtccggcag
    10441 aagttgaaac gctgctcggc gacccgacca aagcgcacga aaaactgggc tggaaaccgg
    10501 aaatcaccct cagagagatg gtgtctgaaa tggtggctaa tgacctcgaa gcggcgaaaa
    10561 aacactctct gctgaaatct cacggctacg acgtggcgat cgcgctggag tcataagcat
    10621 gagtaaacaa cgcattttta tcgctggcca tcgtgggatg gtcggttctg ccatcaggcg
    10681 gcagctcgaa cagcgcggtg atgtggaact ggtattacgc acccgcgacg agctgaacct
    10741 gctggacagc cgcgcggtgc atgatttctt tgccagcgaa cgcattgacc aggtctatct
    10801 ggcggcggcg aaagtgggcg gcattgttgc caacaacacc tatccggcgg atttcatcta
    10861 ccagaacatg atgattgaga gcaacatcat tcacgccgcg catcagaacg acgtgaacaa
    10921 actgctgttt cttggatcgt cctgtatcta cccgaaactg gcaaaacagc cgatggcaga
    10981 aagcgagtta ttgcagggca cgctggagcc gaccaacgag ccttacgcca ttgccaaaat
    11041 cgccgggatc aaactgtgcg aatcatacaa ccgccagtac ggacgcgatt accgctcagt
    11101 catgccgacc aacctgtatg gcccgcatga caacttccac ccgagtaatt cgcatgtgat
    11161 ccctgcattg ctgcgtcgct tccacgaggc gacggcacag aatgcaccgg acgtggtggt
    11221 atggggcagc ggtacaccga tgcgtgaatt cctgcacgtc gatgatatgg cggcggcgag
    11281 cattcatgtc atggagctgg cgcacgaagt ctggctggag aacacccagc cgatgctgtc
    11341 gcacattaac gtcggcacgg gcgttgactg cactatccgc gagctggcgc aaaccatcgc
    11401 caaagtggtg ggttacaaag gtcgggtggt ttttgatgcc agcaaaccgg atggtacgcc
    11461 gcgcaaactg ctggatgtga cgcgcctgca tcagcttggc tggtatcacg aaatctcact
    11521 ggaagcgggg cttgccagca cttaccagtg gttccttgag aatcaagacc gctttcgggg
    11581 gtaatgatgt ttttacgtca ggaagacttt gccacggtag tgcgctccac cccgcttgtc
    11641 tctctcgact ttattgtcga gaacagtcgc ggcgagtttc tgcttggcaa aagaaccaac
    11701 cgcccggcgc agggttactg gtttgtgccg ggagggcgcg tgcagaaaga cgaaacgctg
    11761 gaagccgcat tcgagcggct gacgatggcg gaactggggc tgcgtctgcc gataacagca
    11821 ggccagtttt acggtgtctg gcagcacttt tatgacgata acttctccgg cacggatttc
    11881 accactcact atgtggtgct cggttttcgc ttcagagtcg ctgaagaaga tctgatactg
    11941 ccggatgagc agcatgacga ttaccgctgg ctgacgccgg acgcgctgct cgccagtaac
    12001 gatgttcacg ctaacagccg cgcctatttt ctcgctgaga agcgtgccgg agtacccgga
    12061 ttatgaaaat actggtctac ggcattaact actcgccgga gttaaccggt atcggcaaat
    12121 acaccggcga gatggtggaa tggctggcgg cacaaggtca tgaggtgcgg gttattaccg
    12181 caccgcctta ctacccgcag tggcaggtgg gcgagaacta ttccgcctgg cggtacaaac
    12241 gagaagaggg ggccgccacg gtgtggcgct gcccgctgta cgtgccaaaa cagccgagca
    12301 ccctgaaacg cctgttacat ctcggcagtt ttgccgtcag cagtttcttt cctctgatgg
    12361 cgcaacgtcg ctggaagccg gatcgcatta tcggcgtggt gccaacgctg ttttgcacgc
    12421 cgggaatgcg cctgctggcg aaactttctg gtgcgcgtac cgtgctgcat attcaggatt
    12481 acgaagtgga cgccatgctg gggctgggcc ttgccggaaa aggcaaaggc agcaaagtgg
    12541 cacagctggc aacggcgttc gaacgtagcg gactgcataa cgtcgataac gtctccacga
    12601 tttcgcgttc gatgatgaat aaagccatcg aaaaaggcgt ggcggcggaa aacgtcatct
    12661 tcttccccaa ctggtcggaa attgcccgtt ttcagcatgt tgcagatgcc gatgttgatg
    12721 cccttcgtaa ccagcttgac ctgccggata acaaaaaaat cattctttac tccggcaata
    12781 ttggtgaaaa gcaggggctg gaaaacgtta ttgaagctgc cgatcgtctg cgcgatgaac
    12841 cgctgatttt tgccattgtc gggcagggcg gcggcaaagc gcggctggaa aaaatggcgc
    12901 agcagcgtgg actgcgcaac atgcaatttt tcccgctgca atcgtatgac gctttacccg
    12961 cactgctgaa gatgggcgat tgccatctgg tggtgcaaaa acgcggcgcg gcagatgccg
    13021 tattgccgtc gaaactgacc aatattctgg cggtaggcgg taacgcggtg attaccgcgg
    13081 aggcccacac agaactgggg cagctttgcg aaacctttcc gggcattgcg gtttgcgtag
    13141 aaccggaatc ggtcgaggcg ctggtggcgg ggatccgtca ggcgctcctg ctgcccaaac
    13201 acaacacggt ggcacgtgaa tatgccgaac gcacactcga taaagagaac gtgttacgtc
    13261 aatttataaa tgatattcgg ggataattat ggcgcagtcg aaactctatc cagttgtgat
    13321 ggcaggtggc tctggtagcc gcttatggcc gctttcccgc gtactttacc ccaagcagtt
    13381 tttatgcctg aaaggcgatc tcaccatgct gcaaaccacc atctgccgcc tgaacggcgt
    13441 ggagtgcgaa agcccggtgg tgatttgcaa tgagcagcac cgctttattg tcgcggaaca
    13501 gctgcgtcaa ctgaacaaac ttaccgagaa cattattctc gaaccggcag ggcgaaacac
    13561 ggcacctgcc attgcgctgg cggcgctggc ggcaacacgt catagcccgg agagcgaccc
    13621 gttaatgctg gtattggcgg cggatcatgt gattgccgat gaagacgcgt tccgtgccgc
    13681 cgtacgtaat gccatgccgt atgccgaagc gggcaagctg gtgaccttcg gcattgtgcc
    13741 ggatctacca gaaaccggtt atggctatat tcgtcgcggt gaagtgtctg cgggtgagca
    13801 ggatacggtg gcctttgaag tggcgcagtt tgtcgaaaaa ccgaatctgg aaaccgccca
    13861 ggcctatgtg gcaagcggcg aatattactg gaacagcggc atgttcctgt tccgcgccgg
    13921 acgctatctc gaagaactga aaaaatatcg tccggatatt ctcgatgcct gtgaaaaagc
    13981 gatgagcgcc gtcgatccgg atctcgattt tattcgtgtg gatgaagaag cgtttctcgc
    14041 ctgcccggaa gagtcggtgg attacgcggt catggaacgt acggcagatg ccgttgtggt
    14101 gccgatggat gcgggctgga gcgatgtcgg ctcctggtcc tcattatggg aaattagcgc
    14161 ccacaccgcc gagggcaacg tttgccacgg cgatgtgatt aatcacaaaa ctgaaaacag
    14221 ctatgtgtac gccgaatctg gcctcgtaac caccgtcggg gtgaaagatc tggtagtggt
    14281 gcagaccaaa gatgcggtgc tgattgccga ccgtaacgcg gtgcaggatg tgaaaaaagt
    14341 ggtcgagcag atcaaagccg atggtcgcca tgagcatcgg gtgcatcgcg aggtctatcg
    14401 cccgtggggc aaatatgact ctatcgacgc gggcgaccgc taccaggtga aacgcatcac
    14461 cgtgaaaccg ggcgagggct tgtcggtaca gatgcaccat caccgcgcgg aacactgggt
    14521 agtggtcgcg ggaacggcaa aagtcactat tgacggtgat atcaaactgc ttggtgaaaa
    14581 cgagtccatt tatattccgc tgggggcgac gcactgcctg gaaaacccgg ggaaaattcc
    14641 gctcgattta attgaagtgc gctccggctc ttatctcgaa gaggatgatg tggtgcgctt
    14701 cgcggatcgc tacggacggg tgtaaacgtc gcatcaggcg ctgtttgtcg gatgcggcgt
    14761 gaacgcctta tccgacctac ggttcggatt tgtaggcctg ataagacgcg gcagcgtcgc
    14821 atcaggcaat gaatgcgaaa ccgagatgta aataacgaca aaaacaaaat tggccgtttc
    14881 ggtcagggcc aactattgcc tgaaaaaggg taacgacatg aaaaaattaa cctgctttaa
    14941 agcctatgat attcgcggga aattaggcga agaactgaat gaagatattg cctggcgcat
    15001 tgggcgtgcc tatggcgaat ttctcaaacc gaaaaccatt gtgttaggcg gtgatgtccg
    15061 tctcaccagc gaaaccttaa aactggcgct ggcaaaaggt ttacaggatg cgggcgttga
    15121 cgtgctggat atcggtatgt ccggcaccga agagatctat ttcgccacgt tccatctcgg
    15181 cgtggatggc ggcattgaag ttaccgccag ccataatccg atggattata acggcatgaa
    15241 gctggttcgc gagggggctc gcccgatcag cggagatacc ggactgcgcg acgtccagcg
    15301 tctggctgaa gccaacgact ttcctcccgt cgatgaaacc aaacgcggtc gctatcagca
    15361 aatcaacctg cgtgacgctt acgttgatca cctgttcggt tatatcaatg tcaaaaacct
    15421 cacgccgctc aagctggtga tcaactccgg gaacggcgca gcgggtccgg tggtggacgc
    15481 cattgaagcc cgctttaaag cccttggcgc acccgtggaa ttaatcaaag tgcacaacac
    15541 gccggacggc aatttcccca acggtattcc taacccgcta ctgccggaat gccgcgacga
    15601 cacccgcaat gcggtcatca aacacggcgc ggatatgggc attgcctttg atggcgattt
    15661 tgaccgctgc ttcctgtttg acgaaaaagg gcagtttatc gaaggctact acattgtcgg
    15721 cctgctggca gaagcgttcc tcgaaaaaaa tcccggcgcg aagatcatcc acgatccacg
    15781 tctctcctgg aataccgttg atgtggtgac tgccgctggc ggcacgccgg tgatgtcgaa
    15841 aaccggacac gcctttatta aagaacgtat gcgcaaggaa gacgccatct acggtggcga
    15901 aatgagcgct caccattact tccgtgattt cgcttactgc gacagcggca tgatcccgtg
    15961 gctgctggtc gccgaactgg tgtgcctgaa aggaaaaacg ctgggcgaac tggtgcgcga
    16021 ccggatggct gcgtttccgg caagcggtga gatcaacagc aaactggcgc aacccgttga
    16081 ggcgattaat cgcgtcgaac agcattttag ccgtgaggcg ctggcggtgg atcgtaccga
    16141 tggcatcagc atgacctttg ccgactggcg ctttaacctg cgcacctcca acaccgaacc
    16201 ggtggtgcgg ttgaatgtgg aatcgcgcgg tgatgtgccg ctgatggaag cgcgaacgcg
    16261 aactctgctg acgttgctga atgagtaatg tcggatcttc ccttacccca ctgcgggtaa
    16321 ggggctaata acaggaacaa cgatgacaaa tctaaaaaag cgcgagcgag cgaaaaccaa
    16381 tgcatcgtta atctctatgg tgcaacgctt ttcagatatc accatcatgt ttgccggact
    16441 atggctggtt tgcgaagtca gcggactgtc attcctctac atgcacctgt tggtggcgct
    16501 gattacgctg gtggtgttcc agatgctggg cggcatcacc gatttttatc gctcatggcg
    16561 cggtgttcgg gcagcgacag aatttgccct gttgctacaa aactggacct taagcatgat
    16621 tttcagcgcc ggactggtgg cgttcaacaa tgatttcgac acgcaactga aaatctggct
    16681 ggcgtggtat gggctgacca gtatcggact ggtggtttgc cgttcatgta ttcgtattgg
    16741 ggcgggctgg ctgcgtaatc atggctataa caagcgcatg gtcgccgtag cgggggattt
    16801 agccgccgga caaatgctga tggagagctt ccgtaatcag ccgtggttag ggtttgaagt
    16861 ggtgggcgtt taccacgacc cgaaaccggg cggcgtttct aacgactggg cgggcaatct
    16921 gcaacagctg gtcgaggatg ctaaagcggg caagattcat aacgtctata tcgcgatgca
    16981 aatgtgcgac ggcgcgcgag tgaaaaaact ggtccatcaa ctggcggaca ccacctgttc
    17041 ggtgctgctg atccccgatg tctttacctt caatattctc cattcacgtc tcgaagagat
    17101 gaacggcgtt ccggtggtgc cgctgtatga cacgccgctt tccggggtta accgcctgct
    17161 aaaacgtgcg gaagacattg tgctggcgac gcttatcctg ctgctgatct ccccggtgct
    17221 gtgctgcatt gcgctggcgg tgaaactcag ttcacctggg ccggttattt tccgccagac
    17281 tcgctacggc atggatggca agccgatcaa agtgtggaag ttccgttcca tgaaagtgat
    17341 ggagaacgac aaagtggtga cccaggcgac gcagaacgat ccgcgcgtca ctaaagtggg
    17401 gaactttctg cgccgcacct cgctggacga attgccgcag tttatcaatg tgctgaccgg
    17461 ggggatgtcg attgtcggtc cacgtccaca cgcggtggcg cataacgaac agtatcgcca
    17521 gctcattgaa ggctacatgc tgcgtcataa agtgaaaccc ggcattaccg gctgggcgca
    17581 gattaatggc tggcgcggcg aaaccgacac gctggagaaa atggaaaaac gcgtcgagtt
    17641 cgaccttgag tacatccgcg aatggagcgt ctggttcgat atcaaaatcg ttttcctgac
    17701 ggtattcaaa ggcttcgtta acaaagcggc atattgatat gagcttacgt gaaaaaacca
    17761 tcagcggcgc gaagtggtcg gcgatagcca cggtgatcat catcggcctc gggctggtgc
    17821 agatgaccgt gctggcgcgg attatcgaca accaccagtt cggtttgctt accgtgtcgc
    17881 tggtgatcat cgcgctggca gatacgcttt ctgacttcgg tatcgctaac tcgattattc
    17941 agcgaaaaga aatcagccac cttgaactca ccacgctgta ctggctgaac gtcgggctgg
    18001 ggcttgtggt gtgcgtggcg gtgtttttgt tgagtgatgc cattggcgac gtgctgaata
    18061 acccggacct ggcaccgttg attaaaacat tgtcgctggc gtttgtggtg atcccccacg
    18121 ggcaacagtt ccgcgcgttg atgcagaagg agctggagtt caacaaaatc ggcatgatcg
    18181 aaaccagcgc ggtactggcg ggcttcactt ttacggtggt tagcgcccat ttctggccgc
    18241 tggcgatgac cgcgatcctc ggttatctgg tcaatagtgc agtaagaacg ctgctgtttg
    18301 gttactttgg ccgcaaaatt tatcgccccg gtctgcattt ctcgctggcg tcggtggcac
    18361 cgaacttacg ttttggtgcc tggctgacgg cggacagcat catcaactat ctcaatacca
    18421 acctttcaac gctggtgctg gcgcgtattc tcggcgcggg cgtggcaggg ggatacaacc
    18481 tggcgtacaa cgtggccgtt gtgccgccga tgaagctgaa cccaatcatt acccgcgtgt
    18541 tgtttccggc atttgccaaa attcaggacg acaccgaaaa gctgcgcgtt aacttctaca
    18601 agctgttgtc ggtagtgggg attatcaact ttccggcgct gctggggctg atggtggtgt
    18661 cgaataactt tgtaccgctg gtctttggtg agaagtggaa cagcattatt ccggtgctgc
    18721 aattgctgtg tgtggtgggt ctgctgcgct ccgtaggtaa cccgattggt tcgctgctga
    18781 tggcgaaagc gcgggtcgat atcagcttta aattcaacgt attcaaaaca tttctgttta
    18841 ttccggcgat tgttataggt gggcagatgg cgggcgcgat cggcgtcacg cttggcttcc
    18901 tgctggtgca aattatcaac accattctga gttacttcgt gatgattaaa ccggttcttg
    18961 gttccagtta tcgtcagtac atcctgagtt tgtggctgcc gttttacctc tcgctgccga
    19021 cgctgggggt cagttatgcg ctgggcattg tgctgaaagg gcaattggcg ctggggatgc
    19081 tgctggcggt gcaaatagcc gcgggcgtgc tggcgtttgt ggtgatgatt gtgctgtcgc
    19141 gccatccgct ggtggtggaa gtgaagcgtc agttttgtcg cagcgaaaaa atgaaaatgc
    19201 ttttacgggc ggggtgagtt gctgttttcg gttccgacaa cggttgccgg atgcggcgta
    19261 aaacgcctta tccgtcctac ggttctgtgc tcgggacctg taggcctgat aagacgcgtc
    19321 agcgtcgcat caggcactga atgccggatg cggcgtgaac gccttatccg gcctacggtt
    19381 cagggcttgg atcttgtagg cc
//
LOCUS       DAZFWP020000095        19220 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFWP020000095 DAZFWP020000000
VERSION     DAZFWP020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.095"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            184..2574
                     /gene="gcd"
                     /locus_tag="ACHK0Z_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     CDS             184..2574
                     /gene="gcd"
                     /locus_tag="ACHK0Z_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU4503168.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2621..4171)
                     /gene="cueO"
                     /locus_tag="ACHK0Z_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             complement(2621..4171)
                     /gene="cueO"
                     /locus_tag="ACHK0Z_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU4503169.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4337..4684
                     /gene="yacC"
                     /locus_tag="ACHK0Z_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             4337..4684
                     /gene="yacC"
                     /locus_tag="ACHK0Z_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU4503170.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4790..5656
                     /gene="speE"
                     /locus_tag="ACHK0Z_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             4790..5656
                     /gene="speE"
                     /locus_tag="ACHK0Z_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU4503171.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5672..6466
                     /gene="speD"
                     /locus_tag="ACHK0Z_RS15955"
                     /old_locus_tag="pgaptmp_003191"
     CDS             5672..6466
                     /gene="speD"
                     /locus_tag="ACHK0Z_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU4503172.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6504..6866)
                     /gene="yacL"
                     /locus_tag="ACHK0Z_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             complement(6504..6866)
                     /gene="yacL"
                     /locus_tag="ACHK0Z_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU4503173.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVIVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7042..9639)
                     /gene="acnB"
                     /locus_tag="ACHK0Z_RS15965"
                     /old_locus_tag="pgaptmp_003193"
     CDS             complement(7042..9639)
                     /gene="acnB"
                     /locus_tag="ACHK0Z_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU4503174.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAVVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9994..11751
                     /gene="yacH"
                     /locus_tag="ACHK0Z_RS15970"
                     /old_locus_tag="pgaptmp_003194"
     CDS             9994..11751
                     /gene="yacH"
                     /locus_tag="ACHK0Z_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU4503175.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPFITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(11822..13246)
                     /gene="lpdA"
                     /locus_tag="ACHK0Z_RS15975"
                     /old_locus_tag="pgaptmp_003195"
     CDS             complement(11822..13246)
                     /gene="lpdA"
                     /locus_tag="ACHK0Z_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU4503176.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13571..15463)
                     /gene="aceF"
                     /locus_tag="ACHK0Z_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             complement(13571..15463)
                     /gene="aceF"
                     /locus_tag="ACHK0Z_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU4503177.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKIAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15478..18141)
                     /gene="aceE"
                     /locus_tag="ACHK0Z_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             complement(15478..18141)
                     /gene="aceE"
                     /locus_tag="ACHK0Z_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU4503178.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18302..19066)
                     /gene="pdhR"
                     /locus_tag="ACHK0Z_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             complement(18302..19066)
                     /gene="pdhR"
                     /locus_tag="ACHK0Z_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU4503179.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4439 a         5325 c         4816 g         4640 t
ORIGIN      
        1 aacaaatcac ttcgcacggg caggttttgg caccgcaaca aagcgcaaga cacgcagtat
       61 accagtaaaa ctattcattc gcggaatctg ttaataaagc tcgaattgtg atgacgatca
      121 cacatgttaa aacctataat taattgctac taaaatatta atgaattgaa atggtgtctc
      181 tttatggcaa ttaacaatac aggctcgcga cgattactcg tcacgctaac agcccttttt
      241 gcagcgcttt gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc
      301 tcctggtact accctatcgc tggccttgtg atgctcggcg tcgcctggat gctgtggcgc
      361 agtaaacgtg ccgcgctttg gctgtacgca gccctgctgc tcggcaccat gatttggggc
      421 gtctgggaag ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc
      481 ttcggcatct ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc
      541 gcagttgccg cactggtggt ggcactgctg attagcggtg gtatcctgac ctgggccgga
      601 tttaacgatc cgcaggaaat caacggcacc ttaagcgcca atgccacacc tgcggaagct
      661 atctcacccg ttgccgatca ggactggcct gcctatggtc gcaatcagga aggtcaacgc
      721 ttttcacctc tgaaacaaat taacgccgat aacgtccata atctgaaaga agcctgggtg
      781 ttccgtactg gcgatgtgaa gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg
      841 ccgattaaag tgggcgacac cctttacctg tgtaccgctc accagcgcct gtttgcgctc
      901 gatgccgcca gcggcaaaga gaaatggcat tacgatcctg agctgaaaac taacgagtct
      961 ttccagcacg taacctgccg tggcgtctct tatcatgaag ccaaagcaga aacggcttcg
     1021 ccggaagtga tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgactg
     1081 attgcgatta acgctgaaaa cggcaaactg tgcgaaacct tcgccaataa aggcgtgctc
     1141 aatctgcaaa gcaatatgcc agacaccaaa ccgggtctgt atgaaccgac ttcgccaccg
     1201 attatcaccg ataaaaccat cgtgatggcc ggttcagtta ccgataactt ctcaacccgc
     1261 gaaacgtctg gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt
     1321 gatcccggcg cgaaagatcc gaacgcaatc ccgtctgacg aacacacctt tacctttaac
     1381 tcgccaaact cctgggcacc agcggcctat gacgcgaagc tggatctggt ctatctgccg
     1441 atgggcgtga ccacgccgga tatctggggc ggtaaccgca caccggaaca ggaacgttat
     1501 gccagctcga ttctggcgct gaatgccact accgggaaac tggcgtggag ctaccagacc
     1561 gttcaccacg acctgtggga catggatctt ccggcacagc cgacgctggc ggacatcacc
     1621 gttaatggtc agaaagtgcc agttatttac gctccggcga aaaccggcaa catttttgtg
     1681 ctcgatcgtc gcaatggcga actggtggtt ccggccccgg aaaaaccggt gccgcaaggt
     1741 gctgcgaaag gcgattacgt tactccgacc cagccgttct ctgaactgag cttccgtccg
     1801 aagaaagatt tgagcggtgc ggatatgtgg ggagccacca tgtttgacca actggtgtgc
     1861 cgcgtgatgt tccaccagat gcgctatgaa ggcattttca cgccgccatc tgaacagggc
     1921 acgctggtct tcccgggtaa cctgggaatg tttgaatggg gcgggatttc cgttgatcca
     1981 aatcgtgaag tggcaattgc caacccaatg gcactgccgt ttgtttcgaa actgatccca
     2041 cgcggtccgg gtaacccgat ggagcagccg aaagatgcca aaggcacggg tacggaatcc
     2101 ggcattcagc cacagtacgg tgtaccgtat ggtgtcacgc tcaacccgtt cctctcaccg
     2161 tttggtctgc cgtgtaaaca gccagcatgg ggttatatct cggcgctgga tctgaaaacc
     2221 aatgaagtgg tgtggaagaa acgtattggt acgccgcaag acagtatgcc gttcccgatg
     2281 cctgtaccgg tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat ctccacggcg
     2341 ggtaacgtgc tgtttatcgc tgctacggca gataactacc tgcgcgctta caacatgagc
     2401 aacggtgaaa aactgtggca gggccgttta ccagcgggtg gtcaggcgac gccaatgacc
     2461 tatgaagtga atggcaagca gtatgtggtg atctccgcag gcggtcacgg ttcatttggt
     2521 acgaagatgg gcgactatat tgtggcttat gcgctgccgg atgatgtgaa ataaccataa
     2581 tgcccggaga gatccgggca tatttccgaa tacggtcttt ttataccgta aaccctaaca
     2641 tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca tgttctttcg
     2701 gtgcgttgtg attaaacttc accagcactt cactgacatt gccttctacc ttaacggtat
     2761 ctttccagcc cgcgcgatga gccgctggcg gtttgccatt ttctgacaag atacggaact
     2821 gggtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag ataacccaac
     2881 gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac gcctgaccgt
     2941 tgattttatt ggcatggtgg aaatcgaact tcccgccatg gttcatatga ttcatattgc
     3001 cgtgccccat atggcccatc atctggctat gatccatccc ggccatcgcc tgatcgccat
     3061 atttctccat cagcatctgc atccccatca tatcgagcat cgggtccata gaaagttgca
     3121 gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt aatgtgtctg
     3181 gcaaagcacc ggaggcacta atagcaatcg gctgaatccg cattaccgga tgcggcttgt
     3241 caaacggcgc aatcgccatc cccatctggc tgaccggtag cgtcaccagg tcaaagggtt
     3301 tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc ggcagctcgc
     3361 tcaccttcac cggttcaggt agcagaccac cgtcgctggc aatcacatac aacgggcgat
     3421 tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa cgcaggcgca
     3481 gccaaccacg tggggcagcg tgttgtgggt agattgcacc gttggtcagc agcgtatcgc
     3541 caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc ccatcagcgt
     3601 taaatttttt atcctgaaca atcaccggaa catcatcgat accccactgt tttggcagca
     3661 ttaatttcag gatctcgtca tcttcaatta ccaccagccc agccaacccc atcgccacct
     3721 gtcgcccggt cttgccatgt tgatgtggat ggaaccagca agtagcggca ggttgatcaa
     3781 cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgcggg ccgccgtcga
     3841 cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc agttggttat
     3901 agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc agcagattgc
     3961 cgttatagcc ccaggtagtt gcagttttct cgccaaaggt agactgacct gcgccaatag
     4021 ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt aacgttgggc
     4081 gttccgccgc aaagactgcg cggctccaca gcggcaaggc tgaagccaca cccagcgcga
     4141 cggaatattt taagaaatca cgacgttgca tagttatttc cttattctta agcaggcgaa
     4201 acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc gcagacacaa
     4261 tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa
     4321 gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca gcctgatggc
     4381 cgtttgcgca aacagttacg cgctcagcga gtctgaagcc gaagatatgg ccgatttaac
     4441 ggcagttttt gtctttctga agaacgattg tggttaccag aacttaccta acgggcaaat
     4501 tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta attacgacac
     4561 cttcgacatg aaagccctcg gtgaagacag ctaccgcgat ctcagcggca ttggcattcc
     4621 cgtcgctaaa aagtgcaaag ccctggcccg cgattcctta agcctgcttg cctacgtcaa
     4681 ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca atgttagcta
     4741 ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca tggccgaaaa
     4801 aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg tagataacgt
     4861 tctgtatcat gaaaagaccg atcaccaaga tctgatcatt tttgagaacg ctgcatttgg
     4921 tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt ttatctatca
     4981 tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg tgctgattat
     5041 cggcggcggc gacggtgcca tgctgcgtga agtaacccga cataaaaacg ttgagtcaat
     5101 cacgatggtg gaaatcgatg cgggtgtcgt atcgttctgc cgtcagtatc tacccaacca
     5161 taacgccggt agctacgacg atccgcgctt taagttggtg atcgacgatg gcgtcaattt
     5221 cgttaatcaa accagccaga cctttgatgt cattatctcc gactgcaccg atcctatcgg
     5281 tcccggcgaa agccttttca cttcggcatt ttatgaaggc tgcaaacgtt gcctgaatcc
     5341 tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag aagccatcga
     5401 cagccatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg cggcgattcc
     5461 gacctattac ggcggtatca tgacttttgc atgggcgaca gataacgacg ccttacgcca
     5521 tctctcaacc gaaattattc aggcgcgttt tctcgcttct ggcctgaaat gccgttatta
     5581 caatccggca atccatacgg cagcttttgc cttacctcag tatctgcaag acgcactggc
     5641 ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc atggctttaa
     5701 taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca aaactgccga
     5761 agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc gtctgaccga
     5821 aatcctgtca gaaacctgtt ccattatcgg ggccaatatt cttaacatcg cccgccagga
     5881 ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg tcgacccgaa
     5941 acttatcgac aaaacagaac accccggccc actgccagaa acggtcgttg cccatctcga
     6001 taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg gtttatgtac
     6061 cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga aggcgctgaa
     6121 ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg tgcgcggttt
     6181 tactcgcgac attaacggta tgaaacactt tatcgaccat gagattaatt cgattcagaa
     6241 ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaatg tctatcagga
     6301 aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact acatgttcca
     6361 caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg cgctgtggaa
     6421 agaaatgcgc gagatttatt acgggcgcaa tatgccagct gtttaacgtc tcgggcggag
     6481 ctcccaggct ccgccagatt tatttacttc tgctgcacga aattacggta agccgccacg
     6541 acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta gtagttcatc
     6601 ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaacaat cacctcttca
     6661 ccgtccatcc acagagtgta ctcatgccct gcccgctgcc aggaacgttc gctacctttc
     6721 agtgcgtgcg ccgcttgttc cacttcatca agcaaggcca ggttttcttt cacctcttca
     6781 ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac aactccggta
     6841 atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa tgcgccgaaa
     6901 tcgcattgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag cggaacggcg
     6961 atggtttaga aataaaaaaa tgcccgggaa tctcattcac cgggcattgt gtcgtttatg
     7021 cgcagcgcgt gcgcttactt attacaccgc agtctggaaa atcaccccat cggctttttc
     7081 ggtgtactga gaaagctggt tgaagttcag ataacgataa gtatcaacgg cagttttatc
     7141 tacttgcgcc acgtaggtct ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc
     7201 aacaaccgcc agttccgcag aagccaggaa gacattcgcg ccagtaccca gacggttcgg
     7261 gaagttacgg gtagaggtgg aaaccaccgt cgcaccgtct gctacacgcg cctggttacc
     7321 catacacagg gaacagccag ggatctcgat acgcgcacca ctcttaccga agacgctgta
     7381 gtagccttct tcagtcaact gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg
     7441 ggtcggcagt tggcctttgt gcgcatccag cagtttaccc gcagcacgga agtgaccgat
     7501 gttggtcatg caggaaccga taaatacttc gtcgatcttc tcaccctgta ccgcagacag
     7561 cggacgcgcg tcatccgggt cgttcggtgc acagaggatt ggctctttaa tatccgccag
     7621 atcgatgtcg atcactgccg cgtattcagc atctgcatcg gcttccagca gctcaggatt
     7681 cgccagccat ttttccatgc cctgaatacg acgttccagg gtgcgacgat cgccgtaacc
     7741 ttccgcgatc atccacttca gcaggacgat gttagagttc aggtattcga tgatcggttc
     7801 tttgttcagc ttgatggtac aaccagcggc agaacgctcg gcggacgcat cggtcagttc
     7861 aaatgcctgc tcaactttca gatccggcag accttcaatt tccaggatgc ggccagagaa
     7921 gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg catacagcgg
     7981 aatggcatgt accagatcgc gcagggtgat gcccggctgc attttgcctt tgaagcgcac
     8041 cagaacggat tccggcatat ccagcggcat tacgcctgtt gcggctgcaa atgccaccag
     8101 accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggaat caccaccggt
     8161 accgacggta tccggcagca gcatacggtt cagccaggag tgaatgacgc cgtcacccgg
     8221 acgcagcgac acaccgccac ggttcataat gaagtccggc agcgtgtggt gcgtgttcac
     8281 gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga
     8341 gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggccgg tggtgtcctg
     8401 ggaacctaca gaagtcattt tcggttcaca gtacgcgccc ggacgaatgc ctttcacgcc
     8461 acaggcacgg cctaccattt tttgcgccag cgagaagccg cgatcgctct cagcgacatc
     8521 tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt
     8581 caggccacgc ccgataatca gcggaatacg gccaccagca cgcacttcat caatcagcac
     8641 gtcggttttc agttcgaagg tcgccagcag ttcgccggtt tcgtggttac gcacttcacc
     8701 tttgtacggg taaacgtcaa tcacgtcgcc catgttcagg ttagagacgt cgacttcgat
     8761 tggcagtgca cccgcgtctt ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag
     8821 gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca taaaccacag
     8881 aacggagtta gtggcggatt tacgcgaaga acccgtaccc acaacgtcac cgacgtacgc
     8941 cagcgggaaa cctttctgtt gcagagcttc gatttgcttg atcggaccaa caacaccagg
     9001 ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat
     9061 atccgggcgt gaccacgcat ccggtgccgg agaaaggtca tcggtgttag tttcgccagt
     9121 gactttgaag acggtaacgg tcagtttttc agccagcgcc gggcgattca ggaaccattc
     9181 ggcatccgcc caggactgca taacctgctt cgcatattcg ttgcctgctt tcgctttctc
     9241 ttctacgtca tagaagttat cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat
     9301 aggtgccagt ttggcatcat ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg
     9361 catggtgccc agcagttcga tggctttttc cggagtcagc agaggggatt tggcttcgcc
     9421 tttcgcgata gcagccagga agcctgcttt gacataggcg gcttcatcga cgcctggggg
     9481 aacacggttg gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag
     9541 cagctctaca agtgcggcca tttggtttgc atccaggggt ttgggcgcaa tcccctcagc
     9601 ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc tcgctctcat
     9661 tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt tgtagggtaa
     9721 atgcccagta ccgcaacggg cagaatagca ggattttgac gatgtgttaa tctgtttaca
     9781 aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt gtttccctcc
     9841 gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc ccaagattag
     9901 tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg gtggcagttc
     9961 gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc gtttaaaccc
    10021 catgtgctgg cgctaatttg cagtgccggg ctttgtgccg cctctaccgg gctatatata
    10081 aaaagccgca cagtggaagc gcctgtggaa ccgcaatcga cacaacagac tgcgcctgac
    10141 atcaccgcag ttacgcttcc tgcaacggtt tccgcaccgc ccgtaacgcc cgccgtcgtc
    10201 aaatccgcat tcagcactgc acaaatcgat caatgggttg cgcctgtcgc gctgtacccc
    10261 gattctctgc tttcacaagt gttaatggca tcaacctatc cggcaaacgt tgctcaagca
    10321 gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca ggcggtatct
    10381 gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt gatggcattg
    10441 atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag
    10501 gacgtgatgg actcggtaca gcgattgcgg caactggcgc aacaaaccgg ttcgctgaag
    10561 tcatcaaccg aacaaaaagt tattaccaca acgaagaaag ctgtaccggt aaaacagaca
    10621 gtcacggcac ccgtcatacc atccaatacc gttttaactg ccagcccctt cattacagag
    10681 cctgcaacaa ccgtcatttc cattgagccc gccaatcctg atgtggtcta tattcccaac
    10741 tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc ggtttatctg
    10801 ccaccaccag ccggagaacc gtttattgac agctttgtgc gcggattcgg ctatagcatg
    10861 ggtgttgcta ccacgtacgc actattcagc agcatcgact gggatgacga cgatcatgac
    10921 catcatcatc atgacgatga tgattatcat caccacgatg gcggtcatcg tgacggtaat
    10981 ggctggcaac acaacggcga caacatcaat atcgacgtca acaatttcaa ccgtatcacc
    11041 ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta ccgtaatggt
    11101 gtgccctatc atgatcagga tatggcaaag cggtttcatc aaaccgatgt caacggcgga
    11161 atgagcgcca cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt
    11221 cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca acgtcaggca
    11281 gcggcacagc ggtttaatga agctgaaaac tatgggagct atgacgactt ccgcgacttc
    11341 agccgtcgcc aaccactgac ccagcaacaa aaggacgccg ctcgtcagcg ttatcagtcg
    11401 gcctcgcctg agcagcgcca ggcagtccgc gagaaaatgc agactaaccc acagatccag
    11461 cagcgaagag acgcagcgcg tgagcgtatt cagtcagcct cgcctgagca gcgccaggtg
    11521 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    11581 cgtgttcaat cagcatcacc tgaacaacgt caggtttttc gggaaaaagt tcaggagagc
    11641 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    11701 gcagtacgcg aacgtctctc tgagcgcgga gcaaggcgac aggaaaggta aattgcagac
    11761 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaca
    11821 attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    11881 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt
    11941 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    12001 taccgacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    12061 taccgtctgc gcagtcggaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    12121 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    12181 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg
    12241 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    12301 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    12361 ggatgaaacc acggtcgtcc acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    12421 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt
    12481 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    12541 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    12601 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    12661 cgtggtatac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    12721 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    12781 gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcattg tcgaagttga
    12841 tcacggtttt gccgttctca ccttcaactt ccagggtgtt agccccggtg aatttaccca
    12901 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    12961 gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga
    13021 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    13081 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    13141 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    13201 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    13261 atttatctcc ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc
    13321 ctcaccccag tcacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg
    13381 cacccagaaa tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc
    13441 cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct
    13501 attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg
    13561 gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa
    13621 tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca
    13681 gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa
    13741 cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc
    13801 cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt
    13861 cacgcgcttt cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt
    13921 tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt
    13981 tgatgtattt cttcagggtc agacgctgac cgtcttccga caacgaactg ttgaagcgag
    14041 gcatctgctc aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca
    14101 catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccagctcgg
    14161 tgatatcggt tttgtcgaag tgagtaacat gcgggatcat cacccagtta cggctcaggt
    14221 tcgcaccaga aattttctgg atacggccca gttccacttc ttcgatttca ccaaacttgc
    14281 tgaagtccac cttcggccac ggcagcatgc ctgggatacc gccgccagtc gccgccggag
    14341 ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac
    14401 gacctttacg gccagtgccc ttcactttcg ccaggttaac gccaaactcg cgtgccagac
    14461 ggcggatcag cggagtcgcg tgaacgtaag cgtcgttttc tgcaaattca gatttgcctt
    14521 ccgctttcgc agccggtgct gctgccggag cttcagcttt tgctgccgga gccggcgctg
    14581 ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa
    14641 tcagcgagcc agttttcact ttatcgccaa cgttgacttt cagttccttc acgacgcctg
    14701 caaacggagc cgggacttcc atagaagctt tatcgccttc tacggtgatc agtgactgtt
    14761 cagcggcaat tttgtcgccc actttcacca tcacttcggt cacttcaact tcgtcaccgc
    14821 cgatatccgg aacgttaact tctttcacgc cagccgctgg tgcaggggcc gctgccggag
    14881 ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact tcgaagacca
    14941 taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct ttcacggtgc
    15001 cagcaaacgg agccggaact tccatagaag ctttatcgcc ttctacggtg atcagcgact
    15061 gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca acttcgtcgc
    15121 tgccgatatc cggaacgtta acgtcttttg ccgctgcagc cgctggtgct gctgccggag
    15181 ctgtttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat
    15241 cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact ttgatctctt
    15301 taacgatacc cgcctgcgga gacggaactt ccatagaggc tttgtcgcct tctacggtga
    15361 tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg gtgatttcaa
    15421 cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt ttacctctta
    15481 cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg cgtcagcaac
    15541 cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcta caaccacgta
    15601 ggaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc
    15661 atcagtaccc agtacgcggt agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa
    15721 cagtttcata tagtcggtag atgccactgc cggagcgtcg ttcatcacct gagcgatata
    15781 cggaacgcgc ggagtttcca gcgggtgcag catgttccag cgttcacaat cctgaccatc
    15841 tcgcgccagt tcggtgaagg aggtcacgct ataaacgtca gaacctacgc cgtaatcttt
    15901 cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc ccagcagctg
    15961 aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac ggataccttc
    16021 ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta
    16081 gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca taatgacagc
    16141 aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca gcgactgaat
    16201 gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac gaccggaagt
    16261 accgccgatc aggaagccac gcgcttgctg gtcgccagcc gcccagcaca gatcgccgat
    16321 acgctggaag ccgaacatcg agtaatagat gtagaacgga atcatcggca gattgttggt
    16381 gctgtaagag gtcgccgctg ccagccagga acaacctgcg cccaactcgt tgatcccttc
    16441 ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg
    16501 ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac cttccatacc
    16561 gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg acttgttctt
    16621 cagcattacg ttcagggcac gaacgaaagc gatagtggta gagatctctt tgctctgctc
    16681 ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg tgaagttcgg
    16741 ctgacggctt ggcagataac cgtgcagttt ctgacgctgt gcgtgcagat aggtatgctc
    16801 ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat cagacaccgg
    16861 cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct taacctggtg
    16921 cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag
    16981 gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt agattttctt
    17041 cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt ctgcaaccag
    17101 tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac catctttcga
    17161 tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca gtttaccgct
    17221 ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga tcacgttcca
    17281 gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac cggtgaccgg
    17341 gccgtcaaga cgctgcaggt tacagttgat aacgaagacc aggttatcca gtttttcacg
    17401 ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt cgccgaggaa
    17461 cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat atttcaggaa
    17521 tttagcctgg taaatagcac caatcggccc cagacccata gatacggtcg ggaactgcca
    17581 gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt gaacttcctg
    17641 acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac gagcgtacac
    17701 gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc
    17761 acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga aggacgccat
    17821 atggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga tggcgttcca
    17881 gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact ccggttgttc
    17941 ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta cgtttacacc
    18001 gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct caacaccttc
    18061 ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc
    18121 atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt gagttttctg
    18181 gaacctgtcc cattgaactc tcgccggaaa gctcaataag acaggttcta cgtttagttg
    18241 ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac cagaaaaatc
    18301 actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc tcttcacgac
    18361 ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt
    18421 cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg
    18481 gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc tgggccaaca
    18541 tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct tcggtgacgg
    18601 caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca
    18661 gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta ctacgcagcg
    18721 cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg
    18781 actcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc cataggctgc
    18841 tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt
    18901 gaatcgcctc acgcaaggag ggacgggaga cgtcaaactg ttttgccagt tcgcgttccg
    18961 gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc agttgctgct
    19021 caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt tcctgtctta
    19081 agccacttgc cgaagtcaat tggtcttacc aatttcatat ctgtgacgct aaagtaacaa
    19141 agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg cacattttaa
    19201 caacttgaca tatataacgt
//
LOCUS       DAZFWP020000096        18612 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFWP020000096 DAZFWP020000000
VERSION     DAZFWP020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.096"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(22..645)
                     /gene="rsmG"
                     /locus_tag="ACHK0Z_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     CDS             complement(22..645)
                     /gene="rsmG"
                     /locus_tag="ACHK0Z_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU4503180.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(709..2598)
                     /gene="mnmG"
                     /locus_tag="ACHK0Z_RS16000"
                     /old_locus_tag="pgaptmp_003200"
     CDS             complement(709..2598)
                     /gene="mnmG"
                     /locus_tag="ACHK0Z_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU4503181.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2977..3420)
                     /gene="mioC"
                     /locus_tag="ACHK0Z_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     CDS             complement(2977..3420)
                     /gene="mioC"
                     /locus_tag="ACHK0Z_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU4503182.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3510..3968)
                     /gene="asnC"
                     /locus_tag="ACHK0Z_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             complement(3510..3968)
                     /gene="asnC"
                     /locus_tag="ACHK0Z_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU4503183.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4120..5112
                     /gene="asnA"
                     /locus_tag="ACHK0Z_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             4120..5112
                     /gene="asnA"
                     /locus_tag="ACHK0Z_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU4503184.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5117..6568)
                     /gene="viaA"
                     /locus_tag="ACHK0Z_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             complement(5117..6568)
                     /gene="viaA"
                     /locus_tag="ACHK0Z_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU4503185.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6562..8058)
                     /gene="ravA"
                     /locus_tag="ACHK0Z_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             complement(6562..8058)
                     /gene="ravA"
                     /locus_tag="ACHK0Z_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU4503186.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEIDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8281..10149
                     /gene="kup"
                     /locus_tag="ACHK0Z_RS16030"
                     /old_locus_tag="pgaptmp_003206"
     CDS             8281..10149
                     /gene="kup"
                     /locus_tag="ACHK0Z_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU4503187.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10316..10735
                     /gene="rbsD"
                     /locus_tag="ACHK0Z_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             10316..10735
                     /gene="rbsD"
                     /locus_tag="ACHK0Z_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU4503188.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10743..12248
                     /gene="rbsA"
                     /locus_tag="ACHK0Z_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             10743..12248
                     /gene="rbsA"
                     /locus_tag="ACHK0Z_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU4503189.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGLGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12253..13218
                     /gene="rbsC"
                     /locus_tag="ACHK0Z_RS16045"
                     /old_locus_tag="pgaptmp_003209"
     CDS             12253..13218
                     /gene="rbsC"
                     /locus_tag="ACHK0Z_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4503190.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13243..14133
                     /gene="rbsB"
                     /locus_tag="ACHK0Z_RS16050"
                     /old_locus_tag="pgaptmp_003210"
     CDS             13243..14133
                     /gene="rbsB"
                     /locus_tag="ACHK0Z_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU4503191.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14259..15188
                     /gene="rbsK"
                     /locus_tag="ACHK0Z_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             14259..15188
                     /gene="rbsK"
                     /locus_tag="ACHK0Z_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4503192.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALTPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15192..16184
                     /gene="rbsR"
                     /locus_tag="ACHK0Z_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             15192..16184
                     /gene="rbsR"
                     /locus_tag="ACHK0Z_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU4503193.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16150..17577)
                     /gene="mdtD"
                     /locus_tag="ACHK0Z_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             complement(16150..17577)
                     /gene="mdtD"
                     /locus_tag="ACHK0Z_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU4503194.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17600..18292)
                     /gene="yieP"
                     /locus_tag="ACHK0Z_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             complement(17600..18292)
                     /gene="yieP"
                     /locus_tag="ACHK0Z_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU4503195.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4496 a         4614 c         4854 g         4648 t
ORIGIN      
        1 ttatgatttt tttgataaaa attaaatttt atttgcttta atcaccacca gatggcgttc
       61 gccatccagg gcaggaacct gaagtttaac cactgattca acctgatatt cttcgggcaa
      121 caaagcgatt tcatcttccg gcatttgccc tttcagcgcg tagaaacggc cttgctcacc
      181 aggaagatgg tgacaccagc tcaccatatc gttcagagag gcaaaagcgc ggctaattac
      241 gccatcaaat ggcggctctg aaggaaactc ttctaccctg ctctgtactg gctcaatatt
      301 ctccagttta agctcatgtt gcacctgacg aaggaaacgc acgcgtttac caaggctatc
      361 caacagagtg aaatgggctt caggacgcac gatagagagt ggaatgccag gcagtccagg
      421 accggtgccg acatcgataa accgttcacc ttgcagatac ggtgccacca caatgctatc
      481 gagaatatgg cgtaccagca tctcattagg atcgcggacc gaagtcaggt tgtacgcttt
      541 gttccattta tgcagcatat tcacgtaggc aataagctgg tttttctggt gatcggtaag
      601 cgaaatacct gcgtctttca gcagtaagga gagtttgttg agcacggtga ttacctgttc
      661 ttgatgcgtt gcctggtaag cgggtgctta ccaggcattt ttaatgcgtt atgcgctacg
      721 acgcagcata ccctgttttt tcagccacac cagcagaatg gagatggccg caggcgtgac
      781 gccagaaata cgtgaagcct ggccgataga ggccggtttg tgatcgttaa gtttggcgat
      841 cacttcgtta gaaagaccgg atacctggcg gtaatccagt gtcgctggta gcagggtgtt
      901 ctcgttacgc agctgctttt cgatctcatc ttgctggcgc gcgatataac cttcgtattt
      961 aacctgaatc tcaacctgtt ccgccgcctg ttcgtctgtc aacgcagggg caaacggcgt
     1021 cagcgtggtt aatttttcat aagtcatttc cggacgacgc agcagatctt caccactggc
     1081 ttcacgggaa agcggcgcag tcaggtgagc attcacttcg gctgcagctt ccgccgacgg
     1141 ggttacccag gtcgatttca gacgctgacg ctcacgctcg atattctcaa gtttctcgtt
     1201 aaagcgcgcc caacgttcgt catccaccag gcccagttca cgaccgattt cagtcaaacg
     1261 cagatccgca ttatcttcgc gcagcatcag acgatattct gcgcgcgaag taaacatacg
     1321 atacggttct ttggttccta aagtgcacag gtcatcaacc agtacgccga gataggcctg
     1381 agaacgcgcc ggagcccaac cttctttgtc agcagacaga cgggcagcgt taagaccggc
     1441 cagcaaacct tgcgcagcgg cttcttcgta accggtagtg ccgttaatct gaccagcaaa
     1501 gaacagcccc tggataaact tgctctccag cgtcggtttc aggtcgcgag gatcgaagaa
     1561 gtcatactca atggcataac ccggacgcac gatcttcgcg ttttccatcc cctgcataga
     1621 gcggacgatt tgcatctgca catcgaacgg caggctggtg gagataccgt tcggataaat
     1681 ttcgttagaa gtcagccctt ccggttcaag gaagatctga tgctgatttc tgtcggcgaa
     1741 gcgcatgact ttgtcttcga tcgacgggca gtagcgtggg ccgacacctt cgatcacccc
     1801 tgcatacatt gggctacgat cgaggttact gcggatcaca tcatgggttt tctcgttggt
     1861 atgagtgata taacacggca cctgctgggg atgctgggac gcattgccca taaacgagaa
     1921 taccggcatt gggttatcgc catgctgttg cgccagcaca ctaaagtcga tagttcgagc
     1981 atcaatacgc ggtggtgtcc cggttttcag acgaccaacg cgcagcggca gttcacgcaa
     2041 acggcgagaa agcggaatgg acggcggatc accagcacgg ccaccgctgt aattatccag
     2101 accgatatga attttaccgt cgaggaacgt cccaacggtg agcacgacgg ctttggcacg
     2161 gaacttcagt cccatttggg taacagcacc gaccacgcga tcgttttcga caataagatc
     2221 ttcaaccgcc tgctggaaga tcatcaggtt cggttggttc tccagcgccg tacgtaccgc
     2281 ctgacggtag agcacacgat ccgcctgagc tcgggtagcg cgaaccgccg gtcctttgct
     2341 tgcgtttagt atcctaaact ggatacccgc ttgatcgatc gctttcgcca tcagaccgcc
     2401 gagtgcatcc acttctttta ccagatgtcc cttcccaata ccgccgatcg ccgggttgca
     2461 gctcatctgc cccagagtgt cgatattgtg tgtcaaaagc agagtctgtt gacccatacg
     2521 cgccgcggcc atcgcggcct cggtgcctgc atgacccccg ccaatgatga tgacgtcaaa
     2581 aggatccgga taaaacatgg tgattgcctc gcataacgcg gtatgaaaat ggattgaagc
     2641 ccgggccgtg gattctactc aactttgtcg gcttgagaaa gacctgggat cctgggtatt
     2701 aaaaagaaga tctatttatt tagagatctg ttctattgtg atctcttatt aggatcgcac
     2761 tgccctgtgg ataacaagga tccggctttt aagatcaaca acctggaaag gatcattaac
     2821 tgtgaatgat cggtgatcct ggaccgtata agctgggatc agaatgaggg gttatacaca
     2881 actcaaaaac tgaacaaccg ttgttccttg gataactacc ggttgatcca agcttcctga
     2941 cagagttatc cacagtagat cgcacgatct gtatacttat ttgagtaaat taatccacga
     3001 tcccagccat tcttctgccg gatcttccgg aatgtcgtga tcaagaatgt tgatcttcag
     3061 tgtttcgcct gtctgttttg caccggaatt tttgagttct gcctcgagtt tatcgatagc
     3121 cccacaaaag gtgtcatatt cacgactacc aataccgatt gcgccaaagc ggaccgcaga
     3181 aagatcgggc ttctgttcct gcaatgcttc atagaaagga gaaaggttgt ccggaatatc
     3241 tccggcaccg tgggtggagc tgataatcag ccagatcccc gaggcagata aatcttccaa
     3301 cagcggaccg tgcagcgttt cggtggtaaa acccgcctct tccagctttt cagccaggtg
     3361 ttctgctaca tattcggcac cgccgagggt gctgccgctg ataagagtga tatctgccat
     3421 aaaccgccac ctttattaag agtggcgtat tgtacgctgt gaacgcgttg ggatctacct
     3481 gtggaaaagt atgggattaa aaaagccgat cagggcttga tagtacgcat gatcgggttt
     3541 tgcaggacga tcaatgtctc ggtggactga atttcatcaa ttgtttggat cttgttgata
     3601 agtacatgct ggagagcgtc gatcgaacgg cacattactt ttataaagat gctgtagtgg
     3661 ccggttgtgt aataggcttc agtgacttca tcaaggcttt ccagctttgc cagcgcggaa
     3721 gggtagtctt tggcgctctt taatataatg ccgataaagc agcctacgtc ataaccgagc
     3781 tgcttcgggc tgacatcaat acgcgccccg gtaatgatcc ccgcctgctt cattttctct
     3841 actcgaacgt gaatcgtccc cggactgacg ccaaattgtt tcgccagttc ggcgtaagcg
     3901 gtgcgcgcat tgcccattaa tgcttccagg atgccacggt ccagattgtc gatcagataa
     3961 ttttccatag gattttctta tgcagattga tgattcattc tattttagcc ttctttttta
     4021 atgaatcaaa agtgtgttag gctttttatt gaatgattat tgcatgtgtg tcggtttttg
     4081 ttgcttaatc ataagcaaca ggacgcagga gtataaaaaa tgaaaaccgc ttacattgcc
     4141 aaacaacgcc aaattagctt cgtgaaatct cacttttctc gccaactgga agaacgtctg
     4201 gggctgatcg aagtccaggc gccgattctt agccgtgtgg gggatggcac gcaggataac
     4261 ttgtcgggct gtgaaaaagc ggtgcaggta aaagtgaaag ctctgcctga tgcccagttc
     4321 gaagtggttc attcactggc gaagtggaaa cgtcagacct tagggcaaca cgacttcagc
     4381 gcgggcgaag ggctgtacac gcacatgaaa gcccttcgcc ccgatgaaga gcgtctttct
     4441 ccgttgcact cggtctatgt tgaccagtgg gactgggaac gcgtaatggg cgacggtgag
     4501 cgtcaattct cgactctgaa aagcacggta gaggcgatct gggcgggaat taaagcaacc
     4561 gaagctgcag ttagcgaaga gtttggtttg gcaccgttcc tgccggatca gatccacttc
     4621 gtacacagcc aggagttact gtctcgttat ccggatctcg atgccaaagg gcgtgagcgg
     4681 gcgattgcga aagatcttgg cgcggtattc ctcgtcggga ttggcggcaa gctgagtgat
     4741 ggtcatcgtc acgacgtgcg cgcaccggat tatgatgact ggagcacccc gtcagagctg
     4801 ggccatgcgg gtctgaacgg cgatattctg gtatggaacc ctgtactgga agatgcgttt
     4861 gagctttctt ccatggggat ccgcgtggat gccgacacgt tgaagcatca gttggcgctg
     4921 accggtgatg aagatcgtct gcagctggaa tggcatcagg cgctgctgcg cggtgaaatg
     4981 ccgcagacca tcggcggcgg tatcggccag tctcgtttga ccatgctgct gctgcaactg
     5041 ccgcatatcg gccaggttca gtgtggagta tggccagctg ctgttcgcga gagcgtccct
     5101 tctctgctgt aataatttat cgccgccagc gtctgagcag gcggcttcgc atcccggtat
     5161 caaagcgcca gatatgatcg aaaatgcgca tgatgccggg tttgccgtgt gccgacatcg
     5221 ccacggcatg aaagcgatgc tgatgtaccc gctgcagctc tttcacttta ctcgtcacgt
     5281 cgtcaggcaa ccgctgagcg ataaaatcag aaatcaccac cgcatcggca tcaaaccatt
     5341 ccctgctttg caagcgttcc ataatggcgc gaaaacaact ggcaagatcg gtgccgccac
     5401 gaaactgctg gcttaaaaaa cggattgctt gttcgatgcc ttgtgggcct gaaagctcat
     5461 aacggacgat ctcggtggaa aatagcataa tatagcagcg ccggttttct gcgagagcaa
     5521 tgcgcatcaa ggccaggcag aacgctttcg cacactgttc attaaagccg cccattgagc
     5581 cggaagtatc cacacagaca ataaacggcc cgcgcggctg ttcatcgtaa tctttatgta
     5641 ccaccggacg ttcgatcact ttttcacgcc acgactcacc gtgcaggcga taggtgagca
     5701 actgtttttc caccagccga cggtaaaact catactccag ttccgttatc cctagtgtcg
     5761 ccagttctgg cggcagaaga cgtaaaatat catcgctttg ttgcagacca tcaacctgct
     5821 caggaaccgt cgccggttcg cgcaccatgg tgcggaaggt ttccatctgc gcatcgttgc
     5881 gcggtattga tttggcttcc cgagaacgcc ccagctgctc tgccaggcgt ttcagttccg
     5941 gctgttcgtt aagaaattca ccgtatttca caatcaactg atagtcgcca cgtttaagct
     6001 gaccggcgct catatcccac agacgaccag ctgcagtatt gttatctgcg agaatcggtt
     6061 caagttgtcc gctcagcgtc atgcgttcct gaacttcact caacagttgt tcgcgttctt
     6121 cttctaatag ctgttgattt aacgtcgttg cttgcacgat cagacttaaa cgccaacgct
     6181 ggagaaaaag cgtgtgtaac gctgaagtga tcgtgctgtt agcatcaacc aactgtcggg
     6241 cttgttctgc ccatggagaa ttcagacgat gcagtaagtc caggatctgt ggtagctgca
     6301 caataaactg tggcgtggag aggagctggc tttgctgata gcacatcacc tcttcggtga
     6361 gttccggcgg gactcgggca tctttcagcc gactgcgcag cgcttcacgc cagcggggaa
     6421 catcatcagt gattgccgcc ttcaggcgtg ggaatttttc aaagaagacc gccagctgcg
     6481 gtgaggccag cagtgcgatg atcatctctt cgatcaatcc ctcttcgctg acggccagca
     6541 tcacattaag cgtatccagc gttagcattg ttgtgcctgg cgaatctgtg caccgacatc
     6601 ctgtaggctg gcttcaatgc gacctaacca gtcgccaggg ataaacagac atttttgctg
     6661 ttcgctgaat aaagcgtgtt gcttgcgcca gtcgctttca agctcttcca gttgttgttt
     6721 tatttcacca ggcagacctt cagccgatga accgggaagt gccagcgtac tgccttgtaa
     6781 acttacatcg cgaacgacaa gatgttgggc gctatcaatt tccagattca gtttctgggc
     6841 aaagccgata ccgttcagtt taccgcgaat ttcaccacct ttgctcagcc actgttccag
     6901 cgcgctacgc tcaaaggaga tatgaaccac ttccatatca tgcagtttta acggtttttg
     6961 cagcagtaga gtcagagtgg aagcagtaac gttaacagga agttgatact gctgacgacg
     7021 gctgaaaatg ccgcccagac gaattaccgt taaggctgtt ttatcgcttt gttgctgctg
     7081 gagttgcagg tgacgttgca caatcgcgcc aaggcgagtc aacatccctt gctgttgcca
     7141 ggcgtgaccg gtcatcaata catcaatttg ttgttgtatc aaattcaggc tttgcgcgtc
     7201 ataccacagg caatctttca gcaaaatgag atctaccggg gcaacagcac tgcgaccgct
     7261 aaaaaaggcg ctggcctgca ataatcggat cgcttttttc cagcgacgat ccgagacata
     7321 aggcgcatcc ggtaatttat ccagttgctg gcgcagcata aaaatcagct caaatacatg
     7381 atcgggcagc gtaatttcac caatctcttt ctgccagcgt tcatattctt catctgtgac
     7441 ctgcaaggca tcaggaaccg gattgtcgtt ttcatcctgt tgactggtca gcatggagcg
     7501 gaaattcgct ttatcctgca ctttatctaa ccacagacga atcagcatgc ggtcatataa
     7561 cgcttccaga ctgctgtctg cttccggcag ctcgttggag gccgccacca gcagacgcat
     7621 cgggattttt tctacgtgtg caccgttgcg gaactggcgc tcgttaatgg cggtgagcaa
     7681 ggtattaaga attgccgggc ccgctttcca gatctcatcc agaaagacaa tttctgcttc
     7741 cggcaggtaa ccgctggtta aacgttcata gcgcccttca tcttttagcg cctgaataga
     7801 aaggggacca aaaacttctt ccggcgtgga gaagcgggtc atcagatatt caaacgcgcg
     7861 ggcattctga aaggcgaatt ttaagcgccg ggcgatcaaa cttttggcaa tacctggcgg
     7921 gccaaggagg aacacacttt caccacttaa tgccgctaat aaacacaagc ggatggcgtg
     7981 gctacgttca taaagcccct tttccaacga actgctcagg cgggaaattc tttccgctaa
     8041 taaatgaggg tgagccataa tgaagtggcg tcctttcgtc aaaagttctg cgtaaattgc
     8101 gagtatagac gtttcttgct ggtggctaaa atagtctcaa agggggggta tttttctttg
     8161 agccaggtta atgtggccgc atttaggagt acgattttgc cgttaatcgt gcatactgtg
     8221 cgcttttttg tgggccaagg gactaagcac acatttcata tttcaacgaa agactagtct
     8281 atgagcactg ataataagca atcattgccc gcgattaccc tcgcggcgat tggagttgtc
     8341 tacggcgata ttggtaccag cccgttatat acacttcgtg aatgtttgtc cggccagttt
     8401 ggttttggcg ttgaacgcga tgccgtgttt ggctttttat cgctgatctt ctggctgcta
     8461 atctttgtgg tttccattaa atatctcacc ttcgtgatgc gggcagataa cgccggtgag
     8521 ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata cgtcggcgcg aaccacatca
     8581 atgctggtga ttatggggct aatcggcggc agctttttct atggtgaagt cgtcataaca
     8641 cccgctattt cggtgatgtc agccattgaa ggtctggaaa tcgtcgcccc gcagctggat
     8701 acctggatag ttcccctctc aattatcgtt ctcactttat tatttatgat tcaaaaacat
     8761 ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc tgacctggtt tttgattctg
     8821 gcaggactgg ggttacgtag cattattgct aacccggaag tgctgcatgc gctgaatcca
     8881 atgtgggcgg tgcatttctt ccttgaatac aaaacggttt cttttattgc attaggggca
     8941 gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg atatggggca ctttggtaag
     9001 ttccctattc gcctggcgtg gttcaccgtc gtattacctt ccttgaccct taattacttc
     9061 ggtcagggag cgctgttgtt aaagaacccg gaagcgatta agaacccgtt cttcctgttg
     9121 gcaccggact gggcgctgat cccgctgctg atcatcgccg cactggcgac ggtaattgcc
     9181 tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc aggcggtacg tctgggatat
     9241 ttgtcgccga tgcgcattat tcacacctcc gaaatggagt cagggcaaat ctatattccg
     9301 tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga ttgtcagctt tgagcactcc
     9361 agcaacctgg cggcggcgta cgggattgcg gtgaccggaa ccatggtgct gacgtctatt
     9421 ctctcgacta ccgtggcacg tcagaactgg cactggaata agtattttgt tgcgctgatc
     9481 ctgattgctt tcctttgtgt cgatattcca ttgttcaccg ctaacctcga taaactgctc
     9541 tccggcggct ggttgccatt gagcctcggt actgtgatgt ttatcgtgat gaccacctgg
     9601 aagagcgagc gtttccgctt gctgcggcgg atgcatgaac atggtaactc tctggaagcg
     9661 atgattgctt cgctggagaa atcaccgcct gttcgcgtgc ccgggaccgc ggtgtatatg
     9721 tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata accttaaaca taacaaggta
     9781 ttgcatgagc gggtgattct gttaactctg cgtaccgaag acgcgccata tgtccataat
     9841 gtccgtcggg tacagattga acaactgtcg cccactttct ggcgcgtggt ggcaagttat
     9901 ggttggcgag aaacgccaaa cgtggaagaa gttttccacc gctgcggtct ggaaggatta
     9961 agttgccgga tgatggaaac ctccttcttt atgtcgcatg agtcgttgat cctcggcaaa
    10021 cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc tgctgcaacg taatgcgctg
    10081 cgtgcaccag atcaatttga aattccgcca aacagggtta tcgaactggg tactcaggtc
    10141 gaaatctaac gccagacgcc tcctttctcc ataagggggc gtttttgttt tcatggttaa
    10201 tcaccatgta aaacgtttcg aggttgatca catttccgta acgtcacgat ggttttccca
    10261 actcagtcag gattaaactg cgggtcagcg aaacgtttcg ctgatggaga aaaaaatgaa
    10321 aaaaggcacc gttcttaatt ctgatatttc atcggtgatc tcccgtctgg gacataccga
    10381 tacgctggtg gtgtgtgatg ctggtttacc catccccaaa agtacaacgc gtatcgatat
    10441 ggcattaacc cagggtgtac cttcttttat gcaggtgctg ggcgtcgtca caaatgaaat
    10501 gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa cataatccgc aactccacga
    10561 aacgttgctc actcaccttg agcagctgca aaaacaccag ggaaatacca ttgaaattcg
    10621 ttacaccacg catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg taattcgcag
    10681 cggagaatgt tctccgtatg cgaatatcat tctctgtgct ggcgtgacgt tctgaggccg
    10741 tcatggaagc attacttcag cttaaaggca tcgataaagc cttcccgggc gtaaaagccc
    10801 tctcgggcgc agcgttaaat gtctatccgg gccgcgtgat ggcgctggtg ggcgaaaacg
    10861 gcgcgggtaa atccaccatg atgaaagtgc ttactggtat ctatgctcgc gatgccggca
    10921 cgcttttatg gctggggaaa gaaacgacat ttaccgggcc gaaatcttcc caggaagccg
    10981 ggattgggat tatccatcag gaactgaacc tgatcccgca gttgaccatt gccgaaaaca
    11041 ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat tgactggaaa accatgtatg
    11101 ccgaagcgga taaattgctg gctaaactta acctgcgctt taaaagcgac aagctggtgg
    11161 gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc caaagtgctg agctttgagt
    11221 cgaaagtcat cattatggat gaaccgaccg atgcgctgac cgataccgaa accgaatccc
    11281 tgttccgcgt catacgcgag ctgaaatcgc aaggccgcgg tattgtctat atctcccacc
    11341 gcatgaaaga aatcttcgag atttgcgatg acgttaccgt ttttcgtgac gggcaattta
    11401 ttgctgagcg cgaagtggca tcactgaccg aagattcgct gattgagatg atggtgggcc
    11461 gcaagctgga agatcaatat ccacacctgg acaaagcgcc gggagatatc cgcctgaaag
    11521 tcgataatct ctgcggactt ggcgttaacg atgtctcttt tactttacgc aaaggcgaaa
    11581 ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga actgatgaaa gtgctctacg
    11641 gcgcactacc gcgcaccagc ggttacgtca ccctggatgg gcatgaagtc gttacccgtt
    11701 caccgcagga tggcctggca agcggcattg tgtatatctc cgaagaccgt aaacgtgacg
    11761 gtttagtgtt gggcatgtca gtaaaagaga acatgtcgct gacagcgctg cgctacttca
    11821 gccgcgctgg cggcagtttg aagcatgccg atgaacagca ggctgtgagt gatttcattc
    11881 gtctgtttaa tgtgaaaaca ccgtcgatgg aacaggcaat tggtctgctt tccggtggca
    11941 atcagcaaaa agtggcgatt gcccgcggtc tgatgacacg ccccaaagtg ttgatccttg
    12001 atgaacctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa ctgattaacc
    12061 agttcaaagc cgatggcttg agcatcattc tggtgtcatc ggagatgcca gaagtattag
    12121 gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa tttactcgtg
    12181 agcaggccac ccaggaagtg ttaatggctg ccgctgtggg caagcttaat cgcgtgaatc
    12241 aggagtaaaa aaatgacaac ccagactgtc tctggtcgcc gttatttcac gaaagcgtgg
    12301 ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt ctcgacgtta
    12361 agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac ctcggtgaac
    12421 gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcatcga cttgtctgta
    12481 ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat tgaagtcaat
    12541 gcgctggtgg ctgtcgctgc tgctctcgcg ttaggtgccg caattggtgc ggtaaccggg
    12601 gtgattgtag cgaaaggtcg cgttcaggcg tttatcgcta cgctggttat gatgctttta
    12661 ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg ctttactgag
    12721 aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc gacgccagtc
    12781 tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac gcgtctgggg
    12841 cgttacatct acgcgctggg cggcaacgaa gcagcaacgc gtctttctgg tatcaacgtc
    12901 aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct ggccgggatc
    12961 attgaagtgg cgcgtctctc ctccgcacaa cccacggcgg ggactggcta tgagctggat
    13021 gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg tcgcattgtt
    13081 gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa tttgttaggt
    13141 gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc ggtgctggta
    13201 gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat gaaaaaactg
    13261 gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc gatggcaaaa
    13321 gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc gctgaaagat
    13381 ggcgcgcaga aagaggcgga taagcttggc tataacctgg tggtgctgga ctcccagaac
    13441 aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac caaaattctg
    13501 ctgattaacc cgaccgactc cgacgcagta ggtaatgctg tgaagatggc taaccaggcg
    13561 aacatcccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt gagccacatt
    13621 gcttctgata acgtactggg tggcaaaatc gccggtgatt acatcgcgaa gaaagcgggt
    13681 gaaggtgcca aagttatcga gctgcaaggc attgctggta catccgcagc ccgtgaacgt
    13741 ggcgaaggct tccagcaggc cgttgctgct cacaagttta atgttcttgc cagccagcca
    13801 gcagattttg atcgcactaa aggtttgaac gtaatgcaga acctgttgac cgctcatccg
    13861 gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct gcgcgcactg
    13921 caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc ggatggcgaa
    13981 aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga tcagattggc
    14041 gcgaaaggcg tcgaaaccgc agataaagtg ctgaaaggcg agaaagttca ggctaagtat
    14101 ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat gacctgatgg
    14161 tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag ggcacgcgcc
    14221 accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca ggcagcctcg
    14281 ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt cctactccag
    14341 gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc gcgaatcagg
    14401 ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtacg ggtgatgaca
    14461 gcattggtga gagcgttcgc cagcagctcg ccactgataa cattgatatt actccggtca
    14521 gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc gaaggtgaga
    14581 atgtcatcgg tattcatgcc ggcgctaatg ctgcccttac cccggcgctg gtggaagcgc
    14641 aacgtgagcg tattgccaac gcgtcagcat tattaatgca gctggaatca ccactcgaaa
    14701 gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg cttaacccgg
    14761 ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt acgccaaacg
    14821 aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat gcagcgaagg
    14881 cggcgcaggt actgcatgaa aaaggtatcc gtactgtact gattacttta ggaagtcgtg
    14941 gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg gtgcaggctg
    15001 tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg ttgctggaag
    15061 aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt gccgtaacac
    15121 gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt ttagacaggc
    15181 agaggtgacg cttggctaca atgaaagatg ttgcccgcct ggcgggcgtt tctacctcaa
    15241 cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc gccaaagttg
    15301 aagcggcgat taaagaactc aattacgcgc catcagccct ggcgcgtagc ctcaaactca
    15361 atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc tattcagaac
    15421 tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc ctttgcaata
    15481 ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa aaacgcgttg
    15541 atggcttgct gttactgtgc accgaaacgc atcaaccttc gcgtgaaatc atgcaacgtt
    15601 atccgacagt gcctactgtg atgatggact gggctccgtt cgatggcgac agcgatctta
    15661 ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc gataaaggtc
    15721 atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc ctgcggttgg
    15781 aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc tatgaagtca
    15841 ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta tcacatccgc
    15901 tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt taccaggcgt
    15961 tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat gacgatatcg
    16021 aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa gatgaactgg
    16081 gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt cagcaacaac
    16141 gattacaact tactccgatt ctgatggaac gcggttcggc ttagatttac gctgtctttt
    16201 gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg aagcaacagt
    16261 aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg tgccttccat
    16321 cccttcataa acgcgaagga cggccgcact tacagcaacg cctaaactaa tcgacagttg
    16381 ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca gatcggcaag
    16441 tgtgatggta ttcatcgcgg taaattgcgt cgacatagcc atccctaata taaacaacgg
    16501 caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag cgatcattag
    16561 cccaataatc accgtgatcc ccactaacgt atggcgatag cccagacgac gtaagacttg
    16621 ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca tacagccggc
    16681 aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga acggtacaca
    16741 accggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag tgcgggtttt
    16801 aaataaatct aatgaaatta atgggtttgg cgtgtgtcgc gcatggagaa tatagagaag
    16861 cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga caatcctttc
    16921 cccgaatagc tctattccgc ttgagaagag aacaaggctg aggccaaaca gcaaaaagcc
    16981 agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc gcgcgtaaag
    17041 aaggcccgca atacctatgg ggatattgat taaaaatatc cagtgccagg ttgcccaggt
    17101 gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg gcatggcgac
    17161 aaaattcaat actggaagaa gttcattacg aggataagcg cgcagtaagg ccagccgagc
    17221 aacaggcatc atcattgcgc cgcctatccc ctgaataacc cggaagacaa ccagctgtgg
    17281 tagcgaatta gaaagtgcgc aggccagaga acccaatgtg aacagactca cggcaagggt
    17341 aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa taagcatcgc
    17401 caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac gattaaggct
    17461 atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct gcatgaagaa
    17521 ggccatcgcc gcgatccacg gcaaacccgc catactgcgc ttctttttat cgctcattca
    17581 atgtcctgtt atcgggttgt cacttatcag gtgagcgtag cagcgcctga caagctttaa
    17641 atgccgcgtc gccatcgctt tggacaatcg catcgacaat cgcctggtgc agatccagct
    17701 ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa tggaatagcg
    17761 aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca tgccaggcca
    17821 tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc atttcggcca
    17881 ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgcagcc agtaggcaag
    17941 cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga tgaaagttct
    18001 cttctgtcat ccaccaggta agcaattcct gatcaagaaa attccagttt gattgtggca
    18061 tgacccgggt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt aacgttttga
    18121 ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca atctcaccag
    18181 gcaaaatggt gccgggttca tattcacctt ttaagatgcg ttgcgccagc ttctcagcca
    18241 gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc attactaatc
    18301 ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta cggttgcaaa
    18361 aacggcaaat tgcttgtttt atggcacatt aacggggctt ttgctgaaaa aatgcgcggt
    18421 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    18481 actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaattccg
    18541 acagagaaag caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgcacacccc
    18601 gcgccgctga ga
//
LOCUS       DAZFWP020000097        18374 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFWP020000097 DAZFWP020000000
VERSION     DAZFWP020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.097"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..886
                     /locus_tag="ACHK0Z_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             <1..886
                     /locus_tag="ACHK0Z_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HGU4503196.1"
                     /translation="RTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKAS
                     DKDKERVTKNINIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTD
                     RSIIRAMMEGGTAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVS
                     DTPLDDKEKGFISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQ
                     ARAMVATGNYDEAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLS
                     ARMLSRYQNNYHFGGSTL"
     gene            883..3704
                     /gene="traG"
                     /locus_tag="ACHK0Z_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /pseudo
     CDS             883..3704
                     /gene="traG"
                     /locus_tag="ACHK0Z_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490585.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
     gene            3719..4222
                     /locus_tag="ACHK0Z_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             3719..4222
                     /locus_tag="ACHK0Z_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HGU4503197.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            4254..4985
                     /gene="traT"
                     /locus_tag="ACHK0Z_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             4254..4985
                     /gene="traT"
                     /locus_tag="ACHK0Z_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGU4503198.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            5237..7390
                     /gene="traD"
                     /locus_tag="ACHK0Z_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             5237..7390
                     /gene="traD"
                     /locus_tag="ACHK0Z_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGU4503199.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDLSGEFVKSYYDPSIDKILNPLDAHCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            7390..12660
                     /gene="traI"
                     /locus_tag="ACHK0Z_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             7390..12660
                     /gene="traI"
                     /locus_tag="ACHK0Z_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGU4503200.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMTE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLNELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKISGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFADLGREINTQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVVGLGPTHRAV
                     GEIRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPGSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREV
                     VRENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            12680..13426
                     /gene="traX"
                     /locus_tag="ACHK0Z_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             12680..13426
                     /gene="traX"
                     /locus_tag="ACHK0Z_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU4503201.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSR
                     VGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            13485..14345
                     /locus_tag="ACHK0Z_RS16110"
                     /old_locus_tag="pgaptmp_003222"
     CDS             13485..14345
                     /locus_tag="ACHK0Z_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4503202.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            14448..15008
                     /locus_tag="ACHK0Z_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             14448..15008
                     /locus_tag="ACHK0Z_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU4503203.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDETVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            15141..15353
                     /locus_tag="ACHK0Z_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             15141..15353
                     /locus_tag="ACHK0Z_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4503204.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            15598..16059
                     /locus_tag="ACHK0Z_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             15598..16059
                     /locus_tag="ACHK0Z_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGU4503205.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            16105..16314
                     /gene="hha"
                     /locus_tag="ACHK0Z_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             16105..16314
                     /gene="hha"
                     /locus_tag="ACHK0Z_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU4503206.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            16352..16504
                     /locus_tag="ACHK0Z_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /pseudo
     CDS             16352..16504
                     /locus_tag="ACHK0Z_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16505..16911)
                     /locus_tag="ACHK0Z_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /pseudo
     CDS             complement(16505..16911)
                     /locus_tag="ACHK0Z_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17033..17470)
                     /locus_tag="ACHK0Z_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             complement(17033..17470)
                     /locus_tag="ACHK0Z_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503207.1"
                     /translation="MYFIGYHGTSEASAQNILSVGVQERFLPPTGQIGRGFYIAKMNG
                     TLPQWGAENATEFARRNQPYFTRMVSLLTGASNNPWLDKAAQRTILKIYTVRPLLHCK
                     WNIMNPGDLEFLKATLYDNGGPGLDLKRDCKWLYHQRNSSTCG"
     gene            complement(17719..17946)
                     /locus_tag="ACHK0Z_RS16150"
                     /old_locus_tag="pgaptmp_003230"
     CDS             complement(17719..17946)
                     /locus_tag="ACHK0Z_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503208.1"
                     /translation="MSQRQRHSVIIHGDLHMGNILFDEKNNRFWPIDFSNSYEHYYSI
                     DKDSKLFVNKMADREFCSILKTLRENAISNI"
     gene            18122..>18374
                     /locus_tag="ACHK0Z_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             18122..>18374
                     /locus_tag="ACHK0Z_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503209.1"
                     /translation="MRQVLNINVGLGSDIPAGHSPSIFEACLHAITASKALNDGGNSQ
                     LSSEVWGYSGASVSFREAFWLATGGDGKILDLPIGKLRKD"
BASE COUNT         4586 a         4233 c         5323 g         4232 t
ORIGIN      
        1 ccggacgcag gtgtcccagc agaaagtctg tcaggacatt gccggtgaga gcaatatttt
       61 tgctgactgg gcggcttccc ggcagggatg taccgttggc gggaaatctg acagtgtcag
      121 ggataaagcc agcgacaagg ataaggagcg ggtgaccaaa aacatcaaca tcatgtggaa
      181 tgcgctttcc aaaaacagaa tgtttgacgg caacaaagag ctgaaagagt ttgtgatgac
      241 gctgaccggc tcactggtgt ttggtcctaa cggcgaaatc acacccttgt cagccagaac
      301 cactgaccgc tcaattatcc gggccatgat ggaaggcggc acagcaaaaa tttatcactg
      361 caacgattct gataaatgcc tgaaagtggt ggcagacaca ccggtgacca tcagccggga
      421 taatgcactg aagtctcaga ttactaaact tctggccagc attcagaaca aggcggtcag
      481 tgacacgcct ctggatgaca aggaaaaagg ctttatttcc agtaccacca tccccgtctt
      541 caaatacctg gttgacccgc agatgctcgg tgtttccaac agtatgattt accagctgac
      601 ggactatatc ggttacgaca tcctgctgca gtacattcag gagctgatac agcaggcccg
      661 ggcgatggtg gccacaggaa attatgacga agcagttatc gaacatatta acgacaacat
      721 gaatgatgcc acccggcaga ttgcggcgtt tcagtcacag gtgcaggtac agcaggatgc
      781 gctgctggtt gtcgatcgtc agatgagcta catgcgtcag cagctttccg cccgcatgct
      841 cagtcgttac cagaacaact atcacttcgg agggagcacg ctgtgaatga agtttatgtg
      901 attgccggtg gtgagtggtt gcggaataac ctgaacgcca ttgccgcctt tatgggaacc
      961 aggacgtggg attccattga aaaaattgcg ctcacattgt ctgttctcgc ggtggccgtg
     1021 atgtgggtac agcggcacaa cgtgatggat ttgctgggct gggtggccgt gtttgtgctt
     1081 atcagcctgc tggttaatgt ccgcacatcg gtgcagatta ttgataacag tgacctggtc
     1141 aaagttcacc gggtggataa tgtaccggtc ggactggcga tgccactttc actgacgacc
     1201 cgtatcgggc atgcaatggt ggccagttac gagatgatct tcacgcaacc ggacagtgtc
     1261 acctatagca aaacggggat gctgtttggc gcagaactgg tatcaaaaag caccgacttt
     1321 ttgtcccgca atccggaaat cgccaatctt ttccaggatt atgtgcagaa ctgcgtgatg
     1381 ggggatattt acctgaacac aaatacacgc tggaggagct tatggcctcc gctgatccct
     1441 acacgctgat tttttcccgc cccagtccgc tacggggcgt ttatgacagc aataataatt
     1501 tcgtgacctg taaggacgcg tcggtttcgc tgaaagacaa gctgaatctg gatacacaga
     1561 gtggcggaaa aacctggcat tattatgccc agcagctatt tggtggacgc cctgatccga
     1621 acctgttgtt cagtacgctg attggcgaca gttacagtta cttctatggc tcaagtaaat
     1681 cagccagcca gatcatccgc cagaatgtca ccatcaatgc gctgaaggag gggatcacca
     1741 gttatgccgc gcgtaatggt gattcagcca gcctggtgaa tctggccacc acgtcatcga
     1801 tggagaagca acgtctggca catgtctcca tcggtcatgt ggcgatgcgg acactgccca
     1861 tgacgcagac catcctgacg gggatcgcca tcggtatttt cccgcttctg gtactggcgg
     1921 ctgtcttcaa caaactgacg ctgtccgtcc tgaaaggcta tgtgtttgcc ctgatgtggt
     1981 tgcagtcatg gccaatgttg tacgccattc tgaacagcgc catgacattt tatgcgaagc
     2041 agaatggtgc accggtggtg ctctcggaaa tttcacagat acagctgaaa tactccgatc
     2101 tggcttccac tgccgggtat ctttccatga tgatcccgcc attatcctgg atgatggtca
     2161 ggggactcgg ggccggattt tccagtgtgt acagccattt cgcttcctct gctatcagcc
     2221 cgactgccag tgcggcaggt agtgtggttg acggtaatta ctcctacggc aacatgcaga
     2281 cggaaaacgt gaacggattc agctggagca ccaacagcac cacgtcgttt ggtcagatga
     2341 tgtaccagac cggcagtggc gcaaccgcta cacagacccg tgacggtaat atggtgatgg
     2401 atgcgagcgg ggcgatgtcc cgtttaccgg tcggtatcaa tgcaacgcgt cagattgcgg
     2461 cggcacaaca ggaaatggcc cgggaggcgt cgaacagagc agaaagcgcc ctgcatgggt
     2521 tcagcagcag tattgccagt gcctggaact cgctcagtca gtttggttct aaccggggga
     2581 gcagtgattc tgtcaccggt ggtgctgaca gcacgatgag cgcacaggac tccatgatgg
     2641 ccagccgtat gcgcagcgca gtggaaagct atgcgaaggc gcataatatc agtaatgagc
     2701 aggcgacaag agaactggca tcaagaagta cgagaggttc tgcggggatg tatggcgatg
     2761 ctcatgctga atggggggtt aaacctaaaa ttcttggtgt tggtggtggc gtaggtgtta
     2821 gaggtggtgg tcgggcaagt attgactgga gtgatgagga tgcgcatcag gccagcagtg
     2881 gttcacgggc cagccatgat gctcgccatg atatcgatgc cagggctaca caagacttta
     2941 aagaggccag cgattacttt accagtcgca aggtcagtga atccggcagc catacggaca
     3001 ataatgccga ttcccgtgtg gaccagctgt ctgcagccct gaactcagca aaacagagtt
     3061 acgaccagta cacgacgaat atgacccgca gccatgaata tgctgaaatg gcatcccgta
     3121 ctgaaagcat gagtgggcag atgagtgaag acctgtcgca acagtttgca cagtatgtga
     3181 tgaaacacgc gccgcaggat gccgaagcta ttctgacaaa caccagttca ccggagattg
     3241 cagaacgccg tcgggctatg gcgtggtctt ttgttcagga acaggtgcag cctggtgttg
     3301 ataatgcctg gcgtgaatcc cgtggggaca tcggtaaagg aatggagagc gtaccttcgg
     3361 ggggtggcag ccaggatatt attgctgatc atcaggggca tcaggccatt attgagcaaa
     3421 gaacgcagga cagtaatatc cgtaatgatg taaaacatca ggttgataat atggtcacag
     3481 aataccgagg caatatcgga gatactcaga gcagcatacg tggcgaagaa aatattgtag
     3541 gaagacagta ttctgaacta caaaatcacc atataacata ggcattatct cagaataata
     3601 aatataatga agaaaaatcg ggtcaggaaa gaatgcccgg ggctgacagt ccacaggaac
     3661 tgatgaaaag agcaaaggag tatcaggata agcacaagca ataatattgt atggggagat
     3721 gataatgaaa aatttagctc atattgctct ggtgacggtg atacagttca ttgcctgtta
     3781 tctggctgat tgggggagtg ccgaaacagt ttttatcctg tgctttattg tactatggca
     3841 gggattgttt atatggttat tttctcaaat acgaaaaaaa cggcatgtgt cggatgagtt
     3901 taaattcagt aaaggcgttt ggtatatact gatgccagta tcttccttac tctctccgtt
     3961 attaagcctg atggttttta tcattggtac actatacgaa ttaaggcgaa ttagtggttg
     4021 tgtaagtgta agagaatgga tgcaatctca ggtaaatgaa caaactaatg aagatttgca
     4081 tctggatttt gatgatgtga attttgatta tgtggatttt tatcgtacta atccggcaac
     4141 aggattagct atgcatggag gatttgattc agcaggaaat acttttggga cttgttggca
     4201 ggactatgat gacaggcgat aagaaaactg tggtttgcaa taaaggtaat ttgatgaaaa
     4261 caaaaaaatt gatgatggtt gcactggtca gttccactct ggccctttca gggtgtggtg
     4321 cgatgagcac agcaatcaag aagcgtaacc ttgaggtgaa gactcagatg agtgagacca
     4381 tctggcttga acccgccagc gaacgcacgg tatttctgca gatcaaaaac acgtctgata
     4441 aagacatgag tgggctgcag ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg
     4501 tggtgacttc tccggataaa gcctactact ggattcaggc gaatgtgctg aaggccgata
     4561 agatggatct gcgggagtct cagggatggc tgaaccgtgg ttatgagggc gcagcagttg
     4621 gtgcagcgtt aggtgccggt atcaccggat ataattccag ttctgccggt gccacactcg
     4681 gtgtgggcct tgctgccggt ctggtgggta tggctgcaga tgcgatggtg gaagatgtga
     4741 actataccat gatcacggat gtgcagattg cagagcgtac taaggcaacg gtgacaacgg
     4801 ataatgttgc cgccctgcgt cagggcacat caggtgcgaa aattcagacc agtactgaaa
     4861 caggtaacca gcataaatac cagacccgtg tggtctcaaa tgcgaacaag gttaacctga
     4921 aatttgaaga ggcgaagcct gttctcgaag accagctggc caaatcaatc gcaaatattc
     4981 tctgattatc ttccggaggc tggtctgacc ggcctcctga ctgacgcctc gctttgctcg
     5041 ttgtccaacc ccggaaagaa ataaaataaa ttttatttct ccccggcgtt tccgaaataa
     5101 atatgaaaac actgagcggt tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga
     5161 agccggaatt taacggttat taatcttttc atcattaata atcagtccga ctgacttttt
     5221 tcttcggaat atcatcatga gttttaacgc aaaggatatg acccagggcg gtcagattgc
     5281 gtccatgcgt atccgcatgt tcagccagat cgccaatatc atgctttact gcctgtttat
     5341 ttttttctgg atactcgttg gtctggtttt atgggtaaaa ataagctggc agacgtttgt
     5401 gaacggctgt atttactggt ggtgtaccac gctggaaggc atgcgggatt taatcaagtc
     5461 ccagccggta tatgagatcc agtattacgg caaaaccttc cggatgaacg ctgctcaggt
     5521 actgcatgat aaatatatga tctggtgcgg agagcagcta tggtccgcat tcgttctggc
     5581 tacagttgtg gcactggtta tttgcctgat taccttcttt gttgtctcct ggattctggg
     5641 gcgtcagggt aaacaacaga gcgaaaatga agtcacaggt ggtcgtcagc tgacggagaa
     5701 cccgaaagac gttgcccgga tgctgaaaaa agacggcaag gactccgata tccggattgg
     5761 cgacctgccg attatccggg attccgaaat ccagaacttc tgtctgcacg gcacggtcgg
     5821 ggccggtaag tcggaagtta tccgtcgtct ggccaactac gcccgtcagc gtggagatat
     5881 ggtggtgatt tatgaccttt caggggaatt tgttaaaagt tactatgatc cctccatcga
     5941 taaaatcctg aatccgctgg atgcccactg tgccgcctgg gatttatgga aggagtgtct
     6001 gacacagccg gattttgata atacggcaaa taccctgatc ccgatgggca caaaagagga
     6061 cccgttctgg cagggttcag gacgtaccat ttttgcggaa gcggcgtacc tgatgcgtaa
     6121 tgaccccaac cgcagctaca gcaaactggt ggacacactg ctttccatca aaatcgaaaa
     6181 actgcgtacc ttcctgcgta attcaccggc ggccaacctg gtggaagaga aaattgagaa
     6241 aacggcgatt tccatccgtg ctgtgctgac caactacgtg aaagccatcc gttacctgca
     6301 ggggattgag cataacggtg agtcttttac catccgtgac tggatgcggg gtgtccggga
     6361 agatcagaaa aacggctggc tgtttatctc gtcgaatgcc gacacccatg cctccctgaa
     6421 gccggtgatc tccatgtggc tgtccattgc cattcgtggt ctgctggcaa tgggagaaaa
     6481 ccgtaaccgt cgtgtgtggt ttttctgtga tgagctaccc acgttacaca aactgccgga
     6541 cctggtggag atcctgccgg aagcccgtaa gttcggtggc tgttatgtgt ttggtatcca
     6601 gtcctatgcc cagctggaag atatctacgg tgagaaagcg gctgccacgc tgtttgacgt
     6661 catgaacacc cgtgcctttt tccgttctcc cagccataag attgcagagt tcgctgccgg
     6721 tgagattggt gagaaagagc acctgaaagc cagcgagcag tactcctacg gtgctgatcc
     6781 ggtacgtgac ggggtatcga ccggtaagga tatggagcgc cagacgctgg tcagttattc
     6841 cgacattcag tctctgccgg atttgacctg ttatgtcacc ctgcccggac cgtatccggc
     6901 agtaaaactc tcactgaaat atcaggcacg tccgaaggtt gcccctgagt ttattccgcg
     6961 tgacatcaac ccggaaatgg agaaccgcct gagcgccgta cttgccgcaa gggaagcaga
     7021 aggtcgtcag atggccagcc tcttcgaacc ggatgtgccg gaagttgttt ccggagaaga
     7081 cgtgactcag gctgaacagc cgcaacagcc ggtgtctcct gccatcaacg ataagaagtc
     7141 agattcaggt gtgaatgttc cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga
     7201 agaagagatg gaacagcaac tgccacccgg gatcagtgaa tccggtgaag tggtggatat
     7261 ggccgcttat gaggcatggc aacaggaaaa tcatccggac atccagcagc agatgcagcg
     7321 tcgtgaagag gtgaacatta atgtgcaccg ggagcgcggg gaggatgttg agccgggaga
     7381 tgatttctga tgatgagtat cgcgcaggtc agatcggccg gaagtgccgg taactattat
     7441 accgacaagg ataattacta tgtgctgggc agcatgggag aacgctgggc cggcaggggg
     7501 gctgaacagc tgggactgca gggcagtgtc gataaggatg tttttacccg tcttctggag
     7561 ggaaggctgc cggacggagc ggatctaagc cgcatgcagg atggcagtaa caggcatcgt
     7621 cccggctacg acctgacctt ctccgctccc aaaagtgtct ccatgatggc catgttaggt
     7681 ggcgataagc gcctgattga tgcacataac caggccgtgg actttgctgt tcgtcaggtg
     7741 gaggcgctgg cctccacacg ggtgatgacg gacggacagt cagaaacggt gctgaccggt
     7801 aatctggtga tggcactgtt taaccacgac accagtcgcg atcaggaacc acagttacac
     7861 acgcatgcgg tggtggctaa tgtcacgcag cataacggcg aatggaagac actgagcagt
     7921 gacaaagtgg ggaaaacggg gttcattgag aatgtgtacg ctaatcagat tgcctttggc
     7981 aggctctacc gggaaaaact gaaagagcag gttgaggcgc tgggctatga aaccgaagtg
     8041 gtgggtaagc acggtatgtg ggagatgccg ggcgtaccgg tggaggcctt ttccggacgc
     8101 tcacaggcaa tccgggaggc cgtcggggaa gatgcctcgc tgaaatcccg ggatgtggcg
     8161 gcactggata cgcgaaaatc caaacagcac gtcgatcctg aagtcagaat gactgaatgg
     8221 atgcagacgc tgaaggaaac cgggttcgac atccgggcat atcgtgacgc ggcggatcag
     8281 cgtgcggaga cccgcacgca ggcgcccggg gctgtttcac aggaggggcc ggatgtgcag
     8341 caggcggtga cacaggccat tgccggatta agtgaacgaa aagtgcagtt tacgtacacg
     8401 gacgtgctgg ccagaacggt cggcatactg ccgccggaaa atggtgtgat tgaacgggca
     8461 cgcgccggta tcgatgaggc catcagccgt gagcagctta tccccctcga tcgtgagaag
     8521 gggctgttca cgtccgggat tcatgtgctc aatgagctgt cagtccgggc actcagtcgt
     8581 gacatcatga aacagaaccg ggtgaccgta catccggaga aaagtgtccc ccggacggct
     8641 ggttacagcg atgcggtcag tgttctggca caggaccgtc cgtcgctggc cattgtgtcc
     8701 gggcagggcg gtgcagccgg gcagcgtgag cgggtggctg aactggtcat gatggcccgg
     8761 gagcaggggc gggaggtgca gattatcgct gctgaccgtc gctcgcagat gaacctgaag
     8821 caggatgaac ggttgtccgg tgagctgata accggacgtc gtcagctgct ggaaggcatg
     8881 gccttcacgc cgggcagtac cgttatcgtt gaccagggcg aaaaactctc cctgaaagag
     8941 acgttaaccc tgctggacgg tgccgcacgt cataacgtac aggtcctgat aaccgacagc
     9001 gggcagcgaa ccggtacagg cagtgcgctg atggccatga aggatgccgg ggtgaacaca
     9061 tatcgctggc aggggggaga acagcgaccg gccaccatca tcagtgaacc ggaccgtaat
     9121 gtccgctatg cccggctggc cggagatttt gcggccagcg tgaaagccgg agaagagagc
     9181 gtggcacagg tcagcggagt acgggaacag gccatactga cacaggccat tcgcagtgag
     9241 ctgaaaacac agggcgtgct cggacaccag gaggtgacca tgacggcact gtcaccggtc
     9301 tggctggaca gccggagccg ttatctgcgg gatatgtacc gtccggggat ggtgatggag
     9361 cagtggaacc cggagacacg cagtcatgac cgctatgtta tcgaccgggt gacggcgcag
     9421 agtcacagcc tgaccctgcg ggatgcgcag ggtgaaacgc aggtggtgcg tatttcctcc
     9481 ctggacagca gctggtcgct gttccggccg gaaaaaatgc cggtggcaga cggcgagcga
     9541 ctgagggtga cagggaaaat ttccggactc cgcgtctccg gcggtgaccg cctgcaggtg
     9601 gcatccgtca gtgaagatgc gatgacggtt gttgtaccgg ggcgggctga accggcctcc
     9661 ctgcctgtga gcgattcacc attcacggca ctgaagctgg agaacggctg ggtggaaacg
     9721 cccgggcatt ccgtcagcga cagtgcgaag gtttttgcct ccgtcacaca gatggcgatg
     9781 gacaacgcga ccctgaacgg tctggcccgc agtggtcgtg atgtccggct gtattcctca
     9841 ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc cctcctttac ggtggtttct
     9901 gagcagataa aggcgcgtgc cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa
     9961 gccgggcttc acacgccggc acagcaggcc attcatcttg cccttccggt ggtggaaagt
    10021 aaaaacctgg ccttcagcat ggtggacctg ctgacagagg caaagtcgtt tgctgcagaa
    10081 ggaaccagtt ttgctgacct gggacgggaa atcaatacgc agataaaacg cggtgattta
    10141 ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc tggtttcccg tgcgtcgtat
    10201 gaggcagaaa agagcattct tcgccatatt ctcgaaggta aggaggcggt cacgccgctg
    10261 atggagagag tacctggcga actcatggag aaactgacat caggacagcg tgccgccacc
    10321 cgcatgatac tggaaacgtc cgaccgtttc acggtggtgc agggctatgc cggtgtgggt
    10381 aagaccacac agttccgggc ggtgatgtca gccgtgaacc tgctgccgga gagtgagcgt
    10441 ccccgtgtcg ttgggctggg tcccacacac cgtgcggtcg gggagatacg cagcgccggc
    10501 gtggatgcgc agacactggc gtcctttctg catgacacgc agctgcagca gcgcagcgga
    10561 gaaacgccgg atttcagcaa cacgctgttc ctgctcgatg agagctcaat ggtgggcaat
    10621 accgacatgg cacgggcata cgccctgatt gcggccggtg gcggtcgtgc cgtggccagt
    10681 ggtgacacgg accagctgca ggccatcgcg cccggtcagc ctttccgtct ccagcagacg
    10741 cgcagtgctg ccgatgtggc tatcatgaaa gagattgtgc gtcagacgcc ggaactgcgg
    10801 gaggcggtat acagcctgat taaccgggat gtggaaaggg cattgtccgg gcttgagagt
    10861 gtgaaaccgt ctcaggtgcc tcgtcaggag ggcgcatggg caccggagca ctctgtgacg
    10921 gagttcagtc acagccagga agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa
    10981 ggcgaggctt tcccggatgt ccccatgaca ctgtatgaag ccattgtccg cgactatacc
    11041 ggcaggacac cggaagcacg ggagcagacg ctgattgtca cacacctgaa tgaggaccgg
    11101 cgcgtactga acagcatgat tcatgatgca cgggaaaagg ccggtgagct ggggaaagag
    11161 caggtcatgg tgcctgtcct gaacacagcg aatatacgtg acggagaact gcgtcgtctc
    11221 tccacctggg agaataaccc ggatgccctt gccctggtgg atagtgtgta tcaccggatt
    11281 gccggtatca gcaaggatga cgggctgata accctggagg atgcggaagg taacacgcgg
    11341 ctgatttcgc cccgggaggc ggtggctgaa ggcgtcacac tgtacacccc ggacaccatc
    11401 cgggtgggaa ccggtgaccg gatgcgcttc acgaagagtg accgggagcg cggttatgtg
    11461 gccaacagcg tctggacggt gacagcggtt tccggtgaca gtgtcacgct gtcggacgga
    11521 cagcagaccc gggtgattcg ccccggtcag gagcgggcag agcaacatat tgacctggcc
    11581 tatgccatca ccgcccacgg tgcgcagggg gcaagtgaaa cctttgccat tgcgcttgaa
    11641 ggcacggaag gcgggcggaa acagatggcc ggctttgagt cggcctacgt ggccctgtcg
    11701 cgtatgaagc agcatgtgca ggtgtacacc gataaccgtc agggctggac ggatgccatc
    11761 aacaatgccg tacagaaagg aactgcccac gatgtgctgg agccggggtc tgaccgggag
    11821 gtcatgaatg cagagcggct gttcagtacg gcgcgggagc tgcgggacgt ggtggccggc
    11881 cgtgccgttc tccgtcaggc agggctggcc gggggagaca gtcctgcacg gtttattgca
    11941 cccgggcgca aatatccgca gccgtatgtg gcactgccgg cttttgaccg taacggcaag
    12001 tcagctggta tctggctgaa cccgctgacc acggatgacg gaaacgggct gcggggcttc
    12061 agtggtgaag gccgggtgaa aggcagcggg gatgcgcagt tcgtggccct gcagggcagc
    12121 cgtaacggag aaagcctgct ggctgataat atgcaggatg gtgtccggat tgcccgtgat
    12181 aatcctgaca gcggtgtggt ggtgagaatt gccggtgaag gtcgtccgtg gaatcccggt
    12241 gccatcaccg gtggtcgcgt gtggggggat attccggata acagcgttca gccgggtgcc
    12301 ggaaacggtg aaccggtcac ggcagaggta ctggcacagc ggcaggctga agaggccatc
    12361 cgccgtgaaa cggaacgccg cgcagatgaa attgtccgta aaatggcaga gaacaaacct
    12421 gacctgccgg acggcagaac agagcaggct gtcagggaga ttgccgggca ggagcgtgaa
    12481 cgggctgtca cttctgaacg ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa
    12541 cgggagcggg aggcggtccg tgaggttgtc cgggaaaacc tgctgcagga gcgactgcag
    12601 cagatggagc gggatatggt tcgtgacctg cagaaagaga aaaccctggg cggagactga
    12661 tacaggaaga taaacgctga tgacaacgga taacacgaac acgacacgta acgattcact
    12721 ggctgcccgg accgatacct ggttgcagtc gttgctggtc tggtcacccg gacagcggga
    12781 tatcatcaaa acggtggcac tggtgctgat ggtactggac catgcaaacc gcattctgca
    12841 tctggaccag acgtggatgt ttctggccgg gcgtggagcc tttccgttgt ttgctctggt
    12901 gtgggggctg aatctgtccc gccatgcgca tatcaggcag tcggccatta accggctgtg
    12961 gggatgggga attattgccc agttcgcgta ttatctggcc ggctttccgt ggtatgaggg
    13021 gaatatcctg tttgcctttg cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag
    13081 tgggtggcgt acagcagcgg ccattctcct gatggcactg tgggggcctt tgtccggcac
    13141 cagttacggc attgccgggc tgctgatgct ggcagtcagt caccggctgt accgggcgga
    13201 agacagaacg gaacgtctgg tgctggttgc atgcctgctg gccgtgattc cggcacttaa
    13261 ccttgcctcc agtgatgcgg cggctgtagc tggtcttgtg atgacggtgc tgaccgttgg
    13321 tctggtgtcg cgtgtaggaa aatcattacc ccgtttctgg cccggagatt ttttcccgac
    13381 gttctatggc tgtcatctgg ctgtgctggg cattctggcg ttgtgactgg tatgatactg
    13441 acgctgattc tgcttattaa taaacaacat aacacaggat atttatgaaa attacggatc
    13501 acaaactctc tgaaggtatt gccctgacat ttcgtgttcc tgaaggaaat ataaaacatc
    13561 ctttgattat cttatgccat gggttttgcg gtatcaggaa tgttctgctc ccttgctttg
    13621 cgaatgcctt cacagaagca ggattcgcaa cgattacttt tgattatcga ggattcggtg
    13681 agagtgatgg agaacgaggg cgtctggttc ctgcaatgca gaccgaagat attatttcag
    13741 ttatcaattg ggcagaaaag caggaatgta ttgataatca gcgtattggg ttatggggaa
    13801 catcgctggg aggggggcat gtcttcagtg cagctgcgca ggaccaacgc gtaaaatgta
    13861 ttgtcagcca gctggctttt gctgacggtg atgttctggt taccggcgag atgaatgaat
    13921 cggagagagc gtcttttcta tccacgctga ataaaatggc agagaagaag aaaaacaccg
    13981 ggaaagagat gtttgtgggg gttacacggg tactaagtga tgatgaatcg aaggtattct
    14041 tcgagaaaat caaagcccgg catcccgaaa tggatataaa aatacctttc cttacggtca
    14101 tggaaacgtt gcaatataaa ccggcagaga gtgcagccag cgtccagtgc ccagttttgg
    14161 ttgttattgc cggccaggat agcgttaatc caccggaaca gggaagagca ttatatgatg
    14221 ctgttgcttc tggtactaag gaattatacg aggaagctga tgcatgccat tatgatattt
    14281 atgagggcgc gttttttgaa cgtgtagtag ctgtgcagac acaatggttt aaacaatatc
    14341 tttaattgtc agtatttata cgttaaaggt cgttgaaatt aacttcctgt tacgatgagt
    14401 tgtttcaacg gcctgcaggg catattttca cgaaggtcag agtaagcatg gcagagcaaa
    14461 aacgaccggt actgacactg aagcggaaaa cggaaggaga gacgcctgtc cgcagccgga
    14521 aaaccatcat caatgtcacc acgccaccaa aatggaaggt gaaaaagcag aagctggccg
    14581 agaaagcagc ccgggaagcg gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
    14641 ccatttatct gaacctgccc acgctggatg agaccgtgaa caccctgaaa ccctggtggc
    14701 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
    14761 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaagctgcgc agggcgctga
    14821 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
    14881 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcggcagcgc
    14941 gtctggataa aatccgccgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
    15001 agaaataaaa aaagcctccc tcagagagag gaggcaggga aataaggctt aaaaggaaat
    15061 aacttccgta cccgaaagga agctaattca gtagattatt taacaccata atactgacgg
    15121 gttaaagagg agtgataaag atgaatggat tcagaaacag ttcacggaac ggtcaggtct
    15181 ggcgttacca gcgtgccgga agccgggccg ttattctgga agtcagtgga cgctggatgg
    15241 aagcggcaga agcatggcga cgggctgccg gtgttgcacc ccggacagac tggcagcagt
    15301 ttgcccgaaa aagggctgaa cactgccacc ggcgctgccg aggaaggggg taacgaaaaa
    15361 agcagccttc attactccag ggattcagaa ggctgaataa tgcatgagat ttgctttttt
    15421 taatatccgg tcgggcagta tgaccggaca gttctgagta gcacagagtt ttttccgtat
    15481 taagtcgtga ttttatattt tgtgattaat ttcacaaaat aaggtgttat tcagtgtgtg
    15541 ctgcaatatt caggatgcct gaaatacagg tgctatttat ctgatacgga gaataacatg
    15601 aggaaatata ttccactggt attatttatc ttttcatggc cggtattgtg tgcagatatt
    15661 catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga ctcacggaag
    15721 gcggtgcgga tcaggctggt aaatattgat gcgccggaaa agaaacagga ttatggccgg
    15781 tggtcgacag acatgatgaa atcactggtt gcaggaaaaa cggttaccgt cacatatttt
    15841 cagcgagatc gttatggtcg tattctcggg caggtttatg caccagacgg aatgaatgtt
    15901 aatcagttta tggtccgggc cggggccgcc tgggtatatg agcagtacaa cacagatccc
    15961 gtgctgcctg tccttcagaa cgaagccaga caacaaaaac gtgggctctg gtcagatgct
    16021 gatccggttc ccccctggat atggagacac cgtaaataaa gcgatggcaa tcttatcatt
    16081 catctgttct cagcgggaat aaaaatggaa aaaacaaaac aggaatggct gtatcaatta
    16141 cggcgatgtt catcagtcaa tacgctggaa aaaattattc ataaaaacag agacagttta
    16201 tcgaccagtg aacgtgaatc atttaattca gcagcagacc accggttagc agaattaatc
    16261 accggaaagt tatatgacag gataccaaaa gaaatatgga aatatgtaag gtaataattc
    16321 agcataatta attaccagga gcagataaac aatgaagctg attattttta ttctcattgt
    16381 tctgattatt gcagcgttac ttatccggat tatcctcaga agtgtaaatc agcattctcc
    16441 tttgctgatg caattgcatg cggcaggtat acggaccggt gatgcagagc gaatactgtc
    16501 tgccgtctgg tttttcagga cggtttttcc agtatctgta gctgctgcga tgaaccccga
    16561 acacatggca gagtgtgacc acaggataat gcgctctgag tttcccgatt atcgagaact
    16621 gttcagggag tctgacatca agagcgcggt agcctttttt aatatttcat tctccatttc
    16681 aatgcgttgt agctttttcc tcagctcacg tatttcgatt tgttctggtg ttatcggaga
    16741 ggcttttggt gttttgccct gacgctcatc acgcagttgt ttgacccatc ttgtcattgt
    16801 ggaaaggccg atatccatag ctttggcggc atctgccacc gtgtagttct ggtcaacaac
    16861 cagttgagcg gattcgcgtt taaactctgc gctgaaattt ctttttttca ttggagcacc
    16921 tgtgttgttc tgaggtgagc atatcacctc tgttcaggtg gccaaattca gtgtgccact
    16981 acatctttcg aaaaccagtt ctgcggcttt tcaaagatgc catcatctct cttcacccgc
    17041 aggtactgga gttcctctgg tggtacaacc atttgcaatc gcgcttaaga tccagacctg
    17101 ggccaccgtt gtcatagagt gtggccttca agaattctaa gtcgccgggg ttcatgatgt
    17161 tccatttaca atgcaatagc ggacggacag tgtaaatttt taaaattgtt cgctgagcag
    17221 ctttatcaag ccatggatta tttgacgcgc cggtcaataa gctgaccatg cgagtgaaat
    17281 acggctgatt tctgcgggcg aattcggttg cgttttcagc gccccactgt ggtagcgtac
    17341 cgttcatctt cgcaatgtag aaacctctgc cgatttgtcc cgttggagga aggaagcgct
    17401 cctgaactcc gacggaaaga atattctggg ctgacgcctc actcgtgcca tgataaccga
    17461 tgaaatacat atttgatctc aaattattaa gttatattag aagtttatgc taatactcag
    17521 gagatatata ctaaaagcgc tcaatgttaa taaagtgtta tcacaagcta ctgcccacac
    17581 caagattgtt ggccgttttg gtaggtggac gacctcttgg actctcaata tatgagcaac
    17641 attcactcct tctctcctga gttttagttg aaatgtgccg caagaatatc agtgaacaat
    17701 gccaacaata tcatttttct atatattgct tatggcattt tcccgcaaag tttttaatat
    17761 tgagcaaaac tctctgtctg ccatcttatt tacaaataat ttgctatctt tgtcaattga
    17821 ataataatgc tcgtaactgt ttgaaaaatc aatcggccaa aaacgattat tcttttcatc
    17881 aaataagata tttcccatat gtaagtctcc atgaatgatt acactgtgac gttgtcgttg
    17941 agagatatgg tgttaccgat agtcaggcat tatataatta tgatctgatg acacgaaaaa
    18001 ccattcttac ataccgcaat catatcagtg atgatcatat gcctcttata gactcagcat
    18061 ctgccggaat ttctcacagt cctctttcta atttatattt ttgcaaacag atatatttat
    18121 cttgcgtcag gttctgaata taaatgttgg tcttgggagt gatatcccag caggacatag
    18181 cccatcgata tttgaggcgt gtctccatgc aataacagca agtaaagccc ttaacgatgg
    18241 gggcaactct cagttatctt ctgaggtctg gggatactcc ggagcatctg tttcattcag
    18301 ggaggctttc tggcttgcaa caggcggtga tggaaaaatt ctggatttac caataggaaa
    18361 acttcggaaa gatt
//
LOCUS       DAZFWP020000098        18058 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFWP020000098 DAZFWP020000000
VERSION     DAZFWP020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.098"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..133)
                     /locus_tag="ACHK0Z_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     CDS             complement(<1..133)
                     /locus_tag="ACHK0Z_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HGU4503210.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     "
     gene            complement(320..703)
                     /gene="traM"
                     /locus_tag="ACHK0Z_RS16165"
                     /old_locus_tag="pgaptmp_003233"
     CDS             complement(320..703)
                     /gene="traM"
                     /locus_tag="ACHK0Z_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HGU4503211.1"
                     /translation="MAKVNMYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFLKNDDE"
     gene            1024..1626
                     /locus_tag="ACHK0Z_RS16170"
                     /old_locus_tag="pgaptmp_003234"
     CDS             1024..1626
                     /locus_tag="ACHK0Z_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU4503212.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTEHQNQ
                     RRLAYASDVYRIYTGIKSSKGIQIPATKKSLPEINSVQNN"
     gene            complement(1747..3084)
                     /locus_tag="ACHK0Z_RS16175"
                     /old_locus_tag="pgaptmp_003235"
     CDS             complement(1747..3084)
                     /locus_tag="ACHK0Z_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU4503213.1"
                     /translation="MPLLNDLLDFSDHPLMPPPSAQLFAGHLPVEWIQHCLTLSAHAT
                     VRRRRLPGDMVIWMVVAMAFFRNEPITDVVRRLNLSADGEAGMNLLARSAVTQARQRV
                     GAAPVEWLFRQTAQTWGSERYLKDDWHGLQLFAIDGAPFRTPDEPELREYYGSANTST
                     ERQSAYPVMRLVALMNLGSHILLNAVTAPYRRSETVPAHSMLATIPDNSITLFDKLFY
                     SADLLLTLNQQGCNRHWLLPAWKNIAAETEESYGPGDRLLKLKVSLQARKKNPALPEF
                     WYARAVTYEVNGVEKTVQTSLPADRYKAKEVAELYHSRWEIEVGFRNLKSSLLNNALV
                     LRSRKVELLEQEVWGMLLAYNLIRREATKAAEKHKKAASEISFKFAFQFIATEMIVLG
                     NTASPGTIPKRLEHLRGALEVVFITKRPRPSRPRAVKISKTRYPVKRSTAPLK"
     gene            complement(3363..4180)
                     /locus_tag="ACHK0Z_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /pseudo
     CDS             complement(3363..4180)
                     /locus_tag="ACHK0Z_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(4299..4585)
                     /locus_tag="ACHK0Z_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /pseudo
     CDS             complement(4299..4585)
                     /locus_tag="ACHK0Z_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4610..4816)
                     /locus_tag="ACHK0Z_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             complement(4610..4816)
                     /locus_tag="ACHK0Z_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4503214.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQIPAKRQNRGSRNSKDDGG"
     gene            complement(5362..5553)
                     /locus_tag="ACHK0Z_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             complement(5362..5553)
                     /locus_tag="ACHK0Z_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503215.1"
                     /translation="MNINTETREILRNYRAVINVRRRDMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(5550..5972)
                     /locus_tag="ACHK0Z_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     CDS             complement(5550..5972)
                     /locus_tag="ACHK0Z_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4503216.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(6019..6420)
                     /locus_tag="ACHK0Z_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     CDS             complement(6019..6420)
                     /locus_tag="ACHK0Z_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4503217.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLGFLYAEASVYVLAKQMMP
                     EYEGGFWHFIRLPDGDRFHLVNGANWFDRTVSADAAGIILTSLVINRQLWLYHDSGDA
                     GLTHLYRMRDAQSWSHIEVHPECNAIYAALD"
     gene            complement(6837..7613)
                     /locus_tag="ACHK0Z_RS16210"
                     /old_locus_tag="pgaptmp_003242"
     CDS             complement(6837..7613)
                     /locus_tag="ACHK0Z_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503218.1"
                     /translation="MNENTTLNALICRHARDLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTLRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCLEHILSSGLACDV
                     LRIPDEPPRQWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(7659..8145)
                     /locus_tag="ACHK0Z_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /pseudo
     CDS             complement(7659..8145)
                     /locus_tag="ACHK0Z_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(8105..8326)
                     /locus_tag="ACHK0Z_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             complement(8105..8326)
                     /locus_tag="ACHK0Z_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503219.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(8327..9010)
                     /locus_tag="ACHK0Z_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             complement(8327..9010)
                     /locus_tag="ACHK0Z_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU4503220.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(9087..9392)
                     /locus_tag="ACHK0Z_RS16230"
                     /old_locus_tag="pgaptmp_003246"
     CDS             complement(9087..9392)
                     /locus_tag="ACHK0Z_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503221.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSSVGLTRYRTR"
     gene            complement(9396..9813)
                     /locus_tag="ACHK0Z_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /pseudo
     CDS             complement(9396..9813)
                     /locus_tag="ACHK0Z_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9736..10856
                     /locus_tag="ACHK0Z_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /pseudo
     CDS             9736..10856
                     /locus_tag="ACHK0Z_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527236.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(10997..11959)
                     /locus_tag="ACHK0Z_RS16245"
                     /old_locus_tag="pgaptmp_003249"
     CDS             complement(10997..11959)
                     /locus_tag="ACHK0Z_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HGU4503222.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGEIKTFTLKSGKQAKFIKT
                     VVLSGDIESKTFVDVSVNGRDQTMLSRESVSDITRTIKLQQFFPAIGREVDGRIEVLD
                     GTRRRAACIYNNVKFEILVTKDSIDIADARQLAKDIQTAREHSLRELGKRLEVTYGAS
                     MTKEEIAQAESISPAKVTRAFQAASVPDEMISVFPIINDISLSDYQFLLKLSEKATNK
                     KMSVEEFMDRVRQKLMALPDYPTIEKSKILAVFRSEGKLLTDRPVKTVETEKLKEFSD
                     RNQFARKKTDPRKRLVVYEFSRISNEVQLEIDEAIKNILRKLPE"
     gene            complement(11956..13161)
                     /locus_tag="ACHK0Z_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             complement(11956..13161)
                     /locus_tag="ACHK0Z_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4503223.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTEYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEESGYEFNKKRVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDVHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNNLDAETLREAVIRPTMIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGFKPSEVLRKTIIDRIADDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTSKRDHETSYSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            13532..13705
                     /locus_tag="ACHK0Z_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             13532..13705
                     /locus_tag="ACHK0Z_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503224.1"
                     /translation="MMEIKAQQFVTSTGRQVLTDNGQQGMGGVAEAIFANCAELDNDQ
                     LNEIIEWVRLYQR"
     gene            13879..14130
                     /locus_tag="ACHK0Z_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             13879..14130
                     /locus_tag="ACHK0Z_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4503225.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYC
                     VPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLDKYL"
     gene            14127..14414
                     /locus_tag="ACHK0Z_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             14127..14414
                     /locus_tag="ACHK0Z_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4503226.1"
                     /translation="MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKL
                     RGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGKRERSDVYNLASERMR"
     gene            14416..14821
                     /locus_tag="ACHK0Z_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /pseudo
     CDS             14416..14821
                     /locus_tag="ACHK0Z_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15467..16444
                     /locus_tag="ACHK0Z_RS16275"
                     /old_locus_tag="pgaptmp_003255"
     CDS             15467..16444
                     /locus_tag="ACHK0Z_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU4503227.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVTGDKVE
                     LPFVEFAKLCGIPSNQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(16728..17468)
                     /locus_tag="ACHK0Z_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             complement(16728..17468)
                     /locus_tag="ACHK0Z_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4503228.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(17592..17711)
                     /locus_tag="ACHK0Z_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /pseudo
     CDS             complement(17592..17711)
                     /locus_tag="ACHK0Z_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            17783..18045
                     /locus_tag="ACHK0Z_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /pseudo
     CDS             17783..18045
                     /locus_tag="ACHK0Z_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4561 a         4644 c         4427 g         4426 t
ORIGIN      
        1 tgcaaaaaat aaattttcca gtttcgtccc tgatacaggc tggatttgga taatcctgaa
       61 taactgccgt cagattttcc agcagatcta tttgacgagc atgtttttgt tgaatacgat
      121 ccatcggata cataggaacc tcctcacaaa ggattctatg gacagtcgat gcagggagtt
      181 cacgtctccc tgcatcggcg attttcaatt tcaaatttta acgtggcatt aaccacgtaa
      241 ttaatatact aattatcttt cgtactgtta cctttttaaa tcataaaaac tctgatattt
      301 gaacgaagtc aaatttcttt tattcatcat cattttttag aaaaaatcgt tccatctcag
      361 atgatacctt ctccctgata tcttcaacca tattggcata ttcaaacttt ggatttccgg
      421 agacatgagg actgagagac tcaataccca aaattttcgc tactgatgat tgtgttttta
      481 caacgcattc aagaagcaat ttattaaact cagtctgatt aaatgcagac tctttacgct
      541 ccatctgagc ctcatgtaca cgaagcccca gttcaagaag cattgaagct gttgctgaaa
      601 aactgacatc tttttccctt gccccttcct gtcgacgctt ctcaataatc gcatttattt
      661 tttcataggc atcattgctg atatacatgt tcaccttagc cattaaagat tacctttttt
      721 gaacataaaa taaagttaat ttagaaacac taaaattcat aatgcaaaca gggacgcacc
      781 gctagcagtg cccctggcgg tatcctataa agaaacacac cgcgccgcta gcagcacccc
      841 taatataaaa taatgttttt tataaaaata gtcagtacta cccctacaaa gcggtgtcgg
      901 tgcgttgttg tagccgcgcc gacaccgctt ttttaaatat cataaagaga gtaagagaaa
      961 ctaatttttc ataaaactct atttataaat aaaaatcagc aaaaacttgt ttttgcgtgg
     1021 ggtgtggtgc ttttggtggt gagaaccacc aatctgttga gcctttttgt ggagtgggtt
     1081 aaattattta cggataaagt caccagaggt ggaaaaatga aaaaatggat gttagccatc
     1141 tgcctgatgt ttataaatga gatctgccat gccactgatt gctttgatct tgcaggccgg
     1201 gattacaaaa tagatcctga tttactgaga gcaatatcat ggaaagaatc ccgttaccgg
     1261 gttaatgcca tcggtattaa tccggtaacg ggatatggca gcggactgat gcaggtagat
     1321 tcccagcatt ttaacgaact ggcccgctat ggaattaagc cggaacatct gacaacagat
     1381 ccctgcatga acatttatac cggtgcttat tatctggcaa tagcctttaa aaaatggggc
     1441 gtctcctggg aggccgttgg tgcatacaat gccggattca ggaagaccga acaccagaac
     1501 cagagacgtc ttgcctacgc atcagatgtt taccggattt ataccgggat aaaaagcagt
     1561 aaaggtatcc agattccggc cacgaagaaa tcacttccgg aaattaacag cgtgcagaac
     1621 aattaatctt acagacattg tcagataccg gttatgccgc aaaagcggca tggaaggcca
     1681 cacacaaaaa tatcatatga gtgataatac tgactggccg aagccccgcg aagcgggcaa
     1741 tgccgttcac ttaagcggag cggtgctgcg tttcaccgga taacgggttt ttgatatctt
     1801 aactgccctc ggccttgatg gtcgggggcg ttttgttatg aacacaactt ccagagcacc
     1861 ccgcagatgc tccagtcgtt tcgggatggt ccccggtgat gccgtatttc ccagcactat
     1921 catttctgtc gcgataaact ggaacgcgaa cttaaagctg atttctgacg ccgctttttt
     1981 gtgtttctca gccgcttttg ttgcctcacg ccgtatcagg ttgtaagcca gcaacatgcc
     2041 ccacacctcc tgttccagca gctcaacctt acggcttctc agcaccaggg cattgttcag
     2101 caggctgctt ttcaggtttc tgaacccgac ttcgatttcc catcgtgaat ggtaaagttc
     2161 cgctacctct tttgctttgt agcggtctgc cgggagtgat gtctgcaccg ttttctccac
     2221 accgttcacc tcataagtca ccgcccgggc gtaccagaac tctggcagtg caggattctt
     2281 tttcctcgcc tgcagcgaca ccttcagttt cagaagcctg tctccgggac cgtaactttc
     2341 ttctgtttct gccgcgatat tcttccacgc aggcagtagc cagtggcggt tacaaccctg
     2401 ctggttcagc gtcagcagca ggtctgcgct gtaaaagagc ttgtcaaaca gcgtaatgga
     2461 gttatccggg atagtggcga gcatggagtg agccggcacg gtttcgctcc gccggtaagg
     2521 cgcggtcacg gcattcagca aaatgtggct tcccagattc atcaacgcca ccagacgcat
     2581 gaccgggtag gcgctctgcc gctcagtgga tgtgttggca gagccataat attcacgcaa
     2641 ctcaggttca tcaggtgtcc tgaacggtgc gccatcaatg gcaaaaagct gcaggccgtg
     2701 ccagtcatcc ttcaggtaac gttccgagcc ccatgtctgt gcggtctggc ggaagagcca
     2761 ttccaccggg gcggccccca cgcgctgacg cgcctgggtg acagcgctgc gggccagcag
     2821 gttcatcccc gcttcgccat ccgcgctcag gttcagacgg cgaaccacat cggtaattgg
     2881 ctcattgcgg aaaaaggcca tggccaccac catccagata accatgtccc ccggtaaacg
     2941 acggcggcga acggtcgcat gcgcagaaag cgtcagacag tgctgtatcc actcgacggg
     3001 aaggtgccct gcaaacagtt gtgcagaggg cggtggcata agcggatggt cactgaaatc
     3061 gagcagatca ttgagaagtg gcataagaaa acggctccct gttgtggaag ccgttatagt
     3121 gccgcagttt aaggatcggt caaccgatcc ttaactgatc ggcattgcgc gaagcgggaa
     3181 cccatcttat atataaccgg taactgcgca gcctcctgcc agaccatttt tcgcgggcac
     3241 aatatttaca gacaaccgga aagccagttg atgaaattga ggtatatcag acgacacagc
     3301 gatgccgaag aatgataata taagtaaagc cccgaaaaat tttcggggct ttacttatat
     3361 tatcatgact tccactttct gaactgttcg gcaatcaccc ataacgcctt gttgattctt
     3421 atatcaccat caatacctgt aatggctctt gtcctggtat ttttcccgga ggcacttcgc
     3481 cccgataatc caccttttat catattctcc tgagcccgct gccaggttgt ccagagatcg
     3541 ttctgtttat tctcccgacg acggggagta attatctgtt caggcgtcac tggcgtttta
     3601 ttttcatctt catacctgac catcagtgca gctctgccaa ataaatgttg ctcgtcactg
     3661 ttaagatgaa tttctttcat cgcctccata ttatcagtga ccttatcaaa gacaccgagt
     3721 acttcatacg ctccctcgat aacctgcccg acaatatcac ctttatgtgg aacgcggatt
     3781 tcgccaaaat tatttccgca caccaggcca tttgtgcaga caaaacgaaa aattccgggg
     3841 atcatctgat aactggatga accatcatgt gaattaagca ggataatttc aggaacttcc
     3901 tgcccgttaa tatgcccttc cctgcgaaga cgtaacatat gtttactgta ttcgcgacgt
     3961 cccaaatcac gaacccgact ctgacaggca aagaatggct ggaaaccttc atcacgtaac
     4021 ttattgatga tattgttgga atatacgtat aacgttcact ccgggactca tgtttatcac
     4081 cggaaaatac cgaaggcacg aactgcatta attcatcatc cgttaaagga cgttcacggc
     4141 ggatggaatt ataccgacca aaacgggaag ctaatcgcat aacagctact cctttatgaa
     4201 agaataaatt taataaaaaa cttttccgct taatatcatc cctcccggtc actattaaca
     4261 agattgcaac aatcaggaga aatattcatc acatctggtc agcagatcgc ccggatatcg
     4321 aggccggcac gcttaatctg acgcaggaac tgtttgaagt gatcggcttc cgcaatgttg
     4381 atatggcgga actgcgtatc cacaccccgg ctgagcgcgt agctatagct gaaggccatc
     4441 ttttccgggt ggcttttgcg atattttttc agctcagtca ggctgctgaa ttccaggacg
     4501 tcccactctt ccccggtgtt tttttcttcc cggcaacaag aagcgtgtac gacgcggtgt
     4561 taacgtggat atttcgggta ctcatcctgc tttctcctct ctctggtttt cagcccccat
     4621 catcttttga gtttcgggag ccgcggtttt gacgttttgc ggggatctgc cttttgttcc
     4681 ctccggtatt caccatcacc tttttgagaa ccttcgccgc aaggggcatc actgccacgg
     4741 ccggaaacga ctgtccgggg gcagaccgca aaagtttttg cctgcttttg ggcaaaaagt
     4801 tttgcgggat gtacatttca ccctggactg gcggagacgt ctttgccccc ggcgacaggg
     4861 gcttaaacag gtgaatggca ccggacacaa caacgggcag ggacccgccg caacgacgga
     4921 gagcgcgtgc gcgaaacggc gttgcccctt acatcccggc ggccttttgc cggaatgcca
     4981 ggagcgcagg ccacggcgcg acagaggcgg tgcgaccgga acggtcgctg caagccaggg
     5041 tttcgcgtca gggatggaag cccgccaggg cggagacagc ttcgctggct ccgttcacga
     5101 cagcccgccc cgccaggggg acgcccggaa aagccataaa caggagagga acggaaagaa
     5161 gaggaagagg aaaaagaaga agaacaataa caagcacggt aaccatagaa aaataacatc
     5221 agaaaaaaac attccggaca catcacaaac aggaagggta acgggcgacg cgtcagcatg
     5281 gcccgcttcc cttccggcag gcgtcagcct gccaggggtt ttcgcgcggg ctgacaggcg
     5341 gcctccaccg cctgccctgt atcacctgtt tctgctgccc tgaaggatat agtgaccacc
     5401 gaggaaaacc gcctgctgat tcgccacaaa atcgcagatt tcatcaacaa tctgcgcggt
     5461 ggtgagcggt ttctgcccca tatcacgacg ccgcacatta atcacggccc tgtaattacg
     5521 cagaatttcg cgggtttccg tgttgatatt catgacatgt gctccccgtc cagcgcctgt
     5581 aacacaacgt ccattgcttc gcgttcttcc ctgacgaatg cgtcaccgtc gcccccgttc
     5641 tcactcccga cggcatacag gcgcttcatt tcactgcctg ccagcgccat cactttttca
     5701 agcatcactg ccgggactgt cacctgacgg ctggcacgct tttcttccat cagttcacgg
     5761 acaacgtcgt gaacaatgct gacatccgca ccatgctcaa aagcatcgtc cagccgttgc
     5821 atgacggttt ccagctcatc gtctgtcaga ctccagtccc gggctatgtg acgcacatcc
     5881 ccacgggtca gcaccacggc aaaaacgcat tcactgtccg gcacgtctgt atccagcact
     5941 tcgcggataa tttctttaac agtacagtac atgttctcgt ctcctttaca ggggcggcat
     6001 tgccgccccg tccgttaatc agtccagtgc cgcgtaaatc gcgttacatt cgggatgaac
     6061 ttcgatgtgg ctccacgact gcgcatcacg catccggtac aggtgtgtca gtcccgcatc
     6121 accgctgtcg tggtacagcc acaactggcg gttaatcaca agggaagtca gaatgatgcc
     6181 tgcggcatcg gcgctgaccg tgcggtcaaa ccagtttgca ccgttaacca gatgaaaacg
     6241 gtcaccgtcc ggcaggcgga tgaagtgcca gaagccgcct tcatattccg gcatcatttg
     6301 tttcgccagc acgtacacag acgcttcggc atacagaaaa cccaggccaa acaggtaagg
     6361 cagaaaggag agacggtcgc actcttcaac attcagagtg acaggtttcg catattgcat
     6421 gatgttttcc tccgttgttc acatattccg cgacaccggg cggtgccgtg cctccataaa
     6481 agccgggcga taaggcgggg atgcaggggc gcaagggaag ccgcagcctg acgcacacgg
     6541 ctggcgggag ttggagggtg agaccgcgaa acctgagcgg gtgaacgacc ggaaaatcac
     6601 ggcagacggg aaggagggac cgggtgaccg cagcggcgaa gcgtaccctt gcatccccgc
     6661 cagcccggct aagatggagg cacggcacag cccgcacaca acacggcggc cacataactg
     6721 aaaacccaca aggccatgcc ccgcatggcc gggtaaagcg gagccggtgg cgacttcctt
     6781 tgagctctgt ccggggaccg gcaggaaaaa ggcggggatt taccccgcca acgtcatcat
     6841 ttccggattt ttgccagggc atccgccatg ctgcggattt cggcccgggc ttcccggaca
     6901 acttcccgca gaacaccacg gtcaaaccac tggcggggcg gttcatccgg tatacgcagc
     6961 acatcgcagg ccagcccact ggacaggata tgttcaagac aatcgctgac cagatacatt
     7021 tccaccgccg aaaaacgtgc gccccggttc aggatggcat cgagcaccac gggcaggttt
     7081 tcgtcgtcct catcgagcca gcaggggtga tcgctttcct tcacgactag gcgaaagcgg
     7141 cgcgtctggc gggtcagtaa cgtctgaccg gtggtggtca gcgccgcccg gatacgcagc
     7201 gtatcttctg ccacccggca actgacactg cgtacagcat cacgcaccgc cgcaagtaca
     7261 gccctgattt cgtcttcggt cagccattca ccggcatacg gaaaagacac ctgcgttccg
     7321 tctgccggaa gtaccggcag cgactgccac tgactgccgg acagtacaag ttccgccccg
     7381 gttccggtca gtttgtgaat agcggaggct aggagcggcg gcagtacgcc gccgtgacgg
     7441 ttgataagta acgtcgccag ccggggcgca tccagcagaa cataaatgct gatccagccc
     7501 ggatacttcg ggttccgctg gtcaacatca gtctcttccg gccagccctg cgccagcagc
     7561 aggtcgcggg cgtgacgaca aatcagtgca ttcagcgttg tgttttcgtt catgattaat
     7621 cgttctccct gtatcgccgc ccggaggcgg cgataacatc agtcgttttc gtgcgtgttg
     7681 tttttcagca ggatgcgggc ctgattaacc accgcctgac ggcgggtgac gaattccggg
     7741 acggcgaggc cgttatcacg ggcggcccat tcacgcttcc acaatgcctg ttcagcggtc
     7801 tgaatcaggg acgccagcag ttcagcaggg acggtcacct ggcggttttc actcacgttg
     7861 ctgataatct ccatagccgc accggaagaa ataccggact cagtccgctg gtcttccggg
     7921 atgtcctcca gccgcgccag gacggtcctg atttcatgat cggacaggga aatatccatt
     7981 ccatcagcga ggacctgaat ctcttccggg gaccagacaa ccagcatcag cggagtgtct
     8041 cccggatact gcgctgccaa ctcccggcag agcgtttcgc atgttccgta catactctct
     8101 cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc tccatcaaga
     8161 ggcggtttgc gcgaaacagc gtctgacgca ccagacgttc cggcagggtg tcggcattaa
     8221 agctgtaccg ctgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca
     8281 cctccgccac ttcggcgcgc agtttttcgt gtccggcata gttcatttac gcagcctccg
     8341 gttcaaacca gttatcattc gcggccccct gctgcatggc ccgttgcacg gcggccagtc
     8401 gttgctgtcc ggcacggtga tactgttcaa gcagctcgat accgatatac cggcgtccgg
     8461 actgaagggc ggccacgcag gttgagccgc tgcctgcaaa cgggtccagc acaattgcgt
     8521 tcggatgtgt gaagctctca atcagtggtt gcaggctggt aacgggcttt tccgtcgggt
     8581 gatggcggtt gcctgaatat ttccagccca gcacgtcggg caatggcttc tgaggcagcg
     8641 ccggacgccc ttttgccagg atgtaagcgc attcgtggcg atagcccaca tatgcggcct
     8701 tcgatgtgta ggttttggta aataccaggt ggccaaccac gctgaatccc gcatttttcc
     8761 aggcggccat aaagcgatcg acgcggttcc agccatagaa gctcaccatc agcgcatctt
     8821 ttttcagcac tcggtacatt tcattacagg ccggttgcag ccattcatcg gttttatcgc
     8881 cggcgatggt acgcccctga cggtcacgga aaccgacgag atatggcggg tcggtgagga
     8941 tgaaatcgac agcattgccc ggaaacgtgg ccataacacg cacgcagtca ccctggataa
     9001 aacgggacat aacagcactc cttctcttac agagggcacc gggagggcgc ccctgtggcg
     9061 cccgttcccc gctgcctgtg ggctgttcac cgcgtccggt aacgggtcag gccgaccgag
     9121 gaataccgca gccgtcgtct ttttgacggc gaggatatgc cgcgaaagcg gcttgaaggg
     9181 cgttcatcag aaaagggcga cgggctggcg tacactgtca ccaacgccag ccgggagcgg
     9241 taaaagcgcc cgtcccgtgc ccggccggac gcgggacact gaccacaggc agcgttcctg
     9301 ccgcgccgcg tcagccggcg cagtaaaaag aggagcccgg cgacatcctt taccggaaaa
     9361 gaaacgggcg gttgcccgcc cgtctccgtc atacttcatc ctccataatg cgccacattg
     9421 tcgactatcc acgccggtcc ggtgacttcc ggcagctcat cgtcatctgt cggggaagac
     9481 cattcaagtt cccccccaca gcacatcaac gagctctcca cgctcataca actgccagcc
     9541 acagaaatca cagccctgtt cggcatacca gtgctccagt gtgcaactga aacggcggct
     9601 taattctgca ataacgtcgc tttctggctg acaccacggc gtgtcaaaat ccacctgaat
     9661 gaagttgtct ctctgactgc tgatattcac ctcatacccc acaggccact tcacgccata
     9721 ctgttcggta taccagtgat atgcagaagg aacaccgttc agaacaccac cgttaaagcc
     9781 gtttacttca acatccaggc ggctgggcat caaaatgggg taccgtggtt tcagctgaag
     9841 tcggtgaaat tttcggagcc ggtcatcgtc ttctccagta attacccgct atacgcatcg
     9901 atgtcgaaca gggtcatggt tcatctggag gcgctggcac cgcgcgttga gcagtactcg
     9961 attgacgaga tgtttctcga cgtgcgaggt attgacagct gcatcgattt cgaggatttc
    10021 gaccagcagc tgcgtgaaca cgtcaggaac gggactggcc tgactatcgg tgtcggaatg
    10081 gggccaacca aaacgttggc aaagtcaaca caatgggctt ccaaagagtg gccacagttt
    10141 ggtggcgtgc tggcattaac gccggacaat atccgccgtg cggaaaaact gctgtcactg
    10201 cagctggcgc acgaaccctg tatttatcag gaagaacttc aatgtggttc tggagagaaa
    10261 ggtccgggaa ctcaatggag aaagctgtat ttcactggag gaagaaccgc ccccccaaac
    10321 agcagatagt ctgcagccgg tcctttagag agcgcgtcac gacatatgag gccatgcgct
    10381 aggccatctg tcggtacgct gagcgtgcag ctgaaaagct gcggggcgag tgtcagttct
    10441 gcagacatat cgccattttt ttgaagacgt caccgttcgc agtaaacgaa ccgtattacg
    10501 ggaatgtggc cagtgaaaaa ctgctcatcc ccacgcagga tacacgggat atcattgccg
    10561 ctgccgttag aattctggac aggatatggg tggatggcca tcgatatgca aaagcgggtt
    10621 gcatactcaa cgatttcact cctaccgggg tttcgcattt gaaccttttc gatgatactc
    10681 agccccggag caacagtaac cagccgttga aagtcgtgga cagcattaac cattccggtc
    10741 tggggaaagt gtggtttgca ggccgaggca ttgcgccaga atggcagata aagcgtgaga
    10801 tgtcgtcacc ggcatacact acccgctgga acgaactacc ggtcgccaga ctttgaaaaa
    10861 aagaaattca tggtgatttc actatgaaag aattaaaaaa aatactttat attcagtaaa
    10921 ataagcatga aaaaagcgtg gtgaaaattg caaaaaaagg atgccatttg gcatcctgat
    10981 attaacccgg catatcctat tcaggcagtt ttcttagaat gttttttata gcctcatcga
    11041 tttctaactg cacctcatta gatatgcggg agaactcata aacaacaaga cgctttctgg
    11101 ggtcagtctt ctttctggca aattggttac gatcagaaaa ctctttcaac ttctcagttt
    11161 ccactgtttt tacaggccgg tctgtaagca attttccttc agacctgaag accgccagga
    11221 ttttgctttt ctcaattgtt ggatagtcag gtaaagccat cagtttttgg cgaactctat
    11281 ccataaactc ttccactgac atttttttgt tggttgcctt ttcagacagt ttcaacaaaa
    11341 attgatagtc cgacagcgaa atatcattga ttatcggaaa tacagagatc atttcatccg
    11401 gtactgatgc cgcctgaaat gcgcgtgtta cttttgccgg agagatgctt tcagcctgag
    11461 cgatctcttc ttttgtcata cttgccccat aagtaacttc aaggcgtttt cctagttctc
    11521 ggaggctatg ctctctggct gtctgtatat cttttgcaag ttgtctcgcg tcggcaatat
    11581 caatactgtc ttttgtaacc agtatttcga attttacgtt gttatagata caggcagcac
    11641 gacgacgcgt accgtctaat acttcaattc gtccatctac ttcccgacca atagccggaa
    11701 aaaattgctg aagtttaatc gtgcgagtta tgtcactaac tgactcacgc gaaagcatag
    11761 tctgatcgcg tccattaaca gatacatcaa cgaacgtttt agattctata tctccgctta
    11821 atacaaccgt tttaatgaat tttgcttgtt tccccgactt aagagtaaac gttttgatct
    11881 ctccgtcacc ttcaagcatg cgagcgaact ctgaattgct attgccgaga actcgccctc
    11941 gagctaaaac ctttttcatt tggatgctcc ccgaatgtat tcaatgcggt caaaaacggc
    12001 tttagtaaat cgttccgctt cggtacgagc ttttttcaat gcctctgcac ttcctggata
    12061 cgaaacaggg ttagcactga tgatagtgtc aaaagattca ccacatcgtt cgaacccgtc
    12121 cagtctcgga agcgatgagt caagaatatt actcgcatac acttcacgag ccaggctata
    12181 ggaagtttcg tgatcgcgct tactggtcat tttggacata aaccctatgc tcgcactcag
    12241 gcggggctca acgccttctt cttccaaacg ttctagcatt tcaggtaaac gagtcaaata
    12301 tttgagagtt gaatggaaat caacttgcgc cgggggagta ggggtgagaa gtaaatcact
    12361 cgcagccagg ccattgagaa gaaatggatc aagatgaggg ccagtatcaa taaaaacaaa
    12421 atcataatca tcagcgatac ggtcaatgat ggttttcctg agtacttcag aaggcttaaa
    12481 accaggaagg tgttcagcaa ccaaagactc ccattggctt gctacgaagc cgtcatcaat
    12541 agaagcaggg atgacatcaa cgccaggaat catagtgggt cggataaccg cttctcgcag
    12601 tgtttcagca tcgagattat ttagcattgc ttgggcggca gtttcaagga ctgtaccaat
    12661 actatgggta tggtccaaaa acatagtgct ggacgcctgc gggtcaaggt cgatcacaag
    12721 gatacgaagg tcatgacgca gcaaatccgg atgtactcgt agtgcatgcg ccagcgttac
    12781 tgtactgacg gtttttgaaa ccccaccttt caagttgacg acaaaaatta cgtaaggttc
    12841 tttatgaaca tcgcgatatt ttggtatctg tcggtgagcg taaatatcaa taacgttctg
    12901 aattgttaat gcatactgct cgacgttgcc tacccgcttt ttattaaatt catagccact
    12961 ttcttccatt tctttaacgg ccagttctac gatgcgtcgg ctcagcttcg gcaattttgc
    13021 gaccgcatta cgagtatatg tttgatgata ctctgtcaga ttgaattctt ttctttgctc
    13081 ctcaatatcc cgactcatgg cctgaagtag agcacttgct ctgagcgcta tggtaccgac
    13141 actaccgtaa tctcgtttca tagttttcct ctgagataga ttccctgccc atatattgcg
    13201 tacaaagttc aatcacattg tacacagata tgaaagaggt acaatatatt ttgcattgcc
    13261 attaaattat cgtacgttgc aagtcaccat tcacatcaaa ccgcatagtt agacattgtc
    13321 tctgagcagg cgaggggtta gacaagaccg ttgggcgcgt cagagtgtta tattaggata
    13381 cgggtaggtg acacccacct ctggcacaga acggacattc attgatgcca tgccagaatg
    13441 gacgttcagg ttattccgtc cagttctgct ggcagcacga gatctcccct ggtatagtga
    13501 tgccacggca aagtgctcaa acagggataa tatgatggaa atcaaggctc aacagtttgt
    13561 cacatcaacg gggcggcaag tccttactga caacggacaa caaggtatgg gcggcgtggc
    13621 ggaagcaatt tttgcaaact gcgcggaact ggataacgac cagcttaacg agatcatcga
    13681 gtgggttcgg ctctatcagc gctgaatgcc atcatcaggc tgcgcaagcg gcctttttta
    13741 cgccccttgt ttaattcccg cactacctgg acattcaggt gattctgtcc atctgtataa
    13801 aaaacaacca aaagacttgt taacaggtca tataaggagt atctttgaga ctggttaaat
    13861 agtcttgaaa ggtggcctat gcctaacatt attctcagtg atacaagcgc cagtgtcagc
    13921 gagctgaaga aaaatccgat ggcgacagtc agcgccggtg atggtttccc ggtcgctatc
    13981 ctgaaccgta atcagcctgc tttttactgt gtacccgcag agctgtacga aaagatgctc
    14041 gatgccctgg acgatcagga gctggttaaa ctggtagccg aacgcagcaa ccaaccgctg
    14101 catgatgtag acctagataa atacctatga ggtatcaggt aaaattcagg gaagatgcgc
    14161 tgaaagagtg gcaaaaactg gacaagacta ttcagcaaca gtttgcgaaa aagctaaaaa
    14221 agtgctgtga caatccgcat attccttccg caaaactgcg cgggataaag gactgctaca
    14281 aaataaaatt acgtgcgtca ggttttcgcc tagtctatca ggtgattgac gaacaattaa
    14341 ttatcgctgt tgtagctgtg ggtaaacgtg agcgcagtga cgtttataat cttgccagtg
    14401 aaagaatgag ataattcacg gcggattatc ggttcagccc tctcattaac cgctgatgct
    14461 gagttgatgt gtagggcttt gcgtaatgca ctggaaacac gaccgcgtga tggtcgcctg
    14521 ctgtttcatt cagatcaagg tgttcagtat aaaagcaata aatacagaaa gttactttat
    14581 ggggtaatgc aaagtatgag tcgcagaggg aattgcctgg ataattcgcc gatggaaaga
    14641 atctttcgca gccttaaaag tgaatggctt ccgaaaggtg gttatggtga ttgccatgca
    14701 gtacgcgaat aaatcagtgg ataaatgctt attacaacgt ttatcgacct catacaaaca
    14761 atggtggttt atcgccttgc cgtatgaaga aaaatggaaa caggttattc cggtgtcctg
    14821 attttatgat ccactacaca ttcctgacga acgccactgc ataaatcaca ttgcacatcc
    14881 tcagagagta cgcagtatag gaggggtata tatacattat cggtatatca ggcgacttct
    14941 gtctgtatgt tgtcggtgct cgccgcagac cggtgaccgg gcgaagcccg cgaaccggcg
    15001 aggaagcgca atgtatctgt agttacagtg agcaggtacg ccctgaagcc ctttcataaa
    15061 acggttgtgt gcgctgtcct tcgggaagat ttatccacat atccagaggc ccgatccagt
    15121 aaacagatcc atgaatgatc aataaaggat ccattaaaga tccccatacc gctgcaaacc
    15181 ttgccactca tgctccagaa ccacgatcac ataagctgtg gcatgttgat gataaactgt
    15241 aacatgctga tgataagctg tattcagtaa tacatatact gacgtaagtt aatgacataa
    15301 actatggtca gtacaccaga ctcagttgtt aaatacagac tgcaggtttt tcttcagtca
    15361 gttgacgggg gtctgacaca cgattttctg tttattcttt tactgtccac aggcaggagg
    15421 ctttctggaa aacgaaaatt cagacatcaa aaaactgttc ggcgaggtgg ataagtcctc
    15481 cggtgagctg gtgacactga caccaaacaa taacaacacc gtacaacctg tggcgctgat
    15541 gcgtctgggc gtctttgtac cgacccttaa atcactgaag aacagtaaaa aaaatacact
    15601 gtcacgtact gatgccacgg aagaactgac acgtctttcc ctggcccgtg cagagggatt
    15661 cgataaggtt gagatcaccg gcccccggct ggatatggat aacgatttca agacttgggt
    15721 ggggatcatt cattcctttg cccgccataa cgtgactggt gacaaagttg aactaccttt
    15781 tgtcgagttt gcaaaactgt gtggtatacc ttcaaaccag tcatcacgca ggctgcgtga
    15841 gcgcatcagc ccttccctga aacgcattgc cggtaccgtg atctcctttt cccgcaccga
    15901 tgagaagcac acccgggaat acatcactca tctggtacag tcagcctact acgatactga
    15961 acgggatatt gttcagttac aggctgatcc ccgccttttt gaactgtacc agtttgacag
    16021 aaaagttctt ctccagctta aggcgattaa tgccctgaag cgacgggagt ccgctcaggc
    16081 actctacaca tttatagaga gcctgccccg ggatccggca ccgatatcgc tggcgcggct
    16141 gcgtgcacgc ctcaatctga agtctccggt attttcccag aaccagacgg tcagacgggc
    16201 aatggagcaa ttgcgtgaga ttggatatct tgattacact gagatccagc ggggacggac
    16261 aaaactcttc tgtattcact accggcgtcc ccggttaaaa gcgccgaatg atgagagtaa
    16321 ggaaaatccg ttgccacctt cacctgcgga aaaagtcagt ccggagatgg cggagaagct
    16381 tgccctgctt gagaaactgg gcatcacgct ggatgacctg gaaaaactct tcaaatcccg
    16441 ctgaacataa actgtagtca gtgaagatcg ctcctttact gactacagct tatattaacc
    16501 ggggcagtga gtggtttgct cactgcagtt tatattctgt ttcctgcagt gctgtctgta
    16561 gctgagctgc catctgctgt cccttacgta agtgatcccg taacctgatg ctgaggcatt
    16621 gctcccttca taaaacatga cttactcact acagcttata tacatgctcc agcttatgtt
    16681 atgtctgttc tgctgaccac tgcttatata aggaagcgcg tatgccgtca cctcagggga
    16741 ggcagtgtac gcaggatctc cgcagcatcc cgcccgtcac ctgtgaaagg cactgccagc
    16801 gtggcagcca tatccagtgc aaacactctg gtataaacct ccatagaacg tggatccctg
    16861 tgaccagcca gtgcctgaat gactttccgg ggctggcggt gataaagcat gtgcatgata
    16921 tagctgtgcc ggaaggtgtg tggtgtaacc ggaatcgaaa agtgtactcc gtcagcttcg
    16981 gcccgtctga cagcctgctt cagccagttg cgcatggttt cgtcggtcac ggcccataat
    17041 ggttcacgac gacggggccg ggtggtgatc atccagcttt ccatctgcct gacatagctt
    17101 atatctgtca gcggaactag gcggacttca tcttttggcg ggcgtccgcg tcgcgcacgc
    17161 actttttcgg acaggatccg cacaaacggt cttgcaccat ccagatcaaa tgattccggc
    17221 gtcagcatcc gggcttcgcc aatacgcatt ccggtattcc agagggtggc gaacagcata
    17281 tggtgacgct gatccggcat atagaagaga agggcactca cttccggggc cagcaggtag
    17341 gccggtgtgg cccccgtgga agtggccatt cttctcaggg agagcgctgt tgcaaaatcc
    17401 acccccggcg caatgggtaa cagggagacg cgttctggtg aattctgcag gggaatgaca
    17461 ttgttcatga gttatttttc acgtagcaca cgcactgttt atggatacag gtattctgac
    17521 ctgaacgccc ggatccttcc acgacaataa atcgcagcgc gcggatcctt ttgtttgatt
    17581 atcacgcatt gttgtgggta aaaccaaata cgtaaaggcg cattttgccg caggtatccc
    17641 gtcttaagcc agtcatggtg cgggttgaga gcttgtttac cgtttttggg agaattgggg
    17701 taatgccagg agaggcagac atgggtaaac agagttcaga caggaaagaa gtgcaggtac
    17761 gttacgtaaa tcctaaccaa gttttgatat gcgattcgaa cgcactgcat tatctgctgt
    17821 agaaaatagc ttccggatat tcagtagttc tctgggaata tcccgatgga aaaatatgca
    17881 gcagataaat gaatatggca atagagtgca atttattcat tacgggatgc taatctacac
    17941 ataaaatatc agaaaagact attaataaga ataaacaagc ccttatatta taatagtaaa
    18001 aatatggagg tggtattttc tggatgcaag ttactcgcgt tataaaattt ctttaatt
//
LOCUS       DAZFWP020000099        18056 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFWP020000099 DAZFWP020000000
VERSION     DAZFWP020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.099"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            94..777
                     /locus_tag="ACHK0Z_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             94..777
                     /locus_tag="ACHK0Z_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4503229.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            821..5671
                     /gene="ehaG"
                     /locus_tag="ACHK0Z_RS16300"
                     /old_locus_tag="pgaptmp_003260"
     CDS             821..5671
                     /gene="ehaG"
                     /locus_tag="ACHK0Z_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU4503230.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6039..7694
                     /gene="lldP"
                     /locus_tag="ACHK0Z_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             6039..7694
                     /gene="lldP"
                     /locus_tag="ACHK0Z_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4503231.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            7694..8470
                     /gene="lldR"
                     /locus_tag="ACHK0Z_RS16310"
                     /old_locus_tag="pgaptmp_003262"
     CDS             7694..8470
                     /gene="lldR"
                     /locus_tag="ACHK0Z_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU4503232.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFSGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8467..9657
                     /gene="lldD"
                     /locus_tag="ACHK0Z_RS16315"
                     /old_locus_tag="pgaptmp_003263"
     CDS             8467..9657
                     /gene="lldD"
                     /locus_tag="ACHK0Z_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU4503233.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSINEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9843..10316
                     /gene="trmL"
                     /locus_tag="ACHK0Z_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             9843..10316
                     /gene="trmL"
                     /locus_tag="ACHK0Z_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU4503234.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10369..11190)
                     /gene="cysE"
                     /locus_tag="ACHK0Z_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             complement(10369..11190)
                     /gene="cysE"
                     /locus_tag="ACHK0Z_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4503235.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11270..12289)
                     /gene="gpsA"
                     /locus_tag="ACHK0Z_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             complement(11270..12289)
                     /gene="gpsA"
                     /locus_tag="ACHK0Z_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU4503236.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12289..12756)
                     /gene="secB"
                     /locus_tag="ACHK0Z_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             complement(12289..12756)
                     /gene="secB"
                     /locus_tag="ACHK0Z_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU4503237.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12819..13070)
                     /gene="grxC"
                     /locus_tag="ACHK0Z_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             complement(12819..13070)
                     /gene="grxC"
                     /locus_tag="ACHK0Z_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU4503238.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13212..13643)
                     /locus_tag="ACHK0Z_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             complement(13212..13643)
                     /locus_tag="ACHK0Z_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU4503239.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13888..15432
                     /gene="gpmM"
                     /locus_tag="ACHK0Z_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /gene_synonym="pgmI"
     CDS             13888..15432
                     /gene="gpmM"
                     /locus_tag="ACHK0Z_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU4503240.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15466..16725
                     /gene="envC"
                     /locus_tag="ACHK0Z_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             15466..16725
                     /gene="envC"
                     /locus_tag="ACHK0Z_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU4503241.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16729..17688
                     /locus_tag="ACHK0Z_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             16729..17688
                     /locus_tag="ACHK0Z_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU4503242.1"
                     /translation="MFPFRRNVLAFATLLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17675..>18056)
                     /gene="waaH"
                     /locus_tag="ACHK0Z_RS16365"
                     /old_locus_tag="pgaptmp_003273"
     CDS             complement(17675..>18056)
                     /gene="waaH"
                     /locus_tag="ACHK0Z_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4503243.1"
                     /translation="NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPE
                     FHQQITYEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWS
                     FRLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         4524 a         4654 c         4729 g         4149 t
ORIGIN      
        1 ctgtcattaa tgtaaagcaa catagctcac caataataca atggacaact ctaccggcat
       61 tgatcgctgg tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt
      121 gctgtcactg tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag
      181 ccagaaagta gtttactgaa acaacaagaa gaacataata ataaaattgt gctacttgaa
      241 aaacaacaag cacagctaaa gagtcaactc gaaactattc aaaaacaaca aactggcata
      301 ataaacagta caaagacttt aactcacgta attaaatccg ttaaagacca acaaaatact
      361 tttattttta ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa caacggttcg
      421 gtggctttag cgggtcgagt gttatccatt gacgccacag agaatggtag tgttattcat
      481 atctcactgg tcaacttatt aagtacacct atctcaaaca ttggttttaa tgcgacatgg
      541 ggtggtgaaa aacctgtcga tgccaaagag tttgcccggt ggcaacaatt acttttcaac
      601 acatcaatga aatccacatt gaaattatta ccaggtcaat ggcaagacat taatttgacc
      661 ctgaagggtg tatcgcccaa taacctggga tatctgaagt tagccatcaa catggaaaat
      721 attcagttcg acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg
      781 cagttagtac gcttataaca aggaatagcg agcatcatta atgaacaaaa tatttaaagt
      841 tatctggaac cctgcgacag ggaattatac tgttaccagc gaaacggcaa aaagccgtgg
      901 caagaaatct gggcgcagta agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc
      961 gtcgtttggg gcattggcga atgccgggaa tgacaacggt cagggtgttg attacggtag
     1021 tggatcagct ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa atacttttat
     1081 gaacaccagt ggttccagta ctgctgtggg ttatgacgct atagctgaag gccaatatag
     1141 ctctgccatc gggtcaaaaa cccatgcgat tggcggtgca tcaatggcct ttggggttag
     1201 tgcaatatca gaaggcgata gaagtatagc actgggtgcc tcttcgtatt cattgggcca
     1261 atactcaatg gccctcggcc gttattcaaa agcattgggt aaattgtcta ttgctatggg
     1321 ggactcttcc aaagcggaag gagcaaacgc cattgccctg ggaaatgcca ctaaagctac
     1381 tgagattatg agtattgctc ttggcgacac cgccaatgcg tcaaaagcgt attcaatggc
     1441 gctgggagca agtagcgtcg catctgaaga aaacgcaatt gccctggggc gtagcagtgt
     1501 agctagcggt actgacagcc tcgcatttgg cagacaatca cttgccagcg cagcgaacgc
     1561 tattgcgata ggtgctgaga ccgaagccgc tgaaaatgca actgctattg gcaataatgc
     1621 gaaggcaaaa gggactaata gcatggcaat ggggttcgga agccttgccg ataaagtcaa
     1681 tactatcgca ttaggaaatg gcagccaggc tctggcagat aatgcaatcg ccataggcca
     1741 gggcaacaaa gctgatggcg tggatgccat cgctctgggt aatggtagcc agtcgagagg
     1801 cttaaacacc attgccttag gcacagccag taatgcaact ggtgataaga gtcttgcgct
     1861 tggtagtaat agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag attccattgc
     1921 ggatttagac aataccgtct ctgtcggcaa tagttcatta aaacgcaaga tcgttaatgt
     1981 gaaaaatggc gcgatcaagt ctgacagtta cgatgccatt aatggttcac agctttatgc
     2041 cattagcgac tcggtagcaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
     2101 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
     2161 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
     2221 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
     2281 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
     2341 cgaagccaac accgccaata tcgctactaa taccagcaac attgccacga atacggcaaa
     2401 tattgccacc aataccacca atatcaccaa cctgacggat tccgttggtg accttcaggc
     2461 tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac
     2521 caccagcaaa atcaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt
     2581 taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc
     2641 caataacact tccaatattg ccactaacac caccaacatc tctaacctga ctgagacggt
     2701 gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc
     2761 tcatggcacc gagaccacca gcaaaatcac caacgttaaa gatggcgacc tgacgactgg
     2821 cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa
     2881 taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa
     2941 tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg
     3001 caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc
     3061 cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg
     3121 cggcaatgct tctgtgaata ctgacggtgt gtttaccggt ccaacctaca aaatcggtga
     3181 aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc
     3241 tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac
     3301 taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc
     3361 tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg
     3421 gggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa
     3481 tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc ttgacgacgc
     3541 tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga
     3601 taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga
     3661 cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg
     3721 tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc
     3781 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
     3841 tgagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
     3901 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
     3961 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
     4021 tggtaacacc gacgcaacct atatccaaga aaacggtgcg ggtattaact atgtgcgtac
     4081 taacgacgac ggcttagcgt tcaacgacgc cagcgcacag ggtgttggcg ctacagctat
     4141 aggttataac tctgtcgcca aaggcgatag cagcgtagct attggtcagg gcagctacag
     4201 cgacgttgat acgggtatcg ccctgggtag cagctctgtt tccagccgag tgattgccaa
     4261 aggctcccgt gacaccagca taacggaaaa tggcgttgtt attggttacg acaccacgga
     4321 tggcgaactg ctcggtgcat tgtctatcgg tgatgacggt aaatatcgtc aaatcatcaa
     4381 cgtagccgat ggttccgaag cccatgacgc cgttacggtt cgtcaattgc agaatgcgat
     4441 tggtgcggtc gcaaccacgc cgactaaata cttccacgct aattcaacgg aagaagattc
     4501 actggcagtg ggaactgact cgctggcaat gggtgcgaaa accatcgtga atggcgataa
     4561 aggtattggt atcggttatg gtgcctacgt ggacgcgaat gcacttaacg gcattgccat
     4621 tggtagcaat gcgcaagtca ttcatgtcaa cagtattgcg ataggtaatg gttctacgac
     4681 cactcgtggc gctcaaacca attataccgc ctacaacatg gacgcaccgc agaactctgt
     4741 cggtgaattc tcagtcggta gtgcggatgg tcaacgtcag atcactaacg tcgcagcagg
     4801 ttcggctgat accgatgcgg tcaacgtggg tcagttgaaa gtaacggatg cgcaggtttc
     4861 ccagaatacc cagagcatta ctaacctgga taatcgggta acgaatcttg attcacgcgt
     4921 caccaatatc gaaaacggta ttggcgatat cgtcaccacc ggtagcacca agtacttcaa
     4981 gaccaatacc gatggtgtag atgccagcgc gcagggtaaa gatagcgtcg cgattggttc
     5041 tggctccatt gctgccgcgg ataacagcgt cgcactgggt acagggtctg tggcaaccga
     5101 agaaaatacg atctctgtag gctcatctac taatcaacgt cgtatcacca acgtagccgc
     5161 aggtaaaaac gacaccgatg ctgttaacgt ggcacagttg aagtcttccg aagctggcgg
     5221 tgtgcgttac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
     5281 cggcaacggc ggtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
     5341 ggtgaattac gcgcagttga agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg
     5401 aatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
     5461 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
     5521 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
     5581 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
     5641 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
     5701 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
     5761 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
     5821 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
     5881 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
     5941 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
     6001 ctcattacac gatgtgcgtg gactccagga gacctgcaat gaatctctgg caacaaaact
     6061 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
     6121 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
     6181 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
     6241 cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
     6301 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
     6361 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
     6421 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
     6481 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
     6541 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
     6601 gctttgagat tggccagatg gtggggcggc agctaccgtt tatgaccatt atcgtgctgt
     6661 tctggatcat ggcgattatg gacggctggc gcggtatcaa ggagacgtgg cctgcggtcg
     6721 tggttgcggg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
     6781 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
     6841 gctggcagcc agtgcgcgta ttccgttttg gtgatttggg ggcgtcacag gttgatatga
     6901 cgctggccca caccggttac actgcgggtc aggtgttacg tgcctggaca ccgttcctgt
     6961 tcctgacagc taccgtaaca ctgtggagta tcccgccgtt taaagccctg ttcgcatcgg
     7021 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat aaactggttg
     7081 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
     7141 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
     7201 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctgc
     7261 ccatatactc catcggtatg gtgctggcat tcgcctttat ttcgaactat tccggactgt
     7321 catcaacact ggcgctggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
     7381 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
     7441 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
     7501 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
     7561 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
     7621 gcctgatctt cacctgtata gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
     7681 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
     7741 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
     7801 actggcgatg caactcggcg tatcacgtaa ttcactgcgc gaggcgctgg caaaactggt
     7861 gagtgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
     7921 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaaca ctgatggccg atgatccgga
     7981 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
     8041 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
     8101 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
     8161 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
     8221 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
     8281 cgaacaacat caggctgtca ttgacgccat tttttccggt gatgctgacg gggcgcgtaa
     8341 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
     8401 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
     8461 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
     8521 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
     8581 gtggaagatt tgtcagaagt ggcgctgcgc cagcgtattc tgaaaaacat gtccgactta
     8641 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtggcgct aggtccggta
     8701 ggtttgtgtg gcatgtatgc gcgacgcggc gaagttcagg cagccaaagc ggcggatgcg
     8761 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
     8821 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
     8881 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
     8941 ccgacaccgg gagcgcgtta tcgtgatgcg cattctggaa tgagcggccc gaacgcggca
     9001 atgcgccgct atttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
     9061 ggtcgtccgc atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
     9121 gattacatcg gctggctggg aaataacttc gatccgtcca tctcatggaa agaccttgag
     9181 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
     9241 gcgcgcgatg cagtacgttt tggtgctgat gggattgtgg tttctaacca cggtggccgc
     9301 cagctggacg gtgtactctc ttccgctcgt gcactgcctg ctattgcaga tgcggtgaaa
     9361 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
     9421 attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcttgtatgc gctggcaaca
     9481 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
     9541 atgacgctga ctggcgcgaa atcgatcaat gaaattacgc aggattcgct ggtgcagggg
     9601 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
     9661 tttgagcccc ctcaccctaa ccctctccct cagggagtgg ggaccgttcg gcgctgtatg
     9721 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggtga
     9781 ggggaaaagg ccgcactgct caccaatttt ctgctatcct gcccccgcac taagggggca
     9841 gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc aatatcatcc
     9901 gtctttgcgc taataccggc tttcgtctgc atatcatcga accgatggga tttgcctggg
     9961 acgataagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc
    10021 atcatgactt tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca
    10081 cgaaaggtac gcctgctcac agcgccgtaa actatcagga tggcgattat ctgatgttcg
    10141 gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa
    10201 ttcgcattcc gatggtgccg gacagccgca gcatgaacct gtccaatgcg gtgtcagttg
    10261 tggtgtatga agcctggagg cagctgggat atccgggagc gttgttgaga gattagttac
    10321 tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatc agatcccatc
    10381 cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt
    10441 atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg tatgcggcgg
    10501 caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt
    10561 gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc
    10621 accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc
    10681 aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc
    10741 aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa
    10801 gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa
    10861 ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcaactg ccgggtcgcg
    10921 ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta
    10981 ggcttcttcc accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt
    11041 cgccagcatg tagctcagtg cactaccaag gttttcgtgc ttgagtagcg tcgcgtggta
    11101 aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt
    11161 ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg
    11221 gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtggctgc
    11281 tgcgctcgtc cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc gcgttttttc
    11341 cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg aagcgatgcg
    11401 ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct
    11461 gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg ttacgcgact
    11521 ggttgtcggt acaggtaagc accagatcgc caagccccgc catgcccata aaggtggcag
    11581 gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg gtgatcagcg
    11641 ccgtacgcgc attcgcacca aaaccgatac cgtcggacat ccccgcgcca atggcaatga
    11701 cgtttttcac cgcaccgcca agctgcacac cgatgaaatc cgggttgctg taaacgcgga
    11761 aacttttgcc acagtgcagc aattgttgga gatcatcggc aaaggtctgg tcggtagagg
    11821 ccaacgaaat agccgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga
    11881 taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc
    11941 cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg
    12001 gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac
    12061 ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca
    12121 catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt
    12181 catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca agagcggtgc
    12241 cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg
    12301 atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa
    12361 gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc tggtgatgca
    12421 ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg
    12481 ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc
    12541 ggtttcttcg cccagggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc
    12601 tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg
    12661 gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat
    12721 ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt
    12781 ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag gggatccagt
    12841 ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata
    12901 aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg cttggcggca
    12961 ttgccatcga tcggcagctc ctggaaactc acgcccttgc tgctcagcag tgcttttgca
    13021 cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat aaattagctc
    13081 ctgttttttt accctgaaat ttcatgttgc agggcggcag caaaatggag tgtccccaga
    13141 agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga
    13201 cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca gccagcaacg
    13261 ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc
    13321 tggcactgca tgccagaacc gtctaccacg ataaccggtt tgtctttgtg cttctcaagc
    13381 tcaccaacat tgttggcttt gatttcactc ggcaacagat tgatagaacc tgcgatatgg
    13441 cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc
    13501 agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta
    13561 gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca
    13621 acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac
    13681 caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca
    13741 attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc
    13801 gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt
    13861 ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg
    13921 attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa
    13981 accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt
    14041 ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg
    14101 ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc
    14161 gctttctttg ctaatccagt gctgactggc gcggtagata aagcgaaaaa cgcaggtaaa
    14221 gcagtacaca ttatgggtct gctctccgta ggcggcgtac acagccacga agatcacatc
    14281 atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc
    14341 cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag
    14401 aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg
    14461 gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag
    14521 ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa
    14581 aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg
    14641 gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact
    14701 cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat
    14761 ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct
    14821 tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt
    14881 tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg
    14941 ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa
    15001 ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa
    15061 tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg
    15121 gaagcggcag ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg
    15181 gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg
    15241 cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt
    15301 tatgttggtg ataagaacgt gaaagcggtt gaaggcggca aactttctga catcgcgccg
    15361 accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc
    15421 atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg
    15481 aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta
    15541 ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc
    15601 gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc
    15661 gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg
    15721 caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa
    15781 ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cgcaactgga tgccgcattc
    15841 cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag ccagcgtgga
    15901 cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag
    15961 ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga gaaacaaagc
    16021 gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac tcaggcgctg
    16081 aacgagcgta aaaagacgct ggcagggctg gagtcttcca tccagcaagg tcagcaacag
    16141 ttgagcgagc tgcgcgccaa cgaatcccgt ctgcgtaaca gcattgcccg tgcggaagcc
    16201 gcggcgaaag cgcgtgcaga acgagaagca cgtgaggccc aggcggttcg cgaccgccag
    16261 aaagaagcga cgcgtaaagg caccacctac aaaccgactg aaagcgaaaa atcgctgatg
    16321 tcccgtaccg gtggtctggg cgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg
    16381 acgctgcatc gctatggcga acagctacag ggtgaattac gctggaaagg gatggtgatt
    16441 ggtgcctctg aaggtactga agttaaagcg attgccgacg gtcgggtgat tctggctgac
    16501 tggctgcaag gctacggtct ggtggtggtg gttgagcatg gtaaaggcga catgagtctt
    16561 tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc gggccagcca
    16621 attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt cgaaattcgc
    16681 cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt gtttccattt
    16741 cgtcgtaacg ttcttgcatt tgccactctg ttggcgctct cctcccccgt acttgctggc
    16801 aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg
    16861 gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg
    16921 gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctgccaat ggcaccgttg
    16981 agtaaacagc cgctggagaa aaatacgctg cgcccggaga tgagcagcga cgaaattgag
    17041 cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa caaccacatg
    17101 ggtagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc gctggagcgt
    17161 tacaatcttt acttcctcga cagtgtaacc attggtaata cccaggcgat gcgcgccgcg
    17221 caaggcactg gcgtgaaggt tattaaacgg aaggtgttcc tcgacgattc acagaacgaa
    17281 gcggacatcc gtgtgcagtt caatcgcgca attgatctgg cgcgtcgcaa cggttcaacc
    17341 attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat ggtttataac
    17401 ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat
    17461 acgtctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt ccgtggcgtg
    17521 aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt ctttgaagta
    17581 ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca gtggcaaggc
    17641 tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag gcgctacgcg
    17701 taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa gaccacagta
    17761 acgcctggta acccaccttc acgctgcgca cattggtaat caggcgctta tacataccgg
    17821 aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcgcaccg
    17881 catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga taaatcgtaa
    17941 ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta atatagtgac
    18001 gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac gtatta
//
LOCUS       DAZFWP020000100        17979 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFWP020000100 DAZFWP020000000
VERSION     DAZFWP020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.100"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..71
                     /locus_tag="ACHK0Z_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             <1..71
                     /locus_tag="ACHK0Z_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503244.1"
                     /translation="IIDRRLQMRTNDFYSLLNRKYL"
     gene            110..327
                     /locus_tag="ACHK0Z_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /pseudo
     CDS             110..327
                     /locus_tag="ACHK0Z_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            437..700
                     /locus_tag="ACHK0Z_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /pseudo
     CDS             437..700
                     /locus_tag="ACHK0Z_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            924..1049
                     /locus_tag="ACHK0Z_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /pseudo
     CDS             924..1049
                     /locus_tag="ACHK0Z_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1109..1615)
                     /gene="yciE"
                     /locus_tag="ACHK0Z_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             complement(1109..1615)
                     /gene="yciE"
                     /locus_tag="ACHK0Z_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4503245.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKIAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1661..2161)
                     /gene="yciF"
                     /locus_tag="ACHK0Z_RS16395"
                     /old_locus_tag="pgaptmp_003279"
     CDS             complement(1661..2161)
                     /gene="yciF"
                     /locus_tag="ACHK0Z_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4503246.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2247..2426)
                     /gene="yciG"
                     /locus_tag="ACHK0Z_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             complement(2247..2426)
                     /gene="yciG"
                     /locus_tag="ACHK0Z_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4503247.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(2807..3613)
                     /gene="trpA"
                     /locus_tag="ACHK0Z_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             complement(2807..3613)
                     /gene="trpA"
                     /locus_tag="ACHK0Z_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU4503248.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(3613..4806)
                     /gene="trpB"
                     /locus_tag="ACHK0Z_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             complement(3613..4806)
                     /gene="trpB"
                     /locus_tag="ACHK0Z_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU4503249.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     AREEI"
     gene            complement(4818..6179)
                     /gene="trpCF"
                     /locus_tag="ACHK0Z_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             complement(4818..6179)
                     /gene="trpCF"
                     /locus_tag="ACHK0Z_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU4503250.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(6180..7775)
                     /gene="trpD"
                     /locus_tag="ACHK0Z_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             complement(6180..7775)
                     /gene="trpD"
                     /locus_tag="ACHK0Z_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU4503251.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(7775..9337)
                     /gene="trpE"
                     /locus_tag="ACHK0Z_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             complement(7775..9337)
                     /gene="trpE"
                     /locus_tag="ACHK0Z_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU4503252.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9359..9452)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9429..9473)
                     /gene="trpL"
                     /locus_tag="ACHK0Z_RS16430"
                     /old_locus_tag="pgaptmp_003286"
     CDS             complement(9429..9473)
                     /gene="trpL"
                     /locus_tag="ACHK0Z_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU4503253.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            9611..10492
                     /gene="rnm"
                     /locus_tag="ACHK0Z_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             9611..10492
                     /gene="rnm"
                     /locus_tag="ACHK0Z_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU4503254.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10489..11109
                     /gene="yciO"
                     /locus_tag="ACHK0Z_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             10489..11109
                     /gene="yciO"
                     /locus_tag="ACHK0Z_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU4503255.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            11210..12082
                     /gene="rluB"
                     /locus_tag="ACHK0Z_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             11210..12082
                     /gene="rluB"
                     /locus_tag="ACHK0Z_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU4503256.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(12122..12712)
                     /gene="cobO"
                     /locus_tag="ACHK0Z_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             complement(12122..12712)
                     /gene="cobO"
                     /locus_tag="ACHK0Z_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4503257.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(12709..13467)
                     /gene="yciK"
                     /locus_tag="ACHK0Z_RS16455"
                     /old_locus_tag="pgaptmp_003291"
     CDS             complement(12709..13467)
                     /gene="yciK"
                     /locus_tag="ACHK0Z_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU4503258.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13687..14736
                     /gene="sohB"
                     /locus_tag="ACHK0Z_RS16460"
                     /old_locus_tag="pgaptmp_003292"
     CDS             13687..14736
                     /gene="sohB"
                     /locus_tag="ACHK0Z_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU4503259.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14772..15023)
                     /gene="yciN"
                     /locus_tag="ACHK0Z_RS16465"
                     /old_locus_tag="pgaptmp_003293"
     CDS             complement(14772..15023)
                     /gene="yciN"
                     /locus_tag="ACHK0Z_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU4503260.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15403..>17979
                     /gene="topA"
                     /locus_tag="ACHK0Z_RS16470"
                     /old_locus_tag="pgaptmp_003294"
     CDS             15403..>17979
                     /gene="topA"
                     /locus_tag="ACHK0Z_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU4503261.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGGALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGK"
BASE COUNT         4422 a         4454 c         4687 g         4416 t
ORIGIN      
        1 agattatcga tcgacgatta cagatgcgta caaatgattt ttattcatta ttaaacagaa
       61 aatatttata agtgctatat tcatgaatga aaaatagcta tatgcgtaca ttctcaacct
      121 gcccgtcacg tagcaggttg aatcactttt tcttgataaa gggctggtcc tgccactgtt
      181 gttgttgggt gcgttaaaaa tacactacta atatgcccgg catgcgggca acagagtcct
      241 gttcacgatc gccgccaccg gaaatggcgc tgcctcgata cctgccagtt catggcacta
      301 ttcgaagccg atgttcccca cgttatatgg agtaatcatt ggggttatgc cgcagcacgt
      361 tgtatgcaag aatgtgctgc ggctggtttg tgagctttcg atagtggttg ttatttttgc
      421 ccttatttgt tccggaggcc ctggttcaat ggtccgtctg ccccctgtgg tgatgtcagc
      481 aaaatcagcc actgcgcgaa ccacaatagc ccgggaagat gctgaagatc accaggtaaa
      541 gctgtcagcg cagaaactgg aagaactgct cgcatcaatg gttaaggatg aggttgatcg
      601 caatgatggg atttattgac gtcagcgaga gcagaaggaa gaactgaata acctgaatga
      661 tttacgctca atcagagcga tgatgattgg cagtaattag cggtcaattt cgggtttttt
      721 ggctgtgcgc tccccgccca gcattgcgcg tcaattagcc ccaaaaaaaa cgaaaaagtt
      781 acaatcaagt aaaaaattcc ttctgcaaaa ctaaaaaaga tttcaataaa atcaggcagt
      841 taaaaacata aaaaaaaggt acaaaaaaac atctcaaagt cttgtttttt gcttcaaata
      901 caattggtta ttgtgttttt tgaaacctac cagtgtggta catggatatc gataccaccg
      961 ccaattataa gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt
     1021 ttatgttctc agcaggatat cgtttttaat tccacaaaaa aacgaccccg taatatacgg
     1081 ggtcaataag gacatggcat aaagtgtatt atttcttcgc ttctacgccg tcagtttcag
     1141 agcgaattaa aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt
     1201 gcttttcctc atttaaaatc gcttcgatgg ttggaattga agctgtatca ccggcatttt
     1261 ttgctgctgc taatagtgag gtgtaacagg cgatttcaaa ttgctcgaag acatatccgc
     1321 taatagagcc tttgactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg
     1381 cagctatttt actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa
     1441 tagtttccag ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc
     1501 gtagttcagg ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct
     1561 tttccattgc gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa
     1621 tgtcctcttt ataaaatgac taaaagttaa agttcatatt tcaggcttta ttttcggctt
     1681 tcttatttac gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt
     1741 ccagggtctc tttcagaagc ttcgctgctt tacgataacc taattgttca gccagcgtcg
     1801 ctaatgtccc gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg
     1861 cggcatcacg cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa
     1921 gaccttccat tgccacacat ttcatgcgct taattttcag attcgattct gattccacaa
     1981 cttgatcaat acgttcaatc tgtccatgtg tttcctcgag gtgcgcatga aaagcctgac
     2041 ttaatttttc gtttgatgtt gctcttgcga gttttgccag tgcccgggtt aattgttttt
     2101 ctgcgctgta ggtatctgaa agcaggtgaa taaatacatc ttcaatggtc ttcatattca
     2161 tatttttctc cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc
     2221 agctgatatt aataattaac ggtgaatcag gatttgcctg atttattacc accgctttgt
     2281 tgaccgcctt ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg
     2341 ctatgctgac cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt
     2401 cctgaaccac cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta
     2461 atatgagtgt tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt
     2521 aagcgcaacg agaagataga gggaaaatat attttgagga acattctgga tatattaaca
     2581 attacctgag gaataagtga cttagagaaa attattgatt ttactggtgt tatgttgcgg
     2641 gattaatttg tttgattata ttactgttgg gcggaaaaat gacgtaagtt gacgttcgac
     2701 cggggtaagc gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc
     2761 attaaagaaa gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg
     2821 ccgctttcat cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa
     2881 tatgttgctc gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat
     2941 caatcgctgc ttttacctga tccggggcgg aaataccaaa cccctgcaat ggaggtgcag
     3001 cgttgtactc tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg
     3061 cgccggtcac gcccgctcgt gacagcaaat aggtgtaacc acgaccgtaa gaggctatct
     3121 ggcgcagcag gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatggc
     3181 gcaacgcggc ctggcggaag ggcgcggact cttcaattgg cacatcggca accagcaccg
     3241 aatcgacgcc gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca
     3301 gattggcata catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca
     3361 gcatttcaaa acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt
     3421 gaatggtcgg gccatccgct agagggtcgg agaaggggat acctaactcc agcgcgtcag
     3481 caccggcttc aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga
     3541 gcgtgacgaa aggaacgaat gcgccttctt tgcgctcctt caactgggca aacagagatt
     3601 cgtagcgttc catcagattt cctctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc
     3661 tttatcgccg cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg
     3721 catcattttc aacgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc
     3781 gtgcaggcac agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc
     3841 agcgcgtcca gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc
     3901 ggcggagatg gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt
     3961 cataccgaaa tagatgccca cgcgaccatg ttttaacggt gcgccgtgct cgccagtttc
     4021 gataccgtga ccacctggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc
     4081 agcaaacatg ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag
     4141 gcgaccttct ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc
     4201 acgcacaatg gtcggataag gatgcgggcc agctgcggtg cccagcatat agtgcgcggt
     4261 ttcgtaacta ccggaccagt cgcgcagcgc ctcgttacag gcatctttca gcgtcgcgga
     4321 accgctatgc accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga
     4381 ctggcgttct acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct
     4441 ggcaagggcc gacgccacgc catgctgacc ggcaccggtt tcggcgatga tttcggtttt
     4501 acccatccgc ttcgccagta aagcctgacc gagcacctgg ttagttttat gcgcgccgcc
     4561 gtgcagcaaa tcttcacgct tgagatacag agtggtgttc gtcccggctg taatgttctg
     4621 gcatttggtc agcgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgagc
     4681 ctgaaattca ggatcttttt gcgcactgac aaaagcttct tccagctggc gcagagcagg
     4741 catcaggatt tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt
     4801 tgtcattgtt cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt
     4861 gcgtctttga tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg
     4921 gtttgtgccg cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca
     4981 agcgattgac catttaatag tgaccagtcg aaacgttgcc cgcttccacc ctggccgttg
     5041 tctaaaacat atttatcaac gtgctgaagc tcgcgggcgg gcagggtttc accgacgctt
     5101 aatgctttcc agatggcaac gtgtgctggc agagcttcac gcagcgtatc gatatacagc
     5161 tgatcttcat taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc
     5221 acatcggcaa tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc
     5281 acttcctgcg cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca
     5341 ccgtaaatcg cgcccgcgtc ataagctgct ttagcgtctt gcccacgcgt caggccacat
     5401 actttattct cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc
     5461 aacgccgaac caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa
     5521 gtattgatgc cggattcgct gattaccgtt acgttgtgcc ccagtttcgg cgcaagctcg
     5581 cgggtacggt tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc
     5641 tttgccccca atgcaatggc acgctccagt tcctcttcat tactgacttc ggtcagcaca
     5701 cccatctcca gactgtgggc gacggcggca agctggcgat attgttcgtc atccagtact
     5761 gaaagcatta ataagcaggc atcagcctgg taatagcgcg ccagatagat ctggtaagga
     5821 tcgataatga agtctttaca taaaatcggc tgcggggcga tttggctgac gatggggagg
     5881 aaatcaaagc tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa
     5941 tgtttataaa tggcggcaat gcgtgctgga tcgaaatcat cacggatcac gccttttgac
     6001 ggcgacgctt tcttgcactc cagaataaac gccgtgcgcg caccctgtag cgcatcataa
     6061 aaatgtcgcg tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg
     6121 cgggtttcta cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt
     6181 taccctcgtg ccgccagtgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca
     6241 agaacggttt gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg
     6301 acgttcgcag cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt
     6361 aaaatgtcac ggttttcttc cggtgttccg cctgccagtt gctcctggtg gtagggagtc
     6421 aggccaaaat cttcagcggt caattgatag ctcttaattt cgccgtcatg cagctcggca
     6481 acgattgtcg gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg
     6541 cgttgatacc ccagcacgcg caaggtttcg gcaatcggca gcaccagttc cggactataa
     6601 acaccaatta acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac
     6661 agggtgcggg ttttcagttg ctggcgaacc ggcatcgcgt ggcggaatcc ggtgtgatac
     6721 ttcggcgcaa agaggaaaca tacacctaac tcatccagcg cctggcgcga tttatcggcg
     6781 ttcatatcaa gattaatacc gaacgccgcc agcagatcgg acgaaccaga tttactggag
     6841 acgctacggt tgccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg
     6901 gcggtagaaa tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac
     6961 agataatccg ggcgcgggaa cggcgcggcg ttttccagta gcgcggttgc tgctccggcg
     7021 atctcgttcg ggtgctcacc gcgaattttc atgctcacca gcgccgccgc cagttgttcc
     7081 ggcttcagct cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc
     7141 gtctgcgcct gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc
     7201 tgctgcgccc aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat
     7261 tccggatgga actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg
     7321 ccattaaaat gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga
     7381 taacgcgcca ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca
     7441 atgctcgacg ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct
     7501 tcgacaattg cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg
     7561 gtgaggagtt ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc
     7621 agtaccggat tgctcatcgt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg
     7681 cggtaaatca ccacattatg cccattgctg cgcaactgat ctgccaggtt gtacgtaaaa
     7741 gagtcgatat tatcgagcag cagaatgtca gccatcagaa agtctcctgt gcatgatgcg
     7801 cggtggcaat agcgcgcagt acagcgcggg ctttattacg ggtttcgtcg gcttccgact
     7861 gcggaacaga atcaaggact acgccagcac cggcttgcac ggtggcgata ccgttttcca
     7921 ccagcgccga gcggatcaca atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta
     7981 ccgcgccgcc gtagctgccg cggcggcgac cttccgcctc ggcaattaac tgcatagcgc
     8041 gtactttcgg cgcaccactt aacgttccca tattcataca ggcgcggtaa gcgtgcaggg
     8101 cgtcgagatc gtggcgcagc tcaccgacca cgcgggagac caagtgcatc acgtaagagt
     8161 aacggtcaac tttggtgagg tcggcgacgt agcggctgcc gggggtgcaa atgcgtgcca
     8221 gatcattacg ggcgagatcc accagcatca gatgttcaga aagctcttta tgatcggtac
     8281 gcatttccag ttcgatgcgg ctgtcgaggt ctctgtccag cgagccatcg gcacgacgac
     8341 cgcgtggacg tgttccggca atcgggtaga tctcaatctg gcggctggtg gcgtcatact
     8401 tgagcgaact ttccggcgac gcgccaaaca gggtgaaatc attatcctgc ataaaaaaca
     8461 tgtacgggct gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg
     8521 gcagagagaa acggcgagat ggcaccacct ggaaaatttc tccagcgcga atcgcttttt
     8581 gcaacaaacg cactacgcca ccgaactctt catcgctctg attacattca caacgcatat
     8641 gcggcacgga aaccactggc agcggcggcg cggcttcggt cagttgctga cgtagttcgt
     8701 tcaggcgagc agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa
     8761 tacgggtgct ttttttctga tggtcaatca ccatcagagt ttcagcgaga taaaaacaga
     8821 aatcagggca gttattttcc gctgacagtt gcggtaaatc ttcaaatccc gccacaaggt
     8881 cataagagaa caggccgccg aagaacattg cttctcgttc ttccttcggt acattcaaca
     8941 gattctgcaa taaacggaaa gcgtcaaaaa ccgaaaggga gcataagcga gcgtcttcat
     9001 ccagcagtgg actgacaggg gggaagcgca gcacacggca gtttggtaat tgttcatttt
     9061 ccacacccgc aggcagggcg ttatccagta gtgtcagcag ggcttcgccg ttgccggaaa
     9121 gtgcctggat tgtgacagtg tcacctaaag ctgtaatgcg cagcgcactg tctaccagca
     9181 gcaggctttt taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg
     9241 gacgatcccc acacaactgg tgaaaaagcg cggtgggatt gtcgcgataa gcgccttcgc
     9301 aggttagcag ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa
     9361 aaaaaagccc gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac
     9421 tgcccgtttc aggaagtgcg ccaccaacct ttcagtacga aaattgcttt cattgtcgat
     9481 acccttttta cgtgaacttg cgtactagtt aactagttcg atgattaatt gtcaacagct
     9541 catttcagaa tatttgccag aaccgttatg atgtcggcgc aaaaaacatt atccagaacg
     9601 ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac
     9661 agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt
     9721 cggcaccctg gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga
     9781 aatttcacgt tcgggactgg cgttgaatct tattcccggc gtggaaattt ccacggtctg
     9841 ggaaaatcat gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg
     9901 tgagttcctc gcgcagcaga cagaacggcg caatcagcgg gcgcagctga ttgccgaaag
     9961 acttgaaaaa gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg
    10021 cgcagtgacg cgcggtcatt ttgcgcgttt ccttgttgag tgcggcaaag ccagttcaat
    10081 ggcggatgtc tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg
    10141 gtgtacaata gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc
    10201 tcatcctggg cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc
    10261 cgaacaccac ggtgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgtac
    10321 ccagctggcg gcccttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca
    10381 tcagccatgc ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg
    10441 cgtctggcag ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt
    10501 ttttatattc atcctgataa cccacagcaa cgtctgatca accaggcggt ggagatcgtg
    10561 cgtaaaggcg gggtgattgt ttatccaact gattccggct atgcgctcgg ctgtaaaatt
    10621 gaagataaaa acgcgatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac
    10681 tttaccctga tgtgtcgcga tctttctgaa ctctcgacct attcatttgt tgataacgtt
    10741 gcgtttcgtt taatgaaaaa caacacgccg ggcaactata ccttcatctt gaaggggacg
    10801 aaggaagtgc cacgccgtct gttgcaggaa aaacgcaaaa ccatcggtat gcgtgtgcct
    10861 tctaacccga ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg
    10921 ctaatgctgc caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg
    10981 gaaaaacagg tggatttgat tatccacggc ggctatctcg gacagaaacc gacaacggtt
    11041 attgatctta ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct
    11101 ttcttataag tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca
    11161 ttgattctcc tgttcgacgc ctgtgaaggc gacaccaaaa ggaagctcta tgagcgaaaa
    11221 attacagaaa gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat
    11281 tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt
    11341 tacccctggg ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga
    11401 acaaatttgt cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga
    11461 tccggaaggg cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat
    11521 tgccgtaggt cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga
    11581 actggcaaac cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt
    11641 atttggtcag gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga
    11701 tggtccggca gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta
    11761 caacgtgact ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg
    11821 tgtgcaggtg agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct
    11881 gccacgcggt ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt
    11941 ggagcttccg ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa
    12001 ggctaatcag attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc
    12061 tggcgggcgt aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag
    12121 attaataatc aatccctatt tgcgccttaa caccggcatc aaacgcatgt ttgatggggc
    12181 gcagctcact taccgtatcc gccagttcaa gaatatcccg atgacagcct cgacccgtga
    12241 taatcaccgt ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca
    12301 aatagtcata cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat
    12361 cagcaagcat ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc
    12421 ggttttgtgt atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct
    12481 ccagcagatt gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcctactt
    12541 tttttccgtg accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc
    12601 cattgccggt aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat
    12661 ctactttttc tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat
    12721 tcctggttta cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc
    12781 gcccatcagc cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc
    12841 ttcggtcggg aaggcgctgg cacgcattgc ggtgcgcgta ccgcctgggt taatgcagtt
    12901 gacgcgcagg cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa
    12961 tttcgacgct gcatatgcgc cccagttggc tcgtccctga cgtccaacgc ttgatgaagt
    13021 aaagaccagt gaaccggcgt ccgatttgag taataaagga agcagtgcct gggtgagcat
    13081 aaaggtggca ttaacgttga cctgtatgac gtcctgccag acctgtggat tttgttcgct
    13141 cattgggcaa acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata
    13201 attaacggta atgcgctgtg ccagttgttg gcaatcttcg gacgtgcagg tcagcaaatc
    13261 gagaataaac cactgtggct gacgtccagt ttcttcgttt atgtggctgg ctacctgacg
    13321 taatttttct tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat
    13381 cgcggcttca cggccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag
    13441 taaatcttgt tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgtgctg
    13501 ccagtgtact ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc
    13561 tggggcgata acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg
    13621 cgagacggct taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg
    13681 ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg cgaaaatcgt taccgttgtg
    13741 ctagcgattg cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag
    13801 cgtggcgagt tacgggtcaa caatctcagc gaacagtata aggagatgaa agaagaactg
    13861 gccgcggcgc tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag
    13921 cacaagcaag aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac
    13981 agtaaacctc gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac
    14041 tcgctacgtg aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg
    14101 ctccgtctgg aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg
    14161 cagcgtctgc gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc
    14221 ggcggttaca tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg
    14281 ggttccattg gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat
    14341 attgatatcg aactgcacac tgccgggcag tataagcgta ctctgacgtt gctgggtgaa
    14401 aataccgaag aagggcggga gaaattccgc gaagagttga acgaaacgca tcagttgttt
    14461 aaagattttg tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa
    14521 cactggtacg gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat
    14581 gaagttattc ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt
    14641 aaacgactca ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta
    14701 cgctggtggc agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct
    14761 cgcgttttgc tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg
    14821 aacatattaa acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag
    14881 tcgccggtaa ataccagcac gccattacgc tgcgtgacgc cggtggcttc aatccctgcg
    14941 agcgtatcct catgctcacg aatgattttg ttggcctctt tcaacagcgt ttcgcggtcg
    15001 atgggttgtg tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag
    15061 gcatggcttg cagacaaata taccacgctg gtggcaagag cgccttactg gtaactttgg
    15121 attttgcatg ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt
    15181 caatctggca atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt
    15241 aggccaagac ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggt
    15301 tgatatccgc agagaacgag tccatatcgg taactcgttg ccagtggaag gtttatcaac
    15361 gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc
    15421 atcgttgagt ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg
    15481 gtgaaatcca gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag
    15541 agtgccgact ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct
    15601 ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg
    15661 cctggtaaag agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc
    15721 tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg
    15781 attggtggtg atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg
    15841 atccgccagg catttaacaa accgggtgag ctgaatattg atcgtgttaa tgcccagcag
    15901 gcgcgtcgct ttatggaccg cgtggttggg tatatggttt cgccgctgct atggaaaaag
    15961 atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg cggttcgcct ggtggtcgag
    16021 cgtgagcgtg aaattaaagc gttcgtgccg gaagagttct gggaagtcga tgccagcacg
    16081 accacgccat ctggtggagc gttggcgttg caggtgactc atcagaacga caaaccgttc
    16141 cgtccggtca acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac
    16201 agcgtgctgg aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc
    16261 tctacgctgc aacaagctgc cagcacccgt cttggatttg gcgtgaaaaa aaccatgatg
    16321 atggcgcagc gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac
    16381 ctgagtcagg acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa
    16441 tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcatgaa
    16501 gcgattcgtc cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat
    16561 gcgcagaaac tgtaccagtt aatctggcgt cagttcgtag cctgccagat gaccccagcg
    16621 aaatatgact ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt
    16681 cgtattttgc gttttgatgg ctggacaaaa gtgatgcctg cgttgcgtaa aggcgatgaa
    16741 gatcgcatct taccagcagt taataaaggc gatgctctga cgctcgttga acttacacca
    16801 gcccagcact ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagagctg
    16861 gaaaaacgtg gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat
    16921 cgtggctacg tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc
    16981 accgatcgcc ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg
    17041 gaaaacagcc tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac
    17101 ttcttctcgg atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt
    17161 atgcgcccga accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg
    17221 gggattcgca cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg
    17281 aaagagcgtt gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg
    17341 gaaggcgaag atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc
    17401 acggcgatgg acagctatct catcgatccg aaacgtaagt tgcatgtctg tggtaataac
    17461 ccaacctgcg acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc
    17521 ccgatcgttg agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt
    17581 aaatacatgg cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt acgtaacggc
    17641 gaagtggcac caccgaaaga agatccggtg ccattacctg agctgccgtg cgaaaaatca
    17701 gatgcttatt tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc caacactttc
    17761 ccgaaatcgc gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt
    17821 ctgccggaaa aactgcgtta tctggccgat gcgccacagc aggatccgga aggtaataag
    17881 accatggttc gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga
    17941 aaggcgactg gctggtcagc attttatgtt gatggcaaa
//
LOCUS       DAZFWP020000101        17976 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFWP020000101 DAZFWP020000000
VERSION     DAZFWP020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.101"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            58..1473
                     /gene="tnaA"
                     /locus_tag="ACHK0Z_RS16475"
                     /old_locus_tag="pgaptmp_003295"
     CDS             58..1473
                     /gene="tnaA"
                     /locus_tag="ACHK0Z_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU4503262.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1564..2811
                     /gene="tnaB"
                     /locus_tag="ACHK0Z_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             1564..2811
                     /gene="tnaB"
                     /locus_tag="ACHK0Z_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU4503263.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2943..4118
                     /gene="mdtL"
                     /locus_tag="ACHK0Z_RS16485"
                     /old_locus_tag="pgaptmp_003297"
     CDS             2943..4118
                     /gene="mdtL"
                     /locus_tag="ACHK0Z_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU4503264.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4093..5052
                     /gene="yidZ"
                     /locus_tag="ACHK0Z_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             4093..5052
                     /gene="yidZ"
                     /locus_tag="ACHK0Z_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU4503265.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5209..5958
                     /locus_tag="ACHK0Z_RS16495"
                     /old_locus_tag="pgaptmp_003299"
     CDS             5209..5958
                     /locus_tag="ACHK0Z_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU4503266.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5980..6546
                     /gene="yieF"
                     /locus_tag="ACHK0Z_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /gene_synonym="chrR"
     CDS             5980..6546
                     /gene="yieF"
                     /locus_tag="ACHK0Z_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU4503267.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6600..7937)
                     /gene="adeP"
                     /locus_tag="ACHK0Z_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             complement(6600..7937)
                     /gene="adeP"
                     /locus_tag="ACHK0Z_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU4503268.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8104..8769
                     /gene="yieH"
                     /locus_tag="ACHK0Z_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             8104..8769
                     /gene="yieH"
                     /locus_tag="ACHK0Z_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU4503269.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8836..9309
                     /locus_tag="ACHK0Z_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             8836..9309
                     /locus_tag="ACHK0Z_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503270.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9358..9945
                     /gene="cbrC"
                     /locus_tag="ACHK0Z_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             9358..9945
                     /gene="cbrC"
                     /locus_tag="ACHK0Z_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HGU4503271.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10007..10729)
                     /locus_tag="ACHK0Z_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     CDS             complement(10007..10729)
                     /locus_tag="ACHK0Z_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4503272.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10744..11913)
                     /locus_tag="ACHK0Z_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             complement(10744..11913)
                     /locus_tag="ACHK0Z_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4503273.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11940..13556)
                     /gene="bglH"
                     /locus_tag="ACHK0Z_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     CDS             complement(11940..13556)
                     /gene="bglH"
                     /locus_tag="ACHK0Z_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HGU4503274.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNAIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13642..15036)
                     /gene="bglB"
                     /locus_tag="ACHK0Z_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             complement(13642..15036)
                     /gene="bglB"
                     /locus_tag="ACHK0Z_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HGU4503275.1"
                     /translation="MKAFPETFLWGGATTANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVSKTRGLSLKK"
     gene            complement(15055..16932)
                     /gene="bglF"
                     /locus_tag="ACHK0Z_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             complement(15055..16932)
                     /gene="bglF"
                     /locus_tag="ACHK0Z_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGU4503276.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17065..17901)
                     /gene="bglG"
                     /locus_tag="ACHK0Z_RS16550"
                     /old_locus_tag="pgaptmp_003310"
     CDS             complement(17065..17901)
                     /gene="bglG"
                     /locus_tag="ACHK0Z_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGU4503277.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4528 a         4501 c         4257 g         4690 t
ORIGIN      
        1 ttgcagatgt aatattcaca gggatcactg taattaaaat aaatgaagga ttatgtaatg
       61 gaaaacttta aacatctccc tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt
      121 actactcgcg cttatcgtga agaagcaatt attaaatccg gtatgaaccc gttcctgctg
      181 gatagcgaag atgtgtttat cgatttactg accgacagcg gcaccggggc ggtaacccaa
      241 agtatgcagg cagcgatgat gcgcggcgac gaagcctaca gcggcagccg cagctactat
      301 gcgttagccg agtcagtgaa aaatatcttt ggttatcaat acaccattcc gactcaccag
      361 ggccgtggcg cagagcaaat ttatattccg gtactgatta aaaaacgcga gcaggaaaaa
      421 ggcctggatc gcagcaaaat ggtggcattc tctaactatt tctttgatac cacgcagggc
      481 catagccaga ttaacggctg taccgtgcgt aacgtctata tcaaagaagc cttcgatact
      541 ggcgtgcgtt acgactttaa aggtaacttt gaccttgagg gattagaacg cggtattgaa
      601 gaagttggcc cgaataacgt gccgtatatc gttgcaacca tcaccagtaa ctctgcaggt
      661 ggtcagccgg tttcactggc aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat
      721 attccggtgg taatggactc cgcacgcttt gctgaaaacg cctatttcat caagcagcgt
      781 gaagcagaat acaaagactg gaccatcgag cagatcaccc gcgaaaccta caaatatgcc
      841 gatatgctgg cgatgtccgc caagaaagat gcgatggtac cgatgggcgg cttgctgtgc
      901 atgaaagacg acagcttctt tgatgtgtac accgagtgca gaaccctttg cgtggtgcag
      961 gaagggttcc cgacatatgg cggcctggaa ggcggcgcga tggagcgtct ggcggtaggt
     1021 ctgtatgacg gcatgaatct cgactggctg gcttatcgta tcgcgcaggt acagtatctg
     1081 gtcgatggtc tggaagagat tggcgttgtc tgccagcagg cgggcggtca cgcggcattc
     1141 gttgatgccg gtaaactgct gccgcatatc ccggcagacc agttcccggc acaggcgctg
     1201 gcgtgcgagc tgtataaagt cgccggtatc cgtgcggtag aaattggctc tttcctgtta
     1261 ggccgcgatc cgaaaaccgg taaacaactg ccatgcccgg ctgaactgct gcgtttaacc
     1321 attccgcgcg caacatatac tcaaacacat atggacttca ttattgaagc ctttaaacat
     1381 gtgaaagaga acgcggcgaa tattaaagga ttaaccttta cctacgaacc aaaagtattg
     1441 cgtcacttca ccgcaaaact gaaagaagtt taattaatac tacagagtgg ctataaggat
     1501 gttagccact ctcttaccct acatcctcaa taacaaaaat agccttcctc taaaggtggc
     1561 atcatgactg atcaagctga aaaaaagcac tctgcatttt ggggtgttat ggttatagca
     1621 ggtacagtaa ttggtggagg tatgtttgct ttacctgttg atcttgccgg tgcctggttt
     1681 ttctggggtg cctttatcct tatcattgcc tggttttcaa tgcttcattc cgggttattg
     1741 ttattagaag caaatttaaa ttatcctgtc ggctccagtt ttaacaccat caccaaagat
     1801 ttaatcggta acacctggaa cattatcagc ggtattaccg ttgccttcgt tctctatatc
     1861 ctcacttatg cctatatctc tgctaatggt gcgatcatta gtgaaacgat atcaatgaat
     1921 ttgggctatc acgctaatcc acgtattgtc gggatctgca cagccatttt cgttgccagc
     1981 gtattgtgga taagttcgtt agccgccagt cgcattacct cattgttcct cgggctgaag
     2041 attatctcct ttgtgatcgt gtttggttct ttcttcttcc aggtcgatta ctccatcctg
     2101 cgcgatgcca ccagcaccac tgcgggaacg tcttacttcc cgtatatctt tatggctttg
     2161 ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc ccagcctgat tatttgctat
     2221 ggaaaacgca aagataagtt aatcaaaagc gtggtatttg gttcgctgct ggcgctggtg
     2281 atttatctct tctggctcta ttgcacgatg gggaatattc cgcgcgaaag ctttaaggcg
     2341 ataatctcct caggcggcaa cgttgattcg ctggtgaaat cgttcctcgg caccaaacag
     2401 cacggcatta tcgagttttg cctgctggtg ttctctaact tagctgttgc cagttcgttc
     2461 tttggtgtca cgctggggtt gttcgattat ctggcggacc tgtttaagat tgataactcc
     2521 cacggcgggc gtttcaaaac cgtgctgtta accttcctgc cacctgcgtt gttgtatctg
     2581 atcttcccga acggctttat ttacgggatc ggcggtgccg ggctgtgcgc caccatctgg
     2641 gcggtcatta ttcccgcagt gcttgcaatc aaagctcgca agaagtttcc caatcagatg
     2701 ttcacggtct ggggcggcaa tcttattccg gcgattgtca ttctctttgg tataaccgtg
     2761 attttgtgct ggttcggcaa cgtctttaac gtgttaccta aatttggcta aatccttcaa
     2821 gaagccagcc attcgctggc ttcttgcctc tcaggaaatc acttatgtcc aaatggcaac
     2881 tcgcctgatt ctccttcacc acgtatgctt tgcgtcacct tactattagg acgctttagc
     2941 ccatgtcccg ctttttaatt tgtagttttg ccctggtttt actttatccc gccgggattg
     3001 atatgtacct cgttggttta ccgcgcatcg ccgacgatct caatgccagc gaagcgcagt
     3061 tgcatattgc gttctccgtt tatctggcgg gcatggcagc tgcgatgtta tttgccggta
     3121 aagtggccga tcgttcaggg agaaagcctg tcgccatccc cggcgcggcg ctatttatta
     3181 tcgcttcggt gttctgttca ctggctgaaa ccagcacgtt atttcttgca ggccgatttc
     3241 tacaggggtt gggcgcaggc tgttgttacg tagtggcgtt cgccattttg cgcgacacgc
     3301 tggatgatcg acgtcgggct aaagtgctgt cattactcaa cggtattacc tgcatcattc
     3361 cggtgttagc gccagtgctc ggacatctga ttatgcttaa attcccgtgg cagagtctgt
     3421 tctgggcgat ggcaatgatg ggcatcgcgg tactgatgtt gtctttgttt attttaaaag
     3481 aaacgcgccc agcgtccccc gctgcttcgg acaaaccacg agaaaatagc gagtcgctgc
     3541 ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca
     3601 ctttcgtcaa tacatcgccg gtattgctga tggaaatcat gggttttgag cgcggagaat
     3661 acgccaccat tatggcgttg actgctggcg tcagcatgac cgtttcattc tccacgccat
     3721 ttgcgctggg aatttttaag ccacgtacgt tgatgatcac ctcgcaggtg ttattccttg
     3781 cagcggggat cacccttgcc gtttcacctt cccatgcggt ttctctgttt ggtatcacgc
     3841 tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat gagtcaggcg ttaggaccat
     3901 tttcattacg cgcgggcgta gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt
     3961 cactgtggat ttggctggca gcggtggttg gtatcggcgc atggaatatg ctgatcggga
     4021 ttctgattgc ctgtagcata gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc
     4081 ccgttgccgc tcatgaagaa atccatcacc acgcttgatc tcaatctgct gctctgtctg
     4141 caactgctga tgcaggagcg cagcgtgacc aaagcggcga agcggatgaa cgtgacccct
     4201 tcagcggtga gtaagtcgct ggcaaagtta agagcgtggt ttgacgaccc gctctttgtg
     4261 aactcaccgc tgggcctgtc gcccacaccg ctaatggtca gcatggagca aaatctggcg
     4321 gaatggatgc aaatgagcaa tctgctgctg gataaaccgc accaccagac accgcgcggc
     4381 ctgaagtttg agttggcggc ggaatcgccg ctgatgatga tcatgcttaa tgcgctgtcg
     4441 aaacggatct accaacgtta cccgcaggcg accatcaaac tacgtaactg ggattacgat
     4501 tccttagatg ccattactcg tggtgaagtg gatattggtt tttccggtcg cgaaagccat
     4561 cctcgctcgc gggagctgtt aagctcgcta ccgttagcca ttgattatga agtgctgttt
     4621 agtgatgtac cctgcgtctg gttacgcaaa gatcatccgg cactgcatga aacgtggaat
     4681 ctggacacct ttttacgtta tccgcatatc agcatttgct gggaacagag cgacacctgg
     4741 gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
     4801 gaatttgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
     4861 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
     4921 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
     4981 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
     5041 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
     5101 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
     5161 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
     5221 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
     5281 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtctagaggt
     5341 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
     5401 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
     5461 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggtct cgatatggaa
     5521 ctacagcgtg cgacgcgcgg gtttcacagc ccacacgcgc ccgataacca caccttttcc
     5581 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
     5641 atcacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
     5701 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     5761 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cacgattgaa
     5821 aagctcagtg tctgggagtt cgatggcaaa cacggctgga aaagcctgcc ggatattcac
     5881 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     5941 gctttttcgc caaattgata gacaaccaca ggagtcatca tgtctgaaaa attgcaggtg
     6001 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     6061 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
     6121 ttgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
     6181 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     6241 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
     6301 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     6361 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
     6421 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
     6481 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     6541 atctaaataa aaaacccgcc agcaatcatg catggcgggt ttttaacgcg ctatcgattt
     6601 tagtgagcgt cgataaagac aatcttcagg ataaacagca gcgcaacgat gattacgcac
     6661 gggcttagat cacgcagacg tccggtaccg attttcatca cgcagtagga gataaagccc
     6721 agcgcgatac cttcggtaat cgagaagctg aacggcatca tcacggcggt aataaacgcc
     6781 ggaacagatt cagtaagatc ctgccagttc acgcgtgcca gactggaagt catcagcacg
     6841 ccaacgtaaa tcagcgcacc agctgcagcg tagcctggca ccatccccgc cagcggcgac
     6901 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
     6961 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
     7021 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaattta
     7081 cccttctcat ccgccaggcc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
     7141 aacaggttga ccagcatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
     7201 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
     7261 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
     7321 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga agaagccgag gatccccagc
     7381 agcacgctgt gagaagtcag gttaccgatg ctgaccagcg tttccgggtt agcgacaatc
     7441 acacctgcat tcttcagtcc catcatacca atgaacagac cgataccgct ggtgatgccc
     7501 acacgcagac ttaccggaat gttggcaatc atccagtagc gaacgcggaa aatcgtcagt
     7561 aacagcagac caatcgcacc ccagaagatt gcgcccatcc cgacttgcca cggcaagccc
     7621 atcgcctgta caacgacaaa agcgaagaac gcattcaggc ccatagcggg tgccagtgca
     7681 actggcaggt tagcaaacag tcccatcata atactgccga atgcagcgat cagacaggta
     7741 gtgacgaaga cggcgctggt atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa
     7801 acgatgtaaa ccatcgtcag gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc
     7861 gtgccatgtt cgcgcagttt aaacacgcgt tccagcatcc cctggccaga agcctgggtt
     7921 gtgtgttgtt gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
     7981 accaaaatga gacaatatgg acggctgaag ataacttttt ttcattgtat ttgcttatac
     8041 ggcaacgatt gcgtcatgcg aattggcatg acgaaaacgt taaactaacg ataaaggaaa
     8101 gacatgtccc agatagaagc ggtatttttc gactgcgacg gtacgctggt cgacagtgaa
     8161 gtcatttgct cccgcgcata tgtaacgatg tttcaggaat ttggtattac gctcgatcct
     8221 gaagaggtat tcaaacgttt caaaggtgta aaactgtacg aaattatcga tattgtttcc
     8281 cttgaacatg gtgttacgtt agcgaaaaca gaagctgaac acgtttaccg tgcagaagtc
     8341 gctcggctgt tcgattcaga actggaagcc atcgaagggg ctggagcgct cctgtcagcg
     8401 atcactgcgc caatgtgtgt ggtatctaac ggcccaaata acaaaatgca gcattctatg
     8461 ggcaagctga atatgttgca ctacttcccg gataaactgt tcagcggcta cgatattcag
     8521 cgctggaagc cagacccggc gttaatgttc catgcggcaa aagcgatgaa tgtaaatgta
     8581 gaaaactgca ttctggttga tgactcagtt gccggtgcgc aatctggtat cgacgcaggt
     8641 atggaagtgt tctacttctg cgccgacccg cacaataagc cgatcgttca cccgaaagtc
     8701 accaccttta cccatctttc gcagttaccg gaactgtgga aagcgcgtgg ttgggatatt
     8761 acggcatagt tcttcacact cccttcactt accccgctta aattggcgct caaaggtaag
     8821 taaagggagt ttgatatgtc tgtttcacgt cgggtaatac atcacggact ttattttgcg
     8881 gttttaggac cgttaattgg tgttctgttt cttgtcctct acatattctt cgcaaaagaa
     8941 ccgctggttc ttttgcgaag aatacaggta cttccattgt ttctcttatt gtcgattact
     9001 acgggagtta ttcctgcatt gttaaccggc gtgatggtcg cctgtctgcc ggaaaagatc
     9061 gggtcacaga aaaattatcg ttgtctggct ggtggcatag gtggcgtcgt tatcaccgag
     9121 atctattgtg cagttattgt acatattaag ggcatggctt cctcggagtt gtttgaaaac
     9181 attctttctg gtgacagtct cgttgtccgc atcattcctg cattgctggc aggtgtggtg
     9241 atgagcagaa tcattacccg tctgcccgga ttggatattt catgtcctga aacagactct
     9301 ttaagttaag cgggatactt tatctttggg ctactcaaaa gcagacagga tgttactatg
     9361 actcaaaata tcaggccgtt accccaattc aaatatcatc ccaagccact ggaaacaggc
     9421 gcatttgaac aggataaaac cgtagagtgc gattgctgtg aacaacagac gtcagtttat
     9481 tactcgggtc ccttttattg cgttgatgaa gttgaacatc tctgtccgtg gtgtattgcg
     9541 gacggttctg ctgctgaaaa gtttgcaggt agttttcagg atgatgccag catagaaggt
     9601 gttgaatttg agtatgatga agaggacgaa tttgccggta ttaagaacac atatcctgat
     9661 gaaatgctga aagagttggt tgaacgcacg ccaggttatc atggatggca acaggaattc
     9721 tggcttgcgc attgtggcga tttctgtgct tttatcggct atgtgggctg gaatgatata
     9781 aaagatcgcc tcgatgaatt tgccaacctt gaagaagatt gtgagaattt cggtattaga
     9841 aattctgatc tagctaaatg cctgcaaaag ggtggtgatt gtcagggtta tctcttccgc
     9901 tgtctccact gcggcaagct gagactgtgg ggtgattttt cgtagttatt taaataatga
     9961 gaacaggccg gagcgtaatt catacatccg gccttatttc ttcagcttaa ccgagcgcta
    10021 attctgcggc agcggcttta tcagcgatta ccatcagcga aggatgcagc tgcaacacgg
    10081 aagctggaac atcttcggta accggacctt gcagaacatt tttcagtgcc tgcgctttac
    10141 ctgcgccact gacaattatc agcaagtttt tcgccgccat gatgcttttc ggccccatcg
    10201 tcacgtagct gtcaggcact aacgaaaagt cgccgcccaa ctcaccatgc gccacaatat
    10261 caaccatctc tccctggatc gggaactcca cggtttgctc atggaaatgg gtcgtattcg
    10321 gcaaattgcc acaaaaatga ccatctgcac ctaatcccag caccaccaaa tccaggccgc
    10381 cttcacgcgc cagtttctga tcatgctcgc ggtagttatc aatagtgagc ttctggatat
    10441 tctcttcttt gatccccgca ggggtgaaaa acagattacg cagattggta atcgttacac
    10501 cttctccctc tttgccgcga aagggaattt catcgaaatt atagaaatag cagttatcgt
    10561 accagggctt acctttaacc agggtggtga ggtattcata catgcctttg ggcgtgctac
    10621 cggcggtaat tgccaggtta acacgacgcg tcttcgacat ataaccaagc agatggtgtg
    10681 ccgcgacacg gctcatttcc tggtaatctt cggtaatgat taatttcatc ttagtgcctt
    10741 attttattta aataatttct gtacgaaggc tgcataggca ggccgccaga catccatttc
    10801 atggttcagg ccgggatatt cctggtaatc aaacttaatt tttttctgct caagctcagt
    10861 tttcagcccg gcgatatcct tgccggttac gacatcttta tcaccaacga ccacagtaaa
    10921 attacgtagt tgctggttaa tagctgccgg atcgttcagc cgggccgcga caccttcatc
    10981 cggtacggtt gtcgtggtaa caccactgaa tgtggccagc cagccaaagc tttccagatg
    11041 attcattccc gataccagcg cctgataccc accttgtgaa agccctgcca gtgcgcgacc
    11101 atcggcatct ttactgacgt taaaacgctt gctaatcagc gggataatat cgttcatcag
    11161 ttcgcgatct gccgctttag cattcagcgg ataaaagact ttacgtcttt cctgaggcac
    11221 gaaatcttcg ggaataatgc ccttcgcatc ggtttcggta tccgggatca ccaccagcat
    11281 cggtttaatt ttcccttcag ccagcaggtt atccatgatt tgcgggatac gcccctgatc
    11341 gatagcggaa cgtccggtat caccaaaacc gtgatagaaa tagagcactg gcaaaggctc
    11401 gcccatgccg gtgtatcctg gcggggtcca gacatacatc tgacgttcag attgcaatgc
    11461 gttggagtgg taagttatgg cgatcaaatc accatgcgca acagaacgcg tatccagata
    11521 actgcctggc accagaatca tactggagtt aacctggcgc tgaggcttag tcattgcggt
    11581 gcctgtatca atgctgcgta caccatcaac attgaaaaaa tactcgtaca ggttgccttt
    11641 caggataggt gtgcgccacg accagactcc tgcttcgtct ttggtcatcg ggtgaatatt
    11701 gtccagaacc ggaacaccca ccactacaga gacatttttt gccccagggg caaagtagcg
    11761 aaaggtcacg ctgttatcgg cattgacctg agtcacatac tgtttcaccg ggatagtggg
    11821 tgccgggctg gctggcatat ccgctgctat ggcccactga gcagaaatac cgctggcaat
    11881 agccagcgtt aaagcagcaa tttttatatt catcgtaata ttcctttcat taagttccat
    11941 taccaccaga tttcagcctg ggcaccgaca gcaaactggt cgtctttatt gtcttcgaag
    12001 gtgaagccat ccagttcgtt ttccagggct ttgatatacg tggcgtagaa acgaatttcc
    12061 ggacgcgagg tcaacatact ggtattgact ttaaaggtat ggaagagcgt ggttttataa
    12121 ccagactcat taaatttatt actattcgca tctttgtttt gctgggtgaa atagcccagt
    12181 tcaacacctg tctggttata ttggtcccaa atataggccg ggcgaacaac cgcacgaata
    12241 gattcgaaat cagagtgtgc gcctgtttcg tagctataaa tatcgttacc gaaggagtaa
    12301 acaatcgcgt tagccacaat gaaatgatcg ccgatatagg cttcgccctg tgaagtcaga
    12361 cgaactgctg ttccgccggt gtgatcacca taataacgac cattaaaggt ggtaaatgga
    12421 cttgcgccag cataacggcc aaagttactg gcgatagagt tattcgcaac caggaaggag
    12481 aattcgttga agccaccttt atcaaatttc tgcgttaaag atgtgccaaa catccaggta
    12541 tctttccagt cataatagcc gttattatcc tgattatctt tttccgatgc gctttcgttt
    12601 gccgtgacat aacgaccact caccattaag gtcgctttat cccataacgg gatatcttta
    12661 tagcgtaaat caatagcatt ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa
    12721 tcatcgacat cttcgcgaac cagcgcgata tcaattttac ccggaccgac tttccagttt
    12781 tccagaccta cacccgctgc ggcatcagta cgctgcgttt tccagtcaag catctggatt
    12841 tcaattttcg gcgcaccgtg tttacccacc cagaaatcag cctctggcgc aaagggcagg
    12901 aaacctttgg tggtaacgta catatcggag aactgcataa agttctcgcc accggcgtta
    12961 tcgccaaacc agccggtaga gtactgctga ccaacgttac catccatcat cacaacggca
    13021 tcaacccgtt tgccgttttc gttgtagaca cgttgtttta actgcaaatc aaaccagccg
    13081 gagtattcgt taccaaagcg gcccagagaa ccaatcgccc atgatttagg tgaaccatga
    13141 gaggcggtcc cccaaccaga acggtaatag ccgttgtaac taaagccaat ttcatctttc
    13201 acaaacttac tgaaatcttt cagagtcatg gaagaataaa tcgagcctgt ctcactggca
    13261 tttttctctt catttttgac cagtacagca tcaggttttg cggcactact ggtcgggaac
    13321 ggattggcgg catacccttg atcattcgta cttacgctac gattcaccgt cgctggcgta
    13381 tattttttct tctcttcatc tttatacttt ttgagttcgc tttgcgtttc tcttaacgct
    13441 ttttctaata actcaaggcg atgttccacc gttaatgatt cagccaatga ttgtgcagaa
    13501 aaggctaacg gtgccattaa taagatggca gaggtaataa gatttcgtct aaacataata
    13561 tccctttatg gtgcaaagaa agaattaacg tatcgcatcc agactgttct gaatgcgacg
    13621 ataattaagg tgctttattg gttatttttt taatgacagc ccccgcgttt tgctcacttc
    13681 tgcataccat ccgaagcttt tcttacgcgt tcttgtgagg cttccttcgc cattatcatc
    13741 acgatccaca taaataaagc cgtagcgctt agacatttgt gaatgagatg cactgactaa
    13801 atcaattggc ccccaactgg tgtaccccat aatatccaca ccatcggcaa tcgcttcatt
    13861 tacctgtacc aggtgatcgt ttaaataggc aattcgataa tcgtcctgta tcgaaccatc
    13921 cgcttcaacg ctgtcttttg cgcctaatcc gttctcgaca ataaataacg gtttttgata
    13981 acgatcccaa agcgtattta acagaacccg taatccaacc ggatcaattt gccaccccca
    14041 ctctgaactt ttcagatgcg gattggggat catattcagt atgttgccct gcgcattttt
    14101 attaatgctt tcgtcgtggg aaacacaacc agtcatgtaa taactaaaag agatgaaatc
    14161 tacggtatgt tttaaatctt ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc
    14221 gcggaagaaa cgctgcatat agccgggata ctgaccacgc gcctgaacat caccaaagaa
    14281 catccagcgc cggttctctt ccatggcctc caacatatcc tgtggctggc aggtgagggg
    14341 gtaaaccagc ccgccgagaa gcatattgcc gattttcgct tcggggatca ggctatgaca
    14401 ggctttaact gcccgcgcac tggcaaccag ttgatgatgg atcgcctgat aaacttccgc
    14461 ctcgccactc tcttctgcca gccccacgcc cgtgaatggc gcgtgtaacg acatattgat
    14521 ttcattaaac gtcagccata acgctacttt atgttggtag cgggtaaaga ccgtgcgggc
    14581 gtaatgctcg aagtgatcga tgaccgcccg attagcccaa ccgccgtagt ttttcaccag
    14641 cccatatggc atttcgtaat gggataacgt taccagcggc ttgatccccg cctgcgccat
    14701 ttcatcaaac agccgatcgt aaaacgctaa ccccgcttca ttcggttcgg cttcgtcgcc
    14761 ctgagggaaa attcgcgccc aggcaatgga aatacgcaga caggtgaagc ccatctcggc
    14821 aaataacgcg atatcttccg ggtaacggtg ataaaaatcg atggcgacat ctttgatatt
    14881 ctctttcccc aggatgcgcg gttccatttt tcccattacg ccatgaggct gtaaatctga
    14941 ggtcgtgatc cctttgccat cttccagcca ggcaccttcc acctgattgg cagttgttgc
    15001 gccaccccaa agaaatgttt ctggaaatgc tttcataatt aactcctttt atcgttagcg
    15061 aatgatggat aacagcggct caccagcgct tatctgcgcc gtgccgtggg gtaatacgtc
    15121 tgtaaaatca tcgctattac tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg
    15181 aatagcaggg atatcaaaag aaatcagccg atcgcctgta ttgaccttgt cacccacgtt
    15241 gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg tcgataccga catgaatcag
    15301 gatctccaca ccatcatctg actcaatgcc aatagcgtgt aatgtggcga acaacgaagc
    15361 aattcgaccc gcaaccggag aacgcacttc accaaccgag ggcagaatgg cgataccttt
    15421 acccaacagg ccactggcaa acgtggtatc agcgacgtga atgagcggca caatctctcc
    15481 cgtcatcggt gaacagatac cgccctgctc aggtggtgta ataacctctg gtgttttctc
    15541 ttgcggggca ccctgcgctg gctgacgttt agcggtgatg aaatgaagca tcaccgtacc
    15601 gacaaatgcg caaccgatgg caatgacacc gccaataacg ctggcccaga cggtgaaatc
    15661 aatccccgtt gacgggatgg tttgcatgaa ggtgaaaata cttggcaaac caaaggagta
    15721 aactttcgtt tgcgcgtagc caatgatggt ggcccccaat gctccactga tacaggcgat
    15781 aacgaaggga tacttacgcg gcaggttgac gccatatacc gctggttcgg tgataccaaa
    15841 caggcccgtc aacgccgctg atcccgccac cacttttttc tgcgcatcgc gttcgcagag
    15901 gaagacgccg agcgccgccc cgacttgcgc cataatggcg ggcattaaca gcgggatcat
    15961 ggtgtcgtag cccagcacgg tgaagttatt gatacacagc ggcaccaggc cccagtgcag
    16021 tccgaacatg acgaagattt gccagaaacc gcccattacc gcgcccgcaa atgcaggaac
    16081 cgcctgataa agccagagat aaccggcggc aatcatttcg cttatccagg ttgatagcgg
    16141 ccccaccagc aaaaaggtga cgggtgtgat aaccatcaga catagcaatg gtgtgaagaa
    16201 atttttgatt gccgacggta accacgcatt aagtcggcgt tccagaatgc tgcacaacca
    16261 ggcagaaaaa ataatgggaa taaccgatga cgagtaattc aacaatgtga ccggaatacc
    16321 caggaaatcc agccccagct catccgcttt ttgcccgttc tcgaaagcag tcagaattaa
    16381 tggatgcact aacgctccac caatcaccat ggcagtaaat ggattaccgc cgaagcgttt
    16441 ccccgcggtg tatcccagga ttatcgggaa gaaccaaaac aaggcatcac tggcgctgaa
    16501 taaaattaaa taagtaccac tttgttcggt cgtccactga aaagtgagcg ccagagccag
    16561 catacctttc aggatcccgg ttgccgccat caaaccgatc agaggcgtaa aaatacctga
    16621 aataacataa acaaagcggt ttagcagatt acctttatca tcattttccg gtgcctgttg
    16681 cgctttttcg tcaaggcctg ccacactgtt aaccgccagg aagacatcgg ccacatggtt
    16741 acctatgacc acctgaaact ggccaccgct ttccaccacc ataataatac cgggggtctt
    16801 tttcagtacc tctgcttgcg ctttgctttc atcctttaac ttaaaacgta atcgcgttgc
    16861 gcaatgcatc agactaacaa tgttatctgc gcccccgact cctgcgacta tttttctggc
    16921 taactccgtc ataacttgcc ctctaccgct ttgcggcaaa actcaaaaaa aaaacctgaa
    16981 aaaaacggcc tgacgtgaat caagcaattt tttcaggttt tgcccgctta gtgcggtaac
    17041 aatcctttac tcagtaataa tatttcagtg ttctttgcgc acgcgctcta tatttatggc
    17101 taaaaacata atctctgcgg gtgagatttt acgttgatac tgcaaaccaa taaaaatggc
    17161 gatccgctcc gcacattgcc atgcttgcgg gtaattttgt tttactgctt gttgtaatga
    17221 ctcatcacta tcgttaattg aagcatgtcc aagaatacgc caggataaaa acttcagatg
    17281 cgtaaccagt cgctgataac tcaagctttc ttcctggtaa ttaaggctga actgaaattt
    17341 tattaattgc agcatttcgc gcattaattg cgtgacacct gcaacatcct ccatatttcc
    17401 gctcatttgg gcactgacca gatgcatggc aataaagccc acttcatctt tcggtaactg
    17461 cacgcccaac cgtttatcaa taatggttaa cgcttcttcc cctagctgga actctttcgg
    17521 gtaaagccgc tggatatccc acagcagcgg gttgggcagt aacacgtttt gctgaaagcg
    17581 tttaatcgca aactggcaat ggtcagttag cgagatataa atactgtcct gtaattttcc
    17641 caagcgctcc tgcgctaaag agataatacg atcacaggtt gccatcacct caagaggaat
    17701 atgacttaag agttcgctta atcgcccgtt cagttcatga ctgctcaagg catactcttt
    17761 ttctattcca cttgagttaa ttctttcgcc agcgcgtttt tgaaagccaa ttccgcgccc
    17821 catgacgact ttttcccgct gttgatcatc aataaccacc acaacattat tgttgagaat
    17881 tttggtgatt tgcatgttca tagcaaggac cttttttata aacaaaaaaa cccgacttca
    17941 ccagtattct ctggttatgt caggttttgc ctgcga
//
LOCUS       DAZFWP020000102        17956 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFWP020000102 DAZFWP020000000
VERSION     DAZFWP020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.102"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            15..722
                     /locus_tag="ACHK0Z_RS16555"
                     /old_locus_tag="pgaptmp_003311"
     CDS             15..722
                     /locus_tag="ACHK0Z_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4503278.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEDSLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            719..2170
                     /gene="djlC"
                     /locus_tag="ACHK0Z_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             719..2170
                     /gene="djlC"
                     /locus_tag="ACHK0Z_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGU4503279.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKSHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2230..3900)
                     /gene="hscC"
                     /locus_tag="ACHK0Z_RS16565"
                     /old_locus_tag="pgaptmp_003313"
     CDS             complement(2230..3900)
                     /gene="hscC"
                     /locus_tag="ACHK0Z_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU4503280.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3984..4919)
                     /gene="rihA"
                     /locus_tag="ACHK0Z_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             complement(3984..4919)
                     /gene="rihA"
                     /locus_tag="ACHK0Z_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU4503281.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5037..5762)
                     /gene="gltL"
                     /locus_tag="ACHK0Z_RS16575"
                     /old_locus_tag="pgaptmp_003315"
     CDS             complement(5037..5762)
                     /gene="gltL"
                     /locus_tag="ACHK0Z_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU4503282.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5762..6436)
                     /gene="gltK"
                     /locus_tag="ACHK0Z_RS16580"
                     /old_locus_tag="pgaptmp_003316"
     CDS             complement(5762..6436)
                     /gene="gltK"
                     /locus_tag="ACHK0Z_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU4503283.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6436..7176)
                     /gene="gltJ"
                     /locus_tag="ACHK0Z_RS16585"
                     /old_locus_tag="pgaptmp_003317"
     CDS             complement(6436..7176)
                     /gene="gltJ"
                     /locus_tag="ACHK0Z_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU4503284.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7346..8254)
                     /gene="gltI"
                     /locus_tag="ACHK0Z_RS16590"
                     /old_locus_tag="pgaptmp_003318"
     CDS             complement(7346..8254)
                     /gene="gltI"
                     /locus_tag="ACHK0Z_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU4503285.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8709..10586
                     /locus_tag="ACHK0Z_RS16595"
                     /old_locus_tag="pgaptmp_003319"
     CDS             8709..10586
                     /locus_tag="ACHK0Z_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGU4503286.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10664..12202)
                     /gene="lnt"
                     /locus_tag="ACHK0Z_RS16600"
                     /old_locus_tag="pgaptmp_003320"
     CDS             complement(10664..12202)
                     /gene="lnt"
                     /locus_tag="ACHK0Z_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU4503287.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYKSADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12227..13105)
                     /gene="corC"
                     /locus_tag="ACHK0Z_RS16605"
                     /old_locus_tag="pgaptmp_003321"
     CDS             complement(12227..13105)
                     /gene="corC"
                     /locus_tag="ACHK0Z_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU4503288.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13195..13662)
                     /gene="ybeY"
                     /locus_tag="ACHK0Z_RS16610"
                     /old_locus_tag="pgaptmp_003322"
     CDS             complement(13195..13662)
                     /gene="ybeY"
                     /locus_tag="ACHK0Z_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU4503289.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13659..14699)
                     /locus_tag="ACHK0Z_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             complement(13659..14699)
                     /locus_tag="ACHK0Z_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU4503290.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14852..16276)
                     /gene="miaB"
                     /locus_tag="ACHK0Z_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             complement(14852..16276)
                     /gene="miaB"
                     /locus_tag="ACHK0Z_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU4503291.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16422..17597
                     /gene="ubiF"
                     /locus_tag="ACHK0Z_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             16422..17597
                     /gene="ubiF"
                     /locus_tag="ACHK0Z_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU4503292.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17751..17825)
                     /locus_tag="ACHK0Z_RS16630"
                     /old_locus_tag="pgaptmp_003326"
     tRNA            complement(17751..17825)
                     /locus_tag="ACHK0Z_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17791..17793),aa:Gln,seq:ctg)
     gene            complement(17874..17950)
                     /locus_tag="ACHK0Z_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     tRNA            complement(17874..17950)
                     /locus_tag="ACHK0Z_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17914..17916),aa:Met,seq:cat)
BASE COUNT         4378 a         4516 c         4671 g         4391 t
ORIGIN      
        1 gggattatta acaaatgaat aaggaagaac aatatcttct ttttgcgctt tctgcgccca
       61 tggaaatact taatcagggt tgcaaacccg cgcatgactc gccaaaaatg tataccggta
      121 taaaagaatt cgatctctca tcatcctggg gaataaataa tcgtgatgat ttaattcaga
      181 ctatttatca gatgactgat gatggtcatg ccaatgattt agctggctta tatctgacat
      241 ggcatcgctc ttcccctgaa gagtggaaag cattaattgc tggtggctcg gagagagggt
      301 tgatttatac ccaattcgtt gcccaaaccg ctatgtgctg tggagaaggg ggaatcaaag
      361 cctgggatta tgttcgaatg gggtttctgt cacgggtcgg tgtgctcaac aactggctga
      421 cggaagaaga tagtttatgg ctgcaatcgc gcgtctatgt acgagctcac cattactatc
      481 acagctggat gcactatttt tccgcctatt cgttaggacg tctctactgg caatcttctc
      541 agtgcgaaga caacacttcg ctacgcgagg cgctaaccct gtacaaatac gatagcgccg
      601 ggagtcggat gttcgaagag ctggcagcgg gaagcgatcg tttctatgcc acgcttccct
      661 ggcagccatt aactgtccag cctgaatgcc cggtaacgct taaggatgtg agcgacctat
      721 gaaaacatgt tggcaaatcc tcgaaattga aagcacgacg caaatagaca ttatccgcca
      781 ggcttatctt gctcgcttac cgttatgtca tcccgaaacc gatccgcagg ggtttaaagc
      841 attacgccag gcctatgaag aggcgctgcg actggcggta aatcctgtcg aggaagcaga
      901 tgatgaagaa aaagatgccg ccgctgaaca tgaaatactg cgtgcattca ggacattact
      961 ggattcagaa agtgatcgtt ttcagccctc cgcctggcag aaatttattc agcaattaaa
     1021 tacctggaac atggaggatg tcgatcaatt acgctggccg ctgtgtgcaa tcgctataga
     1081 agcgcgatat ctttcattaa attgtgcttc tttgctggca gagcgtttga actggcattc
     1141 gtttaatgac agcgaaggaa tggatgagga agaaagggag gcttttcttg aggccattca
     1201 ggctggtgat tgtttcgatt tccttagcct tctggaatat cccgttgcgt tgcagaacca
     1261 gactgttgag tattacttcg cgctggaacg ttgctgccgt taccatcctg actacgtcac
     1321 tgcgtttttg gcgatggaag gtccgtggtt cattcctgat gatgcaaagt tacatcgcaa
     1381 actgttgcgc tggtacagct cggtgcaaac aggtatggcg gaactcattc ctgtagccaa
     1441 acagtggcaa atggaagaac cagaaagcga agatgcccga tattacttgt gtgctcaaag
     1501 gttgtactgc ggcgaggggg aaagcctgct tgccgatctc tgcgcgtacc gggaaagtta
     1561 cccatctaca caagctgata atttgttgtt gcagtggagc aagagtcatt gcccggatta
     1621 tttcgcgtta ttagtgatgg ttatcgaagc gcggagcatg gtagatgcgc aaggtcaacc
     1681 gctgaaatat gttcctggtg agagcgcccg gacgcggctg ttatgggcgg aaattttaca
     1741 tagcggaaaa ttatcgccgt taggtcaatc gtttattgag tcgttattct tcaaacgcaa
     1801 agcatgggcg tggtggaaat cgagagtcgg tagcgagaca gaagaagatt caccgttact
     1861 ggatttgtat cgggtagcgg aacaggtagt acttgaagcg tttccgaaac aagagatgct
     1921 ggcccgtctt aatacaaggc tggaaggcgg agatgctcat ccattagagg ccattatcac
     1981 ccggatgctg ttgacgaaag taaaactcga gccggaggat gaagatgtcg atgagccaac
     2041 acctgaaaac catgaagaaa aaaatgatga gggtgaaaaa ccacagagca ttaccagcat
     2101 tatcaaaatc agtttaacgg tgctggttat aggttatgct ctcggcaaaa tcgcgatgtt
     2161 gtttagttga cgatgtgttt tttgcgccga tgccggatgc agcatttacg ccgcatccgg
     2221 cagccgtgat tatgaggtct caatggctaa ataaacgctc gcccgccgcc tgacctcatc
     2281 gacgcgggcc atatcgtttg actgcaacgc cgcatcaaaa tcggtgatca cccggccaat
     2341 ctcttctcgc tcgtcaccca gcgcccgcgc ccacaactct tccagtttgg ccttaaaggt
     2401 gcggttgatg agcatatcgc gcggataaat tttcaatgcg gataaccgcg tccgactctc
     2461 ttcaatctgc tgcgcgctca atgttaccgg gctatgatta atgactctgg acttaacgct
     2521 gccatcttcc agaagcacgt caacttcaag caagccgttg atatcataac taaagcgaat
     2581 atcaatcgac tgataagccc cggttttctt caacggcaca tcgaaggatt caaccagaat
     2641 gttattttta actttgtggt tttctccctg atagacgtta accgtaattg aatcctgttc
     2701 cgggtgcatg gtcgagtaag tttctacacg cgacacgggt actgtggtgt ttcgttcaat
     2761 aattggcgag aaaatgccgg aaacgccctg gcggttaact tcaacgccca acgagtaagg
     2821 gcaaatatca gtgaggatca cctcttcaat atcttcactg cgtaagcggc aggcggcctg
     2881 gattgctgcg cccagcgcga caatggtgct cgggtcgtaa ctttgatacg gtaatttgcc
     2941 aaacagacgc acggcgattc gctgcaccag cggcatttgt gacgcgccgc caaccagcac
     3001 cagactgtcg atttgactcg gcttcagacg tgcatcgcgc aacgcctgtt caatcggcac
     3061 tcgcaagcga ttgagcagcg gcaaccacaa atcttccagt tcgttctcgt aaaattcgca
     3121 ctcccgcgtt tcatcctggt actgccagcg aatgtgcaat ggcgattgat tgctacattt
     3181 tgccgcttcc acacaggcgt acaaggctgc cagttcactc tcgttaagcg tggtcctggc
     3241 gacatccgcg cgctttaaaa cgtcatcgac caacatatgg gtaaaatctt cgccaccgag
     3301 gaagttatcg ccagcggagg cgtgaacttc aatcaccggc gtggcgtatt cgagcaccgt
     3361 gacgtcaaac gtgccgccac cgagatcaaa aaccagcgag cgggtatttt gttgcgtatg
     3421 caggccatat gccatcgcag ctgctgtggg ttcattaatt aagcgtaccg catttaaccc
     3481 ggctaactcc gctgctaaac gggtatgctt gcgttgttca tcgctgaaat aagccggaac
     3541 ggagatcacc acatctttaa tcggacgttg cagaaattct tcggcatctt cttttaatga
     3601 gcgtaatacc aaagaggaca gttctggcgc gttaaatgtg tcgctgccta accgccagtt
     3661 ggtattactg cccattgcac gtttaaataa cgctgccgtt ttatccggat gcgaagtacg
     3721 ccgtgataca gccggttttc cgactaaaat atgattattt tcatccatgc taattatgga
     3781 tggtgttaaa tattcaccga acttatttgg aattaattgc gcggcaccgt ctttccagac
     3841 ggcaattaaa ctattggtag tgccgagatc gataccaatg gcgagttctg cattatccat
     3901 tgcatttata tcttgaaaga aaaagagata aacagattaa gaccacccag gtaacgcgtg
     3961 gtgatcttaa tcaatgacgt gtgttaagcg taaaatttca gacgatcagc cagtaaatca
     4021 acaaagccct gacgatcaac atcgaccatt acagtggcat tcggtttatt gccagtcaga
     4081 taataataat caacaaccgt cataccctgg ctatatttcc cctgtgtttc cacgccaacc
     4141 cagcgctcaa cagtggtaaa caactcgggt ttcaacagcc aggcgatggt gcatgggtca
     4201 tgcagcggtg caccgacaaa gccccatttt tcgtctttat gatattcgag gaagaaatcc
     4261 agcagttcgg caacaatggt tgaaacaggg ttgccaatcg cgcggaaacg ctcggtgtct
     4321 tcaacgtgga tttgtgcttt atgagtaaca tccagaccgg ccatcaccac cgggatccct
     4381 gactggaaga caatttctgc cgcttccggg tcaacgtaaa tattaaattc agccgcaggc
     4441 gtccagttac caagccccat tgcgccgccc ataatcacga tacgggcaat tttgctatgc
     4501 agttccggat ggctattgag cagcaaggca acgttagttt gcggtccggt agacacaatc
     4561 gtgacaggtt ccgcactttc acgcagcgtt ttcgccatta gctctaccgc cgtacagttt
     4621 tgcggtgcga acgccggttc cggtaatgcc gggccgtcga gaccgctttc gccgtgcaca
     4681 ttgtccgcga taatcaactc acgcattaac ggttttaccg cgccgcctgc taccggaata
     4741 tcggtgcgat tgagcaaggt cagcatacga agaacattgc gtaaggtttt ttctggtgtc
     4801 tggtttccgg cggaagacgt aattgctttg acatcaagct ctggtgaggc gagggcgaga
     4861 actattgcga tagcgtcgtc atgacctggg tcgcaatcta acagaattgg cagtgccatt
     4921 gttgctcctt gttgtgtgct tctttgcgac aagggtaacg ccaggatgta acagatacga
     4981 ggggcgaaac gataaagcgt gagatggcgc gcacttgggt atgcgcgcca gagtgattaa
     5041 tgcaggattt tcgcgaggaa gtcttttgcg cggtccgatt tcggatcatc gaagaaagcg
     5101 tctttcggcg agtcttcgac aattttaccc tcatccataa agatcacccg attcgccact
     5161 ttgcgggcaa agcccatttc gtgggtcacc accatcatgg tcattccttc gttcgccagt
     5221 tccaccatca cgtccagcac ttcgttgatc atctccggat ccagcgccga cgtcggttcg
     5281 tcgaacagca tcgcaatagg atccatacac aatgcgcgag cgatcgccac acgctgctgc
     5341 tgaccgccgg aaagctgcgc cggaaattta ttggcatgtg cggaaagtcc cacgcgctcc
     5401 agcaatttca atgctttttc acgagccggc gctttatcgc gtttaagcac tttcacctgc
     5461 gccagggtca ggttttcgat aatcgacaga tgagggaaca actcaaaatg ctggaacacc
     5521 atcccgacgc gggaacgcag ctttgccaga tcggttttct tgtcattaac cacgatacca
     5581 tcgacggtga tttcaccttg ctgcactggt tcgaggccgt tgacggtttt aatcagcgtt
     5641 gatttgccgg agccagacgg gccgcaaacc accaccactt cgcctttttt cacttcggtt
     5701 gagcagtcgg tcagcacctg aaagtgacca taccattttg aaacattttt cagggtaatc
     5761 attatgctgt ccttcttttc aagtagctga ccaacaacga cgcgctaaga ctaataacga
     5821 aataaacaaa tccggcaaac aggatcatct caacctgcgt accatcacgc tcaccaatgg
     5881 ttgaggcggt acggaagaaa tcggccagag ataacacata caccagtgag gtatcctgga
     5941 acagtacgat gccctgagtg agcagaagcg gcaccatcgc gcggaacgcc tgcggcagaa
     6001 taatcagttt catcgactgc cagtgagtca ttcccaacgc cagcgcggcg ctcgactgac
     6061 cacgagaaat actttgaata ccagcacgga taatctctga atagtaggcc gcttcaaaca
     6121 tcgaaaacgc caccatcgcc gaaattaaac ggatatcatt ttttggcgat aatcccagca
     6181 cgttttgcag aaaacccggc acgatcaggt aaaaccacag caaaaccata actaaaggaa
     6241 tcgagcggaa tacgttaacg taggctttgg caaaccacgc tacgggcgca aagctggaca
     6301 agcgcatcac cgccagcatg gtgccccaca aaataccaat cacgactgcc gtgacggtga
     6361 ttttcagcgt gatcaccagc ccgtcgagca gatatggcag ggaaggaaca atggaactcc
     6421 agtcaaactc gtacattatt tgccccccat gttgccgggc aggcgaactt tacgttcaac
     6481 cagcgtcatc accagcatga taaccgcgtt aatcaataca tacgccagcg taatggcggt
     6541 aaacgactcc caggcgtgag ccgagtagtc gagcaattta cccgcctgcg ctgccatatc
     6601 caccagaccg atagtcgagg cgatggcgga gtttttcacc aggttcatca tctcggaggt
     6661 catcggcggg acgataacgc gataagcatt aggcagcagt acatagcggt aagcctgcgg
     6721 tagcgtcagg cccatcgcca gcgcggcatt cttctgccca cgcgggagcg actgaatagc
     6781 ggcgcgtacc tgttcgcaaa cacgggcggc ggtaaacagc cccaggcaga gcatggatga
     6841 aaggaaaaac tgaatattgg gatccagctc ggccttaaac cacatgccga ttttctccgg
     6901 caacagctcc gggatcacca gataccaggt aaagaattgc acaatcaacg gcacgttgcg
     6961 gaacagttcg acatacaacg tacccagacc ggagagaaaa cggtttggaa cggtacgtaa
     7021 aatgccgaaa aatgaaccga cgaggaaagc gataatccag gcgcagatcg acaaagcgat
     7081 cgtcacctga aaaccactcc agatccaacc aagataggtg gtgttgccga acggggcttg
     7141 ttgtaaaaaa ataccccagt tccagtctat agacataaat ctactccaga aaaaagaggg
     7201 tagcagcgtt aactgctacc ctcgaagatt gttacccagc gtattgcggt tttcaggccc
     7261 gatggggaac gaccatcagg cgtatagtct gtccgtgcta cgtaacaatc gagagggctg
     7321 gaatttccgc ccctggttct tgtaattagt tcagtgcctt gtcattcggt tctttgaaca
     7381 gtgctttcat ttcgtctgac agttcgaaat tcatgttcag gtttttcggc ggaattggat
     7441 ttttgaacca tttatcaaac catttttccg cttcaccgga ggtctgcacc tgagcgatgg
     7501 tgtcatccat cagctttttg aactgcggat catctttacg caacatacaa ccgtaggcct
     7561 cctgagactg cggcttgccg acgatttccc agttgtctgg tttcttcgct ttcgcacgtt
     7621 caccggccag cagggcatca tccatcataa aggcaacggc acgaccgctt tccagggtgc
     7681 ggaaagagtc accgtgatct ttggcgctga tgatgcgcat attcattttt tgctcttcat
     7741 tcagtttgtt gagcaaaact tcagaggtag tgccggaagt gacgactacg gctttgtctt
     7801 tcaggtcggc aaaatctttg atatcgccgc cctttttggt cagcaggcgc gtaccgacca
     7861 cgaaaatagt gtcagagaaa gccgcctgtt tttggcgttc gacgttgttg gtggtagaac
     7921 cacattcaaa atcaaaagtg ccgttttgca gcagcggaat acggttttgt gaggtaatcg
     7981 gaatcagttt tacctgcaag tccggtttgt tgagtttctt tttcactgct tcaacaatgg
     8041 cgttggagta atcctgcgag taacccacca ctttttgctg attgtcgtaa taagagaaag
     8101 gtactgaaga ttcacgatga ccgacgacaa tcacaccgtt tttggcgatt ttgtccagcg
     8161 tactgcccgc taccggggcg gcgtcatctg cctgtgccag tcctgcggaa agcgccaggg
     8221 cgaggattgc tgtggcaggt ttacgtaatt gcatatccaa ctcctttatc ttctgcgtta
     8281 agaacgcatg gatacccgtt gtgagtgttt gtgttgttat cgtctgcaac tttattgtgc
     8341 agtgttgtgc ctgttaggta acatttagtt tggctaaatg taaagatatt gctgttttat
     8401 tgtttgtttt tgcgagatgc gccgcaccat tccgaagcaa aattcttaaa ccgcaccttt
     8461 ttggtgctac cgctgatttg tggtggtgca acctggttgc gtcgcggctg atttcatcgc
     8521 ttacacagta aataaagcaa cttacatgcc aaaaaatact taggggcgaa ttaattacga
     8581 ttgtaatttt aggctgaaaa agtcatattt cggacagaat tatcttttcg gcatgggacg
     8641 tttgccggga tgataactgc acacgggaaa aatgcttttt acctgtcttt tcaaggaaga
     8701 aacaccttat gtctgcatct tcggttaagc cgttaaatgt tcaattaccc gcaataaccc
     8761 ttatcctttt tgcactctgt attgggatat tttgttatct cgcacaatgg atgagttatg
     8821 aagaggtcga tcaatccgca cttatccatc tcggcgcaaa cgttgcgcct cttactttgt
     8881 cgggtgaacc ctggcgctta ttgagcagta tctttctgca cagtagtgtt tctcatctgc
     8941 tgatgaatat gtttgcattc ctggtcgtgg gaggcgtggc ggaacagatt ctggggaaat
     9001 ggcgactcct gattacctgg ttattctccg gcgtctttgg tgggctcatc agcgcctgtt
     9061 atgcgttacg cgaaagtgag cagatagtca tcagcgttgg ggcatccggg gcgattctgg
     9121 gaatagctgg tgcagcgata gcaacacagt ttgcttcagg tacgggcaca taccataaaa
     9181 accagcggcg agtatttcct ctgttgggta tggtggcgct gacactgttg tacggtgccc
     9241 ggcaaacagg aatagataac gcttgccaca ttggcggcct gattgcgggt ggcgcgttgg
     9301 gttggctgag cgcgcgttta tctgggcaaa accgactcgt tacggaaggc gggattattg
     9361 ttgcgggcag tcttcttctg accggggcta tctggcttgc gcagcagcag atggatgagt
     9421 cagttttaca agtcaggcaa agcctgcgtg aagcgtttta tccacaggag attgaacaag
     9481 agcgacggca aaaaaagcag cagttagcgg aggaacgcaa cgccctcagg gaaacattat
     9541 ccgctccggt aagtcgtgaa caggccagtg gtgatttgct cgctgagatt gccgatatcc
     9601 atgatatggc gatcagtcgg gatggtaata cgttgtatgc cgcaattgaa gacaccaaca
     9661 gcattgttgt tttcgacctc ggacaaaaga aaatcctgca tacctttaca gcgcccatag
     9721 cgaaagaaaa gtcagtcaaa cattgtggtg gctgtaaaga tcagggcgtc agatcgctgg
     9781 cattaagccc ggatgaaaag ttgatttatg cgacttcatt tgaagcgaat gcgttatcgg
     9841 tcattaacgt ggcgacaggg gagattattc agtcgattac caccggtgca catcctgaca
     9901 gtcttatcct ctcgcgtgat ggcacaaaag cctgggtgat gaatcgcacc agtaatagtg
     9961 tgtcagccat tgatctggtg gcttatcagc atgtggcgga tatcccgctg gagaaatacg
    10021 acgggacggg gacgagcggt aaaccaggcg cctgggttat ggcactttcc ccggatgaaa
    10081 gaacattact ggttccggga gaaggcagag gtaacatcgt gcggatcaat accatcacgc
    10141 atcagaaaga agactttccc gcaggtgatg cgcgtggaac gatatcggcg atgcgttttc
    10201 gacctgaaaa cggcgaggtt atttttgccg atagtcaggg gatttcacgt ataagcatag
    10261 gggatcaaca aaccagcatt atgacgcaat ggtgtagcag gagcgtttat tccgttgagg
    10321 gtattagccc ggacggtcaa tatttagcgt tggtgtcata tggactgcaa ggttatgtca
    10381 tcctgctcaa tattaatgcc gggcagatta ttggcgttta tcctgccagc tacgttaatc
    10441 accttcgttt ttcagcggat ggtagaaaaa tatttgtcat ggcgaagaac gggttgatcc
    10501 aaatggacag gacgcgctcg cttgatccgc aggcaattat tcgtcatccc caatatggca
    10561 atgtggcttg tatccctgaa ccgtaagaaa aaggggcatg ataaccatgc cccaaagctt
    10621 aatgagaaag tacgttttgc tcatgcagga tacatcaggc acatcaacgt ttacgtgctc
    10681 gcagactcat cagcacagcg gcaaaaccaa acagcgccgt cagcacccac agcggccagt
    10741 tgccggtacg tgcatacggt gtgagtccgg tggtcggtgt cacattagtg gtcaacacct
    10801 cgcgggtgaa ctgcgggatc atcgcctgaa tctcaccctg cgggccaatc accgccgtaa
    10861 tgccgttgtt ggtgctgcgc aacagtgggc gcgccagctc cagcgcacgc attcgcgcca
    10921 tctggaagtg ctgccatgga ccaatagact taccaaacca cgcatcgttg gagatagtca
    10981 gcagatagtc ggtatccggg cggaagttat cgcgcacttg ctcgccgaga atgatctcgt
    11041 agcaaatagc cgcagtaagc tcaataccat ttgccgacag cggcggctgg atatatggcc
    11101 cacggctgaa cgacgacatc ggcagatcaa agaacggtgc taacggacgc agaatcgact
    11161 ccagcgggac aaactcgcca aacggcacca gatggttttt gttatagcga tcggctgatt
    11221 tgtagctgta cggtgcacct ttacccagcg tgatgatggt gttgtaggta tcgtagcggt
    11281 tctgcttatt gagacgcgcg tcgacaatcc cggttaccag cgagctacct ttatcgcgca
    11341 actcaccgtc cagtgctttg aggaacggtt gctgattaat ttccagatcg gttatcgccg
    11401 actccggcca gataatcaac gatgatttgc ccatcagcgg agccgttgcg ttgtagtaaa
    11461 tcttcagcgt attaagaagc tgatctccat cccatttcag tgattgcgga atatcgccct
    11521 gaaccatcga aacctgaatg gttttctccg gttgtggggt aaaccattgg atgtaacgca
    11581 gcgggaaggg tagggcaaac agcacgacgg ccaccaccag cggacgccag ttgcgtttga
    11641 ccaacgccag tgccagcagg ccactaacca tcatcagcag gaagttaatg gcttccacgc
    11701 ccattatcgg tgccagccct tttaacgggc catcaatctg gctatagccg aactgtaacc
    11761 acgggaagcc tgtcagtacc caaccgcgca gaaactcggt cacttgccag agagaagggg
    11821 cggcaatcgc cacgcgcagc caggtggttt tcggccacag acgcgacagc acgccagcaa
    11881 acagtccggt atacagcgac aaatacgccg ccagcagcac caccaggaag atgttaaccg
    11941 ggccaggcat cccgccaaag gtcgcgatgc tgacatagac ccagttaata ccgctgccaa
    12001 agaggccaaa tccccagcaa aagccaatag cggcagactg gagtggacgg cggttaaagg
    12061 tcaacgcctg aagccccatc agcgaaataa tcgccgcagg ccagacatcg taaggagaga
    12121 aggccagcgt tccgcaggca ccgaataata acgccagcag caggcgaatg cgctggcgtt
    12181 caattaatga ggcaaaagcc atgtagttat ctatccagtt tcggttttat tcatccagct
    12241 tcggctgggg tgagtcatcc gggattttga catgaacctg aataatacgc cgactgtcgg
    12301 ccatcgccac tttgaactgg taaccgtcga tgtcgatagt ttcgccacgc gccggaagat
    12361 gcccaaatgc ctgcatcacc agaccaccga tagtgtcaac ctcttcatcg ctaaagtggg
    12421 tgccgaacgc ttcgttgaag tcttcaatgg aagccagtgc gcgcacggtc caggtatgac
    12481 gactcagctg acggaagtcg atatcatctt cttcgtcata ctcgtcttca atctcaccaa
    12541 caattagttc caggatgtct tcaatggtca ccagaccgga aaccccaccg aattcgtcaa
    12601 taacgatcgc catgtggtaa cgctgagagc gaaactcttt cagcatccgg tctacgcgct
    12661 tactttcagg aacgacaacc gcctgacgta acactttgtc catgctgaag gcttcagcat
    12721 cgctgcgcat aaacggcagc aagtctttcg ccatcagaat cccttcaatg tgatctttgt
    12781 cttcgctaat caccgggaaa cgtgagtggg cggactcgat gatgacatca aggcattcgt
    12841 ccagcgtctg gttgcgtttc agggtaatca tctgggagcg ggggatcatg atgtcgcgaa
    12901 cgcgttggtc tgcgatgtcc atcacccctt cgagcatatc gcgcgtatct tcgtcgataa
    12961 ggtcgttctg cccggaatca cggatcagcg ccagcagttc gtcacggttt ttcggttcac
    13021 cgtggaaaag ttggctgagt aacagggaga aaaatccctt cttgttgctt atcgtgtcac
    13081 tactgtgtga attgtcgtcg ctcatggcgt cgtatgggtt ctcatgttag ttaatcaaaa
    13141 cgccgtcgtt aatcaccaac ggcggggacg tctgccagtc aaatgcctgg caaattattc
    13201 tttctcggca atgtacggat cctcatagcc cagagcaagc ataatctctg tttcgagggc
    13261 ttccatttct tctgcttcgt catcttcgat gtgatcgtaa cctaacaaat gcagactgcc
    13321 gtgcaccacc atatgcgccc agtgcgcctc cagtggtttg ccttgctcct gagcttcctt
    13381 ctcaaccacc tgacggcaga taaccagatc gcccagtagc gacatttcca tgccaggcgg
    13441 cacttcaaac gggaaggaga gcacgttggt cggcttatcc ttaccgcgat aggtcagatt
    13501 cagactgtgg ctttcggcgg tatcgaccac gcgaatagtc acttccgatt cttcctgaaa
    13561 ctgcgggatc accgcattca gccatgtttg aaactggctc tcttccggta acccagaatt
    13621 atcttcacat gccagttgta aatcgaggat cacctgactc atttttgttc ctgttcttcg
    13681 cgcttgcgtt ctgccgccag cgccgctttt cgtttttgtt cggcttcttc ccaggcttca
    13741 taggcgttaa cgatacgcgc caccaccggg tgacgaacca cgtcttcgct gtggaagaag
    13801 ttaaagctga tctcttcgac atcggccagc acttcgatgg cgtgacgtaa gcctgattta
    13861 gtattacgcg gcaggtcgat ctgtgtgacg tcgccggtga taaccgcttt tgagttaaaa
    13921 ccgatacggg tcaggaacat cttcatctgt tcgatggtgg tgttctggct ctcatcgaga
    13981 atgataaacg cgtcgttcag cgtacgacca cgcatatagg ccagcggcgc gacttcaata
    14041 acgttgcgct caatcagttt ctcaactttc tcaaagccca gcatttcaaa cagcgcgtcg
    14101 tacagcgggc gcagatacgg gtctactttc tggcttaaat cgccaggcag gaagcccagt
    14161 ttctcaccgg cttcgaccgc cggacgagtc agcagaatac ggcgaatctc ctgacgctcc
    14221 agggcatcaa ctgccgcagc cactgccagg taggttttac ccgtacccgc cgggccaacg
    14281 ccgaaggtaa tgtcatggtc gagaatattg gcgatgtact gcgcctggtt tggcgtgcgc
    14341 ggcttaatta cgccgcgttt ggttttgata ttgaccgctt tgccgtactc cggcacgctc
    14401 tccgcacttt gctccagtac tcgcgcttct ttaatcgcaa ggtggatctg ttccggttcg
    14461 atatcctgaa tctgaccgcg catcggggct gtatcgacat acaggctacg cagaatgtct
    14521 gccgcagcgg tgacgcaaat cggacggccg gtcagtttaa agtggttgtc gcggcgattg
    14581 atctcgatgc cgagacggcg ttcgagctgc ttgatgttgt catcaaacgg gccgcacagg
    14641 ctcaacagac gcgcattgtc tgctggctcc agggtgattt cgcgagtgtc tatgttcaaa
    14701 ccgttcctct tatctgtatg ccgccggaag ctgaacattc accggcctat aaggaaatta
    14761 ttcacgccac aggaaaaagg cgcaagcatt gcgataaaga tggggataaa gagagaaaaa
    14821 acaaggccca ccggaacggc gggcctgaga attacggctg ataataaccc acgccaaggt
    14881 cattttcttt gcgagtacgg gcaatcaccg attcgggtgt ttctgccacg cgcagaccca
    14941 tttcatcttc agtacgcacc actttaccgc gcagagagtt cgggtagacg tcggtaattt
    15001 ctacatcgac gaatttaccg atcatatccg gtgtgccctc gaagttgacc acacggttat
    15061 tttcggtacg cccggaaagc tccatgatgc tcttacgcga tgtaccttct accagaatac
    15121 gctgggtggt gccgagcatc cgacggctcc atgccatcgc ttgctgattg atacgttctt
    15181 gcagaatata cagacgctgc ttcttctctt cttccggaac atcatcaacc atatcagcgg
    15241 caggcgttcc tggacgtgca gagaagataa agctgtagct catgtcgaaa ttgacgtcgg
    15301 caatcagctt catcgttttc tcgaagtctt cggtggtttc gccagggaag ccaacaatga
    15361 agtcagaact gatctgaata tccggacgcg ccgcacgcag tttacggatg atcgctttgt
    15421 actccagtgc cgtatgagta cggcccatca ggttcagaat gcgatcggaa ccgctctgta
    15481 ccggcagatg caggaagctc accagctccg gcgtgtcgcg atacacttcg atgatatcgt
    15541 cggtgaattc gatcggatgg ctggtggtaa agcgaatacg atcgatcccg tcgatcgcag
    15601 caaccagacg caacagatcg gcaaacgatc cggtggtgcc gtcgtagttc tcaccacgcc
    15661 aggcattcac attctgaccg agcaggttga cttcacgcac gccctgagcc gcaagctggg
    15721 cgatctcaaa cagaatatcg tcggacggac ggcttacctc ttcaccacgg gtgtaaggca
    15781 ccacgcagta ggtgcaatat ttattgcagc cttccatgat ggagacaaac gcggtcggcc
    15841 cttcggcgcg cggttccggc agacggtcaa acttctcgat ttccgggaag ctgatatcta
    15901 caaccgggct gcggtcgccg cgcacggagt tgatcatctc cggcagacgg tgcagcgttt
    15961 gcggcccaaa aataatatcg acatagtggg cgcgctggcg aatgtgctcg ccttcttgcg
    16021 atgccacgca gccaccgacg ccgataatca ggtctggatt cttctctttt aacagtttcc
    16081 agcgacccaa ctgatggaag actttttcct gagccttctc gcggattgag caggtgttca
    16141 gcagcagcac atccgcttct tccgccacgt cggtcagttg atagccgtgg gtggcatcca
    16201 gcagatcggc catcttcgat gaatcgtact cgttcatctg acagccccag gttttaatat
    16261 ggagtttttt ggtcatcgac ttgctcttgc gaaatagtag ccaggaatgc agggcgcata
    16321 gtgtaatgct ttgctgccgt tgtgaccagt atgagcgtta tcagcccttg ggggtaaaaa
    16381 tcctgtaaac ttaaagcata ttgctaacag gatgattgac catgacaaat caaccaacgg
    16441 aaattgccat tgtcggcgga ggaatggtcg gcggcgcact ggcgctgggg ctggcacagc
    16501 acggattttc ggtaacggtg atcgagcacg cagaacctgc accgtttgtc gctgatagcc
    16561 aaccggacgt gcggatctcg gcgatcagcg ccgcatcggt atcattgctt aaagggttag
    16621 gggtctggga tgcagtacag gctatgcgtt gccatcctta ccgcagactg gaaacgtggg
    16681 agtgggaaac ggcgcatgtg atgtttgacg ccgctgaact taagctgccg ctgcttggct
    16741 atatggtgga aaacaccgtc ctgcaacagg cattgtggca ggcgctggaa gcgcatccga
    16801 aagtaacgtt acgtgtgcca ggctcgctga ttgcattaca tcgagataat gatcttcagg
    16861 agctggaact gaagggcggt gaagtgattc gcgcgaagct ggtgattggt gctgacggcg
    16921 caaattcgca ggtgcggcag atggcgggaa ttggcgttca tgcctggcag tatgcgcagt
    16981 cgtgtatgtt gattagcgta cagtgtgaga acgatcccgg cgacagcacc tggcagcaat
    17041 ttaccccgga cggaccgcgt gcgtttctgc cgttgtttga taactgggca tcgctggtgt
    17101 ggtatgactc tccggtacgt attcgccagt tgcagaatat gaatatggcg caactccagg
    17161 cggaaatcgc gaagcatttc ccgtcgcgtc tgggttacgt tacaccgctt gccgctggtg
    17221 cgtttccgct gacgcgccga catgcgttgc agtatgtgca gccggggctt gcgctggtgg
    17281 gcgatgccgc gcataccatc catccgctgg cggggcaggg ggtgaatctt ggttatcgtg
    17341 atgtcgatgc cctgattgat gttctggtga atgcccgcag ctacggtgaa gcgtgggcca
    17401 gttatcctgt cctcaagcgt taccagatgc ggcgcatggc ggataacttc attatgcaaa
    17461 gtggtatgga tctgttttat gccggattca gtaataatct gccgccgctg cgttttgtgc
    17521 gtaatctcgg gttaatggcg gcagagcgtg ctggcgtgtt gaaacgtcag gcgctgaaat
    17581 atgcgttagg gttgtagcct tacaacattg ccggatgcgt gccaaccgta ggtcggataa
    17641 gacgcggcag cgtcgcatcc gacattgaag gataagacgt gtcaacatcg cattcgacat
    17701 tgaatgaacg cagaaaagca aaaagctcgc cgaagcgagc ttttttaatg tggctggggt
    17761 acgaggattc gaacctcgga atgccggaat cagaatccgg tgccttaccg cttggcgata
    17821 ccccaacaaa ttggtttttg aatttgccga acgtattcga cacattcaga atttggtggc
    17881 tacgacggga ttcgaacctg tgaccccatc attatgagtg atgtgctcta accaactgag
    17941 ctacgtagcc agattg
//
LOCUS       DAZFWP020000103        17694 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFWP020000103 DAZFWP020000000
VERSION     DAZFWP020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.103"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            90..593
                     /locus_tag="ACHK0Z_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     CDS             90..593
                     /locus_tag="ACHK0Z_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU4503293.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(660..2117)
                     /gene="nuoN"
                     /locus_tag="ACHK0Z_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     CDS             complement(660..2117)
                     /gene="nuoN"
                     /locus_tag="ACHK0Z_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU4503294.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2124..3653)
                     /gene="nuoM"
                     /locus_tag="ACHK0Z_RS16650"
                     /old_locus_tag="pgaptmp_003330"
     CDS             complement(2124..3653)
                     /gene="nuoM"
                     /locus_tag="ACHK0Z_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU4503295.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3817..5658)
                     /gene="nuoL"
                     /locus_tag="ACHK0Z_RS16655"
                     /old_locus_tag="pgaptmp_003331"
     CDS             complement(3817..5658)
                     /gene="nuoL"
                     /locus_tag="ACHK0Z_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU4503296.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5655..5957)
                     /gene="nuoK"
                     /locus_tag="ACHK0Z_RS16660"
                     /old_locus_tag="pgaptmp_003332"
     CDS             complement(5655..5957)
                     /gene="nuoK"
                     /locus_tag="ACHK0Z_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU4503297.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5954..6508)
                     /gene="nuoJ"
                     /locus_tag="ACHK0Z_RS16665"
                     /old_locus_tag="pgaptmp_003333"
     CDS             complement(5954..6508)
                     /gene="nuoJ"
                     /locus_tag="ACHK0Z_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU4503298.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6520..7062)
                     /gene="nuoI"
                     /locus_tag="ACHK0Z_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             complement(6520..7062)
                     /gene="nuoI"
                     /locus_tag="ACHK0Z_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU4503299.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7077..8054)
                     /gene="nuoH"
                     /locus_tag="ACHK0Z_RS16675"
                     /old_locus_tag="pgaptmp_003335"
     CDS             complement(7077..8054)
                     /gene="nuoH"
                     /locus_tag="ACHK0Z_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU4503300.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8051..10777)
                     /gene="nuoG"
                     /locus_tag="ACHK0Z_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             complement(8051..10777)
                     /gene="nuoG"
                     /locus_tag="ACHK0Z_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU4503301.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10830..12167)
                     /gene="nuoF"
                     /locus_tag="ACHK0Z_RS16685"
                     /old_locus_tag="pgaptmp_003337"
     CDS             complement(10830..12167)
                     /gene="nuoF"
                     /locus_tag="ACHK0Z_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU4503302.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12164..12664)
                     /gene="nuoE"
                     /locus_tag="ACHK0Z_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             complement(12164..12664)
                     /gene="nuoE"
                     /locus_tag="ACHK0Z_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU4503303.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12667..14469)
                     /gene="nuoC"
                     /locus_tag="ACHK0Z_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             complement(12667..14469)
                     /gene="nuoC"
                     /locus_tag="ACHK0Z_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU4503304.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14563..15225)
                     /gene="nuoB"
                     /locus_tag="ACHK0Z_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             complement(14563..15225)
                     /gene="nuoB"
                     /locus_tag="ACHK0Z_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU4503305.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15241..15684)
                     /gene="nuoA"
                     /locus_tag="ACHK0Z_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             complement(15241..15684)
                     /gene="nuoA"
                     /locus_tag="ACHK0Z_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU4503306.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16315..17253)
                     /gene="lrhA"
                     /locus_tag="ACHK0Z_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             complement(16315..17253)
                     /gene="lrhA"
                     /locus_tag="ACHK0Z_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU4503307.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4415 a         4938 c         4588 g         3753 t
ORIGIN      
        1 aggcgggtcc agaaaaagcg acatgcacat tttattttcc ctcgtttatt ttaagatgcg
       61 aaacataaaa ttcaacggag gaaaaataaa tgggaatgat tggctatttt gcagaaattg
      121 actcagaaaa aattaaccaa ctgcttgagt ctactgaaaa acccttaatg gacaatattc
      181 atgacactct ttcaggatta cgccgactgg atattgataa acgctgggat tttttacact
      241 ttgggctgac aggcacctct gcttttgatc ccgcgaagaa tgatccgctg agtcgtgccg
      301 tgcttggtga acacagcctc gaagatggca tagatggctt cctgggattg acctggaatc
      361 aggagttagc cgcgacaatc gaccgactgg aagctttgga tcgaagtgag ctgcgtaaaa
      421 agttttccat taagcgactc aatgaaatgg agatttatcc gggagtgacc ttttcagaag
      481 agttagaggg gcaactcttt gcttcgatca tgcttgatat ggaaaagctg attagcgcgt
      541 atcgacgtat gctgcgacag gggaatcatg cgctgacggt tattgttggg tgatggtgtc
      601 agagcgccaa aaaaaaccgc cgaacatgtc ggcggctttc tgaattacga actgacagct
      661 tacatcagcg gcatggctaa ccgcacaatg ctaatcagcg gttgtggcca tacacccagc
      721 accagtacca acagtgcaga gatcagcaca acgataccgc ccgcgctgta ctgccagttt
      781 gatggtgcat cgcgacccgg ttgttccggg gcgtgaagat acaggctcac cgccacgcgc
      841 aggtagtagt agaggccgat tgccgaaccg acaaccacgg cacccaccag ccaccacaag
      901 tgtgcctgga caccgaccgc cagcacgtag aacttaccga taaagcccag cgtcatcggg
      961 ataccggcca gagacagcat catcaccgtc atcactgccg cgaggatcgg acgatgccag
     1021 aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg gctggacatc
     1081 aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccggccag gtaaacccct
     1141 accgcttcca tcgacatctc gccggtttgc agcgcaatca gcgccaccag cagatagccg
     1201 aggtgagaga tagatgagta accgagcaga cgtttgatgt tggtctggct caacgccatc
     1261 aggttaccga agatgatgga ggcaaaggcg ataatcgcca gtactacgcg aatcgcttcg
     1321 ctgtcaccca ccggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg
     1381 ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc
     1441 cacaggtgga acggcaccag agagagtttg aagccgaggc caacaatcat caggccgaaa
     1501 cctgccagca acagcggctc gttgagcata ccgtcgccga ggtttttacc caacgcgaca
     1561 aacgacaggt cgccagactg cgcatacacc agcgccatac cgaacagcag gaatgaagac
     1621 gctgcggcag aaaggatggt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa
     1681 gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt caataccgag gaacagagac
     1741 gccagatggt tggcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg
     1801 tagaactcat ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg
     1861 gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc
     1921 agcggcgtaa cgtccatagc gcccgcctgg ccgacaaacc agagcgaaac cagcgccgcg
     1981 ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag
     2041 agcatcacaa ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt
     2101 tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac
     2161 tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg atagaagccc
     2221 agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct
     2281 ggcagttcct ggctggcaat ctggcttttc gctttaccga agtaagcgcg atgtaacatc
     2341 gccagcgaat aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg
     2401 acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgccaggc
     2461 atcccaagcg ttgccaccgc aaagaacagc gacagtgctg gcagccattt catcttgctc
     2521 cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccacaa
     2581 agaataaaca gacccgccgc cgacaagccg tgcgcaatca tctggattac cgcgccctgg
     2641 taggccaact ggctgccggt gtagatggca atcagcacga agcccatgtg ggaaaccgag
     2701 gtgtaggcga tcagacgttt gatgtcggtc tgggcgaagg ccatccacgc accgtagaag
     2761 atgccgataa cacccagcca catagcgatt ggcgcaaact ctgccgacgc gttcgggaac
     2821 agcggcaggg agaaacgcag caaaccgtaa gcggcagttt tcagcaagat ccccgcgagg
     2881 tcaacggaac cggcggtcgg agcctgggag tgcgcatccg gcagccagcc atgcagcgga
     2941 accaccggca ttttgactgc gaaggcgatg aagaagccca gcattaacag gtattccaca
     3001 ccactggaca ttggcgtatt cagcagctct tcatagttga aggtccagac gccggtcgca
     3061 ttgtagtgaa caaaaaccag cgccaggatg gcgatcaaca tcaccagacc actcgcctgg
     3121 gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga ggctttatgc
     3181 ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac
     3241 aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg
     3301 aggtggaaga agccctgata tttttcgatc tctttccacg aacagagtac cgccagcaca
     3361 ccgagcagac cggtcagcac gaccatcagc agcgacagcc cgtcaatggc gagatgaata
     3421 gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg
     3481 gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc
     3541 aatcccatgg tgatcagcgc gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc
     3601 cagcacagga agccgccgat aaagggaatt aatattagcc agggtagtaa catggcgatc
     3661 tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca cgacagaaaa
     3721 ttctcttcgg actgggggta gtgtcggatg caccgcgaat gcggcttatc cgacctacgt
     3781 tcttatatgc caaggggcaa tcccacaatc cttaactcaa cgcagtacca tcaacagtgc
     3841 cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat agccgttctc
     3901 acttaacagc agacctttac ctgcaaagcg ggaaaggaca gccgggatgt tcatcattga
     3961 gttcagcgga tcgcgtttca gcaaccaggc aatacccagg aatggcttga cgaacacttt
     4021 gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag gtgcccagca gacggcccgg
     4081 cgcactgttg gcgatggagg tcaccagagt acgtttaccc agccacagcc aggctgccag
     4141 cagaatgccg accaccgcga ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg
     4201 cgccagttcc gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac
     4261 gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc ctttcacggc
     4321 gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga aggtgtagag
     4381 cgaggtcata aacgcaccca ccagacctgc caccatcaga ttgatatgac cattcgccat
     4441 cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg gcagtgccga
     4501 cagtgctgcg ccgcccacca ggaagcagag ataaaccagc ggaatagatt tacgcagacc
     4561 gcccatcttg aagatgttct gttcgtgatg gcaggccaga atgacggaac cggatgccag
     4621 gaacagcagc gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg catcccatgc
     4681 ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt aagcgagaac
     4741 acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc
     4801 aacaatgccc accagatgca gaacttccgg ggtcatcagg aacaggccgt gggtacgggc
     4861 gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg atcagcgcgg agacaggcgt
     4921 cgggcccgcc atcgcgtcag caagccatgt ctgcaacggc aactgcgcag atttaccgac
     4981 cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccat cagcaaagtg
     5041 cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat
     5101 gaacagtgcg aaagcgagga aaacgtcacc cacacgggtc acgacgaacg ctttcattgc
     5161 cgctgcgcca ttcttcggat cggtgtaata gaaaccgatc agcagatagg agcacaggcc
     5221 cacgccttcc cagccgaggt acatcagcag caggttgtcg gcaagcacca gaactaccat
     5281 gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag tagccctctt caccgcgcat
     5341 gtaccaggag gcgtacatgt ggatcaggaa acccacacca gtgaccactg agagcatggt
     5401 cagcgacagg ccgtccaaca ccaggttaaa accgatgtta aagtcgccta ccgacatcca
     5461 cgtccacagc ggctggctgt atgcctgctc gccgttagcg aagaaatcaa cgccgataaa
     5521 ggcggttacc agcgccgcca ggcccacaga gccaacgccg acgatcgccg aaacgttttc
     5581 agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca aaataatggt
     5641 taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag gttctggcga
     5701 cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttctgccgc cgcgaggctg
     5761 atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc ggcgaccaca
     5821 aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa cagcagattg
     5881 cgacggataa ccagaccggt taagccaaga acgaataaga ttgccgcgag gatcagtcca
     5941 tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg tctttacgat
     6001 tgctcagcac ttcacccgca cgctcttcac gaccgacgtg gaaggccaca accagacccg
     6061 cgagcagcag catagaagcc agttccaccg ccagtacgta aggcccgaac agcgtaatac
     6121 ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcgttaaca ccgaggatgg
     6181 cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc cacacctgcg
     6241 gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc atcatcacca
     6301 cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc gcaccggcga
     6361 agtaagcgcc tagtgagaag aacaccccgg aaatcgccag cagcgaaata atcaggtaca
     6421 gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt atggctatca
     6481 ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca ggctcttgac
     6541 gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga ttgccatacc
     6601 tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca gcagatcctc
     6661 tttctcgtaa accagatcct ggcgcttgta ttcccccatt tcgaaatccg gggttaactg
     6721 aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc gtgagaagtt
     6781 gatacggaaa aattccggat accagcgacc gtctttggtt tctgcctttt gcagcgagat
     6841 acagccgacc gggcaggcta ccgcacagag gttacaggct acgcaacgct cttcgccgtc
     6901 cgggtcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga ccggctcttc
     6961 cgggtacatt cgcgtttcgc gttttgcgaa cgcgtgcagg ccgatcatcc agatactacg
     7021 aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat tgccccttat
     7081 tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt cagcggcagg
     7141 cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa cgacgcacga
     7201 atcaaaatga acatcatcat aaagaacgcg gttttcagcg cgaaccagat gaatggcggt
     7261 aacaacgggc cttgccagcc accgaagaac agcgtcacca tcaatgcaga gatggtcaca
     7321 atcccgatgt actcacccac gaagaacaga ccgaacttca taccggaata ttcaatgtgg
     7381 taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg gtgacacacc
     7441 gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggatgac gttccacaca
     7501 tgcgcctggc tgttgacgat gtcggtcata ttgaatgaac cggcctgcgc caccacgccc
     7561 atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc acgcatcgca
     7621 ccgagcagcg agtatttgtt gttacttgac cagcccgcaa acagcaccgc gtaaactgcc
     7681 agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc
     7741 ggactgactg gcacaattgc aaaggccagc agcagcgagg taaaggcaat catcggtgcc
     7801 agggtaaaga tgacgcgatc cgagaacttc gggatccagt cttctttaaa gaacattttg
     7861 atcatatccg caaccaactg gagcgaaccg ccccagccaa cacggttagg tccgtaacgg
     7921 ttctggaaca gacccagcag gcgacgttcg ccaaagctca tgaatgcccc gcaggtgaca
     7981 accaccagca ggatcaccac cgctttgagg atggtcagca ggatctcaat cagttccggt
     8041 gatatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca gccagcaccg
     8101 gagcaatgcc ggacataccc atcggcaagc ccacctgccc tgccgtcagc ccttcggcga
     8161 tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt gtacctgcgt
     8221 ttacacccaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc ggcatacgac
     8281 tctggaagac cggagcacgc tgtgacaatt catcactgcc aaacaggtgg tagtacggcg
     8341 caatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg aagtaatcca
     8401 gaccattttc gctggtttca aacagacgca cgcccggatc gccaaagcgc agtttgccgc
     8461 ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcccgga gcccaggcaa
     8521 acggcacttg cgaacggtgc gcggtcggct ggttgttacc ttccatcgag aaggtgaaca
     8581 tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatattggcg cgcatggcgg
     8641 tacggccgct gtaacggtgc ggttcacggg ccagtttctg cccacgaata cggaatgtcg
     8701 catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca acagcgtcaa
     8761 tcacatggtc gagctgcgtc cagtccactt cacggctcag cagggtgctg tgcagcgagt
     8821 gtaaccaacg ccagctttcc agcatgacag ttttgctatc gtaataagcc ggatcgtaaa
     8881 cctggaagaa acgttgggcg cgaccttcat tattaattac cgtaccgtcg ctttcagcaa
     8941 agctggcagc ggaaagcacc agatgggcgt tttccatgat cgctgtgcgt tgatgatcaa
     9001 ccaccatcac cagcggcgct ttagccagcg cagcattcac gcgggtagcg gaagcgtgac
     9061 gatgcagatc gttttccagc accaccaccg cgtcggcgcg tccggtttcc agttcggtca
     9121 acgcttcttc aagcgaaccg ccacccataa tgcccagccc catgctgttg acggaacggg
     9181 caatcatggt gataccaacg tcagcgccgc gacctttcag ggctttcgcg acgttagccg
     9241 cagcctgaat cacctctaag ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg
     9301 cacctgccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt tcgataccgt
     9361 caaccgctgg tgcagagtta tccagcgcat gggcaatggc aaaacctaaa cgcgcctgat
     9421 cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc cagacgagtg tcatcaacgt
     9481 tggtaacaaa cagcggatgc ttagcacgtt gaccgatgtt gaggattgcc gcaatctgcc
     9541 agtcagccac tttctgtgct gccgccattt cgcgcgcttt acctttcacc gcctgacgca
     9601 ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc gcccagcacc agtaccgcat
     9661 cgtaagattc gatttcgcgc agagccggag tataaatgcc gccttcacgc agcactttca
     9721 gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc
     9781 ccaccagttc acgcagcgca aagttgcttt ccacgctggc acgcggagaa ccaataccga
     9841 tcactttctt cgactgacgc agaatatctg ccgcgccctg cattgcctgt tcggcgttga
     9901 gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatcc ttcaggttga
     9961 cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc gtaccgttgt
    10021 aacggttttc gatacgacgc agttcgccgt aacgttcacc ggggctgatg ttacagccga
    10081 tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct
    10141 cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa
    10201 attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt
    10261 aaacgcccag atctgtaccg tcagcgtaat ctttgtagta acgcacacag cggtagcagg
    10321 cgatgcagcg gttcatttcg tgagagatga atggccccaa atcctgatta cggtgggtac
    10381 gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacggtcata tcctgaagat
    10441 ggcagttacc gccttcttca cataccggac agtcgtgcgg gtggttggtc attaaccact
    10501 cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat
    10561 cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt
    10621 attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa
    10681 agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc gctccgttga
    10741 cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca gttttctcgg
    10801 tcagggaccg agcgttaatc gaaattcggt taccagcgct ctttcagcag gttcggctga
    10861 atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc aaattcttcg
    10921 cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa
    10981 gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc tcccggctga
    11041 ccttcgccac gctccagcgc acgcagaatt ttcacgctcc acggcagacc gtcgcggcac
    11101 ggcgtacacc agccgcagga ctcacgggcg aaaaactctt ccagattacg caccagcgac
    11161 accatgttga tctcatggtc aaccgccatc gccagcgccg tacccagacg gcttcccgct
    11221 ttaccgatac tttcgaattc catcggcagg tcaaggtgcg cttcagtcag gaaatcagtc
    11281 cccgcgccgc ctggctgcca ggctttgaat ttcagaccgt cacgcatacc accggcgtaa
    11341 tcttcaagga tttcacgcgc ggtagtaccg aacggcagtt cccacaggcc cggatttttc
    11401 acccgaccag agaagcccat cagcttggtg ccagcatctt tacttttcga gatgttctga
    11461 taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc gacgttgttg
    11521 acgcaggttg gtttacccca tgcaccggag gttgccggga aaggtggctt cgagcgtggg
    11581 ttagcgcgac gcccttccag ggagttgatt aacgctgttt cttccccgca gatgtagcgc
    11641 cctgccccgg tatggacgaa cagttcgaag tcaaaacctg ttcccataat atttttgcca
    11701 agcaaacctg cttcggtggc ttcggcaatg gcacggcgca gattcactgc cgcttcgata
    11761 tattcgccac gcaggaagat gtagccacgg taagctttca gcgcaaacgc ggagataagc
    11821 ataccttcca ccagcaggtg cggcagttgc tccatcagaa ggcggtcttt ataggtgccc
    11881 ggctccattt catcggcgtt acacagcagg taacggatgt tcatggattc gtctttcggc
    11941 atcaggctcc atttcaggcc ggtagaaaag cccgcgccgc cgcgcccttt cagaccagcg
    12001 tcttttacct gattaacgat ttcgtccgga gacagcccgg tcagcgcctt acgcgcccct
    12061 tcgtaaccgt ttttgctgcg atattcgtcc agccacactg gctgtttgtc atcgcgcaga
    12121 cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta taccgctcca
    12181 gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg atcatcatgt
    12241 ttggcccttt atcacagttc cccaggcagc aagttggcag cagcgtaaag cgaccgtcaa
    12301 atgtcgtttg ccctggtttg atgtttagct ttttctcgag cgccgcctga ataccctggt
    12361 aaccgttgat atgacagacc acgctgtcgc aataacggat cacatggcgg ccaaccggct
    12421 ggcggaagat ctggctgtag aacgttgcca cgccttcgac gtcgcttgcc ggaataccca
    12481 gcacatcggc gatcgcgtgg atcgcgccat ccggcaccca gccacgctgc ttctgaacga
    12541 ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa
    12601 tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt
    12661 gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat aaacaatcag
    12721 gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg
    12781 cgtgcggata cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt agtaactgtt
    12841 gatccccttc gtcgcctcaa tcatctggaa agattcattg gcaggcatca ccggccccca
    12901 cgacacttgc aagaagtggg tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg
    12961 cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt tgttgaggca
    13021 ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta cgcgggtgta
    13081 gcagtcagaa acaccaccac caaccgggat ttcaaagtcg aagttttcat agccagaata
    13141 aggacgcgcc ttacgcacgt cgaagtcgat cccggtggca cgcaggcccg cgccggtggt
    13201 gccccactcc agcgcctctt tcgcgccata ggcggcaacg ccctgggaac gacctttcag
    13261 aatggtattt tgcagcgctg ctttctcgta agacgccaga cgtttcggca tccagtcgag
    13321 gaactcacgc agcaggcgat cccagccacg cggcaggtcg tgcgctacgc cgccaatacg
    13381 gaaccacgcc gggtgcatac ggaaaccagt gattgcttcc accagatcgt aaattttctg
    13441 acgatcggta aaggcgaaga acactggcgt cattgcgccg acgtcctgaa taaaggtcga
    13501 gatatacagc aggtggctgt tgatgcggaa cagctcagaa agcattacgc gaatgacgtt
    13561 aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacgt aaggcatttc
    13621 gttaacgcag ccgccgaggt attcgatacg gtcggtatac ggaatgtagc tgtgccagga
    13681 ctggcgttcg cccattttct ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc
    13741 gacaatctct tcgccatcga gctgcaaaac gatacggaaa gcaccgtgcg ccgacgggtg
    13801 gttcggaccg aggttgagga acatgaagtc ctcgttttca gtaccacgct tcatccccca
    13861 ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt tggtcagctc
    13921 aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca
    13981 ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaatgtaa tgccaaacag
    14041 atcccaggtt tcacgctcat accagttagc gttcgggaac agtttggtga aggtcggtac
    14101 gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac gatcgataga
    14161 aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag
    14221 acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca gtttctttaa
    14281 gaaatcgcca acttccagta attgttcacg cttgatccac acaacgggaa ccccggtgcg
    14341 agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc caattaccgg
    14401 atcatcaaga tgatcgcggg tctgccaggc gggttcttgc gcggttaagt cggtcatatt
    14461 gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc gaaatagatt
    14521 taggaataag cgaagaaaaa tccctttgcc gacaggcgca aattaaatct cgtcaggtgt
    14581 acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg attgcatatt
    14641 ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt ctttgccgat
    14701 agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg
    14761 gatatacaca tcaaccggga tgaatttatc gacgccctgc acaacggaat aaatatcgta
    14821 cataccacca gagttggcac aggcacccat tgagataacc cattttggtt ccagcatctg
    14881 gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg caaccaccat
    14941 caggtcagcc tgacgcgggg aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg
    15001 caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga agttatacgg
    15061 ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca taaacacgtt
    15121 tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca gggggtaacg
    15181 gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg
    15241 ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg
    15301 cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg ccagtaacac
    15361 aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga tagaggttga
    15421 ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg ccaccagata
    15481 aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg attcaaacgg
    15541 cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag
    15601 gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat gagcgatgac
    15661 ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgccag attcactgct
    15721 cttcacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa ccttacaatc
    15781 actgtagaaa ttcttttata cagctaattg atgtggtctt ttactccttt ctataacctt
    15841 ttgtcaactt taacaaaagt ttcttcacat tagtttacat aatatcaaca ccattagcat
    15901 ttaatgccct ttcaccccag atccttgacg actccaggat aattagatgt tgttgaatcg
    15961 tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt
    16021 cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat tccataacaa
    16081 aaacgcagca acaaatttac gtatttttta acatcattgt agcaggtgat ttttttcagg
    16141 cgattatttg tgcgtacggg gcgtgaatct ctggtgattg aaaaatgaac agttttgtac
    16201 gttctgcacg atgtgaagaa ggttctttac taaaaaaaag ccgctggggt ttaaaacacc
    16261 cccagcggct cgttttttac actattgtct caggaattat ctatcgtccg tcgattactc
    16321 gatatccctt tcaatcaaca atgaatcatc cccttccgga gcagatattg ggctgtattg
    16381 ccacggatta tggtagctct ccatcgcctg atagatcacc tgagccagct cattattact
    16441 ggacgggtca tagcacagca aatattcggt atcgggtagc ggcggtaagc catcgactcc
    16501 actcagcaca cgcaggtctg gactcatcat ctcgaccggc cttgccgtca cgccaagacc
    16561 ggctttcact gccgcacgaa cggccggaag cgtcgaggcg acataagcca gtcgccaggg
    16621 aatatctgct ttattaagcg tcgccagcac catatcgcga aacggacttg gatcatccag
    16681 caatacaaga gggatcggct cacctttttg caatacgtat tccgctgcgc agtaccagtg
    16741 tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg tggttaccat
    16801 caaatccact tcctgggatt ccagcatttc tgccatatag gcattacgtt taacgcggac
    16861 atccagcgct aatttcggat aaaccgaact cacgcgattt aacaggaaag gtaagatcgt
    16921 atcggcagat tcatctgaag caccgatagt taacacgccc tgaagattac tgaacattaa
    16981 tgatgagcag gcctcatcat taaaacgcag gattttcctg gcgtaaccaa gaagttgaat
    17041 gccatgttca gttaacagtt tgttgcgacc gtgacgagcg aacagttctt tcccaacgag
    17101 ttgttccaga cgctgcattt gctgacttac ggcggactga gtacgacaca cagcggcagc
    17161 tgcggcagca aaagtgttca gatcggcaac agcaacaaat gttctcagca gatcgaggtc
    17221 gaggttaatt atcggacgat ttgcacttat catatattat cacttactgg cggctcatac
    17281 tgagctggtt aatgctgtgc acacacaaac aagcaattcc atttgtaatg tgcctccctg
    17341 gcagtttcat ccggaacccg gacgaaagta aaaatgcata tgagttgcac taaaaaagcg
    17401 actcacattg ttccgttata atgcctgaag tagatcacag aatatatctt cagggatcgc
    17461 atatctatta agttactcac tcttttctat ttatgacatg cgcgtgtttg tataaatgta
    17521 aatgtaagtc cttgttccac tctcgtgcag catcgctggt catacgcgaa cacgtacaaa
    17581 cagcaatggt gaggcggcat caagagcagg gatccgtcat ttatgcgagc attttacccc
    17641 aaaaaccttt tatttataag ggtcattgcg aattatctga tgcaaagtta tgtt
//
LOCUS       DAZFWP020000104        17558 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFWP020000104 DAZFWP020000000
VERSION     DAZFWP020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.104"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            79..1218
                     /gene="arnB"
                     /locus_tag="ACHK0Z_RS16715"
                     /old_locus_tag="pgaptmp_003343"
     CDS             79..1218
                     /gene="arnB"
                     /locus_tag="ACHK0Z_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU4503308.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1222..2190
                     /gene="arnC"
                     /locus_tag="ACHK0Z_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             1222..2190
                     /gene="arnC"
                     /locus_tag="ACHK0Z_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU4503309.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2190..4172
                     /gene="arnA"
                     /locus_tag="ACHK0Z_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             2190..4172
                     /gene="arnA"
                     /locus_tag="ACHK0Z_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU4503310.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4169..5059
                     /gene="arnD"
                     /locus_tag="ACHK0Z_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             4169..5059
                     /gene="arnD"
                     /locus_tag="ACHK0Z_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU4503311.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5059..6711
                     /gene="arnT"
                     /locus_tag="ACHK0Z_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             5059..6711
                     /gene="arnT"
                     /locus_tag="ACHK0Z_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU4503312.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPQLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6708..7043
                     /gene="arnE"
                     /locus_tag="ACHK0Z_RS16740"
                     /old_locus_tag="pgaptmp_003348"
     CDS             6708..7043
                     /gene="arnE"
                     /locus_tag="ACHK0Z_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU4503313.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7043..7429
                     /gene="arnF"
                     /locus_tag="ACHK0Z_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             7043..7429
                     /gene="arnF"
                     /locus_tag="ACHK0Z_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU4503314.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7423..7689)
                     /gene="pmrD"
                     /locus_tag="ACHK0Z_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             complement(7423..7689)
                     /gene="pmrD"
                     /locus_tag="ACHK0Z_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU4503315.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7799..9154)
                     /gene="menE"
                     /locus_tag="ACHK0Z_RS16755"
                     /old_locus_tag="pgaptmp_003351"
     CDS             complement(7799..9154)
                     /gene="menE"
                     /locus_tag="ACHK0Z_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU4503316.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9151..10113)
                     /gene="menC"
                     /locus_tag="ACHK0Z_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             complement(9151..10113)
                     /gene="menC"
                     /locus_tag="ACHK0Z_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU4503317.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10113..10970)
                     /gene="menB"
                     /locus_tag="ACHK0Z_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             complement(10113..10970)
                     /gene="menB"
                     /locus_tag="ACHK0Z_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU4503318.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10985..11743)
                     /gene="menH"
                     /locus_tag="ACHK0Z_RS16770"
                     /old_locus_tag="pgaptmp_003354"
     CDS             complement(10985..11743)
                     /gene="menH"
                     /locus_tag="ACHK0Z_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU4503319.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11740..13410)
                     /gene="menD"
                     /locus_tag="ACHK0Z_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             complement(11740..13410)
                     /gene="menD"
                     /locus_tag="ACHK0Z_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU4503320.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13499..14794)
                     /gene="menF"
                     /locus_tag="ACHK0Z_RS16780"
                     /old_locus_tag="pgaptmp_003356"
     CDS             complement(13499..14794)
                     /gene="menF"
                     /locus_tag="ACHK0Z_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU4503321.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14873..15178)
                     /gene="elaB"
                     /locus_tag="ACHK0Z_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     CDS             complement(14873..15178)
                     /gene="elaB"
                     /locus_tag="ACHK0Z_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU4503322.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15233..15694)
                     /locus_tag="ACHK0Z_RS16790"
                     /old_locus_tag="pgaptmp_003358"
     CDS             complement(15233..15694)
                     /locus_tag="ACHK0Z_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4503323.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15759..16676
                     /gene="rbn"
                     /locus_tag="ACHK0Z_RS16795"
                     /old_locus_tag="pgaptmp_003359"
     CDS             15759..16676
                     /gene="rbn"
                     /locus_tag="ACHK0Z_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU4503324.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16864..>17558
                     /locus_tag="ACHK0Z_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             16864..>17558
                     /locus_tag="ACHK0Z_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HGU4503325.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTG"
BASE COUNT         4132 a         4734 c         4428 g         4264 t
ORIGIN      
        1 ctccaaatgg caaaatatac acaacactct ttatagcaaa tataagtgga caggtattca
       61 atggcggaag gaaaagcaat gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg
      121 gaggaactcg ctgcagttaa agaggttctc gaatccggtt ggctcacaac cggtccgaag
      181 aatcaggcgc ttgagcaagc cttttgccag ttgacgggaa atcagcatgc catcgcggtc
      241 agttcagcca ccgccggaat gcatatcacg ctaatggcgt tggaaattgg caagggcgat
      301 gaagtgatta cgccttccct gacctgggtt tcaaccctca atatgatttc cttgttgggt
      361 gcaacgccgg taatggtgga tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc
      421 gagtcagcca taacgccacg cactaaagcc atcattccgg tgcattatgc cggtgcgcca
      481 gcagatattg acgccattcg cgccattggc gaacgttacg gcatcgcagt tatcgaagat
      541 gctgcccatg ccgtcggtac gtattacaaa gggcgacata ttggcgcaaa aggtaccgct
      601 attttttcat ttcatgccat taaaaatatt acctgtgcgg aaggtggcct gattgtaact
      661 gataatgaaa accttgcccg ccagctacgg atgctgaaat ttcacggtct gggtgtcgat
      721 gcctatgaca gacaaacctg gggccgtgca ccgcaggctg aagtcttaac accgggctat
      781 aagtacaatc tgaccgatat taacgccgca attgccctga cacagttagc aaaattagag
      841 cacctcaaca cccgtcggcg cgaaattgcc cagcaatatc agcaagcact ggcagctctc
      901 ccctttcagc cattaagcct tcccgcctgg ccgcacgttc acgcctggca tctgtttatt
      961 attcgtgtcg atgaacaacg ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa
     1021 gaaagaggca ttggtaccgg gttacatttc cgcgccgctc acacacaaaa atattatcgc
     1081 gagcgttttc ccacgctgtc gttaccgaat accgaatgga atagcgaacg catctgttct
     1141 ttgccgctgt tcccggatat gactaccgcc gatgccgacc gtgtcatcac agcccttcag
     1201 caactcgcag gacaataagc catgtttgaa atccaccctg ttaagaaagt ctcggtggtt
     1261 attcccgttt ataacgagca ggaaagctta ccggaattaa tcaggcgcac caccacagcc
     1321 tgtgaatcgt tggggaaaga gtatgagatc ctgctgattg atgacggcag tagcgataat
     1381 tccgcgcata tgctggtcga agcctcacaa gcggagaaca gccatattgt gtctattttg
     1441 cttaaccgca attacgggca acattcagcg attatggcgg gattcagtca cgttaccggc
     1501 gacttaatta ttacccttga tgccgatctc cagaatccgc cagaagaaat cccccgcctg
     1561 gtggcaaaag ccgatgaagg ttacgacgtg gtagggactg tacgccagaa ccgccaggac
     1621 agctggtttc gtaaaacagc ttcgaagatg attaaccggc ttattcagcg caccaccggc
     1681 aaagcgatgg gtgactacgg ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg
     1741 atgttgcact gccatgaacg tagcaccttt atcccgattc tggcgaatat cttcgcccgc
     1801 cgtgccattg aaattccagt acatcattcc gagcgtgagt ttggtgaatc caaatacagt
     1861 tttatgcgcc tgattaattt gatgtacgac ctggtgacct gccttaccac aacgccgcta
     1921 cgtatgctga gtctgctcgg cagcattatt gcgattggag gttttagcat tgcggtgctg
     1981 ctggtgattt tacgcctgac cttcggacca caatgggcgg cagaaggcgt ctttatgcta
     2041 tttgccgtgc tgtttacttt tattggcgct cagtttatcg gcatgggatt actcggtgaa
     2101 tatatcggca ggatctacac cgatgtccgc gcccgccccc gctattttgt tcagcaagtt
     2161 atccgtccat ccagcaagga aaatgaataa tgaaaaccgt cgtttttgcc taccacgata
     2221 tgggatgcct cggtattgaa gctctgctgg ctgccggtta cgaaattagc gccattttta
     2281 cccataccga taatcccggt gaaaaagcct tttatggttc ggtggctcgt ctggcagcgg
     2341 aaagaggcat tccggtttat gcgccggatg acgttaatca tccgctgtgg gtggaacgca
     2401 ttgcccaact gtcgccagat gtgattttct ctttttatta tcgccatctt atttgcgacg
     2461 aaattttgca gctcgctccc gcaggtgcat ttaatctgca tggttcgctg ttaccaaaat
     2521 atcgtggtcg cgcgccgctg aactgggtgc tggtcaacgg tgaaacggaa actggcgtta
     2581 cattgcaccg aatggtgaaa cgtgccgatg ccggggccat tgtggcgcaa ctgcgcattg
     2641 ccattgcgcc agacgatatc gctattacgc tgcatcataa attgtgccat gccgcgcgcc
     2701 agctactgga acagacatta cccgccatta aacacggtaa tattctggaa atcgcccagc
     2761 gcgaaaacga agccacctgt tttggtcgca gaacgccgga tgacagtttc cttgaatggc
     2821 ataaaccggc atccgtactg cacaacatgg tacgtgccgt tgccgatccg tggccgggtg
     2881 ccttcagcta tgttggcaat cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg
     2941 ccagcaaagc acagccgggg agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg
     3001 atggcgcgct ggaaatcgtc accggacagg cgggcgacgg cattactatg cagggctcgc
     3061 aattagcgca gacgctgggc ctggtgcaag gttcacgctt gaatagccag cctgcctgca
     3121 ccgcccgacg ccgtacccgg gtactcatcc tcggggtgaa tggctttatt ggcaaccatc
     3181 tgacagaacg cctgctgcgc gaagatcatt atgaagttta cggtctggat attggcagcg
     3241 atgcgataag ccgttttctg aatcatccgc attttcactt tgttgaaggc gatatcagta
     3301 ttcattccga atggattgag tatcatgtca aaaaatgtga tgtcgtcttg ccgctggtgg
     3361 cgatagccac gccgattgaa tatacccgca acccgctgcg cgtatttgaa ctcgattttg
     3421 aagagaatct gcgcattatc cgctactgcg tgaagtaccg taagcgaatc atcttcccgt
     3481 caacttcaga agtttatggg atgtgtagcg ataaatactt cgatgaggac cattctaatt
     3541 taatcgtcgg cccggtgaat aaaccacgct ggatttattc ggtatcaaaa caattacttg
     3601 atcgggtgat ctgggcctat ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt
     3661 ttaactggat gggaccgcga ctggataacc ttaatgcggc acgaatcggc agctcccgcg
     3721 ctattacgca actcattctc aatctggtag aaggttcacc gattaagctg attgatggcg
     3781 gaaaacaaaa acgctgcttt actgatattc gcgatggtat cgaggcgtta taccgcatta
     3841 tcgaaaatgc gggaaatcgc tgcgacggtg aaattatcaa cattggcaat cctgagaacg
     3901 aagcgagcat tgaggaactg ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc
     3961 gccatcattt cccaccgttt gcgggctttc gcgttgtcga aagtagcagc tactacggca
     4021 aaggatatca ggacgtagag catcgtaaac cgagcatccg caatgcccac cgctgcctgg
     4081 actgggagcc gaaaattgat atgcaggaaa ccatcgacga aacgctggat ttcttcctgc
     4141 gcaccgttga tcttacggat aaaccatcat gaccaaagta ggcttacgca ttgatgtcga
     4201 tacctttcgt ggcacccgtg aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa
     4261 tattcaggcc agcatttttt tcagcgtcgg cccggacaat atgggccgcc atctctggcg
     4321 actggtgaag ccacagtttt tgtggaagat gctgcgctca aatgcggcat cgctttatgg
     4381 ctgggatatt ttactggcag gtacggcctg gccaggtaaa gagattggtc atgccaatgc
     4441 cgatatcatt cgtgaagcgg ctaaacatca cgaagtcggc ctgcacgcct gggatcacca
     4501 tgcctggcaa gcccgtagcg gtaactggga tcggcaaaca atgatcgacg atattgcacg
     4561 cggtcttcgc actctggaag agattatcgg tcaaccggta acctgttctg ccgctgcggg
     4621 ctggcgtgcc gaccagaagg tgatcgaagc aaaagaagcg ttccatttgc gctacaacag
     4681 cgattgtcgt ggggccatgc cgttccgtcc attgctcgaa tcaggaaacc ctggcactgc
     4741 gcaaattccg gtgaccttac ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga
     4801 agattttaac ggttggttac tcaaccgcat cctgcgagat aaaggcacgc cggtttatac
     4861 cattcatgca gaagttgaag gctgcgctta tcagcataat tttgtggatc tcctcaaacg
     4921 cgcagctcag gaaggcgtga cattttgccc tttaagcgaa ctgttatcag agacgttgcc
     4981 gctcggacaa gttgttcgcg gaaatattgc cggacgtgaa ggctggctgg gttgccaaca
     5041 aattgcgggt agtcgctgat gaaatcggta cgttacctta tcggcctctt cgcgtttatt
     5101 gcctgctatt acctgttacc gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt
     5161 tatgcggaaa tcagtcgaga aatgctggca tccggcgact ggattgtgcc ccaactgtta
     5221 gggctacgtt atttcgaaaa acccattgcc ggatactgga ttaacagcat tgggcaatgg
     5281 ctatttggcg cgaataactt tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact
     5341 gccgcgctgg tgacctggtt tactctgcgc ttatggcgcg ataaacgtct ggctctactc
     5401 gccacagtaa tttatctctc attgtttatt gtctatgcca tcggcactta tgccgtgctc
     5461 gatccgttta ttgcattctg gctggtggcg ggaatgtgca gcttctggct ggcaatgcag
     5521 gcacagacgt ggaaaggcaa aagcgcagga tttttactgc tgggaatcac ctgcggcatg
     5581 ggggtgatga ccaaaggttt tctcgccctt gccgtgccgg tattaagcgt gctgccatgg
     5641 gtagcgacgc aaaaacgctg gaaagatctc tttatttacg gctggctggc ggttatcagt
     5701 tgcgtactga cggttctccc ttggggactg gcgatagcgc agcgggagcc tgacttctgg
     5761 cactattttt tctgggttga gcatattcaa cgctttgcac tggatgatgc ccaacataga
     5821 gctccgttct ggtactacgt gccggtcatc attgccggta gcctgccgtg gctgggatta
     5881 ctccccggtg cactgtacac aggctggaaa aaccgcaagc attccgcaac cgtctatttg
     5941 ttgagctgga cgataatgcc gctgctgttt ttctccgtcg ctaaaggtaa attgcccacc
     6001 tatattcttt cctgctttgc atctctggca atgctgatgg cgcattacgc tttgctggca
     6061 gcaaaaaata atcctctggc gctgcggatt aatggctgga ttaacatcgc ttttggcgtc
     6121 actggcatta ttgccacatt tgtggtctcc ccgtggggac caatgaacac gccggtgtgg
     6181 caaaccttcg agagctataa agtcttttgt gcctggtcga ttttttcgct atgggcattt
     6241 ttcggctggt acaccttaac aaacgtcgaa aagacctggc cttttgccgc gctttgcccg
     6301 ctggggctgg cgttgctggt aggattttca attcctgaca gagttatgga aggaaaacat
     6361 ccgcaatttt ttgtcgagat gacacaagaa tcacttcagc caagccgcta tattcttact
     6421 gatagcgtcg gtgttgccgc aggtctggca tggagcctgc aacgcgatga catcatcatg
     6481 tatcgccaga caggtgagtt gaaatacggc cttaattatc cggatgcgaa agggagattt
     6541 gtcagcggtg atgagttcgc aaactggctt aatcaacatc gtcaggaggg gattattact
     6601 ctcgtgcttt cggttgaccg cgatgaagat atcaacagtc tcgccattcc gcccgcagat
     6661 gccatcgatc gtcaggagcg tctggtgctg attcagtatc gtcccaaatg atctggctaa
     6721 cattagtctt tgccagcttg cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct
     6781 gctttgtggc gataaacaaa cggcgcaaac atatcgtgct gtggctggga ctggcgctgg
     6841 cttgtcttgg tcttgccatg gtgctctggc tgctggtctt gcagaacgta ccggtaggca
     6901 ttgcctaccc gatgttaagt ctgaattttg tctgggtgac gctggctgca gtaaaactgt
     6961 ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc gttcattatt ggtggcattg
     7021 tgatcctcgg gagtacggtg taatgggcct gatgtgggga ttattcagcg tcattattgc
     7081 ctcagttgcg caattaagcc tgggttttgc ggcgagtcat ctgccgccga tgacgcactt
     7141 atgggatttt atcgcggctc tgttagcttt tggcctggat gcccggatac tgttactcgg
     7201 gctgctgggt tatctgcttt ccgttttttg ttggtataaa acgctacata aacttgcgct
     7261 aagcaaagcc tatgccttgc tcagtatgag ttatgtgctg gtatggattg cttcaatggt
     7321 acttcccggc tgggaaggga ctttttcgct gaaagcacta ctgggagtag cctgtattat
     7381 gagcgggttg atgctgattt tcctgcccat gacaaaacaa cgttactgag ttttccctgc
     7441 cactttacac tgccgttccc actcatcggg gctataactg cttgccgaga ggactttcac
     7501 cgtatgcagc ttttcacgat taatacaata aagcgcattc tgcaaaggcg agagtaaatc
     7561 ccccactttc acggcgaagt cactcttcac ttcggcgatc atttttatcg cgccgccagc
     7621 atcgcagagc ataagtacat gtctgttatc ttgtttattg cagcacgatt ttttgaccag
     7681 ccattccatt gcattatcct gtttgctaag agttttcaca tcaatttaca gattaacaga
     7741 taaactcttt atcaacggca atggaatggc agaaatgaga agtgaaacgg agggttaatt
     7801 attgctgacg ttgcacccac tcttttagcg cctgacgtga aattttaata ccgccgtttt
     7861 tcagttcagg cggcaacgtt aaccaacgca ccggttgctg aaaactggcc agcttatctt
     7921 tcacccattc actaagatca acgctctcgt ggtcatactc catcaccgcc accggtcgat
     7981 gaccaaactc cttgtcggca acggggacga taaacacctg caaaaccgca ggatgtgcag
     8041 caattacgcg ctcgacttct tccggctgaa taccctcacc gccactgaag aacagattgt
     8101 ctaaacgtcc gacaatggtc agcttgccat tatgcatctc accgcgatcg cgcgtagcgt
     8161 accagccttc gtcattaacc agtgaaacca gttgcccgtt acgccagtaa ccttctgcca
     8221 tactggcagc ccgcagccac acttcattat taacgatttt cacttcccga cccggcagcg
     8281 gcgaaccaac gtctgccagg ccgtcggctt ctttcgcaca caccgtggag gcaaactcgg
     8341 tcagaccata gccgcaaaag caacgaatcc cctgctcgcg cgcctgttcc gtcaactcga
     8401 ccgggatagc cgcgccgcca agtaacaccg ctttcaggga aacggaactg cggttaacca
     8461 gcaaacgcca gagttgtgtt ggcaccagtg aagcgtgagt acagcctgcc agcatttgct
     8521 ccaatggctg tttatcacgc accgtcatcc gcgcaccagc gtataaccag cgccacataa
     8581 ttccctgacc ggagacgtga aacagcggta aagagagcaa ccagtcatcg tgatcgccaa
     8641 acggaatcaa cgataacacc ccttcggcac tggcaagatg ggcctgataa gtatggacag
     8701 cggctttcgg caacccggta gaacccgagg tcaacgtcat tgagcacaga cgcgtcggct
     8761 gccacgtagc ggcatgtgcg ccttcaaccc gctgaatgtg cagcgacgtt aacgccggaa
     8821 acgtgttttc cccatccggc accagcgcaa attgcagcgt cagattgggc agcaattctt
     8881 caagcaacgg ttgcggcagc tgagggttca cgggcaacac ccgcgccccg cattgcagta
     8941 acgccagcca ggcgagcagc gtttgcggcg tattccacgc ccgcaacatc acgccgctgc
     9001 cctcaacaac gccctgcgcc gcaaagcctg aggctaattc atcgacgcga gcacaaagct
     9061 cgcgccagtt aagttgctcg tcattaagac gtaaggcgat ggcttctccc cgcacttgcc
     9121 gccagtgacg ccacggccag tcagagaaga tcataacaac cgctccagtg catcaacttc
     9181 cacgacaggc agcgtgctac ccggccagcg acgtacctgc tgcgcctgca tcagatccag
     9241 cgtgtccagc cctggaatgg tgtccggcgt taaccaggcg gcaatccgcg ccagttgcgt
     9301 taagcctaag ctcgattcaa tggaagaact gatcaccgcc gtcagcccca gcgcgtgcgc
     9361 cgcctgtacc tgctcgcgta ctttctccag actgcccgtg agcgtgggtt tgataactac
     9421 cgcgcgcacg ccctcttcag ccacaaaggc aaaatccggc tcgcgcaagc tttcatccca
     9481 ggcaatggca atgccggttt cacgggcaaa cgctcgcgaa tcatcgcggg ttttgcacgg
     9541 ctcttcgaga aacgcgatgc ggtggcgata atccgggtta acatatttgg caaactgctg
     9601 gcctttcagc ggtgtccagg cgcgatttgc gtcaagacgc aaatgcagat ccggaattgc
     9661 ctccagcaac agattaacca ccatgccgtc gcgcacggct tcgtacaatc ccactttgac
     9721 cttcgccact ttctcgcctg gcatatcagc aagtttgagg atcagatcgt caggatcgcc
     9781 attacacagt ggtgccgcac ggtaattggc cgcctgcggg agcgtatctg ccagctccgc
     9841 caatgcacag cttacgccaa aggcaacgga aggcatctgc ggtagctcgc aatcgcctgc
     9901 cagccagtta tttacccagg caagcagcac gctttgcgcc tcttcccagg tttcctggct
     9961 gaagcccggc agtggggaga tctcccccca cccttcgcgc tcgccctcac gcaggcaaac
    10021 gtacagcccg tcgcgggttt ttaaccgcct gtcgcgcaga accacccccg cgtccatggg
    10081 gatctgccag cggtatacct gcgcgctacg cattacggat tccgtttaaa tttgctgaaa
    10141 tcaggctgac gtttctggtt gaaagcgttg cgaccttcct gaccttcttc cgtcatgtag
    10201 aacagcatgg tggcgttgcc cgccagctcc tgcagccccg cctgcccgtc acagtcggcg
    10261 ttcagcgcag ctttcaggca gcgcagcgcc atcgggctgt tttgcagcat ttcgcggcac
    10321 caacggacgg tttctttttc cagatccgcc agcggtacca cggtgttgac aaggcccata
    10381 tccagcgcct gttttgcgtc gtactgacgg cacaggaacc agatttctcg cgcttttttc
    10441 tgcccgacga tacgagccat gtaggaagcg ccccagccgc cgtcgaagga accgactttc
    10501 gggccagtct gaccgaagat ggcattatct gctgcgatag tcaggtcgca catcatgtgc
    10561 agaacgtgac caccgccgat ggagtagcca gccaccatcg cgacaaccgg tttcggacag
    10621 gtacggatct gacgctggaa gtccagcacg ttcaagtgat gtacgccgga atcatcttta
    10681 tagccgccgt aatcaccacg cactttctgg tcaccaccgg agcagaacgc tttatcgcct
    10741 gcgccagtca gaatgatcac gccgatattg tcgtcataac gcgcatctgc cagtgcctgg
    10801 atcatctctt ttaccgtcag aggacggaag gcattgcgca cctgcggacg attaatggtg
    10861 atttttgcga taccgtcggt ggatttttca taacgaatgt cctcgaaacc ttcggagcag
    10921 tcgtgccatt caaccggtgc gtaaagcatt gcttcatcag gataaatcat attgtgtcct
    10981 ttagtcagaa acgcaagatc tgcgccagac ttgcgattac gccagcggga ttttcccgat
    11041 gcgcgttatg tccggcgcga ggaatgacat ggcagtcggc agccagttcc gccgccaggg
    11101 cgcggaattt gctgtcacgt tcaccacata aataataaaa cgcaaatgtg cgggcgctaa
    11161 ggttagcacg taaatcaggc tggacggcga gagaagtcgc ctccagcatg gcggcaaggg
    11221 ttgcgccatt attgttgctg cgcagcgcca ccagctcccg gcgttgatcg tcattgagcg
    11281 aggcaaaaac aggctgttga taccagtcgg caaataccgc cgttaacggt tctgtgcgaa
    11341 aacgctgcgc ccattggcga tcggaacgct gacgttccgc acgttgttca gcattttgca
    11401 gccccggatg cccgccttcg acaataaccc cacaaagccc cgccagcccc tggcaagccg
    11461 ccatcatcgc cacccgtccg ccaagcgagt accccactag ccaaaagtca aggatgttgt
    11521 aactaaccaa ggttttacgc agtaagtcgg tgacatcatc aaatccatcg acgctaatcg
    11581 ccgccgaacc accatgacct gggagatcaa catacaaccg tgagtagtcg gcaaacgcct
    11641 cgcccacttc ttgccattcg tggcaatcgc cggaaaaacc gtgcaaaaac accagccagg
    11701 gtaaacctgg ttttccgtgt tttgcctgcg cgtgcaggat cataaatggc ttacctgcgc
    11761 cagaagttgc tggagcgttt gcgcaccatc ggtgtcgtta accaccattt caatcaccgt
    11821 ggtggttggc gtgcgccagg cgtcggcaaa tgccgtttca agttcctgcc agttttgcgg
    11881 acgatgatat ttcagctcga acatcgcggc ggcgtgctca aaatggacgt tttgcggcat
    11941 cagatagaaa cgctcacgct cgctttgcgg cgttggcaac agcgagaaaa tttgcccgcc
    12001 gttgttgttc accacaatta ataccagcgg cgcagaaacc tgacgcaata acgccagcgc
    12061 gttgagatcg taaagtgcgg agagatcgcc cacaatcgcc agcgtcggtt tgccgcttgc
    12121 ccgctgaacg cctgcggcgg tcgaaagcag tccgtcgata ccgctggccc ctcggttgct
    12181 gtacaccggg taacctgctg gaagttgcga aagcgcatca atcagacgta ccaccaggct
    12241 gttaccaaca aacaattgcc cctgttcagg cagatagtcg cagatgcgat gcgccagttg
    12301 cgcttcgcca aacgcatcac ggcgggcaat aaccgcctgc attgcctgtt ccgccaggcg
    12361 cgggatttca acgcaccagg gctggcgttt ttctgccgga tgcagctcca gccagtcggc
    12421 aatattggca attaagcgac gtccgcgatg gtgtgccgga tcaagtcgcc cttcaatgtc
    12481 atcaacaatc cagtactctt ctggttcaca gcttgcctgc cattgcagga gccgtttgcc
    12541 cgtcaggctg cttcccagtt gcaccacaat ttgcgcctgc tgcagctcgc tggtcgcttt
    12601 ggcattgcct aaccaaagat cggcacacgg cagcggctgc ccggtttgtg acagcacatc
    12661 gccaatcagc ggccagccaa gagtttgcgc ccacagggca acttttttgc cctcttccgc
    12721 actcatgcgc ccggcaacca ccacgccgcg cttttgtcgc cagaagaacc agtcgcgctg
    12781 tttttcactt tccagacgag gcgcttcacg cagccacggt ttgtcgtcct gccaccagtc
    12841 acccagacgc tgttgccagc taagcccggt atcgtccatt tcgccataca gcggttcagc
    12901 aaacgggcag ttgatatgga ctccccccgc atgaagcgta ccgagagcgt ggtcgatggt
    12961 agaaaccagc caacgtgcgg ggatatcccg ggtcgggcgc ggcaatgaaa tactgtgcgt
    13021 ggggtgagag gcgaacattc ccggctggcg aattgcctga ttcgcgccgc agtcaattag
    13081 ctccggcggg cgatcggcgg ttaacaggat cagtttttct ccggttaacc cggcttcaat
    13141 cagtgcagga tagagatttg ctaccgccgt gccggaggtc acaatcaccg ccaccggctg
    13201 cttgctgact ttcgccagcc ccagcgccag atgccccaac ccacgctcat cgaaatgggt
    13261 gtggtgaatg aatgcggaat tctccgccgc cgctaacgtt aacggtgtag aacgcgagcc
    13321 aggggcgata cagatgtgtc tgacgccgtg acgcgttaat gcttccagaa tgaccgccgc
    13381 ccagcgtcgg ttaaatgcgc ttactgacat gagtttgtcc ggtatcaata ttgcggctaa
    13441 gtataaggag ctacaaaaat agattattga tatgaatcgg taatgatgcg actcattact
    13501 attccatttg taataaagta cgcagccctg ccgctttgtt gtcgatttcc tgccactctt
    13561 gctcggggtc ggaaccacgg acaatgcccg cgccagcata tagccgcacg acattgccgc
    13621 tgatttttgc tgaacgcagg gaaacgcaga attcgctttg ttgtaatgag agatagcccg
    13681 cagaaccggc gtaccattct cgggtgaacg gttcgtgacg ggcgataaac tgtcgcgcca
    13741 gatcgcgcgg taagccagca actgctgccg ttggctgcaa ctgatgtaaa cagatcacat
    13801 catccgcttt gttgagtgaa gtccagatac agcggcgaag atgctgcact ttacgcagac
    13861 gcagtacctg cggcggtaaa acatccagcg tctgggtatc ggcctgtaat cgttggcaga
    13921 tatcttccac caccagcatg ttctcgcgct ggtttttatc atccgccatc agccactctc
    13981 ctaactgctg cgcctgctta tcatcaggat tatttgctac tgttcccgcc agcgcttcag
    14041 tacgcagcgc tttgtcacgc cgccgccata accgttccgg tgaagagcca agaaaagcat
    14101 tttcgccatc aaaggccatg taaaaatggt agcaattcag attcagtcga cgactggcag
    14161 ccatcatcgc cgccgcgttg accggacttg cgaaatgcag gtcagttgcc cgagcgagca
    14221 ccactttgtc gagctcccct tcggcgatgg ttttcgttgc cagttcgatt aattgcgtcc
    14281 agcccgtttt gtccggccag tgttgttctc gcgtggtggt taaatgtaac ccaggcaagg
    14341 gcttgatact caccagtgtg gcgataaatt ctcttgcctg aatcgcatcg tgctgaaggg
    14401 agctttcgct gaatagcgtc agccgcagcg tggctttacc gccacagcgt cgccattcca
    14461 ggcgtggtaa aagtaaattg ccctgcgacg ggtcaaacgc attcagcccc caaatgcgta
    14521 agtcggcgtg ttccgggtgc tggcgaagaa aacgttgtgc ctggtccaac gacgtaaaac
    14581 gggtaatcgc gcccaggacg gcagcttctt catcaccatt acgttgttgc cagtagaatt
    14641 gcgggtacgt ttgctgactg gccagccagc tcaaggcatc aaaagcgtct ttgagaggga
    14701 aaggaatatc gataacccga atgccgggtg tcgccggaat ctcttgcgac aaatggcgca
    14761 gtagattttc cagcgccgta gtaagtgatt gcacgcggac ctctccctgt taaaagcctc
    14821 acattatacg gggtactaca aaaaaaatgc agtaccccgg tgtagggagg ttttaacggc
    14881 gtgccagcaa cagtcctagt accagcccga cggccgcgcc cacaccaatt ccttgccagg
    14941 gtttttcgtg gacgtagtca tcagcacgat aaacagcctg cttcgcccga tagtaataac
    15001 tgtctgaagc ctggctaacc cgttttttca catcatccag cgctttttct gcacgcgctt
    15061 tcagctcaac atatttctga tcggcgggat cgccagagga gcggagcacc tcttccagtg
    15121 tttcactaag cagcgtcagg tcgtcatcga tacgtgtatc accaaactga ttagacattc
    15181 tcgttctcca tttgcgtaaa acctgtcact aactatagac aagggttgtt gattacgcct
    15241 gaattacctc gcgtgccatc ccaatgtgtg ggataccatc ttcttcatag acctccgtca
    15301 cagggataaa accgaaactc tggtagaagt tttgcagatg cgcctgcgcc ccgaggtaaa
    15361 caggcttatc aggccagtga tgcgtgcagg tgtcgagggt tttactcatc agttgctgcc
    15421 ccactttttc gccacgcaac gcttcactga caatcacccg acctataacg accggctcaa
    15481 gatcatcatc acttttcaga atcctcgcat acgccaccag ttcatcattt ttccaaccga
    15541 gaatatggcg attatccccc gtcaaatcat caccatcgat atcctgataa gggcaattct
    15601 gctcgacaac aaacaccgcg caacgcagtt gtaataaggc ataaagctgg gaaaccgata
    15661 attcagagtg atgcagatct tgccattcaa tcataatgct ctccttttag atgtatgcct
    15721 tatactagcc ggaatatttt ttgaaacgtg atgaactcat ggaattaatt tttttaggta
    15781 cttcagccgg tgtgccaacc cgcacgcgca atgtcacggc aatattgctt aacctgcaac
    15841 atccgaccca gagcggactt tggttgtttg actgcggtga aggcacccag catcagctac
    15901 tgcataccgc ctttaaccct ggaaaactgg acaagatttt tatcagtcac cttcatggcg
    15961 atcatctttt tggtttaccc ggcttgctgt gcagtcgttc tatgtcaggc attatccaac
    16021 ccttaacgat ttatggtccg caaggtatcc gtgaatttgt ggaaaccgcg ctgcggatta
    16081 gcggctcatg gaccgattat ccgctggaaa ttgtcgaaat tggcgctggc gaaattctcg
    16141 atgatggcct gcgcaaagta accgcttatc cgctggaaca cccactggaa tgttatggct
    16201 atcgtattga agaacatgat aaaccgggtg cattaaatgc ccaggcatta aaagctgctg
    16261 gcgtgccgcc tggcccactg tttcaggaat taaaagcggg caaaacaatc acgctggaag
    16321 atggaaggca gattaacggc gcagattact tagctgctcc agtgccaggt aaagcgctcg
    16381 ctattttcgg cgataccggc ccctgcgatg ccgcacttga cctggctaaa ggtgtcgatg
    16441 tcatggtgca cgaagcgacg ctggatataa ccatggaagc caaagccaat agtcgcggcc
    16501 atagctctac acgccaggct gcgacactag cccgtgaggc tggagtcggt aagctaatca
    16561 ttacccacgt cagctcgcgc tatgatgaca aaggttgtca gcacctgtta cgtgaatgca
    16621 ggtcaatttt cccggcgact gaactagcga atgatttcac cgtgtttaac gtttaactct
    16681 gcttattatt aaaaacaggg cgaaacttgc cctgttatcg caacccgcgc tacatatatc
    16741 cacccacacg ggcatattga gcgatttttg atatttattc atctccttac gcactatatt
    16801 cagagcaata agttgcctct ttttatcttc attcagcaca acctttgcaa ctaaaaggag
    16861 cccatgatgg ttacagttgt cagcaattat tgtcaattat ctcaaaccca actcagtcag
    16921 acatttgcag aaaaatttac tgtgaccgag gaattactgc agtctttaaa aaaaacagcg
    16981 ttatccggag atgaagaaag tattgagtta ctgcataata ttgcgttagg ttatgatgaa
    17041 tttggaaaaa aagctgaaga tattctttac catattgtta gaaacccaac aaatgagacc
    17101 ctatcgatta tccgacttat taaaaatgcc tgtttaaaat tatataattt ggcacatacc
    17161 gcaaccaact cccaccttaa accaactggt ccggataatt cagatgttct cctgttcaaa
    17221 aagctattct ccccttcgaa attaatgaca attatcggtg atgaaattcc tcttatatct
    17281 gaaaaacagt cgctttcaaa ggtgctttta aatgatgaga ataatgaact gagtgatgat
    17341 acaaacttct gggataaaaa tcgtcaatta accacagatg aaatagcttg ctatcttcag
    17401 aagatcgccg ctaatgcaaa aaatactcaa gtcaattatc ctactggtct ctacgtcccc
    17461 tactccacca gaactcacct ggaagacgct ctcaatgaaa atattaagag cgatccatca
    17521 tggccgaatg aagtccagtt attccccata aatactgg
//
LOCUS       DAZFWP020000105        17485 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFWP020000105 DAZFWP020000000
VERSION     DAZFWP020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.105"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(393..1394)
                     /locus_tag="ACHK0Z_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             complement(393..1394)
                     /locus_tag="ACHK0Z_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4503326.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1502..1798
                     /locus_tag="ACHK0Z_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             1502..1798
                     /locus_tag="ACHK0Z_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4503327.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1921..2346)
                     /gene="arsC"
                     /locus_tag="ACHK0Z_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             complement(1921..2346)
                     /gene="arsC"
                     /locus_tag="ACHK0Z_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU4503328.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2359..3648)
                     /gene="arsB"
                     /locus_tag="ACHK0Z_RS16820"
                     /old_locus_tag="pgaptmp_003364"
     CDS             complement(2359..3648)
                     /gene="arsB"
                     /locus_tag="ACHK0Z_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU4503329.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3702..4055)
                     /gene="arsR"
                     /locus_tag="ACHK0Z_RS16825"
                     /old_locus_tag="pgaptmp_003365"
     CDS             complement(3702..4055)
                     /gene="arsR"
                     /locus_tag="ACHK0Z_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU4503330.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4564..4647
                     /gene="dinQ"
                     /locus_tag="ACHK0Z_RS16830"
                     /old_locus_tag="pgaptmp_003366"
     CDS             4564..4647
                     /gene="dinQ"
                     /locus_tag="ACHK0Z_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU4503331.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4701..6053)
                     /gene="gorA"
                     /locus_tag="ACHK0Z_RS16835"
                     /old_locus_tag="pgaptmp_003367"
     CDS             complement(4701..6053)
                     /gene="gorA"
                     /locus_tag="ACHK0Z_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU4503332.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6125..6967)
                     /gene="rlmJ"
                     /locus_tag="ACHK0Z_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             complement(6125..6967)
                     /gene="rlmJ"
                     /locus_tag="ACHK0Z_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU4503333.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7170..9212
                     /gene="prlC"
                     /locus_tag="ACHK0Z_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             7170..9212
                     /gene="prlC"
                     /locus_tag="ACHK0Z_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU4503334.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9218..9970
                     /gene="rsmJ"
                     /locus_tag="ACHK0Z_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             9218..9970
                     /gene="rsmJ"
                     /locus_tag="ACHK0Z_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU4503335.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10019..11488)
                     /gene="dtpB"
                     /locus_tag="ACHK0Z_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             complement(10019..11488)
                     /gene="dtpB"
                     /locus_tag="ACHK0Z_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU4503336.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11654..11707
                     /locus_tag="ACHK0Z_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             11654..11707
                     /locus_tag="ACHK0Z_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503337.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11806..12240)
                     /gene="uspA"
                     /locus_tag="ACHK0Z_RS16865"
                     /old_locus_tag="pgaptmp_003373"
     CDS             complement(11806..12240)
                     /gene="uspA"
                     /locus_tag="ACHK0Z_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU4503338.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12631..12966
                     /gene="uspB"
                     /locus_tag="ACHK0Z_RS16870"
                     /old_locus_tag="pgaptmp_003374"
     CDS             12631..12966
                     /gene="uspB"
                     /locus_tag="ACHK0Z_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU4503339.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13037..14536)
                     /gene="pitA"
                     /locus_tag="ACHK0Z_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             complement(13037..14536)
                     /gene="pitA"
                     /locus_tag="ACHK0Z_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU4503340.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14768..15970
                     /gene="yhiN"
                     /locus_tag="ACHK0Z_RS16880"
                     /old_locus_tag="pgaptmp_003376"
     CDS             14768..15970
                     /gene="yhiN"
                     /locus_tag="ACHK0Z_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU4503341.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16286..17338)
                     /gene="yhiM"
                     /locus_tag="ACHK0Z_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             complement(16286..17338)
                     /gene="yhiM"
                     /locus_tag="ACHK0Z_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU4503342.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4556 a         4389 c         4504 g         4036 t
ORIGIN      
        1 aaggaaaacg ttaacaacta tcaaaaagcg ctcattttaa aaagttatta tgttctccga
       61 tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt aataatgaac caggttaaag
      121 tgtatttaaa aatgtgttgt attagcgact gaaatctgcc ttttaaaaag gaggagggta
      181 ctgagacaaa gagtcattgc ttctccgata aaatgtcaga gaagcatgat tacgtggtta
      241 ttgtctgttg atgacttgtt gaccgagccg tcatcattct caatgcatta gttaaatcat
      301 ttgtgatttt taaggattat cctgtctatg attttctact tagaaggata tggcactaac
      361 tatgcatctt atagtttact gctggcatct atttaaaata gcaattgagt caaatagccc
      421 atcaagatag ccatacctaa tataaccgtc aggaatgcag ctatcatcgg cattctgaac
      481 attgatttaa gtaatatcac ttccgtcagg cttgcgccgg cactgccgat gattaatgcc
      541 attaatgctc ccagacccat tccttttgtc atcagaacag atgccagagg gataactgcc
      601 tcagcccgga tatacagcgg aataccgaca acggcgctta atgggatggc gaagggatta
      661 tctgctcctg catgagcggc aatccacgct gagggaataa agccataaat aaaagatcct
      721 attaatactc ctaacaaaag atatggcagt acatctttaa actgttgcaa cgcatctttg
      781 gtggctagtt ttatgggatt aatacttact acagctttgg tactgcaaca ggtaactacg
      841 gggttctcaa tggctttagc cgctggacta caacaactca cttttatggg cgtatatgtc
      901 gttcccggcg aagtttttgc tagagcacaa caatttgctg atgagctttt actggcaatg
      961 atatgacgtt caaaccccag agaatccagg ataatactgg caagaacgga gacgccggcg
     1021 gcgataatcg cgtacaacaa ggtgactttc caaccaaagg tcatccacat tagtccgacg
     1081 ataatgggat taagtaatgg tgaaacaaat aaaaaagtga gagtcggacc aaacccggct
     1141 ttcgctgaca acaatccacg taacatgggt attgtcgagc aactacagaa cggggtaacg
     1201 gctcccaaca gagcagccag gagatagcct ctcccttttc tggcccccat catctgctgg
     1261 attttatggt ctggcacctt ttgtcttatc aggctgacac cggcactaat cactataaac
     1321 agcaacgaaa gctcgacggc gagaaacaca aacatctctg cggcatcttg cagcatcgca
     1381 agccaactac tcatacgtaa cctcattgga taaatctata tttccagaat aatcgaaatg
     1441 aaattcagat caagatattt ctggtattat cgaaatatca accagacaaa gggggtgtca
     1501 tatgcagttg gaagaagttg ctaaagcgct gaaagagcta ggtcacccga cccgtttgtt
     1561 catattcaaa cacctggtca aagcggggga gcaaggtttg ccggtcggtg agttgcaaaa
     1621 gcagcttggg atacccggct ctacactcag tcaccatatt tcagcactgg tatcggtagg
     1681 gctggttaaa caaaaccgtg agagtcggac gctgatgtgt gtttcgcaat acgcaatgct
     1741 ggaagcgata attgaattct tacgcgagga gtgctgcgta aatagcaaaa ttgcctgatg
     1801 cgctacgcgc tacagatttc atgccatgca cggttttgta ggtcggataa gacgcgttag
     1861 cgtcgcatcc gacataaaca aagcgcactt taccaactat gggcagtgga tatcgccgct
     1921 ttatttcaac cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
     1981 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
     2041 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
     2101 gattaaccga tcgtcagtaa atttatcttc tgcaaggccc agttcctcat acggctcgac
     2161 gtttttacgc aacagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagctc
     2221 atcgcgcgtt ggcggtgttt ccagataata gataatggtc ggttctgtac cgctgttgcg
     2281 gatcatctcc agcgtattac gcgacgtacc gcaggccggg ttgtgataaa tggtaatgtt
     2341 gctcatatca atatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
     2401 cacaaaaagc accggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
     2461 agtcatattc ttctgcgaaa gtacgtgcag ccagagtagc gtagccaggc taccaattgg
     2521 ggtaattttc ggtcccaaat cgcagccaat cacattggca taaaccatcg cttctttgat
     2581 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
     2641 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
     2701 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
     2761 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     2821 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     2881 cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     2941 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     3001 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     3061 gagatgtaac atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     3121 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     3181 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     3241 cgtagtgcct ttactgaacc ctaaagccag cagcatggcg atgacaatcg gtgtcaaaat
     3301 aagcgccgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca ggacaatcca
     3361 ggtaaacagc aagcgaccac gaccattacc ccagcgtgag acgtgcagcg ccgcccattc
     3421 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcgataa acgcagccgt
     3481 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatggacca cgcccgtaac
     3541 taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     3601 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     3661 ataaaacaca tctgaaaatt catatgtgtt tagctaaatt tttaactgca aatgttctta
     3721 ctgtccccgg aacagttttg tcgagccagg ttgcggacaa tcgcctgaac cttttcctgt
     3781 tcacatcgcc aggcctcatc aataattttc gccgcccatg ctggaatatg cggtgataag
     3841 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     3901 gccaggtggc gggagatctt gggctgcgac tggtcgagag cagtgcagag atcgcagacg
     3961 cataactctc ccagttcgct gagcagtaaa acgatgccca gacgggtttc atcagcaaga
     4021 attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     4081 gtgtctctct tcgaagcgga taagtcaaaa acatatatga cttaacgaat gtgtaagtgc
     4141 aggggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga ggtctgaatc
     4201 aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggca
     4261 ctggctggag tgagaatgcc attatctaac gcatcaatgc taataccata ctgaaactat
     4321 tgcaaggaca tgctggtttt ataacctgaa tgtactgtat gattatccag ctagctctga
     4381 ggcattttca ctctggtaat gcgcataaac gctttcaaag tcctggtcag aagtacgggt
     4441 ggtgccgtta actgatgctc tggccggagt gagaatgcca ttatctaaca atagggcatg
     4501 cgccgtgaca ggcagtggat gagtaagcgg atgcattctc actccatcgc atggagaaaa
     4561 cgggtgattg ataaagcaat catcgttctt ggggcgttaa ttgcgctgct ggaactgatc
     4621 cgctttctgc ttcagcttct gaactgatag cggaaacgta attaagggct aagagcacac
     4681 tactcttagc cctttaacat ttaacgcatt gtcacgaact cttctgccgc cgttgggtga
     4741 atggcgacgg tattgtcgaa gtcttttttg gttgcaccca tcttcagcgc caccgcgaag
     4801 ccctgcaaca tttcgtccat accaaagcca atgccgtgaa taccgacaat cttctcttcc
     4861 gatccaacgc acaccagctt catgcggcac ggctggcggt gagtggtgac ggcggtatac
     4921 atcgcggtga aagaggattt atacactttc acctgatcgt cgccatactg ctcgcgcgcc
     4981 tgcggttccg ttaaaccaac agtaccaatc ggcggatggc tgaagaccac ggtcggaatg
     5041 ttgctgtaat ccagatgctc atccggcttg ttattaaaca ggcgttcaga gagacggcga
     5101 cccgctgcaa ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc caccgcgtaa
     5161 ataccttcaa tattggtgtt ttgatattta tcgacgacga tatagccttt ttcgttagtt
     5221 ttaacgccag cggcttccag gttgatgttg tcattggcag gctcgcgacc aatcgcccaa
     5281 atcaggcaat ccaccgtttc actgcgacca tcttccagct ccagcgtcag gctaccatcg
     5341 gcatttttca ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc ttcggcgttc
     5401 atcacttcga ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg cgcatgttta
     5461 cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag ctcaacggcg
     5521 atgtaacccg cgccaacaac cgccacgcgc tctggcaaag caggaagggc gaagaagcca
     5581 tcagaatcaa taccgtattc cacgcccgga atatccgggt ggctcggacg accgcctgtg
     5641 gcgatcagaa tatgatcggc cgtgatggtt tcgccgttta cctccagcgt tttggcatca
     5701 acgaagcggg caaagccttt gattacatca acgttatttt taccgagcac gttttcatag
     5761 gaagtatgaa tacggtcgat ataggcggta cggctggcga tcaacgtttc ccagttgaat
     5821 ttattgatag tggtatcaaa accataatcc gggccgtaca tatggatcgc ttcacggatt
     5881 tgcgccgcgt gccacatcac ttttttcggc acacagccga cgttcacgca ggtgccgccc
     5941 agctctttgg cttcaatcag tgcgcatttc tggccgtaca tagccgcgcg gttgatggag
     6001 gctataccgc cgctgccgcc gccgatggcg atgtaatcat agtgtttagt catgacagag
     6061 tgtccttatc gttgattacc gcgattgtag cgcgagacgt aataggtgcc agcaatggct
     6121 gcaattactc cggcacgatc cagcttacgg tggcgtgccc ggtgcctgcc ggaaccagtt
     6181 tgctgtgcag ccacggcagc acgttattca tctgttgttc cagtttccac ggcgggttaa
     6241 tcacaatcat gccggaagcg gtcatgccac ggcgatcgct gtctggcagt accgccagtt
     6301 caatttgcag aattttgcga ataccggtcg cttccagatc gtggatcatg cgcttaattt
     6361 gctgacgcag caccaccgga taccacagtg cgtaagtacc agtggcgaaa cgtttgtaac
     6421 cttctgctat cccgctgacc accgcctgat agtcggtttt catttcatac ggcgggtcga
     6481 taaggattaa accacggcgg gaaaccggcg gcagtttggc cttaagctgc tggaaaccat
     6541 cggctttttc gacgcgcgca cggctgtctt tctgaaattc agaacgcagc aacgggtaat
     6601 cgctcgggtg cagttcggtc agttgcaggc tgtcttgttc gcgcagtagc tggcgagcaa
     6661 tcaacggcga accggggtag taacgcaact gaccgctacg gttgaagtgt tttaccacat
     6721 tgatgtacgc ctccagttct gcgggcaaat cgtcctgctg ccagatacgg gcgatgcctt
     6781 cgagatattc gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc ccggcccctg
     6841 cgtgggtgtc gagatagaga aacggtttat ctttctcttt cagcgactcg atgatcaggc
     6901 tctgaacggt atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg tggcgataac
     6961 tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg gcgcacaaaa
     7021 gcgtatcagg acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac gtttctcatt
     7081 gaaattcact acacttaacc ccatgctaca cacattatgt aaagcgcctg ttgagcgctt
     7141 ccttaacctc tttaaccagg actgcgcgaa tgacgaatcc gttactgact ccctttgaat
     7201 tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc agccgtgact aaggcattga
     7261 acgactgccg cgaaaacgtg gagcgcgtag tagcgcaagg ggcaccgtac acctgggaaa
     7321 atctctgcca gccgttggcg gaagtggacg atgtgttggg gcgtatcttc tccccggtca
     7381 gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga agcgtacgaa caaaccctgc
     7441 cgctgctgtc ggaatacagc acctgggtag ggcaacatga agggctgtat aaagcgtacc
     7501 gcgacctgcg cgatggcgat cattacgcca cgctgaacac ggcgcagaag aaagcggttg
     7561 ataacgccct gcgtgatttt gaactctctg gcataggtct gccgaaagag aaacagcagc
     7621 gttacggcga aattgctacc cgtctttctg aactgggcaa ccagtacagc aacaacgtcc
     7681 tcgatgcgac aatgggctgg accaaactcg ttaccgacga agcggaactg gcggggatgc
     7741 cagaaagcgc gctggctgcg gcaaaagccc aggctgaagc gaaagaactt gaaggctacc
     7801 tgctgacgct ggatatccca agctacctgc cggtaatgac ctactgcgac aaccaggctc
     7861 tgcgtgaaga gatgtatcgc gcttacagca cccgcgcctc cgatcaaggt ccgaacgccg
     7921 gtaagtggga caacagcaag gtgatggaag agatcctcgc gctgcgtcac gaactggcgc
     7981 aactgctggg ctttgaaaac tacgccttta aatcccttgc cactaaaatg gcagaaaacc
     8041 cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg cgcgcgtccg caaggcgaaa
     8101 aagagctggc acaactgcgc gcctttgcca aagccgaatt tggcgtcgat gagttgcagc
     8161 cgtgggatat cgcgtactac agcgaaaaac aaaaacagca cctctacagc atcagtgacg
     8221 aacagctgcg tccgtacttc ccggaaaaca aagcggttaa cggtttgttt gaagtggtga
     8281 aacgtattta cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg cacccggatg
     8341 tacgtttctt cgaactgtat gacgagaaca acgaactgcg cggtagcttc tacctcgacc
     8401 tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta ggccagatgc
     8461 gcaaagctga cggttctctg caaaaaccgg tcgcgtatct gacctgtaac tttaaccgcc
     8521 cggtaaatgg taaaccggcg ctgtttaccc atgacgaagt gatcaccctg ttccacgagt
     8581 tcggtcatgg tctgcatcat atgctgaccc gcatcgaaac cgccggtgta tccgggatca
     8641 gtggggtgcc gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac tggtgctggg
     8701 agcctgaggc gctggcgttt atctctggtc actacgaaac cggcgaaccg ctgccgaaag
     8761 agttgctgga taaaatgctg gcggcgaaga actaccaggc ggcgctgttt attctgcgcc
     8821 agctggagtt cggcctgttc gatttccgcc tccatgccga gttccgcccg gatcagggag
     8881 cgaaaatcct cgaaactctg gcagaaatca agaaactggt tgccgtagta ccgtctccat
     8941 cctggggccg tttcccgcac gctttcagcc atattttcgc cggtggttat gccgcaggtt
     9001 actacagcta cctgtgggct gacgtactgg cggcagatgc tttctcgcgc tttgaggaag
     9061 agggcatttt caaccgtgaa accgggcagt cgttcctcga caacattctg agccgtggcg
     9121 gttcagagga gccgatggat ctgttcaaac gcttccgtgg tcgtgagccg cagctggatg
     9181 cgatgctgga gcattacggg atcaaaggtt agtttttgtg cagatttgct taattgatga
     9241 aacaggcacc ggagacggtg ccttatctgt tctggcggcc cgctgggggc tggagcacga
     9301 tgaagacaac ctgatggcgc tggtgttaac gccggaacat ctggaattgc gcaagcgtga
     9361 tgagccaaaa cttggcggca tctttgttga ttttgttggc ggagcgatgg cgcaccgacg
     9421 caaattcggc ggtggtcgcg gtgaggcggt ggcgaaagcg gtgggcatta aaggcgatta
     9481 tttgccggat gtggtggacg ccaccgcagg gctggggcgc gatgcctttg tgctggcttc
     9541 agtcggttgc cgcgtgcgga tgctggagcg taatccagtc gttgccgcct tgctcgacga
     9601 cggcctggcg cgtggttatg cggatgcgga aatcggcggc tggttgcagg agcggttgca
     9661 gttaattcac gcctccagcc tgacggcgct gactgatatt accccgcgcc cgcaggtggt
     9721 ttatctcgac ccgatgttcc cgcataagca gaaaagcgcg ctggtgaaaa aagagatgcg
     9781 tgtctttcag tcgctggtgg ggccggatct tgatgccgat ggattgctgg agcctgcgcg
     9841 gctgctggcg accaaacgcg tggtggtaaa gcgcccggac tacgcgccac cgctggcgaa
     9901 tgtcgccacg ccaaacgcgg tagtcactaa agggcatagg tttgatattt atgccggaac
     9961 gccggtgtaa aagtaaagca gggtagcgat aacgctaccc tgctttctgc aagaataatt
    10021 aatggctttc cggcgtcgca atcatgcgtt tcagccacgg caccatcaac agcatcacta
    10081 ctgcaacgcc cagcgtgacc agaccaatct taccaaacac gttggtatag acgggcaacg
    10141 tctcaagcgg atcggtaatg ttgtccggca ccgcggtaaa tgttgccaca tagccgccca
    10201 gcaagaacgc ggcagcctgc gtcaggaacc acatcccgag aataaagccc atcaaatgct
    10261 gcggcaccag ggcagcaatc atcgccagac ccagggcgct aataaacaat tcacccaggc
    10321 tctggaacaa gtaaaccagc acgataaacc acggcgatgt cagtccttgt gcatcagcaa
    10381 accacattcc cgcagctgcc gccgtcaaaa agcccagtga gcacataaac atgccgagag
    10441 taaatttcat cggcatcgag aggtctttgc ctttgttacc cagatgcgtg taaatgcctg
    10501 ccagtattgg gctggcgagt accacccaga acgggttaag cgcctggaag ctgaccgggt
    10561 tgatggaaaa gccgagaatt tcatgatgca cgttgttgat ggcaaagaag ttcagcgacg
    10621 ttggcatctg ggcgtagaga atgtaaaaca ccaccgcttc gagcatcagg acaaaggcga
    10681 caaacatttt attgcgcccg gttttatcca gcttgaatgc ctgacgaaag aagatgatgg
    10741 tgacgacgat ggagagaaca atcagcacca gattggcgac ttctacgttg tgcatcagcc
    10801 atgcacagac gaagatcatc accacgctgc caagtaacac gtacaacagt ttgctgaagc
    10861 tcattggccg gaagtcgggt tcagaaccaa tgtctttcac cattccacga caggcgatgt
    10921 aaaccagtaa tgcgataatt aaccccgccc cgcacaggtt gtaggtgact gaataaccga
    10981 atctatcagc gatcacaggg gccagcgata acgctatcaa cgagccgata ttgatcgaca
    11041 tatagaacag ggtgaatgcg ccatcaagcc gcggatcttt cggcggatag cacttcgaaa
    11101 gcaagctggc tgggttagct ttaaacaggc cgttaccgac agcgatagtc cccagggcga
    11161 tgaaaatcag gtcaggctta agtagcgaca tgccggtcat gaagtagcca atcgccagca
    11221 caagtgctcc gagaacaatg gtgcgtttgg tccccagcag gtggtcgccg acatagccgc
    11281 caatggaaat gaggccatag accagcgcag caaaagcacc aaaagtgaca aaagcctgct
    11341 cttgcgagaa tccaagctgt ttaacgaaga aaaccgccag tacgccctgc acgccgtagt
    11401 agccgaatcg ctcccataac tcgacaaaaa agatcatgaa aaatgggcga ggttgctgca
    11461 gcatccccat gggtgttgtt gtattcatat ttattaacct tccaatacca tcctgaaaag
    11521 tatgaacgcg taagggataa cgcgcgaaaa agtttacatc tgtcagagat tcttaatatt
    11581 gatgtggtga atagattaat acactattac aacagaaaat aaccagctga tcacactaat
    11641 gatggaaaat atgatggtgt taaatgttgt attggcagga gattttgatt gcgtacgcag
    11701 atcgtaggcc agataagggg tttacgctgc atcaggtaac aaggcgcgcc aaaaagcaaa
    11761 acgcccgcat atgcgggcgt tcaggaacac gtcgtggggg gatgattatt cttcttcgtc
    11821 gcgcagcgga acaatcagca tatcaacgtg aacggtgttg atcagctgac gtgcggaaga
    11881 cattagtttg ctccagaagt cctggtggtg accacaaacc accagatcca tatcgtattt
    11941 cttgattgca tcgaccagaa cctggcccag gtcgccgctg ccgctcaggg tttcagtgat
    12001 tgggtagcct gcattagtgg aaagctcggt cagcgcatga tgtgtctctt cagagatgcg
    12061 tttctgcata tcacccagat tcacatcaat aagcccggtg tataggtcag agtagtttac
    12121 atctacgtgg atcagagaaa ctttcgcatt gtaggggcga gccatagaga ctgctttctc
    12181 taccagaact ttgctttccg gggagagatc gaccgcgatg agaatgtgtt tataagccat
    12241 agtgttactc cttccataaa gttgtcgatg actggccagc tagcgtttct tgtgccgtta
    12301 cgtcacccgc gtcctgcgaa ctacgtgcgc ggggctaacc cagccgtaaa gattcagtgt
    12361 taagaccatc cttaccttat agcgacccgg atgatccgtc aatccgcctt gcttacccgt
    12421 tagataaaca aattttcgac atgaatatat tgatagtggt taaccttctg gaaaaaaaac
    12481 aacctgatct cctacactat ctatagagcc gctcggatgt tggtcaccac aaaagcggtt
    12541 tctccaggac ctggtgttaa ctgattggca gcgtatcggg agcggtagcc ccggggtgga
    12601 actccgcggg caggtcgccg gggaggaggt atgataagca ccgtcgcatt attttgggct
    12661 ttatgtgtcg tttgcattgt taacatggcg cgctatttct catcactacg cgcgttgtta
    12721 gtggtactgc gtaactgcga tccattgctc tatcaatatg ttgatggagg gggctttttt
    12781 acctcacatg gccaacccaa caaacaggtg cgtctcgttt ggtatatcta tgcccaacgt
    12841 tatcgcgatc atcacgatga tgaatttatt cgccgctgtg agcgggtgcg tcggcagttt
    12901 attctgacca gcgcattgtg tggtctggtg gtggtcagcc tgattgcatt gatgatttgg
    12961 cattaagcac ttcagcaata ataacgcata aaaaaagcgg gccagtaaac tgacccgctc
    13021 gttttggtgt gtacgattac aggaactgca aggagagcca gtacagtccg ccggaaagca
    13081 gtaccgcagc cggaagggta aacacccagg ccatcagaat actggttacg gttttacgct
    13141 gtaagccgcc gccatctacc accatcgtcc ccgcgacaga agaggagagt acgtgagtag
    13201 tggaaaccgg catcccggta taactcgcca ggccgataga cactgccgcc gtcatctggg
    13261 cagacatccc ctgagcgtag gtcatgcctt tcttaccgat tttctcaccg atagtcgttg
    13321 ccacacggcg ccagccaatc atcgtaccga tacctaacgc cagcgcgacc gccatgatga
    13381 tccacaccgg tgcatactcg atggtgctaa gcatgtcgga cttcagtttc ttcaacaggc
    13441 gctgatcgtc agcactcacg ccaggcatct tcaccacttt gtcgatagtg tcagaaacgc
    13501 acagcataat gcggcgcatc tggctacgtt gatcaagcga cagcttgtcg tagctttcca
    13561 catcggtggt caacatacct ttcaggcggt tgagcgcgtt aatggtattc gacggatggc
    13621 agtggaactc cgcaggttgc gttgcgccag cttccggagc cggtactaac tgatcagcac
    13681 cggtagcctg tttgagcagc gcaggatgct gctcaaagta agcttcgacg ttgttgatgg
    13741 catcacgggt acgggtgatt tcgtagccag tggcattcat gttcaccacg aagcctgctg
    13801 gcgcgacgcc aatcaatacc aacataacca gaccaatgcc tttctgacca tcgttcgcgc
    13861 cgtgcgaaaa cgccacgccg atagcggaaa ggatcagcgc gatacgcgtc cagaacggcg
    13921 gctttttctt gccgtctttc ttttcacgct ccgctggggt caggtggata cgggcgcgtt
    13981 tcttggtgcc gctccagtag cgacgcagca agaaaatcag accgccagca aacaccaggc
    14041 cgacaatagg ggaaacgatc agagaaccga aaatacttaa tactttcggg atattgagtg
    14101 catccaccac tgacgtcccg gtcatcaacg cattggttaa accaatcccg atgatcgcgc
    14161 caatcagcgt atgagagctg gatgcaggta aaccaaagta ccaggtaccc aggttccaga
    14221 taatcgccgc cagcaacata gagaacacca tggcaaggcc atgagacgat cccatattaa
    14281 gcagcagatc cgtcggcagc atatgcacaa tggcataggc aacactcaga ccacccagca
    14341 aaacacccaa aaagttgaac accgccgcca taaccacggc gagctgagaa cgcatcgcgc
    14401 gggtatagat aacggttgcc acggcgttgg ctgtgtcatg gaaaccattg atggcttcgt
    14461 agaacagcac aaaagccagt gcaagcaata ataacagccc ggtatgcaaa tccaggccag
    14521 caaacaaatg tagcatagga cgttacgcca ttttgaggac atgaacgcgg cgcattatca
    14581 gtgactttcg cggcgcgggc aaagtgaaat atagactttt tttgatttgc ctcctgtatg
    14641 gatttcactc aaaaaataat tatcttatat aattcaggca aatacttcct tttagtaata
    14701 ttgatgctgg tgcgaccact gaggaatctt tacaattcac gcccgttttt tctaagagga
    14761 gcgcaacgtg gaaaggtttg atgccattat tataggcgct ggtgcggcgg gtatgttctg
    14821 ttctgcgctg gcaggtcagg caggacgccg ggttctgctg atcgataatg gtaaaaaacc
    14881 agggcgcaaa atccttatgt ctggcggtgg gcgctgcaac tttaccaacc tttatgtcga
    14941 accaggcgct tatctgagcc agaatccgca tttttgtaag tctgcactcg cgcgttttac
    15001 ccagtgggat ttcattgatc tggtcaataa acacggcatc gcctggcacg agaaaacgtt
    15061 aggacaactc ttctgcgatg actccgcgca gcagattgtc gacatgctgg tggatgagtg
    15121 cgagaagggc aatgtaacct tcagattgcg tagcgaagtg ctgagtgtgg cgaaggatga
    15181 aacaggcttc acgcttgaac tgaacggcat gactgtcggt tgcgaaaagc tggtcatcgc
    15241 gaccggtggg ctgtcaatgc cggggctggg cgcgtcgccg tttggttata agattgccga
    15301 acaatttggc ctcaacgtgc tgccgacccg cgcgggtctg gtgccattca ctctgcataa
    15361 accgttgctc gaagagttac aggtgctggc gggcgtggcg gtgccttccg tgattaccgc
    15421 tgaaaacggc atcgttttcc gtgagaactt actcttcacc catcgcggct tgtctggacc
    15481 ggcggtgttg cagatttcca gctactggca accgggtgaa tttgtcagta tcaatctgct
    15541 accggatgtg gatctcgaaa ccttcctgaa tgagcagcgt aacgcacatc cgaatcagag
    15601 cctgaaaaac acactggcgg ttcatctacc gaagcggttg gttgaacgct tacagcaact
    15661 cgggcaaatc ccggatgttt cgctaaaaca gctcaacgtg cgtgaccaac aggcactgat
    15721 tagcacattg accgactggc gcgtacaacc caacggcact gaaggctatc gcactgccga
    15781 agtgacgctc ggcggcgtgg acaccaacga actctcttca cggacgatgg aagcgcgcaa
    15841 agtgcctggg ctgtacttca tcggcgaagt gatggacgtt accggctggc tggggggcta
    15901 taacttccag tgggcgtggt cgagtgcgtg ggcttgtgcg caggatttga ttgcagcgaa
    15961 gtcgtcctga cctgttacct aaagatgaaa attattagag ccgtattaaa aatgagctaa
    16021 ttttgatagt ggctatcttg tgattatttt ctaatgagcc cgtgaactga aaccctccag
    16081 gcttaatata aggtggaagg aaaggtgatt gaaaactcac ccagtggcca gaactttata
    16141 aaatatgaac aggttgaaaa aaacagtaac tttgttgttt ttttatcctt aaccactatg
    16201 cattaatgct gcgttatttc atgatgccta agaaaaacca gcgttacgca aatggtcaac
    16261 gctggtttta tccggtacgt tgcaattatt ttttagcaga acctgcttct aatattgaaa
    16321 cgattgagaa caacgtaaag cataccgctc ctaaaccgac caaaacccgc gaaggaataa
    16381 aatatcccat gtcggtctgc gccatttccg caagaaacga tgccaggaaa aggcaaaaca
    16441 ggcaggtgaa gacgggaatc atcggtattc tgttggctaa cgaacaggta cgtctccata
    16501 ccagtgccag tagccagact tttgagaata tgctgtaaca gatcattccg aggcaaataa
    16561 gaataatgcc gggagccagt cgggcgcttg catcggaact gactaagacg tatatcccct
    16621 gcagtaccgt gattgagccg agaaaaataa cccaataaca ccacagccag tgttctttag
    16681 ttgaaaacgt attacgtgtt tgatggacaa ttgtggcaac aaggccaatt agacaggcac
    16741 agattgcggt taacccaagc aatacgtgac ccgcgacata atgcggagtt atgtcagcac
    16801 tacgtaacag cgtaatagac caaatgaaac cgagcagggt gagtaaaacg ggaacggcaa
    16861 tgaggcaatt accgattaat gaagaataag cctgtaccgg cgttccatcg ctcttgctcc
    16921 ccgctgcatt tttgggaatg agcagaaagt gaccggatga cgctgccacc gtcgatacac
    16981 aggcggcaat cataccaacg ccgaaaataa catggccggc gacaaactcg tctgccatca
    17041 catcattgcc tgctaacagt gcccaacccc atatcatggt gataattgac cccgcatagc
    17101 caataatggg gaacaatgta ttgtaaaacg tattaacgcc gcgcgtgagc tgcgaaatga
    17161 taataaatgc ggtagtgaat aatgccaggc atatggccgc cagagaaatc agcacatgac
    17221 ccgcgacgaa atactcgctt tgccctgaac tttccagcac aaagccacct aacgcgatgc
    17281 aaattaatcc cataatcaaa gggattaatt tgaaaagcca cccgatatat atgttcactt
    17341 taaactcctc aggtatccct gctaataatg aaatgtgcat taatgataat ttcagcctgt
    17401 taatataacg acgagatatc attaaagaca aatgcatagc ataactatag cggggtttac
    17461 tgtgccgagt gccatttttt accgg
//
LOCUS       DAZFWP020000106        17084 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFWP020000106 DAZFWP020000000
VERSION     DAZFWP020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.106"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            78..347
                     /locus_tag="ACHK0Z_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             78..347
                     /locus_tag="ACHK0Z_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4503343.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHK0Z_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             316..1434
                     /locus_tag="ACHK0Z_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4503344.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(1511..1978)
                     /locus_tag="ACHK0Z_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             complement(1511..1978)
                     /locus_tag="ACHK0Z_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HGU4503345.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVELTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(1956..2159)
                     /locus_tag="ACHK0Z_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             complement(1956..2159)
                     /locus_tag="ACHK0Z_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503346.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            2468..3262
                     /gene="potC"
                     /locus_tag="ACHK0Z_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             2468..3262
                     /gene="potC"
                     /locus_tag="ACHK0Z_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU4503347.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            3259..4305
                     /gene="potD"
                     /locus_tag="ACHK0Z_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             3259..4305
                     /gene="potD"
                     /locus_tag="ACHK0Z_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU4503348.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            4363..4824
                     /locus_tag="ACHK0Z_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             4363..4824
                     /locus_tag="ACHK0Z_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HGU4503349.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            4821..5609
                     /gene="ycfZ"
                     /locus_tag="ACHK0Z_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             4821..5609
                     /gene="ycfZ"
                     /locus_tag="ACHK0Z_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4503350.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5748..6569)
                     /gene="cobB"
                     /locus_tag="ACHK0Z_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             complement(5748..6569)
                     /gene="cobB"
                     /locus_tag="ACHK0Z_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU4503351.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(6585..7496)
                     /gene="nagK"
                     /locus_tag="ACHK0Z_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             complement(6585..7496)
                     /gene="nagK"
                     /locus_tag="ACHK0Z_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU4503352.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(7525..8769)
                     /gene="lolE"
                     /locus_tag="ACHK0Z_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             complement(7525..8769)
                     /gene="lolE"
                     /locus_tag="ACHK0Z_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU4503353.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8769..9470)
                     /gene="lolD"
                     /locus_tag="ACHK0Z_RS16945"
                     /old_locus_tag="pgaptmp_003389"
     CDS             complement(8769..9470)
                     /gene="lolD"
                     /locus_tag="ACHK0Z_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU4503354.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(9463..10662)
                     /gene="lolC"
                     /locus_tag="ACHK0Z_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             complement(9463..10662)
                     /gene="lolC"
                     /locus_tag="ACHK0Z_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU4503355.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10924..11997
                     /gene="ycfT"
                     /locus_tag="ACHK0Z_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             10924..11997
                     /gene="ycfT"
                     /locus_tag="ACHK0Z_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4503356.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFTPPSLPAAVSYSR"
     gene            12125..15571
                     /gene="mfd"
                     /locus_tag="ACHK0Z_RS16960"
                     /old_locus_tag="pgaptmp_003392"
     CDS             12125..15571
                     /gene="mfd"
                     /locus_tag="ACHK0Z_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU4503357.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEATNLLPAHEFP
                     TDKAAIELFRSQWRDIFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQTDTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15715..16677
                     /gene="ldtC"
                     /locus_tag="ACHK0Z_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             15715..16677
                     /gene="ldtC"
                     /locus_tag="ACHK0Z_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU4503358.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16760..17017)
                     /gene="bhsA"
                     /locus_tag="ACHK0Z_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /gene_synonym="ycfR"
     CDS             complement(16760..17017)
                     /gene="bhsA"
                     /locus_tag="ACHK0Z_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU4503359.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4340 a         4380 c         4250 g         4114 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgga tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaagcccga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagcac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaactag agccaggctc
      901 accctggaag actggcaaaa aatttacaac gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccaaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctgggata
     1261 actccaccag caggaaaaac tccgccaaca tttcatgaac aacgctccct gtcagaacga
     1321 ctgtaccgcg cgcagggtat tgatacaaaa acattactgg gacataaagt ccaggcaacc
     1381 accgatcgtt ataacgatac tcgtggtcag gaatgggtta aattggttgt ttgacgaaat
     1441 aaatgcagct gaaaaatgtt gatattgctt tgctagtgta aaaaccccgg catcaaccgg
     1501 ggttcggaga ctaatcttga tcaggttcct gcttagaata atgttttcca acaataaatg
     1561 ccgtaaccaa tgcaacaaga tctattgaaa cgagagttcc ggcaagaact gtctccccca
     1621 tgacgccaaa gacagtggca gctaagatga tcaatattgc caaccaaaaa gcctttgttt
     1681 gaccatctct ggcaatatca atgctatcag ccactgtttt atggcgatga gcctgctctt
     1741 tttcggtcaa ctcaacaagt cgatttgcaa gcccaggcac cagttgatca tactttttaa
     1801 gcattgaagg tggcggaaca ggcccctgaa aatgctggca aacaatagcc cgcacctgcg
     1861 gactatccaa aaccctgttt aagacctctg gattttcgat tactcgagaa accagttcat
     1921 tatccttttg ttcttcacaa gtgagtttgg tgttctcact ttctttttga tctggcattg
     1981 ttcagctttt caacgttaac ggataccatt gctttttgga aatcgcctct gatagccgca
     2041 gcatcctgac gcaaagcgga aactggattc gctggcatgc catttgctga aacgaccgtc
     2101 gggctcaggg ctataaccga gccaatggct tgtaacaaag ccacactttt acgcttcata
     2161 gtcactcctg agttcaatgg gattcaaaac ggattcaaag tgaatcttga tagctaattc
     2221 taataccaca ttatgtggtt tttcaacacg aagaaatcac atcaaaaatc tcattcggaa
     2281 agcgatatgg gtaaacaaac tggaatactt ttctgtccta gtaagctcac ccatgtataa
     2341 aaatgctgta aaatgcgtgg tcctgttttg gggaaaagtt ttggagagat tttggagaaa
     2401 ggaaaaaatt gaataaattc aaaatcctga aagtgtcccg ccttatgaat aaaaaggtgg
     2461 aactcgaatg atcggtcgac tgcttcgcgg cggttttatg accgctatct acgcgtacct
     2521 gtatatccca atcattattt tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa
     2581 ctggcagggt tttaccacca aatggtatag cctgctgatg aacaacgaca gcctgttaca
     2641 ggcagcgcag cattcactaa caatggcggt gttttcggcg acgtttgcta cgcttatcgg
     2701 ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg
     2761 aatgctgttt gtggtgatga tgtcgccaga tattgtgatg gcgatttctc tgctggtgct
     2821 gtttatgctg ctgggtattc agcttggctt ctggtcgctg ctgttctcgc atatcacctt
     2881 ctgcctgccg tttgtggtgg tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat
     2941 gctggaagcg gcgaaagatc tcggtgccag tgaatttacc attctgcgaa aaatcattct
     3001 gccactggca atgcctgcgg tggcggcggg ctgggtgtta agctttaccc tgtcgatgga
     3061 cgatgtagta gtttcttcgt tcgtcaccgg accaagctat gaaattctgc cgttaaaaat
     3121 ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac gcgctggcaa ccatattact
     3181 ggtgctgtcg ctggtgatgg taattgccag ccagcttatt gctcgtgata aaacgaaagg
     3241 taacacaggg gacgttaaat gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg
     3301 gcactgggca tgagcgccgc tcacgccgat gacaacaata cgctgtattt ctacaactgg
     3361 accgagtacg tgccgccagg actgcttgaa cagttcacca aagaaaccgg tattaaggtt
     3421 atctattcga cttacgagtc gaacgaaacc atgtacgcca agttgaaaac gtacaaagac
     3481 ggtgcctacg acctggtggt tccttcaacc tattacgtcg ataaaatgcg taaagaaggg
     3541 atgatccaga agatcgacaa gtcgaagtta agcaatttca gcaacctcga tccagacatg
     3601 ctcaacaagc cgtttgaccc gaataacgac tactccattc cgtatatctg gggggcgacg
     3661 gcgattggcg ttaacggtga tgcggtagat ccgaaatccg ttaccagctg ggccgatctg
     3721 tggaagcctg agtacaaagg tagcctgctg ctgaccgacg atgcccgtga agtgttccag
     3781 atggcgctgc gcaaactggg ctactccggt aacactaccg atccgaaaga gattgaagct
     3841 gcatataacg agctgaaaaa actgatgcca aacgtcgcag cgtttaactc cgataacccg
     3901 gctaacccgt acatggaagg cgaagttaac ctcggcatga tctggaacgg ctctgctttc
     3961 gttgcacgcc aggcgggtac gccaattgac gtggtgtggc cgaaagaagg cggcattttc
     4021 tggatggaca gcctggcgat cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg
     4081 atcaacttcc tgctgcgccc ggatgtggca aaacaggttg ccgaaactat cggttatcca
     4141 acgccaaacc ttgcggcgcg taagctgtta agcccggaag tggcgaacga taaaacgctc
     4201 tacccggatg ctgaaaccat taaaaatggt gaatggcaga atgacgttgg cgcagccagc
     4261 agcatttatg aagagtatta tcagaagctg aaagcaggac gttaattcag caaacgggcg
     4321 gtggtattac cgcccgtttt ttagttagca aggatgtggc taatgagtca ggacagtaaa
     4381 gttttatccc gcgcttttct cggtatcggt ctgatattgc tcgttatttc tgcaatcatc
     4441 ttttattacc attttacttt taccaaaagc gcggttcata cagaaggaat cattgttgat
     4501 gccgtttggt acaacaatca ctcaaacgac gtcgatgata atggttcctg gtatccagtg
     4561 gttgcctttc gccctacgcc agattacacg ctaattttca attcgaatat cggtagtgat
     4621 ttttatgaag atagcgaagg tgacagagtc aatgtctatt atccgccagg gcatccagaa
     4681 caagccgaaa taaacaatcc ctgggttaat ttttttaaat ggggctttgt tggcatagca
     4741 ggcattattt tcggtagtgt cggtctgata atcacgctcc cctccaccaa aaaatctcgc
     4801 aggaaacgga agtcccgccc atgaaaaaat tcattatact gctcagttta ctgatactcc
     4861 tgcctctggt agcaaccagt aaaccattga ttccaataat gaagacactt tttacggatg
     4921 ttaccggtac tgtaccggat gctgaagaga tcgcacgtaa agccgagctt tttcgccaac
     4981 aaacgggaat tgcaccgttt attgtgattt tacccgacat taataatgaa gccagtctca
     5041 gacaaaatgg taaagcgatg ctggcgcatg cgtcatcttc attgagtaat gtaaaaggaa
     5101 gtgttctgtt actatttact acccgcgaac cacggttaat tatgatcacc aacggccagg
     5161 ttgaaagtgg tctggacgat aaacatctcg gccttctgat agaaaatcac acgctggctt
     5221 atttaaatgc agatctctgg tatcagggaa tcaataatgc attggctgtt ctacaagcac
     5281 agatattaaa acaaccgacg ccgccactga cgtattatcc gcatccagga caacagcatg
     5341 aaaatgctcc ccccggcagc accaacacat taggttttat cgcctgggct gcgacgttta
     5401 tcttattttc ctggatcttt tgctacacga ctcgttttat ttatgcgtta aaatttgcag
     5461 tggcgatgac tattgccaat atgggttatc aggctttatg tttgtatatc gataatagct
     5521 ttgcgattac cagaatatcg ccactctggg cagggctaat tggtgtctgt acgtttattg
     5581 ccgcattgtt attgacgagt aaacgataaa agaggccgga aaagtgcgcg tgtattatgc
     5641 cggatggggc gcgaacgcct tatccggccc acaaaacccg caaattcaat taattgcgtc
     5701 cccttgtagg cctgataagc gtagcgcatc aggcaatgct tcctgcttta taatcccttc
     5761 agcaactttt caacaaactc aggcaccacc tggcttgccg ggccgtaata tttctcggca
     5821 aattcattac cgacctgact tggttcgaga ttcagttcca cggtgtgcgc gccatgcagt
     5881 ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc cggaagtacc aatggcaatg
     5941 aaaatatcag ccatcgacaa cgccatataa atttcatcca tgccgagtgg catttcgcca
     6001 aaccaaacga cgtgcgggcg caagggtgcc gggaactggc aacaatggca tttatcttct
     6061 ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg aactttcagc
     6121 aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag gttgtctata
     6181 ttctgcgtta ccagcaaaaa gcgatcgccg agggcgtctt gcagtttagc cagcgcaaga
     6241 tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc gttataaaac
     6301 gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc ttcaacccga
     6361 tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc cgcagaaatc
     6421 cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac cactttatct
     6481 ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt acgaaaacga
     6541 cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc ggttagatgt
     6601 aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc aatgcgcgga
     6661 acacgagcta caggtaagag atgacgaggc agcctgtccg ccagttgcgt tgtgattgcc
     6721 gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat ggtcaggata
     6781 tttcccagac aaaccgctaa taaatccaga taacgctcaa cgtgcgccct tgcctgctca
     6841 tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg ataatagtgt
     6901 tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc atgctgacca
     6961 cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc aaccggcaga
     7021 cgcatatggc caaactcgcc ggtaatgtag cttttaccgg taatcggttt gccgttgaaa
     7081 atcagcccgc cgccaacgcc ggtgccgaga atcaacccca tcaccagtgg atattgagta
     7141 aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc aaggcgtaca
     7201 tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt taccgctggc agcggggaca
     7261 ttggcggcat acagcgtacc atcttctgtt tccggcattc ccggaatacc gatgccgaca
     7321 gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac tgcatctaaa
     7381 aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa ctgccgaccg
     7441 ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta atacattgcc
     7501 gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag ggtcaatatt
     7561 actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg ctgtgaccag
     7621 tacgtagaag acgtccagcc agtgcaattc cgatggcagg aaatcaataa aatagatatc
     7681 gctggagagg aactgatgac cgatcaactt ttcaatccac tcaataatcg gggtaagttg
     7741 cagtgaaacc accacaccga taatcacccc acacaggctg ccgaacagcc ctgccagcaa
     7801 tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg ttcttaatac
     7861 tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga tgttgaaaca
     7921 ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca tctggatatc
     7981 gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc tgttggtcac
     8041 ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt taagggcaat
     8101 acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg ggatcatggc
     8161 aaaactgtga tcgagttgac cactcaactg caaaataccg gcaacgtgca aacgcacacg
     8221 ttttggctgc atcagtttat gctcgggatt cgagttgggg atcataatcg acacccaatc
     8281 cccctgcttc actttcagcg catccgccac gcctttgccg atgataattt gctgttcgcc
     8341 cgctttaaaa ttgcgccacg catcgccctg gacaaacgag ggtaatgcgc tcagacgctg
     8401 ttcctgttgc gggttaacgc ccttcacctg gattgcgcga agattcgcgc cactttccac
     8461 cagcccggtg aaattgatat acggcgcagc ggcggcaata cccggcactt tttgcacgtg
     8521 atccagtgct tcctgccagt tagtccacgg ctgatccacc gcctcgattt caccatgcgg
     8581 caccaccgcc agaatacggt tgttcagttc gcgttcaaag ccgttcatcg cgcttaagcc
     8641 gacgatcaat accgccacgc cgagggcaat gccaatggta gaaatgacag agattagcga
     8701 caccatgccg ccgcgtcgcc gtccgcgact aaaacgcagg ccaattaata acgataaagg
     8761 catcgccatt actccgcccc catcaggctc agttccgccg tcagacgacc atcacgcatt
     8821 tctaactggc ggctcatacg tttcgccagt tgcaggtcgt gagtaaccac caggaaggcg
     8881 gtgccctgca agcgattcaa ttccccaagc aactggaaga tgctgtcggc gttacgcgca
     8941 tcgaggttac cggtaggttc atccgccagt accaggcgcg ggttattcac cagcgcacgg
     9001 gcaatcgcca cacgctggcg ttcgccgcca gaaagttcag atgggcggtg attcgcacga
     9061 tgctccagcc ccaccgcttt taacatctca agtgcacggc tgttgatttc agcgggcttt
     9121 ttcttgccaa taagcagcgg catagccacg ttttccaggg cggtaaaatc cggcagcagg
     9181 tggtgaaact gataaataaa gcccagcttc tggttgcgca gctctgcttt cgccgccgaa
     9241 gacagtttgc tcatcggttg accgttaaag atcacatcgc cggaggttgg cgtatccagc
     9301 ccgcccaaca agtgcagcaa ggtgctttta ccggaaccag agctaccgac gatcgccatc
     9361 atttcacctt cgccgacgct gaaactgaca ttgtgcagca catcggtttg cacactgcct
     9421 tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat aacgtaaagc
     9481 ctcagcgggt tgagtggcgg cagcgcgcca tgaagggtaa agcgtagaca gcagcgcgat
     9541 agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcga ccggcagcgc
     9601 cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc tggcaagcag
     9661 ggcgccaagc gccgctccga ggatcgcacc gataatcccg gcgctggccc cttgcaccat
     9721 aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg ctacttcgcc
     9781 ctgcttctcc attaccatca gccctagtga ggtaataatg ttaaacgccg caacggcgac
     9841 aatcaggctc agcagtaaac ccatcatatt tttttccatg cgtacggcct ggaacaactc
     9901 gcctttacga tcacgccagt cctgccattt gctgccttca ggcagttttt gctgacttaa
     9961 tgagtcgact ttcagcggct catccagcca caaacgccag ccggtaatat tgcctgccgg
    10021 ataacgcatc agacgcgagg catcctcaat attcaccagc atttcattgc catcgacttc
    10081 actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa tacgccccat
    10141 cggcgtgaac tggctggcag atggtaccat catgcggatt tgatcaccgc gattaacgcc
    10201 tagctgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct cgaggtcagt
    10261 ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcgccgggt cgataccgag
    10321 catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg
    10381 tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct gcgggttaag
    10441 agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt tgttttgcag
    10501 ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca ccccgagggt
    10561 aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg cacgcccacg
    10621 catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg tctggttgct
    10681 gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga actgtaagtg
    10741 atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc catgttttat
    10801 catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca gatacccatt
    10861 acacctcggg tcataacccg caggatcaac cgcatgtaaa ttaaaaaaga gcgtgttgtc
    10921 ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat ttgtctggtg
    10981 gtgatttatc actcggtcat taccttttat ccgcatctga ccacttttca gcatccgtta
    11041 tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc ctttcgtatg
    11101 ccggtttttt tctttatttc cggctatttg attcgccgct atatcgacag cgtaccgtgg
    11161 ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct ttggggcgtg
    11221 gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg cgatttaagc
    11281 aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg gatgatcacc
    11341 gccagcacca gcttgtggta tctgtatgcg ttaattgtct atttcgtggt atgtaaaatt
    11401 tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgttgagtgt ggcggttaat
    11461 ttcgttccca cgccgtggtg gggaatgaac agtgtgatcc gcaatttgcc ttattacagc
    11521 cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc gttgcgccgc
    11581 catctgctga tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt gtttactatc
    11641 tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta tcaatacgag
    11701 caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa caccattgct
    11761 atctacacca cccatcgcat tctggttgaa atattcagct taactctgct tgcgcaaatg
    11821 aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta tccctttgtc
    11881 agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca gcgtgcattc
    11941 agcgatctgt tgttcacccc gccttcactg cccgctgccg tcagttactc ccgctaagcc
    12001 atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat aatgaacagc
    12061 attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga atctgacaac
    12121 cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct
    12181 gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg aacgtcacgc
    12241 cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc atgatgaaat
    12301 cagtcagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc ttccctacga
    12361 cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt accagctacc
    12421 gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc
    12481 acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga
    12541 tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg tgatggagca
    12601 cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc
    12661 ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg acgtcgacag
    12721 ccagcgcacg ctggaggaag tagaagcgac caatctgctg cccgcgcacg aatttccgac
    12781 cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gatatcttcg aagtgaagcg
    12841 cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg ggatcgagta
    12901 ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt tccctgccaa
    12961 taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc agactgacac
    13021 gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc caccacaatc
    13081 gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc gggtgcagct
    13141 aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc agaaactgcc
    13201 agacctggcc gttcaggcac aacaaaaagc gccgctggat gcgctgcgta agttcctcga
    13261 gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg
    13321 tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg atgaagccag
    13381 cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg ataaggtgcg
    13441 taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc gccgtcgtca
    13501 ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg agctgcatat
    13561 tggtcagccg gtggtccatc tggagcacgg tgtcgggcgc tatgccggaa tgaccacgct
    13621 cgaagcgggc ggcattactg gcgagtattt gatgctcacc tatgccaacg acgccaaact
    13681 gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg cggaagaaaa
    13741 cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga
    13801 aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg ccgccaaaga
    13861 gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt
    13921 tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca tgtgtcagcc
    13981 gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat
    14041 gcgcgccgct ttcctggcag tagataacca caagcaggtg gcggtgttgg tgcctaccac
    14101 ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact ggccggtacg
    14161 tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt
    14221 ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa
    14281 gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa
    14341 agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg caacgccgat
    14401 cccacgcacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc
    14461 gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc tggtggtccg
    14521 ggaggcgatc ctgcgtgaaa ttttgcgcgg ggggcaggtt tattatctct acaatgatgt
    14581 ggaaaacatc cagaaagctg ccgaacggct ggcagaactg gtgcctgaag cacggattgc
    14641 catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg atttccatca
    14701 tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg acatcccgac
    14761 agccaacacc attatcattg aacgtgcgga tcacttcggt ctggcgcagc tgcaccagtt
    14821 acgcggtcgc gtcggacgtt cgcatcatca ggcatatgca tggttgctga caccgcatcc
    14881 aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc tggaagatct
    14941 cggggcaggt tttgcgctgg caacgcacga tctggagatt cgcggcgcgg gtgaactgct
    15001 tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata tggagttgct
    15061 ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag atctcaccag
    15121 ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt tcatccctga
    15181 cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc gccaaaacgg aaaacgaact
    15241 ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac
    15301 cctgctggat gttgcccgtc tgcgccagca agcgcagaaa ctggggatca ggaagctgga
    15361 aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta atccggcctg
    15421 gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc cgacgcgcct
    15481 gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac gccagtttat
    15541 gcgtgaactg gaagagaacg cgatcgctta atggtaaatc gggggcgttt ctgcgccccc
    15601 atctgcaaca tttacaaatt ctttgcactt ccctgcacta tccgacactg tcaccatcca
    15661 taattcaggc ttatctgttt attacaataa ccttatattt attatggatt tttggtgatg
    15721 atcaaaacgc gtttttctcg ctggctaacg ttttttacgt tcgccgctgc cgtggcgctg
    15781 gcgctaccgg caaaagccaa cacctggccg ctgccgccag cgggcagtcg tctggttggc
    15841 gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc tggaagccat cgccaaaaaa
    15901 tataacgtcg gctttctcgc tctgttacag gctaaccctg gcgttgatcc ttacgtaccg
    15961 cgcgcgggta gcgtgttaac gatcccgttg caaaccctac ttccagatgc gccgcgcgaa
    16021 ggcattgtga tcaatattgc ggagctgcgt ctctattact acccgccggg taaaaattcg
    16081 gtaaccgtgt atccaatagg tattggtcag ttaggtggtg acacgctgac accgacaatg
    16141 gtgaccaccg tttcagacaa acgtgcaaac ccaacctgga cgccaacggc aaacatccgc
    16201 gcccgttata aagcacaggg aattgagttg cctgcggtag tgccggctgg accggataac
    16261 ccaatgggcc atcatgcgat tcgtctggcg gcctatggcg gcgtttattt gcttcacggt
    16321 acgaacgccg atttcggcat tggcatgcgg gtaagttctg gctgtattcg tctgcgggat
    16381 gacgatatca aaacactctt tagccaggtc accccaggca ccaaagtgaa tatcatcaac
    16441 actccgataa aagtctctgc cgaaccaaac ggtgcgcgtc tggttgaagt acatcagccg
    16501 ctgtcagaga agattgatga cgatccgcag ctgctgccaa ttacgctgaa tagcgcaatg
    16561 caatcattta aagatgcagc acaaactgac gctgaagtga tgcaacatgt gatggatgtc
    16621 cgttccggga tgccggtgga tgtccgccgt catcaagtga gcccacaaac tctgtaaatc
    16681 ctggataaaa aaaatccccg cgacacggca tgtcgcgggg aaaaaatcag cagtagcagg
    16741 cattaatgag ggttaatgct tatttataaa ttactgctgt tccatggagg gtattcggac
    16801 cggttacaga agtaatacgg aaagattttg cgcccatctc atccgctttt tgcgccagct
    16861 gctcttccag cgatcccaga tttgtccccg cgttagcact gattgtaccg actttttgtt
    16921 ggccttctgg cgttgactga acttcgacag ccgcaaagct ggcaaatgac atggagctta
    16981 aaatcgccgc agcgatgagg gtttttacgt ttttcataat agtggcctta tgcagatgaa
    17041 tgacgtcgca agcaattact taacgatcga taactaaatg atag
//
LOCUS       DAZFWP020000107        16794 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFWP020000107 DAZFWP020000000
VERSION     DAZFWP020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.107"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..92)
                     /locus_tag="ACHK0Z_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             complement(<1..92)
                     /locus_tag="ACHK0Z_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503360.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERM"
     gene            complement(220..2181)
                     /gene="topB"
                     /locus_tag="ACHK0Z_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             complement(220..2181)
                     /gene="topB"
                     /locus_tag="ACHK0Z_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU4503361.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2186..3229)
                     /gene="selD"
                     /locus_tag="ACHK0Z_RS16985"
                     /old_locus_tag="pgaptmp_003397"
     CDS             complement(2186..3229)
                     /gene="selD"
                     /locus_tag="ACHK0Z_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU4503362.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3346..3897)
                     /gene="ydjA"
                     /locus_tag="ACHK0Z_RS16990"
                     /old_locus_tag="pgaptmp_003398"
     CDS             complement(3346..3897)
                     /gene="ydjA"
                     /locus_tag="ACHK0Z_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU4503363.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4058..5914
                     /gene="sppA"
                     /locus_tag="ACHK0Z_RS16995"
                     /old_locus_tag="pgaptmp_003399"
     CDS             4058..5914
                     /gene="sppA"
                     /locus_tag="ACHK0Z_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU4503364.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6081..7097
                     /gene="ansA"
                     /locus_tag="ACHK0Z_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             6081..7097
                     /gene="ansA"
                     /locus_tag="ACHK0Z_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU4503365.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETICKAMSQNLRGEL
                     TPDD"
     gene            7108..7749
                     /gene="pncA"
                     /locus_tag="ACHK0Z_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             7108..7749
                     /gene="pncA"
                     /locus_tag="ACHK0Z_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU4503366.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7910..8182)
                     /gene="yeaC"
                     /locus_tag="ACHK0Z_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             complement(7910..8182)
                     /gene="yeaC"
                     /locus_tag="ACHK0Z_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU4503367.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8224..8637)
                     /gene="msrB"
                     /locus_tag="ACHK0Z_RS17015"
                     /old_locus_tag="pgaptmp_003403"
     CDS             complement(8224..8637)
                     /gene="msrB"
                     /locus_tag="ACHK0Z_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU4503368.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8979..9974
                     /gene="gapA"
                     /locus_tag="ACHK0Z_RS17020"
                     /old_locus_tag="pgaptmp_003404"
     CDS             8979..9974
                     /gene="gapA"
                     /locus_tag="ACHK0Z_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4503369.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10058..10942
                     /gene="yeaD"
                     /locus_tag="ACHK0Z_RS17025"
                     /old_locus_tag="pgaptmp_003405"
     CDS             10058..10942
                     /gene="yeaD"
                     /locus_tag="ACHK0Z_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU4503370.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10993..11847)
                     /gene="yeaE"
                     /locus_tag="ACHK0Z_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             complement(10993..11847)
                     /gene="yeaE"
                     /locus_tag="ACHK0Z_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU4503371.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11937..12683)
                     /gene="mipA"
                     /locus_tag="ACHK0Z_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             complement(11937..12683)
                     /gene="mipA"
                     /locus_tag="ACHK0Z_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU4503372.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13119..15053
                     /gene="yeaG"
                     /locus_tag="ACHK0Z_RS17040"
                     /old_locus_tag="pgaptmp_003408"
     CDS             13119..15053
                     /gene="yeaG"
                     /locus_tag="ACHK0Z_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU4503373.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWSSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15166..16449
                     /gene="yeaH"
                     /locus_tag="ACHK0Z_RS17045"
                     /old_locus_tag="pgaptmp_003409"
     CDS             15166..16449
                     /gene="yeaH"
                     /locus_tag="ACHK0Z_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU4503374.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            16596..>16794
                     /locus_tag="ACHK0Z_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             16596..>16794
                     /locus_tag="ACHK0Z_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4503375.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLT"
BASE COUNT         4166 a         4322 c         4096 g         4210 t
ORIGIN      
        1 atcatccgtt caaaattact attaaatatt tcccagccat taaaagaata cttctcgctc
       61 ccaggatggt tttgtaataa aacttttttc atcacattcc ctgttattac atactttaga
      121 ggcgataatt ttatccatgc aaaaaaaata tccaacgaat tttcttgatc cgctggatat
      181 ttcagaatat gactcgaata gcacgaaaga ttcgctcgct tacgctatcg ccccgcttcc
      241 ggcttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag cctttttctt
      301 atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca ccggcgtacg
      361 tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa agtcctgata
      421 gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg tcatgtccgg
      481 tcgcgtagcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg tggagtggat
      541 atagcgccct tttttggtga ggaaaccacg cttgaacaac agttcaataa tcccggcacg
      601 cgttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat ctttgtcctg
      661 cacaaagcgc gcaatcccgg tcatcgccga aagcagtgtt gcatcggtaa aatggcgcgg
      721 cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat cgcctttcgc
      781 caccacaggc aatggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc ctaacagcgc
      841 gcgccaacct gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt tggcaatgtc
      901 cagttcgata acacacttgc ggaacactgc atccgggcag aactgcatca gatactgacg
      961 ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag aactccttgc
     1021 ggtcggaatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc ggttgcgtat
     1081 atctggatct accactggct gcggcaacag atctggtgca tgaacgctga tggcattcat
     1141 caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg aacgcggata
     1201 agtgattaac ttgtgtgttt cgtacagctt ctggcagata tcaagcacgt tctgcgcgct
     1261 cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaacg gcagcggcgc
     1321 ggattctgat tcccgtttat cgttatagct ggtgacaata gccggttgac cactaatgcg
     1381 gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat cctggtacgg
     1441 ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag gtgtcacgat
     1501 atgcgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt catcgcggcg
     1561 caccaccagc ccgagcacgg gtgtctgcac gcgtcccacg gaaagtacgc cctgataacc
     1621 ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca gccagtcggc
     1681 acgcgctcgc gccagcgcag aaacgcacaa tggcacaaac tcactgttgg aacgaagacg
     1741 gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac gctgcacctg
     1801 ctggcgcttt tccggtgcca gttgcagata gtccagcact tcatccacca gcaattgccc
     1861 ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcagga agcgtttgat
     1921 gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt ccgggacaat
     1981 cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg gctgcgcctg
     2041 ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact cgataaagcc
     2101 atcgccttta cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca gactcggttt
     2161 ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga ccgccgcgcg
     2221 ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct gtagttttaa
     2281 cctcattttc tgcttccggc atgaccgcca gcagcaaacc gccagaagtt tgcggatcgc
     2341 acagcagatc gcgcacttca cgcggcattt cacccatcag atgaccgtag ctggcaaagt
     2401 tacgttcggt gccgccaggc actgcgccca acttaatgta ctcttcaacc cccggcaatt
     2461 ttgggatcgc gtcatagtca acgcgcgcct gcacacctgc gccctgacac atttcgctca
     2521 aatggcccag cagaccaaaa ccagtaacgt cggtcatcgc ttttacgcct tcgatgttgg
     2581 caaaggacgc acctgcgatg ttcatccggc acatcacttc cgtcgccagt ccctgatgtt
     2641 ctggtttcaa cagtgatttt ttctcagccg tggtaagaac gccgatcccc agcggtttcg
     2701 tcaggaacag tttgcatccg gcttgtgcgg tactgttttt cttcacccgc tcggtcggta
     2761 cgatccccgt taccgccaga ccaaaaatcg gctccggcgc atcgatggag tgacccccag
     2821 ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggttact tcgcgggcaa
     2881 tttctgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata atcggtttgc
     2941 cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca aaatcgaaag
     3001 gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg ccattgccca
     3061 gatcgtacac cgccgcatcg tcgcgggtct cattacccac aagcaaattc ggatcaacaa
     3121 acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt ttacagccgc
     3181 aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg gacatctcct
     3241 gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga gagtgtctga
     3301 attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga aataagttac
     3361 aaacggcgtc gggtccggga cgttaatcga cgtagatgct ttcaactgcg gcgtgccgag
     3421 gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac gcactaccgg
     3481 actttcagtt aatgcgccac tgcgccagat gccgccaaat ccttgggcaa cggctgccat
     3541 ttgcatcgcc atgaccgcgc atccggcaga catttcctgt tcccatcgcg ggactttatg
     3601 attttcttcg catttcgcca ccaccgtgat gatgagcggt gcgcggaacg gcgcattacg
     3661 ggctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt ccagtacggc
     3721 gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa tgccacggtt gcatggactt
     3781 atggtccggc gcacgcatac ccgcacgcag gatgttttgc agttgttcac ccgttggcgc
     3841 gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg catccatttg
     3901 attaactcct gtcgtgatat ttattcacaa aattaacacg agagtggatt ttgttacagc
     3961 acagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc acatctattg
     4021 cgcctgtgac aggtgtgacc ttaagttggg agaatacatg cgaacccttt ggcgatttat
     4081 tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa tggtacttaa
     4141 cctgttcttt attttcctcg tactggttgg tgtggggatc tggatgcagg tcagtggtgg
     4201 tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg gacatttctg gtgtgatcgt
     4261 cgataaaccc gacagttctc agcggtttag taaattaagc cgccagctgc ttggtgccag
     4321 ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc gccaggcgaa
     4381 ggacgaccgc aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag gcggcgacca
     4441 gccgtctatg cagtacatcg gcaaagccct gaaagagttt cgtgacagcg ggaaaccggt
     4501 ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt tcgccaataa
     4561 aatttggctg tcaccgcaag gcgtggttga tctgcacggt tttgctacca acggtctgta
     4621 ctacaaatcg ttgctggata agctgaaagt ttccacccat gtgttccgcg tgggtacgta
     4681 taaatctgcc gttgaaccgt ttattcgtga tgatatgtca ccggcagccc gcgaagctga
     4741 cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg ctaaccggca
     4801 gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt taaccaaaac
     4861 cggtggcgat accgcgaaat atgcactgga aaacaagctg gtcgatgcac tggcatcgag
     4921 tgccgaaatc gaaaaagctc tgaccaaaga gttcggctgg agtaagactg ataaaaatta
     4981 tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg gtgacagcat
     5041 cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg ggaatgttgg
     5101 cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag tgaaagcgat
     5161 tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga ttcgcgctga
     5221 actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg gcatggcggc
     5281 atctggtggt tactggattt ccacgccagc taattacatt gtggctaacc ccagcaccct
     5341 gaccggttct atcggtatct tcggcgtgat caccaccgta gaaaatagtc tggattcgat
     5401 tggtgttcat actgatggtg tctcaacttc accgctggcg gatgtttcta tcaccagggc
     5461 actgccgccg gaagcgcagc agatgatgca attaagcatt gagaatggct ataaacgctt
     5521 tatcacgctg gttgctgatg cgcgtcattc gacgccggag caaattgata aaatcgccca
     5581 gggccacgtc tggaccggtc aggatgcaaa agctaacggg ctggtcgata gtctcgggga
     5641 tttcgatgat gcggttgcca aagcagcaga gctggcaaaa gtgaaacagt ggcatctgga
     5701 atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt ctggttctgt
     5761 ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgcttg cctcggtagc
     5821 ctctaccgtt aaaagtgaaa gcgacaagct ggccgcgttt aacgacccac aaaaccgtta
     5881 tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg tactgagtgg ccgacagatc
     5941 gtcggccaca ttatttttta cctcgacgaa tcctcttccc gctgtctcga cccatatttc
     6001 cttatccgca tagtatcagg tgcgctcctc ctgcctcacg tatatacttt tgctctttcg
     6061 atatcattca tatcaatatc atgcaaaaga aatcaattta cgtcgcctac acgggcggga
     6121 ccatcgggat gcagcgttcc gagcagggtt atattccggt gtcaggtcat ctacaacgcc
     6181 aactggcgct gatgccggaa ttccatcgcc cggagatgcc agatttcacc attcatgaat
     6241 atacgccgtt gatggattct tctgatatga cgccggaaga ctggcagcat attgctgaag
     6301 atattaaagc gcactatgac gactatgatg gttttgtcat tctacacggt accgacacga
     6361 tggcgtatac cgcctctgcg ctgtcgttca tgctcgagaa tctcggtaaa ccggtcattg
     6421 tgacagggtc acaaatcccg ctggctgagt tacgctctga cggacaaatt aatctgctga
     6481 atgcgttgta cgttgcggcg aattatccga tcaacgaagt aacgctcttt ttcaataacc
     6541 gattgtatcg cggcaaccgc actaccaaag cccatgccga tggttttgat gcgtttgcct
     6601 ctccaaacct tcctccgtta ctggaagctg gtatccatat tcgtcgtttg aatacgccac
     6661 ccgccccgca cggtgaaggg gaattaatcg ttcatccaat cacgccacaa ccaattggcg
     6721 tagtgacgat ttatccgggg atttctgctg atgtcgtgcg caattttctg cgccaaccgg
     6781 tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc gccacaaaac aaagccttcc
     6841 tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt ggttaacctg acacaatgta
     6901 tgtccggtaa agtgaacatg ggtggttatg ccaccggtaa cgccctcgcc catgccggcg
     6961 taattggcgg tgcagatatg actgtagaag ctacgctaac caaactgcat tacctgctga
     7021 gccaggaact ggatactgaa accatttgca aggccatgag ccaaaacctg cgcggcgaac
     7081 tgacgccgga tgattaagga gactgcaatg ccccctcgcg ccctgttact ggtcgattta
     7141 caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg aaggtgacag tacggtggat
     7201 gtcgctaacc gccttattga ctggtgccag tcgcgcggtg aagcggttat cgccagtcag
     7261 gactggcacc cggcgaatca cggcagtttt gccagtcagc actgtgtaga gccttatatg
     7321 ccaggtcaac tcgacggttt gccacaaacc ttctggccag atcactgtgt gcagaacagt
     7381 gaaggcgcac aattacatcc gttactgaac caaaaagcga tcgcagcggt gttccataaa
     7441 ggcgaaaatc ctttagttga cagttacagt gccttttttg ataacggccg tcggcagaaa
     7501 acctctctcg atgactggtt acgcgatcat gaaatcgatg aattgatcgt tatgggcctg
     7561 gctactgact attgcgtgaa gtttaccgtg ctggacgcgt tacagttagg ttataaagta
     7621 aacgtgatta ccgatggttg tcgtggcgtg aatatccagc cccaggacag tgcgcacgcg
     7681 tttatggaga tgtcagcagc tggggcaacg ctatatacgc tggcagactg ggaagagacg
     7741 caggggtaat attagttgaa ttcatctctg gcctacattt cctcaatgac attgtaggcc
     7801 agataatact tgtcagtgtt gcatttaacc tacttgcagc taaaacacga cgtcctaaca
     7861 agtatatttg ccctccctta ataacaggct taaatattag ataactcatt cacttaaaca
     7921 tagcaatagg ctttgctgag ataccaaaat cttcttttaa ctgctgtttg ctcttcatga
     7981 ccatctgacc atttgtgtca attgtcatat gctgtgcttc ggtattatgg cgggcttgcc
     8041 acagcatcac cagttgcagg caattctctt tttgttcctc ggtcaacgca acgccatcag
     8101 gccatttccc cagttcaacg gcggtcgaca aacgctggta tacctcaggc gtcatgctgt
     8161 tgataatgtc atcaagattc ataatttttc cgctcctgtg gaataagttg ctgaatcgtt
     8221 ttttcaaccg ttgatttctt cgccgttttc gccatcggta aagcgtaaag aggcagagtt
     8281 aacacaataa cgttcgcccg ttggctgcgg cccgtcaggg aagacatgcc ccagatgggc
     8341 atcacagtta ccgcaacgaa tttctatgcg ctgcattcca tgtgacaagt ctttgatata
     8401 acgaatggat tcttcactca ccggttcgta gaaactgggc caaccacagc cggaatcata
     8461 cttggtttgg gaatgaaaca gcggggcatc gcagatcaaa cagtgatata cgccgtcacg
     8521 cttgttatgc agtaaacgac ccgtaaatgg cggctctgtc ccatgattct gcgtcacgta
     8581 aaactgcatc tcggacaaat tttttttcag ttcttctgcc gaaggtttat tagccatttg
     8641 ctcacatctc actttaatcg tgctcacatt acgtgactga ttctaacaaa acattaacac
     8701 caactggcaa aattttgtcc taaacttgat ctcgacgaaa tggctgcacc taaatcgtga
     8761 tgaaaatcac atttttatcg taattgccct ttaaaattcg gggcgccgac cccatgtggt
     8821 ctcaagccca aaggaagagt gaggcgagtc agtcgcgtaa tgcttaggca caggattgat
     8881 ttgtcgcaat gattgacacg attccgcttg acgctgcgta aggtttttgt aattttacag
     8941 gcaacctttt attcactaac aaatagctgg tggaatatat gactatcaaa gtaggtatca
     9001 acggttttgg ccgtatcggt cgcattgttt tccgtgctgc tcagaaacgt tctgacatcg
     9061 agatcgttgc aatcaacgac ctgttagacg ctgattacat ggcatacatg ctgaaatatg
     9121 actccactca cggccgtttc gacggtaccg ttgaagtgaa agacggtcat ctgatcgtta
     9181 acggtaaaaa aatccgtgtt accgctgaac gtgatccggc taacctgaaa tgggacgaag
     9241 ttggtgttga cgttgtcgct gaagcaactg gtctgttcct gactgacgaa actgctcgta
     9301 aacacatcac cgctggtgcg aagaaagtgg ttatgactgg tccgtctaaa gacaacactc
     9361 cgatgttcgt taaaggcgct aacttcgaca aatatgctgg ccaggacatc gtttccaacg
     9421 cttcctgcac caccaactgc ctggctccgc tggctaaagt tatcaacgat aacttcggca
     9481 tcatcgaagg tctgatgacc accgttcacg ctactaccgc tactcagaaa accgttgatg
     9541 gcccgtctca caaagactgg cgcggcggcc gcggcgcttc ccagaacatc atcccgtcct
     9601 ctaccggtgc tgctaaagct gtaggtaaag tactgccaga actgaatggc aaactgactg
     9661 gtatggcgtt ccgcgttccg accccgaacg tatccgtagt tgacctgacc gttcgtctgg
     9721 aaaaagctgc aacttacgag cagatcaaag ctgccgttaa agctgctgct gaaggcgaaa
     9781 tgaaaggcgt tctgggctac accgaagatg acgtagtatc taccgatttc aacggcgaag
     9841 tttgcacttc cgtgttcgat gctaaagctg gtatcgctct gaacgacaac ttcgtgaaac
     9901 tggtatcctg gtacgacaac gaaaccggtt actccaacaa agttctggac ctgatcgctc
     9961 acatctccaa ataagttgag atgacactgt gatctaaaaa gagcgacttc ggtcgctctt
    10021 ttttttacct gataaaatga agttaaagga ctgcgtcatg attaagaaaa tttttgccct
    10081 tccggtcatc gaacaaatct cccctgtcct ctcccgtcgt aaactggatg aactggacct
    10141 cattgtggtc gatcatcccc aggtaaaagc ctcttttgca ttacagggcg cacaccttct
    10201 ctcgtggaaa cctgcgggtg aagaagaagt tctgtggttg agcaacaaca caccgttcaa
    10261 aaatggcgtc gctattcgcg gtggcgtacc ggtttgctgg ccgtggtttg gtccggcggc
    10321 acaacaaggt ctgcctgcgc acggttttgc ccgcaacctg ccgtggacgc tgaaatcaca
    10381 tcgtgaagat gctgatggcg tagcgctgac ttttgaattg acgcaaagcg aagagacgaa
    10441 aaaattctgg ccgcacgact ttacgctgtt agcgcatttc cgcgtgggta aaacctgtga
    10501 aatcgatctt gaatcacatg gcgaatttga aaccacctct gccctgcata cctactttaa
    10561 cgtgggtgat atcgctaagg taagcgtcag tgggctgggc gatcgcttca ttgataaagt
    10621 aaatgacgcg aaagaagatg tactgaccga cggtattcag accttccctg accgtaccga
    10681 tcgcgtgtat ctgaatccgc aagattgcag cgtgattaat gatgaagcgc tgaatcgtat
    10741 tatcgccgta ggccaccagc atcatctgaa cgttgtcggc tggaacccgg gaccggcgct
    10801 ttcagttagc atgggcgaca tgccggatga tggctacaaa acatttgttt gtgtagaaac
    10861 ggcttacgct tcagaaacgc aaaaagtgac caaagagaaa cctgcacatc tggcgcaatc
    10921 cattcgcgtt gcgaaacgtt aatttgcgtt aatgttgtgt gccgggtgcg atgcatccgg
    10981 cacgtatcaa catcacacca tatccagcgc agtttttcct tttggtgccg gatacgcttt
    11041 atccagcatg attaattccg ctgacgaaag ttcgacctca agcacagccg cattctgttg
    11101 gacatgggca atcgtggccg cttttggaat cgccatcaca ccctgatggc tgattaccca
    11161 cgccaacaat acttgtgccg cgctgatatt gtgagcatgt gcaatttcgt tgactaccgc
    11221 gttttttaac agtccattgc gcaaccgccc ggcctgggct aatggactgt aagccattac
    11281 cggcatctgc tgttgctggc accaggggag tagatcgtac tcaatccctc gtgaaccgag
    11341 atggtaaagc acctgattag tggcacactg atttcccccc ggcagctgcc agagttcctg
    11401 catatcagca taatcaaggt tagaaacgcc ccagcggcgg atttttccct gggcgatcaa
    11461 tttttccatc gctgccacag tctcttcaaa agcgaaactg ccagaccagt gtaataagta
    11521 gagatcgaga taatcagtat tgagacggcg taaactggct tcgcatgcat ttatcgcttt
    11581 ttgcccgcca gcattccacg gatagacttt agagacgaga aagacgttat cgcgcagatc
    11641 ggttaatgct tccccaacca ccttttcggc accgccatcg gcatacattt cggcggtatc
    11701 aatgagggtt aaaccgagct caatgcccgc gcgtagtgca gcaacttctg ttttgcgctg
    11761 actggcatct tcgcccatat accatgttcc ctgccctatg gctggcagtg agacatcgcc
    11821 actaaattga atcatttttt gttgcattgt ttcctcccag gtattgcacc accgtaatgc
    11881 aaaacagggc gtgacgccct gtttttatgc acaaaatgcc ctggaaagat gcattatcag
    11941 aatttgtagg tgatcccggt agaaatcagg ccagtccagg atttatccac catcgggctg
    12001 tcagtaactt catcagacag acgggtatag cgcgcggtac cgtaaacact ccagtcgccg
    12061 aggaagttgt agctggcgct cagctccagg taagggctcc agctgtcgtt cgggttatag
    12121 ccacgcagac cgctgcgagc ggactctttg cgcgatacgc cataatagta ttcgttctgg
    12181 ttttcgctgt tccactgcac accaataccc ggagtcacgg tcaggccacc gttggtgtaa
    12241 cgatacaacc aggccatatc ccagacaatg ccgttgctgt tatccagggt atcgccagcc
    12301 agggtggtac gcaggtaacc gtactgggta aagtgagcat aagacagacc agccatcatg
    12361 gtgctcttac ggtcatccag gtgacgcatt tggtgatcgc cactgtcttt cgctttgaag
    12421 taaagcggcg accagtaagc ggtaattgaa agtttatccg ttgcgtcatt ccacaggtag
    12481 taaccaccac ctaagccacg gaaccagaag ttatcgcctt catagttgat taccggtact
    12541 gggtaaacat cggtatcgta atctttatat gggtgctcaa cgacacctac gcctgcgccc
    12601 agggaaaatt taccttcagc gtgcgctacg cctgcagacg ttgcgataag cactccaagt
    12661 gccagaagtt tgagtttggt cacaattaat cattccttaa acaaatgttt agcgggcgac
    12721 aaagtttacc cgtcaataca tcgaaaccca acctttttac gttttcattt tttaaagtaa
    12781 ctgtttaatt ttcctgacgc ggatgacacc gcgcttacag ccaaatgaat ttagcgttac
    12841 tggctaccct ggtctttaca ttaattatgc aaaatttatg gatgagttgt tgatatgcca
    12901 ttgaaattaa gaaagccgtg caggcaagtt ttccatttgc catctacgct taattttgaa
    12961 ggtgtatcac cgggcacgtt gttctcatcg tcgataaaat ggcatgagag ttgctgtgtt
    13021 ttagcaagag acgtcgttca gtttacctct tccgggagcc tctactattc atatgaacgg
    13081 ctcttaacct gtgctaaaaa acgaaaggac ggcataccat gaatatattc gatcactatc
    13141 gccagcgata tgaagctgcc aaggacgaag agttcacact gcaggagttt cttaccactt
    13201 gtcggcaaga tcgcagtgct tatgccaacg cggctgagcg gctattgatg gctatcggtg
    13261 agcctgtcat ggtcgataca gcccaggaac ccagactttc tcgactcttt tctaaccggg
    13321 tcattgcacg ttatccggcg tttgaagagt tttacggcat ggaagacgcg attgaacaga
    13381 ttgtctctta tctgaaacac gcggctcagg ggctggaaga gaagaaacaa atcctgtatc
    13441 tgctggggcc tgtgggtggg ggtaaatcat cgcttgctga gcgactgaaa tcattaatgc
    13501 agctcgtacc gatttatgta ttgagcgcca acggtgagcg tagcccggtc aacgatcatc
    13561 cgttctgtct tttcaatccg caggaagatg ctcagattct ggaaaaagag tatggcattc
    13621 ctcgccgtta tctcggcacc atcatgtcgc catgggcggc aaaacgcctg catgaatttg
    13681 gtggcgatat cactaagttc cgggtagtga aggtctggcc gtcaattctg caacaaattg
    13741 ctatcgccaa aacggaaccc ggtgatgaga acaaccagga catctccgcg ctggttggga
    13801 aagtcgatat tcgtaaactc gaacactacg cgcagaatga cccggacgcc tacggctatt
    13861 ccggtgcgct gtgccgcgcc aaccaaggga tcatggagtt cgttgagatg tttaaagcac
    13921 cgataaaagt gctgcatccg ctgttaaccg ccactcagga aggtaactac aacgggacgg
    13981 aaggtatctc cgccctgccg ttcaacggga ttatcctcgc ccactcgaac gaatccgaat
    14041 gggtcacttt ccgtaataac aaaaacaacg aagcctttct cgaccgtgtt tacatcgtga
    14101 aggtgccgta ttgcttgcgc atttccgaag agatcaaaat ctacgagaaa ttgcttaatc
    14161 acagtgaatt gactcacgcc ccatgcgccc ctggcacgct cgaaacactg tcacgttttt
    14221 ccattctttc gcgcctgaaa gagccagaaa actccagcat ttattcaaag atgcgggttt
    14281 atgatggcga aagtctgaaa gacaccgatc ccaaagccaa gtcgtatcag gaatatcgtg
    14341 actacgccgg tgtcgatgaa gggatgaacg gtctgtcgac gcgttttgcg tttaagatcc
    14401 tctcccgcgt gttcaacttc gatcatgtag aagtggcagc aaacccggtc catctgttct
    14461 acgtcctgga acagcagatc gagcgcgagc agttcccaca agagcaggca gaacgctatc
    14521 tggagttcct gaaaggttat ctgatcccga aatatgccga gtttatcggc aaagagatcc
    14581 agacggccta ccttgaatcc tattccgaat atgggcaaaa cattttcgac cgttatgtta
    14641 cctacgcgga tttctggatt caggatcagg agtatcgcga tccggatacc gggcagctgt
    14701 ttgaccgcga gtctcttaac gccgagctgg agaaaatcga gaaaccggcg gggatcagta
    14761 atccaaaaga tttccgcaac gagattgtta acttcgtact gcgcgccaga gcgaataaca
    14821 gcggacgaaa tccgaactgg agcagctatg aaaaactgcg cacggttatc gagaagaaaa
    14881 tgttctccaa taccgaggag ctgttgccgg ttatctcgtt taacgccaaa acgtcaaccg
    14941 acgagcagaa gaaacacgac gactttgtcg accgtatgat ggaaaaaggc tacacccgta
    15001 aacaggtgcg tttactgtgc gaatggtatt tgcgcgtacg taaatcgtct taacaaccct
    15061 ggcccggtac acatgctacc gggcctacaa cgacagcgaa ccgtgggcca gagaaatggc
    15121 aacacaggcg tagcatacag ttggcaaatg tagtacgggg ggcatatgac ctggtttatt
    15181 gaccggcgtc tgaacggcaa aaacaaaagc atggtgaatc gccagcgttt tttacgccgt
    15241 tataaagcgc aaattaaaca gtcgatctcc gaggccatta ataagcgttc ggtgactgac
    15301 gtcgacagcg gcgaatccgt atccattccc acggaagata ttagcgaacc gatgtttcat
    15361 caggggcgtg gcggtctgcg ccaccgcgtg catccgggca atgaccattt cgtccagaac
    15421 gaccgaattg aacgtcccca gggtggcggc ggaggttccg gcagtggaca gggccaggcc
    15481 agccaggatg gtgaaggtca ggatgaattt gtctttcaga tttcgaaaga tgagtatctt
    15541 gatctgctct ttgaagattt ggctttaccg aatctgaaac aaaaccaaca acgccagctg
    15601 accgaatata aaacgcatcg ggcgggttat accgctaacg gcgttccggc caatatcagc
    15661 gttgtacgtt ccttgcagaa ctcactggcg cgtcgcacag ctatgacggc gggcaagcga
    15721 cgggaacttc atgcgctgga agagaatttg gccatcatca gcaacagtga acctgcgcaa
    15781 ctgctggaag aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa aattgaacgc
    15841 gtccctttca ttgacacctt cgatttacgt tacaagaact acgagaagcg gcccgatccc
    15901 tccagccagg cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga tcaatccact
    15961 aaagatatgg ctaagcgttt ttatattctg ctgtatctgt tcctcagcag aacgtataag
    16021 aacgtggaag tcgtatacat ccgccatcat acccaggcga aagaagtcga tgaacatgag
    16081 tttttctact cgcaggaaac aggcggcacc attgtttcca gcgccctgaa actgatggat
    16141 gaggtagtga aagagcgtta taacccggca cagtggaata tttacgctgc acaagcatcg
    16201 gacggcgata actgggccga tgactctccg ctttgccatg aaatcctggc gaaaaaatta
    16261 ttacctgttg ttcgttatta cagctatatc gaaattaccc gtcgtgcaca tcagacattg
    16321 tggcgagaat atgagcatct gcaatctact ttcgacaact ttgcgatgca gcacatccgc
    16381 gaccaggatg atatttatcc ggtattccgt gaactgtttc ataaacaaaa tgcaacagct
    16441 aaagactaaa actatcagcc gggtcattat cgcctggctg atttttagct tactgtaaat
    16501 tatctcattt attacataca ttagcttaca atcgctttaa atatgacagc ataaccttta
    16561 cataatttag ttccagaaaa caatcattcg gaaaaatgat tcagtcaaca cgtatttcca
    16621 tggggttatt ctttaaatat tttttatcgt taacgaaaat tgatcctggt caaaactata
    16681 tatctctgcc atcaataaaa tccagcactc acattgctct cctttttatg gtttctatgg
    16741 gtacacaaaa attaaaagct caaagctttt ttattttcag tttattgctg acgt
//
LOCUS       DAZFWP020000108        16740 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFWP020000108 DAZFWP020000000
VERSION     DAZFWP020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.108"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHK0Z_RS17055"
                     /old_locus_tag="pgaptmp_003411"
     CDS             complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHK0Z_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU4503376.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHK0Z_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHK0Z_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU4503377.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRAHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3791..5146
                     /gene="melA"
                     /locus_tag="ACHK0Z_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             3791..5146
                     /gene="melA"
                     /locus_tag="ACHK0Z_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU4503378.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5249..6670
                     /gene="melB"
                     /locus_tag="ACHK0Z_RS17070"
                     /old_locus_tag="pgaptmp_003414"
     CDS             5249..6670
                     /gene="melB"
                     /locus_tag="ACHK0Z_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU4503379.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHK0Z_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHK0Z_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU4503380.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHK0Z_RS17080"
                     /old_locus_tag="pgaptmp_003416"
     CDS             complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHK0Z_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4503381.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHK0Z_RS17085"
                     /old_locus_tag="pgaptmp_003417"
     CDS             complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHK0Z_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU4503382.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHK0Z_RS17090"
                     /old_locus_tag="pgaptmp_003418"
     CDS             complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHK0Z_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU4503383.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHK0Z_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHK0Z_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU4503384.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHK0Z_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHK0Z_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU4503385.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHK0Z_RS17105"
                     /old_locus_tag="pgaptmp_003421"
     CDS             13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHK0Z_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4503386.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14465..14761
                     /gene="ghoS"
                     /locus_tag="ACHK0Z_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             14465..14761
                     /gene="ghoS"
                     /locus_tag="ACHK0Z_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4503387.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14789..14962
                     /gene="ghoT"
                     /locus_tag="ACHK0Z_RS17115"
                     /old_locus_tag="pgaptmp_003423"
     CDS             14789..14962
                     /gene="ghoT"
                     /locus_tag="ACHK0Z_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU4503388.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15081..16598)
                     /gene="lysS"
                     /locus_tag="ACHK0Z_RS17120"
                     /old_locus_tag="pgaptmp_003424"
     CDS             complement(15081..16598)
                     /gene="lysS"
                     /locus_tag="ACHK0Z_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4503389.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         4083 a         3995 c         4259 g         4403 t
ORIGIN      
        1 cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt
       61 tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
      121 agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag
      181 tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta
      241 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
      301 ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg
      361 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgacttccg
      421 ccaccggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
      481 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
      541 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
      601 aggagcaaag ggtgttaacc agagttcccc atttcccacg ggttacgccc atagagaaca
      661 ggaacataat ttggaagtca gtggtgcggg taggtacaat gccgtggcga ccaagccagg
      721 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
      781 gagcaaggat gctgacttta atcgggtcga gcatactcca gttatccgga atatctttga
      841 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
      901 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
      961 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca
     1021 tcgcctgacg gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac aggccgctgt
     1081 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
     1141 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
     1201 cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg
     1261 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
     1321 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
     1381 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
     1441 agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt
     1501 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
     1561 tgccgtagcg gttgcggctt ggcaccatat agaccggttt cgcgcctgtc agcatcaaac
     1621 cttgttcgat ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc
     1681 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
     1741 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
     1801 gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat
     1861 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
     1921 ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg
     1981 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
     2041 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcagg tcgcgatcca
     2101 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
     2161 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
     2221 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
     2281 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
     2341 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
     2401 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
     2461 gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttatttttat aaacaatgcg
     2521 ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac tatttttttg
     2581 gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc
     2641 ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg
     2701 aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg
     2761 cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc
     2821 gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga
     2881 tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt
     2941 gcgcatggcg cgagacgctg tttttgtgtg cgcgcgaggt tttattgacc agaatcggtt
     3001 cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc
     3061 gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa
     3121 gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt
     3181 tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac
     3241 aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct
     3301 gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat
     3361 tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa
     3421 tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc
     3481 tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta
     3541 tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag
     3601 aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt
     3661 accataaact cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac
     3721 ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca
     3781 ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt
     3841 cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc
     3901 cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat
     3961 ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca
     4021 ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac
     4081 tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc
     4141 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat
     4201 gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac
     4261 ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca
     4321 gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc tgcgttaccg
     4381 ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg
     4441 cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa
     4501 gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa
     4561 gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat
     4621 taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa
     4681 acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc
     4741 ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac
     4801 tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc
     4861 acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa
     4921 agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac
     4981 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat
     5041 ggacccgcat actgctgccg tgctgggcat tgatgaaata tatgctcttg ttgacgacct
     5101 gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg
     5161 ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg
     5221 ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta
     5281 tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta
     5341 ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc
     5401 gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc
     5461 gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt
     5521 atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt
     5581 gacctacatc ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt
     5641 tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt
     5701 tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg
     5761 cggtggcgat cggggatttg gctttcagat gttcactctg gtactaatcg ccttttttat
     5821 tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc
     5881 gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga
     5941 tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac
     6001 cggatttgct atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta
     6061 tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa
     6121 atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg
     6181 tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg
     6241 tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga
     6301 taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt
     6361 gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt
     6421 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
     6481 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
     6541 tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata
     6601 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
     6661 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
     6721 acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc
     6781 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
     6841 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
     6901 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
     6961 ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg
     7021 ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga
     7081 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat
     7141 acggattacg gctcaacccc agccattcct gcacctcaaa accgatgggg actttcgcgt
     7201 aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca
     7261 gcggcgttaa cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg
     7321 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
     7381 ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     7441 tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     7501 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     7561 ttacttagtg cagttcgcgc actgtttgtt gacgatctgc tggaagaagt cgttaccttt
     7621 gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     7681 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     7741 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac
     7801 ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag
     7861 atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc
     7921 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     7981 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     8041 aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat
     8101 ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag
     8161 ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg
     8221 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca
     8281 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     8341 aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac
     8401 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc
     8461 gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc
     8521 gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt
     8581 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc
     8641 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     8701 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     8761 attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     8821 ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt
     8881 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa
     8941 ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     9001 ctgtttctgg tgtgccggac ggagcatgaa agaagcgtcg tgaaaggctt gctgcgccag
     9061 tagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     9121 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     9181 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     9241 gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac
     9301 atcgcggcga agatccagcc gaagacgcac gatacgctaa caccaatcaa ccccggcaga
     9361 ataaaactgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     9421 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     9481 gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc
     9541 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcatacgcc
     9601 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     9661 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     9721 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     9781 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     9841 aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg
     9901 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta
     9961 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
    10021 ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac
    10081 gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg
    10141 acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
    10201 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
    10261 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
    10321 atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
    10381 agcagcttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
    10441 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga
    10501 aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
    10561 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
    10621 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
    10681 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
    10741 ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact
    10801 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg
    10861 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt
    10921 tctcagggca gatatcatta ggtttaaaga ttaatctgat ctacccattt gtgggtaaaa
    10981 atacacataa tgcgggtgac ataatagtta attaactttt gttatcgttt tgaaattaaa
    11041 aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata attatatttg
    11101 atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg
    11161 gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt
    11221 gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg
    11281 atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca
    11341 cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg
    11401 tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga
    11461 ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa
    11521 tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag
    11581 cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta
    11641 atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta
    11701 tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc
    11761 aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct
    11821 gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc
    11881 attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg
    11941 gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc
    12001 cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg
    12061 tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat
    12121 cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac
    12181 cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca
    12241 gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca
    12301 gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg
    12361 gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga
    12421 gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca
    12481 atttattcat aaattcgtgg gatcgttcac gaagtgcgtc agcatagttg accagaccat
    12541 cgagtcgctg catcagttta cgtacttcag ttttgtccct gaaggttgaa atggcaccga
    12601 tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccga tctttaatcg
    12661 taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct
    12721 gtgaccatga gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca
    12781 gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga
    12841 cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt
    12901 agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca
    12961 gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt
    13021 gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt
    13081 catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga
    13141 cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac
    13201 gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca
    13261 gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt
    13321 tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct
    13381 tgttggctag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca
    13441 ccaatagcac cgagaacagt accgcactga ccattaagat cactgtggta ctcaatttca
    13501 tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac
    13561 aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg
    13621 ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg
    13681 ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact
    13741 ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat
    13801 atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa
    13861 gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg
    13921 cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa
    13981 ggccacaata aattttacat taatgacgaa caaggcaagc aaatcgctga aattgtcttt
    14041 gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa
    14101 gggcaaggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa
    14161 cgaaaaatta tcccattatg tccatttgcg aaacacgaat ttgataaaac gcgggagtat
    14221 gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcttg ctctccgcgg
    14281 agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagcgcg
    14341 tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga
    14401 atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg ataggagtca
    14461 tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt atccatcatc
    14521 ttattcctca gtgttcttaa ggttaagatc attgatgtat gatatgaatt tctcctctat
    14581 cgtggctgat gaatatggga taccacgaca attgaatgaa aactccttcg caataacgac
    14641 atcgttagcc gcaagtgaaa tcgaagattt aatcaggctt aaatgcttag acttaccgga
    14701 tattgatttt gacctcaaca ttatgacagt tgatgactat ttccgtcagt tttacaagta
    14761 gccaggaagg ataagaagaa cataaaaaat ggcactattc tctaaaatat taatttttta
    14821 tgtgattggt gtgaacatat cctttgtcat tatctggttt atctcacatg agaaaacaca
    14881 tattcgttta cttagtgcat tcctggtcgg aataacctgg ccaatgagtc tgcctgtggc
    14941 attacttttt tctctctttt aggagctgta gttgagtgga cccgctggtt atcgtgaaac
    15001 gaagcgtctc tcctcaaaac tggatttgcg gaacgacgaa cgaagcagtc gcctgactgc
    15061 ttcgttcatt aaagtgaaat ttatttctgt gggcgcatcg ccgggaagag aataacgtcg
    15121 cgaatagtat gactgtttgt aaacagcata atcattcggt cgataccaat acccagacca
    15181 gcggttggcg gcagaccata ttccagcgca gtcacgtaat cttcgtcata gaacatggct
    15241 tcgtcgtcac ctgcagcttt agcgttaacc tgttcctgga aacgttcagc ctgatcttct
    15301 gcgtcgttta attcgctaaa accattaccg atttcacgac caccgatgaa gaattcaaaa
    15361 cggtcggtga tttccgggtt aacatcatta cggcgcgcca gcggtgacac ttctgccgga
    15421 tattccgtaa taaaggttgg ctgaatcaga tgtgcttctg ccacttcatc aaagatctcc
    15481 gtgacaatac gtcccagccc ccagcttttc tctaccgtaa ttccgataga ttcagctaat
    15541 gctttagcag catcaaaatt atccaggtcg gccatatcgg tttctggacg atattttttg
    15601 attgcttcgc gcatggtgag tttttcaaac ggtttgccga aatcaaacac atgctcgcca
    15661 taagtgactt tagtggtacc cagaacctct tgtgccaggg tgcggaacag ggactctgtc
    15721 agttcaatca aatcgtggta atccgcatac gccatgtaga gttccatcat tgtgaactca
    15781 ggattatggc gaacagaaat accttcatta cggaagttac ggttgatttc gaatacccgt
    15841 tcaaaaccgc ctacaaccag acgtttcaga tacagctccg gcgcgatacg caggtacata
    15901 tctaaatcca gagcgttatg atgggtaata aacgggcgag cagatgcccc acctggaatt
    15961 acctgcatca tcggggtttc tacttccata aagccgcgcg cgaccatgaa ctggcggata
    16021 gcggccagaa tttttgaacg gacaacaaac gtttgacggg atttatcgtt agcgatgagg
    16081 tccagataac gttgacgata acggacttcc tgatcctgca gaccatggaa tttatctggt
    16141 aaaggacgta gtgcttttgt cagcaggcgt aactcagtac agtgaatgga aagctcaccg
    16201 gtttgcgtct taaacagcgt accgcgggca ccgataatgt cacccagatc ccatttttta
    16261 aactgatcgt tataaacacc ttctggcagg ctatctcttg caacgtacag ttgaatacgg
    16321 ccaccgacat cctgcaacgt tacaaaggag gctttcccca tgatacgacg ggtcatcatt
    16381 cggccagcaa ccgagacttc aatgtttaag gattccagtt cctggttatc cttcgcatca
    16441 aactcttcgt gcagctggtc agaggtatgg tcgcggcgaa aatcattggg aaacgccaca
    16501 ccttgctgac gtagtgccgc cagtttttcg cggcgatttc tcagttcatc gttaaaatca
    16561 atagcctcat tggctccccg tgtttcttgt tcagacattt tggttcctct aaatccagct
    16621 ttcaatttag cgtagataaa gagacagatc ggtacactgt attatctgcc tcgactatta
    16681 ataactcagt ttatcttatc gaaactaacg cttacctcaa gttgatatca ctaataaata
//
LOCUS       DAZFWP020000109        16046 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFWP020000109 DAZFWP020000000
VERSION     DAZFWP020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.109"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            17..340
                     /locus_tag="ACHK0Z_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             17..340
                     /locus_tag="ACHK0Z_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4503390.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGERKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(443..1693)
                     /gene="icd"
                     /locus_tag="ACHK0Z_RS17130"
                     /old_locus_tag="pgaptmp_003426"
     CDS             complement(443..1693)
                     /gene="icd"
                     /locus_tag="ACHK0Z_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU4503391.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1865..2518
                     /gene="rluE"
                     /locus_tag="ACHK0Z_RS17135"
                     /old_locus_tag="pgaptmp_003427"
     CDS             1865..2518
                     /gene="rluE"
                     /locus_tag="ACHK0Z_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU4503392.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2528..2989
                     /gene="nudJ"
                     /locus_tag="ACHK0Z_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             2528..2989
                     /gene="nudJ"
                     /locus_tag="ACHK0Z_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU4503393.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3043..4149
                     /gene="mnmA"
                     /locus_tag="ACHK0Z_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /gene_synonym="trmU"
     CDS             3043..4149
                     /gene="mnmA"
                     /locus_tag="ACHK0Z_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU4503394.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4185..4826
                     /gene="hflD"
                     /locus_tag="ACHK0Z_RS17150"
                     /old_locus_tag="pgaptmp_003430"
     CDS             4185..4826
                     /gene="hflD"
                     /locus_tag="ACHK0Z_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU4503395.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4830..6200
                     /gene="purB"
                     /locus_tag="ACHK0Z_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     CDS             4830..6200
                     /gene="purB"
                     /locus_tag="ACHK0Z_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU4503396.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6368..7039
                     /gene="phoP"
                     /locus_tag="ACHK0Z_RS17160"
                     /old_locus_tag="pgaptmp_003432"
     CDS             6368..7039
                     /gene="phoP"
                     /locus_tag="ACHK0Z_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU4503397.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7039..8499
                     /gene="phoQ"
                     /locus_tag="ACHK0Z_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             7039..8499
                     /gene="phoQ"
                     /locus_tag="ACHK0Z_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU4503398.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8575..9696
                     /gene="roxA"
                     /locus_tag="ACHK0Z_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /gene_synonym="ycfD"
     CDS             8575..9696
                     /gene="roxA"
                     /locus_tag="ACHK0Z_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU4503399.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9842..11071)
                     /gene="pepT"
                     /locus_tag="ACHK0Z_RS17175"
                     /old_locus_tag="pgaptmp_003435"
     CDS             complement(9842..11071)
                     /gene="pepT"
                     /locus_tag="ACHK0Z_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU4503400.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCNIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11321..12457
                     /gene="potA"
                     /locus_tag="ACHK0Z_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             11321..12457
                     /gene="potA"
                     /locus_tag="ACHK0Z_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU4503401.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12441..13304
                     /gene="potB"
                     /locus_tag="ACHK0Z_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             12441..13304
                     /gene="potB"
                     /locus_tag="ACHK0Z_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU4503402.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            13667..15028
                     /gene="espK"
                     /locus_tag="ACHK0Z_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             13667..15028
                     /gene="espK"
                     /locus_tag="ACHK0Z_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HGU4503403.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(15405..>16046)
                     /locus_tag="ACHK0Z_RS17195"
                     /old_locus_tag="pgaptmp_003439"
     CDS             complement(15405..>16046)
                     /locus_tag="ACHK0Z_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGU4503404.1"
                     /translation="QVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYD
                     EDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCRGTT
                     GIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFSREH
                     YLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT         4192 a         3952 c         3832 g         4070 t
ORIGIN      
        1 ggataagaca taacaaatga agtggatagt aattgacacg ataattcaac cttcatgcgg
       61 catatctttt tcagccatat ggggtaatat gaaaatgatc atctggtatc aatctactat
      121 atttctccct cctggcagta tatttacacc ggttaaatct ggaattatcc ttaaagataa
      181 agaatatcct attactattt ataacatcgc accattcaac aaggatttat ggagtctact
      241 caaaagcagt caagagtgtc ctccaggaga aagaaaaata acaaataaat gtttgcataa
      301 tagttgcatt ataaaaatat gcccatatgg gctcaagtaa agggttttat ataaatatgc
      361 tcgatatgct attaataata cctacataaa taaaacaacg ggcgtgttat acgcccgttg
      421 caatattaaa cacatgtagt gattacatgt tcttgatgat cgcatcacca aattctgaac
      481 atttcagcag tttagcgcct tccatcagac gctcgaagtc ataggttacg gtcttcgcat
      541 tgattgcgcc ttccatacct ttaacaatca ggtctgcggc ttcagtccag cccatatggc
      601 gtaacatcat ctcagcggag agaataatag agccagggtt tactttgtcc tgaccggcgt
      661 atttcggcgc agtaccgtgg gtggcttcaa acagggcgca ttcgtcaccg atgtttgcac
      721 caggggcgat accgataccg ccaacctgcg ctgccagggc gtcagaaatg tagtcaccgt
      781 tcaggttcat acaggcgata acatcatatt cagccggacg cagcaggatc tgttgcagga
      841 atgcatcagc aatcacgtct ttaatgacga tctctttgcc ggtgttcggg tttttaactt
      901 tcagccacgg gccgccgtcg atcagttcac cgccaaactc ttcacgcgcc agctggtagc
      961 cccagtcttt aaacgcgcct tcggtgaact tcatgatgtt gcctttgtgc accagggtca
     1021 cagagtcacg gtcgttggca attgcgtatt caatcgcggc acggaccaga cgtttggtgc
     1081 cttcttcaga acacggcttg ataccgatac cgcaatgttc cgggaagcga attttcttca
     1141 cgcccatctc ttcacgcagg aatttaatca ctttctcggc gtcggcagag tctgctttcc
     1201 attcgatacc cgcataaatg tcttccgagt tttcacggaa gataaccata tcggtcagtt
     1261 cagggtgttt aaccgggctt ggagtgcctt gatagtaacg taccggacgc aggcagatgt
     1321 agagatccag ttcctggcgc agggcaacgt tcagagagcg aataccgcca ccaacaggag
     1381 tggtcagcgg acctttaata gcaacgcgat attcacgaat cagatcaagg gtttcagcag
     1441 gcagccagac atcctgacca taaacctgtg tggatttttc accggtgtaa atttccatcc
     1501 aggagatttt acgctcgcct ttataggctt tctcgactgc agcgtcgacc actttcagca
     1561 tggctggggt tacatctaca ccgattccat caccttcaat gtaagggata atcggatttt
     1621 caggaacgtt gagtttgccg ttttgcaggg tgatcttctt gccttgtgcc ggaacaacta
     1681 ctttactttc cattcacctc tccttcgagc gctactggtt tgctcgtctg ccaccacgtt
     1741 gcatatgcgt ttgcgtcctg cgatacggat gctttagagc aattttttgt taatgatttg
     1801 taattggctt gtcaatacta ccctattgtt tgccgtcatg aaagatgcgc gttattaggc
     1861 tataatgcgg caattcataa tctctgaaaa taccatgcaa aaaacttctt ttagaaatca
     1921 ccaggttaag cgattcagct cgcaacgttc taccaggcgt aaacctgaaa accagcccac
     1981 gcgtgtgatc ctgttcaata aaccctacga tgttcttccg cagttcaccg atgaagccgg
     2041 acgcaaaaca ttaaaagaat tcatcccggt tcagggcgtt tatgcagcag gtcgtcttga
     2101 ccgcgatagc gaagggttac tggtgctgac caataacggc gcgttgcagg cgcgtttaac
     2161 ccagccgggt aaacgcaccg ggaaaatcta ttatgtgcag gtggaaggta ttcccacaca
     2221 agacgcactt gaagccttgc gcaatggcgt aaccttaaat gatggcccta ccctgcccgc
     2281 cggcgcggag ctggttgacg aaccagcgtg gttatggccg cggaatccac caattcgtga
     2341 acgcaaaagt attcccacca gctggctgaa gatcacctta tatgaaggac gtaatcgcca
     2401 ggtgcgccgc atgaccgccc atgttggttt ccccacgctg cgactgattc gctatgcgat
     2461 gggagattac tctttggata atcttgccaa tggcgaatgg cgagaagtga cagattaagg
     2521 atgcaaaatg tttaaaccgc acgttaccgt tgcttgcgtg gtgcacgcag aaggcaaatt
     2581 tttagtcgtt gaagagacga ttaatggtaa agcgttatgg aaccaacctg ccgggcatct
     2641 ggaagccgat gaaaccttag tggaagccgc cgcccgtgaa ctgtgggaag aaaccggcat
     2701 cagcgcgcag ccgcaacact ttattcgtat gcatcagtgg attgcgccag ataaaacgcc
     2761 gtttttgcgt ttcctctttg ccattgagct tgagcaaata tgcccgaccc agcctcatga
     2821 cagcgatatc gactgctgtc gttgggtcag cgccgaagag attttaaagg cgtcaaatct
     2881 tcgttcgccg ctggtggcgg aaagtatccg ttgttatcaa agcgggcaac gctatccgct
     2941 ggagatgatt ggcgatttta actggccttt tacaaagggt gtcatctaaa aggctgcgtg
     3001 atagaatacg ccgccttgaa gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa
     3061 aaagtaatcg tcggcatgtc cggcggtgtc gattcctccg tttctgcctg gctgttgcaa
     3121 caacagggat atcaggttga aggcctgttt atgaagaact gggaagaaga cgacggtgag
     3181 gaatattgca cagcggcagc ggatctggct gatgcccagg ctgtctgcga caagctcggc
     3241 attgaactgc acaccgttaa ctttgctgcc gagtactggg acaacgtctt cgaactgttc
     3301 cttgccgaat ataaagccgg tcgcacgccg aatccggata ttctgtgcaa caaagagatc
     3361 aaatttaaag ccttcctcga atttgccgcc gaagatttag gtgccgatta tatcgctacc
     3421 ggtcattacg tacgtcgggc cgatgtcgat ggcaagagcc gcctgctgcg tggtctggac
     3481 agcaataaag accagagcta cttcctttat acgctcagcc atgagcagat tgcgcaaagc
     3541 ctgttcccgg tcggcgaact ggaaaaaccg caggtgcgta agattgctga agatcttggt
     3601 ctggtcaccg cgaagaaaaa agactctacc ggcatttgct tcattggcga acgtaaattc
     3661 cgcgagttcc tgggccgtta tctcccggcg caaccgggca aaatcattac cgtcgatggc
     3721 gatgaaattg gtgagcacca ggggctgatg tatcacactc tcggtcagcg taaaggtctg
     3781 ggtatcggtg gcaccaaaga aggtaccgaa gaaccgtggt atgtggtgga caaagacgtc
     3841 gaaaacaaca ttctggttgt cgctcagggc catgaacacc cgcggctgat gtctgtcggg
     3901 ttgattgccc agcagttgca ctgggtcgat cgcgaaccat tcaccggcac tatgcgttgc
     3961 acggtaaaaa cccgctatcg ccagaccgat atcccttgca ccgtcaaggc gctggacgat
     4021 gatcgcattg aagtgatttt cgatgaaccg gttgccgccg tgacgccggg ccagtctgcc
     4081 gtcttctata acggtgaagt gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg
     4141 ccggtctgat tattatcttt acttaaaaca aggaagcagt gaacgtggca aagaattact
     4201 atgacatcac cctcgccctg gccggtattt gtcagtcggc acgcctagtg caacaactcg
     4261 ctcaccaggg gcattgtgat gccgatgcgc tacacgtctc actcaacagt attattgata
     4321 tgaaccccag ctcgacgctg gcggtttttg gcggtagcga agccaacctg cgcgtcgggc
     4381 tggaaaccct gctcggcgtg ctcaatgcca gcagtcgcca gggcttaaac gccgaattaa
     4441 cccgctacac actcagcttg atggtgcttg agcgcaaact ctcctcagcg aaaggcgcgc
     4501 tcgacactct gggcaaccgg atcaacggcc tgcaacgcca gctcgaacac ttcgatttac
     4561 agtccgaaac gctgatgagc gcgatggctg ctatctatgt tgatgtgatc agcccgcttg
     4621 gcccgcgcat tcaggtcacc ggttcccctg ctgtactgca aagcccacaa gtgcaggcga
     4681 aagttcgcgc aaccctgctg gcaggcattc gcgccgccgt gctctggcac caggtcggcg
     4741 gcggacgtct gcaactgatg ttttctcgta atcgcctgac cactcaggca aaacaaattc
     4801 ttgctcattt aaccccggag ttgtgatcta tggaattatc ctcactgacc gccgtttccc
     4861 ctgtcgatgg acgctacggc gataaagtca gcgcgctgcg cgggattttc agcgaatatg
     4921 gtttgctgaa attccgtgta caagttgaag tacgttggct gcaaaaactg gccgcgcacg
     4981 cagcgatcaa ggaagttcct gcttttgctg ccgacgcaat cggttacctt gatgcaattg
     5041 tcgccaattt cagcgaagaa gatgccgcac gcatcaaaac catcgagcgt accactaacc
     5101 acgacgttaa agcggttgag tatttcctga aagaaaaagt ggcggagatc ccggaactgc
     5161 acgcggtttc tgaattcatc cactttgcct gtacttcgga agatatcaat aacctctccc
     5221 acgcattaat gctgaaaacc gcgcgtgatg aagtgatcct gccgtactgg cgtcaactga
     5281 ttgatggcat taaagatctc gccgttcagt accgcgatat cccgctgctg tctcgtaccc
     5341 acggtcagcc agccacgccg tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta
     5401 tggagcgcca gtaccgccag cttaatcagg tggagatcct cggcaaaatc aacggcgcgg
     5461 tcggtaacta taacgcccat atcgccgctt acccggaagt tgactggcat cagttcagcg
     5521 aagagttcgt cacctcgctg ggtattcagt ggaacccgta caccacccag atcgaaccgc
     5581 acgactacat tgccgaattg tttgattgcg ttgcgcgctt caacaccatt ctgatcgact
     5641 ttgaccgtga cgtctggggt tatatcgccc ttaaccactt caaacagaaa accattgctg
     5701 gtgagattgg ttcttccacc atgccgcata aagttaaccc gattgacttc gaaaactccg
     5761 aagggaatct gggcctttcc aacgcggtat tgcagcatct ggcaagcaaa ctgccggttt
     5821 cccgctggca gcgtgacctg accgactcca ccgtgctgcg taacctcggc gtgggtatcg
     5881 gttatgcgct gattgcgtat caatccaccc tgaaaggcgt gagcaaactg gaagtaaacc
     5941 gtgaccatct gctggatgag ctggatcaca actgggaagt gctggctgag ccaatccaga
     6001 cagttatgcg tcgctatggc atcgaaaaac cgtacgagaa gctgaaagag ctgactcgcg
     6061 gtaagcgcgt tgacgccgaa ggcatgaagc agtttatcga tggtctggcg ttgccagaag
     6121 aagagaaagc ccgcctgaaa gcgatgacgc cggctaacta tattggtcga gctatcacca
     6181 tggttgatga gctgaaataa actcgtatca gtgccggatg gcgatgctgt ccggcctgtt
     6241 tattaaaatt atccactttt tcttttttca ctttacctcc cctccccgct ggtttattta
     6301 atgtttaccc ccataaacac ataatcgcgt tacactattt taataattaa gacagggaga
     6361 aataaaaatg cgcgtactgg ttgttgaaga caatgcgttg ttacgtcacc accttaaagt
     6421 tcagattcag gatgctggtc atcaggtcga tgacgcagaa gatgccaaag aagccgatta
     6481 ttatctcaat gaacatttac cggatattgc gattgtcgat ctcggattgc ctgacgagga
     6541 cggtctgtca ctgattcgcc gctggcgtag caatgatgtt tcactgccga ttctggtatt
     6601 aaccgcccgt gaaagctggc aggacaaagt cgaagtatta agtgccggtg ctgatgatta
     6661 cgtgactaaa ccgtttcata ttgaagaggt gatggcgcga atgcaggcat taatgcggcg
     6721 taatagcggc ctggcttcac aggtcatttc gctacccccg tttcaggttg atctctctcg
     6781 ccgtgaatta tctattaatg acgaagtgat caaactgacc gcgttcgaat acaccattat
     6841 ggaaacgttg atacgcaata atggcaaagt ggtgagcaaa gattcgttaa tgctccaact
     6901 ctatccggat gcggagctgc gggaaagcca taccattgat gtactgatgg gacgtctgcg
     6961 caaaaaaatt caggcacaat atccgcaaga agtgattact accgttcgcg gccagggcta
     7021 tttgttcgaa ttgcgctgat gaaaaaatta ctgcgtcttt ttttcccgct ctcactgcgg
     7081 gtacgttttc tgttggcaac ggctgcggta gtactggtgc tttcgcttgc ctacggaatg
     7141 gtcgcgctga tcggttatag cgtcagtttc gataaaacta cgtttcggct gttacgtggc
     7201 gagagcaatc tgttctatac ccttgcgaag tgggaaaaca ataagttgca tgtcgagtta
     7261 cccgaaaata tcgacaagca aagccccacc atgacgctaa tttatgatga gaacgggcag
     7321 cttttatggg cgcaacgtga cgtgccctgg ctgatgaaga tgatccagcc tgactggctg
     7381 aaatcgaatg gttttcatga aattgaagcg gatgttaacg ataccagcct cttgctaagt
     7441 ggagatcatt cgatacagca acagttgcag gaagtgcggg aagatgatga cgacgcggag
     7501 atgacccact cggtggcagt aaacgtctac ccggcaacat cgcggatgcc aaaattaacc
     7561 tttgtggtgg tggataccat tccggtggag ctaaaaagtt cctatatggt ctggagctgg
     7621 tttatctatg tgctctcagc caatctgctg ttagtgatcc cgctgctgtg ggtcgccgcc
     7681 tggtggagtt tacgccccat cgaagccctg gcaaaagaag tccgcgaact agaagaacat
     7741 aaccgcgaat tgctcaatcc agccacaacg cgagaactga ccagtctggt acgaaacctg
     7801 aaccgattgt taaaaagtga acgcgaacgt tacgacaaat accgtacgac gctcaccgac
     7861 ctgacccata gtctgaaaac gccactggcg gtgctgcaaa gtacgctgcg ttctctgcgt
     7921 agtgaaaaga tgagcgtcag tgatgctgag ccggtaatgc tggagcaaat cagccgcatt
     7981 tcacagcaaa ttggctacta cctgcatcgt gccagtatgc gcggcgggac attgctcagc
     8041 cgcgagctgc atccggtcgc cccactgctg gacaatctca cctcagcgct gaacaaagtg
     8101 tatcaacgca aaggggtcaa tatctctctc gatatttcgc cagagatcag ctttgtcggt
     8161 gagcagaacg attttgtcga ggtgatgggc aacgtgctgg ataatgcctg taaatattgc
     8221 ctcgagtttg tcgaaatttc tgcaaggcaa accgacgagc atctctatat tgtggtcgag
     8281 gatgatggcc ccggtattcc attaagcaag cgagaggtca ttttcgaccg tggtcaacgg
     8341 gttgatactt tacgccctgg gcaaggtgta gggctggcgg tagcccgcga aatcaccgag
     8401 caatatgagg gtaaaatcgt cgccggagag agcatgctgg gcggtgcgcg gatggaggtg
     8461 atttttggtc gccagcattc tgcgccgaaa gatgaataaa tatgtccata cttcacgcat
     8521 tacgttaagc atccgttata atcggttgca gataccagcc tgtggatgct taacatggaa
     8581 taccaactca ctcttaactg gcccgatttt cttgaacgtc actggcagaa acgcccggtg
     8641 gtgttaaaac gcggctttaa taattttatt gacccgatct ctccagacga gttggcgggt
     8701 ctggcgatgg aaagcgaagt tgacagtcga ctggtcagtc accaggatgg caaatggcag
     8761 gtcagccacg gcccgttcga aagctacgat catctcggtg aaaccaactg gtcattactg
     8821 gtacaggcag tgaaccactg gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa
     8881 ctaccggact ggcgtattga tgatctgatg atctcttttt ctgtaccggg cggcggcgtc
     8941 ggcccgcatc tcgatcagta cgacgtgttt atcattcagg gtaccggtcg tcgtcgctgg
     9001 cgagtgggcg aaaagctgca aatgaaacag cactgtccgc acccggatct gttacaggtc
     9061 gatccgttcg aagccatcat cgatgaagag ctggagcctg gcgatattct ttatattccg
     9121 ccaggattcc cgcatgaagg ctacgcgctg gaaaatgcga tgaactattc cgtgggtttt
     9181 cgcgcgccaa atacgcggga attaattagc ggatttgccg attatgtgct gcaacgtgaa
     9241 ctgggcggca actactacag cgatccggat gttccacctc gcgctcatcc tgcggacgtt
     9301 ctgccgcaag agatggataa actgcgtgag atgatgctcg aattgatcaa ccagccggaa
     9361 cactttaagc aatggtttgg cgagtttata tcccagtcac gtcatgaact ggatatcgcg
     9421 ccgccggaac cgccttatca gccagatgaa atctacgatg cgctgaaaca aggtgaagtg
     9481 ctggtgcgcc tgggtggtct gcgcgtattg cgcattggcg acgacgtgta tgccaatggt
     9541 gagaagatcg attccccgca ccgtccggca ctggatgcac tcgccagcaa cattgcgctg
     9601 actgcggaga attttggcga tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg
     9661 ctggtcaata gcgggtattg gttcttcgaa gggtaagttt ggatttaagc cggatgcggc
     9721 gctgattgcg ccttatcgtg cctacatgca ctgaacgcct gtaggcacga taagtatgtt
     9781 gaccagtatc aatataacgg tttcatcccg gtaattggca gtccacatac gcatcgggca
     9841 atcacgactt ccgttgcgcc gttaactcgg caatacggac aatcacctgc accgcttttt
     9901 ccataccttc cagagtcaca aactcatgct taccatgata gttgtaaccg ccagtgaaca
     9961 ggttcgggca cggtaatccc ataaacgaca actgcgcgcc gtcggtaccg ccgcggatcg
    10021 gtttcagttc cggttcaata ttgcagtcac gcatcgcctg ctgggcgata tcgataatat
    10081 gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc accagttcaa
    10141 tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc attttacgtt
    10201 tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata tcggctcggt
    10261 caacggtgcc tttcatgctc gccaggtgat agaaaccttc atagccttct gtcatttccg
    10321 ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc gcatttacca
    10381 tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata ttgaccgatg
    10441 cggcgttgaa gttttcaaac tccagttcac ctacgccacc accgtcaaca gtgtaagccc
    10501 agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact tcttcatccg
    10561 gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat accgccagcg
    10621 cggtcatgat ttctgcaata ccggctttgt catcggcacc taacaaggtt ttaccatcgg
    10681 tagtaatcag cgtctgaccc agtagctgat gcagcaccgg gaacataacc ggtgataaaa
    10741 cttcatcgcc gatacccagc gcaatatcgc caccgcgata gttttcaaca atttgcggat
    10801 tcacattttt gccgctgcaa tccggtgagg tatccacatg agaaataaag ccaatcgccg
    10861 ggatatcgcc agggacgtta gccggtaacg tcgccatcaa agtgcccttc tcacttaagg
    10921 tcacattgat aagccccatc tcttcgagct gctctttcag cagatgcaat aacttccatt
    10981 ggccttccgt gctgggaacc tgtctcaccc ctgcttttga ttgggtatcc agagacacgt
    11041 agttcaaaaa tcgctcaagt agtttatcca tgtagtcacc ctcacttttt gtgacaacat
    11101 tattaagaag caagaaaaga caaatattgc gtcaggtcac ttttacccct gcaagcggga
    11161 atatttatca gcattacctt aatgaatata aagctaagcc agtaattcac aacaaagatt
    11221 ggcgattcaa gcggtttgcc gtagaattac cgccctcatt aagagtcacc aaggtggtta
    11281 accacaaacc ccgcatcggt aagccatccg ttgcgtttac atgggacaga gtaaaaaatt
    11341 gaataaacaa ccgagttcgc tttcaccgct ggtgcaattg gcgggaattc gcaaatgctt
    11401 tgatggtaaa gaggtcattc cccagctgga tctgactatc aacaatggcg agttcctcac
    11461 gctgcttggc ccttctggct gcggtaaaac aaccgttctt cgcctgattg caggtctgga
    11521 aactgttgat tccggacgca tcatgctgga taacgaggac atcacccacg ttccggcgga
    11581 aaaccgctat gtgaacactg ttttccaaag ctacgcactt ttcccccaca tgaccgtgtt
    11641 cgaaaatgtg gcctttgggt tgcgcatgca aaaaaccccc gctgctgaaa ttacgccccg
    11701 cgtgatggaa gccctgcgga tggtgcaact ggaaaccttc gctcaacgta aaccgcatca
    11761 gctctctggt ggtcaacagc aacgcgtcgc cattgcccgc gcggtggtta acaagcctcg
    11821 tctgttgttg ctggatgagt cgctctcagc gctggattac aaactgcgca agcaaatgca
    11881 gaacgagctg aaagcgttac agcgtaagct tggcattaca ttcgtcttcg tgactcacga
    11941 ccaggaagaa gcactcacca tgtcagacag gattgtggtg atgcgcgatg gtcgcattga
    12001 gcaagacggc acgccgcgtg aaatctacga agagccgaaa aacctgtttg ttgccgggtt
    12061 cattggcgaa attaatatgt ttaacgccac ggtgatcgag cgactcgacg agcagcgcgt
    12121 acgcgccaac gttgaaggcc gcgaatgtaa tatctacgtt aacttcgccg ttgaaccggg
    12181 gcaaaaactg catgttctgc tgcgcccgga agacttacgt gttgaagaga ttaacgacga
    12241 caaccacgct gaagggctga ttggttacgt tcgtgagcgt aactacaaag gcatgacgct
    12301 ggagtcggtt gttgaactgg aaaatggcaa gatggtgatg gtcagcgaat tcttcaatga
    12361 agacgaccct gactttgacc actctctcga ccaaaaaatg gccattaatt gggtagaaag
    12421 ctgggaggtc gtactggctg atgaagaaca caagtaagtt ccagaatgta gtgattgtca
    12481 ctattgtcgg ttggcttgtg ttgtttgtct ttctgcccaa cctgatgatc attggcacca
    12541 gctttttgac ccgcgacgac gccagtttcg tcaaaatggt ctttacgctg gataactaca
    12601 cgcgtctgct cgatccgctc tattttgaag tgctattgca ctcgctgaat atggcgctga
    12661 tcgccaccct cgcctgcctg gtgctgggct acccgtttgc ctggtttctg gcgaagctgc
    12721 cacacaaggt gcgtccgctg ctgctgtttc tgctgattgt tcccttctgg accaactcat
    12781 tgattcgtat ctacgggctg aaaattttcc tcagcaccaa aggctatctc aacgagtttt
    12841 tgctctggct gggcgttatc gacacaccaa tccgtatcat gttcacgccc agtgcggtga
    12901 ttatcggtct ggtttacatt ctgctgccgt ttatggtgat gccgctgtac tccagtatcg
    12961 aaaaactgga taagcctttg cttgaggcgg cgcgcgatct cggtgccagc aagttgcaga
    13021 cctttatccg catcattatc ccactgacga tgccggggat tattgccgga tgtctgctgg
    13081 tgatgctgcc agcgatgggc ctgttctatg tgtccgacct gatgggcggt gcgaaaaacc
    13141 tgctgatcgg taacgtcatc aaggtacagt tcctcaatat tcgtgactgg ccatttggtg
    13201 cagccaccag cattacgctg actatcgtaa tgggcctgat gttgctggtt tactggcgcg
    13261 cttctcgtct gctgaataag aaggcaacag aaatagataa ttagtgcttt atattcaaaa
    13321 ggttaatagc aaatttggca tgctttttac tgtctagtaa acagcatgcc tctatccata
    13381 aaatcaaaca gttgcaatct aattttggag aacgtttttt cttctccctt ttagcaaatc
    13441 gctataaaaa acaacaaatg gtataaaaat gagcaatcct aatccatgca tgacggactg
    13501 gagaaagtat tcgcaaaagt taaaatcaac agtctgttga ttttgagata taactctctt
    13561 ttctaatatt gacaccttgg ctttgctggt tgctaaaatt agcttgttga ttagcagtat
    13621 ggatagaact tacactgtta tttacttcta aaaatggaat ttatcaatgc ttcctacatc
    13681 gcaattacga ccgtccggga cattctactc caattccgca gaaacatcag caggcatcaa
    13741 aagcgaaatc acaccaactc agatagaaga agcgcgggcc attggtcgtt tatatatcaa
    13801 agattgtgat attgagtatc tgccacagtt accaaacgaa ataacatcag ttacaatcga
    13861 aaattgcaat aacctgacca cccttacagg attaccggtt aatacacaaa acctgtccgt
    13921 cattaactgt gaaaaattac aaatcacaga cataccatcg acagtaaaaa atctacatat
    13981 tgaattaact gattcaccat ttatacacat catatctgaa ggcatcgaat gcctgacggt
    14041 ttgccactgc catatatctg gagtgccaga gagtgtccgt catcttgaga tcagaggtta
    14101 cgccacagac ggcataaaaa atgttccaaa cgggttatta tctctcagca tcaatcgcta
    14161 caccccagaa aatcaggcca gaattgacag cctgatatca ccgtcactac agacgctatc
    14221 tctgactgga tgtagcaata ttatactgcc ggagaaactc ccggagagtc tgagatctgt
    14281 aaccattcat gtggagcaga aaactacatg gaacatcgta gctgaagggg ttcctgatgg
    14341 actggatctt gatttacaaa atgtactact ctctccgaat gtaattaaag aaaaaaacat
    14401 cacttttcag ggcaacgcac tggatgctgc cttgcatttt cgtccgggag acattgtcta
    14461 tgggctatcc tctcccagag aaaaacttgt caacagcatc aaactggtta acgacttttc
    14521 caaaaaagat atcgtaattc agaatacatt aacaaacgca gtatgggacg ccagaacccc
    14581 ccgtaaatat aagcaagact cgcttatcaa aagagcatta aatgaacatg aaagaggaac
    14641 aaaatttaaa cagtgcttaa aaaatcacaa taattataat gtcatcatgg ccgacctttc
    14701 catatacaat cgagacaagt tatgggcaaa aacaagcaag gcaggcctag agtttcaaac
    14761 attaattcgc aacagaacgg ttatgttttg tgcggatggg cttgtcaatt cactcaaact
    14821 catagctaac aagtcagagg gctatggtca gagtattacc gccagcgaat tacgatggat
    14881 ttatcgtaat aaagacaaca gccaaataat gaaaaacata aaattttatc tgcatggcaa
    14941 agagatacca gcagaaagaa tattagatac accagagtgg aaagactacc gtccaaaata
    15001 ctccggttcc acatataaat attcttaatg atagcaaaaa atatattttc gatataatca
    15061 atgttatgat taaagagtat ttcatcaggg caggtaaaaa cagagtaaat cagcaaaaga
    15121 agctgatctt cagcgatact gacactaact gacggtttaa gcggtcgtat gaagcagcag
    15181 ctttccgacg gactgccatg cggatcgttt accttttggg ctattccgcc cgtcatcaag
    15241 cggctcacga gtactgagtt tatcaggatg atattgctga caatggtaat tcgttgaccg
    15301 atgtgtactt cactatatat cggtcaacac cgtactgccg tgcaacatct caaagatgta
    15361 gcatcgtttt taccagagga ggcaggacat agattgtaaa aaaactattt cgatgcattt
    15421 accattgaga tattttcctt cataccaaaa gttctatcaa ttatcgcaag tggttcaaca
    15481 ggtgcggtct tatctataac ccaaccactc tctgtaacac ttcctcgtgt gatatatttt
    15541 gcctttactt taacaactat tagataatgc tcacgactaa accgatcagc aacgctaaga
    15601 tccgatgtgt attcaggcaa tattcttcca atacgtacct gctgttgtgc catatattca
    15661 tctggtgctt ttacatttgg atttgaatca ggaagccctc cagcagtttt atattcaacg
    15721 atattttcag cttgaatacc ggttgtgccg cgacacaata tgatgtctga atcggacagt
    15781 tctatgtgcc cctccttaat tttcgaataa atttgtttaa tactatgttc tggagttggt
    15841 actatttcag aagataaagc aggcaacgtg tcttcaagca ccggagaatc atcgtatata
    15901 tccaacaaat cctcatcata cccaatatct ccttctctta actgagaata cggtttatct
    15961 atgtatctaa taactccccc accatgaaga ccttctgatt tataaaatgt accatcagcg
    16021 tcacgaaaga aataaacatt aacctg
//
LOCUS       DAZFWP020000110        15885 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFWP020000110 DAZFWP020000000
VERSION     DAZFWP020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.110"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(21..746)
                     /gene="phoU"
                     /locus_tag="ACHK0Z_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             complement(21..746)
                     /gene="phoU"
                     /locus_tag="ACHK0Z_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU4503405.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(761..1534)
                     /gene="pstB"
                     /locus_tag="ACHK0Z_RS17205"
                     /old_locus_tag="pgaptmp_003441"
     CDS             complement(761..1534)
                     /gene="pstB"
                     /locus_tag="ACHK0Z_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU4503406.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1625..2515)
                     /gene="pstA"
                     /locus_tag="ACHK0Z_RS17210"
                     /old_locus_tag="pgaptmp_003442"
     CDS             complement(1625..2515)
                     /gene="pstA"
                     /locus_tag="ACHK0Z_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU4503407.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2515..3474)
                     /gene="pstC"
                     /locus_tag="ACHK0Z_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             complement(2515..3474)
                     /gene="pstC"
                     /locus_tag="ACHK0Z_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU4503408.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3560..4600)
                     /gene="pstS"
                     /locus_tag="ACHK0Z_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             complement(3560..4600)
                     /gene="pstS"
                     /locus_tag="ACHK0Z_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU4503409.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4914..6743)
                     /gene="glmS"
                     /locus_tag="ACHK0Z_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             complement(4914..6743)
                     /gene="glmS"
                     /locus_tag="ACHK0Z_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU4503410.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6905..8275)
                     /gene="glmU"
                     /locus_tag="ACHK0Z_RS17230"
                     /old_locus_tag="pgaptmp_003446"
     CDS             complement(6905..8275)
                     /gene="glmU"
                     /locus_tag="ACHK0Z_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU4503411.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8626..9045)
                     /locus_tag="ACHK0Z_RS17235"
                     /old_locus_tag="pgaptmp_003447"
     CDS             complement(8626..9045)
                     /locus_tag="ACHK0Z_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU4503412.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="ACHK0Z_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="ACHK0Z_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU4503413.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="ACHK0Z_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="ACHK0Z_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU4503414.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="ACHK0Z_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="ACHK0Z_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU4503415.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="ACHK0Z_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="ACHK0Z_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU4503416.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="ACHK0Z_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="ACHK0Z_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU4503417.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="ACHK0Z_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="ACHK0Z_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU4503418.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="ACHK0Z_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="ACHK0Z_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU4503419.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="ACHK0Z_RS17275"
                     /old_locus_tag="pgaptmp_003455"
     CDS             complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="ACHK0Z_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU4503420.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3812 a         4328 c         3965 g         3780 t
ORIGIN      
        1 attgggattt gtctggtgaa ttatttgtcg ctatctttcc ccgccagcag tttatccagc
       61 tcatcgccac cgacgtgacg gaaatcctgc cccttcacgt agtagaagat aaactcacaa
      121 atattctggc agcggtcgcc aatacgttcg atagaacgcg cgcagaacag cgcagtaagt
      181 acgctcggaa tggtacgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
      241 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
      301 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
      361 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
      421 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
      481 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
      541 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
      601 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
      661 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
      721 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
      781 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
      841 caatcaattc gcccaggtac ataaacgccg tgtggtcgga acaacgcgca gcctgctgca
      901 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
      961 tacgcccggt agagataggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     1021 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     1081 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     1141 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaaag
     1201 cgatgttgtc gtagatggac atcggaaacg gcgtcggttt ctggaacacc atgcccactt
     1261 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     1321 tttcaccttc tgcgcgctgc tccggataca gttcaaacat tttgttgaag gtacgcagca
     1381 gcgtcgattt accgcagccg gacggcccga taaacgccgt tacctggttt ttagcgatat
     1441 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     1501 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     1561 tgcttttcgc taatcaggct taccgtaggc cgggtaaggc gttcacgccg catccggcaa
     1621 agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagga
     1681 cgcacagggt aatgatcaat accccggccc aggccagttg ttgccattcc gcaaacgggc
     1741 tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc atgtccgtgc
     1801 tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg gcaatacggg
     1861 caatcgccag caggataccg gtcataatcc cggacaccga cgctttcagc gtaatcgcgg
     1921 agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg ctgtccggca
     1981 ccagtttcag catgttctcg gtggtgcgga taacgatcgg cacctgcaac agcgccagcg
     2041 caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg gtgtagacaa
     2101 acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag cgaatcactt
     2161 ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata atgcccagcg
     2221 gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg gcgttcgcca
     2281 gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc agcgacatac
     2341 cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac aggccgaagg
     2401 ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca
     2461 ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata gccattagcg
     2521 tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga ggacgatgaa
     2581 ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca gaccggattc
     2641 cgcttccgca aattcgttcg ccagcgcaga ggtgatactg ttgcccggca tatacagcga
     2701 ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg tttcaccgag
     2761 cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga acggaagaac
     2821 gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact ctttcatcat
     2881 caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa taatcatgat
     2941 ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag agaacagcgc
     3001 gccaacaatc gggatattcg acatgatatt gccgaccggc tcctggaagt aaacggcgaa
     3061 tagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa tggctgccag
     3121 cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa
     3181 cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg aagtcaccag
     3241 cgtaccgtag atcggcacta gcgccccgta gatatcgttc ggtgcatccc actctttggt
     3301 ccagaggaaa gccagaccaa atttctgaat gctcggccag gaggagatga tcagagagac
     3361 aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca gcacgctgaa
     3421 aattatgtcg ccctttttac ccggtgggtt aaaagcaggc ttggttgcag ccataagtta
     3481 ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggataagcc gttaatgcct
     3541 tatccggcct ggagttttat tagtacagcg gcttaccgct actgtcttta atattggtct
     3601 tccacgcagc gcgaacctgt tcaactacac tatccggcag gctggcgtaa tccaggtcgt
     3661 tcgcctgttt agcaccggtt ttgtacgccc agtcgaagaa tttcagcact tctgtgcctt
     3721 gttctggttt cttctgatct ttgtggatca gaatgaacgt ggtagaggta ataggccacg
     3781 catcttcgcc tttctggttg gtcagatcct gagcgaaggt tttgctccag tctgcgcctt
     3841 ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg tttaccatca gcggagatca
     3901 gtttggtata cgccaggttg ttctgcttcg cgtaagcata ttcaacgtaa ccaattgcac
     3961 ccggcagacg ctgaacgaac gcggcgatac cgtcattacc tttaccgccc agaccgatcg
     4021 gccattttac ggtagagcca gtaccaacgt tgtttttcca ctcttcgttc actttcgcca
     4081 gatagctggt gaagacgaag gaggtaccgg agccatctgc gcggcgtacc acggcgatgt
     4141 tctgtgacgg cagtttcaga cccggattca gtttggcgat ggcttcatca tcccacttct
     4201 tgattttgcc gaggtagatg tcgccgaggg ttttaccatc cagcaccagt tcgccagact
     4261 tcagccctgg aatgttaaca gccagcacca cgccgccaat cacggtcggg aactggaaca
     4321 gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc agaggcacca aaatcaacgg
     4381 tattagcgat aatctgtttt acgccaccgg aagaaccgat accctggtag ttaactttat
     4441 taccggtttc tttctggtaa gtgtcagccc atttggcata caccggcgca gggaaggttg
     4501 cacctgcacc tgtcaggctt gcttctgcaa acacagagaa agcgctcatc gataaggtcg
     4561 cggcgacaac agttgcgacg gtggtacgca taactttcat aatgtctcct gggaggattc
     4621 ataaagcatt gtttgttggc tacgagaagc aaaataggac aaacaggtga cagttatatg
     4681 taaggaatat gacagtttta tgacagagag ataaagtctt cagtctgatt taaataagcg
     4741 ttgatattca gtcaattaca aacattaata acgaagagat gacagaaaaa ttttcattct
     4801 gtgacagaga aaaagtagcc gaagatgacg gtttgtcaca tggagttggc aggatgtttg
     4861 attaaaaaca taacaggaag aaaaatgccc cgcttacgca gggcatccat ttattactca
     4921 accgtaaccg attttgccag gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg
     4981 acatggtaag ccagcagctg cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc
     5041 acatgcggca tctcgatgat gtgcatgtta tcgctactta caaaacccgc atcctgatcg
     5101 gcgaagacat acaactgacc gccacgcgcg cgaacttctt caatgttgga tttcagtttt
     5161 tccaacagtt cgttgttcgg tgcaacgacg ataaccggca tatccgcgtc aatcagcgcc
     5221 agcgggccgt gtttcagctc gcctgcagcg taggcttcag cgtgaatgta agagatctct
     5281 ttcagtttca atgcgccttc cagcgcgatt gggtactgat cgccacggcc caggaacagc
     5341 gcgtgatgtt tgtcagagaa atcttctgcc agagcttcaa tgcgtttgtc ctgagacagc
     5401 atctgctcaa tacggctcgg cagcgcctgc agaccatgca cgatgtcatg ttcaatggag
     5461 gcatccagac ctttcaggcg agacagcttc gccaccagca tcaacagcac agttaactga
     5521 gtggtgaatg ctttagtgga tgccacgccg atttccgtac ccgcgttggt catcagcgcc
     5581 agatcggatt cgcgcaccag agaagaaccc ggaacgttac agattgccag cgaaccaagg
     5641 taacccagct ctttcgacag acgcaggccc gccagggtat ccgcggtttc gccagactgt
     5701 gacaaggtga tcatcaggct gttacgacgc acggcagatt tgcgatagcg gaattcagag
     5761 gcgatttcga cgtcgcacgg aatacctgcc agcgattcaa accagtaacg ggaaaccata
     5821 ccggagttat aagaagtacc acaggcgagg atctgaatat gctcaacctt cgacagcagt
     5881 tcgtcggcgt tcggtcccag ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta
     5941 agggtgtttt tgatcgcgtt cggctgttcg tagatctctt tctgcatgta gtgacggtaa
     6001 atacctttat cgcccgcgtc atattgcaga ttggattcga tatcctgacg ttttacttcc
     6061 gcgccagttt tatcgaagat gtttaccgaa cggcgagtaa tttccgcaat atcgccctct
     6121 tcaaggaaga taaagcgacg ggtcaccggc aacagcgcca gctggtcaga agcgataaag
     6181 ttttcgccca tacccaggcc aatcaccagc ggactaccag aacgtgccgc cagcagggta
     6241 tccgggtgac gggagtccat gatcactgta ccgtacgcac cacgcagttg cgggatagca
     6301 cgcagaacag cctcacgcag agtaccgccc tgtttcagct cccagttcac cagatgggca
     6361 attacttcgg tgtctgtttc agaaacgaag gtatagccac gcgcttttaa ctcttcgcgc
     6421 agcggttcat ggttttcgat gatgccgtta tgcaccacca caatgtgttc agaaacatgc
     6481 ggatgcgcat tcacttctga aggttcaccg tgggtcgccc agcgagtatg agcaataccc
     6541 gtaccgccat gcagaggatg ttcttccgct gcctgagcca gcatctggac tttaccgagg
     6601 cgacgcaggc gggtcatatg accttccgca tcaacaacgg ccagaccggc agagtcatat
     6661 ccgcggtatt ccagacgacg taaaccttca agaaggattt ctgctacatc acgttgcgcg
     6721 atcgcgccaa caattccaca catagttttt gattccgatt tatatcgttg tcggtcaacc
     6781 tgtatgcccg ttttttcggg cgccccgagc cttgtagaga gtggggttat ttttatagtt
     6841 actgcttgtg ggagggagat tatgttatct cctcatccca tgtgaccgga ttacccggcc
     6901 agaatcactt tttctttacc ggacgacgcc agccttcttt ctgagtctgc ggcacacggc
     6961 tgatagctaa tgcattttcg ccgacattac gcgtcacagt tgtacccgca gcaatggtcg
     7021 cgcctttgcc tactgttacc ggggccacca gctgagtgtc ggaaccgaca aacacatcgt
     7081 cgccgataat ggtcttaaat ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc
     7141 cgatgttaac gttatcgcca atttccgcat cgcccaggta agtcagatga ccagctttcg
     7201 agcctttacc cagacgcgct tttttcatct caacaaagtt accgacgtgt gcaccttcca
     7261 gcaactcagc accaggacgc agtcgggcaa acgggccaat agtacaggcc gctgccagat
     7321 tcgcgtcttc cacaacggta tacggactga tttcgcaatc atcgccaatc acgctgtttt
     7381 taatcacgca accggtgccg attttcacgc gatgaccgag agtcacgttg ccctcgatga
     7441 taacgttagt atcaatttca acatcgcgcc cgtgagtaag cgtaccgcgc aaatcaaaac
     7501 gcgccggatc gcgcagcata acgcctgcta acagcagttt ttcagcctgt tcggactggt
     7561 aaacgcgctc cagacgggag agttgcaggc ggttattcac accttctact tcgcttaaac
     7621 gttgcggatg aacggcgacg atttcacgcc cttcctgata cgccagcgca ataatgtcgg
     7681 tgatgtagta ttcgccctga gcattattgt tggtcagctt cgccagccag cgtttcatat
     7741 ctgcgccgtt agcgatcaga atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg
     7801 tggcatcttt gtgctcaaca atgccggtaa ctttgccatt ttcacgggtg atacgtccat
     7861 aaccggtcgg atcatccagt ttcaccgtca gcagaccaat gccaccctgc ggtttagcat
     7921 cacgcaggcg ctggagtgtt tcgacagaga tcagcggcac gtcgccgtag agcattaaaa
     7981 tgtcttcatc atcggcaaag aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct
     8041 gctctgcctg aagcacccag ttaaggttgt cgtctttcag cgcctgttta agcagatcgc
     8101 cgccgtgacc gtacaccagg tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga
     8161 catgctgaac catcgctttc ccggcaaggg tatgcagcac tttcggaaga tcggaataca
     8221 tgcgcgtgcc tttgcctgcg gcaaggatca ctacgctcat agcattattc aacatacgcg
     8281 tcctgactgt aatttgagaa cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt
     8341 tcatcgtgaa atggacagag gataaattgt tcaataacgg attatccccg ctgacgaaag
     8401 tgccattttc gaccatcgtc acgccttttg tctgcagaaa attaaggcag taccttcatt
     8461 taagcgtcag gtggatgttt ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg
     8521 gtgttttata tcagcaggat ctatgtgaac gctcttcagg acggatcacg cgtaaaaaaa
     8581 agccagcctg tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt
     8641 ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc
     8701 gtaatctacg tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat
     8761 ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg tcggccagaa cggtcacgtt
     8821 gccaggctgc acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg
     8881 tttcacgatg cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta
     8941 gatccccagt tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat
     9001 ttgttgctct gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta
     9061 aggcgttaaa gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac
     9121 gcctgctccg gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg
     9181 tctttcaggg agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc
     9241 tgggacagga agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac
     9301 agttcatcca tacccaggat ggcgatgatg tctttcagtt cctgataacg ttgcaggatg
     9361 gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga
     9421 cggctggtgg agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg
     9481 ctcagtacca cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag
     9541 tcatccgcag gtacgtatac tgcctgtacg gaggtgatag aaccagtttt ggtggaggtg
     9601 atacgttcct gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa
     9661 ggcatacggc ccagcagtgc ggatacttcc gtacctgcca gggtgtaacg atagatgttg
     9721 tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg
     9781 gtcagagcaa cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat
     9841 actttgtcga taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta
     9901 cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaatg
     9961 agctccatca tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg
    10021 cccttagcga acggacacat caggtcgata actttgatac cggtttccag cagttcctga
    10081 gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca
    10141 ccgatctcgc ctttcatgtc gaccggttca cccagtacgt tcatgatacg gcccagagtc
    10201 gctttaccta ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc
    10261 agaccgtcgg aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga
    10321 acttccagca ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt
    10381 acggcatcct gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca
    10441 gtagccatct taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga
    10501 tctcggtgag ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct
    10561 ctttaatcag gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct
    10621 gctcgctggc caggttttca accacgccct gataaacctg agattcgaca taacgacgca
    10681 gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt
    10741 tcagatcatc atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag
    10801 acatggtgtt aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt
    10861 aggcctgcaa catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca
    10921 tgccggtgac ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg
    10981 agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca
    11041 gcagtttttt gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca
    11101 ccaggtagcc cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat
    11161 taccgtgtgc aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg
    11221 ccatgcgatc ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga
    11281 tcttttgcgt gttctggaca cttgcgatct tactacgtat ctcttttgcg ccggccatga
    11341 gcttctcctc aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg
    11401 ggttgctttg aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc
    11461 accggtctgg ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag
    11521 agcggcttcg aagctgccaa ttttcgacag ttcaacatcc gccaggtaac cacgttctgc
    11581 tgcgaacaga accagagact gctgcgcaac ggacatcggc gcatactgtt tctgtttcag
    11641 cagttcggtc actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga
    11701 tgcaaactga gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc
    11761 ggacagtttt ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc
    11821 cgggttaacc gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc
    11881 atcggtaatg gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc
    11941 gataatcggc agtgcggtca gagaaccggt tttccctttc acttcacctt tggtgaaggc
    12001 ttcaacgtat tcggcgttaa cacgtgcagc acgctccagc agacgagagt ggaggtagaa
    12061 aacgtcgccc gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg
    12121 gtaagcaaca gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc
    12181 acggaagtat tcgcccattg cgcaaccggc atacggtgcc aggtattgca gtgcagcgga
    12241 ttcagacgcg gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt
    12301 acgtaccacg ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat
    12361 accggaatcg cgctggttga tgatggcatc gatagccagt gcggttttac ctgtctgacg
    12421 gtcaccgatg atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt
    12481 ataaccggtc tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc
    12541 ttctacagca gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag
    12601 agtgttaacc acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt
    12661 acacttaact ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac
    12721 agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc
    12781 accctgcata caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat
    12841 agtaccttcg ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag
    12901 ttcgctgatt tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc
    12961 tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat
    13021 gataacgcct gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag
    13081 acgtttttcc atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga
    13141 aatgacgtct acctcagcgg tagcctcact cacggcacga aggtgaataa actgctccag
    13201 aacatccggg agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc
    13261 gttttcgtcc agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc
    13321 aagcgcgcca gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc
    13381 cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt
    13441 ggcgtagggg cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca
    13501 gcgacaagtt tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc
    13561 ttctcggcgc cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt
    13621 ttacgctcgg cttcaatttc tgcctgcgcc tgggccacga ttttagtacg ttcctgttct
    13681 gcctcagctt tcgcttcgtc cagaatctgc gagcggcgtt tgttcgcctg ctcgatgatt
    13741 acctgggctt ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca
    13801 aggtccttat gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt
    13861 tcgatggctg ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca
    13921 aacgcgatgg cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta
    13981 gttaacgttc tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat
    14041 cacgtacaga cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat
    14101 aaagaactga gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa
    14161 tttacccccg aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac
    14221 agcggcagcc atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt
    14281 tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga
    14341 cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg
    14401 cccacggcac attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga
    14461 aaatcagctc accggcatac atgttaccga acagtcgcaa accaagtgaa actggtttgg
    14521 acagcaggct taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg
    14581 gctgcagcgt caactctttc gtgaagccgc cgatgccttt cattttgatg ctgtagaaca
    14641 gaatcaggat aaatacgccc aatgccatag acagcgttac gttcacgtcc gcagacggaa
    14701 ccacacgcag tgcaggcaga cccagtacat gttcagcaat gtacggcagc aggtcgatag
    14761 gcagtaaatc catcaggttc atcaggaata cccagacgaa gatcgtcagg gccagcggag
    14821 caatcagctt gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca
    14881 ccagctcaat cgcggtctga aacttacctg gcacaccgct ggtcgccttt ttggctacgc
    14941 tacggaataa aaccaggaac aacagaccca gcaccaccga gaagaacatg gagtcaatat
    15001 tgattgtcca gaaggtggct ggggggtttt gtggatccac cagcgagaat gtacgcaggt
    15061 ccagctgaag gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag
    15121 ccatgatgcc ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag
    15181 caccaaaacc cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac
    15241 caccagtaac accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc
    15301 cacccggcct ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac
    15361 gttaggcaga aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt
    15421 gaggctgaac agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg
    15481 agcaacgttt cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga
    15541 ggtatgccgc gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg
    15601 tcaaattata cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat
    15661 gtttaaatat ttttccagtg catacaattg cgacttttct gctaaccctg ttcgatcatg
    15721 aaaaactgta aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg
    15781 catgttcgcg cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata
    15841 tttaagtcta aaaacagata ctgttttaat aaatgacatt tacac
//
LOCUS       DAZFWP020000111        15814 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFWP020000111 DAZFWP020000000
VERSION     DAZFWP020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.111"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            96..638
                     /gene="yfaZ"
                     /locus_tag="ACHK0Z_RS17280"
                     /old_locus_tag="pgaptmp_003456"
     CDS             96..638
                     /gene="yfaZ"
                     /locus_tag="ACHK0Z_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU4503421.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     GGNRDNAVADGPYVGVNASF"
     gene            738..1940
                     /gene="yfaY"
                     /locus_tag="ACHK0Z_RS17285"
                     /old_locus_tag="pgaptmp_003457"
     CDS             738..1940
                     /gene="yfaY"
                     /locus_tag="ACHK0Z_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU4503422.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2004..2465
                     /locus_tag="ACHK0Z_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /pseudo
     CDS             2004..2465
                     /locus_tag="ACHK0Z_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2506..3408)
                     /gene="rpnE"
                     /locus_tag="ACHK0Z_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             complement(2506..3408)
                     /gene="rpnE"
                     /locus_tag="ACHK0Z_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4503423.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3602..4792)
                     /gene="glpC"
                     /locus_tag="ACHK0Z_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             complement(3602..4792)
                     /gene="glpC"
                     /locus_tag="ACHK0Z_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU4503424.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4789..6048)
                     /gene="glpB"
                     /locus_tag="ACHK0Z_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             complement(4789..6048)
                     /gene="glpB"
                     /locus_tag="ACHK0Z_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU4503425.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6038..7666)
                     /gene="glpA"
                     /locus_tag="ACHK0Z_RS17310"
                     /old_locus_tag="pgaptmp_003462"
     CDS             complement(6038..7666)
                     /gene="glpA"
                     /locus_tag="ACHK0Z_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU4503426.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVAAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7939..9297
                     /gene="glpT"
                     /locus_tag="ACHK0Z_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             7939..9297
                     /gene="glpT"
                     /locus_tag="ACHK0Z_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU4503427.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9302..10378
                     /gene="glpQ"
                     /locus_tag="ACHK0Z_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             9302..10378
                     /gene="glpQ"
                     /locus_tag="ACHK0Z_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4503428.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10420..10659)
                     /locus_tag="ACHK0Z_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /pseudo
     CDS             complement(10420..10659)
                     /locus_tag="ACHK0Z_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10657..10761
                     /locus_tag="ACHK0Z_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /pseudo
     CDS             10657..10761
                     /locus_tag="ACHK0Z_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10841..11491
                     /gene="inaA"
                     /locus_tag="ACHK0Z_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             10841..11491
                     /gene="inaA"
                     /locus_tag="ACHK0Z_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU4503429.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11545..11799)
                     /gene="yfaE"
                     /locus_tag="ACHK0Z_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             complement(11545..11799)
                     /gene="yfaE"
                     /locus_tag="ACHK0Z_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU4503430.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11799..12929)
                     /gene="nrdB"
                     /locus_tag="ACHK0Z_RS17345"
                     /old_locus_tag="pgaptmp_003469"
     CDS             complement(11799..12929)
                     /gene="nrdB"
                     /locus_tag="ACHK0Z_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4503431.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13076..15361)
                     /gene="nrdA"
                     /locus_tag="ACHK0Z_RS17350"
                     /old_locus_tag="pgaptmp_003470"
     CDS             complement(13076..15361)
                     /gene="nrdA"
                     /locus_tag="ACHK0Z_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4503432.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15489..15632
                     /gene="ypaB"
                     /locus_tag="ACHK0Z_RS17355"
                     /old_locus_tag="pgaptmp_003471"
     CDS             15489..15632
                     /gene="ypaB"
                     /locus_tag="ACHK0Z_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU4503433.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         3675 a         3904 c         4311 g         3924 t
ORIGIN      
        1 tagatccctt ttttcgctca ttgaatcctc tgtgccaggc agtacaatcc cgcagctttg
       61 ggaattttga ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc
      121 tggccggaat gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg
      181 gtaaagaata caccaatatt ggtgtcggtt ttgggactga atcgacgggc ctggctttaa
      241 gcggtaactg gacacataac gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga
      301 atctgcctct cgggccgtta atggcgaccg ttggcggaaa aggcgtttac accaacccga
      361 attacggcga tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg aaaattggca
      421 acagcttccg tttgtttggc gagtattact actctccgga ttcgctctcc agcggtattc
      481 aaagttatga ggaagcgaat gctggcgcgc gttacaccat tatgcgtcca gtcagtattg
      541 aggcgggtta tcgctacctg aatctgtcgg gtaaaggcgg taaccgcgac aacgccgtgg
      601 ctgacggccc gtatgttggg gttaacgcca gtttctgatc ccttccggcg cggcattctg
      661 tcgcgccatt cagtttctta ctacgcctta tctccctttg cgttatagtg attcgataac
      721 aaaagcagga gataaccatg ttaaaagtgg aaatgctatc caccggggat gaagtgttac
      781 acgggcaaat cgttgacact aacgctgcct ggctggccga ttttttcttt catcaggggc
      841 tgccattatc tcgccgcaat acggtggggg ataaccttga tgacttagtc accattcttc
      901 gcgaacgtag tcagcacgcc gatgtgctga tcgttaacgg cgggctggga ccgaccagcg
      961 atgatttaag cgcactcgcc gctgcgacag caaaaggtga aggcatggtg ctgcatgaag
     1021 cctggctcaa agagatggaa cgctatttcc acgaacgtgg acgagtaatg gcaccgagca
     1081 accgtaaaca agcggagctg cctgccagtg ctgaatttat caataacccg gtaggcaccg
     1141 cctgtggttt tgccgtgcag cttaatcgtt gcctgatgtt ctttactccc ggcgtaccgt
     1201 cagaatttaa ggtgatggtc gagcacgaaa tcctgccgcg tctgcgcgag cgtttttctt
     1261 taccacagcc gccggtttgt ctgcgtttaa ctacctttgg tcgttcggaa agcgatctgg
     1321 cacaaagcct ggacactcta caactgccgc cgggcgtaac aatgggctat cgctcttcaa
     1381 tgccgattat cgaactgaaa ctcaccggac cggcaagcga gcaacaggcg atggaaaaac
     1441 tgtggctgga cgttaaacgt gttgccggac agagcgtgat tttcgaaggc accgaaggac
     1501 tgcccgcgca gatcagtcgc gaattgcaaa accgccagtt cagcctgacg ttgagcgagc
     1561 aattcaccgg cggtttattg gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt
     1621 gtgaagtggt tccttcacag gaagaaaccc tggcgcaaac tgcgcactgg attacagaac
     1681 ggcgggccaa ccattttgcc gggctggcac tggctgtttc gggtttcgag aacgagcatc
     1741 tcaactttgc gctagccacg ccagacggca ctttcgctct gcgtgtgcgt ttcagcacta
     1801 cacgctacag cctggctatc cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc
     1861 gccgttggtt aaacggccag gatatcgcca gtgagcatgg ctggattgag gttattgagt
     1921 ccatgacctt atctgtctga ttccgccact ctgtgccggt ttactgcacc ggcacattcc
     1981 ccctccggca aaacttgtcc aaccctccct acggtgagcg tggtgttggg gccagtgtgg
     2041 cacgggctgc gcgctgggga cgcattgaga attacatggc gcaagttaac gattcacttt
     2101 gtctgttggt gcaggtggaa agtaaaacgg cactggataa cctggacgaa atcctcgacg
     2161 tcgaagggat tgatggcgtg tttattggac ctgcggatct ttctgcgtcg ttgggctacc
     2221 cggataacgc agggcacccg gaagtgcagc gaattattga aaccagtatt cggcggatcc
     2281 gtgctgcggg taaagcggct ggttttctgg ctgtggctcc tgatatggcg cagcaatgcc
     2341 tggcgtgggg agcgaacttt gtcgctgttg gcgttgacac gatgctctac agcgatgccc
     2401 tggatcaacg actggcgatg tttaaatcag gcaaaaatgg gccacgcata aaaggtagtt
     2461 attgatatca aaggcccatg gggatcggct gtgggcctgt gttaattagt ggttattcgc
     2521 tgccagatcg gcttcgctta gctgggtggc cgcgagcacc tggtcacgat caatgccctg
     2581 ttccagcatg cgcaaagcaa ttttaatggc ttgttcatgc ataccttcct gccagccaat
     2641 ttgatgccct tcctgccgta gccgttcagc aatagtcatt aatctctcct tttgtagtgg
     2701 tgaacgttcg gcaatctcct caataaaacg ggtgaaacgg gaggtatcgc cgcattgcag
     2761 cagataatta aacagtgttt gtagctggct gtcattagtg aagcctctaa ccaaaagcgt
     2821 ggtgatcctg tcgaccatgc cgattaaatc gcggtcgcga atatgctttt gaatcagttc
     2881 cagcagagct atacgccgat gttgcatgat ctcatcgtca ggtacgatgg tgatatccac
     2941 caacggaaaa gcttcggtgt acaactgccg ggcaagttgc ggatcgtcaa actcatccag
     3001 ccagttaagt gagtacgggt agggcgatgt ttcgccatga taaaacagca atggcaccac
     3061 cagcggaact ctgtcatagc ctttatccag gtgacgctgc atggcggcag tggcatagcg
     3121 cattagccga aaagccatat tcttttctgc agagctttga tgttcaatca cgcagtagat
     3181 atagccgtca ccgtcgctgg tttccacgga ccacaaaaca tccgagtagt aagcgcgtaa
     3241 acttttttca ataaaactag tgggttccag gcgtaaggtt tgcaggttgc aaagcttgcg
     3301 cagtggttct ggtaaatgta tttcgagaaa atcccgtgcg gtttcgggtg tgaacataaa
     3361 ggttttaaac accgcatcat gcggggagga tgttgttgat tcggtcatgg ttgtccgtca
     3421 cctgatattt atgatgacgc gacattacgt ctatcccggg cctttgacat ctgtcagttt
     3481 cagcctttgc gagacatttt cagaggaatt ttcaaagcgt tgcaacgcat acatggtgcg
     3541 tggaatcgcg ctcgcaaaaa tgcccgataa gcaatgtgct taccgggcat caaaaggtcg
     3601 gttaagccag cgcctgggcc agtagcgtaa tcggatgttc gcagcgaagg ctcgtggaca
     3661 tctcaatctg ccatttgcag gtttcgcagt cggtgatgac cagatccgcg ccgctctctt
     3721 ctatctggcg gaacagtggc gcaccgatgg cttgtgaggt ggggtagttc tcttttttga
     3781 aaccgtaagt acccgcaata ccgcagcact gggaatccag caccgttaac tcaagccccg
     3841 ggattttacg caacagctcc agggtgtaga gcgtccagcc cattttttcc atatggcacg
     3901 gagtgtgata aaccactttc agcggcagcg gtttcagcgg taacgttttg ccttcgtcca
     3961 gcttgcgcca cagccagcgg gttgccagtt cgatatgatc gcgcaagcct ttgttgtcga
     4021 cattcagcac ttccgggtat tcgtcgcgca gggcaaatgt acaggttgag gaggtggcaa
     4081 tcaccggaat tccttttact cccacagctt cgcggatcga ctctacattc gtaattgcct
     4141 gtttgcgtgc tttatcggta aagccgttgg cgattagcgg tacgccgcag catttttctt
     4201 tgctgagcag ttgtacaccg gtacccattg cgttgagcac tttaattaaa tctttaccta
     4261 actgcggatg gttgtagtta acgaagcagc cgtgaaagaa agcgacctgg tctttatatt
     4321 gtgcttgctg agccgctatg ctgcgatacc agcgacggaa cgtgccgaag gagtatttcg
     4381 gtagcgtgcg gcgatgatcg atttttaacg ccgcatcaag cagctgccgc accggtttca
     4441 gcgaggtagc ggtgttgacg attggtgcga acggcgtgga aacgctaccc atcaggtcgg
     4501 tatgactcaa cacaaaatta cgcagcgacg ggcgcgtggt gtcatatttt gcccgcgcgc
     4561 gctggataat atcgccaatc ttcacatcgg acgggcaggc gacttcacaa cgtttgcagt
     4621 tgatgcaata tttcagcgcc tcgtcataca gtgcgccatc tttcaaacgc agacgctcgc
     4681 catccggccc ggcttgtttt ggccctggat aaccgggatt cacccggctc accgggcagg
     4741 cggtggtgca gacggtgcac ttaatgcagt tttcgaagct ggtgtcgttc attgttggcc
     4801 tcctgcgcgt tgggcaatct gttgagcggc atgtaaagca ctgacggcac aaacaccgcc
     4861 gccgcatccc tgggcgatgg gatcaaatcc gcccagcacc gaaccgatgg caaacaggtt
     4921 ctcaatggtt tgccctgcct gcgacgggcg tagcgtctca tcagtggtta caccgaactg
     4981 ctgccacggt tgcggcgcaa aaaaatcgcc cttataccat tcaccccgcg tggcggtttg
     5041 tagcacatca aggccgagaa tcggctcgcg aatgccgtta cgttccgcta ccagtccgcc
     5101 actaaagaaa ctgccgctgg cgagaaccgc gaaacgtgga cgtagcggaa tatcggcgtg
     5161 attgcgggtc cagatttcat tcactacgcc atttttacag gtcacttttt tcacttcatc
     5221 gcccggcatc cacacgccac cctggcgcac aaactggcgc tgtaactggt tttgcagacg
     5281 aatgcccagc acggaaggcg gcagcgttgg caaaagcatc agtgaacaag gtagtttttc
     5341 attcaaccaa cgccacagtt tgtcatcggc caggccgaag caggcgggca tcaggatcat
     5401 ttcgcaggta ttggcgacag gaataagcgc atcaagtaac agcggccagt tttcttcatt
     5461 atcaaggaaa cgggcgatat tcacagcgcg aaattcggtg gcgttattgc gcaggacatc
     5521 cagttccggc agctctattt ctgcggtttc aaccgaaaga tccagttcgc gcaacgaagc
     5581 cgccgcaaga tgcgcctgga aatccatcag cccgcttatc cccactacac atatcttctt
     5641 cgcgggtagc ggccagacgg gaacttctgg cgaacttaac caggttgcac gcaaagtgcc
     5701 gagcggggta acgcgctgat gggccagttc cacgctgccc tgcaattgcg caccgctttc
     5761 agcgatcaat gcctgcgcct ggcaagcgag atcgagcacg cgttgtggtt cgagaagact
     5821 gtaaggatgg gctggtgcct gctgacgcaa agattccagt ccactgtgaa tgtctgtcac
     5881 cggttgacca tcaggcagat ggctcagcaa atccagcgaa ccggatgaga aatgcagcgc
     5941 gctttgacca cgcgtgacaa tggcacagcg caggccgtgt ttttgcagtt gcaggccaca
     6001 gagtaatccg gcgaggccgc cgcccataat gacagtatca aagcgcatct ttctgctcct
     6061 tctccagacc acacagtccc tgataaaccc agcgggtaaa ttcgctttcg cgcagtgcgt
     6121 ctccccaggc gatgggttgc acgcctttcc agcgttcgtt aaggaaggtg gaaagttgct
     6181 cgatagattg cgcggacgtc gtgacgttaa aacgttgcag cagtccggca gcgcggcagg
     6241 cgcagagttc gccctggcag gtacccatcc ccacacgggt acgacggcgt aaatccagca
     6301 ggctattaac gtttaaattt tctaccgcgt actgaacttc acccgcagtt accgcttcgc
     6361 actcacatac caggctacga tgcagacggc cttcgctcag ccaggcaggc gtgcgatcgc
     6421 catgacgata aaccgcagaa ccgcgcagcg gggcaggcag ggagatgact ttacgcaagg
     6481 taacttcagc gggttcttgt gaaccaggca gtgccagatc ggcagtcgta caggggcgcg
     6541 tgttgcccag tttgcggcat accgcgtcgg tagcccattc agccatcagc cgataggtca
     6601 tcagtttgcc accggtgatg gtgataaatc cgtccagacc atcgcgttca gcatggtcga
     6661 gcagcacgat gccacggctg acgttacgtc cgctcgggtc gtcatcgctg gcaaccagcg
     6721 ggcgcacgcc agaataggcc cgcaaaatgc gcgttttcgc catcacgggg gccagttttt
     6781 ccccttcacg cagcagaata tcaacctctt ctgccgccac tcgattatcg tcaatctcgt
     6841 tgtaatcaat acgtaaagag gtggtgccaa tcagcgaaat ggtatcgcca ggcaccagaa
     6901 tatcggcgtc ggaaggttta cggcagcggt tgatcacatg ctggttaatg cggtgatcca
     6961 tgatcagcag cgatcctttc gccgggaaca tgcgaatgcg cagatcggca tattcggcaa
     7021 tgtgttgccc ccagatccca gcggcattaa ccacgacagg tgcatgaagg gcctgagttt
     7081 cgccggtgag atggttacgt acacgaacac cgcacaccgt cgcgccttca cgaatcagcc
     7141 ccgtgacttc atgagcggta aggataacgg caccgtgttc tttggcatcc agcatgtttg
     7201 ctgcggtcag acgaaatgga tcaacggtgc catccggaac tttcaccgcg ccaatcagtg
     7261 ccgggttaac ggcaggttcg ataatgcgcg cttgctgcgg gtctatagct tctgcgctga
     7321 tccctgcttc ttcgcaggcg cgaataaaag tggcctggaa tgagaggtca tcttccggca
     7381 gggtgataaa caggccgttg gttggttcaa cgcagtgacg tgcaatgcgt ttcaggatct
     7441 ggttttcact aatgcattcg cgggccgatt ccgcatcggt taccgcatag cgcgcaccgc
     7501 tgtgcagcag gccgtggtta cgcccggtgg caccggttgc gatgtcgtgg cgctcaacca
     7561 aaatcacgcg cagcccgcgc agggcacagt cgcgggcaat cccggctccc gttgcgccgc
     7621 cgccaatgat aatcacgtca cttgattgcg agtcgcgagt tttcattgtt atccctctga
     7681 agttcgtttt ttaccattta gccatagtaa aaacatgaat tgtttgattt cgcgcatatt
     7741 cgctcataat tcgaaagtga aacgtgattt catgcgtcat tttgaacatt ttgtaaatct
     7801 tatttaataa tgtgtgcggc aattcacatt taatttatga atgttttctt aacatcgcgg
     7861 caactcaaga aacggcaggt tctctcactg aatcaggctg ttaatcataa ataagaccac
     7921 gggccacgga ggctatcaat gttgagtatt tttaaaccag cgccacacaa agcgcgctta
     7981 cctgccgcgg agatcgatcc gacttatcgt cgattgcgct ggcaaatttt cctggggata
     8041 ttctttggct atgcggctta ctatttggtt cgtaagaact ttgcgcttgc tatgccttat
     8101 ctggttgagc agggattctc acgcggtgat ttaggttttg ccctttcggg gatctcgatt
     8161 gcttatggat tttcgaaatt catcatgggt tcggtatcgg atcgctcgaa tccgcgcgtt
     8221 ttcctgcccg caggtttgat tctggcggcg gcagtgatgt tgtttatggg ctttgtgcca
     8281 tgggcgacgt cgagcattgc ggtgatgttt gtactgttgt tcctctgcgg ttggttccag
     8341 gggatggggt ggccgccgtg tggtcgtact atggtgcact ggtggtcgca gaaagaacgt
     8401 ggcggcattg tgtcagtgtg gaactgtgcg cacaacgtcg gtggtggtat tccgccgctg
     8461 ctgttcctgc tggggatggc ctggttcaat gactggcatg cggcgctcta tatgcccgct
     8521 ttctgcgcca ttctggtggc attattcgcc tttgcgatga tgcgcgatac cccgcaatct
     8581 tgtggtttgc cgccgatcga agagtacaaa aatgattacc cggacgacta taacgaaaaa
     8641 gcggaacagg aactgacggc gaagcagatc ttcatgcagt acgtgttgcc gaacaaattg
     8701 ctgtggtata tcgccatcgc caacgtgttc gtttatctgc tgcgttacgg catcctcgac
     8761 tggtcaccga cttacctgaa agaggttaaa catttcgcgc tggataaatc ttcctgggcc
     8821 tacttcttct atgagtatgc gggtattccg ggcaccctgc tgtgcggctg gatgtcggat
     8881 aaagtcttcc gtggcaaccg tggggcaacc ggcgttttct ttatgacact ggtgaccatc
     8941 gcgactatcg tttactggat gaacccggca ggtaacccaa ccgtcgatat gatttgtatg
     9001 attgttatcg gcttcctgat ctacggtcct gtgatgctga tcggtctgca tgcgctggaa
     9061 ctggcaccga aaaaagcggc aggtacggca gcgggcttta ccgggctgtt tggttacctg
     9121 ggcggttcgg tggcggcgag cgcgattgtt ggctacaccg tggacttctt cggctgggat
     9181 ggcggcttta tggtaatgat tggcggcagc attctggcgg ttatcttgtt gattgttgtg
     9241 atgattggcg aaaaacgtcg ccatgaacaa ttactgcaaa aacgcaacgg aggctaatgg
     9301 catgaaattg aagctgaaaa accttagcat ggcgatcatg atgagcacta tagtcatggg
     9361 aagcagtgca atggcggcgg acagcaacga aaaaatagtc atcgcccatc gcggtgccag
     9421 tggatatttg ccggagcata cgctgccagc aaaagcgatg gcgtatgcgc agggagcgga
     9481 ttatctggaa caggatttgg tgatgaccaa agacgaccat ctggttgttc tgcatgacca
     9541 ttatctcgat cgtgttactg atgttgccga tcgtttcccg gatcgggcgc gcaaagacgg
     9601 tcgttactac gcgatagatt tcacgctgga tgaaattaag tcgctgaaat ttaccgaagg
     9661 tttcgatatt gaaaacggta aaaaagtaca gacttatccg gggcgtttcc caatgggtaa
     9721 gtccgacttc cgggtgcaca cctttgaaga agagattgaa tttgttcagg ggttaaatca
     9781 ctctaccggg aaaaatatcg gtatctatcc agaaattaaa gcgccgtggt tccatcatca
     9841 ggaagggaag gatattgcgg caaaaacgct ggaagtgctg aagaaatatg gttacaccgg
     9901 taaggacgac aaagtttatt tgcaatgttt tgatgctgat gagctgaagc gtattaagaa
     9961 tgagctggaa cccaaaatgg gcatggatct caatctggta cagctgattg cctataccga
    10021 ctggaatgaa acgcagcaga aacagccgga tggaagctgg gttaattaca actacgactg
    10081 gatgtttaag ccgggtgcta tgaaacaggt ggcggaatat gccgacggta tcgggccgga
    10141 ttaccatatg ttgattgagg aaacatcgca gccgggtaat atcaaactca ctggcatggt
    10201 gcaagatgct cagcagaata aactggtagt gcatccttat accgtgcggt cagataaact
    10261 gcctgaatac actactgatg tgaatcagtt atatgatgct ctgtataaca aagcgggtgt
    10321 aaatgggctg tttactgatt tccctgataa ggcagtaaaa ttccttaata aagagtaatg
    10381 ctactgcagg atggcgttca tgcgccatcc tgtctcacat caaatttttc cattcaccac
    10441 gagttgttcc gtacacgttt gtagtaaaaa cagtggactg ccttctaccg gcttttcttt
    10501 agccaacaac gaaatctgtc gatagaaggt tggttcgtga ggaacggaac acaattcacc
    10561 tgcaatgctt ttcagcgtta actctggttg taaggcgatg cctaatccct gtcgaataaa
    10621 cttcatcgct gtatccgact gattaaattc acattttatc tactgggcgc actattagtg
    10681 gtagtggctg gtggaatcta tgcgctgcaa atgaagggta aaagcggtgt cacgctagta
    10741 gtggcaaaag aaattcatta atacgacacg tttcattaag attttcctca ggtaaccacc
    10801 tatagtcatt gtcgttaata aaaatgacag gcgatagggt atggcagttt cagcaaagta
    10861 cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc ctaactatcg
    10921 ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc tgtatgtaaa
    10981 gcgcatgacg catcatctgt ttcattccgt acgttatccg ttcggccgac caacgattgt
    11041 ccgtgaggtt gcggtaatta aagaactgga acgggccggt gtcatcgtgc cgaagatcgt
    11101 ttttggtgaa gcggtgaaaa ttgagggtga atggcgagcg ttgctggtga ctgaagatat
    11161 ggcggggttc atcagcattg ctgactggta tgcccagcat gcagtatcgc cttattctga
    11221 cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcat
    11281 taatcgtcag catggctgtt gttatgttcg ccatatctat gtgaaaacag aaggtaatgc
    11341 agaagctggt tttcttgacc tggagaaaag tcgtcgccgc ttgcgtcgcg ataaagcgat
    11401 taatcatgac ttccgccagc tggaaaaata tctggagcca atccccaaag cggactggga
    11461 gcaggtgaaa gcgtattact acgccatgta atcgttgtag gccagataag acgcataagc
    11521 gttgtatctg gcactacaac caattcacat ctcgatttca atatcgcctt ttgcccggca
    11581 acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa tccagtcaac
    11641 ctgacctgca accagacggg tgcgacagga gccgcagtaa ccttcgcgac actgatactc
    11701 aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat cctggcacag
    11761 cagttgtgtg ccagtgatgc gcagggtaac gcgggccatc agagttggaa gttactcaaa
    11821 tcgtcggtgt ccacttccgc gtcaatctgc ccgaccagat aagaactgac ttccacttcc
    11881 tgcggagcaa cctgcacgtt atcagacacc agccaggtgt tgatccatgg gatcgggttg
    11941 gagcgcgtct ggaacggcag atccaaaccg actgcctgca tacggatatt ggtgatgtat
    12001 tcaacgtact ggcagagaat gtctttattc agaccaatca tcgaaccgtc gcggaacaga
    12061 taatccgccc agtctttctc ctgttgagct gcctgaacaa acaggtcata gcactcctgc
    12121 ttacactctt cggcaatttc cgccatctca ggatcgtccg cgccgctgcg cagcagattc
    12181 agcatatgct gggtgccggt caggtgcagg gcttcgtcgc gggcaatcag gcgaataatt
    12241 ttggcgttgc cttccatcaa ttcgcgttct gcaaatgcga aggaacaagc aaagctgacg
    12301 tagaaacgaa tcgcttccag cgcgttaacg ctcatcaggc agagatacag ttttttcttc
    12361 agctcgcgca ggctaacggt cacagtttta ccgttaacgg tgtgggtacc ttcgcccagc
    12421 agatgccagt agctggtcat ttcgatcagc tcatcgtaat agctggagat cccttccgca
    12481 cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg atcgttaacg
    12541 atattacgaa tgatatgagt ataggaacgg gaatgaatcg tttctgagaa cgcccaggtt
    12601 tcgacccagg tctccagttc cgggatcgag atcagcggca gcagtgccac gttcgggcta
    12661 cggccctgaa tggaatccag cagcgtctga tatttcaggt tgctgataaa gatgtgtttt
    12721 tcgtgttcag gcagtgcctg gtaatcgata cggtcgcgag aaacgtcaac ttcttccgga
    12781 cgccagaaga aagagagctg cttttcgatc agcttttcga agatgtcata tttttgctga
    12841 tcgtagcgag ccacgttgac cggctgacca aagaacatcg gttctttgag ctgatcattt
    12901 ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct gttgggagtt taatgccgga
    12961 taaggcattt tacgccgcat cctgtgcctg atgcgacgct gacgcgtctt atcaggccta
    13021 caaatccgag ccgtaggccg gataaggcgt tcacgctgca tccggcatct caatatcaga
    13081 tcttacatgc gccgctttcg cagccatcgt cctggattga cggtaccaga tcgtcctgcg
    13141 cgtcttcagc gccgtcacgg gtgttctgat aatacagcgt tttgaccccg aatttgtagg
    13201 cggtgagcag gtctttcagc aactgctgca tcggcacttt tcctgacggg aaacgtgacg
    13261 gatcgtagtt ggtgttagca gagatcgact gatcgataaa tttctgcatg atacctacca
    13321 gttgcaggta gccatcgtta cccggcattt cccacagcag ctcataggcg tcgtgcaggt
    13381 gctcgtagtc cggcaccacc tggcgcaaaa taccgtcttt cgacgctttg atgctgacgt
    13441 aaccgcgcgg cggttcaata ccgttagtgg cgttagagat ctgtgaagaa gtctctgacg
    13501 gcatcagggc agaaagcgtg gagttacgca ggccgtgcgt tttgattgat tcacgcaatg
    13561 cttcccagtc gtaatgcagc ggctcattag cgatggtatc cagatctttc ttataggtat
    13621 cgatcggcag gatccctttc gcgtaagtgg tttcgttaaa ccacgggcac gcgccttgct
    13681 cttttgccag ctcgttagac gctttcagca ggtaatactg aatcgcttcg aaggttttat
    13741 gcgtcaggtt gttggcgctg ccgtcggagt aacgcacacc gtgcttcgcc aggtagtaag
    13801 cgaagttgat cacaccaata cccagcgtac gacgacccat cgctccacgt ttggcggccg
    13861 ggatcgggta atcctgataa tccagcagcg cgtcaagtgc acgaaccgcc agaattgcca
    13921 gctcttccag ttcatccagg ctattaattg cgcccaggtt gaaagcagac agcgtacaca
    13981 gcgcgatttc accgttctcg tcgttgacgt cgttcagcgg tttggtcggc agggctatct
    14041 ccaggcacag gttagactga cgcactggcg cgatggccgg atcaaacggg ctatgggtat
    14101 tgcagtggtc aacgttctga atatagatac gaccggtaga cgcacgttcc tgcatcatca
    14161 gcgagaacag ctcaacggct ttcacacgct gcttgcggat gctgtcgtct ttctcatatt
    14221 tggtatacag gcgttcaaac tcttcctgat cggcgaagaa tgcgtcgtac agccccggta
    14281 cgtcggacgg gctgaacagg gtgatatctt cacctttcag caggcgggta tacatcagtt
    14341 tgttgatctg taccccgtag tccatatggc gcacgcggtt gccttccaca ccacggttgt
    14401 ttttcaacac cagcaggctt tccacttcca gatgccacat cgggtagaac agcgttgccg
    14461 caccgccgcg cacgccgccc tgagagcagg atttcaccgc tgtctggaaa tgtttgtaga
    14521 acggaataca gccggtatgg aacgcttcac caccgcgaat cgggctaccc agcgcacgaa
    14581 tacgcccggc gttgatgccg atcccggcac gctgggaaac gtatttcaca atcgcgctgg
    14641 aggtagcgtt gatggaatcc agactgtcac cgcactcgat cagtacgcag gagctgaact
    14701 gacgagtcgg ggtacgcacg ccggacatga ttggcgtcgg cagcgaaatt ttaaatgtgg
    14761 aaaccgcgtc gtaaaaacgc ttcacatatt gcaggcgcgt ttcacgcggg tagttcgaga
    14821 acaggcacgc ggcaaccaga atataaagga actgggcgct ttcgtagatt tcgccggtca
    14881 cgcggttctg taccagatat ttgccttcca gctgcttaac ggcagcataa gagaaggtca
    14941 tatcacggtc gtgatcgata aacgtgtcca tctgcttgaa ctcttcttcc gtgtagtctt
    15001 ccagcagatg attatcgtat ttgcccatct cgaccatttt caccacgtgg tcgtacagcg
    15061 caggcggctc aaactggccg taggcttttt tacgcaggtg gaagatcgcc aggcgcgcgg
    15121 cgagatactg ataatccggc gcatcacggg agatcaggtc tgcggccgcc ttgataatgg
    15181 tttcgtggat gtcagaggtc ttgataccgt cataaaactg aatgtgggag cgcagctcga
    15241 cctgggaaat cgaaacgtta tgcagtcctt ctgccgccca atccagaacg cgatggattt
    15301 tgtcgagatt gatgcgctct gtgctaccgt cgcgctttgt caccagcaga ttctgattca
    15361 tgtatgtcgt acctgttttt ggaattcttt ccgcaatagt ttcatgcaga ctacctgtag
    15421 taagggaagc tgcttagaac actatatata gggggtatgt ttgggattca ctgcaagata
    15481 gtgtgaaaat ggccctcttg caagtgcata actttgtgga taactcagga aggaaaaagt
    15541 ggctttcgcg caccttaggt cagacaaggt gtccgggaaa gtcaatggga agagaaaaat
    15601 ttgttaaaaa taactgttcg gcgatttctt gaacggcaga ataggttgga taaattgata
    15661 tagatggttt attaatcttt ttgctaatga ttgcaaaaat tgatctaagc acgagtcatc
    15721 acataatctg atgtatcagt tgtggtcaat atataaacga atgtaagcga ttgttgatgt
    15781 cgaaatggtt gttttctatt cggttattgt taaa
//
LOCUS       DAZFWP020000112        15732 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFWP020000112 DAZFWP020000000
VERSION     DAZFWP020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.112"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..882
                     /gene="yieE"
                     /locus_tag="ACHK0Z_RS17360"
                     /old_locus_tag="pgaptmp_003472"
     CDS             1..882
                     /gene="yieE"
                     /locus_tag="ACHK0Z_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU4503434.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1087..2556
                     /gene="lysP"
                     /locus_tag="ACHK0Z_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             1087..2556
                     /gene="lysP"
                     /locus_tag="ACHK0Z_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU4503435.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPH
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2849..4840
                     /gene="cirA"
                     /locus_tag="ACHK0Z_RS17370"
                     /old_locus_tag="pgaptmp_003474"
     CDS             2849..4840
                     /gene="cirA"
                     /locus_tag="ACHK0Z_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU4503436.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4872..5708)
                     /gene="yeiG"
                     /locus_tag="ACHK0Z_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             complement(4872..5708)
                     /gene="yeiG"
                     /locus_tag="ACHK0Z_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4503437.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5966..6634
                     /gene="folE"
                     /locus_tag="ACHK0Z_RS17380"
                     /old_locus_tag="pgaptmp_003476"
     CDS             5966..6634
                     /gene="folE"
                     /locus_tag="ACHK0Z_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU4503438.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6651..7808
                     /gene="yeiB"
                     /locus_tag="ACHK0Z_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             6651..7808
                     /gene="yeiB"
                     /locus_tag="ACHK0Z_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU4503439.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7950..8990
                     /gene="galS"
                     /locus_tag="ACHK0Z_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             7950..8990
                     /gene="galS"
                     /locus_tag="ACHK0Z_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU4503440.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDSRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9270..10268
                     /gene="mglB"
                     /locus_tag="ACHK0Z_RS17395"
                     /old_locus_tag="pgaptmp_003479"
     CDS             9270..10268
                     /gene="mglB"
                     /locus_tag="ACHK0Z_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU4503441.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10329..11849
                     /gene="mglA"
                     /locus_tag="ACHK0Z_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             10329..11849
                     /gene="mglA"
                     /locus_tag="ACHK0Z_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU4503442.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11865..12875
                     /gene="mglC"
                     /locus_tag="ACHK0Z_RS17405"
                     /old_locus_tag="pgaptmp_003481"
     CDS             11865..12875
                     /gene="mglC"
                     /locus_tag="ACHK0Z_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU4503443.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12946..14181)
                     /gene="preA"
                     /locus_tag="ACHK0Z_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             complement(12946..14181)
                     /gene="preA"
                     /locus_tag="ACHK0Z_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU4503444.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14175..15413)
                     /gene="preT"
                     /locus_tag="ACHK0Z_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             complement(14175..15413)
                     /gene="preT"
                     /locus_tag="ACHK0Z_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU4503445.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLLLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         3739 a         3880 c         4121 g         3992 t
ORIGIN      
        1 atgcacatca ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc
       61 acccaggcgt cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgaccgac
      121 ctggaagggc agcttggcgt gcaactgttt gatcgcgtgg ggaaaagact ggttgttaat
      181 gaacacgggc ggctgctcta tccgcgtgcg ttggcattgc ttgaacaggc ggttgaaatc
      241 gaacaactgt ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt
      301 aactacattc tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt
      361 gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat
      421 attggtttta ttgaaggacc gtgccacagc actgaaatta tttctgaacc gtggctggaa
      481 gacgagctgg tggttttcgc cgcgccgact tcgccgttgg cccgtggtcc ggtcacctta
      541 gagcagctgg ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggagatt
      601 gtcgattatc tattgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac
      661 tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgg
      721 gtgattgaag atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc
      781 ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg
      841 cgctttctgg actattgcga tcccgcgaat gtaccgcgtt aagttgctgt acaagaacat
      901 gctggtgctg tgtcgatttc gtgacgcagc gccctcagca tgcattcgcc agaaaagaga
      961 ttggctgctt tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc
     1021 tgccggaagg attgccaatc gtctgctaca atcgcgcctc atttttaaga tggatagcat
     1081 ttttgtatgg tttccgaaac taaaaccaca gaagcgccgg gcttacgccg tgaattaaag
     1141 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
     1201 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
     1261 ggcctgatgg tttacttcct gatgaccagt ctcggtgaac tggctgcata tatgccggtt
     1321 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
     1381 ggctggaact actggtacaa ctgggcggtg actatcgccg ttgacctggt tgcagctcag
     1441 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
     1501 ggcgttatct tcctgctgaa ctacatctca gttcgtggct ttggtgaagc ggaatactgg
     1561 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
     1621 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
     1681 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
     1741 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
     1801 ccacgcgcgg tacgtcaggt gttctggcga atcctgttgt tctatgtgtt cgcgatcctg
     1861 attatcagcc tgattattcc gtacaccgat ccgagcctgc tgcgtaacga tgttaaagac
     1921 atcagcgtta gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
     1981 gtgatgaacg cagttattct gacggcggtg ctgtcagcgg gtaactccgg tatgtatgcg
     2041 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcacat tttcgctaaa
     2101 ctgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtctg
     2161 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
     2221 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
     2281 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
     2341 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
     2401 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
     2461 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgttac
     2521 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
     2581 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
     2641 ttaacatttg ggttgataat tgttatcgtt tgcattatcg ttacgccgca atcaaaaaag
     2701 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
     2761 accgcgatgc tgccgtacgc aaggggacgt gaagaagatg tgagcgataa cccattttat
     2821 tttcgtagtt acctcatgga gatatggaat gtttaggttg aaccctttcg tacgggtcgg
     2881 gctgtgtttg tccgctattt cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga
     2941 aacgatggtt gtcactgcat cttccgttga acaaaacctc aaagatgcac ccgccagtat
     3001 cagcgtcatt acccaggaag acctgcagcg aaaaccggta cagaatctga aggatgtcct
     3061 caaagaagtg cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat
     3121 tcgtggtctg gacagcagct acaccctgat tctcgtcgac ggtaaacgcg ttaactcccg
     3181 caatgccgtc ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga
     3241 acgtattgaa gtggttcgtg gcccgatgtc gtcgctatac ggctccgatg cgctcggcgg
     3301 tgtagtgaat atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga
     3361 taccaccatt caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag
     3421 tggaccatta attgatggtg tgctgggaat gaaagcttac ggcagcctgg caaaacgtga
     3481 aaaggatgac ccgcaaaact caacgaccac cgataccgga gaaacgccgc gtattgaagg
     3541 attctccagc cgcgacggca atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt
     3601 tactgccgga tacggtttcg accgtcagga tcgtgattcc gactcgctgg acaaaaaccg
     3661 cctggaacgc cagaactact ccgtcagcca taatgggcgt tgggattacg gcaccagcga
     3721 actgaaatac tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac
     3781 ttccgaaagc aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt
     3841 tctcacggtt ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg
     3901 gggaaccagc tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg
     3961 gatcttcgag ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg
     4021 tgaacactgg agtccgcgtg cctacctggt ttataacgcc accgacaccg taacggtgaa
     4081 agggggctgg gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac
     4141 gagcaattcc tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac
     4201 cagcgaaagt tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggt
     4261 tgaatccagc gttaccgttt tccgtaacga tgtgaaagat cgcatcagca tcagccgtac
     4321 gtctgacgtc aacgctgcac cgggctacca aaactttgtt ggttttgaga cgggcgctaa
     4381 cggacggcgc ataccggtat ttagctacta caacgttaac aaagctcgta ttcagggcgt
     4441 ggaaaccgaa ctgaaaattc cgttcaacga tgaatggaaa ctgtcgatca actacaccta
     4501 caacgatggt cgtgatgtca gcaacggtga aaacaaaccg ctatccgatc tgccgttcca
     4561 tactgctaac ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt
     4621 ttctggtcac tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg
     4681 ttataccatc tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc
     4741 aggcgtgctg aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga
     4801 cggacgtcgt tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac
     4861 cgggcggact ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa
     4921 gaggcgatga agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac
     4981 gctttctggc gcgcggcttc tgccagtacc gcaggttgca actggtcggc aagaaactga
     5041 tcattatcgc cctgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc
     5101 agcgcgcaac tgtcccattc cagccatgca tttttgtcct cacctaaata gcggctaaac
     5161 gctttgattc cccacgggac gctgcacgga ttcacaattg gcgcaaaggc cgaaacgctg
     5221 gtgtatttac ccggattttt cagcgccata atcagcgcac cgtgaccacc cattgagtga
     5281 ccgctaatgg cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca
     5341 tcgcgcagat aatcatacat ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga
     5401 taaaagcctg cgccctggcc taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc
     5461 gggctggtgt ctggcatcac cagtacaatc cccagttctg ccgccacgcg ctgggcaccc
     5521 gccttggtgg tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt
     5581 ggcggggtgt gatcacgagg gggtgggaga aagatactga acgtcatcgg gcagtttaag
     5641 gtactggagt cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc
     5701 atttccatgc gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca
     5761 atgtgctgtg agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat
     5821 gaaaggcagc ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc
     5881 acgcacaata atcaggctgt aaatcagctt aataactttg cccccacgca gggcggaggc
     5941 gtcacacctg caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca
     6001 tgaagcgtta gttgcgcgag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga
     6061 taacgaaacg cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa
     6121 tctcgacctg gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt
     6181 cgatgaaatt ttctccggtc tggattacgc caacttcccg aaaatcaccc tcattgaaaa
     6241 caaaatgaag gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga
     6301 acaccatttt gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt
     6361 gatcggtctg tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca
     6421 ggaacgtctg acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt
     6481 ggctgtctcg atcgacgcgg tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac
     6541 cagtgccaca acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca
     6601 cgagtttctg cgcgctgtgc gtcatcacaa ctgattaaaa ggcaggaacc atggagcgca
     6661 acgtcacgct cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca
     6721 gcgcctttgg gctaccaaag gcggcttatc tcaatcccgc ctggtacggt gctattacgc
     6781 cgcaggatgc atggacctgg gcatttcttg atctcatcgg ccaggtgaaa ttcctcacgc
     6841 tttttgcgct gctgtttggt gctggactgc aaatgttgct gccccgtggc agacgctgga
     6901 tccagtcgcg gttaacgctg ttagtcttat tgggctttat tcacggttta ttgttctggg
     6961 acggcgatat tctgctggct tacgggctgg tgggcttaat ctgctggcgg ctggtgcgcg
     7021 atgcgccatc ggtaaaaagc ctgtttaata ccggcgtcat gctttatctg gtggggcttg
     7081 gcgttttgct gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg
     7141 atgcatcggc tattttgtat gaaaaatact ggaagcttca cggcggcgtt gatgcgatca
     7201 gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact gggcgcacag tatggctggc
     7261 aactggctgg gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc
     7321 agttcagctt acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca
     7381 ttaaccttcc tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct
     7441 tcttacttca aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc
     7501 tgttttatgg cttctggccg caattaagcc gctttaagct ggtgctggca atcgcctgcg
     7561 tcggacggat ggcgctgacc aactatctat tgcaaacgct gatttgtacc acgctttttt
     7621 accacctcgg tttgtttatg cattttgacc gcctggagct gctggcgttt gttattccgg
     7681 tatggctggc gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg
     7741 tggaatggct ctggcgtcag ttaactttgc gtgctgccgg accggcaata tctaaaacat
     7801 caagataacg atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt
     7861 gactcgattc acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat
     7921 ttactgcaat ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg
     7981 ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg
     8041 acacgcgtga agcagtaatg aaagccgtga gtgagctgga ttatcggcca aacgccaatg
     8101 cccaggcgct ggcaactcag gttagcgaca ccattggcgt ggtggtgatg gacgtttctg
     8161 atgcgttttt cggtgcgctg gtaaaagcgg tggatctggt cgcccagcag catcagaaat
     8221 acgtgctaat cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt
     8281 taattcgcca gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac
     8341 tggcgcaatt tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt
     8401 acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt
     8461 tgctgaataa cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg
     8521 acgccatgcg taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg
     8581 aaagctggat tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac
     8641 tgctggggcg caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg
     8701 gcgcactgac agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg
     8761 gtttcgatga tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc
     8821 ccattgcttc aatgactaaa ttagccaccg aactggcctt gcagggggca gcaggcaata
     8881 ttgattctcg tgccagccac tgttttatgc cgacgttagt gcgtcgtcat tctgtcgcaa
     8941 cgcgccagaa tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa
     9001 tctgtgagtg atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaacg
     9061 ttgtaacccg tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac
     9121 aattaaagcc gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc
     9181 ttcggcgttc agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac
     9241 taccctgcat aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg
     9301 tgatggccag catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa
     9361 caatctataa gtacgacgat aactttatgt ctgtagtgcg caaagctatt gagcaagatg
     9421 cgaaagccgc gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc
     9481 agaacgatca gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg
     9541 ttgacccggc agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg
     9601 ttttcttcaa caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg
     9661 ttggcactga ttccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg
     9721 cggcgaatca gggttgggat ctgaacaaag acggtcagat tcagttcgta ctactgaaag
     9781 gtgagccggg ccatccggat gcagaagcac gtaccactta cgtgattaaa gagctgaacg
     9841 ataaaggcat caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg
     9901 cgaaagataa gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta
     9961 ttgctaacaa cgatgcgatg gcaatgggcg cggttgaagc gctgaaagca cacaacaagt
    10021 ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc tctggcgctg gtgaaatccg
    10081 gtgcactggc gggcaccgta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc
    10141 tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca
    10201 acaaagtggt ccgcgtacct tatgttggcg tagataaaga caacctggct gaattcagca
    10261 agaaataaga ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt
    10321 aaaattatat ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg
    10381 gtatcaacaa gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc
    10441 cacattctat ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat
    10501 gcctgtttgg tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg
    10561 atttccattc cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa
    10621 acctggtatt acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag
    10681 gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg
    10741 atattgatat cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga
    10801 tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt
    10861 cttcgttaac cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc
    10921 gcggctgcgg tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg
    10981 aagttaccgt attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga
    11041 tggacaagat catcgccatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag
    11101 aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt
    11161 cgattcgcga tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg
    11221 tgggggcgaa acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg
    11281 gcaccattac gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc
    11341 acggatttgc actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata
    11401 ttggttttaa ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg
    11461 ataactcgcg gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc
    11521 cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg
    11581 gtcgctggct actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg
    11641 atgtcggggc gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg
    11701 ggattattat tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg
    11761 tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa
    11821 ttctgcgtct tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata
    11881 agaaaagttt tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc
    11941 tggcgattat tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc
    12001 tcacccagtc atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc
    12061 aggggaccga tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat
    12121 tattgcagtc catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg
    12181 cgctggttat tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga
    12241 ttatcgctta tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct
    12301 atggcatcaa ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca
    12361 gtggcttctc tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca
    12421 tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct
    12481 tcggtaagaa catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca
    12541 acgtcggcct gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg
    12601 ggatgttaga agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc
    12661 tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg
    12721 tgattggcgt ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata
    12781 tcggcgttaa cccatactgg cagtacatca tcaaaggggc gattattatc ttcgccgtag
    12841 cgctggattc actgaaatac gcgcgtaaga agtgatgtga ttgtggatat tgccggatgc
    12901 gctgcatccg gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg
    12961 gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca
    13021 gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt
    13081 tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc
    13141 acagccaaca catttatcaa gattgatacg gggatagaca atataactgc ggtctaaatc
    13201 ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcagcg aatcaaatcc
    13261 ctgatcggcg agataatgcg acaacccgct tgccatatct tccactatcc gatacccgta
    13321 ctgcatgatg ccggtggtca cctgtaacgt tgctgcgccg agcaataaaa actcagccgc
    13381 atcctcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg
    13441 ggtgcgcatc tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc
    13501 ggaaatactc gattttccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt
    13561 ggtgatggat ttaacggtgt taatcgccgc aatgccatct gcgccgccgc gcttcgccgc
    13621 cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt
    13681 ggaaccccgt ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc
    13741 gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc
    13801 gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat
    13861 cgaagcgatc aatactttgt ccgggtaatc ttccttcagc cgacgcaggg cggccagatt
    13921 ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc
    13981 ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt
    14041 tttaaacacc acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt
    14101 gcctaccggc gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat
    14161 cgaaagatct ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg
    14221 gcttctttcc cggttttcac ggcatagacc acggttttgt caccctcaac aatatcgccc
    14281 gcagcaaaga cttgcggatc gcgggtctga taattttgtg tattgatagt gttacgctgc
    14341 ggctctaact ccgcaaaggc atccagtctg gcatgctgac cgacggcgag aataatttta
    14401 tctgccgcca tcgtcagttc gcccggtaac cgtacatgct taaacgtgac tttattgcct
    14461 tccacggcta ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggca
    14521 aactcttttt cgcttgccgg aaactcatct aactcttcac gcgctacgca ggttaccgcc
    14581 tgacagccga gaactttcag cgtgctggct acgtccatcg caacatcacc gccgccgata
    14641 attaatgcgc tttgtggaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaggaag
    14701 tccacggcaa tctcaacgtc gctatgctca aacagcaata acccggaacc gctcgataac
    14761 cccacggtga ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta
    14821 ccgacttcgt tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca
    14881 tccagcactg attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg
    14941 tgcgcttctt tctcataaat cgtgacgtca taaccctggt ttgtcagtgt tacactggcc
    15001 tgcaatccgg caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc
    15061 tgataaattt ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcggccg
    15121 atatcaatgg gagcatcaac accggcacgg gtacaaccgc tttgacataa tttctccgtc
    15181 gggcacactc tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg
    15241 cctttaaaat tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg
    15301 caagcctgac tacagggagc gtcatgacat aataaacagc gagaggcttc tttaatcgcc
    15361 agtaaaggcg taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct
    15421 tccttaatgc tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc
    15481 atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgtaagctc ctggggactc
    15541 actcacttgt cgccttcctg caactcgaat tatttagagt ataaatattg cgtgctgata
    15601 ttaaggctat gcgcgtgggg aaatttatac tgtgaaagga ttcacaaata gctgcggttc
    15661 agaatgttat atccgcagct tattgagtga agtaataatt attctcaata attaacagaa
    15721 aagagaaaat at
//
LOCUS       DAZFWP020000113        15694 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFWP020000113 DAZFWP020000000
VERSION     DAZFWP020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.113"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            278..682
                     /gene="sdhC"
                     /locus_tag="ACHK0Z_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             278..682
                     /gene="sdhC"
                     /locus_tag="ACHK0Z_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4503446.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            676..1023
                     /gene="sdhD"
                     /locus_tag="ACHK0Z_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             676..1023
                     /gene="sdhD"
                     /locus_tag="ACHK0Z_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU4503447.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1023..2789
                     /gene="sdhA"
                     /locus_tag="ACHK0Z_RS17430"
                     /old_locus_tag="pgaptmp_003486"
     CDS             1023..2789
                     /gene="sdhA"
                     /locus_tag="ACHK0Z_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU4503448.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2805..3521
                     /gene="sdhB"
                     /locus_tag="ACHK0Z_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             2805..3521
                     /gene="sdhB"
                     /locus_tag="ACHK0Z_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU4503449.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3722..6523
                     /gene="sucA"
                     /locus_tag="ACHK0Z_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             3722..6523
                     /gene="sucA"
                     /locus_tag="ACHK0Z_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU4503450.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6538..7755
                     /gene="odhB"
                     /locus_tag="ACHK0Z_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             6538..7755
                     /gene="odhB"
                     /locus_tag="ACHK0Z_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU4503451.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQIIGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7849..9015
                     /gene="sucC"
                     /locus_tag="ACHK0Z_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             7849..9015
                     /gene="sucC"
                     /locus_tag="ACHK0Z_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU4503452.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9015..9884
                     /gene="sucD"
                     /locus_tag="ACHK0Z_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             9015..9884
                     /gene="sucD"
                     /locus_tag="ACHK0Z_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU4503453.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(9988..10710)
                     /gene="mngR"
                     /locus_tag="ACHK0Z_RS17460"
                     /old_locus_tag="pgaptmp_003492"
     CDS             complement(9988..10710)
                     /gene="mngR"
                     /locus_tag="ACHK0Z_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGU4503454.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10879..12794
                     /gene="mngA"
                     /locus_tag="ACHK0Z_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /pseudo
     CDS             10879..12794
                     /gene="mngA"
                     /locus_tag="ACHK0Z_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            12812..15445
                     /gene="mngB"
                     /locus_tag="ACHK0Z_RS17470"
                     /old_locus_tag="pgaptmp_003494"
     CDS             12812..15445
                     /gene="mngB"
                     /locus_tag="ACHK0Z_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGU4503455.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            15470..>15694
                     /locus_tag="ACHK0Z_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             15470..>15694
                     /locus_tag="ACHK0Z_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503456.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
BASE COUNT         3759 a         3940 c         4320 g         3675 t
ORIGIN      
        1 tcaggataaa acccgacaaa ctatatgtag gttaattgta atgattttgt gaacggccta
       61 tactgccgcc aggtctccgg aacaccctgc aatcccgagc cacccagcgt tgtaacgtgt
      121 cgttttcgca tctggaagca gtgttttgca tgacgcgcag ttatagaaag gacgctgtct
      181 gacccgcaag cagaccggag gaaggaaatc ccgacgtctc caggtaacag aaagttaacc
      241 tctgtgcccg tagtccccag ggaataataa gaacagcatg tgggcgttat tcatgataag
      301 aaatgtgaaa aaacaaagac ctgttaatct ggacctacag accatccggt tccccatcac
      361 ggcgatagcg tccattctcc atcgcgtttc cggtgtgatc acctttattg cagtgggtat
      421 cctgctgtgg cttctgggta ccagcctctc ttcccctgaa ggtttcgagc aagcttccgc
      481 gattatgggc agcttcttcg tcaaatttat catgtggggc atccttaccg ctctggcgta
      541 tcacgtcgtc gtaggtattc gccacatgat gatggatttt ggctatctgg aagaaacatt
      601 cgaagcgggt aaacgctccg ccaaaatctc ctttgttatt actgtcgtgc tttcacttct
      661 cgcaggagtc ctcgtatggt aagcaacgcc tccgcattag gacgcaatgg cgtacatgat
      721 ttcatcctcg ttcgcgctac cgctatcgtc ctgacgctct acatcattta tatggtcggt
      781 tttttcgcta ccagtggcga gctgacatat gaagtctgga tcggtttctt cgcctctgcg
      841 ttcaccaaag tgttcaccct gctggcgctg ttttctatct tgatccatgc ctggatcggc
      901 atgtggcagg tgttgaccga ctacgttaaa ccgctggcct tgcgcctgat gctgcaactg
      961 gtgattgtcg ttgctctggt ggtttacgtg atttatggat tcgttgtggt gtggggtgtg
     1021 tgatgaaatt gccagtcaga gaatttgatg cagttgtgat tggtgccggt ggcgcaggta
     1081 tgcgcgcggc gctgcaaatt tcccagagcg gtcaaacctg tgcgctgctc tctaaagtct
     1141 tcccgacccg ttcccatacc gtttctgcgc aaggcggcat taccgttgcg ctgggtaata
     1201 cccatgaaga taactgggaa tggcatatgt acgacaccgt aaaaggttcg gactatatcg
     1261 gtgaccagga cgcgattgaa tatatgtgta aaaccgggcc ggaagcgatt ctggaactcg
     1321 aacacatggg cctgccgttc tcgcgtcttg atgatggtcg tatctatcaa cgtccgtttg
     1381 gcggtcagtc gaaaaacttc ggcggcgagc aggcggcacg cactgcggca gcagctgacc
     1441 gtaccggtca cgcactgttg catacccttt atcagcagaa cctgaaaaac cacaccacta
     1501 ttttctccga gtggtatgcg ctggatctgg tgaaaaacca ggatggcgcg gtggtgggtt
     1561 gtactgcact gtgcatcgaa actggtgaag tggtttattt caaagcccgc gctaccgtgc
     1621 tggcgactgg cggggcaggg cgtatttatc agtccaccac taacgcccac attaacactg
     1681 gcgacggcgt cggcatggct atccgcgcag gcgtaccggt gcaggatatg gaaatgtggc
     1741 agttccaccc gaccggtatt gccggtgcgg gcgtactggt caccgaaggt tgccgtggtg
     1801 aaggcggtta tctgctgaac aaacatggcg aacgctttat ggagcgttat gcgccgaacg
     1861 ccaaagacct ggcgggccgt gacgtggtgg cgcgttccat catgattgaa atccgtgaag
     1921 gtcgcggctg tgatggtccg tgggggccac atgcgaaact gaaactcgac cacctgggta
     1981 aagaagttct cgaatcccgt ctgccgggta ttctggaact ctcccgtacc ttcgctcacg
     2041 ttgatccggt gaaagagccg attccggtta tcccaacctg tcactacatg atgggtggta
     2101 ttccgaccaa agtgaccggt caggcgctga cggtgaacga gaaaggcgaa gatgtggttg
     2161 ttccggggct gtttgccgtt ggtgaaatcg cttgtgtatc ggtacacggc gctaaccgtc
     2221 tgggcggcaa ctcgctgctg gacctggtgg tctttggtcg cgcagcgggt ctgcatctgc
     2281 aagagtctat cgccgagcag ggcgcattgc gcgatgccag cgagtctgat gttgaagcat
     2341 ctctggatcg tctgaaccgc tggaacaata atcgtaacgg tgaagatccg gtggcgattc
     2401 gtaaagcgct gcaagaatgt atgcagcata acttctcggt cttccgtgaa ggtgatgcga
     2461 tggcgaaagg gcttgagcag ttgaaagtta tccgcgagcg tctgaaaaat gcccgtctgg
     2521 atgacacttc cagcgagttc aacacccagc gcgttgagtg cctggaactg gataacctga
     2581 tggaaacggc gtatgcaacg gctgtttctg ccaacttccg taccgaaagc cgtggcgcgc
     2641 atagccgctt cgacttcccg gatcgtgatg atgaaaactg gctgtgccac tccctgtatc
     2701 tgccagagtc ggaatccatg acgcgccgaa gcgtcaacat ggaaccgaaa ctgcgcccgg
     2761 cattcccgcc gaagattcgt acttactaat gcggagacag gaaaatgaga ctcgagtttt
     2821 caatttatcg ctataacccg gatgttgatg atgctccgcg tatgcaggat tacaccctgg
     2881 aagcggaaga aggtcgcgac atgatgttgc tggatgcgct tatccagctg aaagagaaag
     2941 atcccagcct gtcgttccgc cgctcctgcc gtgaaggtgt gtgcggttcc gacggtctga
     3001 acatgaacgg caagaatggt ctggcctgta ttaccccgat ttcggcactc aaccagccgg
     3061 gcaagaagat tgtgattcgc ccgctgccag gtttaccggt gatccgcgat ttggtggtag
     3121 acatgggaca attctatgcg caatatgaga aaattaagcc ttacctgttg aataatggac
     3181 aaaatccgcc agctcgcgag catttacaga tgccagagca gcgcgaaaaa ctcgacgggc
     3241 tgtatgaatg tattctctgc gcatgttgtt caacctcttg tccgtctttc tggtggaatc
     3301 ccgataagtt tatcggcccg gcaggcttgt tagcggcata tcgtttcctg atcgatagcc
     3361 gtgataccga gactgacagc cgcctcgacg gtttgagcga tgcattcagt gtattccgct
     3421 gtcacagcat catgaactgc gtcagtgtat gtccgaaggg gctgaacccg acgcgcgcca
     3481 tcggccatat caagtcgatg ttgttgcaac gtaatgcgta attaaatgtt gatgccgggg
     3541 aaatcccccg gctattcagg aaatctttaa aaactgcccc tgacactaag acagttttta
     3601 aaggttcctt cgcgagccac tacgtagaca agagctcgca agtgaacccc ggcacgcaca
     3661 taactgtgcg tggtagtatc cacggcgaag taagcataaa aaagatgctt aagggatcac
     3721 gatgcagaac agcgctttga aagcctggtt ggactcttct tacctctctg gcgcaaacca
     3781 gagctggata gaacagctct atgaagactt cttaaccgat cctgactcgg ttgacgctaa
     3841 ctggcgttcg acgttccagc agttacctgg tacgggagtc aaaccggatc aattccactc
     3901 tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct tcacgttact cttcaacgat
     3961 ctccgaccct gacaccaatg taaagcaggt taaagtcctg cagctcatta acgcataccg
     4021 cttccgtggt caccagcatg cgaatctcga tccgctggga ctgtggcagc aagataaagt
     4081 ggccgatctg gatccgtctt tccacgatct gaccgaagca gacttccagg agaccttcaa
     4141 cgtcggttca tttgccagcg gcaaagaaac catgaaactc ggcgagctgc tggaagccct
     4201 caagcaaacc tactgcggcc cgattggtgc cgagtatatg cacattacca gcaccgaaga
     4261 aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg actttcaata gcgaagagaa
     4321 aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt gaacgttacc tcggcgcaaa
     4381 attccctggc gcaaaacgct tctcgctgga aggcggtgac gcgttaatcc cgatgcttaa
     4441 agagatgatc cgccacgctg gcaacagcgg cacccgggaa gtggttctcg ggatggcgca
     4501 ccgtggtcgt ctgaacgtgt tggtgaacgt gctgggtaaa aaaccgcaag acttgttcga
     4561 cgagtttgcc ggtaaacata aagaacacct cggcacgggt gacgtgaaat accacatggg
     4621 cttctcgtct gacttccaga ccgatggcgg cctggtgcac ctggcgctgg cgtttaaccc
     4681 gtctcacctt gagattgtaa gcccggtcgt tatcggctct gttcgtgccc gtctggacag
     4741 acttgatgag ccgagcagca acaaagtgct gccaattacc atccacggtg acgccgcagt
     4801 gaccggacag ggcgtggttc aggaaaccct gaacatgtcg aaagcgcgtg gttatgaagt
     4861 tggcggtacg gtacgtatcg ttatcaacaa ccaggttggt ttcaccacct ctaatccgct
     4921 ggatgcccgt tctacgccgt actgtactga tatcggcaag atggttcagg caccgatttt
     4981 ccacgttaac gcggacgatc cggaagccgt tgcctttgtg acccgtctgg cgctcgattt
     5041 ccgtaacacc tttaaacgtg atgtcttcat cgacctggta tgctaccgcc gtcacggcca
     5101 caacgaagcc gacgagccga gcgcaaccca gccgctgatg tatcagaaaa tcaaaaaaca
     5161 tccgacaccg cgcaaaatct acgctgacaa gctggagcag gaaaaagtgg cgacgctgga
     5221 agatgccacc gagatggtta acctgtaccg cgatgcgctg gatgctggcg attgcgtggt
     5281 agcagagtgg cgtccgatga acatgcactc tttcacctgg tcgccgtacc tcaaccacga
     5341 atgggacgaa gagtacccga acaaagttga gatgaagcgc ctgcaggaac tggctaaacg
     5401 catcagcacg gtgccggaag cggttgaaat gcagtctcgc gttgccaaga tttatggcga
     5461 tcgccaggcg atggctgccg gtgagaaact gttcgactgg ggcggcgcgg aaaacctcgc
     5521 ttacgccacg ctggttgacg aaggcattcc ggttcgcctg tcgggtgaag actccggtcg
     5581 cggtaccttc ttccaccgcc acgcggtgat ccacaaccag tctaacggtt ccacttacac
     5641 gccgctgcaa cacatccata acggccaggg cgcgttccgt gtttgggact ccgtactgtc
     5701 tgaagaagca gtgctggcgt ttgaatacgg ttatgccacc gcagaaccac gcaccctgac
     5761 catctgggaa gcgcagttcg gtgacttcgc caacggtgcg caggtggtta tcgaccagtt
     5821 catctcctct ggcgagcaga aatggggccg gatgtgtggt ctggtgatgt tgctgccgca
     5881 cggttacgaa gggcaggggc cggagcactc ctccgcgcgt ctggaacgtt atctgcaact
     5941 ttgtgctgag caaaacatgc aggtttgcgt accgtctacc ccggcacagg tttaccacat
     6001 gctgcgtcgt caggcgctgc gcgggatgcg tcgtccgctg gtcgtgatgt cgccgaaatc
     6061 cctgctgcgt catccgctgg cggtttccag cctcgaagaa ctggcgaacg gcaccttcct
     6121 gccagccatc ggtgaaatcg acgagcttga tccgaagggc gtgaagcgcg tagtaatgtg
     6181 ttctggtaag gtttattacg acctgctgga acagcgtcgt aagaacaatc aacacgatgt
     6241 cgccattgtg cgtatcgagc aactctaccc gttcccgcat aaagcgatgc aggaagtgtt
     6301 gcagcagttt gctcacgtca aggattttgt ctggtgccag gaagagccgc tcaaccaggg
     6361 cgcatggtac tgcagccagc atcatttccg tgaagtgatt ccgtttgggg cttctctgcg
     6421 ttatgcaggc cgcccggcct ccgcctctcc ggcggtaggg tatatgtccg ttcaccagaa
     6481 acagcaacaa gatctggtta atgacgcgct gaacgtcgaa taaataaagg atacacaatg
     6541 agtagcgtag atattctggt ccctgacctg cctgaatccg tagccgatgc caccgtcgca
     6601 acctggcata aaaaacccgg cgacgcagtc gtacgtgatg aagtgctggt agaaatcgaa
     6661 actgacaaag tggtactgga agtaccggca tcagcagacg gcattctgga tgcggttctg
     6721 gaagatgaag gtacaacggt aacgtctcgt cagatcattg gtcgcctgcg tgaaggcaac
     6781 agcgccggta aagaaaccag cgccaaatct gaagagaaag cgtccactcc ggcgcaacgc
     6841 cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa gcccggcgat ccgtcgcctg
     6901 ctggctgaac acaatctcga cgccagcgcc attaaaggca ccggtgtggg tggtcgtctg
     6961 actcgtgaag atgtggaaaa acatctggcg aaagccccgg cgaaagagtc tgctccggca
     7021 gcggctgatc cggcggcgca accggctctg gctgcacgta gtgaaaaacg tgtcccgatg
     7081 actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag cgaaaaactc caccgccatg
     7141 ctgaccacgt tcaacgaagt caacatgaag ccgattatgg atctgcgtaa gcagtacggt
     7201 gaagcgtttg aaaaacgcca cggcatccgt ctgggcttta tgtccttcta cgtgaaagcg
     7261 gtggttgaag ccctgaaacg ttacccggaa gtgaacgcgt ctatcgacgg cgatgacgtg
     7321 gtttaccaca actatttcga cgtcagcatg gcggtttcta cgccgcgcgg cctggtgaca
     7381 ccggtactgc gtgatgtcga taccctcggc atggcagaca tcgagaagaa aatcaaagag
     7441 ctggcagtca aaggccgtga cggcaagctg accgttgaag atctgaccgg tggtaacttc
     7501 accatcacca acggtggtgt gttcggttcc ctgatgtcta cgccgatcat caacccgccg
     7561 cagagcgcaa ttctgggtat gcacgctatc aaagatcgtc cgatggcggt gaatggtcag
     7621 gttgagatcc tgccgatgat gtacctggcg ctgtcctacg atcaccgtct gatcgatggt
     7681 cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc tggaagatcc gacgcgtctg
     7741 ctgctggacg tgtagtagtt taagcttcac ctgcactgta gaccgacgtg gatagggtgt
     7801 cggtctacgg tttaaaagat aacgattact gaaggatgga cagaacacat gaacttacat
     7861 gaatatcagg caaaacaact ttttgcccgc tatggcttac cagcaccggt gggttatgcc
     7921 tgtactactc cgcgcgaagc agaagaagcc gcttcaaaaa tcggtgccgg cccgtgggta
     7981 gtgaaatgtc aggttcacgc tggtggccgc ggtaaagcgg gcggtgtgaa agttgtaaac
     8041 agcaaagaag acatccgtgc ttttgcagaa aactggctcg gcaagcgtct ggtaacgtat
     8101 caaacagatg ccaatggcca accggttaac cagattctgg ttgaagcagc gaccgatatc
     8161 gctaaagagc tgtatctcgg tgccgttgta gaccgtagtt cccgtcgcgt ggtctttatg
     8221 gcctccaccg aaggcggcgt ggaaatcgaa aaagtggcgg aagaaactcc gcacctgatc
     8281 cataaagttg cgcttgatcc gctgacaggc ccgatgccgt atcagggacg cgagctggcg
     8341 ttcaaactgg gtctggaagg taaactggtt cagcagttca ccaaaatctt catgggcctg
     8401 gcgaccattt tcctggagcg cgacctggcg ttgatcgaaa tcaacccgct ggtcatcacc
     8461 aaacagggcg atctgatttg cctcgacggc aaactgggcg ctgacggcaa cgcactgttc
     8521 cgccagcctg atctgcgcga aatgcgtgac cagtcgcagg aagatccgcg tgaagcacag
     8581 gctgcacagt gggaactgaa ctacgttgcg ctggacggta acatcggttg tatggttaac
     8641 ggcgcaggtc tggcgatggg tacgatggac atcgttaaac tgcacggcgg cgaaccggct
     8701 aacttccttg acgttggcgg cggcgcaacc aaagaacgtg taaccgaagc gttcaaaatc
     8761 atcctctctg acgacaaagt gaaagccgtt ctggttaaca tcttcggcgg tatcgttcgt
     8821 tgcgacctga tcgctgacgg tatcatcggc gcggtagcag aagtgggtgt taacgtaccg
     8881 gtcgtggtac gtctggaagg taacaacgcc gaactcggcg cgaagaaact ggctgacagc
     8941 ggcctgaata ttattgcagc aaaaggtctg acggatgcag ctcagcaggt tgttgccgca
     9001 gtggagggga aataatgtcc attttaattg ataaaaacac caaggttatc tgccagggct
     9061 ttactggtag ccaggggact ttccactcag aacaggccat tgcatacggc actaaaatgg
     9121 ttggcggcgt aaccccaggt aaaggcggta ctacccacct cggcctgccg gtgttcaaca
     9181 ccgtgcgtga agctgttgct gctactggcg ctaccgcttc tgttatctac gtaccagcac
     9241 cgttctgcaa agactccatt ctggaagcta ttgatgcagg catcaaactg attatcacca
     9301 tcactgaagg catcccgacg ctggatatgc tgactgtgaa agtgaaactg gacgaagccg
     9361 gcgttcgtat gatcggcccg aactgcccgg gcgttatcac tccgggtgaa tgcaaaatcg
     9421 gtatccagcc gggtcacatt cacaaaccgg gtaaagtggg tatcgtttcc cgttccggta
     9481 cactgaccta tgaagcggtt aaacagacca cggattacgg tttcggtcag tcgacctgtg
     9541 ttggtatcgg cggtgacccg atccctggct ctaactttat cgacattctc gaaatgttcg
     9601 aaaaagatcc gcagaccgaa gcgatcgtaa tgatcggtga gatcggcggt agcgctgaag
     9661 aagaagcggc agcgtacatc aaagagcatg ttaccaagcc agttgtgggt tacatcgctg
     9721 gtgtgactgc gccgaaaggc aaacgtatgg gccacgcggg tgccatcatt gccggtggga
     9781 aagggactgc ggatgagaaa ttcgctgctc tggaagccgc aggcgtgaaa accgttcgca
     9841 gcctggcgga tatcggtgaa gcactgaaaa ctgttctgaa ataaatatct gtaataagaa
     9901 atagccctcg ccgcttccct ctacaggaat ggcgaagggc tgtcggtttc gacatggttg
     9961 gccatcgtat gatggccttt tttgtgctta tcgcgatgat tttcgttgcg ctatcagggt
    10021 aaatttatag tcatcggtat taaaagcgtt gcggctatat tcaaacaccc gaccatcaac
    10081 taaatatcca cgcgatactt tttcaagaat cggctttgtc tggctgatat taagcagacg
    10141 gctcatctct tcggttggca tcagaggaat gatttcctgt tcgctacgat cgataaccat
    10201 tttcttcact tcttcaataa agtgatattt tgaattttcc atgacctgcc aggtgagatc
    10261 cgggaacaac gcaagcggca tccaggtttc ttccagcgcc attggctttt gcttgcgata
    10321 gcgcacgcgc ttcacatgcc acacacgatc ctgcgcggtg atttgtagct gttgctgaag
    10381 aaaatcgtca gccggaatca cttcaaatat caggacttca ctgtgtgtat cgacgtgacg
    10441 gtccgacagt ttttcatcaa aactggttaa ctgaaaaata tcgtaattga cccgctcttc
    10501 tttgacgtaa gtcccgctgc cctgaatgct ttcgaggatc tgttgctcga ctaactggcg
    10561 caaagcctga cgcaccgtaa cccggctgac gccaaactct gtttgtaatg ccgattcggt
    10621 aggtaacgca tcgccaggtt taagctcgcc acgcgcaatt tgttcacgaa tgcgatcggc
    10681 aatctgccgg tataagggct tgtgtcccat ttttagtatc tcattaatac gaatttagcc
    10741 attatgcccg ataaattcat cctgtaaata atacaaatac aatacaaata atttcaatca
    10801 agtgaaattg atcacataat ggtattgttt tatcgggcac actggcgcga ctataaaaac
    10861 gatcaagtga ggatcatgat gaatctgacg actctgaccc accgcgatgc gttgtgtctg
    10921 aatgcgcgct ttaccagccg tgaagaggcc atccacgcgt tgactcaacg tcttgctgct
    10981 ctggggaaaa tttccagtac tgagcaattt ctggaagaag tgtatcgccg tgaaagcctt
    11041 ggcccgaccg ccttaggtga agggttggct gtgccgcatg gcaaaactgc tgcggtaaaa
    11101 gaagcggcgt ttgcggtcgc cacactcagc gagccgcttc agtgggaagg cgttgatggc
    11161 ccggaagcgg ttgatttagt ggtgctgctg gcgattcccc ccaatgaagc gggtactacg
    11221 catatgcaac tgctgacagc gctgaccacg cgcctggcgg atgatgagat tcgggcgcgt
    11281 atacagtcgg cgacgacgcc tgatgagttg ctctcggcgc tggatgacaa gggaggcacg
    11341 caaccttctg cctctttttc caacgcgcca actatcgtct gcgtaacggc ctgtccggcg
    11401 ggtattgctc acacctatat ggctgcggaa tatctggaaa aagccggacg caaactcggc
    11461 gtaaatgttt acgttgaaaa acaaggcgct aacggcattg aagggcgttt aacggcggat
    11521 caactcaata gtgcaaccgc ctgtattttt gcggctgaag tcgccatcaa ggagagtgag
    11581 cgttttaatg gcatcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
    11641 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
    11701 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
    11761 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
    11821 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
    11881 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
    11941 cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
    12001 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
    12061 tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggattc ctgacttttt
    12121 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
    12181 aacctgtcgc ctggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
    12241 acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc ctttgacctt ggagggccag
    12301 tgaacaaagc cgcttatgca ttctgcctgg gcgcaatggc gaacggcgtt tacggcccgt
    12361 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
    12421 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
    12481 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
    12541 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
    12601 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
    12661 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
    12721 ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
    12781 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
    12841 cccgcatatg cattgggatc gggagtggta tttcaccacc gaagagtcac gcattctgct
    12901 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
    12961 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
    13021 agaccgcgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
    13081 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
    13141 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt cctttggcat
    13201 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
    13261 cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
    13321 tagcgaagtg acggcgcagg tgctgccgct gggctacgcc atcggtaagt acttacctgc
    13381 cgacgaaaac ggattacgta aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
    13441 tgtaaccaaa gagattttgc tgccgaatgg gcatgaccag atgccattgc agcaaaatat
    13501 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
    13561 ctttgaagag gtatttgaga agatcgaagc gcagcgtgaa agtctggcca ccctgaaagg
    13621 tgaatttatc gatggcaaat atatgcgcgt acatcgcacc atcggttcca cgcgcatgga
    13681 tatcaaaatt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
    13741 aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
    13801 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
    13861 tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
    13921 ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac tcgtgctgtt
    13981 taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcgcc tgcgcgccag
    14041 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
    14101 tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt acgatccctt
    14161 tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
    14221 tatcgaagcg aatcagcctg gcaacgtgat tgcagcaaaa agtgacgctg aagggatact
    14281 ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatgga acgctacaac tggtagataa
    14341 agatagcggt gtgcgctatg accgggtgtt gcaaattgaa gaaagctctg atgatggtga
    14401 tgaatatgac tattcacccg caaaagaaga gtgggtaatg acttctgcaa cagcgaaacc
    14461 gcaatgcgag attactcatg aggcctggca gagcagggct gttatccgct atgacatggc
    14521 ggtgccgctc aatttgttgg aacgaagcgt ccggcaatcc acgggcagag taggggtgga
    14581 gatggttgtc actctgagtc ataacagcag acgtattgat gtggatatca accttgataa
    14641 ccaggctgac gatcatcgcc ttcgcgtcct gatccctaca tcttttaaca ccgatagtgt
    14701 tctggcagat acccagtttg gttcgctaac gcgccccgtg aacgacagtg caatgaacaa
    14761 ctggcagcaa gaaggctgga aagaagcgcc ggttccggta tggaatatgc tcaactatgc
    14821 agccttacag gaagggcgta acggcatggc tgtctttagc gaagggctac gtgaatttga
    14881 agtcatcggt gaagagaaga aaacctttgc cattacgttg ctgcgtggcg tgggcttact
    14941 gggcaaagaa gatctgtttt taaggcctgg gcggccttcg ggaattaaaa tgccagtccc
    15001 ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt ttattgagtt ataccggtac
    15061 gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg ctgactccag tacagtgtta
    15121 caacaaaatc ccatgggatg cgatgaagct caacaaagcc ggattcaacg tgccggaaag
    15181 ttatagtttg ttgaaaatgc ccccagtggg atgcctgata agcgcactta agaaagctga
    15241 agaccgacaa gaagtgattt tacggctgtt taatccggct gaatcagcaa cctgtgatgc
    15301 gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa acgatgatgg atgaacacat
    15361 taccaccgag gaaaatcaag gttcaaatct atcggggcct tttttacccg gccagtcacg
    15421 gacgttcagt taccggcttg cctgaatagc aatcaaaccg aagccacata tgcgcggcca
    15481 gattgttgac aaagggcgct ttgttcatgc cggatacggc atgaacgctt tattcggtct
    15541 acaaaagcag gcaaattcaa tatattgcag agatgatgta gacactggca agcgtagcgc
    15601 atcaggtaat tttgcgttta tcttcactct caagccacgt atatgtggct ttatttttaa
    15661 caaaataata accctgggtg agttaattat aata
//
LOCUS       DAZFWP020000114        15513 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFWP020000114 DAZFWP020000000
VERSION     DAZFWP020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.114"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(106..774)
                     /gene="artM"
                     /locus_tag="ACHK0Z_RS17480"
                     /old_locus_tag="pgaptmp_003496"
     CDS             complement(106..774)
                     /gene="artM"
                     /locus_tag="ACHK0Z_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU4503457.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(774..1490)
                     /gene="artQ"
                     /locus_tag="ACHK0Z_RS17485"
                     /old_locus_tag="pgaptmp_003497"
     CDS             complement(774..1490)
                     /gene="artQ"
                     /locus_tag="ACHK0Z_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU4503458.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1497..2228)
                     /gene="artI"
                     /locus_tag="ACHK0Z_RS17490"
                     /old_locus_tag="pgaptmp_003498"
     CDS             complement(1497..2228)
                     /gene="artI"
                     /locus_tag="ACHK0Z_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU4503459.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2246..2974)
                     /gene="artP"
                     /locus_tag="ACHK0Z_RS17495"
                     /old_locus_tag="pgaptmp_003499"
     CDS             complement(2246..2974)
                     /gene="artP"
                     /locus_tag="ACHK0Z_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU4503460.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3192..3707)
                     /gene="ybjP"
                     /locus_tag="ACHK0Z_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             complement(3192..3707)
                     /gene="ybjP"
                     /locus_tag="ACHK0Z_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4503461.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3833..4156
                     /gene="ybjQ"
                     /locus_tag="ACHK0Z_RS17505"
                     /old_locus_tag="pgaptmp_003501"
     CDS             3833..4156
                     /gene="ybjQ"
                     /locus_tag="ACHK0Z_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU4503462.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4153..4983
                     /gene="amiD"
                     /locus_tag="ACHK0Z_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             4153..4983
                     /gene="amiD"
                     /locus_tag="ACHK0Z_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU4503463.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQIDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4980..5993)
                     /gene="ybjS"
                     /locus_tag="ACHK0Z_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     CDS             complement(4980..5993)
                     /gene="ybjS"
                     /locus_tag="ACHK0Z_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4503464.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6092..7522)
                     /gene="ybjT"
                     /locus_tag="ACHK0Z_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     CDS             complement(6092..7522)
                     /gene="ybjT"
                     /locus_tag="ACHK0Z_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU4503465.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7533..8534)
                     /gene="ltaE"
                     /locus_tag="ACHK0Z_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             complement(7533..8534)
                     /gene="ltaE"
                     /locus_tag="ACHK0Z_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU4503466.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8571..10289)
                     /gene="poxB"
                     /locus_tag="ACHK0Z_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             complement(8571..10289)
                     /gene="poxB"
                     /locus_tag="ACHK0Z_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU4503467.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10422..11390)
                     /gene="hcr"
                     /locus_tag="ACHK0Z_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             complement(10422..11390)
                     /gene="hcr"
                     /locus_tag="ACHK0Z_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU4503468.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11402..13054)
                     /gene="hcp"
                     /locus_tag="ACHK0Z_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /gene_synonym="priS"
     CDS             complement(11402..13054)
                     /gene="hcp"
                     /locus_tag="ACHK0Z_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU4503469.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13198..14097)
                     /gene="lysO"
                     /locus_tag="ACHK0Z_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             complement(13198..14097)
                     /gene="lysO"
                     /locus_tag="ACHK0Z_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU4503470.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14592..15287)
                     /gene="aqpZ"
                     /locus_tag="ACHK0Z_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             complement(14592..15287)
                     /gene="aqpZ"
                     /locus_tag="ACHK0Z_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU4503471.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         3819 a         4151 c         4066 g         3477 t
ORIGIN      
        1 aggcgtccac gccgcatccg gcacaggcac cgtgctgatg tctgatgcga cgctggcgcg
       61 tcttatcaga cctacaaacc cccccggcga atgtacgcag ctacattaat ttcgccgttc
      121 gaatgccagt gctttgcgct cgatcagacg catcatcagc gtcagcaggc cgttgacgac
      181 caggtaaata atccctgccg caccgaacac cattacatcg taggtgcgtc cgtacaacaa
      241 ctggctgtat cccatcactt ccatcagcgt aatggtgtaa gccagagagg tacttttgaa
      301 caccagcacc acttcgttgg aataagaaga gagcgagcgt ttaaaggcat acggcagcag
      361 gatcgccagc gtatcttttt tgctcattcc cagggcgcta caggactgcc actgaccttc
      421 tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta tacgccgcac tgttcagcga
      481 taacgcaatc agcgcacata accacggttc tgacaacaaa tgccacagtg ccggatactc
      541 ctgcaaagtc ggaaactggc ccggcccgta ataaatcagg aagatctgca ccagcagcgg
      601 cgtaccggta aacagcgtga tataaccccg caccagccac accagcaccg gcgttttcag
      661 cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc acaatcagcg aggcaacggt
      721 tagcgtcagg ctggtgtgta gccctttcat cagttcgggt aaatactcaa acattagctg
      781 ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt tgagaatgta ctgactgagc
      841 agggtgatca ccaggtaaat cgccgccgcc acaatgtacc aggtaaatgg ttcctgggta
      901 cgagtagcga tgctttttgt ttgcagcatt aaatcattca cactaatcaa actgaccagc
      961 gcggtatctt tcagcagcac cagccactgg ttaccgaggc caggcagcgc atgacgccac
     1021 atctgcggca tcaccagacg gaaaaagata gccgatttcg acagccccag cgcctgacca
     1081 gactcccact gacccaccgg caccgctttc aacgcgcccc gaagcgtttg cgaggcatag
     1141 gcggcataca gcagtgacag agcgatgaca ccacaaagga acgggctaac gtcgaagttc
     1201 tcaatgtcca tctgcactgg gatctgcacg aacccaagat tgatagtgaa gccatccgaa
     1261 agcgtcagca gcagctgcga ggagccaaaa tagataaaca gcaccaccag aatttctggc
     1321 aggccacgca gaatggttac cagcgctgaa cctgcccacg cgacaggacg ccattttgcc
     1381 gactcccata ccgcaaagaa catcgccagc gccagcccga caatcaatgc acaaacggca
     1441 aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa attcattcat caggaattac
     1501 ttctggaacc atttgttgta gatggtttcg taagtgccat ctttcttcac tttttccagc
     1561 gcagtgttga gtttctgctg cagctcagtg ttgccctgac gtaccgcgat gccgaggcca
     1621 gtgccgaagt aatctttatc ggtcactttg tcgcccaccg ccgccagttt cgggttatct
     1681 ttcagccact cagtgaccac tgcggtgtca ccgaagacgc cgtcgatacg cccgttttgc
     1741 agatccagtt ttgcgttctg gtagctgtca tacggaacgg tagtgatttc cgggtgctta
     1801 tccataatga atttctggtg tgtcgtcccg ttctgaacgc cgactttttt gcctttcagc
     1861 tgatcaacac tggtgtattt gccttgctga cccacaaaca gggcagagtt gtcatagtac
     1921 ggggtggtaa acagtacctg cttttcacgc tccggagtga tatccatgcc cgccatcact
     1981 gcttctacgc gacggaattt caggcttggg atcaggctgt caaacgcctg gttagagaaa
     2041 gtacaggttg catcaatctc tttacacagt gcctgtgcca ggtcgacgtc aaaaccaacg
     2101 atctggttgt ttgcatcaat cgattcaaac ggaggatagg aggcttcggt agcaaaacga
     2161 atggtttcgg cagctgtggc ggaaagacta aaacctgcaa ttaacgcggc aatcagaact
     2221 tttttcattg ttgttatccc gaatcttagt gagagagata gtttttaaat gcttcggttt
     2281 gcggctcggt aaagcagctc gcgtcgcctt gttctacgat atgaccattt tccatataca
     2341 ccactcggct ggcggtttta cgcgccactt caacttcgtg ggtgacgatc acctgggtaa
     2401 tattcgtttc tgccagctca cgaatgatgc tgacgatttg tgccgtaatt tccgggtcca
     2461 gtgcggcggt cggttcatcg aacagcagta cctgcggttc catcatcaac gcacgggcaa
     2521 tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg gtaacgatcg ctataaggtt
     2581 tgagacgcag acgttccagc agtttttctg cacgggccag cgcctgatct ttactcaacc
     2641 ccagtacacg gcagggcgct tcaatcaggt tttgctgcac ggtcagatgc ggccacaggt
     2701 tgtattgctg aaacaccatg ccaacgttac gacgcaaatc gcgaatcgct ttgtcagagg
     2761 gtgttttggt gaaatcgaaa tggttgcctg caatgttgag cgtaccggag cgcggcatct
     2821 caagcagatt gagtacacgc agcagcgagc ttttacccgc gccgctgggg ccaagtaaca
     2881 ccagcgtttc gccctgtggg caatccagcg tgatatcgaa cagcgcctga tgcgcgccgt
     2941 agaagcaatt aatgccgttt aattgaatac tcattgacac tcgtatactg gcagtctgat
     3001 agctattgag gtcgaagata gtacctttga cagaataatt atgcaatatt tctgctttaa
     3061 aagttaaaag caaagcgcat tattcaataa acatagcaca aaataacggg ggcggtggtc
     3121 ggtgagcata aatgtcggca ttcctcacga aatgccggac aatttacggg gtttattggt
     3181 tgatcaaggc gttagcgatt ctcgatggac tgacggagcg tacccgctgt ggcatgaacg
     3241 ctgccgccta aatagcgtac atcgtcgatc acccaacact ggccttcctg aatcattaac
     3301 acttcatcct gccagctctg atcaccctgt tttagatcca cacgcagagg aatatttcgc
     3361 gcatcacgat tcgggattgt tgaggcactg gcaacacggg cgctctctgg caaggttgtg
     3421 cgactggaga atggatcgtt ggtcagtagt tcccgatggt tattatcccg ggaggcatcg
     3481 ctaagcagtg tcgccagttt gtcgctcaga taagggcgca aggcggtgat gtcgttgctg
     3541 cggtgcaaaa tgcggtagtc ataaaattgc tgggccacgt tatccgggcc tccttcaacg
     3601 caaggaccac tgcgtgtgcc gttatcttta taagctggag tgactgtggt gcaggcactg
     3661 aggagcagtg cgcaggggat aagcattgtc aatttgctgt agcgcataat gatttcctta
     3721 taagcgatcg ctctgaaagc gttctacgat aataatgata tcctttcaat aatagcgtat
     3781 cagtctgata atgctttaga gatcgaaggc ttaacaaaca aggagatcga tcatgcaatt
     3841 ttctacaacc ccaactctgg aaggccagac catcgttgaa tattgcggtg tggtgaccgg
     3901 cgaagcgatt ttaggtgcca atattttccg tgatttcttt gccggtatcc gcgatatcgt
     3961 tggcggacgt tccggtgcgt atgaaaaaga actgcgtaaa gcacgggaga tcgcctttga
     4021 ggaattaggc tcccaggcgc gggcgctggg ggccgatgcc gtcgtcggta ttgatatcga
     4081 ctacgaaacg gtcgggcaaa acggcagtat gctgatggtt agcgtcagcg gtacggcggt
     4141 gaaaacgcgt cgatgagaag agtcttctgg ctgatcgctg ccgctctgtt actggcaggg
     4201 tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatatc agattgatac ccgacgccag
     4261 gcgcaggcgg cgtatccgcg cattaaagtg ctggtgatcc actacaccgc agatgatttt
     4321 gatagctcgc tggcgacact gaccgataag caggtcagct cgcattatct ggtccctgcg
     4381 gtaccaccgc gatacaacgg taaaccgcgc atctggcaac tggtgccgga acaagaactg
     4441 gcctggcatg cggggattag cgcctggcgc ggggcaacgc gccttaacga cacctctatt
     4501 ggcattgagc tggaaaaccg tggctggcaa aaatcggccg gagtgaaata ttttgccccg
     4561 tttgaaccgg cacagattca ggcgcttatt ccactggcga aagatattat tgcccgttat
     4621 cacatcaagc cggaaaacgt agtggcacat gcggatatcg caccgcagcg caaagacgat
     4681 ccggggccat tatttccctg gcagcaactg gcgcagcagg ggattggtgc ctggccggat
     4741 gcgcagcggg ttaactttta ccttgccggg cgcgcgccgc acacaccggt agataccgcg
     4801 tcattgctgg agcttttggc gcgctacggt tatgacgtta aacctgatat gacaccgcgc
     4861 gagcagcggc gcgtgattat ggcattccag atgcatttcc gcccgacgtt atataacggc
     4921 gaagcggatg cagaaactca ggcgattgcc gaagcattgc tggagaaata cgggcaggat
     4981 tagcgcggca gttttccgtg gtcgcgtagc caggcggcag ttttctcgat accttcatcc
     5041 agggtgatga ccggctgata acctaactct tcctgcgcac gcgtaatatc cagcgtaaag
     5101 tcaaaattca acttggagac gccgtagtgg gtcagcggcg gctcttttgc tgacttgcgg
     5161 cctaaacgct ccatgctgcg ggcgatcata tccagcatcg ggtaggggac ggaacgaata
     5221 cgacagtcaa tattcaactc gtcgatcagc ttctgcacga tgctgcgcag tgtgcgatgc
     5281 tcgccgttgg tgatgttgta cacacgcccg gaaggtagct tatcgcaggc ttcctggctt
     5341 gccagccaca ttgcgtgcac ggcattttca tagtaggtca tatcgaccag cgcactgccg
     5401 ccatgcggta acagaatact gccgtagtgg tgcatcatat gcgccagacg gggaataaag
     5461 actttatcgt gcggtccgaa cagactttgt gggcgcagaa tagtaaagcg cgtttgtgga
     5521 ttcgcctgcg aaagcatatt gatcacttct tcgctggctg ctttgctgcg ggcaaactcg
     5581 ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc gatggtggtg ataatcgaag
     5641 tacagggagg gggaagagat atgaataaag ttacgcacac cccaggcgac agcccattca
     5701 cccaggcggc gagtggcgcg aacgttagcc agatcgaaag cctgttgtgt cccccagggt
     5761 gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg cgagcatcac tttagcttgt
     5821 gatgaaacca gctcggtcag atccgccgga acaaactctg cgcccatttt ttccagcaat
     5881 ttgcccattg cctcgttgcg accggtcgct cgcacgctga tgcctttctg gcataaaaac
     5941 tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa ccagtacctt catatcaatc
     6001 cactgttgtt gagaaaataa cgtgcgcatt cttccgtgat ttcccccatg atgcaatggg
     6061 aaacatgaaa gaataacgca ggttttgtcg attaatctgt gctttgttct gccagtctgg
     6121 cgatttgttt tgccattccg cgaaaaataa acagatgcgc ggggatcatc aataaccagt
     6181 aaaacagccc cggcataccg tgcggatgcc agaaagcgcg gacatcgata gtacgatagt
     6241 cgcctttatc ttccaggcta aaacacagtc gtcccagccc cggcgctttc atgccaaata
     6301 acaacgtaag ttgtttttcc ggttcaacga caatcacttt ccagctatct accgcatcgc
     6361 cagtctgtaa atattcgcgc tccgggcggc ctttcgccag cttatgaccg atcgcgcggt
     6421 ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa ataacgctct ttaccgccga
     6481 tttggttcac tacctgccat aaagcagcaa ggctggcgga cgttttaacg gtaaaccccg
     6541 cctgtttggc aaaataaccg tactccggtc gccagcgggc aaaggcctga gcgtcgtagc
     6601 cccagtcgct ggagttgacc agtttttcct cctctttcaa cgtgctacgt accgcgtcat
     6661 cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc ggtatcatcc gccagcagat
     6721 cgtgtttcag cccctgaatc aacgccctgg cggtggtggg cggtacggaa gtaatcacat
     6781 tgagaaacca caccgaaatc cagcgggtgg ggagggggat ggggatcaac cagcggcgct
     6841 taccgctcac cgccataaaa tgttcaaact gttgctgata actgagcacc tctggtccgg
     6901 cggcttcgaa gatgcggtgt tcgctggccg gatgatctaa caacgccacc agatagtgca
     6961 gcaagttttc cagcgcgatg ggcgtggtgc gtgaacgtac ccagcgtggc ggcgttaaca
     7021 ctggcaggtt gtagaccata tcgcgcatga cttcgaacgc cgctgaacct gcgccaacga
     7081 taattccggc ccgaagttcg gtcacaggta cattcgcttc acgaagaatg tccgccgtag
     7141 cctgacgagc acgcagatga tccgactgct catgtggcgg ggcctgcaac gaactgagaa
     7201 agattaattg cttaactggt acttcacgta gcgcatcgcg gacgttgaga gccacctggc
     7261 gctcctgagc gataaaatcg ccgccttcgc ccatgctgtg caccagaaaa tagacggtat
     7321 cgatatcctg caacagggcc ggaaggttat ccggccagct gagatcgact ttatggcaac
     7381 tgacgtttgc cagttgcagc tttgcaagcc tgtcgacatg acgtgccgcc gccaggatct
     7441 gatgcccttg ctggctgagt gtgcgcacca gatgctgacc aatgtagcca ctggcaccga
     7501 gaactaaaat gcgttgcggc acgtctctct ccttaacgcg ccaggaatgc gcgccagtgg
     7561 gcggcgactt ccgccagttg ttcgcgcgag acgtcaagat gcgtcaccag gcggacaatc
     7621 ggcgaggcgt taatcagcac gtttctcgct ttcatgtatt cgcctaacgc ggcagcattt
     7681 tcttccccga cgcgaacaaa cagcatatta gtgtcctgac gcatcacatc cgcgcctgct
     7741 tcacgcagtt gctccgccat ccaggcggcg ttgtcgtgat cttcctgcaa ccgtgcgacg
     7801 ttatttttca gggcatacat cccggcggca gccagaatcc cggactgacg catcccgcca
     7861 ccggccattt tccgccagcg gatggcacgt ttaatgtaat cacgattacc gacgagtaat
     7921 gaaccgactg gcgtcccaag acctttcgac aggcaaatgg tgaacgaatc acaatattgc
     7981 gtgatctctt tcagttcgca gccgtaagcc accacggcat taaagatgcg cgcaccgtca
     8041 acatgcagcg ccagattgcg ctcgcgggta aattcccatg cttctttcag gtattcacgc
     8101 ggcagcactt tgccgttgtg ggtgttttcc agactgagta atttggtgcg ggcgaaatgg
     8161 atatcgtcgg gtttgatttt catcgccact ttatccagcg gtagcgtgcc gtcggcagcc
     8221 gcgtcgatgg gttgcggctg aatactgccc agcaccgctg cgccaccggc ttcaaacaga
     8281 tagttatgcg cggcctgacc gacaatatac tcttcgccac gttcgcagtg actgagcaga
     8341 gcgaccaggt tggcctgagt gccggtaggc agaaaaatgg cggcttcttt accggaaagc
     8401 tctgctgcat agtcctgcag agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca
     8461 accggggcgg ccatcatcgc ttcgagcatg gcgcggctcg gtcgggtaac ggtatcactg
     8521 cgtaaatcaa tcatggcatg tccttattat gacgggaaat gccacccttt ttaccttagc
     8581 cagttcgttt tcgccagttc gatcacttca tcgccgcgtc cgctgataat tgcgcgcaac
     8641 atatacaggc taaaaccttt ggcctgttcg agtttgatct gcggtggaat ggctaactct
     8701 tctttggcga ccaccacatc caccaacacc ggaccgtcga tagagaaggc gcgttgcagg
     8761 gcttcatcaa cttcagacgc tttttctaca cggatacccg taatgccgca cgcttcggca
     8821 atgcgggcaa agtttgtgtc gtgtagttcg gtgccgtcag tcaaatagcc accagctttc
     8881 atctccatcg ccacaaagcc cagcacgctg ttgttaaaga cgacaatttt cactggcagt
     8941 ttcatctgca ctactgagag gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac
     9001 atggcgacca cctgacgttc tggctctgtc gcctgcgcac ccagcgcctg cggcatggcg
     9061 ttagccatcg aaccgtggtt aaacgaacct aacaggcgac gcttgccgtt catttttaga
     9121 taacgtgccg cccacaccgt tggcgtacca acgtcacagg tgaaaatagc gtcatcggcg
     9181 gcaaaatgac taatttgctg cgccagatat tgcgggtgaa tggctttctc gctcggttta
     9241 gctaaatcgt ccagcccttt gcgggcgtcg cggtaatctt ccagcgcttt atccagaaac
     9301 ttgcgatcgg ctttttcttc caccaatgga agcaatgcac gcagagtcga cttgatatcg
     9361 ccgaccagtg ccatatccac cttgctgtga gcgccgatgc tggctgggtt gatatcaatc
     9421 tgaatgattt tggcatcggt cgggtagaag gcgcggtagg gaaattgcgt gccgagtagc
     9481 actaacgtgt cggcgttcat catggtatgg aaacctgacg agaagccgat taacccggtc
     9541 attccaacat catacggatt atcgtattcg acatgttctt taccgcgcag ggcatgaaca
     9601 ataggcgctt taattttccc ggcaaactca actaactctt tatgcgcccc cgcgcagccg
     9661 ctgccacaca tcagggcgat attgctggaa taacgcagca gttgcgccag tttgcgtaac
     9721 tcttcttctt ccggcgtcac gactggttgt ggcgcatgat accagtgcgt ggttgcccct
     9781 tctggcgcag gttttaacgc cacgtcgcct ggtaacacga caaccgaaac gccacggtta
     9841 agcaccgctt tgcgcatggc aatcgccagt acttgtggga tctgctccgg gctggaaacc
     9901 aactcgcaat agtgactaca ttcgcggaat agctcttgtg ggtgggtttc ctggaaatag
     9961 ccgctgccaa tttcgctgga gggaatatga gcggcaatcg ccagtaccgg aacgtgattg
    10021 cggtggcaat cgaacaggcc gttgattaag tgcaggttgc cggggccgca cgatccggcg
    10081 cagaccgcca gttctccgct aagttgtgct tcagcgccag cggcaaaggc cgccacttct
    10141 tcgtggcggg tggacatcca ctcgatggtg cccatgcgat taagactgtc actaagaccg
    10201 ttcagagagt cgccagtgac tccccagatg cgtttcaccc ctgccgattc gagtgttttg
    10261 gcgatataag ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt gataacggta
    10321 acaagtttag ttcatctgac ggagggggaa gggatgggag agaaaggagg cactaacggt
    10381 taaatagccc gatgaaagga atatcatcgg gcataaggcg attatgcgag aaccaaatcc
    10441 ccctgcggat ggcaggagca ggccagtacg taaccttcag cgatttcggc gtcggtcagc
    10501 gtcattgtac tgctcaccgt atattcaccg gaaaccactt ttgtcttaca gcagccgcaa
    10561 acacccgcac ggcaggcagc gacaaccgga acgttattgc tttccagcgc ctccagtagt
    10621 gtggtgccaa ccggggcgta aaattctcgt gccggttgca gtttggtgaa tttcagaccg
    10681 ctggtcgccg cttctgctac tggggtgaag aatttctctt taaagaaacg cgtcacgccg
    10741 agcgctttca cttcctgctc tacccaatcc atatacggag ccgggccgca ggtcatcacg
    10801 gtacgtgaag ctaagtcagg tacacctgcc agcagttcgc gagtgagacg accagcgata
    10861 aagccttcgg taacgttatt ttctgccacc agcgttaccg gatagttacg ccactcatcg
    10921 gcgaaaataa catcctgcgg cgtacgcacg ttgtagatca cccgcacatc ggcctgtgga
    10981 cggttcttcg caagccagcg acgcatcgac ataatcggcg tgacgccgca gcctgccgcc
    11041 agcaacagga atttatcttc tgctttatcg tcgcaggtaa attcccccat cgcgtccgaa
    11101 agccagagat aatcaccgcg ttttacatcg cgcgtcagcc actgggagcc gacaccgtca
    11161 tcaatccgcc gcacggtcag ggtgatatat tcactcacgc ctggcgtgga ggaaatggtg
    11221 taagcacgca gcgtttccgc tgagtttcgc acgctgacca gtgcatattg cccggcgcga
    11281 tatgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt ttcttgcgta
    11341 atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat cgacaaactc
    11401 cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa cgcaggccaa
    11461 atttctcgtt cagcaccgcc agcaggtctg gtgtcaggaa accaggtgca gtcgggccgg
    11521 tgacgatatt tttcacgccc agagaaagca gcgtcagcag aatgacgatc gctttctgtt
    11581 caaaccagga gagcaccagc gacagcggca gatcgttgac accgcagccc agtttctctg
    11641 ccagagtgac agccagaata atcgctgagt aagcatcgtt acattgacct gcatctacca
    11701 gacgcggcag accttcgata tcgccaaact caagtttgtt aaagcgatat ttaccacagg
    11761 cgagggtcag gatcaggcag tcatccggca cgctggtggc gaaatcggtg aagtagtggc
    11821 gctcgccgcg tgcgccgtca cagccaccaa gcaggaagat atgacgcagt ttttcacggc
    11881 tcaccagatc aatcagcgta tcagcagcgc caagcagcgt ctggcgacca aaacccacgg
    11941 tgatcaggtg cggaatttcg ctgtacggga agcccgccat ttgttgcgcc tgggcgatga
    12001 ccgcagaaaa atcttcgcca tccagatgac gcacacccgg ccagccaacg atgctgcggg
    12061 tccagatacg atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc gaggtcatca
    12121 cgatggggcc agggaaacga gcgaactcca cttgctgatt ctgccagcca ctgccgtagt
    12181 taccgaccag atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc agcatttcgc
    12241 cgtgggtgta gacattaacg cccgtgcctt cggtctgttc cagcaggttg tagagatctt
    12301 tgagatcgtg accggaaatc agaatgcatt tacccgccgt cgctttgacg ttgacctggg
    12361 ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcatc cagaatgctc atcactttga
    12421 agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg gcaggccagg
    12481 tccccagcca cgccatgatt ttatggtact gggcataaat atcgttgtcg tattgaccga
    12541 gaacatgcgc gtgttccata taggccgccg cacctttcag gccatacagg cacagcagac
    12601 gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta aattctgctg
    12661 cctgacgttg cagctcgccg agatcgtcgc tcaccagttg caggtcagcc atcgggttat
    12721 cgacgcgcgc gttggcatct acagccaggc attgcgcttt cagcgcctcg cgcagggcaa
    12781 tcgcttcacg agcgtagccg acaatacgcg gagaatcgaa gttaacgttg gtcagggttg
    12841 agaaaaaggc acgtggcgcg aagctgtcaa catcgtggtt gatgatgccg tattcacgcg
    12901 cttttaccgc ccaggcagaa agcccttgca gcgccgcgat gagtaaatcc tgaaggtcag
    12961 aagtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt cctgccggag
    13021 tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa gttatattta
    13081 atatacatgt ttaaggttaa gacgcttaac gcggggataa aagggatttt tcatgcaact
    13141 ttaagggaga ttgatttagc gcaattttgg cggcagggat ctaccgccag agaggtatta
    13201 cgcagagaaa aaggcgatga ggatcggcac taacaggcta agaataaaac cgtgaacaat
    13261 tgccgccggg accatatcca gcccgccagt acgttgaaga acgggcaggg tgaaatccat
    13321 tgatgtggca ccgcataagc ccagtgcagt agagcggctg cggcgaatca gcccagggat
    13381 caacataata gcaatcagtt cacgggccag atcattaaaa aacgccgcgc tcccgattac
    13441 cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga aaccggaggc
    13501 cattgccagc gcggtattga tggggagatc aagaataaag gcgttaatta aaccaccaat
    13561 taatgaactg acaaccacca ccacggcgac aatcattccc cggcgattaa ggacaatctg
    13621 ctttaaggtc atgccattat tgcgcaactg aataccaacg aggaaaagta gcaaaattaa
    13681 cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca gaccaatggc
    13741 aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg caatacgcga
    13801 cgggagtttt tcttgctgat ggtggttgcg ccacggcagg cctcgctcca gccacatcag
    13861 ggcggcaata ttacacagta aaataacggt aatactgacg gcagaataat gcagaatcgc
    13921 caacaggtta ctggcgaggt tatcgagaaa cgccagactg atacccataa aaaagagaat
    13981 aaggtaaacc atccagctta atagctgatt aataactttt aacgcagctt gttggcgaag
    14041 cggaatgagg taacccacaa tcaggggaac cagaatgatt aacagcccag aaaacatgaa
    14101 aacccagtcc ttgcaaagat gaagtcgaaa tgcgcgatga cacactactg aaagcggaag
    14161 gacgagtaaa gttgcaatta aaaggaaatg ttatgcataa ggagcagtag agtattcgtt
    14221 ttcatttaaa gatattcttg cgctttaatt acaaactgca ccgatgttgg tggcgtcaaa
    14281 atcgccgagg cgttccctga aggccggggc agcccacatg gatgtgggct gagggcgcgt
    14341 tttacaggga tgttacctcg cgcccgaccc ggtagccgta agggataagt cgagggcacc
    14401 gcgcagcggc gattttgttc gccagagccc gggggtgcag ggggaggagg cgattgtcct
    14461 ccccctgcgc gctccttgcg ccagtggcaa tatgtggctt agctcatgaa aggagcgcaa
    14521 caagatgatg aatcaacata taacaacatc ttaaaaaaag gcctgacatt acgccaggcc
    14581 ttctgcgtta attaatcacg cttttccagc agggtccggt aaatcagacc accgataatg
    14641 ccgccgacaa ttggcaccac ccagaagaac cacagttgtt ctaatgccca gccgccctgg
    14701 aagatagcaa ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac cggaatacta
    14761 attaagtgaa tcagggttaa ggccagacca atagcgatcg gcgcaaaacc tgccggcgcg
    14821 aatttgtcgg ttgcgccgtg gatcaccaac aggaaacctg cactcaatac cagttcaact
    14881 accagcgcgg aaagcatgga ataaccgcct ggtgaatgct cgccataacc gttagaagca
    14941 aaaccgctgg ctgccgcgtc aaaacccgtt ttaccactgg caattaaata cagcagcgcc
    15001 gctgcaacaa taccgccgac aacctgggca attacgtagc caacgacttc ttttgccgga
    15061 aaacgtccgc cagcccataa accaatagtg accgccgggt taaaatgacc accagaaata
    15121 tgaccaacag caaaggccat cgtcagaacg gtcagaccga acgccaacgc cacgccggca
    15181 aaaccaatgc ctaattccgg gaagcctgcg gccagtacag cactaccaca gccaccaaaa
    15241 acaagccaga aagtaccaaa acattcagct gctaattttc tgaacatatc cacctcaatt
    15301 aaaaattgac cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa aagtgacgac
    15361 ggaaataatg cgcggctatt ttaaaaacga aggcgagtca ttcaccagat aaataaatcc
    15421 agtaaatttg atttagggca acagcgggtt gccccatata gtcatttgtc tgattgacag
    15481 tgtagtgcaa gcaaaagatt taatccttta ggc
//
LOCUS       DAZFWP020000115        15392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFWP020000115 DAZFWP020000000
VERSION     DAZFWP020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.115"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..294)
                     /locus_tag="ACHK0Z_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /pseudo
     CDS             complement(1..294)
                     /locus_tag="ACHK0Z_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(593..757)
                     /locus_tag="ACHK0Z_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /pseudo
     CDS             complement(593..757)
                     /locus_tag="ACHK0Z_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(883..1284)
                     /gene="eptO"
                     /locus_tag="ACHK0Z_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /gene_synonym="gspS"
     CDS             complement(883..1284)
                     /gene="eptO"
                     /locus_tag="ACHK0Z_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HGU4503472.1"
                     /translation="MRINTLKKLNCITSLLVLVTVAGCHQVPSTRKQATVPPSEQLEQ
                     TASIAAATRYLKRRCNRSDLPDDQAILNGVNRIANGKGWQSLTQEDIRKHSDEINERL
                     ARDSTPEHIKCSEFNRLLVPFIGELLAGTSR"
     gene            complement(1378..2136)
                     /locus_tag="ACHK0Z_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             complement(1378..2136)
                     /locus_tag="ACHK0Z_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4503473.1"
                     /translation="MGSFLNVVIHRLPVILERRWRREALMLLAHSEPESEPVFNLMIP
                     RSHCPYCFQPLSIRDNIPLLSYLFLKGKSRCCGERIPIYYPLVEITSSVLFILAASRF
                     HPGLTLVAAWLFIAMLLVLAVIDCRTAMLPDVLTLSLLWLGLLFSLQGGRVPLEEAVV
                     GAVSGYLCLWGLYWLFRFVTGREGLGYGDFKLAAALGAWMGWQVLPSVLLFASVSGLI
                     ITVLLRFVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            complement(2263..2778)
                     /gene="gspM"
                     /locus_tag="ACHK0Z_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             complement(2263..2778)
                     /gene="gspM"
                     /locus_tag="ACHK0Z_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HGU4503474.1"
                     /translation="MNDLKKRLQYISPRERRLLTGCVALLVVAFVYYVFWLPWQVRED
                     KWRTVIAREKYTVEWMKQQIPSIKKWEASMPEQGEPLGLSAAVTQTSAAYGVSVTRLQ
                     PQGERLTVTLAPVEFNTLMQWLTQLHRRYRIRTVVFDVVAQGNQPGHVTVNRLVLSRD
                     DINRDTSPGHL"
     gene            complement(2765..4042)
                     /gene="gspL"
                     /locus_tag="ACHK0Z_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             complement(2765..4042)
                     /gene="gspL"
                     /locus_tag="ACHK0Z_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGU4503475.1"
                     /translation="MERGAFPEHSATVWINGEGGTLNKNNKHVEAGRLFLRLPEDDTQ
                     PLLWRLVSPEETGGGSVAVGETDPLLAGLLPRYPAWVLVPSDRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQTGGRCNVAVVEKSVMHHWLTYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVRLAGQWLFRSDRYAGMTVELSWLTQFLSLTAPPVIESYSV
                     PPAQCMGLESTEWRTQPVRDLLQLAAECEGYDGADLRQGIFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAEFWQHESVRVYQELFPGETVVKDPHRQMLRHLQ
                     QSTSNNMPELGGVMHQLQQLLPETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     SAGEKYIIQPGEIRQQPHGVESRLILRMNNERS"
     gene            complement(3976..4953)
                     /gene="gspK"
                     /locus_tag="ACHK0Z_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     CDS             complement(3976..4953)
                     /gene="gspK"
                     /locus_tag="ACHK0Z_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGU4503476.1"
                     /translation="MKSREQGVALLVVLLILSLMVTIAAAIAERNGRTFWRTVAQLDQ
                     LQAKWDGYAAEEMAIQILQRSRQASPRKTHLAQNWAQPELQFETGGGDVRGRIVDAQA
                     CFNLNAINYGAVDLAGVPYAARIFQQLLINLQVDIIQARQVTAALRDWIDRDDEPVKE
                     GAEDEVYMGMEPPYLAASQPMQDVSELRLIRGIDVRLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPNSLLVTKLLLRRPRTGWDSVAAFLDSPQLKGIDTSTAISV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRSIQRQYGLTVREVP"
     gene            complement(4950..5549)
                     /gene="gspJ"
                     /locus_tag="ACHK0Z_RS17590"
                     /old_locus_tag="pgaptmp_003518"
     CDS             complement(4950..5549)
                     /gene="gspJ"
                     /locus_tag="ACHK0Z_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGU4503477.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQSGIRAHELSQD
                     KVRRLAELQRGGSQIERDLMQMIPRHSRGSEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFYVDHPSGIAPDVRVVLEGVHAFRLRFFVNGT
                     WQARWDSTSILPQAVEVTLVMDDFAELTRLFLVSKETAE"
     gene            complement(5546..5911)
                     /gene="gspI"
                     /locus_tag="ACHK0Z_RS17595"
                     /old_locus_tag="pgaptmp_003519"
     CDS             complement(5546..5911)
                     /gene="gspI"
                     /locus_tag="ACHK0Z_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGU4503478.1"
                     /translation="MKRQEGMTLLEVMVALVVFALAGIALIQSTTQQAAGIGPMEEKV
                     LAGWLADNQMVQLQLEKIWPKNGWDEKTISFAGREWYLRWHGRDSDIPEQRILEVEVR
                     RTREETSALVSLRGSVVRE"
     gene            complement(5908..6459)
                     /gene="gspH"
                     /locus_tag="ACHK0Z_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             complement(5908..6459)
                     /gene="gspH"
                     /locus_tag="ACHK0Z_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGU4503479.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSMQDGLLIGIQVRSDGWTFKVLQRVTAESFPASVGGDVWQGYVWQTWQ
                     PRRAAMEGKLPDDVRLDLQYLRGLQWMPEHNDGTGPDILLLPGGEVTPFSLLFRLVGG
                     EAVTGLQVDENGLMTLFEGEVEL"
     gene            complement(6456..6890)
                     /gene="gspG"
                     /locus_tag="ACHK0Z_RS17605"
                     /old_locus_tag="pgaptmp_003521"
     CDS             complement(6456..6890)
                     /gene="gspG"
                     /locus_tag="ACHK0Z_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGU4503480.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     VSDLVALESALDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSNIDIFSPGPDGIPNTEDDIGNWTPGHTQP"
     gene            complement(6921..8144)
                     /gene="gspF"
                     /locus_tag="ACHK0Z_RS17610"
                     /old_locus_tag="pgaptmp_003522"
     CDS             complement(6921..8144)
                     /gene="gspF"
                     /locus_tag="ACHK0Z_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGU4503481.1"
                     /translation="MALFHYQASDIHGRKRRGVLEADSARHARQLLREQALIPVRLEE
                     KQLHHQHSPRSILRFRPRGVSSAELALLTRQLSTLVAASLPLEEALDALSRQSEKPRQ
                     RDLIAAVRARVLEGHSLATAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALIYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGL
                     TEVCQTWGPWLLLAAATGWIAGRMMLHKPSRRLAFHHLLLRLPIVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMYISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPS
                     MMRHMIASGENSGELDSMLERAADNQDREFSAQMQLALGLFEPMLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNI"
     gene            complement(8146..9648)
                     /gene="gspE"
                     /locus_tag="ACHK0Z_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             complement(8146..9648)
                     /gene="gspE"
                     /locus_tag="ACHK0Z_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGU4503482.1"
                     /translation="MSSIQNVSESRPLLPFSFSRTQRILLLREQEGNQVFCMEDTPAS
                     ALLEVRRVAEGPLNVTTLSAEAFEKQLVSCYQRDSDEARQMMADIGNEMDFYTVAGEL
                     PDSEDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRVDGVLREILR
                     LRRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIVL
                     RLLDKNSVSLDLAALGMSPQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSRLN
                     PQDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDSET
                     AQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLCP
                     RCRQSCQASAELAKDMDIPPETTIWQPAGCQHCSFTGYHGRTGLHELLLIDDRIRMAI
                     YQGEGELSITRLAGGRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            complement(9645..11615)
                     /gene="etpD"
                     /locus_tag="ACHK0Z_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             complement(9645..11615)
                     /gene="etpD"
                     /locus_tag="ACHK0Z_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HGU4503483.1"
                     /translation="MFRKWLNGRLSIFVFTTVFLSAIPGWGAEFSASFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDASPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVVKRLMEIVERVDKEGNRSVATVPLTYASATDVARLVTELTKES
                     DKTAMPAWMTAKLVADERTNSVLVSGEPISQQRIISMIKQLDRKEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSNIENDAKKSSSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEVQDADGLNLGIQWVNKHSGVTQFTSTGLPITTMVQTR
                     QSDISDSDQSNTLSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFSSKSEHAAKRNLMLFIRPTIIRERDGFRHASAEKYLSFNQEQVQSRGKETMALTL
                     NEEQLKLSRDRDDTTFRKVKTAIAAFYARGMQ"
     gene            complement(11616..12491)
                     /gene="gspC"
                     /locus_tag="ACHK0Z_RS17625"
                     /old_locus_tag="pgaptmp_003525"
     CDS             complement(11616..12491)
                     /gene="gspC"
                     /locus_tag="ACHK0Z_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGU4503484.1"
                     /translation="MLFFLSSRRDRNLFIKDIALKMLTPNWVLCVILLIAGYQLVSVI
                     RHFWLTPATSASDLSHVSVSETAVTDEHTEENFVFTLFGTASPPLSEGKVQKTTSSLS
                     DDLLSGGDLDVRGILYSSVTEHSVAIFAHNNRQFSLGIGEKVPGYDATISAIFSDHIV
                     INYQGKNASLPLRYDNPAKRNAQDDNNLIVGPVTTQANFRVKNIFDIMSLSPVTVNNT
                     LSGYRLSPGKASSLFYNAGLHDNDLAVLLNGSELRDTRQAKQIMKQLTELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            complement(12575..15235)
                     /gene="stcE"
                     /locus_tag="ACHK0Z_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     CDS             complement(12575..15235)
                     /gene="stcE"
                     /locus_tag="ACHK0Z_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HGU4503485.1"
                     /translation="MKLKYLSCMILASLAMGAFAATAADNNSAIYFNTTQPVNDLQGG
                     LAAEVKFAQSQILSAHPKEGESQQHLTSLRKSLLLVRLVKADDKTPVQVEARDANDKI
                     LGTLTLSPPSSLPDTVYHLDGVPADGIDFTPQNGTKKIINTVAEVNKLSDASGSSIKS
                     YLANNALVEIQTANGRWIRDMYLPQGAELEGKMVRFVSYAGYNSTVFYGDRKVTLSVG
                     NTLLFKYVNGQWFRSGELENNRIAYAQHTWSAELPAHWIVPGLNLVIKQGNLSGSLND
                     INVGAPGELLLHTIDIGMLTTPRGRFDFAKDKEAHREYFQTIPVSRMIVNNYAPLHLK
                     EVMLPTGTLLTDADPGEGGWHSGTMRQSIGKELISHGIDNANYGINSTAGSGESSHPY
                     VAAQLTAHTNRGNYSNGVQVHGGSGGGGIVTLDSTLGNEFSHEVGHNFGLGHYVGGFR
                     GSVHRSADQINSTWGWDSDKKRFIPNFYPAQTNRKSCLDEQCQEPFDGHSFGFDAMAG
                     GSPFSDANRFTMYTPNSSAIIQRFFENKAVFDSLSSTGFSKWNADEHKMEPYEHTIDR
                     FEQITASVRDLSENKMAEMMAEYPIVKVHMSDGNWTRDIHAPAASAENKGRILTLDHE
                     AGYNSHLFINGGEKIISKGYKKSFVSDGQTWKESDVVNTREARKPEKFGVPVTTLVGY
                     YDPKGTLSSFIYPAMYGAYGFTYSDDSENLSSNDCQLQVDTREGQLRFKLANNRVNSS
                     VMNKFHINVPTESQPTQAILVCNNKVLDTRTLTPAPEGLTYTVNGRALPAKENEGCIV
                     SVKTAQRYCLPVGQRSGSSLPDWIAGQEVYVDSGAKAKVLLSDWDNLSYNRIGEFVGN
                     VNPADMKKVKAWNGQYLDFSRPRSMRVVNK"
BASE COUNT         3822 a         4390 c         3451 g         3729 t
ORIGIN      
        1 ctattctccg gtcatctgca gcagtttttt ggcatcgtga cttctgagca acggaatgcc
       61 agcctcataa cctcttccag actaatatcc cggcgccagc gttccagtcg aaaatgacag
      121 gtatcatcca gctctactgc tgcaacaata ctgaacgagc gccgttcaac ggacgatatc
      181 agcccgtacc tgcggacaca gataccacca tttcatatcc acgattttgg acagtccttt
      241 cataaaactg acttcccttt ctgtcagcag cgtcttctga cctcaccagt attcactctg
      301 catcaccgat gccgtataca tgaacaagac aaattggcgt gatggttatg tgaaagagga
      361 gaaaatgccg atgtttcagt taggtattga tgtcagtaaa aacaatcttg atctatgcct
      421 cttacggggg acattaaagg gaagatcaaa actcgtaagc taaaaaatac cgctgatgcg
      481 gctaaaactg tggcagagtg gcttaaaaag caatccagtg aactgaacga agttcatatc
      541 atcatggaag ccactggggt atatcatgaa cctctagctt atggccttca ctttgctggc
      601 gctcagtggc agttagtcca tcattaccgc tcccggtagc ccgctcatgc tggcgccccc
      661 agacacgtag agtttctggc gtacagctaa tctttggggc aatgaaacaa attgccgccc
      721 gttgtaagtc atattcgccc tgacgcacca gaatcatacg gaccgtccgc taacggactt
      781 ccggggaaaa acgtgtattt ttggtcatcc tgtttacctc tttctcaggg agtttagtct
      841 cctagatttc cgaggcgcgg ttcagtacct gtcagtaccc tattatctgc tagtccctgc
      901 cagtaattca ccgataaatg ggaccaacag ccgattgaac tcactgcatt ttatgtgctc
      961 aggtgtgctg tccctagcca gacgttcatt gatctcatcg ctgtgtttac gtatatcctc
     1021 ctgagttaag gactgccacc ctttcccgtt agcgatccgg ttaacgccat tcagtatcgc
     1081 ctgatcatct ggcaaatcac tgcggttaca tctccttttc agataccgtg tcgcagcggc
     1141 gatcgacgcc gtttgttcta gctgttcgct cgggggaacg gtggcctgct tacgagtgga
     1201 aggtacctga tggcaaccgg cgacagtcac caggaccagt aatgacgtga tacaatttag
     1261 ttttttcaac gtattaatcc tcatattttt aaccaggtat tgtttaaccg gtttccggtt
     1321 tactatagtc ccgttattct ctgagccatt tttattacca gccccggaaa ccgaacgcta
     1381 catcaacaaa aaatggcata ctccgctcaa ggcgagccac ggaccaaaag gcagcggccg
     1441 ggtaaaatcc gtggctgtaa caaaacgcag cagtaccgtt atgattaatc cgctgactga
     1501 ggcaaacaga agcacggagg gtaatacctg ccaccccatc catgctccca gtgccgcagc
     1561 tagcttaaaa tcaccgtagc ctaacccttc tctaccggta acgaaacgaa aaagccagta
     1621 cagcccccac agacagagat acccagagac cgcccccact accgcctcct ccaagggcac
     1681 tctccccccc tgtaagctga ataacaggcc aagccagagc agggataacg tcagcacatc
     1741 cggcagcata gccgtacgac agtcaataac cgccagaacc aacagcatgg caataaaaag
     1801 ccaggcagca actaatgtca gcccgggatg aaaccggctc gctgccagaa taaataacac
     1861 tgaactcgtt atctcgacca gcggatagta aataggaata cgctcaccac aacagcgtga
     1921 tttccccttc agaaagagat agcttaacag gggaatgtta tccctgatgc tgagcggctg
     1981 aaaacagtag ggacaatgag agcgcggtat catcaggtta aagaccggct cggactcagg
     2041 ctcagaatgt gccaacaaca tcagcgcttc acgccgccac cgcctctcca gtattaccgg
     2101 cagacgatga atcacaacgt taagaaaact accgataatg ccccctagcc cccctgtgct
     2161 gatgagccac aacaaatgaa aatcagccgc aaattccggc aacctgctca catatcctcc
     2221 tgcttatcac tcccattttc acgtcaggca ttaacctgta aatcacaagt gccccggcga
     2281 agtatccctg tttatgtcat cccgggacaa aaccagccga ttaaccgtta catgtccggg
     2341 ctggtttccc tgcgcgacca catcaaatac cacggtacga atacggtacc tgcgatgcag
     2401 ttgcgtaagc cactgcatca gcgtattaaa ttcaactggt gctaaggtaa cggtcagacg
     2461 ctccccctga ggctgcaatc gggtgactga tacaccatat gcggctgaag tttgagtcac
     2521 tgccgcagat aaacctaacg gctccccctg ttctggcata ctggcctccc atttctttat
     2581 gctgggtatc tgttgtttca tccactccac tgtgtatttt tcacgggcaa ttacagtccg
     2641 ccacttatcc tcccgtacct gccagggcag ccagaagaca taatagacaa aagcgacaac
     2701 aagtaaggca acacatccgg taagcagcct tcgctcacgg ggactaatat actgcaaacg
     2761 ttttttaaga tcgttcattg ttcattctca ggatcagacg actttccacc ccgtgcggct
     2821 gctgccgtat ctcgccaggc tggatgatat atttttctcc agcgctctgc tgaaagtgtt
     2881 ccagagcctg aaaagaggct gcctgcagat ctattttcag agtctggctg ctactgtccc
     2941 aggcgagtgc ctgtaagcgt atcgttgagg tttctggcag cagttgctga agctggtgca
     3001 tcacgccccc tagttccggc atattgttac tcgtcgactg ttgcagatgt ctcagcatct
     3061 gccggtgggg atccttcacc accgtttcgc caggaaacag ctcctgatag acgcgtacgc
     3121 tctcatgctg ccaaaattca gcctgctgcc agagccggta atgtgccagc cccgcgttgc
     3181 ttccggccag aaacacacag gccaacaggg cccctgcgac ataccgccat gggcggaatc
     3241 gcgcatacca tgttccctgg cgagcaaata ttccctgtcg cagatctgcg ccatcatatc
     3301 cctcacattc agccgccaac tgcagcagat cccgtactgg ctgagttcgc cactccgtgc
     3361 tctccaagcc catgcactgt gctggcggca cggagtaaga ctcaataacc ggcggcgcag
     3421 taagagacaa gaactgtgtc agccacgaca actccaccgt catcccggcg taacgatcac
     3481 tacgaaatag ccactgaccg gcaagcctta ctgcactcca tccttcagtt gccaacggca
     3541 gcatcagtac gtcaggcagc aacctcacct cccggacccc aagttgtcca cagtacgtca
     3601 gccagtggtg catgaccgat ttttcgacca cagccacatt acacctgcca cctgtctgat
     3661 gaagaatggc gaaatgcagc tgctcaatat ctgttgccaa ctgctcttcc agaagaaagg
     3721 ggagcacctg caaacgctga cgccacgtac ggtgtggcag cgtgacctga tggaaggcaa
     3781 ttctgtctga cggaaccagc acccaagcgg gataacgagg cagcagacct gctaacagcg
     3841 gatctgtttc tccgactgcc acgctgccgc cccctgtctc ctccggcgaa accaacctcc
     3901 acaataaagg ctgcgtgtca tcctccggca gtcgcaaaaa aagccggcct gcctcgacat
     3961 gtttgttatt cttgttcagg gtacctccct caccgttaat ccatactgtc gctgaatgct
     4021 ccggaaacgc tcctctctcc actgcattaa agtctgctgg ctgaacaggt gctccccaga
     4081 gcgcacatgt aaccggacca aaaaatagtt gctgctaaca gccagtaccg atatggctgt
     4141 actagtgtcg atgcccttaa gctgtggcga gtcaagaaac gctgccacgc tatcccaacc
     4201 ggtacggggt cgccgcagca gtaatttcgt caccaaaaga ctgttcggtt tcgtcagaaa
     4261 gagtgcagcc agcaacggcg cctgtgaatc cagcagagta ttcacattca ctgacaggtc
     4321 tgacgtgggc agtacacaaa catacggtaa aagtttgcgg taaagtctga catctattcc
     4381 ccggatgagg cgcaactcac tgacgtcctg catcggttgg ctggccgcaa gatagggggg
     4441 ctccatcccc atatacacct cgtcttcagc cccctcctta actggttcgt catcacgatc
     4501 gatccagtca cgcagagctg ctgtcacttg cctggcttgt ataatatcga cttgcaggtt
     4561 gattaacagc tgctgaaaaa ttctggcggc ataaggcaca ccggctagat ccactgctcc
     4621 ataattaatg gcattaagat taaaacaggc ctgtgcatcc actatccgtc cccgaacatc
     4681 tcctccgccg gtttcaaact gtagttctgg ctgcgcccag ttttgtgcca gatgcgtttt
     4741 ccggggcgat gcctgtctgc tcctctgtaa tatttgtatt gccatctcct ctgcagcata
     4801 gccatcccac tttgcctgca gctgatccag ctgcgccacc gtacgccaaa atgtcctgcc
     4861 gttacgctcc gcgatcgcag ccgcgatcgt caccattaac gacaagatca gcaatacaac
     4921 cagaagcgcc acgccctgtt cccgcgactt cattccgcgg tctccttact tacaagaaac
     4981 agacgggtaa gttcagcaaa atcatccata accagcgtca cctcaacagc ctgcggcagg
     5041 atgctggtac tgtcccagcg cgcctgccaa gtgccgttga caaaaaaacg cagacgaaat
     5101 gcgtgaaccc cctccagcac caccctgaca tccggagcaa taccagaggg gtgatcgaca
     5161 taaaagtagc tcaggcgttc taacttttgc tgtcgcagcc ggtaacctac ccactgtaac
     5221 tccgggcgtg gtagcatccc ggcgggatta agccaactgt ttcgcgtaaa ggaaatcccc
     5281 cagtcatcgc ttttcagcag atgaggagca gcgagcagca ggccttcact cccccggcta
     5341 tgtcttggga tcatctgcat aagatctcgc tctatctggc tgccccccct ctgcagctca
     5401 gccaaacgcc gtactttatc ttgggacagc tcgtgcgcgc gtattccgct ctgtagtacc
     5461 tgaaatgcgc tgatgctcag cgctgcaaaa acagccagtg ccaacagcat ttccagtagc
     5521 gtaaaacctt tcacccgctg ctgactcatt cacgaaccac actaccacgc aaggaaacta
     5581 aggcggatgt ctcctctctg gtacgccgta cctctacctc taggatgcgt tgctctggta
     5641 tgtcgctgtc cctcccgtgc cagcgtaaat accattctct gccagcgaag ctgatcgtct
     5701 tctcgtccca gccgtttttc ggccagattt tctcgagctg cagttgcacc atctggttgt
     5761 cagccaacca gccagccaac accttctctt ccatcggccc gatccctgcg gcctgctgag
     5821 tcgtggactg aattagagca attcctgcca gcgcaaacac aaccaaggcc accatgactt
     5881 ctagtaatgt cattccttct tgcctcttca caactcgacc tcaccttcaa atagggtcat
     5941 caggccgttc tcatcaacct gcagcccagt aacggcctcc cctcctacca gcctaaagag
     6001 aaggctgaac ggcgtcacct cacctccagg cagcaacaaa atatcgggcc ctgtcccgtc
     6061 attatgctca ggcatccact gcagacctct gagatactgc agatcaagac gcacatcatc
     6121 cggaagtttg ccctccatgg cagcacgccg cggttgccaa gtttgccaga catacccttg
     6181 ccaaacatcc ccccccactg atgccgggaa gctttcagct gttacgcgct gcaaaacctt
     6241 aaatgtccac ccgtcagacc ggacctggat cccaatcagc aagccgtcct gcatgcttct
     6301 atccaaggca taatccagct gcgcctgcag acgctcccgc tgttgttgca aatgattctg
     6361 cgctgagtca ggaaatgaca ttagtaccaa ccctgcagta atgcccatca acaaaaggat
     6421 cagcatcatc tccagtattg tgaatccgcg ctgactcacg gctgcgtgtg accgggcgtc
     6481 cagttaccaa tatcatcttc cgtgttcggt ataccatccg ggccgggaga gaagatgtca
     6541 atattgctgt actgaccagg atttaacagc tgataatccc ccccccaggg atcttgaggc
     6601 aaacgacgta tataaccatc ttgccgatag ttacggggct caggctgcac tgtaggttta
     6661 ctgaccagtg cccgtagccc ctgctccgtt gttgggtaac ggttgttatc taaccgatac
     6721 atatcgagcg cgctttccag cgcaaccaga tcgctaacca ctttctgccg atcggcctta
     6781 tccttatttc ccatcagatt cgggaccacc agactggcca gaactcccaa gatcacaatt
     6841 accaccatta tttccaacaa ggtaaaacca cgctgatgct gttttcgcat gtttttctcc
     6901 gttgactatt aaagaagaat tcaaatattc atcatattgt tgagttgcag gagcggttgc
     6961 agaattgcca gaacaataaa caagacgacg ccagccatcc ccaccaccaa cattggttca
     7021 aacaatccca gcgccagctg catctgtgcg ctgaattcac gatcctgatt atcggcggcc
     7081 cgttccagca tgctgtcgag ttcgccacta ttttcaccgc tggcaatcat gtgccgcatc
     7141 atggacggaa atagcgaggt ctgctccagc gcctgatgca ggctgacccc ttcccgaacc
     7201 aattcggctg cggtggctaa ctgatgacgg gcccagtcat tactcaacac gtctccactg
     7261 atgtacatag cctggagtag cggcaccgca ctggcattaa ggatgctcag cgtgcgggca
     7321 taacgtgcgg tattcagccc acgcgatata cgccccacga tcggcaggcg cagcagcaga
     7381 tgatgaaaag ccagacgccg ggagggcttg tgtaacatca tacgccccgc tatccagccc
     7441 gtggctgcgg ctagcagcag ccacgggccc caagtctggc agacctcagt cagccccatc
     7501 agtacgcggg tagacagagg aagggtctgt ttcatatgaa taaactgctc aaccactttc
     7561 ggcacaacag cagtaagcag aatggcaatc acccctaccg ctaccaacgt caggacacag
     7621 ggatagatca gtgcctggag cagacggttt cgcatgatct ggcgttgctc ggtatagtca
     7681 gccagacgac taagtaccac atccaagcga ccggatgttt cacccgccgc caccatcgca
     7741 caataaagac gctcaaacgt accaggaaac attcccatcg ccgtggccaa ggaatgtccc
     7801 tcaaggaccc tagcgcgcac ggcagcgatt aaatcccgct ggcgaggttt ctcactctgc
     7861 ctcgacagcg cgtccagcgc ctcttcaagc ggcagtgatg cggccaccaa ggtggataac
     7921 tgccgtgtca ataatgcaag ctcggcgctg ctcaccccac gaggacgaaa tcgaaggata
     7981 ctccgcggtg agtgctggtg atggagctgt ttctcttcca gcctaactgg aataagcgcc
     8041 tgctcacgca gcaactggcg ggcatgtcga gctgaatccg cctccagaac gccacggcgt
     8101 tttctaccgt gaatatccga tgcctgataa tgaaacaacg ccatatcatt cctcttcctg
     8161 caacctgctt tcagtaacgc gaaccacctc ctcccaactg gtctcaccgg ctagcacctt
     8221 ttgtcgcccg tcgccacgca gcgtcagata gcgcccccca gccaagcggg taatactcag
     8281 ctctccttcc ccctgatata ttgccatccg gatgcgatcg tcaatcagca acagttcgtg
     8341 gagcccggta cgcccgtgat agccagtaaa actgcagtgc tgacacccgg caggctgcca
     8401 aatcgtggtt tccgggggaa tatccatgtc cttcgccagc tcagcactgg cctgacagga
     8461 ctgacgacag cgcggacaca gcgtgcgaac tagtcgttgg gctagaacag ccaacaatga
     8521 ggtggaaagc agaaaggggg ggatgcccat atcctgcagg cgcgataacg cgcctgcagc
     8581 gctgttcgta tgtaacgttg acagcaccag atgcccagtc agtgaggcct gtacagcaat
     8641 ctgggctgtt tcactgtcac ggatctcccc tacaagcacc acatctggat cctgacgtaa
     8701 aatagcacgc aggctcctgg caaaagtcat gtccaccttc gggttgacct gggtctgact
     8761 gataccgtcc agttcatatt caacaggatc ctctactgtc atgatgttgc ggtcctgtgg
     8821 attcagccgg cttaacgcag cataaagcgt cgtgcttttc cccgacccag ttggcccagt
     8881 caccaaaata atgccgtgag gacggcggat cagcgcatca atgtgtctct gattctgcgg
     8941 cgacatgccg agtgcagcca gatcgagact aacgctgttt ttgtctagta aacgcagcac
     9001 gatgcgttca ccatggttcg aaggcaacgt tgaaacacgc acatccacag cccgtccgcc
     9061 tatacgcagc accatgcgac catcttgggg aacgcgtttt tcggcaatat ccagccgagc
     9121 cataacttta atgcgtgaaa tcagtaacga agccagattc cggcgtaaac ggaggatctc
     9181 ccggagaacg ccatcaacgc gaaagcgaac ctgtagatgg cgttcgtacg tttcaatatg
     9241 aatatctgag gctttctctt taattgcctc agtcagcatg gcgttaatca ggcggataat
     9301 aggcgcatcg tcattcgcat ccagtaagtc ctcactgtca ggcaactccc ccgcgacggt
     9361 ataaaaatcc atttcattac caatatcagc catcatttgt cgcgcttcat cagaatcacg
     9421 ctgatagcag ctcaccagct gtttttcaaa tgcctcagcc gacagagttg tgacattcag
     9481 aggaccttca gctaccctgc gaacctctag cagggcactg gcaggtgtat cctccatgca
     9541 aaacacttgg ttcccttcct gttcacgcag caacaaaatt cgctgtgtac gggaaaagga
     9601 aaaagggaga agggggcgtg attcactgac gttctgtata ctgctcattg catcccccgc
     9661 gcataaaacg cagcaatcgc cgttttcact ttccgaaaag tcgtatcgtc ccgatctcgg
     9721 gatagcttga gctgttcctc attcagcgtc agcgccattg tttctttacc acgcgactgc
     9781 acctgctcct gattaaacga caggtatttt tcggccgaag catgacgaaa tccgtcgcgt
     9841 tcacgaataa ttgttggccg aatgaacaac atcagattac gcttggctgc atgttcactt
     9901 tttgagctaa aaagatgtcc cagtactggg atatccccca gcagaggtac tttactctca
     9961 ctccctctga tcgacttatc cagtaatccg ccgaccacca ccgtttcgcc attccctacc
    10021 agcatagcat tggtcactgt ccgggtattg aaggttgccc ccaagtcagt agtggtagct
    10081 actgctgtat ctgccacacc ggacacctcc tgctctatct ccagtaacac tgaatcaccc
    10141 tcgttgatct ggggttttac cctgagtttg atccccaccg ttttacgctc gacggtgtta
    10201 aaaatattgt ccccagaggt ggtctgtgaa cccgaaagca ccggaacttc ctgaccaaca
    10261 ttgaacgtgg cctccatatt gtccagcgta acaatactgg gggttgccaa gatatcattt
    10321 ttgctacttg tggagagcgc cgtcagcagc atcgcccagt ttccctgata aaaccctgct
    10381 gcaatcccgt taaacatgct caaggtattg ctctgatcgc tgtcagaaat atcgctctga
    10441 cgggtctgaa ccatcgtggt aatgggtaaa ccagtactgg tgaactgcgt cacaccggag
    10501 tgtttattca cccactgaat ccccagattc aacccgtcag catcctgtac ttcagcaatg
    10561 atggcttcca ccaggacctg aggacgacga atatccaact gcgaaattac attttccaag
    10621 tcgcggatga cgtcaggagc ccctgtcagg atcaaggcat tggtttgttc gtgggattta
    10681 atcgtcaccc cttttcgcag agcttccgtt gacgagctct ttttagcgtc gttttcaata
    10741 ttgctgctga tgcccgtcag gacttccact aaatccttcg ccttcgcata tttcaggtaa
    10801 atcaccttcg tattaccctg aacatcctcc ttacggtcca gttgcttaat catggagatg
    10861 atacgctgtt gggagattgg ctctccgctg acgagaactg agtttgtccg ctcgtcagca
    10921 accagcttcg ctgtcatcca ggctggcatg gctgtcttat cactttcttt agtcagttcc
    10981 gtgacaagtc tggctacatc cgttgcagag gcgtaggtaa gtgggaccgt ggcaacgctg
    11041 cgattgccct ccttatccac ccgttcaaca atctccatca gccgtttcac cacggcagca
    11101 cgaccagtca tcagcagaac gttagagggt tcataatgca ctacgcttcc tgcgccagca
    11161 ttatcattga gctgacgcag caagggcgcc aagtctctgg ctgccacgtt actgacgggg
    11221 accacccggg taacaacctc atcaccagtc ccagggctgg catcactagc taccggcacc
    11281 gccgacgttt tagcatcttt tgaacgcacc actttcagta taccgttgtg catatcgacc
    11341 acggcaaaac cataaacatc cagcacgctg agaaagaact gataatattg ttcctcgttc
    11401 agcatatcgt agctgcgtac agtgatggtc ccctgcacat caggattaat tattaccgtt
    11461 ttgtgcagat ttttgctgac agtattaatg aactcctgaa tatccgtatc tttaaagctg
    11521 gccgaaaatt cagcccccca tcccggaatg gcactcagaa atactgtcgt gaatacaaat
    11581 atcgacaagc gcccgttcaa ccatttcctg aacaatcagt tctctcctac agcaataaag
    11641 gcatcataca gttgaccatc acgttcgacg gttattttta tttctttaag ttctgtcaat
    11701 tgtttcatta tctgcttcgc ctgtctggta tcacgcagtt ctgaaccatt aagcaatacg
    11761 gccagatcgt tatcatgcaa cccagcatta taaaataacg aactggcctt accggggctc
    11821 aagcgataac cgcttaatgt attattcacc gtgacaggtg aaaggctcat tatgtcaaaa
    11881 atatttttta cccgaaaatt agcttgggtt gtcacgggtc cgactattaa attattatcg
    11941 tcctgtgcat tcctttttgc tggattatca tatcgcagcg gcagtgatgc attttttccc
    12001 tgatagttaa taacaatatg atcgctaaaa atagcactaa tcgtagcatc gtaaccaggt
    12061 actttttcgc cgatgcctag actgaactgt ctgttattat gtgcaaatat ggcaacggaa
    12121 tgctctgtaa ccgaactgta aagtatacct ctcacatcaa gatccccccc tgaaagcagg
    12181 tcatcagaca gggaagatgt tgttttctgc actttccctt ccgataacgg cggggatgcc
    12241 gtgccgaata acgtaaacac aaaattttct tctgtgtgct catcggttac cgctgtttcc
    12301 gacacgctaa catgggataa atcggacgcc gatgtcgccg gagtcagcca gaaatggcgt
    12361 ataaccgaga ccagctgata tccggcaatg agtaaaatca cgcacaacac ccagtttggc
    12421 gtaagcattt taagtgcgat atcttttatg aataagttcc ggtctctccg ggatgataga
    12481 aagaacaaca taaaacaccg ggcgctattc taggttttga tgaacggaac gaatgtatta
    12541 ccgatgcgac ctggtggccg caccgtcttc cggattattt atttacaacc ctcatcgacc
    12601 tcggtctgct gaagtccagg tactgcccgt tccaagcttt aacttttttc atatcagctg
    12661 gattcacatt acccacaaac tcaccaatcc tgttatagga caggttatcc cagtcagaga
    12721 gcagcacttt cgctttggcc ccgctgtcga catagacttc ctggcctgcg atccagtctg
    12781 gcagactgga tcctgaccgc tggccaacgg ggagacagta acgctgggca gtcttcacag
    12841 aaacgataca tccctcgttt tcttttgccg gaagtgcccg accatttaca gtataagtca
    12901 gaccttctgg tgctggtgtg agtgttcggg tatccaatac tttgttatta cagactaaaa
    12961 tcgcctgagt gggctgactt tcagtcggca cgttgatatg gaacttgttc atcactgaac
    13021 tgttcacacg attattagcc agtttgaatc gtagctgccc ttctctagta tccacctgca
    13081 gctggcagtc attactggac aggttctcac tgtcatcaga ataagtgaaa ccatacgcac
    13141 cgtacattgc aggatagatg aagctagaca aagtgccttt gggatcgtaa taacccacca
    13201 gtgtcgtcac cggaacgcca aacttctctg gcttacgcgc ttcgcgggta ttcaccacgt
    13261 cactttcttt ccaggtctgc ccatcggaaa caaagctctt cttatatccc ttggaaatta
    13321 tcttttcacc accgttgata aacagatggc tgttataacc agcttcatga tcgagtgtca
    13381 gaatacggcc tttattctcc gcggaagctg caggggcatg aatatctcta gtccagttgc
    13441 cgtccgacat atgtactttg acaataggat attctgccat catctcagcc attttgtttt
    13501 cactgagatc cctgacagaa gcagtaatct gctcaaacct gtcaatggtg tgttcatacg
    13561 gttccatttt atgctcatcg gcattccact tgctgaatcc agtggaggaa aggctatcga
    13621 acacagcttt attttcaaaa aagcgctgga tgatagccga tgaatttggc gtatacattg
    13681 tgaaacggtt tgcgtcggag aaaggactgc ctcccgccat ggcgtcaaag ccaaacgagt
    13741 gaccgtcgaa cggctcctgg cactgctcgt caagacaact tttccggttt gtctgggctg
    13801 gataaaaatt aggtatgaaa cgttttttat cgctatccca gccccaagtc gagttgatct
    13861 ggtcagcgct gcgatgcacg gaccccctga agccacccac ataatgacca agaccgaagt
    13921 tatgcccgac ttcatggctg aattcattac ccaaggtaga gtctagggta acaattcccc
    13981 ctccaccgga accaccgtgc acctgtactc cattggagta gttaccgcgg tttgtatgtg
    14041 cggtcagttg cgcagccaca taaggatggc tgctttctcc tgaaccagca gtgctgttaa
    14101 taccatagtt ggcattatca ataccatgag agatcagctc cttaccgata ctctgtcgca
    14161 tcgtgccgct atgccatcca ccctcaccgg gatctgcatc tgtcagcaat gtaccggtag
    14221 gtagcatgac ttccttcaga tgaagtggag cataattgtt gacgatcata cgactgaccg
    14281 ggatcgtctg gaaatattcc cgatgcgcct ctttgtcctt ggcaaaatca aagcgccccc
    14341 ggggagtggt cagcatcccg atatcgatgg tatgcaacaa cagctcccct ggtgctccaa
    14401 cattgatgtc atttaggcta ccactcaggt taccttgttt aatcaccaag tttaacccgg
    14461 gcacgatcca gtgcgcaggc agttcagcac tccaggtatg ctgagcataa gcgattcggt
    14521 tattctccag ctcaccggag cggaaccact gaccatttac atatttgaac agcagggtgt
    14581 tacccaccga gagcgtgacc tttcgatctc cataaaaaac cgttgaatta tagccggcat
    14641 acgaaacaaa gcgaaccatc ttaccttcaa gctcggctcc ctgcggcaaa tacatgtctc
    14701 ttatccaacg accatttgct gtctggatct ccaccagtgc attatttgcc agatagcttt
    14761 taatagaact cccgctggca tcactgagtt tgtttacttc agcaaccgta ttaatgatct
    14821 ttttcgttcc attctgagga gtaaaatcga taccatcagc aggaacacca tctaggtggt
    14881 agacggtatc cggtagtgag gagggaggcg aaagggttaa cgtaccgaga attttatcat
    14941 tagcatctcg ggcttccacc tgaacaggtg ttttatcatc agccttcacc agacggacca
    15001 acagcaaact tttccgcagg ctggtcagat gttgttgact ttccccttct ttaggatggg
    15061 cagataaaat ctggctctgt gcaaatttca cctcagcggc cagtcccccc tgcagatcat
    15121 ttacgggctg ggtggtattg aaataaatgg ctgaattatt atcagcagca gttgcagcaa
    15181 atgctcccat cgccagagag gcaaggatca tgcatgacag atactttaat ttcataagtg
    15241 tactccatct ttcattttag atttcatgca gccggatata atagaagtgt taaattttat
    15301 tttttcagga gtgattcact cattcaccag tgttgatttt tataacacac ttatatctac
    15361 ttcagaaaga taacctgata aaaaatatta ac
//
LOCUS       DAZFWP020000116        15321 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFWP020000116 DAZFWP020000000
VERSION     DAZFWP020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.116"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(172..1083)
                     /gene="gsiD"
                     /locus_tag="ACHK0Z_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             complement(172..1083)
                     /gene="gsiD"
                     /locus_tag="ACHK0Z_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU4503486.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1086..2006)
                     /gene="gsiC"
                     /locus_tag="ACHK0Z_RS17640"
                     /old_locus_tag="pgaptmp_003528"
     CDS             complement(1086..2006)
                     /gene="gsiC"
                     /locus_tag="ACHK0Z_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU4503487.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2024..3562)
                     /gene="gsiB"
                     /locus_tag="ACHK0Z_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             complement(2024..3562)
                     /gene="gsiB"
                     /locus_tag="ACHK0Z_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU4503488.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3582..5453)
                     /gene="gsiA"
                     /locus_tag="ACHK0Z_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             complement(3582..5453)
                     /gene="gsiA"
                     /locus_tag="ACHK0Z_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU4503489.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5440..6405)
                     /gene="iaaA"
                     /locus_tag="ACHK0Z_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             complement(5440..6405)
                     /gene="iaaA"
                     /locus_tag="ACHK0Z_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4503490.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6609..7844
                     /gene="moeA"
                     /locus_tag="ACHK0Z_RS17660"
                     /old_locus_tag="pgaptmp_003532"
     CDS             6609..7844
                     /gene="moeA"
                     /locus_tag="ACHK0Z_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU4503491.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASNV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7844..8593
                     /gene="moeB"
                     /locus_tag="ACHK0Z_RS17665"
                     /old_locus_tag="pgaptmp_003533"
     CDS             7844..8593
                     /gene="moeB"
                     /locus_tag="ACHK0Z_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU4503492.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8669..9331)
                     /gene="fsa"
                     /locus_tag="ACHK0Z_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             complement(8669..9331)
                     /gene="fsa"
                     /locus_tag="ACHK0Z_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4503493.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9462..10361
                     /gene="ybiY"
                     /locus_tag="ACHK0Z_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             9462..10361
                     /gene="ybiY"
                     /locus_tag="ACHK0Z_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU4503494.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10367..12799
                     /gene="ybiW"
                     /locus_tag="ACHK0Z_RS17680"
                     /old_locus_tag="pgaptmp_003536"
     CDS             10367..12799
                     /gene="ybiW"
                     /locus_tag="ACHK0Z_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGU4503495.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12945..13760
                     /gene="ybiV"
                     /locus_tag="ACHK0Z_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             12945..13760
                     /gene="ybiV"
                     /locus_tag="ACHK0Z_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU4503496.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13912..15177
                     /gene="ybiU"
                     /locus_tag="ACHK0Z_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             13912..15177
                     /gene="ybiU"
                     /locus_tag="ACHK0Z_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU4503497.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3705 a         4237 c         3906 g         3473 t
ORIGIN      
        1 acatttaaca aaataactat ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg
       61 ctttttaacc aaattacccg attctacaaa atcagcagaa agttacaaca cttgcttagg
      121 gcaataattt cctgtgaggt gattaccctt tcacgcaata ttcaaacgta actatccttt
      181 aattttcgga tccagcgcat cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt
      241 cagaaaaata gccagggccg gaaaaacagc gacatgcggc gcgataacca tatccgctcg
      301 tgcctcattg agcattgctc cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa
      361 tgacaggctg gcggcagaga taatcgaggt gccaatgcgc atggtgaaaa acaccacgat
      421 agacgagacg gtcccaggca agatatgacg caataaaatg gtcatatcgc tggcaccaat
      481 actgcgtgct gactcaataa aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag
      541 tcgggcaaac gcggggatgg aaaaaatggc gactgcgata atcacgttag caatgccgct
      601 tcccaacacc gcaacaacag cgatcgccag taaaataccc gggaaggcaa acagcacatc
      661 gcaaatgcgc atgatcaacc gatcccacca gccttcataa tatccagcca acaagcccag
      721 caacgtcccg atcgccgcac cgataaacac ggcaaacacg cccgccgcca gcgagatttg
      781 cgcaccaacc aggacacggc tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg
      841 ctgcaaagaa ggtccgttat tcaggttgtc ataatcaaaa taattttcgg cgtcataggg
      901 agcgatccag cgtgcaaaaa tggccaccac aatcaataaa ataacgaata atgcggcggt
      961 catcgccata tgctgacggc gaaatcgtcg ccagaattca tgccacggtg tacgcacctg
     1021 gtcaggtttg accagtggca tggcgtttaa caccgcctga cgtcgccagt taaatagtcg
     1081 catccttact tgtacctgat agccgggtta atggcggcgt aaagcacatc caccactaag
     1141 ttgataagaa taaattccag cgagaaaagc agaatttccg cctgaatcac cgggtaatcg
     1201 cgcatttcta cggagtcaac gagtaagcgt ccaagtccgg gccagttgaa aactttctcc
     1261 accacgatag atccaccgag caaaaagcca aactgtaagc ccatcatggt cactaccggg
     1321 atcatcgcgt tgcgtagccc gtgtttgagg acaacccagg tttcgctcac ccctttcgcc
     1381 ctcgcggtac gcatataatc ttcgcttaaa acgtcgacaa atgacgcacg ggtaaagcgc
     1441 gccatcacgg cggccactgc cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa
     1501 ctgtctgctc ccacagtagg cagccagccc agttcaacgg agaatacctg aattaaaagc
     1561 atccccagag caaatgccgg aaacgagatc cccgacaccg caatggtcat actcaatcga
     1621 tccggccaac ggttacgcca gacggcggcg ataattcccg ccgccatacc aaatatcacc
     1681 gcccagacca tactggttat ggtcagccac agcgttggca taaagcggct ggcaatctca
     1741 tcggcaactg gacgacgcga caccatggac aggccaaaat ccccctgcac agcattgctg
     1801 atatagtgcc agaactggtg atacagcggc tgatccaacc ccagctgctg acgcaccagt
     1861 tctataacct gcgcatcagc ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc
     1921 atatggacaa ataaaaacac cagcaccgag acgataaaca gcgtcggaat caaccccagt
     1981 aatcgtttga taacgtaatt aagcattcca ctccctgcgt tgcttattgc aaatccgcat
     2041 cttcaaagct gaagccggtg tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg
     2101 ccgacaccag tttttctacc accagcggga tccacggcga ctcttgccag atgatatcct
     2161 gcgccgcctt atataaacgg gtcttttccg ccggatcatt agttttcagt gcctgagcca
     2221 ggaagtcatc cacctgttta ttgctgtaaa acgcggtatt aaacagcgtc ggtggccagt
     2281 tttgcgaggc aaacaacggc gatagtgccc agtcggcttc gccggttgaa gccgaccagc
     2341 cagtgtagaa catccgcacg ccgctctctt tttgcccttt accttcaact tctgccgccc
     2401 gctgtccggc atccatcgca gtcacctggg ctttaatccc gacctgcgct aactgctgct
     2461 gggtaaattg cagcactttc tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac
     2521 tgaaaccgtt gggatatccc gcctctttca gtaattcgcg cgctttcact ggatcgtaag
     2581 gccacggttt ataactttgc gcgtaggcga tacttggcgg taccacacca gtagctggcg
     2641 ttgcatagcc cgcaaaggca actttcacca gcgccggacg gttaatggcg taattcagcg
     2701 cctcacggac cttcgggtta tcgaacggct tttgcgtcac gttcatactg atataacgct
     2761 gcataattga cggactggcc atcaactcga tatttttgtt tttctccagc agtgtggcct
     2821 gctcgtaggg aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg
     2881 tgttgttatc cgccaccgga cgccaggtta tgctgtccag tttgggcaat cctggctgcc
     2941 agtaacccgc gaattttttc accttcacaa aatcggtctg attccaggta tccagttcat
     3001 acggtccggt tcccaccgga tgaaaaccaa tctccttgcc atatttttcc agcgctgccg
     3061 gtgaaatcat cgcggtcgcc ggatgggcaa gaatattaat aaacgctgag aacggctgtt
     3121 tgagagtaat ctttactgtt gttggatcga tcgcctccgt tttggcaata ttcttataca
     3181 ggttatagcg tttaagatga ttcgccggat cgctggcccg gtccagattc gcttttaccg
     3241 ccgctgcgtt gaaatcggtg ccatcctgga atttaattcc ttcccgcaat ttcacggtgt
     3301 atgtaaggcc gtcatcggaa acggtataac tctccgccag cacgtttttc agtttcatct
     3361 ctttatccag accaaacagc ccctggtaaa acgatttcgc tacggcctga gataacgtgt
     3421 catttgcgtc atacggatcg agcgtggtga agttcgatcc taccgccacc accacatctt
     3481 tggcagcgaa tgcacaagat gccatcaacg ctgtcgcaat gcccaacgcc actaacccac
     3541 tacggtgtac agctcttgcc attttattct ccgcctgaat gttatctacg catgaatgcg
     3601 tattctgatt gtggttgtgc gacgtaatgc cccggcccga cgcattgcaa cgagacggct
     3661 gccacctctt cgccacgcag atgaatattg ctgggaagat cgtccgacag cagtacacgc
     3721 tgcggtcgtt gtcgggacgg ttcagcgacc ggaactgccg ccagtaattt acgcgtataa
     3781 ggatgctgcg ggttttcgaa gaccgcgcgc cgtggaccaa tttcaacaat ttgtccgaga
     3841 tacatcaccg ccacacgatg actaatccgc tctaccacgg ccatatcgtg ggagataaac
     3901 agatacgcaa tgccgaaatc acgctggaga tcgagcaaca agttgataat ctgcccgcga
     3961 atagaaacat ccagcgccga aacggcttcg tcggcaatga tcacttttgg attcaatgcc
     4021 aacgcgcgag caatgcagat gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc
     4081 caggcatgtt caggtaacag gcccacgcgc tccagcaacc acgcaacgcg tgcagccgcg
     4141 tctttacctg gcaataaacc gtgtacacgc agcggttcga taatcgaatc accgatggtc
     4201 tgacgtgggt ccagcgaagc gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat
     4261 gcctgaagtt tgccgggtga caaggtatcg attctctgac cgttaaagat aatttcgccg
     4321 ccctgcgatt cgaccaggcg cagcaacgcc cgcccggtag tggatttacc gctgccagac
     4381 tcgcccacca gcgatagcgt ttcgccaggc cagagatcaa aactgacttt ctcaacggca
     4441 tgcacttccc gcgttacgcg attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga
     4501 ttacgcactc gtaaaacagg ttcgccatcc accaccgttt tctgctcgat ggggggggcc
     4561 tgtttcgctg gatgttcaag cgatatcaac gggaaacgtc ggggataatc taaccctttc
     4621 atcgcaccaa gttgcggaac agcagctaac agcgcacggg tgtaaggatg ttgcggtgca
     4681 tgaaaaatct gttcgacggt acccgtttcc accgcctcgc cctgatacat caccagtacc
     4741 cgatcggcaa tctctgccac cacgcccata tcgtgagtga taaagataac gcccatcgac
     4801 atctcttttt gcaatacttt gattaattgc aggatctgcg cctgaatagt gacatccagc
     4861 gcggtggttg gctcatcggc aatcagcacc gccgggcggc atgacagcgc catcgcaatc
     4921 atcactcgct ggcgcatccc gccagagagt tgatgcggat aacgtgaaag aatggtttgt
     4981 gcctcaggaa tgcgtacctg atccagcatc cgcttcgcct cgaccatcgc ttcttcacga
     5041 ctggcgttct gatgcagacg aattgattcg gcaatctgtt cacccacagt aaataccggg
     5101 ttcagcgatg tcatcggctc ctgaaatatc atcgccatat ccgcaccgcg aacatggcgc
     5161 atttgtgcag cgctctgctc gctaagttca atcacttcgc gactgcgccg ctgcaacagc
     5221 attttatcgc actgtactaa accgcccgcc tgttccaaca ggcgcatcaa tgccaacgca
     5281 gtcactgact taccggagcc ggattcgcca acaattgcca gcgtctcacc gcgttgcaga
     5341 ctaaaagaga gattgcggac cgcagctatt ttctgctggt cctgcataaa ggcaatattc
     5401 agattttcaa ccgccagcac attaccggca tcaagttcat cactgtgtgg caacggtgtc
     5461 ccctttttca cggtagatac cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata
     5521 cattccttcg gtgttaaacg gtagcgcgac attcccttca tggtcgatag cgattaagcc
     5581 accgctaccg ccaagcgcag ggagtttttc cattactacc cgctcgcagg cttccgcgag
     5641 acttaatccg ccgtaatcca ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa
     5701 gacttcgccc gtgccggtac aagaaaccgc cacactggcg ttattggcgt agcatccggc
     5761 acccactaag ggactatcgc caactcgtcc gggtaattta ttggtcattc cgcctgtgga
     5821 cgtggctgcc gccaaattgc cgtctaaatc caacgccacg gcccccacgg tgcccatttt
     5881 ctgtttttca tccagtggcg caccgctatg gtcgaggacg gttgcccctt ccttgcgcgc
     5941 tgccagtagt tgttcataac gcaaaggcgt ggagaaaatc tccggcgaga cgcgctccat
     6001 gccacgagca aacgcaaaat tttctgcccc ttcgccaatc atcatcacat gcgggctttg
     6061 ctccatcacc agccgggcgg caagaaccgg attacgcaga tgactaacgc ccgccaccgc
     6121 accggctttc agggtgttac catccatcac acaggcgtcc agttcatggg tttcatcacg
     6181 cgtaaagaca gcgccaattc cggcgttaaa cagtggacac tcttccagca gacgcaccgc
     6241 ttccgtcacc acatccagcg cactttcgcc cgcttccagc attttctgcc cggtttcaac
     6301 aatggcagac aacgcctcaa tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct
     6361 aattgcacct gcgccaccat gaattgcaat gactgctttg cccatagtat tcgtccatta
     6421 atggtggctc gccacttttg ctataaatat cagaattttt atgaattact cattcaattt
     6481 ttgaatatag aaagatggat atggcatgta aaggcaggag ccaacgctac gcccgaggtg
     6541 gcatacagtc tgtggcgttt ttctgacata ataggcaaat tcgattttgc ctccgcagga
     6601 gtgttttcat ggaatttacc accggattga tgtcgctcga caccgcgctt aatgagatgc
     6661 tttctcgcgt caccccactg accgcccagg aaacgctgcc actggtacag tgttttggtc
     6721 gtattctggc gagcaatgtc gtttcgccgc ttgatgttcc ggggtttgat aactccgcaa
     6781 tggacggcta cgcggtgcgt ttagccgata ttgcctccag gcaaccgctg cccgttgccg
     6841 gtaaatcctt tgccggtcag ccgtaccatg gtgaatggcc tgcgggcacc tgcattcgta
     6901 ttatgaccgg tgcgccggtg ccggaaggct gcgaagcggt ggtgatgcag gagcagactg
     6961 aacaaacgga caatggcgtg cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc
     7021 gtcgcggtga agatatctct gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta
     7081 ccgcagagct gccagtaatt gcttcactgg ggattgccga agttccggtg attcgtaaag
     7141 tgcgtgtagc gcttttttct accggtgatg aactccagtt gcccggtcag ccgctgggcg
     7201 acggccaaat ctacgacacc aaccgtctcg ccgtacacct gatgttagaa cagctgggtt
     7261 gcgaggtaat caacttaggg attatccgcg acgatcccca tgccctgcgc gccgcattta
     7321 ttgaagccga cagccaggcg gatgtagtga tcagttccgg cggtgtttca gtgggtgaag
     7381 cggattacac caaaacgatt cttgaagagc tgggggagat cgccttctgg aagctggcga
     7441 ttaaaccagg taaaccgttc gcgttcggta aactcagcaa tagctggttc tgcggcctgc
     7501 cgggcaaccc ggtttcagcg acgctgacct tctatcaact ggtacagcct ttgctggcaa
     7561 aactaagcgg caacaccgcc agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc
     7621 gcctgaaaaa aacgccagga cgtcttgatt tccagcgcgg cgtgctgcaa cgcaacgccg
     7681 acggtgaact tgaagtgacg accaccggac atcagggttc acatatattt agctccttta
     7741 gtctcggcaa ctgctttatc gtgctggaac gcgatcgcgg caatgtggac gtgggcgaat
     7801 gggtggaagt agaaccgttt aacgcgttgt tcggaggcct gtaatggcgg aactcagcga
     7861 tcaggagatg ctgcgctaca accggcaaat cattctgcgc ggttttgatt ttgacggtca
     7921 ggaggcgcta aaagattctc gtgtgctggt agtcggcctc ggtggactcg gctgcgcagc
     7981 ttcgcagtat ctggcaagcg ccggtgtcgg caacctgacg ctgctcgact tcgacacggt
     8041 ttcgctctcg aatctgcaac gccagacgct gcacagtgat gctacggtcg ggcaaccgaa
     8101 ggtagaatcc gcccgtgacg ccatgacgcg gatcaaccca catatcgcga ttacgccagt
     8161 caatgcactg cttgatgacg ctgaacttgc tgcgatgatt gcaaaacacg atctggtact
     8221 cgactgtacg gataacgttg cggtgcgtaa tcaactgaac gctggctgtt ttgccgcgaa
     8281 ggtgccgctg gtttccggcg cggcaattcg tatggaaggg caaatcacgg tcttcactta
     8341 tcaggacggc gaaccgtgct atcgctgcct tagccgtttg tttggcgaga acgcattaac
     8401 ctgcgtagaa gcaggcgtga tggcaccgtt gatcggcgta attggttcgt tgcaggcaat
     8461 ggaagcgatc aaactgctgg caggttatgg caaaccggcc agcgggaaaa tcgtcatgta
     8521 cgatgcgatg acctgtcagt ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt
     8581 gtgcgggcag taattgccac ctccaggcca gataagacgc ggcactccgc cacgtcatca
     8641 gcaatttatg aggatgcaga acgtccggtt aaatcgacgt tctgccaaac gctcccagcc
     8701 agtcctgttc aaacttcgcc acagcggctt ccaccgccgg atagctaatc atctgttgtg
     8761 ccacatccag tggcagcgta attgattcac atcctgccag taagcagtcc agcgcctgac
     8821 gcggggtttt gaaactcgct gccagcactt tcgcctgcgg cgcatgcatt ttcaataact
     8881 ggtgtaagtc ggtcacagtc tgaatgccgc taccgccctg agcatcaata cgattaacgt
     8941 aaggcgcaac atattccgca cctgccagcg ccgacagcag cccttgtgct gcgccatata
     9001 ccgcggttcc cagcgttgga atcccttccg cttttaacat cttaatagct gccagcccct
     9061 cggcggtcac cggaactttc accacgatat ccgcaataat agaacgcaat ttacgtgcgt
     9121 cattcaccat cccttcagca gtggtagcca ttacctgggc aaacagacgc ccctgaccgc
     9181 ccatcgcttc atgaagttgc ggaagcacaa catccagcgg ttttttaccc gcggcgataa
     9241 tgcttgggtt agtggtcaca cccgccagcg gaaaaatacg tgacagcgcc ttcaccgcaa
     9301 caacgtctga agtatccaga tacagttcca taaccatcct caaaatgtac tttttgtctg
     9361 aatgtagact atcacgggaa aactctctga acagttgacg cacatcaatc taactttcat
     9421 tcgaaagtat tttaatgttt gaacgaaaca gagaggcaac tatgattttc aatattcagc
     9481 gctactcgac ccatgatggc cccggtatcc gcacggtcgt atttcttaaa ggctgttcgc
     9541 tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg cacgcaggat ctgctgtatg
     9601 acgcacgact gtgtctggaa ggctgcgagc tgtgcgctaa ggccgcgccg gaagtgattg
     9661 agcgcgcgct gaatggtttg cttattcatc gggaaaagtt aaccccggag catctgacgg
     9721 cgttaaccga ctgctgtccg acacaggcat taaccgtgtg tggtgaagtg aaaagcgttg
     9781 aggagatcat gacgaccgtt ctgcgcgata aaccgtttta cgatcgcagc ggcggcggtt
     9841 taacgctttc gggtggtgag ccctttatgc agccggaaat ggcgatggcg ctactgcaag
     9901 ccagccacga ggcaggcatt catactgcgg tagaaacctg tctgcatgtg ccgtggaaat
     9961 atatcgcccc ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa cacgttgccg
    10021 acgcgccgtt taaacagtgg accgacggta acgccgccag agtgctggat aacctgaaaa
    10081 aactcgccgc agcgggcaaa aaaatcatta tccgcgtgcc gctgattcag ggctttaatg
    10141 ccgacgaaac ctctgtaaaa gccattaccg attttgccgc cgacgagctg cacgttggcg
    10201 aaattcattt tctgccctac cacacgctgg gcatcaacaa atatcactta cttaatctgc
    10261 cctatgacgc cccggaaaaa ccgcttgatg cgccagaact gctcgacttt gcccagcagt
    10321 atgcctgcca gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa
    10381 actggacacg ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa
    10441 accgccagtc tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga
    10501 taagccgatc ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat
    10561 ctggatcaaa cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc
    10621 gatcttcccg gaatatactg tctcgtggat cgaaaaagag attgatgatc tggcagatcg
    10681 tcccggtgct ggctttgcgg tgagcgaaga gaacaaacgc gttctgcatg aagtgtgccc
    10741 gtggtggcgc ggtcagaccg tacaggatcg ctgctacggc atgtttaccg atgagcaaaa
    10801 aggtctgctg gcgaccggaa tcattaaagc ggaaggcaat atgacctccg gcgatgcgca
    10861 cctggcggtg aatttcccgc tgctgctgga aaaaggtctt gatggtctgc gtgagaaagt
    10921 agcggaacgt cgctcgcgca tcaatctgac ggtgctggaa gatttacatg gcgaacaatt
    10981 cctgaaagcg attgatatcg tgctggtggc agtcagtgaa cacattgaac gtttcgctgc
    11041 cctggcgcgt gaaatggctg ctaccgaaac tcgcgaaagc cgtcgcgatg aactgctgac
    11101 gatggcagaa aactgcgatc ttatcgccca ccagccgccg cagactttct ggcaggcgct
    11161 gcaactgtgt tacttcatcc aattgatttt gcagatcgaa tctaacggtc actcggtatc
    11221 gtttggtcgt atggaccagt atctctaccc gtactatcgc cgcgacgttg aactcaacca
    11281 gacgctggat cgcgaacacg ccatcgagat gctgcatagc tgctggctga aactgctgga
    11341 agtgaacaag atccgctccg gctcacactc aaaagcctct gcgggaagtc cgctgtatca
    11401 gaacgttacc attggcggac aaaatctggt tgatggtcaa ccaatggatg cagtgaatcc
    11461 cctctcttac gcgatcctcg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag
    11521 cgtgcgttac catgcaggaa tgagtaatga tttcctcgac gcctgcgtac aggtgatccg
    11581 ttgcggcttc gggatgccgg cgttcaacaa cgacgaaatc gtgatcccgg aatttattaa
    11641 actcggtatt gaaccgcagg acgcttacga ctacgcagcg attggttgta tcgaaaccgc
    11701 cgtcggtggc aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt
    11761 gatgctggcg gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca
    11821 agaaaaagcg ttgtcggcag gcaactttaa caatttcgat gaagtaatgg acgcgtggga
    11881 tacgcaaatc cgttattaca cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat
    11941 gctggaagag aacgtacacg atattctctg ctcggcgctg gtggatgact gtattgagcg
    12001 agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggttgg
    12061 cattgccaac ctcggcaaca gcctggcggc agtgaagaaa ctggtatttg aacagggcgc
    12121 gattggtcag caacagctgg ctgccgcact ggcggatgac ttcgatggcc tgactcatga
    12181 gcaactgcgt cagcggctga ttaacggtgc gccgaagtac ggcaacgacg atgataccgt
    12241 cgatacgctg ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa
    12301 cccgcgctac ggtcgtggtc cggttggcgg caactattac gcgggtacct cgtctatctc
    12361 cgctaacgta ccgtttggcg cgcaaactat ggcaacaccg gacgggcgta aagcccacac
    12421 cccgctggca gaaggcgcta gtccggcctc tggtactgac catctcggcc ctactgcggt
    12481 cattggctcg gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt tgctcaacca
    12541 gaaactgaat ccggcaacgc tggagaacga atctgacaag cagaaactga tgatcctgct
    12601 gcgcaccttc tttgaagtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga
    12661 aacgctgctg gaggcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc
    12721 gggctattcc gccttcttca ccgcgctctc tccagacgca caggacgata tcatcgcccg
    12781 tactgaacat atgctgtaat ctcctcggcc cggcggcgtc gtcgccgggc taaatcattc
    12841 acatcatcaa tttcatcctt actttcattc gaaatataat ttgtgctctt cgtcacattg
    12901 ttattacact gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta
    12961 tcgtcacaga catggacggt acttttctta acgacgccaa aacgtacaac caaccacatt
    13021 ttatggcgca atatcaggaa ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta
    13081 atcagtatta ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg
    13141 cggaaaacgg cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc
    13201 gacatgaatc gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg
    13261 cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga
    13321 tggcaaaaca ctaccatcgc ctgaaagctg taaaagatta tcaggagatt gacgacgtac
    13381 tgttcaagtt ttcgctcaac ctgccagatg aacaaatccc gttagtgatc gacaaactgc
    13441 acatagcgct cgatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacctga
    13501 ttattcccgg tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt
    13561 caccgcaaaa tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg
    13621 cgcgttattc ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc gcccgttacg
    13681 ctaccgatga taataatcat gaaggcgcgc tgaatgtgat tcaggcggtg ctggataaca
    13741 catccccttt taacagctga cctctctacc ggagccgtgc gctccggttt tcccttcttt
    13801 ttacatcctg tacatcacat caaatcatta aacttgcatt aacttattta taacttatat
    13861 tgtcacatgc aaatcagaat ggtttcgaat gaaagttaat gagaggtggt tatggcttct
    13921 acttttacca gcgacacatt gcctgccgat cacaaagcag ctatccgtca gatgaagcac
    13981 gcgctgcggg cgcagcttgg cgacgtccag cagatcttta atcagctaag cgatgacatt
    14041 gccacgcgag tggctgaaat caacgcactc aaagcacagg gcgatgccgt ctggccggtg
    14101 ctgtcttatg ccgatatcaa agcaggtcat gttactgcag agcagcgcga acagattaaa
    14161 cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct aggctgggat
    14221 cagtcgatgc tggactatct ggaccgcaac cgctttgacg aggtctacaa aggccccggc
    14281 gataatttct tcgggacgct cagcgcttca cgtcccgaga tttaccccat ctactggtcg
    14341 caggcgcaaa tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat
    14401 cgtctgtgga catttgaaag tgatggaaag caatggttta acccggatgt gagcgtcatc
    14461 taccctgacc gtatccgccg ccgtccgccc ggaacgacct ccaaaggtct tggagcgcat
    14521 accgactccg gggcgctgga acgctggctg cttccagcgt atcagcgcgt tttcgccaac
    14581 gtctttaatg gcaatctggc gcaatatgat ccctggcatg cggcacatcg tacggaagtt
    14641 gaagagtaca cggtggacaa caccaccaaa tgttccgtgt ttcggacatt ccagggctgg
    14701 acagcgctct ctgatatgct gcctggtcag gggttgctgc acgttgtgcc cattcctgaa
    14761 gccatggcgt acgtactgtt acgtccgctg cttgatgatg tgccggagga tgaactgtgc
    14821 ggcgtagcgc ccggaagagt gttgccggta tcagagcaat ggcatccact gttaattgag
    14881 gcgttaacca gcattccaaa actcgaggcc ggagactccg tctggtggca ctgcgacgtc
    14941 atccattccg ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat gtacattcct
    15001 gcggcaccga tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa
    15061 aaaggcgcat cgccgggcga cttcccgcgc gaggactatg aaacaaactg ggaaggacgc
    15121 tttacgcttg ccgacctcaa cattcacggt aagcgagcgt tgggcatgga tgtttgagag
    15181 ttaatggaag tgaattgatg tgggattaag aaaatcgatt cgacgttact cagattgctg
    15241 acaagtgcgc tttcttaatg cctgatgcgc tacgcttatc tggcctacaa agtcctgcag
    15301 attcaatata ttacaggtac t
//
LOCUS       DAZFWP020000117        15234 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFWP020000117 DAZFWP020000000
VERSION     DAZFWP020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.117"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            51..617
                     /gene="yceJ"
                     /locus_tag="ACHK0Z_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             51..617
                     /gene="yceJ"
                     /locus_tag="ACHK0Z_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4503498.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            621..1196
                     /gene="yceI"
                     /locus_tag="ACHK0Z_RS17700"
                     /old_locus_tag="pgaptmp_003540"
     CDS             621..1196
                     /gene="yceI"
                     /locus_tag="ACHK0Z_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU4503499.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1238..2290)
                     /gene="trhO"
                     /locus_tag="ACHK0Z_RS17705"
                     /old_locus_tag="pgaptmp_003541"
     CDS             complement(1238..2290)
                     /gene="trhO"
                     /locus_tag="ACHK0Z_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU4503500.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2515..3435
                     /gene="lpxL"
                     /locus_tag="ACHK0Z_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             2515..3435
                     /gene="lpxL"
                     /locus_tag="ACHK0Z_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU4503501.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3607..4833
                     /gene="mdtG"
                     /locus_tag="ACHK0Z_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     CDS             3607..4833
                     /gene="mdtG"
                     /locus_tag="ACHK0Z_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGU4503502.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4916..5290
                     /gene="msyB"
                     /locus_tag="ACHK0Z_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             4916..5290
                     /gene="msyB"
                     /locus_tag="ACHK0Z_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4503503.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5291..5518)
                     /gene="yceK"
                     /locus_tag="ACHK0Z_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             complement(5291..5518)
                     /gene="yceK"
                     /locus_tag="ACHK0Z_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4503504.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5691..8234)
                     /gene="mdoH"
                     /locus_tag="ACHK0Z_RS17730"
                     /old_locus_tag="pgaptmp_003546"
     CDS             complement(5691..8234)
                     /gene="mdoH"
                     /locus_tag="ACHK0Z_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU4503505.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8227..9762)
                     /gene="mdoG"
                     /locus_tag="ACHK0Z_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             complement(8227..9762)
                     /gene="mdoG"
                     /locus_tag="ACHK0Z_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU4503506.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10156..11313
                     /gene="mdoC"
                     /locus_tag="ACHK0Z_RS17740"
                     /old_locus_tag="pgaptmp_003548"
     CDS             10156..11313
                     /gene="mdoC"
                     /locus_tag="ACHK0Z_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU4503507.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11321..12802)
                     /gene="clsC"
                     /locus_tag="ACHK0Z_RS17745"
                     /old_locus_tag="pgaptmp_003549"
     CDS             complement(11321..12802)
                     /gene="clsC"
                     /locus_tag="ACHK0Z_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU4503508.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12744..13277)
                     /gene="ymdB"
                     /locus_tag="ACHK0Z_RS17750"
                     /old_locus_tag="pgaptmp_003550"
     CDS             complement(12744..13277)
                     /gene="ymdB"
                     /locus_tag="ACHK0Z_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU4503509.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13372..13683)
                     /gene="ymdA"
                     /locus_tag="ACHK0Z_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             complement(13372..13683)
                     /gene="ymdA"
                     /locus_tag="ACHK0Z_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU4503510.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13804..14136)
                     /gene="csgC"
                     /locus_tag="ACHK0Z_RS17760"
                     /old_locus_tag="pgaptmp_003552"
     CDS             complement(13804..14136)
                     /gene="csgC"
                     /locus_tag="ACHK0Z_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU4503511.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14195..14650)
                     /gene="csgA"
                     /locus_tag="ACHK0Z_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             complement(14195..14650)
                     /gene="csgA"
                     /locus_tag="ACHK0Z_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU4503512.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14691..15146)
                     /gene="csgB"
                     /locus_tag="ACHK0Z_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             complement(14691..15146)
                     /gene="csgB"
                     /locus_tag="ACHK0Z_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU4503513.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3772 a         3923 c         3649 g         3890 t
ORIGIN      
        1 tctccgacac gccattttct ttctctcgca aattcgcatt ggatgcactt atgtcattca
       61 caaatacccc tgaacgctat ggtgttatat ctgcggcctt ccattggcta agcgcaatta
      121 ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat tacgatggct
      181 ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat tttgttaatg atggggctgg
      241 ttattcgcgt tctgtggcgt gtcatatctc caccgccggg tccgctgccg agttattcgc
      301 ccatgactcg tcttgccgca aaggctggac atctcgcatt gtacctattg ctgttcgcca
      361 tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc gtttttggct
      421 ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca ggcagacttt gccggtgccc
      481 tgcatttttg gctcgcgtgg agcgtcgtag tactgtccgt tatgcacgga tttatggccc
      541 tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga aagtcgtcat
      601 ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga
      661 tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa ggtcagcacg
      721 cctttgttaa tttccgcatc cagcaccttg gctatagctg gttatacggc acctttaaag
      781 atttcgacgg tacttttacc tttgacgaaa aaaatccggc tgccgataaa gtgaatgtga
      841 caattaacac caccagcgtc gatactaatc acgccgaacg cgataaacat cttcgcagtg
      901 cagatttcct caataccgca aaatatccac aggcaacatt cacctccacc agcgtgaaga
      961 aagacggtga cgaactggat attaccggcg atctgacgct gaatggcgta accaaacccg
     1021 tcacgctgga agcgaaatta attggtcagg gcgacgaccc atggggtggt aaacgtgcag
     1081 gcttcgaggc cgaaggcaaa attaagctca aggacttcaa tatcaagaca gatttaggtc
     1141 cagcttctca ggaagtggat ctgattattt cagtggaagg cgtacagcag aagtaagagt
     1201 gaaatgttgc cggatggcac gcatccggca atgatattta ttccgttgga tcaggaatgc
     1261 ccagtgttgt attcagacgt ccacgagact tattaaagat cttattgcca ttttcacgtc
     1321 ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag caaatctcac
     1381 tacaacaacc tttgtatttt tccgcacata ctggacactg aataaacagc aggtggcagc
     1441 catcattttt acagttggta tggctgtcgc acggcgcgcc gcactggtgg caatgcgcga
     1501 taatctcatc cgatatacgt tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa
     1561 aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga
     1621 tatgccagac tttgttgaat ccgttatgtt tcatccaggc actggccttt tcacaacgaa
     1681 tgccgccggt gcagtacatg acgatttttt tatctttatg tgcctgcatc atctcgactg
     1741 cttttggcag ctgctcacgg aaggtatctg ccggaatttc gagcgcgttt tcaaagtgcc
     1801 ccacttcata ttcatagtgg ttacgcatgt cgataaatag tgcatcggga tcgtcaagca
     1861 tggcgttcac ttccgccgct tgcagatact caccaacatt gctggcatca aagtgaggat
     1921 cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat gcgcagtacc cagaaggatt
     1981 tcccgtcatc atccaacgcg atattcaggc gtaaaccctc taaagccggg tcgaaggcat
     2041 agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc
     2101 cctcatgcgc cagatacact cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag
     2161 cgtcacgggt cgccttagga tcggcgatgt ggaaatactt gtaaaacgaa atggtggttc
     2221 gcggttcgct ctcagccaac attttggctt ttagcgcgtc gttggaaatg cggttgtgta
     2281 acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg gcatgatata
     2341 gcaattatcg ataattaaca tccacacatt ttacgctaca tttgcgcatt aaaaattatt
     2401 tgttatttac aagcgcggca atttcgccca gtcttcagcc acaaattttg gttgcgggcg
     2461 aaaaaatgcg acaatacata caattgcccg aataggttga aaaacaggat tgatatgacg
     2521 aatctaccca agttctccac tgcactgctt catccgcgtt attggttaac ctggttgggt
     2581 attggcgtac tttggttagt cgtgcaattg ccctacccgg ttatctaccg cctcggttgt
     2641 ggattaggaa aactggcgtt acgttttatg aaacgacgcg caaaaattgt gcatcgcaac
     2701 ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat
     2761 ttcgaatccg ttggcatggg cctgatggaa accggcatgg cgtggttctg gccggaccgc
     2821 cgaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa
     2881 aaacgcggca tcctgttagt tggcatccat tttctgacac tggagctggg tgcgcggcag
     2941 tttggtatgc aggaaccggg gattggcgtt taccgcccga acgataatcc actgattgac
     3001 tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg caaagattta
     3061 aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat
     3121 tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg ttgagcaggc tgcgaccacg
     3181 accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc
     3241 cgtaagccag atggcaaagg gtatcaattg attatgctgc cgccagagtg ttctccgcca
     3301 ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg
     3361 atggcaccag agcagtatat gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt
     3421 ccttcacgct attaaatctc ccctgccgga tgcttcagaa tggcatccgg cattaccaca
     3481 gcaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg aagtcgggcg
     3541 cataattagt gtgcttatct tttcttctta tgttcaccgc gcctggcgca ccaacagcgg
     3601 attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa cctgatcgtc
     3661 gcctggctag gctgttttct taccggtgcc gccttcagtc tggtaatgcc cttcttaccc
     3721 ctctacgttg agcagcttgg cgttaccggt cactccgccc tgaatatgtg gtccggtatt
     3781 gtcttcagca ttacattttt attttcggcc atcgcctcac cgttttgggg tggactcgcc
     3841 gaccgtaaag gccgaaaact catgctatta cgctctgccc tcggcatggg catcgtgatg
     3901 gtgttgatgg gactggcaca aaatatctgg cagtttttga tcctacgggc gcttcttggg
     3961 ttacttggcg gatttgtccc caacgctaat gctcttatcg ccacacaagt accgcgtaat
     4021 aaaagcggct gggcgctggg tacgctctcc acaggcggcg ttagtggtgc gttgctcggc
     4081 ccaatggctg gcggcctgct cgccgatagc tacggcttac gtccggtatt ctttattacc
     4141 gccagtgtgc tcatactctg ctttttcgtc accctgttct gcatcagaga aaaattccag
     4201 ccggtcagca aaaaagagat gctgcacatg cgggaagtgg tgacatcact taaaaacccg
     4261 aaactggtac tcagcctgtt tgtcaccacg ttaatcatcc aggtggcgac aggctcaatt
     4321 gcccccattc tgacgctgta tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt
     4381 atcagtggca tgatcgcctc ggtgccaggc gtggcggctc tgctaagcgc accacgactc
     4441 ggcaaacttg gcgatcgaat cggacccgaa aagatcctga ttacagcgct gatcttttct
     4501 gtactgctgt tgatcccaat gtcttacgtt cagacgccat tgcaacttgg gattttacgt
     4561 tttttgctcg gtgccgccga tggtgcacta ctccccgccg tacagacact gttggtttac
     4621 aactcgagca accagatcgc cgggcgtatc ttcagctata accaatcgtt tcgtgatatt
     4681 ggcaacgtta ccggaccatt gatgggagca gcgatttcag cgaactacgg tttcagagcg
     4741 gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag tctattcatg gaacagtcta
     4801 cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc
     4861 ggagaatcag ctatcctttt ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac
     4921 catgtacgca acgcttgaag aagccattga cgctgcacgc gaagaatttc ttgcagacaa
     4981 ccccggcatc gacgccgaag atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt
     5041 gcaggacggc gacatcatgt ggcaagttga gttttttgcc gacgaagggg aagaaggtga
     5101 atgtttacct atgcttagcg gtgaagccgc gcaaagtgtt tttgatggcg actatgatga
     5161 gatagagata cgccaggagt ggcaggaaga gaatacatta catgaatggg acgaggggga
     5221 atttcagctt gagccaccgc tggataccga ggaaggacgc gcagcagctg atgagtggga
     5281 tgaacgttaa tcactcatac gggccatgat gaatgtcgat cggcagcagt aaagtatcaa
     5341 aaaccagaga gaatggcaga tcaaggatcg tgacataacg ccaggcggag tcacgcacat
     5401 cccattgcac accgggataa tattggttgc catgcccctg ccccggtata gtgcgactaa
     5461 taatgctgcc acagccactc agcaaggtca ccatgatgct caccacaatt aaacgcattt
     5521 gatacctctt tcgtaaaaaa ataccggcat aacgccggta tttttattgg ctcgttgttt
     5581 ttgccggatg cggcgtgaac gccttatccg acccacaaaa tcttgcaaaa tcaataaatt
     5641 gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc aactacgttt ttattgcgaa
     5701 gccgcatccg gtttacgcaa tgccagtgga ttgagcttta tcccttcgta ataactcacc
     5761 catgaagaat atctctccgg ggaattccag acgcggaaat gcagacgggc catcgtcacc
     5821 ggatcgctta gcagcaccag gcgacgatcg cgattcagct tctctggcgt ctcgttcagc
     5881 gcctgttcaa cgtggcggtc acgggcgatt tccagcacct tactggcgcg gtgacgcgcg
     5941 gtcgccattg cggttgccag agcgttaaat gacgggttaa acactgcgtg cataaagcca
     6001 tcatcaaggg agcgttgacg attcatctca aggaaccgat cggtatcaac cagcacctgc
     6061 ggcggcgaat actcttccgg gatcaggaac agtttccagc gtttggtgcg cagaccaacg
     6121 gtggcacggc tggaaatcac cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt
     6181 gccagccaga acaggaaacg cagatccaac cacgccatac caacagccca cactaaccct
     6241 aacagcagct gtgagccgtg gcgtttgaac gcttcacccc aggaagtgga gtcatcatca
     6301 cgctgcggtg aattccacac cacttcccag ccaaggaacg cgctgacgac gaagaccgta
     6361 tggaacagca tgcgtaccgg agccagcagc acggaaaaca gcacttccag cagcaacgat
     6421 aatgtaacgc gccagaagcc gccgtattct ttcgttcctt tgcaccagat aagcaaaatg
     6481 ctcaataact tcggcaggaa caacagcacc atggtcgaag caaaaagtgc aatcgccagc
     6541 tcaggacgcc actgcggcca caccgggaac aactgccgtg gttgcaggaa gtattgcggt
     6601 tcggtcaacg catgtactac ctgcaatgca gtagagagcg cgaggaacat aaaccacagc
     6661 ggagcggaga gataagacat cacgcccgtc aggaataccg cacggtgaac cgggtgcata
     6721 cccttcacca ggaacagacg gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt
     6781 tttagctcat caagcaagtt aggcggcaat tcttcataag aacccgggag atcgtaagca
     6841 atccagaccc cccaacctgc acggcgcatc aacgccgctt ccacgaagtc atgtgacagg
     6901 attgaaccgg caaaggaacc ttcgcccggc agcggagcca gtgcgcagtg ctcgataaac
     6961 ggtttcacgc ggataatcgc gttatgtccc cagtagtgcg actcgccaag ttgccagaag
     7021 tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg cgaactgctg acagcgcgca
     7081 tacagcgtat ccataccgga cgctttcggc gacgactgaa tgatcccggc gttcgggttg
     7141 gcttccatca ggcgcaccag gccgcataaa caatcaccgg tcattaccga gtcagcatcc
     7201 agcaccacca tgtagctgta ctggctgccc cagcgacggc agaagtcatc gatattaccg
     7261 cttttacgct tcacgcggcg acggcggcgg cgatagaaaa tctgaccttc gccaccgact
     7321 tcagcgataa gctccatcca ggctttttgc tctgcgacgc agatatccgg gttataactg
     7381 tcactaagaa tgtagacatc aaagtgtttg gcattcccgg tggcttttac tgattcccac
     7441 gttgcacgca ggccagcaaa aacacggttc acgtcttcgt tacagatagg catgatcaac
     7501 gccgtgcgat gctccgggtt taatggttca tcgccaactg ttgacgcaga tatactgtat
     7561 ttatcgcgac caataagcag ttgcaggaag cccattaacg ccgtccagaa tccggcggac
     7621 acccaacaga acagtaccgc aaagaggatc aggataccgg tttgcagcat ataaggcaga
     7681 agctgcataa aggaaaccca caaatcctga ccaaccatat ccataggatt aatcagcgcc
     7741 caaccctgat aaggaagaat ggtcttcata taccaggtcg cgacgacagt ttgcgcgagc
     7801 gtcaggatca acagaatgta acggcggatg gtaccgacgg tacgccactt ttgctcgctc
     7861 tcctgctctt ctttggtcaa acgagccaga tagcgcggcg tgacatcgcg tccacgcagg
     7921 cgatcccaga aacggcctac cgggttggta cgccacgggt cgggaaacat cgaggagcgt
     7981 tttgcttctg gcatcgcctt cagctgatcg cgcccttcgt cgtctttaat taactgtcca
     8041 tcagcaagtg aatctggcca ggcttgttcc agacgcgcct ttaccgagcc ttgcggggaa
     8101 tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg cctgatgaac ggcgcggata
     8161 tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg gcattgcgtc aatgtactca
     8221 gttgtcttat tcattggcag gtaactggta gctccaggtt tcactcaacg tctgatcggc
     8281 attcaccagc gcagcacgca tttcagtggt tttcttggca tctttcactt tcacacgcat
     8341 caccagacgc cagcctttgg taaccgggtt atagcgcacc gtgctttcaa ctatctcacc
     8401 attatcacca atgctggttt gcgctgtgac cggggtatcc tctggcagtt ttttcatctc
     8461 tgcgccggta aaatcgacca caaaggcgat agtaccgtca ggctggcgaa tcaggttcga
     8521 ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg
     8581 cagtttgtct tcatcacggc tgaaggtgat ggtgtattta aagttcatct ctttacccgg
     8641 ctccggcagc tgatccggcg tccagtaagc gacgatgtta tcgttggttt catcgttggt
     8701 tggaatttct accagctcaa cgctgccttt accccactcc cctttcggag tcacccatgc
     8761 gcttggacga agatcgtaac gatcatcgag atcttcaaag cgggagaaat cacgaccgcg
     8821 ctgcaacaga ccgaagcctt gcgggttttc catagagaag ctgctgaccg ctaaatgttt
     8881 cgggttattc aacggacgcc agatccattc gccattaccg gcatggatag agagaccgtt
     8941 agagtcgtgc aactccggac gatagttatt tgcaggcgac ggttggttcg gcccaaacag
     9001 gaacatactg gttaacggtg caacccccag tttgccgact ttatcgcgca gatagatttt
     9061 cgactgcaca tccacaaccg tgtcacgccc tggcataact acgaatttgt aagcacctgt
     9121 cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt ttatcagtcg gttttggacg
     9181 ctcgatccag aactctttga agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat
     9241 tgccaggccg cgggcagaaa ggccataaac ctgacctgca ccaatcacgc ggaaatagct
     9301 ggccccgagc atgctgacga tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac
     9361 tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa
     9421 agtgaaataa tccgggctgt atttgattcg tttgactgcg gtggcagtca cttcatttat
     9481 tttgaccggg gtatcgaagt acataccctg atggtagaac tcgagtttga atggggtctt
     9541 cagattgttc cagtacgctt tgtcatgatt aaactggatc tgctgatagt ccgcgtattt
     9601 catatcgcgg aaaacggagg gcaagttgct tttgggcgcc tcatagcctt tcccggctaa
     9661 ggattgagct tgctttgcga catcatcaat actgaaagcc cagcttgaag atgtatacag
     9721 ggttaacatt actgcagcac tcaaccaacg cattttcatc atttgtagtt tatgtttcat
     9781 aattagtaag cacttccccc tttgtgtgct tatatcgatc cgatctattt taatggataa
     9841 ccagccattc cgacaacaga atcccggtat cgttcagcac gctggctcat ggattaagca
     9901 cctgattgca accgttttca ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt
     9961 gtagggtttg ccagcaattt tatttatcaa ccgtcttaca gacgcttcca ttctggttag
    10021 tggtagcgat gatgctatgc ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg
    10081 tgtcgctatg gcaacaagcc taaaggcata acccgacagg gataagacga aaaatccgag
    10141 attagttaac catatatgaa tccagtaccc gcgcaacgtg aatatttcct cgactccatc
    10201 cgcgcctggc tgatgttgtt agggatccct tttcatattt ctttaatcta ttcgagccat
    10261 acatggcatg tgaatagcgc cgaaccgtca ttgtggctga ccctttttaa tgacttcatc
    10321 cactcgttcc gcatgcaggt atttttcgtt atatccggct acttttccta catgcttttt
    10381 ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg
    10441 ttaacagcca tccccttact gacattgccg caatttatta tgctgcaata cgtcaaaggg
    10501 aaagcggaaa gttggcctgg gctgtcattg tatgacaaat ataatacgtt ggcctgggaa
    10561 ttaatatcac atctgtggtt tttactggtg ttagtggtca tgacgacgct gtgcgtatgg
    10621 atatttaaac gcatcagaaa taatttagaa aattctgata aaacgaataa aaaattctcg
    10681 atggtaaaac tatcggtgat ttttttatgc ctcggcatcg gttatgcggt aataagaaga
    10741 acgattttta ttgtgtatcc gcccattctg agtaatggca tgttcaattt tattgtcatg
    10801 caaacgctgt tttatttgcc gttctttatc ctcggcgcac tggctttcat tttccctcat
    10861 cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc ttgcagcagc attggcgttt
    10921 gtcgcttatt tactcaacca gcgctatggc agtggcgatg cctggatgta cgaaaccgag
    10981 tcggtgatca ccatggtcct cggtctgtgg atggtgaatg tggtcttctc ctttggccac
    11041 cgtttgctta acttccagtc agcgcgggtg acttattttg ttaacgcatc gctgtttatc
    11101 tatctggttc accacccgtt aacgctgttt ttcggcgcat acattacacc gcacatcacc
    11161 tccaactggc ttggttttct ctgtggcctg atatttgtag tagggattgc gataattctg
    11221 tatgaaattc atttgcgcat cccgttactg aagtttttgt tctctggtaa accggttgtt
    11281 aagcgtgaga acgataaagc accagcccgt taagccacat ttacaataac cattccacgg
    11341 gcaatatcga cgccagtctg accataaccc gcttccagaa actggtggcg ggttcttttt
    11401 tgagaataat ctctttcttt gcatgacgat caacccagtt gatccgtccc cacctgtcca
    11461 gacggagctg ccaggccgca tcatactggc tctgaataaa gcgtttatca attaactgtg
    11521 ccagcgtttc gctctctatc acgaagccca tttcagtatt gagcaatgtt gaacgcggat
    11581 cgaaattgaa agagccgata aacaccgttt taccatcgat gctaaaggtt ttagcatgca
    11641 ggctggctcc ggaattaccg gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg
    11701 tcggcttgag ttcatataat tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt
    11761 atccggcatg gacgacagca acatcgttag cggcaagaga attggttagg atcgcaatct
    11821 ttaccccttt tctcaccatc cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat
    11881 aggaagagat aatatcgatg cgttcactgg gtgagcccat gatatcgaac aggcgctgcg
    11941 gtaacagtga atgccgtttt gccttgccct cccctttcgc cggatcatca cttaataaac
    12001 gtgttttcgc ccagataagc ggcaacgttc catcaaccag atgatttata aacggactgg
    12061 attccatttt gcgtaagtaa cgatgcgtca tagcatcgtt atgccaggag gcgggtaact
    12121 cgatgcgatc cgccatttca ccttccggga catccagcac ttgctgtaag ggtgaaaccg
    12181 atttgcaata ccagtagcgg gcgaaatcat cggcaacgtc ctctaccacg ggccctattg
    12241 ccataacatc taaatccgaa aaaagtggct cttcccctgc cccaaaatag gcatcaccaa
    12301 tatttcgccc ccctaccagc gtcaccacgc catcgacagt gaaactttta ttgtgcatac
    12361 ggcgattgag acgggaaaag tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga
    12421 aaggattaaa aagccggact tcaatgcggg gatggctgtc aagcaagcgt aaaatgtcgt
    12481 caagtccggg cgtattgttg tcgtccagca acaaacggac gcgaacgcca cgctttgctg
    12541 cggctaacag ggcggaaaac agtaaccgac ccgacatatc gtcctgccag atgtaatact
    12601 gaacatccag cgtatgctct gccatttcgg ccagacgata ccgggcggca aacgcatcca
    12661 gacttttctc cagagggaaa agaccacact gaccgggatg ttgcgaacac agtggcagca
    12721 ccgcgctcgc cagccggggc aaatcattca tctccttgtt gggtaaggag tctttcgtag
    12781 aggtgggcgt tttcttcatc ataacagaca aagtatacct gttcgggtaa agcgtgacgg
    12841 gtaataaatt ctgaaacggt tttaactgcg atttcagccg ctgccgcacg agggtaacca
    12901 taaaccccag tactgattgc aggaaaagcc actgacgtat agctgtttgc cgccaccagt
    12961 cgtaggctat tgagataggc atcctgcaaa agctggtctt cgttttgttc accaccacgc
    13021 cagactggcc cgacggtgtg cactacggct ttagcgggaa gagcgcctgc aagcgtaata
    13081 acggcatgcc ccgtagggca atcgccctgc tgttgcctga cttttaaaca ggcatccagc
    13141 agggccggac ccgctgcgcg atgaatggcc ccatcgacgc cgccgcctcc cattaatgac
    13201 ggattagccg cattcacaat cacatcaacg gccagtttgg taatatcacc ctgcacaaca
    13261 tgaatacgcg ttttcataac gcctccttac accttcttgt ttcttaagtg tacagcaggc
    13321 tttttggtta aatcgcattt ttctgctcag caacgtgcgt tgttcagaaa cttagttata
    13381 ttcgatgttc attacagcca gctttttctg tttatcaaga tactgcactt ttactgaggc
    13441 tatttgcgcg tttttaacat taccgctcat taacgctttg ctggaatagg ttttgcccgg
    13501 aacagaacca ttctgtgggc agttcatttc tacgggtgat gactgggtgc tgacgtcaca
    13561 tgccggttcc acaatttggc cataaaaatg gatcaccccc ccttgcgcgg tgcttcctgc
    13621 gatggcaata aaaggaaaaa gcaaactgcc gagcataaga gagtttaaaa aaggtctgaa
    13681 catttattct atttcctcaa tgattagtca tccttgaggg ttgtgttatc catactttca
    13741 ggttggctga ttcgcagcag accattctct ccagattcat cttatgctcg atatttcaac
    13801 aaattaagac ttttctgaag agggcggcca ttgttgtgat aaatgaagtg actgtccatc
    13861 agaaacagta acaactattt tcacccgatc ttccggggaa atatttaaac tcaacttcgt
    13921 caaagcaata ggttgattag caggcaatga gagggtcttt tcttgcttcg tctgactttg
    13981 ccctgaactg ccttcgcgca gggacaatat ttgtactctg cacagacaag attgagtaag
    14041 agtgacttca ggaataatgg tatacacatc cccttgctgg gtcgtattaa aggttatctg
    14101 gctggaaagt gccgcaagga gtaataacgc attcatattc ttctcccgaa aaaaaacagg
    14161 gcttgcgccc tgtttctgta atacaaatga tgtattagta ctgatgagcg gtcgcgttgt
    14221 taccaaagcc aacctgagtc acgttgacgg aggagttaga tgcagtctgg tcaactgcag
    14281 caccgttgcc accaccgaac tgtttaacag tcatttcaga atttttgccg ttccactgat
    14341 caagagtggc gctgttaccg aagccacgtt gggtcagatc gattgagctg tcatctgagc
    14401 cctgacctac atctgcacca ttaccaccgc catgctgggt aatagtcaag tcagagttac
    14461 gggcatcagt ttgcagagca agtgcagagt taccgccacc gtactggtaa atgttcagct
    14521 ctgaatttgg gccgctatta ttaccgccac caccgtggtt accgccgccg ccgtactgag
    14581 gaacaacacc tgccagagcg ctaccggaga atacgattgc tgcaattgct gctactttta
    14641 aaagtttcat gtaaaacccc catcggattg atttaaaagt cgaatggaaa ttaacgttgt
    14701 gtcacgcgaa tagccatttg cgactgtctc tgcactacaa ttgccgtttt ttgagtacca
    14761 tactgtgtaa tatttgcttt attaccagaa cctttctgga taatcatcgc agtattacca
    14821 taagcacctt gcgaaatact ggcatcgttg gcactgcccg cctgatcaat atatgcaagg
    14881 ttataatctc ctgtctggtc aatctttgcc cggttgctac taccttcttg cgcaacaacc
    14941 gccaaaagtt ttgagcctcc ctgccgtaac tgagcactat tattcgtccc agcttgacca
    15001 attatggctg cctgattaaa tgaagactta ctcaattcat ttaccgcgaa gttatattct
    15061 gaattagcta aatcataacc tgctgcggct gcaatcccag gcgcacccag tattgttaac
    15121 atcataaata acaatttgtt tttcatgttg tcaccctgga cctggtcgta catttaagaa
    15181 attaaatcat ttcaacttgg ttgttaacgc aacctgtatt ttgttaacgc tgcg
//
LOCUS       DAZFWP020000118        15052 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFWP020000118 DAZFWP020000000
VERSION     DAZFWP020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.118"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(86..2365)
                     /gene="maeB"
                     /locus_tag="ACHK0Z_RS17775"
                     /old_locus_tag="pgaptmp_003555"
     CDS             complement(86..2365)
                     /gene="maeB"
                     /locus_tag="ACHK0Z_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU4503514.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2654..3604
                     /gene="tal"
                     /locus_tag="ACHK0Z_RS17780"
                     /old_locus_tag="pgaptmp_003556"
     CDS             2654..3604
                     /gene="tal"
                     /locus_tag="ACHK0Z_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4503515.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3624..5627
                     /gene="tkt"
                     /locus_tag="ACHK0Z_RS17785"
                     /old_locus_tag="pgaptmp_003557"
     CDS             3624..5627
                     /gene="tkt"
                     /locus_tag="ACHK0Z_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4503516.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5723..6766)
                     /gene="ypfG"
                     /locus_tag="ACHK0Z_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             complement(5723..6766)
                     /gene="ypfG"
                     /locus_tag="ACHK0Z_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU4503517.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6892..7467)
                     /gene="nudK"
                     /locus_tag="ACHK0Z_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             complement(6892..7467)
                     /gene="nudK"
                     /locus_tag="ACHK0Z_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU4503518.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7535..9514)
                     /gene="aegA"
                     /locus_tag="ACHK0Z_RS17800"
                     /old_locus_tag="pgaptmp_003560"
     CDS             complement(7535..9514)
                     /gene="aegA"
                     /locus_tag="ACHK0Z_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU4503519.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9720..11420
                     /gene="narQ"
                     /locus_tag="ACHK0Z_RS17805"
                     /old_locus_tag="pgaptmp_003561"
     CDS             9720..11420
                     /gene="narQ"
                     /locus_tag="ACHK0Z_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU4503520.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11584..14697
                     /gene="acrD"
                     /locus_tag="ACHK0Z_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             11584..14697
                     /gene="acrD"
                     /locus_tag="ACHK0Z_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU4503521.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14796..14855)
                     /gene="ypfM"
                     /locus_tag="ACHK0Z_RS17815"
                     /old_locus_tag="pgaptmp_003563"
     CDS             complement(14796..14855)
                     /gene="ypfM"
                     /locus_tag="ACHK0Z_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU4503522.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3539 a         3985 c         3921 g         3607 t
ORIGIN      
        1 aaactcagta ataagcttta ctaatatatt gcagccaaac cgcttaccct gaatattcag
       61 ggtaagcgtg agagttaaaa aagaattaca gcggttgggt ttgcgcttca accacggcca
      121 gcgccaccat gttgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
      181 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
      241 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
      301 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt
      361 gcatttcacc atcaatcatc agatctggcg cacgatccct gaccagttcc agcgcctgac
      421 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
      481 cgcgcggctc aataccaaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
      541 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg
      601 gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
      661 ttttcaccac gctaaaatgt tcatgataat cgcccaccgt accgcagatc attgcatcgg
      721 cttccccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcccgct
      781 gcgcctgttc ctgagtgaca ccgcgacgct tcatgatctg gaagtattcg gtccagtact
      841 ctttaaagcg cggatcggat tcgttattga cgatctcaaa atcaacgccc gctttgatct
      901 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
      961 tcgccagtcc cagcgttacc agttcctgag tggcatgcag aacgcgcgcc tcttcccctt
     1021 ctggcagaac aacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
     1081 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
     1141 gacgcgtcgc cacgcccgac tccatcgcgg ctttagcgac cgcaggagcg atcttaacga
     1201 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgat
     1261 cgccatacgc tgaagccacc acttcgctct gttcggcgtg ggccagttcc gcaatcgcgc
     1321 gtaccgccgc cagtttcatc tcttcattga tagccgttgc gccaacatcc agcgcgccac
     1381 ggaagataaa cgggaagcaa aggacgttgt tgacctggtt tggatagtca gaacgaccgg
     1441 tgcaaatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
     1501 ggttcgccag cgccaggatc attggcgcac gagccatttt cttcaccatt tcctgggtca
     1561 gcactttcgg gccggaacat ccaaggaaaa tatccgcgcc ttcaatcaca tcatcgaggg
     1621 tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccatg tttggctcac
     1681 ggccctgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagaccca
     1741 gcgctaccag caggttcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
     1801 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
     1861 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
     1921 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatt ccgccgaagg
     1981 tcggttcgag cgcagcgaca acttcaataa atttgtccgg gtcgagttca tcaacttcaa
     2041 tgtcaaatac atcaatcccg gcgaatttct taaacagaac gcccttgcct tccatcaccg
     2101 gtttgccagc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
     2161 ccgccaccag gttaccgcgc gcggtatatt tgtaggcttt taacgggtct ttttcgattt
     2221 caagacaagg tgcggcaacg cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca
     2281 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
     2341 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
     2401 gtgtgtgggc aacagcccct gcccacaacg tggcgcacat tattaccctg ccggagtcta
     2461 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata
     2521 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
     2581 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
     2641 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
     2701 cagcggcgat attgagtcca ttcgccatta tcatccccag gatgccacca ccaatccttc
     2761 gctgttactc aaagctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
     2821 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
     2881 ggcagtcaat ttcggtgctg aaattctcaa aatcgtaccc ggtcgcgtgt caacagaagt
     2941 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
     3001 cttgtatcag caacaaggcg ttgagaaatc acgcattttg atcaaactgg cttcgacctg
     3061 ggaaggaatt cgcgcggccg aagagctgga aaaagaaggt attaactgca acctgacgct
     3121 gctgttttct tttgcccagg cgcgggcctg tgcagaagcg ggcgtttttc tgatttcgcc
     3181 gtttgtcggg cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt
     3241 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
     3301 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
     3361 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
     3421 gccagtggta cgtaaattaa tcccaccttc tcagacgttc ccacgcccag ctcccatgag
     3481 cgaagcggag ttccgttggg agcacaatca ggatgcgatg gcggtagaaa aactgtctga
     3541 aggcattcgt ctgttcgctg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
     3601 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
     3661 tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg
     3721 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taatcctacc gacccaacct
     3781 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
     3841 tgctacatct gaccggttac gacctgccgc tggaagaact gaagaacttc cgtcagttgc
     3901 attcgaaaac cccaggccac ccggagattg gctatacgcc aggtgttgaa accaccaccg
     3961 gtccgcttgg acaaggtttg gcgaacgccg tcggactggc gatagcagag cgtacactgg
     4021 cggcgcagtt taatcagccg gatcatgaaa ttgtcgatca cttcacctat gtgtttatgg
     4081 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggcg ggcacgctgg
     4141 gactgggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag
     4201 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
     4261 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
     4321 tgaaagataa gccgtcgctg attatctgcc gtacggtgat tggctttggt tcgccgaata
     4381 aagcaggtaa ggaagaggcg cacggcgcac ctctggggga agaagaagtg gcgctggcac
     4441 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagagatt tatcacgcct
     4501 gggatgcccg cgaaaaaggc gaaaaagcgc agcagagctg gaatgagaag tttgccgcct
     4561 ataaaaaggc tcatccgcaa ctggcagaag agtttacccg tcggatgagc ggtggtttac
     4621 cgaaggactg ggagaaaacg actcagaaat atatcaatga gttgcaggcg aatccggcga
     4681 aaatcgccac ccgtaaggct tcgcaaaata cgcttaacgc ttacggaccg atgctaccgg
     4741 agctgctcgg aggttcagcg gatctggctc ccagcaacct caccatctgg aaaggctctg
     4801 tgtcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca
     4861 tgaccgctat cgccaacggc attgcgcacc acggcggctt tgtgccgtat accgcgacgt
     4921 tcctgatgtt tgttgaatac gcccgtaatg ccgcgcggat ggcggcactg atgaaagcac
     4981 ggcagattat ggtttatacc cacgattcta ttggcttagg ggaagatggt ccgacgcacc
     5041 aggccgttga gcaactggcc agtctgcgct taacgccgaa tttcagcacc tggcgaccgt
     5101 gcgatcaggt ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacgggccga
     5161 cagcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta
     5221 aagagattgc tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc
     5281 tgattgccac cggttcagag atggaaatca ccctgcaagc ggcagagaaa ttagcgggag
     5341 aaggtcgcaa tgtacgcgta gtttccctgc cctcgaccga tattttcgac gcccaggatg
     5401 aggaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag
     5461 gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg
     5521 gttacgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga
     5581 atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatgg tgatcgccgg
     5641 atgctgattg ccggatgcga cgctgacgcg tcttatccgg cctacagcgt cttatccggc
     5701 ctacatgtcc ccgcaatttt gtttaacggg tgatccacaa cgtgggccag gcgtctggcc
     5761 catgccagtt atcgcaggtg ggttctgcgg cataacgcac caggcgaaaa cgctgaccgt
     5821 caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca
     5881 ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct
     5941 gaccgttgtt gaatggcaaa cgcaaccgaa ccgggcgcga ggctagtggc tttttacgcg
     6001 acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagcta atcatcatca
     6061 gcgctttatc atcagtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc
     6121 agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttgcgt tcttcgagtg
     6181 agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta
     6241 cgctgagcgg cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt
     6301 gctgagcatc aataaacaac aacgccgctt ttaagccact cagagaaatg gtctgattgc
     6361 cgtcgcgtaa ggtgatcgcc ttcccttcct gaatcatctg caaaaacgcg gtgatggttg
     6421 ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaacgcca
     6481 acggctcacc atctaacagc agccgtggcg ctatctctcc ttcagacgca tccggcgact
     6541 tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg cgccccggcg ctgcggctca
     6601 gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacgcagaag ttttggttat
     6661 tacaggtgac ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac accaaagacg
     6721 tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc
     6781 cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc
     6841 agatttgtcg gattgagcca ataataaaca acgaaatatt attgttattt ttcagtccat
     6901 taaatgtgac gtttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc
     6961 ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc
     7021 ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg
     7081 gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc
     7141 cacttcatag cccgtctctt caatcgcttc tttacgaatg catacttccg gttcgtcgtt
     7201 atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt
     7261 agcgacacgg aactgacgaa tcagaaccac gctctttttc ttcgcgttgt acaggaggat
     7321 cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt cgccatcttt
     7381 gcgcgtgaga tcgtaggtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc
     7441 tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatct
     7501 tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc
     7561 ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca
     7621 gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac
     7681 ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg
     7741 actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg
     7801 gcatgacaaa ttcactgccc tcaatcggta caggtcgccg acgcccctgg gcatccggct
     7861 cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgttca ttcagctcaa
     7921 gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct
     7981 ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg
     8041 cgccgtggcg cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca
     8101 cgacgttaag tccggcggta ttgataaacg gctcttccgg tagctcttcg agccccatca
     8161 cctgtttagt gttggcaatg aggaacggca gcgcgtcata aacgcccggc gcatcttcat
     8221 tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt
     8281 gttccaaaag cgaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa
     8341 tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag
     8401 aaggaatgcc gaaagtgagc aagccaccaa tttctggatg gcgatcgtac accgtcaccg
     8461 ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccgggcct gccccgataa
     8521 tcgccacccg cttgtccact ttggtgacat gacttaagtc aggacgccaa cctttcgcca
     8581 acgcctgatc tgaaatatag cgttcaatgt tgccgatagt tacagcaccg tgctcatcgc
     8641 gaatagtaca ggcaccttca cacaaacggt cttgcggaca aacgcgtccg gtaatttccg
     8701 gcagggtgtt ggtctggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca
     8761 gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct
     8821 cgccgcactt aaggcagcgc gaggcttccc gttgtgcctg gtcggcgcga aatggcagat
     8881 aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg
     8941 cgggcgttgc ctgcatttgt tcgactttac tcattaccgg catttcttgc gccgcggtac
     9001 tggcatgcca cggttgatgt tcctgacgcg cggtgcgcaa gcggcgggat ttcgccatgc
     9061 cggagagtgc gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcaacacagg
     9121 caggaccgtt ttcgcgcccc gcacaaaggt cgcatttatg cgccgtggct tttacttttc
     9181 ctgccgcgac gggtgtcagg acgatttgca tcgtaccaaa aggacaggcc accacgcagg
     9241 atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg
     9301 cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac
     9361 tacgttgctg ttgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga
     9421 catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac
     9481 attgctgact gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa
     9541 accttattct ttatatgagt gttgttatta cccgacttac aggggaatcg gcaatgttca
     9601 tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac
     9661 tgactaaatt gtgtttcagc taactggaac attaatgatt ttttgtggag aagacgcgtg
     9721 tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc
     9781 tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg
     9841 aggctatcaa tattgccgga tcgctacgta tgcagagtta ccgcctgggc tacgacttgc
     9901 aaagtggcag tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcac
     9961 cggtattaac caatctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc
    10021 atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt
    10081 atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc
    10141 actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta
    10201 ttttcacgct ggtctttttt actttgcgcc gtatacgcca tcaggtggtt gccccgctga
    10261 atcagctggt taccgccagt caacgtattg aacacgggca gttcgactcg ccgccgctgg
    10321 ataccagtct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccag atgtcgagcg
    10381 agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc
    10441 acgaggccaa gcgtcgtctg gaggtgttgt atcaatgttc ccaggcgctg aacaccagcc
    10501 agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgacaat gaagcggctg
    10561 aatatctgga gttaaatgtc ggtgaaaact ggcggattag cgaagggcaa ccaaacccgg
    10621 aattgccgat gcagatttta ccggtgacaa tgcaagagac ggtttacggc gaactgcact
    10681 ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagcgtttcg tcgatgctgg
    10741 gacgcggttt gtactttaat caggcgcaga agcattttca gcagttattg ttgatggaag
    10801 aacgtgcaac catcgcccgc gaattgcacg actcgctggc tcaggtactt tcttacttac
    10861 gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc gcacaaagta
    10921 tcatggccga tttttcccag gcgttgaatg atgcttatcg gcagttacgc gagctgttga
    10981 ctactttccg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg gaaatgctgg
    11041 atacgttaca aaatcaaacc agcgccaaac tgaccctcga ctgccgtctg ccaaccctgg
    11101 cgctggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg gtgctgaatg
    11161 cgatgaagca cgccaacgcc agcgaaatcg ccgtcagttg cgtcaccgcg ccggacggca
    11221 atcacacggt ttatatccgt gataacggga ttggcatcgg tgaaccgaaa gagcccgaag
    11281 gtcattatgg tctgaatatc atgcgcgaac gcgcggaacg gctgggtggg acgctgactt
    11341 tttcacaacc ttccggcggc ggcacgttag tgagtattag ctttcgctcc gcggagggtg
    11401 aggaaagtca gctgatgtaa tgcctcctac tgaccaaaga atacttgcac ttaaggttca
    11461 gtataaaagg gcatgataat ttacattaac tccttttttt ctccacgatt ggctcgtacc
    11521 ttgccgctac agtgaagcaa gtcaagccta caacgatacg cagaaacacg aggtcctctt
    11581 ttaatggcga atttctttat tgatcgcccc atttttgcct gggtgctggc aatcctgttg
    11641 tgtctgacag gtaccctggc gattttctca ttgcccgttg aacaataccc cgatctcgcg
    11701 ccaccgaatg tgcgagtgac cgctaactat cccggcgcat cggcccagac gctggaaaac
    11761 accgtgaccc aggttatcga gcaaaatatg accggcctcg ataatctcat gtatatgtca
    11821 tctcagagca gtggcaccgg tcaggcatct gtcactttaa gttttaaagc aggcaccgat
    11881 ccggacgaag ccgtgcagca agtacaaaac cagctgcaat cagccatgcg aaagttaccg
    11941 caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa tattctgacc
    12001 attgctttcg tctctaccga tggttcgatg gataaacagg atattgccga ttatgttgcc
    12061 agtaatattc aggacccgtt aagccgcgtg aatggcgtcg gggatatcga tgcctatggt
    12121 tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac tcaacagttt ccagatgacg
    12181 gctaaagatg tcactgatgc cattgagtca cagaacgcgc agattgcggt tgggcaactt
    12241 ggtggtacac cttccgtcga taagcaggcg ctcaacgcca ccattaacgc ccagtcactg
    12301 ctgcaaacac cagaacagtt ccgcgatatc accttgaggg tgaatcaaga cggctcagag
    12361 gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg agaaatacga ttatcttagc
    12421 cgcttcaatg gtaagccagc ctccgggctg ggggtaaaac tggcctccgg cgcaaacgaa
    12481 atggcgacag cggagctggt gctcaatcgt ctcgacgagc tggcgcagta tttcccgcac
    12541 ggactggaat acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc cattgaagac
    12601 gtggtgaaaa cgctgctgga agctatcgct ctggttttcc tcgttatgta tctgttcctg
    12661 caaaacttcc gcgccacgct gatacccact atcgccgtgc cggtggtgtt gatgggaacc
    12721 ttctccgtac tttacgcctt cggttacagc gtcaacacct taaccatgtt cgcgatggtg
    12781 ctggcgatcg gtctgctggt ggatgacgca atcgtggtgg tggaaaacgt cgaacgtatt
    12841 atgagtgagg aaggactcac tcctcgcgaa gccacacgta aatcgatggg gcagatccag
    12901 ggggcactgg tcgggattgc gatggttctt tcggcggtat ttgtaccaat ggccttcttc
    12961 ggcggcacca ccggtgccat ctatcgccag ttctctatta ccattgttgc ggcgatggtg
    13021 ctgtcagtac tggtagcgat gatcctcact ccggctctgt gtgccacact acttaagcca
    13081 ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg cctggtttaa ccagatgttt
    13141 aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg tagcctgcgc
    13201 tggattgtga tttatgtcct gctgcttggc ggcatggtgt tcctgttcct gcgtttgccg
    13261 acgtcgttct taccgctgga agaccgtggc atgtttacta cctcggtaca attgcccagc
    13321 ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa tcgagaaata ttacttcacc
    13381 catgaaaaag acaacatcat gtcggtgttt gccactgttg gttctggccc cgggggtaac
    13441 gggcaaaacg tggcgcgaat gtttatccgc ctgaaagact ggagcgaacg cgacagtaag
    13501 accggcacct catttgccat tatcgagcgt gcaacgaagg cgtttaacaa gattaaagaa
    13561 gctcgcgtta tcgccagcag cccgccagca attagcggtc ttggtagttc tgcaggtttt
    13621 gatatggagt tgcaggacca cgctggagca ggtcacgatg cgctgatggc agcacgtaac
    13681 cagttgctgg cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca taacggcctc
    13741 gatgacagtc cgcagttgca gattgatatc gaccagcgta aagctcaggc gctgggcgtt
    13801 gctatcgacg atattaacga cacactgcaa accgcctggg gttcgagcta tgtgaatgac
    13861 tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc gtatcgcatg
    13921 ctgccagatg acattaatct ctggtatgtc cgaaataaag atggcggcat ggtgcccttc
    13981 tctgctttcg cgacctcacg ctgggagaca ggctcgccgc gtctggaacg ctataacggt
    14041 tattctgcgg ttgagattgt tggggaagcc gcaccggggg tcagtaccgg tacggcgatg
    14101 gatattatgg aatcgttagt gaagcagttg ccaaacggct ttggtctgga gtggacggcg
    14161 atgtcgtatc aggagcggct ttccggcgcg caggctccgg cgctgtacgc catttccttg
    14221 ctggtagtat tcctgtgtct ggcggcattg tatgaaagct ggtcggtacc gttctcggta
    14281 atgctggtcg tgccgctggg ggttatcggc gcgctgctgg caacctggat gcgcgggctg
    14341 gaaaacgacg tttacttcca ggtgggcctg ttaacggtca ttggtttatc ggcgaaaaac
    14401 gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga cctgtttgaa
    14461 gcgacgctcc acgcctgccg tcagcgttta cgcccgattc tgatgacctc gctggcattt
    14521 atcttcggcg tattgccaat ggcaaccagc acgggtgccg gttccggtgg tcagcatgcg
    14581 gtgggtactg gcgtaatggg cgggatgatt tcagctacta ttctggctat ttacttcgtg
    14641 ccgctgttct ttgtgctggt gcgccgccgc ttcccgctga agccgcgccc ggaataagca
    14701 ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact ttccgattaa gaacctgctc
    14761 agcgggttct tgctgtttat actttgtctc gggaattact tacgaagcat aacttcgata
    14821 aagtctttcc agttccccag ttcacgttca atcataacaa cctctcttat aattatgggt
    14881 attctacgga aacaatatac cgtggtgaag ctaatttact cgattgctgc gatgactacc
    14941 tccgggggac aaaccttatg taaatactat ggtcctgcgg tgataatttg tatgtgatac
    15001 acagcaacat ttcgagatat tcatacggca tctaatactg atttaattct gg
//
LOCUS       DAZFWP020000119        14986 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFWP020000119 DAZFWP020000000
VERSION     DAZFWP020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.119"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            122..364
                     /gene="yjbE"
                     /locus_tag="ACHK0Z_RS17820"
                     /old_locus_tag="pgaptmp_003564"
     CDS             122..364
                     /gene="yjbE"
                     /locus_tag="ACHK0Z_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU4503523.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            478..1116
                     /locus_tag="ACHK0Z_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             478..1116
                     /locus_tag="ACHK0Z_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU4503524.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1113..1850
                     /locus_tag="ACHK0Z_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             1113..1850
                     /locus_tag="ACHK0Z_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4503525.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAASRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1850..3946
                     /locus_tag="ACHK0Z_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             1850..3946
                     /locus_tag="ACHK0Z_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4503526.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3993..4271)
                     /locus_tag="ACHK0Z_RS17840"
                     /old_locus_tag="pgaptmp_003568"
     CDS             complement(3993..4271)
                     /locus_tag="ACHK0Z_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503527.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4287..4415
                     /locus_tag="ACHK0Z_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             4287..4415
                     /locus_tag="ACHK0Z_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503528.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            4541..4951
                     /gene="psiE"
                     /locus_tag="ACHK0Z_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             4541..4951
                     /gene="psiE"
                     /locus_tag="ACHK0Z_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU4503529.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="ACHK0Z_RS17855"
                     /old_locus_tag="pgaptmp_003571"
     CDS             complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="ACHK0Z_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU4503530.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLNESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6842..7732)
                     /gene="malG"
                     /locus_tag="ACHK0Z_RS17860"
                     /old_locus_tag="pgaptmp_003572"
     CDS             complement(6842..7732)
                     /gene="malG"
                     /locus_tag="ACHK0Z_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU4503531.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7747..9291)
                     /gene="malF"
                     /locus_tag="ACHK0Z_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             complement(7747..9291)
                     /gene="malF"
                     /locus_tag="ACHK0Z_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU4503532.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9445..10635)
                     /gene="malE"
                     /locus_tag="ACHK0Z_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             complement(9445..10635)
                     /gene="malE"
                     /locus_tag="ACHK0Z_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU4503533.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11000..12115
                     /gene="malK"
                     /locus_tag="ACHK0Z_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             11000..12115
                     /gene="malK"
                     /locus_tag="ACHK0Z_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU4503534.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12187..13527
                     /gene="lamB"
                     /locus_tag="ACHK0Z_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             12187..13527
                     /gene="lamB"
                     /locus_tag="ACHK0Z_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU4503535.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAVTRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAYVDEKFSYDINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTLMEIGYDNVKSQRTGDTNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTDKAKGTPNYGKAVPANFEGGSFGRGDSDEWTFGAQMEIWW"
     gene            13770..14690
                     /gene="malM"
                     /locus_tag="ACHK0Z_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             13770..14690
                     /gene="malM"
                     /locus_tag="ACHK0Z_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU4503536.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAASAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3705 a         3857 c         3941 g         3483 t
ORIGIN      
        1 tatcctaaaa ccccatctct aattccccgc gccgtaattc gcatgcttta gttgtgtata
       61 ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa gggaaattgt
      121 tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg
      181 ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg
      241 gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc
      301 aaccggtggc ggtgatggtt ctaataccgg gaccaccaca accacgacca ccagtaccca
      361 gtaataaagt atgtatcccc aaaataattt gagtcattgc atctgtggct tgaagtatga
      421 agggatttac cataaccaca ctccggtgtg gttattctgc ccctctggag aagagtcgtg
      481 aagcgacctg cactcattct tatctgcctg ctattacagg cctgttcagc cacgactaaa
      541 gagctgggca attcactgtg ggacagtctg ttcggcacgc caggcgtaca gctgacggac
      601 gatgatattc aaaatatgcc ctacgccagc cagtacatgc agcttaatgg cgggccgcaa
      661 ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa aatgggtcac tcaggatcaa
      721 gccactcttg tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg
      781 attgaagtga ataaccttgc cgccgacccg ctgattaaac ccgcacaaat tgttgatggt
      841 gcaacatgga cgcgcacgat gggctggacc gagtaccagc aggtacgcta cgccaccgca
      901 cgctcagtct tcaaatggga tggcaccgat accgtcaagg tcggcagcga tgaaaccccg
      961 gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc gctggcataa ccgctactgg
     1021 atcgacagcg aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg
     1081 gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa acaaactatt gtcgcattga
     1141 ttttgagtgt gggggcgtca tcggtcttcg cggcgggaac cgtcaaggta ttcagcaatg
     1201 gcagcagtga ggccaaaacg ctgacgggcg cagagtattt aatcgatctg gtaggccagc
     1261 cgcggctggc aaacagttgg tggcctggtg cggtgattag cgaagagctg gcaacggcgg
     1321 cagcatcgcg gcagcagcag gcgttgctga cacggctggc agaactggct gcagattcca
     1381 gcgccgacga tgccgccgcg attaacgcct tacgccagca aattcaggcg ttgaaagtga
     1441 cgggcaggca aaaaatcaat cttgatcctg atatcgtccg cgttgccgaa cgcggtaacc
     1501 caccgttgca gggcaactac acgctgtggg tcgggccacc accgtccacg gtcacgctgt
     1561 tcgggcttat cagccgtcct ggcaagcagc cattcactcc aggtcgcgac gtggcgagct
     1621 atctctccgg gcagaacctg cttagcggtg cggatcgcag ctacgcgtgg gtggtttacc
     1681 cggacggacg cacgcaaaaa gcgccggtgg cttactggaa caagcgtcac gtagagccga
     1741 tgcccggcag cattatttat gttggcctcg cggactccgt ctggagtgag acgcctgatg
     1801 cccttaacgc cgacattctt cagactctga cgcagcggat accccaataa tgaaaaaaag
     1861 acatctgctt agcttactgg cgctgggcat tagcacagct tgctacggcg aaacatatcc
     1921 tgcgcccatt ggtccgtcgc agtcggattt cggtggcgta ggattattac aaacgcccac
     1981 cgcgcgcatg gcgcgggaag gggagttgag cctgaactat cgagataacg atcagtaccg
     2041 ttattactca gcttcagtgc aactcttccc gtggctggaa acaacgctgc gctacaccga
     2101 cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga
     2161 taaagccttc gatctcaaac tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc
     2221 ggttggtgcg cgggatatcg gcggtacggg gctgtttgat gcggaatatc ttgttgccag
     2281 caaagcctgg gggccgttcg attttacgct cggcctgggc tggggatatc tgggcaccag
     2341 cggtaatgtg aaaaatccgc tctgttcagc cagtgataaa tattgctatc gcgataacag
     2401 ctacaaacag gcgggatcta tcgacggtag ccagatgttc cacggtcctg cctcactgtt
     2461 tggcggcgtg gaataccaga cgccctggca accgctgcgc ctgaaactgg agtatgaagg
     2521 caataattat cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg
     2581 tgcgatttat cgcgttaccg attgggccga cgttaacctt agctatgaac gtggcaacac
     2641 ctttatgttt ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt cctacatcga
     2701 taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc attcggtggt
     2761 ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt gccgatccgc agatccaggc
     2821 gaaaggcgat acgctgtatg ttaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg
     2881 gatcatccgt gctaatcgga tcgtgatgaa tgatctgccg gatgggatca aaacgatccg
     2941 cgttacggaa aatcgcctta acatgccgca ggtgacgacg gaaaccgatg tcgccagcct
     3001 gaaaaatcat ctcgccggag agccgttggg ccacgaaacg acgctggcgc aaaaacgcgt
     3061 cgagccagtg gttccgcagt ccaccgagca gggctggtat atcgacaaat cacgctttga
     3121 tttccatatc gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact tttacatgta
     3181 tcagctgggc gtgatgggaa cggcagattt gtggctgacg gaccatctac tgaccaccgg
     3241 cagcctgttt gcaaatcttg ccaacaacta cgacaagttt aactacacca atccaccgca
     3301 ggactcgcac ttaccgcgcg ttcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta
     3361 tgtgaataac ctgcaagcca actacttcca gcatctgggc aacggcttct acggtcaggt
     3421 ctacggtggt tatctcgaaa ccatgtttgg cggtgcgggg gcagaagtgt tgtatcgccc
     3481 gctggacagc aactgggcgt ttggtctgga tgccaactac gttaaacagc gcgactggcg
     3541 tagtgcaaaa gatatgatga aattcaccga ctacagcgtg aaaaccggac atctgaccgc
     3601 ctactggacg ccatctttcg ctcaggatgt gttagttaaa gccagcgtcg ggcagtatct
     3661 ggcaggggat aaaggcggca cgctggagat cgccaaacgc tttgatagcg gcgtggtggt
     3721 gggtggctat gccacgatca ctaatgtttc aaaagaggag tacggcgaag gggacttcac
     3781 caaaggcgtg tatgtctcgg taccgttgga tctcttctcg tctggcccga cacgcagccg
     3841 tgcggcgatt ggctggacgc cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt
     3901 ccagttgtat gacatgacca gcgaccgtag cgtcaatttc cgctaagtca tgggaaaggt
     3961 gccagttttc gcaatcactg gcaccctcat tcttaatggc ctgttgagcc atcaggaacg
     4021 cagttcaccc ccggtttgcg atctggtgtg taaaggcgaa aacattctgt cttcctgtcc
     4081 gtcgatatcg gttgcagatc tggttgcctg agcgactggg ccatcattgg gctggagaca
     4141 ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg gaccagcata agcaggaaga
     4201 acagagactg ataacatcaa agcagcaaaa taaggcttca tttttaccac ctttatcagg
     4261 ttacgtttca tttgttccag aggaacatgg tcgatttttc gcgcattgct ggtggctggg
     4321 aatcacctga atgggtgatt tttgaattac cggctttggt gcggtttgtc ttgccggatg
     4381 cgccgccagg cgcggcttat ccggcctacg ggtaggtata tccggctttg gtggaggcgc
     4441 gctccaaatc caggttgaac aaaacataca caaaaaatat agatctccgt cacatttttg
     4501 cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac tctctcgtcc
     4561 gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc tgttgtgcct
     4621 cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggcag atgtgctgtt
     4681 cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt actttctcta
     4741 tttcgaattt atcgcgctga ttgtgaaata ctttcagtcc ggttttcact tcccgctacg
     4801 ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc
     4861 gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca ctctgtggtt
     4921 gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt
     4981 gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag
     5041 agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt ccacataaat
     5101 agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt gtggaaatgg
     5161 gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc
     5221 agccactggg ccgccaccgc gattgccagc gctttaccac gaatggcatt cgggaagatt
     5281 tccgacagca gaacccagca taccggaccc caggacatgg caaaggcggc aacatagaac
     5341 agcatcgaca gtagcgccac aatacccggt gcctgagtgt aaaacgcggt accgaggcta
     5401 aacataccga ttgccattcc gagcgcgccg ataatttgca gtggcttacg accaaattta
     5461 tccaccgtca taattgccag aacggtgaag gtgaggttga taactccgac aataatggtc
     5521 tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac
     5581 aacaccacat tgatgccgac aaattgctgg aagatggaga gcataacgcc gattacaatc
     5641 acgcccacgc caaacatcag cagacgacca ccggttttgc ggccatgatc cagggagtgt
     5701 ttaatttcct gtactgcctg agttgcaagc gtgttgccca taattttgcg caggatactt
     5761 tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg cacggtatac
     5821 agcagcatta agaacagcag tgcagggata cattccgagg caaacatata acgccagccg
     5881 tcagtattca gccagctggc atcaccggaa cgggcaataa aatagtttac gcagtaaact
     5941 aaaagttgcc cgaaaataat cgcaaactgg ttaaatgaaa ccagtttccc gcgaatatga
     6001 gctggagcca gttccgcaat atacattggt gaaagcattg aggctaaacc aacgccaata
     6061 ccgccaataa tgcgataaat aacaaattcc gggacataac ctgccagata aacaggcact
     6121 gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa
     6181 ataaaaaaca ggacagcagc aatcttaagt gaatcacgac gaccgaagcg gttactgcaa
     6241 taaccaccga gggcaccgcc gatgatgcaa ccaatcagag cgctggctac gcaaaaccct
     6301 aacagggagt tggcagcgga ttcatttaag ttttgtggag caacaaagac ggtattgagt
     6361 gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta
     6421 gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc
     6481 cttagatcat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc
     6541 aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta attgtgataa
     6601 ttatccaaaa ttgataaaaa caatactatt gccgtgattc agagcacgaa agagaattat
     6661 cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatgggaa tattaaccgg
     6721 cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc cgaataatag
     6781 tcacaagttg ggataacgta agttgagggt gcagcggcat aacattggca gaacaacatc
     6841 tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga gccagcaaga
     6901 agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca ccccacaggt
     6961 agttttgcgg gttgaggtat tgctgcatcc ccacggccag ggtgtagcta tttacgtcac
     7021 gcagtaacag cgacgcgacc ggaacttcag taatggcagc gataaacgac aggatgaaca
     7081 ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc tgccacggtg
     7141 tcgcaccatc cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg aaatagcctt
     7201 tgatggtcca gacatgcagc gcaatcccac ccagatacgc gaaaattacg ccgccatgag
     7261 tattcagacc aatgaatgga atgtactcac ccagacgatc aaacaacgca tacaacgcga
     7321 ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc agcgtcgctt
     7381 tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc
     7441 caatcgcgga aatcccggcg acctttaccg agttccacag ccacagcagt accgggaatg
     7501 gcggtggcgt aatgcgacca tcagcctgtt caacgctaaa acctaacgcc agtttccagt
     7561 gatcccagga gatttgctcc gggatcaggc taccggtcgc gaagtttccc tgacgcagcg
     7621 agatagcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt agcagtagca
     7681 ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc atttttgtta
     7741 tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca gcgcacccac
     7801 cagcaggaag atcagcgtgg caattgctgc cgccagaccg aagtcctgac ccccgccgcc
     7861 ttcaaaagcg atacggtagg tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt
     7921 cgtgccaaga cgatccgggc cgccgttggt taacagctga atcagcacga agttgttaaa
     7981 gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt
     8041 aatcttaaag aagttctgga acggacctgc gccatccatt gctgaggctt catacaaatc
     8101 gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat aacccagcca
     8161 ggtattgacg ataatcagca tcgtgcgggc ggtggtcgga tcgctgaacc aggcgggctt
     8221 cacgccaaac agcgcgctca acatcatgtt gatttcaccg aagctctggt taaacaatcc
     8281 tttgaaaatc aagattgaaa tgaacgatgg caccgcgtag ggcagaatca gcaggacgcg
     8341 atagaccgct ttgccgcgca acgcttccca ctgcaccaga catgccagaa ccatgccgac
     8401 cgccaccgtt aaaaagacag tgatcagcga gaacaccacg gtccagacga aaatggcgag
     8461 gaacggtttc tgaatgcctt cgtcggtaaa gacgcgggta aagtttttcc agccggtggt
     8521 cacggtgtaa ccggggctta gcttttcatc accccagttg ccgtcggcgg taatggactg
     8581 gtaaaagcca atttggttat tcggacgata tttcacgccg ctctgattat tcgtcaacgt
     8641 gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca gggagctcat
     8701 catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct gacgattctg
     8761 ggtaatcacg cgcagattcg cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg
     8821 cagtttttgc tcgccgccaa atttaaacgc gtcggagagg taatttttgc cggtttcgcc
     8881 gtcgctgagc gccagttgcc actcatcgcc cgccgggtaa agaccaaagt tataggtttt
     8941 gcctgcttgc caggagcgat ctaacaacac ttcctgcgca cgttcaaaag tcagctggtt
     9001 agtgctgctg tagttggtga aggcaatggc gatggtgcag accagaggga agaggacgaa
     9061 taatcccatt ccagccattc ccgggtaaac atagcgccag gcgtaggctt tacgattggc
     9121 gaaaatatac agccccgctg aactcaatat cagcgtggta atggcgaaca ggtattcccc
     9181 ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac ctagcactga
     9241 ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca tggggttctt
     9301 cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc ctgatgcgac
     9361 actgatgcgt cttatcaggc atacatgcgt ttcagttttg taggccggac aaggcgttca
     9421 cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt ctttcagggc
     9481 ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca
     9541 gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatggt
     9601 ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ccactgcacc
     9661 cagcggtttg tctttattga ccgcttccag accttcgtca gtcagcagat agttttcgag
     9721 gaactctttc gccagctctt tgttcggact ggcggcgtta ataccggcgc tcagcacgcc
     9781 aacgaacggt ttagatggtt gacccttgaa ggtcggcagt accgttacac cataattcac
     9841 tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc
     9901 tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt
     9961 aataaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc
    10021 tttaatatcg tacttgccgt tttcatactt gaacgcataa cccccgtcag cagcaatcag
    10081 cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt
    10141 cagttcttta tccagcgccg ggatctcttc ccaggttttt ggtgggttcg gcagcagatc
    10201 tttgttataa atcagcgata acgcttcaac agcgatcggg taagcaatca gcttgccgtt
    10261 gtaacgtacg gcatcccagg taaacggata cagcttgtcc tggaacgctt tgtccggggt
    10321 gatttcagcc aacaggccag attgagcgta gccaccaaag cggtcgtgtg cccagaagat
    10381 aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca gtttatccgg
    10441 atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga cttcagcgag
    10501 accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt cgattttggc
    10561 gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc
    10621 tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa acacctaaac
    10681 ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta cgccccaccg
    10741 ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggagatgcgc acataaaatc
    10801 gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt tagttcacag
    10861 aagccgtgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg tggaggattt
    10921 aagccatctc ctgatgacgc atagtcagcc catcatgaat gttgctgtcg atgacaggtt
    10981 gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga aagcctgggg
    11041 cgaggtcgtg gtatcgaaag atatcaatct cgatatccat gaaggtgaat tcgtggtgtt
    11101 tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg ggcttgagac
    11161 gatcaccagc ggcgacctgt tcatcggtga gaaacggatg aatgacacac cgccagcaga
    11221 acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt cagtagcaga
    11281 aaacatgtca tttggcctga aactggctgg cgcaaaaaaa gaggtgatta accaacgcgt
    11341 taaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct
    11401 ctctggcggt cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg agccaagcgt
    11461 atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat
    11521 cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca cccacgatca
    11581 ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc gcgtggcgca
    11641 ggttgggaaa ccgctggagc tgtaccacta tccggcagac cgttttgtcg ccggatttat
    11701 cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa tcgatcaagt
    11761 gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctgccagttg aaagccgtga
    11821 tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccgagtga
    11881 tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac
    11941 ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt
    12001 ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct
    12061 gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc
    12121 ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga
    12181 gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta
    12241 atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg
    12301 acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt
    12361 cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag
    12421 ggcgataaga gcttctattt cgacactaat gtggcctatt ccgtcgcaca acaaaatgac
    12481 tgggaagcta ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa tctgatcgaa
    12541 tggctgccag gctccaccat ctgggctggt aagcgcttct accaacgtca tgacgttcat
    12601 atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga aaacatcgat
    12661 gttggcttcg gtaaactctc tctggcagta acccgctcct ctgaagcagg tggttcttcc
    12721 tctttcgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga cgttttcgat
    12781 gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg tgtcgactac
    12841 ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa agacggctgg
    12901 ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag
    12961 tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc tggtgttgca
    13021 tatgttgacg aaaaattttc ctatgacatt aacaacaacg gtcacatgct gcgtatcctc
    13081 gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg tatgtaccag
    13141 gatatcaact gggataacga caacggcacc aagtggtgga ccgtcggtat tcgcccgatg
    13201 tataagtgga cgccgatcat gagtaccctg atggaaatcg gctacgacaa cgttaaatct
    13261 caacgtactg gtgataccaa caaccagtac aaaatcacac ttgctcaaca atggcaggct
    13321 ggcgacagca tctggtcgcg tccggctatt cgtgtttttg ccacctacgc caagtgggat
    13381 gagaaatggg gttacgatta caccgacaaa gctaaaggta ctccgaacta cggcaaagcc
    13441 gttccagcta acttcgaagg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc
    13501 ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag gtgcttgcgc
    13561 cgcatccggc atccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc tacaacggct
    13621 gtcaaatgta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca ggttgtcatt
    13681 atctgaaagg ggcgcaagcc cctctgatta tcgggtttag cgcgctattg cctggctacc
    13741 gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc atcgccctct
    13801 gtttatcagc agggttactg gcaagcgcgc ctggaatcag ccttgccgat gttaactacg
    13861 taccgcaaaa caccagcgac gcgccagcca ttccatctgc cgcgctgcaa caactcacct
    13921 ggacaccagt cgatcaatct aaaacccaga ccacccaact ggcgaccggc ggccaacaac
    13981 tgaacgttcc cggcatcagt ggtccggttg ctgcgtacag cgtcccggca aacattggcg
    14041 aactgaccct gacgctgacc agcgaagtga acaaacaaac cagcgtattt gcgccgaacg
    14101 tgctgattct tgatcagaac atgaccccat cagccttctt ccccagcagt tatttcacct
    14161 accaggaacc aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc ctgacaccgg
    14221 cgttggggca gcaaaaactt tatgttctgg tctttaccac ggaaaaagat ctccagcaga
    14281 cgacccaact gctcgacccg gctaaagcct acgccaaagg cgtgggtaac tcgatcccgg
    14341 atattcccga tccggttgct cgtcatacca gcgatggctt actgaaactg aaagtcaaaa
    14401 cgaactccag ctccagcgtg ttggtaggac cactatttgg ttcttccgct ccagctccgg
    14461 ttacggtagg caacacggcg gcaccagctg tggctgcatc cgctccggca ccggtgaaga
    14521 aaagcgagcc gatgctcaac gacacggaaa gttattttaa taccgcgatc aaaaacgctg
    14581 tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga tgaagctgaa cgcctgggat
    14641 cgacatctgc ccgttccacc tttatcagca gtgtaaaagg caaggggtaa ttacgcccca
    14701 cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta tgcttccttc
    14761 ttgggatatg agcgattttt tatagtaact cacttcttct tcactaagaa tgtccattat
    14821 ctcaatgcct tatcagagat tcttttcctt tcgccggtag tgtctggaca ttcaggctac
    14881 ttttccaggt tattttattt ctgttatgca gaggttttat gataagtcat atcccaaatt
    14941 ctggcggcaa taactctttg atgaaacatg atgtggtgca aggaaa
//
LOCUS       DAZFWP020000120        14909 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFWP020000120 DAZFWP020000000
VERSION     DAZFWP020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.120"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..759
                     /locus_tag="ACHK0Z_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             <1..759
                     /locus_tag="ACHK0Z_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4503537.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACHK0Z_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACHK0Z_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU4503538.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACHK0Z_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             2581..3900
                     /locus_tag="ACHK0Z_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU4503539.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISTPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4289..5671
                     /gene="dosC"
                     /locus_tag="ACHK0Z_RS17905"
                     /old_locus_tag="pgaptmp_003581"
     CDS             4289..5671
                     /gene="dosC"
                     /locus_tag="ACHK0Z_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU4503540.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5696..8095
                     /gene="dosP"
                     /locus_tag="ACHK0Z_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /gene_synonym="pdeO"
     CDS             5696..8095
                     /gene="dosP"
                     /locus_tag="ACHK0Z_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HGU4503541.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8353..8934
                     /gene="ddpX"
                     /locus_tag="ACHK0Z_RS17915"
                     /old_locus_tag="pgaptmp_003583"
     CDS             8353..8934
                     /gene="ddpX"
                     /locus_tag="ACHK0Z_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU4503542.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8948..10498
                     /locus_tag="ACHK0Z_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             8948..10498
                     /locus_tag="ACHK0Z_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4503543.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVESDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10500..11522
                     /locus_tag="ACHK0Z_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             10500..11522
                     /locus_tag="ACHK0Z_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4503544.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGYLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11519..12415
                     /gene="ddpC"
                     /locus_tag="ACHK0Z_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             11519..12415
                     /gene="ddpC"
                     /locus_tag="ACHK0Z_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU4503545.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12412..13398
                     /locus_tag="ACHK0Z_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             12412..13398
                     /locus_tag="ACHK0Z_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4503546.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQLLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13391..14317
                     /locus_tag="ACHK0Z_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             13391..14317
                     /locus_tag="ACHK0Z_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU4503547.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14373..14804)
                     /gene="osmC"
                     /locus_tag="ACHK0Z_RS17945"
                     /old_locus_tag="pgaptmp_003589"
     CDS             complement(14373..14804)
                     /gene="osmC"
                     /locus_tag="ACHK0Z_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU4503548.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3624 a         3737 c         3806 g         3742 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggttgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggtgtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta catagtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tttctcggct cgactggcca ccggtttcct cggttaacat tagtaccccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gccgaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta tttctatacg
     3301 gagtcaccgg gttcactgct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccatgc ttactttgta acaagccggt gctttaccca ccggcgtgtt acaccttgtg
     3961 aaatatccca ctagtaatca tgcttacata agtcaaatta accacgcaat taaccacgca
     4021 attaaccaca cttagttaaa cagctataaa tccctaataa aaatgtatga atatgaaaaa
     4081 ccatacgata ttcgctgtca ccgaaaaata attacgatgg gctatactct tctgaagaac
     4141 agtagcgtag attgaaactg tcctaagtcg cttatttctt ttcagtatat cttcatattt
     4201 caggagaata tggtgcagtt agtcgatctc gcacgttgcg tttcgttttg attccgtctg
     4261 atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa tgaaagatga
     4321 gtggaccgga cttgtcgaac aggcagatcc tctcattcgt gctaaagccg cagaaattgc
     4381 ccttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca tcgacccgca
     4441 tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg cgatggaacg
     4501 ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa tacaaatcca
     4561 gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg tcgagatggg
     4621 gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt attctgccgc
     4681 agaaaaactt caggtctacc atttctcgat taacagtatt gatatcgcta tggaagtgat
     4741 gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa actatcgtat
     4801 cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct caatactttc
     4861 atgggaaata gatattatct ataaaatcct gctggattct gatttaggca gtagtttgcc
     4921 tttaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt attttagcgg
     4981 cattgccgaa gtaggccata tctcccgtct gattcaggat ttcgacggta ttttcaatca
     5041 aaccatgcgt aacaccagga atttgaataa cagaagtttg cgggtgaagt ttctattaca
     5101 gataagaaat accgtatcgc aaattattac cttgctgcgt gaattgtttg aagaagtatc
     5161 gcgccacgaa gtcggtatgg atgtactgac gaaattactt aaccgccgtt tcctgccgac
     5221 tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt cagtgctgat
     5281 tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca ctggcgatga
     5341 aattctgcgt aaagtctctc aggcctttta tgacaacgtc cgcagtagtg attatgtttt
     5401 ccgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa acgaaacgtt
     5461 acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag ccgcaaacgg
     5521 cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc atcctgacta
     5581 tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac gaggtagaaa
     5641 ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg taatcatgaa
     5701 gctaaccgat gcggataatg ccgccgatgg catttttttc cccgcccttg agcaaaatat
     5761 gatgggtgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc ccgccgcaga
     5821 gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata tgctgattcc
     5881 gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag gcggtaaagc
     5941 gcgtgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca gtaaaatctg
     6001 gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc tggcgctggt
     6061 acgggatgcc agcgtagaaa tggcgcaaaa agaacagact cgacaattga ttattgccgt
     6121 tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg tgcagtgcaa
     6181 tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta tgcagcccga
     6241 tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac aacagttgct
     6301 atggaaaacc gcccgcgatc aggatgaatt tctgctgttg acgcgcaccg gtgaaaaaat
     6361 ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc agaacctggt
     6421 aatgaccttc tcggatatca ccgaagaacg gcaaatccgc cagcttgaag gcaatattct
     6481 cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt gtcgtaacat
     6541 cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg ggatgccgat
     6601 acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct ggtcagcgac
     6661 cattcgtcag cgtgatggcg cgcctgcggg gatcctgcaa attaaaacct cgtcaggagc
     6721 agaaaccagc gcctttatcg aacgcgtggc agatatcagt cagcatatgg ccgcgctggc
     6781 gctggaacag gaaaaaagcc gccagcatat tgaacaactc atccaatttg atccgatgac
     6841 cggtctgcca aatcgcaata acctgcacaa ttacctcgat gacctggtcg acaaagccgt
     6901 ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg atagccttgg
     6961 ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg aaaaactcaa
     7021 accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga gcctggaaaa
     7081 cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca gcaagccgat
     7141 aatgattgac gataaaccct tcccgcttac cttgagtatt ggcatcagct acgacgtggg
     7201 taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata ttcgcaagaa
     7261 tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa aagagcgttt
     7321 ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac ttgtttacca
     7381 gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg cgcgctggca
     7441 cgatccccag catggtcatg tgcccccttc acggtttatt cctctcgcag aagagattgg
     7501 tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt tagcagaatg
     7561 gcgtagccag aatattcata tcccggcgct ttccgttaac ttgtcggcac tgcactttcg
     7621 cagtaatcaa ctgcctaatc aggtgtctga tgcaatgcac gcctggggta ttgacggcca
     7681 ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg aaatctttaa
     7741 gcgcattcag atcctgcgtg atatgggcgt gggcttatcg gtagatgatt ttggtacggg
     7801 cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa ttgacaaaag
     7861 ttttgtcgat cgttgtctga ccgaaaaacg catccttgcc ttacttgaag ccattaccag
     7921 cattgggcaa agcctcaatt taaccgtcgt ggcggaaggg gtcgaaacca aagagcaatt
     7981 tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt cccgccccct
     8041 acccgccgaa gaaattccag gctggatgag cagcgtgtta ccgctgaaaa tctgacaaat
     8101 tcctctcgcc cgcactcgcg ggttttcatt taacgtgaca ctgtcactta aatactgtga
     8161 tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc aaaactgcac
     8221 tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag cattctggcg
     8281 cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt ttgtcttata
     8341 caggaaccgt ttatgtcgga taccaccgaa ctggttgatt tagccgtaat cttccctgat
     8401 ctggagatcg aattgaaata cgcctgcgct gataacatca caggtaaagc tatttatcag
     8461 caagcgcgtt gtctgctaca caaggatgcg attaccgcgc tggcgaaaag tatcagtatc
     8521 gcccagctgt caggcttaca actggtgatt tacgatgcgt atcgcccaca acaagcacag
     8581 gcgatgttgt ggcaagcctg cccagacccg caatatgttg ttgatgtgac ggtcggttct
     8641 aatcacagcc gtggcacggc gatcgacctg acgcttcgtg atgagcacgg gaacatcctc
     8701 gatatgggcg cagggttcga tgaaatgcac gagcgctccc atgcctatca cccttccgtc
     8761 ccgcccgccg ctcagcgcaa tcggctgttg ctgaatgcga taatgactgg tggcggtttt
     8821 gtcggtatct ccagcgaatg gtggcacttc gaattacccc aggcagcgag ttaccctctg
     8881 ctcgctgatc aattcacctg ttttatatcc cctggcacac aacacgtcag ttaatcagga
     8941 aactgtcatg aagagatcga taccgtttcg tcccacattg ctcgcgctcg tccttgccac
     9001 aaatttcccg gttgcgcacg ccgccgtacc gaaagatatg ctggtgattg gtaaggccgc
     9061 cgatccacaa accctcgacc cggcggtaac aatagataat aacgactgga cggtgaccta
     9121 cccgtcttat cagcggctgg ttcagtacaa aacggacggt gataaaggct caaccgacgt
     9181 tgaaagcgat ctggcaagta gctggaaagc gtctgacgat caaaaagagt ggacgttcac
     9241 cctgaaggac aatgctaaat ttgccgatgg cacaccggtc actgccgaag cagtaaaact
     9301 ctcttttgag cggctactaa aaatcggcca agggccagca gaagcatttc ccaaagattt
     9361 aaagattgat gctcccgacg aacatacggt gaagtttacc cttagccagc cattcgcacc
     9421 gttcctctac acgctggcga atgacggtgc atccattatc aatccggcgg tcttaaagga
     9481 acatgcagcg gatgatgctc gcggcttcct cgcgcaaaat accgccggtt ccggaccatt
     9541 tatgctgaaa agctggcaaa aaggtcagca attagttctg gtgccaaatc cgcattaccc
     9601 cggcaataaa ccgaacttta agcgagtatc ggtaaaaatt atcggtgaaa gtgcctcccg
     9661 tcgcctgcag ctctcccgtg gcgacattga cattgccgat gcgctgccgg tggatcaact
     9721 caacgccctg aagcaggaaa ataaagtcaa tgtggcagag tatccgtcac tgcgcgttac
     9781 ctatctgtat ctgaataaca gcaaagcgcc tcttaatcag gcggatctgc gtcgggccat
     9841 ttcctggtct accgattatc agggcatggt taacggcatt ctgagtggta acggaaaaca
     9901 gatgcgcggc ccgattccgg aaggcatgtg gggctacgat gcgacggcaa tgcaatacaa
     9961 ccatgacgaa acgaaagcca aagccgaatg ggataaagtg acgagcaaac ccaccagcct
    10021 gacgtttctc tactccgata acgatccgaa ctgggagcct attgctttgg cgacacaatc
    10081 cagtctcaac aagctgggca tcaatgtgaa gctggaaaag ctggcgaacg ccaccatgcg
    10141 cgacagagtg ggtaaaggtg attacgacat tgcgattggc aactggagtc cggattttgc
    10201 cgacccgtat atgtttatga attactggtt tgagtcagac aaaaaaggtc tgccgggtaa
    10261 ccgctcgttc tatgaaaaca gtgaggtcga taagttactg cgcaatgcgc ttgcgaccac
    10321 cgaccagacg cagcgtaccc gggactacca gcaggcacag aaaatcgtca ttgatgacgc
    10381 tgcttatgtg tacctgttcc agaaaaacta ccaactggcg atgaacaaag aggtgaaagg
    10441 ctttgtgttc aatcccatgc tggaacaggt cttcaatatc aataccatga gtaaataacg
    10501 tgaccttctg gagtatttta cgccaacgct gctgggggct ggtgctcgtg gtggcgggcg
    10561 tctgcgtgat tacgtttatt atctcgcatc tgatccctgg cgatccggcg cggttactgg
    10621 cgggtgaccg cgccagcgat gctatcgtgg aaaatattcg ccagcaactg ggactggacc
    10681 agccactgta tgtacagttt taccgctacg tcagcgacct gtttcatggt tacctgggaa
    10741 catccattcg taccgggcgt ccggtgctgg aagagttgcg tatatttttc ccggcgacgc
    10801 tggagctggc ttttggtgcc ctgctgctgg cactcctgat tggcatcccg ctgggcatac
    10861 tctctgcagt ctggcgaaat cgctggctgg atcatctggt gcgaataatg gccattaccg
    10921 gaatctccac acctgcgttc tggcttgggc tgggcgtcat tgtgctgttt tatggtcatc
    10981 tgcaaattct tcccggcggc ggaaggcttg atgactggct ggatccacca acgcacgtta
    11041 ccggctttta tctgctcgat gcgctgcttg aaggcaacgg tgaagtcttc ttcaatgcgt
    11101 tgcaacatct catcttaccg gcattaacgc tggcgttcgt tcacctggga attgtcgctc
    11161 gccagatccg ctcagcgatg ctggaacaat tgagtgaaga ctacattcgt accgcccggg
    11221 ccagcggctt gcccggctgg tatatcgttt tatgttatgc gctacccaat gcgttgatcc
    11281 catcgattac cgtattgggt ttggcgctgg gcgatttgtt gtatggcgca gtgctcaccg
    11341 aaaccgtttt tgcctggccc ggaatgggtg catgggtagt aacatcaata caggcgctcg
    11401 acttcccggc agtgatgggc tttgccgtcg tggtttcatt tgcttatgtg ctggtcaacc
    11461 tggtggtgga tttgctctat ttgtggattg atccgcgtat cggacgtgga ggtggtgaat
    11521 gatgctaagc gaggaaacgt ccgccgtacg cccacaaaaa caaacgcgat ttaacggtgc
    11581 aaaactggta tggatgctga aaggcagtcc gctcaccgtg accggcgcag tcatcattgt
    11641 attaatgcta ttgatgatga ttttttcacc gtggctggcg acgcatgatc ccaacgccat
    11701 tgatttaacc gcccgccttt tgccgccttc tgcggcgcac tggtttggca ccgatgaagt
    11761 gggacgcgat ctgttcagcc gcgtactggt cggcagtcag caatcaattc tcgccggatt
    11821 agtcgtggtt gccattgcgg gtatgattgg ttcgctactc ggatgtctat ccggtgtgct
    11881 tggcggacgc gcagacgcca tcatcatgcg catcatggac attatgctgt cgattccttc
    11941 gttggtactg acaatggcac tggctgccgc tctcgggccg agtttattta acgccatgct
    12001 ggcgattgct attgtgcgaa ttccctttta tgtgcgcctg gcgcgggggc aagcattagt
    12061 tgtacgccag tatacctatg ttcaggcggc gaaaaccttt ggtgcgtcgc gttggcatct
    12121 gatcagctgg catattttac gtaactccct gccgccgctg atcgtgcagg catcgctgga
    12181 tatcggtagc gcgattttaa tggccgccac gttgggattt attggcctgg gtgctcaaca
    12241 accgagtgct gaatggggcg cgatggtagc gaatggtcgc aactatgtac tcgatcagtg
    12301 gtggtattgc gcatttccgg gggcagcgat tttgcttacc gccgttgggt ttaatctctt
    12361 tggtgatggt attcgcgatc tgcttgaccc gaaagcagga ggaaagcagt catgacccaa
    12421 cccgttctgg acattcaaca gctgcatttg agtttccccg gttttaacgg tgatgttcac
    12481 gcgctcaaca atgtgtcctt gcagattaac cgcggcgaaa ttgtcggtct ggtgggagaa
    12541 tccggctcag gtaaatcagt caccgcaatg ctgattatgc gtctgctaca gacgggcagt
    12601 tattgcgtac atcgggggca gatttcactg ctgggagacg atgttcttaa cgctcgggaa
    12661 aaacagcttc gtcagtggcg cggcgcacga gtggcgatga tctttcagga accgatgacc
    12721 gccctcaatc cgacacgtcg aataggtctt cagatgatgg acgtgatccg ccatcatcaa
    12781 ccaataagtc gtcgggaagc cagagctaaa gcgattgccc tgctggaaga gatgcaaatc
    12841 ccggatgccg tggaagttat gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag
    12901 cgggtaatga ttgcgctggc attctcctgc gagccgcaat tgattattgc cgacgaaccg
    12961 actacggcgc tggacgtcac gctacagttg cagctactgc gtttgcttaa acataaagcc
    13021 cgcgccagtg gaactgcggt actgttcatc agccatgata tggtcgtggt gtcgcaactg
    13081 tgcgatagcg tttacgtgat gtatgccgga agcgtaattg aaagcggcgt gacggcagac
    13141 attatccatc atccccggca tccgtatacc attggtttgc tgcaatgcgc accagaacat
    13201 ggagtaccac gccagccatt acccgccatt ccagggacgg taccaaacct cacccatttg
    13261 cctgaaggct gcgcttttcg cgatcgttgc tatgcggcag gtgcacagtg tgaaaacgtc
    13321 ccggcgctga cagcgtgtgg tgacaacaac cagcgctgcg cctgttggta tccgcagcag
    13381 gaggtcataa gtgtctgaca cgttattaac gttacgcgac gtccatatca atttcccggc
    13441 ccgtaaaaac tggcttggta aaactacgga acatgttcat gccattaatg gtattgattt
    13501 acagatccgc cgtggtgaaa ccttagggat cgtcggcgag tcaggctgcg gcaaaagcac
    13561 cctcgcacag cttttaatgg gcatgctgca accgagccac gggcagtaca tccgttcagg
    13621 ctcacaacgc attatgcaga tggtgtttca ggacccgctc tcttcgctta atccgcgctt
    13681 accggtgtgg cgcatcatca cagaaccgct ctggatagct aagcgtagta gtgaacaaca
    13741 gcggcgagcg ttggcagagg agctggctgt gcaggtgggt attcgtccgg agtatctcga
    13801 ccgcctgcct catgcgttct ccggcgggca gcggcaacgt atcgccattg ccagagcact
    13861 ctcttcgcag cctgacgtga ttgtgcttga tgagccaacc tctgcgctgg atatctccgt
    13921 gcaggcgcag atcctcaatt tactggtaac gctacaggaa aatcacgggc tgacctatgt
    13981 gctgatttca cacaatgtct cggtgatacg tcatatgagc gatcgggtgg cggtgatgta
    14041 tctcgggcag attgtagaac tgggcgacgc gcaacaggtg ctaacggctc cggcacatcc
    14101 atacacccga ttattgctgg attccctccc cgccattgat aaaccgctgg aggaagaatg
    14161 ggcattacgt aaaacggatc tgccgggaaa ccgcacgttg ccgcaaggct gttttttcta
    14221 cgaacgttgc ccccttgcaa ccagcggatg tgaagtccgg caatcattag caataaggga
    14281 ggacggacgt gagctccggt gctggcgggc gctgtagaga tgctaacaac gcatcaggca
    14341 ttcacgcgcc tgacgcgtca tccggcaatg ctttacgatt tcaactggta atccagcgta
    14401 atttccgctt tcagtacctg agaaaccggg catcctgctt ttgctttctg gatgatgccg
    14461 tcaaaggtag aggcatcaat acccggcacc gcaacttcac tcttcagtgc gattttcgta
    14521 atcgcaaaac cggcatccac tttatccagc gacacatcgg cggtggtatc aatcgatgtt
    14581 ggcgtaaatc ccgcttcccc cagcattaat gaaagcgcca ttgagaaaca tgcggcatgc
    14641 gctgcgccaa tcagttcttc agggttagtt cctttttcgc cttcaaaacg cgtgttaaat
    14701 ccatacggct gttggttcag cacaccactc tcggtggata ctgttccctt cccgcgtttg
    14761 atatcgcctt cccagtgtgc ctgacctttc ttatggattg tcattgttgc tctcctgtgg
    14821 gcttaaaaag gaaattatag actacgtgag aaacagcacg aggataagca ggatattccg
    14881 aataaaaatc cggcagaaat attacttct
//
LOCUS       DAZFWP020000121        14855 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFWP020000121 DAZFWP020000000
VERSION     DAZFWP020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.121"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(23..433)
                     /locus_tag="ACHK0Z_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             complement(23..433)
                     /locus_tag="ACHK0Z_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4503549.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(732..2114)
                     /locus_tag="ACHK0Z_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             complement(732..2114)
                     /locus_tag="ACHK0Z_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU4503550.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVTAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2124..3074)
                     /locus_tag="ACHK0Z_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             complement(2124..3074)
                     /locus_tag="ACHK0Z_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU4503551.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(3217..4635)
                     /locus_tag="ACHK0Z_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             complement(3217..4635)
                     /locus_tag="ACHK0Z_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4503552.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(4635..6182)
                     /gene="fdrA"
                     /locus_tag="ACHK0Z_RS17970"
                     /old_locus_tag="pgaptmp_003594"
     CDS             complement(4635..6182)
                     /gene="fdrA"
                     /locus_tag="ACHK0Z_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4503553.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(6172..7035)
                     /locus_tag="ACHK0Z_RS17975"
                     /old_locus_tag="pgaptmp_003595"
     CDS             complement(6172..7035)
                     /locus_tag="ACHK0Z_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4503554.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(7075..7680)
                     /locus_tag="ACHK0Z_RS17980"
                     /old_locus_tag="pgaptmp_003596"
     CDS             complement(7075..7680)
                     /locus_tag="ACHK0Z_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU4503555.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7938..8435
                     /locus_tag="ACHK0Z_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             7938..8435
                     /locus_tag="ACHK0Z_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4503556.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGSKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            8527..9459
                     /locus_tag="ACHK0Z_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             8527..9459
                     /locus_tag="ACHK0Z_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4503557.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9501..10589)
                     /locus_tag="ACHK0Z_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             complement(9501..10589)
                     /locus_tag="ACHK0Z_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4503558.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(10731..14714)
                     /gene="ehaA"
                     /locus_tag="ACHK0Z_RS18000"
                     /old_locus_tag="pgaptmp_003600"
     CDS             complement(10731..14714)
                     /gene="ehaA"
                     /locus_tag="ACHK0Z_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU4503559.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKDQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANRGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGNQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         3401 a         4043 c         3522 g         3889 t
ORIGIN      
        1 tttcgcgtag ataaatagtt atctacacaa cgtcaataag ggcatattct tcaaatataa
       61 cgtccatttc tggtgaatag cttccttctg cctggaaaaa acgacggtgt tcattacgcc
      121 aatattcaag actgaggtca ccttcacctt ccttacgggc aagctccacg gtgacatcag
      181 aaaagcgcag taaatgcagg ccaatcactc tgataacaca tacaggctca ccacggctat
      241 tttctacaat cttatactca ccaatcatcg gaaatgcgtt gtcttcaata catcccgcca
      301 gcgaaccaca ggaagctgtt ttaacaccag tcgcaatcaa cgttgcgagg tgatcggcca
      361 acgctgctga atcgccaaaa gtacaaaagg aagcatgggg atatttttgt gagaattttt
      421 cttttatggt catggcaata tttttgcgat tacggaaaaa caaatcacat cttaattact
      481 gttggtaacc atcagcaaga gtatcattgg tttttcgata ctgaatcttt tttatccgaa
      541 gcaggtcaca tttatggcct gataaatacc tttgttaaaa ctcacttttg tgtatttctc
      601 tcaaataatc ccctggaaat tatgccgcga gattataaag ttcaggatca ttcattagca
      661 aaccattccc gccccactcc ctggggctta aatctcttac aattcaccgt tgcaggcgtt
      721 tcaaatgcgc atcaacctgc cacactcccc cacaccagtt gccctttatg gaacgttgcg
      781 gttctcggcg agatacgcgc caccgcctcg gcggaacagg aggcgtccac cagcacaaag
      841 ctggcatcat cctgcgcttt tggccataca cgctcgcctt tttcattcag cggcaataca
      901 tcgccagtgg ctagaaacag cgaacgggag aggttctgtt cgttaggacg aatatagagc
      961 tgcgcgtaga gattggcttt ttccagcatg tcgcccagac cataaggcga ccagtggtcg
     1021 ataacgctgt cagtgccagt catcactttt acgcctttgt cgtgcaactg tttgagcggc
     1081 atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa tttgctgcgc cgccatccga
     1141 ttcgccagcg catcgacctg ttgttcgttg agcgtagcca gcgcaaaggc gtggctgatg
     1201 gtcagcttac ctttcagttg cggcgttttc tctaccgttt caaccatata attgatggct
     1261 gtcacgcccg acggagtggt ttcgtgcagg tgaatatcga cacctttgtc gtagtccagc
     1321 gcaatctgaa acatggtgtc gagggatttt tccatcgcgc catcgacact ggtcgggtcc
     1381 agcccgccga cgtaatgcgc acccgcctgc atcgcttcgc gcattaaggg ttccgatttc
     1441 gacagcagca aaccgtgctg cgggaaggcg acgatttcac actcgaagcc cgcctgacgt
     1501 cgcgccagca ccgcctgcaa attctgcaga tttttcaggc cggaaaccgg ttcgatattg
     1561 cagtggctgc gggcaatggt ggtgcctttc gactgcaata gatcgatcag tttttctgcc
     1621 cgttcctgcg tgtacggttg cagttccggc agcattttct gctcaagctt gatcatgtcc
     1681 tggatagtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta gaaggttttg
     1741 tcgagatgaa tatgcatgtc gcgagtggtg ggtagcatca acttaccgcc agcgtcatag
     1801 tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcgacaat tttgccgtcc
     1861 tgaatctcca cggtctggcg cgccgtgcgg gtctgcaccg ccacaccgtt ttcgtagtca
     1921 aaaccggttt ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat tttcatggtg
     1981 ttgttcgctt cgcagtttag ggtgccagct accgccgcat gggcgagcgg cacagaggta
     2041 ccaaacagcg cggcggcggt gaccatctta ccgctctggc tcagaaattc acggcggcta
     2101 ttgctttctt tcatcctacg tccttacgtt ttaataatat gggtgcccgt ttcaccgcgc
     2161 agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc ctgctccagg
     2221 aacgtcaggc tggcgatgat ttttggcaac atgctgccgg gcggaaaatg cccttcctgc
     2281 atatagcgag tcatggtggc aatatccacc cgatccagcg cctgctgctg cggtttacca
     2341 aagtggatac agactttttc gacgccggtg gtgatcacca gaatgtcggc gtgaatttca
     2401 cgggccagca gcgcggtaga gagatctttg tcaataaccg cgtccacgct ttggtaatcc
     2461 cccgcttcag tacgcactac cggaattcca ccgccgcccg cgccaattac gacaaaacct
     2521 tgttggatca gcgccttaat ggcgggtgct tcgacaatac gtttcggttc cggcgaggcg
     2581 accacgcggc gatagccccg tccggcatct tcaacaaaac accagtcagg gtttgccttt
     2641 tgtagtttgt cacgctggct ttcactgaag aatgcaccaa tgggcttggt gggatgggca
     2701 aaacctggat cgtttttatc cacttccacc tgagttacca cggtgacggc ttttttctcg
     2761 ccgtgacgcg ccagccggtt attcagcgcc tgttggatca gatagccgat gccgccctgc
     2821 gtatctgcca cacagttcgc cagcggcgtt aagggcagcc cttcgcgctc gtgggcaatc
     2881 tccgcacggc gtaaatccag cccgacctgc ggcccgttac cgtgggtcag cacaatgtcg
     2941 taatcggaag ccagcatttc cagcaccgta tcggccacgg ctttcaccgc ctccgcctga
     3001 tgctcaatcg actggctggc gttatctttg ataatgctgt tgccaccaat ggcaacgacc
     3061 acaagctctt tcatgttgtt atgtccgcta cagagggtga ttaggttatg accaacgcca
     3121 gactgcctga tgcgctacgc ttatcaggcc tacaattctc gggccatccg taggccggat
     3181 aaggcgttca caccgcatcc ggcataaaca tgtaagtcag acgccgtaac gttcgcacca
     3241 gccgagaatg gctttctcga agcaggcgaa tggtggatgc acaatgcccg cgccaatcat
     3301 gccgacgccc gcatctttat gggcgatggc ggtgttgatc accggcagaa tgccgctgct
     3361 acccacgcgg gtgatgtcga ttgccgacgg cacgcccata aaaccgagca gcggaatggt
     3421 gacgttgggg ttttcgccaa gggtgatttc gcgcatctga cgggagaaat cgatagcctc
     3481 ttccaccgtg ccgcccacca gtgcgacgat tgcaggcgcg gtcgccatgg caaacccgcc
     3541 aataccgtag gtttctgtga tggcgctgtc gccgatatcc agccccgaat cttccggctt
     3601 ataaccagca aacatcgggc cgatgacctg ctgcgctggg ccggtaaacc actgccccgg
     3661 caggccgctg acccgcaggc cgaactcgac gccgttacgc gccatggtgg tgaccacggt
     3721 gctgtattcg atgccgtgcg ccgcatccat cgccgcttta cacatcgcca tccacgtcgg
     3781 gccggagaag tagtcgctgc tggcgacaaa ctcgaacact tcgcgctgtt gctcgacgga
     3841 atagcctgcc tgaatgatcc ccggcgtcag tgcctgaatc agcagcgtcg tcccggcgtt
     3901 attgcggtta tggcactcat cgcccatatg cagcgcctgc gccagcatta agcgcagatc
     3961 gatctcaccg ataattttca tcgcgtcgcg cagcattggc ccctgcacat cgcgcatcca
     4021 gttgaggcgg tcgatcacgc tctgatcgtt ggcacccata cgcaaaatct tcgccatctg
     4081 ctcgctcata ttggtataag cgatgttgcc gtaggttttg tttttcacga tgtgcataaa
     4141 catcgaggcc gaggtaacgc ccgccatcga tcccacgcag tcgtgttcgt ggcacggcga
     4201 gaaggtgatc tccccggagg ccgccagttc agtcgcctca tcgagatctt tcgccagccc
     4261 ttcgaacacc aacgccccgg tgaccgcgcc tttcatcgcg ccgcacattt tttcccaggt
     4321 gatcggcggc ccggcgtgaa gaatggtttt cggcgtcatg cccggcacca cgttaatcgc
     4381 ctgatcaaaa ccaatcagca ccggatgcga ctggataatg cgctccagcg cctgctggtt
     4441 agcggcggcg attttgtctg ccagcggcga atcggcaatg ttatccagcg cctgcaccac
     4501 ctgcatattg ccctgccccg gcggcgtcca gtcgagttga gtcacttcta catgctgctt
     4561 tttcaggtca tcgctaaaca tggcgatacc gacgttaata acgttcaacg gttggctaaa
     4621 cagtgactgg ctcatcaggc ttcctcccct ttgcagataa attcacgcgc cagcaatccg
     4681 gtattggtgc tgctgctcgc cagaatcact ccggcatcaa gcagcatctg gctttgttgt
     4741 tctaatgatg gcgtatcgag atcggtgccc agcacatagg cgagaatgat caactcgcgt
     4801 ccttcggcgg cggcgatcgc tttcgcttct ttgatcgcct cgatggtgga gccgaccgga
     4861 tcttcatgcg atccaaatcc gagcacaaaa tccatcacga tcaccgccac ttctggatcg
     4921 cgcgcctctt cgatcaagcg actgatgcgg ttggtggggt caatcattgg atgcggcttg
     4981 ccgttggtga agtcgtcatc gccaaagtcg aggaaggtgt gtttgatgct acggttgata
     5041 tcttgcaggc ggaattccgg atcgggctga atgttgctgt agacatcgcc gtgtttttcc
     5101 atcaccgcga acatggtttc gtcacacagc gtgccgccac agaacaggcc acgaatgtac
     5161 ttctgctgcg gttgcaggcg agcacgtacg tcagcaatca acggctggtt cagcgtatgc
     5221 aggtcgagat gttcctgttt cacgccggag agcatcaccg ctttcaatgc cgcctctttg
     5281 ctgccgcggg caaactgtag cccctgctca tccactggcg tttcgccacg accgaggaaa
     5341 cagaccacca ccggcttgcg gcaggcgcgc gcgcgttcca gcactttgcg ggccaccgca
     5401 ggcgcgggcg gtttggagat aagcacaatg atttcagttt gcggatcgtt ttccagcatg
     5461 ccgatggcgt cgagcatcat caggccgccg attttctcgc tcaggtcgcg cccgccggtg
     5521 ccaatcagtt gcgaaacgcc gccgccaaat tcatgaatgc ggacgctcag ctcctggctg
     5581 ccggtgccgg atgcgccaac aataccgatg ttgccgcgac gcacggcgtt accaaaacag
     5641 agcgccgcgc cgttaataat cgccgtgcca cagtctggcc ccatcatcag cagccctttt
     5701 tcgtgggcca gttgcttgag cgccaattca tcttcaactg agacgttatc ggaaaacagc
     5761 atcacgttga gatcgttttg cagcgcctga cgcgcttcgc gagcggcaaa cagaccgttg
     5821 accgaaatca ccgccaggtt actttccggg atatgctttt tggcgctggc aatagtggcg
     5881 taacgcgcct catgcgagcc gctttgcgct ttggtgttga acagttcttc aatctccacc
     5941 agcaactgtt cgttgtccgc gcccgctttg ccgttgatga caatcatcag gtcgccgttt
     6001 ttcgcctgtt ccagctccgg cgtcagcagt cctaaatttt tcagtacacc tttgttcatt
     6061 tcggtcgcca tcgccacaaa tgcctgctcg acgccgtcga gtttatttgc acgcgtggag
     6121 atagacatca gcgagacaga atcaaaatag gtattcggtt taatgactat tttaactgac
     6181 ataattccct ccgtaggttt ggctcagcgc acaaccatcg gccatgccat acagcatgtc
     6241 gcagccggaa ctggagccaa tatgtttaat tttttctatt gcctgagttg cgttcccagg
     6301 gatgtcataa ataatgttgt gaataaaacg atgaatattt tcccggcaac gttgttgtaa
     6361 tgcggcttcc agcgttatag cacttaataa tgtggtgtta tttctgccgc gctgcatacc
     6421 gagataaaat tcctctttgt atttttccga cggatgcccg ggaataaata aaataagcgc
     6481 cagacctgtt aaataatcgt cggctgatgg cgtaaggcca atccctaacc caatcatact
     6541 ggcgaccgct gcggcgatat tttgtttttc gtttactgcc tgaataactg cccgtcgttt
     6601 aatatgtagt tctttattta acgcctgata aaacggatta tcgccgcgat aattaaacag
     6661 ggtttcattt tcccccaggt gctgatgaat aatgtcgtgc cactgttgcc aggcgataag
     6721 gtgaaaattc acagcggtca tttgcaaggt tggggacagc caacgccgac aacgactcat
     6781 ctctatatga agatgttgac caaccgtaat accttgatcg tcaaactgaa ccttatcacc
     6841 atgccggaac agatcatcaa agtgagtgag tgccaaccga caactgtttg gcgcattgtc
     6901 gtactcttca caaagcagcg tcagcaactg ctggcgagcc gggagtgcga tatttaccgc
     6961 tctggcaaag acctgttcaa cctgtccttg cccccgctgc gccagaaaat ccgcatccgc
     7021 agttagcgcc cacacacacc gtctttctgg tgtgttgata gcaagcctcc cggtttatgc
     7081 acctgcggca atcagtaact gcgcaatctc ttcaaagccc cgttcccgcg ccagttccag
     7141 tggcgttttg ccatatttat cggtcagatg cgggctggca ccgtgttcca gcaataactg
     7201 cacaatcgcc tgctgtttaa taccaccatc attaagcaca atcgcttcca gtagcggcgt
     7261 ccagcctaca tggttggtct ggttaacgtt aatctccgta tgcgccaaaa gctcttttac
     7321 aatacttaaa tggccttttt cacaggcagg cgtcaggccg acaccgccaa aacgggttac
     7381 gcaattaaga tcgggtttag ccggtaaaat aattcgtagt agcgttaaat catcgttcag
     7441 acagctaatt aaaaaaggat ttaaacaggt atgatcttgc ttattaatat ccgctccggc
     7501 atcaattaat gcctgaacgc aagcatattg ctgatataaa cttgccagcg taattgccgt
     7561 tttcccctga cgatcgcagg tattaatatc gacacccagc gcaaggcagg tttttacttt
     7621 atcgatatca ccctgttgtg cagccaataa atagtcggca ggtagatttt ttatactcat
     7681 tcacattccc ctggtttttg tggctgtttt cgatgaattg agaatagcaa cgataatctg
     7741 ataaaagaac ccgcttaaat ttaattcgct gtatttcgcc tttttgttga atgatgttca
     7801 acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc tttgacccga taccctttca
     7861 ctcaatcagg ttttatttct ctatagtctg cacatgtaag agtagctttc gtccatgtct
     7921 aaccagaggg gaacaagatg aacggactca cggcaacggg tgtcaccgtc ggtatttgtg
     7981 cagggctttg gcagttagtg tcttcacatg tcgggctctc tcagggatgg gaattattag
     8041 gaaccattgg cttcgtcgcc ttttgcagtt tttatgccgc aggcggcagt aaatcggggt
     8101 ttatcagaag tctggcggtt aattattccg ggatggtgtg ggcatttttc gccgcgctgg
     8161 ctgccggttg gctggcctct gtaagtggac tgtccgcgtt ctgggcgagc gtaattacta
     8221 ccgtaccctt ctcggcggta gtggtctggc aagggcgttt ctggttactg tcctttattc
     8281 ccggtggctt tctcggcatg acgctgtttt ttgccagcgg gatgaactgg acggtgacgt
     8341 tactgggttt tctggcgggt aactgcgtgg ggataatttc agaatatggt ggacaaaaat
     8401 taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat gattatctat
     8461 ctttaaccga tacctctaca gtcatagctg gagaaattca tagtagataa tggcgagaat
     8521 ggtttaatga actcaatttt taccgaagag aatctgttgg catttactac cgctgcacgt
     8581 tttggcagct tcagtaaagc cgcagaagag ttgggtttaa ccacttccgc cattagctac
     8641 accatcaagc gtatggagac ggggctggat gtggtgctgt tcactcgcag tacccgcagc
     8701 attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgacct gctgaatgat
     8761 ttttatgcca tcaaacgcag tatcgatact atttcgcagg gcatagaagc gcgtgtgcgc
     8821 atttgtatta atcagctgct ttatacgccc aaacacaccg cacgcttgtt acaggtgctg
     8881 aaaaagcagt ttcctacctg ccagattacc gtcaccaccg aagtgtataa cggcgtctgg
     8941 gatgcgatta ttaataatca ggccaatatc gccatcggcg cacccgatac actactggac
     9001 ggcggcggta ttgattacac tgaaattggc gcgattcgct gggcttttgc tatcgcgccg
     9061 gatcacccgt tagcctttgt gccggaaccc atcgccgaaa gccaactgcg tctctaccct
     9121 aatattatgg tggaggacac cgcgcatacg attaataaaa aagtgggctg gttgctgcac
     9181 gggcaggagt cgattctggt gccagatttc aacaccaaat gtcagtgcca gattttaggt
     9241 gaaggaattg gttttttgcc ggattacatg gtccgtgagg cgatgacgca atccctgctg
     9301 gttacccgac aaatccataa tccgcgccag gattcgcgca tgttactggc gactcagcat
     9361 tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat ttgcgccaaa tggcatatta
     9421 acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac ctcccgatgc
     9481 gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga gataaattta
     9541 ttacccggta ctggcggaga gtacaaatag ccctgcaaaa agtgaacgcc tttaccgatc
     9601 attaaatccg cctgctcctg ggtttctacc ccttccgcca cgatactcag accaggctta
     9661 cgcgctagtt cgacaatatt gtccacaata tgaccggaga tttcgtcgac actcgccatt
     9721 tgcacaaatg acttatcgat cttaataaaa tcgaccggga acgcctgcaa gtaacgatag
     9781 gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg atgaaggctg
     9841 tcaaatatcg ctctggcttc tggcgttacc ggaatagggt tacgttccgt tagctcgaga
     9901 accagcttga ttttatcgtt acctaatttc ttaaccaggt tcagacactc tttttcaaat
     9961 cccgctgcca aaaaacaacc cgccgagacg ttgatgccaa tatggaaatt gtccggcagc
    10021 aaatgtttta ccggcatcag aatatccgca gtctgtttca tcagttggcg ggtcattatg
    10081 acaataagac cggatgactc cgccagagga ataaactgat ccggtgggat aattcccgtt
    10141 tgtggatgtt cccagcggac aagtacctca cagcccgtca gtaccccagt ctgcgcgcag
    10201 aaaaccggtt ggatccacgg tttgaattcg tggttttcca acgccagact gatggcttca
    10261 ggcgtgacga tatggtgaca acggttatca gggatataaa aattattttt atttcccgtg
    10321 ccactgatga cttgcggatg atattgaaaa aagatatccc gccagaaggt aatatcactc
    10381 tggatgccta gtttttcata cagctgcttc ttttgatgtg aaattgtctt tgcactgcga
    10441 tttctgtact gtgagatttc tgtaaccgac attccctcca ttagcagctt gagtaaaagc
    10501 cgttcttttt cggacacgat accaggcaac gtcaaattca ccgttgcccc gaactcttcc
    10561 aaaaaaacac tcaaatcatg cgaagccatt gaacacacct tgattaaatc ttgtgcgcca
    10621 gctaaatctg cgagtcttca ctctgactga taaagctggc tgcaagaaaa tgacacagag
    10681 agccgaatta tctcccgttc ggctctctct ccttttactc attattcaga tcagaattgc
    10741 cacttaatgc caaccattgc agaggtgtca ttatatcccc tatcggcaac ctgaacgccc
    10801 acattccccc agacattcag gttggcattt agctgtcctt ccaccccggt ttttatctca
    10861 gcaatatttc gggctccatc ctgagtgacg gacacgccat ccatactggt gctgaaatcc
    10921 ttactgttat gcagccagtt cacttctaca aacggctgga actcgcggga tttaccgtca
    10981 tccattttat ggtggctctt cagccaggtt tttacgccaa gtcgggtttg aacattgcca
    11041 tcaccgttgc tatgaacgag ggttccgttg ctttcgcggt gcttatcggc tttgactccc
    11101 atccaggtaa cctgtgcttg cggctgaaca taccattcat tacgcgttcc ctggttgcca
    11161 ttaaattcag ctaatttgtg tttgtatcca gcttccagtg aagcggtaaa tccttttgat
    11221 ttataggatt cactttgtaa gtcatcccct ttcactgtgt tatcaaacca gctgtactgc
    11281 gcccaactgt cgagatacgc gccattacgc gactcgtcat cggcatacca ggtggcatag
    11341 aggccggtgc tatatccgtt cacactcgct tttgcacgat aaccggttcg cgaggaaatg
    11401 gttttgctgt cgctgttgcc atatcccgcc atgaccccaa catgccagct gtcgctgccg
    11461 ttttggctcc actgcgcgac atcgcctccc agttgcagaa catagcgatt gctttgggtt
    11521 ttcagctggc cgctgccatc acgccattta ttatgaccac cttcatggcg catccacata
    11581 gtggtttgtt tctgctcacc cgtcaccatg tcggtatagt acgtattacc cagacgctca
    11641 tgcagacgcg tggtgaacat tgtattcgct gctgcaaggt tcgcaatgta gctacccgcc
    11701 tccggtcgca ggtcattatc cacattcaga tccggacccg gcgtaggtgt cgggttaggg
    11761 ttcggctctg gattcgggtt tggctccgga ttcggcatcg ggtctggctc cggctgcagt
    11821 tcaggagaat cactgccgct ggtcagatac cagttaccac gatttgcccc ttgtccacgc
    11881 gcgagagtgt agtcatacgc ccccgcaacg atacgaccag cctgaacaaa ttcgccctca
    11941 gacttaccgt ctacatggat aagttcgata ccgttaaggg ttttcgcacc tgtaccgcca
    12001 gcgttattca ccgtaacggc agtcgtgccg ctagtatcgc cctcgacaac caatttatcg
    12061 gttactgagt catcgccatt cagtaccgta ttcattacta gttgaccgtt attaccgtgg
    12121 tagttcccct tcaccactaa cacattgcca gcttgctgtc ctgacattcc ggtcatcagc
    12181 gtaccgttgt tggtcagatt gccgcccagg gtgtaattca cagacgcagc cgctcttttt
    12241 tcagcgctat tattcaacgc cagggtaccg ttgttctcaa cgtttgcgct acccaatccc
    12301 tgcgcatgac ttaccaggac attagccgaa ttagcaatgt tcacattcgc cgtcaggttg
    12361 ctgttatcgc ccgcgatgtc cagcgtgccg ccctgaatat tcagctctcc gctccccgtt
    12421 aacgaaccgg ttgaagtgcc cccgttggta accgtcagac tgccgccatt taagctcagc
    12481 agtgaatcgg cgctgccatt gagcgtgccc acggtctggc tgtgaccatt catatccagt
    12541 tcagtctctg ccgccagacg cagttcatgg gtattaccca acacgttatc gttattcaac
    12601 aaaagcgtcc cactgcgcag atcggtaaca ccggtatagt cgttgtcttt gttagaaagc
    12661 gatacggtct gatccttctg gctgctgaat gccaggtcac cgctgccggt aatctttgcg
    12721 ctgagatcgg cggcattctc gcttttaccg ttagagctca gcaccagagc atcgctatcg
    12781 gtagcgtgta aatcaagctg ggtcaggccg taaccgatat acagaccgtc attgtttacc
    12841 ccactcgtca gacgatagtc gtaagtgccg acggccgctt tttgcccatt ctgctgaata
    12901 tcacgttggg cactgctgga gaggatttgc ccatttttat cctgcaattg cagattgccc
    12961 gcatcgccca gaacttccgc accttgcgca tcaaccaact taatggtgct gttaccatca
    13021 tcgacttcag tgagcgataa cgcagagtca atatcacgag atactgagct tacaacgtca
    13081 ctgtcagaaa cctgaatcac accctcgccg cgcaaatcca gcgttttact aacgttgacc
    13141 gtcccttctg tcatctgtgc acctgctgtc agggcaccaa aatcaacggt tccgccggag
    13201 aatcccaaag tgctaatgga gtgagcgccc ttacccactg ttaccaggct tccctgaccc
    13261 gcagtgatcc cgcctctctg cagaaactca gcattctttt ccaggttaaa tgtcgtgttc
    13321 tggaacgcca gtgaaccctg gaaccagtct gtataagcaa actcatcgaa ggagaattcg
    13381 tgattcccca tgtttatttc aacaatacct ctaccttgaa gatcgttatc aaatacccaa
    13441 tctttattgg cattgatttg cagtttgcca tcaacatcca ggtccgcacg gccgatgttc
    13501 ttgtcttcag aaataaccgc gacagcacca gaagcgacat caatatatcc gctgaatgca
    13561 tagttattgc cggtcagtgc cactgtccca ctgtcgatac ttattgtacc attgccggaa
    13621 atcttattgg caaaggtcat gctatcgtca gccttcaggt gtaatttccc gcggttgttg
    13681 atctctgctg tacctatatt tttggtgatc cctaaagcgc cattttgctc aattttgact
    13741 ttaccactaa aggcagagtt atcaccggtt aagtaagtcg tgccattgag cgcattgata
    13801 tagcccaggc cctcgctgcc actgacaata tccgcggaaa tagtcgcgtt tttatccgtg
    13861 tggtttaagg tgattttctt atcggatgtc tttgcccaca caaactcaat tttttcggca
    13921 tcaataatcc ctgcagcctt cgcagcactt ccttcctgtg cacctaacgc taactcagag
    13981 ttggtgctta aactaatttt cggtgcggat attttacctc catctgatac gactaatgtt
    14041 gtatcagaac gttggtcgta ataacccagg ctaatatgtt cacgagcatt aacttcacca
    14101 ccgttattaa ccgacaggaa ctgattaccg ccatatccgc cgtaaacgcc cataaagaag
    14161 gtccctgcat ttacctgact accttcgccg cttacattcc cctgcgtata agtcagacga
    14221 ctggtgccct taacatgaat tttgccgcca ttggagacat taaaatagcc ttgatcatgt
    14281 ctcatgcctg agtttgactc atacaaatca tcagcatata actcgctgcc agcattggag
    14341 acattcagga accccgtacc tttcagcaaa aagttaagcg ggccattgat tgttaccttt
    14401 cctccggtaa tatccaaccc accaccagaa gcgagagtca gacgcggacg ggaagtatct
    14461 gtcgtattga ttaccagatt ggcattgtca gtcaccgtaa tacggtaatc gatgctctgg
    14521 ttctctttgc caaaatcaat agctttatcc tggtttacga tgacatcatc ggcgaatacc
    14581 agagatgaat ataaacttat cgcgctaatt aaaacagccc gggacttttt tccttttact
    14641 ctgctcccca gctccgagca gacgctccaa cagttacggg acctgttcca ctttagccga
    14701 tatattttat tcatcacaat ctccagcaat acatccaaat aaaaccaaga ttgcataaac
    14761 agcaacgcat taaatgccat tctggttact gttcatcgca tataaaaatt caaaaatttt
    14821 gcatagcatt ggcaaccaca tatctgagta tgtag
//
LOCUS       DAZFWP020000122        14478 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFWP020000122 DAZFWP020000000
VERSION     DAZFWP020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.122"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            27..593
                     /locus_tag="ACHK0Z_RS18005"
                     /old_locus_tag="pgaptmp_003601"
     CDS             27..593
                     /locus_tag="ACHK0Z_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503560.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            644..3103
                     /gene="ybgQ"
                     /locus_tag="ACHK0Z_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /pseudo
     CDS             644..3103
                     /gene="ybgQ"
                     /locus_tag="ACHK0Z_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3118..3849
                     /gene="ybgP"
                     /locus_tag="ACHK0Z_RS18015"
                     /old_locus_tag="pgaptmp_003603"
     CDS             3118..3849
                     /gene="ybgP"
                     /locus_tag="ACHK0Z_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4503561.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3846..4907
                     /gene="ybgO"
                     /locus_tag="ACHK0Z_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             3846..4907
                     /gene="ybgO"
                     /locus_tag="ACHK0Z_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503562.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5059..6105
                     /gene="abrB"
                     /locus_tag="ACHK0Z_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             5059..6105
                     /gene="abrB"
                     /locus_tag="ACHK0Z_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4503563.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6102..6893)
                     /gene="nei"
                     /locus_tag="ACHK0Z_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             complement(6102..6893)
                     /gene="nei"
                     /locus_tag="ACHK0Z_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGU4503564.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6929..7663)
                     /gene="pxpA"
                     /locus_tag="ACHK0Z_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             complement(6929..7663)
                     /gene="pxpA"
                     /locus_tag="ACHK0Z_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU4503565.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7653..8585)
                     /gene="pxpC"
                     /locus_tag="ACHK0Z_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             complement(7653..8585)
                     /gene="pxpC"
                     /locus_tag="ACHK0Z_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU4503566.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8579..9235)
                     /gene="pxpB"
                     /locus_tag="ACHK0Z_RS18045"
                     /old_locus_tag="pgaptmp_003609"
     CDS             complement(8579..9235)
                     /gene="pxpB"
                     /locus_tag="ACHK0Z_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU4503567.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9258..10001)
                     /gene="ybgI"
                     /locus_tag="ACHK0Z_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             complement(9258..10001)
                     /gene="ybgI"
                     /locus_tag="ACHK0Z_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU4503568.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10272..11753
                     /gene="dtpD"
                     /locus_tag="ACHK0Z_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             10272..11753
                     /gene="dtpD"
                     /locus_tag="ACHK0Z_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU4503569.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11795..13213)
                     /gene="phrB"
                     /locus_tag="ACHK0Z_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             complement(11795..13213)
                     /gene="phrB"
                     /locus_tag="ACHK0Z_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU4503570.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13210..13719)
                     /gene="ybgA"
                     /locus_tag="ACHK0Z_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             complement(13210..13719)
                     /gene="ybgA"
                     /locus_tag="ACHK0Z_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU4503571.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13870..14478)
                     /locus_tag="ACHK0Z_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /pseudo
     CDS             complement(13870..14478)
                     /locus_tag="ACHK0Z_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3499 a         3868 c         3500 g         3611 t
ORIGIN      
        1 tttcttttct gttaaaggac tccgttatgt ttaaaggaca aaaaacattg gccgcactgg
       61 ccgtatctct gctgttcaca gcacctgttt atgcggctga tgaaggatcc ggtgaaattc
      121 actttaaagg tgaagttatt gaagcaccgt gtgaaataca tcaggatgat attgataaag
      181 aggttgaact cggtcaggtg accaccagcc acattaatca gtcacatcac agcgatgccg
      241 ttgctgtcga cctgcgctta gtcaactgtg atctggaaaa ctccagcaac ggttccggtg
      301 gcaagatttc taaagttgca gtcacctttg atagctcagc gaaaaccacc ggcgcagatc
      361 cgattctcaa caacaccagc acgggtgaag ccaccggcgt cggcgtacgt ttaatgaata
      421 aagatcaaag caatatcgtt ttaggcaccg ctactccaga tatcgacctg gctccgacct
      481 ccagcgaaca aacgctgaat ttctttgcct ggatggaaca aattgatcag gctacacctg
      541 taacgccagg cgcagttacg gcgaatgcaa cctacgtgct ggattataaa taagtattac
      601 tttggcggcg ctatcgccgc ctttcataaa atatttatca ggtatggaca ccgtgaatat
      661 ttatcgactc tcttttgtat cctgcctggt cgtggcgatg ccttgcgcat tggcggtcga
      721 attcaacctt aatgttctcg ataaatcaat gcgcgaccgc attgatattt cattattaaa
      781 ggaaaaagga gtcattgctc ccggtgagta ttttgttagc gttgcggtaa ataataacca
      841 aatcagtaac gggcaaaaga ttaactggca caaaaatgac gataaaacca ttccgtgcat
      901 caatgattta ctggtcgata aatttggctt aaaacctgaa gtccgtcagt cgttaccatt
      961 gataaatcag tgcgtcgatt ttagctcccg acctgaaatg ctcttcaatt tcgatcaagc
     1021 caatcagcaa ctaaatatca ccattccgca agcctggctg gcgtggcact cagaaaactg
     1081 aaccccaccc tccacatgga aagaaggtgt cgccggtgtc ctgatggatt acaacttgtt
     1141 tgccagcagc taccgcccac aggatggcag cagcagcact aatcttaacg cctatggaac
     1201 agccggaatc aacacggggg catggcgctt acgcagtgat tatcagttga atcagactga
     1261 tagcgatgat aaccatgaac agtcaggcga aatatcacgt acctatcttt ttcgcccgtt
     1321 accgcaatta ggttcaaaat taaccctcgg cgaaacggat tttagttcca atattttcga
     1381 cggtttttct tataccggcg cggcactggc aagtgacgat cgaatgttgc catgggaact
     1441 acgcggctac gccccacaaa ttagcggtat tgcacagacc aatgccacgg tgacgatcag
     1501 tcaatcaggc cgcgtcattt accagaaaaa agtcccacca ggcccattta tcattgacga
     1561 ccttaatcag tctgttcagg gcacactgga tgtcaaagtg acggaagaag atggtcgggt
     1621 gaacaatttc caggtttcgg cagcatcgac gcccttcctg actcgtcagg gacaggttcg
     1681 ctataaactg gccgcgggtc agccacggcc ctccatgtca catcaaactg aaaatgaaac
     1741 cttttttagc aatgaagttt cctgggggat gctgtcaaac acctcgctgt acggcggcct
     1801 gctgctttct ggtgatgact accattctgc cgcaatgggt attgggcaaa atatgctgtg
     1861 gcttggtgcg ctgtcgtttg atgtcacgtg ggccagtagc cattttgata ctcagcagga
     1921 cgagcggggc ttaagctacc gttttaatta cagcaaacaa gtggatgcca ctaacagcac
     1981 gatttccctc gccgcttatc gtttctccga tcgtcatttt cacagctacg ccaactatct
     2041 ggatcacaaa tacaacgaca gcgatgcgca ggacgaaaaa cagacgatca gcttatctgt
     2101 gggccaaccg attaccccac taaacctcaa tctttacgcc aacctgctac atcaaacctg
     2161 gtggaatgca gacgcctcca cgaccgccaa catcacggca ggttttaatg ttgatattgg
     2221 tgactggaga gatatctcta tttcgacgtc attcaataca acccattacg aagataaaga
     2281 tcgcgacaac cagatttacc tgtcgatttc gctccccttc ggtaacggtg gtcgggttgg
     2341 ctatgacatg caaaacagta gccacagcac cacacaccgc atgtcgtgga acgatacgct
     2401 ggatgaacgt aatagctggg gcatgtctgc cggactgcaa tccgaccgtc ctgacaatgg
     2461 agcccaggtg agcggtaact atcagcacct gagttcagcg ggtgagtggg atatttctgg
     2521 tacctatgcc gccaatgatt acagttccgt cagcagcagc tggagcggtt ctttcaccgc
     2581 aacccaatat ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac gcctgatggt
     2641 cagcaccgat ggcgtggcag atattccggt tcagggcaat ctcgactaca ccaaccattt
     2701 tggcattgcg gtggtgccgt tgatttccag ttaccagcct tccaccgtgg cggtgaacat
     2761 gaatgactta cccgacggcg taacagttgc agaaaacgtt atcaaagaaa cgtggattga
     2821 aggcgcgata ggttacaaat cactggcttc ccgttccggt aaagacgtta acgtcatcat
     2881 tcgcaacgcc agcggtcagt ttcctcccct cggagcggat atccgccagg atgacagcgg
     2941 cattagcgtg gggatggttg gcgaggaagg acatgcctgg ttaagcggag tcgctgaaaa
     3001 tcaaaagttt accgtggtct ggggtgatag ccagcattgc tcgctccatc ttcctgaaca
     3061 tatggaagac accgcaaatc gcctgatttt accttgtcat taattaagga aatgaaaatg
     3121 acatttatga aaggactacc ccttttgttg ttggttgcca gtctgtgcag ccacgctgca
     3181 ctacaacccg atcgcactcg tattgtgttt aacgctaatg ataaagctac cagtctgcgg
     3241 gtggataatc gtagcgataa actcccctac ctcgcttatt cctggctcga aaatgaaaaa
     3301 ggggagaaaa gcgatgatct tctggtcgcc ctgccgccga tacaacgtct ggaaccgaaa
     3361 gcaacaacgc aggtacggat agtaaagcaa gcctcgacca caaaattacc gggcgatcgc
     3421 gaaacgctat ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa aaacagcgac
     3481 catgccgttc tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg accggctgca
     3541 ttacgcaaga aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca acagggcaac
     3601 caactgacac tgaaaaatcc taccgcgtat tatctgacca ttgcctacct gggacgaaat
     3661 gaaaaaggcg ttctccccgg cttcaaaacc gtgatggttg cgccttttag tacggtcaac
     3721 acaaacaccg gaaattatag cggcagccag ttttatctcg gttatatgga tgattacggc
     3781 gcgttacgca tgacgacgct caactgcagc ggacaatgcc atttacaagc cgtggaggcg
     3841 aagaaatgag tgctggcaag ggagtgttgc tcgtcatctg tctgttattt ctgccgttga
     3901 agtctgcgct tgccctgaac tgctactttg gttcatccgg tggctcggta gaaaaatcag
     3961 aagcgattca accttttgct gtaccaggca atgccaaact cggcgataag atttgggaat
     4021 ctgacgatat taaaattccc gtctattgtg acaacaatac caacggcaat tttgaaagtg
     4081 agcatgttta tgcctgggta aacccctacc cgggggtgca ggatcgctat tatcaactcg
     4141 gcgtaactta caatggcgtt gattatgacg ccaaccaggg aaaaagccgc atcgacacta
     4201 accagtgtat tgacagtaag aacattgaca tttatacccc tgagcagatt atcgcgatgg
     4261 gatggcagaa caaaatttgc tcgggtgatc ccgctaatat tcacatgtca cgtacctttc
     4321 tcgctcgcat gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta
     4381 cgcttagcga ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagatccta
     4441 ctgcccaaaa cctgaaatat catattaccg gtctggaaaa cattcgcgtg ctggattgca
     4501 gcgtcaattt ttccatttca ccggaaacac aggtgattga ttttggtaaa tttaatttgc
     4561 tggatattcg tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa
     4621 atgaccaatg caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg
     4681 ttgaagaaga taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa
     4741 acgcctcgcc ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt
     4801 tggtctacga aaaaacctat acagctgaac tttcgtccat tgcaggtacc cccatcgagg
     4861 ctgggccctt cgatacagtg gtgcttttta aaattaacta taactgattt gccatgatgc
     4921 cctttgcagc cggaaagggc atctctttca gaaaattgct gattttcccc ttttccttga
     4981 ttgctgttat tttttcctgg cccggctttc tttatgctgt atcgatacac caaagagaaa
     5041 ataatgaggg agcgaaggat gccagttttg cagtggggaa tgttatgtgt gctatcactt
     5101 ctcctttcta ttggtttcct cgcggtacat ctcccggcgg cgctattact tgggccgatg
     5161 atcgccggga tcatcttcag tatgcgcgga attactctgc aacttccccg ctccgctttt
     5221 ctcgccgcgc aggccattct tggctgcatg attgcgcaaa acctcactgg ttcgattctc
     5281 accacgctgg ccgccaactg gccgatcgtg cttgctattt tactggtgac gctgctttcc
     5341 agcgccatcg tgggttggtt attggtgcgc tatagctcac tgcctggaaa taccggtgcc
     5401 tggggttctt ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga ttacggcgca
     5461 gatattcgcc tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc gggggcagct
     5521 gttctggtca cccgcatgat gctgggtgat aacgctgaag cggttaacca gcaaatcgtc
     5581 tggttcccgc cagtgagcat taatctcctg cttacgattt tgctcgcagt cgttgccggt
     5641 acggcgggat gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat gctggcgggc
     5701 gcagtgctcc agtctggtca gctcatcacc atcgaactcc cggaatggtt gctggcgatg
     5761 gcgtatatgg caattggttg gcggattggt cttggtttcg ataaacaaat cttactgcgg
     5821 gcattgcgcc cactgccaca aatcctgctg tcgatttttg ctctgctggc tatttgtgcg
     5881 ggtatggcgt gggggctgac ccggtttatg cacattgatt ttatgactgc ctacctcgcc
     5941 accagtcccg gcgggcttga taccgtggcg gtcatcgccg cagggagcaa tgccgatatg
     6001 gcgcttatta tggcgatgca aaccctgcgc ctgttcagta ttttgctgac ggggccagcc
     6061 attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga
     6121 caccagtaaa acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg
     6181 cacagttcgc catcacgatg aaaaacctta aagcgaaaca gcgccccatg atgcttattc
     6241 tcatccacct gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg
     6301 agtgcatcca gttgcgccgc attgagatct ttcgctttat gatttccagt caacccaacc
     6361 tgccagagga tctccacccg caaataattg ccaagcccag ccagaaacgc ctgatcgagc
     6421 agtaatcctg caaactgacg gttacgaaag cgcggtgaca ataaccgttc ctttaccacc
     6481 tccggcgtca gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg
     6541 gtcagttgtt ccggcctcaa catctcaata tccgaggcgc tataaagcag aatggttttg
     6601 tcagccgttt gcagttttac ccgcaatact cgcgtggtct gcggctcttc gccggtatca
     6661 accacgcgcc agacgccgta aagctgatta tggctgtaga gcgttaagtc gttggaaaaa
     6721 tgagttaaca acgccttacc acgcgtttcc acatgggtaa cgtgttgacc gataagttgt
     6781 gattgataag tttttaactg cgggaaggca aaccagacat cagttagtgg tttgcctttg
     6841 atcgccgcct ccaggttatc cgctgcacgg cggatctccg ggccttcagg catggtgata
     6901 tccttttttc attctttatt aatcggggtt atgctgcgac aacaatctcc ttttcggcaa
     6961 atgtagagcg tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca tgcaggcaga
     7021 ctgtttgcgc cgtgaccgtt gcccattcgc cggtgatact tttgactctg ccgtgttgca
     7081 ccatctccag cgtttgcgcc aacgcctgtt cttcgttttc aatcaacgcg cctcgctgac
     7141 ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg
     7201 ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa
     7261 tcaatgccgg atcgcaagcg tataccgctc tggcgatggc gtctgccagt tgtgcttctt
     7321 tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg acgcattacg ccgccttgcg
     7381 cacgggcaat cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa acggtttctg
     7441 gcggcagctg catggcgctg cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc
     7501 caatcgcgac accatttttt attgcttcac gcacgcaagc ctgcatgatt tgcgcatcgc
     7561 ctgcatgaaa tccacaggca atattggcag aggaaaccag cgttaatagc tctgcgtcgc
     7621 tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt cattgtgcag ccgccacgct
     7681 aattgttcga aataacgttg ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac
     7741 tggacaaaat gaatcggctg accgagcgga atttgcgcca gatggtacat atcagcctca
     7801 atgatacagg caatacgcgg gtaaccaccg gtggtctgtg cgtcgttcat caacacaatc
     7861 ggctgcccgt tatgcggcac ctgcaccacg cccggtaaca aaccgtgaga taacagttcg
     7921 cgatcggtga tgcgttttaa aatttgcccc tgtaagcgat agcccattcg gttactttgc
     7981 gagctaagct gccagggcga acgccagaat gcatcctgcg aggcgcgatc gaactcatga
     8041 tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc
     8101 gcttccataa aatcacgctt cgatttgcca atcgggagtc ggtcaccatc cttcagtaaa
     8161 cggccttcca gcccgccaat ccccactttg agatcggtgc tgcatgaccc cattaccggc
     8221 ggaacatcaa taccacccgc gaccgccaga taactgcgca tcccgtgctg cgggcgttta
     8281 agcgttaaac gctggcctgc tttcatcggc aatcgccagc cggtccagac ggcattatca
     8341 tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc acccatcagt ttcgaactca
     8401 accgttaact gaccgagcgt gatctccagc gcgggggcat tggcgtcatt acccaccagc
     8461 aggttagcaa tgcgtaacgc aggcatatcc agtgcgccgc agtggctgat acccgactgg
     8521 cgaaaaccgt gacgaccgcc atcttgcaca gtggtataca tgcccgcacg aataatcttc
     8581 agcatactcc ctccttttgc ggtacaaagc gcacgctgtc tcccggacgt aataagatgg
     8641 gttcgtcacg cgccggatca aacaggctga gtgaggtatg accaatcaac tgccacccac
     8701 ccggcgttgc cagcggataa acaccagtct gcggcccgcc gatcccgaca gaacctgccg
     8761 gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga
     8821 gatacgggaa gcccggttga aaacctaaaa accagaccac gtattccacg gaggagtgca
     8881 attcaacaac ctgtttttcg ctcaacccgc aatgcgccgc gaccaccgcc aaatccggtc
     8941 cgcctgcacc accgtaaacc accggaattt caataaagcg agactcaggt tccagggcct
     9001 cgctctcctc ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc gactcaggat
     9061 tacgcaaaat caccgtgata ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat
     9121 ccaccagacg ctgcgccagt cgccagatcc gtttctggct agccagcgtc accggcggtt
     9181 ccagttccag cactaccgcc gtttcaccta tcagataaca acgcgctcgt tgcactttcg
     9241 atccctctga ttattcgtta tgcaggatta ggtatatcaa taaaggtcac atcaagatcg
     9301 gtattttcat tcagccactc actcaatgcg cgaataccac cgcgttcagt ggcatggtga
     9361 cctgcagcat aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg ttcagaaact
     9421 tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg
     9481 ccgcccgtgc accaggcgac gcgctggacc acctcaggtc cggtatcgcc acaccataac
     9541 ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg cacaggcatg
     9601 gtcagctctc cccacggcac caacggctca atttcgccca tgaccgtgat ccccagtaac
     9661 gccgccagct gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa atgccagcca
     9721 tacaggttga tatcattcgc cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg
     9781 accggagact ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga
     9841 cgcactgcct catcgagcaa agcctggctg gcggtgacac cggtaacaat tttttgcacc
     9901 gtctctttgc cttccacctg caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc
     9961 agtttttcgt taatcagttg ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc
    10021 tgatagctac accgttaaca tagcgttacc tcgcctgttc gtcgatagcc aatcttttga
    10081 catattaaga aacaaacccg acgccaaaag acgcaaattg tttatatcat cacgcttcag
    10141 ctaatgtata tttatgcaat cagacagcat attgcataaa taatcagatc tgtatcaaag
    10201 ttttaacttt aaggaaacac aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt
    10261 gtggaattat tatgaataaa cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc
    10321 tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc cctgctgatt ctctatctca
    10381 ccaatcaact aaaatacaac gatactcacg cctacgagtt atttagcgcc tactgttcgc
    10441 tggtgtatgt cacgccaatc ctcggtggct ttttggcgga taaagttctc ggcaatcgca
    10501 tggcggtgat gctgggggcg ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg
    10561 agatccatcc gtcattcctc tatctgtccc tggcgattat cgtttgtggc tatggtctgt
    10621 ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg
    10681 atggcggttt ctcgctgatg tatgcagcgg gtaacgtggg gtctattatc gcacctatcg
    10741 cctgtggtta cgcccaggaa gagtacagct gggcgatggg ctttggcctg gcggcggtgg
    10801 gtatgatcgc gggtctggtc attttcttat gtggcaatcg tcatttcaca catacccgcg
    10861 gcgttaataa aaaagtactg cgtgcgacaa actttctcct gcctaactgg ggatggctgc
    10921 tggttctgct ggtggcaacg cctgcgctga ttaccgtatt gttctggaaa gagtggtcgg
    10981 tatacgcctt aattgtcgcg actattattg gcctgggtgt actggcaaaa atttatcgca
    11041 aagcagaaaa ccagaaacag cgtaaggagc tggggctgat tgtgacgctc accttcttca
    11101 gtatgttgtt ctgggccttc gcacaacagg gcggcagctc gattagcctt tatatcgacc
    11161 gcttcgttaa ccgcgatatg tttggttata ccgttccgac tgctatgttc cagtcgatta
    11221 atgccttcgc agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg
    11281 caggtaatcg taccgtgcgc atctggggaa aatttgctct tggtctcggc ctgatgagcg
    11341 ccgggttctg cattctgacc ttaagcgccc gctggtcctc aatgtatggt cactcttctc
    11401 tgccactgat ggtattaggc ctggcggtga tgggctttgc ggaactgttt atcgacccgg
    11461 ttgccatgtc gcaaattacg cgcattgaaa tccccggtgt gaccggcgta ttaaccggca
    11521 tttatatgct gctttccggc gcgattgcga actatctcgc gggcgtgatt gccgatcaga
    11581 catcgcaggc ttcgtttgat gcttccgggg cgatcaacta ctccatcaat gcatatattg
    11641 aagtatttga tcaaattacc tggggcgcac tggcgtgtgt aggactggta ctgatgattt
    11701 ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc gctggaatct taataggcca
    11761 gcaccatacc tgataaaacg ctcgctgcgt tttatcaggc tcctttcctc gcttcttcgt
    11821 acgctgccag cgttcgcagc cttgcctctt tgtgatcaac tatcggttgc ggataatcca
    11881 gcatcacacc tgctttctgc gcccacttcc acggctcatg caccgctttc cctggtacat
    11941 cgcgcagctc cggtaaccat cggcggataa actcgccctc acggtcaaat ttctcgccct
    12001 gagttatcgg attgaaaata cgaaaatacg gcgcagcatc agtacccgta gaagccgccc
    12061 actgccagcc accgttatta gctgccaaat caccatcaat cagctgcgac atgaaatatc
    12121 gctcgccttc gcgccagtcg atcaacaaat ctttcaccag aaaactggct gtaatcatcc
    12181 gtagccggtt atgcatccag ccagtactgt taagctgacg catagcggca tcaacaatcg
    12241 ggtatcccgt tttgcctttc tgccaggcct gtaaatgtgc gggattgctt tgccactgca
    12301 cacgatccgt ccaggcaata aacggacaat gtttacacaa cgaggggtaa tacgtcatca
    12361 gatgacggta gaactcgcgc cagatcagct cactaagcca gacactaccg gccccaccgt
    12421 ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc
    12481 ccgttgccag gctggcggac aaacgactgg tgccttccac tgccggaaaa tctcgttgtt
    12541 gctcatattc tccggcaccg ttctggcaaa actggcgtaa ttgcgcaatc gccgcttttt
    12601 cttccaccgg gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg
    12661 gcgcgggctc tatcgatccg ctactacgaa cttttggcgc agcgacacac tccggcatcc
    12721 cttcccgcag ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcgt
    12781 gattaccggt catcaccgcg ccaggcggca ggatcacgct gtcatcaaat ccttcacaca
    12841 ccacgttacg cagcgctctt tcaacttcca catcccgcgc ccgctcattc acttcatact
    12901 gatagttata aaacaggtgg gtaacgctgt tttccgcgca cacctgttta actatttcga
    12961 cactggcgac aaagtcatcc acttcacgga acaataaagg aatacctttt tccgcaagcg
    13021 ctatttgtag cccattcagt tgagcattga taagttcagc ctgacgcggc gacatgttat
    13081 gcgtcgccca ctggcgtggt gtagcgatat ataacgccag cacgcgtgca gacgaattgc
    13141 ggcaggcggc agccagtgcg agattatcgt gcagacgtaa atcctggcga aaccagacca
    13201 gatgggtaat cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa
    13261 ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat
    13321 cagcggcgca agaagtggtt gctcgccacg gcgataactg tcgataagcg ccgccaactg
    13381 ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt
    13441 ggtgtgatcg cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt ggtagtactg
    13501 gttgtaaaaa tcatccagat tttgccactg gtgaatatca gccacgaacg ggccaagttt
    13561 gcggtactcc ggctgagaat gcgccagaaa gacgagttta tagcggctgt gataatcgag
    13621 aagcgccccg cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat
    13681 acggattagg gtgctgtcat caaatcgttg ctgattcatt actgctcctg aacggtttac
    13741 aggagtaagt gtagacgttg agaaatgata tttgtgaagt taatcctgtt gtagcaggac
    13801 aaatttacga aagcctgctc cccgcaagga ctgacgtcag gtagtttcta accatagctg
    13861 ccctttctat tcaaacgtga aatcaataag ctcatcaggg acatcgcaaa caatatgaag
    13921 acgggtttct tctctgccgt gactcttttc actaattgcg taactgtcat gcttgggatt
    13981 atttaattct ttcagcggaa atttttcctc gaaggcttta tttagccgcc cctggtttcc
    14041 ttttacagcg aataaataat cacctccctg tttttgtatc ttctctgcaa tatctttctg
    14101 gcaacccatc gcatcggttg tgatgatttt tcctttaata tccagcatgt taagaagttc
    14161 agggatagct gtgatctcat tggatttctt atccgtcttg atctgcccga tgaccagact
    14221 gtgcattgtt gagaacgcac taatgacatg aatcgctccc ctgcggcgac tcttgtcata
    14281 agagtgccgg agcgtttttc catcaattgc aatgacgtct ttatcatttg atgaatggca
    14341 atcacgcatc cagttaataa agcactcgtg aaattttgca ggactgatac aggatacaac
    14401 tctggcaata gtatcgtgaa caggaatacc attttcaaaa tcaccatatt gcttcaagaa
    14461 atcgagatgt gtttcccc
//
LOCUS       DAZFWP020000123        14292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFWP020000123 DAZFWP020000000
VERSION     DAZFWP020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.123"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(129..209)
                     /gene="shoB"
                     /locus_tag="ACHK0Z_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             complement(129..209)
                     /gene="shoB"
                     /locus_tag="ACHK0Z_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU4503572.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(629..1009)
                     /gene="acpS"
                     /locus_tag="ACHK0Z_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             complement(629..1009)
                     /gene="acpS"
                     /locus_tag="ACHK0Z_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4503573.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(1009..1740)
                     /gene="pdxJ"
                     /locus_tag="ACHK0Z_RS18085"
                     /old_locus_tag="pgaptmp_003617"
     CDS             complement(1009..1740)
                     /gene="pdxJ"
                     /locus_tag="ACHK0Z_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU4503574.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1752..2480)
                     /gene="recO"
                     /locus_tag="ACHK0Z_RS18090"
                     /old_locus_tag="pgaptmp_003618"
     CDS             complement(1752..2480)
                     /gene="recO"
                     /locus_tag="ACHK0Z_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU4503575.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2492..3397)
                     /gene="era"
                     /locus_tag="ACHK0Z_RS18095"
                     /old_locus_tag="pgaptmp_003619"
     CDS             complement(2492..3397)
                     /gene="era"
                     /locus_tag="ACHK0Z_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU4503576.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3394..4074)
                     /gene="rnc"
                     /locus_tag="ACHK0Z_RS18100"
                     /old_locus_tag="pgaptmp_003620"
     CDS             complement(3394..4074)
                     /gene="rnc"
                     /locus_tag="ACHK0Z_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU4503577.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4347..5321)
                     /gene="lepB"
                     /locus_tag="ACHK0Z_RS18105"
                     /old_locus_tag="pgaptmp_003621"
     CDS             complement(4347..5321)
                     /gene="lepB"
                     /locus_tag="ACHK0Z_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU4503578.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5337..7136)
                     /gene="lepA"
                     /locus_tag="ACHK0Z_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             complement(5337..7136)
                     /gene="lepA"
                     /locus_tag="ACHK0Z_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU4503579.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7334..7813)
                     /gene="rseC"
                     /locus_tag="ACHK0Z_RS18115"
                     /old_locus_tag="pgaptmp_003623"
     CDS             complement(7334..7813)
                     /gene="rseC"
                     /locus_tag="ACHK0Z_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU4503580.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7810..8766)
                     /gene="rseB"
                     /locus_tag="ACHK0Z_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             complement(7810..8766)
                     /gene="rseB"
                     /locus_tag="ACHK0Z_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU4503581.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8766..9416)
                     /gene="rseA"
                     /locus_tag="ACHK0Z_RS18125"
                     /old_locus_tag="pgaptmp_003625"
     CDS             complement(8766..9416)
                     /gene="rseA"
                     /locus_tag="ACHK0Z_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU4503582.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9449..10024)
                     /gene="rpoE"
                     /locus_tag="ACHK0Z_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             complement(9449..10024)
                     /gene="rpoE"
                     /locus_tag="ACHK0Z_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU4503583.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10021..10176)
                     /gene="rseD"
                     /locus_tag="ACHK0Z_RS18135"
                     /old_locus_tag="pgaptmp_003627"
     CDS             complement(10021..10176)
                     /gene="rseD"
                     /locus_tag="ACHK0Z_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU4503584.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10432..12054
                     /gene="nadB"
                     /locus_tag="ACHK0Z_RS18140"
                     /old_locus_tag="pgaptmp_003628"
     CDS             10432..12054
                     /gene="nadB"
                     /locus_tag="ACHK0Z_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU4503585.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12039..12776)
                     /gene="trmN"
                     /locus_tag="ACHK0Z_RS18145"
                     /old_locus_tag="pgaptmp_003629"
     CDS             complement(12039..12776)
                     /gene="trmN"
                     /locus_tag="ACHK0Z_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU4503586.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12908..14242
                     /gene="srmB"
                     /locus_tag="ACHK0Z_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             12908..14242
                     /gene="srmB"
                     /locus_tag="ACHK0Z_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU4503587.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         3448 a         3822 c         3552 g         3470 t
ORIGIN      
        1 taattgatga cattagcata atcattcact aagttaattt atatagtatc tgccaagaca
       61 cttatttata gttattaaag gcgcgtccga ttggttcacc ggacgcacct taagtacgtt
      121 tccttgtgtt ataagaacag aaggatcagc tgtaaaacag caatgatgat tttgatgacc
      181 cgtttaatca ggtatcggca atcagtcatt cgtttttcct taaacaagga atgcagccgt
      241 tcagattagc ccttacatct ccccaaaact gaacgtgcga gttattgagg gtgcatgctg
      301 cactccacac cagagctttg acgacaccac tcgtttcaat gggggaattc tgtggcatgg
      361 tgtaaagcac agcaaaatct tcaataacga agccaatttt aatgtactta cgaattggcg
      421 gtcacgttaa tctttccatc aatattgctt tcttcgtaaa ggctcgagtt tttatgctaa
      481 agattgcaag ttgcttgtaa aagataagta cactgatcca taatcgctgt tgttgagggt
      541 gcatgctgca caaaattaaa gttaaaaagt aaaacccccg ttccttacca gttcgggggt
      601 tttacttttt aaagagaacg gtattatttt aactttcaat aattaccgtg gcacaagcat
      661 agtggcgttc atctgccagc gttacatgca tatttacaac gccgagcttt tccgccagtt
      721 ttaatgcctc accccatagc cgtagccgtg gtttgccgag ctcatcattg aatacttcaa
      781 attgattaaa cgccagacca ttgcggatcc cggtgccaaa cgcttttgct gcggcttctt
      841 tcacagcaaa acgcttcgcc agaaaacgca ccggctggtg gtgcgttttc cagattgccc
      901 attcgttatc gcttaatacg cggcgtgcca ggcgctcacc ggatcgggcg atcactgctt
      961 ctatgcgagc gatctctacg atatccgtgc ctaaacctaa tattgccatt agccacgcgc
     1021 ttccagcatc agacgcttca tttctgccac cgcatctttc agtccggtca tcactgcacg
     1081 accaataatg gcatgaccga tattcagttc atgcatctca gggatggcgg caatagcttt
     1141 cacgttgtga taggtcagac cgtgaccagc gttaactttc agaccgagac tagcggcaaa
     1201 ggtcgcggct ttggcgatac gcgccagctc ttgcgcctgt tcggcgtcag ttttggcatc
     1261 agcatagcaa ccggtgtgga tctcgataaa cggtgcgcca acctctgccg cagctttgat
     1321 ctgctcttca tcggcgtcga taaagagaga aacctggatc ccggcatctg ccagacgttt
     1381 gcaggcatcg cgcattttgt cacgctgccc tgcgacgtcc aggccgcctt cggttgttac
     1441 ttcctgacgc ttttccggta ccagacagca aaaatgtggc ttcgtctcaa cggcgatcgc
     1501 cagcatctct tcggtcaccg ccatctccag attcatgcgg gtatccagtg tctgacgcag
     1561 gatgcgcaca tcacggtcag taatgtggcg gcgatcttca cgtaaatgca cggtaatgcc
     1621 gtccgctccc gcctgctcag caataaacgc cgcctgcacc ggatccgggt aagcagtacc
     1681 gcgtgcgtta cgcagcgttg caatatggtc aatgttgacg cctaacagta attcagccat
     1741 gacaatcctc atcattcata atgtgttttc accgttcgct taggcataaa ctgccggaac
     1801 agttccctgc tctttaatgg tttaccgcca agatacggct taagcgccat gcgggtaaag
     1861 cgtttcgcgg cgcgtagtgt gtctgcgtca ggaaattccc gcgcgtttaa cgcttttaac
     1921 tgccttccgg tgaacgtttt attgtcgata acgacgcttg cgataaaccc tttttcttcg
     1981 cgataacgat acgtcatggt gtcatctacc ggctcgccgc tacccgcaca atgggtaaaa
     2041 ttgacgccat agcccagatg cccgagcagt gccagttcaa agcggcgcag cgcgggttct
     2101 ggcgtaccag tggcacctgc aagagactga atgcagtgca agtaatcgaa aaagagttca
     2161 gagaagcgcg tctcgtattc cagtacgcgg gagagaagtt cgttgatgta cagaccgctg
     2221 taaagcgtga taccgcttaa tggcagcgcc agcgagacag cttcagcact gcgcagcgtt
     2281 ttgacttcgc cacgcccgcc gaaacgtagc aagagagggg tgaaaggctg taatgcacct
     2341 ttcagagtag agcgtttaga gcgtgcgcct ttggcaacca gacgcacgcg acccgattcc
     2401 tccgtgaaga cgtccagcat caggctggtt tcgctccacg ggcgactatg caggacaaat
     2461 gcgcgctgcc agccttccat cggagtgact cttaaagatc gtcaacgtaa ccgagactgc
     2521 gcagtgcgcg ttcgtcgtcg gcccaaccgg atttcacttt tacccacagc tcaaggtgga
     2581 caggcgcttc gaacatttcc tgcatgtctt tacgcgcttc aatcccgatg gttttgatct
     2641 tggccccttt gttgccaata accatcttct tctgcccttc acgctcaacg agaatcaaac
     2701 cattgatgtc ataaccaccg cgttcgttag agacgaaacg ttcgatctcc acggtcacgg
     2761 agtatggcag ttcagcgccg aggaaacgca tcagtttttc gcggatgatt tcagacgcca
     2821 taaaacgctg tgagcgatcg gtgatgtaat cttccgggaa gtgatgggtc gcttcaggga
     2881 ggtgcttacg cacgattgct gcgatagtgt cgacattcag cccggtttca gcagagatcg
     2941 gcacgatatc gaggaagttc atctgacttg ccaggaactg taagtgcggc agcagatcgg
     3001 ctttttcctg cacgttatcc actttgttca ccgcgaggat caccggcgct ttgccatcgc
     3061 gcagtttgtt gagcaccatt tcgtcgtccg gcgtccagcg ggtgccttca acgacaaaaa
     3121 tcaccagctc aacgtcgcca atagagctgc tcgccgcttt gttcatcagg cggttaatag
     3181 cgcgtttttc ttccatatgc aggccagggg tatcaacgta gatcgcctga tacgcgcctt
     3241 cagtatggat ccccacaatg cggtgacgag ttgtttgcgc tttgcgggag gtgatggaga
     3301 tcttctgccc cagcagtttg ttcaacaatg tggatttgcc aacgttcgga cgtccgacga
     3361 tggcaataaa tccgcagtaa cttttatcga tgctcattcc agctccagtt ttttcaacgc
     3421 ctgttcggcg gcagcctgct cagccttacg acggcttgaa cctgtgccaa ccaccggttc
     3481 actcaggccg ctgacctggc agtggatagt aaattcctga tcgtgcgctt cgccacgtac
     3541 ctggactacc agataagtcg gcagcggcag atggcgaccc tgcaaatatt cttgcaagcg
     3601 cgttttcgga tctttttgtt tatcgcctgg gctaatttcg tccaaacgag tttgatacca
     3661 gttgaggatt aatttctcga cggtttgaat atcactgtcg aggaatacgc caccaattaa
     3721 tgcttcgacg gtgtcggcga gaattgactc acgacgaaat ccaccgcttt taagttcacc
     3781 tggccctaaa cgtaagcact cgcctaactc aaattcgcgc gccagttccg ccagcgtatt
     3841 gccacggacc agcgtggcgc gcatccggct catatcgcct tcatccacac gagggaaacg
     3901 gtgataaagc gcattggcga taacgtagct cagaatagag tcgcctaaaa attctaaacg
     3961 ctcgttatgt ttactgctgg cactacgatg agttaatgcc tgctgcaaca gttcctgatg
     4021 attaaaagtg tagcccagct tccgttgaag ccgattaatt acgatggggt tcatgcgata
     4081 ccaataaata aatgcgtcaa caattcagca cacgaaacag acctgatata catggctctg
     4141 ctaactgctt cgctgcagtt tcagtttacc ggtatatggg gaccaacgct gtttcgtgtg
     4201 ccgtggcaac ctggaggtgc caaccttaaa cttcggggga atattctata cacaacgacg
     4261 ggggatgtcg ttagccacgg gagatttatc tcataaataa ttcacgttgt cgccataacg
     4321 gcgacaacgt gaacgaagat gagctattaa tggatgccgc caatgcgact taagcgcaca
     4381 ccagtcggcc attcgccttc ttgcttatcg aagctcatcc agatagccgt tgcccgaccg
     4441 accagattcg cttcaggcac aaacccccaa taacggctgt ccgcgctgtt gtcgcggttg
     4501 tcgcccatca tgaagtattg tcccggagga acaatccagg ttgccagttg ttgccctggc
     4561 tgctggtaat acatccccac ctgatcctgc gcaatcggca ctgtcagaat tcggtgcgtc
     4621 acatcaccca gtgtctcttt acgctcggaa agacgaattc cattttcttt ggtttcgttt
     4681 ttcggcactt caaagaatcc gctggtcgct tccccaccat tacggcgtga gaaggtctga
     4741 acgaaatcgc tcggttccac gtttgagtag gtgaccggca gcgcgttttc acacgcctgg
     4801 ccggaactgc atcccggttg aatcgtcagc tctttcgaga ccggatcata agtaacttta
     4861 tcgcccggta aacccaccgc gcgcttgatg taatcaagct ttggatcttc cggatattta
     4921 aagaccacga tatctccgcg tttcggatga ccggtttcga tcagcgtttt ctggtagata
     4981 ggatctttaa tgccataagc aaacttttct accagaataa aatcaccgat taacagagtc
     5041 ggcatcatcg aacctgacgg gatctggaac ggttcataaa taaacgaacg cacaatcaat
     5101 acgatagcca gtaccggaaa aacagaagca ccggtttcca gccagccagg cttcggcgca
     5161 acctttttca acgttgcttt atccagtgag tcaccggcag ccgcctgcgc cgctgcctga
     5221 cgttcccgcc gtttaggtgc gaaaaagaat ttatccacgc accataaaat gcccgtcacc
     5281 agtgtggcaa tcaccagaat cagggcaaac atattcgcca tgccaactcc taagggttat
     5341 ttgttgtctt tgccgacgtg cagaatggcg aggaacgctt cctgcggcag ctcgacgtta
     5401 ccgatctgct tcatgcgttt cttaccttct ttctgcttct gcagcagctt tttcttacgg
     5461 ctgatatcgc cgccataaca tttagccagt acgtttttac gcagctgttt cacggtggat
     5521 cgcgcaataa tgtgcgtacc aatcgctgcc tgaatggcga tatcaaactg ctggcgtggg
     5581 atcagatctt tcatcttctc caccaactcg cgaccgcggt tttgcgaatt atcacggtgg
     5641 gtgatcagcg ccaacgcatc aacacgttca ccgttgatta atacgtctac acgtaccatg
     5701 tcggacgcct ggaagcgttt gaagttgtaa tccagagacg cataaccacg cgaggtagat
     5761 ttcaggcgat cgaagaagtc gagcaccact tccgccatcg ggatctcgta cgtcagcgcc
     5821 acctgattac cgtggtaaac catattggtc tgcacgccgc gtttttctac gcacagcgta
     5881 ataacgttgc cgagatatgc ctgcggcagc agcatgtgac attcggcaat cggctcgcgc
     5941 agttcgtaga tgttatttac cgctggcagc ttggatggac tgtcgacgta aataacttct
     6001 cttgacgtgg tttcaacttc atacactacg gtcggcgcag tggtgatcag atccagatcg
     6061 tattcacgtt ccagacgttc ctggatgatc tccatgtgca gcaggccgag gaagccgcag
     6121 cggaaaccaa agcccagcgc gctggagctt tccggctcat agaacagtga agcatcgttc
     6181 aggctgagtt tacccagcgc gtcacggaag gcttcatagt cgtcggaact taccgggaac
     6241 agaccggcgt atacctgcgg tttgactttc ttaaagccag gcagcgcctt ttctgccgga
     6301 ttacgcgcca gcgttaaggt atcgccgact ggagcgccgt ggatatcttt aatcgcacat
     6361 accagccagc ctacttcgcc acatttcagt tcagtgcggt caacctgttt cggcgtgaag
     6421 atgcccagac ggtcggcgtt ataggtctgc ccggtactca tgactttcac tttgtcgccc
     6481 ttacgcaggg tgccgttttt aatacggata agtgaaacaa cgcccaggta gttgtcgaac
     6541 catgagtcga taattagtgc ctgcaacggg ccttccggat cgccttccgg cggcggaatg
     6601 tcgcgcacca gacgttcgag aacgtcctgc acgccaacgc cggttttcgc tgaacagcgc
     6661 accgcgtcag tggcgtcgat gccgacgata tcttcaattt cttccgccac gcgttcagga
     6721 tcggctgccg gcaggtcaat cttgttcagt actggcacca cttcgagatc catttccatg
     6781 gcggtgtagc agtttgccag ggtttgcgct tccacgccct gcccggcgtc gaccaccagc
     6841 aatgcacctt cacaggcagc cagcgaacgg gaaacttcat aggagaagtc tacgtggccc
     6901 ggggtgtcga taaagttaag ctgataggtt tcgccgtcag acgctttgta gtccagcgtc
     6961 acgctttgcg ctttgatggt aatgccacgc tcacgctcaa gatccatgga atcgagaacc
     7021 tgcgcctcca tttcacggtc agacaggcca ccgcagatct ggataatacg gtcagacagc
     7081 gtcgatttac cgtggtcaat gtgagctatg atcgaaaagt tacgtatatt cttcataaag
     7141 tatgattatt gtgccttaat gcccggttaa ccaggctttt agaagtcgct gttctgagct
     7201 taacgtctgt attaatagaa acgccgcatt ctacactaca acattgaggc gaggaaatgt
     7261 tcataccgta tggattgtgg tatctggaaa cgtcctcgca tttgttatgc aaaatgcaac
     7321 aaagccagtg aaatcactgg ctcgcgtctt ccgaagatgt ttcaaatcgc acaaggccag
     7381 gcggcaatgc cacgcttaag atgatcggtt gccattctgc ccgggcagca aacttacgcg
     7441 agtagccgcg cgcaatcagg aacccgccaa taccaccgag gatcgcacca cataatgctg
     7501 cgacgtcaga agcaaagagt agctgaaata gcgaagcgat aaggaataat cccaccagcg
     7561 gcgacatata aaccagtaat gcggagctaa gcaggctgcc ttcggcgatc cccaattcca
     7621 ctttttgccc cggcactaac ggttcatcac agggtacgac aatggtatgc gtggtttgcg
     7681 ggccaagttt attcaacacg cggctaccgc aaccggcgcg tgaagcgcag ctgctgcatg
     7741 aagctttaac atcacaactg accagcgcct gcccgttttg ccaggagacg acggtagccc
     7801 actctttgat cattgcgctg ccccgaactt aatattctcg gcaatgcgtt tcgccgtttg
     7861 cggcggcagt tcaccgacaa tggtgatttc ggcgttatca cgtacgcttg tactgacggt
     7921 tctgcgtccg gtgcgcaaca tctgatcggt gctcgatggc gtagcgcggt taacgtttac
     7981 cgagaagctg aataatccgt cggaatagag acgtgattcg ataggcatgt tgtccatcgt
     8041 cggtagcgga cgtcgactac tggaaacttc gctaaaaccc tgtggcaacc aggttggcgt
     8101 ccagctgaat ttagcttttt cacctacagg aacagaaagc aacggcggca aatttgcctt
     8161 cgccagcgtc tgcatactgc tactgatatc ctgattgacg ttaaaagcaa tcacgcgaaa
     8221 ttgttccagc gtttcaccat cgcgatcaag gagatcaacc ctcatcggta atttcgattc
     8281 gatgtccatc cacacgatgt agctgtagcg tgtaccatct cgggcaacca cgcgaatgac
     8341 ttcgcaaaga cgatcagcaa tacgcgtgcg cccgacggag ataaagtcgt agtaaggaga
     8401 aaggcgtttg aaatcggtat agatgagcga tggcagagaa tcaacaatgt aatcgccatt
     8461 aagcgtgaac ggttcaagtc ccggttcaaa atagctgatt tcattgccgc gctgtaccac
     8521 ttcccggcgc gggccatcca tttgcaacaa ttgtgcaaga ggacggttat cgaggcgtgc
     8581 atgtcgataa cgcagagact caacaccctg tttattgatg ctgatgaatg acagctcgta
     8641 attcagtgac tgactggcca ggttcatctg ctgtaataac gccccggacg cgggagtggc
     8701 cgaggcgtta gcagagaata acaggctacc tgtcactaat gacatggcaa accaaagttg
     8761 cttcattact gcgattgcgt tcctaaagtt tgaattcctg gcacctgtac agcggcttgc
     8821 tgggtttgcg cctgctcaaa ctgaagctgt tcagagtgga gtcggcgttg cagttcgtaa
     8881 tcctgcaaca ttgcattaat gcgacgacgc tgctcctgta cctgctgctg ttgaccattg
     8941 tttgcggtcg cttcagaagg tactcccagg cttaccgggc tggctttacc catcatcggc
     9001 agtgtattaa ataccggcgt ttcgggctgc tgggacgttt cagattgtcc attatagtgc
     9061 tggacgccaa cgataactgc aagcgatacg catgctgcta cgcccatttg ggtaagctgt
     9121 gccgcccacg gacgtacttt ctgccagaat ggcattttct gccattgatg cggcgcaggc
     9181 tgggcttccg ggatcaatgt cgccggttga cgtactggct cttcttcaat ggcggccatc
     9241 acgcgtgaag agatatcgaa atggagcacc tcgggagtat caccccgcat tgagtcacgg
     9301 attaagtgat agctttccca ggttttctgc atttctgggt tatgagccag ttcgttaagc
     9361 agctcactat ccagcgtttc gccatccatt aaagcggaaa gttgttcttt ctgcatgcct
     9421 aataccctta tccagtatcc cgctatcgtc aacgcctgat aagcggttga actttgttat
     9481 caatagcttc cctcgctcgg aagatacgtg aacgcaccgt acctaccgga caatccatga
     9541 tagcggctat ctcttcatag ctcaggccat ccagctcccg caaggttatt gccatgcgca
     9601 aatcttccgg gagggactca atagttcgga aaactatctg tctcagttct tctgacaaca
     9661 ttaagttctc agggttcgaa atttctttca acgcgccgcc actttcgaag ttttcagctt
     9721 caatggcatc cacatcactg gaaggtggac gacgcccctg agcaaccagg taatttttcg
     9781 ctgtatttac agcaatccga tacagccatg tataaaaagc gctatctccc cggaacgaat
     9841 ccagcgcacg ataggcttta ataaaagctt cttgtaccac atcgggaaca tcacccgacg
     9901 gcacatagcg ggaaaccaga ctcgccactt tatgctgata gcgcactacc agtaggttaa
     9961 aggctttctg atctcccttc tggacccgtt caaccaggac ctggtccgtt aactgctcgc
    10021 tcatccgagg taaagtctcc ccaaaccaaa tttccacgcg ctatcgaaac gccactccat
    10081 tagctgcaat ttgagcaagc aaagggttag agtgtctcgt ttttgtaaag ttccgtaacg
    10141 catctgtttt tgtttgtcat gctgtagacg gatcattatc tatcattata agtctacaga
    10201 atctgaacat cgcattatct gtgtagaaat gcccatttaa ctgcctgaag agtaacccaa
    10261 cggccttttt atttcaccac ctaatcctcc accagccagt aacttctctt tttctcgccg
    10321 ccctgcgtca gcgtgtttag ccactgtaac aaatattaaa atagcaggtg tttattcgca
    10381 caacatgatg ctatgctgac caaacggtgt ttagtaaatt aaacaaagaa aatgaatact
    10441 ctcccagaac attcatgtga cgtgttgatt atcggtagcg gcgcagccgg actttcactg
    10501 gcgctacgcc tggctgacca gcatcaggtc atcgttctaa gtaaaggccc ggtaacggaa
    10561 ggttcaacat tttatgccca gggcggtatt gccgccgtgt ttgatgaaac tgacagcatt
    10621 gactcgcatg tggaagacac attgattgcc ggggctggta tttgcgatcg ccatgcagtt
    10681 gaatttgtcg ccagcaatgc acgatcctgt gtgcaatggt taattgacca aggggttttg
    10741 tttgataccc acgttcaacc gaatggcgaa gaaagctacc atctgacccg tgaaggtgga
    10801 catagtcacc gtcgtattct tcatgccgcc gacgccaccg gtagagaagt acaatccacg
    10861 ctggtgagca aggcgcagaa ccatccgaat attcgtgtgc tggagcgcag caacgcagtt
    10921 gatctgattg tttctgacaa aattggcctc ccgggcacgc gacgggttgt tggcgcgtgg
    10981 gtatggaacc gaaataaaga aacggtggaa acctgccacg caaaagcagt ggtactggca
    11041 accggcggtg cgtcgaaggt ttatcagtac accaccaatc cggatatttc ttctggcgat
    11101 ggcattgcta tggcgtggcg cgcaggctgt cgggttgcca atctcgaatt taatcagttc
    11161 caccctaccg cactgtatca cccacaggca cgcaatttcc tgttaacgga agcactgcgc
    11221 ggcgaaggcg cttatctcaa gcgcccggat ggcacgcgtt ttatgcccga ttttgatgag
    11281 cgcggcgaac tggccccgcg cgatattgtc gcccgcgcca ttgaccatga aatgaaacgc
    11341 ctcggcgcag attgtatgtt cctcgacatc agccataagc ccgccgattt tattcgccag
    11401 catttcccga tgatttatga aaaattgctc gggctgggga ttgatctcac acaagaaccg
    11461 gtaccgattg tgcctgctgc acattatacc tgcggtggtg taatggttga tgatcatggg
    11521 cgtacggacg tcgagggctt gtatgccatt ggcgaggtga gttataccgg cttacacggc
    11581 gctaaccgca tggcctcgaa ttcattgctg gagtgtctgg tctatggctg gtcggcggca
    11641 gaagatatca ccagacgtat gcctgatgcc cacggcgtca gtacgttacc gccgtgggat
    11701 gaaagccgcg ttgagaaccc tgacgaacgg gtagtaattc agcataactg gcacgagcta
    11761 cggctgttta tgtgggatta cgttggcatt gtgcgcacaa cgaagcgcct ggaacgcgcc
    11821 ctgcggcgga taaccatgct ccaacaagaa atagacgaat attacgccca tttccgcgtc
    11881 tcaaataatt tgctggagct gcgtaatctg gtacaggttg ccgagttgat tgttcgctgc
    11941 gcgatgatgc gcaaagaaag ccgggggctg cattttacgc tggattaccc ggaactgctc
    12001 acgcattccg gtccatcgat cctttccccc ggcaatcatt acataaacag ataaaaagcc
    12061 tgggtcagcg ccgtatacgc ttcggaatag ttctggtctg gcccacgaat gactaagcga
    12121 tcgctaaagc attctcccgc ctgcggggag aatgccagca gcacccgatg cggcagtcgc
    12181 gcttcgtttt ccgccacatc cgtccgcaaa cgtaaatgcc agcccatgct taatgccagt
    12241 tcagtaaaac cattaccaat ctgctctggc agcaccacgc agaaaaatcc ctcttcggta
    12301 atgcactccg ccgcacaggt cagcagcgat gggtgatcaa gcgtagtggt atagcgagcc
    12361 tgttcccgtt gaggtgtcgc gcactctact ccctgctgat agtaaggtgg gttactgatg
    12421 attaaatcga agcgtactgt ctgctgtgtg atccactgct gaatatccgc cgtatggacg
    12481 ttaatccgct ctgcccacgg ggactggttg atattttcct gcgcctgcgc ggcagcttca
    12541 ctttccagct caacggcatc aattatcacg ctgtcactgg ttcgctgcgc cagcatcagt
    12601 gccagcaacc cgctacccgc gccgatatca aggcaacgtt tcaccccagc caccggtgcc
    12661 catgcgccca gcaaaatgcc atccgttccc actttcatcg cacagcgatc gtgagcaaca
    12721 aaaaactgtt taaaagtaaa tccattacga cgaagcacgg atgtagactg tgacatgaaa
    12781 ataaaacctt gcaggaaaaa cggcgatagc accgggtgag aacaataccc gagaagcgat
    12841 atccatacaa acagatgaag attgcagccg taacgtctat aatcagcgcc ccacacagag
    12901 gtagaacatg actgtaacga ctttttccga acttgaactc gacgaaagcc tgctggaagc
    12961 cctccaggat aaaggtttca ctcgcccgac cgccattcag gctgccgcca ttccgcctgc
    13021 gctcgatggc cgtgatgtac tcggttctgc gccgacaggc accggtaaaa cggcggcgta
    13081 tctgctgcca gcgttgcagc acctgctcga tttcccgcgt aagaaatccg gtccgccgcg
    13141 tattttgatc ctcaccccaa ctcgcgagct ggcgatgcag gtggccgatc atgcccgcga
    13201 actggcgaaa catacgcatc tggatatcgc caccatcacc ggcggcgtag cctatatgaa
    13261 ccacgcggaa gtgttcagcg aaaatcagga catcgtggtc gccacgaccg gacgtctgct
    13321 gcaatacata aaagaagaga acttcgattg ccgcgcggtt gaaacgctga tcctcgacga
    13381 agcagaccgt atgctggata tgggcttcgc tcaggatatc gaacatattg ctggcgaaac
    13441 gcgctggcgt aaacagaccc tgctcttttc ggcaacgctg gaaggcgatg cgattcagga
    13501 ctttgccgag cgtctgctgg aagatccggt ggaggtttct gccaatccgt ccacccgtga
    13561 gcgcaaaaaa attcatcagt ggtattaccg cgccgatgac ctggaacata aaaccgcatt
    13621 gctggtgcat ctgttaaaac agccggaagc gacccgctca attgtgtttg tgcgtaagcg
    13681 tgaacgtgtg catgagctgg caaactggct gcgcgaagcg ggcatcaaca actgctatct
    13741 cgaaggtgag atggtacaag gcaagcgtaa cgaagcgatc aagcgtttga ccgaaggtcg
    13801 cgtaaacgta ctggttgcaa ccgatgttgc cgcgcgcggt atcgacattc ctgacgtcag
    13861 ccacgtcttt aacttcgata tgccgcgcag tggcgatact tatttgcacc gtatcggacg
    13921 taccgcgcgc gccggtcgta aaggcaccgc aatttcgctg gtggaagccc atgaccattt
    13981 gctgctgggc aaggttggcc gttacattga agagccgatt aaagctcgcg ttattgatga
    14041 gttacgcccg aaaacgcgtg cgccaagcga gaagcagacc ggcaagccat cgaagaaagt
    14101 actggcaaaa cgcgccgaga agaaaaaagc taaagagaaa gagaaaccgc gtgtgaaaaa
    14161 acgccatcgc gacaccaaaa atattggtaa gcgccgtaaa ccaagcggaa cgggcgtgcc
    14221 accgcaaacg acagaagagt aatctcaatg ccgggtttaa gcctggtatt aaaagtgctg
    14281 gcaaacgcaa aa
//
LOCUS       DAZFWP020000124        14196 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFWP020000124 DAZFWP020000000
VERSION     DAZFWP020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.124"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            163..1221
                     /gene="citC"
                     /locus_tag="ACHK0Z_RS18155"
                     /old_locus_tag="pgaptmp_003631"
     CDS             163..1221
                     /gene="citC"
                     /locus_tag="ACHK0Z_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU4503588.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1236..1532
                     /gene="citD"
                     /locus_tag="ACHK0Z_RS18160"
                     /old_locus_tag="pgaptmp_003632"
     CDS             1236..1532
                     /gene="citD"
                     /locus_tag="ACHK0Z_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU4503589.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1529..2437
                     /gene="citE"
                     /locus_tag="ACHK0Z_RS18165"
                     /old_locus_tag="pgaptmp_003633"
     CDS             1529..2437
                     /gene="citE"
                     /locus_tag="ACHK0Z_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU4503590.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2448..3980
                     /gene="citF"
                     /locus_tag="ACHK0Z_RS18170"
                     /old_locus_tag="pgaptmp_003634"
     CDS             2448..3980
                     /gene="citF"
                     /locus_tag="ACHK0Z_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU4503591.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3984..4535
                     /gene="citX"
                     /locus_tag="ACHK0Z_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             3984..4535
                     /gene="citX"
                     /locus_tag="ACHK0Z_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU4503592.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4510..5388
                     /gene="citG"
                     /locus_tag="ACHK0Z_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             4510..5388
                     /gene="citG"
                     /locus_tag="ACHK0Z_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU4503593.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5439..6902
                     /gene="citT"
                     /locus_tag="ACHK0Z_RS18185"
                     /old_locus_tag="pgaptmp_003637"
     CDS             5439..6902
                     /gene="citT"
                     /locus_tag="ACHK0Z_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU4503594.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7016..7822
                     /gene="rna"
                     /locus_tag="ACHK0Z_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             7016..7822
                     /gene="rna"
                     /locus_tag="ACHK0Z_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU4503595.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8052..8462
                     /gene="rnk"
                     /locus_tag="ACHK0Z_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             8052..8462
                     /gene="rnk"
                     /locus_tag="ACHK0Z_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU4503596.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLRIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8600..8689
                     /gene="yldA"
                     /locus_tag="ACHK0Z_RS18200"
                     /old_locus_tag="pgaptmp_003640"
     CDS             8600..8689
                     /gene="yldA"
                     /locus_tag="ACHK0Z_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU4503597.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8693..9931)
                     /gene="ybdR"
                     /locus_tag="ACHK0Z_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /pseudo
     CDS             complement(8693..9931)
                     /gene="ybdR"
                     /locus_tag="ACHK0Z_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10152..10580
                     /gene="uspG"
                     /locus_tag="ACHK0Z_RS18210"
                     /old_locus_tag="pgaptmp_003642"
     CDS             10152..10580
                     /gene="uspG"
                     /locus_tag="ACHK0Z_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU4503598.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10701..12266)
                     /gene="ahpF"
                     /locus_tag="ACHK0Z_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             complement(10701..12266)
                     /gene="ahpF"
                     /locus_tag="ACHK0Z_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU4503599.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12395..12958)
                     /gene="ahpC"
                     /locus_tag="ACHK0Z_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             complement(12395..12958)
                     /gene="ahpC"
                     /locus_tag="ACHK0Z_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU4503600.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13330..14076
                     /gene="dsbG"
                     /locus_tag="ACHK0Z_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             13330..14076
                     /gene="dsbG"
                     /locus_tag="ACHK0Z_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU4503601.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3364 a         3752 c         3574 g         3506 t
ORIGIN      
        1 tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcac
       61 ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa caacattcgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa
      361 tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat
      421 gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc
      481 cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat ggaaaacagc
      541 gccacgcggc tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaag
      601 attggctgca ttgtgatgaa cgccaatccc ttcacgaatg gtcaccgtta tctgattcaa
      661 caagctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      721 ttcccctatg aagaccggtt ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      781 actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg ctacttcatt
      841 aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat tttccgtcag
      901 tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
      961 gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac tatctccgca
     1021 ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1081 tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1141 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1201 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1261 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1321 tcgacctgca aatcaatagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1381 ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag
     1441 gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc gccagcggta
     1501 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1561 tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg tcagcaactc
     1621 cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtgg cattacgtga
     1681 aaaagacacc gcccgccgca tggtttatca cgcgctgcaa catccgctgt atcgcgatat
     1741 tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgttaacg acctggaagc
     1801 cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1861 tctggatatt gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag
     1921 caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc
     1981 tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct
     2041 gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt ccattttgca
     2101 ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2161 agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg gcaaatcgct
     2221 gatcaacccg cgtcagattg atctgttgca caacctctac gcaccaaccc agaaagaagt
     2281 ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2341 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2401 ctctcgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa
     2461 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc
     2521 ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2581 aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac ggcatgacgg
     2641 tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2701 tcgcgaagat gggctttaaa aacctgaccc tggcgtccag ctccctgagt gattgccatg
     2761 cgccgttggt agaacacatt cgccagggcg tggttacccg catttatacc tccggcctgc
     2821 gcggtccact ggcggaagaa atctcccgcg gtctgctggc ggaaccggtg cagatccact
     2881 ctcacggcgg tcgtgtgcat ctggtacaga gcggcgaact gaatatcgac gtggctttcc
     2941 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt aaagcctgct
     3001 gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtt gtgatgctta
     3061 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatt
     3121 taatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt gctacccgta
     3181 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgtcaact
     3241 caggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgca tcgctggcgg
     3301 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3361 gtggtattac cgcgacgatg gttgatctgc acgaaaaagg tctgatccgc aaactgctgg
     3421 atgtacagag ctttgacagc cacgctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3481 aaatcagcgc caaccagtac gctaactggg gttcgaaagg tgcatcggtt gatcgtctcg
     3541 acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg
     3601 gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg attgcctctg
     3661 cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgactctg gtggataacg
     3721 tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac cacggtatcg
     3781 cagttaaccc ggcacgtccg gagctggcag aacgtctgca ggaagcgggc attaaagtgg
     3841 tttccattga gtggctgcgt gaacgtgcgc gtctgctgac cggtgaacca cagccgattg
     3901 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     3961 tacatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4021 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgtctggc
     4081 tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag
     4141 acagtgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt gccttagccg
     4201 caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4261 gtatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4321 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgaaattc
     4381 tctcccgccg cgactattca ctgccgcctc gcagctgcct gttgtgcgag caaagcgcag
     4441 ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcac
     4501 tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta
     4561 atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg
     4621 aaaccaggcc tcgtggatcg gattaactgc ggtgcgcata aagatatggc gctggaagat
     4681 ttccaccgca gcgcgctggc gattcagggc tggctacccc gtttcattga atttggtgcc
     4741 tgtagtgcgg aaatggcacc agaagcggta ctccacggat tacgcccaat tggtatggct
     4801 tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg cagcattttt
     4861 tctttagggc tgctatgtgc ggcaattggc cgtttgcttc agctcaacca gccggtaacg
     4921 ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gtctgaccga tcgcgaactg
     4981 cgtaccaata attcacaact gacggcaggt caacggttgt accagcagct tggccttacc
     5041 ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc cttgccgcat
     5101 tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga taccttgctc
     5161 ctactgatgg cgatcaacgg cgataccaac gttgcttcgc gcggtggcga ggggggcctg
     5221 cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg aacccccgcc
     5281 gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc
     5341 ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt atttaagcac
     5401 ttgataaatt tgaaaatatt aattttcgga gaacccgtat gtctttagca aaagataata
     5461 tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc cctgtccccg
     5521 acggtatgcc accacaggca tggcattact tcgctgtgtt tgtggcaatg attgtcggca
     5581 tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact atttgcgtta
     5641 ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg tttaatgcgc
     5701 aaaaacaggc gctgaaatgg ggtctggctg gtttttccag caccacggta tggctggtat
     5761 ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt cgcattgccc
     5821 ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg attgtcatta
     5881 tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt
     5941 ttccggtcat taaaaacctg ccgccgttgt ttaaatcatt ccccaacgat ccgtccgcgc
     6001 gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg agttcgtcca
     6061 tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc aaaattgccg
     6121 gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg gttatcttgc
     6181 ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca cacagtgaag
     6241 aagtggcaac ctgggcgggt gatgaactga aaaccatggg tgcgctgaca cgcagagagt
     6301 ggacactgat tggccttgta ttgctcagct taggtttgtg ggtttttggc agtgaagtca
     6361 ttaatgctac tgcagttggt ctgctggcag tttcgctaat gctggccctg cacgttgtgc
     6421 cttggaaaga tattacccgc tataacagcg catggaacac gctggtcaac ctggcaactc
     6481 tggttgtgat ggctaacggc ctgactcgtt ctggctttat tgactggttc gccggtacca
     6541 tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg gttctggtgt
     6601 tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc atgctgccgg
     6661 ttattctggc cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg tgtatcctgc
     6721 tggtgctgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt cctggggtga
     6781 ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc gcaatcttcg
     6841 gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc atgtggaact
     6901 aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggtaagccc acctggcggg
     6961 cttttttatg atttaataga tagtattcag tgtatgtatg agttccacac ccattatgaa
     7021 agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttct cttctgccaa
     7081 cgccttagcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc tggccctctc
     7141 ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac gagatgaatg
     7201 tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg gtctgtggcc
     7261 aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga tgcgcttcgg
     7321 ttgcgctact cgcccaatcc cgaatctacc agaagcgcac gccagccgga tgtgttcatc
     7381 gccggaaacc ggattatcac tggaaacggc cgctaaacta agtgaagtca tgccaggagc
     7441 tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct gctttggttt
     7501 tgatccggac gcatacttcg gtacgatggt acgcctgaat caagaaatta aagagagcga
     7561 agccggaaaa ttccttgcgg ataattacgg taaaacagtg agccgccgtg actttgacgc
     7621 cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa cgtgccaggg
     7681 taaccctgcg tatttgactg aaattcagat ctcgatcaaa gctgacgcca tcaacgctcc
     7741 gctttctgca aactcattct tgccacaacc acacccaggc aactgtggca aaacctttgt
     7801 gattgataaa gtgggttatt aatcatctga tttacaaaac acgctaattc gtcttccata
     7861 aaccgtgacg attaagaatt atacataatg cgctgcattg aggaattccc tgaatgtgac
     7921 gcaaatcact tcaaagagtg agcaacagtc agtatcatca tgtgagttaa accctcgccg
     7981 cctgacggtg agggttttct tttgggtatg tttatctgcg acactcgcag taccgacgac
     8041 atggagtaaa aatgtccaga ccaactatca tcattaacga cctggatgcc gaacgcatcg
     8101 atattctgct ggagcaaccc gcctatgctg gtttgcgaat cgccgacgcg ttaaacgcag
     8161 agttggatcg cgcccaaatg tgttcgccag aagagatgcc acacgacgtg gtgacaatga
     8221 acagccgggt taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt
     8281 atccggcaaa aatgaccgat agcaatactc agctttccgt tatggctccg gtaggtgccg
     8341 cactgctggg gctgcgcgtt ggcgattcca ttcactggga acttccgggc ggcgttgcaa
     8401 cccaccttga agtgctggaa ctcgaatacc agccagaagc tgctggcgac tacctgcttt
     8461 aatcccatct gcacaagcga taactcgctt tgcccagagg atgcactcgc atcctcttta
     8521 cgcaaaatca gcaatttttc ctggttctca ctgttcactt ttgctgcaaa attattccgt
     8581 ttcgtttgtg ggaataacca tggcagaagc attttacatc ctgataggat ttctgattat
     8641 ggcggcgatt atcgtcatgg ctgtccttta cctcgaaaac cactcctgac gatcatattg
     8701 ttccccccgg catcgcattc accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt
     8761 gtgcaccggg taccagaatc accttacggc actcctcttc acgtttttcg aaaatctcat
     8821 atccccgggc ggcctcttaa aacggcatat agtgggtaac aatttcttct ggtttcagta
     8881 atcctttctc aattaacggt aataattctc ccagccatgc gtgaacgtgg gtctgtccca
     8941 ttttaaacgt caacccttta tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag
     9001 cgtagacgcc cggtacgcta acaatgccgc cacgcctgac cgccgcaata cactggcgca
     9061 acgctttacc gctgctgccc tccagtttca ggttagtcag caccgtttcc gtggtgctgc
     9121 ctttcgcttc aaaaccgacg gcgtctatta ctgcatccac gccccggtga cctgccgttt
     9181 gttcaataat tgactgtgcc ggatcgctat cttcatcgaa attaatcggg atcgcgccgt
     9241 agcggtcagc ggcgaaacgc aagcggtagg gatgatgatc aacaacaaaa atctgttccg
     9301 caccgagcaa ccgtgcacag gcgattgtca acaatcccac tggaccggca ccatagactg
     9361 caacgcttga accttgttgg atctgcgcat tttttgctgc ctgccatgcc gttggcagaa
     9421 tatcagaaag gaaaagggct ttatcatctg aaagcaaagg cggtacttta aacggcccca
     9481 cattcccttt agggacgcgg acatattccg cctgtccacc aggaacgcca ccatacaggt
     9541 gactataacc aaacaatgcc gcgggagctg gtatctgttt tttattgagc gcagcgcctt
     9601 tacccgcatt ggtattttcg caggcggcat attgctgcat tcgacagaaa aaacagtcgc
     9661 cacaagcaat gacaaacgga attaccaccc ggtcgccttt ttgcaaattt tttacgtcct
     9721 ttccggtttc aacgacttcc cccataaatt catgaccaaa aatatcgcca tgtttaacct
     9781 ggggtatttt gcctcgataa agatggaggt cagagccaca gatcgccgtt gccgtaatac
     9841 gcagaataat atcatctgcc tgttcaatgc ccggatcggg aacattttct acctgaacgt
     9901 gatgtgggcc gtgataagtc aatgctttca tgcaacctcc agagacagtt caaaaaagag
     9961 tcagtcttta agggtagcta tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga
    10021 attcttggtg ttcagtgaga atatattaat cacagaaacc gttcgtaagg aaaaagaacc
    10081 agacaaatat cagggataaa tattataatc atggcgaatg atttgttcat gattaacagg
    10141 gagaaagggt tatgtataag acaatcatta tgccagttga tgtatttgaa atggaattga
    10201 gcgacaaagc tgttcgccac gctgaattcc tcgcccagga tgacggagtt attcatctac
    10261 ttcacgtact acccgggtca gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc
    10321 gttttgaaga gcatctgcaa catgaagcag aagaacgtct gcaaacgatg gtcagccact
    10381 tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg cgggatgaag
    10441 tcaatgagtt ggcagaagaa ctgggggctg atgttgtagt tattggttct cgcaacccat
    10501 cgatttcgac ccatctgtta ggttctaacg cctcgagcgt gatccgccat gccaatctgc
    10561 cggtgctggt tgtgcgttaa ttgccagaat aaatatcccg ccctgcccgg agccatcccg
    10621 gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc taagcaattg
    10681 caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc
    10741 agagaggctt tggcaccttc gccagtggcg atgatgatct gcttgtacgg aaccgtcgta
    10801 cagtcacccg ctgcgaatac gcctttgacg ttggtttcgc atttcgcatc aatgataatc
    10861 tcacccatgc ggttacgttc gactgcgcct tccagccagt tggtgttcgg cagcagacca
    10921 atctggacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac acgatctcga
    10981 tattccagac caacgacttt gctgccgtcg cctttcactt ccgtggtttg cgcattcaga
    11041 ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gcacctggtc ggctttcatt
    11101 tctggcgcaa attccagcag agttacgtgc tcaacgatac ccgccaggtc gattgccgct
    11161 tccacgccgg agttaccgcc gccgataacc gctacgcgtt tgcctttaaa cagcgggccg
    11221 tcgcagtgcg ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc cggaacgttc
    11281 atgttgcgcc attttgcacc ggtcgccacg ataatgctgc gtgctttcag taccgcgcca
    11341 gaagctgttt caatctggtg tagaccacct tcaaccgctg ccgggatcag tttgctggcg
    11401 ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc
    11461 agcttctgcc cttcagtctt cggtacagaa atgtagtttt cgatatcaac ggtatcgagg
    11521 atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg tgcggagtaa
    11581 attgctgccg ctgcacccgc cgggccggaa ccgacgatta atacgtcata agcatcacgc
    11641 ttgttcagct cttctgccgc acgtttttcc gcgccagtat caattttggc aacgatttca
    11701 gtcaacgtca tgcggccctg accaaactct ttcccgttta cgaacactgc cggaacgccc
    11761 atcacgttgc gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg
    11821 atgcgcgggt tcagtacgct catcaggttc agcgcctgca ccacgtccgg gcagttgtgg
    11881 caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg aatctgctcc
    11941 agcagagact gcgcttcttt cgacggatga ccaccggtcc acagcaacgc cagtaccagc
    12001 gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gcccctggtt ggaacctggg
    12061 ttggtgatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt aaaggtgact
    12121 ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga tttagcgctg
    12181 tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta agccttgagt
    12241 tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat catcatgcaa
    12301 gcggcctctg cggctacctg gatgcagctt gcatcatggt gcggggaaga aaatcgggtg
    12361 ccgcagcacc cgattaatta cgcgattgcg gaacttagat tttaccaacc aggtccagag
    12421 acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag
    12481 aagctacgta ctgtgctgct ttgattttac gcagcaggtc agacgcgtca cggccaatgc
    12541 cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac
    12601 ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg
    12661 ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt
    12721 tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt
    12781 cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg
    12841 ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt
    12901 cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct
    12961 atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat
    13021 gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga
    13081 actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa
    13141 tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa
    13201 atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag
    13261 ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag
    13321 gacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca
    13381 gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaacg
    13441 ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc
    13501 atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt
    13561 gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg
    13621 tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt
    13681 gtctacgtgt tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc
    13741 ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca
    13801 gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa
    13861 gaatatgaat cctctggtgg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagaa
    13921 caaatgaaag tgttaagcgc caacgagaaa ctcatggacg atctgggggc aaatgtcaca
    13981 ccagcgatct attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat
    14041 cagaaaacgc ttaatatcat tatggggaat aaataaaaga taattcatgg atggttgaaa
    14101 tacatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctctg ccctaagtgg
    14161 ctatgctatt ttagaaaaac ttaatcctga gttaag
//
LOCUS       DAZFWP020000125        14050 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFWP020000125 DAZFWP020000000
VERSION     DAZFWP020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.125"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            10..1301
                     /gene="yjcF"
                     /locus_tag="ACHK0Z_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /pseudo
     CDS             10..1301
                     /gene="yjcF"
                     /locus_tag="ACHK0Z_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(1454..3103)
                     /gene="yjcE"
                     /locus_tag="ACHK0Z_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             complement(1454..3103)
                     /gene="yjcE"
                     /locus_tag="ACHK0Z_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4503602.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3254..4603)
                     /gene="ghxP"
                     /locus_tag="ACHK0Z_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /gene_synonym="yjcD"
     CDS             complement(3254..4603)
                     /gene="ghxP"
                     /locus_tag="ACHK0Z_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU4503603.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(5149..5613)
                     /gene="soxR"
                     /locus_tag="ACHK0Z_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             complement(5149..5613)
                     /gene="soxR"
                     /locus_tag="ACHK0Z_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU4503604.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5699..6022
                     /gene="soxS"
                     /locus_tag="ACHK0Z_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             5699..6022
                     /gene="soxS"
                     /locus_tag="ACHK0Z_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU4503605.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(6025..7611)
                     /gene="pdeC"
                     /locus_tag="ACHK0Z_RS18255"
                     /old_locus_tag="pgaptmp_003651"
     CDS             complement(6025..7611)
                     /gene="pdeC"
                     /locus_tag="ACHK0Z_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU4503606.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            8041..8322
                     /gene="yjcB"
                     /locus_tag="ACHK0Z_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             8041..8322
                     /gene="yjcB"
                     /locus_tag="ACHK0Z_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU4503607.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8421..8957)
                     /gene="ssb1"
                     /locus_tag="ACHK0Z_RS18265"
                     /old_locus_tag="pgaptmp_003653"
     CDS             complement(8421..8957)
                     /gene="ssb1"
                     /locus_tag="ACHK0Z_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU4503608.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            9211..12033
                     /gene="uvrA"
                     /locus_tag="ACHK0Z_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             9211..12033
                     /gene="uvrA"
                     /locus_tag="ACHK0Z_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU4503609.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(12068..12424)
                     /gene="yjbR"
                     /locus_tag="ACHK0Z_RS18275"
                     /old_locus_tag="pgaptmp_003655"
     CDS             complement(12068..12424)
                     /gene="yjbR"
                     /locus_tag="ACHK0Z_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4503610.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12428..12844)
                     /gene="yjbQ"
                     /locus_tag="ACHK0Z_RS18280"
                     /old_locus_tag="pgaptmp_003656"
     CDS             complement(12428..12844)
                     /gene="yjbQ"
                     /locus_tag="ACHK0Z_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU4503611.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12955..13668)
                     /gene="aphA"
                     /locus_tag="ACHK0Z_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             complement(12955..13668)
                     /gene="aphA"
                     /locus_tag="ACHK0Z_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU4503612.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3750 a         3583 c         3348 g         3369 t
ORIGIN      
        1 ggatatttta tgcgctataa tggtttaaat aatatgtttt ttcctctttg ccagattaat
       61 gataaccact ctgtcacaag ttcatcacat acaaagaaaa caaaatcaga taattacagc
      121 aaacatcata aaaacacgtt aattgacaat aaagccctct ctcttttcaa aatggatgat
      181 catgaaaaag tgataggctt gattcagaaa atgaaaagaa tttatgatag tttaccatca
      241 ggaaaaatca cgaaagaaac ggacaggaaa atacataaat attttataga tatagcttca
      301 tatgcaaata ataaatgtga cgatagaatt acgagaagag tttaccttaa taaagataag
      361 gaggtgtcaa ttaaggtggt atattttata aataatgtca ccgtccataa taatactatc
      421 gatatcccac aggcagaaaa tggtggttac gatttttcac accttagcct gaaaggtatc
      481 gtgattaaag atgaagattt atccaattcg aattttgcag gttgcagact acaaaactct
      541 attttccagg actgtaatat gtatagaacg aattttattg cgccataatg gaaaaaatac
      601 tttttgataa ttgtattctc gatgactcat atttcgctca tgtaaaaatg actgacggga
      661 ctctaaatgc atgctccgct atgcattttc aattctacaa tgcagcaatg aatagagcca
      721 atattaaaaa taccttcctt gactattcaa atttttatat ggcgtacatg gctgaggtaa
      781 atctttataa agtaatagcg ccatatgtta atttatttaa agccgacctt agtttctcta
      841 aactcgattt aattaacttt gaacatgctg atctgtctcg cgtcaatctg aacaaagcaa
      901 tcctccagaa tataaactta attgatagca aactcttttg tacgtggctg acaaatacat
      961 tcctcgaaat ggttatatgt accggctcta atatggctaa ggttgatttt aatgatgcca
     1021 atttaagcaa ctgccatttc aactgttcta ttttaacaaa agcctgtatg tttaataccc
     1081 gtctctatcg ggttaatttc gatgaggcta gcgtccaagg aatgggcatt tccattctcc
     1141 gtggggagga aaatatcccc attgatagtg ataccctggt aacactacag aaattctttg
     1201 aagaagattg tacctctcat actggcatgt cacaaactga ggataatatt aatgcagccg
     1261 ctatgaagat tactgcagat attatgcaac acgcagattg aggttaagtc gaagaacagc
     1321 caatgaattg actttacaag aggataactt atttgtttta gaatcatgaa tgttagcccg
     1381 agctaacctg ttaaaggtgc aaaagattgc acctttttca gattacctcg ggcctggtta
     1441 cgtcgctttc ggcttactga ttttcctcaa ttagcaacgc ttcaagcaaa tcgagatcgt
     1501 gcagtaattt ttgcagcgtt tcgttgctga tctcccgcgt ggcgcgcagg tggtacagtt
     1561 cagcacgttc agaacgcaat gccgccagac ggaagcgacg ctcaaggttc tcttcctgca
     1621 cggaactttc aacgtcgtta cgtccatcgg cgcgacgacg caggttacca atgacgcgag
     1681 aactgacctc cgtaagcagc tggttatcga tattctcttc ggtatccgcc gccagacgct
     1741 cctccatttt ctggatcgcc acaatcgcca cttctgccgt tgccgctcgc gcaatacgtt
     1801 cttctttcaa ttgttgcgaa tggtccgcga cttcaatgtg ttgtagcaga ataggcaaca
     1861 tcaccacgcc gacaaacagc gagaagagaa tgacgccagc cgccaggaac accagctcat
     1921 agcgcgccgg gaagacgtta ccatccggca agagcagcgg gattgagagc acaccggcca
     1981 gagtgatcgc cccacgcacc ccggcgaaag acgcgatcag gatttctcgt gtggtccacg
     2041 aaccaaactc catcggcttc ttcttcagga agcggttgct gaactttttc atcgtccaca
     2101 gccagccgaa acggaccagc atcagcgccg catatatcag aataatatcg gtaaacagca
     2161 tccagatttc gacgttaggg tcgatttctg ctgccatcag cgacgtctcc agaatacccg
     2221 gcagctgcag acctaacagc aggaacacca tgccgttaaa gacaaattcc agcatcgccc
     2281 aggtgctgtt tgcacgcagg cgcattgcca gcggcgcacg gcgcatcaca ccggagcggg
     2341 tgatggtcat cccggcggca accgcagcga ggatgccgga aacgccaata tgttcggcaa
     2401 tcagataaga agcgaatggc agcaagaaca gcagaacaat ctgcgtcgcg ggttcatcac
     2461 cgccccagcg gctgaggaat cgcagcgagc gaccatacag ccagctcacc acaaaaccgg
     2521 caagaatacc gccaatggct actttcataa attcgaccgt cgcaccgccg acggtgaaga
     2581 tcatcgtccc catcgccact gccacggcaa acttcaacga caccaggccg gaagcgtcat
     2641 tcatcaacgc ttcgccctgc aaaatgccca tgattttttt cgggatgcgc ccttcgccaa
     2701 caatcccgga gagcgccaca gcatcggtcg gagaaagtac cgccgccagc gcaaaggcgg
     2761 ggatcagcgg aatgcccggc accacccagt aaataaggaa gccaatgccg accacggtga
     2821 ccaccaccag cgccagcgcg aggccgaaaa tctctcgacc atgttcgaga aattcacggg
     2881 tcggcgtttt ccagccatca gcgaacagca acggcgggat aaacaagact aaaaagagtt
     2941 caggatcaaa ctccacatgc aaaccaaacg tcggccacgc cagtagcgca ccgatggcga
     3001 tttgcataag cggaagcggg atctgaaagg gcatgacacg agtgaccacc ccggacagcg
     3061 agaccacgag ggtcattatc agtatggtga agaagatttc catgcgttcc ctgtttgcga
     3121 tgttttttat gatctgagaa agacgttatg cctgaattct cattttttca gaatatcgca
     3181 aaagctcaca caatgtgcgg agaaaataaa aacgggcagc cattgccacc cgttttcaga
     3241 atccgatgaa agattagata gcccagccac ccgcatagaa ggtcaccagc gccacggcga
     3301 taacgaccgt accgatgttc aacttgcgcc attcgccgga aaccagacga ccaatcacca
     3361 gagtcgcgaa gccgatcatg atgcctgtta cgatgttaca ggtcagcacg atgaataccg
     3421 ccgtaaccag acccgccatc gcatcaacaa aatcagcaaa gtcgattttc gccacgttgc
     3481 tcagcatcag caggccaacg tacatcagcg ccggagccgt tgcatacccc ggaacgaggt
     3541 aagagagcgg agagaggaac agaatcagca ggaacagcac gccaacggtg atagccgtca
     3601 aaccggtttt accgcccgcc gccgtacccg ccgcagactc gatgtatacc gctgccggag
     3661 ctgcacccac caggccagag aaaacgctgc tcatggagtc agtggtcagt gctttcccac
     3721 cgtcgataat ttgcccatct ttatccagca ggttcgcctg gccggcgacg gcacggatag
     3781 ttccggtggc atcaaatact gccgtcatta ccagcgccag aacgcttggc aggactacag
     3841 gattcagcgc gcccataatg tccaggctgc caatcagtga gttgccgttt tcatcgctca
     3901 atgaaggcat ggcgaaaacg ccggagaaat ggacgttagg atcgaagatc aaaccgacaa
     3961 ttgagatacc gataatggtc agcagaatgc caccagggac tttcagtttt tccaggccga
     4021 tgatcaccgc cagacctacc agtgacataa tcaccgggaa ggtcgcgaaa tcacccagcg
     4081 caacgggcag accatcaagc gggtttttaa tcaccagacc gacaccgtta gcggcaatga
     4141 gcagcaggaa cagaccgata ccaatccccg tgccgtgcgc cacaccgtgc ggcaagttgc
     4201 gcaaaatcca gctacggata cccgtggcag aaattaccgt aaacagcaca cccatcagga
     4261 acacggcacc cagcgcgaca ggtacgctaa tatgttgccc cagcaccagg ctgaatgcgg
     4321 taaacgccgt cagggagatg gcgcaaccaa tcgccaacgg cagattagcc cacagaccca
     4381 tcacgataga accgagtccg gcaaccagac aggttgcaac gaaaactgcc gcaggcggga
     4441 agcctgcttt acccaacata cctggaacga cgatgaccga gtagaccatc gccagaaacg
     4501 ttgttaaccc ggcaaccact tcctgacgga cagtgcttcc acgttgtgaa attttaaacc
     4561 aggcgtcgag tgaaccgccg gtacgcgctg atggcgtaga cataagaaaa catcccctga
     4621 gagtttaatt ttcgtcaaga tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc
     4681 ctgctccagg tttgcgacaa tttactgcgt cgcaaaaaag caaacgttta gctccgcgca
     4741 tacaaaacgg gtggtaaaaa aagcaaacga ttatccggcg atcatagcgg ctttttcaac
     4801 cgaagtttgc tgcttttttc tcttttattg tcttgacgat gaaaaaccag gcaattgatg
     4861 cgcaaacgtt atcgtcggtg cgcaagattg cagcgttatc gagccgtata ccgttcagga
     4921 ttgcgataaa aaatcgtctg agatgacaaa aatattgcgg ataaaaagta tacttttaca
     4981 ctggcgaaga aatgccggtg acatacaatt aaagcatcaa caccaaccgg aacctccacc
     5041 acgtgctcga atggggtgtg ttgacgtcgg gggaaaccct cctgtgtacc agcgggatag
     5101 agagaaagac aaagaccgga aaacaaacta aagcgccctt gtggcgcttt agttttgttc
     5161 atcttccagc aagcgtgcgc cggtaccttc ttctcctaag cggtcgcccg ggttacgcaa
     5221 cgggcaatca ctgcgcgaaa ggcagccaca accaatacat ccgtccagtt cgtcacgcag
     5281 cgctactaag gtatgaatgc gccgatccaa ctcttctcgc cattgggacg aaagctgttt
     5341 ccactctttc gcacttaacg tatgcccttc gggcaacacg ccaaacgctt caccaatggt
     5401 cgccagcgga atgccaatac gctgagcaat tttgataatt gcaacatatc gcaacacatc
     5461 acgtttatat cgccgctgat tgccgctgtt acggatactg gtaatcaacc ctttactttc
     5521 atagaaatgc agcgccgata ccgccacacc gctgcgtttc gccacttcgc cgggggttag
     5581 cagcgcttta atgcggggta atttcttttc cataaatcgc tttacctcaa gttaacttga
     5641 ggaattatac tccccaacag atgaattaac gaactgaaca ctgaaaagag gcagatttat
     5701 gtcccatcag aaaattattc aggatcttat cgcatggatt gacgagcata ttgaccagcc
     5761 gcttaacatt gatgtagtcg caaaaaaatc aggctattca aagtggtact tgcaacgaat
     5821 gttccgcacg gtgacgcatc agacgcttgg cgattacatt cgccaacgtc gcctgttact
     5881 ggccgccgtt gagttgcgca ccaccgagcg tccgattttt gatatcgcaa tggacctggg
     5941 ttatgtctcg cagcagacct tctcccgcgt tttccgtcgg cagtttgatc gcactcccag
     6001 cgattatcgc caccgcctgt agttttattg cccgcgcgtt aactcccgcg cgggtaattg
     6061 ctccatccat tgcataaaca cctgcggcgg catcgccttc gcaaaaaacc atccctggca
     6121 atagcgcacg ccgcgtttgc gcagccagtt aacctgctcc tcagtttcga cgccttcagc
     6181 gatcgttttt aaccccaggc tgtgcgccag ctcgatgatg tgttccgcaa tcaaatgact
     6241 ggttttgtgg gtggtcagcg tttcaacaaa cgatttatcg attttcaaaa tatcgacatt
     6301 caatgattta aggttatgca agttagagta gccaatacca aaatcatcaa ttgccacttc
     6361 gtaaccagcc tggcggaaag ccagaataat cggcgtcatt ttttcgacat caagaaatgc
     6421 atgctcagtc acttcaaatt taatttgctg cggacgcacc gcgtattgct ctgttttctg
     6481 attgattcgc gctatcaacc gtgacgtatg aaaatcggag gccgacaggt taatagaaac
     6541 atagcgatcg gcatgtgttg ccaagtacgc gcccagatcg cggaagacat tatcaataac
     6601 ataatcagtt atctgttcta tcatcccctc cttttctgcc agcggaataa actctgccgg
     6661 attcattatt tgcccctgct caccaagcca acgtaacaac gcttcagcgc cgatacattt
     6721 ttctgttttg atatcgatta ttggctggta ataaagacaa agttgatgtt tttcgagggc
     6781 gcgttgcaat ttacgtttgg gagataaata gttttgtcga atacgtagcc agagtaatag
     6841 cagaacaaga ctccccaaaa taccggcggg caacgcaaaa ataagatgat tataaaaatg
     6901 ggttataaga cgttgatatg aagtcgcaac aatggctgca attgggcgtt ttgtcgaata
     6961 aactgtcgca tataaatagc catttctttg tacggttaaa tccttcaaat gaatcagcgg
     7021 agaaaacgtt gctgccgagg cctctttgct taacgagaaa aaggttttcg tcaccgtatc
     7081 atacacaccc cattgcaatg tcgggtcatc agacatcact tcactccaga agagagggtt
     7141 gataaccgcc acataatttc cccgctgcat ataggtcatt ttatagccag agaaaaaagg
     7201 cgtatcgcgg taataataga tagaaacgtt aggttcacgc ttataatcgg ccggtgcaat
     7261 cgtatagccg tttacaggcg ctatcagcga tgagcataaa aaatggttat cacgggcata
     7321 gatcaattca ttaatataaa gatagccacg aataatattc aacattcgct tttgatgggc
     7381 tggagtgcaa acttgcccct gatagcgttc agctgcatcg ctcactaaat ctgcctggcg
     7441 aatgaccagc tcggatttat ccagtgccag ttgagcaaag gttcggagct gattattcac
     7501 ttctgatttt gcccaaagga aggcaatcca tagcgaaaga atgatgggaa agagaactaa
     7561 aaagataatg cccggcaacg ccagtaattg gtgtcgtgca cgatgactca ttgctatcaa
     7621 tttcctgcct gtttataccc ggccattgca aggtgaaaac cctgggtacc gacgtcttta
     7681 gataaatctt acggtataaa tagcactttt gcctgatttg aaattgatac ctttctaatc
     7741 gtcacctgga tgaaaataat tcattgatat tcaaaattaa tttcctacag gaatgcagag
     7801 gcggcgggaa gatatgccct taatgccact gaaaaaccac ataatctgta cagcaataaa
     7861 agtcacggcc taatatggct aacaaacaat caattgtgat atagttcaca aaattaatga
     7921 aacaaacaga gtgtttcatt tttgtgttct gcaagccccg cgatagccag agtgtaccac
     7981 gcctcccgtg aacaacgccg cgctgtccag ggcatcggct cttttcagag gaattgtttg
     8041 atggctaccc tcaccactgg cgtggttctt cttcgctggc aacttcttag tgccgtaatg
     8101 atgtttctgg ccagcacact caacatccgt tttcgtcggt ctgattatgt cgggcttgcg
     8161 gtgatcagca gcggtctggg cgttgtttcc gcctgttggt tcgcaatggg gttgcttggc
     8221 atcacaatgg cggacatcac cgctatctgg cacaacatcg agtcggtgat gatagaagag
     8281 atgaatcaga caccgccaca atggccaatg attttgacct gatacgtaga agcctccata
     8341 acggaggctt cttttttatg gctggcagat gctttaatca tccaccttaa aacaatataa
     8401 cctattgttt taatgacaaa tcagaacgga atgtcatcat caaagtccat cggcggctcg
     8461 ttagacggcg ctgccggagc ggactgctgc gggcgagact gcgcgccgcc gctgaactga
     8521 ttgccaccct gcggctgctg aggctgaccc caaccgccct gcggctgacc accaccgata
     8581 ttaccacctg ccggagcgcc accaccctga cgaccaccca gcatctgcat ggtgccgcca
     8641 acgttcacca cgacttctgt ggtgtagcga tcctggccgg attgatcggt ccatttgcgg
     8701 gtacgcagct gaccttcgat ataaacctga gaacctttac gcagatattc gctggccact
     8761 tctgccagtt tgccgaacag cacaacgcgg tgccattcag tctgttcttt catctcgccg
     8821 gtcgctttat cacgccagga ttcggaagta gccagcgtaa tgttggcaac tgcgccacca
     8881 tttggcatgt agcgtacttc cgggtcctga cccagattac caacgagaat aaccttgttt
     8941 acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc gttctgaata agtgtaaacg
     9001 cgcgattgta ccattaccaa tagcgctttt actatgttgt gacctcggtt ccgggaaaca
     9061 aacctggcca gacattgtta cacaacactc cggataatgc attccaatac tgtatattca
     9121 ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg
     9181 gcaaaaaagc gtttaatccg ggaaaggtga atggataaga tcgaagttcg gggcgcccgc
     9241 acccataatc tcaaaaacat caacctcgtt atcccccgcg acaagctcat tgtcgtgacc
     9301 gggctttcgg gttctggcaa atcctcgctc gctttcgaca ccttatatgc cgaagggcag
     9361 cgccgttacg ttgaatccct ttccgcctac gcgcggcagt ttctgtcact gatggaaaag
     9421 ccggacgtcg atcatattga ggggctttct cctgccatct caattgagca gaaatcgacg
     9481 tctcataacc cgcgttctac ggtggggaca atcaccgaaa tccacgacta tttgcgtttg
     9541 ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg acgtcccgct ggcggcgcaa
     9601 accgtcagcc agatggtgga taacgtgctg tcgcagccgg aaggcaagcg tctgatgcta
     9661 ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca aaacgctgga gaacctggca
     9721 agccagggtt acatccgtgc tcgtattgat ggcgaagtct gcgatctttc cgatccgccg
     9781 aaactggaac tgcaaaagaa acacactatt gaagtggtgg ttgatcgctt caaggtgcgt
     9841 gacgatctta cccaacgtct tgcggagtcg tttgaaaccg cgctggagct ttccggtggt
     9901 acagcggtag tggcggatat ggacgacccg aaagcggaag agctgctgtt ctccgccaac
     9961 ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg agccgcgact gttttcgttt
    10021 aacaacccgg caggtgcctg cccaacctgt gacggccttg gcgtacagca atatttcgat
    10081 cctgaccgcg tgatccaaaa ccccgagctg tcactggctg gcggtgcgat ccgtggctgg
    10141 gatcgccgca acttctatta cttccagatg ctgaaatcgc tggcagatca ctataagttc
    10201 gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc ataaagtggt gttgtacggt
    10261 tctggcaaag aaaacattga attcaaatac atgaacgatc gtggcgatac ctccatccgt
    10321 cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc gttataaaga gacagaatcc
    10381 agtgcggtac gtgaagaatt agccaagttt atcagcaatc gcccatgcgc cagctgcgaa
    10441 gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc gctgcctgct
    10501 atctccgaca tgagcatcgg tcatgcgatg gaattcttca acaatctcaa actagctggt
    10561 cagcgggcga agattgcaga aaaaatcctt aaagagatcg gcgatcgtct gaaattcctc
    10621 gttaacgtcg gcctgaatta cctgacactt tcccgctcgg cagaaacact ttccggcggt
    10681 gaagcccagc gtatccgtct ggcgagccag attggtgcgg gcctggttgg cgttatgtat
    10741 gtactggacg agccgtctat cggcctgcac cagcgcgata acgagcgcct gttgggtacg
    10801 cttatccatc tgcgcgatct cggtaatacc gtgattgtgg tggagcatga cgaagacgca
    10861 attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg cgggtgttca cggcggtgaa
    10921 gtggtcgcgg aaggtccact ggaagcgatt atggcggtgc cggagtcgtt aaccggacag
    10981 tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac gcgttccggc aaatccggaa
    11041 aaagtgctga agctgacagg cgcacgcggc aacaacctga aggacgtgac gctcacgctg
    11101 ccagtcggtc tgtttacctg catcacaggg gtttcaggtt ccggtaaatc gacgctgatt
    11161 aacgacacac tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat cgccgaaccg
    11221 gcaccgtatc gcgatattca ggggctggag catttcgata aagtgatcga tatcgaccaa
    11281 agcccaattg gtcgtactcc acgttctaac ccggcgacct ataccggcgt gtttacgcct
    11341 gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc gtggttatac gccaggacgt
    11401 ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc agggcgacgg tgtgatcaaa
    11461 gtagagatgc acttcctgcc ggatatctac gtgccgtgcg atcagtgtaa aggtaaacgc
    11521 tataaccgtg aaacgctgga aattaagtac aaaggcaaaa ccatccacga agtgctggat
    11581 atgaccatcg aagaggcgcg tgagttcttt gatgccgtac ctgcactggc gcgtaagctg
    11641 caaacgttga tggacgttgg cctgacgtac attcgcctgg ggcagtccgc aaccacactt
    11701 tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg
    11761 cagacgctgt atattctcga cgagccgacc accggtctgc acttcgccga tattcagcaa
    11821 ctgctcgacg tactgcataa actgcgcgat cagggcaaca ccattgtggt gattgagcac
    11881 aatctcgacg tgatcaaaac cgctgactgg attgtcgacc tgggaccaga aggcggcagt
    11941 ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg tcgcggagtg cgaagcatca
    12001 cacacggcac gcttcctcaa gccgatgctg taatcgttaa ggccgctttc tgagcggcct
    12061 tttcctttca gagttgtacc agcaatttac gcttttcttc cggtagtaaa ttcactgcct
    12121 gctgataaga cgcatccacc agataataga tttgcgaatc cggcagcgaa ccgtcgagat
    12181 acacggtgct ccagtgcgct ttattcagat ggcggctagg acgcacatcg ctgtgctgct
    12241 gacgtagcag ctccgccagc tccgggctgg ttttcagcga aacagctggg cgattttcta
    12301 cttctttcac catcgcaaac agtacatctt ccactttgat ctgcgtcgct ttccagtcgt
    12361 tatgcacgct ctgttctgcg cctggttttg ccatgcaata ttgtagcaac tccgaaatgg
    12421 tcatttttta ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat
    12481 gttcccccag ccagatgcct tgccaggtgc cggtctgaat acgcccttta tgtaccggca
    12541 atacaagcga tgttcccagc attgaggatt tgatatgaga aggcatatcg tctgctccct
    12601 catagtcatg ctcataattt ccgttgtcgg gaacggtgcg gaggaaaaaa cgctccatgt
    12661 cgtggcgtac ggtgggatcg cagttctcat taagtgtcag agaggcggag gtatgttgca
    12721 gcaacagatg cagtaagccg atgttaacgc gcggcatatc agccagctga ttcagaattt
    12781 catccgttac cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc
    12841 acatgtgctg ctccttgata aaactctctt aatcagtttg cagcaagaca gcgaaaggat
    12901 aaaggtgtga ttaaaaaaac agcattcggg agagcgtcac gctctcccgc tctgtcagta
    12961 ttctgaattg acgatcacct cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt
    13021 agagttggag gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg cggcggtaat
    13081 atcattatca gaatcgccat aaaaaattcg gatattttta tcctgcagcc attgcgattt
    13141 tgtattttgc cctggtttat cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat
    13201 atgaaaatta tccgccagcg tttttgaaac cgtttctgtt ttcgtcggac tacgaccagt
    13261 cacaaagaag atcgcgtcac cgcggcgtac atgcatatca atcagctggc gagcgacctc
    13321 ttttggaatg ctgaattcat cccagccatt gttcattttt tcccagaaca caggattttt
    13381 cagataatct tcgctttctg gcgagaaggt ttttttgccg cgccagaagc ccggactgga
    13441 aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc ctgcgaggct
    13501 attttcaatt tgtgcgaccg aaacccaatg aatgggtgcc tgttcagcaa gcctggcaac
    13561 gttagtccca gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg aactgtttag
    13621 agcaaacaat aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat ttttccctaa
    13681 atagtcagta tgttgaaact ttttattgtg agatttgttg caaaaaccat ctgaccataa
    13741 cgccagctgc cggggaaagg aaggattatc tgcggttttt gtgatgaaaa gcaggatgat
    13801 tatatttaac ccagtaaata cgttcctgtc tttttttgta taaaaaatgt ttattactcg
    13861 atgtaaggat acattaattt tacgtttatt atgatgaatc ctcggcaaat gagcgaaatt
    13921 tgatagaaca tttatctgct gtacgattta atgaacttaa tgatacagca ttacaaaaac
    13981 aatcgaagtt tataaagatg atttctgatt gaccagcccc ctaccctaca atggtgatta
    14041 attttattac
//
LOCUS       DAZFWP020000126        13950 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFWP020000126 DAZFWP020000000
VERSION     DAZFWP020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.126"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..688)
                     /locus_tag="ACHK0Z_RS18290"
                     /old_locus_tag="pgaptmp_003658"
     CDS             complement(<1..688)
                     /locus_tag="ACHK0Z_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4503613.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWH"
     gene            complement(699..1397)
                     /locus_tag="ACHK0Z_RS18295"
                     /old_locus_tag="pgaptmp_003659"
     CDS             complement(699..1397)
                     /locus_tag="ACHK0Z_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4503614.1"
                     /translation="MQDIHEETLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1397..1726)
                     /locus_tag="ACHK0Z_RS18300"
                     /old_locus_tag="pgaptmp_003660"
     CDS             complement(1397..1726)
                     /locus_tag="ACHK0Z_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4503615.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1723..4368)
                     /locus_tag="ACHK0Z_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             complement(1723..4368)
                     /locus_tag="ACHK0Z_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4503616.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEY
                     KRQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQR
                     LRDVYGDNPAALAKATSALKNTWSAEEQLRGSWLAGLKSGWGEWAESATDSFSQVKSA
                     ATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAF
                     GGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGN
                     LYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYD
                     MARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(4418..4720)
                     /locus_tag="ACHK0Z_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /pseudo
     CDS             complement(4418..4720)
                     /locus_tag="ACHK0Z_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(4747..5169)
                     /locus_tag="ACHK0Z_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             complement(4747..5169)
                     /locus_tag="ACHK0Z_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4503617.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQKTTKHN
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKVS"
     gene            complement(5183..5935)
                     /locus_tag="ACHK0Z_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             complement(5183..5935)
                     /locus_tag="ACHK0Z_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4503618.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(5943..6341)
                     /gene="gpU"
                     /locus_tag="ACHK0Z_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             complement(5943..6341)
                     /gene="gpU"
                     /locus_tag="ACHK0Z_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGU4503619.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(6354..6977)
                     /locus_tag="ACHK0Z_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     CDS             complement(6354..6977)
                     /locus_tag="ACHK0Z_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4503620.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(6980..7261)
                     /locus_tag="ACHK0Z_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     CDS             complement(6980..7261)
                     /locus_tag="ACHK0Z_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4503621.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(7254..7580)
                     /locus_tag="ACHK0Z_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             complement(7254..7580)
                     /locus_tag="ACHK0Z_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HGU4503622.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(7668..9647)
                     /locus_tag="ACHK0Z_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             complement(7668..9647)
                     /locus_tag="ACHK0Z_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4503623.1"
                     /translation="MKNKSWFRMLAGSQGEADIYIYDEIGFWGVTAKQFVSDMNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(9637..11139)
                     /locus_tag="ACHK0Z_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             complement(9637..11139)
                     /locus_tag="ACHK0Z_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4503624.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTEQQRQQATDSQHEE"
     gene            complement(11139..11351)
                     /locus_tag="ACHK0Z_RS18355"
                     /old_locus_tag="pgaptmp_003671"
     CDS             complement(11139..11351)
                     /locus_tag="ACHK0Z_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503625.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(11348..13471)
                     /locus_tag="ACHK0Z_RS18360"
                     /old_locus_tag="pgaptmp_003672"
     CDS             complement(11348..13471)
                     /locus_tag="ACHK0Z_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4503626.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(13468..13944)
                     /locus_tag="ACHK0Z_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             complement(13468..13944)
                     /locus_tag="ACHK0Z_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU4503627.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2918 a         4507 c         3310 g         3215 t
ORIGIN      
        1 cgtgccagtg gcggtgacgc cagacagaat gcgttcgtcg ttgccatttt tcggaatacc
       61 tctcccgttt actcagttgt tcaggcagat ggtgaagcag ttcaccgttg ccgcagtata
      121 tggcggcatg attggccacc gatgcgccaa agcagcacag caggatatcg ccaggctgtg
      181 cggaaggcag ggaaatcctg taaaaaccag tcgcctccat attgtccagg tacaggttct
      241 gaccattgcg ccaccagtca tcctcacgct caaaatccgg catatcaatt cccgccagat
      301 ggtaggcatc ccggaacagc gtgtaacagt ccgtcacccc gtgctcaaag cgccgtcctg
      361 tcagatgtgg cacacagcgg aatttatgaa tcgccccacg gcagaccagc caccacggca
      421 aatcactctg cacctgcagc cgccggtcag cctcactcag ccagggcagg ccaccggggt
      481 ggctgtggac cagcgccaca atctcaccct gcatctctgc ccgcagccag tcttccggtg
      541 caatacgaaa atacgcctcc ggctctgcag agatattcac acaagggata taccgctccc
      601 cctccggcgt tctcaccacg aagccgcacg actccgcagg cgcacaccgc cgggcatgcg
      661 ccagaatcgc tgattcagtc tgtgtcataa accgggattt actgcgaaag tttattaatg
      721 gaaaggaaac cgccaaaatt agccaccatg ccgcgcatct cacacccgcg catgcacttg
      781 ctgcatctgt ccttacggat atcggtggtg gggttgtcga actcatccgc caccgcagga
      841 ccgttatacc cgcattcatc tccccggtaa tcccacatac aggtgttcgc cagcatgatg
      901 cgaccaggaa acagcgcccc gtccgtctcg gtcggtgtcg ccagcacaaa cgaggccgtc
      961 atggccgtca gctctgacat ctgctccacc acccagcggt cgctcagctc ctgctccggg
     1021 tccgcctccg gattgcccgc aacgaaattc accgcatcca gaaaacgcgc atacacccgg
     1081 cggcggacca ccgtggcacc caccaggctc tgcaaatcct ccgccatccc ggtgacaagg
     1141 ccgaaaagat tggacactgt cagcgacggg cgggcactgc tgccctttcc gttcatctca
     1201 aagccactcc cctcaatcgg gtatgcctga tattcacgcc cctgccaggt caccggctcc
     1261 cctttttcat tcagctcatt gcagaaaaaa taccgctcac cgccctgcac cgtcaggtcg
     1321 atttcccaga gcaccacccg cggtgactgc tctgacttaa ccgactcgtt aagagtttct
     1381 tcgtgaatat cctgcatcag ttcaccacct gcttaaactc cgcgctgaac tcaacgcgca
     1441 acatcccgac ccgcgcagac cacccggcac aggtcacctt tatctgccgg tatgcatagg
     1501 gtggcttcca caaaaatgcc ttccagcccc cgtgctctgc cagaaacgct tccagatgcc
     1561 gggcctcctc ccgggtcacg gaaagcgtca cccggtatgt tttcaggtca gcattcagcc
     1621 ctgccgccat acgctgtgag tacccgtcac caaaacgcac ttcacgcacc gatggctgcg
     1681 agttcacctc catatccggc ttcactttcc agcgaaatgt tttcatcgcc cgctccctga
     1741 taacataccg ccatcacgca actgcagccg gagctcatcc tgcgccccct tgcgggccat
     1801 gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttggtgccgt cgttctgaat
     1861 caccacgtga ttgttctgat taaaattaat accttccgcc cgccgcatct gcgccggact
     1921 tccggcacca cccacataac caccttccgc atagccgcgc atcagacggt aaagattccc
     1981 cacacctatc cggctggttg cctctttcgt gaaaacaaac tccccgcggt gaactatccc
     2041 cgcaggctca tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat ggaagttcgc
     2101 cgccgcagcc tcaatggccg tccccgagga agcagatgca ccaccaccga aagcaccgcc
     2161 aatggcgctg ccgatacgcc cgacaatgcc caccatggcc tgtttaagca ggatttctgt
     2221 catcatggac agcaccgaac gggtgaatcc ccgccagtct gcctctgcac cggtcagcat
     2281 cgccgccata ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt taacctgcga
     2341 aaaactgtcc gtcgcacttt ccgcccactc cccccagccg gacttcagcc cggccagcca
     2401 gcttccacga agctgctcct ccgcagacca ggtgttcttc agtgccgatg tggccttcgc
     2461 cagcgcagcc ggattatcac cgtacacgtc ccgaagacgc tgcgcttcag actcccgctg
     2521 cgcctgacgg tcagtgagac cgcgggcttt tgcgctgatg gcggcctgct tcgcgctctg
     2581 ctgctcttca aaccgcaccg cctgctgtgc cagctcattc aggcgtttct ggtattcaac
     2641 cttgtcgccc agctcagcca gctggcgttt gtactccagc gtctcgtctt tatgcgccag
     2701 cagggatttt tcctgctcag ataactgtcg tttcgtggca gcctctttca ggaccgcata
     2761 ctgactttcc gccttccata aatcacggcg ctgccggctg attttctcat tcgcaccgct
     2821 gtgtttttcc agcgtcctga gctcagcttc aagggcaagc agagcctctc ttgcctgctc
     2881 ctcttccctc tccccggcag agcgcgtttt cggtgatgta tgctttttac ctgtcagctc
     2941 ttcagccaga cggctgacgg cttcctgctg ccccggacct ttgctgacgc ctgttgcacg
     3001 cgaacggttg atgtacccca tttccccctg gcgtatacgc gcatcccgtt ccgcaatgga
     3061 ttttctcagc gccagttcat cgcgttttgt tttctcaata aatacgcggt tctcttctgc
     3121 cagttcacca aacaacgcac caacgccggg cacattcttt gtcgtttccc aggctgactg
     3181 aataaattca gccagcgcca gatccccctg cacaagcagc agcttcacct gttcaacggt
     3241 tcctgccacc acgtcagtga tcagactgag tgcccccagt gtatgatcac ctatccatgc
     3301 ccatgcgtca gaagtccagg ttttaacatc gtcccagatt ttttccaccg gcgtggccgc
     3361 tttatcaagt tgctccagac gtgcattcat gacatccgca aacagggaca tcgcctccgt
     3421 caccgcagcc tgtttacctt tcgtgcgctc aagctcatca atatggcgta actgggaaac
     3481 gctcaggaag ttatactgct gattcaggga ggccagcgcc ttcaccggat ctgctgcaat
     3541 cccttcaaag gctttttcca ccttcccggc atcgtccccc accgtctgca gccatctctg
     3601 agaggtttcc cccatgatcc gtagctgccc ggcggtatat ttcccgcttt ctgccagacg
     3661 ggccagattt tctgccgcct gtctgatacc accaccggct tcatcgctga tcaccccggc
     3721 cattttccac aattctgccg ttgtggtggc agcagcccct ccggtcaggg tcagtgaacg
     3781 caataaggcc cggtcagcct gctctgcctg ccaggcggcg gcagcaagcg cggccagtac
     3841 ggcaaccccg ccacctgccg ccacacgggc caccgacata aatcgtccca gctcaccggc
     3901 attccgggca ttttcagcca gtgcatttgc cgtatctgac agcgactcct ctgatgattc
     3961 agaggcatcc ctgatcccga gaagttcctc cttcagcagg gtaagcaggc tgagcggtcc
     4021 accgaatgaa tcgctgatct gccccccctg ctgcagcatg ataaggaagg gattctgacc
     4081 accggcaagc tgagtgacaa tatccgtgaa ctgtgcgggc agtgtgcgca tggcagcctt
     4141 atactgtccg actgatatcc cggctttttg tgcagccagc gcctgtcggc tcagactctg
     4201 ctcagcagca tcagcctgtt ttctgaaaga ctgactgact ttcccggaca tcaaatcagc
     4261 aagcgcactg gtttcaccca gctctttttt tacccgcgct gcctcttcag aaaaacgggc
     4321 agaatccagt gtcagcacgg ctgtcagatc ggcaaaatta cctgccatcg tggtcacctc
     4381 ctgaaatgtc ctctgatacc atcaataact gtcacaacct ccttccctcc tccccgaaac
     4441 ggactccacc ggcgaggccc gccgccttct gcatcagtac atcattttcg tccggcgtct
     4501 ccgcctgtcc ttcctctgcc tccggagcga acaggctgaa atccgccgga tgcatatccg
     4561 gatcgcaaaa aaacaggctg agtacggcgt acgtcagccc ggaaaaatgc atatccagct
     4621 gggtatcctg aaaataatgc gtgcagtaaa aacgtcgcca gtcggcatat tcggtggatg
     4681 tcatcccggc aagcatggcg cgccagtcgg ctctccccat ctcacgcgcc agtttcagga
     4741 caaagttcag ctcaccttcg aatgcttttt tgatgttacc ggctctgtcg cttctgcttt
     4801 cccggttggt tcaggatcgg catcgtgccg gttatccagc atacctgaaa gataaagcac
     4861 ccggttcgtt gcctgattca gtgcatcagc aggccatccc agcatcactt cacggcggat
     4921 ctgctgcatc tctgtctccg gagaggccag agtgcctttg agggaatggg aatgccatag
     4981 cgacatcgcc acaagcaggg atgccgtttc cagatatcgc tggttaatgt gcacggcatt
     5041 atgcttcgtt gtcttctgtt gtgctgcgtc tgaaacaaac ttcatataat caaaccgctg
     5101 cagcgcagac aattcggaaa gcgtgacgga cacaccgtta tattcaaatt gttctgtttt
     5161 cagaaacatg tattacctcc gtttaccctg cagcgcccgc ttcagtaacg gtgacttcag
     5221 ccactgcggc gaactgacca tttccgctca ccacagggat ctgcacctta cctgtcgcca
     5281 cgccgtttac cgtaattgtc atatctttca cactaatggt ggctttcgac ggatcggcgg
     5341 aaaccgctct gaacgtcttg tcggttgcac tttccggctc aaaagaaacc gtcagggtgg
     5401 ttgttttccc ttttgccacc gtaccggatg tcggtgtcac cttaattgca gtggccggcg
     5461 taatttcgct gcgttcttcc gccacggaag gtttgcccac gttagtgact ttcaccgtgc
     5521 gggtgatcac ttctttcgcc gtcacggcct taccgatact gctgacccag ccacggaaca
     5581 catccaccgt gccatttggg aaacggattt tataggcccg cacatccccg ctttcaaacc
     5641 agcctataag ccctttctga ccttcttctc ccggtttcca ggccagcgta aaactggtat
     5701 ctcctgcaga cttctgcccc tgcccggtcg cggtccagtc cgcgtcttca tcatccaggt
     5761 agttatcatc gtagggttct gccgtcatct cgcccggcgt cagatccttc accttagcca
     5821 gtcgctgcca gtcatcgtct gacaacgggt ttgcataagc atcacccttg ccgttgtaaa
     5881 cccacagagt ggtaccggca ccttttaccg gctcaagggg atttggtgtt gccatatcgt
     5941 cctcacatct cgtaggtaat tttccacagg agatctgccg atccccacat cataaactca
     6001 tcatcccggc ggtagtcata cccctgaaga ttcatcttca gcagtaatgc actgagcccg
     6061 ggaactgcct ccagcgcagg aagaattttt tcttccatcc acatatccag tgccgagtcc
     6121 ggttcttttg ccctgagaaa aacctcaata tgcagtgtcg cctcccaggt tccctcatca
     6181 acgaactcgt cagcagcaga cgcatctgtc aggtaaacag caacagcagg cagttcctgt
     6241 tcatcaataa aaaccgggcg accgtcaaac cagctcaccc tctcagaaat attttctttc
     6301 agggcagaca gaactgccgc ccgtatttca cggtgtttca tacaccctcc cagtcatttt
     6361 cttttcagca ccaggcgtaa ctgatgcgtc atggctttca tcatctgcgc cggtaatttt
     6421 tcccggtaca tccggtcccg ttcacgttca aaggtttctg ccagcggtcc ggcagtcgta
     6481 atcttcacca cttcgatcgg cagacggtgg cgtttcggcc tccctttgct gtccgcgccg
     6541 gtggatgatg atgcccacgg catacgctgc atcacatgcc agcgtccgtt agccagccgg
     6601 gtgataaagg cgtccgggat ccgtcttttc cccacaatca gcacactgcc gccccctttc
     6661 agggccgcac gctgtccttt ctttctccgt tttctgcggg aaagtcgaac gcgggcctcc
     6721 cccagtttga tggcgggcag gttgccggta ttgatgtaaa cctttgcgta aaccttatcc
     6781 ggtcgtgccg gacttaaccg gatgcgggca cggataagac gacggggaac ggccagctcc
     6841 ctggcaactg aagaggccgt tttcgcaata atggcccccg ccacgcggtt cagtgtcgtg
     6901 gcagaggccc ggggaacggc acggcgatca attgcatcca gatttttcat ggcctgcgcc
     6961 agacctttta ttgccatact cattcctgtt cgacaaaaat ccggggttta ccgttgtacg
     7021 tgtcataacg ggtcaccgtc agtgtgcgcc ccgcaaacac aacaacatca tgacgggccg
     7081 gacggtaccg ggctgaaaac accaccagtg acaactggct gcccgaaagc gcccccatct
     7141 ccgcggactc ttcctccggc atcacgtcgt acacgacgcc gttaatctcc gcctgtttgc
     7201 ccatcatccg aacggtcgcc acgtccatcc ggcaacacat tcgcgtaaac agatcagaca
     7261 ttgattttta ccgccacagt ggcgctgttt gcaggagcat tttcccaggc tacccccgcg
     7321 gccaccgcac cgtcggcagc cagctgcaca accccgtcct tcagataaac caccgcgccg
     7381 gactgaatgt cgtcagcaga ctgtttgggc agcaggaaca cgccttcggc aaaaccgtca
     7441 ccggcctcac cggcaggaat atcggtaatg gccacggcca ccatactgcc gaccaccacc
     7501 gcagcaccgc tcaggatggt ctgatctccg gcattcacca gttcaatggt ggtaccgtcc
     7561 tgtacaaaat ttttcgccat aatgctgttt ctccggacag cccctgtggg gctgtttttc
     7621 aggcataaaa aaagcccttt cgggcagtga ttgtgataac gcggttatca ggccaccgac
     7681 gaacgcacca gcccgcgcca gtcaagtggt gccactccgg catcaatacg gatttttgtg
     7741 gcaatgccgt cagtggtgaa accttcctgc tgatcaatgt atggcgtgtc cacaccatcc
     7801 agccaggcca cttcaatggt gtcagtgccc tgtgccgccg ccagatacca ggttttcggg
     7861 tctgccgcat caagacgcgc ttctgcaatc acctcagcaa agttctggat agggttaatg
     7921 acaccggcgt ttgcatccgc ccctttcaca ctggccgatt tgatggtctg gttcgccacc
     7981 gtctccagtg ccaccggtac cagcataaag gccggacgga tattcagggc acgatcgcct
     8041 tctttctgca ggcgcatcat ctgacgggcc gcatccagtc cggaaacgga gatccccccg
     8101 gtggcaatat ttttgtgatc ggcatggaac agcgccttac cgtcggacag tttcgggtta
     8161 tccgtcagca ccttgtagac caggtcacca atcgttgcct tcgccgcacg ccccatcttc
     8221 atcggcacgt ccaccagcat attcagatca tcattgataa tggcctggcg ggtgatggag
     8281 aaaatctccc cgtaagtggc cagtgcaatg gtctccttgc gatctgaggt ggtgatgtat
     8341 ttatactccg ccccctcacg aacctggcgc agagaaccaa aaccgcccat ccccacgcga
     8401 tacgctgttt tgaagtctga caggcgtccc ttacgggtcc acttctggaa ggtttcttct
     8461 gattcctccc agccctggat cagccccttg ttcgacacat ccagcagaat attgccaaaa
     8521 tcagaggtgc tgtgcgtcag cgccagcccg accatctgca tggggttata actggccacc
     8581 ccaataccgc gctccgtcag tgacatgcga gcccattcac gcagggtcat cccgttatag
     8641 gcgttatcct tctcgacatt ttcaaatccg gcacgggcca gcatcgcctg gcggatcccg
     8701 tcccccacaa aattgccgtt tccggcataa atatgagccg gtgtattttt gttggtcggc
     8761 gaggactcct tgcccatttc attcagcaga cgttcacggg ccatttccag cgaacagtca
     8821 ggatcagcca cgcactgtgc ctgaagcgtc tgatagcgac cgccgaacat ggcaaacaga
     8881 tcgttaatgc ctgacatgcg ggctttctgc tcagccataa cgcgggcgcg aatggtcgcc
     8941 tcatcagaca ctgccggtac cggtgatggt tctgttaccg ccggtgcagg gattgtcact
     9001 gtggtatcac gcggggcact gttgcgtggc ggagtaatca tgtttcggat ggattccggc
     9061 atctttttaa attcctctgt acgttttgac tgaatacatg ccattgcctc aacagcgggt
     9121 gtcacctggt cagcaaatcc gtgtgccaga cattcggcac cggacatcca ggtttcatcc
     9181 gccagcatgg cggcaatttc atcggtggtt tttccggttt tctgcgcata ggctggcaac
     9241 agtaccgatt cgactttatc cagcaaatcg gcataactgc gcatatcctc agcatccccg
     9301 ccactgaatc cccatggctt atggatcatc atgaaggcat tttccggcat aatgaccgta
     9361 tcaccggcca tcgcaatcac agatgccatc gaggcggcaa cgccatccac atacacggta
     9421 atggtcgccc cctgattttt cagggcatta aaaatggcga tgccttcaaa gacatcgcca
     9481 cccggtgaat tgatatggag attaatgtgg gtgatatcac ccagggcatt catatcgctg
     9541 acaaactgct tcgcggtaac tccccagaaa ccaatctcgt cataaatata aatatctgcc
     9601 tcaccctgac tacccgccag catcctgaac caggatttat tcttcatgct ggctgtcggt
     9661 ggcctgctga cgctgttgtt cagttccggc actgttgcct cctttgtcgt tgacggggtc
     9721 agtatcaaag accagcccca gtctgctgtt ttcatcaatt tcagccttgc ggcgacgttt
     9781 gacctcatcc ggattgcgcc caccagcacg cacccagtca gattctgtcg ctgcaccacc
     9841 ccggatctga attctccagg cttcagcttc cttaaccggg tcgatccacg gcatcaccgg
     9901 accggaatac gtcgcgttat atagcgtttt catctccaca tccgccggaa ttttcagcag
     9961 acctgccgca accaccatat tcagccatgt ccggtacacc gggcgggtta ccgcgccaat
    10021 aaaacagtcc tgcaggatca ggtaaccatc cgtggactcg accagctcct gccgctgggc
    10081 gctgtaggtg ccgttatagt tacgcgccgc actggaaaaa ctcagacgac tgcctgctgc
    10141 cactgcacgc aactggccgt tgcggaaagt ttcaaggttg ggattgggac ggtcagattt
    10201 gaccatgccg atatcctcgc ccttgcgcaa atcgtcataa ataatacccg gggtgatatg
    10261 gacttcccgc tcggtttctt tgatccccgg atcttcatag tcctgtccgt cacctttacg
    10321 gatatacagt cccagcgccg cagcaatacg cgccgctgtc agttccgcat cctcatactc
    10381 cttaagggca ctgatccgca tcagcacccc cgataacatg gatgagcctc gcgtctgatg
    10441 cagacgacga gtgaacttca ggtggatcat ttttccggca gcgatttctt tcgtatcact
    10501 ctgccggccg ctgaccggat aatttttata aaccagatat tttttcggtc ttccccactc
    10561 atcaagaaaa accccctgat tcagtccggc ggattcatca gtgcgcatgg gaacaaaatc
    10621 cggctccatc gcctcaagcc agaatggcac tcccgccgtc cgttccagac cgtttcccgc
    10681 accactgacc atctgcgcaa acacttcacc atcccgcagc caggtccgca gcagtaaacg
    10741 ttcaagcaca ggacgggtat actgccctgt cacatccgga ctcacggacc attcagccca
    10801 caaacggcgg atatccgcag ccagctcagc cgccatttcc ccgttttttc gtaatggctg
    10861 aggctccaca ataattcccc tggcaccaat cacccgctct tccagcttgt caaacacacc
    10921 aatcaccagg tcatgattga tatccagaaa acgggcctgc tcccgcaggg aaaccgcacc
    10981 gtatttactg agctgatcag cagagcgatt ttcccgccgg gctttatgtg tccgggtcgg
    11041 tttcaccgcc tcataggcca tgattaacgc ccttgaacgc agtctggctg ctttccaccc
    11101 gggggaaaac acgccgatca catcatcaat aattgccatt aaaacctcgc cagtttaaat
    11161 cccggttttc cccgcctgcg gctcaccatc gcggcaagcc tgcgttccca ctcctgacgt
    11221 ccggcgcgga tctgagaaag gctttccagc gtcagttgct gcccgttgaa ggtgacagac
    11281 tttccctcca gtacggccat ttccgcttca cggtaccgct gtatcatttc tctggcttct
    11341 tctgtgctca caaccagcct cctgatgtta tccatggatt atcttccgca cgctccgtcc
    11401 gcagtttttt cttccggcga cggcgttttt ctgccccggc cgtcagttcc ggggataccg
    11461 tttcaccaga acgctcctgc gggaagacga gccacgtttc ccgctgtgcc cagtccggtg
    11521 cggagggcca gcggatcttt tcgtaaccat gcagaacggc aagcgcatcc gcataaacca
    11581 gcaggtcaaa cgcttcgtta gcgcccctgc ccggttttcg ccattttccg tcactgccgc
    11641 gctcttcata ggtcagctca tcgtaaaacc accgccccag ccagtcggga aagtggatat
    11701 agttcggccc tggtgtgtca cgccacaggg cattatttac acgatcctta aacgcatccg
    11761 tctgaaccag ccacagcgcg acatcgccac tggctctggc acggcgggca cttctgccgg
    11821 tattatccgg gaaggttcgg ttaatcagcc tgtcacggcg aagtccatcc cccttgaaca
    11881 gaaacaccct gttgcccagt ccgtcactcc ggcaacgacg ccagaaacga taggcgttat
    11941 ctgtcacccc ggcttcccct cccgtatcca ccgccatggc catcagacgc atgcgcacat
    12001 ccggatcaga agccagcggc catgttttat ggaacacatc cgtcagcaac aaatcccagt
    12061 cctccggata tgccgccgga tcaaccggca gactttcacc gttgggactg cagcgcagtg
    12121 aatgccggat gttgtagcga tcaacaatcc agcgttcccc ctgctctccg tatccggtga
    12181 tctgcacaac aaaacggcga tttttaccgc cctgtacgtc aaccgttgcc tcaataaaac
    12241 gcacaccatc cggcacagat cgccggggaa acggctcggc acgctgttca agcagttcac
    12301 ttttacgctg ttccgtggct gaacggggca gatagggtcg tccgatatcg gtgttcacca
    12361 ccgctttcag ggtctcttca ctgccggttc gctcatactc ttcttctgcc gccagcagtt
    12421 taaaaatcag ttgttcccag gtctgaaacg ccgcagctgg cccctccatc caaaatgacg
    12481 caatccggga gtttcgtggc gttccggtga tactgccgtc cgccgccgcc cgttcacctt
    12541 cacgaagcca gatcccctgg ttattcagtt cgcgtttctg ctcaggggca atcagcccgc
    12601 gacaatgcgg acacatcaga cgggcagcct gaccggcagc cacaaaatcc gggttattcc
    12661 ggtatccggt catgttatcc atcaccggct gaaaatattc cccgcagtgc ggacacggcc
    12721 agtaccaccg gcggcggtct ccccggttat acagtgacag gatacccgtt gttggcggtg
    12781 cctcatgtgc gccaccacaa cgccatttgg tatcggtgat atcccgcccc ggtgaactct
    12841 cgaccagggt catccccgag gacataaagg tggtggtacg ctttgaggcc agcgtgaagg
    12901 catccccttc cccgtccacg ttttcaggga aacggtcata atccgtcagc gccacacgac
    12961 ggtaatccga agaggaaaag acggtgatcg acggccagcc aatcttcagg aaggagccgt
    13021 caagaaacat tttatcgtgg acgttgttgt cattacggga aggactgagg cgcttgctga
    13081 cctccggact gtggcgaaac gtcctggaaa gacgcgttct ggaatgctca cgcgccttcg
    13141 tctcagtcat ctgcaccacc agcatatccg ccggatcaca gatgatgccg tacacaatcc
    13201 agccatcaat cagcccttcg gttttcccgg ttcgcgcagg tcccacaaac accaccgcgt
    13261 catattcacg ggctgataat gtattaatgg ggtcaatcat atagggcgtc agcgatgact
    13321 cccacggacc ggaagtattg gctccccgtg gaacccgcat ataacgcctg atggcttccg
    13381 ctactggtaa ccggccaggt gggcgaaaca gcgaggccac ttcgcgccag atatcggatg
    13441 cgcggctatg gctctcgttc acctgattca catatcggcc tcatcacaac agtcaatgac
    13501 tgccttttcc agtgtgtcgc ggatctcatc aaccacaatc tgtacttcat tcagttgtga
    13561 tgcggtccac cccctgtccc tctccagccg gtcaggccag gtttccagta cctgaactat
    13621 cgctttcacc acgacagaaa aggaccgcct gacatcactg actggcacaa gctgaccagt
    13681 ttcctgctca aatttcagtc ggtcacgttc tgactggtac catgctttac gcgcatgagg
    13741 atccatttcc tcgttatcta caggcagagg agctttcatc agctcggcaa ggatatctgt
    13801 cagtcggtac agtttgagat tgctctcatg accaccggct gggcttatgt tttttacccg
    13861 agccgcaaca gtctgtcgat gagcaccgga taatgcggcc agttgggaaa tattcagatg
    13921 cagatttttt aattcacgat ccataactcc
//
LOCUS       DAZFWP020000127        13863 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFWP020000127 DAZFWP020000000
VERSION     DAZFWP020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.127"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            26..325
                     /gene="yoaC"
                     /locus_tag="ACHK0Z_RS18370"
                     /old_locus_tag="pgaptmp_003674"
     CDS             26..325
                     /gene="yoaC"
                     /locus_tag="ACHK0Z_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU4503628.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(445..624)
                     /gene="yoaH"
                     /locus_tag="ACHK0Z_RS18375"
                     /old_locus_tag="pgaptmp_003675"
     CDS             complement(445..624)
                     /gene="yoaH"
                     /locus_tag="ACHK0Z_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU4503629.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            698..2059
                     /gene="pabB"
                     /locus_tag="ACHK0Z_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             698..2059
                     /gene="pabB"
                     /locus_tag="ACHK0Z_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU4503630.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2063..2641
                     /gene="nudL"
                     /locus_tag="ACHK0Z_RS18385"
                     /old_locus_tag="pgaptmp_003677"
     CDS             2063..2641
                     /gene="nudL"
                     /locus_tag="ACHK0Z_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU4503631.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2825..4189
                     /gene="sdaA"
                     /locus_tag="ACHK0Z_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             2825..4189
                     /gene="sdaA"
                     /locus_tag="ACHK0Z_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4503632.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPLKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4320..5918
                     /gene="pdeD"
                     /locus_tag="ACHK0Z_RS18395"
                     /old_locus_tag="pgaptmp_003679"
     CDS             4320..5918
                     /gene="pdeD"
                     /locus_tag="ACHK0Z_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4503633.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5922..7478)
                     /gene="yoaE"
                     /locus_tag="ACHK0Z_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             complement(5922..7478)
                     /gene="yoaE"
                     /locus_tag="ACHK0Z_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU4503634.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWGGVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7466..7624)
                     /gene="yoaL"
                     /locus_tag="ACHK0Z_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             complement(7466..7624)
                     /gene="yoaL"
                     /locus_tag="ACHK0Z_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU4503635.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7941..8912
                     /gene="manX"
                     /locus_tag="ACHK0Z_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             7941..8912
                     /gene="manX"
                     /locus_tag="ACHK0Z_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU4503636.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8975..9775
                     /gene="manY"
                     /locus_tag="ACHK0Z_RS18415"
                     /old_locus_tag="pgaptmp_003683"
     CDS             8975..9775
                     /gene="manY"
                     /locus_tag="ACHK0Z_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU4503637.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9788..10639
                     /gene="manZ"
                     /locus_tag="ACHK0Z_RS18420"
                     /old_locus_tag="pgaptmp_003684"
     CDS             9788..10639
                     /gene="manZ"
                     /locus_tag="ACHK0Z_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU4503638.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10694..11152
                     /gene="yobD"
                     /locus_tag="ACHK0Z_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             10694..11152
                     /gene="yobD"
                     /locus_tag="ACHK0Z_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU4503639.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11581..12147
                     /gene="mntP"
                     /locus_tag="ACHK0Z_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             11581..12147
                     /gene="mntP"
                     /locus_tag="ACHK0Z_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU4503640.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12144..12953)
                     /gene="rlmA"
                     /locus_tag="ACHK0Z_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             complement(12144..12953)
                     /gene="rlmA"
                     /locus_tag="ACHK0Z_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU4503641.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13119..13328)
                     /gene="cspE"
                     /locus_tag="ACHK0Z_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13119..13328)
                     /gene="cspE"
                     /locus_tag="ACHK0Z_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4503642.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13341..13484)
                     /gene="yobF"
                     /locus_tag="ACHK0Z_RS18445"
                     /old_locus_tag="pgaptmp_003689"
     CDS             complement(13341..13484)
                     /gene="yobF"
                     /locus_tag="ACHK0Z_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU4503643.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3418 a         3470 c         3454 g         3521 t
ORIGIN      
        1 catggttaat tttaaggata agaatatgcc tgctgtaata gataaagccc tggatttcat
       61 tggtgccatg gatgtatcag cgccaacacc aagttcgatg aatgaaagca cggcgaaggg
      121 aatctttaaa tatttaaaag aactgggagt acccgcgagt gccgctgata ttacggcgcg
      181 agctgaccag gaaggctgga accctgggtt cacggaaaaa atggttggat gggcaaaaaa
      241 aatggagaca ggtgaacgct ctgtgattaa aaatcctgaa tacttttcaa catatatgca
      301 ggaagaacta aaagcactgg tctgagttaa atttatatca gcataaatgg gtcaggacgc
      361 ttttaatcac atataaaaag cgtccttttt ctcacaatca aatgatcatc ggcttctata
      421 acgaaatcga tacggattag tgtgttactc gtcttcatcc tcaaaacgcg ccacgattcg
      481 ctcgccggag tggttggcac gcagctcttc tgccaccagc gcgattgcct ggccgctgct
      541 catcccttgt gccatcagtt cctggatccg ctcgacagct ttttgctgct gttcatgggt
      601 gagtgaaggt aaacctgcaa acattgttaa ctcctgctaa attgttggcg ctaattattt
      661 catgctaccc ggcacatagc cagtagagtc aggactgatg aagacgttat ctcccgctgt
      721 gattacttta ccctggcgtc aggacgccgc tgaattttat ttctcccgct taagccacct
      781 gccgtgggcg atgcttttac actccggcta tgccgatcat ccgtatagcc gctttgatat
      841 tgtggtcgcc gatccgattt gcactttaac cactttcggt aaagaaaccg ttgttagtga
      901 aagcgaaaaa cgcacaacga ccactgatga cccgctacag gtgctccagc aggtgctgga
      961 tcgcgcagac attcgcccaa cgcataacga agatttgcca tttcagggcg gcgcactggg
     1021 gttgtttggc tacgatctgg gccgccgttt tgagtcactg ccagaaattg cggaacaaga
     1081 tatcgttctg ccggatatgg cagtgggtat ctacgattgg gcgctcattg tcgaccacca
     1141 gcgtcataca gtttctttgc tgagtcataa tgatgtcaat gcccgtcggg cctggctgga
     1201 aagccagcaa ttctcgccgc aggaagattt cacgctcact tccgactggc aatccaatat
     1261 gacccgcgag cagtacggcg aaaaatttcg ccaggtacag gaatatctgc acagcggtga
     1321 ttgctatcag gtgaatctcg cccagcgttt tcatgcgacc tattctggcg atgaatggca
     1381 ggcattcctt cagcttaatc aggccaaccg cgcgccattt agcgcttttt tacgtcttga
     1441 acagggtgca attttaagcc tttcgccaga gcggtttatt ctttgtgata atagtgaaat
     1501 ccagacccgc ccgattaaag gcacgctacc acgcctgccc gatcctcagg aagatagcaa
     1561 acaagcagaa aaactggcga actcagcgaa agatcgtgcc gaaaatctga tgattgtcga
     1621 tttaatgcgt aatgatatcg gtcgtgttgc cgtagccggt tcggtaaaag taccagagct
     1681 cttcgtggtg gaacccttcc ctgccgtgca tcatctggtc agcactataa cggcgcgact
     1741 accagaacag ttacacgcca gcgatctgct gcgcgcagct tttcctggtg gctcaataac
     1801 cggggctccg aaagtacggg ctatggaaat tatcgacgaa ctggaaccgc agcgacgtaa
     1861 tgcctggtgc ggcagcattg gctatttgag cttttgcggc aacatggata ccagcattac
     1921 tatccgcacg ctgactgcca ttaacggaca aatatactgc tctgcgggcg gtggaattgt
     1981 cgccgatagc caggaagaag cggaatatca ggaaactttt gataaagtta ataagatatt
     2041 acgccaactg gagaagtaag acgtggaata ccgtagcctg acgcttgatg attttttatc
     2101 tcgctttcaa cttttgcgcc cgcaaattaa ccgggaaacc ctaaatcatc gtcaggctgc
     2161 cgtgttaatc cccatcgtcc gtcgaccgca accggggttg ttgctgactc agcgttcaat
     2221 tcatctgcgt aaacacgctg gacaagtggc attccctgga ggtgcagtcg atgacacgga
     2281 cgcatcagtt atcgccgccg cgctgcgcga agctgaagaa gaggtcgcta taccgccttc
     2341 cgccgttgaa gttatcggcg tgctgccgcc cgtcgatagc gtaactggct atcaggtaac
     2401 cccagtggtc ggcattatcc cgcccgatct gccgtatcgc gccagtgaag atgaagtctc
     2461 ggcggtgttt gaaatgccgc tcgcccaggc attacatctg ggtcgttatc accctttaga
     2521 tatctaccgc cgtggcgatt cacatcgggt atggctgtcc tggtacgaac agtattttgt
     2581 atggggaatg accgcaggca taattcgtga gctggcgctg caaattggtg tgaaaccctg
     2641 actatactta tctttacatc tacaaaacac tacttgagac aatcatcgca atattagtta
     2701 aatcgcggtt tttgattagt ttaattcatg tgaatagtta agccagtcgc cgcgttccct
     2761 cttacactat gcgctgttat tagttcgtta ctggaagtcc agtcaccttg tcaggagtat
     2821 tatcgtgatt agtctattcg acatgtttaa ggtggggatt ggtccctcat cttcccatac
     2881 cgtagggcct atgaaggcgg gtaaacagtt cgtcgatgat ctggtcgaaa aaggcttact
     2941 ggatagcgtt actcgcgttg ccgtggacgt ttatggttca ctgtcgctga cgggtaaagg
     3001 ccaccacacc gatatcgcca ttattatggg tcttgcaggt aacgaacctg ccaccgtgga
     3061 tatcgacagt attcccggtt ttattcgcga cgtagaagag cgcgaacgtc tgctgctggc
     3121 acagggacgg catgaagtgg atttcccgcg cgacaacggg atgcgttttc ataacggcaa
     3181 cctgccgctg catgaaaacg gtatgcaaat ccacgcctat aacggcgatg aagtcgtcta
     3241 cagcaaaact tattattcca tcggcggcgg ttttatcgtc gatgaagaac actttggtca
     3301 ggatgctgcc aacgaagtga gcgtgccgta tccgctcaaa tctgccaccg aactgctcgc
     3361 gtactgtaat gaaaccggct attcgctgtc tggtctcgct atgcagaacg aactggcgct
     3421 gcacagcaag aaagagatcg acgagtattt cgcgcatgtc tggcaaacca tgcaggcatg
     3481 tatcgatcgc gggatgaaca ccgaaggtgt actgccaggc ccgctgcgcg tgccacgtcg
     3541 tgcgtctgcc ctgcgccgga tgttagtttc cagcgataaa ctgtctaacg atccgatgaa
     3601 tgtcattgac tgggtaaaca tgtttgcgct ggcagttaac gaagaaaacg ccgccggtgg
     3661 tcgtgtggta actgcgccaa ccaacggtgc ctgcggtatc gttccggcag tgctggctta
     3721 ctatgaccac tttattgaat cggtcagccc ggacatctat acccgttact ttatggcagc
     3781 gggcgcgatt ggtgcattgt ataaaatgaa cgcctctatt tccggtgcgg aagttggttg
     3841 ccagggcgaa gtgggtgttg cctgttcaat ggctgctgcg ggtcttgcag aactgctggg
     3901 cggtagcccg gaacaggttt gcgtggcggc ggaaattggc atggaacaca accttggttt
     3961 aacctgcgac ccggttgcag ggcaggttca ggtgccgtgc attgagcgta atgccattgc
     4021 ctctgtgaag gcgattaacg ccgcacggat ggctctgcgc cgcaccagtg caccgcgcgt
     4081 ctcgctggat aaggtcatcg aaacgatgta cgaaaccggt aaggacatga acgccaaata
     4141 ccgcgaaacc tcacgcggtg gtctggcaat caaagtccag tgtgactaat acttcttact
     4201 cgcccatctg caacggatgg gcgaatttat acccgctttc tcgtctgctg taatattccc
     4261 cactacactt ccactgttgc gtcaggcgtt tgtcgccata cgcttacagg gtggcccgca
     4321 tgcaaaaagc acaacggatc attaaaacct atcgccgtaa tcgaatgatt gtttgtacga
     4381 tttgcgcact cgttacgctc gcttcgaccc tgagcgtgcg atttatttca cagcgtaact
     4441 taaatcaaca acgggtagta caattcgcca atcacgctgt agaggaatta gataaagtac
     4501 tgcttcccct acaggcaggt agcgaagtct tgcttccgct gattggtctg ccctgctctg
     4561 tcgcccattt gccattacgt aaacaggcgg caaaactcca aactgtgcga tccattggcc
     4621 tggtgcaaga cggcacactt tattgctcca gcatttttgg ttatcgcaat gtgcccgtcg
     4681 tggacattct ggctgaactt cctgcaccgc aaccactttt acgcctgacg atcgaccgtg
     4741 ccctgattaa aggcagtccg gttttgattc aatggacgcc tgcagcgggc agtagcaatg
     4801 ctggggtcat ggagatgatt aacatcgact tactgacggc aatgctcctt gagccacaac
     4861 tgccgcaaat cagtagcgcc agcctgacgg tggacaaacg gcatttgctc tatggtaatg
     4921 ggctggtaga ttcccttccg caacctgaag acaatgaaaa ctatcaggtt tcttcgcaac
     4981 gctttccttt taccattaac gttaatggtc cgggggctac ggcgctggca tggcactatc
     5041 ttccaacaca attaccgctg gcggtgctgc taagtttact ggtgggctac attgcctggc
     5101 tggcgaccgc ttaccggatg agcttttccc gcgaaatcaa tctgggcctg gcgcaacatg
     5161 agttcgaatt gttctgtcag cctttgctta atgcgcgcag ccagcaatgt attggtgtag
     5221 agattttgct gcgctggaac aatccgcgtc agggctggat ttcaccggat gtgtttattc
     5281 ctatcgcgga agaacatcat ttaattgtgc cactgacccg ctatgtgatg gcagaaacca
     5341 ttcgtcagcg ccatgttttc ccgatgagta gtcagtttca tgttggcatt aacgtcgcac
     5401 ccagccattt tcgccgtggt gtgctgataa aagatctcaa tcagtactgg tttagcgctc
     5461 acccgattca gcaactgatc ctcgaaatca ccgaacgcga tgccttactg gatgttgatt
     5521 atcggattgc ccgcgagcta catcgtaaaa acgtcaaact ggcgattgat gacttcggca
     5581 ccggcaacag ttcattttcc tggcttgaaa cattacgtcc tgacgtgctg aaaattgata
     5641 agtcatttac cgcagctata ggttctgacg cggttaactc gacggtgacc gatatcatca
     5701 tcgcgctggg gcaaagactg aatattgaac tggtggcgga gggcgtggaa acgcaagaac
     5761 aggcgaagta tttgcgccgt catggcgtgc atattttgca agggtatttg tacgcacagc
     5821 cgatgccgct acgtgatttt cccaaatggc tggcgggcag ccaaccgccg cccgcccggc
     5881 ataatggaca tatcacgccc attatgccgt tacgttaacg cttactcatc ttcatcgtgc
     5941 gccggttgct ctttaacaat gcgaaccaga tcaacacgat aatcattggc ttcaatgatg
     6001 gtgatatgca gtggccctac atcaatcaca tcgcccacac ggggaatgtg accatttgcc
     6061 gagatcacca ggcccgcgac cgtcgcgata tcatcgtcat cggcaaggtg ctcaacatca
     6121 agcgcctgct gcaaggcatg caaatctgta ccgcctttta ccagccagcc gtcaccatcg
     6181 gtaatgattt ccggcgtttc gtcagcgtcc gggaattcac ccgcaatggc ttccagcaca
     6241 tccagcggcg tgaccaagcc ttgtaccaca ccaaactcgt tggtcacgat aacaaagctc
     6301 ccgcgagcgc gacgcagcac gcccagcagg ttgatcggat cgagggtttc cgggacgata
     6361 atcgccggag acgccgaagc aatcgccgcc acatcaacgc cctcttccag cgccaccagc
     6421 agttctttag cacgtacgat tccgatgatt tcatccagtt caccgcgaca taccgggaac
     6481 aggctgtgcg gtgaggagag cagctgctcg cggatttcat cgaccccgag attagcgtca
     6541 acccagctta tttcaccgcg cggcgtcatg atcccgcgca gagaacgcga cgccagcgtc
     6601 agtacgccgt taatcatgta acgttcttct tcggcaaatg caccttccgg gatcggcatc
     6661 ggcatcgggt tatcggcatc gtgctgaaca ttggcctgac gtttcccgcc catcaaacgc
     6721 aggatggcat cggcagtacg cgctcgcagc ggcaaagtcg actggtgacg aataaagttg
     6781 cgacgcgcaa tctggttaaa cacttcgatg atgatcgaga agccaatcgc ggcatacagg
     6841 taacctttcg gaatgtggaa accgaaacct tctgccacca gactcagacc aatcattaac
     6901 aggaagctca gacagagcac caccaccgtg gggtgctggt taacgaatcg cgtcagcggt
     6961 ttggatgcca gcaacataac cgccatcgca atcactaccg ccgccatcat caccggcaga
     7021 tggttaacca tccctactgc agtaattacc gcatccaacg agaagacggc gtcaaggatg
     7081 acaatctgtg tgacgccccc ccagaaactg gcgtagcctt taccgtggcc ggaatcatga
     7141 tcgcggtttt ccagccgttc atgcagttcg gttgttgctt tgaacagcaa gaatatcccc
     7201 ccgaacaaca taatcaggtc gcgtccggag aaggagaaat ccatgacggt aaatagcggt
     7261 ttggtcagcg tgaccatcca tgaaatcagc gacagcagcc ccagacgcat aatcagcgcc
     7321 agtgataacc ccagcaaacg cgctttatcg cgttgttttg gcggcagttt gtcagcaaga
     7381 atggcgatga agaccaggtt atcgataccc agcacaattt cgagaacaac aagcgtgagt
     7441 agccccgccc aaattgaggg gtccattaag aattccatga caagctcctg cttaaggaat
     7501 agctattcga cgccagaaat aatgcaggcg taacgacaaa atgcaaacga aaggtgcggc
     7561 atagagagcc agaaaggcag gcgttaaaag gcctgatgct gaaatgacgt cggtgacgat
     7621 ccatactgcg ggctactgcc ctatactcca tggttgttaa acgggagtta aacatatcag
     7681 agacgcctct gatttggcaa agatttacct tcctttgcaa acgaatgtga caaggatatt
     7741 ttacctttcg aaatttctgc taatcgaaag ttaaattacg gatcttcatc acataaaata
     7801 attttttcga tatctaaaat aaatcgcgaa acgcaggggt ttttggttgt agcccttatc
     7861 tgaatcgatt cgattgtgga cgacgattca aaaatacatc tggcacgttg aggtgttaac
     7921 gataataaag gaggtagcaa gtgaccattg ctattgttat aggcacacat ggttgggctg
     7981 cagagcagtt gcttaaaacg gcagaaatgc tgttaggcga gcaggaaaac gtcggctgga
     8041 tcgatttcgt tccaggtgaa aatgccgaaa cgctgattga aaagtacaac gctcagttgg
     8101 caaaactcga caccactaaa ggcgtgctgt ttctcgttga tacatgggga ggcagcccgt
     8161 tcaatgctgc cagccgcatt gtcgtcgaca aagagcatta tgaagtcatt gcaggcgtta
     8221 acattccaat gctcgtggaa acgttaatgg cccgtgatga tgacccaagc tttgatgaac
     8281 tggtggcact ggcagtagaa acaggccgtg aaggcgtgaa agctctgaaa gccaaaccgg
     8341 ttgaaaaagc cgcgccagca cccgctgccg cagcaccaaa agcggctcca actccggcaa
     8401 aaccaatggg gccaaacgac tacatggtta ttggccttgc gcgtatcgac gaccgtctga
     8461 ttcacggtca ggtcgccacc cgctggacca aagaaaccaa tgtctcccgt attattgttg
     8521 ttagtgatga agtggctgcg gataccgttc gtaagacact gctcacccag gttgcacctc
     8581 cgggcgtaac agcacacgta gttgatgttg ccaaaatgat tcgcgtctac aacaacccga
     8641 aatatgctgg cgaacgcgta atgctgttat ttaccaaccc aacagatgta gagcgtctcg
     8701 ttgaaggcgg cgtgaaaatc acctctgtta acgtcggtgg tatggcattc cgtcagggta
     8761 aaacccaggt gaataacgcg gtttcggttg atgaaaaaga tatcgaggcg ttcaagaaac
     8821 tgaatgcgcg cggtattgag ctggaagtcc gtaaggtttc caccgatccg aaactgaaaa
     8881 tgatggatct gatcagcaaa atcgataagt aacgtattgt gttgattatc actcagtttt
     8941 cacacttaag tcttacgtaa acaggagaag tacaatggag attaccactc ttcaaattgt
     9001 gctggtattt atcgtagcct gtatcgcagg tatgggatca atcctcgatg aatttcagtt
     9061 tcaccgtccg ctaatcgcgt gtaccctggt gggtatcgtt cttggggata tgaaaaccgg
     9121 tattattatc ggtggtacgc tggaaatgat cgcgctgggc tggatgaaca tcggtgctgc
     9181 agttgcgcct gacgccgctc tggcttctat catttctacc attctggtta tcgcaggtca
     9241 tcagagcatt ggtgcaggta tcgcactggc aatccctctg gccgctgcgg gccaggtact
     9301 gaccatcatc gttcgtacta ttaccgttgc tttccagcac gctgcggata aggctgctga
     9361 taacggcaac ctgacagcga tttcctggat ccacgtttct tctctgttcc tgcaagcaat
     9421 gcgtgtggct attccggccg tcatcgttgc gctgtctgtt ggtaccagcg aagtacagaa
     9481 catgctgaat gcgattccgg aagtggtgac caatggtctg aatatcgccg gtggcatgat
     9541 cgtggtggtt ggttatgcga tggttatcaa catgatgcgt gctggctacc tgatgccgtt
     9601 cttctacctc ggcttcgtaa ccgcagcatt caccaacttt aacctggttg ctctgggtgt
     9661 gattggtact gttatggcag tgctctacat ccaacttagc ccgaaataca accgcgtagc
     9721 cggtgcgcct gctcaggcag ctggtaacaa cgatctcgat aacgaactgg actaacaggt
     9781 gagcgaaatg gttgatacaa ctcaaactac caccgagaaa aaactcactc aaagtgatat
     9841 tcgtggcgtc ttcctgcgtt ctaacctctt ccagggttca tggaacttcg aacgtatgca
     9901 ggcactgggt ttctgcttct ctatggtacc ggcaattcgt cgcctctacc ctgagaacaa
     9961 cgaagctcgt aaacaagcta ttcgccgtca cctggagttc tttaacaccc agccgttcgt
    10021 ggctgcgccg attctcggcg taaccctggc gctggaagaa cagcgtgcta atggcgcaga
    10081 gatcgacgac ggtgctatca acggtatcaa agtcggtttg atggggccgc tggctggtgt
    10141 aggcgacccg atcttctggg gaaccgtacg tcctgtattt gcagcgctgg gtgctggtat
    10201 cgcgatgagc ggcagcctgt taggtccgct gctgttcttc atcctgttta acctggtgcg
    10261 tctggcaacc cgttactacg gcgtagcgta tggttactcc aaaggtatcg atatcgttaa
    10321 agatatgggt ggtggcttcc tgcaaaaact gacggaaggg gcgtctatcc tcggcctgtt
    10381 tgtcatgggg gcattggtta acaagtggac acatgtcaac atcccgctgg ttgtctctcg
    10441 cattactgac cagacgggca aagaacacgt tactactgtc cagactattc tggaccagtt
    10501 aatgccaggc ctggtaccac tgctgctgac ctttgcttgt atgtggctac tgcgcaaaaa
    10561 agttaacccg ctgtggatca tcgttggctt cttcgtcatc ggtatcgctg gttacgcttg
    10621 cggcctgctg ggactgtaag actgttgtac actaccgggg ccttttggcc ccgttttttt
    10681 atctggagga ttaatgacaa tcacggacct ggtactgatt cttttcatcg ccgcactcct
    10741 ggccttcgcg atctacgatc agttcatcat gccccgccgt aacggcccca ccctgctggc
    10801 aattcctttg ctccggcgtg gtcgcatcga tagcgttatc ttcgtcggat tgattgtcat
    10861 tcttatctat aacaacgtca cgaatcatgg tgcgttaata acgacatggt tattaagcgc
    10921 actggctctg atgggttttt atatattctg gatccgcgtt ccgaagatca tctttaaaca
    10981 aaaaggtttt ttcttcgcca atgtctggat tgaatatagc cgaatcaaag cgatgaactt
    11041 gtcggaagat ggcgtgttgg tgatgcaatt agaacagcgt cggctgttaa tccgcgttcg
    11101 aaatatcgac gatctggaaa aaatttataa gcttctcgtt tcaactcaat aagttatgaa
    11161 tttagccaaa gctatgttta gtgtattttt aataatcaga catagcttag gctatattac
    11221 ctcttccctt atttgttatt tattttaacg tttcattgat atataaatct aaatgtaaat
    11281 cgttatcaat aaagcaatga aataatatat tccaacagtt gttttatatt ctcaaaatat
    11341 gttaaggttg cgccctcatt tggggagtag ccgatttcca gattccggaa atgtacgtgt
    11401 caacatactc gttgcaaaac gtggcacgta cggactgaat actttcagtc aggcgagacc
    11461 atatgcacat caatcgctat gcctgcatga acgcgaaaac catcgcggct tgttgttcat
    11521 gtatgcatgt ttgctggggg cgatgatgtg ctttatggat accccggtca ggacattgtc
    11581 atgaatatca ctgctactgt tcttcttgcg tttggtatgt cgatggatgc atttgctgca
    11641 tcaatcggta aaggtgccac cctccataaa ccgaaatttt ctgaagcatt gcgaaccggc
    11701 cttatttttg gtgccgtcga aaccctgacg ccgctgatcg gctggggaat gggcatgtta
    11761 gccagccggt ttgtccttga atggaaccac tggattgcgt ttgtgctgct gatattcctc
    11821 ggcgggcgaa tgattattga gggttttcgt ggcgcagatg atgaagatga agagccgcgc
    11881 cgtcggcacg gtttctggct actggtaacc accgcgattg ccaccagcct ggatgccatg
    11941 gctgtgggtg ttggtcttgc tttcctgcag gtcaacatta tcgcgaccgc attggccatt
    12001 ggttgtgcaa ccttgattat gtcgacatta gggatgatgg ttggtcgctt tatcggctca
    12061 attattggga aaaaagcgga aattctcggc gggctggtgc tgatcggcat cggcgtccag
    12121 atcctctgga cgcacttcca cggttaataa gaacgctgcc agaggtgaat attaaagtcc
    12181 gtctggcagt cgaacacttc ttttgctgcc agtgtttgcc agacttctgg cttcgcacgc
    12241 caggcaaacg gcgtcatctg caataatgcg acggcttcat caccgcgaag acgcatcgga
    12301 taacacaact catcactctg ctgtaatgta aaaccttcca gttgttctgc atgaggtgca
    12361 tgaagatgta cttcattgta aatcagcccc ttcagctcca tcaaatgtcg cggtcccggc
    12421 gtggcagtaa tgacccagcc gccgggcttc actactcgtg ctaattcttc tgctttacac
    12481 ggcgcgtaaa tacgtattat ggcgtccata ctggtatcgg aaaacggcaa acggtggctg
    12541 gaagcgacac aaagagtgac ctgcggatag cgtttcgccg ccgcttttat cgctaccttc
    12601 gagacatcca ggccaaacgt ggtgatttcg ggcaacacat cggcaaatgc gtgtgtgtaa
    12661 tacccttcac cacagccaat atccagcacc gccgtggcct tttcatcaag ccgttcccgc
    12721 agttgggcga caattgcatc acgcagcggc tgataatgtc cggcatctaa gaatgcgcgg
    12781 cgtgcttgta tcatttccgc gctgtcgccc ggatcacgag accgtttatg ctgaacgggc
    12841 agcagattga catacccttc tttcgccata tcaaactgat gtcgctgggg acagatatag
    12901 ctgttttttt cacgcgaaag aggctgatgg caaagtggac aggaaaacga catgacaact
    12961 ccggcaggat actaaaggcc gcaagtgtaa cgcgaatcac gcggcgggca aatatccgaa
    13021 cgccaggagc gaagataaag catagtaaaa agcctcgcat tcgacgaggc tttatagcga
    13081 gattgaagcg tattcacact tcagatcagt ggattcgatc agatagctgt tacgttaaca
    13141 gctgccggac ctttctggcc gtcctgaatt tcgaactcaa cgttctgacc ttcagccaga
    13201 gttttgaagc cattaccctg gatagcggag aagtgtacga acacatcttt gctgccatca
    13261 gccggagtaa tgaagccaaa acctttagac tcgttgaacc acttaacctg acctttaatc
    13321 tttgccattt gaaaaattcc ttagattgtt ttcttcgccc gcaggcataa catagataaa
    13381 actgacacat tactgcatga ggcaccaata taaggctcgg cagagaagcg gtattcaacg
    13441 tcaacgtgtt tactcaggac ttctttactg aaaatgccac acataaacag aactgtacct
    13501 cgtttaaccc gaaatctgtt atcacataca acgttaatta tggcaagcca tttttaaaca
    13561 tgtctcgatc agacgcacaa atctcgatac gtcttccact ttttttgcac acttatgcaa
    13621 cggaatacgc gccgatatat cattgtgctt aaccttgcca gttcaggcag atacttaaac
    13681 tggcgtattt tctaacatag ttcaataagt tcagttgttc caggccagtg ggagaagtta
    13741 ttacatagtg cgtgcaatat cacatttttt gctatgcaat gaataaaaag ttatatcact
    13801 ttttctcata aaacagtcag ttaacggcta tcaattaccc taaagagaag tcaatcccca
    13861 aag
//
LOCUS       DAZFWP020000128        13677 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFWP020000128 DAZFWP020000000
VERSION     DAZFWP020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.128"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..863
                     /gene="hlyE"
                     /locus_tag="ACHK0Z_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /pseudo
     CDS             1..863
                     /gene="hlyE"
                     /locus_tag="ACHK0Z_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1070..1516)
                     /gene="ycgN"
                     /locus_tag="ACHK0Z_RS18455"
                     /old_locus_tag="pgaptmp_003691"
     CDS             complement(1070..1516)
                     /gene="ycgN"
                     /locus_tag="ACHK0Z_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU4503644.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1608..2267)
                     /gene="ycgM"
                     /locus_tag="ACHK0Z_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             complement(1608..2267)
                     /gene="ycgM"
                     /locus_tag="ACHK0Z_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4503645.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2339..2632)
                     /locus_tag="ACHK0Z_RS18465"
                     /old_locus_tag="pgaptmp_003693"
     CDS             complement(2339..2632)
                     /locus_tag="ACHK0Z_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU4503646.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2874..3275
                     /gene="pliG"
                     /locus_tag="ACHK0Z_RS18470"
                     /old_locus_tag="pgaptmp_003694"
     CDS             2874..3275
                     /gene="pliG"
                     /locus_tag="ACHK0Z_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU4503647.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3395..3763)
                     /gene="ycgJ"
                     /locus_tag="ACHK0Z_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     CDS             complement(3395..3763)
                     /gene="ycgJ"
                     /locus_tag="ACHK0Z_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU4503648.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4283..4978
                     /gene="minC"
                     /locus_tag="ACHK0Z_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     CDS             4283..4978
                     /gene="minC"
                     /locus_tag="ACHK0Z_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU4503649.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5002..5814
                     /gene="minD"
                     /locus_tag="ACHK0Z_RS18485"
                     /old_locus_tag="pgaptmp_003697"
     CDS             5002..5814
                     /gene="minD"
                     /locus_tag="ACHK0Z_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU4503650.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5818..6084
                     /gene="minE"
                     /locus_tag="ACHK0Z_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             5818..6084
                     /gene="minE"
                     /locus_tag="ACHK0Z_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU4503651.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6456..6800)
                     /locus_tag="ACHK0Z_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /pseudo
     CDS             complement(6456..6800)
                     /locus_tag="ACHK0Z_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6835..6954)
                     /locus_tag="ACHK0Z_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     CDS             complement(6835..6954)
                     /locus_tag="ACHK0Z_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4503652.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6915..7100)
                     /gene="ymgJ"
                     /locus_tag="ACHK0Z_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             complement(6915..7100)
                     /gene="ymgJ"
                     /locus_tag="ACHK0Z_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGU4503653.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7201..7374
                     /gene="ymgI"
                     /locus_tag="ACHK0Z_RS18510"
                     /old_locus_tag="pgaptmp_003702"
     CDS             7201..7374
                     /gene="ymgI"
                     /locus_tag="ACHK0Z_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGU4503654.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7376..7720
                     /gene="ymgG"
                     /locus_tag="ACHK0Z_RS18515"
                     /old_locus_tag="pgaptmp_003703"
     CDS             7376..7720
                     /gene="ymgG"
                     /locus_tag="ACHK0Z_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGU4503655.1"
                     /translation="MKKKILTFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7724..8059
                     /gene="ymgD"
                     /locus_tag="ACHK0Z_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             7724..8059
                     /gene="ymgD"
                     /locus_tag="ACHK0Z_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGU4503656.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPDLSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8116..10437)
                     /locus_tag="ACHK0Z_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             complement(8116..10437)
                     /locus_tag="ACHK0Z_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4503657.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10554..10763)
                     /locus_tag="ACHK0Z_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             complement(10554..10763)
                     /locus_tag="ACHK0Z_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503658.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11163..11381)
                     /gene="ymgF"
                     /locus_tag="ACHK0Z_RS18535"
                     /old_locus_tag="pgaptmp_003707"
     CDS             complement(11163..11381)
                     /gene="ymgF"
                     /locus_tag="ACHK0Z_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGU4503659.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(11513..13036)
                     /gene="pdeG"
                     /locus_tag="ACHK0Z_RS18540"
                     /old_locus_tag="pgaptmp_003708"
     CDS             complement(11513..13036)
                     /gene="pdeG"
                     /locus_tag="ACHK0Z_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4503660.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13368..13616)
                     /gene="ymgC"
                     /locus_tag="ACHK0Z_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             complement(13368..13616)
                     /gene="ymgC"
                     /locus_tag="ACHK0Z_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HGU4503661.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3899 a         3181 c         2951 g         3646 t
ORIGIN      
        1 atcgaaactg cagatggagc attagatctt tataataaat atctcgatca ggtcatcccc
       61 tggcagacct ttgatgaaac cataaaagag ttaagtcgct ttaaacagga gtattcacag
      121 gcagcctccg ttttagtcgg cgatattaaa accttactta tggatagcca ggataagtat
      181 tttgaagcaa cccaaacagt gtatgaatgg tgtggtgttg cgacgcaatt gctcgcagcg
      241 tatatttttc tatttgatga gtacaatgag aagaaagcat ccgcccagaa agacattctc
      301 attaaggtac tggatgacgg catcacgaag ctgaatgaag cgcaaaaatc cctgctggta
      361 agctcacaaa gtttcaacaa cgcttccggg aaactgctgg cgttagatag ccagttaacc
      421 aatgattttt cagaaaaaag cagcttttcc agtcacaggt agataaaatc aggaaggaag
      481 cgtatgccgg tgccgcagcc ggtgtcgtcg ccggtccatt tggattaatc atttcctatt
      541 ctattgctgc gggcgtagtt gaagggaaac tgattccaga attgaagaac aagttaaaat
      601 ctgtgcagaa tttctttacc accctgtcta acacggttaa acaagcgaat aaagatatcg
      661 atgccgccaa attgaaatta accaccgaaa tagccgccat cggggagata aaaacggaaa
      721 ctgaaacaac cagattctat gttgattatg atgatttaat gctttctttg ctaaaagaag
      781 cggcaaaaaa aatgattaac acctgtaatg agtatcagaa aagacacggt aaaaagacac
      841 tctttgaggt acctgaagtc tgataagcga ttattctctc catgtactca aggtataagg
      901 tttatcacat ttaaaaacac agacgttttt taaagattga gagttacctg ttgataataa
      961 ggtatcgggt aactctcatt atgacagagc gctttcaaaa acagggctac gaaatggcga
     1021 gcatccagac gtatattaag aaagccccca tcaacatcta ttaaatttat cactgcgccc
     1081 agtcaggttt atttaagata tgatcctgcc agtcaatcac ttctgattct ttcactgcga
     1141 tatgacgcac agagatacgt tcaccatgca ttgccgcttt cgaaccagta agtagcggat
     1201 gccacgcagg taaatcttta ccttccgcca gcaaacgata agcgcaggtc attggcagcc
     1261 attcgaatgt tggcagattt tcacgggtta atttaatgca gtcgggttca aactcgaaac
     1321 gacgttcgta gttccgacat tgacaagttt taatattgag ctggcgacag gcgacgttag
     1381 tgaagtagat ttcgtcggtg tcttcatcca tcagtttatg caggcaacac tgaccgcaac
     1441 catcacacaa cgactcccat tccgcatcgc tcatttcgtc cagggtttta ctttgccaga
     1501 aaggtacatc gctcatcagg tgttccgcca ttacgttaaa acgcacctta taaccagtct
     1561 ggcacagcga tgcaagtttt gccgccgctt tcaggcggca aaaagtatta caaaacgcga
     1621 gttgtcaaag aatgcccatc gaaagtgact gtcagctcat caccgctttg caacgggccg
     1681 acgccatcag gcgtgcctgt caacacaacg tcaccggcct tgagggtaaa aaacttgctc
     1741 atataagcga tcaacggaac gattttatgg atcatgtccg cagtcgtacc ttgctggcgt
     1801 tgttcgccgt ttacgctcag gcccagcgtt gtattttgtg gatcgccggt aaattccgcc
     1861 gcggggataa acccggaaag cggacaagag ttatcaaacg ctttagcctt ttcccacggc
     1921 tgcccggctt tcttcatttt tccctgaaca tcacgcaatg tcagatcaag cgccacgccg
     1981 taaccggcaa tggctttgcg gacatgctct tccgttgcct gacgcagcgt cgcgccaatc
     2041 aacaccgcca gttcgacttc atgatgaact gaaccgaaat cggatgggat cgccaatggc
     2101 tgccgcagat cgcacagtgc cgtttctggt ttaataaaca gcactggctc ttcgggcact
     2161 gcgctgccca tctctttaat atgtttggca tagttactgc caacacagac tactttactc
     2221 accggataat ccagcagcgc accttgccag ttgtgatgtt gatacataac gttcccctca
     2281 ccttatcgtg gatatgaaaa cccgcgccga caaagataat gccaccgccg gatatcggtt
     2341 atttgttagt gtcgtctgtt ttctgcccca tgacggaaag atgttgcttc agcaaatctt
     2401 cgggtggtgg cggtaactgc aaatagtaac cttgctcggt taatgccagt ttaacctttt
     2461 caatatcagc attgaccagt ttcttgcgcc catccagcgg cagaatcatc gctaactgag
     2521 gctgaccaaa acctttcatc agttcctcag gaacacgcga aaaatcgtct tttttttcga
     2581 catataaata ggtctggtca cgcttgctgc ttcgataaat cacacaaaac atacttttac
     2641 tctttaagat gccaggtttt ggcataagct aacatcaatg aaacccgcgc cacaagccag
     2701 ccagctgtag atttctgcgg tttgttgagt ccatgcccct ttataagtaa tccccccggc
     2761 aaaaaatgcc gcgccgggat tttaccgaac gcagaaaagc cactggaatt ctcccgtaag
     2821 ggattgttat attataacag ttcatcgtac tcattctgaa caggagacta ccaatgaaaa
     2881 tcaagagcat cagtaaggct gtattgctgc ttgcactgct aacctccacg agctttgctg
     2941 cgggtaagaa tgtcaatgtt gagttcagca aaggccatag cagcgctcag tactccggcg
     3001 aaataaaggg atacgattac gatacatata ccttctatgc caaaaaaggt cagaaagtgc
     3061 atgtgagtat ctctaatgaa ggcgctgata cttacctctt cgggccagga atcgatgatt
     3121 cagttgattt atccagatat tcaccagaac tcgatagtca cggacaatac tcactccctg
     3181 cttctggcaa atatgagctg cgagtgctgc aaacccgaaa cgacgcccgt aaaaacaaaa
     3241 ctaaaaaata taacgtcgat attcaaatta agtaaatgtc aaccgacggg gactctcccc
     3301 gtcataaacg ttaacgcccc actcctctct gtcgctcaag aggttaatga cgaatagcag
     3361 acaatgccga atagaacata atgtaaacaa ggcatcactc acgacacata aataacatct
     3421 ttgtggtgat ttgattgatt tttccactcc gcttaccatc cacaccaaaa taacgatcat
     3481 cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta aattcgctgg
     3541 gatcgtagcc ttgtaatgat tgtaagtttt cagcggcttt ttcgccgaga tatttccttg
     3601 ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa acaacgccgc
     3661 acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag aacatcatcc
     3721 ccacacctga caatccgata taaaaaatac gcatcatatt catagcacat cctgataatc
     3781 caaccgtaca acacttatgc tcatttcatc atcaatccgt aatcaaaacc gctgacctgg
     3841 acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta tgatagagca
     3901 attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgccg caaaaaatgg
     3961 aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg gatatttaaa
     4021 caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa taagaacaat
     4081 caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat gaatcagcgc
     4141 catttatcac agaatagact tttactctga ataaatggga gggtgacttg cctcaatata
     4201 atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg cgatgattaa
     4261 tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag gcagtagctt
     4321 cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc aggcgctgga
     4381 agacaaaatc gctcaggccc ccgcattttt aaaacatgcc cccgttgtac tcaacgtcag
     4441 tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg caaccggttt
     4501 gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa ttgaaaagat
     4561 ggggctgcct atcctgacgg aaggaaagga aaaagcgccg cgtccagctc ccgcaccaca
     4621 ggctccagcg caaaatacaa cgccggtcac aaaaacgcgt ttaatagata ccccggtgcg
     4681 ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc acgttagcgc
     4741 tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc gcggtcgtgc
     4801 gcttgcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc tgatggcgga
     4861 actggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag aattttatgg
     4921 caaagcggcg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt taaattgatc
     4981 cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg caaagggggt
     5041 gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa gggaaagaaa
     5101 actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat gggttgtgaa
     5161 cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct aaatcaggcg
     5221 ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca aacacgcgat
     5281 aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa agcgatggat
     5341 tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt aatggcactc
     5401 tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt acgcgactct
     5461 gaccgtattt taggcattct ggcgtcgaaa tcacgccgcg cagaaaatgg cgaagagcct
     5521 attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag aggtgacatg
     5581 ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt gatcccagag
     5641 gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga cattaacgcc
     5701 gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga acgtcctttc
     5761 cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg ataagttatg
     5821 gcattactcg atttctttct ctcgcggaag aaaaacacag ccaacattgc aaaagaacgg
     5881 ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta tctgccgcag
     5941 ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc tgagatggta
     6001 accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa cgtgacctta
     6061 ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt caaggcagag
     6121 tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc ggcgagtatt
     6181 cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag gctgttccca
     6241 cgcacagaac aaaatgttta tgcctggttg agtaaataac cctgttgtta gtgtggatat
     6301 actgggagat gataaccccg caatttcatt attcattgtt ctcccatagg atgaggtttc
     6361 ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat cctgtaaacg
     6421 aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt cacaccagcc
     6481 accgcgttcc acggggactc cacaccggca ccatggctat accccacccc gaaatgcccg
     6541 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
     6601 aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc gacaaattct
     6661 ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag acgcatgttg
     6721 taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga tttcgattcc
     6781 atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc aacatcagcc
     6841 cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat tgtgttcacc
     6901 gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag gcatttttta
     6961 cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata atactgtcgt
     7021 acgcttatct ttcgacaatg aggccgtcgg caaaataaaa tgactaatgt aatcgttgct
     7081 gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc agattatctt
     7141 acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata ttgtcaatca
     7201 atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat tcctatcatc
     7261 acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt taagcagatc
     7321 ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat ctaagttgaa
     7381 gaaaaaaata cttaccttcg ggcttatctc agcattgttc tgctccacgc cagcgatggc
     7441 ggatatgaat cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt tacttactgg
     7501 caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg gtgcagtaac
     7561 agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtaggtg ccgccgttgg
     7621 tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag gtgccgtgat
     7681 tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga tgaaaaaatt
     7741 tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg actacaatat
     7801 taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg aaatggcaaa
     7861 aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca gcgttgccag
     7921 ccgtgattta aaaattgagc aatctccgga tctgagcgcc aaagtggtag aaaaactgaa
     7981 tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg acaccatgcg
     8041 tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga cgcggttttt
     8101 tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg tgtcattgta
     8161 gccattatca cccagctgca cgcccacatt cccccagacg ctggccgctg gattcaggtt
     8221 gccatttacg ccaagtttaa tttcccccaa attactcgcc ccatcttgtt cgactttcac
     8281 accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt caacaaaagg
     8341 ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa aggtgcggaa
     8401 accaagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc cattggcttc
     8461 ggtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct ggaatatcca
     8521 cgtgtatttc gccgcttcca gaccaaagga tttattcagg gcaaagccgt aacccgcttc
     8581 cagcgagacg gttgcgcctt tcgcactata ggattcatag gctaattcat cccctttcac
     8641 cgagttttta aaccagctat attgcgccca ggcgtcaatg tatgcgcctt ttttactgat
     8701 gtcatcggca aaccaggtgg catacagccc tgcgcaatag cctctgacac tccctttcga
     8761 acgataatcc gacacgctgg aatgagttga gttgtagtcg cgggcataac cagccatcac
     8821 tcccaaacgc caactgtcac tgtcggtaaa accaccggtt aacagatcgg cccctaactg
     8881 cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg
     8941 cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta caggatcgat
     9001 gtagcgcatt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt tagcggctgc
     9061 caggttggcg acgtaacttc cagcttcgag gcgcaggacg ggggtaggcg caacagtagg
     9121 cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt tagtcagata
     9181 ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt aggcaccggc
     9241 gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc tgataatctc
     9301 tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa ctcgggttgt
     9361 tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat cacccaattc
     9421 agttctgaga tgcaatacgc cattattacc aacgtaattt tcagttattg ttaatcttgt
     9481 tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat caaccgtgag
     9541 gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa gcgacagata
     9601 ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg aattagttgc
     9661 atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg atgatacctc
     9721 actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt catttgctga
     9781 ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgaccattta tggaagactc
     9841 cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg tcaccgtgcc
     9901 gccattataa attttagcag tcgcggcgtc aggacccgtt gtataaattt ctccctggtt
     9961 aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat gcgtggtgat
    10021 atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac cagccttgac
    10081 gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga tctccccttt
    10141 tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg tagtactttc
    10201 ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg caagaacgcc
    10261 gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa aagagggggt
    10321 aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc cactcgtttg
    10381 acttagcaaa ccaattgagt aatcccccgt tatgacaatc gtgtttttac cctgcaaaat
    10441 aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgtgc ttagaggact
    10501 attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa tggttaactt
    10561 gctaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat aactagcgca
    10621 ttatcaatat cttttacttt tccatctttt attgccacag catgtttata ttcatcgtgc
    10681 tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc caaactaaac
    10741 ccggagagtt ttttcagttt catagattaa ccatccattc attggattaa caaacattcc
    10801 tggtcaaata acctatgcgt tcagattacc aaaacggagg gtaaagacaa aaacaaaagc
    10861 acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag tcggcattat
    10921 catttttcac cttattttca tgacattgat cactttaggg tgacgatttt gtttaacaaa
    10981 taactcttcg acatcaaaat tgttaatagt tgcgatgagg attatttcgg tgatatataa
    11041 aaacaaccgt gataacacca gggttgacaa ctatttgcgc aataatgcct ttccttcaat
    11101 gatccaggcg ttcactgcca gcaacccatt cagccgcata ctgacagtga gaaactgaaa
    11161 gattattgct gatccctgtg gttgacgagt ctttctctta atcgattaac cagattgatc
    11221 gccagccaaa caagggcatt aagcaaattt atcgtcacaa gcacttctcc cagcccggta
    11281 ctgggttttg caataaggat gaccaggagg gtgatcagca taaatgcaat ggaaaagatg
    11341 atatagagag tgaaataatc cagattatta ctattgttca tgctactaat ggcaatcctg
    11401 tcaaaacttt caaacaatta tagcaaacaa aataatgaac aatagtaaca agaggcaacg
    11461 agctaattag ctgtctataa ttacaccgca ctatggcctt actgatcaat tttcactcaa
    11521 ccacaacctt caccttcggt ttagaaagga taattttgac caggtcgatg tatgtaacag
    11581 gtttatagaa ataataaccc tgctgaaatg tgatattatt ttggttcaga tagtcaagtt
    11641 gttctttcgt ttcgacacct tcagcgacga tactgatcga aagtttacgc gccagatcca
    11701 atacacaatc cagaattcgg gttgattctg ggtctgcgtt tacgcggcca acgaaactgt
    11761 gatcaatttt gatataatca atatgcaggt catgaagata agaaagccct gagtagccag
    11821 taccgaaatc atccagcgcg atgacaaaac cattttcatg cagaatgttc aaccgctgaa
    11881 ccagactttc atcaacattc aatggctcac gctcggtgac ttccagaaca aggtttaaat
    11941 cgcggcgggt aaaactgtca cggaaattta aacactcgtc gacaaacgtc ggcgaaataa
    12001 tatgcgaagc gctaaaatta attccaatat gaaaaccttc cggcaattta ctcgcgatag
    12061 cgttcatctg tctggcaacc tgattcatca ggctttgcgt aagcggaacg attaatccgg
    12121 atttttcagc aagtggaata aatgccgcgg gtgatatata tccaccgtga ggttgtttcc
    12181 agcgggctaa cacctcaact ccccgcaatg tcccttcccg accatttacc acaggttggt
    12241 aaaaagggac gatctcccca ttatttattg cccgtcgcag gatctcttca ggcgttgcgc
    12301 ttttattgaa atatctatca agcaggaagg cagcggcgca ggcgattaac agaataaata
    12361 ttaaaattcc aaagccttta tcgataagtc tgtagaagct aaagagtggc ggcgggttaa
    12421 agataatagt gaaatgatat tttgtggagt gaaccttctc ggtataggga taatgtccat
    12481 taagcgtcat cacatcaccc gttggtccaa taatgtcatc cgccacgcgt aatacatacc
    12541 ttaccccttt caagggtaca ttaagcgccc cgcgaatatg ttgatcactt atcgtaacca
    12601 aaatgcgcgt atctgcaaat tggttctgat agagcaggat aggtaatcta ttaacggtgt
    12661 cgattgctgg agccaacagt aaattactgt ccgggaaaac aggaatacga gacaacagga
    12721 cccgattccc aggcagggat gtacaccaga ctattccctg tttgagaatg atgattgtgc
    12781 gcaggtgagg tttcagagct gcttcagtgc caagctgata ttgctcctcg aggttgcatt
    12841 ccttcccggc aatgttaacc gctgtttgag tcgcttgtga cgcttcttca agtatatgat
    12901 tgatattgtt ggcggcatat ctcgctcccg ccaggtactc tgcttttgcg gaataccaga
    12961 gctggatgtt caaaatagcg acaccggtaa taaacaagca tatcgcaacg aggatgggta
    13021 tgagtgtatt gcgcatttgc ttgtcccggc caaagggttt aatacatgaa aatgccattc
    13081 attcgctcgc gctggcatct acagagagca tggtagagag cataagaaat atccacgccg
    13141 aagataaaat tttctccatc ttgatctaac ccattaatta ttgagcataa tgtaggcagt
    13201 acaaaataag ttaggcggga tatcaggcgt caagaatgga gagcgagaac tctccattct
    13261 tgacacctga tattgcggac ataataagaa agcataacgc ctgaaatgct cactttgcat
    13321 cagcatggtg atacagctga tgtttattct aaaaccttac tcaagttcta agagagcacg
    13381 gattccctgt cattaataat gatatccgtt agtgtagtct tatgcatttc aatgtactgc
    13441 aatatttgca gatagagatc agaaagaatc acactggttt catcttttaa acgcaagcgt
    13501 aaagcctgat caataatatg actatcaaca tacccatgcg tcatgaacac ctccctctcg
    13561 agatttttaa acaaagcaca ttgaaaaata aatctctctg ggattgaatt attcatgtca
    13621 gcctcgtaga aatattaacc caataaaatt atgctgtgta tagtctaaag cgacaga
//
LOCUS       DAZFWP020000129        13513 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFWP020000129 DAZFWP020000000
VERSION     DAZFWP020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.129"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..815
                     /locus_tag="ACHK0Z_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             <1..815
                     /locus_tag="ACHK0Z_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4503662.1"
                     /translation="NALQSQIITDNHFLHHPKVDSELTRKYERARLDKENIYLLPLAR
                     GNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLSLFMKSPSYPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPVSQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGNDPIIGI
                     EAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHK0Z_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHK0Z_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU4503663.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1944..3404)
                     /locus_tag="ACHK0Z_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             complement(1944..3404)
                     /locus_tag="ACHK0Z_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU4503664.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHK0Z_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     CDS             complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHK0Z_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU4503665.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5009..6352
                     /gene="gntP"
                     /locus_tag="ACHK0Z_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             5009..6352
                     /gene="gntP"
                     /locus_tag="ACHK0Z_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU4503666.1"
                     /translation="MHVLNILWVIFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTITNIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6595..7497)
                     /gene="fimH"
                     /locus_tag="ACHK0Z_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             complement(6595..7497)
                     /gene="fimH"
                     /locus_tag="ACHK0Z_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4503667.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7517..8020)
                     /gene="fimG"
                     /locus_tag="ACHK0Z_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             complement(7517..8020)
                     /gene="fimG"
                     /locus_tag="ACHK0Z_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGU4503668.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8033..8563)
                     /gene="fimF"
                     /locus_tag="ACHK0Z_RS18585"
                     /old_locus_tag="pgaptmp_003717"
     CDS             complement(8033..8563)
                     /gene="fimF"
                     /locus_tag="ACHK0Z_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU4503669.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8573..11209)
                     /gene="fimD"
                     /locus_tag="ACHK0Z_RS18590"
                     /old_locus_tag="pgaptmp_003718"
     CDS             complement(8573..11209)
                     /gene="fimD"
                     /locus_tag="ACHK0Z_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU4503670.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMIQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11276..12001)
                     /gene="fimC"
                     /locus_tag="ACHK0Z_RS18595"
                     /old_locus_tag="pgaptmp_003719"
     CDS             complement(11276..12001)
                     /gene="fimC"
                     /locus_tag="ACHK0Z_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4503671.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12038..12577)
                     /locus_tag="ACHK0Z_RS18600"
                     /old_locus_tag="pgaptmp_003720"
     CDS             complement(12038..12577)
                     /locus_tag="ACHK0Z_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503672.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLAYQ"
     gene            complement(12642..13190)
                     /gene="fimA"
                     /locus_tag="ACHK0Z_RS18605"
                     /old_locus_tag="pgaptmp_003721"
     CDS             complement(12642..13190)
                     /gene="fimA"
                     /locus_tag="ACHK0Z_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4503673.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3283 a         3479 c         3287 g         3464 t
ORIGIN      
        1 gcaatgccct tcagagtcaa attattacag ataatcactt ccttcatcat ccaaaagtcg
       61 acagtgagtt aacacgtaaa tatgaacgtg cacgcctgga taaggaaaat atctatcttc
      121 tgcctcttgc tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
      181 aacttgatat tagtaaagag ccctggccat ttaattatgt gacagaaaca tgcagagaat
      241 ccgatggtat tacaacaaca ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt
      301 tagacgaaat atatggtggt atatgtaaaa aggcgcatgc tgccacagag ttagcagaag
      361 gattacggct aagtttattt atgaagagcc cttcctatcc tgttgaggac tatacagttc
      421 atgaaataac attaggccct ggttgcaatg ttccaggtta cgccggaacc acgataggat
      481 atatttcaac acttccagtt tctcaggcaa aaagatggac caatgagcaa ccaaggattg
      541 atatctatat tgatcagata atgactgtct caggggtggc aaactcttcc ggattcgccc
      601 ttgccgcatt acttaatgct aatattgagt tgggtaatga cccaataatt ggtatagaag
      661 cctatcccgg taccgcggag atccatgcaa aaatgggata taaagttatc ccgggtgatg
      721 aaaatgcacc actcaaacgt atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      781 taaaaaatgg tgaatggaat tatattggta aatagccact gagaagaatg agtactaaca
      841 ttcaggttat acagatagct aaacaatacc ctgcaattta caaagtaata ctcattgcga
      901 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
      961 cgtcgacttt atccagcggc catgaatcaa acagatagcc atcaaaataa atatcgtcaa
     1021 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
     1081 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     1141 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attggattgt
     1201 tttcccgcca ctgccaggac tgacggaaca gctccaccag catgctgtta tgcgttgctt
     1261 cggcaatagc gagatggaac tgcatgtcac cgctttcgct gctgcccggc gcactggaag
     1321 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     1381 cctgcaaagc ggcaaactcg gcgatgttgc tctccaataa ctgccgcgcc tgtaacagct
     1441 caaaaggacc ggcatcgttg cagacgttgg catccggact gtctgtgttc tggctgcctg
     1501 agttatcaag aacatagata ccggcacccc ggcgtacttc caccagccct ttgatctcca
     1561 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
     1621 gctccggcgg cagccgttcg ccaggattgt acggcgtctt tatgatcaga tcgcggatca
     1681 tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc
     1741 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     1801 aacgggcaat tttacggccc ccgttaatct caaactatac cattagcact ggaagttggc
     1861 acgtataggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     1921 gagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     1981 cacagctgct gatatgccgc cgtcactgcg ccaacaaagt cggcattctg cggcagatca
     2041 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     2101 gcgttgacct tctggaactc cgccagcatc gggtcgacca cgtcaatggc attaccctgc
     2161 tcatccacgc cctgggtgta acgcatccag ccagccacgc ccagcgccag gtgacgccag
     2221 ctgccgccgt tttgcaggtg cagacgcacc gggtccagca gacgctgcgg taacttctgg
     2281 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     2341 atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag cgttggcgct
     2401 tgttcctgca tcatcaagga gaaggccgct ttgcgataat ccgggttagt catggtgtcg
     2461 gcaatggttt catagccgcc gaggtaaccg aggtatgcca ggaaagagtg gctgccgttc
     2521 agcatacgca gcttcatcat ttcgaacggc acaacgtctg caacgaactg tgcgcccact
     2581 ttatcccagt ccgggcgacc gttaacgaag ttatcttcaa tcacccactg acggaacggt
     2641 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgcaaggtt
     2701 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttttct
     2761 tcaatccatg ccgccagctg cgggtcacgc gcctgagcca gtcccagtac cgcgaccttc
     2821 gccacatggc cgttttcacg cacgttatcg caggacatca ccgtaaacgc tttcaaccct
     2881 ttttcacgac gcagccgcag ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     2941 gggttttcca gatcgtgctt gatcagtggg ttattgagat ccagctgacc gcttgccgca
     3001 tcagcgcagt agcctttttc cgtgactgtc agagagacaa tcgccgtctg cggacgcgcc
     3061 atcgcattga gaataccttc gcagccgtcg atttccggat gcagcgcttc tttcatcgaa
     3121 ccgataattt tcagctcggt gctctctgcg cctttttccg ctacggtgta cagcagttgc
     3181 tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag
     3241 atgccccagt cgctgtcggt gctttccagc agatgatggg tatacagcgc ctggtgcgcg
     3301 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     3361 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     3421 cgtcttcaaa gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc catgccggtg
     3481 gatattaacg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     3541 ggcctttcag acgaccaatt gcggagtaac ctgggttggt tttcttcttc aggtcgtcca
     3601 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     3661 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     3721 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     3781 tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg tcagcacgca
     3841 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     3901 tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg
     3961 gatcgtccgg gtgaacagcc atacggacgc caacttcttc ggcaaccgga ataatcgctt
     4021 tcaggaagac agcaaagttt tcgcgcagtt tggctttgtc gatatctttg tacagttcca
     4081 ggtgcttacg gaactggtcg agggtgtacc cttcttccgc acctggcaga ccagcgatga
     4141 tgttacgggt cagacgcgct ttgtcttcat cgctcatggt ggcgaagcga acagcagcct
     4201 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgtttcagg atatgcattt
     4261 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     4321 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     4381 tgccgcactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     4441 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttctacgaca gaccacacca
     4501 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     4561 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     4621 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     4681 tttcacatag tgggttccac agcaaagaga cgcggaaccc tggagttgtt taggtatccg
     4741 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     4801 cttttggtaa gacatgtcaa cccgaattct gaaattggtt aaccacatca caagaatttc
     4861 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4921 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     4981 acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattcttt gggtaatatt
     5041 cggcattggc ctgatgctgg tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     5101 gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt cgctgctgca
     5161 caccatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg tggtgttcgg
     5221 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cacatacgct
     5281 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gcctgatttt
     5341 cggtctggcg atgttttatg aagtggcctt tatcatgtta gcgccgctgg ttattgttat
     5401 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     5461 taccgcacat tcattgttcc caccgcagcc gggtccggtg gcgctagtga acgcttatgg
     5521 cgcggatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
     5581 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     5641 gaaagcagat caaccggtag atatgaataa tctgccctct ttcggcgttt cgattctggt
     5701 gccgctgatc ccagcgatca ttatgatctc caccaccatc accaatatct ggctggtaaa
     5761 agatacccct gcgtgggaag tggttaattt tattggttcc tcgccgattg cgatgtttat
     5821 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
     5881 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
     5941 gggtggcgtg ttgaagcaga ccatcatcga caccggcatt ggcgacacca tcggcatgtt
     6001 gatgtcccac ggcaatatct cgccctacat catggcatgg ctgatcaccg tactgattcg
     6061 tctggcgacg ggtcagggtg tggtttcagc gatgaccgcc gccgggatta tcagtgctgc
     6121 aatccttgat ccagcaactg gtcagctggt tggcgtgaat ccggcgctgc tggtactggc
     6181 gacggctgcg ggttccaaca ccctcaccca cattaatgat gcctcattct ggctgttcaa
     6241 aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtctgc tggagctggt
     6301 caactccgtg gttgggctga ttattgtgtt gattattagc atggtagcgt aaaaggacac
     6361 cagagccagc caatggcagg ctcagactga tgacaaagtc taaaaatgta cccggacagt
     6421 cccctcgccc ctccggggag agggttaggg tgaggggaac agaccagcaa agtgcgcttt
     6481 gtttatgcca gatgcgacgc tgacgcgtct tatctggcct acaaagggct aacgtgcagg
     6541 tttttagctt caggtaatat tgcgtaccag cattagcaat gtcctgtgat ttctttattg
     6601 ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct gccctccggt
     6661 acgtgcataa tttgccgtta atcccagact caccgccgaa gtccctactg ctcctaaaga
     6721 taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg
     6781 tgcaggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg cggttgtgcc
     6841 ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag gaattggcac
     6901 tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat cgcagccgcc
     6961 agtaggcacc accacatcat tattggcgta aatattccac acaaactgga aatcatcgct
     7021 gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag ctttaatcgc
     7081 caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg gcttatccgt
     7141 tctcgaatta taaacaacgc gcggcgtttc gctggtggta ggaaatggat agctactgcc
     7201 actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg agcctcgttg
     7261 cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga
     7321 aagatccacg accaggtttt gccccacatt cacggcgggc gcaaggttta cataaacatt
     7381 ggcgctgcca ccgccaatag ggatagcggt accattggcg gttttacagg cgaatgacca
     7441 ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc gtttcattac
     7501 aatcatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc tgaatggttc
     7561 cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag tgcgctgatt
     7621 gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg ttgccactgt
     7681 catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa tatccggtgc
     7741 tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga caattagtca
     7801 actcaagcgc aacatcatgc caggccgatg ccgccccggc agacataaga ctgaaagaat
     7861 aaagatcgcc gagatcaacc gtggcattgg tggtggaaac tgtgcacggt ttggcgacga
     7921 ccttaccgtt taccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt gcgagcgcca
     7981 atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc agttactgat
     8041 attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc acctgtgtcg
     8101 ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg gtcgtccacg
     8161 aaatcgcgga cgatggagca ttaaggggta tttgattttg ctgctcattc agaagctgta
     8221 ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcagattg gcattgtggc
     8281 tatctgcaac gccggtaaac ccaactttta cggcagaaac ggcattacca cagggtgaca
     8341 gcaaaatacg aaatggaaca acaggagtcg tcgcgccaat gttgttaaat tgcttcgccg
     8401 cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta cagccgttat
     8461 ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaagcgtga ctcaccgcca
     8521 gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc ccttaacgac
     8581 attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt tgataattgg
     8641 cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc gctaaaggca
     8701 ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg ctactctctg
     8761 atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc atgagcagtt
     8821 ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg ggaacaacgt
     8881 tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc agcgccactc
     8941 tgttttccct atattcagtg gcataaggca gcacggcata accgcgccag tcggtacgca
     9001 cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca agcaccaccg
     9061 tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccaatacc ccaccgctga
     9121 ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg gcattgccgt
     9181 aaccaccgcg ataattcagc gtggcgtagc ctgtgcttcc gctattacca tcgcctcccc
     9241 cggcatagcc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac gtaccgtata
     9301 caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag ctggcactgg
     9361 catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga atattgacgt
     9421 taagcgctaa catctgatca cgtccttttt gccaggcgtt tttcgtcagg ctatagctga
     9481 gcgtccagtt gatatcttcg aatgcagtat ttaatccagc ctggaattgc tcatcgacat
     9541 tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat gtgcgcccga
     9601 gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg ttgtaatagt
     9661 cggtgaattt cggcttaacc tgaataactc cgtcctgtgt ttcgatgttg tagccattca
     9721 ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa cggtaaccca
     9781 ctaactgaat attcgtgcct gactcattga gcgatttgtt atagagaaaa cgcaccgatt
     9841 gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata tccacagaca
     9901 gagcgcccag tgcccccatg tttttcccga taccgaaatt aaaagcacga taacgatccg
     9961 ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt aatgtactct
    10021 ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg gtattctcct gccgtaatgg
    10081 aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt acggtaaaaa
    10141 tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta ttacctgcgg
    10201 catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa atgtcatacc
    10261 cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc accggggcaa
    10321 atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg ccgcgaaagt
    10381 taataccatc gaaaatatcg ccctgagtat aaccatcacc cagcgtcagc cgggaacgta
    10441 acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt ttgctacctg
    10501 atgatctgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc cacgcaccaa
    10561 tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca atccgattct
    10621 gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg ggatcccata
    10681 actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg atcgtcaggt
    10741 tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc ctggatcatt gtggttaatg
    10801 gcacgcaggc atcatccgcc agcagattca taccggagac agaagccgta ttcagcccca
    10861 tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg tcgcccgtat
    10921 taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg acacgatacg
    10981 tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca gcctggggat
    11041 catccgctaa aaaacgcggg ttaaaataga gttcggcaga tgacaaaggt gcctgtgcgg
    11101 caaaagcaca ggcaacaaag agccggacaa aaaaaccagc caaacgatgc ttacgaatat
    11161 gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt attcctccgg
    11221 tttaaagtca cccggcagtc aattcttttt attcaggcga aaaattcccc ctgcgttatt
    11281 ccattacgcc cgtcattttg ggggtaagtg caccataatc atttattgtt cggtaagtaa
    11341 tattgcttcc cgcatcagaa ggcaatttaa ccgtgctttc gcccattgga ggcaccaagg
    11401 cattttcaag aacccgggtt ccggcattca actctgttac cgtcaggtaa tagggtgtcg
    11461 ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct gcggcctgat
    11521 cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg ataattgcga
    11581 gctgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct ttaacattca
    11641 tccagaataa actttcccga tcctgtggca attggttatt tgttgcatca agaatacgca
    11701 aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata aaacgaccat
    11761 cctttacacc atcggcattt tccacccatg attgaattaa ataggtacta ttttcatcat
    11821 tatttgtcac ggcaagttgc acttgttttt gcccggccgg ataaattacg cgagtcgcac
    11881 ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccataaac atcaggatac
    11941 ctgccagcaa gcagaatgtt atttcctgcg atttccttac attgacgttt ttattactca
    12001 ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggct aaagagaacc
    12061 aggcctgggc attggcgatg ccgccagtaa cccgacgccc ggtagcacga tatttggcga
    12121 tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttgctgga ggacgattaa
    12181 tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg gctatccctg
    12241 gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg tgaaacgcca
    12301 cccccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca aaaggcaccg
    12361 gtgcgctatc ttcccctgcc gcatgaaacc ggttactgct gatttgcccc atattgaccg
    12421 tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg ccctgaaatt
    12481 gcatatttcc gccaggcagc gtggtattcc atttatttcc ggccagagca aacattggca
    12541 gcaacgacgc taacagaaat aatcttttcc ttttcatcgt tatttttatc gcacaagggt
    12601 gggcatccct gcccgtaatg acgtccttga acctgggtag gttattgata ctgaaccttg
    12661 aaggttgcat ccgcattagc tgcacccggg gttgcctcgc cgattgcata ataacgcgcc
    12721 tggaacggaa tggtgttggt accgttattc agggttgttt gtgcactaaa tgtcgcaccg
    12781 tccagcgtca gagcattgcc tgttctgtcc aggatctgca cgccaacgtt tgttgcacta
    12841 cctgcagcag aactctgcag agccagtaca tcagtacgcg tagcatcaat tgccgtacct
    12901 aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc aatcattcag ctgaatgtta
    12961 aaaccaacgg cagagctggt tgctccagcc tgcttcaggc tagcggtacg aacctgtccc
    13021 aactgaacgg tttgatcaac agagcctgca tcaactgcgc aagcggcgtt aacaacttcc
    13081 cctttaaagt gaacggtccc accatttacc gtcgtagtat cggccagagc cgctgcggaa
    13141 ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat gctgctttcc
    13201 tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt gttttgtcaa
    13261 cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt tggggccatt
    13321 ttgactcata gaggaaagca tcgcgcacaa actttttcag tttatttgtt ggcttaatat
    13381 tctattgtta tctttattta tagattttta tattgcatga ggtggttttt ggagagaaga
    13441 atgaggaaga tgcgtcgagc cacagaaacg ttagctttac atatagcgga ggtgatgtga
    13501 aattaattta caa
//
LOCUS       DAZFWP020000130        13498 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFWP020000130 DAZFWP020000000
VERSION     DAZFWP020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.130"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHK0Z_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHK0Z_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4503674.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHK0Z_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             516..1217
                     /locus_tag="ACHK0Z_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU4503675.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHK0Z_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHK0Z_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU4503676.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHK0Z_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     CDS             complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHK0Z_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU4503677.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2954..4285)
                     /locus_tag="ACHK0Z_RS18630"
                     /old_locus_tag="pgaptmp_003726"
     CDS             complement(2954..4285)
                     /locus_tag="ACHK0Z_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4503678.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4560..5924)
                     /gene="tdcG"
                     /locus_tag="ACHK0Z_RS18635"
                     /old_locus_tag="pgaptmp_003727"
     CDS             complement(4560..5924)
                     /gene="tdcG"
                     /locus_tag="ACHK0Z_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4503679.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5996..6385)
                     /locus_tag="ACHK0Z_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             complement(5996..6385)
                     /locus_tag="ACHK0Z_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU4503680.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6399..8693)
                     /gene="tdcE"
                     /locus_tag="ACHK0Z_RS18645"
                     /old_locus_tag="pgaptmp_003729"
     CDS             complement(6399..8693)
                     /gene="tdcE"
                     /locus_tag="ACHK0Z_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU4503681.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8727..9935)
                     /gene="tdcD"
                     /locus_tag="ACHK0Z_RS18650"
                     /old_locus_tag="pgaptmp_003730"
     CDS             complement(8727..9935)
                     /gene="tdcD"
                     /locus_tag="ACHK0Z_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU4503682.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9961..11292)
                     /gene="tdcC"
                     /locus_tag="ACHK0Z_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             complement(9961..11292)
                     /gene="tdcC"
                     /locus_tag="ACHK0Z_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU4503683.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11314..12303)
                     /gene="tdcB"
                     /locus_tag="ACHK0Z_RS18660"
                     /old_locus_tag="pgaptmp_003732"
     CDS             complement(11314..12303)
                     /gene="tdcB"
                     /locus_tag="ACHK0Z_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU4503684.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12402..13340)
                     /gene="tdcA"
                     /locus_tag="ACHK0Z_RS18665"
                     /old_locus_tag="pgaptmp_003733"
     CDS             complement(12402..13340)
                     /gene="tdcA"
                     /locus_tag="ACHK0Z_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU4503685.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESGFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3535 a         3628 c         3159 g         3176 t
ORIGIN      
        1 cgcccccgcc agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccagac
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta
      961 cgatgggaag cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc
     1441 ggcctacaaa atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc
     1501 cagcatcgca tccggcatca ggtacaaaac acaacaacat caacaagttg agcaccccgt
     1561 aggccggata aggtgcgcca gcatcgcatc cggcacaata ctcaaaacct ggcgtttatc
     1621 tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg
     1681 ctaacgcaca caggttggca atcgactgct caacatcatg cgccacgatc ccttcattgc
     1741 cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg
     1801 tcgaaacctt catcgcgcag ctgttcgacg caccatcgca aatcatgccg ctgacatcgc
     1861 cgatcatact gctgatcgcc atcgagatgg tttcataacg cccatccacc agccatgcca
     1921 tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta
     1981 actggttatg gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagccgtt
     2041 catcatccgc tccgaagtgt tctgctacca ccaccacagg cattgttgcg gtgatcccct
     2101 ggttacccga gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg
     2161 atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct
     2221 cgcactgttt ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct
     2281 gcgataacgc acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaacggga
     2341 cttcattgac gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggag
     2401 actcttgctc gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct
     2461 caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt
     2521 tccagacttt ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacggaga
     2581 ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca
     2641 gcacttccag cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca
     2701 gccccaccat tcccgtgccg ggaacggtga cgcccagacc gttcttcatc agatttggcg
     2761 aaacccaggc ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg
     2821 ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg
     2881 cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa
     2941 aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac aaaccagtga
     3001 cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca
     3061 gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc
     3121 tcaacaccgg tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga
     3181 tgccgcgctg aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt
     3241 tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca
     3301 tgacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc
     3361 taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcataggct tttaccgctt
     3421 cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa
     3481 tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt
     3541 cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca
     3601 gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac
     3661 cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg accaccagca
     3721 gcttggtcag caccatgccg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga
     3781 tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt
     3841 ccgtcacgcc gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga
     3901 cgaacatcca aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt
     3961 tacctaaata accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg
     4021 tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca
     4081 aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa
     4141 tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat ttgatcgctt
     4201 ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga
     4261 cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt
     4321 tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa
     4381 taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt tgaaataacg
     4441 gagatggata agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg
     4501 gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt
     4561 cagccgcaga ccactttaat ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc
     4621 atatctttgc cggtttcata catcgtctcg atcactttat cgagtgaaac acgcggtgcc
     4681 gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt tcacggcatt aatggcatta
     4741 cgttcaatgc acgggatttg tacctgtccg gcaaccggat cgcaggtcag cccaaggtta
     4801 tgctccatcg cgatttccgc cgcattgcat acctgcgccg gactgccgcc cagtagttca
     4861 gttaaccctg ccgccgccat tgaacaggcc acgccaatct ccccctgaca gccgacctcc
     4921 gcgccagaga tggaggcgtt cattttatac agcgcgccaa tagcccccgc tgccagaaaa
     4981 tagcgggcaa ttgaccgctc gtttaccgga cgacggaact tatcgtaata agccagcact
     5041 gccggaataa tgccgcacgc accgttagtc ggtgccgtta ccacgcgccc gccagctgcg
     5101 ttttcttcac taaccgccag cgcgtacatg ttgatccagt cgatgacatt catcggatcg
     5161 ttagagatgt tatcgctgga aaccagctga cgacgcagcg ctacggcacg acgcggcaca
     5221 ttgagcggac caggcagcac gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc
     5281 acttgccaga tacgggcaaa ccctgcgtca atttccgctt tgctgcgcag cgccagctcg
     5341 ttgtgcatca tcaggccaga tatagacagg ccgttgtaat cacacatttt cagcagttca
     5401 cctgctgagt ggaaatcgta aggtacggac gtttcgacat cgtgcgacag gccgaagtgt
     5461 tcttcttcga caataaaccc gccgccgaca gagtaatagg ttttacttaa tagcgcttcc
     5521 tgtcccttcc aggcagtgat ccgcattccg ttctcatggc gaggcaacat ttcgggatgg
     5581 aagataatgt tctttgctac aggaaaatca acaatatgcg caccagatgc cactggcagc
     5641 cgcccgctgc gcgttactaa ctctataaat gcagggatct catcaatgac aacatcctgc
     5701 ggactgtttc ctgccagtcc catgataatg gcgacatccg tggcatggcc tttgcccgtc
     5761 agtgacaacg acccatacag atcgaccaca atatggctcg tcgcggttaa taagccgcta
     5821 ctttccagcc gatcaataaa actttttccg gcattcattg gccccacggt atgcgaactg
     5881 gagggaccaa tcccaatttt gaaaatatcg aatgcactaa tcatatccac accctcggat
     5941 tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt cccgctcacg aggctttacg
     6001 cactacgtac tgcgatagct tcaatttcca gcttcacatc tttcggcaaa cgcgcaacct
     6061 gcacacagct ccgggtcggg taggtcgcct gatgctcatc gaagaactgc ttatagactt
     6121 cattgatggt ggcaaaatca ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc
     6181 ccacgctcag cccggcagca accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct
     6241 gatcttgcac atcagccggg atctcaccgg tctgcgggca aaccggaatt tgcccggagg
     6301 tgaagaccat gctgcctaaa tcaacgccct gaacataagg gccgattgcg cctggggcac
     6361 gttgcgtttc gataatcttt ttcatacatc ctccggcgtc agagcgcctg ggtaaaagta
     6421 cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca
     6481 gagacacgga ttgtcaggtt aggatatttt tccgggtgct cgatggcatc cagcagcatt
     6541 tcccgattca ttacgttgac gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg
     6601 aaatacccat ccagcaggcc gacaaggttg gttttacgta ctggatcttc tttgcccagc
     6661 gccgcaggaa cgattgagaa ggtgtacgag atcccatctt tggcgtaggt gaacggcagt
     6721 ttcgccaccg acgtcaatga ggccacggca cctttgcggt cacgaccatg catcgggtta
     6781 gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg gcgtattacc ggttttctgg
     6841 ccgtacacca cgttagaagt gatagtcaga atcgactggg tagggacggc gttgcgatag
     6901 gttggcagcg ctttaatttt cttcataaag cgttcaacca ggtcgcaggc aatgctgtct
     6961 acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt cgatttcaaa gtccaccgcc
     7021 aggccgtttt cgtcacggat tggtttcacg cgggcatatt tgatggcaga cagggagtcc
     7081 gtcgccaccg acaggcccgc gatgccgcat gccatagtgc gatagacatc acgatcgtgc
     7141 agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc
     7201 gcgctgatgt actgcaccgc cagccagtcc atgaagtgat cgaggctgtc catcactttg
     7261 tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg gcccgacctg aatcttcagc
     7321 ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt tcgccaggtt agcgcgtgca
     7381 ccaaagaact gcatttgctt accaatcacc attgggctga cgcagcaggc aatcgcgtaa
     7441 tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt catactgcaa ggaagaggtg
     7501 acgatcgaca cctgcgcggc atattttttg aaggcaatcg gtaattcttc cgaccaaaga
     7561 atggtcaggt taggttccgg tgccggcccc atagtgtgca gggtgtgcaa atagcggaag
     7621 gagtttttgg tcaccagcgt acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc
     7681 cagattgggt cgccggagaa cagcgaatca aattccggtg tacgcaggaa gcgtaccata
     7741 cggatcttca tgatgaagtg atcgatcagt tcctgtgcct gctgctcatt gagtacgcca
     7801 gctttaaagt cgcgctcaat gtagatatcg aggaacgatg ccgtgcggcc cagcgacatc
     7861 gcgccgccat tttgcgattt cactgccgcc agataagcga agtagagcca ctgcaccgct
     7921 tcctgcgcat tctgcgccgg gcgagagata tcaaagccat atttcgccgc catttcctga
     7981 atctgcaaca gcgcatgacg atgctctgcc agctcctcac gcagacggat ggtggcttcc
     8041 agatcctcgc ctttttccag acgagactgg agatcggcaa attgcagttc gcgttcacgt
     8101 accagataac tgatgccata cagcgctacg cggcgatagt caccgataat gcgcccacgg
     8161 ccatagccat ctggtaaacc ggtcagcacg ccagatttac ggcagcgcag catatccggt
     8221 gagtaaacat caaatacgcc ctggttatgg gttttacgca gatcggtaaa cagatattca
     8281 aattcactgt ccatttcacg gccataggcg tggaatgaac ttttaatcat attaatgcca
     8341 ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt gcaggccaac aattttttcc
     8401 agcggctggt taatatatcc cgcatcatga gcggtaattg tggtggcaat attggtatcg
     8461 aaatcaaccg gcgcgtgggt tgcattttcg atacggatgc cttccattac tttttcccac
     8521 aattccgtgg tggcaggcgt cgcttcggcg aggaaagatt catcgccttc atacggtgta
     8581 tagttatgtt gaataaaatc gcggacatta atttcgtttt tccagtccgt acctttaaag
     8641 ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa tatctacctt catgaaaaat
     8701 aatctctcta caatacttca actaaattat gcaaattctg cgggcgcgtt aactttgcct
     8761 aaatgaatgg catccaaagc aatcattttt tcttcgttag tcggaataac ggcacaaatg
     8821 acacgcgcat tttcactgga aacaattcgc tcaccacagg agttagagcg attattcatt
     8881 tctgtatcaa tctctacgcc taatacagcc aaatgttcca tgaccagacg acgaattaag
     8941 cttgaattct ctcctattcc gccggtgaat ataattccat ccaggcgacg taatgaagct
     9001 gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg
     9061 cgttcgtgac cttcatgcca ggctttttcc agaacacgta aatccgaaga aagaccggaa
     9121 atacctaata atcccgactc tttattcact acgcgttcca ggtcacccag gctctggttg
     9181 gtttggctgg cgacccagga catcgcacca aagtcgacat cgccactgcg ggtacccatc
     9241 atcaagcctt ccagcggcgt cattcccatt gaagtatcaa cactctgacc gttgcgaacc
     9301 gcgcagattg acgcgccatt gccaagatgc gccacaacca ggccggagtc atcttccgcc
     9361 agattcagca gcgaatgtgc gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca
     9421 taacggcgta cacctaactc ttcataatat ttccacggca ggccgtataa ataagcttcc
     9481 ggagccatcg tctggtggaa actggtatca aataccgcca cctgagttac gcccggaaat
     9541 aattgctgcg ccgattcaat accacttaaa ttggcgtaat tatgcagggg tgccagtgga
     9601 gaaacgcgac ggatattatc aatgacttca tcggtaataa tggcggactc ggtaaaaata
     9661 ctgccgccgt gagcgatgcg gtggccaatt aaggccacac tgtcatttaa attccgtttt
     9721 tccagttcaa atgcaattgc cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct
     9781 ggctctcccc catttacgga taagaatgca ttttccgagt taataccgtc ggcaatacct
     9841 gacattaata cttcacagtc gctggcatcg agtacggaaa acttaatcga agacgaacca
     9901 cagttaataa ccaaaacaac cggaaattca ttcatctctt ttctcatcct gagttacgga
     9961 ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga ccaatcacgg taacaaacac
    10021 gttatccaga cgaccacggt atttcgccag agacggcgct ttacggatgg catacatcgg
    10081 caacaggcac agcagggatg cgataatcgg tgcgcccatg gcttcaataa ggtcgaggat
    10141 gttcgggttg gcgtaggcaa caacccaggt ggagcccatg atgaagatca tgctgatagt
    10201 gttcagttta cccagcgaca ctttagtttt gtcgccttta taaccgaact tcagaatcag
    10261 accattcaag ccttccagcg tccccagata gtgaccgaag aaagatttga agatagccac
    10321 gagtgcgatg atggaagccg catattccag tgtaatcgcg aacgttgttt tggtaccggt
    10381 catggacgca aagtggttag ccagataaga aagcactgga atattctgcg ctttggcttc
    10441 cgccatgttg gccggagaca gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc
    10501 aaccatcagc atgctggcac gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg
    10561 accgaagtct ttctcatact cttcacgttt agaaaccacg aaggaagaga cgattggcga
    10621 gaagttaaag gagaaaacca tgatggaaat ccccagccag acagtgatca ggataccgtc
    10681 atgaccggtt aacgacagcg aaccgaggtc aacctggtcg ataactgcag agttccagta
    10741 agggatcagc gacagagaaa tcagcaccag gctggcgata aacggccata ccaggtagct
    10801 catcacttta accatcagat ccttaccaaa ccagatgacg aaagccatca gcagcaacag
    10861 gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg agctggtttt cccagaacgt
    10921 cataaaggta ttggtaatag taacgccata aatccacagc agtgggcaaa tcgcgaagaa
    10981 gtacaggaac gtgataacca cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt
    11041 aatgttgccg gaagggttag agccagaaag acacagacgc gccagcgccc ggtggcaata
    11101 aaatgcgatg gggtatgcca ataccaacat cagaagaatc gggatcagtc cgccaaaacc
    11161 tgcgcggata gggaagaaca gcaccccggc gccgattgcc gtaccaaaca agcctaacgt
    11221 ccatgtggta tctgatttac gccaggacga ttgttttgtc tggctggata caatgctatc
    11281 tgaagtactc atatcctatc ctcaacgaat taattaagcg tcaacgaaac cggtgatttg
    11341 agagacgcga gaaagatcga tattgccgcc ggaaataata ctgacggttt ttctgttttg
    11401 aatatattgg tctaatttac cgcttaataa tgcagcacat gccagagcgc ctgcgccttc
    11461 ggtgacgact ttattgcgct gaattaaggc aatcatactg tttctgattt cgtcttcgct
    11521 gaccagcacg atgtcatcga ctaattcacg aacgatttcg taagttaaat tacccgggcg
    11581 ggagacatca caaccatccg ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga
    11641 gtggaaagaa gccgccatgc cgtgaacgtt ttctgactgt acgccaataa cacgaatggt
    11701 cgggttaata gatttaattg ccaccgcaat accagcaatt aaaccaccac caccaattgg
    11761 cacaatcacg ttatcgacat catagagatc ttccataatt tccagaccaa tcgttccctg
    11821 gccagcaatc actttcggat catcgtaagg tgggataaaa atacgacctt ccatttcgac
    11881 aatttcgctc actttagcga tagtgtcgtt gaaattatca ccatgcagaa cgacttctgc
    11941 ggagtagtcg cacgttgccg ctactttgga ttttggcgca ccttttggca tcaccacttt
    12001 accgtcgata cccagcatcg cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga
    12061 acaggccact acacctttac gtttttccgc atcaatcagt gaacttaatt tattaaatgc
    12121 gccacgaatt ttaaatgaac ccgtacgctg catattttca aacttcagga atatttcacc
    12181 tttgcaacgt tcactaaaat agtttgagcg aggcatgcct gttttataaa ttcgcccagc
    12241 cagtcgttgt ttcgcttcaa taatgtcatc aatagcaacc ggcagatcgt atgtaatatg
    12301 cattataaaa cctcttcgcc tgaattaaat atgtaggtaa ccgtaaccga cacctgcaaa
    12361 acagacaggt ggattattta tgtttaaata aaaacaaata actaaccaac ttctattaat
    12421 tgccttcgtc tacacccatt ataagatgaa tactctttgg ctaattccac caaaaccgat
    12481 gctgcttttt taatacgata atttttcgac cataccgcgg catattgtgc cacaggtaat
    12541 gtttcttcaa ccggaatagt aataaattga tttgaaccaa aaggtgacgt catatcacaa
    12601 ggaattacag ttaagaaatc agcattgaga acaagattat aaattgtcac gactgagtcg
    12661 gttttaacga tgttttcaat actgatgcca tttctttgta acgtagtaag aagctcgctg
    12721 tagtacccca tattggtttg tggcaacacc cactgttcgt tcttcaacga ctccagcgtg
    12781 gtggtgccgg tgcatgttcg ggacttactg gctaccaaca caaacccgga ctcgaacagc
    12841 ggctcaacat gtaaatcctg aagcttcatt tctgcactta acgtaccaat cgcaaaatcc
    12901 agtcgaccgt cgcggattgc cggtaagaac gaagacagtt gcgcttcata catagaaact
    12961 tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc ctgacataaa agtaaaacca
    13021 atcaatgaag gaaaaccaaa tgagacttcc accaccgcct cagaagacat accacttatc
    13081 tcattaacca tatttttcat ttcacgggta atggattcgg aacgggagag taacaattga
    13141 ccggcaggtg ttaatgttac accggtattt ttccgcacca ctaattccac accaaaataa
    13201 tcttcaatat cgttaatgat tttactgacg gctggttgag ttaaccctaa ttcttttgca
    13261 gccgagccga tagagccact tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc
    13321 ggaagaagaa tagtgctcat aacgacctac gttaattacc tcattgacgg catgaagtgt
    13381 atcaaaatga aatgaacagg atatgtgcga ccactcacaa attaactttc aatactttcc
    13441 agggtatcgt tattacaatt aaacaccttt aaatatcaac aagttaaagc ataaaaat
//
LOCUS       DAZFWP020000131        13431 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFWP020000131 DAZFWP020000000
VERSION     DAZFWP020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.131"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..1099
                     /locus_tag="ACHK0Z_RS18670"
                     /old_locus_tag="pgaptmp_003734"
     CDS             41..1099
                     /locus_tag="ACHK0Z_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU4503686.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1109..2251
                     /locus_tag="ACHK0Z_RS18675"
                     /old_locus_tag="pgaptmp_003735"
     CDS             1109..2251
                     /locus_tag="ACHK0Z_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU4503687.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2244..3017
                     /locus_tag="ACHK0Z_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             2244..3017
                     /locus_tag="ACHK0Z_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503688.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3019..4098
                     /locus_tag="ACHK0Z_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             3019..4098
                     /locus_tag="ACHK0Z_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU4503689.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4098..5054
                     /locus_tag="ACHK0Z_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             4098..5054
                     /locus_tag="ACHK0Z_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU4503690.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5065..6273
                     /locus_tag="ACHK0Z_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             5065..6273
                     /locus_tag="ACHK0Z_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGU4503691.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6291..6758
                     /gene="terW"
                     /locus_tag="ACHK0Z_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             6291..6758
                     /gene="terW"
                     /locus_tag="ACHK0Z_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU4503692.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7019..7348
                     /locus_tag="ACHK0Z_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             7019..7348
                     /locus_tag="ACHK0Z_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4503693.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7335..7715
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS18710"
                     /old_locus_tag="pgaptmp_003742"
     CDS             7335..7715
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4503694.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            7758..8588
                     /locus_tag="ACHK0Z_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /pseudo
     CDS             7758..8588
                     /locus_tag="ACHK0Z_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8619..10232)
                     /locus_tag="ACHK0Z_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             complement(8619..10232)
                     /locus_tag="ACHK0Z_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HGU4503695.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10263..10613)
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS18725"
                     /old_locus_tag="pgaptmp_003745"
     CDS             complement(10263..10613)
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4503696.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10610..11035)
                     /locus_tag="ACHK0Z_RS18730"
                     /old_locus_tag="pgaptmp_003746"
     CDS             complement(10610..11035)
                     /locus_tag="ACHK0Z_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4503697.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11089..11840
                     /locus_tag="ACHK0Z_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /pseudo
     CDS             11089..11840
                     /locus_tag="ACHK0Z_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            13223..>13431
                     /locus_tag="ACHK0Z_RS18740"
                     /old_locus_tag="pgaptmp_003748"
     CDS             13223..>13431
                     /locus_tag="ACHK0Z_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503698.1"
                     /translation="MEFSVIFFWVITGLITILFLKSIITDLIKNLNKTDIALYILAFL
                     SAGYTFQYIYNDNIFSELWGVGLFVT"
BASE COUNT         3249 a         3511 c         3488 g         3183 t
ORIGIN      
        1 caatcataaa tttgcatggc gttccggata attaaaaaaa atgaacaaga aaatttggtt
       61 tatggaaggt ttatcctccc agcgagatat tattcagggg gtaaaatcat ttgcacaaaa
      121 aaataatttt gccattaccg tttttgcctc ccaccgtaac gaaagaaatg aaatcctttc
      181 cgttgccgat tattctttga ctgaacctga agatcctcaa aaacgtcttc agtttatcca
      241 ggaaaccatt cagacctacg gcatccacca tattcatact ggccgtaaca gccagtggtt
      301 tgaagaacac cgttcagcca ttgaaccgac cggtgccacc cttactaccg gtgcaacggg
      361 cgtcgactgg ttaactctgg ctgacgaaaa agttactttt gctcagttta tggagcaaaa
      421 gggtctcccg gtcgtaccat cctggcgggt gaatacgctg gcagaattaa agacacacct
      481 cgcggccccg ccgttcactg acagcccggt atgcgtgaag ccggtgacgg gtatctatgg
      541 catgggattc tggcgctttg atgacagtgc ttcgcctatg gccgtcttta atcatcccga
      601 acatcgtctg gtcagtccgc aacagtatat tgcagcagca tcagctgctg agtcgtttaa
      661 accccttgtt ttgatgccgt acctgccagg cccggaattt tccgtcgata tcctcgcgga
      721 taagggcgaa atactcgcag ccgtgggacg ccgtaaggaa ggggctatcc agtatctggt
      781 aaacgaagga agcgcctggg aactggcgtg tgactgcgcc cgtgttatga aggccgacgg
      841 gctggtgaat gttcagacgc gaaacgatgt gaatggcaac ccggtgctgc ttgaaaccaa
      901 catgcgtccg tcaggggggg tgggttatac ccttcacagc ggtgtgaacc ttcctgggtt
      961 atttgctgcc tttaagctcg gtctgatgtc tgaagatatg gtacgccaga gcgctaaaaa
     1021 caccttttct ccggttgcgg tgagatccat tacggatgta attgcatacc cggaatcact
     1081 ctctaacctt ctgaattaag gcgtatttat gtctgatatc ctcgaagata tgatttatcg
     1141 ccgtaccctc tcatgcggta cgattcaggt aacccgtgac cagggtgaag tttcgctcga
     1201 tgacctgttt gacatcgctg aaagacgtaa cccgaaacgc gcctttctgt ttgtcagcaa
     1261 agtactcggc aggcacattc ccgtgccacc ctcagtcatg cgacaggcct acagacagct
     1321 tgccagccag ttcccctcga cactgacagg acccgtactg tttatcggta tggctgaaac
     1381 cgccgttggg cttggggccg gtgtatttga tgaagtgcgc caccagcatc gtgaatctgt
     1441 ctatctgacc tctacccgtc acccggttga tggcacgttg ctttgcgagt tcaaggaaga
     1501 acacagccac gccaccgatc atctgatcta tctgccagat gatgaagaga aaagacgtcg
     1561 tgttaccaac gcacgaacgc tggttttgat tgatgacgag gcaaccaccg gtaatacctt
     1621 tattaacctg ctttctgccc tgcgtaatac cggcaagctt caacatattg aacaggttat
     1681 cgccgtaacg ctgaccgact ggagtggcaa tgccctgtcg gagcgcaccc ccttgcccgt
     1741 cacttcggtt tcgcttgtaa gcggtcagtg gggatggacc ccattacctg atgcacctgt
     1801 cccggacatg ccgaaagtca acgtaacttc acgaggagaa tgggacatcc aggggaaaca
     1861 gtcatggggc cgactgggga tgctcgcacc tgcggccgat ctcggccatg aggtctccgt
     1921 ccacaagggg gaacgtattc tggttctcgg gaccggggaa ttcgtctggg agccgttcct
     1981 gcttgctgaa cggctcgaag ctgccggagc acaggcattt tatggatcga ccacccgctc
     2041 ccctgtcgcc gttggttatg ccattgagtc cgccatttcc tttacggata actacgggct
     2101 gggcatcccc aattttgtct ataacgtcgc ccaccagcag tttgaccgca ttcttgtgtg
     2161 tactgagaca cccgcagaaa gtattgacac gcagcttctt aaagcgctgg ctgaggttgc
     2221 gcccgtcgtg gagattgtta cctatgaata aacccgttgt tctgagcgac cttgacgaca
     2281 cgctctttca gacccgtcgt aaaatggtgg acgagctggc cctggagcca ttccgtaccg
     2341 gtgccgttga tcgcaccctg aatccacgaa gctttatgac ggaagaacag tccatgttgg
     2401 tggactggct cctggagcag gctgaactca tacccgttac cgcacgggga actgaagaaa
     2461 tcagccgcgt acggatccct ttccactcct gggcaatcac aacgcacggg gccgtcattc
     2521 tcacgcctga aggcaaaccc gacgaggagt ggaaggccca tatgctcggc cagctggctc
     2581 cctaccagga aaagctgaca tcgatgcagc gtctgatcac tgaaatgatg gacgcaaaag
     2641 gaatcaatgc ctgggcaagg ctgaacttcg aatacggtga aacggcggtt tatatggtga
     2701 tgaaacaccg cgacagcacc cgccttgacg agctcaatgc cattgcagat gagatagaaa
     2761 cggtgtttcc gaccgagggc ttctacatcc accgcaacag taataacgtg gcctggcttc
     2821 ccaccccggt tgagaaaggg ctggcagtca gctggctgct tgaaaaactt cgggctgaac
     2881 gaggagtttt ccccgtaatt ggtttgggcg acagcctgag cgatcatcgt tttatgaaac
     2941 tgtgcagctg gtttggcatc ccgcgtcaga gtcagtttgc agatgccatt tcacagcgaa
     3001 tttttggaga aaattaaaat gcagccacat gaaacattta ccggctcata ccagcccggt
     3061 gacgtggaat ttctgctaaa gccggttgtc attgagatga cgccggttga gaaaaaagaa
     3121 gaactgattc agtccgggaa gaaacattat tcggacatgc tcagccagga gccagcgcct
     3181 acacaatggc atcttgattt gtttcaccgg gcgctggatc ggggcgcaga gaggcttgca
     3241 aaagaagtca cacagctcgc tattgctctg gccgaacgct tcggtgatga gcccattgta
     3301 ctggccagtc ttgtcagggc tggcgttccg ctcggcgtta tgctgcacca ggccctgcgt
     3361 gacatgggga aaacctcatg gcattacggt atcagcatta tccgggatcg tggaattgac
     3421 ggtgcagccc tcgacgtcat tgaagagcgg catggtacca gcggtattgt ctttgttgac
     3481 ggatggacag gtaaaggcgc aattaccgga gagcttgtac gcgcactgaa agatcgcccg
     3541 ggctatcctg agcagccgcg actggttgtc ctggccgatc cctgtggctg ctcctggctt
     3601 gcagccagtg atgatgactg gctcattcct tttggcatca tgggagcgcc ggtatcaggc
     3661 ctgatctccc ggtcagtatg gtcctctgaa ggattacacg ggtgcatggt ttgcgagcat
     3721 ctcagtgaat tcgaatgcag ccggatgctt gccgataccg tcgctcattt ccgtaagaag
     3781 ttaacaccgt catccctcgc tcccctgagc tggaatacgg aatcagcccg ggtcttatgg
     3841 cagacaagtc gcgacgttat cgcgttcctg gccgatgaat ttaaagtgga cagcgtcaat
     3901 cgtattaaac ccggtattgc tgaagcaacc cgggctgtat tacgtcgggt accggaccat
     3961 gtatttgtgc gttctattga cgacccggac gttgccttgc ttgtagggct tgctcgtgaa
     4021 aagggaatag ttgttacaga aatgggggga accctcggcc agtatcgggc tgtaaccatt
     4081 atcaagaagg tactctgatg aaaaatcgtc tttctccctg gaatctggga gccacgctat
     4141 acatgcctgc aacacgggaa gatattgctg atgccgtcct gcacgggaaa atcccgggct
     4201 tgcgctctct ggtgatttgc ctggaggatg ctgtcagtga agcagacatc cccgtcgccc
     4261 tcaaaaatct ggagcacctg ctgcacgagc tcagtaacag catgagtagc ctgggtaaaa
     4321 atgactggcc tctggtgttc attcgccccc ggcatgccga aatgggcaga tggttaaaag
     4381 cgcattatga tctttccgct gtcgacgggt ttgtgctgcc taaatttacc ctcagttcgt
     4441 tagctgaatg gtgggatatc atggtcggga ctcacctgtg catgatgcca acgctggaaa
     4501 cagaagatgt ctttgacgtg gttcagatgc gcgagctggc tacccgtctg gaggaacatc
     4561 cctgccacga ccgcattatt gcgcttcgaa tcggcggcaa cgatcttatg aacgttgtgt
     4621 cgcttcgccg tccccgggac ctgacgctgt atgacagccc gatgggctac gtcatcaaaa
     4681 tgctcgtttc ggtcttcggc ccgcgtgatt ttgccctgac cgcgcctgta tgtgagcata
     4741 ttgacgatca tgccgttatg gccagagagc tggctcttga tatggcacac gggcttgttg
     4801 ggaaaacggc cattcacccg ggtcagatag aggtcattca aaacgcgctg atggtcactc
     4861 agggtgagca ttctgacgct ctgcggatcc tgaactctac ccaggccgtg tttaagtcgc
     4921 agggagcaat gtgtgaaccc gccacacatc gccgctgggc ggctggcatt ctggacaggg
     4981 ctcgttttta tgggttacag aacgagcaaa gcgctgatgg aatcagatta cttaccgtga
     5041 cccagcatca ttaaggaaat cataatgggg ttcaggttca gaaagtcgat caacattatt
     5101 cctggcgttc gcctcaacct gagtaacggt gcaccgagcc tgagtgtcgg gccgagaggt
     5161 gcttccgttt cttttggtag ccgggggacc tatgccaatc tgggcttgcc cggtaccggg
     5221 ctgagttacc gtacccggct tgaccgggcc gcgcgttcca gaggtgaaaa ccggacggca
     5281 accgacccgg ggctcagaca ggcgcttgag cagaaagccg ctgaactcat gtcagcggta
     5341 accgcaatcc gtaatattca cgagctgacg ccggatccaa aaacaggcat cagctgggca
     5401 gagctggaag cagtatacct gcataacaga acgtcgcctt ttcaggttcc ggcaccggtg
     5461 cgtccagaaa agccagacta ccttgtattg ccggaaaagc ctgccgagag cgaaggcatt
     5521 agttttttgg gtaaatggtt tgaatcggaa tcagctaaag ctgagcgcca cgccgaaaat
     5581 cttcgccggt ggcagcagga gctgattgat gtggagcgtg agaataccct tcgacagcac
     5641 cggtaccagc aacaacggac ggcctgggcc gaacagtatg caaactggaa gtttgaagct
     5701 gaagaacatg aaaaacggct cgccacggct caggcagatg cccggcagca gttccggaca
     5761 gacgccgcgt ttttcgaatc atacctggcg ggtgtgctgg cagaaactga atggccgcgt
     5821 gaaacgcttg ttgcatttga agtaaagccg gagctatcag cagtcctgct ggacgttgat
     5881 ttagctgaga ttgaagattt ccctgataag atttacggcg ttaatgcccg gggaacggag
     5941 ctgacggaaa aagccatgac gcaaaaagcc gtacgcgaaa actatgccca ccacgtccat
     6001 ggctgcttgt tccgcctggt cggtatcgtt ttacatacgc tacctttcga caacgtgatt
     6061 gtgtcaggct ttacgcaacg ggtcagtaag cggaccggct atctggagga tgagtatatc
     6121 ctgtcctgca aatgcactcg cagccagatg tcgtcagtaa attttgcagg cataaaacac
     6181 attgatccgg ttgaagcgtt aggcgatcaa ccggttattc gaaagatgag cagtaccttc
     6241 atttttcagc ctattgaacc actaaccctt taagcaggct aaatcttacg atgcaattaa
     6301 ataccagaca ggcccggatt tttaagcttg ccaacttact gggcaccggc aagcccgttt
     6361 ccgctgccga cattataacc agtctggaat gctctgagcc gacattaacc cgggccctca
     6421 aagagctacg cgagtcatat tctgcggaga tcaaatacag caaggcaggt cattcctatc
     6481 atctcgttaa tccgggccag ttggataaaa agaccctccg ccggatgaat gaagcgctcg
     6541 cacagaatgc tgaactgaaa accggtgagt ccacagggaa ggtcgtactc gataaggata
     6601 aaaaaacagc cgtgtcatta tcgctacgca tgcggattct aagaaaaatt gaccgtctgg
     6661 ctgcgctgag tgactcgacc cgaagtgaag cggtagagaa gctggctctg cactccgttg
     6721 atgaactgat aaaagaatac agcgctaaaa agtcctgacg ctaaatctca gaccggggtg
     6781 aaaactccgg ttttgtcata tttcacctct gtattggcgt gatgcaaaat ggcatctgaa
     6841 tcgatcatgg tttggcatcc atttccgatc acagtttggc atcacaatcg atcacgattt
     6901 ggcatgcttc cgctcattga ttggcatcct ggtgtcgtta cggggaagtc atcttcacag
     6961 ggatcttcag tcagttagca ttaccgacat ccattcacca acttaccgga gataatcaat
     7021 gtcaaaaccc cgatggacgc ttgatcagaa aaaacatcac gttgccgcat ggcgcgccag
     7081 cggccttacc cgtgaacagt actgtgaact ttacgacatt ccgtttaaat ccctgcgcca
     7141 gtggccgcag gatgtcgcaa aagccgaaaa acgagccaga gcaccagaaa ttattcctgt
     7201 ttcagtatct ggctcttccg gaatgactga tggcagacca ttgtcggatg agcctgtcac
     7261 tctcttcctg cccggtggta tccggatgtg ctgccagcct tctcagttaa ccgatgtttt
     7321 ccgggctctt cgccatgctg acgcctgata acgtattcct ggtcgtaaaa cccgtcgata
     7381 tgcgccgggg tatcgacaca ctgacacagt acgtccagaa cgaacttaac gctgcatggc
     7441 atgacggtgc tgcttttgtc ttcaccaata aagtccgctc ccgcataaaa gttctccggt
     7501 gggacaaaca tggcgtatgg ctgtgtaccc gacgactgca ccggggaagc ttccgctggc
     7561 cccggaaagg tgatgccaca tggcatctca cacaggatga gtttcactgg ctggtctttg
     7621 gggttgactg gcaacaggtg aaagggcatg acctggcaaa atgggtatac caggaagaac
     7681 ccgaagatgc tcagagcaac gcaataactc aatgaaatat aatgaatatc catgcgtgat
     7741 gtcatataat ccaccgcatg gatatctcgc ttctcagtac caccagtgac cctgaacagc
     7801 ttcgcgctct ggcaattgcc atggtgcaaa aagtcatggc tgagaatgca gaacttcaga
     7861 accgtatccg catcctggaa gagcagatga agctcgcacg ccagcagcgt ttcggtaaaa
     7921 aatgcgaatc gctggccggg atgcagcgtt ccctgttcga agaggatgtt gacgcggata
     7981 tcgcagaaat ctcggcgcat ctggacaaac tgcttcctca gaccggggac gaagaaaaaa
     8041 caacaacacg tcctgttcgt aagcccttgc cttcacctct tccccgtgct gaaaaggtta
     8101 tcccgccagc tgaggaacgt tgtcctgact gtgatgcccc cctgcacttc attcgcgatg
     8161 aagtcagcga gaagctggaa tatattccgg cacaggtcgt ggtcaaccgc tatatccgcc
     8221 cgcagtacag ctgtccctgc tgtgagaaag tgttcagcgg aaaaatgccg gcacatatcc
     8281 ttccgaaaag tgctgttgag ccatcggtca tcgcacaggt tgtcatcagc aaatataccg
     8341 accatctgcc cctgtatcgc cagcagcaca ttttctcccg aatgggggtg gagctccccg
     8401 tcagtacaat ggcggatatg gtcggtgttg ctggcgctgc actggccccg ctggcaaaat
     8461 tgttacgcca cgagcttctg acacgggatg ttattcatgc agatgaaacg tccctgaggc
     8521 tccttgatac ccggaaaggc ggtaaatcct gctccggctg gctgtgggct tacgtcagcg
     8581 gagaaagagt aagcgtcaac ggagcaccgt attgacgctt atttattggt gagtactacg
     8641 ttccatggca ggagttcatc aactcggttg gaaggccatt ctggcagtac gctcaggata
     8701 tggcgcagat acgcttccgg atcgataccg ttcagacggc aggtgccgat cagcccgtac
     8761 aacagtgctc cacgctcgcc gccgtgatcg ctgccaaaga acataaagtt tttctttccg
     8821 agacagactg cacgaagcgc tctttccgca gcattattat ccgcctccgc cagaccgtca
     8881 tcactgtaat aacagagggc atcccactga ttcagtacat agctgcacgc ttcgcccagt
     8941 ctggattttt tcgacagcgt accattcttc tccaccatcc attcatgcag tgacgtcagt
     9001 aacactttgc ttcgctgctg cctgacggca agacgctctg actccggtaa tccccgtatt
     9061 tcatcctcga tggcgtacag ttcactgatt cgcttcaggg cttcttctgc cgtcgcactt
     9121 ttgctgctga tgtatacatc gtggattttt cgccgggcat gggcccagca cgcaacttct
     9181 gtcagtgcac caccttcacg ttctgcactg aacagccgat cgtaaccgct gaacgcatcc
     9241 gcctgcagga taccccggaa gggacgaagg tgttgtaccg gatgttttcc ctgcctgtct
     9301 ggtgagtagg cgaaccagac cgccggtggc tctgatgagc ccgcattccg gtcatcccgg
     9361 acatacgtcc agatgcgtcc tgtttttgcc ttttttctgc ccggtgccag cacttttact
     9421 ggtgtgtcgt cagtgtgaac cttgcgggtg ttcatcacgt aacggtacag ggtatcattc
     9481 agcagagtca ttaactgaca acatgcgtca acccagttgg agagtaatgc ccggctcagt
     9541 tcgatgccct ggcgggcaaa gatttcactc tgacgataca gtggcaggtg ttcgcagtat
     9601 tttcccgtta acacgcgggc aagtaatccg gagcccgcga tgccgcgctc tatcgggcgg
     9661 gacggcgctg gcgcttcaac tatacagtca cattttgtac aggctttttt tacccgaaca
     9721 gtgcggatca ctttcagggc gctactcacc agttccagct gctcagcact gacttccccc
     9781 agataatcca gctcactgcc acactccggg caacaacttt cttcaggctc cagacggtgt
     9841 atttcacggg gaagatgtgc cggtaacgga cgacgatgac gtgactgtcg caactggcgg
     9901 ggaacctgcg ggtcatcctc acgcccactg taacgatcgc tttcctgttc gcgttgtttc
     9961 agttgggcct cagcctgttc aacctcacgc tgcagttttt cagaacgggt accgaacaac
    10021 atccggcgca gtttttctat ctgggccctc agatgttcta tttcccgctc ctcctcttcg
    10081 atcttttcct cggcacgtgc cagtgcagag cgcaggaagg cctccgtctc ttcaaccaga
    10141 ctcagttgct gatctttctg acggagggct tcagcctgct cagagagcag cctttccagc
    10201 tcagtgatac gaatgaggta tttccgactc atgaccgttt ttataatccg gccatgacat
    10261 ttttacaaca ttgtcagtgc attaaggcgg gatgttttgg gctgacgcca gtccagttta
    10321 tcgaggagca ttgccagctg cgagcgggta atggatacct taccgtcacg caccgcaggc
    10381 cagataaact ggccttcctc cagacgtttg gtgaacaggc acagaccatc agcatcagcc
    10441 cacagaattt taatcgtgtc accccgtcgg ccgcgaaaga taaacaggtg accggagaag
    10501 gggttctcat ccagcacatg ttgtacctgt tctcccagac cgttgaagga tttacgcata
    10561 tcagtaacgc cggcaaccag ccagattcga gtgcctgatg ggagcgagat catcgtcccc
    10621 tcccggtcag ttcacggatc aacaccgtga gcagctctgg tgaaggattt tccagcgtca
    10681 tgttaccgtg gcggaactca actttacagg aactggcact gactgtgctt tgtgaaggag
    10741 tggataaaag cggagtaaga gccgccatag gctctttctg ctcatcaggc gttatctcaa
    10801 caggtaataa ttcaacgcca gcgccagaag aggttgttac cggaagacgc cgcgatatac
    10861 gcctttcgtt ctgccagagc ctgagccatt tgaacaggag gttatcattg atatcgtgtt
    10921 cccgggcaat acgggcaaca gaggctcctg gttgtgaagc cagtttaacc atttgaagtt
    10981 taaactcatt tgaaaatgat ctgcggggtt ctgaggataa tattttctgt tccataacag
    11041 gtgtccacta gttgaaaagg tgggcaccta cgttaccaat actggcttaa tggctacata
    11101 cggcggtcag tttacgctta cgagaaagaa gcggtccgcc ggtcgtctgt tttgactcgc
    11161 agaccggacg ggccctcaga tatcctgagg cctggctgca gggctggtgt ggcggtacgc
    11221 tggtgagtga cggatacagc gtgtacaaat ctctggcgga taatgacccc ggtattacat
    11281 ctgcctgttg ctggagccac gcaaggcgcg gtttcgcaaa tctgtataag gccagcaggg
    11341 agccccgtgc agccatggcg ctgagaaaaa tcgcgggtct ttatcgcatt gagaagttca
    11401 tccgtgaacg tcctgtggaa aaaatacggc aatggcgaca acggtattcc cggccgatag
    11461 tgaatgacct gtttgcctgg cctgaagagc aggagccgtg ctgtccgccc gacggaccac
    11521 taaacaaagc gattaattat atcctgaacc ggcgcgatga actgagctgc ttcctgagtg
    11581 acggagccgt gcccctggac aacaatatct gcgagcgtgc tatccgcccg gtggtcatgg
    11641 ggcggaaagc atggttattc gccggttcac tgatggccgg gaaccgcgcg gcacagataa
    11701 tgagccttct ggaaacggca aaacgtaatg gtctggagcc gcatgcctgg ctgacagacg
    11761 ttctgacgcg tctaccggag tggccggaag accgactgga agagttgctg ccccttgagg
    11821 gatttacctt ctccgggtga gtgatacctg ccatcaggtg ttcgtgcagc gggccataac
    11881 ccgcagtcgg gagttgaacg cctgacgaca ggaaatgagc cagagcagcg accacgcggg
    11941 ctgacagtga gtcagaacct gaatcagccc ggagccattc ccggggctgt acttcatcac
    12001 ctgccatcag tacccgtgtc agtacttcgc gacctgacgc aattacccga ctcagttctt
    12061 cagaacccgc ctccgatacc agcctggcgg tcgccccggc gcaacagaat aaatcaacca
    12121 tcgtcagact gcgatcattc gtatgcatcg ctgatgatgc gcccgaaaaa cgcgtcaata
    12181 tgagttcgcc ggaatattac tgacgacacg gtttgtagtt acgttccttc ggtgagtgag
    12241 tacctgtgcg tcagggtaac gcaatcagtt caggtttagc ggggtgtcaa gtcggtttgg
    12301 ctaaccgacc gcagaattaa ctgtaccttt aaaggtcgcc cccgatatat ctgcctgtca
    12361 gacatatcgc cgattcggta tctcactaca taaaactcct taaaaaacaa tgcgattctg
    12421 ttggtacgaa gctttcagta agtcagtccg gagaagcgcc agtacgatac agaatgaggt
    12481 tgaaaaaaac gttcacgtct ggtcacttct tcaaatgtta aaaaacatct cgtaaagagc
    12541 cagtttatgc gggttcgttg agtagacgtg cgagcgtcac tttgtcagct ttaacgctga
    12601 cgaaagtgac gcccgcacag cttcaaactc cacatgcaca cgcaagcgac caaatttcaa
    12661 atggtcttat gccgtgcgca cagtcataag aagcgcatgc ggaaagtcag aaaatatctt
    12721 aagacgcaga ttttaaaggc gtttttatgc cagtctgccg ggaatacaca gtagtccggt
    12781 tgtccggaag caccacggta atccgaagga aaaatcaacg tacctcaatc accgtagtga
    12841 tatccgttcc cagaggctaa acggatgtac caccataaag gcatcctctt tcaggctacg
    12901 aggatattgc aatcacccga aggcgtttct ctcagattgc tgaaacattg gctggcagtc
    12961 acccgaaggc gtccctctca gactgctgga acattggctg gtcgcaaaag cgacagttaa
    13021 tgcaaataca ggttaacaaa aagggaaatc tgccgtcaac ccaaccatat gaacatgttg
    13081 atttcattat gaatgtgatg ttctaatata aattaatcag taacttcata aaaagaatat
    13141 aagaaacaga taatgcatgt atttacacac atatcagcac aatgatagat taccacccca
    13201 gaaacacgat aaaggtataa ttatggaatt ttccgttatt tttttctggg ttatcactgg
    13261 tcttattacc atcttattct taaaatcaat aataaccgat ctaataaaaa acctaaacaa
    13321 aacagacata gcgttatata tattggcatt tttatcagcc ggatatacat tccagtatat
    13381 ttacaatgac aatatatttt cagaactatg gggagttggc ctgtttgtta c
//
LOCUS       DAZFWP020000132        13396 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFWP020000132 DAZFWP020000000
VERSION     DAZFWP020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.132"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..617
                     /gene="sxy"
                     /locus_tag="ACHK0Z_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             <1..617
                     /gene="sxy"
                     /locus_tag="ACHK0Z_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU4503699.1"
                     /translation="YKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVSDGELY
                     LRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYSLDAAL
                     KEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQNSLVTE
                     KILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(580..2742)
                     /gene="yccS"
                     /locus_tag="ACHK0Z_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             complement(580..2742)
                     /gene="yccS"
                     /locus_tag="ACHK0Z_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU4503700.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(2752..3198)
                     /gene="yccF"
                     /locus_tag="ACHK0Z_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             complement(2752..3198)
                     /gene="yccF"
                     /locus_tag="ACHK0Z_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU4503701.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            3321..5375
                     /gene="helD"
                     /locus_tag="ACHK0Z_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             3321..5375
                     /gene="helD"
                     /locus_tag="ACHK0Z_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU4503702.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(5407..5865)
                     /gene="mgsA"
                     /locus_tag="ACHK0Z_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             complement(5407..5865)
                     /gene="mgsA"
                     /locus_tag="ACHK0Z_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU4503703.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5961..6623)
                     /gene="yccT"
                     /locus_tag="ACHK0Z_RS18770"
                     /old_locus_tag="pgaptmp_003754"
     CDS             complement(5961..6623)
                     /gene="yccT"
                     /locus_tag="ACHK0Z_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU4503704.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6796..7209
                     /gene="yccU"
                     /locus_tag="ACHK0Z_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             6796..7209
                     /gene="yccU"
                     /locus_tag="ACHK0Z_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU4503705.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7254..7571)
                     /gene="hspQ"
                     /locus_tag="ACHK0Z_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             complement(7254..7571)
                     /gene="hspQ"
                     /locus_tag="ACHK0Z_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU4503706.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7629..8819)
                     /gene="rlmI"
                     /locus_tag="ACHK0Z_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             complement(7629..8819)
                     /gene="rlmI"
                     /locus_tag="ACHK0Z_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU4503707.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEDGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8914..9192
                     /gene="yccX"
                     /locus_tag="ACHK0Z_RS18790"
                     /old_locus_tag="pgaptmp_003758"
     CDS             8914..9192
                     /gene="yccX"
                     /locus_tag="ACHK0Z_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU4503708.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLVQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(9189..9518)
                     /gene="tusE"
                     /locus_tag="ACHK0Z_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             complement(9189..9518)
                     /gene="tusE"
                     /locus_tag="ACHK0Z_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU4503709.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAKNEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(9609..10268)
                     /gene="yccA"
                     /locus_tag="ACHK0Z_RS18800"
                     /old_locus_tag="pgaptmp_003760"
     CDS             complement(9609..10268)
                     /gene="yccA"
                     /locus_tag="ACHK0Z_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU4503710.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(10676..11695)
                     /locus_tag="ACHK0Z_RS18805"
                     /old_locus_tag="pgaptmp_003761"
     CDS             complement(10676..11695)
                     /locus_tag="ACHK0Z_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4503711.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(11673..11915)
                     /locus_tag="ACHK0Z_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             complement(11673..11915)
                     /locus_tag="ACHK0Z_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4503712.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(11983..>13396)
                     /locus_tag="ACHK0Z_RS18815"
                     /old_locus_tag="pgaptmp_003763"
     CDS             complement(11983..>13396)
                     /locus_tag="ACHK0Z_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4503713.1"
                     /translation="NQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPF
                     YFLFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYLARKNGTYTDLPQNVDTAEDS
                     VQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIETCD
                     SVNQNQQESEQNDQKTEQNSPNAQQNEPETKQEPEKACTACGQTGGGSCPDCGAVISD
                     ATYQEIFDEENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANG
                     HHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAG
                     GVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGATF
                     DNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNALNDAR
                     YQAKYVSAIWQKLLPSQADF"
BASE COUNT         3371 a         3288 c         3397 g         3340 t
ORIGIN      
        1 cctataagcg gatctataaa tcacaagaat acctggcaac gttgggcaca attgaatacc
       61 gatcattgtt tggcagttac agcctgaccg ttgacgacac ggtgtttgcg atggtttctg
      121 atggtgagtt gtatcttcgg gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc
      181 ctgtctggct gacatataaa aagtgtggcc gatccgttac cctcaattac tatcgggttg
      241 atgaaagtct atggcgaaat caactgaagc tggtgcgtct gtcgaaatat tctctcgatg
      301 cagcgctgaa agagaaaagc acgcgcaata cccgggaaag actgaaagat ttgcccaata
      361 tgtcttttca tctggaagcg attctcgggg aggtggggat taaggatgta cgggcgttac
      421 gtatacttgg ggcaaaaatg tgttggttgc gactgcggca gcaaaacagt ctggtgacag
      481 aaaagattct gtttatgctt gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg
      541 tggcacgccg ccaggagctt gcagaatggg ctgactctct tacgccgaaa caggagtttc
      601 ctgcggaact tgagtaatct ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa
      661 taatccaact tgttgtacga ccagaggttc tttgctgtct gcccgtggtt caagttgctg
      721 catccgctgt ttcaggctag ctaatgcctc attgacgcgt tcttcatcag caggttgatg
      781 atgtaacgcg tcatcaacat agcaaactgc gtcatcaaga aacgccagaa tttcaggatt
      841 agttaactgc tcccggtgag caccgagggc tgagatatag ctggtaaacg tatggttaag
      901 gcacagcaac cgaaacgcgg cttcgcgaat ttgcggggta acgttcggtt cgctggacat
      961 atttgatacc accgacgcca gttcagcatc acggttgtgt gcatcgcggc gggcaatacg
     1021 atacgccaga cggttatcac gcccctgatg gtattgctcc agtatggcat cgagataccg
     1081 gcagttggcc tctgtggcgc gttcgagcat tcgcggcaga ttgcgaaact gccagtcagg
     1141 ccagatgtag ctcactgccg cccacgcaat ggcacaacca atcagcgtat cgattacgcg
     1201 aggtaacgct acttcaaaac cttcacccag caagttaaaa cacagtagca ccaaaagtgt
     1261 gatgaacatc gttgcatggg catattgcac gttacggaag gcaaaaaaga gcacgccggt
     1321 aataaccagt agcaccagct gcccttccag tgacggtaca aaccacagca caggaatgcc
     1381 aatggcgata ccgaccagcg taccaataat ccttaacttc aggcggtggc gcgtggcgtt
     1441 atagtttggc tggcagacaa acaaacttgt cagcaagatc caatacccgt gatgcattcc
     1501 ggttatctga atgatggcgt agccgaagca caacaccagc gacattctta ccgcatgacg
     1561 gaagagggcg gattccggcg tgaagtgacg gctaagacgc agccagatat cactcaatcc
     1621 gtgcgggcta tcatcagcga gctcattttc gtcattatta tggggcagtg cctgggcctg
     1681 ttctgattca attgttgcca gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc
     1741 cagtgtttta agtaaatcgg caggcgcgcc gttatcgcgc atccgctcca gcgcagcatc
     1801 aatatgcgta aaagcgcgct caaaatgcgg atcatgttga taaggctgac gcagcaaaat
     1861 acagcgtgac agttgctggc acgcctggcc ctgcatcgac atcagccgct gaaaacggaa
     1921 cagcacgtcg ctgtggcgaa aatgttcacg caatgtttga tactgaatat gagaagagct
     1981 ggcacgctcg tgaatatcct gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg
     2041 ttgaccacga tcgccacgta agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc
     2101 catcagctga ccgttggcga gagccaaatc gtacagcggt gcctggcttt catcttcaat
     2161 atcaggatca aacatgcgcg acttgagctc aagataacgc gccagttgtt catagcaacg
     2221 cgccaggttg tcctgcagcg ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac
     2281 gttgtaccag acggcaccgg ccagcagata catcggctgc tgataccagt gctcatacag
     2341 tgatgttccc aacatagtgt aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata
     2401 gcgttgaccc agaccgccga gcaaaatgaa gccgctggta gagagcgtta agccaatcgc
     2461 aaatagccag ggccagggaa acagcaattc tactgaggcc gaggcgataa aaaagcagaa
     2521 cagcgtaatg atgaggttac gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc
     2581 cgctgccacc atccccagcg ttagcggaat cgtcagtttt acatcaccca gccaccacgg
     2641 aaacgctgtg gttccacaaa gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt
     2701 ccaggtatag cgtttgagca aaggacttag cataaaggcc atattagtgg attattcaaa
     2761 acgacgacgt gcattggctt cacgcgcagc ttgcgccgtt tctaccgata ccacgcgacg
     2821 accaaccggc catagtgcaa tagcggcaat tttaaagttc gcaatgccga caggaatgcc
     2881 aatgattgaa atacattgtg cgatgcccgt tgcaatgtgc atcaggcata accaccagcc
     2941 aaagaaaatc agccagaaaa tattcaatac cgtaccgcca gtattcagca gcacattttt
     3001 gccagccggg ttcagttcat cgacatgaat agcttcattg ccataaggca ccagagacag
     3061 tttagtgatc tcccagcagg atcgtgtcag cggtaaggta aaaatcagca taatactgac
     3121 cagagtcgcc aacagccagc ccagagtggt ggcaaatccg ccaagcacaa agttcagaat
     3181 gttcaaaacg gtacgcataa aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt
     3241 gtaacgtttt tttgggctgg agcacgtttt ctctgacggt tacactgata agaaataatt
     3301 tcgtgtggat ctacagagtc atggaactga aagcgacaac gcttggaaaa cgtctggcac
     3361 agcaccctta cgatcgggcg gtgatcctca atgccgggat taaagtctcc ggcgatcgcc
     3421 acgaatacct tattcctttc aatcaattac tggcgattca ctgtaagcgc ggtctggtat
     3481 ggggcgagct ggaatttgta ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat
     3541 ggggcgagac gcagcgtttt taccatcatc ttgatgctca ctggcagcgg tggagtggcg
     3601 agatgagcga aattgcgtct ggtgttttac gccagcaact ggatttgatt gccacgcgca
     3661 ctggagaaaa taaatggctg acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc
     3721 aggctatgtc ggcgttgccg ttgccggtta accgactgga agaattcgat aactgccgtg
     3781 aggcgtggcg taaatgtcag gcctggttga aagatattga aagcgctcgg ttgcagcata
     3841 accaggcgta taccgaagcc atgcttaccg agtatgcgga ttttttccgc caggtcgagt
     3901 cttcaccgct gaatccggcg caggcccggg cagtcgttaa tggcgagcat tctctgttag
     3961 tgctggcagg tgcaggaagc ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc
     4021 tggcgcgtgg tgaagcgtcc cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg
     4081 ctgaagagat ggacgagcgt attcgcgaac ggctacatac cgaagacatt accgcacgca
     4141 cgtttcatgc gctggcgctg catattattc agcagggcag caaaaaagtt ccgatagtca
     4201 gcaaactgga aaatgatacc gctgcccgtc atgaactctt tattgctgag tggcgcaagc
     4261 aatgcagcga aaagaaagcg caggcgaagg gctggcggca atggctgacg gaagaaatgc
     4321 agtggtcagt gccagaaggt aacttctggg atgatgaaaa attacagcgt cgtcttgcat
     4381 cacgcctcga tcgttgggta agtctgatgc ggatgcacgg tggtgcacag gcagaaatga
     4441 ttgccagtgc acccgaagag attcgcgatc tgttcagtaa acgtatcaag ttgatggccc
     4501 cgttattaaa agcctggaaa ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc
     4561 ttattcatca ggcgattgtg attctggaga aaggtcgctt tatcagcccg tggaagcata
     4621 ttctggttga tgaatttcag gatatctcgc cgcagcgggc agcgttgtta gcggcattac
     4681 gcaagcaaaa cagtcagacg acgttgttcg ccgttggtga tgactggcag gcgatttacc
     4741 gattcagcgg tgcgcaaatg tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg
     4801 atcgctgtga tttagacacg acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc
     4861 ggtttattca gcagaaccca ggccagttga aaaagccgct aaacagctta accaatggag
     4921 acgaaaaagc cgtcacgtta ttggatgaga gtcaacttga cgctttgctg gataagctct
     4981 ctggttatgc caaaccggaa gagcgcattc ttatcctggc gcgttaccat cacatgaggc
     5041 ctgccagcct ggaaaaagcg gcaacacgct ggccgaagtt gcaaatcgac tttatgacca
     5101 ttcatgccag caaagggcaa caggcggatt acgtcatcat cgttggcttg caggagggga
     5161 gtgatggttt tccggctgcg gcgcgggagt cgattatgga agaggcgcta ctgccaccgg
     5221 ttgaggattt cccggacgct gaagaacggc ggttaatgta cgtggcgctg acccgggcac
     5281 gccatcgggt atgggcactg tttaacaaag agaatccctc tccctttgtg gaaatactga
     5341 aaaatctgga tgtgccggta gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt
     5401 gcaatattac ttcagacggt ccgcgagata acgctgataa tcggggatca gaatatcgac
     5461 cgcgtcgttg aaatgcggcg actggattat gaagtctgcc gttgccacgt tggtggcgac
     5521 cggaatgttc cataccgtcg ccagacgcaa caaggctttc acgtcaggat cgtgcggcac
     5581 ggcatttagt gggtcccaga agaaaatcaa tacatcaatt ttcccttctg agatcaatgc
     5641 gccaacctgc tggtcacccc ccattgggcc actcaacatc gcgttgacgt tcatgccggt
     5701 cgcgcgggaa attaagttac cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa
     5761 cggttgatgc cgttccaccc agctcatcag catttgtttg cagtgatcgt gtgccaccag
     5821 cgcaatatgt ttccgcgcag gtaaagtgcg agtcgtcagt tccataatgt acatccgtag
     5881 ttaactttcc tacagattac tgtaagcact tatcgctgca agataaagac cgaaaaagcc
     5941 tgcgcacagg cacaaaaatc tcaggaagat ggttgttttt ccgcccattg taggaaagta
     6001 tttcgcgttt gtgggtcagc cagtttaaac caatacttca gccgttgttc tgtgagcacc
     6061 tgagactgcg gtggaatagc atccagctcg gaaacacccg aaagcagcgt actgtcatct
     6121 gccatcatcg tggcaaattg cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca
     6181 acctcgacct cataatcaat cgtttttgcc gttgaggtaa tggcgagaat atccagcttt
     6241 accggaatcg gcgtcgcatc gccatccaac aattcaaggc gcggcgcggc atcaaaatgg
     6301 ttagcttccc gctcattctc caggcgaggc aggcgaaaat tgacctggtt gatgagctgg
     6361 gtattaaaac tgaccaccag cggtggggag atatacagcc gttcttcact attggaaaga
     6421 tgaatcgtct tctcaacgcg aaacactaac tgatgcggtc cattatccag ttcaatgctg
     6481 tccgcaccgc gcagcagaga gctggaaacc ttcttgccgt cgagtaccag aagatcgaca
     6541 tcggttgaaa gccgcagcgt ggtggcaaaa acggataccg gcaaacataa tgcaatcaag
     6601 gtggtcacga tgccggtttt cataggtagc tcctgtcaaa agaccgcccg aataataatg
     6661 tgtcaaaatt tttctttaat catacttact aacatataga catatttccc gcttttgctc
     6721 tcaatcattc gtattagctg catggttggc atgtcggctt tgtcgtacac tttgttaaac
     6781 agtctggagg aaataatgaa agaaaccgat attgcaggta ttttaacgtc cactcacacc
     6841 attgcgctgg tgggggcaag cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat
     6901 ctactcgacc aggggtatca cgtcattccg gtttcgccaa aagttgccgg caaaacgctg
     6961 ctgggccagc aggggtatgg cacgctggca gacgttccgg aaaaagtcga tatggtcgat
     7021 gttttccgta attcagaagc ggcgtggggc gtggcgcagg aagctatcgc cattggcgcg
     7081 aaaaccctct ggatgcaact gggggtgatt aatgagcaag cggcagtact ggcacgggat
     7141 gccgggttaa atgtggtgat ggatcgctgc ccggctattg agatccctcg cctgggcctg
     7201 gccaaataaa aaatccccgg aaggcaaaaa ccttccgggg atttgttcag ggattagtta
     7261 cgcagacgcg gggcctggag ttgtttgcgg atggtctgcg ccagctcatc catggatggc
     7321 tgttcaggat gttcatcctg caattcgcta cttagctggg cttcagccag gtaggtatga
     7381 accggtaggc cgttatcgtc ctccatcacc acgtgatacc acggagcggc gcgaagctcg
     7441 tcattcaccg ccagttcatc aggcgatggt tcagagagcg aataaaccgg gtcgatatcc
     7501 acgaccactc cgagataacc taacagggaa tggcggacct gctggccgat accgaatttg
     7561 ctggcaatca tagtcacctc ccgggaaatc tttacccttg atataagggt aaaattccac
     7621 atttcaagtt acatgacgcg acaggcaaac cctttcagat atagcccttc cgggtaggta
     7681 gcgatcaccg gatgatcggc tgcctgacgg aactgctcta taaattgtac atcacggccg
     7741 gcatcaattg cggcatccgc gatgattttc tgaaataaat cgctggtcat cagaccggaa
     7801 caggagaaag tcaggagaat accgtcttca ttcagcaact gaatcgccag catgttgata
     7861 tctttatagc cacgacacgc gcccatcaac tggcttttat tctcaacaaa cttcggcggg
     7921 tccatcacga taacgtcaaa tttttcaccg cgatcgcgat aagtacgcag caatttaaag
     7981 acatcatcac ggacaaactc agccttgctc agatccagtt tgttcagctc aacgttctgc
     8041 cgtgcaatat ccagcgcttc ctgggaggta tcaacgctga caacctggct gcaaccgccc
     8101 atcagtgccg atacggcgaa accaccggta taggagaaac agttcagcac acgtttattt
     8161 tcaacgtagc ggcgggtagc caggcggctg tctcgctggt ccaggtagta gcccgttttg
     8221 tgtccgtgct gaatatccac cagcagcttc atgccgtgtt cttcaatcgg cagcagggca
     8281 ggcggcaact cgccggtgac ggggccttgg gtcagctcca tcccttcttt tttacgtacc
     8341 gcaacatcgc tgcgatcgta aatcgcacat tccgggtaca gcgtttgcag ggcactaatt
     8401 aatgccgcgc gctgatactc tgccccagca ctcagcagtt gcagcaccag aaaattaccg
     8461 aaacgatcga tagtaatacc cggcaggcca tcagattctc cggcgattaa acgatagctg
     8521 tcgaggccat ctttttgcgc cagccagtca cgccattttt gtgcttgttg caaacggcgg
     8581 gaaaaaaaag caatgtcgat agactcagac gggtcaaacg tccagacgcg cgcccggatt
     8641 tgcgaagctg gcgaataagc gccgcgtgct aaccattttc cctgatgatc aacaatatcg
     8701 atggtttcac cgaggctggc tttaccttcc atgcgggcaa cggccccgga aaagacccac
     8761 ggatggcgac gaagtaatga tttttcgcgc cctttggcta acactaaacg tacactcata
     8821 atttactttt ctgtcgatgc aaagaaatgg gcgtcattgt ccggagtttt ccatggattt
     8881 gcaacgcgtc cattaaggat aaggagagca gcaatgtcga aagtctgcat aattgcctgg
     8941 gtttacgggc gagttcaggg cgtaggattt cgctacacca cacagtacga agcgaaaaga
     9001 ctgggtttaa ccgggtacgc caaaaatctt gacgatggca gcgtggaagt ggttgcttgc
     9061 ggtgaagaag ggcaggtaga aaaattagtg cagtggttaa agagtggcgg cccgcgttct
     9121 gcgcgtgtag aacgggtgct cagcgagccg catcatccct cgggggaatt aaccgatttt
     9181 cgtatccgtt aaatacattt taccggtttt ggcaggccag caattttggt ggcttgctta
     9241 gccggacctt tcggaaacag tcggtataaa tagcggctgt tacctttttc ttcgccaaat
     9301 ttattcgcca tcgcttttac cagcatacga atcgccggag aagtattgaa ttccagatag
     9361 aaatcacgca caaaacgcac cacttcccag tgttctggcg acagcgaaat cccttcgttc
     9421 tttgcaatca ccactgccag cggttcactc cactggctgc tttctttgag atagccttcg
     9481 gtatccgttt ctatctcttt accttcgaag atcagcataa ttattactac cttaatcaga
     9541 ctggcggcag tgtaacaaaa aacaaagccc cgcataaagc gaggctatga aagtgttagc
     9601 gggtgagatt aatcgcggct ggcgaagccc agaatgctca gcaggctgac gaagatgttg
     9661 tacagcgaaa catacaggct aaccgtggca cgaatatagt tcgtttcacc gccatgaatg
     9721 atgttgctgg tttcaaacaa aatagcgcca gaggagatca gaatgaagac cgcgctgatc
     9781 gccagatgca gagcaggcag ttgcaggaag atattagcaa ccataccaat cagtaccacc
     9841 acaatacccg ccatcagcat accgccgagg aacgacatat ctttgcgggt ggtcagcaca
     9901 tatgcagagc agcagaagaa cactaacgcc gttccgccca gtgccatagc gattacgtca
     9961 cccattccgg cagacagata ggtgttcaga attggtccga ggatataacc cagaaaaccg
    10021 gtaaaggcga atgcggagat aatcccggtc ggcttattcg ccgttttata ggtcaggaac
    10081 atcaaaccat acatacccac cagcgtcaga atcagacccg gagatggcag catcagcacc
    10141 gtactggcag ttgcggtaat cgccgaaaag gccagcgtca ggctcagcag aaaataggta
    10201 ttacgcagca ccttatgggt gctaagcagt gatgtacggt catgtgaaga actaacaata
    10261 cgatccattg agtcactctc tatgacagat gtaattaatt aagcagcata atgataatgc
    10321 gtaagggcac ccagaagttt tacccatctt tacgcatttg atctggaaca ggtttaacag
    10381 cggattatca ggtcattaag caaatataac gccctgagaa tttcgacagg caaaagaaaa
    10441 aggggttagc atttagctaa ccccttatct tatctggcgg aagcgcagag attcgaactc
    10501 tggaaccctt tcgggtcgcc ggttttcaag accgaagaaa aacaaataaa aatcagtgca
    10561 atacgtgaaa ttgctgaaaa gttaatcaaa tttaacacca agaaaaatca aggtattagg
    10621 aagttgctca tagctagtat accaagtctt tttgccagaa atttttctgg attgtctact
    10681 tgctaaccac gagcggcaaa tccagtgttg gtgtgatttt tgttttacga tcgtaaatca
    10741 acacctgatt ttctgtttta tgcccgctga aaatttgttt gtcgcgactg ctgccttcgt
    10801 aatctgagat ccccttggct tttatgtcgt gaaaattgca cccgaacgga acgccaactt
    10861 tttgctcggc tgcgcgttta gcctgattcc accagttatt cagcgtttta gctatgactt
    10921 tcccgccttt ggttgtattg atcacatatt cgcatgtaca ggaagataca tttcgggcta
    10981 actggatcgc tgtacgtaat cgcggtgacc attccttgat ttgtttggtg ccggttttgt
    11041 tttgctcaat gtagatccct ttatccataa tatcctgcca tttcaactca agcacatcac
    11101 cgagtcttgc cgcacagaga taggaaatct ccattgcaat gcgtaactgt ggaattgctt
    11161 ccgcatatat cgccgcatac tgttcatcgg tgatgtaaac agtgcgggct ttaagagaga
    11221 attttctgac tcctttgcat gggttattct tcacataccc acgctcatat ccccacccgt
    11281 atactcgact caggcttgcc agttcatgat ttgcctgggt cttgctctca agccctcgtt
    11341 tatccatgaa aattcttacc tgctcagttt tgacattatc agcaagcact tttccgaata
    11401 ccatcagcaa cgccttctga tgttgtcgat aatctttttg ggttcggggg gccagttctg
    11461 taaatgcagg ggagtccata aacatgtgcc ataatttagc tacggtcatt atgttgtgga
    11521 gttttgcttt ttccagttca taattttgcc agactttagc tacgctggtt ttccgcactc
    11581 ttcctagccc tatacttctt gtacttcctt cgggtttcca cacgtaactg taaccattcg
    11641 atctaacccg cggtggcagt acattatctt ttttattttt tcttggtctt cccattgttc
    11701 agcgcctcaa aatcgggttc agcagaaacc agttcagatg tttttggcat cgttgtcaga
    11761 ccgtgtggaa tatccctgcg gagaactatg ggttcgtttt tagggccgat tacaaatggg
    11821 atgccgtgca gtcttaactg gtgttgctgt tttgtgtatc gctcgtattt cgtgatctct
    11881 ttaatctctg ctggagatag agttaattcg tacatgtggt cacgtgcctt tacagcatga
    11941 ccgccgccac tatagctggt gggcggcgat atgggttgaa catcaaaaat cagcctgact
    12001 cgggagcagt ttttgccaga tggctgaaac gtatttcgcc tggtaacgtg catcgttcag
    12061 agcgttatgg cgttcacctt cgaatggaat agtcgttctt gcgtcgaaat ccatcaccag
    12121 tcccagagca accatcgttc ttacatcgcg atcattggtg taacgccacg ggcaggggat
    12181 cccctgccgt tcatatgaac ggcgtaaaat cacgttgtcg aaagttgcac cgttacccca
    12241 gacctgaaca aaaaattcac cggagttttc gtcgataaat tcccgtaatt gcagcagtgc
    12301 atcatctaac gggatttcat cggtaagaat ggcggattgc gcttcgcgtg actgtttcag
    12361 ccaccactta atggtgtccc gatcaatgac tccgccagca gtttccagat cgatagtttt
    12421 gctgaattct ggccccatct ctccggtttg cggatcgaaa aatattgcac ctattgagat
    12481 aatcggggca tcaggatttt ttcccatggt ttcaaggtcg atcatcagat gaatccccgc
    12541 tctgctggtg gatgtgagat tatgatgacc gttcgcctta attaagggat ctgacgcctc
    12601 gccagtttca ctatcgctgg catgatgctg attgccgcca gtgttctcct tgtgctgatg
    12661 cgcagtgcct tccatttcct ccggatcatt ttcctgaact tcaggctgat tctcttcatc
    12721 gaatatttcc tggtatgttg cgtcacttat caccgcacca caatcagggc agctaccgcc
    12781 gccggtctga ccgcaggcgg tgcaggcttt ttccggctcc tgtttcgttt ctggctcgtt
    12841 ttgttgcgca tttgggctgt tttgttccgt tttctggtcg ttctgttccg attcttgctg
    12901 gttctggttc acagaatcgc aggtttcaat ccccttcacc catttcggat cattcggatc
    12961 gctaatccct tcaacaaatt caccacgtga tgcagcaagc aatttatcgg cgtcaggctg
    13021 gctgatattg gctgcctgca taattttgtt tacttcgtca gcggtaactt ttaccggctc
    13081 cggttgtacg gaatcttcag cggtatccac attttgcggt aagtccgtgt atgttccgtt
    13141 tttgcgggca agatattctt ctttcgtgat ttcagtagcc ccggcagcca gtgccttatc
    13201 cagaccagaa agtttgtttg cgcgaccgta tttttcgcca tccttgtcgg tgaagaggaa
    13261 gtagaacggc ccctcacgct ctacagatgg ttcgacttcc actttgcatt cggttttttc
    13321 gttgtccgga attgccgttt ccactgcatc agtttctggt actggcgacg agagagtatc
    13381 agttgcgctc tgattt
//
LOCUS       DAZFWP020000133        13247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFWP020000133 DAZFWP020000000
VERSION     DAZFWP020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.133"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            230..1327
                     /gene="mltA"
                     /locus_tag="ACHK0Z_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             230..1327
                     /gene="mltA"
                     /locus_tag="ACHK0Z_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU4503714.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1404..2210
                     /gene="tcdA"
                     /locus_tag="ACHK0Z_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             1404..2210
                     /gene="tcdA"
                     /locus_tag="ACHK0Z_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU4503715.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2261..2704)
                     /gene="csdE"
                     /locus_tag="ACHK0Z_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             complement(2261..2704)
                     /gene="csdE"
                     /locus_tag="ACHK0Z_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU4503716.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2704..3909)
                     /gene="csdA"
                     /locus_tag="ACHK0Z_RS18835"
                     /old_locus_tag="pgaptmp_003767"
     CDS             complement(2704..3909)
                     /gene="csdA"
                     /locus_tag="ACHK0Z_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU4503717.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4101..4328
                     /gene="ygdI"
                     /locus_tag="ACHK0Z_RS18840"
                     /old_locus_tag="pgaptmp_003768"
     CDS             4101..4328
                     /gene="ygdI"
                     /locus_tag="ACHK0Z_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU4503718.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4679..5596
                     /gene="gcvA"
                     /locus_tag="ACHK0Z_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             4679..5596
                     /gene="gcvA"
                     /locus_tag="ACHK0Z_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU4503719.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5615..6010
                     /gene="ygdD"
                     /locus_tag="ACHK0Z_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             5615..6010
                     /gene="ygdD"
                     /locus_tag="ACHK0Z_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU4503720.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6003..7103
                     /gene="rlmM"
                     /locus_tag="ACHK0Z_RS18855"
                     /old_locus_tag="pgaptmp_003771"
     CDS             6003..7103
                     /gene="rlmM"
                     /locus_tag="ACHK0Z_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU4503721.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7147..7878)
                     /gene="fucR"
                     /locus_tag="ACHK0Z_RS18860"
                     /old_locus_tag="pgaptmp_003772"
     CDS             complement(7147..7878)
                     /gene="fucR"
                     /locus_tag="ACHK0Z_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU4503722.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7936..8358)
                     /gene="fucU"
                     /locus_tag="ACHK0Z_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             complement(7936..8358)
                     /gene="fucU"
                     /locus_tag="ACHK0Z_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU4503723.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8360..9778)
                     /gene="fucK"
                     /locus_tag="ACHK0Z_RS18870"
                     /old_locus_tag="pgaptmp_003774"
     CDS             complement(8360..9778)
                     /gene="fucK"
                     /locus_tag="ACHK0Z_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4503724.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9887..11662)
                     /gene="fucI"
                     /locus_tag="ACHK0Z_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             complement(9887..11662)
                     /gene="fucI"
                     /locus_tag="ACHK0Z_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU4503725.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11695..13011)
                     /gene="fucP"
                     /locus_tag="ACHK0Z_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             complement(11695..13011)
                     /gene="fucP"
                     /locus_tag="ACHK0Z_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4503726.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3205 a         3297 c         3484 g         3261 t
ORIGIN      
        1 accaggctgc tccaccccgc gtccgtggat gcgcactata ctccgctcgc gttttcatgc
       61 aacctttttt ttaccccgat cacggattta gcatgatttt gaacaaaaac aaaccactaa
      121 agcctatttt ttgtacaaaa tttgcgtcag ggagaggctg ctcatttcat taaacgcggc
      181 ggtttgttat cttcgttgcg ccttattttt taacctgaag aagagaacaa tgaaaggacg
      241 ttgggtaaag taccttctta tgggcacggt tgtggcaatg cttgccgcct gctcttccaa
      301 accaaccgat cgcggacagc aatataaaga cgggaaattt acccagcctt tctctctggt
      361 gaaccagcca gatgccgttg gcgcgccgat taacgccggt gattttgccg agcaaattaa
      421 ccatatccgt aattcgtcac cgcgtctgta tggcaaccag agtaatgttt ataacgcggt
      481 gcaagagtgg ctgcgcgcag gcggtgatac ccgcaatatg cgccagttcg gcattgatgc
      541 ctggcagatg gaaggtgccg acaactatgg taacgtgcag tttacaggct attacacgcc
      601 ggtaattcag gcgcgccata cccgccaggg cgagttccag tatcctattt accgtatgcc
      661 gccaaaacgt ggtcgtctgc cgtctcgtgc ggagatctac gcaggggcgc tgagtgataa
      721 atatattctc gcttacagca actccctgat ggataacttc attatggatg tgcagggtag
      781 cgggtatatc gactttggtg atggcagtcc gcttaacttt ttcagctatg cagggaaaaa
      841 cggtcatgcc tatcgcagca ttggtaaggt gctgatcgac cgtggcgaag tgaaaaaaga
      901 agatatgtcg atgcaggcga ttcgtcattg gggcgaaaca cacagtgaag ccgaggttcg
      961 cgagctgctg gaacagaacc cgtctttcgt tttctttaaa ccgcaatctt ttgcaccggt
     1021 gaaaggggca agtgcggtgc cgctggttgg ccgtgcttct gttgcctccg atcgttcaat
     1081 tattccgcca ggtactacct tgctggcaga agtgccgctg ctggataata acggcaaatt
     1141 taacggtcag tacgaattgc gtctgatggt ggcgctggat gtcggtggcg caatcaaagg
     1201 ccaacacttc gatatctatc aagggatcgg gccggaagcc ggacatcgcg caggttggta
     1261 caaccactat ggacgtgtct gggtgctgaa aaccgccccg ggcgcaggta acgtctttag
     1321 cggctgatgt gttattctgt ataccgtttt tacggatacg ccagggtgag ggataacctc
     1381 acccttttta attctgaggt gctatgtctg tggtaattag tgatgcatgg cgtcagcgtt
     1441 ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca actgtttgct gacgcgcata
     1501 tttgtgtggt tggtatcggt ggtgtcggtt cctgggcggc ggaagcgctg gcgcgcacgg
     1561 ggattggcgc aatcacgctt atcgatatgg atgatgtgtg cgtcaccaat accaatcggc
     1621 aaattcacgc tctgcgcgat aacgtcgggc tggcaaaagc ggaagtcatg gcggagcgta
     1681 ttcgccagat taacccggag tgccgtgtaa cggtggtgga tgatttcgtg acgccggata
     1741 acgtagcgca gtatatgagc gtgggttatt cgtacgtgat tgatgccatt gatagtgtac
     1801 ggcccaaagc ggcgctgatt gcttattgtc ggcgcaataa aatcccgctg gtcacgaccg
     1861 gtggcgcggg tgggcagatt gacccgacgc agattcaggt taccgatctg gcgaaaacga
     1921 ttcaggatcc gctggcggcg aagttgcgcg agcgcctgaa aagcgatttt ggcgtagtga
     1981 aaaacagtaa aggtaagctc ggcgtcgatt gcgtgttttc tactgaagcg ctggtgtacc
     2041 cgcagtcaga cggtactgtg tgtgcgatga aagccacggc agaagggccg aagcggatgg
     2101 attgtgcatc tggatttggc gcggcaacga tggtgaccgc cacctttggg tttgttgcgg
     2161 tttctcatgc gctgaagaag atgatggcga aagcggcgcg tcagggttaa acaatcaggt
     2221 cggatgcgac gcgaacgtct tatccgacct ggttctgtgc ttaaacctgc ttcgccgcag
     2281 cgataatcgc ctcgcttaac gcattcaacc cctggctgcg tgaggcgcta agctgtgcac
     2341 gcaatcccag ctcatcaaac aatgccagcg gcgactgtgc ctgcaattcg gcgacggttt
     2401 tcccctcaac ggcagtcaac aacaccgcca acaggccgcg cacaatgcgc ccttcgctgt
     2461 cgccaaagaa gtgcatttta ccgttttcag tcacggtata tcccagccag acgcggtttt
     2521 cgcatccggc aatctcttta gcctgcgctt ttaactcgtc tggcaaagcc ggaagctgtt
     2581 tccccagcat gatcaactgg cgatatttat cttcccattg cgacaacggt gcgaaggtat
     2641 tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg tccggcgaat tgcgggtttg
     2701 tcattaatcc accaataatt ccagcgcgcg gtcaacggca ttcaccagcg catccacatc
     2761 actctttgta ttatatggcg caaaagaggc gcgcagtgtg ccggttacgc ctaattctgc
     2821 cagtagcggc tgagcgcaat gctgcccggc ccgcagggca ataccgtact ccgccagcag
     2881 cgtcaccata tcgctatgat gaacgccagc aaaatcaaag gccagcaggc tggaatcctg
     2941 gcagcggaat gaacgaaagc cgggacgttt cgccagcgca tcttccgcca gcgttgctaa
     3001 gctacggctc cagctttcgg cctggttgat atcgtaatct gccagccatt ccagcgccgc
     3061 gcttaatcct atgacaccag cgacatttgg cgttccagct tccagtttcc acggcgcaga
     3121 ttgagtcgtg aagccgtcaa aactcacttc gtgaatcatt ttgccgccac ccagccaggg
     3181 cgacatcgct tccagcagtt ctgatttgcc atacagcacg ccgatacccg tcgggccata
     3241 cagtttgtga ccagagaaag catagaaatc gatatctagt tgctgaacat ctgcggggaa
     3301 atgcaccgcc ccctgagcac catcaaccat caccaccatc ccggctgaat gagcaaaggt
     3361 aatcgctcgc gccagatccg ggcaaccgcc agtaacgttc gacatctgac ccaacgccag
     3421 aatccgacta cggggagtaa tcagttctgg caacaaatcg acatccggca gtcgctgcgc
     3481 attaagcggc aatttcacca ctttggctcc agtttgttgg gcgaccatca gccaggggac
     3541 gaggttagcg tggtgttctg ccacgctgac aataatctca tcgcccggtt gcagacgcgg
     3601 acgcgcatag cattgtgcca ccatgttgat ggattcagtg gtgccgcgcg tccagacgat
     3661 agttttatca tccggtgcat tcagtaattg cgccactttc tctcgtgcag cttcataacg
     3721 cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg acgtttccgg cgctcaggct
     3781 gtaaaactgt cgggtggctt caaccacggc ttcaggttta agcgcggtcg cggcgctgtc
     3841 gagatagacg cccgcatcct gtagagcggg aaactgggcg cgaaactgcg cgggattaaa
     3901 aacgttcatg gtactcctcg gcttgatgca ccgatcgtga cgcaaaattc actttgatac
     3961 aaggagagtg ctaaccatca gaattatctg gatatcctgg cgaagccgcg taagtaacat
     4021 atgctgaata gtgttaggcg ttaattttag cctgttaatc aatactatta agatttatta
     4081 attgctttaa ggagaagatg atgaaaaaga ctgccgcaat tatttctgcc tgtatgctga
     4141 cttttgccct gagcgcctgt tccggttcga actatgtgat gcacaccaat gacggacgta
     4201 ccatcgtctc tgacggcaaa ccacagactg ataacgatac cggtatgatt tcgtataaag
     4261 acgctaatgg caacaaacag cagatcaacc gtactgacgt gaaagagatg gtcgaactgg
     4321 atcagtaatt cacgatcgca aggtaaaaaa aagcaccgca attaggcggt gctacattaa
     4381 tcactatgga cagacagggt aaatgtacag gaagtgaaaa aaggtagctt tgctaccatg
     4441 gtctgaatcg cagaccaatt gcaaacacaa caacacaaca tcacaaccgt aagccaaaag
     4501 ttcaccagaa cacgcattcc gataaaactt ttcgttccgg ctcaggaagt gccgccacta
     4561 taggtatttg ctggtagaag ctcaacggac aatttataat ggctcagatt aaaaaaacta
     4621 ataggttaca cagtgtgatc taattgttaa attcatttaa catcaaagtt taaaagccat
     4681 gtctaaacga ttaccaccgc taaatgcctt acgagttttt gatgccgcag cacgccattt
     4741 aagtttcact cgcgcagcag aagagctttt tgtgacccag gccgcagtaa gtcatcaaat
     4801 caagtctctt gaggattttt tggggctaaa actgttccgc cgccgtaatc gttcactcct
     4861 gctgaccgag gaagggcaaa gctatttcct cgatatcaaa gagatatttt cgcaattaac
     4921 cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg gcgttgacgg tcagtttact
     4981 ccccagtttc gccattcatt ggttggttcc gcgactttcc agctttaatt cagcttatcc
     5041 gggaattgac gttcgaatcc aggcggttga tcgtcaggaa gataagctgg cggatgatgt
     5101 tgatgtggcg atattttatg gtcggggcaa ctggccgggg ctacgggtgg aaaaactgta
     5161 cgccgaatat ttattgccgg tgtgttcgcc gctactgctg acaggcgaaa aacccttgaa
     5221 gaccccggaa gatctggcta aacatacgtt attacatgat gcgtcacgcc gtgactggca
     5281 gacatatacc cgacagttgg ggttaaatca tatcaacgtt cagcaagggc caatttttag
     5341 tcatagcgcc atggtgctgc aagcggctat tcacgggcag ggagtggcgc tggcaaataa
     5401 cgtgatggcg caatctgaaa ttgaagccgg acgtcttgtt tgcccgttta atgatgttct
     5461 ggtcagtaaa aacgcttttt atctggtttg tcatgacagc caggcagaac tgggtaaaat
     5521 agccgccttt cgccaatgga tcctggcgaa agccgctgct gaacaagaaa aattccgctt
     5581 tcgttatgaa caataattta cgtagggtac gaccatgacc agccgtttta tgctgatttt
     5641 cgccgccatt agcggcttca tttttgtggc tctgggcgct tttggcgcgc atgtgttaag
     5701 taaaaccatg ggggccgttg agatgggctg gatccagacc ggcctcgaat accaggcgtt
     5761 tcatacgctg gcgatcttag gtctggcggt ggcaatgcag cgtcgcatca gtatctggtt
     5821 ttactggagt agcgttttcc tcgcgttagg cacggtgctg ttcagcggca gcctttattg
     5881 cctggcactg tcccatctgc gtttgtgggc gtttgtcact ccggttggcg gcgtgagctt
     5941 ccttgcgggc tgggcgttaa tgttagttgg tgctatccgt ttaaagcgca agggcgtaag
     6001 tcatgaataa ggttgtattg ctgtgccgtc cgggctttga aaaagagtgc gccgcagaaa
     6061 ttaccgataa agccggccag cgggaaattt tcggttttgc ccgcgtgaaa gagaatgcgg
     6121 gttatgtcat ttatgaatgt tatcaacctg atgatggcga taagttaatc cgtgagctgc
     6181 cgttcagttc attaattttt gcccgccagt ggtttgtggt gggggaactc ctgcagcatt
     6241 tgccgccaga agatcgtatt acccccattg tcggcatgtt acagggcgta gtagagaagg
     6301 gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag caaagagttg ctgaaattct
     6361 gccgtaaatt caccgtgccg ctacgcgctg ccttgcgcga tgccggggtg ctggcgaact
     6421 atgaaacgcc gaagcgtccg gttgtgcatg tattcttcat tgcaccaggt tgctgctata
     6481 ccggttactc atacagcaac aataattcgc cgttctatat gggcattccg cgcctgaaat
     6541 ttccggcaga tgcgccgagt cgttccacac tcaaactgga agaggcattt catgtgttta
     6601 ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat gtgggcggtg gatttgggcg
     6661 cttgcccagg cggctggacc taccaactgg tgaagcgcaa catgtgggtt tattccgtcg
     6721 acaacggccc gatggcgcaa agtctgatgg ataccgggca ggtgacgtgg ctgcgggaag
     6781 acggtttcaa attccgtccg acacgcagca atatctcctg gatggtatgc gatatggttg
     6841 aaaaaccggc taaagttgcg gcattgatgg cgcagtggct ggttaatggc tggtgccgtg
     6901 aaaccatttt caatctcaaa ctgccgatga aaaaacgcta cgaagaagta tcacacaatc
     6961 tggcgtatat tcaggcacag cttgatgaac atggcataaa tgctcagatt caggcgcggc
     7021 agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg ccgcatctgg gctgcggtgg
     7081 gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg tcgtctccgg cctgctaccc
     7141 tttccgtcag gctgttacca aagaagttgc aacctggcgc tcatcagaaa taatgtgcgt
     7201 tacctcatcc agatgcccga tgcgggcttc ccctgaacga ttaaatttac ttttatcaat
     7261 caataacaac gattgcgcgg cacgttttaa tagcatcgat ttgtaatcag cgttgatggc
     7321 attggagtcc cacagtgcgc cgctgctatc gatcccttca caagaaaaaa taaacagatc
     7381 gatttccagc gatttaagtt gggaaatcag cgagggattg acgtaacagc catatttgcg
     7441 ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg cgtttaccga gttcatggca
     7501 aatcggatgg ctattggtga agacctgaat gttgatgtca ggcaactggc gtgccagata
     7561 ccagcaagtt gaactggcgt ctaaggctat caccatccct tcttcaatcc acgcgagcgc
     7621 ctcgcgcgcg atatctgctt tatgcgcata atggcttttc agccggatgt gaaagggatc
     7681 gccactgtct tgattttgac ggtggatata tttagcgcgt ccatgattgc gcagaatttt
     7741 accctgcgtc tgtaattcat tgagatcgcg acgaatggtt tctttactga cctttagctg
     7801 ttcagagaga gcttccgtgg tcaggctggt atggttcagc agcaggtcga ctatcgcttg
     7861 ctggcgtgcc gctttcatat ttttcacccc atcattcgat aaccgcccgc gtcatgcggg
     7921 cgtaccggta tgagattacg gtgttacccc tttttttaaa agaatattcc cgtacttcgc
     7981 tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc cgttcataaa acgcaaaacg
     8041 attgatgcgg atgatgtcag gacacggggc ttgtagtgaa agcgcattac ggtaacgtcg
     8101 ttctacttca ggatcgagag tgtcaccttc taccgctgcc atcatcacca gcggcggggc
     8161 ataactgtcc agctcaaata acgggataat cgcctggagc aagtcgctca ccaacaggcc
     8221 atcagcgcgg atcacctgcg gtcccatcga atgggcggga aagtgagcat cggaaaaaat
     8281 aatttcatct ccatgtccca tctctgccag cactttcaat agttcgggag aaattaacgg
     8341 cgaaattgtt ttcagcattt cacacttcct ctataaattc aggttcagtt tgcgggtaga
     8401 aataacggta ctgataatga atctgtgcac gggcttcttc cgggctgttg aattccccta
     8461 cgccatacca accgaacagc gcagctcctg cgacggtcgt ttcggcatcg tcgagaactt
     8521 ttaccggaat atcaagcata ttggctttaa tctgattcca caatgtgttg cgacttcctc
     8581 caccgactaa caataattca gaggccttaa agtgcccgat tttttccagc atctgtagat
     8641 tgcgctgtaa ttgcgcagtt aacccttcca gcgccgcgcg atagaaatgc ccccgcgtgg
     8701 tattaagcgt cactccttgc cagccagcgt tctgacacga caataaatca cactgcattt
     8761 ttacgccatc cgcgccaggc gcgatcagac gagcttcttc aatcaacatt tgccagggtg
     8821 tttcagccgt ccagaacagt tttctcaccc attccagcac gccggatgcc agccattgca
     8881 tacctgggtt atacaaccct gcctggctat ccagttcgca ggtggaaccg gcgtactgac
     8941 ttaacagcga agtatcaacc tgggcgctac ggaccattaa aatttcccat gtaccggaag
     9001 agagcacggg ttcattttgg tcggcaccag cgccaaaaag ggcgaactgg gtatcgtgac
     9061 ctgcggaaat caccggtatg ccaacgggta agccgagcat tgctgcggcg ctgttctgta
     9121 gcgtaccaat ctgttcaccc gcttccacca gacgagggaa gagtcggcgt ggaataccgg
     9181 tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat atccagcatc tggctggttc
     9241 cggccatcgt gatatcagta gtgaattcgc cggttaaacg gtggttaatc agcgacgaaa
     9301 taaagagcca ggcgtgcgcg cgttccagca gttgtggatg attttctttc aaccacacca
     9361 acttatataa cgtattgaaa ctaaaggctc cgacgccaga aatagcctgc aaccgctgtg
     9421 cggagatcaa ctgttcaata ttgtccataa ccgctgctgt tcgcggacat ttccagctaa
     9481 tgatcggata gagcagattg ccttgcttat ctaccagagc gccatccaca ccaaaggtgg
     9541 tgacggcgat accgcggatg tggcattcag tcagttcact attgatttgc cgacagcaat
     9601 cagcaaagcg ttgcaaaatg gcgtctaaag accactggtg ccaggtgttg ttttccatcg
     9661 cgatatcgct ggcattaggc gttgaggcgc gggcaacaat ttttccctgc cgattaaccg
     9721 cgatggccct gacattggtc gcgccacagt cgagtaccag gataacttct tgtttcataa
     9781 tcgctcctgc aatatagccg gataacattg cttatccggc taaccactct tgcttgttca
     9841 ggcccggtag ccggagcgac cgggcatcac atcagggagt aatgtattaa cgcttgtaca
     9901 acgggccgta gttctggcaa gcgcggtaat cctggccttc aatatccatg ccgtgcgcag
     9961 cccaggcaga aggacgatac actttggtct cttcaacgtt gtgcatacat accgggatac
    10021 gcagcatgga ggcgagagtg ataaagtctg cgccaacgtg gccgatggtc agaaccccat
    10081 ggttagcgcc ccagttcgcc attaccgagt acacatccgt aaacggccct ttaccggtga
    10141 ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt gcgtttgttg aggatgtcat
    10201 gcacatcctt cggcaattcc acgctccagc cttccgcgat ttgcagtacc ggtcccaggc
    10261 ctttgatgat gttgacacgg gtcatggtga acgggacgcc gccttcggta aggaagcggg
    10321 aagagtaacc gccgccacgg aagtattcgt ggatcgccgg gcaccattcg gtagcggcga
    10381 ggcaagcgtc agcctcttgc tgagagattt cccagtgcgg cttcatcgtc gggttacctt
    10441 cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga accggagttg atcaaatgga
    10501 tgatgccgtg ttctgccagt ccatcgagtt tatgccccgt tacacgctca attgcttctg
    10561 gtgaccagta ggtacgcaca tcggcaaata cctgagcggt gccggtgagc tggtgaccca
    10621 ttagcattgc cacgccgtta agactgtcgt tttcggtcgc cacgacaaag ggttcgcgca
    10681 cgccattcca gtcaaatgaa ctgttaagga tcgcttcggc ggtgtcacca ttgggatatt
    10741 gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat ggcgttgtag ccaagtgatt
    10801 cttccacgcg accaatatcg gccagtttgc tgttgccttg catcatgtcg cggatacaca
    10861 tcgccatcag taaactttcg cgcagaactg cgcggctttg ctcggcattg cgttgatact
    10921 gtttgttatt ttcatcttcg ccatagcgga agtttttatc agcccaggtc agtgccattt
    10981 ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg acgcagttcg gtcatatcca
    11041 ccgcctggac tttcattccc agccaggatt caaagaagtt gtgatcaaca atagaaccgg
    11101 cgatacccat cgaaacgccg cccagcgaca gatagctttt acctttcatg ctggcgacgg
    11161 ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac atcggcagga atcgatgtgt
    11221 catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc cgggatgcct ttctggctgt
    11281 gagctgccag agccgctgcc aggtaaacag cgccgggacg ttcagtgccg ttaaagcccc
    11341 aaatggcttt cgggcgggtt ggatccatgt cgatggtttc actgccatag caccagcaag
    11401 gcgttaccgt aatggtgagg cctacattct gactgctgaa tttttcttcg caagcagcgg
    11461 cttcagccat acccgcgata caggtatcgg aaatgacaca ctcgacggca gctccgcagg
    11521 catggcgcag tttctcggtc agcagtgcgg ccgtagcttt cgccatattc attgtttgtt
    11581 cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat aaccgggcgg ataccaattt
    11641 tcggtaagct gatttttttc attacagatt cctcacttta ttcggaaaat atgttcagtt
    11701 agttgccgtt tgagagcgga aacgggcaaa gataaagatg accgcgaagc agagtgcggg
    11761 gatcagttca gcagtgggga tgttgcccgc cgcgtcactg acaaaaccca tgaccggagt
    11821 gacaataccg ccgccaatga tggtcataac gatgaaggac gaaccgtatt tggtgtcctg
    11881 gccgagattc ttaatgccca gcgagaagat tgttgggtac tgaatcgaca taaaggcgct
    11941 gcataaagtc agggctatta agcccacatg accgccagcg aaggctgaga tcaggcacag
    12001 tgccatagcg attaatgcgt aggcggccag gactttgtgt ggtgcgaagc gactgatgag
    12061 ccaggtaccg gtgaaacgac caataaagaa gcacaccatg gttccggtta aatagttagc
    12121 ggcaaagcct gcagtcatac ctggaatttc ttctacagcg tagcgaatca aatagctcca
    12181 gcaggccgtt tgtgcgccaa cgtagcagaa ttgcgctaat accgcccagc gccagtgacg
    12241 aatacgcgcc agacgagaaa gcgatgcgga gaacgatcct tgtttggcgt cactgtgatt
    12301 atcactctgc aatgccggga atttcgtcag catgatcagc agggcgacca gtaacacgat
    12361 agccacgatg atcatataag gtgtctgtac cgataatacc aggctgtgtt tatacgcact
    12421 caattgctct ggagacattt tatcgagaac gtcttgcgat tgatgtggca cgttagacaa
    12481 aataagactt tgcccaaaga caaccgcgat aattgcgcca aacgagttaa atgtttgcgc
    12541 aagatttaag cggaagtgac cactgctttc cggccctaat accgtaacaa aagggtttgc
    12601 ggcagtttcc agacaaccta atccggctgc aataataaat aggccaacta aaaataaggt
    12661 gtagttcatt atttctgcag cgggccagaa taatgcagcg cccaaggcat ataaaaataa
    12721 tccggtaata atccctgctt tataactgag ttttttcatc aatatcccag cagggattgg
    12781 gataatgaaa taaccaaagt aaaaggccga ttggatcagg ccagcctgga aatttgtcag
    12841 cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta aggttattgg ctaccgccca
    12901 aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg taacttctgc tttgccctgc
    12961 atctttatct accgcacggt aactctgcgt ttgtattgat gtgtttccca tagcatcctc
    13021 ttaggttcag aagcttaagc acgtaatcgg ttatgttttc aaaagtgcaa aaccagcgag
    13081 ttcgggcttc atatcagtga atatttgcct gtctgaaatg aactcatgga ttcattttca
    13141 aataaaaaac gctctgtaat atgacggcgg tcacacttat tagctggaag gtgactttat
    13201 gtgactacca tcactttaat aatcgaaatt tcatgcccgt ttcagaa
//
LOCUS       DAZFWP020000134        13172 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFWP020000134 DAZFWP020000000
VERSION     DAZFWP020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.134"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(43..1500)
                     /gene="dtpC"
                     /locus_tag="ACHK0Z_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             complement(43..1500)
                     /gene="dtpC"
                     /locus_tag="ACHK0Z_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU4503727.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(1559..3706)
                     /gene="cadA"
                     /locus_tag="ACHK0Z_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             complement(1559..3706)
                     /gene="cadA"
                     /locus_tag="ACHK0Z_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU4503728.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(3786..5120)
                     /gene="cadB"
                     /locus_tag="ACHK0Z_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             complement(3786..5120)
                     /gene="cadB"
                     /locus_tag="ACHK0Z_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU4503729.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(5486..7024)
                     /gene="cadC"
                     /locus_tag="ACHK0Z_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             complement(5486..7024)
                     /gene="cadC"
                     /locus_tag="ACHK0Z_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU4503730.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(7284..7398)
                     /locus_tag="ACHK0Z_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /pseudo
     CDS             complement(7284..7398)
                     /locus_tag="ACHK0Z_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(7773..8345)
                     /locus_tag="ACHK0Z_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             complement(7773..8345)
                     /locus_tag="ACHK0Z_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4503731.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     GSAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(8442..8639)
                     /locus_tag="ACHK0Z_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             complement(8442..8639)
                     /locus_tag="ACHK0Z_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4503732.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(8651..9139)
                     /locus_tag="ACHK0Z_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             complement(8651..9139)
                     /locus_tag="ACHK0Z_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4503733.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTRDVGESEWIHFT
                     GTGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDANAECLPGLPTF
                     DW"
     gene            complement(9136..9513)
                     /locus_tag="ACHK0Z_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             complement(9136..9513)
                     /locus_tag="ACHK0Z_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4503734.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            complement(9603..9971)
                     /locus_tag="ACHK0Z_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             complement(9603..9971)
                     /locus_tag="ACHK0Z_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4503735.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(10134..10355)
                     /locus_tag="ACHK0Z_RS18935"
                     /old_locus_tag="pgaptmp_003787"
     CDS             complement(10134..10355)
                     /locus_tag="ACHK0Z_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4503736.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10418..10894)
                     /locus_tag="ACHK0Z_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             complement(10418..10894)
                     /locus_tag="ACHK0Z_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4503737.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(10910..11395)
                     /locus_tag="ACHK0Z_RS18945"
                     /old_locus_tag="pgaptmp_003789"
     CDS             complement(10910..11395)
                     /locus_tag="ACHK0Z_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4503738.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFIAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTEYYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(11450..12268)
                     /locus_tag="ACHK0Z_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             complement(11450..12268)
                     /locus_tag="ACHK0Z_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4503739.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12368..12601)
                     /locus_tag="ACHK0Z_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             complement(12368..12601)
                     /locus_tag="ACHK0Z_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4503740.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(12680..13135)
                     /locus_tag="ACHK0Z_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             complement(12680..13135)
                     /locus_tag="ACHK0Z_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGU4503741.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
BASE COUNT         3453 a         3143 c         3259 g         3317 t
ORIGIN      
        1 actgaaagtt aatttattca taaccaattg tttttacgac agttaatcgt tgctctcctg
       61 tatcatattc gtcggcgtgc taaacagaaa acgggtagca aaggccaata cgacgatgat
      121 cgcgacacaa gccaacgtcc actctcccat ctgagaaaag aatcgctggt aagctgcaat
      181 tgccataccg ctaatttgcg actctgtcgt ctgctgtgcc acgacgcctg ccagccagtt
      241 ggcgaccgcg cctgttgcca gcatataaat accggttaat acgccagaca ttttcagacg
      301 cgtgatttgc gcaatcgcca ccgggtcaat aaagagttcg gcaaagccca ttagcgccaa
      361 cccggatatc atcacgccca ttgacgcttg accgtcagcc gctgcatgtc gggcatcaaa
      421 tgccaacaac ataaagccac aagccatcag cagtaagcca aaggcaaact tcagccagac
      481 gcgcaatgtt gagttgccgc ggctttctgg cgacgccagc caggccagta caaccccagc
      541 gagcatcacc gcaattgcat tcaccgactg gaatagtgct gtaggtactt caatattgaa
      601 tgcctgacga ttcacaaagc gatcgataaa caagctgatg gtactgccgc cctgttgtgc
      661 cagtacccag aacaatgtcc cgacaaacat caacaataca atttgccaaa gagcacggcg
      721 gtgttcgggg aatttgacca tcatgcgggc aatgatttgt gcggcaatga ggcaaacgat
      781 cgccagcaaa tatccggacc agtcgttctc cagcagcagg gtaaaaaata ctggggctaa
      841 acagagcatc accactaacc agctccatac cggtaaggca aatttgacgc tagtgagcgc
      901 ttttttatcc atactacgcg tggactggaa atgacgatgc ccgcttaaga aaatcaacaa
      961 accgataaac atgccgccac ccgcaagggc aaagccaaca tgccatccat accactgagc
     1021 agccaggccg caggcgattg gggcggcgat agaaccgata ttgcccgcag catacagcag
     1081 cgaaaaaccg ccatcacgtc gatgatcgtt ctcgtcgtaa agctcgccaa gcaaacagct
     1141 gatgtttgat ttgaataaac cgtagccaca aataatgatt gccagcgcca gatacaggct
     1201 aaaggttgaa tttgtatcaa tgcccagcac cacatggcca agggtcatta acagcgcgcc
     1261 ggcaatcact gcagtgcggt tgccgagcag gcggtcggca agccagccgc cgagaatagg
     1321 ggtaacgtaa accagagaag catatgcgct gaacaggctg atggcatggt tatcatcaaa
     1381 accaagctga tgggtgagat agagaatgag taaggcacgc atgccgtaaa aactgaagta
     1441 ctcccagatt tggatcgcca cgatatagta tatcgcgcgc ggctgtgagg gtgttttcat
     1501 gtgttctcct tatgagcaaa aaagggaagt ggcaagccac ttcccttgta cgagctaatt
     1561 attttttgct ttcttctttc aataccttaa cggtatagcg gccatcagcc tgacggtatg
     1621 caccgtgaat atcggtttca aagcccggat agtgagcgcc gatttcacac agcatctgca
     1681 ggaactccag aaccggacgg ctttcttcgg tgatcatttc acccggcatt accagaggaa
     1741 ctcccggcgg atatggaagg atcatattgg cgttaatacg acctaccatt tcgtcgaggt
     1801 aaacttcttc ggtcataccg tgcagctctt tctggaacgc agcatacgga gtcattacca
     1861 tcgtcggcag cacttcaaat gcgcgataca tcagatccgg cagattgtgg tgaacaatca
     1921 gtttgtggat attctgagcc agttcctgaa tacgcatgtt ttcatagaat tcaggatctt
     1981 cacgatacag agacggcagc atgtttttca cacgcaggtt caggtcgaac gcacgtttga
     2041 agtcagtcag agcacgcagc aggctcagtg ctttggtctt atcgataccg atgctgaaca
     2101 ggaacagcag gttatacgga ccggttttct caacaacgat gccatgttcg tcgaggtatt
     2161 tcgccacgat gctggccgga ataccaaagt cgctcatggt gccgtctttt tccatccccg
     2221 gagtcagcag ggtgactttg atcgggtcaa gatacatgtg ctcgttatcg atgtttttga
     2281 agccgtgcca ggtgctgtca gaacgcagcg gccagcattc agtggtatcg atatgatccg
     2341 gctgccatac atcaaagaac cagccatcag attccgttct cagacgtttg atctctttac
     2401 ggaatttgat cgcacgttca atggaaccgt tgatcagacg cttaccagca ttgcctttca
     2461 tcatcgccgc tgcggtttca gtggacgcca cgataccgta gtgcggagaa gtggtggtgt
     2521 gcatcatgta ggcttcgtta aaggtttctt cgtttacgtc acctttaacg tggatcatgg
     2581 aagcctgaga gaacgccgcc agcagtttgt gagtggactg ggtttcgtaa atcactttcc
     2641 cttctacacg gccaccgctc ataccgcatt taccttcgta aatcggtgag aagttggtgt
     2701 aaggcaccca cgcggagtca aagtggatgg atttcacatc cagtgttttc ttgataaagt
     2761 cggtgttgta cagcagacca tcataggtag agttggtaat tacagcatgt accggccagg
     2821 ttgcgtttgg tgtttctttc acgcgcttag caatggtagc gtgctggaat tcactctgtg
     2881 ggataccacc aagaataccg taagcgttac gggtcgggcg gaaatagatt ggcgtaacat
     2941 cgctcatcat catcaggtgg gtcagcgatt tgtggcagtt acggtcaatc agaatggtgc
     3001 tgcctgctgg agcagagtac ataccaacaa ttttgttcgc agtggaagta ccgttggtca
     3061 ccatgtagct gcggtctgcg ttaaagacgc gagcgatata ctgttctgct tctttgtgtg
     3121 gaccactgtg atccagcaga gaacccagtt cagatactga aatggaaata tcagatttca
     3181 tggtgttcgg accaaagaaa tcatagaaca ggctacctac cgggcttttc tggaatgcag
     3241 taccgcccat gtgaccagga gtacagaaag tatatttacc ttcacgaaca tatttaaaca
     3301 gtgctttagt cagcggaggc agaatagtgt tgatatattc gtcagtggtc tgcttgatct
     3361 tattagcaat atcttcagca gcacccagcg catattcaaa gaagctaatc tgtaaacgca
     3421 ggtcattcag gcttacatcg agagtggaat acgtattagc gaacgcgtac aacggcaggt
     3481 tctcgttcat tttgctaatt tcttcgcaca gctcgagatt atatttatcc cagtcaaaaa
     3541 taacgccgca cagacgcgca ttgttttcga tcagttttaa taagtcgtca cggtcgttcg
     3601 ggtaaacaat ctggaagttc agacgttcaa gcgcgcgatg aagttcacgg atgggttctt
     3661 ctttaaaata aacccccatg tgattcaata ttgcaataac gttcatagtc atatctccag
     3721 gtaaaaaagg cccctcccaa cacatgggac aaaatgaaag gaggagcctc ggaaaatact
     3781 tttaattaat gtgcgttaga cgcggtgtgg ttatccattg agtggctctg gcgctcgtgc
     3841 attttgcgag cgtagaacat caggataatc aggctgacga tgaaggtacc tgccagctcg
     3901 aaggagcttg cgcccatcag cgcgatgaag cagaacacac aacccagtac agagcagatc
     3961 aggctgacaa agttgcggat gttaacgcct tcaaaacgaa tcaggtcaac gcaagagtag
     4021 aagtacggca gcatagtcag cagtactgcg ataccggtca gttcaccgaa caggtcagat
     4081 gctttaccac cggcagagtt catcagagtg atgaggatca tcagggcagt cattttcact
     4141 gcagccagca gcagaccttt tttcggaata ccgttgctgt cgacttcacc ataaactttc
     4201 gggaagttac cgtcgttagc ggcacgtaca cctgcctggc ctaccaacat catccaggag
     4261 cccagagaag tcaggcacgc aaaggcggtg aatgcagaaa ccagcggcgc agcccagtta
     4321 ccgaggatgg ttgaagcact gattgcaaac ggagcaccgg aagctgccat tacagaagat
     4381 ggatacatac cggaaagcac ctgagtcgca gcgatgtaaa caatacctgc taaaccagta
     4441 cccagcatgg ttgccagcgg aacggtacgt ttcgggtttt taaccatacc agtacttact
     4501 gctgcggatt caacacccac gaaggcccac aggcagagca gaatactttt aatgatcgca
     4561 tgaccatcag tggtatccgc agtattccag ttagctgcat aagttgccgc atcaaaccaa
     4621 tgccagccaa caatagcggt cattactacc ggaataagaa ccagcaccag accaatagtg
     4681 gttaaacggc ttacccaagt accgccgagc atatttacaa aggtaaatac ccagacgata
     4741 gcaatacagg cgatacccgc cggaacagga tcatttaata ctgggaagaa ggtggaaaga
     4801 taagataccg cggtaatacc aatcgccagg ttaccaatcc agttagcatg gtaataaaga
     4861 acacctgtct gaaaaccaaa tgcaggggaa atttctccgg cataagcaat tgggccacct
     4921 tgttgcgggt tttttgttgc cagtcgggca tatacatacg ccagcgacat tgcaccaata
     4981 atagagataa tccaacccca gatagcaata ccaccgatac ttgctaggtt cgcaggtaat
     5041 aatgcaatac cgctccccat catattaccg gcaacaacac cggtacaggc aaatagcccg
     5101 atcttcttgg cagaactcat gctcttctcc taatttcatt tttgaatttg gagtccgggt
     5161 catgatgtat aactatttcc tgaccagacc aaactggcgc taagaatact cacgaaaaaa
     5221 ggattaatcc taaagattag gtgaaataac acaaaagttt ctgtaagtga gaacttgagg
     5281 ttttttatta acacatcagg atcgcaagtt gatatcatga aaagataaac attcaatgtt
     5341 tacaatggat tgcgggatat tctctggtta aatttatgtg ataaaaatta tgcggcaaat
     5401 aaattgccgc aacatattat accaatagaa acatacaaaa actcaacaac aaatattccc
     5461 gagcataaat caacccggga gttacttatt ctgaagcgag aaatttgtcg agataaggta
     5521 caacataagg aacagaagtc tggaatatac cattttcaat ccagtaaagg gtgtttgccc
     5581 ctgggcgtaa attaaaggcg gtgagatatg catcagccgc ttcccggttc atccccttca
     5641 tttcataaac cttgccaagc aacacataat ttagccagga catttcaaga tcaatgccag
     5701 tatttatcgc ctggtaagac tcatctgttt taccttttac cagagcactg accgctttta
     5761 tttgatatat aatggacagg ttattcaatt ccggcagtgt aacaatgttg tctatttctg
     5821 tgttcagtgc tgctaattgt ttttcatcta aaggatgttg agaatggcgc acgatatcaa
     5881 ctaatgcttt ttctgctctc gcgtaggtaa attctgggga tgattgaaca atctcaccta
     5941 ataattcact ggcacgattc aatgatttat catcgccatg cagtaaataa tcatgtgcct
     6001 gataaaaatt agttaataac gcaccacgat gaggcaatat ttgctggagc gtctcctgca
     6061 ttcgttgtgg ccacggttgg tttaacgctt ttgataaact ctccagtaaa tcattttgaa
     6121 tcgccagctg attaccgtta gtgatgacat aacgtttatc cagcatggtt gaaccatctg
     6181 cattgtctac caattttatc gacataaagc attgttgagc acggtattgg cgctgattaa
     6241 caaacgcaat agataatgtt ttaccggaac tgctcggttc atcaatgttg tagttgattt
     6301 tgtcatgcac cataaaggtg gagaaggtgt taagtgatgt cgccaccaaa tcacccacgc
     6361 ctatcgcgta agagagctga tacggggaac tccagctgtt acaactttta tttaccatat
     6421 taatgtcaat atcgcgtgga ttgagcaaaa tacgcgattt gctcatagga agacgtgtat
     6481 caagagttga aaacgctacc agtgctacac agatacctaa cgacaacagg aaaaaaaacc
     6541 atacccaaaa ggtagtgaat cgtttgcttt taactgggga ttgttcaggt ggcgttgcgg
     6601 tgttttgaat gttaagactg tgggagggag aatctgtggc aggaaccgcc tctggtatag
     6661 ggggaggcga agatagcatt atttcctctc cctcttcttc gctgtaccag ataaccggca
     6721 ccattaattt atagccgcgc tttggtacag tagcgatata gacaggacta tcttcatcat
     6781 tatcttttaa tgacttacgt agttctgaga tactctgcgt cacaacatga ttggtgacaa
     6841 tacttctctt ccagacatta tcgataagtt catccctgct aagtacttcg ccactgtgtt
     6901 gagcaaagaa aaccagaaga tcgattaatc tcggctcaag ggtaagttga cgcccattgc
     6961 ggctaatttg gtttatggac ggagtaacaa gccattcgcc aacgcgaact acaggttgtt
     7021 gcataataga aactcattcg aaaagggaat gatgcaatga taattgccac aacctatttt
     7081 taccatctat agatgggttt atttacatat tattggtgaa tgcaagacgt tatttttacc
     7141 agccataaac ttcttgatta catagtatta cgaaaggatt ttactgagaa ccagaagtaa
     7201 tattccttac catcaaaatt catcatcttt gccaaagaaa aatgttcaga aaataatcca
     7261 tggaaaattg tccggagcgc ttactatttt aatggattgt tagtctttgc atgagcaagc
     7321 ggactgatac atttctcttt gttctcattc agaaaatctc atcagtcggc gttcagaccg
     7381 gtgttgtgct ttatggatgc gttacgctcc tgatgacgtc atttgacgtt caacaacatc
     7441 acggggccgc acgacatttc acgtcagtta gtgctatcgc tcaggaacaa attttcccga
     7501 attgggatat gcccgcaaat tgctggtgat gtggggagaa tctggttgag ttcgggagaa
     7561 ttgatttgga ggcagaacgc ttaaattgtg gcgtccagaa acgaaaaacg gacctccgtg
     7621 gaggtccgtt gatatgaatt tggtgccgga ctcggaatcg aagaatgctt ctatcatgtt
     7681 gatattattt tattttgttt ggtttggtga aatatgtacc ctcttttgta cccgcagtcc
     7741 cttttgatat cgaaagggaa tcaaactcgg ggtcaggctg agagcacaga aggaaagtgc
     7801 ctggctatta tatcgttaat tctgtcaatc agctccagcc ttttgaaagt gatgtgtgca
     7861 gatccaccct tcttaaagta tttaatgatg aacatctcat cttcatagcg tcctttaccc
     7921 tgaacggact gtatgtggtc atccagatta atggtgatat tccctcggtt gtcggggatc
     7981 ggtttaccgc tgagcagatg caacattctt tccaggtcag ccaggcgatc tgcctgcatt
     8041 ccactgataa gatgaaatcc ccactggtcc catctcacca gattgttgac gataatttta
     8101 ctgccaaatt tacagggtga gttggttttg taatcccagc tcagcccttt gaagacgttg
     8161 atcactcccc gttcaaacac ttcatctttg ttctggtgca gttgttcaaa tgtgctcaga
     8221 atgttcgctt cgctgatttc cggaaaatta tcatattcca gtgacctgga cctgactgag
     8281 gtggagcaga taacgtccgt attctcgttg accacttccg gacgggagac tgtgatttca
     8341 gacataatcg atattccttt ttgcagataa atgaaaacgc cagccgtgtt caggctggcg
     8401 tcagtggaat gaagtctggt gagtgagatg atgagtgaga ctcaccggtt ctggtgctga
     8461 agctgcagtt ggcgcagggt acggatatcc tctctggcct gttcgataca gggcaacagt
     8521 gctgctgaat ccgttttatc ctcattgttg tcaaagataa ttcccgattc gcagtcgata
     8581 ttgtcctgca gccacgcaat cagaatatcc agcgctgttt ccgtggttaa tgatttcatg
     8641 ttgtttctgg ttaccagtcg aaagtgggta aacccggcag gcattcagcg ttggcatcca
     8701 gatgaatgag actgacgcca taacgtcggg taagtgtgac aaccagacga cggaacgttt
     8761 ttgacaggcc caggcgctta agccgcagaa ccgggtacga ccacgcatca gtacgtaaca
     8821 gataaccggt acctgtaaaa tgaatccatt ctgattcacc aacgtccctg gtctggtgtg
     8881 acagcgagta caaccaggcg ttgtccgttt ccgtgatatg tgcggtactg cagcgtatgc
     8941 cggcaagggt tgcaaatggc ggaggtgtgc aggttgactg ttggtcggat tcctccacca
     9001 cacggagtga ataaccgttt tcagcgacgt tattaatcag ttcagtgaga ttaacaccat
     9061 cgacgtcaac gacaatgcgc cccatgttca gtgcctgcac gttaacgctg tcagcttcca
     9121 gcgtcagggc cagtttcatc gtttcgcctc ctgatactta cccagggtaa tgttatttac
     9181 cgttcggtaa ttgtcgcggg tcatcaggcc agtcgcccgg cgagcccgga ggatatcgat
     9241 gctgtttatt aactgagagc gggtacaggc actgaattcc ggctggtcgg tacgcaccag
     9301 tgcgtatttt tcaacaagaa agttcactgc atcgcacagt gaaatgccag cttcaatatg
     9361 ctgctcaatc acacgttcat cggcgaacgg tgtgtcattc agtgtaaggc cgtaatgctg
     9421 gcccagtagt cgggagagca gagtctgcca gatggtgacg ggagacgggc agctcgatgc
     9481 ctcccgtacg tgtgtatcgg gtaatgtttt catactgaag gttttcctga tatgcagaga
     9541 tgaaaaatgg aaaagtggcg tggtgaaagc accaggccgg agcagaaggt tattccggtg
     9601 gtttactttt tcgtttcggg cgtcggataa acagccagat aaacgtaacc accgctaccg
     9661 agagtgtcag ctttgcaggt cagacctttt gcatacagcg tgacggtatg ctgatggcgg
     9721 ggattcagtt caccgctggt gagcatgagt tccagttgtt tcatcagcag cggaaaggcc
     9781 tggtccaggt ggtacgcatc tgcatcgctg aaccggcctc tgataccggc acggtcggca
     9841 aggtaatgca accggttacc ctcctgcacc agacgggcgc cgaaacaggg cgtcactgtg
     9901 cagggtagtc cccaccaggg atggtcgtca ttgtcatcgg gatgcgttgt cccggaaagt
     9961 gtgtctgaca cgataaaatc ctcacagaaa atcggtgaat gatagtttaa cgatgacggg
    10021 tcagccggcg cgcagaccgg cttcggtaaa ggtgtattcg ttgatgagca ggacctcatc
    10081 cacagcggcg tctgagctca gccagtcgta ctcgttttcc gggtgacgga acatcagttc
    10141 agggtgacgc tcatcagccg gacatacgga ccaaaactgt ccttacggcg ttctgcaaac
    10201 acggccagca caccgggaat atcctgcact tcacgaccgg tatacgcttc agcactgccg
    10261 tgccagcggt atttgccggt acagaacgga aaaagacggg atgccggatg ctgacggtga
    10321 atacgcatgg cttcgccacg ggtgatgatt ttcatagtgg gatacctctg aagacagaag
    10381 ataaaagtga aaacaggtgt gatgtggttg tgacgggtta aagcagacca tgttcggcaa
    10441 aggagaaaac ctggctgcca ccgactatca gatggtccgg cacccggata tccaccaggc
    10501 ccagtgcctg taccagacgt tccgtgataa ggcggtctgc cttgctgggt gtgacttcac
    10561 cggacgggtg attgtgtgcc agcaccacag cagcggcatt gtgatacagg gcgcgtttaa
    10621 tcacttcacg gggatggact tccgtgcggt tgatggtgcc ggtgaagagg gtttcaccgg
    10681 caatcagctg attctggttg ttcagataca gcacccggaa ctcttcacgc tccagtcccg
    10741 ccatgttcag aatcagccat tcccgtgccg cacgggtgga ggtgaaggcc acaccgggtt
    10801 catgaagatg gcggtccagg gttttcagag cccgcagaat gagactgcgc tcgccgggcg
    10861 tcatttctcc gggcagaaag gaaagctgct gcattgtccc tgcctccatt cagtcgatga
    10921 tgcgcataat ggcgctgcat tccggatgct gcagggcgta atcccgcagc cggtaataat
    10981 ggaccgtcat ggcataatac tccgtacgac aggcgtgatg actgtacgtc atcagacagg
    11041 cagcaatgcc ggcggcttcc gggctcattt cagcgcggtt accgttcatg gcattgaaca
    11101 gtacccatgt ttcgccatca tcgtcatccg gttcgggcgc tataaatgcg ccaccgttgt
    11161 tcagtgtgta cagattccag ataccaccgc agtagtcttc gcaaagacgg tccatccagc
    11221 cgaagatacg gggctccagg gtcacccact gtggaatgag gccaaaatgc tgcggccaga
    11281 agctgatgcg ctgttcatca gggactatgg tggcaaccag ctgaggctgg tcattccctg
    11341 atgcagcggt tacggaaaca gaagatgtgg tggaattatg tgaaatggtt ttcatgaaat
    11401 gactccttaa aggacaaata aatgcgtggt tttcagggta atggctgaat cagcgcaggc
    11461 tctccagcag ggtttccgcc atcacccaca atgcgcggtt gagcttaatg tcagtgtcaa
    11521 tgctgtgaat ggcacgggta tggatacgtt ttcctttagc gctgcgacct gaaattccgc
    11581 ctttcagcat attctcctga atggtctgat aagcactcca caggtcctta ccgtaatcct
    11641 cccggcgtcg tggcgtcaga atgtcggcgg tggtgacggg ctgatgttcg tcaccataac
    11701 ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg tggcggcaga atcagcgact
    11761 gcatggcatc acgcttttcc tcaatccggt caaaaacccc caccacctcg taagcccctt
    11821 caatgactct gtccaccaca tttccccggt gcggaacacg cacttccccc agagactgac
    11881 cacagacgca tccgttctgg cagacgaacc tgaagtaacc cggcagcatc tggtagctgg
    11941 aggtaccgtc atgagagttg agcagaataa tttcagggac atgttctccg tttatctctc
    12001 cggcccgccg cagacgcagc atgtgttttg tgtatccccg gcggcccggg tcgcgcacac
    12061 gggtctggca ggcgaagaat ggctgaaagc cttcccgctg caggctttcc agtacggtga
    12121 tggtggggat gtacgtatag ttctggcttc gggacgtgtg tttatcttcc ccgaaaatcc
    12181 ccggcacata gtgcatcagt tcttcgtgtg tcagcggacg gtcacggcgt atctggttcg
    12241 cataaccaaa acgactggct agtcgcataa tttgctcctt atcggtggtt aagatttact
    12301 ggtgtaataa atgaaaaagc cacgtctccc ggagaagacg cggcctgatg ggtgaaatga
    12361 atgacgttta ttgtctgaga agaccgtcac tggcgagccg cgtattaagc acctttccgg
    12421 catcgggttc agaattccgg cagcgccagc gtcgctgacc ttccgcgttg cggataaccc
    12481 gacggaaatg cattcccggg tcatcctgga taaacacatt gcggtaagct gttatgacag
    12541 caaccgcctg cccgtatgag aaagatcctt cagccaggac atactctgtg taacccggca
    12601 tatctgtttt ctcctgataa ataaaaagcc tctgccacgc gttgtgacag aggctgaagc
    12661 atgaaactga cttcagggat cagttaacgt tttttccgga aacggtaatc agcagtggat
    12721 ggtagtcctg gggattgaaa accgataacg gcagactgac acgatggccg ttactttctt
    12781 cagttgcttt aatgatttcg gttgtggcga cattttccac gcactccgtt tccagaaatg
    12841 cgtctgtggt tcgcgtggca ttactgtcac caaaagcttc cgtttccatt tttctggtca
    12901 ccagcgtctg accatatttg tctttgagtt gcagagtgat ggtgaggggg ccaaatcctt
    12961 catcgtttcc gccattatcc agccggaact ggtaagcaca aatatttccc gggagccata
    13021 tcgtatctgt attgcgtata ctgatgtaac gttgatcctg tgcccggagt ggggcagacc
    13081 acgttaaccc cagaatgaag gcggtaatca tgcaggtttt gaacaggtga atcatggtat
    13141 ttacctctct gagtcatgac gatgacactg ac
//
LOCUS       DAZFWP020000135        12996 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFWP020000135 DAZFWP020000000
VERSION     DAZFWP020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.135"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            223..1332
                     /gene="livK"
                     /locus_tag="ACHK0Z_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             223..1332
                     /gene="livK"
                     /locus_tag="ACHK0Z_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU4503742.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1380..2306
                     /gene="livH"
                     /locus_tag="ACHK0Z_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             1380..2306
                     /gene="livH"
                     /locus_tag="ACHK0Z_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU4503743.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2303..3580
                     /gene="livM"
                     /locus_tag="ACHK0Z_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             2303..3580
                     /gene="livM"
                     /locus_tag="ACHK0Z_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU4503744.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3577..4344
                     /gene="livG"
                     /locus_tag="ACHK0Z_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     CDS             3577..4344
                     /gene="livG"
                     /locus_tag="ACHK0Z_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU4503745.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4346..5059
                     /gene="livF"
                     /locus_tag="ACHK0Z_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             4346..5059
                     /gene="livF"
                     /locus_tag="ACHK0Z_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU4503746.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5455..6771
                     /gene="ugpB"
                     /locus_tag="ACHK0Z_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             5455..6771
                     /gene="ugpB"
                     /locus_tag="ACHK0Z_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU4503747.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6869..7756
                     /gene="ugpA"
                     /locus_tag="ACHK0Z_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             6869..7756
                     /gene="ugpA"
                     /locus_tag="ACHK0Z_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU4503748.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7753..8598
                     /gene="ugpE"
                     /locus_tag="ACHK0Z_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             7753..8598
                     /gene="ugpE"
                     /locus_tag="ACHK0Z_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU4503749.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8600..9670
                     /gene="ugpC"
                     /locus_tag="ACHK0Z_RS19005"
                     /old_locus_tag="pgaptmp_003801"
     CDS             8600..9670
                     /gene="ugpC"
                     /locus_tag="ACHK0Z_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU4503750.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKCRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9667..10410
                     /gene="ugpQ"
                     /locus_tag="ACHK0Z_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             9667..10410
                     /gene="ugpQ"
                     /locus_tag="ACHK0Z_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4503751.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10397..10837)
                     /locus_tag="ACHK0Z_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             complement(10397..10837)
                     /locus_tag="ACHK0Z_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU4503752.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10957..12699
                     /gene="ggt"
                     /locus_tag="ACHK0Z_RS19020"
                     /old_locus_tag="pgaptmp_003804"
     CDS             10957..12699
                     /gene="ggt"
                     /locus_tag="ACHK0Z_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU4503753.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAINFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12737..>12996)
                     /locus_tag="ACHK0Z_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     CDS             complement(12737..>12996)
                     /locus_tag="ACHK0Z_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HGU4503754.1"
                     /translation="KANHYLDDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAARL
                     AGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2872 a         3333 c         3734 g         3057 t
ORIGIN      
        1 tgctgacaaa ggcacttttt tctgtttatc tatcaataaa ttcagaatat tatctgttct
       61 taatcgactg aaaaataggg attttaatca ctattatcac aaaatactgc gctaaaccct
      121 taatcagaca ggcaaaaaca gtgcagtata aaaaaagaac agtctgattt gttaacacat
      181 aaaaacaaag caacacaaca tcacgaatgg ggatttttga ctatgaaacg gaatgcgaaa
      241 actatcatcg cagggatgat tgcactggca atttcacaca ccgctatggc tgacgatatt
      301 aaagttgccg ttgtcggcgc gatgtccggc ccgattgccc agtggggcga tatggaattt
      361 aacggcgcgc gtcaggcgat taaagacatt aatgccaaag ggggaattaa aggcgataag
      421 ctggtgggcg tggaatatga cgacgcctgc gacccgaaac aagccgttgc ggtcgccaac
      481 aaaatcgtta acgacggcat taaatacgtt attggtcatc tgtgttcttc ttctacccaa
      541 cctgcgtcag atatctacga agacgaaggt attctgatga tctcgccggg agcgaccaac
      601 ccggagctga cccaacgcgg ttatcaacac attatgcgta ctgccgggct ggactcttcc
      661 caggggccta cggcggcaaa atacattctt gagacggtga agccccagcg catcgccatc
      721 attcacgaca aacaacagta tggcgaaggg ctggcgcgtt cggtgcagga cgggctaaaa
      781 gcggctaacg ccaacgtcgt cttcttcgac ggtattaccg ccggggagaa agatttctcc
      841 gcgctgatcg cccgcctgaa aaaagaaaac atcgacttcg tttactacgg cggttactac
      901 ccggaaatgg ggcagatgct gcgccaggcc cgttccgttg gcctgaaaac tcagtttatg
      961 gggccggaag gtgtgggtaa cgcatcattg tcgaatattg ccggtgatgc tgccgaaggc
     1021 atgttggtca ctatgccaaa acgctatgac caggatccgg caaaccaggg catcgttgat
     1081 gcgctgaaag cagacaagaa agatccgtcc gggccttatg tctggatcac ctacgcggcg
     1141 gtgcaatctc tggcgactgc ccttgagcgt actggcagcg atgagccgct ggcgctggtg
     1201 aaagatttaa aagctaacgg tgcaaacacc gtgattgggc cgctgaactg ggatgaaaaa
     1261 ggcgatctta agggatttga ttttggtgtc ttccagtggc acgccgacgg ttcatccacg
     1321 gcagccaagt gatcatccca ccgcccgtaa aatgcgggcg ggtttagaaa ggttacctta
     1381 tgtctgagca gtttttgtat ttcttgcagc agatgtttaa cggcgtcacg ctgggcagta
     1441 cctacgcgct gatagccatc ggctacacca tggtttacgg cattatcggc atgatcaact
     1501 tcgcccacgg cgaggtttat atgattggca gctacgtctc atttatgatc atcgccgcgc
     1561 tgatgatgat gggcattgat accggctggc tgctggtggc ggcgggattc gtcggcgcaa
     1621 tcgtcattgc cagcgcctac ggctggagta tcgaacgggt agcctatcgc ccggtgcgta
     1681 actctaagcg cctgattgcg ctcatctcgg caatcggcat gtccatcttc ctgcaaaact
     1741 acgttagcct gaccgaaggt tcgcgtgacg tggcgctgcc cagtctgttt aacgggcagt
     1801 gggtggtggg ccatagcgaa aacttctctg cctctattac taccatgcag gcggtgattt
     1861 ggattgttac cttcctcgcc atgctggcgc tgacgatttt cattcgctat tcccgtatgg
     1921 ggcgcgcctg tcgtgcctgc gcggaagatc tgaaaatggc gagcctgctt ggcatcaaca
     1981 ccgaccgggt gattgcgctg acctttgtga ttggcgcggc gatggcggcg gtggcgggtg
     2041 tgctgctcgg tcagttctac ggtgtgatta acccctacat cggctttatg gccgggatga
     2101 aagcctttac cgcggcggtg ctcggcggga ttggcagcat tccgggggcg atgattggcg
     2161 ggctgatcct ggggattgcg gaggcgctct cttctgccta tctgagtacg gaatataaag
     2221 atgtggtctc atttgccctg ctgatcctgg tactgctggt gatgccgacc ggtattctgg
     2281 ggcgcccgga ggtagagaaa gtatgaaacc gatgcatatt gcaatggcgc tgctctctgc
     2341 cgcgatgttc tttatcctcg ctggcgtctt tatgggcgta cagctggagc tggacggcac
     2401 caaactggtg gtcgacacgg cttcggatgt ccgttggcag tgggtgttta tcggcacggc
     2461 ggtggtcttt ttcttccaga ttttgcgacc tgctttccag aaagggttga aaagcgtttc
     2521 cgggccgaag tttattctgc ccgcgattga tggctccacg gtgaagcaga aactgttcct
     2581 cgtggcgctg ttggtgcttg cggtggcgtg gccgtttatg gtttcacgcg ggacggtgga
     2641 tatcgccacc ctgaccatga tctacattat cctcggcctc gggctgaacg tggtggtagg
     2701 gctttccggc ctgctggtgc tggggtacgg cggtttttac gccatcggcg cttacacttt
     2761 tgcgctgctc aatcactatt acggcttggg cttctggacc tgtctgccga ttgccgggtt
     2821 aatggcagcg gcggcgggct tcctgctcgg ctttccggtg ctgcgtctgc gtggtgatta
     2881 tctggcgatc gttaccctcg gcttcggcga aattgtgcgc atattgctgc tcaataacac
     2941 cgaaattacc ggcggcccga acggaatcag tcagatcccg aaaccgactt tcttcggcct
     3001 tgagttcagc cgtaccgccc gtgaaggcgg ttgggacacg ttcagtaatt tctttggcct
     3061 gaaatacgat ccctccgatc gcgtcatctt cctgtatctg gtggcgttgc tgctggtggt
     3121 gctaagcctg tttgtcatta accgcctgct gcggatgccg ctggggcgtg cgtgggaagc
     3181 gttgcgtgaa gatgagatcg cctgccgttc gctgggctta agcccgcgtc gtatcaagct
     3241 gaccgctttt accatcagtg ccgcgtttgc cggttttgcc ggaacgctgt ttgctgcgcg
     3301 ccagggcttt gtcagcccgg aatccttcac ctttgctgaa tcggcgtttg tactggcgat
     3361 agtggtgctc ggcggtatgg gatcgcagtt tgcggtgatt ctggcggcaa ttttgctggt
     3421 ggtgtcgcgc gagttgatgc gtgatttcaa cgaatacagc atgttaatgc tcggtggttt
     3481 gatggtgctg atgatgatct ggcgtccgca gggcttgctg cccatgacgc gcccgcaact
     3541 gaagctgaaa aacggcgcag cgaaaggaga gcaggcatga gtcagccatt attatctgtg
     3601 aacggcctga tgatgcgctt cggcggcctg ctggcggtga acaacgtcaa tcttgaactg
     3661 tacccgcagg agatcgtctc gttaatcggc cctaacggtg ccggaaaaac cacggttttt
     3721 aactgcctga ccggtttcta caaacccacc ggcggcacca ttttactgcg cgatcagcat
     3781 cttgaaggtt taccggggca gcaaattgcc cgcatgggcg tggtgcgcac cttccagcat
     3841 gtgcgtctgt tccgtgaaat gacggtaatt gaaaacctgc tggtggcgca gcatcagcaa
     3901 ctgaaaaccg ggctgttctc tggcctgttg aaaacgcctt ctttccgtcg cgcccagagc
     3961 gaagcgctcg accgcgccgc cacctggctt gagcgcattg gtttgctgga acacgccaac
     4021 cgtcaggcga gtaacctggc ctatggtgac caacgccgtc tggagattgc ccgctgcatg
     4081 gtgacgcagc cggagatttt aatgctcgac gaaccggcgg caggtcttaa cccaaaagaa
     4141 accaaagagc tggatgagct gattgccgaa ctgcgcaatc atcacaacac cactatcttg
     4201 ttgattgaac acgatatgaa gctggtgatg gggatttcag accgaattta tgtggtcaat
     4261 caggggacgc cactggcaaa cggtacgccg gagcaaatcc gtaataaccc ggacgtgatc
     4321 cgtgcctatt taggtgaggc ataagatgga aaaagtcatg ttgtcctttg acaaagtcag
     4381 cgcccactac ggcaaaatcc aggcgctgca tgaggtgagc ctgcatatca atcagggcga
     4441 gattgtcacc ctcattggcg cgaacggggc ggggaaaacc accttgctcg gcacgttatg
     4501 cggcgatccg cgcgctacca gcgggcgaat tgtgtttgat gataaagaca ttaccgactg
     4561 gcagacggcg aaaatcatgc gcgaagcggt agcgattgtc ccggaagggc gtcgcgtctt
     4621 ctcgcggatg acggtggaag agaacctggc gatgggcggc ttttttgccg aacgcgacca
     4681 gttccaggag cgcataaagt gggtgtatga actgtttcca cgtctgcatg agcgccgtgt
     4741 tcagcgggca ggcaccatgt ccggcggtga gcaacaaatg ctggcgattg gtcgcgcgct
     4801 gatgagcaac ccgcgtttgc tgctacttga tgagccatcg ctcggtcttg cgccgattat
     4861 catccagcaa attttcgaca ccatcgagca gctgcgcgag caggggatga ctatcttcct
     4921 cgtcgagcag aacgccaacc aggcgctaaa gctggcggat cgcggctacg tgctggaaaa
     4981 cggccatgtg gtgctctccg ataccggcga cgcgctgctg gcgaacgaag cagtgagaag
     5041 tgcgtattta ggcgggtaat aacacgttga ttgataagga gtcaaaagac tcctttgaga
     5101 caggtgacaa atgtaaaatt gcctgatgcg ctgcgcttat caggcctact gggtgagttg
     5161 caatatattg aatttgcacg atcttgtagg ccggataagg cgtttacgcc gcatccggca
     5221 tgaaacgatg agtaatctgt agagtttgat tcagaccttc tattttccgc ttatccgtgc
     5281 cccatctccc attttccctc acccacgccg tcaccgcctt gtcatctttc tgacacctta
     5341 ctatcttaca aatgtaacaa aaaagttatt tttctgtaat tcgagcatgt catgttaccc
     5401 cgcgagcata aaacgcgtga attcgcgcat tcggtacaac aagagagata aacgatgaaa
     5461 ccgttacatt atacagcttc agcactggcg ctcggactgg cgttaatggg gaatgcacag
     5521 gcagtgacga ccattccgtt ctggcattct atggaagggg aactgggtaa agaggtggat
     5581 tctctggccc aacgttttaa cgccgaaaat ccggattaca aaattgtacc gacctataaa
     5641 ggcaactacg aacagaattt aagcgcgggg attgccgcat ttcgtaccgg caacgctccg
     5701 gctattttgc aggtttatga agttggcacc gccaccatga tggcatcgaa agccattaaa
     5761 ccggtatatg acgtgtttaa agaggcgggg attcaattcg atgagtcgca gtttgtgccg
     5821 acggtttccg gttactactc cgacagcaaa acgggccact tactctccca gccattcaac
     5881 agctcgaccc ccgttctcta ttacaacaaa gacgccttca agaaagcagg attagacccg
     5941 gaacaaccgc cgaaaacctg gcaggatctg gcggactatg ccgcgaaact gaaagcctcc
     6001 ggtatgaagt gcggctacgc cagcggctgg cagggctgga tccaactgga aaactttagc
     6061 gcctggaacg gtctgccgtt tgccagcaaa aacaacggct ttgacggcac ggacgcggtg
     6121 ctggagttca acaagccgga gcaggtgaaa cacatcgcca tgctcgagga gatgaacaag
     6181 aagggcgact tcagctacgt cggtcgtaag gatgaatcca ccgagaagtt ctataacggt
     6241 gattgcgcga tgaccaccgc ctcttccggt tctctcgcta atattcgtga gtacgccaaa
     6301 tttaactatg gcgtaggcat gatgccttac gacgccgatg cgaaagatgc accgcaaaac
     6361 gccattatcg gcggggccag cctgtgggtg atgcagggca aaaataaaga aacgtatacc
     6421 ggcgtggcga agttcctcga cttcctcgcg aagccagaaa acgctgccga gtggcatcag
     6481 aaaaccggtt atctgccaat caccaaagca gcgtatgacc tgacccgtga gcagggcttt
     6541 tacgagaaaa acccaggggc ggataccgcg acgcgtcaga tgctgaataa gccgccgttg
     6601 ccgttcacca aagggctgcg tctgggcaac atgccgcaga tccgcgtgat tgtggatgaa
     6661 gagctggaga gcgtgtggac cggtaagaag acaccacagc aggcactgga taccgccgtt
     6721 gagcgtggaa atcagttgct gcgccgcttt gagaaatcga cgaagtctta atcagtgtaa
     6781 tgtcggatgc gtttcgctta tctgacctgg catcgcgtgt aggccggata agcgaagcgc
     6841 atccggcaca gttcaggaat taaccgtaat gtcatcatcc cgtccggtgt tccgctcgcg
     6901 ctggctgcct tatctgctgg tcgcgccgca gctcatcatc accgttatct tttttatctg
     6961 gcctgcgggc gaagcgttgt ggtactcgct acaaagcgtc gatccgtttg gtttctccag
     7021 ccagtttgtc ggcctggata acttcgtcac gctgttccat gacagctact atctcgactc
     7081 cttctggacg acgataaaat tcagcacctt tgtcaccgtc agcggtttgc tggtatcgct
     7141 gttctttgcg gcgctggtgg agtacatcgt gcgcggcagc cgtttctatc aaaccttaat
     7201 gttgctgcct tacgccgtgg ctcccgccgt tgccgccgta ttgtggatct tcctgtttaa
     7261 ccccggtcgc gggctgatca cccattttct cgcggagttc ggctacgact ggaaccacgc
     7321 gcaaaacagc ggtcaggcaa tgtttctggt ggtgtttgcc tcagtatgga agcaaatcag
     7381 ctacaacttc ctgttcttct atgccgcgct gcaatccatt ccccgttcgt tgatcgaagc
     7441 cgcagccatc gacggtgcag ggccgattcg ccgcttcttt aagattgcgc tgccgcttat
     7501 cgccccggtg agtttcttcc tgctggtagt gaacctggtg tatgccttct tcgacacctt
     7561 cccggtgatc gacgccgcca cgtccggcgg gccggttcag gccaccacga cgctgattta
     7621 taagatctac cgcgaaggtt ttaccggact ggatctggct tcgtctgccg cacagtcggt
     7681 ggtgttgatg ttcctcgtca tcgtgctgac ggtggtgcag ttccgctatg ttgaaagcaa
     7741 ggtgcgttac caatgattga gaaccgtccg tggctgacga tattcagcca taccatgctg
     7801 atcctcggga tcgcggtgat cctcttcccg ctgtacgtgg cgtttgtcgc ggcgacgctg
     7861 gataaacagg ccgtctatgc cgcgccgatg acgctcatcc ccggcacaca tctgctggaa
     7921 aacatccaca acatctgggt gaacggggta ggcacgaata gcgcgccgtt ctggcggatg
     7981 ttgcttaaca gctttgtgat ggcgttcagc attacgctcg gcaaaattac cgtctcgatg
     8041 ctctcggcat ttgccattgt ctggtttcgt tttccgctac gtaacctctt cttctggatg
     8101 atttttatca ccctgatgct gccggttgaa gtacgtatct tcccgacggt ggaagtcatc
     8161 gccaacctgc agatgctcga cagctacgcc ggtttaacgc tgccgctgat ggcctcggcg
     8221 accgctactt tcctgttccg ccagttcttt atgacgctgc cggatgagct ggtggaagcc
     8281 gcgcggatcg acggcgcatc gccaatgcgc ttcttttgcg acatcgtttt tccgctctcc
     8341 aaaactaatc tggcggcgct gtttgtgatc acctttatct acggctggaa tcagtatttg
     8401 tggccgttgt tgattattac cgatgtggat ctcggcacca ccgtggcagg gatcaaaggg
     8461 atgatcgcta caggcgaagg caccacggaa tggaactcag tgatggcggc gatgttgtta
     8521 acgcttatcc ctccggtggt gattgtttta gtgatgcagc gtgccttcgt gcgcggcctg
     8581 gtcgatagtg agaaataaga tggcaggact gaaattacag gcagtaacca aaagctggga
     8641 tggtaaaacc caggtcatta aaccgctgac ccttgatgtg gcggatggcg aatttatcgt
     8701 gatggtcggg ccgtctggct gcgggaaatc gacgctgctg cgcatggttg ccgggctgga
     8761 gcgggtgaca gaaggcgata tctggatcaa cgaccagcgc gtgactgaaa tggagccaaa
     8821 agatcgcggg attgcgatgg tgttccagaa ctacgcgctt tatccgcata tgagtgtcga
     8881 agaaaacatg gcgtgggggc tgaaaattcg cggcatgggc aagcagcaaa ttgccgagcg
     8941 cgttaaagaa gcggcgcgca ttctggagct ggacggtctg ctcaaatgtc gcccgcgcga
     9001 gctttccggc ggtcagcgcc agcgtgtggc gatgggccgc gcgattgtgc gcgatccggc
     9061 ggtgttcctg tttgatgagc cgctctctaa cctcgatgcc aagctgcgcg tgcagatgcg
     9121 tcttgaactg caacagttgc accgtcgcct gaaaacgact tcactctacg ttactcacga
     9181 tcaggttgaa gcgatgacgc tcgcccagcg agtaatggtg atgaacggcg gcgttgccga
     9241 acagattggc acaccagttg aagtctacga aaagcccgcc agcctgtttg tagcgagttt
     9301 tatcggcagt ccggcgatga acctgctgac aggccgcgtg aataccgaag gcacgcattt
     9361 cgaactggac ggcggtatcg tgctaccgct aaacggtggc taccgtcagt atgccgggcg
     9421 taaaatgact ctcggcattc gcccggaaca tattgcgcta agctcgcaag cagaaggtgg
     9481 cgtaccgctg gtgatggata cgctggagat cctcggcgca gataacctgg cgcacggacg
     9541 ctggggcgag cagaagctgg tggtgcgact ggcgcatcag gagcgcccga cggcaggcag
     9601 cacgctgtgg ctgcatctgg cggaaaatca gctgcatctt tttgatggtg aaacaggaca
     9661 acgagtatga gtaactggcc ttatccccgc atcgtcgctc atcgtggcgg cggtaagctg
     9721 gcccccgaaa acaccctggc ggcaatcgac gttggggcaa aatacggtca taagatgatc
     9781 gaatttgacg cgaagttatc gaaagatggc gagatcttcc tgctccatga cgacaatctc
     9841 gaacgcacca gcaacggctg gggcgtcgcg ggtgaactga actggcagga tttactgcgc
     9901 gtggatgcgg gcagttggta cagcaaagcg tttaaaggtg agccgctgcc gttgctttcg
     9961 caggtggcgg aacgctgtcg cgaacacggg atgatggcga atatcgaaat caaacccacc
    10021 actggcactg gaccattaac gggcaaaatg gtggcgctgg cggcacgcca actgtgggcc
    10081 ggtatgacgc cgccgctgct gtcatcgttt gagattgatg ctttagaagc tgcacagctg
    10141 gcggcaccgg aactgccgcg tggtttattg cttgatgagt ggcgcgacga ctggcgcgaa
    10201 ctgaccgcgc ggctgggctg cgtctctatt catctcaatc ataagttact cgataaagtg
    10261 cgagtgatgc agttgaaaga cgccggactg cggattctgg tttataccgt caataaaccc
    10321 cagcgcgcgg cagagttgct gcgctggggc gtggattgca tctgtaccga tgcgattgac
    10381 gtgattggtc cgaactttac ggcccaatag ttttcaacgg aatgtcaggc tgcggcgtgc
    10441 tgggctgatt cagcatgtcg ccgtttctcc tctccggcag catatgctgc tgattcaacg
    10501 aactatccgg attacggttg ctgtttaaca tcccgccgtt ggtgttgggc agcatttgct
    10561 gccgggcggg atttcgctcc cccggctgtg actgcaacac ccgctgagaa ttattgttta
    10621 tctggttttc taaatgctgc tgttgcaact gagtttgcgt tttcagttgc tgattcagca
    10681 tccctttttg ctggatttgc tgagtctgca tctgggtttg catccgctgc tggctgggta
    10741 tctgataccc cggctggtta gggttgttca gagtattaat gggctgtgca aagccgacaa
    10801 acggcaggag tgccgtaaga atcagaagtc gtttcatcgc gtatcctcct ctgaagatat
    10861 cctttaagtt tactcgcttc ccgacaaaac gatgattaat tcagagttat ataccaggct
    10921 tagctggggt tgccccttaa tctctggaga ataacgatga taaaaccgac gtttttacgc
    10981 cgggtggcca ttgctgctct gctctcagga agttgtttta gcgccgccgc cgcgcctcct
    11041 gcgccgcccg tctcgtatgg tgtggaggaa gatgtcttcc acccggtacg cgcgaaacag
    11101 ggaatggtag cgtctgtgga cgccactgcc actcaggtgg gggtggatat tctcaaggag
    11161 ggcgggaatg ccgttgatgc cgccgtggcg gtgggctacg cgctggcggt aacgcatccg
    11221 caggcaggga atctgggcgg tggtggtttt atgttaatcc gctcgaaaaa tggcaatacc
    11281 acggctatca atttccgcga aatggcaccc gccaaagcga cccgcgatat gttcctcgat
    11341 gatcagggca acccggacag caaaaaatca ctcacttcgc atctggcttc cggcacaccg
    11401 ggtacggtag caggtttctc gctggcgctg gataaatacg gcaccatgcc gctgaacaaa
    11461 gtcgtgcagc ccgcgtttaa actggcacgc gatggtttta tcgttaacga cgcgctggct
    11521 gacgatctca aaacctacgg tagcgaagtg ttgccgaatc acgaaaacag taaagctatc
    11581 ttctggaaag agggcgagcc gctgaaaaag ggcgacacgc tggtgcaggc gaacctggca
    11641 aagagcctgg agatgattgc tgaaaacggc ccggacgaat tctataaagg cacgattgcg
    11701 gaacagatcg cccaggagat gcagaaaaac ggtggcttga tcactaaaga agatttagcg
    11761 gcctataaag cggtcgaacg cactccgata agcggcgatt atcgcgggta tcaggtttac
    11821 tccatgccac cgccatcctc cggcgggatc catatcgtac aaatcctcaa tattctggaa
    11881 aacttcgata tgaagaaata cggctttggc agcgccgatg cgatgcaaat catggcagag
    11941 gctgagaaat atgcctacgc cgaccgctcg gaatatcttg gcgacccgga ttttgtcaaa
    12001 gtcccgtggc aggcgctgac caataaagcc tatgccaaat ctattgccga gcaaatcgat
    12061 atcaataaag cgaaaccgtc cagcgagatt cgccctggca agcttgcgcc ttatgagagt
    12121 aatcaaacta cccattactc agtggtggat aaagatggta acgcggtggc ggtgacctat
    12181 acgctgaaca ccaccttcgg tacgggtatt gtcgcgggcg agagcggtat tctgcttaat
    12241 aaccagatgg atgatttctc cgccaaaccg ggcgtaccga acgtttacgg gctggtgggc
    12301 ggtgatgcca acgcggtcgg gccgaataaa cgcccgctgt cgtcgatgtc accaaccatt
    12361 gtggtgaaag acggtaaaac ctggctggtt accggtagcc ctggcgggag tcggattatt
    12421 accaccgtgc tgcaaatggt ggtgaatagc atcgattatg gcatgaatgt cgctgaagcg
    12481 accaatgcac cgcgcttcca ccatcaatgg ttgccggacg agctgcgtgt cgaaaaaggg
    12541 tttagcccgg atacgctcaa gctgctggaa gcaaaaggtc agaaagtggc gctgaaagag
    12601 gcgatgggca gtacacaaag cattatggtt gggccggacg gtgagttgta cggcgcatcc
    12661 gacccgcgct cggtggatga tttaacggcg gggtactaag gttagcggcc ctcttcgtgg
    12721 gaagagggct attttgtcag ggcaagccga aggtagcctt ttttatttcg taatcctgta
    12781 gatattcttc cagcggcttt gccgttccca gagaatgaat ttcaccactg tctttatcaa
    12841 taataaaagg tgcgttacca gctaagcgcg cggcctcatc tccagtttcg agaaattctc
    12901 gtgcttcgaa acagaaatac cagccctggc taaagcgtcc atgtagagta atgacgaccg
    12961 ggagatctgc atcatcaagg taatggttcg ctttcg
//
LOCUS       DAZFWP020000136        12881 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFWP020000136 DAZFWP020000000
VERSION     DAZFWP020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.136"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..896
                     /gene="yhfZ"
                     /locus_tag="ACHK0Z_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             <1..896
                     /gene="yhfZ"
                     /locus_tag="ACHK0Z_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGU4503755.1"
                     /translation="FIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQA
                     ALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASG
                     LKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALELGPH
                     TYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESLQRIV
                     KGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLLRAVV
                     DKHALLAHQQRVVSGEQEPSY"
     gene            913..1275
                     /gene="yhfY"
                     /locus_tag="ACHK0Z_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             913..1275
                     /gene="yhfY"
                     /locus_tag="ACHK0Z_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4503756.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            1359..2522
                     /gene="yhfX"
                     /locus_tag="ACHK0Z_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             1359..2522
                     /gene="yhfX"
                     /locus_tag="ACHK0Z_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGU4503757.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIVIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            2522..3748
                     /gene="yhfW"
                     /locus_tag="ACHK0Z_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             2522..3748
                     /gene="yhfW"
                     /locus_tag="ACHK0Z_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4503758.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            3745..4623
                     /gene="php"
                     /locus_tag="ACHK0Z_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             3745..4623
                     /gene="php"
                     /locus_tag="ACHK0Z_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGU4503759.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            4634..4987
                     /gene="yhfU"
                     /locus_tag="ACHK0Z_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             4634..4987
                     /gene="yhfU"
                     /locus_tag="ACHK0Z_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGU4503760.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            4999..6303
                     /gene="yhfT"
                     /locus_tag="ACHK0Z_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             4999..6303
                     /gene="yhfT"
                     /locus_tag="ACHK0Z_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGU4503761.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVKQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            6315..7400
                     /gene="yhfS"
                     /locus_tag="ACHK0Z_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             6315..7400
                     /gene="yhfS"
                     /locus_tag="ACHK0Z_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4503762.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANISTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(7552..8283)
                     /gene="frlR"
                     /locus_tag="ACHK0Z_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /gene_synonym="yhfR"
     CDS             complement(7552..8283)
                     /gene="frlR"
                     /locus_tag="ACHK0Z_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU4503763.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(8383..9168)
                     /gene="frlD"
                     /locus_tag="ACHK0Z_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             complement(8383..9168)
                     /gene="frlD"
                     /locus_tag="ACHK0Z_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGU4503764.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGPI
                     TWVGDDDYGTKLKQDLARMDVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(9165..9995)
                     /gene="frlC"
                     /locus_tag="ACHK0Z_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             complement(9165..9995)
                     /gene="frlC"
                     /locus_tag="ACHK0Z_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU4503765.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(10045..11067)
                     /gene="frlB"
                     /locus_tag="ACHK0Z_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             complement(10045..11067)
                     /gene="frlB"
                     /locus_tag="ACHK0Z_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU4503766.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQHGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(11088..12425)
                     /gene="frlA"
                     /locus_tag="ACHK0Z_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             complement(11088..12425)
                     /gene="frlA"
                     /locus_tag="ACHK0Z_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU4503767.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGAQGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(12720..>12881)
                     /gene="yhfL"
                     /locus_tag="ACHK0Z_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             complement(12720..>12881)
                     /gene="yhfL"
                     /locus_tag="ACHK0Z_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU4503768.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
BASE COUNT         2963 a         3228 c         3652 g         3038 t
ORIGIN      
        1 cgtttatcaa aaaagaaggc gtcgttatca cgacgctggc ccgttatttg ttgggtgaaa
       61 aatgcggtaa taggttgaaa accatagatg agctggcaac tgaatgccgt tcatccgttg
      121 gcctgacgca ggccgcgttg aaaacgctgg aatcaagcgg agcgatacgg attgaacgcc
      181 gtgggcgcaa tggcagttat ctggtcgaga tggataacaa agcattgctg agtcatgtgg
      241 atatcaacaa cgtggtatgt gcgatgcctt tgccctatac ccgcttgtac gaaggtttag
      301 cgagcggatt gaaagcccag tttgatggca ttccttttta ctatgcgcat atgcgcggcg
      361 cggatattcg cgtggagtgt ttacttaacg gcgtgtatga catggcggtg gtttcccgac
      421 tggcggcgga aagttatctc acgcaaaaag ggttttgcct cgcgctggag ttggggccgc
      481 acacctacgt tggcgagcac cagttgattt gccgtaaagg cgagtccgca aacgtgaagc
      541 gcgtggggct ggataaccgt tcggcggatc agaaaatcat gaccgatgtc ttttttggcg
      601 atagtgatgt ggaacgtgtc gatctctctt atcacgagag tttacaacgc attgttaaag
      661 gcgatgtcga tgcggttatc tggaacgtgg tggcggaaaa cgaactgacc atgctgggat
      721 tagaggcgac gccgctcaca gacgatccgc gatttttaca ggccaccgaa gccgtggtcc
      781 tgacgcgagc cgatgattac ccaatgcaac aactgctgcg tgccgttgta gataaacacg
      841 ccctgctcgc ccatcaacaa cgcgtggtga gtggggaaca ggaaccgagt tattaaacga
      901 aaggcatctg atatggaaac cagactcaac ctgctttgcg aggcaggcgt tattgataag
      961 gacatctgca aaggcatgat gcaggtcgtc aacgtactgg aaacagagtg ccatctgcca
     1021 gtgcgcagtg agcaaggaac gatggcgatt acacatatgg cgagtgcact gatgcgcagt
     1081 cgccgtggtg aagaaataga gccgctggat aacgaattgc tggcagagct ggcgcaatcc
     1141 agccactggc aagctgttgt gcaattgcat caggtattgt tgaaggaatt cgcactggaa
     1201 gttaacccgt gtgaagaagg ctatttgctg gccaaccttt atggattatg gatggctgct
     1261 aacgaagagg tttgaagctc ccgacaaatc cacgtcgcac aacctaaata ttcaaaaaaa
     1321 tgaatattta attcaaggaa cagaaacgag gcaacaagat gtttgtagaa gcattgaaac
     1381 gccagaaccc ggcgctgatt tccgccgcac taagtttgtg gcagcagggc aaaatcgccc
     1441 cggacagctg ggtgatcgac gtggatcaga tactggaaaa cggtaagcgg ctgattgaga
     1501 cggcgcggct ttacggcatt gaactgtatc tgatgaccaa acaattcggt cgtaacccat
     1561 ggctggcgga aaaactgctg gcgttgggct acagcggcat tgtggcggtg gattacaaag
     1621 aggcgcgagt catgcgccgc gctggtttgc cggtggcgca tcaggggcat ctggtacaaa
     1681 tcccttgtca tcaggttgct gacgccgttg aacagggcac cgacgtcatc accgtgttta
     1741 ctctcgacaa agcgcgggaa gtttctgcgg cggcggtgaa ggccgggcga atccagtctg
     1801 tgctgctcaa agtttatagc gacgatgatt ttctttatcc ggggcaggag agcggttttg
     1861 tacagcattc gctgcacgag gtggttgctg aaattaagaa actgccgggg ctgcatttag
     1921 ccggacttac ccatttccct tgcctgcttt gggatgaggc tgccgggaaa gttttgccga
     1981 caccgaatct tcacacgctg gtacaagcac gggatcaact ggcgaaatct ggtattgtga
     2041 ttgagcaact gaacgcgcct tcagcgacca gctgcacttc gctgccatta ctggcgcaat
     2101 acggtgtgac tcatgccgaa cccggtcatg cgctgacggg cactattccg gcaaaccagc
     2161 agggcgatca gtctgaacgt atcgcgatgc tctggttgag tgaaatctcc catcatttcc
     2221 gtggcgacag ctactgctac ggcggcggtt actaccgtcg tggtcatgcg caacatgcgt
     2281 tggtgtttac gccagaaaat caaaagatta ctgaaaccaa tctcaaaact gtggatgaca
     2341 gcagtatcga ctacaccctg tcgctggcag gcgagtttcc ggtaagcagt gcagtggtgc
     2401 tctgtttccg cacgcagatt tttgtcaccc gtagcgatgt ggtgctggtg tccggtattc
     2461 accgtggcga accgaaaatc gttggtcgtt atgacagtct tggaaactct ctgggggcgt
     2521 aatggcgcga tttgtggtgt tagtgattga tagctttggc gtaggggcaa tgaaagatgt
     2581 cacgctggtg cgtccgcaag atgcgggagc gaatacctgt ggtcacatcc tgagccagtt
     2641 gccgcatttg cagctaccaa cgctggagaa gctggggcta atcaacgcat tgggttatgc
     2701 gccaggcgat atgcagccgt cagattccgc aacctggggc gtggcagagc tgcaacatga
     2761 aggtggcgat acctttatgg ggcatcagga aattttaggc acgcgcccgt taccgccgct
     2821 gcggatgcct tttcgcgatg tgattgaccg tgttgagcag gcattagttt ccgctggctg
     2881 gcaggtggag cgccgtggcg atgatctgca atttctgtgg gtcaatcagg cggttgcgat
     2941 tggcgataat ctcgaggcgg atttaggcca agtctataac attaccgcca atctctctgt
     3001 gatctctttt gacgacgcaa tcaaaattgg tcgtatcgtg cgtgagcagg tacaggtcgg
     3061 tcgggtcatt acatttggcg gcttgttaac cgacagtcag cgtattctcg atgccgcaga
     3121 aagcaaagaa gggcgcttta ttggcatcaa tgcgccgcgt tctggcgctt atgacaacgg
     3181 tttccaggtc gtgcatatgg gctacggtgt tgatgaaaaa gtgcaggtgc cgcaaaaact
     3241 gtatgaggcg ggcgtgccaa ccgtgctggt aggtaaagtg gcagatatcg tcagcaatcc
     3301 ttatggcgtg agctggcaaa acctggtgga tagccagcgg attatggata tcacccttga
     3361 cgaatttaac acgcatccga cggcgtttat ttgcaccaat atccaggaaa ccgacctcgc
     3421 tggtcatgca gaagacgtcg cacgttatgc cgaacgtttg caggtcgttg accgtaacct
     3481 tgcccggctt gttgaggcga tgcagccaga tgattgcctg gtcgtgatgg cggatcacgg
     3541 taacgatccg accattggtc acagccacca tacccgcgaa gtggtgccag tgctggttta
     3601 tcagcaaggg atgatagcta cgcagctcgg tgtgcgcacc acgctttcag atgtgggggc
     3661 taccgtgtgt gaatttttcc gtgcgccacc gccacaaaat ggtcgctctt ttctttcctc
     3721 cctccggttt gcaggagaca ccctatgagt tttgatccga cgggttacac cctggcccat
     3781 gagcatctgc atattgatct ctccggcttt aaaaacaacg tggactgccg ccttgatcag
     3841 tatgcgttca tttgccagga gatgaacgac ctgatgaccc ggggcgtgcg taatgtgatt
     3901 gagatgacca accgttacat ggggcgcaat gcgcaattta tgctcgatgt aatgcgcgag
     3961 acggggatca acgtggtggc ctgcaccggt tattaccagg acgctttttt cccggagcat
     4021 gtggcgaccc gcagcgtgca ggaactggcg caggagatgg tcgatgaaat tgaacagggt
     4081 atcgatggca ccgacctgaa agccgggatt atcgcggaga tcggctctag cgaagggaag
     4141 attacgccgc tggaagagaa ggtgtttatt gccgctgcgc tggcgcataa ccagaccgga
     4201 cgcccgatct ccacgcatac ttcgttcagc accatggggc tggaacaact ggctctgttg
     4261 caagcccacg gagttgatct ttcgcgcgtc accgttggtc actgcgatct gaaagacaac
     4321 ctcgacaaca ttttgaagat gatcgatctc ggcgcgtacg tgcagttcga caccattggt
     4381 aagaacagtt actacccgga cgaaaagcgc attgcgatgc tccacgcgct acgtgaccgt
     4441 gggttgctga accgcgtcat gctgtcgatg gatattacgc gccgctccca tttaaaagcc
     4501 aatggtggtt atggctatga ctatttattg accaccttta ttccgcaatt gcgccagtca
     4561 ggattcagtc aggccgatgt ggatgtgatg ttacgtgaaa ctccctctca atttttccaa
     4621 taaggacaga ctcatgaaaa agattggcgt tgcaggctta cagcgtgagc agattaaaaa
     4681 aaccattgaa gcgacggctc ctggcagttt tgaagttttc attcacaacg acatggaagc
     4741 ggcaatgaaa gtgaaatccg ggcaactgga ttactacatc ggtgcgtgta ataccggtgc
     4801 gggcgcggca ttgtcgattg ccatcgcggt gattggctat aacaaaagtt gcaccattgc
     4861 caaaccaggc attaaagcga aagacgagca tatcgccaaa atgatcgctg aaggaaaagt
     4921 ggcgtttggc ctttccgttg agcacgtcga acacgcgatt ccgatgctga ttaaccatct
     4981 gaaataaaag gcacgactat ggatctgtat attcagatta tcgtggtggc gtgcctgacg
     5041 ggtatgacat cgcttctggc gcatcgctcg gcggctgttt ttcatgacgg catccgcccg
     5101 atcctgccgc aactgattga aggctatatg aaccgtcgcg aggcggggag tatcgctttt
     5161 ggtctgagca ttggttttgt ggcctcggtg gggatctctt ttaccctgaa aaccggactg
     5221 ctcaacgcat ggttactctt tcttcctacc gatatcctcg gcgtactggc gataaacagc
     5281 ctgatggcgt ttggtcttgg cgctatctgg ggcgtattga tccttacttg cctgttgcca
     5341 gtaaagcagc tgctgaccgc gctgccggtg gatgtattag gtagcctcgg ggaattaagc
     5401 tcgccggtgg tttctgcttt tgcactgttc ccgctggtgg cgattttcta ccagtttggc
     5461 tggaagcaaa gtctggtcgc cgccgtggtt gttctgatga cccgtgtggt agtcgtgcgc
     5521 tatttcccac atcttaaccc tgaatccatc gaaatcttta ttggcatggt gatgctgctg
     5581 gggatcgcga taactcacga cctgcgtcat cgtgatgaaa atgatattga tgccagcggg
     5641 ctttcggtgt ttgaagaacg cacgtcgcgg attatcaaaa acttacccta tatcgccatc
     5701 gtgggagcat tgattgccgc cgttgccagc atgaagattt tcgccggcag tgaagtgtcg
     5761 atcttcactc tggagaaagc gtactccgca ggcgtaacgc cggaacaatc gcaaacgctg
     5821 atcaaccagg cggcactggc agaatttatg cgcgggctgg gttttgtacc gttgattgcc
     5881 accaccgcct tagcaacggg tgtgtatgca gttgcgggct ttacctttgt ttatgcggtg
     5941 ggctatctct cgccgaatcc gatggttgca gcggtattag gcgcagtggt tatttcggcg
     6001 gaagtcttgc tgcttcgttc gatcggcaaa tggctgggac gctacccgtc ggtgcgtaat
     6061 gcgtcggata acatccgtaa cgccatgaat atgctgatgg aagtggcgct gctggtcggt
     6121 tcgattttcg cagcaattaa gatggcgggt tataccggat tctctatcgc ggttgccatt
     6181 tacttcctca acgaatccct gggccgtccg gtacagaaaa tggcggcacc ggtcgtggca
     6241 gtaatgatca ccggtattct gctgaatgtt ctttactggc ttggcctgtt cgttccggct
     6301 taaggaaggc acctatgaag acgtttcctc tgcaaagcct gacgattatt gaggcgcagc
     6361 aaaagcagtt tgcgctggtg gatagcattt gtcgccattt ccccggcagc gagtttctta
     6421 ctggcggtga tttaggctta acgccaggac tgaatcaacc gcgcgttacc cagcgtgtgg
     6481 aacaggtgct ggctgatgca tttcacgcac aggctgcggc gctggtgcag ggcgcgggga
     6541 ctggcgcgat tcgcgccggg ttggcggctt tgctcaaacc ggggcagcgt cttctggtgc
     6601 atgacgcgcc tgtttacccg acgacacggg ttattattga gcagatgggg ctgacgctta
     6661 ttactgttga tttcaatgac ctgtcggcac tgaagcaggt cgtcgacgag caacaaccgg
     6721 atgcggcgct ggtgcagcat acgcgccagc agccgcagga cagctacgtg ctggcagatg
     6781 tgctggcaac gttgcgcgcg gcaggtgttc cagcgttaac cgatgacaac tatgcggtga
     6841 tgaaggtggc gcgaatcggc tgtgaatgcg gcgcgaatat ctcgacattt tcctgcttca
     6901 agctatttgg gccagagggt gttggtgcag tggtcggcga tgctgatgtt atcaaccgta
     6961 ttcgcgccac gctttactcc ggcggtagcc agatccaggg cgcacaggcg ctggaagtct
     7021 tgcgtggtct ggtgtttgcg ccagtgatgc acgcggtgca ggcaggggta tctgaacggt
     7081 tgctggcttt gcttaacggt ggtgcggtgc cggaagtgaa aagcgcggtg attgctaatg
     7141 cgcagtcgaa ggtgttgatt gtcgagtttc atcagccgat tgccgccaga gtgctggaag
     7201 aggcgcaaaa gcgcggtgcc ttgccttacc cggtgggtgc agagtcgaaa tatgaaatcc
     7261 cgccgctctt ttatcgcctt tccggaacgt ttcgccaggc gaatccacaa tcagaacatt
     7321 gtgcgattcg cattaacccg aatcgcagcg gtgaagagac ggtgctgcgg attttgcgtg
     7381 agagtattgc cagtatttaa tgctggatag caccgtcaag ctaaattccg tactgaacgg
     7441 tcccctcgcc cctttgggga gagggttagg gtgagaggaa cgtacgggat gtaaaattca
     7501 acgcgccagc atcgccggat gcgatgctgg cgcatcttat ccggcctacg gttaatgtct
     7561 tttattatca atagttaagg tgatgcgatt ggcgcggcag aagagttcgg agacgtgcac
     7621 cggtttgtca ttctgatcaa aggcgatttt gctgatgcga aacaacggtt cgcccagttc
     7681 gcatttcagc cattttgcct gcgggcgggt ggcggcaaag atatcgatgg tctttttgtc
     7741 gctgaccact cgcgtgtcaa aacgctcctg aaataactga taggtggagc ttccttcgac
     7801 gtaaatctca tcaaagtcag gataacgcga cagcgggatc catgaactat caataaacag
     7861 cggctcttta tcgagataca tcacccggca gagatggaac acttcgctgt ttccggggat
     7921 gttcagcttt tcgcaaaacg gcgcggcgct gacccgttcc tgttcgatca ctttctcttt
     7981 cgtcgccttg ccttgtgaga cgccaaaatc ggtaaaacca ctgacagtaa gcagggcgtt
     8041 ttcaactttc tggctttgta caaaggtgcc ttttccctgc cagcggatca gtacgccgtc
     8101 tgccactaag tcgctgatgg ctttgcgaat ggtaatgcgg ctgacgttat attgtgtaca
     8161 aagctcgttt tcggtaggga tctgttgccc ggcctggtaa accccctgcg cgatatcatc
     8221 cagcagtcgc tggcggacgg tagcgtagag gagttgatga gagtagcggt ccgtagctga
     8281 cataatcgac cctgaaataa agaatgaatt actcggatta tagggtcgtt ttccggcaag
     8341 taacagcacg ttggcgtgaa gatgctcacg ccaacgttat acctaccagg caccgtggta
     8401 ctgaatggtt ttcgccgcgc acgccgttcc ctgcgccatc gcctgcggta atgtcatccc
     8461 cgcagaccag ccgcaaagga atccggcaat gaacgaatct ccggcaccca tggtgtcgat
     8521 aaccgtcacc ggttcaggag cctgacgcca gaactgcgcg ccatcccagg caatgctgcc
     8581 gttttcaccc agcgtgacaa tcactgttcc tgcgccacgg gcaacaatcg ctttcatctt
     8641 cagacgcagc gcttcgtctt cttgcggtgc ggaggcaaag gcaaaatcga gatgcggcac
     8701 cagtgtctgc cagagcgggc tgtcccactt gtcggagaag tcgaaagcgg taagtttgcc
     8761 cgcagcgtgc agctgtggga atgcgtcttc cgcatgtccc cagattgccg cgtgcacaat
     8821 gtcatactgc gccagccagg cgtaatcctc ttcactcagg gcaaagtcgg ccatcacgcc
     8881 ttcggtgtag tcgccaaaaa cgcgatcatt gtcgtgcagt tccacctgag tttgtgcggt
     8941 aacgccgtgt ttcgtatgga catggctgat atcgacgtcc atgcgggcga gatcctgctt
     9001 cagctttgtg ccgtagtcat cgtcacccac ccaggtaatg ggtcccggct gtatgccgta
     9061 gcgagtgcag tacaccgcca cattgaccgc attaccgcca gaaaacgctt tattcagttg
     9121 cgggtagata tcgacgcagt tatcgccgat tgtcgccagg gttttcatct ctcatcctcc
     9181 ggcagcagcg cgcgaaagcg ttccagcgcc tggcgggcat agagtctggg ctcgttcata
     9241 tacatcgtca ccagttccac cgtgcagtaa ccgtcatagc cccggtcgat aatatcgcgc
     9301 atcagctccc gcagcggcat tttgccttcg ccgggaatgt aatgtgtgtc actggccccg
     9361 tcgctgtcga caatatgcag atgacgcaat ttatcaccca gtttgtcgaa ataactcatc
     9421 accggttccg cctggacata cggcgcgcaa atgtcgacca tgctgaacag gcgcggcgaa
     9481 ggcaccagcg ccagcgcatg aagcacatca ttagcattac acacgacgtt cgattcatac
     9541 ggcgttaacg gttcgaggat cagatccatg ccgatgtttt ccgcgtactc acacagttcg
     9601 cttaggttct ctgccagccg tccccagata acattaggtg gcgtgagata gcccgcgtgg
     9661 gccgcggaaa tcagcgtata acccgcgttc atctcttttg ccatatccat cgccagcttg
     9721 atcatgtcga ggctttcgcg acgcatatgt tcatcgccca gcatcatgtt atacggatag
     9781 ccgttggttt ctggcgtata gccgataatc ggcatctgat acgtctgcgc cagcgccttg
     9841 atttgtttga tgccgcccgc ttttaagtcc ggcgcgaacg cgtgcgggcg accgccccaa
     9901 atttcgatgc cgtcgtaacc cagctcgctt gcatcacgaa atgcgtgttc aataggcagc
     9961 cgctggtggc cgcaggtaaa catacctgtt ttcatcgggt aactccttgc gtaaattcgg
    10021 cccgttgccg ggccgggaga gggattaata ttccaccaga ccaccgtaat agcggcgttc
    10081 atccgggttg tgatctttgt aaatagacag gtagtagcag agccactcca ttggcacgaa
    10141 catcaggaac ggtgccagcc acgggtgcag cccttgcgaa atttcggcgt aatcgatgac
    10201 gatcacgttg tcagtacgct gtttaacaaa gttaatggcg cgttcggtgg tgtggcgact
    10261 ttcatcattg ccgagcagga acaggaacgg aacgcccggt tcgacaatct ccagcgggcc
    10321 atggcggaac tctccgctct caatcacgca gccgtgcgtc caggtaaatt ccatcagcgt
    10381 tacaatgcct tctttgtaac ccagcggacg cagcggaccc gcagcaacgg tataaatcat
    10441 cggccactgg ctggccagtt caccatgctg gcggcctttt tcttcccagg tgcgtaccag
    10501 atgaccgagc gcattcggca actgcttgag atcgtttttg atcttgccga tttccgcgtt
    10561 cggcgcgagg cgggtgatca tctccagcac cacgctgtag cagagcagca ggtgaatttc
    10621 ccagatacag tcggcctgat aatcaatgct aaattccgcc gccgaggtaa tcgggctgtc
    10681 cgcgcgtttg gtgaacgccg cagtgagtgc gccgcaggcc cggcccagct ccagcgcttt
    10741 gattacctct tccgttttac cgtagtcaga aacgccaatc accgcgcaac gatcgtcgag
    10801 gcgatacggg gtgttatcgc agaactccca gccggaaatg gcgtagacct gaagatcgga
    10861 aaagcgatcc gccagatgtt tcgccgtttg cgccgcgttg agtggcgatc cgcaggcaac
    10921 gaaataaata cgatcaatgt cgcgcttcac catctcttcc acgatggtgt gcaccagcgg
    10981 aacgtcatgg ctgagaactt tttccacttc ctgaaccata ttttcggtga ccagaaagtc
    11041 cacggtgctt ttatcaatat ccaacatttt attttctcca gataacatta cgacaaagcg
    11101 ttgagctggc ggctacgctt cgcccagaag gcgtaagcag gcagaccagt agcaataacg
    11161 atgacggcgc agataaggcc gggaatcggt gcccagacaa aggttgaggc gaccagaatg
    11221 aggcttgacg caatggcgag ggtggtcatc agcccgaaag ccggagtacg ccacagcggt
    11281 ttgtaatcgt cgcgtttacg acaccagatg atggagccga aggtgagggt atttttgaaa
    11341 cacatcacca gggtgaaata acccagcagg ctggtgagat cggaaacgaa gatgaagaag
    11401 atcccctgcg ccccttgcag gatgatggag acatccggcg tgttgtattt cggatgcaca
    11461 tggccgaagc atttaaagaa caggttgtct ttcgccatcg cgtattccag ccgcggctgg
    11521 tacatcacgc agctggaaag cgaaccaaga atgacgatca tcgccgtgat ggcaacaaag
    11581 atcccagcgg tgctgccgag tgcggggatc caggtcaggg cgtcggaaat cggcgtttca
    11641 gaattggcga gtttgtcgaa gggcatcagg ccggaaatca ccagcgccag cagggtgtag
    11701 agcaccagaa ccagcagaca ggaaccaatc agcgctcgtg gcatggtttt tccggggttt
    11761 ttaatttcgc cggtcatata acagatagag gccatgccgg tatacgacca actggtggca
    11821 gagatccccg ccagcagcgc cataaagctg cccgttgcgc caatcgcagt ggtggtaggg
    11881 gcggcaaaat tctccgcttt aaaccagaag atcccaaggc caatgacgat agtgaacggg
    11941 ataattttgg cgatggtaat tagcgtctga aacgctgcgc cgccttcaac ggagcgcagg
    12001 tgtagcaaca taaaggcgat aattaatccg gcggcgataa atttaccgag caacggatcg
    12061 ataggcgtta aaaagccaag attgctgaca atcgccagcg ccataatcga caatgacggc
    12121 gcatcgttgg cccagaagct ggcccagccg gagaggaaag ccagcggtcg gcttccggca
    12181 tttttcagat aaacataatc tgcgccattt tccggataag cggtggatag ttccgcatag
    12241 acgcacattt gcgggatcac aattaacccg ccaatgacaa acgcgagcac cgtaagccac
    12301 ggcgtgcccg ctgcttttgc cacttcaccc acagatacaa aaataccgga gccgacggtt
    12361 gtcccgacgg cgattgcaag aacggcccaa aatccgagct tgcgttggag ttcctggctt
    12421 cccatagcga tacctttttc ttgtccaata cgttgtaggg gagagtttgc agtcatttaa
    12481 aaggtaacat gacaatgcat gatgaatata acaacatatg atgttatgta atgtggagga
    12541 agatcacaaa atttcgcaca ggatcgcgct gtggctaatg gatgtagtta tcaaattgaa
    12601 tttaaagtga aaatattttt acgggcgggg gcaagaagga tatagaaaca aatacgccct
    12661 cggaaaatcc agagggcgtc gggcaattaa accggtgtta gccgatttct gtcagagact
    12721 tactgtgcag taggaccgca accgccaatg attttagaaa tagaaattgc cgggtgcagc
    12781 aggtagtcgt aagagcagtt cttatcacgg ttttcaatat gaccagtaca tgcagttaac
    12841 gtagccagta ctgcacctac cagtgcaact ttaataaatt t
//
LOCUS       DAZFWP020000137        12842 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFWP020000137 DAZFWP020000000
VERSION     DAZFWP020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.137"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(40..366)
                     /gene="ypeC"
                     /locus_tag="ACHK0Z_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             complement(40..366)
                     /gene="ypeC"
                     /locus_tag="ACHK0Z_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU4503769.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(481..1737)
                     /locus_tag="ACHK0Z_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             complement(481..1737)
                     /locus_tag="ACHK0Z_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU4503770.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            1941..2906
                     /gene="glk"
                     /locus_tag="ACHK0Z_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             1941..2906
                     /gene="glk"
                     /locus_tag="ACHK0Z_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU4503771.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3125..3451
                     /locus_tag="ACHK0Z_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             3125..3451
                     /locus_tag="ACHK0Z_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4503772.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            3473..4720
                     /locus_tag="ACHK0Z_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             3473..4720
                     /locus_tag="ACHK0Z_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4503773.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            4735..5820
                     /gene="ypdF"
                     /locus_tag="ACHK0Z_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             4735..5820
                     /gene="ypdF"
                     /locus_tag="ACHK0Z_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4503774.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            5820..6857
                     /gene="ypdE"
                     /locus_tag="ACHK0Z_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             5820..6857
                     /gene="ypdE"
                     /locus_tag="ACHK0Z_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4503775.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            6882..9377
                     /gene="ptsP"
                     /locus_tag="ACHK0Z_RS19135"
                     /old_locus_tag="pgaptmp_003827"
     CDS             6882..9377
                     /gene="ptsP"
                     /locus_tag="ACHK0Z_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4503776.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDRDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9380..10237)
                     /locus_tag="ACHK0Z_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             complement(9380..10237)
                     /locus_tag="ACHK0Z_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4503777.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10250..10984)
                     /gene="ypdB"
                     /locus_tag="ACHK0Z_RS19145"
                     /old_locus_tag="pgaptmp_003829"
     CDS             complement(10250..10984)
                     /gene="ypdB"
                     /locus_tag="ACHK0Z_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU4503778.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(10999..12696)
                     /gene="ypdA"
                     /locus_tag="ACHK0Z_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             complement(10999..12696)
                     /gene="ypdA"
                     /locus_tag="ACHK0Z_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU4503779.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3035 a         3301 c         3489 g         3017 t
ORIGIN      
        1 agcgtcgcat caggcattgt gttccattgg cgaatgtgtt taatggcggt gtccatgtcc
       61 gcggcctttt ccgcgatgat cgtcacgatc gcgccagcct tcacgatagc cacgctcata
      121 cgctttacgc ttatcccagc cacgatgata gccattatca tgacgccacc agcggttttt
      181 acgccactca taattgcgat gccagtagtc acggtcgcgc cagtgaccac cgtcccagta
      241 attaccgtaa tgatcgcgat cgccaatttg taatttgatt gatggcagta gggtgatttc
      301 acctgcgttt gctgcaagcg gggtaaatgc catcagggcg gccgccagaa acagtgacct
      361 gaacattgtt attctccttc acgatcgaag ccgtcagcgg cctgttaacg caatattacg
      421 gggaggtaaa gccccgtatc atcgccataa ctcttaaatc ataaaggaga gcattttttg
      481 ctaaacgtta tcgtggggtg gctgttgctt cggacgattg accatcatca tcggcttacc
      541 tgctaataac agccatgccg ggagcatgac gatacagagc agcggtagca atgtggtatt
      601 gggtacaacg accgccgcca taaaaagact taaccagcca tcgcgtgtta ccaccagcac
      661 gatgccgaga atagcgcaag aaacggttat tgccgctggt acggcgggta cgtgctcatg
      721 cagcatcaac cctaatgcca cgccgacaaa cactgccggg aagattcgcc caccgcgaaa
      781 gccactggcg gcagcaacga ccagggcggc aagtttaatt accgccagca aaaagtaatc
      841 gctggtgctg aaagcctgat ttgccaccat ctgctgcatc tcatccagcc ctttaaacag
      901 cgaaactggt ccaccaataa cccccagaat accgagaata aatccgccaa tacccagcac
      961 gagcaccgga tttttcattt gattcatcat cgcgtgcaac cgtggtaagc accatacggc
     1021 gaccattcct gctgcgatgg caatcgccgc gacaattgca ccgctgagaa tatcggtcat
     1081 ttccatctgt ccgtaatgcg caatgggcag tgaaaaatgg ggatggaaaa ataatccggt
     1141 agtaagtgca ccagctgctg ccgccattaa tggcgcaaaa agacgatccc atagcggaac
     1201 ttcactactg ccatttaagg tttgcgaaaa tatcaacgcc gccgcaacag gtgtaccaaa
     1261 cagcgcgccg atggttccgg cagaggctaa aatagtccac tccattcggt tgacgcgcgg
     1321 taacagacga gcgccaatgg cgacggcaag ggcgatattg acggtcatga tcggatgttc
     1381 tggccccagg ctgacgccgc cagcaagacc gagaattaat gctacgataa gtcctggtag
     1441 cgcggaaggt ggaaccggtg cgccgatcag cggttcacag gcggggtctg gtccggcatg
     1501 accctggctg aagcggataa ccaaccccac cgcaatacct gttagcgtta atacaccgat
     1561 gatccagaaa ggtgaatcct gggctatccc cagagttccc ggcagtcgtt gccagagcaa
     1621 attctgtaat accgaggcga ttttcatcac cacaatcaga ataagactgg acgcaatccc
     1681 aattgccacg gcggggagcg ataataacaa catggttctg gctcgcggat ggagcatgtt
     1741 ctattcctta tgcggggtca gatacttagt ttgcccagct tgcaaaaagg catcgctgca
     1801 attggtgctg aaacgataaa gtaattgtgt gacccagatc gatatttaca gggagcctgc
     1861 ctttccggcg ttgttgttat gcccccaggt atttacagtg tgagaaagaa ttattttgac
     1921 tttagcggag cagttgaaga atgacaaagt atgcattagt cggtgatgtg ggcggcacca
     1981 acgcacgtct tgctctgtgt gatattgcca gtggtgaaat ctcgcaggct aagacctatt
     2041 cagggcttga ttaccccagc ctcgaagcgg tcattcgcgt ttatcttgaa gaacataagg
     2101 tcgaggtgaa agacggctgt attgccatcg cttgcccgat taccggtgac tgggtggcaa
     2161 tgaccaacca tacctgggcg ttctcaattg ccgaaatgaa aaagaatctc ggttttagcc
     2221 atctggaaat tattaacgat tttaccgctg tatcgatggc gatcccgatg ctgaaaaaag
     2281 agcatctgat tcagtttggt ggcgcagaac cggtagaagg taaacctatt gcggtttacg
     2341 gtgccggaac agggcttggg gttgcgcatc tggtccatgt cgataagcgt tgggtaagct
     2401 tgccgggcga aggcggtcac gttgattttg cgccgaatag tgaagaagag gccattatcc
     2461 tcgaaatatt gcgtgcggaa attggtcatg tttcggcgga gcgcgtgctt tctggccctg
     2521 ggctggtgaa tttgtatcgc gcaattgtga aagctgacaa ccgcctgcca gaaaatctca
     2581 agccaaaaga tattaccgaa cgcgcgctgg ctgacagctg caccgattgc cgccgcgcgt
     2641 tgtcgctgtt ttgcgtcatt atgggccgtt ttggcggcaa tctggcgctc aatctcggga
     2701 catttggcgg cgtgtttatt gcgggcggta tcgtgccgcg cttccttgag ttcttcaaag
     2761 cctctggttt ccgtgccgca tttgaagata aagggcgctt taaagaatat gtccatgata
     2821 ttccggtgta tctcatcgtc catgacaatc cgggtcttct cggttccggt gcacatttac
     2881 gccagacctt aggtcacatt ctgtaaatcc ttcctgttat atcgggaggt aactctcccg
     2941 ataatctttt aaatcataca gttaattcaa tttttctttg tgtcccctca caaggccgac
     3001 ctgcgtcaca cttccataca gcgggattaa ttctccagta aatgcattat ttgtctggta
     3061 acggcgattt gttttgcacg ttcataattt cactcgtcaa ctatcactga acgaggaatt
     3121 aacgatgagt aagaaactga ttgccttatg tgcctgcccg atgggcctgg ctcacacttt
     3181 tatggccgct caggcgctgg aagaagcggc ggtagaagcc ggttatgaag tgaaaattga
     3241 aacccagggc gcggacggca tccagaatcg cctgacggcg caggatatcg ccgaagcgac
     3301 catcatcatc cattccgtgg cagttacccc ggaagataac gaacgtttcg aatcacgcga
     3361 cgtttatgaa atcactttgc aggacgcaat taaaaacgct gtaggcatca tcaaagaaat
     3421 cgaagagatg attgcctctg aacaacagta atattcgaca gggataattg ttatggccat
     3481 taaaaaacgc agtgcaaccg ttgtgcctgg cgcatccggt gcggcagcgg cagttaagaa
     3541 tccgcaggcc tctaaaagca gcttctgggg tgaactcccg cagcatgtga tgtcagggat
     3601 ttcacgcatg gtgccgacct taattatggg cggtgtgatc ctcgctttca gccagctgat
     3661 tgcttatagc tggcttaaaa ttcctgctga gatcggcatc atggatgctc ttaatagcgg
     3721 gaaattctcc ggtttcgacc tctccttact gaaatttgcc tggctgtcgc agtccttcgg
     3781 cggcgtgttg tttggctttg ctattccgat gtttgccgct tttgtggcga actctatcgg
     3841 cggcaaactg gcgttcccgg ctggttttat cggcggcttg atgtctaccc agccgacgca
     3901 actgctgaac ttcgatccca gcactatgca atgggcgacc tcttcgccgg tgccgtccac
     3961 cttcattggt gcgctgatta tatctatcgt tgctggttac cttgtgaaat ggatgaacca
     4021 gaaaatccag ttaccagatt tcctgttagc gttcaaaacc acatttttgc taccgattct
     4081 ttccgccatt tttgtcatgc tggcgatgta ctatgtcatc accccatttg gtggctggat
     4141 caacggcggt atccgtaccg tgctgactgc cgcaggtgag aagggcgcgc tgatgtatgc
     4201 aatggggatt gccgcggcga ccgcaatcga ccttggtggc ccaatcaaca aagcggcagg
     4261 tttcgttgcc ttcagcttta ccaccgacca cgtattaccg gttaccgcgc gttctatcgc
     4321 tatcgttatt ccgccgattg gtctggggct ggcgaccatt atcgaccgtc gtttaaccgg
     4381 caaacgcctg ttcaacgctc aactttatcc gcagggtaag accgccatgt tcctggcgtt
     4441 tatggggatc agtgagggtg cgattccgtt tgcgctggaa agccccatca ccgccattcc
     4501 gtcgtatatg gtcggcgcta ttgtcggctc aaccgccgct gtctggctgg gcgcagtgca
     4561 atggttccca gagtccgcta tctgggcatg gccgctggtc actaacctgg gcgtctatat
     4621 ggcggggatc gcgctggggg cagtcataac tgcgctgatg gtggtgttcc tgcgtctgat
     4681 gatgttccgt aaaggcaaat tgttaatcga tagcctgtaa gaaggaaaat ccagatgaca
     4741 ttactcgctt cgctgcgcga ctggcttaag gcgcaacaac tggatgcagt gcttctctcc
     4801 tcacggcaga acaaacagcc gcatctgggg atctccaccg gatcaggcta tgtggttatt
     4861 agccgtgaaa gtgcgcacat tctggtggat tcgcgctatt acgttgaggt ggaagcccgt
     4921 gcgcaaggct accagctgca tttgcttgac gcgacgaaca cgcttaccac tatcgtcaat
     4981 caaatcattg ccgatgagca gttgcaaacg ctcggttttg aaggccagca ggtgagttgg
     5041 gaaaccgcgc accgctggaa gtctgaactc aatgcgaaac tggttagcgc cacgccggat
     5101 gtgctgcggc aaatcaaaac gccagaggag gtggagataa tccgcctcgc ctgtgggatt
     5161 gccgatcgcg gagcggagca tattcgccgc tttattcagg cggggatgag cgagcgcgag
     5221 atagccgctg aactggagtg gtttatgcgc cagcagggcg cagaaaaagc ctcttttgac
     5281 accattgtcg ccagcggctg gcgcggggcg ctgccgcacg gcaaagccag cgacaagatt
     5341 gttgcagcgg gcgagtttgt cactctcgat tttggtgcgc tgtatcaggg ctactgctct
     5401 gatatgacgc gcaccttgct ggtgaatggc gaaggggtga gcgccgaatc tcacccgctg
     5461 tttgacgtct atcagattgt cctgcaggca cagctcgcgg caatctctgc aattcgcccc
     5521 ggcgtgcgct gccagcaggt tgacgatgcc gcgcgccaag tgattaccga ggcaggtttt
     5581 agccactatt tcggtcataa caccggtcat gctatcggca ttgaagttca tgaagatccg
     5641 cgtttttcac cgcgggacac cacgacgcta cagccaggca tgttactgac cgtggagccg
     5701 gggatttatt tgccagggca agggggcgtg cgcatcgaag atgttgtgct ggttaccccg
     5761 caaggcgcag aagtgctcta cgccatgccg aaaacagtgt tgctcacggg agaggcataa
     5821 tggatttatc gctattaaaa gcgttgagcg aggcagatgc gatcgcctcc tcggaacagg
     5881 aagtgcggca gatcctgctg gaagaagcgg atcgcctgca aaaagaagtg cgatttgatg
     5941 gtctgggatc ggtgctgatc cgcctcaatg aatcgacagg tccgaaggtg atgatctgcg
     6001 cccatatgga cgaagtggga tttatggtgc gtagcatctc ccgcgaaggg gcaattgatg
     6061 tgctgccggt tggcaacgtg cgcatggctg cccgccagct gcagccggtg cgcatcacca
     6121 cccgtgaaga gtgcaaaatt ccaggcctgc ttgacggcga ccggcagggg aatgacgtca
     6181 gcgccatgcg cgtggacatt ggtgcgcgct cgtatgacga agtgatgcag gcgggaattc
     6241 gtccaggcga tcgcgtcacc tttgatacaa cttttcaggt tctccctcac cagcgggtga
     6301 tggggaaagc ctttgatgat cgcctcggtt gctatctgct ggtgacgtta ctacgtgagc
     6361 tgcacgatgc cgaactgcct gcggaggtgt ggctggtggc aagttccagc gaagaggtgg
     6421 gattacgcgg tgggcaaacc gctacccgcg cggtgtcgcc ggacgtcgcc attgtgctgg
     6481 ataccgcctg ctgggcgaaa aactttgatt atggcgcggc taaccatcgc cagattggta
     6541 acggcccgat gctggtgtta agcgacaagt cactgattgc gccgccaaaa cttaccgcct
     6601 ggatcgaaac cgtggcggct gaaattggcg taccgttgca ggcagatatg ttcagcaacg
     6661 gcggcacgga cggcggagcg gtgcacttaa ccggtactgg cgtacccaca gtggtaatgg
     6721 ggcctgccac ccgccacgga cattgcgcgg catcgattgc cgattgccgc gacattttgc
     6781 agatggagca acttttatct gcccttattc aacgtcttac ccgtgagacg gttgttcaac
     6841 tgacggattt cagatgatct cctgattaac ccggagcggt tatgttaacg attcaatttc
     6901 tctgtccttt gcctaacggt ctgcatgctc gtccggcgtg ggaacttaaa gaacagtgca
     6961 gccagtggca aagtgaagtc acctttatta accatcgcca gaacgcaaag gccgatgcga
     7021 aaagttcgct ggcgctgatt ggcaccggca ccctatttaa tgacagttgc agcctgaaca
     7081 ttagcggcag cgatgaagag caggcgcggc gcgtgctgga agagtacatc caggtgcgct
     7141 ttatcgacag cgacagcgtc cagcctacgc aggcagaact gacggcgcat ccgctgccgc
     7201 gttcattaag ccgccttaac ccggatttac tgtacggcaa tgtgctggca agcggcgtcg
     7261 gcgtgggtac gctgaccctg ttacaaagcg acagcctcga cagttatcgg gcaatccccg
     7321 ccagtgtgca agattccacc cggctggagc acagcctggc aacgcttgcc gagcaactga
     7381 accagcaatt gcgtgagcgt gacggcgaaa gcaaaactat cctcagcgcc catttgtcgc
     7441 tgattcagga tgatgaattt gcaggcaata tccgtcgcct gatgacagaa cagcatcagg
     7501 gactgggggc ggcgatcatc agcaatatgg agcaggtttg cgccaagctt tctgcttctg
     7561 ccagcgatta tctgcgcgaa cgtgttagcg acattcgcga tattagcgaa cagttgctgc
     7621 atatcacctg gccggaactg aagccgcgca acaatctggt gctggaaaaa ccgaccattc
     7681 tggtggctga agatttaacc ccaagccagt ttttgagcct tgatttgaaa aatcttgcgg
     7741 gcatgattct ggagaaaacc gggcgcacct cgcataccct gattctggcc cgcgcctcgg
     7801 cgatcccggt actgagcggc ctgccgctgg atgcgattgc ccgttatgcc gggcaaccgg
     7861 cggtgcttga cgcccagtgc ggcgtgctgg cgattaaccc gaatgacgcg gtgagcggtt
     7921 attatcaggt tgcgcagacg ctggcggata aacgccaaaa acaacaggcg caggctgccg
     7981 cgcagctggc ctattcccgt gataacaagc gtattgatat cgcggcgaat attggcaccg
     8041 ctctggaagc accaggcgcg tttgccaacg gcgcggaagg cgtcgggctg ttccgtaccg
     8101 aaatgctgta tatggatcgt gacagcgcgc cggacgagca ggagcaattt gaagcctacc
     8161 agcaggtgct actggcagcg ggcgacaagc cgattatctt ccgcacaatg gacatcggag
     8221 gcgataaaag cattccttat ctgaatattc cccaggaaga gaacccgttc ctcggctatc
     8281 gcgcggtacg tatttacccg gaatttgctg gcctgttccg cactcaacta cgggcgattt
     8341 tgcgcgctgc cagttttggc aacgcccagt tgatgatccc gatggttcac agcctcgatc
     8401 agatcttatg ggtgaagggc gagatccaaa aagcgatcgt tgagcttaag cgcgatggcc
     8461 tgcgtcatgc agagacgatt actctgggta tcatggtgga agtgccgtcg gtgtgttaca
     8521 tcatcgacca cttctgcgat gaggtcgatt tcttcagtat cggttctaac gatatgaccc
     8581 agtatctgta tgcggtcgat cgtaataacc cgcgcgtatc gccgctgtat aacccgatta
     8641 cgccatcgtt cctgcgcatg ttgcagcaga ttgttaccac tgcgcatcag cggggcaaat
     8701 gggtaggcat ttgcggtgaa ctgggcggtg aaagccgtta tctgccgcta ctgcttgggc
     8761 tgggcctgga tgagctgagt atgagtagcc cgcgtattcc ggcggtgaaa agccagcttc
     8821 gtcaactgga tagcgaggcg tgtcgggaac tggcgcgtca ggcatgtgaa tgccgcagtg
     8881 cgcaggaaat tgaagcgtta ctcaccgcct ttacgccgga agaagacgtt cgcccactgc
     8941 tggcgctgga gaatatcttt gttgatcggg attttagcaa taaagagcag gcgatccagt
     9001 tcctgtgcgg caacctcggc gttaacgggc gcactgaaca tccgtttgag ctggaagaag
     9061 atgtctggca gcgggaagag attgttacca ccggcgttgg ttttggcgta gcgatcccgc
     9121 acaccaaatc tcagtggatc cgtcattcca gtatcagcat tgcccggctg gcgaaaccga
     9181 ttgactggca gtcagaaatg ggcgaagtcg aactggtgat catgctgacg ctgggtgcta
     9241 acgaagggat gaatcatgtg aaagtcttct cgcagctggc gcgtaaactg gtgaataaaa
     9301 acttccgcca gtcgttgttt gccgcgcaag atgcacaaag tatcctgacg ctgctggaaa
     9361 cagaattaac cttctgacgt tagccctgaa aacgggcgct gtactctccc ggcgtcagac
     9421 caaactgacg ccggaaaacg cgacaaaaat agtcgctatc cggaaaaccg caacgctgtg
     9481 ccacttcatg aattgacaga tgatatttct gcaaaatcat cctcgccttc gccattcgca
     9541 cccaacgcac atactcgata aaacgcatcg ttccatgctg agcaaacagt tttgacagat
     9601 gattgggcgt gatattaaaa aactgcgcca cgctctcgcg ggtgagcggc tgggcataat
     9661 tatcctgaac ccagttacaa atgctgtgat agagaaaatc gccgcgcggc tgcgaattgc
     9721 ccggcggcat attcaccacg ttacggcaaa gatgcagcaa acttaacacc agtggctgaa
     9781 tgatattctg ctccagcggc gaacggctaa gatgggtgag ggcggtaagc atcgcttcac
     9841 cttcaccgcg ttgcaggctg ggaagctgga tctggcgagc aggatgcaac aacgacgtgg
     9901 tgcggctatc gtaaaacgat aacccaagcc aggtcggtgc gaacaccagg cttaacagca
     9961 tcaccggttt gttgttgatc ggtaaattag ccgcgcgtgc cggaataaac agcatttctc
    10021 cttccaccat tcgctgctgc tttgcttcca gcaggttgcc gtattcgccg cgcagcacga
    10081 tatccagacg gggaaaatca atacataaac tgcccaccgg taacgaggca ggctggctgg
    10141 caaaccagac gcgccccagt agttgcgggt taagcaccag gccgctgaac agatcggcga
    10201 aaaattgttg atcggcgggc aagcctggtg ctttcatgaa agttcccgat taaagatgca
    10261 ttaactggcg aaattctttc actttgctgc ggctgaccgg cacttcaaaa tccagatctt
    10321 tcagtcgcag aatgtaggtg ttattaaacc acggttcgat ttcgcgtatt ttgttcagat
    10381 tgacacaaaa tgagcgatgg cagcggaaaa aatgcgacgg cggcagtttg ctgcaaaatt
    10441 cggtaatgtt catcggcatt acgtaggatt cacgccgcgt atagacaaag gtcattttct
    10501 cgtgcgcttc ggcgtaatag atatcgttaa ttggcgtgac gattattcgc tcatctttca
    10561 ccagattaat ggtgtcattc tcacgcgtta ccgtcgcggc aggcgtactg ctggtctgct
    10621 gttgttgcca ggccgcttcc agtttttgca gcatcccggt aatacgtgac tcctgatacg
    10681 gtttgagaat gtagtcaaac gcctccagtt caaacgcttc taccgcatgt tctttccacg
    10741 cggtgatgaa cacaataaac ggtttatggg cgaactggct gatgttttgc gccagcaaca
    10801 cgccatccag cgacggaata ttgatatcca gaaaaatggc gtcgacgcgg ttatgctgca
    10861 aaaacttcaa cacgtccaga ccgtcgtcaa aggtgccgac aatctccatc tggctgtgct
    10921 ctttaattag ccagctcagt tcctgttgtg ccaggaattc gtcttcaaca atgatgactt
    10981 tcacaatatc actccggctc aaagcaataa cgtagcctgt gaggcgactg gggtgcgttg
    11041 gttaggaatg taaaacgcaa tttccgttcc tggttccagg cggcggatat gcagcccctc
    11101 gccatacaat aacttcacgc gatgatgaac attcagcagg ccaattttat tgcccggcat
    11161 ttcattcgct tcgacccgct caatcacctt tggatcgatg ccgtgcccgg tatcccgcac
    11221 cgcaatgcgt acccgattgc cgcactctgc aacgctgatg gtgacaacgc ctttaccttt
    11281 gcaaggctga ataccgtgga caatggcatt ctccaccaac ggctggatca gcaggctggg
    11341 aatgcagcaa ttcacctctt catcaatatc atagataacc gtcagcttgt cgccaaagcg
    11401 ggcctgctca atggcaatat agtctttaat ttgatacagc tcttttttga tatcgatttg
    11461 ctcatcgtct tttaattcaa tgttatagcg cagataacgc gacagattaa agatcagctg
    11521 gcgagcagta tccggattca gacggattga tgatgaaata gcgttcaaag cgttaaacag
    11581 gaaatgggga ttaattttgc tttgcagggc gcgaagctct gccttatttg ccatttcacg
    11641 tagctgctcg gcgcgtgaaa cctccagttg ggtggagatg atttgcgaca gaccgaccgc
    11701 catttcctgt aatgacgagg tgatctgatg cgcgtggcag tagtaaattt tcagcgttcc
    11761 ggtcacaacg cctttctccc acaatgggat caccagcatg gaatgaatct ctggtgtgcg
    11821 gtgggcttca tcattgtttt taatgatgat ttttccgtaa ttcatcgcct gacgggtagt
    11881 cgggctaatg aagtcatcgc cattctgata gttatgttca cccacgccaa cataggccag
    11941 cacatgatcg gtattggtaa tcgccaccgc atcggcgtga atgtcatcgc gaataatttc
    12001 acaaactttg cgtaatgact cgctattgac atggcgaaac agaggtagcg ttttgttggc
    12061 gatatccagc gccagcttgg cctgccgcgc ggcgctggcc tctttttcgc cctcaacgct
    12121 ttgcaccaga agcacaataa agccgataca gacgctaccg aggatcattg gaatgccgat
    12181 tttagagacg atatcgatcc ccaacgcggt agttggtgcc cagacaatta ccagaatcat
    12241 ggtcaacgtc tcacacaaca tgccgccgag aataccgacg cgccagcgct gtgctttggg
    12301 gattttcagg tttatccagc ctgatataca acccgccaga atgctggtga taaagcaggg
    12361 gatcgccgtc acgccgccga tatcaattaa ataccggtga atacccgcaa tcacgccggt
    12421 aatgatgcct acccacgggc caaacagaat cccgccggac atcaccgcga taatacgtac
    12481 gttcaccagc gagccttcta cgggaacgcc ggaccaggtg ctgaacaggg cgaacagcga
    12541 gaaaatggcg gtaacggcga gcaattcttt tggggagtgc gccgacttgt gcaacagttc
    12601 gcgaaacagg cggatacgga tgagaaagaa caggcagata agcattaacg ctgcccgatc
    12661 gaagaccgcc agcagcatgt tgaatatttc gtgcacgggt gactctgaat aaggattaaa
    12721 cctctatgat aggtaacctg aaggctgatg accagcaggc cgtttttgag gaaaaaccat
    12781 aaatttaaaa ggggattgaa agataaatgg tgtattagca atgtatctct tgataatgca
    12841 ct
//
LOCUS       DAZFWP020000138        12811 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFWP020000138 DAZFWP020000000
VERSION     DAZFWP020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.138"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            144..689
                     /locus_tag="ACHK0Z_RS19155"
                     /old_locus_tag="pgaptmp_003831"
     CDS             144..689
                     /locus_tag="ACHK0Z_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4503780.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            664..2589
                     /locus_tag="ACHK0Z_RS19160"
                     /old_locus_tag="pgaptmp_003832"
     CDS             664..2589
                     /locus_tag="ACHK0Z_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4503781.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2586..2792
                     /locus_tag="ACHK0Z_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             2586..2792
                     /locus_tag="ACHK0Z_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4503782.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2789..4390
                     /locus_tag="ACHK0Z_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             2789..4390
                     /locus_tag="ACHK0Z_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4503783.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFVQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4371..5690
                     /locus_tag="ACHK0Z_RS19175"
                     /old_locus_tag="pgaptmp_003835"
     CDS             4371..5690
                     /locus_tag="ACHK0Z_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4503784.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5700..6032
                     /locus_tag="ACHK0Z_RS19180"
                     /old_locus_tag="pgaptmp_003836"
     CDS             5700..6032
                     /locus_tag="ACHK0Z_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4503785.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6088..7113
                     /locus_tag="ACHK0Z_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             6088..7113
                     /locus_tag="ACHK0Z_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4503786.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7155..7553
                     /locus_tag="ACHK0Z_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             7155..7553
                     /locus_tag="ACHK0Z_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4503787.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7565..7918
                     /locus_tag="ACHK0Z_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             7565..7918
                     /locus_tag="ACHK0Z_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4503788.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7930..8508
                     /locus_tag="ACHK0Z_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             7930..8508
                     /locus_tag="ACHK0Z_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4503789.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8505..8900
                     /locus_tag="ACHK0Z_RS19205"
                     /old_locus_tag="pgaptmp_003841"
     CDS             8505..8900
                     /locus_tag="ACHK0Z_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4503790.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8908..9648
                     /locus_tag="ACHK0Z_RS19210"
                     /old_locus_tag="pgaptmp_003842"
     CDS             8908..9648
                     /locus_tag="ACHK0Z_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4503791.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPSSTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            9664..10086
                     /locus_tag="ACHK0Z_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             9664..10086
                     /locus_tag="ACHK0Z_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4503792.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            10068..10502
                     /locus_tag="ACHK0Z_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             10068..10502
                     /locus_tag="ACHK0Z_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4503793.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10495..12811
                     /locus_tag="ACHK0Z_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /pseudo
     CDS             10495..12811
                     /locus_tag="ACHK0Z_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         2946 a         3277 c         4014 g         2574 t
ORIGIN      
        1 ttaaggcgtt tccgttcttc ttcgtcgtaa cttaatgttt ttatttaaaa taccccctga
       61 aaagaaagga aacgacaggt gctgaaaacg aacttttggg cctttgtcgt ttcctttctc
      121 tgtttttggc cgtggaatga acaatggaag tcaacaaaaa gcagctggct gacattttcg
      181 gtgcgagtat ccgtaccatt cagaactggc aggaacaggg aatgcccgtt ctgcgaggcg
      241 gtggcaaggg taatgaggtg ctttatgact ctgccgccgt cataaaatgg tatgccgaaa
      301 gggatgctga aattgagaac gaaaagctgc gccgggaggt tgaagaactg cggcaggcca
      361 gcgaggcaga tctccagcca ggaactattg agtacgaacg ccatcgactt acgcgtgcgc
      421 aggccgacgc acaggaactg aagaatgcca gagactccgc tgaagtggtg gaaaccgcat
      481 tctgtacttt cgtgttgtcg cggatcgcag gtgaaattgc cagtattctc gacgggctcc
      541 ccctgtcggt gcagcggcgt tttccggaac tggaaaaccg acatgttgat ttcctgaaac
      601 gggatatcat caaagccatg aacaaagcag ccgcgctgga tgaactgata ccggggttgc
      661 tgagtgaata tatcgaacag tcaggttaac aggctgcggc attttgtccg cgccgggctt
      721 cgctcactgt tcaggccgga gccacagacc gccgttgaat gggcggatgc taattactat
      781 ctcccgaaag aatccgcata ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc
      841 atcatgaatg cgatgggcag cgactacatc cgtgaggtga atgtggtgaa gtctgcccgt
      901 gtcggttatt ccaaaatgct gctgggtgtt tatgcctact ttatagagca taagcagcgc
      961 aacaccctta tctggttgcc gacggatggt gatgccgaga actttatgaa aacccacgtt
     1021 gagccgacta ttcgtgatat tccgtcgctg ctggcgctgg ccccgtggta tggcaaaaag
     1081 caccgggata acacgctcac catgaagcgt ttcactaatg ggcgtggctt ctggtgcctg
     1141 ggcggtaaag cggcaaaaaa ctaccgtgaa aagtcggtgg atgtggcggg ttatgatgaa
     1201 cttgctgctt ttgatgatga tattgaacag gaaggctctc cgacgttcct gggtgacaag
     1261 cgtattgaag gctcggtctg gccaaagtcc atccgtggct ccacgccaaa agtgagaggc
     1321 acctgtcaga ttgagcgtgc agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc
     1381 tgcccgcatt gcggggagga gcagtatctt aaatttggcg acaaagagac gccgtttggc
     1441 ctcaaatgga cgccggatga cccctccagc gtgttttatc tctgcgagca taatgcctgc
     1501 gtcatccgcc agcaggagct ggactttact gatgcccgtt atatctgcga aaagaccggg
     1561 atctggaccc gtgatggcat tctctggttt tcgtcatccg gtgaagagat tgagccacct
     1621 gacagtgtga cctttcacat ctggacagcg tacagcccgt tcaccacctg ggtgcagatt
     1681 gtcaaagact ggatgaaaac gaaaggggat acgggaaaac gtaaaacctt cgtaaacacc
     1741 acgctcggtg agacgtggga ggcgaaaatt ggcgaacgtc cggatgctga agtgatggca
     1801 gagcggaaag agcattattc agcgcccgtt cctgaccgtg tggcttacct gaccgccggt
     1861 atcgactccc agctggaccg ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa
     1921 agctggctga ttgaccggca gattattatg ggccgccacg acgatgaaca gacgctgctg
     1981 cgtgtggatg aggccatcaa taaaacctat acccgccgga atggtgcaga aatgtcgata
     2041 tcccgtatct gctgggatac tggcgggatt gacccgacca ttgtgtatga acgctcgaaa
     2101 aaacatgggc tgttccgggt gatccccatt aaaggggcat ccgtctacgg aaagccggtg
     2161 gccagcatgc cacgtaagcg aaacaaaaac ggggtttacc ttaccgaaat cggtacggat
     2221 accgcgaaag agcagattta taaccgcttc acactgacgc cggaagggga tgaaccgctt
     2281 cccggtgccg ttcacttccc gaataacccg gatatttttg atctgaccga agcgcagcag
     2341 ctgacggctg aagagcaggt cgaaaaatgg gtggatggca ggaaaaaaat actgtgggac
     2401 agcaaaaagc gacgcaatga ggcactcgac tgcttcgttt atgcgctggc ggcgctgcgc
     2461 atcagtattt cccgctggca gctggatctc agtgcgctgc tggcgagcct gcaggaagag
     2521 gatggtgcag caaccaacaa gaaaacactg gcagattacg cccgtgcctt atccggagag
     2581 gatgaatgac gcgacaggaa gaacttgccg ctgcccgtgc ggcactgcat gacctgatga
     2641 caggtaaacg ggtggcaaca gtacagaaag acggacgaag ggtggagttt acggccactt
     2701 ccgtgtctga cctgaaaaaa tatattgcag agctggaagt gcagaccggc atgacacagc
     2761 gacgcagggg acctgcagga ttttatgtat gaaaacgccc accattccca cccttctggg
     2821 gccggacggc atgacatcgc tgcgcgaata tgccggttat cacggcggtg gcagcggatt
     2881 tggagggcag ttgcggtcgt ggaacccacc gagtgaaagt gtggatgcag ccctgttgcc
     2941 caactttacc cgtggcaatg cccgcgcaga cgatctggta cgcaataacg gctatgccgc
     3001 caacgccatc cagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg
     3061 cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt
     3121 tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa
     3181 acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact
     3241 gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat
     3301 ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc
     3361 cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta
     3421 tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc
     3481 ctcgttcatt cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt
     3541 ttacagcgtg atggagcaga tgaagatgct cgacacgctg cagaacacgc agctgcagag
     3601 cgccattgtg aaggcgatgt atgccgccac cattgagagt gagctggata cgcagtcagc
     3661 gatggatttt attctgggcg cgaacagtca ggagcagcgg gaaaggctga ccggctggat
     3721 tggtgaaatt gccgcgtatt acgccgcagc gccggtccgg ctgggaggcg caaaagtacc
     3781 gcacctgatg ccgggtgact cactgaacct gcagacggct caggatacgg ataacggcta
     3841 ctccgtgttt gagcagtcac tgctgcggta tatcgctgcc gggctgggtg tctcgtatga
     3901 gcagctttcc cggaattacg cccagatgag ctactccacg gcacgggcca gtgcgaacga
     3961 gtcgtgggcg tactttatgg ggcggcgaaa attcgtcgca tcccgtcagg cgagccagat
     4021 gtttctgtgc tggctggaag aggccatcgt tcgccgcgtg gtgacgttac cttcaaaagc
     4081 gcgcttcagt tttcaggaag cccgcagtgc ctgggggaac tgcgactgga taggctccgg
     4141 tcgtatggcc atcgatggtc tgaaagaagt tcaggaagcg gtgatgctga tagaagccgg
     4201 actgagtacc tacgagaaag agtgcgcaaa acgcggtgac gactatcagg aaatttttgt
     4261 ccagcaggtc cgtgaaacga tggagcgccg tgcagccggt cttaaaccgc ccgcctgggc
     4321 ggctgcagca tttgaatccg ggctgcgaca atcaacagag gaggagaaga gtgacagcag
     4381 agctgcgtaa tctcccgcat attgccagca tggcctttaa tgagccgctg atgcttgaac
     4441 ccgcctatgc gcgggttttc ttttgtgcgc ttgcaggcca gcttgggatc agccgcctga
     4501 cggatgcggt gtccggcgac agcctgactg cccaggaggc actcgcgacg ctggcattat
     4561 ccggtgatga tgacggacca cgacaggccc gcagttatca ggtcatgaac ggcatcgccg
     4621 tgctgccggt gtccggcacg ctggtcagcc ggacgcgggc gctgcagccg tactcgggga
     4681 tgaccggtta caacggcatt atcgcccgtc tgcaacaggc tgccagcgat ccgatggtgg
     4741 acggcattct gctcgatatg gacacgcccg gcgggatggt ggcgggggca tttgactgcg
     4801 ctgacatcat cgcccgtgtg cgtgacataa aaccggtatg ggcgcttgcc aacgacatga
     4861 actgcagtgc aggtcagttg cttgccagtg ccgcctcccg gcgtctggtc acgcagaccg
     4921 cccggacagg ctccatcggc gtcatgatgg ctcacagtaa ttacggtgct gcgctggaga
     4981 aacagggtgt ggaaatcacg ctgatttaca gcggcagcca taaggtggat ggcaacccct
     5041 acagccatct tccggatgac gtccgggaga cactgcagtc ccggatggac gcaacccgcc
     5101 ggatgtttgc gcagaaggtg tcggcatata ccggcctgtc cgtgcaggct gtgctggata
     5161 ccgaggctgc agtgtacagc ggtcaggagg ccattgatgc cggactggct gatgaacttg
     5221 ttaacagcac cgatgcgatc accgtcatgc gtgatgcact ggatgcacgt aaatcccgtc
     5281 tctcaggagg gcgaatgacc aaagagactc aatcaacaac tgtttcagcc actgcttcgc
     5341 aggctgacgt tactgacgtg gtgccagcga cggagggcga gaacgccagc gcggcgcagc
     5401 cggacgtgaa cgcgcagatc accgcagcgg ttgcggcaga aaacagccgc attatgggga
     5461 tcctcaactg tgaggaggct cacggacgcg aagaacaggc acgcgtgctg gcagaaaccc
     5521 ccggtatgac cgtgaaaacg gcccgccgca ttctggccgc agcaccacag agtgcacagg
     5581 cgcgcagtga cactgcgctg gatcgtctga tgcagggggc accggcaccg ctggctgcag
     5641 gtaacccggc atctgatgcc gttaacgatt tgctgaacac accagtgtaa gggatgttta
     5701 tgacgagcaa agaaaccttt acccattacc agccgcaggg caacagtgac ccggctcata
     5761 ccgcaaccgc gcccggcgga ttgagtgcga aagcgcctgc aatgaccccg ctgatgctgg
     5821 acacctccag ccgtaagctg gttgcgtggg atggcaccac cgacggtgct gccgttggca
     5881 ttcttgcggt tgctgctgac cagaccagca ccacgctgac gttctacaag tccggcacgt
     5941 tccgttatga ggatgtgctc tggccggagg ctgccagcga cgagacgaaa aaacggaccg
     6001 cgtttgccgg aacggcaatc agcatcgttt aactttaccc ttcatcacta aaggccgcct
     6061 gtgcggcttt ttttacggga tttttttatg tcgatgtaca caaccgccca actgctggcg
     6121 gcaaatgagc agaaatttaa gtttgatccg ctgtttctgc gtctcttttt ccgtgagagc
     6181 tatcccttca ccacggagaa agtctatctc tcacaaattc cgggactggt aaacatggcg
     6241 ctgtacgttt cgccgattgt ttccggtgag gttatccgtt cccgtggcgg ctccacctct
     6301 gaatttacgc cgggatatgt caagccgaag catgaagtga atccgcagat gaccctgcgt
     6361 cgcctgccgg atgaagatcc gcagaatctg gcggacccgg cttaccgccg ccgtcgcatc
     6421 atcatgcaga acatgcgtga cgaagagctg gccattgctc aggtcgaaga gatgcaggca
     6481 gtttctgccg tgcttaaggg caaatacacc atgaccggtg aagccttcga tccggttgag
     6541 gtggatatgg gccgcagtga ggagaataac atcacgcagt ccggcggcac ggagtggagc
     6601 aagcgtgaca agtccacgta tgacccgacc gacgatatcg aagcctacgc gctgaacgcc
     6661 agcggtgtgg tgaatatcat cgtgttcgat ccgaaaggct gggcgctgtt ccgttccttc
     6721 aaagccgtca aggagaagct ggatacccgt cgtggctcta attccgagct ggagacagcg
     6781 gtgaaagacc taggtaaagc ggtgtcctat aaggggatgt atggcgatgt ggccatcgtc
     6841 gtgtattccg gacagtacgt ggaaaacggc gtcaaaaaga acttcctgcc ggacaacacg
     6901 atggtgctgg ggaacactca ggcacgcggt ctgcgcacct atggctgcat tcaggatgcg
     6961 gacgcacagc gcgaaggcat taacgcctct gcccgttacc cgaaaaactg ggtgaccacc
     7021 ggcgatccgg cgcgtgagtt caccatgatt cagtcagcac cgctgatgct gctggctgac
     7081 cctgatgagt tcgtgtccgt acaactggcg taatcatggc ccttcggggc cattgtttct
     7141 ctgtggagga gtccatgacg aaagatgaac tgattgcccg tctccgctcg ctgggtgaac
     7201 aactgaaccg tgatgtcagc ctgacgggga cgaaagaaga actggcgctc cgtgtggcag
     7261 agctggaaga ggagcttgat gacacggatg aaactgccgg tcaggacacc cctctcagcc
     7321 gggaaaatgt gctgaccgga catgaaaatg aggtgggatc agcgcagccg gataccgtga
     7381 ttctggatac gtctgaactg gtcacggtcg tggcactggt gaagctgcat actgatgcac
     7441 ttcacgccac gcgggatgaa cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct
     7501 ctgccggtgt ggcagccgaa atgacagagc gcggcctggc cagaatgcaa taacgggagg
     7561 cgctgtggct gatttcgata acctgttcga tgctgccatt gcccgcgccg atgaaacgat
     7621 acgcgggtac atgggaacgt cagccaccat tacatccggt gagcagtcag gtgcggtgat
     7681 acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga
     7741 aggctccagc ccgtccctgt ttgtccggac tgatgaggtg cggcagctgc ggcgtggaga
     7801 cacgctgacc atcggtgagg aaaatttctg ggtagatcgg gtttcgccgg atgatggcgg
     7861 aagttgtcat ctctggcttg gacggggcgt accgcctgcc gttaaccgtc gccgctgaaa
     7921 gggggatgta tggccataaa aggtcttgag caggccgttg aaaacctcag ccgtatcagc
     7981 aaaacggcgg tgcctggtgc cgccgcaatg gccattaacc gcgttgcttc atccgcgata
     8041 tcgcagtcgg cgtcacaggt tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa
     8101 agggccaggc tgaaaagggc cacggtcaaa aatccgcagg ccagaatcaa agttaaccgg
     8161 ggggatttgc ccgtaatcaa gctgggtaat gcgcgggttg tcctttcgcg ccgcaggcgt
     8221 cgtaaaaagg ggcagcgttc atccctgaaa ggtggcggca gcgtgcttgt ggtgggtaac
     8281 cgtcgtattc ccggcgcgtt tattcagcaa ctgaaaaatg gccggtggca tgtcatgcag
     8341 cgtgtggccg ggaaaaaccg ttaccccatt gatgtggtga aaatcccgat ggcggtgccg
     8401 ctgaccacgg cgtttaaaca aaatattgag cggatacggc gtgaacgtct tccgaaagag
     8461 ctgggctatg cgctgcagca tcaactgagg atggtaataa agcgatgaaa catactgaac
     8521 tccgtgcagc cgtactggat gcactggaga agcatgacac cggggcgacg ttttttgatg
     8581 gtcgccccgc tgtttttgat gaggcggatt ttccggcagt tgccgtttat ctcaccggcg
     8641 ctgaatacac gggcgaagag ctggacagcg atacctggca ggcggagctg catatcgaag
     8701 ttttcctgcc tgctcaggtg ccggattcag agctggatgc gtggatggag tcccggattt
     8761 atccggtgat gagcgatatc ccggcactgt cagatttgat caccagtatg gtggccagtg
     8821 gctatgacta ccggcgcgac gatgatgcgg gcctgtggag ttcagccgat ctgacttatg
     8881 tcattaccta tgaaatgtga ggacgctatg cctgtaccaa atcctacaat gccggtgaaa
     8941 ggtgccggga ccaccctgtg ggtttataag gggagcggtg acccttatgc gaatccgctt
     9001 tcagacgttg actggtcgcg tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc
     9061 gctgagtcct atgacgacag ctatctcgat gatgaagatg cagactggac tgcgaccggg
     9121 caggggcaga aatctgccgg agataccagc ttcacgctgg cgtggatgcc cggagagcag
     9181 gggcagcagg cgctgctggc gtggtttaat gaaggcgata cccgtgccta taaaatccgc
     9241 ttcccgaacg gcacggtcga tgtgttccgt ggctgggtca gcagtatcgg taaggcggtg
     9301 acggcgaagg aagtgatcac ccgcacggtg aaagtcacca atgtgggacg tccgtcgatg
     9361 gcagaagatc gcagcacggt aacagcggca accggcatga ccgtgacgcc ttccagcacc
     9421 tcggtggtga aagggcagag caccacgctg accgtggcct tccagccgga gggcgtaacc
     9481 gacaagagct ttcgtgcggt gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt
     9541 atgaccatca ccgtgaacgg cgttgctgca ggcaaggtca acattccggt tgtatccggt
     9601 aatggtgagt ttgctgcggt tgcagaaatt accgtcaccg ccagttaatc cggagagtca
     9661 gcgatgttcc tgaaaaccga atcatttgaa cataacggtg tgaccgtcac gctttctgaa
     9721 ctgtcagccc tgcagcgcat tgagcatctc gccctgatga aacggcaggc agaacaggcg
     9781 gagtcagaca gcaaccggaa gtttactgtg gaagacgcca tcagaaccgg cgcgtttctg
     9841 gtggcgatgt ccctgtggca taaccatccg cagaagacgc agatgccgtc catgaatgaa
     9901 gccgttaaac agattgagca ggaagtgctt accacctggc ccacggaggc aatttctcat
     9961 gctgaaaacg tggtgtaccg gctgtctggt atgtatgagt ttgtggtgaa taatgcccct
    10021 gaacagacag aggacgccgg gcccgcagag cctgtttctg cgggaaagtg ttcgacggtg
    10081 agctgagttt tgccctgaaa ctggcgcgtg agatggggcg acccgactgg cgtgccatgc
    10141 ttgccgggat gtcatccacg gagtatgccg actggcaccg cttttacagt acccattatt
    10201 ttcatgatgt tctgctggat atgcactttt ccgggctgac gtacaccgtg ctcagcctgt
    10261 ttttcagcga tccggatatg catccgctgg atttcagtct gctgaaccgg cgcgaggctg
    10321 acgaagagcc tgaagatgat gtgctgatgc agaaagcggc agggcttgcc ggaggtgtcc
    10381 gctttggccc ggacgggaat gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg
    10441 aggatgacgt aatgctgatg acagtatcag aagggatcgc aggaggagtc cggtatggct
    10501 gaaccggtag gcgatctggt cgttgatttg agtctggatg cggccagatt tgacgagcag
    10561 atggccagag tcaggcgtca tttttccggt acggaaagtg atgcgaaaaa aacagcggca
    10621 gtcgttgaac agtcgctgag ccgacaggcg ctggctgcac agaaagcggg gatttccgtc
    10681 gggcagtata aagccgccat gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag
    10741 cttgcaggcg ggcaaagtcc gtggctgatc ctgctgcaac aggggggcca cctcgctggc
    10801 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    10861 caacaaaacg ctggtccttt ccggcaatca ggcgggactg acggcagatc gtatgctggt
    10921 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
    10981 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    11041 ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    11101 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
    11161 gacggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
    11221 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
    11281 gaacatgggc acgctggaga cctgggcaga caggactgcg cgggcattca aatccatgtg
    11341 ggatgcggtg ctggatatcg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
    11401 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt attttgttaa
    11461 cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc
    11521 cgcccgaaag aaggctgagc agcagactca acaggacaaa aatgcgcagc agcagagcga
    11581 taccgaagcg tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca
    11641 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    11701 gaaaatcctg caggcggatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    11761 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtct gcgggcgatc gtcaggaaga
    11821 cagtgctcat gctgccctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc
    11881 cggagcaaat gagaaaatca gccagcagcg ccgggatttg tggaaggcgg agagtcagtt
    11941 cgcggtactg gaggaggcgg cgcaacgtcg ccagctgtct gcacaggaga aatccctgct
    12001 ggcgcataaa gatgagacgc tggagtacaa acgccagctg gctgcacttg gcgacaaggt
    12061 tacgtatcag gagcgcctga acgcgctggc gcagcaggcg gataaattcg cacagcagca
    12121 acgggcaaaa cgggccgcca ttgatgcgaa aagccggggg ctgactgacc ggcaggcaga
    12181 acgggaagcc acggaacagc gcctgaagga acagtatggc gataatccgc tggcgctgaa
    12241 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggaactg
    12301 gatggcaggc ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    12361 gcaggtaaaa agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcggcgat
    12421 gctgaccggc agtgagcaga actggcgcag cttcacccgt tccgtgctgt ccatgatgac
    12481 agaaattctg cttaagcagg caatggtggg attgtcggga gtatcggcag cgccattggc
    12541 ggggctgttg gtggcggcgc atccgcgtca ggcggtacag ccattcaggc cgctgcggcg
    12601 aaattccatt ttgcaaccgg aggatttacg ggaaccggcg gcaaatatga gccagcgggg
    12661 attgttcacc gtggtgagtt tgtcttcacg aaggaggcaa ccagccggat tggcgtgggg
    12721 aatctttacc ggctgatgcg cggctatgcc accggcggtt atgtcggtac accgggcagc
    12781 atggcagaca gccggtcgca ggcgtccggg a
//
LOCUS       DAZFWP020000139        12588 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFWP020000139 DAZFWP020000000
VERSION     DAZFWP020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.139"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            361..1749
                     /gene="narU"
                     /locus_tag="ACHK0Z_RS19230"
                     /old_locus_tag="pgaptmp_003846"
     CDS             361..1749
                     /gene="narU"
                     /locus_tag="ACHK0Z_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU4503794.1"
                     /translation="MALQNEKNNRYLLRDWKPENPTFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1831..5571
                     /gene="narZ"
                     /locus_tag="ACHK0Z_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             1831..5571
                     /gene="narZ"
                     /locus_tag="ACHK0Z_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU4503795.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSASQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5568..7112
                     /gene="narH"
                     /locus_tag="ACHK0Z_RS19240"
                     /old_locus_tag="pgaptmp_003848"
     CDS             5568..7112
                     /gene="narH"
                     /locus_tag="ACHK0Z_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4503796.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7112..7807
                     /gene="narW"
                     /locus_tag="ACHK0Z_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             7112..7807
                     /gene="narW"
                     /locus_tag="ACHK0Z_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4503797.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7804..8484
                     /gene="narI"
                     /locus_tag="ACHK0Z_RS19250"
                     /old_locus_tag="pgaptmp_003850"
     CDS             7804..8484
                     /gene="narI"
                     /locus_tag="ACHK0Z_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4503798.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8563..9456
                     /locus_tag="ACHK0Z_RS19255"
                     /old_locus_tag="pgaptmp_003851"
     CDS             8563..9456
                     /locus_tag="ACHK0Z_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU4503799.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9552..10397)
                     /gene="nhoA"
                     /locus_tag="ACHK0Z_RS19260"
                     /old_locus_tag="pgaptmp_003852"
     CDS             complement(9552..10397)
                     /gene="nhoA"
                     /locus_tag="ACHK0Z_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGU4503800.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDGWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10570..11139
                     /locus_tag="ACHK0Z_RS19265"
                     /old_locus_tag="pgaptmp_003853"
     CDS             10570..11139
                     /locus_tag="ACHK0Z_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU4503801.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDNKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11143..11370)
                     /gene="pptA"
                     /locus_tag="ACHK0Z_RS19270"
                     /old_locus_tag="pgaptmp_003854"
     CDS             complement(11143..11370)
                     /gene="pptA"
                     /locus_tag="ACHK0Z_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU4503802.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11469..12587)
                     /locus_tag="ACHK0Z_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /pseudo
     CDS             complement(11469..12587)
                     /locus_tag="ACHK0Z_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2996 a         3249 c         3218 g         3125 t
ORIGIN      
        1 ggacagatcg ttttcctcca gttcatggcc tttttagtga ataaatcatc actaattcta
       61 taagtgtctg gtcttgttga taatatagtt aatatgtaac cctttagatt cacgatctga
      121 agggttgaaa catgacatac gttcaaagat taacccccgt ttgaaggtta gcttgttagt
      181 tacatttagt aacacatatc tggctactta atccccattc tgcccccgcg gttacgcttt
      241 cttatctatt ctttcaatca gcacttcacc caattcatcc ttaattctaa taactggcta
      301 ttgcgcctta cctcctgttt tgtctttttt cattaccaat gtgtgcatgt gaggaacaat
      361 atggcactgc aaaatgagaa aaataatcgt tatcttttac gcgactggaa accagaaaat
      421 ccgaccttct gggaaaataa aggaaaacat attgctcgaa gaaacctctg gatatcagtc
      481 agttgtctac ttcttgcctt ctgtgtctgg atgctattta gcgcagttac cgttaatctc
      541 aataaaatcg gttttaattt tactaccgat caactctttt tattaaccgc attaccctcc
      601 gtttctggcg cattattgcg tgttccctac tcctttatgg tgcctatatt cggtggacgc
      661 cgatggacgg tttttagtac tgcaatcctg attattcctt gcgtctggct cggaattgcc
      721 gtgcaaaatc cgaatactcc ttttgggata tttatcgtta tcgctttgct atgcggtttt
      781 gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc aaaagccaaa
      841 caagggagcg ctcttgggat taatggcgga ttaggaaact taggtgtaag tgtaatgcag
      901 ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt caatggcgta
      961 ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg ggtaccgcta
     1021 ctggcgattg ccacgatcgc cgcctggtca gggatgaatg atatcgccag ttcacgcgcc
     1081 tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct gagcctgctt
     1141 taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat gctggcaaaa
     1201 acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt tatcggtgcc
     1261 atcgcgcggt cggttggtgg tgctatttcc gataagttcg gcggcgtgcg ggtgacgttg
     1321 atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt accgggcaca
     1381 ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct gactgcgggt
     1441 ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat aaccatttat
     1501 cgggtaaaga tgaaaggcgg tagtgatgag caagctcaga aagaagccgt caccgaaacg
     1561 gcggcggctc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt tattccgcag
     1621 gcgtttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa agtgttttta
     1681 atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg gaagttcagc
     1741 caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag gcatcagatc
     1801 ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg ctttcgctac
     1861 ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca tagcaaccgc
     1921 gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg ttccacccac
     1981 ggtgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg tctggtgacc
     2041 tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa tcatgaacct
     2101 cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa ccgcctgaaa
     2161 tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagccctcaa acaacacagc
     2221 gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgcct gagctacaaa
     2281 caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc aggaactaaa ccagctgatt
     2341 gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc cggtttctcg
     2401 ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct gtcgctgctt
     2461 ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc ctcgccgatg
     2521 acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc cagctacatc
     2581 atcgcctggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt ctttaccgaa
     2641 gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga agtggccaaa
     2701 ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc gatggcaatg
     2761 ggccatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt tatcaactac
     2821 tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga cgatggtagc
     2881 tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg cgaaagcaac
     2941 aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt gccgaacggt
     3001 tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat tgccgccggt
     3061 acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc aggcgtggcc
     3121 ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca caatccggtg
     3181 ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac ctgtccggtg
     3241 gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct ggaagatgaa
     3301 aacagtgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg tgagcaaatt
     3361 accggcgtgc cgcgccagta tattgaaacc atcgcccgtg aatttgccga taccgcccat
     3421 aaaacgcatg ggcgctcgat gattatcctc ggcgcaggtg ttaaccactg gtatcacatg
     3481 gatatgaact accgtgggat gatcaatatg ctgatcttct gcggttgtgt cgggcaaagc
     3541 ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac cggctggttg
     3601 ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc aaatgaacag cacctcgttt
     3661 ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga gttactttca
     3721 ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt tcgcgccgaa
     3781 cgtatgggct ggctaccttc tgcgtcgcag ctggggcgta acccgctcgg gattaaagct
     3841 gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct gaaatcgggc
     3901 gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg taatttgttt
     3961 gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat gcagaagtat
     4021 ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga cgggatcaaa
     4081 ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct gctggtgacg
     4141 ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc caccgccacc
     4201 tggtacgaaa aagacgatat gaacacctcg gatatgcatc cgtttattca tccgctttct
     4261 gcggcggtcg atccggcgtg ggaatcacgc agcgactggg aaatctacaa aggtatcgcc
     4321 aaagcatttt cgcaagtgtg cgtgggtcat cttggcaaag aaaccgacgt ggtattacaa
     4381 cccctactgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct cgactggcgc
     4441 aaaggcgaat gcgatctgat tccaggcaaa accgcgccga atattgtggc ggtggagcgc
     4501 gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga caaacttggc
     4561 aatggcggta aagggatttc gtggaatacg caggatgaaa tcgatttcct cggcaaactc
     4621 aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac cgccattgac
     4681 gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc agtcaaagcg
     4741 tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct gcacaaagag
     4801 gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat ctccagcccc
     4861 acctggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac caacgttcat
     4921 gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga tcatccgtgg
     4981 atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac ccgtagcgtc
     5041 agtgagatgc gccagatacc gccaaacggc ttcccggaaa aagcacttaa cttcctgacg
     5101 ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat gctgacgctc
     5161 tctcgcggtg gaccgattgt ctggatcagc gaaacagatg cccgagaact gaccattgtc
     5221 gataacgact gggtggaagt attcaacgcc aacggcgcgc tgactgcccg cgcggtggtc
     5281 agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg cattatgaat
     5341 attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt tacccgcgtt
     5401 tgcccgaaac cgacgcatat gattggcggt tacgcgcagc tggcctgggg ctttaactac
     5461 tacggcaccg tcggatcgaa ccgcgatgag ttcatcatga tccgcaagat gaagaacgtt
     5521 aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg aaaatccgtt
     5581 cacaagtcgg catggtgctt aacctcgaca aatgtatcgg ctgccatacc tgttcggtga
     5641 cctgtaaaaa cgtctggacc gggcgcgaag gcatggagta cgcatggttt aacaacgtcg
     5701 aaaccaaacc gggcattggt tatccgaaaa actgggaaga tcaggaagag tggcaaggcg
     5761 gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct gggcagcaag atgggcgtaa
     5821 taaccaaaat cttcgccaac ccggtggtgc cgcagattga tgattactac gaacctttca
     5881 ctttcgacta cgaacatttg catagcgcac cggaaggcaa acatatccct actgctcgcc
     5941 cgcgctcgct gattgacggt aagcggatgg acaaagtgat ctgggggcca aactgggaag
     6001 aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag gccatacaaa
     6061 aggagatgta cgggcagttt gaaaacacct tcatgatgta cctgccgcgc ctgtgcgaac
     6121 actgcctcaa tcccagttgc gtggcgacct gcccaagcgg cgctatctac aaacgcgaag
     6181 aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg tgcattagcg
     6241 gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa aaatgtatct
     6301 tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc tgcgtgggtc
     6361 gcatccggta tctgggcgtg ctgctttacg acgccgaccg catcgaggaa gcggcgagca
     6421 ccgagcgcga agttgacctc tatgaacgtc agtgcgaagt gttcctcgat ccacacgatc
     6481 cctcagtgat cgaggaagcc ctgaaacaag gtattccaca aaacgtgatt gaagctgccc
     6541 agcgttcgcc tgtctacaaa atggcgatgg actggaaact ggcgctaccg ctgcaccctg
     6601 aatatcgcac cctgccgatg gtctggtacg ttcctccgct gtcgccgatt cagtcctacg
     6661 cagatgcggg cggtttgccg aaaagcgaag gcgtgctgcc cgccatcgaa agcctgcgta
     6721 ttccggtgca atatctcgcc aatatgttga gtgccggcga taccggtccg gtactgcggg
     6781 cgctgaaacg gatgatggcg atgcgccact atatgcgttc acaaaccgtg gaaggcgtta
     6841 ctgatactcg tgccatcgac gaagtaggcc tgagcgtcgc ccaggtcgaa gagatgtatc
     6901 gttacctcgc cattgccaac tatgaagatc gttttgtcat cccgacgagc catcgggaaa
     6961 tggcgggcga tgccttcgca gaacgcaacg gctgcggttt tacctttggc gacggttgcc
     7021 acggttcgga cagtaaattc aacctgttca acagtagccg tatcgatgcc atcaacatca
     7081 ccgaagtgcg cgacaaagcg gagggcgaat aatgcagatc ctcaaagtga tcggcctgtt
     7141 gatggagtat ccggacgagc tgttgtggga atgcaaggag gacgcgctgg cgttgatccg
     7201 cagcgacgcg ccgatgctta cggatttcac ccacaacctg cttaacgcgc cgctgctgga
     7261 taaacaggcc gaatggtgcg aagtgtttga ccgtggacgt accacatcgc tgctgctgtt
     7321 cgaacatgtt catgccgagt cccgcgatcg cggccaggca atggtggacc tgctggcgga
     7381 gtatgaaaag gtcggcctgc agctggattg tcgggaactg cccgattatc taccgctgta
     7441 tctggagtat ttaagcgtac tgcctgacga tcaggcgaaa gaaggattgc tcaatgttgc
     7501 gccgatcctc gccctgcttg gcggtcgctt aaaacaacgc gaggcaccgt ggtacgcgtt
     7561 gtttgatgct ctgctgcaac tggcgggaag ctccctttca agtgacagtg tcacaaaaca
     7621 agtaaacagc gaagagcgcg atgacacccg ccaggcgctt gatgcagtgt gggaagagga
     7681 acaggtgaag tttattgaag ataacgccac ggcgtgtgac agctcgccgc ttaatcaata
     7741 tcagcgacgc tttagccagg atgtcgcgcc gcaatatgtc gacatcagtg cgggaggtgg
     7801 gaaatgattc agtatctgaa cgtctttttt tacgatatct acccgtacat ttgtgcgacg
     7861 gtgtttttcc tcggcagctg gctgcgctac gactacgggc agtacacctg gcgcgcctcc
     7921 tcaagtcaaa tgctcgataa acgcgggatg gttatatggt cgaatctgtt ccatatcggc
     7981 attttgggga tcttcttcgg gcacctgttc ggcatgttaa cgccgcactg gatgtacgcg
     8041 tggtttttgc cagtggcagc gaaacaacta atggcgatgg tgctcggcgg tatttgcggt
     8101 gtgttgacat tgattggcgg cgcaggactg ctgtggcgca ggctgacaaa tcagcgcgtg
     8161 cgtgcaactt ccactacgcc ggatattatc atcatgagca ttttgctgat ccagtgtctg
     8221 ctggggctaa gtaccatacc gttttcagca cagtatcctg atggtagcga aatgatgaag
     8281 ctggtgggtt gggcgcaaag cattgtcact ttccgtggtg gatcgtcaga aatgcttaac
     8341 ggcgtagcgt tcgttttccg cctgcatctg gtattgggaa tgaccatctt cctgctcttc
     8401 ccgttcacca gattggtgca tgtgtggagc gcgccgtttg agtactttac tcgtcggtat
     8461 cagattgtgc gttcgcggcg ataatttcac cctggccgga tagcccttcg ctctccggcc
     8521 acttgcaggc tatgatagtt ttccagtcac aggagaacgt taatgaaacc gcaggtgtac
     8581 cacgtcgatg cttttacctc tcaaccattt cgcggcaatt ctgccggtgt tgttttcccc
     8641 gccgataatc ttagcgaagc gcaaatgcag cttatcgccc gcgagttagg ccattcggaa
     8701 actgcttttc tgctgcacag cgacgacagc gatgtgcgca tccgctactt tacgccaacg
     8761 gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc actatgtacg tgcgaaggtg
     8821 ttaggtttag gaaattgcac gatctggcaa acatcgctgg caggaaaaca tcgcgtgact
     8881 atcgaaaagc acaacgatga ttatcgtatt tcgctggaac aaggtacgcc gggctttgag
     8941 ccaccgctgg aaggtgaaac acgtgaggcg attatcaacg cgcttcatct cactgaagac
     9001 gatattttgc cgggcttgcc aattcaggtc gcaacaacag gacactcaaa agtgatgatc
     9061 ccgttgaaac cggaagtgga tatcgacgcc ctttcgcctg atcttgctgc gctgagcgct
     9121 atcagcaaac agattggctg caatggtttc ttcccgttcc agatccgccc aggcaaaaac
     9181 gaaaccgatg gtcgtatgtt ctcgcctgcg attggtattg tggaagatcc ggtgactggc
     9241 aatgccaatg gcccgatggg tgcatggttg gtacatcaca acgtattgcc ccacgatggc
     9301 aacgtgttat gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg catgattgaa
     9361 gtgacggtga caattcgtga taaccaaccg gaaaaagtca ccatttcagg cacagcggtg
     9421 attttgtttc atgccgaatg ggcaattgaa ctctgaaacc gttgcctgat gcgcttcgcc
     9481 tgtcaggtct acaaccgagc taatgtatgc cggataaggc gtttacgccg catccggcaa
     9541 cctgacataa attattttcc cgcctccgga tgcgtatcaa acgccgccat caccagcgct
     9601 aactcatcca cagtaaagcc atgtttcgca tcatccacgc ccagaccaaa ctgttcctgc
     9661 atcacggcat ataacgacac cacatccggt agatttcgct gctccaccgc gtgcccattt
     9721 tcataatggg taaaatgaaa attggtcagc gttagcttgc caccgtccgg caaatggcgg
     9781 cacatcagca aatgatggcg aaaatgcgac tgcggccagt gcgccgacca gaaattgccc
     9841 atcacataat cgctttgttg ctgctcgcag agatcaaaac ggtacatcga ctgccaatgc
     9901 tgatgatgat taaactgcaa cacccaacca tcaccctcct gcaacaaccg atactctccg
     9961 tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg gcgcggttag cgtctgccca
    10021 ccgaaaccga catcagcaat ccatttttcc tcttccagtt ccaccaacag caaacgatgg
    10081 gtgcgcggcg gtaatgctgg cggatttgat aacactacgc gccctaacaa gctgcgaacg
    10141 ttaaacccca gctcgcgtaa cacccgctca aacacgccat tctgctcaaa acagtaaccg
    10201 ccacgacggg ctatcaccag tttctcttcc agcgattgat catcaagctg tatttccctc
    10261 ggcagcaaaa cgtcaaggtt ttcaaacgga atagtgcaat tgtgtttcag gtgcaatgca
    10321 cgaagcgtat caatattgac cgcagcagct cccgaccagt taatacgggc aaaatagtga
    10381 ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt ctcaacttat aacctggaat
    10441 agacgccgca ccggctattt tgtgcgatcg cggacatgca tcacgtttgc aaatcgccaa
    10501 tcccgttaat ttcgttactt attgcgttac ggactacgct taaaaaaacc ttcttcgcga
    10561 ggcgatacca tgagccgatt catccccata gaattacacc atgccagccg tctgttaaat
    10621 catggcccaa ctgtcatgat caccagtttc gatgagcaat cccagcggcg taacattatg
    10681 gcagcagcct ggtcaatgcc ggtggagttt gaaccgccac gcgtggcgat tgtggtagat
    10741 aaatcgacat ggaccaggga gttgattgaa cgtaacggta aatttggcat cgttatcccg
    10801 ggcgttgcag caactaactg gacgtgggca gtgggaagcg tgtcgggtcg tgaagaagat
    10861 aaatttaatt gctatggcat tccggtggtg aaaggtccgg tgcttggttt gccacttgtt
    10921 gaagagaaat gcctggcgtg gatggagtgc cgcctgctac ctgcgacttc tgcgcaacaa
    10981 aaatacgaca cgctgtttgg cgaggtggta tcagcagcgg cagacgcacg ggtatttgtc
    11041 gaaggccgct ggcagtttga tgataataag ctcaatacgt tgcatcattt aggtgctggc
    11101 acgtttgtta ctagcggcaa gcgtgttacg gcaggttaag cattacatgc tgtaaccggg
    11161 tttctttatc aaagcctcca tttgaggcgc gatttcggca tcccagatag cttgccaaga
    11221 ttctggttga atctgctgta gagcaatgct tatcgaactg tctttactgt tcagatggcg
    11281 aataataacg tcggtaatat ctgcagcaag tgctgctttt tgttgttcgt ccagttcacg
    11341 cggaaagcat ttaatgtcga tgtgcggcat gattacgctc cttttgaaag atgtcagtaa
    11401 aattaacaca cattgtgaat gtcatgtgtc cggtaaagcg tttatacgac aataatgcca
    11461 gggcaagatt acgaaagcct tctccccgca aggactgacg ccaggtagtt tctgtccatg
    11521 gctgcttttc gcatcttacg tcttaaccct gccttgaata ccttatcatt cgtcaaaata
    11581 ttaatagcaa tgtgccgtat ccctgaaaat aattctgctg catttccttt tcttattttg
    11641 cagtcgtctt cattcattac cacgtccaga cgccagtgca gcttattctc cacgtgccag
    11701 tggtttcgga ttgctgtggc gaacttctct gcggttaaat cagcagaact gatataatat
    11761 ctgaccgtca tttctggctc tttcttttgt tctgctatta tggaccgaaa ggagactgcc
    11821 acgcataatt tcttcagccc tttccattca aacgtgaaat caataagttc atcagggaca
    11881 tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac tcttttcact cattgcgtaa
    11941 ctgtcatgct ctggattatt taattctttc agcggaaatt tttcctcaaa ggctttattt
    12001 agccgcccct ggtttccttt taccgcgaat aaataatcac ctccctgttt ttgtatcttt
    12061 tctgcaatat ctttctggca gcccatcgca tcagttgtga tgatttttcc tttaatatcc
    12121 atcatgttaa gaagttcagg aatggctgta atctcattgg atttctcatc cgtcttgatc
    12181 tgtccgagga ccagactatg cattgttgag aacgcactaa tgacatgaat cgctcccctg
    12241 cgacgactct tgtcataaga gtgccggagc gtttttccat caattgcaat gacgtcttta
    12301 tcatctgaag aatggcagtc acgcatccag ttaataaagc actcgtgaaa ttttgcagga
    12361 ctgatacagg atacaactct ggcaatgata tcgtgaaaag gaataccatt ttcaaaatca
    12421 ccatatcact tcaaaacatc gggatgtgtt tccccaaaat cctctatatc ttcccagcct
    12481 tctgcaccag aaataacggc acaaatagtc aacagtagaa tatccgataa tttatgttcc
    12541 actttccagg cttgtctgta atcggggata atagaaatat gttccatc
//
LOCUS       DAZFWP020000140        12320 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFWP020000140 DAZFWP020000000
VERSION     DAZFWP020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.140"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(84..236)
                     /locus_tag="ACHK0Z_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             complement(84..236)
                     /locus_tag="ACHK0Z_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4503803.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(496..1230)
                     /gene="cysH"
                     /locus_tag="ACHK0Z_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             complement(496..1230)
                     /gene="cysH"
                     /locus_tag="ACHK0Z_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4503804.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHK0Z_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHK0Z_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU4503805.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHK0Z_RS19295"
                     /old_locus_tag="pgaptmp_003859"
     CDS             complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHK0Z_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU4503806.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5134..5496
                     /gene="queD"
                     /locus_tag="ACHK0Z_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             5134..5496
                     /gene="queD"
                     /locus_tag="ACHK0Z_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4503807.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5574..6845
                     /gene="ygcN"
                     /locus_tag="ACHK0Z_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             5574..6845
                     /gene="ygcN"
                     /locus_tag="ACHK0Z_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4503808.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6836..7096
                     /gene="ygcO"
                     /locus_tag="ACHK0Z_RS19310"
                     /old_locus_tag="pgaptmp_003862"
     CDS             6836..7096
                     /gene="ygcO"
                     /locus_tag="ACHK0Z_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4503809.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7113..7688
                     /gene="ygcP"
                     /locus_tag="ACHK0Z_RS19315"
                     /old_locus_tag="pgaptmp_003863"
     CDS             7113..7688
                     /gene="ygcP"
                     /locus_tag="ACHK0Z_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU4503810.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7836..8696)
                     /gene="ygcQ"
                     /locus_tag="ACHK0Z_RS19320"
                     /old_locus_tag="pgaptmp_003864"
     CDS             complement(7836..8696)
                     /gene="ygcQ"
                     /locus_tag="ACHK0Z_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4503811.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8693..9472)
                     /gene="ygcR"
                     /locus_tag="ACHK0Z_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     CDS             complement(8693..9472)
                     /gene="ygcR"
                     /locus_tag="ACHK0Z_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU4503812.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9450..10859)
                     /gene="ygcS"
                     /locus_tag="ACHK0Z_RS19330"
                     /old_locus_tag="pgaptmp_003866"
     CDS             complement(9450..10859)
                     /gene="ygcS"
                     /locus_tag="ACHK0Z_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4503813.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGISLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10881..>12320)
                     /gene="ygcU"
                     /locus_tag="ACHK0Z_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             complement(10881..>12320)
                     /gene="ygcU"
                     /locus_tag="ACHK0Z_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU4503814.1"
                     /translation="AAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLP
                     IPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQ
                     IINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRSIGQFS
                     TLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITEVTVKI
                     FKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHFADGKC
                     VLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQ
                     ILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNM
                     YFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHG
                     SAWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         2814 a         3396 c         3156 g         2954 t
ORIGIN      
        1 ataaaaaata taaattttaa aaaagggtgc aggagaccaa accatcacaa ttcagtacgc
       61 ggggttttac ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac tgccttaaac
      121 tccataccac gttctctgat actcaactca cacaacgagt cacctaccat cagcgtgaat
      181 cccagtactg tacaacacag tacgatgatt gccacaaggg catattttgt cagcatgtct
      241 ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc ctcaggttga
      301 tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca
      361 gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt tcacgtttaa
      421 aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa
      481 tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc caaagaaacg
      541 tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat cgcccaccga
      601 taaatatcct tcgtcccata atgggtgata tttcaggcca tgtttttgca gatactgata
      661 tatcgttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc
      721 cagcaccggt aaattggcgc ggctgccgga ttgttcgcgg cgcaggccag caaaccacgt
      781 ttgcgcattc agttctttca gcgcccggtt catcggttcg actttgttga tttcattgta
      841 cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt cctgccaggc
      901 ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg ttaactcgtc
      961 aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca ccggaatatc
     1021 cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa agctggaaga
     1081 aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt cagcgtccag
     1141 tttttccagt tcggcgttag tttctgccag cgccagaatg cgatctacct tcggcagttc
     1201 gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc
     1261 gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc
     1321 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
     1381 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
     1441 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc
     1501 caagatgcag attgtagcga cccggtgctt tacccaccag gcccacttcc gccagcatcg
     1561 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
     1621 catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctctgctt
     1681 ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc catcgagttt tcacgctgcg
     1741 gcgtgacggc attcattaaa ccgctctctt tggcgatctt ctcgatcttc gctttctcgc
     1801 tttccggtac accggcgatg atcagattct ggttcgccgt aatgcggaaa tcgcctttgt
     1861 ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa tcaaggatgc
     1921 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
     1981 tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg atccccgcgc
     2041 gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg tatttggttt
     2101 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg acggcttccg
     2161 ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg cgggcgtagg
     2221 ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg aagcctacca
     2281 gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga tcgatatcgt
     2341 tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc tgaccaagga
     2401 tcggttcttc atcagtagtg gcgacttttt cctggtcgag ccagatctcc gcatacgcgc
     2461 gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct tccgcgtgca
     2521 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttagctgtcg
     2581 ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca
     2641 gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
     2701 tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc ccacccggca
     2761 gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc
     2821 ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttatcgc
     2881 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
     2941 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
     3001 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
     3061 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
     3121 tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc
     3181 atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt gtacgtaaac
     3241 tttttctttt tggtcgcgcg accaggcaag atcgatacgt gtcagcacgc catctttgac
     3301 gtagcgctgc cactccacct ggtacaggaa gtcttccgta aagtgcggat taccaaagaa
     3361 cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc tgcataaagg cgcggaacgg
     3421 cgcaataccg gtgcctgggc caatcataat caccggggtt tccgggttag cgggcaggcg
     3481 aaagttatcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca cgcgatccgc
     3541 gaggaagctg gaggcaccac cggcacgggc tcggccttcc acgtcgtaac gcaccacacc
     3601 aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg aatacaggcg
     3661 cggggtcagc gggcgcagca gattaatcag cgcttcggca tccagttgcg ccggagagaa
     3721 acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
     3781 caccagcggc agcagtgttt cactgcgggt aagcgtggcg taattctcaa caatgttggc
     3841 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
     3901 ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt ctttcaccag
     3961 tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac gcaggcccga
     4021 gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgtc cggtaatttt
     4081 ctggttaacc gaaaggctcg ccaccagcgg agcgtctttg ctgtacgggc tggagtggat
     4141 ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
     4201 aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt caacatcggc
     4261 atcgacacgg tcgagcaggc gttcaccgcc cagttccgcc agcttgctgt caaaatcttt
     4321 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaatgcggt
     4381 gttttccagc ttcggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
     4441 cggcggttcc ccttctcctt gcgttgacgt caccacgata agcagttttt cgctggcgat
     4501 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ctgctaataa
     4561 atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgagg cggagataat
     4621 agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag caggctgctg
     4681 attgagtacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag ttaaatcggt
     4741 cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg
     4801 gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
     4861 gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg attaaagacg
     4921 ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca
     4981 aaatagatta accaacctaa tgaaaaacaa atgaatttag ccaatcatta agataaatca
     5041 gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct
     5101 acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt taaagatttc
     5161 accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg tggtcgcctg
     5221 cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc
     5281 tggattatcg atttcgctga actaaaagcg gcgtttaagc caacttatga gcgcctcgat
     5341 caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaagt tttagcaaaa
     5401 tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa
     5461 acctgcaccg ctggttgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt
     5521 cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat gacatggaag
     5581 acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc gcgttacgct
     5641 gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa
     5701 atctttccgg cgggcggtta tatacccatg cactcgcgga actcctcccg caatttcatc
     5761 tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct ttccctgtta acgcccgatg
     5821 gcgcaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac
     5881 ggttcgatcc gtggctggtt gccgaagccg aagaagaagg tgtcgaatgc atccccggag
     5941 cgacggtgga tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg
     6001 atattctccg tgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctggctgagc
     6061 gtcacgggtt agtgactcgt cctgctggtg aagcgatggc gttggggatc aaagaagtgc
     6121 tgtcgctgga accatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag
     6181 cgttgctgtt cagcggcagg atctgtgatg acttacccgg cggcgcattt ctttatacta
     6241 atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg caaagtcgtg
     6301 ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc ccgcttatca
     6361 aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc ttgcacagta
     6421 tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg
     6481 tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgcg caggcggcgg
     6541 cacaaacgct gataagcgcc tgccagcacc gcgagccgca aaatctgttt gcgctttatc
     6601 atcacaacgt agagcgcagc ctgctatggg atgttctaca acgttatcag catgttccgg
     6661 cgcttttgca acgccctggc tggtatcggg cgtggcctgc gttaatgcag gatatttccc
     6721 gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta ttctggcgtc
     6781 atttacgtcg tcatggcctg tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc
     6841 tgtagcccat aatctctggc gcgttgctga tgcgccgcac attgttccgg ctgactccgt
     6901 tgagcgtcag atggtacaac ggttgattaa cgcctgtccg gcaggtcttt tttcgctcac
     6961 accggaaggt gacttacgcg ttgattatca cggctgcctg gagtgtggca cctgccgttt
     7021 gctgtgcgac gaatccacac tacaacagtg gcgctatccg ccttccggat tcggcatcac
     7081 ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg ctccgccaga
     7141 atccggtgat tgctgccgtt aaagataatg ccagcctgca actggcaatc gactctgaat
     7201 gccagtttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc gttaaaaaga
     7261 ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata
     7321 aagaagtcgt gattcagttt ttgaaactgg tgaccgaggc ggacggcatt atcagtacca
     7381 aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat cgcctgttta
     7441 ttgttgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt
     7501 gtattgagat cctgccaggc tgtatgccca aagtgctggg ctgggtgaca gagaaaatcc
     7561 gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta
     7621 acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta gcgaaaaaat
     7681 tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc cgcacgccat
     7741 atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta caagtcccca
     7801 ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc gtgaatgttt
     7861 gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc tgcctgtgaa
     7921 aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc
     7981 gccattaacg ctgctgcgcc agaagcgcca acaacaatac acacctcagg tgccagcaga
     8041 tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac tcgcgcccga
     8101 ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg gttatctgcc
     8161 tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc gcgagtaaca
     8221 tttttcagat cctctgtgct aaccagccag tcgggaagcg cacccgggac aatgttcagt
     8281 tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc ccgcctgacg cgccagggaa
     8341 aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc ccagtgggat
     8401 ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct tgcaccatgt
     8461 aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg aaagagaaca
     8521 acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg ttccgccact
     8581 accggctgag gttcaatctg ccagtgcgcc agggtacaac cgctgaaatc ctgcgccgcc
     8641 agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat attcatggct
     8701 gcatcctttg gcgtaaatag tcctgccata gcttttgagc tttttctgct accgtttgcc
     8761 catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga cactgcatcg
     8821 ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca gccattcgct
     8881 ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca atcaccgcag
     8941 gcaagcgaac ccggcaacaa cgcaacccat gttcagtacg ctgttcgagg gtgataaaca
     9001 gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc atttccgcca
     9061 gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta attatcagat
     9121 ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc
     9181 gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc atgagatagc
     9241 gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac
     9301 cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca ccgagtaaac
     9361 ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc cactcttttt
     9421 ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct
     9481 cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca tagccaggtc
     9541 acaacaaaac caaccaacaa cactgtcgcc aggagcaata aggtgacttg cattccccac
     9601 tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg actcatggcg
     9661 gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg
     9721 cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt
     9781 aatgaactgc cggaaggcaa acaggccatg actaccagcg ttgccgccag cagcaaaaaa
     9841 cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc caacagcgcg
     9901 cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata
     9961 gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca
    10021 aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa ggttttgatg
    10081 tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc aaaatagcga
    10141 tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggtgattct
    10201 ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagtagc
    10261 cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact tgccagcaca
    10321 tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg gcggcgcggg
    10381 gaaaattcag ccagcaaggt gtgacctact gaataatcgc ctcccagact aatgccaatc
    10441 aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgtaa aaacgaagca
    10501 agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg gtcggagatc
    10561 cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg ccgagccgcc gatcattccc
    10621 gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta accaatgacg
    10681 ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat gcggcagtga
    10741 aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca
    10801 ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccgtcatc
    10861 gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt attcataatg
    10921 ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct gccgtgttcc
    10981 agtttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat cgaaccgccg
    11041 aggcgaatgg tttcttcaca gatgatcttg ttgagcggat tgtggtactt gtcgatttcc
    11101 tcttccggct tacagtcaac gacgttgtaa tcgtagacga agtacatgtt ggtgccgttc
    11161 tgatagctat gagaggaatg accgcccagc atggtgatgt cgtcggcgtg cgggaactca
    11221 gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca gcagccggac
    11281 acttcagtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagccact
    11341 ttatccggtc cccagttcag gttgttaaac caggtttcga tcagcttgct gtccacgcgc
    11401 tggcattgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc cttcgcaatg
    11461 cgagggttac cttcagccat aaagatcagc acgcattttc cgtctgcaaa atgggtgaag
    11521 tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata cccttccacc
    11581 atgacttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata gccgtagaag
    11641 aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat atagcaaagt
    11701 gcaccttcgt taccgatgat gatgtggcga atgtccgggc cagccgcgcg gcgtggcacg
    11761 tttttaatgc gtgtgacggt gccatcagcc aatactgctt ccagaccaac gaccatatct
    11821 tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac caggccgccc
    11881 atctgagcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac
    11941 gcattttcca gcacctccag cggaacgcca cattgcgccg tcgcctgcat attctcaata
    12001 tcaatattaa tgatttgatt catggcggag ccgtcgagca ccaccgagtt ttccacaacg
    12061 gtttccagcc caccttcagt ggcggaagcg ccggtacgcg gcacaccgtt aattttgtgc
    12121 gcattcataa aattcagcac acgggagact tgttctgtgg aaccgagttt tacgaccgct
    12181 gccggaatcg gcaaagtata aataccatga atatccggaa atttacgaaa acggtcaata
    12241 ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac aatttccttt
    12301 agctggtcga caatcgctgc
//
LOCUS       DAZFWP020000141        12311 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFWP020000141 DAZFWP020000000
VERSION     DAZFWP020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.141"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..738)
                     /gene="mlaA"
                     /locus_tag="ACHK0Z_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             complement(<1..738)
                     /gene="mlaA"
                     /locus_tag="ACHK0Z_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU4503815.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLK"
     gene            1032..1964
                     /locus_tag="ACHK0Z_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             1032..1964
                     /locus_tag="ACHK0Z_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU4503816.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2040..2114
                     /locus_tag="ACHK0Z_RS19350"
                     /old_locus_tag="pgaptmp_003870"
     tRNA            2040..2114
                     /locus_tag="ACHK0Z_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2073..2075,aa:Arg,seq:cct)
     gene            complement(2556..3803)
                     /locus_tag="ACHK0Z_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             complement(2556..3803)
                     /locus_tag="ACHK0Z_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU4503817.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3875..4789)
                     /locus_tag="ACHK0Z_RS19360"
                     /old_locus_tag="pgaptmp_003872"
     CDS             complement(3875..4789)
                     /locus_tag="ACHK0Z_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU4503818.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5005..6438
                     /gene="cscA"
                     /locus_tag="ACHK0Z_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             5005..6438
                     /gene="cscA"
                     /locus_tag="ACHK0Z_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU4503819.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6446..7441)
                     /locus_tag="ACHK0Z_RS19370"
                     /old_locus_tag="pgaptmp_003874"
     CDS             complement(6446..7441)
                     /locus_tag="ACHK0Z_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4503820.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7684..7902
                     /locus_tag="ACHK0Z_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /pseudo
     CDS             7684..7902
                     /locus_tag="ACHK0Z_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7920..9244
                     /gene="dsdA"
                     /locus_tag="ACHK0Z_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /pseudo
     CDS             7920..9244
                     /gene="dsdA"
                     /locus_tag="ACHK0Z_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(9352..10890)
                     /gene="emrY"
                     /locus_tag="ACHK0Z_RS19385"
                     /old_locus_tag="pgaptmp_003877"
     CDS             complement(9352..10890)
                     /gene="emrY"
                     /locus_tag="ACHK0Z_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU4503821.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10890..12053)
                     /gene="emrK"
                     /locus_tag="ACHK0Z_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             complement(10890..12053)
                     /gene="emrK"
                     /locus_tag="ACHK0Z_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU4503822.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKETWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3463 a         2987 c         2803 g         3058 t
ORIGIN      
        1 ttttaaatca tcctgaatcg cttgcgcgtt cggattttcc tgcggtttga gttcgccgcc
       61 attagcgatg aaatcatgac gctggaagta cgcttcgcgc accataatat aaggatcgga
      121 cgactgacgc agcagaccat cggaatccaa caactgcgca cgagtttcta tcccttcaag
      181 cgtccattta cccacagaca tcggccaggt cagccaggaa agaaccgggt aaagaccatc
      241 cgccatatca ccaccgtcat cacgcagcgt gaagctaccg tagaacggta actggacgta
      301 aggcccataa cccacgccat aatgaccaag cgtactaccg aagcggtgag gttcagtccg
      361 ttgcagtttc gggttcgcca tccctgcaac atcaataaaa ccgcccatcc ccaaaatggt
      421 gttcaggaaa aagcgggtaa agtggaccat cccctgataa gggtcgccct gcaagaagta
      481 gttaaccatc accgcaggtt cttcaaggtt gccagtaaag ttgctcaaac cgttacgcgc
      541 cggttgcgga acataatcac gccaggcgac agcgaccggt cgaacaatat acgggtctaa
      601 tacattgaag ttgaagttgt acatggtgcg gttgaaccct tctaacgggt cagaacgccc
      661 ttgctgatct gtaccggaac tcgcacaccc caccagaagc gtagttccca gagcaagcgc
      721 cgacaggcga agcttcataa atgtctccct gtttttttat ggcttatgca gtttgccatc
      781 catgacggaa cgataccgta tccgcctgtt taggtgtggg cgattgtaac agcacgtcaa
      841 ctgatgtcca gacgccctga tttgctgatt tgatcatagc ctggtaatcg ccgccctgta
      901 ggctacttga ttctatagaa acagaaaaag gcaaacgcag ccttttctac attttcagag
      961 taactcccgc cagttgcgag caaaaaagcc gctacgcttt agctatacgt gctaattcaa
     1021 gagaagagac catggacaac gacaaaattg atcaacacag cgacgaaatt gaagttgaga
     1081 gcgaagaaaa agagcgcggc aaaaaaatag aaatagatga agaccgactc ccctcccggg
     1141 cgatggcaat tcatgagcat atccgccagg atggtgaaaa agagctggaa cgcgacgcaa
     1201 tggcgctact gtggtcagcc attgcggcgg gtctgtcgat gggcgcttcg ctactggcaa
     1261 aagggatatt tcatgtcgaa ctggaaggag tgccaggcag cttcttactg gagaatctcg
     1321 gttatacctt tggttttatt atcgtcatta tggcccgcca gcaattattt accgaaaaca
     1381 ccgtgactgc ggtactaccc gtcatgcaaa aaccgacaat gagcaacgtc ggcttactta
     1441 tgcgattatg gggcgtcgtg ctgctgggta atattctcgg gacaggtatt gctgcatggg
     1501 catttgaata tatgcctatc ttcaatgaag aaactcgcga tgcatttgtc aaaatcggca
     1561 tggatgtgat gaagaacacc cccagcgaga tgtttgccaa cgcgatcatt tccggctggc
     1621 tgatcgccac tatggtttgg atgtttcctg ctgcgggtgc ggcaaagatt gtggtgatta
     1681 tattgatgac ctggcttatt gcactgggtg acaccaccca catcgttgtc ggttctgttg
     1741 aaatcctcta tctggtgttt aacggcacgc tgcactggag cgatttcatc tggcccttcg
     1801 cactacctac tttagcgggg aacatctgcg gcggcacctt tatcttcgcg ttaatgagtc
     1861 atgcacagat tcgtaacgac atgagcaaca agcgtaaagc agaagcacgc caaaaagcag
     1921 aacgtgcgga aaacattaag aaaaatgata aaaacccagc ataaatggcg agggtttaag
     1981 caatcgagcg gcagcgtact taccccgcag tccattagcg ggtatactca tgccgcattg
     2041 tcctcttagt taaatggata taacgagccc ctcctaaggg ctaattgcag gttcgattcc
     2101 tgcaggggac accagatacc cttcaaacga aatctacctt caccccgtaa aagatgggtt
     2161 tggcagcaca cttgccttat atctactcat ttttactgca acaggttgaa atctcagcac
     2221 tgtcagaaag cgctgatgac taaacagccc tgggccgggc gatgtaacca tcacacagaa
     2281 tcctgatagc gaaatatggc gtgactcgat acttcactcc gcaatgcatt ccttgatgaa
     2341 ttcgcaggac cgtgatacac gggacaggtc actgaatgac gacaatgtcc tggaaatcag
     2401 cgaaccgcgc atctgaagta catttgagcg actgtaccag aacatgaatg aggcgtttgg
     2461 attaggcgat tattagcagg gctaagcatt ttactattat tattttccgg ttgagggata
     2521 tagagctatc gacaacaacc ggaaaaagtt tacgtctata ttgctgaagg tacaggcgtt
     2581 tccataacta tttgctcgcg ttttttactc aagaagaaaa tgccaaatag caacatcagg
     2641 cagacaatac ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc aaagagtatc
     2701 ccagtcggcg ttgaaagcag cacaatccca agcgaactgg caatttgaaa accaatcaga
     2761 aagatcgtcg acgacaggcg cttatcaaag tttgccacgc tgtatttgaa gacggatatg
     2821 acacaaagtg gaacctcaat ggcatgtaac aacttcacta atgaaataat ccaggggtta
     2881 acgaacagcg cgcaggaaag gatacgcaac gccataatca caactccgat aagtaatgca
     2941 ttttttggcc ctacccgatt cacaaagaaa ggaataatcg ccatgcacag cgcttcgagt
     3001 accacctgga atgagttgag ataaccatac aggcgcgttc ctacatcgtg tgattcgaat
     3061 aaacctgcat aaaagacagg aaaaagttgt tgatcaaaaa tgttatagaa agaccacgtc
     3121 cccacaataa atatgacgaa aacccagaag tttcgatcct tgaaaactgc gataaaatcc
     3181 tcttttttta cccctcccgc atctgccgct acgcactggt gatccttatc tttaaaacgc
     3241 atgttgatca tcataaatac agcgccaaat agcgagacca accagaagtt gatatgggga
     3301 ctgatactaa aaaatatgcc ggcaaagaac gcgccaatag catagccaaa agatccccag
     3361 gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt gaagctatca
     3421 agcaaaccgc atcccgccag ataccccaag ccaaaaaata gcgcccccag aattagacct
     3481 acagaaaaat tgctttgcag taacggttca taaacgtaaa tcataaacgg tccggtcaag
     3541 accaggatga aactcataca ccagatgagc ggtttcttca gaccgagttt atcctgaacg
     3601 atgccgtaga acatcataaa tagaatgctg gtaaactggt tgaccgaata aagtgtacct
     3661 aattccgtcc ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa cgaccaccac
     3721 agcgaccagg aaataaaaaa gagaaatgag taactggatg caaaacgata gtacgcattt
     3781 ctgaatggaa tattcagtgc cataattacc tgcctgtcgt taaaaaattc acgtcctatt
     3841 tagagataag agcgacttcg ccgtttactt ctcactattc cagttcttgt cgacatggca
     3901 gcgctgtcat tgcccctttc gccgttactg caagcgctcc gcaacgttga gcgagatcga
     3961 taattcgtcg catttctctc tcatctgtag ataatcccgt agaggacaga cctgtgagta
     4021 acccggcaac gaacgcatct cccgcccccg tgctatcgac acaattcaca gacattccag
     4081 caaaatggtg aacttgtcct cgataacaga ccaccacacc ttctgcacct ttagtcacca
     4141 acagcatggc gatctcatac tcttttgcca gggcgcatat atcccgatcg ttctgtgttt
     4201 ttccactgat aagtcgccat tcttcttccg agagcttgac gacatccgcc agttgtagcg
     4261 cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca tagatcttca cgaatattag
     4321 gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt catcgcagta aatgcgctgg
     4381 tacgcgaagg ctcggcagac aacgcaattg aacagagatg taaccattcg ccatgtcgcc
     4441 agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc ataaacgtaa
     4501 atgaacgttc cccttgatcg ttcagatcga caagcaccgt ggatgtccgg tgccattcat
     4561 cttgcttcag atacgtgata tcgactccct cagttagcag cgttctttgc attaacgcac
     4621 caaaaggatc atcccccacc cgacctataa acccacttgt tccgcctaat ctggcgattc
     4681 ccaccgcaac gttagctggc gcgccgccag gacaaggcag taggcgcccg tctgattctg
     4741 gcaagagatc tacgaccgca tcccctaaaa cccatacttt ggctgacatt tttttccctt
     4801 aaattcatct gagttacgca tagtgataaa cctctttttc gcaaaatcgt catggattta
     4861 ctaaaacatg catattcgat cacaaaacgt catagttaac gttaacattt gtgatattca
     4921 tcgcatttat gaaagtaagg gactttattt ttataaaagt taacgttaac aattcaccaa
     4981 atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgcc
     5041 ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcct
     5101 cctgccgggt ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg
     5161 ttttatcaac atcatccgat gagcgaacac tgggggccaa tgcactgggg acatgccacc
     5221 agcgacgata tgatccactg gcagcatgag cctattgcgc tagcgccagg agacgataat
     5281 gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc
     5341 tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa
     5401 tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca
     5461 ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg
     5521 atggtagtcg gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt
     5581 tcattgcgtg aatggacctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat
     5641 atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg
     5701 cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc
     5761 ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga acttgataac
     5821 gggcatgact tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc
     5881 ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc
     5941 atgacgctgg cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggttcac
     6001 gaagctgagt cgttacgcca gcagcatcaa tctgtctctc cccgcacaat cagcaataaa
     6061 tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac
     6121 agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac
     6181 caatctgagc gacttgtttt gtggcggtat tacccacacg agaatttaga cggctaccgt
     6241 agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc
     6301 gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca
     6361 gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca
     6421 ctctggctac tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga
     6481 tccgcgtcac tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga
     6541 taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa
     6601 ccagattatc aaagcccatt acggcgacat cctgcgggat acgtaccccc ttcgccagga
     6661 gaacctgata agcgacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg
     6721 gtttgcccgg tttgaagtgg gcattgagta aacttgcgag atcggtgtag tgatcatcac
     6781 ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaggcct
     6841 gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata
     6901 acggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt gcagtgtaat
     6961 catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat
     7021 tttcaccata cagagactca ggcagcgtga tatgtcgcag ccccattgta gtatagataa
     7081 tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa
     7141 aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca
     7201 gaatatcaac agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg
     7261 gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct
     7321 ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca
     7381 cccgggagac tgtcatcatc gacactcccg ccaggcgtgc gacatccttt aatgaagcca
     7441 tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat
     7501 ccaacgatat tctcaacgcc ctatcaccca acacggcaat acaataaaaa ataacaataa
     7561 ttgccggata attgtactca attccgcctc tgttctcgcc aacgagtaac catataggca
     7621 agtaagtaaa acatgccaac tggcatcgcc aacctggaga agaaaatata aatatggatc
     7681 attgtgctgc cgctgtatcc cgacatcagc ccggaaatta ttgcgattgc tatcggttca
     7741 ggtgcaattg gctgcacgat cgttacggac tcgcttttct ggctagtgaa gcaatattgc
     7801 ggcgctacgc tcaatgaaac atttaaatac tatacgacag cgacatttat cgcttcagtc
     7861 gtcgctctgg cgggcacatt cctgctgtca tttatcatct aagcgcaaag agacgtacta
     7921 tggaaaacgc taaaatgaac tcgctcatcg cccagtatcc gttggtaaag gatctggttg
     7981 ctcttaaaga aaccacctgg tttaatcctg gcacgacctc attggctgaa ggtttacctt
     8041 atgttggcct gaccgaacag gatgttcagg acgcccatgc gcgcttatcc cgttttgcac
     8101 cctatctggc aaaagcattt cctgaaactg ctgccactgg ggggattatt gaatcagaac
     8161 tggttgccat tccagctatg caaaaacggc tggaaaaaga atatcagcaa ccgatcagcg
     8221 ggcaactgtt actgaaaaaa gatagccatt tgcccatttc cggctccata aaagcacgcg
     8281 gcgggattta tgaagtcctg gcacacgcag aaaaactggc tctggaagcg gggttgctga
     8341 cgcttgatga tgactacagc aaactgcttt ctccggagtt taaacagttc tttagccaat
     8401 acagcattgc tgtgggctca accggaaatc tggggttatc aatcggcatt atgagcgccc
     8461 gcattggctt taaggtgaca gttcatatgt ctgctgatgc ccgggcatgg aaaaaagcga
     8521 aactgcgcag ccatggcgtt acggtcgtgg aatatgagca agattatggt gttgccgtcg
     8581 aggaaggacg taaagcagcg cagtctgacc cgaactgttt ctttattgat gacgaaaatt
     8641 cccgcacgtt gttccttggg tattccgtcg ctggccagcg tcttaaagcg caatttgccc
     8701 agcaaggccg tatcgtcgat gctgataacc ctctgtttgt ctatctgccg tgtggtgttg
     8761 gcggtggtcc tggtggcgtc gcattcgggc ttaaactggc gtttggcgat catgttcact
     8821 gcttttttgc cgaaccaacg cactcccctt gtatgttgtt aggcgtccat acaggattac
     8881 acgatcagat ttctgttcag gatattggta tcgacaacct taccgcagcg gatggccttg
     8941 cagttggtcg cgcatcaggc tttgtcgggc gggcaatgga gcgtctgctg gatggcttct
     9001 atacccttag cgatcaaacc atgtatgaca tgcttggctg gcgcaggaag aaggtattcg
     9061 tcttgaacct tcagcactgg cgggtatggc cggacctcag cgcgtgtgtg catcagtaag
     9121 ttaccaacag atgcacggtt tcagcgcaga acaattgcgt aatgccactc atctggtgtg
     9181 ggcgacggga ggtggaatgg tgccggaaga agagatgaat caatatctgg caaaaggccg
     9241 ttaataacgt ttcaacgcag catcgcaatc ctttccctgg gtgagcgatg ctgccgatgg
     9301 cgcagactta agatccccgg tcttacccgc tataaccccc tccttttatc atcacccaac
     9361 gcctttcgct gtaaacggcg gtttcgcaaa ccaaaccaaa accgtaagta agataaaagc
     9421 aatagccgcc atacggaaaa tctcatttgc agaaatagaa agtgactgct gggttatttc
     9481 attattaatt tcattaagaa ctcctgaaag cgaaccatag tatttatcca taatttgtga
     9541 cgatgaatta aacacggggt taaattgatc gatggttgct gtcaactgac tatggtgtaa
     9601 cgattcgcgt cgtccccaca gcgtcattgt caacgacgta ccaactgatc ctgacaaggt
     9661 acgaaaaaaa ttactcatac tcgaggcatt ggcaaattta ttatctggca agcctgaaaa
     9721 cgaaatcgtt gttaagggta aaaagaaaca ggcaacggcg aatccctgaa aaaactgcgg
     9781 caaaatgatg cctgtaaaat caatcgttgg cataaatgtc acagaacgcc agtaatagca
     9841 aaccgcatac atcaaaaaac taaatgtcac taacaaccgc atgtctattt tgttgccata
     9901 acgtcctatc aaaggtgaaa ttaatagtgg catgatgccg atgggcgcat aagcaagtcc
     9961 ggcccatatc gcattatacc ccatcgtttc ctggagtaac tgcggcataa ggacgatcgc
    10021 tccagagtaa aataaatacg cgcatgtgat actcacaata ccaatggtga agttacggga
    10081 cttaaacaaa ctgagatcaa gaatcgggtt ctctgaggtc gactcccaaa tgactaaaga
    10141 gatcagagaa ataactgata ctactgttaa tattattatt gtactcgagt tgaaccaatc
    10201 cagatcgcgc cctttatcaa gcataatttg caagccacca acaccgagca ctaacagggt
    10261 cagtcctggt agattcattt tgaccggtga agtttcagtt tctcttcctt taagtaaggt
    10321 taagcataat gtcaggacga taatccccat agggacattg attaaaaata tccaacccca
    10381 gctaaagtta tcgcaaatat aaccgcccaa tatcggccca catatcggag cgataatcac
    10441 ggtcattgac cataatgcca gagcaaatgt tcttttttct ggtggataat tccttaataa
    10501 taaactctgt gacagtggaa ttaacggccc cgccattaat ccctgaacga ctctaaaaaa
    10561 tatcagcaca tcaagattgg tcgataaact acacattaag gaagacagcg aaaaaaaagt
    10621 gactgaaagt aaaaataacc ttaattcgcc tattctttgt gccagcctgc cggtaacagg
    10681 gatcgcaatg gcatttgcta cgccaaacga ggtgataacc caggtgcctt cgtctgttga
    10741 tgctcccaga aagccagata ttgtcggtat tgcgacgtta gaaatagtgg aatccaacat
    10801 ttgcataaac gtcgctaatg acaatgcaat agtaacgcac cataacgtcc caccggttaa
    10861 tggtgccgga gtcgatttag tgattgccat taaagttgtc cattatgcga aataatgttg
    10921 ctaatttctt tttctatcgg actggtatcg ataactaaag ccttactggt ataagccggc
    10981 atggacgtca cggttgaagc cagatcaggc atctcagcaa tgtcttcgtt cttcgtatca
    11041 atagttgcag tcatcgataa accaatgcgt aaggggtgtt ccatgagttc ttttggatca
    11101 agagaaactt caaccggtac acgctgaacg attttgatcc agttccctgt cgcattttgt
    11161 gcaggtaata aggagaacgc attgccggtt cccatattga tccctgtcac ccgaccatga
    11221 aacacaacat tttcaccata aagatcgctg ataatattga ctgattgacc aatccgtaca
    11281 tccgtgagtt gtgtttcttt aaagttggca ttaacccaca tttgacgtgc cggtacgaca
    11341 gccattaacg attgtccggg actcactgtt tcgccgacct gaacactccg ctgggcaata
    11401 tagccagtaa ccggactcct tatatccgta cgtttaagcg ccaaccaggt ttctttagtt
    11461 gcatccgccg cttccacgac ttgtggctga cggtttaatg gtgtgttcat tactaaagct
    11521 ttattcgctt tataagcctg gatagcggca ttcagtgccg ctttgctact tattaacgta
    11581 tctttggtat gctccagcgt ttcttttgaa ataaccccct gcttcgctaa cggtactcga
    11641 cggttataat cttctaaaga ttgttgatac tgaatacgtg ctgaagcgac ttcggcactg
    11701 tattgtttat cctgtaagta cagtttattc gtttgccgaa caatatttgc cagattattt
    11761 ttagctttat tgagtgcgat agtggcatca gttttatcca gtgaaactaa aatgtcacct
    11821 tgtcgaacgt agttcgtatc tttatgatta acgacagtga cactacctga gacttgtgca
    11881 gaaattggat ctgcattccc cgtgacatag gcgtcatctg tactaatcat gtcttctaat
    11941 tccattgacc aataggcata ggcacctgaa aacgcaataa ataaaactac cgccaataaa
    12001 gaaaagtatt ttcttctgtt agaatgtttt ttatttgaat taatctgttc cactattatc
    12061 tctcatttct catagatgaa atttatgaat tgaatgaaaa gagcattcat tcttcatatc
    12121 aaacacagtg catgtgttgc acattatccc aggaacgttg gaggcctaat ttaaatgagg
    12181 tgcaaaaaaa acaacagagg actaaaccgt ggcttttgca atacaattct tacgcctgta
    12241 ggattagtga gaagacttat agtgctaact tgaaactata aatcatcggt ataatccctg
    12301 attttattgt t
//
LOCUS       DAZFWP020000142        12275 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFWP020000142 DAZFWP020000000
VERSION     DAZFWP020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.142"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(34..315)
                     /gene="yehE"
                     /locus_tag="ACHK0Z_RS19395"
                     /old_locus_tag="pgaptmp_003879"
     CDS             complement(34..315)
                     /gene="yehE"
                     /locus_tag="ACHK0Z_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGU4503823.1"
                     /translation="MNKYWLSGIIFLAYGLAFPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(578..1687)
                     /gene="apbC"
                     /locus_tag="ACHK0Z_RS19400"
                     /old_locus_tag="pgaptmp_003880"
     CDS             complement(578..1687)
                     /gene="apbC"
                     /locus_tag="ACHK0Z_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU4503824.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIVSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPGLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1819..3852
                     /gene="metG"
                     /locus_tag="ACHK0Z_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             1819..3852
                     /gene="metG"
                     /locus_tag="ACHK0Z_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU4503825.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3993..7787
                     /locus_tag="ACHK0Z_RS19410"
                     /old_locus_tag="pgaptmp_003882"
     CDS             3993..7787
                     /locus_tag="ACHK0Z_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HGU4503826.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7797..11429
                     /gene="yehI"
                     /locus_tag="ACHK0Z_RS19415"
                     /old_locus_tag="pgaptmp_003883"
     CDS             7797..11429
                     /gene="yehI"
                     /locus_tag="ACHK0Z_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU4503827.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARLGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11490..11810
                     /gene="yehK"
                     /locus_tag="ACHK0Z_RS19420"
                     /old_locus_tag="pgaptmp_003884"
     CDS             11490..11810
                     /gene="yehK"
                     /locus_tag="ACHK0Z_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU4503828.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
BASE COUNT         3205 a         3133 c         3008 g         2929 t
ORIGIN      
        1 ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg
       61 acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga cgggcgaaga
      121 aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt gaggttgacc
      181 attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg ctaatgtggc
      241 agtctctgaa gagaatgcgg gaaaagccaa tccataggca agaaaaataa ttcctgacag
      301 ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt
      361 gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt
      421 gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt
      481 attaaataag tgttcattaa tgcggcaaaa actaatacac cgcatcaatg taacatctct
      541 gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct
      601 cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc
      661 ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga
      721 gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgtg tggtattttt
      781 cagccagttt ctcagcacca ccggtaccga aaatcggttc atggtgacca cagttactgc
      841 aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact ttctcgaaca
      901 tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg
      961 caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca
     1021 tatcaagcac cagatagtcg agacccggcc acaaggtttc ctgcaacatc tgcattaacg
     1081 ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc agataaccaa
     1141 ttgaattggt tgccaggcca tgagacacaa tcggtgccat atgggtaccg tccggcgaag
     1201 ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt
     1261 ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg
     1321 aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acaccattaa
     1381 tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga
     1441 tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa
     1501 acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg
     1561 cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat
     1621 tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt
     1681 cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa
     1741 tatagagaag tacttactta acattttccc atttggtact atctaacccc ttttcactat
     1801 taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg
     1861 ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc
     1921 tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc
     1981 cacggtacac cgatcatgct gaaagctcag cagctcggta tcaccccgga gcagatgatt
     2041 ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac
     2101 tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg
     2161 aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc
     2221 atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa
     2281 tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt gatcgagccg
     2341 aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat
     2401 ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag
     2461 gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga tatctcccgc
     2521 gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg
     2581 ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac
     2641 agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc
     2701 ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga aggcagcaac
     2761 ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg
     2821 tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac
     2881 agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac
     2941 ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt taacctggcc
     3001 tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaattggct
     3061 gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa
     3121 agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc
     3181 tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgccgacctg
     3241 caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta cctgaagccg
     3301 gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact gacctgggat
     3361 ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct gtataaccgc
     3421 atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct
     3481 gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac
     3541 gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt tgttgaaggt
     3601 tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc
     3661 ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac cattatggtg
     3721 gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt aatggctgct
     3781 ggtcctggcg ggaaagatat cttcctgtta agcccggatg ctggtgctaa accaggccat
     3841 caggtgaaat aatccccctt taaggcgctg catcgacagc gccttttctt tataaattcc
     3901 taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatctg
     3961 cattcaactg gataaaatta cagggatgca gaatgagaca ctttatctat caggacgaaa
     4021 aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc agttggggaa
     4081 aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg gcagtggcaa
     4141 aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag caagcgaagg
     4201 ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg gacttatcca
     4261 ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac aaaaatacag
     4321 atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac
     4381 acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga
     4441 cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca gccacatttg
     4501 acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg
     4561 acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg gcacttgaaa
     4621 tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg
     4681 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt
     4741 atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac ttggagcaat
     4801 attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc
     4861 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatgg cggcaaccat
     4921 taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     4981 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     5041 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
     5101 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     5161 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     5221 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cggatgacga
     5281 aagcctgcgc tgcgttccca catgcagcaa tggcggcact gacggaactt cttgggcaaa
     5341 aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa ccagcactgg
     5401 cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc
     5461 aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc gtagtagtct
     5521 cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5581 taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5641 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5701 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5761 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5821 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag
     5881 taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac gccatgttac
     5941 attttggact caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag
     6001 ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct
     6061 tcaacaaact gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac
     6121 atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca caggataacg
     6181 cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg caagaaatcg
     6241 cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc
     6301 ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct
     6361 ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc ctcctccacc
     6421 tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta ttgcaggtga
     6481 aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt accgcctggc
     6541 agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc gttctcggta
     6601 acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt gaatcccagc
     6661 ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctta
     6721 tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag
     6781 aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt gaagatcggt
     6841 tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt ggcccacggc
     6901 agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc
     6961 gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga aacgcgggcg aacgatgcgg
     7021 ttaaccgcta caaattgctg aaaaaagatg cgcgtaccat cgccgcccag caggtagcaa
     7081 ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgct ggaaaacttc cagctcttcc
     7141 tggttgagca tccgctggtt cgtcacttaa cccgccgtct gatttggggc gtttatagcg
     7201 ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac agcaccgctg
     7261 acgatgatct tttcaccctg ccggaaggcg atatctctat cggcactcct cacgttctgg
     7321 aaatatcacc aacggatgct gccgcctttg gtcagctttt tgccgactac gaactgctac
     7381 caccgttccg ccagctcgac cgtaacagct acgccctgac agaagccgag cgcaatgcca
     7441 gtgaactgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg ggactggcga
     7501 ataaaggttg ggtgcgtggt gaaccgcagg atggtggctg gatcggctgg atgatcaaac
     7561 ctctgggccg ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt ggtatgtcgc
     7621 cagccgaact tagcgccgag caactcttaa gcaagctgtg gctatgggaa ggcaaagcag
     7681 aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca
     7741 tcaccgccag cgagctgatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     7801 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     7861 aaacaccact cagccatcgc aattggccgg gcgaaccagt gcctgttatc actgaaagca
     7921 tcattcagac actgggtgat gaattgctac aaaaagctga gaagaaaaaa aacattgtct
     7981 ggcgttatga gaatttttca ctggagtggc agtccgccat tacgcaggcc atcaacttga
     8041 tcggcgaaca caaaccctca atcccggccc agacaatggc ggcgctagtc tgtatcgcgc
     8101 aaaatgacag ccaacagttg ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga
     8161 ctgaggtggt gattgcacgc cagtttatta cacgatgtta tgagagtgat cctctggtag
     8221 tcacattgca gtatcagaac gaggactatg gctatggtta tcgctcagaa acctataacg
     8281 aattcgatct ccgactgcgt aagcatctct ctctggcaga ggaaagctgc tggcagcgtt
     8341 gcgccgacaa actcattgcc acactgccag gaataaccaa agttcgccgc ccttttattg
     8401 cgctgatcct ccccgaaaaa ccagaaatcg ccaatgagtt ggtaagcctt gaatgcccgc
     8461 ggactcattt tcattctaag gagtggttaa aagttgttgc taatgacccc acagcggtga
     8521 gaaaactcga acgctactgg agccaggata tatttagcga tcgagaagcc agctacatgt
     8581 cgcatgaaaa ccacttcggc tacgcggcct gcgccgccct tttgcgcgaa caaggactgg
     8641 cagccgttcc gcgcctcgcg atatatgccc ataaagaaga ttgcggcagt ctgctggtac
     8701 aaattaacca tccgcaagtc atccgcacct tgctgttagt tgctgataaa aacaaaccca
     8761 gcctgcaacg tgtggctaaa tacagtaaaa acttccccca tgcgacactc gccgcactgg
     8821 cagaactgct ggcgttaaaa gaaccaccag cccgcctagg ttatccaatc atcgaagaca
     8881 aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc
     8941 tgatagcatc gcagccacaa ctagcagaaa gggtgatgcc gtggttaagt actcaagcca
     9001 gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat agcaccgata
     9061 acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag
     9121 ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg caaccaggcg
     9181 aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgg
     9241 aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg
     9301 tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca
     9361 ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc
     9421 cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca
     9481 tatgggaaaa tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt
     9541 tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc attgagaaaa
     9601 agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag
     9661 gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg
     9721 gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg
     9781 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc
     9841 ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt
     9901 acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg
     9961 tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca
    10021 ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta
    10081 ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
    10141 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
    10201 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
    10261 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga
    10321 cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga
    10381 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    10441 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa
    10501 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    10561 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    10621 acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc
    10681 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    10741 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
    10801 cccgacgctg gtcgctggca gattttcata ctctgtttgt taatcatccc tttacccgcc
    10861 tggttaccca gcgattaata tggggcgtgt atccggcaaa tgaaccgcgt cgtttactca
    10921 acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaggatgag ccaattgacc
    10981 tgcctgcgga tgctctgatt ggcattgccc acccgttaga aatgacagca gaaatgcgca
    11041 gtgaatttgc ccagcttttt gccgattacg aaattatacc gccacttcgc cagttgacac
    11101 ggcgcacggt gctgctcacg cctgacgagt cagccagtaa tagtttgaat cgctgggaag
    11161 gtaaatctgc taccgttggg caacttatgg gaatgcgata caaaggctgg gagtcaggct
    11221 atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg
    11281 gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgt
    11341 acagtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt
    11401 taagcgcacc tgacgtcatt ttccattaaa acacagcggg cagtgatgca actgcccgtt
    11461 atcaacacga cttaccagcg gggaaagcga tgatcgtgca aaaagagctg gttgctattt
    11521 acgattatga ggtccctgta cctgaagatc cgttttcctt cagacttgag atccataaat
    11581 gctctgaatt atttacaggt tccgtctatc gactggagcg attccggcta cgtccaacat
    11641 ttcatcaacg tgatcgagaa gatgctgacc cgctaataaa tgatgcgttg atttatataa
    11701 gagatgagtg tattgatgag cggaaattac gaggtgaatc acctgaaact gtaatagcaa
    11761 tttttaatcg tgaactacag aatatattca accaactaat agaatattag tttattaaag
    11821 ggggaagcgg ttttccccct tagttgcgtt tcaatttgcc cattacttca acaaatcaat
    11881 caaccgtaac agagacacta atagctccag tacggcaatg agcacggtta acttttgtgt
    11941 cagcgtgtcc atccgctctc cttattaagg agcgcaacac cagcacctta cccttacact
    12001 gcctgtcgat actggtttac gtttgttgag tcgccatgtt gcactccgga tcagagcact
    12061 acctgcggca ccacccgtcc ggtagccagg acttgaaatg ggttcgatcc ggcgcaactt
    12121 cccaacagtc aggattataa atctcattat cttaattgca acactgtcca gaggtggtta
    12181 taattcgttc gttgagatgc catgttgcac tatcagtaac ttaacatcaa agtagtaaat
    12241 aaaagaacct gcctcaccag caggtttttt tattt
//
LOCUS       DAZFWP020000143        12145 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFWP020000143 DAZFWP020000000
VERSION     DAZFWP020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.143"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            277..786
                     /gene="tssB"
                     /locus_tag="ACHK0Z_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             277..786
                     /gene="tssB"
                     /locus_tag="ACHK0Z_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4503829.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGSGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKL
                     RRRRTFNPK"
     gene            835..2373
                     /gene="tssC"
                     /locus_tag="ACHK0Z_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             835..2373
                     /gene="tssC"
                     /locus_tag="ACHK0Z_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4503830.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGENVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFSNLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWVRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2391..3728
                     /gene="tssK"
                     /locus_tag="ACHK0Z_RS19435"
                     /old_locus_tag="pgaptmp_003887"
     CDS             2391..3728
                     /gene="tssK"
                     /locus_tag="ACHK0Z_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4503831.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALMSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLHALTNDSAVVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDEGYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFPLLSELLEASLPSRVIALDLGSLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3725..4390
                     /gene="tssL"
                     /locus_tag="ACHK0Z_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             3725..4390
                     /gene="tssL"
                     /locus_tag="ACHK0Z_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HGU4503832.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     GVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKDPLQAHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4403..6055
                     /locus_tag="ACHK0Z_RS19445"
                     /old_locus_tag="pgaptmp_003889"
     CDS             4403..6055
                     /locus_tag="ACHK0Z_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4503833.1"
                     /translation="MRNTLKQAVVLWGMVLLLVLWSVFISPSGVLIWAGAAAIVLTVA
                     ALLIYRRRQAWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVSDEEQLVAQAERLLTLRPAWASQLAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEIHVATSAETASPAQWLTQTSTQE
                     RSQQLCYLLKAESLMQWLNLNMLAALNGPETKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDATLPFPDALLRRLPRQSGFTPLRRACVTMLGITTVAGIAALC
                     LSATENRQLLRHIGDDLHQFYAVPAEEFITKARRLSVLKDDAIMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            6113..6604
                     /locus_tag="ACHK0Z_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     CDS             6113..6604
                     /locus_tag="ACHK0Z_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4503834.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFIFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKEPSKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6795..9429
                     /gene="tssH"
                     /locus_tag="ACHK0Z_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             6795..9429
                     /gene="tssH"
                     /locus_tag="ACHK0Z_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            9441..11948
                     /locus_tag="ACHK0Z_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             9441..11948
                     /locus_tag="ACHK0Z_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4503835.1"
                     /translation="MGIQNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLARSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMAP
                     AMAILDGAQSQMQSLSTDAQTTNADPADLSSQIALLQQSVKDLTQAVILLSAPKGVAI
                     ASGEHLQLAASKNVIANAGNNADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKM
                     SGITDENGNTELISSDKEEVVNISVFEPDEFLDDDIN"
BASE COUNT         2882 a         3310 c         3480 g         2473 t
ORIGIN      
        1 cccaatttct taaaaacgca aatttgtgaa gtttatcttg ttattatttt aagtttcatg
       61 ccaaattttc tcaccagata aggctgccct ctcccgaaaa ataatcaaga ggctaaataa
      121 tatctaaaat gatacaaccg tatctattcc ccagaaaaat acattattca tttttatatt
      181 ttcctaatca ttgcttttta tttaaatcac ccgataatcc cgtgaatata attatgtcaa
      241 taaccatcag aaaaagtgga tgatgaggaa aaggatatgg ccgacagttt ccagaatgaa
      301 gttcccgccg ctcgtgtaaa tatcaagctt gacctgcata caggcaatgc taaaaagaaa
      361 gttgaactcc ccctcaagct tcttgctgtt ggcgattaca gtaacggaaa agagcaacgt
      421 ccgctgtccg aacgggacaa gattgatatc aataaaaaca acttcaacag tgtcatggct
      481 gagttttcgc ctgcggttaa tttaacagta gaagacacac taagcggaag cggtaatgag
      541 caaaatatcg cgcttgaatt taaaagcctg aaagattttg aacccgaaca ggtagcaaaa
      601 aatatcccac agcttcgtgt attactcgct atgcgcaatt tattacgtga cctgaaatcc
      661 aatctgctgg ataacgcaac gtttcgccgt gaactggaaa atattctgaa agacccaacg
      721 ctcagttcag aactgcgtga tgaactggcg aaattgcgcc gcaggagaac gtttaacccg
      781 aagtgatatt caggttaaac cggagaagaa aggatcactg aaaaggaaat gctgatgtct
      841 gtacaacaag aacatgccac ctctgaaact gcaacactga ccaccactga gtccggcggc
      901 gtttatcagt ccctgtttga taaaattaat ttaaccccgg tgtcttccat tcaggaaata
      961 gatttatggc aaaacagcga aacactggcg gatgcctcac ccgatgagcg cgtgacagcg
     1021 gcgattcacg ttctgctttc ctgtctggcg aaatcaggcg agaacgtggt caagctcgac
     1081 aagagcctgc tggattttca tatcgacgat ttggatcaga aaatcagtaa gcagcttgat
     1141 gcggtcatgc accatcctga attccagaaa gtcgagtcgc tgtggcgcgg cacatggttc
     1201 gtcgtacagc gcactgattt tcgcaaaaat gtcagaattg aattgctgga tatcagcaaa
     1261 gagcatctgc gtcaggattt cgacgactcc ccggaaatca ttcagagtgg tttatatcgt
     1321 catacatata ttcaggagta cgatacgccg ggtggcgaac ctgttgcctc attaatttcc
     1381 agctatgaat ttgataacag cccgcaggat attgccctgc tgcgcaatat ttccagagtg
     1441 tctgccgctt cccatatgcc ttttatcggt tctgtcgggc cgaaattctt ccttaaaaat
     1501 tcgatggaag aagtcgccgc gattaaagat atcggcaact actttgaccg cgcagaatat
     1561 attaaatgga aatcgtttcg tgataccgat gacagccgct atgtgggatt agtgatgccg
     1621 cgagtgctgg gccgtctgcc ctatgggccg gacacggtgc cggtacgcag ctttaactat
     1681 gtggaagaag tcaaaggccc ggatcacgaa aagtatctct ggacaaacgc ctcgttcgcc
     1741 tttgccgcca atatggtgaa gagctttgtg aataatggct ggtgcgtgca gatccgcggt
     1801 ccacaagcag gtggcgcagt ggccgatctg cccatccatc tttacgatct cggcaccggc
     1861 aatcaggtca aaattccgtc cgaagtgatg atcccggaaa cccgcgaatt tgaattttcc
     1921 aaccttggct ttattccgct ctcttattat aagaatcgcg attacgcctg cttcttctcg
     1981 gcaaactctg cccagaaacc ggcgttgtat gataccgctg acgccaccgc caacagccgt
     2041 attaacgccc gtctgcccta tatcttcctg ctgtcccgca ttgcgcatta cctgaaaatc
     2101 atccagcgcg agaatatcgg taccaccaaa gaccgccgcg tactggaact ggaactgaac
     2161 acctgggtcc gcacactggt gacggagatg accgatccgg gtgatgaact gcaggcgtct
     2221 cacccgctgc gcgacggtaa ggttatcgtg gaagatattg aggacaatcc gggcttcttc
     2281 cgcgtcagac tctttgccgt gccgcatttc cagattgaag ggatggatat caacctgtca
     2341 ctggtttccc agatgccgaa agcgaaagcc tgaccgggaa gtggtgtgat atgaaaattc
     2401 atcgaccact gtgggctgag gggacattcc tgtcctctca gcagtttcag cagcaggccc
     2461 gctgggaagc gttcagcaat gacagcattg cgcaactttg tattcgtcat ccgtggggga
     2521 tcgcgaatgt gctctttgac cgcgatgccc tgatgtccgg aaagctgaaa acgcaggcag
     2581 tacgcctgcg ttttgctgat ggcacgctga tcgattctga cgtgagcgat gttctgcctc
     2641 tcgcctgcga tctgcacgcg ctgacgaacg acagcgcggt tgttctgctg gcgctgccgc
     2701 ttgcccatgg taacggcggt aatctcggtc agggtgagca gactgaaaga ccgctacgct
     2761 atcgtcagga atggcaaaag gtacaggata tctacggcag tgacagtgaa gatatggccg
     2821 ttgagcgtca tgcgctgtcc ctgcgctttg ctcatgataa caatcaggat tacatcacct
     2881 gcccactggc gcgtctggtg cgggatgttc agggcaactg gacacaggat gagggctata
     2941 ttccgccgtt actggcgttt aatgctcatg acgggttggt acagcgtctg gacacgctgc
     3001 tgttgcagct acgggccaaa tgtcagcgcc tgatggcgat gcgtcgggaa agcaaccagc
     3061 gtatggcgga cttcgccgtc gccgatgtct ccctgttctg gttgcttaac gcgctgaact
     3121 ccgccgagcc ggtgctgagt gatttcctgc gctacccggc ggttcatccc gaactggtct
     3181 ggcgggagct ggcgaggctt gccggagcgc tgctgacgtt ttcccttgaa cataacgtca
     3241 gcgccgttcc gccctatgtc cacgaatcgc cgtccactgt ctttccgcca ctgttccccc
     3301 tgctcagcga actgctggaa gcaagcctgc cgtcacgggt gatcgcgctg gatctgggat
     3361 ccctgccggg caaccgctgg aaagccgacc tgcacgatcc gcgcctgcgg gaagaggccg
     3421 atttttacct gtccgttcgt tcctcactgc ctgcgcatca ggtactgcac cagctaccgc
     3481 tggtctgcaa aatcggtgcg cctgatgacg tcacgctgct tatcaacgtg gcgctgaatg
     3541 gtgttccact ggtgccactg acctcagttc cggcagcgct gccactgcgc ctggagaatc
     3601 agtattttgc gctggatatg cacagtgacg cggctaaatc gatgctggag tccgggtgct
     3661 gcatgattta tgcgccagga accatgggcg acctgaaacc tgaactgttt gcggtgctgc
     3721 gcacatgaaa aaagagacgg atatcaatat cgacgcgctg ctgcgcgaca cgtttctgac
     3781 ggtggtggaa ctgcgtcagg gaaccaccgt gcgtcacggt atggagctgt atcgccactg
     3841 ccagcggcag gtggaggggg tccgcgaacg cctgaaagat gccgggttca gccgggagaa
     3901 cgtggagcac attacttacg cacagtgcgc cctgctggac gaaacagtcc tgagtcgcag
     3961 cggcatggac gacggtcagg ccatctggat gaaggatcca ctccaggcgc atttcttcaa
     4021 caccctgcag gcaggcgagc tgctgtacga gcggatgaag caggtgttgc aggagcccgc
     4081 gcctgcacag gcagtgctga cctgctttca ccgtgtcctg ctgctgggat ttcgcgggcg
     4141 ttatcaggat ccggtggccc cggagcgtga ccaacttatt tcgacgctca acgggctggt
     4201 cgcgccattt ggcgtgctgc cggaaacggc ggtactgaac gtgccgttat ccccccgtca
     4261 gcaccctttg ctgcattcgc cgtttttctg gctggtaacg ctgacgctgc tactggccgg
     4321 tgtctggtgg ggactgcacc actggctgaa cgtgctggtg gatgaattgc tgccgcaaag
     4381 cctgcggtaa agggagtcgt gagtgaggaa cacgctgaaa caggccgtcg tgctgtgggg
     4441 aatggtgtta ctgctggtgc tgtggtcggt gtttatcagt ccgtctggcg tactgatatg
     4501 ggccggggcg gcggctatcg ttctgacggt tgccgcgttg ctgatttacc ggcgcaggca
     4561 ggcgtggacg gagatgaccg gcgatgccgg gttgttatcg ctgccgccgg aaacgtaccg
     4621 acaaccggta gtgctggtct gtggcgatat gtcggcgcat ctgttcactg acagcccggt
     4681 ccggcaggtt tcagaagggc tgtatctgca tgtttctgat gaagaacagc ttgtggcgca
     4741 ggcggagcga ttactgaccc ttcgcccggc gtgggcatcg cagcttgccg tggcatatac
     4801 cgtcatgccc ggcatgtacc gggatgcggc ggttctgacc ggacaactgc gccggttcgc
     4861 ccacagtatg gcgacagtgc gtcgccgggc gggcgtaaac gtcccctggc ttctctggag
     4921 cgggctgtcc ggctcgccgt tgccggagag agcgaattca ccgtggttta tctgtaccgg
     4981 cggcgaaatt catgtagcga cgtctgctga aaccgcctcc cccgcgcagt ggctcacgca
     5041 gaccagcaca caggagcgca gccagcaact ctgttacctg ctgaaagccg aaagcctgat
     5101 gcagtggctg aatcttaata tgctggcggc actgaacggc ccggaaacga aatgtccgcc
     5161 actggcgatg gccgtggggc tggtcccctc gttgcctgcg gtggataaca acctgtggca
     5221 gttgtggatc accgccagaa ccggcctgac gacggatatc gcggacaccg gcacagacgc
     5281 cacgctgccg tttccggatg ccctgttacg gcggttgccg cgtcagtcgg gctttacccc
     5341 gctgcgacga gcctgcgtga ccatgctggg catcaccacc gtggcgggta tcgccgcgct
     5401 gtgtctgtca gccacggaga atcgccagtt attacggcat atcggtgatg atctgcacca
     5461 gttttatgcc gttccggcgg aggagtttat caccaaagcc cgtcgtctgt cggtgctgaa
     5521 agacgatgcg atcatgctcg atgggtatta ccgggaagga gagccactgc gtctcggtct
     5581 ggggttatac cccggtgaac aaatccgcca gccggtatta cgcgccattc gtgactggcg
     5641 tccgcctgag caaaaaatgg aagtgacagc ctcgcttcag gcgcagaccg tgcgtcttga
     5701 cagtatgtcg ctgtttgacg tcggacaggc ccgcctgaaa gacggctcga caaaagtgct
     5761 ggtggacgca ctggtgaaca tccgggcaaa accgggctgg ctgatcctcg tggccggata
     5821 taccgacgcc accggcgacg aaaaaagcaa tcagcagtta tcgctgcgac gtgccgaagc
     5881 ggtgcgcaac tggatgctgc agaccagcga catcccggcc acctgttttg ccgtacaggg
     5941 actgggcgag agccagcctg cggcgaccaa cgatacgcca cagggccggg cagtcaaccg
     6001 gcgcgtcgaa atcagtcttg ttccgcgttc tgacgcctgt caggacgtga aataaaacat
     6061 accgccggaa gaaggcggtg cttcaatcac actaacaagg agagtaattc tcatggctat
     6121 tcctgcttat ctctggctga aagatgacgg cggcgcggat atcaaaggtt ccgtggacgt
     6181 tcaggggcgc gaaggcagca tcgaagtggt ggcgctggat cacgatgtgt acatcccgac
     6241 cgacaacaac accggcaaac tgaccggtac tcgcacccat aaaccgttca tttttaccaa
     6301 agaaatcgat gcgtccagcc cgtatctcta caaagctgtg accaccggtc agaccctgaa
     6361 aacggcggaa tttaagtttt accgcatcaa cgacgccggt caggaagtgg agtacttcaa
     6421 catcacgctg gataacgtca agctggtcag agtcgctccg ctgatgcacg acatcaagga
     6481 gccttccaaa gagaagcata accacctgga acgcattgag ttccgctacg aaaaaatcac
     6541 ctggacttac aaagacggca acatcattca ttccgactcg tggaatgaac gtccttccgc
     6601 ctgataactg cggcagacag gaaactgtct gcctttctgt tgtgtgcttt gcccagtgcc
     6661 gcaggcaccg gtactgagca aagcataccc acaatctggt gatacctgat gggcctgagt
     6721 gaggtaacgg ctatgggcgg tagcgtacct gaacgcagat cgcaggaata cagaacacga
     6781 taaggaaacc ttctatgacc ggaaatcacc ccgccgcgct gctgcgtcgc cttaacccgt
     6841 actgtgcacg ggcgctggac gctgccgcct cactgtgtca gacccgcgcc catgcggaga
     6901 taaccattga acactggctg ctgaaactgc tggagcaggg agaaggcgat atcacggtga
     6961 ttgcccgccg ctatgaatgg gatatcgaca cgctctggca gtctctgctg gcacatctgg
     7021 acaccttacc ccgctcggtc cgcgaacgtc cgcagctttc tgaacccctg acggcgctta
     7081 tcaggcaggc gtggctgatt gcctcgctgg aaggcgacga cccgcaaatc cgcagtcagc
     7141 acctgctgat ggcgctgaca gaaaaaccga tgctgcccgc ctgtaatgac ctgtgggtat
     7201 tgctgagcct gagccgcgtg cagcttgagc ggctgcgccc cctgctggat gcgcagtcgg
     7261 atgaatgtcc ggcacgtcag ccacaggcca ccgaaccgct gacatccgcg caaccagagt
     7321 cggcaacgac ggacgcaccg gcaaaaacgc tgacggggaa acaggatgac gccctgctgg
     7381 cggtacttaa ccgctttacc gaagacgtga cggaaaaagc ccgcagcggg cgaatcgacc
     7441 cggtattcgg gcgcgacacc gaaattcgcc agatggtcga tatcctctcc cgtcgccgca
     7501 aaaacaaccc gattctggtg ggagaaccgg gcgtgggcaa aaccgcgctg gtggaagggc
     7561 tggcgctgcg tatcaccgaa ggcaacgtgc cggacagcct gaaaacggtg catatccgca
     7621 cactggacct cggtctgtta caggctggcg cgggcgttaa aggtgaattt gagcagcgac
     7681 tgaaaaacgt gattgatgca gtacagaaat caccggagcc ggtactgctg tttattgatg
     7741 aagcccatac cattatcggc gcgggtaatc aggcgggcgg cgcggatgcg gcgaacctgc
     7801 tgaaaccggc gctggcaagg ggcgagctgc gcaccatcgc cgccacaacg tggagcgaat
     7861 acaaacagta ttttgagcgc gacgccgcgc tggaacgccg cttccagatg gtgaaagtcg
     7921 acgagccgga tgatgacacc gccagcctga tgctgcgggg actcaaggcc cgctatgcac
     7981 agcaccacgg cgtacatatg ctggacagcg ccattcagac cgccgtgcgc ctgtcgcgcc
     8041 gctatctgac cggacgccag ttgccggaca aggcggttga tttgctggat accgccgggg
     8101 caagagtccg catgagcctc gacaccctgc cggaaccgtt gacgcagctt caggcgcgtc
     8161 tgaccgcgct ggatattgag cgggaagcga ttgagcagga cagcgtgttt tatcccgaaa
     8221 ccagcccgga gcggctggcg gaactgaccg atttgcgtga tgagctacag gcagaagccg
     8281 ggcatctgga agcgcagtat cagcaggaaa agcggcttgc gcagcagatt atggcgttgc
     8341 gtcaggaagg ggcagacagc actgatttgc aacagcaact gcgaacgcat cagggctttg
     8401 caccgctgct ggcgctggat gtggacgccc gcgccgtcgc cactgtggtg gcggactgga
     8461 ccggcatccc gctctcttcc ctgctcaggg acgagcagag cgacctgctc agtatggaac
     8521 aaagccttga aaaccgcgtt gtcgggcaaa gcccggcgct ctgcgccatc gcacagcggc
     8581 tgcgggcggc taaaaccggc ctcacgccgg agaacggccc gcagggggta ttcctgctga
     8641 ccggccccag cggcaccggt aaaaccgaaa ctgcgctcac actggccgac accctgtttg
     8701 gcggtgaaaa atcccttatc accatcaacc tctccgaata tcaggagccg cataccgtct
     8761 cccagcttaa ggggtctcct ccgggctacg ttggctacgg tcagggcggc gtactcaccg
     8821 aagccgttcg caaacgcccg tacagcgtgg tgctgctcga cgaagtggaa aaggcgcatc
     8881 gcgacgtgat gaacctgttc tatcaggtat ttgatcgggg ctttatgcgc gacggcgaag
     8941 gccgggaaat tgacttccgc aacaccgtga ttctgatgac ggctaatctg ggcagtgacc
     9001 acatcatgca gctgctggag gaaaaaccgg acgccacgga cgcagacctg catgaactgc
     9061 tgtaccccct gctgcgcgag cacttccagc ctgcactgat ggcgcgtttt cagacggtga
     9121 tttaccgccc tctgggacag gaagcgatgc gcgccattgt ggaaatgaaa ctggcgcagg
     9181 tggttcgtcg tcttcgtcag cactatggaa atcaacgaca gcctgtatga cgcgctgacc
     9241 gccgcctgcc tgctgccgga caccggggcg cgtaatatcg acagcctgct gaaccagcaa
     9301 atcctgccgg tcttaagcca gcagttgctg gcgcaacagg ccgcgcaccg taaaccagcc
     9361 cagctacggc ttggctggga tgaggaagac gggattgtac tggagttcgc tacggaagag
     9421 atgcaataac ggaggctgtc atgggtattc agaatttcga tcattcacac cacaagctca
     9481 aaattcgtgg cctgcaaagt ccggtggatg tgctgacctt cgaaggccgc gaacaactga
     9541 gtaccccgtt ccgctacgac attcagttta ccagcagcga taaagccatc acgccggagt
     9601 cggtgctgat gcaggacggc gcattcagcc tgacggcacc gccggtacag gggatgccgg
     9661 tacagactgc cctgcgcacc ctgcacggcg tcatcaccgg ctttaaacac ctgtcatcgt
     9721 cacaggatga agcccgttac gaagtgcgcc ttgaaccccg aatggcgctg ctggcccgca
     9781 gccgccagaa cgccatttac cagaaccaga cggtgccgca aatagtcgaa aaaatcctgc
     9841 gcgagcggca ccagatgcgc ggtcaggact tcgtgtttaa cctcaaaagc gaatacccgt
     9901 cccgtgaaca ggtgatgcag tacggtgagg acgacctgac gtttatttca cgcctgctgt
     9961 cggaagtggg tatctggttc cgctttgcca ccgacgcccg gctgaaaatt gaggttgtcg
    10021 agttttacga cgaccagagc ggctatgagc gcggactgac gctgccgctt cgccatcctt
    10081 cagggctgtt tgatggtgaa acggaagccg tatgggggct gaacaccgca tacagcgtgg
    10141 tggaaaaaag cgtcaccacg cgggactaca actaccgcac cgccacagca gagatgatga
    10201 cagagcagca tgacgccacc gggggcgata acaccaccta tggcgaagcc taccactacg
    10261 ctgacaactt cctgcaaaaa ggcgacaagg aggcagcgga aagcggcgcg ttttatgccc
    10321 gtatccgtca cgaacgctac ctgaatgaac aggccattct gcagggccag agcaccagtt
    10381 cactgctgat gccggggctg gaaatcaggg ttcagggcga cgacgcgcct gcggtattcc
    10441 gcaagggcgt gctgattacc ggcgtgacgg ccagcgcggc ccgtgaccgc agctatgaac
    10501 tgacgttcac ggcgataccc tactcagaac gctacggcta tcgcccggcg cttatccccc
    10561 gccccgtaat ggccggaaca ctcccggcgc gggtcaccag tacggtgaag aacgacatct
    10621 acgcccatat cgataaggac gggcgctacc gggtcaatct cgactttgac cgtgatacgt
    10681 ggaagccggg ctacgaaagc ctgtgggtac gccagtcgcg cccgtatgcg ggagacacct
    10741 acggcctgca cctgccgctg ctggcgggaa cggaagtcag catcgccttt gaggaaggca
    10801 acccggaccg accgtatatc gccggggtaa aacacgactc ggcacacacc gaccatgtga
    10861 ccattcagaa ctacaagcgc aacgtgctgc gcaccccggc gaacaacaaa atccgcctcg
    10921 atgatgagcg cggcaaagag catatcaagg tcagtaccga gtacggcggc aagagccagt
    10981 tgaatctggg tcatctggtg gatgccggaa aacaacagcg cggggaaggc tttgaactgc
    11041 gtaccgatat gtggggcgcg gtgcgggcga agaaagggat ttttatcagc gccgatgcgc
    11101 aggataaggc gcaggggcag gtgcgcgaaa tggctccggc gatggcaata ctggatggcg
    11161 cacagtcaca gatgcagtcg ctgtccactg atgcgcagac gacgaatgcc gatccggcag
    11221 atctgtcatc gcagatagcg ctgttgcagc aaagtgtaaa agatctgacg caggccgtga
    11281 tcctgctaag tgcacccaag ggcgtggcga tcgccagcgg cgagcacctg caactggcgg
    11341 cgtcgaagaa tgtgatagcg aacgcaggca acaacgcaga tatcggcgta gtaaaaaata
    11401 tgtttatcgg tgtgggtcag gcgctgagcg tgtttgtgcg taaagcgggc atcaagctgt
    11461 ttgccaataa aggggcggtc tcggtacagg cacagaacga tctgatggaa ctgttggcac
    11521 agaaatcgat agagatcacc agtacggaag atgagatcaa gatcaccgcg aagaagaaaa
    11581 tcacccttaa cggcggtggc agctacatcc ggctggatgc ctgcgggata gaagcgggaa
    11641 cgccgggaga atataacgtt aaggccggat attacgggcg taaaccaaag gcgaagctga
    11701 cgccggagct gatggcattt ccggtgatta agtctgagga ttttaatcag agctttattt
    11761 tgctggatga aaatactggg caaccactta ttaattggcc ctatgaacta gaactggaaa
    11821 gtgggctaaa aatgagtggg attactgatg aaaatggaaa tacagaacta atatcctctg
    11881 ataaggagga ggtcgttaat attagtgtgt ttgaacccga tgagttttta gacgatgaca
    11941 ttaattaaga catatagcga gaatataaca tggcagataa atccgaaaag aaaaaagaaa
    12001 ccacaaataa atcagcaaaa aaaacactaa cgaagaagag cgaaaaatta actccagcaa
    12061 aagacaccgc accaaaacaa gtgaatgtag atccagctat aagattgaaa caaattcgtc
    12121 aacttgtccg taataataat aaatc
//
LOCUS       DAZFWP020000144        11986 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFWP020000144 DAZFWP020000000
VERSION     DAZFWP020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.144"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            114..229
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     rRNA            114..229
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            555..1853
                     /gene="kgtP"
                     /locus_tag="ACHK0Z_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             555..1853
                     /gene="kgtP"
                     /locus_tag="ACHK0Z_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4503836.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1850..2173)
                     /gene="yfiM"
                     /locus_tag="ACHK0Z_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             complement(1850..2173)
                     /gene="yfiM"
                     /locus_tag="ACHK0Z_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU4503837.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2219..3574)
                     /gene="pssA"
                     /locus_tag="ACHK0Z_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             complement(2219..3574)
                     /gene="pssA"
                     /locus_tag="ACHK0Z_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU4503838.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3688..6348)
                     /gene="pat"
                     /locus_tag="ACHK0Z_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     CDS             complement(3688..6348)
                     /gene="pat"
                     /locus_tag="ACHK0Z_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU4503839.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6380..7078)
                     /gene="tapT"
                     /locus_tag="ACHK0Z_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6380..7078)
                     /gene="tapT"
                     /locus_tag="ACHK0Z_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU4503840.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7147..7566)
                     /gene="trxC"
                     /locus_tag="ACHK0Z_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     CDS             complement(7147..7566)
                     /gene="trxC"
                     /locus_tag="ACHK0Z_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU4503841.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7773..8810
                     /gene="yfiF"
                     /locus_tag="ACHK0Z_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             7773..8810
                     /gene="yfiF"
                     /locus_tag="ACHK0Z_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4503842.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8858..9547)
                     /gene="ung"
                     /locus_tag="ACHK0Z_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             complement(8858..9547)
                     /gene="ung"
                     /locus_tag="ACHK0Z_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU4503843.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9852..10235
                     /gene="grcA"
                     /locus_tag="ACHK0Z_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             9852..10235
                     /gene="grcA"
                     /locus_tag="ACHK0Z_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU4503844.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10291..10878)
                     /gene="eamB"
                     /locus_tag="ACHK0Z_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             complement(10291..10878)
                     /gene="eamB"
                     /locus_tag="ACHK0Z_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU4503845.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10981..11862
                     /gene="yfiE"
                     /locus_tag="ACHK0Z_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             10981..11862
                     /gene="yfiE"
                     /locus_tag="ACHK0Z_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4503846.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2986 a         2993 c         3106 g         2901 t
ORIGIN      
        1 aaccgtgagg cttaacctta caacgccgaa gctgttttgg cggatgagag aagattttca
       61 gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg
      121 gccgtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg
      181 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaattaag
      241 cgaaaggcca tcagtcacga tggccttttt gcgttggcgc agaaaaaaat gcctgatgcg
      301 acgctgcgcg tcttatactc ccacatatgc cagattcagc aacgaatacg gcttccccaa
      361 cttgctcact tccatacgtg tcctccttac cagaaattta tccttaagct cctcaataat
      421 cattttcctg ctaactaaat tcatggttaa ggttgcataa tgatatgcaa caaatgtata
      481 acattttctt taccaaaaga aataaacaaa agtgaccgac aaaagcatcg gattacggca
      541 ggagacataa tggcatggct gaaagtactg taacggcaga cagcaaactg acaagtagtg
      601 atactcgtcg ccgcatttgg gcgattgtgg gggcctcttc aggtaatctg gtcgagtggt
      661 tcgatttcta tgtctactcg ttctgttcac tctacttcgc ccacattttc ttcccttccg
      721 ggaacacgac gactcaacta ctacaaacag caggtgtttt tgctgcggga ttcctgatgc
      781 gcccaatagg cggttggcta tttggccgca tagccgataa acatggtcgc aaaaaatcga
      841 tgctgttatc ggtgtgtatg atgtgtttcg gatcgctggt tatcgcctgc ctcccaggtt
      901 atgaaactat aggtacgtgg gctccggcat tattgcttct cgctcgttta tttcagggat
      961 tatccgttgg cggagaatat ggcaccagcg ccacctatat gagtgaagtt gccgttgaag
     1021 ggcgcaaagg tttttacgca tcatttcagt atgtgacgtt gatcggcgga caactgctag
     1081 ccctactggt tgtcgtggtt ttacaacaca ccatggaaga cgctgcactc agagagtggg
     1141 gatggcgtat tcctttcgcg ttaggagctg tgttagctgt tgtggcgttg tggttacgtc
     1201 gtcagttaga tgaaacttcg caacaagaaa cgcgcgcttt aaaagaagct ggatctctga
     1261 aaggattatg gcgcaatcgc cgtgcattca tcatggttct cggttttacc gctgcgggct
     1321 ccctttgttt ctataccttc accacttata tgcagaagta tctggtaaat actgcgggaa
     1381 tgcatgccaa cgtggcgagt ggcattatga ctgccgcatt gtttgtattc atgcttattc
     1441 aaccactcat tggcgcgctg tcggataaga ttggtcgccg tacctcaatg ttatgtttcg
     1501 gttcgctggc agccattttt accgttccta ttctctcagc attgcaaaac gtttcctcgc
     1561 cttatgccgc ttttggtctg gtgatgtgtg ccctgctgat agtgagtttt tatacatcaa
     1621 tcagtggaat actgaaggct gagatgttcc cggcacaggt tcgcgcatta ggcgttggtc
     1681 tgtcatatgc ggtcgctaat gctatatttg gtggttcggc ggagtacgta gcgttgtcgc
     1741 tgaaatcaat aggaatggaa acagccttct tctggtatgt gaccttgatg gccgtggtgg
     1801 cgtttctggt ttctttgatg ctacatcgca aagggaaggg gatgcgtctt tagtgacggg
     1861 tcagttgcca gacggtatag ccggtgcttg caccggcgac atcccaggcc aaatccttcc
     1921 agctccagcc gctcccttcg gggcggctat cccaaagctc ttttgacgcc cccaaactga
     1981 cagagaacat caatccaaac atggcactgc gatcccggct catcccctga tgctgtgaat
     2041 attcatttcc ggcggcggaa agcatcgccg aggcgataaa gtgttgagct ttatcctgcc
     2101 cgctccagct atcgttagcc atatggctgc atccagaaag taacaatagc gaacagacaa
     2161 aaagaatacg caaaacgtgg cctccaaaaa acaaaccccg tacaggacgg ggttgtgatt
     2221 acaggatgcg gctaattaat cggtcgatgc ggatacggcg caaacggcgg atgagtttac
     2281 gaaccttcac cggataatcg gcaatacttt gcagatcgcg atagtgctta acgatggtgg
     2341 tatgctcgcg gatcagctcc agttctttct ctcgctgcgg cgcaagctcc agttgtggat
     2401 cgtggatcaa aatggcgttt tccagatcca gacgccaggc gcgcgggttc aggttattac
     2461 cggtgatcaa catccactta tcatcaaccc acatcccttt taggtgatag gtgttgtcgt
     2521 catctttcca taaccgaacc actagctggt cagtattgac gtaatactgc aaacggctca
     2581 ggaaacgacg cagattgatc tcatagagat aaggcaatgc gccaattatc ttgaaaggtt
     2641 catcttccgg aatgtagaag tcgttcgcgg ttttatcacc aacaataatt tcgacctttt
     2701 tcccttcgcg cagcaactgg ataatattgc gcacaaggat tgctggcagg ttgaagtatg
     2761 gcgtacagat ggttagtttc tgctcggcac aaggcataag atggaaaatg gtcttgttca
     2821 acagactcga tttccccagc cccactagcg gcgttacgga aagctgatcg ttgtcggcat
     2881 cgccctggaa atgataagcg gcatcacgca gctcctggcg gaacagacga atatcgttct
     2941 tgatttccgg gctttttggc cgattaacat catccagacg attaacgccg cggccattca
     3001 taatattctg tgtaacccat tcaaacataa tgtctgacat cttacggtta cggatcagat
     3061 gataacggtc gtagcgatat ttatcgtgct gatgcaggta aacatcgttc aggctggcac
     3121 cgctataaag tacgctatcg tcgatgataa agcctttaaa gtgcagaaca ccaagggctt
     3181 cacgggtatt gattggaacg ccataaaccg gaacatctac gcccggattt tcctgcgcca
     3241 tgcggcagta ccagtcagcg ttagtgttag atgccgcagc gccaatgcgt ccacgttgtg
     3301 cacgatgcca gtcgaccagc acccgcacat ccagttccgg acgctgcctt ttagcttcat
     3361 acaacgcgtt cagaatgcct ttgccaccgt catcctgttc gagatacagg gcgacaatac
     3421 aaatgcgctg cttcgcgctg gctatttttt ccagcagcgt ctcccggaag tcggcgggag
     3481 cgtaaaagaa atcgacatca tcaactgatt gagaaatctt gggtagttgg gcaaggtgtt
     3541 gttgatgttt attacgctta aattttgaca acatcacagt gcatttcttc tctgttcatt
     3601 gaagggtcct ctgtgcaatg cagacgacat aagcgggcaa taataacacc agtcccgatt
     3661 aagtggtcaa catttccagt accttactca tgattcctcg cgttgggcaa gatttagcgt
     3721 aagcccaacg atcccctctt cgagctggat atcaacgtta aacccgagct tgcgggctag
     3781 cgccaccatg ccacgattgt ttggcatcgt aataccattc agacgttgta gtccgtgatc
     3841 tcgcgtatag gtaatcaact tttccattaa gcgtcgacct aagcctaacc ctttgagatc
     3901 cgagcgaacc agcacagcaa attcggcatc gatgttatca ggatcggaaa tcgcacgcgt
     3961 gacgccgagg atctcttccg tttgatcaat acgtcgtacc gctacaaacg ccatttcccg
     4021 atcgtagtcg atctgtgtca tgttggctaa atcttcatgg gtaaattcgt tgatctcgct
     4081 aaagtagcgg taataaagat cttctttggt gactcgcgaa atgaattgct gaagttgtgg
     4141 ctcatcttct ggcaaaatcg ggcggaacaa gcagcgttca ccgtttttca attctaccca
     4201 ttcttccagc tgatgcggat aagggcgcac tgccagccga ctctcgttat cgccttcaaa
     4261 cggcgagata tccagcgtga catccagcgc ggtaaattca ctgccagaag ccagcaaagg
     4321 atgaatatcc agacgctgaa tttccgggca atcgacaatc aagttggaaa cctgcaccag
     4381 aagctggctc aagcctgcaa catccaatgg gcgtagcgca ctgcgcgcac gaatcttttt
     4441 acttttgatc ccctgaataa ccagatagcg ggccaggttc atgttcagcg gcggcagtgc
     4501 gacgacggct tgatcttcag gacgccactc cacaccgcct tcacccagca tgatcaacgg
     4561 cccgaaaacc ggatcgtgct caaccacaac ccgcaactcc tgagcgccag cacggttagc
     4621 catactttgc accaacaggc cgtggacccg cgcctgtggc caggccattt ttacgcgatc
     4681 gaaaatagcg ttcgccgctt gctggacttc attggctgta cgcaggtaaa gcatgacgcc
     4741 ctgaacttcc gatttatgtg gaatatccgg cgaacgcaat ttcagcgcca ccggataacc
     4801 aatctgttcg gcaatatgca ccgcttcggt gctatcgctg gcaatccagg tagggagcgt
     4861 gttcatgcca tacgcttgca ggatgggctg aacttcatgg gtatcgagcg acgtagcccc
     4921 ttcggcaatc gcctgttgca acagaagatg cgcttctgcg gtattggaag tcagattgct
     4981 gggcaacgcc ggcgtttcgc gtagttgctt ctgattacgc cggtactcca ccatatgcat
     5041 aaaagcagtg atggttcctt ccggggtacg gtaggtcggc agcccggctt cgctgaataa
     5101 tcgtcgtgcc tcttgcgagg aatgttcgcc acaccagttc gtcagcagag aaacatactt
     5161 gctgcgggga tgatgcttta ccgcttcaat taatacttgc gcgctttctg ttgcgggagc
     5221 agcggcgctg ggcgaatgaa taaccatcag cgcatcaaaa tcctggctgt ggagcagaat
     5281 gtccagcgtt ttaatatagt gctcactgct ggcgtcatcg cgcagatcga gcgggttaga
     5341 gattgccaca tgtcctggca gtgcatcgcg cagtttctgg caggtttctt cgcttagcgt
     5401 tgccagcttg ccattgcgtg accataaggc atccagcgcc agcgcggcag gcgcagcacc
     5461 gttgctgata atcatcaacc ggtcgccacg cagcgggcgc atatggctaa gggtttccac
     5521 cgccgaaaac agttcgtggg tatcctgtac ccgcaacaaa ccggcacgct gaatagccgc
     5581 atcccatgcc gggtccattc ctgccgtcgt gttgagcagt cgctgtgccg ccgggctacg
     5641 tccgctttta atcaccagaa tcggtttatt acgcgaggca ctacgggctg ccgaaacaaa
     5701 gcgtcgcgcg tcgcttaact gttcgagata gagcaggatg gcgctggttt tactgtcgcg
     5761 cgccagatag tcgagcaatt catcaacgtc gatatccagg ctgtcgccga gcgcaataaa
     5821 gtaggaaaag cccatctcac gctgttgcgc ccagtcgagg atggtgttgg agacggctgc
     5881 cgattgtgaa ataaacgcca gcttgccgcg tttaatcggc acaggcgaaa agctggcatt
     5941 cagaccttgc cagggagcca gtaaacccag actgtttggt ccaagcaggc gcatgttatg
     6001 gcgcagggcg caggcgcgga gatcttcgtg ttgcgatgcc ggggcggaaa gaataatgca
     6061 ggttttacag cctttctcgc cgagctcttc cagaagagca agattacggc tggcattggt
     6121 acataaaacc gcaaggtcgg gtgtaaaggg caagctggca atatccggcc aggccaacac
     6181 acccaacacc gctttccagg ctggcgtcac cgggagtacc ggtccgttaa agcctcccgc
     6241 cagcaggtta cgcatcatca ggtaacctgc gcgattgggt ttcatcgacg cgccaattac
     6301 cgctatcgat tttggtcgca gtagtgcttc cagtcctcgc tgactcatac cggtctcccg
     6361 cagaagtgac cgaatgattt taaacgcttt ctaactgttc tgctgtgatg ctacccagat
     6421 gttgcgtttt tcctgccaga tagcgtgttt taaagcgggt aaaatgctcg cctaaccctg
     6481 ctgccgcccc ggtatcgctg gccatatcta acagtgcgat ggctacctcg gcagtacaat
     6541 attggcctgc agcctgggct tcacgcaggc gataggcaga aagccgggaa agatcgacgg
     6601 aaatgacggg aagattatcc agatacggac ttttacgaaa catcttgcga gcttccggcc
     6661 aggtaccatc gagcatgata aacagcggtg gcttaccggc aggtggtgtg aagatcactt
     6721 cccgttgctc atcagcatac gaagcgggaa agaccaccat tggctgatag tccgggtttt
     6781 gcaccaactc cagcaaatcc tgcgagggtt cggtacgcga ccattgaaac gcaacggtat
     6841 caggcaaaat atcagcaatc agacgcccgg tattactggg cttcattggc tcggtgtcga
     6901 acatcagcaa acagaagcga ctttttgctt gtgctggggt aattgtcgaa cagagacata
     6961 atttctctgg caaaagacag cgttggcagc gacgaacgcg attaccgcgg gcaagaaaag
     7021 gacgtgttgc gcgcgcaata cgctcggcgc gtaactggag aacagcgttt tcggtcataa
     7081 gagagcgtcg aaaaaacgcc attgtcgcag aggagaaaac ggggcacaag atgcgccccg
     7141 gtaagattaa agagattcgt tcagccagct atcgaacggc gctttcggta ctgcgccatt
     7201 aagcatgtcg acaacctgac cgtttttgaa aatcatgatc gtcggtatac tacgaattcc
     7261 aaagcgactg ctcaattcac gttcagcttc ggtattcact ttcacaaagc gcactttacc
     7321 gctacgctct tgcgcgacat cttcaaaaat tggtgcgaaa ttacggcagg ggccgcacca
     7381 cggtgcccag aagtcgatca ccacaggtag atcatccttc agcaatttgt cgagtgtttc
     7441 accggtcgca ttaatcacct ctccgtcaaa caagtcgtga ccgcagcgtc cgcattttgc
     7501 cgcatcttcg atccgatcgt cgggaatgcg attgatggcc tgacaatggg tacaaacggt
     7561 attcataact aacctcggga tgagtaagga gacttatcgg caacgacgcc ggtatgtttc
     7621 taatatgtta catattatcg gggacattgt tccagacaac aatctgtttt attcaatagg
     7681 tatgatagtc gcaggctttg gtacgaaatc atggcgaacc gcgcggactt cgggtaatct
     7741 gcgcgcttcg cgcagcgctg gtggagaaaa gcatgaacga tgaaatgaaa ggtaaaagcg
     7801 gcaaggtcaa agtgatgtat gtccgcagtg atgatgattc tgataaacgt acccacaacc
     7861 cgcgtaccgg gaaagggggc gggcgtccag gaaaatctcg tgctgacggt ggccgtcgcc
     7921 ccgcccgcga tgacaaacag agtcagcccc gtgaccgcaa gtgggaagat tcgccgtggc
     7981 gcacggtttc ccgcgcgccg ggtgatgaga cgccggaaaa ggccgatcac ggtggcatca
     8041 gtggtaaaag ttttattgat ccggaagtgt tgcgtcgtca gcgtgcggaa gaaacccgcg
     8101 tctacggcga aaatgcatgt caggcacttt tccagagccg tccggaagcg attgttcgcg
     8161 cctggtttat ccagagtgta acgccgcgtt ttaaagaagc cttgcgctgg atggcagcaa
     8221 accgcaaagc gtaccatgtg gtggatgaag cggaattgac aaaagcgtca ggcacggaac
     8281 atcacggcgg cgtttgcttc ctgatcaaaa agcgtaatgg cacaaccgtg cagcagtggg
     8341 taagccaggc aggcgcgcag gattgtgtac tggcgctgga aaacgaatct aacccgcata
     8401 acctgggtgg catgatgcgc agctgcgcgc acttcggcgt gaaaggcgtt gtggtgcagg
     8461 atgcggcact gctggagtcg ggggcggcta tccgtaccgc agaaggcggc gcagagcacg
     8521 ttcagccgat tactggcgac aacattgtta acgtgttgga tgatttccgt caggcaggtt
     8581 acaccgtagt gaccacttcc agcgagcagg ggaaaccgct gttcaaaacc agtctgccag
     8641 cgaaaatggt actggtgctg ggtcaggaat atgaagggtt accggatgcc gcacgcgatc
     8701 cgaacgatct gcgcgtgaag attgacggta cgggcaacgt tgccgggctg aatatctctg
     8761 ttgcaaccgg cgttttgctt ggtgaatggt ggcgtcagaa taaagcctga taaatcagcc
     8821 gggtggcaac tctgccatcc ggcattttac cgcaaattta ctcactctct gccggtaata
     8881 ctggcatcca gtcaatcggc gtctcgccac gttgttccag ccactgattt gccagcacaa
     8941 aatggttgca gccaaagaat ccacgatgcg ccgaaagcgg cgacggatgc ggtgctttca
     9001 gtacatgatg gcgttgctta tctataatcg cccctttctt ttgcgcatgt gatccccaca
     9061 acaaaaacac cacgccttcg cggtgctggt taatcaggct gatgacttta tcagtaaacg
     9121 tctcccaacc gaggctggcg tgggaatgcg cctgacctgc gcgaaccgtc aacacagtat
     9181 tgagcagcag aacgccctga cgcgcccagc tttcgagata gccatgatta gggcgggtga
     9241 agcccggaat agtattttcc agctctttat acatattcaa taatgacgga ggaatggcaa
     9301 tgccgggacg aacggaaaat gccagaccat gcgcctgtcc cggtccgtga taaggatcct
     9361 ggccgagaat caccacttta acgtcaccca actcggtaaa gcggaacgcg ttaaagacat
     9421 ctttttgtgg tggatagata gtgacgccgg actgccgctc gctggcgacg gtctgaaggg
     9481 tattaagaaa ataaggttgt tgcttctctt cagccagcac gtcatgccag gttaattcgt
     9541 tagccatctc gctctcctgc gaatcttcaa tccgcctagc ttaactgctt ctttctacag
     9601 aacaaaatca tgctcatttt ttagacttaa acgagtgcaa aaaaatgtaa attggtaaaa
     9661 tttcacaccc gcataagttg atgtaaaaca ataaaatcca tttacgacca caatcgatgt
     9721 gtggttttta ttgatttaaa tcaaagaatc aagggtggtt gaggagtata tatacactca
     9781 agcaacaatg gttttaccaa ttggccgcga caggctgaac aaatcaaata attttgccgg
     9841 ggaggcatca catgattaca ggtatccaga ttactaaagc cgctaacgac gatctgctga
     9901 actctttctg gctgctggac agcgaaaaag gcgaagcacg ttgcatcgtt gcaaaagcag
     9961 gttatgcaga agatgaagtg gttgcagtaa gcaaactggg tgacattgaa taccgtgaag
    10021 ttccagtaga agtgaaacca gaagttcgcg ttgaaggtgg tcaacacctg aacgttaacg
    10081 ttctgcgtcg tgaaactctg gaagatgcag ttaagcatcc ggaaaaatat ccgcagctga
    10141 ccatccgtgt atccggttat gcagttcgct ttaactctct gactccggaa cagcagcgcg
    10201 acgttatcgc tcgtaccttt actgaaagcc tgtaaggctt aagtagcaag ttactaagcg
    10261 gaagagggcg acctcttccg ctttttttcg ttaatagaaa atgcgtaccg cgcaatagac
    10321 cagcaacagg gcaagcacga tatttaactg gcgaccatac tggcgaaaca atcgctgaaa
    10381 cagatgcccc gccagcgccc agcacacatt gccaaacgtc ccaatcatcg ccagcaaaac
    10441 gctgacgcca actacccagc ttagcgcctg tgtttgcggc agaacaaacg tcgacagtgc
    10501 cgtaacaccg tacaaaatga ttttgacgtt cacaaactgc aaagcaaagc tggcccaaaa
    10561 gctgattggt tttgcctgaa gtccgtcttc ctttgttggg ctggtggcga ttttccacgc
    10621 cagccagaca atatatgccg cccccgccca actcaaaagg tgtaccgctg ccgggtcaat
    10681 cactgccagt gaaaatgaaa tgcccgcaca cagtaacatc acaatcaaaa atcccagact
    10741 catccctgcc agcacgcggg tactttgacg aaatccatgc gacgtagcag agctaagggc
    10801 gagaatattg ttcggtcctg gcgtcatagc ggtaatcagg gtgtaagtcc aaaaagcact
    10861 taaaagggtc ggtgtcactt gttatctcct ctgttttggt agacagtacc cgacacggaa
    10921 catttaaaaa aatgaatgtt tttatattct agattcgata ttttcgatag gtttggggtt
    10981 atggatctgc gccgttttat tacgcttaaa accgtggtgg aagagggttc ctttttgcga
    11041 gcttcgcaaa aattgtgctg tacacaatcg acggtgactt ttcatattca gcagcttgag
    11101 caggagtttt cagtccagtt atttgagaaa attggccgac ggatgtgcct tacccgcgaa
    11161 ggaaaaaagt tgctgccgca catttatgaa cttacccgag tgatggatac gcttcgtgag
    11221 gctgccaaaa aggagtcaga tccggacggg gaactacgcg ttgtttcggg cgaaacgttg
    11281 ctctcttacc gaatgccgca ggtgttgcag cgttttcggc aacgcgcacc aaaagtgcgt
    11341 ttatcgttgc agtcgctgaa ctgttacgtg atccgtgatg cgctactgaa tgatgaggcc
    11401 gatgtcggcg tcttttatcg cgtagggaat gatgatgccc tgaaccgacg agagttgggt
    11461 gaacaatcgc tggtgctggt ggcttcaccg caaattgcag atgtcgattt tactgagccg
    11521 ggaagacata acgcctgtag ctttattatc aacgaaccgc aatgtgtctt ccggcagata
    11581 tttgagagca cgctgcgtca gcggcggatc acggtggaaa acaccattga gctgttaagc
    11641 attgaaagta tcaaacgttg tgtcgcggcg aatatcggtg tcagttactt gccccgcttt
    11701 gcggtggaaa aagagcttga gagcggagaa ttaattgaat tgccttttgg cgaacagtcg
    11761 cagaccatta cggccatgtg cgcacaccat gctggaaaag cggttagccc ggcgatgcac
    11821 acttttattc agtgtgttga agagagtttt gtggctggat aaaaataatg tcgggattaa
    11881 acccggcatt taacttgtcg acaaagtgcg ctttattcat gccggatgcg gcgtgaacgc
    11941 cttatccggc ctacaagatc gtgcaaattc aatatattgc atgagc
//
LOCUS       DAZFWP020000145        11921 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFWP020000145 DAZFWP020000000
VERSION     DAZFWP020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.145"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..501
                     /locus_tag="ACHK0Z_RS19525"
                     /old_locus_tag="pgaptmp_003905"
     CDS             <1..501
                     /locus_tag="ACHK0Z_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HGU4503847.1"
                     /translation="DFRFLHPDFNYIYQNYGDDFVTHTAKFSGETVERAIERAIVSKL
                     NIVVEGTFRNAATPLQTLKKLKDAGYQTEVMIKTTSAALSWESTNERYSKDKEAGNIA
                     RKVDKNHHDIVTGLLAENARKVFASNLSDKFAVYSREKMIFSSQAATNDDIATLIQNE
                     ISGNTQ"
     gene            complement(537..1328)
                     /locus_tag="ACHK0Z_RS19530"
                     /old_locus_tag="pgaptmp_003906"
     CDS             complement(537..1328)
                     /locus_tag="ACHK0Z_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGU4503848.1"
                     /translation="MPLNGVSAAMRERVSQQLREIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1334..2461)
                     /locus_tag="ACHK0Z_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             complement(1334..2461)
                     /locus_tag="ACHK0Z_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HGU4503849.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHILPWFTTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVERYCVVADLSETEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            2990..3262
                     /locus_tag="ACHK0Z_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             2990..3262
                     /locus_tag="ACHK0Z_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU4503850.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDHARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAAAKKARQLKKKQAYKPDTEASFNWSANTPTHGRR"
     gene            complement(3263..4135)
                     /gene="rluF"
                     /locus_tag="ACHK0Z_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             complement(3263..4135)
                     /gene="rluF"
                     /locus_tag="ACHK0Z_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU4503851.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPLVKMEKTSEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            4347..5036
                     /gene="pepE"
                     /locus_tag="ACHK0Z_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             4347..5036
                     /gene="pepE"
                     /locus_tag="ACHK0Z_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU4503852.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5127..6758)
                     /locus_tag="ACHK0Z_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             complement(5127..6758)
                     /locus_tag="ACHK0Z_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU4503853.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGGEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6978..10661)
                     /gene="metH"
                     /locus_tag="ACHK0Z_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             complement(6978..10661)
                     /gene="metH"
                     /locus_tag="ACHK0Z_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU4503854.1"
                     /translation="MSSKVEQLRAQLNQRILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            10861..11685
                     /gene="iclR"
                     /locus_tag="ACHK0Z_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             10861..11685
                     /gene="iclR"
                     /locus_tag="ACHK0Z_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU4503855.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDSVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2864 a         3265 c         2924 g         2868 t
ORIGIN      
        1 gattttcgat ttctacaccc tgattttaat tatatctacc aaaattatgg ggatgatttt
       61 gttacccata cagcgaaatt ttccggcgaa acggtcgaac gtgcaattga gagagcgata
      121 gtcagtaaac tcaacattgt tgtcgaagga acatttcgta atgccgcgac gccgttacaa
      181 acattaaaaa aactcaaaga tgcaggctac caaacagaag tcatgataaa aacaacctca
      241 gcggcattaa gctgggaaag caccaatgaa cggtatagta aggacaaaga ggcgggaaat
      301 attgcccgaa aagtggataa aaaccaccat gatatcgtca ccggtttgct ggcagaaaat
      361 gcccgcaagg tgtttgcttc gaacctgtca gataagtttg cggtgtattc gcgtgagaag
      421 atgatattta gttcacaagc ggcaacgaat gacgatattg caacacttat acagaatgaa
      481 atcagcggga atacacaata ataataagag ctgatggcgt gaaataacgc cgggctttat
      541 gcccggcgca ttaccgtctg gtacattaag ctatccagtt ccttaacgtc cccttctctg
      601 ctatccggta acagcggtgg aatttcccca cgtgcctgtt ccgcacgaat aaacgcatgt
      661 aacaatggac ggcgcggacc atattccgct tctcctgcgc gctgtttacg ttccagtaat
      721 tcgtctattt ctgcacgtaa cggcgcatca agttcgcttc ccgccagtaa ctcagcaaag
      781 cgcattggcg gtacgccttt acccgcttcg acccagcgca cggccagcag cggacgcagc
      841 acgtaaaagt attttttcag ccgcacttcg tcgccttgta aataaccgcg aaagtttttc
      901 cgcgccatcg aataataatg ccaacgcgca cgcagcggag aaaaccactt cggcaccatt
      961 gccttaagcg ccgttatcgt ttcttcatct tgttgataga ccacaggaga atccagccat
     1021 tctatcagcg tcggattcgc acctttcaat aatcccagcg ccttgcgcca ctcccagcct
     1081 gaaacatcta actcgtcgtc aataggcaac tcaataacat cgcgcggcgc ctctactcgc
     1141 agataccatt ccagcgggtg aacatacaaa aaccgtacat cgtagtcgct gtcgggcgag
     1201 gcaaatcccc agccgcggct ccctgattca caggcgtaca gtactttcac atcataccgc
     1261 cgttcaattt ctcttaactg ctgcgatacc ctttctcgca tcgcagcact gactccattt
     1321 aatggcatgt tactcaccct ttgatattaa ccaacccatg cagaacctga tccagaccgc
     1381 caaatacaga gatgttatcg atccgttcag ccactctctc cagcgtttcc agttctttta
     1441 agcgtaacgc taccgggttg ttttccatga ctttagcggt attgagtaac gaacgcgtcg
     1501 ctgccgtttc ttcacgtcgg cgaatcacat tggcctgcgc ggatttctcc gcctcaacca
     1561 gctgcgcaag gatatttttc atatcgccag gcaggacgat atctttcacc cctaacgagg
     1621 caatctccat accaaatggc aacatgcgcg atttcacctg ttcgctgaca acgtcatcaa
     1681 tgacctgttt atcttccagc aattcatcca acgtacgcgt acctaccgct tcacgcaggg
     1741 caaattgtaa ttcccgatac aaatgatcaa tcggcttcgt taactggcta aatgccaata
     1801 acacgtcgct gtagcgccag ttagcggcga ggttaatccg cagattgacc ttatctttcg
     1861 ttaagatttc ttgcccgctg acttccagta cctgcaagcg ggtgtcaacc acttcagcgt
     1921 caaccagatg gttaattttc cagtatgcgg tcagccccgg cggtaacagc gcctgcgttt
     1981 caccatcgat tttcagcacg cctacatgcc aggccggaac ctgcaccgtt agtattgcat
     2041 cgcggccttt aactgcccct ttgcgccgtg gttgcaatac cgcattcatc acctccgttt
     2101 gtacctggac ttgctgcgta ttcatccgca ccagtgtcaa atcatcctct acacgccagt
     2161 acaaacggcg cgttgatggc ggcaggatct cctgcaaaat accgtccata tacaacgccc
     2221 cggcttcagt ctccgacaag tcggccacca cgcaatatct ttccacccaa tcaggctgaa
     2281 agcggcgcaa ataatccgcc agcacgtcgg gcacttcgct accatccagc gtaatcagca
     2341 acacttccgg ggtagtaaac cagggtaaaa tatgctcgcc tgcatgtagc actttgtagt
     2401 aatcgccgtt tctggagagt aatgccagct gatcttttct tacgcttatt ttcttaatca
     2461 ttatctattc cttgcttaac agccgtcgcg gaagcggaca cgaagcgaca ggggaattcc
     2521 cttttgcctg ttacccggcg tctttatcgg cgacgttatc ggggatcgct gactgcgcaa
     2581 tccctcattt tttatttaat aagttacaac cagtattcat ttcaggaatg aatgtgggaa
     2641 tcgaacccat aattggcgtc aaaggctgac ggaacacgcc cggaaaacag cctgaccgtt
     2701 cctggtgtgc cgacggggtt taaccccctt tgaaattcag tatcaacgca aaagtgttat
     2761 tgcgagaagc gtgccagaat tgagaaaaat tcttaaccaa atgaaaaata aagagttagt
     2821 gaattacttt tgtttgtagg gggaaaactt ttcgctaaat gtgtatctgt atttatcgtc
     2881 aattatcaag aaagataaat atttaagaag agaacaaagg aatattatca gttctgcttg
     2941 caaatgaatt cccgtacact gaatccatcg gctaactgag ggcaacatca tggcactccc
     3001 gcgtattacc caaaaagaga tgacagagcg cgagcaacgc gagctaaaaa cgctgctgga
     3061 ccacgcgcgg attgcgcatg gtcgcgtact gaccaactcc gaaaccaaca gcattaaaaa
     3121 agagtacatc gataagctga tggttgagcg tgaagcagct gcaaaaaaag cccgccagtt
     3181 gaagaaaaag caggcttata aaccagatac tgaagcatcg tttaactggt cggctaatac
     3241 accaacgcac ggcaggcgct gattaacgcc ccttcttttt acgccccggc gatgtgaaac
     3301 gtttaccatt tgacgccggg cggccacctt tttctgatgt tttttccatc ttcaccaacg
     3361 ggcgtttgat gcccgccgtt ttcggtttgg ccttcacttt aggttttgcc tcggaagagg
     3421 aattttcgat gagcttaaac aggtcgatta actcatcgtc agttaaatcg cgccactcac
     3481 ccagcggaat gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt
     3541 catagccgaa atgctcgcac atgcgccgga tctgacggtt cagcccctgc accagggtaa
     3601 tgcggaagac aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga
     3661 tcggcacccc cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca
     3721 ccagatactc tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt
     3781 gattggtgag gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc
     3841 gtttgctgtg gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga
     3901 caataccgac gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa
     3961 tcaactgacc gtttactttc acaacgtcgc cgggtttcac ctgatcgcca atggtggctc
     4021 gcttgccatt aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc
     4081 aaattccgct ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt
     4141 ctcctgtaaa aaacggaata taccgtacct ttgggttgat aaaaaatagt catgagcgaa
     4201 ctatttgtta tttccattgg cttgaattcg ttacctgctc aaatcacttt ccagaagcaa
     4261 tgtgcgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag catgcctccc
     4321 gtgatttctt actaaccgga gaagtaatgg aactgctttt attgagtaac tcgacgctgc
     4381 cgggtaaagc ctggctggaa catgcactgc cgctaattgc tgaacagttg cagggtcgcc
     4441 gctcagcggt gtttatccct ttcgctggcg taacgcagac ctgggatgat tacacagcga
     4501 aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat agcgttgtcg
     4561 atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt cggcggcggg aatactttcc
     4621 agttgctgaa acagtgccgc gagcgcgggc tgctggcacc aattactgac gtggttaaac
     4681 gtggcgctct gtatattggc tggagcgcag gcgctaacct tgcttgccca accattcgta
     4741 ccaccaacga tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat ctgttcccgt
     4801 tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt gaaacccgtg
     4861 agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact gacgattatt ggtctaccgg
     4921 aaggtaactg gatcacagtg agtaaaggtc acgctacgct gggtggcccg aacaccactt
     4981 atgtgtttaa ggctggtgaa gaagcggtgc cgctagaagc tggtcaccgt ttttaagtga
     5041 attgccggat gcatgatccg gcatcttgtt catagtgcct gatgcgacgc ttgacgcgtc
     5101 ttatcaggcc tacaggattc acaatattaa tactcgtccc gtccttcatc ttcatccggc
     5161 tgttccagca cactgtaagc caccgaacaa aacagcgagt tcaggcgctg catatctccc
     5221 agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg atgcaggcga
     5281 tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa agcgatgctt actacggcgc
     5341 aaacgacgag cgctggtgac atcgccagag aagaacaccg acattgccag ttttaaatta
     5401 ctgagcaatt gctcatagag cgcatccagt tccttcaagc catcaagcga aaatgcccgc
     5461 cgtgctgcca gcgatttatc ggcaatttcg ccgcccatgc gctcgacgat atcggaggcc
     5521 tgttcaaggt tgagcgacat ttcgatgatc tccgcccagc ggcgcgactc ttcctccgcc
     5581 aactcttctt ttggcatccg cgccagatac agcttaatgg cggtgtagag aacgttgata
     5641 tcatctgcca gcttacgcag ctctttctcc tgccgtggct cgccgtgcat cactttattt
     5701 agcccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg cgcggcgttc
     5761 gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca gatgtttggg ccgtagctgg
     5821 gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat cgggtcaaca
     5881 aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata gatcaccagt
     5941 tccgcttttg gcaatgacaa cttccccatt gtctctgcca gcaaatggac aaacggcagg
     6001 ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg gcgggctgcg
     6061 gcattggcgg cactgttgtt gagcatcgcc agcagaccgg aaccgaggtt agcaccaatc
     6121 accagacaga gcgccacggg gaaggagata atgcctgcgg cggtcagagt cgcggtcagc
     6181 agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat cagcgcatcc
     6241 agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc
     6301 ggcgttacgg cctgcacaat caactccagc gccagcaaaa tcagcccaag accaataccg
     6361 acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata
     6421 aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc cattagcgcc
     6481 gtcccgacat ctgcacccag cacaatgacc agagccggtg cgagggctac cagatcctga
     6541 gcgacaaacg aggtcaccag catggtggtg gcattactgc tctgtaccag tgcggtaacg
     6601 ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag gacagtacgc
     6661 aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc ccagaccagc
     6721 agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcaggttc tccttatcgt
     6781 tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac tggcatcttt
     6841 actgtgcctt ttgaccttcg tttttccggc taagattatt ccagcctgat tccagtataa
     6901 gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa
     6961 agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag ccagcgctca
     7021 acttcggtaa cgctcatacc tttacggcgg gcataatctt caacctgatc gcgctgaatt
     7081 tgtgctacag cgtagtactt gctgtccggg tggctgaagt accaacccga aaccgatgca
     7141 ccgggccaca tggcgaaaga ttctgtgagt ttcatgccag tgtgtttttc cacttccagc
     7201 agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccggatagcc cggtgccgga
     7261 cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc gttcggcgca
     7321 tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc ttcggctaaa
     7381 cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca
     7441 tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat gtaatctgct
     7501 ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa tcccgttttt
     7561 tcggtctgtt gacgcagatg gtggctgacg ttgatcacat gggtgcgcgt ttcgtcacgg
     7621 tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac gccacgcgga
     7681 ttcagcgttt tctcggcgct taatttatcc agcatgtcgt tggcgtcttt aaacagccgc
     7741 tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg gatacttccc ggccagcgac
     7801 caggtcataa agaacggtgt ccagtcgatg taattacgca gagtttcgat gctggcttca
     7861 acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca gtcaaaagcg
     7921 aaatcgttat cgcgcgccgc ttccagcgtg accggtggtg tgcgcggttt cttacgcccg
     7981 tgctgaatac gtacggtttc gtactccttg cgggtacgag cgacaaaatc atcacgctga
     8041 gtatcggaaa gcagcgccgc caccacacca acggtgcgcg atgcgttctg tacatacacc
     8101 gtcgggccgc tgtagttctg ctcaattttc accgccgtgt gcgcttttga ggtcgtcgcg
     8161 ccgccaatca atagcgggat ggtgaagccc tgacgctcca tctctttcgc cacgttaacc
     8221 atctcgtcca gcgacggcgt gataagcccc gaaagtccaa tcagatcagc attcacttct
     8281 ttagcggtac ggagaatttt ttccgcaggc accataacgc cgagatcgac aatttcgtag
     8341 ttgttacatt gcagcaccac gccaacgata tttttaccga tgtcgtggac atcgcctttc
     8401 acggtggcga tcaccatctt gccgttggtt ttaccctgct ctttgctggc ttcaataaac
     8461 ggttcgagat acgccactgc ctgtttcatg acgcgcgccg atttgaccac ctgtggcagg
     8521 aacattttcc cttcgccaaa cagatcgccg accacgttca taccgtccat cagcgggcct
     8581 tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcggtatc ctgctcgata
     8641 aactcggtaa tgcctttaac cagcgagtat tccagacgtt tattcacttc ccacgagcgc
     8701 cactccgcct gctgggcgtt agcggtgtcg tcggttttgc tgccgcgata tttctcggca
     8761 agctccagta aacgctcagt gccatcgtcg cgacggttaa gaatcacatc ttccaccgcg
     8821 tcgcgcagtt cagcgggtag gtcgtcgtaa atcgccagtt gcccggcgtt gacgatcccc
     8881 atatccatgc cattgcgaat agcgtagtag aggaacaccg cgtgaatggc ttcgcgcacc
     8941 ggatcgttgc cacggaatga gaaagaaacg ttagatacac cgccggaaat cagcgcgtgc
     9001 ggcagttcgc gtttgatatc ttcacacgcg ccgataaagt cctgcgcgta gttgttgtgc
     9061 tcttcaatgc cggttgcgac cgcgaagatg ttcgggtcga agatgatatc ttctggcggg
     9121 aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcgat tttccgtgcg
     9181 cgggtatcgg cctgtccctg ttcgtcaaag gccattacca ccactgccgc accgtaacga
     9241 cgcaagagct tcgcgtgatg gataaaggcg tcgacgccct ctttcatcga gatagagtta
     9301 acaatgcctt tgccctggat acacttcaga cctttttcaa tgacatccca ttttgaggag
     9361 tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt gagaaaacgc
     9421 accatcgccg cttcggcgtc gagcatccct tcatccatgt tgatatcgat aatctgcgca
     9481 ccgttttcca cctgctggcg ggccacatcc agtgcctcgc tgtatttctc ttctttgatc
     9541 aggcgcttga acttagcgga accggtgacg ttggtgcgtt cacccacgtt cacaaacagg
     9601 ctatcttcgc caatgttcag cggctccagg ccggacaaac ggcaggctac ggggatttcc
     9661 ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgctgcaat atgttgtggc
     9721 gtggtgccac agcagccgcc gacgatattg agaaaacccg cttgcgccca ttcacgtatc
     9781 tgttttgcca tcgtgtcggc gtcgagatcg tactcaccaa aggcgttggg tagcccggcg
     9841 ttcgggtgcg cggtgacgta gcattccgca atccgtgaca gctcctgcac gtactggcgc
     9901 agttcatcgg gccccagcgc acagttcagg ccaaaggtca gagcttcggc gtggcgcaat
     9961 gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg
    10021 gtgccggaga tcataatcgg cagctcaacg cccagcgctt caaactccgt tttcaccgca
    10081 aaaaccgccg ctttggcgtt aagggtgtcg aaaacggttt caatcaggat cagatccgcg
    10141 ccaccttcca ccagcgcttt ggtggactct cgataagccg ccaccagccc gtcaaaagtg
    10201 atattacgaa atgccggatc atttacgtcc ggagaaatag acgccgtgcg gttggtcggg
    10261 ccgagaacac cggcaacgta gcgcggtttc tccggcgtgc gcgcggtcca ctcatcagca
    10321 caagctcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga ttccatctgg
    10381 taatccgcca tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat atccgcgccc
    10441 gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttgctgag caccagcagg
    10501 tcgttgttgc ctttgagatc gcatggccag tcggcaaagc gttcaccacg aaaatcggct
    10561 tcgttcagtc gatagctctg gatcatggtg cccataccgc cgtccagcac cagaatacgt
    10621 tgatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc cgacacgctc
    10681 aacccagatt taataaaaat tcaacaaacc atactggcat aaacgcatct gtggtaaaag
    10741 cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt tcagcaacta
    10801 ttgcattagc taacaataaa aatgaaaatg atttccacga tacagaaaaa ggaggctgtc
    10861 atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg
    10921 actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat tgccgaatcc
    10981 aatggcagtg tggcactcac agagctggcg caacaagccg ggttacccaa ttccacgacc
    11041 caccgcctgc taaccacgat gcaacagcag ggtttcgtgc gtcaggtcgg cgaactggga
    11101 cattgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca gagccgtaat
    11161 ttgttagcga ttgttcaccc tatcctgcgc aatttaatgg aagagtctgg cgaaacggtc
    11221 aatatggcgg tgcttgatca aagcgatcac gaagcgatta ttatcgacca ggtacagtgt
    11281 acgcatctga tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca cgcttccggt
    11341 gcgggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct gctgcaccgc
    11401 aaagggttac atgcctatac ccacgcaacg ctggtgtctc ctgtacattt aaaagaagat
    11461 ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg aggaacatgc actggggcta
    11521 cgttgccttg cagcgtgtat tttcgatgag caccgcgaac cgtttgccgc aatttcaatt
    11581 tccggaccga tttcacgcat taccgatgac agcgtgaccg agtttggcgc gatggtgatt
    11641 aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctgactttt tctggcgggc
    11701 agaggcaata ttctgcccat catacctgag tggcaataga ataagggtgt ctgttaatcg
    11761 cattgacgcc aaaataactt aatgtcatac acttcacaca cttttattaa ctcagcatta
    11821 tttttaaaca tcaaaccact taatcattaa caaaatcgca agatctcatt ccaccgaaac
    11881 ttttacggct gcattatgag attgagcgat ttatgatatt t
//
LOCUS       DAZFWP020000146        11851 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFWP020000146 DAZFWP020000000
VERSION     DAZFWP020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.146"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..558
                     /locus_tag="ACHK0Z_RS19570"
                     /old_locus_tag="pgaptmp_003914"
     CDS             <1..558
                     /locus_tag="ACHK0Z_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503856.1"
                     /translation="KFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIV
                     PDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSG
                     NYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKA
                     WLVGAADAPDLGNFEANTTFQITYL"
     gene            640..3282
                     /gene="yfcU"
                     /locus_tag="ACHK0Z_RS19575"
                     /old_locus_tag="pgaptmp_003915"
     CDS             640..3282
                     /gene="yfcU"
                     /locus_tag="ACHK0Z_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HGU4503857.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVANNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3302..4054
                     /locus_tag="ACHK0Z_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             3302..4054
                     /locus_tag="ACHK0Z_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4503858.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4029..4577
                     /locus_tag="ACHK0Z_RS19585"
                     /old_locus_tag="pgaptmp_003917"
     CDS             4029..4577
                     /locus_tag="ACHK0Z_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503859.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4574..5044
                     /locus_tag="ACHK0Z_RS19590"
                     /old_locus_tag="pgaptmp_003918"
     CDS             4574..5044
                     /locus_tag="ACHK0Z_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503860.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5041..5565
                     /locus_tag="ACHK0Z_RS19595"
                     /old_locus_tag="pgaptmp_003919"
     CDS             5041..5565
                     /locus_tag="ACHK0Z_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503861.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5552..6424
                     /locus_tag="ACHK0Z_RS19600"
                     /old_locus_tag="pgaptmp_003920"
     CDS             5552..6424
                     /locus_tag="ACHK0Z_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU4503862.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6546..7097)
                     /gene="smrB"
                     /locus_tag="ACHK0Z_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             complement(6546..7097)
                     /gene="smrB"
                     /locus_tag="ACHK0Z_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU4503863.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7263..8195
                     /gene="prmB"
                     /locus_tag="ACHK0Z_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             7263..8195
                     /gene="prmB"
                     /locus_tag="ACHK0Z_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4503864.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8230..9315
                     /gene="aroC"
                     /locus_tag="ACHK0Z_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             8230..9315
                     /gene="aroC"
                     /locus_tag="ACHK0Z_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU4503865.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9319..10143
                     /gene="mepA"
                     /locus_tag="ACHK0Z_RS19620"
                     /old_locus_tag="pgaptmp_003924"
     CDS             9319..10143
                     /gene="mepA"
                     /locus_tag="ACHK0Z_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU4503866.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10143..10952
                     /locus_tag="ACHK0Z_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             10143..10952
                     /locus_tag="ACHK0Z_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU4503867.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10952..11500
                     /gene="epmC"
                     /locus_tag="ACHK0Z_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     CDS             10952..11500
                     /gene="epmC"
                     /locus_tag="ACHK0Z_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU4503868.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11534..11812
                     /locus_tag="ACHK0Z_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             11534..11812
                     /locus_tag="ACHK0Z_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU4503869.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2933 a         2890 c         3095 g         2933 t
ORIGIN      
        1 aagtttgtta aaacagctat tgctgcggca atggtgatgg gcgttttcac ctctacggca
       61 acaatcgctg cgggtaacaa cggtactgca cgtttttacg gcaccattga agactcagtg
      121 tgttctatcg ttccggacga tcacaaactg gaagttgata tgggtgatat cggtgccgaa
      181 aaactgaaaa ataacggcac caccacgccg aaaagtttcc agattcgtct gcaagattgc
      241 gtatttgata ctcaggaaac aatgaccact acctttactg gtaccgtttc ttctgcaaat
      301 agcggcaatt attacaccat tttcaatacc gataccggtg cggcatttaa caatgttagc
      361 ctggcgatcg gtgactctct gggtacctct tacaaaagcg gcatgggtat tgaccagaaa
      421 atagtgaaag acacttctac caataaaggc aaagcgaagc agacactgaa ctttaaagcc
      481 tggctggtcg gcgcagctga tgcgccagat ctgggtaatt tcgaagcaaa taccaccttc
      541 cagattactt acctgtaagg cataccccca tagcgacact gctatggggg atttaaatgg
      601 attttgaccg aacaagaata aaaacggata gaaacgtgta tgcctgacca ttctcttttt
      661 cgattacgga ttcttccgtg gtgcattgcg ctggcaatgt cagggagtta tagcagtgtc
      721 tgggctgaag acgacattca gtttgattcc cgttttctgg aattaaaagg cgacacaaaa
      781 attgatctga agcgtttttc cagccaggga tatgttgagc ccggaaaata caatttacag
      841 gttcaactaa ataaacagcc attggcggaa gagtacgata tttactggta tgctggtgaa
      901 gatgacgcga gcaaaagcta tgcttgtctg acaccggaac tggtagcgca gtttggttta
      961 aaagaagacg tggcgaacaa tctgcaatgg agccacgatg ctaaatgcct gaaatccggt
     1021 caactggaag gcatggaaat taaggctgat ttaagccagt ccgcattagt catttcactg
     1081 ccacaggctt acctcgaata tacttatccc gactgggatc cgccttcacg ttgggatgac
     1141 ggcatctccg ggatcgtcgc ggactacagc atcaacgcac aaacccggca cgaagaaaat
     1201 ggcggtgatg atagtaacga gatcagcggc aacgggacgg tcggggttaa cctggggccg
     1261 tggcgtatgc gtgctgactg gcagactaac tatcaacata ctcgcagtaa tgatgacgat
     1321 gaattcagcg gcgatgaaac tcaaaaaaaa tgggagtgga gtcgctacta tgcctggcgg
     1381 gcgttaccat cattaaaagc caaactggcg ctgggcgagg attacctcag atccgatatt
     1441 tttgatggtt ttaactatgt tggtggcagt gtcagtactg acgatcaaat gttgcctccc
     1501 aatctgcgcg gctacgcgcc agacatttcc ggcgtggcac acaccacagc aaaagtgacc
     1561 gtcagccaga tggggcgtgt gatttacgaa acgcaggttc cggctggacc gtttcgtatt
     1621 caggatcttg gtgattctgt ctccggtacg ttgcatattc gcattgaaga acagaacggc
     1681 caggtgcagg aatatgacat cagcaccgcc tcgatgccat acctcactcg cccaggtcag
     1741 gttcgctata agatcatgat gggccgtccg caagagtggg gacaccatgt cgagggtgaa
     1801 tttttttctg gtgctgaagc ttcctggggg atcgctaacg gctggtcgtt atatggcggc
     1861 gcactgggag atgaaaacta tcagtctgcg gcgcttggcg tcggtcgcga tttgtctaca
     1921 ttcggcgcgg tcgcgtttga tgttactcac tcgcacacca aactggataa agacaccgct
     1981 tatggcaaag gttcgctgga cggtaactcc ttccgtgtga gttattccaa agactttgac
     2041 cagctcaaca gccgcgttac ttttgctgga tatcgcttct cggaagagaa ctttatgacc
     2101 atgagcgagt atctggatgc cagtgacagc ggaatggtac gcacgggcaa cgacaaagag
     2161 atgtacaccg ccacttataa ccagaacttc cgcgatgcgg gtgtttcggt ttatctcaac
     2221 tatacccgcc atacctactg ggatcgcgag gagcagacaa actacaacat catgctctcg
     2281 cactatttca atatgggcag tattcgtaat gtcagcatct cgatgactgg ctaccgttac
     2341 gagtatgaca accaggccga caaaggcatg tacatttcgc tcagtatgcc gtggggcgac
     2401 aacagtaccg ttagctataa cggtaactat ggcagtggga cggacagcag tcaggtcggt
     2461 tatttcagcc gtgtcgatga cgcgactcac tatcagttga acgtcggcac cagtgacaaa
     2521 cacaccagcg ttgacggcta ttacagccat gatggttcgc tggcgcaggt tgacctcagt
     2581 gcgaactacc atgaagggca atacacctct gcgggcttgt cgttacaggg cggcgcaacg
     2641 cttactgccc acggtggcgc acttcaccgt acccagaata tgggcgggac acgcttgttg
     2701 attgatgccg atggcgttgc cgatgttccg gtagaaggta acggtgctgc tgtttatacc
     2761 aatatgtttg gtaaagccgt cgtttctgac gtcaataact attaccgcaa tcaggcgtat
     2821 atcgacctca acaaactgcc ggaaaacgcc gaagcaaccc agtcggtggt acaagccacg
     2881 ctaactgaag gggcgattgg ctaccgcaaa tttgccgtca tcagtggtca aaaagcgatg
     2941 gcggtgctgc gtttacaaga cggcagccat cctccgtttg gcgcagaagt aaaaaatgat
     3001 aacgagcaga cagtgggcct tgtcgatgat gacggcaatg tttatctggc tggggtgaaa
     3061 cctggcgaac acatgagtgt gttctggagt ggtgttgcgc attgcgatat caacctgccg
     3121 gacccgctgc ctgccgatct gtttaacggc ttgttactgc catgccagca taaaggcaac
     3181 gtcgcgccgg ttgttcctga tgacataaag cctgtcattc aggagcagac gcaacaggtg
     3241 acacccacca atccccctgt ttctgtttca gctaaccaat aacgtgatta aggaatgatc
     3301 catgtctgat ttactttgct ctgccaaatt gggtgctacg acgctagcgt tactgcttag
     3361 cgctgccagt ttgtctgccc aggcttctgt aacgccagat cgtacccgtt tgatatttaa
     3421 tgaaagcgac aaatcaatca gcgtgacgtt gcgcaataac gatccgaaac tgccttatct
     3481 ggcccaaagc tggatcgaag atgaaaaggg taacaagatc agttcaccgt taactgttct
     3541 gccaccggtg caacgcatcg attcaatgat gaacgggcag gtaaaagtgc agggaatgcc
     3601 ggatatcaac aaattgcctg ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat
     3661 cccgccaaaa tcaaacaaag ccaacacgct gcaaatagca ctacaaacgc gtattaagtt
     3721 gttctggcga ccgaaggcgc tggagaacgt cagcatgaaa aacccctggc aatataaggt
     3781 tactttaacc cgcaacggtc aggaatttac cgtcaataac ccgacaccgt attacgtcat
     3841 tttcagtaat gccagcacac agaaaaatgg caatccggca gccggtttct cgccaatagt
     3901 gatgtcgccg aaaacctctg caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac
     3961 ctatgtcaac gactatggcg ctcggatgcc gctctttttc acttgcaacg gtaacagttg
     4021 ccaggtcgat gaagagcaga gccgcaaagg ctgaggccgg gagagccagt atgaaaggat
     4081 cttgttctgt ctgggcggta actgggttac tgatatgttg tgttggtagc ggctttgcag
     4141 ttgcagcgag taagtcagta agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt
     4201 gtacggtaaa tggtgcggca gttgagtttg gtaatgtatt cattaataag atcaatgggg
     4261 ttgattataa acgtcccatt gactactcgc ttgtttgtaa caatctggca atggatgacc
     4321 tgcgtctaca gatgcaggcg acaacggtcg tgattaacgg tgaaacagtt atcagcacgg
     4381 ggattcctgg gtttggtatt cgggttcaga aaagtagtga tcacacaata ctcgacttaa
     4441 cctctggctc atggttgcca tttaattttt cctcgggagt tcccgttctg gaagctgtgc
     4501 cggtgaaaca gagtggtaca acgttagcgg cagctgaatt taacgccagt gccacaattg
     4561 tggtggatta ccaatgaaac ggatctcgct tattttgctg tggggctttt gctcgatggc
     4621 actctcaaac gtctcttttc acggttattt agtgcagcca cctaactgca cgattagcaa
     4681 cgctcaaacc attgaaataa cttttcagga tgtgcttatt gatgacatta atggcagcaa
     4741 ttatgagcaa accgtgccgt attccattac ctgcgatacg gcggttcgcg acccattaat
     4801 ggaaatgacc ttgagctgga gtggcacacc aagcgatttt gataatgctg cagtaagcag
     4861 caatatcact ggtttaggca ttcagctcaa acaggcaggg cagagtttta cgatcaatac
     4921 gccacttgtg gtgaatgaaa ccgatttacc ggtgttaacg gctgtgccag taaaaaagag
     4981 tggtgtcgtt ctgccagagg cggactttga ggcctgggcc acgttacagg tagattacca
     5041 atgaagaaaa agcgtacgtt gttttttatt agtagcctga tgttgctggg cagtggaacc
     5101 acaatagcag gagataattt gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca
     5161 gtgattaatg gtagccagtt ggcagaagta cattttcctg ccattgccgc cagcgattta
     5221 atgaaccttg ggcagtcaga gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt
     5281 tctacattgt ttaatgtcaa agtaacgttg acaggaacag aagatagtgc tctacccggc
     5341 tttctggcct ttgactcctc gtcatcagca tccggcgctg ggattggtat tgaaactgcg
     5401 gcaggcacat cggtgccaat caacaataca acaggcgtca cgctcccgct gaatcaggga
     5461 aataacagcc taaattttaa tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc
     5521 ggagacttta gtgccacagt gacggcaacc tttgagtatt tctgaaatga aaaaatggac
     5581 cataattttc gcgagcctaa tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta
     5641 tgctggagat aaacttatga gtgcgagttt tgacagtgtg agagtttatt atgccatgga
     5701 caaggtaacc ggtgctgtag cgagcagtgt tttcaatgtt acggtgataa cgccaaagga
     5761 ggttgcatat gggaaatatg attcttttgc gtataaaggc gatacgttgc gggttatttc
     5821 ctggtctggg tcgggttcag ctccaacttt ggtactgact gactttgata caattaataa
     5881 ctcaaattgc cctggcatag atacgaaaat ttttcgttgt gcatatatga cgtttaaaat
     5941 taccgttgca tctgatgatt atggttgccc ttggatcgca tccttttatt cctatacaga
     6001 tttacccgga tttggaagct atacggcccc tacagtgcat aacacaatat gcccaacgat
     6061 accggtcgct tcatacgata tctcctggag tgaaaattat gtcagccata ataaggcgtt
     6121 acgaattcag tccacggggt caacagtgac caccacgctt tcgacctact tgatggaagg
     6181 cggcagactc tgtgatggca gtaatttttc tgataatgat ggcagaggag catactgccg
     6241 cgcggtatca gaattgttaa cctttacctc ttacggttgt gataaatcaa cggtaacggt
     6301 tacgccaact cgtcatccgg taacggataa agtactacac gatattgtgg tgaatgtgaa
     6361 taccagtagt gggcaaccga ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact
     6421 gtagttcgta gtattgctct gatgcgtctt atcaggccta cgaagtgaat tgcaatttat
     6481 tgaattatca catatttgta ggccggataa ggcgtttacg ctgctccggc atttgctgcc
     6541 agcattcagg gcaactccgg cggcaaccac tcttccactt caatcaacac caacaaagcc
     6601 gcatcaccgc cgtactcttt cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc
     6661 agccacagcg gtgtttgttg cttcaaaata tgcttcccgt gcccatgcat cacgcaggca
     6721 caaaacacat gttcgcggcg acaggcggca atcaacgcgc ccagttcctg cttggcctgc
     6781 agctgcgtca gaccgtgtaa atccaaaaac aattccggtg aataatcgcc gcggcgcagt
     6841 ttcttcgcct caaaatggct gacatccggg cgaacatatt tcaccggacc ttcggtattt
     6901 aataacggct gaaactcatc ggagaaataa tggctggcat cagcctgctc ctggatcaag
     6961 cgtttcaccg gcacttcgct gattttttta cgctgcggtc ggtggacaat cgtgtcctgc
     7021 ttaatcttgc gagtccccgc catcaactgg cgaaacagag cctggtcctc ctcgctaagt
     7081 gttgttttct ttttcattta tctgtctcta tttctaattt tcctacagtt tacccgactc
     7141 gtagcacaat cgatcgtttt aaacgtattt ttcctcactc accgcgcgtg aatgctgatt
     7201 tatcgtcatc ttcatggcaa actagccgcc gaaattattg cgatcatgcc ctggaggaat
     7261 acgtggataa aattttcgtt gatgaagcag taaatgagct gcaaaccatt caggacatgt
     7321 tgcgctggtc ggtaagccgc ttcagcgcgg caaatatctg gtacggtcac ggcaccgata
     7381 acccgtggga tgaagccgta cagctggtgt taccatcgct ctacctgccg ctggatatcc
     7441 cggaagatat gcgcaccgcg cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg
     7501 tgatccgccg cgtcaacgag cgcattccgg tggcttacct gaccaacaaa gcctggttct
     7561 gcggtcatga attttacgtc gatgaacgcg tgctggtgcc gcgctcaccg attggtgaac
     7621 tgatcaacaa taaatttgcc ggactcatca gcaagcaacc gcagcatatt ttagatatgt
     7681 gtaccggtag cggctgcatc gccattgcct gtgcttatgc cttcccggaa gcggaagtcg
     7741 acgcggtaga tatctctccc gacgcgctgg cggttgccga acagaacatc gaagaacacg
     7801 gtctgatcca caacgtcatt ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc
     7861 aatacgatct gattgtcact aacccgccgt atgtcgacgc ggaagatatg tccgacctgc
     7921 caaacgaata ccgtcacgag ccggaactgg gcctggcatc tggcaccgac ggcctgaaac
     7981 tgacgcgtcg cattctcggt aacgcggcag attaccttgc ggatgatggc gtgttgattt
     8041 gtgaagtggg caacagcatg gtacatctta tggaacaata tcccgatgtt ccgttcacct
     8101 ggctggagtt tgataacggc ggcgatggag tgtttatgct caccaaagag cagcttattg
     8161 ccgcacgaga acatttcgcg atttataaag attaagtaaa cacgcaaaca caacaataac
     8221 ggagccgtga tggctggaaa cacaattgga caactctttc gcgtaaccac cttcggcgaa
     8281 tcgcacgggc tggcgctcgg ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg
     8341 gaagcggacc tgcaacatga ccttgatcgt cgtcgccctg ggacatcgcg ctataccacc
     8401 cagcgccgcg agccggatca ggtcaaaata ctctccggtg tttttgaagg cgttactact
     8461 ggcaccagca ttggcttgtt gatcgaaaat accgaccagc gttctcagga ttacagcgca
     8521 attaaagacg tcttccgtcc aggccatgct gattacacct acgaacaaaa atacggtctg
     8581 cgcgattatc gcggcggtgg acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca
     8641 ggggcgattg ccaaaaaata tctcgccgag aaatttggta ttgaaatccg cggctgcctg
     8701 acccagatgg gcgacattcc gctggaaatc aaagactggt cgctggttga acaaaatccg
     8761 ttcttctgcc cggaccccga caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa
     8821 aaagagggcg actccatcgg cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga
     8881 cttggcgagc cggtctttga ccgcctggat gccgacatcg cccatgcgct gatgagcatc
     8941 aacgcggtga aaggcgtgga aattggcgac ggttttgacg tagtggcgct gcgcggcagc
     9001 cagaaccgcg acgaaatcac caaagacggt ttccagagca accatgcggg cggcattctc
     9061 ggcggtatca gcagcgggca gcaaatcatt gcccatatgg cgctgaaacc gacctccagc
     9121 attaccgtgc cggggcgcac tattaaccgc tttggcgaag aagttgagat gatcaccaaa
     9181 ggccgtcacg atccctgtgt cgggatccgt gcagtgccga tcgcagaagc gatgctggcg
     9241 atcgttttaa tggatcacct gttacggcag cgggcgcaaa atgccgatgt gaagactgat
     9301 attccacgct ggtaaaaaat gaacaaaacc gcgattgcgc tgctggctct gcttgccagt
     9361 agcgctagcc tggcagcgac gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca
     9421 caatcgatag gcagtttttc taatggctgt attgtcggcg ctgacacgtt gccgatacag
     9481 tccgaacatt atcaggtcat gcgtaccgat cagcgtcgct atttcggtca cccggatctg
     9541 gtgatgttta tccagcgtct gagtagccag gtgagcaatc tgggcatggg tacggtgctg
     9601 attggcgata tggggatgcc cgctggtggg cgtttcaacg gcggtcatgc cagccaccag
     9661 accggactgg atgtcgatat ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag
     9721 ctcttgcgcc cgcaagcact ggacttagtt tcccgcgacg gtaaacacgt tgttccgacg
     9781 ctgtggaagc cagaaatttt cagcttgatc aaactcgccg cccaggacaa agacgtcaca
     9841 cgcatttttg ttaatccggc gattaaacaa caactttgcc ttgatgcggg caccgatcgc
     9901 gactggttgc gcaaagtgcg accctggttc cagcatcgcg cgcatatgca tgtacgatta
     9961 cgttgtcctg ccgatagtct ggagtgtgaa gatcaacctt taccgccacc aggcgatggt
    10021 tgcggggcag aactgcaaag ctggtttgca cctccaaaac cgggaacaac aaagcctgag
    10081 aagaagacac cgcctccgtt gccgccttcc tgccaggcgc tactggatga gcacgtgatc
    10141 taatggaaac gtttaatagc ctgtttatgg tttccccgct gttgctggga gttctctttt
    10201 ttgtcgccat gctggcggga tttatcgact cgattgccgg tggcggtggg ttactcacca
    10261 ttccggcatt gatggcagcg ggaatgtctc ccgctaacgc gttggcaacc aataaactgc
    10321 aagcctgcgg cggctctatt tccgccacta tctactttat tcgccgcaaa gtggttagtt
    10381 taagcgatca gaaactcaat attgccatga cctttgtcgg ctcaatgagc ggcgcactgt
    10441 tggtacagta cgttcaggct gatgtcttac ggcagatttt gcccattctg gtgatttgta
    10501 tcggcctcta tttcttgctg atgccaaagc tgggtgaaga agatcgccag cgccggatgt
    10561 acggtttacc cttcgcgtta attgccggtg gctgcgtcgg tttttatgac ggattctttg
    10621 gcccggctgc cggatcgttt tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg
    10681 ccaaagccac ggctcacgcc aaattactca acgcaacgtc aaacattggc ggtctgctgc
    10741 tgtttattct cggcggcaaa gtgatttggg cgacgggctt tgtgatgctg gtggggcagt
    10801 tcctcggcgc gcgcatgggt tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc
    10861 cgatgattgt tatcgtctcg gcggtgatga gtgccaaatt actttacgat agccacggac
    10921 aggagatcct ccactggttg gggatgaact aatgaacagt acacaccact atgagcagtt
    10981 gattgaaatt tttaatagct gctttgccga tgaatttaat acccgtctga ttaaaggcga
    11041 cgacgaaccg atctatcttc ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc
    11101 ccacggcttt tatgccagcg caattcatga gatttcgcac tggtgtattg ccggaaaagc
    11161 gcgccgtgag ctggttgatt tcggctactg gtattgcccg gatgggcgtg atgcccaaac
    11221 gcaaagccag tttgaagatg ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt
    11281 ggcggcggga tatccgttta acgtcagttg cgacaatctg gaaggcgact tcgaaccgga
    11341 tcgtgtggtg tttcaacggc gggtacatgc gcaggtgatg gattacctag caaacggcat
    11401 tcctgaacgc ccggcacgct tcattaaagc actacaaaat tattatcaca cgccggaact
    11461 tacggcggaa cagttcccgt ggccggaagc gctcaactaa agccagcgcc acgttttaca
    11521 atgaggaaag aagatgatcg cggagtttga atcacgcatt ctggcattaa tcgacggtat
    11581 ggttgaccat gccagtgatg atgagttgtt tgccagtggg tatttgcgtg gccacctgac
    11641 attagccatc gcagaactgg aaagtggtga tgaccactcc gctcaggcgg tgcatacgac
    11701 cgttagccag agtctggaaa aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc
    11761 gctggtgacc gatatgtggg aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc
    11821 gtctggagca ccatgcctga tgcgacgctt a
//
LOCUS       DAZFWP020000147        11634 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFWP020000147 DAZFWP020000000
VERSION     DAZFWP020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.147"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            46..915
                     /locus_tag="ACHK0Z_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             46..915
                     /locus_tag="ACHK0Z_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4503870.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1118..1471)
                     /locus_tag="ACHK0Z_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             complement(1118..1471)
                     /locus_tag="ACHK0Z_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU4503871.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1609..3255)
                     /gene="groL"
                     /locus_tag="ACHK0Z_RS19650"
                     /old_locus_tag="pgaptmp_003930"
     CDS             complement(1609..3255)
                     /gene="groL"
                     /locus_tag="ACHK0Z_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU4503872.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3299..3592)
                     /locus_tag="ACHK0Z_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             complement(3299..3592)
                     /locus_tag="ACHK0Z_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU4503873.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3868..5124
                     /gene="yjeH"
                     /locus_tag="ACHK0Z_RS19660"
                     /old_locus_tag="pgaptmp_003932"
     CDS             3868..5124
                     /gene="yjeH"
                     /locus_tag="ACHK0Z_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGU4503874.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(5140..5616)
                     /locus_tag="ACHK0Z_RS19665"
                     /old_locus_tag="pgaptmp_003933"
     CDS             complement(5140..5616)
                     /locus_tag="ACHK0Z_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU4503875.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5953..7389
                     /gene="aspA"
                     /locus_tag="ACHK0Z_RS19670"
                     /old_locus_tag="pgaptmp_003934"
     CDS             5953..7389
                     /gene="aspA"
                     /locus_tag="ACHK0Z_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU4503876.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7507..8808
                     /gene="dcuA"
                     /locus_tag="ACHK0Z_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             7507..8808
                     /gene="dcuA"
                     /locus_tag="ACHK0Z_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU4503877.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8924..9262
                     /gene="cutA"
                     /locus_tag="ACHK0Z_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             8924..9262
                     /gene="cutA"
                     /locus_tag="ACHK0Z_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU4503878.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9238..10935
                     /gene="dsbD"
                     /locus_tag="ACHK0Z_RS19685"
                     /old_locus_tag="pgaptmp_003937"
     CDS             9238..10935
                     /gene="dsbD"
                     /locus_tag="ACHK0Z_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU4503879.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10972..11547
                     /locus_tag="ACHK0Z_RS19690"
                     /old_locus_tag="pgaptmp_003938"
     CDS             10972..11547
                     /locus_tag="ACHK0Z_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4503880.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2789 a         3010 c         2881 g         2954 t
ORIGIN      
        1 attaaaatta attatttaat tgagaaatgg ttagggagaa cctacatggc cataagcatc
       61 aagggtgtta ataccggtgt tattcgcaag agtaataatt tcattgcact ggcgctgaaa
      121 attaaagaac ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga actgcgcgac
      181 ctgctgattg cgctggaaag tcgtatgcat caaaagcata aactggatgc tgctgcacgc
      241 cttcagtatg agcaagctcg cgataaagtc attaaaaaaa tggcagaaaa catcccagaa
      301 attctggttg atgagcttaa aaatgccgat attaatcgtc gggttaacac gctggaactt
      361 accgataatc aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg tagcaaatgt
      421 gagttagtcg tcaatgagtt gcagattgaa atgctggcgc gagcaatcat tcatgcaatt
      481 aataatgcgg aaatgcgtga actggcatta cgtattacct cactgttaga tttcctgccg
      541 ttatatgacg ttgactgcca ggaaaatggc aatctggaat atgacaccta ttcccaaccg
      601 gaatggaaac ataacctgtt cgaccattat ctggcggtgc tttaccgctt taaagatgaa
      661 agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa
      721 gaaatagagg cgatcactcg ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg
      781 ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga ataacgccca accgctaacc
      841 caggatcaat gcttacgcgc gctgcatcac ttacgtgttc aatccaccag caaaactgca
      901 ccacaggcga aataatctca tctttgctaa tgcgaggctg gagccatcca gcctgaaatg
      961 actgataacg tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc cggcctacaa
     1021 attctcaagc attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca
     1081 ggcaatgata cgtttgtcac ccagcctctg gatcacatca gttcgggcac ttataaacct
     1141 gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc
     1201 tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg
     1261 cgccgcgcat agaaccgccc tcttctccgt gttgcccgga aagccagttg ctttgcttac
     1321 ctgtcgcagt accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac
     1381 tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac
     1441 cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg
     1501 ccactcgcgt cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc
     1561 agacatttct gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc
     1621 gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcattttt
     1681 cggcaggtcg gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta
     1741 ctgcagagca gaacgggtta ctttggttgg gtccaggata cccatgtcga tcatgttgcc
     1801 gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc
     1861 aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc
     1921 acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag
     1981 tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc
     2041 agcacgggtc gcgtgcaggg cgtcttcaac gcgtgctttt ttctctttca tttcaacttc
     2101 ggtagcagca cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgttcctg
     2161 cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac
     2221 acggccctgg attgcagctt cttcacccac gccatcgatg atagtggtgg tgtctttgtt
     2281 gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc
     2341 gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt
     2401 acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt
     2461 gttaacaacc agagttgcca gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg
     2521 tttgcctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt tagagatttt
     2581 cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt
     2641 gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc
     2701 gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat
     2761 cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg
     2821 agcaatcgct ttagagtcag agcacggtac ggacagcgct ttcagttctt caactgcagc
     2881 ggtaacggct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc
     2941 tttcagaccc tcagtgatga tagcctgagc cagtacagtt gcagtggtgg taccgtcgcc
     3001 tgcagcgtcg ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tattttcgaa
     3061 cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc
     3121 accgaaagat ttatccagaa ctacgttacg gcctttcgga ccgagggtaa ctttcactgc
     3181 atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac
     3241 gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt
     3301 acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga
     3361 tcttctcaga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat
     3421 ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc
     3481 cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt
     3541 caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact
     3601 ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc
     3661 tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat
     3721 tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct
     3781 tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa
     3841 aatttgggcg caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc
     3901 caaggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg
     3961 ttagctgcgc tggtagcggg caataacagc ctgtgggcgt ggcccgtttt gattatctta
     4021 gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggt
     4081 gtcgcgcact tcgtcggtat ggcgtttggt tcgcgacttg agcgagtcac cggctggttg
     4141 tttttatcgg tcattcccgt gggtttgcct gccgcgctac aaattgccgc cgggttcggc
     4201 caggcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg
     4261 ctggtgtggt atatcggtac tcgcggtgcc agttccagtg ctaatctaca aaccgttatt
     4321 gccggactta tcgtcgcgct gattgtcgct atctggtggg cgggcgatat caaacctgcg
     4381 aatatcccct tcccggcacc tggtaatatc gaacttaccg ggttatttgc tgcgttatca
     4441 gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa
     4501 aatccagagc gtgattttcc tcgcgcattg atgattggtc tgctgctggc aggattagtc
     4561 tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaaa aatggcggcg
     4621 gcagcatcgc ttccaaaaat tgtagtgcag ttgttcggtg taggagcgtt atggattgcc
     4681 tgcgtgattg gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc
     4741 cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct
     4801 cgccatatcc cgaataatgc cctcaatgcg gtgctcggct gctgtgtggt gagcacgttg
     4861 gtgattcatg ctttagagat caatctggac gctcttatta tttatgccaa tggcatcttt
     4921 attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga
     4981 ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa
     5041 agtctctatg cgctgatcat gctggcgggg ttatggctgt tgctgccaaa acgaaaaacg
     5101 ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt
     5161 atgatcaagg cggtcgcgct catcatcctt tcgctggtac tcaccatcaa aagtattacc
     5221 gccaccggtc ccggcgctaa aaccgccgcc tggcatacga gaaaagcgca aatgcggcat
     5281 caacttcact gtcagatgct tttgcaccgg cggtaataaa agcagaagac cgaggaagtc
     5341 ggtgaaaaag cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat
     5401 catctccgcc gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt
     5461 aaagccctgg ttacgtacca gtgacatccc gataactgac gtgaatataa ccagcacgag
     5521 ggtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct caatatagac
     5581 ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg
     5641 gtaaaacccg gctacctgaa tgggttgcga atcgcgttca gcttatattg tggtcattag
     5701 caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt
     5761 tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga
     5821 aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg
     5881 aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa
     5941 aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa
     6001 gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc
     6061 agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa
     6121 gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc
     6181 attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac
     6241 gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat
     6301 atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac
     6361 catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt
     6421 tactcttccc tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt
     6481 aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta
     6541 ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa
     6601 aatatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt
     6661 actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc
     6721 actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct
     6781 tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac
     6841 gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa cctgccggaa
     6901 ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt
     6961 aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca
     7021 ggtcagctgc agttgaacgt tatggagccg gtcattggcc aggccatgtt cgaatccgtt
     7081 cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct
     7141 aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac
     7201 ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag
     7261 agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt
     7321 ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc
     7381 gaacagtaat cgtacagggt agtacaaata aagaaggcac gtcagatgac gtgccttttt
     7441 tcttgtgagc agtaacttaa aaataacaac ctaatatcaa cttgttaaaa aacaaggaag
     7501 gctaatatgc tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga
     7561 ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt
     7621 ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc
     7681 gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag
     7741 ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg
     7801 actatctttg ctggtactgg caacatatct ctggcgacac tgccagttat cgctgaagtt
     7861 gcgaaggaac aaggcgtcaa accttgccgt ccgctgtcta ctgcagtggt atccgcgcag
     7921 attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa
     7981 ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg
     8041 gttctggtga tgtcctttct ggtcactatg ctattcaact ccaaactctc tgacgatccg
     8101 atttatcgca agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa
     8161 atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt
     8221 atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc
     8281 accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa
     8341 gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt
     8401 tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc
     8461 aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc
     8521 tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggcc
     8581 ctggcactga acgtttcacc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg
     8641 ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtaca
     8701 acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt
     8761 gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg accaatcgcg
     8821 gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt ccgttgtata
     8881 gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag
     8941 ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga cagcccagga
     9001 tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc
     9061 tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt
     9121 aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata
     9181 tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg
     9241 gctcaacgca tctttacgct gatcctgcta ctttgcagca cttccgtttt tgccggatta
     9301 ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttac ttttgatttt
     9361 cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac
     9421 cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgtgca gctgccacaa
     9481 ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg
     9541 cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc
     9601 tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg
     9661 gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg
     9721 caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca
     9781 tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc
     9841 tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac
     9901 acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac
     9961 ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc
    10021 ttgtttactc tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc
    10081 caacagggcg gctcacctgg cggtgtgttt gttatggggg cgattgccgg actgatctgt
    10141 tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac
    10201 atgtggctgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg
    10261 ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc
    10321 aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt
    10381 ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc
    10441 tttatcacca gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg
    10501 gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc
    10561 gcgcaaactc agacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag
    10621 gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc
    10681 gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca
    10741 gacacggtct tacttcaggc caacgtcacg gccaacgacg cacaagatgt ggcgctgtta
    10801 aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag
    10861 catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc
    10921 gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt
    10981 gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg
    11041 ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg cttctggcca
    11101 gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg
    11161 cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagttgct ggcacgttat
    11221 caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt tatcgctgcc
    11281 tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc
    11341 gcggcctacg atttcaccca cgaactgtta accacgctgg aagttgacga tccggcgatg
    11401 gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt
    11461 agccaggcgg atgtcgacac tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc
    11521 tgccgccagg gtggtgcatt gacctgacag aaacacagaa aagaagcgat ttgccgcaat
    11581 cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag atta
//
LOCUS       DAZFWP020000148        11600 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFWP020000148 DAZFWP020000000
VERSION     DAZFWP020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.148"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..6936
                     /gene="yeeJ"
                     /locus_tag="ACHK0Z_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /pseudo
     CDS             1..6936
                     /gene="yeeJ"
                     /locus_tag="ACHK0Z_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(7198..8250)
                     /locus_tag="ACHK0Z_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             complement(7198..8250)
                     /locus_tag="ACHK0Z_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU4503881.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQKYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            8565..9881
                     /gene="shiA"
                     /locus_tag="ACHK0Z_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     CDS             8565..9881
                     /gene="shiA"
                     /locus_tag="ACHK0Z_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU4503882.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            9983..11437
                     /gene="amn"
                     /locus_tag="ACHK0Z_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             9983..11437
                     /gene="amn"
                     /locus_tag="ACHK0Z_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU4503883.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         3114 a         2834 c         2766 g         2886 t
ORIGIN      
        1 tggtaaacgc cgcaacccaa caaccagttc ctgcacaact cgccattgca aatgccaata
       61 cggtgcccta cacccttgga gcgctggaaa tcggcccaaa gcgttgccga acgtttcggt
      121 atttcggtgg ctgagttacg caaactcaac cagtttcgta cgtttgctcg aggttttgat
      181 aatgtccgcc agggtgatga actggatgtc ccggcacaag ttagtgaaaa taatttaacc
      241 ccgccaccgg gtaatagcag cggcaacctt gagcaacaga tagccagtac ttcacagcaa
      301 atcgggtctc tgctcgccga ggatatgaac agcgagcaag cggcaaatat ggcgcgtgga
      361 tgggcctctt ctcaggcttc aggcgcaatg acagactggt taagccgctt cggtaccgca
      421 agaatcacgc tgggcgtgga tgaagatttt agcctgaaga actcccagtt cgattttctc
      481 catccgtggt atgaaacgcc tgataatctc tttttcagtc agcatactct ccatcgtact
      541 gacgagcgta cacagattaa caacggctta ggttggcgtc atttcactcc cacatggatg
      601 tcgggcatca acttcttttt cgaccacgat cttagccgtt accactcccg cgccggcatt
      661 ggcgcggagt actggcgcga ctatctaaaa ttaagcagta acggctattt gcgactgacc
      721 aactggcgca gcgcacctga actggacaac gattatgaag cacgcccggc caatggctgg
      781 gatgtacgcg cagaaggctg gctacccgcc tggccgtacc ttggcggtaa actggtctat
      841 gaacagtatt atggcgatga agtggccctg ttcgataaag acgatcggca aagtaatcct
      901 catgccataa ccgctggact taactatacc cccttcccgc taatgacctt cagcgcggag
      961 caacgccagg gtaaacaggg cgaaaatgac acccgttttg ccgtcgattt tacctggcaa
     1021 cctggcagcg caatgcagaa acagcttgac ccgaatgaag tcgctgcacg gcgtagcctt
     1081 gcaggcagcc gttatgatct ggtggatcgc aacaacaaca tcgttctgga atatcgcaaa
     1141 aaagaactgg tccgcctgac cctgacagac cccgtgacag ggaagtcagg agaagtgaaa
     1201 tcactggttt cgtcgctaca aaccaaatat gccctgaaag gctataacgt cgaagccacc
     1261 gcactggaag ctgccggtgg caaagtggtc acaacgggta aagatattct ggttaccctg
     1321 ccgggttacc ggttcaccag tacgccagaa accgataaca cctggccgat tgaagtcacc
     1381 gctgaagatg tcaaaggcaa tttgtcgaat cgtgaacaaa gcatggtggt cgttcaggca
     1441 cctacgctaa gccagaaaga ttcctcggta tcgttaagta cccaaacatt gaacgcggat
     1501 tcccattcaa ccgccacact gacttttatt gcgcatgatg cagcaggtaa tcctgttgtc
     1561 gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca tcaccctttc tgaatggaaa
     1621 gataatggtg acggaagcta tacccagatc ctgaccacag gagcgatgtc tggcacgctg
     1681 acgctgatgc cacagctgaa tggtgtggat gcggctaaag cccccgccgt ggtgaatatc
     1741 atttctattt cgtcatcccg aactcactcg tcaattaaaa ttgataaaga ccgttatctc
     1801 tccggcaatc ctatcgaggt gacggtagaa ctgagagatg aaaatgacaa acctgttaag
     1861 gagcaaaaac agcaactgaa taacgcagtc agcatcgaca acgtgaaacc tggtgtcact
     1921 acagactgga aagaaaccgc agatggcgtc tataaggcaa cctataccgc ctataccaga
     1981 ggcagtgggc ttactgcgaa gctgttaatg caaaactgga atgaagattt gcataccgct
     2041 ggatttatca tcgacgccaa cccgcagtca gcaaaaattg cgacattatc tgccagcaat
     2101 aatggtgtgc tcgccaatga gaatgcagca aacaccgtct cggtcaatgt cgctgatgaa
     2161 ggaagcaacc caatcaatga tcataccgtc acgtttgcgg tattaagcgg gtcggcaact
     2221 tgctttaaca atcaaaacac cgcaaaaacg gatgttaatg gtctggcgac ttttgatctg
     2281 aaaagtagta agcaggaaga caacacggtt gaagtcaccc ttgaaaatgg cgtgaaacaa
     2341 acgttaattg tcagttttgt cggcgactcg agtaccgcgc aggttgatct gcagaagtcg
     2401 aaaaatgaag tggtcgctga cggcaatgat agcgccacaa tgaccgcgac agtccgggat
     2461 gcaaaaggca acctgctcaa tgacgtcaag gtcactttca atgttaattc agcagcagcg
     2521 aaactgagcc aaaccgaagt gaatagccac gacgggatcg ccacagctac gctgaccagt
     2581 ttgaaaaatg gtgattatag ggttacggcc tctgtgagct ctggttctca ggctaatcaa
     2641 caggtgattt ttatcggtga tcaaagtact gctgccctga ccctcagtgt gccttcaggt
     2701 gatatcaccg tcaccaacac agctccgcta catatgactg caaccttgca ggataaaaat
     2761 ggcaacccat taaaagataa agaaatcacc ttctctgtgc caaacgacgt cgcaagtcgg
     2821 ttctcgatta gcaacagcgg aaaaggcatg acggatagca acgggactgc aatcgcctcc
     2881 ctgaccggca cgttagcggg cacgcatatg atcacggctc gtctggctaa cagcaatgtc
     2941 agcgatacac agccaatgac gtttgtggcg gataaagaca gagcggttgt cgttctgcaa
     3001 acatcgaaag cggaaatcat tgggaatggc gtggatgaga caactctgac agcaacagtg
     3061 aaagatccgt cgaatcatcc ggtggcgggg ataacggtca acttcaccat gccacaggac
     3121 gttgcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc caatggggaa
     3181 gcgcatgtca cgctgaaagg taaaaaagcg ggcacgcata cggttaccgc aacgctgggt
     3241 aataacaata ccagtgattc gcagccggta acatttgtgg cggacaaagc ctcggctcag
     3301 gttgtcctgc agatatcaaa agatgagatc acaggtaatg gcgtcgatag cgcaacgcta
     3361 actgcaacgg ttaaagatca gttcgacaat gaggtgaata atcttccggt aacattcagc
     3421 tcagcctctt caggactcac cctgaccccg ggagtgagta ataccaatga gtctggcatc
     3481 gcgcaggcca ctatcgcagg cgttgccttt ggtgagcaga cggtcacggc atcactggct
     3541 aataatggtg ccagcgacaa caaaactgtg cattttattg gcgacacagc ggcggcaaaa
     3601 attatcgagt tgacgcctgt cccagacagc ataatcgccg gtaccccgca gaacagcacc
     3661 ggcagcgtca tcaccgccac agtcgttgat aataatggct ttccggtgaa aggtgtgact
     3721 gtgaacttca ccagccgaac aaactctgcc gaaatgacga atggcggcca agccgtaacg
     3781 aacgaacagg gtaaggcaac cgtcacttat accaataccc gctcctcgat agaatcagga
     3841 gcgagaccgg ataccgttga ggccagtctg gaaaatggta actccacgct tagcacatca
     3901 attaatgtca acgcggatgc gtctacagca catctcacct tgctacatgc actttttgat
     3961 acagtctccg caggcgagac aaccagtctg tatattgagg tgaaggataa ttacggcaat
     4021 ggtgtacccc agcatcaggt aaccctcagc gtttcaccaa gtgaaggcgt gaccctcagt
     4081 aataacggta tatatactac caactactac ggctattttt acgcaagctt taccgctaca
     4141 aaagccgggg tttatcaagt gacggcaacc ctcgacaatg gcgattcgat gcaacaaaca
     4201 gtgacctatg tgccgaacgt cgcgaatgct gaaatcacgc tggcagcctc gaaggatccg
     4261 gtgattgccg acaataacga tctcacaaca ctaacagcaa cagtcgctga tacagagggc
     4321 aatgcgatag ccaacactga ggtaacattt actctgccgg aagatgtgag ggcgaacttc
     4381 acgctgagcg atggcggtaa agcgattact gatactgaag gcaaagcgaa agtcacgctg
     4441 aaaggtataa aagcaggcgc tcatactgtt acagcatcga tggctggcag taagagtgga
     4501 caactggtgg tgaactttac tgcggatacg ctcactgcgc aggttaatct taacgttacc
     4561 gaggacaatt tcatcgccaa taacattggg atgaccaagc tgcaggcaac agtgactgat
     4621 ggaaacggca acccattcgc caatgaggcg gtgacattca cgctaccggc agatgtgagc
     4681 gcaagcttca ctctcggaca aggcggttcc gccattactg atatcaacgg caaggctgaa
     4741 gttacactga gcggtaccaa atccggcacc tatcccgtga cagttagcgt gatcaattat
     4801 ggtgtcagtg atacgaaaca ggtgactttg attgccgatg ctggtaccgc aacactagcc
     4861 tccttaacct ctgtatactc attcgtcgtt agcacgaccg agggcgcgac catgactgca
     4921 agcgtcactg acgctaacgg caacccggta gaaggcataa aagttaattt ccgcggaacc
     4981 tccgtcacgc taagcagcac cagcgttgaa acggatgatc agggtttcgc tgaaattctt
     5041 gtgacaagca ccgaagtcgg actgaaaaca gtttcagcct ctctggcaga taaacctact
     5101 gaagtcatat cgcgattact gaatgcaaaa gcagatatta attctgcaac gattaccagt
     5161 ctggagatac ctgaaggtca gctaatggtc gcacaagacg tagcagttaa agctcacgtc
     5221 aacgaccagt ttggcaaccc gattcttaat gaatctgtaa cattcagtgc agagccacca
     5281 gagcacatga ccatcagcca aaatattgtc tctactgata cgcatggtat agccgaggtc
     5341 tccatgacgc ccgaaagaaa cggttcgtat atggtgaaag catccctggc gaatggagcc
     5401 tcacttgaga aacaactgga ggctattgat gaaaaactga cactcacggc gtccagtccg
     5461 cttatcggtg tctatgcccc tacaggcact actctgacgg caacgctaac ctctgcaaat
     5521 ggcactccag tggagggtca ggtcatcaac tttagcgtaa cgccagaagg ggcgacgtta
     5581 agtggcggaa aagtgagaac taactcttca ggtcaggctc cggtcgttct gaccagcaat
     5641 aaagtcggta catatacggt gactgcatcg ttccataacg gcgtaacaat acagacacag
     5701 acaaccgtga aagtcactgg caactcaagc accgcacatg ttgctagctt tatcgctgat
     5761 ccatcgacta tcgccgccac caacagtgat ttaagtacct taaaggcaac ggttgaggat
     5821 ggcagtggta acctgatcga aggtctcact gtgtacttcg ccttaaaaag cggctctgcc
     5881 acattaacgt cattaacagc ggtgaccgat caaaacggaa tcgcgacaac aagcgtgaaa
     5941 ggagcgatga caggtagcgt cacggtaagc gcagtcacga ccgctggtgg aatgcaaaca
     6001 gtagatataa cgctggtggc aggcccggca gacgcctcgc agtccgtcct taagaacaat
     6061 cggtcctcac tgaaagggga ctttaccgat agtgctgagc tacatcttgt tttgcacgat
     6121 atatcaggca atccgatcaa agtttctgaa gggatggaat ttgtgcaatc aggtactaac
     6181 gtgccctata tgaaaattag cgcaattgat tacagtcaaa atatcaacgg tgattacaaa
     6241 gccaccatta caggcggcgg agagggtatc gcaacgctga tccctgtatt gaatggtgtt
     6301 catcaagctg gtctgagtac cacaatacaa ttcactcgcg cagaagacaa aataatgagc
     6361 ggtacagtat cagtcaatgg tactgaccta ccgacaacta cattcccttc gcaggggttc
     6421 accggggcgt attatcagtt gaataatgac aactttgccc caggaaaaac ggcggctgat
     6481 tatgagtttt caagctctgc ctcctgggtc gatgttgatg ctaccggtaa agtgacattt
     6541 aaaaatgtcg gcagcaattg ggaaaggatt acggcgacgc caaaatcagg aggccctagc
     6601 tatgtatacg aaatccgtgt gaagagttgg tgggtgaact ccggcgatgc tttcatgata
     6661 tacagccttg ctgaaaattt ttgcagtagc aatggctaca cacttccccg tgcagatcat
     6721 ttaaaccata gtcgttcccg aggcatcggg tcactgtaca gtgaatgggg agatatgggg
     6781 cattacacga ctgaagctgg ttttcaatca aatatgtatt ggtcatctag tcccgcaaac
     6841 tcaagcgaac aatacgtagt ttccctggca acaggtgatc aaagcgtatt tgaaaagctt
     6901 gggtttgctt atgcgacatg ttataaaaac ctgtgatttt ccgttataat ttcttaaaga
     6961 gtgctccatt cggagcactc ttttttacat tccctctata gagcttgccc ttctctcaca
     7021 gaaaatgaat aaataaaact gctgcaccgc aagaatcgcg ttcgatgtga ctatgatcgt
     7081 atccggtgag tacagctttg atccaaaggc tggttttctg tgatgattga ccagcctcag
     7141 agtattgatg acaccaccgt tcataacaga tcggcattac tatgcataag tactgtctca
     7201 tattctcgcc aaaacacttt ttaatgcctt tgcgaattca tccagactta tattgtcaat
     7261 tttttgccca acgacgactg actccgaaac atcggccatt gcgggccatc gcgttttgta
     7321 atcagcataa attgcaacaa cgggagtatt ataggcactt gccatatgta atattgaggt
     7381 gtcaggagtt attgcgatat aagcatcttt tatgattgct gctgaacgtt taatcgaggg
     7441 tgataaggat aaacggtaga cattattata acaatccact aataccctgg ctttatcttc
     7501 cccttttggt ccatgaacga tcactattgg tatatctgtt tcactttgaa tttttgctat
     7561 tagattttct gcaatcgata atgaaaatgt acgttcctgc gagctacctt ctaaattaag
     7621 cgcaatgtaa gatcctaacg atcgcatttc ctcgcgcacc tcatcgagta catcctcact
     7681 tagtggcaat tcatatattg gcctgactgc tggaaaaccc gcctcacgca tcataaatgc
     7741 ccatgtcatg ggtacaggag cccggagatt ctgatccatc cgggacgcgt tcttgcacaa
     7801 aggggagtag cacttcatgg ttaaaccaac aacctgaaaa ttcgtttttg ctttcaactg
     7861 actgataaat atcatcgttt tcaggttctt tttacgcatc gcctctatac aaagatccgg
     7921 cgtaccgtat ttctgtgtta tgttctttgc taaatctttt atttctttta atgttgcgtg
     7981 atcctgcata gtcattgtga ctaatgttaa tttagtcttt tcaagtttaa gtgcattaaa
     8041 cacttctaaa ttaattgtcg acgtaacaat taaaagatgc ttaattttat gcaattcaag
     8101 cgcccgaata acaggaaaga tggccatagc atcgccaatc tggtcgggaa tatggatgac
     8161 aacaaagtct gttttttcaa tattgaaatt ataagcttta taatcgtagt aactaaatgc
     8221 aatacgtctc aacaatgatg ctaaaaacat acctaacctc gcctccctac tggttataat
     8281 gcaatgcagt ctatcagact catcagggtg ccattttgtg catatgcgga cttttatgtt
     8341 tcatatctct aacctgtggc tcctctgctt aatccttaaa caacaccagc aactcctgcg
     8401 ctttcatctt ccatcgaatt tttcatgttg ccgctaatca gccataaaat catttgcaga
     8461 tgcgctctgt cgaggtagtc tcataaggtt cgtttataga tcgacggcaa tgtgagttac
     8521 cttttccata ctaattataa aaagacagta caaacaggat cattatggac tccacgctca
     8581 tctccactcg tcccgatgaa gggacgcttt cgttaagtcg cgcccgacga gctgcgttag
     8641 gcagcttcgc tggtgccgtc gtcgactggt atgatttttt actctatggc atcaccgccg
     8701 cactggtgtt taatcgcgag tttttcccgc aagtaagccc ggcgatggga acgctcgccg
     8761 catttgctac ctttggcgtc ggattccttt tccgtccgct cggcggtgtc attttcggtc
     8821 actttggcga ccgactggga cgtaagcgca tgttaatgct gaccgtctgg atgatgggca
     8881 tcgcgacagc cttgattggt attcttcctt cattctcgac cattgggtgg tgggcaccta
     8941 ttttactggt gacgctgcgt gccattcagg gatttgccgt cggcggcgaa tggggaggcg
     9001 cggcgttgct ttccgttgaa agtgcaccaa aaaataaaaa agccttttac agtagcggtg
     9061 tacaagttgg ctacggtgta ggtttactgc tttcaaccgg actggtttca ttgatcagta
     9121 tgatgacgac tgacgaacag tttttaagct ggggctggcg cattcctttc ctgtttagca
     9181 tcgtactggt actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg
     9241 aacaacagca acattatcaa gctgccgcga aaaaacgcat cccggttatc gaagcgctgt
     9301 tacgacatcc cggtgctttc ctgaagatta ttgcgctacg actgtgcgaa ttgctgacga
     9361 tgtacatcgt tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg
     9421 aacttttcct caatattggt ttgctggtag gtggattaag ctgcttgaca attccctgtt
     9481 ttgcctggct tgccgatcgt tttggtcgcc gcagggttta tatcacaggt gcgttaatcg
     9541 gaacgttgag cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag
     9601 ttttcttctc cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac
     9661 cgatgtttac cgaaatgttt ggtgccagtt atcgctatag tggtgctgga gtcggttatc
     9721 aggtcgccag tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt
     9781 actttgccgg gaactggcat agcgtcgcca tttatttgct ggctggatgt ctgatttccg
     9841 caatgaccgc tttgttgatg aaagacaatc aacgcgcttg atagtctggc gaagatcatc
     9901 cgatcttcgc cttacacttt tgtttcacat tcctgtgaca tactatcgga tgtgcggtaa
     9961 ttgtatggaa caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc
    10021 actggataaa ctcgacgcgc tgtatgagca atctgtagtc gcattacgca acgccattgg
    10081 caactatatt acaagtggcg aattacctga tgaaaacgcc cgcaaacaag gtctttttgt
    10141 ctatccatca ctgaccgtaa cctgggacgg tagcacaacc aatcccccca aaacgcgcgc
    10201 atttggtcgt tttactcacg caggcagcta caccaccacg attactcgcc ctactctctt
    10261 tcgttcgtat ctcaatgaac aacttacgtt gctgtatcag gattatggtg cgcatatctc
    10321 ggtgcaaccc tcgcagcatg aaatccccta tccttatgtc atcgatggct ctgaattgac
    10381 acttgatcgc tcaatgagcg ctgggttaac tcgctacttc ccgacaacag aactggcgca
    10441 aattggcgat gaaactgcag acggcattta tcatccaact gaattctccc cgctatcgca
    10501 ttttgatgcg cgccgcgtcg atttttccct cgcgcggttg cgccattata ccggtacgcc
    10561 agttgaacat tttcagccgt tcgtcttgtt taccaactac acacgttatg tggatgaatt
    10621 cgttcgttgg ggatgcagcc agatcctcga tcctgatagt ccctacattg ccctttcttg
    10681 tgctggcggg aactggatca ccgccgaaac cgaagcgcca gaagaagcca tttccgacct
    10741 tgcatggaaa aaacatcaga tgccagcatg gcatttaatt accgccgatg gtcagggtat
    10801 tactctggtg aatattggcg tgggaccgtc aaatgctaaa accatctgcg atcatctggc
    10861 agtgctacgc ccggatgtct ggttgatgat tggtcactgt ggcggattac gtgaaagtca
    10921 ggccattggc gattatgtac ttgcacacgc ttatttacgc gatgaccacg ttcttgatgc
    10981 ggttctgccg cccgatattc ctattccgag cattgctgaa gtgcaacgtg cgctttatga
    11041 cgccaccaag ctggtgagcg gcaggcccgg tgaggaagtc aaacagcggc tacgtactgg
    11101 tactgtggta accacagatg acaggaactg ggaattacgt tactcagctt ctgcacttcg
    11161 ttttaactta agccgggccg tagcaattga tatggaaagt gcaaccattg ccgcgcaagg
    11221 atatcgtttc cgcgtgccat acgggacact actgtgtgtt tcagataaac cgttgcatgg
    11281 cgagattaaa cttcccggcc aggctaaccg tttttatgaa ggcgctattt ccgaacatct
    11341 gcaaattggc attcgggcga tcgatttgct gcgcgcagaa ggcgaccgac tgcattcgcg
    11401 taaattacga acctttaatg agccgccgtt ccgataataa gttctgaacc ttcttatcag
    11461 aaatagatgt gaaggagtaa gtaagacacc tggcaaatag cctgcaataa tcgtgggctg
    11521 tttgcttcct tgggcggata cgtgttttat tatcgtctta atgatttcca catattaaaa
    11581 gcaagtatgc tttcaaaaca
//
LOCUS       DAZFWP020000149        11555 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFWP020000149 DAZFWP020000000
VERSION     DAZFWP020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.149"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..467)
                     /locus_tag="ACHK0Z_RS19715"
                     /old_locus_tag="pgaptmp_003943"
     CDS             complement(<1..467)
                     /locus_tag="ACHK0Z_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HGU4503884.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYD"
     gene            complement(1264..1476)
                     /gene="yncH"
                     /locus_tag="ACHK0Z_RS19720"
                     /old_locus_tag="pgaptmp_003944"
     CDS             complement(1264..1476)
                     /gene="yncH"
                     /locus_tag="ACHK0Z_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HGU4503885.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            complement(1552..2169)
                     /gene="yncG"
                     /locus_tag="ACHK0Z_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             complement(1552..2169)
                     /gene="yncG"
                     /locus_tag="ACHK0Z_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU4503886.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2436..3935
                     /gene="ansP"
                     /locus_tag="ACHK0Z_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             2436..3935
                     /gene="ansP"
                     /locus_tag="ACHK0Z_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU4503887.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(4048..5109)
                     /gene="yncE"
                     /locus_tag="ACHK0Z_RS19735"
                     /old_locus_tag="pgaptmp_003947"
     CDS             complement(4048..5109)
                     /gene="yncE"
                     /locus_tag="ACHK0Z_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU4503888.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5351..7453
                     /gene="pqqU"
                     /locus_tag="ACHK0Z_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             5351..7453
                     /gene="pqqU"
                     /locus_tag="ACHK0Z_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU4503889.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATSRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7489..8154)
                     /gene="mcbR"
                     /locus_tag="ACHK0Z_RS19745"
                     /old_locus_tag="pgaptmp_003949"
     CDS             complement(7489..8154)
                     /gene="mcbR"
                     /locus_tag="ACHK0Z_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU4503890.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(8352..9389)
                     /gene="curA"
                     /locus_tag="ACHK0Z_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             complement(8352..9389)
                     /gene="curA"
                     /locus_tag="ACHK0Z_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU4503891.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            9570..10088
                     /gene="mddA"
                     /locus_tag="ACHK0Z_RS19755"
                     /old_locus_tag="pgaptmp_003951"
     CDS             9570..10088
                     /gene="mddA"
                     /locus_tag="ACHK0Z_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU4503892.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            10085..10534
                     /gene="ydcZ"
                     /locus_tag="ACHK0Z_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             10085..10534
                     /gene="ydcZ"
                     /locus_tag="ACHK0Z_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4503893.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(10535..10768)
                     /gene="ydcY"
                     /locus_tag="ACHK0Z_RS19765"
                     /old_locus_tag="pgaptmp_003953"
     CDS             complement(10535..10768)
                     /gene="ydcY"
                     /locus_tag="ACHK0Z_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU4503894.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(10854..11027)
                     /gene="ortT"
                     /locus_tag="ACHK0Z_RS19770"
                     /old_locus_tag="pgaptmp_003954"
     CDS             complement(10854..11027)
                     /gene="ortT"
                     /locus_tag="ACHK0Z_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU4503895.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            11222..11317
                     /gene="yncL"
                     /locus_tag="ACHK0Z_RS19775"
                     /old_locus_tag="pgaptmp_003955"
     CDS             11222..11317
                     /gene="yncL"
                     /locus_tag="ACHK0Z_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU4503896.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2907 a         2777 c         2744 g         3127 t
ORIGIN      
        1 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
       61 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
      121 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
      181 tgcaccttca tgctgtacag catctggttt ttgtcgttct ccccgagttc aaaccaggtc
      241 accacgcctt tcacccggcg ctgtacgtca tcgccctgcc atatcgtcag gtaggccatt
      301 ttgtccagca cctgcgcaaa ttcaagggag agaaactgct ggctgaccag ggagagatcg
      361 agggaaaaga gcgaagagag tgactggttc agatgaaagg aaaccaccgc aaaagcgtcc
      421 ggcggcaggc cgtccacttc cagtgtgaaa cgtaatccgg ttgacatagc aacctccagt
      481 aaaatgaata atgatccgta ccgtacaact gcggataatg tgataagcgg gacgacttca
      541 gacgttatta gggaaggaac agaaaatgct atggttatgt gagaattttg tttaaatatt
      601 actttacaga aacgggtgtc tgttcagtca aaaaaataat cacaagctca acctcttgtt
      661 tcttggcgtt attcaggaca ggtacgcata tcacaacgag taacaaaaac gcaaagttaa
      721 accaacattc ataatatatt tttattcaca gcaataaata atccaattaa cattattaaa
      781 atgaatgatt gcataagaaa tatatgctac cgctccaccc tgataacatg atctgcacat
      841 tgccatctta acgtcgttaa acactggctt aatagtggat atttcaaatt aggaatttat
      901 taaattcacc tgatgaatat cataagcaaa tagcatagaa aataatgaca attcccccga
      961 ggggaccatt tccaaagcac tgcaccacca cctggtgccc ggatgatctt gcttcagtgg
     1021 taagagtacc tgattcattg ttcccaaaat gatgttttcc ccggccccgc caataatgtg
     1081 cagctctcac gtgataatct attgattagg ttatattaag aatcaacatt taaaatacat
     1141 ccattttctt gaataaatca acatgtttat taatcaattg ctgttttagc tctgactcat
     1201 caagctctcc atatttgtcc tacagataat tgcgaataaa tacagagaac gccatttatc
     1261 cttttagcat acctgccgga tatttttatt tgtattaata ttaattgcat ctccaactcc
     1321 gagcatatcc atcattaaaa actttttgag attatagaat acctgtgata acaagtgaaa
     1381 catctgcaca atgtgaatag ttagtttctt ttcagaggaa atgaaataga caagctgaat
     1441 gaaggggaga gtgatataaa tcaaaaaaca taacatgaat ttaattaatt acgcccatca
     1501 ttgtggataa agttactgaa taataaataa cataatcctc tggtatccca ctcaaataat
     1561 ttcattcctt tttaatactt cttgtaattt cggtaactga catacagcat cagcaatcgc
     1621 actgatattc gtggcattat cctgaaacca ttcatgccca ggcccccatg tgcgcatagt
     1681 gcaaagataa cagtccacca gcgttagttg ctcaccaaac gcataaggtt cagcggtcaa
     1741 ttgtgaattc agccagatat aaagcgattt acgatattca ataacattct tctttaactg
     1801 ttcaggtgcg tcaggagccc aacgctcagg gtaatcggcg aaagtgaatg ttggatagac
     1861 attagcaacc agccagacca atagccgttg aaactgctga cgttcggcgc gcccaaccgg
     1921 tggcgcaagg tccgggcgac gatcgagaac catcaatgca atcgccgctg tctccgtcat
     1981 aatctcgtca ttttccagcg ccagtgtcgg aatctgacac aacgggttca gggttttcaa
     2041 taactcgcgt gaagttccct cgtggtcaaa accactgaca tcaacgaact gataaggaat
     2101 atcagccagt gttagcatca gctcactgat tgtagagccc catccgggta cgccatacac
     2161 cttaatcata tgcccctcac aatcttttaa agattaagtg taggacagta cacaggttaa
     2221 ccagcaaaag gatttgtcat tttttcgtca gttgtattaa tccccttcag gattgtaagg
     2281 ggatgttttg catcgtttat cgataacggg taggatgctg cgccgatagt tttataccca
     2341 taaagaataa tggtgataac tatcatcgcc aggatgaata aacattgttc atggcaactt
     2401 atatgacttt ttcattaaag caatcaggga gagcaatgag taaacacgac accgacactt
     2461 cagatcaaca cgccgcgaaa cgccgctggc ttaatgccca cgaagagggg tatcacaaag
     2521 cgatgggcaa tcgccaggta cagatgatcg ccattggcgg cgcgattggc accggcttgt
     2581 ttttaggtgc aggagcccga ctgcaaatgg cgggtccagc actggcactg gtttatttaa
     2641 tttgtggctt gttttcgttt tttattctgc gtgcattggg tgagctggtg ctacaccgcc
     2701 cttccagtgg cagttttgtc tcttatgccc gtgagttttt gggtgagaaa gccgcttatg
     2761 ttgctggctg gatgtacttc atcaactggg cgatgacggg gattgttgat attaccgccg
     2821 tcgctctgta tatgcattac tggggtgcgt ttggcggcgt gccgcagtgg gtctttgcgc
     2881 tcgctgcgct gaccatcgtt ggcaccatga atatgatcgg tgtgaaatgg tttgcggaga
     2941 tggagttctg gtttgcgctt attaaagtgc tcgccattgt gacctttttg gtcgtgggta
     3001 cagtgttcct cggtagcggt cagccgctgg atggcaacac cactggcttt catttaatca
     3061 ccgataatgg cggcttcttc ccccacggtt tgctgcctgc gctggtgttg attcagggcg
     3121 tagtgtttgc ttttgcctcc attgaaatgg tgggtacagc tgccggagaa tgtaaagatc
     3181 cgcagaccat ggtgcctaaa gcgattaaca gtgtgatttg gcgtattggc ctgttttacg
     3241 tcggctccgt ggtgttgctg gttatgttat tgccgtggag cgcgtatcag gcggggcaaa
     3301 gtccgttcgt gacgtttttc tctaaactgg gtgtgccata tatcggcagc attatgaaca
     3361 ttgtggtgct gaccgctgcc ctctccagcc tgaactcagg cctgtactgc accgggcgta
     3421 ttctgcgctc gatggcgatg ggcggttccg caccgagttt tatggcgaaa atgagtcgtc
     3481 agcatgtgcc gtatgccggg attctggcga cactggttgt gtatgtcgtc ggcgtattcc
     3541 tcaactatct ggtgccgtcg cgcgtatttg agattgtgtt gaacttcgcg tcgctgggaa
     3601 tcatcgcttc atgggcgttt atcatcgtgt gccagatgcg cctgcgtaaa gcaattaaac
     3661 aaggcaaagc agcggatgtc agttttaaac tgcctggcgc gcccttcact tcctggctga
     3721 cattactgtt tttactgagt gtcctggtgc tgatggcgtt cgattacccg aacgggactt
     3781 atactatcgc ggcgctgccg attatcggta ttctgctggt tataggctgg tttggtgtgc
     3841 gcaaacgcgt tgctgaaatt cacagcactg cgccagtcgt cgaggaagat gaagaaaaac
     3901 aggaaattgt gtttaagcct gaaacggcga gttaattgca aaattgcccg gctacgtgcc
     3961 gggcaaattc agattactga cgaaggtgcc ggatgcggcg tgataaaagt caggcaagtc
     4021 tctccgtttt caccctcgcc atatctatta cagcgcaata cgaatcacat cgtctggctg
     4081 ggtagcttcc tgctgtttag tggatttttg tttcacactg acatacagcg ttttgccatc
     4141 ggcagacagc gccaggctgt ttggatgagt cggcgtatcg aacgttttca ccactttata
     4201 gcttttcgcg tcaatcacac taactttacc tgcctgacga tgcgttacgt aggcttcatt
     4261 acgcgctggg ttaaacagca cagccagtga ttccggtgcc gcaaccttcg ccagaatatt
     4321 gccattacgg gtatcgacca ctaacacttc tgcggcttta gaatcggtga taaatgcacg
     4381 ctgcctggcg gtatcaaggc taatgttgat aaagaagtgc tctttgccgt catccagcag
     4441 ctttttacgg ctgaggattt tattgtcggc ggtgtcgatg gtaatcaatt cgccgtcagc
     4501 gttagtggtg taaagacgtt tgcctttgct atccagcgcc agaccggtac tcattttacc
     4561 ggtgttctgg atggcggttt tcagtttgat attctcgcca tcaacgaccc aaatcacgct
     4621 atctttacca ataccactga tgtaaacggt gttcgtggca tcatcagcta ccagctcacg
     4681 cggttgcagc gggcgcacct cttccgtgcg cttacgatca tccagcacca gacggccttt
     4741 cacttcgccc gttttggcat ctatcgccgt gaccgcgctg tttacggtgt taccaaacca
     4801 caacgtctga gtcgtgttat tgatggtggc accaaacggc ttgagatcgt tatggatcgc
     4861 ctgcgtcact tccagagtga ccggatcaag acgataaacc acgccacctt tatccagttt
     4921 gcggctttgc gaagtggcga gccacagcgc gttttcttgc tggctataag ccatttcgta
     4981 ggcaccttta cctaccgctt tacgcagcat ttcttctgcg gcctgcgtac tgaatgatga
     5041 agcaacaagc aatgaaccta acagtaatga accacgcagg cgcgatgaaa acagatgacg
     5101 taaatgcatg acgactccct ttgatgaacc aatatttttg ctgcttcaca atgcttcgct
     5161 aagcgtaatc atcgttacgc tcttgttata aatgggaatg gtaatcatta ttttctcaaa
     5221 tgtggagcat ttcttgccgt tacgactaca attttctcaa ttaattaacg tatcgtgccg
     5281 tttcgttttc cataaattta caattttatt accatgacag cgttttccca ttcttaacct
     5341 ggcttttttc atgaagattt tttccgtccg acagaccgtt ttgcccgcac tgcttgtcct
     5401 ttcccccgtt gtttttgccg ctgatgaaca gactatgatt gtcagtgccg caccgcaggt
     5461 ggtttcagaa ctggataccc cagcagcagt aagcgtggtg gatggcgagg agatgcgcct
     5521 ggcaacatcg cgcattaact tgtccgaatc actgaccggc gtgcctggtt tgcaggtaca
     5581 aaaccggcag aactatgcgc aagatttaca gctgtcgatt cgcggatttg gctcccgctc
     5641 cacttacggt attcgcggta ttcgcctgta tgtggacggt attcccgcca ccatgcccga
     5701 cgggcaaggg caaacatcca acatcgattt aagcagtgtg caaaatgtgg aagtgctgcg
     5761 tggccccttc tctgccctgt atggcaacgc gtctggtggg gtaatgaatg tcaccaccca
     5821 gaccggacaa cagccaccaa ccattgaagc cagtagttac tacggcagtt ttggcagctg
     5881 gcgctatggg ctgaaagcaa cgggcgcaac gggagacggc acacagcctg gcgatgtcga
     5941 ttacaccgtc tcaaccacgc gttttacgac ccacggctat cgtgaccata gtggcgcaca
     6001 gaaaaattta gctaatgcca aactgggcgt acgcattgat gaagccagca aattaagtct
     6061 gattttcaat agtgtggata tcaaagcaga tgacccaggt gggctaacca aagcagaatg
     6121 gaaggctaat ccacaacaag cgcctcgtgc agaacagtac gatacgcgaa aaaccatcaa
     6181 gcaaactcag gctgggttgc gctatgagcg tagcctgagc gcgcaagatg atatgagtgt
     6241 gatgatgtat gccggagagc gagaaacgac ccagtaccag tcaataccca tggcaccaca
     6301 acttaacccg tcacatgcgg gcggcgtgat taccctgcaa cgccattacc aggggataga
     6361 cagccgctgg acacaccgtg gtgaactggg cgttccggtc acgttcacta ccggcctgaa
     6421 ctacgaaaac atgagtgaaa accgcaaggg ctacaataac ttccgcctga atagcggcat
     6481 gccggagtac gggcaaaaag gtgagttgcg tcgcgacgaa cgcaatctga tgtggaacat
     6541 cgatccctat ttacagacgc agtggcagct gagcgaaaaa ctgtcgctgg atgctggcgt
     6601 gcgctacagc tccgtgtggt ttgattccaa cgaccattac gttactccgg gtaacggcga
     6661 tgacagcggt gatgccagtt atcataaatg gctacctgcc ggttcgttaa aatatgcaat
     6721 gaccgatgcc tggaatatct atctggcagc cgggcgaggt tttgaaacgc cgacgattaa
     6781 tgagctgtct tatcgtgctg atgggcaaag cggtatgaac tttggtttaa aaccatccac
     6841 caacgataca attgagatcg gcagtaaaac gcgtattggt gatgggctgc ttagtctcgc
     6901 attgtttcag accgacactg atgatgaaat tgttgtcgat agcagtagcg gtgggcgtac
     6961 gacttacaaa aatgccggaa agacccgtcg tcaaggcgct gaactggcat gggatcaacg
     7021 tttcgcagga gattttcgcg taaacgcgtc ctggacctgg cttgatgcga cctatcgcag
     7081 caatgtttgc aatgaacagg attgtaacgg taatcggatg ccagggatcg cccgtaatat
     7141 gggctttgcg tcgataggtt atgtaccgga agatggttgg tatgcaggca cggaagcgcg
     7201 ttatatgggc gatattatgg cagatgatga aaatacggca aaagcgccgt cttatactct
     7261 cgtcggctta ttcaccgggt ataaatacaa ttaccacaat ttaactgtgg atttatttgg
     7321 tcgtgtcgat aatttattcg ataaagaata cgttggttct gtcattgtca atgagtcaaa
     7381 cgggcgatat tacgaacctt cgcccggacg aaattatggt gtcggcatga atattgcgtg
     7441 gagatttgag taattacctt gatgcccggt atttgccggg catttacttt aacgattgta
     7501 ttgctggtat aaaatagcaa tattttgttg cataatttca gcaagacaat gtctgcatcc
     7561 ctctttgtct tttgctttta acgcagcgag taacagatga tagttttcta tatgctcccg
     7621 taattccgct ggattaatcg cttcatagag ataatgtaaa ccaggcccca tcctgaccca
     7681 cagttgctca atcatctcac acaggatggg catatttgag cgatgataaa tcgctaagcg
     7741 aaatagcctg tttacattaa tgatttgttc catgtcgccc ttttcttgcg cctgctgtaa
     7801 tttctcgagc aattcctgga gttccgccag gtcttgcggg gtgaggtttt caacagccag
     7861 agcaactgcc attaattcca gttcgtagcg gatccgattg atttcgtcta attgacgttt
     7921 cctcacttcc ggaactgtaa atgcttgtgc aggtgcgaca gaaagcgcat tcaccgaaac
     7981 cagacgtaat aatgcttcac gcacaggtgt aatactcata cctaattgct ccgccagatt
     8041 tttagtaatc aggcgtgcgc caggttttaa tgcgccaata ctaagctgat gtttcaggtc
     8101 gttctcaacc tgcagagtca aactgacatg tttcattttt cccattccag gcattttaat
     8161 tccttcgcgt gttttctgtc caaccctgcc tgcacggcag aaggagttca cctggtgtat
     8221 taaagtgaat aatcataaac cacccacctt tgttgttatt agcagcagaa caatgacgtg
     8281 gttagttaat taaagctgaa aataagcttt ttgcctggat ttttatggcg gcttacgccg
     8341 ccgttgaaac tttaatcatc acccgcgacg cggatcacca ctttgccgaa gtttttaccc
     8401 ttcagcaggc cgataaacgt ctgtggcgca ttctctaaac catcagtaat atcttcgtgg
     8461 tagtggattt tatcctcttt cacccattgc cccatctcct tctgaaactc atggatgcgg
     8521 tgaccataat cctgagcgat aataaaccct tgcaagcgaa tacgtttttt cagcaccgtt
     8581 gccatcaaca ggggtaaacg atccggaccg ggtggtagct ctgtagcgtt atagctgctc
     8641 actaagccac aaacgggaat gcgcgcagat gtattaagta atggtagcac tgcatcaaat
     8701 accttaccgc ccacgttttc ataatagata tcaatacctt ttgggcacgc tttcaccagt
     8761 tgttcggcaa aatcatccgc gtggtgatca agacaaacat cgaagcccag cacctcgata
     8821 gcatggcggc atttttccgc gccaccggca acccccacca ccctgcaacc tttaagtttg
     8881 ccgatttgcc ccaccgttgc tcccacagga cctgtcgccg cagctaccac caacgtttcg
     8941 ccctctttag gctgaccgat atccagcagc cccatataag cggtaaagcc tggcatcccc
     9001 agcacaccca gcgaccacga tggattttgc ggatgatcgc caagtttcac cagatcatca
     9061 ccactggata tgtcatagtc ctgccatcca ctgtagccca gcacccagtc gccaggctga
     9121 taatcaggat gattcgactc cacaacacgg ctcaccgtgc cgccgaccat cacgccgcca
     9181 atatcaacag gcggtgaata agatggctca tcgctcatac gtccacgcat atacgggtcc
     9241 agggacaaat aaactgtgcg cagtaacacc tgtccttcac ccggtgtggc gacatcatct
     9301 tcttcaagac ggaaattctc cggaaccggt gcgccatgtg gacgcgaggc cagaacccaa
     9361 cggcggttac gctgcttttg ttgccccata atgctctcct gtgcttttgg catgaatcca
     9421 tcaatagtag caggctcgca ggattggttt tgtttgcagc cgtcacatcc cctctgcaac
     9481 acgcaataat ttcatcgagg aatccgttgc ttcgggctgt tttcgatagt attgataaca
     9541 tcatcatcat tattttattg aggccagaca tgtccatccg ttttgcccgc aaagccgact
     9601 gtgctgccat tgcggaaatt tataaccacg ccgtgttgta tacggcggct atctggaatg
     9661 accaaacggt ggatgcagat aatcgcattg cctggtttga agcgcggacc atagcaggtt
     9721 atccggtgct ggtaagtgaa gaagatagcg tagtgacggg atatgcctcg tttggcgact
     9781 ggcgtagttt cgatggtttt cgccataccg tggaacattc ggtttatgtc catcccgatc
     9841 atcagggcaa aggtctgggg cgtaaattgt taagccgatt gattgatgaa gcgcgggatt
     9901 gcgggaagca tgtcatggtc gccgggatcg aatcgcaaaa tcaggcctcg ctgcatctgc
     9961 accagtcgct gggatttgtc gtcaccgcac aaatgccgca ggtgggcact aaatttgggc
    10021 gctggctgga tctgacattt atgcagttgc aactcgacga acgcactgaa ccggacgcga
    10081 ttggatgaat cagtcgctca cccttgcttt tttgattgcc gccggaattg gtctggtagt
    10141 ccagaacacg ctgatggtgc gcatcaccca gacctcctct accattctca tcgccatgtt
    10201 gctgaactca ctggtgggga ttgtgctctt tgtttctatt ttatggttta aacaaggcat
    10261 ggcggggttt ggcgaactgg tgtccagcgt gcgctggtgg acacttatac ccggcttgct
    10321 gggatcgttt tttgtcttcg ccagtatcag cggatatcag aatgtgggag cggcgacaac
    10381 tattgcagta ctggtcgcca gccagctaat tggcggtctg atgctggata tcttccgcag
    10441 tcatggcgta ccactacggg cgttgttcgg cccaatttgt ggcgcaattt tacttgtggt
    10501 tggcgcctgg ttggtagcca gacgctcatt ttaatcagag gatggtgccg cctttggtta
    10561 actgttcgtg gcgcgcttcc atatcttctt tatgcttgcg accgtgatga gcaatagcgg
    10621 tgcgcaaacg ctgctgctgg gtgtagcgat cttcccgact taactccgcg tcatcgctca
    10681 gggcgattaa taattcgttc atatgggcaa tgacgctctc ttcaatcgcg gcatccacgc
    10741 gcgcgatgac ttcatccaga tgcgacatac tttctcctta accacttgtg aaatttatcg
    10801 attcatccaa atgatataag tctgcgtggt gccgtcaagc cagtgagtaa gatttaaaac
    10861 agtgaaaaca acagcgccac cgggaaactc atcggccatg ttgcccccac cagaaaggcg
    10921 cttaagaaac ggatgcgttt cttatcgtga gaaagaaacc aggtgataag aaatgcgatt
    10981 gctgccataa ccgcataaaa aacaagcata tgttgataga gagacatgta cgggcgtcca
    11041 gccagatgaa taaaacgcgc gcaaatatgc gtcatttttt gacgtgcatc aagttttttc
    11101 accagtttga gaaaaaatca tcaaacagca acttgccctt atccatcttc agaatgagta
    11161 ttattgcgga ttttcttaac ccgtactata cacatagggg caatgataaa aggtggcaaa
    11221 aatgaatgtt tccagtagaa ctgtagtact gataaatgtc tttgctgctg ttggtttgtt
    11281 tactcttatc tctatgagat ttggctggtt tatttgatgt agtgattttg cacgcagcac
    11341 atactgtcag agaaatcaac tctctgacag tacgcaagat cttctcatac tctccaggtt
    11401 tacaacatta tccttaatat ttacttcttt ttgctggcat ctacattgaa atgagtagaa
    11461 aacatccccc tactgtcgtc tatacaaaca gcttactgat tcaaacggaa tcttgttttc
    11521 acatttttct atcatgaata ctttatacgc gcggg
//
LOCUS       DAZFWP020000150        11460 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFWP020000150 DAZFWP020000000
VERSION     DAZFWP020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.150"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..4481
                     /locus_tag="ACHK0Z_RS19780"
                     /old_locus_tag="pgaptmp_003956"
     CDS             <1..4481
                     /locus_tag="ACHK0Z_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HGU4503897.1"
                     /translation="EYSLVGVQDGSHGVVNVTDKGHWNFLGTGEAFRYIYIGDAGDGE
                     LNVSREGKVDSGIITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLV
                     VSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNIT
                     FLGDKASGIGTLNLMDATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG
                     KGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGELNITTGGIVKARDTQIALNDKSKGD
                     VRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAH
                     GEAAADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGH
                     TVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDY
                     TLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFAGVAQLKDSTFTLERDNTAALTHAM
                     LQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTW
                     KGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKAQTVIGSGG
                     SLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGG
                     QKLTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQMGTLRT
                     DADGALGNTRELNISNAAIVDLNGSTQTVETFTGQMGSTVLFKEGALTVNKGGISQGE
                     LTGGGNLNVTGGTLAIEGLNARYNALTSISPNAEVSLDNTQGLGRGNIANDGLLTLKN
                     VTGELRNSISGKGIVSATARTDVELDGDNSRFVGQFNIDTGSALSVNEQKNLGDASVI
                     NNGLLTISTERSWAMTHSISGSGDVTKLGTGILTLNNDSAAYQGTTDIVGGEIAFGSD
                     SAINMASQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAKTTIGGNLENGGTVQMNSE
                     GGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVG
                     AQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTP
                     PDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYIDSLHS
                     QGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAG
                     YANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVS
                     SDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEETLNTWYVQPQAQITWMGVKDSDHT
                     RKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKM
                     NGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            4690..4956
                     /gene="hipB"
                     /locus_tag="ACHK0Z_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             4690..4956
                     /gene="hipB"
                     /locus_tag="ACHK0Z_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGU4503898.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4956..6278
                     /gene="hipA"
                     /locus_tag="ACHK0Z_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             4956..6278
                     /gene="hipA"
                     /locus_tag="ACHK0Z_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGU4503899.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6590..6798
                     /locus_tag="ACHK0Z_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /pseudo
     CDS             6590..6798
                     /locus_tag="ACHK0Z_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7230..7334
                     /locus_tag="ACHK0Z_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /pseudo
     CDS             7230..7334
                     /locus_tag="ACHK0Z_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7497..8207
                     /locus_tag="ACHK0Z_RS19805"
                     /old_locus_tag="pgaptmp_003961"
     CDS             7497..8207
                     /locus_tag="ACHK0Z_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4503900.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            8249..10900
                     /locus_tag="ACHK0Z_RS19810"
                     /old_locus_tag="pgaptmp_003962"
     CDS             8249..10900
                     /locus_tag="ACHK0Z_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4503901.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPELWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSNSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIALGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            10914..11444
                     /gene="ydeS"
                     /locus_tag="ACHK0Z_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             10914..11444
                     /gene="ydeS"
                     /locus_tag="ACHK0Z_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4503902.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
BASE COUNT         3194 a         2495 c         2903 g         2868 t
ORIGIN      
        1 aagaatattc attagtgggc gttcaggacg gttcccacgg tgtcgtcaac gtgaccgata
       61 aagggcattg gaatttcctc ggaacgggcg aagctttccg ctatatctat atcggtgatg
      121 ctggcgacgg tgaacttaat gtctcgcgtg aaggtaaagt agattcggga attatcactg
      181 cggggatgaa agaaacaggc acaggcaaca ttactgttaa ggataagaac tccgttatca
      241 ctaatctcgg aactaatctt ggttatgacg gccacggcga aatgaatatc agtaatgaag
      301 ggcttgttgt cagcaacgga ggaagctcac tcggttatgg agaaaccggc gtcgggaatg
      361 tcagcatcac cacggggggg atgtgggagg tcaataagaa tgtctatacc accattggtg
      421 ttgcgggcgt cggaaacctc aatattagcg atggcggtaa gttcgtatcg caaaatatta
      481 cttttttggg cgataaagca agcggtatcg gcacactgaa cctgatggat gcgacatcat
      541 cgttcgatac tgtgggtatc aatgtcggta attttggtag cggtatcgta aatgtcagta
      601 atggtgccac ccttaattca acgggctatg gatttatcgg aggaaatgcc tccggtaagg
      661 ggatagttaa tatttcaacg gacagtctct ggaatttaaa gacatcatct actaacgcac
      721 aattgctaca ggtcggtgta ttaggcaagg gtgaactgaa tattaccacc ggaggtatag
      781 ttaaagcgcg tgatacacag atagctctca atgacaaaag taagggcgac gtgagggtgg
      841 atgggcagaa ctctcttctt gaaacattca atatgtacgt agggacatct ggtacgggta
      901 cgttaaccct gacgaatagc ggcacgctga atgtcgaagg tggagaagtt tacttaggtg
      961 tttttgaacc tgctgtagga acgctaaaca ttggtgcagc tcacggtgag gcggcggcag
     1021 atgccgggta tataaccaac gcgacgaaag tagagtttgg ttctggcgaa ggtgtttttg
     1081 tctttaatca tactaataac agtgatgccg gctaccaggt cgatatgctg attacaggtg
     1141 acgataaaga cggaaaagtg atccatgatg caggccatac ggtgttcaat gcagggaata
     1201 cttatagcgg taaaacgctg gtcaatgacg gcctcctgac cattgcgtct catacggcag
     1261 atggggtaac gggcatgggt tcgagtgaag tcaccattgc aagccccggt acgctcgaca
     1321 ttctcgcatc aacgaacagt gcaggagatt acacgctgac caatgcgctc aaaggcgatg
     1381 gcttgatgcg agtgcagctg tcatcctacg acaagatgtt tggctttacc catgcaacag
     1441 ggactgaatt cgccggtgtt gcccaactga aagacagtac cttcactctg gaacgcgaca
     1501 acaccgctgc gcttactcac gcgatgttgc agtctgacag tgaaaatacc acatcggtaa
     1561 aagtgggaga gcaatccatt ggtggactgg ccatgaatgg cggtacgctc attttcgata
     1621 cggatattcc tgctgcgacg cttgcagagg gatatatcag cgtcgatacg ctggttgtcg
     1681 gcgcgggtga ctacacctgg aaaggccgta actatcaggt aaacgggacg ggcgacgtgc
     1741 ttatcgacgt gcctaaaccg tggaatgatc ccatggcgaa taaccctctg acgacgctca
     1801 atttgctgga acacgacgat agccatgtcg gcgttcaact ggtgaaggcg caaacggtta
     1861 ttgggtcggg tggctcatta acgttacgtg atttacaggg cgacgaggtg gaagcggaca
     1921 aaacgttaca cattgcgcaa aacggaacgg tggtcgccga gggtgattat ggattccgcc
     1981 tcacgaccgc accaggtgat ggtttgtacg ttaactatgg gctgaaagcg ctgaacatcc
     2041 atggtgggca aaagctgacg ttagccgaac atggcggagc ctatggcgca acggccgata
     2101 tgtcggcaaa aatcggtggt gaaggggatc tggcaatcaa tacggtgcga caggtttcgc
     2161 tttccaacgg tcagaacgac tatcaggggg caacctacgt tcagatgggg acattacgta
     2221 ccgatgcgga tggtgcactt ggcaacaccc gggaactgaa catcagcaac gcggccatcg
     2281 tcgatcttaa tggatcgacg cagacggtag agacattcac cgggcagatg ggttcgactg
     2341 ttttgttcaa agagggggcg ctgacggtaa ataaaggtgg gatcagtcag ggtgaactga
     2401 caggtggcgg aaacctgaat gttacagggg gaacgctggc tatcgagggg cttaatgcac
     2461 gctacaatgc gttaaccagc attagcccaa atgcggaagt cagcctcgat aatactcagg
     2521 ggttaggcag aggaaatatt gccaatgacg gtctgttaac gctaaaaaac gtgactggcg
     2581 aactgcgtaa tagcataagc gggaagggta tcgtgagcgc aaccgccagg acagatgtag
     2641 agctggatgg cgataatagc cgctttgtgg ggcaattcaa cattgataca ggcagcgcgc
     2701 tcagcgtcaa cgagcagaaa aacctgggtg atgcttccgt tatcaataat ggcctgctca
     2761 ccatctccac tgagcgtagc tgggcgatga cgcacagtat cagcggtagc ggtgatgtga
     2821 caaaactggg taccgggatc ctgactctta acaacgattc cgcggcgtat cagggtacga
     2881 cggatatcgt ggggggggaa attgctttcg gttccgactc tgccattaat atggcaagtc
     2941 aacacattaa tatccataac agcggtgtga tgtcgggaaa tgtcaccact gcaggtgatg
     3001 tgaacgttat gcctgggggg acactgcgtg tcgctaaaac cacaatcggc ggcaacctgg
     3061 agaatggcgg cacggttcaa atgaatagcg aaggggggaa accggggaat gtactgaccg
     3121 ttaacggcaa ctataccgga aacaatggcc tgatgacgtt caacgcgacg ctgggcggcg
     3181 ataattcgcc caccgataaa atgaacgtga aaggcgatac ccaagggaac actcgcgttc
     3241 gggttgataa cattggcggc gtcggtgcac aaacggtcaa cggtattgaa ctcattgagg
     3301 ttggcggtaa ttctgcaggt aacttcgcgc tgaccaccgg aactgtcgaa gctggggctt
     3361 acgtctacac gctggctaaa gggaagggga atgacgagaa aaactggtat ctgaccagta
     3421 aatgggacgg cgtaacgcca ccggatacgc ccgaccccat caataatccc cctgttgtgg
     3481 atccggaagg cccatcagtt tatcgcccgg aggccggaag ctatatcagc aacattgccg
     3541 cagccaactc gctgtttagc catcgtttac acgaccgtct gggtgagccg cagtatatag
     3601 attcactgca ttctcagggg tcggcaagca gtatgtggat gcgtcatgtc ggagggcacg
     3661 aacgttcaag ggccggtgac ggtcagctaa atactcaggc taaccgctat gtattgcagc
     3721 taggcggcga tttggcgcag tggagtagca acgcgcagga tcgctggcat cttggcgtga
     3781 tggcaggtta cgctaatcag cacagtaata ctcagagtaa tcgtgtgggt tataaatcgg
     3841 atgggcgcat cagcggttac agcgctgggc tgtacgcgac ctggtatcag aacgatgcga
     3901 ataagaccgg cgcttatgtt gacagctggg cgctgtataa ctggtttgat aacagcgtca
     3961 gttccgataa ccgttctgct gacgactatg attctcgcgg tgtgacggcc tctgttgagg
     4021 gtgggtatac ctttgaagcg ggaacattta gcggcagcga agagacgctg aatacctggt
     4081 acgtccagcc acaggcgcaa atcacctgga tgggtgtgaa agattccgac catacccgga
     4141 aagacggaac gcgcattgaa acggaaggcg acggaaatgt gcaaacgcga cttggggtga
     4201 aaacctacct gaacagccat caccagcgtg acgatggtaa acagcgtgag ttccagcctt
     4261 acattgaagc gaattggatc aacaacagca aagtctacgc cgtgaagatg aatggtcaaa
     4321 ccgtaggccg tgaaggtgcg cgtaatctcg gtgaagtacg taccggggtt gaggcgaaag
     4381 taaataacaa ccttagcctg tgggggaatg tcggtgtgca actaggtgat aaaggctata
     4441 gcgatactca gggcatgctg ggagtgaaat atagctggta aaccgtataa gccgcatgtc
     4501 gagatggcat gcggcttaat attgccgact tcaaaggctt taaagcctcc cttttatccg
     4561 cgatcgcgga tatcgcagcg tttatcccgt acagcggata agatgtgttt ccagattgac
     4621 tttatcctca ctaaaggata aaactgataa tatcccctta agcggataaa tttgctgtgg
     4681 acgtatgaca tgatgagctt tcagaagatc tatagcccaa cgcaattggc gaatgcaatg
     4741 aaactggttc gccagcaaaa tggctggacg cagagcgagc tggcgaaaaa aattggcatt
     4801 aagcaggcga caatttccaa tttcgaaaac aaccctgaca ataccacgct cacgacattt
     4861 tttaagattt tacagtcgct tgaactctca atgacactat gcgacacgaa aaatgcctcg
     4921 ccagaatcaa cagaacagca agatctggag tggtaatgcc taaacttgtc acttggatga
     4981 acaaccagcg ggtaggcgag ttaacgaagt tagccaacgg cgcgcacacc tttaagtatg
     5041 caccggagtg gttagccagc cgttatgcca gaccgttgtc actttcgctg ccattgcaga
     5101 gggggaatat cacctcagat gccgtattta acttcttcga taacctgtta cccgatagcc
     5161 cgattgtacg tgaccggatc gttaaacgtt atcatgctaa atccagacaa ccgtttgatt
     5221 tattgtcaga aatagggcga gacagcgttg gtgccgtaac gttattaccc gaaaatgaaa
     5281 ccataacgcg cccgataatg gcatgggaaa agcttactga agccaggctt gaagaagtat
     5341 taacggctta taaagcagat atccctctgg gcatgattag agaagaaaat gactttcgca
     5401 tctcggttgc tggcgcgcag gagaagacag cgctgcttag aataggcaat gactggtgca
     5461 ttccgaaagg aataacgccg acgacgcaca tcattaaatt accgattggc gaaatcaggc
     5521 agcccaatgc gacgctcgat ctcagccaaa gcgttgataa tgagtattac tgtctgctgc
     5581 tggcgaaaga acttgggttg aatgttccgg acgcagaaat cattaaagcg ggaagggtgc
     5641 gcgcgttagc ggtcgaacgt tttgacaggc gttggaatac tgagcgaacg gttttacttc
     5701 gcttgccaca ggaggatatg tgtcagacat tcggtttacc ttcatctgtg aaatatgaat
     5761 cagatggagg cccaggcatc gcgcggatca tggcgttttt gatggggtcc agcgaggcgc
     5821 ttcgcgatcg ttatgatttt atgaaattcc aggtcttcca gtggttgatt ggcgcaacgg
     5881 atggccatgc aaaaaacttc tccgtattta ttcaggctgg cggcagttat cgactcacgc
     5941 cattttacga catcatttca gcatttccgg tccttggcgg tacgggaata cacatcagcg
     6001 atctcaaact ggcaatgggg cttaacgcat ccaaaggcaa aaaaacggca atcgataaaa
     6061 tttatccgcg acattttttg gcgacagcaa aggtgctgag attcccggaa gtgcagatgc
     6121 atgaaatcct aagtgacttt gccagaatga ttccggcagc actggataac gtgaagactt
     6181 cattaccgac agattttccg gagaacgtgg tgacggcagt tgaaaccaat gtgttgaggt
     6241 tgcatggacg gttaagccga gaatacggta gtaagtgaga tatggggttt ggtcgttgtt
     6301 aatgagcacg acaatcatga ccgccaacga ctggattctt ccttggttta tactggtgcg
     6361 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     6421 tattcgttca gggttcaaga acttaagcct ctcctctttc ataattggcc tgtgcatgga
     6481 gaaatagcct ggttattccc tgccccatgc taagagcgca gtgtctgtcg catagacact
     6541 gcatacgaaa acaccagagg gtgctgttac ttatttaagg acggatcaag cgggaatgag
     6601 tccactccgg tatcagaaat ggctacgtct caatgaagtc aggcgaccga tgctgaatga
     6661 acattacgat gtcaccacag cagcctatgc tgtcggttac gaaagctatc ccatttcagt
     6721 cgggaatatt cgcggatgtt tggagagtca cccaagagag atattaccag gttaagaaag
     6781 tctgtcggtc agctttaata aaccctgcac ttatctgttt agagcaattc ggtgttagtt
     6841 tcagcaagca aacattaacc atagctaatg atttatagcc atattaacca ttggtagccc
     6901 ttgacctttc ctgacacttc ccttgttcgc aataatattc tgtttagtca cggtttataa
     6961 ataacatgca taatgaaatc gtcatgttat ttagcgacat aaaatgtttt tgcaaatact
     7021 ctcttcaaca agtgcacaat aaacttgttg tgattaggtg tccttgtgat ttaaagatat
     7081 tatcgttctt cgtgaaatgt aataaccctg gaaactatat tctcagggca acaaccaatc
     7141 aagaatgatt cttgttttta agcaataaca gaatgactta attgttaacc ctctttattc
     7201 agctacttaa atataaattt tggagaatta tgaaactcaa acatgttggt atgattgtcg
     7261 tttctgtgtt ggcgatgtcg tctgctgcgg taagcgcagc cgagggtgat gaatcagtaa
     7321 cgaccactgt taatttactt tgtagcaagg ttttcctatt aaaagagaac caacatttat
     7381 tacaaacgca ttaagtctgg aaacccggcg actcgccaat gtattagcaa cattaatcga
     7441 ttgtctgatg taacggtttt cccgcttatc ctcttaacac atggagaatg catcagatgc
     7501 aaactacgcg taccccctac tctatttcct ttatggcgac agtgttactt ttattgctct
     7561 ttgcttgcca tagcaccgtt gccaacgcag ctgtcgcact gggtgcgacg cgggtaattt
     7621 atccggcaaa ccagaaacag gttttattgc ctgtcacgaa taatgatcct gccagtgtct
     7681 atctcattca gtcatggata gaaaatgcag gtgaccaaaa agacacccag tttgtcatta
     7741 ccccaccgct tttttccatg caaggtaaaa aagagaatac gctgcggata atcaacgcta
     7801 ctaatcatca gttaccaggc gaccgtgaaa gcctgttctg ggtgaatgtt aaagctattc
     7861 ctgctatgga aaaagaccag aagaatgaga atacattaca gctggcaatt attagccgca
     7921 taaaaatgtt ttatcgcccc acgaacctgg caatggcacc tgaagaagcg ccagcaatgc
     7981 ttcggtttcg tcgcagcggt agcaaactca ctcttattaa tccaacgccc tacttcatta
     8041 ccgtaacaaa tatgaaagca ggaaatagca atctcccaaa caccatggtg ccgccaaaag
     8101 gtgaagtatc ggtggatatc ccgcatgctg caaccgggga tatcagtttt cagacgatca
     8161 atgattacgg tgcattaact ccgcgtatta aggcaaccat gcagtaagcc agtgacttaa
     8221 ttaataataa actcaatcgg aattaataat gacagccttt cgtgccgcat ttaaggcata
     8281 tcgtatgcat caggtgttaa tactaccacg ctttgcacgg cttaccattg cccttggctt
     8341 agcaactgcg gtttttcccg ttgatgcaga gtattatttt aatccacggt ttttaagcaa
     8401 tgatcttgct gaatctgtcg atttatccgc ctttactaag ggacgcgagg caccacctgg
     8461 tacatatcgg gtcgacattt atttgaacga cgaatttatg actagtcggg atattacttt
     8521 tattgcagat gataataacg cagatctcat cccatgtctg agtacagacc ttcttgtcag
     8581 tcttggcata aaaaaatctg cgttattaga caataaggaa cattctgcag aaaaacatgt
     8641 gccggacaac agcgcatgca caccacttca ggatcgtttg gccgacgcat caactgagtt
     8701 tgatgttggt cagcaacatt tgtcactctc cgttccgcaa atttatgttg gcagaatggc
     8761 tcgcggctat gtttctcctg aactgtggga agaaggaata aatgctgggc ttctaaatta
     8821 tagttttaat ggtaattcca ttaataatcg tagtaaccat aatgcaggaa aatccaacta
     8881 tgcatatttg aatttacaga gtggcatcaa cattggtagt tggcgactgc gcgataactc
     8941 aacgtggagt tataacagtg ggagtagcaa ttcatctaac agcaataaat ggcagcatat
     9001 caatacgtcg gctgaacgtg acattattcc cttacgctca cggttaacgg taggtgatag
     9061 ttataccgat ggcgatattt ttgatagtgt gaactttcgt ggcctcaaaa taaattcaac
     9121 agaagcgatg ttgcccgata gccaacatgg tttcgctccg gtgattcatg gtattgccct
     9181 tggcaccgca caagtgagtg taaaacagaa cggatacgat gtttatcaga ctactgttcc
     9241 acccggccct tttactattg atgatatcaa ctctgccgcc aatggtggtg atctgcaagt
     9301 aaccataaaa gaggcagacg gcagtattca gacattatat gttccttatt cgtctgttcc
     9361 ggttctccaa cgtgctggat atacgcgtta tgcgcttgcc atgggggaat atcgtagtgg
     9421 aaataacctg caaagctccc ccaagttcat acaaggtagc ttgatgcatg gactggaagg
     9481 aaactggaca ccttatggcg gaatgcaaat tgcagaagat tatcaggcct tcaaccttgg
     9541 tattggtaaa gatttaggac tttttggtgc cttttctttc gatatcacgc aggccaatac
     9601 gacacttgca gatggcaccc gtcacagcgg gcaatcggtt aaatccgtct acagcaaatc
     9661 cttctaccag acaggaacca atatccaggt cgcaggatat cgctattcta cgcaaggttt
     9721 ttataactta tccgacagtg cctacagtcg aatgagtggt tacaccgtca agcctcctac
     9781 cggagacacc aatgagcaga cacaatttat tgattatttt aatctgttct acagtaagcg
     9841 tggtcaggaa caaataagca tctctcagca gcttggaaat tacggtacga catttttcag
     9901 tgccagtcgc caaagttact ggaacacgtc acgcagcgac cagcaaatat catttggatt
     9961 aaatgtgccg tttggtgata ttacgacttc gctgaattac agctattcca ataatatatg
    10021 gcaaaacgat cgggatcatt tactcgcttt tacgcttaat gttcccttca gtcattggat
    10081 gcgtacagac agtcagtcgg catttcgtaa ttcaaacgcc agttacagta tgtcaaacga
    10141 tttgaaaggc ggcatgacca atctatcggg ggtttatggc actctgctgc cggataataa
    10201 cctgaattat agcgttcagg tcggtaacac ccacggaggt aatacatcgt ctggcaccag
    10261 tggttacagt tctcttaatt atcgtggagc ttacggtaat acgaatgtcg gttacagtcg
    10321 gaatggtgac agcagccaga tttattacgg aatgagtggt gggattattg ctcatgctga
    10381 tggcatcacc tttggacagc cgctgggcga cacaatggtt ctggttaagg ctcctggcgc
    10441 tgataatgtc aaaatagaga accagaccgg aattcatacc gactggcgtg gctatgccat
    10501 attaccattt gcgacagaat atagagaaaa ccgtgttgct cttaacgcga attcccttgc
    10561 agataatgtt gaactggatg aaaccgtggt cactgtcatc ccaactcacg gtgctattgc
    10621 cagagcaaca tttaatgcac aaatcggcgg gaaagtatta atgacgttga agtacggtaa
    10681 taagagcgtt ccattcggtg caattgtcac acacggagag aataaaaatg gcagcattgt
    10741 cgcagaaaac ggtcaggttt atctgactgg acttccacag tcagggaaat tacaggtttc
    10801 atggggcaaa gataaaaact caaactgtat tgtcgagtac aagcttcctg aagtttctcc
    10861 tggtacctta ctgaaccagc agacagcaat ctgtcgctaa ggaacaatag attatgaaat
    10921 acaataacat tattttcctc ggtttatgtc tggggttaac cacctattct gctttatccg
    10981 cagatagcgt tattaaaatt agcgggcgcg tcctcgatta tggctgcaca gtctcatcgg
    11041 attcgcttaa ttttaccgta gatctccaaa aaaacagtgc cagacaattt ccaacgaccg
    11101 gtagcacaag tccagtcgtc ccttttcaga ttacgttaag tgaatgcagc aaagggacaa
    11161 cgggggttcg ggttgcattt aacggtattg aggatgcaga aaataatact ttgttgaaac
    11221 tggatgaagg aagcaatacg gcctccggtt tgggtataga aatattggac gcaaatatgc
    11281 gtccggtgaa actgaatgac cttcatgccg ggatgcagtg gatcccactg gtaccagaac
    11341 agaacaatat tttgccttac tccgctcgtc tgaagtcaac tcagaagtcc gtcaatccgg
    11401 gactggtgag ggcttcggca acctttaccc ttgaatttca ataatcagag tcagatatga
//
LOCUS       DAZFWP020000151        11418 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFWP020000151 DAZFWP020000000
VERSION     DAZFWP020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.151"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            297..878
                     /gene="mdaB"
                     /locus_tag="ACHK0Z_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             297..878
                     /gene="mdaB"
                     /locus_tag="ACHK0Z_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU4503903.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            909..1223
                     /locus_tag="ACHK0Z_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             909..1223
                     /locus_tag="ACHK0Z_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU4503904.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1271..3163)
                     /gene="parE"
                     /locus_tag="ACHK0Z_RS19830"
                     /old_locus_tag="pgaptmp_003966"
     CDS             complement(1271..3163)
                     /gene="parE"
                     /locus_tag="ACHK0Z_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU4503905.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDRDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3192..3773)
                     /gene="yqiA"
                     /locus_tag="ACHK0Z_RS19835"
                     /old_locus_tag="pgaptmp_003967"
     CDS             complement(3192..3773)
                     /gene="yqiA"
                     /locus_tag="ACHK0Z_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU4503906.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3773..4600)
                     /gene="cpdA"
                     /locus_tag="ACHK0Z_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             complement(3773..4600)
                     /gene="cpdA"
                     /locus_tag="ACHK0Z_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU4503907.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4625..5047)
                     /locus_tag="ACHK0Z_RS19845"
                     /old_locus_tag="pgaptmp_003969"
     CDS             complement(4625..5047)
                     /locus_tag="ACHK0Z_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU4503908.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5048..5677)
                     /gene="nudF"
                     /locus_tag="ACHK0Z_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             complement(5048..5677)
                     /gene="nudF"
                     /locus_tag="ACHK0Z_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU4503909.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5882..7363
                     /gene="tolC"
                     /locus_tag="ACHK0Z_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             5882..7363
                     /gene="tolC"
                     /locus_tag="ACHK0Z_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU4503910.1"
                     /translation="MKKLLPILIGLSLYGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7511..8182
                     /locus_tag="ACHK0Z_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             7511..8182
                     /locus_tag="ACHK0Z_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU4503911.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8188..9348
                     /locus_tag="ACHK0Z_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             8188..9348
                     /locus_tag="ACHK0Z_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4503912.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQIEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9386..10174)
                     /gene="ygiD"
                     /locus_tag="ACHK0Z_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             complement(9386..10174)
                     /gene="ygiD"
                     /locus_tag="ACHK0Z_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU4503913.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10317..11090
                     /gene="zupT"
                     /locus_tag="ACHK0Z_RS19875"
                     /old_locus_tag="pgaptmp_003975"
     CDS             10317..11090
                     /gene="zupT"
                     /locus_tag="ACHK0Z_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU4503914.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11148..11318)
                     /gene="yqiD"
                     /locus_tag="ACHK0Z_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             complement(11148..11318)
                     /gene="yqiD"
                     /locus_tag="ACHK0Z_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU4503915.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         2840 a         3070 c         2816 g         2692 t
ORIGIN      
        1 ccatttataa ttatcaacat gacacgttga tattttcatc ttttacttat tcaaagccat
       61 attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat
      121 tatgaggaat ggctatcagt attgtcattt tcagaaaata tttatcctgc atcggtgagt
      181 cagagcaaga tcagactttt gctaaattcg caaaagactt tgcacatttt gctaatttca
      241 ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt taaaaaatga
      301 gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg
      361 acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcatcg
      421 ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc tgggctgatg
      481 tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata
      541 ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc cagcgatggc cgtacccgca
      601 aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt
      661 ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc ttccacggcg
      721 ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt ggcatggaac
      781 cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg
      841 aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg ctttagaagg
      901 agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc
      961 aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc
     1021 acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg
     1081 attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa
     1141 ccccgcacat gaaggcgtac agcgaagccg taaaaggcga cgtgctggag atgaatatcc
     1201 gcatcctgca acctgggatt taaacctgcc ttgtttgccc ggccatcctg accgggcaat
     1261 gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac cagttgcggc
     1321 gatcttccga gcgtttcttc gccagcagca tatccatcat cgcgtcagta cgctgatcgt
     1381 cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac
     1441 gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc ggcttgcctt
     1501 tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc gcgtaataaa
     1561 cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt
     1621 tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag cagcgtggca atgtgcagac
     1681 catcagagtc cgcatccgcg aggatacaga ttttgccata acgaagctgg ctcagatcgt
     1741 cgcggtcagg atcgataccg atcgctaccg aaatatcatg cacttcctgc gaggccagca
     1801 cttcgtcgga agaaacttcc caggtgttaa ggatcttacc tttcagtggc atgatcgcct
     1861 gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct tccacaagga
     1921 acagttcggt acggttaaga tcctgcgcgg tacagtcagc cagtttgcca ggcagtgctg
     1981 ggccgctggt cagctttttg cgcaccactt ttttagccgc acgcatacgg cgctgggcgc
     2041 tggaaatcgc catctccgcc agcagctccg ccgcctgaac gttctggttc agccacagga
     2101 taaaggcatc tttcaccacg cctgaaacga atgccgcgca ttgacgcgaa gagagacgct
     2161 ctttggtctg cccggcaaac tgcggatcct gcattttcac cgacagaaca taggcgcagc
     2221 gatcccagat atcttccgcc gacagcttca cgccgcgcgg cagaatgttg cggtattcac
     2281 agaactcacg catcgcgtcc aacaggccct gacgcagacc attaacatgg gtaccgccct
     2341 gcatcgttgg gataaggttg acgtagcttt cggtcagcag ttcaccgcct tccggcagcc
     2401 acagtagcgc ccagtccaca gcttcagtat cgccagcgaa attaccgata aacggttttt
     2461 ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga ccgtcctgat
     2521 agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca acgccggggc
     2581 acagtactgc tttggccttc agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt
     2641 caaagaaggt ttcatccggc cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag
     2701 tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg ttataaacct
     2761 gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc accgaaatcc
     2821 ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta ccgcctgcat
     2881 gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga atatccaccg
     2941 gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaag attacatcca
     3001 cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt atcaataact tcttgcccca
     3061 aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc ggctcaagcc
     3121 cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa gttagcaatt
     3181 cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt gaaataatct
     3241 tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca ggaagcgtag
     3301 tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag cagccagatc
     3361 aaatccggcg cttccagcgg gtcaatctgc atgactttga gatcgtaaat atggcgtgac
     3421 tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata gtccgtcagc
     3481 agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa acattgtgac
     3541 aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga atcgccgcca
     3601 tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg cggcaactgc
     3661 ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa caagctcgct
     3721 tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc
     3781 cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca
     3841 gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatagta tccagcgtaa
     3901 agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc aggcggcgac
     3961 cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg
     4021 gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc
     4081 aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg cgttctggcg
     4141 catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca ccgtgcggca
     4201 cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata aacacgcgct
     4261 tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt
     4321 tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat
     4381 aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt
     4441 gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg tttaccccta
     4501 acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa atcctgactc
     4561 tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct taggtttcac
     4621 gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc aaccagtccg
     4681 ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta ttaggataat
     4741 cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc accatcgcgt
     4801 catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg ggcgcagtct
     4861 gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc cgatattgtg
     4921 cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc
     4981 gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac
     5041 gcttcattta tgcccactca ttttttaacg cttgatgatg cagctgcagc cattgcaaag
     5101 cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat gcctgttccc
     5161 ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga ataccgcttg
     5221 cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg ccccccgggc
     5281 ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg
     5341 cttcgcgacg ggcaacatct tccacacttt caccctcttc tatcatcccg gcaaccatct
     5401 ccagtagcca gggggtttca ctggtgtcgt atgcggcaat ccgaatctgc tcaatcagca
     5461 caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa
     5521 tttccctccg tacctcatga ctcatttgcc cgttgaataa acgatgacga aatctataaa
     5581 gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct acatcgtttt
     5641 tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg gcactaatag
     5701 tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat gtgttaaatt
     5761 gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta
     5821 gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac aaggaatgca
     5881 aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tatgggttca gttcgttgag
     5941 ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc cggaattgcg
     6001 taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt
     6061 actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc gcgacgcgaa
     6121 cggcatcaac tctaacgcga ccagtgcgtc cctgcagtta actcaatcca tttttgatat
     6181 gtcgaaatgg cgtgcgttaa cgctgcagga aaaagctgca gggattcagg acgtcacata
     6241 tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca acgtgttgaa
     6301 tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc gtcaattaga
     6361 tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc agaacgcccg
     6421 cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc ttgataacgc
     6481 ggtagagcag ctgcgccaga tcaccggtaa ctactatcca gaactggcag cgctgaatgt
     6541 cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa
     6601 acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg agcaaattcg
     6661 ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg ggatttctga
     6721 cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg acgacagcaa
     6781 tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg gcggaatggt
     6841 taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc aactggaaag
     6901 cgcgcatcgt agcgtcgtgc aaaccgtacg ttcctccttc aacaacatta atgcatctat
     6961 cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat tagacgcgat
     7021 ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg caaccaccac
     7081 gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga ttaatcagct
     7141 gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac tgaacaatgc
     7201 gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa
     7261 tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc aaacatccgc
     7321 acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac gacggggctt
     7381 cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca ttcttccccc
     7441 ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc actggtcccg
     7501 gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca aaaactggag
     7561 cgcgcgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc tggctggctg
     7621 tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa
     7681 cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg
     7741 tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg
     7801 ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg cccagcaatc
     7861 cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc tgatgggcgg
     7921 cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca gcccggctta
     7981 cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag gccgcaccat
     8041 gaccgtaccg aagacagcca tggcaccaaa accggcgacc accactaccg ttacccgtgg
     8101 cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa ctggcacctc
     8161 ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga gcgcccggac
     8221 tggcgtgaga aagcccacga atacggtttc aattttcaca ccatgtacgg cgagccgtac
     8281 tggtgtgaag atgcttacta caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc
     8341 accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag
     8401 ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgcca gtcatggctg
     8461 acgcaccagc catcgcttta ttcgcgtctt gatctggcgt gggatggcac tggcgaacct
     8521 aaacttctgg aaaataacgc cgatacgcca acgtcactat acgaggcggc gttctttcag
     8581 tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag cgaccagttt
     8641 aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc
     8701 cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac cattcagtat
     8761 ttgcaggact gcgcaacgga agctgaaatt gctactgagt tcctctacat cgatgatatc
     8821 gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc caacctgttc
     8881 aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt ccaccaagct ggaggatgca
     8941 ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaggc acttctaccg
     9001 ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt tgcggaagat
     9061 gatcatccgc aaatagaaaa atatgtggtt aaaccgatct tctcccgtga aggcgcaaac
     9121 gtgtcgatca ttgaaaacgg caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa
     9181 gggatgattg ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta tatgctgatt
     9241 ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga ccgtgcattg
     9301 atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc aatgatgccg
     9361 gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata ctcaggctgc
     9421 ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac gcacctaaga
     9481 catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca ccttcatggt
     9541 cgaggtaatt caccagagga tgctgctcta ctgccccttg ccacgtcaga ttcgctttca
     9601 catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc cacttcactg
     9661 tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca tctcgcagcg
     9721 ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca
     9781 actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc caggaaccgt
     9841 ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac cggcgctaac agctcaacca
     9901 gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac agcgcctgcg
     9961 ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac
    10021 gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca
    10081 acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac
    10141 ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca
    10201 ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat
    10261 gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag ttgtcgatgt
    10321 cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg
    10381 gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg
    10441 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
    10501 tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat tttggcctgg
    10561 accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg cagccgttgc
    10621 caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc
    10681 cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg
    10741 cactggccgt cgctttgcac aatatccctg aaggtctggc agtggcaggc ccggtttatg
    10801 cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga ctagcagaaa
    10861 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg gtggtcatgg
    10921 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
    10981 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
    11041 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aaacaacatc
    11101 agaagttgtc cagcacatgg acaacttctg ccagcttcac gaaagcctta ttctaccttt
    11161 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
    11221 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaacc
    11281 agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac
    11341 aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc
    11401 gcataataac ggaacaat
//
LOCUS       DAZFWP020000152        11326 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFWP020000152 DAZFWP020000000
VERSION     DAZFWP020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.152"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            213..1784
                     /gene="garD"
                     /locus_tag="ACHK0Z_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             213..1784
                     /gene="garD"
                     /locus_tag="ACHK0Z_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU4503916.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGMAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1933..2268
                     /gene="prlF"
                     /locus_tag="ACHK0Z_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             1933..2268
                     /gene="prlF"
                     /locus_tag="ACHK0Z_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU4503917.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2268..2732
                     /gene="yhaV"
                     /locus_tag="ACHK0Z_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             2268..2732
                     /gene="yhaV"
                     /locus_tag="ACHK0Z_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU4503918.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2787..3596)
                     /gene="agaR"
                     /locus_tag="ACHK0Z_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(2787..3596)
                     /gene="agaR"
                     /locus_tag="ACHK0Z_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU4503919.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3845..5125
                     /gene="kbaZ"
                     /locus_tag="ACHK0Z_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             3845..5125
                     /gene="kbaZ"
                     /locus_tag="ACHK0Z_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU4503920.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYTSANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARAMLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5148..5621
                     /gene="agaV"
                     /locus_tag="ACHK0Z_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             5148..5621
                     /gene="agaV"
                     /locus_tag="ACHK0Z_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU4503921.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5632..6411
                     /gene="agaW"
                     /locus_tag="ACHK0Z_RS19915"
                     /old_locus_tag="pgaptmp_003983"
     CDS             5632..6411
                     /gene="agaW"
                     /locus_tag="ACHK0Z_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU4503922.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGIAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6401..7279
                     /gene="agaE"
                     /locus_tag="ACHK0Z_RS19920"
                     /old_locus_tag="pgaptmp_003984"
     CDS             6401..7279
                     /gene="agaE"
                     /locus_tag="ACHK0Z_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU4503923.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7297..7731
                     /gene="agaF"
                     /locus_tag="ACHK0Z_RS19925"
                     /old_locus_tag="pgaptmp_003985"
     CDS             7297..7731
                     /gene="agaF"
                     /locus_tag="ACHK0Z_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU4503924.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7728..8861
                     /gene="nagA"
                     /locus_tag="ACHK0Z_RS19930"
                     /old_locus_tag="pgaptmp_003986"
     CDS             7728..8861
                     /gene="nagA"
                     /locus_tag="ACHK0Z_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4503925.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAQPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGNTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9212..10366
                     /locus_tag="ACHK0Z_RS19935"
                     /old_locus_tag="pgaptmp_003987"
     CDS             9212..10366
                     /locus_tag="ACHK0Z_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU4503926.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10379..11239
                     /gene="kbaY"
                     /locus_tag="ACHK0Z_RS19940"
                     /old_locus_tag="pgaptmp_003988"
     CDS             10379..11239
                     /gene="kbaY"
                     /locus_tag="ACHK0Z_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU4503927.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2721 a         2924 c         2989 g         2692 t
ORIGIN      
        1 attattttga gcatatgcac ataaggttgc gcgctaaagc acagatttgc gctttacctt
       61 gccgggcggc actgcaatcc ctgaaatgat tgacattgat cacatttctg cgtttaaact
      121 cctgacattc ttatttcacc caatgaagtc atttattttt aaatgagacc aggtcctcat
      181 tttaataacc cctggctgga gaatatagta caatggccaa catcgaaatc agacaagaaa
      241 cgccaactgc gttttatata aaagttcacg acacagataa tgtggcaatt attgttaatg
      301 ataatggcct gaaagcagga acgcgttttc cggatggact ggaattaatt gaacatattc
      361 cccaggggca taaagtcgca ttgctggaca ttccggctaa tggtgaaatt attcgttatg
      421 gtgaagtgat tggttacgcc gtgcgtgcaa tcccacgcgg aagctggatc gacgaatcaa
      481 tggttgtact accggaagcg ccgccgttac acacgctgcc actggcaacc aaagtcccgg
      541 cccccttacc gccgctggaa ggatacacct ttgagggcta tcgcaatgcc gatggcagcg
      601 tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca ctgtgtggca ggcgtggtgg
      661 actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa atacccgaac gtcgatggcg
      721 tggtggggct gaatcatttg tacggctgtg gcatggcgat taacgcaccg gcggcagttg
      781 tgcctattcg taccatccac aatatttcgc tgaaccctaa ctttggcggc gaagtaatgg
      841 tgattggtct gggttgtgaa aagttgcagc ctgagcgcct gctggtcggc acggatgatg
      901 tgcaagctat tccagtagaa agcgccagca ttgtcagttt gcaggatgaa aagcatgtcg
      961 gttttcagtc catggtcgag gatattttgc aggtcgccga acgccatcta caaaaactga
     1021 atcaacgtca gcgagaaacc tgtccggctt cagaactggt tgtcggcatg cagtgcggtg
     1081 gcagcgatgc cttttctggc gtaacggcaa acccggcggt tggctatgcg tctgatctac
     1141 tggtgcgctg cggcgcaacg gtgatgttct cagaagtcac ggaagtgcgt gacgcgatcc
     1201 atctgctgac accacgcgca gtgaacgaag aggtcggcaa acggctgctg gaggagatgg
     1261 agtggtacga taactatctc aatatgggaa aaaccgaccg cagcgccaac ccttcgccgg
     1321 gcaacaagaa aggcggtctg gcaaacgtgg tagagaaggc actcggctcc attgctaaat
     1381 cgggtaaaag cgcaattgtt gaagtgctgt cgcccggtca acgcccgact aaacgcggat
     1441 taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg cacgcaacag gtggcttcgg
     1501 gtatcaccgt gcaagtgttt acgaccggtc gtggtacgcc gtacggcctg atggcggtac
     1561 ccgtcattaa aatggcgacc cgcaccgagc tggcgaaccg ctggtttgat ttaatggata
     1621 ttaacgcggg cactatcgcc accggcgaag aaaccattga agaggtgggc tggaagttgt
     1681 tccactttat tctcgacgtc gccagcggga agaagaaaac cttctcggat caatgggggc
     1741 tgcataacca gctggcagtg tttaacccgg caccggtgac ctgatttctt ttggaacgtc
     1801 cctcgcaaaa catggcctta gtgccatgtt tttattgttt aaagccccca cgtccattaa
     1861 taatgcattt gcattacctt agttcaagct tataatttga gcagaaaaca ggctgtaaaa
     1921 ggacagtgaa tcatgcccgc taatgctcgc tctcacgctg tactgaccac tgaatcaaag
     1981 gtcacgatac gcggacaaac aactatcccc gcgccagtgc gtgaggcctt aaaactgaag
     2041 ccaggccagg acagcattca ttacgaaatt ctgcctggtg ggcaagtctt tatgtgccga
     2101 ctgggagatg aacaggagga tcatactatg aatgcatttt tgcgttttct ggatgcagat
     2161 atccagaaca acccgcaaaa aactcgtcca ttcaacattc aacaaggaaa gaaacttgtc
     2221 gctggcatgg acgtcaacat tgatgatgag attggcgacg acgaataatg gattttccac
     2281 aaagggttaa tggttgggcg ctatatgctc atccctgttt tcaggaaacc tacgacgctt
     2341 tagttgccga agtcgagaca ttaaagggaa aagatcctga aaattatcag agaaaagccg
     2401 ccacaaagtt attggcggta gtccataaag tgattgagga gcatatcacg gtcaatccat
     2461 catcaccggc attccgtcat ggcaagtcgt taggctctgg gaaaaataaa gactggtcac
     2521 gggtaaaatt tggtgctggt cgttatcgtc tcttctttcg ttatagtgaa aaagagaaag
     2581 tcatcattct gggatggatg aacgatgaaa acactctgcg cacctacggt aaaaaaacag
     2641 atgcctatac cgtattcagc aaaatgttaa aaagaggaca tcctcctgcc gactgggaaa
     2701 gcctcaccca agagacagaa gaaaaccatt gatggtgttc acaatgcccg ccgaacgtat
     2761 tcgtgttgac cagcgggcac cctttctcac tccccgacca gaatcacttc aaccccagcc
     2821 tttcgcagtc cttccagact atccgcagga atgccttcat caacaatgat catgtcgata
     2881 cgttgagtat caatgatctt atgtaaactg gaacgattga atttactgga atcggtgacc
     2941 acgatgatcc gttccgcaac ttcgcacatc cgacggttta aacgggcttc atcttcatta
     3001 tgtgtgctga cgccgcgctc cagatcgatc gcatctacac caagaaacag catatcgaag
     3061 tggtaatttt gcagcgattg ctcagcctga tcgccgtaaa aagattgcga ctgacggcgc
     3121 aaatgcccgc cggtcatcag cagctcaacg ccttccgctt ccagcaacgc attagccacg
     3181 ttcataccgt tggtcatcgc aattacgtca gtgtgcttac gcatcagacg agcaatctca
     3241 aaagtggtag tcccggaatc gaggatcacc cgatgaccag gctgaatcaa ctcaacggca
     3301 gctttcgcaa cgctgcgttt catcgcggtg ttcagcgcgc ttttatcttc cactgatggc
     3361 tcgactgacg gcgtcgtgct atcgcagatc aacgcgccac cataggcacg cacagcgatc
     3421 ccctgctttt ccagaaacgc cagatcattg cggatcgtca cagtagatac gccatacaat
     3481 gccgacagat cgttaacctg cacactccct tgctgtcgca gacgctgaat gatctgttct
     3541 cgtcgctcgc tggtgccagt cactcgcttc tcacctgaag cgtcggtatt actcatagta
     3601 agtcctttcg taaaactttc gtttcatttc gttttgccta ttaacgcctt tctattaagc
     3661 aaatgcaagc ccaccttgcc cattgacgca agctactctc gtttcagtga ctttcattat
     3721 gtttcttttg tgaatcagat cagaaaacta ttatctttcg ttttattttt atctcaccat
     3781 gacgcagtat caactgaaac aaaacgaaag attaatatcg cagtaatctg aactggagag
     3841 gaaagtgaaa catctgacag aaatggtgag acagcacaaa gcgggcaaaa caaatggaat
     3901 ttatgccgtt tgttccgcac atccgctggt gctggaggct gcaatccgct acaccagtgc
     3961 aaaccaaacg ccgttactga ttgaagcaac ctccaatcag gtagaccagt tcggcggtta
     4021 taccggaatg acgcccgccg attttcgcgg ctttgtttgt cagctcgccg actcgttgaa
     4081 tttcccgcag gatgcgttga ttctgggtgg tgaccatctg gggccaaacc gctggcaaaa
     4141 cctgccggcc gctcaggcaa tggccaatgc cgatgatttg attaaaagct acgttgcggc
     4201 aggattcaaa aaaatccacc ttgattgcag catgtcctgt caggacgatc cgattccctt
     4261 aactgatgac atcgtggctg aacgcgccgc ccgtctggcg aaagtggcgg aagaaacctg
     4321 tcttgaacac tttggcgaag ccgatctgga gtatgtcatt ggtaccgaag tgccggtacc
     4381 tggcggcgcg catgaaacct taagcgagct ggcggtcacc acgccggatg ccgcccgcgc
     4441 catgctggaa gcccatcgtc acgcctttga aaagcaaggt ttgaatgcca tctggccacg
     4501 catcattgcc ctggtggttc aacccggcgt cgaattcgat cacaccaacg ttattgatta
     4561 tcagcccgcc aaagcgagcg ccttaagcca gatggtcgaa aactacgaaa cgctgatttt
     4621 cgaagcgcac tctaccgatt atcaaacgcc gcaatcgctg cgccagctgg tgattgacca
     4681 ctttgccatt ctgaaagttg gcccagcgct gaccttcgcc ctgcgtgaag ctctgttctc
     4741 tctggcggcg attgaagaag aactggtgcc agcgaaagcc tgttctggtc tgcgtcaggt
     4801 gctggaagac gtgatgctcg accgcccgga atactggcaa agccactacc acggtgacgg
     4861 caacgcgcgt cgtctggcgc gtggttatag ctactcggat cgcgtgcgct attactggcc
     4921 ggacagccag attgatgacg ctttcgctca tctggtacgt aatctggcgg attcaccaat
     4981 tccgctgccg ctgatcagcc agtatctgcc gctgcagtac gtgaaagttc gctccggcga
     5041 gctgcagcca acgccacggg aactcattat caaccatatt caggacatcc tggcgcagta
     5101 ccacacagcc tgtgaaggcc aataagcaaa acaaagagga acacgctatg ccaaatattg
     5161 ttttaagccg gattgatgaa cgcttgattc acggtcaggt cggcgttcaa tgggtcggat
     5221 ttgcgggggc aaatctggtg ctggtagcca acgatgaggt tgccgaagat ccggtacaac
     5281 aaaacctgat ggaaatggta ctggcagaag ggatcgccgt acgtttctgg acgctgcaaa
     5341 aagttatcga caacattcat cgcgccgccg atcgacagaa aatcctgctg gtttgtaaaa
     5401 cacccgccga tttcctgacg ctggtgaaag gtggcgttcc ggtgaatcgc attaacgttg
     5461 gcaatatgca ctacgccaat ggcaaacaac aaatcgccaa aacggtttct gtggatgcgg
     5521 gcgatatcgc agcatttaac gacctgaaag ccgctggggt ggaatgcttc gttcagggcg
     5581 tcccgacaga gcctgctgtg gacctcttta aattactttg agggattcat catggaaatc
     5641 agcctgttgc aggcatttgc gttgggcatt atcgccttta tcgctggcct ggatatgttt
     5701 aacggcctaa cacatatgca ccgcccggtg gtcctcggcc cgttggtcgg gctggtactt
     5761 ggcgatctgc ataccggaat tttaaccggc ggtacgctgg aactggtgtg gatggggctg
     5821 gccccgctgg cgggcgcaca gccgcctaac gtgattatcg gtactatcgt cggcacggcg
     5881 tttgccatta ctactggcgt gaaacccgat gtcgcggtcg gtatcgccgt acctttcgct
     5941 gtcgcagtac agatggggat taccttcctg ttctcggtga tgtctggcgt gatgtctcgc
     6001 tgcgaccgga tggcggagaa cgccgatact cgcggcattg aacgcgtgaa ctatctggca
     6061 ctgctggcac tcggcacttt ctattttctc tgcgctttcc tgcctatcta cttcggcgcg
     6121 gaacatgcaa aaaccatcat tgatgtcctg ccgcaacgat taatcgacgg cctcggcgtc
     6181 gcgggcggca tcatgccagc aatcggtttt gccgtgctgc tgaaaatcat gatgaaaaac
     6241 gtctacatcc cctacttcat cctgggcttt gttgccgcag cctggctcaa gttaccggtg
     6301 ctggctattg ctgccgctgc gctggcgatg gcgctgatcg acctgctgcg taaatctcct
     6361 gaaccgactc aacctgcggc acagaaagag gaatttgaag atggcatcta atcaaaccac
     6421 cctgccgaac gtcagtgaaa acgaagaaac attgctgact ggcgtcaatg aaaacgtgta
     6481 tgaagatcag agcattggcg cggagctgac gaaaaaagat atcaaccgcg tcgcctggcg
     6541 ttccatgctg ttacaagcgt cttttaacta cgaacgtatg caggcttctg gctggttgta
     6601 cggtctgctg cctgctctga aaaagatcca cactaataaa cgcgacctgg cgcgcgccat
     6661 gaaggggcat atgggtttct tcaataccca tccgtttctg gtgacatttg ttatcggcat
     6721 tatccttgcg atggagcgtt ctaagcagga cgttaacagt attcagagca ccaaaattgc
     6781 cgtcggtgcg ccgctcggcg ggattggcga tgcaatgttc tggctaacgc tactgccgat
     6841 ttgtggcggg ataggcgcaa gccttgcgct acaaggctct attcttggcg cagtcgtatt
     6901 tattgtgctg ttcaacgtgg tacacctggg gctgcgtttt ggtctggcgc attatgctta
     6961 ccgcatgggc gtggcggcga ttccactcat taaagcgaat accaaaaaag tcggccatgc
     7021 tgcgtctatc gttgggatga cggtaatcgg cgcgctggtg gcaacctatg ttcgtttaag
     7081 caccacgctg gaaatcacag cgggcgacgc agtggttaag ttacagactg atgttattga
     7141 caaactgatg cctgccttct taccgctggt ctacaccttg accatgttct ggctggtacg
     7201 ccgcggctgg agtccgctgc gcctgattgc agttaccgtg gttctcggca tcgtcggtaa
     7261 attctgccat ttcctttaaa tacaatgagg tattagatgt taagtattat tttgacaggg
     7321 catggcgggt ttgccagtgg catggaaaaa gcaatgaagc aaatcctcgg cgagcaatcg
     7381 cagtttattg ccatcgattt tcccgaaacc tcaagcaccg cgctgcttac atcgcagctt
     7441 gaagaggcca tcgcccaact ggattgtgaa gacggcattg tttttctgac tgatttgctg
     7501 ggcggcacgc cgtttcgcgt agcttcaacg ctggcgatgc aaaaaccggg ctgtgaagtg
     7561 atcaccggca ccaatttaca actgctactg gagatggtgc tggagcgcga agggttaagc
     7621 ggtgaagagt tccgtgtgca ggcgctggag tgtggacatc gtgggctgac cagtctggta
     7681 gacgagttag gccgctgcca tgaagaatgt ccggtcgagg aaggaatatg acacccgttc
     7741 tgcgcgccag aaggctgctg actgaagagg gatggctcga tgaccatcag ttgcgtattg
     7801 ctgacggtgt catcgcagca atcgaaccga ttccagtggg cgtgactgaa cgcgatgcgg
     7861 aactgctctg ccctgcttac atcgacaccc atgtacacgg gggtgcgggc gttgatgtaa
     7921 tggatgacgc gccggatgta ctcgacaagc tggcaatgca caaggcacgc gaaggtgtcg
     7981 gcagttggtt accgaccacc gtaaccgcgc cgcttaatac cattcatgcg gcgctgaaac
     8041 gtattgctca acgttgccaa cgcggcggac ctggtgcgca agtgctgggg agttatctcg
     8101 aaggaccgta cttcacgccg cagaataaag gcgcgcaacc gccggaattg tttcgcgagc
     8161 ttgaaattgc cgagctggat caattgattg ccgtttctca gcacacctta cgcgtggtag
     8221 cgctggcacc ggaaaaagag ggggcattgc aggccatccg ccatcttaaa cagcaaaatg
     8281 tacgagtgat gctggggcat agcgcggcga cctggcaaca aactcgcgcc gcgtttgatg
     8341 ctggtgccga cggcctggtg cattgctata acgggatgac aggtttacat caccgcgaac
     8401 cgggaatggt tggcgcggga ttaacggaca agcgcgcctg gctggaactg atagccgatg
     8461 gtcatcatgt gcatccggcg gcaatgtcgc tgtgttgttg ctgtgcgaaa gagagaatcg
     8521 tactgatcac cgacgcgatg caggcagctg ggatgccgga tggtcgctat acgttatgtg
     8581 gtgaagaagt gcagatgcac ggtggcgttg tccgtaccgc gtctggtggg ctggcgggca
     8641 atacgctgtc tgttgatgcg gcagtgcgca atatggtcga gttgacgggc gtaacgcctg
     8701 cggaagccat ccatatggcg tcgctgcatc cggcgcgaat gctgggtgtt gatggtgttc
     8761 tgggatcgct taaaccgggc aaacgcgcca gcgtcgttgc gctggatagc gggctacatg
     8821 tgcaacaaat ctggattcag ggtcaattag cttcgttttg atggtttgct ccattattgg
     8881 gccttcactt cccccgtaag gcctttcttt ttctttcttt ttgatctgtg cagcggtgtc
     8941 ggatgcgacg ctaacgcgtc ttatgcgacc tacggtacgc cgcatccggc aatcggtgaa
     9001 catagcgacg tcattacgca attctttaat cttcccttgt cttttctttc aacgatcaca
     9061 gatttcgttt tatttccttt ttcttcattg aactttctgt ttcttttcta tagattttaa
     9121 tcaacgaaag acatcaccaa gtgaaattaa acgaaaggca agtgaaagcg acaacgcccg
     9181 acgtcaagtt catcagacta aggattgagt tatgccagaa aattacaccc ctgctgccgc
     9241 cgcaaccggt acatggactg aagaagagat ccgccatcag cctcgcgcat ggatccgttc
     9301 actcaccaac atcgacgcgc tacgttccgc gctcaataac ttccttgaac cgttactgcg
     9361 caaagagaat ctgcggatca tcctgaccgg agccggaacc tcggcattta tcggtgacat
     9421 catcgcgccg tggctcgcca gccataccgg taaaaacttc agcgccgtac cgaccaccga
     9481 tctggtcact aatccgatgg actacctgaa cccagcccat ccgctgctgt tgatctcctt
     9541 cggtcgatcc ggcaacagcc cggaaagcgt cgctgccgtg gaactggcaa atcaatttgt
     9601 accagaatgc tatcacctgc cgatcacctg caacgaagcg ggcgctcttt accaaaacgc
     9661 gatcaacagc gataacgcgt ttgccctgct gatgcccgca gaaacgcacg atcgcggctt
     9721 tgcgatgacc agcagcatta ccaccatgat ggccagctgc ctcgcggttt tcgcacctga
     9781 gacgatcaac agccaaacct tccgcgacgt ggcggatcgt tgccaggcga tcctgacctc
     9841 actgggcgat ttcagcgaag gtgtgtttgg ttacgcaccg tggaaacgga tcgtttatct
     9901 cggcagcggt ggcttacagg gcgcagcacg cgagtcggcg ctgaaagtgc tggaactgac
     9961 ggcgggtaaa ctggcggcct tttatgattc tccaaccgga ttccgtcatg gaccaaaatc
    10021 gctggtcgat aacgaaacac tggtggtggt atttgtctcc agccaccctt acacccgtca
    10081 gtatgatctt gatctgctgg ctgaactccg ccgtgacaac caggcaatgc gcgtaatcgc
    10141 catcgccgcg gaaagcaccg acatcgtcgc tgccggtcca catattatcc tgccgccgtc
    10201 acgtcacttt atcgacgttg agcaggcatt ttgcttcctg atgtacgccc agacgtttgc
    10261 actgatgcag tcgctgcaca tgggcaatac gccggatacc ccatcagcca gtggcaccgt
    10321 taaccgcgtg gtgcaaggcg taatcattca tccgtggcag gcataagagg atcgcattat
    10381 gagcattatc tccactaaat atctgttaca ggacgcccag gccaatggct acgcggtgcc
    10441 tgcttttaac attcataacg ccgagacgat ccaggcgatc ctcgaagtgt gcagtgaaat
    10501 gcgatcgccg gtgatcctcg ccggaacgcc ggggaccttt aaacatatcg cgctggaaga
    10561 gatctacgcc ctgtgcagcg cctattccac aacctacaac atgccactgg cgctgcatct
    10621 cgaccaccac gaatcgctgg atgatattcg ccgtaaagtc cacgcaggtg tgcgcagtgc
    10681 aatgatcgac ggcagccact tcccgtttgc cgagaacgtg aagctggtga aatcggttgt
    10741 tgacttctgc cactcacagg attgcagcgt ggaagcagaa ctgggccgcc tgggcggtgt
    10801 tgaagatgac atgagcgttg acgccgaaag tgcattcctg accgatccac aagaagctaa
    10861 acgctttgtc gaactgactg gcgtcgacag cctggcggta gcgattggta cggcgcacgg
    10921 cttatacagc aaaacgccga agattgattt ccagcggctg gcggaaattc gtgaagtggt
    10981 ggatgttcct ctggtgctac atggtgccag cgatgttccg gatgaatttg tccgtcgcac
    11041 tattgaactt ggcgtcacaa aagtgaacgt tgccacagaa ttaaaaatag ccttcgctgg
    11101 cgcggttaaa gcctggtttg cggaaaatcc gcagggtaat gatcctcgtt attatatgcg
    11161 cgtcggaatg gatgcgatga aagaagttgt cagaaataaa attaatgtct gtggttcagc
    11221 gaatcgaatt tcagcataat cattagtttt ttatgattta tcccaatgta cttcccgatt
    11281 aatatcgggg agtgccttaa tggaaaagga gatgactaaa ccttaa
//
LOCUS       DAZFWP020000153        11173 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFWP020000153 DAZFWP020000000
VERSION     DAZFWP020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.153"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..97
                     /locus_tag="ACHK0Z_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             <1..97
                     /locus_tag="ACHK0Z_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4503928.1"
                     /translation="FFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            76..180
                     /gene="speFL"
                     /locus_tag="ACHK0Z_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             76..180
                     /gene="speFL"
                     /locus_tag="ACHK0Z_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU4503929.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            491..2689
                     /gene="speF"
                     /locus_tag="ACHK0Z_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             491..2689
                     /gene="speF"
                     /locus_tag="ACHK0Z_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGU4503930.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2686..4005
                     /gene="potE"
                     /locus_tag="ACHK0Z_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             2686..4005
                     /gene="potE"
                     /locus_tag="ACHK0Z_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU4503931.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4061..5701)
                     /gene="pgm"
                     /locus_tag="ACHK0Z_RS19965"
                     /old_locus_tag="pgaptmp_003993"
     CDS             complement(4061..5701)
                     /gene="pgm"
                     /locus_tag="ACHK0Z_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU4503932.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5727..6272)
                     /gene="seqA"
                     /locus_tag="ACHK0Z_RS19970"
                     /old_locus_tag="pgaptmp_003994"
     CDS             complement(5727..6272)
                     /gene="seqA"
                     /locus_tag="ACHK0Z_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU4503933.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6457..7221
                     /gene="ybfF"
                     /locus_tag="ACHK0Z_RS19975"
                     /old_locus_tag="pgaptmp_003995"
     CDS             6457..7221
                     /gene="ybfF"
                     /locus_tag="ACHK0Z_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4503934.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7361..7654
                     /gene="ybfE"
                     /locus_tag="ACHK0Z_RS19980"
                     /old_locus_tag="pgaptmp_003996"
     CDS             7361..7654
                     /gene="ybfE"
                     /locus_tag="ACHK0Z_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU4503935.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7794..8324
                     /gene="fldA"
                     /locus_tag="ACHK0Z_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             7794..8324
                     /gene="fldA"
                     /locus_tag="ACHK0Z_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU4503936.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8534..8620
                     /gene="uof"
                     /locus_tag="ACHK0Z_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             8534..8620
                     /gene="uof"
                     /locus_tag="ACHK0Z_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU4503937.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8613..9059
                     /gene="fur"
                     /locus_tag="ACHK0Z_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             8613..9059
                     /gene="fur"
                     /locus_tag="ACHK0Z_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU4503938.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9143..9469)
                     /gene="chiQ"
                     /locus_tag="ACHK0Z_RS20000"
                     /old_locus_tag="pgaptmp_004000"
     CDS             complement(9143..9469)
                     /gene="chiQ"
                     /locus_tag="ACHK0Z_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU4503939.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9519..10925)
                     /gene="chiP"
                     /locus_tag="ACHK0Z_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             complement(9519..10925)
                     /gene="chiP"
                     /locus_tag="ACHK0Z_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGU4503940.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2706 a         2759 c         2794 g         2914 t
ORIGIN      
        1 cttcttcaca gattatagcc atttcatgga tagaataact ctaccttcaa ctgacacagc
       61 aagaggtaaa ggtaaatgga aaataataac cgcttaatgc ctcatataag gcggacaacc
      121 catatcatga agtttgccca tcgtaatagt tttgactttc atttctttaa tgcccgctag
      181 tcttctgact aaagggcacc ccaacgtaca ggtctccctg actttaagca ttacaggtta
      241 atacctgtat tcctcggtgc tcatatactg ctaacccttt taaactctaa ataattcgag
      301 tcgcagcact tgcaacttga ggtatgacga gtatagccag ttaccgggct ggtctgggtt
      361 attgcatctg caaaaagcaa actactgatt tatttatcag cggtggagct ttgctttttt
      421 tccggcgtga tcgatttctc ctttgagaaa ttgaggacct gctattacct gaaataaaga
      481 gatgaacaaa atgtcagaat taaaaattgc cgttagtcgt tcttgcccgg attgtttttc
      541 cactcatcgt gcatgcgtga atatagacga aagtaattat attgacgttg ccgccattat
      601 tttatcagtc agtgatgttg aacgtggaaa actcgatgaa atagacgcta ctggctatga
      661 cattcctgtt tttattgcaa cggaaaatga agaacgtgta cccgcagaat atctgccacg
      721 tatttctggt gtcttcgaac attgcgaatc acgtaaagag ttttatggac gtcagttaga
      781 aaccgctgcc agccattatg aaacgcaact gcgcccgccc ttcttccgcg cactggtcga
      841 ttatgttaat cagggcaata gtgcttttga ctgccctggt catcaggggg gtgagttttt
      901 ccgtcgccat ccggcaggca atcagttcgt ggaatacttt ggcgaaatgc tcttccgctc
      961 tgacttgtgt aatgctgacg tagcaatggg cgatctgctg attcatgaag gtgcgccatg
     1021 tattgcacaa cagcatgcgg ctaaagtctt taatgccgat aaaacctatt tcgttttaaa
     1081 cggtacttca tcttctaata aagtggtctt aaacgcattg ctgacgccgg gcgatctggt
     1141 gttgtttgac cgcaataacc ataaatctaa ccaccacggt gcgttgctac aggctggcgc
     1201 gacaccggtt tatctggaaa ccgcacgtaa cccgtatggc tttatcggtg gcattgatgc
     1261 gcactgtttt gaagaaagtt acctgcgtga gctgatcgcg gaagtcgcac cgcagcgggc
     1321 aaaagaggct cgtcctttcc gcctcgctgt gattcagtta ggcacctacg acggtacgat
     1381 ttataacgcc cgccaggtgg tggataaaat tggtcatctg tgtgactaca ttctgtttga
     1441 ctccgcatgg gttggctatg agcaatttat cccgatgatg gctgactgtt cgccgctgtt
     1501 gctggatctt aatgagaacg atccgggtat tctggttacg caatccgtac ataaacagca
     1561 ggctggtttt tctcaaactt cacaaatcca taaaaaagac agccacatca aagggcaaca
     1621 gcgttatgta ccgcacaaac gcatgaacaa cgcctttatg atgcatgcct ccaccagccc
     1681 gttctatccg ctgtttgccg cactggatat caacgccaaa atgcatgaag gtgtcagcgg
     1741 tcgtaatatg tggatggatt gtgtggtaaa tggcattaat gcccgcaagc tgatcctcga
     1801 taactgtcag catattcgtc cgttcgtacc tgaactggtg gatggtaaac cctggcagtc
     1861 gtatgaaaca gcgcaaattg cggttgatct gcgcttcttc cagtttgtac caggtgagca
     1921 ctggcactct tttgaaggct atgcagagaa tcaatacttt gtcgatccat gcaaactgtt
     1981 gctgacaacc ccaggtattg atgcacgtaa cggcgaatat gaagcgttcg gtgtacccgc
     2041 gacgattctt gctaacttcc tgcgcgaaaa tggcgtagtg ccggaaaaat gcgatcttaa
     2101 ctccatcctc ttcctgctga ctccggcaga agatatggcc aagcttcagc aacttgttgc
     2161 cctgctggta cgcttcgaaa aactgcttga gtccgacgcg ccattagcag aagtgctgcc
     2221 ttccatctac aaacagcatg aagagcgcta cgccggttat accctgcgtc agttgtgtca
     2281 agaaatgcat gatttgtatg cccgtcacaa cgtgaaacaa ctgcaaaaag agatgttccg
     2341 taaggaacac ttcccacgcg taagcatgaa tccgcaagaa gccaactacg cctatttacg
     2401 cggtgaagtg gagctggttc gtctgccgga tgcagaaggc cgtatcgctg ccgaaggtgc
     2461 gcttccttat cctccgggtg tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt
     2521 tttgcgttac ttcagcgctc tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga
     2581 gctgcagggt gtctatatcg aagaacatga tggtcgtaag caagtttggt gctatgtcat
     2641 caagcctcgt gatgcgcaaa gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa
     2701 tcgaacaaaa tgggcgtcgt tcagttaacc atactgacga tggtcaacat gatgggctcc
     2761 ggtatcatca tgctgccgac aaagcttgcc gaagttggga caatttcaat tatctcctgg
     2821 ctggtgacag ccgtaggctc aatggcactg gcatgggcat tcgcaaaatg cggtatgttc
     2881 agccgtaaat caggcggtat gggcggttat gcggagtatg ctttcggtaa atccggtaac
     2941 tttatggcga actataccta cggcgtctcg ctgctgattg ctaacgtcgc gattgctatt
     3001 tcggcggttg gttacggcac cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt
     3061 cttgcgacca tcggggtgct gtggatttgt accgtggcta actttggtgg tgcgcgcatt
     3121 accgggcaaa tcagtagcat taccgtgtgg ggggtcatta ttccggtggt aggcctgtgc
     3181 attatcggct ggttctggtt tagcccgacg ctttacgttg attcctggaa tccgcatcat
     3241 gcaccgttct tcagtgcagt aggttcttcc atcgctatga cgctgtgggc ttttctcggt
     3301 ctggagtctg cgtgtgcgaa tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc
     3361 gcggtactcg gcggtacgtt aggtgcggcg gtgatttata tcgtctccac caacgtgatt
     3421 gcagggattg tgcctaatat ggagctggca aattcaacgg caccatttgg cctggccttc
     3481 gcacagatgt tcacaccaga agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc
     3541 tgcggttcgc tgcttggctg gcagttcacc attgcccagg tgtttaaatc ttcatctgat
     3601 gaaggctact tccctaaaat tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga
     3661 atgttgacca ttgtgattat ccagagcggg ttggcgctga tgacgattag cccgtcgcta
     3721 aacagtcagt tcaacgtgct ggttaacctg gccgtggtga ccaatatcat tccgtatatt
     3781 ctgtcaatgg cggcattagt cattattcag aaggtcgcta atgtgccccc ctcaaaagcg
     3841 aaagttgcaa actttgttgc ttttgttggc gcgatgtata gcttttatgc gctgtactca
     3901 tccggggaag aagccatgct gtacggttcc atcgtgacct tccttggttg gacactgtat
     3961 ggtctggtat caccacgctt tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa
     4021 aagggcgatc ttgcgaccgc ccttttttta ttaaatgtgt ttacgcgttt ttcagaactt
     4081 cgctaacaat ctcaaccgct tctttctcaa tctgcttgcg atgttcttca ccgaggaagc
     4141 tttcgcagta gatcttatat gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt
     4201 tgtcagtcat cactttcaga ccgccgatag aagcaccatt acccggtgcc gccgtcagac
     4261 gtgcggtgat cgggtcacct gccagggtgc tggcgctcac catttccgga gacagcttag
     4321 acaacgccgc tttttgtgcg gaagtcgcag cggcctgcaa acggttgtag ctcggcgcac
     4381 caaagcgttt tgccagttcg ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga
     4441 tttccgccgc cagcagacac ataatgatgc cgtctttatc ggtggaccac ggcgtgccgt
     4501 cgaaacgcag gaaggaagcc ccggcgctct cttcgccgcc aaagccgaaa ctgccgtcga
     4561 acagaccatc aacaaaccat ttgaatccta ccgggacttc taccagttta cggcccaagg
     4621 cgttgaccac acggtcgatc atcgcagaag aaaccagcgt tttaccgaca gcaacatctt
     4681 tgccccactg cggacgatgc tggaacaggt agttgatcgc caccgccagg tagtggttcg
     4741 gattcatcaa acctgccgga gtgacgatac cgtgacggtc ataatccggg tcgttagcaa
     4801 acgccagatc gaacttatca cgcagtgcca gcaggcccgc catcgcacac tcggaggagc
     4861 agtccatacg gatcgcgccg tctttatcaa ggtgcataaa gcggaaggtt tgatcgacct
     4921 gatcgttaac gatggtcagg ttgaggttgt aatactcgcc aatacgcttc cagtattcga
     4981 taccggaacc gcccagcgga tcaacgccaa gcgtcaggcc cgctttctga atcgccgcca
     5041 tatcaacgat atcggccaga ccttccacaa acggctgcac cagatcctgc tctttcacat
     5101 gaccggatgc catcgcttcg tcgagagaga tacgcttcac gcctttcagg ccatcggcca
     5161 tcagtgcgtt ggccctgtct tccaccactt tggtgacgtt ggtatcagcc gggccaccat
     5221 ttggcgggtt gtacttgata ccaccatctt ccggcgggtt atgggacggt gtaatcacga
     5281 taccgtctgc cagcgggcca ccttttttat tgtgaaccag gatggcattg gaaacggcag
     5341 gcgtcggagt gaatccattg ttttcctgca caatgacatc aacgccgttc gctgccagca
     5401 cttccagtac ggaaatgaac gcaggctcgg acagggcgtg agtatcttta cccacatagc
     5461 aagggccagt gatgccattt ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa
     5521 tgtgcggctc gttaaagctg tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt
     5581 tcaccgcgtg ctccgcattc cctgcttctg gtttcagtac ataatattgc gccgtcagtt
     5641 gggcgacgtt aatcaaatca ctctgttgtg caggttggcc tgcacgattg tggattgcca
     5701 ttgctttgtc ctttgtctgc aacgttttag atagttccgc gaaccttctc aatcaattcc
     5761 gccgggaatt gcatcgactg catgatgtgt tcgatcatgc tgcatttacg gccggtgttg
     5821 gtgttggtga tcacccaata cggcgtgcct ggcacatgtt tcggcttggt ctgattacca
     5881 tttttcagca gcgtttgttc atctgccgca aagtaaacgc gtgtacgacc gtgcaacgat
     5941 tccgttgctt cggcaaacgc ctgggcgtca agagaatata gtgtagacaa cagcagcata
     6001 aagcgattga ccgctcgctt ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt
     6061 gcgcgaacct tgtctttaat cgttttgacc ggcttcgctt cgacgatagc aggtgacgca
     6121 acgcgaacct ctttcgtcac cggagcagca ggctgtgatg aggcggaaaa tttcaacata
     6181 cgccgtaaaa tgtcggatgc gctctcgccg atatgcttag tgtggctggc aatatagctg
     6241 tagagttcat catcaacttc aatcgttttc atcttaatcc agtgcagtgt cttagctgaa
     6301 tacaagtcgc caggagtata tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg
     6361 cagaaaaact cggaataaac cttatttgtt gcagccctgt ggcacaatgg tcaacataat
     6421 accctaaccc gacaacgcga aaaaaagaac tttgccatga aattgaatat ccgcgcgcaa
     6481 actgcacaaa accagcacaa taattctccc atcgttcttg tccatggtct gtttggtagc
     6541 ctcgacaacc ttggcgtact ggctcgcgat ctggtaaacg atcacaatat catccaggtt
     6601 gatatgcgta accacggtct ttcaccgaga gatccggtaa tgaattaccc ggcgatggcg
     6661 caggatcttg ttgatactct ggatgcacag cagatcgaca aagcaacatt tatcggtcac
     6721 tccatgggcg gtaaagcggt aatggcactt actgcactgg cccccgatcg catcgataaa
     6781 ctggtggcga tcgatatcgc accggtcgac tatcacgtac gccgtcatga tgagattttc
     6841 gcggctatca acgcggtcag tgaatcggac gcacaaactc gccagcaagc agcagcaata
     6901 atgcgccagc atcttaatga agaaggggtg attcagtttc tgttgaaatc ttttgttgac
     6961 ggggagtggc gctttaacgt gccggtattg tgggatcagt atccgcatat tgtgggttgg
     7021 gagaaaatcc cggcatggga tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat
     7081 gttagcgagc agtaccgtga tgatttactg gcacaatttc cacaggcacg ggcgcatgtg
     7141 attgcaggcg cgggtcactg ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc
     7201 cgtcgctatc tcaatgatta acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg
     7261 cgcgtgggcg ttggcgtgac tcatcgcgct gatgtattat ggcgcgctat ccatccgggc
     7321 agaggcctgg ctgattgttt cccccgaagt caccaagatc atggccaaag aacaaacgga
     7381 ccgtacgaca ttagatctgt tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc
     7441 gctttcgcgc gatgaacagc tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt
     7501 acgtggcctt aagcgtgtcg aactgaagct gaacgcggaa gctgtcgagg cgctgaacga
     7561 gctggcggag tcgcgcaata tgagccgtag cgaactgatc gaagagatgt taatgcagca
     7621 actggcggcg ctgcgtagtc agggcatcgt ttaaatttcc actttcatgt agcacagtgt
     7681 gcagtcctgc tcgtttgctg attccgcaag actgcctgtt ctgctatgat tgcctttatc
     7741 cgtgggcaat tttccacccc catttcaata agtttcaaga ggttatttca ctcatggcta
     7801 tcactggcat ctttttcggc agcgacaccg gtaataccga aaatatcgca aaaatgattc
     7861 aaaaacagct tggtaaagac gttgccgatg tccatgacat tgcaaaaagc agcaaagaag
     7921 atctggaagc ttatgacatt ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc
     7981 agtgtgactg ggatgacttc ttcccgactc tcgaagagat tgatttcaac ggcaaactgg
     8041 ttgcgctgtt tggttgtggt gaccaggaag attacgccga atatttctgc gacgcattgg
     8101 gcaccatccg cgacatcatt gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg
     8161 cgggctatca tttcgaagca tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg
     8221 ctatcgacga agaccgtcag ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac
     8281 agatttctga agagttgcat ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga
     8341 ctcatgtcta cgccgtatta atagataatg ccaatcaaaa taattgctac aaatttgtaa
     8401 cttttgctgt tgtacctgta caatgtcccg gtgttcaagt ggccttgccg ttgtaaatgt
     8461 aagctgtgcc acgtttttat taacaatatt tgccagggac ttgtggtttt catttaggcg
     8521 tggcaattct ataatgatac gcattatctc aagagcaaat tctgtcactt cttctaatga
     8581 agtgaaccgc ttagtaacag gacagattcc gcatgactga taacaatacc gccctaaaga
     8641 aagctggcct gaaagtaacg cttccacgtt taaaaatcct ggaagttctt caggagccgg
     8701 acaaccatca cgtcagtgcg gaagatttat acaaacgtct gatcgatatg ggtgaagaaa
     8761 ttggtctggc tacggtatat cgcgtactga accagtttga cgacgctggt atcgtcaccc
     8821 gccacaattt tgaaggcggt aaatccgtat ttgaactgac acagcaacat caccacgatc
     8881 acctgatttg cctcgactgc ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc
     8941 gtcagcgtga aattgccgca aaacatggca ttcgcctgac taaccacagt ctctatcttt
     9001 acggtcactg tgccgaaggc gattgccgcg aagatgagca cgcgcacgaa ggcaaataag
     9061 ccagcctgaa cgagaaaagc caacctgcgg gttggctttt ttatacaagg gaaataagaa
     9121 aagcgagagt tacagctctc acttatttgt tattactgcg tatttcctgc cagaccttat
     9181 cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga cactgctgat
     9241 aatccagtac gcgtacactt tcctggtcgg caaactgctg atgttgtttc tctttcttca
     9301 gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt gcggtgttga
     9361 tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt gattgcgcac
     9421 aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata gcaaattcct
     9481 caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt gaatggtgcg
     9541 attaccataa atttcacgtc acgctcatcc tggaagatgt taccgtaacc gccgccccag
     9601 cttgggatgt cagagtggtt gtcgtattcg gtgaagtgca gtttgaacat cgtgcctttg
     9661 gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt aggcagactc ttcaatagtc
     9721 cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt agcatcccat
     9781 gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt caggtcatac
     9841 atcgcaccga agaagaccgc tttttcgccg ttggcgttga agtcggaacg gttatcccac
     9901 cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg caggaagtat
     9961 ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac tacgtcagcc
    10021 gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag gtcgttgacg
    10081 ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt taacgggcta
    10141 ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata cccttccgcc
    10201 tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc accgatggag
    10261 tgcaggtaat caactttggt ggttttatcg ttctgataaa actcatccat ttccagatgc
    10321 cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaaggcacc agcatcgccg
    10381 taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa gctccagtgc
    10441 ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca aaccggaccg
    10501 tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc ggaccagtct
    10561 tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga gctgtcgccg
    10621 ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat atcagcagca
    10681 taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt ggttttgtat
    10741 ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata tataccgccg
    10801 gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc cataaagccc
    10861 gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc actaaacgta
    10921 cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac agcgaaaaaa
    10981 taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca gactattttt
    11041 cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga gcaaccgcac
    11101 atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt gcagaagaat
    11161 aaaagcgtga att
//
LOCUS       DAZFWP020000154        11150 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFWP020000154 DAZFWP020000000
VERSION     DAZFWP020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.154"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1216
                     /locus_tag="ACHK0Z_RS20010"
                     /old_locus_tag="pgaptmp_004002"
     CDS             <1..1216
                     /locus_tag="ACHK0Z_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503941.1"
                     /translation="TAMSQHTDTSNLEIISTAIETLRTQIALIQKRNPGDDLSKRLHE
                     SVIATTDNLVAEINKLLDDGAVDYNKLVDQFEEYQQAVNDGLIRFSQVTGVSATVESL
                     GDAVNQFAANMRNEIGNLEACLEQANTLRKSAEADLNRYKKDYPPSLTKRLDVAEKDN
                     RTLKRERRELKERLTKLNQQCIDYQGEGVTLRKKLATAQSIIETLKRECSQLGHDLNR
                     ACGMGQRPETFPLMYDGRDAIAYIHEYPHGLVAETGQRGEALLTANYHQQIRTNRLLT
                     MDVIPSVWGTPLYYRLPGFEKDWNTDIDECLADKIMAYLETDFPRLYRRIMDSKDAPI
                     DELKMRPETLEAIKQTEFDTVFSVACIPSCFHESIPFMQGDRRQEIIDACRVWANEWD
                     KKNGGVEDLYGK"
     gene            2452..2528
                     /locus_tag="ACHK0Z_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     tRNA            2452..2528
                     /locus_tag="ACHK0Z_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2486..2488,aa:Arg,seq:tct)
     gene            2534..2620
                     /locus_tag="ACHK0Z_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     tRNA            2534..2620
                     /locus_tag="ACHK0Z_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2568..2570,aa:Tyr,seq:gta)
     gene            2625..2700
                     /locus_tag="ACHK0Z_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     tRNA            2625..2700
                     /locus_tag="ACHK0Z_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2658..2660,aa:Thr,seq:tgt)
     gene            3639..3725
                     /locus_tag="ACHK0Z_RS20030"
                     /old_locus_tag="pgaptmp_004006"
     tRNA            3639..3725
                     /locus_tag="ACHK0Z_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3671..3673,aa:Ser,seq:tga)
     gene            4206..5033
                     /locus_tag="ACHK0Z_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             4206..5033
                     /locus_tag="ACHK0Z_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4503942.1"
                     /translation="MKKTLISAAIIFGSLCLTGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDDPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            5188..5263
                     /locus_tag="ACHK0Z_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     tRNA            5188..5263
                     /locus_tag="ACHK0Z_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5221..5223,aa:Ile,seq:cat)
     gene            5718..6293
                     /locus_tag="ACHK0Z_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             5718..6293
                     /locus_tag="ACHK0Z_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503943.1"
                     /translation="MLMANDNAYAEEDLILSDFIGKRERWTQKREELYASLVRKGVNI
                     ETAQSGDMTVVSVGLHGVSVSAINHEPYVALSESMVRLVKFLKYTEANNVIIGKKNIP
                     FSSAFYWMMKGLDARRTSWPKGSYISMFRGSIGSKEKLFEFLPEEAFDIVEGCDVMVM
                     PRLVMMNGDLQAQTDWFATGVDIIATDWEAF"
     gene            6443..6685
                     /locus_tag="ACHK0Z_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             6443..6685
                     /locus_tag="ACHK0Z_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503944.1"
                     /translation="MRVRFRWKAEQVTPFREPSMPLQVEQDYLCLPEIDGGMLVESDG
                     SKADFEANLKLAQAVINSYRGKWIAKVKHVVRLSRW"
     gene            6794..7186
                     /locus_tag="ACHK0Z_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     CDS             6794..7186
                     /locus_tag="ACHK0Z_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late promoter activating protein"
                     /protein_id="HGU4503945.1"
                     /translation="MEQISSAEIADIMIRADCYLTVTEITTLAKEKYPHLHVSRVSVT
                     NIIRHFVRSSRAICELDDRVYPRKYWLHGLNGYQFKVRGRTPEYGSLLVKNCSRKSVE
                     QARKEQRELVDMANKLWNAAVKKRGVAL"
     gene            7183..8547
                     /locus_tag="ACHK0Z_RS20060"
                     /old_locus_tag="pgaptmp_004012"
     CDS             7183..8547
                     /locus_tag="ACHK0Z_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA pacase A subunit"
                     /protein_id="HGU4503946.1"
                     /translation="MSLVNWEEHRARFIALREEKGITVKEYCEEHGLSFNTARKHLNM
                     KKNEVRSQVKTTKKSTKSPEKKRAGRATKKLEEGAEKTKFTEIDNSQGAGSFRSMPRK
                     KTSDHESNTNNNNEIKESVRRSLREKMAKMIPEEDKSAPKIEGSSAMIPGGAVQRATL
                     PTTNVARDMMKNGAEEHLRLAIQMAQERALQYQSIVDQEAERLQAEIDALGDKEPEGM
                     HPGQRLLGLISDAAYYMNDFISRLAAIYQSEQKLRQGDEKLRQSARQQAFKEAEAREK
                     LELARLQAEQRGKEIEYRIGADARAARVIAAAIRMRDREELDDIGVAEYIERQGVSVP
                     AILAARAAKAITLLEPPVSDVNDVDDEQLDKEAREFATLQANHPQWLAERRADVATIV
                     EELGCGDYDRNGERKAGEFEANDEELDIDPSATAEIYGDYDASDAGYDAGDDDIAIEP
                     PEDD"
     gene            8547..10055
                     /locus_tag="ACHK0Z_RS20065"
                     /old_locus_tag="pgaptmp_004013"
     CDS             8547..10055
                     /locus_tag="ACHK0Z_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4503947.1"
                     /translation="MAGGRKIKCVTSDPRWRDMVIKYRYNFTQAVVDIFGMFPSHQQQ
                     QIIQSVQETGSRTTVTSGHGTGKSSLTAMLLLIFMILFPDARVIIVANKIGQVKTGVF
                     KYVKQYWANAVKRHGWLQTYFVLSDTMFYERSRKGIWEVLCKGYRLGNEEALAGEHAA
                     HLLLILDEASGISDKAIGVMTGALTEEDNRMLMLSQPTRPSGYFYDSHHSQAKTPDNP
                     KGIWTAIVLNSEESPFVTPQFIKQKLLEYGGRDSIEYMVKVLGQFPREINGYLLGRDE
                     CDRAARRKVLLEKNWGWVATADVGNGRDKSVLNICKVSGHRDKRRVVNFKVMEMPGTM
                     DPLAFADFIYNECTPEKYPNITIAVDADGFGSDTCAQLVRRGANPVRIRWGKPMFANK
                     DRERFVNQRAYANIMARDAIKSGRMRIDSDPKTAEQASKIPFLLNEEGKMAMMRKEHM
                     RQKLNIKSPDRWDTYCFTMLVDYVPANEDIGAEMATFRDQVLADIEMPDLDI"
     gene            complement(10083..>11150)
                     /locus_tag="ACHK0Z_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             complement(10083..>11150)
                     /locus_tag="ACHK0Z_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503948.1"
                     /translation="YVRKLNRVGIMSKKSIEKEYKRFLQTAERWKELVVANSVFHDTS
                     YAGEEFRHVALTHDQNILEEAEKCLAEWKAFVDMCRDADGKASNIVESVYSPIPFIIE
                     DTNQSTHVVVQSATTTRTFTREQLLKKYDKIIKKSLKNRVFSQIVGALEEEQRFFEAE
                     PEGEIYRARKEAYTDVVLTTNIEGSNALSRFRVGAHGALVFARLPKTTIPVVNNVGER
                     RSITIYSGVESVPCSLLGDFNLYRVRDLEKHQPSYVAKSYILRNIDIRNESLKQKSAK
                     MLEDADPAIRHIIERKIRTSREAMARLDKMDLELLDVMMASGDDLTGIKLNEARKKYG
                     KAIEERYGYTFPQTQYAAKLW"
BASE COUNT         3023 a         2497 c         2957 g         2673 t
ORIGIN      
        1 aacagcaatg agtcaacaca ctgatacttc taatctggaa attatctcga cagcaattga
       61 aacgctgcgc acacaaatcg ctcttataca gaaacggaat ccgggtgatg acctgtcaaa
      121 gcgactgcac gagagtgtca ttgccactac agataacctg gtggcggaaa tcaacaagct
      181 gcttgatgat ggggcagttg attacaataa gctggtggat cagtttgagg aataccaaca
      241 agccgttaat gatggcttga tccgtttttc acaagtcaca ggcgtatcag cgacagttga
      301 aagcctgggc gatgcagtta atcaatttgc cgcgaatatg cgtaatgaga tcggcaatct
      361 ggaagcgtgt cttgaacagg caaatacact gcgtaaatct gccgaagccg atttgaaccg
      421 ttacaaaaaa gactatccgc ccagcctgac aaagcggctg gatgttgcgg agaaggataa
      481 ccgcacgctg aaaagagagc gccgggaact aaaagagcgt ttaaccaagc tcaatcagca
      541 atgcattgat tatcaaggcg aaggcgtcac actgcgtaaa aagttggcta cagcgcagag
      601 cattatcgaa acattaaagc gagagtgctc acagcttggt cacgatctga accgtgcttg
      661 tggcatggga caaagaccag agacatttcc acttatgtat gacggaaggg atgccattgc
      721 ctacatccat gagtatccgc acggcctggt tgcggaaact gggcagcgtg gcgaagcact
      781 gctgacggct aattaccatc aacaaatcag aaccaatcgc ctgttaacga tggatgtgat
      841 cccatcggta tggggaacgc cgctttacta tcgcctgccg ggcttcgaaa aggactggaa
      901 taccgacata gacgaatgtc tcgcagataa aattatggcg tacctggaga cggattttcc
      961 gcgcttatat cgccggatta tggactctaa ggatgcgcca atcgatgaat taaaaatgcg
     1021 tccggagacg ctggaggcaa tcaagcagac ggagtttgat actgtattca gtgtggcctg
     1081 cattccaagc tgtttccatg aaagcattcc gttcatgcaa ggagatcgtc gtcaggaaat
     1141 cattgacgct tgccgggtat gggcgaacga atgggataag aagaatgggg gtgtagaaga
     1201 tctttacggg aaataatatg ttagaaatct aaaatcacaa ttcgaatgta ttgattgtga
     1261 aggtaactta acatagaatt ttccttgctg gtgggcggta cccacgtttc gcttgctggg
     1321 cttgatagtc acttattggt tattgttcac ccaccagcat ttaacaaatt aaagtgttag
     1381 aaaagtatag taacgcaaga gcacgctttg gcaccgtgca ggccaaagtt cagtaggttt
     1441 agtgcggaaa gtggtttcag agagagcact ttgcggagta aacgacaata catgttgagc
     1501 cacggaaggt ctttttaagg tgttcattga tgatggtccg gcagagagta acggtacatg
     1561 ctgcatctaa tcatcaatgc acatcttcaa cattaaggag gcatagggat ttttgtggcg
     1621 gttacctttt gccctgagtc tccttaatgt tgcagtaatg cacctacgcg gatgtggcct
     1681 tgcattgtgt cttgaaagaa tggggtaagg ccggggatat ccccaggggg aggcaccccc
     1741 gctgcaacga acagccacag acggcagtcg agtgatggaa acgtgcaaag actttaaacc
     1801 tcgttacggc tggcgtggca aacgcgacaa ccgagacaga gcggtgacgg gcggagagag
     1861 tgccgcatca agaaagaagc cgacttgtga tccctttcag cgggcatttg aaagtgaatc
     1921 cagctcaggc agtcggcttc tttcttgtgt gagatgtagc gtatttgccc atcttcgggt
     1981 gggctttttt agggttttcg tcatggttag cgactttgcg gcggtttaga aaccgaccat
     2041 taaagtaaat gcaaacgatg atctgatgat ggtagcggcc taagaagcca gacgccacgg
     2101 ggtatgagtc gtcccccgtc aaaaaatcga ccgcagagtg tccccgtctg tgtattaggg
     2161 aacggggagg cataacaggc aagggcgctg gtgtgattaa ccagatgaat gagaagggtc
     2221 aggtctttct ggtcagcgcc cttacctgtt acgtccctcc cgcgtaacag ccattgctta
     2281 atggcatcct gggggaattt cttgtaacgg gtatatcccg tcatgctgaa ggcgctaatc
     2341 acgctggaag ccagggttat gcatcccctg ttactgaatt gcagccaggg cgcggtgcgc
     2401 cgaaaagcat acggaggtgg aagccctcgc cggagacgta cccggcacag tggtggtgta
     2461 gctcagtagg atagagcagc agccttctaa gctgtcggtc ggtggttcga atccacccac
     2521 catcgccaat ttaggggagt tagtccgtag gggtagcggg gtagactgta aatctactgt
     2581 cattgcgact cggatggttc gactccatca ctccccacca aattgccggt tttgctcagt
     2641 tggtagagcg cctgccttgt aagcaggatg tcagcggttc gagtccgtta accggcacca
     2701 acacaacagg taagggtatt ttgcgacgtc ggagatcgcc gagcttggca gagggttcga
     2761 atccctacga agtaccctta ccgttgtgat gaatgcgcag gctgatgcgc gaaagacatt
     2821 gcagctattg cggaaaagag ctgtccggcg gggcaattaa acgcccgtga gagtctgaaa
     2881 taaccgcaag ccggagatca gcaccggtca tcacaacaca acaggtaagg gcattctcct
     2941 tatggggctt ggcttaaatg catcgagtgc tcttaccgtt gtgatgaagt gcagctctta
     3001 gaagcaacca gaagataagc atctggcttc acaacataaa ccgcaggaac gaccaataaa
     3061 cggtagtccg tatggagaac accccgttga ggaagaggcc tggccggaac cgtaaccggc
     3121 actacaacgt tgagaacact ggcgtaacgg ggtcatatcc caatctatga ataaatgttg
     3181 cgttgccgcg tgacaaccag tgttctcaac attgtggtga atgcacaggc tgatgtgccg
     3241 caactacagt agtgcgcgct ttgcggggct tgctacaacc ctgtgtcgga gttcagcacc
     3301 gaccatcaca gtttgattct ctggcatgag cataacgctg aaataagtcc agtctggtgc
     3361 ggtctgatca cccgccgtta gctccacgaa acggagcgca caacaggtaa gagtatttgt
     3421 agagttcgac tctctaccgt gggctttttc ccgcgatgcg agccataaat gctctttccg
     3481 ttgtggtgaa tgcgcgttgc tggcgcgcaa gtccgcccta tggcaacgag gcggcgtagt
     3541 aaatatcgat gcctcagtca tcaagcgtac tgaccggcct tatgggctat gaaaaaaaga
     3601 ggcgcagtcg gcgatcagca ccgaccatca caatcactgg agagtaggga gcatggtgct
     3661 caagcggtct tgaaaaccgt cccattgcgc aagcgatgat ggttcgattc cattactctc
     3721 cgccagcgca acgttaagag tatttggcta attaagcgaa tgccggaagc agaaccggat
     3781 caccaaatgc gtacaggcgt catcgccgcc cagccagcca agtgctctta ccattgcggt
     3841 gaatgcggct aagcgcacgc ggaacagtta aaacaatttt cctcaatagt taagtcaaac
     3901 atcccggcgt taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcagcttt
     3961 tttattcgtt aaataatgga gtgagaggat gcagaacaat ccgaacaaat gtcgaacgct
     4021 atgggtgcgg ttatatattt atgccgtcct ctgtttgatt gtgtcactgg ttctgtatgt
     4081 ttggcttttg ccaaatatga tctcatctga cagcacaata cttgtattgt tgggagtcct
     4141 tctcgcgctc atttacccgg cttttgcagt agtctttttt cgtgaaaaaa ccaggaaatt
     4201 gattaatgaa aaaaacgttg attagtgcag cgattatttt tggttcttta tgtctgaccg
     4261 gatgcgaccg ggtagaacca ggcaatgtag ggatcaaagt aaataagctg ggggatgata
     4321 aaggtgtcgg cgaagtagtt ggcgttggtc gctactggac aggctggaac acagaagttt
     4381 atatcttccc gacctttaag caaatgaaga cctatgatga tccgttcagc ttccaaatga
     4441 gcgacgggac aacaattggt taccacattg gtgtagctta caaggttgat ccggcaaaag
     4501 tcacaactgt atttcagacc taccgcaaag gcgtggatga cattaccgac accgacctgc
     4561 gccagaagat tgccgacgca cttaaccgac tggccagcaa aatgaccacc gacaaattta
     4621 tcgacggtgg caagtctgaa ctactggatg cagctcttaa agacatccag gaagaaatga
     4681 cgcccatcgg tattcaggta atgagcctct catatgtggg caagccggag tacccgccaa
     4741 ccgttatcga cagcattaat gccaaagtca cggcgaacca gaaaactctg caacgcgagc
     4801 aggaagtcaa gcaacgcgaa gcagaagcca acatgctgcg cgcagaagct gccggacagg
     4861 ccgatgccat tcgcacaaaa gcccaggctg aagccgatgc cattcgttta cgcggtgagg
     4921 ctctgcgcca gaaccccggc gttatggagt tggaagcgat caacaaatgg aacggaacgc
     4981 taccgcagta tatgaccagt aataccgctg ttccgtttgt tccggtgaaa taaccaaata
     5041 gataacatga tggaattttg gacgtttgcc gacaagaacc cttggtggac atttttattt
     5101 ctgctggtgg ttgcatatgg ggtcgagcaa gcagcaaaag ccttacgcgg tcctggcaaa
     5161 aagcgtaagc ggaattaaca gtaatctggc cctttagctc agtggttaga gcaggcgact
     5221 cataatcgca cggtcaccgg ttcaaatccg gtaggggcca ccatatttgg ttgtaacacg
     5281 gcgtctggca catgcgtcgt tagcggtctg gtgacgttaa aggggttacc ttttccccta
     5341 gctcaggcaa caaaccaggt agccggaatg tgcaagcccc gttcatatcg tcggaccgag
     5401 gactcaccat cctggcgatt cggtgtgaca gccggaagag tacggcgcac aacaggaaaa
     5461 ggcgtttaat taacgcgagc gccgtttccg ttgtggtaat tgcggctatg cgctcgtgac
     5521 gaagccattt tcattcattg aggttctgat aagtgtcgtc gccgggcgtg gctgacctgg
     5581 caggtggagg caccaccgcc acaactcctt tcaattcatc atatagccgc tcaggctgta
     5641 acccttaatt aatacgaagt ttcgacatct ctatcggttt aaccgagaga tggtcgcgcg
     5701 tgtaaaaagg agacgttatg ttaatggcga acgataacgc atatgcagaa gaagatctca
     5761 ttttgagtga cttcataggg aagcgggaac gctggaccca aaagcgagag gaactttacg
     5821 cctcattagt tcgcaaaggc gtcaatatcg aaaccgcaca aagcggcgat atgacagtgg
     5881 taagcgttgg attgcacggt gtaagcgtgt cagcaatcaa tcatgagccg tacgttgcgt
     5941 tatccgagtc aatggtaagg ttagttaagt tcctgaagta cacagaagcg aataatgtaa
     6001 tcattggcaa aaagaatatc cctttttcct cggcttttta ctggatgatg aaagggcttg
     6061 atgcccggcg gacatcatgg cctaaaggga gctatatatc gatgttccgt ggcagcattg
     6121 gcagcaaaga aaagctcttc gaattcttgc cggaagaggc attcgatatt gtggaagggt
     6181 gcgatgtgat ggtgatgcct cgcctggtaa tgatgaatgg tgatttacag gcgcagacag
     6241 actggtttgc aactggtgtg gatattatcg cgactgactg ggaggctttc tgatgtacgg
     6301 gactaccaaa ttaagcactc aacaggccac caatatggag tggtctatcg ctaactgtaa
     6361 gatcgtgtca tgcaatgcca cgcgtactga tgtgggccat aaaagcacta taacaggcta
     6421 tctagtaatg accactaggg ggatgagggt tagatttcgc tggaaagcag agcaagtaac
     6481 gccattcaga gagccatcaa tgccgctgca ggttgagcag gattatctat gtttaccaga
     6541 aatagatgga ggaatgctgg tggagtctga tggtagtaag gctgattttg aagccaattt
     6601 gaagctggca caggccgtaa tcaatagtta tcgcggaaag tggatcgcaa aagtgaaaca
     6661 tgttgttcgt ttgtctcgct ggtagcgtta ggtatttaat attaattttc taatataatc
     6721 atctttttac cagtctatac aattttaaca ttctcattat catcccctcg gtcatttcct
     6781 gcccggggga ttaatggaac aaatcagcag cgcagaaatt gcggatatca tgatcagggc
     6841 tgactgctat ctgacagtca ctgaaattac cactctcgcc aaagaaaaat accctcatct
     6901 ccatgtttca cgagtaagtg taactaacat cattcgtcat tttgttcgtt caagcagggc
     6961 aatctgtgag ctggatgatc gtgtgtatcc acgaaaatac tggttacatg gtctgaacgg
     7021 atatcagttc aaggtgcgcg ggcgcacgcc tgaatacggt agcttactgg ttaaaaactg
     7081 tagcaggaaa tcagtcgaac aggcgcgaaa agagcaacga gagctggtgg acatggcgaa
     7141 taagctgtgg aatgccgccg ttaaaaagcg tggtgttgct ttatgagtct ggttaattgg
     7201 gaggagcacc gcgcccgctt tatagcgttg cgtgaagaaa aagggatcac tgtaaaggaa
     7261 tactgtgagg agcatggcct tagcttcaat acagccagaa agcatctcaa tatgaaaaaa
     7321 aatgaggtgc ggtcgcaggt aaagacaaca aaaaaaagca ccaaatcacc tgaaaaaaaa
     7381 cgggcgggaa gagcaacaaa aaagctggaa gagggtgcag aaaaaacaaa atttacagaa
     7441 attgacaatt cgcagggtgc aggttctttc cgaagtatgc caagaaagaa aacttcggat
     7501 catgaaagta ataccaataa taacaatgag ataaaagaat ctgtcaggag atcattgcgc
     7561 gaaaaaatgg cgaaaatgat ccccgaagaa gataaaagcg ccccaaaaat tgagggatct
     7621 tcagcaatga tccctggcgg tgctgtgcag cgtgccacat tgcccactac gaatgtggct
     7681 cgcgatatga tgaagaacgg cgcagaagaa cacctgcggt tagcaattca aatggcgcaa
     7741 gagcgcgcac ttcagtatca aagcattgtt gatcaggagg ccgaacgtct acaggcggaa
     7801 atagacgcct tgggcgataa agagccagaa ggaatgcacc ctgggcagcg ccttctcggt
     7861 ctaatcagtg acgcggccta ctacatgaat gattttatca gtaggttggc agctatatat
     7921 cagtccgaac aaaagctgcg acagggggat gaaaagcttc gtcagagcgc tcgccagcaa
     7981 gcattcaaag aggctgaggc cagagaaaaa ctcgaattag cacgcctaca ggccgaacag
     8041 cgtggcaaag agattgaata tcgcattggc gctgacgcac gcgcagccag agttatcgcc
     8101 gccgctatac gtatgcgtga tcgcgaggaa ctggatgata tcggtgttgc tgaatacatc
     8161 gagcgccagg gggtcagcgt cccggctatc ctcgcggcgc gcgcggcgaa agctatcact
     8221 cttctcgaac cacctgtttc tgacgtcaat gatgttgatg atgaacagct ggacaaagag
     8281 gcaagggagt ttgcaacgct tcaggctaac catccgcaat ggctggcaga gcgccgggcg
     8341 gatgtggcaa ccattgttga agaattgggg tgcggagatt acgaccgcaa tggcgagcgt
     8401 aaagctggag aattcgaggc caacgacgaa gagctggata ttgacccttc ggcaacggct
     8461 gaaatttacg gtgattatga tgcatctgat gcagggtatg acgccggaga tgatgatatc
     8521 gcgatagaac cgccggagga tgattaatgg ctggcgggcg caagattaaa tgcgtcacca
     8581 gcgatccacg ctggcgcgat atggtaatca aataccgcta caactttacc caggctgttg
     8641 tcgatatttt tgggatgttc ccctcccacc agcagcagca gatcattcag tcagtgcagg
     8701 aaacggggag taggactacc gtcacgtccg gacacggaac ggggaagtcg tcccttaccg
     8761 cgatgttgct gttgattttt atgatcctgt tccccgatgc ccgtgtcatc attgttgcta
     8821 acaagatagg ccaggtaaaa acaggcgtat tcaaatacgt taaacagtat tgggcgaatg
     8881 cagttaagcg tcatgggtgg ttgcagacct attttgtcct ctctgacacc atgttttatg
     8941 agcgctcccg taaggggatt tgggaagttc tctgcaaagg ttatcgactc ggcaacgaag
     9001 aagcgctggc gggggaacac gcggcacatt tgcttctaat tctggatgaa gcatctggta
     9061 tatctgataa ggcgatcggc gtaatgaccg gtgctcttac tgaagaagat aaccggatgt
     9121 tgatgctatc ccagccaaca aggccaagtg gttatttcta cgattcgcac cattctcaag
     9181 caaaaacccc tgacaacccg aaagggatct ggacagcaat tgttcttaac tcggaagaat
     9241 caccgttcgt cacaccacaa ttcatcaagc agaagcttct ggaatatggc gggcgcgatt
     9301 ctattgagta catggtcaag gtgcttggtc agttcccacg agaaattaac ggctatttgc
     9361 ttggtcgtga tgaatgcgat cgcgctgctc gccgcaaggt gttgttggag aaaaactggg
     9421 gatgggtggc aacggctgac gttggtaatg ggcgagataa gtcggtcctc aacatctgca
     9481 aggtatctgg tcacagggat aagcggcgcg ttgttaactt taaggtaatg gagatgcctg
     9541 gcactatgga cccattggcc tttgctgact tcatatataa cgaatgtaca ccggaaaaat
     9601 acccgaatat aacaatcgcg gttgatgctg atggttttgg ttctgatacg tgtgcgcagt
     9661 tggtgcgtcg tggtgcgaac ccggtacgta ttcggtgggg aaaacccatg tttgcgaaca
     9721 aagacaggga gcgatttgtt aatcagcgtg cctacgcaaa tattatggcg cgtgatgcca
     9781 taaagtcagg gcgtatgcgg atagacagtg accctaaaac ggcagagcaa gcgtcaaaaa
     9841 tccccttttt actgaatgaa gaagggaaaa tggcgatgat gcgcaaagag cacatgcggc
     9901 aaaagctaaa catcaaatcg cctgaccgat gggataccta ctgcttcact atgctggtgg
     9961 actatgtgcc tgctaatgaa gatattggtg ccgaaatggc aactttccga gatcaggttc
    10021 tggcggatat cgaaatgccg gatctggata tataaagccc cgcgatgcgg ggccggttgt
    10081 gattaccaga gcttcgcggc gtactgcgtt tgggggaatg tgtatccgta acgttcttcg
    10141 attgctttgc cgtatttttt acgagcttca ttcagtttaa tgccggtcag gtcgtctcca
    10201 gaggccatca ttacgtctaa caattccaga tccattttat ccagccttgc cattgcttca
    10261 cgtgatgtac gtatcttacg ctcaatgata tggcgaatag ccggatcggc atcctccagc
    10321 atcttagcgg atttctgctt aaggctttca ttgcgaatat cgatgttcct taagatgtac
    10381 gacttcgcaa cgtagcttgg ctggtgtttt tccaggtcac gaacacgata taagttaaaa
    10441 tcgccgagaa ggctgcaagg taccgattcg acgccagaat aaattgtaat gctccggcgt
    10501 tcaccaacat tattgacaac ggggatcgtt gtcttcggta gtcttgcgaa aaccaatgcg
    10561 ccatgtgcgc caactctaaa gcgagaaagg gcattgctgc cttcgatgtt tgttgtcagc
    10621 acaacatctg tatatgcctc tttacgcgcc cggtagatct cgccttcagg ctcagcttca
    10681 aagaagcgct gttcttcttc aagagcacct acgatttgag aaaaaaccct atttttcagg
    10741 cttttcttta tgattttgtc gtattttttg agcaattgtt cacgtgtaaa tgtacgtgtt
    10801 gttgtagcgc tttgcacaac aacatgcgtg ctttgattgg tgtcctcaat gatgaatggg
    10861 atcggagaat atacagactc aacaatgttc gacgctttgc cgtcggcatc gcggcacatg
    10921 tcaacgaagg ctttccattc agcaagacat ttttcagctt cttctaatat gttttggtca
    10981 tgcgttaatg caacatggcg gaattcctcg ccagcataac tggtatcatg gaaaacagag
    11041 tttgcgacca ccagctcttt ccaccgttca gcggtttgca ggaaccgttt gtactctttc
    11101 tcgatcgact tcttactcat tattcctact cgatttagtt ttctaacata
//
LOCUS       DAZFWP020000155        11126 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFWP020000155 DAZFWP020000000
VERSION     DAZFWP020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.155"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="ACHK0Z_RS20075"
                     /old_locus_tag="pgaptmp_004015"
     CDS             complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="ACHK0Z_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU4503949.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1249..1515
                     /gene="sdhE"
                     /locus_tag="ACHK0Z_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             1249..1515
                     /gene="sdhE"
                     /locus_tag="ACHK0Z_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU4503950.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            1496..1903
                     /gene="ygfX"
                     /locus_tag="ACHK0Z_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             1496..1903
                     /gene="ygfX"
                     /locus_tag="ACHK0Z_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU4503951.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="ACHK0Z_RS20090"
                     /old_locus_tag="pgaptmp_004018"
     CDS             complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="ACHK0Z_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU4503952.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2576..3472
                     /gene="xerD"
                     /locus_tag="ACHK0Z_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     CDS             2576..3472
                     /gene="xerD"
                     /locus_tag="ACHK0Z_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU4503953.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3497..4207
                     /gene="dsbC"
                     /locus_tag="ACHK0Z_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     CDS             3497..4207
                     /gene="dsbC"
                     /locus_tag="ACHK0Z_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU4503954.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            4213..5946
                     /gene="recJ"
                     /locus_tag="ACHK0Z_RS20105"
                     /old_locus_tag="pgaptmp_004021"
     CDS             4213..5946
                     /gene="recJ"
                     /locus_tag="ACHK0Z_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU4503955.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6037..7135
                     /gene="prfB"
                     /locus_tag="ACHK0Z_RS20110"
                     /old_locus_tag="pgaptmp_004022"
     CDS             join(6037..6111,6113..7135)
                     /gene="prfB"
                     /locus_tag="ACHK0Z_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU4503956.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7145..8662
                     /gene="lysS"
                     /locus_tag="ACHK0Z_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             7145..8662
                     /gene="lysS"
                     /locus_tag="ACHK0Z_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4503957.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8705..9253)
                     /gene="idi"
                     /locus_tag="ACHK0Z_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             complement(8705..9253)
                     /gene="idi"
                     /locus_tag="ACHK0Z_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU4503958.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            9308..9379
                     /locus_tag="ACHK0Z_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             9308..9379
                     /locus_tag="ACHK0Z_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503959.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(9376..9501)
                     /gene="yqfG"
                     /locus_tag="ACHK0Z_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             complement(9376..9501)
                     /gene="yqfG"
                     /locus_tag="ACHK0Z_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU4503960.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="ACHK0Z_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="ACHK0Z_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU4503961.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         2817 a         2780 c         2969 g         2560 t
ORIGIN      
        1 taaatgcctg atgcgataaa gagatttact cttcgagcga atacggcagc ggctcgatat
       61 gcagtgtatt cgcatcgtcg cgtacgcgga agatgctatc cggttccata tcgttattca
      121 tgacgacctg taccacgacc tgaccatctt ccagttttac cgcagccagc accgtaccgg
      181 tacgacgcca gttctcgccc attttcagct ctaagtcttc accagcttcc ggcagtcggc
      241 tggcgctacc tgccagcaac cagagcgcac gtttattggc accacggaat tttgctcgcg
      301 ccaccatctc ttgtccggta tagcagcctt ttttaaagct gatgccgccc agcgcctgga
      361 ggttggtcgc ctgtgggata aactgcccgc tgttggcggc atcaatcacc gggaaacccg
      421 cttcaatgtt taatgccagc cactgttggc tattgttcag ttccgcttca ccgcgcagtt
      481 tatcggtcag catattagca gtagcttcat cggttacgat caggaaacgt tctgccgggt
      541 gttcaaacca tagcaaagtg gtcgcgcctt ctttaactac ctgtttttct ttcgaaggca
      601 gttcactaaa gagatttgcc agcgcggcgc gcgcctgaaa accggcaaca ccaagcagca
      661 cacgctcgtc gtctggcgcg atggtcactt tagagaatac cgcatatttt ttcagttcag
      721 tcagctgcgg ttcacgcacg ctgcgccgtt caatccatgc aaagccatcg ccgtcgcgga
      781 acagacgtaa attgctccac attttacctt tggcgtcgca atgggcggcg agcaggtgct
      841 gatcttctgc catctggctg acatctgctg tcacctgacc ctgcatatat ttttcgctgt
      901 ccgcgccagt aatggtggca agcgcccagt catcaagcgt catcagcgtc agcggtaaac
      961 gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa agccataata gagtcctgag
     1021 ttgcttaacg gcgtattaat gactaatggt aaaagagcta tgaggcaatg caagtgcttt
     1081 cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagcc attgaattgt
     1141 tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat tcccgtaaaa gaaggcgaaa
     1201 aacacgttac aatagccggg tactggattt tcgtgagaca ggaagaagat ggacattaac
     1261 aacaaagccc gcattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc
     1321 atgccgtttt tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg catctttatt
     1381 cgtctgctgg aatgtgacga tccggacctg tttaactggc tgatgaatca cggtaaaccg
     1441 gccgatgcag aactggaaat gatggtccga ctcatccaga cacggaaccg ggaacgtggt
     1501 cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag tggctttcct tgctgattca
     1561 tgggctggtt gccgctgtta ttttactcat gccctggcca ctcagttaca ccccgttatg
     1621 gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc agccagcggc gtattaatgc
     1681 tcgccagggg gaaattcgct tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg
     1741 gtgcatcgtc aaagcaccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga
     1801 tggcggtaaa cggcaacatt tatggctggc agccgacagc atggacgaag ccgaatggcg
     1861 ggatttacgg cggattttgt tgcaacaaga gacgcaaaga taaagatgac ggcaaagcat
     1921 atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc catttcgttg aggatttgct
     1981 cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca
     2041 gacccacgaa cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt
     2101 ccgttggcca gtagccgacg aacttcacgc ctttggtcga gagtttgtca tgcagcatac
     2161 cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa
     2221 caattttacc ttcaaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttcct
     2281 ggatttcacc aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta
     2341 atttcgggga gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc
     2401 ggattttttc tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat
     2461 tcataacgta agcttctcga ttctgctgtg gcttttgcag ctagtgtatc agaatcgctt
     2521 tagtcacggc acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa
     2581 acaggatctg gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc
     2641 tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca
     2701 ccgcgggttg acgctggcga cggcgcaaag tgacgatttg caggcattac tggcagaacg
     2761 gctggagggc gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt
     2821 gttccagtat ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc atctcgcttc
     2881 accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggtcg aacgtttatt
     2941 acaggcacca ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt
     3001 gtatgctacc gggctgcgtg tctctgaact ggtcggactg acaatgagtg atatcagcct
     3061 gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg taccgttagg
     3121 tgaagaggcg gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgctgaa
     3181 tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac
     3241 cttctggcac cgcattaaac attatgctgt gctggcgggt atcgacagcg aaaagctgtc
     3301 accgcatgta ttgcgtcacg cttttgccac ccatttatta aatcatggtg cggatttacg
     3361 cgtggtgcag atgctactgg gccacagcga cctctccacc acgcaaattt atacgcatgt
     3421 cgctaccgag cgtctgcggc aacttcatca acagcatcac ccgcgggcgt gatgtctgaa
     3481 aagaacggga agatttatga agaaaggttt tatgttgttt actttgttag cggcgttttc
     3541 aggttttgct caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa
     3601 aagcagcgat attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg
     3661 cgtgttgtac atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag
     3721 cggcacggct ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga
     3781 aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga
     3841 tattacctgt ggttactgcc acaaactgca tgagcaaatg gcagactata acgcgctggg
     3901 gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga
     3961 aatgaaagct atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg
     4021 taaaagtgcc gcaccagcca gctgcgacgt ggatattgcc gaccattacg cacttggcgt
     4081 ccagcttggc gttagcggta ctccggcagt tgtgctgagc aatggcacac ttgttccggg
     4141 ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg
     4201 taaataattc gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga tgaaacggca
     4261 gacttgcccg ctgaattgcc tcccttgctg cgccgtttat acgccagccg gggcgtgcgc
     4321 agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc cctggcagca actgagcggt
     4381 gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg
     4441 gtcggtgatt tcgacgccga cggcgcgacc agcacggctc taagcgtgct ggcgatgcgc
     4501 tcgcttggtt gcagcaatat cgactacctg gtaccaaacc gtttcgaaga cggttacggc
     4561 ttaagcccgg aagtggtcga tcaggcccat gcccgtggcg cgcagttaat tgtcacggtg
     4621 gataacggta tttcctccca tgcgggggtt gtacacgctc gctcgttggg cattccggtt
     4681 attgttaccg atcaccattt gccgggcgaa acattacccg cagcggaagc gatcattaac
     4741 cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg tgtggcgttt
     4801 tatctgatgc tggcgctgcg cacctttttg cgcgatcagg gctggtttga tgagcgtggc
     4861 atcgcaattc ctaacctggc agaactgctg gatctggtcg cgctgggaac agtggcggac
     4921 gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt
     4981 gccggaaagt gccgtccagg gattaaagcg ctgctggaag tggcaaaccg tgatgcacaa
     5041 aaactcgccg ccagcgattt aggttttgcg ctggggccac gtctcaatgc tgccggacga
     5101 ctggacgata tgtccgtcgg tgtggcgctc ttgctgtgcg acaacatcgg cgaagcgcgc
     5161 gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa aagagatcga acaaggaatg
     5221 caagttgaag ccctgaccct gtgcgagaaa ctggagcgaa gtcgcgacac gctacccggc
     5281 gggctggcaa tgtatcaccc cgaatggcat cagggcgttg tcggtattct ggcttcgcgc
     5341 atcaaagagc gttttcaccg tccggttatc gcctttgcgc cagcaggtga tggtacgctg
     5401 aaaggttcag gtcgctccat tcaggggctg catatgcgtg atgcactgga gcgattagac
     5461 acactctacc ctggcatgat gctgaagttt ggcggtcatg cgatggcggc gggtttgtcg
     5521 ctggaagagg ataaattcga actctttcaa caacggtttg gcgaactggt tactgagtgg
     5581 ctggaccctt cgctattgca aggcgaagtg gtgtcagacg gtccgttaag cccggccgaa
     5641 atgaccatgg aagtggcgca gctgctgcgc gatgctggcc cgtgggggca gatgttcccg
     5701 gagccgctgt ttgacggtca tttccgtctg ctgcaacagc ggctggtggg cgaacgtcat
     5761 ttgaaggtga tggtcgaacc ggtcggcggc ggtccactgc tggatggtat tgcttttaat
     5821 gtcgataccg ctctctggcc ggataacggc gtgcgcgaag tgcaactggc ttacaagctc
     5881 gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca
     5941 atttagcgtc atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca
     6001 ccgcattttg actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat
     6061 aatcgcattc aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga
     6121 cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa
     6181 cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga
     6241 caccctcgac caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt
     6301 agaagctgac gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga
     6361 aaaactggcg cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg
     6421 ctacctcgat attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct
     6481 tgagcgtatg tatctgcgct gggcagaatc gcgtggtttc aaaactgaaa tcatcgaaga
     6541 gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc
     6601 ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaga gcccgtttga
     6661 ctccggcggt cgtcgccaca cgtcgttcag ctctgcgttt gtttacccgg aagttgatga
     6721 tgatattgat atcgaaatca acccggcgga tttgcgcatt gacgtttatc gcgcgtccgg
     6781 cgcgggcggt cagcacgtta accgtaccga atctgcggtg cgtattaccc acatcccgac
     6841 cgggatcgtg acccagtgcc agaacgaccg ttcccagcac aagaacaaag accaggccat
     6901 gaagcagatg aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca
     6961 ggcgatggaa gataacaaat ccgacatcgg ctggggcagc cagattcgtt cttatgtcct
     7021 tgatgactcc cgcattaaag atctgcgtac cggggtagaa acccgcaaca cgcaggccgt
     7081 gctggacggc agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac
     7141 caacatgtct gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact
     7201 gaaaacgcgt cgtgagaagc tggcgaacct gcgcgagcag gggattgcct tcccgaacga
     7261 tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa ttcgacggta aagagaacga
     7321 agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgccgtat
     7381 tatgggtaaa gcgtctttcg ttaccctgca ggatgtgggt ggccgcattc agctgtacgt
     7441 tgcccgtgac gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg
     7501 cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac tgtctatcca
     7561 ctgtaccgag ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg
     7621 cttgcaggat caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga
     7681 atcccgtaac acctttaaag tacgctcgca gatcctctct ggtattcgcc agttcatggt
     7741 gaaccgcggc tttatggaag ttgaaacgcc gatgatgcag gtgataccag gcggtgccgc
     7801 tgcgcgtccg tttatcaccc accataacgc gctggatctc gacatgtacc tgcgtatcgc
     7861 gccggaactg tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa
     7921 ccgtaacttc cgtaacgaag gtatttccgt acgtcataac ccagagttca ccatgatgga
     7981 actctacatg gcttacgcag attacaaaga tctgatcgag ctgaccgaat cgctgttccg
     8041 tactctggca caggatattc tcggtaagac ggaagtgacc tacggcgacg tgacgctgga
     8101 cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga
     8161 aaccgacatg gcggatctgg ataacttcga ctctgcgaaa gcgattgctg aatctatcgg
     8221 tatccacgtt gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt
     8281 ggcagaagca catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc
     8341 gctggcgcgt cgtaacgaca ttaacccgga aatcacagac cgctttgagt tcttcatcgg
     8401 tggtcgtgaa atcggtaacg gctttagcga gctgaatgac gcggaagatc aggcgcaacg
     8461 cttcctggat caggttgccg cgaaagacgc aggtgacgac gaagcgatgt tctacgatga
     8521 agattacgtt accgcactgg aacatggctt accgccgaca gcaggtctgg ggattggcat
     8581 cgaccgtatg gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc
     8641 ggcgatgcgt ccggtaaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg
     8701 ttttttaatt gtgctgcgcg aaagcagaca acaattttct tgcttcacta ttggctgcct
     8761 gcatcaccat ccacggactg aacgcccacg gtgtggcatc aataccgtgt aatacatctg
     8821 ctaaatcaca ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg
     8881 tgcgtgcggc aaataccgga cacacttcat tttccacaat gccattcgga tcggtggcgc
     8941 gatagcgaaa gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac
     9001 ggcaacggcg gatcaccgcg tcttcgttgc tttctcccag ttgtgggtgc ccacaaaccg
     9061 agttagtcca cacgccaggc cacgcttttt tgctcagtgc gcggcgggta actaataatt
     9121 gtcctttggc attaaacagc caactggaga acgcgagatg taagcgggtg tctgccgtgt
     9181 gtgcggcata cttttccagc gtaccagtgg gaactccctg tgcattcaat aaaatgacgt
     9241 gttccgtttg cataatttct cacatgtaat tctgatcact catcattata gataattgaa
     9301 gagtgaaatg attaaccaag tgagcgttta tcgacaaccg cccgttttga gcggatgccg
     9361 acaggtaaaa accatttaaa aaagtagcat catatatttg aagcgacttt caatggccat
     9421 tgcaacacag tcagaaatga caggaataga gagagtaaaa agcaacagca gactgaatat
     9481 agcgcgcatt aaaaaattca tattattctc catgctcatt tttcttcagc ttgcgcatca
     9541 gaagccacat acgtactgtc ctgactttta aatctttatc agagacggat tccttcacca
     9601 ggtcagcatg atgctgatag ccattaaaga agacattcag cacaacggcg ctaagggttg
     9661 cgagcataat gccgctatgc agcaacggtt gcagtacggc cggtaactta gaaaagaaat
     9721 cgtgagagag cgtcggagtc atgccaacgc cgagactgat cgccacaata tagaggttgt
     9781 aacggttggt ggtgtagtta cagcgcgaca gaattcgaat ccctgtcgcc agtaccatgc
     9841 cgaacatcac cagcccagcg ccgcccagca caaattgcgg aatggaggct accagcaccg
     9901 ccatttttgg caccatgcca aacaggatta atataattcc cgaagaaata cacacccagc
     9961 ggctatgaac gcgcgtcacg ctaaccaggc caacgttttg agaaaatgac gtgtggggga
    10021 agctgttaaa cgtgccgcct atcattgtcc ctacgccatc gacacgcagc ccgcgaataa
    10081 tatcgtgtga agagagttta cgaccgacta tttcacccag tgccaggaac atccccatcg
    10141 actcgataaa cacgatgatt aacacggcag tcatggtcag aatggaaacg ggatcgaaaa
    10201 tcggcatacc aaacgacatc ggggtgacaa tcgcaaacca cgaggcatca tgtagcccgg
    10261 ataaattgac ttcattcatc atccacgaaa gtaaaaagcc aaatacaatc cccagtaata
    10321 cggcgacgtt ggacataaat cctttcgcat agcgagtaat gagcaagata aaaattaaga
    10381 cggcaaagga gatacctaaa taaacgggat taccatattg cggattccct ttacctccgg
    10441 cggcccagtc aatacccacc tgaatgatgc taagcccaat agaagtaata accacaccgg
    10501 taaccagtgg cgggaataaa ggcatcaagc gaccaataag tggcgctaat aatgtggtga
    10561 taaaacccgc ggcgatagtg gcaccaaata tccccagcag gccgatatcc gggttcatcc
    10621 caatggctat cattggtgtt acagcagcaa aggtcaccga cataatcacc ggcaggcgga
    10681 tccccataaa gcggccgata ccgatacatt gcaataatgt gacgatcccg cagcaaaaga
    10741 gatccgagct aatgagcatc gcaatagctt cttttgagag gcccagtcgg tcaccaatca
    10801 taagaggaac agcgactgca cctgcgtaca tgacaaggac gtgttgcaga ccgagtatga
    10861 tcagctttcc tggtgataat atgcggtcaa cctcatcagt tgggcggtct tgcccagaag
    10921 atgagggaag ttgggaatct atggcgctca tcagtgtttc tcctttcgcc cgccagagta
    10981 aaaagcaacc gtttacgtta atgaaactgt ctaaacacat gctcgtggag tgccagtttt
    11041 tgcgaggaag gggagttaga gacaggaatt gcggaggcaa tcacataatc gtgcttaaac
    11101 cacgaaaatg agaagagaac ctgaca
//
LOCUS       DAZFWP020000156        11090 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFWP020000156 DAZFWP020000000
VERSION     DAZFWP020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.156"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            135..851
                     /gene="nanC"
                     /locus_tag="ACHK0Z_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             135..851
                     /gene="nanC"
                     /locus_tag="ACHK0Z_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU4503962.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            871..1977
                     /gene="nanM"
                     /locus_tag="ACHK0Z_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             871..1977
                     /gene="nanM"
                     /locus_tag="ACHK0Z_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4503963.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2042..3022
                     /gene="nanS"
                     /locus_tag="ACHK0Z_RS20150"
                     /old_locus_tag="pgaptmp_004030"
     CDS             2042..3022
                     /gene="nanS"
                     /locus_tag="ACHK0Z_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4503964.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMIEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWGEK"
     gene            complement(3090..3152)
                     /locus_tag="ACHK0Z_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /pseudo
     CDS             complement(3090..3152)
                     /locus_tag="ACHK0Z_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3234..3377
                     /locus_tag="ACHK0Z_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /pseudo
     CDS             3234..3377
                     /locus_tag="ACHK0Z_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4177..4548)
                     /locus_tag="ACHK0Z_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             complement(4177..4548)
                     /locus_tag="ACHK0Z_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4503965.1"
                     /translation="MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFS
                     NETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDCSVMEKSPFITSIDILRARKASG
                     LMKRNSHKTVTRVTAGRLQEL"
     gene            complement(4587..4952)
                     /locus_tag="ACHK0Z_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             complement(4587..4952)
                     /locus_tag="ACHK0Z_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4503966.1"
                     /translation="MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLS
                     DRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGELNPRHPHCVTLYHNGLTCEADT
                     LGSCGYVYIAIYPEQTEPQ"
     gene            complement(4978..5199)
                     /locus_tag="ACHK0Z_RS20175"
                     /old_locus_tag="pgaptmp_004035"
     CDS             complement(4978..5199)
                     /locus_tag="ACHK0Z_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4503967.1"
                     /translation="MKIISKRQAMALYRQHPGSRLFRFCTGKYKWSGSICHYAGREVQ
                     DIRGVLAVFAERRQDRNGPYVILRSITLN"
     gene            complement(5196..5738)
                     /gene="radC"
                     /locus_tag="ACHK0Z_RS20180"
                     /old_locus_tag="pgaptmp_004036"
     CDS             complement(5196..5738)
                     /gene="radC"
                     /locus_tag="ACHK0Z_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4503968.1"
                     /translation="MNTTISPLLSQPELFPVSVMAQHGCLLPTTSGLTPYAQRTIRRA
                     MNLLDKYLRQPGIAFTSSTAARDWLRLHLARQEREVFMVLYLDNQHRLLESETLFAGS
                     VNHVQVHSREVVKSALRFNAAAVVLAHNHPSGDPEPSQCDRNITGRLKEALELVDVKT
                     LDHLVVGRDGIVSFAERGWL"
     gene            complement(5751..6194)
                     /locus_tag="ACHK0Z_RS20185"
                     /old_locus_tag="pgaptmp_004037"
     CDS             complement(5751..6194)
                     /locus_tag="ACHK0Z_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4503969.1"
                     /translation="MNTQNLEFAEQAAITASAVPDELRIGFWPQHFGSVPQWITLESC
                     IFDWMDRLCTDYHGGIWSFLTLSNGGAFMAPESEHDEKWALFNSMNGNGAELTGEAAG
                     IVACLMTYSHHACRTECDAMTEHYYRLRDFALNHPECSAIMHLID"
     gene            complement(6225..7046)
                     /locus_tag="ACHK0Z_RS20190"
                     /old_locus_tag="pgaptmp_004038"
     CDS             complement(6225..7046)
                     /locus_tag="ACHK0Z_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4503970.1"
                     /translation="MRLASRFGRINQIRRDRPLTHEELMSHVPSVFGSDKHESRSDRY
                     TYIPTITILESLQREGFEPFFACQTKVRDQSKREHTKHMLRLHRAGQLTGHQVPEIIL
                     LNSHDGSSSYQMLPGLFRGVCTNGLVCGQSFGEVRVPHKGNVVEKVIEGAYEVLGVFD
                     RVEEKRDAMQSLALPAPARHALANAALEYRFGEDHQPVTVSQLLTPRRREDYSDDLWT
                     VYQRVQENLMKGGLSGRTAQGKSSRTRAVTGIDGDVKLNRALWVMAENMLEFFGR"
     gene            complement(7166..7639)
                     /locus_tag="ACHK0Z_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             complement(7166..7639)
                     /locus_tag="ACHK0Z_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503971.1"
                     /translation="MRLPYVIVTLAMLASVSTAQAIPNMWTSGFGMGVTEYIITSPDK
                     VVFNLNCTGNPDAQNILQHGVDLTLPDGISVSSHDDGTEITVVMDNSQYPLPSFLGWR
                     NGDNAWVSFIDALSQAANFDVYVNDKKVGTFSPGLKNTQKELSDLSECRTTHYSD"
     gene            complement(7711..8163)
                     /locus_tag="ACHK0Z_RS20200"
                     /old_locus_tag="pgaptmp_004040"
     CDS             complement(7711..8163)
                     /locus_tag="ACHK0Z_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503972.1"
                     /translation="MKKLKLLSLLPVMFFPLLIAGNAHAARPYSGFLPAAVNNKSGNI
                     FWCDAHRQVRKLCTATEVDSMGWFIVVRSKPSRDCPGGIWGWITPSKDDVATGYTITP
                     GKEFNLTMKDMCSPDMKVTFRPNKKDPRYDDLVGIYAGKEAFRYKQIK"
     gene            complement(8199..8915)
                     /locus_tag="ACHK0Z_RS20205"
                     /old_locus_tag="pgaptmp_004041"
     CDS             complement(8199..8915)
                     /locus_tag="ACHK0Z_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4503973.1"
                     /translation="MTLQERRHDRLAVRLSLIISRLVSGETLFIRKLATEFGVSERTL
                     RRDFRERLMYLDLDYYQGQCSLRTDIHSTRQEIDILTFAHRTGLAGVFPGFDRRLVSS
                     LLDCDDRPCLVWHQDRPHPPAGKLSFYRLVRAISGCQRITILTEGERHEAVAPYRLIA
                     LDGYWYLVAEHNRQLRVFRLGEIHLVRPSRETFIRSELLYQLSEDKHFISALPHFRFI
                     QQSLNSFSPSESRGQSGQTD"
     gene            complement(9159..10034)
                     /locus_tag="ACHK0Z_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             complement(9159..10034)
                     /locus_tag="ACHK0Z_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4503974.1"
                     /translation="MNKPDGLQAFEQPLASLPCTLRQLILERIQNLTHYEPVIGIMGK
                     SGTGKSSLCNELFQGEVSPVSDVNACTRDVLRFRLRSGRHSLVIVDLPGVGENGRRDH
                     EYRALYRRMLPELDLVLWVIKADDRALTLDEQFWLGVMQPYRQKVLFVINQADKIEPC
                     YEWDTLTSKPSTHQQGNLKAKQAAIMAMFKPHHPVCAVSASTGWGIEDMVAAMMRCLP
                     DRATSPVVTQLHGRLCTEPVKSQARDSFGDAVGRVFDTAESSSLLPAPLKKVIRTVRD
                     AVVSVARAVWDWIFF"
     gene            complement(10119..11003)
                     /locus_tag="ACHK0Z_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             complement(10119..11003)
                     /locus_tag="ACHK0Z_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4503975.1"
                     /translation="MVKLVGNDSSKIKYLENKLIENGYHFYSGGVDKDYREYQLRVFN
                     YLVSQNVSEQNINSFFAEVDNSYTRGFPSESELDWYRNDPRASLWLSCELYEKLKEET
                     PKYNIDFLSPEALQPDHNVRIEAIRHCMDEWPMYFTTPAEFIKDKSIEWAELLDQHDL
                     FRSVRSSKVDVCSWLRDYLRGNTSIGLKRICGNSSEEIMSWCYASYFIWRKNNLHSPD
                     SVELFIRKFKSAWSTQKNRNKNKEEKKLVTMSVNISQQAHDMLRDMSMKDSMSNNAII
                     ESAILRLYNIKNSKVRSK"
BASE COUNT         3065 a         2619 c         2583 g         2823 t
ORIGIN      
        1 caaaaaaata taaaatacag ctaccggttg ccaaagacac tataagcctg gcaaaaaaat
       61 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
      121 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
      181 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      241 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      301 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
      361 atgatgttca ggttgaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc
      421 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
      481 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      541 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      601 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
      661 aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc
      721 tacaatacca tatgacgccc gatattacgc catacataga atatgactac ctggaccgtc
      781 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
      841 ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc
      901 atgatggctt catttgccgc aaacgcgtct gtattacctg aaactcctgt gccatttaaa
      961 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg
     1021 gcatggtaca agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc
     1081 cctggtggac caagagatca agcaacctcg gcatttattg atggcaatct gtatgtgttt
     1141 ggcggcattg gcaaaaatag caagggcttg actcaggtat ttaatgacgt acacaaatac
     1201 aaccccaaaa ccaatagttg ggttaaattg atgtcgcatg caccgatggg catggcgggt
     1261 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     1321 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcagcaac tatagacaag
     1381 atcaatgccc attattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     1441 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatctccctg gtacggaacg
     1501 gctggtgcgg cgcttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     1561 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     1621 aataagcttg ctcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
     1681 aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag
     1741 aacggtaaga actatgcgca tgaaggccta aaaaaatcat atagcactga tattcatctt
     1801 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     1861 tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg
     1921 gtgacggatt cagttttgat ctctgtgaag gataataaag tcacagtaca aaattaacgc
     1981 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac gcgaaattta
     2041 tatgaacgca ataatatcgc ccgattatta ctatgttctt accgttgctg gtcagtctaa
     2101 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcatcccag
     2161 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa
     2221 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     2281 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     2341 acggaaatta ctacccttta ttcctgataa tgcaggggtt ctcatcgttc catgttgccg
     2401 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     2461 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
     2521 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
     2581 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatgat
     2641 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     2701 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga
     2761 agttatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     2821 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     2881 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     2941 tcatttcagc gcggcagccc gtcgggggat tatttctgac aggtttgtag aagcaatttt
     3001 gcagttttgg ggcgaaaagt gagcgttcat taatttgatt tattcgcatc catcaacgct
     3061 tttaactcct ccggcgtaaa cggtggttag cgtcggtgca gcatcgcatg gcaattaggg
     3121 tacacaggca gcaaaaaaac aaatgagatc aaagtaatat atcttcactt gcaggcttga
     3181 acagccgaaa atttgcgagc atgatcgcaa ttatttcact gccagggata tcaatggctt
     3241 ccagtaaatc gctacagcag gcaattgcca atataaaaat ctggcacaag ggtgaacagc
     3301 gcgcgccgca taagccattg ttattgctat acgtattagc gggatacctg aatggacatc
     3361 cacgcctttt cgattattgt agtggaacag tctacgagga tgcagtttga agtgagtaag
     3421 taaaggttgc tggaaacgat caatgccgcc atacaatgat atttgaggat caccaatgag
     3481 taataaaaac tacgaaagtc accgcaaagc aatagttagt aaaggtatac cacccactct
     3541 attaaatagg ctcaccaatt cggatgttca ggtgatcaat accttcttaa cacgagtgag
     3601 caaactggaa ctatctcaac aagagaaaga ctggatcata aaaatcatct ctatggttta
     3661 gagaatttca gccttgtagc ctttcaaggg ttttgtgtgg gtacaaacat tatcgaatcg
     3721 ggatatagcc gcagctgtag ctgtatccac atcagtaagt tgatgtaggt tgacttaggt
     3781 tgagcgaaaa atagcgaaaa gccttgtgtg gcgcggattt cagtcataaa aaaacgtccg
     3841 ttgacgtcta ttgatgttcc gatggtgcga aggtgcgaag gtgcgaaggc cggactcgca
     3901 cattacaatt aactccttga attaaaataa ataaaaacaa actaatttta tgcgtaccaa
     3961 cgcagatacc aacacattaa ttcattggat tcgggactcg aatcagggca aagccaagtg
     4021 aacaaaattc ccctatgagt agatgataca gataaagatg agaagctggc tttagccttc
     4081 gcgaactcct catcatctgg ttactgttag ctttccctac attggacagc attgtatcaa
     4141 tgttgtcgat atcaacatta attgacttcc catagcttat agctcctgaa gtcgacctgc
     4201 cgttaccctg gtaacagttt tgtgactgtt gcgtttcatc agaccggagg ctttccgggc
     4261 acgtagaata tcgatactgg tgatgaacgg tgacttctcc atgacagagc aatcattaca
     4321 gtcaattctt accagttcat atttttcaac aagaaagttc accgcatcgc acagcgatac
     4381 tccagcatca atgtgttcca gtatggtggt ttcattactg aacggtgtgt cattcaacgt
     4441 cagaccatag tgctgcgaca acagacaagt cagtaaccgc tgccagattt caacgggtga
     4501 caggcagggc tgagtcgccc gtgtcgggtg tgatgatata gtttgcatga tgtttgctct
     4561 gtgagtttaa gaggacaagc ggggatttac tgcggctcag tttgctcagg atagatagca
     4621 atgtagacat aaccacagga gcctagtgta tcggcttcac aggtgagtcc attgtggtaa
     4681 agcgtgacgc agtgtgggtg gcgagggtta agctctccac tggtcagcat cagctcaagt
     4741 tgcttcatca ttaacggaaa tgcctggtca aggcgcattg caacatcgac ggcaaatgcc
     4801 ccgctaaatc ctgcgcggtc agagagaaaa tgcaatcggt tgccctcctg taccaggcgg
     4861 gcaccaaaac agggggtaat agcacattta agcccccact gctggcaggc gggattctcc
     4921 ggctttgtac cggattctga gtgattgttc attattgttc ctgattaaag ggaaggatta
     4981 attaagagta atgcttcgaa gaatgacata gggaccattg cggtcttgac gacgttcagc
     5041 aaatacagcc agaacgccgc gtatgtcctg aacttcccgg ccagcataat ggcagatact
     5101 gccggaccat ttatatttgc cggtacagaa gcgaaacagc cgagagcctg gatgctgtcg
     5161 gtacaaagcc attgcctgac gtttactgat gattttcata accaacctcg ctcagcgaac
     5221 gacacaatgc catcccgacc gacaacaaga tgatcgagcg ttttcacatc aaccagttct
     5281 aacgcttctt taagcctgcc cgtaatgttg cgatcgcatt ggctgggctc cggatcaccg
     5341 gagggatggt tatgcgccag cacgacagct gcggcattga aacgaagcgc cgacttcact
     5401 acctcacggg aatgaacctg cacatggttt accgaaccgg cgaacagcgt ttcactctcc
     5461 agtaaacgat gctgattatc gaggtagagc accataaaca cctcccgctc ctgtctggcc
     5521 aggtgtaacc ggagccagtc acgggctgca gtgcttgagg taaaggctat accgggctga
     5581 cgcagatatt tatccagcag gttcatagcg cggcgaatgg tccgttgggc gtagggtgtc
     5641 agtccggaag tcgtagggag taaacagcca tgctgagcca tgaccgacac cggaaaaagc
     5701 tcaggctgcg ataacagtgg ggatattgtg gtattcatat tcggtatgac tcagtcgatg
     5761 agatgcataa ttgcgctgca ctctggatgg ttcaacgcga agtctcgtaa gcgataatag
     5821 tgctcagtca tcgcgtcaca ctcggtgcga caagcgtggt ggctgtatgt catcagacag
     5881 gcgactatcc ctgcggcttc gccagtaagt tccgcgccgt tgccgttcat actgttgaac
     5941 aatgcccact tctcatcgtg ctctgactca ggagccataa aagcgccgcc attgctgagc
     6001 gtcaaaaagc tccagatacc gccgtgataa tcggtacaca ggcggtccat ccagtcaaag
     6061 atacaggact ccagggttat ccattgcgga acggagccaa agtgctgcgg ccagaagccg
     6121 atgcgtaact cgtcagggac cgctgaggca gttattgctg cctgctcagc gaactcaaga
     6181 ttttgtgtat tcattaaact atctccttat gcaggaactg ttgtttaacg cccgaaaaac
     6241 tcgagcatgt tttctgccat cacccacagg gcgcgattga gcttcacatc gccatcaata
     6301 ccggtaacag cacgggtgcg gctgcttttc ccctgagcgg ttcgccctga cagaccacct
     6361 ttcatcaggt tctcctgcac gcgctggtag acggtccaca ggtcatcgct gtagtcctcc
     6421 cggcgacgtg gggtcagcaa ctgcgatacc gtgaccggct ggtggtcctc accgaagcga
     6481 tattccaacg cagcatttgc cagcgcgtga cgggccgggg ctggcagcgc cagtgactgc
     6541 attgcatcgc gcttctcctc cacccggtca aacaccccga gcacttcgta agccccttca
     6601 atcaccttct caacgacatt gcctttatgt ggcacccgta cctctccgaa cgactgaccg
     6661 cagaccaggc cgttggtaca aacgccacga aacagccccg gcagcatctg gtagcttgat
     6721 gagccgtcgt ggctgttaag cagaatgatt tccggtacct gatgcccggt aagttgaccg
     6781 gcacgatgca ggcgaagcat gtgtttggtg tgttcccgct tgctctggtc acggaccttt
     6841 gtctggcagg caaagaacgg ctcaaagcct tcacgctgca ggctttcgag gatggtgatg
     6901 gtcgggatgt aggtataacg gtctgaacga gattcatgct tgtcactccc gaacacgctg
     6961 ggtacatgac tcatcagctc ttcatgggtc agaggccggt cacggcgtat ctggtttata
     7021 cgaccaaagc ggctggctaa tcgcatagct aactccttat ctgataatga aataaaaaca
     7081 aaaaggccat gttcccgaag gagcatggcc tgttgctatc tggtgttggt atgttgtggg
     7141 gtggagaggt ctgaaatgta agtaactaat cgctgtagtg tgtggtccgg cactcactga
     7201 ggtcagacag ctctttttgc gtattcttca gccccggact gaaggtgccg acttttttat
     7261 cgttgacgta aacatcaaaa ttcgcagcct ggctcagggc gtcaataaat gacacccagg
     7321 cattgtcacc gtttcgccag ccgaggaatg acggtaacgg atactgactg ttatccatga
     7381 cgaccgttat ttccgtcccg tcgtcatgag agcttactga tattccatca ggcagggtga
     7441 gatcaacacc gtgttgcaga atattctgcg catccggatt gccagtacag ttcagattaa
     7501 acaccacctt atccgggctg gtaatgatat attcggtcac acccatccca aaaccactcg
     7561 tccacatatt gggaatagcc tgtgctgttg atacagaagc cagcatagct aatgtaacta
     7621 ttacataagg cagtcgcata atgttgtcct caaatcatgt aataaagctt ttgttgttta
     7681 aaacaataaa gtaacgaaaa aacagttgga ttattttatt tgtttgtagc gaaacgcttc
     7741 tttccctgca taaatcccga cgagatcgtc ataacgaggg tcctttttat tcgggcggaa
     7801 cgttactttc atatcgggac tgcacatatc cttcatggtg aggttgaatt ctttacctgg
     7861 cgtaatggta taacctgttg ccacatcgtc tttactggga gtgatccacc cccatattcc
     7921 acccggacaa tctctggaag gtttcgagcg aacaacaata aaccatccca ttgaatccac
     7981 ttccgtcgct gtacacagtt ttctgacctg tcggtgggca tcacaccaga atatattccc
     8041 acttttatta ttcactgccg caggcaggaa cccgctataa ggacgtgctg cgtgagcatt
     8101 acctgcgata agtaagggaa aaaacatgac tggcagtaaa gacaataatt tcaatttttt
     8161 cattagatta accttacatt caattgtgga gaccatctct aatcagtttg tccggattgc
     8221 ccccgacttt ctgatggaga aaatgaattc agcgactgct gaataaaacg gaaatgaggt
     8281 aaggcagaga taaaatgttt gtcctccgac agctgataga gaagctcact gcgaataaat
     8341 gtttcccgcg atggccttac cagatgaatc tcacccagcc ggaagacacg taattgcctg
     8401 ttatgttcag cgacaagata ccagtaaccg tccagagcga ttagccggta aggtgcaacg
     8461 gcctcatgac gctccccttc agtcagaatg gtgatgcgct gacatcccga aatagctctg
     8521 accagcctgt agaaggagag ctttcctgcc gggggatgag gtctgtcctg atgccatacc
     8581 agacagggtc gatcatcaca gtccagcagg ctgctgacca gtcgccggtc aaagcccggg
     8641 aaaaccccgg ccagccccgt gcggtgagca aaggtcagaa tatctatttc ctgccgcgta
     8701 ctgtgaatat ccgtgcgcag actgcactgc ccctggtagt aatccaggtc gagatacatc
     8761 agacgttccc gaaagtcacg acgcagcgtg cgctctgaca ccccgaattc agttgccagt
     8821 ttgcgaataa acagagtttc accggacacc aggcggctga taatcaacga caacctgacg
     8881 gcaagccggt catgtcgccg ctcctgaagc gtcatgaaag atttctcctg agaagttaac
     8941 tctatgaatt aataatgatt actttaaagg aaggagtgga cagggtatgg acaacatcgg
     9001 aactattttt tatgagcagt ccagccttgt gggcgctcgt ctgacagata atgatgcttc
     9061 gcctgattta attgatgata aaacgcagtg gaaaagacgt gtccacaatc aacacatcat
     9121 taacaaccag ttcatcattc gcagagaaaa ttgcgatatc agaagaatat ccagtcccag
     9181 acggctcgtg cgaccgacac caccgcatca cgcacggtac gaatcacctt tttcagtggc
     9241 gcaggcagta atgacgatga ctctgccgta tcgaaaacac ggcccacagc gtcaccgaaa
     9301 ctgtcacggg cctgcgactt taccggctct gtgcagagtc tcccgtgcag ttgtgtcacc
     9361 accgggctgg tggcgcggtc aggcagacag cgcatcatgg ctgccaccat atcctcaatg
     9421 ccccatccag tgctggcgga caccgcacag accggatgat gtggtttaaa catcgccata
     9481 atcgcagcct gcttcgcttt aagatttccc tgttggtgtg ttgaaggttt gctggtgagg
     9541 gtatcccatt catagcaggg ttcgattttg tcggcctggt taatcacaaa cagtaccttt
     9601 tgcctgtaag gttgcatcac accgagccag aactgctcgt ccaaggtcag tgcccgatca
     9661 tcggccttaa tgacccacag caccaggtcc agctccggca gcattcgacg gtacagggcc
     9721 cggtactcat gatcccgtcg cccgttttca cctacacccg gtaaatcgac aatcaccaga
     9781 ctgtgacgac cgctgcgcaa ccggaagcgc agtacgtcgc gagtacaggc gttcacgtca
     9841 ctcactgggg acacctcgcc ctggaaaagt tcgttgcaga gagaggattt acctgtacca
     9901 cttttgccca tgattccaat caccggctcg taatgggtca ggttctggat acgttcaaga
     9961 ataagctggc gaagtgtgca cggaagcgac gcaagcggtt gttcaaaggc ctgaaggccg
    10021 tcaggtttat tcatacagta gtctcagcgt gaaaaattaa aaaaccccgc gatccagtca
    10081 ggaaagcggg ggttcacgta gataatatct gtctgaaatt atttggaacg tactttacta
    10141 ttcttaatat tatataacct aaggatagca ctctctataa ttgcgttatt tgacatcgag
    10201 tctttcatag acatatctct cagcatatca tgagcttgct gagagatatt tacactcatt
    10261 gtcacaagtt ttttctcttc tttattttta ttcctatttt tttgagtaga ccaggcgctc
    10321 ttaaactttc taatgaacaa ctcgacagaa tctggagaat gcaggttgtt ttttctccaa
    10381 ataaagtaac ttgcatagca ccacgacatt atttcctcgg aagaattacc acaaatacgt
    10441 ttcagaccta ttgatgtatt cccccttaag taatctctta gccaagaaca gacatcgacc
    10501 ttactggatc taacacttct gaataaatca tgttgatcaa gtaactcagc ccactcaata
    10561 cttttatcct taatgaattc agccggtgtt gtaaaataca taggccattc atccatacaa
    10621 tgacgtatcg cttcaattct cacattatga tcaggttgca atgcttctgg agatagaaaa
    10681 tcaatattgt acttaggagt ttcttccttt aatttctcat ataactcaca agacaaccat
    10741 aagcttgctc ttggatcatt acgataccaa tcaagttcac tctcggatgg aaaacctctt
    10801 gtatagctat tatccacctc agcaaagaaa gaattaatgt tctgctcaga aacattctgt
    10861 gaaacaaggt aattgaaaac ccttaactga tattcccggt aatctttatc aaccccacca
    10921 gaatagaagt gataaccatt ctcaattaat ttattttcaa gatatttaat cttactactg
    10981 tcattcccca ctagcttaac cataccggca ccttccttga tatcactaag tgatttacag
    11041 tgaattaatg acttttagca taactccata ctgtatatca ataacattca
//
LOCUS       DAZFWP020000157        11007 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFWP020000157 DAZFWP020000000
VERSION     DAZFWP020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.157"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            171..1385
                     /gene="manD"
                     /locus_tag="ACHK0Z_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             171..1385
                     /gene="manD"
                     /locus_tag="ACHK0Z_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HGU4503976.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1452..2735
                     /locus_tag="ACHK0Z_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             1452..2735
                     /locus_tag="ACHK0Z_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4503977.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAASPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2987..3904
                     /gene="oxyR"
                     /locus_tag="ACHK0Z_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             2987..3904
                     /gene="oxyR"
                     /locus_tag="ACHK0Z_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU4503978.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3887..5287)
                     /gene="sthA"
                     /locus_tag="ACHK0Z_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             complement(3887..5287)
                     /gene="sthA"
                     /locus_tag="ACHK0Z_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU4503979.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5618..6268
                     /gene="fabR"
                     /locus_tag="ACHK0Z_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             5618..6268
                     /gene="fabR"
                     /locus_tag="ACHK0Z_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU4503980.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6268..6627
                     /gene="yijD"
                     /locus_tag="ACHK0Z_RS20245"
                     /old_locus_tag="pgaptmp_004049"
     CDS             6268..6627
                     /gene="yijD"
                     /locus_tag="ACHK0Z_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU4503981.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6667..7767)
                     /gene="trmA"
                     /locus_tag="ACHK0Z_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             complement(6667..7767)
                     /gene="trmA"
                     /locus_tag="ACHK0Z_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU4503982.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7881..8071
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8136..9980
                     /gene="btuB"
                     /locus_tag="ACHK0Z_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             8136..9980
                     /gene="btuB"
                     /locus_tag="ACHK0Z_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4503983.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9925..10782
                     /gene="murI"
                     /locus_tag="ACHK0Z_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             9925..10782
                     /gene="murI"
                     /locus_tag="ACHK0Z_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU4503984.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2541 a         2609 c         2944 g         2913 t
ORIGIN      
        1 taacgataat ggttaattat tcaggtggtt tttgagggta ttttttctcc tcggtggaaa
       61 tttttgtttt taatgtgatc acggttgaat aatctctttt tctctcttgt atggtagtag
      121 gcgtaaaatt aagtttgaaa cgtctattta caacacagga gattgcaata atgaaaatta
      181 ttgctgcaga tgtttttgtt acttgcccag ggcgtaattt cgttacgctt aaaataacca
      241 ctgaaagcgg actttgtgga ttaggagatg ccacgttaaa tggtcgcgaa ttgtcggtcg
      301 cttcttatct gaaagatcat ctttgtccac aactaattgg ccgcgatgcc agccgtatcg
      361 aagatatctg gcaattcttt tataaaggtg cttactggcg acgtggcccc gtcacgatgt
      421 cagccatttc tgccattgat atggcgttgt gggatatcaa agctaaagcg gcaaatatgc
      481 cattgtatca actgcttggt ggtgcttctc gcgaaggcgt gatggtttat tgccacacca
      541 ccgggcggac gattgatgaa gtccttgaag actatgcaaa gcaccagcag atgggcttta
      601 aggcgattcg tgtgcagtgc ggtgtaccgg gtatgcaaac tacttatggc ctggcgaagg
      661 gcaaaggcct ggcgtatgaa ccggcaacca aaggcctgtg gccagaagag cagttgtggt
      721 cttcagaaaa gtatctcgat tttacgccga agttatttga agcggtgcgt aataagttcg
      781 gttttaacga acatctgttg catgacatgc accatcgcct gacccccatc gaagcggcgc
      841 gctttgggaa gagcattgag gattaccgcc tgttctggat ggaagatccg acgcctgcag
      901 agaaccagga atgttttcgt ctgattcgcc agcataccgt gacgccgatt gcggtcggtg
      961 aagtgtttaa cagtatctgg gactgcaagc agttaatcga agagcagctg attgactata
     1021 ttcgcacgac gatcacccat gcaggcggca ttaccgggat gcggcggatt gccgattttg
     1081 catcgcttta tcaggtacgt accggttcgc atggcccttc agatttatcg cctgtttgtc
     1141 atgcggcggc gctgcatttt gacctatggg taccaaattt tggcgttcag gagtacatgg
     1201 gctattccga acaaatgctt gaagttttcc cgcacagctg gcgcttcgag gaaggttata
     1261 tgcacccagg cgatgaaccg gggctgggta tttctttcga tgagaaactg gcggcgaaat
     1321 atccgtatga cccggcgtat ctccccgtcg cacgactgga agatggcaca ttgtggaact
     1381 ggtaataacg gttcgcttaa ttattttctg aatagtcatc aaatcgatta attccgctca
     1441 aggagctaaa aatggacgtt gatgtgtcaa ccagtgttgc gggtaataaa ccgcaacgga
     1501 ttcgtcgtat tcagacagtg accctggttt tattatttat ggcggggatc gttaattttc
     1561 tcgaccgttc gtcattgagc gtggcaggtg aagcgattcg tggcgagtta ggattatcgg
     1621 cgacagagtt tggtgttttg ctttctgcat tttctctgtc ttatggtttt tcacaactac
     1681 cttccggtat tttgctagac cgctttggcc cacgaattgt tttaggcgca ggcttaatat
     1741 tctggtcatt aatgcaggca ttaacaggaa tggttaattc ttttagccac tttattttaa
     1801 tgcgtatcgg tctgggtatt ggcgaagcgc catttatgcc tgcgggagta aagtcgatca
     1861 ccgactggta tgcacaaaaa gaacgcggca cggcgctggg gatttttaac tcatctaccg
     1921 ttatcggtca ggctatcgcg cctcctgctc ttgtattgat gcagctggca tggggctggc
     1981 ggacgatgtt cgttatcatc ggcgtggcag ggattctggt tgggatctgt tggtacgcgt
     2041 ggtatcgcaa ccgggcgcag tttgtcctga ctgacgaaga acggacgtat ctctccgcgc
     2101 cggttaaacc gcgtccacag ctgcaattta gcgagtggct ggcgctgttt aagcatcgga
     2161 caacctgggg gatgatttta ggtttctctg gcgtcaatta taccggttgg ctctacatcg
     2221 cgtggttacc cggttatttg caagccgagc aaggtttcag tctggcgaaa accggttggg
     2281 tggcggcgag tcctttcctc gcggcggcag tcgggatgtg ggttaacggt attgttgtcg
     2341 atcgactggc gaaaaaaggc tacgacctgg cgaagacgcg taaaacggct attgtctgcg
     2401 gtttgatgat gtcggcatta ggcacgttgc tggtcgtgca atcttcctcg ccagcccagg
     2461 cggtagcgtt tatctcaatg gcgctattct gcgtgcattt cgctggaacg tctgcatggg
     2521 ggctggtgca ggtgatggtg tcagaaacaa aagtggcttc catcgccggg attcaaaact
     2581 ttggcagttt tgtttttgct tcctttgctc cgatcgtaac cggttgggta gtggatacca
     2641 cacactcgtt taatctggcg ttggttatcg cggcctgtgt gacgttcacc ggggcgctgt
     2701 gttacttctt tattgtcaaa gatcgcattg agtaatcgtt actatcaggc tctcttgctg
     2761 tgggcctgta gaataaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt
     2821 agttattcga gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc
     2881 cgctccgttt ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc
     2941 tattctacct atcgccatga actatcgtgg cgatggagga tggataatga atattcgtga
     3001 tcttgagtac ctggtggcat tggccgagca ccgccatttt cggcgtgcgg cagattcctg
     3061 ccacgttagc cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt
     3121 gatgttgctg gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt
     3181 agatcaggcg cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca
     3241 gggcgagacg atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct
     3301 gctaccgcat attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca
     3361 tgaagcacag acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat
     3421 cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat
     3481 gttgctggct atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga
     3541 tctggcaggg gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat
     3601 gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga
     3661 gacgctgcgc aacatggtgg cggcaggtag cgggatcact ttactgccag cgctggctgt
     3721 gccgccggag cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg
     3781 ccgcactatt ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct
     3841 ggcagaggcc atccgagcaa gaatggatgg ccatttcgat aaagtgttaa aacaggcggt
     3901 ttaaaccgtt taacgcagct acccgatagg cttccgccat ggtcgggtag ttaaaggtgg
     3961 tgttgacgaa gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga
     4021 tatgaataat ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt
     4081 cccgatggaa caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca
     4141 gatgtttaaa ctgggcgcgg cccacttcat atggcacttt catcgcggtc agctgctgtt
     4201 cggttttgcc cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa
     4261 tcagatgcgc ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat
     4321 aggccgctga tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct
     4381 gtgcggtctg atacatgctg ttgaccttca gctgtccgcg actgtcagtt tccagcccaa
     4441 tgttctgtaa cgccagcgaa tcggtattac cggtacgacc gttggcatag agcaggcagt
     4501 cagctttcag ttttttaccc gacttcagat gcatgatcac accgtcgtca cagccttcga
     4561 tcttctcgta ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga
     4621 gagaatctga catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat
     4681 ccacttttac atccataccg cggaagatcg acgcatattc acagccgatc actccagcac
     4741 catagataag tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa
     4801 tgcgtggatg ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa
     4861 caaatttttc agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat
     4921 gctcgtcaac aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc
     4981 cctgacgcat gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa
     5041 aagaagagcg gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa
     5101 tgcggctgac ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc
     5161 cgccaacatt ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca
     5221 ttgcagcgcc ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat
     5281 gtggcatggt agggcttacc tgttcttata cataaaagca acagaatggt aacattttat
     5341 cgcgggtaag ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt
     5401 tgtctgtttc ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt
     5461 ttaagcggca ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt
     5521 tttattgcgt taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta
     5581 tagtgccagc agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca
     5641 aaaagaaaaa acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg
     5701 cagcttcgcc agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc
     5761 tttttatcgg catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg
     5821 tttaatgcta cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt
     5881 gatccgcacc tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg
     5941 gttattattg cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga
     6001 aattcagcac ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg
     6061 tgcgtttact gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga
     6121 ggcgttggac gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact
     6181 gcgaatgatt tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat
     6241 tattccggga aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg
     6301 ctgctggcgt tggttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc
     6361 attgtgccat tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat
     6421 caacgttatc ttaatcgcac catgccggta ggcttgccgg gtctggcagc tgcctgtttt
     6481 attctcggcg tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct
     6541 aacttcttcc cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt
     6601 aaccgtaagc aggaagttgc tgagtaatcg gtattatgcc ggatgctggc gcatccggca
     6661 tgggttttac ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg
     6721 atcaaacaga gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca
     6781 taacgtttcc ggattgcagg agatatacag aatacgtgga tacgcctgca ccattttctc
     6841 ggtttcactg tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact
     6901 cttcaagtct atcccttgca ggcgattaaa ctcgcgtaca ccattcatcg cctgagtaaa
     6961 ttcttctgcc gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta
     7021 ttgcgcagca gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg
     7081 cgccagtgct aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga
     7141 gcctttggtt acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg
     7201 ggtaaaactg ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgttc
     7261 atcgatgtaa tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac
     7321 attcagattc tgcgcgcgca gtgcatcgcg cagggcttcc gcttcctgac gccactcatc
     7381 atccagcttt ttatggtaca acagggaaac caccgcctga ttactcagcg tggtgaggta
     7441 atcaatctgg aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc
     7501 cgtcatcaac tggttgatga gttcactggc agcggggaag ctatccacgc ggatgcggct
     7561 tttggtttgt tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa
     7621 ctccgcacgc atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga
     7681 aaacggtgcc atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg
     7741 ttctgttgga aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat
     7801 tgtagggatt gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct
     7861 ggacagtaac ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa
     7921 tccagtgcga atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg
     7981 cggacactgc catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc
     8041 ccgaagacct gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac
     8101 ctgcggcatc cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga
     8161 cggcgtgttc tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg
     8221 tcgttactgc taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg
     8281 tgacccgtca ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc
     8341 ttccgggcgt cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc
     8401 gcggtacaaa tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg
     8461 gggtgagtgg ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat
     8521 atatccgtgg gccgcgctcc gctgtttatg gttccgatgc aataggcggg gtggtgaata
     8581 tcatcacgac gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt
     8641 atcagaacta tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt
     8701 tgggcgatta tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc
     8761 aagcgcagac agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata
     8821 actttactga tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt
     8881 atgacgcgta ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa
     8941 gttgggacgc cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct
     9001 atagccatag caaagattac aactacgatc cccattttgg tcgttatgat tcgtcggcga
     9061 cgctcgatga gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg
     9121 gtagtattgg tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg
     9181 ttgagaatgg atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg
     9241 gcgattttac ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg
     9301 gaacctggca aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct
     9361 acgggacatc ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga
     9421 atctggaccc ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg
     9481 tgaactggcg tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc
     9541 acaccctgaa atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca
     9601 attttgatac cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg
     9661 cgattaccga cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact
     9721 ggcagttgta tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata
     9781 aggattactc atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc
     9841 ttgcggttgc gtatccggtc acctctcacc tgacagttcg tggtaaaata gccaacctgt
     9901 tcgacaaaga ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt
     9961 ctggcagcta caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt
    10021 gggttgtcgg tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct
    10081 ttcgataacg tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg
    10141 gtggcaattg tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc
    10201 aacaccgcca gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt
    10261 ggtgtcgtgc cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta
    10321 ctggcaaccc gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct
    10381 aatgaatgcc agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag
    10441 ctacatggcg aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga
    10501 atgaaagagc cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa
    10561 gaactcttac aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct
    10621 cgcagaacgg cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat
    10681 attgcctttt gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt
    10741 tacggcttcg aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc
    10801 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    10861 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaact
    10921 ccgacagaga aagcaaaaat aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac
    10981 cccgcgccgc tgagaaaaag cgaagcg
//
LOCUS       DAZFWP020000158        10939 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFWP020000158 DAZFWP020000000
VERSION     DAZFWP020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.158"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..502
                     /locus_tag="ACHK0Z_RS20265"
                     /old_locus_tag="pgaptmp_004053"
     CDS             <1..502
                     /locus_tag="ACHK0Z_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4503985.1"
                     /translation="KELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKG
                     LKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLD
                     VVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVL
                     AGSGLS"
     gene            771..3008
                     /gene="nrfB"
                     /locus_tag="ACHK0Z_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             771..3008
                     /gene="nrfB"
                     /locus_tag="ACHK0Z_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU4503986.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2995..5967
                     /gene="nfrA"
                     /locus_tag="ACHK0Z_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             2995..5967
                     /gene="nfrA"
                     /locus_tag="ACHK0Z_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU4503987.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5968..6858
                     /locus_tag="ACHK0Z_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             5968..6858
                     /locus_tag="ACHK0Z_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU4503988.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7041..7802
                     /gene="envY"
                     /locus_tag="ACHK0Z_RS20285"
                     /old_locus_tag="pgaptmp_004057"
     CDS             7041..7802
                     /gene="envY"
                     /locus_tag="ACHK0Z_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU4503989.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8315..9268
                     /gene="ompT"
                     /locus_tag="ACHK0Z_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             8315..9268
                     /gene="ompT"
                     /locus_tag="ACHK0Z_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU4503990.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9455..10939
                     /locus_tag="ACHK0Z_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             9455..10939
                     /locus_tag="ACHK0Z_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4503991.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHYLITPQKIENIYCPGNSEAEIQ"
BASE COUNT         2966 a         2697 c         2650 g         2626 t
ORIGIN      
        1 gaaagaatta aataatccag agcatgacag ttacgcaatc agtgaaaaga gtcacggcag
       61 agaagaaatc cgtcttcata ttgtttgcga tgtccctgat gaacttattg atttcacgtt
      121 tgaatggaaa ggtctgaaga aattatgcgt ggcagtctcc tttcggtcaa taatagcaga
      181 acaaaagaaa gagcccgaaa tgacggtcag atattatatc agttctgctg atttaaccgc
      241 agagaagttc gccacagcaa tccgaaacca ctggcacgtg gagaataagc tgcactggcg
      301 tctggacgtg gtaatgaatg aagacgactg caaaataaga agaggaaatg cagcagaatt
      361 attttcaggg atacggcaca tcgctattaa tattttgacg aatgataagg tattcaaggc
      421 agggttaaga cgtaagatgc gaaaagcagc catggacaga aactatctgg cgtcagtcct
      481 tgcggggagc gggctttcgt aatcttgccc tgcctgctca cctcctccag gcacttccac
      541 tacatgatga cctgatcggt gccaccactc ctcttcacta tgacgttaac ctcccctcct
      601 tgacaccacg cttttgtcta cattctacgc aaacggcaac ttttataacc agtgtaaaaa
      661 taacgtgccg caatagtcat acctattaat ggtaaaaagc tgtcacaatt cataaaaaac
      721 cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt gtggactggc
      781 ttcttgatgt ttttgcaacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg
      841 tcatcatgtt catcagcgga ctggacgatt tttttattga tgtcgtctac tgggtacgcc
      901 gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt
      961 ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca
     1021 tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg
     1081 ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct
     1141 ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa gccgactgtc
     1201 tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc gcttttgctg
     1261 gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt ctgttcaact
     1321 atctggtcga gcgtaaagat ctgattcaga tcccggtgta tccgttcgaa cgcgaatgga
     1381 cgcacttcac cagcatgact tacattgatg agttttcaga gctgcatggc aaagatgttc
     1441 cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc
     1501 gccgcgccgt gaccgctctg ttagctgacg gtgacggtat tgctttcgac gtgcagagtc
     1561 tgactgaaga ttacgacatt ggtttccgcc tgaaagagaa aggtatgacg gaaatttttg
     1621 tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt ttacagcacg
     1681 cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt tcgactgcag
     1741 ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa acccacaaat
     1801 ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta
     1861 actttgtcag cttcctcgcg atgctggtga tgctccagct tttgctgttg ctggcgtatg
     1921 aaagtttgtg gcccgatgcc tggcatttcc tttctatttt tagcggcagc gcatggttaa
     1981 tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag cgggtgattt
     2041 tcgtcactgg ctactacggt ctgacgcagg ggctgttatc tgtcctgcgt cttttctggg
     2101 gcaacctgat taacttcatg gccaactggc gcgcgctaaa acaggtactt caacacggcg
     2161 atccacgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtg actggcgata
     2221 cccgctcgtt gcgcccgtta ggccaaattc tgctggaaaa tcaggtcatc actgaagaac
     2281 aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cttgggcggt tcaatgctga
     2341 tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg
     2401 cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa atgccggcct
     2461 ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg
     2521 gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca
     2581 aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac ggggttacgt cactggtatg
     2641 cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc
     2701 tcacggaaca gcaggccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg
     2761 ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc
     2821 gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc gttatcagcc
     2881 aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac
     2941 tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc gaaaatgaag
     3001 gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc tttattgagt
     3061 accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat
     3121 cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac
     3181 gaagcaaccg ccatccgcga atttgaatat atacaccagc aggtgccgga taatattccg
     3241 ctgactttat accttgcgga agcctatcgc cattttggtc atgatgaccg ggcgcggctg
     3301 ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg cagtctggcg
     3361 gctattccgg ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa
     3421 gcgtgcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa tgccctgcgg
     3481 ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgcca
     3541 gaaggaaaaa ctctgcgaac cgatctgctg caacgggcaa tctacctgaa acaatggtcc
     3601 caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc
     3661 cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct ggcactgcaa
     3721 tcacagggga tcttcaccga tcctcagtca tatattactt acgcgaccgc gctggcttat
     3781 cgtggcgaaa aagcacgcct ccagcattat ctcaatgaaa ataagccgct gtttaccacg
     3841 gacgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag
     3901 gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg
     3961 ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc
     4021 cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg taacaaggct
     4081 gaatctctgc gtctggcacg attgctgtat cagcaagaac cggcaaatct tacccgcctg
     4141 gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc cgatttattg
     4201 ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg
     4261 gcgtctctgc tggaaagtca tccttacctg gcaacgccgg cgaaggtggc gattttatcg
     4321 aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgcagat
     4381 aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga tgccgccgcc
     4441 tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgct gtatgcatgg
     4501 cttcaggccg aacaacgaca accgaacgcc tggcaacatc gtgcggtagc ctatcaggcg
     4561 tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag tcttcacgac
     4621 atgagcaatg aggatctgct tgctgctgcc aataccgccc aggcggcagg aaatggtgca
     4681 gctcgcgatc gctggctaca acaggcagaa caacgtggac tgggaaacaa tgccctctac
     4741 tggtggctgc atgcgcaacg ttacattcct ggtcagccgg aactcgcact gagcgatctc
     4801 acgcgctcaa tcaatattgc cccttctgcc aacgcttacg ttgcgcgggc gacaatttat
     4861 cgccaacgtc ataatgtccc ggcggcggtg agtgatttgc gcgccgcgct ggaactggaa
     4921 ccgaataata gcaacatcca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc
     4981 gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga tccggcactg
     5041 atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac gcagcactac
     5101 gcccggctgg tgattgatga cattgataat caggcgctga taaccccact gaccccagag
     5161 caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg aggtcggtcg ccgctggacg
     5221 ttcagtttcg attcttctat cggcttgcgt tccggggcaa tgagcaccgc taacaataat
     5281 gtcggcggcg cagcgccagg gaaaagctat cgtagctacg ggcaactgga agccgagtac
     5341 cgcatcggac gcaatatgct gctggaaggc gacctgctct cagtttatag ccgcgtcttt
     5401 gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc cggcaccggt
     5461 ctgcgctgga agccgctgcg cgatcagatc tttttcctcg ccgtcgaaca gcagttgccg
     5521 ctgaacggcc aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc attctttaat
     5581 ggcggcaaat acagcgacga atggcacccg aacggttcag gctggtttgc ccaaaacctg
     5641 tacctcgatg cggcgcaata tatccgccag gatattcagg cgtggacggc agattatcgc
     5701 gtcagctggc atcagaaggt agctaacgga cagactattg agccttacgc tcacgttcag
     5761 gacaacggct atcgtgataa aggcactcag ggcgcgcagc ttggcggagt cggggtccgc
     5821 tggaatatct ggaccggcga gacgcactac gacgcctggc cgcacaaagt cagtctcggc
     5881 gtcgagtatc aacatacctt taaggcgatt aatcaacgta acggagagcg caacaacgcg
     5941 tttctcacca ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt gctgacactg
     6001 cttttggtca gccctttttc ctttgcgatg aaaggtatta tctggcaacc acaaaaccga
     6061 gatagtcagg ttaccgatac ccagtggcag gggctgatga gtcagttacg tttgcaaggc
     6121 ttcgataccc tggttttgca atggacccgt tacggcgatg cattcaccca gccagaacag
     6181 cgcgcgttat tgtttaagcg tgccgcagct gcgcaacagg ctggcctgaa gctgattgtc
     6241 gggctgaacg ccgatccgga attttttatg caccagaaac agtcgtccgc agcgctggaa
     6301 agctatctta atcgcctgct ggctgccgat ctccagcaag ccagattatg gagcgccgcg
     6361 cctggcgtaa cgccggatgg ctggtacatc agcgcggaaa ttgacgacct gaactggcgc
     6421 agcgaagctg cccgtcagcc tttgctaaca tggttaaaca acgcgcagcg gctgattagc
     6481 gatgtttcag caaaaccggt ttatatcagt agttttttcg ccggaaacat gtcgcccgat
     6541 ggctatcgcc aactgctgga acaagttaaa gcaaccggcg ttaatgtctg ggtacaggat
     6601 ggcagcggcg tggataaact taccgctgaa cagcgtgaac gttatctaca ggccagcgcc
     6661 gattgccaaa gtcccgcccc tgccagcggc attgtttatg aactgtttgt cgccggcaaa
     6721 ggcaaaacct ttacagcgaa accgaaaccg gatgcagaga tcgcctcgct gttagcgaaa
     6781 cgttcctctt gcggtaaaga cactctctat ttctctctgc gctatttgcc cgtcgcgcac
     6841 ggcattctcg agtattaaat ctcctccagg taagtcgggt acgacctggc ttacctcttt
     6901 cgctctcgtc agaattccat caagattaat tgctaaaaag cgtgctaaag aaagaagatc
     6961 gttatcggcg aacgggccga tcactcatgc aggaagcata gatcctttga gcggagtttc
     7021 tgatatctga ggagtgcgaa atgcaattga gcagcagtga accttgcgtg gtgatcctga
     7081 ccgaaaaaga ggtagaggta agcgtcaata accatgctac gtttaccctt ccgaaaaact
     7141 acctggccgc cttcgcgtgc aacaataacg tcattgaact ctcaacgtta aatcacgtat
     7201 taatcaccca catcaaccgt aacatcatca acgattatct gttgttttta aataagaact
     7261 taacctgtgt aaagccctgg tcgcggctgg caaccccggt tattgcttgt catagccgta
     7321 caccggaagt gttccggcta gccgccaacc acagcaagca gcaacccagc aaaccctgcg
     7381 aagcggagtt gacgcgcgta ttgcttttta ccgtattgtc taactttctt gagcaatcgc
     7441 ggtttattgc cctactgatg tatatcttac gcagcagcgt ccgcgacagc gtctgccgca
     7501 ttattcaaag cgatattcag cattactgga atctgcgaat tgtcgccagt tcgctatgtt
     7561 taagccccag cctgctcaaa aagaaattaa aaaacgaaaa taccagctat agccagattg
     7621 tcacagagtg tcgtatgcgt tacgccgtac agatgttatt gatggataac aaaaatatca
     7681 ctcaggtggc gcaattatgt ggctatagca gcacgtcata ctttatctct gtttttaagg
     7741 cgttttacgg cctgacaccg ttgaattatc tcgccaaaca gcgacaaaaa gtgatgtggt
     7801 gaagggcaaa gcggaaacgg ataagacggg cataaatgaa gaagaaatgg cgcgccctgc
     7861 aggattcgaa cctgcggccc acgacttaga agttcctaga acgacatttt aagtcaacaa
     7921 attaccgcgc catctctgcg ctcacacgtc ccactacctc aaaacatgta aagccttgca
     7981 agccattgtg aggccttatg tgtctcagtt ttgtccctct tttttgtact aaaaaacata
     8041 gcaattgagg ataaacctca tgctattttc gcttatatgc ctttaaaggc atggcgctta
     8101 aatagataaa aacaccacaa aagcataaaa aaccacacag taaaaccgca acataaaaca
     8161 ataacagata attaaaccaa aaacagatag cgcattgtga taatcattca atactaaaca
     8221 aaatataaac agtggagcaa tatgtgattg actcattaag ttagatataa aaaatacata
     8281 ttcaatcatt aaaacgattg aatggagaac ttttatgcgg gcgaaacttc tgggaatagt
     8341 tctgacaacc cctattgcga tcagctcttt tgcttctacc gagactatat cgtttactcc
     8401 tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa aagagcgcgt
     8461 ttatctagct gaagaaggag gccgaaaagt cagtcaactc gactggaaat tcaataacgc
     8521 tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta ttggggctgc
     8581 gggctggact actctcggca gccgaggtgg caatatggtc gatcaggact ggatggattc
     8641 cagtaacccc ggaacctgga cagatgaaag taggcaccct gatacacaac tcaattatgc
     8701 caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt accgcctggg
     8761 actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg gttcctatat
     8821 ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg gagaaagagc
     8881 aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa gttatcgtta
     8941 tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag catctgataa
     9001 cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca aagaccaaaa
     9061 ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa aagtttatgt
     9121 tgaaggcgca tggaatcggg ttacgaataa aaaaggaaat acttcacttt atgatcacaa
     9181 tgataacact tcagactaca gcaaaaatgg tgcaggcata gaaaactata acttcatcac
     9241 tactgctggt cttaagtaca ccttttaacg aagttaacca gattttctcc ccggtggtat
     9301 tgtataaccc cggggaattt gtttacagtt acaagtaggc aatatccact aattttgtaa
     9361 gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa cacaagacct caatatattc
     9421 aaatcttatc tttattccga tattcagaga cacaatgcga aaaataatta ctcatttcaa
     9481 agttgtttta acgttacttc taccagtaac cgtatctgcc cagcagatac agtggcaatc
     9541 ctgtatggcc agtcaattca accactggtt tggtgaggaa aaaccgtctc ctgacttact
     9601 atgtggttat ttgtctgttc cattaaaata tacagacaca ggcggagatg cttcttatga
     9661 aaaaaaatca caagtcaaac tagcgttgac aaaattgccg gcaaaaagca agcataaagg
     9721 aagtatcctg ataataagtg gtggtcccgg gttaccaggc ataaatcctt atattaactt
     9781 tgactggcca gtcacaaatc ttcgtgagtc atgggatatt attggatttg atcctcgagg
     9841 cgtcggacag tccactccga caataaactg ccggcaatca gatacagaga ctcaggaaaa
     9901 cataaccgaa aagcaacaag tattaaataa aattaatgcc tgtatccata ataccggagc
     9961 cgaagtcatt cgccatatcg gctctaacga ggctgtatac gatattgatc gtattaggca
    10021 agccttgggg gataaacaac tgacagccgt ggcgtattcg tatggaactc aaattgcagc
    10081 cttatatgca gaacgttttc cctacaacgt aagatctatc gttcttgatg gagtcgtcga
    10141 tatcgatgac ctggaggaca acttcacatg gcaactcaaa caggcacaga gttatcagga
    10201 aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa agttgcccgc tttcttcaga
    10261 cagagataag gcaataactc agttccatga gctattatca aaattacatc acaaaccttt
    10321 attagacagt aagggagaaa atatatcttc agatgaactc atatcattaa caacagacct
    10381 tctgctatgg cgttcatcat ggccaaccct tgcaactgcc atacgccagt tctctcaggg
    10441 gattgtcagt aatgaaattg aaactgcgct cagtgctccg atagcctcag aagagtcaag
    10501 cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat gagcaattaa caccagaaga
    10561 gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg gctattaact ttgacaatgg
    10621 acgttccgat tcacctgatt tttgtgaatt atggccaata catagcgacc tgaacaaaac
    10681 tcgcctgaaa aatactgttc tgccctctgg tttactgttt gtagcacaca aatacgaccc
    10741 aacaacgccc tggattaatg cccgtaagat ggcagagaaa ttttccagcc cgttactaac
    10801 aataaatggt gatgggcata cattagctct caccggagtt aatttatgtg tagataaagc
    10861 agttgtacat tacctgatca ctccacaaaa aatagaaaat atatactgcc caggaaattc
    10921 tgaagcagaa atacaataa
//
LOCUS       DAZFWP020000159        10808 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFWP020000159 DAZFWP020000000
VERSION     DAZFWP020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.159"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..526
                     /gene="hpt"
                     /locus_tag="ACHK0Z_RS20300"
                     /old_locus_tag="pgaptmp_004060"
     CDS             <1..526
                     /gene="hpt"
                     /locus_tag="ACHK0Z_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU4503992.1"
                     /translation="VEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRGSFMF
                     MADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDIIDSGN
                     TLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDYAQRY
                     RHLPYIGKVILLDE"
     gene            complement(567..1229)
                     /gene="can"
                     /locus_tag="ACHK0Z_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             complement(567..1229)
                     /gene="can"
                     /locus_tag="ACHK0Z_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU4503993.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1338..2264
                     /locus_tag="ACHK0Z_RS20310"
                     /old_locus_tag="pgaptmp_004062"
     CDS             1338..2264
                     /locus_tag="ACHK0Z_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4503994.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2261..3031
                     /locus_tag="ACHK0Z_RS20315"
                     /old_locus_tag="pgaptmp_004063"
     CDS             2261..3031
                     /locus_tag="ACHK0Z_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4503995.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3136..3576
                     /locus_tag="ACHK0Z_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             3136..3576
                     /locus_tag="ACHK0Z_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4503996.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3640..4869
                     /locus_tag="ACHK0Z_RS20325"
                     /old_locus_tag="pgaptmp_004065"
     CDS             3640..4869
                     /locus_tag="ACHK0Z_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4503997.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(4873..5253)
                     /gene="panD"
                     /locus_tag="ACHK0Z_RS20330"
                     /old_locus_tag="pgaptmp_004066"
     CDS             complement(4873..5253)
                     /gene="panD"
                     /locus_tag="ACHK0Z_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU4503998.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5527..6429
                     /gene="rpnC"
                     /locus_tag="ACHK0Z_RS20335"
                     /old_locus_tag="pgaptmp_004067"
     CDS             5527..6429
                     /gene="rpnC"
                     /locus_tag="ACHK0Z_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU4503999.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            complement(6503..7354)
                     /gene="panC"
                     /locus_tag="ACHK0Z_RS20340"
                     /old_locus_tag="pgaptmp_004068"
     CDS             complement(6503..7354)
                     /gene="panC"
                     /locus_tag="ACHK0Z_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU4504000.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(7366..8160)
                     /gene="panB"
                     /locus_tag="ACHK0Z_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             complement(7366..8160)
                     /gene="panB"
                     /locus_tag="ACHK0Z_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU4504001.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8273..9616)
                     /locus_tag="ACHK0Z_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             complement(8273..9616)
                     /locus_tag="ACHK0Z_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HGU4504002.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            complement(9600..10196)
                     /locus_tag="ACHK0Z_RS20355"
                     /old_locus_tag="pgaptmp_004071"
     CDS             complement(9600..10196)
                     /locus_tag="ACHK0Z_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4504003.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(10223..>10808)
                     /gene="yadL"
                     /locus_tag="ACHK0Z_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             complement(10223..>10808)
                     /gene="yadL"
                     /locus_tag="ACHK0Z_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HGU4504004.1"
                     /translation="RYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGTCTATLV
                     NDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRATCEGTA
                     NDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNAANAVVY
                     YPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
BASE COUNT         2695 a         2512 c         2711 g         2890 t
ORIGIN      
        1 tgtagaagta atgatccccg aagcggagat taaagcgcgt atcgccgaac tgggtcgtca
       61 gattactgag cgttacaaag acagcggcag cgatatggtg ctggtgggtc tgctgcgtgg
      121 ctcatttatg tttatggcgg acctgtgccg tgaagttcag gtatctcatg aagtcgactt
      181 tatgaccgcc tccagctacg gtagcggcat gtccaccacc cgtgatgtga aaatcctcaa
      241 agatctggat gaagatatcc gtggcaagga cgtgctgatt gttgaagata tcatcgactc
      301 ggggaataca ctgtcgaaag tgcgtgagat cttaagcctg cgcgaaccga agtcgctggc
      361 gatttgtacg ctgctggata aaccgtcccg tcgtgaagtg aacgtcccgg tagaatttat
      421 cggtttctcg atcccggatg agtttgtggt gggttacggc attgattacg cacagcgtta
      481 ccgtcatctg ccgtatatcg gcaaagtgat tctgctggac gagtaagtgt gaagttgccg
      541 gatgtgttgc atccggcatg gcatttttat ttgtggttgg cgtgtttcag cttgaggttg
      601 gaaatcccgt gacggtaacg ttgctcaagg gtttcgcggt tggtggcggt aacatccaga
      661 tcacgcagca agccgtcgtg aatgccgtag gcccagccgt gaatggtaac tttctgcccg
      721 cgtttccacg ctgattgcat aatggtggag tggcccaggt tatacacctg ttccatgacg
      781 ttcagttcac acaaggtatc cagacggcgc tcttgcggca tttcgccgag caatgagcta
      841 tgtttgaacc agatatcgcg gatatgcagc agccagttgt tgataagccc cagttccggg
      901 ttttcaacgg cggcttgtac gccgccgcaa ccgtagtggc cacagataat aatgtgttca
      961 acttcgagta catccactgc atactgaacc acggaaaggc agttcaggtc ggtgtgaatg
     1021 accaggttag caacattacg gtgaacaaag agttcgcccg gctcaagacc ggttaaacgt
     1081 tctgcaggaa cgcgactgtc ggaacatcca atccatagaa agcgcggttt ttgcgcttgt
     1141 gccagtttct caaaaaaccc gggatcctct tccaccagca tttttgacca tagtgcattg
     1201 ttgctgatga gtgtatctat gtctttcatg gaggttaacg acctgtaacc aaataattac
     1261 gtttggctaa tatagggcaa ctccgggacg atttaaacca cagataaagt gtaagaacgt
     1321 aaggtaagta aaaatttatg accattgcac tggaacttca acagcttaaa aaaacttatc
     1381 caggcggcgt tcaggcgctt cgtggaatag atttgcaggt cgaagcgggt gatttttatg
     1441 cgcttctcgg gccgaacggg gccgggaaat cgaccactat cggtattatc agctctctgg
     1501 taaataaaac ctccgggcgg gtcagcgtat ttggttacga tctcgagaaa gatgtcgtga
     1561 acgctaaacg tcagttggga ctggtgccgc aggaatttaa cttcaacccg tttgaaaccg
     1621 tgcagcaaat tgtggtaaat caggcagggt actacggtgt ggagcgcaaa gaggcgtaca
     1681 tccgcagcga aaaatatctt aaacaactcg atctatgggg aaaacgcaac gaacgtgcgc
     1741 gtatgttatc tggcgggatg aagcgccgtt taatgattgc ccgtgcgtta atgcatgaac
     1801 ctaaactact gattctcgac gaaccgaccg caggcgtgga tattgaactt cgccgctcaa
     1861 tgtggggctt tttgaaggat ttaaacgaca aaggcaccac catcattctc accacacact
     1921 acctggaaga agcagaaatg ctgtgccgca atatcggcat tattcaacac ggtgagctgg
     1981 tggaaaatac ctcgatgaag gcgctgctgg ctaagctgaa atcggaaacc tttattctcg
     2041 atctcgcacc gaaaagtccg ttaccgaagc tcgatggcta tcagtatcga ctggtcgata
     2101 ccgcgacgct ggaagttgaa gtgctgcgtg agcaggggat caacagcgta tttacgcagt
     2161 taagtgagca gggcattcag gtattaagta tgcgtaacaa agctaaccgt ctggaagagc
     2221 tgtttgtttc actggttaat gaaaaacaag gagatcgcgc atgatgcatc tttactgggt
     2281 ggcgctaaaa agcatctggg cgaaagagat ccatcgcttt atgcgtatct gggtgcagac
     2341 gctggtgccg ccagtcatca ccatgaccct ttactttatt atcttcggta acctgatagg
     2401 ttcgcgtatt ggcgatatgc atggcttcag ctatatgcag ttcatcgtac cggggctgat
     2461 catgatgtcg gtgatcacca atgcctacgc taacgtcgcg tcatcatttt ttggtgccaa
     2521 gttccagcgt aatattgaag agctgctggt agcgccggtt ccgactcacg tcattattgc
     2581 cggatatgtc ggcggtggcg tggcgcgtgg tctgtttgtt ggcattctgg tgacggcaat
     2641 ttcactgttt tttgtgccgt ttcaggtgca ttcgtgggta ttcgttgcct taaccctggt
     2701 gctcacggcg gtgctattct cccttgcggg tttgctgaac ggcgtgtttg ccaaaacgtt
     2761 cgatgacatc agcctggtgc caacctttgt gttaacgcca ctcacgtatt tgggtggggt
     2821 cttttactca ctgactttgt tgccgccgtt ctggcaaggg ctgtcgcacc tgaacccaat
     2881 cgtttatatg atcagtggtt tccgctacgg cttcctcggt atcaatgatg ttccgctggt
     2941 cactaccttt ggcgtactgg tggtctttat tgtggcgttt tatttgatct gttggtcgct
     3001 gatccaacgt ggacgtggtt tgcgtagcta aggctatttc ctctcctctg gatttggggg
     3061 agaggagttt tgacggctat caccctttat caacaatggt cagggtagac tgattttcgg
     3121 ctaaggagga aggcgatgtt aggttgggta attacctgtc acgatgaccg ggcgcaagag
     3181 atactggatg cgctggagaa aaaacatggg gcgcttcttc agtgccgggc cgtgaatttc
     3241 tggcgtgggt taagctctaa tatgctcagc cgcatgatgt gcgatgctct gcatgaagcg
     3301 gactctggtg agggtgtcat cttcttaacc gatatagccg gagcgccacc gtaccgcgtg
     3361 gcttcattat taagccacaa acactcccgt tgcgaagtga tttctggtgt cacgttaccg
     3421 ttaattgaac agatgatggc ttgccgtgaa accatgacca gttcagagtt tcgcgagcgt
     3481 attgtcgaac tgggtgggcc ggaggtgagt agtctttggc accaacaaca aaaaaatccg
     3541 cctttcgtcc tcaaacataa tttgtatgag tattaaccca cgattcttat agcgcttttg
     3601 ctacaataaa agcgttgttt caccctcggt tattttttca tgtacaaaca agctgttatt
     3661 ctcctgctga tgctgtttac cgcaagtgtc agtgccgcgt tacctgcccg ttatatgcaa
     3721 accatcgaaa atgctgcgat ctgggcgcaa attggcgaca agatggtgac cgtggggaat
     3781 attcgggccg ggcaaatcat tgccgtggag cccactgccg caagttatta cgcatttaat
     3841 tttggctttg gtaaagggtt tatcgataaa gggcatctcg agccagttca ggggcgacaa
     3901 aaagttgaag acggtttggg tgacctcaac aagccgctga gtaatcagaa cttaattacc
     3961 tggaaagata cgccggtcta taacgcgccg agtgcgggca gtgcgccatt tggggtactg
     4021 gcggacaatt tgcgctaccc gattttgcat aaactgaaag acaggttaaa tcaaacctgg
     4081 tatcagatcc gtattggcga tcgactcgcc tatatcagcg cgctggatgc ccaacccgat
     4141 aatggcctgc cggtgctaac ctatcaccat attctgcgcg atgaagaaaa cacccgtttt
     4201 cgccatactt cgacgaccac ctcggtacgc gctttcaata accagatggc ctggctgcgc
     4261 gacaggggat acgcgacact gagcatggcg cagctggaag gttacgtgaa gaataagatc
     4321 aatctccctg cgcgagcggt ggtgattacc tttgatgatg gcctcaagtc ggtgagccgc
     4381 tatgcgtatc ctgtattgaa acaatatggc atgaaggcga cggcgtttgt tgttacctcg
     4441 cgcatcaaac gtcacccgca gaagtggaac ccaaaatcgc tgcaatttat gagcgtttct
     4501 gagcttaacg aaattcgcga tgtttttgat tttcagtcac atacccattt tttgcatcgg
     4561 gtggatggct atcgccgacc catattactg agccgtagtg agcacaatat tctgtttgat
     4621 tttgcacgtt cacgccgcgc tctggcgcaa tttaatccgc atgtctggta tctttcgtat
     4681 ccgtttggcg gatttaatga caaagccgtg aaggcagcaa acgatgccgg atttcacctg
     4741 gcggtgacaa ccatgaaagg caaagtaaaa ccgggggata atccgttgtt actaaaacga
     4801 ctttatatct taagaacgga ttcgctggag acgatgtcgc ggctggtgag taaccagccg
     4861 cagggataac aatcaagcaa cctgtaccgg aatcgctttc gcggtacgtt tcatttcatt
     4921 gtcgccttca aaataggcga cattgggtcg ccaggtgcga gcttcttcat ctggcatggt
     4981 aacgaagctg gcgatgatga caatatcgcc gacgctggcg cagtgggccg ccgcaccgtt
     5041 aacagaaata attctcgaac cgcgttctgc cgcgatggca taagtggaga aacgcttgcc
     5101 gttggtgaca ttccagatat cgatggcttc gttttcgaga ataccggctg cgtcaagaaa
     5161 atcctggtca atggcgcaag aaccttcata gtgcaggtcc gcatgagtca ctttcacgcg
     5221 gtggagtttg ccctgcagca tcgtgcgaat cataacttct acctttctac cctgtcgtta
     5281 acgaagcagg cggtgcctgc tttgaggaaa ttctcacgca gtattgcccg atttttgatg
     5341 agtgtctact catctgatgg catttgcgtc agcagtttgc gtaccgcgcc gaagcgtgcc
     5401 atgtatcgcc tgtaacgaat tgaaatttct ctgaacgctg ctcggtaaac actaaagagg
     5461 cggctgacga cggcgcgcct tgactggata ctgccttcac gcaagacaac acggaagaca
     5521 gggaagatgg atgcaccgag taccacaccg catgacgcgg tatttaaaca atttttaatg
     5581 catgcggaga cggctcgcga ctttctggag atacatttgc cagtggaatt acgagaactt
     5641 tgtgacctca acacgcttca tttagagtcg gggagtttca ttgaagagag cctgaaagga
     5701 cacagcacgg acgtgctcta ttccgtgcaa atgcagggca atcccggtta tctgcatgtt
     5761 gtgattgaac accaaagcaa gccggataag aaaatggcct ttcgcatgat gcgttattct
     5821 attgctgcca tgcaccggca tctggaggcg gatcacgata agctgccgct ggtggtgccg
     5881 atactgtttt atcagggcga ggccacacct tatccgctct caatgtgctg gtttgatata
     5941 ttttactcgc cggagctggc acgacgcgtc tataacagtc ctttcccgct ggtggatatc
     6001 accatcacac cggatgacga aatcatgcaa catcggcgga ttgcgattct cgaactactg
     6061 caaaaacata ttcgccagcg cgacttaatg ttattgctgg agcaactggt cacgctgatt
     6121 gacgaagggt acactagcgg aagtcagtta gttgccatgc aaaactatat gctgcaacgc
     6181 ggtcatactg aacaagcgga tttgttttat ggtgtgctga gagacaggga aacgggaggg
     6241 gagtctatga tgacgctggc gcagtggttt gaagagaaag ggattgagaa ggggattcag
     6301 cagggaagac aggaggaaag gcaagaattc gccctgcgtc ttctaagtaa aggaatgtct
     6361 cgggaagacg ttgcagagat gacaaattta tctcttgctg agattgataa ggtaattaac
     6421 cttatttaag ttacctgtgt tatgacagat gacgtggggt aaattaataa ctggcgccat
     6481 cagccgtagc gccagttaag tattacgcca gctcgaccat tttgttgtcg atcaggcgag
     6541 catcgccaag ccaggcggct accagaatta ctgcccgttt gctggtttca gaaacttcca
     6601 gcaatgtgtc ggcatcgcga atctgaatat catcggcgcg gaagcctttt tcattcagtt
     6661 cttgccccgc aatggtaata atttcatcga gatcccgttc cccagcctgc aatttgtcag
     6721 caatcgaact taaaactttg tacagaccag gcgcaatttt gcgttgttcc gccgtcagat
     6781 aaccgttacg ggaacttagc gccagaccgt ctttggcgcg cataattggc acaccgacaa
     6841 tctcaatatc gaagcccata tcggcaacca ttttgcggat cagcgccagt tgctgaaagt
     6901 ccttttcacc gaagcaggcg atgtccggct ggaccaggtt aaacagcttg ctgacgatag
     6961 tcgaaacgcc gcgaaaatgc cccggacggc tcgcgccttc cagcatggtc gaaagcccgg
     7021 gaacgtcaac gtaagtatgg gtttcagtac cgttcgggta gatctctttt accgaagggg
     7081 cgaaaactaa atccacttta cgtttgttta gcttctcgca gtcctcctgc aaggtccgtg
     7141 gataacgtgc cagatcttcc gggcggtcga actgcatcgg gttaacgaaa atactgacga
     7201 cgaccacatc ggcgcgggct ttggcttcgt cgaccagctt catatggcca tcgtgcagat
     7261 tacccatagt aggcaccagc gccacgcgct tgccttccat acgcaggcgg cgaatttgct
     7321 gacgcagcag cggcagggtt tcgataatta acacaacgtg actccttaat ggaaactgtg
     7381 ttcttcgccc ggataaacgc cggactccac ttcagccata tactgccgca cagccgcgcg
     7441 gatgtcgccc gtttcggcga ggaaattttt agcgaattta ggaatgtgac cgccggtaat
     7501 gccgaaggcg tcgtgcatca cgaggatctg cccgtcagtg acgttgcctg cgccaatgcc
     7561 aataaccggg atcgccagtg cttcggtaat acgttttgcc agttcaaccg gcacgcattc
     7621 cagcaccagc agctgtgccc cagcggcttc taaggctaat gcatcgctga gcagttgatc
     7681 gcccgcttca tcgccgcgcc cctgaacttt gtagccaccg aaaatattca ctgactgtgg
     7741 ggttaaacct aagtgaccac atacaggaac ggcacgttcg gtcagcattt ttacggtttc
     7801 gaccagccac tcaccgccct caattttgac catattggca ccggcacgca taaccgttgc
     7861 ggcgttttca aaggcttgtt ccggcgtggc atacgccata aacggcaggt cagccaacag
     7921 caggcagttt ggcgcgccgc gacgtacggc ggcggtatga taggcgatat cctcgacggt
     7981 aacgggaagg gtggagtcgt ggccctgaac cgtcatgccc agcgaatcgc ctaccagcat
     8041 gacgttaagt ccttcatcag caaatagttt cgcgaagctg tagtcgtaag cggtgatggt
     8101 cgcgaaacgc tttttatcct gcttacattt ctgaagtgaa gcaatggtgg tcggtttcat
     8161 aacgtgtcct gataaatgat gtcgtgttgc ctggcatttt atcagtcaca ttggtggggg
     8221 caatgattta tcaattaccg caccgccaga tgatggcggt gcggtggact tattaagggt
     8281 aggttacctg gaaagtactg gtggctttaa actcaccagc ttctattggt atagttccat
     8341 cctgttgtag cgtggcctgg aaatggagag ggtatgttat acctgtatct tgatccgggt
     8401 aaactcctcc tgttgtatcg tctttgcctc ttgggtcgta atctttataa acagaatttg
     8461 aatcattagg tttcaatgtc attagaggat ttttactagt tgctaaacct tctatgagta
     8521 caccgactcc ttttgctgcg tcattacctg tgagagtatt accaaggagt tgcccgtttt
     8581 cagtaccaac ctttgttgaa actaattttg tctcaatatt agtcacacga acgcagtttt
     8641 gaagtgaaat atcaaaagga accggtgtgg caccgtttct gatttgttct gcactatatt
     8701 cacccatttt taccgtagaa ccagtcacag aagttccact aagcatagcg gtaaaacaag
     8761 tgggattagt caacttgaca ttgagcagat cgaaatcgat tttgataaaa taactatgct
     8821 catttatacc ttggcctgcg ccataggctt taaatgaata cagataattt gatgagctgg
     8881 ataatttgat ctgttgagtt actgttggat caaagtttgt atctgtatag aattcaattg
     8941 taagggtctg ataaatacca cctacagccc agtatttacg ggtgctattc gcattattgc
     9001 aactccaata taacacgtct tcgcttggat tataccacga aaaatattgt tctgcagagt
     9061 caccaatgta tatcccaggt gaactaacgt tagttacggt accgtaagct gaccacatgt
     9121 ttgaaattaa taatgtgtaa tacaaaccag gaactgatgt attaaataat ttatgggagc
     9181 catacatttt acctgagtca accattgcat tttcaatacg cattgcgcta ccacttgtgc
     9241 tatcacttct gttgcaataa agaaaaccac caccagaagc ggtatctaac tgactccact
     9301 gtaattttac acctgtaggt ccaagctgcg tcggtttgta gaaaactaaa ttatttgtac
     9361 ttgttgcata tctgaatatg acagtagcat cgccattcgg ccccgtttct ctccattttt
     9421 gatctgacgg accaacgccg taactattcc caacaatata tcctacgtta tcatcagtgc
     9481 cagcactcgc ggtataacaa ctcaagcaca gaattaaaaa aagaaaatgt ctgaagaatt
     9541 tcttcattat cactccacca tctactgctg taacagtcgc agttatttgt tttgatctat
     9601 tattcgtagt acacatcaac taatatatga ctcattacct ggccagatat gacatccagc
     9661 gcgggatcga ttttttgcat tctggcataa aatttccatg aagaattatg cagggcagaa
     9721 gtcattactg gatacacgga acgagaattt cctgctgaac taataacatt gaatatattg
     9781 gtcgggtcct gaatggaaaa gagaacgaca ccgacatttt tagccccggt tgcgacttca
     9841 ttggcaaata tttgccttga acctgcagcc atgacaccgc taagggctct aaaatcaata
     9901 tgtaactgat taagcacatc gccagttccc tgtaatgcac agtctgatac tgtgacagtg
     9961 aactcttttc cacctggttg atagctttca ggagtaacat tatctgaaaa atatcccacc
    10021 ccaacgacgc caagatcaat attaccctgg ttactgattc cgcttttgca agtgctgttt
    10081 tttacattgg caacaaggtc aatatcctgc ccagcgatgg ctggataggt aatgggaaac
    10141 tgtagtgaca gaaacagaat tattctcttc atcagcttac gttgagtggg atgcatatat
    10201 agttcctgga tctgtcagtc tgttagttat aggttactgt aaatgtggca ggtgcagaaa
    10261 atttacccgc agtgacattg cttacggttt tatttttttc gacgaccagt cgagcactca
    10321 tcggataata aacaaccgcg ttggctgcat tggagatagt gacctcttga gatgctggtg
    10381 tcacacaact aaattgtgtt gcgctccctg ttgccggagt ttcggtgctc cagacttcaa
    10441 cggcgacggc acttgcttta tctgcggctg tggatccatt tgcgaacccc gcaccgtcat
    10501 tagcagtacc ttcgcatgtg gctcgcttgg ttaattttat ttgtgctttt ttattgggga
    10561 tacccgcaca gtctttgaat ttgagtttga atgttttaac tttggtcttg gcattgattt
    10621 cagaaatata tacatcgcca aagtcaacaa cagaaatgtc ctgatcagag tcatttacta
    10681 atgtagcagt acaagtacct atttctacgg tagtaatcac ggtcagccca atgctgtctg
    10741 ttgccgcata agagggtacg ctgaacaatg aggcagccaa aacgacactg ctggataatc
    10801 cataacgt
//
LOCUS       DAZFWP020000160        10787 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFWP020000160 DAZFWP020000000
VERSION     DAZFWP020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.160"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(102..1766)
                     /gene="glnS"
                     /locus_tag="ACHK0Z_RS20365"
                     /old_locus_tag="pgaptmp_004073"
     CDS             complement(102..1766)
                     /gene="glnS"
                     /locus_tag="ACHK0Z_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4504005.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1970..3916)
                     /gene="nagE"
                     /locus_tag="ACHK0Z_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             complement(1970..3916)
                     /gene="nagE"
                     /locus_tag="ACHK0Z_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU4504006.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDY
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4249..5049
                     /gene="nagB"
                     /locus_tag="ACHK0Z_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             4249..5049
                     /gene="nagB"
                     /locus_tag="ACHK0Z_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4504007.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKICSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5109..6257
                     /gene="nagA"
                     /locus_tag="ACHK0Z_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     CDS             5109..6257
                     /gene="nagA"
                     /locus_tag="ACHK0Z_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4504008.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6266..7486
                     /gene="nagC"
                     /locus_tag="ACHK0Z_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /gene_synonym="nagR"
     CDS             6266..7486
                     /gene="nagC"
                     /locus_tag="ACHK0Z_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU4504009.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7534..8286
                     /gene="nagD"
                     /locus_tag="ACHK0Z_RS20390"
                     /old_locus_tag="pgaptmp_004078"
     CDS             7534..8286
                     /gene="nagD"
                     /locus_tag="ACHK0Z_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU4504010.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8543..10207
                     /gene="asnB"
                     /locus_tag="ACHK0Z_RS20395"
                     /old_locus_tag="pgaptmp_004079"
     CDS             8543..10207
                     /gene="asnB"
                     /locus_tag="ACHK0Z_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU4504011.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10588..10664
                     /locus_tag="ACHK0Z_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     tRNA            10588..10664
                     /locus_tag="ACHK0Z_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10622..10624,aa:Met,seq:cat)
     gene            10673..10757
                     /locus_tag="ACHK0Z_RS20405"
                     /old_locus_tag="pgaptmp_004081"
     tRNA            10673..10757
                     /locus_tag="ACHK0Z_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10707..10709,aa:Leu,seq:tag)
BASE COUNT         2676 a         2791 c         2752 g         2568 t
ORIGIN      
        1 gaaaagcaga aaaaacgccg cagagtgcgg cgtttgggat tgtttgctat ttcttgccgg
       61 atgcgacgta aatgcctcac ccggcctggt agaacttaaa attactcgcc tactttcgcc
      121 caggtatcac gcagcccaac ggtgcggtta aataccggtt tttccgctgt ggaatggcgg
      181 ctgtcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc aaccgcatct
      241 ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac
      301 aggaaatcat ccgcagcacc agggttcggc acgctgaaca gacggtcgta cagacggatt
      361 tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac tttacgacca
      421 tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt ggtgatattg
      481 ccttcggcat ctttctccac gcgttcagcc ttaatgacgt aagcattacg cagacgcact
      541 tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggcgcga
      601 tcgatccaaa tctcaccgct aaacggcacc tgacgactcc ccatttccgg tttgttcgga
      661 tggttcggca tggtgaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc
      721 ggatcgataa ccgccattgc gcgcggcgca ttttcgttga gatcttcacg gatgcaggat
      781 tccagcgatg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac
      841 tcacgaatag aagcagcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc
      901 gggtcatccc agccttcaac gtgcttgtcg gtcaccagca ggttcagctt acgcttggac
      961 atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg aacaggaatg
     1021 gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt cctgaaactc aagcgtacac
     1081 agagagtgcg taataccttc cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg
     1141 tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatacg gtacagcacc
     1201 ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta
     1261 ccttcttcaa aaccaccggc acgcattttt tcgaacagcg ccaggttctc ttcaacgctg
     1321 cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg tgccgcggta ttcgcggatc
     1381 tgttccggcg tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag
     1441 gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct
     1501 aaccactcta cgtcgttttt gatcgactca acatactcga tatcttcttt taccgggtta
     1561 gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg
     1621 cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg
     1681 tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta
     1741 gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg
     1801 gcgtatgatc ttataagcgg gacaggctga caactgttag ttttttaaaa ttgataatct
     1861 aacaaggatt atggggatga ttgatgaaaa aaaaaagcgg cagagaagat ctccaccgct
     1921 tgtcattgtt ggatgcgacg ctcaagcgtc gcatcaggca taaagcagat tactttttga
     1981 tttcatacag cggtgtttga cccgccacaa catggccctg agctttaatg atcaagccac
     2041 tgaaatagtc gatattgctg caaaccaccg ggctaatcat cgagcgggcg ttagcgttca
     2101 ggtaatccag atccatttcc agaatcggtt gccctgcgct tacctgcgca ccctcttcca
     2161 ccagacgttt aaagccttta ccttccagcg ctacggtgtc gatacccata tggacgacga
     2221 tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt ggtgttgaag attttcacga
     2281 ttgtccctgc ggctggtgat acgacgattt tatctgtcgg tttcaccgcc acaccgtcac
     2341 ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc cagtgccacg acatcaccgg
     2401 taatcggcga taccagctcc gcgatagata ccgcgtttgg tacagcctgc ggttttgcta
     2461 caggcgcggc agttgccgga gttgcttcag ctgacgcagc ggctaccgga ccacgggcaa
     2521 cgactttctt catcgcatcg ccgatggatt ctgctttcgc gccaacaatc acctgaatag
     2581 tctgtttgtt cagtttcact acaccagaag cacccagacg tttacacatc gtatcgttga
     2641 cgcgggcaga gtcagccacg gtcaggcgca gacgggtgat acaggcgtca attgctttca
     2701 ggttgtcagt gccgccaacc gcagcaatat agttggtcgc cagttgattg aggccttctt
     2761 cagtgttgct gttggcttct tcagtaacga tctcgtcttc tttatcttca cgacccggcg
     2821 ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac gaagtagata gcgaagaaga
     2881 caacacccat aaccagcagc atccagacgt tctggctggc ggccggcagg ttatacatca
     2941 acgcgtagtc gatagccccc gcagagaaag agaagccagc atggataccc agcagtgttg
     3001 ccacaaacag gctgataccg gtcagcagtg cgtgcaggag gtacagcagc ggtgcaagga
     3061 acatgaacag gaattccagc ggctcagtca caccggtcag gaacgcagta acagcaacag
     3121 aaagcagcat cccgccaacc atcggacgac gctctttcgg tgctgcgaag tacatcgcca
     3181 gcgccgcacc cggcagaccg aacatcataa tcgggaagaa gccggacatg aacatccccg
     3241 cggtgccgtc accggcatag aagcggttga tatcaccgtg gaaaaccgta cccgccgcgt
     3301 tggtgaattc accaatctgg aaccaggcga tggtgttcag cacctgatgc agaccggttg
     3361 ggatcagcag acggttgatg aaaccaaaga taccggaacc cagcgcgccc gcagaaacga
     3421 tccactcgcc tcctgcatgg atagcgtgct gtaccggcgg ccagacgtaa ccaaaaatgg
     3481 ccgccagcac caggcagaag aagccggtgg caatcggcac aaagcgtttg ccgccgaaga
     3541 aactcaggaa gtccggcagt ttaatatcgg accaacggtt ataggctgcg ccaccaacca
     3601 gaccggtaat gatacccgcc agtacaccca tgttaatttc tgggttgatg gtcaccatcg
     3661 ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc cgccgcacct gcgctgtctt
     3721 tcgaccagct ggatgccaca ccgatggcga agattaatgc gaggttatca aaaatcgcac
     3781 cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca
     3841 gtgccgccac cggcagcacc gcgataggga gctgtaacgc cctaccgagt cgctggaaaa
     3901 aacctaaaat attcatctta ttccccctac gagaacccta tttggctcgt ttaaagccgt
     3961 atttttattt tgctgcaaat tgtactgccg atgttctgta atcagattgt tagatcatct
     4021 gctacagagt gtgtgaaaat ttaattcgta tcgcaaatta aacgcgtgtc ttttgtgagt
     4081 tttgtcacca aatatcgtta tcatcactcc cttttactgg ctaaagcaga aaacttattt
     4141 tatcattcaa aaaatcaggt cggattgacg cctgtctgcg caaatccagg ttacgcttaa
     4201 agatgcctaa tccgccaacg gcttacattt tacttattga ggtgaataat gagactgatc
     4261 cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc
     4321 aatgcgttca aaccgactgc cgatcgtccg tttgttctgg gcctgccgac tggcggcacg
     4381 ccgatgacca cctataaagc gttagtcgaa atgcataaag caggccaggt cagctttaag
     4441 cacgttgtca ccttcaacat ggacgaatat gtcggtctgc cgaaagagca tccggaaagc
     4501 tactacagct ttatgcaccg taatttcttc gatcacgttg atattccagc agaaaacatc
     4561 aaccttctca acggcaacgc cccggatatc gacgccgaat gccgccagta tgaagaaaaa
     4621 atctgttctt acggaaaaat tcatctgttt atgggcggtg taggtaacga cggtcatatt
     4681 gcatttaacg aaccggcgtc ttctctggct tctcgtactc gtatcaaaac cttgactcat
     4741 gacactcgcg tcgcgaactc tcgtttcttt gataacgatg tcaatcaggt gccaaaatat
     4801 gccctgactg tcggtgttgg tacattgctg gatgccgaag aagtgatgat tctggtgctg
     4861 ggtagccaga aagcactggc actgcaggct gcagttgaag gttgcgtgaa ccacatgtgg
     4921 accatcagct gtctgcaact gcatccaaaa gcgatcatgg tgtgcgatga accttccacc
     4981 atggagctga aagttaagac tttaagatat ttcaatgaat tagaagcaga aaatatcaaa
     5041 ggtctgtaat tgttatccct gccctggctc cttgctcagg gcaatatttt ttaaaatcgg
     5101 gggtcagaat gtatgcatta acccagggcc ggatctttac cggccacgaa tttcttgatg
     5161 accacgcggt tgttatcgct gatggcctga ttaaaagcgt ctgtccggta gcggaactgc
     5221 cgccagagat cgaacaacgt tcactgaacg gggccattct ctcccccggt tttatcgatg
     5281 tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg
     5341 aaacgctgga aatcatgcag aaagccaatg agaaatcagg ctgtactaac tatctgccga
     5401 cgcttatcac caccagcgat gagctgatga aacagggcgt gcgcgttatg cgcgagtacc
     5461 tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga aggtccgtgg ctgaatctgg
     5521 taaaaaaagg cacccataat ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt
     5581 tcctgtgtga aaacgccgac gtcattacca aagtgaccct ggcaccggaa atggttcctg
     5641 cggaagtcat cagcaaactg gcaaatgccg ggattgtggt ttctgccggt cactccaacg
     5701 cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat tacctttgcc acccatctgt
     5761 acaacgcgat gccgtatatt accggtcgtg aaccgggcct ggcgggcgcg atcctcgacg
     5821 aagctgacat ttattgcggt attattgctg atggcctgca tgttgattac gccaacattc
     5881 gtaacgctaa acgtctgaaa ggcgacaaac tgtgtctggt taccgacgcc accgcgccag
     5941 caggtgccaa cattgaacag ttcatttttg cgggtaaaac aatatactac cgtaacggac
     6001 tttgtgtgga tgagaacggt acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc
     6061 gtaatctggt cgaacattgc ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct
     6121 atccggcgcg tgcgattggc gttgagaaac gtctcggcac actcgccgca ggtaaagtag
     6181 ccaacctgac cgcattcaca cctgatttta aaatcaccaa gaccatcgtt aacggtaacg
     6241 aggtcgtaac tcaataagag aaagtatgac accaggcgga caagctcaga taggtaatgt
     6301 tgatctcgta aaacagctta acagcgcggc ggtttatcgc ctgattgacc agtacgggcc
     6361 aatctcgcgg attcagattg ccgagcaaag ccagcttgcc cccgccagcg taaccaaaat
     6421 tacgcgtcag cttatcgaac gcgggctgat caaagaagtt gatcagcagg cctccaccgg
     6481 gggccgccgc gctatctcca tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg
     6541 gcttggtcgt cacgacgcca ccatcaccct gtttgatctc agcagcaaag tgctggcaga
     6601 agaacattac ccgctgccgg aacgtaccca gcaaacgctg gaacatgccc tgttgaatgc
     6661 cattgctcag tttattgata gctaccagcg caaactgcgc gagctgatcg cgatttcggt
     6721 gatcctgcca gggcttgttg acccggacag cggcaaaatt cattacatgc cgcatattca
     6781 ggtagaaaac tgggggctgg tagaagcact ggaagaacgt tttaaagtga cctgtttcgt
     6841 tggtcacgat atccgtagtc tggcgctggc ggagcactac ttcggtgcaa gtcaggattg
     6901 cgaagactcc attctggtgc gtgtccatcg cggaaccggg gccgggatta tctctaacgg
     6961 gcgcattttt attggccgca acggcaacgt cggtgaaatt ggccatattc aggtcgaacc
     7021 gctgggtgaa cgctgccact gcggcaactt tggctgcctg gaaactatcg ctgccaacgc
     7081 cgctattgaa caacgggtgt tgaatctgtt aaagcagggc taccagagcc gcgtgccgct
     7141 ggacgactgc accatcaaaa ctatctgcaa agccgcgaac aaaggcgata gcctggcctc
     7201 ggaagtgatt gagtatgtcg gtcgtcatct gggcaaaacc atcgccattg ctatcaactt
     7261 atttaatccg caaaaaattg ttattgccgg tgaaatcacc gaagccgata aagtgctgct
     7321 ccctgctatt gaaagctgca ttaataccca ggcgctgaag gcgtttcgca ctaatctgcc
     7381 ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc gcttttgcgc tggtaaaacg
     7441 cgccatgctc aacggtattt tgctccagca tttgctggaa aattaatgtg cttttatagt
     7501 ggcgcttatt gttgtcaata ttctgggtag tccatgacca ttaaaaatgt aatttgcgat
     7561 atcgacggcg tgctgatgca cgataacgtc gccgtaccgg gtgcagcgga atttttgcac
     7621 gggattatgg ataaaggcct gccgctggtg ttgctgacca actatccttc gcagaccggg
     7681 caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg tgccggatag cgtgttttat
     7741 acctctgcga tggcgactgc cgatttcctg cgtcgccagg aaggcaagaa agcgtatgtg
     7801 gtgggcgaag gcgcactgat tcatgaactg tacaaagccg gtttcactat taccgatgtg
     7861 aaccctgatt ttgtgattgt tggcgaaacg cgttcctaca actgggacat gatgcataaa
     7921 gcggcctatt tcgtcgctaa cggtgcacgc tttatcgcca ccaacccgga cacccacggg
     7981 cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga ttgagaaaat ctccgggcgc
     8041 aaaccgttct atgttggtaa gcccagcccg tggatcatcc gcgcggcatt aaacaaaatg
     8101 caggcgcatt cggaagaaac ggtgattgtc ggcgataact tgcgcaccga cattctggcg
     8161 ggcttccagg cgggtctgga gacgattctg gtgctttctg gtgtttcatc gctcgacgat
     8221 atcgacagta tgccgttccg cccaagctgg atttacccgt cggtcgctga aatcgacgtt
     8281 atctgaatag caaccacgtc tcagggcgtg gtttcttatt tttacccatc attaaaatca
     8341 tcccattcaa taaaactcgc caaaaattat cgatccatca ataaatacat caccacaccc
     8401 tccccttttt cacaaatcag caatcaccat tacgtttttt atttttgcac tgccaaatcg
     8461 cttgcgcacg gtgctgcttt acggcatttt tttaaacaag caaacacaac aagcaacaaa
     8521 taccaggtta acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga
     8581 cgcagttgag ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc
     8641 ggactggtcc ggtatttatg ccagcgataa cgccattctc gcccacgaac gtctgtcaat
     8701 tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa caaaaaactc atgtgctggc
     8761 ggtaaacggt gaaatctaca accaccaggc actgcgcgcc gaatatggcg atcgttatca
     8821 gttccagacc ggatctgact gcgaagtgat cctcgcgctg tatcaggaaa aagggccgga
     8881 atttctcgac gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc
     8941 atatctgatt ggtcgcgacc atctggggat catcccactg tatatgggct atgacgagca
     9001 cggtcagctg tatgtggcct cagaaatgaa agccctggtg ccagtttgcc gcacgattaa
     9061 agagttcccg gcggggagct atttgtggag tcaggacggc gaaatccgtt cttattatca
     9121 tcgcgactgg ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg
     9181 tcaggcactg gaagattccg ttaaaagcca tctgatgtct gacgttcctt acggtgtgct
     9241 gctctcgggt ggtctggatt cctcaattat ttccgcgatc accaagaaat acgccgcccg
     9301 tcgcgtggaa gatcaggaac gctctgaagc ctggtggccg caattacact cctttgctgt
     9361 aggtctgccg ggttcaccgg atctgaaagc agcccaggaa gtggcaaacc atctgggcac
     9421 ggtgcatcac gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat
     9481 ttaccacatc gaaacctatg atgtgacaac tattcgcgct tcaacaccga tgtatttaat
     9541 gtcgcgtaag atcaaggcaa tgggcattaa aatggtgctg tccggtgaag gttctgatga
     9601 agtgtttggc ggttatcttt acttccacaa agcaccgaat gccaaagaac tgcatgaaga
     9661 gacggtgcgt aaactgctgg ccctgcatat gtatgactgc gcgcgcgcca acaaagcgat
     9721 gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc
     9781 gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aacacatcct
     9841 gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt
     9901 ctccgatggc gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt
     9961 ttctgatcag caactggaaa ctgcccgctt ccgcttcccg tacaacacgc caacctcaaa
    10021 agaagcgtat ctgtaccggg agatctttga agaactgttc ccgcttccga gcgccgctga
    10081 gtgcgtgcct ggcggtcctt ccgtcgcgtg ttcttccgct aaagcgattg agtgggatga
    10141 ggcgttcaag aaaatggacg atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta
    10201 taagtaagaa atgcgaaaca gccccggtga aatccggggc tgtttcagtt attgaggtgc
    10261 ttgttaattc tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa
    10321 ctcatcactg taggccagta tttcatagat taatttccca ccactattgt tgaattccga
    10381 atttcatcct gtacgacaaa agaatggctg tagggtgggg aaaaaacaca aagtaaacaa
    10441 taattgacga atatagcgcc acgctgttcg caacctaacc aaacagtcac tttcgagcaa
    10501 ttttccttga aaaagaggtt gacgctgcaa ggctctatac gcataatgcg ccccgcaacg
    10561 ccgataaggt atcgcgaaaa aaaagatggc tacgtagctc agttggttag agcacatcac
    10621 tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accatctttt ttgcgggagt
    10681 ggcgaaattg gtagacgcac cagatttagg ttctggcgcc gcaaggtgtg cgagttcaag
    10741 tctcgcctcc cgcaccattc accagaaagc gttgtacgga tggggta
//
LOCUS       DAZFWP020000161        10774 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFWP020000161 DAZFWP020000000
VERSION     DAZFWP020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.161"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            126..839
                     /gene="yghS"
                     /locus_tag="ACHK0Z_RS20410"
                     /old_locus_tag="pgaptmp_004082"
     CDS             126..839
                     /gene="yghS"
                     /locus_tag="ACHK0Z_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4504012.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            871..1628
                     /locus_tag="ACHK0Z_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /pseudo
     CDS             871..1628
                     /locus_tag="ACHK0Z_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1674..3023
                     /locus_tag="ACHK0Z_RS20420"
                     /old_locus_tag="pgaptmp_004084"
     CDS             1674..3023
                     /locus_tag="ACHK0Z_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGU4504013.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3023..3847
                     /locus_tag="ACHK0Z_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             3023..3847
                     /locus_tag="ACHK0Z_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504014.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLIRKQTKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3859..4419
                     /locus_tag="ACHK0Z_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             3859..4419
                     /locus_tag="ACHK0Z_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4504015.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4450..5520
                     /gene="lptF"
                     /locus_tag="ACHK0Z_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             4450..5520
                     /gene="lptF"
                     /locus_tag="ACHK0Z_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4504016.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALYVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5517..6596
                     /gene="lptG"
                     /locus_tag="ACHK0Z_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             5517..6596
                     /gene="lptG"
                     /locus_tag="ACHK0Z_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4504017.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6632..7804)
                     /locus_tag="ACHK0Z_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             complement(6632..7804)
                     /locus_tag="ACHK0Z_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4504018.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7804..8052)
                     /locus_tag="ACHK0Z_RS20450"
                     /old_locus_tag="pgaptmp_004090"
     CDS             complement(7804..8052)
                     /locus_tag="ACHK0Z_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4504019.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8084..8998)
                     /locus_tag="ACHK0Z_RS20455"
                     /old_locus_tag="pgaptmp_004091"
     CDS             complement(8084..8998)
                     /locus_tag="ACHK0Z_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4504020.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8995..10716)
                     /locus_tag="ACHK0Z_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             complement(8995..10716)
                     /locus_tag="ACHK0Z_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGU4504021.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKAL
                     PEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRC
                     VSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPF
                     GYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCY
                     GLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHG
                     IEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGY
                     LYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISD
                     EERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASL
                     HVQESLA"
BASE COUNT         2592 a         2684 c         2822 g         2676 t
ORIGIN      
        1 atatgttttt atgaaagcgt aattcaggtc aatgtcacaa ttaaccattg tcacaataag
       61 gttgaacgga tattctgagc acggaccacg taatattcaa tacattattt actgccgggt
      121 ttttcgtgaa tcaacgtaat atgtcaataa ttaattccac gccagtgcgt gttattgcca
      181 ttgtaggatg tgatggttca ggtaaatcga ccctcacggc aagcctggta aatgaactgg
      241 cagcaagaat gccaacagaa cacatttatc tcgggcaatc gtccgggcga attggcgaat
      301 ggatttcaca gctccctgtt attggcgcac cttgtgggcg ttatctgcga agtaaagcgg
      361 cacatgtgca cgaaaaaccc tcaacaccgc ctggcaatat tactgcgctg gttatctatc
      421 tgctttcctg ctggcgggcg tacaagtttc gcaaaatgtt gtgtaaaagc cagcaaggct
      481 ttctgctcat caccgaccgc tacccgcagg ttgaagtgcc gggttttcgc tttgatggcc
      541 cgcaattggc aaaaaccacg ggcggtaacg gttggataaa aatgttaagg cagcgcgagc
      601 tgaagctgta ccaatggatg gcatcttatt tgcccgtatt gttgattcgt cttggcattg
      661 atgaacaaac cgcgtttgcg cgtaaacctg accaccaact ggcagcgtta caggaaaaaa
      721 tcgccgttac gccgcaactg acattcaatg gcgcaaagat ccttgaactg gatgggcgac
      781 accccgccga tgaaattctg caagcgtcac tacgcgcaat tcacgccgcg ctttcctgat
      841 caccaaaatg gataggggat gatttaacga atggatgcac tacaaactca aactgttaat
      901 agtacaaccg cgccgcagcc caactacatt ccggggctga ttgcggtggt cgggtgtgat
      961 ggcaccggta aatccacact gaccaccgac ctggtgaaat cgctgcaaca acactggcaa
     1021 accgagcggc gctatctggg gctgctctcc ggcgaagacg gcgacaaaat caaacgattg
     1081 ccgttggttg gcgtctggct ggaacggcga ctggcggcca aatcctcgaa aacccaaagc
     1141 atgaaaacca aatctccggc gctatgggcg gcggtgatta tgtactgctt ctcgctgcga
     1201 agaatggcga atctacgcaa ggttcagcga ctggcgcaaa gtggcgttct ggtggtcagc
     1261 gatcgcttcc cgcaggctga aatttcgggc ttttattatg atggaccggg gattggcgtc
     1321 gaacgtgcga ccgggaaaat cagcatgttt ctggcgcagc gcgaacggcg tttataccaa
     1381 caaatggcgc aatatcgccc ggaattaatt attcgcctgg gcattgatat tgagactgcc
     1441 atctcccgca agcctgacca tgactatgcc gagctgcagg acaaatcggc gtcatgtcga
     1501 cgattggcta taacggcaca aaaattcttg agattgattc cagagcgccc tacagcgaag
     1561 tgctggaaca ggcacagaag gcggtttctc tggttgccat cgtttctgac cgccgaagtt
     1621 taacttaagg ctggcgggat actttttact atcaacgata aggtcaggct gaattggcgg
     1681 gttttaacat caaacattgg tttgcagatg gcgcgtttcg caccattatt cgcaatagcg
     1741 cctggttagg ctccagtaat gtcgtgagcg ccttgctggg tctgttggcg ctctcgtgtg
     1801 ccggtaaagg gatgacgccc gccatgtttg gcgtactggt gattgtgcaa tcgtacgcca
     1861 agtcgatcag cgattttatt aagtttcaga catggcaact ggtggttcag tacggaacgc
     1921 cagccttgac caacaataat ccgcagcaat ttcgcaatgt cgtctcattt tccttctcgc
     1981 tggatatcgt cagcggcgcg gtggcgattg tcggtggcat tgccttactg ccattccttt
     2041 cccattcatt aggtctggat gaccaaagct tttggctggc ggcgctctat tgcacgctca
     2101 ttccttcaat ggcttcctcc acgccgaccg gcattctgcg tgcggtagat cgcttcgatt
     2161 taattgctgt acagcaggcg acgaaacctt ttctgcgcgc agcggggagc gtcgtagcct
     2221 ggtattttga ctttggtttt gcgggttttg ttattgcctg gtacgtgtcg aatctggttg
     2281 gcggcaccat gtactggtgg tttgcagcgc gcgaattacg ccgcagaaat atccataatg
     2341 ccttcaaatt gaatctgttt gagtctgccc gacacatcaa aggcgcgtgg agttttgtct
     2401 ggtcaaccaa cattgcccac tccatctggt cggcgcgtaa ctcgtgcagc acagtgctgg
     2461 tggggatcgt gttaggaccc gctgccgccg ggttatttaa aatcgccatg acattcttcg
     2521 acgccgccgg aacgccagcg ggtttgctgg gtaaaagttt ttacccagag gtgatgcgtt
     2581 tagatccgcg caccaccaga ccgtggttgt taggtgtgaa gtccggttta ctggcgggcg
     2641 gaatcggtat tctggtggcg ctcgcggtgt tgatcgtcgg caagccgctc atttcgcttg
     2701 tgtttggcgt taagtatctc gaagcgtatg acctgattca ggtgatgctg ggcgcaattg
     2761 tgatttcgat gctgggtttc ccgcaggagt cattgctatt gatggcggga aaacagcgcg
     2821 cttttctcgt ggcgcaaacc atcgcctcaa tcggctacat cgtattgctg ttcatgttct
     2881 gtcatctgtt tggtgtgctg ggagcagcgt ttgcttactt cggtgggcaa tgtctggatg
     2941 tggtgctctc gttaattcca actttaaagg cattttttca gcgtcattcc ttgctttata
     3001 acgcagccgg agagaaatcc tgatgatgag aaaatatttc ccgcttgagg cgagcgagcg
     3061 tctatttgtt gcgattgagg aagatgatgt cgttgatgcc caggtcagtt tgccgccgac
     3121 tattgcacta agttgtacca ccgaaatcat ccatgataat tacgcgttat gcctgcagtt
     3181 ctggctcaat ggcgttaatc gtcaggagtt actgcgttta attcgcaaac agaccaaagg
     3241 cgatgagctg acgacagacg agcgcaaaca gtttaaatac atgcgagcgc gctacaagca
     3301 cctgcgtttt gcgcaacggc tgtatttaaa gaagcatcag gccggatttt tattcggcaa
     3361 aacgaccgtt tttctggggc gttttcagga cggctttcgt aacggtaaga aaaatattgt
     3421 gtcgtattac ggcaacctgt tacggattta tttaagctct ccggtctggt cgttggtgaa
     3481 ctattcatta cgccatagcc agttagaaag cgtaagcggt tttattgcct atcggcaaaa
     3541 gcaaatgcat accctgaaag agattattgc caaaccgcga ctaaccggta gggaatttca
     3601 tgatgtgcga aaaatcatca gccagcaggt ttcttattac gacaccttaa gatcgcttga
     3661 tcctgaaaat aaagaggcgt tacagatatc ccgcttcctc gccgccatta atggcctgat
     3721 gggagataaa cacgacgaca tggtggcgga tgatatggaa aaccgccagt cgtatgatgc
     3781 accggtggcg ctggatagcg atattcgcca gcgcttagag ctgctgattt cgcgttttcc
     3841 gctataaccg gagcaatgat gtcatctcgt ttgattattg cgctgattat catgctgtta
     3901 gccccgggtg tgcaggcaca caatttcgtc actggcaaaa cggttacccc tgtttatatc
     3961 caggaaggcg gcgagctgct tctgaatagc gacgacgaaa ttcactacca aaagtggaac
     4021 agcacacaat tggcgggaaa agtccgcatt atccagtata tcgccgggcg taagtcggcg
     4081 aagaaaaaga actcactgtt aattaaagcg gttgaagcgg caaactttcc gcaggatcgt
     4141 tttcagccca ccaccattgt gaacaccgat gatgccatct ttggtactgg ctattttgtt
     4201 gttggcaaaa tcgaaaaaaa taaacgccgc tatccgtggg cgcaattcgt tattgacggc
     4261 aatggacagg gacgcgttgc ctggcgcttg ccggagcaga gttcaaccat tctggtgctg
     4321 aataaagccg ggcaaattca gtgggcaaag gacggcagcc tgacgccgga agaggtagat
     4381 catgtcattg cgttggcgca gaaactgatc aatgaataaa aaactcaaag gattgagggc
     4441 gttagtgaga tgaagctggt tgaacactac atcatgcgtg gtacgcgccg tctggtgctg
     4501 attatcgtcg gttttctgat ctttattttc gccagctact ccgcacagcg ttatctgacc
     4561 gaagcggcaa atgggacatt agctctttat gttgtgctgg atatcgtttt ctacaaagta
     4621 ctgattgcac tggagatgtt gttacctgtt ggtctgtatg tgtcagttgg cgtgacgcta
     4681 gggcagatgt ataccgactc ggaaattacc gctatctctg cggcgggggg cagtccggga
     4741 cgcttgtaca aagccgttct ttatctggcg ataccgctaa gtatttttgt cacccttctg
     4801 tcgatgtatg gtcgtccgtg ggcttatgcg cagatttatc aactggagca acagtcgcag
     4861 tcggagctgg atgttcgcca gttgcgggca aagaaattta acactaacga taacggacga
     4921 atgatccttt cgcagacggt tgatcaggat aataatcgcc tgactgacgc gctgatttat
     4981 acttctactg ccaatcgaac ccgtattttc cgcgcccgtt cggttgatgt ggttgaccca
     5041 tcacccgaga aaccgaccgt tatgttgcac aacggcactg cctatcttct cgatcatcaa
     5101 gggcgtgacg acaacgaaca gatctaccgt aatctgcaat tacatctgaa tccgctggat
     5161 caaagcccga acgtcaaacg caaagcgaaa tcggtcacgg agctggcgcg ctccgtcttt
     5221 cctgccgatc atgccgaact gcaatggcga caaagccgtg gtctgacagc attgttgatg
     5281 gcgctgctgg ccatttcatt aagtcgggta aaaccgcggc aagggcgatt ttcaacgtta
     5341 ttgccactga cgttgctgtt tattgccatt ttttatggcg gcgacgtctg ccgtacgctg
     5401 gtggctaacg gcgcgattcc cctcattcct ggtttgtggt tagtgcccgg actcatgcta
     5461 atgggcctgc tgatgctggt cgcacgcgac ttctctttgc tgcagaaatt ttcccgatga
     5521 atgttttcag tcgctattta atccgtcatc tctttctcgg ttttgccgcc gccgcagggc
     5581 tattgctgcc gctttttacc accttcaact tgattaacga actggatgat gtcagcccgg
     5641 gcggttatcg ctggactcag gcggtgctgg tggtgctaat gaccttaccg cgcacactgg
     5701 tcgaactttc gccgtttatc gcgttattgg gtggtattgt tggactgggg cagttatcga
     5761 aaaacagtga gcttaccgcc attcgcagca cggggttttc tatcttccgt attgcactgg
     5821 tggcgctggt tgccgggata ttgtggactg tttcgttagg cgcgatagat gagtgggtgg
     5881 cgtcgccatt gcagcaacag gcgttgcaaa tcaaatcgac cgccaccgcg ttgggggagg
     5941 acgatgacat taccggcaat atgctgtggg ccaggcgcgg taatgaattt gtgacggtga
     6001 aatcgctgaa cgagcagggc cagccggtgg gcgtggagat atttcattat cgcgacgatc
     6061 tctcgctcga atcttacatt tatgcacgca gtgccaccat tgaagacgac aaaacgtgga
     6121 tcctgcatgg tgtgaatcat aaaaaatggc ttaacggtaa agaaacgctg gaaacatcag
     6181 ataatcttgc ctggcaatcg gccttcacca gtatggatct tgatgagtta tcgatgccgg
     6241 ggaatacttt ttctgtccgt cagcttaatc attacatcca ttatttgcag gaaaccgggc
     6301 aacccagcag cgaataccgc cttgcactgt gggaaaaact ggggcaaccg atcctgaccc
     6361 tggcgatgat tttgctggct gtaccgttta cctttagcgc cccgcgctcg ccagggatgg
     6421 gtagccgtct cgctgtaggc gtcatcgtgg gcttactcac ctggatcagc tatcaaatca
     6481 tggttaatct gggattgcta tttgcgttaa gcgcacctgt taccgcgctc ggtttaccgg
     6541 tagcgtttgt gttggtggcg ttgagcctgg tgtattggta tgacagacaa cattaaaccc
     6601 gccgtggcgc aaacaccacg gcgggctcac attactgctt cagagtagca aatgcctgaa
     6661 tgatggcatc gatttgctca cgactgtggg cagcatttac gctacagcgc agcagcgtga
     6721 tgcctgcagg cgcggcaggc ggcaggataa gattcacata cacgcccagc gagataagct
     6781 cgcgccagat gcgtaaacct tcctctttcg aaccgattat tactggcact accgggctaa
     6841 tatgtgaact taactcataa cccagttttt gcagaccgtc atacagatgg ttggcgttat
     6901 ccatcagctt ttgccgcagc tctggttgtg tggcaatagt tcttaacgac gagcgcacgg
     6961 tcgcaatgca ggacggagaa ggagaggcgg taaaaatata cgggcggctg ccgtagcgca
     7021 gcacttccat cgcttccgaa ccgacagcaa aaccgccaat cgaggccaga cttttactga
     7081 aagtaccaac gataatatcc acatcatctt caacgccgac agcttcagcc agtccgcgcc
     7141 ccgttgcgcc taacacgcca aacgaatgcg cttcatcgac aatcaaataa ccaccgagac
     7201 gacgtttgat atcaacaatt tccgccagcg gcgcaacgtc accgagcatg ctgtagatgc
     7261 cctcaacgat gataatcgcc tctttcgccc gttcaccgag gcgaaccatg cgccgttcca
     7321 gatctttagc atcgttatgg cggaaacgaa taatttccgc gccaccgaga gaacaggcgt
     7381 cataaatact ggcgtggctg tcggcatcca gtaacaccac ggcgttatgg tcggcaagtg
     7441 cgctgatgac gcctaaatta gcggtataac cggttgaaaa aacgatagcg gtagggcgat
     7501 tgaaaaactc ggcaatctct ttctctaacg ccaggtgcgg cgcataactg ccgtttgcca
     7561 tacgagaacc ggtggtgccg gtgccttggg ccgctaacgc tgcctgacct tcgctgatgg
     7621 catcatgatt aaaggttaaa ccgagataat tattggtccc ggccagaatc acttttttat
     7681 tgccgatccg gccttcggtg gcggaataca cttcatcaat acaggtgcca aacggtgtta
     7741 acccgttgtc actaaattgc agtcgctcgc cagcaaggcg cgcatattta tcgtatagcc
     7801 ccattattta ttctccagcc atggaagtag agtttccatt aattgcgcag gggttttgac
     7861 atccagcaga atattaatag gaatggaaat atcgaatctg tcttccagca tcattaagag
     7921 atccatcact ttgatcgact ccaggccaag atcgttgacg agatcgctgt cgggcttaat
     7981 ttccacaccg ttttcgacca aatcctgtaa acaggaaagg atgtaatcca ttactatttc
     8041 acgatttacc atagaaacgt taacacacct ttaaacaaga caatcaaaat agcccgttgc
     8101 gtaatgcgtg ttccaggcta atcccgggaa accaattaat atcttcagaa atacggttat
     8161 tacttgccga ccagtcggcg tgggttaatt cacgaatttt cgagcgggtc agcataggtt
     8221 ctttacctgc caggcgactc aacgcggtac tgatatccgc aaggcaggtg agtaccggca
     8281 gaggaatacc aaccattcgc acggaaccgc aacggacgtt ggcggcaagt tgctgtatgc
     8341 gttgccagtc atagccgcca gcgacgccat cgcataattc ataggtttgc gtctgtatag
     8401 tttcggcgct taaccactga cccactgctt gcgcgaaatc ggtgacgtgc agaaaagaga
     8461 gctgtgtatc tggcgcacca agtcgtggca gcaggccgcg cagcatccag tcaaacagcg
     8521 gttttaactc tttatcgccg ggaccatata cggctgtcgg gcgaaaaacg cccagcgtaa
     8581 tttcgtcagc cattgcagtc agccgttgtt cggcgacgtg tttggaattt gcgtaccagg
     8641 agagttcggg atggcgcgcc gccagcgaag agataaacag aaaacgttgg caaaaaccgc
     8701 tctcttttgc tgcttgcatc aggcgcaggc tgccgtcaac gttacagcgg gtgaaaatct
     8761 cttctttgtg cccgcgcact tgcccggcgc aatggaccac cgcgctggct ccggcaacca
     8821 gcttgctaag tgaatgagta tcttccagcg aaccgcgcac ccaggtgaga ttatcattga
     8881 cgtgagcgcg ggcagtacgc gtcaatgcgc gaacgtgaaa gccgcgggcg agcaggttat
     8941 cgataatata tttaccgata aacccggtag cgcccgtcac cgcgacagtt tgattcatgc
     9001 cagggattcc tgcacatgaa ggcttgcagc ataagccttc tgataacgtt ttttcgcttc
     9061 cgcacgggca ggcttgccgg aggatgttcg gggaatactg tggggcggca acagatcgat
     9121 agccgctgtt acgccaaatt cgctttggat ccgtgctgcc agcgcgtgga taagctgccc
     9181 gcgacgttct tcgtcgctga tccgacactg gatctgcaaa atgatttttt cctgggcggt
     9241 aacaaaagca atcgcatcgc cagaatgaat ttccggttct tgttccgcaa tatattcaat
     9301 atcctgcggc cagatattac ggccacgaat aataatcaga tctttaatac gtccggtgac
     9361 atacagataa ccgtccagca gataaccgag gtcgccggtg tctaaccagc ccgtcgcggc
     9421 aatctcgtct tgcgaaacct ggtcgccaaa gtaaccgctc atcaggctgg ggccggagat
     9481 gcaaatatgg cctaccacac gttccgcgac cggcatacct gcttcattgc ggatttcaat
     9541 gccatgttcc ggcaacgctt tgccgcagtt gacgaaagtc gatacggcgc gtgtttcagc
     9601 acccggcgcg acggctttgc cctgatattc taggatgtcg cgatccactt cgttaaccac
     9661 aaccccagag ccttcatcag agaagctgac agccagcgca ttttctgcca gtccgtagca
     9721 cggcatgaac gttttatcgt caaagttaac ctgacggaaa cattcagcga attgatggag
     9781 ttgttctgcg gagatcggct cagcaccaat accagcgacg cgccagcagg aaagatccag
     9841 ttcagcgaga tctttttcat tcacgcggcg ctggcacaat tcatagccaa acggcggcgc
     9901 aacggaaacg gtgccgcgat ttttactgat caatttaagc cattgcagag gacgcatggc
     9961 aaaatcctga gtgcgcaaat aatctactga aagttgcgtg gcgacggggg tcaggagaaa
    10021 gccgaccagt cccatatcat ggtagaaagg cagccaggag acgcagcggt cgccagggcg
    10081 taatttaatg ccatcgtggc ttatagcacg tagattagcc atcacttcgc gatgggtgat
    10141 aatgacgcca cggggaaaac gggtgctgcc ggaggtgtac tggaggtagg cgatatcgtt
    10201 cggaactgga cgctggagcg caacatcggc ttccggtaat gccttaaacc aggcgtggct
    10261 taaaacatgt aattcggggt tgttatgcgt cgcggcattg accagtggca accactcatc
    10321 accagtgata atggctgcgg gctggcaact tgccagtaaa ccctggagtt tggcgctcca
    10381 ggaatcccgc tgaccgacgc ccattggaat cgccaacggg acggcgacta agccagcata
    10441 ctgacaggca aaaaaagcct ctacaaaccc gctactcgtt tcggcaatca gcgccacgcg
    10501 ttcgcctttt ttcaggttca gcgataacaa ccgcttcgca ccagcttcgg cacgcgtttt
    10561 taacgtctga tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc
    10621 ggcgctactc agagcggcgt agtccaaagc atcaaccagc gttggaaaat cggcatagcg
    10681 cataggtagg gaatgcgtaa agattttatt agacatatac accacgaaaa aattggatat
    10741 caaaaataaa atgacaataa tgcggtggat tata
//
LOCUS       DAZFWP020000162        10770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFWP020000162 DAZFWP020000000
VERSION     DAZFWP020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.162"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            122..1264
                     /gene="yghO"
                     /locus_tag="ACHK0Z_RS20465"
                     /old_locus_tag="pgaptmp_004093"
     CDS             122..1264
                     /gene="yghO"
                     /locus_tag="ACHK0Z_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HGU4504022.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGVEPYKRYRLYEKQI"
     gene            complement(1271..2035)
                     /gene="glcC"
                     /locus_tag="ACHK0Z_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             complement(1271..2035)
                     /gene="glcC"
                     /locus_tag="ACHK0Z_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGU4504023.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTNLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2286..3785
                     /gene="glcD"
                     /locus_tag="ACHK0Z_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             2286..3785
                     /gene="glcD"
                     /locus_tag="ACHK0Z_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HGU4504024.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3785..4837
                     /gene="glcE"
                     /locus_tag="ACHK0Z_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             3785..4837
                     /gene="glcE"
                     /locus_tag="ACHK0Z_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGU4504025.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEL
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4848..6071
                     /gene="glcF"
                     /locus_tag="ACHK0Z_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             4848..6071
                     /gene="glcF"
                     /locus_tag="ACHK0Z_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HGU4504026.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6076..6480
                     /locus_tag="ACHK0Z_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             6076..6480
                     /locus_tag="ACHK0Z_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGU4504027.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6502..8673
                     /gene="glcB"
                     /locus_tag="ACHK0Z_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     CDS             6502..8673
                     /gene="glcB"
                     /locus_tag="ACHK0Z_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGU4504028.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMDKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9028..10710
                     /gene="glcA"
                     /locus_tag="ACHK0Z_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             9028..10710
                     /gene="glcA"
                     /locus_tag="ACHK0Z_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HGU4504029.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2497 a         2713 c         3030 g         2530 t
ORIGIN      
        1 tcataatcat taataaatac tatttattct ttgtttgtat tcattgggtt gattattaag
       61 gtgtcgttcg ttgctgttgc aacctgtttt tcaccgttaa ttatttgtta tccacagaga
      121 tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa atgaccttaa
      181 ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga tacccccttt
      241 attcatagaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc atatcatctg
      301 gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg cgcaaataga
      361 taccttgcac cgtgagcgtt acggtgaaga taccggtcat ttcggcatga ttgacgccat
      421 tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt tgaagtcaca
      481 aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa gcggattact
      541 gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac cgtggtatgc
      601 cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt ggtggatgca
      661 gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg tgcgcaaaaa
      721 ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gaaatgcaga tcctgcgtga
      781 gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg aacatgaatt
      841 cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct atatcgctga
      901 gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg aggcgattgc
      961 cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt
     1021 cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt atcagttcag
     1081 ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg
     1141 ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag gcatgaataa
     1201 catgctggaa cgcattgggg tggagccgta caagcgttac cgattgtatg aaaagcagat
     1261 ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc
     1321 cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg cgtgctgcgc
     1381 gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca tgctggcgat
     1441 cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca aacactgaat
     1501 taaacatcag attggtcaat gattgcagcg taaacaccag cacctgattg tgagaagcct
     1561 gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc gaaatctctt
     1621 tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca acaaaatcag
     1681 cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt
     1741 cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc gggctggtgt
     1801 cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt tcaataatcc
     1861 cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag ccgagctttt
     1921 cacacagtcg acgttccgag ggaagtggct gaccgacctt cagtacgccg tcgataatta
     1981 accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct ttcattcccg
     2041 gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac
     2101 gtaagtgatt atgttacatc aatttaacat tgagttaacc aaggcaaggt cacagagctg
     2161 gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag cccgcttaat
     2221 aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca aagcgaagcc
     2281 tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat gtcgaccgca
     2341 catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg cataccgatg
     2401 aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt ccattactgg
     2461 ttgttctgcc taagcaaatg gaacaggtga cagcgattct tgctgtctgc catcgcctgc
     2521 gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg ctgccgctgg
     2581 aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt aaccccgttg
     2641 gtcgccgcgc gcgcgtgcag ccaggcgtgc gtaacctggc gatctcccag gccgttgcac
     2701 cgcataatct ctactacgca ccggaccctt cctcacaaat cgcctgttcc attggcggca
     2761 acgtggcaga aaatgccggc ggcgtccact gcctgaaata tggtctgacc gtacataacc
     2821 tgctgaaaat tgaagtgcaa acgctggacg gcgaggcgct gacgctggga tcggacgcgc
     2881 tggattcacc tggttttgac ctgctggcgc tgttcaccgg atcggaaggt atgctcggcg
     2941 tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg gttctgttag
     3001 ccagctttga ctcggtagaa aaagccggac ttgcagttgg tgacatcatc gccaatggca
     3061 ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcggcg gaagatttta
     3121 ttcatgccgg ttatcccgtc gacgccgaag caattttgtt atgcgagctg gacggcgtgg
     3181 agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa gcgggcgcga
     3241 ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc ggtcgcaaaa
     3301 atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat ggcaccatcc
     3361 cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag caatatgatt
     3421 tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta atccttttcg
     3481 atgccaacga acccggtgaa tttgcccgcg cggaagagct gggtgggaag atcctcgaac
     3541 tctgcgttga agttggcggc agcatcagtg gggaacatgg catcgggcga gaaaaaatca
     3601 atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg gtcaaggcgg
     3661 cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta caccgctgtg
     3721 ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa ctggagcgtt
     3781 tctgatgcta cgcgagtgtg attacagcca ggcgctgctg gagcaggtga atcaggcaat
     3841 tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt taggtcgccc
     3901 tgtcaccggg caaacgctgg atgtgcgttg tcatcgcggc attgttaatt acgacccgac
     3961 cgagctggtg ataaccgcgc gtgccggaac gccgctggtg gcgattgaag cggcgctgga
     4021 gagcgccggg caaatgctcc cctgcgagcc gccgcattat ggtgaagaag ccacctgggg
     4081 cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt cggtccgcga
     4141 ttttgtcctc ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt ttggtggcga
     4201 agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa gctacggttg
     4261 tcttggcgtg ctcactgaaa tctcgatgaa agtgttaccg cgaccgcgcg cctccctgag
     4321 cctgcgtcgg gaaatcagcc tgcaagaagc catgaacgaa atcgcccagt ggcaactcca
     4381 gccgctaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc gccttgaggg
     4441 cggcgaagga tcggtaaaag cggcgcgtga actgctgggg ggcgaagagg ttgcgggtca
     4501 gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag gtaccttatg
     4561 gcgcatttca ttacccagcg atgcgccgat gatggattta cccggcgagc tactgatcga
     4621 ctggggcgga gcgttacgct ggctgaaatc gacagccgag gacaatcaaa tccatcgcat
     4681 cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg gctttgcccc
     4741 gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg acccttgcgg
     4801 cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg caaacccaat
     4861 taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc ctgcgcgcct
     4921 gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg ggcgatgaac
     4981 tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc aacgaagtca
     5041 cgcttaaaac acaggagcat ctcgatcgct gcctcacttg ccgcaattgt gaaaccacct
     5101 gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt gtcgagcaga
     5161 aagtaaaacg cccactgccg gagcgaatac tgcgcgaagg attgcgccag gtagtgccgc
     5221 gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg tttttaccgg
     5281 aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt ccgccgctgc
     5341 gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt tcgcccaaca
     5401 ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg tcagctaacg
     5461 aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa gggctggcac
     5521 gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgcc gaggcaattt
     5581 tgcaaaccgc cagcggttgc ggcgcgtttg tcaaagagta cgggcagatg ctgaaaaacg
     5641 atgcgttata tgccgataaa gcgcgtcaag tcagtgaact ggcggtcgat ttagtcgagc
     5701 ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg cgataaaaaa ctggccttcc
     5761 actgcccgtg taccctgcaa catgcgcaaa agctgaacgg cgaagtggaa aaagtgttgc
     5821 ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc ggttcagcgg
     5881 gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac aaaatgaatg
     5941 cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc cagacgcatc
     6001 tggcgagcgc cggtcgtacc tctgtgcgtc actggattga aattgtagaa caagcccttg
     6061 aaaaggaata aaaaaatgaa aactaaagtc attcttagcc agcaaatggc gagtgcaatt
     6121 attgccgcag gtcaggaaga ggcgcagaaa aataactggt ctgtttccat tgctgttgcc
     6181 gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc gattgcggct
     6241 tacatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac taagggctat
     6301 gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttact gacgtcgctg
     6361 gaaggtggcg taccggttgt tgttgatggg caaattattg gtgccgtggg cgtttctggt
     6421 ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt ggcgaaataa
     6481 gcgaaaacga ggagataaac aatgagtcaa accataaccc agggccgttt acgcattgac
     6541 gccaatttta aacgttttgt ggatgaagaa gttttaccgg gaacagggct ggacgctgcg
     6601 gcgttctggc gcaattttga tgaaatcgtt cacgatctgg caccagaaaa tcgtcagttg
     6661 ctggcagagc gcgatcgcat tcaggcggcg cttgatgagt ggcatcgcag caatccgggg
     6721 ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta cctggtgccg
     6781 caaccggagc gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac cagtcaggcg
     6841 gggccacagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc ggcgaacgct
     6901 cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca ggaaggggcg
     6961 atggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt tcggcgtttc
     7021 ctcgatgaat ctctaccgct ggaaaacggc agctatcagg atgtggtggc gtttaaggtg
     7081 gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt acgtaccccg
     7141 gcgcagtttg tcggttaccg tggcgatgcc gctgcgccga cctgcatttt gctgaaaaat
     7201 aacggcctgc atattgaact gcaaattgat gccaacgggc ggattggcaa agacgatccg
     7261 gcgcacatca acgatgttat cgtcgaagcg gccatcagta ccattctcga ctgcgaagat
     7321 tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct gctgggcctg
     7381 atgcagggga ctctgcaaga gaaaatggat aaaaacggtc ggcaaatcgt gcgtaaactg
     7441 aatgatgatc gtcagtacac cgccgccgat ggctctgaaa tttctctgca cggacgctcg
     7501 ttgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg ggacagcgaa
     7561 ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat tgccctctat
     7621 gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa accgaaaatg
     7681 cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga gacaatgctc
     7741 ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg gacctcgctg
     7801 aacttgcgta gctgtatcgc tcaggcgcgt aaccgcgtgg cgttcatcaa taccggtttc
     7861 ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat gctgcgtaaa
     7921 aaccagatga aatcgacgcc ttggatcaaa gcctacgaac gtaataacgt gctttccggc
     7981 ctgttctgtg ggctgcgcgg taaagcgcaa atcggtaaag gcatgtgggc aatgccggac
     8041 ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgccggggc aaacacagcc
     8101 tgggttccgt cgccaactgc cgctacgctc catgcgatgc actaccacca aaccaacgta
     8161 cagagcgtgc aagccaacat tgcccagacc gagttcaatg ctgaatttga accgctgctg
     8221 gacgatctgc tgactattcc ggtggctgaa aacgctaact ggtcggtgga agagatccaa
     8281 caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg ggtggagcag
     8341 gggattggtt gttcaaaagt gccggatatt cacaatgtgg cgctgatgga agaccgcgca
     8401 acgctgcgta tctccagcca gcatatcgcc aactggttac gtcacggtat tctgaccaaa
     8461 gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca aaacgctgac
     8521 gatccggctt atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt taaagctgcc
     8581 agcgatttaa tcttcctcgg cgtgaaacag ccaaacggct ataccgaacc gttattacac
     8641 gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaagccc cggcaagttt
     8701 tattgtcggg gctatgatag taactgcggt agtacattta tcccggaaat aaccggagcg
     8761 attatcaggg tttaacaata ttaaataccc gtagcgcatt actagcgggc ctaaaacaac
     8821 aatatggaca tcttaacgat actctgcgcc tgcgtgtgcg gaacagaaac ccttacccga
     8881 tatacttgtt atgccagagg tagcaattgc gttagcggcg tactgttgct gtaaagtata
     8941 acgtctatat tttacagttc agtcctcttt tgcttattac aaccttaagt atatcaagca
     9001 tataaagata ataagagact gaacaatatg gttacctgga cccaaatgta tatgccaatg
     9061 ggaggactgg ggctatccgc tctggtcgcc ctgatcccga taatattctt cttcgttgca
     9121 ctcgcggtat tacgtctgaa aggacatgtc gctggagcaa taacccttat attatctatc
     9181 ctgattgcaa tattcgcctt taaaatgccg attgatatgg cttttgctgc tgcgggctat
     9241 ggctttattt atggattatg gcctattgcg tggattattg tcgcggcggt gttcctctat
     9301 aaattaaccg ttgccagcgg tcagttcgat attatccgca gctcggttat ctccatcacc
     9361 gacgatcagc gtttgcaggt gttactgatt ggtttctcct ttggtgcgtt gctggaagga
     9421 gcggctggct ttggtgcgcc ggtggcgatt accggtgcgc tgctggtggg cctgggcttc
     9481 aaaccgttat acgcggcggg gctgtgtctg attgccaaca ctgcgccagt ggcgtttggt
     9541 gcgttgggcg tgccgattct ggtcgccggt caggtaacgg gaatcgatcc gttccacatt
     9601 ggcgcaatgg cgggacgtca gttaccgttc ctgtcggttc ttgtgccgtt ctggctggta
     9661 gcaatgatgg acggctggaa aggggtgaaa gagacgtggc cagcggcgct ggttgctggg
     9721 ggaagcttcg ctgtcactca gttctttacc tctaactata ttggtccgga actgccggat
     9781 attacttcgg cgctggtgag tatcgtctca ctcgctttat tccttaaagt ctggcggccg
     9841 aaaaataccg aaacggcaat cagcatgggg caatccgcag gtgcgatggt ggtaaataag
     9901 ccatcttctg gcggtcccgt gccttcagaa tatagtctgg ggcaaatcat tcgtgcgtgg
     9961 tcaccgtttt taatcttaac ggtgctggtc accatctgga ccatgaagcc gtttaaagcg
    10021 ttatttgctc cgggcggcgc gttttattca ctggtgatta atttccagat ccctcatttg
    10081 catcaacaag tgttgaaagc ggcacccatt gtcgcccaac caacgccaat ggatgcggtg
    10141 tttaaattcg accctctctc ggctggcggc accgctattt ttattgcggc gattatctct
    10201 atcttcatcc tcggcgtggg catcaagaaa gggattggcg tctttgccga aacgctaatt
    10261 agtctgaagt ggccgatatt gtcgattggc atggtgctgg cgttcgcctt cgtcaccaac
    10321 tattccggca tgtccaccac gctggcgctg gtactggcag gtacaggcgt gatgttcccg
    10381 tttttctcac cgttcctcgg ctggctgggg gtattcctta ccggctcgga cacttcttca
    10441 aacgccctgt ttggttcact gcaatcgact acggcgcagc aaatcaacgt ctctgacact
    10501 ctgctggtgg cagcaaatac cagcggcggc gtaactggca agatgatctc cccgcaatct
    10561 atcgccgtgg cctgcgccgc gacgggcatg gtgggccgag aatctgaact attccgctac
    10621 accgtgaagc acagtctgat ttttgccagc gttatcggcg ttatcacgtt gttgcaggcg
    10681 tatgtgttta ctggaatgtt ggtctcgtaa atcaataatg ccggatgatc gttaatatca
    10741 tccggcttca tttacattat tctgtcttat
//
LOCUS       DAZFWP020000163        10468 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFWP020000163 DAZFWP020000000
VERSION     DAZFWP020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.163"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..680
                     /gene="ssuE"
                     /locus_tag="ACHK0Z_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             105..680
                     /gene="ssuE"
                     /locus_tag="ACHK0Z_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU4504030.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            673..1632
                     /gene="ssuA"
                     /locus_tag="ACHK0Z_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             673..1632
                     /gene="ssuA"
                     /locus_tag="ACHK0Z_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU4504031.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADTRAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1629..2774
                     /gene="ssuD"
                     /locus_tag="ACHK0Z_RS20515"
                     /old_locus_tag="pgaptmp_004103"
     CDS             1629..2774
                     /gene="ssuD"
                     /locus_tag="ACHK0Z_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU4504032.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2785..3576
                     /gene="ssuC"
                     /locus_tag="ACHK0Z_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             2785..3576
                     /gene="ssuC"
                     /locus_tag="ACHK0Z_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU4504033.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3573..4340
                     /gene="ssuB"
                     /locus_tag="ACHK0Z_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     CDS             3573..4340
                     /gene="ssuB"
                     /locus_tag="ACHK0Z_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4504034.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4547..7159)
                     /gene="pepN"
                     /locus_tag="ACHK0Z_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     CDS             complement(4547..7159)
                     /gene="pepN"
                     /locus_tag="ACHK0Z_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU4504035.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7425..8627
                     /gene="pncB"
                     /locus_tag="ACHK0Z_RS20535"
                     /old_locus_tag="pgaptmp_004107"
     CDS             7425..8627
                     /gene="pncB"
                     /locus_tag="ACHK0Z_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU4504036.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIVHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8796..10196
                     /gene="asnS"
                     /locus_tag="ACHK0Z_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             8796..10196
                     /gene="asnS"
                     /locus_tag="ACHK0Z_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU4504037.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2369 a         2703 c         2809 g         2587 t
ORIGIN      
        1 agatacacaa aatttatatt tggcattttc ttgtctctcc gacagactga ccgaattgtt
       61 atcaatgtta acaaaaaaag aacaattggt tataaggaga gagtatgcgt gtcatcaccc
      121 tggcgggtag tcctcgcttt ccttctcgct ccagctcctt gctggaatat gcgcgggaaa
      181 aactaaatgg cctcgatgta gaggtttatc actggaatct gcaaaacttc gccccggaag
      241 atctgcttta tgctcgtttc gatagtccgg cactcaagac cttcaccgaa cagctgcaac
      301 aggctgatgg actgatagtc gctacacctg tgtataaagc cgcctattcc ggcgcgctga
      361 aaaccctgct cgacctgctg ccagagcgcg ctttgcaagg caaagtggtg ctaccgctgg
      421 cgacgggcgg taccgtggcc catctgctgg cggtcgatta tgcccttaaa ccagttttaa
      481 gcgcactgaa agctcaggag atcctgcacg gcgtgtttgc cgatgactca caagtaattg
      541 attaccatca cagaccccag ttcacgccaa atctgcaaac ccgtcttgat accgcgctag
      601 aaactttctg gcaggcattg caccgccgcg atgttcaggt tcctgacctt ctgtctctgc
      661 gaggtaatgc ccatgcgtaa catcattaaa ctggcgctgg tgggattgct tagcgtctct
      721 acgattgcgg ttgctgcaga atcctcgcct gaagcgttac gtataggcta tcagaaaggc
      781 agtattggta tggtactggc aaaaagccac cagttactgg aaaaacgcta tccgcaatca
      841 aaaatctcct gggtggagtt ccccgcgggt ccgcaaatgt tggaagcgtt aaacgttggc
      901 agtattgatc tcggcagtac cggggatatt ccgccaatct ttgcccaggc tgccggggct
      961 gatttggtat atgtgggcgt cgagccaccg aagcccaaag ctgaagtgat tctggtggca
     1021 gaaaacagcc cgatcaaaac cgtagccgat cttaaaggtc acaaagttgc ctttcagaaa
     1081 ggttccagtt cacacaacct tttactgcgt gcactgcgtc aggccggact taagtttacc
     1141 gatatccaac ccacttacct gacgcccgct gatacccgcg ccgcgttcca gcaaggtaac
     1201 gttgacgcct gggctatctg ggatccctac tactctgctg cattattaca gggcggcgtg
     1261 cgggtgctga aagacggcac cgatctcaat caaactggat cgttttatct ggcagctcgt
     1321 ccctatgcag aaaaaaacgg cgcttttatt cagggcgtac tcgcaacctt tagtgaggcc
     1381 gatgcgttaa cccgcagcca gcgcgagcaa agcatcgctt tactggcaaa aacgatgggc
     1441 ttaccggcac cggtgattgc ctcttactta gatcatcgcc ctcctaccac catcaaaccg
     1501 gttaacgccg aggttgccgc cttacagcag caaacggcag atctgtttta tgaaaatcgt
     1561 ctggtgccga aaaaagtcga tattcgccag cgcatctggc agcccactca actggaagga
     1621 aaacaattat gagtctgaat atgttctggt ttttaccgac ccatggtgac gggcattatc
     1681 ttggaacgga agaaggttca cgcccggttg atcacggcta tctgcaacaa attgcgcaag
     1741 cggcggatcg tctgggctat accggtgtgc tgatcccaac ggggcgatcg tgtgaagatg
     1801 cgtggctggt ggcggcgtcg atgatcccgg tgacgcagcg gctgaagttt cttgtcgccc
     1861 tgcgccccag cgtaacctca cctaccgttg ccgctcgcca ggccgccacg cttgaccgtc
     1921 tctcaaatgg tcgtgcgttg tttaatctgg tcacaggcag cgatccacaa gagctggcag
     1981 gcgacggcgt gttccttgat catagcgagc gctacgaagc ctcggcggaa tttacccagg
     2041 tctggcggcg cttattgctg ggggaaaccg tcaatttcaa cggtaaacat attcatgtgc
     2101 gcggagcaaa actgctcttc ccgccgattc aacagcctta tcctccgctt tactttggcg
     2161 gatcgtcaga cgtcgcccag gagctggcgg cagaacaagt tgatctctac ctcacctggg
     2221 gcgaaccgcc agaactagta aaagagaaga tcgaacaagt gcgggcgaaa gctgccgcgc
     2281 atggacgcaa aattcgtttc ggtattcgtc tgcatgtgat tgttcgtgaa actaacgacg
     2341 aagcgtggca ggccgccgag cggttaatct cgcatcttga tgatgaaact atcgccaaag
     2401 cacaggccgc attcgcccgg acggattccg taggacaaca gcgaatggcg gcgttacata
     2461 acggcaagcg cgacaatctg gagatcagcc ccaatttatg ggcgggcgtt ggcttagtgc
     2521 gcggcggtgc cggaacggcg ctggtgggcg atggtcctac ggtcgctgcg cgaatcaacg
     2581 aatacgccgc gcttggcatc gacagctttg tgctttcggg ctatccgcat ctggaagaag
     2641 cgtatcgggt cggcgagttg ctgttcccac atctggatgt cgccattccg gaaattcccc
     2701 agccgcagcc gctgaatccg caaggcgaag cggtggcgaa tgattttatc ccccgtaaag
     2761 tcgcgcaaag ctaaggaaga aataatggca acgccagtga agaagtggtt attgcgcgtt
     2821 gccccctggt ttttaccggt gggcatcgtg gcggtgtggc aactggcctc ctcagttggc
     2881 tggctgtcga cgcgtatttt gccctcaccg gaaggggtgg tgacggcgtt ctggacgctc
     2941 tccgccagcg gcgaactgtg gcagcatctg gcgatcagtt cctggcgggc gctgattggc
     3001 ttttcaattg gcggatcgct ggggttgatt ttgggactga ttagcgggct atctcgctgg
     3061 ggagaacggc tgctggatac ctcaattcag atgttgcgca acgtaccgca tctggcgctg
     3121 atcccgctgg tgattttgtg gtttggcatt gatgaatccg caaaaatctt tctggtggcg
     3181 ctcggcacgc tattccccat ttatatcaac acctggcatg gcatccgtaa tatcgatcgc
     3241 ggtctggtgg aaatggcgcg tagctatgga ttatccggca taccgctgtt tatccatgtg
     3301 atcctgcctg gtgccctgcc ctcaattatg gtcggcgtgc gttttgcgtt gggcctgatg
     3361 tggctgacgc tgattgttgc cgaaaccatt tctgccaatt caggcattgg ttatctggcg
     3421 atgaatgcgc gggagttttt gcaaacggac gtggtggtag tcgctattat tctttacgcc
     3481 ctgctcggca aacttgctga cgtcagcgcg cagctgttag agcgcctctg gttgcgctgg
     3541 aacccggctt atcatttgaa ggaggccacg gtatgaatac tgctcgtctg aaccagggca
     3601 caccattgtt gctcaatgca gtaagcaaac attacgcgga aaatatcgtc ctgaaccaac
     3661 tggatttaca tattccggca ggtcagtttg tggcggtggt gggccgcagc ggtggtggca
     3721 aaagtaccct gctgcgcctg ctggcaggtc tggaaacgcc aaccgcaggc gatgtgttag
     3781 cgggcaccac accgctggct gagattcagg aagacacgcg aatgatgttt caggatgcgc
     3841 gtctgctgcc gtggaaatcg gtgattgata acgttgggtt aggccttaaa ggccagtggc
     3901 gcgatgccgc gcgtcgggcg ttggctgcag taggactgga gaatcgcgcc ggggaatggc
     3961 ctgccgcact ttctggcggg cagaaacagc gtgtggcgct ggcaagagcg ttgattcatc
     4021 gaccgggttt attgttgctt gatgaaccgc tcggggcgct ggacgcctta acgcgactcg
     4081 agatgcagga tttgattgtg tcactttggc agcagcacgg ctttaccgta ctgttggtga
     4141 cgcatgatgt gagcgaagcg gtagcaatgg ctgaccgggt gttgttaatt gaagagggaa
     4201 aaattggtct ggatttgaca gtggatatcc cccgtccgcg tcgattaggg tcggtgcgat
     4261 tagcggagct ggaagcggaa gtattgcaac gggtaatgcg gcgtgggcat tctgaacaac
     4321 tgattcggcg tcatggttaa tatgccggat gtggcgcaca aaaccagcat tttcaacgga
     4381 ctagtaggcc tgataagaca cgtcaagcat cgcatcaggc agttgggcac ggctgccgga
     4441 tgcggcgtga acgccttatc cagcctacaa aaccagcaat ttcaacggag ttgtaggcct
     4501 gataagacgc gtcaagcgtc gcatcaggca ttttggctca aaagtatcaa gccagtgctt
     4561 tggtgatctt ctcgtacaga tcgccagaga gattttccag ccctttcaac tgttccagcg
     4621 ccgcgcgcat tttctcctga cgtttggcat cataacgttt caggcgaatc agcggttcaa
     4681 tcagacgtga agctacctgc gggttacggc tgttgaggtc ggtaagcatt tccaccagga
     4741 actggtaacc gctgccatct tcggcatgga acgctgccgg gttgctgccc gcaaacgcac
     4801 caatcaacga acgaatacgg ttcgggttgc tcatggtaaa tgagcgatgc tgcaacagac
     4861 cacgcaccgt ctccagcaca ttcgccgccg ggctggtggc ttgcaggata aaccatttat
     4921 ccatcaccag accgtcctga tgccacttat cgtcgtactc ctgcatcagc gcgtcacggc
     4981 aaggcagctg tgcagcaacc gccgcagaaa gcgctgccag cgcatcggtc atattgtttg
     5041 cttcatggta ttgcttgctc accagcacgt cagccagatg cgtttcacca aaagcaagga
     5101 agcgcaggca ggcattacgc agagtgcgtt ttgcaatatc ttcatgctca acacggtact
     5161 cgctctggta attcgcgttg taaatagcca gcagctcatc cgccagttca gtcgccagag
     5221 tacgagtgag tgcttcgcgt acttcggcaa tagcaatcgg gtcgatgata tcgaacagtt
     5281 cagccatttc attgacagaa ggcagcgtca ggatttctgc cgccagcgcc ggatcaatct
     5341 tctcatcgag cagcaccgcg cggaaagcgt cagccacatg taccggcaga gacagcggct
     5401 gcccttgctg atgacgggcg acgttcagct tgatgtaggt tgccagcaaa ctttgcgccg
     5461 catcccaacg ggagaaatca ttacgcgcat gacgcatcag gaaggtcagt tgctgatcgc
     5521 tccacttata ttccagtttc actggcgcag agaattcgca cagcagcgca ggcaccggct
     5581 ggaagtagac attatcaaaa acaaaggtct gttccgcctg ggtgacgttc agcacggaat
     5641 tcaccggatg accgcctttc tgcaacggga tcactttgcc ttcgttgtca tacaattcga
     5701 tggcaaatgg aatatgcagc ggctgttttt ctgcctgatc cggcgtggct ggcgtgcgct
     5761 ggctgatggt cagggtgtac tgctcggttt ccggattgta gtcgtctttg acggtcacaa
     5821 tcggcgtgcc ggactggctg taccaacggc ggaaatggga gagatcgaca ttcgacgcat
     5881 cttccatcgc ctgcacaaag tcgtcgcagg ttgccgcgct gccatcatgg cgctcgaaat
     5941 atagttgcat ccctttctgg aagttttctt cgcccagcag ggtgtggatc atgcgaatca
     6001 cttccgcgcc cttctcgtaa acggtcaggg tgtagaagtt gttcatctca atgaccatat
     6061 ccgggcggat cgggtgcgcc atcgggctgg cgtcttccgc aaactgcaat ccgcgcatgg
     6121 tgcgcacatt attgatacgg tttaccgcgc gggaaccaag gtcagagctg aattcctgat
     6181 cgcggaagac ggttaaacct tctttcaggc tgagctggaa ccagtcgcgg caggtcactc
     6241 ggttaccggt ccagttatgg aaatattcat ggccgataac gcgttcaata tcgagataat
     6301 ctttgtcggt ggcggtgtcg gtgcgggcca acacatattt ggagttaaag atattcagac
     6361 ccttattctc cattgcgccc atattgaaga aatccaccgc gacgatcata tagatgtcga
     6421 ggtcatactc caggccaaag cgttcttcat cccatttcat ggagtttttc agcgaggtca
     6481 tcgcccacgg cgcgcgatca aggttgccgc gatcgacgta cagctccagt gctacttcgc
     6541 gaccagaacg cgtggtaaag gtatcgcgca gtacatcaaa gtcgcctgcc accagcgcaa
     6601 acaggtagca cggtttcggg aacgggtcct gccactgtac ccaatggcgt ccgttttcca
     6661 gttcgccttg cgcaacgcgg ttaccattgg aaagcaggaa gggatatttg attttatcgg
     6721 caataatttt ggtggtaaaa cgcgccagca cgtccgggcg gtcgagataa tacgtaatat
     6781 ggcggaaacc ttcggcttca cactgggtgc aaagcgcatc gcctgactga taaagccctt
     6841 ccagcgccgt attcgccgcc gggcttattt cattaatgat cttaagcgta aaacgctccg
     6901 gcaaattact gatgaccagt gcgccctctt cttctttcca ggcggtccac ggctcatcat
     6961 taatatgaac agaaaccagt ttgaggtctt cgccgttgag acgaagcgga gcatctgatg
     7021 caccatgacg gacagcctgg ctgaccgcgg taacgaccgt cttttgcgcg tcgaggtcaa
     7081 aggtcaagtc aatatcagta atctggtaat ccggcgcacg ataatcgtga cggtatttgg
     7141 cttgtggctg ttgagtcata aataaccttt agcatctttt atagagtctg gtgttcagtc
     7201 tattcctgtt gcgtaaatcg cgctatgcag aatcttcatc ttttcaggta caaacgcctt
     7261 tattgctaca tttttataac atacaccgcg taatgccatc gaccagaaag gtggcatatg
     7321 gtgtgatcgg ggttcaataa attgcgaaac aaggtatact ccagcagttc ctgaagatgt
     7381 ttattgtact aaacgctcct gtacgaggac gctactgcgc acctatgaca caattcgctt
     7441 ctcctgttct gcactcgttg ctggatacag atgcttataa gttgcatatg cagcaagccg
     7501 tgtttcatca ctattacgat gtgcatgtcg cggcggagtt tcgttgccga ggtgacgatc
     7561 tgctgggtat ttatgccgat gctattcgtg aacagattca ggcgatgcag cacctgcgcc
     7621 tgcaggatga tgaatatcag tggctctctg ctctgccttt ctttaaggcc gactatctta
     7681 actggttacg cgagttccgc tttaacccgg aacaagtcac cgtatccaac gataatggca
     7741 agctggatat tcgtttaagc ggcccgtggc gtgaagtcat cctctgggaa gttcctttgc
     7801 tggcggttat cagtgaaatg gtacatcgct atcgctcacc gcaggccgac gttgcgcaag
     7861 ccctcgacac gctggaaagc aaattagtcg acttctcggc gttaaccgcc ggccttgata
     7921 tgtcgcgctt ccatcttatg gattttggca cccgtcgccg tttttctcgc gaagtacaag
     7981 aaaccatcgt taagcgtctg caacaggaat cctggttcgt gggcaccagc aactacgatc
     8041 tggcgcgtcg gctttccctc acgccgatgg gaacacaggc acacgaatgg ttccaggcac
     8101 atcagcaaat cagcccggat ctagccaaca gccagcgagc tgcacttgct gcctggctgg
     8161 aagaatatcc cgaccaactt ggcattgcat taaccgactg catcactatg gatgctttcc
     8221 tgcgtgattt cggtgtcgag ttcgccagtc gctatcaggg cctgcgtcat gactctggcg
     8281 acccggttga atggggtgaa aaagccattg tacattatga aaagctggga attgatccac
     8341 agagtaaaac gctggttttc tctgacaatc tggatttacg caaagcggtt gagctatacc
     8401 gccacttctc ttcccgcgtg caattaagtt ttggtattgg gactcgcctg acctgcgata
     8461 tcccccaggt aaaacccctg aatattgtga ttaagttggt tgagtgtaac ggtaaaccgg
     8521 tggcgaaact ttctgacagc cctggcaaaa ctatctgcca cgataaagcg tttgttcggg
     8581 cgctgcgcaa agcgttcgac cttccgcata ttaaaaaagc cagttaatat catcagggag
     8641 ctaatcggct cccttttttt acctttaatt ccgaaatctt tcgctgcatt tgcgaattct
     8701 gcttgtctga ttgcagatgc caggtaacat aggtatcccc ccatttacgg gtggacaagt
     8761 gtttattttt tccgactatt aacagagaga atattatgag cgttgtgcct gtagccgacg
     8821 tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc
     8881 gccgagattc aaaagctggc atctccttcc tcgccgttta tgacggttcc tgctttgatc
     8941 ctgtacaggc tgtcatcaat aattctctgc ccaattacaa tgaagacgtc ctgcgtctga
     9001 ccaccggctg ctcggtcatt gtgacgggta aagtcgtggc gtcgccgggc caggggcaac
     9061 aatttgaaat tcaggccagc aaagttgaag ttgctggttg ggttgaagat ccagacactt
     9121 acccgatggc ggcaaaacgc cacagcattg agtatctgcg tgaagtcgct cacctgcgtc
     9181 cgcgcacaaa cctgattggt gccgtcgcgc gcgttcgcca tacgctggcg caggcgctgc
     9241 atcgcttctt taacgagcag ggattcttct gggtttcaac gccactgatt accgcatctg
     9301 ataccgaagg tgcaggcgaa atgttccgcg tttctacgct ggatctggaa aacctgccgc
     9361 gtaacgatca gggcaaagtg gatttcgaca aagacttctt tggtaaagag tctttcctga
     9421 ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc attatccaaa atttatacct
     9481 tcggcccgac tttccgtgct gaaaactcca acaccagccg tcacctggcg gaattctgga
     9541 tgctggagcc ggaagtggcg tttgctaacc tgaacgatat cgcgggtctg gctgaagcca
     9601 tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc agacgacatg aaattcttcg
     9661 ctgaacgcgt agataaagat gccgtttcac gtctggaacg cttcattgag gccgatttcg
     9721 cgcaggtgga ttacaccgac gcagtgacca ttctcgaaaa ctgcggcagg aagtttgaaa
     9781 acccggttta ctggggcgtc gatctctctt ctgagcatga gcgttatctg gcggaagaac
     9841 actttaaagc accggtagtg gttaaaaact atccgaaaga tattaaagcg ttctatatgc
     9901 gcctcaacga agacggtaaa accgttgcgg ctatggacgt tctggctccg ggcatcggtg
     9961 agatcattgg tggctcccag cgtgaagaac gtctggacgt tctggacgag cgtatgctgg
    10021 aaatgggcct gaataaagaa gattactggt ggtatcgcga tctgcgtcgc tacggtactg
    10081 ttccgcattc aggtttcggt cttggttttg aacgcctgat tgcttacgta actggcgtgc
    10141 aaaacgtgcg tgatgtgatt ccgttcccac gtactccgcg caacgccagc ttctgatttt
    10201 cttcctcaaa gagccagcgc ctgctggctc ttttttatcc tttctgtcaa gttatctgtt
    10261 tgttaagtca agcaatctat ttgcaacccc gccataacga tcatcctgtt acggaatatt
    10321 acattgcaac atttacgcac aaaaaataac ccgcattctt attgcggatt agttttttct
    10381 tagctgatag cacaattttc atactatttt ttggcattct ggatgtctga aagaggattt
    10441 tgtgccaggt cgataaagtt tccatcag
//
LOCUS       DAZFWP020000164        10456 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFWP020000164 DAZFWP020000000
VERSION     DAZFWP020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.164"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(201..2234)
                     /gene="betT"
                     /locus_tag="ACHK0Z_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             complement(201..2234)
                     /gene="betT"
                     /locus_tag="ACHK0Z_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU4504038.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2363..2950
                     /gene="betI"
                     /locus_tag="ACHK0Z_RS20550"
                     /old_locus_tag="pgaptmp_004110"
     CDS             2363..2950
                     /gene="betI"
                     /locus_tag="ACHK0Z_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU4504039.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2964..4436
                     /gene="betB"
                     /locus_tag="ACHK0Z_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             2964..4436
                     /gene="betB"
                     /locus_tag="ACHK0Z_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU4504040.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4450..6138
                     /gene="betA"
                     /locus_tag="ACHK0Z_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     CDS             4450..6138
                     /gene="betA"
                     /locus_tag="ACHK0Z_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU4504041.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKKMSRDVN"
     gene            6335..7003
                     /locus_tag="ACHK0Z_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /pseudo
     CDS             6335..7003
                     /locus_tag="ACHK0Z_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7246..7941)
                     /locus_tag="ACHK0Z_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             complement(7246..7941)
                     /locus_tag="ACHK0Z_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4504042.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7934..9361)
                     /locus_tag="ACHK0Z_RS20575"
                     /old_locus_tag="pgaptmp_004115"
     CDS             complement(7934..9361)
                     /locus_tag="ACHK0Z_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4504043.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEANGESFRSWFKKHLAQEKKNG"
     gene            complement(9372..10091)
                     /locus_tag="ACHK0Z_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             complement(9372..10091)
                     /locus_tag="ACHK0Z_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4504044.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2660 a         2812 c         2577 g         2407 t
ORIGIN      
        1 tatataacac tataggactg aatccattca gctaatacaa aataaaatcg ttaattaata
       61 aaatataaaa tcatgaagtg atacaagagg aattgcagga tatgacatga caatcatata
      121 taatatatgc ataatttttt acagcgagtc aatgattccc gcgccatatg cagacatggc
      181 gcggttttat gcaaaaacaa tcacgcgtcc gggaacatca cgctatggcc cggcgcttca
      241 cgatggagat gaataaagtt aaggtgccgc tcgtactggt caagaatatc ggtgatcacc
      301 tgctctttgc tgtagtccat caggtcgttg ccctggctgc cttctaacag gaaggtttcc
      361 agccggtagt aggtcgattt accgctacgt gcgcggtagg taaagcccgg caccgaatat
      421 tgctgcggcc aaatctgata gacaaagttt tgttcttcgc ccatatgcac caacaaatcc
      481 agatgcccca actgttgtcc ctcttccggc ggcaggcttt ttagctccac gtatgcgccg
      541 cgcaaccgca gctcctgcgc cacttcttcc attgccgggt aacagaccgt ctccatcatc
      601 tgtttagtgt aacgcgtgcc cggataattc atcaggcgcg agagacgttt tttccagctc
      661 aggcgatcct gaagccccag cggtcgcggt gcggtatcgc ggttggcact ttcacggcgg
      721 taatcttcta ccttcagaga tttatacaac cccgccatca cgaagaagat cacaaagctg
      781 aacggcagcc ccataatcac cgtggtgttt tgcagcgcgg atatcccgtt ggtcatcagc
      841 atgccgagcg tcagcaggcc aatcgccacc gaccagaaga cgcgcagcca gccgggggcg
      901 tcgctgttga tatctttaag ctgcgaggtg aaattcccca gcaccagcgc accggagtcc
      961 gccgaggtca cataaaacag caggccagta atggtggcga cggaggcgct aaaggtaaac
     1021 gccggatact gcgccagcag gctgtaaaag ccgcgctccg gatggaccat cgcttcctcg
     1081 gcaaatgccg cgccgccgtg gatgatttca tacagcgcgc tattgccgaa caccgagagc
     1141 cataacagcg tgaaggtaaa cggaataatc aacgtgccca gcacgaactg gcgaatggta
     1201 cgcccacgcg agatacgcgc caggaacaag ccgacaaacg gcgaccatgc cacccaccat
     1261 gcccagaaga agagcgtcca gttattcatc cactcaactg gacggtcgaa ggcaaaactg
     1321 ttgagcgtca tgcccataaa gcgattcaca tagtcgccaa cattcagcac cagcgcatta
     1381 agcaggaacg aggtgtcgcc cataaacaat acgaacagga tcaatcccag cgccagcgca
     1441 acgttaagtt ccgataacac acgaatcccc ttatcgacac cggaggtgac agagatcgtg
     1501 gcgattatca ccgacaaggc gatcagtgcc gcttttgccg ccatcgaatc gggaatatca
     1561 aacagtacgc tcaagccata gttaagctgc accacaccga taccgagcgt agtggcaata
     1621 ccgaagatgg tgccgatcac cgctgcaata tccactgagt gccctatcgg cccgttaatc
     1681 cgtttaccga agatcgggta cagcgccgag cggatggtga gcggcaaatt ataacgatag
     1741 ctaaagtatc cgagcgccat gcccatcagc gcatacatcg accagccggt taagccgtag
     1801 tgaaacagcg tccagaccat cgcctgacgc gcggcctcaa tcgtctgtcc cgcgccttcc
     1861 ggcggctgca tatactgcgt taccggttcg gctacggaga agaacatcag gtcgataccg
     1921 atcccggcag caaacagcat cgccgcccaa ctcagcaggc tgaattccgg tttggattgt
     1981 tctggcccga gcttcaccga accaaaacgc gaacaagcga tacagaccac aaagacaata
     2041 tagagcgttg ccgccagtag atagtaccaa ccgaaggttt tagaaaccca gtccagcgtg
     2101 cggccaatcc acagggccga gaagtcgcga aacaggatcg ttgtcaggga aaacaacaaa
     2161 atcagtccgg cggaggtgta aaacaccacc ggattgattt tgtccttttc cctgctgtgt
     2221 gaaaggtctg tcatccagta tccccactgt tattgttacc atttaaaatc aaattcgtaa
     2281 caattaagac acattttata ttgaacgtcc aatcaataac cgctttaata gataaacaac
     2341 gctgatgaat ggagtggcga aaatgcccaa attggggatg cagtcgatcc ggcgcagaca
     2401 actgatcgac gccacactgg aagcaataaa tgaagtgggc atgcacgatg caacgatcgc
     2461 gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc agccactatt tcagggacaa
     2521 aaatggtctg ctggaagcaa ccatgcgcga catcaccagt cagttacgtg acgcggtttt
     2581 gaatcgatta catgcacttc cgcagggcag tgcagagcag cgattacagg cgattgttgc
     2641 cggaaacttc gatgaaactc aggtgagcag tgcggcgatg aaagcctggc tggcgttctg
     2701 ggccagcagt atgcatcagc cgatgctcta tcgtttacag caggtcagca gccgccgctt
     2761 gctgtcgaat ctggtgagcg agtttcgccg cgaattgccg cgcgaacagg cacaggaagc
     2821 gggctacggc cttgccgcgc tgattgacgg attatggctg cgcgcggcac tgagcggcaa
     2881 accgctggat aaacctctcg cacactcgct gacccgtcac tttattactc agcatttacc
     2941 caccgattaa ccgaggagac gtgatgtccc gaatggcaga acagcagctt tatatacatg
     3001 gtggttatac ctccgccacc agcggtcgca ccttcgagac cattaacccg gccaacggta
     3061 acgtgctggc gaccgtgcag gccgccgggc gcgaggatgt cgatcgcgcc gtgaagagtg
     3121 cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat ggaacgctcg cgtattctgc
     3181 gtcgggccgt tgatattctg cgtgaacgca atgacgaact cgcaaaactt gaaaccctcg
     3241 acaccggaaa agcatattcg gaaacctcaa ccgtcgatat cgttaccggt gcggacgtgc
     3301 tggagtacta cgccgggctg atcccggctc tggaaggcag ccagatcccg ttgcgtgaga
     3361 cgttatttgt ttatacccgc cgcgaacctc tgggtgtggt ggcggggatt ggcgcatgga
     3421 actacccgat tcagattgcc ctgtggaaat ccgccccggc gctggcggca ggcaacgcaa
     3481 tgattttcaa accgagcgaa gtcaccccgc ttaccgcgtt aaagctggct gaaatttaca
     3541 gcgaagcggg cctgccggac ggcgtattta acgtgttgcc gggggtgggc gcggagaccg
     3601 ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc atttaccggc ggtgtcgcca
     3661 gcggcaaaaa agtgatggct aactcggcgg cctcttccct gaaagaagtg accatggaac
     3721 tgggcggtaa atcaccgctg atcgttttcg atgatgcgga tctcgatctc gccgccgata
     3781 tcgccatgat ggcaaacttc ttcagctccg gtcaggtgtg taccaatggc acccgcgtct
     3841 tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat tctggcgcgc gttgagcgca
     3901 ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcgg cccgctggtc agcttcccgc
     3961 atcgcgataa cgtgctgcgc tatatcgcca aaggcaaaga ggaaggcgcg cgcgtactgt
     4021 gcggcggcga tgtactgaaa ggcgatggct tcgataacgg cgcatgggtt gcaccgaccg
     4081 tgttcaccga ttgccgcgac gaaatgacca tcgtgcgcga agagatcttc ggtccggtga
     4141 tgtcgcttct cacttatgaa tctgaggacg aagtgattcg ccgcgccaat gataccgact
     4201 acggcctggc ggcgggcatc gtgacggcgg acctgaaccg cgcgcatcgc gtcattcatc
     4261 agctggaagc gggtatttgc tggatcaaca cctggggcga atccccggca gagatgcccg
     4321 ttggcggcta caaacactcc ggcattggtc gcgagaacgg cgtgatgacg ctccagagtt
     4381 acacccaggt gaagtccatc caggttgaga tggctaaatt ccagtccata ttctaaccag
     4441 gaggtttatt tgcaatttga ctacatcatt attggtgccg gctcagccgg caacgttctc
     4501 gctacccgtc tgactgaaga tccgaatacc tccgtgctgc tgcttgaagc gggcggcccg
     4561 gactatcgct ttgacttccg cacccagatg cccgcagcgc tggcgttccc actacagggc
     4621 aaacgctaca actgggccta tgaaacagag cctgaaccgt ttatgaataa ccgccgcatg
     4681 gagtgcggac gcggtaaagg cctgggtgga tcgtcgctga tcaacggcat gtgctacatc
     4741 cgtggcaatg cgctggatct cgataactgg gcgcaagaac ccggtctgga gaactggagt
     4801 tatctcgact gcctgcccta ctaccgcaag gccgagactc gcgatgtggg cgagaacgac
     4861 taccacggtg gcgatggccc ggtgagcgtc accacctcca aacccggcgt caatccgctg
     4921 tttgaagcga tgattgaagc gggcgtgcag gcaggctacc cgcgcacgga cgatctcaac
     4981 ggctatcagc aggaaggttt tggcccgatg gatcgcaccg tcacgccgca gggccgtcgc
     5041 gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc gccctaacct gaccattcgt
     5101 actcacgcta tgaccgatca catcattttt gacggcaaac gcgcggtggg cgtcgaatgg
     5161 ctggaaggcg acagcaccat cccaacccgc gcaacggcca acaaagaagt gctgttatgt
     5221 gcaggcgcga ttgcctcacc gcagatcctg caacgctccg gcgtcggcaa cgctgaactg
     5281 ctggcggagt ttgatattcc gctggtgcat gaattacccg gcgtcggcga aaatcttcag
     5341 gatcatctgg agatgtatct gcaatatgag tgcaaagaac cggtttccct ctaccctgcc
     5401 ctgcagtggt ggaaccaacc gaaaatcggt gcggagtggc tgtttggcgg cactggcgtt
     5461 ggtgccagca accactttga agcaggtgga tttattcgca gccgtgagga atttgcgtgg
     5521 ccgaatattc agtaccattt cctgccagta gcgattaact ataacggctc gaatgcagtg
     5581 aaagagcacg gtttccagtg ccacgtcggc tcaatgcgct cgccaagccg tgggcatgtg
     5641 cggattaaat cccgcgaccc gcaccagcat ccggcgattc tgtttaacta catgtcgcac
     5701 gagcaggact ggcaggagtt ccgcgacgca attcgcatca cccgcgagat catgcatcaa
     5761 cccgcgctgg atcagtatcg tggccgcgaa atcagccccg gcacggaatg tcagacggat
     5821 gaacagctcg atgagttcgt gcgtaaccac gccgaaaccg ccttccatcc gtgcggtacc
     5881 tgcaaaatgg gttacgacga gatgtccgtg gttgacggcg aaggccgcgt acacgggtta
     5941 gaaggcctgc gtgtggtgga tgcgtcgatt atgccgcaga ttatcaccgg gaatttgaac
     6001 gccacgacaa ttatgattgg cgagaaaata gcggatatga ttcgtggaca ggaagcgctg
     6061 ccgaggagca cggcgggata ttttgtggca aatgggatgc cggtgagagc gaaaaaaatg
     6121 agtcgtgatg tgaactgact caagaaccga tcagattgag agttaccgtt ccaggcatgg
     6181 ggacagaatc gttatataaa acataggggt aactctcatg cttcatatcc agtattcatg
     6241 atgctggctt tttgctgata tttaacccga acattcctga tacagactat taatacaacc
     6301 ttatctgcga cagattttat tttctggata tattatgcgt gaacaaatca aacaggatat
     6361 cgatctgatt gagattttat tttatctgaa gaaaaaggtt cgtgttatcc tttttattat
     6421 agctatctgt atggcaatga tgcttttatt tctgtatatc aataaagata atataaaagt
     6481 gagttacagc ctaaaaataa accagacaac accagacata cttgttaact gtgatagcaa
     6541 taataacttt gcctgtcaga ccgcaatgac taaagatgtt atacagcgaa ttactacatt
     6601 ttttcacacc agcccagatg tcaagaacag agaaataaag ctggaatggt catgagataa
     6661 gagagattta ccaactgctg aatcggaaat atctcgcgtg caggcgtcta ttatcaaatg
     6721 gtatgcgtca gaataccata atggcaggca agttctcgat gagatacaaa taccttcagc
     6781 aattaacagt gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc
     6841 gaacggtgat ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc
     6901 catttgcctg gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt
     6961 caaaaaaatg gtagatgaat accaacaaaa ctctgggcag taataaacaa cattctgata
     7021 gagagtgcct gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat
     7081 ggatgcctgt ctttttcact gtaaatatcc tggcattact aatgccagga tatttacaga
     7141 agagcagcat aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa
     7201 cctcgtatgc cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat
     7261 aatcagatac accgctttca ccgggccatg aaccccgacg actttgataa gctcaatatc
     7321 cgccgttgaa ctagggccgc taatgatgtt aatgcaggac ggcattcgtt caccggcctg
     7381 cgctttctga tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg
     7441 caggataaaa agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga
     7501 aaaaagaacc acgcctcccg attcggttaa accatattca gcatacacga cacccacttt
     7561 agcctgctct gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt
     7621 gcattcctgc tgcaaacgtt cgctaatccc caattcctct agcctcgtgt caccgctaat
     7681 cacgaccgac tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc
     7741 ctcgctggtc agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc
     7801 acagcgctgc tgttggttaa gtgcggtaag ccgctcgtta gcatagttgt taagcggcgc
     7861 atcttctgct tgcggttcaa gtcgcaacgg gcgacccagt gcctgagcaa cgttattcaa
     7921 aaattcgctc cgattatcca ttctttttct cctgcgccag atgtttctta aaccaactac
     7981 ggaaactctc tccgttagct tcaggaagat cgcgtgcttc catccagtca ctaatcgcgc
     8041 caaatttgag tggtgttttg ccgccattga taaaccagct tgccgcatga gcaccggcca
     8101 tcatcccgac tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc
     8161 gttgctctgc ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca
     8221 gttttgacag cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg
     8281 cgtagggtaa atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa
     8341 ttggccctgg ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag
     8401 tattcataca agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca
     8461 cctcagaacg cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc
     8521 ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac
     8581 tgcgcgccag catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca
     8641 ttcccatcac tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat
     8701 tggtcaccag gcataccgaa cctgtctctg ccaccgcgaa attacagccg gtaataccta
     8761 tttcagcact gaggaaatct tcgcggattt tttgccggat gaataaggtc atcgcttcag
     8821 gcgtttccgg cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat
     8881 ctttatgaat tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat
     8941 attcacccag atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac
     9001 caatctcttc ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg
     9061 caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt tgcaaaccag acgtgaccgc
     9121 cgttttgcgt cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat
     9181 gatcacgtat ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca
     9241 ttttttgccg atttgcccca atacgctgct gcgcgtttgc caccgctttg cgcatgatcg
     9301 gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca
     9361 tctttatatc ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc
     9421 ccttcccgtt gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa
     9481 tactcagggc ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt
     9541 tcggccattt tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag
     9601 gtcaacagct ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc
     9661 agcttacggg ccaggctaca agatgggtga tatactgctc tcccttgcaa actggcacct
     9721 acatcgacta cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca
     9781 accttttcgg cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt
     9841 acggcatagg tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg
     9901 gcgatcagat ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc
     9961 tgaccgcagc atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc
    10021 agcacggagt ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa
    10081 ttgacattca caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc
    10141 caccggggga tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc
    10201 acccctcatt tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat
    10261 tttatttctt tcggcatcgc tcacatttta tggggaaatc gacgttcatt tttggcataa
    10321 gaaatccatt taaaaaggct acatccctta tacctattca gagctactta ttcaggggtt
    10381 attgagatat acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa
    10441 tagtcggggt tttatt
//
LOCUS       DAZFWP020000165        10285 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFWP020000165 DAZFWP020000000
VERSION     DAZFWP020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.165"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..154)
                     /locus_tag="ACHK0Z_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             complement(<1..154)
                     /locus_tag="ACHK0Z_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4504045.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPG"
     gene            complement(241..309)
                     /gene="ykiE"
                     /locus_tag="ACHK0Z_RS20590"
                     /old_locus_tag="pgaptmp_004118"
     CDS             complement(241..309)
                     /gene="ykiE"
                     /locus_tag="ACHK0Z_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HGU4504046.1"
                     /translation="MGWSLCFWHVSVKMTHDVVCYY"
     gene            446..967
                     /gene="fecI"
                     /locus_tag="ACHK0Z_RS20595"
                     /old_locus_tag="pgaptmp_004119"
     CDS             446..967
                     /gene="fecI"
                     /locus_tag="ACHK0Z_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HGU4504047.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            964..1917
                     /gene="fecR"
                     /locus_tag="ACHK0Z_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             964..1917
                     /gene="fecR"
                     /locus_tag="ACHK0Z_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HGU4504048.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGASGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            2004..4328
                     /gene="fecA"
                     /locus_tag="ACHK0Z_RS20605"
                     /old_locus_tag="pgaptmp_004121"
     CDS             2004..4328
                     /gene="fecA"
                     /locus_tag="ACHK0Z_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HGU4504049.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGITLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPALAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRSYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYGSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4373..5275
                     /gene="fecB"
                     /locus_tag="ACHK0Z_RS20610"
                     /old_locus_tag="pgaptmp_004122"
     CDS             4373..5275
                     /gene="fecB"
                     /locus_tag="ACHK0Z_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HGU4504050.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5272..6270
                     /gene="fecC"
                     /locus_tag="ACHK0Z_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             5272..6270
                     /gene="fecC"
                     /locus_tag="ACHK0Z_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HGU4504051.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6267..7223
                     /gene="fecD"
                     /locus_tag="ACHK0Z_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             6267..7223
                     /gene="fecD"
                     /locus_tag="ACHK0Z_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HGU4504052.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIYPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7224..7991
                     /gene="fecE"
                     /locus_tag="ACHK0Z_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             7224..7991
                     /gene="fecE"
                     /locus_tag="ACHK0Z_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HGU4504053.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGHLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8527..8931)
                     /locus_tag="ACHK0Z_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             complement(8527..8931)
                     /locus_tag="ACHK0Z_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HGU4504054.1"
                     /translation="MMRGILPPVSVCRMAIPWWITRIHGVTTSAPLITGAGGGVGVSI
                     GAGGNGAGISVFASVNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGN
                     RIIADVGHDLLISSQQDTSKYDSKQTSVAAGD"
     gene            complement(8813..9841)
                     /locus_tag="ACHK0Z_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /pseudo
     CDS             complement(8813..9841)
                     /locus_tag="ACHK0Z_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
BASE COUNT         2303 a         2929 c         2793 g         2260 t
ORIGIN      
        1 taccgggctg agaggcggtg taagtgaact gtagttgcca tgttttacgg caatgagagc
       61 agagatagcg ctgatgtccg gcagtgcttt tgccgttacg caccacgcct tcagtagcgg
      121 agcaggaagg acatctgatg gaaatggaag ccacgcaagc accttaaaat caccatcata
      181 cactaaatca gtaagttggc agcattaccg aaatccccat cctttagggc agtgagaatg
      241 tcaataatag cagaccacat catgagtcat tttgaccgac acatgccaga agcagaggct
      301 ccatcccatt ttatacctac cctataacac ttagaaaaac aacatgttaa aaatgtctat
      361 tggaaacaat tttatttcca attgtaatga taaccattct catattaata tggctacgtg
      421 ataattaact tttgatgcac tccgcatgtc tgaccgcgcc actaccacag cttccttaac
      481 gttcgagtcg ctttatggca cacatcacgg ctggttgaaa agctggctga cgcgcaaact
      541 ccagtctgct tttgatgcag atgacattgc ccaggacact tttttgcggg taatggtcag
      601 cgaaacgctc tcgacgatcc gcgatcctcg ctccttcctg tgcactatcg ccaaacgcgt
      661 gatggtggac ctgtttcgcc gaaacgcgct ggaaaaagcg tatctggaga tgctggcgct
      721 tatgccggag gggggagcgc cttcacctga ggaacgcgaa agccaactcg agaccctaca
      781 actcctcgac agcatgctgg acgggctaaa cggcaaaaca cgtgaagcgt ttctgctttc
      841 gcaactggat ggtctgacat acagcgagat tgcgcacaaa ctcggtgttt ccatcagctc
      901 cgtgaaaaaa tacgtggcga aagccgtcga gcactgcctg ctgttccgtc tggagtatgg
      961 gttatgaatc ctttgttaac cgattcccgc cgtcaggcgc tgcgttcagc ttcccactgg
     1021 tatgccgtgc taagcggcga gcgcgtcagc ccacaacagg aagcgcgctg gcaacagtgg
     1081 tatgaacagg atcaggataa ccagtgggcc tggcagcagg ttgaaaacct gcgcaaccag
     1141 cttggcggtg tgcctggcga cgttgccagc cgggcgttgc acgatacccg cctcacccgc
     1201 cgtcacgtga tgaaaggatt actgctgttg ctcggcgcta gcggaggctg gcagctctgg
     1261 cagtcggaaa ccggcgaagg tctgcgggca gattaccgca ccgccaaagg cacagtcagc
     1321 cgtcagcaac tggaagatgg ctccctgctc acgctgaata cccaaagcgc ggcggatgtg
     1381 cgttttgatg cgcatcagcg caccgtccgg ctctggtacg gtgaaatcgc cattactacc
     1441 gcgaaagatg ccctgcaacg ccccttccgt gtcctgaccc gtcagggcca gctcactgct
     1501 ttagggacag aatttaccgt ccgccagcag gataatttca cgcagcttga cgtgcagcag
     1561 cacgccgtgg aagtgcttct cgccagtgcc cccgcgcaaa aacgcatcgt gaacgctggt
     1621 gaaagcctgc agttcagcgc ctctgagttt ggcgcagtga aaccgctgga tgacgagagt
     1681 acaagctgga cgaaggacat cctgagcttc agcgataaac cgctgggtga ggtgatagcc
     1741 acgctaaccc gttaccgcaa cggcgtgctg cgctgcgatc ccgccgttgc cgggctgcgc
     1801 ctgagcggga cgttcccgct gaaaaatacc gatgcgatcc tgaacgttat cgcgcaaacg
     1861 cttcccgtta aaattcagtc tattacgcgg tactggataa acatttcacc actgtaagga
     1921 aaataattct tatttcgatt gtccttttta cccttctcgt tcgactcata gctgaacaca
     1981 acaaaaatga tgatggggaa ggtatgacgc cgttacgcgt ttttcgtaaa acaacacctt
     2041 tggttaacgc cattcgcctg agcctgctgc cgctggccgg tctctcgttt tccgcttttg
     2101 ctgcacaggt taatatcgca ccgggatcgc tcgataaagc gctcaatcag tatgccgcac
     2161 acagcggaat taccctctcg gttgacgcca gcctgacgcg cggcaagcag agcaacggcc
     2221 tgcacggcga ttacgacgtc gagagcggcc tgcaacaact gctggacggc agcggactgc
     2281 aggtaaaacc gctgggaaat aacagctgga cgctggagcc cgcgctcgca ccaaaagaag
     2341 atgccctgac cgtggtcggc gactggctgg gtgatgcgcg tgaaaacgac gtatttgaac
     2401 atgctggcgc gcgtgacgtg atccgccgtg aggatttcgc caaaaccggc gcaaccacca
     2461 tgcgtgaggt acttaaccgc atccctggcg tcagcgcgcc ggaaaacaac ggcaccggca
     2521 gccacgacct ggcgatgaac tttggcatcc ggggcctgaa cccgcgcctc gccagccgct
     2581 cgaccgtcct gatggacggc atccccgtcc ccttcgcccc ttacggtcag ccgcagcttt
     2641 cactggctcc cgtttcgctc ggcaacatgg atgccattga cgtggtacgc ggtggtggtg
     2701 cggtgcgtta cggaccgcag agcgtgggcg gcgtggtgaa ctttgttacc cgtgccattc
     2761 cgcaggactt tggtatcgag gcgggcgtgg aaggtcagct cagcccaacc tcttcacaaa
     2821 acaacccgaa agagacgcac aacctgatgg tgggcggcac agcggacaac ggttttggca
     2881 ccgcgctgct ctactccggc acgcgcggca gtgactggcg cgagcacagc gccacccgca
     2941 tcgacgacct gatgctgaaa agcaaatatg cgccggatga ggtgcacacc ttcaacagcc
     3001 tgctgcaata ttacgacggt gaagccgaca tgcccggtgg cctgtctcgc gcggattacg
     3061 acgccgatcg ctggcaatcc acccgctcgt atgaccgctt ctggggtcgt cgcaagctgg
     3121 cgagcctggg ctaccagttc cagccagaca gccagcataa attcaacatt caggggttct
     3181 acacccaaac cctgcgcagc ggctacctgg agcaaggcaa acgcatcacc ctctcgccgc
     3241 gtaactactg ggtgcgcggt attgagccac gctacagcca gatctttatg atcggccctt
     3301 ccgcgcacga agtgggcgtg ggctatcgct atgtgaatga atcaacgcat gaaatgcgtt
     3361 actacaccgc caccagcagc gggcagttgc cgtccggctc aagcccttac gaccgcgata
     3421 cgcgttccgg caccgaggcg cacgcctggt atctggatga caaaatcgac atcggcaact
     3481 ggaccatcac gccgggtatg cgtttcgaac atatcgagtc ataccagaac aacgccatca
     3541 caggcacgca cgaagaagtg agctataacg caccgcttcc ggcgttgaac gtgctctatc
     3601 acctgactga cagctggaat ctttatgcaa acactgaagg ctcgttcggc accgtacagt
     3661 acagccagat tggcaaggct gtgcaaagcg gcaatgtgga accggaaaaa gcgcgaacct
     3721 gggaactcgg tacccgctac gacgacggcg cgctgacggc ggaaatgggg ctgttcctga
     3781 ttaactttaa caatcagtac ggctccaacc agaccaacga caccgtcact gcacgtggca
     3841 aaacgcgcca taccgggctg gaaacgcagg cacgttacga tctgggtacg ctaacgccaa
     3901 cgcttgataa cgtttccatc tacgccagct atgcgtatgt gaacgcggaa atccgcgaga
     3961 aaggcgacac ctacggcaat ctggtaccat tctccccgaa acataaaggc acgctgggcg
     4021 tggactacaa gccaggaaac tggacgttca atctgaacag cgatttccag tccagccagt
     4081 ttgcggataa cgccaatacg gtgaaagaga gcgccgacgg cagtaccggc cgcattcccg
     4141 gcttcatgct ctggggcgca cgcgtggcgt atgactttgg cccgcagatg gcagatctga
     4201 acctggcatt cggtgtgaaa aacatcttcg accaggacta cttcatccgc tcttatgacg
     4261 acaacaacaa aggcatctat gcaggccagc cgcgcacgct gtatatgcag gggtcgttga
     4321 agttctgatg tgttaacgcc cggcttgccg ggcttttagc tggaatgtga ttatgttggc
     4381 atttatccgt tttctttttg caggcctgct gctggtgatc agccacgcct ttgccgccac
     4441 ggttcaggac gaacacggca cgtttacact cgaaaaaacg ccacaacgga ttgtggtgct
     4501 ggaactctcg ttcgccgatg cgctggccgc cgtggacgtc agcccgatcg gtattgccga
     4561 cgataacgat gcaaaacgca tcctgcccga agtgcgtgcg cacctgaaac cgtggcagtc
     4621 cgtcggaacg cgcgcgcagc cgagcctgga agccattgcc gctctgaaac cagacctgat
     4681 cattgccgac agcagtcgcc atgcgggggt ttacatcgcc ttgcagcaaa tcgcgccggt
     4741 actgctgctt aagtcccgca acgaaaccta cgctgaaaat ttgcaatctg cggctatcat
     4801 cggcgaaatg gtgggtaaaa agcgagagat gcaggcacgt ctggaacaac ataaagagag
     4861 gatggcgcag tgggccagcc agcttcccaa agggacacgc gtggcctttg gcacatcacg
     4921 cgaacagcaa ttcaacctgc atactcagga gacctggacc ggcagcgtgc tggcctctct
     4981 ggggctgaac gttcccgctg cgatggcggg tgcgtccatg ccgtccatcg gcctggagca
     5041 actgctggcg gtcaatcctg cctggctgct ggttgcccac tatcgcgaag agagcattgt
     5101 taaacgctgg caacaagatc cgctctggca gatgttaacc gccgcgcaga agcagcaggt
     5161 tgcttcggtc gacagtaaca cctgggcgcg gatgcgcggt atttttgctg cagagcgtat
     5221 tgccgctgac acggtaaaaa tcttccacca tcagccgctt accgttgtga aatgaccgcg
     5281 ataaaacacc cggtgctgct gtgggggctt cccgttgcag cacttattat tattttctgg
     5341 ctgagtctgt tttgctactc ggccattcct gtttccggag cagatgcaac ccgcgccctg
     5401 ctgcctggac acacgccaac gctaccagaa gcgctggtgc aaaaccttcg tttgccacga
     5461 agcctcgtcg ccgttctgat cggcgcaagc ctggcgctcg cgggcacgct gctgcaaacc
     5521 ctgacccaca acccaatggc ctctccttca ctgctcggca ttaacagcgg cgcggcgctg
     5581 gctatggcgc ttaccagcgc gctgagtccg acgccgattg caggctattc tctgtcgttc
     5641 atcgcggcat gcgggggcgg cgtgagctgg ctgctggtca tgaccgcagg aggcggattt
     5701 cgtcataccc atgacagaaa caaactgatc ctcgcgggta tcgcgctgtc ggccttttgt
     5761 atgggcctta cccgcatcac cctgctgctg gccgaagatc atgcttacgg catcttttac
     5821 tggctggcag gcggagtgtc ccacgcccgc tggcaggatg tctggcagct cttgccggtg
     5881 gtggtcactg cagtccctgt cgtgttgctg ctggcgaatc aactgaacct gctcaacctc
     5941 agcgacagca ccgcccatac gctgggagtg aacctgacga ggctacgttt ggtcatcaat
     6001 atgttagtgc tgcttctggt tggtgcgtgc gtcagcgtgg caggtccggt ggcgtttatc
     6061 ggtctgctgg tgccacatct ggcgcgcttc tgggcaggct tcgatcagcg caacgtactg
     6121 ccagtgagca tgctgctggg ggccacgctg atgctgctgg cagatgtact cgcacgcgcg
     6181 ctggccttcc ccggagatct gcccgcaggc gcagtgctgg cgctgattgg cagcccttgc
     6241 tttgtctggc ttgtgaggag gcgaggatga aaattgcgct ggttattttc atcacccttg
     6301 ccctggcggg ctgtgcgctg ttatcactcc atatgggagt gatccccgtg ccgtggcgcg
     6361 cgctgctgac cgactggcag gccggacgcg agcattatta tgtattgatg gagtaccgac
     6421 tgccgcgctt gctgttggca ctgtttgtcg gtgcagccct cgccgtggcg ggtgtgctga
     6481 tacaggggat tgtgcgcaac cctctggcat caccggatat tctcggcgtt aaccatgccg
     6541 ccagcctggc ctctgtgggg gctctacttc ttatgccgtc actgcccgtg atggtgctgc
     6601 cgctgctggc ctttgcgggc ggcatggcgg ggttgatatt gctgaagatg ctggcaaaga
     6661 cccaccagcc gatgaagctg gcgctcaccg gcgtggcgct ttctgcatgc tgggccagcc
     6721 tgacggatta tctgatgctc tcgcgcccgc aggatgtgaa caacgccctg ctgtggctga
     6781 ccggcagctt atggggccgt gactggagct ttgtgaagat tgccatcccg ctgatgattt
     6841 tatttctgcc gctgagcctg agtttttgcc gcgatctcga cctccttgca ctcggcgatg
     6901 cgcgcgccac cacgctcggt gtgtcggtgc cccatacccg attctgggct ttgttactag
     6961 ctgtcgccat gacatctacc ggcgtggccg cctgcggccc gattagcttt attggtctcg
     7021 tggtgccgca tatgatgcgt agcatcaccg gtggacgtca ccgcagactg ctgcctgttt
     7081 cggccctgac aggtgcgttg ctgttggtgg ttgccgatct gctggcgaga attatttatc
     7141 ccccactgga gctcccggtt ggcgtgctga ccgccattat cggtgcgccg tggtttgtct
     7201 ggttgcttgt gagaatgcga taaatgactt tacgaactga aaatctgacg gtcagttacg
     7261 ggacagacaa ggtacttaac gacgtttcac tctcactgcc aacggggaag atcaccgccc
     7321 tgatcggtcc taacggttgc gggaaatcga cgctgttaaa ctgtttttcg cggcttttaa
     7381 tgccgcagtc tggcaccgta tttctcggcg ataatcccat aaatatgctc tcatcgcgcc
     7441 agttggcccg caggctttcg ctgctgcctc agcaccattt aacgccagag gggatcacag
     7501 tccaggagct ggtttcgtat ggtcgtaatc cctggctgtc actctggggg catctctccg
     7561 ctgaagacaa tgcacgagtt aatgtcgcca tgaaccagac ccggattaat catcttgccg
     7621 ttcgtcggtt aaccgagctt tccggcggtc agcgccagcg cgcatttctg gcgatggtcc
     7681 tggcccagaa tacgcccgtt gtattacttg atgaaccaac cacctatctt gatatcaatc
     7741 accaggtgga cctgatgcgg ttgatgggcg aactccggac tcaggggaaa acggtggtcg
     7801 ctgtgctgca cgaccttaat caggctagcc ggtactgcga tcaactggtg gtaatggcaa
     7861 acgggcatgt tatggcgcaa ggcacaccag aagaggtgat gaccccagga ttgctgagaa
     7921 cagtattcag cgtggaagcg gaaatacacc ccgagccggt atctggcagg ccgatgtgcc
     7981 taatgaggta gattgcacag gccgtaagaa ccaaaccacg actgaatgaa actggactgg
     8041 cgccagcaag ccagttcaga ctggggctga acttttccgg actctgaaag attaccaata
     8101 ctcatcgtcc atccgcttgc tttaggctga caggttcata atcaacgcaa accagagctg
     8161 tacaggcttg ggcgcggctt tcaaaccagt cgtgaacggc aatcaatttt gaactctgct
     8221 taacggacat ttctgtataa cccttacggc aacgaaaaac gcgaagttaa aattttagaa
     8281 acccaaaaac gtgacatgac taagtttaga tttcaggggg gagatcaaaa aatttcgctc
     8341 tgtgccagag cggacattca tggagctggt tcattaccaa tgaggttggg cttttgagga
     8401 taaatcaatg atcagacgcc aacgtaaatc aaaagcaacc ctggaacgga attgctaatc
     8461 cagtttctga ccatcgattt ttctaaaaag tgtgcgtttg ctactactta ggtaggtgca
     8521 gctttcttaa tcaccggcag ccacgctggt ctgtttactg tcgtacttac tggtgtcctg
     8581 ctggctgctt atcagcaggt cgtggcccac atcggcgata atcctgttgc cgttgacctg
     8641 agcaccgttc agtaccgtat cccgaccact gttgatggtg acggttttac cgctgtctgt
     8701 tgtggtttca gtccactcag taccgttacc tttctcgctg ccttttgccg cattaacgct
     8761 ggcaaagacg ctgataccgg caccgttacc accggcaccg atactgacac ccacgccacc
     8821 gccagccccg gttatcaatg gtgctgaggt agtcactcca tgaatacgtg taatccacca
     8881 gggtatagcc atacggcaga ctgataccgg cggcaaaatt cctcgcatca tgtgacaccg
     8941 aaaagtcact gctccgtccc ccgctgacaa accagttgtc agccagcccc agaggattat
     9001 taaaggaaag gacaccattt aactgccccg taccggtatt cttctgcccg ctgttgtcga
     9061 tgcccacgct ccctgtgact ggccattccg gcaatgccgt cagtgtcacc accgaccatc
     9121 cctcacggtc accgggcgat atttcaatct gtaccggttc cgtacgcaga cgattaatct
     9181 gctccatccc ctgctcaata tcccgcaggt tcagaacctt cccctccatt cccgggaaaa
     9241 ccatcttcag ggtgcgggca ggaaggtcag cgccttccgc ccggatttgc tgcagcctgc
     9301 cttccatgac cgttatgtgc agtacgcccc ctgaaaggtc ctgctctgtc agaaaggccc
     9361 ggctggtgat atatccccgg cgtatatagc cgtccgtcac ggcatccgtg accgcagtca
     9421 ggccggtgat attcagacac tgattcaccc acggtgccac cagtctgtcg gtttctgcag
     9481 acgtcagtcg ggtggcccca ctgacaacaa tgcgtgacac cgtaaagcag gggccttcag
     9541 taccggcaga cgtttccgga gacggcgtga tagtcagcgg cgcactgcgc tccagcgcat
     9601 cacgctgatg ctggttttca tccagcaact gctgctgttg ctgctgaatt gcactgcggt
     9661 cacccggaga cagcatggct gccggagacg aaaatgcggg gataaaaaaa ccagaaaaaa
     9721 cggagaggga gagatagcat atccacggag agccacgccg gaacatatca caacagggga
     9781 cagacaacgc acgggataaa agggacgttt tcgtcagaat gttgttctgc cggcgctgca
     9841 tgttctcccc gtattctggc ctgtgtgatg ccagtgtaaa ttcatgtcaa aaagtgtcag
     9901 cgagaaaatt tacacctgaa aaagtctgtg tcatgaaaca gttttatgac aagacatcaa
     9961 aaaaatgtca aaaacgtgat ctttcttcag cttttcagaa tattgagtac caggaagtca
    10021 atatcaattc tgggacacat atgcatttac cattaaaacc gggcacaaca agctgttcag
    10081 tctcaatcca taccccgtgt tttatcaata tcagaataag aatatctgcg gataaataaa
    10141 aaaggaatgt cgagggagct taccgcagag ggctctccac ctcacaaacc atttacaaaa
    10201 atgataccgg catatctttg gtccctgtca caaccagaca ttgacaatcc aatataaccc
    10261 caaatatatt tcgggtaaga agtct
//
LOCUS       DAZFWP020000166        10232 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFWP020000166 DAZFWP020000000
VERSION     DAZFWP020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.166"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..337
                     /locus_tag="ACHK0Z_RS20640"
                     /old_locus_tag="pgaptmp_004128"
     CDS             <1..337
                     /locus_tag="ACHK0Z_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4504055.1"
                     /translation="WSAFEGVFATKNVLAFSRGDSVNINHSPHDGLIIINKGNEEVEG
                     AWPNKLQPGKYKNMGSNSVNIIIINTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKT
                     GEPLNYLYL"
     gene            673..1650
                     /gene="glaH"
                     /locus_tag="ACHK0Z_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             673..1650
                     /gene="glaH"
                     /locus_tag="ACHK0Z_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU4504056.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTFIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNIDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1670..2938
                     /gene="lhgO"
                     /locus_tag="ACHK0Z_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             1670..2938
                     /gene="lhgO"
                     /locus_tag="ACHK0Z_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU4504057.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRFDNCGKMLVATSPLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQD
                     RGGEIIYNAEVSALNEHKNGVVIRTSQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPRPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2961..4409
                     /gene="gabD"
                     /locus_tag="ACHK0Z_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             2961..4409
                     /gene="gabD"
                     /locus_tag="ACHK0Z_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4504058.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4423..5703
                     /gene="gabT"
                     /locus_tag="ACHK0Z_RS20660"
                     /old_locus_tag="pgaptmp_004132"
     CDS             4423..5703
                     /gene="gabT"
                     /locus_tag="ACHK0Z_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU4504059.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5941..7341
                     /gene="gabP"
                     /locus_tag="ACHK0Z_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             5941..7341
                     /gene="gabP"
                     /locus_tag="ACHK0Z_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU4504060.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7362..8024
                     /gene="csiR"
                     /locus_tag="ACHK0Z_RS20670"
                     /old_locus_tag="pgaptmp_004134"
     CDS             7362..8024
                     /gene="csiR"
                     /locus_tag="ACHK0Z_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU4504061.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8025..8474)
                     /gene="kbp"
                     /locus_tag="ACHK0Z_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             complement(8025..8474)
                     /gene="kbp"
                     /locus_tag="ACHK0Z_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU4504062.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8558..8716)
                     /locus_tag="ACHK0Z_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             complement(8558..8716)
                     /locus_tag="ACHK0Z_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4504063.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8899..9198
                     /locus_tag="ACHK0Z_RS20685"
                     /old_locus_tag="pgaptmp_004137"
     CDS             8899..9198
                     /locus_tag="ACHK0Z_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4504064.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9208..9732
                     /gene="ygaP"
                     /locus_tag="ACHK0Z_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             9208..9732
                     /gene="ygaP"
                     /locus_tag="ACHK0Z_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU4504065.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9779..10183)
                     /gene="stpA"
                     /locus_tag="ACHK0Z_RS20695"
                     /old_locus_tag="pgaptmp_004139"
     CDS             complement(9779..10183)
                     /gene="stpA"
                     /locus_tag="ACHK0Z_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU4504066.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2384 a         2608 c         2767 g         2473 t
ORIGIN      
        1 atggagcgca tttgagggtg tatttgcaac taaaaacgtt ctggccttta gtcgtgggga
       61 ttctgtgaac attaatcact ctcctcatga tggactaatt ataataaata aaggaaacga
      121 agaagttgaa ggtgcctggc ctaacaaatt gcaacctgga aaatataaaa acatggggag
      181 taatagcgtt aacattatta ttattaatac ccgaaaaatt atcccccctg gtaaagcatt
      241 tatgcttaga ggcggaactc taaatatcaa tattcctggg cgtagcgctc ttcttttagg
      301 gaaaacagga gaaccgctga actatctcta tttgtaattt aatgttatat ctgccccgct
      361 aaaacggggc agataatatg tttagtttac taacggtcat tttgcattga agccatttac
      421 tgttttttat cgaccagata atctgttctc tgatggtaac tccccctaac ctattgcttt
      481 agttattcat ttcctgtctc actttgcctt aataccctac gttaaatgtt actaatttgt
      541 tgcttttgat cacaataaga aaacaatatg tcgcttttgt gcgcattttt cagaaatgta
      601 gatattttta gattatggct acgaaatgag catcgccatg tcaccctaca tctcataaga
      661 ggatcgcttc tgatgaatgc actgaccgcc gtacaaaata acgctgtcga ttcaggccag
      721 gactatagcg gattcacttt catcccgtcg gcgcaatccc cgcgtctgct ggaacttacc
      781 ttcaccgaac agacgaccaa acagtttctc gaacaggttg ccgaatggcc cgtacaggcg
      841 ctggagtaca aatcctttct gcgttttcgg gtaggcaaaa ttcttgacga tctgtgtgcg
      901 aatcagctgc aaccattgct gttgaagacc ctgctaaacc gtgctgaagg tgcgctgttg
      961 atcaatgcgg tgggtatcga tgatgtggcg caggcagatg agatggtgaa gctggcaacg
     1021 gcggtagcgc atctgattgg tcgctcaaat ttcgatgcga tgagcggtca gtattacgcg
     1081 cgtttcgtgg tgaaaaatat cgataactca gacagctatc tgcgtcagcc gcaccgcgtg
     1141 atggaactgc acaacgacgg cacgtatgtc gaagagatca ccgattacgt gctgatgatg
     1201 aaaatcgatg agcaaaacat gcagggtgga aattcgttgc tgctgcatct cgatgactgg
     1261 gaacatctgg accactattt ccgccatcca ctggcgcgtc gcccgatgcg ctttgccgcg
     1321 ccgccgagca aaaacgtcag caaagatgtt ttccatccag tgttcgacgt tgatcaacag
     1381 ggtcgcccgg tgatgcgcta tatcgaccag ttcgtccagc caaaagattt tgaagaaggc
     1441 gtgtggttga gcgagctttc cgatgccatt gaaaccagca aaggcattct ttctgtaccc
     1501 gttcccgttg gcaaattcct gttgataaac aacctattct ggctgcacgg tcgcgaccgc
     1561 tttactccgc acccggatct gcgccgtgaa ctgatgcgtc agcgtggcta tttcgcttac
     1621 gcctctaacc actaccagac gcatcagtaa gcgcaaagga attgagcgga tgtatgattt
     1681 tgtgattatt ggcggcggca tcatcggcat gtcgaccgcc atgcaactga ttgatgtcta
     1741 tccggatgcc cgcattgcgt tgctggaaaa agagtccggc ccggcctgtc accagacggg
     1801 ccacaacagc ggcgtgatcc atgccggggt ctattacacg cccggtagcc tgaaggcaca
     1861 gttttgcctg gcgggaaacc gcgccactaa agccttttgc gatcaaaacg gcattcgctt
     1921 tgacaactgc ggcaagatgc tggtcgccac gtctccgctc gaaatggaac ggatgcgcgc
     1981 cttatgggaa cgcacagcgg cgaacggtat cgagcgcgaa tggctaaacg ccgacgaact
     2041 gcgcgagcgc gaaccgaata tcaccgggct cggcggtatt tttgtgccgt ccagcggcat
     2101 tgtcagctac cgcgaagtga cagcggcgat ggcaaaaatc tttcaggaca gaggcggcga
     2161 gattatttat aacgccgaag tcagcgccct taatgagcat aaaaacggcg tggtgatacg
     2221 taccagtcag ggcggtgaat atgaagcatc gacgctgatt agctgttccg ggctgatggc
     2281 tgaccggctg gtgaaaatgc tcggactcga accgggcttt atcatctgcc cgttccgtgg
     2341 cgagtatttc cgccttgcgc cggagcataa ccagattgtt aaccacctga tttaccccat
     2401 ccccgacccg gcaatgccgt ttttgggcgt gcatctcacc cgcatgatcg acggcagcgt
     2461 gaccgtcggg ccaaacgcgg tgctggcttt caaacgtgaa ggctatcgca agcgcgactt
     2521 ctcatttagc gacacgctgg aaattttggg ctcgtcgggg attcgccggg tgctgcaaaa
     2581 ccatctacgc tcaggactgg gcgagatgaa aaactcgctg tgcaaaagcg gctatctgcg
     2641 gctggtgcaa aagtattgtc cccggctttc gttaagcgat ctccagccca ggcccgccgg
     2701 tgtgcgggcg caggcggtat cgccggacgg caagctgatt gacgattttc tgtttgtcac
     2761 caccccgcgc acgatccaca cctgcaatgc gccctccccg gcagcgacat cagcaattcc
     2821 tattggtgcg catattgtca gcaaggtaca aacgctgttg gcaagccaga gtaaccccgg
     2881 acgcacgctg cgagcggcac gtagtgtgga tgccttacac gccgcattta atcaataacc
     2941 tttgaaaaca ggacgtaaca atgaaactta acgacagtaa cttattccgc cagcaggcgt
     3001 tgattaacgg ggaatggctg gacgccaaca atggcgaggt catcgacgtc accaatccgg
     3061 cgaacggcga caagctgggt agcgtgccga aaatgggcgc ggatgaaacc cgcgccgcta
     3121 tcgacgccgc caaccgcgcc ctgccagcct ggcgcgcgct caccgccaaa gaacgcgcca
     3181 ccattctgcg caactggttc aatttgatga tggagcatca ggacgattta gcgcgcctga
     3241 tgaccctcga acagggtaaa ccgctggctg aagcgaaagg cgaaatcagc tacgccgctt
     3301 cctttattga gtggtttgcc gaagaaggca aacgcattta tggcgacacc attcccggtc
     3361 atcaggccga taagcgcctg attgttatca agcagccgat tggcgttacc gccgccatca
     3421 cgccgtggaa cttcccggcg gcgatgatta cccgtaaagc cggtccggcg ctggcggcag
     3481 gctgcacgat ggtgctgaaa cccgccagtc agacgccgtt ctctgcgctg gcgctggcgg
     3541 agctggcgat tcgcgcgggc attccggctg gcgtatttaa cgtggtcacc ggttcggcgg
     3601 gcgcggtggg taacgaactg accagcaacc cgctggtgcg caaactgtcg tttaccggtt
     3661 cgaccgaaat tggccgccag ttaatggaac agtgcgcgaa agacatcaag aaagtgtcgc
     3721 tggagctggg cggcaacgcg ccgtttatcg tctttgatga tgccgatctc gacaaagccg
     3781 tggaaggcgc actggcctcg aaattccgca acgccgggca aacctgcgtc tgcgccaacc
     3841 gtttatacgt gcaggacggc gtgtatgacc gctttgccga aaaattacag caggcggtga
     3901 gcaaactgca catcggcgac gggctggata aaggcgtcac catcgggccg ctgatcgatg
     3961 aaaaagcggt agcaaaagtg gaagagcata ttgccgatgc gctggagaaa ggtgcgcgtg
     4021 tggtttgcgg cggtaaagcg gatgaacgcg gcggcaactt cttccagccg accattctgg
     4081 tggacgttcc agccaacgcc aaagtgtcga aagaagagac gttcggcccc ctcgccccac
     4141 tgttccgttt taaagatgaa gccgatgtga ttgcgcaagc caatgacacc gagtttggcc
     4201 ttgccgccta tttctacgcc cgtgatttaa gccgcgtctt ccgcgtgggc gaagcgctgg
     4261 agtacggcat cgtcggcatc aataccggca tcatttccaa tgaagtggcc ccgttcggcg
     4321 gcattaaagc ctcgggtctg ggtcgtgaag gttcgaagta tggcatcgaa gattacttag
     4381 aaatcaaata tatgtgcatc ggtctttaac tggagaacgc gaatgagcag caataaagag
     4441 ttaatgcagc gccgcagtca ggcgattccc cgtggtgttg ggcaaattca cccgattttc
     4501 gctgaccgcg cggaaaactg ccgggtgtgg gacgttgaag gccgtgagta tcttgatttc
     4561 gcgggcggga ttgcggtgct caataccggg cacttgcatc cgaaagtggt ggctgcggtg
     4621 gaagcgcagt tgaaaaaact gtcgcacacc tgcttccagg tgctggccta cgagccgtat
     4681 ctggagctgt gcgagattat gaatcagaag gtgccaggca attttgccaa gaaaacgctg
     4741 ctggtcacca caggttctga agcggtggaa aacgcggtga aaatcgcccg cgccgccacc
     4801 aaacgtagcg gcaccatcgc ttttagcggc gcgtatcacg ggcgcacgca ttacacgctg
     4861 gcgctgaccg gcaaggtgaa tccgtactct gcgggcatgg ggctgatgcc gggtcatgtt
     4921 tatcgcgcgc tttatccttg cccgctgcac ggcataagcg aggatgacgc tatcgccagc
     4981 atccaccgga tcttcaaaaa tgatgccgcg ccggaagata tcgccgccat cgtgattgag
     5041 ccggttcagg gcgaaggcgg tttctacgcc tcgtcgccag cctttatgca gcgtttacgc
     5101 gctctgtgtg acgagcacgg gatcatgctg attgccgatg aagtgcagag cggcgcgggg
     5161 cgtaccggca cgctgtttgc gatggagcag atgggcgttg cgccggatct taccaccttt
     5221 gcgaaatcga tcgcgggcgg cttcccgctg gcgggcgtca ccgggcgcgc ggaagtaatg
     5281 gatgccgtcg ctccaggcgg tctgggcggc acctatgcgg gtaacccgat tgcctgcgtg
     5341 gctgcgctgg aagtgttgaa ggtgtttgag caggaaaatc tgctgcaaaa agccaacgat
     5401 ctggggcaga agttgaaaga cggattgctg gcaatagccg aaaaacaccc ggagatcggc
     5461 gacgtacgcg ggctgggggc gatgatcgcc attgagctgt ttgaagacgg cgatcacaac
     5521 aagccggacg ccaaactcac cgccgagatc gtggctcgcg cccgcgataa aggcctgatt
     5581 cttctctcct gcggcccgta ttacaacgtg ctgcgcatcc ttgtaccgct caccattgaa
     5641 gacgctcaga tccgtcaggg tctggagatc atcagccagt gttttgatga ggcgaagcag
     5701 tagcgccgct cctatgccgg aggcgacgct gcgcgtcttg tccggcctac ggggatccag
     5761 gtcggataag gcgtttacgc cgcatccgac aatctgtacg tgaacaggaa gaaatctatg
     5821 ttggccgggt aaggcggagc cgctctccgg caaaaagaat caataacaat tatacgcgtg
     5881 acccggcgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag aggattcagg
     5941 atggggcaat catcgcaacc acatgagtta ggcggcgggc tgaagtcacg ccacgtcacc
     6001 atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc
     6061 gccgaagcgg gcccggcggt attactggcc tatctgttcg ccgggctact ggtggttatg
     6121 attatgcgga tgttggcgga aatggcggtt gccacgcccg ataccggttc gttttccacc
     6181 tatgccgata aagccattgg tcgctgggcg ggctatacca tcggctggtt gtactggtgg
     6241 ttttgggtac tggttatccc gctggaagcc aacatcgccg ccatgatcct gcactcatgg
     6301 gttccaggca ttcccatctg gttattttcc ctcgtcatta ccctcgcctt aactggcagt
     6361 aacttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc
     6421 atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggttt ttacccgtat
     6481 gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggtttc
     6541 ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc
     6601 accattgccg ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc caccaactcg
     6661 gttatctggc gtatttctat cttctattta tgttctattt ttgtcgtcgt ggcattaatt
     6721 ccgtggaata tgcccggact aaaagccgtc ggttcttatc gctcggtact ggaattgctc
     6781 aatattcccc atgcgaaatt aatcatggac tgcgtgatat tactttccgt aaccagttgc
     6841 ctgaactcgg cgctgtatac cgcgtcaagg atgctctact ccttaagccg tcgcggtgat
     6901 gctcccgcgg taatgggtaa aatcaaccgc agtaaaaccc cgtacgtggc ggtgttactc
     6961 tccaccggcg cggcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt
     7021 aaatttctga tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt
     7081 tcacagttgc ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg
     7141 tggctttatc cgtggctcac ctggctagtc atcggcttta ttacctttgt gttggtagtg
     7201 atgctattcc gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg
     7261 attatctgta ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca
     7321 cccgttcata atacgcgctg atcatgatca ggagtcacac catgaccatt acgtctctgg
     7381 atggctatcg ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat
     7441 tacgaatgag tttgctgaca tcgcgttatg cacttggcgt tgggccgtta cgggaagctc
     7501 tttcgcaact ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg
     7561 cgtctatgtc agagcaggag ttgctcgata ttttcgacgc ccgcgccaat atggaagcga
     7621 tgttagtgag tctggcgatt gcccgcggtg gcgatgagtg ggaggcagac gttctcgcaa
     7681 aagcgcatct gctgagtaag cttgaggcct gtgacgccag cgagaaaatg cttgatgagt
     7741 gggatctgcg tcatcaggcg tttcatacgg caattgtggc gggctgtggt tctcactatt
     7801 tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ctatcgattt atctggctgc
     7861 ggcgaacggt gctttcggta gaaatgctgg aggataaaca cgatcagcac cagaccctga
     7921 ctgcggcggt actggcgcga gataccgcgc gcgccagtga gttaatgcgc cagcatttac
     7981 tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc ttccggaata
     8041 cgcaacactt gccccggata aattttatcc gggcttttta gcatcggttt attcgcttcg
     8101 aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc actcagagtg
     8161 tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt tttcacctga
     8221 tcatcaacac tggcaatacc ggaaatattc ccaaccgcaa caaggatttt ctccttcgcc
     8281 tcctgactca ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg aatgttcact
     8341 ttatcggcat caggtatacc ggttttgctc agatgctcct gcaccttctt cgcctgatcg
     8401 tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa
     8461 ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg
     8521 aacgcggcgc gttaacgccg cgggggtgag cacaggttta atcgcgggtt tgcacccaga
     8581 acgcgtgaat caaaccgggg atatacccaa gcagtgtcag cagaatatta ataatgaacg
     8641 cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg cagaatgatg gtgatgacga
     8701 ttctccagaa acccatatgt actccctata agaaaattac tcattgttta aaaagagatt
     8761 ttatctctta agcgtagtaa ttttagcgga gcctgccagt ttcagcttac gcttttactc
     8821 tcatttacgc tgctgaggct ggtatatgtt gcatttttag tttagagttt acttaattta
     8881 gaaaatactt aaataaatat gactgaactc gcgcaattac aggccagtgc cgaacaggca
     8941 gcggccttat tgaaagcaat gagccatcct aaacgattgc tgattctgtg catgcttagc
     9001 ggttcccccg gcaccagcgc gggagagctg acgcgcatta ccggactgag tgcctctgcg
     9061 acatcacagc atctcgctcg tatgcgggac gaagggctta tcgacagcca acgggatgcc
     9121 caacgcattc tatattccat taaaaatgag gcggtaaatg ccattatcgc caccctgaaa
     9181 aatgtctatt gtccgtaagg agtcactatg gctttgacaa ccatttcgcc gcatgatgca
     9241 caagaattaa tcgcacgcgg cgcaaagtta atcgatattc gtgacgctga tgaatatctt
     9301 cgtgaacata ttcctgaagc agatctggct ccattatccg tgctggaaca gtcaggtctt
     9361 ccacctaaat tacgtcgcga gcaaattata ttccactgcc aggcaggtaa acgcaccagt
     9421 aataacgccg ataaattagc agcgattgcc gcccccgcag aaatattttt actcgaagat
     9481 gggattgatg gctggaaaag agctggattg ccagtagcgg taaataaatc tcaacccttg
     9541 ccgttaatgc gccaggtgca gatcgctgcg ggtggtttaa tattaatcgg cgttgtactg
     9601 ggttataccg taaatagcgg tttcttctta ttaagtggct ttgttggtgc cgggttactg
     9661 tttgcaggaa tcagcggttt ttgtggaatg gcaaggttgt tagataagat gccgtggaac
     9721 caacgaactt gagaagcgac gccggacgcg ccctagcagc gacatccggc ctcagtaatt
     9781 agatcaggaa atcgtcgaga gatttacctt ctgccagcgc ctgagcaatt ggcttcggtg
     9841 tacggccctg accggtccag gttttagttt caccgttaac atcggtgaat ttatatttcg
     9901 ccggacgcgg ctggcgtttt ttaccagcgc gtggtgcagc agcagagcta ttacctaata
     9961 actcttccgg gttaattccg tcagccttca tcagctccag ccaggtgcta attttttcct
    10021 gacgctcagc cagttcacgc tgctgctgtt cttcttcttc acgtctttct ttagtgacaa
    10081 ccctgaattt ttcgagcatt tcttcaagaa cgtcaatgga gaattcgcga gccatcgcac
    10141 ggagagtgcg aatgttattt aaactttgta acattacgga cataataaag aaaacctttt
    10201 aacgccaaaa caaaaaagta tttcgtcgcg ta
//
LOCUS       DAZFWP020000167        10162 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFWP020000167 DAZFWP020000000
VERSION     DAZFWP020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.167"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            148..336
                     /locus_tag="ACHK0Z_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             148..336
                     /locus_tag="ACHK0Z_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504067.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            333..1160
                     /locus_tag="ACHK0Z_RS20705"
                     /old_locus_tag="pgaptmp_004141"
     CDS             333..1160
                     /locus_tag="ACHK0Z_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4504068.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            1201..1572
                     /locus_tag="ACHK0Z_RS20710"
                     /old_locus_tag="pgaptmp_004142"
     CDS             1201..1572
                     /locus_tag="ACHK0Z_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGU4504069.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            1604..1708
                     /locus_tag="ACHK0Z_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /pseudo
     CDS             1604..1708
                     /locus_tag="ACHK0Z_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            1764..2018
                     /gene="xisR"
                     /locus_tag="ACHK0Z_RS20720"
                     /old_locus_tag="pgaptmp_004144"
     CDS             1764..2018
                     /gene="xisR"
                     /locus_tag="ACHK0Z_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4504070.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2052..3338
                     /locus_tag="ACHK0Z_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             2052..3338
                     /locus_tag="ACHK0Z_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4504071.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            3322..4118
                     /gene="yecE"
                     /locus_tag="ACHK0Z_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /pseudo
     CDS             3322..4118
                     /gene="yecE"
                     /locus_tag="ACHK0Z_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            4171..4566
                     /gene="yecN"
                     /locus_tag="ACHK0Z_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             4171..4566
                     /gene="yecN"
                     /locus_tag="ACHK0Z_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU4504072.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            4607..5350
                     /gene="cmoA"
                     /locus_tag="ACHK0Z_RS20740"
                     /old_locus_tag="pgaptmp_004148"
     CDS             4607..5350
                     /gene="cmoA"
                     /locus_tag="ACHK0Z_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU4504073.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5347..6318
                     /gene="cmoB"
                     /locus_tag="ACHK0Z_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             5347..6318
                     /gene="cmoB"
                     /locus_tag="ACHK0Z_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU4504074.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(6483..8912)
                     /gene="torZ"
                     /locus_tag="ACHK0Z_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             complement(6483..8912)
                     /gene="torZ"
                     /locus_tag="ACHK0Z_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU4504075.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(8937..10037)
                     /gene="torY"
                     /locus_tag="ACHK0Z_RS20755"
                     /old_locus_tag="pgaptmp_004151"
     CDS             complement(8937..10037)
                     /gene="torY"
                     /locus_tag="ACHK0Z_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4504076.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2573 a         2554 c         2398 g         2637 t
ORIGIN      
        1 tttactaacc atttatatga aaaacaaccg tgaattaagc agagtaaaac tgcttttaat
       61 ccttgccaca gtactgacaa taacagaaat cattattctc tttattgcgc tgtctgtcag
      121 ttaaaaatat cgggatacca cataccaatg agactgtatt tcacaatagt aattttactg
      181 gcaattatcg catgcattta cggattactc gttccgttcc ttatatccat gaaggatacg
      241 atagcagtta cttctggctt tgcactggcg tttctgaccc cgccctgcat ttatgccatt
      301 tacaagggtc tttctttcac taaggataaa aaatgaaaaa aattattttt gctttagcca
      361 ttgttctgcc gaccattggc cttgtcggct gcgatcgcgt tgaacccggt aatgttggca
      421 tcaaagtaaa taaactgggc gacgataaag gcgtcggtga ggtggtcggt gttggtcgct
      481 actggactgg ctggaatact gaagtttaca tcttcccaac cttcaaacaa atgaagacct
      541 acgatgagcc gttcagcttc cagatgagtg acggcacaac catcggctat cacatcggtg
      601 ttgcctacaa agttgatcca tccaaagtta ccacggtgtt tcagacctac cgcaaaggcg
      661 tggatgacat taccgccact gacctgcgcc agaagatcgc cgacgcactc aatcgactgg
      721 ccagcaaaat gaccaccgac aaatttatcg acggcggcaa gtctgaacta ctggatgccg
      781 cacttaaaga cattcaggaa gagatgaccc ccatcggcgt tcaggtaatg agcctctctt
      841 atgtaggtaa accggaatac ccgccaaccg ttatcgacag cattaacgcc aaagtcacgg
      901 caaaccagaa aaccctgcag cgcgaacagg aagtcaagca acgtgaagcg gaggccaaca
      961 tgctgcgtgc agaagctgcc ggacaggctg atgccattcg aacaaaagcc ctggcagaag
     1021 ccgatgccat tcgtttacgc ggcgaagctc tgcgccagaa ccctggcgtc atggagctgg
     1081 aagccatcaa caagtggaac ggtacactgc cgcaatacat gaccagtggt gccaatacac
     1141 catttatcca gattaaataa cttacacgcc cggcaaaccg ccgggttaat ggaaaatcag
     1201 atgaacaacc agaatactca accgcaaata atgaactatg acccgaatct gacgtcgtgc
     1261 ggacgcatgg caaaacaaac cgttcgatta actttcggac tatgggaata ccgcgaaaca
     1321 ttcgaagtta ctgtcggcgg caatctgacc ggactggatg ttatcaattg cgctattgaa
     1381 agcctgtacg caacgctgcc ttatgaagaa gtcctggatg agcgcacagg gaaaacggat
     1441 atcatggcca ccattaatat tggcgaactg atatgccagg atgaagacct gtccggggaa
     1501 ctctggcttg ccgggatgct tatctcagca gaaattatca gcattgaacc cgctacaaac
     1561 atacggctct gaagttctca ctattcagag agcaggagaa aaaatgttcg ctctgattaa
     1621 tcagggacaa ctgtataccg acagtgccgg ttacccggta aaaattgttc gctgcataaa
     1681 caacaccgtg ttgtacagaa gatgtgaagc gaatgtttga agtgtggtgc cactgccgtg
     1741 gatgaaagtt ttatgaggtt ggcatgcaga caatcatcta tcagataacc cccagcaaat
     1801 ggtgtacgga gagagtcctc attgcatcaa cagggctaaa gcctggcacc atcgagcgag
     1861 ccagaagaaa gtcatggatg cagggaaaag aataccgcca ttacgctgta gaaggtgatc
     1921 cggggcatta cagtgaatgc ctgtacaaca tcgaagaaat tatgcgatgg atcgaaaacc
     1981 agaaacaacc aggtgccaaa aatgcaagtt ccggttaacc tgttaatgct cctggacgtc
     2041 tgggaggttt tatgagtaac gcatcatacc cgacaggcgt tgaaaaccat ggaggatcac
     2101 tccgtatatg gtttcactat aatggcaaac gtgtcagaga aaacctcggt gttcctgaca
     2161 ccgccaaaaa ccggaagatc gctggtgagc ttcgcacttc tgtttgtttt gcaatcagaa
     2221 tggggagttt cgactacgcc gcgcagttcc ctaattcccc taacctgaaa cactttggtc
     2281 tgggaaaaag agagataacc gttaaggcac tttcggaaaa atggttggac cttaagaaaa
     2341 ttgagatagg aagtaatgca ttcagtcggt atcaatccgt ggtgagaaac atgcttcctc
     2401 gcatagggga aaaacgtctt gcttcgtcgg taacaaagga agatttactg tttatcagga
     2461 aagatttatt gaccgggtat cataatcttt ctaacggaaa aacaacgccg attaaaggga
     2521 ggtcagtagt tacggttaat tactacatga cgacaattgc aggaatgttt caatttgcgg
     2581 ctgataacgg ctatatcgtg tcaaacccat ttaacggcct gacaccatta aagagatcca
     2641 gaacagaacc agatccgctc acacgtgacg aatttattcg ttttattgat gcctgtcacc
     2701 atcaacaaac aaaaaacctg tggtccttag cagtatacac aggcattcgt cacggtgagc
     2761 taatatctct cgcctgggag gatattgatt taaaagctaa aacaatgact atccgtcgta
     2821 attatacaaa actcggggag ttcactctac caaaaacaga agcgggaact gatcgtgtta
     2881 ttcatcttgt tcaaccagct gttgatgccc tgaaaagcca ggctgaaatg acaagacttg
     2941 gccctcagta tcaaattgac gtcaagcttc gggagttcgg tcgcaccgca cgccatgaat
     3001 gcacgtttgt ttttaatccg caactggtaa aaaaatgcca gcaagtcggt caccactata
     3061 aagcagattc catcagagat tcctgggcat ctgcattaag gcgagcagga ctgcggcaca
     3121 gaaaagccta tcagtctagg catacttatg cctgctgggc attatcggca ggagcgaatc
     3181 caagcttcat agcaaaccaa atgggccatg caaatgcaca aatggtattc aacgtttacg
     3241 gagcatggat gaaagataac aatatcgggc aaatagaact actcaataag cagttgacgg
     3301 agagtgtccc atacatgccc catagagcca gactctgaaa taatatcttt aaatatcagt
     3361 tgttttagtg gtagttgccg tcacattaac tgcgtggagg gcaacaccac gctttacgcc
     3421 ctgccgaaac ccgaggttgt cctgcgctgg cgtgaacaga ccacagatga cttccgcttc
     3481 tgttttaagt ttccggcgac catttcgcat caggcagcat tacgacattg cgatgattta
     3541 gtgactgaat ttttgacccg catgtcaccg ttggctccgc gcattgggca atactggctg
     3601 caactgcctg ccacattcgg cccacgggag ctgcctgcgc tttggcattt tctcgattct
     3661 ctccctggcg aatttaatta tggtgtggaa gtccgccatc cacagttttt cgccaaaggg
     3721 gaagaggaac aaactcttaa tcgcggttta catcagcgcg gcgttaatcg ggtgatttag
     3781 acggccgccc ggttcatgca gcatgtccac acagtgaagc tattcgcgac gctcaacgaa
     3841 aaaaacctaa agttccggta catgctgtac tgacggcgac aaatccgctg atccgtttta
     3901 tcggtagtga tgatatgacg caaaaccggg aattatttca ggtctggtta cagaaattag
     3961 cgcagtggca tcagaccact acgccttatc tttttttaca tacgccagac attgcccagg
     4021 ccccggaact ggtacatacc ctgtgggaag acttacgtaa aacgcttcca gagatcggag
     4081 cagttccggc tattccacag caatcttctc ttttctgaat ttgccaccta tcatagacag
     4141 gtgccatcgg ccattttaaa gggagtttgt atggtaagcg cgctatatgc cgttttaagt
     4201 gcgttgttat taatgaagtt ctcttttgat gtcgttcgct tgcgtttgca gtaccgcgtt
     4261 gcctatggtg acggtggttt tagcgaactg caaagcgcta ttcgcattca tggtaacgcg
     4321 gtggaatata ttcccatcgc gattgtgctg atgctgttta tggaaatgaa tggcgcagaa
     4381 acctggatgg tgcatatttg cggcatcgtt ttgcttgctg gtcgtctgat gcattattac
     4441 ggttttcatc accgtctgtt tcgctggcga cgttccggca tgagcgccac ctggtgtgcg
     4501 ctgttgctga tggtgctggc gaatctttgg tatatgccct gggagttggt tttctccctg
     4561 cgttagcgca caatacgcca ctttcttttt cccggatttt tacgttatgt ctcaccgcga
     4621 cacgctattt tctgccccta tcgccagact gggcgactgg acctttgatg aacgggtagc
     4681 tgaagtcttc ccggatatga tccagcgttc cgttcccggc tattccaata ttatttccat
     4741 gattggtatg ttagccgagc gcttcgttca acctggtacg caggtttacg atctgggttg
     4801 ttctctgggc gcggcgacgc tctcggtgcg tcgcaacatt catcatgata attgcaaaat
     4861 tattgccatc gacaactccc cggcgatgat tgaacgctgc cgtcgtcata ttgacgccta
     4921 taaagcccct acgccagtag acgtcattga aggtgatatt cgcgatatcg ccattgaaaa
     4981 cgcatcgatg gtggtgctga attttaccct gcaattcctg gaaccttccg agcgccaggc
     5041 attactggat aaaatttatc aaggactgaa cccaggcggc gcgctggtgc tttcggaaaa
     5101 attcagtttc gaagatgcca aagttggtga actgttgttc aacatgcacc acgactttaa
     5161 acgtgccaac ggttacagcg aactggagat cagccagaag cgcagcatgc tggaaaacgt
     5221 gatgctgacc gattccgtgg aaacccataa agcacgcctg cataaagccg gttttgagca
     5281 tagcgagctg tggttccagt gctttaactt tggttcactg gtggcattaa aagcagagga
     5341 cgctgcatga tcgactttgg taacttttat tctctgattg ccaaaaatca tctttcacac
     5401 tggctcgaaa cgctgcccgc gcagattgct aactggcagc gcgagcagca gcacgggctg
     5461 tttaagcagt ggtccaacgc ggtggaattt ctgcctgaaa ttaaaccgta tcgtctggat
     5521 ttattgcata gcgtaaccgc cgaaagcgaa gagccactga gcaccgggca aattaaacgc
     5581 attgaaacgc ttatgcggaa cctgatgcca tggcgcaaag ggccgttctc actgtatggc
     5641 gtcaacatcg ataccgaatg gcgttccgac tggaaatggg atcgtgttct gccccatctt
     5701 tctgatttaa ccggacgcac cattctggat gtcggctgcg gcagcggtta tcacatgtgg
     5761 cgcatgattg gcgcaggcgc gcacctcgcg gtggggatcg accctacgca actgtttctg
     5821 tgccagtttg aagcggtgcg taaactactg ggtaacgatc agcgcgcaca tttgttaccg
     5881 ttaggtattg aacagcttcc ggcactgaaa gcctttgata ccgtcttttc gatgggcgtg
     5941 ctttaccatc gccgttcgcc gctggagcat ctctggcagt tgaaagatca actggtgaat
     6001 gagggtgaac tggtgctgga aacgctggtt attgatggcg atgagaacac ggtgctggta
     6061 ccaggcgatc gttacgcgca aatgcgtaat gtctatttca tcccttccgc gctggcgctg
     6121 aaaaactggc tgaagaagtg tggttttgta gatatccgcg ttgtggatgt gtgcgttacc
     6181 actactgaag agcagcgacg cactgaatgg atggttaccg aatctctgtc cgatttcctc
     6241 gacccacatg atccgagtaa gacagttgaa ggttatcctg cgcctaaacg cgcggtgctg
     6301 attgcgcgca agccgtaaag gtctggcatt actgccggat gcggcttgaa tgccttatcc
     6361 ggcctacaaa cccttgctaa ttcaatctat tgcagggact atgtaggcct gataagcata
     6421 gcgcatcagg cagttttacg tttgcataac ctcagcgccc gtttccgggc gctattcact
     6481 tcttatgcct gaatagctgg ctgatcaaac gccgttaact tcggcgcatt gcccgtatat
     6541 ttttcgatat acaccagcgc agagttaccg gcacaggcat ttgccagctg cgagctgggg
     6601 atatccgccg ttaacacgtt cgcactgccg tttttacaca agccattttc cagatctggc
     6661 catgcacctt catgcacgca taccacgcct tttttgatcc cgtcagtcac caccgcgcct
     6721 gtcagaatct gaccacgttt gttccacaca cgcaccagat caccattcgc aataccaaag
     6781 cgagcagcat cttcggtgtg aatagtgatt ggttcacgat ctgcaaccgc atatttttta
     6841 cgcagttccg catagttaag ctgactatgt aaacggtgtg ccggatgtgc ggtcagaagc
     6901 tgcaactgct tctcgtcggc ggtacccttc cactcatcag gcgcaagcca ggttgggtgt
     6961 gccgggcaat ccttatagcc aaatttttcc agcgttttgg agtaaatctc aattttgccg
     7021 cttggcgtac ccagcgcatt tttcaccgga tcggcgcgga aatcagcata acgaacgtac
     7081 tgttcgttct tctcgctgcg gcgcatttcg atcagtttat tttgctgcca gaaggcatta
     7141 aacatcggca tagtcacgcg ttgcgcacga gcacctttct gagcggcatc atagaaaaat
     7201 ttcagccacg ccatttcatc tttaccttcg gtatagatct cttttccgcc aggtttcagt
     7261 aattcagcaa gatcggcaaa tacgtcaaaa tcgttacgcg cttcaaattg cggagcaaca
     7321 gcctgcttca tcggcacaat atgctggttg ctgtaatcac cggtcatcgt caagtcattg
     7381 cgctcaaacg atgtggtgat cggtaatacg atatctgcat gtttagcagc tgcggtccag
     7441 tagcattcag aaacgacgat catctccggt ttctgccatg ctttgatcag acgattagta
     7501 tcctggtgat gggtaaagtt accgccaccc gcccaccaga tcattttgat attcggatac
     7561 gtttgttctt taccgttatg ctgatatttg ccaccaggat tttccagcgc atcgacgata
     7621 cgggcaacag gaatcgccgt cattccgcca tcatcagcgg cctcgctggc ctgtccggca
     7681 atcgccgcag acatttccgg cagcacgccg ccaacacgcg tcggattgcc accgttggag
     7741 taatgataag agaaaccaaa accgccgccc ggtgtaccaa tctgccctaa catcgccgcc
     7801 agcgtcacca gcatccagtg tttctgttca ccatattgct ggcgctgaat tccccagcca
     7861 gccatcagca ttgtgcgatt cgccgccatc agttcagcga gtttgactat ctgcgcttcc
     7921 ggtacgccag taatttccgc cgcccaggcc gcgctcttcg gagtattatc gctcttacct
     7981 gtcagatact cttcaaactg cggatacccg gtggtgtatt tttcaaggaa cactttatcg
     8041 tgtttacctt gtgtcatcag ggtatgcgca atccctaaca tcagtgccac gtcggtgccc
     8101 atattcggcg cgatccaggt agcattatca ccaaagaact cgatggtttc ggagcggata
     8161 ggatcaatgg caatcactgg tttgccagat tttttcagct gatggaagta ttccagccct
     8221 tgctcatcgg tactgctcca ggcaattttt aaggtattca gcgggttcat cccccacagc
     8281 accacagcct ggctgttttc cagaatcagc ggccaggagg tctgctgttc atacacctct
     8341 acagaaccga ccacatgcgg catgatcacc tgtgcagccc cggttgaata atcgccgcta
     8401 tgcccggaat aaccgcccgc caggttcata taacgttgca gtaaggtttg cgctttatgc
     8461 aacacgccag aagagcgcca gccgtatgat ccggcaaaaa tagcagatgg tccgttagct
     8521 ttgcgaatgc gatcgtgttg ctcatgaatc agttttaatg cttgctccca gctcacctgt
     8581 acataagtat cttcgccacg acctttcgcc ggttgcagtg gattatcgag atagcttttt
     8641 cttaccatcg gatgctgaat acgtgccgtg gtgtgtacct gatccgccgc cgtagactgt
     8701 aaggagttcg gtatggtttt cgccagcgcg cctgttgaag aaacaatctt gccgtccttc
     8761 acttctacgt tcatcgctcc ccaacgtccc gcggtgagga ttttaccgcc cttctcttct
     8821 gcccatgcgg ggagcggtgc tgccgatgtc accaccagcg ctccagcggc aataccgctg
     8881 tgtttaataa attcacgtct tgttaatgtc ataacttcct ccctgatcaa cgaggatcac
     8941 tgtttctcgg taatatcttt ggcgttgtac tggaaatacc gcgttaaaat gtccagttcg
     9001 ttttcgctca tgctggttcg cgcacccatt cctttggcaa tggacggcca tgcattgacg
     9061 gtgtaatggt cagcggcaat aggcgcatgg caaccagcgc aataggtatc gtcaagtttt
     9121 tcagcgtatt gccatagcgg tttacggtcc gctaatgcgg gatcggtaag cgcaccctgt
     9181 aaagacgcct gacgccattg attgccgtat tcgtcagcct gccattcccc gtttaccgtg
     9241 agcgccttga taccttcttc acttaatgtg gctagcgcca gccgttgacc tgccgccagg
     9301 tagagcgtgt tttcactgcc ctgcatttga taaccctgca acagaacgat cggctgtttg
     9361 ccactggcat caacgacggt gagatcggta ccaggattca cggtagccag ctcgcctatg
     9421 cgagaagttt tgaaaggata aatatgtgcg ccattagtca ctgaagtagc ggcctgactt
     9481 tccagctcat gcgccgcgtt gtcatccatt tttatttctg gcggaaaatg ggcaatgcct
     9541 ttatgacaat cgatacaggt ttcgccgccc ttttgtgctt tgttatgcat tttctgcgca
     9601 gattcacttt gcgaggcaat atccatggca tcaaaagaat ggcaactacg gcacgttgca
     9661 gagtcagttg cttttaattc tttccatact gtttcggcca tttcctggcg atgagtttcg
     9721 aacttatcgt cactgtctat tttgccgcta acaaattcat gataaatatc tttagatgct
     9781 tttaatttag caaataaata atccatccct gactttggaa tatggcaatc tgcacattcc
     9841 gcacgtatcc ctttctggtt cgaaaagtgg acagttccct gatattcctc aaaaggttta
     9901 ctcatcgagt ggcaggaaag gcaaaatgct gtatccgacg ttttatgtaa ggctttttgc
     9961 gccagcaata acccgccgcc accaaccaca accgctatca gcaaaaacaa taacccaatg
    10021 cgttttttcc ctcgcataga ttcctctaaa taaattccag aacaccgaaa tcattgtata
    10081 attaaataca caacgaatta accgcttgca taactcggca caacactgcc tgaaataatc
    10141 gataaagaat atgatttatt ac
//
LOCUS       DAZFWP020000168        10146 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFWP020000168 DAZFWP020000000
VERSION     DAZFWP020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.168"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(80..856)
                     /gene="yaaA"
                     /locus_tag="ACHK0Z_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             complement(80..856)
                     /gene="yaaA"
                     /locus_tag="ACHK0Z_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU4504077.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(926..2356)
                     /locus_tag="ACHK0Z_RS20765"
                     /old_locus_tag="pgaptmp_004153"
     CDS             complement(926..2356)
                     /locus_tag="ACHK0Z_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU4504078.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2635..3588
                     /gene="tal"
                     /locus_tag="ACHK0Z_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             2635..3588
                     /gene="tal"
                     /locus_tag="ACHK0Z_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4504079.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3703..4290
                     /gene="mog"
                     /locus_tag="ACHK0Z_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             3703..4290
                     /gene="mog"
                     /locus_tag="ACHK0Z_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU4504080.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4325..4891)
                     /gene="satP"
                     /locus_tag="ACHK0Z_RS20780"
                     /old_locus_tag="pgaptmp_004156"
     CDS             complement(4325..4891)
                     /gene="satP"
                     /locus_tag="ACHK0Z_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU4504081.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="ACHK0Z_RS20785"
                     /old_locus_tag="pgaptmp_004157"
     CDS             complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="ACHK0Z_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4504082.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5779..6183)
                     /locus_tag="ACHK0Z_RS20790"
                     /old_locus_tag="pgaptmp_004158"
     CDS             complement(5779..6183)
                     /locus_tag="ACHK0Z_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU4504083.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6560..8476
                     /gene="dnaK"
                     /locus_tag="ACHK0Z_RS20795"
                     /old_locus_tag="pgaptmp_004159"
     CDS             6560..8476
                     /gene="dnaK"
                     /locus_tag="ACHK0Z_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU4504084.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8565..9695
                     /gene="dnaJ"
                     /locus_tag="ACHK0Z_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             8565..9695
                     /gene="dnaJ"
                     /locus_tag="ACHK0Z_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU4504085.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="ACHK0Z_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="ACHK0Z_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU4504086.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2658 a         2545 c         2635 g         2308 t
ORIGIN      
        1 aattctgcaa tatattgaat ctgcatgctt ttgtaggccg gataaggcgt tcacgccgca
       61 tccggcattg gctacaaact taacgctgct cgtagcgttt aaacaccagt tcaccattgc
      121 tggaggaagc ttcatcaaag aagtaacctt cgctattaaa accagtcagt tgctctggtt
      181 tggtcagccg attttcaata atgaaacgac tcatcagacc gcgtgctttc ttagcgtaga
      241 agctgatgat cttaaatttg ccgttcttct catcgaggaa caccggcttg ataatctcgg
      301 cattcaattt cttcggcttc accgatttaa aatactcatc tgacgccaga ttaatcacca
      361 cattatcgcc ttgtgctgcg agcgcctcgt tcagcttgtt ggtgatgata tctccccaga
      421 attgatacag atctttccct cgggcattct caagacggat ccccatttcc agacgataag
      481 gctgcattaa atcgagcggg cgcagtacgc catacaagcc ggaaagcatt cgcaaatgct
      541 gttgggcaaa atcgaaatcg tcttcgctga aggtttcggc ctgcaagccg gtgtagacat
      601 cacctttaaa cgccagaatc gcctggcggg cattctccgg cgtgaaatct ggctgccagt
      661 catgaaagcg agcggcgttg atacccgcca gtttgtcgct gatgcgcatc agcgtgctaa
      721 tctgcggagg cgtcagtttc cgcgcttcat ggatcaactg ctgggaattg tctaacagct
      781 ccggcagcgt atagcgcatg gtggtcaacg ggctttggta atcaagcgtt ttcgcaggtg
      841 aaataagaat cagcatatcc agtccttgca ggaaatttat gccgacttta gcaaaaaaag
      901 agaatgagtt gatcgatagt tgtgattact cctgcgaaac atcatcccac gcgtccggag
      961 aaagctggcg accgatatcc ggataacgca acggatcaaa caccgggcgc acgccgagtt
     1021 tacgctggcg tagataatca ctggcaatgg tatgaaccac aggcgagagc agtaaaatgg
     1081 cggtcaaatt ggtaatagcc atgcaggcca ttatgatatc tgccagttgc cacatcagcg
     1141 gaagacttag caaggtgccg ccgatgaccg ttgcgaaggt gcagatccgc aaacaccaga
     1201 tcgctttagg gttgttcagg cgtaaaaaga agagattgtt ttcggcataa atgtagttgg
     1261 caacgatgga gctgaaggca aacagaataa ccacgagggt aacaaactca gcaccccagg
     1321 aacccattag cacccgcatc gccttctgga taagctgaat accttccagc ggcatgtagg
     1381 ttgtgccgtt acccgccagt aatatcagca tggcgcttgc cgtacagatg accagggtgt
     1441 cgataaaaat gccaatcatc tggacaatcc cttgcgctgc cggatgcgga ggccaggacg
     1501 cagctgccgc tgctgcgttt ggtgtagaac ccattcccgc ctcattggaa aacatactgc
     1561 gctgaaaacc gttagtaatc gcctggctta aggtatatcc cgccgcgccg cctgccgctt
     1621 cctgccagcc aaaagcactc tcaaaaatag accaaatgac gtggggaagt tgcccgatat
     1681 tcattacgca aatcaccagg ctggtcagta cccagattat cgccatcaac gggacaaaac
     1741 cctgcatgag ccgggcgacg ccatgaagac cgcgagtgat tgccagcaga gcaaagacag
     1801 cgagaataat gcctgtcacc agcgggggaa aatcaaaaga aaaactcagg gctcgggcaa
     1861 cggcgttcgc ctgaactccg ctgaaaatta tgccgtaggc gatgagcaaa aagacggcga
     1921 acagaacgcc catccaacgc atccccagcc cgcgcgccat ataccatgcc ggtcctccac
     1981 gaaactgtcc attgacgtca cgttctttat aaagttgtgc aagggaacat tcggcaaacg
     2041 aggtcgccat gccgataaac gcggcaaccc acatccaaaa gacggctcca ggtccaccgg
     2101 cggtaatagc cagcgcaacg ccggccaggt tgccgctacc cacgcgcgcc gcaagactgg
     2161 tacacaatga ctgaaatgag gttaaaccgc ctggctgtgg atgaatgcta tttttaagac
     2221 ttttgccaaa ctggcggatg tagcgaaact gcacaaatcc ggtgcgaaaa gtgaaccaac
     2281 aacctgcgcc gaagagcagg taaatcatta ccgatcccca aaggacgctg ttaataaagg
     2341 agaaaaaatc tggcatgcat atccctctta ttgccggtcg cgatgacttt cctgtgtaaa
     2401 cgttaccaat tgtttaagaa gtatatacgc tacgaggtac ttgataactt ctgcgtagca
     2461 tacatgaggt tttgtataaa aatggcgggc gatatcaacg cagtgtcaga aatccgaaac
     2521 agtctcgcct ggcgataacc gtcttgtcgg cggttgcgct gacgttgcgt cgtgatatca
     2581 tcagggcaga ccggttacat ccccctaaca agctgtttaa agagaaatac tatcatgacg
     2641 gacaaattga cctcccttcg tcagtacacc accgtagtgg ccgacactgg ggacatcgcg
     2701 gcaatgaagc tgtatcaacc gcaggatgcc acaaccaacc cttctctcat tcttaacgca
     2761 gcgcagattc cggaataccg taagttgatt gatgatgctg tcgcttgggc gaaacagcag
     2821 agcaacgatc gcgcgcagca gatcgtggac gcgaccgaca aactggcagt aaatattggt
     2881 ctggaaatcc tgaaactggt tccgggccgt atctcaactg aagttgatgc gcgtctttcc
     2941 tatgacaccg aagcgtcaat tgcgaaagca aaacgcctga tcaaactcta caacgatgct
     3001 ggtattagca acgatcgtat tctgatcaaa ctggcttcta cctggcaggg tatccgtgct
     3061 gcagaacagc tggaaaaaga aggcatcaac tgtaacctga ccctgctgtt ctccttcgct
     3121 caggctcgtg cttgtgcgga agcaggcgtg ttcctgatct cgccatttgt tggccgtatt
     3181 cttgactggt acaaagcgaa tactgataag aaagagtacg ctccggcaga agatccgggc
     3241 gtggtttctg tatctgaaat ctaccagtac tacaaagagc acggttatga aacagtggtt
     3301 atgggcgcaa gcttccgtaa catcggcgaa attctggaac tggcaggctg cgaccgtctg
     3361 accatcgcac cggcactgct gaaagagctg gcggagagcg aaggggctat cgaacgtaaa
     3421 ctgtcttaca ccggcgaagt gaaagcgcgt ccggcgcgta tcactgagtc cgagttcctg
     3481 tggcagcaca accaggatcc aatggcagta gataaactgg cggaaggtat ccgtaagttt
     3541 gctattgacc aggaaaaact ggaaaaaatg atcggcgatc tgctgtaatc attcttagcg
     3601 tgaccgggaa gtcggtcacg ctacctcttc tgaagcctgt ctgtcactcc cttcgcagtg
     3661 tatcattctg tttaacgaga ctgtttaaac ggaaaaatct tgatgaatac tttacgtatt
     3721 ggcttagttt ccatctctga tcgcgcatcc agcggcgttt atcaggataa aggcatccct
     3781 gcgctggaag aatggctgac atcggcgcta accacgccgt ttgaactgga aacccgctta
     3841 atccccgatg agcaggcgat catcgagcaa acgttgtgtg agctagtgga tgaaatgagt
     3901 tgccatctgg tgctcaccac gggcggaact ggcccggcgc gtcgtgacgt aacgcccgat
     3961 gcgacgctgg cagtagcgga ccgcgagatg cctggctttg gtgaacagat gcgccagatc
     4021 agcctgcatt ttgtaccaac tgcgatcctt tcgcgtcagg tgggcgtgat tcgcaaacag
     4081 gcgctgatcc ttaacttacc cggtcagccg aagtctatta aagagacgct ggaaggtgtg
     4141 aaggacgctg cgggtaacgt tgtggtacac ggtatttttg ccagcgtacc gtactgcatt
     4201 cagttgctgg aagggccata cgttgaaacg gcaccggaag tggttgcagc attcagaccg
     4261 aagagtgcaa gacgcgacgt tagcgaataa aaaaatcccc ccgagcgggg ggatctcaaa
     4321 acaattagtg ggattcacca atcggcagaa cggtgcgacc aaactgctcg ttcagtactt
     4381 cacccatcgc cagatagatt gcgctggcac cgcagatcag cccaatccag ccggcaaagt
     4441 ggatgattgc ggcgttaccg gcaatgttac cgatcgccag cagggcaaac agcacggtca
     4501 ggctaaagaa aacgaattgc agaacgcgtg cgcctttcag cgtgccgaag aacataaaca
     4561 gcgtaaatac gccccacaga cccaggtaga caccaaggaa ctgtgcattt ggcgcatcgg
     4621 tcagacccag tttcggcatc agcagaatcg caaccagcgt cagccagaaa gaaccgtaag
     4681 aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta ctccagcaga ccagcaaaaa
     4741 tttgcgcgat gccgccgtag aaaatgccca tggcaagaat aataccgtcc agagcgaaat
     4801 aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc gaagcccatc aggcccagcg
     4861 gtgccggatt agccaactta gtgttgccca taattcctca aaaatcatca tcgaatgaat
     4921 ggtgaaataa tttccctgaa taactgtagt gttttcaggg cgcggcataa taatcagcca
     4981 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
     5041 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
     5101 gtcacgcgat aggcgctgcc gctgaccgct ttaaccccat ttagtgccgc acctacaggg
     5161 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
     5221 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca cgccttttgc caataatttg
     5281 tctttcatca gcagcggcag cagctcttcc agctcattca ccctggcatc gatcgcgtgc
     5341 agaaactcct gcttatgttc ctcgtccata ttcttccagg tgttacgcag aaattgttcc
     5401 agtaactgtt gctcaatctc aaacgtagac atctctttgt cggctttcag cttcaatcgt
     5461 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
     5521 atactatcgc caccaaaatg ctgtaattct ccagcaatca gctgccagtt gcggcgatgt
     5581 tgctcgggat gcccttccat cgatttaaac agttcgttgc gcatcagtac gctggagagg
     5641 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
     5701 ctgcaatgct ggagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
     5761 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
     5821 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcgct
     5881 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcaggct
     5941 ctgactggcg ctacgggcgg ctttgaaata aaccgatgca ccgcttaact gtaaatcgcc
     6001 atggtcggca gagagttgta tgcgtttaac aattcggcaa acaggaagtt tcaacgccag
     6061 atcgttggtt tcgttacgcg gcattgcaat gacgccgagg attttatggt cgtttgcctg
     6121 cgccgtgcag cacagcatca gactaatcgc caggctggcg gaaatcgtaa aaacggattt
     6181 cataaggatt ctcttagtgg ggagaggtag ggggatggat acccactagt ttactgctga
     6241 taaagagaag attcaggtgc gtaatgtttt ctttttatta caattttttg atgaatgtct
     6301 tggctgcgat tcattcttta tatgaataaa attgctgtca gtttcacgtc ttgtcctgct
     6361 atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt
     6421 gggttacgac cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatgaaattg
     6481 ggcagttgaa accagacgtt tcgcccctat tacagactca caaccacatg atgaccgaat
     6541 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
     6601 ttgtgtagcg attatggatg gcaccactcc tcgcgtgctg gagaacgccg aaggcgatcg
     6661 caccacgcct tctatcattg cctataccca ggatggtgaa actctggttg gtcagccggc
     6721 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
     6781 ccgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
     6841 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
     6901 gatctctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc
     6961 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
     7021 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
     7081 agctgcgctg gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga
     7141 cctgggtggt ggtactttcg atatttctat tatcgaaatc gacgaagttg acggcgaaaa
     7201 aaccttcgaa gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag
     7261 ccgtctgatc aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
     7321 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
     7381 ttccgctcag cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa
     7441 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ctggttgaag atctggtaaa
     7501 ccgttccatt gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat
     7561 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     7621 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     7681 tgctgctgtt cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt
     7741 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     7801 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     7861 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     7921 gggtcagttc aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     7981 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     8041 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     8101 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     8161 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     8221 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac
     8281 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt
     8341 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     8401 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     8461 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
     8521 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
     8581 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca
     8641 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga
     8701 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
     8761 acgatcagta tggtcatgct gcgtttgagc aaggtggtat gggcggcggc ggttttggcg
     8821 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
     8881 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
     8941 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
     9001 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
     9061 gttctggtca ggtgcagatg cgtcagggat tcttcgctgt acagcagacc tgtccacact
     9121 gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
     9181 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
     9241 tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc accggcaggc gatctgtacg
     9301 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctctattgcg
     9361 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
     9421 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac cggtaagctt ttccgtatgc
     9481 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg
     9541 tcgtcgaaac accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag
     9601 aaagcttcgg tggcccaacc ggtgagcaca acagcccgcg ctcaaagagc ttctttgatg
     9661 gtgtgaagaa gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc
     9721 aggcctgggt aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat
     9781 cactccccgc cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
     9841 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc
     9901 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
     9961 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat agggggcctc
    10021 gggttgatgg taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
    10081 gacagacagc ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttctgtttta
    10141 tcgatt
//
LOCUS       DAZFWP020000169        10027 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFWP020000169 DAZFWP020000000
VERSION     DAZFWP020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.169"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..149)
                     /locus_tag="ACHK0Z_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /pseudo
     CDS             complement(1..149)
                     /locus_tag="ACHK0Z_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(<1..84)
                     /locus_tag="ACHK0Z_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(535..1728)
                     /locus_tag="ACHK0Z_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             complement(535..1728)
                     /locus_tag="ACHK0Z_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4504087.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1864..3588
                     /gene="iucA"
                     /locus_tag="ACHK0Z_RS20820"
                     /old_locus_tag="pgaptmp_004164"
     CDS             1864..3588
                     /gene="iucA"
                     /locus_tag="ACHK0Z_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HGU4504088.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3589..4536
                     /gene="iucB"
                     /locus_tag="ACHK0Z_RS20825"
                     /old_locus_tag="pgaptmp_004165"
     CDS             3589..4536
                     /gene="iucB"
                     /locus_tag="ACHK0Z_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HGU4504089.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4536..6278
                     /gene="iucC"
                     /locus_tag="ACHK0Z_RS20830"
                     /old_locus_tag="pgaptmp_004166"
     CDS             4536..6278
                     /gene="iucC"
                     /locus_tag="ACHK0Z_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HGU4504090.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6275..7612
                     /gene="iucD"
                     /locus_tag="ACHK0Z_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             6275..7612
                     /gene="iucD"
                     /locus_tag="ACHK0Z_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HGU4504091.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7618..9813
                     /gene="iutA"
                     /locus_tag="ACHK0Z_RS20840"
                     /old_locus_tag="pgaptmp_004168"
     CDS             7618..9813
                     /gene="iutA"
                     /locus_tag="ACHK0Z_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HGU4504092.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2369 a         2620 c         2659 g         2379 t
ORIGIN      
        1 gaccccgtac tgctcacgca gcttatccag cagtggcatc attttttcca gaggcggtcg
       61 aactccgcct tcgcaaaata agcggaagcc tggcgaagga tatcgttact gcggcgcagt
      121 tcacgatttt cacgctccag ctctttcagg tgtcaacgcc actctgagag agttttatgc
      181 atcccgtatt gggcactatg atgccggaaa tctgcgaggc tcgacacctc tctgattttc
      241 ccctttattt atcacggtgc cccagatcta atgttgggga tgcgttcatc tcattcccaa
      301 tgagtaatcg cactgttctc attgtctgca ggcatccgga cagagccatg tgatgttgca
      361 ctcacaacca gcatgacttc aatgcaactt aagtgacacc ggccggtttt ataccgaaca
      421 taccctgtac catgcggtgc atttttgcca gtgttactgc ctgacatgct ttcatcagac
      481 gcaggttctc tctcatatcc tctgagttac tccggtaagg tgatgatcat atctttattt
      541 gatagcacgg taatgccaga taaatacaga cacaatggta aataatatag ttgcaaataa
      601 tgccgccata atcagcgcga ttgcgacacc tgttttcgat accagcggca ccagcaaagc
      661 gcacagtcca tagccaagtg tgtgactggt agcgagccag cccgcgcctg cgccatcagc
      721 cagcttatca ttcagtagaa gttgataagc gggtgttgcc agagctgctc caaatgacag
      781 cactgcacag cccgggtaaa acaaccatag ctgttcggag agcatgatag ccagaccacc
      841 actcatcaac actccggcac tcaggagcag ggccaccgga gtcaggcgct gcggacggag
      901 taccccgaac tgcgcgataa gcgcagctac tgcggacaga cccaacagcc aggccacctg
      961 ttggctaatg gcggtggtat ccgtggcgaa ctggcgagta agggcaggcg aaagtccaag
     1021 ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa agcagataag gcagacaatc
     1081 ccgcttcaga ctgacgctct tgcgctccgg tgtgggctgt ggtggtgtgc cgggcaaccg
     1141 cagcagcatc agcagcgcca gcactggggc tgccatcagc agccccagtg gcgccagagg
     1201 atgaatggcc agcattgctg ccgcgcacag cggcccgaat agcctgccgc aactcaggcc
     1261 ggagctgatg gttgccagag cggccatgcg attcccttct cctgctctct gcaatgccca
     1321 gacctgacag gctggcacca ttgccgacac ggtcagcccg taggcgatcc gggcgatgat
     1381 taatattccc agtcccaccg ctgttgttat ggcgctggta gccatcagca cgcttcccag
     1441 ccctagcagg gtaaagctta tgccatatcc tcccagagcc cacaacacca caggtttact
     1501 gccagtacgg gaaatttgct ttccccacca cggggacgat ggcagaaaca gcattgagcc
     1561 aagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     1621 gagcacaacc agcagaccgt tctgcccgat accgagaagc ccggcactga acgcaagtgg
     1681 ccagcaggac agtggttttt ggggcgtatc ttcgatccca ggtgacattt taatgtttca
     1741 actccatgta ttaattgtgt ttatttgtaa aattaattta tctgacaata acatttctta
     1801 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtgg gagctgtttg
     1861 actatgaccc tgccctctga aaaaccagcc acagatgtgg ctgcgcagtg cttcctgaat
     1921 gcactgattc gtgaaactac agactggaaa ctgacagaat acccgccaga cgaattgatc
     1981 atcccgctgg atgagcagaa atcgctccat ttcagagtgg cttatttctc cccaacccag
     2041 catcaccgct ttgcatttcc tgcacgtctg gtcacggcat caggcagtta tcctgtcgac
     2101 tttaccactc tctcccggct tattattgat aagctacggc atcaactgtt tctgcccgtt
     2161 cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc acgcccatac gcaacaggca
     2221 attgatgccc gtcatgactg gaccgccctg cgtgaaaaag cgttgaattt tggcgaagct
     2281 gagcaggcgc tgctgacagg acacgctttc caccctgcgc ctaagtctca tgaaccgttt
     2341 aaccggcggg aggctgaacg ctacctgcct gatatggcac cccacttccc actgcggtgg
     2401 ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc atctcaacct tcaacagcgg
     2461 ctgacgcgat ttgccgcaga gaatgcgcct cagttactca acgaattaag tgacaaccaa
     2521 tggctgttcc cgttgcaccc gtggcagggg gaatatcttt tgcagcaggg gtggtgccag
     2581 gcacttgttg ctaaggggct gattaaagac ttaggtgagg ccggcacgtc gtggctgccg
     2641 accacctctt cccgttccct ctactgtgcc accagccgcg atatgatcaa gttctccctg
     2701 agcgtacggc tgaccaactc catccgtact ctgtccgtga aagaagtgaa gcgtgggatg
     2761 cgcctggcac gcctggctca aaccgacggc tggcagatgc tacaggtccg cttcccgact
     2821 ttccgggtaa tgcaggagga tggctgggcc gggctgctcg atcttaacgg caacatcatg
     2881 caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg atcagccgaa aagccagacc
     2941 aacgtactgg tctccctgac tcaggccgca cctgatggcg gtgattcgct gctggtttcg
     3001 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccatgcatgg
     3061 gtcgatgcat actgtcagca agtgctaaag ccgctgttta cggctgaagc ggattacggc
     3121 ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga tgcttgggga tctgccggtc
     3181 ggatttattt accgtgactg tcagggcagc gcttttatgc ctcacgcgac agactggctc
     3241 gattctattg gcgaggcgca ggcggaaaat attttcaccc atgagcagtt gctgcgctat
     3301 ttcccttatt acctgctggt taactccact tttgctgtga ccgctgcgct gggggctgcc
     3361 gggctggaca gcgaatcgaa tctgatggct cgtgtacgag catcgctggc tgaagtgcgt
     3421 gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg aaagtccgta ctggaacgta
     3481 aaaggtaact ttttctgtta tctgaacgat cataacgaga acaccattgt tgacccttcg
     3541 gtgatctatt tcgatttcgc taacccgctg caggctcagg aggtctgaat gtctgaggca
     3601 aacattattc acagcagata tggactgcgc tgtgaaaaac tcgacaagcc cctgaatctt
     3661 ggctggggac tggacaatag cgcggtgttg cactgtcccg gggagctgcc gacagggtgg
     3721 ctgtgcgatg cgctggatca gatatttatc gccgcaccac aactgtcagc agtggctctc
     3781 ccctgggccg aatggtgtga ggagccacag gcgctgacgc tttttgggca ggtaaaaagc
     3841 gacattatcc atcgtaccgc cttctggcag ttaccgttat ggttgagttc accggcaaac
     3901 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgccca
     3961 ccccgtccgc agggtgaggt ttaccgccgt tacgatccac gtattcgcag gatgctgagt
     4021 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca cccgctggat gaatgatccg
     4081 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagaccgc ctatctggag
     4141 cgccagctca ccggtaaaca tgcattcccg ctgattgggt gcttcgacga tcggccattc
     4201 agctattttg aaatctactg ggcggcggaa gaccgcattg gtcgtcacta ttcgtggcag
     4261 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg ggcccactat
     4321 gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc tggatgagcc ccgtacgcag
     4381 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     4441 ggataccgga caattaaaga gttcgacttc ccacataagc gctcgcgcat ggtgatggcg
     4501 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaggac tgggattttg
     4561 ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga gtatgagcag gttttccacg
     4621 cggaatctca gggcgatgac cactactgca ttaacctgcc gggagcacaa tggcgcttta
     4681 tcgctgaacg tggtatctgg ggctggctgt ggattgatgc ccaaacgttg cgctgcacgg
     4741 acgaaccagt actggcgcag acgctgctga tgcagctaaa gccggtgctg tcaatgagtg
     4801 atgccactgt tgcagagcat atgcaggatt tgtatgccac gctactgggc gacctgcaac
     4861 tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat taatcttgat gccgatcgtc
     4921 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     4981 gcaaagaggc gctggaacga tatgcgccag agtatgccaa caccttcaga ctgcactggc
     5041 tggcggtaaa acgtgaacat atgatctggc gctgcgacaa cgatctggat attcagcagt
     5101 tgttgactgc cgcaatggat ccgcaggagt ttacacgctt cagtcaggtc tggcaggaaa
     5161 acgggctgga tcacaactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     5221 tcgccaccga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     5281 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagc cgccggggag
     5341 ggctggatat caagctgccg ctgaccatct acaacacctc atgctaccgg gggatccctg
     5401 gcagatatat cgctgccgga ccactggctt cacgctggct gcaacaggtt tttgcgaccg
     5461 acgccaccct ggtgcaaagc ggcgcagtga tccttggtga accggctgca ggctatgtgt
     5521 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     5581 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtccg gttctgatgg
     5641 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atcgaccgct
     5701 ccggactgga cgctgaaacc tggcttacgc aattgttccg ggtggttgtg gttccgctgt
     5761 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca cggacaaaat ataacgctcg
     5821 ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga cttccagggc gatatgcggc
     5881 tggtgaaaga agcgtttccc gaaatggact ctttgcctca ggaggtgcgt gatgtcacat
     5941 cccgtctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     6001 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     6061 tgctggcagc agtgttgagt gattacatga acaaacatcc acaaatgtca gagcgttttg
     6121 cgcttttctc actcttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     6181 cctggccgga ccaggatggc ggcagtcgca tgctgccgaa ttaccttgag aatctgcaaa
     6241 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     6301 tgtcgggaca gggccattta atctcagtat tgctgcattg tcacatcaga tcgaagaact
     6361 gaactgtctc ttctttgacg aacatcctca tttttcctgg catccgggta tgctggtacc
     6421 ggattgtcat atgcagactg tctttctgaa agatctggtc agtgcagttg cacctacaaa
     6481 tccctacagc tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     6541 cagactacgt acagtatccc gtgaagagtt ttctgactat ctccgctggg ctgctgaaga
     6601 tatgaataac ctgtatttca gtcataccgt tgaaaatatt gatttcgaca aaaaaagtcg
     6661 attgtttctg gttcagacca gtcggggaga atattttgcc cgcaatatct gccttggtac
     6721 aggaaaacaa ccttatttac caccctgtgt gaagcatgtg acacaatcct gtttccatgc
     6781 cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa cggataactg tggttggtgg
     6841 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     6901 ggaaataaac tgggtctcac ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     6961 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactgaagg aagatattcg
     7021 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     7081 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     7141 tctgctaccc agccgctcgg taacaactct ggaaagtagt ggtccgggct ggaagttatt
     7201 gatggaacat catctggatc ggggcagaga gagcctggaa agtgatgtgg tgattttcgc
     7261 cacaggttac cgttctgcat tgccacaaat acttccctca ctgatgccct tgatcaccat
     7321 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     7381 agaaaataac atctttgcgg tcaacgccag catgcaaact catggcatcg ccgaacccca
     7441 gctcagcctg atggcctgga gatctgcacg tattcttaat cgcgtactgg ggcgtgattt
     7501 attcgatctc agtatgccgc ccgcactgat tcagtggcgc agcggcagcc ggaaaaaacc
     7561 gcagccggag gctgctgctt taactcacta tacaacaaat attcaggaat aacgatgatg
     7621 cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc ttgtcgtgct gtcaccagct
     7681 gtcgcccagc aaaacgatga taatgagatc atagtgtctg ccagccgcag caatcgaact
     7741 gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg ccgaactgga gcagcagatt
     7801 cagggcggta aagagctgaa agacgcactg gctcagttaa tccccggcct tgatgtcagc
     7861 agccagagcc gaaccaacta cggtatgaac atgcgtggcc gcccgctggt tgtcctgatt
     7921 gacggtgtgc gcctcaactc ttcacgttcc gacagccgac aactggactc tgtcgatcct
     7981 tttaatatcg accatattga agtgatctcc ggcgcgacgg ccctgtacgg tggcgggagt
     8041 accggagggt tgatcaacat cgtgaccaaa aaaggccagc cggaaaccat gatggagttt
     8101 gaggctggca caaaaagtgg ctttaacagc agtaaagatc acgatgagcg cattgccggt
     8161 gctgtctccg gcggaaatga ccatatctcc ggacgtcttt ccgtggcata tcagaaattt
     8221 ggcggctggt ttgacggtaa cggcgatgcc accctgcttg ataacaccca gaccggcctg
     8281 cagcactcca atcggctgga catcatggga accggtacgc tgaacatcga tgaatcccgg
     8341 cagcttcaac tgataacgca gtactataaa agtcaggggg acgacaatta cgggcttaat
     8401 ctcgggaaag gcttttccgc catcagcggg agcagcacac catacgtcag taaggggctg
     8461 aattctgacc gcattcccgg cactgagcgg catttgatca gcctgcagta ctctgacagt
     8521 gatttcctga gacaggaact ggtcggtcag gtttactacc gcgatgagtc gttgcggttc
     8581 tacccgttcc cgacggtaaa tgcgaataaa caggcgacgg ctttctcctc gtcacagcag
     8641 gataccgacc agtacggcat gaaactgact ctgaacagcc aacttatgga cggctggcaa
     8701 atcacctggg ggctggatgc tgagcatgag cgctttacct ccaaccagat gttcttcgat
     8761 ctggctcagg caagtgcttc cggagggctg aacaaccata agatttacac caccgggcgc
     8821 tatccgtcat atgacatcac caatctggcg gccttcctgc aatccagcta tgacattaat
     8881 gatattttta ccgtcagcgg tggcgtacgc tatcagtata ctgagaacag ggtagatgat
     8941 tttatcgact acacgcagca acagaagatt gctgccggga aggcgatatc tgccgacgcc
     9001 attcctggtg gttcggtaga ttacgataac tttctgttca atgctggtct gctgatgcac
     9061 atcaccgaac gtcagcaggc atggttcaat ttttcccagg gggtggcatt gccggatccg
     9121 gggaaatatt atggtcgcgg catctatggt gcagcagtga acggccatct tcccctgaca
     9181 aagagcgtga acgtcagcga cagtaagctg gaaggcgtga aagtcgattc ttatgaactg
     9241 ggctggcgct ttaccggtga caacctgcgg actcaaatcg cagcatatta ctcgctttcc
     9301 aataagagcg tggaaaggaa taaagatctg accatcagtg tgaaggacga caggcgccgt
     9361 atttacggcg tggaaggtgc ggtggactac ctgatcccgg atactgactg gagtaccggt
     9421 gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg gtcaatggca aaaatatgac
     9481 gtgaaggaat caagtccatc gaaagcgaca gcttacatta gctgggcgcc ggaaccgtgg
     9541 agtctgcgtg tgcagagcac cacttctttc gacgtaagcg atgcagaggg taacgatatt
     9601 aatggttaca ctaccgtcga ttttatcagt agttggctgc ttccggtggg aacactcagc
     9661 ttcagcgttg agaacctctt cgaccgtgac tataccactg tctggggaca gcgtgcacct
     9721 ctgtactaca gcccgggtta cggccctgct tcactgtacg actacaaagg ccggggccga
     9781 acctttggtc tgaactactc agtgctgttc tgacggggat tcttttgcaa caagggtatg
     9841 ctgtaaacaa catggtcctt ggtggccatg ttgtttacag ccagtataat atacagcctt
     9901 aacaagatat tccgtctccg gaatggactg ttcaccactg aataacaata tgtctgtttt
     9961 atggatcttt gactgaaatt tatcgcatat cctcatttca ttgtccgtct gaaaactaac
    10021 cttacaa
//
LOCUS       DAZFWP020000170        10023 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFWP020000170 DAZFWP020000000
VERSION     DAZFWP020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.170"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            164..316
                     /gene="hokA"
                     /locus_tag="ACHK0Z_RS20845"
                     /old_locus_tag="pgaptmp_004169"
     CDS             164..316
                     /gene="hokA"
                     /locus_tag="ACHK0Z_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU4504093.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(305..364)
                     /locus_tag="ACHK0Z_RS20850"
                     /old_locus_tag="pgaptmp_004170"
     CDS             complement(305..364)
                     /locus_tag="ACHK0Z_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504094.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(504..716)
                     /gene="cspA"
                     /locus_tag="ACHK0Z_RS20855"
                     /old_locus_tag="pgaptmp_004171"
     CDS             complement(504..716)
                     /gene="cspA"
                     /locus_tag="ACHK0Z_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU4504095.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(997..1287)
                     /gene="yiaG"
                     /locus_tag="ACHK0Z_RS20860"
                     /old_locus_tag="pgaptmp_004172"
     CDS             complement(997..1287)
                     /gene="yiaG"
                     /locus_tag="ACHK0Z_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU4504096.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1721..2431
                     /gene="yiaF"
                     /locus_tag="ACHK0Z_RS20865"
                     /old_locus_tag="pgaptmp_004173"
     CDS             1721..2431
                     /gene="yiaF"
                     /locus_tag="ACHK0Z_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU4504097.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2481..3455)
                     /gene="ghrB"
                     /locus_tag="ACHK0Z_RS20870"
                     /old_locus_tag="pgaptmp_004174"
     CDS             complement(2481..3455)
                     /gene="ghrB"
                     /locus_tag="ACHK0Z_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU4504098.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3559..4218)
                     /gene="yiaD"
                     /locus_tag="ACHK0Z_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             complement(3559..4218)
                     /gene="yiaD"
                     /locus_tag="ACHK0Z_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU4504099.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4371..6704
                     /gene="bisC"
                     /locus_tag="ACHK0Z_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             4371..6704
                     /gene="bisC"
                     /locus_tag="ACHK0Z_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU4504100.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6673..7113)
                     /gene="yiaC"
                     /locus_tag="ACHK0Z_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             complement(6673..7113)
                     /gene="yiaC"
                     /locus_tag="ACHK0Z_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4504101.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7110..7673)
                     /gene="tag"
                     /locus_tag="ACHK0Z_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             complement(7110..7673)
                     /gene="tag"
                     /locus_tag="ACHK0Z_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU4504102.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7831..8529
                     /gene="yhjY"
                     /locus_tag="ACHK0Z_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             7831..8529
                     /gene="yhjY"
                     /locus_tag="ACHK0Z_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4504103.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8758..9960
                     /gene="yhjX"
                     /locus_tag="ACHK0Z_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             8758..9960
                     /gene="yhjX"
                     /locus_tag="ACHK0Z_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4504104.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2388 a         2437 c         2586 g         2612 t
ORIGIN      
        1 gctagaatag ccacgggtgc ttgagactgt ttgtctcagg tattcaccga aaggcagaca
       61 gagaaaagcc ccacctgact ataaatcaaa gtgaggctac cctatgcctg aacaccataa
      121 ggttagcctc ttactcgttg gaaatcaaca cagggggctg ggaatgccgc agaaatatag
      181 attactttct ttaatagtga tttgtttcac gcttttattt ttcacctgga tgataagaga
      241 ttcactgtgt gaattgcata ttaaacaggg gagttatgag ctggcggcat ttttagcctg
      301 caatttaaaa gagtaagagt cttcggcggg aagttattcc cgcctttctt acggcgttgc
      361 gcattctcat tgcacccaaa tttatttttc acaaaaataa tactagattt tattacgcga
      421 tcgattattt atttcctgaa aaacaaataa aaaaatcccc gccaaatggc agggatctta
      481 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      541 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      601 ttctggatag cagagaagtg tacgaacacg tctttagagc catcgtcagg agtgatgaag
      661 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      721 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      781 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      841 actacactgc tttaatggtc tgtacgtcaa accgttgatg tacattaagc cacgcattgg
      901 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      961 atcaggaccg taaacagcaa taaagtggat aaaagtctat tccatcaact gcttacttaa
     1021 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1081 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1141 gatttttaac cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg
     1201 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1261 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1321 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1381 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1441 cgatctgata aatgccgata tatctcttac cactcctgtt ttgtaaagat ttcgaaaatc
     1501 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt
     1561 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1621 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1681 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1741 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1801 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg
     1861 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1921 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1981 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     2041 aagcggtccg ctgcgtgaaa tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2101 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc
     2161 ggtctttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2221 gattccggct gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2281 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2341 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2401 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2461 ttacttcacg cagtcgcggc ttagtccgcg acgtgcggat tcacacagtt cttctcaacc
     2521 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2581 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2641 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2701 ttctgcaatg cagcaatcag tgcattttca tcaaccaccg ggccacgtcc ggcattaatg
     2761 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2821 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2881 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2941 atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg ccataccgat ccgtcccatc
     3001 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3061 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3121 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3181 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3241 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatcaac attctcgttt
     3301 gaacccagta aaccttcagc ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg
     3361 ctgaggtttg ccacctggtg aacggtgaaa tgctcttgca ggcgttgcag taaatcatca
     3421 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3481 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3541 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3601 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3661 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgttg
     3721 ctgggagaga cgcatgttca ggtcgtgacc acccgtgctg tcggtataac caatcacgtt
     3781 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3841 cgcaggtttc agggtcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3901 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3961 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     4021 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4081 gcccacgaga gagcccagac ctgcgccgat agcagattta cctgcttcgc gttcgccggt
     4141 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag
     4201 ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc
     4261 gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg
     4321 taagtaatat ttaactgctc aatacatcta acctttcagg agttttcggt ttggccaact
     4381 catcctcacg atattccgtt ctgactgccg cccattgggg gcccatgctg gttgaaaccg
     4441 acggcgaaac cgtgtttagc tcgcatggcg cgttagccac aggaatggaa aactccttgc
     4501 agagcgcggt tcgcgaccag gttcacagca atacacgggt acgatttcca atggtgcgca
     4561 aaggctttct tgcgtcaccg gaaaatccgc aaggcattcg tgggcaggat gaatttgttc
     4621 gcgtgagttg ggatgaggcg ctggatctta ttcaccaaca acataaacgc attcgtgagg
     4681 cttatggtcc ggcatcgatt tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc
     4741 ataaggcctc gacattatta caacgctata tggcgctggc aggcggttat accgggcatc
     4801 tgggggatta ttcgaccggc gcggcacagg cgatcatgcc gtatgtcgtg ggtggtagcg
     4861 aagtttatca acagcaaacc agttggccgc tggtgctgga acatagcgat gtcgtggtgt
     4921 tgtggagtgc taacccactc aatacgctga aaattgcgtg gaatgcatct gatgagcagg
     4981 ggctttctta cttttccgca ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa
     5041 tgcgatcgga aaccgtcgat ttctttggcg ataaaatgga gtgggtggca ccgcacatgg
     5101 gcaccgatgt tgcgctgatg ctggggatcg cccatacgct ggtggaaaat ggttggcacg
     5161 acgaagcgtt tctgacgcgt tgcaccacag gttatgccgt cttcgcctct tatttgctgg
     5221 gcgagagtga cggaatagcg aaaaacgccg aatgggcggc agagatttgt ggggttaacg
     5281 cagcgaaaat ccgcgagctg gcggctcttt tccaccaaaa cacgaccatg ctgatggctg
     5341 gctggggaat gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg
     5401 cggcaatgtt ggggcaaatc ggcacacccg gcggcggttt tggtctttct tatcattttg
     5461 ccaatggtgg taaccccacg cggcgttctg cggtgctctc ttccatgcag ggcagcttgc
     5521 cgggtggctg cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa gcactggaaa
     5581 accctggtgg cgcatatcaa cacaacggta tgaaccgaca tttcccggat attcgtttta
     5641 tctggtgggc gggcggtgcc aactttactc atcatcagga taccaatcgt ctgatccgtg
     5701 cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac
     5761 acgcggatat cgttctgcct gcgactacct cttttgagcg taatgatctc accatgaccg
     5821 gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag
     5881 cgcgtaatga ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag ggcggctatg
     5941 cgcggtttac ggaaggaaaa agtcagctgc aatggctgga aacgttttat aacgttgccc
     6001 ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc tggcaagcca
     6061 accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt
     6121 tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt gaaatcttct
     6181 cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg tggctggagc
     6241 cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct gcccatccgg
     6301 cgcaccgcct gcacagccag ctgaattaca gttctctgcg cgaattgtac gcggtagcaa
     6361 accgtgagcc tgtcaccatt catcctgacg atgctcaggc gcgcggcata accgaagggg
     6421 atatggtgcg ggtgtggaac tcccgtgggc agatccttgc cggagcggtc attagcgagg
     6481 gaattaaacc tggcgtgatt tgcattcacg aaggggcatg gccggatctg gatttaaccg
     6541 ctgacggtat ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc cccagctcgc
     6601 ggctggggaa tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc
     6661 cggaactgac acttacagcg tttgaaccac cggccagctc ataatccagg tgggtagttg
     6721 ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat
     6781 cgccggttga tttttttgat aaacctccag catcagatgg ggatagcgct gctgcacata
     6841 ctgcatcagc gccttaccaa taccgcgtct gacggccttc ggtgcgacaa acattgctgc
     6901 cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtcct cttcccagac
     6961 ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt cacgccagta
     7021 attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt cgaggatcgc
     7081 ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccggata gcaacagcag
     7141 ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca gattgtggtg
     7201 ccgacaaact taaaaccacg ttttttcagt gccttagata gggcgtcgga ggcggacgta
     7261 gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg atttacaaac
     7321 gaccagacaa agtcggcaaa cggttcgccg ttctgttcca tttgcaggta cgcccgcgca
     7381 ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc ctgtaccagt
     7441 ctttcgacat cctcttcctg cattgctgcg accttcaccg gatcgaactg atgaaagcag
     7501 gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc ctgctgccct
     7561 tcaaggcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc ccattcatta
     7621 tcatggtagg caatataaag cgggtcctga ctcacccagc cgcaacgttc catactttcc
     7681 ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag
     7741 tcaatattta atacagggct actatgcccg atatccttca tgcaatgaca ataatattgc
     7801 cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg
     7861 cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac agcttacgcc
     7921 tggcaacaag aatatatcgt tgatacgcaa cccggacatt ccacagagcg ttacacctgg
     7981 gatagtgatc atcaacctga ttacaacgat attttgtcgc aacgtattca aagtagccaa
     8041 agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt gaccagcagt
     8101 atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc ggtcgcggca
     8161 ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag tactaccacg
     8221 ctgaccgatc cgttatggca tgccagcgtg agtagcttag gctggcgtgt tgactcccgg
     8281 cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg cgagaatatc
     8341 tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg caactggctg
     8401 gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta tgccgcgcta
     8461 actcaggcag aaaataccac taataatagc gactatctgt atacgatggg ggttagcgcc
     8521 agattttaac gtaacagtca caattgaaac cattgaataa caacagttgt ggcgatagtg
     8581 ggtgctaact taccaaataa taaatttggt gaataattgt cgcgtcattc attcctgaac
     8641 taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccagg cataaaatgc
     8701 acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa tactgccatg
     8761 acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtactat cattacccag
     8821 tttgcactgg ggtcggttta tacctggagc ctgtttaatg gcgcgctttc tgccaagctg
     8881 gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg gctggcaatt
     8941 tcgtcttctg ttgcgggcaa attgcaggaa cgttttggcg ttaaacgcgt caccatggct
     9001 tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attccaacaa cctgatgatg
     9061 ctgtggttaa gcgccggtgt gctggtcggt ctagcggatg gcgcgggtta cctgctgacg
     9121 ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatatccgc gttcgctatc
     9181 ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcagct gctggaaacg
     9241 gtgggtctgg aaaaaacctt tgtgatttgg ggagcgattg cgctggtgat gattgttttc
     9301 ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa tggcgtagtg
     9361 gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat gttagcggta
     9421 atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa agatatcgcc
     9481 caaagtctgg cacaccttga tgtggtttcc gcagccaatg cagtaacggt tatttccatc
     9541 gctaaccttt caggtcgtct ggtgctgggc attctgtctg acaaaattgc ccgtatccgt
     9601 gttatcacca ttggtcaggt gatttcgctg gtgggtatgg cggccctgct gtttgcacca
     9661 ttaaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt tggcggcact
     9721 attaccgtct ttccatcact ggtcagtgag ttctttggcc tcaataacct ggcgaaaaac
     9781 tacggtgtga tttacctcgg tttcggtatc ggtagcattt gtggttcgat tatcgcctca
     9841 ctgtttggcg gcttctatgt gaccttctac gtgattttcg ccctgctgat tctgtcattg
     9901 gcgctttcta cgacaattcg tcagccagag cagaaagtat tgcgtgaggc gcatatttag
     9961 aaatatgttg ctactgaaat aaatgcgagg agggagactt caaagtctcc ctttttttat
    10021 gga
//
LOCUS       DAZFWP020000171         9916 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFWP020000171 DAZFWP020000000
VERSION     DAZFWP020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.171"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..600)
                     /locus_tag="ACHK0Z_RS20905"
                     /old_locus_tag="pgaptmp_004181"
     CDS             complement(<1..600)
                     /locus_tag="ACHK0Z_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504105.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQK"
     gene            complement(621..890)
                     /locus_tag="ACHK0Z_RS20910"
                     /old_locus_tag="pgaptmp_004182"
     CDS             complement(621..890)
                     /locus_tag="ACHK0Z_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4504106.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(969..1607)
                     /locus_tag="ACHK0Z_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             complement(969..1607)
                     /locus_tag="ACHK0Z_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGU4504107.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(1592..2823)
                     /locus_tag="ACHK0Z_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /pseudo
     CDS             complement(1592..2823)
                     /locus_tag="ACHK0Z_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(3324..6005)
                     /locus_tag="ACHK0Z_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             complement(3324..6005)
                     /locus_tag="ACHK0Z_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU4504108.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6246..7292)
                     /locus_tag="ACHK0Z_RS20930"
                     /old_locus_tag="pgaptmp_004186"
     CDS             complement(6246..7292)
                     /locus_tag="ACHK0Z_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU4504109.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(7396..7545)
                     /locus_tag="ACHK0Z_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             complement(7396..7545)
                     /locus_tag="ACHK0Z_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504110.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            7967..8158
                     /locus_tag="ACHK0Z_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             7967..8158
                     /locus_tag="ACHK0Z_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4504111.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(8211..8444)
                     /locus_tag="ACHK0Z_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             complement(8211..8444)
                     /locus_tag="ACHK0Z_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504112.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(8540..9163)
                     /locus_tag="ACHK0Z_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             complement(8540..9163)
                     /locus_tag="ACHK0Z_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4504113.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9252..9761)
                     /locus_tag="ACHK0Z_RS20955"
                     /old_locus_tag="pgaptmp_004191"
     CDS             complement(9252..9761)
                     /locus_tag="ACHK0Z_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504114.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT         2727 a         2540 c         2022 g         2627 t
ORIGIN      
        1 cttttgttca tcatcataca gagattgaat ccggaattta atctcttctg caaccagtct
       61 tgcatcgggc tcataaaata tttttgttat tcgctgtttc tgatgttcag gctggtcttc
      121 catatttcgt aaaaccagat gaaataatat atcataaagt gcgcaatgcc atgaataacc
      181 atcattggtt aaaataagct cccaaacccg ctcctgctcg gcaataagct gcagaacaaa
      241 ggactcaacc tgttctgttt gttcgggggc aacacggtat agcggagcaa tcacagcaaa
      301 agatagttca tttaaccagg attccgcttc cttctcgctc ctgaacggtg gacagttttg
      361 tattcttcgt tcgtattcac gggtaatctg ctccagaata tcctccggcg gtgtcagcca
      421 cttattcctg agcaatacac tggttgatgg atgacagctg gctatttcag caataatttc
      481 acgtaatgtc tccgggctga acttcttcag ctgattgtag tgctcccggg taaaaccatg
      541 ttcatcgcat aaaagttgcc atgtcattga tatactgttt gccattcctt gcttcatcat
      601 taatacctgt tacctgaata tcaacagacc ggtgtacctt ttctgaatac ctccccggac
      661 ggagtggcaa tccccattcg cctcagcgtg gcatctgcag ctttcgccac ctcgctgata
      721 tccagcgttt caggtagcgg cggggacgat atctgcgcca gacaataatt ccggcgcaac
      781 aacaaccact cccgttgtgc ttctgtacat tcaacatttc ccataacaaa aagccagcgg
      841 tcagaagctc tgcgccagca accagcagcc tccagtttct gcgcaaccat gtcttccaca
      901 acttccgggg tactcttttt cctgctcaaa acattaactc actgaaataa tatacatatc
      961 tgacggtctc agcgaaccgt ccatgccggg ctgaaatcct cttcctgaaa cagctccgtc
     1021 agcccgctaa tctgtttcaa tctgagaact tcatcctggt ccatacccag ctccgtacca
     1081 atgcgttcat tcgtccagcc caggcgcgac aggtccctga caatatcaga cattgaggtt
     1141 atctgatgtt tgcccctggc ccggttatgt cggatggtcg cagcaatacg tgaggcctgt
     1201 ccctttctct ccggattaat acagaccacc ggcagccagc ccttcagacg ttttcccgta
     1261 cctgcttccc tgcccagtac ctggcgatga aaaccatcca ccaccaagta atggttcttt
     1321 tcctcagaca ctaccaccgg ttgtgtgaaa ccatctttct ccagtgactg cttcagcagt
     1381 ttcttctcac ccggcgccat gacattagga ttgtaatcat tggccaccac ctcatctgct
     1441 tttacccaca gcacacagtc cacaggctcc tgtgcaaaag gactgacctc atgaagcacc
     1501 ctgcgcaggt gattaagggc ctgtatggcc tcttcttctg gtcgttcctg gctgaagtac
     1561 tgacgaatca tgttgccaag tatctccggt gtcacagaat cccccattcc ttcctccttt
     1621 ccttcatacg ctgcaggtaa cgttcatagt gccggggttt gttagggcta aaggagaggg
     1681 tccggcacca gaaatcattc tttatgagag tcttacagat acgtcgccag gacggaatat
     1741 cccggctccc caggtcattc tcctgttcat ccggaatgtc atccgggaag ccccgcgtct
     1801 gataccaacg caggtagaca gcaattttat tacggtaatg ttctgctgtt ctttccggca
     1861 tcacatccag caggaacatc gcatagctgc gccaggtatg atgaggcggc ttcgtgatac
     1921 gcttacacag ggcaaaatag gcaccactct cattggcata aagcgccccg ctggcagccc
     1981 ctgacacccg ttcacacatc ctggcccagg tttccggctc cagaacatgg taaagccaca
     2041 gtcccttacg ctgttcagga ccaaaaggtt cacagacacg catgttgcgt aacggcacgc
     2101 cggcacggta catcaggtca tacagaggat tgtaaatggc acaggttctg gcgttatata
     2161 tccagatatc gcgggccttc cagtcataca acggatacat ggtgtaataa aacccttcag
     2221 gcgatgccgt ggtccagggc ttatcatcgg catatctcag tttgcgctga gataccagtc
     2281 ccataaagcg attgagcgat tcatcagcac ggacaccagt cagtattgcc actccacacc
     2341 ggttaccggc aaaccaggaa gaaaatgccg gaacaaattc ttcaaacgtc atggcatacc
     2401 ggtaaaaggg aaaatacgtc atgtctgtaa tggcctcttc cggtggctgg cgaacccagg
     2461 tcactcctgg ctcccagcat atccactccg gctgaaactg cgagacgccg tttaccgtgg
     2521 tcagggaagt gccacccagt aaaaggtttc cgtcacatca tggtacattt cccgcatctt
     2581 ctgaatatgt tcaatagtgc accgatactg ggcctcccag tcaatgaaca gaacagagaa
     2641 atgacgtttt ctcctgcggg ccacctctgc cacaagatgg aaaagcacag tggaatcctt
     2701 tccacctgaa aaagacaggc ataccgacgg aaatgtttca aaaatccact caatgcgggc
     2761 gattgcggca cgcaaaacat tttctccggt aaggattttt tcggttaaaa gcgtatcact
     2821 cattttcctg gctttatgtc gaataattct gtttatgcat tcagacaaac ttcattaata
     2881 actcaccgct ttggggataa ataataacaa taaaactctt cagatattaa tctggttgtt
     2941 ggatttgtta tcacatcctt tatccacctg cttcttactg gtaacacatc tggaaaatcc
     3001 atgcattttc aacatatacc atcctttcaa cttgtaaata aggaaatatg atgcagatgg
     3061 tgaacaattc aaatatcatt ttgcaggaag tattcactgt aatagaatta ccccctgata
     3121 aagtttattc actaaggaat tattcaacag aaccattccc tgttagcttt gagcaatagc
     3181 acttattttt atatgtatca ggaatacgta cgttgatgta accaaaacaa agaagcctgt
     3241 gtccatgggg ttcgtacaat tgtgtgtggc acatgaccaa atcaggtttg agacctcccg
     3301 ctgcaatatt ttttattcag actttaacct gtcaggcctg acagaaacgc tcccaacttc
     3361 ccttctatat cactgtctat agtacgtaat cttagcaatg gcaggccgca tttcttcagt
     3421 atactgtttt taagttcatc acgttcagcc tgaacactgg tcagatgata acctccatcc
     3481 acttcaataa cccctaatgg agtcttcccg accttatagt aaacaacaaa gtcacaactg
     3541 gcccggttac gcatgaataa ctgttcacgg tgtgtaaaat ccccccgctc cagcaacacc
     3601 aactgattca gcgctatttg tgaatgaaac atcatactcc gataactatc ctgcgacagg
     3661 atatcccgca acaggcaggc cacaatctgt tctgacttaa agtgtgaatc atttgaattc
     3721 aatctactat tcagtcgttc cagtgatttg tcatactcac tgtacagcaa atcaaatgct
     3781 gatatgaccg ggctctcgaa aatctctccg tcatcagcgt agtatttgat gtaacgaatc
     3841 agcgccgcaa tatgtccagc gtggcgttca aatacatcat taccagtcac cagcgtaaat
     3901 ttatttcggg cccttgaaac agcgacattc accagttcag gattatcaac aaacgcgatg
     3961 ttccgactgt gctggctgct gcgcttctta tccagcacag tggaaaacac aatttcatcg
     4021 cactcccgtc cctgaaattt atgcaccgtc gatttcacaa aatcagcagg cagcactgtt
     4081 tcagccagat taacctgtgc attatatggg gcgatatatc ccctgctact ttcctcatcc
     4141 cagtaatgtc cttccagaga ttcaagctcc ctgagatttg aaaaattacg ggtatggttt
     4201 ccttttgccg tgatgatcag agataatgag gcttcacctg aatcaagcgt tagcggtatc
     4261 agagcattat cataaaactg cttattacaa aattgaatga ttttaggatg acagcgataa
     4321 tgctctttaa gaagagttac tggcaccata ttcctgaaca acatacaaac cgaatcaagt
     4381 agactatatt tctcacaatt atagtattct gccggtggag atgggctgtt cgggagtaat
     4441 acaggaacat gtggaagttg ctttcgatcc cctacaacga ttacattacg ggcacatccc
     4501 agtcccagaa tgcccggaac aatatcctgc tgtgaggctt catcaataat cacataatca
     4561 agaagagccc ccttaccgat agagttaata atagaatgcg tgctgctacc aataaccgga
     4621 aaacgcttta taaaacggtt aaattcatcc ctgtatgtct ctgccgagaa tgaatttctt
     4681 gttgaaatat ttcttcgaag atgttgtttt aaataaagca cagaccatga tgtcaggcgc
     4741 tccagtaaaa ttttaaaatc agattccttt aatacttcct catattcatt cagagaaact
     4801 tgtttttccc ggatgaattt ttcataataa gaaagctgaa gagaagtaaa tagtgccagc
     4861 cttctctccg gaatcaccaa tggtttgact ttcagtatcc tgaagtttaa caacaaatca
     4921 attctgtttc tgaatccaat ccgcctgtca gaaaggtaat gaatataagc catcaggtca
     4981 gttgtttttc gtgaggaaag tcggtatttg tgagaaaaaa gctcctccgg tcgaagattc
     5041 tgactctgcc attgctgcag gtatttatac tcaatattca gctcattaat ttctgctttt
     5101 aacgaagcga cctggttaat ggcattcagg tgttttttca cttgttgaag tacatcatca
     5161 atcgcatttg cagacggtga gtcaggtaag acttcctcag aacgcgaaat cgacgttgaa
     5221 aaaaactgtt gcctgttctc cgtactcccc agtcgtgcca ccacatatcc cagttgctgt
     5281 ttgtccatct tttcataaac attactgaca gctgagttat tattggacaa aattgccact
     5341 gttttattct gaattaatat attagcaaca atattgagta tagtctgtgt tttacctgtt
     5401 ccaggtggtc cttcaataac tgaaacctgt gaggaaaatg cccgttcgac agccagcaac
     5461 tgactttcat taagaccaaa cggaaaaatc aaaccccatg gcgaatcacg ttttttactc
     5521 tgtcctgaac agtatgcatg caaggcggta tccggatgag gaactatttt ctttatctgc
     5581 ctaaggatat tctcatcgat atttctgtta ttaccgcggg cataaaaaat acgttcatta
     5641 gccaccctac acaagtaatt aaacacggca tgttctgtaa atgctgaaca ctgttgaaag
     5701 tcaagaccgg tcgttttcat gatgtagggt cgttcaccct cgcgataata aacagcagca
     5761 tatttatcac catatataat aaccagttca gcccgggatg ttacagcgtt ccctttcctt
     5821 tcgaggaaaa catttcgcaa atttacagtt ggcgttatct gacatacaga aagcggacga
     5881 gtatattttc tcccgctgcg aaaatgacag gtcagcataa cctctctgtc gtcatttcca
     5941 gcccacagcg tccagtcggt aattttatga gtcttatttt caccgtcaac acaaatcgtg
     6001 accatattct tactcagaaa ttatcattta acttacggaa aattgttaag gcactcccga
     6061 tattagctcc ggaggatttt ccacaactcc gggactgtta acatatttct gcctgccata
     6121 tatacattga atttagtcat aacagtcaat accagaatta caggaatgcg atgccgatac
     6181 gctggttatt gaatactggt agcccctgag cactaattca tcacgatact ctgtatttac
     6241 tgttttcact gctttttaca accattcccc caggtaaaat gctgctcctc cggtgatggc
     6301 tgtgtcagaa actgccatcc cggaatagcc acgctgaccg ccagcaatgc cgcacagaca
     6361 ccagccacaa acggtttcag aaacgtgggc ggggatttct gaagggaggg tgactgctcc
     6421 gcctgcactt tccccttgcg attatcaagg ctcagtgcat ccagacgcac cgcgccattt
     6481 gccgcaagca gcccttcccg gttatccacg cttcccggca gactcagcga aaaatcccct
     6541 gttcccgtct gaatcagact gcctttacga tttgacaggc tttgtgccga tatctgcagt
     6601 gtgtcggcat ttatcgtacc gccattactg ctgaactgcc cggcagtccg ggcggaaagc
     6661 gtatcggcac tcagttgcgc attctgtgta gacagatttc cactggttgc attcagctca
     6721 gtatgttgtc cctgtatccg gctgttacct aaatccagcc cctgctcaat gtcccgtaaa
     6781 tccagcagag aaccttcgtg tgccgggaat gaggaataca actgaatata gtcatcactg
     6841 tccggtgtca gacgcacatg tcgcacaaca ccgggaataa taaccagccg gaggataccg
     6901 ctttttaaat cctgcgaagg tgccagaaca cgggtggtga cataaccatg atcgaccaga
     6961 cggttctgca atgtactcat cagcagatta attcctttcg cccccaggca atgcccgacc
     7021 gccccgttgg cgattttttg taaaggcagc cagtgtggta acgcatcagc ccccttcagt
     7081 tccgtctgtt taatctgaaa acagggcgtt tcaacaggaa aattaatctt atgggcaaaa
     7141 gagccaggtg cagaaaggcg aacatcaggg gctgaaggcg tcagttgttg ctcaagggct
     7201 tcctgacgct gctgctgaat gataaatgct tcatctccgc tacgccggac atccgctgcc
     7261 tgaagagata aaggcgataa taaagcagac atcagtgtca gtgacagcaa ggacggacag
     7321 cgccggaata agctttgttc ctcagtcctc tcgttcctgc aacataattt tccagtcaaa
     7381 cgaaaaacct tttattcatg tcataccaac aatcaccagc ctcctgacga tacctcccac
     7441 catgttttgc gatggcacgc taccgccctt ccggtattta cccgcatcaa ccggtaccag
     7501 aatgctgagt aacaaaccag ctagaattga cttaattttg atcattatag aataatgatc
     7561 tgaactacag caacatgaca gatttatcga tcgatataac acaaatgaat agttctttat
     7621 tctgacaaat atcgcaaaaa cagcacatta caaaatttat aaccataatt actggaaaag
     7681 tgcagaaaat tattccactg aatgctttta atacagttgg ttagcttaaa aaatagcaat
     7741 ttttgatttc agtcattgaa agttgcaatg tttgacgatt caacagcatt taagtgagtt
     7801 tcaggatatg ggaaggacat gaaaggagga gcgcaataaa atacacagga agaaggggaa
     7861 aatgtttcgt aaaaacagaa aattaacagc gcacatcagg aaacgcagtc tcctgattca
     7921 gtgctaccac atgaaaattt agttaactgt taccgtacta aaatttatgt cagaagatgt
     7981 tccattaccg aaagtcaatc agcgatacaa agacgatcat ggtgcgcttg tcaccctgac
     8041 cagtgttgag gaaacccggg tggtgtttat gcaggacggc tatccgcatc cctgcatgcg
     8101 accgatgtac aactttctgg gaaaattcaa acccgaaccc cgggaagaaa cagaataaaa
     8161 cgaaatccct gccggcaatc acactacctg atagcagcat aatccactta ttaattctca
     8221 tccccggaga caagcccaga aaaccactcc gggtgttcac tgtatgccgt gttcagcgca
     8281 tccctggaaa cgtttatata aaagctgtcc tgcaatttgc gggtaacaac ttctgccagg
     8341 cgattccggt tatccaccag tccggcataa cctctctctg ccagccactc attcagttca
     8401 tgttccagag acagattgat aatatcataa tcggaccttg ccattcccgt ttccttagca
     8461 catgttttca ggcacgggga taaagtataa agaatgtcat actgctgctg ccagagcaat
     8521 attccccctg atggttgtat cagagatggt atgccggtac tttgcgggtt gttcccgtca
     8581 ggttatcgtg cacctccacg gtcgtggtca ccaccggcat tccggccttt ctcagcctca
     8641 gaacatcagc cgcaatacgc tgactgccaa accagaacag gccgtccagt gcagtcacca
     8701 gtaaacccgc ctccagtgca tacttcagcc gttcacgggg cgctttcact tcccgggcaa
     8761 cctgctgata tggcaatgat gtgttttcat tcccaaccac gcgacgaata cggtgagaca
     8821 gatgataccg gtatgtatcc ggcacaccag aaaggctggc cttcaggctg tacacgcagc
     8881 caaaccgttt atcgttaaac accacatttt tctggctgat gccccattct tcacgcagcg
     8941 cggcaatcaa ttgcggtgta cgggtaagca gcaagcgaaa aggcagttca aaactggtga
     9001 cgtaatccac attcagcagt gcaatacgca accgttcttc tggcccggcc tctgtctgcc
     9061 ggcactcctc cagaacatcc tgccactgcc ggcgaagacg gggggattcg ttccgttctg
     9121 taaaacagta tttatccgcc agatagtcaa ttcgtgtacg catactgaaa ggaattccgt
     9181 ataaagattc agcagacaaa actttatcag ccagtaaaaa ttaacggaag cattgatatt
     9241 cctcccgaca atcaccggat gattgttgca ataacctgcg acataaagga ctgaacagcc
     9301 gtttttaacg ccacgtattg ccctgatgaa ccaggccggg ccacccgaaa acatctttcc
     9361 acccggcatt gtccgccagc acttatcacc gaccaggtct gttgcagtaa atccaccagt
     9421 tgcgcacatt cttcatcagc catgacgccg actcgcggat ggcaaatact cgtctggctg
     9481 agcagcaaca gacatcgtat ctgtgctcct tcatgttgtt cccgcaacca gcgtaatacc
     9541 accggcgctg atgggctgac cgtgtgatta tgctccgtaa tacgttcgtg tacccgtcgg
     9601 tgaaaggaac tacaaaatgt ctgaatctgt tgaccataga ttccttctgc cgaatagagc
     9661 gtgaagtgaa atgccaacag atttggccag tggtccaccg cctgctgaac aaaacgccgg
     9721 atttcccccg gctctgaaat taaggcttcg gttatttgca cgaaatgttc cttttcaacc
     9781 cctgttgaat tttgtcaagc cggtgcagac acagtaaagc gcgcatcaac acctgaaagg
     9841 tacgatacaa acacaagtta caaacaataa gaacggaagg acaattaatc agtgaggaat
     9901 aataactaaa ttaacc
//
LOCUS       DAZFWP020000172         9909 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFWP020000172 DAZFWP020000000
VERSION     DAZFWP020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.172"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..183)
                     /locus_tag="ACHK0Z_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             complement(<1..183)
                     /locus_tag="ACHK0Z_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4504115.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYR"
     gene            complement(408..962)
                     /locus_tag="ACHK0Z_RS20965"
                     /old_locus_tag="pgaptmp_004193"
     CDS             complement(408..962)
                     /locus_tag="ACHK0Z_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4504116.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(971..1330)
                     /locus_tag="ACHK0Z_RS20970"
                     /old_locus_tag="pgaptmp_004194"
     CDS             complement(971..1330)
                     /locus_tag="ACHK0Z_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4504117.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVSGGRLGVKIYKIESE"
     gene            complement(1343..2392)
                     /locus_tag="ACHK0Z_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             complement(1343..2392)
                     /locus_tag="ACHK0Z_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4504118.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPPHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(2394..2666)
                     /locus_tag="ACHK0Z_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             complement(2394..2666)
                     /locus_tag="ACHK0Z_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504119.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            complement(2788..3132)
                     /locus_tag="ACHK0Z_RS20985"
                     /old_locus_tag="pgaptmp_004197"
     CDS             complement(2788..3132)
                     /locus_tag="ACHK0Z_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGU4504120.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(3252..3407)
                     /locus_tag="ACHK0Z_RS20990"
                     /old_locus_tag="pgaptmp_004198"
     CDS             complement(3252..3407)
                     /locus_tag="ACHK0Z_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4504121.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(3698..4255)
                     /locus_tag="ACHK0Z_RS20995"
                     /old_locus_tag="pgaptmp_004199"
     CDS             complement(3698..4255)
                     /locus_tag="ACHK0Z_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4504122.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            complement(4257..4475)
                     /locus_tag="ACHK0Z_RS21000"
                     /old_locus_tag="pgaptmp_004200"
     CDS             complement(4257..4475)
                     /locus_tag="ACHK0Z_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4504123.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            complement(4603..4914)
                     /locus_tag="ACHK0Z_RS21005"
                     /old_locus_tag="pgaptmp_004201"
     CDS             complement(4603..4914)
                     /locus_tag="ACHK0Z_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504124.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(4907..5134)
                     /locus_tag="ACHK0Z_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             complement(4907..5134)
                     /locus_tag="ACHK0Z_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504125.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            complement(5131..5412)
                     /locus_tag="ACHK0Z_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             complement(5131..5412)
                     /locus_tag="ACHK0Z_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4504126.1"
                     /translation="MILRQCAGTRKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            complement(5445..6161)
                     /locus_tag="ACHK0Z_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             complement(5445..6161)
                     /locus_tag="ACHK0Z_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4504127.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            complement(6183..6929)
                     /locus_tag="ACHK0Z_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             complement(6183..6929)
                     /locus_tag="ACHK0Z_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4504128.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLVEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            complement(6936..8018)
                     /locus_tag="ACHK0Z_RS21030"
                     /old_locus_tag="pgaptmp_004206"
     CDS             complement(6936..8018)
                     /locus_tag="ACHK0Z_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HGU4504129.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKDTDQ
                     EDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARPEQ
                     SHQLQQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAF
                     SAWKARLREGVPPEAILDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLP
                     VSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            complement(8090..8515)
                     /locus_tag="ACHK0Z_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             complement(8090..8515)
                     /locus_tag="ACHK0Z_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4504130.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(8499..8741)
                     /locus_tag="ACHK0Z_RS21040"
                     /old_locus_tag="pgaptmp_004208"
     CDS             complement(8499..8741)
                     /locus_tag="ACHK0Z_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4504131.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            9133..9471
                     /locus_tag="ACHK0Z_RS21045"
                     /old_locus_tag="pgaptmp_004209"
     CDS             9133..9471
                     /locus_tag="ACHK0Z_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4504132.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPNRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            9764..>9909
                     /locus_tag="ACHK0Z_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             9764..>9909
                     /locus_tag="ACHK0Z_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4504133.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNS"
BASE COUNT         2316 a         2768 c         2164 g         2661 t
ORIGIN      
        1 tctgtaacaa ccgcactgat agtctgccag acccgccata accagctggg tcagtattaa
       61 ctggcagcgt tcgcgtgaaa ggtaagtatt ctgcgcaatc tccccgactg tcgccggttc
      121 ggtaacgctt aattcattaa acaccactct ggcggtttct gtcatatcct gctgttttag
      181 catgtctttt tcccttttcc ggttaacgtg acataccaat aactcttgtc gaaaaagcca
      241 gcaagttgaa agaccggtat tagcaaccac cagcgcgttt aacgccccgt gccgtttttc
      301 agtcataaaa aaacccgcaa aaagcgggct ctttcaaatg tccatgtctg ctattcgcct
      361 cgcggtacag ctttgcgaag cttaccggaa ttgaagcagt ttttacgtca aaaagcaata
      421 acttttttct ctataccaaa agccataacc attggtttgt acaaaataaa ttctgccacc
      481 tttagccaat gctcaatgcg tctttcacag gttcttaaac tccattccgg atgtgcatca
      541 ttcagcaatt cagccatttt gcgcttagtc atcccccgcc ccacataacg ctgactcaga
      601 acattgagca gcccgggata acctgccagt acttcaccaa taaccctgtc gattattaac
      661 gcctctgaat cggtacaatg tgccagccag cttttttgat tgccgttgat catatcccgc
      721 aaaaaagcct caagttcagg tttgtccaga cccgcttttt tcatcctccg gagcgcctcg
      781 ttaattgccg tttttgtcag ctttttagag gtcagtaatt ggttgaacat atttcccgtc
      841 ttaccgtcgc caatatacga ccaacgcccc cacatacgca gtttcccctg gatccagaca
      901 ctttccagcg ttttcaggcg taaatgctca ccgcttttgc ctgtaatttc cgggtatatc
      961 atatttatgc tcactcactt tcaattttgt aaatcttcac gcccagccgc ccaccagaaa
     1021 cgagctggcc gcgcacaata ttgatttcat caaactgctc gtcgtctatg agaagtccgg
     1081 catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga cggcgcttat
     1141 ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat ttcagccgct
     1201 gctggcgaac aataagtgcc acatcccggc gataacgctc accggctttt gatacaaaat
     1261 atgtgctgcc acgacgtcgc cagtaggtgt tcaccgtcgg cgggtaaggc aaaacaaact
     1321 ctatacgcat cagtaacctc ttttacccga gcacgccggt tgcaaaggcg tgatcaagaa
     1381 aacgaaaaat taaatcaacc tgggaaccat gcttttcttc gaacgccagc ggatccgcat
     1441 gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtgg gattttgttc
     1501 ccattccgcc ctgaccatga ccaatcagat gatgaggatc gtccgctggc ttaccacaac
     1561 acgcacacgg ctgtgtcttt acccagcgtg tgtatttctc atttacccag cggcgacgtt
     1621 taggtcgttt catgaaggat tccggagact ccggatcaac ggcaatgctg accaccgtct
     1681 tttcctgtgg tgggttctgt tgctggtggg tgtgaggcgg tagcgcaata ttttttgtgc
     1741 gctgcttcag tatgctggtg gcggtctgtt ctcccggtat gatgtcgctt tcacggtata
     1801 cggagcggat tttttccgcc ggtaatccca gagagcgacg cgatactgcc tcaggtaatg
     1861 cgtccaccac ctgattgcag actgcccacc aggataattc agccagcgat aattcccgct
     1921 cctgcgtgcc attcattaca tggcgtatga cgtcaatcat ccaggctgtc agattttgtt
     1981 gagtaagttg ctcaagtgat tcggaggtct ggtcgcgcag ctggttgtcg cagtgccagc
     2041 acaacaccat tgcgccagca ccgtaactgt gaataacggt ttcgctgtga tgataatcgc
     2101 cgtgtggcca ctggcaggat ttcacgtgac gtaataacca gtcagacagt gcaccagcgc
     2161 cgccagctgc acgaatcacc cgctcatcgc tgaaaaatgg cagcagtgat ttatcttccg
     2221 ccagcggctg gcgaacagca ggaaccgctc cagatggcag cgcccgcatg tttttcggtt
     2281 ccggctccac cagcaccctg ccgcgatgaa aaactggcaa tgattcacga ccgggcttaa
     2341 ggaccaccag cccgagttcc ggaaccagaa caggtcgaag taatacccgc acattacctc
     2401 cagacgcgct gacggtagca ggcatgtgtc cgtggcagat gtgcacgaac aggaagatat
     2461 acagaaacgg tccaggtcag acgatcagcg ttcagactcc gctccacacg gacaccgcga
     2521 cgcagatacg cctcttgaag catatctgcc tcatcgatcg tacagaacag atagtgaaac
     2581 cagccatact gaggcgcacg aaaacgcctc ccctgcttaa tttccgggtc ggcttcagaa
     2641 ttgtgggatt ttatgtgttg tgtcatcgga ttctccggtg acagcaggtg tcagttgttc
     2701 aggctgactg cgcgaattgt aaggcaatac gccggaatgt acaaacagaa aacccgtcag
     2761 taagacgggc ttaacaagca ggggcggtta ctttaataat ttcagtgcct ttacatcaac
     2821 ttcaacactg ctcaggtctt tatcaatttc accctcaatt cttactttgt ctttcggaga
     2881 aacattctgc ccggcccata cgctgtcatc aatatccgtg acaattgtcc cgctattgtc
     2941 acgaaactca taacgttcat cacccacttt tttaacgatg ctcccttcaa ggataaccca
     3001 tgcatcatcc ttcagttctt ttgcctgcgc tactgttgaa cgctctgctt ctggcccctg
     3061 gaaaccaccc tgctgtgcaa aagcgccaaa agacacaccg gaaataagtg ctgcaatcaa
     3121 tacctttttc attcatagtc ctctttcaga gatgaacatt caaacagcat tttcagtatg
     3181 gtaaagcgcg ggtgcgttga ggatgcctga cacatcagag gtggcgggag attactcccc
     3241 cgcctggtct cttacttctc agattcgtag tctacgaaga cagcgacctc cgtctgaccg
     3301 gttcggattc gcacctcgca gaggtctttc ctcgttacca gtgccgtcac tatgacggtt
     3361 aaacagatga cgatcagggc gattaacatc gccttttgct gcttcatagc ctgcttctcc
     3421 ttgcctttcg gcacgtaaga ggctaaccta catgtgttca gcatggattg agcctcagat
     3481 taatgttaag cgtcttgcag gacgcgtaat gttaactggg gcttttctct atctgccgtt
     3541 ggtgttcatg cccgaggcag atagcctcaa gcacccgctg caattctact taactatcct
     3601 tttcccgcaa accgttttta tccccagcgg caaatcgaat acaccaccag cgccaccgcc
     3661 atcgcaattc ctaccgttgt gaatgcttca ggccaggtca ttgattcacc tcctgcggcg
     3721 gttctggtag cgacatccag tgggttgctt gcctaagatc attacccgga ctaactgcta
     3781 tacctccgcg ccggaacgtg cctccgagat agcgtgcgga atatattaat ggcccagcct
     3841 cgctatcgat attcatcgaa ataagcacgt tctggctctt ttcaggcatt cgctcagtac
     3901 agcttatcca gccatccaga gttatcggag atctggttga cgtttccgag atttcccgaa
     3961 aattgttggt tgacgaattc ttattttccc gaaagtttcc ggcctgaagc atggcgacgc
     4021 ggcaggcgtt ccagccatca gcatatgttt tagttacacc gtcgagatgg caggtaagca
     4081 aatccatttc atcaggcact accattgcta tcggctctgc ttccagtgat gccagcgcga
     4141 tacgaaacac attagccagc aggctgtctg aagacttgtt atcgtgcgcc gagtcgctca
     4201 ggaagcctgt gatgtatgat ttaatctccg cgcgttctct ggtaatagtg ctcatatcag
     4261 ttttccttat acggattaat tttattgtgc agtgcgctga atgattccca tgtcacatcg
     4321 gtatatagct caataactgg ttcaaatgtc cttccaatta tccagaccag taatagcggg
     4381 gatatcggta tcatcaacac tataaacaga atgaaaaaca gaaactctgt tgttctgctc
     4441 tttcgtgggt aatttttttc taaataatgt ttcatttctt accgcccttt cgggcggcct
     4501 cctgatgttc tgagggtgca ggaatccctc cggttaagga tttaataaaa atcgtttctg
     4561 atttaaatct tcagtattta gttgttagtt ggtttatcgc ctttatgctt cagccttatt
     4621 tcgcaaccag acacaaaccg ggccatcttc cgtatcatga atggaaccaa taaaccagcc
     4681 atcgccctct ggtcgttccg gttcccatgc agaaatatca gcatcacacg catcaaggtc
     4741 agcacatcct tcatctctga agcagaggac gtattgaaga ttattttcct ccatccaggc
     4801 gttaaactct tccgttgaaa tatattcccg accatcacag aatttttcat attcaggatg
     4861 tgtccagtag ccatatccgt cacgaactac cggcatttct ttaattttac tcactgttaa
     4921 cctcctgcaa cgctacacga tacgccttct ttatccacgc cttactgcca tataatttcg
     4981 tcttcataat aaacacacct gcacgactcg ccgatatccc cggacaggtt aacagcacag
     5041 catccaccac acggttatgc ttccgaaact ccattacagt actgctgata accacctgcc
     5101 ccaccgggcc gtaatcctga tacaggattt tcacgcagac accctcctgt cgaaataaac
     5161 gtagttattc actatgcgca gcggcatgcc taattttctg gcaatttccc ttctttgcat
     5221 gcctctctga tgcagttgcc gcgccagctc aatatcacgc tgagaatatt ttgccgacgg
     5281 gtgaaaatca ccacgtaaca tcatgctgat gcccagctcc cgtgccttcg ttctcactgc
     5341 cgcttcagtt cgtccgataa gtgcgccaac gctttttacc ttcctcgttc ccgcacactg
     5401 ccggagtatc atgatttccg cccggcacca cgtcttccac ccactcaccg ctgctgttct
     5461 ctggtggcgg taatatcccg gagaatatcc cggcacttgt tcagctcccg cagcgcggcg
     5521 cagactcgct cccacttctg aacctgacct tttgcccggc gcagctcgcg gttagccaca
     5581 tgcagcgatg gtaaaatcag accatccgga tgctttctgg tgaacgacgg ctgtgactgc
     5641 actgtgaccg ccacactttc cgtttttatt tcttcctgtg tttccgcttc cccgactggt
     5701 aacgcaacac ctgctggctg aggaaaggct ttaccatccg tttccgctat ggatgcggct
     5761 ttcggctctg ccggtaaatc agcgcccggt atgcagtacc gaaatttacc gccctgattc
     5821 acgcgaatca gacgcccttt gctgattgcc atggccagtg atgaattcgc ccggcgggag
     5881 gtaatcccga acatcagtgc cagctcatcc gccgtttgtg ggccatgatg ttcaatcgcc
     5941 tcagtcagca tttgcgctgt cactttcggt accggtgaca ctggttcact ttcaccagcc
     6001 tgaatcagcc accacatcga acccttgtta tccgcttcac cacggcgctt cagtttccac
     6061 agttcgttga cagcatcttc gcggctgatt ccaaggcgcg atgccactac ctgtgaagag
     6121 gcttttttca gtgctttcag tgcgtcaaat acagtttcca ttaaaacgtc ctccaacaaa
     6181 aattacttta caaccctctg attgctgaca tttggacgcc agctatccca gttaaacgtc
     6241 acccaccgac caccgttcat ggtcatgcgg tccataatcc tctcaccaag aagcgtgctc
     6301 attgcggcat gattcaggtt tgttaacatc ccgacactgc acagtgatgc tgtccggcga
     6361 tcaattatct ggtgtaatac cacctgctcg tttttcgtct cccgctgaac gcctatttca
     6421 tccaggacca gcaaatcaac actgcaaagc tcctgtaaaa atttttcccc ggatttgccg
     6481 ttgtcgtagc tgtcatgcaa cacgctcatg acatcagaca cggtgacgat aatcacgctg
     6541 cgccccttcg ccatcagccg gttacccatc gccgctgcaa ggtgattttt cccggtgccg
     6601 gttttaccgc tgaacacaaa attcgtgcac ccggtcatca gttcgtcagc tatggatttg
     6661 gcctggctca gcgcgtattt ttgcccgtcg ttctgcacct gataattcgc aaacgagcat
     6721 ttgctgtgca gaggctggat gcccgaacga ttcaggattt tttccacccg caactggtga
     6781 ttctggcggt taatctcctc gctgcgtttt cgtccttcaa caagttgcca ttcccgccac
     6841 tcctccaccg tccggtacgg tggaaccgac gcctgtggtg caagtctgcg aatacgttca
     6901 agaaccccgg ctgccgcaat gtttttcatg ccacatcacc ccctgaatcc cggcggaatt
     6961 tcggtatccg gttcagaaat atgattcaca caacgctggt tgttcgtgcc gcttaccggg
     7021 agcaaccagg ggttttcaaa attccggtcc ggcccaaaaa acgtcgtcgc tcgctgaaca
     7081 aattccgttc ccgttttccc ggtagccgcc aggtatcttg cgtaacgcct cacaccatcc
     7141 agtatggcct ctggtggcac cccctcgcgt aatctggcct tccaggcact gaaagcggat
     7201 ttcttcgggt ttgccccagc acgcaacggg tattcccgcc agacctgttc gaacacatcc
     7261 ggataatcca ctcgtcccac aggctgcccg gtgttttccg ggactacccg atcggcttcc
     7321 cgctgaatgg cggaatcggc ttcaggctgc tgcagttggt gtgattgctc cggccttgcg
     7381 gtcatcacct gctgcacagc gcccgaatcg gctttcagcg catacgctga atcggcttcc
     7441 ggtgtcgtgc ctgctggctg accaggattg acggtctgaa catcccctgc ctggttcgtg
     7501 gcgtttttta cgccatggac catagtgttt tgatcttctt gatctgtatc tttatctgta
     7561 tctttatctg tatctttatc tgtcgtgact cgtcgtgaca tgtgcgtgac atttcgtgac
     7621 gcgccgtgac aatcgccatt ttgttcccgc tttctttccc tctctcgctg cgccctcttg
     7681 cgctctgcag gagattttgc ggtttgcgaa atattgccgt tgtcctcttt aagcacctgg
     7741 cgtttttccc atccagtgat taaatcacca tcaagtaccc gcccctgcat cgtctgcaaa
     7801 attgaatcaa ttacctcttc tgtcacgtcg agcgcacttg ccaaatcttc tgtcgtgaca
     7861 tcaatgtgac ctcgcgtgac atttcgtgac gcgctcacca ggaggtggat atacactgcc
     7921 atcactgttg caattggctg ccctgacacc ctggcaattg ttcgccactt agggtcattt
     7981 ggcatgtcat gccataatct gagccaggcg ttagccatac tcacctcttc tgataccgaa
     8041 tctttttact cacgagttgc cggaagcgat tcgatacggc tattgtcagt caatgtactg
     8101 ccacagcatt tcctgccggg ccaccacggt tcatctgatt gaaaccggcg attgccactg
     8161 cgacaaaatc atcagcgtct ctcaccagtc gctcccgcgt ctccaccagc tcccgaaaat
     8221 aagctgaact gtggctgcgc attctggcca ccagcaaagg tggcattgcc ttttcgatcg
     8281 ctggtaacaa cgcctgaatt ttttcaactg catcaggggt gtctttctct acccagcgga
     8341 aaattttctg ggtattgcga gccagggctt ccggatggct gtcgtcatac agttcaggaa
     8401 acgtcatacc caactcaaaa taagcctggg ttattccagc tgccggaact ttttcgccat
     8461 caggacgcgc ccaggcattc atcgccatgc ggatgtgttc atgcttgatt ttcatgaatc
     8521 cccccttggt tagaaggcgg attatgatca gaaccgggaa tgacaaccgt cggtatgtgt
     8581 aactcatatt tgagcgcccc ggcagtgact gcctgaatta gcaacgccca tttccacgga
     8641 acctcttccc cccacatgct gactgtggtt tttgacgttc ctagagctgc ggctgtttta
     8701 acaactccgc caaaatagcc taatacttct gattttttca tgagtcgctc cataaaactg
     8761 aacgccaaaa gtttaataat caaaaccaaa gaaagtcaag aaacaaaacc atctgtgttt
     8821 taaaatcaaa acatgagcaa gcaaacaata tctgaacgca taacccaacg tatgcatgcg
     8881 ctaaacctga aaggcaaaga ccttgtcaat gccactggcg catcaaaagg ctccgtaagt
     8941 caatggatga acggtggagg agcgccgtcc tcgcgttaca taagttcact ggcaaaaata
     9001 ttgaaagtaa acgaaaattg gcttcttaat ggaggagagt taaatacagg tgattcgctt
     9061 gatctatctt taccgccgat aaaaacggtt ccgctactat cacttcagca ggcagcaagc
     9121 tggagtgatt atatgaaaaa ttcctcaata acctcttgtg tgcagcttgt cggagaaatc
     9181 ccggccaata cctttgcagt tgttctagag agtgacagta tgtcaacatc tggtggggga
     9241 gtttccatcc caaatggttc aacagttttt gttgatccca atcgaaccgt acaaccagga
     9301 aatattgtcc ttgccttacc caaagggacc acaacacctg tcattcgtaa actggagata
     9361 gaagggccgg atattctttt agtccccacg aatcctcgct acccttcaat tatgctggat
     9421 gatctatctt gcatattggg cgtatgcttt aaaattcaac aagatattta accgacctca
     9481 tctatttgat taactgtatg ccatcgtggt gatggcttaa cagctgcctg cttaaaatgt
     9541 tttgataaaa aaacattgac ctgaaaagtt catttttcta aacttcattc attccctctc
     9601 cccaccccac agaatgcagg gcaatacttc gagttaccag gcagtggtca ggggttaagt
     9661 agccagcccg aggcgtaaga acatgacggc agggttcaac tttaataact atgcagcagg
     9721 tttttgttcc gctaccccgg cgttaagggg aaatgaggtc agcatggata ctatcgatct
     9781 tggcaacagc gaatctctgg tatgtggcgt gtttcccaac caggacggta cgtttaccgc
     9841 gatgacgtat accaaaagta aaacgtttaa aaccgaagct ggcgcgcatc gctggttagc
     9901 aagaaactc
//
LOCUS       DAZFWP020000173         9887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFWP020000173 DAZFWP020000000
VERSION     DAZFWP020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.173"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            147..362
                     /gene="bdm"
                     /locus_tag="ACHK0Z_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             147..362
                     /gene="bdm"
                     /locus_tag="ACHK0Z_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU4504134.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            464..601
                     /gene="sra"
                     /locus_tag="ACHK0Z_RS21060"
                     /old_locus_tag="pgaptmp_004212"
     CDS             464..601
                     /gene="sra"
                     /locus_tag="ACHK0Z_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU4504135.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            758..2455
                     /gene="maeA"
                     /locus_tag="ACHK0Z_RS21065"
                     /old_locus_tag="pgaptmp_004213"
     CDS             758..2455
                     /gene="maeA"
                     /locus_tag="ACHK0Z_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4504136.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRMEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2589..3599
                     /gene="adhP"
                     /locus_tag="ACHK0Z_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             2589..3599
                     /gene="adhP"
                     /locus_tag="ACHK0Z_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU4504137.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3746..4030
                     /locus_tag="ACHK0Z_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             3746..4030
                     /locus_tag="ACHK0Z_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4504138.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4081..4734)
                     /gene="fdnI"
                     /locus_tag="ACHK0Z_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     CDS             complement(4081..4734)
                     /gene="fdnI"
                     /locus_tag="ACHK0Z_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU4504139.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIIEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIILRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4727..5611)
                     /gene="fdnH"
                     /locus_tag="ACHK0Z_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     CDS             complement(4727..5611)
                     /gene="fdnH"
                     /locus_tag="ACHK0Z_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU4504140.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5624..8671)
                     /gene="fdnG"
                     /locus_tag="ACHK0Z_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     CDS             complement(5624..8671)
                     /gene="fdnG"
                     /locus_tag="ACHK0Z_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8084..8086),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4504141.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLLWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKLLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8903..9784
                     /gene="yddG"
                     /locus_tag="ACHK0Z_RS21095"
                     /old_locus_tag="pgaptmp_004219"
     CDS             8903..9784
                     /gene="yddG"
                     /locus_tag="ACHK0Z_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU4504142.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2405 a         2592 c         2488 g         2402 t
ORIGIN      
        1 tatcaatatc atgaggttaa aatataaaaa tcatcaacca ggactaatct taacaacgaa
       61 gcggcaaata tttgccatgc tgaatgtgct ttataaatct gcgatccgta gcagacacca
      121 taaatacaca gacacggaga atcactatgt ttacttatta tcaggcagaa aattcaacag
      181 cagaacccgc tctggttaat gccattgagc aaggccttcg ggcagagcac ggtgttgtca
      241 ctgaagatga cattctcatg gagctgacca aatgggtgga agcttctgat aacgacatcc
      301 tcagtgatat ctaccagcaa acgatcaatt atgtggtcag tggccagcac cctacgcttt
      361 aaggtgctat gcttgatcgg caacctaatt taggggttta gcacgtgttt cttcgctacg
      421 gcgatgttgt ccttaaaact agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca
      481 ggcacgtcat attcttggac tggaccataa aatttctaac cagcgcaaaa tagttaccga
      541 aggtgacaaa tccagcgtgg taaataatcc aaccggcaga aaacgccccg ctgaaaagta
      601 attcataacc atcagtcctc aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt
      661 gtttttatct gctttatact tggggccgac gccctggcgg taaagcaaag acgataaaag
      721 cgtgccaggg atggatattc aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca
      781 gcgttcgctt tatatccctt acgctggccc tgtactgctg gaatttccgt tgttgaataa
      841 aggcagtgcc ttcagcatgg aagaacgccg taacttcaat ctgctggggt tactgccgga
      901 agtggtcgaa accatcgaag aacaagcgga acgagcatgg atccagtatc agggattcaa
      961 aaccgaaatc gacaaacaca tctacctgcg taacatccag gacaccaacg aaaccctctt
     1021 ctaccgtctg gtaaacaatc atcttgatga gatgatgcct gttatttata ccccaaccgt
     1081 cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt tcacgcggcg tgtttatctc
     1141 ttaccagaac cgccacaata tggacgatat tctgcaaaac gtaccaaacc ataatatcaa
     1201 agtgattgtg gtgactgacg gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg
     1261 gatgggcatt ccgatcggta aactgtcgct ctataccgcc tgtggcggca tcagcccggc
     1321 gtataccctt ccggtggtgc tggatgtcgg aacgaacaac caacagttgc ttaacgatcc
     1381 gctgtatatg ggctggcgta atccgcgtat cactgacgac gaatactatg aattcgttga
     1441 tgaatttatc caggctgtga aacaacgctg gccggacgtg ctgttgcagt ttgaagactt
     1501 cgcacaaaaa aatgcgatgc cgttacttaa ccgctatcgc aatgaaattt gttcctttaa
     1561 cgatgacatt cagggcactg cggcggtaac agtcggcacc ctgatcgcag caagccgcgc
     1621 ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg
     1681 cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc
     1741 gcggcagaaa gtctttatgg tcgatcgctt tggcttgctg accgacaaga tgccgaacct
     1801 gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac ctcagtgact gggataccga
     1861 cagcgatgtg ctgtcactgc tggatgtggt gcgcaatgta aaaccagata ttctgattgg
     1921 cgtctcagga cagaccgggc tgtttacgga agagatcatc cgtgagatgc ataaacactg
     1981 tccgcgtccg atcgtgatgc cgctgtccaa cccgacgtca cgcatggaag ccacaccgca
     2041 ggacattatc gcctggactg aaggtaacgc gctggtcgcc actggcagtc cgtttaatcc
     2101 agtggtatgg aaagataaaa tctaccctat cgcccagtgt aacaacgcct ttattttccc
     2161 aggcatcggg ctgggtgtta ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat
     2221 gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc ctgaacggcg aaggtctggt
     2281 actaccggaa ctgaaagata ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa
     2341 aatggcgcag cagcaaggcg tggcggtgaa aacctctgcc gaagccctgc aacaggccat
     2401 tgacgataat ttctggcaag ccgaataccg cgactaccgc cgtacctcca tctaagcctg
     2461 cgcccggtag tgaaggctac cgggctattt ccctctccct ttttcagatc tcatccatac
     2521 tgggtagtgg cgaataaatc tcatttgcct cacctgctat gcagaacatc atccgaaaag
     2581 gaggaactat gaaggctgca gttgttacga aggatcatca tgttgacgtt acggataaaa
     2641 cactgcgctc actgaaacat ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc
     2701 ataccgatct tcatgttaag aatggcgatt ttggtgacaa aaccggcgta attctgggcc
     2761 atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt cacctcatta aaaccaggcg
     2821 atcgtgccag cgtggcgtgg ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg
     2881 gtaacgaaac gctctgccgt tcagttaaaa atgccggata cagcgttgat ggcgggatgg
     2941 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggc ctggactcgg
     3001 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aaactgtcaa
     3061 aaattcgtcc cggtcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     3121 tgcaatacgc gaagaatgtc tttaacgcga aagtgatcgc cattgatgtc aatgatgagc
     3181 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3241 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc tgcggtggta acagcagttg
     3301 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3361 tcggtctgcc gccagagtct atgagcctgg atatcccacg ccttgtgctg gatggcattg
     3421 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3481 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3541 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgccgttaag
     3601 aggcctttgc tgcgagtgcc agtcctggtg tcgcagcatc gcactctctc cgacattaaa
     3661 tggctaaatg gattattgtt ccttcctacg caacaataat gatagtgttc ctgatgaagg
     3721 aacactactg agagtaaaga cactgatgaa aatggccaat catccccgcc cgggggacat
     3781 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3841 aattgctccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga caccagaaat
     3901 ggcgattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3961 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgactac gccgtctggt
     4021 tacgcaataa aaaaatggcg cttcttaaag cgccattttc agcttaaagt tcaaaagaga
     4081 ttatatccct tcttcactct cttttttcgc ttctgccttc tcgatttcac gataccagcg
     4141 cggatggtgt ttcttcgccc agcgacggct caccttccct tcgatcatcc ctttaatcga
     4201 ccctttcacc cagaatgcca tatacatatg gatcaggatg gcgtggatca ggatgatacc
     4261 cgcagccgcg tggatcagca ggctgtagcg aacaacctgc atcgggaagt actgcgcaaa
     4321 gtacggacgc aagataatca ccccggtcac cagcagcacg aaaatcatgc tcatgatcga
     4381 ccagaacatc atcttttgcc cggcgttgta cttaccgaca tccgccactt tatgctcatt
     4441 gcctttcaat acttcgataa tgttcaacag ccacggaata tctttcttat ccgggatgtt
     4501 gtgatgcaca aaacgcacaa acataaacat cagtgcgacg aaaatcgcaa tgccgaaaaa
     4561 cgggtgcaaa atgcgtccca tctgcggcgt accgaaggtt tgcgtcagcc attgcagcgt
     4621 cgggaagaag aacgaaatcc cggacagcgc caccaggaag aagcaaatca ccacggtcca
     4681 gtgacaggcg cgatcaataa atttggtgcg cacaatcatt ttcgacttac tcatgatgat
     4741 cctcctcgtc atcgtccact tccttattcg ggccaatacc gatgtagtgg aaaatcaacc
     4801 cggcaaaagt ggcaataaag ccagccgctg ccagcggttt caacgcgcct ttccacaggc
     4861 ttaccgaggt gtcgatcttc ggatctttcg gcagaccgtg atacagctcc ggctgatcgg
     4921 cgtgatgcag cacgtacata acgtgcgtac caccgacccc ttccgggttg tagacgccag
     4981 catgttcgta accacgcgct ttcagtttcg ccacgcgctg ttccgccagc tccagcatct
     5041 ccttcttggt gccgaagtgg atagccccgg tcggacaggt tttcacacaa gccggttcct
     5101 ggccgacgct gacgcgatcg acgcagagcg tgcatttata tacccggtta tcctctttgt
     5161 tgaggcgcgg aatattaaac ggacacccgg caatgcagta accacagccg atgcagtttt
     5221 ccgactggaa atcgacaatc ccgttagcgt actgaatgat tgcaccagca gacgggcacg
     5281 ccttcaggca gccgggatct tcacagtgca tacagccgtc tttacggatc agccactcca
     5341 gcttgccgtt ctgttcggtt tcgctaaagc gcatcaccgt ccaggacttg gcgctcagat
     5401 cggcggggtt atcgtaaacc ccgacgcagt gccccacttc atcacggatg tcgttccact
     5461 ccgaacacgc cacctgacag gctttacagc cgatacaggt ggaaacgtcg ataagttttg
     5521 cgacttctgc tttgtaatca cgcacctgag aaggcggcgt gatggagtta gttgcggacc
     5581 ttttgataat gtcctgcgtt tccatagcca tctgttcgcc cccttacgcc ttctcgatgt
     5641 tgactaagaa cgctttatat tccggcgttt gcgagtttgc atcaccgaca ttcggcgtca
     5701 gagtgttagc gatataacct ttacgcgcga caccctcaaa gccccagtgg attggaatac
     5761 ccaccgtttc aacctgctga ccatttacat tcagtagttt cagacgacgc gttaccacag
     5821 ccaccgcgcg gataaagcca cgcttgcttg agacagtgac acgatcgcca ttattaatgc
     5881 ctttcgccgc cgccagcgtt tcgctgattt ccacaaactg ttccggctga gcaattgcgt
     5941 tgagcaatgc gtgcttggtc caggtgtgga agtgctcggt cagacgatag gtcgtaccca
     6001 catacgggaa ctgctctttt ttacccatcc gcagtgcgtc ttgttcatac agacgaacaa
     6061 ccgggttaga caccacgttc ggatgcagcg ggttagtgcc cagcggcgtt tcaatcggct
     6121 cgtagtgttc cgggaacgga ccttccgcca ttttgttgat ggcaaacagg cgtcccatcc
     6181 cttccggctg catgataaac ggcccggttg gcgtacccgg tgcggcattg ccgaagtcag
     6241 gaatatcgtt acccgtccac ttgctgccgt tccactggat cagcatccgt ttcggatccc
     6301 acggtttacc gttgatatcc gccgaagcac ggttgtacag cacgcgacgg ttgagcggcc
     6361 acgcccaggc ccatcccagc gtattcccca gaccggacgg gtcggagtta tcgcggttag
     6421 ccatctggtt gccctgctct gtccagctac cggtgtagat ccagcaagaa gatgcggttg
     6481 taccgtcatc acgcagatgc gcaaagctac tcagcaactg acctttcttc gcaatcagca
     6541 cgccattagc atcatagaga tcttccagcg catagccgtt gttctcttta gctacttcgt
     6601 cagattgcgg ttcgtgcggc tgcttgtagt tccagctcat cttcatcagc ggttctacgc
     6661 ctttaccacc ttcggcctgg tacagctcgc gcaggtgatg gtagataccc gccagaattt
     6721 caccgtcgtt acgcgcttcg cccggcgcgt cctgaccttt ccagtgccac agcagccagc
     6781 gaccggagtt agcaatagaa ccatcttctt cagcaaagca ggtcgaaggc agacggaata
     6841 cttcagtctg aatagacgcc ggatcgacat cgttcgactc gccgtggttc tgccagaagg
     6901 ttgaggtttc agtcaccagc ggatcgataa ccaccatgta cttcagcttg cttaggcagc
     6961 tcaccacttt gtttttgtcc gggaaggacg caaccgggtt aaagccctgg cagaaataac
     7021 cggtgacttt gccttcatcc atcatgttga aatacttgat gacgtcgtag gtctggtccc
     7081 acttcggcag ccagtcatag ccccagttgt tctctttctg cgcggcatcg ccatagaaag
     7141 atttcatcag gctaacgaag aacttcggat agttgctcca gtagttcacc tgaccagcca
     7201 gcgtcgcttt cggcgtgttc gcttccagat acgactgcaa atcaacctgt ttttctgacg
     7261 gcagcgtcag ataacctggc aggctggtag agagcagacc taagtcagtc agaccctgaa
     7321 tgttggagtg accacgcaat gcattcacgc cgccaccggc catacccatg ttaccgagca
     7381 gcaactggat catcgccata gtacggatgt tctgcgcacc cacggtgtgc tgcgtccagc
     7441 ccagcgcgta caggaaggtg gttgtgcgat ccggtgcgct ggtggaggcc agcacttcac
     7501 acactttcag gaagtcggct tttggcgtac cgcagatgtt ttcaacgacg tctggcgtgt
     7561 agcgggaaac gtgctctttc agcaggttcc acacacagcg cggatgagtc agtgtttcat
     7621 cgcgtttcgc atagccgttt tcatcgaact gatagttcca ggacgatttg tcgtactggc
     7681 gtttttcagc gtcataaccg ctgaacaggc catcttcgaa agcaaaatca tcacgcacca
     7741 gcaggctggc gttggtgtaa tgtttaacgt attcggcgtt gattttgttg ttttcgatca
     7801 ggtagcgcaa aacgccagac aggaacgtaa tgtccgtacc ggaacgaata ggtgcgtaaa
     7861 tatccgccac agaagcggta cgcgtaaaac gaggatcgac aacgatcaag gttgcatcgt
     7921 tgttgttttt cgcttccatc gcccagcgga aaccgacggg atgcgcttca gcagcgttac
     7981 cgcccattac cattacgacg ttagcgtttt tgatatccac ccagtggttg gtcatcgcac
     8041 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagacg cgcgcctggt
     8101 tgtctaccgc cagcatcccg agggagcggg caaatttctg tgtcagcatc ccggtttcgt
     8161 tgctggcacc ggaggcacac agcataccgg tagaaagcca acggtttacc gttacgccct
     8221 gctcgttctt ttcaataaag ttagcgtcac ggtcagcttt catcagcttt gcaatacggg
     8281 agaatgcttc ttcccagcta atgcgctgcc atttgtcaga acctggcgca cgatattccg
     8341 ggtagcgcag acggttttcg ctgttgacgt aatccagcaa accggcccct tttgggcaca
     8401 gcgcaccacg gcttaccgga tgatccgggt caccttcaat gtgataaatc gcttctctgg
     8461 cgtttttcgc gccatcaccc aggctataca tcaatagccc gcaacctacg gaacagtatg
     8521 tgcaggtgtt acggatctct ttagcgcgta ataatttgta gtttcgcgcc tgagccagtg
     8581 cttgcttcgg ggcaaagccc aatgccgcta ccgttgttcc agccataccg cccgcgcaga
     8641 ttttaaaaaa ttgtctgcga ctgacgtcca ttgctttcct cttttttcag gggtattact
     8701 gcgcgaggaa actaacagta aaattctgaa tctttttgcg cgaaatcaaa aagaacacgt
     8761 gtttgccaca aaataatccg cctgcgcaaa ttgacctacc tcaatagcgg tagaaaaacg
     8821 caccactgcc tgacaggcca gttaaaaaaa tgctataaaa ttcagcttaa tttttaacgg
     8881 caagagagac aaaacagcga gcatgacacg acaaaaagca acgctcatag ggctaatagc
     8941 gatcgtcctg tggagcacga tggtaggatt gattcgcggt gtcagcgagg ggctcggccc
     9001 ggtcggcggc gcagctgcta tctattcatt aagcgggctg ctgttaatct tcacggttgg
     9061 atttccgcgt attcggcaga ttccgaaagg ctatttactc gccgggagtc tgttattcgt
     9121 cagctatgaa atctgtctgg cgctttcctt agggtatgcg gcgacccgtc atcaggcgat
     9181 tgaagtgggt atggtgaact atctatggcc cagcctgaca attctctttg ccattctgtt
     9241 taatggtcag aaaaccaact ggctgattgt accaggatta ttattagccc tggtcggcgt
     9301 ctgctgggtg ttaggcggtg ataatgggtt acattatgat gaaatcatca ataatattac
     9361 caccagccca ttgagttatt tcctggcgtt tattggtgcg tttatctggg cagcctattg
     9421 cacagtaacg aataaatacg cacgcggatt taacggaatt accgtttttg tcctgctaac
     9481 gggagcaagt ctgtgggttt actattttct cacgccacaa ccagaaatgg tatttagcac
     9541 gcccgtcatg attaaactca tctctgcggc atttacctta ggatttgctt atgctgcatg
     9601 gaatgtcggt atactgcatg gcaatgtcac cataatggcg gtaggctcgt attttacgcc
     9661 cgtactttcc tcagcgcttg cagccgtgct gctcagcgcc ccgctgtcgt tctcattctg
     9721 gcaaggcgcg ctaatggtct gcggcggttc cctgctctgc tggctggcga cacgtcgtgg
     9781 ttaaaataat tgccgggtct acggcccggc agttaatcag ataaattccg acaatattaa
     9841 aaaataaatt tcaaatacca gtaattcacc ttatatctca tttcgac
//
LOCUS       DAZFWP020000174         9848 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFWP020000174 DAZFWP020000000
VERSION     DAZFWP020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.174"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..164)
                     /locus_tag="ACHK0Z_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             complement(<1..164)
                     /locus_tag="ACHK0Z_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGU4504143.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWR"
     gene            complement(321..809)
                     /locus_tag="ACHK0Z_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             complement(321..809)
                     /locus_tag="ACHK0Z_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4504144.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            complement(822..3629)
                     /locus_tag="ACHK0Z_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             complement(822..3629)
                     /locus_tag="ACHK0Z_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4504145.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETPFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLASNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEVDRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATIRSLSLVIGVLYGKLATLRLGFDILTRS
                     MNVVRVLPALWGMLTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILRD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(3616..3771)
                     /locus_tag="ACHK0Z_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             complement(3616..3771)
                     /locus_tag="ACHK0Z_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU4504146.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(3780..4154)
                     /locus_tag="ACHK0Z_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             complement(3780..4154)
                     /locus_tag="ACHK0Z_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4504147.1"
                     /translation="MKDIDTETRNNTVADDVTAGEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFMLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4210..4722)
                     /locus_tag="ACHK0Z_RS21125"
                     /old_locus_tag="pgaptmp_004225"
     CDS             complement(4210..4722)
                     /locus_tag="ACHK0Z_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGU4504148.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(4722..5906)
                     /locus_tag="ACHK0Z_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             complement(4722..5906)
                     /locus_tag="ACHK0Z_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4504149.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            complement(6329..6412)
                     /locus_tag="ACHK0Z_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /pseudo
     CDS             complement(6329..6412)
                     /locus_tag="ACHK0Z_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6373..7173
                     /locus_tag="ACHK0Z_RS21140"
                     /old_locus_tag="pgaptmp_004228"
     CDS             6373..7173
                     /locus_tag="ACHK0Z_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HGU4504150.1"
                     /translation="MTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQI
                     SEMLMDIEIDHADQTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLP
                     SFAITRSSGDRHQFRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSR
                     EGDYMEGAEGNVFVLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEM
                     HGTVTGFKSEIDNQDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            7216..7476
                     /locus_tag="ACHK0Z_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     CDS             7216..7476
                     /locus_tag="ACHK0Z_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4504151.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            complement(7615..8703)
                     /locus_tag="ACHK0Z_RS21150"
                     /old_locus_tag="pgaptmp_004230"
     CDS             complement(7615..8703)
                     /locus_tag="ACHK0Z_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HGU4504152.1"
                     /translation="MQMQHLMVGYPKYYQTADYALRLSVMADIATMRMKALHFWDKHG
                     ISAASEAFGVSCRTLYWWRQLLNKEGPEGLIPHSKAPLVRRKKHWHPDVLKEIRRLRT
                     ELPNLGKEQIFVRLKPWCEQRYLACPSVSTIGRMIAAAHDKMRMIPVRLGSRGKALLV
                     KKRSAKPRRPKHYRPVKTGELIGMDAIELRMGELRRYVITMIDECSNYALALAVPSLN
                     SDIVNHFFSRAARLFPVGISQIITDNGKEFLGNFDKTLQEAAIKHLWTYPYTPKMNAI
                     CERFNRTLREQFIEFNEILLFEDLALFNQKLAEYLVLYNSKRPHKALALMTPVEYILK
                     ENKNCNMWWTHTSACSFVGEALTYHLLG"
     gene            8767..8907
                     /locus_tag="ACHK0Z_RS21155"
                     /old_locus_tag="pgaptmp_004231"
     CDS             8767..8907
                     /locus_tag="ACHK0Z_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4504153.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(9097..9378)
                     /locus_tag="ACHK0Z_RS21160"
                     /old_locus_tag="pgaptmp_004232"
     CDS             complement(9097..9378)
                     /locus_tag="ACHK0Z_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504154.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRTGGYDVVCYESDVTDEMIRQWKQENAE"
BASE COUNT         2417 a         2748 c         2371 g         2312 t
ORIGIN      
        1 aaccgccacc agaaaaggaa attccggtta cgctgctttt tctctcttaa aagcctccag
       61 atatccatac gtttccgccg aattacttca tgtttccaga agttttttaa attagtagag
      121 ttcccaaata aaacaaagtg aattgccatt aagtaagaca gcacgaaaat ctccttaatt
      181 attatttcag accacacatg ttataaggtt aagagattat aaaatcctgt tatttgttat
      241 tcaaaaacaa ttttctgaga aggacataca acagcaagtc gccagtcacc ttcatcagga
      301 aattggcgac atacgttaaa tcagagcagc cccttaactg agctggccgc gctattaagg
      361 gatgatgtca ccttatcttt gaagccggac aacatatcgc tgaacgatga ggattgcagg
      421 cgctcccgca aatcctcatc acagcgttca agagtcagtg aaaattctat ctttttcgcc
      481 ttaccgtagc gatcaaactc ggagcgggtc gtattcgttt cagtcaggac atacatgccg
      541 taaatctgcc cgacgccatc aatcaaaggc cagggtcgtc ctgtatacgc ctgcgtggtc
      601 agcagcgaca gcgacacttc gccacctgta atttcaggat aaagcacacc agaaagaacg
      661 atgcgatcat cacctgcacc gatatactgc cagcttgctg aacggttaac gcgttcattt
      721 ttcacatgcc gccagctttt gttttgctgt aactgctgat gcggcagcgt gcgcagctca
      781 aaaacaaaca tgccgtagat catcatcatg gccatgactc ctcaatcttt atcgtaaaaa
      841 ctgccacgcc cggcacgggc acgccgttcc atttctgccc tgaccatttc accgaccagt
      901 ttcgccagtt cgcggggatt ctgcgtaaca acgttatgca gatgaacatg aatttcacca
      961 ccaaatccgg aggcaacagg ctcccggtta cgggaagtta caggaactga tgccactgga
     1021 gatcgtatgg cctccgccac cgggcgggag ctggccgcaa caacagggac cagcgccgga
     1081 ggcagcggtg ccgggaccac gggggtgata ttaattgcgg gggcaggctt actgacctgc
     1141 gcaatcttcc gctcctgcca ctccccacga acagcaagtg cgcggggcag gttcttaaag
     1201 acaatatcgc cggggccaat gcgttttttc gtctcatcaa ccagcttacc tgtgttatca
     1261 gcaattttgc tgagtctgcg tagcgtcccg gtattgctgt ctgtgagcgg tttgttgtct
     1321 ttgggtttat cacctccggt gccattgcca ttttccacag gcttcggcgg attgattttc
     1381 gccaggtccc cctgaagtaa ggcaaccttg tcccgaagaa tggccgcacg ctgtgcgtct
     1441 tcgattttct tgcgcgccct ttcagcttca tccggaagga cgccaagttt ttcaagtatc
     1501 cacgccagcg tatccagtag catttttgca ggtgtcagaa caagttgtaa cgcaccgcca
     1561 agaacgttac cgaatatctc gccagcactg gtacatttat ccagcgtttc cttgctggac
     1621 tccatcggtg acagcagcga tttaaaccag ttaaacacct ggctgatccc gcttccgatt
     1681 gcgtcaaaaa cagggccaaa ccgttcaaag gtttcgcgca acggggtcag cctttccata
     1741 atcccgctga acaccccggc aaaaaatgcc ctgatgggat cccagtattt ccagataaga
     1801 acggcagctc cggcaagcgc agccacgata agaccaaccg gactgaacag cgccccgata
     1861 gcgcctccca gcaaagaaac ggaacccgtc agcattcccc acagcgcagg caacaccctg
     1921 acgacattca ttgaccgggt aagaatgtca aaaccaagac gcagggtggc cagctttccg
     1981 taaagcaccc caataaccag cgacaacgag cgaatcgttg cagtcattgc cagcaacgca
     2041 ccgcctgcta tcagtagctg gcgtgtcagt accggatggg cctgcgccag cgaggtgatt
     2101 ttttcaagca cccgcgtgag ccactgcgtg acagaacgca gcggaccgtc aaccagatca
     2161 ctgatgcgaa tacgaagacc ttcccatgcg ctgtcgagat ttttcaggtc cccatcaaga
     2221 ttatcggcca ttacttttgc gacgcgatcg acctctcccc ttgccccctg caattctctg
     2281 gtcagttttt gcagctctcc tgaaccagcc gccgcaacaa gcgtctgcaa accaacgaac
     2341 gcctcttctc cggcgatgtc cttgaagaag gaaacctggt ccacctgtcc gtatttttgt
     2401 gtcgccttat agagatcaag cagcacatcc tccatcgggc gcattttgcc cctggcgtca
     2461 gcaactgaca cccccagctc tttcagcgca tcagccgcag cttttggcgg tgatgcaagg
     2521 cgggacagac ttgcgcgcat ggccgtacca gcatcgcttc cacgaagacc attactggca
     2581 agcatcccgg ccatggctgc cgcttcttca agactgatac caagttttgc ggcaaccgga
     2641 ccggtatact tcatggtttc gcccagcgcg cgtaaatcag tattggtccg ggtgaatgcc
     2701 gctgtcagcg tatcgccaac ccggtccatt tgatcggctg tcaggttgaa ctgtgtgagg
     2761 atattggagc ctatatcagc cgtctcgccg agttcgacgc cacctgccag cgccatatta
     2821 agaacaccgg gcaatgcggc ctgaatggcc tgcggagtaa aaccagccat tgccagaaag
     2881 ctctgcccac tggcggcatc actcgcagta aacggtgttt cagatccaag ttttaacgcc
     2941 tgctcacgca gcgccttaaa ctgcgggctg tttttgtcga ttcgcgtcag tgcctgaacg
     3001 cgggacatct ctttgccgaa cccgatcgca ggctgcaaaa aacgcccggc agcatagccg
     3061 cccgccgctg ccgcaccaat tgccagtgca ccacctgttt tcagttttcc cgctgtttcc
     3121 tgcgcgcgcg aataccgctc acgcgcccgc gttacacgcg caagcgcctg ccgttcgcgt
     3181 tcaagctggt tgttgtactg ttcggtgcgt ctgatggcct gctggatggt gttatcgctg
     3241 cctgtcaggg aaatgccgtg gcgtttcagc tctccgccaa gctcccgcat tttttgaatt
     3301 tcccgtgtgc gcgattcatt caggcgttca agccgggtgc ttaactgctg catcagcttt
     3361 tgttgttttt cgctgagcac tgtacccgta cgttgtaact gattaagggc gttaagctgg
     3421 cgtcgtgctt tcacgatgcc agcatccgct ttactgacag cgtcacgggc gcgctcaaat
     3481 gaacgcgcct gacgctcgag atttttaatc gccccctgcg ttcgctggat ggagtcacca
     3541 aactgcccca tcaggcggcg ggcgttttcg gcaggccggg tcagcctgtc aacggcgctg
     3601 aaagcgaccc ggatatcaag agtcttcatt atctgcattc ccgctgcgaa gtgccgcccg
     3661 ctcgcgccag ctaaccactt cgccgggcgt catcatgaag atttcggcgg gcgaccagtt
     3721 aaaaatggcg gcaatatccg ccaccagatc ttcgatgtgc tcaaagcaca ccagggtgat
     3781 tacgctgccg tctcctgcac gctcttcgcg ccagagtctg gctcgctcat aaaatttaca
     3841 gccacagcgc acaactgaat aaaatcgcgt gacgacattt ttttaatcat cacttcatcc
     3901 agtcgtggcg aggtcacgcg aggcaacagc ataaacatgg tatccgcttt cagattcagc
     3961 acatcagaca gcgacagacc acgcagggat ccagcctgct caatagcccc ggtgatctcc
     4021 acatacgtga ttttttcgcc accacgctca attggtcggg tcagttttac gccacgttcg
     4081 acagccatat cctcacctgc cgtcacatca tccgccacgg tgttattccg ggtttcagta
     4141 tcgatgtctt tcatcagttg tctccttttc agtcagaggc gacgcactgc gccgcctgca
     4201 tattacttat cagccaagcc caagcgcgga acggatacgg tcaggcacaa tgtccttgcc
     4261 gtccttccgg tagatgtggt tcaacaggtc gatttcccac agcgggcgat cgttaacgct
     4321 cagcttgtag taggtgtttt tgacagcgta agtgtgtgat gtggcttccc cctgtttggc
     4381 ttcccccata tcaatttccg tcacacgccc gcgcatctcg atctcataca gatcgctttc
     4441 tgcatcggtg tagtattcac ccgcaaaacg cagcagcgtg ccgtcaatcg tgccgccata
     4501 tttaaggaac agcgcacgaa cagctccccc cataacaaaa ctcgcatcaa gcgcggagtc
     4561 gtccagaccg agatcaatac ttaccgcccc catcatgcca ccaccacgat agctgtcggt
     4621 tttgcgcgtc agtttgggcg gcgtgacgga tgtcactttg cccacttcgt tttcaccatc
     4681 cacaaacaac gtaaaaaagc gaagatgttt tggcacagcc atcaggcacc tcccagcacc
     4741 gcaaatgcgg gaccaaagaa ttcatcggta aacgtctggt aaagctccat gtcttccagt
     4801 ggcggaacgg gcgtatattt gtagcgaata cgcacacgtc cctgacgtaa atccgtggtg
     4861 ccgttatcca ccacgtcata ccagcactcc gcaccaatca gtttcccggc agtaaccagc
     4921 gaatccagtt ttgccctgat ggcactgata acatccttca cgttcgcagg cgtcagtgga
     4981 ctgtcgatgg tttcaaactg cgcttccgca attgaatcag ccagcacctg tgcggttcgg
     5041 gtatacacct caaagatgta ggcgttcgtt tccggtgtgc ggttgcccca gaagcggaac
     5101 ccgttgcgac gaataatggt cgtgatttct ttgttattga ggctgttggc atcactgtct
     5161 tcggcctgca acgaccagaa cacatgcctg gacattccca gcacattttt aaccggaaca
     5221 ttggacagcg atttgtgcca gccctgctca tggtcaatgt acgcacgaag gccgcacgca
     5281 taagcaggcg cggggaacgt ttcgtttttg ccacttttcg ggttgtaggc gatgaagtca
     5341 ggccataaga gcatcacctc acgttcgttg aatttctggc ggtaggtaat cgcctcagcc
     5401 atcgtgttac aaccatgaca tgtggcatac acaaacgcgc gcagtttacc cgcaatcacg
     5461 cacagggatt ttgtcaccgc ctccgtgtcc agctccggcg cggccagaat acgcggacgg
     5521 tatccgatgc tttcatcctg ctctgcaaca agcagcgcat acatccccgt atagctgccg
     5581 tcatcctcag aaccaccgat aaccagttga tcctgcgtct taccgtcttc ttctttgtgt
     5641 tcagccacgc gaacgacgat cacctttgtg ctcacctggt ctgcgatggc cttaagcgca
     5701 cgataaagcg tccccgttgt tccgcatttt cccagcacgt cattgacgcg ggtcagcagt
     5761 gtaggcttgt tcagcgggaa cagcttcgcg tccgcatcat ccgccgttgc cacgataccg
     5821 ataacgctgg aatcaacatc gttaatcgct gttaccaggt cggtattttc cgtaacacgg
     5881 gcaccatgaa aacgagtttc actcatagct tcagcccctt gtatccgtta aatgattcgg
     5941 caacaatcat cacccaccac gcgcgtaatc tcacccctgc gccgttctcc cgacccggcg
     6001 acaacaaaaa gcagtaaccc cctccgcacg cacatgcgac catgccgcac agggagggaa
     6061 cagatgaccg acaccaccat gcaattgctc agtcagggca cagaccccgt aaaaatgccg
     6121 gattttgata ttctcgcgga gggtaaaacg ctgtccggcg tggcagagcg cctgatgagc
     6181 ctgtcgctga ccgacaaccg gggatttgag gcagaccagc tcaccatcac gctggatgat
     6241 gcggatggtc agttgcggct accgccacgg ggcgcgcgcc tgacggttct cattggctgg
     6301 aaaggcgaac cgctgacaga aaaaggctct tacattgttg atgaaatcgc acacgaagga
     6361 ccgccggaca ggctgactgt ttcagccaga agcgcagatt ttcgggatga atttaacgtt
     6421 aaacgtgagg tgtcctggca tgatgtgacc gttgagcgtg tggtatccgc catcgctcat
     6481 cggtacggtc tgaaaccgca aatcagcgaa atgctgatgg atatcgaaat cgaccacgcc
     6541 gaccagaccg aagaaagtga catgtccttc cttacgcgca tggcggaaat gctgggcgca
     6601 atcaccacgg taaaaagcgg taatctgtta ttcatcatgc ccggcggtgg cgtgaacgca
     6661 cagggccagc cgttgccatc gttcgccatc acacgcagca gcggcgatcg ccatcagttc
     6721 cgcattgctg accgcgaggc gtatacgggg gtacgcgctt actggcttga tcttaattac
     6781 gggaaaaaga aaaaagtcag cgtgaaacgc cgcaaaccgc caaaaccgaa aaaagagaaa
     6841 agcagcagcc gtgaaggtga ttatatggaa ggcgcggaag gcaatgtgtt tgtgttacgc
     6901 aagacttatc agaacgagca ggcagcaaga cgcgcagcgg cggcaaagtg gcaacaacta
     6961 caacgcggag ccgcatcatt ctccatcacg ctggcacgtg gacgagcaga actctacccc
     7021 gaaatgcatg gcacggtaac aggatttaaa agcgagattg ataatcagga ctggattatt
     7081 gcaaaagccg agcacaccat tgataacagc ggctttacca cgcagcttga gcttgaggca
     7141 aaaatcccgg aatggatagc ggaaacagag tgagcaactt agaatagcgg cagcaccacg
     7201 ttaaaggagg tcgctatgtt ccgttgtccg ctttgtggcg catctgcccg tatccgcacc
     7261 agtcgtccgg aaaatgattc aaacaccgtg cggcaaaagt attaccagtg taacaacctg
     7321 gaatgcggcg tatgcttctc aacactggaa gctttccata aattcacatc gaaacacgcc
     7381 tccggcgttc actcatcaga aggtatcccg tggcatgagc tgccagcttc acacagggga
     7441 aacaatcaga tgagtttgcc tttacctcag aattaacagg cagaattgcc ggagtaacaa
     7501 aaaagcgata gattacgtgc gggtgccttt cggctgatgg tcggagggaa tacccgaagg
     7561 ccggatgtgg aaaggccccg gaaaacattt ctgtttaacc gaggccctaa cccgtcaacc
     7621 tagcaagtga taggttagcg cctctccaac aaaggagcaa gcgcttgtat gggtccacca
     7681 catattgcaa tttttattct cttttaaaat atattccacg ggcgtcatta gtgcgagcgc
     7741 cttgtggggt cttttgctgt tatacagcac cagatattcc gccagcttct ggttaaatag
     7801 cgccagatct tcaaagagta aaatctcatt aaattcaata aactgctctc ttaacgtccg
     7861 gttaaaacgt tcacagatag cgttcatttt tggcgtgtag ggataggtcc agaggtgttt
     7921 gatagcggct tcctgcagcg ttttgtcgaa gtttcccagg aactcttttc cgttatctgt
     7981 gattatctgg ctgataccga ccgggaacaa ccgggctgcg cggctgaaga agtgattgac
     8041 gatatcactg ttgagtgacg gcacagccag cgccagcgcg taattgctgc attcgtcgat
     8101 catggtgatg acatagcgac gcagttcgcc cattctgagc tcaatggcgt ccatcccaat
     8161 gagctcacct gtctttaccg ggcggtagtg ctttggcctg cggggtttgg cagaccgctt
     8221 tttgacaagc agtgccttcc cccgcgatcc cagacgcaca ggtatcatcc gcattttatc
     8281 atgtgcggca gcgatcattc tgccgatggt ggaaacactc ggacaggcca gatagcgctg
     8341 ttcgcaccag ggcttcaggc gaacaaaaat ctgctcttta ccgaggttcg gcagctctgt
     8401 cctgaggcgt cgaatctctt tcagcacatc gggatgccag tgtttttttc gtcgcaccag
     8461 aggtgcctta ctgtgcggga ttagcccctc aggtccttcc ttgttcagta actgacgcca
     8521 ccagtaaagc gtgcggcagg acacgccaaa ggcttcagaa gctgcagaaa taccgtgttt
     8581 atcccagaag tgcagagcct tcattctcat tgttgctata tcagccatca ctgaaagcct
     8641 caacgcataa tcggccgttt ggtagtattt aggatagcca accatcagat gctgcatttg
     8701 catagtgcaa tctcccctta cttatccgga ggcaattgca atatctctgt gaacttacac
     8761 agcgctatgt cgcaaaaatc gcttacggcc atcacgttct gcgtgacggt aatcctcatc
     8821 atctggatgc tgcacggttc gctgtgtgaa atacggatga gcttctgggg agcggagttt
     8881 gcggcgttct tacagtgtaa gcagtaagga aaccgcgacg gggaggcaac tccccgtcaa
     8941 tcggttgcta gggtaaggcc gataaggcac cctatctcat aggcatgaat aacaaccccg
     9001 cagcgtaaaa actgcggggt ttctttttgg aactctcact agctagtgag ccgttaactg
     9061 cacataatgc tcgcacaaca aatcagttaa aagagattac tcagcattct cctgcttcca
     9121 ttgccggatc atttcatcgg taacatcact ttcataacat accacgtcat accctccagt
     9181 acggctatat gcactgcgtc caccacatct actaccattc cttgcatgat tatacggaca
     9241 cgcacaattg cctggataag attcaatgga ttctttaatt atttctttct tgatctgagc
     9301 atctgacttt cctgttgctg catacccact aaaagatacc aaacaaaggc agattgccat
     9361 taataaacca catcgcatac cgatacactc tattactaac caatcatagc ccacatcata
     9421 aaatcgagat gaggagtaca acacctttaa caagaaggtg ccatcacgat aatatgccgt
     9481 gcaattcatc aaaaaaaaga tctatatcaa agagatagag ttattatcag tgctgcagac
     9541 atcagcattc gaggcagcag aaagacagta aatctaacga atttgtcttt ttctcaggat
     9601 gttgtcagat taaaaaagat agagtagcgc agacaccttt tggtggatgg taggaggaaa
     9661 aatgaagttg agtgggaaga tcccagaaaa aactttggtt taaccaaggc cctagttcaa
     9721 tatgcgaaat gcagatgagt tgcaggagag ttttgcttca cccttagagt ttaaacagac
     9781 gtgtactatc attatttctg catatcgcca caccatcgcc actttaccgc catttaacaa
     9841 aatacaaa
//
LOCUS       DAZFWP020000175         9747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFWP020000175 DAZFWP020000000
VERSION     DAZFWP020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.175"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(45..479)
                     /locus_tag="ACHK0Z_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             complement(45..479)
                     /locus_tag="ACHK0Z_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4504155.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(472..666)
                     /locus_tag="ACHK0Z_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             complement(472..666)
                     /locus_tag="ACHK0Z_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4504156.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            complement(663..1268)
                     /locus_tag="ACHK0Z_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             complement(663..1268)
                     /locus_tag="ACHK0Z_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4504157.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(1268..1990)
                     /locus_tag="ACHK0Z_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             complement(1268..1990)
                     /locus_tag="ACHK0Z_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4504158.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTREAA"
     gene            complement(2065..2799)
                     /locus_tag="ACHK0Z_RS21185"
                     /old_locus_tag="pgaptmp_004237"
     CDS             complement(2065..2799)
                     /locus_tag="ACHK0Z_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU4504159.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVLEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIY
                     EPDGPTNLLAWERLKNKELPPSLHRY"
     gene            complement(3074..3256)
                     /locus_tag="ACHK0Z_RS21190"
                     /old_locus_tag="pgaptmp_004238"
     CDS             complement(3074..3256)
                     /locus_tag="ACHK0Z_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4504160.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(3253..3780)
                     /locus_tag="ACHK0Z_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             complement(3253..3780)
                     /locus_tag="ACHK0Z_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4504161.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(3777..4223)
                     /locus_tag="ACHK0Z_RS21200"
                     /old_locus_tag="pgaptmp_004240"
     CDS             complement(3777..4223)
                     /locus_tag="ACHK0Z_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4504162.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(4180..4416)
                     /locus_tag="ACHK0Z_RS21205"
                     /old_locus_tag="pgaptmp_004241"
     CDS             complement(4180..4416)
                     /locus_tag="ACHK0Z_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HGU4504163.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            complement(4427..4642)
                     /locus_tag="ACHK0Z_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             complement(4427..4642)
                     /locus_tag="ACHK0Z_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504164.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            complement(4775..5053)
                     /locus_tag="ACHK0Z_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             complement(4775..5053)
                     /locus_tag="ACHK0Z_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504165.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            complement(5124..5414)
                     /locus_tag="ACHK0Z_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             complement(5124..5414)
                     /locus_tag="ACHK0Z_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU4504166.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(5411..6112)
                     /locus_tag="ACHK0Z_RS21225"
                     /old_locus_tag="pgaptmp_004245"
     CDS             complement(5411..6112)
                     /locus_tag="ACHK0Z_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4504167.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(6109..7047)
                     /locus_tag="ACHK0Z_RS21230"
                     /old_locus_tag="pgaptmp_004246"
     CDS             complement(6109..7047)
                     /locus_tag="ACHK0Z_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4504168.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRD
                     KWTQLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            complement(7080..7376)
                     /locus_tag="ACHK0Z_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             complement(7080..7376)
                     /locus_tag="ACHK0Z_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU4504169.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGITKKKSPVAAGDSKQIDMQF"
     gene            complement(7515..7748)
                     /locus_tag="ACHK0Z_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             complement(7515..7748)
                     /locus_tag="ACHK0Z_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4504170.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            7862..8566
                     /locus_tag="ACHK0Z_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             7862..8566
                     /locus_tag="ACHK0Z_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4504171.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            8627..8968
                     /locus_tag="ACHK0Z_RS21250"
                     /old_locus_tag="pgaptmp_004250"
     CDS             8627..8968
                     /locus_tag="ACHK0Z_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HGU4504172.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRANPDCCFFG"
     gene            9036..9497
                     /locus_tag="ACHK0Z_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     CDS             9036..9497
                     /locus_tag="ACHK0Z_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504173.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTTETNEAVLAALSAEVRGC"
     gene            9491..>9747
                     /locus_tag="ACHK0Z_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             9491..>9747
                     /locus_tag="ACHK0Z_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HGU4504174.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQYAAQCESNIYIA"
BASE COUNT         2349 a         2413 c         2195 g         2790 t
ORIGIN      
        1 agcagcgtgg caatgtaacc actcttatca tgatatgcag atttttacga tcgtaaacta
       61 ttttttcgct gataaaatac agaggttctc cctcccggca attcacgctc aacataccga
      121 tccatctcaa gcctcactcc cagcatcatc agcatgcctt caacaatccc ctccgctttg
      181 tgaaggcgtt tacctataca ggtgtcagag cacccatgtt tccgtgccag cgccatgaac
      241 gtctccccca acacgtaata atcaaccagc aagtcatgca gatcgctgtt gttcctgtta
      301 aggcgagcca tacacccgca tataatcatc gcgtcatcgt cacaacactg tggacgtgat
      361 tttacttttt cggggatcag tcccttaaat ccggcagcaa tgggcgacca tgtaacatcc
      421 tcatggttat ttgccgccca tgccccccag cgctcaagaa cctgccggat atcacgcatc
      481 agtatcttta ccccatccgc gatgaaccat aaggacgcca ttgacgacgg cgtgcttttt
      541 cccttcttta tcgccaatgt attttctgac tgtggcacga ttgcagttca gtattcgggc
      601 tacctcggtc tgatttccat atgcctcaag gagcatgtca ggaatggttt ttactgtgaa
      661 cgtcatgcgg cctcacttct gctgtttcgc aggtctttaa gtttctgctg atacttcgcc
      721 ttgatcgccc tgcattcttc gacagtccag cgatggcggt tatggttcga ttcgatttcg
      781 tctactgctt cctgcccgat gcggttaatc agttcgacgc gatacggaac gagatttccg
      841 cttttgtgct ggttgcacac cacgcattgc ttgtgaatat tgcgttcatc aaatcggagt
      901 tgaggtgtcg cagcagttgt ccggtaatgt ccggcatccc actgagcaga cgtgagcgtt
      961 ccgcacgaga tacatggtaa gtcgcggtct ctttctctga tgaaggcgtt tacggcttgt
     1021 tgggcttgtt taatccagta actgcggggc tttaaggcga gttttcgaat cttcagttta
     1081 tctttctgtt tctgctcctc tcgtcgtcgt ttcttctctg ctgctttttc cgctttttcg
     1141 cgttctttac ttcgtcgttc gagtgctaat tgagttccgt gttccgggca gcaccaccac
     1201 tgatttgaga atgccgggtg aaaccattcc ttgcatattt tgcatttcct tcgcgctggt
     1261 ttagccatta agcagcctcc cttgttactt tcagcattcc gttatcgagc agctttctgg
     1321 tcagccactg ttgaccacgc ccggtgattt ttgtggtgaa cgatatctgt attccgtgat
     1381 ttgtattgac cgctgtttct ttcactgtga aatagccacg atccatatat tcctgcattg
     1441 gcacattgcg ccgggagcct gaagcaataa ggattttgtg atcgcgcatc cacgcaaaca
     1501 gtttgtttgg accaatacca acaacctttg caaagtttcc aatcaaaatt ccgctggcct
     1561 cgccaacgcg atcggcaaac tcaactttag gtgctgcgag agcaagctgt ttctccagtt
     1621 cagccttctg gtcttcaagg tcggccgcaa ggcgcaatgc ctcagaaaag gtttgtggta
     1681 ttttcgcggt tgccccttcg agttctcgcc agcggtcaac aaggcgagcg gtgaattccg
     1741 gcgacaactg ggcaacgaca ataatgctgt cgcgcttacc ttgttcgccc tcaaaaacgt
     1801 aagcctctac gccacgaagt aatcctaagt tattgatttt ttcgaaaacc accattgggg
     1861 gatttcggat cacacctcga accgccagtc gttcaatgga ttgtttcacc ttgtcatgac
     1921 ggcttcccac caactcagcg atttcaatgc ttgtcatttt gatggcattg ctatttatca
     1981 gctcattcat tgtcatgtcc tctcacattg aaaattcagc aataaaaaac ccagccgaag
     2041 ctgggtttgt taagttgtca attgtcagta gcgatgcagt gaaggcggca actctttgtt
     2101 cttaagcctt tcccatgcca gaaggttcgt cggcccgtca ggctcataaa tatctatatc
     2161 ccgcgtgtga ttaattaaaa cgcccctcgc cctcccgatg atatacgaga actcatagcc
     2221 gtagtcgtgg catatgccgg aatagccaga ctgaatcagt tttaatgcgg gatacaactc
     2281 acggaacaat gcctgtgagc ggttggcata atcccacagc catacaaggc tgtctgtttc
     2341 ttttgcggaa agcccgttga gcttcttctc ttgtttgcca gtatttttct cgcactggct
     2401 gaaatagcag tcttccagtt tttctaacac atcccacgcc tgatcagttt cgagcatttt
     2461 tgcgtgacgg gctgcgcctc gttctgtcca gagtatgagg gagcgggctt tcggggaaat
     2521 ttgtaaccct cttaaagatg gttgcaaatt ttgtgagtta cttaaagtaa cccgcaaatt
     2581 ttgtgagtag tttaaagcta cccgcaattc tttaaggtca ttaccaacaa ctttgaaaaa
     2641 gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa tacgcaccgg
     2701 ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acaggaatct ggttatgggt
     2761 gatcggggag agagtttcaa cagaaatttg agttgtcata atgacgccct ctggtggttt
     2821 cttaataact caccaccgac gacgccaatc gtctggtggt gaactgtgca gggttggcgt
     2881 aaccgggaaa ccgaccggcg cggatctctc cgcccccaca cagcccacca taattcagat
     2941 gtgcgcgtgc atacgacaat aaaaaacacg ctcgcggcgt gtatctgtcg cggtctctat
     3001 ccaggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag ccccgaataa
     3061 atcatcgcgt caatcacctt gttttcctcg cacgatgtct tagccaccgg atatcccaca
     3121 ggtgagccgt gtaattgaag gtttttacgt cagattcttt tgggattggc ttgcgtttat
     3181 ttctggagcg tttcgttgga aggtatttgc agttttcgca gatgatgtcg gtgatacttc
     3241 gtcgctgtcg cctcatgccg ccctcctgac gccctgcccg atcgccatca atgccgcttt
     3301 ggatacagta gtaaacattc gtcgaggact gatgaacggt cgccaaatca gcagcatgga
     3361 gcctttgctg tttcccttct tctccagccc tgtcgatggt tcgataaaat taatccgtcc
     3421 atcagtgatg atgcgaactt cgtcaacact ctccagagcc ttgctgaacc atccgactga
     3481 catatcctct ggcacaagca taactaccgt ctgtcgctgt tgtatgcact gctcagcggc
     3541 tttttccacc cacggcctga tattgctgta cggtgggtta ttccagattg caccgtggct
     3601 tacccactca gaattgagcg cgtcgtcggc ctcagttagc cagtgagcgc acagagcatt
     3661 tttgtcgctc gctgccgaat ccagccagaa tccaaactca atatccagtg catcaaaaag
     3721 ccaaagcggc gtttgccagc agtccttgtc gtgtgctggc gtatttgatt tgatagtcat
     3781 gcagccctac cttttcgttg tgaccattca tactctcgcc gggagtcatc actccaccgc
     3841 acgttgcgct ctgagccgaa ccagaacatg atttcgataa gctcagtcat gctggccttc
     3901 cgcattttgc tggtacgcac gccaagcatg acaacgccac cgtcgatacc aggcacactt
     3961 cgttgctcca gttttttggt cttaagccac agggcagtga acaggtcttt ccagtcttcc
     4021 ggcgccagcc gttgaccatg ccatagcacc tgacgcgaaa catcgttcag catcggccac
     4081 atgcggtcat tctgcgcttt gctgcgcttg ggttctttaa cgtggacttc gtggggtgac
     4141 ttgtcgtcga tgggtagtga gagaatggcg tctatggcgt tatttctgat tgcttcgttg
     4201 cgaagcagaa aggtttgctt catctcctgc tctccggttc catttttcag ccgccgcagc
     4261 aactgatggt gcccatgccc ccctggcttc acagaggtca cattctgcat agccccacac
     4321 atcaatattt attccggcct caacccacag acgagcatta ccgccgcaaa acggacattc
     4381 ttttagcttt ggctgggtta atgataggtc gctcatgctc actccttcac tttaaatcca
     4441 gactccggat aattctgttg cgctgaaact cattgttgag tttgaacaac cgtcgaagaa
     4501 cacggtcacg cggatagcgt cgtgcggcag gtgaatgctc atacaactca tcaagcggca
     4561 aactggacga tgaacgatac cgataccaac gcaccaactc ctcacgaaaa ttagccctga
     4621 caagctcagc tatcgtactc atttcttaaa acctcctcaa acgcattctg acgcattttt
     4681 cattctcgct gcttattggc atgccttgca cgcgtttacc tcgctacaga gcgattgtga
     4741 tgccttaaaa gcgatttatt gaagtgatat ttgcttaatc gaaattcttt tctttgattc
     4801 ctgcggccct gatggctttc attactgcaa ttaccgtttt gtcacgccca tcctcataac
     4861 ccatcgcata agcaccttct tcaccatctt tccaaaagtc gtcattcgat tcgggccagt
     4921 cgatatccag ttcaatagca gagcgcgatg cctgccatat cacccaggca aactctttta
     4981 attcatcgtc tcccgtgaac tggcttttgt cttttgacca ccagttttca aactgtcggt
     5041 agctatcgtt cacttccctc tcccccaaat aaaaaggcct gcgattacca gcaggcctgt
     5101 tattagctca gtgatgtaga tggtcatctt ttaactccat ataccgccaa tacccgtttc
     5161 atcgctgcac tctggcgaca ctccttaaaa atcaggttcg tgctcatctt tccttcccgt
     5221 tcttccctgg tagcaaaccg gtaatacacc gttcgccaga ccttaccatc aatgaccagg
     5281 attcctgccc gcgccatttt agccgcagcc tgatttatgc tggttacggt tgcgcctgtt
     5341 accgcggaaa cgtcctgtgc acagaagttc ttatgcgtcc cgaggtaatg aataattgcc
     5401 tctttgcccg tcatacactt gctcctttca gtccgaactt agctttaatt tctgcgatct
     5461 tcgccagcgc ctgaacacga tttagaggtc tgccgcccat gacaggaagt tgttttactg
     5521 gttcaggtat cgtctcacca cggttaattc gtgctgtcat acaggtcagt tcatcggcag
     5581 ccttgcgccg taattccgcg tcagtcagcg cattggcccg catgttctgg tacaggttgg
     5641 taaccagcca gtagtgcgcg tttgatttcc acggataaga ctctgcatcc ggatacaggc
     5701 cacgcttccg gcaatactcg taaaccatat caaccagctc gctgacgttt ggcagtccgg
     5761 cgataacgga tgcttcttcc cggcaccatg caacaaactg cccgggtgat ggaagaaatg
     5821 gtcgattctg ccgacgggct acgcgcattc ctgcgttaac ctgttccatc gaggtgatcc
     5881 cgttttcccg gaaagccaga acccactggc ggcggatttc attcagttcg ttctggtccc
     5941 ggttagccag actcgccggg aaagttgcca gtaactggct gaacacaccg ttgatgatct
     6001 gcgctacctg ttgtacctgc ggcttttcgt cgtactgttc cggcatgttg ttggcgattc
     6061 gacgcatctg ctcacggtca aagttaacca tctgtgcggc gatgtttttc ataaatccac
     6121 cccgtaaatc cagtcagtgt ttgtcaggtc gagttttggt ttgctggctg tcacgcctgc
     6181 ctgttgcttg ttacggttga tttcgagctg ggtccacttg tcgcggagtt tggccggact
     6241 cagcacgtta ccggaccaga agttgtcctg gcaggcccag cggaaaagca cacacatatc
     6301 gcggtggtta cgtccgtcac gttcacgcat caggcggata tcgttagccc acccagcaaa
     6361 attcggtttt ctggctgatg gcgcgatagt cttcaccatg tcaaacatcc actctgcggc
     6421 ggtcaggtct tctgctgttc cccacttgct gccgctctga attgcagcat ccggtttaac
     6481 cacagaaaga tcgttttctg gctggtcaga ggattcgcca gaattctcgg acgaataatc
     6541 ttttcttttt tcttttgtaa tagtgtcttt tgtgtccccc tgttttgagg gatagcaatc
     6601 ccctaatttg agggatgttt tatccctcgt tttaggggat tttccctcgt tttgagggat
     6661 gtccctcatt ttaggggaac ctccctcgtt ttgagggatg caccattctg agatgttttt
     6721 atttggtcca aacatgccgc cttgctgctt gataatattc attctgacga gttctaactt
     6781 ggcttcattg caccgtttga caggtaactt tgtaatctcg ctaagttgag aatcggtgat
     6841 tctgtccatt ggtttattcc acccataggt tttacgcaga atggcaagca gcactttaaa
     6901 ctgtcgcttg gtcagatctg cgcctgaata agcctcaatc agcatatttg atagtctggc
     6961 gtaaccatca tcgagatctg ccacattacg ctcctgttcg gcaaagttac ctctgccgaa
     7021 gttgagtatt tttgctgtat ttgtcataat gactcctgtg gattgatcca gtaattccct
     7081 cagaattgca tatcaatttg cttagagtcc ccggcggcca ccggggattt tttctttgtg
     7141 attccatcaa gcgcatactt aaaagcccta ctaatcggac tgatgtctga tgccattccg
     7201 aaagcacaca agaccgaagc aataaatctc cagtccgttc tgcttatctt cgattcatga
     7261 cagccaatca tctttgccag accgcgctgg gtaagcgttg acaggttgat gagtaaatca
     7321 gtttcagcgc gatcaatttc tcgctgtgat agtttgctgt aacttgtttg ttccatttct
     7381 taagatttcc aatagtgaat agttagttga aaggtatgcg tggaaacgca tgtggcctta
     7441 gttggtcaga tatattggga ctcgctttgt cagcgacgta ggacgaatgt ccattgtgaa
     7501 aatagcggtg ttacttatgc agccgatgct ctacgcgata cgaacactag gttttccttt
     7561 ttcacaggtt tataacccgt gaaattacga gtagcttctt cgattgcatt cgctttatca
     7621 ggggaagctc ttcgaaatcc atatgcaatc tggtcaagat agccaactga agttttcgct
     7681 aatgcggcga gtcgcttcca ttcctcacta gaagcctctt ttcgccagcg tagtagttca
     7741 ttactcatta gtgcctccgt ttatcacaca gaacaaattt accattttga taaatcatcc
     7801 gcaatgtaaa tttatcatat tgcgtattta tccatttgct aaatagaggg aaaattgtga
     7861 gatggaaaac aaagatattc gcaaatcgaa tctggcgttt ttgctagatg agcataaaaa
     7921 aatcgcgggt aacactaatg caagctttgc cgataagctt ggggttagcc cttctcaact
     7981 cacgcaagtc tccggtgaaa aaagcactcg aaacataggg gataaactag caagaaaatt
     8041 tgaagccgcg cttgggttac ctaatgggtg gcttgatttg gtacatgatg taacaccaat
     8101 tgcatcatgc tcagattctt taacttttgt cggtcaggta agaaaagggt tagtgcgcgt
     8161 ggttggtgag gcaattcttg gtgttgatgg tgccatcgag atgaccgaag agcgcgatgg
     8221 gtggctcaaa atttatagcg atgatccaga tgcctttggt cttcgtgtga aaggagacag
     8281 catgtggccc agaataaaat caggagaata tgtactcatt gagcctaaca ccaaagtatt
     8341 cccgggtgat gaggtgtttg tcagaaccgt tgaaggacac aacatgatta aggttcttgg
     8401 ctatgacaga gatggagaat accaatttac aagcattaac caggatcaca ggcctataac
     8461 gttgccttat catcaagtag caaaggtgga gtatgtagct ggtattctga agcaatctcg
     8521 ccatctggat gacatcgagg caagggagtg gctgaaaagt tcgtgacttc atcgtcacat
     8581 agctggtaac cagtggcctg aagagacgtt tgggtaagga ggatagatgg cgttcaatga
     8641 ccttgaatat caagcagtaa aaaaagaagt tcaccaattc attgaaagca taaggccgcc
     8701 tgaacatatc cgcaatgaac tggatattgt ttatagcatc aatgaccaaa cgatagatat
     8761 cggcgaacag cgccccgtgt ggcagggcaa tccaggtgaa acaaacatcc tgccatcagc
     8821 aagaatcaag tacatacgtt ctctggatag atggaaaatc tattggatgc ggaaggatat
     8881 gaaatggcat cagtacagta ctgaactttc gctgactgat gcgcttgagc ttgtgcgtgc
     8941 taacccggat tgctgcttct tcggatgagt gaagagacgt ttggatgatg gatggtcgca
     9001 gagatgcggc ctgattctaa aataggatat aaaaaatgag aatactaggt gttagagcgg
     9061 cgcccaaagt tacatctttt gttgtatatt gcactaatga gtctgcactc aaatgtgttg
     9121 atgttattaa aataccttcg accttagaca caccagaaaa attaaagtat gtgagaaata
     9181 acatcctcga cattcttaat ttatataatg ttgaattagc tgccatacgc gttactgaat
     9241 caaactctga taatcttagc attgaccgcc tttatataga agctgttatt caagaagcat
     9301 tttcaagcag tgatgtaaga aaatattaca ctattagaaa atctggcatg aaatcatcat
     9361 tgaacctaac agagatcgag tataaagaaa tattgaagtc acaccgcaat ataaatggaa
     9421 tcgataattc tggttttaca actgaaacaa atgaagctgt tttggctgca ctatctgcgg
     9481 aggtaagggg atgctaactc catacaaaag agctgatgta gaattcgaat ggattagtga
     9541 tctagaagaa cagggttgtt tttcaaaagt atatctggct catgacagac acctagctca
     9601 tgacttggtg attaaagaaa tagaaaaaaa agaaaacact aaccacgacg actactttaa
     9661 tgaagcaagg cttctctata aacatgcaca tccaaatatt gtgcaagttc agtatgctgc
     9721 tcaatgtgag agcaatatct atatagc
//
LOCUS       DAZFWP020000176         9611 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFWP020000176 DAZFWP020000000
VERSION     DAZFWP020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.176"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            107..721
                     /gene="evgA"
                     /locus_tag="ACHK0Z_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     CDS             107..721
                     /gene="evgA"
                     /locus_tag="ACHK0Z_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU4504175.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            726..4319
                     /gene="evgS"
                     /locus_tag="ACHK0Z_RS21270"
                     /old_locus_tag="pgaptmp_004254"
     CDS             726..4319
                     /gene="evgS"
                     /locus_tag="ACHK0Z_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU4504176.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4375..5520)
                     /gene="yfdE"
                     /locus_tag="ACHK0Z_RS21275"
                     /old_locus_tag="pgaptmp_004255"
     CDS             complement(4375..5520)
                     /gene="yfdE"
                     /locus_tag="ACHK0Z_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU4504177.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5594..6538)
                     /gene="yfdV"
                     /locus_tag="ACHK0Z_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     CDS             complement(5594..6538)
                     /gene="yfdV"
                     /locus_tag="ACHK0Z_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU4504178.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6608..8302)
                     /gene="oxc"
                     /locus_tag="ACHK0Z_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             complement(6608..8302)
                     /gene="oxc"
                     /locus_tag="ACHK0Z_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU4504179.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8356..9606)
                     /gene="frc"
                     /locus_tag="ACHK0Z_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             complement(8356..9606)
                     /gene="frc"
                     /locus_tag="ACHK0Z_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU4504180.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2845 a         2190 c         1992 g         2584 t
ORIGIN      
        1 aaaggaagct cagattttct tatttttatt gagaaaatga gatgacgcct tatgtctgta
       61 ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat
      121 tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat
      181 tgaaatctta gcggagttga ctgaaggggg aagcgccgtt cagcgggtgg aaacacttaa
      241 gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga
      301 aacgctgagg aagcgccaat atagcggaat tattattatt gtctccgcta aaaatgacca
      361 tttttacggg aaacattgtg ctgatgctgg cgctaatgga ttcgtgagta aaaaagaagg
      421 catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt
      481 ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc
      541 gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc
      601 tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc tgatggaaaa
      661 attagaatgt aaatcactga tggatcttta cacattcgcg caacgtaaca aaatcggcta
      721 atcacatgaa gtttttaccc tatttttttc ttctctgttg tggtctttgg tcgaccataa
      781 gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac
      841 ttgatccagt acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg
      901 tgattgcagt acacaagtcc caaacggcta ccttgttgca taccgattcg cagcaacagg
      961 ttcgtggtat taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa
     1021 cactccggga atacgccgat catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg
     1081 atatagtgtt atcacattta gttacttcgc cgcctcttaa tagtgacatt gctgcaacca
     1141 aaccattgat aattaccttt ccggcgctgg taaccaccct tcacgactca atgcgaccgc
     1201 ttacctcacc aaaaccagta aatattgctc gggtagcaaa ttacccccca gacgaggtaa
     1261 ttcatcaatc gtttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag
     1321 catccgtatc agccggacag aatgattact ttattggtag taacatcatt accagcagta
     1381 tgatttcccg ctatttcact cactccttaa atgtagtgaa atattataac tcgccgcgtc
     1441 aatataattt tttcttgacc agaaaagaat ccgtcattct taatgaagta ctcaatagat
     1501 ttgttgatgc tttaacaaat gaagttcgct atgaagtttc acaaaattgg cttgatacag
     1561 gaaacctggc ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta
     1621 agcaacatcc cgatttaaag gtgctggaaa atccttactc tcctccctat tctatgacgg
     1681 atgaaaatgg ctcggttcgg ggcgttatgg gggatattct taatattatt accttgcaaa
     1741 caggtttaaa tttttctccg atcaccgttt cacacaatat ccatgccgga acacagctta
     1801 accccggtgg atgggatata atacctggcg ctatttatag tgaagatcga gaaaataatg
     1861 tttcatttgc agaagctttc ataacaacgc catacgtttt tgtcatgcaa aaagcacctg
     1921 acagtgaaca aacattaaaa aaaggaatga aagttgccat tccatattat tatgagctgc
     1981 attcgcaatt aaaagagatg tatccggagg ttgaatggat acaggtcgat aatgccagcg
     2041 ctgcatttca caaggttaag gaaggtgaac ttgatgctct ggtcgcgaca cagttaaatt
     2101 cgcgttacat gatcgatcat tactatccta atgaacttta tcattttctt attcctggcg
     2161 ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta
     2221 ttaataaagc actgaatgca attcccccaa gcgaagttct gcgcctgacg gaaaaatgga
     2281 ttaaaatgcc caatgtgacc attgacacat gggacctata tagcgagcaa ttttatattg
     2341 ttacgacatt atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac
     2401 gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata tcattccgaa
     2461 aagcactctc ggattcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca
     2521 ttagtcataa tagtgctttt gaacattatt tcactgcgga ttactacaaa aatgcaatgt
     2581 taccattaga aaacagtgac tcacccttta aagatgtttt ttctaatgcg catgaagtca
     2641 cagcagagac gaaagaaaat cgaacaatat acacacaggt atttgaaatt gataatggca
     2701 tcgagaaaag atgcattaat cactggcata cattatgcaa tcttcctgca agtgacaatg
     2761 cagtatatat ttgtggttgg caagatatta ctgaaacgcg tgatctaatt aatgcactcg
     2821 aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga
     2881 tgagtcacga aataagaaca ccaataagct ctattatggg cttcctggaa cttctgtcgg
     2941 gttctggtct tagcaaggag caacgggtgg aggcgatttc acttgcctac gccaccggac
     3001 aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact
     3061 atcaacttca accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt
     3121 tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg cagcagtaca tttcctgatc
     3181 attacctggt taagatcgac cctcaggcgt ttaagcaggt cttatcaaat ttactgagca
     3241 atgctctcaa atttaccacc gagggggcag taaaaattac gacctccctg gttcacattg
     3301 atgacaacca cgctgttatc aaaatgacga ttatggattc tggaagtgga ttatcgcagg
     3361 aagaacaaca acaactcttt aaacgctaca gccaaacaag tgcaggtcgc cagcaaacag
     3421 gatctggctt aggcttaatg atctgcaaag aattaattaa aaatatgcag ggcgatttgt
     3481 cattagaaag tcatccaggc ataggaacaa catttacgat cacaatcccg gtagaaatta
     3541 gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc catcacacta cctgaaaagt
     3601 tgagcatatt aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa
     3661 atctattagg atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca
     3721 gtatgcaaca ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg
     3781 agttgactcg caaactccgt gagcaaaatt cttccttacc catctggggg cttacagcca
     3841 acgcacaggc taacgaacgt gaaaaagggt taagttgcgg catgaactta tgtttgttca
     3901 aaccgttgac cctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata
     3961 ttgcacctca gtatcgccac cttgatatcg aagccctgaa aaataatacg gcgaacgatc
     4021 tacaactgat gcaggagatt ctcatgactt tccagcatga aacgcataaa gatctacccg
     4081 ctgcgtttca agcactagaa gctggcgata acagaacttt ccatcagtgt attcatcgca
     4141 tccacggtgc ggctaacatc ctgaatttgc aaaagttgat taatattagc catcagttag
     4201 aaataacacc tgtttcagat gacagtaagc ctgaaattct tcagttgctg aactctgtaa
     4261 aagaacacat tgcagagctg gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa
     4321 tagcggctcc cacaatgttc aaatgtggga gctatttacc agcacatctt acatttatga
     4381 tgagaactcc tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac
     4441 atgcgggtcc gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcccccggc
     4501 ttcaatcaac atatttctcg cctgagtttg tggcaattta atggcctcag ccacacttaa
     4561 taacggcgcg acgggtacac caacttcatg tattctggct aaccaaactt cagctgcctg
     4621 cgtttttaac gtccgctcaa tatattgttt aagaatagcc tggttttgta cgcgtaaaat
     4681 attgctgcta aatcggggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc
     4741 agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tattgaaaac
     4801 atcaaaaggt gccatgtagg gatggcgatt tcccagacgt tgtggtgact tcccagttgc
     4861 gatatatgcc atcagaccat gctccagaaa actcagcgtg gcatcaaaca tcgctatatc
     4921 gacatgcgcc cctctctggc tcttttcgcg gccataaagt gcactcacta ttccgctgaa
     4981 taaatagaca ccgccgcata gatccgcaag agaggtacca acgcgcactg gcggagcatc
     5041 aggatatcct gtttccatca ttatcccgct cattgcctga atgatggtat cgtaggcagg
     5101 agcatctttt agcggaccgg tatgtccgaa acctgacgat gaagcatata tgaggcgcgg
     5161 gttgatttct tgaagcgttt cccatgaaaa ccccagtttt tccattgtac ctgggcgaaa
     5221 attctcagct aatacatcag cttgtttgag catatttata aatatacttt tatcgtgatc
     5281 attctttaaa tcaagaacca cactctcttt gccatgatta ataaaactgt aatagagtga
     5341 ctgtccatcc acatagggac caaatgtgcg ggtatcatca ccatgacccg gcggctcaac
     5401 tttaattacc cttgcgccca tattacaaag aagttgagtt ccgaaaggtc cattaaggac
     5461 atgtgtcata tcgataacta ataagccttc aaacggccct ttgctttcat tatttgtcat
     5521 ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact tcggcattta tgtaaaatca
     5581 ataacatgat taattatgaa accagtcgac tgacataaat ccacaaggga gccgtgacga
     5641 caaagcccag tacgctcaca gccaatgacg ctgtaccagt acgggtgtag acattaaacc
     5701 ggctggcaat aatgattccg gagaatgccg gcggtaatgc gcctgccagt accatcatct
     5761 gcagatgttc gctgttcaaa tgacatgcca taccaacgag aagcagtgcc agtggcatca
     5821 gaatcagctt caggaaggtg ttataagcaa tttcggcact gaactcgaat ttatgtgcag
     5881 ccagagtcaa ccccgcagcg aataccgcta cccctgagtt agccttcgca atcagattaa
     5941 aggttgggtc ccatgctgcc ggaattttta ccccaaccaa caccaggatc gttgccagaa
     6001 caggtgccca tactactggc tcctttgccg cagaaattaa tgcgctcaga ttactattct
     6061 tcttaccatc cgctcctgag gaaggattca gcaaatacag accaatagga atagtaattg
     6121 cgttaacaat aatagaaata attgctacca ctaaacctgt tgatacggaa tcaccataaa
     6181 taggatcgag aactgcaaac cccaggaatc caatggtagg tgaacccgca attaatgcac
     6241 atacagctgc ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa
     6301 agaaacatcc gacaataacc acaagtgata caagggtcag acgagtgtcc gcaaaaatca
     6361 tttccctgtt tgcccgggta atagaaacaa atagagccgc aggaagagcg tagtttaata
     6421 ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat
     6481 aacccaacag catgattacg ataataggca ataaatcgcc aataaaaaat gttagcataa
     6541 tagtatttcc gataagggtc ccctcaccat cgcataatga gttggcaaga ggagacgatg
     6601 tcagtattta attaccagcg acttgttttg ggttaagttt ggtaatatgg ccactttcag
     6661 ttcctgctgc agggtcgatg accacattaa taatggtcgg tttgcgcgac tgaataccgg
     6721 tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg
     6781 catccattaa tttgtcatac cttgcatggt gcaacagatc cgttggtgat ggtgcaccag
     6841 cgccactgag atcaacaccg tctcctctgt agatgccgcc attattaaaa ataacgatcg
     6901 tcaccggcag gttatatcga caaatcgttt caatttccat cccactgaaa ccaaaagcac
     6961 tatcaccttc aatggcgaca accggagaac cagaagtcac gctagcaccg atggcatagc
     7021 ccataccgat gcccatgaca ccccaggtac cacaatccag acgacgacgt ggtttataca
     7081 tatcaataat atttcgtgca ttatccaggg tatttgcacc ttcattaact aaataaatat
     7141 cctggttctc gcgcaatacg tcgcgcacag cacttaatgc attaaagtaa tttaatggtt
     7201 gagtatctgt acttaatttt tcatgcattt tttgtgcatt ttgctgcttg tggatattta
     7261 aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg tttcagttct gccagcatac
     7321 cttgcatact ggatgcaata tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa
     7381 tttcctgcgg ttcaatatcc agttgaataa actgtgtatc tgccgcccat ccttttttac
     7441 cgtgtgccaa taaccaattc agtcgtgcac caacaagcat gacaacgtca gcatttgcca
     7501 gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt atcttcaagg atccctttcg
     7561 ccatagacat tggcaggaat ggaatctgag tactttcaat aaattcacga agctgttcat
     7621 cagcttgtga atacgccgcg cctttgccaa ggataattaa tggccgttca gcttttgcta
     7681 aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa taatgctggc gacggatttt
     7741 caactttaac aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc aggacatttg
     7801 ctggcaaatc aagataaact ccgccagggc gacccgatac agagacccgg atagcgcgtg
     7861 ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa tgctgctttg gcatacggtt
     7921 ttgccgcatt catttggtcc agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc
     7981 ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt taccgttgcg ttggccaatg
     8041 cggtcaaacc attgaggaat cctggcgcag aaactgtcag gcagatcccc ggtttttggg
     8101 taagaaaacc gcttgccgca gcggcatagc ctgccgactg ctcatgacga aaaccaatat
     8161 aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt cacaggaata cctacaacac
     8221 cataaatagt gtcaatatta ttctgtttta atgcttcaac gatgatatgc ataccatctg
     8281 tcatttgaag ttgatctgac atgattaata acctcgaaat ttttataaat tgggagccag
     8341 aagcccccgt taatatcaga tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct
     8401 ataacccagc tcctgcaata cagcagcggt atgttcacct aatagcggcg cagctttaat
     8461 atccggcgta aaggcagaga atttcattgg acaaccaacg gtcagatatt ttccacgcaa
     8521 cggttgttcc acttcaacaa cactgccact ttggcgcaaa gagggatcaa gtgaaatttc
     8581 tttcatactt aaaaccggtg cacaaggaat atcaaactga gtcaaatagg ccaccgcttc
     8641 atgtttatca atagtgacag tgtatttttc gatttcagca aaaatatcga aaatatgtgg
     8701 ctgtcgtgca tgggctgtac tgtatgccgg atcggtaatc cattctggtt tgccgatggc
     8761 tttacaggtg ttttcccagt tttgctcctg aatagtgaaa taaatatagg cgttaggatc
     8821 ggtttcccag cctttacatt tcaggatcca gccaggctga ccgccaccac ctgcattacc
     8881 accgcgggga actgcatcac caaatgtacc attcggatac tgcgggtatt cttccagata
     8941 acccaattta tcgagacgct gctggtcacg taatttcacg cggcaaaggt tcaatacggc
     9001 atcctgcatt gacatggtga ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc
     9061 agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct
     9121 taccagcggc ggaccatccc aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc
     9181 aacgttttca taggctttta cattcacata aggcgaacac tcatcaaacc ctttgatcga
     9241 accaaaaatc agacgtggat tgatttcttg aatatgctcc caggtgaagc ccatgtgatc
     9301 aatggcccct ggatgaaagt tctcgactaa gatatcagct tcgcggatca gcttttccat
     9361 tacctctttg ccttccgctg ttttggtatt taactcaata gaacgtttgt tactgttaag
     9421 catggtgaag taaagcgcat cgatatcagg aatatctcgc agctggtgac gcgttacgtc
     9481 accaacgccg ggacgttcaa ttttaataac gtcagcgcca aaccaggcca gcatttgagt
     9541 acaagatggg ccagattgca caccggtgaa atcgagaact ttaattcctt gaagtggagt
     9601 tgacatagca a
//
LOCUS       DAZFWP020000177         9568 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFWP020000177 DAZFWP020000000
VERSION     DAZFWP020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.177"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(712..1242)
                     /gene="yodB"
                     /locus_tag="ACHK0Z_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             complement(712..1242)
                     /gene="yodB"
                     /locus_tag="ACHK0Z_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4504181.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1585..2235)
                     /gene="zinT"
                     /locus_tag="ACHK0Z_RS21300"
                     /old_locus_tag="pgaptmp_004260"
     CDS             complement(1585..2235)
                     /gene="zinT"
                     /locus_tag="ACHK0Z_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGU4504182.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2492..3127)
                     /gene="msrQ"
                     /locus_tag="ACHK0Z_RS21305"
                     /old_locus_tag="pgaptmp_004261"
     CDS             complement(2492..3127)
                     /gene="msrQ"
                     /locus_tag="ACHK0Z_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU4504183.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3128..4132)
                     /gene="msrP"
                     /locus_tag="ACHK0Z_RS21310"
                     /old_locus_tag="pgaptmp_004262"
     CDS             complement(3128..4132)
                     /gene="msrP"
                     /locus_tag="ACHK0Z_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU4504184.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4241..4654)
                     /gene="hiuH"
                     /locus_tag="ACHK0Z_RS21315"
                     /old_locus_tag="pgaptmp_004263"
     CDS             complement(4241..4654)
                     /gene="hiuH"
                     /locus_tag="ACHK0Z_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU4504185.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4787..5458
                     /gene="hprR"
                     /locus_tag="ACHK0Z_RS21320"
                     /old_locus_tag="pgaptmp_004264"
     CDS             4787..5458
                     /gene="hprR"
                     /locus_tag="ACHK0Z_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU4504186.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5458..6816
                     /gene="hprS"
                     /locus_tag="ACHK0Z_RS21325"
                     /old_locus_tag="pgaptmp_004265"
     CDS             5458..6816
                     /gene="hprS"
                     /locus_tag="ACHK0Z_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU4504187.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEVDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6923..7764)
                     /gene="hchA"
                     /locus_tag="ACHK0Z_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /pseudo
     CDS             complement(6923..7764)
                     /gene="hchA"
                     /locus_tag="ACHK0Z_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
     gene            complement(8357..9430)
                     /locus_tag="ACHK0Z_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             complement(8357..9430)
                     /locus_tag="ACHK0Z_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4504188.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARNGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         2668 a         1973 c         2172 g         2755 t
ORIGIN      
        1 tattgatttt tcgtgtatgt ttttaagaca tttattccaa gaaaaattta taaccttttg
       61 attttgcgat tcggtatcat cggtctcgaa aaccggagta ggggcaactc taccgggggt
      121 tcaaatcccc ctctctccgc cactttatca atgacttatc tcccgacttc ccgccttgct
      181 tttcctaaac agaacaatcg tagaatattc ttgaagggtt agatcgtcac tgttttctgt
      241 tcgatactgt gacattcagc acttgattcg ctatggatct gacaggaagc tttcgagcga
      301 aaatgtgcag ttattcagtc gttttcttat cgctcaccat tattctttta gacattcatc
      361 ctatgaaact gccgcaaagt tggtggtggg aactgaagtt gcgtagagaa ggggtcaata
      421 attgagtcaa aaaagtaacg cggccgtcag attaaagccg cgggacaaag tccataattt
      481 ccaagagttg ttgaatttcc cagctttttt gaaagccttt gtatcatact aacaagtaaa
      541 aaaggtttaa ataccaaaat taacctgtat ttttgataag tcttaccatt gtacgattta
      601 cacagtttct gctcctcctt gttgatagtg gaaagggtgc attgtgcact tgcgtaaaca
      661 tttatgacgc gttgatgtcc atggttcaca tttccttgtc aggggatcga cttatttgcg
      721 gcgaggcatc attcgtaaca gagtattatc tttctgaatg tagtgatgaa agagtgctgc
      781 gccagcgtga gctgcgatta aaaagtaacc tatatttgcc caggtttcgt gaatattttt
      841 tatcagtgct ttaatttcgc tgtttggggt aacaaaggga gacacattga aaccaaggaa
      901 actccacgat tttccactgt aagccatcaa agcaatcccc agcagaggaa gtgcaaggaa
      961 ggttatataa agcatgatgt gcattagcga agccgctttc atttgccagg caggtggcgg
     1021 tggcacaata gaaggatcac tataacggtg ttttataatc aggcgtgaaa acattaacac
     1081 ccaaacgaat atacccgcat tgtaatgtgt ttctcgcatc agcagataag tactactacc
     1141 tttaggaaac cagccacgga gttccatcgc ggcataggtt attgcaacga aaagcagcgt
     1201 tatccaatgt aaataaattt gagtttttga gaatcggttc atttttgtag ctcctgtaat
     1261 ttttacagtc aagctatccc acgaacctta aaaaatcctt atacagatgt ggttttaaga
     1321 atttgttatt taaaaaattg atagtgcaca ttgtaaccag ccaatataag caatggcgtg
     1381 agcacaggta tctttctctg ttggccgtat tgtgtatgga atccgttatt ggttaactca
     1441 atgcgggtta gcttattaat atacagacac gtcatagtga tgagcgtgtc tgtagctagc
     1501 ttgcgttcag tggcgtttgt ggatggacca gtggcagtta tgatgactta ctaaagcggc
     1561 atcgaggcgt tatcatgaga atactcaatg agacatcatt tcctcgacca cttcttcgct
     1621 actcaactga tatggataat acgttggcca gttttccatt tcatttaata aagattgctg
     1681 tgaatcatta cccataaaaa tgtgaaaatg agatgatttt cgtggtgcaa ttatatgatc
     1741 gctaaattgt atatatttag gggctttgct ttcaggatct ttacattcga ataagtaacg
     1801 aacgcctttc ttgcctgatt tataggtgag tattttgtat ccatcgtaat catatttaca
     1861 ggatgttgtt tcattatttc tatggaattc aacaatgccg tcctcaatgc caatcatctc
     1921 gatatctgtt gcataacctt tgtgataata atctttaatt tcagcaaatg ttttagtttt
     1981 atctgcatcc gctttcttct gaaagacggg gtcaagtttg ccactttgca gtaaaggata
     2041 aacggattgc cagactccat cccagtcact gagcgttcgg ttttgtacat tggcatcatc
     2101 aaaaacacca ttagcagctt tttgttcgac ctctgttaag ggtttgccgt gtgagtgatg
     2161 accatgcgaa aaggcaggag cgctaacaat aaagacacct aaagcaacag ccagtttgtg
     2221 aagacgaatc gccaaaacag tttcctccag agtcattgta tatgatgtgt aatgttatat
     2281 tgtaacatat ggcaaaatgc aacaactctt aggagatgag agcatagttt tctcaggtgt
     2341 aattatctat cttattgaaa taaaatgaat tatattgata atttgaggtt gatgacaaac
     2401 gaaacataac ctgatgtgat ctgcgtaggc cggatagggc gctcgcgccg catccggcag
     2461 ggataacgcg tactttgtcg gcaatatgaa gttatacaga taatttgttg tgaacctgct
     2521 tgcgtaaccg gttaaaaagg gaacgcaact tcttataccg taaggctaaa agcagtacag
     2581 ccagcccagc gtagatgagc ggttgcggtg aaataatctt cactgaccac agataatgta
     2641 ttggggccag gatcgcgaca agatagacga aattatgcaa ctgttgccag tgcttgccga
     2701 gttttcgctg catcgcctgg gttgaagtaa acgctaaagc aagcaaaata acccagctga
     2761 taattcctaa cgttaaataa ggccgggtaa ttaactcctt gcctaataac gccagattat
     2821 tcacgcccag ctccagcaat gcgtaactgg ttaaatgcag tgtcgcccag gcaaagcacc
     2881 ataagcctaa caggcggcga gtgcgtatca ataacggctg ttttgcgtag cgtgccagag
     2941 gtgtgattaa caaggtcgcc agcaaaaatt tcagtgcagt gcgaccggta aaatgctgaa
     3001 tatctttcac cggatcggca cccagtccac cgtgattgat cgcccagacc agccagagaa
     3061 acggcaacaa tccggcaaga tgcaggctaa ctttcagcca tgtcacctgt tttgctgtca
     3121 gacgcattca gaaattctcc cgcaaatcca ggccacgata cagcgatgcc acctggtcgg
     3181 cgtaaccatt aaacagtagc gttggctggc gctgaacatc gaggatgccg cctgaaccaa
     3241 taaatcgttc ggtagcctgt gaccagcgcg ggtgatcaac atgcggatta acgttggcgt
     3301 aaaaaccgta ttcgtcaggc gctgccagat tccaggtggt tggcggacgc tcgcgggtca
     3361 gcttaatact gactatcgat ttaatccctt taaagccata tttccacggt acaatcagtc
     3421 gtaccggcgc gccattttgt ggcggtaacg ccttgccata aacacccacg gtcatcagtg
     3481 tgagcggatg cattgcttcg tcgagacgca atccttcgac ataaggatat ttcagcccgc
     3541 cgccgataaa gcggtcctgc tggcctggca tttgttccgg tgcataaatt gtttcgaaag
     3601 cgacatactt cgcattgctg gtgggttcgg caagcgccag caatttgtgc agcggaaaac
     3661 caatccacgg caccaccatc gaccatgcct ccacgcagcg catacgataa atacgctctt
     3721 ccagcgggaa gcgacgggtt aaatcatcgt gatcgagggt caatggtttt gccacttcgc
     3781 cgctgatttt cagtgtccat ggatcggttt tcaggctacc agcattagcg gcgggatcgg
     3841 ctttatccag cccgaattca tagaagttgt tataaccgga gactttatct actggcgtca
     3901 gtggcaggtt attttgccag gcggcaggct tgctgaactc cagcggtttt ccggcggggg
     3961 cgggcgggcg atcgttccct ttaaaccagc taagcagatc ggcatgcgca gcgtgaggca
     4021 aagaaagtgc agctgcgctg atgcccagtg cttttaacac ctgtcgacgc ttcataaaga
     4081 ataccgactc ggccgtaaca tctgattctt ttaaaaattg attctttttc ataagccatc
     4141 acactttttt gtcgttagcc agcaagagcc gtttactgtt aatctgtcag ggagtgccct
     4201 ccctaacagt agcagagatc tgcttttaca tatttatgca ttaactgcca cgataggttg
     4261 aatacccata ttggctaagt aataaaggca catgataatg ctcgttcact ttattaatat
     4321 gaaattcaac ggggatctca gggaagaaac tttcaagatt ttgtttcttg aaatagtccc
     4381 cggttttaaa tacgacacgg taatcgccag tagtcgcagt ttgctcgggc cacagtgcct
     4441 taattcgtcc atccttatct gttttggcgg tattaagttg taaccagccg ttgtccgctt
     4501 tcttttcaag agtgactgtc acgtcggcag cgggttttcc ggtttgctgg ttcaaaatgt
     4561 gcacgctaag aatgttttgt tgtgcggcat aaaccaaaga aggtaatgaa aatgctgccg
     4621 ttactacgga gagtactaaa taacgcttta acatgtttat atccttgtca tgtgaatgag
     4681 ttttgaggat agcgaaaaag cggccagggt aaccttacag cagcattaca attttgtaat
     4741 gaaccaggct gtttctataa catatgattt atggcatatt attttcatga agattttact
     4801 tattgaagat aatcaaagga cccaggagtg ggtaacgcag gggctttccg aagcgggtta
     4861 tgtcattgat gccgtttctg atggcagaga tgggctttat cttgcgctga aggatgatta
     4921 tgcattgatc attctggata ttatgcttcc gggtatggat ggctggcaga tcttacaaac
     4981 gttaagaaca gcaaagcaaa cccctgttat ttgccttact gcaagggatt ctgtcgatga
     5041 cagagtcaga gggctggaca gtggagcaaa tgattatctg gtaaaacctt tttcattttc
     5101 tgagttgctg gcaagggttc gggcacaatt aaggcaacat cacgctttga attcaacatt
     5161 agaaatcagt ggcttaagaa tggactctgt aagtcaaagt gtgagcaggg acaatatcag
     5221 tattacactg acgcgcaagg agtttcagtt actttggcta ctggcctcca gagctggcga
     5281 aattataccc agaacggtta ttgcgagtga aatttgggga atcaactttg atagtgatac
     5341 caatacggtg gatgtcgcca ttcgcaggct ccgcgcaaaa gttgatgatc cttttcctga
     5401 aaagctaatc gccacaatcc gggggatggg ctattcattc gtagcggtaa aaaaataatg
     5461 aaaagattat ctataaccgt ccgtttaacc ttgcttttta tattgctgct gtctgttgct
     5521 ggcgctggaa ttgtctggac tctctataat ggcctggcaa gtgagttgaa atggcgcgat
     5581 gatacaacac tcattaaccg gacagcacag atcaagcagt tgttaattga tggggtaaat
     5641 ccagatacgt tacctgtata ctttaaccgg atgatggatg ttagtcagga tatcttgatc
     5701 attcatggtg atagcatcaa taaaattgtt aaccggacaa atgtcagtga tgacatgtta
     5761 aataacatac ctgctagtga gacaatcagc gcagctggca tttacagaag cattattaat
     5821 gatactgagg ttgatgcttt acgaattaat attgatgaag tttcgccatc attaacggtt
     5881 actgtggcta aattggcttc agccagacat aacatgcttg aacagtataa aattaatagc
     5941 attataattt gcattgtcgc cattgtactt tgctcagtat taagtccgct gttaatcaga
     6001 acgggattac gagagatcaa aaagttgagt ggtgtaacgg aagcgctgaa ttataacgat
     6061 agccgggagc ctgttgaggt tagcgcatta ccgagagaac taaaacctct tgggcaggcg
     6121 ttgaataaaa tgcatcaagc cttagtcaaa gattttgaac gcctaagtca atttgctgac
     6181 gatctcgctc atgaacttag aacgcccatt aatgcattac tgggtcagaa tcaggttacg
     6241 ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa ttgccggtaa cattgaagag
     6301 ctggaaaata tttcgcggtt aacagagaac atactgtttc ttgcccgggc agataaaaac
     6361 aatgttttgg tgaaactgga ctcgctttct ctcaataagg aagtcgaaaa tttgttggat
     6421 tatcttgaat acctttcaga cgagaaagag atttgcttta aggtcgagtg caatcagcaa
     6481 atctttgcgg ataaaatttt actgcaacga atgttatcga atcttattgt taatgccatt
     6541 agatattcac cagaaaaatc gcatattcat ataaccagtt ttcttgatac caacggctat
     6601 cttaatattg atgtcgccag tcctggaacg aaaattcatg agcctgaaaa actcttccgt
     6661 agattttggc ggggagataa ttcgcgtcat tccgtaggtc agggactagg cctttcttta
     6721 gtcaaagcga ttgccgaatt acatggggga agtgctacgt atcactatct caataagcat
     6781 aatgtgttcc ggattacgtt accgcaaaga aattaacata aggcttcagt acgccataaa
     6841 ttaaccgtga aaaaacgcag ccacgtcgtg agtactaacg cggctgcgat tgattagcgc
     6901 ttacattcaa acgtaacagg gattaacccg cgtaagctgc cagcatttcc tgcgccgcaa
     6961 cgcattcgct gcaaaaggac tgtcgccagt gagaacttta cggtccttat gtactcgccc
     7021 ggtgatgtcg tcattaataa tgttcatgcc cattttcttc agctcttcgc cgaagtacca
     7081 ggtgagatga cccggcatat agcctatctc tggcgtttgt ttgtctgcgg cgtctgggaa
     7141 tgcgcaaatg gaataaccat tcagtgggtt atcgccgtgg cgaagcgcca gaaaagccgc
     7201 cgggccgtgg caaagggaga taacaaaacg gtcatttttg attgcccact gcaaagcagc
     7261 agctacgtcc tggctttctg gtaaaccaat aagtgcgcca tgaccaccag gaacaaagat
     7321 tgctgcatat tcgctatcag cgttgaggct ggcgacaaca tccgcgagtt tcttcggatt
     7381 gcggaacaac gatttatgtt gctcaaagaa tggcattact ttttcatctt tgtgcggcat
     7441 agcccagtat tcaaacttgg tcatcagacc ggaaatggtc gccacttcga attcgaaacc
     7501 tgcagcatgg agatgataca acggcagcaa cgtttcaatc ggatggttac cggtcgagaa
     7561 cagttttccg ttatcggttg gcaaataacg ttcgtccgcg gcgatcacca gaattttatg
     7621 cttaccgcga tacggttttg gatagtccac gccatcaaga tcagagacgg gactggtata
     7681 ttggctaagc gaatattctg aagggaagaa tgcattatct tcagcaatat cgacctgcgg
     7741 atttttactt gtttgaacag tcatagtgta ttccttattg ttgcttagtt agggtagtca
     7801 ctatatttgc gactgagctg gtcgctattt ttaaccagtc tggaaaattg agtcaatata
     7861 tcgaaagcgg tagtcggcgg gtgtaagtat gtaatgatga tgctgcactg gtgttgaata
     7921 ttattcacgc caaattttcc ttattgagca acgtcgtcgc aagtggcctc aatgatttta
     7981 gaccattatc aaaggtacgg ggcggcgggg agagatttag tgctgtgaaa tattgtggta
     8041 ctgatgaata tttatatcgc ggattggtag agaaaagatt atttcatgca ctatttttgc
     8101 aaatatttct atatgggaag cgtgtttgtt acggggtaat tcatcagaat gtgcatttgg
     8161 ggtgttggct ggaagagtcg ctggtagatg ttattgtggc aggaggggca gggtaaggtg
     8221 ctgtatcgat atgctttggc aattgcgtgt gccgcagatg gagatgaaat gccaataaaa
     8281 aaagcctgag ttcgatgagg gacgaactca ggctagtgtt atgttttaca gtaacacgcc
     8341 gcggcgcgtt ggctgattag aactggtaga cgataccaac tgcaaactgg tcatcagttg
     8401 cggcatcggt aatcgctgca tcgtctttat ccagcaggtt aaatttgtat gctgtgtaga
     8461 cgttcatgtt cttattgaag tagtaccagg taccaacttc gatataattt acgcggtcag
     8521 catcaacacc tttatgtcca ttacgagcat aaagatcttt gcctttggaa tatacgtagc
     8581 ctaaagacgg acgtaaaccg aagtcgaact gatactgtac aacagcttca aagttttggg
     8641 ttttatttgc aaaatgatca ttagccaaaa cggtcatgtt gcgggtttca ccgtacatca
     8701 ttgctgcata gatattattt gcatcatact tggctgctaa tgaccacact tcagcgttat
     8761 caccgtagcc gtcaccagct tgttcattag tacgatcaga tttgctatat gcacctacaa
     8821 aaccaaaacc ttcgatgttg tagtctacag acaacgcata gccatcaccg ttttgtttag
     8881 taacgccacg attgctgttc ttaccttgat attgcacagc aaaattcaga ccatcaacca
     8941 gaccaaagaa atcagagtta cggtaggttg caacaccgtt ggtacgaccg ttcatgaagt
     9001 tgtcagcgga agcccaggaa tcaccacccc attcaactaa catatcggta taagctgcgg
     9061 catcataagc aacaccgtag ttacgaccat agtcgaaaga tcctaactcg ttatatttca
     9121 aacctgcgaa ggttaaacgg gttttttcct gattagagcc ttcgggcttg ctggcgtcga
     9181 ggttgtattc aaactgacca aaaccagtca gttcgctggt gatttgggtt tcacctttaa
     9241 caccgaaacg agcataggag gtatcttcgt tttcgctgtt attatcatca gtatttgacc
     9301 agatgcgttc gccaaccatc ttgccgtaga aatcaacttt attgccatct ttattgtaaa
     9361 tttcagccgc atttgctgcg ccagcaacta ataacgccgg gaccagcatt gccagaactt
     9421 ttcttttcat tatgtattcc cttgtgatta taatcttcat gaatatatca ataagtgccg
     9481 ttatccaaaa aaaagcacat ttggatacta ttctatgaag ttcattttat tttaaagatt
     9541 acatgtaaca aatgtattta aaagattt
//
LOCUS       DAZFWP020000178         9413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFWP020000178 DAZFWP020000000
VERSION     DAZFWP020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.178"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..196)
                     /locus_tag="ACHK0Z_RS21340"
                     /old_locus_tag="pgaptmp_004268"
     CDS             complement(<1..196)
                     /locus_tag="ACHK0Z_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504189.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYN"
     gene            274..1368
                     /gene="ddlA"
                     /locus_tag="ACHK0Z_RS21345"
                     /old_locus_tag="pgaptmp_004269"
     CDS             274..1368
                     /gene="ddlA"
                     /locus_tag="ACHK0Z_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4504190.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1392..1604)
                     /locus_tag="ACHK0Z_RS21350"
                     /old_locus_tag="pgaptmp_004270"
     CDS             complement(1392..1604)
                     /locus_tag="ACHK0Z_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU4504191.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1864..2172
                     /locus_tag="ACHK0Z_RS21355"
                     /old_locus_tag="pgaptmp_004271"
     CDS             1864..2172
                     /locus_tag="ACHK0Z_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU4504192.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2231..3325)
                     /gene="yaiW"
                     /locus_tag="ACHK0Z_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             complement(2231..3325)
                     /gene="yaiW"
                     /locus_tag="ACHK0Z_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU4504193.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3338..4558)
                     /gene="sbmA"
                     /locus_tag="ACHK0Z_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             complement(3338..4558)
                     /gene="sbmA"
                     /locus_tag="ACHK0Z_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU4504194.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGEWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4910..6067
                     /gene="ampH"
                     /locus_tag="ACHK0Z_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             4910..6067
                     /gene="ampH"
                     /locus_tag="ACHK0Z_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU4504195.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6068..6691)
                     /gene="iprA"
                     /locus_tag="ACHK0Z_RS21375"
                     /old_locus_tag="pgaptmp_004275"
     CDS             complement(6068..6691)
                     /gene="iprA"
                     /locus_tag="ACHK0Z_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGU4504196.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6779..>9413)
                     /locus_tag="ACHK0Z_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             complement(6779..>9413)
                     /locus_tag="ACHK0Z_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4504197.1"
                     /translation="QETRGGSESSDGHYVYQFSEYGTDRVIDDNWHDGDVFTLNIANS
                     TIDDDYEGLYFTDSYLDGDVTKYTNETFHTPAGEGEEYAGLFANGGVGLGLAVNLDVE
                     SNINISNNSRVAGISLTQGNTVNNTYTTESHTWDNNISVTDSTVTSGSVTTLEDSGFY
                     GNSAEPSDYSGNGEANDVALYFSDSAASNYSMKNNVYFSNSTLLGDVVFASTFNANFY
                     PQGHDSNADGVLDTNGGWADDSLNVDELNITLDNGSKWVGSATTSANVDDESTASTDW
                     YDVTGNSLYPGVVTEANAWGRTIDDQVFQSGVFNVTLNNGSEWNTVNASNIDTLAVNN
                     GSQVNVSESSLISDSIALTNGSSLNIGDEGYVGTDHLTVDSYSTVALSSDDNSSIYNG
                     YYTPSLLANTITVTNGGLLDINVDQFDDNPFQTDTLELTSGNIADHNGNVVSGVFDIH
                     SSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNV
                     VAATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNTNATFSAANKADLGAYTYQAEQR
                     GNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYF
                     GGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQD
                     SQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGY
                     DFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSE
                     DQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDAN
                     YLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2453 a         2350 c         2212 g         2398 t
ORIGIN      
        1 tattataaat aaaagttatg aaatatattc tcttcgttaa cttataatta agagaaaaaa
       61 actcaccgca tggccattta agccaggaat aaattagctt cccgttcatc atttttgtga
      121 tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag
      181 cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta
      241 tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt
      301 tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat
      361 gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg
      421 cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg
      481 cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac
      541 gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg
      601 ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt
      661 tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc
      721 gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc
      781 agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag
      841 ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat
      901 ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc
      961 gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc
     1021 accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta
     1081 gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct
     1141 tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac
     1201 gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg
     1261 aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg
     1321 gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc
     1381 cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaacccc
     1441 gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt
     1501 gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac
     1561 gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg
     1621 aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta
     1681 attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt
     1741 cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt
     1801 cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt
     1861 tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct
     1921 tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg
     1981 caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag
     2041 gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc
     2101 ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat
     2161 cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct ggcatgacat
     2221 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     2281 aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     2341 cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     2401 ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc
     2461 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac
     2521 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg
     2581 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt
     2641 atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     2701 cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt
     2761 tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcacaggat
     2821 taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg
     2881 cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg
     2941 gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac
     3001 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     3061 aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca
     3121 gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     3181 tcgcaggcat cttctgccgc acgacgctcg ccacatctat cgctttttcg ccctttttca
     3241 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     3301 aagatagagg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     3361 cctgaatttt gtcaccatcc agttcatgtt caaagctgcg cagacgtttg tagatagaca
     3421 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac
     3481 caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg
     3541 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt
     3601 tgaaatacat atagtgaaaa tagaggcgga aatagttttt ccgcacggcg ctaaacagct
     3661 cgcgtaccgt aggcggcgtc gcacgcgtgg catcgtcttc accataaacc agctctttac
     3721 ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca
     3781 ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg
     3841 gaatgtgccc gataatcggc agctccggca catgcgtgga gagcgttacc agcaccggca
     3901 ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg
     3961 aagcaaaacg catggtgtct tcctgcacac gctgtgcggc cccttcgata tgacgcagtt
     4021 gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac
     4081 tgacaaagaa gttgttcagc acactgatca ccacagcgat cagcgcaatc cccagaaaga
     4141 cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt
     4201 gaatcagatc atagaacggt gcataccagg cgttgacggc gaccccgact tccaccaaaa
     4261 accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg
     4321 ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa
     4381 aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc
     4441 cagtgatacg cgccacccac tctcccccac cggcttgcca gaagataacg gcaatcaatg
     4501 cccaaacaaa ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac ttaaacatat
     4561 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta
     4621 caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca
     4681 gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg
     4741 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     4801 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     4861 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     4921 tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc
     4981 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta
     5041 cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg
     5101 cagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg
     5161 tattgcttcc ctcaccaagc taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg
     5221 gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccaac
     5281 ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg
     5341 tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg
     5401 ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc
     5461 taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac
     5521 ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc
     5581 ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa
     5641 cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg
     5701 gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca
     5761 gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa
     5821 agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat
     5881 tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa
     5941 gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag
     6001 cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc
     6061 atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     6121 cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc
     6181 gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt
     6241 caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca
     6301 cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga
     6361 gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat
     6421 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca
     6481 taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa
     6541 cgccctctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct
     6601 tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa
     6661 attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca
     6721 aatccttgac gggcattatt tagattaaat taccagaatt tcttcggagt gaagaatatt
     6781 accaggtata tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga
     6841 ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac
     6901 caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat
     6961 cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctggtctt
     7021 cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag
     7081 actgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac
     7141 cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg
     7201 ctttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc
     7261 tcatggttgc agacaggtcg ttgttgaagt gagagtaact caagctacca tcaacaaaga
     7321 cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt ctggctgtct tgatccacct
     7381 gaccagaacg gtcattcatg tcacctttag cgaagcctgc agccgcaccg acgatccact
     7441 tagcgttgtt accgtcaatt ttggtatcaa caccgaccat gatgccgtta acatcctgat
     7501 catagttgat ggtgccgttg tcgccgttga agttaccacc gaagtagctt acccatgcgc
     7561 cgccgttatc agccaggcca tgacgagagt tggtcagacg agtaccaacg gtgtcttgtt
     7621 ccaggttcca gatattggtg ttcgcagacg ggatgctcag cgccatgtta gcgtagtcgg
     7681 tcagctccat ctgttgcaga acaacggtgt taccgcgctg ttcagcctga taggtgtatg
     7741 cacccaggtc agctttgtta gcagcagaga aggtcgcgtt ggtgttgacg tcgtttacgt
     7801 agataatttc tttatctttg taatcagcga tagcgccagc gcctgttgcg ttgtcgatac
     7861 gaactttgta gttaccggtt gcagcaacaa cgttgtcaac agagctgtta tccagttcgt
     7921 cgccgttgtt catgtcaccg ttaccgttga tagtcagatg accatcagag ttcatagcaa
     7981 caataccgta gccgtagtta gacttggaag tatcccaggt acggtcgttc accaaatcag
     8041 cgttcagaac ataatcgctg ctgtggatat cgaatacacc agacactacg ttgccgttat
     8101 ggtcagcgat gttgccgctg gtcagttcca aagtatcagt ctggaacgga ttatcatcga
     8161 actggtctac attaatatcc agtaaaccac cgttagtcac agttattgta tttgctaaca
     8221 gcgacggagt gtaataacca ttatagatac ttgaattgtc atcactagac agcgctacag
     8281 tactgtagct atcaactgtc agatgatcag ttccaacata accttcatct ccaatgttca
     8341 aagaagaacc attggtcaga gcgatgctat cagaaatcaa gctagattca gacacattaa
     8401 cctgagagcc attattcaca gctaaagtat caatgtttga agcgttaacg gtattccatt
     8461 cagaaccatt attcagagtg acgttaaaca caccgctctg gaatacttga tcatcaatcg
     8521 tacgacccca agcatttgct tctgttacaa cgcccggata caggctatta cctgtaacat
     8581 cataccagtc agtcgaagca gtagattcat catcaacatt tgctgatgta gtagctgagc
     8641 ctacccactt agaaccatta tccagagtga tattcagttc atcaacatta agtgagtcat
     8701 cagcccagcc accattggtg tccagtacgc catcggcatt actgtcatga ccttgaggat
     8761 agaagtttgc gttgaaagta cttgcgaaaa caacatcacc cagcagagta gaattgctga
     8821 aataaacatt gtttttcatg gagtaatttg aagccgcact atcactgaaa tacagagcta
     8881 catcattagc ttcgccgtta ccagagtaat cactaggttc cgcagagtta ccatagaaac
     8941 cactgtcttc cagagtagtt acagaacctg aagtcactgt ggaatcggta actgagatat
     9001 tgttatccca agtatgagat tcagtagtgt aagtattgtt tacggtatta ccctgagtca
     9061 ggctgatacc agcaacgcgg gagttgttag aaatattgat gttactttcg acatcaagat
     9121 tcactgccag cccaaggcca acaccaccgt ttgcaaacaa accggcgtat tcttcacctt
     9181 caccagctgg ggtatggaag gtttcgttag tatatttggt aacgtcaccg tccagatatg
     9241 aatcagtgaa gtataaacct tcataatcgt catcaattgt agaattagca atatttaaag
     9301 tgaatacatc accatcatgc caattatcat caattacacg atcagtaccg tattcagaga
     9361 actgatagac ataatgacca tcactgcttt ctgaaccgcc ccgggtttcc tgg
//
LOCUS       DAZFWP020000179         9386 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFWP020000179 DAZFWP020000000
VERSION     DAZFWP020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.179"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(79..957)
                     /gene="glxR"
                     /locus_tag="ACHK0Z_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             complement(79..957)
                     /gene="glxR"
                     /locus_tag="ACHK0Z_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4504198.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1057..1833)
                     /gene="hyi"
                     /locus_tag="ACHK0Z_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             complement(1057..1833)
                     /gene="hyi"
                     /locus_tag="ACHK0Z_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU4504199.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPKTTTEAGLRWMDPYR"
     gene            complement(1846..3627)
                     /gene="gcl"
                     /locus_tag="ACHK0Z_RS21395"
                     /old_locus_tag="pgaptmp_004279"
     CDS             complement(1846..3627)
                     /gene="gcl"
                     /locus_tag="ACHK0Z_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU4504200.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAKRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3717..4532)
                     /gene="allR"
                     /locus_tag="ACHK0Z_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             complement(3717..4532)
                     /gene="allR"
                     /locus_tag="ACHK0Z_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU4504201.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4610..5092)
                     /gene="allA"
                     /locus_tag="ACHK0Z_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             complement(4610..5092)
                     /gene="allA"
                     /locus_tag="ACHK0Z_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU4504202.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5322..6248
                     /gene="allS"
                     /locus_tag="ACHK0Z_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             5322..6248
                     /gene="allS"
                     /locus_tag="ACHK0Z_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU4504203.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPFGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6317..7411
                     /gene="mnmH"
                     /locus_tag="ACHK0Z_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             6317..7411
                     /gene="mnmH"
                     /locus_tag="ACHK0Z_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU4504204.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7527..7892
                     /gene="ylbG"
                     /locus_tag="ACHK0Z_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /pseudo
     CDS             7527..7892
                     /gene="ylbG"
                     /locus_tag="ACHK0Z_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8413..8904)
                     /locus_tag="ACHK0Z_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     CDS             complement(8413..8904)
                     /locus_tag="ACHK0Z_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /inference="COORDINATES: protein motif:HMM:NF011298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504205.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFITNIGAGLPDA
                     CAE"
     gene            complement(8885..>9386)
                     /locus_tag="ACHK0Z_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     CDS             complement(8885..>9386)
                     /locus_tag="ACHK0Z_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4504206.1"
                     /translation="GWRGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAW
                     DILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNL
                     FGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTIKALQ
                     DAGYLK"
BASE COUNT         2431 a         2423 c         2128 g         2404 t
ORIGIN      
        1 caacatcttt ggctgctttc tttttttaat attgctggcc gattcatttt ctgatatcgg
       61 ccatttttat tgcgggtatc aggccagttt atggttagcc attaattcca gcgcctgcac
      121 taacgcagag tgatccaact ggctgccacc gtttgccgcg caggtattaa ataactcctg
      181 gcaggtcgca gtgtttggca ggttcagcgc tagcgctttc gcactttgca gtgccaggtt
      241 gagatctttc tggtgcagcg caattttgaa acctggatta aaagtgcgtt taatcatgcg
      301 ctcaccgtga acttccagaa tacgtgagga agcaaagccg cccatcagcg cctggcgcac
      361 acgtaccggg tccgcaccgg cttttgaagc aaatagcagg gcttcagaaa ccgcttcaat
      421 attgagcgcc acaataatct gatttgccac tttgcaggtt tgaccatcgc cgttaccgcc
      481 cacgagggtg atatttttac cgagcagttc aaacagcggt ttaacacgtt caaataccgc
      541 ttcatcaccg ccaaccataa tcgacaacgt cccttcacgc gcaccgattt caccgccgga
      601 gactggcgca tcgagataat cgccgcccag ttcattcacc tgacgagcga aacgcttagt
      661 ttcaatcggg gaaatggagc tcatatcaac aatggttttg cccttcagcg aggctttggt
      721 acaaccattt tcaccgaaca gaacttcttc aacctgtggt gtatctggca ccataataaa
      781 aatgatgtcc gatgcttccg ttacctggcg cgcagtttca acactgacgg cacccagtga
      841 cagtaattca tcagcaaccg gaccaatggt tgtgacatgc agttgatgac cggcacgcgc
      901 cagattaatg gccatcggtg tacccataat gcctaagcca ataaatccca gtttcataat
      961 taacctcttt taaatttcgc ttttcctgaa ttcagacaac acgatcccct gccaacttag
     1021 cggcctaaaa aagcattgcc tgaacagcgt tacgttttaa cggtacggat ccatccagcg
     1081 taaaccggct tccgtggtgg ttttgggtgt atattcacac ccaacccaac cgttgtagtc
     1141 agaattttcg attaccttaa agagataatc ataattaatt tctccggtcc ccggttcgcc
     1201 gcgatgcgga ttatcagcaa tttgcaggtg accaatttta tcggcccact gagtcatggt
     1261 gttggttaat tcaccttcca tccgctgcat gtgataaatg tcatactgaa ttttcaaatt
     1321 gcagcaacca acatcatcaa tcaatttcag cgcctgccga gttccggtga gatggaaacc
     1381 aggaatatca aaatggttaa taggttcaat cagtaataaa atatcttctt tcatcagcat
     1441 attcgcggca taacgcaggt tttctacaag cgttgcgtga atctgttcac tgctgaaacc
     1501 agccggcgtt ttaccgacca gacagttaat ttttttatta cccagcgcac gggcataacg
     1561 aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac aggcaatacc
     1621 gcgctccccc gccgcccagt cacctgccgg taaattgtgc agcgtatgtt cgagtttatt
     1681 actcgccagc acctgtttta attcttcaat gtcgtagtca taaggaaaca taaattcaac
     1741 gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat attcaccaaa
     1801 taacatcgat aaattagcag agaaacgtaa cattatttat ctcccttatt catagtgcat
     1861 gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa attccatgac
     1921 gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa cttccacgac
     1981 taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg gcgcaatatc
     2041 ttccggtttg aagacgcgaa tagctttaca acctaaacct tccgctactt ttacgtggtc
     2101 aacaccgtag ccattcactt cactggagtt gatattctcg aaagcgagtt gcacgcagta
     2161 gtccatatca aacgcgcgct gcgactggcg aatcagcccc agataagcgt tattgaccag
     2221 tacatggatg tacggaatgt tgaactgtgc gccaacggcc aattcttcaa tcaggaactg
     2281 gaagtcgaag tcaccggaaa tcgccaccac tttgcgctct ggatcagcgg cacaaacccc
     2341 cagcgccgcc ggaatggtcc agcccagcgg gcctgcctgc ccacagttga tccagtggcg
     2401 gtctttaaag acatgcagca tttgcgcagc ggcaatttgc gacagaccaa tggtggtgac
     2461 atagcaaaca tcacgtccaa aagctttgtt catctcttca tacacacgct gcggtttaac
     2521 cggaacgttg tcgaagtgtg ttttacgcaa caaagtacgt ttgcgctgct ggcactcagc
     2581 aacccactct ttacggcatg gcagacgccc tgctttttgc atttcctgcg ccacttcaac
     2641 cagcagtgtc agcgccgctt tagcatcaga gacaatcccc agatccggac acagcacgcg
     2701 gccaatttgc gtcggctcaa tatcgatatg aacgattttg cgcccttcgg tgtatttctc
     2761 taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca tgtcggacgc
     2821 cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga cccaccatcc cggccatcag
     2881 ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg gaacgctggt
     2941 cagttcagca aactgttgta acagtgcagc tgcgtcagca ttaattaccc cgcccccggc
     3001 aacaatcacc ggacgtttgg cctggattaa catttctaca gctttttcga tctgcatacg
     3061 gctggcagca ggtttgtaga ccggcagcgg ttcgtacata tcaggatcaa actcgatttc
     3121 cgcaacctga acgtcgaacg gtaaatccac cagtaccggt cccggacgac cagaacgcat
     3181 caggtgaaat gcctgttgca gcacacgagg caccagcgcc gcttcacgta ctgtaaccgc
     3241 cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat cttctttatg
     3301 cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat cagcagaagc
     3361 ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc
     3421 gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaagcac cttccacatg
     3481 acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga acggattgat
     3541 tgcagctccc ggaacaccga aggcggtggt gataccttct ttctccagca catacattgc
     3601 cgcgtcaacg gctctcattt ttgccatttt attcctacct ctatttattg gaaaattttt
     3661 ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga cagacattat
     3721 ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct gaccagctca
     3781 ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat ggagatagcg
     3841 gcaacaacgc taccgacatc atcgtaaatt gctgaagcta tgcaattcag acctacaaca
     3901 tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc cttcagcaag
     3961 gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg cagaatgatg
     4021 ctcatcaact cctcttccgc cagcggataa agcaacgcct tgcccgcacc ggaagcatgc
     4081 agcggcagac gactgcccag tggcgcacac atcctgacca ttgatttaca ctctaactga
     4141 ccaattaata ctgcttcatt gccgttacgg atcgcgacat tgaccgtttc gccggaaagt
     4201 aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt atggatgtac
     4261 gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaattg actgtcctga
     4321 tagacaaaat ccgctgcttg taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa
     4381 tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc cagatattgc
     4441 agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg ctccgcctgt
     4501 cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac ctacaagtga
     4561 gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac
     4621 agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg cgatcgatgg
     4681 tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg ttacgatggt
     4741 aattcactcc ctgttcgccg ttggtgataa acgcccgcag cgttgacagg tctggtttgt
     4801 cgtcgcctaa cgccacgacc accacaaaca cttcaccttt catcgggata aaggcctgag
     4861 tgcccagcgg atgacgttcg agttcgtaaa tggtcagcgg cagattcgcc ggttgcgcgc
     4921 ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc aaatcgtggt
     4981 aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgagtt tcgattacgt
     5041 cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc atctttttgt
     5101 ccttgtaaca taattccgtt caaccatcgc cgaccggtca gcttggtttc aataacaggc
     5161 aatctaaaac cttaagagaa caacatcaat acaaaagttg gaatattttt ccaaaactag
     5221 atttagtcca caaattgaac agggttatca tgtaaaaata ctgataaatc agaggataag
     5281 aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat ccagaaacct
     5341 tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca gaacgattat
     5401 gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat accggagtag
     5461 cgctgttttt ccgcacgact cgcagcgtga cgttgacagc ggctggcgag catctacttt
     5521 cccaggccag agactggctg agctggctgg aaagtatgcc aagcgagctg caacaggtga
     5581 atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctac cacccccagg
     5641 ccgtcgccca gttgctggcg tggctgaatg aacgttaccc ctttacccag tttcacatct
     5701 cccgacaaat ctatatgggc gtctgggatt cgctattata cgaaggtttt tcactggcta
     5761 tcggcgtcac gggaaccgag gcgctggcaa atacctttag tcttgatccc ttcggatcgg
     5821 tgcaatggcg ctttgtcatg gcggcggatc atccgctggc gaacgttgaa gagccgctaa
     5881 cagaagcgca gttgcggcgc tttccggcgg tcaatattga agacagcgcc cgcaccttaa
     5941 ccaaacgcgt cgcctggcga ttgcctgggc aaaaagagat tattgttcct gatatggaaa
     6001 cgaaaatcgc cgcccatctt gcgggcgttg gcattggttt tttgccaaaa tcgctttgcc
     6061 agtcaatgat cgataatcaa caactagtta gccgggtaat cccaacgatg cgccctcctt
     6121 cgccattgag tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa gatattgtga
     6181 ccttgtttac ccagcgcagg ccggaaatca gcggattttt agaaattttc ggcaacccac
     6241 gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca agctgatttg
     6301 aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg ccctgctgat
     6361 tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag gcgcaatgcc
     6421 cgccgctatc aatctgccgt taatgaataa cgatgaacgc gccgccgttg gcacctgcta
     6481 taaacagcaa ggctcagacg cagcgctggc gctggggcat aaactggtgg cgggtgaaat
     6541 tcgtcagcag cgcatggacg cctggcgggc agcgtgcctg caaaatccgc aaggtattct
     6601 ctgctgcgcc cgtggcggtc agcgctcaca tattgtgcaa agctggttgt atgcagcggg
     6661 gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg cgattcaggc
     6721 gactattgaa ctggcacaaa aaccgatagt gctgattggc ggttgtactg gcagcggtaa
     6781 aacgctgtta gtgcagcaac agccgaacgg tgttgatctg gaagggctgg cgcgccatcg
     6841 cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt ttgaaaacct
     6901 gctggctgcc gaaatgctga aaaccgacgc ccgtcaggat ttgcgcctgt gggtgctgga
     6961 agacgaaagc cggatgatcg gttcgaatca cctgccggaa tgcctgcgcg agcgaatgac
     7021 tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgagc gcctgaacga
     7081 agagtatttc ttgcgtatgc atcatgattt tacccacgct tatggcgacg aacagggctg
     7141 gcaggaatat tgcgaatacc tgcatcacgg actttcggcg attaagcgtc ggctggggtt
     7201 acagcgctat aacgaactcg ctgcccagct ggatacggcg ctgacaacgc aactcaccac
     7261 cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttaaagaat attacgaccc
     7321 aatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg gtgagtgggc
     7381 ggaagtggcg gaatgggtta aggcgcggta atgagggacg tatttgtatt agtcgcgact
     7441 tgatctgaca catggccttg aaaggttgag agttaccggt tttgatatga gtgtcgaatc
     7501 cttatccaaa acaagaggta actctcatgc ttcatactgc caatcccgtc atcaaacaca
     7561 aagccggttt gctcaatctg gctgaagaac tcagcaacgt atcaaaagcc tgtaaaatca
     7621 tgggcgtttc gcgtgatacg ttttatcgtt accgcgaact ggtcgctgaa ggcggtgtgg
     7681 atgcgcagat taatcgtagt cgccgcgctc ctaaccttaa gaaccgtacc gatgaggcaa
     7741 ctgaacaggc tgttgttgat tacgccgtcg ctttcccgac acatggtcag caccgggcca
     7801 gcaacgagct gcgtaaacag ggggttttta tctccgacag tggtgtccgt tctgtctggt
     7861 tactccataa ccttgagaat ctcaaaagac gatactgaac aaattttatc aggtgacgtt
     7921 ccgtaaaaag ttgtatggag ttctcgatgc agtcggcgcg actatcagta cccccgctga
     7981 accctggact atcgggccgc cgtactgggg catgtcaccc tggcgtgccg ctggttttcc
     8041 gctaatggtt tatttcctct ttctttcccg gcaagaattt attttaatcc ggaaggatta
     8101 tttattttca gatagggact cacattaaac acgtaacatt ttaattaatg catggctgaa
     8161 tatgtattac acaatgtcag atatcgtaaa agaccagtgc aattctatgt aaactctctg
     8221 actgttcatt ttatttgttg tttcagggtc ggaactcacc ctcaataaac aactgaatac
     8281 gagtagatat tcatcgtctg agctatatgg ctttacacaa tagccgacat gtcttttctc
     8341 agtcaggaat tttacttttc ctcactacga agttgacgac ttcgatatgg gatagactct
     8401 taattcaagc aattactctg cacaagcatc aggcaatcca gctccaatat ttgttataaa
     8461 ataatgagta acattaattt caccaacaaa aaaaaggttt acaccatccc cttgccctgg
     8521 tatcttacca tatttacacc acactttttt tgtatctgga tcattctttg atacgatttt
     8581 cgccacttgt tccctggccc tcactgtcaa tgttttctta aacctacccc catcatcatt
     8641 tgttagggaa aaataagaaa aaaaataatc atcttctaca tttgtgtata ttgatatatc
     8701 caccgctacg tgcggaaaaa aatctgggag ccatgtaaaa actccatcat tttctggtat
     8761 tgccatatag tttatgtaat gtttacttac aagagattca tctttgtata aataaagata
     8821 agtaattacg cataaaacaa caatgattat aatagcaaaa ataaatatta tcatctttga
     8881 tagattactt gagatagcca gcatcttgta aagcctttat cgttttttta tgctctggat
     8941 taatataatc actacatcta tctgagcaat ctgttgttga tggacatgtc aacccatggt
     9001 catttacagc caagtctttt ttattatctt caatcatttc agaatgggat agctttactt
     9061 ttctgccgta cataccgaac agatttaacc cgtaatctct aatctctttt tcataaccca
     9121 gccctttcgc cgatcggcta acaccagcgt catttaattt agacactcga caaaatgcca
     9181 tacaatggaa aaattgatca gtcccaccta aatttaatct cttcatatct tcataagtat
     9241 cagataatat atcccatgct ctttgatggt ttagttgatc ttttcttctt attagcgcta
     9301 catctgcggg acttaaccct aatggatcaa taccattcac cggattcagc gggtacgcat
     9361 acagactcca tccccccctc cagccc
//
LOCUS       DAZFWP020000180         9194 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFWP020000180 DAZFWP020000000
VERSION     DAZFWP020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.180"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            25..909
                     /gene="xapR"
                     /locus_tag="ACHK0Z_RS21435"
                     /old_locus_tag="pgaptmp_004287"
     CDS             25..909
                     /gene="xapR"
                     /locus_tag="ACHK0Z_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HGU4504207.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLRIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(1018..1093)
                     /locus_tag="ACHK0Z_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     tRNA            complement(1018..1093)
                     /locus_tag="ACHK0Z_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1058..1060),aa:Lys,seq:ttt)
     gene            complement(1098..1173)
                     /locus_tag="ACHK0Z_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     tRNA            complement(1098..1173)
                     /locus_tag="ACHK0Z_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Val,seq:tac)
     gene            complement(1220..1295)
                     /locus_tag="ACHK0Z_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     tRNA            complement(1220..1295)
                     /locus_tag="ACHK0Z_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1260..1262),aa:Val,seq:tac)
     gene            complement(1340..1415)
                     /locus_tag="ACHK0Z_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     tRNA            complement(1340..1415)
                     /locus_tag="ACHK0Z_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1380..1382),aa:Val,seq:tac)
     gene            1674..3089
                     /gene="gltX"
                     /locus_tag="ACHK0Z_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             1674..3089
                     /gene="gltX"
                     /locus_tag="ACHK0Z_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU4504208.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(3141..3533)
                     /locus_tag="ACHK0Z_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             complement(3141..3533)
                     /locus_tag="ACHK0Z_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4504209.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3535..3894)
                     /locus_tag="ACHK0Z_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             complement(3535..3894)
                     /locus_tag="ACHK0Z_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4504210.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4115..4190
                     /locus_tag="ACHK0Z_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     tRNA            4115..4190
                     /locus_tag="ACHK0Z_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4148..4150,aa:Ala,seq:ggc)
     gene            4230..4305
                     /locus_tag="ACHK0Z_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     tRNA            4230..4305
                     /locus_tag="ACHK0Z_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4263..4265,aa:Ala,seq:ggc)
     gene            4513..6702
                     /locus_tag="ACHK0Z_RS21485"
                     /old_locus_tag="pgaptmp_004297"
     CDS             4513..6702
                     /locus_tag="ACHK0Z_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4504211.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(6752..7954)
                     /gene="nupC"
                     /locus_tag="ACHK0Z_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             complement(6752..7954)
                     /gene="nupC"
                     /locus_tag="ACHK0Z_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU4504212.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            8290..>9194
                     /locus_tag="ACHK0Z_RS21495"
                     /old_locus_tag="pgaptmp_004299"
     CDS             8290..>9194
                     /locus_tag="ACHK0Z_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU4504213.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSS"
BASE COUNT         2232 a         2164 c         2375 g         2423 t
ORIGIN      
        1 atttattata tggagcactt aattatggaa cgcgtataca gaacagatct taagttgctc
       61 cgttattttc ttgccgtagc ggaagagttg cattttggcc gcgcagcagc gcgtttaaat
      121 atgtctcagc ctccgctcag gattcatatt aaagagctgg aaaatcaact cggcacgcag
      181 ctttttattc gccattcgcg cagcgtcgta ctgacacacg cgggcaaaat cttgatggaa
      241 gaatcgcgtc gattgctggt gaatgctaat aatgtattgg ctcgggttga acaaataggt
      301 cggggagaag cagggcggat tgaactcggc gtagtgggaa cggctatgtg gggacggatg
      361 cgcccggtta tgcggcgatt cctcagggaa aatcctaacg ttgaagttct ttttcgcgaa
      421 aagatgcccg cgatgcaaat ggccctgctg gaacgacgtg aactcgatgc cgggatctgg
      481 cgaatggcga cagaaccacc gacaggtttt accagcttac ggttgcatga atcggcgttt
      541 ctggtggcga tgcctgaaga gcatcatctc tcatcatttt ccaccgttcc gctggaagcg
      601 ctacgtgacg agtattttgt tacaatgccg cccgtgtaca ctgactggga ttttttgcag
      661 cgagtttgcc agcaggtggg attttcaccg gttgttattc gcgaagttaa tgaaccgcaa
      721 acggtactcg ccatggtcag tatgggtatt ggtatcacat tgatagcgga cagctacgca
      781 caaatgaact ggccaggtgt cattttccgt ccgctcaaac agcgcatccc tgcagattta
      841 tatattgttt atgaaacaca gcaggtgacg cccgcgatgg ttaagctgtt ggctgcattg
      901 acacagtagg tgaggagaca ggaattgccg gaaatagtcg tctgctaagt gaagcgcatc
      961 aggcaatttc gcaaatcgca gatagcaaaa aagcgccttt agggcgcttt tttacattgg
     1021 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
     1081 gagctaacga cccgaagtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta
     1141 agggaggtgc tctcccagct gagctaatca cccacttcgg tacttcacat ttttacaaga
     1201 aatttagctg gcgagaaagt ggtgggtgat gacgggatcg aaccgccgac cccctccttg
     1261 taagggaggt gctctcccag ctgagctaat cacccacttc tcaatttctt gctacacggc
     1321 ggagactaca taaagtagtt ggtgggtgat gacgggatcg aaccgccgac cccctccttg
     1381 taagggaggt gctctcccag ctgagctaat cacccccgct gtgtggagtc gcattatagg
     1441 gagagttcaa aatgagtcaa cgcattttct aaagaaattg ttcgttcgtc gtaaatttaa
     1501 gcaagatgat cgcaaaacag accgtgttgc gcaatttgtc aacgaaaaca ataatgcgta
     1561 aggtagaaac ccgaactaca ttgaggaatc aggcgggagt gatagaatat cgcccactca
     1621 atttttccag gatttgccgg ttgtcggcat ctttctaaac gtaaggccat ttcatgaaaa
     1681 tcaaaactcg cttcgcgcca agcccaacag gctatctgca cgttggcggc gcgcgtactg
     1741 ctctttactc ctggcttttt gcacgtaacc acggcggtga gttcgtgctg cgtattgaag
     1801 acaccgatct tgagcgttcc acgccggaag ctatcgaagc cattatggat ggcatgaact
     1861 ggctgagcct ggagtgggat gaaggtccgt actaccagac caaacgtttt gatcgctaca
     1921 atgcggtgat cgatcagatg ctggaagagg gcactgctta taaatgctat tgctctaaag
     1981 agcgcctgga agcgctgcgc gaagagcaaa tggcgaaagg tgagaagccg cgttatgacg
     2041 gtcgctgccg ccacagccat gagcatcatg ctgatgatga accgtgtgtc gtgcgttttg
     2101 ctaatccgca ggaaggttct gttgtttttg acgatcagat ccgtggtccg atcgagttca
     2161 gcaaccagga gctggatgat ctgattatcc gccgtaccga tggttcccca acctataact
     2221 tctgtgtggt tgtcgatgac tgggatatgg aaatcaccca cgttatccgt ggcgaagacc
     2281 atatcaacaa cacgccgcgc cagatcaaca ttcttaaggc cctgaaagcg ccggtgccgg
     2341 tttacgcgca cgtttctatg atcaatggcg atgacggtaa aaaactgtcc aaacgtcacg
     2401 gggcagtcag cgtaatgcag tatcgtgatg acggttattt gccagaagca ctgctgaact
     2461 atctggtgcg tctgggctgg tcccacggcg atcaggaaat cttcactcgt gaagagatga
     2521 tcaaatactt cactttgaat gccgtcagca aatctgccag tgcgttcaac accgacaagc
     2581 tgctgtggct gaaccatcac tacattaacg cgctgccgcc ggagtatgtt gctactcact
     2641 tacagtggca cattgagcag gaaaatatcg atacccgtaa cggcccgcag ctggctgatc
     2701 tggtgaaact gctgggcgaa cgctgcaaga cgctgaaaga gatggcacag agctgccgtt
     2761 atttctacga agattttgct gagttcgatg ccgatgcggc gaaaaaacat ctgcgtccgg
     2821 tagcgcgtca gccgctggaa gtggttcgtg acaaactggc cgcgattact gactggaccg
     2881 ctgaaaacgt tcatcacgct attcaggcga cggcggatga gctggaagtg ggtatgggta
     2941 aagttggtat gccgctgcgt gtcgccgtaa ccggtgcggg gcagtctcca gcactggatg
     3001 ttaccgttca cgcaattggt aagacccgca gtatcgagcg tatcaacaaa gcgctggatt
     3061 ttattgctga acgcgaaaat cagcagtaat tagcgtgtaa aaagataaac ggcaggagat
     3121 aatatcctgc cgttttttat ttatgctgat tcgtcaatat cgagacgggc gaggaaattg
     3181 cgaattcctt cacgtgacaa aaattgcgcg actttttcct gctcaatgtc tgtcatatta
     3241 tctatcgcgt tgatgatttg tcgatacgcc ggggcgcgtg ctccaggagc gtattcagca
     3301 attctctctt cagcttccat caacattggt gtgttatgga aggctggcgt gttctgaata
     3361 aactcgcaaa cttgcgtatc gaccagaata agtctggcac gaccgccttt cacgccggga
     3421 aatttttccg ttttccagcc tttttctctg atccaacggt taacagtttg tttagccaca
     3481 ccgaggcatt cagccagttc ttcggtggtc attttgttgc gtaatctttt catattattt
     3541 gctatcgcga atccctaagc gttgtaacaa tccgataatc ccttcccgca gaagcaagga
     3601 tgtaaactgt ttttgttcaa ctggcgtcat ctctttcgcc agtgtcacca gcaatacttc
     3661 aagcggcgca tcaccagaaa gggcaggcgc ttctccctga ccttctggac gttcagcatt
     3721 gcgaatatat tcacgaacct gttcattgac gtgaaccagt cgggctttgc caccctggac
     3781 gccaggtttt ggtgacgttg tccagccttc cttgcgtacc catttattaa tggtctggcg
     3841 gctatagccg gtcagtctgg caagttcatc tggtgtcatc ctctccttga acataacaat
     3901 tccctgaatg tatgtgggtc tattagtagt tcattttata acaccacttt acaggaaacc
     3961 gtgacatgaa taactactga atgcgagcgg ttttccaaaa ttgttcattt gcctgatttt
     4021 tcagcgattg aaattacacg ttgcaatttg ccgttgacac attcgggcgg aatttatatg
     4081 atgccgcccg tcaactcgac aagctttacg tgacggggct atagctcagc tgggagagcg
     4141 cttgcatggc atgcaagagg tcagcggttc gatcccgctt agctccacca aattttgcac
     4201 ccagcaaatt tggtacgtaa acgcatcgtg gggctatagc tcagctggga gagcgcttgc
     4261 atggcatgca agaggtcagc ggttcgatcc cgcttagctc caccaaattt ccaaccctcg
     4321 ctgcaaagcg ggggtttttt gtctctgctt tttgccgctt ttgtcataca gtctacgtcc
     4381 gggttagcgc cgcctggtga aagcatcatt ggatgaaaaa tcggcaacag gctggcccct
     4441 gtttgcttcg cgatgcgaat aaacttatta tttgtgtgcc tgaaaacccc gatcagtgag
     4501 agtagtgtac tcatgtttgt ggagcataac ctgataaaaa atatcaagat attcacacta
     4561 gcgtttacgc tcaccgtggt acttattcag ctatcccgtt ttatttcgcc acttgccatt
     4621 atccattcca gttatatctt tctggcgtgg atgccactgt gcgtaatgct gtcaatcttg
     4681 tttatctttg gctggcgcgg tgtcgttccc gttttatgcg ggatgttttg caccaatctg
     4741 tggaactttc atctctcttt tttacagact gcggtcatgc tcggtagcca gacgtttgtc
     4801 gtgttgtgtg cctgcgcaat attacgctgg cagctgggga cgcgttggcg ttatggattg
     4861 accagccgat atgtctggca acgtctgttc tggcttggtt tggtgacgcc gatcggcatc
     4921 aaatgcagca tgtatcttgt ggggagcttc tttgatttcc cgctaaagat atccaccttt
     4981 ttcggcgatg cggatgccat tttcacggtc gttgatttgc taagcctttt caccgctgtg
     5041 ctgatttaca acatgctttt ctactatctc actcgcatga ttgtaagtcc ccactttgcg
     5101 cagatattgt ggcgcaggga tatcgcgccg tcgttgggca aagagaaacg cgcatttacc
     5161 ttaagctggc tggcagcgtt aagcgtgctt ctacttctgt tgtgcacacc ttatgaaaac
     5221 gactttattg ccggttacct ggtacccgtt ttcttcatca tctttaccct cggggtcggt
     5281 aagcttcgct atccgttttt aaatctcacc tgggctgttt caacgctttg ccttctgaat
     5341 tacaaccaga actttttgca aggggtggaa accgaatatt cgctggcatt tattcttgcg
     5401 gtgctgattt cctttagcgt ttgcctgctc tatatggtgc gcatttatca tcgcagtgaa
     5461 tggcttaatc gccgctggca tttgcaggcg ctgacagatc cgttaacgct cctacccaac
     5521 tttcgtgcgt tggaacaagc gccggaacaa gaggcgggca agagtttttg ctgcctgcgc
     5581 attgataatc ttgagtttat gagtcgtcat tacggcttaa tgatgcgcgt tcactgtatc
     5641 cgctcaattt gccgtacgct gctgccgttg atgcaggaaa acgaaaagtt gtatcaattg
     5701 ccgggtagtg aactgctgtt agtgctgagc gggccggaaa cggaagggcg actccagcat
     5761 atgattaaca tcctgaatag tcggcaaatt cactggaaca ataccgggct ggatatgggc
     5821 tatggtgctg cctgggggcg ttttgatgga aatcaggaaa ccctgcaacc cttgttgggg
     5881 cagttaagct ggctggcgga gcaatcctgc gcacatcatc atgtgctggc gctggatagc
     5941 agagaggaga tggtttccgg gcagaccact aaacaggtgc tattgctgaa taccattcgc
     6001 acggcgttag atcagggtga tttgctgctc tacgcccagc caattcgcaa caaagagggt
     6061 gaaggttatg atgagatcct cgcgcgactg aaatatgacg gcggcattat gaccccggat
     6121 aagtttctgc cccttattgc tcagtttaac cttagcgcgc gttttgattt gcaagtgctg
     6181 gaatccttgt tgaagtggct ggcaacacac ccttgcgaca aaaaaggacc gcgcttttca
     6241 gtcaatttaa tgccgctcac gctgctgcaa aagaatattg ccgggcggat tattcgtctg
     6301 tttaagcgtt atcacatctc cccgcaggcg gtcattcttg agatcaccga ggagcaggcg
     6361 ttttctaacg cagaaagcag catgtacaac atcgagcagc tgcataagtt tggtttccgg
     6421 attgcgattg atgactttgg caccggatat gccaactacg aacggttaaa gcgtttgcag
     6481 gctgatatca tcaaaattga tggcgtcttt gtgaaagata ttgtcacgaa cacgctggat
     6541 gcgatgattg tgagatcaat taccgatctg gcgaaagcga agtcattgag tgtggtcgcg
     6601 gagtttgtcg agacgcaaca gcagcaggcg ctattgcata agctcggggt gcaatatctg
     6661 caagggtatt tgattggtcg cccgcagcca ttagctgatt aacaggcgta aaaaaaccgg
     6721 ggaattatcc ccggcttttt tatgtatggt cttacagcac cagtgctgcg attgacgcag
     6781 acagcacact caccagggta gagccgtaaa ccagcttcag accgaagcga gaaaccacgt
     6841 taccttgctc ttcattcagg cctttaactg cacctgcgat aatcccgatt gaagagaagt
     6901 tagcgaagga aaccaggaac acagagatga tgccttcagc acgcggagag agcgtggaag
     6961 caattttctg cagatccatc atcgcaacga actcgttgga aaccagtttg gtcgccatga
     7021 tactgcccac ttgcagtgct tcactggaag gaacacccat cacccatgca atcggataga
     7081 agatgtagcc caggatgccc tggaaggaga tgctgtagcc aaaccagcca gtaacggtgg
     7141 caaacagtgc gttcagcgcg gcgatcaggg cgataaagcc gatcagcatc gcggcaacga
     7201 taatggcaac tttgaaacct gccagaatgt attcacccag catttcgaag aagctctgac
     7261 cttcgtgcag gttggacatc tggatgtttt cttcactggc atcaacacgg taaggattga
     7321 tcagcgacag cacgataaag gtgctgaaca tgttcagtac cagcgcagca acgacgtatt
     7381 tcggttccag catggtcatg tatgcaccaa cgatggacat cgacacggtg gacattgccg
     7441 tggcagccat ggtgtacata cgattacggg agattttgcc gaggatatct ttataggcaa
     7501 taaagttttc agactgaccc agaatcaggg agctgacggc gttaaaggat tccagtttgc
     7561 ccataccgtt gactttggag agcaggaaac caattgcgcg gataataacc ggcaacacgc
     7621 gaatgtgctg gagaataccg atcagtgcag agataaagac gattgggcac agcactttca
     7681 ggaagaagaa tgccaggcct tgatcattca tgctaccaaa gacgaagtta gtcccttcgt
     7741 tggcaaatcc gagcagtttt tcgaacattt cggagaagcc tttcacgaag cctaaaccaa
     7801 cgtcggagtt caggaagaac cacgccagta acacttcgat aacaagcagt tgaataacat
     7861 aacggatacg aatttttttg cggtcgctgc ttaccagcag tgcgagaatc gcaacaacgg
     7921 caagtgccag tacaaaatga aggacgcggt ccatatttgc tccaaatatg aggcaggtta
     7981 aatttccgtg cacattctat gtaacatggt taaggaaaac gagatctgac acacactata
     8041 atgacgtcct gtgacaagct tcaaaaatag tgatctgtca tacatttctg ctatgttgtg
     8101 tatggaagct gaaagttatg taaatgtgct aacattaaac ataaccataa cattcaaatc
     8161 atcaatcttt tcgtagtcat aatcctggtc tatcaaagaa atcaccacaa cccattcaaa
     8221 tgaatttgat aatcattctc gtttggcata gcatgaaaca tagcaaaggc tatgttttag
     8281 aggcacaaga tgacgaacta tcgcgttgag agtagcagcg gacgggcggc gcgcaagatg
     8341 aggctcgcat taatgggacc tgcgttcatt gcggcgattg gttatatcga tcccggtaac
     8401 tttgcgacca atattcaggc gggtgctagc ttcggctatc agctactgtg ggttgtcgtt
     8461 tgggccaacc tgatggcgat gctgattcag atcctctctg ccaaactagg gattgccacc
     8521 ggtaaaaatc tggcggagca gattcgtgat cactatccgc gtcccgtagt gtggttctat
     8581 tgggttcagg cagaaattat tgcgatggca accgacctgg cggaatttat tggtgcggcg
     8641 atcggtttta aactcattct tggtgtttcg ttgttgcagg gcgcggtgct gacggggatc
     8701 gcgactttcc tgattttaat gctgcaacgt cgcgggcaaa aaccgctgga gaaagtgatt
     8761 ggcgggttac tgttgtttgt tgccgcggct tacattgtcg agttgatttt ctcccagcct
     8821 aacctggcgc agttgggtaa aggaatggtg atcccgagtt tacctacttc ggaggcggtc
     8881 ttcctggcag caggcgtgtt aggggcgacg attatgccgc atgtgattta tttgcactct
     8941 tcgctcactc agcatttaca tggcggttcg cgtcaacaac gttattccgc caccaaatgg
     9001 gatgtggcta tcgccatgac gattgccggt tttgtcaatc tggcgatgat ggctacagct
     9061 gcggcggcgt tccacttttc tggtcatact ggtgttgccg atcttgatga ggcttatctg
     9121 acgctgcaac cgctgttaag ccatgctgcg gcaacggtct ttggattaag tctggttgct
     9181 gccggactgt cctc
//
LOCUS       DAZFWP020000181         9182 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFWP020000181 DAZFWP020000000
VERSION     DAZFWP020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.181"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            156..803
                     /gene="fucA"
                     /locus_tag="ACHK0Z_RS21500"
                     /old_locus_tag="pgaptmp_004300"
     CDS             156..803
                     /gene="fucA"
                     /locus_tag="ACHK0Z_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU4504214.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            831..1979
                     /gene="fucO"
                     /locus_tag="ACHK0Z_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     CDS             831..1979
                     /gene="fucO"
                     /locus_tag="ACHK0Z_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4504215.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2034..2789)
                     /gene="xni"
                     /locus_tag="ACHK0Z_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             complement(2034..2789)
                     /gene="xni"
                     /locus_tag="ACHK0Z_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU4504216.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2901..4268)
                     /gene="sdaB"
                     /locus_tag="ACHK0Z_RS21515"
                     /old_locus_tag="pgaptmp_004303"
     CDS             complement(2901..4268)
                     /gene="sdaB"
                     /locus_tag="ACHK0Z_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU4504217.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4326..5615)
                     /gene="sdaC"
                     /locus_tag="ACHK0Z_RS21520"
                     /old_locus_tag="pgaptmp_004304"
     CDS             complement(4326..5615)
                     /gene="sdaC"
                     /locus_tag="ACHK0Z_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU4504218.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(6172..7536)
                     /gene="ppnN"
                     /locus_tag="ACHK0Z_RS21525"
                     /old_locus_tag="pgaptmp_004305"
     CDS             complement(6172..7536)
                     /gene="ppnN"
                     /locus_tag="ACHK0Z_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU4504219.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(7648..8496)
                     /gene="queF"
                     /locus_tag="ACHK0Z_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             complement(7648..8496)
                     /gene="queF"
                     /locus_tag="ACHK0Z_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU4504220.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8564..9109
                     /gene="syd"
                     /locus_tag="ACHK0Z_RS21535"
                     /old_locus_tag="pgaptmp_004307"
     CDS             8564..9109
                     /gene="syd"
                     /locus_tag="ACHK0Z_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU4504221.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
BASE COUNT         2360 a         2252 c         2387 g         2183 t
ORIGIN      
        1 atttactgtg attagttgaa ccaggtcaca aaatataagc ttgtgaattt ctttctggtt
       61 aatcaggaag aagatggtgg gtaattaaac ggctaattca atagtgtgaa aggaacaaca
      121 ttattgccct gttttgaatc agagagaggt agctaatgga acgaaataaa cttgctcgtc
      181 agattattga cacttgcctg gaaatgaccc gcctgggact gaaccagggg acagcgggga
      241 acgtcagtgt acgttatcag gatgggatgc tgattacgcc tacaggcatt ccatatgaaa
      301 aactgacgga gtcgcatatt gtctttattg atggcaacgg taaacatgag gaaggaaagc
      361 tcccctcaag cgaatggcgt ttccatatgg cggcctatca aagcagaccg gatgccaacg
      421 cggttgttca caatcatgcc gttcattgca cggcagtttc cattcttaac cgatcgatcc
      481 ccgctattca ctacatgatt gcggcggctg gcggtaattc tattccttgc gcgccttatg
      541 cgacctttgg aacacgtgaa ctttctgaac atgttgcgca ggctctcaaa aatcgtaagg
      601 caactttgtt acaacatcat gggcttatcg cttgtgaggt gaatctggaa aaagcgttat
      661 ggctggcgca tgaagttgaa gtgctggcgc aactttacct gacgaccctg gcgattacgg
      721 acccggtgcc agtgctgagc gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct
      781 atgggttacg aattgaagag taatttcgta aagcaacaag gagaaggatg atggctaaca
      841 gaatgattct gaacgaaacg gcatggtttg gtcggggtgc tgttggggct ttaaccgatg
      901 aggtgaaacg ccgtggttat cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat
      961 gcggcgtggt ggcgaaagtg accgataaga tggatgctgc agggctggca tgggcgattt
     1021 acgacggcgt agtgcccaac ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc
     1081 agaatagcgg cgcggattac ctgatcgcta ttggtggtgg ttctccacag gatacttgta
     1141 aagcgattgg cattatcagc aacaacccgg agtttgccga tgtgcgtagc ctggaaggac
     1201 tttccccgac caataaaccc agtgtaccga ttctggcaat ccccaccaca gcaggcactg
     1261 cggcagaagt gaccattaac tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt
     1321 gcgttgatcc gcatgatatc ccgcaggtgg cgtttattga cgctgacatg atggatggta
     1381 tgcctccagc gctgaaagct gcgacgggtg tcgatgcgct cactcatgct attgaggggt
     1441 atattacccg tggcgcgtgg gcgctaaccg atgcactgca cattaaagcg attgaaatca
     1501 ttgctggggc gctgcgagga tcggttgctg gtgataagga tgccggagaa gaaatggcgc
     1561 tcgggcagta tgttgcgggt atgggcttct cgaatgttgg gttagggttg gtgcatggta
     1621 tggcgcatcc actgggcgcg ttttataaca ctccacacgg tgttgcaaac gccatcctgc
     1681 taccgcatgt catgcgctat aacgctgact ttaccggtga gaagtaccgc gatatcgcgc
     1741 gcgttatggg cgtgaaagtg gaaggtatga gcctggaaga ggcgcgtaat gccgctgttg
     1801 aagcggtgtt tgctctcaac cgtgatgtcg gtattccgcc acatttgcgt gatgttgggg
     1861 tacgcaagga agacattccg gcactggcgc aggcggcact ggatgatgtt tgtaccggtg
     1921 gcaacccgcg tgaagcaacg cttgaggata ttgtagagct ttaccatacc gcctggtaaa
     1981 tgcgctgatg tgataatgcc ggatacgacg tttgcggcgt atccggctcg ccgttaccgt
     2041 accaaccgca attgctgtaa attgccgtcg atatgcaaat cggtttgtaa gcgggcaata
     2101 tcgcggcaca gaaacgccat ctctttatgg gtttctaatt ttttgcgcca cttttcggca
     2161 accgcatcca gattctcata tatcccttcc agactctgaa actcgaccag cagctgcgtg
     2221 gcgctttttg gtccgattcc cgcaacaccc ggtacctttg aactgctgat ccccgcaagt
     2281 ccccagtaat cgggcaactg ctgcggttga acgccaaatt ctttatcgat aaatggcgca
     2341 tccagccaac gtttctggaa gtaatcacga atacgtaatg tcggtgaaag taactgacag
     2401 tagcctttat ctgtcgaaac aatcgttgcc tgatgcccgg cctgtgtcac tttgaccgcc
     2461 agcgtggcgg ctaagtcatc ggcttcgttg ccgctggttg accagcacgg gacgccgcgt
     2521 tgctcaaagg cggcgcgtaa tgcaggcatc tcgtcgtgca actcttccgg cattggcggt
     2581 cgacccgctt tgtaatctgg taaacgctga tgacgccagc cgctactgcg gttttcatca
     2641 tcaaaaacgg cgaccgcgtg ggttggctgg ctgtgcataa tgagctgatc gagcgcatgc
     2701 tgacaggttt cgacacaggg cgatccctga acggcatgaa tgcgacgaat aagattcagt
     2761 gcatcgacaa taagcaaatg aacagccacg ataaccccct taaagatcag gcgataaggg
     2821 taacattcga tgccggaaca ggctacggct ggatgagaaa tcgggaagag gcctcgcaaa
     2881 acgaggcctc tggagagcga ttaatcgcag gcaacgatct tcattgccag gccgccgcga
     2941 gaggtttcgc ggtacttggc gttcatatct ttacctgttt cgtacatggt ttcgataact
     3001 ttatcgaggc agacgcgcgg ctcgctggta cggcgtagcg ccatacgtgc ggcgttcacc
     3061 gcttttactg ccgcaatggc gttacgctcg atgcatggca cctggacctg tccggcgacc
     3121 gggtcacacg tcagaccgag gttgtgctcc atggcgattt ccgccgcgat gcacacctgc
     3181 gccgggcttg cacctaacag ttctgccaga ccagccgccg ccattgagca cgccacacca
     3241 acttcaccct ggcagccgac ttcagcacca gaaattgacg cgttcatctt ataaagagaa
     3301 ccaatggcac tggctaccag caggtaacga gccagtgagt tagcgttcac ttcgcggata
     3361 aacttgtcgt agtacgccag cactgccggg ataatcccgc acgcaccgtt agtcggcgca
     3421 gtcaccacgc gtccgccagc agcgttctct tcgttcactg ccagtgcaaa catgttgatc
     3481 cagtcaacaa ccgccatcgg gtcagtggtg gttttatcct ggctgaccag catccggcgt
     3541 agtgccgcag cacggcgtgg aacgcgcagt ttgccaggca acacgccttc ggtggaaata
     3601 ccgcgctcaa taccgccacg catgacttcc catacgttcg ccaggtgctg ttccagctct
     3661 tctttgctgt gcagcgccag ctcatttttc atcatcaggc cagagagtga cagcccggtt
     3721 tcctgacaat gtttttgcag atcggctgct gaactgtacg gataaggaac ttcaaccggt
     3781 gcgctattct gctggccaaa atgctcttca tcaacgataa agccaccgcc aatagagtag
     3841 taagtctggc tgtaaacgac tttatcgccc gccagcgcgg taatgcgcat accgttttca
     3901 tgcagagaaa ggttgtctgc atggaagttc atgcactgat caaccgggaa ctccacttca
     3961 tgctgaccgt ttgccagcat caggcgacca tgagtattca catcctgaat aaaaccaggg
     4021 atggaatcga tatccacggt atccggcagg ttacccgcca ggcccataat aatggcgata
     4081 tcagtgtggt ggcctttacc ggtcagagag agcgagccgt acacgtcaac caccacgcgg
     4141 gtcacgtctt taagcagatt acgggcaatc agatcgtcgg taaattgttt acccgctttc
     4201 attggtccaa cggtatgaga actggaaggg ccaatgccga ttttgaaaat atcgaatacg
     4261 ctaatcatag gaaatacatc gcgttaaaac ggaggaagcg ccgcccgaaa gcggcgcgaa
     4321 aggacttagc tgaacagaga gtagaagatt gcggagattg caatcagacc catcacgaca
     4381 acgaatacgt tgctgatgtg accgctgtac ttacgcattg ccggtacttt ctgaattgcg
     4441 tacatcggca tcaggaacag gatcatcgcg atgattgggc cgcccagggt ttcaatcata
     4501 cccaggatgc tcgggttcag ggtggcaaca atccaggtcg ttaccagcat gaacagcgca
     4561 gtgatacggt tcagcttgtt gatttcgata gacttacctt taccacgcag agatttaatc
     4621 accataccgt tgaagccttc acgtgcgccc aggtagtggc cgaggaagga tttggtgata
     4681 gcgataatcg cgataatcgg agccatccac gcgataaccg gtgcgttaaa gtggttagcc
     4741 aggtaagaca gaatcgagat gttctgctct ttagccgcag ccaggtctgc cggagtcagg
     4801 ctcagtacgc agctgaagac gaagaacatt acggtcagca ccatcatgat gtgtgcgaat
     4861 gcaaggatct tcgagcattt ctgttctgcc atatcgccgt actcttcacg cttcgcaacg
     4921 gcgaaagaag agatgatcgg agagtggttg aacgagaaca ccattaccgg aattgccagc
     4981 cacagggtca tccacagacc gttcccggtt gcagatgcag tgtccagaga cagcgtttcc
     5041 agtgctgcgc cgttccactg cgggatcagg tacagagcca gcagcatcag tacgccaaca
     5101 aacgggaata ccagaatact catcgcttta acgatcatct gctcaccaaa gcgaacgatg
     5161 gtcatcatac ccacgatcag gatcagcgac agaatcgcac gcggcggtgg cgtcataccc
     5221 agctggtgag acatgaagct ttcaactgta ttggtgattg ccacgctata aaccagcagg
     5281 atcgggtaga tagcgaagaa gtagagcagg gtaatcagtt tacctgcgcc aataccaaag
     5341 tgttcttcta caacctcggt gatgtcttcg cccgggtttt taccagacag tacgaagcga
     5401 gtcaggccgc ggtgagcaaa aaacgtcatc gggaacgcaa ggatagccat gatgatcagc
     5461 gggatcatac cgccaacacc ggcgttgatt ggcaggaaca gcacgcccgc gccgattgcc
     5521 gtgccgtaaa ggcccagcat ccacatggtg tctgtcttgc gccaggcact acgagagtct
     5581 ttcgacgcaa tcgtgctggt ttgagtcgtt tccatctatt tctcctggag gaatgcaaat
     5641 aattcagctt ttagccagtt cagcccggga aggtgcctga ccgtaaaaat gaaatctgtc
     5701 tatcggcgat ggcttaatca tttaccgccg taataatttg agggcggaaa gatacacaca
     5761 agttatgtat ctatcagtga tcttgatctc aaatacaata atctacttgg aagatagatt
     5821 atcccaacgc tttactctgt aaatcaaaag taaaagagaa tttaagggca atatttgccc
     5881 attaaacgac atctgacttc ttccggggcg cgcaatatat gtaggttgaa ctgaaaaaat
     5941 caatactttt agttaatcat ggcgataatt tctttttcat agcgataaaa tacggtttat
     6001 gaaaataggg aaacgtttgc gttgcaacat aaattgtatg acatattaac tgtgatgata
     6061 tttgtcgcgt gagggtggta agaaacattc atataacaaa tgaaatgagt tgatgagtaa
     6121 gtgaccaaaa acgagaaaac ccggcaacaa acgtcaccgg gggagcggag attacgtgca
     6181 gatttcgtag caagggatgt aggctgagcc tggcaacttc atacgatgct gggcaacaaa
     6241 accctgtagc aggtcgtcca tacgacgcat aatctcttta tcgccgttga ttttgtaagg
     6301 accaaactct tcaatggcgc gaataccgac ttcttttacg ttacccgcca caataccgga
     6361 gaacgcacgg cgcaggtcgg cagccagcac ttcaacaggt tgatccgggt aaagcttcag
     6421 attagccata ttctcgtgag acggctcaaa cggcatttgc aaatctggtg caatgcgcat
     6481 tgaccagtta aagctgtaag cgtcaccagt gtcgcggcga ttttctttca ccagcggcat
     6541 cgattttttc atctgacgag cgacttcagc ggcgtcatca atgatgatgc ggtaatggcg
     6601 gcgcgcgttt tcgcccagcg tatgtacgac aaactcgtcc agtacgcgga agtagtcggc
     6661 gctctctttc gggccggtga ggatcaatgg taaaacctga tctttgttgg ccgggttcat
     6721 taaaattccc agcaaataca gcaactcttc tgccgtaccc acaccgccag ggaagataat
     6781 gatgccgtga gcgatacgga caaacgcttc cagacgtttt tcgatatccg gcatgatgat
     6841 caattcgttg accagcgggt taggcggttc agcggcgata atcgacggct ctgtcatacc
     6901 aataaaacga ctgtctttgt aacgctgctg cgcgtgtccg accgcagcac ctttcatcgg
     6961 cgcttccatc gctcccggac cacagccggt gcagatattc agctcacgca ggcccagctg
     7021 gtttccgacg cgacgggcat acaaatactc attttcgtta attgagtgac cgccccagca
     7081 gaccaccata tttggcgctt cacccacatg cagcgcgcga gcgttacgca agatggaaaa
     7141 gaccaggtta gtgatgtgga cggagttgtc gagattcagg ttgggaaaac gaacggtgtt
     7201 atggatttgc ccgtaaacga agagaatgtc acgcaggacc gcgaacaagt tggcctgcaa
     7261 agcgcgaata attcgcccat cgacaaaagc ctcttccggg ggattaatca gttccagctt
     7321 tacgccgcgt tcacggcgca agacgttaat atcgaaattt tcaaaacgag acagcaattc
     7381 tttgctgtta tcggtcaaac taccggagtt cagtacggca agtgaacagt tgcgaaacag
     7441 ttgatagagg tcgctgctgg cggtgcgttt aagcatatcc acttccagct gcgacaacat
     7501 atccatggag ccaagcgggc taatatgtgt aatcaagaaa aactccttat gggacgaata
     7561 tcgtcattcc ctttgattac aatagccctg cctgatgacc tttcacaacc ttctgcgtga
     7621 atccagcacg caaaattgag aaaaaattta ttgccgaacc agtcttgtgg tcgatgggac
     7681 aaaatcgcta ttactgcgcc acgggttaat gtccagaccg ccacgacggg tataacgtgc
     7741 gtaaacgctc aatttttctg gctggcagaa gcgtaacagg tcattaaaga tgcgttccac
     7801 gcactgttcg tggaactcgt tgtgatgacg gaatgagacc aggtaacgca gcagtttttc
     7861 tctgtcaatt tggcgtccac gatactgaat ttggatcgaa ccccaatctg gttgatgggt
     7921 gatcaggcag tttgatttca gcaggtggct gacaagcgtc tcttccacta ctttttcacc
     7981 gctggtggca ttctccagat agtcagtagt gaattcatag ttatcgatag tgatatcctg
     8041 gtcatcaatg caggtgccat tgaaatgacc tatcggctgg ccttccagtt catcaagacg
     8101 atataacgcc acgctaacct taccctgggc gcaagtgctt aagtcgcgct ccagcgtctg
     8161 gcgcacctca tcccagttat taaaacgcgt ctggttaaaa ctgttgagat agagtttaaa
     8221 actcttcgac tcaatcagat ttacgctggt gtaatcaagt tcaacatgac cgacagcgac
     8281 ctgcggcaaa cctttcgcat tcagccagga aagttcatac agcgtccaga tatccgtacc
     8341 gtgaaaaggc aggttatccg ctttcagacc cagcgggtcg cgattcaggc tgcgtggaac
     8401 gccttgcagt aggctggcgt cataggtatc ccggtaatcg gttgattttc caagagtcag
     8461 gcccgcaagt gcctgatggt ttgcataaga agacatgttt caccgtcata aaccagataa
     8521 actaaacgac aagtgtatct ggtttaagtg aagagagaaa ttagtggacg atttgaccgc
     8581 acaagccctg aaagatttta ctgcacgcta ctgcgatgcg tggcatgaag agcataaaag
     8641 ctggccgtta agtgaggaac tgtacggtgt tccttcaccg tgcattattt caactaccga
     8701 agatgccgta tactggcaac cccagccgtt taccggggaa caaaatgtaa acgcggttga
     8761 acgcgctttt gatattgtga tacaacccac aattcatacc ttttatacga ctcagtttgc
     8821 cggggatatg cacgcgcagt ttggcgatat caaactgaca ttgctgcaaa cctggagtga
     8881 agacgacttc cgtcgggtgc aggaaaatct gattggtcat ctggtgactc agaaaaggct
     8941 aaaactgccg cccacgctat ttatcgcgac gctggaagaa gagttagagg tcatctcggt
     9001 gtgtaatctc tccggtgaag tgtgcaaaga gacgctggga acacgtaaac ggacgcattt
     9061 agcctcaaat cttgcggaat tccttaatca acttaagcct cttctgtaat ccaaataccc
     9121 catctggttg tgagagatct cttacagact ctgtaggaga tcgccaggaa ataagcgaat
     9181 ac
//
LOCUS       DAZFWP020000182         9179 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFWP020000182 DAZFWP020000000
VERSION     DAZFWP020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.182"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(154..4041)
                     /gene="purL"
                     /locus_tag="ACHK0Z_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /gene_synonym="purI"
     CDS             complement(154..4041)
                     /gene="purL"
                     /locus_tag="ACHK0Z_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU4504222.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4299..5855
                     /gene="mltF"
                     /locus_tag="ACHK0Z_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     CDS             4299..5855
                     /gene="mltF"
                     /locus_tag="ACHK0Z_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU4504223.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(5852..6355)
                     /gene="tadA"
                     /locus_tag="ACHK0Z_RS21550"
                     /old_locus_tag="pgaptmp_004310"
     CDS             complement(5852..6355)
                     /gene="tadA"
                     /locus_tag="ACHK0Z_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU4504224.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGAHDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(6413..7048)
                     /gene="yfhb"
                     /locus_tag="ACHK0Z_RS21555"
                     /old_locus_tag="pgaptmp_004311"
     CDS             complement(6413..7048)
                     /gene="yfhb"
                     /locus_tag="ACHK0Z_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU4504225.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            7257..8105
                     /locus_tag="ACHK0Z_RS21560"
                     /old_locus_tag="pgaptmp_004312"
     CDS             7257..8105
                     /locus_tag="ACHK0Z_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU4504226.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRQSEA
                     LMKKLV"
     gene            8420..8686
                     /locus_tag="ACHK0Z_RS21565"
                     /old_locus_tag="pgaptmp_004313"
     CDS             8420..8686
                     /locus_tag="ACHK0Z_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504227.1"
                     /translation="MRRKESNVSSLPELTNFEVSYSLVTNEVYLSASFTDNMACIPNW
                     PLQEFPDQLICISRAKAVALIEELQKTINYMDAGIDRSSGSLLQ"
     gene            8745..>9179
                     /locus_tag="ACHK0Z_RS21570"
                     /old_locus_tag="pgaptmp_004314"
     CDS             8745..>9179
                     /locus_tag="ACHK0Z_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504228.1"
                     /translation="MSEFSNLLNSIPGWLSSSLTALVGTLIGGWFTLKGVTQQAKLSK
                     VETERESLELQLSVLKGVKGEVFTLINLYNKRMKTHIDNIKPGQMLILTFPVGDDNFT
                     FYEQNANVIAKLNDSARDSIINIYTYSRSLIQSFKGNNKLIED"
BASE COUNT         2296 a         2457 c         2285 g         2141 t
ORIGIN      
        1 gtcgccagaa agcgacacgg taaaccaccc attgtcgtga tttacagaca caaaaaagct
       61 cccggagttg ggagcttatg atagtggttg gtgcttaatg ccggatgcgg cgtaaatgcc
      121 gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac gcgcattgcg
      181 gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg agttgctgac
      241 agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac tttcagtcgt
      301 aatcgacgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt cagtgacttt
      361 gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg
      421 cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg gcatttgcga
      481 gcccaccatc ccctgcaaca gcagcgacgg gctttgggtc acttcaacca ggctgaaacg
      541 cgcttcaaag cgatcggagg tattgcgcac aaaacgtggc cacaactcac tacctgggat
      601 cagttcgcgc aggttagaca tcatctggca accgttacat acccccagcg ccagcgtttg
      661 cggacggtgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggattgactt
      721 cgcccaacct tcaccggcac ccagcacatc accgtaggag aaaccaccgc acgcgaccag
      781 ggcgtggaaa tcttccaggc ccgtgcgtcc agtcagcagg tcactcatat gcacatcgat
      841 tgcatcgaag cctgcacggt ggaaagctgc cgccatttca acatgcgagt tcacgccctg
      901 ctcacgcagc acagcaactt tcggacgtgc gccagtggca atatacggtg ctgccacatc
      961 ttcgttgata tcgaacgaca gtttcacatt caggcccgga tcggcgtcgt ttgatttcgc
     1021 ctggtgctcc tggtcagcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt
     1081 ttctgcccac cagacacgca acgtggtgcg gctttcgctg aatacagtct gcccgttggc
     1141 ggtaatcaca aaacggtcac cggaaaccgc ctgccctaca taatggacac aatcagcaag
     1201 cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatcac
     1261 cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcgcccagag aggcaatatc
     1321 cgcgttaatg ccgcaatgac cagcaaaggc catttccgcc agcgttacca gcaggccgcc
     1381 atcagagcgg tcgtggtatg cgagcagctt acgctgtgca accagcgcct gaatcgcgtc
     1441 atagaagcct ttcagttgcg cgacatcgcg tacatctgcc ggtttgtcgc caagctgacg
     1501 ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag
     1561 cagcagtgcg ttatcttcgg tagaaagctg cggcgtgatg gtgtgacgta catcttccac
     1621 gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc
     1681 ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag
     1741 cgccggacaa agctcttcgc ccacggcttt aacggcttca tacaggcccg catcttcacc
     1801 agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat
     1861 ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa
     1921 atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc
     1981 gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggac ccaccatctg
     2041 atcgcgcgct accatgccag ttacgctgcg gtcaccgatg gtcaccagga aagttttctc
     2101 cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg
     2161 gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt
     2221 accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag
     2281 agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt
     2341 acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg
     2401 ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg
     2461 cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg cgttagaaag
     2521 accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa gctgccagca
     2581 acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct gtacggaagc
     2641 aaagtcgagg tcggcatcag actgacctga cgccatggaa gacgccgctc cgccgccaag
     2701 accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt tgatctcgcc
     2761 tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg
     2821 ataaccgcgc agctcttcgc cgttgtggct gttcactttt tcttcataag tacggaagta
     2881 gccattcagt gccggacgac caaattcgtt gttaaacgcc gcgccgccca gcgggccttc
     2941 ggtcatgatg tccagcgcgg tgacaatgcg ctcaggctta ccgaaatctt cttcccacgg
     3001 ctgttcgaag ccaggaattc gcaggttgga aacggagaaa ccaaccaaac ccgctttcgg
     3061 cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc
     3121 gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcagaatatg
     3181 cgccggttcc tgatggaaat cgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac
     3241 ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt aatctggcgt
     3301 ggtttcgaag gtgtttttga tcatcttaaa cagcgatttc ggctgttgct caccgtcgat
     3361 aacccaatcg gcgttaaaaa tcttgtggcg gcagtgctcg gagttcgcct gggcgaacat
     3421 atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc
     3481 aatttcatct tccgccagtg ccaggccaag acgcaggtta gcgtcgatta gcgcctgacg
     3541 gccctgcccc agcaaatcaa cgctggtgac cggagtcggt tgatggtgtg caaacagctg
     3601 ctctgcatca tctaaagcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt
     3661 aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg
     3721 ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg ctttcgaaga
     3781 ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg
     3841 gctggcgagt gccgggccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt
     3901 taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag
     3961 cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga
     4021 accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg
     4081 gggaaacggg cgtcattata aagaatctga tgcgctgacg aaaccgtttg cgtggaaata
     4141 aaatcaccat cgtgaattag caacgcgtgc cgccaatggc tgtaataagt tgccatctgg
     4201 cgcaggttta cgcaaaatgc cgctcattta tgagtaaacc tttcactatt attacgtttt
     4261 ttcaagctgg gacgcgcacg acacagagaa ttaactaatt gaaaaaatta aagattaatt
     4321 atctgttcat cggcattctg gcactgctgc tcgcggtcgc tctctggcca tccattccct
     4381 ggtttggtaa agccgacaac cgtatcgccg ccattcaagc gcggggagag ttgcgtgtga
     4441 gcaccattca tactcccctg acttataacg aaatcaacgg gaaacctttt ggcctggatt
     4501 acgaactggc gaaacagttt gccgattacc tcggcgtaaa actgaaagtg accgtgcggc
     4561 agaatatcag ccagctgttt gacgaccttg ataatggtaa cgccgacctg ctggcggcag
     4621 gactggtcta taacagtgag cgggtaaaaa attatcagcc tggccctacc tattattccg
     4681 tgtcacaaca actggtttat aaagtgggtc agtatcgccc acgtacgctg ggcaacctga
     4741 cggcggagca actcaccgtt gcaccgggtc atgtggtggt taacgatctc cagaccctga
     4801 aagaaacaaa attcccggaa ttaagctgga aggtagacga caaaaaaggc tctgcggaat
     4861 taatggaaga tgtcatcgaa ggaaaactcg attacaccat tgctgattct gtcgccatca
     4921 gcctgtttca gcgcgttcac ccggagctcg ccgtagcgct cgatatcacc gatgaacaac
     4981 cggtgacttg gtttagcccg ttagatggcg ataataccct ttccgccgcc ctgctcgact
     5041 tcttcaacga aatgaatgaa gacggtacgc tggcacgcat tgaagagaaa tacctggggc
     5101 atggcgatga ttttgattac gtcgatacgc gcacattttt acgcgccgtc gatgcggtac
     5161 tgccgcagtt aaagcccctg tttgagaaat acgccgaaga aattgactgg cgtttgctgg
     5221 ccgctattgc ttatcaggaa tcgcactggg atgcacaggc cacttcaccg acgggtgtgc
     5281 gcggcatgat gatgttaacc aaaaataccg cgcaaagcct cggcattacg gatcgtaccg
     5341 atgccgaaca gagcatcagc ggtggcgtgc gttatttgca ggatatgatg agtaaagtgc
     5401 cggaaagtgt gccggagaac gagcggatct ggtttgccct cgctgcgtac aatatgggct
     5461 atgcgcatat gctggatgcc cgcgccctga cggcaaaaac caaagggaat cctgacagtt
     5521 gggctgacgt aaaacagcgt ctgcctttac ttagccagaa accctattac agcaagctga
     5581 cttacggcta cgctcgtgga catgaagcct acgcttatgt cgaaaatatt cgtaagtatc
     5641 agattagcct ggtgggttat ctgcaagaga aagagaagca ggctacagaa gcggcgatgc
     5701 aactggcgca ggattatccg gcggtatcgc ctacggagtt gggcaaagag aaatttcctt
     5761 ttctctcgtt tctttcccag tcgtcatcaa actatttgac ccattctccc tctctgctgt
     5821 tttccaggaa agggagtgaa gagaaacaaa attaatccgt cgaggattgc gcttttttct
     5881 gcgctttaat ttcctggcgg cgcatgcgaa agaagtcact gagcaacgct gcgcactcat
     5941 ccgccagtat tccttccgta atttccactc ggtgattcat acccggatga tgcagcacat
     6001 ccattaaaga tcccgcagcg ccagttttcg cgtcatgcgc accaaagacc acgcgaccaa
     6061 tgcgactgtg gatcatcgct ccggcacaca ttacacatgg ttcaagcgtg acatacaacg
     6121 tggcgtcgat cagacgataa ttttgcatca ccagaccacc ctgccgcagg gccatgattt
     6181 ctgcatgtgc ggtgggatca tggcgaccaa tcgggcggtt ccagccttcg ccgattaccc
     6241 gattgttatg cactaatacc gcgccgaccg gcacttcccg ctcatcccag gcacgtttcg
     6301 ccagcgtcag cgcgtgacgc atccagtatt cgtggctaaa ttcgacttca gacaaaaaga
     6361 aaactccggt tataaaagcg cggcgcatta tacacggacg ctatgcttta ctctattcca
     6421 gttgctggag ttcaccgcgc ggggttactc gccaacgatg ctgacagaaa taaagcagcg
     6481 gattgtcctg attactgtcg ctatagccac tgtacagccg cagcggagtg ccgattttgc
     6541 gctccagttg tgcgaccttt tcatgtccca gacaacgcat cgtcaatacc caaccaccat
     6601 agccacgctg aatttggctg gcgataagat taacccgcgg cagccagggc gtatcgaaat
     6661 aaaccgcttc aaccagcggc tgcggagagc cggtaatcaa ccagatatca gcatcggaac
     6721 ttaacaggta ggtggttaat cgctcctgaa ccagcggaaa ggcggtaaca ttgtcgcgaa
     6781 accagcgcac gaaatcggcc tgcaacgtct gtaaacgtgc ttcgctgtga ccaaaagtgc
     6841 acccccacag aagcagactc atcggccagc gtgccgcacg accttttatc aataacgcaa
     6901 tggctataat cggtaacaac ggcaggacaa gtaacgcatt cagcggttgg cgacgcagta
     6961 aatagcgcag aaaactgccg aacatatcct gctgatgcaa tgttccatct aagtcaaaaa
     7021 acaccacacg acgctcgtga gttgccaaac catactcctc tggactgaaa catcctgatt
     7081 aattttcgct actacatagc ctaacagata gatcatcact tttccggcaa caatcgaccg
     7141 catcatggct aactggaatt tttaattcat ggcaattagc ggcaatggaa tataaaattc
     7201 actcgcgtgt gtctcatatt gcctgttgtc gccactattt catcgcgagg aaagatatga
     7261 acggcttact tcgtatccgt cagcgttacc aggggcttgc ccagagtgat aaaaaactgg
     7321 cggattatct gctgctacaa cctgatacgg cgcgccattt aagttctcag caactggcca
     7381 acgaagccgg agtcagtcag tccagcgtcg tgaagttcgc gcaaaaactc ggctataaag
     7441 gttttccggc gcttaagttg gcgttgagtg aagcgctggc aagccagccg gaatcgccct
     7501 ccgtgcccat tcataaccaa atccgcggtg atgatccgtt acggctggtc ggcgaaaaac
     7561 tgattaaaga aaatactgcc gctatgtacg caacgctaaa cgttaatagt gaagagaaac
     7621 tgcatgaatg cgtaacaatg ttgcgctctg cgcggcggat aattctgacc ggtattggcg
     7681 cttcgggtct ggtggcgcaa aactttgcct ggaagctgat gaagattggc ttcaatgctg
     7741 ccgcagtgcg cgatatgcat gcgctactcg caacagtaca ggcgtcgtcc cctgacgatc
     7801 tgttattagc catttcctac accggtgtac gacgcgagtt aaacctggcg gcagatgaga
     7861 tgctgcgagt gggcggaaaa gtgctggcga ttaccggctt tactcccaat gccctgcaac
     7921 agcgtgcttc tcattgcctg tataccattg ccgaagaaca agcgacaaac agtgcttcaa
     7981 tctctgcttg tcacgctcag ggaatgttaa cggatttgct gttcattgcg ctgattcagc
     8041 aggatctgga actggcacca gaacgtattc gtcagagtga agcgctgatg aaaaaactgg
     8101 tctgagtaaa gaatgcgcgt ataatgcccg cccggtttgt gttgttttga gagtttcctt
     8161 atggcgttgt taatcactaa aaaatgcatc aattgtgata tgtgtgaaat caaataaaaa
     8221 cacatttatt ttcatagtga tacagcaatg tgttattgaa tgtgttgttg gatttatggt
     8281 gcaaaaaaat tttgcataag ttcagcaaca caaagatccc cccccttttt ctataacggt
     8341 tcatttgaga ctcccccttg actccctctt cgaaattgaa gaccatcacc tgtacataat
     8401 tacagtcatg gataatatta tgcgcagaaa agagtcaaac gttagcagtt tgccggaact
     8461 caccaatttt gaggtaagct attctctggt taccaatgaa gtttacttgt ctgcatcctt
     8521 cactgataac atggcctgta taccaaactg gcccttacaa gaatttccgg atcaactaat
     8581 atgcatttct cgagcaaaag ctgttgccct aatcgaagag ctccagaaga ccatcaacta
     8641 tatggatgca ggaatagatc gaagttcagg tagccttctc cagtagctga cgagttttat
     8701 aataatttga tatatatatc gttcaatatt agcgagtact tataatgagt gaatttagta
     8761 atcttttaaa tagcatacct ggatggttat cctcctccct cacagctcta gttggtactc
     8821 ttattggcgg atggtttacc ctaaaaggag ttacccaaca agctaagctg tctaaagtag
     8881 agacagaaag agaatccctt gaattacaac tttcagtatt aaaaggagtt aagggagaag
     8941 ttttcaccct aatcaattta tataataaaa ggatgaaaac acacattgat aatataaaac
     9001 cgggacaaat gttgattctc acattccctg taggggatga taatttcaca ttctatgagc
     9061 aaaatgcgaa tgtcattgca aaactaaatg attctgctag agactcaata attaacatat
     9121 acacatattc acgctcatta atacaatcat ttaaaggaaa caacaaactc attgaggat
//
LOCUS       DAZFWP020000183         9102 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFWP020000183 DAZFWP020000000
VERSION     DAZFWP020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.183"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHK0Z_RS21575"
                     /old_locus_tag="pgaptmp_004315"
     CDS             complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHK0Z_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4504229.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            1758..1833
                     /locus_tag="ACHK0Z_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     tRNA            1758..1833
                     /locus_tag="ACHK0Z_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1791..1793,aa:Asn,seq:gtt)
     gene            complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHK0Z_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHK0Z_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU4504230.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2923..3840)
                     /gene="nac"
                     /locus_tag="ACHK0Z_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             complement(2923..3840)
                     /gene="nac"
                     /locus_tag="ACHK0Z_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU4504231.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4167..4242
                     /locus_tag="ACHK0Z_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     tRNA            4167..4242
                     /locus_tag="ACHK0Z_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4200..4202,aa:Asn,seq:gtt)
     gene            complement(4297..5232)
                     /gene="ldtA"
                     /locus_tag="ACHK0Z_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             complement(4297..5232)
                     /gene="ldtA"
                     /locus_tag="ACHK0Z_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4504232.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5294..6373)
                     /gene="cobT"
                     /locus_tag="ACHK0Z_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             complement(5294..6373)
                     /gene="cobT"
                     /locus_tag="ACHK0Z_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU4504233.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6385..7128)
                     /gene="cobS"
                     /locus_tag="ACHK0Z_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             complement(6385..7128)
                     /gene="cobS"
                     /locus_tag="ACHK0Z_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU4504234.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7125..7670)
                     /gene="cobU"
                     /locus_tag="ACHK0Z_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             complement(7125..7670)
                     /gene="cobU"
                     /locus_tag="ACHK0Z_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU4504235.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7822..7995)
                     /locus_tag="ACHK0Z_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /pseudo
     CDS             complement(7822..7995)
                     /locus_tag="ACHK0Z_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7974..8162)
                     /locus_tag="ACHK0Z_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /pseudo
     CDS             complement(7974..8162)
                     /locus_tag="ACHK0Z_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2524 a         2422 c         1930 g         2226 t
ORIGIN      
        1 tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc ccacccataa tgtcaaagcc
       61 tgttttttaa atttgaaatc gtttgctgaa ataatctgca ttttgtcgtt tattccgaca
      121 cagctggctt tttttcacac ttttgcggct cggatcgagg gtatttccat agccaacgtc
      181 cagtaaccat tcgccagtaa aacagcacgg cccgcacagc ccagtcggca aacattccca
      241 tccagacacc aaccacaccc cagccaagca tgatgcccag cacataaccg acgacaaccc
      301 ggcaacccca catgctcaac atcgaaaccc acatggcgta acgggcatca cgagcacctt
      361 taaatccagc gggtagcacc catgaggcgg accaaatagg cataaataaa gcatttagcc
      421 aaatcagaat cacaacgaca tgtttaacct gtggatcctg ggtgtaaaac gatgccataa
      481 gcccggcaaa gggagccgtt agccaggcga tggccgttaa tccaagagtg gacagccaga
      541 acacatgccg caactgaatc tctgcttgcg ctatctgccc tacccccaac cttcggcctg
      601 taatgatcgt agaagcagag ccgagcgcac ttccgggtaa gttgataaga gccgcaattg
      661 aaaacgcgat aaaatttccg gcaataacac tggtccccat cccggcaacg aacatttggg
      721 ttaataaccg accactggta aataacactg attcgacact cgctggaata ccaatcccca
      781 tgacttccca gataatgcta aaattcagcg gtttaaaata gctctttaac gaaatcctta
      841 gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc aactgcgcca atataacgag
      901 aaatggttaa acccaaccca gccccgacaa atcccagtcc cggccaggag aaaaggccgt
      961 aaatcaatat gccgctaata ataatattaa gaatattcag gctaccgtta atcagtagcg
     1021 gtattttcgt attccctgca ccacgaagtg ccccgctacc aatcaaagtg atggcagctg
     1081 ctggataact gagtaccgtc agctccagat aagtcaacgc cagtgcttta acttctgtcg
     1141 tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa atgatgaata agcgttgcca
     1201 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
     1261 gtcgatcccg cttaccgaga ctaaatgcca caacgacagt agtaccaaga tcgatagctg
     1321 caaaaaaagc cataatgacc atattgaagc tgtccgccaa tcccacgccg gccatcgcat
     1381 cttttcccag ccagctgacc agaaaagtgc tcaggacacc catcaacagg acacaggcat
     1441 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ccagaacaac actttgtagc
     1501 tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
     1561 tcaaagccga ccttaattgc agaaagtgaa accacatttc aaataatgag ggagaatcag
     1621 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
     1681 acggcaaaca attggggaat ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga
     1741 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     1801 atgtcactgg ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg cttttatagc
     1861 gggctttttg ctatatctga taatcaattt cctcttcact gttttccatc acctgccgct
     1921 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccag acatagttac
     1981 gctgaagttg tcctcgcttc agtcccaacc agacagtatt agcatcgaaa agatgccgcg
     2041 tatccagacg gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc
     2101 cgatcccaag cccaagagca acataggttt taatgacatc agaatcctgc gcacttaata
     2161 caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc aatacgtgag cgccccgtaa
     2221 tcccctgtcg gtaagtgatt aacggccact tcgctattga ttccagcgtc aatggtgaaa
     2281 tttgcgtcaa gggatgatcg tgtggaacaa gcaaactatg gtgccaacga aaccacggga
     2341 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     2401 cattttgcaa caatgtcgca atttcctgtg gcgtcccctg gattagctcg agccgaacct
     2461 ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa gctataacgt gcctgagtat
     2521 gcgtcgttgc aatagtgaga acgccagacg tatcgttggt aaacaggtct gcaagccgac
     2581 gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     2641 cagtcatgcc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca agttcatcct
     2701 ccagttcccg aatatgacgg ctgacgcctg actgtgaggt aaaaagcata ttcgcaacct
     2761 ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca
     2821 cggtaaactc cgggcagttc agatttcccg ttattgttaa agtctaatgc ccggcataac
     2881 aaataataaa aacccgcatc ttattccatc ccgatataac acttagctca ccaattgcca
     2941 ctgccttttt tccatcactg gagaactaat cactgacatt aacaactctt tcactgcctg
     3001 tgcctgtggc gataagttcg ctctggcggg taaatttaat gacaaagaga gactcatgga
     3061 aggagtggta atgcgtgaca tccacccatt tactgcgcca cataacgaac gcgcggccga
     3121 ttcgggtaat actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg tggcaataga
     3181 ctcaatttca ccaataactt ttgccgtgag tcgccgtagg gaaaaagcct catcaacacg
     3241 aagtctaata gcactgtaat cactggggag aaagaggttc attttcgcaa tagcattcac
     3301 atcaacgctt tgccccgggc aatcttgagt tcctaccaga aaaagatctt ctttcagcaa
     3361 agcctgactg gatacaccag ccacagggga atgctcataa atcaccgcca tatcgagttg
     3421 gtgatttatc aatttttcgt taagcactgc accactattt tcatgaagat agataacgat
     3481 ctccggaaat tcagcgcgaa ccgcctgtaa taagggcatg gtgatggatg acgcagcggt
     3541 tcctggtgca aagccaatcg agacttgccc cgataatgcc tgaccaacgt tatgcaccgc
     3601 cagttgggcc tgttcacact gacgtaaaat ggcccgcgca tgggtataga gaatttttcc
     3661 ggcgtctgtt ggcgtaacgc cccgctttgt acggatcaaa agttgttgat ttaactcacc
     3721 ttccagtgtg gcaacctgct ggctgagcgc tggttgtgcg atatgcaata cttcagcagc
     3781 ctgggtcagg ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat
     3841 gttgcctccg gtttttaaga atcggcccaa gtgccgctat tacttacaac cagattgcaa
     3901 gatgcttgcc agttttattt tggcgttgat gtacaagcta accaactgtc aaataagaga
     3961 ttatgataga ttcgtcattt gctcctttaa tcagctgtcg cgttcccctg ccctataaaa
     4021 ggagggtatg caccacgatg gttcattacc caataagatt gaaagctcac cactttgttg
     4081 aaattgacag caaacaaaca aaaaaatgca tttcaccctt tgacatcacc atgcactgcc
     4141 attaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa cggcggactg
     4201 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattcaaa aaagcctgct
     4261 ttctagcagg cttttgcttt ctaattacca acgctcttaa aacatctgtc ttgaaccaga
     4321 actaatttgc acaggcattc ccgatcgacg ttgcaacgca gcatttgcgc gatttacatc
     4381 aacttcttgc ccgctgataa acgcccgcaa agatggggtt accggcaatg gcacttttcg
     4441 gtcagactca tattctgcac gattgcgcga caacggctca tgaacttcca gccagttcga
     4501 gccatctggt tcagtggtgt attttactgg ctggtcgata atttgcacac gcgtcccaac
     4561 aggaacatta tcaaacagat atttgatatc gtcattgcgc agacgaatac agccctgact
     4621 tacccggagc ccaataccaa aattggcatt ggtaccatgg atggcataca acctgccaat
     4681 ataaatcgcg tacagcccca tgggattatc ggggcccgca ggaacaaatg cgggcaaact
     4741 ctcccctcgt ttcgcatatt cgcgccgagt gttcggcgtt ggcgtccagg ttggagcttc
     4801 ttgtttacgt tcaacggtag tcacccagtt acgcggggtt tctcgcccag cctggccgat
     4861 accaatagga aagacttcca cagtattact gtctggtggg tagtaataaa gacgcatctc
     4921 agcgacgtta accacaatcc ctttacgaac agtggcgggc aaaatcagtt gctgcggaat
     4981 ggtgagttgc gagccagact tcggcaaaaa aacatcagcg cccgggttcg cttccagcat
     5041 gttacttaac ccttgcccgt attgtgcggc aaaagtctcc agcggctggg tattgtgatc
     5101 aggaacagtt acagtaaacg actgccccac taaacggcta ccctctggag gtaatggata
     5161 agttacagcc aggctagtat ggctggcaaa aagcagagca aatgagcaaa aaatatttac
     5221 acgacgcatc atgtcccttt cctaagtcgc gaaagctatc cgttaagtat agcttttatc
     5281 agacttttcg tttttaactg ttcaaatcag aagtcgtatt ccccggtaga acaatattac
     5341 tggcagcaag ttcgcccatg ttgttatata tcgcacaggc agcttcgatg atgggcatcg
     5401 ccagagctgc gccactcccc tcacctaaac gcatctccat attgagataa ggctccagcc
     5461 ccaaatgcga cagcgcaata cgcgcccctt tttctgccga caggtgagaa ggaatgagat
     5521 acggtttgat tgcaggagac atctggcagg ctgcgagcgc ggcagcataa gaaagaaatc
     5581 catccagcaa cacgggtaaa ccgcaggaag cagcgcctaa catcacgcca gccattccga
     5641 ccaaatcaaa tccacccact tttgccagga catcaacgcc atcctgcgga tttggttgat
     5701 tcaacgtaat cgcccggcgc acaacatcaa ttttattagc cagtttatct gtcggcaggt
     5761 ttgcgccaat cccaaccact tcttcaggag cccggccagt gattgtgctg actattgccg
     5821 ctgccggtgt cgtgtttgcc atccccagtt cacctacacc aaacagcgta acaccgtttt
     5881 ttgccagctc ccgcgtataa catatgacgt ccaaaagcaa cttttctgcc tgatggcgac
     5941 tcattgccgg agctgaagca atattgccac tacctcgagc aacacgcatg ttgataagcc
     6001 cggggatagg ctcagcagta tcaataccaa catcaattac gtagacgtta gcgcccgctt
     6061 gtgctgccag cacacacacg ccggttgttc cgcgggtcat attttcagcc tgaatggctg
     6121 tcacttcttt tggggaaata gcgacccctt cctcccagac gccgtgatcg gcacacataa
     6181 ccagtaccgc ttttttgccc acatgcggta tgccattcaa ccccggcatt cctgccagtt
     6241 gtatggcaag cgcctccagc cttcccaggc taccaacagg tttgagtaac ccgtcaatat
     6301 gccgttgtgc acgcgacatc gcggcaggat cgatggcagg gatcgtattc agtaaatcgg
     6361 caagtgtttg catgtcacgt ctctttatag cagtgccagc aggaagacca gttcaccaag
     6421 ctctatagca gctcccagcg tatcgccggt ttgcccgccc agcgtacgtt tgagaagctg
     6481 accgaggatg aaaatcgcca ccatcgtcac caccatagca gccacaccat gcatacccgg
     6541 caataatacg gcagcaaaaa tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc
     6601 aattttgcca atgaacacat tgccaagccc ttcttcccgg gcataacgat gacgatacat
     6661 caataatgcg gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg caaggatcgg
     6721 ttcgccacgc agagccaact cgcttaacac cagaatcttt gccagtacca caaaaatcaa
     6781 tgccagaccg ccgtgggtgc ctaaacggct atcacgcatg atttccaaca tgcgatcgcg
     6841 gctacgtgca gaaaatacgc catcacaggt atcagcgagg ccgtccagat ggaatccccc
     6901 ggtcatcagc accagcacaa gtacgctaaa cagtgccgcc agtggtgcgc cacaccatgc
     6961 ctgcagcacc atgaagacca gcccgctaat cgcgccaagc aataatccaa tcaaaggaaa
     7021 agtaataata ccgcgagaat aatgctcgaa atccagtccc tgggaccagc gacgcggtac
     7081 aggcagacgc gtaataaatg agagcatcgc ccaaaataat ttactcattt gatttttact
     7141 ccaatacccg aaaccaccag ccatacttca tttgccgcag cggccaactg ctgatttacc
     7201 cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct ccggcacaat ccccattccc
     7261 acttcgttag tcactaatac aacctttgcg gggcaacgtt ggcaggcagc aatcaacgac
     7321 tgaatctcag cattaatcac ctgttccatc gcctgataat cccattcatc agggtcttta
     7381 tcgccgccat aatcaaacaa cagattagtc accattgtgg taacgcattc aagcaacaca
     7441 acctcattcg ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca gcgctccact
     7501 gtgcgccagt gctccgggcg gccttgccga tgatgttcta tccgtgcagc catctcatca
     7561 tcaaggattt gcgaggtagc gatatacaga acctgtgaag agtccccaat aagcgcctct
     7621 gcgtggcgac tcttcccgct ccgtgcgccc cccgtcacca aaatcatcat accgactcct
     7681 gattcaaatg tatgatggta ttcattgaac agaacaatgg atcccccacc gttgctgacc
     7741 gcaaaacatt tacgcgatta atactgcgcg taatataatt aaaaatccag cattctcaat
     7801 ccattacgaa ataagatggc attacggaat agccagactt tctcgtgtta acgtccgaca
     7861 aaatgcgtca ttgacagcgg tttctgtgga ttgacacatt tcattcagat aatgaattaa
     7921 tgctactgcc ggaacaacca gcaaacgggc attgtgttct gaaatccagg ctattgattc
     7981 aacgtcagcg acagatctgc gctgtaaaag aacttgtaaa acaacgtaat agaattatcc
     8041 ggaatggtgg cgactatgca ctagaacacc acaatttcgc tctctcggta aggcactgtc
     8101 acagcattca acagaatgtg acttgccaga tttattagcg ccaccagatg tttaaccggg
     8161 taaccacgac ccagtcgggg atatattcca tcttctcgct gacatcatca agaataaaaa
     8221 ggtttatcac actcagagca acgttcttca gcaggcggga taattcttca acatttagaa
     8281 gatgtgttat taaaggcata caaactggac gtattgtatt ttcttttggt ggttgaccta
     8341 aggtagcagt ttatcctgat gcgctgagat ttctgtaata tctacgtcaa cattctcctc
     8401 gaatagaaaa tgcagccccg gcaagtgatt cacatttttt aacaaaacgt tgttagcgtg
     8461 ccattttcag aagctctcca agaaagcgaa ttcttttctc aaattctgca ttctcagaca
     8521 cagctttcta ctccatggta atggcccaaa tacgaagttg ctcaggatca ttagtggtgc
     8581 tgagaagtta aataaccatg cggagtacca ctcgtcatca cgtatttata tatcatgaag
     8641 ctattgttgt gttttgtaca tcagtagata tattctgata tactcctttt gctagacata
     8701 acctttcacc tgcttgcaaa gcttctgtgt tctgacattg ccaaattgtt gcaattctgt
     8761 atccagcctt ctttcagtca tagcttcggg ccgcgataag actcactgat ctgaccctga
     8821 ttcctcttgc agactttata gaccaattaa aatgcagttt ctgcaggtca acgtctgacc
     8881 atcattgtca tcactctggc cattagagta accttctgca ttcatccttt tgtaaaaagt
     8941 ttatattagt atcagcaatt aaccggacct gatactgata tgagtcttac cgcatatacg
     9001 gtcaatttca gcaattaatt acattatcca cgccaaagta tttgtcatca caatgatggt
     9061 accttctttc agacaccatt ttttcaactc cgttttccac gg
//
LOCUS       DAZFWP020000184         9031 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFWP020000184 DAZFWP020000000
VERSION     DAZFWP020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.184"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="ACHK0Z_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="ACHK0Z_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU4504236.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="ACHK0Z_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="ACHK0Z_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU4504237.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="ACHK0Z_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="ACHK0Z_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU4504238.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="ACHK0Z_RS21645"
                     /old_locus_tag="pgaptmp_004329"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="ACHK0Z_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU4504239.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTHPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3241..4635
                     /gene="ychO"
                     /locus_tag="ACHK0Z_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /gene_synonym="iatA"
     CDS             3241..4635
                     /gene="ychO"
                     /locus_tag="ACHK0Z_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU4504240.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4636..5286)
                     /gene="narL"
                     /locus_tag="ACHK0Z_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             complement(4636..5286)
                     /gene="narL"
                     /locus_tag="ACHK0Z_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU4504241.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5279..7075)
                     /gene="narX"
                     /locus_tag="ACHK0Z_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             complement(5279..7075)
                     /gene="narX"
                     /locus_tag="ACHK0Z_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU4504242.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7414..8805
                     /gene="narK"
                     /locus_tag="ACHK0Z_RS21665"
                     /old_locus_tag="pgaptmp_004333"
     CDS             7414..8805
                     /gene="narK"
                     /locus_tag="ACHK0Z_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU4504243.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2199 a         2192 c         2325 g         2315 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagcgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcatcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttatcc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgggcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc tcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat aagaggatgt gtgctaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catcaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacattca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt ttttcattat taccggaagt taccgacgtt
     3241 ttgagccgtt tcgttcctcg cattatcccg ttttatttac tcttgcttgt agcaggcggt
     3301 acagctaacg cacaatctac cttcgagcaa aaagcggcaa atccctttga taataacaat
     3361 gatggtctgc cggatttagg catggctccc gaaaatcatg atggggaaaa acactttgct
     3421 gaaattgtga aagatttcgg cgaaaccagt atgaatgata acgggctgga tactggcgag
     3481 caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc ttagtcaaca ggttaatcag
     3541 cacgtagagt cctggctatc accgtgggga aatgccagtg ttgacgtcaa agtggataac
     3601 gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc cgttacaaga taatgatcgt
     3661 tatctcacct ggagccagct tggtcttact cagcaggatg atgggttggt gagcaatgtg
     3721 ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg gttataacac tttttatgac
     3781 aacttgctgg acgaaaatct tcagcgagcg ggctttggtg ccgaagcgtg gggcgaatat
     3841 ttgcgactat cggcaaactt ttatcagccg tttgctgcat ggcatgaaca gacagccacg
     3901 caggaacaac ggatggcgcg cgggtatgac ctgacagccc ggatgcgcat gccgttctat
     3961 caacacctca ataccagtgt cagcgtagaa cagtattttg gtgatcgtgt tgatttgttt
     4021 aactctggta cgggttatca caatcccgtc gcgttgagtc tgggattaaa ttacacccct
     4081 gtgccattag tcactgtgac ggcccagcat aaacagggtg aaagtggcga gaatcaaaat
     4141 aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc tcaaaaaaca actttctgcg
     4201 ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct atgacaatcc gcagcgaaat
     4261 aatctaccga ctcttgagta ccgacagcga aaaacgttaa cggtgtttct ggcgacaccg
     4321 ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat tacaaatccg cagtcgttac
     4381 ggtattcggc aactgatttg gcagggcgat acgcagatat taagtttgac gccgggcgca
     4441 caagccaaca gcgcggaggg ctggacgctg atcatgcctg actggcagaa cggggaaggg
     4501 gcaagcaatc actggcgatt atcggtggtg gtggaagata accaggggca gcgtgtctcc
     4561 tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg cattgtcaaa cgacgaactg
     4621 cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc cataccgctg cttccacgcg
     4681 agacttgagc ttcattttct tcagcatgtg cttgacgtgc acttttactg tgctttcggt
     4741 gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc tgggcaatca gcttgagaat
     4801 atcgcgctcg cgtggggtta actggttaac atcgcgctca gtagtggcac ggttagcgcg
     4861 caagctggcg gccagaacag gcgttaatgc ttcgcttaat accatttcgc cagcagctgc
     4921 ctgatgcaat gctttcagca gatcttccgg ttccatatct tttaacagat agccatccgc
     4981 gccgcgtttc agtgcggtga ccacatcttc ttcatggtta gagacgctga ataccacaat
     5041 gcgccctgag agggactttt cgcgcagttt atccagcgtt tccagaccgt tcatgccggg
     5101 catattgaga tctaacagga tcagatcggg atcaagagac tccgccagtt caataccctg
     5161 ttcgccatta ctcgcttcgc caaccacggt gatatctggt gccatactga taagctgttt
     5221 tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg atagtagccg gttcctgatt
     5281 actcatgggt atctccttgg acgtctgtga aagttttttc gggaataaag gtgaccacca
     5341 cttcggtgcc acctgattca cgacggcgga cgcggcaatc gcctcgtaaa ctttgcgcac
     5401 ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat ggcattttca ggcacgccgc
     5461 agccattatc ctgaacggtc agttttacct gattatcgtt ttgcgccacc gtcaccacga
     5521 cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc ctcacgggca atttgcaaca
     5581 agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa ttgataatcc agcttcaccg
     5641 ggaagccaaa tttggcgctg tactcttcgc aactcgcctc cagcgccgga cgtaatccag
     5701 gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg caactgcgcc caggatgcat
     5761 tcagttcgtt acggatctga cttaacagtt cgcggctgct ttctggcagc gcatcgccct
     5821 gcatctgtaa acaactcacc tgcatcttca tgcaagagag agattgggca atagaatcat
     5881 gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat caactgttgc tgacgttcct
     5941 gatggcgatc cagcgccagc gtggcggtga gttgttcaac cagggtatcc accagttgtt
     6001 gttgatcatg gctaagatga cgcccctgcg gcagggtcgc cagcaaaata ccgtactgcg
     6061 tatgagagtc agccagccgc cacttcaggg tcgtgccgcg atcgccaacg ggtaatacgc
     6121 cgcgcgggca gagctggcag cctttatcat cacaagtcat atctggctgg caggtaaact
     6181 cctgatgatt ctcttcatcg tcagtatcat aaacccgcag ttcgatatca cgtagcaggg
     6241 ttaaattctg taagccgttg agtacaggtg acaggcgttc acacagcggg gcgcgggaat
     6301 gcaaacggcg gttagcctgc cataaaaaag agaggatctg atttttatgc tccagcccgg
     6361 cggttttctc ctgaacccgc tgctcaagta cggcataact ttcggccagt tctgcagaca
     6421 tattgttcaa tgcagttcca agcatcgcca tttcgttgcg cccgctgatg ttcgcgcgtt
     6481 gggtaaaatc gcgatggctg acggcactcg ccattgccag cagttgccgc cacggttgta
     6541 gcagtcgcgc ccgcaaccaa ataatagtga acaccagtaa aagtgccata aataccgcca
     6601 ttacccgatg gaccagtacc actgtctcaa tgcgcatttc cgtggtgcgg tcaaaaccag
     6661 ataccagctg atcaagcccg gcaacaaact ggctgacatc cgctgacacc gtttcgcggt
     6721 tttgtgcacg tatcagcgca gggatcagtt cattacgcca gtaatcttgt aaaccctgta
     6781 attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa ctcggcgcta aatgccgttt
     6841 gttccatctc tttaattaag ggcttgtctt tctcgcttaa tggcactgct gccaacagac
     6901 ggtaactttg catgcgcagc gatcccgctt tgttgatcgc atgggcgctg ccctgaacgc
     6961 cttgcaccag ccagccagaa accgccatcc ctgccagtcc aatagcagta gaaagcaaca
     7021 caataagcgc aacctgatta accagggtga gcggagagag acaacgtttg agcatgtaaa
     7081 cctcttcctt caggctttaa atgagcaata accttaatga atgtgacgat acattctgga
     7141 atggcagtat tctcggctat tggctgaagt atacccatac ccggaaggag ttactcctta
     7201 tttgccgtgt ggttagtcgc tttacatcgg taagggtagg gattttacag caccgtgaaa
     7261 aatctcataa tttttatgaa atcactgtac tcactatggg taatgataaa tatcaatgat
     7321 agataaagtt atcttatcgt ttgatttaca tcaaattgcc tttagctaca gacactaagg
     7381 tggcagacat cgaaacgagt atcagaggtg tctatgagtc actcatccgc ccccgaaagg
     7441 gctactggag ctgtcattac agattggcga ccggaagatc ctgcgttctg gcaacaacgc
     7501 ggtcaacgta ttgccagccg caacctgtgg atttccgttc cctgtctgct gctggcgttt
     7561 tgtgtatgga tgttgttcag cgctgttgcg gtgaacttac cgaaagtcgg ttttaatttt
     7621 acgaccgatc agctatttat gttgactgcg ctgccttcag tttctggcgc gttattacgt
     7681 gttccatact cctttatggt tcctatcttc ggtggtcgtc gctggacggc gttcagcacc
     7741 ggtattctga ttattccttg tgtctggctg ggttttgccg tgcaggatac ctccacgcct
     7801 tatagcgtct tcatcatcat ctctctgctg tgcggctttg ctggcgcgaa cttcgcctcc
     7861 agtatggcaa acatcagctt cttctttccg aaacagaagc agggtggcgc gctgggtctg
     7921 aatggtggtc tgggcaacat gggcgtcagc gtcatgcagt tggttgctcc gctggtggta
     7981 tcactgtcga ttttcgcagt atttggtagc cagggcgtca aacagccgga tgggactgag
     8041 ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc ttgccatctt caccattgcg
     8101 gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct ccatcaagga gcagttgccg
     8161 gtactcaaac gggggcatct gtggattatg agcctgctgt atctggcaac cttcggctcc
     8221 tttatcggct tctccgcggg ctttgcgatg ctgtcaaaaa cgcagttccc ggatgttcag
     8281 attctgcaat acgctttctt cgggccgttt attggtgcgc tggcgcgttc tgcaggtggt
     8341 gcattatctg accgtctggg cggaactcgt gtcacgctgg tgaactttat tctgatggcg
     8401 attttcagcg gcctgctgtt cctgacctta ccgactgacg ggcagggcgg aagctttatg
     8461 gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg ggctgggtag tggttccact
     8521 ttccagatga tttcagtgat cttccgtaaa ctgacaatgg atcgcgtgaa agcagaaggg
     8581 ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca cggcggcggc gttgggtttc
     8641 atctctgcga ttggcgcgat tggtggcttc tttatcccga aagcgtttgg tagctcgctg
     8701 gcattaacgg gttcgccagt cggcgcaatg aaagtatttt tgattttcta tatcgcctgc
     8761 gtagtgatta cctgggcggt atatggtcgg cattctaaaa aataaaccgt tactcgtcat
     8821 acttcgggtt acatgtgctg cggctgcgtt cattcacccc agtcacttac tttagtaagc
     8881 tcctgggatt cattcacttg ccgccttcct gtaaaccgaa ttatatagag taaaatattt
     8941 gattatcctt tgcgcggcat gatgtcgcgc tttttttatg cgtcatttag ttacaacata
     9001 ctaatgttgt atggtttatt tcgccggatt t
//
LOCUS       DAZFWP020000185         8860 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFWP020000185 DAZFWP020000000
VERSION     DAZFWP020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.185"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..881)
                     /locus_tag="ACHK0Z_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             complement(<1..881)
                     /locus_tag="ACHK0Z_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4504244.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQG"
     gene            complement(883..1155)
                     /locus_tag="ACHK0Z_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             complement(883..1155)
                     /locus_tag="ACHK0Z_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504245.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            1291..1548
                     /locus_tag="ACHK0Z_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             1291..1548
                     /locus_tag="ACHK0Z_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4504246.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            1554..1853
                     /locus_tag="ACHK0Z_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             1554..1853
                     /locus_tag="ACHK0Z_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4504247.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(2058..2402)
                     /locus_tag="ACHK0Z_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             complement(2058..2402)
                     /locus_tag="ACHK0Z_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504248.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(2399..2764)
                     /locus_tag="ACHK0Z_RS21695"
                     /old_locus_tag="pgaptmp_004339"
     CDS             complement(2399..2764)
                     /locus_tag="ACHK0Z_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4504249.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(2766..2984)
                     /locus_tag="ACHK0Z_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             complement(2766..2984)
                     /locus_tag="ACHK0Z_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4504250.1"
                     /translation="MTTLFRKEYPRKSRATEFLFLIIFIVLMIPIPPLLLVWFIVKII
                     EPVIELYNDVVWASFSTLHNKINPYKES"
     gene            complement(3210..3707)
                     /locus_tag="ACHK0Z_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /pseudo
     CDS             complement(3210..3707)
                     /locus_tag="ACHK0Z_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(3704..3880)
                     /locus_tag="ACHK0Z_RS21710"
                     /old_locus_tag="pgaptmp_004342"
     CDS             complement(3704..3880)
                     /locus_tag="ACHK0Z_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504251.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            complement(3873..4055)
                     /locus_tag="ACHK0Z_RS21715"
                     /old_locus_tag="pgaptmp_004343"
     CDS             complement(3873..4055)
                     /locus_tag="ACHK0Z_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504252.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(4089..4301)
                     /locus_tag="ACHK0Z_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             complement(4089..4301)
                     /locus_tag="ACHK0Z_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504253.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GNAPNYCWWEVDPVDVANEAMSYHND"
     gene            complement(4352..4708)
                     /locus_tag="ACHK0Z_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             complement(4352..4708)
                     /locus_tag="ACHK0Z_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504254.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(4686..5147)
                     /locus_tag="ACHK0Z_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             complement(4686..5147)
                     /locus_tag="ACHK0Z_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HGU4504255.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            complement(5144..5440)
                     /locus_tag="ACHK0Z_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             complement(5144..5440)
                     /locus_tag="ACHK0Z_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4504256.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAR"
     gene            complement(5437..5829)
                     /locus_tag="ACHK0Z_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             complement(5437..5829)
                     /locus_tag="ACHK0Z_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4504257.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            complement(5845..6570)
                     /locus_tag="ACHK0Z_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             complement(5845..6570)
                     /locus_tag="ACHK0Z_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4504258.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(6604..7065)
                     /locus_tag="ACHK0Z_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             complement(6604..7065)
                     /locus_tag="ACHK0Z_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4504259.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(7058..8068)
                     /locus_tag="ACHK0Z_RS21755"
                     /old_locus_tag="pgaptmp_004351"
     CDS             complement(7058..8068)
                     /locus_tag="ACHK0Z_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HGU4504260.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            complement(8155..8592)
                     /locus_tag="ACHK0Z_RS21760"
                     /old_locus_tag="pgaptmp_004352"
     CDS             complement(8155..8592)
                     /locus_tag="ACHK0Z_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4504261.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            complement(8589..>8860)
                     /locus_tag="ACHK0Z_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             complement(8589..>8860)
                     /locus_tag="ACHK0Z_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4504262.1"
                     /translation="EGNMQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEE
                     VIPACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
BASE COUNT         2001 a         2478 c         1908 g         2473 t
ORIGIN      
        1 ccctgaccat gaccaatcag gtgatgcgga tcgtcagcag gcttaccaca acacgcacac
       61 ggctgtgtct tcacccagtg cgtgtatttc tcgttaaccc agcggcgacg tttaggtcgt
      121 ttcatgaaag attccggaga ctcaggatca acggcaatgc tgaccaccgt cttttcctgt
      181 ggcggttttt gctggtgagc gtgaggcagc ggcgcaagat tttttgtgcg ctgcttcagt
      241 atgctggtgg cggtctgctc tcccggtacg atgtcgcttt cacggtacat tgagcggatt
      301 ttttccgcac gcaaccccag cgaacgacgt aataccgctt ccggtagcgc gtccgccacc
      361 tgattgcgga ccgcccacca ggataattca gccagagata attcccgttc ctgtgcacca
      421 cttattgcgt gacgtatgac gtcaatcatc caggctgtca gattttgttg agcaagctgc
      481 ccgagtgatt cggatgtctg gtcgcgcagc tggttgtcgc agtgccagca caacaccatt
      541 gcgccggtac cataacggtg aatgacggtt tcgctgtgat gataatcgcc gtgtggccac
      601 tggcaggatt taatatggcg caacagccag tcagacaatg caccagcacc accagcagca
      661 cgaatcaccc gtgcgttact gaaaaacggc agcaatgttt tgtcttccac tagcggctgg
      721 cgaacggcag gaacaacccc ggacggcaga ttacgcatgc ttttcggttc cggctccacc
      781 agtacccggg tattgtggaa taccggcatg gattcacgac ccggcttaac gatcaccagc
      841 ccgagttccg gtaccagaac aggtcgaagt aatacccgca cgttacctcc agatgcgttg
      901 ctggaatgtg cgggacggac gcggcgggcg ttcggaataa aggagcctga cgtagattat
      961 ccagtgacgg tagtcgaggc taagggcttt cttaaactca tacccacgtc tgcggtagtt
     1021 atgaatcagc cattcggcct gttcttcagt gcagggatcg tgctgatacc agtcatattt
     1081 gaatgtgtga gaacaccgcc cttgcctgct ggcaggggcg atatcagaat tgtgatgttt
     1141 ggtattgtgc gccatcggtt ttctctgctg gcgcagcagg tgccagttgt tcaagctggc
     1201 gtgcggcaat attgtctctg atttctgttg tcgtcaacag gcaacgtgct atcatcgaat
     1261 ggtgttctat cctactccgt gaggtttacc atgcgtacaa cccaacaatt cagcattaca
     1321 ttaactaacg aaatggctga catggtgcgc gcccgtgtgg cttccggtgc ctatgcttca
     1381 gaaagcgagg tcattcgtga agggcttcgc gcactgaatg agcgcgataa agcaatcgaa
     1441 gcgtggttaa cgcattcagc cgccccctct cttgattcta tccgcgaaaa tccaaacaac
     1501 ggacgctcca tttcacaggt tcgcgccgcg attcgatccg ggaagtaatc tgcatgacat
     1561 atgaagtcat cattactcct gaggccgaac aacaaataat caacctgcac aggtatataa
     1621 cggagaaagc agggaacgtc attgctgaca attatgccaa tgcgcttctt gattatcttg
     1681 atgggttttc tacattcccg catcggggca ataaacgcga tgatattcgc caggggatgc
     1741 gggtaactca tttccgccac agaacgatta ttgcttttgc cgttgatagc agaaaagtct
     1801 ttattgtcgg tatctatcat ggtgggcaaa gttatgaaac cgatttctta taaactttta
     1861 cccacatcat tccggtgtta gaattaaccg tccgccccct ctcttactgg cggattcgta
     1921 ggctatataa atcaaagatc ccggctcatg tttatgtcgg gatctttttt cggcgattta
     1981 tccccagcgg caaatcgaat acaccaccag cgccaccgcc atcgcaattc ctaccgttgt
     2041 gaatgcttca ggccaggtca tcgtaaaata tcctccacgc ttatcagtcc gttccgctcc
     2101 agataactca tcgccttatc cggtaatttg cagtctggct tcgctttcct cagttgccag
     2161 gttaactgct ttaccagcat ggttaactca tcgaccagac gctgatatcc cactggtttg
     2221 tattcatgca atttaccggc tggctctgct gccagcgata ccagtgcgat ttccagaaca
     2281 gcaatatcca tcttatatgt gcggatgatg tcatggtcga ttgtgcccgg tatgcacagt
     2341 ctctgtgttt caatagtctc ctctgcgtga gctattaact gctctctggt aaaagtcgtc
     2401 atgccgtagc cccttcttga tatttttcaa accagaacac aactgggtca gatttcattt
     2461 caaccaatcc catacgaacc agcgctttgc ctttcccgga cgcaaggaat tcacgacgac
     2521 catcactgat aattcgccga taatcttcca gactactgca atgcttgtgc agattgcatg
     2581 ggtggcatgc cggaaccatg ttggatatat cgtcacgttc ctggtgaagc atatttccat
     2641 caaaacgaat gaccggtttt acatggtctg catgccactt ttcgccaagt tcgcagccgc
     2701 aataagcaca gcgaccgccg aacttcatgc gcagctctgc acgttgtttt ttcgtcagtg
     2761 ccatatcagc tttccttata cggattaatt ttattgtgca gtgtgctgaa cgacgcccac
     2821 accacgtcgt tatacaattc aataactggc tcaattattt tcacgataaa ccagaccagt
     2881 aatagcgggg gtatcggtat catcaacacg ataaatataa tgagaaacaa aaattctgtc
     2941 gctctacttt ttctcggata ttcttttctg aataatgtag tcatttctta ccgccctttc
     3001 aggcggcctc ctgatgtttt gagggtgcag aaatccctcc ggttaaggat taaattttta
     3061 actaaattta attattcagg cgcgataagc tgttccgcac aatccagcaa tgcttctgtc
     3121 gcttccttaa gcgttactgt atcgctatca tccagtcctg caactttttc gtacctgaca
     3181 aacgccgcgc aaagctcgtt aaacaacccc gcccgtactt cagccaggaa agcgtcggtg
     3241 gccggagttt caggaatccg tctcctcatc cgttctattg catgattgaa cccgaaatct
     3301 tcagcaagag atacgtcatc catattgtca ttgtcgtcct caatatcccg tggttctgga
     3361 attgcagact ttattaccgc attctccgcc gccagcgcca caagattagc tttcagctct
     3421 gcaattcgtt tgttttgggc ttcccgttca tccagtagtg ccagcgcaac atttggatta
     3481 aaagcagcaa taaattcagc gtttgcataa gcctgaacat ctgtttcaac caggcagtta
     3541 acatgacatt ctgcaattac gccaccgggc tctcctttcc atttttgaca aacaaaaact
     3601 cctgttatat tcccatgctg attgcccgat gtatgcccta cgatgtagca tcctttagtt
     3661 gctttctctg ctgcttcacg cagcgcctga tagtcaatct cgctcattca tcgccccact
     3721 catcacaata tgcttcgacc ggattttttc ctgcttcata atcatcacgc catgcttcag
     3781 catcagcagc actgccacca cgtaactctg catattccat taacagttca tgccattctt
     3841 caaaactgac gctgtattta gttgaaccaa aatcagccat tttgttcttc ctcttcgtct
     3901 tttatttcgt gatatgaata attgcagtag ttaaagaaaa tatcttttgc ttcgtcatgt
     3961 atttcatcag gcgtcgcatc atcatccact tcgaattcat cctcgaaatc tccaccggct
     4021 attcccgttt caataattat tttaaacttt cgcatttaac taccgccctt tcgggcggcc
     4081 tcctgacatt aatcgttgtg gtaactcatg gcttcatttg caacatcaac cggatcaacc
     4141 tcccaccagc aataatttgg tgcgtttcct tcaggtgtcc acggttctaa ttcatttttt
     4201 gccgcattct catcgccagt aattttaaaa atctgctcag agaattttct tgcccactcg
     4261 ttatattttt ccgcattaat ggctttctgt gtatttaaca taaatatacc tccagttcag
     4321 gattagattt tatttacagc gctaaattta tttattcagt tctggatttt gtcgccctgc
     4381 gtatccgcgc tttcgcgtta cgttcaatct gaattaactt ttctatattt ttccgccttt
     4441 cctgttcctc ctggcgcaat agccttacat catctgccag tctggtttct cttttcgcca
     4501 cagagagcat ccagtcaaac ggctccacaa ccgcaccgca gattttacag cggacctggc
     4561 gctctttttc gtcaacccga acagaggcgt gatgacaata tggtctttcc gatggctcat
     4621 aaagaaaatt aacctgatta cgagggtcat cctcttttac cggaaataaa acgatattgc
     4681 ttaactcatc ctctggtttt atttccatgc tcctctcctt tgatgcgaat gccagcgacg
     4741 cgtaatgcgt gttctaggtc aatcaggtaa agccaactgc cattttcttt aggtatcatg
     4801 acatgtcgct catctgcatt tatcgggtgt ccatatcgaa ggtcgtagcg agtcggtaat
     4861 tgaacttccc gcgcatccag ttcagcaata cgcttgctcc catcagagat aacgccttcg
     4921 taatactcac gctgctcgtt gagttgtgat tttgcttctt ccagtccatc cagcaaacca
     4981 gcgataatat ccgcttcccg atgacggata tgacgcttaa acgcagcaag agccgcatca
     5041 caatcccgtt cagcatttgg gctgtccggg gtagcctgat accacgccag cgtcgactga
     5101 tagttttgtg ctgcctcacg aagcgcctca tagttaacct ctctcatcga gccacctcct
     5161 gataaatcac cgcatgcccc agtttctccg ccagtgccag ctctgcctta gcgcccgctg
     5221 accgctgcca gccattcagc atgtaaatcg catccacaca acgaatcatt gccatgcaaa
     5281 tatccatgta gtgcggctgt gtcagcccgt ccggaagtac tgccggagtt aagacggtat
     5341 gcccttcccg tttcagttcc tcttccgcct tgtgaaacgc ctcacggttg aaattttcat
     5401 atcccgtcat tggaccggca atataaactc tcaccctcac tccatcacct cctgaaagtt
     5461 tccccgatag aacgccagca cacgctgcat aacttcgctc tggcggcact cacgacaaat
     5521 tatgttctgc cgtctgttgt aacgacgtat ttctccgtca ggtaactttc gaatcagtgt
     5581 cgggtcagca gccttctccg gtgtcttacg ccatacgcga tacgcctgct ctgatggaaa
     5641 taccccgcaa ccagagagcc agacatcacc actggccgca agcgcaccag ataaacgacg
     5701 aatagcggtc ttactgacac ccgttttatc tgccagttgt cgaaaagttt ctcgtccgct
     5761 caggcgcacg aattccacaa tgcgcgcctt cacttcttcc cgctcttctg gtgtaaattc
     5821 ttttgccata agcgcctccg gcaatcactt ttccgataca acacggcggg aagaatcagt
     5881 aatctgtcga acaatatccc ggtgcttgtt cagctcccgc agcgcggcgc agactcgctc
     5941 ccacttctga acctgacctt ttgcccggcg cagctcgcgg ttagccacat gcagcgatgg
     6001 taaaatcaga ccatccggat gttttctggt gaacgacggc tgtgactgca ctgtgaccgc
     6061 cacactttcc gtttttattt cttcctgtgt ttctgcttcc cgaactggta acgcaacacc
     6121 tgctggctga ggaaaggctt taccatcggt ttccgctacg gatgcagctt ccggctctgc
     6181 cggtaaatca gcgcccggta tgcagtaacg aaatttaccg ccctgattca cgcgaatcag
     6241 acgccctttg ctgattacca tggccagcga tgaattcgcc cggcgggagg taatcccgaa
     6301 catcagtgcc agctcatccg ccgtttgtgg gccatgttgt tcaatcgcct cagtcagcat
     6361 ttgcgctgtc actttcggta ccggtgacac cggttcactt tcaccagcct gaatcagcca
     6421 ccacatcgac cccttgttat ccgcttcacc gcggcgcttc agtttccaca gttcgttgac
     6481 cgcatcttca cggctgattc caaggcgggc cgccactacc tgtgaagagg ctcttttcag
     6541 tgctttcagt gcgtcaaata cggtttccat taaaatttcc tccgacaaaa tcgtttctca
     6601 gattcaaata aaaccagctg ccttccggcg ttcgtattcc tgtttcagcc gctcaattgg
     6661 cgttggccct tgcgggtgtt tcgccccttc cagttgtcgt cgcactggcg gaacactcat
     6721 cccgttacca acatgctttg cccatttcgt cagttgccgt tctgcaagta gttttaactc
     6781 accctgcgtc atctggcgct caatccctct ggtacgcatt tcgaggcaga tgtggtacag
     6841 cacaggctgt ggccacgggt atttatcact cccgtcgtat cgccaggatt cattgcgcca
     6901 gcgccggtac tcttccatca cggcatccac cgtaagacca aatggatttg ccccactctc
     6961 cgaaatcagc gcaacaaact cagccaggtc cgggggccac gtttcacccg cccggcagcg
     7021 gtccatgcac tgacggcaga ccagacggat ttgctgttca gtcatcgcac caatctgggc
     7081 aatccagagc ttcgaaggtg cggccccgtt cttctgagtc cagcggttcg aatacacctc
     7141 ccccataagc tcccacagct tccaggccgt ttccgttgct gataaatccg ttttcacgtt
     7201 cccactgttc gcgtgctgcc cggatttcct gaactgcccg tgatgccgtg ccacctgatg
     7261 ctgcatggct tacccccttg ctgactggtt ttacctgtgc cctgacgtgc tgcacgtggc
     7321 gggcaaattt ctgctcccac tgaacctgcg tgaaaacctt cccctccgcc atccagtaat
     7381 cccggaatgc ggcaagctca gcaggtgtaa attccggctc aggcagagcc ataccccaca
     7441 ctgctgcccg ttgtcgaaaa tccggcgacg gctgccagac agtagtcatc gaaaatttcc
     7501 cgatcggttc gctcaggccg tccaggtatt caggttcggc tgtctgcaac ggcgcaccat
     7561 gcgactcact ggtcggaata ctctcgcgtt cacgcacgtt atgtgtgggg tttaattctt
     7621 ttagatctgt atctttatta gttgcttttg tgtttgcgtc atgttcaaac acaacaccaa
     7681 catttgtttg aacacctgtt aaatctctct cttgttttgt ttgaacatat gcttcctttc
     7741 tgcttcttct ggcctgaaca gatgcttttc cggcggctga ttttttggtc aatttttccc
     7801 tgactgatgc cagatcttcc tcaatccgaa gatgcaccca ttcatcgctg ttatcgcaaa
     7861 aaaactcccg caaggatggt tccacatcag cccatcgctc gttagtcaga cgggaaattt
     7921 ttgccagcct gtttttaggt attggtttcc ctgtttgcca gtaattgaac atcagcaaca
     7981 aatacgcacc gtgctcctct gcggacaaat gcatggtgtc agccaggtaa tcagctatgt
     8041 acagttgcat gtatggtaat gcggccataa ttgcctcatc ttgtgacgaa ccatcctctg
     8101 gtgatattct gtgattcccc aatcaacaga atcagcaggg gtctggcata aatatcaatg
     8161 caccacaaca gactcgccgg atgacccgcc gtcgctgaaa tacgctttcc ggtaaactgc
     8221 ctggactgca tcatcatgcg catcaattgc cgtgctcaac gcttcctggg ccgccagtaa
     8281 tgcacggcgc tcaacagtgt caaaaatact gaggtgataa cgcagttcac gcggaagaac
     8341 agtcaggata gctggaatta gtgcctgaat tttttcaaca gcatcaggcg tatccttttc
     8401 aagccagcga aaaatatttt gtatgttcaa tccaataccc tctgtcgtag aggtgtcatg
     8461 cattggtgga taagtcattt caagctcaaa atatgctgtc gctatatcat cagcgatttt
     8521 tttgcgccct accctcggat aaagcagcca cgcattcatc gccatgcgga tgtgctcatg
     8581 cttgattttc atgaatcaac tcccgccgct gcttgtgcgt tagcctgata ctcaacaggt
     8641 aaaccatcgg ttggattagg gtatttatca gggcgcaatt catgcggagt tactccccag
     8701 tcaagagcct cacatgctgg tataacctcc tccgctggca ctcgtttttt aaaccaccca
     8761 cttatggttt gcggtgtttt accaagacga cgccctaatt ctgattggct cattattgac
     8821 aggattttca cttgagtact tttttgcatg tttccctccg
//
LOCUS       DAZFWP020000186         8737 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFWP020000186 DAZFWP020000000
VERSION     DAZFWP020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.186"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..331)
                     /locus_tag="ACHK0Z_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             complement(<1..331)
                     /locus_tag="ACHK0Z_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504263.1"
                     /translation="MHMIQYLIDHTEHLEKIAGYIFSCLIVMGVWIRKIIFNYKYKEP
                     KYVKKLILYLVKYERYLDKEDKSAIEKRINDKIIRDALKLQSSYNRSDVIYICNRLEK
                     RQYINQVI"
     gene            378..2441
                     /locus_tag="ACHK0Z_RS21775"
                     /old_locus_tag="pgaptmp_004355"
     CDS             378..2441
                     /locus_tag="ACHK0Z_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HGU4504264.1"
                     /translation="MSNILWGDLETYCEIPITNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIAAPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGALGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDVELARAAIDAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDGLANAALPNIPRDVIREAKSWYDESVKRKSTYGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELECTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEQGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITESPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            2447..2662
                     /locus_tag="ACHK0Z_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             2447..2662
                     /locus_tag="ACHK0Z_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4504265.1"
                     /translation="MSFKYRDNPLYYRAAREALRLEQSGEYDRAAKVWAKANRESHHE
                     LNQEWSERRSDFCLMQNMREKRKAVEE"
     gene            2659..2958
                     /locus_tag="ACHK0Z_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             2659..2958
                     /locus_tag="ACHK0Z_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4504266.1"
                     /translation="MIVYVAGPMSGYEQFNRPAFHSAAKRLTDKGYVVLNPATLPDGL
                     TQAQYMDICLAMLRCADTIYMLKEWEYSAGARAENALAEKLVLNVLFEEWDSDGL"
     gene            2948..3241
                     /locus_tag="ACHK0Z_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             2948..3241
                     /locus_tag="ACHK0Z_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGU4504267.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFISPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRAGQLREHGRLRALGFTVVVLDSKNLEGILCGEPITTK"
     gene            3214..4227
                     /locus_tag="ACHK0Z_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             3214..4227
                     /locus_tag="ACHK0Z_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGU4504268.1"
                     /translation="MRGAYYNEIDPNAAQWLRNLIAAGHIAPGEVDERSIEDVAPDDL
                     RGFTQCHFFAGIGVWSYALRLAGWPDNKPVWTGSCPCQPFSSAGKGAGFDDERHLWPA
                     LFHLISERRPELVFGEQVAGVNAWFDLVQTDVEAVDYAFGLVPFPAAGVGAPHIRDRA
                     YWVADASGERPQRWRKRLNSIEEDVWRSSTVRQHATQLPAGFCSPGGVVHPNLQHKPT
                     AGDKTRSRNLSGAIQISGMADSDDGRQQQSSWRGRSGQSYQTGNDPRRPNTINSCWDD
                     ADWLLCRDGRWRPVESGTFPLAHGITSRVGRLRAYGNAIVAPAAATFIRSFMECTGYD
                     LIC"
     gene            complement(4230..5159)
                     /locus_tag="ACHK0Z_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             complement(4230..5159)
                     /locus_tag="ACHK0Z_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504269.1"
                     /translation="MSEFGGQQPAPQQVLDLGVSTEGVFNEIEMGVLQNGISYLTQNG
                     LARICGVHRSNIADISREWEESFRHGVFARGRMEFISSYLQREQYNEPTLYIPINKDG
                     SVHYAYPEVVCMAILEFYAFVSQSAATPTALQFFRELSRAGMRGYIYTALGYIPDDPW
                     RHYHSRVSIMHGANSVPAGHFIVFNEIAGLMVDLIHAGLAVNQYTVPDISVGQHWARY
                     WSSSNYDATIGPRQRCQHYYPEEFNQSASNPQMVYAYPDNALAVFRHWLRAVYLPTKF
                     PKYLLSKAGLLAGGQTAALQIIDNFNNRALPNP"
     gene            5270..6664
                     /locus_tag="ACHK0Z_RS21805"
                     /old_locus_tag="pgaptmp_004361"
     CDS             5270..6664
                     /locus_tag="ACHK0Z_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HGU4504270.1"
                     /translation="MPQNFIPRPYQDLIINHEIDIMRCNIWAGMGMGKTVATLTTLED
                     LFMAGAETRPALVLAPLRVAASTWPDEAVKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTQLKSFRLRGGGKRAAALGKVAHKYVR
                     RWINLTGTPAPNGLVDLWGQAWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQGEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(6858..8255)
                     /locus_tag="ACHK0Z_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             complement(6858..8255)
                     /locus_tag="ACHK0Z_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4504271.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADCGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            8470..8730
                     /gene="yfhL"
                     /locus_tag="ACHK0Z_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             8470..8730
                     /gene="yfhL"
                     /locus_tag="ACHK0Z_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU4504272.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT         2255 a         2211 c         2241 g         2030 t
ORIGIN      
        1 taatcacctg atttatatac tgtctttttt ctaatctatt gcaaatgtaa ataacatcac
       61 ttcggttata agaagattgc aatttcagcg catcacgaat aattttatca tttattcttt
      121 tttctatggc actcttatcc tccttatcca gataacgttc atacttaact aaatataaaa
      181 ttaacttctt gacatattta ggctctttat acttataatt aaaaatgatc tttcttatcc
      241 agactcccat aacaatcaaa catgaaaaaa tatatccagc tattttctcc agatgctctg
      301 tatggtcaat caagtattgg atcatatgca taattaatct tttaatcagt taatagaata
      361 aaaaccaagg taaacacatg tccaatatac tatggggcga cctggaaacc tattgcgaaa
      421 tccctattac gaacggtacc catgcgtatg ccgaaggcgt tgaagtgatg ctgttcgcat
      481 gggctatcaa cgacgggcca gtaaacgtat gggatatcac tgccggtggt ggtattccac
      541 acggcttata cgaagcaatc gcagcccctg aaaccctgct ttattttcat aactctcact
      601 tcgaccgcac cgtcctgcgt tatgcaatgc cgcgactggc accgccagtc gaacgttggc
      661 gcgacacgat ggtgcaggcg ctggcgcacg gtctcccggg ggctctgggg gaactctgcg
      721 aagtactagg cgtcccgcaa gacaaagcga aggacaaaga aggtaaagcg ctgatccagc
      781 tgttctgtaa gccacgcccg aaaaacagca aactgcgccg ggccaccagc aaaacccacc
      841 cggaagaatg gcggcgcttt gttgcttacg ccggactgga tatcgaggca atgcgcgaag
      901 tctataaacg tctgccgaag tggaattatc aggggacaga actggcgctc tggcatcgtg
      961 atcagcagat caatgaccgg ggcgtctgca tggacgtgga actcgcacgc gctgcgatcg
     1021 acgcggtaga ccaggaacaa aagcgcctgg caaagcgtac acaggaaatg actgatggcg
     1081 aagtgcaggc agccacacaa cgagacgcgt tgattaagca cattgttgaa tcctacggtg
     1141 tggagctacc agacatgcaa cgcagtactc tggaacgtcg tattgccgac cccgatttac
     1201 catctgccgt gaaagaactg ctggctatcc gcctgcaggc cagtactacc agcaccagta
     1261 aatacaaggc actgatgaaa ggcgtaagcc acgacgggcg cttacgcggt acgctacagt
     1321 tctgcggggc gtcacgtacc ggtcgttggg ccggacggct attccagccc cagaaccttc
     1381 cccgcccttc actaaaacag gaacaaatag acgaaggcat cgaagcactg aaagccggat
     1441 gtgcagacct gctgtttgac aatatcatgg aactaaccag ttcagcgtta cgtggctgca
     1501 ttatcgcgcc aacaggcaaa aagctggtgg taagtgactt gtcgaacatt gaaggccgta
     1561 tgctggcatg gctggcggga gaagaatgga aactgaatgc attcagagag tacgacgccg
     1621 gaacgggtcc ggacttatat aaactggcgt atgcaaaagc tttcgatatt gcaccagatg
     1681 atgttgataa acacatgcgt cagatcggta aagtcatgga actcggtctg ggttatggag
     1741 gaggtgtatc ggctttcatc acttttgctc tggtttacgg tctcgatctc gacgggctgg
     1801 cgaacgccgc actgccaaac attccccgcg atgttatccg cgaggcgaaa agctggtacg
     1861 acgaatcggt taaacgtaaa tcgacctacg ggctttctga acgggtattt atcgcctgcg
     1921 actcacttaa acgtctctgg cgccgggcgc atcccgcgac ctgtgatttc tggtacgaac
     1981 tggagtgcac cgtccgcaca gcaatcgcca caccgcaaaa aacattgtat tgcggttatc
     2041 ttaaaatccg ccgcgatggc gcgtggctgc gcatacagct accatccgga cgcgctgtat
     2101 gctacccgtc tccggttatc gaacaaggga atatcaccta catgggtgtt aactcttatt
     2161 cgcgtaaatg gcaacgactc aaaacctacg gcggaaagct ggtggagaac gtcacccagg
     2221 cggccgcccg cgacgttctg gccggaaaca tgccgcttat cgaagatgcc ggttacagca
     2281 ttgtgctgac ggtacacgac gaggtgatca ccgaatcacc tgacacagaa gatttcaacg
     2341 ataaagcgct ttccgcgctt ctctccacta accccgaatg ggcgccagat atcccactga
     2401 acgctggcgg ttttgaggcg taccactacc gtaaggatta actcctatgt catttaaata
     2461 ccgggacaat ccactttatt acagggctgc aagggaggct ttgcgacttg aacaatccgg
     2521 cgaatatgac cgggcagcga aggtctgggc aaaagccaac cgcgaatcac atcacgaact
     2581 gaatcaggaa tggagtgaac gcagatctga tttttgcctg atgcagaata tgcgtgaaaa
     2641 gcgtaaggcg gtcgaagaat gatcgtttac gttgccgggc cgatgagcgg ttacgagcaa
     2701 tttaaccgcc cggcctttca ttccgcagca aagaggctaa cggacaaggg atatgttgtg
     2761 cttaaccccg caactttacc cgatggtttg acacaggctc agtacatgga catctgcctc
     2821 gcaatgcttc gctgtgccga tacgatttat atgctcaaag agtgggagta ctctgccggc
     2881 gcacgtgcag aaaacgcgtt ggcagagaaa ctggtattga atgtgctttt tgaagagtgg
     2941 gattcagatg gcctatgaac gtgaaagcct tatcgaaaag cacctcgtcg ctgaagtgaa
     3001 aaaagctggc ggagtggcct ttaagtttat atctcccggt cgccgctcgg taccagatcg
     3061 cattgttctg ctacccggtg gtcgtatcgt tttcgttgaa tgtaaagcac ccggcaagcc
     3121 accacgcgcc ggccaactgc gtgaacacgg acgactgcgc gcgctgggct ttaccgtagt
     3181 ggtgctggat agcaaaaatc tggaggggat attgtgcggg gagcctatta caacgaaata
     3241 gaccctaacg cagcacaatg gctgcgtaac cttattgctg cgggtcatat cgccccgggt
     3301 gaagttgatg aaaggagtat cgaagatgtc gcaccagacg acttgcgcgg attcacacag
     3361 tgccatttct tcgccgggat cggcgtctgg tcttatgctc tgcgtctggc cggatggccg
     3421 gataacaaac cggtctggac aggaagttgc ccgtgccagc ctttcagctc ggctggtaaa
     3481 ggcgctggat ttgatgacga gcggcaccta tggccagcgc tattccacct catcagcgag
     3541 cgacgccctg agctggtatt tggcgaacag gttgcgggag tcaatgcctg gttcgatctt
     3601 gtacaaactg acgtggaagc agtggactac gcctttgggc ttgtcccgtt tccggctgcg
     3661 ggcgtcggtg ccccgcatat ccgagataga gcgtattggg tggccgatgc cagcggcgag
     3721 cgaccacagc ggtggcgaaa gcgcctcaat agcattgagg aagatgtctg gagatcgtcg
     3781 accgtccggc aacacgctac acagctccct gcgggatttt gctcacctgg cggggtggta
     3841 caccccaacc tgcaacacaa acccacagcc ggagacaaaa cgcggtctag aaaccttagc
     3901 ggggctatcc agattagcgg gatggccgac agtgacgacg gtcgacaaca acaaagttcg
     3961 tggcgagggc gcagcggcca atcataccaa acggggaacg accctcggcg gcccaatacg
     4021 attaacagct gctgggatga tgctgactgg ctcctctgcc gggatggtcg ctggcggcca
     4081 gttgaatccg gcacattccc gttggctcat gggattacct ccagagtggg acgactgcgc
     4141 gcctacggca acgcgatagt cgcaccagct gccgcaacat tcatccgctc gtttatggaa
     4201 tgcacaggat acgacctcat ctgttgatat tatgggttag gtaatgcacg attattaaaa
     4261 ttatcaataa tttgcaacgc cgccgtttgt ccgccagcca atagccctgc tttactcaga
     4321 aggtatttag ggaatttggt tggtaaataa accgcacgaa gccagtgcct gaatacagct
     4381 aaagcattat cagggtatgc atagaccatt tggggattac ttgcggattg gttgaattct
     4441 tcagggtaat agtgctggca gcgttgtctt gggccaatcg tcgcatcgta gttggaagaa
     4501 gaccagtagc gagcccagtg ctggcccaca ctgatatccg gtacagtgta ctggtttact
     4561 gctaatccag catggatgag gtctaccatt aaaccagcaa tttcattgaa tacaatgaag
     4621 tgtccagcag gaaccgagtt tgccccatgc atgattgaca cacgagagtg gtaatgacgc
     4681 caagggtcat caggtatgta gccaagcgct gtatagatgt aaccacgcat acccgcacgg
     4741 gatagctcgc ggaaaaactg tagcgcagta ggagttgcag cactttgaga tacaaatgcg
     4801 tagaactcca gaatagccat acagactact tctgggtacg cataatggac tgacccatct
     4861 ttgttgattg ggatataaag agtcggctca ttatattgct cacgctgtaa atatgagctg
     4921 ataaactcca tacgccctcg agcgaaaact ccgtggcgga agctctcttc ccactctcga
     4981 gaaatatccg cgatattact gcgatgcacc ccgcaaatcc tagccagccc attttgcgta
     5041 aggtaggaga tcccgttctg aagaaccccc atttctattt cgttaaaaac cccctcagta
     5101 ctaacaccta agtccagtac ttgttggggt gcgggttgct gaccaccaaa ttcactcatt
     5161 ttattgttta tccttacatt taaccctgtg ggttaccttc gctgtgaata gtaaaaattg
     5221 ccaggccaac tggtgcgacc tctaactagc aaaaatttgc gagattataa tgcctcaaaa
     5281 ctttatccct cgcccctacc aagatctcat tatcaaccac gaaatcgaca tcatgcgctg
     5341 caacatctgg gcaggtatgg gaatgggtaa aaccgtagcg acactcacca cgctggaaga
     5401 tcttttcatg gctggagcag aaacacgccc cgcgctggtc ctcgcgccgc tacgcgttgc
     5461 agcaagcacc tggcctgatg aagctgttaa atgggggcat ctgcgtaata ttgaggtgca
     5521 gccgattgtt ggtaacgcca aagcgcgctc tgcagcgctg gcgaacagca acgcaagcgt
     5581 gttcaccatc aactacgata accttgtctg gctggttgag gaattgggag aacgatggcc
     5641 gttcggtact gtcattccag atgaaagcac ccagctaaaa tccttccggc tgcgaggtgg
     5701 tggtaagcgc gcggcggcgc tgggcaaagt ggcgcataag tatgtccggc gctggataaa
     5761 tctcaccggt acgccagcac cgaacggcct ggtagatttg tggggacaag cgtggtttgt
     5821 ggaccagggg caacgtctcg gacgcactta cggcgcgttt acctcacgct ggttcaactc
     5881 gatacagttt ccggggcaga gctggaccaa actggagccg tttgctcact cacagggtga
     5941 aatacagcga gcgttagccg atgtgaccct atcgctggat gcggccgact ggttcgatat
     6001 caaagacccc atccataacg taatccgcgt ggatatgccg ccgaaggccc gtcagcagta
     6061 tcgtgaaatg gaaaaggaaa tgttcctcga gctgaatggc gaaggcatcg aagcaccaaa
     6121 cgccgcggca aagacactga agtgtctgca aatcgccagt ggcgcagtat acacagacga
     6181 caccggaagt tggtcagaac tgcatgacac caaactgcag gcgctggaca gcatactgac
     6241 cgaagcagct ggcgcacctg tgctggttgc ttatcactgg aaacacgatc ttgaacgctt
     6301 gcttaaagca ttccctcgcg gtcgtcacct cgaccaagat ccacagacac tgcgcgactg
     6361 gaattccgga aagattcctg ttctctttgc acacccagcc agcgcgggcc acggtctgaa
     6421 catgcaggac ggcggaaaca tactggtatt tttctcacac tggtgggacc tggagcagta
     6481 tcagcaaatt attgaacgta tcggccccac caggcagata caggccggac acaatcgtcc
     6541 ggtatttatt caccacatta ttgcggccga cactatggac gaaatggtga tggagcggcg
     6601 caactcaaaa cgaacagtgc aggacatcct gctcgatgcc atgaaaaaga gaggtatagc
     6661 atgagcgaga aacccgacga tttactcacc ccagatgaag tatgccaaaa gttaggtatt
     6721 acacagaaaa cgctatgtga gtggaatatt aagcatcgtc atcgggctat cctggcacca
     6781 attcgtttca gtgcaaaagt agttcgttat gagcgccgaa atgtcgacgc ttttattcaa
     6841 aagtgtcgca gccagtatta acctcgccgc cgtagcaatg caacctgcgc aagtatgctc
     6901 cgctcgtgag cctcgaaagc ttcgcgcttt aacgcaatct cttcctgtaa aatctcatca
     6961 gaaaagtcgt aatgttctgc catcgggtca tctgacttgc tggagtggtg aaggcaaagg
     7021 aggctgattt cccttcggtc tgatcgggaa tagcctcttt ccttcatcaa ggcaataaca
     7081 ttgctcttaa ggaatttacg gcacatcgta ttaaatgcac cgtctttccc tttaacagtc
     7141 ccatcatgtt ttattccttt tacagccccg tccgggctgt atgttttcac cagcttatcc
     7201 agtgatcgtt ttgaaaatgg ctgcattgga tcacgtggct gcaaaaatac ataatccctg
     7261 ttgcactcag gaactgaatc acgccaggct ttctgctcgt cgataatccg ccgaatctca
     7321 ggcgttatcg gcaggcggaa agccttttgt gttttcatag cccctcgcat gccgataacc
     7381 ccttccggat aaacaatttc accagcctcc tcgtgaacgt agtcccagcg caggttatgg
     7441 acattaatcg gacgaacgcc agtgatgatc atgaagcgaa cagcattctt ctggtgtaca
     7501 gaggtgcagg cagcaacatt gagccagagt cgggcgattg attcaatatc ggtaaaaagc
     7561 cgtgtggggg taggtttctg tacgcgggag gaaacataat catctggcag actggcggca
     7621 acattgcggc cgttgcaaag agtaggtgcg cagaacttcc agaaccgacg gagctcggca
     7681 aacaactcca gggcgttatt gttcgagcga gtggcgatcc actcgtccag cacttccacc
     7741 agccgattgt atgttacgtc gctgaacacc tcacgctcgc cgaacgttgc tttaatccgg
     7801 tcgatacgca ccccgtaggt tgtgaaactg tccgggctca gcttctgccg ggcgactttg
     7861 gctttgaggt catcccggta catttccagc gctgcatgta ctgactctgc ccgcaagcca
     7921 cagtcagcca tacctagcgc tttttcgcgc gccagctgga tagcgagctc cggccactcg
     7981 ccgagctttt tacccttgag gcccatcttt tttggaaact cggcgtaaaa tgtaacctta
     8041 ccggctttgc tgaaatcgat acggagatag ttctcttttt cgtatttgga acggcgagcc
     8101 acgccggaag cagcgaggat gattttggcg gcagcaacac agattttcat gtgtgcgctg
     8161 gtataggggg gtttacaggc gtcccatttt tcagatgcgg ctaaaacatc gtcattattg
     8221 gggctatccg gattatgtgt tacagtgcgc ggcattctca atccttatct gcgtaggcgc
     8281 agaaaacaag ctcacacata caagtctttt ctacgggaca aaatgcaatg tgttgcggtt
     8341 ttgtgttgct ggaatgagtt tatcaaggtt aaatacactg gatcaacata cagtaagtta
     8401 atgacagtaa agcatacaaa ctcgatacaa cttactgatt ttaaaatgat ttaacggtaa
     8461 tccattgaaa tgtctttact aattactaaa cgctgtatta attgtgatat gtgtgaaccc
     8521 gaatgcccga atgaggcgat ttcaatggga gatcatatct acgagattaa cagcgataag
     8581 tgtaccgaat gcgtagggca ctacgagaca ccaacctgcc agaaggtgtg cccgatcccc
     8641 aatactattg tgaaagatcc ggcgcatgtc gagacagaag aacagttgtg ggataaattt
     8701 gtgctgatgc accacgcgga taaaatttaa tattcta
//
LOCUS       DAZFWP020000187         8655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFWP020000187 DAZFWP020000000
VERSION     DAZFWP020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.187"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(309..572)
                     /gene="rpsT"
                     /locus_tag="ACHK0Z_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             complement(309..572)
                     /gene="rpsT"
                     /locus_tag="ACHK0Z_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU4504273.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            675..893
                     /locus_tag="ACHK0Z_RS21825"
                     /old_locus_tag="pgaptmp_004365"
     CDS             675..893
                     /locus_tag="ACHK0Z_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU4504274.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            901..1842
                     /gene="ribF"
                     /locus_tag="ACHK0Z_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             901..1842
                     /gene="ribF"
                     /locus_tag="ACHK0Z_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU4504275.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLALGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1885..4701
                     /gene="ileS"
                     /locus_tag="ACHK0Z_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             1885..4701
                     /gene="ileS"
                     /locus_tag="ACHK0Z_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU4504276.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4701..5195
                     /gene="lspA"
                     /locus_tag="ACHK0Z_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             4701..5195
                     /gene="lspA"
                     /locus_tag="ACHK0Z_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU4504277.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5283..5732
                     /gene="fkpB"
                     /locus_tag="ACHK0Z_RS21845"
                     /old_locus_tag="pgaptmp_004369"
     CDS             5283..5732
                     /gene="fkpB"
                     /locus_tag="ACHK0Z_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4504278.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5734..6684
                     /gene="ispH"
                     /locus_tag="ACHK0Z_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /gene_synonym="lytB"
     CDS             5734..6684
                     /gene="ispH"
                     /locus_tag="ACHK0Z_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU4504279.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6750..7664
                     /gene="rihC"
                     /locus_tag="ACHK0Z_RS21855"
                     /old_locus_tag="pgaptmp_004371"
     CDS             6750..7664
                     /gene="rihC"
                     /locus_tag="ACHK0Z_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU4504280.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7831..8652
                     /gene="dapB"
                     /locus_tag="ACHK0Z_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             7831..8652
                     /gene="dapB"
                     /locus_tag="ACHK0Z_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU4504281.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESDLFDMRDVLDLNSL"
BASE COUNT         1970 a         2157 c         2468 g         2060 t
ORIGIN      
        1 tgtataggaa tttaattcaa aaatagagtg gtcttaagtt taatacctca atgtgtatca
       61 caatatccac attctttgta ggggagtctg gagattgagt agatattctt gttcagaatg
      121 tatcagccga tggttctacg attcttaagc cacgaagagt tcagaaagtg taacggcatg
      181 tctgcaaaag ccagcatctt ttgactatct ggcaaccggt agtgtgttct ctcacgcatc
      241 ataaaagcag caggcataaa aaaacccgct tgcgcgggct ttttcacaaa gcttcagcaa
      301 attggcgatt aagccagttt gttgatctgt gcagtcaggt tagccttatg acgtgcagct
      361 ttgtttttgt ggatcagacc tttagcagcc tgacggtcca cgatcggttg catttcgtta
      421 aatgctttct gtgcagcagc tttgtcgcca gcttcgatag ctgcgtatac tttcttgatg
      481 aaagtacgca tcatagagcg acggcttgcg ttgtgcttac gagccttttc agactgaatg
      541 gcgcgcttct tagctgattt gatattagcc aaggtccaac tcccaaatgt gttctatatg
      601 gacaattcaa aggccgagga atatgccctt ttagccttct tttgtcaatg gatttgtgca
      661 aataagcgcc gttaatgtgc cggcactcgt tacgtagtga tggcgcagga ttctaccagc
      721 ttgcggggtg tgaatacagc ttttccgcga taaaaattgc agcaggcggt cactttcttc
      781 ccgtgatttg cgccatggca atgaaaagcc acttctttct gatttcggta ctcaatcgcc
      841 ggttaacctt gaccgctgta caaggtatac tcggacgatt ttcactgttt tgagccagac
      901 atgaagctga tacgcggcat acataatctc agccaggccc cgcaagaagg gtgtgtgctg
      961 actattggta atttcgacgg cgtgcatcgc ggtcatcgcg cgctgttaca gggcttgcag
     1021 gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc tttttgaacc tcaaccactg
     1081 gaactgtttg ctaccgataa agccccggca cggctgaccc ggctgcggga aaaactgcgt
     1141 taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc gtttcgacag gcgtttcgcg
     1201 gcgttaaccg cgcaaaattt catcagcgat cttctggtga agcatttgcg cgtaaaattt
     1261 cttgccttag gtgatgattt ccgctttggc gctggtcgtg aaggcgattt cttgttatta
     1321 cagaaagctg gcatggaata cggcttcgat atcaccagta cgcaaacttt ttgcgaaggt
     1381 ggcgtgcgta tcagcagcac cgctgtgcgt caggcgctgg cggatgacaa tctggctctg
     1441 gcagaaagtt tactggggca cccgtttgcc atctccgggc gtgtagtcca cggtgatgaa
     1501 ttagggcgca ctataggttt cccgacggcg aatgtaccgc tacgccgtca ggtttccccg
     1561 gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg aaaagccgtt acccggcgtg
     1621 gcaaacattg gaacacgccc aacggttgcc ggtattcgcc agcagctgga agtgcatttg
     1681 ttagatgttg caatggacct ttacggtcgc catatacaag tagtgctgcg taaaaaaata
     1741 cgcaatgagc agcgatttgc gtcgctggac gaactgaaag cgcagattgc gcgtgatgaa
     1801 ttaaccgccc gcgaattttt tgggctaaca aaaccggctt aagcctgtta tgtaatcaaa
     1861 ccgaaacacg gaaccgagaa tctgatgagt gactataaat caaccctgaa tttgccggaa
     1921 acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac cgggaatgct ggcgcgttgg
     1981 actgatgatg atctgtacgg catcatccgt gcggctaaaa aaggcaaaaa aaccttcatt
     2041 ctgcatgatg gccctcctta tgcgaatggc agcattcata ttggtcactc ggttaacaag
     2101 attctgaaag acattatcgt gaagtccaaa gggctttccg gttatgactc gccgtatgtg
     2161 cctggctggg actgccacgg tctgccgatc gagctgaaag tagagcaaga atacggtaag
     2221 ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt gccgcgaata cgcggcgacc
     2281 caggttgacg gtcaacgcaa agactttatc cgtctgggcg tgctgggcga ctggtcgcac
     2341 ccgtacctga ccatggactt caaaactgaa gccaacatca tccgcgcgct gggcaaaatc
     2401 atcggcaacg gtcacctgca caaaggcgcg aagccagttc actggtgcgt tgactgccgt
     2461 tctgcgctgg cggaagcgga agttgagtat tacgacaaaa cttctccgtc catcgacgtt
     2521 gcttttcagg cggtcgatca ggatgcactg aaagcaaaat ttgccgtaag caacgttaac
     2581 ggcccaatct cgctggtgat ctggaccact acgccgtgga ctctgcctgc gaaccgcgca
     2641 atctctattg cacctgattt cgactatgcg ctggtgcaga tcgacggtca ggccgtgatt
     2701 ctggcgaaag atctggttga aagcgtaatg cagcgtatcg gcgtgaccga ttacaccatt
     2761 ctcggcacgg taaaaggtgc ggagcttgag ttgctgcgct ttacccatcc gtttatgggc
     2821 ttcgacgttc cggcaatcct cggcgatcac gttaccctgg atgccggtac cggtgccgtt
     2881 cacaccgcgc ctggccacgg cccggacgac tatgtgatcg gtcagaaata cggcctggaa
     2941 accgctaacc cggttggccc ggacggcact tatctgccgg gcacttatcc gacgctggat
     3001 ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc tgctgcagga aaaaggcgcg
     3061 ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct gctggcgtca caaaacgccg
     3121 atcatcttcc gcgcgacgcc gcagtggttc gtcagcatgg atcagaaagg tctgcgtgcg
     3181 cagtcactga aagagatcaa aggcgtgcag tggatcccgg actggggcca ggcgcgtatc
     3241 gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc gtcagcgcac ctggggcgta
     3301 ccgatgtcac tgttcgtgca caaagacacg gaagagctgc atccgcgtac actcgaacta
     3361 atggaagaag tggcaaaacg cgttgaagtt gacggcatcc aggcgtggtg ggatcttgat
     3421 gcgaaagaga tcctcggcga tgaagctgat cagtacgtga aagtgccgga cacattggat
     3481 gtatggtttg actccggatc tacccactct tctgttgttg acgtgcgtcc ggaatttgcc
     3541 ggtcacgcag cggacatgta tctggaaggt tctgaccagc accgtggttg gttcatgtct
     3601 tccctaatga tctccaccgc gatgaaaggc aaagcgccgt atcgtcaggt actgacccac
     3661 ggctttaccg tggatggtca gggtcgcaag atgtctaaat ccatcggcaa taccgtttcg
     3721 ccgcaggatg tgatgaacaa actgggcgcg gatattctgc gactgtgggt ggcatcaacc
     3781 gactacaccg gtgaaatggc cgtttctgac gagatcctga aacgtgctgc cgatagctat
     3841 cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc tgaacggttt tgatccagca
     3901 aaagatatgg tgaaaccgga agagatggtg gtactggatc gctgggccgt aggttgtgcg
     3961 aaagcggcac aggaagacat cctcaaggcg tacgaagcat acgatttcca cgaagtggta
     4021 cagcgtctga tgcgcttctg ctccgttgag atgggttcct tctacctcga catcatcaaa
     4081 gaccgtcagt acaccgccaa agcggacagt gtggcgcgtc gtagctgcca gactgcgctg
     4141 tatcacatcg cagaagcgct ggtgcgctgg atggcaccaa tcctctcctt caccgctgat
     4201 gaagtgtggg gctacctgcc gggcgaacgt gaaaaatacg tcttcaccgg tgagtggtac
     4261 gaaggcctgt ttggcctggc agacagtgaa gcgatgaacg atgcgttctg ggacgagctg
     4321 ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag cgcgtgccga caagaaagtg
     4381 ggtggctcgc tggaagcggc agtaaccttg tatgcagaac cggaactgtc ggcgaaactg
     4441 accgcgctgg gcgatgaatt acgatttgtc ctgttgacct ccggcgctac cgttgcagac
     4501 tataacgacg cacctgctga tgctcagcag agcgaagtac tcaaagggct gaaagtcgcg
     4561 ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc actacaccca ggatgtcggc
     4621 aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca gcaacgtcgc cggtgacggt
     4681 gaaaaacgta agtttgcctg atgagtcaat cgatctgttc aacagggcta cgctggctgt
     4741 ggctggtggt agtcgtgctg attatcgatc tgggcagcaa atacctgatc ctccagaact
     4801 ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa tctgcattat gcgcgtaact
     4861 atggcgcggc gtttagtttc cttgccgata gcggcggctg gcagcgttgg ttctttgccg
     4921 gtattgcgat tggtattagc gtgatcctgg cagtgatgat gtatcgctcg aaggccacgc
     4981 agaagctaaa caatatcgct tatgcgctga ttattggcgg cgcgctgggc aacctgttcg
     5041 accgcctgtg gcacggcttc gttgtcgata tgatcgactt ctacgtcggc gactggcact
     5101 tcgccacctt caaccttgcc gatactgcca tttgtgtcgg tgcggcactg attgtgctgg
     5161 aaggtttttt gccttctaaa gcgaaaaaac aataataaac cctgccggat aaggcctaca
     5221 gattgctgcg aaatcgtagg ccgcatccgg caaaaatcct caaaatataa gagcaaacct
     5281 gcatgtctga atctgtacag agcaatagcg ccgtcctggt gcacttcacg ctaaaactcg
     5341 acgatggcac caccgccgag tctacccgca acaacggtaa accggcgctg ttccgcctgg
     5401 gtgatgcttc tctttctgaa gggctggagc aacacctgtt ggggctgaaa gtgggcgata
     5461 aaaccacctt ctcgttggag ccagatgcgg cgtttggcgt gccgtcaccg gacctgattc
     5521 agtacttctc ccgccgtgaa tttatggatg caggcgagcc agaaattggc gcaatcatgc
     5581 tttttaccgc aatggatggc agtgagatgc ctggcgtgat ccgcgaaatt aacggcgact
     5641 ccattaccgt tgatttcaac catccgctgg ccgggcagac cgttcatttt gatattgaag
     5701 tgctggaaat cgatccggca ctggaggcgt aacatgcaga tcctgttggc caacccgcgt
     5761 ggtttttgtg ccggggtaga ccgcgctatc agcattgttg aaaacgcgct ggccatttac
     5821 ggcgcaccga tatatgtccg tcacgaagtg gtacataacc gctatgtggt cgatagcttg
     5881 cgtgagcgtg gggctatctt tattgagcag attagcgaag taccggacgg cgcgatcctg
     5941 attttctccg cacacggtgt ttctcaggcg gtacgtaacg aagcaaaaag tcgcgatttg
     6001 acggtatttg acgcgacctg tccgctggtg accaaagtgc atatggaagt cgcccgcgcc
     6061 agtcgccgtg gcgaagaatc tattctcatc ggtcacgccg ggcacccgga agtggaaggg
     6121 acaatgggcc agtacagtaa cccggaaggg ggaatgtatc tggtcgaatc gccggacgat
     6181 gtgtggaaac tgacggtcaa aaacgaagag aagctctcct ttatgaccca gaccacgctg
     6241 tcggtggatg acacgtctga tgtgatcgac gcgctgcgta aacgcttccc gaaaattgtc
     6301 ggtccgcgca aagatgacat ctgctacgcc acgactaacc gtcaggaagc ggtacgcgcc
     6361 ctggcagaac aggcggaagt tgtgttggtg gtcggttcga aaaactcctc caactccaac
     6421 cgtctggcgg agctggccca gcgtatgggc aaacgcgcgt ttttgattga cgatgcgaaa
     6481 gacatccagg aagagtgggt gaaagaggtt aaatgcgtcg gcgtgactgc gggcgcatcg
     6541 gctccggata ttctggtgca gaatgtggtg gcacgtttgc agcagctggg cggtggtgaa
     6601 gccattccgc tggaaggccg tgaagaaaac attgttttcg aagtgccgaa agagctgcgt
     6661 gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa gttatgcgaa aatgccggtc
     6721 ttgttaccgg cattttttat ggagaaaaca tgcgtttacc tatcttcctc gatactgacc
     6781 ccggcattga cgatgccgtc gccattgccg ccgcgatttt tgcccccgaa ctcgacctgc
     6841 aactgatgac caccgtcgcg ggtaatgtct cggttgagaa aactacccgt aatgccttgc
     6901 aactgctgca tttctggaat gcggagattc cgctcgccca aggggccgct gtgccgctgg
     6961 tacgcgcacc gcgtgatgcg gcatctgtgc acggcgaatc gggaatggct ggctacgact
     7021 ttgttgagca caaccgaaag ccgctcggga taccggcgtt tctggcgatt cgggatgccc
     7081 tgatgcgtgc accagagcct gttaccctgg tggccatcgg cccgttaacc aatattgcgc
     7141 tgttactttc acaatgcccg gaatgcaagc cgtatattcg ccgtctggtg atcatgggtg
     7201 gttctgccgg acgcggcaac tgtacgccaa acgccgagtt taatatcgct gccgatccag
     7261 aagcggctgc ctgtgtcttc cgtagcggaa tcgaaatcgt catgtgcggt ctggatgtca
     7321 ccaatcaggc aatattaacc cctgactatc tcgctacttt gccggagttg aatcgcaccg
     7381 gaaaaatgct tcatgcactg tttagccact accgtagcgg cagtatgcaa agcggtctgc
     7441 gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc ggacctgttc actctcaaac
     7501 cctgttttgt ggcagtggaa actcagggcg aatttacctc aggcacgacg gtggttgata
     7561 tcgacggttg cctgggcaag ccagccaatg tacaggtggc attggatctg gatgtgaaag
     7621 gcttccagca gtgggtggct gaggtgctgg ctctggcgtc gtaacctgtc acatgttatt
     7681 ggcatgcagt cattcatcga ctcatgcctt tcactgatat ccctccctgt ttatcattaa
     7741 tttctaatta tcagcgtttt tggctggcgg cgtagcgatg cgctggttac tctgaaaacg
     7801 gtctatgcaa attaacaaaa gagaatagct atgcatgatg caaacatccg cgttgccatc
     7861 gcgggagccg gggggcgtat gggccgccag ttgattcagg cggcgctggc attagaaggc
     7921 gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt tactgggcag cgacgccggt
     7981 gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa gcagcctcga tgcggtaaaa
     8041 gatgattttg atgtgtttat cgattttacc cgtccggaag gtacgctgaa ccatctcgct
     8101 ttttgtcgcc agcatggcaa agggatggtg atcggcacta cggggtttga cgaagccggt
     8161 aaacaggcga ttcgtgacgc cgccgccgat attgcgattg tctttgccgc caattttagc
     8221 gttggcgtta acgtcatgct taagctgctg gagaaagcag ccaaagtgat gggtgactac
     8281 accgatatcg aaattattga agcacatcat agacataaag ttgatgcgcc gtcaggcacc
     8341 gcactggcaa tgggagaggc gatcgcccac gcccttgata aagatctgaa agattgcgcg
     8401 gtctacagtc gtgaaggcca caccggtgaa cgtgtgcctg gcaccattgg ttttgccacc
     8461 gtgcgtgcag gtgacatcgt tggtgaacat accgcgatgt ttgccgatat tggcgagcgt
     8521 ctggagatca ctcataaggc gtccagccgc atgacatttg ctaacggcgc ggtaagatcg
     8581 gctttgtggt tgagtggtaa ggaaagtgat ctttttgata tgcgagatgt gcttgatctc
     8641 aatagtttgt aacca
//
LOCUS       DAZFWP020000188         8594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFWP020000188 DAZFWP020000000
VERSION     DAZFWP020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.188"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            33..551
                     /gene="ydeJ"
                     /locus_tag="ACHK0Z_RS21865"
                     /old_locus_tag="pgaptmp_004373"
     CDS             33..551
                     /gene="ydeJ"
                     /locus_tag="ACHK0Z_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU4504282.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(595..2640)
                     /gene="dcp"
                     /locus_tag="ACHK0Z_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             complement(595..2640)
                     /gene="dcp"
                     /locus_tag="ACHK0Z_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU4504283.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQHRGLNI"
     gene            2777..3523
                     /gene="ydfG"
                     /locus_tag="ACHK0Z_RS21875"
                     /old_locus_tag="pgaptmp_004375"
     CDS             2777..3523
                     /gene="ydfG"
                     /locus_tag="ACHK0Z_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU4504284.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3612..4298
                     /gene="rspR"
                     /locus_tag="ACHK0Z_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /gene_synonym="ydfH"
     CDS             3612..4298
                     /gene="rspR"
                     /locus_tag="ACHK0Z_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU4504285.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4476..4679
                     /gene="ydfZ"
                     /locus_tag="ACHK0Z_RS21885"
                     /old_locus_tag="pgaptmp_004377"
     CDS             4476..4679
                     /gene="ydfZ"
                     /locus_tag="ACHK0Z_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU4504286.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4715..6175)
                     /gene="ydfI"
                     /locus_tag="ACHK0Z_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /pseudo
     CDS             complement(4715..6175)
                     /gene="ydfI"
                     /locus_tag="ACHK0Z_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(6264..7547)
                     /gene="ydfJ"
                     /locus_tag="ACHK0Z_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /pseudo
     CDS             complement(6264..7547)
                     /gene="ydfJ"
                     /locus_tag="ACHK0Z_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            7679..7921
                     /locus_tag="ACHK0Z_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             7679..7921
                     /locus_tag="ACHK0Z_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4504287.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDAEVIIKSTS
                     NNGLSVTRTADKDSAKTFVQETLKDTRESADEWFVR"
     gene            complement(8030..8187)
                     /locus_tag="ACHK0Z_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /pseudo
     CDS             complement(8030..8187)
                     /locus_tag="ACHK0Z_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
BASE COUNT         2290 a         2170 c         1939 g         2195 t
ORIGIN      
        1 acctttacat ataaaagcgt ggacaaaaaa cagtgaacat taatagagat aaaattgtac
       61 aacttgtaga taccgatact attgaaaacc tgacatccgc gttgagtcaa agacttatcg
      121 cggatcaatt acgcttaact accgccgaat catgcaccgg cggtaagttg gctagcgccc
      181 tgtgtgcagc tgaagataca cccaaatttt acggtgcagg ctttgttact ttcaccgatc
      241 aggcaaagat gaaaatcctc agcgtaagcc agcaatctct tgaacgatat tctgcggtga
      301 gtgagaaagt ggcagcagaa atggcaaccg gtgccataga gcgtgcggat gctgatgtca
      361 gtattgccat taccggctac ggcggaccgg agggcggtga agatggtacg ccagcgggta
      421 ccgtctggtt tgcgtggcat attaaaggcc agaactacac tgcggttatg cattttgctg
      481 gcgactgcga aacggtatta gctttagcgg tgaggtttgc cctcgcccag ctgctgcaat
      541 tactgctata accaggctgg cctggcgata tctcaggcca gccattggtg gtgtttatat
      601 gttcaagcca cgatgttgca gcatcggcat aatcttaggt gccttaccgc gccattgtcg
      661 atacaggcgt tccagatctt cgctgttacc tctggaaagg atcgcctcgc gaaaacgctg
      721 cccattttca cgcgttaatc cgccctgctc aacaaaccac tggtaaccat catcggccaa
      781 catttgcgtc cacagataag cgtaataacc tgcagcatat ccgccaccaa aaatatgggc
      841 gaaataactg ctgcgatagc gtggcggtat agcaggaaga tccatatttt ccgccaccag
      901 cgcccgcaat tcaaaatcat cgacatcctg cattgcttcg ttttcttcca ggcaatgcca
      961 gcgcatatcg agaagtgcgg cgctaagcag ttcgctcatc tcataccctt tgttgaacag
     1021 gctggcatta cgcattttct gttgcagttc gtcaggcatt gctgccccgc tctgataatg
     1081 ccgggcgtag cgagcgaata cctgcggatg cgttgcccag tgttcgttga tttgcgacgg
     1141 aaattcgaca aaatcacgcg gcgtgttggt gccggaaagc gtggcataac gctggcgggc
     1201 aaaaaggccg tgcagcgtat gaccaaattc atggaataag gttattacat catcccagag
     1261 taacaacgca ggctcaccgg cagcgggttt ctgataattg caaacgttat aaattaccgg
     1321 atgcgtttca ttaagcgttg attgctcaac aaaattgccc atccatgcac cgccgctttt
     1381 tgaatcacgg gcgaagaaat caccgtaaaa taacgccagt cccacgccat tatgatcaaa
     1441 aatttcccac acacgaacgt caggatggta gacaggaata tcaaaacgtt cgacaaactt
     1501 aataccgaag agctgattcg cggtccagaa tacaccttca ttcaacaccg tgtttaattc
     1561 aaaatatggc ttgagctgcg cctcatcaag atcaaatttc tcccgccgta cctgttcggc
     1621 ataaaatgcc cagtcccacg gctgcgcgct aaacccgcct tgctgcttat cgataaccgc
     1681 ctgtatggag gctaattcat cgctagcacg ttgacgcgcc gctggaacaa tttcccgcat
     1741 aaagttgagt gctgcttcag gtgtttttgc catctgatcg gcgattttcc atgcggcata
     1801 atgaggaaaa ccaagcagtt tcgcctgctg tgcgcgaatc tctaccagac gttgaatgat
     1861 agcgcgggta tcattgccat catttttttc cgctcgcgtc cagcccgcaa taaacagttt
     1921 ttcacgcgtc gcacgatcgc gcatttcggc aagcgccggt tgttgggtgg tattcagcag
     1981 cggaatcagc catttgttat ccagaccttt ctcgcgagcc gccactgccg ccagcgcaat
     2041 ctcttgctca ctcattcctg ccagctgcgc gatatcgttc acaaccagac cgccggattt
     2101 atttgctgca agtaaccgct ggttaaactg gctggtcagg gtcgcagctt ctgtattcag
     2161 tacttttaat tttgctttat cagcttgcgc aagtttggct ccggcaagga caaaacgttg
     2221 atgaataacc tccaccaggc ggatggattc actatcaagc cccagggatt cacggcgctg
     2281 ccagacagca tctacccgcg cgaataattc accgttcaga tagatatcat tagccagttc
     2341 cgccagttca gcggaaaact gttcatcaag acgctgtaat tcatcattgg tatgcgccgc
     2401 agtcatcgca aaaaagacgc tggtaacgcg ggtaagtaat tctccgcttt gttccagtgc
     2461 cagaatagta ttgttgaaat caggcgtttg cgggttaagc gcgatggcag caatttctgc
     2521 ccgcttttgc tgcattccct catcgaatgc cgggcgatag tgatgattgg caatttgatc
     2581 aaaatgagga gccagatacg gcagtgtgct ttgcacaagg aaaggattca ttgttgtcat
     2641 tttcttctcc tgaacgcgag gtgttccata gcgtaggctt actgataacg tagtgcaatc
     2701 ttgcaaacca gtattacccg ctcttaagca tcacgtgcta tgttattgac acacaaaagc
     2761 gttgaggaac agtgagatga tcgttttagt aactggagca acggcaggtt ttggtgaatg
     2821 cattactcgt cgttttattc aacaagggca taaagttatc gccactggcc gtcgccagga
     2881 gcggttgcag gagttaaaag acgaactggg agataatctg tatatcgccc aactggacgt
     2941 tcgcaaccgc gccgctattg aagagatgct ggcatcgctt cctgccgagt ggtgcaatat
     3001 tgatatcctg gtaaataatg ccggcttggc gttgggcatg gagcctgcgc ataaagccag
     3061 cgttgaagac tgggaaacga tgattgatac caacaacaaa ggcctggtat atatgacgcg
     3121 cgccgtctta ccgggtatgg ttgaacgtaa tcatggtcat attattaaca ttggctcaac
     3181 ggcaggtagc tggccgtatg ccggtggtaa cgtttacggt gcgacgaaag cgtttgttcg
     3241 tcagtttagc ctgaatctgc gtacggatct gcatggtacg gcggtgcgcg tcaccgacat
     3301 cgaaccgggt ctggtgggtg gcaccgagtt ttccaatgtc cgctttaaag gcgatgacgg
     3361 taaagcggaa aaaacctatc aaaataccgt tgcattgacg ccagaagatg tcagcgaagc
     3421 cgtctggtgg gtgtcaacgc tgcctgctca cgtcaatatc aataccctgg aaatgatgcc
     3481 ggttacccaa agctatgccg gactgaatgt ccaccgtcag taatttttat acccggcgta
     3541 actgccgggt tattgcttgt cacaaaaaag tggtagactc atgcagttaa ctcactcaca
     3601 agcaagaacg aatgaccgtc gaaacgcaac ttaatcccac acagcctgtc aatcagcaga
     3661 tttatcgtat tcttcgtcgc gacattgtcc attgcctgat tgcaccaggc acaccgttgt
     3721 cggaaaaaga agtttctgtt cgtttcaatg tgtcacgcca gccggttcgt gaagccttta
     3781 ttaaactggc ggaaaacggc ctgattcaaa ttcgtccgca gcgtggcagc tacgtcaaca
     3841 aaatttccat ggcccaggtg cgaaacggca gttttatccg tcaggccatt gagtgcgcgg
     3901 tggcgcgtcg ggcggcgagc atgattaccg aaagccagtg ctaccaactg gaacaaaatc
     3961 ttcaccagca acgcattgcc attgagcgca agcaactgga tgattttttt gaacttgatg
     4021 acaacttcca tcaactcctg acgcagattg ccgactgtca actggcgtgg gataccattg
     4081 agaacctgaa agcgaccgtt gatcgcgtgc gctatatgag tttcgaccat gtttctccac
     4141 cagaaatgct gttacgccag catcttgata ttttctctgc cctgcaaaaa cgtgatggcg
     4201 atgcggtaga acgtgcaatg acgcaacatt tgcaggaaat cagcgaatcc gtgcgccaga
     4261 tccgccagga aaacagcgac tggtttagcg aagagtaatt catttactct catcccatcc
     4321 gggatgagag gcttttcccc ccgccttacg gctcatgcat gcatcaaaaa agatgtgagc
     4381 ttgatcaaaa acaaaaaata tttcactcga caggagtatt tatattgcgc ccgttacgtg
     4441 ggcttcgact gtaaatcaga aaggagaaaa cacctatgac gacctacgat cgtaaccgta
     4501 acgcaatcac cactggcagc cgtgttatgg ttagcggcac cggtcacact ggcaagatcc
     4561 tgtcgattga tactgaaggt ctgaccgctg agcaaatccg ccgcggaaaa accgtagttg
     4621 ttgaaggttg tgaagagaaa ctggcacctc tggacctgat tcgtctcggc atgaactaag
     4681 tgtgtgaatg ccgccgatgg cggcattgct ttttttactt cacggaatat ttcgccacgg
     4741 tcgctttcgc gccatgcgct aataaagaca agtacgcttc cgtcactttt gcagtaaaca
     4801 agctatcgtc tggcaaatca tcaccaaaga ttgccttaat cgccagcaat gactggacgc
     4861 gcgctttccc ttcggcacta ctttgtacag ccttctgaat aacaggtaac agtgggtcac
     4921 tgatttctat cggatttccc tgttcatcaa caccaccgac ataacgcatc caacccgcga
     4981 cgcccagcgc cagcagatcg aacttgctgt catgcgccag atgccagcga acagaatcca
     5041 acatccgctg tggcaatttc tggctgccat ccatcgcaat ctgccaggtt cgatgacgta
     5101 acgccgggtt gctatagcgt gcaattaatc ggttagcgta atcctgcaaa tcaacgccct
     5161 gcactttcag cgtcggcgct tgttcctgca acatcaagcc atacgccgca tgacgataat
     5221 gttcatcttc catgcagtca ttaatgtgct gatatccggc aagatacccc agatacgcca
     5281 ggaatgaatg actgccgttg agcatgcgca acttcatctc ttcataaggc agcacatcgc
     5341 taaccagttc ggctcccgct ttttcccatt ccggacgtcc ggcaacaaag ttatcttcta
     5401 ttacccactg gcggaaaggt tcacaggcaa cgcccgcagg atcgcgcaca ccggtaagtt
     5461 gttcgatttt cgccagcgta tcctctgtca ctgcgggcac aatacggtcc accattgttg
     5521 atgggaaagt cacgttatct tcgatccatt gtgccagttt tacatcaacg gcttgcgcgt
     5581 aggaagtgac aacatcacgc ataacatgac cgttttctgg catgttgtca catgacatga
     5641 cggtaaatgc gggaagtcct gccgctttac ggcgagccag cgcctcaaca atcacccctg
     5701 ttgctgtttt cggctggtgg ggattttgca cgtcggcagc gaccatcggg tgatcgagca
     5761 ttaactgtcc ggtcgccgga gagtggaaat accctttttc ggtgattgtc agagagacaa
     5821 tcgcgatttg cggttcacac atcgcagcca acacggtttc taagccatcc atctgtacgt
     5881 gcaaggcttt tttaacgacg ccaacgacgc gagccgtcca cgcatcggcc gacatttccg
     5941 caacggtata aagattatct tgctgttgta aatcggcaat ttgctgttcg ccgccgatta
     6001 agttgaccta ataatatccc cagtcactga aatgttctgt agcaagaata tcggcataca
     6061 cgccctgatg cgcacggtga aatgcaccaa agcctaaatg aacaattctt ggggccaggt
     6121 tattacgatc ataaacaggg agtgtcgctt ttgctgataa caaattattt cccataacaa
     6181 ttccttaaat ttaaatatag caagctacat gttttgttat atgaataaaa atcccctctc
     6241 cggtaagaga agggattgag gatttacaga cttctggaag gttgcgcagc tcttacaaca
     6301 cgcggttgat cttccgcagc gtcttccagc gcacttaaat cacggtcttt cacctctggc
     6361 attttcagcg cagagattaa accaatcact gaatatgcca tgatcataat ggcgatcgga
     6421 taccaggatt ccgtcatggt gcagaaaata cccgccagga taggaccaaa accggaagcg
     6481 ataagaccac caatttcttt agaaatagcc atccgggtaa agcggttttt acagccgaac
     6541 atttctgcca tggtaatgtt ttccagagca aataatccca gcaccgcaca gttatgaatc
     6601 acaatcagtg caaccataat ggtgctcggg gcatagcttt tatctacaat gatagaaagc
     6661 attggccatg ccagcacaat cgcggaggta ttcataataa tatacgggat ccggcgacca
     6721 attttatcgg ataaccaacc aaggaacgga atggtcataa agccgagaat cgaactaatc
     6781 atcaatgcat ctgttggaat tgctttgtta aacaataacg tctgcactaa atagcctgca
     6841 aggaaagtct gaattaaacc tgagttacca gcctgaccaa aacgcagccc tgttgccagc
     6901 cagaaggatt tgctctggaa catgctacca gcaggtgcag gttttgctgt cggttggttg
     6961 ctgtcattaa ccttctcaaa gaccgggctt tctttcagat tcatacgcaa ccagatagca
     7021 aagaccatca cgacaacgct cgccaggaac ggtatacgcc atccccacgc cagcagttcc
     7081 tctttactga gaatgaagaa cataaaggcc cagattgccg ttgcgctcaa ggttccgcag
     7141 ttagttccca tagctacaaa tgaggagata attccgcgct taccttttgg cgcatattcc
     7201 gccagcatcg taccggcacc ggaaatttcc gcacctgcac ccaccccctg aataatacgc
     7261 agcgtcacca gcaagatggg tgcaaaaaca ccaatctgtg cataggtcgg taacacacca
     7321 attaaggtgg tacagatccc catcatggtg atggtaataa agagcacttt tttacgccct
     7381 attctgtcgc ccattttgcc gaaaataaat gctccgacaa tacgcgccac ataacctgca
     7441 ccgtaggttc ccattgccag aattaacgcc attgccgttg atgattcagg aaaaaatatt
     7501 tcatgaaaca ctaacgctgc gccgagcgaa tataactgga aatccataat tcatagatgt
     7561 tttttactat tctgtgggtt tttgggtgtt ttctaagttt tttcagatgg ttgtattttt
     7621 tctaaaaatc cctaatctcg attttgctgt ttatttgagg cctttttatg tcccatatat
     7681 gccccacaga taccccgcag ccaaaatcaa caaaatgcca aaaggttctg ttcctgccct
     7741 gcaacaagaa atgctgcgac gtgtcagtaa acgttatgac gatgcagaag tgatcatcaa
     7801 atccaccagc aacaatggcc tttcagttac tcgcaccgcc gataaagatt ctgcaaaaac
     7861 ttttgttcag gagacgctga aagatacccg ggagtctgct gacgagtggt ttgttcgcta
     7921 attaacgagt aaaatcagta acggctggaa atcattcaat actcacacta tctaaaagtt
     7981 caccagccaa cagcggtaag ttctttcata cgacgtgccg cggatcctat tattctagta
     8041 tgaaatccct aaccgatttc aaattctcca gactgctcag attctctttt atcccacgct
     8101 ggcgctgata aatctcgtca ttgcaatcga cctgcgctat cgctgccgca gttcttccag
     8161 tttctgcatc gataactcca tcttctgagc aataaacctc acctatgcaa tatcttatgt
     8221 caattatttc aattgcatta atatcttgaa tggattacat agagttaatg tatccagtac
     8281 tccctattct cttgcatata atacatgttg caacttacat ctcagcgcta tgaaaaaaca
     8341 ccacctctct ctttatgaaa ttctggattt accaagcgct aatttatcat ttcaatctac
     8401 ctttaagtat tgcatttatc tccctacgag gtcatacttt aggaagttaa atatgaatga
     8461 taatatacct acagcgcgaa atcacaaaca atcgacttgt attacagaaa aaaacatgcc
     8521 tatattttta aacttcacag caggcagtat cttacctgag aatgagctag catctttacg
     8581 ttatattgtg cagc
//
LOCUS       DAZFWP020000189         8593 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFWP020000189 DAZFWP020000000
VERSION     DAZFWP020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.189"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            96..746
                     /gene="csgD"
                     /locus_tag="ACHK0Z_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             96..746
                     /gene="csgD"
                     /locus_tag="ACHK0Z_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU4504288.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPENYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            751..1140
                     /gene="csgE"
                     /locus_tag="ACHK0Z_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             751..1140
                     /gene="csgE"
                     /locus_tag="ACHK0Z_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU4504289.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1165..1581
                     /gene="csgF"
                     /locus_tag="ACHK0Z_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             1165..1581
                     /gene="csgF"
                     /locus_tag="ACHK0Z_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU4504290.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1608..2441
                     /gene="csgG"
                     /locus_tag="ACHK0Z_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     CDS             1608..2441
                     /gene="csgG"
                     /locus_tag="ACHK0Z_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU4504291.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2505..2996)
                     /gene="ycdZ"
                     /locus_tag="ACHK0Z_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             complement(2505..2996)
                     /gene="ycdZ"
                     /locus_tag="ACHK0Z_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4504292.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3098..3652)
                     /gene="ycdY"
                     /locus_tag="ACHK0Z_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             complement(3098..3652)
                     /gene="ycdY"
                     /locus_tag="ACHK0Z_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU4504293.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3676..4413)
                     /gene="ycdX"
                     /locus_tag="ACHK0Z_RS21940"
                     /old_locus_tag="pgaptmp_004388"
     CDS             complement(3676..4413)
                     /gene="ycdX"
                     /locus_tag="ACHK0Z_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU4504294.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4468..5406)
                     /gene="ghrA"
                     /locus_tag="ACHK0Z_RS21945"
                     /old_locus_tag="pgaptmp_004389"
     CDS             complement(4468..5406)
                     /gene="ghrA"
                     /locus_tag="ACHK0Z_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU4504295.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5640..5727
                     /locus_tag="ACHK0Z_RS21950"
                     /old_locus_tag="pgaptmp_004390"
     tRNA            5640..5727
                     /locus_tag="ACHK0Z_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5674..5676,aa:Ser,seq:gga)
     gene            complement(5877..6719)
                     /locus_tag="ACHK0Z_RS21955"
                     /old_locus_tag="pgaptmp_004391"
     CDS             complement(5877..6719)
                     /locus_tag="ACHK0Z_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4504296.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6804..7001)
                     /locus_tag="ACHK0Z_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     CDS             complement(6804..7001)
                     /locus_tag="ACHK0Z_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4504297.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7013..7504)
                     /locus_tag="ACHK0Z_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     CDS             complement(7013..7504)
                     /locus_tag="ACHK0Z_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4504298.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7501..7875)
                     /locus_tag="ACHK0Z_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     CDS             complement(7501..7875)
                     /locus_tag="ACHK0Z_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4504299.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7922..8296)
                     /locus_tag="ACHK0Z_RS21975"
                     /old_locus_tag="pgaptmp_004395"
     CDS             complement(7922..8296)
                     /locus_tag="ACHK0Z_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4504300.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8459..>8593)
                     /locus_tag="ACHK0Z_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             complement(8459..>8593)
                     /locus_tag="ACHK0Z_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HGU4504301.1"
                     /translation="RWHGSAEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTLN
                     "
BASE COUNT         2214 a         2132 c         2079 g         2168 t
ORIGIN      
        1 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
       61 aggtgtgcga tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
      121 atggtcatac attattgttg atcactaaac cgtctttgca agcgacagct ctcttgcagc
      181 accttaaaca atcgctggca attacaggaa aattacataa tattcaacgt tctctggacg
      241 atatatcttc aggctctatt attcttctgg atatgatgga agcagataaa aagcttatcc
      301 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
      361 cgcctgaaaa ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
      421 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgcggcgaat
      481 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
      541 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
      601 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
      661 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
      721 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
      781 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
      841 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
      901 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
      961 agctggatca ctataacggt caatcaggac gttattttcc agactttttt atttccgttg
     1021 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     1081 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     1141 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     1201 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     1261 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     1321 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     1381 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     1441 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     1501 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     1561 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     1621 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     1681 ccagaccgac attaatgcct cgagctcaga gctacaaaga tttgacccat ctgccagcgc
     1741 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     1801 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccacggca atgctggtca
     1861 cggcattgaa agattctcgc tggtttatac cgctggagcg ccagggctta caaaacctgc
     1921 ttaacgagcg caagattatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     1981 gaatcccgct gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt
     2041 atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca
     2101 cgcaatacca gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg agtaccggtg
     2161 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     2221 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg
     2281 aacccgttat gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     2341 atggtatcga ccgtggtctg tgggatttgc agaacaaagc agaacggcag aacgacattc
     2401 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa aagccggaat
     2461 tatcatccgg cattttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     2521 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     2581 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     2641 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     2701 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga
     2761 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     2821 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag
     2881 taggccgtac agcccaggaa gcctgcccag cttagtaagc ccagggaaac agccacccat
     2941 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     3001 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     3061 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     3121 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     3181 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     3241 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     3301 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt ggcatccctc
     3361 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     3421 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     3481 ccatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     3541 cagccagttt cccctcacga atcagggtaa acagcggcac cagtaaagga tcttgcggtt
     3601 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     3661 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     3721 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctggcg gaaaatctac
     3781 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     3841 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcggctaccg cacgacagtt
     3901 gtcttcactg cccttacgtg agtgtaaaaa tgaagaatta ttgatttcca gcgccacctg
     3961 atgtttcgcg gctgcttcag caacggcttt cacatctatt tcatatttgg gatttccggg
     4021 atggcttatt atatgcacat tgccgctggc gatagtggcg atcatcgctt gtgtattggt
     4081 cgctttgtca tgtggtgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     4141 gtcaaacatt ttaccgctgc aatcaatttc accatcaaca tttttaatgt tagcttcgat
     4201 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     4261 ccagtgatgc ggagcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     4321 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     4381 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     4441 aaggatagca ggaatcctga tgctttatta gtagccgcgt gcgcggtcga cttgcccgca
     4501 gaccctctcc cctttttcga gctgggcaat ggtgcgagaa atgtactcca cagcttcagc
     4561 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     4621 actttcaggc ggtaagggtt cacgattaaa aacatccaac attgcgcctt taactttgcc
     4681 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     4741 gagatacgcg ccatccggta atttttcgag taattgttga ttaataatgc cgacggtttc
     4801 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     4861 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     4921 cagcggaaag cgccaggttt gcagactctg agcaacttta ctgcccagta cgcctgcgcc
     4981 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     5041 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     5101 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     5161 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     5221 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     5281 agcataatca gcagagtcat tatctccgct tttccatgct ctgactcttg cctgaggaat
     5341 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     5401 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     5461 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttac
     5521 taaaaccaca cattgattga agtttgaata aacgcgcgat tttttcaaaa agtttgttga
     5581 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     5641 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     5701 ggggttcgaa tccccccctc accgccagat tataagcgca tgttattgtt taacatgcgc
     5761 ttttttattt tcccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata
     5821 tgccccgcga tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat
     5881 actgccagca tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct
     5941 ggttttctga acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca
     6001 tcttcatagc tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg
     6061 tttccccggt tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg
     6121 gcgagtcggt cagcctgttg tccggtgata agatgaaatc cccaccggtc ccatctcacc
     6181 agattgttga cgataatttt actgccaaat ttacaggggc aattggtttt gtaattccag
     6241 ttcagccctc tgaagacgtt gatcacccct cgctcaaaca cctcatcctt attctggtgc
     6301 agttgttcaa atgtgctcag aatgttcgct tcgctgattt ccggaaaatt atcgtactcc
     6361 agtgaccggt accatgtatc acgggcctgc gtatccatta atgacagcat cccggaacgt
     6421 tgcatcaggt cacgccagat ttcgcgatca agattccggg taatggcttt cattgctgtt
     6481 tcgggtttct ccatcagcca gcagccgcag cgaaaatcct gtttcatggc ccagtccggg
     6541 gcagttttac ctccgatgct ttccgtcagc accgagattt cagccagttg tcggataagt
     6601 gtgtcgattt gcaacagtgt actctttcgt acagccagaa tgtggcgaat gctggttgag
     6661 cagataacgt ccgtatgccc gttgaccact tccggacggg agattgtgat atctgacata
     6721 atcgatattc ctttttgcag ataaatgaaa acgccagccc tgtgcaggct ggcgtcagag
     6781 gaatgaagtc tggtgagtga gcttcaccgg ttctggtgca gaagctgcag atgacgcagg
     6841 gtacggacat cctcccgggc ctgttcgata cagggcaaca gtgctgctga atccgtttta
     6901 tcctcatcgt tgtcaaagat aattcccgat ccgcagtcga tattgtcctg cagccacgca
     6961 atcagaatat ccagcgctgt ttccgtggtt aatgatttca tgttgttcct ggttaccagt
     7021 cgaaagtggg taaacccggc aggcattcag cgctggcatc cagatgaatg agactgacgc
     7081 cataacgccg gatgagtgtg acgaccagac gacggaacgt ttttgacagc cccaggcgtt
     7141 taagccgcag aaccgggtat gaccacgcat cggtacgtaa cagatatccg ctacctgtaa
     7201 aatgaatcca ttctgattca ccgaagtcac tggtctggtg tgacagcgag tacaaccagg
     7261 cgttgtccgt ttccgtgata tgtgcggtac tgcagcgtat gccggtaagg gtagcaaatg
     7321 gtggcgtgca ttctgctgag gtctggtcag attcatcaac aacacgaagg gagtagccgt
     7381 tatcgcagac catgttaatc agttcagcga gctcaatacc atcgacgtca acgacaatgc
     7441 gccccatgtt cagtgcctgt acgttaacgc tgtcggcttc cagtgtcagg gccagtttca
     7501 tcatttcgcc tccggatact tacccaaggt aatgttattt accgttctgt aattgtcgcg
     7561 ggtcatcagg ccggtcgccc ggcgagcccg gaggatatca atactgttta ttaactgaga
     7621 gcggggacag gtgctgaatc ccggctggtc ggtacgcacc agcgcgtatt tttccacgag
     7681 aaaattaact gcatcacaca gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc
     7741 atcggcaaac ggtgtgtcat tcagtgtgag gccataatgc tggtccagca gtcggatgag
     7801 cagtgtctgc cagatggtga cgggagacgg gcagcgagat acctcccgta catgtgtgtc
     7861 ggataatgtt ttcatcttaa gttctctgta actggtagtg ctaaaggtga gagcttcggg
     7921 gttatgaggt ggttgcgagt gctggtgtcg gataaacagc cagataaacg taaccacagg
     7981 agccgagggt gtcggcttcg caggtcagcc cttttgcatt cagcgtgacg gtatgctgat
     8041 atcggggatt cagttcaccg ctggtgagca tgagttccag ttgtttcatc agcagcggaa
     8101 aggcctggtc cagatggtat gcatctgcgt cgctgaagcg gcctctgata ccggcgcggt
     8161 cggcaaggta atgcaggcgg ttaccctctt gcaccagacg agcgccaaaa cagggcctca
     8221 ctgtgcaggg aagcccccac caggggcggt cgtgattgtc atcgggatga gttatcccgg
     8281 agagtttgtc tgacacgata aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg
     8341 acaggtcagc cggcgcgctg accggtttcg gtgaagatgt atgcattgat gagaatggcc
     8401 tcatccacag cggcgtctga ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc
     8461 agttcagggt gacactcatc agccggacat acgggccaaa actgtcctta cggcgttctg
     8521 caaacacggc aagaacgccg ggaatatcct gcacttcacg accagtatac gcttcagcac
     8581 tgccgtgcca gcg
//
LOCUS       DAZFWP020000190         8550 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFWP020000190 DAZFWP020000000
VERSION     DAZFWP020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.190"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            200..742
                     /gene="ecpR"
                     /locus_tag="ACHK0Z_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /gene_synonym="matA"
     CDS             200..742
                     /gene="ecpR"
                     /locus_tag="ACHK0Z_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4504302.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            817..1404
                     /gene="ecpA"
                     /locus_tag="ACHK0Z_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /gene_synonym="matB"
     CDS             817..1404
                     /gene="ecpA"
                     /locus_tag="ACHK0Z_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU4504303.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATRTS"
     gene            1466..2134
                     /gene="ecpB"
                     /locus_tag="ACHK0Z_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /gene_synonym="yagY"
     CDS             1466..2134
                     /gene="ecpB"
                     /locus_tag="ACHK0Z_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU4504304.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2160..4685
                     /gene="ecpC"
                     /locus_tag="ACHK0Z_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /gene_synonym="yagX"
     CDS             2160..4685
                     /gene="ecpC"
                     /locus_tag="ACHK0Z_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU4504305.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDHGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4675..6318
                     /gene="ecpD"
                     /locus_tag="ACHK0Z_RS22005"
                     /old_locus_tag="pgaptmp_004401"
     CDS             4675..6318
                     /gene="ecpD"
                     /locus_tag="ACHK0Z_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU4504306.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSQESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6287..6997
                     /gene="ecpE"
                     /locus_tag="ACHK0Z_RS22010"
                     /old_locus_tag="pgaptmp_004402"
     CDS             6287..6997
                     /gene="ecpE"
                     /locus_tag="ACHK0Z_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU4504307.1"
                     /translation="MFRRRGVTLTKALLTAVCMLATPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPECDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7310..7639
                     /gene="ykgJ"
                     /locus_tag="ACHK0Z_RS22015"
                     /old_locus_tag="pgaptmp_004403"
     CDS             7310..7639
                     /gene="ykgJ"
                     /locus_tag="ACHK0Z_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4504308.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7887..8499)
                     /gene="yagU"
                     /locus_tag="ACHK0Z_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /pseudo
     CDS             complement(7887..8499)
                     /gene="yagU"
                     /locus_tag="ACHK0Z_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
BASE COUNT         2281 a         2175 c         2153 g         1941 t
ORIGIN      
        1 tcatcaggaa aaaaacataa atttaaacct aatcgaaata attaaaactt aatctcgttt
       61 aacctatatt gatatgtgct acgtatctta tttacttccg atttactaaa gaaactgaat
      121 gtacctgtaa aaattacagg tttggaaagt agtgacatgg caaaatgatt acagcaggga
      181 ctatgaggtt aaaaaccata tggaatgtca aaaccgttct gataaataca tctggtctcc
      241 ccatgacgcc tacttctata aaggactatc tgaactgatt gtggatatcg acagattaat
      301 ttatctatcg ctggagaaaa tcagaaaaga tttcgtgttt atcaatctca atacggattc
      361 tttaactgaa tttataaacc gtgataatga gtggttatcc gcggtaaagg ggaaacaggt
      421 cgtattgatt gcggccagaa agtcagaagc cttagcaaat tattggtatt acaacagcaa
      481 tattaggggc gtggtatacg ctggactgag tcgtgatatt agaaaagaac tggcctatgt
      541 gattaatggc aggttcctga gaaaagatat taagaaagat aaaatcactg accgagaaat
      601 ggaaattatc cgcatgacgg ctcagggaat gctgcctaaa tcgattgcca gaattgaaaa
      661 ttgtagtgta aagacagtgt atacccatcg gcgtaatgca gaggccaagc tgtactcaaa
      721 aatatataag ttggttcagt aaactccagg caagttagtt ttaaaaaatg actcactggg
      781 acatcacgtc ctcaattcaa ctcgggaaga aatgcaatga aaaaaaaggt tctggcaata
      841 gctctggtaa cggtgtttac cggcatgggt gtggcgcagg ctgctgacgt aacagctcag
      901 gctgtagcga cctggtcggc aacagccaaa aaagacacca ccagtaagct ggttgtgacg
      961 ccactcggta gcctggcgtt ccagtatgcc gaaggcatta aaggttttaa ctcacagaaa
     1021 ggtctatttg acgtggctat cgagggtgac tcaacggcta ccgcctttaa actgacctca
     1081 cgtcttatca ccaacacctt aacccagttg gatacctcag gttctacact gaatgtgggc
     1141 gtggattata acggcgcggc agtcgaaaaa actggcgata ccgtgatgat cgataccgcc
     1201 aacggcgtac tgggcggcaa ccttagcccg ctggctaacg gttacaatgc cagcaatcgt
     1261 accaccgcac aggatggttt cactttctcc atcatcagcg gcaccaccaa tggtaccacc
     1321 gcagtaaccg attacagcac tctaccggaa ggcatctgga gcggcgacgt tagcgtacag
     1381 ttcgacgcga cccggaccag ttaatctctc tgatgtacca gcaggggtag cccccccccc
     1441 tgctttattc ctggacggaa ttgttatgaa aaagcacctt ctgcttctcg ctctgctgtt
     1501 atccggaata tctccggccc aggcgctgga tgtcggcgat atatcatcgt ttatgaacag
     1561 tgacagcagc acgctgagca aaacgatcaa aaacagtacc gacagtggtc gcctcatcaa
     1621 tatccgtctc gaacggctct cttcaccgct tgacgacggg caggttatcg caatggacaa
     1681 gccggatgag ttgctactca ctcccgccag cttgctgcta cccgcccaag ccagcgaagt
     1741 gatccgcttc ttctataagg gaccagcaga tgaaaaagag cgctactacc gcattgtctg
     1801 gtttgatcag gccctcagtg atgcgcagcg cgataatgcc aaccgcagcg ctgtggccac
     1861 tgcttccgcc cgcatcggca ccattctggt cgtcgcaccc cgccaggcaa actaccactt
     1921 tcagtacgcc aacggctccc tgacaaatac aggaaatgcg acgctgcgga tcctcgctta
     1981 cggcccctgc ctgaaagccg ccaatggtaa ggagtgtaaa gagaattact acctgatgcc
     2041 gggcaagtcg cgtcgtttta cccgcgtgga cacggcggat aacaaaggac gggttgcact
     2101 ttggcagggt gataagttca ttcccgtgaa atagatagct gtgcagatgg ataacgacaa
     2161 tgcctttacg acggttctcc ccaggactga aagcccagtt tgccttcggc atggtctttt
     2221 tgttcgttca gcccgatgcc agcgctgctg acataagtgc gcagcaaata ggtggggtga
     2281 ttattccgca ggccttcagt caggcgctcc aggacggcat gagcgtcccg ctttatattc
     2341 atctcgccgg tagccagggt cgccaggacg atcagcgaat cggcagcgct tttatctggt
     2401 tggatcatgg acagctacgc atccggaaaa tacagctgga agagagtgaa gataacgcca
     2461 gtgtcagcga acaaactcga cagcagctga tggctctggc gaacgccccg ttcaatgagg
     2521 cccttaccat ccccctgact gacaacgcgc agctggatct cagcttgcgc caactgctgc
     2581 tgcagctggt ggtcaagcgc gaagcgctgg gcactgtact acgctcacgt agcgaagaca
     2641 tcgggcagtc cagtgttaac accctcagca gtaatctgag ctataacttc ggcatctata
     2701 acaaccagtt gcgtaacggc gggagcaaca catccagcta tctgtcgctg aataacgtta
     2761 ctgcactgcg cgaacatcat gtggtgctcg acggctcgct gtacgggatc ggtagcggtc
     2821 aacaggacag tgaattatat aaagcgatgt atgaacgcga ttttgccggt caccgatttg
     2881 ccggtggaat gctcgacacc tggaacttgc agtccttagg gccgatgacc gccatttcag
     2941 cagggaagat ttacggcctt tcctggggaa accaggccag ctccaccatc ttcgacagca
     3001 gccagtcagc cacgccagtg atcgcctttt taccggcggc gggcgaagta catctcaccc
     3061 gtgatgggcg gctactaagc gttcagaact tcactatggg caatcatgaa gtggataccc
     3121 ggggtctacc gtacgggatt tacgatgtgg aagttgaggt gatcgttaac ggtcgcgtga
     3181 tcagcaaacg cccccagcgg gtcaataagc tgtttagccg ggggcgcggc gtcggtgcac
     3241 cactggcgtg gcaggtatgg ggcggtagct ttcatatgga tcgctggtcg gaaaacggga
     3301 aaaagacgcg accagctaaa gagagttggc tagcaggtgc ctcgacctcc ggctcattga
     3361 gtacgcttag ctgggcggca acgggatatg gatacgataa tcaggcggtg ggtgaaaccc
     3421 gtctgacgct gccgcttggg ggagcgatca acgttaacct gcaaaatatg ctggccagtg
     3481 acagctcatg gagcaacatc gccagcatca gcgccactct accgggaggc tttagttcgc
     3541 tgtgggttaa ccaggaaaaa acccgcattg gcaatcaatt gcgacgtagc gatgccgaca
     3601 accgtgcaat cggcggcaca ctcaacctga actcactgtg gtcgaagctg ggcacattca
     3661 gcatcagcta caatgatgac cgccgttaca acagccatta ttacacggca gattactatc
     3721 aaaatgtcta cagcggtccc tttggttcgc ttggcctgcg ggccggtatt cagcgctata
     3781 acaacggcga cagcaacgcc aatacaggga aatatatcgc tctcgatctc tcgctaccac
     3841 tgggcaactg gtttagcgca gggatgactc atcaaaacgg ctacaccatg gcaaacctgt
     3901 cagcacgcaa gcagtttgat gaagggacca ttcgcactgt tggtgccaat ctgtcacgag
     3961 ccatctccgg cgataccggt gatgacaaaa ccctcagcgg tggggcgtat gcacagttcg
     4021 acgctcgtta cgccagcgga acgctgaacg tcaatagcgc ggcggacggc tacgtcaata
     4081 ccaatttaac cgccaatggc agcgtcggct ggcagggtaa aaacattgct gccagcgggc
     4141 ggactgatgg caacgctggg gtgatattca acaccgggct ggaggacgac ggtcagatca
     4201 gcgccaaaat caacgggcgg attttcccgc ttaacggcaa gcgtaactat ctcccgctct
     4261 ctccctatgg aagatatgag gtggagttac agaacagcaa aaactcactc gacagttacg
     4321 atatcgtcag cggccgcaaa agtcatctga ctctctatcc aggcaatgtc gctgtcattg
     4381 agccagaggt gaagcagatg gttaccgtct ccggccgtat ccgtgcggaa gacggcacac
     4441 tgctggctaa cgcacggatt aacaaccata tcggccgaac ccgaaccgat gaaaacggcg
     4501 agtttgtcat ggatgtggac aagaaatacc ccactatcga ttttcgctac agtggcaata
     4561 aaacctgcga agtggcactg gaactcaacc aggcgcgcgg tgccgtctgg gtcggagatg
     4621 tggtctgcag cggcctctca tcgtgggcgg cggtgacgca gacaggagaa gagaatgaga
     4681 gttaacctac tgatagcgat gataatcttt gcgctaatct ggcctgcaac cgcgctcaga
     4741 gctgcagtga gcaaaacaac ctgggcggat gcaccggcac gcgagtttgt gtttgtcgaa
     4801 aacaactcag acgacaactt tttcgtcact cctggcgggg cgctggatcc gcgcctgacc
     4861 ggtgccaacc gctggaccgg tttaaaatac aatggttcag gaaccatcta tcagcaaagc
     4921 ctcggctaca ttgataacgg ttacaacacc ggcctttata ccaactggaa gtttgatatg
     4981 tggctggaaa attcaccagt ttcatctcct ttaactggct tgcgctgcat caactggtac
     5041 gctgggtgta atatgaccac cagtcttatc ctgccgcaaa ccaccgacgc cagtggattt
     5101 tatggcgcga cggtgaccag cggcggcgcg aagtggatgc acggcatgtt gtcagacgcg
     5161 ttttaccagt atctgcaaca aatgcccgtc ggcagcagct ttacaatgac catcaatgcc
     5221 tgccagacct ctgtgaacta tgacgccagc agcggcgcac gctgtaagga tcaggcctcc
     5281 ggcaactggt atgttcgcaa cgtcacccat acgaaagcag caaatctacg gttgataaat
     5341 acccactcgc tggcggaagt atttatcaac agcgacggag taccgactct gggcgaaggg
     5401 aacgccgact gccggacgca aaccatcggc agccgttcag gattaagttg taagatggtt
     5461 aactataccc tgcaaacaaa cggactcagc aacacctcaa tccatatatt cccggcgatc
     5521 gccaactcgt cgttagcctc ggccgtcggg gcgtacgata tgcagttcag tctgaatggc
     5581 agttcatgga aaccggtgag caatactgcc tattactaca ccttcaacga gatgaagagc
     5641 gcagactcga tctatgtttt cttctcgagc aacttcttta agcagatggt gaacctcggg
     5701 atcagcgata tcaacaccaa agatctattc aactttcgct ttcagaacac cacatcacag
     5761 gagtctggct ggtatgaatt ttctacctcc aacacgctga ttatcaaacc ccgtgatttc
     5821 agcatcagta ttatctccga tgaatatact cagacaccgt cgcgggaggg atatgttggc
     5881 agcggcgagt cggcactcga tttcggctat atcgtaacca ccagcggtaa aacagctgcc
     5941 gacgaagtgc tgatcaaggt gaccggaccc gcgcaggtga ttggcgggcg ctcctattgt
     6001 gtcttcagct ccgatgacgg taaggcgaaa gtaccgttcc cggcgacgct ttcctttatt
     6061 acccgcaacg gagctacaaa aacctacgat gccgggtgcg atgatagctg gcgtgatatg
     6121 accgatgcgc tgtggctgac cacaccgtgg actgatatct ctggcgaagt ggggcagatg
     6181 gataagacca cagtcaaatt ttcgattcca atggataacg ccatttcgct gcgtacggta
     6241 gatgataacg gctggtttgg cgaagtcagc gcttcaggag aaattcatgt tcaggcgacg
     6301 tggcgtaaca ttaactaagg ccctgctgac agcggtctgt atgctggcga cacctttgac
     6361 acaggcgatt tcggtcggca atctgacatt ttcgctgccg tccgagactg actttgtcag
     6421 caaacgtgta gtgaataaca acaaaagcgc acggatatac cgtattgcca tcagtgctat
     6481 tgatagcccg ggcagcagtg aattgcgcac ccgaccggtg gatggtgaac tgcttttcgc
     6541 cccccgccag ctagcgttgc aggctggtga gagcgagtat tttaaatttt actatcatgg
     6601 gccacgggat aaccgcgagc gctactaccg ggtctcattt cgcgaggtcc ccactcgtaa
     6661 ccagacaaga cgtagcccaa ccggcggcga ggtcagcacg gagccggtgg tggtgatgga
     6721 taccattctg gtagtacgac cacgtcaggt tcagtttaaa tggtccttcg acaaggtgac
     6781 aggaacggta agtaacactg gcaacacatg gtttaagcta ctgattaagc cagaatgtga
     6841 ttcgaccgaa gaggaaggcg atgcctggta tctacgtccg ggagacgtgg ttcatcagcc
     6901 tgagttacgt cagccgggga atcattatct ggtctataac gacaaattca ttaagattag
     6961 cgattcctgt ccggctaagc ccccttcggc ggactaagac ttatccatcg gtcgagggaa
     7021 gaattccacc tcagagcccc aaaaatcgtt taaatgatgt ggaagcgatc gtcaatatca
     7081 cctgtaacga tgggctttct catatgcaca ccatagtgtt caggagacac ggaaaggtat
     7141 caggcagcct gctaacgagt ggtttaatca cgaattagta cgtaaaatcg gtaacggctg
     7201 gaaatcattc aatactcaca ctatcgaaag ttccccagcc aaccgcggta cgttcttaca
     7261 tacgatgtac cgctgttctc tttacgattt ttagctgtac tggtgaatta tgagcaatct
     7321 gaatccatgc atgacgtgtg gtgcctgttg tgcatttttc cgcgtctctt tttactgggc
     7381 cgaagccgac gatgctggcg gcactattcc cgccaggctc actgaacaaa tatccccttt
     7441 tcaccgatgc atgagcggta ccaatcagaa aaacccccga tgtattgccc ttgcaggaac
     7501 cccgggcaaa aatgcctgct gcacgatata taaaaatcga tcgtccacat gcagagaatt
     7561 tgccatgtct ggtgagaacg gagtcgtcaa tgaggcttgc aatcgtgcaa gggctaaata
     7621 cgggctgaca ccgttataaa catacaataa ttaattgcac tgccccgcca gcgataatag
     7681 cggggcttcg tttttcaggg gtaacaaaac ccgatacttc ttctatttgc cagcaacaat
     7741 gcctctcttc tgtagcgttc ttcggatcta catcatcctg aagtagcgcg atatactcac
     7801 tgactatctg aatcagaata taaaaagcaa tgtttttaac ctataaaaat ggcgctgtat
     7861 ttgcgccatt tttatcattc aatgcattat ctgtttgagc ctaaagggat ctcagggtct
     7921 ggctcatgag taattctgtt tcgtaaatct ctgcgaataa tttcaataga ccagaaccag
     7981 actaaatgtc caaaaatttc agaaacattc tcataccacg ggagatcaaa cagaggtggc
     8041 gtcagtccca tgagagggaa tgaaatcata tgaacaaaaa gttgggctaa agcacctgcc
     8101 agtaagccct gccagagttt aatttttgga aatacttcag cgaccacaca ataaccgaca
     8161 gcaaacacta tcgaaaagat aatgtgcgta acaccaaccc agttaaagac atgcccggca
     8221 aaggtataaa cagccgcatt gggatctgtc agccccaacc agtctcgaag aaaaatatac
     8281 ggtggattga gaaaattacg cgagcaatca atttggcctg cagccctgat taatgattcc
     8341 gggccacacg ctgcattaaa catatccacc gggctacgtg gcggcaatgg aacttcagcc
     8401 ccacttcacg aatgcggaaa caacgccagc aatcagccca atgaatgcag caagaccata
     8461 acgtctgcgg ttcggtggag tttgttcaaa tatattcata tctaccctgc ttgtaccatt
     8521 atgttataca cctcttcagg agtattcata
//
LOCUS       DAZFWP020000191         8488 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFWP020000191 DAZFWP020000000
VERSION     DAZFWP020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.191"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..402
                     /locus_tag="ACHK0Z_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             <1..402
                     /locus_tag="ACHK0Z_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504309.1"
                     /translation="LRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDNLRTFNTNEL
                     TRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQE
                     TLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            625..1122
                     /gene="ubiC"
                     /locus_tag="ACHK0Z_RS22030"
                     /old_locus_tag="pgaptmp_004406"
     CDS             625..1122
                     /gene="ubiC"
                     /locus_tag="ACHK0Z_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU4504310.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1135..2007
                     /gene="ubiA"
                     /locus_tag="ACHK0Z_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             1135..2007
                     /gene="ubiA"
                     /locus_tag="ACHK0Z_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU4504311.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2162..4585)
                     /gene="plsB"
                     /locus_tag="ACHK0Z_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             complement(2162..4585)
                     /gene="plsB"
                     /locus_tag="ACHK0Z_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU4504312.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4756..5124
                     /gene="dgkA"
                     /locus_tag="ACHK0Z_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             4756..5124
                     /gene="dgkA"
                     /locus_tag="ACHK0Z_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4504313.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5234..5842
                     /gene="lexA"
                     /locus_tag="ACHK0Z_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             5234..5842
                     /gene="lexA"
                     /locus_tag="ACHK0Z_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU4504314.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5915..7240
                     /gene="dinF"
                     /locus_tag="ACHK0Z_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             5915..7240
                     /gene="dinF"
                     /locus_tag="ACHK0Z_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU4504315.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7356..7565
                     /gene="yjbJ"
                     /locus_tag="ACHK0Z_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             7356..7565
                     /gene="yjbJ"
                     /locus_tag="ACHK0Z_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU4504316.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7607..8122)
                     /gene="zur"
                     /locus_tag="ACHK0Z_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             complement(7607..8122)
                     /gene="zur"
                     /locus_tag="ACHK0Z_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU4504317.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1955 a         2042 c         2299 g         2192 t
ORIGIN      
        1 ttacgatcaa cggatgatat gatcaaagca aatgcctggt atctttataa agagtggatt
       61 cgtagtgatg atgtttcacc tctatttata gaaattgaag ataatttaag aacctttaac
      121 acgaatgaat taacacgaaa ggataatatc tttatcctgt tctcctctgc cgatgatggg
      181 ccagttatgg tggtaagctc ccagcgctta catgatatgt tgaatcctac aaaagatacc
      241 aattggaatt ccacgtgtat ctataaatcc agacataaga tgttgcctat taatcttact
      301 caggaaacac tttttagctc caaatctcat ggtaaatatg cgcttttccc aatttttact
      361 gcgagttggc gagctactcg tataaagaat ataggtattt aagtaaagga aaacatcacc
      421 gttcctggca tcatgggcgg tgatgcccct acggttgccc tcgccagcac gggcatcggc
      481 aaagcgtaag gttcaacagc ggtttaccac ttcatgcgat tgttgcgttt ttgttgcgca
      541 ttagatcact taatttgctt tacatctccc gtaaacactt ttctgcgata caatgccttt
      601 acgttatgta acggagagtt cggcatgtca caccccgcgt taacgcaact gcgtgcgctg
      661 cgctatttta aagagatccc tgccctggat ccgcaactgc tcgactggct gttgctggag
      721 gattccatga caaaacgttt tgaacagcag ggaaaaacgg taagcgtgac gatgatccgc
      781 gaagggtttg tcgagcagaa tgaaatcccc gaagaactgc cgctgctgcc gaaagagtct
      841 cgttactggt tacgtgaaat tttgttatgt gccgatggtg aaccgtggct tgccggtcgt
      901 accgtcgttc ctgtgtcaac gttaagcggg ccggagctgg cgttacaaaa attgggtaaa
      961 acgccgttag gacgctatct gttcacatca tcgacattaa cccgggactt tattgagata
     1021 ggccgtgatg ccggactgtg ggggcgacgt tcccgcctgc gattaagcgg taaaccgctg
     1081 ttgctaacag aactgttttt accggcgtca ccgttgtact aagaggaaaa aaatatggag
     1141 tggagtctga cgcagaataa gctgctggcg tttcatcgct taatgcgtac ggataagcca
     1201 attggcgcgt tactgctgct ctggccaaca ttatgggcgt tgtgggtggc gacaccgggc
     1261 gttcctcagc tctggatcct ggcggtattt gtcgcgggtg tctggctgat gcgcgctgct
     1321 ggatgtgtgg tgaatgatta tgctgaccga aagtttgatg gtcatgttaa gcgcacggcg
     1381 aaccgaccac ttcccagcgg cgcggtaaca gagaaagagg cgcgcgcact gtttgtagtg
     1441 ctggtactga tttcgttttt actggtactg acgctgaata cgatgaccat tctgttgtcg
     1501 attgccgctc tggcgttggc gtgggtgtac ccgtttatga agcggtatac ccatctcccg
     1561 caagtggtgc tgggcgcggc gtttggctgg tcgattccaa tggcttttgc cgctgtgagt
     1621 gagtcggtgc cattgagttg ctggttaatg ttcctcgcca atattctctg ggcggtggct
     1681 tacgacacgc agtatgcgat ggttgaccgc gatgatgatg tgaagattgg cattaaatcc
     1741 acggcaatcc tgttcggcca atacgataaa ttgattattg gtattttgca gattggcgta
     1801 ctggcactga tggcgatcat cggtgagtta aatggcttag gctggggata ttactggtca
     1861 attgtggtgg ctggcgcgct gtttgtttat caacaaaaac tgattgccaa ccgcgagcgt
     1921 gaagcctgct ttaaagcatt tatgaataat aactatgttg gtctggtact atttttaggg
     1981 ctggcaatga gttactggca tttctgatga tgtaaaaaag ccggatgatc atccggcttt
     2041 cttctgggtt gcctgatgcg cggcgcttct caggcctaca caacacatcg caatttattg
     2101 aatttgcaga ttatggaagg ccggataagg cgttttcgcc gcatccggca attctctctg
     2161 attacccttc gccctgcgtc gcactctcaa tcgtcaaacg cacgtctgat gtaatcaact
     2221 ccgccagcaa ctgataaacc ttcatcgttt ctgccggttc ggcgtcgccg ctatcgctga
     2281 tatacccttc atcacgcagc gtcagcacca gagaactgaa caccgccttg tcgaagaact
     2341 ccggcgcgtt gatgccgtgc agcacggaga gacgttgcgc gacggtgcgg ctctctttct
     2401 ccagcgtacc gcggttgatc gacgggttgg cactcaacaa ccagaaggtg atggcataac
     2461 gttgcagcgt ttcgcgcgcg cctgcggcca gcagctgtag ggtgcgagaa tgcgccgggt
     2521 tgatatgcaa ctcatcatct tgcagggtaa tcagcccctg acgttgcatc tcatttgcca
     2581 gcgcatcaat aacgtccggc aactcgtcgc gatcccagcg caggaacagc tccgctttca
     2641 gcattgggta aagcacattg acgtgctcca tcaatacgtc gcgggagatg tggcgatgct
     2701 gggtgacaat tgccgccatc agcgaaggca gcaccaacat atgcgcaatg ttgttgcgat
     2761 agtaggtcat cagcaccgct tgctcgcgtg gcagaatgat gatgtcgccg atagtgtctt
     2821 tctcgacttc aaacttgttc atttgcagcg cgtgatcgat aagctcgctg gcgctggctg
     2881 aaggaacggt agagtccgtg gagtaaggta cgttgcgcat cagatccagg tagcagttga
     2941 gttgctcggt taactgctcg cgggtgagtg agcgctgacg tgatgccagt agcgcagtac
     3001 agcacaggtt catggcgttt gccgcgcctg cgttgttaat gcgtaccatc agatcggcag
     3061 caatattatt gaccgtcggc gttaaccagg ccggacgcac cgcttcgatg ggatcgatag
     3121 attcacgcca gtctggtacg tgctggttaa ggtaggtcat caacggcatt ggttcaccga
     3181 agttgacgta accctgaccg agattacgca gcttgcttaa accgcgcagc atctgcggca
     3241 ggctctcttt ctctttcgtc gcgccgcgca gttctttggc gtaagtaccc acttccatga
     3301 cgtgctcata accgatatag atcggaatca gcgtaatcgg acgcgtgccg ccacgcagca
     3361 tcgcctgaat ggtcatcgac agcgtaccgg ttttcggatc cagcaaacgc cccgtgcggg
     3421 aacgaccgcc ttccacgaag tactcgacgg aataaccacg gctgaacagt tcgccgagat
     3481 actcacggaa aacggtggaa taaagtttat tgcctttaaa agtacggcga ataaagaacg
     3541 cccccagacg gcggaaaatc ggcccggccg gccagaagtt caggttgatc ccggcggcga
     3601 tatgcggcgg caccagcccc tggtggtaca gcacgtaaga aagtagcagg tagtccatgt
     3661 gactgcggtg gcaaggcaca tacaccagct catgaccgtc gtgggccagc tggcgaacgc
     3721 gctcggcgtt atggacgttg atgccctgat aaagtcggtt ccaggtgaag cccagaatgc
     3781 ggtcagtcag gcggatcatc tcgtaagaga aattcgccgc aatctcttcc atcagcgcaa
     3841 tcgcgttctg ctgcgctttt tcatgggaga tttttttgct gcgcgcttca tcttctaccg
     3901 ctttggcaat ggcgcgggag gcgagcagct tattaaacag atcctgacga gcaggcagac
     3961 gtgggcccac agcagccaga cgttgacggg caaagtgcat acgcgccacg cgcgccagtt
     4021 tctgtgcgat agttttatcc gtgccgtgtt catccgccat acggcgcagc gaaactgacg
     4081 gcgagaaacg cacaaaactg tcgcgaccga gccacagtac ggcgaaaaat ttctgtacgc
     4141 cgttaagcat acgcagcggc gggtttactt cgcctttttc acgccccggc gcgcgaccaa
     4201 acatcaccga tactggcacc atctgcacat ccagatttgg gttgctacgg tgcaaatcga
     4261 gatagtcgtg gaacagctta atagactctt ctttcggcgt gtaataggtg aatacacgcg
     4321 gcccgccgtg aatgaacaca tagcgcggca gtagcgtgcc gtcgatttcc agcggctcta
     4381 acgggtcagg caagtcatga gccagacact gggcgcgtaa cgtcagcaaa tctgctttcg
     4441 agttgtacgg taaaacgtac ataattggac gagaagtatc cagccccagt tccggggcag
     4501 gatctgccgg aatagacttg ctttttacca ggatgcttaa tggtaaattc agtaatttgt
     4561 agtaaattcg tggccagccg gacataaacg atgtaaagcc tctggttaat aatgcaaatg
     4621 cgcggcaagg atagcagaaa gtcatgggaa attctgtggt atccgctcat gtttcgcgcg
     4681 gcgttacgca aacccgaatc atcggattta acggtacact gatattgacg ctcataatgt
     4741 aaaaaggttc tttcaatggc caataatacc actggattca cccgaattat caaagctgct
     4801 ggctattcct ggaaaggttt acgcgctgca tggatcaacg aagcggcatt ccgtcaggaa
     4861 ggcgtagcgg tattgttggc ggtggtcatc gcctgctggc tggatgtgga cgcaattacc
     4921 cgcgtgctgc ttatcagctc cgtgatgctg gtgatgattg tggaaattct caatagcgcc
     4981 atcgaagcag tggttgaccg aattggctct gaataccatg agctttccgg acgcgctaaa
     5041 gatatgggat ccgccgcggt gctgattgcc attatcgtcg ccgtgattac ctggtgcatt
     5101 ctgttatggt cgcattttgg ataacccttc cagatttcga taaatctctg gtttattgtg
     5161 cagtttatgg ttccaaaatc gccttttgct gtatatactc acagcataac tgtatataca
     5221 cccagggggc ggaatgaaag cgttaacggc caggcaacaa gaggtgtttg atctcatccg
     5281 tgatcacatc agccagacag gtatgccgcc gacgcgtgcg gaaatcgcgc agcgtttggg
     5341 gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg ctggcacgca aaggcgttat
     5401 tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg caggaagagg aagaagggtt
     5461 gccgctggta ggtcgtgtgg ctgccggtga accgcttctg gcgcaacagc atattgaagg
     5521 tcattatcag gtcgatcctt ccttgttcaa gccgaatgct gatttcctgc tgcgcgtcag
     5581 cgggatgtcg atgaaagata tcggcattat ggatggtgac ttgctggcag tgcataaaac
     5641 tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt gatgacgaag ttaccgttaa
     5701 gcgcctgaaa aaacagggca ataaagtcga actgttgcca gaaaatagcg agtttaaacc
     5761 aattgtcgtt gaccttcgtc agcagagctt caccattgaa gggctggcgg ttggggttat
     5821 tcgcaacggc gactggctgt aacatatctc tgagaccgcg atgccgccag gcgtcgcggt
     5881 ttgtttttca tctctcttca tcaggcttgt ctgcatggca ttcctcactt catctgataa
     5941 agcactctgg catctcgcct tacccatgat tttctccaat atcaccgttc cgttgctggg
     6001 actggtcgat acggcggtaa ttggtcatct tgatagcccg gtttatttgg gcggcgtggc
     6061 ggttggtgca acggcgacca gctttctctt tatgctgttg ctgtttttac gcatgagcac
     6121 caccgggctg actgcgcagg cttatggtgc caaaaatcct caggcattag cccgtgcgct
     6181 ggtgcaaccg ttgctgttgg cgttgggggc tggggcgtta attgcgctgc tgcgtacacc
     6241 gattatcgat ctggcgctgc atattgttgg cggtagtgaa gcggtcctgg aacaggcgcg
     6301 gcgctttctt gaaatccgct ggttaagcgc tccggcgtcg ctggcgaatc tggtattact
     6361 cggttggtta ctcggcgtgc aatatgcccg tgcgccagta attttgttag tggtcggcaa
     6421 tatcctcaac attgtgctgg atgtctggct ggtgatgggg ctgcatatga acgtgcaggg
     6481 cgcggcgctg gcgacggtta ttgcggaata tgcaacattg ctgattggtc tgctaatggt
     6541 gcgtaaaatc ctcaaactac gcggaatttc cggcgaaatg ctgaaaactg cctggcgagg
     6601 aaacttccgt cgcttgctgg cgcttaaccg cgatatcatg ctgcgttcgc tgttgttgca
     6661 actctgtttc ggcgcgatca ccgtacttgg tgcgcgactg gggagtgaca ttgtcgctgt
     6721 taacgcggtt ctgatgacgc tactcacctt taccgcctat gcgcttgatg gttttgccta
     6781 cgcggttgaa gcgcactccg gtcaggcata cggtgcgcgc gacggtagcc agttgctgga
     6841 tgtatggcgg gcagcgtgcc gccagtcggg gattgtagcg ttactgtttt cggtggttta
     6901 tttgctggca ggggaacaca tcattgcgtt gctgacgtcg ttaacccaga ttcagcagct
     6961 ggctgaccgc tatcttatct ggcaggtgat tttgccgttg gttggcgtct ggtgttatct
     7021 gctggacggc atgtttatag gcgcaacgcg tgccgccgaa atgcgtaaca gtatggcggt
     7081 ggccgccgca ggttttgcgc tgacgctcct tacgctgccg tggctgggta atcatggttt
     7141 gtggctggca ttaaccgtct ttctggcgtt gcgcgggctt tctctggcgg ctatctggcg
     7201 gcgtcactgg cgcaacggta cctggtttgc cgcaacgtga cggttaaaaa ttctgaataa
     7261 attatcctaa gccaaattgc tgactacact taatctcacg ttcagaagaa aagtgaacgt
     7321 actctcattc acaacctaac gatgaggtct tgattatgaa taaagatgaa gccggcggta
     7381 actggaaaca gtttaaaggt aaagtgaaag agcaatgggg caaactgacc gatgatgata
     7441 tgacgatcat tgaaggtaaa cgtgatcaac tggtcggtaa aatccaggaa cgttatggtt
     7501 atcagaaaga tcaggcagaa aaagaggtcg tggattggga aacccgcaat gaatatcgct
     7561 ggtaattaat ccctcctgcc cgacgtgtac aaggatgtac gccctcttaa cgcggtttct
     7621 ttttcacctg cacagagtga tcatgctggc actgttcagg atgacgacac gcttccactt
     7681 ccacgcatgc cgcacagagc ccatgtgctt caatcacatt atgccgcagg gcaaacccca
     7741 tttttgccgc cagcgtatgc ataatgtctt ccacgccttc tgcacactct tctttcaccg
     7801 cgccgcagcg atcgcatata aacatggctg acgtatgcgt gggctggtcg aacagatgac
     7861 agagcacata actgttggtg gattccacct tatgcacaaa accttgctca agcagaaaat
     7921 ccagcgcgcg ataaaccgtt ggcggcttgg cttgcggttc agcttcgcgc agtaaatcaa
     7981 gcagatcata agcgctgata gcgccatcct gcagactcat caggcgcaac acttccaggc
     8041 gctgtggggt caggcgcaca ttacgctgcg cgcagatttt ttcagcctgc gctaataact
     8101 cctgcgttgt ggtcttttcc atcgggcacc tcaaagtggg ggttaagaat gtccttactt
     8161 taccatgttc caggaaaaac accgagaacc cttattgttg ccgtaatgtt gattttctgt
     8221 tttgtaggta aggtgttgtg ttgccttgtc gtaccattat caacacgata ataattaata
     8281 attcattttt aaataaattg cttcaagtta actcgctatt tatttaactg ggtttaaacg
     8341 catacctgta gttcagaaat tatgagcgat ttttgatagt gaccagatgg caaaagaatt
     8401 ataaggagga cacggtacga caaaaaaggt agatgaatca tgttgaagat aatacccggt
     8461 gcaactggat actttaataa aacactca
//
LOCUS       DAZFWP020000192         8484 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFWP020000192 DAZFWP020000000
VERSION     DAZFWP020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.192"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            172..2550
                     /gene="ppsA"
                     /locus_tag="ACHK0Z_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             172..2550
                     /gene="ppsA"
                     /locus_tag="ACHK0Z_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU4504318.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2607..4253)
                     /gene="fadK"
                     /locus_tag="ACHK0Z_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             complement(2607..4253)
                     /gene="fadK"
                     /locus_tag="ACHK0Z_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGU4504319.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCVRLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4310..4603)
                     /gene="ydiT"
                     /locus_tag="ACHK0Z_RS22080"
                     /old_locus_tag="pgaptmp_004416"
     CDS             complement(4310..4603)
                     /gene="ydiT"
                     /locus_tag="ACHK0Z_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4504320.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4600..5889)
                     /gene="ydiS"
                     /locus_tag="ACHK0Z_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(4600..5889)
                     /gene="ydiS"
                     /locus_tag="ACHK0Z_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4504321.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5945..6883)
                     /gene="ydiR"
                     /locus_tag="ACHK0Z_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             complement(5945..6883)
                     /gene="ydiR"
                     /locus_tag="ACHK0Z_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU4504322.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6903..7667)
                     /gene="ydiQ"
                     /locus_tag="ACHK0Z_RS22095"
                     /old_locus_tag="pgaptmp_004419"
     CDS             complement(6903..7667)
                     /gene="ydiQ"
                     /locus_tag="ACHK0Z_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU4504323.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASGIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7983..8429
                     /locus_tag="ACHK0Z_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /pseudo
     CDS             7983..8429
                     /locus_tag="ACHK0Z_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT         2039 a         2188 c         2191 g         2066 t
ORIGIN      
        1 tggaaattgt cgaatagagc aggtttgcgc aacgctggga tcagtcttaa aaagtaaaaa
       61 aatatatttg cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc aaagataaat
      121 gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgttc gatgtccaac
      181 aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga tgtagacagg
      241 gttgggggca aaaatgcctc cctgggtgaa atgattacta acctttccgg aatgggtgtt
      301 tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct ggaccaaagc
      361 ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga tgttacccag
      421 cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt ccagcctgag
      481 ctggaaaacg ccatccgcga agcctatgca cagctttccg ccgatgacga aaacgcctct
      541 tttgcggtgc gctcctccgc caccgcagaa gatatgccgg acgcttcctt tgccggtcag
      601 caggaaacct tcctcaacgt tcaggggttt gacgccgttc tcgtggcagt gaaacatgta
      661 tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac
      721 cgtggcgtgg cgctctccgc cggtgttcaa cggatggtgc gctctgacct cgcatcatct
      781 ggcgttatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt tatcacttcc
      841 gcatggggcc ttggggaaat ggtcgtgcag ggtgcggtta acccggatga gttttacgtc
      901 cataaaccga cactggcggc gaatcgcccg gctatcgtgc gccgcaccat ggggtcgaaa
      961 aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa aatcgaagac
     1021 gtaccgcagg aacagcgtga catcttctcg ctgaccaacg aagaagtgca ggaactggca
     1081 aaacaggccg tacaaattga gaaacactac ggtcgcccaa tggatattga gtgggcgaaa
     1141 gatggccaca ccggtaaact gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc
     1201 ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc cgaaggccgt
     1261 gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtca tccatgacat cagcgaaatg
     1321 aaccgcatcg aacctggcga cgtgctggtt actgacatga ccgacccgga ctgggaaccg
     1381 atcatgaaga aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg tcacgcggcg
     1441 atcatcgctc gtgaactggg cattccggcg gtagtgggct gtggagatgc aacagaacgg
     1501 atgaaagacg gtgagaaagt cactgtttct tgtgccgaag gtgataccgg ttacgtctat
     1561 gcggagttgc tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc ggatctgccg
     1621 ttgaaagtga tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc ctgcctgccg
     1681 aacgaaggcg tgggccttgc gcgtctggaa tttatcatca accgtatgat tggcgtccac
     1741 ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag
     1801 atgatgaaag gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc
     1861 gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc tgattttaaa
     1921 tcgaacgaat atgccaacct ggtcggtggt gagcgttacg agccagatga agagaacccg
     1981 atgctcggct tccgtggcgc gggccgctat gtttccgaca gcttccgcga ctgcttcgcg
     2041 ctggagtgtg aagcagtgaa acgagtgcgc aacgacatgg ggctgaccaa cgttgagatc
     2101 atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg tggttgaaga actggcgcgt
     2161 caggggctga aacgtggcga gaacgggctg aaaatcatca tgatgtgtga aattccgtcc
     2221 aacgccttgc tggcagagca gttcctcgaa tatttcgacg gcttctcaat tggctcaaac
     2281 gatatgacac agctggcgct cggtctggat cgtgactccg gcgtggtgtc tgaattgttc
     2341 gatgagcgca acgatgcggt gaaagcattg ctgtcgatgg ctatccgtgc cgcgaagaaa
     2401 cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg accatgaaga ctttgccgca
     2461 tggttgatgg aagaggggat cgatagcctg tctctgaacc cggacaccgt ggtgcaaacc
     2521 tggttaagcc tggctgaact gaagaaataa aataaatccc cggcggcgtt cagtcgccgg
     2581 gtttatgtga tccccgaaga tgaaacttat tcaatctctt cacagacatc ctgcgttaat
     2641 cgccgcataa tatcttttct taacaaaaac ttttgtattt tacctgaggc agtgcgcggt
     2701 agtttttcga ttaccacgat atgttcagga tatttatatt ttgcgacccg tttacggcta
     2761 aaaaaagcca ccacctcttc cagcgataat gaatgatgcg gcgctttcag cacgacataa
     2821 gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa ccacacaggc atcgtgaatt
     2881 ttaggatgct gcaataaaat atcttccact tcacggctgc taatattttc gccgccgcgg
     2941 acaataatat cttttttgcg tccggtaatt tttatatagc cagcctcatc catacggcag
     3001 agatcgccgc tgtaatacca gccttcttca tccagcgcac gggcggttaa ttcaggttca
     3061 tcaaaatatc ccataaacac attggggcca cgcgaggctt cttcaccttc gcaacctggc
     3121 ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc agcgtaacca
     3181 tcggtgtgca taaagcgcga caaaggatca tcgagattca ccaccgcatg cggcgaactt
     3241 tctgtggaac cataaacact taataattta atgccgagct gctggcattc acgcgccact
     3301 tttttgggga ttgtggttcc gccgcaaaga aagaaacgca gcgctgacag gtccgcgggt
     3361 tgtttctcta gtacattcaa aagatcatag acaaacggcg ttgcgcctag catacaggtg
     3421 caacgctgct gctcaagcag cgcgagacac gcatcaggag tgaaaatatc taacaacacg
     3481 ctgcgagcac caattaaaaa tggtgctgtt acgccatgca gaaatcccgt tgcgtgacca
     3541 agtggcgcag gcatcataaa gacatcctgc caggtcagat tcagccgcac gcaataagcc
     3601 cgctcactgg cgagaatatt gttatgcgtt agcattacgc cctttggcag accctcggtt
     3661 ccggaggtaa acagcaccgc agctaattca tcgccgtggg tcgttatcgc cgtggtcagt
     3721 gaggtattgt cggcgataat ctgacttaat gagagggaag aggtggcggg agccagtttg
     3781 tccacgccga caatttgttg tagttgtgga agctgatttt gcagcggcag gattaaatct
     3841 accgggcgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg acacttattg
     3901 agcacccaca ccagttctgc ttcccgccag gaaggcaaca gcggcacgga aaccgcaccg
     3961 attttcaggc aggcaagata gataacggta aattcacacc agccaggcag ttgaaatgcg
     4021 atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca gctcgcggcg
     4081 tgatcgagcg cgctataggt gtacgatgca ccatgattat cgaccacggc aattttgtct
     4141 ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc ccatagccct
     4201 tgctgacgat acgccgcacg acgttgttcg ttgaacgtta atgtcacttt catgttggac
     4261 tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg gatgcatgat tagccgtagc
     4321 gaaaatcaat accgaaggtg cctgcgggat attgccactg ttcgagaata gtgttaccgc
     4381 acagcacccg acaggtgccg cactccagac aaccggcaga atcaaaatga atgttgccgg
     4441 cgtcatcctg cttataaagt ccggcagggc aggctttcat taatttacgg aattcattga
     4501 tatcgggatt tgccgccaga atgatatgcg ggtggccctc atcaacatgg aatttattga
     4561 cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg ttgctccctt
     4621 aatgccatct ttcagcaagt tgatcagccc aattttcttc gcatgtccca tgatcatttt
     4681 gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc tcgttcatga tgtcggcgac
     4741 cattcgtggg tattggctaa acaggcgcgg gttttccatc agcgccggga tcttgcggaa
     4801 atgctgcata tcgcgcatga cgcagctttg ttccagctca cgtttgtatt gcgccagact
     4861 gctggcggag aaatcctcgc gttctttggc ggcgatcacc gttgtggcgg cagcctgagc
     4921 cgatgcaatg gctaaatcca tgccacgaac tgtaaaaccc aaattcaggc agaagcctgc
     4981 ggcgtcacca acgatcatca cgccatcgtt aaccatctgc ggcaccattg ccagaccgcc
     5041 ttctggcacc atatgcccgg aatattcaag cagtttgccg ccgctaatta gtgggcgaat
     5101 ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg cttttttgcg catgggcgat
     5161 atcacccaat ccacaaacca gccccaagga tatggaatcc ttgttggtat agagaaatcc
     5221 cccgcccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac cttcctcgcc
     5281 cgtaatatta aagcgatcgt tgatctgttc tggtgtgagg ccaataacct ctttaacacc
     5341 aacagcgtaa tgatgcggat cggaagcggg aaccattccc agcgagcggc caagcatcga
     5401 gttaacgcca tcagccagaa tcaccacatt cgcttcgaga atatcatccc cagcctgcac
     5461 gccagtgacc ttgtttcctt cacgaaccag cgcatcgacg cgaacgcctg ggataaactg
     5521 cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc agacgattac gcaatacggt
     5581 ataagatgcg tgttgcggaa catctggttg ctcgcggtga aaatcgaggg taacggcgct
     5641 ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg gcgctgatac
     5701 tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa agacgcccgc cggtcatgtt
     5761 tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg ctcgcgccat
     5821 gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc acaatggcat caaatttgtc
     5881 ttccgacatg cgttatgctc ccttcccgct gcgtttacag cgggagatgt ggttgaaagg
     5941 ggaattagcg gctcaactgg ctaatcaggg cagggacgac tttgtagata tcgcccacca
     6001 gaccgtagtc ggcataattg aagattggcg catttttatc tttattgatg gcgacaatca
     6061 cttttgcgcc gttgccgcca accatatgct ggatctgccc ggagatcccc agcgtcaggt
     6121 agagatcgga tttcagcaac acgccggaga caccgatata acgttcacgc tccatccagt
     6181 tctcgccttc ggcaattgga cgtgaacagc cgacttcagc attcagcacc gccgccagtt
     6241 cgtggaccat tttcaggtca tcctgcgccg ccagaccacg accgacgcca accacacgtt
     6301 ttgctttgct caggtccacg ctgcttgcgg ctttcgcacg gcgttcgcga cagagaattt
     6361 catgacgcgg agcaacataa ggtaccgttt ctgtcgggca ctgatgagag gtatcactgg
     6421 tgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc cagcgggctg ttgatctttt
     6481 cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga ccatcgataa
     6541 tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag ttgcacactt aaccgtgctg
     6601 ccagagcttt accacgtttc gtcgcggcca acagcaacat agcggggtgt ttatctttca
     6661 gcagggcagc aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg ttttgctcaa
     6721 gaacataaat acattttgga ccataaggca taactgcctg cgcctggtcg gtattttgta
     6781 caatggcata cacctgttgg ccccattgct gagcgccgcc aaacagttca gcataacgtt
     6841 caggattatc gctaaagacc cagacgctgt ttaattgact catcgtttct tctccgtagg
     6901 cttcagttca gggctttctt cagatgttcc gccagctcgg caatggcctc cggcgaatcg
     6961 ttatcgataa tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc gaccagttca
     7021 gcaagtggcg cgctctggct ccagtcaata ccacttgcct gccactgatt taccggtttt
     7081 ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg gcacgttaat atcggaggtg
     7141 acgcagagca cggctggaac agagagttca ataacttcaa catcatcttc aagcgtgcgt
     7201 tcaatcacca gtgtattgcc ctgacgctga atagcactca ctgcattaat caccggaagt
     7261 tgcagaattt ctccgaccag caagccagcc tgctgggcat aaaggtcgcc ggaaccttca
     7321 ccaaagatca gtaaatcgaa gccgatcttt tcaactgctg ctgccagcgc ttttgcggta
     7381 tcgagaggca gtgcatgttc aagttgcgca tcctgcacca aatacaggct gtgcggcccg
     7441 cgggatagca cgtctttgcg cactttcgag ttctgcaaca atgagccgcc aacggtcagc
     7501 gcggctatct catcgtcatc tgttgcaagc tggcttgcag cttcaatggc attgagatcg
     7561 aactggctga ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg agtgacaaca
     7621 atgtcctgtt cttcaggcac cagcttaaag caggttatta ttttcatgat ttctcctgtg
     7681 agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata
     7741 gcggtgcggg gttttcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa
     7801 tgcgatcgct aaaacaaaag tgcaatttgc ggcaatatag ttattccatg accgtacaat
     7861 cttgcattca gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa
     7921 tacacgccgt attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg
     7981 tcatgtatca acgctgtttt gataatgcca gtgaaacgct gtttgtcgcc ggtaaaacgc
     8041 cacggctttc acgttttgca attagcgatg atccaaaatg ggagtctgga catcacgttc
     8101 atgacaatga aaccgagctg atttacgtca agaaaggggt agcaaggttt accatcgatt
     8161 cttcgttata tgtcgcacat gcggatgaca ttgtggtgat agaacgcggc aggctgcatg
     8221 cggtggcctc tgacgttaac gatccggcaa cgacgtgtac ctgtgcgctg tacggctttc
     8281 agtttcaggg ggctgaggaa aatcagctac tgcaaccgca ttcttgtccg gtaattgccg
     8341 cggggcaggg aaaagaagtc ataaaaacct tatttaatga gctaagtgtg attttgccgc
     8401 aaagtaaaaa tagccaaaca tcttcgttat aggatgcatt tgcctatacg ttagccattc
     8461 tttactacga aaattttaaa aatg
//
LOCUS       DAZFWP020000193         8276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFWP020000193 DAZFWP020000000
VERSION     DAZFWP020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.194"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            191..961
                     /gene="yjjP"
                     /locus_tag="ACHK0Z_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             191..961
                     /gene="yjjP"
                     /locus_tag="ACHK0Z_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU4504324.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            952..1425
                     /gene="yjjB"
                     /locus_tag="ACHK0Z_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             952..1425
                     /gene="yjjB"
                     /locus_tag="ACHK0Z_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU4504325.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1532..2071
                     /gene="dnaT"
                     /locus_tag="ACHK0Z_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             1532..2071
                     /gene="dnaT"
                     /locus_tag="ACHK0Z_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU4504326.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2074..2811
                     /gene="dnaC"
                     /locus_tag="ACHK0Z_RS22120"
                     /old_locus_tag="pgaptmp_004424"
     CDS             2074..2811
                     /gene="dnaC"
                     /locus_tag="ACHK0Z_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU4504327.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2860..3354
                     /gene="yjjA"
                     /locus_tag="ACHK0Z_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             2860..3354
                     /gene="yjjA"
                     /locus_tag="ACHK0Z_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU4504328.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3608..5899
                     /gene="opgB"
                     /locus_tag="ACHK0Z_RS22130"
                     /old_locus_tag="pgaptmp_004426"
     CDS             3608..5899
                     /gene="opgB"
                     /locus_tag="ACHK0Z_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU4504329.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6039..7061)
                     /gene="lgoD"
                     /locus_tag="ACHK0Z_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             complement(6039..7061)
                     /gene="lgoD"
                     /locus_tag="ACHK0Z_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU4504330.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYQFATLAETYERDVINNRELI
                     KGVITF"
     gene            7200..8114
                     /gene="lgoR"
                     /locus_tag="ACHK0Z_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             7200..8114
                     /gene="lgoR"
                     /locus_tag="ACHK0Z_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU4504331.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         2095 a         2152 c         2010 g         2019 t
ORIGIN      
        1 gataatattc atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc
       61 ttctcccgat aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc
      121 gtgcttcatg atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa
      181 agggcgagtt atgcaaactg agcaacagcg agccgtaaca cggctttgta tccagtgtgg
      241 attatttctt ctgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact
      301 gggtcgggcg ctgggaatgg acagcgttga aagttctatc tcttcgaacg ccatagtgct
      361 gacaaccatt aaagatgggc aatgcctgac atcgacacgt aaaaatcacg atcgcggcat
      421 taatatgcat gtggtgactg aagtccagca cattgtgatt ctggcggagc accatctgct
      481 ggattacaaa ggcgtagaga aacgatttag ccaaattcag ccattacgtt acccaagatg
      541 gctggtagcc ttaatggttg gcctttcctg cgcctgtttc tgtaaactca ataaaggtgg
      601 ctgggatggt gccgtcatca ccttctttgc cagtacggcc gcgatgtata tccgccagct
      661 actggcacaa cgtcatcttc atccacagat caacttttgc ctcaccgctt tcgccgccac
      721 cactatttcc ggtttgcttt tgcaacttcc caccttcagc aatacaccca ccattgcgat
      781 ggccgccagc gttctgctgc tggtgccggg atttccgttg ataaatgccg tcgccgatat
      841 gtttaaaggc cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact
      901 ggctacctgc gtcggcgtgg tgatggcact gacgatttgg gggctacgcg gatgggtgtg
      961 atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg ccgccattcc agcggtcggc
     1021 tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct ggtgtgcgct gcttggcgcg
     1081 attggtcatg gttcacgaat gatcttaatg accagcgggc tgaatattga gtggtcaacc
     1141 tttatggctt ctatgctggt cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg
     1201 catccgaaag tgtttaccgt ggcggccgtt atccctatgt tcccgggcat atcggcttat
     1261 accgcaatga tttcggcggt aaaaatcagc cagttaggtt acagcgaacc gttgatgatt
     1321 accctgttaa ccaactttct tacagcttca tcgattgttg gtgcgttatc catcggtctt
     1381 tccattcctg gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa
     1441 gcacacggat gagagacagc ctcctctcct ccgtgtgtta ctataaaagt tatctccctt
     1501 ctcgttcatc gttccatatt tgagaaacag tatgtcttcc agagttttga ccccggacgt
     1561 cgttggtatt gacgccctgg tacacgatca ccaaaccgtt ctggcaaaag ctgaaggcgg
     1621 tgtggttgcc gtatttgcta acaatgcccc ggcgttttat gccgtcacgc ctgcacgcct
     1681 ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg ggaagcgatg tcgctctgga
     1741 cgatcaactc tatcaggaac cgcaagccgc tcccgttgct gtccccatgg ggaaattcgc
     1801 catgtatccg gactggcaac ccgatgccga ctttatccgc ctggcggcgc tatggggcgt
     1861 ggcgctaaga gagccggtga ccaccgaaga actggcctca ttcattgcct actggcaggc
     1921 ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa ctggcgcgca gcctgcaaat
     1981 cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg aatacggtca gcgaacctga
     2041 cagccaaatt ccaccaggat tcagagggta acgatgaaaa acgttggcga cctgatgcaa
     2101 cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat tcaaaacggg tgaagaacta
     2161 ctggcctggc agaaagaaca aggagcgatc cgctccgccg ctctcgaacg tgaaaatcgg
     2221 gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc gtccactgca tcagaactgc
     2281 tcctttgaga actatcgcgt tgagtgtgaa gggcagatga atgcgttaag caaagcgcgc
     2341 cagtatgtcg aagagtttga cggcaacatc gccagcttca tcttttctgg taagccagga
     2401 accggcaaaa accatctggc ggcggcaatc tgcaacgagc tgctgctacg cggtaaatcc
     2461 gtattgatca tcaccgtggc cgatattatg tcggcgatga aagatacctt caggaatagc
     2521 ggtaccagcg aagaacaact gcttaacgat ctgagcaacg ttgatctgct ggtgatcgat
     2581 gagatcggcg tgcagaccga atcgaaatac gaaaaagtga tcatcaacca gatcgtcgat
     2641 cgccgctctt cttccaaacg cccaaccggg atgctgacca acagtaatat ggaagagatg
     2701 accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc tgggtaacag tttgtgggtg
     2761 atcttcaact gggatagcta ccgtagccgt gtaacaggta aagagtatta atccccgttt
     2821 ctggcggtat actgcgacac aattccacct tgagtaatga tgatgaaaac tgttaaacat
     2881 cttctgtgct gtgccattgc cgccagcgca ttaatttcca ccggggtgca tgctgcgtcc
     2941 tggaaagatg cgctctccag cgccgccagc gaacttggca accaaaacag tacgacacag
     3001 gaaggcggtt ggtcgctcgc gtcattaact aacttgctta gcagcggaaa ccaggcctta
     3061 agcgcagata acatgaacaa tgccgcaggc attctgcaat actgcgcgaa gcaaaagctg
     3121 gcttcggtaa ccgatgccga aaacatcaag aaccagatgc tggaaaagct gggcctgaac
     3181 agtgaagagc aaaaagaaga caccaactat ctggacggca ttcagggttt gctgaaaacc
     3241 aaagatggtc agcaactcaa tctggataac atcggaacga ctccgctggc agaaaaggtg
     3301 aaaaccaaag cctgcgatct ggtgttaaag caggggctga acttcatttc ctgaaccgcg
     3361 aaacgccgcg ttttagctgt tacccggctg acgcggctct ctcctgccct tttccctgct
     3421 cctggacggt tttccccctg ttacacggga agcgaccgcg atcaaaaaag tctgtttata
     3481 aactaattcc taacaacatc gcattttttt gacactagaa ttgcagcaat atggcgacgc
     3541 cattacattg tgcttctcaa aaagcttaaa cgttggctaa acggcccggt ttagtgagga
     3601 tcgcccgttg tcagaactac tctctttcgc cctttttctc gcctctgtgc tgatttacgc
     3661 atggaaagcg ggacgtaaca cctggtggtt tgcagccaca ttaacggtgc tggggctatt
     3721 tgtcgtttta aatatcaccc tgtttgccag cgactatttt actggcgatg gtattaacga
     3781 cgcggttctc tataccttaa ccaacagcct gaccggtgct ggcgtcagca aatacatcct
     3841 gccgggtatc ggcattgtgc tggggctgac agcggtgttc ggtgcgctgg gctggatcct
     3901 gcgccgtcgt cgccatcatc cgcaccattt tggttacagc ctgctggcgc tcttactggc
     3961 gctgggttcg gtggacgcca gcccagcatt tcgtcagata acggaactgg tgaaatccca
     4021 gtcacgcgat ggcgacccgg actttgcggc ttattataaa gagccgtcga aaactatccc
     4081 tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt ctcgagcgga cctattttga
     4141 taacgaagct ttcccggatc tcacgcctga actgggcgcg ttgaaaaatg aaggcctgga
     4201 tttcagccac acgcagcagc tgccaggaac ggattacacg attgcgggca tggtggcttc
     4261 tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac gcctccgcct ctgtctccag
     4321 cttcttcccg cagaacatct gtctgggcga tatcctgaaa aactcgggtt atcagaacta
     4381 tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat gtgttcctga agtcgcatgg
     4441 cttcgaccac ttatacggct cagaagagct gaaaagcgtg gtggccgacc cgcactatcg
     4501 caacgactgg ggattctacg acgataccgt tctcgatgaa gcgtggaaaa agtttgaaga
     4561 gctttcccgc tcaggtcagc gattctcact gtttaccctg acagtcgata cccatcaccc
     4621 ggatggtttt atctcgcgta cctgtaaccg caaaaaatat gattttgacg gtaagccgaa
     4681 ccagtcattc agcgcggtaa gttgcagcca ggagaatatc gcggcgttta tcaacaaaat
     4741 caaagcgtca ccgtggttta aagataccgt catcgtcgtc tcttctgacc atttagcgat
     4801 gaacaacacg gcgtggaaat accttaataa gcaggatcgc aataacctgt tttttgtcat
     4861 tcgtggcgac aagccgcagc aagagacgct ggcagtgaag cgtaacacga tggataacgg
     4921 cgcgacggtg ctggacattc tcggtggcga taactatctc ggacttggtc gtagcagttt
     4981 atccgggcag tcaatgtcgg aaatcttcct caatatcaaa gagaaaacat tggcgtggaa
     5041 gccggatatc atccgcctgt ggaaattccc taaagagatg aaagagttca ccatcgacca
     5101 gcagaaaaac atgattgcct tctcgggtag ccatttccgt ttgccgctgc tgttgcgagt
     5161 ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac tcagcaccgc tgcgtttcca
     5221 gctggccgat ttcgctccac gcgacaattt cgtctgggtt gaccgttgct ataagatggc
     5281 acaactctgg gctccggaac tggcactctc caccgactgg tgtgtctcgc aagggcagct
     5341 tggcggtcag caaattgttc agcatgttga caaaacaaca tggcagggca aaacggcatt
     5401 taaagatacg gtcatcgaca tggcgcgtta taaaggcaat gtcgatacgc tgaaaattgt
     5461 tgataacgat attcgctaca aagccgacag tttcatcttc aacgtcgccg gtgcgccaga
     5521 agaggtgaaa cagtttagtg ggatttcccg tccagagtcg tggggccgct ggtccaacgc
     5581 gcagctgggc gatgaagtaa aaatcgagta caagcatccg ctgccgaaga aatttgacct
     5641 ggtgattacc gccaaagcat acggcaataa cgccagccgt cctattccgg tacgcgtagg
     5701 caatgaagaa caaactcttg tgctgggcaa tgaagtgacc accaccacac tgcatttcga
     5761 taacccaacc gatgccgaca cactggtaat tgtgccgccg gaacctgtct caaccaacga
     5821 agggaatatc ctcggacact cgccgcgtaa gctcgggatc ggcatggtgg aaattaaagt
     5881 tgtggaacgt gaagggtaat ttctgactga tgtgtaggtc gcataaggtg cgacctacac
     5941 atcttctccg gcctacaaaa catcattgag aaacagaaaa aaaacagcgc ccaacacttc
     6001 cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta
     6061 atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaactga
     6121 tagcgatggg ttaacatcat gtcggcagtg atttttcctt ccgccatcag tcgaccgact
     6181 ttagcaaaat cttccggcgt ggcgttgcgg ctgcccatca tcgtcgtttc ttttttatgg
     6241 aattccggat cggagaactg caactcacct ttaaacaggc cgacaaatac caccgtgccg
     6301 ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc
     6361 actttctgcg ccagcgaacc accaaactgc gcccgtagct gcgcgtcaaa atcttcggct
     6421 gacgggtcca gtaaaggtaa ttccagacgc gttgccacat gttcacggcg cgccggactg
     6481 gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccaaaccg
     6541 attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc
     6601 gcatgagcgc taatagcgaa aggttcaatc aatgcggccg cctgcgggtc aataccgtct
     6661 gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc
     6721 acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat
     6781 tgctgacagg caacataagg gatcacagca acttgctgac catttttaag atcagcaata
     6841 tttttaccca gcccaacaat ctccccacat atttcatggc ctaaaacacg tggataacta
     6901 aaaaatggtt gatttccacc ccaggcatga atatcggtac cgcaaatccc gacagactta
     6961 atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat
     7021 tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt
     7081 tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg
     7141 aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata
     7201 tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc
     7261 ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca
     7321 gccgcacaac agtgcgtcat attctcagcc acttacgcga atgcggcgtc ttaacgcagg
     7381 ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta
     7441 ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg atgataaacc
     7501 agcgtcagtt acgccctggg gaaacttttt ctgaactgca actggctcgg gccgcgggtg
     7561 tcagcccggt ggttgtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa
     7621 gcgaaaaacg cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt
     7681 ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc
     7741 atgatccccg ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca
     7801 acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc
     7861 tttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca gtcatctttc
     7921 attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg
     7981 agcatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc acccttgcgc
     8041 tccgtaacca tttgaattca gctaaacaat ccatgattcg atccattaat gagaacacgc
     8101 gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca cggttttatc
     8161 aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatt
     8221 tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc aacctt
//
LOCUS       DAZFWP020000194         8233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFWP020000194 DAZFWP020000000
VERSION     DAZFWP020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.195"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            88..585
                     /gene="ftnA"
                     /locus_tag="ACHK0Z_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     CDS             88..585
                     /gene="ftnA"
                     /locus_tag="ACHK0Z_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU4504332.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(623..862)
                     /gene="yecH"
                     /locus_tag="ACHK0Z_RS22150"
                     /old_locus_tag="pgaptmp_004430"
     CDS             complement(623..862)
                     /gene="yecH"
                     /locus_tag="ACHK0Z_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU4504333.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1053..2264
                     /gene="tyrP"
                     /locus_tag="ACHK0Z_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             1053..2264
                     /gene="tyrP"
                     /locus_tag="ACHK0Z_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU4504334.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2326..2991)
                     /gene="yecA"
                     /locus_tag="ACHK0Z_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             complement(2326..2991)
                     /gene="yecA"
                     /locus_tag="ACHK0Z_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU4504335.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3187..3273)
                     /locus_tag="ACHK0Z_RS22165"
                     /old_locus_tag="pgaptmp_004433"
     tRNA            complement(3187..3273)
                     /locus_tag="ACHK0Z_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3237..3239),aa:Leu,seq:taa)
     gene            complement(3286..3359)
                     /locus_tag="ACHK0Z_RS22170"
                     /old_locus_tag="pgaptmp_004434"
     tRNA            complement(3286..3359)
                     /locus_tag="ACHK0Z_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3325..3327),aa:Cys,seq:gca)
     gene            complement(3414..3489)
                     /locus_tag="ACHK0Z_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     tRNA            complement(3414..3489)
                     /locus_tag="ACHK0Z_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3454..3456),aa:Gly,seq:gcc)
     gene            complement(3641..4189)
                     /gene="pgsA"
                     /locus_tag="ACHK0Z_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             complement(3641..4189)
                     /gene="pgsA"
                     /locus_tag="ACHK0Z_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU4504336.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSASRADLLDQ"
     gene            complement(4246..6078)
                     /gene="uvrC"
                     /locus_tag="ACHK0Z_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     CDS             complement(4246..6078)
                     /gene="uvrC"
                     /locus_tag="ACHK0Z_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU4504337.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6075..6731)
                     /gene="uvrY"
                     /locus_tag="ACHK0Z_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     CDS             complement(6075..6731)
                     /gene="uvrY"
                     /locus_tag="ACHK0Z_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU4504338.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6873..6944
                     /locus_tag="ACHK0Z_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /pseudo
     CDS             6873..6944
                     /locus_tag="ACHK0Z_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7027..7203
                     /gene="yecU"
                     /locus_tag="ACHK0Z_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             7027..7203
                     /gene="yecU"
                     /locus_tag="ACHK0Z_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU4504339.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7190..7414
                     /gene="yecF"
                     /locus_tag="ACHK0Z_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             7190..7414
                     /gene="yecF"
                     /locus_tag="ACHK0Z_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU4504340.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7481..8203)
                     /gene="sdiA"
                     /locus_tag="ACHK0Z_RS22210"
                     /old_locus_tag="pgaptmp_004442"
     CDS             complement(7481..8203)
                     /gene="sdiA"
                     /locus_tag="ACHK0Z_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU4504341.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2049 a         2098 c         1923 g         2163 t
ORIGIN      
        1 aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac gccacagcaa
       61 caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat
      121 gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc
      181 agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg
      241 acgcatatgc agcgtctgtt tgattacctg actgataccg gcaatttacc gcgtattaat
      301 accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat
      361 aaacacgaac aattaatcac gcagaaaatt aacgaactgg ctcatgctgc aatgaccaat
      421 caggactacc caacatttaa tttcctgcaa tggtatgttt ctgagcagca tgaagaagag
      481 aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg
      541 tattttatcg acaaagaact ctctaccctc gacacacaaa actaatgctc tcaggcggca
      601 ggttttctgc cgccgtttca aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac
      661 cttcttctga aggtataaat ttgccttttg ctgccagaaa cgctaccagc tctcccgcag
      721 tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc gctttaattg
      781 ctgcttccag actggcatgc gtatattgct cgcctgattc aatcatcata tttaacactt
      841 catgaccgtg aatagagtcc atcgtccctc ctcaaaaaaa gcctagcgta gcgattgccg
      901 cttatgaaga ctttgcgcca gcgcaggact gaatgctttt tattgtacat ttatatttac
      961 accatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat
     1021 cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaaccctg ggaagtgttt
     1081 ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggccg
     1141 gtgttggttt tagcgttacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca
     1201 cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt cttggcacgc
     1261 tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct
     1321 taatgtatgc tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca
     1381 tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg ttcacttttg
     1441 ttgccggtgg cgtggtttgt gttggaacat cgctggtcga tttatttaac cgttttctgt
     1501 tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg catattcaca
     1561 aagtgaatct tttaaccctg ccgttgcaac aggggctggc tctgtcagca atcccggtga
     1621 tttttacctc atttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca
     1681 acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc gatccccctg gtggcatata
     1741 ttttctggca ggtggcaacg cttggcagca ttgattcaac aacctttatg ggattgctgg
     1801 ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc
     1861 atgttgagct ggcagtgcat ttatttgctg atttagccct cgccacgtca tttctcggcg
     1921 ttgcgttagg cttatttgat tatctggctg atttatttca gcgttcaaat accgttggtg
     1981 gacggttgca aactggtgca attacctttc tgccgccgtt ggcgtttgca ctgttttatc
     2041 cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga
     2101 ttatcccttc gctgttgacc tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg
     2161 tgaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg
     2221 tgcaattttt gattgcggca gggttgttac cagaagtggg gtgatcagat agcctcaaat
     2281 tccttattgg gtgccagaat taacgctgac acccaatttg acctcttaat gcaggcagca
     2341 ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc
     2401 tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag
     2461 atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatttt
     2521 ctctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca gcgctggttt
     2581 taacgaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat agccaaagca
     2641 ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc
     2701 aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccag
     2761 attcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc
     2821 ccacacggca accagccact gttccggttc aatctcttgc ggagaactca acaccgccgt
     2881 caataaaccg tccagctccg ccacatcaag gatggcgtgg tcagtgttgt atttggtcag
     2941 aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc
     3001 cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt
     3061 ttgagtggct ttgctggtga ttaaaaatta gggagggtgt aacgacaagt tgcaggcaca
     3121 aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt attcttatct
     3181 ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga gggattttaa
     3241 atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg
     3301 gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt taacgcgcca
     3361 aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta aactggagcg
     3421 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
     3481 tattcccgca ttcatcaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca
     3541 gtgccgccgt tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt
     3601 caccactttt gatcgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg
     3661 cacgcgaagc gctcaaatat tgcaacattg accacagagt cagtaccgca gccacaaaga
     3721 aaagtgcaat accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg
     3781 ccaccatctg ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt
     3841 tacccaactc cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg
     3901 ttgccgccgg tagggtcacc caccagctgt gataatgctc ggttaccagc accatggcga
     3961 tagccacgag aactttatct gctacagggt caaggaaagc accaaaccgg gtactctggt
     4021 tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga
     4081 gcgcggcggc aaacggcgac caggtgacag gcagataaaa gaccaataca aagaatggga
     4141 taaggatgac acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta
     4201 actatctgtt gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga
     4261 ccagaagatc ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac
     4321 gctggcgtta cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt
     4381 tggcccgacg ccttcaatgg tttccaggga gctggtattt ttgaccttcg cccgtttttt
     4441 acggtgcccg ccaatcgcgt gatcgtgtga ttcatcgcga atatgctgga taacatgcag
     4501 cgcgggagaa tctggcggca aactgaatcc ctcaccttcc ggctcaaaga acagtgtttc
     4561 cagcccagcc ttacgatcta ctcctttggc aacgccaagt agcagtggat gatttttatc
     4621 ccatgagaca tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc
     4681 gtcgataagg atcacatccg ggatcttact gtcgtcaatg gctttaccat aacgccgacg
     4741 cagcacctga ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa tgttatagcg
     4801 ccgatactcc gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg
     4861 ttcgcccatg gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa
     4921 cacgctggca agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt
     4981 taaggccgtc gccgcattgg tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg
     5041 tttggtttga acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt
     5101 tttatcgcta agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc
     5161 ttgtaaatag aactggccta cgaaggtttc taccacctcg ctcagttccg taccgccagg
     5221 cactttcggg aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg
     5281 gacacaagcc atgcccgcat cgaacgccac accaataacg tcgaggtcgt cgccggtatt
     5341 ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc
     5401 agcttcttca aactccagat tctggctggc agtttccata cgactaatga gttgcgtaag
     5461 cacctgatca tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc
     5521 ttcttcactc accagtcctt caacgcacgg tcccagacag cgccctatct ggtattgcag
     5581 acacggacgc gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt
     5641 ttgcagtagc gccagtgttt cacgtacggc atagccattc gggaacgggc cgaaatattc
     5701 acctttggca tgcttcgcac cacgatgcat cgccagacgc gggtgggtat caccactcag
     5761 gaagataaaa ggatatgatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag
     5821 tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct gtgtgagtaa ccgttacatc
     5881 aatttgctgg atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa
     5941 atagctggaa agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc
     6001 agcatcgtac atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc
     6061 gtcaaactga tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt
     6121 gccaggtgag tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg
     6181 tagctgttca ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg
     6241 cccttggtga tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt
     6301 tctgtttttt ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg
     6361 taacgctgcc ctgaatagac agaacgaatc gcactcacaa cttcctgcgg agccgcgcct
     6421 ttgctgaggt agcccgcagc accggcctgc atgactttcg ctggtaaagg gttttctgta
     6481 tggacggtaa gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc
     6541 tcaagaccgc caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc
     6601 cggcaccact taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt
     6661 atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga
     6721 acgttgatca aaggaatatc tccagaaata gggataacgc tactgatagt tagtcattcg
     6781 tatattagcg gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc
     6841 tctcgttttt gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac
     6901 cggatcgttt cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa
     6961 aaatgaaaat cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa
     7021 acattcatga taaaaatatt tattggtcat tatattaacg tattttatag cactgccgat
     7081 atcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata ctccgccgcc
     7141 ttaacatttt tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa tgagtacgcc
     7201 tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct gcctgaaagc
     7261 gatgctgacg ctgatgctgc aggcgatggg acaagctgac gcgggccgcg tgatgttaaa
     7321 gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat tttccaaaac
     7381 ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgag agcgtgcctt
     7441 tcagccggtt tttgcatctg gcacgcagaa agaaaagcga tcaaattaag ccagtagcgg
     7501 ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat ttttttctgc atgtttttct
     7561 gatggaaatt gaccgtattc tcagagattg acaaaatcat cgctatctct gctgatgtct
     7621 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
     7681 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
     7741 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
     7801 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
     7861 aaccatgtgc gcgcgcggct tcccataacg gctgtgcttc gctgaataag tcatcattcc
     7921 acattaaatg gccctgacta aagttttcag ggttcagcac cggatcaatt gcgagaaagt
     7981 tttttgcctg ataataacta acccacgcct cagggtaatt ggtgtagaaa gccactttag
     8041 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
     8101 gagcctgaag ctctatttca tggtagacct cttctgcggc ctccatcctc tgaaaacgca
     8161 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
     8221 ctgagagtga gta
//
LOCUS       DAZFWP020000195         8142 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFWP020000195 DAZFWP020000000
VERSION     DAZFWP020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.196"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..114)
                     /locus_tag="ACHK0Z_RS22215"
                     /old_locus_tag="pgaptmp_004443"
     CDS             complement(<1..114)
                     /locus_tag="ACHK0Z_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504342.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRE"
     gene            complement(330..710)
                     /locus_tag="ACHK0Z_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             complement(330..710)
                     /locus_tag="ACHK0Z_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4504343.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(729..1718)
                     /locus_tag="ACHK0Z_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             complement(729..1718)
                     /locus_tag="ACHK0Z_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4504344.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(1770..2027)
                     /locus_tag="ACHK0Z_RS22230"
                     /old_locus_tag="pgaptmp_004446"
     CDS             complement(1770..2027)
                     /locus_tag="ACHK0Z_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504345.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(2024..3424)
                     /locus_tag="ACHK0Z_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             complement(2024..3424)
                     /locus_tag="ACHK0Z_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4504346.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(3421..4299)
                     /locus_tag="ACHK0Z_RS22240"
                     /old_locus_tag="pgaptmp_004448"
     CDS             complement(3421..4299)
                     /locus_tag="ACHK0Z_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4504347.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(4310..5218)
                     /locus_tag="ACHK0Z_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             complement(4310..5218)
                     /locus_tag="ACHK0Z_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4504348.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(5205..5438)
                     /locus_tag="ACHK0Z_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             complement(5205..5438)
                     /locus_tag="ACHK0Z_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504349.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(5435..6097)
                     /locus_tag="ACHK0Z_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             complement(5435..6097)
                     /locus_tag="ACHK0Z_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGU4504350.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            complement(6206..6913)
                     /locus_tag="ACHK0Z_RS22260"
                     /old_locus_tag="pgaptmp_004452"
     CDS             complement(6206..6913)
                     /locus_tag="ACHK0Z_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4504351.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            complement(6995..7228)
                     /locus_tag="ACHK0Z_RS22265"
                     /old_locus_tag="pgaptmp_004453"
     CDS             complement(6995..7228)
                     /locus_tag="ACHK0Z_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4504352.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            7385..8074
                     /locus_tag="ACHK0Z_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             7385..8074
                     /locus_tag="ACHK0Z_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4504353.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQSKVI
                     SSVPFNINRFG"
BASE COUNT         2007 a         2182 c         1888 g         2065 t
ORIGIN      
        1 ttcgcgtgaa aggtacgtat tctgtgcaat ctccccgact gtcgccggtg cggtggcact
       61 taattcatta aacaccactc tggcggtttc tgtcatatcc tgctgtttca gcatgtcttt
      121 ttcccttttc cggttaacgt gacacaccaa taactcttgt cgaaaaagcc agcaagctga
      181 aagaccggta ttcaccgcca ccagcgcgtt taacgtactg gtccgatttc aggcataaaa
      241 aacccgcctg acggcggatt taagctgtgt ggcaaagtaa ccactcttaa catactgaca
      301 tactttttgc ggaccgcact aatcattttt tactttttta gcagccagtc gtccatctcc
      361 agtcttaccc ccagcacaga caaacatccg tcaataaacc cttcggctat ctgcatctca
      421 attcgtattg ctttttcgct tttctttctc gtcctggcta tctgtctttt tgatattcgc
      481 aacaaataat gagcaatgag aagcgaatac tcctcaggtt ttttctgctt cagacgtgca
      541 agacagtttt caatgataag tccgtcatca tcgcagcagg ctggacgtgg tttagtggta
      601 gatggtaaaa ggcctttgaa tccggcagcg atcggagaat agtccacccc ggtgttacca
      661 cttgccgccc atgcccccca gcgttcaaga accatctgaa tatcacgcat caactttctc
      721 cacaaaaatc aggacagcac accaatcgcc agtgcgcgat cgataaaacg aaatatcagc
      781 tccagttggg aaccatactt ctcttcaaat gccacggtat ccgcatgcag ttcgtcatgg
      841 tgttttctgc acaaaggcaa cacaaaaagg tcatgcgctt ttgtacccat tccaccctga
      901 ccatggccaa tcaggtgatg tggatcgtcg gctggcttac cacaacacgc gcaaggctgt
      961 gtcttaaccc agcgcgtgta cttttcatta acccagcgac gacgtttggg gcgtaacata
     1021 aaagactccg gcgactccgg atccactttc agcgccagca cctttttcgc tttatcctgg
     1081 ataatgctgg tggcaggaac cgaaggcaca aggtcactct cccgggtgac agacggcaaa
     1141 acaggcttcg gtaatctcag ggccttacgg gctgcacttt ccggtaaggc atccgccagg
     1201 tcattacgaa ccagccacca gcacagttca ggcattgtca caacgtgact gtcatcaaaa
     1261 ccgagatcac gacggactac ggacaacacc cagcgggcac agttatccgt tgccattgac
     1321 tccagacgtt ccgtgaactg atcgcgaagc aggttatcgc agtgccagca cagacggatt
     1381 gcgcccggcg cgtgtcgcat tgtggtcatg ttctcgctgt gccatccgga atgaggccac
     1441 tggcagcctt tttcacgaag taaccagctc tcaaggcatt ccacgccacc agcacgacgg
     1501 atcaccgcct catggcggaa cacggcccga acggcaggat catccgccag cggttgtgat
     1561 gctgccggaa cggcaccact ggcaaaagat gaataacgtt ccggctcagg ctccagcagg
     1621 acacgcccct gcataaacag gggcatcagc tctgaaccgg gtctgaacaa gacgatcccc
     1681 atacgcgggg caatttcagg ggtcagcagt gctctcacgg tcacctcagc gaacggtatt
     1741 gcatgaacgc aggagaaaaa attcagccat cacgcagtaa actctttcac cagcgtttca
     1801 aactggctta cctggccttc cagttccgcc acgcaatcca ccagctcatc caccgccttt
     1861 tgtgtgcgat gttttgcctg cagcagatca cgaagcgccg gagtaagctg cttgcggagc
     1921 gtatcttttg ccacgctcgt tttttccatc tgttcagcac aacgaagcat ctcctgcgcc
     1981 tgccgacgaa gttgttccgg tgaaacagtg gtggttctgt tgttcaaaat aaacgctccg
     2041 ttttactacc cgacatgcgg ttattgctgt atctgcgcgg attgcccggc gtcatgggag
     2101 tggaaagaac ccgggcactc tcctggtcca caggcagaaa atgcccgtta tgaaaacgcc
     2161 ggtaaatggt acccagcgtg ccattacgct gtttcgtgat gttgatttca gctatgcctc
     2221 tcgcctgtgt ctccgggttg tacacctcat ccctgtaaag catcagaatg atgtctgcat
     2281 ccgcctctat ttcccctgag tttttcaggt ccgagttcat ggggcgttta ttgggtctgg
     2341 attccacgcc acgggagagc tggctcagag caatcagcgg aaaaccgccg gattttgcca
     2401 ggcttttaag tccctttgag atttccccca cagcaaggtc gtgacgcccc gtgctgcggg
     2461 ttttaatcag gccgaggtaa tcgaccacca ccagcgccgt ttccgggtgt ttcatccggt
     2521 ggtgcctcgt ggttgcacat atctcatcaa tggtcaggtt tgcctggtcc accatccaga
     2581 tattacgccc cgtcattcgt cccacgccct gtgagaaacg cgcccagtct tcatcttcaa
     2641 aacgggcaac agacttaaga cgggataccg gcattccccc ggcagcagac accatacgtt
     2701 caccaatctg aatgttcgcc atctccatgg tgaacagaag cacgccatgc ccctgctcag
     2761 tcaccttgtc gatgatatcc agcgcaagtt cggttttccc catcgaagga cgggccgcaa
     2821 tgaataccag gtcgcctggc tccataccgc ccgtttttgc gtccagttca tcaataccgg
     2881 tcatcagcgc cctggatttc tccagtccct gattgcggca ttcaacacgg tcgaccactt
     2941 ccggaaggac atcatcaatg tgaaccggct gaatgacgcc ctttccggtc gacagtgagg
     3001 ccatcatgtt ctgcgcatcc ttcagggcat cctcggctgc ttcacaggta tgcgcatccc
     3061 gtaaattctg taatgcttca gtcagtgttt tttctgcatc gcgcagtgcg gcattacgcc
     3121 gcaacgctgc gacatagtgc tccagtgaag acttcaccca ggttttgcgt ccggtgtcgg
     3181 taatcaccgg ggcaagttcc ggcatctcat tgcacagcag tacggggtca atgacgccgg
     3241 atacacgtgc ctgtctgcaa attcccgcgt aaatatcccg gtactgtcgt gcagaaaaaa
     3301 cgtccgccga caatgtggcc agaatatcca tcacttccgg atcagcccca cgcagaaaaa
     3361 acgcgccaat gacagcgcct tccaggtcat cgttacgcca tgccggggtg ttctggctgg
     3421 tcatgcggca acacctccga tacgagaacg gtagctgggc cagttaaacg acaaccagtt
     3481 gcgcccgcca ttggtgatcc tgtcggcaat ccggggactg atgaacgccc acaattcttc
     3541 cggtgaaagg ttgctgatca agatagttgg caaaataccc tcataccggg cattgataat
     3601 ttcctgcaaa atggccattt cagccgcact gccaaactga acgccgactt cgtcgatgat
     3661 cagcaaatcc agtgacgcat aatgctcaat gacgtcatcc gctgtttttt cactgtcatt
     3721 ccgccagcag tttttcacag cacgggtaag gcgcatcacg tcggtgatct ccacactggc
     3781 cagatagtta cggatgatgt gttttgccat tgataccgcc agatgatttt tcccggtacc
     3841 gcaactgccg gtcataacaa gactggtacc gttctccagc atatctggcc agttctccgc
     3901 atagcggcga caggccgcaa gatttctggc tgcgtcagga ttaacctcca gataattatc
     3961 aaactcgcag tcccgaaaac gcagagcaat tccggcgtta tcagtcagtt cttccgcctt
     4021 gagggacgac agctccatgg tcaaatcgtt ggcctcagct atcaagcagt cagggcagca
     4081 tgaaattttt tctctgtcct cgccattacg atcgctccac accagaatat gtgtgtggta
     4141 ttcgccatgt ttttcgcaac acccgcgccc ttcacgcatc cagcaggaac gataaggcca
     4201 tggtttttcg cccttctgag caaatgcaat ctctgcccgt aactcatcca tccgcgcctg
     4261 tagtcttgtt tgttgttcac gcaggttaaa cgtcatcatc gctgtcacct cagaatgtca
     4321 atttgtcact ggatttaccg aatttgtcag acatggctcc caggccagcc aggacatcga
     4381 cctgtcgctg tcgcccacct ccgggagctg ctggctgttg ccagaaatct tcgaagtgac
     4441 gatcgggtcc aaagaacgtc gacgcctgct tcacgaactg ggtgccggta tttcctgaga
     4501 cacgcaccca ggcagcatag cgtttcacac cgttgagcat ggtttctggt gtcacacctt
     4561 ccctgattcg ggctttccag gctttgaagg ctgctgactt ggaattacca ccagcacgtt
     4621 tgggatattc ctgccaggcc tgttcaaatt ccggtgaata ttcctgtcgg gcagaacgcg
     4681 ctggtgcaga cgcgtcagcg gatgcgccaa tagtgttttt actctctgta gtattctctg
     4741 aagtaatctc tgttgtattc tctgtaagat cgaaattggt tttcccttct ccgcggcgag
     4801 tggtttcccg tgtccgcggt gaaggctttc cctcctccgc gaaattgggt tttacagttt
     4861 cccgaaaacg ggtttcccca tttcgggaaa actgattgtt ttcattgata atttcattaa
     4921 ggcgctcaca atctatacgg tagaacattt tgtgctcaag acgcttgttg gtttcaacca
     4981 aaatgcctct ggacacaaga tgcttacgcg ctacagcctg ttgttcaaat gtaagtccag
     5041 tttcgtgttg tatctcttca cgcgttttat gtacgccttc cgctgcatgt gctttatcct
     5101 gccagtaaaa aatctgacca aagaaaataa cagcgtgcgg acttcccatg tatttaacga
     5161 gcccagggta ataagcaacc ggatgcccaa aatcgagcag aagatcagac ggacgcatag
     5221 ccacctccca gacgcttaaa cattttcccg gactgaaaag ccaccagcgg ataactcagg
     5281 gtatgagtac gtccctgaac ctggcagaca accatctggc tttctgtatt gaccaggcaa
     5341 acccgcagaa cgtggccgtt gctggtggtg aaccactgcc ccacacgggg gcaacggttg
     5401 tatcggtgat acagggaatt aacgacgcgg cgaatcatgg gcgcacctcc cattgattac
     5461 ggcggaaagc ggtgtgactg aggctgattt ctgcctcatg gaatgcttca atgcagctct
     5521 cgtagtaccg cattgtgcgt agacttaacc caagctgaag catcatcaga ccatcaaggg
     5581 tgatgtaata accacgcagg gagtcaccgt agatgtgata agtacccggt atgaaattgc
     5641 gggtaaaaaa ctcgcgtgag cagttcagat actcgatttt gtcgacgatg ttctggtgca
     5701 tgcgcttaaa gtggcaggca acatgcaggg agaaaataac ggccttgccg ttgacaactt
     5761 caattttcag gtatggggaa gttgggactg tagccatgat ggcagcctcc ttgagcagtg
     5821 aaaaacttcc accaccggag ctgcgaaact catgggtggt gaactgaaca gggttcgcag
     5881 taccggcgct caaggaaacc ggcgagcctt tcggctcccc tgcccagccc accataattc
     5941 tggcgtgcgc gagcatggac gataaaaaag acgctggcgc gtcgtatatc gcctcgagca
     6001 attccgggct gcgacccccg gcacccgctt tataaggtgc ggagacagtg taacgtcccg
     6061 aaattgcaga atcaatattt ggtcttgaaa tgatcatata gctgctgata tctttagaac
     6121 tgttcttgga tgtttcggag ccgttttatg cgaaacagct ccccgttatt gatgttgagt
     6181 gagccgggtt actcccggct ttttttcacc gctgccaacc aataacctga aataacccca
     6241 ttttcgggtg ataccagcga gtccctcgcg gttctgcttc ctccataacc cgataaaaag
     6301 cagccataaa cggttccaca gcaacaattg cgcgacgtga caacaatccg tccggcgtca
     6361 tgaactcatg tgtgtctgta ggaatttgat aggcgttcac cagattgcag catttatcat
     6421 ctgacaaacc ggtttttgct ttcagttggc gatatccggc atagccctca cgaatagtgc
     6481 cctttttaat ttgctcaact gtttcagcaa cgtggatgac tttttcttcc acctgagtga
     6541 tccgtttctg ttggcgaacg gcttcaagag ccatcgcggc aaccatttcg atttcgctca
     6601 ttggcttacg gatctgttct tccagttcac gccagcggtc taccaagcgg gcggtgaatt
     6661 cagggcaaat ctgcgcgaca acaatgatac tgtcgcgttt gccttgttca ccttcgaata
     6721 cgtaagcagc tacatttctt ttcaatccta agttgttgat tatttcgaaa ttcgccattg
     6781 gcgcacttcg gataactccc ttccccatga gtctttcaat gctgcgttta acatctttgt
     6841 gttggctgcc caccagctct gcaatctcaa cgctggtcat ggatgcttta tcgttaaaaa
     6901 ttgcggtgtt catctgattg ctccttgagg atgacatttc agaactcacg ccaaaacgta
     6961 ttcatcgtga attactgctt gtgacagtca gtctttaatc tctggtaata ccgtttgatt
     7021 ttcgtaacga atcaggaatt ccatcttcgg gataagggta tagatcaggt cttaacccat
     7081 gcggagtaac cttccattca accaattcac acacccgtaa aacaaaacga gaaggaacgg
     7141 agtttttaga aaaccacaga ttcaccgctt gtggcgtaac accgatgtat ctcgctatgg
     7201 cgttttgagg gatcaattta cgcaacatgt cgtaatcatt cagttttatc acagcgcagc
     7261 tccaacatta actttacaaa tcaagaatac atcaagaata aattaacatg caagtttcaa
     7321 aaggatcgaa tacactaaaa tcaagttaat atttatgtgt ataaagcttc gacaggaact
     7381 caccatgaag aatgtaaaaa gcacagaaaa tcggatagca atgatgctga aaacgaaagg
     7441 ctggagtcag gctgaactag cccgtaagct gggtgtaagc gcgcaatcag tacaatactg
     7501 gactacagga aagacgtttc ccagaagcga taaacttgca cagctatcag taattagtgg
     7561 ttatccacaa tcctggtttt tgggtgaaga tgcctcatcg acattctctt cagctgaaaa
     7621 acaccataca agagaagaca gcgttgtgtt caatgtactg gatgttgaat tcagttgcgg
     7681 cgacggaact catgtacggg gagatctaat tgatgtagta cgctcaatag aacttgatcc
     7741 tgaatatgcc cgccgcctgg tcggtaacag agcattcaag aacatagaaa tcggtaacgc
     7801 cagaggggac agcatggccc caacaatctc cccgggagac ttactgtttt tagataaaac
     7861 agtaacttac tttgacggcg atggcattta tgcattttgc tttgatggcg aatgttacgt
     7921 caaacgactt caaaaaattg gaagcaagat catggtctta tcagacaatc ccaactatca
     7981 gccatggagc atcgaaaaag agggaatggc gctgctctat atccagtcaa aagtaatctc
     8041 atctgtacca ttcaatatca acagatttgg ttagttattg attttaaatt aaattattgg
     8101 tcatccatca caaaaaaatc aagtttatca atttttaatt ga
//
LOCUS       DAZFWP020000196         8070 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFWP020000196 DAZFWP020000000
VERSION     DAZFWP020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.197"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            40..1254
                     /locus_tag="ACHK0Z_RS22275"
                     /old_locus_tag="pgaptmp_004455"
     CDS             40..1254
                     /locus_tag="ACHK0Z_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504354.1"
                     /translation="MPVRLNTIPDAESYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPHFWGLACGVPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVYLEPKTPERTAESSTALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGIWTAELDREQRTALLSL
                     NQPFTQEALMYELDAFLGRSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1311..4637
                     /locus_tag="ACHK0Z_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             1311..4637
                     /locus_tag="ACHK0Z_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HGU4504355.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKK
                     LRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQA
                     GRPLQSVGCLLPERCTPEQLTAMLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAER
                     GIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSQVWAGVTGDNAR
                     GRAVGFPWLRAALMSAVCVLAIWGAGMTTSFFANCALVQETGIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADSLRY
                     ENIPEGVQQSVFPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARHEEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFTGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELQAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            4630..6240
                     /gene="tssA"
                     /locus_tag="ACHK0Z_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             4630..6240
                     /gene="tssA"
                     /locus_tag="ACHK0Z_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4504356.1"
                     /translation="MSELLQKLTRSCFVDRDALDVARTQAALWQTWLLPVTANTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALSQLTVSFTAWPEDQHSPSLMLLINALESRLAQSGGMNAVVPQNSSSVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDDTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            6246..7664
                     /locus_tag="ACHK0Z_RS22290"
                     /old_locus_tag="pgaptmp_004458"
     CDS             6246..7664
                     /locus_tag="ACHK0Z_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4504357.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIGGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGGSEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSEQQTGKYFDKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLHNSPEEQKWVDVKVDRKQKTVDIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVNLTKNGVEFYWSRNGSRGGGIGENIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            7657..>8070
                     /locus_tag="ACHK0Z_RS22295"
                     /old_locus_tag="pgaptmp_004459"
     CDS             7657..>8070
                     /locus_tag="ACHK0Z_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504358.1"
                     /translation="MTRVVIALLFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTVNKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDSQDI"
BASE COUNT         1908 a         2109 c         2348 g         1705 t
ORIGIN      
        1 taacttctgt ccgataatgc aaaataaagg gatgtactga tgcctgtccg acttaatacc
       61 attcccgacg cagaatccta tcccgcaccg ccagcccgca ttaaatggct gtcagcctta
      121 ttactgatgc tgtctgttgg tgtggcaata acctcattat tcgccagcga tgaactggca
      181 aaaaacggcc cgcatttctg ggggctggcc tgcggtgtgc ctgcctttat ctggtcgctg
      241 gttgcttcag ttcgctggtt gttttttatc acgcagtaca ttcgggctga tgcctggaat
      301 aagcgccggg aagaggtgat tttgcaggaa acgcgacggg gacgacgggc gttgcagata
      361 cttagttttt ccgtacaaac cgcgctgaat ggtgattccg ttgcagaaac tacaacagca
      421 tttctggcgc gtcagcaggt gttgaatact tacgctgatt tgcgcggaga agatactgtg
      481 cgtcgcagtg tcattcccgt attaaccaat aaaccggtta ccgggcgttt atccgaaatt
      541 atttcccgcc tttttattga tatcagaccg caattgtttc tgctgccccc tgattttcat
      601 atcaacgtac tactggaaat tgatgcgccg ctttcagggg cctccgtgag aactatctgg
      661 caggacgaat ggcaaaaagc cggactcccg gaagcccgtc tgttttctgc cccggagccg
      721 gggctggccg ccgtggacga ctggctggac aactttattc aggaaaaggc cgtactgctg
      781 gtgatttccg tgtatctgga accgaagact ccggaacgga cggcagaatc ctccaccgcg
      841 ctgttgctgg caaaccggtt gacgcaaacc gcgctgacgc cgctggccct gctgcatcgt
      901 cctgaacgta tcacagatac agagatgatg gcgtcaggca ttgcacaggc gctggactgg
      961 atgccggtgc agccagatgc catatccggc atatggactg ccgaactgga cagggaacag
     1021 cgtaccgcgt tgttatccct taaccagccg tttactcagg aagccctgat gtacgagctg
     1081 gatgcctttc tggggcgctc cggaccggca gcgccgtggc tgtccgtcgc tgcggcaacg
     1141 ctggcggcaa tacaatcaca acacccccaa ctaacgctca gcggcgttca gggcgggcat
     1201 tattcatggg cgaccgtggt gtcgccattc gtttccccac aggaggcatc atagtggttc
     1261 cgggattaaa ccgtctccgg ccccgttctg tttcagggcc tgccatcgtc atgaccgtgg
     1321 ctgttgtggt tgcgctttgc acgtttattc accgtaaacc ggcattgctg ggcgttgaac
     1381 cgggtagctc acagcaaatg tactggatgg ctggcgtgat tgccgcaggc gtggtctttt
     1441 tgattatcat gggggtttat catctgctga accgccacgc tgccgtacaa aactttgaac
     1501 tccgcagaca acttgccgcc ggagatgact atcgtgcggt gacgacaaag aaagccgaca
     1561 gccgtcatcc ggcaatggtg gaactcgccg ccttcctgcg cgaccgctac ggtccgttct
     1621 ggcggcgtaa cgtccgcctg ctgctggtga ccggcgagcc tgaacaggca gaagccatcg
     1681 cgccggggct gaccgggcaa cactggctgg agggcgacca cacggtgctg atatatggcg
     1741 gcaggccatc agcggagcct gatgtcacac tgctgaccgc cttaaaaaaa ctgcgccgca
     1801 gccgtccgct ggacggcatc atctgggcgc tgacagaaga acagagccgc cagacagcgc
     1861 aactcgacaa aggctggcgc gaactgataa acggcggtaa gcgactcggt tttcaggccc
     1921 cactctattt gtggcaggtc tgtgacaacg gtgattatca ggccggacgc cccctgcaaa
     1981 gcgtcggctg cctgctgccg gaacgctgca ccccggaaca actgaccgcc atgctggaag
     2041 ctcacaccct gccgctgacg gaacagggca tgtcgcaact gctggctgat aaccgccatg
     2101 attttctgct gcgtctggcc catacccttg cagagcgggg tatcgctcac tggcaaagcg
     2161 tcctgaaacc gctgctggca ggcggcgcat tctcttccct gcgcctgcgt ggtctgatgt
     2221 tcagcccgcc gcttaccgcc gtaccggagg ccgcacctca tgcgtggctg ccgtcacagg
     2281 tctgggcggg cgtgaccggc gataacgcac gggggcgtgc agtgggcttt ccgtggctac
     2341 gcgccgcgct gatgtccgct gtctgcgtgc tggcaatatg gggggccgga atgacgacct
     2401 ctttcttcgc caactgcgct cttgttcagg aaaccggtat ccagacggcg cgggcgctcg
     2461 atacccgcct gccgctggca gaacaactgg tggcgctgca taccctgcag ggcgaactgg
     2521 aacgcctgca atatcgtatc cgcgaaggtg cgccgtggta tcagcgtttt ggccttgaac
     2581 gtaaccaaca actgctcgcc gccgcctttc ccggctatgc gcaggcggca aaccggctgg
     2641 tgcgcgacgt ggccgttgac catctgcaac agcaactgaa cgcctttgtc gccctgccgc
     2701 ccaacagtcc tcagcgtacc gccaccggtg aacaacgcta taagcagctt aaggcattgc
     2761 tgatgacttc ccgcccggaa aaggccgacg ctgccttttt cagtaccacg ctgatggcag
     2821 acagcctgcg ctacgagaat atcccggaag gtgtgcagca gagcgtgttt ccgtcactgc
     2881 tgaccttctg gatggcgaac ctgccggaac acccgcagtg gaaaacctcg ccgccaccgg
     2941 aactgaccgg cgcggtgcgt aaaatcctgc tgcgccagat tggtgtgcgt aatgccgaaa
     3001 acactctcta ccagaacgta ctgcaacagg tgtcccgcaa ctacgccgat atgacgctgg
     3061 cggacatgac cggggatacc ctcaccgaat ctcttttcag tacggaacag acggtgccgg
     3121 ggatgttcac ccgtcaggcg tgggaaggac aggtcaggga agccatcgag caggtggtga
     3181 cggcgcggca cgaggaaatc gactgggtgc tcagcgaccg ccagcaggat acctctgcgg
     3241 atatatcgcc ggatacgctg cgtaaccgtc tcacctcacg ctactttacc gacttcaccg
     3301 gaagctggct ggcgtttctc aacagcattc gctggaaaaa ggaagactcg ctctccggca
     3361 ttctcgacca gctgacactg atggccgatg cccgtcagtc gccgctgatt gcgctgaccg
     3421 ataccctcgc ctggcaggcg gcggcaggca gggaaaaccg tggcctgtca gactcgctgg
     3481 cgaaatcggc acaggaactg tttaacggca aggagaaaac gccgcagcaa tcccgtgaag
     3541 gcgacgtgcc tgtcgggccg ctggataaaa ccttcgctcc gctgctgcgg ttgctgggcg
     3601 ataaagccgg aggcggcgac agccagctga gtctgcagac ctacctcacc cgcgttaccc
     3661 gtgtgcgtct caaactgcaa caggtgacca acgcccccga cccgcaggaa atgacgcaac
     3721 agctggcaca gacggtactg cagggcaaaa ccgttgacct caccgacacc cgcgactacg
     3781 gacggttaat cgccgccagt ctgggcgagg aatggagtgg cttcggtcag gcgctgttcg
     3841 tccgcccggt ggagcagtcg tggcggcagg tgctgacgcc tgcggcggac agcctgaacc
     3901 gccagtggca gcgggcgatt gtcagccact ggaatcagga cttcgctggt cgctacccgt
     3961 tcaaagcctc acagaacgat gcctccctcc ccctgctggc gcagtatctg cgcgatgacg
     4021 ggcgcatcaa ccagtttatt gccgccaacc tttccggcgt tctgaaacgt gaaggccgct
     4081 actgggtggc ggacgccatg aacacgcagg ggctgacggt caacccggac tttatccgcg
     4141 ccctgaaccg cctgcgcgac gtggctgaca ccgcctttgc cagcggcgat gccggaatac
     4201 attttgaact gcaggcaaaa ccggcccgag acgtgatgaa gacacatctg gtgattgacg
     4261 ggcaggaact ggagtatttc aaccagaaag aacgctggca gcgtttcaac tggccggatg
     4321 aacagtggca acccggcgca tcgctgagct ggaccagtac gcaggcgatg gaacgcatac
     4381 tggcggatta ccgggggagc tggagcctta ttcgcctgct ggaacaggcg caggtgacac
     4441 cggtggacag cagcaccttt aaagtggtgt ggaaagcgca ggacggcctg ccgctgaatt
     4501 acctgctgcg ggttgaacag ggtaaagggc cgctggcgct gctggaactg aaaaacttcc
     4561 gcctgccggg acaggtattt ctgaccggca ggagtatgaa agatgcggaa gaatacgggg
     4621 aagacgccga tgagtgaact gttgcaaaag ctgacccgta gctgttttgt cgaccgggat
     4681 gcactggatg tggcgcgaac acaggcagcg ctgtggcaga catggctgct gccggtgacg
     4741 gcaaatacgc cggtcgggga agaccccggc taccacgacg atttcctgcg tatccgcgac
     4801 gagatgaaca aactttccgg cgcggatacg gacctcatct gccagcttgc cgaatccctg
     4861 ctgctgacgc aggcaaaaga cgtgcgcatc gccacttact acatctgggc gcgactgcac
     4921 cgcgacggtg agcgcggact ggctgagggg ctggcgctac tgaccggact ggtggaacgc
     4981 ttcggtacgc aactgctgcc ctcgcgaccg gccagccgga aaatggcgct ggagtggctg
     5041 gcaggggaaa agatgctcga cagtcttgcc cgctacccgg aagtggcaaa agaggatttt
     5101 gcgaacattg ttgctgccct cagtcagttg acggtttcct tcactgcatg gcccgaagac
     5161 cagcattccc cctccctgat gctgctgatt aacgcgctgg aatcacgtct ggcgcagtcg
     5221 ggcgggatga acgcggtggt gccgcaaaac agcagcagcg ttcctgcacc gtcatccccg
     5281 gtggatgcgc cacaggtaca gaccatcacc tccgggcgtg acctgctcga ccaggcgaaa
     5341 gtgctggccc gttatctgaa cgaacagccg cagggctggc tttcggcgca ccggctgatg
     5401 aaaaccctgc gctgggacac agtgcatgaa ctgccgccgg atgtggacgg caaaacccgg
     5461 ctggccccgc cgcgcaccga gtcacgcaac cagcttaagc ggctgtatgc gcagcagaac
     5521 tggacggaac tgctggagca ggccgacctg atgttcagca ccggcgtcag ccatttctgg
     5581 ctggatatcc agtggtatct gcatcaggcg ctgactaagg cgggcgcacc gtgggacagg
     5641 tggacagccg ttatccgtca ggacctggcg ctgctgctgg aacgtctgcc agggctggaa
     5701 aatctggcat ggaacgacga cacaccgttc gccgacgagg tgacgcgcaa ctggatagcg
     5761 cagcaggtca tgatgcggga agacggcgca tggcttgcag ggaaagccgc tgtaccgaca
     5821 gacgacgcca caaatgacgt actggcgctg gagccggaag cactggagat ggcagacagt
     5881 cagggcgtgg aagcggctct gggctggata cagacccgtc cgggcatcac cacggcacgc
     5941 cagcgcctgt tattgcgact gctgatggcg cgggtggcgg aacagtacgg taaaaacgaa
     6001 atggcgctcc tgctgctgga agaactggat accgccgcac aggggctgac actgacgcag
     6061 tgggagccgg acctgctgtt tgaggtaaaa gcccgacaac tgaaactgct gcgcctgcgg
     6121 gcgcaccgct atgcggataa agccctgctg aaccgaaaaa tggaaatcct gctgggaacg
     6181 ctggtgacaa tcgatccggt acgggcagcg gtattgtgtg atacgcaaca taaggactga
     6241 tggcaatggg aacaggctac tttctggtca ggggggacaa aaccacctgc ggcgggaaga
     6301 ttattggagg cgcggacgac catacgatca tgggtatccc acaggcacgg gatatggacc
     6361 gggtcacctg cggcagatat ccgggaatgt ttattattgt gggcggcgtt cctgaaacag
     6421 atattcacgg caggctgatg gcggggacgc tggacagcca gagcagttgc ccctgtaagg
     6481 cacggtttat tgcgtcgatg atggatgata cgtatgagac ggatgacggg ggaagtgaag
     6541 cggaacaaca tgcgcagtcg gcgaaaaagg atctgacttc tggttctgat tcatcctctg
     6601 atgatgtaaa actggattac agaattaaat tatcaggtaa taagatactg acgccactga
     6661 atatacctga ttataaagaa atgataagcg gtggtagtac gaagaataca gaaaaaatag
     6721 attttactat cacaaataaa ggcgatgagg ctgaaggact ttctctggaa gtgcttgatg
     6781 gtaatgaagt gatttacagt gaacagcaga ccggtaaata ttttgataag ggagagcatg
     6841 cctggcaatg ggatggctac agtaatcaag gcatactgga tacaactaag ctaaaaagca
     6901 aatcactact cgtaagactg attgctttat gtggtgatat gatgataaaa gttgattatc
     6961 cacttcataa ttcaccagag gaacaaaaat gggtcgatgt aaaggtagac agaaaacaaa
     7021 aaacagtaga tataatatgg cgactggcag ttagtgatgg agggataaaa ggaagtaatc
     7081 ctaagttatc cccagttcct tataacgact tggttaacct gaccaaaaat ggagttgaat
     7141 tttattggtc tcgaaatgga agtcgcggcg gggggattgg tgaaaatatt gttacagcga
     7201 ttggtgtttt taaagtaaat gtaaaggctg agataaatat tactccatcc atgcgaactt
     7261 tttctttgat atcatcatta gatcctgact ttcaggcatc cgttagtcta tcaggatttg
     7321 aaaaaatata ttataactac ggtgatagtt ataaggatat tcaagatgaa ttacaggcgc
     7381 ttcttgacgc taataacaga tataaatggg atagtgctca tgaaatgggg cataaagtac
     7441 tcgatgaata tggagagggt tctagccctg attattcgtg gactcataaa ggaacatcta
     7501 ctcttatgca aaaaacaata ccagggaatg ttatgcccgc tcagggagag attgatgtaa
     7561 tgaaatatgg aaaatatcgt cctgacatgt atactcgatt ggttgcagca gatgaagatg
     7621 ttcagggatt gatctggtta agcaggataa aatttgatga ctagagtagt tattgcttta
     7681 ttatttcttg ccggatgtac ttcgcaaatg aagtattcca atgtcgtcag gattaggaat
     7741 gtatgttcct cagatattaa tgtgtttatt cctgagtatg tttttggagg taatgttatt
     7801 aagcaacatc atatttactt acctgttaat gggattgatg ttttgtctta tttttatact
     7861 gaaaacatca atgatggttt ccaatatgaa gaagcattca ccgtaaataa agatagctcg
     7921 ggtaattttg attatccgtt tgttatctta tcatcaggac gtgaaaaaaa atttgggaaa
     7981 aacgatctaa ttgaattatc cagggacatt tcaacaaaag acgataagaa gcatggggtt
     8041 gtaaagtaca tgatagatag tcaggatatt
//
LOCUS       DAZFWP020000197         8059 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFWP020000197 DAZFWP020000000
VERSION     DAZFWP020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.198"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..70
                     /locus_tag="ACHK0Z_RS22300"
                     /old_locus_tag="pgaptmp_004460"
     CDS             <1..70
                     /locus_tag="ACHK0Z_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="DNA-damage-inducible protein J; antitoxin of
                     YafQ-DinJ toxin-antitoxin system; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin,
                     RelB/DinJ family"
                     /protein_id="HGU4504359.1"
                     /translation="SEAGVDVHKAKDADDLFDKLGI"
     gene            73..351
                     /gene="yafQ"
                     /locus_tag="ACHK0Z_RS22305"
                     /old_locus_tag="pgaptmp_004461"
     CDS             73..351
                     /gene="yafQ"
                     /locus_tag="ACHK0Z_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU4504360.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            507..1247
                     /gene="dpaA"
                     /locus_tag="ACHK0Z_RS22310"
                     /old_locus_tag="pgaptmp_004462"
     CDS             507..1247
                     /gene="dpaA"
                     /locus_tag="ACHK0Z_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU4504361.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1218..1985)
                     /gene="yafJ"
                     /locus_tag="ACHK0Z_RS22315"
                     /old_locus_tag="pgaptmp_004463"
     CDS             complement(1218..1985)
                     /gene="yafJ"
                     /locus_tag="ACHK0Z_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU4504362.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2191..2769)
                     /gene="lpcA"
                     /locus_tag="ACHK0Z_RS22320"
                     /old_locus_tag="pgaptmp_004464"
     CDS             complement(2191..2769)
                     /gene="lpcA"
                     /locus_tag="ACHK0Z_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU4504363.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3009..5453
                     /gene="fadE"
                     /locus_tag="ACHK0Z_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             3009..5453
                     /gene="fadE"
                     /locus_tag="ACHK0Z_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU4504364.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5496..5942)
                     /gene="ivy"
                     /locus_tag="ACHK0Z_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             complement(5496..5942)
                     /gene="ivy"
                     /locus_tag="ACHK0Z_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU4504365.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6123..6890
                     /gene="yafV"
                     /locus_tag="ACHK0Z_RS22335"
                     /old_locus_tag="pgaptmp_004467"
     CDS             6123..6890
                     /gene="yafV"
                     /locus_tag="ACHK0Z_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU4504366.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(7114..>8059)
                     /locus_tag="ACHK0Z_RS22340"
                     /old_locus_tag="pgaptmp_004468"
     CDS             complement(7114..>8059)
                     /locus_tag="ACHK0Z_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4504367.1"
                     /translation="ENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAI
                     DGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKG
                     KIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYA
                     ISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCGAVSFRFIIAEQKKEPEMTVR
                     YYISSADLTAEKFATAIRNHWHLENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIA
                     INILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1920 a         2011 c         2084 g         2044 t
ORIGIN      
        1 cagcgaagct ggcgttgatg ttcataaggc caaagacgcc gatgatttat ttgataaatt
       61 aggaatttaa atatgattca aagggatatt gaatactcgg gacaattttc aaaggatgta
      121 aaacttgcac aaaagcgtca taaggatatg aataaattga aatatcttat gacgcttctt
      181 atcaataatg ctttaccgct tccagctgtt tataaagacc acccgctgca aggttcatgg
      241 aaaggttatc gcgatgctca tgtcgaaccg gactggatcc tgatttacaa acttaccgat
      301 aaacttttac gatttgagag aactggaact cacgcggcgc tctttgggta aagcacgctg
      361 ccgatgaaat ttgtaaaccc tgcgccgaca ggcttgcgta aaactctttt ggtgataatg
      421 tgagccttcc aaatccagac tattccgatt tgggatctat tttgccgtcc tggcgtgtgt
      481 aaccgtttta tcaaggaata agcagtatgc gtaaaatcgc attaattctt gcgatgttgt
      541 tgatcccgtg cgtttcgttt gccggtttgc tgggcagcag tagctcgacc acgccagtga
      601 gcaaagagta taagcagcag ttgatgggat cccctgtcta catccagatc ttcaaggaag
      661 aacgtacgct cgatctctac gtcaaaatgg gcgagcaata tcagctgctc gacagttata
      721 aaatctgtaa atattccggc ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc
      781 cggaagggtt ttatagcgtc cagcgtaatc agttaaaacc agacagccgt tactacaaag
      841 cgattaatat tggtttcccc aatgcctatg accgtgcgca tggttatgaa gggaaatacc
      901 tgatgatcca cggcgattgt gtttccatcg gctgctacgc aatgaccaat cagggtattg
      961 atgagatctt ccagttcgtt actggtgcgc tggtgtttgg tcagccgagc gtgcaagtga
     1021 gtatttaccc gttccgcatg accgacgcca atatgaagcg ccataaatat tccaacttta
     1081 aggacttctg ggagcagctg aagccgggct acgactactt tgagcaaacc cgtaagccac
     1141 cgaccgtttc tgtggtcaat ggccgttacg tggtcagcaa gccgttaagc cacgaagttg
     1201 tgcagccaca actggcatca aactacacgc tccccgaggc aaaataagcg ccattcgcct
     1261 ggcataatct tttgccaggt ttcattgccc gtcagcggct gtgttgcaat caccgtgacc
     1321 acatcattcg gtgtggtctg cgagctgaag tcgatttcca catcctgatc cagcagcgtt
     1381 gccacgccaa acggcgcgcg gcgggtgatc cagtgtaaat tagtcgagca atacgccatc
     1441 acatagcgcc cgtcagaaag cagcatgttg aaaacgccct tctgccgcag ttcatccgcc
     1501 agtgaggcga tatatttaaa cactgccgcc atgttgcccg gtgtgcgcgg gtaacgctgc
     1561 gttaatttat gcaggagcca gcagaaggct ttttcgctat cggtttcacc gaccgggcgg
     1621 aagttgccgg tttccagtga tttgtagccc gtcagttgtc cgttatgggc ataagtccag
     1681 ttacgccccc ataactcgcg ggtaaatggg tgagtatttt ccagcgccac ctcgccccga
     1741 ttagcctggc gaatatgagc caccaccgaa caggatttta tcgggtagtc ctggacaagt
     1801 ttggcgatgg gggaattaaa gctgggttgt ggatctttaa atgtgcgaca gcctttacct
     1861 tcgtaaaagg taatgcccca gccatcttta tgtggcccgg ttccaccacc acgctgtaca
     1921 agtccggtga aactaaagca gatatcggta gggacgttgg cgctcatccc gagcaattcg
     1981 cacatgggta acctccacaa cagcgggagc gaatggcccc cggcgaagat tgatttaaga
     2041 attttcaggt cggatgcggc gtaaacgaat tattcggcct acggttcggg tacagatttg
     2101 taggcatgat aagacgcgtc agcatcgcat caggcatcag cgcacaagtg ccggatgcgg
     2161 cgtaaacgcc ttatccagcc tacgccagac ttacttaacc atctcttttt caattaactg
     2221 gatcaggata tggatcactt taatgtgaat ctcctgaatg cggtcggcat aaccaaagtg
     2281 cggtacgcga atttcgatat ccgccgtgcc agccattttg ccgccgtctt taccggtcag
     2341 ggtgatcact ttcatcccct tctcacgcgc cgctgcgatc gctttgatca cgtttgcaga
     2401 gttaccggag gtggagatcc ccagcagtac atcgccttcg cgacctaccg cttcaacgta
     2461 gcgggagaaa atatcattga aaccgaaatc attaccgacg caggaaatat gactaacgtc
     2521 agaaatagca atcgccgggt agcccggacg gttttcacgg tagcgaccgg tcaactcttc
     2581 ggcaaagtgc atagcgtcgc aatgggaacc gccgttgccg caggaaagca ctttgccacc
     2641 ggctttaaag ctgtctgcta acaggaccgc cgcgcgctga atggcgtgaa tattggcgtc
     2701 atcttttaaa aagttagcca gcgtttccgc cgcttcgttc agttcgttac gaataagatc
     2761 ctggtacatg aggatatcct tcagcataaa tgtaatagac aaaatgcagt gtaccggata
     2821 ccgccaaaag cgagaagtac gggcaggtgc tatgaccagg actttttgac ctgaagtgcg
     2881 gataaaaaca gcaacaatgt gagctttgtt gtaattatat tgtaaacaca ttgctaaatg
     2941 tttttacatc cactacaacc atatcatcac aagtggtcag acctcctaca agtaaggggc
     3001 ttttcgttat gatgattttg agtattctcg ctacggttgt cctgctcggc gcgttgttct
     3061 atcaccgcgt gagcttattt atcagcagtc tgattttgct cgcctggaca gccgccctcg
     3121 gcgttgctgg tctgtggtcg gcgtgggtac tggtgcctct ggccattatc ctcgtgccat
     3181 ttaactttgc gcctatgcgt aagtcgatga tttccgcgcc ggtatttcgc ggtttccgta
     3241 aggtgatgcc gccgatgtcg cgcactgaga aagaagcgat tgatgcgggc accacctggt
     3301 gggagggcga cttgttccag ggcaagccgg actggaaaaa gctgcataac tatccgcagc
     3361 cgcgcctgac cgccgaagag caagcgtttc tcgacggccc ggtagaagaa gcctgccgga
     3421 tggcgaatga tttccagatc acccatgagc tggcggatct gccgccggag ttgtgggcgt
     3481 accttaaaga gcatcgtttc ttcgcgatga tcatcaaaaa agagtacggc gggctggagt
     3541 tctcggctta tgcccagtct cgcgtgctgc aaaaactctc cggcgtgagc gggatcctgg
     3601 cgattaccgt cggcgtgcca aactcattag gcccgggcga actgttgcaa cattacggca
     3661 ctgacgagca gaaagatcac tatctgccgc gtctggcgcg tggtcaggag atcccctgct
     3721 ttgcactgac cagcccggaa gcgggttccg atgcgggcgc gattccggac accgggattg
     3781 tctgcatggg cgaatggcag ggccagcagg tgctggggat gcgtctgacc tggaacaaac
     3841 gctacattac gctggcaccg attgcgactg tgcttgggct ggcgtttaaa ctctccgacc
     3901 cggaaaagct gctgggtggc gcagaagatt taggtattac ctgtgcgctg atcccgacca
     3961 ccacgccggg cgtggaaatt ggtcgtcgcc acttcccgct gaacgtaccg ttccagaacg
     4021 gaccgacgcg cggtaaagat gtcttcgtac cgatcgatta catcatcggt gggccgaaaa
     4081 tggccgggca aggctggcgg atgctggtgg agtgcctctc ggtaggccgc ggcatcaccc
     4141 tgccttccaa ctcaaccggc ggcgtgaaat cggtagcgct ggcaacgggc gcgtatgctc
     4201 acattcgccg tcagttcaaa atctctatcg gtaagatgga agggattgaa gagccgctgg
     4261 cgcgtattgc cggtaatgcc tacgtgatgg atgctgcggc atcgctgatt acctacggca
     4321 ttatgctcgg cgaaaaacct gccgtgctgt cggctatcgt taagtatcac tgtacccacc
     4381 gcgggcagca gtcgattatt gatgcgatgg atatcactgg tggcaaaggc attatgctcg
     4441 ggcagagcaa cttcctggcg cgtgcttacc agggcgcacc gattgccatc accgttgaag
     4501 gggccaacat tctgacccgc agcatgatga tcttcggaca aggagcgatt cgttgccatc
     4561 cgtacgtgct ggaagagatg gaagcagcga agaacaatga cgtcaacgcg ttcgataaac
     4621 tgttgttcaa acatatcggt cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc
     4681 tgacgcgcgg tttaaccagc agcacgccaa ccggcgatgc cactaaacgc tactatcagc
     4741 acctgaaccg cctgagcgcc aacctcgccc tgctttctga tgtctcgatg gctgtgctgg
     4801 gcggcagcct gaaacgtcgt gagcgcatct cggcacgtct gggggatatt ttaagccagc
     4861 tttacctcgc ctctgccgtt ctgaagcgtt atgacgacga aggccgtaat gaagccgacc
     4921 tgccgctggt gcactggggc gtacaagatg cgctgtatca ggctgaacag gcgatggatg
     4981 atttactgca aaacttcccg aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc
     5041 cgaccggacg tcattatctg gcaccttctg acaagctgga tcataaagtg gcgaagattt
     5101 tacaagtgcc gaacgccacc cgttcccgca ttggtcgcgg tcagtacctg acgccgagcg
     5161 agcataatcc ggttggcttg ctggaagagg cgctggtgga tgtgattgcc gccgacccaa
     5221 ttcatcagcg gatctgtaaa gagctgggta aaaacctgcc gtttacccgt ctggatgaac
     5281 tggcgcacaa cgcgctggcg aaggggctga ttgataaaga tgaagccgct attctggtga
     5341 aagctgaaga aagccgtctg cgcagtatta acgttgatga ctttgatccg gaagagctgg
     5401 cgacgaagcc ggtaaagttg ccggagaaag tgcggaaagt tgaagccgcg taattgaata
     5461 acggagccga aaggctccgt ttctttatcc gctaattatt taaaattaaa gccatccgga
     5521 tggttttcca ggctgccggt caacgccgcg aacagcaccg ttttaccatc aatcgaaagc
     5581 gcatcgttca cattcagcca ggtgagtttc tcttgcgacg ttttctcatc aatagtcgag
     5641 aacagccccg tcatctgatt agatttctcg gaccacatca cagcgatacg ttgcgagcca
     5701 cagtcatgcg gtttgcacgc gctcatcacc tgatacgtct catctcccaa cgttacggtt
     5761 tgtgcgggag tataagtacc gcctttcatc acccaggcag gcagcttatg cccttgtacc
     5821 atctgattaa atgcagcttt ggtggtttcg ccctttgcaa ggctgctaat ggttaaatca
     5881 tcctgcgcca ttgcactggt ggcgatgacc agagcggcga ctgtcgttat tgccttaaac
     5941 atcattcctc ccgagcttat cctgcccatg ttattaacct tctaaagcct ggcgataaca
     6001 cgataatttg caaatcagat atttccaata tcagcatttg ttatcaattt attctgcgct
     6061 aaggcaactg cctgataagg tgttaaagtt ataaaaccct tcgcataaag gagttgtaca
     6121 ccgtgcctgg tttgaagatt acgcttttgc agcaaccact ggtgtggatg gatggccctg
     6181 ccaacctgcg tcattttgat cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc
     6241 taccggagat gtttaccagc ggctttgcca tggaagcggc agcttcgtcg ctagcacaag
     6301 atgacgtagt gaactggatg acagctaagg cgcagcagtg caatgcgctg attgcaggca
     6361 gtgttgcatt acaaacggag tctggttcgg ttaaccgctt tttgctggtt gagccgggcg
     6421 gcacggtaca tttttatgat aagcgtcatc tgttccgcat ggcagatgag catctacatt
     6481 ataaagcggg caatgcgcga gtgattgtgg aatggcgcgg ctggcgtatt ttgccgctgg
     6541 tgtgctacga cttacgtttt cctgtgtggt cgcgcaatct caacgattat gacctcgccc
     6601 tgtacgtcgc caactggcct gctccgcgct ctctgcactg gcaggcattg ctgacggccc
     6661 gcgcgattga gaatcaggcg tatgtggcgg gatgcaatcg cgtcggcagc gatggcaacg
     6721 gctgccatta tcgcggtgac agccgggtga ttaatccaca aggcgagatt atcgctactg
     6781 ccgacgcgca tcaggcaacg cgcattgatg ccgagctgtc gatggcggtg ttaagggaat
     6841 atcgcgataa gtttccggca tggcgggatg cggatgagtt taggttgtag tgagtggggg
     6901 ctgtatttag ggcggggaat tcagaaaggg atgtttagtg tgctgagcga gagttaaata
     6961 ataaaaaact gggaagattt aataatcccc ccgcagggca agagcatgaa ttaaatttgt
     7021 gttaaatttt tcattttgtc agttcgaaac ccaagctctg agcaggatgc aagcacttct
     7081 tctatataac atgtgtttaa cgccagggca agattacgaa agcccgctcc ccgcaaggac
     7141 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     7201 gaatacctta tcattcgtca aaatattaat agcaatgtgc cgtatccctg aaaataattc
     7261 tgctgcattt cctcttctta ttttgcagtc gtcttcattc attaccacgt ccagacgcca
     7321 gtgcagctta ttctccaagt gccagtgatt tcggatcgct gtggcgaatt tctcagcggt
     7381 taaatcagca gaactgatat aatatctgac cgtcatttct ggctctttct tttgttctgc
     7441 tattatgaac cgaaaggaga ctgccccgca taatttcttc agccctttcc attcaaacgt
     7501 gaaatcaata agttcatcag ggacatcgca aacaatatga agacggattt cttctctgcc
     7561 gtgactcttt tcactaattg cgtaactgtc atgctctgga ttatttaatt ctttcagcgg
     7621 aaatttttcc tcaaaggctt tatttagccg cccctggttt ccttttaccg cgaataaata
     7681 atcacctccc tgtttttgta tcttctctgc aatatctttc tggcaaccca tcgcatcagt
     7741 tgtgatgatt tttcctttaa tatccagcat gttaagaagt tctgggatag ctgtaatctc
     7801 attagatttc tcatccgtcc tgatctgtcc gatgaccaga ctgtgcattg ttgagaacgc
     7861 actaatgaca tgaatcgctc ccctgcggcg actcttgtca taagagtgcc ggagcgtttt
     7921 tccatcaatt gcgatgacgt ctttatcatc tgaagaatgg cagtcacgca tccagttaat
     7981 aaagcactca tgaaattttg caggactgat acaggataca actctggcaa tagtatcgtg
     8041 aacaggaata ccattttca
//
LOCUS       DAZFWP020000198         8022 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFWP020000198 DAZFWP020000000
VERSION     DAZFWP020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.199"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            336..1364
                     /gene="torT"
                     /locus_tag="ACHK0Z_RS22345"
                     /old_locus_tag="pgaptmp_004469"
     CDS             336..1364
                     /gene="torT"
                     /locus_tag="ACHK0Z_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU4504368.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1337..2029)
                     /gene="torR"
                     /locus_tag="ACHK0Z_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             complement(1337..2029)
                     /gene="torR"
                     /locus_tag="ACHK0Z_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU4504369.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2159..3331
                     /gene="torC"
                     /locus_tag="ACHK0Z_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             2159..3331
                     /gene="torC"
                     /locus_tag="ACHK0Z_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU4504370.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3331..5877
                     /gene="torA"
                     /locus_tag="ACHK0Z_RS22360"
                     /old_locus_tag="pgaptmp_004472"
     CDS             3331..5877
                     /gene="torA"
                     /locus_tag="ACHK0Z_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU4504371.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGVIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5874..6473
                     /gene="torD"
                     /locus_tag="ACHK0Z_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             5874..6473
                     /gene="torD"
                     /locus_tag="ACHK0Z_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU4504372.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(6566..6871)
                     /gene="cbpM"
                     /locus_tag="ACHK0Z_RS22370"
                     /old_locus_tag="pgaptmp_004474"
     CDS             complement(6566..6871)
                     /gene="cbpM"
                     /locus_tag="ACHK0Z_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU4504373.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(6871..7791)
                     /gene="cbpA"
                     /locus_tag="ACHK0Z_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             complement(6871..7791)
                     /gene="cbpA"
                     /locus_tag="ACHK0Z_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU4504374.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         1938 a         2010 c         2186 g         1888 t
ORIGIN      
        1 aggcatagcc ataatgcatg atggtgtggc gtggcaaagc agagatatac aaagtctgcc
       61 acatgttttt atgacaaaaa attagcgcaa gaagacaaaa aatcaccttg cgctaatgct
      121 ctgttgtagg tcactaacca catctaactc attgattaat agtgactcta gatgtagtgt
      181 tttatcgtat tttgcagtct gttatttaat catgaattca tgtaacaata tgatttttaa
      241 aatatttgtg tgtatttgtc atcctttagg tgaaaaaggt tgagtcgcaa agcggaatgc
      301 atctagcata aagccttatt attgatgagg ctatcatgcg cgtactgcta tttttacttc
      361 tttccctttt catgttatcg gcattttcgg ctgataacct gttgcgctgg catgatgcgc
      421 agcattacac ggtgcaagcc tctacgccgc ttaaagccaa acgcgcatgg aaactgtgcg
      481 cgctttatcc cagcctgaaa gattcatact ggttatcgtt gaactatggt atgcaggagg
      541 cagctcgccg ctatggtgtg gatttaaaag tgctggaggc aggcggctac agccagttgg
      601 ctacccagca agcacaaatc gaccagtgta aacagtgggg cgcagaggcc attttgctcg
      661 gtagtagcac tacgtcattt cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg
      721 aactggtaaa tgctcttgat gatcccctgg tgaaaagccg cgttggtgta ccctggtttc
      781 agatgggcta tcaacctggg cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg
      841 tgctgttgat gcccggaccc gataatgccg ggggcagtaa agagatggtc gagggttttc
      901 gcgcagccat tgccggaagc ccggtgcgta ttgttgatat tgcgcttggt gataacgata
      961 ttgaaatcca gcgtaacctg ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg
     1021 ttgccggaac ggccattgcg gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc
     1081 cgcttaccgt ggtgtcgttt tatctttcac atcaggtgta tcgcgggctg aagcggggaa
     1141 gagtgattat ggctgccagc gatcaaatgg tctggcaggg ggaactggcg gttgagcagg
     1201 ccatcaggca attacagggg caatcggtgt ctgataatgt cagcccaccg attttagttc
     1261 tgacgccgaa aaatgccgac cgtgaacata ttcgccgctc gctgtcacca gggggatttc
     1321 gtccggtcta tttttatcag cacacatcag cggctaagaa ataaccttca ccatgttgcg
     1381 tcaccagtaa atccgcgctg agtttatgac gtaaacgacg aattaacaca tcgacggtgc
     1441 gcaggtcagg gttttccacc cgacgcgcag aaagcatacg tagcagacgt tcacggctga
     1501 gaatttcgcc cggattcgtc acaaatgcca ccaacatttc atactctgcg cgggtcagtt
     1561 taatcggctc gccatcccgc tccagcgtat ggcgcgacac attcaggcaa taaccggcaa
     1621 agcgatagca gttgtcctga gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga
     1681 gcagattttt cacccgtact accagttcgc gcagttccag cggtttggtg acgtaatcgt
     1741 ctgcgcccat ttccagccca acaatacggt caatccgatc gctgcgtccg gtaaccagaa
     1801 taatccccac cgttgagcgt tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg
     1861 gtaagttgat atccagcaga attaaatcta ccggctgatt ctgcataatt tcccgcagcc
     1921 cggcaccgct cgctgtaacg gaaacggtat acccctcctg agtgaagtaa gattgtaatc
     1981 gcgcctgggt aaccggctca tcttcaacaa taacaatgtg atgtggcatc agagggtttt
     2041 actcattctg ttcatatctg ttcatattct gccgtaagcc gttcatcctg accagtgccg
     2101 ctgttcatat ttgctcatta agatcgcttc actaaaccat aattttacag gggctattat
     2161 gcggaaactc tggaacgcgc tacgccgacc cagtgctcgt tggtcggtac tggcgctggt
     2221 cgcaattggg attgtgattg gcattgcgct gattgtattg ccacacgttg ggatcaaagt
     2281 caccagcaca accgaatttt gtgtcagttg ccacagtatg caaccggtgt atgaagaata
     2341 taaacagtcg gtgcatttcc agaacgcctc cggcgtgcga gctgaatgcc atgactgtca
     2401 tatcccgccg gatattccag gcatggtgaa gcgcaaactg gaagcgagca atgatatcta
     2461 ccagaccttt attgctcact ccattgatac acctgaaaaa ttcgaagcca aacgcgcgga
     2521 acttgccgag cgtgaatggg cgcgaatgaa agaaaacaac tcggcaacct gccgctcctg
     2581 ccataactac gatgcgatgg atcatgcgaa gcagcatcct gaagcggcac gtcagatgaa
     2641 ggtggcagcg aaagataatc aatcctgcat cgactgtcat aaaggtattg cccaccagtt
     2701 accggatatg agtagcggct tccgtaagca gttcgatgag ctgcgcgcca gtgctaatga
     2761 cagtggtgac acgctgtact ctattgatat taagccaatt tatgcggcga aaggcgataa
     2821 agaagcctct ggttctctgc tgcctgcttc ggaagtgaaa gtccttaaac gtgacggcga
     2881 ctggctgcaa attgaaatta ccggctggac ggaaagcgcc ggacgtcagc gtgtactcac
     2941 ccaattccca ggtaaacgca tctttgttgc ctcgattcgt ggtgatgtgc agcagcaggt
     3001 aaaaacgctg gagaaaacca ccgttgccga caccaatacc gagtggagca agttgcaggc
     3061 cactgcgtgg atgaagaaag gcgacatggt gaacgatatc aaaccgatct gggcttatgc
     3121 ggattcgttg tacaacggca cctgtaacca gtgccacggc gcaccggaaa tcgcccactt
     3181 tgacgctaac ggttggatcg gcacgctcaa cggcatgatt ggctttacca gtctcgataa
     3241 acgtgaagaa cgcaccttgt tgaaatatct gcaaatgaat gcgtctgaca ccgcaggtaa
     3301 ggctcacggc gataagaagg aagaaaaata atgaacaata acgatctctt tcaggcatca
     3361 cgtcggcgtt ttctggcaca actcggcggc ttaaccgtcg ccgggatgct ggggccgtca
     3421 ttgttaacgc cgcgccgtgc gactgcggcg caagcggcga ctgacgctgt catctcgaaa
     3481 gagggcattc ttaccgggtc gcactggggg gctatccgcg cgacggtgaa ggatggtcgc
     3541 tttgtggcgg caaaaccgtt cgaactggat aaatatccgt cgaaaatgat tgccggattg
     3601 ccggatcacg tacacaacgc ggcgcgtatt cgttatccga tggtacgcgt ggactggctg
     3661 cgtaagcgcc atctcagcga tacctcccag cgcggtgata accgttttgt gcgcgtgagc
     3721 tgggatgaag ccctcgacat gttctatgaa gaactggaac gcgtgcagaa aactcacggg
     3781 ccgagtgcct tgctgaccgc cagtggttgg caatcgacgg ggatgttcca taacgcttcg
     3841 gggatgctgg cgaaagctat tgccttgcat ggtaatagcg ttggtacggg cggagattac
     3901 tctaccggtg ctgcgcaggt gatcctgccg cgcgtggttg gctcaatgga agtgtatgaa
     3961 cagcaaacct cctggccgct ggtattgcag aacagcaaaa ccattgtgct gtggggctct
     4021 gatttgctga aaaaccagca agcgaactgg tggtgcccgg atcacgatgt ttatgaatat
     4081 tacgcgcagc taaaagcgaa agtcgccgcc ggtgaaattg aggtcatcag catcgatccg
     4141 gttgtcacat ccacccatga gtatctgggg cgcgagcatg tgaagcacat tgcggttaac
     4201 ccgcaaactg atgtgccgct gcaactggcg ctggcgtata cgctgtacag tgaaaacctg
     4261 tacgacaaaa acttcctcgc taactactgt gtgggttttg agcagttcct gccgtacctg
     4321 ctgggtgaga aagacggtca gccgaaagat gccgcatggg ctgaaaaact gaccggcatt
     4381 gatgccgaaa ccattcgtgg gctggcgcgg cagatggcgg cgaacagaac gcaaattatt
     4441 gctggctggt gcgtgcagcg tatgcagcac ggtgaacagt gggcgtggat gattgtggtt
     4501 ctggcggcga tgctggggca aattggcctg ccaggtggtg gctttggttt tggctggcac
     4561 tataacggcg caggcacgcc ggggcgtaaa ggcgttattc tgagtggttt ctccggctct
     4621 acgtcgattc cgcctgttca cgacaacagt gattacaaag gctacagcag caccattccg
     4681 attgcccgtt ttatcgatgc gatcctcgaa ccggggaaag tgatcaactg gaacggtaaa
     4741 tcggtaaaac tgccgccgct gaaaatgtgt atttttgccg gaactaaccc attccatcgc
     4801 catcagcaga tcaaccgcat tattgaaggc tggcgcaagc tggaaacggt tatcgccata
     4861 gataaccagt ggacctcaac ctgccgcttt gccgatatcg tgctgcctgc gaccacgcag
     4921 tttgagcgta acgatctcga ccagtacggc aaccactcca accgtggcgt tatcgccatg
     4981 aaacaggtgg tgccgccgca gttcgaggcg cgcaacgact ttgatatttt ccgcgagctg
     5041 tgccgtcgct ttaatcgcga agaagccttt accgaagggc tggacgaaat gggctggctg
     5101 aaacgcatct ggcaggaagg tgtacaacaa ggcaaaggac gcggcgttca tctgccagcg
     5161 tttgatgact tctggaataa caaagagtac gtcgagtttg accatccgca gatgtttgtt
     5221 cgccaccagg cattccgcga agatccggat ctcgaaccgc tgggcacgcc gagtggcctg
     5281 attgagatct actcgaaaac catcgccgat atgaactacg acgattgtca ggggcatccg
     5341 atgtggtttg agaaaatcga acgctcccac ggtgggcccg gctcgcagaa gtatccgttg
     5401 catctgcaat ctgtgcatcc ggatttccga cttcactcgc agttatgtga gtcggaaact
     5461 ctgcgtcagc aatatacggt agcgggtaaa gagccagtgt tcattaaccc gcaggatgcc
     5521 agcgcgcgcg gtattcgtaa cggtgatgtg gtacgcgtct ttaacgctcg cggtcaggtg
     5581 ttggcagggg cagtagtttc tgaccgctat gcacccggcg tggcacgaat tcacgaaggg
     5641 gcatggtacg atccagataa aggcggcgag cctggtgcgc tgtgcaaata cggtaacccc
     5701 aacgtgctga ccatcgacat cggtacttcg cagctggcgc aggcgaccag tgcgcacact
     5761 acgctggtgg aaattgagaa gtacaatgga gcagtggagc aggtgacggc gtttaacggc
     5821 cctgtggaga tggtggcgca gtgcgaatat gttcccgcgt cgcaggtgaa atcatgacca
     5881 cgctgacagc acaacagatt gcctgtgttt acgcctggct ggcgcagttg ttctcccgtg
     5941 aactggacga tgaacaactg acgcaaatcg ccagtgcgca gatggctgaa tggttttcgt
     6001 tgctgaaaag cgaaccgccg ctggctgcgg cggtgaatga gctggaaaac tgtattgcca
     6061 cgctgacagt acgtgacgat gcccgtctgg aactggccgc tgacttttgc ggcctgtttc
     6121 tgatgaccga caaacaagcg gcgctgccgt atgcatcggc ctacaaacag gacgagcaag
     6181 agattaaacg cttgttagtt gaggcaggga tggaaaccag cggcaatttc aacgaaccgg
     6241 cagaccatct ggcgatctat ctcgagttgt tgagtcatct gcatttttcg ctgggagagg
     6301 ggaccgttcc tgcgcgaaga atcgacagtt tgcggcaaaa aacactgacg gcgctgtggc
     6361 aatggttacc agagtttgct gcgcgttgcc atcagtatga cagctttggt ttttacgcgg
     6421 cactaagcca gttattgctg gtgttagtgg agtgcgacca ccaaaacaga taacgtcgtt
     6481 tatgcgcctg aaaagacgcg tttagcgtcg catcaggcat tatggcgcag ttgccggatg
     6541 cgtcatcaca tccggcaaac acacatcacg gatgagctac aaaccgggaa agccgctggc
     6601 gtagcaggcg gttttcctgc ttcaggtgtg caatatcatc cattaacgtc agcgccaccg
     6661 cgatccccgg ccagtccaga gccagttcat gacgcaggcg taccgcgcgt tgcaccacaa
     6721 tggcggcatg gtcgtcaaat acccaggttg tttcctggat ctcacgcggt tcaaccaccc
     6781 ccaaaccgac aatttcattc aactcctctt cagagatgcc ggtatgcagg caaaattcgg
     6841 taatagtaaa agtcaccgta acattagcca tcatgctttc ccccaatctt tacgtggatc
     6901 aaaagacgac tgggcgtctg ccagttgctg ccacagcgcg gcagtgtttt catccggttt
     6961 cggcggcatc acgattttca gtaccgcata cagatcgccg gtctgttttt tgctcaccag
     7021 acctttgcct ttaacgcgca atcgttgccc ggcctgactg cctggcggga tagtcagcaa
     7081 aatgctttct ttcagtgttg gaacggtgac tttagcaccc agcgccgctt cccacgggct
     7141 aaccggcacc acaatttcca gatcctggcc gacaatatcg aacagcggat gtggcgcaat
     7201 atgaatcacc agccacaaat cgccatttgg accaccgttt tcgcccggcg tcccctggcc
     7261 tttcagacgg atgcgttgac cattgccgac gcccgccggg atcttcacat tcagcgtttt
     7321 cgggatttcc tgttcgatca tgccaaaggc gttataaacc ggcaggttat agctgatggt
     7381 acgcttatgc tcagtcagcg tttcttcgag gaataccgcc acttcgattt caatatcgtg
     7441 gccgcgtgcg gcggggcgtt gacggctctg gcgggcatgc tgaccgaaaa ttgacgagaa
     7501 gatatcgtca aaatcttcgg cattaaaact ctgaccgtcg ccatgatgga actgacggtt
     7561 aaattgcgga tcgttgcgat gttgccacat ctgatcatac tcagcgcgac gttgttcatc
     7621 acttaacact tcccaggctt cagcgacctc tttgaagcgg gcttcggcat ccggttcttt
     7681 gctgacatca ggatggtatt tgcgggcaag tcgacgatag gcggtcttga ttgtcttgag
     7741 atcgtccgtc ggtttcacgc ccatgatggc gtaataatcc tttaattcca tagcgttatc
     7801 tcgcgtaaat caacacaaat tgaaggaacc cctgtaaggt aactcctata agtgtagggt
     7861 aatcctcaaa atttcatatg ccaacacaga atatgttatt gaaatcatcg cggagaggag
     7921 gtcgccatca agatgggtta ctgaacatat tttaaacagg tgaaaaaggg tgagcgattt
     7981 ttgatagttg aaccaggcac tttaagttta actggggcgt ca
//
LOCUS       DAZFWP020000199         8012 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFWP020000199 DAZFWP020000000
VERSION     DAZFWP020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.200"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..2128
                     /gene="iha"
                     /locus_tag="ACHK0Z_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             38..2128
                     /gene="iha"
                     /locus_tag="ACHK0Z_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4504375.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2346..2496)
                     /locus_tag="ACHK0Z_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /pseudo
     CDS             complement(2346..2496)
                     /locus_tag="ACHK0Z_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2708..3028)
                     /locus_tag="ACHK0Z_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /pseudo
     CDS             complement(2708..3028)
                     /locus_tag="ACHK0Z_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(3451..4026)
                     /locus_tag="ACHK0Z_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             complement(3451..4026)
                     /locus_tag="ACHK0Z_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4504376.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(4095..4673)
                     /gene="terD"
                     /locus_tag="ACHK0Z_RS22400"
                     /old_locus_tag="pgaptmp_004480"
     CDS             complement(4095..4673)
                     /gene="terD"
                     /locus_tag="ACHK0Z_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU4504377.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4722..5762)
                     /gene="terC"
                     /locus_tag="ACHK0Z_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     CDS             complement(4722..5762)
                     /gene="terC"
                     /locus_tag="ACHK0Z_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU4504378.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(5785..6240)
                     /locus_tag="ACHK0Z_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     CDS             complement(5785..6240)
                     /locus_tag="ACHK0Z_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4504379.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(6263..7420)
                     /locus_tag="ACHK0Z_RS22415"
                     /old_locus_tag="pgaptmp_004483"
     CDS             complement(6263..7420)
                     /locus_tag="ACHK0Z_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4504380.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(7420..8001)
                     /locus_tag="ACHK0Z_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             complement(7420..8001)
                     /locus_tag="ACHK0Z_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4504381.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         2021 a         2047 c         2021 g         1923 t
ORIGIN      
        1 attgtcttgc cggttaacat gatcggagat tagtaatatg cgaataacca ctctggcttc
       61 cgtagtcatt ccctgtctcg gattttcagc cagcagcata gctgctgcag aggatgtgat
      121 gattgtctcg gcatccggct atgagaaaaa gctgactaac gcagccgcca gtgtttctgt
      181 gattagccag gaggaattgc agtccagcca gtaccacgat ctggcggagg ctctgagatc
      241 agtagagggt gtggatgttg aaagtggtac gggtaaaacc ggagggctgg aaatcagcat
      301 ccgaggaatg ccagccagtt acacgctgat actgattgat ggtgttcgtc agggcggaag
      361 cagtgacgtg actcccaacg gtttttctgc catgaatacc gggttcatgc cccctctggc
      421 cgccattgag cgtattgagg ttatcagggg gccgatgtcc acactgtatg gctctgatgc
      481 gatgggcggt gtggtgaata tcattaccag aaagaatgca gacaaatggc tctcttccgt
      541 caatgcaggg ctgaatctgc aggaaagcaa caaatggggt aacagcagcc agtttaattt
      601 ctggagcagt ggtccccttg tggatgattc tgtcagcctg caggtacgcg gtagcacaca
      661 acagcgtcag ggttcatcgg tcacatcact gagcgataca gcagccacgc gtattcctta
      721 tcccacggag tcacagaatt ataatcttgg tgcacgtctt gactggaagg cgtcggagca
      781 ggatgtgctc tggtttgata tggataccac ccggcagcgt tatgataacc gggatgggca
      841 actggggagt ctgacggggg gatatgaccg gaccctgcgc tatgagcgaa acaaaatttc
      901 agctggctat gatcatactt tcaccttcgg aacatggaaa tcgtatctga actggaacga
      961 gacagaaaat aaaggtcgtg agcttgtacg cagtgtactg aagcgcgaca aatgggggct
     1021 tgccggtcag ccgcgggagc ttaaggaatc gaaccttatc ctgaattcat tactgcttac
     1081 ccctctggga gaatctcatc tggttacggt ggggggcgag tttcagagct cgtccatgaa
     1141 agacggagtt gtccttgcca gcacaggtga aactttccgg cagaaaagct ggtcggtatt
     1201 tgctgaggat gagtggcatc tcacggatgc acttgcgctg actgcgggca gccgctatga
     1261 acatcatgag caattcgggg gacacttcag tccgcgtgca tatctggtct gggatgtggc
     1321 agatgcctgg acgctgaaag gcggtgtgac cacgggatat aaggcaccca gaatggggca
     1381 gctacataaa gggattagtg gtgtgtccgg gcagggaaaa acaaatctac ttggtaaccc
     1441 cgacctgaag ccggaagaga gcgtcagtta tgaggctggg gtgtattacg ataaccccgc
     1501 cggtctgaat gccaatgtca caggttttat gactgacttc tccaacaaga ttgtctctta
     1561 ttccataaat gataacacca atagctatgt aaacagcgga aaggcccggt tgcacggtgt
     1621 ggaatttgcc ggcacattgc cgctgtggtc agaggatgtc acgctgtcac tgaattacac
     1681 ctggacccga agtgaacaac gtgatggtga taacaaaggt gcgccgctga gttatacccc
     1741 tgaacacatg gtgaatgcga aactgaactg gcagatcacc gaagaggtgg catcatggct
     1801 gggtgcccgt tatcgcggga aaacaccacg tttcacccag aattattcgt cactgagcgc
     1861 tgtacagaag aaagtgtatg atgagaaagg agaatacctg aaagcctgga cggtggtgga
     1921 tgcaggtctg tcgtggaaga tgacggatgc cctgacgctg aatgctgcgg tgaataacct
     1981 gctcaacaag gattacagtg acgtgagcct gtacagtgcc ggtaagagta cgctgtatgc
     2041 cggtgattac ttccagacgg gatcatcaac aacaggatat gtgatacctg agcgaaatta
     2101 ctggatgtcg ctgaactatc agttctgata ataacaaaac gctatcactg acggtagaat
     2161 acgttgccac tgcaactcct ggcggaacag tggcaacgta ttaggttaag tgcatttccg
     2221 atccgctaat gagatttcgt taccaacaac taatatcgtc acaggaaatg cacggattat
     2281 ttttaactta tcatttacat acttgtccag agtgtaagcg caccgtgaag gacgtggggt
     2341 aaaaattagt ttacagagag agtgacgttc cagggcaaca actctttcat gcggttggca
     2401 ggccaggtgt tgattacact gatcacgtgg cgtagccacg tttccggctc gattccgtta
     2461 agttttgcag ctaccgatca ggctgtacat cactgccgca ctatcgctcg tcatctcaaa
     2521 gtccggtctc gtcagcagga aggtatcatt ctctcccgcc atttttccag gggcccggtc
     2581 agataagtcc ctttgtctat cgctgactcc tgactcataa cccggttagc agaatgcagg
     2641 gtcaccacct cgccacgacc aaatccaaat aagtcaattg caccttctca atcgccattt
     2701 tgtcagtaag cgtacagcct gaactgatgg tatcttcacc atcaatgaca acggtgatcg
     2761 caattttact gacgttcgcc ggaacacgat ccagtgctat ctcaatgctg gcctgctgcg
     2821 aaccggtaac gagcctgact gccccctcag gagaagacaa attattataa aagataaagt
     2881 cagaatcgcc actgaccttt ccctgagcat taagcatgaa caggcaggta tcgggttcgc
     2941 ctttaaagcc ggatttggct gggtactgaa gattcagcct gatcgtagat tgctgaaggg
     3001 gtatgttttg tccggattgt aaattcatat taactctcct gatttttgat tattattaat
     3061 gcgcagcgtt tatatatgtt ccctaggctt agttctggac gctggatatt cggtgaggcg
     3121 taaatatggt atgacaccat ttttcataac gctgaagttt ctatacctgt tgaatttgaa
     3181 ttttcattga ccgggtatct tattttccag ggcccgttcc ttcataagtc gcaaaagtaa
     3241 catatatccg aagggcatgc tgttgatatc agacacggaa tactggcttt aaccagcaac
     3301 cacagataaa cccggggcct gcataaaagg ttcatgccag aattaacata gctctgcttt
     3361 ctgccccccc cctttcccat attcacccgt tgatagcgga tcgataccca aaaaaaaccc
     3421 cggcattgcc ggggtcagac tcagcgtctt ttagacattg acgccgtgct gggaagcaag
     3481 cgctgacaga ccaccggcaa aaccctggcc aacagcttta aacttccact cagtaccatg
     3541 gcgatacagt tcaccgaaga ccattgcggt ttcggttgag gcgtcttcag acagatcgaa
     3601 acgggcaatt tccgtcccgt tgtcgttgtt gtaaacgcgc atgaagctgt tgctcaccat
     3661 gccgaagttt tgtttacgcg cttctgcatc atagatggta acggcaaata ccagtttttt
     3721 gatgtctgct gagactttgg tcagatcgat tttgacctgc tcatcgtcgc cgtcgccttc
     3781 accggtacgg ttgtcgccct ggtgctctac tgcgccatca gggctggttt tattattgaa
     3841 gaaaatgaaa tgggcatctg acagtacttt accgtcttca cctactgcga atacggaagc
     3901 gtccagatca aaaccctgac catcggttac acgggcatcc cagcccaggc caaccatagc
     3961 gacattcatg gttggtgctt ctttggtcag agatacgttg ccgcctttta cgagagaaac
     4021 tgccattttt agctcctgca aacagttgaa tgaggctgaa ttaacagccc cagatatgaa
     4081 aagttacttt tcgatcagga cgcgttaatg ccgtactgag cacatacaga tgccagacca
     4141 ccagcataac cctggcctac tgcgcggaat ttccactcac cattgtggcg atacagctcg
     4201 ccgaacagca tggcagtctc agtggacgca tcttcggtca gatcgtagcg agcgacttca
     4261 gtctggttat cgtcattaac cagacgaata aacgcaccgg atacctgacc aaagctctgg
     4321 cgacgagcct gagcatcgtg gatggtcaca acgaagatga tcttgtcaac ttcagacggg
     4381 acggcgtcca gtttaatttt cagcgattca tcatcaccat cgccctcacc ggtgcggtta
     4441 tcgccggtgt gcgttacgga accgtcggat gacgtcaggt tgttatagaa gatgaaatct
     4501 gaatcgccgc gcactttgcc gtttgaggcc agcaggaatg ctgaagcatc caggtcaaag
     4561 tcctgaccgt ctgttgaacg cgcatcccag ccaaggccca ccaggacatt tttcattgac
     4621 ggagctgctt tactcaggga gacgttcccg cctttggaaa gagaaacact cataaaataa
     4681 cctcttcgat tagtaattgt tcaggttaac acttaagggg attaactccc cttttcctca
     4741 gattcaggtt tgcccgggaa catgacgctt gcgagaatgc ccagcgccag tacacccagt
     4801 acaacataca ggctggttgt tgccgcgatg ctgtaaccat gatgccagat gtgatcggtc
     4861 gcattcaggc cgagttttgc cgcgatgaag aacagcagca cgataacggc cttctccaga
     4921 tgaaccaggt actgtttcag tgcctcaagg acaaaataca gagtacgcag gcccaggata
     4981 gcaaacatca tggcactata gacgataagc ggttcacgac tgacggcaat gattgccggt
     5041 accgagtcaa acgcgaacat tacgtccgag agttcaacca cagccacaca caggaacagc
     5101 ggggtcgcat acagcgccgc ttttttacca cggccaatgg tgacatcgct gttttctggt
     5161 ttcgccagtt cagcatccac ttccttctgg ttaagtagga aggcatgccc tctgagcttc
     5221 ggccagatag ggaagaagcg tttaaccata cggtaagcca gatgctggga gtaatcctca
     5281 atttcatcat cgtcatcacc gcttttaagc atcatgaccg ccgtccaggc aacgataata
     5341 gcgaagacaa cttcaacgta cggccccaga ctcagcaggc tcgtaccgat ggcgacaaag
     5401 atgcccctga agacgatggc accaatgatc ccccagtaga gaacacggtg gcgataacga
     5461 tccggaacgg cgaaccagga gaagattgcc atcatgacga acaggttatc gaccgacagc
     5521 actttctcca gcgcataacc cgtgacaaac agactggcaa cctcagcacc gtggtggata
     5581 tagaggaaac cggcaaatgc catcgcaacc acaacccaga atacggacca gagcgcagca
     5641 ctcttcagcg agattggctt gtcatcacgg tgcataaaca ggtcaataaa gatggcaccg
     5701 actgaaagcg caataaaaac aatgaccgtt tcagtcggga agccgatgtg tgtggaaacc
     5761 atattttacc cttagggatg tggatcagag gccaaattca gccgggtcga agcccaacgc
     5821 gatagcgatc tcgcgggagg ccagtttctc gtcctgatcg aagttaccgt cacttttcgc
     5881 aacggcaata ccaacacgta aggccagctg agcggcctca ggctgatctt tcagcgccag
     5941 gatgtatttc atggtttcgc ccttgccaat ttcaacatcg aaatcgaagc ttgaaaccag
     6001 tttattgaag aactcaatca cctcattggt atcgaagacc ttcagctctt ctgaagagcg
     6061 cagaaagccc atcattttct gcttttcttc cgaacttacg ccgtcactcg atacggcaat
     6121 acgggcacat acagcaacgg tgccctgcat gaattttttg tttttgaaac ggccaacctg
     6181 gcgggtcagt tcgtcacggc ctgagttaat ggcaccttta actttgtcga aaaagctcat
     6241 acctgtttcc tttgttgctg tgttatttag aaccggcgct ccagcgaaat ccccagccaa
     6301 atgcccggtc catttcgtcc tggcctttga agtactggtt aattcgctcg actttaattg
     6361 cgccgttttc gtttacgagt ctggcaatgg cgcacaatgt gcgacggttc tcaccttcgg
     6421 tcaggcgggt ctcaatgggc ggttgatccg gcacatgaat ggtgactaca ccatcagtct
     6481 tgtcccagct gggaacacct tcataaataa aggcgtaaat cagcacttcg cggatatgct
     6541 tccattcacg tccattgata tgcagccact cgccgtctga tacatcaccc gtccggtcat
     6601 cgcccttgag ctgaacatac ggttcatcgc gataatcacc gaatgcatta ccgagcgcct
     6661 gaatgaccga tttatacccg tcctgaagct caacaaaggc ccccagatcc aggtcgattc
     6721 ctttggagcc gaacatacct gcaaagcctg atttgctact gccccggtgc cagttcaggt
     6781 tgatccggat ttcaccgaag ttatcccgtt tggtcaggct gattgcaggc ttctcttttg
     6841 tcagcgatac cttgctcagg ctgatttttg tctcaactgg cggaggggtt acaaccggag
     6901 gagttggggc tgcgggagcc ggttcgtcgg caatatttac gccgaaatgc tctgccagcg
     6961 gtttaagtcc accgttgaac ccctgcgcaa cgaagcggaa tttccagtca ttattacgac
     7021 ggtaaaattc ccccagaatt aaggctgctt cctgacggcc gctcagttcg acactgccgc
     7081 taaccaggcc ggtagcccct tgctccacgt cgatggaaag attacgaaga ccggacaccg
     7141 tctgcccacc gtcacaggtc acggtgaacg caattttctg gacatcaggt ttcagccggt
     7201 tgagcgcgac agtaaaggtt gagtactggc cttcactgac caggctgacg gtaccatcat
     7261 cgttacgtgg ctgaccataa aacaccatgt ccgcatcacc ctgcaccttc ccgtcggcat
     7321 acaggcgaaa cgccgaagca tccacctggc cgcccgaggt gatccggacc cgcaactcct
     7381 gggctggaac gggggcattg ccccccggcg tcaggttcat tagcgaataa ccgctgaaac
     7441 gatatcggag tgcatatcgt caattgtgcg gccgcgacag gcatggccaa gggcggtaaa
     7501 gtcccagttg ccgttattac ggcgcagcga agaaataacg atcccggtat gtgagccctg
     7561 ctcagtcagc ttgtagcgag ccagctcttt gccggtttga tcgacaacgc ggcagaaagc
     7621 gttttcgacg tcattgaacg actgaccacg gaagctgttt accgtaaacg ccaggtattc
     7681 aacgtttgca ggcaggcggg agaggttgac cttaatcact tcatcgtcac cgtcaccctc
     7741 accggtcagg ttatcgccac tgtgcacaac agcgccgcag gtggatttca gtttgcggaa
     7801 ccagatggtg tcaattgttt tacctgtgct gtccattaat acacagccag catcgagatc
     7861 gattgagtcg ttgcctccaa acaatcctcc aagaagacct tttttcttaa ccggatccca
     7921 accgagaccg aaatgaagct ggcttaatgc agatgattct ttgctgagtg ataccgtctg
     7981 gttcttaact aatgaaacca tgttttattc ct
//
LOCUS       DAZFWP020000200         8001 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFWP020000200 DAZFWP020000000
VERSION     DAZFWP020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.201"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(535..675)
                     /locus_tag="ACHK0Z_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             complement(535..675)
                     /locus_tag="ACHK0Z_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4504382.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(977..1240)
                     /locus_tag="ACHK0Z_RS22430"
                     /old_locus_tag="pgaptmp_004486"
     CDS             complement(977..1240)
                     /locus_tag="ACHK0Z_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4504383.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1663..1788
                     /locus_tag="ACHK0Z_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /pseudo
     CDS             1663..1788
                     /locus_tag="ACHK0Z_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            1853..2113
                     /locus_tag="ACHK0Z_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /pseudo
     CDS             1853..2113
                     /locus_tag="ACHK0Z_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2163..2402
                     /locus_tag="ACHK0Z_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /pseudo
     CDS             2163..2402
                     /locus_tag="ACHK0Z_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2450..3067)
                     /gene="ureG"
                     /locus_tag="ACHK0Z_RS22450"
                     /old_locus_tag="pgaptmp_004490"
     CDS             complement(2450..3067)
                     /gene="ureG"
                     /locus_tag="ACHK0Z_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGU4504384.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3079..3753)
                     /locus_tag="ACHK0Z_RS22455"
                     /old_locus_tag="pgaptmp_004491"
     CDS             complement(3079..3753)
                     /locus_tag="ACHK0Z_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HGU4504385.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(3754..4218)
                     /gene="ureE"
                     /locus_tag="ACHK0Z_RS22460"
                     /old_locus_tag="pgaptmp_004492"
     CDS             complement(3754..4218)
                     /gene="ureE"
                     /locus_tag="ACHK0Z_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGU4504386.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4228..5898)
                     /gene="ureC"
                     /locus_tag="ACHK0Z_RS22465"
                     /old_locus_tag="pgaptmp_004493"
     CDS             complement(4228..5898)
                     /gene="ureC"
                     /locus_tag="ACHK0Z_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGU4504387.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(5924..6244)
                     /locus_tag="ACHK0Z_RS22470"
                     /old_locus_tag="pgaptmp_004494"
     CDS             complement(5924..6244)
                     /locus_tag="ACHK0Z_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGU4504388.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6253..6555)
                     /locus_tag="ACHK0Z_RS22475"
                     /old_locus_tag="pgaptmp_004495"
     CDS             complement(6253..6555)
                     /locus_tag="ACHK0Z_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGU4504389.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(6565..7344)
                     /locus_tag="ACHK0Z_RS22480"
                     /old_locus_tag="pgaptmp_004496"
     CDS             complement(6565..7344)
                     /locus_tag="ACHK0Z_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGU4504390.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            7725..8000
                     /locus_tag="ACHK0Z_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             7725..8000
                     /locus_tag="ACHK0Z_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504391.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1934 a         2044 c         2029 g         1994 t
ORIGIN      
        1 tcgttttatt ctttcacttt tatcaaaaca tcaccagaaa ctggaagtga caaccgcaat
       61 aatgaacata aaaaggcaaa tcagaatggc agtaaatcaa taaagtacca tttcatatga
      121 atcatcagaa aaacggtgga cgcgacatcc agcacgaggc aaaacacagc aacaaaataa
      181 gccggatatc gcgccctgta cttttctaac tcaactcccg gcattgtgca agttttaatc
      241 gcaggtggat tcagagccac gcccccattg attcagcctg aagacacgta tatccttgga
      301 gaaagcagga atctcattgc tgtgaaattc cttacagaat cttcagcccc attttatggg
      361 gctttttttg gtgcccccac gctgttacca cactttcggg atgcgaataa aaaagcccac
      421 agtttttaca ctgcggactt ttgttttttc tttgtgattt gtgtgcaagg gtgcgacttt
      481 cgaccgaacc tttggcaacc ggatagcggg gactgttgtc cccgctgcgg tttcttacgg
      541 tttacactgc aagaacgccg caatttccga ccacatgaag cttaccctga attcacacgg
      601 tgatgcgtgt aacacccaga cggtcagcaa catggtcagg cataacatgc ccacaatgat
      661 cgttttttgc ggcatagcac ttgctccttt tacagagagg cgctaacctt ccacttgctg
      721 aaggtagatg gtcagggcct cgggttaacg tttaagttga ctcggggcct ttccacatcc
      781 ggccttcagg taatccttcc agccgtcagc cgaaaggcac ccgcgcgtaa tgtactggtt
      841 ttcagagagg tccgcaatct tccggtgacg agatcatctg cacacgcccc ggtgggacgg
      901 gctaacggta tattcaggtg caggatctct ctgtctggtg cgtttttaaa ttgtcaaacg
      961 caccctggcg gcaaaatcag ccaccttttt tcgcatcaac aaaacgccca aaacgctggg
     1021 taaagcgtgc aacattacct tctgaagcga cagttctcag tttacctgta taaaacggat
     1081 gcgacttaga agaaacgtca attgtaacat atgggtaagt tacaccatcc agttcaatta
     1141 cccggtcagt tttgatcgtc gaaccgatcc tgaagtactc atcaatactg gtatcgtgaa
     1201 atactactgt acggtattcc ggatggatat tcggtttcac aacagttttc cccctttaag
     1261 tgaaaattcc gcataaaggt acatattcag acggtcagat tcaactgtct gttcttataa
     1321 cctcactctc tttcagtatt acaatatgtt atataattat aaactggact tcccgaaaaa
     1381 atccattaaa taagaagcga gccgttgcgg aggcttgctc tctcctttaa cctcatgtgc
     1441 aatatccgta acgcgctcac ataaatcatc atctgataat ataacaacac ccattatttt
     1501 attctgacct aaaaacttct tatgaaataa acgtttaccc gacgtatctg atcccgctgt
     1561 ttttacctgt atcctttaac atacttgaga agcccgctga aaaatatacc gtaaaggcgg
     1621 ttctctgtct tccataaacc tgtcggataa gaagggcatt aaatgaaaac caaaaaagtt
     1681 atgatgattg cactggtcag ctctgcgctg gctctttcag gatgtggagc catgggcaca
     1741 gcgatcaaaa aacgaaatct ggaagtaaaa acccagatga gtgaaacctg aggtagcctg
     1801 agtttaacgg acactccttc ctgaaataga atggcatcag aaggagctaa taatgagcag
     1861 aaaaacccaa cgttactcta aagagttcaa agccgaagct gtcagaacgg ttcttgaaaa
     1921 tcaactttcg atcagtgaag gcgcttcccg attatctctt cctgaaggca ctttaggaca
     1981 atgggttacc gccgccagaa aagggctcgg tactcctggt tcccgcacgg tggctgaact
     2041 ggaatctgaa attctgcaac tgcgtaaggc gttaaatgaa gctcgccttg agcgagatat
     2101 attaaaaaaa gcagctgtag attcaatctg tcaatgcaac acccctttca attatctctt
     2161 tcactgcccg cgacggtatc ggttatcagt ttgacctgct ggctaacggt gaaaccgtac
     2221 tgcgtgatgc atccggcgaa aaacgcatca agggtgaaaa agacggtcgt atgctcatga
     2281 atgttggcct gaatgcggaa attcgcgaca acatccgctt cggtctggag tttgaaaaat
     2341 cggcatctgg taagtacaac gtggataacg cggtgaacgc caacttccgt tattccttct
     2401 gataaaaaca aggggccgcc tgccggccct gatacagagg atgttgtgat tacttaccca
     2461 gcatgccctg ctcttcgata aaggcgatga tgttttgcag accttcacca cttttcaggt
     2521 tagtgaaccc ccatgggcgt tctccgcgca tacgtaaagt atcacgctcc attacctcga
     2581 gggaggcgcc cacgtatggt gcaaggtcag ttttattgat cacgaggaag tcggatttgg
     2641 tgatccccgg accgccttta cgcgggatct tttctccttc tgccacatct atgacatata
     2701 tggtaaggtc cgccagttcg gggctgaagg tcgcactcag gttgtcgccg ccgctttcca
     2761 caaagatcag atcgagatta ccaaatttct cactcagtgc ttccacggct gccagattca
     2821 ttgatgcgtc ttcacgaatg gctgtatgag ggcagccccc cgtttcgaca ccgacaatgc
     2881 gttccgcttc cagtgctccg gcctcagtga gaatgcgttg atcttctctg gtgtagatgt
     2941 cgttggtgac tacggctaat tgccaggtgt cacgcatgga tttgcagagg gcttccagaa
     3001 gcgcggtttt acctgagccg accggaccgc cgacgccgac acgcagcggt tgcttgatta
     3061 tgttcatttt attttctcct aagaacggaa taatcgggaa tactgggttt catgtcgcgc
     3121 ggaagcgata gatgcaagtg gcgttgctga accaattgcg tgatccgggg ttgcaagggc
     3181 actgtccatg ttcgccgcat agcgggcaca caggcgcata attaactgct gggcagccag
     3241 ctggccgtag ggtaccagcc tgacgcccgc catcactgcg ctttcaatcc agctgtagcc
     3301 aaggctcagg gccaggtctg aaacggatat ttgccagcgt acgccgagcc atgccatacc
     3361 ggttaactgg ctttgctggc atagtttgcg ccattcggcc gggcagtcgg gctgccagtc
     3421 gaccagaagt ctggtgaagg ctgcaccgcg gttacgttct tcatcgcgta gctcacgggt
     3481 ttccctgcag gccagcagat aagcggtcca gcgcccggca ctgtcaggat cgcccgcttc
     3541 acaggcccgg tatagccggg caagcagtgg caggtcgacg gcaaaaaaac tctgttccat
     3601 ctgcaactgc tgccagtgtt caaaggcggc gctatcttct acccagcctg cttccactgc
     3661 ccactccagt ccctgggacc agctgtagcc tccaacaggg aggctgttac tggcaagctg
     3721 catcagccgc agacgttttt ccggagtcgg cattcagtgg ctgtgctcct gatcgtgatt
     3781 atgggcgtgc gacttgctgg cgtaagcgcc agcttccggc tcaaacggaa gatgtgcaaa
     3841 atcaacatcc agcccaaact ggcgcagcat atcatcgagg acgtgatcgt gatggtagcg
     3901 cagctctccg ggcattattt gcagcggaac gtggcgattg ccgagatgcc agcaggcttt
     3961 cgcgagcata aaaggatctg cgcagcgcac tacagaaacg gcttcatcag cggcgatcac
     4021 cttaatgaat tcatcaccgt tttcgttact gagaatatca ccatcgcgca gcagcagacc
     4081 gcgtggcagc aacagtccag cctgacgtcc atcgttaagg gtgactttaa tacggctttt
     4141 tacccgcatg tcaacaggga gggtaacgct ggcagttgtc tgagccgggg tctccacccg
     4201 ccgggtcaaa taaagcattt gtgctcctta aaacaggaaa taacgttgcg ccattggcag
     4261 aatgtccgcc ggttcactgg ttatcagttc gccgttgata cgcacttcgt aggtttgtga
     4321 atccacggtt atatcaggca acaggctgtt gtggcgcata tcggcttttt gtactgtgcg
     4381 gcagcctttt accacagcag ttgtgctgtg caggttaagc tgttcagcga cgccatttgc
     4441 tgctgctgcc tgcgacagga aagtcacacg acagcggtga cgggcactgc ccaatgcagc
     4501 gaacattggg cgatagtgca ccggctgcgg tgtggggata gagccgttga tatcacccat
     4561 cggcgccatg gctatcattc cgcctttgac gatagtcgcc ggttttacgc caaagaacgc
     4621 cggggaccag agcaccagat ccgccagttt tcccacttca atcgagccga cttcatgagc
     4681 aataccgtgg gttaatgccg gattaatggt gtatttagcg atatagcgct tcacgcggac
     4741 gttgtcgtta tcaccacttt cttccggtaa cgggccgcgc tgaactttca tccggtgtgc
     4801 cacctgccag gttcgtaaca ctacttctcc gacgcgtccc atggcctgcg aatcggacga
     4861 ggtgagggag aacgcgccaa ggtcgtgcag gacgtcttcc gcggcaatgg tttcctggcg
     4921 aatgcgcgat tcggcaaagg ctacgtcctc ggcgatatcc gggtcaagat gatggcaaac
     4981 catcagcatg tccagatgct catcaatagt gttgacggta tagggtagcg tcggattggt
     5041 tgaggaaggc agaatattgg ggtgcgcgca ggcggtgata atatccggag catgaccacc
     5101 acctgcacct tctgtatgga aggtatggat agtgcgcccg ccgatggcag tcagagtatc
     5161 ctcaacgaat cctgattcgt taagcgtatc actgtgtagc gcaacctgaa cgtccatttc
     5221 gtcggctaca gtcagtgcgc agttgattac cgcaggtgta gctccccagt cttcgtgaat
     5281 tttgaggccg ataaccccgg ccgcgacctg ctcacgaagc gcatccggat tggagcaatt
     5341 gcctttaccc agcaacccga tattgaccgg caggctgtct gcagcctgca gcatttgata
     5401 aatgtaccat ggtcctgggg tacaggttgt ggcgttagaa cccgctgtcg ggccagtacc
     5461 gccaccgatc atggtggtaa tgccggatgt cagcgcttct tcagcctgct gtgggcagat
     5521 ccagtgaata tgcgtatcga cgccacctgc ggtaacgatc ctgccttctg cggcaataat
     5581 ttccgtggat acgccgattg ggatagtgac gttgggttgt atatcaggat taccggcttt
     5641 gccgatagca aatatccttc catctttgac gccgatatcg gctttaacga tcccccagta
     5701 atcgatgatc agggcattgg tcagcaccag atcagcgcag ccggcggaga gcatttgccc
     5761 ctgtcccata ccgtcgcgga ttactttacc gccgccgaat ttgacctctt cgccgtaggt
     5821 agttaaatca tcttcgacct cgatccacag ctcagtgtct gccagacgaa ttttatcacc
     5881 ggtggtaggg ccgaacatgt cagcataggc ctagcgtgaa atattactca tcatttgcct
     5941 ccagctcacc cattatatct ccgcggaaac cgtgcacgat acgggcaccg ctcagcgcca
     6001 ccagatcaac ctcacgcttt tggcctggtt cgaagcgcac tgcagttccg gcggcgatat
     6061 tgagacgata gcctcttgcc ttctggcgat cgaacttcag tgccgggtta acctccgcga
     6121 agtgataatg ggaaccgacc tgaattggtc ggtctccgtg attttcaacg ataatactgc
     6181 aggtagcccg gccgatgtta agctctatat agccaggttt aaccttatat tctccaggga
     6241 tcatctgtta cctcagatta tcggattatg gacggtaacc agcttggagc cgtccggaaa
     6301 ggtggcttcg acctggatat cggggatcat ttctggtatg ccttccatga cctgctcgcg
     6361 actcaggaca tgccgtcctt cttccatcag cgcagcgacg cttttgccgt cgcgagcgcc
     6421 ctccattata aaagcgctaa tcagggccac ggattcggga tagttaagtt tcaggccgcg
     6481 ggccagacga cgctctgcca gcagtgcagc ggtaaacagt aatagtttgt ctttttctct
     6541 tggagtcagt tccatagcgt tctcttaagt cagccagatc cggggaagta ccggtgattt
     6601 gtcagtcagt tgcggccgca taaattgcca gatatcacgc attacctgct ggcaacgcag
     6661 attatcatca cttaaaaagc gaactgtcag tagaccatcg gtgagcgtgg ctccggcgta
     6721 gttttccagt ggtgtgagtt tttcgcgtat gtcttccagt tgcgtttcat tccccaggta
     6781 gaacagcatc gttcccaccc atggtttccg ggcaacgcag gtaagttctc cattggccac
     6841 gtgcagacgc tcaatcaaca gcggagaacc gtcgacccat atctccagac ggttagcgag
     6901 tgttccgtgg ctgaaggttt ctccgatgac cggacgtcca aggcacagaa gatcccaggc
     6961 cagcagtgta ctggaggacg acagatgaaa cacggtatgc aacgtggcgt gagcgccggg
     7021 aaaaaaaatc gaatcctgtg gcaaccactc cagcgttgcg ttattgcaca gcgtcaggtt
     7081 ttgctgaagg tgcgcctgcg cgccgctgct gcggtaaaat ttactggcac caggcatggt
     7141 gatcagcgta tggcaatcag agtcaattat tgcgttgatg atgagttcgt caccgccgac
     7201 gataccaccg ggtggatgaa gcagacagag gtggcaggtt tcttcttcgg gataaaaagg
     7261 acgctgaaca gtgagcggac cgacatgatg acgggtggtg agcgtggttt tcccgtgcag
     7321 acgctgaaaa cggagatcca gcatggcctg ccagcctttt ttattttgtg gtaacgcggt
     7381 actgagcaat cttacgcccc tgataaataa agataaaacg acagtaaaga gccggatttc
     7441 cttgttatat aaaagatgat gcctttccag tgagttttcg tctgaataat acgaatgttt
     7501 gttttcgtca tgaagcgttc cggctcccgg ggctatacat cgccagaaaa gcaatgcata
     7561 ttgtcgggat tatccagaga atcaggcaga aagatccgac aatgggctgt gattatgcca
     7621 tctggctcag aatgatatca aggtgttatg gggataactt gctgatacaa tagcagtatg
     7681 acagattcat taatgcggaa actgctcact gaaacagagt gttcatgact gagcactggt
     7741 cgggtcatgc agatgataat ttcccctttt cttggggaag cgacagtatt ttcatgcatg
     7801 cctgtcttca gtcatacggg gagagcatct gtgatacagt ccatacattt gttgcggacg
     7861 tcgctcctga gtcactgact ggtctgcaaa cagtttatct gtacacggca gggcttctgt
     7921 gtgatatcgt ttttcatatc gagggggggc ctccagagat gaatggaata ataatatttc
     7981 ctgatgatat aataccttag a
//
LOCUS       DAZFWP020000201         7909 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFWP020000201 DAZFWP020000000
VERSION     DAZFWP020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.202"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            143..232
                     /gene="kdpF"
                     /locus_tag="ACHK0Z_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             143..232
                     /gene="kdpF"
                     /locus_tag="ACHK0Z_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU4504392.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            232..1905
                     /gene="kdpA"
                     /locus_tag="ACHK0Z_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             232..1905
                     /gene="kdpA"
                     /locus_tag="ACHK0Z_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU4504393.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1928..3976
                     /gene="kdpB"
                     /locus_tag="ACHK0Z_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             1928..3976
                     /gene="kdpB"
                     /locus_tag="ACHK0Z_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU4504394.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3985..4557
                     /gene="kdpC"
                     /locus_tag="ACHK0Z_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             3985..4557
                     /gene="kdpC"
                     /locus_tag="ACHK0Z_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU4504395.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4550..7234
                     /gene="kdpD"
                     /locus_tag="ACHK0Z_RS22510"
                     /old_locus_tag="pgaptmp_004502"
     CDS             4550..7234
                     /gene="kdpD"
                     /locus_tag="ACHK0Z_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU4504396.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSYDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7231..7908
                     /gene="kdpE"
                     /locus_tag="ACHK0Z_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             7231..7908
                     /gene="kdpE"
                     /locus_tag="ACHK0Z_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU4504397.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1609 a         2068 c         2360 g         1872 t
ORIGIN      
        1 taaattaata ctaaacatta gttaaatcat ggcttttgcc atttttatac tttttttaca
       61 ccccgcccgc agatttttgc gaaatctttg cagccagaat tctacccttc cggtatcact
      121 tttaggccac tggaggtgca ctgtgagtgc aggcgtgata accggcgtat tgctggtgtt
      181 tttattactg ggttatctgg tttatgccct gatcaatgcg gaggcgttct gatggctgcg
      241 caagggttct tactgatcgc cacgttttta ctggtgttaa tggtgctggc gcgtccttta
      301 ggcagcgggc tggcgcggct gattaatgac attcctcttc caggtacagc gggcgttgaa
      361 cgtatactct ttcgtttacc tggcgtctct gaccatgaga tgaactggaa gcaatatctt
      421 tgtgccattc tcggcctgaa catgctgggg ctggcggtgc tgttttttat gttgctcggt
      481 cagcactatc tgccgcttaa tccacagcag ttgccggggc tgtcgtggga tctggcgctg
      541 aataccgccg tcagctttgt cacgaatacc aactggcaat cttacagcgg tgaaaccacg
      601 ttgagctatt tcagccagat ggctggctta acggtgcaaa actttctttc tgccgccagc
      661 gggattgcgg tgatttttgc cctcatccgt gcgtttaccc gccagagcat gagcacactc
      721 gggaatgcct gggtcgatct gctacgcatc acgttatggg tgctcgtccc tgtggcgttg
      781 ttgatcgcac tgttttttat tcaacaaggt gcgctgcaaa actttcagcc ttatcaggct
      841 gtgaataccg ttgaaggagc gcaacagctg ttacccatgg ggcctgtagc ttctcaggaa
      901 gcgatcaaga tgctcggtac taacggcggt ggcttcttta atgccaactc gtcgcatccg
      961 tttgaaaacc caaccgcact gaccaacttc gtgcagatgc tggcgatctt cttgatccca
     1021 acggcgctgt gctttgcctt tggtgaagtg acgggcgatc gccgccaggg gcgcatgttg
     1081 ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg tggtgatgtg ggcagaagtt
     1141 cagggtaatc ctcatctgct ggcgctcggc gcagacagca gcatcaatat ggaaggtaaa
     1201 gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac ggcggcttcc
     1261 tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc tcggtggcat ggtgccgatg
     1321 tggctgatgc aaattggtga agtggtgttc ggcggtgtcg gttctggtct ttacggcatg
     1381 atgctgtttg tcctgctggc ggtgtttatt gccgggctga tgattggtcg tacaccggaa
     1441 tatctgggta aaaaaatcga cgtacgcgag atgaaactga ctgcactggc gattctggtt
     1501 accccgacgc tggtgctgat gggcgcggcg ctggcaatga tgaccgacgc cggacgtagc
     1561 gccatgctca accctggccc gcatggtttt agcgaagtgc tgtacgccgt gtcatccgcc
     1621 gctaacaaca acggcagcgc ctttgccgga ttaagcgcca actccccgtt ctggaactgt
     1681 ttactggcgt tctgcatgtt tgtcggtcgc ttcggggtga ttatcccggt gatggcaatt
     1741 gccggttcgc tggtgagtaa aaagagccaa cccgccagct ccggcacgct gccaacgcac
     1801 ggcccgctgt ttgttggcct gttaatcggc accgtgttgc tggttggcgc actgaccttt
     1861 atccctgccc tggcgcttgg tccggtggcg gaatatctct cctgatgata ttgagtgagc
     1921 actgaatatg agtcgtaaac aactggcgct attcgaacca acacttgtcg ttcaggcgct
     1981 gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg tgatgtttat
     2041 cgtctggatc ggcagtctgc tgaccacctg tattagcatc gcgatggcaa gcggtgcgat
     2101 gcctggcaat gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca ccgtactgtt
     2161 cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa gcgcaggcca acagtcttaa
     2221 aggggtgaaa aaaactgcct ttgcccgcaa gctgcgtgag ccgaaatatg gcgctgcggc
     2281 ggacaaagtt cctgccgatc aactgcgtaa aggtgacatc gtgctggtag aagctggcga
     2341 tattatcccc tgcgatggtg aagtcattga aggcggcgca tcggtcgatg aaagcgccat
     2401 caccggggaa tcggcaccgg tgatccgtga atccggcggc gattttgcct ccgtcaccgg
     2461 cggcacgcgt attctttctg actggctggt gattgagtgt agcgttaacc ccggtgagac
     2521 atttctggat cggatgatcg cgatggtgga aggcgcacag cgacgcaaaa caccgaacga
     2581 gattgccctg actatcctgc tgattgctct gaccatcgtc tttttactgg caaccgccac
     2641 gctgtggccg ttttccgcgt ggggcggtaa tgccgtgagt gtaacggtac tggtggcgct
     2701 gctggtctgt ctgatcccaa ccactattgg cggcctgttg tcagcgatcg gcgtcgccgg
     2761 gatgagtcgg atgctgggcg cgaatgtgat tgccaccagc ggacgtgcag ttgaagcggc
     2821 aggtgacgtt gacgttctgc tactggataa aaccggcacc atcacactcg gtaaccgtca
     2881 ggcgtcggag tttatccctg cgcagggcgt ggatgaaaaa acgctggctg acgccgcaca
     2941 actggcttcg ctggctgatg aaacgccgga aggccgcagt attgtgatcc tcgccaagca
     3001 acgttttaac ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg taccgtttac
     3061 tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc atgatccgta aaggttctgt
     3121 cgatgccatt cgccgccatg ttgaggctaa cggcggtcac ttccctaccg atgttgatca
     3181 aaaagtcgat caggttgcgc gtcagggggc cacgccgctg gtggtggtgg aaggttcccg
     3241 tgtgctgggc gttattgcgc tgaaagatat cgtcaaaggc ggtattaaag agcgcttcgc
     3301 ccagctgcgc aaaatgggca ttaaaacggt gatgattacc ggcgataacc gtctgactgc
     3361 cgccgccatt gctgcggaag cgggcgtcga tgattttctc gccgaagcga caccggaggc
     3421 caagctggca ttgattcgtc agtatcaggc ggaaggccgt ttggtggcga tgaccggcga
     3481 cggcaccaac gatgctccag cgctggcgca ggcagatgtc gcggtggcga tgaactccgg
     3541 tactcaggcg gcgaaagaag cgggcaatat ggtcgatctc gactctaacc cgaccaagtt
     3601 gatcgaggtg gtacatattg gcaaacagat gctgatgacc cgtggctcgc tgaccacctt
     3661 cagcattgcc aacgatgtgg cgaaatactt cgccattatt ccggcggcgt tcgcggcaac
     3721 gtatccgcag ttaaatgcgc tgaacatcat gcgccttcat tcgcccgact ccgccattct
     3781 cagtgcggtc attttcaacg ccttgattat cgtctttttg attcctctgg cgttaaaagg
     3841 cgtgagttat aaaccgctta ccgcttctgc catgttgcgc cgtaacttat ggatttacgg
     3901 tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc attgatttac tgctgaccgt
     3961 ttgcggtctg gtgtgaggtt taccatgcgt ggcttacgtc cggcattatc gacatttatc
     4021 tttctgttat tgattactgg cggcgtttac ccgctgctga ccaccgtgct ggggcaatgg
     4081 tggtttccct ggcaggccaa cggttcgttg attcgtgaag gtgatacggt gcggggttcg
     4141 gcattaatcg ggcagaattt taccggcaac ggctattttc agggtcgccc gtcggcaacg
     4201 gcagaaatgc cctataatcc acaggcttct ggcgggagca atctggcggt cagtaaccct
     4261 gagctggata aacaaatagc cgcacgcgtt gctgcattac gggccgctaa cccggatgcc
     4321 agtacgaacg ttccggttga actggtgacg gcatcggcaa gcgggctgga caataatatc
     4381 accccgcaag cggcggtctg gcaaatccca cgcgtggcga aagcgcgtaa tctcagcgtt
     4441 gaacagctca cgcaactgat cgcaaaatac agccaacaac cgctggtgaa atatatcggc
     4501 cagccggtcg tcaacattgt tgaactcaat ctggcgctgg ataaacttga tgaataacga
     4561 acccttacgt cccgaccccg atcgtctgct ggaacaaact gccgcgccgc atcgggggaa
     4621 gctgaaagtt ttcttcggtg cctgtgcagg cgtcgggaag acctgggcga tgctggcaga
     4681 agcccagcga ctgcgggcgc aagggctgga tattgtggtt ggcgtggtag aaacccatgg
     4741 gcggaaagat accgccgcca tgcttgaagg gctggctgtt ctgccgccca aacgccaggc
     4801 gtaccgtggg cggcatatca gcgagtttga tctcgatgcc gccctcgccc gccgtccggc
     4861 gctgatctta atggacgaac tggcgcacag taatgcgcca ggttctcgtc atcctaaacg
     4921 ctggcaggat atcgaagaac tgctggaagc tggcattgat gttttcacaa ccgtcaacgt
     4981 tcagcatctg gaaagtctga atgatgtggt cagcggcgtc accggaattc aggtacggga
     5041 aaccgtgccc gatccttttt tcgatgccgc cgacgacgta gtgctggtgg acttgccccc
     5101 ggacgatctg cgccagcggc tgaaagaagg caaagtctat attgccgggc aggcggagcg
     5161 cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc ctgcgtgaac tggcactgcg
     5221 ccgtactgcc gatcgcgtag atgagcaaat gcgcgcctgg cgggggcatc ctggtgaaga
     5281 gaaagtgtgg cacacgcgcg acgcgatcct tttatgcatc gggcataaca ccggcagtga
     5341 gaaactggtc cgcgcggcgg cgcggttggc ctcgcgcctg ggtagcgtct ggcacgcggt
     5401 gtatgttgaa acccctgccc tgcatcgctt accggaaaaa aaacgtcggg caattctcag
     5461 cgccttacgt ctggcgcagg agctgggcgc ggagacggct acactttctg atccagcgga
     5521 agaaaaagca gtagtgcgtt atgcccgtga acataatctc ggcaagatta ttctcggtcg
     5581 cccggcctcg cgccgctggt ggcgtcggga aacgtttgct gaccgactgg cgcgcatcgc
     5641 ccccgatctc gatcaggtgc tggtcgcgct tgatgaacca cccgcccgca cgattaacaa
     5701 cgcgccggat agccgctctt ttaaagacaa gtggcgggta cagattcagg gatgcgtggt
     5761 tgccgccgcg ttatgcgccg ttatcacttt aattgccatg cagtggctga tggcgtttga
     5821 tgccgccaac ctggtgatgc tgtatctgct tggcgtggtg gtggtggcgc tattttatgg
     5881 acgctggcct tcggtggttg ctaccgtcat taatgtggtg agttacgatc tcttttttat
     5941 cgccccacgc ggcacgctcg ctgtctctga tgtgcaatat ctgctgacct tcgcggtgat
     6001 gttaaccgtc gggctggtga tcgggaacct tactgcgggt gtgcgttatc aggcgcgggt
     6061 agcccgttac cgcgagcaac gcacacggca cttatatgaa atgtcgaaag ctctggcggt
     6121 gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa tttattgcct ccacgtttca
     6181 tgcccgcagt caggtgttgt tgcccgatga caacggtaaa ttgcagccgt taacacatcc
     6241 gcaaggaatg acgccatggg acgatgccat cgcgcagtgg agttttgata aaggcctgcc
     6301 tgcgggtgcg ggcaccgaca cgttacccgg cgtgccgtac cagattttgc cgctaaaaag
     6361 cggcgagaaa acctacggac tggtggtggt ggaaccgggg aatctgcgtc agttgatgat
     6421 cccggagcag cagcgcctgc tagagacgtt tacgctgtta gtcgccaatg cgtttgagcg
     6481 gctgacgctg accgccagtg aagaacaggc gcggatggca agcgaacgtg aacagatccg
     6541 caacgccctg ctggcggcgc tttcgcatga tttacgcacg ccgcttacgg tgctgtttgg
     6601 tcaggcagaa atcttaacgc tcgatctggc aagcgaagga tcgccccacg cccgccaggc
     6661 cagcgagatt cgtcagcatg tgctgaacac tacccgactg gtgaataatc tactggatat
     6721 ggcgcgaatt cagtccggcg gctttaattt gaagaaagag tggttaacgc tggaagaagt
     6781 agtcggcagc gcgctgcaaa tgctggaacc gggtttatcg tcgcccatca atctttctct
     6841 gccagaaccg ctgaccttaa tccacgttga cgggccactc tttgaacggg tgctgattaa
     6901 tctgctggag aacgcggtga aatatgcggg tgcgcaggcc gaaattggta tcgatgccca
     6961 cgttgagggc gaaaatctac aactggatgt ctgggataac ggccccggtc ttccgccagg
     7021 ccaggagcag acgatatttg ataagtttgc tcgcgggaat aaagagtcgg cagtaccggg
     7081 ggtagggctt ggactggcaa tttgtcgggc gatagtggat gtacacgggg gcactattac
     7141 cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc agcaaaccgc
     7201 tcctgaactt gaagactttc atgaggatat gtgacaaacg ttctgattgt tgaagatgaa
     7261 caggctattc gtcgctttct gcgcacggcg ctggagggcg acgggatgcg cgtctatgag
     7321 gccgaaacgc tgcaacgcgg cttgctggaa gcggctaccc gtaagccaga tttgattatt
     7381 ctcgatctcg gcctgcccga tggtgatggg attgagttta tccgcgacct gcgccagtgg
     7441 agcgcggtgc cggtgattgt gctttccgca cgcagcgaag agagcgacaa aatcgccgcg
     7501 ctggatgccg gagcggatga ttatctgagt aagccgtttg gcattggcga attgcaggcc
     7561 cgtctgcgcg tcgctttacg ccgccactcc gccaccgccg cgcccgatcc gctggtgaaa
     7621 ttttccgatg ttaccgtcga tttagccgcc cgcgtgattc accggggtga ggaagaggtg
     7681 catctcacac cgattgagtt ccgcctgctg gcggtgctgc tgaataatgc cgggaaagtg
     7741 ctcacccagc gccagttact caatcaggtg tgggggccaa acgcggtcga acacagtcac
     7801 tatttgcgta tttatatggg acatctgcga caaaaactgg aacaggatcc cgctcgcccc
     7861 cgccatttca ttactgaaac cggtattggg tatcggttta tgccatgaa
//
LOCUS       DAZFWP020000202         7816 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFWP020000202 DAZFWP020000000
VERSION     DAZFWP020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.203"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(209..472)
                     /gene="cedA"
                     /locus_tag="ACHK0Z_RS22520"
                     /old_locus_tag="pgaptmp_004504"
     CDS             complement(209..472)
                     /gene="cedA"
                     /locus_tag="ACHK0Z_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU4504398.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            655..2916
                     /gene="katE"
                     /locus_tag="ACHK0Z_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             655..2916
                     /gene="katE"
                     /locus_tag="ACHK0Z_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU4504399.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDSTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2963..3721)
                     /gene="chbG"
                     /locus_tag="ACHK0Z_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             complement(2963..3721)
                     /gene="chbG"
                     /locus_tag="ACHK0Z_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU4504400.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLNV"
     gene            complement(3734..5086)
                     /gene="celF"
                     /locus_tag="ACHK0Z_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /gene_synonym="chbF"
     CDS             complement(3734..5086)
                     /gene="celF"
                     /locus_tag="ACHK0Z_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4504401.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5191..6033)
                     /gene="chbR"
                     /locus_tag="ACHK0Z_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             complement(5191..6033)
                     /gene="chbR"
                     /locus_tag="ACHK0Z_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU4504402.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6041..6391)
                     /gene="chbA"
                     /locus_tag="ACHK0Z_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             complement(6041..6391)
                     /gene="chbA"
                     /locus_tag="ACHK0Z_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU4504403.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6442..7800)
                     /gene="chbC"
                     /locus_tag="ACHK0Z_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             complement(6442..7800)
                     /gene="chbC"
                     /locus_tag="ACHK0Z_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU4504404.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2119 a         2038 c         1731 g         1928 t
ORIGIN      
        1 caggatgaac tatcaaaaat agctcatcat gattagcaaa acttaaccat tttaaaataa
       61 ataaacaatt aaagaaaaaa gatcacttat ttatagcaat agctcgtcaa aggcagcttt
      121 ttgttacagg tggtttgaat gaatgtagca acgaaatgca gaatttcagg tcatgtaact
      181 cccggcaaaa ccgggaggta tgtaatcctt actcagtcac ttccccttcc tggcggatct
      241 gatttgccca acgttgggca gattcaggca cagtaaacgc cggtgagcgc agaaatgact
      301 ctcccatcag tacaaacgca acatatttgc cacgcagcat ccagacatca cgaaacgaat
      361 ccatctttat cgcatgttct ggcggcgcgg gttccgtgcg tgggacatag ctaataatct
      421 ggcggttttg ctggcggagc ggtttcttca ttactggctt cactaaacgc atattaaaaa
      481 ttagaaaaac tgtagtttag ccgatttagc ccctgtacgt cccgctttgc gtgtatttca
      541 taacaccgtt tccagaatag tctccgaagc gggatctggc tggtggtcta tagttagaga
      601 gttttttgac caaaacagcg gccctttcag taataaatta aggagacgag ttcaatgtcg
      661 caacataacg aaaagaaccc acatcagcac cagtcaccac tacacgattc cagcgaagcg
      721 aaaccgggga tggactcact ggcacctgag gacggatctc atcgtccagc ggctgaacca
      781 acaccgccag gtgcacaacc taccgcccct gggagcctga aagcccctga tacacgtaac
      841 gaaaaactta attctctgga agacgtacgc aaaggcagtg aaaattatgc gctgaccact
      901 aatcagggcg tgcgcatcgc cgatgatcaa aactcactgc gtgccggtaa ccgtggtcca
      961 acgctgctgg aagattttat tctgcgcgag aaaatcaccc actttgacca tgagcgcatc
     1021 ccggaacgta ttgttcatgc acgcggatca gcagctcacg gttatttcca gccatataaa
     1081 agcttaagcg atatcaccaa agcggatttc ctctcagatc cgaacaaaat caccccagta
     1141 tttgttaggt tctctaccgt tcagggtggc gctggctctg ccgataccgt gcgtgatatc
     1201 cgtggttttg ccaccaagtt ctatactgaa gagggtattt ttgacctcgt tggcaataac
     1261 acgccaatct tctttatcca ggatgcgcat aaattccccg attttgttca tgcggtaaaa
     1321 ccagaaccgc actgggcaat tccacaaggg caaagtgccc acgatacttt ctgggattat
     1381 gtttctctgc aacctgaaac tctgcacaac gtgatgtggg cgatgtcgga tcgcggcatc
     1441 ccccgcagtt accgcaccat ggaaggcttc ggtattcaca ccttccgcct gattaatgcc
     1501 gaagggaagg caacgtttgt acgtttccac tggaaaccac tggcaggtaa agcctcactc
     1561 gtttgggatg aagcacaaaa actcaccgga cgtgacccgg acttccaccg ccgcgagttg
     1621 tgggaagcca ttgaagcagg cgattttccg gaatacgaac tgggcttcca gttgattcct
     1681 gaagaagatg aattcaagtt cgacttcgat cttctcgatc caaccaaact gatcccggaa
     1741 gaactggtgc ccgttcagcg tgtcggcaaa atggtgctca atcgcaatcc ggataacttc
     1801 tttgccgaaa acgaacaagc agcattccat ccaggtcata ttgttcctgg tctggacttc
     1861 accaacgatc cgctgttgca gggacgtttg ttctcctata ccgatacaca aatcagtcgt
     1921 cttggtgggc cgaatttcca tgagattccg attaaccgtc cgacctgccc ttaccacaac
     1981 ttccagcgtg atggcatgca tcgtatgggg atcgacacta acccggcgaa ttacgaaccg
     2041 aactcgatca acgataactg gccgcgcgaa acaccgccgg gaccgaaacg cggcggtttt
     2101 gaatcatatc aggaacgcgt ggaaggaaat aaagttcgtg agcgcagtcc atcgtttggc
     2161 gaatattatt cccatccgcg tctgttctgg ctaagtcaga cgccattcga gcagcgccat
     2221 attgtcgatg gtttcagttt tgagttaagc aaagtcgttc gtccgtatat tcgtgagcgc
     2281 gttgttgacc agctggcgca tattgatctc actctggccc aggcggtggc gaaaaatctc
     2341 ggtatcgaac tgactgacga ccagctgaat atcaccccac ctccggacgt caacggtctg
     2401 aaaaaggatc catccttaag tttgtacgcc attcctgacg gtgatgtgaa aggtcgcgtg
     2461 gtagcgattt tacttaatga tgaagtgaga tcggcagacc ttctggccat tctcaaggcc
     2521 ctgaaggcca aaggcgttca tgccaaactg ctctactccc gaatgggtga agtgactgcg
     2581 gatgacagta cggtgttgcc tatagccgct acctttgccg gtgcaccttc gctgacggtc
     2641 gatgcggtca ttgtcccttg cggcaatatc gcggatatcg ctgacaacgg cgatgccaac
     2701 tactacctga tggaagccta caaacacctt aaaccgattg cgctggcggg agacgcgcgc
     2761 aagtttaaag caacaatcaa ggtcgctgac cagggtgaag aagggattgt ggaagctgac
     2821 agcgccgacg gtagttttat ggatgaactg ttaacgctga tggcagcaca ccgcgtgtgg
     2881 tcacgcattc ctaagattga caaaattccg gcgtaaatat ggataaggtc acgtcccctg
     2941 gggagtgacc ttaccacctt acttacacat taagataact acccaaccga taaccacgct
     3001 cagcaattgc atatttcaac gacgctgatg tcagcacttc cagctccgtt aaccgtggga
     3061 aacagtaagc actctgacgg atagtattat cgataaacgc cggatgacac atcacctcca
     3121 gcgaacgatc accccgatgg cttgcgtcat ccagcacttg caggaacagt gcctcgctaa
     3181 tctcttcacc ataaaacgcg ctactgaatc cctgagaact gcgcagatta gccggtaaat
     3241 caccagcgtt agatagcggc tgacggtcga tgcgcaacgc aatcccctct tcagccgcaa
     3301 acctggcaac aatcgggaaa atctgcggga acatatgcac atgatgatgg ctatcaagat
     3361 gcgtaggttt gcgtccaaat agctcaatga aacgcaaata ctgactggca agctcctgag
     3421 taatttcttc cagcggtaaa gcatcttctt ctgccaactg ccagatccat tttcccagca
     3481 caccatcgcg ggttaacccc ggcatagctg tcagtggctt gcccatagta aggacaaagt
     3541 gcatccctat ggccagactc ggttcatcac gactcaactg caccgcatgg tcaatagcct
     3601 gcccattcac cagcgccgtc gttgatgtga caatcccatt gcgacaggcc tcgataatgc
     3661 cgtagttctg acctttgctt aagccaaaat catcggcatt aacaatcagt aagcgttcca
     3721 taatcagcct cggttaatgt gcttttttaa gctctgcgat gcagtcggca aaatttggca
     3781 gccatttctc gtgcgccaga atcatctcgc gtgccagcag ctcagcatcg cgatcggaat
     3841 gcaccaacgg actaaggttt agcgccagta acacatcgtt aaattctccg ctaagtgcgg
     3901 cgttgctggc agcaatctcg aagcctttaa tggtgtgaat cagccccatc actttatcat
     3961 cgaaatgcgt aatgcgcgga tgtggcgtcg cgccatcgcg ccccagctta caggtcattt
     4021 ctaccgccca gtctgccgga atattatcaa tatgcccatg atgcgggata ttaacgtaat
     4081 gttcagcttg cttgtcgttg tagatagcgt tgatcacttc gcacgctgca tcagagtaat
     4141 aagccccacc gcgctgttcc agttctttcg gcttaacttt caactccgga tttttataca
     4201 gctcaaaaag ttgtttctcg actttctgta ctacctgtgc tcgtgcgccg cctttgtagt
     4261 attcgcccat ttcaatagcc agcatctctt tctgcttgaa gtaatacagc agataagaac
     4321 atggcagcag atttaacgaa cgaattaagc cctcactaaa tggcagatcg aaaatatttt
     4381 ttacagagga cgcttttaac tgccctgacg ccacaccatc aagcaattcg gcaaagcgcg
     4441 acttgccatt taccagcaca tccttaatga acaccatatg gttgaggccg aacagatcga
     4501 tagataaatc atcgctgtct ttcagcatca gaacatcgcg aataaacatc ttcatgccga
     4561 tcggaatatt acacacgccg ataaagcgtt taaatccggt atgacgataa acggcttcag
     4621 tgaccattcc cgccgggtta gtgaagttaa tcacccatgc attcggacaa agttcttcga
     4681 catcttttac gatgtcaaaa atcaccggaa tggtacgcag acctttaaac agaccgcccg
     4741 cgccgttggt ttcctgacca agataaccat gacttaatgg aatacgttca tccagttcgc
     4801 gcgccggtaa ttggcctacg cgcagttggg tagtaacgaa atcagcatct ttcaatgctt
     4861 cgcggcgatc cagcgtttta taaagcttca tcgggacgcc agcgttatca atcatccgtt
     4921 ggcagagttc aaaaatgata tccagtttct ctttaccatc ttcgacatcc accagccata
     4981 attcgctgac cggcaattcg tgataacgct taataaatcc ttccagtaac tccggggtat
     5041 agctgctccc gccaccaata gtgacgactt ttaatttctg gctcataatt tctcccttca
     5101 gtacagaata ctgatatctg gcatatctgc ccccccggac ataaataatc cagcaacagg
     5161 acagatatgt gaattgtcag gtataacgac ttactgatta aattcagtca atttcttacg
     5221 atagctctta ggcgtaaagg acgttaattt cttaaacgtt ttaataaaca aactcgggct
     5281 actataaccg gcctcaaacg caatatccgt cacggaatag ttggtcattt ccagttgttt
     5341 tttggcaaaa ttaatacgga tctcattaat aatctgcatt ggtgttttgc cataatatcg
     5401 ttgagtcgct cgcgtcaaat attcctgtga tttggctgac aacgtcacca tattctccag
     5461 cgccgattca ctaaactgct ctttatcatg catcttttct accgtacttt tcagccactg
     5521 cggtacatca tcaatcacct gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa
     5581 agtaaccatc tcaacaaact cttccagccc tgtttcgcgg aaattcaatg aagaaatcac
     5641 tgtttccaca taggtgagaa aagcgttatt ggtccggtat acctgcgaag cgacaaagca
     5701 ataaggcaac aacggcaggt aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa
     5761 tatacgcgtg gcaccaaact cataaaaact ttgatggtgc gaacctaacg gaataaaaac
     5821 aaaatcgccc cgttccagta acacgcgctt accgttaatc tcctggaaat aacgcccggt
     5881 taataccaga gtaaattcat aatagtcgtg ctgatgcagc ccgctgatac tctcagtttt
     5941 gttatagata aacacatgga agtttttgcc attaaacaac tgctgttctc gggcagtggc
     6001 aatttccggc gcgttaatca ctggctgcat catcgactcc ttatgccttc agtttttcat
     6061 gaagctcaat taattcagta atcagttcac gcgcaagcat ggacgtcatt aaatgatcct
     6121 gagcgtggac cagcaccaga ctcactttca tcttaccttc gcccgcatcg ccttcaatca
     6181 gtttcgtctg taccagatgc gcttcattca atgccatccg tgactgatcc atcatggctt
     6241 ttgctgcagc aaaatcgccc tgcttcgcct gtttcagtgc cgcatacgcc aggctgcgcg
     6301 cttgtccgga gttgatgata agccccatca ccacttcttc cagctcttca gcttccgttt
     6361 gcgtatcggg aatgttatcg agatccatca tacatcgttc ctctctattc ttaccggcac
     6421 gattacccgt accggcatcg attaaaattt cagggcatta gcgatatctt cttcgctctc
     6481 ttctttatca atcgcgtttt gtgctttgtt agccaccaca acaaagggca gataaattaa
     6541 cgttgcgata ccaaggttga agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt
     6601 aaagaaggct cccagaccgg ttggcatggt ccacggtgca atattggtca ccggaggaat
     6661 gatgcccatg tagtacgctg cgagggtgat tgccgccaga atcggttgta ccagtacaaa
     6721 cgggataaac atcaccgggt tcatgataat tggcagacca aacagaatcg gttcgttaat
     6781 ctggaagatg ccggacggca gcgccagttt tgccacctga cgataatcag cacggcgaga
     6841 ggcgataaag atagccagga tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa
     6901 ggagtccagc atcggcttgg cccagatatg gaaggtctta ccggctgcca gcgccgcttc
     6961 gacggaacca tattgctgat aggtcgcgat attttccagc gcccacggcg tcataatgcc
     7021 gttgtccagt gcggtcagcg ccagcgcgcc atgaataccg aagaaccaga gcagtggaac
     7081 aaagatcaca taggcccagc ccaccacgct acccaacgat gccagtgggg ttgagatggt
     7141 atccataatg atctgatgga agttggtgcc ccaggtattc aacgcccagg caataatccc
     7201 catcacggaa agaataataa agccgggaat taatgccgag aaggaacgcg atactgaagc
     7261 aggtacgctg tcgggcagtt taatgaccca attgcggcgg acaataaagg taaacatttc
     7321 tgccaccacc aggccaataa taatcccgga gatgatattc gccccaccta accagtttgc
     7381 gccaaccgca taggcctcac cgacactata tggggtgacg gtcataaatg ctgcaacgga
     7441 taacaaccca gccgccagcg catcgacttt acgctcttct gccagcgcca tgccaataaa
     7501 gaacggtgcc attaaagaca ttatccctaa tgttccgtta tatacgttgc cgccaatacc
     7561 tttcagacca ttaagtgttt caatggttga ggcgtcgagg cgaataccta aggaataaaa
     7621 aaacgacccc tccccaaagc ttagaaaaac gttgttaatt aatacaaaca tggcccccgc
     7681 aagggttaac ggcattaagc gaataaagcc atttttgatt gcattaacgt gtggctgctt
     7741 tcctatttta actgcaaaag ggaggagtac cttttcaagc gatgcaataa cattactcat
     7801 agaaaaatac ccttaa
//
LOCUS       DAZFWP020000203         7757 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFWP020000203 DAZFWP020000000
VERSION     DAZFWP020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.204"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHK0Z_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHK0Z_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU4504405.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHK0Z_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHK0Z_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU4504406.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAANGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHK0Z_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHK0Z_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU4504407.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4937..5551
                     /locus_tag="ACHK0Z_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             4937..5551
                     /locus_tag="ACHK0Z_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU4504408.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            5590..7041
                     /gene="trkH"
                     /locus_tag="ACHK0Z_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             5590..7041
                     /gene="trkH"
                     /locus_tag="ACHK0Z_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU4504409.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            7053..7598
                     /gene="hemG"
                     /locus_tag="ACHK0Z_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             7053..7598
                     /gene="hemG"
                     /locus_tag="ACHK0Z_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU4504410.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDHCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         1725 a         2097 c         2110 g         1825 t
ORIGIN      
        1 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
       61 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
      121 tggacgtctt tgcgctccat cagatttagc agcgtggtgc tgatacgcgc accggaacag
      181 cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc
      241 tccattagcc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcattcatt
      301 tcaaacacgc cgatatcact gacagaaagc cccgcttttt tcagcgccag cttcgaggcc
      361 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcacg
      421 cgggcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
      481 gctgcgccat cggaaagcgc agaagatgta cccgccgtta ccgtaccgct tactggatca
      541 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
      601 taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat aatttcattt
      661 ttaaatgcac ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
      721 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
      781 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtggctc
      841 attggcacat ggcccatatg ctccacgccg ccgacaagac atgcctgcgc atcgccagtc
      901 atgatcattc gtgctgcgtc atgcaatgcc tgcatggatg aaccacacaa gcgattgacg
      961 gtcaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatatta
     1021 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
     1081 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
     1141 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
     1201 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
     1261 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
     1321 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
     1381 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
     1441 accagcgcca tatccgcttc cgccggagtg gcgataatgc cttcctccag acagcgcacc
     1501 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
     1561 ttcggctggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
     1621 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
     1681 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
     1741 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacgac atccagcaga
     1801 tatgccggac ccataggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
     1861 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
     1921 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
     1981 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
     2041 ggcatccggt gtaccgggtt aaaaaagtgc attccgcaga agttttccgg gcgttccagc
     2101 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatccggg
     2161 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcgacaacc
     2221 gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg gtggattgtg
     2281 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
     2341 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
     2401 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
     2461 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
     2521 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
     2581 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
     2641 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
     2701 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
     2761 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
     2821 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
     2881 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
     2941 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
     3001 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
     3061 tcgcagccac cgcccagcgc atagccgttg gcggcagcaa tggtcggcac cggcagatct
     3121 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
     3181 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
     3241 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
     3301 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
     3361 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
     3421 gtctcctgaa tccgcaaggt gatctggtcg taccagatga gtcgaagtgt gtattttgtg
     3481 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
     3541 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
     3601 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
     3661 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
     3721 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
     3781 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
     3841 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
     3901 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
     3961 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
     4021 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
     4081 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
     4141 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
     4201 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
     4261 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
     4321 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
     4381 cccgtacctg gtcggcaaaa agtgacaacg actacgcaca gctggtgaaa gacgtaaatg
     4441 atgaacaact ggcgctgatc gctaccatga aagcaggcgt cagctatgtg gattaccaca
     4501 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
     4561 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
     4621 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacgc
     4681 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
     4741 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctgctggca ccgtggcgtg
     4801 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
     4861 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
     4921 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
     4981 agagatcaaa aaaagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
     5041 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
     5101 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
     5161 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
     5221 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
     5281 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
     5341 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
     5401 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
     5461 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
     5521 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
     5581 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
     5641 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
     5701 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
     5761 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
     5821 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
     5881 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
     5941 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
     6001 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
     6061 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
     6121 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
     6181 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
     6241 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagcccgact
     6301 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
     6361 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
     6421 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
     6481 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
     6541 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
     6601 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
     6661 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
     6721 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
     6781 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
     6841 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
     6901 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
     6961 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
     7021 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
     7081 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
     7141 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
     7201 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
     7261 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
     7321 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
     7381 tgaactcgca atggcgtccc gatcactgcg cggtcattgc cggggcgctg cgttacccac
     7441 gttatcgctg gtacgatcgt tttatgatca agctaattat gaagatgtca ggcggtgaaa
     7501 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
     7561 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
     7621 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
     7681 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatc
     7741 tcaacagaga aaagtaa
//
LOCUS       DAZFWP020000204         7597 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFWP020000204 DAZFWP020000000
VERSION     DAZFWP020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.205"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            5..607
                     /gene="yebB"
                     /locus_tag="ACHK0Z_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /pseudo
     CDS             5..607
                     /gene="yebB"
                     /locus_tag="ACHK0Z_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
     gene            complement(609..1130)
                     /gene="ruvC"
                     /locus_tag="ACHK0Z_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             complement(609..1130)
                     /gene="ruvC"
                     /locus_tag="ACHK0Z_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU4504411.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1165..1905)
                     /gene="yebC"
                     /locus_tag="ACHK0Z_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             complement(1165..1905)
                     /gene="yebC"
                     /locus_tag="ACHK0Z_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4504412.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1934..2386)
                     /gene="nudB"
                     /locus_tag="ACHK0Z_RS22600"
                     /old_locus_tag="pgaptmp_004520"
     CDS             complement(1934..2386)
                     /gene="nudB"
                     /locus_tag="ACHK0Z_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU4504413.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2504..4276)
                     /gene="aspS"
                     /locus_tag="ACHK0Z_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     CDS             complement(2504..4276)
                     /gene="aspS"
                     /locus_tag="ACHK0Z_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU4504414.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4586..5152
                     /gene="yecD"
                     /locus_tag="ACHK0Z_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             4586..5152
                     /gene="yecD"
                     /locus_tag="ACHK0Z_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4504415.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5149..5244
                     /locus_tag="ACHK0Z_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /pseudo
     CDS             5149..5244
                     /locus_tag="ACHK0Z_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5470..5718
                     /locus_tag="ACHK0Z_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             5470..5718
                     /locus_tag="ACHK0Z_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4504416.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(5715..5801)
                     /locus_tag="ACHK0Z_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /pseudo
     CDS             complement(5715..5801)
                     /locus_tag="ACHK0Z_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            5886..5969
                     /locus_tag="ACHK0Z_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /pseudo
     CDS             5886..5969
                     /locus_tag="ACHK0Z_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(6090..7103)
                     /gene="nleC"
                     /locus_tag="ACHK0Z_RS22635"
                     /old_locus_tag="pgaptmp_004527"
     CDS             complement(6090..7103)
                     /gene="nleC"
                     /locus_tag="ACHK0Z_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HGU4504417.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVISAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(7507..>7597)
                     /locus_tag="ACHK0Z_RS22640"
                     /old_locus_tag="pgaptmp_004528"
     CDS             complement(7507..>7597)
                     /locus_tag="ACHK0Z_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4504418.1"
                     /translation="ADKKQIQCVVRPLQILRADGTWENVGGMK"
BASE COUNT         1876 a         1991 c         1763 g         1967 t
ORIGIN      
        1 caacgtgaat attaattacc ctgctgaata tgaaattgga gatatcgtct ttacatgtat
       61 aggtgctgcc ttatttggtc aaatatcagc tgcatcaaat tgctggagta atcacgtcgg
      121 gatcattatc ggtcataacg gtgaagactt cctggttgca gaaagccggg ttcccctttc
      181 aaccatcacc acgctatctc gttttattaa acgttcgtct aatcaacgct atgctataaa
      241 gcgattagac gctgggctga cagaacaaca aaaacaacga attgttgaac aggttccctc
      301 ccggctacgc aaactttacc acaccggttt taaatacgaa tcttcgcgcc agttctgttc
      361 aaaatttgtt tttgatattt ataaagaagc gctatgcatt ccggtgggtg aaatagaaac
      421 gtttggagaa ctgttaaata gcaatccgaa tgcaaaactc actttctgaa aattctggtt
      481 cttaggttct attccgtggg agcgtaaaac cgtcacgcca gccagtttgt ggcatcatcc
      541 gggtttggtg ttgattcacg cggagggagt tgaaacgcct cagcctgaac tgaccgaggc
      601 ggtataactt aacgcagtcg ccctctcgcc aggttcagcc gcgattcgct catctgcatc
      661 gcattctgac taacgtggca gtgggtgatg gcaatcgcca gtgcatcggc ggcatccgct
      721 tgtggattag cgggcagttt cagcaaggtg cggaccatat gctgcacctg gcttttctcg
      781 gcgctaccaa tgcctaccac cgtttgcttt acctgacgtg ccgcgtattc aaatactggc
      841 aactcctgat tcaccgccgc cacaatcgcc acgccgcgcg cctggcccag tttcagggct
      901 gagtcagcgt tcttcgccat aaagacttgt tcaatggcga aataatcagg ctggaactgg
      961 gtgatgattt ccgtcacgcc cgcatagatg agcttcagac gagacggtaa atcatccact
     1021 ttggtgcgta tgcatccgct acccaggtag gacagttgcc tgcctacctg gcgaattacg
     1081 ccatagccgg tcacgcgcga acccggatca atgccgagaa taatagccat cacgcgtctc
     1141 cgttttgctg tttagcaggc ctcatcagag agtcgctgcg acctcatcag agatttcacc
     1201 gttatggtaa acttcctgca cgtcgtcgca atcttccagc atatcgatca gacgcatcag
     1261 tttcggtgcg gtttctgcat ccatatcagc tttggtggac gggatcatgg aaacttccgc
     1321 gctgtctgct ttcagacctg ccgcttccag agcgtcgcgc actttaccca tttcttccca
     1381 tgcagtgtag acatcaatcg cgccgtcatc ataggtcaca acgtcttcag cacctgcttc
     1441 cagagctgct tccatgatgg tgtcttcatc gcctttctcg aaggagatca cgcctttttt
     1501 gctgaacaga taggctacgg aaccatctgt accgaggtta ccgccacatt tgctgaatgc
     1561 atgacgcact tcagcaacgg tacggttgcg gttgtcagac agacattcaa tcatgattgc
     1621 cgtgccgcca ggaccgtaac cttcgtagat gatggtttcc atgtttgcat catcatcacc
     1681 gcccacacca cgtgcaattg cgcggttcag tgtgtcacgg gtcatgttgt tagacagtgc
     1741 tttatccacc gccgcacgca gacgcgggtt agcgtccgga tcgccaccgc ccagcttagc
     1801 cgcggttacc agctcacgaa tgattttagt gaagatttta ccgcgcttag catcctgcgc
     1861 agctttacga tgtctggtgt tggcccattt actatgacct gccataaaaa tatctccaga
     1921 tagccctgcc tgttcaggca gcgttaatta caaactgttc aatcgcctgc cggttgctcc
     1981 aggacttagt gagcgccgcc gcagcaggcg catcaagcca cttgtaagcc agatgttcag
     2041 tgaaaacgat ctgccgctcg tgcggaagcg caagacagaa ccatgattcc gtattacgcg
     2101 tcacgcccgg cgcatagcga tgacgtaaat gtgaaaaaat ttcaaactct accgtgcgct
     2161 gacagtcaat taaggtcagt tgttcagcga caacatcaat ggtgacctct tcctttactt
     2221 cgcgcatggc agcttgcggc gcggtttcac cctcttccac gctgccggtt accgactgcc
     2281 agaaatcggg atcgtcacgc cgctgcaaca tcagcacccg tttcgtatct tgtgcataga
     2341 tgaccactaa gatcgaaacg ggacgcttat acactttatc ctccacgctg cctcttcgtt
     2401 gactgccttc gctcatccca ttcacatagt tatctatgct catgggagtt cactcagttg
     2461 ccgccttgat gcaacgcgaa tgatttcgtg tatttgagtc atatcagtta ttctcagcct
     2521 tcttcacaac ctgaatgctc agctcagcca gtgcagtcgg gttagcaaag ctcggtgctt
     2581 cagtcatcag acacgctgcc gccgtggttt tcgggaaggc gataacgtca cggatattgt
     2641 cggtgccggt cagcagcatg gtcagacggt caagaccgaa tgccagacct gcgtgcggcg
     2701 gagtaccgta tttcagagcg tcgagcagga agccgaattt ctcgcgctgt tcctcttcgt
     2761 tgatacccag aataccaaac accgtctgct gcatatcacc attatggata cgtactgaac
     2821 caccgcccac ttcgtaacca ttgatgacca tatcgtaagc gttcgccacc gcattttccg
     2881 gtgcagcttt cagttctgca gcagtcatgt ctttcggtga ggtgaacgga tggtgcattg
     2941 ccgtcaggcc gccttcaccg tcgtcttcaa acatcgggaa gtcgataacc cacagcggtg
     3001 cccatttgct ttcgtcggtc agaccaaggt ctttacccac tttcaggcgc agtgcgccca
     3061 tcgcgtcggc aacaattttc ttgttgtcgg caccgaagaa aatcatatcg ccatcttgcg
     3121 cggcagtacg atccaggatg gcttcgatga tttctgcatt aaggaacttc gctaccgggc
     3181 tgttgatacc ttccagacct ttcgcgcgtt cgttaacttt gatgtaagcc agacctttcg
     3241 cgccgtagat tttaacgaag ttaccgtatt cgtcgatctg cttacgggtc agcgatgcgc
     3301 cgcccggaac gcgcagagcg gcaacgcgac ctttcggatc gttcgccgga cctgcaaata
     3361 ctgcaaactc aacagatttc agcagatcag caacgtcggt cagttccatc gggttacgca
     3421 gatccggttt atcagaacca taacggcgtt ctgcttccgc aaaggtcatt accgggaaat
     3481 cgcccagatc cacgcccttc acttccagcc acagatggcg caccagcgct tccatcactt
     3541 cacgcacttg cggcgcggtc atgaaagaag tttccacatc gatctgagta aattcaggct
     3601 gacgatcagc gcgcaggtct tcgtcacgga agcatttaac gatctgatag tagcggtcaa
     3661 agccggacat catcagcagc tgtttgaaca actgcgggga ttgtggcagc gcgtagaatt
     3721 tacctttgtg cacacgagaa ggcaccaggt agtcacgcgc gccttccggc gtggctttgg
     3781 tcagcatcgg agtttcgatg tcgaggaagc cgtggtcatc cataaaacgg cgcaccaggc
     3841 tggtgatttt agcgcgggtt ttcaggcgct gagccatttc cgggcgacgc aggtcgaggt
     3901 agcggtattt cagacgcgct tcttcggtgt tgacgtggtt agagtcaagc ggcagaacat
     3961 ctgcgcggtt gatgatagtc agcgaggacg ccagcacttc gatttcgccg gtcgccatat
     4021 cgcggttaat atttttttcg tcacgcgcac gtacggtgcc cgtgacctga atgcagaact
     4081 cattacgcag ttcagaggcc agctttaacg cgtccgcacg atccggatcg aaaaatacct
     4141 gcacgatacc ttcgcggtcg cgcatatcga tgaagatcag gctaccaaga tcacgacgac
     4201 ggttgaccca accacacaga gtcacctgct gccccacgtg ggacaaacgg agctgtccac
     4261 aatattctgt acgcatgaga tatcccttaa cttagctgcc ggcggatgcc ccctgctgcg
     4321 caggtgacca agtcgcagcg ttagctgtat gtcacaactg aatgaaaaaa ggcggctatt
     4381 atactggaaa ttctgccgca ccgtaagagc ctggcccgcg ctggaacgcc tcgttaccac
     4441 tttatatcgg gcctgaaatc agactctacg ccagtttgct ataaaggtgt tgcccgaact
     4501 cataaaaatt aacaaaattt gtcgttccgc catcggctaa tcgcattaag gtgtgaggca
     4561 cgattttgtt ttttcaggag tcattatgct tgaacttaat gctaaaacca ctgcgctggt
     4621 ggtgatcgat ttacaagaag gcatcttacc ttttgccggt ggtccacata ctgccgatga
     4681 ggtagttaat cgcgccggga agctggcggc gaaatttcgc gccagcggtc agcccgtgtt
     4741 tctggtgcgc gttggctggt ctgccgatta cgccgaagca ttaaaacagc cggttgatgc
     4801 cccctccccc gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc
     4861 aactgacagt gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct
     4921 ggagttgcaa ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa
     4981 tatcggtgtt gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc
     5041 cgaagacgcc tgtagtgccg ctagcgccga gcagcacaat aacagcatca accatatcta
     5101 cccacgcatc gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc
     5161 ggtctaccgc aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag
     5221 tatgcccgcc actttaactg catgacgcaa catttttaaa aatcgacgaa gatcgtccag
     5281 gagcgccgtt tttcaagggt tggatagatg attcgtttac catgtcgacg aacttcgacg
     5341 aatttcgccg ataaaaagtg tcccatacat gccccattga tgccccataa acaatatcag
     5401 gaattgacac tggttatcca tacagataaa aataacacct gtatataacc acagttacag
     5461 gagggtgtta tgcgtgttga gatctgtatt gctaaagaaa aaatcagcaa aatgccaaac
     5521 ggtgctgtgg atgcgttaaa ggaagaatta acccgacgca tcagtaaacg ttatgacgat
     5581 gtagaggtga tcgtaaaagc caccagcaac gatgggcttt ctgttacacg caccgcagat
     5641 aaagattctg caaaaacatt tgttcaggag actctgaaag atacctggga gtctgctgac
     5701 gagtggtttg ttcactaata aacacgtaaa atcggtaacg gctggaaatc attcaatact
     5761 cgcactatcg aaagttcacc agccaaccgc gacacgctct tacatacgaa gtgccgcgct
     5821 ttccttaata attttttagc agtactgtgt aaatgatgag cgacctaatc catgcatgac
     5881 gcgtagtgcc tattgtgcat ctttctgcgt ctctttttac tggacccaag ccaatgatgc
     5941 tggcgatacc attcccgcca ggcccactta caacagtaaa attcggagct ctgcctttac
     6001 atacaataca atgttgcacg attgcccatc ataaactccg ttaatatcta tgagtagtaa
     6061 ccctatgtaa ctgtaaagac gaatcagcgc taattgtgtc tgtccacatc cccaaatgta
     6121 aagatataag gagcaccatg atacagttgg atctgattta catcctgtaa attctcgtcg
     6181 atagccaccc ctggaaaatc atgttgctgg ataaatccgt atcctatgtt agacacagcg
     6241 gaatactctg tgaaatcagg actcgcctca tatcggtcac tgatcgtacc cagtctttcg
     6301 aagaaagccc tctcattctt ttcatgcctc atggcagcct gtcgaagggc attcaggtta
     6361 cgtagtctaa gatgagcttc tcgttctggc tctgcatatc ctttgaagtc gggaacactc
     6421 catcccagct cttgagcgac acgacgtgcg agaatctcgg tgggtcctag ctctatatta
     6481 ctatctccag atggatcgct ggccccagta acatgatgaa taatctcgtg aatcagtcct
     6541 tcctgccacg atggcatctc ataagaatca gtatctggcg caacactaaa actgacataa
     6601 ggctcttcgt tctcgttttc tcctgcctcg caaatgggga gaattggttc ttgcccgaca
     6661 tcgtatccat ataattcgtt acaggttagt gattgaatat catcaatttt gatagcagat
     6721 tctccgttaa gctcgtattc gtttctgtat ttaatgcaac caatgtgtac ctcctcatta
     6781 tgaatgccat agcttacggc atcacgaaat gtttgtgatc ggctcaacgc atcaagtaca
     6841 gtgttgccaa tcatatcgac cgtatgctga tcgatgagcc tgctacggct gtcatggacg
     6901 gcgctaatta cactttcgta tacgttgctt aaatctgctg cggaaagcgg tattcgctta
     6961 cctatatcca aaacgtaatc cgcataggca ttttcagcac gattaggagc aacggcagca
     7021 gagtatccag caggggcaaa aaagttgaag ccaggttgga gaacgggaat tttcatattg
     7081 atacttaata tggttttata tatttcagtg ccaccaattc actcgcttac aaccagaaac
     7141 taaaaaaacg tcagggcgtt gataataatg aataccaact ttctgcccat ttatagtaat
     7201 atctttcgtt atcaggtttt gtctggcagc ttcacgtaat atttcaatac tctgcaaagt
     7261 aacatttgat gaaatgctgg atgttatcaa tggcatatgt catttacccc aacaagacta
     7321 aattttagaa aaaacaactg ccgggacaac tttcgccccc tgtaacccca aataagatgt
     7381 cagatttatc ggcattaatt tatctccctg ttgaattagc acctctatca tctacttaac
     7441 aaatatccca cggacattgc aacacaaaaa ccggagccgg actccggttt ttgtgaagct
     7501 gtcgggctat ttcatcccgc caacattttc ccacgtcccg tcagcacgca ggatttgcag
     7561 cggtcttacc acgcactgta tctgcttttt atccgca
//
LOCUS       DAZFWP020000205         7480 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFWP020000205 DAZFWP020000000
VERSION     DAZFWP020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.206"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..828)
                     /gene="rfbA"
                     /locus_tag="ACHK0Z_RS22645"
                     /old_locus_tag="pgaptmp_004529"
     CDS             complement(<1..828)
                     /gene="rfbA"
                     /locus_tag="ACHK0Z_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4504419.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFMRLLGDGSQFGINLTYAIQPSPDGLAQAFIIGENFIGNDTV
                     CLMLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYFYDNNVIDIAKCIKPSKRGELEITAVNEVYLRNNNLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLNHDELQAKGEFLKKN
                     "
     gene            complement(825..1901)
                     /gene="rfbB"
                     /locus_tag="ACHK0Z_RS22650"
                     /old_locus_tag="pgaptmp_004530"
     CDS             complement(825..1901)
                     /gene="rfbB"
                     /locus_tag="ACHK0Z_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4504420.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSKRYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            complement(2273..3166)
                     /gene="galF"
                     /locus_tag="ACHK0Z_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             complement(2273..3166)
                     /gene="galF"
                     /locus_tag="ACHK0Z_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU4504421.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(3341..4735)
                     /gene="wcaM"
                     /locus_tag="ACHK0Z_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             complement(3341..4735)
                     /gene="wcaM"
                     /locus_tag="ACHK0Z_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU4504422.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARPSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINIGIFIPAGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMTGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            complement(4746..5966)
                     /gene="wcaL"
                     /locus_tag="ACHK0Z_RS22665"
                     /old_locus_tag="pgaptmp_004533"
     CDS             complement(4746..5966)
                     /gene="wcaL"
                     /locus_tag="ACHK0Z_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU4504423.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGLVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5963..7243)
                     /gene="wcaK"
                     /locus_tag="ACHK0Z_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     CDS             complement(5963..7243)
                     /gene="wcaK"
                     /locus_tag="ACHK0Z_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU4504424.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCNALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARHNEAVSRERHTGMQMVQSVLERIGEVK"
BASE COUNT         1863 a         1872 c         1719 g         2026 t
ORIGIN      
        1 attttttttc aaaaattcac cttttgcctg taattcatca tggtttaacc agccatttcg
       61 ccatgcaatc tcctccggac aagcaaccat catcccttgc cttttttgaa ctgtctctac
      121 aaaactccca gcttcaatca agctgtcatg ggttcctgta tccagccatg caaaaccacg
      181 tcctagtaat tcaacagtta aattattatt tctgagataa acttcattaa ctgccgtaat
      241 ttctaattca ccacgtttag aaggtttaat acactttgca atatcgataa cgttattgtc
      301 ataaaaatat aatcctgtga cagcccaatt tgattttgga ttggcaggtt tttcttcaat
      361 acttaaagct ttaaaattcc tatcaaactc cactactcca aatctctcag gatccattac
      421 ttgatatcca aagactgtgg cacctgatgt tctttctgct gcatcccgga gtttgggtgt
      481 aaaaccttgt ccaaaaaaaa tgttatctcc aagcataagg catactgtgt catttccaat
      541 aaagttttca ccaataataa atgcttgggc taaaccatct ggactaggtt gaattgcata
      601 tgtcagatta ataccaaatt ggcttccatc cccaagtaat ctcatgaacc cttgctgatc
      661 ttctggcgtt gtaataataa gaatatcttg tatacctgcc aacatcagca ccgacaaagg
      721 ataataaatc atcggtttat cataaatggg taatagctgc tttgaaactc ccattgtaat
      781 tggataaagc cttgttccag aaccaccagc taaaataatt cctttcatct aattaccccc
      841 aaacgctcac gttgataaga gccatcttga actcgccggc accaatcatt attattcaaa
      901 taccagttaa ttgttttacg aatcccgctc tcaaacgttt cctgtggttt ccatcccaat
      961 tcgcgaccaa tcttctcagc atcaatggca taacggcgat cgtgtcccgg acgatcggca
     1021 acataagtaa tttgctcgcg gtaagatttc tctttcggta caatctcatc caacaaatca
     1081 caaatagtga gcactacatc gatgtttttc ttttcgttgt gtccaccaat gttataagtt
     1141 tcacccgctt taccttcggt tacgacggta tataacgcac gtgcatgatc ttcaacatac
     1201 agccagtcgc gaatttgatc ccctttacca taaataggta atgccttacc ttccagagca
     1261 ttaagaatta ctagtggaat caatttttcc ggaaagtgat aaggaccgta gttattcgaa
     1321 cagttagtca caatggtcgg taaaccatag gtacgtttcc acgcacggac taaatgatcg
     1381 ctagatgctt ttgatgcgga ataagggctg cttggcgcgt aagctgttgt ctcagtaaat
     1441 aagggtaatt cttctgtatt atttacctcg tcaggatgag gcaaatcacc atatacttcg
     1501 tcagtagaaa tatgatgaaa acggaagcta tttttcttgt cgctatcaag agcagaccag
     1561 taattgcgag cggcttccaa aaggacataa gtaccaacaa tattggtttc aataaatgcc
     1621 gcagggcctg taattgaacg atccacatgg ctttcagcag ccaggtgcat cactgcatcc
     1681 ggctgatgct gagcaaaaat ccgcgccatt gcagcagcat cgcaaatatc cgcatgttca
     1741 aaaacatagc gtttagagtc agaaacatct gcaagtgatt ccaggtttcc ggcgtacgtt
     1801 aatttatcga cattaacaac actatcctgc gtattattta taatgtgacg aactacagca
     1861 gaaccaataa atcctgcgcc accagtaaca agtatcttca ctttattatt ccatattact
     1921 tcagagcatg ctgtgaaata agcggctctc agtttgatta atagaggtat taatgcacgc
     1981 taccgcccct ggctttacag ctaccagagc actgcataca tgtccacgat gtgacgagcg
     2041 ttacccactc gcgctaaacc cgaaaaattc aaacgctaat tgtcttacca atccgctctg
     2101 gaaacaagga aaatcctgga aaactttgac taaaatccta ttgctaactc gttgttattc
     2161 tgattgttta tataaaacaa cggcaggaaa attcgcaaca aattactttc gccacgaatc
     2221 ttcactgccg taataatttt cttatcaacc gttacattcg gtcagatttt cattattcgc
     2281 ttaacagctt ctcaatccct ttacggaact tcgccccttc tttcaggttg cgcagcccat
     2341 acttcacgaa cgcctgcata tagcccattt ttttaccgca gtcgtagctg tcacctgtca
     2401 tcagcatggc atcaacggac tgttttttcg ccagttcagc gatggcatca gtcagctgaa
     2461 tacgccccca tgcaccaggc tgagtgcgtt caagttccgg ccaaatatcg gcagaaagca
     2521 catagcgacc aacggccatg atgtctgagt ccaacgtctg cggctgatcc ggcttttcga
     2581 taaattcaac aatgcggctg actttacctt cacggtccag tggttctttg gtctggatga
     2641 ccgagtattc agagaggtca cccggcatac gttttgccag cacctggcta cgacccgttt
     2701 cgttgaatcg tgcaatcatg gcagcaaggt tgtagcgcag cgggtcggcg ctggcgtcgt
     2761 cgatcacaac gtctggcagc accacgacaa atggattgtc accaatggca ggccgcgcac
     2821 acaaaatgga gtgacctaaa cctaaaggtt cgccctgacg cacgttcata atggtcacgc
     2881 ccggcggaca gatggactgc acttccgcca gcagctgacg cttcacgcgc agttcaagaa
     2941 gagattctaa ttcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag
     3001 ttaccaggag gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca
     3061 ttggcttgtc gacgatcggt agcatctctt tgggaatcgc cttagtggca ggcaacatat
     3121 gcatcccaag acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct
     3181 gtaaaatggt tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga
     3241 attgattact gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt
     3301 tcctaagtat ggctccattt ttccaggaat ggtcgcaaat ctactctctc cgttccggca
     3361 atctaaagtt aatcttttcc acattaacaa tatggtgatt aaccctatcg atatcgacgg
     3421 agctttgtcc tttctcattc accgcatgaa catttgcaag agatagcagg gtttcttttt
     3481 tcgccataaa gacgccgcga acgtctttgc gcatgtcaaa gttcatgctc aatgcgggtc
     3541 caacagagga ttcctgcatc actttgatat tacgcataaa aagatgttgc ggtttgttgt
     3601 gtaactccag agacgcacgc ttcatctcaa tgttagtcag tgccacaaag gagacggcat
     3661 tcccggcaga gatttggata ccgcgcaatt tgtaagcaag gtgggtatta tccagttgaa
     3721 tattattcac tcggaaattt tgtggtatcg agagatattt gcctttaatt accccatagc
     3781 cgattaacat tccggcacta ttaatcattt caatattatc aatcacgaaa ttatcacaac
     3841 cgtaaatagc gactgtcgcg ttatcaatac ctgctttctt actgaaatcc ggcgtgatat
     3901 tgcgggcttt gatattacgg ataacaaaat gtttaccatt ttcaacatgg atcaactgcc
     3961 gacaatccga tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt
     4021 aattattatc ataagtgctt cccgcaagac ctatgccaat gccccagttg attttgccgt
     4081 tagtacagtt gatgcgctcg atgatgtggt cggagatcaa aatatcactg tcgttaattg
     4141 ccacgttcca ttcaatggcg tcgccttgta agtcgctgaa cttacagttg gtgatgttgg
     4201 caccgataat ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag
     4261 tgaggttatc gatggtcaag ttacgcatga cccgtttgtt tttgccgccg atatagatct
     4321 gcgtcaccgg gccaaagccg gtcatcgtca gccctttgat ggtgcagtca gaaccacgca
     4381 catccagggt gatgttatgc atactaccgc cctcctcccc tgtcacctgg ctgccgtcct
     4441 gtaaaacaaa tcgccctttg ccgttgccgc gtaggcttcc aaggatgtgt aacgttttac
     4501 ctgcaggaat gaagatgccg atgttgatat tgtcacaaac caatccggca ggcacgacga
     4561 ccgtttgccc ttcactaaaa gcttgtttaa atgaggcgat ccagtcgtgc ggattgtaat
     4621 cgttgatatt aacgcttggt cgagcaggaa gtgcgcgagc gaaaggggta tggaggaagg
     4681 caagcgccga gcttgccgtc aggaaggtgc gtcgggagag ttttttaaat ggcatacgtt
     4741 ctcctctata aagcctgcag caagctggcg agttctcggt taatcacctg ctggttaaaa
     4801 tcgtgttcga ctttttcgcg cgcacgtttg acgaccggag ccagttcgtc ggtgtccagt
     4861 tggctaaacg ccgccagtcg ttgcgccagt gcgcgagcat cgttctcagg caccagccag
     4921 ccggatttat cggcctccac caattccggt attccgctat gcagagtaga aaccaccgga
     4981 atgccgaccg ccatcgcttc catcagcgct accggaatgc cttccatatc gccatccgcg
     5041 cccgttaccg atggcaacag gaagacatcc gcgtcgtcga gcatcgcttt cacttcgtgg
     5101 ctcggtttaa agcccggcat ctctaccaca tcttccagtt gatattgttc gatgagggta
     5161 cgcaggcgtc gttcccacgg gccaatgccg aggatgcgat agcgaaatgc catgccctgc
     5221 tctttcaact gacggcaggc ttcgatcgcc acatgcaggc cttttttttc ggttaagcgt
     5281 gcgacggaga tgatttccag cggtgttgcg ggcgctttca cgggacgcgg gctaaagcgc
     5341 gtcatatcta cacccatgcg cgatacggcg attttttccc tcgggcagcc cattttttgc
     5401 agccttccgg cccacagatc gcttatcggt agcatcaggt cgccacgtcg aaacagttgt
     5461 tgatattcgg gagtgtaatg gttgagcact tcccgactgg aaatatcaat gccgtggaaa
     5521 atagtggcaa ttttgccgcg aatgacaccc agttcgcgga gttttgctgc ggttaccccc
     5581 gccgggccaa aatgagcgat aaagacatcg gcatgaaacg gtgttgcgac caggccgcaa
     5641 atggcagaca aaatcaggtt tcgcgactca gcaccatagc gtttgaggtt gagcgcctgc
     5701 caggtatttt tacgatgaat gccgcgtaag gtctggctgg cgcggtggcg cagtttcgca
     5761 actttgcccg taggttcgtc ctgtaaccag cgggttctgg cggcaaggtt gtatttcgtc
     5821 catgccgcgt gggtgttttg tgtgtcgcct ttttgcagcg cgacaatctc cacctcaaag
     5881 cccatatcaa taaacgcggt aatttgattg aggacgaagg tttccgacga cagcggaaat
     5941 ttcaataaga agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca
     6001 ccatctgcat tcctgtatga cgctcacgac tgacagcttc gttatgtcgc gcgttcagcg
     6061 ccggaagctg gcctaaggta tccgcaacca tcgcttgcag gctgccgtct aataaatgac
     6121 ggatatcaat tgccatctcc ggtagtccca gctgctgcat aatcccggcg gatttatgtt
     6181 cgtagttgat ggcaattgcc ggagtggaga aattcatcga gataatggca gagtgcaggc
     6241 gcgtaccgac ggtgagttca caggcaccga gaattttgcc catttccaga tcgttgagtt
     6301 catccatcac tacgtgataa cgggcaggat cgctgatgtg ctggcgcagg ttgagcgcca
     6361 ccatgcggtc gtctttgtta tagctgtcga tgcccgtaca ggtggagagc gcaatcacct
     6421 gatacccttc gtcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgct
     6481 gagtggtgcc gaggcgtttg tcgaacggtg ccagttcgcg cagggtaatt gccaccgttt
     6541 tctgttgtgc ggcaacgtcc agccagtgtt ggacggcata gctggcggtg aaatcttcgg
     6601 tgtggtgatc gaccagccac gcggtatcga cgccatgttc cacttttgca gtggtgatat
     6661 tgctgcgttt catcagttcg agactgaccg attcgcgcag gatcagcgcg ttacagtgac
     6721 caaaaacgta gttcgccagt tggttaaatt gctcatcctg gaagggaccg acgctgtgac
     6781 caatcataaa cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca
     6841 cgccgtagag atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaga
     6901 gcaggcgcac gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt
     6961 cggtgacgcg tgagagcaat acctggtgct gatagcggcg acggaggact tttttaacgc
     7021 gcccgacaac gcccgccgcg ctgttgtgtt gtttcatttg caggaacagc ggatcgccca
     7081 ttaccgggcg gttgagcagc caggaagaac tgaccggata gcggctcatc acatccactt
     7141 cggcgtgtgg attgaggatg ttgatggcat caagtaagcc gcgcaggatg gcgctgtcgc
     7201 cacgattgcc gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt
     7261 gatgccaggt gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg
     7321 gcgcgtctta tcaggcctac aagacccgag cacagaaccg taggccggat aaggcgtttt
     7381 acgccgcatc cggcattcag tgcctgatgc gacgctggcg cgtcttatca ggcctacaag
     7441 tcccgagcac agaaccgtag gatggataag gcgttttacg
//
LOCUS       DAZFWP020000206         7469 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFWP020000206 DAZFWP020000000
VERSION     DAZFWP020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.207"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1052
                     /locus_tag="ACHK0Z_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     CDS             <1..1052
                     /locus_tag="ACHK0Z_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU4504425.1"
                     /translation="KKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGNDL
                     EIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAF
                     AIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVAL
                     TFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDA
                     DKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRAL
                     AITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASV
                     IRTTRKIFSGEVYLP"
     gene            1128..2561
                     /locus_tag="ACHK0Z_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     CDS             1128..2561
                     /locus_tag="ACHK0Z_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU4504426.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2744..5005
                     /locus_tag="ACHK0Z_RS22685"
                     /old_locus_tag="pgaptmp_004537"
     CDS             2744..5005
                     /locus_tag="ACHK0Z_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU4504427.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKLGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5147..6430)
                     /locus_tag="ACHK0Z_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             complement(5147..6430)
                     /locus_tag="ACHK0Z_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU4504428.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6565..>7469)
                     /locus_tag="ACHK0Z_RS22695"
                     /old_locus_tag="pgaptmp_004539"
     CDS             complement(6565..>7469)
                     /locus_tag="ACHK0Z_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4504429.1"
                     /translation="SGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINY
                     MSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEG
                     HITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDL
                     CEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIAS
                     TRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLG
                     HKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1787 a         1778 c         1915 g         1989 t
ORIGIN      
        1 tgaaaaaaat accctgcgtg atgatgcgag gtggaacctc gaggggcgcg ttcttgttag
       61 cggaacattt acccgaagat caaacgcagc gcgataaaat attgatggca attatgggtt
      121 ccggtaacga tctggaaatt gacggtattg gcggcggtaa tccgctgacc agtaaagtcg
      181 ccattattag ccgttccagc gatccgcgtg ctgatgtcga ttatctgttt gctcaagtta
      241 ttgtccatga gcaacgtgtc gataccacgc ctaattgtgg caatatgctg tctggtgttg
      301 gggcattcgc cattgaaaat ggtttgattg cagcgacttc accagttacc cgcgtacgta
      361 tccgcaacgt caatactggt acgttcatcg aagctgatgt gcaaacgcca aatggtgttg
      421 tcgagtacga gggtagcgcc agaattgacg gcgtaccggg tactgccgca ccggttgcgc
      481 tcactttcct gaatgccgcc ggaaccaaaa ccggaaaagt cttcccgact gataatcaga
      541 ttgattattt tgacgatgtc ccggtgacct gtatcgatat ggcgatgcca gtcgtcatta
      601 ttccggctga atatctggga aaaacaggtt atgaattacc ggcggaactg gatgccgaca
      661 aagcattatt agcacgcatt gaatctatcc gtctacaagc gggtaaagca atgggcttag
      721 gtgatgtcag taatatggtt atccctaaac ctgtgcttat ttctccagcg cagaaaggcg
      781 gggcaattaa tgtacgttat tttatgccgc attcttgcca tcgcgcgctg gcaataaccg
      841 gtgctattgc tatttccagt agttgtgcat tggaaggcac cgtcacccga caaatcgtcc
      901 cttctgtagg atacggcaat atcaatattg aacaccccag tggtgcgctc gacgttcatt
      961 taagtaatga aggtcaggat gccacgacgt tacgcgcatc tgttattcgg acgaccagaa
     1021 aaatattttc cggtgaagtt tatcttccct gaaaaaattc gttgtcagga taaggacaat
     1081 caataaagga cttctgtatg agtcatacag aaagaacagg attttaaatg aataagaaat
     1141 cgttatggaa gctaattctg atattagcga tcccatgtat tattggtttt atgccagctc
     1201 cggcaggatt aagcgaactg gcgtgggtgc tttttggtat ttacctggcg gccattgtgg
     1261 gtctggttat caagcctttc ccggaacctg tcgtactgtt aattgccgtt gctgcctcaa
     1321 tggtggtggt cggtaactta tccgacggtg cgtttaagac caccgccgta ttaagcggtt
     1381 actcttcagg tactacctgg ctggtgttct cggcgtttac cttaagcgcc gcatttgtga
     1441 ccaccggttt aggtaaacgt attgcctatc tgctgattgg taaaatcggt aacaccacgc
     1501 tgggtctggg ttacgttacg gtattcctcg atctggtact ggctccggca acaccgtcta
     1561 acaccgcgcg tgcgggcggc attgtgttac cgatcatcaa cagcgtggcg gtggctttgg
     1621 ggtccgaacc ggaaaaaagt ccgcgtcgtg tcggacatta cctgatgatg tccatttaca
     1681 tggtcaccaa aaccaccagc tatatgttct ttaccgcaat ggcggggaac attctggcgc
     1741 tgaaaatgat caacgacatt ctgcacctgc aaattagctg gggtggatgg gcgctggccg
     1801 ccggattgcc gggcatcatt atgctgctgg tcaccccgct ggtaatttac accatgtatc
     1861 caccagaaat taaaaaggtg gataacaaaa ccatcgctaa agcgggcctt gccgaactgg
     1921 gaccgatgaa aatccgcgaa aaaatgctgc tcggtgtctt tgtgctggcg ctactgggct
     1981 ggattttcag taagtctctg ggggttgatg aatccactgt ggcaatcgtt gttatggcga
     2041 ccatgctgct gctgggtatc gttacctggg aagacgtggt taaaaataaa ggcggctgga
     2101 ataccttaat ctggtacggc ggtattatcg gattaagctc cttattatcg aaagttaaat
     2161 tcttcgaatg gttagctgaa gtctttaaaa ataacctggc atttgatggt cacggtaacg
     2221 ttgctttctt cgttattatt ttcctcagca ttatcgtgcg ttatttcttc gcttccggta
     2281 gtgcctatat cgttgctatg ttaccggtat ttgccatgct ggcgaacgtc tccggcgcac
     2341 cgttaatgtt aaccgcgctg gcactgttgt tctccaactc ctatggcggc atggttactc
     2401 actatggcgg cgcggcaggt ccggtcatct ttggcgtggg ttataacgat attaaatcct
     2461 ggtggttggt cggtgcggta ctgacgatat taaccttcct ggtgcatatc accctcggcg
     2521 tgtggtggtg gaatatgctg atcggctgga acatgctgta aatattctcg tcatacttca
     2581 agttgcatgt gctgcgtctg cgttcgctca ccccagtcac ttacttatgt aagctcctgg
     2641 ggattcactc gcttgtcgcc ttcctgcaac tcaaattatt tagagtatat ccatttatta
     2701 tctttctgcg cacttcacgg tgcgcagata tctggagcat ttgatgatca agttatctga
     2761 aaaaggcgtg tttctcgcca gtaataacga aataattgct gaagaacatt tcaccggcga
     2821 aattaaaaaa gaagaagcca aaaaaggcac tattgcctgg tctattctct cttcgcataa
     2881 tacgtccgga aatatggata aacttaaaat taagtttgat tcattagcct ctcacgatat
     2941 tacctttgtt ggtattgtac agaccgctaa agcgtccggt atggaacgtt tcccgctgcc
     3001 gtatgtgctg accaactgcc ataactcact ctgcgccgtc ggcggcacca ttaacggtga
     3061 tgaccatgtt tttggtttat cggcagctca gcgttatggc ggtatttttg tgcctccgca
     3121 tattgcggtc atccatcaat atatgcgtga gatgatggca ggcggcggca aaatgatcct
     3181 cgggtcagac agccacaccc gttacggtgc attagggaca atggcagtcg gtgagggcgg
     3241 tggtgagttg gtaaaacagc tgcttaatga cacctgggat atcgactatc cgggagttgt
     3301 tgcggtgcat ctgaccggaa aaccagcgcc gtatgtgggg ccgcaggatg tggcgctggc
     3361 tatcattggc gcggtgttca aaaacggtta cgtcaaaaac aaagtcatgg agttcgttgg
     3421 accgggcgtt agcgcgctct ctaccgattt ccgtaacagc gttgacgtga tgaccacgga
     3481 aaccacctgt ttaagctccg tctggcaaac tgatgaagaa gtgcataact ggctggcgct
     3541 gcacggtcgc ggccaggatt actgccagct taaccctcaa ccgatggcgt actacgatgg
     3601 ctgcatcagc gttgatttaa gcgccatcaa accaatgatt gcgctgccgt tccacccgag
     3661 caacgtgtat gaaatcgaca cactgaacca gaacctgacc gacattctgc gtgagattga
     3721 aattgagtcc gaacgcgtgg cgcacggtaa agccaaactc tcgctgctgg ataaagtgga
     3781 aaatggtcgc ctgaaagtgc agcaggggat tatcgcgggc tgttctggcg gtaactacga
     3841 aaacgtcatc gcggcggcga atgcactgcg cggtcaatcc tgtggcaatg acaccttctc
     3901 gctggcagtt tacccgtcat cacagccggt gtttatggat ctcgccaaaa aaggtgtggt
     3961 agcagatttg attggcgcag gcgcaatcat cagaaccgcg ttctgcggcc catgctttgg
     4021 cgcgggcgat acgccaatca acaacggatt aagtattcgc cacaccacgc gtaacttccc
     4081 gaaccgcgaa ggctctaagc cagctaatgg gcagatgtca gcggtggcgt tgatggacgc
     4141 tcgttctatc gctgcgactg cggcaaacgg tggctattta acctctgcca gcgaacttga
     4201 ttgctgggac aacgtgccgg agtacgcctt cgatgtaacg ccgtataaaa accgtgttta
     4261 tcagggcttt gtgaaagggg caactcagca accgctgatt tacggaccga acattaaaga
     4321 ctggccggaa ttgggtgcgc tgactgacaa tatcgtcctg aaagtgtgct cgaagatcct
     4381 cgacgaagtg accaccaccg acgaactgat tccttccggt gaaacctctt cttatcgttc
     4441 aaatccgatt ggtctggcgg agtttaccct gtcacgccgc gatcccggtt atgttggcag
     4501 aagtaaagcg actgctgagc tggaaaatca gcgtctggcg gggaatgtca gcgagctgac
     4561 agaggtgttt gcgcgcatta agcagattgc tggtcaggag catattgatc cgctgcaaac
     4621 tgaaattggc agcatggtat atgcggtgaa actaggcgat ggttctgcgc gtgaacaggc
     4681 ggcgagctgc cagcgtgtga ttggcggtct ggcgaatatt gccgaagagt acgcgactaa
     4741 acgctaccgt tctaacgtca tcaactgggg gatgttaccg ctgcagatgg cggaagtgcc
     4801 aacctttgaa gtgggggatt acatttacat ccctggcatt aaagcggcgc tggataatcc
     4861 gggtacgacg tttaaaggtt atgtgatcca tgaagatgcg ccggtaacgg aaattacgct
     4921 ctatatggaa agtctgactg ctgaagagcg cgagattatc aaggcgggta gtttgattaa
     4981 cttcaataaa aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc attcggcact
     5041 tattgtcgga tgcgatgctt gcgcatctta tccgacctac gaatcgcatc gaatctgtag
     5101 gccggataag gcgtttacgc cgcatccggc aaatagttaa tagctcttac ttcttcgcct
     5161 ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg ttgtagttag
     5221 tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta ttacccgcaa
     5281 acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa ccttcgttga
     5341 tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc agcgagcggc
     5401 ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg aggaaaccgt
     5461 agtaaatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga gtacgtgagt
     5521 tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa acgatatcca
     5581 catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacggttg gtttccagac
     5641 ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga atgttaacgt
     5701 tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg ttacctgcat
     5761 ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg ccattgtttt
     5821 gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg ctctggcagc
     5881 tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg ttgacgtcgt
     5941 gacgccagtc ggcagggctc atgcccccat caagggaaag cccaatcttc acatcaatcg
     6001 gtttttcacc tgtaccgtac agagtaattc cacccggagc agcagggaca tacaccgttc
     6061 cctgatactc accaggcatc acggcaatat actggcgctt gttagtgcgc ttgataattg
     6121 ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtaccc gccgggccga
     6181 caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct gcagcacctg
     6241 gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt gccgacagaa
     6301 tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg ggtggtgttg
     6361 agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg gaaactgaaa
     6421 atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg aagcctgctt
     6481 ttttatacta agttggcatt ataaaaaaac attgcttatc aatttgttgc aacgaacagg
     6541 tcactatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact ccctgcctct
     6601 gtcatcacga tactgtgatg ccatggtgtc cgacttatgt ccgagaagat gttgagcaaa
     6661 cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact cgtgaaaggt
     6721 aggcggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa aataccttga
     6781 tactgtgccg gatgaaagcg gttcacgacg agtagatgca attatggttt ctccgccaag
     6841 aatctctttg catttatcaa gtgtttcctt cattgatatc ccgagagcat caacatgcaa
     6901 tgttgtaggg atggcaattt ttacgcctgt tttgctttgt tcgacataaa gatatccatc
     6961 tacgatatca gaccacttca tttcgcataa atcaccaact cgctgcccgg taacaacagc
     7021 cagttccatt gcaagtctga gccaacatgg tgatgattct gctgcttgat aaattttcag
     7081 gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc gagtggcagc
     7141 gaccgggttt gttgttatat ggccttcagc tattgcctct cggaatgcat cgctcagtgt
     7201 tgatctgatt aacttggctg acgccgcctt gccctcgtct atgtatccat tgagcattgc
     7261 cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagccccc tccttattgc
     7321 tttaattttg ctcatgtaat ttatgagtgt cttctgcttg attcctctgc tggcgaggat
     7381 tttttcgtag cgatcaagcc atgaatgtaa cgtaacagaa ttatcactgt tgattctcgc
     7441 tgtcagaggc ttgtgtttgt gtcctgaaa
//
LOCUS       DAZFWP020000207         7434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFWP020000207 DAZFWP020000000
VERSION     DAZFWP020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.208"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            33..773
                     /gene="yiaT"
                     /locus_tag="ACHK0Z_RS22700"
                     /old_locus_tag="pgaptmp_004540"
     CDS             33..773
                     /gene="yiaT"
                     /locus_tag="ACHK0Z_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU4504430.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQVDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(720..983)
                     /locus_tag="ACHK0Z_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /pseudo
     CDS             complement(720..983)
                     /locus_tag="ACHK0Z_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            987..1088
                     /locus_tag="ACHK0Z_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /pseudo
     CDS             987..1088
                     /locus_tag="ACHK0Z_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1120..1815)
                     /gene="araD"
                     /locus_tag="ACHK0Z_RS22715"
                     /old_locus_tag="pgaptmp_004543"
     CDS             complement(1120..1815)
                     /gene="araD"
                     /locus_tag="ACHK0Z_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4504431.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1809..2669)
                     /gene="sgbU"
                     /locus_tag="ACHK0Z_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             complement(1809..2669)
                     /gene="sgbU"
                     /locus_tag="ACHK0Z_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGU4504432.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEMMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2662..3324)
                     /gene="ulaD"
                     /locus_tag="ACHK0Z_RS22725"
                     /old_locus_tag="pgaptmp_004545"
     CDS             complement(2662..3324)
                     /gene="ulaD"
                     /locus_tag="ACHK0Z_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4504433.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3321..4817)
                     /gene="lyxK"
                     /locus_tag="ACHK0Z_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             complement(3321..4817)
                     /gene="lyxK"
                     /locus_tag="ACHK0Z_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HGU4504434.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGGVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4821..5807)
                     /gene="yiaO"
                     /locus_tag="ACHK0Z_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             complement(4821..5807)
                     /gene="yiaO"
                     /locus_tag="ACHK0Z_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HGU4504435.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(5820..7097)
                     /gene="yiaN"
                     /locus_tag="ACHK0Z_RS22740"
                     /old_locus_tag="pgaptmp_004548"
     CDS             complement(5820..7097)
                     /gene="yiaN"
                     /locus_tag="ACHK0Z_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGU4504436.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFSIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(7100..>7434)
                     /locus_tag="ACHK0Z_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             complement(7100..>7434)
                     /locus_tag="ACHK0Z_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HGU4504437.1"
                     /translation="LTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFI
                     CGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNS
                     LTSPPQGA"
BASE COUNT         1736 a         2006 c         2004 g         1688 t
ORIGIN      
        1 tttttatctt attttaaaat ggaataccgg acatgttaat taaccgcaat attgtggcgt
       61 tatttgcgtt gccttttatg gcaagcgcaa ctgcttctga attatccatt ggtgccggtg
      121 cggcttataa tgaatcgcct tatcgcggtt ataatgaaaa tacaaaggca attcccctga
      181 ttagttatga aggtgattct ttttatgttc gtcagaccac gttaggtttt attctgtcgc
      241 aaagtgaaaa aaatgaactt agcctgaccg catcctggat gccgctggaa tttgaccctg
      301 ccgataatga cgatcatgcc atgcaacaag ttgataagcg tgatagtacg gctatggcgg
      361 gggttgcctg gtatcaccac gagcgttggg gaaccgtgaa agcctctgca gctgcggacg
      421 ttctggataa cagcaacggc tgggtggggg agctatcggt tttccacaaa atgcagatag
      481 gtcgcctgtc gctgacacct gcgctgggcg ttctctatta tgacgagaat ttcagtgact
      541 attactatgg catttcagag agtgagtccc gtcgtagcgg tctggcaagt tattccgcgc
      601 aggatgcctg ggtgccctat gtcagcctga cggcaaaata cccgatagga gagcacgtag
      661 tattgatggc gagcgccgga tacagcgagc tgccggaaga gattaccgac agcccgatga
      721 ttgatcgtaa tgagagtttt acctttgtca ccggcgtgag ctggcgtttt taattcaccg
      781 gtggatgtcg gtgcggctgg tgctccgtcg taatgccaca aaggttgttg ctctggattg
      841 ctctgttcat accgggaacc tggcgtctga atcaggatct tatggctgaa caatcccttg
      901 ccttcaaatg tcgtaaatgg cgcatcggcg ggcagcaaca ggttatgcag tgactcgacg
      961 ttgtcggcct gctccatgca atacggttta atgaggttgg ctatttccgg cagatttttc
     1021 gcaagcacac cggtttaacg ccagcagcgt ggaagcggcg ttacagcaag gaacatatca
     1081 attcgtagtg ccggggcgat gaagccccgg cgtgagggat tactgcccgt aataggcatt
     1141 ggccccatgc ttacgcaggt agtgcttatc cagcagttcg ttttgcatcg cagggagctg
     1201 cggcgcaagc tggcgcgaga ataggcccat ataggcgcat tcttcgagta ctacggcgtt
     1261 atgcacggca tcggcggcgt ttttacccca tgcgaacggg ccgtgagaat gcaccagcac
     1321 cgccgggatt tgtgccggac tcctgccacg ttcttcgaag gtttcaatga tcacttcgcc
     1381 ggtctgatat tcatattcgc cgttaatctc ctctgcggtc atctgtcgcg tgcaggggat
     1441 ggcaccatag aaataatcag cgtgcgtggt gccccaggcg gggagatcca gcccggcctg
     1501 cgaccagatg gtggcgtggc gtgagtgggt atgcacaatg ccaccgattt cggcgtagcg
     1561 acggtaaagc gccagatgcg ttggcgtgtc ggacgagggc tttttgctac cttccaccac
     1621 cttgccgctg gctatctcaa ccaccaccat atcgtcggcg gtcatcacgt catactcgac
     1681 gccggaaggt ttgattacca tccattgccg cgtttcgtct accgcgctga cattgcccca
     1741 ggtgaacgtc accagatggt gagcgggaag cgccagattc gccgccagca cgtcggcttt
     1801 cagttgctct aacatataaa tccagcctcc tgcatacgcg cttcaatcca acgccgcgcc
     1861 tgaataatct ccagcaccgg ctctttggct ttttcggtcc acatctcaat caggaaagaa
     1921 ccgcgatagt tcagtttatg cagcgttttg aagatgccaa cgaaatcgac gcagccttcg
     1981 ccaaacggca cgtcgcggaa ctgtccggga ctttgctcgg taactggctg ggtgtctttc
     2041 aggtggatcg cggcgatacg gtcaatgccc agtttcagtt cggcgggaac atcattgccc
     2101 caggcgctga ggttgccgac gtccgggtag acggtgaacc acggcgaggc gagcatctcg
     2161 tcccactttt tccatttact gatggagttc ataaacgcgg tgtccatcat ctccaccgct
     2221 agcatgactt gtgatgccgc cgcctgttcg accgcccacg ccagcccttc agcaaaacgt
     2281 tgccgggtgc cttcatcgtg gtcttcgtaa tagacgtcgt agcccgccag ctggatggtg
     2341 cgtatgccga gatcgcgtgc cagacgaatc gctttgctca tgatctcccg tgctcgctgg
     2401 cgtaccgcgt cgtcacggct accaaaagga aagcgacgat gtgcggacag gcacatcgac
     2461 gggatcccaa cacccgtttc gatcatcgcg gcaaccagcg aagtcctttg tgcggtgctc
     2521 cagtcaagac gtgaaagccg ttcgtcggtt tcatccaccg acatttcgac aaaatcaaaa
     2581 ccgcagcttt ttgccagcac cagccgctcc ggccaggaga gatctttcgc cagcgccttt
     2641 tcataaatcc ctaacggatg attacgcacg cttgcctccc cagatagcgt cgatttgcgc
     2701 atggaaatcg ccagccacct gcgccggatt tgctgagcct gccagtgctc gcccggcaat
     2761 aaacgctttc acgcgaatat ctttaaacag cggcaggtca gcaggggtaa tcccgccggt
     2821 aatggaaagc tcaaggccga tatcggaaag cgccttcatg cgtgccagat cggcttcgcc
     2881 ccactgttgc ccgctggcct gtgcatcacg accgcgatga taaatcgcct gccgcacgcc
     2941 aatacgatgc cagtcgcggg cgtcgtccag cgtccagtta ccgaacagct cgatctgaat
     3001 ttcaccaccg cagcgttgcg ccatcgcgtg gcctttttcc accgtcgcga gcggtgcggc
     3061 gcagatgatg gtcatccagt tagcgcctgc gccaaatgcc tgttgcgcga gcgtttcacc
     3121 agcgtcggcg accttccagt cggcaacgat gattttgtca gggcactgtt cgcgcaaggc
     3181 tttcaccgcg ccaagccctt cgtttaaaca gagaatggtg cccgcttcga caatatcgac
     3241 gctgtctttt aactgcgtca cgtcgcgctg tgcggcttca agtgatgagt ggtcgagtgc
     3301 cagttgcaga agtggtcggc tcataatatg tgctccttga tgcgggcgtg aaagccctga
     3361 agtgcggcaa tgagatgctg atagcgttgg tatttttgtt ggtaaagctg atgggcggtc
     3421 atatccggca gcagggtgcg taccgggtgc tgtaagtcac gttgagcttc gctgaaatcg
     3481 cgataaactc cggtgccgac acgggcggca agtgccgcgc caaaacagcc ggtttcttcc
     3541 acctgcggca gctcgatacg cagaccgctg acgtccgcca gcatttgcat ccagacatcg
     3601 gagtgcgccg ggccgccagt gacgcgcagg gtgtgcacat cagtaaaacg ttcgcgcatc
     3661 cggttgaggt gggtcatatg gctgaacacc cccccttcat aaatggcctg caacaggtgt
     3721 gcgcgggtgt gaatggcctg catcccgtag aaaccgctgg tcatctcgag tccggcgttg
     3781 ctgccgtaca ggaacggcag gaaaaagaga tcgccaccgg ccttcggcaa gctggcaacg
     3841 gcctggttga tctcatcaaa cgagatttct ccccactgtg cggtaaacca ttcgaggttg
     3901 ccggaagagg tggggctggc ttcgtgaacg ataaattcac catcgttaac gtagcgacca
     3961 tagacatacg gatgcgcttc accgtcacgt aaaccgcggg ttatgccgct ggtcaccgcc
     4021 caggtgccca tcaccgcatt aagggtaaat tcgtcttcga tcccggcgca gagtgcggtg
     4081 gaaaccacat caaacaggcc gccgacaacg ggcgtacccg ctttcagacc ggtcagtacg
     4141 gctgtctgag cggtgatctc cccgcagatt tcggcagatc cgacaacagg cggcagggcg
     4201 tgattgattt cagcaatccc cagccagtcg gtgaggcacg ggtcatattc cccaagactc
     4261 atgttgtaga gattggactc ggaaatattg ctctcttcac agcctttgac gccggttaaa
     4321 caccagcgca ggtagtcgtg cgtcatcatc acgcagccaa tttgcgcgta gcgttccggt
     4381 tcgtgctctt taagccaacg taacagcgat accggatgcc cggtccacaa ggtttgtcgg
     4441 gtcagcgggt agagtttttc cgggatgcca tcttcctgcc agcgacgaac gatttccatc
     4501 gcccggcgat ccgaggataa aatggcgtta ccgagcggct tgttgttttt atccagcaaa
     4561 aacaagcctt ttccttgtgc ggagataccg ataccgacaa tttgttcccc gctaacgccg
     4621 gaatgggtaa gcagttcgcg aatgacagcc atgcagcatt gccacagttc tgccatatcg
     4681 cgctctgccc aacctggctg cgggcttaat gcgcacagcg gcaggcgctg cacgcctgcc
     4741 tcccggcctt cgcggtcata cagcccggct ttcagccagc taccgccaca atctaacccc
     4801 agccagtatt gcgtcatggt ttattgcacc tcatccacgg ctttcagtag atcagcaccc
     4861 tgcggaacat cttttacaaa catgtcgcga acctgattac ccagagcgtc gctaaaggct
     4921 ttgcggtcga gatcggtgat gacttccacg cccgcttctt tcatgccgtc gatgattttt
     4981 tgctgatctt cggcaaccag tttgcgctgg tagttacccg cttcctgcgc agatgaaatt
     5041 agcgcttgct ggaactccgg gcttaagcca tcaaacttcg ctttgttgat caccaccaga
     5101 agcggggaat aggcgtggtg cgtcagagaa aggtacttct gcacttcgaa aaattttgct
     5161 gaccagacga cgttgatcgg gtgttcctga gcgtcgatag tgcgggtttc cagcccggta
     5221 tagacttcgg caaacggcat cgggatcggg ttagcgccaa agactttgaa tgcggcgata
     5281 ttcatcgggc tattgttggt gcgaattttc agccctttca ggtcggcggg ggttttaacc
     5341 ggtgcgcgcg agttggtgac atcgcgccag ccgttttccc agtaggccag tacttttagt
     5401 cctttacctt caagtgaggc tttcagatca tcaccgactt tgccgtcgag cgttttatgc
     5461 gcgtgagcgg tatcgcggaa caggaaaggg acatcaagca agttcatcac tggtgataac
     5521 ccggcaaagt tattcgagcc ggacatttcc atatcgatgg tgccgccacg tacgccgctg
     5581 atcatcgcct gcgcgttacc gagggtgcta tccggaaaca attttaattt cagctcgcct
     5641 ttggttctct cctgcaataa atcattgaat tttttcgccg caatatgttg cgagtcggtt
     5701 tgtgaggttt cataaccgaa acgtaaagat tgcgccgcca gagaagatgt gctgaatgca
     5761 gccaggccag caatgaataa tgcgtaggtt acagagcgta atttcataat attttccttt
     5821 taattaatcc atttcaaagg gaggatgatg agatcgggaa taaaaacaaa tacgactaat
     5881 aatgaatata aaaccaggac gtaagggaaa acgcctctga ctgcatcatc gaatttgagt
     5941 tttgccaccc ctgaaataac gttaagtaca ttaccgatag gcggtgtaat taagccgatt
     6001 gagcagttaa tgataaacat gacaccgaaa taaatcggat cgattcctgc ctctttaact
     6061 aaaggcatta ataccggggt aagaattaat accgtcggcg ttaaatccat gaccatgccg
     6121 acaatcagga tagccaccat aatgacgata aacagcagac gcggtgaatc gaccaacggt
     6181 tgcagtaaat cagaaaccat catcggcagt tcagcaatgg taatcagcca ggcggaaact
     6241 tgtgccgagg ccaccagaaa cataaccact gaggtggttt tcgccgcgcc aatgagtaca
     6301 tgccaaagtg tggcgaaggt catttcacgg taaataactg tggcgacaaa cagcgcatag
     6361 aaagcagcaa cggccccggc ttcggtaggg gtaaacagac cggagcgaaa cccgccaata
     6421 atgatgactg gaagaaacag cgcccagata ccggagacaa aagagtgcca gatttcctgc
     6481 atcgttgctt tttgctggcg gggtaaatta aggcgactgg cctgccacca ccaggtaagc
     6541 atcagcgttg cgcccatcat catgccaggg gcaatgcccg ccataaacag cttactgatg
     6601 gataatccgc tggaaacgcc gaagatacta aacggaatgg aagggggaat aattggcgcg
     6661 ataatgccgc cagaggcaat cagccccgcc gcccggttga ccgggtagtt ggcgctgcgc
     6721 atcatcggca ccagtagagc ggcaacggcg gcggtatccg caacggcaga accagaaagg
     6781 ctcgccataa tcatagctgc cagcacgccg acgtagccca gcccgcctgg tttatgcccc
     6841 accagtttca tcggcaggtc aacaatgcgt tttgacaagc cgcccgcatt catgatttca
     6901 cccgccagca caaagaacgg aatcgccagc agggagaagc tatcggctcc gttcaccagc
     6961 gtttgtgcca tgatctggac atcaaacatg tccagccaga acattaacgc cgccccgcac
     7021 aacaacagtg cccaggcaat aggcaaaccg atagcaatac cacccaacag acagcccaga
     7081 aaaatcagca cagccatgat taagctcctt gcggtggcga cgttagagaa ttgctacgcg
     7141 tgatgagttg atataaatga cgcagttcaa agaatgcgat aacgaggctg gtgggcagac
     7201 aagcggcata catcaggccg atgggtaaac cgaggatcgg tgaataatcg ctccagtcct
     7261 gaattgtttt tagcgttgcg ccccaggcca gtgcgccaca aataaataag attaaggaat
     7321 gggtaaccag agcgactcgt cgttgccatg cgggggagag tttctccacc agaaaggtga
     7381 cctgaacgtg ggcgttatcc ataaaagcca caatcgcgcc aataaatgtt aacc
//
LOCUS       DAZFWP020000208         7412 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFWP020000208 DAZFWP020000000
VERSION     DAZFWP020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.209"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(17..2002)
                     /gene="yjcS"
                     /locus_tag="ACHK0Z_RS22750"
                     /old_locus_tag="pgaptmp_004550"
     CDS             complement(17..2002)
                     /gene="yjcS"
                     /locus_tag="ACHK0Z_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU4504438.1"
                     /translation="MNNSRLFRLSKIIIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(2275..3204)
                     /gene="alsK"
                     /locus_tag="ACHK0Z_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             complement(2275..3204)
                     /gene="alsK"
                     /locus_tag="ACHK0Z_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HGU4504439.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(3188..3883)
                     /gene="alsE"
                     /locus_tag="ACHK0Z_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             complement(3188..3883)
                     /gene="alsE"
                     /locus_tag="ACHK0Z_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HGU4504440.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNKATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(3894..4874)
                     /gene="alsC"
                     /locus_tag="ACHK0Z_RS22765"
                     /old_locus_tag="pgaptmp_004553"
     CDS             complement(3894..4874)
                     /gene="alsC"
                     /locus_tag="ACHK0Z_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HGU4504441.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(4853..6384)
                     /gene="alsA"
                     /locus_tag="ACHK0Z_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /pseudo
     CDS             complement(4853..6384)
                     /gene="alsA"
                     /locus_tag="ACHK0Z_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
     gene            complement(6511..7410)
                     /gene="alsB"
                     /locus_tag="ACHK0Z_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             complement(6511..7410)
                     /gene="alsB"
                     /locus_tag="ACHK0Z_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HGU4504442.1"
                     /translation="MGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKKGIEDEAKTLGVS
                     VDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVARAWKKGIYLVNLD
                     EKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVAIIEGKAGNASGE
                     ARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIKAIYCANDTMAMG
                     VAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIGATGLKLMVNAEK
                     SGKVIPLDKAPEFKLVDSILVTQ"
BASE COUNT         1783 a         1884 c         1824 g         1921 t
ORIGIN      
        1 ccaaaagcga gtgacattaa tttggggtta cgatattcac ccacaaatcg aaattatcca
       61 gacaggcgat aatttcttcc agtttcgcgc cattgccaat aattttggct ttcttcgctt
      121 ttaccagatc cgccattttg gcttgcccgg tcagcacggc gtgcagatct tcacggctga
      181 tgtagaaaga ggcgtcagct tgggattgca gtgttttacg gtagttaagc acgctatcgt
      241 tcagcgtcag gttgaggtta tcgccattgc tcatattgaa gttcaggctg atatttttac
      301 ccgcggcttt cgcgctatcg aggcgaacgg acataaagtc gaacagcatt tcgaccgaca
      361 tcccgcgaat ggtgtccggg gaaccggtgg tgccgtggct gaacttatgc accccttcgc
      421 gcagctcttt cgcgccggtc aggtagaaac cgcgccaggt ggcggactcg gcctgatagc
      481 ccagctgttc aaagttattc gcttgcagat tcttcgcgac ctggtcaccc gggttggcgg
      541 caatcacctg tttcagcagt tctgccgacc agcggtaatc accttgcttg ttcgcttctt
      601 gcgccaggtt gatgacacgg gcagaaccgc ccagcgcctg cacgtaacgt ttacccatct
      661 ccacctgacc atacggatgc aggttagccg ggttaccgtc gtaatagcca agatagaagt
      721 tatacaccgc gcgggcgttg tggctgacag aaccgtaata gccacggctg gcccagttat
      781 tggcaagtgc aggcggcagt ttaatcatgt cgccgatttc attcatggtg tagccctggt
      841 tcgccaggtg cagggtctgg tcgtgaatgt acttgatagt atcgcgatat ttaccaatat
      901 aatcattgat atgcttattg ccccagaccg gccaggtgtg cggcataaac aggacttccg
      961 cgtcgttacc ccacatatcc agcgtttcgt tcagatactc ggtccacttg ctggtgtcgc
     1021 gggttttcgc gccgcgtaga gtgtagaagt tgtgcagggt atgcgtggcg ttctcggcgg
     1081 tacacagcgc tttcagggcc ggaatataga agtgcatttc ggctggtgct tcgctacctg
     1141 gggtcatcag gaagtcaaac tccaggccgt cgataatcat cttctcaccg gttttggtga
     1201 tggttttgtt cggtgcgatg atgctcgggt agcccgatgc cagcgttacg ccaagaccat
     1261 tgccgatgtt accctgaggg ttatgcgcca gcagcaggcc gtaggagtaa agtgcacggc
     1321 ggctcataat attacccgcc agtacgtttt cgctgatggc ttcgtccata aagccagcag
     1381 gggcgataac ctgcacttta ccggatttca catcggcttc agagataatg cctttcacgc
     1441 cgccatagtg gtcggtatgg ctgtgggtat aaataaccgc aacaatcggt ttttgcgggc
     1501 gattttggaa gtaaaggtca agggcggctt ttgcgctcgg tggcgtcacc agcgggtcga
     1561 tgacaataat cccggtatca ccttcgataa aggtgatgtt ggagatatcc tgaccgcgaa
     1621 cctggtacat tctgtcggtg actttaaaca ggccggaaat accgtttaac tgcgactgac
     1681 gccacaggct agggttaacg gtttcgggcg cgggcgcatt gatatcaaat ttataatctt
     1741 ccccgcgata atatggtttg ccattagcat cgcgcagaat accttcatcc agcagcggag
     1801 cgataaatcc acgctgagcg tcgtcaaaat cctgacgatc gctaaacggt aatgatttgg
     1861 cgtaattctg attaacctgt tgggtatatt gagtggcggc tttcgcttca tctgttgcgt
     1921 aagcggtatt taccatcatg ccgctggcgg cagttaacgc aataataatt ttgctcaaac
     1981 ggaataaccg agagttattc atataaatct ccatttatca tttatgtcgt aaatatgtaa
     2041 tccatgcaaa atacgtcatt taaagctggg gttatcataa ttgggaattt gcgactttaa
     2101 taagtggaag tgtgagctga acgatccatt ttattaggca tttgtgctga agaggaaaag
     2161 ttacagtaaa actatcattg cagaaaataa tatgagccgt tttatgtgtt tattatatat
     2221 attgtacctg tcgttggcac agcgggacat tgcgctactg tgccaacgct ttactcatgg
     2281 ggctttagca caggactgtg gcaaaaaatg ttgatgcgcc aatattgctg caccctgagc
     2341 gccattaaag tcagaagatg aggcggcaat aaagcgcacg acctgatgcg gcagtggacg
     2401 gcgcaggtac ttttgggtca tggcaatgag agtctcgcgt gggaaggcgg gcatatccat
     2461 cacgccaccg cccagaatca ccgcatcggg atcgaacaga ttaatgctgg tggcaatggc
     2521 ccgtgccgcg ttttcaagca gactctggac gaaaggggcg ttttccgcat ggacgaaaag
     2581 gtcgctcaat gggtaatttc ggggctgttg ttcgtaccag cgtcttagcg ccattccaga
     2641 gcaattggtt tccaggcacc caggattgcc acacgcgcag tgttgggtca tatctcccag
     2701 ggggatatga cccagttcgc ctgccacacc gtgtgcaccc gtccacggcg caccgttcat
     2761 ccacactgcg aaccccatcc ccgtaccgag ataggccgcc agaaccagtt gttgcgtaag
     2821 gcggttttct actacgtccc aggagagttg caggttaacg tcgcgggaaa actcaaccgg
     2881 acaattcagc gtattttcga gcttatcggc gagatcatat aaatccgccg ctgttaacgg
     2941 caggttaggc gtagaaatta tggtgcgttt atctttactg accagcgccg gaaatcccat
     3001 caccagacca tgacagcgag cgttaaagcg cctgagttgc tcgtcaatca tttcgccgat
     3061 acccgacacc aggccgggag caatgacttc tgcggtccgc tttttttcgc agtgtagcgt
     3121 ttcaccttct gctgtccgca gacaaaagcg gatatgcgtt gcccccatat ccacgcccgc
     3181 tacgacgtta tgctgttttt gcatgaggct gtacctcgct ttttgcagcc agaatctgcg
     3241 tggtcataat tctccatgct tcgtcgatat tttccgcatg attaaacagg ccggaagtgc
     3301 cgacgataaa gacatccgcc cctgccgcca tcagtttttc gtaagttgcc ttgttgcagg
     3361 aaccgtccac ctcaatttcg tactccagac cttctcgttc acgccatgcc ttcagttcgg
     3421 caagtttatc cagcatttca ggaatgaacg gttgtccggc aaagccggga tcgacagtca
     3481 tgaccgtaat tttatcggcc ttatggatat agtatttcat ggcctcaact ggtgtctccg
     3541 ggttaaggat cagccccact ttcatgtcat gacagcggat ttcatcaatc aggcggaacg
     3601 cctgaccgtt gatagtttcc ggatgcaaag tgatgaaatc agctcccgca cgcgccagtt
     3661 gagcaatgta atcctgcggc cgcgtcacca tcagatgaca gtcgagcggt ttagttgcca
     3721 gttttttaac ctgacttacg aagaacggtg agagtgtcag attggggaca aagtgaccat
     3781 ccatgatatc gatgtgaaag taatcggcat ggctgtcgat aaattcgatc tgttctttaa
     3841 atttcagcag atccatacac attaacgagg gggagatttt catgttcaat tccttactta
     3901 ctgataagac ggtcaagggc gacagccgcg ataattaatc cgcccatcac caccagttgg
     3961 taataggttt gtacctgcaa aatattcaga ccgttgttga tggtgccgat gatcaacccg
     4021 ccaatcacca cagagaaaat gcgccccttg ccgccgaaga aactggtgcc gccaatgatg
     4081 gcgctggcaa tggcataggt ttcaaaaccc ataccggcaa gcggttctgc ggcaccgagt
     4141 cgtgcagttg agacgacgcc tgccagacct gcacaaacac cagagatgat aaacaccacc
     4201 aggatgtgga atttcacgtc aatcccggaa tagaacgccg aatttttgtt gccgcccagt
     4261 gcgtagatgt tgcgcccgag ccgcatacgc gttgtcagaa accaaaggat gagcgcgaca
     4321 attagcgaga agataacggg gacaggtatc ccaattacgc tggcggcaaa gaagttcacg
     4381 aagtcaaagg agaagccgta taccgagttg gcatcggaga tcaccagcgt gatcccacgg
     4441 aaaatcgcgt tggtgccaag ggtgatgatg aacgggtgta gccccgtcca gttgaccagg
     4501 cagccgttga tcgcccccag tgcgccgcca accagtacac cgccaatcat cgctgcgaga
     4561 aacgggtcaa cacctgccaa catcagtttg gcggtcacca taccggaaag cgccagaatc
     4621 gcgccaaccg agaggtcgat accagcgacc aggatagcga aaaactcgcc catgccgatc
     4681 aataccgtca cggagctttg aacaaaaatc tgggtaatat tattggtggt cagaaaatat
     4741 tctggcgaca gcgaaccaaa aatggcgacg atgatcgcca ggataaaaaa ggtgccgtat
     4801 ttatcccaga acagcgcaaa gttgaacggt ttcttctcgc tcgcttcgct ttttactctt
     4861 gtggtaaagc ccatgccata atctcctctt cgctcatgtc atcgcgattc gtcaggattt
     4921 gcgtcagtcg tccttcgcag aacacggcga tgcggtcgca gacggtgata atttcaggta
     4981 gttcagatga caccatcagg atgacttttc cgtcgtccgc cagttggcgc atcactttgt
     5041 aaatttcggc tttcgcgcca acgtcgatgc cgcgggtagg ttcatcgaaa ataatcactt
     5101 ccgggcaaca gcacagccat ttggagatca ggactttctg ctgatttccc ccggatagtt
     5161 cggtgatatt ctggtttacc gaatgacatt tcagcgccag cagttcgcgt tgattttcag
     5221 cggtacgttg ctcgtcaact tcatgaaaca agcccatcgc gcctttatag ccgccgtctt
     5281 tcagactgcg gctgatcgcc atgttctgag cgatggaaaa gttgggaaaa aaaccgttat
     5341 cccggcggct ttcagtgatg taagccatcc cttttttcac tgcatccagg ggggaacgtg
     5401 gagagatatc tttgccatta agacggattt ctccgccagc gcgtttatca acgccaaaca
     5461 gacaattcat cagttcagta cgtccggaac cgaccagccc ggcaaagcct aatattctcc
     5521 ccggcagacg ctaaatgaga tatcccggac cttttttctg tcacggctgg tgacgttgcg
     5581 cacctcaaaa accgtatcgt gcacaaggtt gctgacattc tccttcatcg cgttaaaacg
     5641 gttttgcagt tcgcggccta ccatcagacg gacgatatcg tcatttgaca catcgcttac
     5701 catgccgctg caaacgctgc tgccgtcttt catcaccgta tagcggtcgc aaatacggcg
     5761 aatttccgcc aacttatgcg agatatagac gatggccgta ccctctttac gcaactgatt
     5821 catgatcaga aacagatagt ccacctcttt attggtgagt gaggaggtgg gttcatccat
     5881 gatgatgact ttggcatcga gcatcagcgt tttggcaatt tccagcatct gcttgtggct
     5941 gatagataga ttcgccactt tctcatctaa atcaactttc aagcccacgc gtaataacat
     6001 catggcggcg cggacacgca tttctcgcca gtcgataata ttgacgccac agattttttt
     6061 cgtcagatga cgaccaatat ataaattttc cagtacggtt aattcatcaa taacgctgag
     6121 ttcctgataa ataatcccga taccgagttg tgccgctaat ttatgatcca gcttgttata
     6181 gctaatgtta ttaatggtaa tggtgccttt ggtcggctca tgtattccgg ataaaacctt
     6241 cattagcgtg gatttacccg cgccattttc tcctagtaat gcatgtattt caccaggata
     6301 aaccgttaaa ttaaccgact ttaatgcgtg aaccggacca aaggacttgc cgatccccgc
     6361 catcgatata tatggcgtgg ccataaataa cctctctgca cgaaatatcg tattattgcc
     6421 tgatggctac atttatcagg tctacgttca ggttattttg ttggccggat aagacacttc
     6481 attagcatca tcatccggca tcattcagtt ttattgagtg accaggattg aatcgaccag
     6541 tttaaattcc ggtgctttat ccagcgggat aaccttgccg gatttctcag cgttaaccat
     6601 cagcttcaga cccgttgcgc caatatctgc cgggttctgc gcaaccgtcg cggtcatttg
     6661 tccggcttcc accattttgc gggcttccgg aattccatct gtaccgacga ccaatatttt
     6721 gcccgttttt ccggcgtttg cgactgcctg agcaacaccc attgccatcg tgtcattcgc
     6781 gcaatagatc gctttaatat tcggattacg ttgcaacacg ttagtggcga catccagtgc
     6841 tttaatgcgg tcccagtcag cgggctggct ggcgacaagc ttgatctggc ttgctttttt
     6901 gaaggcttcg gtggcaccat tacgacgcgc ttcaccggag gcgttgccgg ctttaccctc
     6961 aatgattgcg acttcaccac cttcagcgcc caatttgtca ataatgaacg acgcgccttt
     7021 cgccccgaca gcaacgttat cggtggtgac aaaagcttcc acattgccgc cagctttttt
     7081 cagattatcc atgtcgattt tttcatcgag attaaccaga taaatgcctt ttttccatgc
     7141 gcgggcgaca ggcatgacca gattcactga ggataatgga gcgaaggcga tacctttgta
     7201 atttttatta ctgagatctt caaataactg caattgagat tgaaaatcgc cttctgaagg
     7261 agaggcaaaa atatcaacgc tgacgcccag tgtttttgct tcatcttcaa tgcctttttt
     7321 catatctacc caaaatgggt tggagagggt tttcaatacg acagcatatt cggcggcagc
     7381 aaaagcgctg gttgacaaca ttaagcccac ga
//
LOCUS       DAZFWP020000209         7206 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFWP020000209 DAZFWP020000000
VERSION     DAZFWP020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.210"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(190..1383)
                     /gene="tyrB"
                     /locus_tag="ACHK0Z_RS22780"
                     /old_locus_tag="pgaptmp_004556"
     CDS             complement(190..1383)
                     /gene="tyrB"
                     /locus_tag="ACHK0Z_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU4504443.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1636..2715)
                     /gene="alr"
                     /locus_tag="ACHK0Z_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             complement(1636..2715)
                     /gene="alr"
                     /locus_tag="ACHK0Z_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU4504444.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGIIKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2768..4183)
                     /gene="dnaB"
                     /locus_tag="ACHK0Z_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     CDS             complement(2768..4183)
                     /gene="dnaB"
                     /locus_tag="ACHK0Z_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4504445.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4266..5249
                     /locus_tag="ACHK0Z_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     CDS             4266..5249
                     /locus_tag="ACHK0Z_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU4504446.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5415..5657)
                     /gene="pspG"
                     /locus_tag="ACHK0Z_RS22800"
                     /old_locus_tag="pgaptmp_004560"
     CDS             complement(5415..5657)
                     /gene="pspG"
                     /locus_tag="ACHK0Z_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU4504447.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5791..6828)
                     /gene="dusA"
                     /locus_tag="ACHK0Z_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             complement(5791..6828)
                     /gene="dusA"
                     /locus_tag="ACHK0Z_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU4504448.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1832 a         1909 c         1764 g         1701 t
ORIGIN      
        1 actccaccca aaaaaacagg ataaggaatt ccagataatt aggataagct taacaataaa
       61 agaactaata acgtttatta tctctcacgt agaacgatgg catcaaaaaa atcatctata
      121 aagaaagagt gaaagcgacg acagtttaat ttcactgcag gctgggtagc tccagcctgc
      181 tttcctgcat tacatcaccg cagcaaacgc ctttgccaca cgttgtacat ttgccgtatt
      241 taacccggcg acacacatgc gaccgctggc gatgagatag acaccaaatt cttcacgtag
      301 tcggtcaacc tgagcggcac ttaaaccggt ataactgaac atgccgcgct gattaagcag
      361 ataatcgaaa ttgcgttctg gcatctctgt gcttaatacc ttcaccaatt cctgacgcat
      421 tgccagaatg cgagtacgca tctcttctac ttccgccagc cagctggctt tcaatgcctc
      481 gtcattcagc actgcagcca ccacctgcgc accaaaattc ggcgggctgg agtagttgcg
      541 gcgaactgtt gctttcaatt gccccagtac gcggccagcg gcttcggcat cttcacacag
      601 aacagaaagt ccgccgacgc gctcgccgta aagggagaaa attttcgaga acgaattgct
      661 caccagagcg ggtaatccag cgctggcaat ggcgcgaata gcgtaggcat cctcttccat
      721 accggcacca aatccttgat aggcaatatc gaggaatgga ataagctcgc gggctttgag
      781 aatttcaatc accgcatccc actgatcatt agtgagatcg gcacccgttg ggttgtggca
      841 acatggatgc agcaacacaa tactgcgggc aggtaatgtt ttcagcgtcg ccaacaggtc
      901 attaaagcgc acgccgttag tcgcttcgtc ataccagggg taagtactca cttcgaatcc
      961 agccccggcg aatattgcta cgtggttttc ccaggtagga tcgctgaccc agacgcctga
     1021 ttccgggaag tagcgtttca ggaaatccgc gcccactttc aatgcccctg agccgccaag
     1081 ggtttgaatg gttgctacgc gctgttgttg cagtactgga tggtccgcac caaacagcag
     1141 cggcgcaatg gcatggcgat agctgttaag cccttccatc ggtaaataaa gcgaagcgcc
     1201 atgaggctgc gcattcaggc gcgcttccgc ctccgccacg gcttgcagtt gtggaataat
     1261 tccgtcttcg ttgtagtaca gaccgatact taaattcact ttgtcgctgc gagggtcttc
     1321 tttaaaacgc tccataagcg taagaatcgg gtcgccagcg taggcgtcaa ctttttgaaa
     1381 cacgcgatgg ttctccaggt ttacgggcag gtggttaaaa cacaataaac cggaagaagg
     1441 cgaagatcga gtggatgttc aggagcgaac ggcaattagc aacagagtga gactcatgac
     1501 aaacgtacat ccgccagagg cacgaccttt ataagagcat gaaagaacat caacttattg
     1561 aatttttagg attttattgg ccggataagg cattcacgcc gcatccggca cagacaatca
     1621 aatattacag aacgattaat ccacgtattt catcgcgacc cttgaagtca ggcgcgtaat
     1681 aagttcgtaa gcgcttactt tcgtcatttc agcgatacgt tctacgggca aaccttcgcc
     1741 ccataaaatg accggatccc cggctttgtc ctgcgcttgt ggacctaagt ctacgcagat
     1801 catatccatc gccacgcgcc cgacaatcgg tacttcgcga ccgttcacca gcactggcgt
     1861 accggacggc gcggcgcgcg gataaccatc gccatagccc atcgcgacta cgccaagacg
     1921 ggtatcacgt tcgcttaccc aggttccacc ataaccaaca ggttctccgg ctttatgctc
     1981 acgcacggca atcaggctgg aggttagtga catcactggc tgacagccaa aatcggcacc
     2041 ggtggagcga tcttccagcg gcgagacgcc ataaagaatg atgcccgggc gcacccagtc
     2101 aaaatgcgac tgtggccaca gcagaatgcc acccgatgcg gcaatggaac gttgaccagg
     2161 tttgccttcg caaaaggtat taaagatagc gagttgtttc tcggttgcgc cacattttgg
     2221 ttcatccgcg cgcgcaaaat ggctgacgat attcaccggc tgacgaacgt ttttgcactg
     2281 ggtcaggcga tgataaaacg cctcagcctg ttccggcctt acgcccagac ggtgcatacc
     2341 ggtatcgagt ttcatccaga cggtaaccgg ctcgtccagg ctagcctctt ccagcgcagc
     2401 cagctgttct tcgttatgca cggcggtatg aaaatgttgc gcagaaatcg tcggcagatc
     2461 tctggcatca aaaaagcctt cgagtaacag tacaggtttg atgattcccc ccgcacgcag
     2521 tcgcagagct tcttcgagac gggctacgcc aaaggcgtca gcatcgggga gcgttcgcgc
     2581 ggtctcaaga agaccgtgac cataagcgtt cgctttcacc accgcaacca ttttactggc
     2641 aggggccagt tcacgaagac gttgcaggtt gtgtcgcaga gcgcggcggt taatcacaac
     2701 agttgccgct tgcatttgtg ttccttgata agtgtttgct ttaattacct aattcataaa
     2761 ataattatta ttcgtcgtcg tactgcggcc ccgcatagtt gtcgaagcgc gaccattgac
     2821 cgttaaaggt caggcgtacc gtcccgattg ggccgttacg ttgtttaccg ataataattt
     2881 ccgcgatgcc ttttaaatca ctgttttcgt gatacacctc atcacgatag ataaacatga
     2941 tcaagtccgc atcctgctcg atagagccag attcacgcag gtcggagttg accgggcgtt
     3001 tgtcggcacg ttgttccaga gaacggttca actgggacag cgccaccacc ggcacgttca
     3061 gttctttcgc cagtgctttc agcgagcgag agatttctgc aatttccagc gtacggttat
     3121 cggaaagcgc cggtacgcgc atcagttgca ggtagtcgat catgataagc ccgatgccgc
     3181 cgtgttcacg ggcaatacgg cgtgcgcggg aacgcacttc cgttggcgtc aggccggagg
     3241 agtcatcgat ataaatattg cgtttttcga gcaaaatacc catggtgccg gaaatgcgcg
     3301 cccagtcttc atcatcgagc tgcccggtac ggattttagt ctggtcaacg cgcgacagcg
     3361 acgccagaga acgcatcatg atctgttctg atggcatctc cagcgagaag ataagtactg
     3421 gtttatcctg caacatcgcc gcgttttcga cgaggttcat cgcaaatgtt gttttaccca
     3481 tcgacggacg cgcggcgacg atgatcaaat ccgacggctg caagccagcg gtttttttgt
     3541 tgagatcgtc ataaccggtg tttactccgg taacgccatc gtgtggctgc tgaaacaact
     3601 gctcaatacg cgccacggtt gcgtcgagca catcggcgat gttcttcggc ccttcgtctt
     3661 tgtttgcacg actttcggca attttaaaga cgcgggattc agcaaggtcc agcagatctt
     3721 cgctggtacg cccctgcgga tcaaaaccgg cttcagcaat ctcattcgca accgagatca
     3781 tctcacggac aacggcacgt tcacgcacga tgtcagcata agcactgatg ttcgccgcac
     3841 ttggcgtatt ttttgacagc tctgccagat aagcaaaacc accgacgcta tcgagttgcc
     3901 cctggcgttc cagcgattcc gcaagggtaa tcagatcgat aggactaccg ctttcctgca
     3961 aacgcgccat ttcagtaaag atatggcggt gtgggcgggt gtaaaaatcg tctgccacca
     4021 cacgctcggc tacatcgtcc cagcgttcgt tatccagcat taaaccgccc aacaccgact
     4081 gctccgcttc gatcgagtgc ggaggcactt tcagcccggc aacttgtgga tcgcgttcgc
     4141 ggggttcagc ctgctgtttg ttgaagggtt tatttcctgc catagtgaat ggagttaccg
     4201 agataaagaa tgggtcgaaa ctttaccata tgaagcagac cctgacgata cgttctggag
     4261 gacacatggc aacacgaatt gaatttcaca agcacggtgg cccggaagta cttcaagccg
     4321 tagagttcac tcctgccgat ccggcagaga atgaaatcca ggtcgaaaat aaagccatcg
     4381 gcatcaattt tatcgacacg tatatccgca gcggccttta cccgccgcca tcactaccca
     4441 gcggattagg taccgaagcg gcaggcatcg tgagtaaagt cggcagtggt gtaaagcata
     4501 ttaaggcagg cgatcgtgta gtctatgcgc agtcggcgtt aggcgcttac agctctgtgc
     4561 ataacattaa tgcggataaa gcggcgattc tgcctgcggc aatttctttt gagcaagctg
     4621 cagcatcttt cctgaaaggc ttaacggttt attatctgct gcgcaaaacc tatgaaatta
     4681 aacccgatga gcagttcctg ttccacgcag cagctggcgg cgttggcttg attgcctgcc
     4741 agtgggcaaa agccctgggc gcgaaactta tcggcaccgt aggaaccgcg caaaaagcgc
     4801 agagcgcgct aaaagcgggc gcgtggcagg ttattaacta tcgtgaagag gatctggtcg
     4861 agcggttaaa agagatcacc ggcggtaaga aagtgcgcgt ggtgtacgat tccgtgggca
     4921 gagacacctg ggaacggtcg ctggattgcc tgcaacgccg cggcttaatg gtcagttttg
     4981 gcaactcatc aggtgcggtt accggtgtga acttaggcat tctcaatcaa aaaggctcgt
     5041 tgtatgtgac acgcccttcc ctgcaaggct atatcaccac gcgggaggaa ttaaccgagg
     5101 ccagtaatga actgttctct ttaattgcca gcggtgtgat taaggtcgat gtcgccgagc
     5161 agcagaaata tccgctgaag gatgcgcagc gtgcgcatga gattctggaa agccgggcga
     5221 cgcaaggttc cagcctgttg attccataaa agaaataggg cttccacctg ggaagccctt
     5281 tctttttata gttcggctgt atgtagggta cagcacgatg aatctgttag aggcgcaata
     5341 gtgacagatt tgattatcaa ttcctatttt gttctaagga taaaacctta ggttgtgatc
     5401 atccgcacaa tcccttagta acgccagcgg tcataacgct gatatttcgg cacttttggt
     5461 gctttaatcg ccttaataac ccacaccacc gcaatcgcca gtagtaacca cggcagcaac
     5521 ttaatcatca atgccagcat accgccgagg aacataatgg ccgtcgccac aaccagcgcg
     5581 gcgataatgc ccagcaacga aacgccggtg accatcagca tgacaaaaaa gccaatcaca
     5641 aaaagtagtt ccagcatgat gctctcccaa atatgaaatc tcttgctggc attacaagaa
     5701 tcatgccaaa aataatctat tgatttaaca gcaaaacgcc ccgcgacggt gcgcagggcg
     5761 tggtgaattt gactactttt tggtgaaaag ttaacgctta tccgccacca gtttgagcgc
     5821 gtgttccagc acattaatgt ctgcacccgc tttatgggca ttttcactta aataacgccg
     5881 ccactgccgc gcgccaggaa taccctggaa caagcccaac atatgccggg taatatggcc
     5941 gagatacgtc ccctggctga gttcacgctc aatgtacgga tacatggcgc gcactaccgc
     6001 caccggatcg gcatcggtat ccgaggaacc aaagatctcc cggtctactg ccgccagaat
     6061 acccggattc tgatacgcct cgcgcccgac catcacgcca tccatatgtt gcaggtgtgc
     6121 tttggcctct tccagcgact tgataccacc gttaatcgac attgtcagat gcggaaagtc
     6181 acgcttcagt tgatacacac gcggataatc gagcggcggg atttcacggt tttctttcgg
     6241 gcttaacccc gaaagccagg ctttacgtgc gtggatgata aacatctcac actcgccttt
     6301 gccggaaaca gtgttgatga aatcgcagag aaattcatag ctgtcctggt catcgatgcc
     6361 aatacgcgtt ttcaccgtca ctggaatcga caccacatcg cgcatcgctt tcacacagtc
     6421 ggcaaccagc tgcgcattac ccatcagaca cgcaccaaac atgccgttct gcacccggtc
     6481 agacgggcag ccgacattca ggttgatctc atcatatccg cgcgcttctg ccagctttgc
     6541 acactgcgcc agcgccgccg gatcgctacc gcccagttgc aacgctaccg gatgttcttc
     6601 ttcactgtac gccagataat cacctttacc gtgaataatc gcccctgtgg tcaccatttc
     6661 ggtatacagc aacgtattgc gggaaagcag acgcaagaaa tagcggcaat gtctgtccgt
     6721 ccagtcgagc attggtgcaa cgctaaaccg accactccag tgaacgtcag ttttttcagg
     6781 cattgcgctg gtttggttga ttttttgcat ttcagaatta ccgtgcattt tcaaatgtag
     6841 agattatttt atcgatatat catggggtta tgttattcag catcactatt caggaggctc
     6901 aatagcgggg tactatacca taacaacagg aagcgcctgt ctcattgcaa aagaaaattg
     6961 agatcctctc aaggcatgaa gctctcacga agtgatggaa ataatcttat tagccgttaa
     7021 ctttgttaag gccaatgata aacaatccag gttcgacgat aaataaaaaa tcacacatta
     7081 aactctggtg atatatctcc ctgctaatag cattgataga gaaaaaagaa cccaataaat
     7141 attgggtcct tttatataat gccttccata ctatcgaaga acttcacata ttatttctcc
     7201 gattta
//
LOCUS       DAZFWP020000210         7186 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFWP020000210 DAZFWP020000000
VERSION     DAZFWP020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.211"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(60..1652)
                     /locus_tag="ACHK0Z_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             complement(60..1652)
                     /locus_tag="ACHK0Z_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU4504449.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHK0Z_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHK0Z_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4504450.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /locus_tag="ACHK0Z_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             complement(2850..3968)
                     /locus_tag="ACHK0Z_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU4504451.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLCAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHK0Z_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHK0Z_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU4504452.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHK0Z_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHK0Z_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU4504453.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHK0Z_RS22835"
                     /old_locus_tag="pgaptmp_004567"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHK0Z_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4504454.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHK0Z_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHK0Z_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU4504455.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         1813 a         1940 c         1705 g         1728 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actggctcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctgag catagtaccc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaagttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcag ctcagcaatc tgcgctttct tcttggcgtt atcagccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttgcc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaaa ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt attggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccact aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgcaca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt ccactcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtgtcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaatttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggtcaaa tcggcgaata
     5941 cgataccgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattca
//
LOCUS       DAZFWP020000211         7123 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFWP020000211 DAZFWP020000000
VERSION     DAZFWP020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.212"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(319..1455)
                     /locus_tag="ACHK0Z_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             complement(319..1455)
                     /locus_tag="ACHK0Z_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4504456.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAVALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1458..1820)
                     /locus_tag="ACHK0Z_RS22850"
                     /old_locus_tag="pgaptmp_004570"
     CDS             complement(1458..1820)
                     /locus_tag="ACHK0Z_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4504457.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTVGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHK0Z_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHK0Z_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU4504458.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4365..5513
                     /gene="mtlD"
                     /locus_tag="ACHK0Z_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     CDS             4365..5513
                     /gene="mtlD"
                     /locus_tag="ACHK0Z_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4504459.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5513..6100
                     /gene="mtlR"
                     /locus_tag="ACHK0Z_RS22865"
                     /old_locus_tag="pgaptmp_004573"
     CDS             5513..6100
                     /gene="mtlR"
                     /locus_tag="ACHK0Z_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU4504460.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6112..6321)
                     /gene="yibT"
                     /locus_tag="ACHK0Z_RS22870"
                     /old_locus_tag="pgaptmp_004574"
     CDS             complement(6112..6321)
                     /gene="yibT"
                     /locus_tag="ACHK0Z_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU4504461.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6606..6968
                     /locus_tag="ACHK0Z_RS22875"
                     /old_locus_tag="pgaptmp_004575"
     CDS             6606..6968
                     /locus_tag="ACHK0Z_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU4504462.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1814 a         1787 c         1804 g         1718 t
ORIGIN      
        1 tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc atcatcgcaa
       61 tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg aacaactcat
      121 atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa gacggataaa
      181 aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt aaagaaaaat
      241 catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag caaagttcca
      301 taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc tggtcattct
      361 tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg
      421 ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      481 cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg
      601 tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca ccggacgcag
      721 cggcaaggca actgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct
      781 cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca agtaacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg
     1381 atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1441 aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt tttctcggcg
     1501 gcgagttgat gctccagccg ggaaattcgg tcagaaagtg cggcgatttc cgggtcagtc
     1561 gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc
     1621 gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct cacccagccc
     1681 accgtatgaa tagcgtcggc atggggatgg ttgcgctttt tggcaatcag ataagggatg
     1741 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1801 gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gtttttgggc
     1861 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1921 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattgtat
     1981 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     2041 attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta ctcacattta
     2101 aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg ttttgtgatg
     2161 aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc acacaaaagg
     2221 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2281 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2341 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2401 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2461 gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat
     2521 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2581 acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg cagacatgcc
     2641 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2701 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2761 aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc cgattgttga
     2821 agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2881 gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2941 cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat caatcttctt
     3001 cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg
     3061 tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc tggggggtat
     3121 ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct
     3181 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc
     3241 atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt atttcgctaa
     3301 catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa
     3361 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3421 gaaagctgag tctaaaggca catctccgct gtctgcgggc gatgtgacta acgacctgag
     3481 ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg cgatgggcgc
     3541 aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca cagatttcgg tcactaacag
     3601 cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg acctgaccga
     3661 acgcgctatg cgccaggttc cgcaggcgca gcatatttcg ctgaccaact tcctcgacag
     3721 cggcctgtac accagcctaa ccgaacgtct ggttgctgcc cagcgccata ctgaaaacga
     3781 agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3841 actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag aagcgattcg
     3901 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat
     3961 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     4021 tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga
     4081 aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg gtattgcagc
     4141 ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgagtc
     4201 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgttggcagg
     4261 tcgtaagtaa tccaatccta ccctctcctc acggagaggg tttgggtgag ggaaaagcct
     4321 caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa gcattacatt
     4381 ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac gcgggtatcc
     4441 aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct
     4501 atcaggttca tgtggttggt gaaaccgagc aggtagatac cgtttccggc gtcgatgctg
     4561 tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggtcgattta gtcactaccg
     4621 ccgttggccc ggttgtgctg gaacgtattg ctccggctat cgccaaaggg ctggtgaaac
     4681 gtaaagaaca aggtaatgaa tccccgctga acatcatcgc ctgtgaaaac atggtacgcg
     4741 gtaccacgca gctgaaaggc catgtgatga acgccctgcc ggaagacgcc aaagcgtggg
     4801 tagaagaaca cgttggcttt gtcgattccg ccgttgaccg catcgtaccg ccttcggctt
     4861 cggcaactaa cgatccgctg gaagtgacgg tagaaacttt cagcgaatgg attgtcgata
     4921 aaacgcagtt caaaggcgca ctgccgaaca tcccaggcat ggagttaacc gacaacctga
     4981 tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg tcatgctata accgcgtacc
     5041 tcggaaaact ggctggtcat cagaccattc gtgacgctat tctcgacgag aaaatccgcg
     5101 cggtggtaaa aggtgcgatg gaagaaagtg gtgcggtact gatcaagcgc tacggctttg
     5161 acgcagacaa acatgcggcg tacatccaga aaatcctcgg tcgttttgag aacccgtatc
     5221 tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagt gctggcgacc
     5281 gtctgatcaa gccactgctc ggtacgctgg aatacagcct tccgcacaag aatctgattc
     5341 aggggattgc tggtgcaatg cacttccgca gtgaagatga tccgcaggct caggaactgg
     5401 cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc ggtcttgatg
     5461 ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa taatggtgga
     5521 ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg caacaatgga
     5581 acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg
     5641 aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca
     5701 ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac tcgacggcga
     5761 cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa
     5821 ccgccaggaa tacgaagatg cggagctgct gatggcattg cgtgaagaac taaatcacga
     5881 cggcaacgag tacgccttta ccgacgacga gatccttgga ccctttggtg aactgcactg
     5941 cgtggcggcg ttaccaccgc cgccacagtt tgaaccagca gactccagtt tgtatgcaat
     6001 gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac tgactgagct
     6061 gatttccaga atcagcttaa aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc
     6121 ctctacctgc ttcggccgat aaagccgacg ataatactcc agacgttgta gatataacgg
     6181 cacgctttca tcgggtattc cggacggaat cgcgttacgg ggaggaagtt ttttcagata
     6241 ctcccggaac gcctggcttg atgccatgaa atctacggct ttatcgataa gaagcggaac
     6301 gttttcacct aatttgccca tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg
     6361 ccgccaattt tagtttaggt tcccggtaaa gatgacatta aggaaaacgt gctgaatcct
     6421 caataatcat ccgctgctgt ttattatctt ttttctatag atttcttaat taatcaataa
     6481 attgcgtagt ttttaagcat gttaatgatt acgttacaga actattgcaa tgtcatcagt
     6541 tgtgaacaaa gcacctggtc gcgcatacta ggggctataa atttatcttt atcagaagcc
     6601 atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct cagcgatcgt ctggacgcca
     6661 tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc agacaaatat gccgagctgg
     6721 aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct gcgtgaagtt catagccaaa
     6781 aactgagtaa agaagcacag aagctgatga agatgccgtt tcagcgcgca attaccaaaa
     6841 aagagcaggc tgatatgggc aagctgaaga aaagtgttcg cggactggtg gttgtgcacc
     6901 caatgaccgc gctgggccgt gaaatgggcc tgcaggagat gactggattc tctaagactg
     6961 cgttttaagc gcctcagacg actggttcga tcatatcaac ccgcatctgg cttgccttag
     7021 ctggtcaatg cggggactct cctgtctact cgcctaatta ccaaacaatt cactcccttt
     7081 acttcgattt accgctggtt gatattctga aaatgcattt tta
//
LOCUS       DAZFWP020000212         7005 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFWP020000212 DAZFWP020000000
VERSION     DAZFWP020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.213"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(13..2796)
                     /locus_tag="ACHK0Z_RS22880"
                     /old_locus_tag="pgaptmp_004576"
     CDS             complement(13..2796)
                     /locus_tag="ACHK0Z_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4504463.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMFETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRTT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     YPVNTYTGVLLPK"
     gene            complement(2853..5225)
                     /locus_tag="ACHK0Z_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             complement(2853..5225)
                     /locus_tag="ACHK0Z_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4504464.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5263..6948)
                     /locus_tag="ACHK0Z_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             complement(5263..6948)
                     /locus_tag="ACHK0Z_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU4504465.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1811 a         1608 c         1574 g         2012 t
ORIGIN      
        1 acggggctgt tattattttg gcaataatac tccggtataa gtatttaccg gataagaaag
       61 atattgttta acggcagtgt taacattctc taccgtcatt tgtttcaaca attgctcctg
      121 ctcagtccat gctgcaggat cgtcatattg aataagacta tttacaatag tgttcgctaa
      181 ttgttgaacg ctacgctgtt ggatatcgag gctgcgctga acgttttgct ggtattcatt
      241 cagttcttgc tcactgatcc ctttagccag acgcttaacc atcacttcat tcgctaacgt
      301 taacagttca tcatgtcgtt ctggttgaca agtaaaagcc agcaaatgac tgatatcttt
      361 ggcctgagga tcaaccgaga ggcgagaaga aacgctgtat gctccagatg cctgttcacg
      421 aatattaaca cgtagatctt ttgccagtgc gacgttaaaa gcatcgagcg ccatacgcgt
      481 cgccagatta acaggtgtcc gggaatcata acgcttccac tgtgaaacct gtgccacagg
      541 ttcattttgt tcttttacag taaccgatgc gttgtccgtc gtgcgagtta atggtttacc
      601 tgcggctaat ggcgaatcag agtgtttgat tgatcctaag taacgcgtaa ttaacgccac
      661 gagtttgtct tctgagacat taccgacaat gacaaacgtg atatccgctg gagatgaaaa
      721 caactggcga tcggcagcca gcgcatctgc ggcagtaaac tgtacaatct gattttcttg
      781 cagtaatttc gtgcggtcat cagcatagcg cgtctcaaac atctgctggg cgaatttctc
      841 cgctggacgc tggtcgagcg ttttcaacgc ctgaatttga gcattttgta gcgatgccca
      901 aatattgtcg ttaatcgtgc tgtgggtgat tcgctggtta atcaactgaa aaccaggttc
      961 agggttatta gtccgcgcgc taacggagag caacgtattc atgccactga ctttactgct
     1021 catggttacc gaattttccg cactccagcg tttcaggctg gaagaggaga gctcaccaac
     1081 gccgcttccg cttactgctt tatttgccag cgcgataagt gatttttgct gcgcagggaa
     1141 acttaaatcg cctttattcg atacggcagt aatttgtagc ttttgctctt cacccgcgga
     1201 ttttgccaga ataaccctgg caccattgga aagtgttaat gatgtcagat tctcagccag
     1261 cgtttctttg ctgctaattt ccgcctgtgg gtcagcgtct actgttaacg ataaatttct
     1321 gcctgggaag atgtaagccg ccagcttttt gttggcgtac tctttttcca gcgccaggat
     1381 agccgcagga gacaatgctt ttttggcggc aagctcattg tttaccattt gctcccaaaa
     1441 tgcgtcctgg ttctttctta actgctgcca tttttccgcc agactttgca cggtaatttg
     1501 ctgccacaga cgtttcgaaa gttgatatgt ctcttccggc gacaagaacg gcgtattatt
     1561 taatgagctg gatgccaggc gactggtcag catacgtaaa tcacgttcag cttgctgatc
     1621 aaccgcattt ttcagccagg tgaggcgggt agatttgaca tcatcgagtt cttcagcaga
     1681 aaaaccatgc tgatcaatgg ttgccaactc tgccattaat gcattcgcag catcctgcat
     1741 attatcgtct cgtgcattta cacggaaaaa cagcgactga taatcgggtg caattttaac
     1801 gctgcgcgca gtgccgccag aaatagtctt caactcgccc gactgtatgc gttcctgcag
     1861 acgttgattg aacagctgaa ctaacatgct ccattcagct tgttcgataa agctttgctc
     1921 atcgttcact tgtaccattg gcaggcgata atagagtgcg atgccgttca cccggttttc
     1981 tttatcatta atgatattaa agcgcaggtg gttttcggct tttgtcggcc agacgcgatt
     2041 ttcagctgct ttgttagccg gaagcttact taaattatcc tttatcagcg ccagcgcttc
     2101 tttactgtcg atatcgccga ccacgataaa ggtcatatta tttggttgat accagcgttg
     2161 ataaaattgg cgcaattgtg ccggtgtgac cgtggcgaca gtatccatca ggccgatagg
     2221 ttcacggtct aaattacggg tattagccag caggaaaggg cggcgcgcct gagaggtgcg
     2281 ccatttcgca tcctgatggg cacgccattc ctcagtaatt acgccacgtt cagcgtctac
     2341 ttcgagtttt tcaaaggttg cggcattact ccattcactg aagattgcca tcacttgttg
     2401 cagattttgt ttctgcgtag tcggcaaact cacctgatac accgtttcgt catagctggt
     2461 ataggcatta acatcgcgac caaaacgcag gcccattgac tcaaatgttt cgatgacttt
     2521 attacccggc catgtttttg tgccgttaaa catcatatgt tctacaaaat gagctacgcc
     2581 gcgctcattg tcttcttcct gcaatgaacc ggtatgaatt tgcagccata aatttacctg
     2641 atcctttgga tgagcatgcg gataaatcat atatcgcaag ccattgccca gttgcccggt
     2701 aattaacttt tcatcctgcg gtaaggcggc ggcaatcagc cgcccgggga gcaacagagt
     2761 tgccactaac gtcagtaaga aacagaggtt tctcataatt atctccatgc gaaaaccggg
     2821 cgaatttacc cggttaagta aaatccgaac tattaaaatt tcatgctgac atcaagccag
     2881 aaagtacggc cactggcgta ggtcgccaca ccggtattcg ttgtatcaac agcggttttg
     2941 ctatccaata cattgagaat atcggcactg atcgtcaggt tttgttgttt cagaaattgt
     3001 ggcgtccagg acaagcgggt gtcccaggtc aggctgctat ccagtttttc gtcaacatac
     3061 tgcttgtaat ctgaatattc gctgatgtat tgcgcatttg tcttacccag gataatgcga
     3121 gctttacgcg cttcttgcca ggccagcgta ttcgcccaca ctaacccgct cggttgatgc
     3181 gtgaaatcca tgtttaagga gatctttaat gggttattaa aatctgccac tggaacgcta
     3241 tcgtaagaga ccagattacc gttataaacc acctggttat caccggtatt gctctcctca
     3301 taaccattgt ttaacgacaa gttgcccttg ctcttgatat agctaaagac aatttgtggg
     3361 ttaatatcta cctggcggat atgcaggggt tcggccagtt caaaactgag gctgaacgaa
     3421 tgcgttttgg ttttgccatc gttgttatat tcagtaatgg tggttttagt cgcgctgtcg
     3481 gtacgactgc ttttgcttat ttgatcatgc gcttcacggt aaacatagtt tgcacgcgca
     3541 ataacgttct taccgatttt ctgctgcaat cccattgcca gttcatcgtt ataaggcgtt
     3601 ttcaaatcct gataacgcgt cagagtttta ttacctgata ccgattccgt ccagctattg
     3661 cggatatcac gtaatcccat atcaagaata ttcccgccat agtaacggtt ataacctgct
     3721 gtaatcattg aggtttgatt agcaaaaata tcccattccg tcataaagcg cggggagata
     3781 ttgtggtttg acagatagtt gtcatagtca taccgcacgc cgggcattaa tgacacatta
     3841 cgccagctaa tgcgatccgc catatacagc gtgtagttgt caattcccag gctgccttta
     3901 cctttatggt aaatggtatg gttagttttc tttccggcag cattaatcac ataggattca
     3961 gactggttat ggcgttcagt ccacgcatcg gaatagatgt attccgcgcc gaagtagggt
     4021 tgatgcgaaa cattacccac ggcgaatttt tgccagtcca ggcgtgtttt gaaggtgtaa
     4081 ttatctacag cctgggaaat gtgtcctaat ccgccacggg tgcaacgccc tgtaatatca
     4141 ccatatgtac atgaaagttc ggtgtaccag atgtcatgat cgtgacgggt ataatcacta
     4201 atatgatccc aaccaacggt ggtacgtagt ttggcccatg cgagctgcgt atccatatcc
     4261 catgccagac cataggattt attacccatt tcgcgatcag actgcgggaa ggtgctggta
     4321 ttataatcac ggctggagcc ggtatatttt aaggttaaat cgtgggtaaa gcggtcgctg
     4381 gcaaaccagg taaatttgct caatgcggta tcgataacgt ttttatactg tgcccgaccg
     4441 gcgacaatgc cgtcattcga aacataatcc gcgcgggtga tatcagactg gcggcgcgat
     4501 aaaccggcgg taacgccaaa gttatcagcg agttcctgat taaacgacaa ggtataaaag
     4561 ttctttttaa aatctgggga gtaataagta cttcctgaag agccttgatt aaatgcgctc
     4621 ttgttattct catcgatatg cgatgttaac cagtccgaac gcgtagtgcg ataacccagt
     4681 ttcacgctgc tatcatcagc gttgaagcgt ttgatctttg catcaattac cccgccattg
     4741 aagcgaccga attcaaccgg cacaaaactg tcataaagcg tcacattgtc cagtaagctg
     4801 acatcaagat aatacccctg tgacatcccg ctaatattgg ttgcactact ggcatcggac
     4861 tcattcgctg ggttcaagtt attagtggca ctaataccgt caatcaaata ggcattctga
     4921 tagggcgacg caccgtgaat agagattttc tccgggcgaa tatctccctg gttcaacgag
     4981 gtactttgcg ttgaatccat gcgtaccgca gggttggttc tcagcagatc gctgatattg
     5041 ccattcccgg tcggtaaacg ctcgatgctt tcactggtgt aatgcgtgtt gccgtttacg
     5101 ggtacaggga caggagcata gaccgtcatc tcttcaaaaa catgcatgta catgtttttg
     5161 tcatcgacgg cctgcgccac atcagcgcca cataaaatga cgccaggaat aagaactcgc
     5221 ttcatttatt tatatctccg tacagcaggg cttttattgt ttttataaaa ccgcgctaat
     5281 gtcacaaata tcatctgcca ggttccagac accgggttga tgtgtaacca taatgacgcc
     5341 gcttgtgggt agtttttcac gcactaaacg cagtaagcgg atagcctctt gttcctcaag
     5401 atgagaggta gtttcgtcaa gaaatatcca tttcggacgt cgtaaaatta atcgtgccag
     5461 ggcgatacgt tgtttttcgc cgctggaaag aatatctccc cagcgatcgt ggtcatgaat
     5521 acgcgcagcc aatttcccca gaccaacctg atgcagtact tcgctcaacg atttatcgtc
     5581 tacgggcagg ggaagtgctt tacaaataat ctctttcagt aagccgcttt tgattaacgg
     5641 tgtttgtgac acataccaac tgtcagcagg agaagaaata tcacctttaa accacggcca
     5701 gcagtgggat aatgttttaa gcagtgtggt ttttcccgca ccagagtagc ctttcagtaa
     5761 tagccatttg cctggcgaaa catgaaagtt caagttctct aatatgatct tattatcagg
     5821 cgtacgaata ctcgcattag ccacttgtac cgcatgttgg caatttttag gcttattcgt
     5881 agggcgctgt tcagtgagtt gatggaactc atacaagcga tcgataaccg cagccagctc
     5941 agcaagttcg tcatatttat aaataaacca gcttaaattg ttcgatacca gcataaatgc
     6001 ctggcgcgat ttcatcagtc cgcccagatt gatctgcccg ctaataaact gcggcaataa
     6061 cagaaagtag ggaagaacgc taagcgagcg cgaataaata ttctgccagt aatcaagcca
     6121 ccgctgacga ttcattaaac gatgccagtt ctctttaatg gtatgaaaat tgtcgctcaa
     6181 ctcctggcgt tgtagagact cagcattgct tagcgcaatc agttctgcct gcttattatg
     6241 ctgcacaaga tttgttcgaa atgtcgcttc gctacgttgt ttttccacat taagcggacg
     6301 aatacgttta ccaaccttat gggtaaataa agttccaccg atcacaatga gcacaacggt
     6361 atagaccata tacccctgga tattccattc tgttccacca acagtgaatg agagcgtacc
     6421 cgcgctttgc caaagaataa cggtaaaggt gatcagcatg ctaagtgact ggatgaagcc
     6481 aaatgaaagg ctgagcgttt tgctgatcag taagagaatg tcttcagcga tacgttggtc
     6541 agggttatct gtatttttat gctcgccgta gatctgcgtg aagtagtagt ttttatctgc
     6601 aaaccaccga ttaaggtagt aatctgttaa ccattcgcgc cagcgaatag tcagtagttt
     6661 aattaaccat gttttgttta cggatattaa tacaaagatc cccagcaggg cagggaacca
     6721 aaggacaagc tgccaaagct tatcggtttc tttctggctt agcgcattga aaaaatcatt
     6781 attccagtcg tttaaccaga cctgaatttt aaccacgcca aggatcatgg cgaggataat
     6841 aatgattaac aacactgaag ttttattatt cttacgcagc caaaagggct tcagcagaca
     6901 gagatacttt gctatcaaca tacaaaacgt aatgggaatg gttatcatta gcgaaaattg
     6961 attgtgccat ccaatgatta tctatggcaa tgatttactg atgaa
//
LOCUS       DAZFWP020000213         6985 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFWP020000213 DAZFWP020000000
VERSION     DAZFWP020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.214"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..6402
                     /gene="efa1"
                     /locus_tag="ACHK0Z_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             <1..6402
                     /gene="efa1"
                     /locus_tag="ACHK0Z_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HGU4504466.1"
                     /translation="EQKLQLIKALKEISGNTEKDSHYDRLLDAFFKKHNENIHNKIQR
                     IKDEFKEYSRVAIHNIDKVIFKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITGVHTE
                     ENLLPAPSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELPPH
                     ELLTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVR
                     MREGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIA
                     DATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPV
                     ASSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMG
                     LALLYLQTKDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQY
                     QGNKYITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPN
                     FGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPE
                     NDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIP
                     SLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQ
                     TRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDG
                     ETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDS
                     TLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGG
                     TMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLG
                     LTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVV
                     ASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVG
                     YANSISPYSSLAHGYANSKWPRTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVW
                     REAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYK
                     FILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLTFDLSKQTQSVMLK
                     TPDDEVPVMTITQKGINTLVGTAAGKDRLIGNDKDNTFHTSSGGGTVISGGGNNRYII
                     PRDLKTPLTLTLSSNSVSHEIFLPETTLAELKPVAFELSLIYWAGNNINVQPEDEAKL
                     NHFAGNFRVHTRDGMTLEAVSRENGIQLAISLCDVQRWQAVYPEENNRPDAILDRLHD
                     MGWSLTPEVRFQGGETQVSYDPLTRQLVYQLQARYSEFQLAGSRHHTTAVTGTPGSRY
                     IIMKPVTTQILPTQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQ
                     LTISGIEESLPETTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLM
                     ALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNIS
                     DLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDN
                     EAPSLILSEFRRFTISSDKTFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLT
                     FRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAE
                     YARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGELFPTFHPWYTDDL
                     SGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSI
                     LSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDS
                     HSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYI
                     RSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGAD
                     IADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLF
                     N"
     gene            6574..6924
                     /locus_tag="ACHK0Z_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /pseudo
     CDS             6574..6924
                     /locus_tag="ACHK0Z_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2117 a         1562 c         1564 g         1742 t
ORIGIN      
        1 gaacaaaaat tacagcttat taaagcattg aaagaaattt caggaaatac agagaaggac
       61 tctcattacg atagacttct tgatgctttt tttaaaaaac ataatgaaaa tattcataat
      121 aaaatacaaa gaataaaaga cgagttcaag gaatactccc gtgtagccat tcataatata
      181 gataaagtta tatttaaagg gcaaacactg gatcgtcttt atcatgaagg atatgtattt
      241 tctgatatca ataccttgtc tcgttataca ctacacggac taggaataac tggtgtacat
      301 actgaagaaa acctgctacc agctccttca tcgtccttaa ttaatatatt gaaagaacat
      361 tataatgaag atgaaattag tgcgaaatta ccactagcat atgattacat tttaaataaa
      421 aaagaatcaa gctctattcc tgttgaaatt ttgaacaaac tttcagagtt accaccacat
      481 gaactactca cacctgttct tggccagagt gttaatcctc tgggcatggg ctactcatcc
      541 gataatggaa aaatcacaga gcaagtaata gtcagtggag ctgatggatt tgataatccc
      601 atatctggac ttatatatac ctatcttgaa gatctatata acatccatgt aaggatgcga
      661 gaaggtacac taaattcaca gaatcttcgt cagcttctgg aaaactctgt ttcttcatgc
      721 tttttgactg agcaaagtat taataaatta cttagtgagg cagaaaaaag gccttatcag
      781 tctttaacag aaatacatca gcatctcaca ggattaccaa ctattgccga tgcaaccctt
      841 tcattacttt ctgttggatt acctggtacg ggaaaactat tgcgcaggga gcaggactat
      901 gggcgtccac cagttacagc aattcaggat tccacatttg tactccctta taatttcaaa
      961 ggtattggtt ttaacgataa cattatatcc tctgcacctg tagcctcctc attacatttt
     1021 atcgctgaac atgcgaaata tactttattg tcatggcctg agttttatcg tcatcatgca
     1081 cagcgatggt tcgaaatggc taaaggatat ggaagccaga atattgattt tcaccctcag
     1141 tctctattgg taacccaaga aggacgctgt atgggattag ccttacttta tttacagact
     1201 aaagatactg ctcattatag cattctccag gaaaacctaa tgactgtgag tgcacttcat
     1261 cagaccagta atcgcgataa gttgccactg tccaaagatg ataattcctt aatgacaaga
     1321 acttatagtc tgattgaaat gctacagtat cagggaaaca aatatattac caacgaatcg
     1381 ctactacata agaccgcatg gaaccaagaa agaataactt tattattcaa tgaaaaagga
     1441 gttaagcgag ccctgataag cacgcctaat catactctgg ttctgcaaca actggaggat
     1501 atttaccggc tcactgatcc aaattttggg catgcagatt tcctttcacc tatagatgct
     1561 ctgaagttta ttgaggctat gatacaatta actccaacac ttcaggaata ttatggccta
     1621 ttaaacaaag acattaataa acatatacaa gtacattatg ccgaatcaga tatggtctgg
     1681 aataagcttc tgccagaaaa tgatgctgga ctgagcacca gaattcagca caccaccacc
     1741 gaccgtctgg cgaatctggc tgaaccagtc gctgttgcag gtatctccct gccagtaaaa
     1801 acactttatg atatcggagc cacccttgac ggtcggcgca tcacctctcc tccaacatcg
     1861 gagcaaatcc cttctctgcg tctcaacggt gatgttctga atgattatct gtcccgcaca
     1921 gttctgactc cagaacaggc tgataacata agaaaaatac tgcacactca gggaatacgc
     1981 agcggtaccc gtcccataga tccggagatg attcgtggga cgcaggatga cctagtttcg
     2041 tcacagactc gtctgcaaag gcaagcaaca cgggttaaac agcaactcgc cggtgtactt
     2101 gatactctgc aacagcactt ccagaacatt ccacgttcat ccggtcgtca tctttctgta
     2161 gagaatattg agctggctga tatcggaagt gggcgtttca accttcaaat tcgagatgga
     2221 gaaacattgc ataccacttc tgtggaagta ccggaagttg tgtcccgttt tcagaaactt
     2281 tctaccatgc tttcagccct gcctgccagt ggaatcatgg atttcgacct cggcatgagt
     2341 gtggtcggag tcgtccagta tgcccgcctg ctacagcagg ggcacgaaga cagtacccta
     2401 gccaagataa atctagctat ggatatcaag caactttccg aagcaactct cggcagcatg
     2461 attcagattg ccgggaataa gtttctcaat acagaaggaa tccaggggtt cagactggaa
     2521 agcgccgtcg ctgaaggtat gcgctcagta gcaacccgta ccggaggcac aatggggaaa
     2581 gccctttctg ccagtgcccg tgttcttgaa ctgcctgtac tggaaacagt tctggggaca
     2641 tggaacctgt acaacagcgt cattcagctc cagcaagcta catcttattc tgagacaatg
     2701 gctgcccggg tacagattgc gtttgattcc atttctctgg gattaactgc cgcttcggta
     2761 gctttcccgc cactaattat tgccactggc cccattgcgg ctattggtat gggagcttcc
     2821 agtattgcac gtaatgtggc acggaaagaa gaacggcata cacaatggct ggaatataaa
     2881 aaattcctga ctgatggcag taaacacatt gttgtggcct ctccggaaag aggtctgctg
     2941 gatttctccg gaaacaaagt ttttggaaaa atggtgctgg atctgcgtca gtctcctcct
     3001 ctcttgcatg gagaaagctc ttttaacgct gaccgcaaaa tcggtcatcg tccggatctg
     3061 ggggactggc aaattcgtga gaaggtgggg tatgccaaca gtatcagtcc ctactcttct
     3121 ctggcgcacg gttacgccaa cagtaaatgg ccacgaacaa taccgaaaat tccctcggga
     3181 gaatatgaca ccataattct gggctacggt caccagtatc aggccaatac ggaaatagaa
     3241 tatctgtcta actggattgt atggcgggaa gccgtaccag acagtacttc ccgccacaaa
     3301 cgtcctcctc tggaggttct taatagtcag tgtactgtga tagctggaga gcgtaaaacc
     3361 acagtacttc ccctgagagt gctcagcgat ctgacaccgg aatgcacaga acaggctata
     3421 tcgttaaaag attataaatt catactgaga gggggaagcg gtgggctggc tgttcaggtc
     3481 ggtggcgcgg gatattatga tattgatgca aatcctgtgg caaaagaaaa tacgctctct
     3541 tttcgcgggc taccggaaga gtttccgctc acctttgatt tatcaaaaca aacacagtcg
     3601 gtcatgctga aaacaccaga cgatgaggtg ccggtaatga ccattaccca gaagggaata
     3661 aacaccctgg taggtacagc cgccggtaaa gaccgactaa tcggtaacga taaggacaat
     3721 accttccata caagctctgg cggcggtaca gtcatctccg gaggcgggaa taaccgctat
     3781 attatccccc gggatttaaa aacgccgttg acactgacgc tgtccagtaa ctcagtctct
     3841 cacgaaatct ttctgccaga aacaacccta gctgaattaa aacctgtcgc ctttgagctg
     3901 agtttgattt actgggccgg gaacaacata aatgttcaac cagaggatga agcaaaactg
     3961 aaccactttg ccggaaactt cagggtgcat acccgtgatg gcatgactct ggaggcggtt
     4021 tcccgggaaa atggtattca actggcgatt tcattatgtg atgttcaacg ctggcaggct
     4081 gtttatccgg aagaaaataa cagaccggat gccatactgg acaggctgca tgatatgggc
     4141 tggagtctga caccagaagt ccggttccaa ggaggagaaa cacaagtcag ctatgatccc
     4201 ctgactcgtc agctcgttta ccagcttcag gcgcgttact ctgaattcca gttggccggt
     4261 agtcgccacc ataccacggc tgtaaccgga actccgggaa gccgatacat tatcatgaag
     4321 ccagttacaa cacagatatt accgacacaa atcatactgg ctggtgataa tgaccatccg
     4381 gaaacgattg atttactgga agctagtcct gttctggttg aagggaaaaa agacaaaaac
     4441 agcgtgatat taacgattgc tacgattcag tattcccttc aactgacaat atccgggatc
     4501 gaagaatcgc tgcccgagac aacccgtgtg gcaattcagc ctcaggatac ccgtttactg
     4561 ggtgacgtac tccggatctt accagataat ggtaactggg tggggatttt ccggagtggt
     4621 catacaccaa cggtaaaccg gctggaaaat ttgatggcac tgaatcaggt aatgacgttc
     4681 ctgccccggg tatccggaag tgcagagcag gtattatgcc tcgaaaacct aggtggcgta
     4741 aggaaaaaag tggaggggga gttactgtca gggaagctga aaggtgcgtg gaaagccgaa
     4801 ggtgaaccta ctgttccggt aaatatctca gatctaagta tcccacccta ttcacgtctg
     4861 tatctgattt ttgaagggaa aaataatgtg ttgctacgca gtaaagtaca tgcagctccg
     4921 ttgaaaataa catccgcagg agagatgcag ttatctgaaa ggcagtggca acagcaggaa
     4981 catattattg tcaagcccga caacgaagcc ccctcattaa tactcagtga atttcgtcgt
     5041 ttcactattt catcggataa aacattttct ttaaaactga tgtgccatca gggtatggtc
     5101 cgcatcgacc gcagatcatt atcggtcaga ttgttctatc tgcgtgaaca gccaggtatc
     5161 ggcagtttac gtctgacgtt cagagatttt ttcacagaag tgatggatac aacggacagg
     5221 gaaattctgg agaaagagct gagaccaatt ctgataggag atacacaccg ctttatcaac
     5281 gctgcataca aaaatcatct gaatatccag ttaggagatg gcgttctgaa tctggcagac
     5341 attgttgcgg aatacgcccg tattcaaaag gaagaaacat caaaaatatt gtatcaatat
     5401 caaggtgcca tgaaaaaaaa aacagatgga ccatctgtgg tagaagatgc cattatgacc
     5461 actactgtca caacagattc aggtgaacta ttccctacct tccacccgtg gtatacagat
     5521 gatttatcag ggcgttataa gagcgtacct atggcaagaa aagcagatac tttgtatcac
     5581 ctgacaccaa aaggtgatct acagataata tatcaggtag ctacaaaaat ggtgaatcag
     5641 gcgatgattg tatccctgcc aaactaccga cacgagtggg aaaaatataa tttaagcatc
     5701 ttatccgaaa tccctcagaa caataatact gttgtacatt caatcctcag ggttaatggc
     5761 cccacaatgc aggtgcgcac aattgactac agaggaacgg atgaaaacaa tcccatagta
     5821 tctttttcag atacaacctt catcaatggt gaacagatgt tgagttatga ctcgcattca
     5881 tcagggcgag tctattccag agaagaatat atgatgtggg aattgcagca acgggtatca
     5941 gaagcttcca gtgcccggac acaggattac tggctgatgg atgcagcggt aagaaacgga
     6001 gaatggaaga tcacaccaga attattacgt cacacaccgg gatatatccg gagtacggta
     6061 tcgaaatggt ccagaggatg gctgaaaacc ggcacaatac tccagactcc agaagacaga
     6121 aatacggatg tatacctgac taccatacag aacaatgtat ttagtcgtca ggggggcggc
     6181 taccaagtgt attatcggat tgatggaatg gctggtgcgg atatagcgga taatgcacca
     6241 ggggaaaccc gctgcaccct caggcccgga acatgttttg aagtgacaag tgtggatgaa
     6301 aggcattatg agtggaatat catttatgtc acgctgaaaa cctgtggctg gagccgaaat
     6361 ggccaaagca aaacgccgaa tggtgacaac ctttttaact aattaaaatc tctgttaaag
     6421 atgtataacc cggcatctat gtgctcacca gcatatagat gcaatgtgat tcccctgatg
     6481 gtcgtatcag agatggtatg cgagcctgta catagatttg tgtaattgcc tgattttgat
     6541 atgttcaatc caacatcaaa agcaggttaa tttatggacg aaaaacagtt gcaggctctg
     6601 gctaacgaac tggccaaaaa tctcaaaacc cctgaagatc tcagccagtt cgatcgcctg
     6661 ctgaggaaaa tcagcgttga ggcagctctc aacgctgaaa tgtcccacca tctgggctac
     6721 gataaaaacc agcctaaacc gggtgccaac tcccgcaatg gctattccac aaagaccgtt
     6781 atcatcggtg atggccccct ggaactacgc actccgcgcg atcgtgatgg ctcttttgaa
     6841 ccgcaacttg tgaagaaaaa ccagacccgg atcaccggga tggataacca gatcctgtcg
     6901 ttgtacgcca aaggcctgac aaccgtaagc gtacagcctg aaccgtctgg tcagaatctg
     6961 acgaattaga gtaagcgtaa accga
//
LOCUS       DAZFWP020000214         6958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFWP020000214 DAZFWP020000000
VERSION     DAZFWP020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.215"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="ACHK0Z_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="ACHK0Z_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU4504467.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMEVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MEITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="ACHK0Z_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="ACHK0Z_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU4504468.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..4014
                     /gene="araH"
                     /locus_tag="ACHK0Z_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             3028..4014
                     /gene="araH"
                     /locus_tag="ACHK0Z_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU4504469.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4181..4981
                     /gene="otsB"
                     /locus_tag="ACHK0Z_RS22920"
                     /old_locus_tag="pgaptmp_004584"
     CDS             4181..4981
                     /gene="otsB"
                     /locus_tag="ACHK0Z_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU4504470.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4956..6380
                     /gene="otsA"
                     /locus_tag="ACHK0Z_RS22925"
                     /old_locus_tag="pgaptmp_004585"
     CDS             4956..6380
                     /gene="otsA"
                     /locus_tag="ACHK0Z_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU4504471.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHK0Z_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHK0Z_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU4504472.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1761 a         1630 c         1811 g         1756 t
ORIGIN      
        1 taatgagcac agtgataatt aatggcgaaa tgaatgacgt gcattttcca catctttgag
       61 ttgcggttat tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt
      121 aaaaagaaga catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg
      181 gattaatctg ctgtgcattc gacaatttgt ctgacaaatt ggctttccct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacagtcact tatcttttag ttaaaaggta
      361 atgctttgtt ttccgattaa tttaacgaat gtcattcgtt tttgccctac acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggttttc tggtgaagca
      541 accggaagag ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgag gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga
      661 cagcctggct gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg
      721 ctctgccatc gtcgcgaaag cgcgtggcta cgatatggaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac
      841 taaaattggc gaacgtcagg gccaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggagattac cgccaacgaa ctggataccg cccgccgccg
      961 tactacggga tctatggatg cgctgaaagc ggccggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg gcattggcat
     1201 taacggtgtg gatgcggtga gcgaactgtc taaagcacag gcaaccggct tctacggttc
     1261 cctgctgcca agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt
     1321 agcaaaagac gttgaaccgc caaaatttac cgaagttacc gacgtggtac tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa
     1741 cgcgggtgtg gcgattattt accaggaact acatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa
     1861 ttatgaggcg ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaggcgc tggcgcgtaa
     1981 cgctaaaatt atcgcctttg atgagccaac cagctccctt tctgcccgcg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccatcact gtctttaaag atggacgtta
     2161 tgtcaaaacc tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt
     2221 cgggcgcgac attggcgata tctacggctg gcaacctcgt agttatggcg aggagcgcct
     2281 gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggtgaaatt gttgggctgt ttggtctggt aggggcgggg cgtagcgaat taatgaaagg
     2401 cttgtttggc gggacgcaaa tcaccgcagg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcccg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 taaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatca tgaatctctc
     2701 aggcggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcggcattga tgttggtgct aagcatgaaa tttacaacgt
     2821 gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc ggattgtggt gatgcgggaa ggtgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg gtctggcgca
     3061 cctaagtcgt cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtgctct ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg
     3181 aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc
     3241 cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc
     3301 acggcggtgg ttatcaacct gactgaaagc ctgtggattg gcgtggcagc ggggttgctg
     3361 cttggcattc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct
     3421 ctgatcacaa cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc taactggttc
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgttgctg
     3601 aataaaacca cctttggtcg taacaccctg gcgattggcg ggaacgaaga ggctgcgcgt
     3661 ctggcgggtg taccggttgt tcgcaccaaa attattatct ttgttctctc tggcctggta
     3721 tctgcgatag ccggaattat tctggcttca cgtatgacta gtgggcagcc aatgacgtcg
     3781 attggttatg agcttattgt tatctccgcc tgcgttttag gtggcgtttc tctgaaaggt
     3841 ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac
     3901 gccatgaacc tgcttaatat ttctcctttc gcgcagtacg tggttcgcgg cttaatcctg
     3961 ctggcagcgg tgatcttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt
     4021 tttccacaac aatttaacgt tttttcccac cacagccagc cgccacaacg gttggctgtt
     4081 cttcattgca aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt
     4141 tctgcgcaaa acaataagaa aagagaagga ggagagccgg gtgacagaac cgttaaccga
     4201 aacccctgaa ctatccgcga aatatgcctg gttttttgat cttgatggaa cgctggcgga
     4261 aatcaaaccg catcccgatc aggtcgtcgt gcctgacaat attctgcaag gactacagct
     4321 actggcaacc gcaagtgatg gtgcattggc attgatatca gggcgctcaa tggtggagct
     4381 tgacgcactg gcaaaacctt atcgcttccc gttagcgggc gtgcatgggg cggagcgccg
     4441 tgacatcaat ggtaaaacac atatcgttca tctgccggat gcgattgcgc gtgatattag
     4501 cgtgcaactg catacagtca tcgctcagta tcccggcgcg gagctggagg cgaaagggat
     4561 ggcttttgcg ctgcattatc gtcaggctcc gcagcatgaa gacgcattaa tgacattagc
     4621 gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt gtgttgtcga
     4681 gatcaaaccg agaggtacca gtaaaggtga ggcaattgca gcttttatgc aggaagctcc
     4741 ctttatcggg cgaacgcccg tatttctggg cgatgattta accgatgaat ctggcttcgc
     4801 agtcgttaac cgactgggcg gaatgtcagt aaaaattggc acaggtgcaa ctcaggcatc
     4861 atggcgactg gcgggtgtgc cggatgtctg gagctggctt gaaatgataa ccaccgcatt
     4921 acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta
     4981 accggattgc accaccagac gagcacgccg ccagtgccgg tggccttgcc gttggcatac
     5041 tgggggcact gaaagccgca ggcggactgt ggtttggctg gagtggtgaa acagggaatg
     5101 aggatcagcc gctaaaaaag gtgaaaaaag gtaacattac gtgggcctct tttaacctca
     5161 gcgaacagga ccttgacgaa tactacaacc aattctccaa tgccgttctc tggcccgctt
     5221 ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc ctgggacggc tatctacgcg
     5281 taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga
     5341 tccacgatta tcacctgttg ccatttgcgc atgaattacg caaacgggga gtgaataatc
     5401 gcattggttt ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga
     5461 catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacagaaa
     5521 acgatcgtct ggcgttcctg gattgtcttt ctaacctgac ccgcgtcacg acacgtagcg
     5581 caaaaagcca tacagcctgg ggcaaagcat ttcgaacaga agtctacccg atcggcattg
     5641 aaccgaaaga aatagccaaa caggctgccg ggccactgcc gccaaaactg gcgcaactta
     5701 aagcggaact gaaaaacgta caaaatatct tttctgtcga acggctggat tattccaaag
     5761 gtttgccaga gcgttttctc gcctatgaag cgttgctgga aaaatatccg cagcatcatg
     5821 gtaaaattcg ttatacccag attgcaccaa cgtcgcgtgg tgatgtgcaa gcctatcagg
     5881 atattcgtca tcagctcgaa aatgaagctg gacgaattaa tggtaaatac gggcaattag
     5941 gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat
     6001 tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ctggtagcaa
     6061 aagagtatgt tgctgctcag gacccagcca acccgggcgt tcttgttctt tcgcaatttg
     6121 cgggagcggc aaacgagtta acgtcggcgt taattgttaa cccctacgat cgtgacgaag
     6181 ttgcagctgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg
     6241 cagaaatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta
     6301 gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat aaagttgcta
     6361 cctttccaaa gcttgcgtag gagcggttaa tctcccgtaa gtggaactaa cagcacatcg
     6421 acctcacttg aggcaataac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga
     6481 ttgtgattac cgcaaatcac caaatcgaaa tgatgtttgt gacatacttc cagaatatgt
     6541 tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg gataccccgc atcctgaatt
     6601 aacttatcaa gaaagctttg cgtttcttct tgcatcacgc tgcgtaaatc ttcaagcatc
     6661 ggcgcggcta attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt
     6721 ccctttactg gcctggcgat agataccgct tttgccagca gttgctgact ttccggtgta
     6781 accgcaacag cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt
     6841 caggtgacat taaagcttta ctcctaatgg agaaacgacg caatcccaac tcggtcaaac
     6901 aggtaaatat tagtctgatt attagtaaag acaattaatc aatcactccc ggtgatca
//
LOCUS       DAZFWP020000215         6886 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFWP020000215 DAZFWP020000000
VERSION     DAZFWP020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.216"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(274..759)
                     /gene="sixA"
                     /locus_tag="ACHK0Z_RS22935"
                     /old_locus_tag="pgaptmp_004587"
     CDS             complement(274..759)
                     /gene="sixA"
                     /locus_tag="ACHK0Z_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU4504473.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(962..3106)
                     /gene="fadJ"
                     /locus_tag="ACHK0Z_RS22940"
                     /old_locus_tag="pgaptmp_004588"
     CDS             complement(962..3106)
                     /gene="fadJ"
                     /locus_tag="ACHK0Z_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU4504474.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3106..4416)
                     /gene="fadI"
                     /locus_tag="ACHK0Z_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             complement(3106..4416)
                     /gene="fadI"
                     /locus_tag="ACHK0Z_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU4504475.1"
                     /translation="MGQVLPLVIRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4596..4880)
                     /locus_tag="ACHK0Z_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             complement(4596..4880)
                     /locus_tag="ACHK0Z_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU4504476.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5252..6592
                     /gene="fadL"
                     /locus_tag="ACHK0Z_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             5252..6592
                     /gene="fadL"
                     /locus_tag="ACHK0Z_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU4504477.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1721 a         1853 c         1624 g         1688 t
ORIGIN      
        1 ttcaattaca tcctgcctga ttattcaact ctattttctc tattatctgt attaataaat
       61 gacaatgagt gtcaaacaga cattattcat ttcaacatgt aactttgggc aaaatcaaga
      121 tacgcgcaga agaataattt ccgaatgaat gtagaaagtg ttttatttat agataaggag
      181 agtcttcgat agtagtgagg ttgacagata tccgctgcct gatgagcaat tatgcccaat
      241 gaatctacct cattgggcat aatttggaac acatcagata gcttttgcca tcttcagatt
      301 acacgggctc atctgccagt tgaatgtacc gtttccgctt tcatcaagcg ttacgctggc
      361 aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcgg caaccagata
      421 cccgactaac ggtaagtgag agatcaccag taccgaagca acaccttcat tggtcaacgc
      481 ctgcaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg gcagaacttc
      541 tgcactggaa ggcaggttca gacaatcccc tacttcttcc agtgtttgct cggctcgcag
      601 gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc agttcgccat
      661 caggcgagat tcgtcacaac cattagtggt cagaggacga acggaatcac tggcggcatc
      721 gagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct tttgttaacc
      781 agattcgccc gttttctttc atctgaaacc agagaaagaa aacggtggcc ggcattgtgc
      841 cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt atagtcaata
      901 tgcgtttaca ttttgaccaa cactccgcca ttcagcgcgg attcatatag ctttgacctt
      961 cttattgcag gtcagttgca gttgttttcc aaaaactttc cccacgcgcg cccatctcga
     1021 ccaaacgctc gcaaggggta aaacgggaac catactgcgt ggcaagtcgt tgcattattg
     1081 caaccacttc gcccgcgccg agagaatcga tatagcggaa cggtccaccg agaaatggcg
     1141 gaaaaccaat gccaaatacc gcaccaatat ccccgtcacg cacgctacgg ataacctgct
     1201 catcaacaca acgtactgct tcattcagca tcaacatcac acaccgttca gcaacctgcg
     1261 gtgcggagag tcgcccctgc ccttgtgcgc caatgagcgg gtaaatggcg ggatcgacct
     1321 gttttttgct tttacgccct ttctgaccat aaagatagaa accccgacca ttttttctgc
     1381 ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta aaacgttctc
     1441 cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct acctcatcca
     1501 aaagttggat tggacctacc ggaaaaccaa atttcactag cgcggcatca atgtgctcta
     1561 cccgttcacc ttcggtcagc atgcggatag cttcattaat gtaaggcgct aagatgcgat
     1621 tgacgtaaaa cccggctttg tcacgcacga caatgggcgt tttgccctgt tttttcgcca
     1681 gttttaccgt agtggcgatg gtttgcgctg atgtacccgc atgaggaata atctccacca
     1741 gcggcatttt ttccaccgga ctgaagaaat gcaggccgat aacttgctca ggtcgcgtgg
     1801 cgtgagcggc gatatcacca atcggtaaag atgacgtatt cgaagcaaag atggtatgag
     1861 cggcgctatt ttgctcaact tccgccacca tctgttgttt caattcgaga ttttcaaaca
     1921 ccgcttcaat aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt ccggagatta
     1981 atgccagctg tttgtcacgt tcgctggctt tgagatgacg acggcgaact ttgccctcca
     2041 gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata tctttaattc
     2101 tgaccggaag ccccgcttta caagcagtga cataagcaat accgccgccc atcaagccac
     2161 cacctaaaat tcccacgctg tttaatggcg caggcggtgc atcactaccg ggatctttct
     2221 tcacgtccgt actggcaaaa aagatactac gcagcgcctg cgattgtggc gtcatcgcca
     2281 gttcgccaaa cgcccgagct tcggcgtcat aaccgctgct ggtgccctgc gctaatcccg
     2341 tttcaacaac ctccaggatg cgttctgtcg ccggataatt gccttgagtt ttgtgttctg
     2401 ttttcttgcc gaccattttg aacagcagcg cacgacctaa cggccccgcc agaatacgct
     2461 cgcgtacagg tagagggcgg gaagatgggc gatccttctt cgccagctca acagcgactt
     2521 ccagcaaaat ggagtgcgga acaacgtcat ccaccagccc cagctttaat gcctgtttcg
     2581 cccgaagttg ttttccggtg aggatcatct ctaatgctgt gctgacgcct atcagacgcg
     2641 gtaaacgctg ggtgccgcct gaaccgggta acaatccaag ttgtacttca ggcaaaccga
     2701 gcaccgtttt aggatcgtca gtacaaacgc gaccgtggca cgccagcgcc aactccagcc
     2761 caccacccag gcaagcgcca tgaatagccg cgataaccgg aatgggcaaa gcatgaatct
     2821 ccgccatcaa ctgttggccc tgccgcgcca gtgcttccgc ttcttgcgcc gttttgcagt
     2881 tgccgatcat gttgatgtct gcgccagcaa tgaagttgtc cggtttagcg gagacaaaca
     2941 ccacgcctcg caactctttg ttttcacgga gttgcttaat aatggcgcgc acctgcgagg
     3001 caaactccgc cttcagggta ttcattttct cacccggtac gtcgatggtg atgatagcaa
     3061 tgttgtccag acgaacatta agagtaaaca ctgatgccat ttccattatt ccgcctccag
     3121 aaccattgcc gcgccaagcc caccggcagc acaggcggta actaaaccaa atccaccgcc
     3181 gcgacggcga agttcatgca atgtctgggt aatcatccgc gcgccggtcg ccgcgaaggg
     3241 atgcccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca cttcgccagt
     3301 ggcatgtgca cgccccagta cttcacgagc aaaacgttca ctacccagca actgaatatt
     3361 cgccagtgtc tgagctgcaa aggcttcgtg catatcgatc aatgtcagat cgctcattgt
     3421 caaaccggca cgttccagcg ccagcggtgt tgaccaggcc ggaccgagca acatgtcctg
     3481 ccagacatca atcgcagtaa atgcgtagct gcgcagatac cccagcggca ccagccctaa
     3541 ttctttcgcc cgggattcag tcatcaggat caccgccgct gcgccatcgg tcagcggcgt
     3601 actgttcgcc gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca gctttgcgta
     3661 atcggcaagc gaggaattac cgcgaatatt gttgtcttcg acaagcggtt gtttataagg
     3721 agggataaag gcagtcatca cctcttcttt gagtttaccg tctgaccacg cctgagcggc
     3781 acgctgatgc gaacggtgcg ctaatgcatc ttgctgttct cgggtgatgc cgtaggtttt
     3841 cgccatttgt tcggcggtgt cgcccatccg caagccagta gaatattctg ctaccgcagg
     3901 cggtacgggc attaagtcgc gcaaacgcag gcgagagaag agtttcagtc gctggctcat
     3961 ggttcgagct ttgttgacat caaccagcac gcgcgccagt tttttactga cgccaattgg
     4021 caatacggaa gaggaatctg ccccaccggc aatccccgct cggatagttc ccgccatcag
     4081 gctttctgcg acgtttgcaa ccgcctggaa actggtagcg caagcgcggc tgacgctgta
     4141 agcatcggta tgcacattca ttccggtacc gagaacaatt tcacgcgcaa tgttgggggc
     4201 ttcaggcatt tgtacgacct gaccaaagac cagctgttca atcacttcag cggggatctc
     4261 gctgcgtgcc agcagttcgc ctaccaccat cttccctaaa tcaaccgcgg gaatgccatg
     4321 aaaagccgtc gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg cgatacgatc
     4381 gccctggcgg ataaccagcg gtaaaacctg acccataaca ctcccctgta aaaaaataaa
     4441 taaagtggtc tgacctgatc atagtcttaa ccattttttt acatttagcc aagcggagaa
     4501 aaaagaaagt gggagctatg acacagtgaa gacaggagaa agaaaagaaa acgcccctgc
     4561 cagcaactga caggggcgaa cgtacccgag aggaattaac gcagacccag ctggaagatc
     4621 agcatttcag cttcgcaggc aaaagtgaaa tcgatatcca ggcgcacacc gtcagactct
     4681 tcagtgaaag tcggggtgat tttgcacggt tcggattcca cgctacgggc tttttcagtc
     4741 agcgccgcca gcgtttgttc tgcttctgcg cggcttgcaa acacgcggct gtaagacgcg
     4801 gtgcagtcgg agttgtccat aatggtgcca acatccatac agcagcaaac cggggtttca
     4861 tcagcactac atttactcat cgttgatttc ctctgtatgt gcacccaagg tgccagataa
     4921 acgttgtgga tattttacgc ttccggaaag tgctgctcca gttgttaatt ctgcaaaatc
     4981 ggataagtga ccgaaatcac acttaaaaat gatctaaaac aaaattcacc cgaatccgcg
     5041 agtgcgcaac ctccaaattt tgccagctgg atcgcgtttc ttagatcata tttgaaaaaa
     5101 gatggaaaca tacttgcaac attccagctg gtccgaccta tactctcgcc actggtctga
     5161 tttctaagat gtacctcaga ccctacactt cgcgctcctg ttacagcacg taacatagtt
     5221 tgtataaaaa taaatcattg aggttatggt catgagccag aaaaccctgt ttacaaagtc
     5281 tgctctcgca gtcgcagtgg cacttatctc cacccaggcc tggtcggcag gctttcagtt
     5341 aaacgaattt tcttcctctg gcctgggccg ggcttattca ggggaaggcg caattgccga
     5401 tgatgcaggt aacgttagcc gtaaccccgc attgattacc atgtttgacc gcccgacatt
     5461 ttctgcgggt gcggtttata ttgacccgga tgtgaatatc agcggaacgt ctccgtctgg
     5521 tcgtagcctg aaagccgata acatcgcgcc tacggcatgg gttccgaaca tgcactttgt
     5581 tgcaccgatt aacgaccaat ttggttgggg cgcttctatt acctctaact atggtctggc
     5641 aacagagttt aatgatactt atgcaggcgg ctctgtcggg ggtacaactg accttgaaac
     5701 catgaacctg aacttaagcg gtgcgtatcg cttaaataat gcatggagct ttggtcttgg
     5761 tttcaacgcc gtctacgctc gcgcgaaaat tgaacgtttc gcaggcgatc tggggcagtt
     5821 ggttgctggt cagattatgc aatctcctgc cgggcaaact cctcaagggc aagcattggc
     5881 agctaccgcc aacggtatcg acagtaatac caaaatcgct catctgaacg gtaaccagtg
     5941 gggctttggc tggaacgccg gaatcctgta tgaactggat aaaaataacc gctatgcact
     6001 gacctaccgt tctgaagtga aaattgactt caaaggtaac tacagcagcg atcttaatcg
     6061 tgcgtttaat aactacggtt tgccaattcc taccgcgaca ggtggcgcaa cgcaatcggg
     6121 ttatctgacg ctgaacctgc ctgaaatgtg ggaagtgtca ggttataacc gtgttgatcc
     6181 acagtgggcg attcactata gcctggctta caccagctgg agtcagttcc agcagctgaa
     6241 agcgacctca accagtggcg acacgctgtt ccagaaacat gaaggcttta aagatgctta
     6301 ccgcatcgcg ttgggtacca cttattacta cgatgataac tggaccttcc gtaccggtat
     6361 cgcctttgat gacagcccag ttccggctca gaatcgttct atctccattc cggaccagga
     6421 ccgtttctgg ctgagtgcag gtacgactta cgcatttaat aaagatgctt cagtcgacgt
     6481 tggtgtttct tatatgcacg gtcagagcgt gaaaattaac gaaggcccat accagttcga
     6541 gtctgaaggt aaagcctggc tgttcggtac taactttaac tacgcgttct gataacgcgt
     6601 tcgcctggat aaagtcacct gcatagcagg tgactttaac tcccccactt caccgaaagt
     6661 agtgtcctca ttgcttacct cctgagtttt gcaaacagcc tgttggcagc ttgccctttc
     6721 aaatcagtaa gtggtattca cagacaaacc tgaagggaaa tgcatatttt caagcgttct
     6781 gctcgtcctt ctcaaagagt ttacttttct gaatttccag gatactcccc ccccccctgg
     6841 ctattgtgcg ctcatacact caaattaaag ataggttcta aataaa
//
LOCUS       DAZFWP020000216         6865 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFWP020000216 DAZFWP020000000
VERSION     DAZFWP020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.217"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            297..503
                     /locus_tag="ACHK0Z_RS22960"
                     /old_locus_tag="pgaptmp_004592"
     CDS             297..503
                     /locus_tag="ACHK0Z_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4504478.1"
                     /translation="MNTPVSLMDDQLVDMAFITQLTGLTDKWFYKLIRDGAFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            592..1194
                     /locus_tag="ACHK0Z_RS22965"
                     /old_locus_tag="pgaptmp_004593"
     CDS             592..1194
                     /locus_tag="ACHK0Z_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504479.1"
                     /translation="MKQQYQTRYESLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWISFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKHHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWTENLKRK
                     SEKELEEWIIDRQKQNAALTDLMDQYVLLAYRTTVDDNRN"
     gene            1484..2138
                     /locus_tag="ACHK0Z_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /pseudo
     CDS             1484..2138
                     /locus_tag="ACHK0Z_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2811..4319
                     /gene="ltrA"
                     /locus_tag="ACHK0Z_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             2811..4319
                     /gene="ltrA"
                     /locus_tag="ACHK0Z_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGU4504480.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRLIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTKH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTGTRNTYTSRFTEVAMAFASI"
     gene            4403..4882
                     /locus_tag="ACHK0Z_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /pseudo
     CDS             4403..4882
                     /locus_tag="ACHK0Z_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5923..6795
                     /locus_tag="ACHK0Z_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             5923..6795
                     /locus_tag="ACHK0Z_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504481.1"
                     /translation="MVKVVEDERSRIRYLERRLNENGFYLPSSLADKDYLSYQKRILN
                     TLISQGVDTLKINNFLAETDQRYFDSLPSENDLNWYRNDARASLWLTCELYEMIKING
                     YENTLTCLSPESLPSHHSVRVDAIRRCIDNWPFILYTPSNYLNQKSIEWTTLLEKDDI
                     FREVKARNVDICSWLKKYIQEKTNISLNYVCGESSEEIMAWCYASYFTWKKNNQNSPD
                     SVELFTRKFKSAWATQKNRIKNRVDKKLRPLNVNISQEAYDKLRKLSMNEGISNDRVI
                     ESALDMIYRSKIKK"
BASE COUNT         1905 a         1624 c         1755 g         1581 t
ORIGIN      
        1 ctaggaacag actgacttac tgtaaccgcc tcccaaaaca actctgcaat tatcagaacg
       61 ccggtttttc ccccatcctc acgaaaatca tcccttcttt atcgcgcgtg cgtgcagaag
      121 catcgtttcg cggtagccac cgggtatctc agcatcatgc tccctgttta tctcccggca
      181 ggaacattcc cctactcatt ttttacgttt ctccccggtt gttatgccgg tgcaggtgat
      241 gcgttgtttt catcacaaac gtcggttgag attaacaaca tccgaaggag attttcatga
      301 ataccccggt ttcgctgatg gatgaccagc tggtcgacat ggcatttatc actcaactga
      361 ccggcttaac cgataagtgg ttttataagc tcatcagaga tggtgccttt ccggccccta
      421 tcaaaatggg tcgcagctcc cgctggctga aaagcgaagt ggaagcctgg ctgcaggcac
      481 gcattgcaca gtcccgtccg tagttcataa ccgttatccg tttacctgca gcagacgcaa
      541 tttccggcct gtcggcggca ttctgctgcc tgttatatcc ccgagaggaa gatgaaacaa
      601 caatatcaga cccgctacga atcgctccac gaaagctacc agaaatggct gaccggcttc
      661 acccggcacg ctgtatcctg gggagtgtgt catccgaata tctactactt ccataatctg
      721 acgcccggat ggatatcatt caatggtgaa aaacccgaga tcgccatcgt accccagagt
      781 ctgcaccggc tgatttatgg ccctgacaaa cggaccacgc cgccgctgga tgatgatctg
      841 gtggtgaact tatgcaccag tgaacatctg ctggttcatc atccgatgct ggaaggcatt
      901 ctgctgtctg aatgtgaacg tctgaaacat cattctctgg caaataaact gatcagcctc
      961 ttccgtcagt ttggtggcac agagttgcgc ctcaaactgg tctggctttg ctggcttgat
     1021 ttaatgaccg gaaacagcct tgaagactgg acagagaacc tgaaacggaa atcagaaaaa
     1081 gagctggagg aatggatcat cgaccgtcag aagcagaacg cagcactgac ggatctgatg
     1141 gatcagtacg tgctcctggc ataccgcaca acggttgacg ataaccgtaa ctgatgccgg
     1201 ttgcttccgg aaggtgcggc agaatacggg aattatgctc ccggccccgc ctgctcacct
     1261 gcagagcgtg ttcgtcgtca tcatactgag ccatgatgaa tggcttaagg gcatcctgtc
     1321 attactgaac tggcatgaac accaccagtg gatgaaagcc acggacaggg atgttcttta
     1381 cagccagcta cacgccacgt tctctacgga ctgaatatgc aggcagaggt gaaccgcccc
     1441 ggaaatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatacacg
     1501 tttttccccc gaagtccgtc aacgggcagt tcgtatggtt ctggaaagtc agggcgaata
     1561 tgactcacaa tgggcggcaa tttgttccat taccccaaag attggctgta cgccggagac
     1621 tctgcgtgtc tgggtacgcc agcatgagcg ggataccgga ggcggtgatg gtgggctcac
     1681 caccactgaa cgtcagcctc tgaaagagct ggagcgtaaa aatcgtgaac tgcgccgcag
     1741 taacgatatc cttcgccaga cttccgctta tatgcagtga actgcatatt gccccgtcaa
     1801 cgtattacca ctgtcagcaa cagcgacatc gtcctgataa acgcagtacc cgtgcgcagc
     1861 gcgatgactg gctgaagaaa gagatactgc gcgtatacga tgagaatcat caggtataca
     1921 gtgtacgtaa agtctggcgt cagttgttac gggaaggtat cagagtggcc aggtgcactg
     1981 tggcacgtct catggcggtt atggggcttg ccggtgttct ccggggtaaa aaggtccgca
     2041 ctaccgtcag ccggaaagcc attaccgcag gcgaccgcgt aaaccgtcag ttcggggcag
     2101 aacgtcctga ccagttgtgg gtggctgatt ttacttacgg tgtgcccgga gttcagggcg
     2161 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
     2221 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
     2281 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
     2341 gagatgatcc tcaaggttaa gtactgaaag gttgaagaac atgaacccgt taatccgcct
     2401 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
     2461 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
     2521 cagcagcgtc tatcacgctt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
     2581 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
     2641 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
     2701 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
     2761 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
     2821 agctggccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
     2881 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
     2941 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
     3001 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
     3061 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
     3121 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     3181 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     3241 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     3301 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     3361 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     3421 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     3481 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     3541 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     3601 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     3661 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     3721 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     3781 gctttatctt tctggggcac aggctcattc gcaaacgcag tcgttatggc gagatgcgag
     3841 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     3901 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     3961 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     4021 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     4081 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     4141 tatttggtaa aaccaaacac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     4201 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacgggga
     4261 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     4321 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     4381 gtccgactac gccctgcctc ttactctact cagcacatgg cagggcttcg tttatgtggc
     4441 gttcatcatt gatgtgttcg ccggatacat cgtgggatgg cgggtctcat cgtccatgga
     4501 aacgacattc gtgctggatg cactggagca ggcagtatgg gaccgtcgtc cgtctggcac
     4561 tatccatcac tgtgataaag gttctctgta tgtatcgctg gcctacacgc agcggcttaa
     4621 ggaagcagga ttactggcat caacagggag cacaggcgac tcgtatgaca acgcgatggc
     4681 ggagagcatc aatggtcttt acaaagcgga ggtaatacac cgtaatagct ggaaaaaccg
     4741 gacagaagtg gaactggcca cactaacgtg ggtggactgg tataacaatc gacgattgct
     4801 gggaaggctg gatcatatcc ctccggcaga agcagaaaaa gcttattatg cttccatcag
     4861 aaacgatgat ctggcagcct gagttcacaa ataaaacaat ctccaggaaa cccggggcgg
     4921 ttcaaatttc gcagagggct ggcttctgat gctgacgcta acgtgctttt cccacctgct
     4981 aaccagaaag taatctgccc agcataacag taaccccatc agcttaaaat caataacgcc
     5041 ctgtaaaacc tgaagtaaca gtccgggaac tcgtatcatg ctaacatatc agtgttgtga
     5101 agcagtcgac aacgagcttc agacggtaca cacgtcagtt catgcagaaa tagcggccat
     5161 atcaggtctg gctgtcagcg aaccacgcag cctgagtaca ttgttgtaaa ttaaacgact
     5221 ggtgattttt aaattttttc tttgccacca ccgtgaacta ataccgaaaa aacagtagtc
     5281 tcaggaagcg agttctgaaa gtcagcttta ttaaaataag ctattagacc gggtaagtaa
     5341 tgtcaacgaa tgaaatgtta tgataattct ggataccata tctgtaagag tgattaatga
     5401 ggaaagtgat gacttttaat tattttatct ccggtaacgt aaaaccagac ttatcgtaaa
     5461 tgaaattatg aagtcctctg ttaacgtcat ggagagacgc atcaaaatac aggatacaat
     5521 gattctcaca atgaaactca tcgtctctgg tgaaattcat atcaacattt accttaataa
     5581 cctgacgcgg gattaactgt gttgtttaag gcaaaggggg gagttgcccg aaggcgaaaa
     5641 cgaaagtaag ttaataataa caggatgaga ggagaaagcg ctcttcccgg aaaataaaaa
     5701 gagcggttca ctcctgtgaa tgcacttcaa ccgtcctggc aaagaatgta ctgttataaa
     5761 aaactccccc agtcagcaga gacaaaaaga taacatgagg tacctcccac ccccttaatt
     5821 tatacacgtg aaatgaactg aaatttatga gaaaatcatg ctaaagatgc tttgatttta
     5881 cttaaaatca cgtgtgatta tcctgactga aggagagata ctatggttaa ggttgtggaa
     5941 gatgaaagaa gcagaatacg atatcttgaa agacgcttaa atgaaaatgg gttttattta
     6001 ccatcaagcc tggccgataa agattattta tcgtatcaga aacggatttt aaacactctt
     6061 atttcacagg gtgttgacac cctaaaaata aataattttc tggctgaaac agaccagcgt
     6121 tacttcgatt ccttaccatc tgagaatgat cttaactggt atcgtaatga tgcccgcgcc
     6181 agtttatggc tgacttgtga attatatgaa atgattaaga taaacggtta tgaaaatacc
     6241 cttacctgtt tatctccgga gtcactacct tctcaccaca gcgtgagggt agatgccata
     6301 cgcaggtgta ttgataactg gcctttcatt ttatacacac catcaaatta cctaaaccag
     6361 aaaagtattg aatggaccac actgctcgaa aaagatgata ttttcagaga ggttaaggcc
     6421 agaaatgttg atatctgctc atggctcaaa aaatatattc aggaaaagac gaatatttcc
     6481 ctgaactatg tctgtggaga atcatctgaa gagattatgg cctggtgtta tgccagttac
     6541 ttcacatgga aaaaaaacaa ccagaattca ccagactcag tagaactgtt tacccggaag
     6601 tttaaaagcg cctgggctac ccaaaaaaac agaataaaaa acagggttga taaaaagctg
     6661 cgccccctga acgtaaatat ttcacaggag gcatatgaca aactgagaaa attatccatg
     6721 aacgagggta tctcaaatga tcgggttatt gaaagtgctc tggacatgat ttaccgtagt
     6781 aaaattaaaa aataaaacca ttcaaaataa tttcagacag atattatctc cgtgaatccc
     6841 ccgccacctt tccggtgtgc ggggt
//
LOCUS       DAZFWP020000217         6832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFWP020000217 DAZFWP020000000
VERSION     DAZFWP020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.218"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..281)
                     /locus_tag="ACHK0Z_RS22990"
                     /old_locus_tag="pgaptmp_004598"
     CDS             complement(<1..281)
                     /locus_tag="ACHK0Z_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HGU4504482.1"
                     /translation="MFGIFGKKARRAAVEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQILRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREI"
     gene            complement(471..680)
                     /locus_tag="ACHK0Z_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             complement(471..680)
                     /locus_tag="ACHK0Z_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504483.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            complement(733..957)
                     /locus_tag="ACHK0Z_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             complement(733..957)
                     /locus_tag="ACHK0Z_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504484.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            complement(1048..1124)
                     /locus_tag="ACHK0Z_RS23005"
                     /old_locus_tag="pgaptmp_004601"
     tRNA            complement(1048..1124)
                     /locus_tag="ACHK0Z_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Arg,seq:tct)
     gene            complement(1138..1214)
                     /locus_tag="ACHK0Z_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     tRNA            complement(1138..1214)
                     /locus_tag="ACHK0Z_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Arg,seq:tcg)
     gene            complement(1222..1297)
                     /locus_tag="ACHK0Z_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     tRNA            complement(1222..1297)
                     /locus_tag="ACHK0Z_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1262..1264),aa:Ile,seq:cat)
     gene            complement(1802..2554)
                     /gene="ydfT"
                     /locus_tag="ACHK0Z_RS23020"
                     /old_locus_tag="pgaptmp_004604"
     CDS             complement(1802..2554)
                     /gene="ydfT"
                     /locus_tag="ACHK0Z_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4504485.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSSLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
     gene            complement(2568..3617)
                     /locus_tag="ACHK0Z_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             complement(2568..3617)
                     /locus_tag="ACHK0Z_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4504486.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            complement(3619..3888)
                     /locus_tag="ACHK0Z_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             complement(3619..3888)
                     /locus_tag="ACHK0Z_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504487.1"
                     /translation="MAHDSRLHNSAALASRQGRCSHVFKTDWFQHPLCTEEQAEWLIQ
                     CYRRRGYEFQKDLSFDRRHWIISARLPYSERPPRPSRTFQQRIWR"
     gene            complement(3942..4136)
                     /locus_tag="ACHK0Z_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             complement(3942..4136)
                     /locus_tag="ACHK0Z_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504488.1"
                     /translation="MKIPASLIPGFYESTRPVVVYRNSDGSFQCGFVMRSNEVVVSLS
                     LLSEVRELAGLPVDDIQKQL"
     gene            4393..4764
                     /locus_tag="ACHK0Z_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     CDS             4393..4764
                     /locus_tag="ACHK0Z_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4504489.1"
                     /translation="MRIFKNAWFERFARKHRISDKSLRKIVEQADKGIISANLGSGVI
                     KQRLARSGGGKSGGYRTIIFYRVAEKAFFIYAYAKNERENITAIEENAFRKAAPHVLN
                     LTDEQLAQLIQQGQITEVPNE"
     gene            4757..5074
                     /locus_tag="ACHK0Z_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             4757..5074
                     /locus_tag="ACHK0Z_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4504490.1"
                     /translation="MSKNYRSDALASVHEMMESLHDIGAITKQTMREFDETCLQPAPV
                     MSPERIRALREREHLSQPVFARYLNVSKNLISDWERGVKRPGGAALRLLSVVEKNGIQ
                     VIS"
     gene            complement(5177..5332)
                     /gene="hokD"
                     /locus_tag="ACHK0Z_RS23050"
                     /old_locus_tag="pgaptmp_004610"
     CDS             complement(5177..5332)
                     /gene="hokD"
                     /locus_tag="ACHK0Z_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4504491.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5604..6155)
                     /locus_tag="ACHK0Z_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             complement(5604..6155)
                     /locus_tag="ACHK0Z_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU4504492.1"
                     /translation="MTTQVTVKNLSPVTYNQIPVITTELLARLYGTEAIRIQQNHHEN
                     KSRFIEGKHFFKAVGDELKNLRLVLNESQNEVKISPKTRTLILWTERGAARHAKMLET
                     DQAWEVFEKLEDSYFNQYEKERAMGKMNIDFHLAIRDDKTAFIRSYAPGQLVTVEEAL
                     ALLKSRGWLVMPRDELAGKLMEL"
     gene            complement(6576..6755)
                     /locus_tag="ACHK0Z_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /pseudo
     CDS             complement(6576..6755)
                     /locus_tag="ACHK0Z_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(6752..>6832)
                     /locus_tag="ACHK0Z_RS23065"
                     /old_locus_tag="pgaptmp_004613"
     CDS             complement(6752..>6832)
                     /locus_tag="ACHK0Z_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504493.1"
                     /translation="VCAALRELNKCRDILRDITATREQQR"
BASE COUNT         1734 a         1808 c         1517 g         1773 t
ORIGIN      
        1 tcaatttcac gcaatgccgc tcgacgacca attttaaagt tcgtatccag ctgaccgata
       61 atggttgcgc taatcgcatt aatttctgac gtaaacgcgg acaacgcagg ctggttacgc
      121 aagatctgct cgatcttcgc tttctctgaa gcctcacatt caccatctgc ataggccacc
      181 agataggcag cattaataac cgcctgtgcc agatcacgtt tctcaaactt tttaatttcc
      241 actgccgctc ggcgggcttt tttaccaaaa ataccaaaca tcgtgacgtt cctttgggtg
      301 ggtgagccaa cgcccgggag cgatctgccc acagagaaag tcacactgac cactccgtaa
      361 gctcaccccc gaaaggctct gtggttgata tgcgccgggc gtggcgcgga tacaaaaaag
      421 gccgccaata gcgacctcag ttacgggatt attctggggt taaacgactg ttactccccc
      481 cagacaaaat catcacttcc tgttcgatgc gagccatagt gaacctcgta cttatctccc
      541 atctttcttg cttccgtttc tgcgtcttcc tctgtcgcaa aaaccccaac aagatgccag
      601 ggcgagcttc ttaccacagc ccaaccttta acccatcctt tgttgtcctt atcttccatt
      661 aacacttcag aaacaaacat atttatctcc ttgtgggtac ccagagatat tttatgattg
      721 ctcccggtca gatcaataaa gtggcttcaa ttttgcctta atgatcaaat cagggtgatt
      781 gacggaatcg tacaccacct caatattttc atccgtggcg tcgataagat attcttttac
      841 ataaggacct gttgattttc catgaaatac atcttcaaca agtacactct ccccctgaac
      901 aacacgaaaa ctaacttctg tttcgaacgg accaatcgtc accatcagtt ttttcacata
      961 gcctcctgat aagcactcga tttattagtt aatggtgtaa cgcagataca aaaaaaggcc
     1021 cgcaaaagcg agccaagtaa ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg
     1081 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
     1141 accgccatcg ggaacttgaa ccccgcacag ccagcttcga aggctgacgc tctatcccga
     1201 tgagctaatg gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat
     1261 tatgagtcgc tcgctctcac cactgagcta aagggccggg cgcaggataa taacggtacg
     1321 taactaattc tgcaatatca tccgttctga ctgactacat tctgaacttc cctgaccgtc
     1381 tgctcaaaac gcccgctctc cagctcaacg ccaattgcac gacgccccag cgccattgct
     1441 gctttgacgc tacggacata aaaaagccag ccactggggg aggctggcga aactcgtaga
     1501 gcaaaagtgt tgttacacta acttcgtcac agggttatcc tgcaatactc aaaatacaca
     1561 atatttacaa aactaatagt atacaaggcg atctttaaga ttttgttatt aattgtggtc
     1621 gcaccttcct ttctgtgtac tttccgtata gctcacagga ttctgggtac aaaaaaaccc
     1681 gcgcatcggc gggttcttaa atcttatcaa cggtagacat acaaagccca tcattgggaa
     1741 aatcttatcc atattttttg aaaaatgcaa gcatcatgtc gtcatcttcg gcgaaaacca
     1801 tttatcttgt cacctttctc aattgtatct ctgcatatgc ttcttcctgc cagcactttg
     1861 taaccagttt atcaatgaca tctgcatatc ctttgtacca ctgataatcc gtcaggtctg
     1921 gtaccagctt ctggacatga agccgcgcca gtgtggttgg taaactgcta aaccggtttc
     1981 cattgcaacg cccacaaacc ttataaacag gcgtgccatg aagccgggtt cttttttcat
     2041 ccaggacaat acctttaccc ttgcaccctc tgcatgctgt gctgacttct cccttaccat
     2101 gacaatgctg acatagttcc ttcacccact cttccttaat aacagattcc ccgcttctgg
     2161 agtgtttcac cacctcgcgc aatacattat gaaatccagt accagcacaa tgctcacagc
     2221 gagccttact tgccgcagac ctggaataat cagcaaaggc aaaattcaca aggtaaggaa
     2281 tgatctgtag ccgggtttct tcactcaatt tattcaatgt cgggttatcc agtgccatcg
     2341 cgtaattgag cagaccttca atcgcaaact gaggatcctg aacaccaact tttgccagga
     2401 ataaagcaaa cccaagcggt gcttttgact gcaccatccc ctgcgcagcc atcacatccg
     2461 taattgttaa accaccagag cctgtcgccg gtgcgtcatc gctcagtttt ggagatttcg
     2521 gggagtaata tttcggtaag gcttcaaggt tcatgctcgt tctccactta cgccagtacg
     2581 ccaattgcca gcgcacgatc gataaaacga aatatcagct ccagctgaga gccatacttc
     2641 tcttcaaatg ccacggtatc cgcatgaagc tcgttgtgat gctcccgaca cagcggtagc
     2701 gtgaaaatat cgtgagattt tgtccccatt ccgccctgac catgaccaat caggtgatgg
     2761 ggatcgtcgg ctggcttacc acaacacgca cacggctgtg tctttaccca gcgtgtgtat
     2821 ttctcattta cccagcggcg acgtttaggt cgtttcatga aggattccgg agactccgga
     2881 tcaacggcaa tgctgaccac cgtcttttcc tgtggtgggt tctgttgctg gtgggtgtga
     2941 gacggtagcg caatattttt tgtgcgctgc ttcagtatgc tggtggcggt ctgttctccc
     3001 ggtatgatgt cgctttcacg gtatacggag cggatttttt ccgcacgcaa ccccagcgaa
     3061 cgacgtaata ccgcttccgg tagcgcgtcc gccacctgat tgcggaccgc ccaccaggat
     3121 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
     3181 atcatccatg ctgacaggtt ttgttgagca agttgctcga gtgattctga tgtctggtcg
     3241 cgcagctggt tgtcacagtg ccagcacaac accatcgcgc cggtaccata acggtgaatg
     3301 actgtttcgc tgtgatgata atcgccgtgt ggccactggc aggatttcac gtgacgtaat
     3361 aaccagtcag acagtgcacc tgcaccacct gctgcacgaa taacccgctc atcgctgaaa
     3421 aatggcagta atgttttatc ctctgccagc ggctggcgaa cggcaggaac gactccggac
     3481 ggcagaccgc gcatgttttt cggttccggc tccaccaata ttctgccgtt atggaatact
     3541 gacattgatt cacggcctgg cttaacgata accagaccga gttccggtac cagaacaggt
     3601 cgaagtaata cccgcacgtt acctccagat gcgctgctgg aatgtgcggg acggacgcgg
     3661 tgggcgttcg gaataaggga gcctggcgga gattatccag tgacgacgat cgaagctgag
     3721 atctttctga aactcgtaac cacgcctgcg gtaacactgg atcagccatt cggcctgttc
     3781 ttcagtgcat aggggatgct ggaaccagtc ggttttaaat acgtgcgaac accgtccttg
     3841 cctactggca agggcggcag aattgtgcaa tctgctatcg tgcgccatcg ggatctccgg
     3901 tggcacggtg tttctcagcg acggttcaag tcagcctgat tttatagctg cttctgaata
     3961 tcatcaacag gtaatcctgc caactctctt acctcagaaa gaagagaaag gcttacaaca
     4021 acctcattac ttcgcataac aaaaccacat tgaaaagaac catcactgtt tctgtaaaca
     4081 acaaccgggc gcgtgctctc ataaaacccc gggatcaaac tggctgggat tttcacaaca
     4141 cctcctgacg taaaggaaat gaaatgcatt atcgcttctc tcggactata accatgaaaa
     4201 gagacgcatt tcactcagta aatctgagga ttttatgcgc aagaacaatg actttttctg
     4261 tctgccgttt atacaatctg aattacgtct gttttttgaa caccgataaa tcagcaagca
     4321 ttctcaaata aagattgcct ccatagtcca catggtgtaa cactatgtgt tatataaaat
     4381 gcagaggcag gtatgcgaat tttcaaaaac gcctggtttg aacgtttcgc tcgaaaacat
     4441 cggatttccg ataaatcgct gcgcaaaatc gtggagcagg ccgataaggg gatcatatcc
     4501 gcaaatttgg gtagtggtgt cattaaacaa agattagccc gaagtggtgg cggaaaatca
     4561 ggcggttacc ggacaataat tttttaccgc gttgcagaaa aagccttttt catctacgca
     4621 tacgcaaaga atgaacgaga gaatatcact gctatagagg aaaatgcttt tcgaaaagcc
     4681 gctccccatg tcctcaatct tactgatgaa cagctggcac aattgattca acaaggccag
     4741 atcacggagg tacccaatga gtaaaaatta ccgcagtgat gcacttgcat ctgtacatga
     4801 aatgatggag tcactccatg atatcggtgc aatcacaaaa caaactatgc gcgaattcga
     4861 tgaaacttgt cttcagcctg cgccggtaat gtctccggaa aggatccgtg cactgcgaga
     4921 acgagagcat ctgtctcaac ctgtttttgc cagatacctc aacgtcagta aaaacctgat
     4981 atcagactgg gaacgaggag tgaaacgccc gggaggtgca gctcttcggc ttctttcagt
     5041 tgtcgagaaa aacgggatcc aggtaatatc ctgatattct aatacaacaa aacccgccga
     5101 agcaggttaa gtgcgggtgc gttgaggatg cctgacacat cagaggtggc gagggatttc
     5161 tccctcgcca ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg acctccgtct
     5221 ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc gtcactatga
     5281 cggttaaaca gatgacaatc agggcgatta acatcgcctt ttgctgcttc atagcctgct
     5341 tctccttgcc tttcggcacg taagaggcta acctacatgt gcaaagcatg aaattggcct
     5401 cagattaatg ttaagcgtcc tgcaagacgc gtaatgttaa ctggggcttt tctctgtctg
     5461 ccttacggcg gcatgcccga ggcagacagc ctcaagcacc cgcagcaatt ctacttaact
     5521 cttctttccc cgcaaatcat tttatccccg atggtaatgt tctcccgata tgggaattcc
     5581 catatcaagg ttaactcaat cggttaaagc tccattaatt ttccggccag ttcatctcgt
     5641 ggcattacca gccatccgcg cgatttaagc aaagccaggg cttcttcaac cgtcaccagc
     5701 tggccaggcg cataacttcg gatgaaggcg gttttatcgt cacggatcgc caggtgaaaa
     5761 tcgatattca tttttcccat agcccgctct ttctcgtact ggttgaagta actgtcttcg
     5821 agtttttcga atacttccca cgcctgatcg gtttcgagca tttttgcatg acgggctgct
     5881 ccgcgttctg tccagaggat gagagtgcgg gttttgggag aaattttcac ctcattttgc
     5941 gactcgttta aaactagtcg caaatttttg agctcatcac caacagcttt aaagaagtgt
     6001 tttccctcaa taaatcgaga tttattttca tggtgattct gctgtatacg gattgcttct
     6061 gttccgtaaa ggcgggcgag taactcagtt gtgattacag gaatctggtt ataagtgaca
     6121 ggggaaaggt ttttgacagt aacttgagtc gtcatgataa cgccctctgg ttgatgaatt
     6181 taactatcac caccttcagg tctcaatcat caggtggcga gacgtacagg gttgagacta
     6241 ccggatcaac caaccggcca gcctttcggc tgccccatac gcctcaccat aattcagatg
     6301 tgcgtgcgca tacgacaata aaaaacacgc tcgcggcgtg tgtctgtcgc ggttgaatat
     6361 ccggggtctc aatcccgacg gtcaactcga ccgtgcggtg aatatagccc cggattagta
     6421 attacgtcaa ccccagcggc aaatcgaata aaccaccagc gctaccgcca ttgcaactcc
     6481 tgccgttacg aatgcctcag gccaggtcat cgtaaactat cctcagcgcc aatcagtccg
     6541 tttcgcttca ggcagtccat cgctttatac ggtaatttgg ctgacaggcg aaaatcaccc
     6601 tgcagcatca ggcttattcc cttatcccgg gctttcgctc tgaccgctgc ctcgctacga
     6661 ccaatcagac tgccgatact ttcgacagtc atcgttcccg cgcactgccg gagtatcaga
     6721 atttcagccc ggcaccacgt cttccaccca ctcaccgctg ctgttctctg gtggcggtaa
     6781 tatcccggag aatatcccgg cacttgttca gctcccgcag cgcggcgcag ac
//
LOCUS       DAZFWP020000218         6826 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFWP020000218 DAZFWP020000000
VERSION     DAZFWP020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.219"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1976
                     /gene="acnA"
                     /locus_tag="ACHK0Z_RS23070"
                     /old_locus_tag="pgaptmp_004614"
     CDS             <1..1976
                     /gene="acnA"
                     /locus_tag="ACHK0Z_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU4504494.1"
                     /translation="AAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKH
                     GVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQ
                     VELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFA
                     ASNELEVNATHKNRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAK
                     KAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGP
                     LPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINL
                     AAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVT
                     RSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIK
                     PDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHL
                     PDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFER
                     IHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQ
                     EVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2040..2630)
                     /gene="ribA"
                     /locus_tag="ACHK0Z_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             complement(2040..2630)
                     /gene="ribA"
                     /locus_tag="ACHK0Z_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU4504495.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2800..3564
                     /gene="pgpB"
                     /locus_tag="ACHK0Z_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             2800..3564
                     /gene="pgpB"
                     /locus_tag="ACHK0Z_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU4504496.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3713..4021
                     /gene="lapA"
                     /locus_tag="ACHK0Z_RS23085"
                     /old_locus_tag="pgaptmp_004617"
     CDS             3713..4021
                     /gene="lapA"
                     /locus_tag="ACHK0Z_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU4504497.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4028..5197
                     /gene="lapB"
                     /locus_tag="ACHK0Z_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             4028..5197
                     /gene="lapB"
                     /locus_tag="ACHK0Z_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU4504498.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQIYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5390..6127
                     /gene="pyrF"
                     /locus_tag="ACHK0Z_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             5390..6127
                     /gene="pyrF"
                     /locus_tag="ACHK0Z_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU4504499.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CSLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6127..6453
                     /gene="yciH"
                     /locus_tag="ACHK0Z_RS23100"
                     /old_locus_tag="pgaptmp_004620"
     CDS             6127..6453
                     /gene="yciH"
                     /locus_tag="ACHK0Z_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU4504500.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6579..6797)
                     /gene="osmB"
                     /locus_tag="ACHK0Z_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             complement(6579..6797)
                     /gene="osmB"
                     /locus_tag="ACHK0Z_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU4504501.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         1674 a         1613 c         1828 g         1711 t
ORIGIN      
        1 aagccgcaat gttaggccag ccggtttcca tgcttatccc ggacgtagtg ggctttaaac
       61 ttaccggtaa attacgtgaa ggtattaccg ccacagacct ggttctcact gttacccaaa
      121 tgctgcgcaa acatggcgtg gtggggaaat tcgtcgaatt ttatggtgat ggtctggatt
      181 cactaccgct ggcggatcgc gccaccattg ccaatatgtc gccagaatat ggtgccacct
      241 gtggcttctt cccaatcgat gctgtaaccc tcgattacat gcgtttaagc gggcgcagcg
      301 aagatcaggt tgagttggtc gaaaaatatg ccaaagcgca gggcatgtgg cgtaacccgg
      361 gcgatgaacc gatttttacc agtacgttag aactggatat gaatgacgtt gaagcgagcc
      421 tggcagggcc aaaacgccca caggatcgcg ttgcactgcc cgatgtacca aaagcatttg
      481 ccgccagtaa cgaactggaa gtgaatgcca cgcataaaaa tcgccagccg gtcgattatg
      541 ttatgaacgg acatcagtat cagttacctg atggcgctgt ggtcattgct gcgataacct
      601 cgtgcaccaa cacctctaac ccaagtgtgc tgatggccgc aggcttgctg gcgaaaaaag
      661 ccgtaactct gggcctcaag cggcaaccgt gggtcaaagc gtcgctggca ccgggttcga
      721 aagtcgtttc tgattatctg gcaaaagcga aactgacacc gtatctcgac gaactggggt
      781 ttaaccttgt gggatacggt tgtaccacct gtattggtaa ctctgggccg ctgcccgatc
      841 ctatcgaaac ggcaatcaaa aaaggcgatt taaccgtcgg tgcggtgctg tccggcaacc
      901 gtaactttga aggccgtatc catccgctgg ttaaaactaa ctggctggcc tcgccgccgc
      961 tggtggttgc ctatgcgctg gcgggaaata tgaatatcaa cctggctgct gagcctattg
     1021 gtcatgatcg taaaggcgac ccggtttatc tgaaagatat ctggccatcg gcacaagaaa
     1081 ttgcccgtgc ggtagaacaa gtctccacag aaatgttccg caaagagtac gcagaagttt
     1141 ttgaaggcac agcagagtgg aaggaaatta acgtcacacg atccgatacc tacggttggc
     1201 aggaggactc aacctatatt cgcttatcgc ctttctttga tgaaatgcag gcaacaccag
     1261 caccagtgga agatattcac ggtgcgcgga tcctcgcaat gctgggggat tcagtcacca
     1321 ctgaccatat ctctccggcg ggcagtatta agcccgacag cccagcgggt cgatatctac
     1381 aaggtcgggg tgttgagcga aaagacttta actcctacgg ttcgcggcgt ggtaaccatg
     1441 aagtgatgat gcgcggcacc ttcgccaata ttcgcatccg taatgaaatg gtgcctggcg
     1501 ttgaaggggg gatgacgcgg catttacctg acagcgacgt agtctctatt tatgatgctg
     1561 cgatgcgcta taagcaggaa caaacgccgc tggcggtgat tgccgggaaa gagtatggat
     1621 caggctcaag ccgtgactgg gcggcaaaag gtcctcgtct gcttggaatt cgtgtggtga
     1681 ttgccgaatc gtttgaacga attcaccgtt cgaatttaat tggcatgggc atcctgccgc
     1741 tggaatttcc gcaaggcgta acgcgtaaaa cgttaggact aaccggggaa gagaagattg
     1801 atattggcga tctgcaaaat ctacaacccg gtgcgacggt tccggtgacg cttacgcgcg
     1861 cggatggtag ccaggaggtc gtaccctgcc gttgtcgtat cgacaccgcg acggagttga
     1921 cctactacca gaacgacggc attttgcatt atgtcattcg taatatgttg aagtaacaag
     1981 tatttgcttg ccggttattt tgcttccggc aagcaaacta attacacaat gcaagagggt
     2041 tatttgttca gcaaatggcc cattttctcg gctttagtat cgagataatg ttcgttattg
     2101 gggttacgac ctacaatcaa tggtacgcgt tcaacaatat taatccctgc ttcggtcaga
     2161 atttcgactt ttttcgggtt attggttaac aagcggactt cattgacgcc aaggagtttg
     2221 aacatatcag cgcaaagagt gaagtcgcgc tcatcagcgg cgaagcctaa ctggtggtta
     2281 gcctctacgg tatcgtaacc ttgatcctgc agtgcgtaag cgcggatttt attcagcaga
     2341 ccaatgttac gaccttcctg acggtgatac agcaaaatac cacggccttc ctcggcaatt
     2401 tgcgtcaatg ccgcttcgag ctggaagcca caatcgcagc gcaagctgaa cagggcgtca
     2461 ccggtcagac attcggaatg gacgcgcgca agtaccgggg tatgcccgga aatatcgcca
     2521 tagactagcg cgacatgatc gtgtccggtt gccagttctt caaatcccac catcaggaaa
     2581 tcgccccatg gggttggcag tttggcttct gccacacgtt taagctgcat gaaattctcc
     2641 agataatgct ggttctgtat tggcttattt tgccataacg agaaggggtt cacctaatca
     2701 cgacgcgtcg atcgttcacg gaatggcaca aatctgtcaa tttttcctgg aactggcgtt
     2761 ttcagttatg attgtgggac ttatcaaaaa ggagaggcca tgcgttcgat tgccagacgt
     2821 accgcagtgg gagctgcact attgcttgtc atgccagtag ccgtatggat ttctggctgg
     2881 cgttggcaac ctggagaaca aagttggcta ctaaaagcgg ctttttgggt tactgaaact
     2941 gtcacccagc cctggggcgt cattacacat ttgattttat tcggctggtt tctctggtgt
     3001 ctgcgttttc gcattaaggc tgcctttgta ttatttgcca ttctggcggc cgcaatcctt
     3061 gtgggacaag gcgttaaatc ctggatcaaa gacaaagtcc aggaaccacg accttttgtt
     3121 atctggctgg aaaaaacaca tcatattccg gttgatgagt tctacacttt aaagcgagca
     3181 gaacgcggaa atttagtgaa agaacagttg gctgaagaga aaaatatccc acaatatttg
     3241 cgttcacact ggcagaaaga gacggggttt gcctttcctt ccggtcacac gatgtttgct
     3301 gccagttggg cactgctggc cgttggtttg ctgtggccgc gtcggcgaac gttaaccatt
     3361 gctatcttgc tggtctgggc aacgggagtc atgggaagcc gcctgctgct cgggatgcat
     3421 tggccacgcg atctggtagt agctacgttg atttcgtggg cgctggtggc ggtggcaacg
     3481 tggcttgcgc aacgaatttg tgggccatta acaccacctg cggaagaaaa tcgcgaaata
     3541 gcgcaacgag aacaagaaag ttaacgctgg ttgattttcc gaatttagcc cttaaatcat
     3601 caacaatgcg cgtggatgcc atttcgcaga cggcgcgaaa atggtacttt aaagggctat
     3661 tgcggtaagt tgaccataat ttattcgctc taaccacata acgggaagta atgtgaaata
     3721 tttactcatt ttcttactgg tgttagcgat cttcgtgatt tcggtcacgt tgggtgcgca
     3781 gaacgatcaa caggtgacgt ttaattatct gttagcgcaa ggggagtacc gtatttccac
     3841 attgctggcg gtattgtttg ctgcggggtt tgctatcggt tggttgattt gtggcctgtt
     3901 ctggctgcga gttcgtgttt ccctggcgcg cgctgaacgt aaaataaagc gactggaaaa
     3961 ccagctttca cccgcgactg acgtggctgt agtgccgcac tcgtcagcgg cgaaggaata
     4021 actttctatg ctggagttgt tgtttctgct tttgcctgta gccgctgcct atggctggta
     4081 tatgggccgc agaagtgcgc aacaaaataa gcaagatgaa gccaaccgct tgtcgcgtga
     4141 ttacgtagcg ggggttaact tcctgcttag taatcaacag gataaagcgg tagacctgtt
     4201 tctcgatatg cttaaagagg atacaggcac cgttgaagcc caccttacgc tcggaaacct
     4261 gttccgttcg cgtggcgaag ttgatcgcgc tattcgcatc catcagaccc taatggaaag
     4321 cgcctcgctg acctatgaac agcgtctgtt ggcgattcaa caactagggc gtgattacat
     4381 ggccgccggg ttatacgacc gcgcggaaga catgttcaat cagctgaccg atgaaactga
     4441 cttccgcatt ggcgctctgc aacagttgct acaaatctac caggctacca gtgagtggca
     4501 gaaagcaatt gatgttgccg aacgcctggt gaagctgggt aaagataaac agcgcgtcga
     4561 aattgcccat ttctactgtg agttagccct gcagcatatg gccagcgacg atctcgatcg
     4621 tgccatgacc ttgctaaaaa aaggggcggc ggcagataaa aacagcgccc gcgtatccat
     4681 catgatggga cgcgtgttta tggcgaaagg agaatacgcc aaagccgtcg aaagtttgca
     4741 acgcgtgata tcccaggaca gagaactggt cagcgaaacg ctggaaatgt tgcaaacctg
     4801 ctatcagcag ttgggtaaaa ctgccgaatg ggcagaattc ctgcaacgcg cggtggaaga
     4861 gaacaccggt gccgatgctg aattgatgct tgcggatatc atcgaagcgc gcgacggtag
     4921 tgaggctgca cagatctata ttacgcgcca gcttcagcgt catccgacca tgcgtgtgtt
     4981 ccataagtta atggattacc acttaaatga agcggaagaa gggcgtgcca aagagagcct
     5041 gatggtgctg cgtgacatgg ttggcgaaaa ggtacgtagt aagcctcgtt atcgctgcca
     5101 gaaatgtggt tttaccgcat acaccctcta ctggcattgt ccgtcttgtc gggcctggtc
     5161 aaccattaaa ccgattcgcg gtcttgatgg cctgtaattt ttaaaaaaat ccgactttag
     5221 ttacaacata ctagttattg atgttccatt gtgctccggc aacgacggag cagaaaaagc
     5281 ctgccagggg aaaaatcgca actgttaatt ttttatttcc acgggtagaa tgctcgccgt
     5341 ttacctgttt cgcgccactt ccggtgccca tcatcaagaa ggtctggtca tgacgttaac
     5401 tgcttcatct tcttcccgcg ctgttacgaa ttctcctgtg gttgttgccc ttgattatca
     5461 taatcgtgat gacgcgctgt cctttgtcga caagatcgac ccacgcgatt gtcgtctgaa
     5521 ggtcggcaaa gagatgttta cattgtttgg gccacagttt gtgcgcgaac ttcaacagcg
     5581 tggttttgat atctttcttg acctgaaatt ccacgatatt cctaacactg cagcgcacgc
     5641 tgtcgctgct gcagctgact taggcgtgtg gatggtgaat gttcatgcct ctggtggggc
     5701 gcgtatgatg accgcagcgc gtgaggcact ggttccgttt ggcaaagatg caccgctttt
     5761 gattgctgtg acagtgttga ccagcatgga agccagcgac ctggtcgatc ttggcatgac
     5821 actgtcacct gcagattatg cagaacgtct ggcggcactg acgcaaaaat gtagccttga
     5881 tggtgtggtg tgttctgctc aggaagctgt gcgctttaaa caggtattcg gtcaggagtt
     5941 caaactggtt acgccgggca ttcgtccgca ggggagtgaa gctggtgacc agcgccgcat
     6001 tatgacgcca gaacaggcgt tgtcggctgg tgttgattat atggtgattg gtcgcccggt
     6061 aacgcaatcg gtagatccag cgcagacgct gaaagcgatc aacgcctctt tacagcggag
     6121 tgcatgatga gtgattccaa cagccgtctg gtctactcaa cggaaaccgg acgtattgat
     6181 gagcccaaag cggcccctgt acgccctaaa ggcgacggtg tggtgcgtat tcagcgtcag
     6241 accagtggac gtaaaggtaa gggcgtttgc ctgattaccg gtgtcgatct cgatgatgcc
     6301 gaactgacaa aacttgcagc ggaactgaag aaaaaatgcg gctgcggcgg agcagttaaa
     6361 gatggagtta ttgaaatcca gggcgataag cgtgatttat taaaatcact acttgaagcg
     6421 aaagggatga aggtaaaact cgcaggcggt taacataaaa agccacggat atatccgtgg
     6481 ctttcgaata ttttactgtg cgtattattc agttatttcg tactgaacag aatcaagcgt
     6541 attataattg cgagtcgata atcgacattt atttacgatt atttaccgac ctggtgacca
     6601 ataacaccac cgacagctgc accacctaat gtacccaacg tactgccatc ggtcagtact
     6661 gcaccgccta atgcccctgc acccgcgccg attgcggtgt tgcggtcccg tttagaccag
     6721 ttagaacagg cactcagaga cattgccaaa gtaattgcca gaacagccgc ggtcattttt
     6781 ttgctcgtta caaacataat actctctcct gaatttatga ttcacg
//
LOCUS       DAZFWP020000219         6788 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFWP020000219 DAZFWP020000000
VERSION     DAZFWP020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.220"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            62..1492
                     /locus_tag="ACHK0Z_RS23110"
                     /old_locus_tag="pgaptmp_004622"
     CDS             62..1492
                     /locus_tag="ACHK0Z_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4504502.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLEVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1533..2465)
                     /locus_tag="ACHK0Z_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             complement(1533..2465)
                     /locus_tag="ACHK0Z_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4504503.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2829..5615
                     /gene="polA"
                     /locus_tag="ACHK0Z_RS23120"
                     /old_locus_tag="pgaptmp_004624"
     CDS             2829..5615
                     /gene="polA"
                     /locus_tag="ACHK0Z_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU4504504.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5997..6629)
                     /gene="yihA"
                     /locus_tag="ACHK0Z_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             complement(5997..6629)
                     /gene="yihA"
                     /locus_tag="ACHK0Z_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU4504505.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
BASE COUNT         1937 a         1533 c         1648 g         1670 t
ORIGIN      
        1 ggacattatt tcagcataag tggatattca aagttttgct gttttatcag ggaatattta
       61 tatgatacgt aagtcagcta caggtgttat tgttgcgtta gccgtaatct ggggtggtgg
      121 cacatggtac acaggtacac aaattcagcc tggtgtcgaa aaatttatta aagattttaa
      181 cgatgctaaa aagaaaggtg aacatgccta cgatatgacg ttaagttatc aaaattttga
      241 caaaggcttt tttaattccc gttttcaaat gcaaatgaca ttcgataacg gtgcacccga
      301 tctcaatatc aagccaggcc agaaagttgt atttgatgtg gatgttgagc acggtccgtt
      361 gcccatcaca atgttaatgc atggtaatgt catcccagca ctggcagcgg caaaagtgaa
      421 cttagtgaat aatgaactga cacaaccgct atttatcgcc gcgaaaaata aatcgcccgt
      481 ggaagcgaca ttgcgattcg cgtttggtgg ctcattctct acgacattag aggttgcccc
      541 tgcagagtat ggaaagtttt cttttggtga gggccagttt acttttaatg gtgatggtag
      601 ttcattgtct aacctggata ttgaaggcaa agtcgaagat attgttctgc aattatcacc
      661 aatgaacaaa gtaacggcaa aaagttttac cattgattct ctggcgcgat tagaagaaaa
      721 gaaatttccg gttggtgaaa gcgagtcgaa atttaatcag attaacatta tcaatcacgg
      781 ggaagacgtt gcccaaatcg atgcttttgt tgcaaaaacc atgctggatc gcgttaaaga
      841 caaagattat atcaatgtca atctgaccta cgaacttgat aagttaacaa aagggaatca
      901 gcaactcggt agtggtgagt ggtcattgat tgctgaatct attgatccct cagcagtgcg
      961 ccaatttatc atccagtata acattgctat gcagaagcag cttgctgtac accctgagtt
     1021 agcaaacgat gaagttgctc tgcaagaagt gaatgctgca ttgttcaaag agtatttacc
     1081 gttattacaa aaaagtgagc cgaccattaa acaaccggta agatggaaga acgcactcgg
     1141 cgaactaaat gccaatctgg atatcagtat tgccgaccca gccaaatctt catcatccac
     1201 aaacaaagat atcaaatcgc tcaattttga tgtgaagtta ccgcttaatg tcgtcacaga
     1261 aaccgcaaaa cagcttaatt tatctgaagg aatggatgcg gaaaaagcgc aaaagcaggc
     1321 tgataaacaa atcagcggga tgatgacatt gggtcagatg tttcagttaa tcacgattga
     1381 caacaatacc gcctcgctgc agctgcgtta tacaccgggt aaagttgttt ttaacggaca
     1441 ggagatgagc gaagaagaat ttatgtctcg tgccggacgt tttgttcatt aaaatactca
     1501 ctcgcctctg ctcattcaga ggcgagtaaa acttacttat tctgacgctg tgatgacatg
     1561 agagacgtca gaagccggtc tttttcctgc cacaatgagt tcagccattg ctgaaaatgg
     1621 cgcttaaagc ttttgtcatt gatgtagtcg ccatgtaact catcggcaat aggctgtaaa
     1681 tccacatgta cgacaatccg cgtcagttta ccgcttaaca tatcgaagaa cggctgacgg
     1741 ttattgtccg gataacacag tgtaacatta agcagtttat cgaattgttt gcccagtaca
     1801 tttagcgcca tcgcaatgcc tgccgccttt ggtggcaaca agttttgaaa agtggagtgg
     1861 gtttgctgat gtttttcttg cgtgaaacgg gagccttcaa cgaaattgac aatggtggtg
     1921 ggatgcaggc gaaacttttc acaagaacgc cgagtggttt caacatcttt accacgtcgc
     1981 tccggatgac gtaataaata agcgcgggaa taacgcttca taaacggcat atccagcgac
     2041 cagcacgcca ggccaagaaa tggcacccag gccagctgtt gcttgaggaa atatttattc
     2101 atcggaatgt gcttacgaaa cagcacgcac agtacgacaa tatctgccca actacggtgg
     2161 ttacaaataa gcagatacca gttcttctta cttaaccctt ccagcccgtg aacttcccat
     2221 tgcaggtgtg ggttcagatg cagtaatacc gccagacctt cacaccagca atacatcata
     2281 aaatcacaaa aacgcgaaac ctttcgccaa atgactggca caggtagcaa tagttttaca
     2341 atcccggcaa tgatgatcgg gacggaacaa aaaatagtga ccaaaatggt caatacgata
     2401 ctcagcaaaa gggttatcgc agcgagtatt ctcgtcataa tgaatttatt caaaaggtta
     2461 gccatagaca gtgcgctaaa gaagcgcaag gcgcagattt tagcagaaaa cgacccaaat
     2521 aacggatgat ccttaaggag aaaaataatt catatctatc cacattagaa aaaatcccat
     2581 tatctcaatt attagggatg gatttatttt taactgcatg aaaaacaaag acaaacatca
     2641 tgctgtaaaa agcatgataa taaattaaaa gcgatgtaaa taatttatgc acaaagttat
     2701 ccacatgacg atttgcgagc gatccagaag atctacaaaa gattttcacg aaaagcggtg
     2761 aaaaactcat gttttcatcc tgtctgtggc atcctttacc cataatctga taaacaggca
     2821 cggacattat ggttcagatc ccccaaaatc cacttatcct tgtagatggt tcatcttatc
     2881 tttatcgcgc atatcacgcg tttcccccgc tgactaacag cgcaggcgag ccgaccggtg
     2941 cgatgtatgg tgtcctcaac atgctgcgca gtctgatcat gcaatataaa ccgacgcatg
     3001 cagcggtggt ctttgacgcc aagggaaaaa cctttcgtga tgaactgttt gaacattaca
     3061 aatcacatcg cccgccaatg ccggacgatc tgcgtgcaca aatcgaaccc ttgcacgcga
     3121 tggttaaagc gatgggactg ccgctgctgg cggtttctgg cgtagaagcg gacgacgtta
     3181 tcggtactct ggcgcgcgaa gccgaaaaag ccgggcgtcc ggtgctgatc agcactggcg
     3241 ataaagatat ggcgcagctg gtgacgccaa atattacgct tatcaatacc atgacgaata
     3301 ccatcctcgg accggaagag gtggtgaata agtacggcgt gccgccagaa ctgatcatcg
     3361 atttcctggc gctgatgggt gactcctctg ataacattcc tggcgtaccg ggcgtcggtg
     3421 aaaaaaccgc gcaggcattg ctgcaaggtc ttggcggact ggatacgctg tatgccgagc
     3481 cagaaaaaat tgctgggttg agcttccgtg gcgcgaaaac aatggcagcg aagctcgagc
     3541 aaaacaaaga agttgcttat ctctcatacc agctggcgac gattaaaacc gacgttgaac
     3601 tggagctgac ctgtgaacaa ctggaagtgc agcaaccggc agcggaagag ttgttggggc
     3661 tgttcaaaaa gtatgagttc aaacgctgga ctgctgatgt cgaagcgggc aaatggttac
     3721 aggccaaagg ggcaaaacca gccgcgaagc cacaggaaac cagtgttgca gacgaagcgc
     3781 cagaagtgac ggcaacggtg atttcttatg acaactacgt caccatcctt gatgaagaaa
     3841 cactgaaagc gtggattgcg aagctggaaa aagcgccggt atttgcattt gataccgaaa
     3901 ccgacagcct tgataacatc tctgctaacc tggtcgggct ttcttttgct atcgagccag
     3961 gcgtagcggc atatattccg gttgctcatg attatcttga cgcgcccgat caaatctctc
     4021 gcgagcgtgc actcgagttg ctaaaaccgc tgctggaaga tgaaaaggcg ctgaaggtcg
     4081 ggcaaaacct gaaatacgat cgcggtattc tggcgaatta cggcattgag ctgcgtggga
     4141 ttgcgtttga taccatgctg gagtcctaca ttctcaatag cgttgccggg cgtcacgata
     4201 tggacagcct cgcggaacgt tggttgaagc acaaaaccat cacttttgaa gagattgcgg
     4261 gtaaaggcaa aaatcaactg acctttaacc agattgccct cgaagaggcc ggacgttacg
     4321 ccgccgaaga tgctgatgtc accttgcagt tgcatctgaa aatgtggcca gatctgcaaa
     4381 aacacaaagg gccgttgaac gtcttcgaga atatcgaaat gccgctggtg ccggtgcttt
     4441 cacgcattga acgtaacggt gtgaagatcg atccgaaagt gctgcacaat cattctgaag
     4501 agctcacctt gcgtctggct gagctggaaa agaaagcgca tgaaattgca ggtgaagaat
     4561 ttaacctttc ttccaccaag cagttgcaaa ccattctctt tgaaaaacag ggtattaaac
     4621 cgctgaagaa aacgccgggt ggcgcgccgt caacgtcgga agaggtactg gaagaactgg
     4681 cgctggacta tccgttgcca aaagtgattc tggagtatcg tggtctggcg aagctgaaat
     4741 cgacctacac cgacaagctg ccgctgatga tcaacccgaa aaccgggcgt gtgcatacct
     4801 cttatcacca ggcagtaact gcaacgggac gtttatcgtc aaccgatcct aacctgcaaa
     4861 acattccggt gcgtaacgaa gaaggtcgtc gtatccgcca ggcgtttatt gcgccagagg
     4921 attatgtgat tgtctcagcg gactactcgc agattgaact gcgcattatg gcgcatcttt
     4981 cgcgtgacaa aggcttgctg accgcattcg cggaaggaaa agatatccac cgggcaacgg
     5041 cggcagaagt gtttggtttg ccactggaaa ccgtcaccag cgagcaacgc cgtagcgcga
     5101 aagcgatcaa ctttggtctg atttatggca tgagtgcttt cggtctggcg cggcaattga
     5161 acattccacg taaagaagcg cagaagtaca tggaccttta cttcgaacgc taccctggcg
     5221 tgctggagta tatggaacgc acccgtgctc aggcgaaaga gcagggctac gttgaaacgc
     5281 tggacggacg ccgtctgtat ctgccggata tcaaatccag caatggtgct cgtcgtgcag
     5341 cggctgaacg tgcagccatt aacgcgccaa tgcagggaac cgccgccgac attatcaaac
     5401 gggcgatgat tgccgttgat gcgtggttac aggctgagca accgcgtgta cgtatgatca
     5461 tgcaggtaca cgatgaactg gtatttgaag ttcataaaga tgatgttgat gccgtcgcga
     5521 agcagattca tcaactgatg gaaaactgta cccgtctgga tgtgccgttg ctggtggaag
     5581 tggggagtgg cgaaaactgg gatcaggcgc actaagattc gcctgaacat gccctttttt
     5641 cgtaagtaag caacataagc tgtcacgttt tgtgatggct attagaaatt cctatgcaac
     5701 aactgaaaaa aaattacaaa aagtgctttc tgaactgaac aaaaaagagt aaagttagtc
     5761 gcgtagggta cagaggtaag atgttctatc tttcagacct tttacttcac gtaatcggat
     5821 ttggctgaat attttagccg ccccagtcag taatgactgg ggcgtttttt attgggcgaa
     5881 agaaaagatc cgtaatgcct gatgcgctat gtttatcagg ccaacggtag aattgtaatc
     5941 tattgaattt acgggccgga tacgccacat ccggcacaag cattaaggca agaaaattat
     6001 tcgccgtcct gcgtttcttc tacaggctgc atctcgctaa accaggtatc cagtttctgc
     6061 cgcagcttgt ccacgccttg tttcttcaac gaagaaaacg tttcaacctg cacatcaccg
     6121 ttaaacgcca gtacagcttc acgcaccata ttcaattgcg ctttacgtgc gccgcttgcc
     6181 agtttgtccg ctttggtcag cagcaccaga acggcgatat tgctgtctac cgcccactca
     6241 atcatctgct gatccaaatc tttcagcgga tggcgaatat ccattagcac caccagacct
     6301 tgcaggctct gacgtttttc gaggtattcg ccgagcgcac gctgccattt gcgcttcatc
     6361 tcttccggga cttccgcata accgtaccca ggcaagtcaa ccagacgctt gccgtcagcc
     6421 acttcaaaca ggttgataag ctgggtgcgc cctggggttt ttgaggtacg agccaggctt
     6481 ttctggttag tcagcgtgtt cagcgcgctg gatttacctg cgttggaacg gcctgcaaaa
     6541 gccacttcaa ttccggtatc ggaaggtagg tggcgaatat caggcgcact catcacaaaa
     6601 tgcgtctgtt gataattcaa attagtcaaa gcggtcgtct ccgtcagtca aagctttgtg
     6661 gcgattatac ctgaaccata ataaaaggcg gatttttcgg cgtgagcgtt gtaagtaaaa
     6721 gccatacgct ttgtgagaca ttgccgatag tctttatgcg aaatagcaga gaaaattctg
     6781 cgatgcat
//
LOCUS       DAZFWP020000220         6774 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFWP020000220 DAZFWP020000000
VERSION     DAZFWP020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.221"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..214
                     /locus_tag="ACHK0Z_RS23130"
                     /old_locus_tag="pgaptmp_004626"
     CDS             <1..214
                     /locus_tag="ACHK0Z_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504506.1"
                     /translation="KKTKRQGNTRVNIKNAPESFLARCITMHESDARSVSCVLNTERL
                     KTTSFLPLDGKFCTGILDPISHMEFL"
     gene            complement(281..424)
                     /locus_tag="ACHK0Z_RS23135"
                     /old_locus_tag="pgaptmp_004627"
     CDS             complement(281..424)
                     /locus_tag="ACHK0Z_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HGU4504507.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(522..851)
                     /locus_tag="ACHK0Z_RS23140"
                     /old_locus_tag="pgaptmp_004628"
     CDS             complement(522..851)
                     /locus_tag="ACHK0Z_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HGU4504508.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKK
                     GLWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTA
                     FIITLKM"
     gene            complement(841..2511)
                     /locus_tag="ACHK0Z_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     CDS             complement(841..2511)
                     /locus_tag="ACHK0Z_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HGU4504509.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2744..4963)
                     /gene="mobQ"
                     /locus_tag="ACHK0Z_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     CDS             complement(2744..4963)
                     /gene="mobQ"
                     /locus_tag="ACHK0Z_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HGU4504510.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPLASPLPSAHRVDITADAAPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLMVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            5142..5417
                     /locus_tag="ACHK0Z_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             5142..5417
                     /locus_tag="ACHK0Z_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HGU4504511.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
     gene            complement(5633..6526)
                     /locus_tag="ACHK0Z_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             complement(5633..6526)
                     /locus_tag="ACHK0Z_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4504512.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWE
                     ALGISRATYYRKLKCDPDLAK"
BASE COUNT         1594 a         1875 c         1562 g         1743 t
ORIGIN      
        1 aaaaaaaaca aaaagacaag gaaacaccag ggtaaacata aaaaatgcgc cagaatcgtt
       61 tttagcgcgt tgcataacta tgcacgaaag cgatgcacga tcggtttcgt gcgttctgaa
      121 caccgaacgg ctcaaaacga caagttttct ccccctggat ggaaagttct gcacggggat
      181 tttagatccc atctcccaca tggaattttt gtgacacttc aaaaaaaagc gcgtcagaat
      241 cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg ttactgcgtt gctaatccgg
      301 tcagttcagc tgaggatgac ggggatacag tcccgccctg aacatcgcgg atatagtttg
      361 cctgacatgc agcaagaata atggctgcaa gaagcaataa aataatccat gttatttttt
      421 tcatactgtt ccttgtatat atggactcag gcattcatcc ataaaaacct gaaatccaaa
      481 ggttgaaatc atccatgaaa cataaaattc tataacacta tttacatctt taacgtgata
      541 atgaaagcag tgtatccatt ctcaatatca tctttaatat tttcaatagc caatctatta
      601 ttttcaggga tttttgattt gtattcctcc ggcatatcat atgtccatga tgctgaacta
      661 tctatcctga atccaggatt cagccaatgc ccaccatcaa tgcccaaaat ccataagcct
      721 tttttcgctg cttcattaca aacttctatt gcagcttttg gtgataactt catagaagca
      781 tttcctttta attcaaaaaa ggcatcacca ctatcatata acaccgtatg ttcaaataat
      841 ttattggtca tttttatttc cccattgaat tctcgaatcg tccacccaac ccggatctgt
      901 cttatcacta atctgagtaa cctcaccagt acgatcattg acaacaacat atttaccagg
      961 agaaccatac actgttgctg gatcatttcg acctaaatag tctggaggtg ttttcttcgg
     1021 actacgctta tcaaaagatg taccagttgc accgttagat acggtatttt tgatatcatc
     1081 ctctgtccat cctctttctg gcatttgtcc cctaattttc tgatctattt ttagacctgg
     1141 tatctttcct ttattttttg ctaactcatc atttagtttg ccttcagcat ctcttttatt
     1201 atcctctttc ttcttcctgc tttccatcgc agtacttaat gcagcatcag catctgcctt
     1261 ctcttttgca gcagcatcaa atgcagcctg cttattattt acatccgtct gcgcccgctg
     1321 agctttaagc ccggccattt gccacattct gtgaccgcca gccattgggt catgtgcaaa
     1381 tcgttcaaat tgttttattt cagctattgc atcagcaaga gttttattcg ctgcatcaag
     1441 ttcgctttta cgcgaattat aaacctgaac agctttagcc tgtcgctcct gatttctggc
     1501 aacatcttca tttgcctgat tcagctctgc acgcgcgcgt tcataatttc gctcagccgc
     1561 ttcaaccgga tgcgtagcat cccattcctg ctgacggcga ttttcttcat cttgacgttg
     1621 ttttacctgg tcagggctaa gaacatcact cactgaaaca tatacggcat tatgaccgct
     1681 gtccttcggg aatcgaataa ctgcatccct ggtattaccc ccctgagtaa atcctgccgg
     1741 gcgaacatcc ttatcagtat tatttgtaac acctgggctt aatgtctgta ctgctggcgt
     1801 actgttatta actgaaatat tcagaacagg tgcacctgga attgatgccg taaaaacacc
     1861 tggacgttcg gtaggttttg catcaaccac cggaacactc atcggaacac ctgaaacaac
     1921 cgaaatattc tgtcgttcgt cttttacatc atcaacaaca cgaacattta cgtttactgt
     1981 tgccttatcg agaggtaatg aactgacagg tgattcagta atatcatctg cgggtaatga
     2041 cgtcacaatc tttgacatca tattggggtc atctttcgct atttgtgatg gcaatacacc
     2101 atataaagcc accccccaaa gaccaaattt aaacggtcct ttcagggcag ccataatatc
     2161 agcaatagct gccgataatg ctcccgctga aatactgacc gccagaccgc cagctcctgg
     2221 agtggaaagt gccggaaaac caaatgccac tggcgcagct actgctgaca gattaccgcc
     2281 tgttcccgaa ccaccaccgg aattaccatt ccccccgcca ttaccatgac cggaaccacc
     2341 accccagtga atgccgctac cggaaccacc accccacggg ttattttccg aactccatcc
     2401 ggagccatca gaagcaccac cacctacacc aagcccggtc gggccaccat taatgttacc
     2461 acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg ccaccgctca taaaaattcc
     2521 ctcttttaag cgttaaaaca attaattaca tgattaatac tgtacataaa accactggtt
     2581 ttatgtacag atcaaaaagt acccgtcatc gttttttctg tcaagttcag gtggaaaatt
     2641 ttttagaaat caggagggat aacacttaga aaacgcagag cgggccacaa aaaattttgt
     2701 ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg atatcacata ctcatgcccc
     2761 ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc gtcccgcttc cactcatgcc
     2821 aggtctgatt aaaaccgagt tgacggaaga gataatccgg catctcttcc ggtttgtgga
     2881 cagcagccaa aagcctgtca tgatggcctt tgagccggaa ctgcatcagg tgcggttccg
     2941 gtgggtacgg agtatcctca tcatggttta tagcttctcc gtaacctgcc aggcataccc
     3001 cgttctccgt gatggtgctg accatcagat gactgtaatc cggcgacgga agcacaagaa
     3061 cggggcaaaa ccctccttcc cggcgaacaa acccgacaag catttcctcc ggccctttgc
     3121 acaacaacag attcagcgtc ctgctgtttc cctctctcga aaatacaaca ggcatgagtt
     3181 gttccggtgc cgcatcagcg gtgatatcca cccggtgcgc tgacggcagc ggagaggcta
     3241 agggcctacg gtcgtcgctg aaataaaaat tatccggcca gtgatgcggc agcgccggaa
     3301 aaccgtaaga gggccacccc tggcgcatct gttccagtac atgccggacc gtcgcaccgg
     3361 aatacattct cacacgctca ggccgcgcca tcgtctcatg agattctggc attccctcac
     3421 gtatatccga gaccatcaca gccccgcctg gcgacaggta aacattcacc gggtcatgaa
     3481 cactctgggg gtaatgcgcc agctcatcac cccataccgt gaattctccg gctctgttac
     3541 tcagcatcat gtagcgggtt atcctgcccg ttcccggctc tctggcacgc cccagctccg
     3601 taataacccc gactaatgcc gtttcacggc tcagacggtg gttatcatga cgtttcagcc
     3661 gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc ggcttcctcc ctgcgccgct
     3721 ccgcttcccg tgctgacagt ttttctttca gctctgccat tcccggcgca atccacgtat
     3781 tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc ccgttctttc acggccctgt
     3841 aggtgcggcg aagagattca atctgtttcc cgagtttcca gaaacggagt tgtcttttgc
     3901 ggtattccgg ttcagcccat gtcatcaggg gaatctctga cagaagggac gcgacagggc
     3961 gtgctttttt gagtccggtc atctcctttt cgagaatggc ctgctctttc agcagcgcat
     4021 tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc caggaactcc ggggtcatct
     4081 cccacgcgtt cccgcattcc tgcggcagcg tggcaatgaa ttcccgcagg gtatccgctg
     4141 aggcattcgc agcgggaatg gtcaggttaa gggcttccgc tgcacgacgt gcggcatcgc
     4201 tgagttcccg tttccgcacg ttctcctgta cctggatgac tgccagcctt gtgcgggaaa
     4261 cctgtatttc caggttaagg ctgttaatca gacggttctg ctcaccgcgt tctgtttcca
     4321 ttccccgttt ttccatcgca caggcagcct ttccgaggtg aaggccgggc gctttctcca
     4381 gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg gtagccggcg ttcgcaagcg
     4441 ctctgttcgc atggtcagcc catgatgcgc gccacgtctc ggcgagtgcc cggtcgttcc
     4501 agtccctgac ctttcctgcg aatcccgttt ccccgacagc tctcgtggtc agcatgatgt
     4561 gcgcatgggg attggtccgg tccatgtgat ggaacgccac atcggcaatc atgccccgac
     4621 tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg tgcggcctcg cgggaaatct
     4681 cacggggaat ggcaagttca atctcccttg ccagctggga gttccggcgc tgttcggctt
     4741 tctcgaccgc attccagaga acggaacggt caccacacca ggacggtgca ttcgcagggg
     4801 tgagaatgac cgcatcatca acgccgcgtt tacgggtgta atcgtggatt aatccggtac
     4861 ggtcatcggg tatgacagag ccggaacggt aggcagcaga agcaacagca ctgtagccgt
     4921 tttttcgcga aatgattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     4981 tttgccgttt tcttttagcg tcgcagaccg cacacaatcg aaacgaagtg gagattgtat
     5041 aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc ggttttgcaa ctaccagtga
     5101 cgcgctaaaa tgcgattttt caccgcgcac ggagtcacaa aatgagcaga acactcgaac
     5161 agaagattgc cgaagctgag gccagattac agcgcctgaa ggcgaagagc cggagcctgg
     5221 acacggcgca aaaagtcatt gtgggggctg ccatgctggc gagggtacgg aggcctgaag
     5281 aagcgcaact gcgtgcgttc ctgctccagt ttctgcgaaa ggaagtcaca agacaggcgg
     5341 atgtgaaccg actacaaccg ctgattaacg agctggagaa gctacccaga ccaccggcga
     5401 aaccgcagaa ccactaagcc ccggcgcgta agtgcccatg caccaccggg cgcctttgcg
     5461 cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagcg ctaccacctc
     5521 aatcatgcgg aaagccacgc cattacgggg cagaacggag gaaatcgcaa gaaagatcac
     5581 tgctcaaaaa gacgccaaaa gggcgctgtt atctgataag gcttatctgg tctcattttg
     5641 cgagatctgg atcacatttt aattttcggt agtacgtcgc tcgactgatc cctaatgcct
     5701 cccacggctt cagcgaacgt gctgatgtag gaactgctgc gcgcttagat ttagttccac
     5761 ccttgcgacc tcgagcggcc tgtacagcgg aaaatccctc tggtgagaat ttcctgtgtg
     5821 tatatttcgc aatgctcttg ccaatagccc gacattcagc cggagaaagc ggaacgggaa
     5881 gcgatgcgtt gtacatttcg acacgctgga tcacggcatc aagccattgt gagaatacag
     5941 gccagccctg acgaatcgct ctgtaggccc atttacggac cttttcgaac agatggtagt
     6001 ttcgccccag cccgtaattt ttatcgacgc tacggcgcgc tgaggcgctc aaatcgagat
     6061 aatcagccag ttcgtcgaga gtgtagggtt cctcgcgcca ttccacttcc tgccattcag
     6121 ggtggcacgg atttttgcag atcaggccgc tgtaattcac atccgcgccc agtttttcac
     6181 acaacgcacg ctcaatagcg gcagcgtatc tgagcgccga agccgatgca tccggcgcag
     6241 ttctcacagg aagggcgagc gcgtagagca gatgcgcgtg cccgttacgg ggatttttta
     6301 cggtgatgtt aggggccgga caattccggt cgctccagtc gattgatgcc cctgtccggt
     6361 ccacgtcaaa gggaagccag tacagtgcat gtggctggtt ctgctggatg aaacgggcaa
     6421 gaatggcaat gtttttcggc agaatgcgaa cgccgtacgc gaaatcgttc gtacagtacg
     6481 gtttgtgcgg taatttttcc ctgaagcgtt gaagtacggc gctcatgtga tacgatttct
     6541 ctcggtacac agtttaacta accgcgcttt cccatcggga aacgcggaac ccggcgccct
     6601 gtaatttcgt gatcaatctt ggcggtgaga gcacgattta cggggcgttt gtttttcagt
     6661 cgcttacact acacccaacg gcaaatcctg tcgatccttc ccgaaaaacc gatccattta
     6721 ccataactcc cgttgtacgt accccttaaa acaccctcaa tcgctttctg acgc
//
LOCUS       DAZFWP020000221         6750 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFWP020000221 DAZFWP020000000
VERSION     DAZFWP020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.222"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(193..1122)
                     /gene="fdhE"
                     /locus_tag="ACHK0Z_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             complement(193..1122)
                     /gene="fdhE"
                     /locus_tag="ACHK0Z_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU4504513.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1119..1754)
                     /gene="fdoI"
                     /locus_tag="ACHK0Z_RS23170"
                     /old_locus_tag="pgaptmp_004634"
     CDS             complement(1119..1754)
                     /gene="fdoI"
                     /locus_tag="ACHK0Z_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4504514.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1751..2653)
                     /gene="fdoH"
                     /locus_tag="ACHK0Z_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             complement(1751..2653)
                     /gene="fdoH"
                     /locus_tag="ACHK0Z_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU4504515.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2666..5716)
                     /gene="fdnG"
                     /locus_tag="ACHK0Z_RS23180"
                     /old_locus_tag="pgaptmp_004636"
     CDS             complement(2666..5716)
                     /gene="fdnG"
                     /locus_tag="ACHK0Z_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5129..5131),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4504516.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5910..6743
                     /gene="fdhD"
                     /locus_tag="ACHK0Z_RS23185"
                     /old_locus_tag="pgaptmp_004637"
     CDS             5910..6743
                     /gene="fdhD"
                     /locus_tag="ACHK0Z_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU4504517.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1468 a         1837 c         1837 g         1608 t
ORIGIN      
        1 agcgcatcag gcaatcttac gcttgtcatc agtgacatac caataacctt atcgatgtgt
       61 tggagggaag aacgaggcaa acaggcactg gcggcatagc ttgaggtggt aacgcggaat
      121 acgggagatg gtgtcgtcac accctatggg ctgtaatccg ggcgacctgc gccacccgga
      181 aaacgtcaga gattactccc cttcacccgg gaacaggaac gggttgatgg aactgcgggc
      241 gtagccttct tgctccattc gcgcgtccag taccagagag gcgaggtcat ctgccacggc
      301 ttcaactttc ggttcttttt cctgataaag aatcttcagg taagtgccgc agtcatcgca
      361 gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca gtttgccgct
      421 ctgttcacag ttgctgcatt ttacgcgcac tacgtgccat tcggtttcac acaggttgca
      481 gtgcaggtaa cgcagaccct gagtggtgcc aatttgcacc atgctggaca ccggcataga
      541 accacaaacc gggcaatatt gacgttgctc gccgtattca gcgcgggctt tgccggggat
      601 cagattggcc atctgcgccc agtagagcga cagtgcagcc cagataaacg gcgctttatc
      661 gctgctgacg gatgagaaat cagaggcaaa cagtgcgctg gccatatctt ccagctcctg
      721 agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca tttcaggttt
      781 cagctcagca atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg
      841 aatatccagc gggggcttgc cctgtgcgct ggcttcttta atgcgcgcgg tcagatccat
      901 ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg
      961 caggtaatca cccagcggat tattttctgc cagctcgcgc agacgctcgg cgcggcggtt
     1021 gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg tacgtttctc
     1081 gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt cttttccgtt
     1141 gtcttgcgga cctcacggta ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc
     1201 catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc
     1261 atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc gaatcggatc
     1321 accgggattg agaaagcagg cgcaaaataa ggacgccaga taatcacgcc gctcaccagc
     1381 agcagaacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa gttataacgc
     1441 ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc ccaaaagata
     1501 tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat gaacgaggca
     1561 aacataacca cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt gcccatgatt
     1621 tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc cgccaggatg
     1681 aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg cacgatggtg
     1741 tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc tcttcctcat
     1801 ccgcacggtt cggaccgaca cccacgtagt ggaagatact ggccgcaaag gtagccgcaa
     1861 agccaacagc cgcgagcggt ttccagatgc ctttccagaa tttcacggtt tcgctgattt
     1921 ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc agcacgtaca
     1981 tgacgtgtgt accaccgacg cctgccggat cgtacagacc cgcattgtcg taaccgcggg
     2041 ttttcagctc agcaacgcgc tcgctcgcca gcgttttcat cgactctttc gtaccgaagt
     2101 gaatcgcgcc cgttgggcag gtcttcacgc aggctggttc ttgcccaacc accacgcggt
     2161 caacgcacag cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga
     2221 acggacagcc cgcaatgcaa taaccgcagc caatgcactg ctcggactgg aagtcgacga
     2281 taccgttggc atactgaata attgcacctt ccgccgggca cgctttcagg cagcctggat
     2341 cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg ttctgctcca
     2401 cttccgagaa gcgcattacc gtccacgatt tggcgcttaa atcattgggg ttgtcgtaca
     2461 ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac
     2521 aggctttaca gccgatacag gtggtgacgt cgatgagttt cgccacttct tcctggaagt
     2581 cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg atatcttgag
     2641 attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg aaggacttaa
     2701 actccggcgt ctgcgtgttc gcatcaccaa cgaatggcgt taacgtattg gcaataaagc
     2761 cttttttagc tacgccttca taaccccagt gaatcggaat accgatggta tcgatatctt
     2821 tgccgttcgc tttcagcgta cgaatacgtt tggtcaccac cgctttggct ttgatatagc
     2881 cacggttgga ggagactttc acggtatcgc cctgggcaat gccaagttta ttcgccagcg
     2941 actccccgat ttccacaaac tgctctggtt gcaaaatcgc gttcaacagc gcgtgtttgg
     3001 tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg aacttatcgg
     3061 ctttacccaa tgcttcggcg tcgtctttaa agatacgtgc agccgggttc gagataacgt
     3121 ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg
     3181 gaccttccgc catcttatcg agggcaaaca gacgccccat gccttcctgc tgcatgataa
     3241 acggcccgac gccgctaccc ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc
     3301 acttagtgcc gtcccatttc agcaactgac gcttcggatc ccacgggtta ccctgcggat
     3361 ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat gcccagccca
     3421 gcgtgttacc gaggccagac ggatcggcgt tatcacgacg cgccatctgg ttgccttccg
     3481 gcgtccagct accggcgaaa atccagcagc cacaggaggt tgtaccgtca tcgcgcaatt
     3541 gggcgaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt gccggatcgg
     3601 taatatcggc cagcgcctta ccgttgctct ccatcgctac ttcttccgac ttcggctcat
     3661 gcggaatggc gtagttccag gtcatgttca gcacctggtc cgggttcgcg ccaccctgtt
     3721 cggcatacat cttgcgcaag cgcaggaaga taccggagag gatctcgcca tcagtcagcg
     3781 caatccccgg ggcgtccgca cctttccagt gccactgcaa ccagcgacct gagttaacga
     3841 ttgaaccgtt ctcttctgcg aagcaggtcg atggcagacg gaacacttcg gtctggatct
     3901 tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag gtttcagtgt
     3961 tcagtgggtc gatggtgacg aggaacttca gtttcgacag acaaccgatc actttgtttt
     4021 tgttcgggaa tgaggcaaca gggttaaagc cctggcagat atagccattg accttgccct
     4081 ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt atcccacttc ggcaaccagt
     4141 caaagcccca gctattttcc gccgtcgctt tatcaccaaa gaaggccttc atcatagaga
     4201 cgaagaattt cgggtagttg ccccagtagt ttacctggcc ttccagcagc ggttttggcg
     4261 tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc gcttggcagc gtcatgtaac
     4321 ctggcaggct ctgcgacagc agccccaggt ccgtcagccc ctgaatattg gagtgaccgc
     4381 gcagggcgtt aacgccgccg ccggccatcc ccatattgcc gagcagcagc tggatcatcg
     4441 ccatcgtacg aatgttttgc gcaccaacgg agtgttgcgt ccagccgagg gcatacagga
     4501 acgaggcagt tttatcgtga gcactggttt ctgcgatgta ttcgcagact ttcaggaacg
     4561 cgtcttttgg cgtaccacag atgttttcaa ccacatctgg cgtgtagcgg gaaacgtgct
     4621 gtttcagcaa gttccacacg cagcgcggat gttgcagcgt ggtatcgcgt ttggcgaagc
     4681 cgttttcgtc cagttcataa gtccaggagg atttatcgta cttgcgtttt tccgcgtcgt
     4741 agccggtgaa caggccatct tcaaagccgt aatcctcacg cactatcagg ctggcgttgg
     4801 tataggcttc ggtgtattcg cggttgaatt tttcattgtt cagcaggtac agcaatacgc
     4861 ctgacaggaa agcaatgtca gtaccggaac gaataggggc atagtagtca gctaccgccg
     4921 ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccgttgtga attttggctt
     4981 ccatcgccca gcggaacccg accgggtgag cttcagcggc gttaccgccc atcaccacga
     5041 cgaggttggc gttcttgatg tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg
     5101 gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca
     5161 tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc gacgcggaag
     5221 cacacagcat cccggtggag agccagcggt taacagtcac gccttcggcg ttttgcgcaa
     5281 tgtagttagc atcgcggtct tctttcatca gtttggcgat gcgatcaaac gcctcttccc
     5341 aactgatttg ctgccattta tcagaacctg gcgcacggta ttccggaaac ttcagacggc
     5401 tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgca ccgcggttga
     5461 ccgggtgatc cggatcgcct tcgatatgga agatagatgc tttggcgttt tttgctccgt
     5521 caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag gtattacggg
     5581 tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagcgctacg ctgggtgcaa
     5641 aacccagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta aagaactgcc
     5701 ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca tttacattcg
     5761 cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc
     5821 agaattggct caatttccct ccatccagga agggtttgta acagaatacc ataatgttgg
     5881 tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcaaaaa gaaatcgaaa
     5941 atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg gcggcgtgat gatttacaac
     6001 acccacggtt agatgaggtc gcggaagaag ttcccgttgc gctggtctac aacggcattt
     6061 cgcatgtggt gatgatggcg tcgcccaaag accttgagta ctttgcgctc ggtttttcgc
     6121 tttccgaggg gataatagaa agtccgcgcg atatcttcgg catggatgtc gttccttcct
     6181 gtaatggcct tgaagtacaa attgagcttt ccagccgccg ctttatgggg ttgaaggagc
     6241 gccgccgggc gctggcggga cgtacgggct gtggcgtatg cggcgtggag caacttaatg
     6301 acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc aacaaactgg
     6361 atgatgcatt acgtcatctc aacgattttc agccagtggg gcaactgact ggttgtactc
     6421 acgccgctgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc
     6481 gccatgtggc gctggacaaa ctgttaggcc gccggtcaca agaaggggaa agctggcagc
     6541 aaggtgcggt actggtttcc agtcgtgcca gttatgaaat ggtgcaaaag tcggcgatgt
     6601 gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac gcttgctgta gaagtggctg
     6661 agcgctgtaa tctgacactg gtaggttttt gtaaaccggg tcgggcaacg gtttataccc
     6721 atccgcagcg tttgagcaat taattaaaat
//
LOCUS       DAZFWP020000222         6505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFWP020000222 DAZFWP020000000
VERSION     DAZFWP020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.223"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1520
                     /locus_tag="ACHK0Z_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /pseudo
     CDS             1..1520
                     /locus_tag="ACHK0Z_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1489..3225)
                     /gene="aceK"
                     /locus_tag="ACHK0Z_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     CDS             complement(1489..3225)
                     /gene="aceK"
                     /locus_tag="ACHK0Z_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU4504518.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3506..4810)
                     /gene="aceA"
                     /locus_tag="ACHK0Z_RS23200"
                     /old_locus_tag="pgaptmp_004640"
     CDS             complement(3506..4810)
                     /gene="aceA"
                     /locus_tag="ACHK0Z_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU4504519.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4840..6441)
                     /gene="aceB"
                     /locus_tag="ACHK0Z_RS23205"
                     /old_locus_tag="pgaptmp_004641"
     CDS             complement(4840..6441)
                     /gene="aceB"
                     /locus_tag="ACHK0Z_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU4504520.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLASCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1661 a         1657 c         1568 g         1619 t
ORIGIN      
        1 cagacaaatg aacaccaaac cctgagtgaa ttgttttata aagaactcga ggataaattt
       61 tctggcaaag agctggcgac gcctctatta aaaagcttct cagagaactg tagacaaaat
      121 ggtcgtcata tctttagtaa caaggatttt gtcattaaat tttccacgtc cgtcttacaa
      181 gctgataaga aagaaattac gataattaat aaaaacgaaa acacgacact tactcaaacc
      241 attgccccaa tatttgaaga atacctaatg gaaattttac ctcaacgctc agacactctt
      301 gataaacaag aattaaacct aaaatcagat agaaaagaaa aagaattccc aagaattaaa
      361 cttaatggtc aatgttattt tccggggcga ccccaaaacc gtatagtatg ccgacacatt
      421 gctgcacaat atattaatga tatttatcag aatgttgatt acaaacccca tcaagatgat
      481 tactcttcag ctgaaaaatt tctcacgcac ttcaacaaaa aatgcaaaaa ccagactttg
      541 gcgttggttt ccagccgtcc tgaggggcgt tgcgttgctg cctgcggtga tttcgggcta
      601 gttatgaaag catattttga caagatggaa tcaaatggca tcagtgttat ggcagccata
      661 ttactggtgg ataaccatgc tttgacggtc cggctaagaa taaagaacac aactgaagga
      721 tgtacccatt acgtggtttc ggtttatgat cctaatgtaa ctaacgataa aataagaatt
      781 atgagcgaaa gcaaagagga tattaaacac tattctctga tggattttat gaatgtagat
      841 tatagcctcc tgaaatggtc aaatgatcat gttattaacc aatctgttgc aataattcca
      901 gcacttccga aagaacagct attgatgtta aaaggatctg tggatgaaat aacccctcca
      961 ttatcaccag caacgatgaa tttgctaatg gcaattggtc agaatcacca acttacgcaa
     1021 ctgatgattc aactccagaa aatgccagaa ctacatagaa cagaaatgtt gactgcctat
     1081 aatagtggac atatgaacgt tattaatact atttttaacg cattacccac tctttttaat
     1141 acgtttaaat tcgataaaaa aatatgaagc ccctcctcct ggcaaataat tctaatgaat
     1201 atcccggttt gttttcagcg atacagcata aacaacaaaa cgttgtagag acggtttatc
     1261 ttgctttatc tgaccatgca cgcctgtttg gatttaccgc tgaagatatt atggattttt
     1321 ggcaacacaa agcgccacaa aaatactctg cctttgagtt ggcttttgaa tttggtcacc
     1381 gggttattgc tgaattaatc cttaatacat taaataagat ggctgaaagc tttggcttta
     1441 cggataaccc tcgatacatt gcggagaaaa attatatgga agctttactc aaaaaagcat
     1501 ctccccatac cgtacgctaa atctttgcct gcgccgatac gcataaacat cttccacatg
     1561 cccttcacgt atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca ggtcggcgtg
     1621 catctcttca aacagcggac caatacgcgg gtcggcgcat agccagtggc gaaactcttc
     1681 cgggaaaaca tcgcccggcg agacgctgta ccacggttcg ctggcaagtt cgtcttccgg
     1741 atagcgcggc ggcgggatgt cgcggaaatt cacttccgtc atgtagcaaa tttcatcgta
     1801 atcataaaaa accacacgcc cgtgacgggt gacaccgaag tttttaaaca gcatgtcgcc
     1861 aggaaaaatg ttagcagcgg caagctggcg aatagcgtta ccgtattctt caatggcgtc
     1921 gcgcaactgc tgaccttcca cttgttccag ccagatattg agcggcacca tccgccgctc
     1981 gatatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt ccgctgcttc
     2041 ctggagcagt aattccatta atgccgggga aatatgccgc ttctccagca caaagttttc
     2101 aaactcctgg gtgtccgcca ttcggcccac gcgatcgtgc tctttcacca gttgatagca
     2161 ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt tgatgacttt
     2221 gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac gaatacccgg
     2281 cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc ggtagctttc
     2341 ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg ttttacctgg
     2401 cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata
     2461 agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat
     2521 aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg tgccggaagg
     2581 cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt cgttcgccac
     2641 ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat
     2701 gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt cgctgataac
     2761 gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca gcgggcgggg
     2821 aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct cgggagtaag
     2881 cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg caatctcgaa
     2941 gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta aaaatgccgc
     3001 gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac caacgtgatg
     3061 atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt cggcctgttc
     3121 gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag cgtcgaagcc
     3181 ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc gccctcatca
     3241 ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt cggcaacggt
     3301 tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc
     3361 ggcgtaaatg ccttatccgg cctacgttcg gcaacggctg taggcatgat aagacgcgca
     3421 agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtgaacgcc ttatccggcc
     3481 tacagtcagc aacggttgtt gttgcttaga actgcgattc ttcagtggag ccggtcagcg
     3541 cggtgactga agacgtgccg ccctgaataa tagtcgtcac tttatcgaag taacctgtac
     3601 ccacttcctg ctggtgagat acgaaggtat agccatcttt cgcggcggca aattccggct
     3661 gctgcacttt ctcaacgtag tgcttcatac cctcgccctg ggcataggcg tttgccaggt
     3721 caaacatgtt gaaccacatg ctgtggatac ctgccagggt gatgaactgg aacttgtagc
     3781 ccatatccga cagctgctgc tggaagctgg caatagtttt gtcgtcgagg tttttctgcc
     3841 agttgaacga cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag
     3901 cttgtgcaaa gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cagaccaggt
     3961 cagcatatgg cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac
     4021 ggaagaagcc ttcactggta cgctcgccag taataaattc actgtcatac gggtcgcaat
     4081 cggaggtaat cagatccgcc gcatcggcat cggtacgagc aaccagcagg gttggaacgc
     4141 ccgtcacgtc agctgcaaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca
     4201 ccaaaacttt gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt
     4261 gaactgccgc tgcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac
     4321 cgccaaaacc ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat
     4381 cgcccggctc aatgcctgcg gaccattgga tctgatcggc acgacggaag gtgttgttga
     4441 tccgctccac cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc
     4501 tggccgccag gttagcgtcc gccgctacct gccatcccga cagatagact gcttcaatac
     4561 ccgctttcgc ctgttgcagc gcctgaccgc cagtcagtgc accgaggctg ttgatgtagc
     4621 cttttttcga ctcgccatgc agcagacgcc acattttcgc cgcgcccagt tgcgccaggg
     4681 tgcattcagg attgactgaa ccgcgtaatt tcaccacatc ttccgcgctg tatgggcgag
     4741 taatgccttc ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac
     4801 gggttttcat gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc
     4861 tggcagggtc aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc
     4921 ggcatcgtca aaacgcccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt
     4981 catctcttca cccagcatct ggcggaacaa ggctttggtc accggtttgc cattgctcaa
     5041 cgttttttga tgatggatcc actgccagat tgaggtacgg gaaatttcag ccgtcgccgc
     5101 atcttccatc aggccataaa tcggcacgca gccgttgccg gagatccatg cttcgatgta
     5161 ctgcactgcc acgcgaatgt tggcgcgcat accttcttcg gtgcgttcac catcacaaga
     5221 tgccagcagc tgatcggcag taatcggcac gtcttgttcg cgcatcactt caagctgatt
     5281 tttacgggag ccgagaatgt cgttgaatac cgccattgcc gtatccgcaa ggcctgggtg
     5341 agcaatccat gtgccatcgt gaccgttatt ggcttccagc gctttatccg cttttacttt
     5401 gttgagcacc tggttattgc gctcttcatc tttgctcgga ataaacgctg ccatgccgcc
     5461 catcgcaaaa gcaccgcgtt tatggcaggt tttaatcaac aggcgtgagt aagcattcag
     5521 gaatggttta tccatcgtca ctgcctgtct gtctggcagg acgcgatcgg gatagttttt
     5581 caacgttttg atatagctga agatgtaatc ccaacgaccg cagttcagac caacaatatg
     5641 gtcacgcagc gcgtgaagga tttcatccat ctggaacacg gcgggcagcg tttcaatcag
     5701 caacgtcgcc ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac
     5761 ttcgctccac caggccgctt cctgccagga ctgggttttc ggcagataga aatagggacc
     5821 gctgcccttt gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag
     5881 gctaccgggg attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg
     5941 tacccgacaa atcaaaaccg ctggattggg cttgagctga taaattttgc ctgcttcatt
     6001 ggtatagctg atggtgccgt taaccgcatc gcgcaggtta atttgcccgt cgatcacttt
     6061 gttccagtct ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt
     6121 cagtgcgttg atcaccatct tgcgctcaac cgggccagtt atctcgactc gacgatcttg
     6181 taagtccgcg ggaataccac gaattttcca gtccgtatcg cgaatggaag ctgtttccga
     6241 aataaaatca ggcaaagttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag
     6301 aagtttattg cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc
     6361 ggcagtaaga atttgcttct cctgctcgcc atacggcctt atgaaagcca gttcatcggt
     6421 tgttgttgcc tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttccccact
     6481 gtgaacgaag gatcgttgtg cgctt
//
LOCUS       DAZFWP020000223         6356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFWP020000223 DAZFWP020000000
VERSION     DAZFWP020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.225"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(62..280)
                     /gene="ydfC"
                     /locus_tag="ACHK0Z_RS23210"
                     /old_locus_tag="pgaptmp_004642"
     CDS             complement(62..280)
                     /gene="ydfC"
                     /locus_tag="ACHK0Z_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4504521.1"
                     /translation="MQKREPVIIAPDYTNDELYEWMHQKINAAQDLKWANEVRAKQAE
                     NLSSLEQDITNLEKAAALTIARMITYPR"
     gene            complement(310..438)
                     /locus_tag="ACHK0Z_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             complement(310..438)
                     /locus_tag="ACHK0Z_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504522.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEAHSFNEFKQVLSSSAKSNG"
     gene            complement(440..595)
                     /locus_tag="ACHK0Z_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             complement(440..595)
                     /locus_tag="ACHK0Z_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4504523.1"
                     /translation="MDTIDLGNSESLVCGVFPNQYGTFTAMTYTKSKTFKTESGARRW
                     LERNSGG"
     gene            complement(914..1390)
                     /gene="racR"
                     /locus_tag="ACHK0Z_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /gene_synonym="rarC"
     CDS             complement(914..1390)
                     /gene="racR"
                     /locus_tag="ACHK0Z_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HGU4504524.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFILSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1515..1838
                     /locus_tag="ACHK0Z_RS23230"
                     /old_locus_tag="pgaptmp_004646"
     CDS             1515..1838
                     /locus_tag="ACHK0Z_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4504525.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            1822..2247
                     /locus_tag="ACHK0Z_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             1822..2247
                     /locus_tag="ACHK0Z_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4504526.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            2270..3232
                     /locus_tag="ACHK0Z_RS23240"
                     /old_locus_tag="pgaptmp_004648"
     CDS             2270..3232
                     /locus_tag="ACHK0Z_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4504527.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            3239..3979
                     /locus_tag="ACHK0Z_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             3239..3979
                     /locus_tag="ACHK0Z_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4504528.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            4005..4775
                     /locus_tag="ACHK0Z_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             4005..4775
                     /locus_tag="ACHK0Z_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4504529.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLLSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            4791..5186
                     /locus_tag="ACHK0Z_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     CDS             4791..5186
                     /locus_tag="ACHK0Z_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4504530.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            5243..5827
                     /locus_tag="ACHK0Z_RS23260"
                     /old_locus_tag="pgaptmp_004652"
     CDS             5243..5827
                     /locus_tag="ACHK0Z_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4504531.1"
                     /translation="MITFTKEQLISHVSENVKAMKFAVKQTVFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     NKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            5943..6047
                     /locus_tag="ACHK0Z_RS23265"
                     /old_locus_tag="pgaptmp_004653"
     CDS             5943..6047
                     /locus_tag="ACHK0Z_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504532.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            6293..>6356
                     /locus_tag="ACHK0Z_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             6293..>6356
                     /locus_tag="ACHK0Z_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein hokC"
                     /protein_id="HGU4504533.1"
                     /translation="MKQQKAMLIALIVICITVIVT"
BASE COUNT         1634 a         1380 c         1746 g         1596 t
ORIGIN      
        1 gcactccacc tcacccgtgg agaactcctt aattaccaac cttagcttcg ttggttagct
       61 attaacgcgg gtatgtaatc attctggcaa tggttaatgc cgctgctttt tccagattgg
      121 tgatatcctg ctccagagag gacagatttt cagcctgctt agccctgact tcattagccc
      181 atttcagatc ctgcgctgca ttaattttct ggtgcatcca ctcataaagt tcatcattgg
      241 tatagtctgg cgcgatgatg acgggttctc gtttctgcat actgattcct cgcggtgctg
      301 tttcgcttat cagccattag attttgccga actggaaagc acctgtttaa attcgttgaa
      361 gctgtgagct tcttcgcctt cggcaaggcc ttcgaagtat tcttcgtaag ccttttccat
      421 gattgtgtcg aaatccatat cacccacctg aatttctttc cagccagcga cgcgctccag
      481 attcggtttt aaacgttttg cttttggtat acgtcattgc ggtgaacgta ccgtactggt
      541 tggggaacac gccacatacc agagattcgc tgttgccaag atcgatagta tccatgctga
      601 cctcatttcc ccttaacgcc ggggtagcgg aacaaaaacc tgctgcatag ttattaaagt
      661 tgaaccctgc cgtcatgttc ttacgcctcg ggctggctac ttaacccgtg accactgcct
      721 ggtaactcga ggtattgccc tgtattgtgt gggacgggat gggttggtat gggaaaacta
      781 taggaaatgc ctaattactt gtcaataggc tatgcctaat aatttgggcg caacctaata
      841 ggtgatggtt tgtggaagag gtagtaggag ttaactaacg ggaactagga atttcccgtc
      901 ggaccatata agtttaagtt cctgtcttgg tgatgttctg gcttttccgt tttgattctt
      961 gatttttcag atagttagct accttcattt ccattgcggc aatgtaggcg cgaacgtcat
     1021 gatcaaccca actaggctcc gtagcatttc cagataagat gaaagccaca attgctcttt
     1081 tttcatcaga ggcggcttga taaaggctgt ttatgtctaa aagttcactt tttgtatctg
     1141 aagtggatgg ggttggtatg gggtattcgt taagccccca atgctctgga ccaaccacat
     1201 cagaaaagaa acgccatagt tctggaagtt tgtctttact tatcgaacct ttcttaatcc
     1261 agtcatggat tgatggtggt tggactttga aatgacgtgc gatttccgcc tttgatttga
     1321 cggctcctga tgcaattttt ttgttaatgg cctgctctat cgctcggcct aagtctttac
     1381 cactaagcat tgcttaatag tctcctatgc gcatcgcgtt aggcaatccc tactctcgat
     1441 gtattaggca tagcctattg acaatttcat taggctaagc ctaatattgt tgcgtgtttt
     1501 ttggagttca ttcgatgaaa aaagataact attcattcaa acgagcttgt gctgttgtcg
     1561 gtgggcaatc agcaatggct aggcttttag gtgtatctcc tccaagcgta aatcaatgga
     1621 tcaaaggtgt acgtcagtta cctgctgaga gatgtcctgc gattgaacga gcaacaaaag
     1681 gtggtgttct gtgtgaagaa cttcgtcctg atgttgattg gacatactta cgacgctcgt
     1741 catgttattc gcagaatatg tcgatgaagc aaccaaatga cgaaaacgat catacccgaa
     1801 gcatcaagag gcaaatgatt catgaaaatc aaacatgagc acatccgcat ggcgatgaat
     1861 gcctgggcat accctgatgg tgagaaagtt cctgcagctg aaatagcccg gacttatttc
     1921 gaactgggga tgacgttccc tgaactgtac gacgacagcc atccggaagc cctggcccgt
     1981 aatacccaga aaattttccg ttggctggat aaagacaccc ctgatgctgt tgaaaaaatg
     2041 caggctctgt taccggcgat cgaaaaggcg atgccgcctt tgctggtggc ccgtatgcgc
     2101 agccacagtt ctgaatatta ccgtgagatc gtcgaacgga gggatcggct ggtgaaggat
     2161 gtcgatgatt ttgttgcgtc agcggttgtt ttgtatgacc agatgaatcg cggcggcccg
     2221 gcagggaatg ctgtggtgat gcactaaaag cacggtgttc gggggtttta tgagcagcaa
     2281 gcttcatggt cttgtctggg aagggtgcgc cttcaccggc atgatcttat ccagggtggc
     2341 ggttatggcc cgtcttgcag actacagcaa tgacgagggc gtgtcatggc ctgccattga
     2401 aactatccgg cgtcagatcg gtgcaagaag tgaatccaca gtgaaatcgg ctattgcaga
     2461 actggcgaaa gagggctggc tgacgaagga agagcgtaag gtcggtgggc gtaatgtaag
     2521 caatatctat cggcttaatg tggaaaaact cgaagcagct gcggcggcgg cgcgtgagtc
     2581 atataaaccg aaaagaaaaa ttagcccggc aaaaaatgac ccgttaacag ttgacccgtc
     2641 aaatattgac ccctcaacgg ttgacccgtc aaattttgat ggatcaactg ttgataaaaa
     2701 actgccgatt aggggggcga tgattgaccc cgatccgtca gtattaaaac ctgatccgtc
     2761 agataaaaga tcttcttgtc cggacgcttc gcaaccggac ccgcagacgg ctgaacagga
     2821 ttttttaacc cgacaccctg acgcggttgt gttcagtgcg aaaaaacgcc agtggggaag
     2881 tcaggaagat ttggtgtgcg cacagtggat ctggggacga atcgtgagtc tttacgagca
     2941 ggcggccagc gatgatggcg agatcactag accgaaagaa cccaactgga cagcatgggc
     3001 caatgacgtt cgcacaatgc ggatgctgga tggcagaact cacagacaaa tttgtgaaat
     3061 gtttgggcgt ctccagcggg attcgttctg ggtaaaaaac atcatgagtc cggcaaaact
     3121 ccgggaaaaa tgggatgaac tggttatccg cctggggcgt tcgcctgcgc agcgttgcgt
     3181 gaatcacatt tctgaaccgg acactgaaat accgccggga ttcagggggt gacgtgtcat
     3241 gaaaaacatt gcggcagttg gggttcttga acgtattcgc agacttgcac cacagggggc
     3301 ggttccaccg taccggacgg tggaggagtg gcgggaatgg caacttgctg aaggacgaaa
     3361 acgcagcgag gagattaacc gcctgaatca tcaggtgcgg gttgaaaaaa tcctgaaccg
     3421 tgcgggcatc cagccgcttc acaggaagtg ctcattcggg aactaccggg tgcagaacga
     3481 cggtcagcgc catgctctga gccaggcgaa atccattgcc gatgaattga tgaccggatg
     3541 tacaaacttc gtgttcagcg gtaaacctgg taccggtaaa aatcacctgg cagcagcgat
     3601 tggcaatcgg ctgatggcga aggggagaag cgtgattatc gtcaccgtgt ccgatgtcat
     3661 gagcgtgttg catgacggct acgacaacgg ccagtccggg gaaaaatttt tacaggagct
     3721 ttgtggagtt gaccttctgg tccttgatga aattggcatg cagcgggata cgcgcaacga
     3781 gcaggtcacg ctgaaccaga tagtcgaccg cagaacggct tcgatgcgta gtgtcggaat
     3841 gctgacgaac ctgaatcacg cagcgatgag cacactcctc ggagatcggg tgatggaccg
     3901 tatgaccatg aatggtggtc gttgggtgaa ttttaactgg gagagctggc ggtcaaacgt
     3961 tggacgtcag ggtatgtgag aatttttgac gaggtaaatt ttcgatggaa actgtattgc
     4021 atgcactgaa agcgatggga aaagccaatt ctgttgaact ggcggcgcgg cttgatatca
     4081 gccgtgaaga agttcttaac gaactgtggg aactcaaaaa aaatggcgtt gttgataaaa
     4141 cgggtcacac ctggtttctg gctgtcgaag gtgaagccgg ggtaaccgaa gggcaggcac
     4201 tacaacctga agcgccggat gtggtaaccg aagaggtcgc tccaaaagtt accgcagaca
     4261 tgatggttga gtttatcggt caggatggtg ctaaaacgtg tgaggaactg gcgggtaagt
     4321 tcggcgtcag tactcgcaag gttgcttcca cgctggcggt ggtaaccgca acggggcggc
     4381 tggcacgcgt taatcagaac ggtaaatttc gttactgcat gtcggggggt aatttaccag
     4441 cagatccgaa agccgcgccg gtaacgaaaa atgatggtaa ggcctttcct cagccagcag
     4501 gtgctgcgtt accagtccgg gaagccgcaa cacaggaaga aattaaaaca gaaactgtgg
     4561 cggacattgt gcagccgttg ctatcgttta ccgaaacgca agcagatgag ctgatttttc
     4621 cgtccctgcg cagggcaaac ctggcgctgc gcagggcgaa aagtgatgtt cagaagtggg
     4681 agcgagtctg cgccgcgctg cgggagctga acaagcaccg ggatattgtt cgacagatta
     4741 ctgattcttc ccgccgtgtt gtatcggaaa agtgatagcc ggaggcgctt atggcgaaac
     4801 cttttacaca cgaacagcgt gaagaactga aggcccgaat tatcgggctg gtacgcaaaa
     4861 atgaacgcat gacgatatca caactggaga gagcgacggg agcaggctgg cattcagtca
     4921 gacgttgcct tgtggatgta ctggcttgtg gcgatttata catgcccggt aaatacggtg
     4981 tttttacatc agaacaggtg tatcgcgtat ggcgtaaggc agcggagaaa gcaaccgacc
     5041 agacattgat tcgaaagtta ccagacggag aaatacgccg ctacgacaga caacagaaca
     5101 taatctgtgg cgagtgccgg aagagtgaag ttatgctgcg tgtactggcg ttctatcagg
     5161 gcaattttca ggaggcggta ctgtgagtga attagctatc aggcttcaat tgtcgctggc
     5221 attcgcatca aaggagaatg agatgatcac ttttacaaaa gagcagttaa tcagtcatgt
     5281 tagtgaaaat gtaaaggcga tgaaatttgc agtaaaacag acagtattca aaaattctct
     5341 cgaggcaatt gagttggatt tagcactggc ccttgttgct caggcttcgc tggaagcaga
     5401 gcccgtgctt tatatgaatc gatttaccgg aaagacattc tcactggaag agcaacccgg
     5461 tgctgataag gaaccggaaa tatacgtgcc gctatatgct gccccgccag acagcgccgc
     5521 catgcttcag gctggaaact ttcgggaaaa taagggttcg tcaaccaata attttcggga
     5581 aatctcggaa acgtcaacca actatccggt aactccggat ggttggataa gctgtagtga
     5641 gcgaatgccg gatgatggtc agcacgtaat tattttatgt gatggcgcat tcgttcttta
     5701 tgcgcaatat cgagacggtg agttttttga tgtagtccgt aatggtgatg aatttttcga
     5761 aacacagagc cgcaatgtaa ccgactggat gccgctacca gaaccgccgc aggaggtgcg
     5821 ccaatgatct ggcctgaagc ctttgcaatt acaggcgttg ctatagctat tgatttttta
     5881 gtatatgtta tttgtcggtg ggggtaaaaa cgttcgccgg gattcacacc aaaggaggga
     5941 atatgtcgga tgatatttca ctggcaatgg aaggtgctct ggctgttatt gctgttgtgg
     6001 gcgtttactg cctggttgtg tttttgatgg atcgactagg gaactgaatt cattacgata
     6061 tgggaattcc catatcgggt aaaaacggtt tgcggtaaag cgagagttaa gtagaattgc
     6121 tgcgggtgct tgaggctgtc tgcctcgggc atgccaccgt aaggcagaca gagaaaagcc
     6181 ccagttaaca ttacgcgtcc tgcaagacgc ctaacattaa tctgaggcca atttcatgct
     6241 agacatatgt aggttagcct cttacgcgcc gaaaggcaag gagaagcagg ctatgaagca
     6301 gcaaaaggcg atgttaatcg ccctgatcgt catctgtatc accgtcatag tgacgg
//
LOCUS       DAZFWP020000224         6128 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFWP020000224 DAZFWP020000000
VERSION     DAZFWP020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.226"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..842
                     /gene="exuR"
                     /locus_tag="ACHK0Z_RS23275"
                     /old_locus_tag="pgaptmp_004655"
     CDS             66..842
                     /gene="exuR"
                     /locus_tag="ACHK0Z_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU4504534.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1082..1165
                     /gene="yqiM"
                     /locus_tag="ACHK0Z_RS23280"
                     /old_locus_tag="pgaptmp_004656"
     CDS             1082..1165
                     /gene="yqiM"
                     /locus_tag="ACHK0Z_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU4504535.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1187..1849
                     /gene="yqjA"
                     /locus_tag="ACHK0Z_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             1187..1849
                     /gene="yqjA"
                     /locus_tag="ACHK0Z_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU4504536.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1853..2236
                     /gene="mzrA"
                     /locus_tag="ACHK0Z_RS23290"
                     /old_locus_tag="pgaptmp_004658"
     CDS             1853..2236
                     /gene="mzrA"
                     /locus_tag="ACHK0Z_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU4504537.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2383..2751
                     /gene="yqjC"
                     /locus_tag="ACHK0Z_RS23295"
                     /old_locus_tag="pgaptmp_004659"
     CDS             2383..2751
                     /gene="yqjC"
                     /locus_tag="ACHK0Z_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU4504538.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2789..3094
                     /gene="yqjD"
                     /locus_tag="ACHK0Z_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     CDS             2789..3094
                     /gene="yqjD"
                     /locus_tag="ACHK0Z_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU4504539.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3097..3501
                     /gene="yqjE"
                     /locus_tag="ACHK0Z_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     CDS             3097..3501
                     /gene="yqjE"
                     /locus_tag="ACHK0Z_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4504540.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3491..3790
                     /gene="yqjK"
                     /locus_tag="ACHK0Z_RS23310"
                     /old_locus_tag="pgaptmp_004662"
     CDS             3491..3790
                     /gene="yqjK"
                     /locus_tag="ACHK0Z_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU4504541.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3976..4368
                     /gene="yqjF"
                     /locus_tag="ACHK0Z_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             3976..4368
                     /gene="yqjF"
                     /locus_tag="ACHK0Z_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4504542.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4438..5424
                     /gene="yqjG"
                     /locus_tag="ACHK0Z_RS23320"
                     /old_locus_tag="pgaptmp_004664"
     CDS             4438..5424
                     /gene="yqjG"
                     /locus_tag="ACHK0Z_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU4504543.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5717..6082
                     /gene="yhaH"
                     /locus_tag="ACHK0Z_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             5717..6082
                     /gene="yhaH"
                     /locus_tag="ACHK0Z_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4504544.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1528 a         1516 c         1547 g         1537 t
ORIGIN      
        1 caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca ctccgggaga
       61 cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgccgct gacctgaaag
      121 agcgcatcga acagggcgtc tatctggtag gtgataaact gcctgcagaa cgctttattg
      181 ccgatgaaaa gaacgtcagc cgtacggtag ttcgtgaagc catcatcatg ctggaagttg
      241 aaggctatgt cgaagtgcgt aaaggttcag gtattcatgt ggtttccaac cagccgcgcc
      301 atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc
      361 aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac
      421 aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg
      481 attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc
      541 tggcggccat cgttgaaaaa atgtggaccc aacgtagcca taacccgtac tggaaaaaac
      601 tgcacgaaca cattgattcc cgtaccgtcg ataactggtg tgatgaccac gatcaaatcc
      661 tcaaggcgct gattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg cagcacctgg
      721 aaaacaccaa gatcatgtta ttcaacgaaa ccagcgacga cttcgagttc aatgccgacc
      781 gctatctgtt cgccgaaaac ccggtagtgc atctcgatac ggccaccagt ggcagtaaat
      841 gaaactcctg tccgacaggc gctgcgacgc gcctgtttgt ctcgtcggta agcgaaaagt
      901 ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcattcag caaaatcaga
      961 ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa
     1021 tttgaattta tgtttttgaa tgctttctta tctcacgatt taacagggaa tagttcaggc
     1081 tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg cggtagtgca
     1141 tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg aacttttgac
     1201 ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc catcgatgat
     1261 tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaacggct tgcttccagc
     1321 ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg cgaaaggcgc
     1381 gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg gctgctgggt
     1441 cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact ggctatctca
     1501 tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg gtttatcggc
     1561 gctgttaatt ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga ttgccgggtt
     1621 atcagggctg aataacgcgc gctttcagtt tttcaactgg atgagcggtc tgctgtgggt
     1681 attgatcctg acaactctgg gttacatgct cggcaaaacg ccggtatttt taaagtacga
     1741 ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt ttggcctggc
     1801 aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat
     1861 acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc tggtcggcac
     1921 gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga ttcgtgccgt
     1981 tcatcaaggc acaacgatgc cagacggttt ttcgatctgg catcaccttg acgctcatgg
     2041 cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat ttgactccag
     2101 cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg gctacatcat
     2161 tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac gggataactc
     2221 tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg tgatttcacc
     2281 gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt ctggacaatc
     2341 gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata ccgcatcgct
     2401 ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa
     2461 aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt
     2521 attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga tagccagctg
     2581 cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga acgccagcaa
     2641 gatttagccg aggcgaagca aaaaggcgat gccgataaga ttgccaaacg cgaacggaaa
     2701 ctggcagaag cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta actcacaata
     2761 gtcactactt actcacctgg agaaaactat gtcgaaagaa cacactacgg aacatctgcg
     2821 tgctgagttg aaatcccttt ccgatacgct ggaagaggtg cttagctcat ctggcgagaa
     2881 gtcgaaagaa gagttgagta agattcgtag caaagcggag caggcactga aacagagccg
     2941 ttatcgcctg ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg cggcggcgcg
     3001 tgctgatgag tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg ctgcaatcgg
     3061 tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca tcacgcgcaa
     3121 gggcccggta aaagcgttct gggcatcggg cagcgaattg tttccatcat ggttgaaatg
     3181 gtggaaacac gtctgcggct ggcggtggtg gagctggaag aggaaaaagc taatctcttt
     3241 caacttttac tgatgctggg cctgacgatg cttttcgctg catttggtct tatgagcctg
     3301 atggtgctaa ttatttgggc ggttgatccg caatatcgcc tgaatgcgat gattgccacc
     3361 accgtggtgt tgctgctact ggcactgatt ggcggtatct ggacgttacg taaatcgcgt
     3421 aaatccacct tgctgcgcca tacacgccat gagctggcaa acgatcggca actgctcgag
     3481 gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac tgcttagcca
     3541 gatccagcaa caacggctgg atctttccgc cagtcgtcgt gaatggctgg aggcaacagg
     3601 cgcttacgat cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc tggttggcag
     3661 tagcgtgatg gcgatctgga cgattcgcca tcctaatatg ctggtccgct gggccagacg
     3721 cggttttggc gtatggagcg cctggcgtct ggtaaaaacg accctcaagc agcaacagct
     3781 tcgcggttaa cttccctctg gccggagcaa ttccggcctt atccctcaaa ttttttgaag
     3841 atttttgaca gttttccttg ctaacaatca tcattcacca cgtttatgat tctctccatc
     3901 gacagcaacg acgctaatac cgcgcccttg cacaaaaaaa caatcagcag cctgagtggc
     3961 gcagtggaga gtatgatgaa aaaattagaa gatgttggtg tactggtagc gcgcatttta
     4021 atgccgattc tgtttattac cgcaggctgg ggaaaaatca ccgcttactc tggtacccaa
     4081 caatatatgg aagcaatggg tgtcccgggt tttatgctgc cgctggtgat tctgcttgag
     4141 ttcggtggtg gtctggcaat cctgttcggt ttcctgactc gcaccacagc cctgtttact
     4201 gcgggcttta cgctgctgac ggcattttta tttcacagca actttgctga aggcgtcaac
     4261 tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat tcctgctgct ggcaattacc
     4321 ggtccgggcg cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc acgcgctata
     4381 cttaacgata aaaaacgagg aggaagctcc tcgtttttgc tattggagga gagaaaaatg
     4441 ggtcaactga ttgacggcgt ctggcatgac acctggtacg ataccaaatc taccggcggt
     4501 aaatttcaac gttcagcttc cgcatttcgt aactggctca ctgccgatgg cgcacctggc
     4561 cccactggca caggcggttt tatcgcagag aaagatcgtt atcatctcta tgtttcactc
     4621 gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac tcaaaggact ggaaccgttt
     4681 atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt tgatgacagt
     4741 tttccgggag caaccggaga tacgctctat caacatgaat ttctgtatca gctttatctc
     4801 cacgccgatc cacactacag cggacgagtt actgttcccg tgctgtggga caaaaagaac
     4861 cacaccatcg tcagcaacga atcagcagaa atcatccgca tgtttaatac cgcgtttgat
     4921 gcgctgggcg cgaaagcggg tgattactac ccaccagccc tgcaaacgaa aattgacgaa
     4981 cttaacggct ggatttatga caccgttaac aacggcgtgt ataaagctgg ttttgccacc
     5041 agccagcaag cctacgacga ggcggtggcg aaagtgtttg aatcgctggc gcgactggag
     5101 cagattttag gccagcatcg ttacctgacc ggcaaccagc taaccgaagc cgatattcgc
     5161 ctgtggacca cgctggtccg ttttgatcca gtgtatgtga cccacttcaa gtgtgataag
     5221 caccgcatca gcgattacct taatctgtat ggcttcctgc gtgatatcta ccagatgccg
     5281 ggaattgccg aaacagtcaa tttcgatcac atccgtaatc attacttccg cagccataag
     5341 accatcaacc ctacggggat tatttcaatt ggtccatggc aggatctcga tgaaccgcat
     5401 ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg aggtgcctct gataattagc
     5461 taagtgcatg ttgttcatgt cggatgcggc gtgaacgcct tatcaaacct acaaaacatt
     5521 tatattcaat atgttgcaat gaccgcgcag gccagataag cgtagcgcat ctggcgattt
     5581 tgcgcttgtc atcagtctta gcgcgccttc acagcacttt attaattctt tgaacgaata
     5641 tttactgcca tttcattcac gtctattctt aatttgctgc tttaaaaaca agtgattgag
     5701 caaaattgag gcaaaaatgg actggtatct gaaagtacta aaaaattatg tcggtttccg
     5761 gggtcgtgcg cggcgcaaag agtactggat gtttattctg gtcaacatca tctttacgtt
     5821 cgtgctgggg ctgctggata aaatgttagg ctggcaacgc gctggtggcg aaggcatcct
     5881 gacgacaatc tacggtattc tggtgttttt accgtggtgg gcagttcagt tccgccgcct
     5941 gcacgacacc gaccgctcgg cgtggtgggc actgttgttc ttaatcccgt ttatcggctg
     6001 gcttattatc atcgtcttta actgccaggc gggtacgcca ggcgaaaacc gctttggacc
     6061 agatccgaaa ctggaacctt aaaacaaatc tgctcgccgg atgtctgact catccggctt
     6121 ttatcatc
//
LOCUS       DAZFWP020000225         6068 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFWP020000225 DAZFWP020000000
VERSION     DAZFWP020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.227"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            170..898
                     /gene="gadW"
                     /locus_tag="ACHK0Z_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             170..898
                     /gene="gadW"
                     /locus_tag="ACHK0Z_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU4504545.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHK0Z_RS23335"
                     /old_locus_tag="pgaptmp_004667"
     CDS             complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHK0Z_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU4504546.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLCTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHK0Z_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHK0Z_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU4504547.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5616..5894
                     /locus_tag="ACHK0Z_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             5616..5894
                     /locus_tag="ACHK0Z_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504548.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5895..>6068)
                     /gene="gadE"
                     /locus_tag="ACHK0Z_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             complement(5895..>6068)
                     /gene="gadE"
                     /locus_tag="ACHK0Z_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU4504549.1"
                     /translation="NQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRR
                     MSDIVTLGITSYF"
BASE COUNT         1492 a         1508 c         1522 g         1546 t
ORIGIN      
        1 tattcgtgct gcatttactt attatcaatt aactgttatg caaaactact ttgtggataa
       61 attttggtcc taccaaatct ggcagttttt tcgctaagaa acagtctggc aacatttcat
      121 tagtatactg aaattgaaat aatcgcagta tgaaatataa gggataatca tgactcatgt
      181 ctgctcggtg atcctcattc gtcgttcatt cgatatttat catgaacagc ataaaatatc
      241 gctgcataac gagagtatcg tgctgctgga gaaaaatttg gcagacgatt ttgcgttttg
      301 ttcaccggat acgcgacgac tggatatcga tgagctgaca gtttgccatt acttacaaaa
      361 tattcgtcag ctaccacgca atttagggtt acatagcaaa gaccgtctat taattaacca
      421 gtcacccccc atgccgctgg tgacggcgat ttttgatagc ttcaatgaat ccggggtaaa
      481 ttcaccgata ctgagcaata tgctctacct ttcctgttta tcgatgtttt ctcataagaa
      541 agaactgatc cccttacttt tcaatagcat cagcactgtt tcaggaaaag ttgaacgcct
      601 tattagcttt gatatcgcca aacgttggta tctgcgcgat atcgcggaaa gaatgtatac
      661 cagcgagagt ctcatcaaaa aaaagttgca ggatgaaaat acctgtttca gtaaaatatt
      721 actcgcttcc aggatgtcga tggccagacg attactcgag ttacgtcaaa ttcctctgca
      781 tactattgca gaaaaatgtg gctatagcag tacgtcgtac tttataaaca catttcgaca
      841 atattatggt gtaacgccac atcagtttgc gcaacattcg ccaggtacct tttcctgaca
      901 tattttgcat ttgaatattg gtcaggatct cacacctgct tcatgtgaaa ctcttccctg
      961 atgatttctg ccgggctacc ggctagttct ctttcgcagt ttgccaggcg gtataggtat
     1021 tttcaggagg aaaagactcg gcatgtttgg gattattaag ctgacaattc atacgattac
     1081 gggaatgtgg gtcagtattg tgctgttcaa attgatgacg aatggctgga gtggtttcta
     1141 ctttcagtgc tgtgtactaa gcctggtatt cctgacggtt agctggttgt tgagtggtga
     1201 atggttagca ggaaagagta aggctgaacc ttcacgttca accttactct catttacacg
     1261 ttacgctttt ttaaagcggg caaagagatg ttccactaca acgaaaaaga ccggaacgaa
     1321 gtaaattgcc agcactgttg cggcaaacat cccgcccatc acgccggtac ctaccgcgtt
     1381 ttgcgcgcca gaaccggcac catgactgat aaccagcggc agcacgccga gaataaaggc
     1441 cagagaggtc atcaggattg ggcgtaaacg catccgcgcc gcttcgatga ttgcctctat
     1501 cggcgttttc ccttctttct gcatcatctc aacggcaaat tcgacaatca ggatggcgtt
     1561 cttggcggaa agcccgatgg tggtcagcaa accaacctgg aagtagacgt cattacttaa
     1621 gccgcgcaga tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac
     1681 cgagaacgga attgaccagc tctcatagag tgcggcgagg gcgaggaaca ccacgaccag
     1741 tgaaatggca tacagtgcag gagcctggtt tgaggataac gcttcctgat acgacagtcc
     1801 ggtccatgag tagccgacgc ctgccggaag tttagcgacc aggtctgcca taaatttcat
     1861 agcgtcaccg gtacttttcc cggccgccgc ttcacctaaa atctccattg acgggatgcc
     1921 gttgtagcgt tccaatcgcg gtgaaccata ggtccattcg gtagacgagt aggcagaaag
     1981 cggtgccatc gtgccagagg cgttgcgtac ataccattgg ttgatgttat ccggcaacat
     2041 acggaacggc gtgcctgcct ggacatacac ttttttcacc cgcccctggt tgaggaagtc
     2101 gttcacgtag ctgctgccga aggcggtgga aattgtctgg ttgatatcag acagcgccac
     2161 acccattgcc tcagctttcg cggcgttgac gttcactttg aacatcggcg tatcttccag
     2221 gccgttcggg cgtaccccgg tgacctggtc aggcgattgc gctgccagtg ataacagctc
     2281 gtttcgtgcc tgggttagtt tttcgtgccc caggttaccg ttgtccagca gttccatatc
     2341 aaaacctgac gcggtaccca gttcagccac cgcgggtaag ttgaacggga agacgacggc
     2401 tttattgata ctgcttaacg caatcattgc ccgctgaatg atcgcggtaa ccgagttttc
     2461 ctcaccgaca cgttcagacc acggcttgag actgataaac gccaggccgt tgttttgccc
     2521 ctggccgctg aagccaaagc cgccaacggt aaacaccgac tgaacattat ctttctcttt
     2581 tgtaagataa taatccgtca cctgttgcag cactttcgtg gtgttaacca tggtggcacc
     2641 ggaaggtaac tgcgcggtgg tcataaatac cccctgatcc tcttctggta agaaagaggt
     2701 cggtgtacac aggaacagca ccgccatccc ggcgcaaatc agcaggtaga ccaccatgta
     2761 gcgaccggta caacgcaaca gcgagcgggt gctatcggta tagtgctgag ttgatttttc
     2821 aaacagcgtg ttgaagcgtg cgaacagggc gttaggtttg tgaccgcctt ccggcgcggc
     2881 tttcagaatg gtggcgcaca gggcaggggt caggctcatt gccacaaata ctgaaagcag
     2941 catggaggag atcagcgtga tggagaactg gcggtagatc tccccggttg cgccgctcat
     3001 aaaggccatc ggcataaaca ctgcggaaag aacaacagca ataccgacca gcgcacgttg
     3061 gatctgcccc atcgatttat gcgtcgcttc cttcggtggt agcttatctt ccgcaatgac
     3121 acgctcgacg ttctccacca ccacgatggc gtcatccacc agtagcccta tcgccagcac
     3181 catcccgaac atagttaacg tgttgatggt gaaaccgacc gccgacaaga tcgcaaacgt
     3241 cccgagaata accaccggta cggcaatcgt cgggatgatt gtggcacgga aattctgcaa
     3301 aaacagatac atgaccagga agacgaggat gatagcctca accagtgttt taaaaacttc
     3361 ctgaatagaa atttcgataa acggcgtggt gtcgtaagga taaaccgtct tcagacttgc
     3421 ggggaaatag gctgataagc ggttcagctc ctctttgact gcccgcgagg tatccagggc
     3481 gtttgctccg gtagccagtt tgatggcgat cccggcagca ggtttgccgt tatagcgcgc
     3541 cacggtggaa taatcttccg ctccaagttc gacgcgggcg acatcacgca gcagcacttg
     3601 cgaaccatct tgctgaactt tcaacaggat tttgccaaat tcttccggcg tttgcagacg
     3661 cgtctgcaca atgatcgagg cgtttagttg ctggtctgcc gcctgtggca tgccacccag
     3721 ttgaccaccg gaaatctggt tgttttgcac cttaatctgg gaaataacat cggaaggtac
     3781 cagattgtat ttattgagtt tttgcgggtc cagccagata cgcatggcgt actcggaacc
     3841 aaagagctgt acgctaccaa cgcccgcggt acggcttagc gggtctttga tattagacgc
     3901 tacatagtcc gcgatatcgt actggttgag gctgccgtta tcagaaataa acgccgctac
     3961 catcagaata ttactgctcg acttatcaac gctaatcccc tgctgctgca ccgcttcagg
     4021 taatgaaggc atagcgagtt gcagtttatt ttgcacctga acctgtgcga tatcaggaga
     4081 tgtcccagtc tcgaaggtca gagtgataga ggcattaccc gccgcatcac tggttgaaga
     4141 catgtacatc aggccatcaa gcccattcat attttgctca atcacctgag tgaccgagtc
     4201 ttctaccgtt tgcgcatcgg cacctggata ggtagcgctg acggtaatgg tcggtggcgc
     4261 aatctgcgga tactgcgcaa ccggtaagtt catgatcgcc agaccacctg caagcatcat
     4321 aataatggca agtacccagg caaaaaccgg gcgatcaata aaatagttag ccatgaaagt
     4381 ccccttaagc ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct ggctggagga
     4441 aattgctcgt gctttgatac ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc
     4501 gccagcctgc aagccagagg tgacaaccca ctggtcgccg atggctttgc tggcttcaat
     4561 ttcgcgtagc tgcacaacat cgtctttatc cagaatgagc gccgttgctt taccctgggc
     4621 gttgtgggtg acgccttcct gcggcaccag taatacattc tggcggctac cttcatccac
     4681 taatgccgtg acgtacatgc caggcagcaa gtcaccattt gggttgggga aaatcgcccg
     4741 taacgtcacg gagcccgtgg tttcatccac cgtcgggtcg gagaatttca gcgtgccggt
     4801 ctggctgtag cgtttaccat tttccagatt gagctgtact ggcgtactgc cctgaacctg
     4861 tttgatttgc ccactggcga cctcttcttt catgcgtaag aaatcctgca ccgactgcgt
     4921 gagatcgaca taaatcgggt ccagacgttg tacggtaacc agcgaatctg cctgattagc
     4981 ggtaacgagt gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt
     5041 gacattcgcg tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac
     5101 attggcttct gcttcattca actgggtgcg cgcggtgtcg taatcctgac ggctaacgta
     5161 gttggtcttc agcaacgatg cctggcggtt aaaggtgatg cgggcattgc tggcggtaga
     5221 gagcgctttc gccagcgagc ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc
     5281 aatctgatac agcgaatcgc cctggttcac tttatcgcct tcgataaagt tgcgtttaat
     5341 gataataccg cccacctggg gacgtatctc ggcaacttca taaggaacgg ttctaccggg
     5401 caattcgctc aacacattga ccgaaccggg ggagagtgtg acgacaccga cctcaggcgt
     5461 catggcggcg gcgttttccg ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa
     5521 aaacaacggt attaacagct ttcttcttct gttcatttta gtccctgaaa attcttgaga
     5581 aataacggtt ctttaaattg cgcgcgagga gagatatgag ggatttacag accagcggca
     5641 tagtggggtt gtcagccagt aaggtgggat acaggcacag tgatcgacat ggtgaggtca
     5701 acgacaaaaa aaatgttgcc gttctgccaa cagttcctgc cagcattcgg gcaacgaagg
     5761 gttctactgg tggatacaca taccagggga ataaagtcgt tttagcctct cctttagtaa
     5821 tcaccggggg taatgaaatc agcatctgca tccctcgtca tgccagccat caatttcagt
     5881 tgcttatgtc ctgactaaaa ataagatgtg atacccaggg tgacgatgtc gctcatacgt
     5941 cttagtttca gcttgtacat aatgttgaat ttctgaatct ttaatgtttg ttgcgaaata
     6001 ttgagcgtcg acgtgatatt gctttcattt ttatttttca gaatcagttg cataattttg
     6061 aactgatt
//
LOCUS       DAZFWP020000226         6051 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFWP020000226 DAZFWP020000000
VERSION     DAZFWP020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.228"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..541
                     /locus_tag="ACHK0Z_RS23355"
                     /old_locus_tag="pgaptmp_004671"
     CDS             <1..541
                     /locus_tag="ACHK0Z_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4504550.1"
                     /translation="KKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDE
                     DLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQT
                     DLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPS
                     KDATPGNVYAQADYKIAYE"
     gene            601..3105
                     /locus_tag="ACHK0Z_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     CDS             601..3105
                     /locus_tag="ACHK0Z_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4504551.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3121..3870
                     /locus_tag="ACHK0Z_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             3121..3870
                     /locus_tag="ACHK0Z_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4504552.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCQTVNNSCNVSVAKRDK"
     gene            3872..4936
                     /locus_tag="ACHK0Z_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             3872..4936
                     /locus_tag="ACHK0Z_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4504553.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4979..5179)
                     /gene="glgS"
                     /locus_tag="ACHK0Z_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             complement(4979..5179)
                     /gene="glgS"
                     /locus_tag="ACHK0Z_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU4504554.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5448..>6051
                     /locus_tag="ACHK0Z_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             5448..>6051
                     /locus_tag="ACHK0Z_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4504555.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAE"
BASE COUNT         1848 a         1267 c         1216 g         1720 t
ORIGIN      
        1 taaaaagtca cttcttgttg ctggcgtagc aatgattctt tccaataacg tatttgctga
       61 cgaaggtcac ggcattgtta aatttaaagg tgaagttatc tctgctccat gttcaattaa
      121 accaggtgac gaagacttaa ctgtaaatct gggtgaagta gcagataccg ttctgaaaag
      181 cgaccagaaa tctttggctg agcctttcac tattcacctg caagactgca tgttgagcca
      241 gggtggaacc acttattcaa aagcgaaggt tactttcacc acagcgaata ctatgacggg
      301 tcagactgac ctgctgaaaa acaccaaaga aactgaaatc ggtggtgcca caggcgttgg
      361 cgtacgtatt ctggatagcc agagcggcga agtgacttta ggtactccgg tagtaattac
      421 attcaacaat actaacagct atcaggaact gaacttcaaa gctcgtatgg aatccccaag
      481 caaagatgct accccgggca acgtttacgc tcaggctgac tataagattg cttacgagta
      541 atcacattag gccgggttcg cccggccaca tcattaacgg attaatgata agtggaacag
      601 atgtataaaa aattaaaatt aaccacaata agcgaattga ttaaaaatat ttattgttca
      661 ttatccgttc tcatcattgg ttgtgcgtca gcttatgccg ttgaattcaa caaagattta
      721 atcgaagccg aagatcgtga aaacgttaac ctttcccaat ttgaaactga tggccaatta
      781 cccgtaggta aatactcatt aaacgctctt attaataata aaaggacgcc aatccatctg
      841 gacctgcaat gggtattaat tgataaccaa actgcagttt gtctgactcc agagcaatta
      901 acattattag gctttactga tgagattatt gaagtggctc agcaaaacct gatcgatggt
      961 tgttacccta tcgaaaaaga aaaacaaatt acaacttatc tcgataaagg gaaaatgcaa
     1021 ttatccatat ctgcacctca ggcatggtta aaatacaaag atgcaaactg gacgcctcct
     1081 gaactttggg atcatggtat tgctggggca tttcttgact acaatttata tgcctctcat
     1141 tatgcaccac atcagggcga taattcacaa aatataagtt cctatgggca ggctggggtt
     1201 aatcttgggg cctggcgcct gcgtactgat taccagtacg atcagtcatt taacaatggc
     1261 aaaagccagg cgaacaacct ggattttccg cgtatttatt tgtttcgccc aatcccagca
     1321 attaatgcaa aactaactat aggtcaatac gatactgaat cctctatttt cgactctttc
     1381 catttttctg gcgtttcgtt gaaaagcgat gagaatatgt tgccgcctga tctacgtggt
     1441 tacgcaccgc aaatcacggg tgttgcacaa acgaatgcaa aggtcactgt ctcacagaac
     1501 aaccgtatta tttatcaaga aaatgttcct cctggcccat ttgctattac taatttattc
     1561 aatacattac aggggcaact ggacgtcaag gttgaagaag aggacgggca agtcacgcaa
     1621 tggcaagttg catctaatag tattccttat ctgacgcgta aagggcagat tcgctacacc
     1681 actgctatgg gtaaaccgac cagcgttggt ggtgattcct tacaacaacc cttcttctgg
     1741 actggtgaat tctcatgggg ttggctgaac aatgtatccc tgtatggtgg ttcagtttta
     1801 acaaaccgtg attatcaatc tctggctgcc ggcgttggct ttaaccttaa ctcattgggt
     1861 tcattatctt ttgatgtcac acgatctgat gctcagttgc ataatcagga taaagaaacg
     1921 ggttatagct accgcgctaa ctattcaaaa cgttttgaat ctaccggtag ccagctcact
     1981 ttcgctggtt accgtttctc tgataaaaac tttgtgacaa tgaatgaata tatcaatgac
     2041 actaaccatt acacgaatta tcagaatgaa aaagagagtt atattgtcac gtttaaccag
     2101 tatcttgaat cattaaggtt aaatacatac gtaagtttgg ctcgtaatac ttactgggac
     2161 gccagcagta atgtgaatta ttcattatca cttagccgcg attttgatat cgggccatta
     2221 aaaaacgtct ccacttctct aacatttagc cgaataaact gggaagaaga caaccaggat
     2281 caactgtacc taaatatttc gattccctgg ggaaccagta gaacattgag ctatggtatg
     2341 caacgaaatc aggataacaa gatttcgcat actgcttcgt ggtatgactc ttccgatcga
     2401 aataattcct ggagcgtttc tgcttcaggc gacaatgatg aattcaaaga tatgaaagcg
     2461 tcactacgcg ccagttatca gcataatacc gagaacggtc gtctctatct ctccgggaca
     2521 tcacagcgag acagttatta ttctctgaat gccagttgga atggttcatt cacggcgact
     2581 cgtcacggag ccgctttcca tgactatagt ggtagtgctg actcgcgttt tatgatcgac
     2641 gcagatggtg cagaagatat tccgttgaac aataaacgcg cggtaactaa tcggtatggc
     2701 atcggagtta ttccatcagt cagcagttac ataacaacat cattaagcgt tgatacccga
     2761 aatcttccag aaaatgtgga tatcgaaaac tcggttatca ccaccacctt aaccgaaggt
     2821 gctattggct acgccaaact tgatacccgc aagggctatc aaatcatggg ggttattcgc
     2881 ctggcagatg gtagtcaccc accactgggg attagcgtaa aagataaaac cagccataaa
     2941 gaattaggac tggttgctga tggcggcttt gtatacctca acggcattca ggatgacagc
     3001 aaacttactt tacgctgggg tgataaatcc tgttttattc aaccacccaa tagcagcaac
     3061 ttaaccaccg gaacggttat tttaccgtgt attagccaaa attaattcag gatgaaacta
     3121 atgcgctact taaacacaaa gaatctcatt gcagcaggag tattactcgc ctgcatgagc
     3181 agcattgcct ggggtgccat catcccggac cgtacccgta tcataatgaa tgagtcagat
     3241 aaaggggaag cattaaaact tactaatcaa agcaaaaatc taccctatct ggcgcaaaca
     3301 tggatagaag atacgaaagg caataaatca cgtgacttta ttgtcacggt tccccctatg
     3361 gtacgtttaa atcccagtga gcaaattcaa ataagaatga taagtcagga aaaaattgct
     3421 caactgccta atgacagaga aacgctcttc tattttaacg tgcgagaaat accgccaaaa
     3481 acagataaaa aaaatgtgat gcaagtcact atgcagcacg cattgaaact attctggcga
     3541 ccaaaagcta ttgaattaga agatgatggg gtaatgtctt acgaaaaagt tgaaatcatc
     3601 cgtagaaatg atggttcgat ccgctttaac aataagatgc cttatcacgt tacgctgggt
     3661 tatatcggaa caaacggaat cacaatgttg ccacaaacgc aaagtttaat ggtaacccct
     3721 tatagccatg cagatacgca gtttaaaaat gttccgtcag cttttcaggt tggttatatc
     3781 aatgattttg gtggattaag tttttatgaa ataaactgcc agacggttaa taactcttgt
     3841 aatgtttccg tagccaaacg agataaataa tatgcgctac ttattaattg taattatatt
     3901 tattataggt tttagtgcat tacctgtatg ggcaatggac tgctatgctg aacatgaagg
     3961 gggaaatacc gtagtcattg gttacgtacc aagaatttct atccccagta atggtaaaaa
     4021 aggtgataag atctggcaaa gcagtgagta ttttatgaat gtcttctgta ataatgcatt
     4081 acctgcccca tcacctggag aagaataccc atcagcctgg actaatataa tgatgttttt
     4141 accaggcggt caggactttt ataatcagaa ctcctatatt tttggcgtaa catacaatgg
     4201 agttgattat gattcgactg cccctaatgc tatcgcagca ccagaatgca ttgatataaa
     4261 aggagcgggt acctttaata atcactataa aaaccccgct gtctgtagtg gaggtcctga
     4321 gcctcaatta tcagtaacct ttccagctcg cgtacaactc tatattaaat tagcaaaaaa
     4381 tgccaataga gttaataaaa acctggtgtt acctgatgaa tatatcgctc tagagtttaa
     4441 aggtatgagt ggcgcaggag ctatagaagt agacaaaaat ttgaccttca ggattcgtgg
     4501 attaaataat attcatgtcc ttgactgctt tgttaatgtc gcactggaac cagctgatgg
     4561 cgttgttgac tttggtaaaa taaattcccg aacaattaaa aataccagcg ttagtgagac
     4621 gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat tcaatatttt
     4681 agggagcttt ttcactacgg atattttgag tgattatagc catctggata tgggtaatgg
     4741 actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc gcttctcaca
     4801 atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca gggcagaact
     4861 tagtgcgaat ccggcagaga ctgttgttga gggaccgttt agtaaagacg tcatcctcaa
     4921 aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc ccgttatatc
     4981 agtgctctaa ctccagctct cttgcctgca tcatctgttg acaataccag gcataacgtg
     5041 cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggcaaga atatcgaccg
     5101 ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa ttatttaaag
     5161 aattaagact atgatccata agcacatcct ccggttatcg catacctttt aaggccatat
     5221 gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata acgtgcgtaa
     5281 atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta aaaaactttc
     5341 tcttccagaa cttctctaac atgtgttatt attaacaaag ataaatgtaa gcaaggaaac
     5401 tatcccaaag taaatattgc gtcactaaat ggacattgga gtgacatatg attttattcg
     5461 ccgactataa taccccttat cttttcgcta tttcctttgt actgttgatc ggccttttag
     5521 aaattttagc gcttatctgc ggtcacatgc tctccggtgc tcttgatgca catcttgacc
     5581 attatgattc cataaccaca ggtcatatta gccaggcact tcattatctc aatatcggaa
     5641 gattgccagc tctcgtcgtt ctctgcttgc tggctggttt ttttggtctt attggcatcc
     5701 tgttgcagca tgcctgcatc atggtctggc aatcaccact gtcgaacctg ttcgtggttc
     5761 ctgtcagttt actgtttacg attattgcgg tgcattatac cggtaaaata gttgcgccct
     5821 ggattccacg cgaccatagt tcagctatta cagaagaaga atatattggt agcatggcat
     5881 taattaccgg tcatcaagcc acttcaggta atccctgcga aggaaaactc accgatcaat
     5941 ttggccaaat acattattta ctactggagc ctgaagaagg aaaatttttc actaaaggag
     6001 acaaagtgtt aattatttgc cgactttctg caacgcgata tttggcggaa a
//
LOCUS       DAZFWP020000227         5957 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFWP020000227 DAZFWP020000000
VERSION     DAZFWP020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.229"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..670
                     /locus_tag="ACHK0Z_RS23385"
                     /old_locus_tag="pgaptmp_004677"
     CDS             <1..670
                     /locus_tag="ACHK0Z_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4504556.1"
                     /translation="GTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVI
                     LLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSG
                     IDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAVVKNTAGAG
                     DAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQT
                     IFTH"
     gene            684..1442
                     /locus_tag="ACHK0Z_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             684..1442
                     /locus_tag="ACHK0Z_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HGU4504557.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPDHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            1499..2464
                     /locus_tag="ACHK0Z_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             1499..2464
                     /locus_tag="ACHK0Z_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4504558.1"
                     /translation="MKKNKYSTPLWVLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKEIRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            2467..3501
                     /locus_tag="ACHK0Z_RS23400"
                     /old_locus_tag="pgaptmp_004680"
     CDS             2467..3501
                     /locus_tag="ACHK0Z_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HGU4504559.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQ
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(3541..3833)
                     /locus_tag="ACHK0Z_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /pseudo
     CDS             complement(3541..3833)
                     /locus_tag="ACHK0Z_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(3913..5532)
                     /locus_tag="ACHK0Z_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /pseudo
     CDS             complement(3913..5532)
                     /locus_tag="ACHK0Z_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5816..>5957)
                     /locus_tag="ACHK0Z_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     CDS             complement(5816..>5957)
                     /locus_tag="ACHK0Z_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HGU4504560.1"
                     /translation="DYTVKVRKWFCVMCDENYEGEKCCPSCGTGVYSREGGQEGNWKD
                     RN"
BASE COUNT         1547 a         1457 c         1365 g         1588 t
ORIGIN      
        1 cggaactgcc acgatccttg ttaatgaagg tactggcgat aatgttattg cgatctatcc
       61 cggtgccaac atgacaatgt cgtccgatga aataactatt cagaaagaag cgattattaa
      121 ttctgatgtc atacttctgc agctcgaaac aaattatacc gctctgcaac aagcgatcac
      181 attggcgcaa aaaaacagta tcccggtcat tattaacccg gcaccctata atgatatcgt
      241 caacgaactt attcaggatg ttgattatat cacccccaat gaaacagaag ctggtttact
      301 gtccggcata gacgtacacg atcttgaatc agctaaacga gctgcagaag ctatccacaa
      361 taaaggggta aaaaacaccg ttatcacgct cggtagcaag ggctcgctcg catttgatgg
      421 caaaaaattc attcattcgc cagcgtttcc agcggtcgtt aaaaatacgg caggagccgg
      481 ggatgctttt aacggcgctc tggcatcagg acttgcgaaa ggaaaatcac tggaatcagc
      541 actgtgttat gccagcgcct ttgcatccct agcggtggag acaagcaatg cttccgacat
      601 gcctgaacat gaatctgtga tacatcgaat tcagagtata cattatcagc aaactatttt
      661 tacgcattaa gaagggacag agaatgaata acaccaaaaa atcactaaag gtgctcttta
      721 ttggagaatc ctggcatatt catatgattc attcaaaagg atatgacagt tttacctcaa
      781 gtaaatatga agaaggtgcg acctggttac tccaatgtct gaaaaatagt caggtagatg
      841 tcacctatat gccggcacat acggtacaaa ttgccttccc tgaagatgtg gcacagttag
      901 aacaatatga tgccatcgtt attagtgata ttggtagtaa tactttcctg ctacaaaatg
      961 atacttttta tcagcttcgc ataaaaccaa acgctctcga actgattaaa gaatatgtga
     1021 ataacggtgg tgggttattg atgattggcg gctatctctc attcatggga attgaagcaa
     1081 aagccaatta taaaaataca gttctggctg atgtgctccc ggttacgatg ttagatggtg
     1141 acgatcgtgt cgaaaaacca gagggtgtta ttgcacagcc atcacaacct gaccatccgg
     1201 ttattaaagg gttctctgaa tatccttttt tcctaggtta taaccgggcg atcgctaaag
     1261 aaaatgctga ggtggtatta acaataaaca acgatccatt actggtcttt ggtaactatc
     1321 acaacgggaa aatagcctgc tttatgagtg actgttcacc gcactggggt acacagcagt
     1381 ttatgtcctg gccgttctat accgcgttgt gggtaaatat actgactcat attgcaagat
     1441 aataataata atggggtaac accagttact ccatttacta taatagaagg attgatgtat
     1501 gaaaaagaat aagtactcaa ctcccttatg ggtgcttgcc acgattcttg cgggtatgct
     1561 ctcccctatg caatcagcgg tgaatggtca attaggtcac tggttacagg acggtaatgc
     1621 atgcgcagtt atctcctttg ccagtggttt agtcgtgatg ttttttatta ttatggcgcg
     1681 aaaagaaatc cggcaacaat ttgcggcaat tccttctctg attaaaaacc gaaaagttcc
     1741 tctctggaac tggtttgccg gtctctgtgg agcgatggtg gtattttctg aaggcgcatc
     1801 agccagcgca ctcggggtcg caacgtttca gaccgcactg atctccgcgc tattgctgtc
     1861 tggtttactt tgcgatcgct ttggtatcgg tgtcgacgag aaaaaatact ttacccctta
     1921 tcgcatcatc ggtgcattat ttgcggttat tgctacgatt tttgttgtgt caccgcagtg
     1981 gcattctacc tctttcattt tacttgccat tcttccgttc ttagctggtt tgcttgcagg
     2041 atggcagccc gctggaaatg caaaagtagc tgaagctacg ggatccatgc tggtttcaat
     2101 cacctggaat ttcatcgttg gattttgtgt tttaggcaca gcgctagcta ttcgtgtcgc
     2161 attagggcac gtcactgttc aattaccaga tgtctggtgg atgtatcttg gcggcccgct
     2221 tggactaatg tctatcggtt taatggccat tttggtcagg ggtctggggt tactgatgct
     2281 gggggttgct tcaacggcgg gccagttact gggatcagta ttgattgacg tattaattcc
     2341 atcgctgggc aataccgttt atctggtcac gatcatcgga acactgtttg cactggtcgg
     2401 tgcaattgtt accactattc cggaatacag agcctcgaaa acaatgaaga aaatggaggt
     2461 ataatcatga aagattatct acaaactgtg acgggccccg tcgcccgtga agatatgggg
     2521 ctaacacttc ctcatgaaca tctgttcaac gatctttcct ctgttgtgga tgctccttgc
     2581 tatccttttt cacaacagct tgttgataaa aaagtcacgg cagaaatcca gtgggcatta
     2641 aaacacgatc cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc
     2701 ttcgaaatta ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct
     2761 gagtctatcg gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat
     2821 attgttgctt cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg
     2881 gttgatgaac tagcgacaac gatcgataaa gaattgaatc aagggattgg cgatacggat
     2941 attcgtgccg gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat
     3001 aacagcttgc gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt
     3061 cacatgccgg gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg
     3121 ggcgtctcgc caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg
     3181 tatcagcgga aaatgcttga taaaggtgtc tggctggaat tcgacatgat tggcctagac
     3241 attaccttcc cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct
     3301 catctcattg agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa
     3361 caaatgtggg ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc
     3421 tatctggcgg agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc
     3481 ggacgattat tgaccgcata aaaccattat cccccggcac attgcgccgg gggatgtcaa
     3541 ttaataccct gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc
     3601 atccgaccca accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg
     3661 accttttgct tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg
     3721 tccggcaacc actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca
     3781 caattgatgc tgttcaccac catttgcggc acggtagtga tgatccagct accggtaaca
     3841 aagtgagtac ataaaggcca tactgtcacg aacagtgcaa aagtggctct tcctcaacct
     3901 aggtgaagat tttcatgcca gtggactcat taccctccgc ctaagtagtt caagtccggc
     3961 tcgaccatac atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc
     4021 agtactccag cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc
     4081 agctacgcac cgaagttctg ctgagccgct ttcgtggacc cgagtgaacc agctgcttag
     4141 ctgtgattta ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgcgctat
     4201 tttcaactcc ggttcttttt cacacatcag actaataaat cggaacgcat aattttcttc
     4261 acccctgatt attctccagg gcatcaacca gcggcttacc cgggacactg aaggaagtct
     4321 tgcggcagta gtaaccggtg ggatccagcc tttatgccat ttggcaacag catccctgac
     4381 gatggtttca ctgcctgtaa cccccctggc caccatttcc cgccatatcc ggccggagtt
     4441 ataattacca cattctcgtt gctcttttaa ccactccctc catgggtcca gaagccctcg
     4501 ctttggaggc ctagttgaca tttcagggaa tgttcctgaa cccacccagc gactgactgt
     4561 cactcgcgac aggcctgtaa tacgggatat ttccctgaaa ctacatccct tgttatgcag
     4621 ggccataacc tctgtccaac gctgatgccg ttttttgcgg atgtgctgtt taaggcgttc
     4681 cagacgacgg agcgatgcta caggcacaga tatttctggc tcaggtgatt tcttcagtga
     4741 taactctctg acaacaagac gtatcagagg catatatctg tacatcattc gttcaggctc
     4801 atcaccaata ttttttagca ggtgccatcg atcggccacc tgtctggcct gaggtgcacc
     4861 ttcacgtgct gctgtcgcat agactccact gcgatcacgc gagacaacct gtatttccgg
     4921 atattttctg aaccaggtcg ccagcgtacg ctgatcacgg ccgggaagca ggacgagggg
     4981 acggtgagta tcaggattga ctattaacat accgcagcag tggccccgat gccacgccca
     5041 ctcatcaata ccgacatgag gcgcgcctga ctgtttcgtc tccggggtat tgataagcct
     5101 gcgaagaaga gtatcagcac tgcaacggag acccactgcc gttgcagccc gttttccagc
     5161 ctcacctcca gctattaatc ccagttgacg ttgttaattt tgtaacgcct gtgaagactg
     5221 ctggtgtgaa ctggcgaagg gagcaagcga ctcggcaaaa attttacgtg aacaagccgg
     5281 gttacggcag taccagtggc ggacggaaaa tacaagccat agcgtctgcc ctgagcaggg
     5341 tagatgctgt atccggcgcc gacgacaact atgaacagag tcgctacgct taaagcattc
     5401 aggacattgt gctgttttgc gttttccgtg gagatggagg gttataccgt cagagctaat
     5461 aatttgtcga ctgcatcgcc atccgcaagg taactgtaga agtgtcttaa gagagtgcat
     5521 tgcgttgccc ataacttaaa acagcttaga tccttgttcg ccagaaagtt tcatccattt
     5581 tcttccaccc aggttgcgga agaaccactt ttcatccgtc gttgacagat gtgaagtatg
     5641 cgggagaaaa agtgaatatc taactgtaat aactcagacc ggtacgagta ctcgggacgt
     5701 ctacaatcat tcagggcaaa gtgctaaaat ttctctatcc cgggatgccc ctaccaacgc
     5761 agcactcccg gagatatcat tacagctctg caactgattt ccgcgactga accgctcagt
     5821 tcctatcctt ccagttgccc tcctgccccc cctccctgct gtataccccg gtaccacaac
     5881 tcgggcagca cttttccccc tcataatttt cgtcacacat cacacaaaac cactttctca
     5941 ccttcaccgt ataatcc
//
LOCUS       DAZFWP020000228         5933 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFWP020000228 DAZFWP020000000
VERSION     DAZFWP020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.230"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(297..773)
                     /locus_tag="ACHK0Z_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             complement(297..773)
                     /locus_tag="ACHK0Z_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU4504561.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(832..2121)
                     /gene="bioA"
                     /locus_tag="ACHK0Z_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             complement(832..2121)
                     /gene="bioA"
                     /locus_tag="ACHK0Z_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU4504562.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQNRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2208..3248
                     /gene="bioB"
                     /locus_tag="ACHK0Z_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     CDS             2208..3248
                     /gene="bioB"
                     /locus_tag="ACHK0Z_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU4504563.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3245..4399
                     /gene="bioF"
                     /locus_tag="ACHK0Z_RS23435"
                     /old_locus_tag="pgaptmp_004687"
     CDS             3245..4399
                     /gene="bioF"
                     /locus_tag="ACHK0Z_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU4504564.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            4386..5141
                     /gene="bioC"
                     /locus_tag="ACHK0Z_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     CDS             4386..5141
                     /gene="bioC"
                     /locus_tag="ACHK0Z_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU4504565.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            5134..5811
                     /gene="bioD"
                     /locus_tag="ACHK0Z_RS23445"
                     /old_locus_tag="pgaptmp_004689"
     CDS             5134..5811
                     /gene="bioD"
                     /locus_tag="ACHK0Z_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4504566.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1405 a         1570 c         1646 g         1312 t
ORIGIN      
        1 tgacaaaaaa ttagcgcaag aggacaaaaa atcaccttgc gctaatgctc tgtctcaggt
       61 cactaatact atctaaatag ttgattcata gtgactgggt atgttgtgtt ttgtcgtatt
      121 ttgtagtcta ttatttagac tgagtcaatt ataagacgtt gatattaatg gtttttactg
      181 tttcacattc aactttttta tactaacttg agcgaaacgg gaaggtaaaa agacaaaaag
      241 ttgtttttaa tacctttaag tgataccaga tggcattgcg ccatctggca gagtgattaa
      301 ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga aatgaacgtt aaacccgacc
      361 atcgcgccgc tggcaccttc atcgacatca atacgttcta catccagcgc gtgaacggta
      421 aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc catcgtatcc ggttttacca
      481 aaatcggtac gcgtctgcaa aacgccgtct ggcattgcta ccagaccaga gccaaaccct
      541 tgcggtaata cgcgtgtatc agcgggtaag ttaacaacta cccagtgcca ccagccggag
      601 ccggttggcg catccgggtc gtagcaggtg acaacaaaac ttttcgttcc cgcaggaact
      661 tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt aacccatgcc gttaaagaca
      721 tgacgatgcg gcaacttatc gccatcgcgc agatcgttac tgatgagttt catgaaccct
      781 cctttcttgt ttgcagaaag tgtagccaga aaccctcacg ctgacttccc gttattggca
      841 aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg gtcagacgct gcaactgttg
      901 cgggagaata atatagggcg gcatcaggta aatcagtttg ccaaaaggcc ggatccagac
      961 accctgttcg acaaagaatt tttgcagcgc cgccatattc accggacgag tggtttcgac
     1021 cacaccgatt gcccccagta cgcgcacatc ggcaaccatt tcggcatcac gggcgggggc
     1081 aagttgctcg cgcagctgta cttcaatagc cgccacctgc tgctgccatt cgccggattc
     1141 gataatcgcc aggctggcgt ttgctgccgc gcaggccagc ggattgccca taaaagttgg
     1201 cccgtgcata aagcaaccgg cttcgccgtt actgatggtt tccgcaacct cgcgcgtggt
     1261 gagcgtggcg gaaagggtca ttgtgccgcc ggttaaggct ttaccgaggc acaaaatgtc
     1321 cggcgcgatt tctgcatatt cacaggcaaa cagtttgccg gtacgaccaa atccggtggc
     1381 gatctcgtcg gcaatcagca agataccttc gcgatcgcac attttgcgga ttcgttttaa
     1441 ccattccgga tggtacatgc gcatcccgcc tgcgccctgg acaatcggct caatgatcac
     1501 cgctgcgatt tcatgacgat gcgccgccat caggcgggca aaaccaacca tatcacgctc
     1561 atcccattca ccatccatgc ggttttgcgg ggcgggagca aacaagtttt ctggcagata
     1621 gcctttccac aggctgtgca ttgagttgtc cggatcgcac accgacatcg cgccaaaggt
     1681 atcgccatga taaccattgc ggaaggtcag aaaacgctgg cgcgcttcgc ctttggcttg
     1741 ccagtactgc aacgccattt tcatcgccac ttccaccgct acggaaccgg agtccgcgag
     1801 aaaaacgcac tccagtggtt gcggcgtcat cgccaccagt ttgcggcaca gctcaatggc
     1861 tggcgcatga gtgataccgc caaacatcac atgcgacatg gcatcaattt gcgtcttcat
     1921 cgccgcatta agctgcgggt gattgtagcc gtggatcgcc gcccaccagg acgacatacc
     1981 gtcaaccagg cgtctgccgt cagacaaaat tagctcgcaa ccttcggcgc tcgccaccgg
     2041 ataaaccggc agaggggagg tcatggatgt gtatgggtgc cagatatggc gttggtcaaa
     2101 ggcaatatcg tccgttgtca taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac
     2161 gagtctacac cgaattaaca acaaaaaaca cgttttggag aagccccatg gctcaccgcc
     2221 cacgctggac attgtcgcaa gtcacagaat tatttgaaaa accgttgctg gatctgctgt
     2281 ttgaagcgca gcaggtgcat cgacagcatt tcgatcctcg tcaggtgcag gtcagcactt
     2341 tgctgtcgat taagaccgga gcttgtccgg aagattgcaa atactgcccg caaagctcgc
     2401 gctacaaaac cgggctggaa gccgagcggt tgatggaagt tgaacaggtg ctggagtcgg
     2461 cgcgcaaagc gaaagctgcg ggatcgacgc gtttctgtat gggcgcggcg tggaagaatc
     2521 cccacgaacg cgatatgccg tacctggaac aaatggtgca gggggtaaaa gcgatggggc
     2581 tggaggcgtg tatgacgctg ggcacgttga gtgaatctca ggcgcagcgc cttgcgaatg
     2641 ccgggctgga ttactacaac cacaacctgg acacctcgcc ggagttttac ggcaatatca
     2701 tcaccacccg cacttatcag gaacgcctcg atacgctgga aaaagtgcgc gatgccggga
     2761 tcaaagtctg ttctggcggc attgtgggct taggcgaaac ggtaaaagat cgcgccggat
     2821 tattgctgca actggcaaac ctgccgacgc cgccggaaag cgtaccaatc aacatgctgg
     2881 tgaaggtgaa aggcacgccg cttgccgata acgatgatgt cgatgccttt gattttattc
     2941 gcaccattgc ggtcgcgcgg atcatgatgc caacctctta cgtgcgcctt tctgccggac
     3001 gcgagcagat gaacgaacag actcaggcga tgtgctttat ggcaggcgca aactcgattt
     3061 tctacggttg caaactgctg accacgccga atccggaaga agataaagac ctgcaactgt
     3121 tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct ggcaggggat aacgaacaac
     3181 agcaacgtct tgaacaggcg ctgatgaccc cggacaccga cgaatattac aacgcggcag
     3241 cattatgagc tggcaggaga aaatcaacgc ggcgctcgat gcgcggcgtg ctgccgatgc
     3301 cctgcgtcgc cgttatccag tggcgcaagg agccggacgc tggctggtgg cggatgatcg
     3361 ccagtatctg aacttttcca gtaacgatta tttaggttta agccatcatc cgcaaattat
     3421 ccgtgcctgg cagcagggtg cggagcaatt tggcgtcggt agcggcggtt ccggtcacgt
     3481 cagcggttat agcgttgcgc atcaggcgct ggaagaagaa ctggccgagt ggctgggcta
     3541 ttcgcgggca ctgctattta tctctggttt tgccgctaat caggcagtta ttgccgcgat
     3601 gatggcgaaa gaggaccgta ttgctgccga ccggcttagc catgcctcat tgctggaagc
     3661 tgccagttta agcccgtcgc agcttcgccg ttttgctcat aacgatgtca ctcatttggc
     3721 gcgattgctt gcttccccct gtccggggca gcaaatggtg gtgacagaag gcgtgttcag
     3781 catggacggc gatagtgcgc cactggcgga aatccagcag gtaacgcaac agcacaatgg
     3841 ctggttgatg gtcgatgatg cccacggcac gggcgttatc ggggagcagg ggcgcggcag
     3901 ctgctggctg caaaaggtaa aaccagaatt gctggtagtg acttttggca aaggatttgg
     3961 cgtcagcggg gcagcggtgc tttgctccag tacggtggcg gattatctgc tgcaattcgc
     4021 ccgccacctt atctacagca ccagtatgcc gcccgctcag gcgcaggcat tacgtgcgtc
     4081 gctggcggtc attcgcagtg aagaggggga cgctcggcgc gaaaaactgg cggcactcat
     4141 tacgcgtttt cgtgccggag tgcagggttc gccgtttacg cttgcggatt cacgtagcgc
     4201 catccagcca ttgatcgtcg gtgataacag ccgtgcgtta cagctggcag aaaaactgcg
     4261 ccagcaaggt tgctgggtga cggcgattcg cccgccaacc gtacccgctg gcaccgcgcg
     4321 gctacgcctg accttaaccg ccgcccatga aatgcaggat attgaccgtt tgctggagat
     4381 gctgcatggc aacggttaat aaacaagcca ttgcagcggc atttggtcgg gcagccgcgc
     4441 actatgagca acacgcagat ctacagcgcc agagtgctga cgtcttactg gcaatgctcc
     4501 cacagcgtaa atacacccac gtactggacg cggggtgtgg gccaggctgg atgagccgcc
     4561 actggcgaga gcgtcacgcg caggtgacgg cgttagatct ctcgccgcca atgcttgttc
     4621 aggcacgcca gaaggatgcc gcagaccatt atctggcggg agatatcgaa tccctgccgt
     4681 tagcgactgc gacgttcgat cttgcatgga gcaatctcgc ggtgcagtgg tgcggtaatt
     4741 tatccacggc actccgcgag ctgtatcggg tggtgcgccc cggaggcgtg gtcgcgttta
     4801 ccacgctggt gcagggatcg ttacccgaac tgcatcaggc gtggcaggca gtggacgagc
     4861 gtccgcatgc taatcgcttt ttaccaccag gtgcaatcga acagtcactg aacggcgtgc
     4921 attatcaaca tcatattcag cccatcacgc tgtggtttga tgatgcgctc agtgccatgc
     4981 gttcgctgaa aggcattgga gccacgcatc ttcatgaagg gcgcgacccg cgaatattaa
     5041 cgcgttcgca gttgcagcga ttgcaactgg cctggccaca aaaacagggg cgatatcccc
     5101 tgacgtatca tctttttttg ggagtgattg ctcgtgagta aacgttattt tgtcaccgga
     5161 acggataccg aagtggggaa aactgtcgcc agttgtgcac ttttacaagc cgcaaaggca
     5221 gtaggttatc ggacggcagg ttataaaccg gtcgcctctg gcagcgaaaa gaccccggaa
     5281 ggcttacgta atagtgacgc gctggcgtta cagcacaaca gcagcctgca gctggattac
     5341 gcaacggtaa atccttacac cttcgcagaa cccacctcgc cgcacatcat cagcgcgcaa
     5401 gagggcaggc cgatagaatc attggtaatg agcgccggat tacgcgcgct tgaacaacag
     5461 gctgactggg tgttagtgga aggtgctggc ggctggttta cgccgctttc tgacactttc
     5521 acttttgcag attgggtaac acaggaacaa ctgccggtga tactggtagt tggtgtgaaa
     5581 ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa tacaacacgc cggactgact
     5641 ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa aacgtcacgc tgaatatatg
     5701 accacgctca cccgcatgat tcccgcgccg ctgctgggag agatcccctg gcttgcagaa
     5761 aatccagaaa atgcggcaac cggaaagtac ataaaccttg ccttgttgta gccatcctgt
     5821 atttggttaa attgcgagcg agatcgcgtc ttcgattgac tgcaatttaa ccaattaaat
     5881 tctaaaataa tcacgaaaaa aattttactt ccgcctcatg cggcgaatgt ggg
//
LOCUS       DAZFWP020000229         5921 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFWP020000229 DAZFWP020000000
VERSION     DAZFWP020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.231"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..1340
                     /gene="dgcT"
                     /locus_tag="ACHK0Z_RS23450"
                     /old_locus_tag="pgaptmp_004690"
     CDS             3..1340
                     /gene="dgcT"
                     /locus_tag="ACHK0Z_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4504567.1"
                     /translation="MSSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALF
                     FIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQ
                     NDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFWGPIVAHI
                     LSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVT
                     VIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAH
                     RDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTV
                     VNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIP
                     QKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            complement(1381..2543)
                     /locus_tag="ACHK0Z_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /pseudo
     CDS             complement(1381..2543)
                     /locus_tag="ACHK0Z_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            2610..3137
                     /locus_tag="ACHK0Z_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /pseudo
     CDS             2610..3137
                     /locus_tag="ACHK0Z_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            3134..4120
                     /gene="ycdU"
                     /locus_tag="ACHK0Z_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             3134..4120
                     /gene="ycdU"
                     /locus_tag="ACHK0Z_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU4504568.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            4487..5689
                     /locus_tag="ACHK0Z_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             4487..5689
                     /locus_tag="ACHK0Z_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4504569.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         1500 a         1218 c         1452 g         1751 t
ORIGIN      
        1 gaattagtag tactgtatta gtgagcttat tgtttgggct tgctttggtt ttggtgaata
       61 gttggtttaa tcagccaggc gttgaggaag ttgttccacg atcaacctat ctgatggtaa
      121 tgattgcttt attttttatc gatactgttg catttatttt tatgcagttg tatttcattt
      181 atgaccgtag gcaattttca aactgtatac ttagtttggc ttttctgagt tgtttgattt
      241 actttgttaa aaccgtcatt atcattcagc aaattattga ggggcgtttg acaagcagtg
      301 ttgtccaaaa tgacattgca atctattatt tgtttcgtca gatgagtttg tgcatattaa
      361 tatttctggc attggtgaat aaagttagtg aaaacacaaa acagcgcaat ttattttcca
      421 aaaaaatgac tttgtgcata agtttgtttt ttgttttttg ggggccgata gttgctcaca
      481 tactttctag tcattatgag agctacaatt tacacattgc cgagttaacc aatgaaaatg
      541 gtcaggtggt ttggaaagcc tcatatgtta ccataatgat tttcatgtgg ttaaccttgc
      601 tttcggttaa tctatacttt aatggtttac gctatgatat ctggaatgga gtgacggtga
      661 ttgctttttg tgccgtactg tacaatatct ctctgttatt tatgagtagg tatagcgtgt
      721 ccacttggta tattagtcgc actattgaag tagtcagtaa gttaaccgtt atggtaatat
      781 ttatgtgcca tatattcagt gcgctacgag taacaaagaa cattgcacat cgcgatccct
      841 taaccaatat atttaacaga aattattttt ttaatgaact gacagttcaa tcagcatcag
      901 ccaaaaaaac gccttattgc gtcatgatta tggatatcga ccacttcaaa aaagtcaacg
      961 acacctgggg gcatccggtt ggcgatcagg tgataaaaac agtggtgaat atcattggga
     1021 aaagcatacg accagatgac cttttagcgc gcgtcggcgg cgaagagttt ggcgtcttgc
     1081 tgacggacat cgatactgaa cgtgcgaaag ctttagcgga aaggattcgg gaaaatgttg
     1141 agcgtttaac tggcgataat cctgaatacg ctataccaca aaaagtgacg attagtattg
     1201 gcgctgttgt gactcaggaa aatgcgttaa acccgaatga gatctatcga ctggctgata
     1261 acgcacttta cgaggcgaaa gagaccgggc gtaataaggt ggttgtgagg gatgtggtga
     1321 atttttgtga gtcaccataa agcggcattt tgatcctacc cacgtaatat ggacacaggc
     1381 ctaagcgagg ttcttgtttt caaattgttc cggactgagg ccgccacacc aactgtgccg
     1441 ccgccaccga ttgtaatcac attcgatata attaaacacc gttgcccgca ttatttcccg
     1501 gctgataaag tgttctccat ggatacattc cactttcagc gaatgaaaga agctttccac
     1561 gcaggcatta tcgtagcagc aaccttttgc gctcatactt ccacgcagat tatgccgctt
     1621 cagttgcgcc tgataatctg ctgaacagta ctggcctcca cggtccgtgt gaacgataac
     1681 gttccggggc ctcttacgcc gccacagcgc catctgcagg gcatcgcagg ccagttgcgc
     1741 cgtcatgcgt ggcgacattg accagccaat aacggcacgt gaccacaggt caatgaccac
     1801 tgccagatac agtcagcctt catctgtacg taagtacgtg atgtctcctg cccacttctg
     1861 gttcgggcca ctggcgtaaa aatcctgctc caacagattt tctgacacag gcaggccgtg
     1921 tgcgcggtag ctgaccgggc tgaacttccg ggaggccttt gccctcagtc cctgacggcg
     1981 caggcttgcc gccacggttt ttacgttaaa ggggtacccc tgagcacgca gttcatccgt
     2041 caggcgtggg gcaccgtaac gctgttttga ccgggtaaaa gccgcgagga caacgctgtc
     2101 gcagtgttgg cggaactgct gacgcgtgct tatccttgtc cgccgctgac accacgtata
     2161 ccagccgctg cgggccaccc ggagcacgcg gcacattgct ttgatgctga actcagcctg
     2221 atgtttttca ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
     2281 tggaggatag ccagctcttc atcccgttct gccagctggc gtttgagacg tgcaatctcg
     2341 gtagacatct ccagttcacg ttcagaagac gtctgctgat tttgctgttt actgcgccag
     2401 ttgtagagct gtgattcata caggctgagt tcacgggctg cggcagtaac accgatgcgt
     2461 tcagcaagct tcagggcttc actgcgaaat tcaggcgaat gctgtttacg gggtttttta
     2521 ctggttgata ctgtttttgt catgtgagtc acctctgact gagagtttac tcacttagcc
     2581 gcgtgtccac tattgctggg taagatcagg cggttaaagg catcaaccgc gaacaactgc
     2641 agacgatgat tacggaatca ggcattgcgc tggatattag ctgtgataac acgccgcgcc
     2701 agcaggttat tgggggaacg caggctgcgt tgaatgaatt tgccacgctg ctgatggccg
     2761 ctggatacga accggttaaa ctcggtgtga gcggtgcctg gcacacgcgt ttgatggaag
     2821 acggcgttca ggcaatgcgc gattatcttg ccggactgga tatcgcttcg ccagagcatc
     2881 aggttctgat gaacgtaaca gcaaaaagcg aggtcgctcc gtcaattatc aaagaaaatc
     2941 tctcattaca cttaactcat acggtgaaat ggactgaatc tttcgataca tttctgaata
     3001 tgccaacccc ggtagctttc cttgaaataa gtaataagcc ctatttgggg aatatgttaa
     3061 atgattttgc tggcgtggtt cagcaacgag ttatgcattg tcgtaaagca tttagtgatg
     3121 caaaggtatt caaatgattc ctgattattt aacttttatt cgctttcagg ataaacgaaa
     3181 tctgatatac atttatgcta ttggacttat tctgataggc ttttattgga agaatgcagg
     3241 gtttactttt ccatcagagg atattggtgt agttagtggg attctggccc tggtgctgta
     3301 taattttatt tttgatctca aggcgtactg ggcttataaa tgcgtcacga agaatatcga
     3361 tttttcgtgg tttaagaaaa agcagaacca caaaatagaa ttatttctta cacaacctct
     3421 ggtggcagga tttctgtcgt taatcatgtt gagtgcaatg agttgggggc tataccagct
     3481 tctaccctcg ttatatgcgc tgttcctgat ttcgttactt gggccgttgg tcatctttct
     3541 gctgtttcgg atgatccgca ccagttatgt caagcaggtc gctatttcag tagcgaaaaa
     3601 agtaaaatat aaaagtctga ctcgctatgt gctgctttcg gtgtgcatct caacggttgt
     3661 taacctgctt actatcagcc cgttgcgtaa cagtgattct tttgtgacag aggggcagtg
     3721 gttaacgttt aaatcgataa ttgcattgct cattctttgt ggcgtagtgt tggcgattaa
     3781 tctgtttttt ctgcgcttct ccaagcggta cgcttttctg ggcaggcttt ttttgcagga
     3841 aatcgatctg tttttctcca gtgaaaatgc gttgtcgacc ttttttgcca agccgctttg
     3901 gcttcggtta ttcatattgc tggttattga aatgatgtgg attacgctgg tgtcggtatt
     3961 ggcaacgctt gtggaatggc ggatttggtt tgaagcctat tttttactct gctatgtacc
     4021 gtgcttaatt tactattttt tccattgtcg attcctctgg cataacgatt ttatgatggc
     4081 atgtgacatg tatttccgtt gggggcattt taataagtga ggaagttaca ggaagtgacc
     4141 agataacgca tatatgttct ctactatctt gcgcattttg attgttgact gagtaaccag
     4201 acagttgatg tgcacaattt cccctcgccc taacagacgt gggcgggggc acccggtgtg
     4261 tgcaatatac gaaaaaaaag cccgtacttt cgtacgggct cttctttaaa tatggcggtg
     4321 agggggaccg cctgaaataa atctgactta atccaggggt gttatataat ttcattataa
     4381 acaatgtatt acactttttg ttgtccgata ttatctaatg tattcttagt gaagcgagag
     4441 actaatggca tactggatgg cataccagag tgacgggaat tagcgtatgg cagtattgac
     4501 ggatacgaaa gcaagacata tcaaacctga tgacaaacca ttgccccatg ggggaattac
     4561 cggactgacc cttcatcctt cttcagtaaa ggggcggggg aaatgggttt ttcgttatgt
     4621 aagtccggtg acacaaaaaa gacgtaatgc tggattggga acttacccag aggtcagtat
     4681 tgctgaagct gcacgtactg cccggataat gcgagagcaa cttgctgcag gtgatgatcc
     4741 tctggagatt aaaaaggctg aatctgagaa agtcgctatc ccaacatttg ccgatgcagc
     4801 caggcgtgta catgcagaac tgtctcctgg atgggaaaat ccaaagcatg taaggcagtg
     4861 gttatcgacg cttgagaatt acgcgtttcc tcaactggga gcaaaaacgc tggattcgat
     4921 tacggctgcg gacgtggcag aaacactgcg tccagtctgg ttaaccttgt cagaaacggc
     4981 aagccgggtt aaacagcgca ttcatgttgt tatgcagtgg ggatgggcgc acggtttttg
     5041 tgtagcaaat cctgttgatg tggttgacca tttgcttcct cagcagacaa gaggacgtga
     5101 tgaacaccaa cccgcaatgc cctggaggca gttaccgctt tttgtggcga ccagtgtgta
     5161 taccgatgaa ccttataatg ttacccgcgc actgttatta atggtgatac ttacagcaac
     5221 tcgctcgggc gaagccaggg gaatgcgctg ggctgaaatt gattttcata agcgggtatg
     5281 gactatacct gcagaaagaa tgaaagccag gatacagcat cgtgttcctt tatcccggca
     5341 ggctatttac attctggaaa atatacgtgg cctgcatgat gaactggtgt tcccttcacc
     5401 cagaaagcag cagatccttt ccgatatggt gttgacaagt tttctgcgta aaaagaaagc
     5461 cgtcagtgac attccggggc gagttgccac ggcacatggt tttcgctcaa cattcaggga
     5521 ctggtgtagc gaacaggggt attcgcggga tctggcggaa agggcgctcg ctcatacgct
     5581 gaaaaataag gttgaggcgg catatcatcg tactgatcta ctggagcagc gtgtaccgat
     5641 gatgcaggca tgggcggatt atgtgatgtc tcaaattgtg aataaataat agtgaaaatg
     5701 tatattgtac gatcatgatt tgatatgtga tcgtagcagt agtaattaag aaaccgcttt
     5761 tatgtcaaat gtattgagct gataattatc agatcgtcag gaaagcgtat tttgattaac
     5821 aggggattac ttaacaatcc cctttttata aaaaataatt tgcctgttta actcttcact
     5881 cctgctgaga ctgaattatt cctagtgcgt cataaatcag t
//
LOCUS       DAZFWP020000230         5918 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFWP020000230 DAZFWP020000000
VERSION     DAZFWP020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.232"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            218..352
                     /locus_tag="ACHK0Z_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             218..352
                     /locus_tag="ACHK0Z_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504570.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            956..1777
                     /locus_tag="ACHK0Z_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             956..1777
                     /locus_tag="ACHK0Z_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4504571.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1777..2883
                     /locus_tag="ACHK0Z_RS23485"
                     /old_locus_tag="pgaptmp_004697"
     CDS             1777..2883
                     /locus_tag="ACHK0Z_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4504572.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIECRFDINRTAQIIVSLASQAKGKCNR"
     gene            2973..4694
                     /gene="cptA"
                     /locus_tag="ACHK0Z_RS23490"
                     /old_locus_tag="pgaptmp_004698"
     CDS             2973..4694
                     /gene="cptA"
                     /locus_tag="ACHK0Z_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4504573.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETPRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4768..5766
                     /gene="lpxM"
                     /locus_tag="ACHK0Z_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /gene_synonym="msbB"
     CDS             4768..5766
                     /gene="lpxM"
                     /locus_tag="ACHK0Z_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4504574.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSAHQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
BASE COUNT         1523 a         1276 c         1481 g         1638 t
ORIGIN      
        1 tttacccatt gttttttcag ttcatgaatg tttgcaaaaa aaataaatga acaacaattt
       61 tatgtgttta aaataaatta attttacttc tgtaatggtg tcatgattag cagcttttta
      121 aactaatatc gactttcaat aaataaggac aggtaatgga caacaaaaat atctgaaccc
      181 gggtgagatt cataagttgc acagtgctgt ctttaaagtg ccacacccgg aacgtaatca
      241 ctgcctgctc cttatggggt atcttcatgg tgtccaagca tcagagttat taggtataaa
      301 actgtctgat attgatttgc aggccggtaa cctgaatatc cggcggctct aaacggtttg
      361 tcaactcagc ccctcggctg aaacaggagg tacagtccat tcgtcgctgg ctgaaacttc
      421 gggatgaatg ggtagaagag ggatatgaat ggttatttaa cctctatggc aggtctgcta
      481 caggtattac tgttgttctg ggggggggca ctggtgtggt taatatctac gatgcaagag
      541 attctcacga tggcccggtc catcagtgca tgcggcgcga tattgtgcga gtatttttca
      601 gggggagtgg tatccggatg cagaaaattc cgctactcga ctggttttat gcaataaatc
      661 agtatgataa tcttctgatt ttttactgag gcggatatct ctcgctgtgt ttactgaaat
      721 tgcggtattc tgttttttct gaaaaacaga aaatctgatt aaaaagagca tatgcgttaa
      781 gggaaattta agttctggtt gattaactga taaaaatatt aaaaaatccc tgttgtcggt
      841 aatctccgga ccattccggg attacacaat aatatcacca tgtgtgatat ctatggatcc
      901 tcactgttgt ttacgcgagg atattttcag aatgcttaac agacgataat tttttatgtt
      961 gaatgcaagg catcttcccg tactgatgta tcatcatgtc agtcgttgtc cggggcttgt
     1021 gacactctca cctgaaacat tttgtgaaca gatgaagtgg ctcgcagata ataactggcg
     1081 gacggtaaca tctgctgagc ttgaatattt ttatcagggg ggaacgcttc cacgaaagag
     1141 tgtcatgctc acattcgatg atggttatct tgataactgg ctcagagcat ggccggtcct
     1201 gaaggaatat aaccttcatg cacatatttt tctcattacg gggctgattg gtaagggaaa
     1261 cgtacgtagc aggcaggagc aggaatattc tcaccgcgac tgtgaacggt tgattgccga
     1321 taaccgttca gatgaggtca tgcttcgctg gtctgaagtc cgggaaatgc gtgattccgg
     1381 actggttgaa tttcatgtgc acacccattc acataaacgc tgggacaggt tgtctgtatc
     1441 acgtgctgaa cagtgccggc tcatgaaaga agatattctg gtgggaaaac aatgtctgac
     1501 ggaaaagctg gggttctgta gttcccatct gtgctggcct gaaggatatt ataatcggga
     1561 ttatattaat ctcgccggta agcttggttt ctcttattta tatacaaccg aaagaagaat
     1621 gaactgtcct gaaaatggtt cattacgcat agggcgtatc agcaccaaag agcgggaaca
     1681 ttccggctgg ctgaaacgac gcctttttta ttatacaacg ccactttttt cttcagtact
     1741 ggctcttcat aaggggccaa gattaccaga taattaatga tgaaaatttt attcactgaa
     1801 tcttcgtcag atattggggg ccaggagctg caggcactgg cccagatgac tgctcttcaa
     1861 aagcagggac attccgtttt acttgcctgc agggaaaaaa gtaaaattgc tccggaagcc
     1921 agaaaaagag ggcatgatgt cacttttatc cctttcagga acagccttca ccttccttcc
     1981 atcctcagac ttcgacggat tatcggggaa tttaaacctg atctggtcat ctgccatagt
     2041 ggccatgaca gtaatatagc aggactgagc cgtctgatat gctgtcaccg cttcagtatt
     2101 gtcagacaaa aaacctacat cacccgtaag acccgaacgt tttccctgaa ttatctgtgt
     2161 gatttcattg ttgtgcctag ctcagccatg atggcgcatc tgatggcgga aggtgtcagg
     2221 acaccggtaa cggttattcc accgggtttc gactggcccg cattgcataa tgaagccatg
     2281 cgaccgttac ccctgcatat ccacgcctgg gctgcatcag cagacaatgt tccgcttatc
     2341 gttcaggtgg ggatgctacg tcccgaaaaa ggacatgaat ttatgctgcg tgtattgtat
     2401 cagctgaaga tggaagggaa atcatttcgc tggctcgtgg tgggcgccgg gcgtgaagaa
     2461 tacgaagcac gtctgcgtca gcagacagaa cacctgggaa tgagcggaga tgtgttaatg
     2521 gcaggtgccc ttttcccagc gttaccggtc taccggattg ccagtgtggt ggtcatgcct
     2581 tcggaaaatg aagcgtttgg tatggtactg gctgaagcgt cagtctctgg cgtgcccgtt
     2641 atcgccagtg agacgggcgg gatccctgat gtcattcaga agaatgtgac agggacgcta
     2701 ttacccgtgg gggatgtgtc tgcatggacc ggcgcactgc gcgatttttt atcccgaccg
     2761 gaacgcttcc ggatgatggc agccagcgcc agagaagata ttgagtgccg gtttgatatt
     2821 aacagaacgg cacagatcat tgtgtctctg gctagtcagg ctaaggggaa atgtaacaga
     2881 taacggcgga tcctcttttc agcccgaatt ttgttcagcg gttttccggt aacggagtaa
     2941 tttatcacat tgtttaaata cggggacggt caatgcattt aaataccgga cagaacaggc
     3001 ctactttcag ctggagtgcg ctgggatggg caatttttta ttttggcttt ttttccactc
     3061 tcctgcaggt catcatcttc agcagtgggt acagcggaac gaatggaata cgggactcac
     3121 tgttattcag ttgtctgtgg ttgatcccgg tgtttctcta tcctgatcgg ataaaaataa
     3181 ttgcagctgt tgtcggtttc attctctggg gcacgtcgct ggcagcactt tgttattatt
     3241 ttctctatgg tcatgaattc tctcaaagtg ttcttttcgt tatgtttgaa acgaatgcca
     3301 gagaggctgg tgaatatttc agccagtatt tcagccttaa actgttgctt atctcgctgg
     3361 tatatactgc ggtgtccgtt tttctgtgga cacgtctgcg tcctgtatat attcctttgc
     3421 catggcggag aattgtctct ttcctgctgc tttatgctct gcttctgcat ccggttgttc
     3481 tgaaatcgtt aatcagacag gagccgctga atgatactct aggcaaactg gcatcccgaa
     3541 tggagcctgc tgccccctgg cagtttgtat ccagttatta tcagtaccat cagcaactga
     3601 atgcactgac aaccttcctg aatgaaaata gcgcactgcc accactgggt aatctcaggg
     3661 atgaatcagg ggagagacca cgcacactgg tcctggtgat tggtgagtcg acacagcgcg
     3721 aacgcatgag cttatacggg tatctacgtg aaacgacgcc ggagctggat gcactgcgta
     3781 aaaccgatcc gggtcttact gtgtttaata atgtggtggc atcgcgtccg tacaccattg
     3841 aagcattgca acaggccctt actttcgcca acgaaaagaa tcctgatctg tatctgacgc
     3901 agccgtcgct gatgaacatg atgaagcagg caggctataa aacattctgg attaccaacc
     3961 agcagacaat cacagcccgt aacaccatgc tcactgtatt ttcgcgccag acggacaggc
     4021 agtactacat gaatcagcag cgaacacaaa gtgcgcgtga atatgacact aacgtgctga
     4081 agccgttccg ggaagtgctg aatgaccctg caccaaagaa actgatcatc gtgcatctgc
     4141 tgggtacgca cattaagtat aaataccgtt atccggaagg tcaggggcgg tttgatggca
     4201 ttacagggca tattcccact ggattaaatg cgaaagagct ggaagtgtat aacgattacg
     4261 ataatgccaa tctgtttaac gatcatgtgg tggccagtct gataaaggac ttcagagcaa
     4321 ctgcgccgga cggctttctg ctttattttt cagatcatgg tgaagaagta tatgatactc
     4381 cgccatataa aacgcaggga cggaatgaag ataaccccac acgtcccatg tacactgttc
     4441 cgttcctgct gtggacctcg gaaaagtggc atgctgcaca tccgcgagat ttttcgcagt
     4501 atgttgaccg caaatacagt ctggctgaac tgatccacac ctggtcagat ttggcgggac
     4561 tgacatacga tggttacgat ccgacccgtt ctctggtgaa tccgcaattc agggaaaccc
     4621 cccgctggat tggaaatccg tataaaaaga atgggctcac tgatttcgac actcttccgt
     4681 atggtgagcc atagtatttt cttatcttcg gttccgttct gaaggcagga ggatgtgttt
     4741 tcctctgctg tccggtggcc tccatggatg gtaccacctt ctgctgtact ctgttatcat
     4801 aatgagataa gcaggcagat accggtaaat atgaaaaata tcagaactga gtttattccc
     4861 cgctttaacc ttacattatg cttcccccgt tactggatga cgtggacagg tatcggtatt
     4921 atctgtgtgt ttgcaatggt cccccccgca ctgagagatc cattgttggg aaaattgggc
     4981 atgctggttg ggcgtctggg gaaaagcgca catcaacggg ccttgattaa tttatcgctt
     5041 tgttttccgg agtacagtga taaagaaaag gagaatatcg ttgatgccat gtttgcaaca
     5101 gcctccatgg ccgttgtgct tatggctgaa ttagcactgt ccgggcctga taaaatttcg
     5161 catcgtatac gctggaatgg tctggagatt gtggagaaga tggcacagaa caatgaaaag
     5221 gtcatttttc ttgtgccaca tgcctgggga gtggatattc ccgcaatgct gatggctgcg
     5281 tcgggcagaa aaatggcagc gatgtttcat aaccagagaa atccggtggt ggattatgtc
     5341 tggaacagtg ttcgtcgtcg tttcggagga aaactgcacg cacgtaatga cggtattgcc
     5401 tcctttgtcc gctcagtccg gcagggatac tggggatatt atcttcctga tcaggatcat
     5461 ggcccggaat tcagtgagtt tgcagatttt tttgcgacgt ataaagcaac tttaccggtt
     5521 atcggacggc tcagcaggat cagtggtgca cggataatac cgcttttccc tgtttatgac
     5581 ggaaagaccc atcatctgac tattcatgtc agcccgccac ttgctatacg acagaagagt
     5641 gatgctcata ttgcgcgaca gataaacgaa gtggttgaaa atttcgttcg tccacatcct
     5701 gagcagtata cgtggatcct caagttgctg aaaacgcgta aagaagggga agaggatcct
     5761 tactgaggca ggtttttcag gataatgagc ttacccagca atagtggaca cacgctaagt
     5821 gagtaaactc tcagtcagag gtggctcata tgacaaaagt aagcgtacag cctgaaccgt
     5881 ctggtcagaa tctgacgaat tagacaaagt ggtgtcca
//
LOCUS       DAZFWP020000231         5860 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFWP020000231 DAZFWP020000000
VERSION     DAZFWP020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.233"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1918)
                     /gene="ehxB"
                     /locus_tag="ACHK0Z_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /gene_synonym="hlyB"
     CDS             complement(<1..1918)
                     /gene="ehxB"
                     /locus_tag="ACHK0Z_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGU4504575.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYE"
     gene            complement(1968..4964)
                     /gene="ehxA"
                     /locus_tag="ACHK0Z_RS23505"
                     /old_locus_tag="pgaptmp_004701"
     CDS             complement(1968..4964)
                     /gene="ehxA"
                     /locus_tag="ACHK0Z_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU4504576.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            complement(4966..5481)
                     /gene="ehxC"
                     /locus_tag="ACHK0Z_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /gene_synonym="hlyC"
     CDS             complement(4966..5481)
                     /gene="ehxC"
                     /locus_tag="ACHK0Z_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HGU4504577.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
BASE COUNT         1797 a         1317 c          910 g         1836 t
ORIGIN      
        1 actcataatc aagagcactg gttgcttcat caaaaataag aattcgagga tttgtaacaa
       61 gagcccgggc tattgcgatc cgttggcgct gccctcctga aagaccaacg ccctgttctc
      121 caacaatagt attgtaccct tcttttagtt ctgtaataaa atcatgggca cctgcaagtc
      181 tggctgcctc aatagcctgt tccatagata cagccggaga agctaatgta atattatcga
      241 taatactacg atttaatagt atattttcct gcaatacaac accaatctgg cgtcgtaacc
      301 attctggatc tgcaagtgat aaatcatgcc catcaattaa aatctgtccg gtctctggta
      361 tataaaagcg ctgaagcagt tttgttaatg tactttttcc tgaaccagaa cggccaacta
      421 taccgataac atcccccttt gatatgtata aattaatatt attcaaaata acattaccgt
      481 cagaagaata ccgaaatctg acatttttaa attcgatatc cccctgaatt tccggcagta
      541 tatttctgcc tgactttttc tctaccggtg tatttaatac atcaccaagg cgctcaacag
      601 aaataccaac ctgctgaaaa tcctgccaaa gatgagccag tctgataaca ggagcaatga
      661 cctgacctgc aagcatatta aacgcgatta actgcccgat gcttatctct cccgatatga
      721 caagatgtgc tcccatccat agactaatta ccattacact tttctgtatc agctgtatcc
      781 cctgctgccc tgtcattgca accagattta ccctgaaact ggaagcaaca tacccggcca
      841 gttgtttatc ccatgtagca atcatctgtg gtgatactgc catggattta attgtattta
      901 ttgctgttac cgtttcgaca agaaaagctt gattttctgc attcctgaga aacttatcat
      961 caagacgtcg acgtaataag ggtgatataa acaatgacca tataacataa caaggtagtg
     1021 acaataatat aaccagtgtt aattgagggc tgtaatacca catgacacaa aaaaatatta
     1081 cagaaaaaaa tagatcaaga actgatgtca acgcttgtcc ggttaaaaaa ttacgaattt
     1141 gctccagttc ccttactctg gcaacggtct ctccgaccct gcgattttca aaatatgaaa
     1201 caggcaatgc aagcaaatgt ctgaataact tagcgctcag ttcgacgtca atacgacttg
     1261 tagtatgaga gaaaatataa gttctggctc cggttaatat cacttcaaaa agtatcacta
     1321 taataaatgc tattgtgata atatttaacg ttgaaaaccc ccggtgaact aaaaccttat
     1381 ccattacaac ctgaaaaaaa agaggtgtta ttaacgcaag aaactgaata aaagcagaaa
     1441 cagttaacac ctcaagtaaa atcctcctgt atttcacaac agaggggata aaccaagaaa
     1501 aatcaaattt agctaactct ccgactactg ttgctcttga cgtaaccaga atgacttttc
     1561 cctgatacag tttttcaaac tcatcccgac taaaagttag tgactgatgt tgttctggat
     1621 cataaacaag atagcgtgat gaatctttag taacacgaga caatatataa tgcttaccgt
     1681 catcccgcca tatcaacgca ggtaaagaaa ttattgacaa tctggaaaaa tgtttttcta
     1741 catattttgc ttttaagcca atggatttcg ctgccagtaa ccattcagtc accccaaaat
     1801 cttgtgtgtg ggtattatac tgatgcctta tagtttcagc attgacagtt atattatgat
     1861 attgtgcaag caatatcagt gcatacagac tattatgaga actacattta ctcatcatat
     1921 aacgatgacc attcctcctg gaatggccat cacctcctct tttagtctca gacagttgtc
     1981 gttaaagttg ttgagtgtgt gttgttttga tgtaaggata aattatataa agatgctaat
     2041 cgttcatcac ctgaatcgaa tcctgaaact gaagaaataa tcttattaat atcattggca
     2101 acactggtga tattaaccat gtttttttca gatgctatat tactggcttt tatataacct
     2161 gaccgttcat tattattatg aggtatttta tcaactttta tctctcgacc atctttatct
     2221 gttataacct caacaagatg attatctgct cctgaggcat ctttcgcaaa ccagttttta
     2281 aatgttatcc cattgacatc atttgactca ttaaatgaaa gtacaccatt aatggcttta
     2341 ttcatgataa gatcatttcc aactctctta aatgcgatat catcaaagct aatatcagat
     2401 aagtgcaaac gatctccttt acctccttca tctgcaattg tatgatgacc atagttttgc
     2461 ctataaacat aaatatcatt accaaaacca ccattcagat agtcattacc ttcccctcca
     2521 tccagaagat caataccacc accaccataa agtttatcat caccagtacc acctgacaga
     2581 atattataag ctccgtgtgc ctgaagctca tcattaccgc ttcctcctgt cagataatta
     2641 ttaccatccc ctccactcaa tttatcgttt ccactaccac caaataactg gtcgtctccc
     2701 tgtcctccgg atatataatc atccccatca tcgccgtata gtcgatcatt accataatta
     2761 ccttcgatat aatcgttccc atctgcgcca tggaatatat cattaaactt accgcctttg
     2821 aattcatcat catgttttcc gccaatgagc tcttcaacag aatgaagatt atctattaca
     2881 tcataaggaa ttccaccggt tctgaattca aaatcacgat attgtatttt ctctgttcgc
     2941 ttccctactg acacctcctg ttccttaacg acttcctgca ataccttcac gtcaccatac
     3001 atattttttg aaactatata ctcaccaggt tttgatgctc ctgttgcatc aattgttagt
     3061 ttaccaacat ccgtcttatt ataagatacg gtatcatgac cttcaccagc aaatacttct
     3121 gatgagcctg ctccagaata aaccacatcg tctttatctc cgagctcaga aattattctc
     3181 gcctgatagt gtttgttatt cttttcaaca aactgaatca attttgaata atcatataca
     3241 cctttatgat tttttatgcc ttttacagac catgtatcct taccatttac aataagagat
     3301 gtcatatatt catatttacc actctgtttt ctttctctaa cttcttctcc tggggtaaat
     3361 gttggtgtta taaatgtcaa aacacttgat acattacctg ttgaaaggtc tatggtccct
     3421 ttttgaggat caaacacttg ttgtgtaaac tccttcggtt gagcctctaa aagccctcca
     3481 ttttccagat aattaatata tgccttacca ctctgactgc gatcagcatt acgggttatg
     3541 cctgcaagtt caccgatctt ctcatcccaa tgttgctggg ttattgcgac tgctctttct
     3601 actgcatgct gacgagaaaa atcagcaagc aaagacagag agtcttctaa aaacgcagca
     3661 tgtcttgcgt catagccatt ctcaaaataa tttttgccat gctccttttc ccattcattg
     3721 atccgagcag cgaatttctc tgcaacgtgc tcaaacatag cctgttttga tgcttccaga
     3781 atgccagata tcgtaccggt taatgcactc accagcatgc ttatcggggc ccctatgagg
     3841 gatgcactgg aggctgcact aactcccgca gatacagaac tcaggacagt atttattgtt
     3901 gtcagggctg catctatagc tccggtttct ttatgaaagg ctgcgagtaa agcatcccct
     3961 tcataattca attttttaaa tcgttcagaa tatgattcaa gctgcttagc tcgctcaaat
     4021 ttatctgcag cagccaggaa agaaagagga ctgatagcca gcataacagc cgatgtgatc
     4081 agacccgcac ttgcagctgt tgtcgataac ccctgtgcca ttctctgagc cagaatatat
     4141 tgcgaaacag ctttaccaac atttccaaga acctgagttg tcagttctat acccgctgca
     4201 gcttttgttc ctgtatgtgc gtcactgttt cccagaataa agcttgctga tacagcagaa
     4261 agaattccgg agacaacatc cagcccatcc cccagggagc ccaggtctgg taaattttgt
     4321 aatttcccac caacagaact taagcgaggt ttactggata aaaatgagcc aagctggtta
     4381 agctgctcag aaaatgaatc aacggtactg tttatacttg atactgtatc tacaagctga
     4441 ttaataagtt caatactact tttggcaata tcattctgac ttatatcctc tccttcccgt
     4501 tgttttctca gcaattcatc aagagccatg cctgataaag caatccccgt aaaattctgt
     4561 agtgctgaga gaactgttcc ggcttttccc agattattac ctacattttc agcggttcct
     4621 cctattttac tcccaacttt ctgatacttc tgcagaagtt tgtcaagttg tggtgcgaat
     4681 attgcaacac cacgttcagt taatcctaca actttttctg ctgcaccaaa aaactggttt
     4741 gcaatcgctg tatcatctcg ttcagtacgg tgtatttcta ttccaagctc atcagcagct
     4801 ttgaccaact cattaatacc cacgccctga gcttcataat tatcaggtat taataatatg
     4861 agttttttgc cagcggaacg tacgcttgat gatgctgtat taaatgctga ttttgtagtc
     4921 aatgtcgcat tattgaaaat gttctttatt ttatttactg tcatattagt tttctttctc
     4981 aatattaaaa ttaaaatctt gtctgttttt tacttcagac ataagttcat gatggtattg
     5041 cctgaatatt tttgatgcca gttttttatc tatcccccct ccatgaaatt cagctatttt
     5101 tcctgtttta ctgctctcat ctaaccttat tgctctgaat aactcataag gatacttttt
     5161 cctcatgtat ttatataact ccatgttgtg tccaaatgga gctatccagt caataaacca
     5221 ctttctttct cccgacatcc agtcttttgc atacaaagat gttacatcat ttatatattt
     5281 tacctcacat tccagatcta aactggccca gctacaaaaa gctacaggaa gctcatcctt
     5341 tattaagaga gcaaattgat ttgtctgaat agcaggtatg acattaatag caaaaacaga
     5401 taatggccat tttttatgca gaggagaaca ggcccatagc catgcaacct tccccatgac
     5461 gtcaaaagca ttagatttca taatgtttaa ataaataaga aaactcagta aatgtaaaga
     5521 atcctatata aaagaacaga aattatatct ttttaagcaa gaagcatcta aaaacaaaaa
     5581 gctaccgcaa ggagaatctc tcccaaccaa aacaacatta gcgataataa tatattgcct
     5641 aaacattctt acacatatca cagctaagtt atattataac actcagatga ttgatttgta
     5701 agataacgta tgattttcat atgaatagcc acgtgacaaa tgccttatta aagagattgt
     5761 aaacagaata cagcaaacta tttcagttac cgaaatgacg agttaatcag ttttgtttac
     5821 cctgcaaaca caaaatctaa acctacacag aaaagcaaac
//
LOCUS       DAZFWP020000232         5732 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFWP020000232 DAZFWP020000000
VERSION     DAZFWP020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.234"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="ACHK0Z_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="ACHK0Z_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4504578.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHK0Z_RS23520"
                     /old_locus_tag="pgaptmp_004704"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHK0Z_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU4504579.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /gene="ydbJ"
                     /locus_tag="ACHK0Z_RS23525"
                     /old_locus_tag="pgaptmp_004705"
     CDS             complement(1535..1801)
                     /gene="ydbJ"
                     /locus_tag="ACHK0Z_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU4504580.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHK0Z_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHK0Z_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU4504581.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKCQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1424 a         1476 c         1491 g         1341 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc
      301 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
      841 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     1081 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaac
     1201 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga acgtttgggg
     2221 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccgacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgtagcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg tcatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc caggaagcgc aagattttgc
     2521 tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcacg tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca ctattcttga
     2641 tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac gacgcggtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg
     2941 tggcgaaaaa gttggcgtgc tgaaagttcg cctgtaccgt cccttctccg ccaaacactt
     3001 gctgcaagct ctgccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtaatgacc gcactggcag aagcctttaa
     3121 taatggcgag cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtgaccaat ctgtcactgc cgttgccgga
     3301 aaacaccctg ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatgtca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaggaagt
     3661 tcaggccgtg ctaaatcaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcgcg
     3901 tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc
     3961 agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgcagcga tgctcgcagg
     4021 gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac
     4081 cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgcg ttgccgcttg cccacactca gctattcgcg caaaagtggt
     4201 gccgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attactgact cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc
     4621 aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc
     4681 ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     4801 ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt
     4861 cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tacgtgatgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggt gatggctggg cttacgatat
     4981 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     5101 agtaactaaa tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tcaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca tcgcttatag
     5281 cccatgtgaa gagcatggtt acgatctggc actcagccac gaccagatgc gccaactcac
     5341 agctaccggc ttctggccgc tatatcgctt cgatccgcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agtagcactg gaagaaacgt tacttcacga
     5461 gcaacgtttc cgtcggctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaagcccg
     5641 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
     5701 acagaaaata aaatctgtga caataaaggc at
//
LOCUS       DAZFWP020000233         5711 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFWP020000233 DAZFWP020000000
VERSION     DAZFWP020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.235"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            367..3216
                     /gene="cah"
                     /locus_tag="ACHK0Z_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             367..3216
                     /gene="cah"
                     /locus_tag="ACHK0Z_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HGU4504582.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            3327..5711
                     /locus_tag="ACHK0Z_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             3327..5711
                     /locus_tag="ACHK0Z_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGU4504583.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
BASE COUNT         1469 a         1429 c         1652 g         1161 t
ORIGIN      
        1 tctgccaggg aaatgcctga taacattacg ttaaccaact gaaaacatga cacaaacccc
       61 tcatactcct cctgtttctc ttgcgtgacc gccttgtcgt taatagaata aaacgatcga
      121 taaaacaggt atcgatcgtt gctatcgatc gtttatagtg gttaatggtc tgcgaaaagg
      181 cagctgaatc tcttcatcat gcagaacgga attgcacaca acagactgat acttctctgt
      241 ctgatgaagg gggagagcgt ttacgcccgg gacgtatcgc tgtgcccgat aactctgttt
      301 tgtacctgcc ggtatccact tttgtgggta ccggcttttt tattcaccct ctgtaaggaa
      361 aagctgatga aacgacatct gaacaccagc tacaggctgg tatggaatca cattacgggc
      421 accctggtgg tggcctccga actggcccgc tcacggggaa aacgcgccgg tgtggcggtt
      481 gcgctgtctc ttgctgctgt cacatcagtc ccggcactgg ctgctgacaa ggttgtacag
      541 gcgggagaaa ccgtgaacga tggaacactg acaaatcatg acaaccagat tgtcttcggt
      601 acggccaacg gaatgaccat cagtaccggg ctggaactgg ggccggacag tgaagaaaac
      661 accggtgggc aatggataca gaatggcggg atagccggaa acaccactgt caccacaaat
      721 ggtcgtcagg tcgtgctgga ggggggaaca gccagtgata cggttattcg tgacggcggg
      781 ggacagagcc tgaacggact ggcggtgaac accacactga ataacagagg cgagcagtgg
      841 gtgcatgagg gcggggttgc caccggtaca attatcaacc gcgacggtta ccagagcgtt
      901 aaaagtggcg ggctggcaac aggaaccatc atcaacaccg gcgcagaagg cggccctgat
      961 tctgacaact cgtatacggg tcagaaggtc cagggaacag cagaatccac caccatcaac
     1021 aaaaatggac ggcagattat cttattttcc gggctagccc gtgacactct catttacgca
     1081 ggtggtgacc agtcggtaca cggaagggcc ctgaatacca cactgaatgg cggttaccag
     1141 tatgtgcaca gggacggact tgcgctgaac acggtaatta acgagggggg ctggcaggtt
     1201 gttaaggcag gtggcgctgc cggtaacacc accataaatc agaacggtga actgagggta
     1261 catgccggcg gggaagccac tgcagtcacc cagaacacgg gcggtgcact ggttaccagt
     1321 actgctgcaa ctgtcatcgg cacaaaccgt ctggggaatt tcacggtgga aaacggtaag
     1381 gctgacggtg ttgttctgga atccggcggt cgtctggatg tactggagag ccattcagca
     1441 cagaataccc tagtggatga cggcggtacc ctggcagtgt ctgccggcgg taaggcgaca
     1501 agtgtcacca taacatccgg tggtgccctg attgcagaca gtggtgccac tgttgagggg
     1561 accaatgcca gcggtaagtt cagtattgat ggcacatccg gtcaggccag cggcctgctg
     1621 ctggaaaatg gcggcagctt tacggttaat gccgggggac aggctggcaa caccactgtc
     1681 ggacatcgtg gaacactgac gctggctgcc gggggaagtc tgagtggcag aacacagctc
     1741 agtaaaggcg ccagtatggt actgaatggt gatgtggtca gtaccggcga tattgttaac
     1801 gcaggggaga ttcgctttga taatcagacg acaccgaatg ccgcgctgag ccgtgctgtt
     1861 gcaaaaagta actccccggt aacgttccat aaactgacca ccacgaacct caccggccag
     1921 ggcggcacca tcaatatgcg tgttcgcctt gatggcagca atgcctctga ccagctggtg
     1981 attaatggtg gtcaggcaac cggcaaaacc tggcttgcgt ttacaaatgt cggaaacagc
     2041 aacctcgggg tggcaaccac cggacagggt atccgggttg tggatgcaca gaatggcgcc
     2101 accacagaag aaggtgcgtt tgccctgagt cgcccgcttc aggccggcgc ctttaactac
     2161 accctgaacc gtgacagcga tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct
     2221 gaagtccccc tgtatacatc catgttgaca caggcaatgg actatgaccg gattctggca
     2281 ggctcccgca gccatcagac cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt
     2341 cagggcggtc atctcggtca cgataacaac ggcggtattg cccgtggagc cacgccggaa
     2401 agcagcggca gctatggctt cgtccgtctg gagggtgacc tgctcagaac agaggttgcc
     2461 ggtatgtctc tgacgacagg ggtgtatggt gctgcaggcc attcttccgt tgatgttaag
     2521 gatgatgacg gttcccgcgc cggcacggtc cgggatgatg ccggcagtct gggcggatac
     2581 ctgaatctgg tacacacatc ctccggcctg tgggctgaca ttgtggccca gggaacccgt
     2641 cacagcatga aagcgtcatc ggacaataac gacttccgcg cccggggctg gggctggctg
     2701 ggctcactgg aaaccggtct gcccttcagt atcactgaca atctgatgct ggagccacaa
     2761 ctgcagtaca cctggcaggg actctccctg gatgacggcc aggataacgc cggttatgtg
     2821 aagttcgggc atggcagtgc acaacatgtg cgtgccggtt tccgtctggg cagccacaac
     2881 gatatgacct ttggtgaagg cacctcatcc cgtgacaccc tgcgcgacag tgcaaaacac
     2941 agtgtgagtg aactgccggt gaactggtgg gtacagcctt ctgttatccg caccttcagc
     3001 tcccggggtg acatgagcat ggggacagcc gcagccggca gtaacatgac gttctcaccg
     3061 tcccggaatg gcacgtcact ggacctgcag gccggactgg aagcccgtat ccgggaaaat
     3121 atcaccctgg gcgttcaggc cggttatgcc cacagcgtca gcggcagcag cgctgaaggc
     3181 tataacggtc aggctacgct gaatatgact ttctgattat acggtatccc ggaaatacga
     3241 tttcaggaaa attcatgcag taatgggaaa ggacgctcat tgctgttcac cgcagttctg
     3301 aattattgaa tttataaagg tgaatgatgc acgaaaagaa catcgccctg ctttgtgatg
     3361 aagccgaccg gcttttgcaa ctgaatatca atctgctccg gcaaatggtt gatgagccgg
     3421 atgtgttatc tgacagtaag aacgaaaacg ggctgctttt tgataaacgg aaagcgctga
     3481 aaagaattga ggagctggag ggcgaacaaa tcaaaaccgc ccgcagggag atggtgctgg
     3541 ctgtggtcgg cacgatgaag gcgggcaaat caaccaccat caacgccatt gtggggcagg
     3601 aaattctgcc caaccgtaac cgccccatga cctcggtacc gacattgatc cgccacgttc
     3661 ccggaaaaac ggaaccggtc ctccatctgg aacatattca gcccgtccgc aatttattaa
     3721 tcacactgca ggaaaaactc gccaccccgg cagggcagca agtcgcacag tccctgcagc
     3781 aaaccgggga tacccgcgaa ctactggata ttctggcgga tgatgtctgg cttaaaaatg
     3841 aataccacgg ggaagacgaa atctttaccg gactggcatc gttaaatgac ctggttcgtc
     3901 ttgctgcggc aatggggact gaatttcctt ttgacgaata cgcagaagtg cagaaactac
     3961 cggtgatcga cgtggcgttc agccacctgg tgggaatgga tgcatgtcag ggaacactca
     4021 cactgctgga tacccccggc cctaatgaag ccggacaacc acagatggaa atgatgatgc
     4081 gggatcaact acagaaagcc tctgcggtgc tggcggtgat ggattacacc cagatgaact
     4141 cgaaagccga tgaggaggtt cgtaaagagc ttaatgccat tgctgacgtc tcagccggtc
     4201 gtctgtttgt gctggtcaat aaattcgatg aaaaagatcg caatggtgat ggtgcggatg
     4261 ccgtacgcca gaaggtgcca gcaatgctga acagcgatgt gctgcccgcc tcccgcgttt
     4321 atcccggctc atcacgtcag gcgtacctgg ctaaccgtgc gcttcatgag ttacgaaaaa
     4381 acggtgcgct tccggttgat gaagcctggg tcgatgattt tatcagggaa gctttcggac
     4441 ctatggtgga agaggacgac tggaaagaca gcactaaggt aaataaaaag gcagaaaaac
     4501 tctggaatat atctctgatc gatcaactga taacggaagt catacagagc tcccattcca
     4561 gagcggcggc actggcggtt gactctgccg ccgcaaaact gatgcagaat gcagaaaatg
     4621 tcagcgaata tctgtcatta cgtcatcagg ggctgcagca aagcattcag tcactacagg
     4681 cgcatattac cagcctgctg gcggatatcc aggagattga ggaatgtcag aatcaggtga
     4741 ccggtgatgt cagaatggcc atggaggaca tcaataccaa aaccggggaa ttactgacga
     4801 aggtctgtgc ctcactggag gaggagctga acgactattt cagaagcggt aaacgcaaag
     4861 aacagcaaat gctggaggaa gaaaattcag cgcagccccg ggagagaaac gcattcgctt
     4921 tctttcacga tattttcgga accggaaatc agcatgacag gatgcgggac tttgatccgg
     4981 acagccccga aataaaattc agtgatcgca gggcagccct tgaactgatg acgcaaatcg
     5041 aatcgaccgt gaccagcctg caccgtgagg ctgaagcaca gttccggcct gagctggaga
     5101 aaatcgtcag cgggattgaa acaggttttc gtggcacggc cctgtacgcc acagaaaaca
     5161 ttgccggtcg catcaatgcc cgcctggagg atgagggctt taccgtaaaa atcagttttc
     5221 cggcagtcag ccagttacag acccggctcg cggtaaaaat aaatctgagt gcgcttatgg
     5281 aggaaagaac ggagaccgtc acccgtcgcc gtcggcagag tggcgtatgg ggaaccgttt
     5341 gtcgatggtt tggcaccagt gacctgggct gggaaaacta tgacgaggat gtgagtcgca
     5401 gcgtgatcaa tatcaacaag gtcagagagg aagttatgtc actgacccgg gcatatttcg
     5461 gggagctgca ggcatccatt gagcaggata ttaaccagcc cgtccgccag gaaatcgatg
     5521 cctttttctg cgcattcagg gagaaagttg aacaactgcg taacacgctg attcagagct
     5581 ctgaagatca taaacgcgat cagcaggcgc aggaacgact taccgggcga cttcaggcat
     5641 taaatgaaag ggttcctgag ctcattactg acagtaaggc gctgagggaa gagctggaga
     5701 caatgctgtg a
//
LOCUS       DAZFWP020000234         5700 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFWP020000234 DAZFWP020000000
VERSION     DAZFWP020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.236"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..623
                     /gene="fimA"
                     /locus_tag="ACHK0Z_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             81..623
                     /gene="fimA"
                     /locus_tag="ACHK0Z_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4504584.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            843..1535
                     /gene="fimC"
                     /locus_tag="ACHK0Z_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     CDS             843..1535
                     /gene="fimC"
                     /locus_tag="ACHK0Z_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4504585.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1566..4174
                     /locus_tag="ACHK0Z_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /pseudo
     CDS             1566..4174
                     /locus_tag="ACHK0Z_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4187..5194
                     /gene="fimH"
                     /locus_tag="ACHK0Z_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             4187..5194
                     /gene="fimH"
                     /locus_tag="ACHK0Z_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4504586.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5205..>5700
                     /gene="sfmF"
                     /locus_tag="ACHK0Z_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             5205..>5700
                     /gene="sfmF"
                     /locus_tag="ACHK0Z_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU4504587.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAI"
BASE COUNT         1495 a         1236 c         1373 g         1596 t
ORIGIN      
        1 tttactacta tcagtgtctt aaataaagta atcggttata tacggatgtg gagtcgataa
       61 atgagattga aggaatatat atgaaattaa gatttatttc gtctgcgctg gctgccgcac
      121 tattcgccgc tacgggtagt tatgctgccg ttgtagatgg cggtacaatt cactttgaag
      181 gcgaactggt gaatgctgcc tgttcagtga atactgactc ggcagaccag gttgtcacac
      241 tcggtcaata tcgtaccgat attttcaatg ctgttggtaa tacctctgca ttaattccat
      301 tcaccattca gttgaacgac tgcgatcctg ttgttgccgc taatgctgcc gttgcatttt
      361 ctggtcaggc tgatgcaatc aatgataatt tattggccat tgcatccagt accaatacaa
      421 caacagcaac gggtgtcggt attgaaatac ttgataatac atccgcaatt ctcaaacctg
      481 atgggaatag cttctcaacc aaccagaact tgatccccgg aaccaacgtt cttcattttt
      541 ctgcacgtta taaaggcacc ggtacaagtg catcagcagg gcaagcaaat gctgacgcga
      601 cttttattat gagatacgaa taatcaaaac cacgttgttt tgaattatat atcacgtctt
      661 ataacaaagt aatgtaccgg ttgtctgaag cggtatggtg gcaatgtaaa tcgaaatcat
      721 gttcactttg tatcatgccg ctttattaaa tgaaaaggga atgatgtgtt gtaagaaacc
      781 aaagcaatca tttctttata tttcttattt ttgccgtcag gaatacacaa ggcgtattaa
      841 ctatgatgac taaaataaag ttattgatgc tcattatatt ttatttaatc atttcggcca
      901 gcgcccatgc tgccggaggg atcgcattag gtgccacgcg tattatttat cccgctgatg
      961 ctaaacagac tgcggtatgg attagaaata gccataccaa tgagcgtttt ctggtcaatt
     1021 cgtggattga aaacagcagc ggtgtaaaag aaaagtcatt catcattaca ccgccactgt
     1081 ttgttagtga acccaaaagc gaaaatactt tgcgtattat ttacaccggt ccaccgctgg
     1141 cagcagatcg tgagtctctg ttctggatga atgttaagac gatcccttcg gtagataaaa
     1201 atgcattgaa cggcaggaat gttttgcaac tggcgatttt atcgcgcatg aaattatttc
     1261 tccgtccaat tcaattacaa gaattacccg cagaagcgcc ggacacactc aagttttcgc
     1321 gatccggtaa ctatatcaat gttcataatc catcaccttt ttatgtcacc ctcgttaacc
     1381 tgcaagtggg cagccaaaag ttggggaatg ctatggcggc acccagagtt agttcacaaa
     1441 ttcccttacc ctcaggagtg cagggaaagc tgaaatttca gaccgttaat gattatggtt
     1501 cagtaacccc ggtcagagaa gtgaacttaa actaaccgaa tcatctgaca atatcagagc
     1561 taattatgaa aatacccact actacggata ttccgcagag gtatacctgg tgtctggccg
     1621 gaatttgtta ttcatctctt gccattttac tctccttttt aagctatgcg gaaagttatt
     1681 tcaacccggc atttttatta gagaatggca catccgttgc tgatttatcg cgctttgaga
     1741 gaggcaatca tcaacctgcg ggagtgtatc gggtggatct ctggcgtaat gatgagttca
     1801 ttggttcaca ggatatcgta tttgaatcga caacagaaaa tacaggtgat aaatcaggtg
     1861 ggttaatgcc ctgttttaac caggtactcc ttgaacgaat tggccttaat agcagtgcat
     1921 ttcccgagtt agcccagcag caaaacaata aatgcatcaa tttactgaaa gctgtacctg
     1981 atgccacaat taactttgat tttgcagcga tgcgcctgaa catcactatt cctcagataa
     2041 cgttgttgag tagcgctcac ggttacattc cgcctgaaga gtgggatgaa ggtattcctg
     2101 ctttactcct gaattataat ttcaccggta acagaggtaa tggtaacgat agctattttt
     2161 ttagtgagct cagcgggatt aatattggcc cgtggcgttt acgcaacaat ggttcctgga
     2221 actattttcg cggaaatgga tatcattcag aacagtggaa taatattggc acctgggtac
     2281 agcgcgccat tattccgctg aaaagtgaac tggtaatggg agacggcaat acaggaagtg
     2341 atattttcga tggtgttgga tttcgtggtg tacggcttta ttcttccgat aatatgtatc
     2401 ctgatagcca gcaagggttt gccccaacgg tacgtgggat tgcccgtacg gcggcccagc
     2461 taacgattcg gcaaaatggt tttattatct atcaaagcta tgtttccccc ggcgcttttg
     2521 aaattacaga tttgcacccg acatcttcaa atggcgatct ggacgtcacc atcgacgagc
     2581 gcgatggtaa tcagcagaat tacactatcc cgtattcaac agtgccaatt ttacaacgcg
     2641 aagggcgttt caaatttgac ctgacggcgg gcgattttcg tagcggtaat agtcagcaat
     2701 catcgccttt cttttttcag ggcacggcac tcggcggttt accacaggaa tttactgcct
     2761 acggcgggac gcaattatct gccaattaca ccgccttttt attaggactg gggcgcaatc
     2821 tcggaaactg gggggcagtg tcgctggatg tgacccatgc gcgcagtcag ttagccgacg
     2881 acagtcgtca tgagggggat tccattcgct tcctctatgc gaaatcgatg aacaccttcg
     2941 gcaccaattt tcagttaatg ggttaccgct attcgacaca aggtttttat acccttgatg
     3001 atgttgcgta tcgtcgaatg gaggggtacg aatatgatta cgattacgac ggtgagcatc
     3061 gggatgaacc gataatcgtg aattaccaca atttacgctt tagccgtaaa gaccgtttgc
     3121 agttaaatat ttcacaatca cttaatgact ttggctcgct ttatatctct ggtacccatc
     3181 aaaaatactg gaatacttcg gattcagata cctggtatca ggtggggtat accagcagct
     3241 gggttggcat cagttattca ctctcatttt cgtggaatga atctgtaggg atccccgata
     3301 acgaacgtat tgtcggactt aatgtttcag tgcctttcaa tgttctgacc aaacgtcgct
     3361 acacccggga aaatgcgctc gaccgcgctt atgcctcctt taacgccaac cgtaacagca
     3421 acgggcaaaa tagctggctg gcaggtgtag gtgggacctt actggaaggc cacaacctga
     3481 gttatcacgt aagccagggt gatacctcga ataatgggta tacgggcagc gccacggcaa
     3541 actggcaggc cgcttacgct acgctggggg tcgggtataa ctacgaccgc gatcaacatg
     3601 acgttaactg gcagctgtct ggcggtgtgg tcgggcatga aaatggcata acgctgagcc
     3661 agcctttagg ggataccaat gttttgatta aagcgcctgg cgcaggcggt gtacgcattg
     3721 aaaatcaaac tggcatttta accgactggc gcggctatgc ggtgatgccg tatgccacgg
     3781 tttatcggta taaccgtatc gcgcttgata ccaatacgat ggggaattcc atcgatgttg
     3841 aaaaaaatat tagcagcgtt gtgccgacgc aaggcgcgtt ggttcgtgcc aattttgata
     3901 cccgcatagg cgtgcgggcg ctcattaccg ttacccaggg cggaaaaccg gtgccgtttg
     3961 gatcactggt acgggaaaac agtatcggaa taaccagtat gtgggtgatg acgggcaagt
     4021 ttatttaagt ggtgcgccat tgtctggtga attactggtt cagtggggag acggcgcgaa
     4081 ctcacgctgc attgcgcact atgtattgcc gaagcaaagc ttacagcaag ccgtcactgt
     4141 tatttcggca gtttgcacac atcctggctc ataaaggaaa ttatcaatga agataatctg
     4201 cagattatta ttggcgatgg catgtttgtg tctggcaaac atatcctggg ctactgtttg
     4261 tgcaaatagt actggcgtag cagaagatga acactatgat ctctcaaata tctttaatag
     4321 caccaataac cagccagggc agattgttgt tttaccggaa aaatccggct gggtaggtgt
     4381 ctcagcaatt tgtccacccg gtacgctggt gaattataca taccgtagtt atgtcaccaa
     4441 ctttattgtt caggaaacta tcgataatta taaatatatg caattaaatg attatctatt
     4501 aggtgcgatg agtctggttg atagtgtgat ggatattcag ttccccccgc aaaattatat
     4561 tctgatggga acagatccta acgtttcgca aaaccttcca ttcggggtga tggattctcg
     4621 tttaatattt cgtttaaagg ttattcgtcc ctttattaac atggtggaga tccccagaca
     4681 ggtgatgttt accgtgtatg tgacatcaac gccttacgat ccgttggtta cacctgttta
     4741 taccattagt tttggtggcc gggttgaagt accgcaaaac tgcgaattaa atgccgggca
     4801 gattgttgaa tttgattttg gtgatatcgg cgcatcgtta tttagtgcgg cagggccggg
     4861 taatcgacct gctggtgtca tgccgcaaac caagagcatt gcggtcaaat gtacgaatgt
     4921 tgctgcgcag gcttatttaa caatgcgtct ggaagccagt gccgtttctg gtcaggcgat
     4981 ggtgtcggac aatcaggatt taggttttat tgtcgccgat cagaacgata cgccgatcac
     5041 gcctaacgat ctcaatagcg ttattccttt ccgtctggat gcagctgcgg cagccaatgt
     5101 cacacttcgc gcctggccta tcagtattac cggtcaaaaa ccgaccgaag ggccgtttag
     5161 cgcgctgggg tatttacgcg tcgattatca atgaggtacg gagaatgaga agagcactct
     5221 ttagctgttt ctgcgggtta ctgtggattt ccagtggatg ggcagctgac cctttgggaa
     5281 cgattaatat caatttgcac ggtaacgttg ttgatttctc ctgtaccgta aacactgcgg
     5341 atattgataa gacggtagat ttaggcagat ggcctacgac acaattactg aacgctggcg
     5401 ataccacggc actcgtccct tttagcctgc ggctggaggg atgtcctccg ggttcagttg
     5461 cgattttatt tacgggaacg ccggcatccg ataccaacct gctggctctg gatgatcccg
     5521 caatggcgca aaccatcgcc atcgaattac gtaatagcga tcgctcccgg ctcgcactgg
     5581 gagcggcgag cccgactgag gaagtagatg caaatggcaa tgtcacacta aacttttttg
     5641 ccaattatcg agcgttagcc agcggtgttc ggccaggtgt ggcgaaagcg gatgcgatat
//
LOCUS       DAZFWP020000235         5649 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFWP020000235 DAZFWP020000000
VERSION     DAZFWP020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.237"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(110..964)
                     /gene="rclR"
                     /locus_tag="ACHK0Z_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             complement(110..964)
                     /gene="rclR"
                     /locus_tag="ACHK0Z_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU4504588.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1190..2515
                     /gene="rclA"
                     /locus_tag="ACHK0Z_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             1190..2515
                     /gene="rclA"
                     /locus_tag="ACHK0Z_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGU4504589.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILHDQIFTHPSMSESLNDLFSLVK"
     gene            2624..2860
                     /gene="rclB"
                     /locus_tag="ACHK0Z_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     CDS             2624..2860
                     /gene="rclB"
                     /locus_tag="ACHK0Z_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU4504590.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            2872..3465
                     /gene="rclC"
                     /locus_tag="ACHK0Z_RS23585"
                     /old_locus_tag="pgaptmp_004717"
     CDS             2872..3465
                     /gene="rclC"
                     /locus_tag="ACHK0Z_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU4504591.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(3625..4494)
                     /locus_tag="ACHK0Z_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             complement(3625..4494)
                     /locus_tag="ACHK0Z_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4504592.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLAKFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            4743..5600
                     /locus_tag="ACHK0Z_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             4743..5600
                     /locus_tag="ACHK0Z_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4504593.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
BASE COUNT         1613 a         1249 c         1223 g         1564 t
ORIGIN      
        1 tcagaaaaaa atatataaag cccaaaccac cgttggaaat agaaatagtt gaaaggctca
       61 caactcccgg aatgcggatt atctttgccc tgatttctgg cggttttatt cagggtgcaa
      121 gctgtctgac cctttcccga tattctcccg gagtacaacc aaactcgcgg acaaacgcct
      181 tgtgaaaaga tgattcactg gcatagccta ctgactcagc gatcaccaca acagggagca
      241 tttcccggga aaacatctgg gccgctattt gtagacgcaa ctttgttaat acagccagcg
      301 gcgtggttcc ggaaacatca cggaaaagct gggcaaaact tgcccgggac atgtgagcga
      361 tgctggccag cgattcgacg gtccaggcgt gtcctggcat ttccagcatt tgctgtatta
      421 ccgcaccaag acgtggatgc agaagcaaac tgagaatgtt tttctcagta ttaacctgtg
      481 caatccactc acgcaccgcg agggtaaaga atgtagcgca gatctggcta cacagtgcat
      541 ccactcccgg catcgccgat ctggattcct gttgtaaaaa cgggatcgcc tccctcagcc
      601 agttatattc cacgctgtgg ttaaccggtg ctaaaaacag cgtttccggc aaagacgtta
      661 aaaaataacg cgccgaatgt tgcaaccgaa gagtgccgca gacaatacag gtcgattcat
      721 tatcgacatg actcaggcga tgagctgaat tttgtggtag caggaccaca tttcccgggc
      781 gtaatgtaaa aatctctccc gtcggcattt ccagcttcgc cgctccttgc gttaacgcat
      841 gccaacgaat aaccgataat tccccggcac catgtggaag ctgccagtca cttcctaaca
      901 cgcaattctt atcgatcgtt ccttgtggag cgttaagcat caacaaacgg ctaagggcat
      961 ccatatcaga ctcctgagtg gtttttgatt acaaacacca acaatcttag taaataacta
     1021 atctaatacc atcaaatggt taatcatgat ttttgttttg tgattctgat taagaaaaag
     1081 aaaataccag acgataagac aaaatatcaa gcgttgtgaa gaaatggtat ttgctctttt
     1141 gcgtctatag tcatgatgtc aaatgaacgc gtttcgacag gaaatcatca tgaataaata
     1201 tcaggcagtt attattggtt ttggcaaggc tggaaaaaca ttagccgtca cgctggcaaa
     1261 agcaggttgg cgagtggctc tcatcgaaca atcaaatgca atgtatggcg ggacctgtat
     1321 taatatcggc tgcatcccaa ccaaaacatt ggttcatgac gcacagcagc acacagattt
     1381 tgtccgtgcc atacagcgta aaaatgaagt ggttaatttt ttacgtaata agaattttca
     1441 taatcttgcg gatatgccca atatcgacgt gatcgacggc caggcggagt ttatcaataa
     1501 tcatagcctg cgtgttcatc ggcctgaggg aaatctggaa attcatggcg agaaaatttt
     1561 tattaatacc ggtgcacaaa ccgtggttcc gccaattcct ggaattacca ccacgcctgg
     1621 tgtatatgac agcaccggat tacttaatct aaaagaattg cctgggcatt taggtatttt
     1681 gggcggcgga tatattggcg ttgagttcgc ctctatgttc gctaattttg gcagcaaggt
     1741 aaccatttta gaagcagctt cgctgttttt gcctcgggaa gatcgggata ttgctgataa
     1801 tatcgcgacg attttacgcg atcagggcgt cgatattatc ctcaatgccc atgtggagcg
     1861 aatcagtcac aatgaaaatc aagtgcaagt gcatagcgag cacgcccaac tggcggtgga
     1921 tgcactgtta atagcttccg gtcgtcaacc ggctaccgct tcgttacatc cagaaaatgc
     1981 cggtatcgca gtaaacgagc gcggggcaat tgtcgttgac aagcaattac ataccaccgc
     2041 agacaatatt tgggcgatgg gagatgttac cggcgggctg caatttactt atatttcact
     2101 ggatgattac cgcattgtgc gtgatgagtt gctgggagaa ggcagacgta gtactgatga
     2161 tcggaaaaat gtgccttatt ccgtattcat gacaccgcca ctgtccaggg ttggtatgac
     2221 agaagaacag gccagagaga gtggtgctga tattcaggtg gtgacattgc ctgtagctgc
     2281 aattccgcgt gccagagtga tgaatgatac tcgtggggta ttaaaagcga ttgttgataa
     2341 taaaacccaa cgtatattag gggcatcact gctgtgtgtt gactcccacg agatgattaa
     2401 tatagtgaaa atggtgatgg atgccgggct gccttatagc atattacacg atcagatatt
     2461 tactcatccg tcgatgagcg aatcactcaa tgatctattt tcattagtca aataaactca
     2521 aaatcagacg ccagaacaaa tattctggcg tctcagagaa aagaatctta ttaattccct
     2581 gttactctat aacttcttaa tcacttcatt gatggtattt tatatgttca aaaaatctgt
     2641 tttatttgca acactattat ctggcgttat ggcattttcc accaatgcag atgataaaat
     2701 aattctgaaa catatcagcg tctcgtcagt atcagcatca ccgacagttc tggaggatac
     2761 cattgctgat atagccagaa aatataatgc ttcatcctgg aaagtcacat cgatgcgaat
     2821 tgataataat tcaaccgcaa cagcagtatt gtataaataa ggatgttcac aatggaaaaa
     2881 tacctgcacc tgttaagtcg gggagataaa attggcctga cattgattcg tctgagtatt
     2941 gcaattgttt ttatgtggat tgggttatta aagtttgtcc cttacgaggc agacagcatt
     3001 acaccattcg tcgcaaacag tccactaatg tcgttctttt atgaacaccc ggaagactat
     3061 aaacagtatc tgactcacga aggcgaatac aaaccagaag caagggcatg gcaaacggcc
     3121 aataatacct atggtttttc caacggtctt ggcgtcgtgg aggtgattat tgctctgctg
     3181 gttttggcta atcctgtcaa tcgctggtta ggtttattgg gaggactgat ggcatttacc
     3241 acgccgctgg taacactctc atttttaatc accaccccgg aggcatgggt acccgcattg
     3301 ggtgacgctc atcatggttt cccttattta tccggtgctg gtcgcctggt attgaaagat
     3361 actctgatgc tggcaggtgc tgtcatgata atggcagatt cggcgcggga aattcttaaa
     3421 caacgcagta atgaatccag ttcaacgtta aaaactgaat attgataatc cgaacactgt
     3481 ctgttgttca ctttttcagg cgtcaggccc cctttctgat gatgaagcct gacgccatat
     3541 taccggagat atgacagagt gaagatcaat atcactcccc tgttaaaaga ttcttgtcgt
     3601 ctcaggtaaa atatatgatt aatatcaatc atgtattttt acagcaagac aaccacgaac
     3661 aaattcagga ttaaaatgtt ttaccgactc cccaacataa cccaaatcaa gcgaactaat
     3721 ttgtgccatt tctttctctg tcagtgagaa atcccagata gcaaaatttt cttcgatacg
     3781 ctgttgatgt gtcgatttcg gaataacgac cactccccgt tgaatattcc aacgcagaat
     3841 gacttgagaa atcgattttt gatgggcatc tgcaatactc tgaagcagat tattttcaaa
     3901 gggtttatgt cgtccaccac ctaacggagc ccatgcttca ggctgcacgt tataatactt
     3961 catggttgcc agcgcctctg gttgggcaaa atagggatgc aactcgacct ggttgaccat
     4021 cggtttaact cttaccgttt cacaaaattt cgccagaaca tgaggataga aattggaaac
     4081 cccaattgct tttaatttgc cctcttcata ggcatcttcg agtgcacgcc acgcactgaa
     4141 ataatcgcgc atagcctggt gcaataaata gaggtcgaag tactctagtc ccgatttttt
     4201 taatgatgct tcaatacctg ctgctgccgt atcctgattc aacatatcct gcacccacaa
     4261 tttagacgtt ataaataact catctcgggt acataaacct tcagatatag cttcacgaac
     4321 ggcttcaccg acggcatctt cattaccgta taccgctgca gtgtcgatga gtcgataacc
     4381 tgtgcggata gcgttcaaca ctgactgttt gcacacgttt ttatcggtaa cctgaaaaac
     4441 accaaatccc atcatcggca ttttcagatt gttacttaat acggaaaatt ccactgtcag
     4501 tccctccatt aggcatgaac aatgagtcta cgttaaaacg taacctcaaa ttagtacgtg
     4561 gattttgata tcacttatgc aaaaaattca ttaataatgc aggactgaaa cctctctatt
     4621 ttcggggaca acgaagcaga cgctaccagt gcttttgcct tcgcccttgc tgtttttgat
     4681 acacttaggg cccagggcat aacgaaaatg tgcgatatga cgaagaatca atggagaatg
     4741 tgatgatcag gcagaagatt ctacagcagc tcctggagtg gattgagtgc aatcttgagc
     4801 accctatttc aatcgaagat atcgcacaga aatctggcta cagcagacgc aacatccagc
     4861 ttctgttccg aaatttcatg catgtgcctt tgggagaata cattcgcaaa cgaaggcttt
     4921 gtcgtgccgc cattcttgtc cggctcaccg cgaaatctat gcttgatatt gcactctctt
     4981 tgcattttga ttcacagcag tcattcagcc gcgaatttaa aaagttattc ggctgctctc
     5041 cccgtgtata ccgccaccgt gattattggg atctcgcaaa tatcttccct tcttttttaa
     5101 tacgtcaaca gcaaaagacc gagtgtagat tagtcaactt tcctgagaca cctatttttg
     5161 gcaattcatt taaatatgat attgaagtgt cgaataaatc accggatgaa gaagtcaaac
     5221 tgcgacgtca tcatttagcc agatgtatga ataattttaa gacggatatc tatttcgtct
     5281 ccacgtttga accgtcaaca aaatcggtcg atttgctcac ggttgaaact tttgccggta
     5341 cggtatgcaa acaaaccgat tcagagatgc cgaaagagtg gacaattaac cgaggactgt
     5401 atgcctcttt ccgctatgaa ggtgagtggg agcactaccc tgaatgggcg cgaaacctct
     5461 atctgatgga attacccgcc agggggttag ccagagtgaa cggcagtgat attgagcggt
     5521 tttattacaa tgaaaatttc gtcgaaattg atagcaataa cattgtttgc gaaattttta
     5581 tccccgttcg tccggtttag ttttcgctat atctaaatga atttataacg agactactgt
     5641 caggaaatt
//
LOCUS       DAZFWP020000236         5636 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFWP020000236 DAZFWP020000000
VERSION     DAZFWP020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.238"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            212..287
                     /locus_tag="ACHK0Z_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     tRNA            212..287
                     /locus_tag="ACHK0Z_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Thr,seq:ggt)
     gene            327..442
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     rRNA            327..442
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(674..1432)
                     /gene="yhdZ"
                     /locus_tag="ACHK0Z_RS23615"
                     /old_locus_tag="pgaptmp_004723"
     CDS             complement(674..1432)
                     /gene="yhdZ"
                     /locus_tag="ACHK0Z_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU4504594.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1440..2543)
                     /gene="yhdY"
                     /locus_tag="ACHK0Z_RS23620"
                     /old_locus_tag="pgaptmp_004724"
     CDS             complement(1440..2543)
                     /gene="yhdY"
                     /locus_tag="ACHK0Z_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4504595.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2553..3734)
                     /gene="yhdX"
                     /locus_tag="ACHK0Z_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             complement(2553..3734)
                     /gene="yhdX"
                     /locus_tag="ACHK0Z_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4504596.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3802..4827)
                     /locus_tag="ACHK0Z_RS23630"
                     /old_locus_tag="pgaptmp_004726"
     CDS             complement(3802..4827)
                     /locus_tag="ACHK0Z_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4504597.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5258..5479)
                     /locus_tag="ACHK0Z_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             complement(5258..5479)
                     /locus_tag="ACHK0Z_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504598.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1555 a         1436 c         1359 g         1286 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cttagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaatttagcg tgctgatatg gctcagttgg tagagcgcac
      241 ccttggtaag ggtgaggtcc ccagttcgac tctgggtatc agcaccactt tttaggttaa
      301 agttcggcag attagaaaag aatttgtctg gcggcagtag cgcggtggtc ccacctgacc
      361 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      421 cgagagtagg gaactgccag acatcaaata aaacataagg ctcagtcgga agactgggcc
      481 ttttgtttta tttgatgttt gtcggtgaac actctcccaa ccaggacaaa tccgccggga
      541 acggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
      601 actgccagac atcaaatcaa gcaaaaaggc catccgaaag gatggccttt ttgcttttcg
      661 aactaacatt caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag
      721 gatgcgtaaa aaattcatca ggtgcagctt gctccactat ttccccacga tccataaaaa
      781 tcacccggtc agcgacggtt cgcgcaaacc ccatctcatg cgttacacac aacattgtca
      841 tacccgattg cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga
      901 gcgccgacgt tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa
      961 tggcaacgcg ttgctgctga ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg
     1021 caattctcac ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct
     1081 tgcgtaccca aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat
     1141 tgaaatgctg aaagaccatt cccacttcct gccggacgcg ctcaatattg cggatatctt
     1201 cattaagttc gatgccatct accacgatcc gtccctgttg atgttcttcc agatgattaa
     1261 tacaacgaat ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc
     1321 ccggttgcac ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga
     1381 cgttttccag cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct
     1441 caatgcggtg tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg
     1501 ctgaaacaga agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc
     1561 aaccaggtgg gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata
     1621 atgatcacca ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt
     1681 accagcttca acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc
     1741 agcgactctg ccgcttcata ttgcccttta ggcagcgcct gtaatccgcc tcgcacgact
     1801 tccgcaacat atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg
     1861 atattggtgc cttctgccat aaacaaaggc agcatgaccg aagacataaa caatacggta
     1921 atcagcggta cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc
     1981 atatgggagc ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca
     2041 acggatgcaa taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctcc
     2101 agaccaaaaa aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag
     2161 accgcaatat agcgaccgcg atgcgggagt attttccaga acattggtgc gattgacaca
     2221 aggccaatca gtaatgccag gttaattcgc cagcgttggt cgtgtgggta aagtccatac
     2281 ataaactgac caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg
     2341 gcacgcgttg agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc
     2401 agttcccaca tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac
     2461 agattttttc gcacccacac catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc
     2521 tgagacagca atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg
     2581 ttatagatat tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc
     2641 atggctatcg tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa
     2701 accatatcgg gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac
     2761 tggctggtta acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc
     2821 gtaaccgggt tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc
     2881 tggatcccag cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc
     2941 gttaaggctg ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg
     3001 gctgggacat cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca
     3061 atgatcaaca ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat
     3121 gttttattaa agcggaatag cccaacagaa aggactatag ccataacaac agccaggata
     3181 aacgcaataa atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa
     3241 aaggccagat caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca
     3301 aagtaccaga agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt
     3361 gtggatagtt ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagcct
     3421 aaaacagaag cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca
     3481 ataaaaacgc gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg
     3541 aagccagcgc cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc
     3601 gttacagtgt tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc
     3661 tggaataacc aggcgcgaac cgtagggttg gcaaaagaga gtgagccttt aacggttgag
     3721 cggcgatgag acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga
     3781 cgcccgttac ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt
     3841 ccagagatta ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc
     3901 gaaaatttcc gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccag
     3961 cttcagatcc ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc
     4021 tggattagcc gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag
     4081 catggcgaaa agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac
     4141 cggcccaagc ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct
     4201 taatttgatg cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc
     4261 agattccagt gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat
     4321 attgtttgcc ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca
     4381 gacggtagcg ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa
     4441 gccaatgccg tcgtaataag tgacgccagt aaatgccatt cccatcccgg cgtcgcgaga
     4501 tgaagtccag gtcgtattac gggagagcaa atccacttcc cctgactgta aagccgtgaa
     4561 gcgttctttt gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc
     4621 ggcaacacca cgacaaacat caacatcaat acctgaaaac ttaccgtctg catcggcata
     4681 agagaaccca ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc
     4741 atcaagcgtc gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc
     4801 cagtgtggct atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata
     4861 ggacgtttgg gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg
     4921 ccagcttttg cgcccgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa
     4981 acagcgcccc gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta
     5041 cgcgtgtttt actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggaagtg
     5101 tgaaagttaa attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa
     5161 atcagcagag aattagccag cagaatatag caatatttta tcttatgtaa agatactgta
     5221 tcgttaaaca gagcgcgaaa ctccgcgccc tgttcagtta tttcgacaga gcgatgatcc
     5281 ccatcgtcag gccagcaaca gccgctgccc cgccggcaat agccccagcc gtttcccagt
     5341 tatcctgagt cgtgcctttc atacaggtat tggtgtgaac cagacgaccc tgatcgtcat
     5401 aaaccggaac gcagggcgaa tcgtgagcac agcccgccag caatgcggtg agcaatgcca
     5461 caggaattaa tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa
     5521 ctctcttatt aacgagctga atgccatttt atcaccggag gttataaata ttgcggaatg
     5581 aataatgaca aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaa
//
LOCUS       DAZFWP020000237         5559 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFWP020000237 DAZFWP020000000
VERSION     DAZFWP020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.239"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..179)
                     /locus_tag="ACHK0Z_RS23640"
                     /old_locus_tag="pgaptmp_004728"
     CDS             complement(<1..179)
                     /locus_tag="ACHK0Z_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504599.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALQERPNID
                     VQRVTLDDGGVDQRAL"
     gene            complement(163..441)
                     /locus_tag="ACHK0Z_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     CDS             complement(163..441)
                     /locus_tag="ACHK0Z_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4504600.1"
                     /translation="MHKCLKNKIRQIGLSQAGVARLIGLPQQVVCRWVNGQQVPASRV
                     LQLCEIMAWTVTPHELRPDIYPNPTDGLPCGRKSTANELLAVNNENHT"
     gene            558..956
                     /locus_tag="ACHK0Z_RS23650"
                     /old_locus_tag="pgaptmp_004730"
     CDS             558..956
                     /locus_tag="ACHK0Z_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4504601.1"
                     /translation="MTTLEITTYRIKLVLEKTGLKQAELARRIGVAQQSVQKWVHGIT
                     SPSMANLDKLSEVTGFPPYWFMLPPSEDEQIVVPDTMKIGPKQRELLQTFGAFPEEEQ
                     GQMLQEMKDKKELMERTIERWLAARKGHRA"
     gene            986..1174
                     /locus_tag="ACHK0Z_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             986..1174
                     /locus_tag="ACHK0Z_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGU4504602.1"
                     /translation="MNTALSPMVSEFETIEQENSYNEWLRAKVAASLANPRPAIPHDE
                     VERRMAERFAKMRKERSK"
     gene            1178..1465
                     /locus_tag="ACHK0Z_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             1178..1465
                     /locus_tag="ACHK0Z_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4504603.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVATLSIEIVSVVHARRQFPKQN"
     gene            1737..1889
                     /locus_tag="ACHK0Z_RS23665"
                     /old_locus_tag="pgaptmp_004733"
     CDS             1737..1889
                     /locus_tag="ACHK0Z_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4504604.1"
                     /translation="MDTLNLGNNESLVCGVFSNLDGTFTAMTYTRSKTFKTEAGARRW
                     LARNTD"
     gene            1901..2269
                     /locus_tag="ACHK0Z_RS23670"
                     /old_locus_tag="pgaptmp_004734"
     CDS             1901..2269
                     /locus_tag="ACHK0Z_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504605.1"
                     /translation="MEFKDLPVPFQEMAANIVRSQLATLDLSTVEKETIDNISHNVRN
                     AFIKLYEEKLSSENKDYSPKFTPHPGGVSEIYHETMVKALRMQIAELEHKLNLVSKCQ
                     NQHKAKGLHFHHKGKQKCEH"
     gene            3054..3242
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     CDS             3054..3242
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4504606.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            3239..3427
                     /locus_tag="ACHK0Z_RS23680"
                     /old_locus_tag="pgaptmp_004736"
     CDS             3239..3427
                     /locus_tag="ACHK0Z_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4504607.1"
                     /translation="MSSAFTLMMTVFLITGEPQKVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            3520..>5559
                     /locus_tag="ACHK0Z_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     CDS             3520..>5559
                     /locus_tag="ACHK0Z_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HGU4504608.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSNLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHATPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEAKKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDDDDQNQDKAEQ
                     NSPNALQNEPKTKQPEPVVQQEPEKVCTTCGQTDGGNCPDCGAVMGDATYQETFNEES
                     QDEARKKNPEEMENAVLPNKQCTEGDQHANGNNETGETANPLITVNGHHVITSTSRTC
                     DHLMIDLKTMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIK"
BASE COUNT         1674 a         1289 c         1286 g         1310 t
ORIGIN      
        1 agcgcacgct gatcaacccc gccatcatca agcgtgacac gctgaacatc gatgttcggg
       61 cgttcctgta gagcccagaa ggcttcagta attaatatcg tcgcctgctc ctgcgtcatt
      121 cctggtctgc atacccaggc atccagagcc tcgcaaacct gttcaggtgt gattttcatt
      181 gttcaccgcc agtaattcat tcgctgtact cttacgtcca caaggcaggc catcggttgg
      241 gttagggtaa atatctggtc ggagctcgtg gggggtgaca gtccacgcca taatctcaca
      301 tagttgtaaa acccttgatg cagggacctg ttgcccatta acccatcgac atacaacctg
      361 ctgtggcaac ccaatcaagc gagcaacacc cgcttgggat aaccctattt ggcgaatttt
      421 attttttaaa catttatgca tatcgctaac tccaatcaat cgacaccact tatacactct
      481 aaaagttaca ctatcaacac tttcaagctg attgtgctgg ctacactttt tttgtgtaaa
      541 atgaatgcat gaagcgaatg accactcttg aaataaccac gtaccggata aaactggtgc
      601 tggaaaaaac agggcttaag caagcagaac tagcccgcag aataggtgtt gcccaacaat
      661 cggtccaaaa gtgggttcat ggcatcacaa gcccatcaat ggctaatctt gataaacttt
      721 ctgaagtaac aggattccct ccatattggt ttatgttgcc accaagtgag gacgaacaga
      781 ttgtggtacc tgacacaatg aaaataggtc cgaagcaaag agagctactt cagacatttg
      841 gtgcattccc agaggaagaa cagggacaaa tgcttcagga gatgaaagac aaaaaagagt
      901 taatggagcg cacaattgaa agatggctcg ctgcccggaa agggcatcga gcataatgac
      961 caccatacac agacggaggt gtgccatgaa tacggccctt tcaccaatgg tttctgagtt
     1021 cgaaaccatt gaacaagaaa acagttacaa cgaatggctg cgtgcgaaag tagcagcgag
     1081 ccttgcaaat ccgcgcccag caattcccca tgacgaggtt gagcgcagaa tggcagaacg
     1141 ctttgctaaa atgcgcaagg aacggagtaa gtagtaaatg ttacccgtgt tatggcttga
     1201 aagcgcagat accgacctag atgatataac tagttatatt gctcgtttcg acatagatgc
     1261 ggcagaacgc ttatggcagc gattaagggg gtgtgtgctg ccgttatccg aacacccgta
     1321 tttataccca ccaagcgaca gagtacctgg cttgcgtgag attgtagctc accctaacta
     1381 tataattctt taccgtgtag ccactttaag cattgagatc gtgagtgtag tacacgccag
     1441 acgccaattc cccaagcaga actaaccctt tcacagggcc gtctcatctc gaggcggttt
     1501 ttgttgccca caacctaact ttataagtgt tgacataata actttaaaag tgcatccttg
     1561 tttcaccaac ccaccccatt caacagaacg cagggcaata cttcgagtta cccggcagtg
     1621 gtcaggggtt aagtagccag cccgaggcgt atgaacatga cggcgggaac actttgtata
     1681 acagcgcagc aggtttttag ttccgcgacc cggcgttaag ggtaaatgag gtcaacatgg
     1741 atacgctcaa tcttggcaac aacgaatctc tggtatgcgg agtattctcc aacctggacg
     1801 gcacgtttac cgcgatgacg tataccagaa gcaaaacgtt taaaactgaa gctggcgcgc
     1861 gtcgctggtt agccagaaac actgactgat gaggttgacg atggaattta aagatttacc
     1921 agtaccattc caggaaatgg cagcgaatat agttcgttct caactggcga ctcttgacct
     1981 gagtaccgta gaaaaagaaa ccatcgacaa tatatcccat aatgtgcgta atgcctttat
     2041 caagctgtac gaagagaagc tgagttctga aaacaaagat tattcaccta aattcacccc
     2101 acacccaggt ggagtgagtg agatttatca tgaaacaatg gttaaagcac tacgtatgca
     2161 aatagccgaa cttgaacata aattaaattt agttagtaag tgccaaaatc agcacaaggc
     2221 aaaagggttg cactttcatc ataagggcaa acaaaaatgt gagcattaac accaactgaa
     2281 ttagcaacag aaaaggtttg ttcaagtatt tgattcgaat tgagcgttga ctgaataacg
     2341 tatgcccctg gcaacaattt atattcaacg tcaccttcat taatcatcct ggagtattta
     2401 aaatttggca tcagagtctc gaactttttc tcctgttcca ttgttgcttc aaataactgc
     2461 acatgaacat aatatcgatt tgatttagag tgcattttat cctccataga ggttctgggt
     2521 taaaaatgga gaccaacacg ctgtcacgtg tggtcgtgcg ccggacacgg ataagaatcc
     2581 ggcactgaca gtttactgaa agaacatatc cctgaaaagt cagggcataa cacgaaagcg
     2641 cacggcgaag ttagtctctc tgtacaggtc gtcgttaaat ttaattcgat cgtgcgcttc
     2701 cggttgtggc aatccgcgaa atggcgcggc ggtaagtatg gctggggctt cctccattac
     2761 tccagaaaat gcaccgggtt gtcaggttga ccatacgcct gagtgacaac accgccacaa
     2821 caacctctgt tatcactttt ctggtgattc ggcggaaatg gatatccgcc atttttaaag
     2881 tgtattttgt gatgcggtga atgcggctaa gcgcacgcgg aacagttaaa acaagcggtc
     2941 ttttactggc gtaacagaca tcaactaaca atccggcgtt aattgttaac tggttaacgt
     3001 cacctggagg caccaggcac cacatcacaa aattcattgt tgaggacgcg ataatggaaa
     3061 cgtcactacc aaacgttaat acgtctgaag ggtgttttaa tattggtatt ctgctcagta
     3121 accgggagtt tactgaggac gccatcagga tgagaaaata tgagccttat cttctcaatg
     3181 ataattccat actctccaga attgcccttc ttgaacttgg cattttcgga gggcagcagt
     3241 gagttcagcg tttacactga tgatgacggt ttttctgata acaggtgagc cacagaaagt
     3301 gattaccgga atttatgcca gtaaagaatc atgcctccgt gcaagagacg agcaaaaaat
     3361 ttctggcgaa tgtctcccgt taaaaaaagt atcgctgtac ctgaataacg aaataccggc
     3421 tggataaccc gccagccata ttaacgccat acccgttgat taagtatgcc agcaatggca
     3481 gggattcgta caaccttaaa atagttatga ggtttatcaa tgagcactga taaagaagaa
     3541 attgcactgt attacgaagc caaaaatgac aaagtcagaa aacgccttgg gattaaaggc
     3601 ggtttttact ggcgcacagc aaaaaaatta tcggttgcaa tatcacgggg tgttgtcgca
     3661 atggacgatg ctggatttga cgaagaggat ttcaaaaaac ctgttcgcgt gaatttgcct
     3721 attgttaatg acctgccgcc tgaaggtgtg tttgataccg aattctgcaa ccgctatgaa
     3781 aaaggcggag aagatggcat cacaatgata tttatagcgc cttccccctc agttcaggac
     3841 aaacctgcca gcactgacaa taccaacgtc aatggcgaag acatggctga gattgaggat
     3901 aatatgctcc tgccgatttc cggtcaggaa ctgcccattc gctggcttgc gcaacatggc
     3961 agcgaaaaac cggtaacgca cgtttcacgg gaagaacttc aggcattaca tatcgcacga
     4021 gctgaagaac tgcctgctgt tactgccctg gctatttccc acaacacaaa gctgctcgac
     4081 ccgctggaga ttcgcgacct tcacaaactg gtacgcgaca cagacaaagt tttccctaat
     4141 cccgttaatt ccaatctggg gttaatgact gcttttttcg aagcatacct ggacgctgac
     4201 tataccgatc gaggtctgct gacaaaagag tggatgaaag gaaatcgtgt tttacgcatc
     4261 agccgcacgc catccggcgc taatgctggc ggaggaattc ttaccgatcg cggtgaaggt
     4321 tttgtccacg atgatgcgtc agtggaacgt gacgttgccg ctggcgttct ggcccgttca
     4381 atggacatcg atatttacaa tccacatccg gcacacgcca aacgcattga agaaatcgtt
     4441 tcagagaata agccgccctt ttctgttttt cgtgacaaat tcatcgccat gcctggtcac
     4501 ctggattatt cccgcgcgat agtggttgcg tccgtgaaag aagcaccaat tggtatagag
     4561 gctactcccc accgtgtcac cgaatacctg aataaggtgc tgactgaaac cgatcatgcc
     4621 acccctgatc cggaaatcgt ggatattgcc tgtggtcgct cctctgcccc gatgccgcag
     4681 cgtgtaacaa aagaaggaaa acaggatgat gaagaaaaac cgcagccatc tggcgcaatg
     4741 gcagatgaac aggcaacgac tgaagcagtg gaaccggata caactgaaca taatcaggac
     4801 acgcagtcga tggatgctca gccacagata aattctgttg atgcgaaata tcagaaactg
     4861 cgtgcagaac tccatgaagc caagaaaaac attccaccca aaaatcctgt cgatgcagat
     4921 aaattactgg ctgcttctcg aggagaattt gttgaaggga ttagcgaccc gaatgatcca
     4981 aaatgggtga aggggatcca gagtcgcgat gatgatgacc agaatcagga taaagcggaa
     5041 caaaacagcc caaatgcgtt acaaaacgag ccaaaaacaa aacaacctga accagtagtg
     5101 caacaagaac cagaaaaagt ttgcaccaca tgtggtcaga ccgatggcgg caactgccct
     5161 gattgtggag cggtaatggg tgacgcaacc taccaagaga cattcaacga agaaagccag
     5221 gatgaagccc ggaaaaaaaa tccggaggaa atggaaaatg ccgtgctccc gaataagcaa
     5281 tgcaccgaag gcgatcaaca tgccaatggc aataatgaaa caggcgagac agcaaatccc
     5341 ttaattacgg tgaacggtca tcacgttatc acatccacca gcaggacgtg tgaccatcta
     5401 atgatcgacc ttaaaaccat gggaaaaaat cctgatgccc cgattatctc aataggtgca
     5461 atatttttcg atccgcaaac cggagatatg ggaccggaat ttagtaagac tatcgatctg
     5521 gaaactgctg gcggagtcat tgatcgggac accattaaa
//
LOCUS       DAZFWP020000238         5533 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFWP020000238 DAZFWP020000000
VERSION     DAZFWP020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.240"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..302)
                     /locus_tag="ACHK0Z_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             complement(<1..302)
                     /locus_tag="ACHK0Z_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4504609.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLL"
     gene            501..1199
                     /locus_tag="ACHK0Z_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             501..1199
                     /locus_tag="ACHK0Z_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4504610.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1244..2143)
                     /gene="eamA"
                     /locus_tag="ACHK0Z_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             complement(1244..2143)
                     /gene="eamA"
                     /locus_tag="ACHK0Z_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU4504611.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGACTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2338..3525
                     /gene="ydeE"
                     /locus_tag="ACHK0Z_RS23705"
                     /old_locus_tag="pgaptmp_004741"
     CDS             2338..3525
                     /gene="ydeE"
                     /locus_tag="ACHK0Z_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU4504612.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3652..3747
                     /gene="mgtS"
                     /locus_tag="ACHK0Z_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             3652..3747
                     /gene="mgtS"
                     /locus_tag="ACHK0Z_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU4504613.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3747..3851
                     /gene="mgtT"
                     /locus_tag="ACHK0Z_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             3747..3851
                     /gene="mgtT"
                     /locus_tag="ACHK0Z_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU4504614.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3966..4856)
                     /gene="dgcZ"
                     /locus_tag="ACHK0Z_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             complement(3966..4856)
                     /gene="dgcZ"
                     /locus_tag="ACHK0Z_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4504615.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTSALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5111..5503)
                     /gene="ydeI"
                     /locus_tag="ACHK0Z_RS23725"
                     /old_locus_tag="pgaptmp_004745"
     CDS             complement(5111..5503)
                     /gene="ydeI"
                     /locus_tag="ACHK0Z_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU4504616.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1512 a         1332 c         1164 g         1525 t
ORIGIN      
        1 agtagtttca tcgcaaattc aagaactgcc tgtccgttca tggtgccgta gtctttcatc
       61 tcgataacgg ctacaggcat tttcccctgt gcaacggctt caatctctgg tttctgaaag
      121 cgaacttgcg ggccaagtaa aacaacgtca acttcgttat ttttaatttt ccctttagcc
      181 tctgcaatcg ccaatgcgga aatattaact tcaagtgaaa tagcgttagc atgatcaatc
      241 atacgtttaa ccagcatact ggttgacatg cccgcagcgc aaactaacaa tatttttttc
      301 atattttccc ttactggtat ataacagatt acattgttta aatatcatat cccgcagtag
      361 ctatacgtcc tggtacattg atcacagtaa tacagtgata aagttgttca acatcagggt
      421 gattattaaa cgactcggcc atacgataaa ccaatcagaa ctcaactcct tgttttcaat
      481 ggctcttaaa aggtgcaaaa gtgatttttc aaaagattgc ccgcttactt aaatccgaaa
      541 tcaatggcaa ttcatggcat gttggtgatt tgttgccgtc agaagcggaa ctcgctgttc
      601 gttataatgt ttcgcgtaat accctgcgta aggcattatc cttgctggaa ggcgaaggaa
      661 ttattcacag aaagcatggt tcaggaacat acattcaaaa aaagaatttt gttgcacaca
      721 ttgatcacat gaacagtttc agtgaaattg cgcataaaag cggcaaagag gcagggagtc
      781 agattatgaa atttgaagtg caggatgctt cacccactat tgcgactgag ctgaatttag
      841 tgactggtga gcaggtttat tacataaaga gactgcgatt cattgaggat aatgcagcac
      901 aattagaaga aacgtggatg tcagtagcac gttttcctga tttaaccgtg tcacatatgc
      961 aaaaatccaa attttcatat attgagaatg aatgcgggat caaaatcatt ggcacctttg
     1021 aaactttctc ccctactttt cctaccccag aaatcgccag tattttacgg atcagcccac
     1081 gggatcccat acttaaaatt cagacccagg ctgtggacag taactctatt ccgctggatt
     1141 attcgttact ttacagcaat attttcgagt tccaggtaaa gtactttttc ccgcgataaa
     1201 agcagacgca cagagtgctg acatcacatt ctgtgcgtat ggtttagcct ctaaccttta
     1261 ccgctttacg ccaccgcaag ccaaatacat tgatatacag cccggtcata atgagcaccg
     1321 cacctaaaaa ttgcagaccc gttaagcgtt catccaacaa tagtgccgca cttgccagtc
     1381 cgactacggg caccagtaac gataacggtg caacccgcca ggtttcatag cgtcccagta
     1441 acgtccccca gatcccataa ccaacaattg tcgccacaaa cgccagatac atcagcgaca
     1501 agatggtggt catatcaata gtaaccagac tgtgaatcat ggatgcggaa ccatcgagaa
     1561 tcagcgaggc aacaaagaag ggaatgattg ggattaaagc gctccagatt accagcgaca
     1621 tcaccgccgg acgcgttgag tgcgacatga tctttttatt gaagatgttg ccacacgccc
     1681 aactaaatgc tgcagccagg gtcaacataa agccgagcat cgccacatgc tgaccgttca
     1741 gactatcttc gattaacacc agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt
     1801 tgccatgcag tcgctccccg aaagtacacg cgccaagcac gatagtaaaa aacgcctgtg
     1861 cctgtaacac cagcgaagcc agtccagcag gcataccaaa gttaatggcg cagaacagaa
     1921 aggcaaactg cgcaaaactg atggttaatc cataccccaa gagcaaattc agtggtactt
     1981 tcggtcgtgc aacaaaaaag atggccggaa aagcgaccag cataaagcgc aaaccggcca
     2041 gcatcagcgg tggcatgtta tgaagcccca ctttgatgac cacaaaattt agcccccata
     2101 cgaccactac cagtagcgcc aacaccccat cttttcgcga cattctaccg cctctgaatt
     2161 tcatcttttg taagcaatca acttagctga atttactttt ctttaacagt tgattcgtta
     2221 gtcgccggtt acgacggcat taatgcgcaa ataagtcgct atacttcgga tttttgccat
     2281 gctatttctt tacatctcta aaacaaaaca taacgaaacg cactgccgga cagacaaatg
     2341 aacttatccc tacgacgctc taccagcgcc cttcttgcct cgtcgttgtt attaaccatc
     2401 ggacgcggcg ctacgctgcc atttatgacc atttacttga gtcgccagta cagcctgagt
     2461 gtcgatctaa tcggttatgc gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc
     2521 ggttttggta tcctggcgga taagttcgac aagaaacgct atatgttact ggcaattacc
     2581 gccttcgcca gcggttttat tgccattcct ttagtgaata acgtgacgct ggtggtgctc
     2641 ttttttgccc tcattaactg cgcctattct gtttttgcta ccgtgctgaa agcctggttt
     2701 gccgacaatc tttcgtccac cagcaaaacg aaaatcttct caatcaacta caccatgctc
     2761 aacattggct ggaccatcgg tccgccgctc ggcacgctgt tggtgatgca gagcatcaat
     2821 ctgccctttt ggctggcagc tatctgttcc gcgtttccca tgcttttcat tcaaatttgg
     2881 gtaaagcgca gcgagaaaat catcgccacg gaaacaggca gtgtctggtc gccgaaagtt
     2941 ttattacaag ataaagcact gttgtggttt acctgctctg gttttctggc ttcttttgta
     3001 agcggcgcat ttgcttcatg catttcacaa tatgtgatgg tgattgctga tggggatttt
     3061 gccgaaaagg tggtcgcggt tgttcttccg gtgaatgctg ccatggtggt tacgttgcaa
     3121 tattccgtgg gccgccgact taacccggct aacatccgcg cgctgatgac agcaggcacc
     3181 ctctgtttcg tcatcggtct ggtcggtttt attttttccg gcaacagcct gctattgtgg
     3241 ggtatgtcag ctgcggtatt tactgtcggt gaaatcattt atgcgccggg cgagtatatg
     3301 ttgattgacc atattgcgcc gccaggaatg aaagccagct atttttccgc ccagtcttta
     3361 ggctggcttg gtgccgcgat taacccatta gtgagtggcg tagtgctaac cagcctgccg
     3421 ccttcctcgc tgtttgtcat cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta
     3481 aaagggattc gagcaagacc gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca
     3541 ataaagattt aattcagcct tcgtttaggt tacctctgct aatatctttc tcattgagat
     3601 gaaaattaag gtaagcgagg aaacacacca caccataaac ggaggcaaat aatgctgggt
     3661 aatatgaatg tttttatggc cgtactggga ataattttat tttctggttt tctggccgcg
     3721 tatttcagcc acaaatggga tgactaatga acggagataa tccctcacct aaccggcccc
     3781 ttgttacagt tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa
     3841 accggcggtg aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt
     3901 tcctgcactg ataacggatc gttgacacag tagcatcagt tttctcaatg aatgttaaac
     3961 ggagcttaaa ctcggttaat cacgttttgt tcgtcaataa acatgcagcg atttcttccg
     4021 gtttgcttac cctcatacat tgcccggtcc gctcttccaa taaccacatc cagaggctct
     4081 tcaggaaatg cgcgactcac acctgctgtc acggtaatgt tgatatgccc ttcagaatgt
     4141 gtgatggcat ggttatcgac taactggcaa attctgacac ctgcacgaca tgcttcttca
     4201 tcattagccg ctttgacaat aatgataaat tcttcgcccc cgtagcgata aaccgtttcg
     4261 taatcacgcg tccaactggc taagtaagtt gccagggtgc gtaatactac atcgccgatt
     4321 aaatgcccgt aggtatcatt aaccaattta aatcggtcaa tatccaacaa cattaaataa
     4381 agattcagag gctcagcgtt gcgtaactga tgatcaaagg attcatcaag aacccgacga
     4441 cccggcaatc ccgtcaaaac atccatattg ctacggatcg tcagcaaata aattttgtaa
     4501 tcggttaatg ccgaagtaaa agaaagcaac ccctcctgaa aggcgtcgaa atgcgcgtcc
     4561 tgccagtgat tttcaacaat agccagcatt aattcccgac cacagttatg catatgttga
     4621 tgggcagaat ccattagccg aacgtaaggt aattcatcgt tatcgagtgg ccccagatga
     4681 tcaatccacc gaccaaactg gcacagtcca taagaatggt tatccgttat ttctggctta
     4741 ctggcatctc tcgcgaccac gctgtgaaac atactcacca gccactggta gtgggcatcg
     4801 atagccttat tgagatttaa caagatggca tcaatttccg ttgtcttctt gatcattgcc
     4861 actccttttt cacagttcct tgtgcgcgct attctaacga gagaaaagca aaattacgtc
     4921 aatattttca tagaaatccg aagttatgag tcatctctga gataacattg tgatttaaaa
     4981 caaaatcagc ggataaaaaa gtgtttaatt ctgtaaatca cctctgcatt atcgtaaata
     5041 aaaggatgac aaatagcata acccaatacc ctaatggccc agtagttcag gccatcaggc
     5101 taatttattt ttatttctgc aaatgagtga cccgaacgac ggccggcgcg cttttcttat
     5161 ccagactgcc gctaatgttg atcatctggt ccggctgaac ttctcgtcca tcaaagacgg
     5221 ccgcaggaat aacgacatta atttcaccgc tcttatcgcg aaaaacgtaa cggtcctctc
     5281 ctttgtgaga aatcaaatta ccgcgtagtg aaaccgaagc gccatcgtgc atgttttttg
     5341 cgaaatcaac ggtcattttt tttgcatcat cggttccgcg atagccatct tctattgcat
     5401 gaggcggcgg tggcgctgca tcctgtttta aaccgccctg gtcatctgcc aacgcataag
     5461 gcatgacaag aaaacttgct aatacaatgg cctgaaattt catactgact ccttaattgc
     5521 gtttggtttg act
//
LOCUS       DAZFWP020000239         5439 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFWP020000239 DAZFWP020000000
VERSION     DAZFWP020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.241"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1829)
                     /locus_tag="ACHK0Z_RS23730"
                     /old_locus_tag="pgaptmp_004746"
     CDS             complement(<1..1829)
                     /locus_tag="ACHK0Z_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504617.1"
                     /translation="MLQIVGALLLLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVV"
     gene            complement(1950..5072)
                     /gene="agn43"
                     /locus_tag="ACHK0Z_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /gene_synonym="flu"
     CDS             complement(1950..5072)
                     /gene="agn43"
                     /locus_tag="ACHK0Z_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HGU4504618.1"
                     /translation="MKRHLNTSYRLVWNHITGAFVVASELARARGKRGGVAVALSLAV
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKADGTIINQEGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETRINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYSLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFHARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     SSQATLNVTF"
BASE COUNT         1145 a         1557 c         1435 g         1302 t
ORIGIN      
        1 tgaacaacac tgagccggtg aagttgctgg gttgtgacgt agacagccag tgtgcgggtg
       61 aaggtttttc ctgcctggac agcagctgtc tgcagggcag catcgcgatc gccggtgctg
      121 agccagaaga ccgatgccgt cagggcaaaa ctgatccctc cggccgccag actgacaacc
      181 gacccctcgg caatatcata agtgaccgat tcgatggtcc cgaaccgggt gatattacgg
      241 gcctgggtat aagtcagatg tccccgacga atcagccggg acgcttctgc gggatcggtt
      301 acaccgggta ctttaccttc gcggatctta ttcttcatgg tttccacagc gccggcatac
      361 tgatcacggg ggacttccag ttgcatggga ccatgatttc ccatgtaacg atactgtccg
      421 ttttgtccgt cgaatgccgc accgacgcta cgggctgcag ttgaacaata tttcgtctgg
      481 atttctgttc cgctgaccag tctgtctgct ccatttctgg cattattatc accgaggatc
      541 cgtgcgtttt tcagacggac tctgtcgaca atatggtttc cacgttcagc ggcaaaccca
      601 tgtccctgcc ctccggaagc aaacattttc tcatttgtac tccaggtttc cggcagtttc
      661 atgagaatca gaccattcgc cgttgcatcg gaaataaaac cggtgtaaga acgttcataa
      721 atgtcctgac acagtgtttc tgtttcgcga caaagagagg agcagtacca ttccggatcc
      781 ggcttatgat tttccggggc atgatgtact tctgtcagcc cgcaggaggc acagatacga
      841 agctcatcca cgtggtggaa tgatacaaaa cggcggcctt ctgaaaaggg gtaccagtca
      901 ccgccgtcca ggcaacgatc gagaaaatcg tcattcgctt ccggtgataa attcaggcgg
      961 ttccggaaga gggttttcag ttcatcaggg gtaaatgaca ggctctctct cagcttttcg
     1021 ttgaaacccg ccatgtttct ttcataccag gcattgtcca gtagccagtt tccgtctgcc
     1081 agttcaaatt ccctcagttc tccccgggaa atccgttgtg aaatgagtgt cagccagatg
     1141 cgctccgtca gcgtttcagg aaggctgtaa tcgttttgca aactgctcag ccacgattta
     1201 ctgttgagca ccggttgttc ttccagtgtc tggcggatgg cactggtcag tcttttcagc
     1261 gcctggatct ggctggccgc ccatagcagc gtcactaatg cagccagcca gtacaacaac
     1321 ccgccccacc acatcagtga gggtgaaata tcgaactgca atgcgatcaa tggtgcgtat
     1381 gcagaacacg ccataaggaa aaaagcagta taaaaacagc agtaataccc gttaatttcc
     1441 ttgctgatat cttcaggtgt gagcccgttc tgctgaatgc ttcttatctg cacgggaaga
     1501 gccagcagac cagcggcgag agcggtggtc agaataaatt ttacagagtc aaaagtatga
     1561 gcctccagtg cgatatgttt accatcaagt ccccacatga agctgatgat cattccggca
     1621 atcgttagaa atacgcctgc cagccagcga atatggaata accgggttat gcgttcagtg
     1681 atatagccag ccagtaaagc cgggccgaac agacacagca atacgaatcc tgccagcgca
     1741 gacgccgtgc tggttaatgc tctgaacaaa aggcgaagaa tggcaaatcc tgcgatcagc
     1801 aggagcagcg cgccgactat ctgtaacata gtcactccgt gatgaatatc atgttattgt
     1861 gaatgccagt gaatgtggca ctgaaacgtt ttcccccgtc cgggtcccgg tcatgatgac
     1921 cgggaccaca gagaggcaat gcagaattat cagaaggtca cattcagcgt ggcctgactg
     1981 ctataacctt cagcgctgct gccgttgatg ctgtgggcat aaccggcctg aacgcccagg
     2041 gtgatatttt cccggacacg ggcttccagt ccggcctgca gatccagtga agtgccattc
     2101 tgtgagggag agaacgtcat cccgctgcct gcggtggagg tccccacacg catatcaccc
     2161 cgggagctga aggtgcggat aacggaaggc tgtacccacc agttcaccgg cagttcacgc
     2221 acactgtgtt ttgcactgtc acgcaggggg gcacgggatg aggtgccttc accaaaggtc
     2281 atatcgttgt ggctgcccag acggaaaccg gcacgcacat gctgtgcact gccatgcccg
     2341 aacttcacat aaccggcgtt gtccttaccg tcatccagcg aaagcccctg ccaggtgtac
     2401 tgcagccgtg gctccagcat caggttatca gtgatactga agggcagacc ggtttccagt
     2461 gagcccagcc agccccggcc ccgtgcgtgg aagtcgttat tgcccgatga cgctttcata
     2521 ctgtggcggg ttccctgtgc cacaatgtca gcccacaggc cggaggaggt gtgtgtcagg
     2581 ttcatatatc cgcccaggct gccggcatca tcccggaccg tgccggcgcg ggagccgtca
     2641 tcatccttaa catcaacgga agaatggcct gcagcaccat atacccctgc ggtcacagac
     2701 ataccggcaa cctctgttct cagcaggtca ccctccaggc ggacgaagcc atagctgccg
     2761 ctgctttccg gcgtggcccc acgggcaata ccgccgttgt tatcgtgacc gagatggcca
     2821 ccctgaatgc tgagacggac gctgttattt tcaccgttta caccggtctg atggctgcgg
     2881 gagcctgcca gaatccggtc atagtccatt gcctgtgtca gcatggaggc gtacaggggg
     2941 acttcagcac gataacgttc ttcactgcgc agataccagc tctcatcact gtcccggttg
     3001 agggagtagt taaaggcccc ggcctgcagc atattcccct ggacaaaggc cccttcctcc
     3061 gtggtggcac cgttaatggc ctcaaccacc tgaataccct taccggtggt cgccagcccc
     3121 gtaccactgt tgccggcgtt caccaggtta aggatggttt ttccggttgc cctgccaccg
     3181 tcaatgacca gtctgtcagc attgttctgt gccatatccg ggcgtacacg caggctgatg
     3241 gtgccattct gcccgttcag gtttttcacc tgcagggttg tcggaacgaa cttccctgtg
     3301 cgggcggagg tgaaatgaat ctgtccggca tggctgaggt catccaccac cgactgaacc
     3361 gtggcgttat cggggatatt ccaggtggca ccggatgtca gggtgacatt cgtggggtca
     3421 atggcaccgt tcagcactgt gctgccggtc agcttcaggg ccgtgccgcg ctgagcgatg
     3481 acatccgtgg tgacggtact gtcgttcagc gtcagcgtgc cttcattcag gttgacggtt
     3541 ttctgggtga gtgtggtgtt gctgacagtg agtgtgccac ttcctgattt ttccaccctg
     3601 ccgttaccgg taagagcgcc tccctgcagg agtgcatcac cttcacggat ggtcagggta
     3661 tcgttattca ccgttttccc ggaaagggta agtatggcgc cgttattcag cgtggtggtg
     3721 cccgccagtg tgccgcccct ggcggtgaac agtccgccat ttaccgtggt atccgtggcc
     3781 gtatcaccgg cgttcagcgt gaatgaactg cctttttcca gcatcagggc atccgcctga
     3841 ccgcctccga tacggaatgc ctttccgtcg ctccgggtac cactgacagc ggcaccggaa
     3901 tcggccagca gtacaccgcc attccccatg gatacggtgg tggcggcacc accgttgcgg
     3961 acatccagcg ttccgccatc atccacacgg gtattagtgg ctgtgtgtcc gctcagcaca
     4021 tccagacgtc cgccattttc cagtacgaca ttatcagcct tacccgccac aacggagaat
     4081 gctcccaggc ggtttgtgcc ggtgacggtt gcagctgtac tggtaaccag tgcaccgccc
     4141 gtgttctggg tgacatcaga cgctttaccg ccggcattca cctgcagctt tcctttctgg
     4201 ttgatggtgg tatgcgcggc agttcctcct tccttaatca cctgccagcc atcacggttt
     4261 atccgcgttt ctgttgccgt gccaccgttg tgtacatact ggttaccccc ctccagccgg
     4321 gtgttatgtg cctctccgtg taccgtctgg tcaccaccgg cgtaaatgag ggtgtcccgt
     4381 gccatccccg aagaccagat aacctgccgg ccatttttgt tgatggtggt ggattcagcc
     4441 gtccctccga ccatctgacc gctggacaca ttttcagact ccggaccacc ttctgcaccg
     4501 gtgttgacga tggttccggt tgccagtccg ccatgtttta tggtctggta accctcctgg
     4561 ttaataattg taccgtcagc tttccctccc ccgtgtaccc actgctcacc tctgttatcc
     4621 agcgtggtgt tcaccgccag tccgttaagg ctctgccctc cgccatcacg aataaccgta
     4681 tcactggcag ttcctcctgc ctgcacaatc tgacgaccat ttgcggtgac agtggtgttt
     4741 ctgcctgtgc cacccgcttt tatccattgc ccgccggtgt tttcgtcact gtccggcccc
     4801 agctcaagcc cggtactgac agtcacgcca tcagctgttc cggatacaaa ctggttgtca
     4861 tggtttacca gtgttccgcc attcactgtt tcacccgggt gcacaacgat gtcagcagcc
     4921 agcaccggga gtgacgtgac cacggcaaga gacagtgcaa ccgccacacc gccacgttta
     4981 ccccgtgcgc gggccagttc ggaggcaacc acgaaagcgc ccgtaatgtg attccatacc
     5041 agcctgtagc tggtgttcag atgtcgtttc atcagctttt ccttacagag ggtgaataaa
     5101 aaagccggta cccacaaatg tggataccgg caggtacaaa acagcatggt cagataaagt
     5161 ggtttttccc ggacataaat gcttccactc tctcatccgc cagagaagta tcagtccatt
     5221 gtgtgcaatt ccgttctgca tgatgaagag attcagctgc ctttttgcag gccattaacc
     5281 actataaatg atcgatatca acgatcgata cctgttttat cgatcgtttt attctattaa
     5341 cggcaagaca gtcatgcaag agaaagaggt gggtatgagg gggttgtgtc atgttttcag
     5401 ttggttaacg caatgtaatc aggcatttcc cctggcaga
//
LOCUS       DAZFWP020000240         5380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFWP020000240 DAZFWP020000000
VERSION     DAZFWP020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.242"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..1353
                     /gene="garP"
                     /locus_tag="ACHK0Z_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             19..1353
                     /gene="garP"
                     /locus_tag="ACHK0Z_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU4504619.1"
                     /translation="MILDTVDEKKKDVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAHLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1369..2139
                     /gene="garL"
                     /locus_tag="ACHK0Z_RS23745"
                     /old_locus_tag="pgaptmp_004749"
     CDS             1369..2139
                     /gene="garL"
                     /locus_tag="ACHK0Z_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU4504620.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2169..3059
                     /gene="garR"
                     /locus_tag="ACHK0Z_RS23750"
                     /old_locus_tag="pgaptmp_004750"
     CDS             2169..3059
                     /gene="garR"
                     /locus_tag="ACHK0Z_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4504621.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3156..4301
                     /gene="garK"
                     /locus_tag="ACHK0Z_RS23755"
                     /old_locus_tag="pgaptmp_004751"
     CDS             3156..4301
                     /gene="garK"
                     /locus_tag="ACHK0Z_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU4504622.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4334..4710
                     /gene="rnpB"
                     /locus_tag="ACHK0Z_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     ncRNA           4334..4710
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK0Z_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5218..>5380)
                     /locus_tag="ACHK0Z_RS23765"
                     /old_locus_tag="pgaptmp_004753"
     CDS             complement(5218..>5380)
                     /locus_tag="ACHK0Z_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504623.1"
                     /translation="EELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYT
                     YNYDLLNAI"
BASE COUNT         1265 a         1276 c         1523 g         1316 t
ORIGIN      
        1 gtacccgagg taaataaaat gattctggac accgttgacg aaaaaaagaa agacgtgcat
       61 acccgctatt taatattact gattattttt attgttaccg ccgttaacta cgccgatcgt
      121 gcaacgctgt ctattgctgg taccgaagtg gcaaaagagt tgcagttaag tgcggtttcg
      181 atgggttaca tcttttctgc ttttggctgg gcccacttgc tgatgcaaat ccccggcggc
      241 tggctgcttg ataagtttgg ctcgaaaaaa gtttatacct atagcctctt tttctggtcg
      301 ctattcactt tcctgcaagg ctttgttgat atgttcccgc tggcctgggc agggatctcc
      361 atgttcttta tgcgctttat gctcggcttc tcggaagcgc catcattccc ggcgaacgcc
      421 cgtattgtcg ccgcctggtt cccgacgaaa gaacgtggta ctgcctccgc catctttaac
      481 tcggcgcaat atttctcgct ggcgctcttt tcgcctctgc ttggctggct gactttcgcc
      541 tggggctggg agcacgtctt taccgttatg ggggtgattg gttttgtgct gacggcgctg
      601 tggatcaaat tgattcataa cccgacagat cacccacgta tgtctgcgga agagctgaag
      661 tttatctctg aaaatggcgc ggtggtcgat atggaccaca aaaagccggg cagtgcggca
      721 gcaagcggtc ccaaactgca ttacatcaag caattgctct ctaaccgcat gatgttgggc
      781 gtatttttcg gacaatattt tatcaacacc atcacctggt tcttcctcac ctggttcccg
      841 atttatctgg tgcaggaaaa aggcatgtcg attctgaaag tgggtctggt cgcctcgatt
      901 ccggcactgt gtggttttgc gggcggcgtg ctgggaggtg tcttctcgga ttatctgatc
      961 aaacgcggtt tatccctgac cctggcacgt aagctaccga ttgtgctggg aatgttgctg
     1021 gcttccacca tcatcttatg taactacacc aacaacacca cgctggtggt catgctaatg
     1081 gcgctggctt tttttggcaa aggatttggt gctctgggct ggccggtgat ttctgacacc
     1141 gcgccgaaag agattgttgg cctctgtggc ggcgtcttta acgtctttgg caatgttgcc
     1201 tccattgtca ctccactggt gattggctac ctggtaagtg aactgcactc cttcaatgca
     1261 gcactggttt tcgtgggatg ttcagcgctg atggcgatgg tctgctacct cttcgtagtt
     1321 ggcgacatta aacgtatgga attgcagaaa taagcaaagg tataagcgat gaataacgat
     1381 gttttcccga ataaattcaa agccgcactg gctgcgaaac aggtacaaat tggttgctgg
     1441 tcagcactct ctaacccgat tagcactgaa gttcttggtt tggctgggtt tgactggctg
     1501 gtgctggatg gcgaacatgc gccaaacgat atctccacgt ttattccgca gttaatggcc
     1561 ctgaaaggca gcgccagcgc gccagtagtg cgagtgccga ccaacgagcc ggtaattatt
     1621 aagcgtcttc tggatatcgg tttctataac ttcctgattc cttttgtaga aacaaaagag
     1681 gaagcagagc aggcggtggc atcaacccgt tacccaccgg aaggcattcg cggcgtctcc
     1741 gtttctcacc gcgccaatat gtttggcacc gtggcggatt atttcgctca gtcgaacaag
     1801 aacatcacca ttctggtcca gatagaaagt cagcagggcg tagataacgt cgatgccatt
     1861 gccgctaccg aaggtgtaga cggtatcttc gtcggcccca gcgatctggc tgcggcatta
     1921 ggccatctcg gcaatgcctc gcacccggat gtacaaaaag caattcagca catttttaac
     1981 cgtgccagtg cgcacggcaa acccagcggt attctcgcgc cggtcgaagc cgatgcgcgt
     2041 cgttatctgg aatggggcgc gacgtttgtg gctgtcggca gcgatcttgg cgtcttccgc
     2101 tctgccactc agaaactggc tgataccttt aaaaaataac caccaccgca acaagagaga
     2161 tgattgatat gactatgaaa gttggtttta ttggcctggg gattatgggt aaaccaatga
     2221 gtaaaaacct tctgaaagca ggttactcgc tggtggttgc tgaccgtaac ccagaagcta
     2281 ttgctgacgt gattgctgca ggtgcagaaa cagcgtctac ggctaaagcg atcgctgaac
     2341 agtgcgacgt catcataacc atgctgccaa actcccctca tgtgaaagag gtggcgctgg
     2401 gtgagaatgg catcattgaa ggcgcgaagc caggtacggt attgatcgat atgagttcta
     2461 tcgcaccgct ggcaagccgt gaaatcagcg aagcgctgaa agcgaaaggc attgatatgc
     2521 tggatgctcc ggtgagcggc ggtgaaccga aagccatcga cggtacgctg tcagtgatgg
     2581 tgggcggcga caaggctatt ttcgacaaat actatgattt gatgaaagcg atggcgggtt
     2641 ccgtggtgca taccggggaa atcggtgcag gtaacgtcac caaactggca aatcaggtca
     2701 ttgtggcgct gaatattgcc gcgatgtcag aagcgctaac gctggcaacc aaagcgggcg
     2761 ttaacccgga cctggtgtat caggcaattc gcggtggact ggcgggcagt accgtgctgg
     2821 atgccaaagc gccgatggtg atggaccgca acttcaagcc gggcttccgt attgatctgc
     2881 atattaagga tctggcaaat gcgctggata cttcccacgg cgtcggcgcg caactgccgc
     2941 tcacagctgc ggttatggag atgatgcagg cactgcgagc agatggttta ggaacggcgg
     3001 atcatagcgc cctggcgtgc tactacgaaa aactggcgaa agtcgaagtt actcgttaat
     3061 gacgtcgtgc ccggatggcg tattgcaatc cgggcctgga atccaggccg aataagagaa
     3121 acgccctccg gcgtcatgtg gtaacaggca taggtatgaa aatcgtaatc gccccagact
     3181 cttataaaga aagtttatct gccagcgagg ttgcgcaggc gatagaaaaa ggatttcggg
     3241 aaatttttcc tgatgcacag tacgtttcta ttccggttgc cgacggtggc gaaggaacgg
     3301 tggaagcgat gattgcagcc acccagggtt ctgaacgtca cgcctgggtt acagggccgc
     3361 tgggcgagaa agtgaatgcc agttggggga tctccggcga tggcaaaacc gcgtttattg
     3421 aaatggcggc ggccagtggg ctggagctgg tacctgcgga aaaacgcgat ccactcgtga
     3481 ccacttcacg cggcacaggc gagttaatcc tgcaggcgct ggagagcggc gcgacaaaca
     3541 ttattatcgg cattggcggt agcgctacaa atgatggcgg cgcaggcatg gtacaggcgc
     3601 tgggggcgaa attatgcgac gccaacggca atgaaattgg ttttggcggc ggtagtctta
     3661 atactctgaa tgatattgat atttccggcc tcgatccgcg cttaaaagat tgcgtcattc
     3721 gcgtcgcttg tgatgtcacc aatccgctgg tgggcgataa cggcgcatcg cgcatctttg
     3781 gcccacaaaa gggagccagt gaagcgatga ttgttgagct ggacaataac ctctctcact
     3841 atgccgaggt cattaaaaaa gcgctgcatg ttgatgtgaa agatgtcccc ggcgcaggag
     3901 ctgcgggcgg tatgggtgcg gcgctgatgg cgtttcttgg tgcggaactg aaaagtggta
     3961 ttgaaatcgt cactacggcg ctgaatctgg aggaacatat tcacgattgt acgctggtga
     4021 tcaccggtga agggcgtatt gacagccaga gtattcacgg caaggtaccg attggcgttg
     4081 caaacgtggc gaagaagtac cataaaccgg tgattggcat tgcgggtagc ctgaccgatg
     4141 atgttggcgt tgtacatcag catggcatcg atgcggtctt cagcgtattg accagcatag
     4201 gtacgttgga cgaagcattc cgcggggctt atgacaatat ctgccgtgct tcacgtaata
     4261 tcgccgcgac actggcgatt ggaatgcgca acgcggggtg acaagggcgc gcaaaccctc
     4321 tatactgcgc gccgaagctg accagacagt cgccgcttcg tcgtcgtcct cttcggggga
     4381 gacgggcgga ggggaggaaa gtccgggctc catagggcag ggtgccaggt aacgcctggg
     4441 ggggaaaccc acgaccagtg caacagagag caaaccgccg atggcccgcg caagcgggat
     4501 caggtaaggg tgaaagggtg cggtaagagc gcaccgcgcg gctggtaaca gtccgtggca
     4561 cggtaaactc cacccggagc aaggccaaat aggggttcat aaggtacggc ccgtactgaa
     4621 cccgggtagg ctgcttgagc cagtgagcga ttgctggcct agatgaatga ctgtccacga
     4681 cagaacccgg cttatcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt
     4741 tttgcttttg gaggggcaga aagatgaatg actgtccacg acactatacc caaaagaaag
     4801 cggcttatcg gtcagtttca cctgatttaa cgaaaacccg ccagcgtaaa ccggcgggtt
     4861 tttgcttttg gaggggcaga aagatgaatg actgtccacg acgctatacc caaaagaaag
     4921 cggcttatcg gtcagtttca cctgatgtac gtaataaacc gttccggcgg gtttcagatt
     4981 gttgagtgcg ctttattcat gccggatgcg gcgtgaacgc cttatcctgc ctacaaaagc
     5041 acgcaaattc aatatattgc agagatggcg taggcctgat aagcgtagcg catcaggcat
     5101 ttttgcggtt gccatcagtt tcaaacccgc gtcagtgagt ttttgctttt ggtgcacagg
     5161 gtaagatgaa taaatatcta cggtattaat aatagacttt gtaagaagtt attaagtcta
     5221 tattgcattt aaaaggtcgt aattatacgt atagtcctct ctggtaaaat attgattctc
     5281 tgagttatat gcttcatatg tgttatattc cccgacctca aaaccattgt ccggagaatt
     5341 ttcgtctttc tcgagggtgt tatctgaaag taactcttct
//
LOCUS       DAZFWP020000241         5305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFWP020000241 DAZFWP020000000
VERSION     DAZFWP020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.243"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..64
                     /locus_tag="ACHK0Z_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             <1..64
                     /locus_tag="ACHK0Z_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504624.1"
                     /translation="TLLQQPEERFAWLRSKKDRF"
     gene            136..1494
                     /gene="mltD"
                     /locus_tag="ACHK0Z_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             136..1494
                     /gene="mltD"
                     /locus_tag="ACHK0Z_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU4504625.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSSVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1542..2312)
                     /locus_tag="ACHK0Z_RS23780"
                     /old_locus_tag="pgaptmp_004756"
     CDS             complement(1542..2312)
                     /locus_tag="ACHK0Z_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4504626.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTLEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2390..3190)
                     /gene="yafD"
                     /locus_tag="ACHK0Z_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             complement(2390..3190)
                     /gene="yafD"
                     /locus_tag="ACHK0Z_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4504627.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3431..4345
                     /gene="yafC"
                     /locus_tag="ACHK0Z_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             3431..4345
                     /gene="yafC"
                     /locus_tag="ACHK0Z_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU4504628.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4342..5145)
                     /gene="dkgB"
                     /locus_tag="ACHK0Z_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             complement(4342..5145)
                     /gene="dkgB"
                     /locus_tag="ACHK0Z_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU4504629.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1397 a         1278 c         1313 g         1317 t
ORIGIN      
        1 aacattattg caacaacctg aagaacgttt tgcatggtta aggtcaaaga aagataggtt
       61 ctgataaaac tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca
      121 actattgaca cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt
      181 tgccagagta ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg
      241 gaagcagcaa agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca
      301 gatggtgact tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac
      361 cggattcgcg aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact
      421 ttacgggcag agccgtatat gtactggata gccgggcaag ttaaaaaacg taacatgcct
      481 atggaactgg tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc
      541 gccaatgccg caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa
      601 cagacccgca attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaac
      661 atgatgcagc gtctgaacaa gatgtttgac ggcgactggc ttctgaccgt agcggcttat
      721 aacagcggcg aaggtcgggt catgaaggca attaaaacga acaaagcgcg tgggaaatcc
      781 acggacttct ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg
      841 gcattgagtg atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat
      901 gaaagccgtg ctctggcgcg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca
      961 gatatggcgg ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc
     1021 acgctgggcg caagtggtcc gcaatacgtg atggtgccaa agaagcatgc agatcaactg
     1081 cgtgaatctc tcgcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat
     1141 acgccgctta acagcagtgt ttacaccgta cgctctggcg acacgctttc aagtatcgct
     1201 tcacgtctcg gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag
     1261 ctgaagccag gccaaagttt gacgattggt gcaggcagta gcgcacagcg actggcaaac
     1321 aacagcgata gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa
     1381 cgccacggcg tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa
     1441 ccaggcgaca agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc
     1501 agataataaa aaggcaccga ttcccccggt gcctttttta tttatgctgc tttatgtgct
     1561 tctaccatga tgatatcact ggtgaaagag ccatcattct gcaaggcaaa atacgttctc
     1621 acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc ttctagcgta
     1681 cgcattctcg cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca
     1741 actatcagat tggcttcatt gattaacctc aaccactcac cgctggcgta gtttcgtacg
     1801 tgagaggtat cgcgtaatgc ttctaccgtc tgtaaccaga tgtcgcgcac tggatgaccc
     1861 ggagacatta cgtccatcac aatcatccta ccgccaggtt tcaatatcct attcacttct
     1921 cgcagtgctg caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca
     1981 aatgcgttat cggcaaatgg cagactttcg gcataaccct ggcgggtggt gatatttttc
     2041 agttgccggg cttcggcagc ttgtgccacg acatccagca tttgggcaga taagtcatac
     2101 gccaccaccg cgctcacgtt ctgcgcagcg acaaagctgg catgccctgc tccgcagccc
     2161 atatcaagta cacttgcatc aggataatca gccagacaca ccgccaggcg ctgtaaatct
     2221 cgcccggatg catgtacggt actggttaaa tactcactgg cctgcgagct aaactgcttt
     2281 tctacgtggt catggtggga ttgtgttgtc attgcgctgt ccttttgttg tttgagtata
     2341 aaaggacagc gccaaacgtt ggtctgccct gtggcagacc tgacatacct tatttatcag
     2401 gcttgccggg actgaattca acgagtagcg gattgtgatc ggaagcgcgc gtaaccaata
     2461 cagaagcttc gctgacgttc agaccacggt agaaaacaaa atcgagcggg cggccaaacg
     2521 cccgacggcg ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc
     2581 gatataacgc gttcatcctt ctacggctcc aggcattgaa atctcctgcc ataatgaccg
     2641 ggccgctgtg gtgagctatc tgatcaccaa taggaagtaa ctgcttacta tagacatcca
     2701 cgcccagact gaagttgacg gcgtgtatat taaccaccat caacaggcgg gtgtcaggta
     2761 atggatagac cgtcaccagt gccgacttcg ccagacgcaa aatgggttct cgttcacgta
     2821 acgggcagca atacactgga tgcgccgccg aaagggtcat tacgccagaa ggatgttgtg
     2881 gcagtacgaa agcgggtacc tgatcggcgg caagatagtt agcggtcgca aactgtacta
     2941 actctggcgt tgtctgcgct tcctgcaata acaccagatg tgcatctttg ccgtagttct
     3001 ttaataccga caaccattca gcgcgttgct gtttgtatat gttccacacc aggatccgaa
     3061 tacgctcttc ggtacttaac ggttctccgg gtggtaatgc ctggccgatg ctcgcaaaag
     3121 accccggcgg taagatcctt tccgcaggtt gtccggcaac atagcgcatg gcataggtgt
     3181 tttttcgcac ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt
     3241 tctactgtta tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa
     3301 gcaagtgctt acaccggtca gaattttgct cattatggag aacattaaat cgcatgacat
     3361 atcacgcatc ctgatggttt aatgacaaat aaattcaaaa tatctcttct attttgcaac
     3421 aggagcaaaa atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtcgaaag
     3481 cggcagcttt agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc
     3541 ggtgaaaaag ctggagatga aacttggcgt tagcctgctt aatcggacca cgcgacaact
     3601 tagcctgacg gaagaaggcg agcgttattt tcgtcgcgta cagtcaattt tgcaggagat
     3661 ggcatcggca gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttacggat
     3721 cgatgccgca actccagtgg tgctgcactt tctgatgccg ttaattaagc ctttccgtga
     3781 acgctatccg gaagtcactt tgtcgctagt ctcctccgaa acgattatta atttgatcga
     3841 aagaaaagtg gatgtcgcga tacgcgctgg tacgttaacg gattccagct tacgtgccag
     3901 gccgttattt aacagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg
     3961 gaagccagaa acgatcgacg atttaaagca acatatttgc ctgggattca ctgaacccgc
     4021 ttccctcaat acctggccga tagcccgtag cgatggacaa ttacatgagg tgaagtacgg
     4081 tttgtcatcc aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc
     4141 gtgtttgtcc gactacatga tcgacaaaga aatcgctcgc ggagaattgg tggagttaat
     4201 ggcagataaa gtgttgccag tggaaatgcc attcagtgca gtctattaca gcgaccgtgc
     4261 ggtaagtacg cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc
     4321 cggaggagct gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag
     4381 gcggtcgttg caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt
     4441 ttgtgcctta agattacttt ccaggttttc acgtttagta gaagaaggaa ttactgagta
     4501 accttccccc atagcccacg ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc
     4561 gatacgagca ataacctcat ctttcagggc cttaccatac gccagcgtca tataggaagt
     4621 aatatggatg ccgtgctgtt tagcccagac aaccacttta cggttttgca gataaggaga
     4681 gagttcaatc tggttagtag cgatgttttc agcgccaaca gcagcaatcg ttttttccat
     4741 caacgggatc gtgaagttgg aaataccgat ctcacgtgtc agaccttgtt ttttggcttc
     4801 cagcagcgcc tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat
     4861 tagcgtcaga tcaacataat cggtacgcaa tttttgcagg ctctctttca gacttgggat
     4921 caatttgtct ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg
     4981 tggcacgcca ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc
     5041 ggtatcaatt gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc
     5101 gtctttcaga cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta
     5161 atgctatttt cgttaactga ggtgattatg ggcttcagac tgttgcggaa aaagagcgta
     5221 aaacgcagag gatttttgcg attctggcaa taatagaaat gtagacgaaa aaaaacctcg
     5281 agcgataact cgaggcttct taata
//
LOCUS       DAZFWP020000242         5255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFWP020000242 DAZFWP020000000
VERSION     DAZFWP020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.244"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            337..459
                     /locus_tag="ACHK0Z_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /pseudo
     CDS             337..459
                     /locus_tag="ACHK0Z_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(774..968)
                     /locus_tag="ACHK0Z_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             complement(774..968)
                     /locus_tag="ACHK0Z_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504630.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIFTAL
                     LALLVVVLLVTSAVSMYVLW"
     gene            1168..1737
                     /locus_tag="ACHK0Z_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             1168..1737
                     /locus_tag="ACHK0Z_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGU4504631.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            1905..2288
                     /locus_tag="ACHK0Z_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             1905..2288
                     /locus_tag="ACHK0Z_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4504632.1"
                     /translation="MYAKSFLALDGNGRLTGARTAHTAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERKEIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            2285..2710
                     /locus_tag="ACHK0Z_RS23820"
                     /old_locus_tag="pgaptmp_004764"
     CDS             2285..2710
                     /locus_tag="ACHK0Z_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4504633.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            2997..3152
                     /locus_tag="ACHK0Z_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /pseudo
     CDS             2997..3152
                     /locus_tag="ACHK0Z_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(3401..3544)
                     /locus_tag="ACHK0Z_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /pseudo
     CDS             complement(3401..3544)
                     /locus_tag="ACHK0Z_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3573..5158)
                     /locus_tag="ACHK0Z_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /pseudo
     CDS             complement(3573..5158)
                     /locus_tag="ACHK0Z_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5189..>5255)
                     /locus_tag="ACHK0Z_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     CDS             complement(5189..>5255)
                     /locus_tag="ACHK0Z_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4504634.1"
                     /translation="LLDKLDWRQPKTSRLNALTML"
BASE COUNT         1194 a         1371 c         1384 g         1306 t
ORIGIN      
        1 tcatatctgt gaaataaaca ctgtgagtgt gatcagtggg catgagaacg cagtagcaac
       61 agattacgtt gtactcacag ggctatccct gatagctggc gtataacctg cgtatataaa
      121 acgtacctgc aacacacgtg aacagagtgc attaagcatc cgtctgagcg gtaaacgtat
      181 ccgacctggt gagtgacagt ctgagagacc ggttaagtgg tggtacggga gtaatgagtt
      241 tggggtgggt accagaatgc cagtctggtg cggtatgctg gcagtatcac gttaattgca
      301 gcataaagtg atgagaggct aagtgatgag aggctaatgg aagttaaaac caaagaagac
      361 tggctgtatc agttccgtcg ttgttcatcc cgggagacac tggaaaaagt gattacccgc
      421 acacgttata aacttacccc tgcggagctg gaagccttct gcggtcgatc ggctggcaga
      481 actgacgaca aactttacgc gtgtggagcc agtctggtca cctgagtatc cggtaatgac
      541 aacggtctgg tgttatctgc tctgtgtgat tctttttagg tcttctccgg ttgttggtaa
      601 cctggataat accgcgaacg ttaagcggta ttgtgacaac gaataatttg aaattaaaag
      661 gggcaggggg gggctgcttc gggtattaac ggcttacagc gtgtgggttg acggcgcgtt
      721 gcaggggacg aactctggca accctcacgg atatctgata agccccgtta ccgctaccac
      781 agcacataca tgctgacagc agatgtcacc agcaaaacca caaccagcag cgccagcagg
      841 gctgtgaaga tcctgtccgt attccggtaa tagccgaagg ggtcgacaga accacagtac
      901 ggacaacaca ccgtattatg agtaatcgcg ttaccacagt ccctgcactt ctttttgcgt
      961 atcaccatca ctccctcctc acagccttac ccgtaaccgg ttttttacat taaaaaaaca
     1021 acgctgaaaa ttcaaccgtc agtccggaga cgaccgttcg ggttatcaca aagtgcctga
     1081 gacagtgtcc tgccggaggt cacttcaggg actctgcgca ttttttacga cgtggctatt
     1141 ccgtatgaac catacggaga tttaaccatg acctacaaat acaacccctt ctggcagcaa
     1201 cgtattcgtg agacggtgcg gcacgcactg aatgttcatc cccgcctgac ggcattgcgg
     1261 gttgatctgc gtttcccgga tgtaccggca gcaacggacg cagctgtgat atcccgcttc
     1321 atcaatgccc tgaaagcccg aatcgacgct taccagaaac gtaagcatcg ggaaggtaaa
     1381 cgcgtgcatc ccaccactct gcattacgtc tgggcccggg agtttgggga gtgcaaaggt
     1441 aaaaaacact atcatctgat gctgctggtc aaccgggata cctggtgtcg tgccggtgat
     1501 taccgcgctc cgggatcact ggccgggatg attaaacagg cgtggtgcag tgccctggga
     1561 gtggataccg gagatcatac cacgctggcg cattttccgg tcaggccggc ggtgtggctg
     1621 gaacgcgatg atgacaccgg ctttcagcag gtgctggagc gtgctgacta tctggcgaag
     1681 gagagtacca aagcttacgg caccggtgag cgcaactttg gctgcagtcg tggctgagcc
     1741 cgacagacga cttatctgac cttagtgtga tgacgtgttc acagcgacca cactgatttt
     1801 cccttctgag actgaactga aaaccgccat tcacacctga ccacgctgcc cgtaccggga
     1861 cgactgttgc ctgcgtgtcg tctggcggct gaggaatata tactatgtac gcaaaatcct
     1921 ttctcgcact tgatggtaac ggacgtctga cgggggcccg taccgcacat actgctcctt
     1981 atgcccacta cacctgccac ttgtgtggca gtgcactcag ataccacccg caatacgaca
     2041 ccgaactgcc ctggtttgaa cacactgacg acgggctgac agagcacggt caacagtgtc
     2101 cttatgtcag accggagcgc aaggagatac aactgattaa acgtctgcag caattcgtac
     2161 cggatgcctt acccgtagta cgtaaagcca gctggcactg caggcaatgt caccacgatt
     2221 attatgggga gcggtactgc acacactgcc agaccgggcg cttcagtgag gaggggggag
     2281 cagaatgaac ggtataaaaa cggggctggg gatcacgccg ggtgagcaca ttattagtgc
     2341 cgactctgcg ctcagcagaa atatccggca gtgtttctgc ctttcctgtc atggccggtt
     2401 gattctgcag acagatgctc agggggcgtg gtttgagcac gatttacatg ccctgagcga
     2461 gcaacagaag gcggactgtg tggtgctgaa tccggagaag tcccaccctt acattgagga
     2521 tatggcgatg tttttatcgc cattaccggt cgtgctggag tggcattgtg tgatgtgtga
     2581 gcagtttttt catgggaaga agttctgtga ggcctgtggt acggggattt attgcagagc
     2641 ggtatgtacg aagtctgttt acagctacca gccagacttg tttgaggact gcggaggtgc
     2701 gagtacttaa tccgaaatct ccgctctgcg cagggcgttc agaggtatcg caacctgcat
     2761 tttatccggt gtttcgcacc tgccccgttc gtgacacaaa ggcgcgacac cggatatccg
     2821 cagtcctgct tacctgaata aaacccgggg tattgattgg tagtgtggtg cggaatcgta
     2881 ccgtgatctc gcattcgccg aattcgcact gtggtgtcaa tcaaccatac gcgagagtcc
     2941 tctcgtattt atcagtgcct caggacgatt tctgcatagt taatccaggg ggctgcattg
     3001 ttcatttccc tgctaatgga cgtgacatcc tggatattaa cgatcctgat ttccctgagc
     3061 agtatcagcg cttattggca cgtctggatt atctgacgaa gctcgacact aaagcatcag
     3121 gccagcgtaa ctctggctac agcggttttt gacagttgta actgtaaggt gatttattat
     3181 gtatacagaa tcaaatgtta agctttcctg taacgccctc tggcaaaagt gtgtccttgc
     3241 aactctgtat cgggctgcag ctacacatcc tgaaattaaa ttattacaat ttaaattaat
     3301 accagggcag ggcattaata aggccattcc ctctctgatg gctgatttta ctggtgaact
     3361 gcatagactc atttctgaat atttgcagtg caagacagtc cgggctgaga ccgccacagg
     3421 cactgtgacg acgccagcga ttgtaatcac actcgatata attaaacacc gtttcccgca
     3481 ttatttcccg gctgctaaag cgttccccgt ggatacattc caccttcagc gaatgaaaga
     3541 aggtaagcgt caacggagca ccgtattgac gcttatttat tggtgagtac tacgttccat
     3601 ggcaggagtt catcaacccg gttggagggc cattccggca gtacgctcag aatatggcgc
     3661 agatacgctt ccggatcgat accgttcaga cggcaggtgc cgatcagccc gtatagcagt
     3721 gcaccacgct cgccgccgtg atcgctaccg aagaacacgt aatttttctt tccaagacag
     3781 actgcacgaa gcgctctttc cgctgtgtta ttgtccgtct ccgccagtcc gtcatcactg
     3841 taataacaga gggcgtccca ctgattcagt acatagctga acgcttcgcc cagtctggat
     3901 tttttcgaca gcgtgccatt cttctccatc atccattcat gcagcgacgt cagtaacgct
     3961 ttgcttcgct gctgtctgac tgcaagacgc tctgactctg gtaatccccg tatttcatcc
     4021 tcgatggcgt acagttcact gattcgcttc agggcttctt ctgccgtcgc acttttgctg
     4081 ctgatgtata catcgtggat ttttctccgg gcatgggccc agcacgcaac ttctgtcagc
     4141 gcaccacctt cacgttctgc actgaacagc ctgtcataac ctgtgaacgc atccgcctgc
     4201 aggatacccc ggaaggggcg gaggtgttgc tccgggtgtt tcccctgccg gttcggtgag
     4261 tacgcgaacc agaccgccgg aggagatgac gaacccgcat tgcgatcatc ccggacatac
     4321 gtccagatgc gccctgtttt cgtctttttc aggcccggtg ccagtacctt taccggtgtg
     4381 tcgtcagtgt gaagcttgcg ggtattcatc acataacggt acagggcatc attcagcggc
     4441 gtcattaact ggcagcacgc gtcaacccag ttggagagta atgcacggct cagttcgaca
     4501 ccctgtcggg caaagatttc actctgacga tacagtggca ggtgttcgca gtattttccc
     4561 gttaacacgc gggcaagtaa tccggggccc gcgataccac gctctatcgg gcgggacggc
     4621 gccggtgctt caacgatgca gtcacatttt gtacacgctt tttttacccg ttctgtgcgg
     4681 atcactttca gggcgctgct caccagttcc agttgttctg cactgacttc ccccagataa
     4741 tccagctcac cgccacactc cgggcaacag ctttcttcag attccagacg ctgtatttca
     4801 cggggaaggt gtgccggtaa cggacgacga tggcgcgact gtcgcaactg gcggggaacc
     4861 tgtggatcgt cttcccgccc actgtaacga tcgctgtcct gttcgcgttg tttcagcagg
     4921 gcctcagcct gttcaacttc acgacgcagt ttttcagaac gggtaccgaa cagcatccgg
     4981 cgcagttttt ctatctgagc ccgcagatgt tctatttccc gttcatcttc ttcgatcttt
     5041 tcttcggcac gtgccagtgc aggaaggcct ccgtctcttc aaccagactc agttgctggt
     5101 ctttctgacg gagctggctt tccagttctg caatgcgaat gaggtatttc tgactcatgg
     5161 ccgtttttat aatgcggcca ggtgtttttt acaacattgt cagggcgtta aggcgggatg
     5221 tttttggctg acgccagtcc agcttatcga ggagc
//
LOCUS       DAZFWP020000243         5181 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFWP020000243 DAZFWP020000000
VERSION     DAZFWP020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.245"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            144..662
                     /locus_tag="ACHK0Z_RS23845"
                     /old_locus_tag="pgaptmp_004769"
     CDS             144..662
                     /locus_tag="ACHK0Z_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4504635.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            872..3013
                     /locus_tag="ACHK0Z_RS23850"
                     /old_locus_tag="pgaptmp_004770"
     CDS             872..3013
                     /locus_tag="ACHK0Z_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4504636.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3089..>5181
                     /locus_tag="ACHK0Z_RS23855"
                     /old_locus_tag="pgaptmp_004771"
     CDS             3089..>5181
                     /locus_tag="ACHK0Z_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4504637.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPI
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGP
                     RSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLT
                     AETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKR
                     STIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTRKLT"
BASE COUNT         1192 a         1359 c         1652 g          978 t
ORIGIN      
        1 acaaccgtaa attctagctc ggtttgtatg taaaaaatac tgaaaaatga tgcggttccg
       61 tgaagtgagc tgcatctgcc tgtctgggat taacggcagg tcattgtaag tagtaattaa
      121 ccacgttaac aaggagttta caaatgccaa caccgtgtta tatctccatc actggtcaga
      181 cccagggaaa tattactgca ggtgcattta ccgctgattc tgtcggcaac atctatgtgc
      241 aggggcacga agacgagatg ctggtgcagg aattcctgca taacgttact gtcccgaccg
      301 atcctcagtc cggtcagcct gcgggtcagc gtgctcacaa gcctttcatt tttaccgtgg
      361 cgctgaacaa agcggttccg ctgatgtaca acgcactggc ctccggcgaa atgctgccaa
      421 ccactgaact tcactggtgg cgtacctctg tggaaggcaa acaggagcat tatttcacta
      481 cccgcctgac tgattcgacg attgtcgata tgaaactgca tatgccgcat tgccaggacc
      541 cggcgaaacg cgagttcacc cagttgctcg aagtttcact ggcctaccgc aaaattgaat
      601 gggaacacgt caaatctggc acctccggtg ccgacgactg gcgcgcaccg ctggaagcat
      661 aagttaagcc aacagcatcc tgctatcagg ccggatgctg tttttgtatt acgccgtaga
      721 aagaaaggtt gtcagtaagg ggttacgttc acgggagatg gcggctgccg gaaacttcgg
      781 cggtagcgtg cctgaacgca agcgtaacac tgtactgtca cagccgcttt aacagcgaac
      841 tgtctgtttt tccttaaccc cggaggttgc tatgtcaacc ggattacgtt tcacgctgga
      901 agtggacggc ctgccaccgg atgcttttgc ggtggtctcc tttcatctga accagtcact
      961 ctcttcgctt ttttccctcg atctctctct ggtcagccag cagtttctct cccttgaatt
     1021 ccagcagatc ctcgacaaaa tggcctacct gacgatatgg cagggcgatg acgtacagcg
     1081 ccgggtgaaa ggtatggtga cctggtttga actgggggag aacgacaaaa accagatgct
     1141 gtacagcatg aaggtgtgcc caccgctgtg gcgcacaggg ctgcgccaga acttccgtat
     1201 cttccagaat gaggacatcg aaagcatcct cggcacgata ttgcaggaaa acggggtgac
     1261 cgagtggagc ccgctgttca gcgagccaca tccttcccgt gagttttgtg tccagtacgg
     1321 tgagactgat tacgatttcc tgtgccggat ggcggcggag gaaggcatct tcttttatga
     1381 ggagcacgcg caaaaaagta ccgaccagag cctggtcctg tgcgataccg tgcgttatct
     1441 gccggagtcc tttgagatcc cctggaatcc gaacacccgt accgaggtga gcaccctctg
     1501 catcagccag tttcgctaca gcgcacaaat ccgcccttct tccgtggtga ccaaagacta
     1561 cacctttaaa cgacccggct gggcagggcg ttttgatcag gaaggccagt accaggatta
     1621 ccagcgcaca cagtatgaag tgtatgacta ccccggacgt ttcaagggtg cccacgggca
     1681 gaactttgcc cgctggcaga tggatggctg gcgcaacaac gcagaagtgg cgcgcggaac
     1741 aagccgttcg ccggagatat ggccgggacg gcgaattgtg ctgacggggc atccgcaggc
     1801 gaacctgaac cgggaatggc aggtggtggc gagcgatctt cacggcgaac agccgcaggc
     1861 ggtaccggga cgcaggggtt caggcaccac gctggataac cactttgcgg taatcccggc
     1921 agaccggaca tggcggccac agccgttgct gaaaccgctg gtggacggcc cgcagagcgc
     1981 cgtcgtgacg ggaccggcag gcgaggaaat cttctgtgat gaacatggcc gcgtgcgggt
     2041 gaaatttaac tgggaccgct ataacccgtc aaaccaggaa agttcatgct ggatccgtgt
     2101 ggcacaggcg tgggcaggca ccggctttgg taacctggcg ataccgcgtg tgggtcagga
     2161 ggtcattgtg gacttcctca acggcgatcc ggaccagccg atcattatgg ggcgtaccta
     2221 ccaccaggaa aaccgcaccc ccggcagcct gccggggacg aagacgcaga tgacgatccg
     2281 ctcgaaaacc tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa
     2341 agaacaggtc tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac
     2401 cactgatgtg ataaacaacc atgccgaaaa aataggtaac aaccaggcga tcaccgttac
     2461 caataaccag atccagaaca ttggcgttaa tcagatacag acggttggtg tcaaccaggt
     2521 ggaaacggtg ggcagtaacc agattatcaa agtgggatca aaccaggttg aaaaggtggg
     2581 gatcattcgt gcgctgacgg tgggtgtggc ttaccagacg acggtaggcg gcattatgaa
     2641 tacctcggtg gcgttgttgc agtcctcaca ggtagggctg cataaatcac tgatggtggg
     2701 aatgggctac agcgtcaatg tggggaataa cgtcaccttc tcggtgggca agacaatgaa
     2761 ggaaaacacc ggacaaacag cagtttattc tgccggtgag catcttgaac tctgctgtgg
     2821 taaggcaagg ctggtgctga cgaaggacgg aagcatattt ctcaatggta cgcacattca
     2881 tctggaaggg gagtcggatg tgaacggtga tgcgccagtg attaactgga actgtggtgc
     2941 cacacaacct gtaccggatg cgcctgtgcc gaaagattta ccccccggaa tgccggatat
     3001 gcggcaattt tgagtcaggt gcaaaaaatt aaaacggaac tggtgcagtg accagataaa
     3061 ccatattaaa agcagaagga attgattcat gggcggaaaa ccggcggcgc ggcagggtga
     3121 tatgacccgc aagggactgg atattgtgca gggttcagcg ggggtgctga taggtgcgcc
     3181 gacgggagtg gcctgttcgg tatgtcccaa cagcccgata gaggagcaga agggtaaccc
     3241 ggtgaacccg ctgctgggag caaaggtgct gccgggcgag acggaccttg cgctgcccgg
     3301 cccgctgccg tttattcttt cccgcgccta cagcagctac cggaccagaa cacccgcgcc
     3361 ggtgggggtg tttggtcccg gctggaaagc gcccttcgat atccgcttac agatacgcga
     3421 tgaaggcctg atactcaacg acagcggcgg caggagcatt cactttgagc cgctgtttcc
     3481 cggcgagata agctacagcc gcagcgagtc actctggctg gcgcggggcg gagtagcggc
     3541 gcagcacagt tcgcagccgc taagcgcgct ctggcaggtg ctgccggaag atgttcgcct
     3601 gagtccgcat gtgtacctgg cgacaaacag ccttcagggg ccgtggtgga tactcagctg
     3661 gccggagcgg gtgccggggg cggacgaggt gctgccgccg gagccgcccg cataccgggt
     3721 gctgacgggc gtggtggacg gcttcgggcg gacgctggcc tttcaccggg cggcggaggg
     3781 tgatgtggcg ggcgcggtga cgggggtgac ggacggcgcg ggccgccgtt ttcacctggt
     3841 gctgaccaca caggcacagc gggcggaagt attccgtaaa cagcgcgcca cgtctttatc
     3901 ttcccctgcc ggtccccgtt ctgcttcctc ttctctggtt ttccccgaca cgctgcccgc
     3961 cggtacagga tacggcacgg ataacggtat ccggctggag gcggtatggc tgacacacga
     4021 cccggcatac ccggatgaac agccgactgc gccgctggcg cgctacacgt acacggccgg
     4081 tggtgaactg cgggcggtgt atgaccgcag cggaacgcag gtgcgcgggt ttacttatga
     4141 tgcggagcac gccgggcgga tggtggcgca ccattatgcg gggaggccgg agagctgcta
     4201 ccggtatgat gataccggtc gggtgacgga gcaggtcaac ccggaggggc tggactaccg
     4261 ctttgagtac ggagagagcc gggtaatcat cacggacagc ctgaaccggc gggaggtgct
     4321 gtacacggaa ggcgagggtg gcctgaagcg ggtggtgaag aaggaacatg cggacgggag
     4381 cattacccgc agcgagtatg atgaggcggg gaggctgaag gcacagacgg atgcggcggg
     4441 gcggcggacg gagtacagcc tgcatatggc gtcgggtgcg gtgacggcgg tgacggggcc
     4501 ggacggcagg acggtgcggt atggctataa cagccagcgg caggtgacgt cagtgacgta
     4561 cccggacggg cttcgcagca gccgggagta tgatgagagg ggaaggctga cggcggagac
     4621 ctcgcgcagc ggtgagacaa cgcggtacag ctatgatgac ccggcgagtg agctgccgac
     4681 agggatacag gacgcgacgg gcagtacaaa acagatggca tggagccgtt acggtcaact
     4741 gctggccttc acggactgct cggggtacac gacgcggtat gagtacgacc ggtacggtca
     4801 acaaatcgcc gttcaccggg aggaaggcat cagcacttac agcagttata acccgcgtgg
     4861 gcaactggtc agtcagaagg atgcgcaggg gcgtgaaatc cgttatgagt acagcgccgc
     4921 aggcgacctg acagcaacgg tatcaccgga cggtaaacgc agcaccatcg aatatgataa
     4981 acgcgggcgt ccggtgagtg tgacggaagg cggtctgacg cgcagcatgg ggtatgacgc
     5041 tgccgggcgc atcactgtgc tgaccaacga gaacggcagc cagtccacgt tccggtatga
     5101 cccggtcgac aggctgactg aacagcgcgg ttttgacggc cggacgcaac gttaccacta
     5161 tgacctgacc agaaaactca c
//
LOCUS       DAZFWP020000244         5108 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFWP020000244 DAZFWP020000000
VERSION     DAZFWP020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.246"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(437..1069)
                     /gene="yfdX"
                     /locus_tag="ACHK0Z_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             complement(437..1069)
                     /gene="yfdX"
                     /locus_tag="ACHK0Z_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU4504638.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1365..1640
                     /gene="ypdI"
                     /locus_tag="ACHK0Z_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             1365..1640
                     /gene="ypdI"
                     /locus_tag="ACHK0Z_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGU4504639.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1717..1959)
                     /gene="yfdY"
                     /locus_tag="ACHK0Z_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             complement(1717..1959)
                     /gene="yfdY"
                     /locus_tag="ACHK0Z_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU4504640.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2312..3232
                     /gene="lpxP"
                     /locus_tag="ACHK0Z_RS23875"
                     /old_locus_tag="pgaptmp_004775"
     CDS             2312..3232
                     /gene="lpxP"
                     /locus_tag="ACHK0Z_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU4504641.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3588..3659
                     /gene="ypdK"
                     /locus_tag="ACHK0Z_RS23880"
                     /old_locus_tag="pgaptmp_004776"
     CDS             3588..3659
                     /gene="ypdK"
                     /locus_tag="ACHK0Z_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU4504642.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3724..4962)
                     /gene="alaC"
                     /locus_tag="ACHK0Z_RS23885"
                     /old_locus_tag="pgaptmp_004777"
     CDS             complement(3724..4962)
                     /gene="alaC"
                     /locus_tag="ACHK0Z_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU4504643.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DDWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         1341 a         1225 c         1155 g         1387 t
ORIGIN      
        1 gcaaaatatc gtcgtagctg cactcgatat ttttaaaatc ctgtatcaag tgctgaaaat
       61 catttcatcg ccacttccaa ttcctttctg catggtaagt agtgaggacg aaaagaagaa
      121 tatgataaat attgattcgt ggtaatcaga gagagtcgcg acatttgtga tgttattctt
      181 acaactgtaa gaagcgccgg tgatttacaa gtaagggtat gattacgctt tttaaagcgt
      241 accataaaac ataacataat aaatgatttg tgatgacacg ggattattgg gatttacttt
      301 gaggaagata aaatgcgtgt ttatagaatc gcgagcgcca aataatttta tgaccacaac
      361 ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga gttaaatcct ttacctcaat
      421 atactgcctg tcagcattag ttcacaaaca gtgcttcgct gtcgacgatg attccatctt
      481 ccgcaccttt aagcgccagg tttgcctcgt agtattgctt tttatcaagc agtttctgcg
      541 catcagcaag tgcattacga gtctgtttaa gcggcatcag atattggttt tccattacgc
      601 caacaccggc caggcgaagt tcttccattg cgcccttttt atcccctttc gccatttttt
      661 cattggcaat ttttatcgcg gcttcttttt cgggagttgc tacataactc tcagaaattc
      721 cgacagatgc attaatgaca atatattggt catcattgag attggtcttt ttacctggct
      781 tagcgaactt tgcccattcg gtactgtcat ctgacagcaa agcggaagct tcattggtca
      841 gttcttttgc cttttctgga tcgccatgaa acagagccag gcgcgctacc tgcacgtcac
      901 gcatcgcata taaaccttgt tcagagatac gctctgctgc tgccgttttc tgggtttgct
      961 gaacttgctg agtttgctgt tgtgccgcta ccggtgcatt atcagcagcc catacagttg
     1021 aagatgccag aattgctgtg accatcgtag ccataattaa acgtttcata cttacctcat
     1081 tctttaacag ttttagaaaa cgccgccatc atattgggtg agcatgaaat gagaaatcag
     1141 agagcaacgg tcattttgaa agggttatct tacagttgta gttattatcg caagaagtat
     1201 tttagtctta caattgtagg aatctgcttc ctgtcgcaga gaactattct tatgtaccac
     1261 aaagagatgt catttattat atcaatgtaa atattgatta attatatgta aatacagcca
     1321 taacatcgta ggttctcagt gaaccatttt caggataaat ttctatgaaa gtaaacttaa
     1381 tacttttcag cttattttta ttggtctcta ttatggcatg caatgttttt gcattttcca
     1441 tttctggtgg tgtaagtgag aggagctata aagagactga aaaaacatca gcgatgacga
     1501 ccacacactc tacaaaactt cagccatcac aggcgatttt gtttaagatg agagaagatg
     1561 cgccaccatt aaacctcaca gaagaaatca cgcctactta tccgacaaag gcgaattatc
     1621 ttattcatcc tgtgcgatag cgtctgtcac atggcaggtg aacaatcatg tctgatgctg
     1681 atgtgaacac ctgcccaaat gagccatcct gacccactat cgcctgccat accccaccat
     1741 aaagcgaatc atcaagccac aagccggggc aagaaacata aatatccccg tcactagttc
     1801 attaccgcca gttaaccaca tggtgaagta ataaatgagt gcagcgagga tcaccatgcc
     1861 gaagaaagaa gagaccgccg ataccacgtt cagtacctga ccgatgtagc cgcttacgca
     1921 gacaatacac agggcgagaa acatccataa ataaatcata gctcaacctc ctgccgcgct
     1981 aaaaaacctg taacttcagt cactttaata gtaagacaat ccttaacctc tggcaatttt
     2041 tgctaagcat caatgaaaac agcatgttaa atgcaagact gttgtgtacg gaaaaatatt
     2101 tactttgcac gattaataat cagtagctga aagcagtcag cgagtgataa agtacagata
     2161 ccgcattcat gctcctcgtg gttatgtcct gactagtctt tacactcttt acaggaacca
     2221 ttgtcgtaca tgatggccca accaattgag tgtaccgctg tcgacatgga atcttctgcg
     2281 atacaacaat tcgtatctac agaaggtaac tatgtttcca caatgcaaat tttcccgcga
     2341 gtttctacat cctcgctact ggctcacatg gtttgggctt ggtgtactct ggctttgggt
     2401 acagcttcct tatcctgttc tctgctttct cggcacgcgt attggcgcaa tggcgcgacc
     2461 attcctgaaa cgtcgtgaat ctatcgcccg taaaaacctg gaactttgtt tcccgcagca
     2521 ttctgcggaa gaacgcgaga agatgattgc cgaaaacttt cgttcactcg gcatggcgct
     2581 ggtagaaacc ggcatggcat ggttctggcc cgacagtcgc gtacgtaaat ggtttgatgt
     2641 tgaagggttg gataacctta aacgcgcaca aatgcaaaat cgcggcgtaa tggttgtcgg
     2701 cgtccatttt atgtcgctgg aactgggcgg ccgcgtgatg ggactgtgcc aaccaatgat
     2761 ggctacctat cgtccacata ataatcagct gatggaatgg gtgcagaccc gtgggcgcat
     2821 gcgctctaac aaagcgatga tcggcagaaa taatctgcgc ggcattgtcg gtgcactgaa
     2881 gaaaggtgaa gcggtatggt ttgctcccga tcaggattat ggtcgtaaag gcagctcctt
     2941 cgcgccgttc tttgcggtgg aaaatgtcgc cacaaccaat ggcacctatg ttctctcccg
     3001 tctctctggc gcagccatgt tgaccgtaac gatggtaaga aaagcggatt acagcggata
     3061 tcgtttgttc atcaccccag agatggaagg ctacccgaca gatgaaaatc aagccgctgc
     3121 ctatatgaac aagattatcg agaaagagat catgcgcgca ccggagcagt acctctggat
     3181 ccaccgtcgc tttaaaacgc gcccggtggg agaatcgtcg ttgtacattt aacaaactgc
     3241 tcttactctt tccctcctca aatcggccat aataacctta tggtttttat ggcttttttc
     3301 ttaaggtttt ttccttcgct gaatatctaa ttattaccgt cagaaattaa actgtcgccg
     3361 atatgagaca cttgtaagtt actaaaagta aataagaaaa tgtctcttgt ctcgtttcag
     3421 ttttaggcgt acattagcgc cgtctcgagc agtttagctc agaattctgg ggtgatgccc
     3481 acggtaaaat acagggcttt taaggtcgct ttgcccggtg tcaactcact gtatcaggtg
     3541 taatgaagtc attcaggcgt aacagtaatt acgcggagag atgtaaagtg aaatatttct
     3601 ttatgggcat ttcttttatg gtcatcgttt gggccggtac ttttgccctg atgatctaaa
     3661 agcaaactgt gcaatgaaaa acaggagcca tcaggctcct gtttgcattt ctgacgacgg
     3721 tgtttattcc gcgttttcgt gaatatgttt gctgctggcg ggtagtaaac cgtcggcacg
     3781 gaacatcgct ttaatcccac gaatcgcctg acgaatacgg tcgcggtttt cgatcagtgc
     3841 aaagcgaaca tgcgtatcac catagtcgcc aaagccaatc cccggcgaga cacaaacctt
     3901 cgcttcgtta aggagcttct tggcaaattc cagcgatccc atggccgcat atggttccgg
     3961 gattttcgcc cagacataca tcgaagcctt cggcatttca accatccagc ccgcttcatg
     4021 cagcccttta accaatacat cacggcggcg tttgtactgt tcggcaatgt cgcgcacgca
     4081 ctgttgatcg ccctccagcg ccgcaatcgc cgccacctgc aacggcgtaa aggtgccgta
     4141 atcgtgatag cttttaatac gagccagggc gctgaccagc gttttgttgc caaccataaa
     4201 gccgatacgc cagcccgcca tgttgtagct tttcgacagc gtaaagaact cgactgccac
     4261 atcgcgtgca cccggtacct gcatgattga cggcgctttc cagtcatcgt agacgatatc
     4321 ggcataggcc aggtcgtgga ccaccagcac atcgtagcgt ttcgccagcg ccacaacctt
     4381 ttcgaagaac tccagctcca cgcattgcgc ggttggattc gacgggaagc cgaggatcat
     4441 catcttcggt ttcggataac tttcacgaat ggcgcgctcc agttcgttga agaaatcgac
     4501 accttccacc agcggcactg agcgtacctg cgccccggca atcaccgcgc cataaatatg
     4561 gatcgggtaa cttggattcg gcaccagcac cgtgtcacca tgatccagcg tcgccagcat
     4621 cagatgcgcc aggccctctt tcgaaccaat agtgacgatg gcttctgatt ccgggtcgat
     4681 ttcaacgtcg tagcgatcct gataccagcg ggaaatggcg cgacgtaacc gcggaatgcc
     4741 gcgtgaagtg gagtaaccat gcgtgtccgg gcgctgggcc acagtacata atttttcgac
     4801 gatatgcggc ggagtcgcac cgtccgggtt acccatgctg aaatcgataa tatcttcgcc
     4861 gcgccgacgc gcagccattt tcagttcagc ggtgatgtta aaaacgtagg gcgggagacg
     4921 atcaatgcgc gtaaagcgac gttcagggcg agtgtcagcc ataatttcct cagattaacg
     4981 ttagcgcccg gaccgtccga gcgacgctgc cacaatggtg gctgttttga aaatagcctg
     5041 attaatttct gtctgtccag cgttttagaa catttttatc aatggcttta aatgaggaac
     5101 tccacttc
//
LOCUS       DAZFWP020000245         5088 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFWP020000245 DAZFWP020000000
VERSION     DAZFWP020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.247"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..924)
                     /locus_tag="ACHK0Z_RS23890"
                     /old_locus_tag="pgaptmp_004778"
     CDS             complement(<1..924)
                     /locus_tag="ACHK0Z_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4504644.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPA"
     gene            1153..1761
                     /locus_tag="ACHK0Z_RS23895"
                     /old_locus_tag="pgaptmp_004779"
     CDS             1153..1761
                     /locus_tag="ACHK0Z_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU4504645.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1859..3250
                     /gene="selA"
                     /locus_tag="ACHK0Z_RS23900"
                     /old_locus_tag="pgaptmp_004780"
     CDS             1859..3250
                     /gene="selA"
                     /locus_tag="ACHK0Z_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU4504646.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3247..>5088
                     /gene="selB"
                     /locus_tag="ACHK0Z_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             3247..>5088
                     /gene="selB"
                     /locus_tag="ACHK0Z_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU4504647.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1099 a         1360 c         1515 g         1114 t
ORIGIN      
        1 ggcaggtaaa ttctccgggt attccgggtc gtgcgtcagc cacacggcag acagacggat
       61 gccgttgtcc cggccatatt cggtgtaacc cggcagggta tcaggaaaag cggacggttc
      121 cgtcccgccg gaaatggcct gctgccgggc ttcttctgcc cgctgcgcct gcgtggtcag
      181 taccagccgg aagtgacgcc cggcaccatc cgtcacgccg gtgatttcgc cgctgaattc
      241 accggcggct tcgcggtgga acgtctgtgt gcgcccgaag cggtccacca gcccggtcag
      301 tacccggtac ggcggcagcg gcgcaggcag cacctcatcc gcttccggca cccgctcaca
      361 ccagccgagc agccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact
      421 taagcggagt tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag
      481 tttcgccacg ccgccgcgca ccagccacag tgactcgctg cggctgtaac cgtcctcacc
      541 gggaaacagg tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt
      601 atcgcgcagc tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac
      661 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
      721 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca gaggattgac
      781 cggatggccg gacgtcactc cgccggggca caccgaacag gccacgccgg tgggggcacc
      841 aatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      901 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      961 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
     1021 acaattagga aaatatttat atcaccatga taaatatgtg atttatttgg aagtaacaaa
     1081 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
     1141 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
     1201 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
     1261 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
     1321 ggcgaatgtt ggtttgattc gccgatcatc gctgaatata ttgaattaat gaatgtcgct
     1381 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcactg
     1441 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccagcggcg
     1501 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
     1561 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
     1621 gccattgcct gtgccgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1681 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1741 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1801 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1861 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1921 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1981 tcagatgctc gacgaagcgc gagaagtgat tcgtggcagc cagacgctgc ctgcgtggtg
     2041 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaagcgcaga gcgcgctgcg
     2101 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     2161 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     2221 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgcat
     2281 tacgggggcg gaagatgcct gtatcgttaa taacaatgcg gcggcggtgt tattgatgtt
     2341 ggcggccact gccagcggga aagaggtggt ggtttctcgc ggcgaactgg tggagattgg
     2401 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     2461 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     2521 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     2581 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     2641 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2701 gattgcggcg ggcgtcagtc tggtgagttt ctccggcgac aagttgttag gcgggccgca
     2761 ggcaggaatt attgttggta aaaaagagat gatcgcccgc ctgcaaagcc acccgctgaa
     2821 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2881 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2941 agaggtcatt caaatccagg cacaacgttt acaggccccc cttgccgcac attacggcgc
     3001 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     3061 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     3121 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg gtgattggtc gtatttatga
     3181 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     3241 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     3301 gcgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     3361 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctggttt tatcgacgta
     3421 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     3481 ctgttggtgg tggcgtgcga tgacggcgtg atggcacaga cccgtgagca tctggcgatt
     3541 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     3601 gaagcgcgtg ttaatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3661 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3721 gagcatctgc ttcagttgcc ggaacgcgag cacgccagcc aacatagttt ccgcctcgct
     3781 attgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3841 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3901 cgtgcgctgc atgcgcaaaa ccagccaaca gaaaccgcca atgccgggca gcgtatcgcg
     3961 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     4021 gatgtgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacaccgctg
     4081 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     4141 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     4201 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     4261 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     4321 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     4381 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     4441 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgctcgc
     4501 tggcaacgga aaattctcga tacattagcg acttatcatg agcaacatcg cgatgaacct
     4561 gggcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctagta
     4621 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4681 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4741 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4801 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4861 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4921 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4981 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     5041 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaa
//
LOCUS       DAZFWP020000246         4985 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFWP020000246 DAZFWP020000000
VERSION     DAZFWP020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.248"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..475
                     /locus_tag="ACHK0Z_RS23910"
                     /old_locus_tag="pgaptmp_004782"
     CDS             <1..475
                     /locus_tag="ACHK0Z_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HGU4504648.1"
                     /translation="VDEFIALANSVSNRRKSRAGKSLELHLEKLFIEHGLRHFSTQAV
                     TEGNKKPDFLFPSAEAYHNVEFPVENLRMLAVKTTCKDRWRQILNEADKIHQVHLFTL
                     QEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAFITELIELYAELS"
     gene            complement(510..1943)
                     /locus_tag="ACHK0Z_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             complement(510..1943)
                     /locus_tag="ACHK0Z_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGU4504649.1"
                     /translation="MSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAE
                     LLRKVGKNEWSRETINRWINGKCLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLF
                     AGIGGIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFNLDIREVTLSDKTD
                     VLETDAYAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDV
                     ARIIRAKKPAIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIID
                     GKHFLPQHRERIVLVGFRRDLNIHKGFTLRDISRFYPEHRPSFGELLEPVVDSKYILT
                     PKLWEYLYNYAKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMA
                     TGEADFMNESNQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVP
                     VFEAVARLLEPYILKAVSADAGKTGQP"
     gene            2350..3123
                     /locus_tag="ACHK0Z_RS23920"
                     /old_locus_tag="pgaptmp_004784"
     CDS             2350..3123
                     /locus_tag="ACHK0Z_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504650.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETIIFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEVILATVRGIVRGVFIADKWLKSTRENFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(3181..3735)
                     /locus_tag="ACHK0Z_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             complement(3181..3735)
                     /locus_tag="ACHK0Z_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4504651.1"
                     /translation="MLIGYVRVSTNDQNTDLQHNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSEGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGISTLYKKFSVGDK"
     gene            3798..4259
                     /locus_tag="ACHK0Z_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /pseudo
     CDS             3798..4259
                     /locus_tag="ACHK0Z_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            4259..4852
                     /locus_tag="ACHK0Z_RS23935"
                     /old_locus_tag="pgaptmp_004787"
     CDS             4259..4852
                     /locus_tag="ACHK0Z_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4504652.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGKVAVFTGKGWSHEEDHRNETVYSTENGAAVTVDYIGAIKDGYVT
                     LSPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTVMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTVTDTSTAPDIIWPELPEA"
     gene            complement(4824..>4985)
                     /locus_tag="ACHK0Z_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             complement(4824..>4985)
                     /locus_tag="ACHK0Z_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4504653.1"
                     /translation="SRQWPGKAAIGRLKGEELAQYNLWLDYLDALELVDTSSAPDIEW
                     PTPPAVQAR"
BASE COUNT         1388 a         1156 c         1080 g         1361 t
ORIGIN      
        1 tgtagatgag tttattgcat tagccaactc tgtcagtaat cgacgtaaat caagggcagg
       61 caaatcactt gaacttcacc tagagaagct ttttatcgag cacggactac ggcatttctc
      121 cactcaggca gttactgaag gtaataaaaa accagatttc ctgtttcctt cagcagaggc
      181 atatcataac gttgaatttc ctgtagaaaa cttacgtatg ctggcagtaa agaccacctg
      241 caaagatcgc tggcgtcaga tactgaatga agcagataaa atccatcagg tacatttatt
      301 tacactgcaa gaaggtgttt cttcagcaca ataccgagaa atgaaagatg cgggtgtcag
      361 actcgttgta ccatcaactt tacataaaaa atacccagaa gcagttagag aagaattaat
      421 aacgctcgga gcattcatta ctgagctgat agagctttac gctgaactat cataggctga
      481 ctcccggctt aaaaggccgg gagatgttct caaggctgcc cagtcttacc agcatcagca
      541 gaaacagctt tgaggatata gggttccaga agtctggcaa cagcttcaaa tactggcacc
      601 acaactgagt taccgaactg ccgatatgac tgagtatctg aaaccggaat acggaatggt
      661 ttccctccag gtttttcaaa tcccataagg cgtgcgcact ctcggggagt cagcctgcgt
      721 ggtcgacgag cctgattact ctcattcata aagtcagcct ctcccgttgc catatcccaa
      781 ccacgatcaa taagaatttc tgatccgtct ttgtgatatc tggcagaaag tgtgcgtgca
      841 atgctttcct tattctcagg attgaccaac ccaaagccaa aaccattccc cttggctgca
      901 tgctttttgg cgtagttata aaggtactcc catagttttg gcgtaagtat atatttactg
      961 tcgactacag gctccagcaa ttcaccaaat gatgggcggt gttccggata aaaacgactg
     1021 atatcacgca aggtaaaccc cttgtgaata ttcagatctc gtctaaatcc gaccagaaca
     1081 atgcgttcac gatgctgagg caaaaaatgc ttcccatcga taatctttgg atcgtttttt
     1141 cccatttctg cagcatcggc aacttcgtag cccagctcgt caagggtctc cataatgact
     1201 ttgaaagttt tacccttatc atggctcttc agatttttga cattttccag cacaaaaatt
     1261 gccggttttt ttgctcgtat aatacgtgcc acgtcaaaaa aaagtgttcc ttgtgcttca
     1321 cattcaaaac catgcgcacg accaagtgag tttttcttac taacaccagc aaggctaaat
     1381 ggctggcacg ggaaccccgc aagaagcaca tcatgatccg gcacatgctc atcaatatat
     1441 gcataagcat ccgtttccaa tacatcggtt ttatcactca gcgtgacttc ccgaatatcg
     1501 agattgaatt tatgcacctg ttcatcgtta aaccagttgg ccttgtatgt acgcacagca
     1561 tctttattcc attcactggt aaaaacacac tggcctccaa tggcctcaaa acctttccgt
     1621 atccctccaa ttccagcaaa taagtcaatg aagcggaaag catattccgg atggtttgca
     1681 ggtggttctg gtagcatctt acgcagaaga gactcttcta ctgaagtcaa cgattttggt
     1741 aaacacttgc cattaatcca gcggttaatg gtttcacggc tccactcatt ttttccgact
     1801 tttctcagta attcagccac atacttctga tcatagattt ccagcacttt ctcgataagc
     1861 tttttatcat tttcctgtcg cagcttttct tccgcctcgg cttccttcag cagatgctgt
     1921 gccaacactt caaattcaga cataattcct ccaaggggtc taatgggtga aactttatca
     1981 ctcattcaac ccagaaggaa atgttttatc tggatattta aacagtgact acaacgtaat
     2041 ctagcactgg tgatgctttg ttaggcatag agaatcattc tatatacgac taatgacaga
     2101 aaaacagcag acaagtagtt tgttcataaa ttaacgcata ctatgtgtct acggttttcg
     2161 agaccggtcc aatcatcaaa cgaaacataa aattagctca cattatgagg aaaagtatct
     2221 tttttgtact atgtaaattc aaaggcttag cctcatttct ccgatggttt tctcaacact
     2281 actggttgtg agcccttgca atgatcatta atatacgtct cacaaataat tcttcataga
     2341 tattgcaaaa tggatattac tgagtttcct tctggagtaa ttgaacacct tggctggtat
     2401 gtataccgat tgattgatcc gagggacgga agcaccttct atgtagggaa aggcaaaggt
     2461 aaccgcgtat ttgcccatat gcgcggtgaa gtggcagcga ctgatgatga cgagttactg
     2521 agcaacaagc taaagcaaat tagagaaata aggttagcag gacttgaagt tatccatgtc
     2581 atccatcgac acggaatgac tgatgaaaag acggcgtacg aagttgaagc tgcacttatt
     2641 gatgcctacc ctgggttaac gaatatcatg aatggtgctg gcagcaatga attcggcgcc
     2701 gcgcatgtca aagagttgat agcaacatat caacccgaaa ccataatatt tcatcataaa
     2761 gcattaatga tttccgttaa cagaagtgca aaggattcag agctttatga tgcggttcga
     2821 tttagctggc gcattaatgt ctctcgcgcc agccaagcag aagtcattct tgctactgta
     2881 agggggatcg ttcgaggggt tttcattgct gataaatggc tcaaatcaac acgtgaaaat
     2941 ttccctacga tgaaatactg ggacgaggat cctgactttg aggcaacaca aagttctcgc
     3001 tatggttttg aaggtcgaga agccccacct gaaatagcaa atctttatct tggaaaaaaa
     3061 ataccagatg aattaagaaa aaaaggagct atgtccccgg tccgttactc acctaatttt
     3121 tgagtcttta agtgataagc ataaaccgca gcacgatctt gcatacgacg tgctacggtt
     3181 tcatttatct ccgaccgaaa acttcttata cagtgtcgat ataccaacat catagatgat
     3241 cgccaccttc tggcgaggaa cgcctgatgc aattaatcgc ccagcctgcg cccattgttc
     3301 tggtgtaagt ttgggacgac gtccaccaat tcgtccctgt gcgcgagcag cttccagtcc
     3361 agcttttgtt cgttcaacaa tcagttctcg ttccatttca gccagggcac ccatcacatg
     3421 aaagaaaaaa cgccccatcg gtgtgctggt atcaatagca tccgtcaggc tgcgaaaatt
     3481 aacgccacgt tcgcgcaact cctcaaccag aatgaccaga tgccgcatac tacgccccag
     3541 ccgatccagc ttccagacaa ccagagtgtc accttccgat aatgtcctga gcagtttttt
     3601 cagtcccggc ctttcagact ttgtaccgct tatcttgtct tcaaaaatca gctcgcatcc
     3661 tgcacagttc agcgcattat gctgtagatc tgtgttctgg tcatttgttg acacacgtac
     3721 atagccaata agcatggtag atctccctga caaaagcagg aatgatgcca tttgctcgtt
     3781 atttctgcat tttcataaac gttggtttgg gagaaggttc tccagctatt ggcgttccgt
     3841 tcttctggcc gtccgctgca atgccaaata ctgtaatcga cagctggtcc agtatggtgt
     3901 ttttgaagtt caacggcgcg aaattctctg ccactgatta tcctgtgctg gcgaaagtgt
     3961 ttccttcgct ggtattacct gaagcccgcg gtgatttcat tcgtatctgg gatgacgggc
     4021 gaggtgcaga tggtggtcgt gaattattaa gctggcaggc agctacaaac ttttctcagt
     4081 ttgccgggaa tataggcgaa ggtgcgggac acgcaattaa ctttcatgat ggcatcgccg
     4141 gaaatcagcc aggattttca cgatttaatt tcaccagtaa ctctgtgggt gatggtgtga
     4201 attttgttgc tgtcagaccg cgaaatattg catttaactt tctggtgagg gctaaataat
     4261 gaaacctgtt tttgatgaaa atgggctggc tacagtgccg ggcgatatgc gttgttttta
     4321 ttatgatgct gaaacatctg agtatacggg ctggtctgat gaatatatta atactggcgt
     4381 aagtatgccc gcctgttcca ctggtattga ccctggcgaa aacattccgg gaaaagtggc
     4441 agtatttacg ggtaagggat ggagccatga agaagaccat cgcaatgaga ctgtttactc
     4501 aacagaaaat ggcgcagctg ttacagtgga ttatatcggt gccatcaaag acggttatgt
     4561 cacgctttca ccgttaacgc catacgataa atgggatggt gagaaatggg tgacggatac
     4621 cgaggcacag catagcgccg cagtagacgc ggcagaagca caacgtcagt cactgattga
     4681 tactgtaatg gcttccatta gtctgattca actgaaattg caggccggac ggaaactgac
     4741 gcaggcagaa acaacccgac ttaacgccgt gctggattac attgacgcgg tgacggtaac
     4801 agataccagc accgcgccgg atatcatctg gcctgaactg ccggaggcgt aggccattca
     4861 atatctggcg cactggaagt atcgaccagc tccagtgcgt ccagataatc cagccacaaa
     4921 ttatattgcg ccagttcctc acctttcaga cgaccaatag ccgctttacc aggccattgc
     4981 ctgct
//
LOCUS       DAZFWP020000247         4922 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFWP020000247 DAZFWP020000000
VERSION     DAZFWP020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.249"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..573)
                     /gene="yibB"
                     /locus_tag="ACHK0Z_RS23945"
                     /old_locus_tag="pgaptmp_004789"
     CDS             complement(<1..573)
                     /gene="yibB"
                     /locus_tag="ACHK0Z_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGU4504654.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDF"
     gene            877..1809
                     /gene="rfaD"
                     /locus_tag="ACHK0Z_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             877..1809
                     /gene="rfaD"
                     /locus_tag="ACHK0Z_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU4504655.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1819..2865
                     /gene="rfaF"
                     /locus_tag="ACHK0Z_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             1819..2865
                     /gene="rfaF"
                     /locus_tag="ACHK0Z_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU4504656.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2869..3861
                     /gene="rfaC"
                     /locus_tag="ACHK0Z_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             2869..3861
                     /gene="rfaC"
                     /locus_tag="ACHK0Z_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU4504657.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3858..>4922
                     /gene="rfaL"
                     /locus_tag="ACHK0Z_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             3858..>4922
                     /gene="rfaL"
                     /locus_tag="ACHK0Z_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4504658.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKN"
BASE COUNT         1338 a         1094 c         1149 g         1341 t
ORIGIN      
        1 gaaatcaaaa acctgttgtt gagatgtgac agtaagccct ttttttatag tgaaaagatg
       61 catcttactt tcatcaaatg ggaaatccca aattttcaat cctcgagtta catttggctt
      121 acggcaatag ccaaaatcta tccacgcaac aagaggcgtt ttcactaagc ccatgttgat
      181 agctttattt acaaaatagg ccttaaggtt acataccaaa acatactctg gtgaccagta
      241 ctctgggttt tttaattgtc gaggctcaag tctatttgta aacgactcat ctttttgaat
      301 tttttcgata cgacttctga tatatctaaa tttctttttt atatcgataa cgataaccgt
      361 tgttggtttt ccgttgcgaa tcgcttcaac ccttggtttc aaatcgggag aagtgaaaat
      421 aatcatctca ttttcaagtg cggccagacg ctcaaagtaa ctaaaataaa catcaactga
      481 acgagctagt ttttcacgga accctttatt agcagtccaa tcccctctac caatgtcaaa
      541 atatgcagtg ataattgtgg tggatgattt cattactaat aagaccatta atataaaaaa
      601 agaattatat cataaatatt aacagaatgt atactgtata ttatccattc aaatgcttcc
      661 gttatttatc atacatcgga atattgatac taaagcacta ttcacatgca aaaccaacat
      721 ccgccatgaa ggactagcta aaacccaaac tagtttgttg caattagcat ccttgcacct
      781 ctatgtaaag ggctgaaggg attcggatgt gatggtatga ttacagacat tcgtgtctga
      841 gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac cggcggcgcg
      901 ggctttatcg gcagcaacat cgttaaagcc ctgaatgata aaggcatcac cgatattctg
      961 gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct gaatatcgca
     1021 gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga gttcggcgat
     1081 gtcgaagcga ttttccacga aggcgcgtgc tcttccacca ccgagtggga cggcaagtat
     1141 atgatggata acaactatca atactccaag gagctgctgc actactgcct ggagcgtgaa
     1201 atcccgttcc tgtacgcttc ttccgcagcc acctacggcg gacgcacctc cgactttatt
     1261 gaatcccgcg agtacgaaaa accgttgaac gtctacggtt actcaaaatt cctgtttgat
     1321 gaatatgttc gccaaatcct gccggaagcg aactcgcaga ttgttggctt ccgctatttc
     1381 aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc tttccatctc
     1441 aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga gaactttaaa
     1501 cgcgacttcg tctatgttgg cgatgtggca gatgtaaacc tgtggttcct ggaaaatggc
     1561 gtttccggca tcttcaatct cggcaccggt cgtgcggaat ccttccaggc tgtagctgat
     1621 gctacgctgg cttatcacaa gaaaggccag atcgaataca ttccgttccc ggataagctg
     1681 aaaggccgct accaggcgtt cactcaggca gatctgacaa atctgcgcgc ggcgggttac
     1741 gacaaaccgt tcaaaaccgt tgctgaaggt gtaacggaat acatggcctg gctgaatcgc
     1801 gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt tggcgacatg
     1861 atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc gataatcgat
     1921 gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt taacgaagct
     1981 atccctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa actgggtcat
     2041 agcctgcgtg aaaagcgcta cgaccgcgcc tacgtcttac ctaactcctt caaatccgca
     2101 ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga gatgcgctac
     2161 ggtttactca acgatgtacg cgtgctcgat aaagaagcct ggccgctaat ggtggaacgc
     2221 tatatagcgc tggcctatga caaaggcatt atgcgcacag cacaagatct gccgcagcca
     2281 ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg taatcaattt
     2341 tcgctttcat cagaacgtcc gatgattggt ttttgcccgg gtgcggagtt tggtccggca
     2401 aaacgctggc cacactacca ctatgcggag ctggcaaagc agctgattga tgaaggttat
     2461 caggtggttc tgtttggctc ggcgaaagat catgaagcag gcaatgagat tcttgccgct
     2521 ttaaataccg agcagcaggc atggtgtcgg aacctggcgg gggaaacaca gcttgatcaa
     2581 gcggttatcc tgattgcagc ctgtaaagcc attgtcacta acgattctgg cctgatgcac
     2641 gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccaagtag cccggacttc
     2701 acaccgccgc tatcccataa agcgcgcgtg atccgtctga ttaccggcta tcacaaagtg
     2761 cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac tccccagcgc
     2821 gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat gcgggttttg
     2881 atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact cactgatgcc
     2941 cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaagggtt cgcacagatt
     3001 ccttcctggc acgctgccgt tgagcgagtt attcctgtgg caatacgtcg ctggcgtaaa
     3061 gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc gctacaagca
     3121 gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc actggtgaca
     3181 cgtctggcgc atggcgtaaa gcatggattg gactggcaaa ccgctcgcga acctttagcc
     3241 agcctgtttt acaattgtaa gcatcatatt gcaaaacagc agcacgccgt agaacgcacc
     3301 cgcgaactgt ttgccaaaag tttgggctat agcaaaccgc aaacccaggg cgattatgct
     3361 atcgcacagc attttctgac gaacctgcct acagatgctg gcgaatatgc cgtatttctt
     3421 catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga attgattggt
     3481 ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca tgaggaagaa
     3541 cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa gatgagtctg
     3601 gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga tacggggtta
     3661 agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc aaccgatccg
     3721 ggattaattg gtgggtatgg gaagaatcaa gtagagtgtc gttctacaag tatgtctctt
     3781 gcagatttgc cagctcaaac cgtttttcaa aatttaaatc ttgaaataat aaccaataag
     3841 ttgacatcgg agataagatg acctcaacat tatttttctc tctcgagaaa aaaaactgga
     3901 tagcgtactg gaacagagct ctcgtattct tattcattac cacctatttt ttgggtggga
     3961 ttacaaggta taaacatctt attgttattc ttatgacaat aacgacaatc gtctatctct
     4021 gcaaacggcc aaaacactat ctctcactat ttaaaacatt tctttttggt agtgttgcca
     4081 tattaactat tgctgcattg ctgtcacttc ttcaatcccc tgatgcaggt gctagcatga
     4141 aggaaatttt caaagctatt attgagaata ctttactatg cacaatagca ataccgatca
     4201 tattgagaga cgagaaaaga gaagatgtcg aaaaaatcgt tttcttctca tttattagtg
     4261 cgttgggctt acgctgtttt tctgaattga ttacctatta taaggactat caacaaggga
     4321 taatgccatt cgcagattat agacaccgta gcatttctga ctcgatggtc tttttattcc
     4381 ctgcattgtt aaatctctgg cttatcaaat cagcaaaata ccgcatttct tttgtggttc
     4441 taagcgttat ttttattttt ctgatattag gaactttatc cagaggggcc tggctttccg
     4501 tgttagtcat tggattaata tggattctga tgtttaaaca atggaagtta ctattagtag
     4561 gagtaatggt tgccatcatt gcattgtcgg ttattttcac acataaggag atgaccgcaa
     4621 agctaacgta taaacttcaa caaactaata gttcttatcg ctatgcaaat ggtactcaag
     4681 gcagcgcact cgatctaata ttagaaaatc ctgttattgg ttatggttac ggtaacgttg
     4741 catataaaga tgtctataat aaacgtgtca ttgattatcc agaatggacc tttagacaat
     4801 caatagggcc acataatttt gcgctattca tctggtttgg cactggttta ttagggctgg
     4861 taagtcttat gatgctatac tgtgcaatat tgaaagagtg tataaaaaat ggcgtcaaga
     4921 at
//
LOCUS       DAZFWP020000248         4902 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFWP020000248 DAZFWP020000000
VERSION     DAZFWP020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.250"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..490
                     /locus_tag="ACHK0Z_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             <1..490
                     /locus_tag="ACHK0Z_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion protein GogB"
                     /protein_id="HGU4504659.1"
                     /translation="NTLTSAVWDYKSPRKYKKDALIKRALSESDRGINFKQYLKNHSQ
                     YNVTMADLSVCNRDKLWAKTSKAGLEFQLLVRNKMVHFCADGLVDTLKNIANKGDAYG
                     QSITASELRWIYRNQDNEQIMKNIKFYLRGKEVPAEKILGMPEWKDYHPKRSGLISEY
                     SK"
     gene            complement(1693..2535)
                     /locus_tag="ACHK0Z_RS23975"
                     /old_locus_tag="pgaptmp_004795"
     CDS             complement(1693..2535)
                     /locus_tag="ACHK0Z_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4504660.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSVSHILAVRKSTLLQIDTLIRQ
                     LAEISVMTESIGGKTALDWAMKQDFRCGCWLMEKTETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2620..2817)
                     /locus_tag="ACHK0Z_RS23980"
                     /old_locus_tag="pgaptmp_004796"
     CDS             complement(2620..2817)
                     /locus_tag="ACHK0Z_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4504661.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLPDQNR"
     gene            complement(2829..3317)
                     /locus_tag="ACHK0Z_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             complement(2829..3317)
                     /locus_tag="ACHK0Z_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4504662.1"
                     /translation="MNLSLTLEADSLNVQALNMGRIVVDVDGIELAELINNVTENGYS
                     VRVVEDSGQQSTCTPPPLTILAGIRCSTAHITETDNAWLYSLSHQSSNFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(3314..3691)
                     /locus_tag="ACHK0Z_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             complement(3314..3691)
                     /locus_tag="ACHK0Z_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4504663.1"
                     /translation="MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRSHYRTVNDITLGKRTEAKR"
     gene            complement(3738..4112)
                     /locus_tag="ACHK0Z_RS23995"
                     /old_locus_tag="pgaptmp_004799"
     CDS             complement(3738..4112)
                     /locus_tag="ACHK0Z_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4504664.1"
                     /translation="MSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            complement(4187..4408)
                     /locus_tag="ACHK0Z_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             complement(4187..4408)
                     /locus_tag="ACHK0Z_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4504665.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            complement(4477..>4902)
                     /gene="radC"
                     /locus_tag="ACHK0Z_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             complement(4477..>4902)
                     /gene="radC"
                     /locus_tag="ACHK0Z_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4504666.1"
                     /translation="LIHRALKTLDRHLHEPGVAFTSTRAAREWLILNMAGLEREEFRV
                     LYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAAVVLAHNHPSGEVTPGKT
                     DRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         1275 a         1087 c         1267 g         1273 t
ORIGIN      
        1 gaatacgtta acaagtgctg tatgggatta taaatctcct cggaaatata agaaagacgc
       61 tcttatcaag agagcattaa gtgagtctga tagaggaatt aactttaagc agtatttgaa
      121 gaaccatagt caatataatg tcactatggc cgatctttct gtgtgtaatc gtgacaagtt
      181 atgggcaaaa accagtaagg ctggcctgga gtttcagtta ttagtaagga acaaaatggt
      241 gcatttttgt gccgatgggc ttgtcgatac tctaaaaaat atagccaata agggggatgc
      301 ttatgggcaa agtattacag ccagtgagtt gcgatggatt tatcgtaatc aggataatga
      361 gcagataatg aaaaatataa agttttatct acgtgggaaa gaagttccgg cagagaaaat
      421 tttaggcatg ccagaatgga aagattatca tccgaaacgt tctggattga tatctgaata
      481 ctctaaataa tgcacttcta ttatgacagt gcatatcagt aaagtaatat cggcaggaga
      541 taaaattatg ccattgtcaa tcggaagttg tttttcatgt cacacaggac gtcgaccaga
      601 aatatctgat ccaataattg ataagccatc aactaaaaat tgtgaactga tatcaacaac
      661 atgtaatgtg gatggtataa cggttatatc cagaagctct tatggttttg atatgaagcc
      721 acctggtgcg ggggagcgag ctccacggct aaaattgtcg gcctcagaag ctcagtggat
      781 ggcagcaata atagatgcag aggggcataa tataagcaat acctgaccat ttactcagtg
      841 atgtgttatc tgagggctgg cggagtttgt acacagattg tgccgttgcc tgatgttgat
      901 atgttcagct cagcatcaaa tgaaggcgat tatggatgaa aaacggacct ccatggaggt
      961 ccgttgatat gagtctggtg cctgattcgg aatcgaatta aggattcatt gacacggcaa
     1021 tgaaatcacc aaataacagt aagttacctt tacacttcga ttccaaatgt accactattt
     1081 tgtactgttc aagtcgtatt acttctgata catcatttta tgatctcgtg gaggacagag
     1141 gattctggta agggtaagaa gcgagaagga aagaagagtc tgttgcaggt ggacggagtg
     1201 gtgaagagcc ccatgtcaat ggcgtatcag gttttcccgc ttgttattga agattttccc
     1261 accttattct ccgcgacaaa atcacgttaa aacctgcgga ttaaggcgaa atgataactc
     1321 tcctgtttcc ccgtgttttc tacttcatta tgaatgttct gagaaaaggg aaccgtagaa
     1381 agaccgtagt agtagagtaa ggacaaggca cacatcatag gatgttgggg tgtctaaaag
     1441 taacgaacat cggcgaaaat agtgagaaat acaacaaaca aataacgtat tgcaacgacc
     1501 agaaatctac cgtagccaga aaagcaaaaa gccccgcttt tcagcgaggc cttatgaatt
     1561 tggtgccgga ctcggaatcg aaaatttctt ttatcatatt gatattaata taatttgttt
     1621 ggtttgatta aaaatatacc ctcaattata cccgcagtcc cttttgatat cgaaagggaa
     1681 tcacactcgg gatcaggctg agagcacaga aggaaagtac ctggctatta tatcgttaat
     1741 tctgtcaatc agttccagcc ttttgaaagt gatgtgtgca gatcccttct taaagtattt
     1801 aatgatgaac atctcgtctt catagcgctc tttaccctga acggactgga tgtgaccatc
     1861 cagacgaata gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatgtag
     1921 cattctttcc aggtcgacga gtcggtcagt ctgttgcccg gtgataagat gaaaccccca
     1981 ccggtcccac ctcactaggt tgttgacgat aattttactg ccaaatttac aggggcaatt
     2041 ggttttgtaa ttccagctca gccctctgaa gaggttgatc acacctcgct caaatacttc
     2101 gtccttattc tggtgcagtt gttcaaacgt actcaggata ttcgcctcgc tgatttccgg
     2161 aaaattatcg tactccagtg atcggtacca tgtatcacgt gcctgtgcat ccattaatga
     2221 cagcatccct gaacgttgca tcaggtcacg ccagatttcg cggtcaaggt tgcgggtgat
     2281 agctttcatt gcggtttccg ttttctccat cagccagcag ccgcagcgaa aatcctgttt
     2341 catggcccag tccagtgcag ttttaccgcc aatgctttcc gtcataactg agatttcagc
     2401 cagttgccgg ataagtgtgt cgatttgcag cagtgtactc tttcgtacag ccagaatgtg
     2461 gctgactgag gttgagcaaa taacgtccgt atgcccgttg accacttccg gacgggaggc
     2521 tgtgatatca gacataatcg atattccttt ttacagataa acgaaaacgc cagccgtatt
     2581 caggctggcg tcagtggaat gaagagtggt gaatgagact caccggttct ggtctggaag
     2641 ctgcagctgg cgcagggtac ggatatcctc cctggcctgt tcaatacagg gcaacagtgc
     2701 tgccgaatcc gttttatctt catcgttgtc aaagataatt cccgattcgc agtcgatatt
     2761 gtcctgcagc catgcaatca gaatatccag cgctgtttcc gtggttaatg atttcatgtt
     2821 gtttctggtt accagtcgaa agtgggtaaa tccggcagac attcggcgct ggcatccaga
     2881 tgaatgagac tgacgccata acgtcgggta agtgtgacaa ccagacggcg gaacgttctg
     2941 gacaggccca ggcgtttaag ccgcagaacc gggtatgacc acgcatcagt acgtaacaga
     3001 tatccgctac ctgtaaaatg aatccattct gattcgccga agttactgct ctgatgcgac
     3061 agcgagtaca gccaggcgtt gtctgtttcc gtgatatgtg cggtgctgca gcgtatgccg
     3121 gcaaggattg ttaacggtgg tggtgtgcag gttgactgtt gcccggagtc ctccaccacg
     3181 cggactgaat aaccgttttc agtgacattg ttaatcagtt cagcgagctc aataccatcg
     3241 acgtcaacga caatgcgtcc catgttcagt gcctgtacgt taaggctgtc ggcttcgagc
     3301 gtcagggaaa ggttcatcgt tttgcctccg tgcgtttacc cagggtaatg tcatttaccg
     3361 ttctgtagtg gctgcgggtc atcaggccgg ttgcccggcg ggcccggaga atatcaatgc
     3421 tgttgattaa ctgcgacggg gctcctgcgc tgaatcccgg ctggtcagtt cgtaccagcg
     3481 cgtatttttc aacgagaaag ttcaccgcat cacacagtga aatccctgcc tcaatatgct
     3541 gctcaatcac acgttcatca gcgaacggtg tgtcattcag cgtgaggcca tagtgctggt
     3601 ccagcagtcg ggtgagcagt gtctgccaga tggtgacggg agacgggcag cccgatgcct
     3661 cccgtacgtg tgtgtcaggt aatgttttca tcttaaattc tctgtaactg gtagtgctaa
     3721 aggtgagata ttcggggtta tgaggtggtt gcgggtgcca gtgtcggata aacagccata
     3781 taaacgtaac cacagctgcc gagggtgtcg gcttcgcagg tcagccctct ggcatacagc
     3841 gtgacggtat gctgatggcg gggattcagt tcaccgctgg tgagcatgag ttccagttgt
     3901 ttcatcagca gcggaaaggc ctggtccaga tgatacgcat ccgcgtcgct aaactgcccc
     3961 ctgataccgg cgcggtcggc aaggtaatgt aattgattgc cctcctgcac cagacgtgcc
     4021 ccgaaacagg gcgtcacggt gcaggggagc ccccaccagg ggcggtcgtt gttgtcgttg
     4081 ggataatttg tctcatggag tgtgcctgac acgataaaat cctcacaaaa aatcagttaa
     4141 gaatggtccg gtggcagcga taattctgct catcatttct cctgactcag ttcagtgtga
     4201 cgctcatcag ccggacatac gagccaaaac tgtccttacg gcgttctgca aacacggcaa
     4261 gaacgccggg aatatcctgt acttcgcgac cggtatacgc ctcagcgcta ccgtgccagc
     4321 gatacttacc ggtacagaac ggaaaaagac gggatgcagg atgctgttgg tgaatacgca
     4381 tggcttcacc acgggtgatg attctcatga tgtgatacct ctgaagacag aagataaaag
     4441 cgaaaacagg tatgatgtgg tggtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4501 ggagaaaacc tggctgccac cgactatcag atggtccggc acccggatgt caaccagggc
     4561 cagcgcctgt accagacgtt cagtgataag ccggtcagtc ttaccaggtg tgacttcacc
     4621 ggacgggtga ttgtgtgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4681 cacttcccgg ggatggactt ccgtgcggtt gatggtgccg gtgaagaggg tttcaccggc
     4741 aatcagctga ttctggttgt tcagatacag cacccggaat tcttcacgct ccagtcctgc
     4801 catgttcaga atcagccatt cccgtgccgc acgggtggag gtgaaggcca cgccgggttc
     4861 atgaagatgg cggtccaggg ttttcagggc ccgatgaatg ag
//
LOCUS       DAZFWP020000249         4847 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFWP020000249 DAZFWP020000000
VERSION     DAZFWP020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.251"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            213..797
                     /gene="yadN"
                     /locus_tag="ACHK0Z_RS24010"
                     /old_locus_tag="pgaptmp_004802"
     CDS             213..797
                     /gene="yadN"
                     /locus_tag="ACHK0Z_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4504667.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            902..1642
                     /gene="yadV"
                     /locus_tag="ACHK0Z_RS24015"
                     /old_locus_tag="pgaptmp_004803"
     CDS             902..1642
                     /gene="yadV"
                     /locus_tag="ACHK0Z_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4504668.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1677..4277
                     /gene="htrE"
                     /locus_tag="ACHK0Z_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             1677..4277
                     /gene="htrE"
                     /locus_tag="ACHK0Z_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HGU4504669.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAEPVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4294..>4847
                     /gene="yadM"
                     /locus_tag="ACHK0Z_RS24025"
                     /old_locus_tag="pgaptmp_004805"
     CDS             4294..>4847
                     /gene="yadM"
                     /locus_tag="ACHK0Z_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4504670.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATF"
BASE COUNT         1426 a          990 c         1064 g         1367 t
ORIGIN      
        1 atttattttt gcttaatttt gaattaagaa aatttaattt gttcaaaagc ccttaaagtg
       61 cacaataggc gtcctaatga acaatggttg taaattttgg taacaaatat cattgctcat
      121 taatatctag catctgctta taacatttgt gtcatgaata gtcattcttc aaaccagtag
      181 tctgactaat ggaattttat tcaaggattt ttatgtctaa aaaattaggt tttgccctgt
      241 ctggtcttat gctggcaatg gttgcaggta ctgcttctgc tgatatggat ggtggtcagt
      301 taaatatcag tggtctggtt gttgataaca cctgtgaaac ccgcgttgac ggcggtaata
      361 aagatggtct gatcctgctg caaactgcaa ctgttggtga aatcgatgct ggcgtgctga
      421 atgacaccgt tggcgctaaa gccaaacctt tcagcatcac cgttgactgc agcaaagcaa
      481 atccgaatcc aggtagtaca gctaaaatga ccttcggttc tgttttcttt ggtaatagca
      541 aaggaacgct gaacaacgac atgtctatta acaacccgtc tgacggcgtg aatatcgctc
      601 tgcacaatat tgatggttcc actatcaaac aggttcaaat taacaaccct ggcgatgtgt
      661 atactaaagc cctggatgca acgacaaaat ctgctgttta tgattttaaa gcgtcttacg
      721 ttcgtgccgt cgcagaccaa acagcaactg ctggttatgt aaaaactaac actgcataca
      781 ccattactta tcagtaagta ttcaccaatt aaattgattg aatgaatata cagggaatag
      841 tgttaataat ttctattttt atattattcc ctgttttaat taactctgtc agggatggtt
      901 tatgtttttt aacaccaaac atacaacagc tttatgcttc gtaacctgta tggcttttag
      961 ttcatcgtct attgcggaca ttgtcatttc gggtactcgc gtaatatata aaagcgatca
     1021 aaaaagtgtc aacgtacgtc tggaaaataa agggaataac ccgttgcttg tccagagttg
     1081 gttagatact ggcgatgaca acgctgagcc tggcagtatt acagtccctt ttactgccac
     1141 gccgccagta tcgcgtattg atgccaaacg tgggcaaaca atcaaattaa tgtacacagc
     1201 cagcacctca ctgcctaaag acagagagag cgtgttctgg tttaacgtac tggaagttcc
     1261 accaaaacca gatgcagaaa aggtcgcgaa tcaaagtctg ctgcaactgg catttcgcac
     1321 acgtataaaa cttttctatc gcccggatgg attgaaggga aatccctctg aagccccgtt
     1381 agccctgaag tggttctggt caggttcaga aggtaaggcg tcattgcgag tgaccaatcc
     1441 aaccccttac tacgtctctt ttagcagtgg tgatttagaa gctagcggta aacgctatcc
     1501 gattgatgtg aaaatgattg caccatttag tgatgaggtc atgaaagtca ctggccttaa
     1561 tggcaaagca agttctgcaa aagtacattt ttacgccatt aatgattttg gtggcgccat
     1621 tgaaggtaat gccagtctgt aatcaggcaa ggatataatt ccgcaggaag cataacgtga
     1681 ctataaaacc aactaaaaat tatcataatc atctgacccg tatcgctacg ttttgcgcgc
     1741 tgctgtattg caatagtgct ttctgtgctg aacctgttga atatgaccat accttcctga
     1801 tggggcagaa tgcctcaaat attgatctca gccggtatag tgaaggtaac cctgctatac
     1861 cgggtatgta cgacgtcagt gtttatgtca acgatcaacc tattattaac caaagcatta
     1921 catttattga aattgaagga aaaaaaaacg cacaagcctg tatcacactt aagaatttat
     1981 tgcagtttca tataaattct cccgatataa ataacgaaaa agccgttctg cttgccaggg
     2041 atgaaacact cggcaattgc cttaatttga cggaaattat ccctcaggct tctgttcgtt
     2101 atgacgttaa cgagcaacgt ctggatatag acgttcctca ggcatgggta atgaaaaatt
     2161 accaaaacta tgttgatcca tcgttatggg aaaatggcat taatgcggcc atgttgtcat
     2221 acaacctcaa cggatatcat agtgaaactc ctggtcgaag aaatgacagc atttatgctg
     2281 cttttaacgg tgggatgaat ttaggtgcat ggcgactgcg tgcctcaggc aactacaact
     2341 ggatgaccga ttctggcagt aattatgatt ttaagaatcg gtatatacag cgtgatatcg
     2401 cctcgctgcg ttctcaactc attcttggtg agtcttatac aacgggcgaa acctttgatt
     2461 ctgtcagtat ccgcggcatt cgtttataca gcgacagccg catgttgcct ccgacattag
     2521 cgagttttgc gcctatcatt catggcgttg ctaataccaa cgccaaagtc actattacgc
     2581 aaggtggcta taagatttat gaaacgactg tgccgccagg cgctttcgtc attgatgatc
     2641 tgagtccgtc aggttacggc agcgatctta ttgttactgt cgaagaatcc gatggctcaa
     2701 agcggacatt ctcgcaacct ttttcatcgg tcgttcaaat gttacgccct ggcgttggac
     2761 gttgggatat tagcggcggc caggtcttaa aagatgatat tcaggatgag cctaatttat
     2821 ttcaggccag ctactactac ggtctgaata actatctaac gggttatact ggtattcaga
     2881 ttaccgataa taactatacc gctggtttgt taggtcttgg tctgaatact tcagtcggtg
     2941 cattttcttt cgatgtgact cactccaatg ttcgtatacc ggatgataaa acatatcagg
     3001 gacaaagtta tcgtgtttcc tggaacaagt tattcgaaga aacaagtact tcactgaata
     3061 tcgcggccta tcgctattcg acacagaatt accttggtct taatgatgca ctaactctaa
     3121 ttgatgaagt gaaacatccc gaacaagatc ttgaaccgaa atccatgcgt aattactcgc
     3181 gcatgaaaaa tcaggttacg gtcagtatta accaaccgct aaaatttgag aaaaaagatt
     3241 acggttcatt ttatctttcc ggaagttggt ccgattactg ggcttccgga caaaatcgta
     3301 gcaattactc tattggctac agtaacagtg catcctgggg cagctacagt gtcagtgccc
     3361 agcgttcatg gaatgaagac ggcgacactg acgatagcgt ttatctcagt ttcaccattc
     3421 ctattgaaaa attacttggc actgaacaac ggacttcagg tttccagagt attgatactc
     3481 aaatgagcag tgactttaag ggtaataacc aactcaacgt cagtagcagt ggctatagtg
     3541 ataacgctcg cgtcagttat agcgtaaata ctggctatac gatgaataaa gccagcaaag
     3601 atttgagtta tgttgggggt tatgccagct atgaatcacc gtggggaagc ctggcaggtt
     3661 cagtttctgc aaatagcgat aacagtcgtc aagtgtctct cagcaccgac ggtggttttg
     3721 tattgcatag cggcggcctg accttcagta atgacagttt tagcgactcc gatacactgg
     3781 cggtagttca ggctccaggt gctcaaggag cgcgaataaa ttatggcaac agtactatcg
     3841 atcgatgggg ttatggtgtc accagcgctc tttctcctta tcatgaaaac cgtatcgcgc
     3901 tggatatcaa cgatcttgag aacgatgttg aattaaaaag taccagtgca gtagctgtac
     3961 cgcgtcaggg ttcagtcgtc tttgctgatt ttgaaaccgt gcaagggcaa tcagccatta
     4021 tgaacatcac acgaagtgat ggtaaaaata ttccatttgc tgcagatatt tatgatgagc
     4081 aaggcaatgt cattggtaat gttggacagg gtggacaagc atttgttcgt ggtattgagc
     4141 agcagggaaa tatcagcatt aaatggctcg aagaaagtaa acccgtaagt tgtcttgcgc
     4201 attatcaaca aagttcagaa gcagaaaaaa tagcgcaatc tattattctg aatggaattc
     4261 ggtgtcagat tcagtaacta caagggacgt caaatgataa aaataacgcc acataaaata
     4321 gcgatactga tgggaatatt attatcacca tcagtatttg caacggatat taatgtagag
     4381 tttacagcca ctgtcaaagc gacaacctgt aacatcacac ttactggtaa taacgtcacg
     4441 aatgatggca ataataacta cacattgaga atccctaaga tgggtctgga taagatcgcg
     4501 aataaaacga cagaatctca ggctgatttt aaactggttg ctagtgggtg cagcagtggc
     4561 atcagttgga ttgataccac tctgaccgga aatgcatcat caagctcacc taagcttatt
     4621 ataccgcagt ctggtgattc atcttcgacg acaagtaata tcggtatggg tttcaaaaaa
     4681 cggactactg atgatgccac tttccttaaa cctaacagtg cggaaaagat acgctggagc
     4741 acagacgaga tgcagcccga taagggtctt gaaatgaccg ttgcgctacg tgaaacagat
     4801 gcagggcaag gcgtaccggg gaattttcgt gcactggcaa cgtttaa
//
LOCUS       DAZFWP020000250         4817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFWP020000250 DAZFWP020000000
VERSION     DAZFWP020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.252"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..1079
                     /gene="hemB"
                     /locus_tag="ACHK0Z_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             105..1079
                     /gene="hemB"
                     /locus_tag="ACHK0Z_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU4504671.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1185..2036)
                     /gene="tauD"
                     /locus_tag="ACHK0Z_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             complement(1185..2036)
                     /gene="tauD"
                     /locus_tag="ACHK0Z_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU4504672.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2033..2860)
                     /gene="tauC"
                     /locus_tag="ACHK0Z_RS24040"
                     /old_locus_tag="pgaptmp_004808"
     CDS             complement(2033..2860)
                     /gene="tauC"
                     /locus_tag="ACHK0Z_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU4504673.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2857..3624)
                     /gene="tauB"
                     /locus_tag="ACHK0Z_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             complement(2857..3624)
                     /gene="tauB"
                     /locus_tag="ACHK0Z_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU4504674.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3637..4599)
                     /gene="tauA"
                     /locus_tag="ACHK0Z_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             complement(3637..4599)
                     /gene="tauA"
                     /locus_tag="ACHK0Z_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4504675.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDYAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1104 a         1399 c         1238 g         1076 t
ORIGIN      
        1 ctctttcatt ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct
       61 tcaggtatac tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc
      121 aacgccctcg tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac
      181 ttagccttaa cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag
      241 ccgttgaagc catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg
      301 aacgcatcgc caacgccggt atccgttccg taatgacttt tggcatctct caccataccg
      361 atgaaaccgg cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct
      421 gcaagcagac tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt
      481 ctcacggtca ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgca actctggaaa
      541 atttaggcaa gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg
      601 ccgcgatgga cggccaggta caggcgattc gccaggcgct ggacgctgcg ggatttaaag
      661 atacggcgat tatgtcgtat tcgaccaagt tcgcctcctc cttttatggc ccgttccgtg
      721 aagctgccgg aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc
      781 gtcgcgaggc gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg
      841 ttaaacctgc tggagcatac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc
      901 cgattggcgc gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg
      961 gtgctataga tgaagagaaa gtcgtgctcg aaagcttagg ttcgattaag cgtgcgggtg
     1021 cggatctgat tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat
     1081 ttttttcctc tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc
     1141 ctaaccctct ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa
     1201 aacggtttat ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg
     1261 gcattggcat agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag
     1321 cgccaacgca cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct
     1381 tcgctctctt tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc
     1441 gcctgtttac cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc
     1501 gcgaccgcct cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg
     1561 aacgatttac ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca
     1621 gagagcgcct cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt
     1681 aactctttag ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta
     1741 tgccagttgt cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca
     1801 accccttcgg catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc
     1861 gcgcgttgct gctgcggcgt aatagtttga tcgcgcagaa acaccacctg atggcgcagc
     1921 accgcatggt aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc
     1981 gaaatttgtg cgccgatata cggccccagc ggggtaatgc tcagacgttc actcattgta
     2041 cttctccatg ccagggcgtc agacggcgct gtaacgcgcg caaacccagt tctaaaagaa
     2101 aggcgataat cgcaatcacc gcgattcccg ccagcaccac gtcagtcgcg agaaattcac
     2161 ccgctgactg aaccataaat cctaaaccgc gcgtcgcggc aatcagctcc gccgctacca
     2221 gcgtagacca gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc
     2281 cgggcaaaat gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac
     2341 gaatgcgaac ctgctgcgcg cttttcaccc ccgccagcgc cgacatcgcc accggtgcaa
     2401 aaatcgctaa atagatcagt aagatcttcg aggtttcacc aataccaaac cagatcacca
     2461 tcagcggcaa ataagccagc ggcggcacgg gacgataaag ttcgattacc ggatccagaa
     2521 tgccgcgcac cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg
     2581 ccgcgagtag cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg
     2641 cgtccataaa tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg
     2701 gcggcaaaaa cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta
     2761 aaaccgctaa cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca
     2821 gccgccgcga atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc
     2881 aaatacgcgg cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct
     2941 gcggctcgac tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac
     3001 acggccaggg ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat
     3061 atcgtgggta atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagagt
     3121 ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga gtaataacag
     3181 ctggggattc gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag
     3241 ctgccagatg tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc
     3301 gatttccagt cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc
     3361 ctgtacattg cgccacggta gtagcccttc attctgaaaa actacgccac gctctgctcc
     3421 cggtccctca atacgcttac ccgccagtag aatgctgcca tgctgataag gcacaaaacc
     3481 ggcaatcaga ttcagcaacg tggttttacc gcagccggac ggccccagca ccaccagtag
     3541 ctcgccgctt tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc
     3601 gtaaagatga gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac
     3661 gtactggctg taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc
     3721 ggtgtcgatg atcgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt
     3781 cagataggta ttccccttca ccagccccgg aacgtcacct tcaggcacgc cgcttaaacg
     3841 cgccagtttg ctgatgtttt ccggctgttt cagccacacg tctgggttag caatgtacgg
     3901 ttgctgagca tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc
     3961 ataatctttg cgcaccaccc agacgtccag cgttggcgcg ccccactgcc cgacctgttc
     4021 agaatcggtc aacaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata
     4081 agcaccatca atatctcccc gctgccaggc agcgataatc gcgggcggct gcaggttcac
     4141 aatctccact tgcccgggtt taatgcccca gtgtttcagc gccgccagca ggctgtagtg
     4201 ggtggtggag ataaacggta cggcgatgcg tttgccaatc agatcttccg gtttgctgat
     4261 agttttcttt accaccagcg cttcggagtt acccagtttt gacgccagca agaagacttc
     4321 aatcggcacc tgttggctgg ctgcaaccgc taacgggctg gaaccgaggt tgccgatttg
     4381 cacatcgcct gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc
     4441 cacgctcgcc ccgctctctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc
     4501 ggctgaggtt tgataggcca cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc
     4561 cagtgcggct agaagtgtgt tacgcgatga aattgccatg attgtctgct cccctgtctt
     4621 gttatgggag cagtattcag gaataaaaac attcattaaa agaattagtc gttatcgcac
     4681 agatgatttt attcttagca aaaaaacggc taaggaaaga ttaaatatgc aattactccg
     4741 ttctaacaat tgggaaaatt actacacttc attttagtga tgcataaaca accacattac
     4801 gagatttatt gcttcat
//
LOCUS       DAZFWP020000251         4781 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFWP020000251 DAZFWP020000000
VERSION     DAZFWP020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.253"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            78..791
                     /gene="ydiV"
                     /locus_tag="ACHK0Z_RS24055"
                     /old_locus_tag="pgaptmp_004811"
     CDS             78..791
                     /gene="ydiV"
                     /locus_tag="ACHK0Z_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4504676.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            854..2290
                     /gene="selO"
                     /locus_tag="ACHK0Z_RS24060"
                     /old_locus_tag="pgaptmp_004812"
     CDS             854..2290
                     /gene="selO"
                     /locus_tag="ACHK0Z_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU4504677.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(2294..2485)
                     /gene="hemP"
                     /locus_tag="ACHK0Z_RS24065"
                     /old_locus_tag="pgaptmp_004813"
     CDS             complement(2294..2485)
                     /gene="hemP"
                     /locus_tag="ACHK0Z_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU4504678.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2617..3663)
                     /gene="aroH"
                     /locus_tag="ACHK0Z_RS24070"
                     /old_locus_tag="pgaptmp_004814"
     CDS             complement(2617..3663)
                     /gene="aroH"
                     /locus_tag="ACHK0Z_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU4504679.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3820..4653)
                     /gene="ppsR"
                     /locus_tag="ACHK0Z_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             complement(3820..4653)
                     /gene="ppsR"
                     /locus_tag="ACHK0Z_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU4504680.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1178 a         1134 c         1222 g         1247 t
ORIGIN      
        1 tctgtaattg ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt
       61 cgagagggac ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt
      121 tcctgccgat aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct
      181 ccagtgaaga tggcacggtg cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg
      241 agcaacattg gcaattattt tcagagcagt tggaattact aaaatcatgc caacattttt
      301 ttattcaaca taaactcttt gcctggctta atttaacgcc acaagtggct acattattat
      361 tagagaggga taattacgca ggcgaactat taaaatatcc gtttattgaa ctgttgatta
      421 atgagaatta cccgcatctc aatgaaggaa aagataatcg cggtctttta tccttatcgc
      481 aagtgtatcc ccttgtgtta ggtaatttgg gagcaggcaa tagcacaatg aaagcggttt
      541 ttgatggtct tttcacccgc gtcatgctgg ataaaagttt tattcagcaa caaatcacgc
      601 accgctcctt cgaaccgttt attcgtgcga tccagtcgca aatttcccct tgttgcaatt
      661 gcatcattgc gggaggcatt gatacggcag aaatattggc acagattacc cccttcgatt
      721 tccatgctct ccagggatgc ctgtggcctg ccgttcccat aaatcagata acgacgttgg
      781 ttcagcgata acccttctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
      841 aacaggagga tctatgaccc tgtcttttat tacccgctgg cgcgatgaat tgccagaaac
      901 ctatacagca ctttccccta cgcctttaaa taatgcccgg ctgatttggc ataataccga
      961 actcgctaac acgctgagta ttccatcgtc gctgtttaaa aatgctgcag gtgtctgggg
     1021 cggcgaaacc ctactgcctg gtatgtcacc actggcgcag gtttacagtg gtcatcagtt
     1081 cggcgtctgg gcgggccaac tgggtgatgg gcgcggcatt ttacttggcg aacaactgct
     1141 tgctgatggc actacaatgg actggcatct gaaaggtgct ggcctgacgc cttattcgcg
     1201 aatgggtgat ggacgggcgg ttttacgttc gacgatacga gaaagtctcg ccagtgaggc
     1261 gatgcattat ctgggcattc cgacgacccg cgcgttaagt atcgtcacca gcgattcgcc
     1321 agtgtatcgg gaaacggtgg agccaggcgc gatgctgata cgtgtggcac caagtcatct
     1381 gcgctttggt catttcgaac atttttacta tcgccgcgag ccggaaaagg ttcgtcagtt
     1441 agctgacttt gccattcgtc attactggtc acatcttgaa gatgatgagg acaaataccg
     1501 tctctggttt aacgatgttg tcgcacgtac cgcatcgtta attgcccaat ggcagacggt
     1561 cggctttgct catggggtga tgaataccga caacatgtcg ctgctggggc tgacgcttga
     1621 ttacgggccg tttggttttc ttgatgatta cgaacccggt tttatttgta atcactcgga
     1681 tcatcaaggg cgttacagct ttgataatca acctgccgtc gcgttgtgga atttacagcg
     1741 tctggcgcag acattgtcac catttgttgc cgtagatgcc ctgaatgagg ccctggacag
     1801 ctatcagcag gttttgttga cgcattatgg acaacggatg cggcagaaac tgggcttcat
     1861 gacggagcaa aaagaggata acgcgctact gaatgaatta ttcagtctga tggcgcgaga
     1921 gcgcagcgat tatacccgca cattccgcat gctgagtctg accgagcagc acagcgcggc
     1981 gtcaccgcta cgtgatgagt ttattgatcg tgcggcattt gatgactggt ttgcccgtta
     2041 tcgggggcgt ttgcaacaag acgaggttag cgatagtgag cgtcagcagc tgatgcaaag
     2101 cgttaaccca gctctggtgt tgcgcaactg gttggcgcaa cgggcgattg aagcggcaga
     2161 aaagggtgat atgacggaat tgcaccgcct gcatgaggcg ttgcgaaatc ctttcagcga
     2221 cagagctgat gactatgtca gtcgtccacc tgactggggt aaacggctgg aagtcagctg
     2281 ctcgagttaa aggctacttg gtcaacagca gcttgccagc ctgagtttta cggagcagat
     2341 attcttgccc gtcatgatcg ataatcagtt tgccatccgg acctaacagt gtctggctgg
     2401 aaattcgccg aataggctgc ggggaagcgg tcttgtgatt ggcatccgtt tcaggcgtga
     2461 gttttctgct atccgtataa cgcataaaaa ttaataataa ttgatataac aatgattctt
     2521 attatcaata ttatggctga ccagagcaag cagtaatgtg ctgagaagac aacaagttgc
     2581 gcagaaatgc ccatcccatc aggaatgggc acgcattcag aagcgggtat ctaccgcaga
     2641 ggcaagtttt tcgaccaggc gttcggtatc ttcccagccc agacacgggt cggtaatgga
     2701 ttgaccgtaa gtgagcggct gaccgccgac gattttttgc gttccttcgc gcaggaaact
     2761 ttccgccata attccagcaa tcgccgtaga gccattgcgg atttgctgac aaatatcctc
     2821 acaaacttct aactggcgac ggtgctgctt ctggcagtta ccgtggctga aatccaccac
     2881 cagatgttca ggtaaatcaa actcgtgcag cgtatcgcag gctgcggcga tatcatcggc
     2941 atgataattc ggttttttgc cgccacgcat aataatgtgg ccatacgggt tgccgctggt
     3001 ctgatagatg gtcatctgac catttttgtc tggcgagaga aacatatggc tggcgcgggc
     3061 tgcgcggata gcatccacgg caatccgcgt gttgccatcg gtaccatttt taaaacctac
     3121 cggacaggag agtgccgaag ccatttcacg gtggatctga ctttcggtag tacgtgcgcc
     3181 aatcgcgccc caactgatta aatcagcaat aaactgaccg gtcaccatat cgaggaactc
     3241 agtcgcggta gggacaccca gctcatttac ctgtaaaagt aatttgcgcg ccagctccag
     3301 accgtgattt acccgatagc tgccgtttaa atccggatcg gagattagcc ctttccagcc
     3361 gacaacagtt cgtggttttt caaaataggt gcgcattacg atttccagcc gtgactggta
     3421 ctggttgcgc agcgactgca gacgggtggc gtactccatt gcagcggtga gatcgtggat
     3481 cgagcagggg ccaataatga ccaacagtcg cttatcttca ccattcagta ttttttcaat
     3541 tctgcggcgg gagtcggtga catgggtggc gacgccaggc gttacgggat accgtagcgc
     3601 gagttcggcg ggcgttacca ggctctcaat acgcgcagta cggagttcgt cagttctgtt
     3661 cattacaagt ctcagaaatt tgttgcttct acggcaggac taccgggaag tgatgcgcat
     3721 cacgataaac caatcgcagc tgatttcaag tgcgaggtgt gtcacacctc gccgggtggt
     3781 tagcatgata acaaaaaaat aagctaatgc actagttctc tagtacattc ggcggctaag
     3841 ccccatgata tcgaggatct tggtggcaat ctcttctacc gaataattgg tactgttaat
     3901 ccacgggatc tgatttttac ggtacaacgc ttctacttcc gcgacttcca tcctgcactg
     3961 acgaagcgag gcatagcggc tgttctcccg acgttcctcg cgaatcgccg ccagacgttc
     4021 cgggtcgata gtcaggccga acaatttatg ctgaagcggt ttgagcgacg cgggtagcac
     4081 cagattgtcc atatcgtcgg caataaaggg gtagtttgcc gcgcggatac caaactgcat
     4141 tgccagatac agactggtgg gggttttacc acagcgagaa acaccgagca ggatcacctg
     4201 agcctggtcc agattgcgca acgaaatgcc gtcatcgtgg gcgagggtgt aatcaatcgc
     4261 cgcaatgcgc gcatcatatt tattgagatt attagggtta aggccatggg tacgatgagc
     4321 aatcggcgtt ggatccagtt tcatctcttg ttgtagcggg gcaaccagcg cctgaacaat
     4381 atcctggcaa aagccttcac tttgcaagat gatggcgcga atctccggca acacgatgga
     4441 gtagaagacc agcgggcgca cacctgtctg gtgataaatt gcgtcaatct gatccttcac
     4501 tgcccgtgca cggctctcat tttcgacaaa cggcagcgta atgctgctga tagtgacggg
     4561 aaattgtgac attactgcgt gtcctaatac ctccgcagtt attgccgtac catcagaaat
     4621 ataaaaaacg tggcgatcaa cagcattatc cattttgttc ttcccgtgat gcagacataa
     4681 ttctatgaaa gcataaatta aaaacgcaca gaagcgtagg acgtaatgtc tggtttataa
     4741 aatgaacctt caattttatt ttttatgaaa acagcatttc a
//
LOCUS       DAZFWP020000252         4676 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFWP020000252 DAZFWP020000000
VERSION     DAZFWP020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.254"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(129..1253)
                     /locus_tag="ACHK0Z_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     CDS             complement(129..1253)
                     /locus_tag="ACHK0Z_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HGU4504681.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(1983..2180)
                     /locus_tag="ACHK0Z_RS24085"
                     /old_locus_tag="pgaptmp_004817"
     CDS             complement(1983..2180)
                     /locus_tag="ACHK0Z_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4504682.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(2246..2516)
                     /locus_tag="ACHK0Z_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /pseudo
     CDS             complement(2246..2516)
                     /locus_tag="ACHK0Z_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2745..3017)
                     /locus_tag="ACHK0Z_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             complement(2745..3017)
                     /locus_tag="ACHK0Z_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504683.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWEAIYRSTSHYGKNVLLLQLA"
     gene            3337..3531
                     /locus_tag="ACHK0Z_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             3337..3531
                     /locus_tag="ACHK0Z_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504684.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            3874..4002
                     /locus_tag="ACHK0Z_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             3874..4002
                     /locus_tag="ACHK0Z_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504685.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(4312..4647)
                     /locus_tag="ACHK0Z_RS24110"
                     /old_locus_tag="pgaptmp_004822"
     CDS             complement(4312..4647)
                     /locus_tag="ACHK0Z_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504686.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1395 a         1074 c          965 g         1242 t
ORIGIN      
        1 cgtcagataa ctctttctgc aatattatta tgggagacta tctcatttat ttagtgatgt
       61 ccttcattta tgtgatgaag gacattatat tactcataac atttctcgtt gaaaatttca
      121 gcaatacgct acaattgttt cccgttgttt tttaattttc tcatcaaaac ctctgcaatc
      181 tctgcaggac tgggttgttc agccatttca gcggctacct gatctgaaca gaagcgtagt
      241 gaatcatcat aaagaaggcg atttaccaaa tcactggtca gtccatgctt gtccggaata
      301 atcccacacc cacgcttcgc aacaatttct gcattaacag agcgatctgc accttcgcca
      361 aacacaatct gtggtattcc actatacagc gctgtcaggg tatttcctgc gccaccgtga
      421 tgaataaatc catcagctcc attaaggaat acacccatag gtatccagtc aaccagacgt
      481 acatttgatg gtagttttcg taaaccagta cgagcattta ttgcaagttg caaaatgata
      541 tcagcatcaa cttcatttgc agaatccata acccatgaaa tcagctccag accatcaacc
      601 attggtttta cagtgccaag gctgataagt aaacgtttcc gatcagaatc cctgtcccac
      661 catgtttcct ttacagcacc tccgttataa ggaatatatc tcattgagat aaccggttct
      721 ccagcatttt taagaatgct catacttggg ggagcaacat caatccatgc taaatcacat
      781 agtggaccgc tgactccatg ccgcctgtaa gcattgctta aagaacggtt taacatctga
      841 atatgggcag atgtatgcgc gaatccaaca gccagcatca ctgaaggaat tctatattta
      901 gcagcaacca atgggcctgc cggatcaaga gggggataga ctattaaatc tggcctccat
      961 tttcctgcaa aatcgatgag gttatctgcc atttcatcgc taaaaaaaga gaaatgacca
     1021 ataatattac tttttttcct caactcttcc tgatggcgat atccagcctc tgaatctaaa
     1081 cctggaactg catcaaaaac aaccagtcca gcttctgctg ctttattcgc gaatttgcca
     1141 gcgctactaa ttactacatc atgtccaatt acacgaaagg cctgagccag agaaatcaat
     1201 gggtataaca aaccgtacag cggtgggcca ataaaaagaa tacgttttcg cattaaaaaa
     1261 gtccgtcttc tgcttacctg tttatttttc tatctctaag aaaacataat taatattatt
     1321 tttatgtgag cttatgcaca aaaataacca agtatattat cttctggtag agattcagga
     1381 tacagtttgt aggagctttc ttttttgaac aatgatttgc aggacagtta tgctaaacta
     1441 gcggaatcag tatcacaaca ataaatgact gatcatacct gaaatttgaa ttaacaggca
     1501 tcatgaatgt caatagtcta agcggtgact acttatatcg cttgcataaa tactaaacac
     1561 tgactagaaa agtattggat aaccccgtca ctaaaagtaa cgcttcggtt atttgctcgg
     1621 aatgtattaa cgccaagatt gttagccgtt acgccttata tcactggcgc tgacaaatgt
     1681 ttcttttcta atttactgaa ccggcgcaga cacgcaggaa agcacgccgg caccggtgta
     1741 atgccagttc taatcaataa gaatggaatg acgaatcagt caggaaggga taacaacccg
     1801 ctgctgaacg gaaggtatca gcaggaagat gaacgggaat atcgccagca ccaagggcag
     1861 ggccgggttc cacaatacca gcgctggcat tttgcacatt gccagcgtac cggcattaca
     1921 acacgtttca tgctgctgcc ccccaggatt cctcgattcg ttgctacatc cactgctcta
     1981 cttcacttcc actgctccac ttcacttttg taccagcggc tgctgcgccc cagtttgatg
     2041 ggtttaggga aatggccttc actgatcaac ttgtaaaacc atttgtcggt cataccggtg
     2101 aaactggtga tatagttcat gtcgatcagg gaattatcgg cacgcagaaa tacgctgtcg
     2161 tggcttgttg tagtcaacat ggtcagaatg tcctgtgaga tttatgaaga ttgtgcgaat
     2221 gcgggaaatc tactgagctg tgctttcaga actggcctgt tacgggatac cagggattac
     2281 cggcggggta acgggcttcc ggatcataca caccacgatt atcgcggaca aaatcactga
     2341 acgcccatat cacctcttta agtatgtctt cgcagtccgg taaatgacga tccagcgcca
     2401 catcccgggt ggtacagctt tgatgcgccc ggtgacacaa agcccggatt gttccagaca
     2461 tcctgaatca aacgccccag attaggggcg tcgaaatatg cctctctgac cattatattc
     2521 cggcgtatag gtagcaggtc tgaagtgaca atgcgtcacc tgacgttaaa agtcacccaa
     2581 gatgacccca gtgggggcaa caagtataaa accgtttttt gattaagtgg tcggatatga
     2641 aaaggggaag gtttatgtga aaaatacctg tgttttcttt gaaaacacac aacagtatca
     2701 ggttatttat gtttatacag tgcgttccaa actgacctga ataattacgc aagttgcaac
     2761 aataaaacat ttttgccgta gtgtgaggta ctccggtaaa ttgcttccca ggtgcagaca
     2821 atgactaacg aagaactaca ggcaatcgca ttatccatgc ttgagcgcca gcgcgaaaaa
     2881 gtacaccagc aacgagagtc tttgcttaac ccatctccat tcaccaggcc aaaacgattg
     2941 gagcttgaag cattcttgga caaataccca cggcggctta aatcaggcaa aaccagacca
     3001 ccaggctgat agctcacagt ttgttctccg attcaataaa acggataaga accatcagcg
     3061 cggtagcgta aggcactaca gcgcattgct ttacgagtaa taacaatgga tttatcacca
     3121 gaagacgcaa agtttttacg agaactgcaa caccgtatgc tcagagaagc cattatcgaa
     3181 cagcaaacgg gcatgagcgc catagaccca ttgcgataca aacgggcctc atctccttta
     3241 gttgacgaca attccaatgc ggataaaaca cgtccaccac actgattaca tttttcatta
     3301 gcacagtggt gtgatgcagc atacgaggaa aaccggatga atgagaacaa aataaaaagg
     3361 ctggagcaac tgttacaggc acgccaacgg gagtttgcca ctaaatgggg acgtgccgcg
     3421 ctgccacatg aacgcctaac ctattttcca cttgaagacc tcattaaaaa taatggcaaa
     3481 aaaatcaggt ctgataagaa acacttctta gaatgcaatg aagacaaata acaacaatct
     3541 gcatattgcc accaaccaca gatgtggcat cgttatcagc gcacttgatt ttctttatat
     3601 ccactgacga gtttgccggt cattctggta tcatgcagat atttacggcg tacaagcaca
     3661 ctagcatgac attgtaataa ggcctgagcc taacgcttta atgtgtgtca tacactcagt
     3721 ggcgtaatcg ttgacaatat ggtgcatgta atcgtaagcg tccagcgaaa tccatctggt
     3781 cataatctga agcatccgac aaagtggtgt ccaccaaata agtgatctgc gcccaatatc
     3841 ctgagccgga actttgacaa aggccaacga aaaatgggag cggatgttac agagttctcg
     3901 gtacagggga aaagtggtac ctgtcgccag ttctccgatc tgtttaaccg ggaaattatc
     3961 tcctacagct tgtcagaaag gtcggtgatg gagcatcgtt aatacgatgc tatacgatgc
     4021 attcacagtg cccggccaga ggatgccccg tcgctgcata tggatcaggg ttggcaatat
     4081 cgaattgcag gctataggca aagttaaagc cccatggagt agcacaaaat atgccgcaca
     4141 aaggaaacgg tctgaataac gcagtgatga agaacttctt cagcacactg ctaaaacgca
     4201 tagtgatcga gcgctgattc tggcgaacaa ctgaactaat acatcagaat ctgcattatg
     4261 ttaaataaat ataaaaagat ggtttaaata ccccgttact tttgacttac actatacggt
     4321 atcgcatcgt ttaatattcg caccggccag atttttattt ctattagttg tcacaatact
     4381 gaatgcgtac gaccacagta ttctggctcc tgtgtggtta tgctttaatt ctgcgttccg
     4441 ggcagataag cagttgcttg caggaatcct tcttgtgtta atgtcagttc cccttttacc
     4501 agtgctgatt tccacattcc gtccaacaga gcttatagcc tttccctgga ttatagcatt
     4561 gtccggctga agttcttttt gaataataat agaagcactg ctggcagatc cagtccgttt
     4621 ttcataaccc actgtactga taaccataat ctaatcagta gaaattgagt cgaaaa
//
LOCUS       DAZFWP020000253         4639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFWP020000253 DAZFWP020000000
VERSION     DAZFWP020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.255"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..301
                     /locus_tag="ACHK0Z_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             <1..301
                     /locus_tag="ACHK0Z_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504687.1"
                     /translation="YISIEIFNETFDNLRSRVESAFDKYLKFEENKEIKDNADSIKSS
                     ITGLIDKAKKRMRTVKYLASAMFYGGLIYVGYKQFPVFFEKSAVNLFYFLCHTIS"
     gene            complement(281..637)
                     /locus_tag="ACHK0Z_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             complement(281..637)
                     /locus_tag="ACHK0Z_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4504688.1"
                     /translation="MRDMYEVMDRWGAWAASDNSGVDWQPIAAGFKGLLPHGKKSRIQ
                     CDDDEGIMIDSCVARLRRYKPEEYELIIAHFVIGISLRTIAKKRKCSDGTIRKELQTA
                     MGFVDGCLAMLAYSMA"
     gene            complement(655..1644)
                     /locus_tag="ACHK0Z_RS24125"
                     /old_locus_tag="pgaptmp_004825"
     CDS             complement(655..1644)
                     /locus_tag="ACHK0Z_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4504689.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAATQPLADDPAVRTVFRNEAVIRRAGGVECHESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(1652..2449)
                     /locus_tag="ACHK0Z_RS24130"
                     /old_locus_tag="pgaptmp_004826"
     CDS             complement(1652..2449)
                     /locus_tag="ACHK0Z_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4504690.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNMAPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSTPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2469..2858)
                     /locus_tag="ACHK0Z_RS24135"
                     /old_locus_tag="pgaptmp_004827"
     CDS             complement(2469..2858)
                     /locus_tag="ACHK0Z_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4504691.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKPAGAAA"
     gene            complement(2855..3181)
                     /locus_tag="ACHK0Z_RS24140"
                     /old_locus_tag="pgaptmp_004828"
     CDS             complement(2855..3181)
                     /locus_tag="ACHK0Z_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4504692.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLVGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(3178..3831)
                     /locus_tag="ACHK0Z_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             complement(3178..3831)
                     /locus_tag="ACHK0Z_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4504693.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            complement(3831..4325)
                     /locus_tag="ACHK0Z_RS24150"
                     /old_locus_tag="pgaptmp_004830"
     CDS             complement(3831..4325)
                     /locus_tag="ACHK0Z_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4504694.1"
                     /translation="MSMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHYCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLQKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(4322..>4639)
                     /locus_tag="ACHK0Z_RS24155"
                     /old_locus_tag="pgaptmp_004831"
     CDS             complement(4322..>4639)
                     /locus_tag="ACHK0Z_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfO"
                     /protein_id="HGU4504695.1"
                     /translation="AAECDGEVVRPKEPNWTAWANEIRLMCVQDGRNHKQICEMYSRV
                     SRDPFWCRNVLSPSKLREKWDELSLRLSPSISTYTEKREDPYFKSSYDNVDYSQIPAG
                     FRG"
BASE COUNT         1085 a         1326 c         1090 g         1138 t
ORIGIN      
        1 ttacatttct attgagatat tcaatgaaac attcgacaac ttacgttctc gagtggagtc
       61 cgctttcgat aagtatttaa agtttgaaga aaataaagaa atcaaagata atgcagatag
      121 tattaagagt agcattacag gtttgattga taaagctaaa aaaaggatga gaactgttaa
      181 atatctcgca tcagccatgt tttatggagg ccttatttac gttggatata aacagttccc
      241 agtctttttt gaaaaatcgg cagtaaatct attttatttt ttatgccata ctataagcta
      301 acattgctaa acagccgtca acaaacccca ttgcagtttg cagttccttc ctaattgtgc
      361 catcagagca ttttctcttc ttcgcaatag tgcgtaatga gataccgata acaaagtggg
      421 cgatgatgag ctcatattcc tctggtttat accttctcaa ccgagccaca caactgtcta
      481 tcatgatgcc ttcgtcatca tcacactgaa tccgtgactt tttgccatga ggtaaaagtc
      541 ccttgaaacc agccgctatc ggctgccagt ccactccgct attgtctgaa gcagcccaag
      601 ctccccaacg atccattact tcatacatat cacgcatcaa ctttctccac aaaatcaggc
      661 cagcacacca attgccagcg cacgatcgat aaaacgaaat atcaactcca gctgggagcc
      721 atacttctct tcaaatgcca cggtatccgc atgcagctcg tcgtgatgct ttctgcacaa
      781 aggcaacaca aagagatcat gcgcttttgt acccattccc ccctgaccgt ggcctatcag
      841 atggtgggga tcatcagcag gctttccaca acatgcacac ggctgcgtct taacccagcg
      901 cgtgtacttt tcattaaccc agcggcgacg ttttgggcgt aacataaaag actccggcga
      961 ctccggatcc actttcagcg ccagcacctt tttcgcctta tcctggatga tgctggtggc
     1021 aggaaccgaa ggcacaaggt cactttcccg ggtgacagat ggcacaacag gcttcggtaa
     1081 tctcagtgcc ttacgggctg cactttccgg taaggcatcc gccagatcat tacgaaccag
     1141 ccaccagcac agttccggca ttgtcacaac gtgactgtca tcaaaaccga gatcccgacg
     1201 cacgacagac aacacccagc gggcacagtt atccgttgcc attgattcca gccgttccgt
     1261 gaactggtca cgcagctggt tatcgcagtg ccagcacaga cgaattgctc ccggagcgtg
     1321 ccgcattgtg gtcatgttct cgctgtgcca gtcagaatga ggccactgac agcccttttc
     1381 acgaagtaac cagctttcat gacattccac gccaccagca cgacggatca ctgcctcatt
     1441 gcggaacacg gtccgaacgg caggatcatc cgccagcggt tgtgttgccg ccggaacggc
     1501 accactggcg aaagatgaat aacgttccgg ctctggctcc agcaggacac gcccctgcat
     1561 aaacaggggc atcagctctg aacctggcct gaacaatacg atccccatac gcggggcaat
     1621 ttcaggggtc agtagtgctc tcacggtcac ctcaatgaac ggtatcgagt agctttaaca
     1681 gctcagggaa tcgggattcg aagaaatgcg gctgcgtctc gcgcggattt gcaggactgg
     1741 tgatgttctt gccgaacatg cagcctttcg ccgtcagcga ccagaatttt ttgatgttgt
     1801 taatcgcggt acggctgtat cgttcgcgtt gttcaacgat ccccagcttc gccatctggt
     1861 gatatgcctg attagctgtc aggcggatac catactgctt cagcagtgca ctcagtgaca
     1921 gcgtggggcg gcttgagcca tcaggcgcgt cagcaggagc atcaatggca tagcgcggtg
     1981 ccagattcgg taagccaaca gcctcctgga gtttctgaca ggcaccaagc actgaagagt
     2041 tagacagatt taactcccgg cgcataaagt ccagcaggat cacgccagcc tgcatcttgt
     2101 cagcagcctg cccggataat ttttccggtg tgctggttac catatcgaaa gtacggatca
     2161 ccttcagatg gaatgacggg ctgatccaca ttgcataggc atacaccagt tctttgcaga
     2221 catacgtccc ttggttattt ccgccacgaa taacgttaac tggcgccata ttgaccgagt
     2281 tgcaaatttg caactcgctt attaaacgtt cggtttgctc attgcggagc cagaatgcag
     2341 gcttatgttt atccagagaa ccggcagccc tgtgcagatc gttcaggctg taacgcccat
     2401 aagcatcacg acgaacttca ataccatcaa taaccatcag attattcata cttcgtttct
     2461 cctcttaatc aggcggctgc acccgccggt ttctcgtact tactgatagt gatctcgacc
     2521 ttcccttccg ggataaccgg tccccactcc accagcattc ttttcacctg actgtcgtct
     2581 tcccacacac ccgcgtgggt cagggcgtca aacagcgcct tgttatagtt gtccagatcg
     2641 cggatccggt tatccggagg aaacaacacg atctccactg aagcaggtgc cgacgttggt
     2701 tttggcagac gacgtaactg ctcaactatt gctgcacacg ccgcgctctg gaatttgcgc
     2761 cccgccgcgc ttatcaggct cttacctgca aacgcccctt tgttggggtg tcgccagtac
     2821 gtgttcacgc tgggcgggaa aggcaggatc agcttcatac tttcaggccc ctctcatgta
     2881 accagtgggc tgcacgcagc ctggcgtttt cctcaccgac aagcagtgag cggataatcc
     2941 cgaccgcctc gctgtcgtcg tccttcaccg cggtatgaag cgtgatgccc cgggccacgc
     3001 cacgctttat cgtgatgacg ccttttttct ccagtgcgcg aagatgttct accgctgcat
     3061 tcactgaacg gtatcccagc atggttgcca cctcctgatt ggttggcggg aagccacgtt
     3121 ctttctgata agaaatcagc atatccagca cctgctgctg gcattgagtt aacgtcgtca
     3181 tgccaccatc tccctgacca gtttttctgc ctgctggcga acctgcgcca gaaaggcctc
     3241 accacatgcc tcaagttcat cgcgcccgat gtagctgatt gccggtccct tccaggtctt
     3301 gtcgaaaaca gcaatagcac cagcgaagaa agcgcctgtc ggcacctgct tctcatcctt
     3361 cgggataaac caggcaggca gttcaaaacc gatacgcccg cgaataaaag caatatgatc
     3421 tgcatcttcc ggccaccaca cttcgctggt ggcagctttg atcaggaaaa catagcgccc
     3481 acctttatca cgcatggcac tggcatgctt catgatgtaa cgcatgcctg tgatgtattg
     3541 cccctcatgc tgactggcgc ggctgtatgg gggattacca aaggcagcac ctttaagctc
     3601 cgcaagacgt tctgaccagt catgcgccag cgcgttgtct tccgccgtgt aatacgcggc
     3661 acatttggcg ttatcaccgt cagtgaacag atccagaaca aacgggccaa acagggtgtt
     3721 aattccccag aaaatgttat ccggcgtgcg ccactgatcg cccacttcct tcagttcatg
     3781 ggctggtttg ttccgcagtt ccaccagcgc ctggcaatat ttattactca ttaagccccc
     3841 acgtaattcc ctgacagata ccactcttca cccgatgcag cgcgcttgct gcttttctgt
     3901 aagcactgct cacgacgcgc cagaaaattg tttcgttctg gctgggagtg gctttcacgg
     3961 aatgccgcca tccacaccgt tgcagcacga cggtataggc ccctggactc cagttcttca
     4021 gcctggcggg tcaggcacaa aatcacccgt ggatcgttag tgccgacata gaaattgcgc
     4081 acaggtctgg tttcacgaac tggttgcggt tccggctcct gcgctctctc agtcaggcgc
     4141 gggaaatgtc tgcgtgtatc cccttcacaa tagtgagcca cacgcccact ctgacgtaac
     4201 ttgcttgctg actgcagaac gcgctgccgt gagtaacctg caaaagcatc cgcaatgtct
     4261 ccggaagtac agcccggatg ggcttcaatg aatttctgaa cttcattcaa aagactcatg
     4321 ctcaccccct gaatcctgcc gggatctggc tgtagtccac gttgtcgtaa ctggatttga
     4381 aatatgggtc ttcgcgtttt tctgtgtatg tgctgatgga cggcgataag cgcagggaaa
     4441 gctcatccca tttttcccgc agcttcgacg ggctgagcac gttacggcac cagaacggat
     4501 cgcgactgac gcggctgtac atctcgcaga tttgtttatg attacgacca tcctgcacac
     4561 acatcaggcg aatttcgttt gcccaggctg tccagttcgg ttctttggga cgaaccacct
     4621 cgccgtcaca ttcggcggc
//
LOCUS       DAZFWP020000254         4525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFWP020000254 DAZFWP020000000
VERSION     DAZFWP020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.256"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..348)
                     /locus_tag="ACHK0Z_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             complement(<1..348)
                     /locus_tag="ACHK0Z_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4504696.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTVGVV
                     EQNLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAG"
     gene            complement(329..742)
                     /locus_tag="ACHK0Z_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             complement(329..742)
                     /locus_tag="ACHK0Z_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4504697.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREKAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVADVSEDDVALMMASAGIPGGVRYVPAGW"
     gene            complement(769..1200)
                     /locus_tag="ACHK0Z_RS24170"
                     /old_locus_tag="pgaptmp_004834"
     CDS             complement(769..1200)
                     /locus_tag="ACHK0Z_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4504698.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHADTDSTEVAKYNALTDDDFSAGKSSTAS"
     gene            complement(1214..1966)
                     /locus_tag="ACHK0Z_RS24175"
                     /old_locus_tag="pgaptmp_004835"
     CDS             complement(1214..1966)
                     /locus_tag="ACHK0Z_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4504699.1"
                     /translation="MSTPNPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWLRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRVVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(1974..2369)
                     /locus_tag="ACHK0Z_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             complement(1974..2369)
                     /locus_tag="ACHK0Z_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4504700.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(2366..2899)
                     /locus_tag="ACHK0Z_RS24185"
                     /old_locus_tag="pgaptmp_004837"
     CDS             complement(2366..2899)
                     /locus_tag="ACHK0Z_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4504701.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            complement(2914..3267)
                     /locus_tag="ACHK0Z_RS24190"
                     /old_locus_tag="pgaptmp_004838"
     CDS             complement(2914..3267)
                     /locus_tag="ACHK0Z_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4504702.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(3260..3643)
                     /gene="gpFI"
                     /locus_tag="ACHK0Z_RS24195"
                     /old_locus_tag="pgaptmp_004839"
     CDS             complement(3260..3643)
                     /gene="gpFI"
                     /locus_tag="ACHK0Z_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4504703.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRQCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            complement(3695..>4525)
                     /locus_tag="ACHK0Z_RS24200"
                     /old_locus_tag="pgaptmp_004840"
     CDS             complement(3695..>4525)
                     /locus_tag="ACHK0Z_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4504704.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGK
                     KWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
BASE COUNT          997 a         1435 c         1049 g         1044 t
ORIGIN      
        1 accggcaagt cccctgaaca tggggatcat cccgccgaag gagtccttca cctgaccgcc
       61 ctgttgcagc aggatcagcc agggattctg accaccggca agctgcgtgg cgatatccgt
      121 aaactgtgcg ggcagggttc gcatggccgc tttatactgc ccgacggaaa tcccggcttt
      181 ttgtgcagcc agcgcctgac gactcaggtt ctgctcaacg acaccgacgg tttttctggc
      241 gtcagtctcc agtccggaaa aatgacgcct tacccggctc atctgctcat cgaaacggac
      301 cgcatccaga ctcaggtcaa taacaagatc accaaccggc tgggacatat ctcacacctc
      361 ccggaatccc cgctgaagcc atcattaatg cgacatcatc ctcgctgaca tccgccacat
      421 ccgcagacga taaaatctca cgccctccgt ccccaccaaa ccgtacgcct ccggcaagtc
      481 ctgccgcttt ctgcatcagc attttttcct catccggcat ctccgtctgc gctttctcac
      541 gccggggggc aagcagactg aaatcagagg gatgcatatc cggatcgcaa aaaaacaggc
      601 tgagtacggc gtacgtcagc ccggaaaaat gcatatccag ttgggtatcg tgaaaataat
      661 gcgtacggta aaaatgtcgc cagtcggcat attcggtgga tgtcatcccg gcaagcatgg
      721 cgcgccagtc aggcctcccc atctcacgcg ccagtctgag ggcaaaattc agctcgccgt
      781 cgaagacttt cccgcagaaa aatcatcatc agtcagcgcg ttatatttcg ccacttcggt
      841 gctgtcagta tccgcatgaa cagccccgct catcccggac agacgcaaca ccacatcttc
      901 cgcccgggca atggcatccg caggccaggt ggtgagcacc tcctgttcga tctgcatcac
      961 agcctcattc attgacggtg atgccgtttt ctgcggatgg ttatgccaca gggacatcgc
     1021 caccagaaac gcgccggttc tgacgagatc ttccacgctt acctgcaggt tgccgctgga
     1081 ttctgcctgt tctgcacgcc gtttcaggag ggcaagatgc tcaatacgct gcagcgcaga
     1141 caattcggaa agcgtgacag acacaccgtt atattcaaat tgttctgttt tcagaaacat
     1201 gtattacctc cgtttaccct gcagcgcccg cttcagtaac ggtgacttca gccactgcgg
     1261 cgaactgacc atttccgctc accacaggga tctgcacctt acctgtcgcc acgccgttta
     1321 ccgtaattgt catatctttc acactaatgg tggctttcga cggatcggcg gaaaccactc
     1381 tgaacgtctt gtcggttgca ctttccggct caaaagaaac cgtcagggtg gttgttttcc
     1441 cttttgccac cgtaccggat gtcggcgtca ccttaatcgc agtgaccggc gtaattttgc
     1501 tgcgttcttc cgctacagaa ggtttgccca cgttggtcac tttcaccgtg cgggtgatca
     1561 cttctttcgc cgtcacggcc ttaccgatac tgctgaccca gccacgaaac acatccaccg
     1621 tgccgttcgg aaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     1681 gccctttctg accttcttct cccggtttcc aggccagcgt aaaactggta tctcctgcag
     1741 acttctgccc ctgcccggtc gcggtccagt ccgcgtcttc atcatccagg tagttatcat
     1801 cgtagggttc tgccgtcatc tcgcccggcg tcagatcctt caccttagcc agtcgcagcc
     1861 agtcatcgtc tgacaacggg tttgcatagg cgtcaccctg accgttataa acccacaggg
     1921 tggtaccggc accttttacc ggctcaaggg gatttggtgt tgacatatcg tcctcacatc
     1981 tcgtatgtaa tggaataagt cagatccgca gaactccata acgccatatc gtcatcacga
     2041 cgatactcat agccctgcgt aaccatcgtc gtaatcaggt ctgccagtgc cgggatcgca
     2101 gtcatcgccg gataaatccg gctttccatc cacgaatcca gctccgaatc cggtacctgt
     2161 gctggtaaaa acacctcaat atgcagtgtg gcccgccagg tatctgcatc cagctcttca
     2221 ccggtatact ctgcatccgt cagataaacc gcgacggccg gaaaatcctc ttcgtcaaaa
     2281 acaacggggc gaccatcaaa cagcgtcgcc ccgtgttcat gcagctccag tgcatccagc
     2341 actgccgcac ggatatcagt atgtttcatc gttttatcgc aatcctcagt tgttgtttca
     2401 gcgcatatgc cagttctccg ggcaggcgtt cacgccggat acggtcaacg ttttcatcaa
     2461 acgcctgttt cagtggggcc gccatcggga ttttcaccac atcaatgggg taacggtttt
     2521 tcccggccac acgctgcatg acatgccagc gaccattttt taatcgctga ataaatgccc
     2581 gctgataacg atgctgaccg gctttgagta tgctgtccgg acgacgcccc ggcatcctga
     2641 tccccagctt aatcacaggg agatcaccgc ggttaacgat aattctggca ttcggatttc
     2701 tgaccgtcgc ccgtttcagt ctggaccgtt ccttaaccag tttccggcga acctttgtct
     2761 cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt ggccacgcgg ttaatggcca
     2821 ttgctgaagc cgccggaatg gcgtttttac gaacccggct cagattttca atcgcctgat
     2881 caagcccttt tatcgccata attcaccctg cgtttatcgt cgccggttaa ctgccggtgg
     2941 ttgcccacgg ttgagccaga gataacaact gcccccgtca tccggagaaa cacgatccac
     3001 ccagaatgtc tcaccattaa tggtcagcgt gtcaccacgc cgcacagcac gaaccgtatc
     3061 cgtccgtaca aataatgacg ggcagcttcc ttcaatacgg accccgctac cggcaaaccc
     3121 cagcgactcc ggatcgtcaa aaaccccctg aacttcgctg ccacactgtg cccccgaggt
     3181 gaactgcgca cagagcccca tcacttcaac aatcgtgctg tccaccccgg caagggctgc
     3241 atcaaaggca ttctgaaaat cacgcatgct cagccgttcc gtgctgtatc atggctgtcg
     3301 ccagtggtga tggcaccaga acacgcatgc cccggtacgt cagttcaacg ggacggcctg
     3361 tctccgggca ataccccatc acatgcaggc attgccgcac acggaccgct ttaacatcat
     3421 ccgtagcatc agtgttgtgc aactgctcac catcgtctgt gtgattttgc tcaggcccgc
     3481 tctcatcacc aggcataatg ccctcccggg aagcagcaag ctcctcttcc cactcagaca
     3541 cacgttgcgc aatatccgca gcactccccg acatatccgc ctcgcgcccc agcaggccag
     3601 ccagttgacg aagacgtttc tgattttctt cttttgttgc catatcagcc ccctgtgaaa
     3661 aaagacacgg gggcatttcg cccccgctca cggattattt cacctgcacc accacaaact
     3721 catccgggtc cggcagcacc atcagcggtg cggactgcgt catggtaaat tcacgggccg
     3781 gatcgcccac ggtcagccag tgtttcgggt aacgggaaga agccaccaca ccttcggaca
     3841 acgcctgcgc atcctgaatg gcaccgtaac aacggatccc atctgcagcc gtatttccca
     3901 ggaccagcgt gccctccggc agataacgtt tttcggtacc gtcctctgcc acataagacg
     3961 ttttcgccac cacaatggcc agatcgccgt aatacccttt gaaagacacc accgccccca
     4021 ggtctttcac tgccgtttcg agttgtgaat ttgagccgcg acgggtatcc agtttttcgc
     4081 ggaacagctt aaaaccattc agcagacgcc agacagtacc gtccataatg gcaatattca
     4141 caagaccgct ggcctgatcg cagtagaggt caatatcatg cgtcggatca aacgtatcac
     4201 ggtcctgctc agaccatttt ttaccgtcgg cctgctcaat gttatttcct tcagagcgtc
     4261 caaaatccac ctcgacagta tcaaactgat ccccttccat ggtgtatttg ccatacagca
     4321 cggcattcac cgcctgcatt tcttccacct ggacaatggc atgctcttcc tgtttgaggt
     4381 tatcggtgat gatacgcaga cgacggtagg ccgggtcgtt cagttgagcc ggatcttcac
     4441 cgggaagacg ctcaaccgcc tgctggtaat taaattcgtg tttcggcttg acgtagcccg
     4501 gacgtaacac gcgggtttca ccacc
//
LOCUS       DAZFWP020000255         4525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFWP020000255 DAZFWP020000000
VERSION     DAZFWP020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.257"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..517
                     /locus_tag="ACHK0Z_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /pseudo
     CDS             1..517
                     /locus_tag="ACHK0Z_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            694..921
                     /locus_tag="ACHK0Z_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /pseudo
     CDS             694..921
                     /locus_tag="ACHK0Z_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1448..2818)
                     /locus_tag="ACHK0Z_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /pseudo
     CDS             complement(1448..2818)
                     /locus_tag="ACHK0Z_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2838..3185)
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS24220"
                     /old_locus_tag="pgaptmp_004844"
     CDS             complement(2838..3185)
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4504705.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRDRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3182..3856)
                     /gene="tnpA"
                     /locus_tag="ACHK0Z_RS24225"
                     /old_locus_tag="pgaptmp_004845"
     CDS             complement(3182..3856)
                     /gene="tnpA"
                     /locus_tag="ACHK0Z_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU4504706.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            4110..4525
                     /locus_tag="ACHK0Z_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /pseudo
     CDS             4110..4525
                     /locus_tag="ACHK0Z_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         1030 a         1097 c         1246 g         1152 t
ORIGIN      
        1 gctgataatc atacgctatc ggctggttat gctcagagta aggtacagga ctttaagaat
       61 attaaaggag tgaatctgca atatcgatat gagtgggatt ctccggtcag tgtcgtcggg
      121 agtttttcgt atatgaaagg tgattgggcg gacagtcatc gcgatgaagc tgacgatttt
      181 tacagacatc aggctgatat taaatattat tcatttctcg caggcccagc ttatcgtctg
      241 aatgactata tcagttttta tggtttggtg gggatatctc atactaaagc gaagggagat
      301 tatgagtgga gaaacagtgt gggtgctgat gagtctgatg gatatttaag tgagtctgta
      361 tcaaaaaaaa tccactgatt ttgcttatgc agctggtgtt attattaatc cgtggggcaa
      421 tatgtctgta aatgttgggt atgagggaac taaagcagat atatatggaa aacattcggt
      481 caatggattt actgttggag ttggttatcg tttttaatca ttagacggga ctggataatt
      541 gcacttgcgt agtgaagttt actgaatgtt tgtattttaa tgaagtattc gacatattcc
      601 cttcagacgg aatatgtcga cggttatggt tttactggag catgagtatg ttatggcgaa
      661 actgataaat atatcaggtc cactgaggag agaatgacgc caggggagag gagggtggct
      721 tcacgactgg agagctttct gaatgatgat tgctttgttt ggtacgatat cccggtggga
      781 cggaaaaacc gacatccgga ttttgtcatt attgacccgg ataacgggct ggtatttctg
      841 gaggtcaagg actggactgt atccacatta cgtaaagcaa atcaggagca ggttacactg
      901 gaaaccgacg gtcttctgaa atcagaaata aatccactgg tacaggttcg tcgttatgcc
      961 tgtgatacgg ttaatgcctt acctgctgac ccgtgcttac ggcagaatga cggtcagtat
     1021 aaggggcggc tgaacctggc ctgggcgtat ggtgtggtgt ttacccgcat tacccgtcag
     1081 caactgaagg cactgacagg gaataatgag aatgcggtgg aaaagatttc tccttcagca
     1141 cagacaatct gtcaggatga aatgacgcag tctgttttac ctgaggtatt ccggcaaaaa
     1201 attgccggga tgtttaccac ggggttcaga acccgggtaa caccccggat gagggatatt
     1261 ctgcgcgccc atctgtttcc tgaagtgacc gtgaaacaga actcacaaat caaaattatg
     1321 gatattcagc aggagattct ggccagaaat attggtgacg ggcatcgtgt cattcatggt
     1381 gtggccggtt ccggaaaaac catgattctt ttgttccgtt gcctttacct ggcagaaacc
     1441 acgtccggta gttacgccgc cccagtgcaa ctacccggag ggcgttttca cacaggttat
     1501 tgtcgatctc cacccagcca ttgcggcagt attcgttcag agcgtcccat tgcttcagca
     1561 gataggtgaa cgctttcgct gtatccgagt ggcgcgacag cgtgctcatc tgcccctgga
     1621 gccactcata caacgactgc attagcggta ccgttctggc ttttctgacc gccagtcgct
     1681 cttctgccgg actgccgcgg atctcagcct cgatagcgta cagttccccg atacgacgga
     1741 gcgcttctcc cgttactgtc gttggatggc ggacatgtac atcgtggatc ttgcgtcggg
     1801 catgtgccat acaagccgct tcggttacct gaccgctttc gtaaagagca ttgtaacccg
     1861 catatgcatc ggcctgcagg atacctctgt agtccgccag atgttgctgt gggtggatgc
     1921 ctttgcggtc gggagagtat gagaaccaca ccgctgccgg catggtcgaa ccggcgttgc
     1981 gatcgtcacg aacatagacc cacagccgtc cggtacgggt tttaccctga cccggctcca
     2041 gtacatttac cggcgtgtca tcggcatgca ctttacccgg catcagcaca tagtgcttca
     2101 gttcatcata cagcgggcga agctgctctc ccatgatgtc aacccagcgc cccatcgtat
     2161 tgcggtgcag ctccacgccc tggcgggcat agatttctga ctgacggtac agcggcagat
     2221 gctcggcgaa cttagccatg attatgcggg ccagcagagc cggactggcg taactgcgct
     2281 cgatgggttt tggtggctgc ggagcctgaa ctatacagtc gcaccggctg caggcccgtt
     2341 ttgggcgaac cgtttcgatt accctgaacg cggtgttgat gatatccagt tgttcagaga
     2401 tgctttctcc cagcggtttc agtttgccgc tgcagacggg gcattcggtt tctgccgggg
     2461 agataacctg cctgtcacgg ggaagtgttg ccggaagtgc tttgcggacg ggagagtctg
     2521 atgttttcgg cgctgtctct ccggccattg aggtgagttg caactgcgcc tcaccaagca
     2581 tgttctggag ctcggttata cgcgcttctg cccgtgcgat cttcttttct atcttctcgc
     2641 ggcttttctc gctgctgcga ccgaacaaca ttctctgcag tttagcgacc agcgctctga
     2701 gtgagctgat ctcgcgggca tagccggtta tttcaccaga cagacggacg ataacagcct
     2761 gctgtgcgat cagcagggcc ttcagttgct cgatgtcgtc ggggagtgtg ttgttcattc
     2821 ccctgtttta tcacgggtta tatccggatg ccaggccgtt ctgtccgttt gggatgttgc
     2881 catgcgatcc cctccagtag catggataac tgagctggcg tcaggtgcac tttcccttcc
     2941 cgggttaccg gccagacgaa gcggccccgt tccaggcgtt tggcgaacag gcataacccg
     3001 tcacgatcgg cccacagtat tttcaccatt ttgccactgc ggtcccggaa gacgaagata
     3061 tgcccggaga acgggtcatc tttcagcgtg ttctgcacct tcgaagccag gccgttgaag
     3121 ccacaacgca tatctgtgat gccagcgatg atccagattc tggtaccggt tggcagcgtt
     3181 atcatcgggt acctcctttc atttcgcgga ttagcgcccg taacatttcc ggagtgagag
     3241 ggtcaaacag ttttaccaca cctgatttaa gatgcagctc gcaccgtggg acgtttccgg
     3301 gatcactctc agggcactca tcaggcttgt tacgccagaa gggatttgta actggtctgg
     3361 tcggctctgg cgtatcagtc agagccaccg ggacaggcat gcattcctgt atgtcatcat
     3421 cgctcagtaa gccgtcctcg tactggcttt tccatttaaa cagcaggtta tcattgatac
     3481 cgtgctctct ggcgatccgg gcaacaacag caccgggctg taatgcctgc ttagccagac
     3541 ggaccttaaa ttcacggctg tagctggctc gccgttcttt tcgccatgtg ccttcgctga
     3601 tttgaggctc tgttaattcc ttctttctgt tggcataaag gatggcgtca agctgagcta
     3661 atgaaactga atcgggcaat ggccatgcga taccggatgc aataaatcgc tgaaaaagcg
     3721 tatgtattgt ggaatgactg agacctagac gctgagcgat ggcccggatg gtcagtttat
     3781 cttcaaatct taaacgcaga gcatcaggca aataagaacg gaagcaggga atatcttttt
     3841 ttgtctggga attcatcgtt cgtgtccatc tatatagatg ggcgcgattg ttgccagaca
     3901 ggacaatttt cacaagacgt cgcagatggg gcgcttacac aacaatggcg cgagggaaag
     3961 ctgctattac cttcttcaga gagcccccag ctacttcctg tgactctcga tgcagctgcc
     4021 gaacagccag aatcgctcgc agaggacccg gaaaccctca gtatcagctg ttgtcaacga
     4081 cggatgacta ttgcaccgtt cgtgacagtg tgctgcaagc ggatgcgtac gccgggttca
     4141 acgagctgta tcgcaatggt gggataacgg aagctgcctg ctgggctcat gcccgccgaa
     4201 agatccacga tgtgcacgtc cgcatcccgt cagcactgac ggaagaagcc ctggagcaga
     4261 tcggtcagtt gtacgccata gaggcggata taaggggaat gccggcagag cagcggcttg
     4321 ctgaacgtca gcgaaaaacg aaaccgctgt tgaaatccct ggaaagctgg ttgcgtgaaa
     4381 agatgaagac cctgtcgcga cactcagagt tggcgaaggc gttcgcgtac gcacttaacc
     4441 agtggccggc actgacgtac tatgcgaacg atggctgggt ggaaatcgac aacaacatcg
     4501 ctgaaaatgc cctgcgggcg gtcag
//
LOCUS       DAZFWP020000256         4510 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFWP020000256 DAZFWP020000000
VERSION     DAZFWP020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.258"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(32..490)
                     /gene="yaiI"
                     /locus_tag="ACHK0Z_RS24235"
                     /old_locus_tag="pgaptmp_004847"
     CDS             complement(32..490)
                     /gene="yaiI"
                     /locus_tag="ACHK0Z_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU4504707.1"
                     /translation="MTIWVDADACPSVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            610..1419
                     /gene="proC"
                     /locus_tag="ACHK0Z_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             610..1419
                     /gene="proC"
                     /locus_tag="ACHK0Z_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU4504708.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1436..2551)
                     /gene="adrA"
                     /locus_tag="ACHK0Z_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /gene_synonym="dgcC"
     CDS             complement(1436..2551)
                     /gene="adrA"
                     /locus_tag="ACHK0Z_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU4504709.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2653..2973)
                     /gene="psiF"
                     /locus_tag="ACHK0Z_RS24250"
                     /old_locus_tag="pgaptmp_004850"
     CDS             complement(2653..2973)
                     /gene="psiF"
                     /locus_tag="ACHK0Z_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU4504710.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAEKTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3092..4507)
                     /gene="phoA"
                     /locus_tag="ACHK0Z_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     CDS             complement(3092..4507)
                     /gene="phoA"
                     /locus_tag="ACHK0Z_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU4504711.1"
                     /translation="MKQSTIALALLTLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDHTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
BASE COUNT         1037 a         1244 c         1140 g         1089 t
ORIGIN      
        1 tcaagaatga agtgtaaaca ataaattaca tttagccacg actacgttgc acttccagcc
       61 accacttctc cagctccgcg gcaaaggcct ggcggtcacg ttgtgaaagg ctatctggtc
      121 cgccggtctg gatcccactg gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct
      181 cacgaatggt cgctggcgtg taacgttcgc cgcgcggatt aagcgccgct gcgcctttct
      241 cgatggcttc agcagccaaa ggtatatctg cggtgatcac caaatcgccc gcttcacact
      301 gccggacaat ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa
      361 atcgcgatgg cggcacgcgt aaactctggt ttgctaccag taccagcggc atctgcatac
      421 gttccgccgc gcgatacaaa atctctttaa ttacactggg acacgcgtcg gcatccaccc
      481 aaattgtcat aaagtcatcc tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc
      541 cggcatagtt ctgtttatgc ttctgccagc gattatcaaa acaatgaatt tcacggcagg
      601 agtgaggcaa tggaaaagaa aatcggtttt attggctgcg gcaatatggg aaaagccatt
      661 ctcggcggtc tgattgccag cggtcaggtg cttccagggc aaatctgggt atacaccccc
      721 tccccggata aagtcgccgc cctgcatgac cagttcggca tcaacgccgc agaatcggcg
      781 caagaagtgg cgcaaatcgc cgacatcatt tttgccgccg ttaaacctgg catcatgatt
      841 aaagtgctta gcgaaatcac ctccagcctg aataaagact ctctggtcgt ttctattgct
      901 gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa aattattcgc
      961 gccatgccga acactcccgc gctggttaat gccgggatga cctccgtaac gccaaacgcg
     1021 ctggtaaccc cagaagatac cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg
     1081 gaagtaattg ctgagccgat gatccacccg gtggtcggtg tgagcggttc ttcgccagcc
     1141 tacgtattta tgtttatcga agcgatggcc gacgccgccg tgctgggcgg gatgccacgc
     1201 gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa
     1261 acgggagaac atccgggggc actgaaagat atggtctgct caccgggagg caccaccatt
     1321 gaagcggtac gcgtactgga agagaaaggc ttccgtgccg cagtgatcga agcgatgacg
     1381 aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat gactttcgcc ggacgtcagg
     1441 ccgccacttc ggtgcggtta cgtccggctt tctttgcttt gtaaagcgcc aaatctgccg
     1501 atttcaacca ctcacgatag tgactcattt gtgggttcag cggcgcaacc cccacactaa
     1561 tccgtaaagt tacctgtggc gtattcggca agcgtaacgt gtttagccct tcacgcaccc
     1621 gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga catgattact gcaaactcat
     1681 cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa
     1741 gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg ctcttgaaat
     1801 ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt
     1861 cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcggg
     1921 tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga
     1981 cccagccaaa cagcagagga taaacgacaa taatgggaag ggagagccac cattccagcg
     2041 gcgcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg caggaaacca
     2101 ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac
     2161 acataatcat caacatagcg gtggaaggca gcacgtttac gcccattacg cctacccaca
     2221 ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg
     2281 ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacca
     2341 gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc
     2401 cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct
     2461 gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa
     2521 agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga
     2581 cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag
     2641 cactatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
     2701 acctttcaga gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg
     2761 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
     2821 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
     2881 ctgttgttgt ggggttaagg ttttctcggc agcgccgacg gtggttaaaa aaaccagacc
     2941 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
     3001 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
     3061 cggcagcgaa aattcactgc cgggcgcggt tttatttcag ccccagagcg gctttcatgg
     3121 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc gggccatacg
     3181 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcagag ttcccgtaac
     3241 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
     3301 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
     3361 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
     3421 cgccaatttg cccacaagga ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
     3481 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct
     3541 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct
     3601 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     3661 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     3721 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     3781 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     3841 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     3901 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     3961 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     4021 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     4081 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt
     4141 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     4201 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     4261 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     4321 gtttatcgct aagagaatca cgcagagcgg cggtatgatc acccgttaag cggcgagcac
     4381 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggag
     4441 tccgggcttt tgtcacaggg gtaaacagta acgttaagag tgccagtgca atagtgcttt
     4501 gtttcacttt
//
LOCUS       DAZFWP020000257         4505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFWP020000257 DAZFWP020000000
VERSION     DAZFWP020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.259"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            204..1361
                     /gene="acrE"
                     /locus_tag="ACHK0Z_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     CDS             204..1361
                     /gene="acrE"
                     /locus_tag="ACHK0Z_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU4504712.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1373..4477
                     /gene="acrF"
                     /locus_tag="ACHK0Z_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     CDS             1373..4477
                     /gene="acrF"
                     /locus_tag="ACHK0Z_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HGU4504713.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1092 a         1101 c         1189 g         1123 t
ORIGIN      
        1 cctctttcaa cccgcgtcaa aataaaacag tagaatatta atcttttttt gtgtttatgt
       61 gccttgagac gccagtattc ataactattc cttacatcga cgaatgataa tttgtaggat
      121 agcaaactgt atttttcttt ctgcgagtta acgcgttgcc tttctgggta aataacgcgc
      181 ttttggtttt ttgaggaata gtaatgacga aacatgccag gtttttcctc ctgccctcct
      241 ttattctgat ctccgcggct ttaatcgccg gttgtaacga taagggagaa gagaaagctc
      301 acgtcggtga accgcaggtt accgttcata ttgtgaaaac ggccccgtta gaagttaaga
      361 ctgaattacc aggccgcacc aatgcttacc gtatagccga agttcgccca caggttagcg
      421 ggatcgtact gaatcgtaat ttcactgaag gcagcgatgt acaagcaggc cagtccctgt
      481 accagatcga tcccgcgacc tatcaggcaa attatgacag cgcgaaaggc gaactggcga
      541 aaagtgaagc cgccgccgcc atcgcgcatt tgacggtaaa acgttacgtt ccgctcgtgg
      601 gtacgaaata catcagccag caggagtacg accaggccat tgctgatgct cgccaggccg
      661 atgccgccgt gattgccgca aaagccacag tcgaaagcgc tcgcatcaat ctcgcttata
      721 ccaaagtgac tgcgccaatt agcggacgta tcggcaaatc gaccgtgacc gaaggcgctc
      781 ttgtcactaa tgggcaaacg actgaactgg cgactgtcca gcagctcgat cctatctacg
      841 ttgatgtgac ccaatccagc aacgatttta tgaggctgaa gcaatccgta gagcaaggaa
      901 atttgcataa ggaaaacgcc accagcaacg tagagttggt catggaaaac ggtcaaacct
      961 atcccctgaa aggtacgctg caattctctg atgtgaccgt tgatgaaagc accggctcca
     1021 taaccctacg tgccgtcttc cctaacccgc aacatacgct tttgccgggt atgtttgtgc
     1081 gcgcacggat tgatgaaggc gtccaacctg acgccattct tatcccgcaa caaggcgtta
     1141 gccgcacacc gcgtggtgat gcaaccgtgc tgattgttaa cgataaaagt caggtggaag
     1201 cgcgccctgt cgttgccagt caggcgattg gcgataaatg gttgattagc gaaggtctga
     1261 aatctggcga tcaagtcatt gtcagcggcc tgcaaaaagc gcgtccggga gagcaggtta
     1321 aagccactac cgataccccc gcagatactg catcgaagta aggtaatctg acatggcaaa
     1381 cttttttatt cgacgaccga tatttgcatg ggtgctggcc attattctga tgatggcggg
     1441 cgcactggcg atcctacaat tacccgtcgc tcagtatcca accattgcac cgcctgcggt
     1501 ttctgtttca gcaaactatc cgggcgctga tgcgcagacc gtgcaggata cggtgacgca
     1561 ggttatcgaa cagaatatga acggtatcga taacctgatg tatatgtcct ccaccagcga
     1621 ttccgccggt agcgtgacaa ttacccttac cttccagtcc gggaccgatc ctgatatcgc
     1681 gcaagtgcag gtgcagaaca aactccagct cgccacgccg ttgctgccgc aggaggttca
     1741 gcagcagggg atcagtgttg aaaagtccag tagcagctat ttgatggtgg cgggctttgt
     1801 ctctgataac ccagacacca cacaggacga tatctcggac tatgtggcct ctaacgttaa
     1861 agatacgctt agccgtctga atggcgtcgg tgacgtacag cttttcggcg cacagtatgc
     1921 gatgcgtatc tggctggacg ccgatctgct aaacaaatat aaactgacac cggttgatgt
     1981 gattaaccag ttgaaggtac agaacgatca gatcgctgcc ggacagttgg gcggaacgcc
     2041 agcgttacca gggcaacagt tgaacgcctc aattattgct cagacgcggt taaaaaatcc
     2101 ggaagaattc ggcaaagtaa ccctgcgcgt aaacagtgac ggctcggtgg tacgcctgaa
     2161 agatgtcgca cgagttgaac ttggcggtga aaactataac gttatcgctc gtatcaacgg
     2221 aaaaccggcg gcgggcctgg ggattaagct ggcaaccggc gcgaatgctc tcgataccgc
     2281 gaaagccatt aaggcaaaac tggcggaatt acagccattc ttcccgcagg gaatgaaggt
     2341 tctctaccct tatgacacca cgccattcgt ccagctttct attcacgaag tggtaaaaac
     2401 gctgtttgaa gccattatgc tggtgttcct ggtgatgtat ctgttcttgc agaatatgcg
     2461 agcaacgctg atccccacca ttgcggtacc cgtggtgtta ttagggacgt ttgccatcct
     2521 cgccgctttt ggttactcca tcaacacact aacgatgttc gggatggtgc ttgccatcgg
     2581 gctgctcgtc gatgatgcga tagtggtggt ggagaacgtc gagcgcgtga tgatggagga
     2641 taagctcccg ccaaaagaag cgacggaaaa atcaatgtcg caaatccagg gcgcactggt
     2701 gggtatcgcg atggtgctgt cagcggtatt tattccgatg gcattcttcg gcggttctac
     2761 tggggcaatt tatcgccagt tctctatcac catcgtttcg gcaatggcgc tttctgttct
     2821 ggtggcattg attcttaccc ctgcgttatg tgcaacgctg cttaaacccg tctctgctga
     2881 gcatcacgaa aataagggcg gtttcttcgg ctggtttaat actaccttcg atcatagcgt
     2941 taaccactac accaacagcg tcggcaaaat cctcggttcc acaggacgat atttactgat
     3001 ctatgcgctg attgttgcag gaatggtggt gttgttttta cgtcttccgt cttccttctt
     3061 acctgaagag gatcagggtg tctttctgac catgattcag ttacccgctg gcgcgacgca
     3121 agagcggacg caaaaagtgt tggatcaagt gacggattac tatctgaaga acgagaaagc
     3181 gaacgttgaa agtgtcttta cggttaacgg ctttagcttc agcggccagg cacaaaatgc
     3241 cggtatggcc ttcgtcagtc tgaaaccgtg ggaagagcgt aatggtgatg aaaacagtgc
     3301 ggaagcggta atccatcgtg ccaaaatgga atttggcaag atccgcgacg gttttgtcat
     3361 tccattcaat atgccagcca ttgttgaact gggcacggca acgggtttcg actttgagtt
     3421 aattgatcag gctgggctgg gtcacgatgc cctaacccag gcccgtaacc agttgcttgg
     3481 tatggcggcg caacatcctg ccagcttagt cagcgtgcgt cctaatggcc tggaagacac
     3541 cgcgcagttt aaactggaag ttgaccagga aaaggcgcag gcattaggtg tttcactttc
     3601 tgacatcaat cagaccattt caacggcgtt gggtgggact tacgttaacg acttcatcga
     3661 ccgtggtcgc gtgaaaaagg tgtatgttca ggcggatgcc aaattccgta tgctgccaga
     3721 agatgtcgat aaactttatg tccgcagcgc caacggcgaa atggtgccat tctcggcctt
     3781 taccacttca cattgggtgt atggctctcc gcgactggaa cgctacaacg gtctgccgtc
     3841 aatggagatt cagggggaag ccgcgccagg aaccagttcc ggcgatgcca tggcgttgat
     3901 ggaaaacctt gcgtcaaaat tacctgctgg cattggttat gactggacgg gtatgtcgta
     3961 tcaggaacgc ttatcgggaa accaggctcc cgctctggta gcaatttcct ttgtggttgt
     4021 tttcctgtgc cttgctgcac tctatgaaag ctggtcaatt cctgtctcgg ttatgttggt
     4081 cgtgccgtta gggattgtcg gcgtgctgct ggcggcgaca ctctttaatc aaaaaaatga
     4141 cgtctacttt atggtgggct tgctaacgac aattggcttg tcggccaaaa acgctatttt
     4201 gattgttgag ttcgctaaag atctcatgga gaaagagggt aaaggtgttg ttgaagcgac
     4261 actaatggca gtacgtatgc gtctgcgtcc tatcctgatg acctctctcg cctttattct
     4321 cggcgtatta ccgctagcta tcagtaacgg tgccggcagt ggcgcgcaga acgcagtggg
     4381 tatcggggta atgggcggaa tggtctctgc aacgttgctg gcaatcttct tcgtaccggt
     4441 gttctttgtg gtgatccgcc gttgctttaa aggataaatc agaaacataa aggcactttc
     4501 gggtg
//
LOCUS       DAZFWP020000258         4503 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFWP020000258 DAZFWP020000000
VERSION     DAZFWP020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.260"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHK0Z_RS24270"
                     /old_locus_tag="pgaptmp_004854"
     CDS             complement(<1..209)
                     /locus_tag="ACHK0Z_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4504714.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHK0Z_RS24275"
                     /old_locus_tag="pgaptmp_004855"
     tRNA            complement(324..399)
                     /locus_tag="ACHK0Z_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHK0Z_RS24280"
                     /old_locus_tag="pgaptmp_004856"
     tRNA            complement(406..480)
                     /locus_tag="ACHK0Z_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHK0Z_RS24285"
                     /old_locus_tag="pgaptmp_004857"
     tRNA            complement(597..681)
                     /locus_tag="ACHK0Z_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHK0Z_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     tRNA            complement(690..765)
                     /locus_tag="ACHK0Z_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHK0Z_RS24295"
                     /old_locus_tag="pgaptmp_004859"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHK0Z_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU4504715.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHK0Z_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHK0Z_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU4504716.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHK0Z_RS24305"
                     /old_locus_tag="pgaptmp_004861"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHK0Z_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU4504717.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4399..>4503)
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     rRNA            complement(4399..>4503)
                     /gene="rrf"
                     /locus_tag="ACHK0Z_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1142 a         1138 c         1016 g         1207 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
     4441 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
     4501 cta
//
LOCUS       DAZFWP020000259         4444 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFWP020000259 DAZFWP020000000
VERSION     DAZFWP020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.261"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            212..>4444
                     /locus_tag="ACHK0Z_RS24315"
                     /old_locus_tag="pgaptmp_004863"
     CDS             212..>4444
                     /locus_tag="ACHK0Z_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HGU4504718.1"
                     /translation="MSRYKTGHKQPLFRYSVLAHCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAK"
BASE COUNT         1161 a         1043 c         1257 g          983 t
ORIGIN      
        1 tacccataaa taaaaatata atcccgcgcc caaccacctg atgagtggct acaggcactg
       61 gatatattag gtggcggtgt actttcttac ataaaggtat ttccttttct gcggaaaagg
      121 aaaccgggaa atctccggtt tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt
      181 tcgttaattc gttcatcagg aaattatctc aatgtcacgt tataaaacag gtcataaaca
      241 accactattt cgttattcag ttctggccca ctgcgtggcg tgggcaaata tctctgttca
      301 ggttcttttt ccactcgctg tcacctttac cccagtaatg gcggcacatg cgcagcatgc
      361 ggttcagcca cggttgagca tggaaaatac cacggtaact gctgataata atgtggagaa
      421 aaatgtcgcg tcgtttgccg caaatgccgg gacattttta agcagtcagc cagatagcga
      481 tgcgacacgt aattttatta ccggaatggc caccgctaaa gctaaccagg aaatacagga
      541 gtggctcggg aaatatggta cagcgcgcgt caaactgaat gtcgataaag atttctcgct
      601 gaaggattct tcgctggaaa tgctttatcc gatttatgat acgccgacaa atatgttgtt
      661 cactcagggg gcaatacatc gtacagacga tcgtactcag tcaaatattg gttttggctg
      721 gcgtcatttt tcgggaaatg actggatggc gggggtgaat acttttatcg atcatgattt
      781 atcccgtagt catacccgca ttggtgttgg tgcggaatac tggcgcgatt atctgaaact
      841 gagcgccaat ggttatattc gggcttctgg ctggaaaaaa tcgccggata ttgaggatta
      901 tcaggaacgc ccggcgaatg gttgggatat ccgcgcagag ggctatttac ctgcctggcc
      961 gcagcttggc gcaagcctga tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg
     1021 taaagataag cgccagaaag acccgcatgc tatttctgcc gaggtgacct atacgccagt
     1081 gcctcttctg acactgagcg ccgggcataa gcagggcaag agcggtgaga atgacactcg
     1141 ctttggcctg gaagttaatt atcggattgg cgaacctctg gagaaacaac tcgatacaga
     1201 cagcattcgc gagcgtcgaa tgctggcagg cagccgctat gacctggttg agcgtaataa
     1261 caacatcgtt cttgagtacc gcaaatctga agtgatccgt attgctctgc ctgagcgtat
     1321 tgaaggtaag ggcggtcaga cactttccct ggggcttgtg gtcagcaaag caactcacgg
     1381 actgaaaaat gtgcagtggg aagcgccgtc attactggct gaaggtggca aaattaccgg
     1441 tcagggtagt cagtggcaag taacgctccc ggcttatcgt ccaggcaaag acaattatta
     1501 tgcgatttct gcggttgcct acgataacaa aggcaatgcc tcaaaacgcg tgcagacaga
     1561 ggtggtcatt accggagcag gtatgagtgc cgatcgcacg gcgttaacgc ttgacggtca
     1621 gagccgtatt caaatgcttg ctaacggtaa tgagcaaaga ccgctggtgc tgtctctgcg
     1681 cgacgccgag ggccagccag tcacgggcat gaaagatcag atcaagactg aactaacttt
     1741 caaaccggct ggaaatattg tgactcgtac cctgaaggcc actaaatcac aggcaaagcc
     1801 aacactgggt gagttcaccg aaactgaagc aggggtgtat cagtctgtct ttactaccgg
     1861 aacgcagtca ggtgaggcaa cgattactgt tagtgttgat ggcatgagca aaaccgtcac
     1921 tgcagaactg cgggccacga tgatggatgt ggcaaactcc accctgagcg ctaacgagcc
     1981 gtcaggtgac gtggttgctg atggtcagca agcctacacg ctgacgctga ctgcggtgga
     2041 tactgatggt aacccggtga cgggagaggc cagccgcttg cgatttgttc cgcaagacac
     2101 taatggtgtc accattggta caatttcgga gataaaacca ggcgtttaca gcgctacggt
     2161 ttcttcgacc cgtgccggaa acgttgttgt gcgtgctttc agcgagcagt atcagctggg
     2221 cacattacaa caaacgctga agtttgttgc cgggccgctt gatgcagcac attcgtccat
     2281 caccctgaat cctgataaac cggtggttgg gggtacagtt acggcaatct ggacggtaaa
     2341 agatgcctat gacaaccctg tgaccagcct cacgccggaa gcgccgtcat tagcgggtgc
     2401 cgctgctgta ggttctacgg catcgggctg gacaaataat ggtgatggga cgtggactgc
     2461 gcagattact ctcggctcta cggcgggtga attagaagtt atgccgaagc taaatggaca
     2521 ggatgcggca gcaaatgcgg caaaagtaac cgtggtggct gatgcgttat cttcaaacca
     2581 gtcgaaagtc tctgtcgcag aagatcacgt aaaagccggc gaaagtacaa ccgtgacgct
     2641 tattgcaaaa gatgcacatg gcaacgctat cagtggtctt tcgttgtcgg caagtttgac
     2701 ggggaccgcc tctgaagggg cgaccgtttc cagttggacc gaaaaaggtg acggttccta
     2761 tgttgctacg ttaactacag gcggaaagac gggcgagctt cgtgtcatgc cgctattcaa
     2821 cggccagcca gcagccaccg aagccgcgca gttgacggtc atcgccggag agatgtcatc
     2881 agcgaactct acgcttgttg cggacaataa ggctccgacc gtcaaaacga cgacggaact
     2941 caccttcacc gtgaaggatg cgtacgggaa cccggtcacc gggatgaagc cagatgcacc
     3001 agtgtttagc ggtgccgcca gcacggggac agagcgacct tcaacaggag actggacaga
     3061 aacaagtaat ggggtctacg tggcgacctt gactctggga tctgccgcgg gccagttgtc
     3121 tgtgatgccg cgagtgaacg gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc
     3181 tggtgacgca tctaaggctg agattcgtga tatgacggtg aaggttgata accagctggc
     3241 taatggacaa tcgactaacc aggtaaccct gaccgttgtg gacacctatg gtaacccgtt
     3301 gcagggacaa aatgtgacgc tgactctgcc gaaaggtgtg accagcaaga cggggaatac
     3361 ggtaacaacc gatgcggcag gtaaagccga cattgagctg atgtcaacgg ttgccgggga
     3421 acacagcatc acggcctcag tgaataatgc tcagaagacg gttacggtga aattcaaggc
     3481 ggatttcagt accggtcagg cgagtctgga ggttgatagc gccgcgccaa aagtggcaaa
     3541 cggcaaagat gcctttacgc tgacggcgac cgttgaggat aaaaatggta accctgttcc
     3601 agggagcctg gtgaccttta atctgccccg gggtgtcaag ccgcttacag gcgataatgt
     3661 ctgggtgaaa gccaacggtg aggggaaagc agagttgcag gtggtttcag tgactgccgg
     3721 aacgtatgag atcacggcat cggcggggaa tagccagcct tcggatacgc agactataac
     3781 gtttgtagcc gataaggcta ccgcaaccgt ctccggtatt gaggtgatgg gcaactatgc
     3841 gctggcggac ggcaaagcca aacaaacgta taaagttacg gtgactgatg ccaataacaa
     3901 tttggtgaaa gatagcgaag tgacgctgac tgccagcccg gcttcgttaa acctggaacc
     3961 gaatggcact gcgacaacga atgagcaagg gcaggctatt ttcaccgcta ccactactgt
     4021 tgcggcgaca tacacactca aggcgcaagt gagtcagacc aacggtcagg tatcaacgaa
     4081 aactgccgaa tctaaattcg ttgcggatga taaaaacgcg gtactcaccg catcatctga
     4141 tatgcaatct ctggtggcgg atgggaaatc gactgcgaag ctggaggtga cactgatgtc
     4201 ggcaaacaac cccgttggcg ggaatatgtg ggtcgacatt cagacgccgg aaggggtgac
     4261 ggaaaaggat tatcagttcc tgccgtcgaa aaatgaccat ttcgtgagcg gaaaaatcac
     4321 gcgtaaattt agtaccagca agcctggtgt ctatacgttc acatttaacg ccctgaccta
     4381 tggcgggtac gaaatgaagc cagtgacggt gaccattacc gcggtggatg ccgatacggc
     4441 aaag
//
LOCUS       DAZFWP020000260         4442 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFWP020000260 DAZFWP020000000
VERSION     DAZFWP020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.262"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..318
                     /locus_tag="ACHK0Z_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     CDS             <1..318
                     /locus_tag="ACHK0Z_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfO"
                     /protein_id="HGU4504719.1"
                     /translation="AAECDGEVVRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRV
                     SRDPFWCRNVLSPSKLREKWDELSLRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAG
                     FRG"
     gene            315..809
                     /locus_tag="ACHK0Z_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     CDS             315..809
                     /locus_tag="ACHK0Z_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4504720.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            809..1462
                     /locus_tag="ACHK0Z_RS24330"
                     /old_locus_tag="pgaptmp_004866"
     CDS             809..1462
                     /locus_tag="ACHK0Z_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4504721.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1459..1785
                     /locus_tag="ACHK0Z_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             1459..1785
                     /locus_tag="ACHK0Z_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4504722.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1782..2171
                     /locus_tag="ACHK0Z_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             1782..2171
                     /locus_tag="ACHK0Z_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4504723.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            2191..3000
                     /locus_tag="ACHK0Z_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             2191..3000
                     /locus_tag="ACHK0Z_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4504724.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3008..3997
                     /locus_tag="ACHK0Z_RS24350"
                     /old_locus_tag="pgaptmp_004870"
     CDS             3008..3997
                     /locus_tag="ACHK0Z_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4504725.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            4015..4398
                     /gene="quuD"
                     /locus_tag="ACHK0Z_RS24355"
                     /old_locus_tag="pgaptmp_004871"
     CDS             4015..4398
                     /gene="quuD"
                     /locus_tag="ACHK0Z_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HGU4504726.1"
                     /translation="MCDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT         1063 a         1048 c         1311 g         1020 t
ORIGIN      
        1 gccgccgaat gtgacggcga ggtggttcgt cccaaagaac cgaactggac agcctgggca
       61 aacgaaattc gcctgatgtg tgtgcaggat ggtcgtactc acaaacaaat ctgcgagatg
      121 tacagccgcg tcagccgcga tccgttctgg tgccgtaacg tgctcagccc gtcgaagttg
      181 cgggaaaaat gggatgagct ttccctgcgc ttatcaccgt ccgtcagcac gtacaccgaa
      241 aaacgcgaag acccgtactt caaatccagt tacgacaacg tggactacag ccagatcccg
      301 gcaggattca gggggtgatc atgagtcttt tgaatgaagt tcagaaattc attgaagccc
      361 atccgggatg tacttccgga gacattgcgg atgcttttgc aggttactca cggcagcgcg
      421 ttctgcagtc agcaagcaag ttacgtcaga gtgggcgtgt ggctcaccgt tgtgaaggag
      481 atacacgcag acatttcccg cgcctgactg agagagcgca ggaaccggaa ccacaaccag
      541 ttcgagaaac cagacctgtg cgcaatttct atgtcggcac taacgatcca cgggtgattt
      601 tgtgcctgac ccgccaggct gaagaactgg agtccagggg cttataccgt cgtgctgcaa
      661 cggtgtggat ggcggcattc cgtgaaagcc actcccagcc agaacgaaac aattttctgg
      721 cgcgtcgtga gcggtgctta cggaaaagca gcaagcgcgc tgcatcgggt gaagagtggt
      781 atctgtcagg gaattacgtg ggggcttaat gagtaataaa tattgccagg cgctggtgga
      841 actgcggaac aaaccagccc atgaactgaa ggaagtgggc gatcagtggc gcacgccgga
      901 caacattttc tggggaatta acaccctgtt tggcccgttt gttctggatc tgtttactga
      961 cggtgataac gccaaatgtg ccgcgtatta cacggcggaa gataacgcgc tggcgcatga
     1021 ctggtcagaa cgtcttgcgg agcttaaagg tgctgccttt ggtaatcccc catacagccg
     1081 cgccagtcag catgaggggc aatacatcac cggcatgcgt tacatcatga aacatgccag
     1141 tgccatgcgt gataagggcg ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt
     1201 gtggtggccg gaagatgcgg accatattgc ttttattcgc gggcgtattg gttttgaact
     1261 gcctgcctgg tttatcccga aggatgagaa gcaggtgccg acaggcgctt tcttcgctgg
     1321 tgctattgct gttttcgaca agacctggaa gggaccggca atcagctaca tcgggcgcga
     1381 tgaacttgag gcatgtggtg aggcctttct ggcgcaggtt cgccagcagg cggaaaaact
     1441 ggtcagggag atggcggcat gacgacgtta actcaatgcc agcagcaggt gctggatatg
     1501 ctgatttctt accagcaaga acgtggcttt ccgccaacca atcaggaggt ggcaaccatg
     1561 ctgggatacc gttcggtgaa tgcagcggtg gagcatcttc gcgcactgga gaaaaaaggc
     1621 gtcatcacga taaagcgtgg cgtggcccgg gggataactc ttcataccgc ggtgaaggac
     1681 gacgacagcg aggcggtcgg gattatccgc gcactgcttg ccggtgagga aaacgccagg
     1741 ctgcgtgcag cccactggtt acatgagagg ggcctgaaag tatgaagctg atcttgcctt
     1801 tccctcccag cgtgaacacg tactggcgac accccaacaa aggggcgttt gctggtaaga
     1861 gtctgataag cgcagcgggg cgcaaattcc agagcgcggc gtgcgcagca atagttgagc
     1921 agttacgtcg tctgccgaaa ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc
     1981 ctccggataa ccggatccgc gatctggaca actataacaa ggcgctgttt gacgccctga
     2041 cccatgcggg tgtgtgggaa gacgacagtc aggtgaaaag aatgctggtg gagtggggac
     2101 cggttatccc gaaagggaag gtcgagatca ctatcagtaa gtacgagaaa acggcgggtg
     2161 cagccgcctg atcaagagga gaaacgaagt atgaataatc tgatggtcat tgatggtatt
     2221 gaagttcgtc gtgatgctta tgggcgttac agcctgaacg atctgcatcg cgcagcagta
     2281 gcatctggtg caaatgccag aaccaaggag ccgggaaagt ttctttccag ccaacaaact
     2341 gttgagcttg ttcatgaatt gaccaacacc cagaatttgg gtgttgaccc ggtgagtgtg
     2401 attcatgggg gaaatgaacg gggaacgtat gtctgcaagg aactggtgta tgcctatgca
     2461 atgtggatca gcccgtcatt ccatctgaag gtgatccgta ctttcgatat ggtaaccagc
     2521 gcaccggaaa aattatccgg acaggctgct gacaagatgc aggctggcgt gatcctgctg
     2581 gactttatgc gccgggagtt aaacctgtct aactcttcag tgcttggtgc ctgtcagaaa
     2641 ctccaggagg ctgttggctt accgaatctg gcaccgcgct atgccattga tgctcctgct
     2701 gatgcacacg atggctcaag tcgcccgaca ctgtcactga gtgcactgct gaaacagtat
     2761 ggtatacgcc tgacggctaa tcaggcatat caccagatgg tgaaactggg gatcgtcgag
     2821 cagcgcgaac gatacagccg tacagcgatt aacaacatca aaaaattctg gtcgctgaca
     2881 gcgaaaggtt gcatgttcgg caagaacatc accagtcccg caaatccgcg cgagacgcag
     2941 ccgcatttct tcgaatcccg attccctgag ctgttaaagc tgctcgatac cgttcattga
     3001 ggtgaccgtg agagcactac tgacccctga aattgccccg cgtatgggga tcgtattgtt
     3061 caggccaggt tcagagctga tgcccctgtt tatgcagggg cgtgtcctgc tggagcctga
     3121 gccggagcgt tattcatctt tcgccagtgg tgccgttccg gcggcatcac aaccgctggc
     3181 ggatgatcct gccgttcggg ccgtgttccg caatgaggca gtgatccgtc gtgctggtgg
     3241 cgtggaatgt cttgaaagct ggttacttcg tgaaaaaggc tgccagtggc ctcattccga
     3301 ctggcacagc gagaacatga ccacaatgcg acacgctccg ggtgcaatcc gtctgtgctg
     3361 gcactgcgat aaccagctgc gcgatcagtt cacggaacgg ctggaatcaa tggcaacgga
     3421 taactgtgcc cgctgggtgt tgtctgttgt gcgtcgggat ctcggttttg atgacagtca
     3481 cgttgtgaca atgccggaac tgtgctggtg gctgattcgt aatgatctgg cggatgcctt
     3541 accggaaagt gcagcccgta aggcactgag attaccgaag cctgttgtgc cgtctgttac
     3601 ccgggaaagt gaccttgtgc cttcggttcc tgccaccagc atcatccagg ataaggcgaa
     3661 aaaggtgctg gcgctgaaag tggatccgga gtcgccggag tcttttatgt tacgcccaaa
     3721 acgtcgccgc tgggttaatg aaaagtacac gcgctgggtt aagacacagc cgtgtgcatg
     3781 ttgtggaaag ccagctgatg atccgcatca tctgataggc catggtcagg gtggaatggg
     3841 tacaaaagcg catgaccttt ttgtgctgcc tttgtgcaga aaacaccacg acgaactgca
     3901 tgcggatacc gtggcatttg aagagaagta tggctcccag ctggagctga tatttcgttt
     3961 tatcgatcgc gcactggcga ttggtgtgct ggcctgattt tgtggagaaa gttaatgtgt
     4021 gatattcaga tggttcttga acgttgggga gcgtgggcgg ctaataatca tgaagatgtg
     4081 acctggtcgt ccattgccgc cggttttaag ggattaattc cttcaaaagt aaaatctcgc
     4141 ccgcaatgtt gtgacgatga cgcgatgatc atttgcgggt gcatggcccg tctgaaaaag
     4201 aacaacagcg atttgcacga tttattagta gattattatg tagtcggtat gacattcatg
     4261 tcactggcag gtaagcattg ctgctctgat ggttatatcg ggaaaaggtt acagaaggct
     4321 gagggcataa ttgaagggat gttaatggca ttagatatcc ggttagagat ggatatcgtt
     4381 gttaataact ctaattaata tgccaattgt ttactgaaag ttattaaaaa tggggcgttg
     4441 ca
//
LOCUS       DAZFWP020000261         4398 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFWP020000261 DAZFWP020000000
VERSION     DAZFWP020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.263"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..401
                     /gene="cesL"
                     /locus_tag="ACHK0Z_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             48..401
                     /gene="cesL"
                     /locus_tag="ACHK0Z_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HGU4504727.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTF
                     QQHVQIIERVRRMTS"
     gene            398..2425
                     /gene="escV"
                     /locus_tag="ACHK0Z_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     CDS             398..2425
                     /gene="escV"
                     /locus_tag="ACHK0Z_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGU4504728.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKKTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            2409..3749
                     /gene="escN"
                     /locus_tag="ACHK0Z_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             2409..3749
                     /gene="escN"
                     /locus_tag="ACHK0Z_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGU4504729.1"
                     /translation="MISEHDFVLERYPRIQRVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYRIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGDEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYADPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            3809..4129
                     /locus_tag="ACHK0Z_RS24375"
                     /old_locus_tag="pgaptmp_004875"
     CDS             3809..4129
                     /locus_tag="ACHK0Z_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGU4504730.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            4122..>4398
                     /locus_tag="ACHK0Z_RS24380"
                     /old_locus_tag="pgaptmp_004876"
     CDS             4122..>4398
                     /locus_tag="ACHK0Z_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HGU4504731.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNG"
BASE COUNT         1244 a          783 c         1006 g         1365 t
ORIGIN      
        1 ttttcaagca ataatatcca cacatatact tacttcagag cctgcatatg aatcttttaa
       61 ttaaaagaaa cgtagaagag tttttaaggt tattagggaa tgatttttat ttgtttgata
      121 atcgcgtgga aattgatttt aatggttttt ctttttttat tgaaataatt gatagtaatg
      181 tgtttattac gtttgcctta gagtataacg aaaatgcgtt tttttctttt ttcaatgccc
      241 ttgcccctga acggacgcag ggagtgattg aacatatttt tgtctacgat aacaaattgt
      301 gcttaagttg tttgttaacc aatattgatg tgttttctct aatgaatact tttcagcagc
      361 atgtgcagat tattgaacga gttcgcagga tgacatcatg aataaactct taaatatatt
      421 taaaaaagca gagtcatatc acgatcttat tctggctctc ttcttcttta tggctgtaat
      481 gatgatgatt attccattgc caacagttgt ggtggatatc attatcgcga ttaatatatc
      541 gacagcattg cttttattaa tgctctcaat ctatataaaa aatcctcttg agctaacttc
      601 ttttcctaca atcctgttga ttacgacgtt gatgcgcctg tcgctcagtg ttagtacaac
      661 cagacttatt ctgctgcatc acgatgcggg tgacattatt tactccttcg gtaactttgt
      721 tgttggcggt aatattgttg ttggcctggt catttttacc attatcacca ttgtacaatt
      781 tatggttatt acaaaagggg ctgagcgtgt tgctgaagtg agtgctcgtt tttctcttga
      841 tggtatgcca ggaaaacaaa tgagtattga tggtgatatg cgtgcaggcg ttattgaccc
      901 gcttgaggca aaagttcttc gctcccgagt gcaaaaggaa agccagtttt atggttcaat
      961 ggacggggcg atgaagtttg taaaagggga cgctattgcc ggtatcatca ttgttttagt
     1021 taacctgttt ggtggcgtgc tcattggaat gtggcaattt gacatgccat ttagtgaagc
     1081 acttagcctg ttttctgtat tgtctgtcgg tgatgctttg gttgcccaga tccctgcgct
     1141 tattatttcc gtcactgcgg gggttgttgt tactcgtgtt cccggtgaaa gcgaaaaaga
     1201 agaaaacctt gcaggtgata ttgttcaaca ggtttctgtt aatagtcgcc cttttttaat
     1261 cagtgccgca ctcatgatcg taatggcgat tattcctggc tttcctacat tggtcttctt
     1321 gtttctggcg gtttgcctgt tgggtatcgc ctggaaatta caaaagaaaa agacgtttgg
     1381 aaccggaaat aataaggacg ctatgggagc tgatttgtct aatagtcaaa atatctcacc
     1441 gggtgccgag ccgttgattt taaacttaag tagtaatatt tatagctcag atattacaca
     1501 gcagattgag gttatgcgtt ggaatttctt tgaggaaagt ggaattccat tgcctaagat
     1561 tattgttaat ccagttaaaa acaatgatag cgctatagaa tttttgctct atcaagagtc
     1621 aatatacaaa gatactctga cagatgatac tgtttatttt gaggctgggc atgcagagat
     1681 atcatttgaa tttgtccagg aaaagctttc ggctaactct atcgtgtata aaacgaatga
     1741 ggctaaccaa caactagctc acgtctcagg tatggatgtt tatgcaaaaa caaatgataa
     1801 gataacgttt ttgctaaaga aacttgtatt gtctaatgcc aaagagttca tcggcgtaca
     1861 agaaacgcgt tatctgatgg acatcatgga gaggaaatat aacgaacttg tgaaagagct
     1921 tcagcgtcag cttggtctga gcaagattgt ggatattctt cagcgtctcg tagaggaaaa
     1981 tatctcaatt agagatctac gaactatttt tgaaacgctt attttttggt ccacaaaaga
     2041 aaaagatgtg gttatcttgt gcgaatacgt tcgtatcgcc ctacgtcggc atattttagg
     2101 tcgctatagc gttcgcggta cacttctgaa tgtttggctt attggctctg atattgaaaa
     2161 tgagctacga gagtcgatca gacaaacgtc atcaggttcg tatttgaata tctcaccgga
     2221 gcgaagtgag cagattattg gcttcttaaa aaatattgtg aatccaacgg ggaacggcgt
     2281 cattctgacc gctttagata tcaggcgcta cgtgaagaaa atgattgaag gttcgttccc
     2341 gtcagtcccc gtgctctctt ttcaggaagt tgggaataat atcgaactta aagtattagg
     2401 aacggtaaat gatttcagag catgattttg tattggaaag atacccacgt atccagagag
     2461 tgctcaatag cactgtgccg acattatcat taaattcgtc taccagatat gaaggcaaga
     2521 ttaccaatat cggcgggacg attatcaagg cgcgtttacc aaaagcgcgt attggcgcct
     2581 tttacaggat agaacccagt caacgtttag ctgaggtgat cgctattgat gaagatgagg
     2641 ttttcctgct gccttttgag catatttccg gcatgtattg tggccagtgg ctaagttatc
     2701 agggggatga gttcaagatt cgtgttgggg acgaactcct tgggcgactg gttgatggta
     2761 taggcagacc aatggggagc aatattactg caccttatct tccgtttgaa cgcagtttgt
     2821 atgctgaccc ccccgatcct ttattaaggc aggttattga tcagccattt acgcttggcg
     2881 tcagggcaat agatggttta cttacttgcg gtattgggca acgtatcggt attttcgcgg
     2941 ggtccggcgt tggtaaaagt acgcttttgg gaatgatttg taatggcgca tccgcggata
     3001 ttattgtcct tgcccttatc ggcgagcgtg gtcgcgaagt aaatgaattc ctcgcgcttt
     3061 tacctcaatc cacgctttct aaatgtgtac tggtcgtcac aacgtcagac cgtcccgcgc
     3121 tggaaagaat gaaggccgca tttacggcga caaccattgc agagttcttc cgggatcagg
     3181 gcaaaaatgt attattaatg atggattccg taactcgtta tgcccgagcc gcgcgtgatg
     3241 ttgggcttgc atcgggagag cctgatgtaa gggggggatt ccctccgagt gttttttcct
     3301 cgttacccaa attattagag cgtgcggggc ctgcgccaaa aggctcaata accgcgattt
     3361 atacggtgtt gttagaaagc gataatgtta atgatcccat tggtgatgaa gtccgctcta
     3421 ttcttgatgg gcacattgtg cttacccgag aacttgcaga ggaaaatcat tttcctgcaa
     3481 ttgatattgg tttaagcgcc agtcgtgtta tgcataatgt tgttacatcg gagcatttgc
     3541 gtgcggcggc agaatgcaaa aagcttatcg caacctataa aaatattgag ctgcttattc
     3601 gtattggtga gtacacgatg gggcaagatc ctgaagcgga taaggcaata aaaaacagga
     3661 aagcaattca gagctttata caacaaagca ccaaagatat cagtagttac gaaaaaacga
     3721 ttgaaagcct attcaaagtg gttgcctgaa tatgttggac agaattttat ctattcgtaa
     3781 aagcagagcg aatcgattga gagaatcaat ggctaagatt aactctcaga taaaagaggt
     3841 ggatggtaaa ctcgatgact gcgagcaagc cattaaagag tcgattgcct ctaagcaggc
     3901 gtattgtgca tcgctggtga atctggataa agtgtcgttg tataaatacc agattaaaaa
     3961 caatgcattt gatgagcaaa agcagcgatt atatgagaaa aaatcctcac tttctaaaga
     4021 aaagcgttca ttgctcgatt cacaaaaacg aacaaaagag gatctacaac atgttaataa
     4081 atcaatagag aagttatctt ttgccataaa ggaacattac tttgactagc atttctctaa
     4141 aaagaaattt gatagagcct gtttcttttc agccgtcaga tgattggccg cagggagaag
     4201 aaagttttaa tgatattttt aatcgcataa aaaaaagaac acctgacact tcgttcatac
     4261 gaatggcttc tttatggaaa cgattgttct acgctcctgg acagaggcga cgctactttg
     4321 aacaaggaga gcgttcattt agtattattt gtggaaagtt acgcggaatt gttttaacaa
     4381 taaaatgtag taatggaa
//
LOCUS       DAZFWP020000262         4390 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFWP020000262 DAZFWP020000000
VERSION     DAZFWP020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.264"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..461
                     /locus_tag="ACHK0Z_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             <1..461
                     /locus_tag="ACHK0Z_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4504732.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRS
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            832..3938
                     /gene="agn43"
                     /locus_tag="ACHK0Z_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /gene_synonym="flu"
                     /pseudo
     CDS             832..3938
                     /gene="agn43"
                     /locus_tag="ACHK0Z_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
     gene            4059..>4390
                     /locus_tag="ACHK0Z_RS24395"
                     /old_locus_tag="pgaptmp_004879"
     CDS             4059..>4390
                     /locus_tag="ACHK0Z_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504733.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAV"
BASE COUNT         1006 a         1197 c         1293 g          894 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggtctca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg tgccgtccgt gactcggtgg
      421 tctctgttgc ccgcgctgta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgacccc ccccctcata cccacctctt
      541 tctcttgcgt gacttccctg ctgttaatag aataaaacga tcgataaaac aggtatcgat
      601 cgttactatc gatcgtttat agtggttaat ggtctgcgaa aaggcagctg aatctcttca
      661 tcatgcagaa cggaattgca cacaacggac tgatgcttct ctggcggatg agagagggga
      721 aacatttatg tccgggaaaa accactttat ctgaccatgc tgttttgtac ctgccggtac
      781 ccacatctgt gggtaccggc ttttttattc accctctgta aggaaaagct gatgaaacga
      841 catctgaaca ccagctacag gctggtatgg aatcacatta cgggcaccct ggtggtggcc
      901 tccgaactgg cccgctcacg gggaaaacgc gccggtgtgg cggttgcgct gtctcttgct
      961 gctgtcacat cagtcccggc actggctgct gacacggttg tacaggcggg agaaaccgtg
     1021 aacggcggaa cactggcaaa tcatgacaac cagattgtct tcggtacgac caacggaatg
     1081 accatcagta ccgggctgga gtatgggcca gataacgagg ccaataccgg cgggcaatgg
     1141 gtacaggatg gcggaacagc caacaaaacg actgtcacca gtggtggtct tcagagagtg
     1201 aaccccggtg gaagtgtctc agacacggtt atcagtgccg gaggcggaca gagccttcag
     1261 ggacgggcag tgaacaccac gctgaatggt ggcgaacagt ggatgcatga gggggcgata
     1321 gccacaggaa ccgtcattaa tgataagggc tggcaggtcg tcaagcccgg tacagtggca
     1381 acggataccg ttgttaatac cggggcggaa gggggaccgg atgcagaaaa cggtgatacc
     1441 gggcagtttg ttcgcgggga tgccgtacgc acaaccatca ataaaaacgg tcgccagatt
     1501 gtgagaactg aaggaacgac aaataccact gtggtttatg ccggcggcga ccagactgta
     1561 catggtcacg cactggatac cacgctgaat gggggatacc agtatgtgca caacggcggt
     1621 acagcgtctg acactgttgt gaacagtgac ggctggcaga ttgtcaaaaa cgggggtgtg
     1681 gccgggaata ccaccgttaa tcagaagggc agactgcagg tggacgccgg tggtacagcc
     1741 acgaatgtca ccctgaagca gggcggcgca ctggttacca gtacggctgc aaccgttacc
     1801 ggcataaacc gccttggagc attctcggtt gtggagggta aagctgataa tgtcgtactg
     1861 gaaaatggcg gacgcctgga tgtgctgacc ggacacacag ccactaatac ccgcgtggat
     1921 gatggcggaa cgctggatgt ccgcaacggt ggtaccgcca ccaccgtatc catgggaaat
     1981 ggcggtgtac tgctggccga ttccggtgcc gctgtcagtg gtacccggag cgacggaaag
     2041 gcattcagta tcggaggcgg tcaggcggat gccctgatgc tggaaaaagg cagttcattc
     2101 acgctgaacg ccggtgatat ggccacggat accacggtaa atggcggact gttcaccgcc
     2161 aggggcggca cactggcggg caccaccacg ctgaataacg gcgccatact taccctttcc
     2221 gggaagacgg tgaacaacga taccctgacc atccgtgaag gcgatgcact cctgcaggga
     2281 ggctctctca ccggtaacgg cagcgtggaa aaatcaggaa gtggcacact cactgtcagc
     2341 aacaccacac tcacccagaa agccgtcaac ctgaatgaag gcacgctgac gctgaacgac
     2401 agtaccgtca ccacggatgt cattgctcag cgcggtacag ccctgaagct gaccggcagc
     2461 actgtgctga acggtgccat tgaccccacg aatgtcactc tcgcctctgg tgccacctgg
     2521 aatattcccg ataacgccac ggtgcagtcg gtggtggatg acctcagcca tgccggacag
     2581 attcatttca cctccacccg cacagggaag ttcgtaccgg caaccctgaa agtgaaaaac
     2641 ctgaacggac agaatggcac catcagcctg cgtgtacgcc cggatatggc acagaacaat
     2701 gctgacagac tggtcattga cggtggcagg gcaaccggaa aaaccatcct gaacctggtg
     2761 aacgccggca acagtgcgtc ggggctggcg accagcggta agggtattca ggtggtggaa
     2821 gccattaacg gtgccaccac ggaggaaggg gcctttgtcc aggggaataa gctgcaggcc
     2881 ggtgccttta actactccct caaccgggac agtgatgaga gctggtatct gcgcagtgaa
     2941 aatgcttatc gtgcagaagt ccccctgtat gcctccatgc tgacacaggc aatggactat
     3001 gaccggattc tggcaggctc ccgcagccat cagaccggtg taaacggtga aaataacagc
     3061 gtccgtctca gcattcaggg cggtcatctc ggtcacgata acaacggcgg tattgcccgt
     3121 ggagccacgc cggaaagcag cggcagctat ggcttcgtcc gtctggaggg tgacctgctc
     3181 agaacagagg ttgccggtat gtctgtgacc gcgggggtat atggtgctgc tggccattct
     3241 tccgttgatg ttaaggatga tgacggctcc cgtgccggca cggtccggga tgatgccggc
     3301 agcctgggcg gatacctgaa tctgatacac aacgcctccg gcctgtgggc tgacattgtg
     3361 gcccagggaa cccgccacag catgaaagcc tcatcggaca ataacgactt ccgcgcccgg
     3421 ggctggggct ggctgggttc gctggaaacc ggtctgccct tcagtatcac tgacaatctg
     3481 atgctggagc cgcaactgca gtacacctgg cagggactct ccctggatga cggccaggat
     3541 aacgccggtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggcttccgt
     3601 ctgggcagcc acaacgatat gaactttggt aaaggcacct catcccgtga caccctgcgc
     3661 ggcagtgcca aacacagtgt gcgtgaactg ccggtgaact ggtgggtaca gccttctgtt
     3721 atccgcacct tcagctcccg gggggacatg agcatgggta cagccgcagc cggcagtaac
     3781 atgacgttct caccgtcaca gaatggtacg tcactggact ggaagcccgt gtccgggaaa
     3841 atatcaccct gggcgttcag gcaggttatg tccacagcgt cagcggcagc agcgctgaag
     3901 gctataacgg tcaggccacg ctgaatgtga ccttctgaca gaaccatcgc ctctctgtgg
     3961 tcccggtcat catgatcggg acccggaccg gcgcaacgga tattcaacgc cacattcgct
     4021 ggcattaaca ataacatgat attcatcacg gagtgactat gttacagata gtcggcgcgc
     4081 tgatcctgct gatcgcagga tttgccattc ttcgcctttt gttcagagca ttaatcagca
     4141 cggcttctgc gctggcaggg ctcatattgc tgtgtctgtt cggcccggcc ttactggctg
     4201 gctatatcac cgaacgcata acccggttgt tccatattcg ctggctggca ggcgtatttc
     4261 tgacgattgc cggaatgatc atcagcttca tgtggggact tgatggtaaa catatcgcgc
     4321 tggaggctca cacctttgac tctgtgaaat ttattctgac caccgctctc gccggtggtc
     4381 tgctggctgt
//
LOCUS       DAZFWP020000263         4349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFWP020000263 DAZFWP020000000
VERSION     DAZFWP020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.265"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            367..1851
                     /locus_tag="ACHK0Z_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             367..1851
                     /locus_tag="ACHK0Z_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4504734.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2173..2775)
                     /locus_tag="ACHK0Z_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             complement(2173..2775)
                     /locus_tag="ACHK0Z_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504735.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2862..3068)
                     /locus_tag="ACHK0Z_RS24410"
                     /old_locus_tag="pgaptmp_004882"
     CDS             complement(2862..3068)
                     /locus_tag="ACHK0Z_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4504736.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3827..3976
                     /locus_tag="ACHK0Z_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             3827..3976
                     /locus_tag="ACHK0Z_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HGU4504737.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT         1144 a          923 c         1140 g         1142 t
ORIGIN      
        1 gcattcagtg caggaactgt agtcttcttt attttattct tcttcaggag ggggggtgat
       61 tcctgaaggc gttattcccg gtgttctgtt tatgattctt cccggcagga aaccggtatc
      121 tgcaataccc gggatgatgc tttttgttcg tacgtggttg tgtaaaattt gaatagtgga
      181 tgtgctccga agggaaggag gggacacaca gattcatttt gtctttcttc agcagcaaaa
      241 tacactgtgg ttattattta accattaaat caacaaaaat ttttacctca tattgatttg
      301 actgctattt ttgctattaa tagccagaat tgcagtgctt ttatatctgt gtgtagtgag
      361 gttactatgt cattcggtca gccttgtgat gagtttcccc tgagttcgct gcctccactc
      421 attcgtgatg ccgttattga agctcagcaa attacgcagg ctccccttgg gcttgtggca
      481 gcgtctgccc tgggcgctgt ttcacttgtc tgccagaatc tgattgatgt ctgcagactg
      541 aatacactgc gggggccggt ctctcttttt ttcctgacgc ttgccgagtc cggagaacga
      601 aaaaccgcag tggacaaatt attaatgaag cccctgtacc agcaggagat gcagttatat
      661 tccagataca aaagtgaact ggctgtctgg aaaaacaagg aagagctttt aaaggctcag
      721 aaaaaagcat tgttgtcaaa actgaataaa gaactccgca aaggcgcgga tgagtcagaa
      781 accctcagac aactggaagt acttcagaaa aacagtgcgg aagaacctgt gaggtataaa
      841 ttcattttta atgatgccac gactgcggca attaagaatc agctttgtgg taaatggcgt
      901 tcagtgggta ttatgtcaga tgaagccggt attatatttg atggatatac cctgtctgag
      961 ttgcctttta ttaacaaaat gtgggatggc tctgtattgt ctgtggacag aaagaatgaa
     1021 cccgaacaga tgattgaaaa tgccagaatg acgttatccc tcatggtgca acccggtctt
     1081 tttgaccgat atatggagcg aaaaggttct gttgccaggg acagtggttt tcttgcccgt
     1141 tgtcttatca gtaaacctgc aaccacgcag gggaaacgct ttattaacgg agctgtgatt
     1201 ccgggaggat cattaacggc atttcatgaa cgattaatgg aactggccag aggcagtatt
     1261 gaaaaatcca gtgaggatga acggtattgt ctgcattttt cgcctgaagc gcaaaaaata
     1321 tttattgagc actataatgt tctggagcag gatttatccc cttccggacc cttaagcccg
     1381 tttcggggac atgtttcaaa aaaaacagaa aacattgcaa gaattgcagc gttatttcag
     1441 tatttcagct atggtgaagg gaaaatctct gccgatatca tgacgtctgc tgtcgtaata
     1501 agttcgtggt acacagatga atataaaaaa ttatttgcgt tacctgatga atctgaactg
     1561 cagcagaagg acgccgagga gctgttcgac tggctcattg aggagtgccg cggagaatgc
     1621 ccgccccgtg tcaggaaaaa ttatattctc cagtgtggcc cgggccggtt cagaaacagg
     1681 aaaaaactga acgcattgct gaacattctt gaatctcagt tcaggctttc tgtcgtgccg
     1741 gaaggaaaaa ccatgtatgt tctgcttccc cagatagcca gtttaaagtt gtctgatgtc
     1801 tctggtattt ttacctcggg ttatcactac aataagctga gagcgaagta accggtgagc
     1861 agtttttctc cattatgctt cagacttaga tgattgatta ttatggggat ttcatcaccg
     1921 atattcagat gtatgaatat cggtgatgaa ctaaagctca ggagctgcgt ggtggtgatg
     1981 aatcaacagg catataactg atacccgccg gtcagaagtt ccagcctgcg actttcctgt
     2041 ctgcgtgccc gcagtccggc gttcaggtgc agcgtgtgac agaacgcacg gagagcgaaa
     2101 cggcaggcag acaggccgtt cagtcagccc tggcaccctg cccggatacc agcttccctg
     2161 aaacaactgg catcaggtgc gcttatcgtc aaccgttgtg cggtatgcca ggagcacgta
     2221 ctgatccatc agatccgtca gtgctgcgct ctgcttctga cggttgatga tccattcctc
     2281 cagctctttt tctgatttcc gtttcagatt ctccgtccag tcgtcaaggc agtttccggt
     2341 cattaaatca agccagcaaa gccagaccag tttgaggcgc aactccgtgc caccaaactg
     2401 acggaagagg ctgatcagtt tatttgccag cgaacgctgc ctcagacgtt cacattcaga
     2461 caggagaatg ccttccagca tcggatgatg aaccagcaga tgttcactgg tgcataaatt
     2521 cactatcaga tcatcatcca ggggcggcgt ggtccgtttg tccggaccat aaatcagccg
     2581 gtgcagactc tggggtacga tggcaatctc cggtttttca ccattgaatg acacccaccc
     2641 gggcgtcaga ttatggaagt agtagatatt cggatgacac acgccccagg atacggcgtg
     2701 ccgggtgaag ccggtcagcc atttctggta gctttcgtgg agcagctcgt aacgggtctg
     2761 atactgtggt ttcattttca tattcctcac gggggataac aggcagcaga acgccgccgg
     2821 caggccggag actgcgtctg ctgcagatga acggttaaga attacggacg ggactgtgca
     2881 atacgcgcct gcagccaggc ttccacttcg cttttcagcc atcgggagct gcggcccatt
     2941 ttgatgggcg caggaaagtc accatccttg atgagtttgt aaaaccactt atcggtcagg
     3001 ccggtcagtt gagtgataaa tgccatgtcg accatctggt catccatcag tgaaactggg
     3061 gtgatcatga gaatgttcct ccggatgttg ttaaaccagc cggtgtggtc atgaaaacaa
     3121 cgcaccacct tcaccggcat aacaaccggg gagaaacgta aaaaataatc gggaatgtga
     3181 ccgccaggag ataaacgggg aggttacggt ggctgagccg taccggtatg ccgggtggtt
     3241 gttacgaaac gatgcttccg cacgcgtgcg cgataaagaa gggatgattt tcgtgaggaa
     3301 gaggggaaaa ctggcgttct gataattgca gagttattta cggggcggtt acgatggggc
     3361 agaccgtgtc cggtgggcag gcgacaggta tcaccgatgg gataagactg tctgattggt
     3421 attataaata tataagagag atcaaacaca tgagatcaaa tacatgagtt catatatgtg
     3481 aaataaacac ggtgagtgtg atcagtgggc atgatgagaa cgcagtagca acagactgcg
     3541 ctatccctga tagctggcgt ataacccgcg tatataaaac gtatctgcaa tgtacgtgaa
     3601 cagagtgcat taagcatccg tctgagcggt aaacatatcc gacctggtga gtgacagtct
     3661 gagagaccgg ttaagtggtg gctcgggagt aatgagtttg gggtgggtac cagaatgcca
     3721 gtctggcggt atgctgacag tatcacgtta attgcagcat aaagtgatga gaggctaatg
     3781 gaagttaaaa ccaaagcagt tccgtcgttg ttcatcccgg gagacgctgg aaaaagtgat
     3841 ttcccacacg cgttataaac ttacctctgc ggaactggaa gccttcaact ctgtggttga
     3901 tcactggctg gcagaactga caatgaacaa gctttacgat cgtgttcccg cttcagtctg
     3961 gaaatatgtc atctgagcgt tcggtaatca caactgcatc gcgccaggtg ttatccgctt
     4021 tgtgattctt attggctctt ctcaggttat taaaagctga atatgttcct gttgtcggga
     4081 accaggataa taccgggaac gttaagcggt atagcgacaa caaagaattc gaaatcaaaa
     4141 gggaatggtg ccggaggcgt gggcggtaat gggctgattt tcaggtatta ccggcttaca
     4201 gcgtgtggtt gatattgagg ggctgtgagt ttcacgtatt tgtcagaaac tcagacgagt
     4261 tttcctgtat gggacgaaat aaggcagcgg caatacatgg aaaaatttaa tatggtatga
     4321 aggagaacct gtcggttacg cttaatata
//
LOCUS       DAZFWP020000264         4336 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFWP020000264 DAZFWP020000000
VERSION     DAZFWP020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.266"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(40..825)
                     /gene="ygcW"
                     /locus_tag="ACHK0Z_RS24420"
                     /old_locus_tag="pgaptmp_004884"
     CDS             complement(40..825)
                     /gene="ygcW"
                     /locus_tag="ACHK0Z_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4504738.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            1144..2421
                     /gene="yqcE"
                     /locus_tag="ACHK0Z_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     CDS             1144..2421
                     /gene="yqcE"
                     /locus_tag="ACHK0Z_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU4504739.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2448..3926
                     /gene="ygcE"
                     /locus_tag="ACHK0Z_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     CDS             2448..3926
                     /gene="ygcE"
                     /locus_tag="ACHK0Z_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU4504740.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         1168 a          976 c         1022 g         1170 t
ORIGIN      
        1 taatcattaa taagagaaaa cagaaaccac cgttatggat tagcgcacta aataaccacc
       61 atcgaccact aataaatgcc cattgacata atttgaagcc ggacttgcga ggaatacagc
      121 tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat caagaacgcg
      181 ctgattggtt tctggattac tgcgtgtcgc cagcgtaata tctgttgcat aatagccagg
      241 ggcgatacca tttacctgaa tattatattg acctagctca tcacaataag ctttggtgaa
      301 cccggcaaga gcatgtttag tggcagaata tgcaggtgac cattgtccac ctaagtaaga
      361 gaacaatgaa cagatattaa taattttgcc gcttttctgc gggatcataa tttttgcagc
      421 ttcatagctt aactcgaatg cggcggtcag gttcacatca atcatcggat cccagtcggc
      481 acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttta ccagaatatc
      541 aactgtaccg aaacgctcac agcaagcagc gataatcttc tgcggcgcgc cttctgcggt
      601 gatatccacc tgcatgaagt ccacctcaac accctgtttt tcaatcattt cctttgtttc
      661 gccgttatct ttgacgaaac taggaataaa gatatttgcg ccagctttgg ccaacgccat
      721 ggcaaatgcc tggcctaaac cgctattccc accggtaaca attgcggttt tacctttcag
      781 tgagaaaaaa tccattgaga acgcattgag agattcgatt gacatagtta gctccaattt
      841 ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag agggaagtta cctttctcat
      901 tatctctggt aactggagat aatttttagc ataacggcat aactgctaat gtgatcgtaa
      961 tcacagtgtg atattcgtat tgtcaaatag ttttaaaaag cagaagcgtg attactctca
     1021 cacaacacct ctgctttcca tgttatttct tattgcaatt actagagacc tgagaaaagt
     1081 aatccacctg aaagggcggc ttacttttct cttttttttg tctattacaa atgggaatac
     1141 gatatgcaac acaactcata tcgccgttgg ataaccctcg cgataattag ttttagcggc
     1201 ggcgttagtt tcgacctggc ttatttacgt tatatttatc aaattcccat ggcgaaattt
     1261 atgggattca gcaataccga gataggttta ataatgagta cctttggtat tgcggctatt
     1321 attctttatg cccccagcgg cgttattgcc gataaatttt cacaccgcaa aatgattact
     1381 tccgcgatga tcattaccgg attactgggt ctgttaatgg caacgtatcc accgctgtgg
     1441 gtaatgctct gtattcaggt cgcctttgcg ataacgacga ttttaatgct gtggtcggtg
     1501 tcgattaaag ccgcctcgtt gctgggcgat catagtgagc aagggaaaat tatgggctgg
     1561 atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg tgtttaccat gtgggtcttt
     1621 tctcgctttg caccggatga cagcgccagc ctgaaaacag tcattatcat ctacagtgtg
     1681 gtttacatct tgttggggat tctgtgctgg ttttttgtta gcgataacaa caacctgcgc
     1741 agtgccaata acgaagaaaa acagtcattc cagcttagcg acatccttgc cgttttgcgt
     1801 atcagcacca cctggtattg cagcatggtg atttttggtg tcttcacaat ctacgccatt
     1861 ctgagttact ccaccaacta tctgaccgaa atgtacggca tgtcgctggt ggcagcgagc
     1921 tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt gcggcccact tggcggcatt
     1981 atcaccacct acagtaaagt gaaatcccct acccgcgtga tccaaatcct ttccgtactc
     2041 ggcctgctgg cgttaactgc cctgctcgtc acgaactcta acccgcaatc ggtcgcgatg
     2101 gggattggcc tgattttact gctgggattc acctgttacg cctcacgcgg gctgtactgg
     2161 gcctgccctg gcgaagcgag aacaccgtct tacattatgg gcaccacggt aggtatttgt
     2221 tcggtgattg gattcctgcc ggatgtcttc gtttacccga ttatcggcca ctggcaagac
     2281 accctgcccg cagcagaagc ctaccgcaat atgtggctga tgggaatggc ggcgcttgcc
     2341 atggtgattg tctttacctt tttgctgttc caaaaaattc gtactgctga tagcgccccc
     2401 gcaatggcta gcagcaagta agctaggaaa attacaggag aaaaatcatg tcgaaaaaat
     2461 acatcatagg gattgatggc ggaagtcaga gcacaaaagt ggtgatgtac gatctggaag
     2521 gtaacgtggt ttgcgaaggc aaaggcttat tacagccgat gcacacgcca gatgccgata
     2581 ctgcagaaca tcctgacgac gatttatggg catcattatg ttttgccggt cacgatttga
     2641 tgagtcagtt tgctgggaat aaagaagata ttgtcggtat tggtctggga tccatccgtt
     2701 gctgtcgtgc gttattgaaa gccgatggca cgcctgctgc gccgttgatt agctggcagg
     2761 atgcacgcgt tacacgccct tacgaacaca ctaatcctga cgtggcatat gtcacctctt
     2821 tttcgggtta tctgacgcat cgcttaaccg gcgagtttaa agacaatatc gccaactatt
     2881 ttggtcagtg gccggtggat tataagagct ggacatggag cgaagatgct gcggtaatgg
     2941 ataagtttaa tatcccccgt catatgctgt ttgatgtgca aatgcctggc accgtcctcg
     3001 gacatatcac accacaagcc gcactggcga cacatttccc ggcaggactg ccggttgttt
     3061 gtaccaccag tgataaaccg gtagaagctc tgggggccgg attactggat gatgaaactg
     3121 cggtaatttc tcttggcact tacatcgcat tgatgatgaa cggcaaagca ctgccgaaag
     3181 atccggtggc gtactggccg attatgtctt ctattccgca aacattgctg tatgaaggtt
     3241 acggtattcg caaaggtatg tggacggtga gctggctgcg tgacatgtta ggcgagtcgt
     3301 taattcagga tgccagggcg caggatcttt caccggaaga tttactcaac aagaaagcct
     3361 ctagtgtgcc acctggctgt aatgggctga tgacggtgct ggactggctg accaatccgt
     3421 gggaaccgta caaacgcggg attatgatcg gctttgattc cagcatggat tacgcatgga
     3481 tatatcgttc gatattggaa agcgtggcgc taacgctgaa gaacaattac gacaatatgt
     3541 gtaatgaaat gaatcacttt gcgaagcatg tgatcattac tggcggcggt tcgaacagcg
     3601 atctgtttat gcaaattttt gccgacgtgt tcaaccttcc ggcacgccgt aacgccatta
     3661 acggttgtgc aagtctggga gcagcgatta atacagcggt aggtctgggg ctatacccgg
     3721 attacgcaac ggctgttgat aagatggttc gcgtgaaaga tatctttata ccgattgaga
     3781 gcaatgccaa acgctacgac gcgatgaata aaggcatttt caaagaccta accaaacata
     3841 ctgatgtgat cctgaaaaaa tcgtatgaag tgatgcatgg ggaattgggg aatgtggatt
     3901 cgatccagag ctggtcgaat gcgtaagtga ggaaggccgg gcgggaaact gcccggcctg
     3961 aacatacctg aatggttatc cccgctgacg cggggaacat aagttcgaca acttaaaaaa
     4021 tgataacaag tccggtttat ccccgctgat gcagggaaca taataaaaac tttaactagt
     4081 tcggtttatc cctgttttta caaggaatat taactacact cgatgtcttt agaataaaag
     4141 acaatacgaa tcgcacttta tcaattttca cttaaagtat gaactggata tctttattca
     4201 taagaactat tcatcaacag cgtatagagg cggttatgta aaaccactca ttagcctcaa
     4261 aacaaagaca aacttccaga tttattactt aaccaatgaa taacaagctt aaaaaacata
     4321 ctgttattct aaagaa
//
LOCUS       DAZFWP020000265         4287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFWP020000265 DAZFWP020000000
VERSION     DAZFWP020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.267"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            169..1602
                     /locus_tag="ACHK0Z_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     CDS             169..1602
                     /locus_tag="ACHK0Z_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4504741.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            2423..2986
                     /locus_tag="ACHK0Z_RS24440"
                     /old_locus_tag="pgaptmp_004888"
     CDS             2423..2986
                     /locus_tag="ACHK0Z_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4504742.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            3141..>4287
                     /locus_tag="ACHK0Z_RS24445"
                     /old_locus_tag="pgaptmp_004889"
     CDS             3141..>4287
                     /locus_tag="ACHK0Z_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HGU4504743.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQH"
BASE COUNT         1199 a          809 c         1039 g         1240 t
ORIGIN      
        1 tggtgaataa tacagtcttc actgaattaa ccttatcggt ttattaaaca tcagatctac
       61 aaatgctctg tatttctcag agcatcaggt ctgaatagca gctattttcg ctattgtagc
      121 gggatttgct gctattttac taacccatta atgaaaagag aggcttttat gtgcttactt
      181 gcaccagaaa atccttatcc gatatatgca ctaccaccgc tggtgagaaa tgcaataatt
      241 gaaactcaaa aaaatacaca ggcacctttg gctatggtgg caacatccgc attaacagcg
      301 atctcaattg cctgtcagaa tcagattgac gtgtgcagac ctggaaattt acatgggcct
      361 gttaatcttt actctctgat tctggctgat tccggtgaaa ggaaaacgac cgtggataaa
      421 gtgtttatga aagcatttta tctcagggat gaagccctgg cggatgaata cgcgaaactg
      481 gttgagaatt acagtacaga aaaggaaata tgggagcaaa aacaaaaagc gctggaatca
      541 aaatttcata aagagattcg tgccggtaaa gattacaagg caacagaatc agagcttgaa
      601 acgcatctga ataaacctcc tgttccgccg cagatacgtc gaacaatttt taatgagaca
      661 acgatagagg gaatgttaaa atattactcc gatagcaatc gctcttttgc tcttgtatcc
      721 agtgaagggg gggtaatttt tgacagccgg gccatgagta aactgggaat tattaacact
      781 ttgtgggatg gaggttctct tttcatcgac aggaaatcat ctcccggaat taatttgaag
      841 gagccaagac tgacgatatc ggtgatgatt cagcctgatg tttaccacaa aggtttttgt
      901 acgcgaaaaa aagaaattgt gaaaacgtca ggacatcatg caaggttttt gatgtgtcaa
      961 ccaacatcaa cgcaggggac aaggataata accggagata attattcatc acagtatcag
     1021 gatttgtttg aagagaggat caatgagctt attgatgaaa gtctggcgat gagtggtgaa
     1081 cgacgttgcc ttcacttttc tcctcaggct gccagaatct ggacggatta ttacaatgat
     1141 gtggaatcga agctgggggg attggggcct ttaagacatt gccgggaata tgctgctaaa
     1201 aatgcagagt atatggcaag actggcagga cttatttacc attcgagcgg tgaagagggg
     1261 gagatttccc cttacactgc agaaatggcg agagaattag caatatggta cggtaatgag
     1321 tatgtgcggt tgtctaatcc gttaactttt gacaactctg ccctgaccgt acctgtgcga
     1381 ctcattccag aggagcttga acttttcaac tggataaaaa gctattgtat tgagaagggg
     1441 atcctctgta tgaaaaaaaa tgacatttta cagcgtggtc cgaaccgttt ccggaaaaag
     1501 gataaaatca actggttact ggatttactg tatgaacaaa acagagttgt accggttatt
     1561 gagggaaaaa cgttgtgtgt cgcacctaac tttgacctct gaacgactta atcgttgagt
     1621 acatatagtc tcctctgcta cttgcggagg agactaatgt ctttaccatg tcagcgttgt
     1681 attagaaaac tctggtacag gtcagtcagt aaatataact ggtgtaacca ctatttacta
     1741 ttggatagag tgttactcag gatattgtcg gctttagtct gaggtgaggt ggggccttca
     1801 ttgttttcgt gttcaataaa taatttaggt taattagtat tattagtgga agttatgtga
     1861 tctgttatgt tgtcttgtac gcacttaaca ctggttacgt accggaacac tattagagta
     1921 tgttatggct ctgttacagt ttataggtat gaaaaaatat aatctttcct gtatgaaata
     1981 atattcttat ggtgtgtggg gtatggtctg tataccatca tttttatggt gaagttaaat
     2041 atctgtagag gcctcaagct gggtcgctgt ttttacttaa tggtacagaa gtattaatag
     2101 cggtgttgcc tgtggctacc cgatactggc ttttgtgaat gtcagattac ttcctgtaag
     2161 tgattgaatg gtacttcctt aaactcaatt ctatgaggtg tgtttttacc attgtaattc
     2221 gtactggtcc tcagtttaag tgagtcttac tgtactctga aatattacct ctttggagta
     2281 atgaggtaat acgaaacagt tttatcaggt tgtctgtgct gctggttacc tcgtttatta
     2341 atgccgcggt acggcgtttc tgtactctca gtatttatgt aattccacct tcctgtaact
     2401 tttataaagt aaggactata aaatgtataa tgctaatccc aattacgaaa tggacttcat
     2461 gattctgaaa gacgtgaatg aacacatgga aggtctgttt caacgttttt ctaaactgtt
     2521 acctttcaga attgattttg cctacaggaa agacacgccc agttttggtc attcctgtag
     2581 gcacagtatg tgtattgaaa tgcaccgctt acttagtgaa acccaaacga tgctggcggg
     2641 atattactgg gtaatggaat atacgtcaaa caagggactt catattcatt ttatcggcta
     2701 cctggatggg caacgtcaca acaaatctta tcggatatcc aggcaactgg gagatatctg
     2761 gaggcggatc acggaagggg agggttattt tcacctgtgc agggctaaag acaaataccc
     2821 ggtgcgtatc gatcacgtaa ttcactatag tgataaatct gcggtagacg acttacgtta
     2881 cgcccttagc tatctggcca aacaggatca gaaagaacac ggtattattc tgaggcgtag
     2941 caggcttccc gaaaaaagta accggggccg accacgacat aactgaatta cagctgtatt
     3001 aggtattaga attaagtttc tgtaagttgt tgggaggtgc ttgagttaat aggcagaaga
     3061 gggcggggcg ctttgtcatc agtgtagggg acccgaattt ctgccgtggt gcctcagtgg
     3121 atcggataca ggtaacgaat atggttcata aatctgacag tgatgaatta gctgcattga
     3181 gggcagaaaa tgcccgtctg gtctcattac ttgaagctca tgggattgaa tggcgacgta
     3241 aaccgcagac tcctgtgcag cgcgtttctg tattatccac cgacgagaag gttgcattat
     3301 ttcgtcggtt gtttcgtggg cgtgatgatg tatgggcact tagatgggaa agtaaaacca
     3361 gcggcaaatc agggtactct cctgcctgcg ctaacgaatg gcaggcgaga atatgcggta
     3421 aaccccggat aaaatgtggg gactgcgctc accgtcagtt gattcctgta tccgatctcg
     3481 tcatctacca ccatctggcc ggtacccata ctgtcgggat gtatccgttg ctggaagatg
     3541 attcctgtta ttttctggca gttgattttg atgaagctga atggcagaag gatgcatccg
     3601 cattcatgcg atcctgtgat gagctgggtg tacctgctgc gctggaaata tcccgttcac
     3661 gtcagggggc gcatgtctgg atattttttg cctcacgagt ttcggcccgc gaagctcgcc
     3721 gtctggggac tgctattatt agctatacgt gtagtcggac ccgacagctg cgattggggt
     3781 cttatgaccg attatttcct aatcaggata ctatgccaaa agggggattt ggtaatctca
     3841 tagcgttacc tctgcaaaaa agaccgcgtg aattgggggg aagcgttttt gttgatatga
     3901 atctccagcc ttatcctgat cagtgggctt ttcttgtatc ggtgaccccg atgaatgtgc
     3961 aggatattga accgacgata ttacgggcta cagggagtat ccatcctctg gatgtgaatt
     4021 ttatcaacga agaagacctg ggtacgccgt gggaagagaa aaaatcatca ggaaacagac
     4081 tgaatatttc tattgcagaa ccgctgaaaa tcacgctggc aaaccagatc tatttcgaaa
     4141 aagcgcaatt acctcaggtg ctgattaacc gacttattcg gctggcagca tttccgaacc
     4201 ctgagtttta taaggctcag gcaatgcgta tgtcagtctg gaataagccc cgtgttatag
     4261 gttgtgcgga gaattacccg caacaca
//
LOCUS       DAZFWP020000266         4277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFWP020000266 DAZFWP020000000
VERSION     DAZFWP020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.268"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..123)
                     /locus_tag="ACHK0Z_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             complement(<1..123)
                     /locus_tag="ACHK0Z_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HGU4504744.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMY"
     gene            complement(176..1432)
                     /gene="xapB"
                     /locus_tag="ACHK0Z_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             complement(176..1432)
                     /gene="xapB"
                     /locus_tag="ACHK0Z_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HGU4504745.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(1492..2325)
                     /gene="xapA"
                     /locus_tag="ACHK0Z_RS24460"
                     /old_locus_tag="pgaptmp_004892"
     CDS             complement(1492..2325)
                     /gene="xapA"
                     /locus_tag="ACHK0Z_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HGU4504746.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2574..3338
                     /gene="yfeN"
                     /locus_tag="ACHK0Z_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             2574..3338
                     /gene="yfeN"
                     /locus_tag="ACHK0Z_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HGU4504747.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3377..>4277)
                     /gene="yfeR"
                     /locus_tag="ACHK0Z_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             complement(3377..>4277)
                     /gene="yfeR"
                     /locus_tag="ACHK0Z_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4504748.1"
                     /translation="VFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENHTGVRLLDRT
                     TREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQTISAHLIPQC
                     IAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCEAILSEPFFL
                     LCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQANIVQEIGHPA
                     TLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEAL
                     WDVVRDQGNALMAGREGDPLYQI"
BASE COUNT         1195 a         1128 c          956 g          998 t
ORIGIN      
        1 atacataaac acaatggata atatacctgg taatacattt ggtacaggaa taggcaagcg
       61 cacgattgta aacaaagcac caatgccaaa accaacgccg gaagaaaata aaatatcttt
      121 cactaattaa cctttatcat aaaagcagct ctgaagagca gagctgcgaa tccttttaat
      181 gagccaccgc tcgatgcttt atcttttcag ggtcatgatt atatttaaac ccaaagaaaa
      241 atatcactgc gagaaaaaga gcatatcctg caaacaccag ccagatagtt tgccagtctt
      301 ttacgccatc caccgaaaag taatctactg ccatgccact cagaatcgag ccaacccatg
      361 cgccgacacc atttaccatg gtcataaaga gcccctgcgc gctggcacga atgctggaat
      421 caacttcctg ttcgacaaat accgaaccag aaatattgaa gaaatcgaat gcacagccat
      481 aaacaatcat cgacagcagc agcaaaataa atccggttgt tgacggatcg ccataggcga
      541 agaagccaaa gcgcagcgtc caggccacca tactcatcag catgacggtt ttaatgccaa
      601 atcgctttaa aaagaatggg atagtcagta taaagcccac ttctgccatc tgtgaaactg
      661 acagtaaaat ggagggatat ttcaccacaa aactgtcagc aaactccggg ttacgggcga
      721 aatcatgtag gaacggatta ccaaaaacgt tggtaatttg cagtaccgca cccagcatca
      781 tggcaaagag gaaaaagatg gccatgcgtg gatttttaaa cagcacgaag gcatccagac
      841 ccagcttgct ggcaagcgat gtggtcgctt ttttctccgc aaccggaatc ttcggcaaag
      901 tcagcgcata agccgacagc agcaatgacg caccggacgc gatatacagc tgcagactac
      961 tcaattccag atgcagcagg cttactgccc acatcgcgac aatgaacccc accgtaccaa
     1021 aaacgcgaat gggcgggaaa gcggtcaccg ggtcaagccc tgcctgggca agacaggaat
     1081 aagagacgct gttcgataac gcaatagtcg gcataaacgc catcgcattg actaacatca
     1141 cccaaaacat catatccgga tcagttacgg atgccgcata aaaaagtacg cccgcacaca
     1201 ccaggtgaca cagcatgtat gcacgttctg cgcgcagcca tttgtctgcg atgatcccca
     1261 ttataccagg cataataatc gcggcgatcc ctttggaact gtaaaccatg ccaacattag
     1321 cgccggtgaa atgaagggta ttaatcatgt aagagccgag ggtaaccagc cagctccccc
     1381 agataaaata ttgcaaaaag gacattacct ttaagcgcat cgcgatgctc ataatcgttt
     1441 ccttgtcgca tcatttggcc tcacccgcga ggccgtttct tattgttttt ctcaggcaat
     1501 tttgcgcaga aagccacaaa taaggttgat gaagttctgc ttagagagct ccgcagccgc
     1561 cagcgtttgc gcatgtgaca atttcacatc gcttaaacct tccgccatat tggtaatcgc
     1621 agagaccgca acgactttaa ggtcgcaatg gcgagctgaa ataacctcag gcaccacaga
     1681 cataccaaca acatccccac caataatttg catcatgcga atttccgccg cagtctcgaa
     1741 attcggcccc ggatacgaga cgaacacgcc ctccgtcaga gggaaaccct cttctttcgc
     1801 cactttttgt aacagtgcgc ggtattccgc atcgtaggca tttgccagtg agaagaagcg
     1861 ctctccaaaa cgatcatcgt taagacccac catcggcgta ccgggcatgg tgttgatatg
     1921 atctttcaat gcgaccaggc tgcctgcccc cacttccgga cgcagcgagc ctgccgcatt
     1981 ggtgcagaac agtagctcgc agcccagcag cttaaaggta cgaattgcgt cagtcataat
     2041 ggtcattcca cgaccttcgt agaaatgtcc gcgacctttc atacatacca ccggtacgcc
     2101 ctggagatgg cccagcacta actcccccgc atgaccatgt accgtactta ccgggaagcc
     2161 cggcagcttt tcgtaggaaa ttgcgacagc gttctcaatc tgatcggcca gcgcgccgag
     2221 cccggaacct aaaataaagg ccactcgtgg cgtgaaatca ggtttataag tcttgataat
     2281 atctatgcaa aacagcgggt tatgagaaaa ttgaacctga gacatacata tcctttttct
     2341 gtagggtgga atctaacgcc tttttatagc gaggttgtcc cggctcttac caatacactt
     2401 ttctcacgca ttcaatagcg attcgatatc gatggattta tatcttttct ctattaatcc
     2461 acggtaaaaa ctgtattggc gatattaatt cctttgcatt aacaatatgt cgtttttagc
     2521 aaaccacttt attaagcaat ctactaataa cgatcggcat agggaattat gctatgaaaa
     2581 aacatctttt aactctgaca ctttcctcta tattagcgat acctgttgta tctcatgcag
     2641 aatttaaagg cggttttgca gatatcggcg tgcattatct ggactggacc agccggacta
     2701 ccgaaaaatc gtcgaccaaa tcacacaaag atgattttgg ttatcttgaa tttgaaggtg
     2761 gcgctaactt tagttgggga gaaatgtacg ggttcttcga ctgggaaaac ttctataacg
     2821 gtcgccataa taaaccaggt agcgagcagc gttatacctt taaaaatacc aaccgtattt
     2881 acctgggtga caccggattt aatctctact tgcatgcgta tggcacctac ggttctgcga
     2941 atcgcgtgaa tttccacgac gatatgttcc tgtacggtat cggctacaat ttcaccggca
     3001 gcggttggtg gttcaaaccg ttctttgcta agcgttatac agatcaaacc tattacactg
     3061 gcgacaacgg ctatgtcgcc ggctgggttg caggttacaa ctttatgctg ggcagcgaga
     3121 aattcactct taccaactgg aacgagtacg agtttgaccg tgacgctacc tatgcagcgg
     3181 gtaatggcgg taaagaaggg ctgaatggcg cagttgcact gtggtggaat gcaacatcac
     3241 acattactac ggggattcag tatcgatatg cggatgacaa actgggcgaa gatttttacc
     3301 aggatgcgat aatctactcc atcaaattta acttctgatt gattactgcc ggggctcccg
     3361 gcagtatgaa ttcactctat atctgataca acggatcccc ttcccgacca gccattagcg
     3421 cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa agtgaacggt
     3481 tcttcctgcg caccagcatc aactggcgtt caacaaccgg cgtaatgcgc tttaccacca
     3541 gtgggctacc ttcaggcaat ggtagcgcca gtgcgggaag aatactgatg ccgatgcctg
     3601 cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa
     3661 taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca
     3721 acaccaattt cgctccctgt aatgcctgcc agggtacata atcttcgacg gccaaagcgc
     3781 tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat tgcagatcgc
     3841 ccactgggcc tggatcgatg acgatgccaa agtcgacatc tccctggcga atactttcca
     3901 tcacccactg ctgcggacga tcgtgcagga caaactgaat atctggatag cggcgatggc
     3961 tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa
     4021 cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttcagttcat
     4081 ccagcagtcg ctcaagacgc aaagccagct gctgccctgc atctgtaagc accacttcac
     4141 gcgtggttct gtccagcagg cgaacaccag tatgattttc cagttccttc acactgtgac
     4201 tcactgccga ctggctcagg ccgatacgct ctcctgcacg actaaaactt ttctcctgcg
     4261 ctactgtgac gaaaacc
//
LOCUS       DAZFWP020000267         4191 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFWP020000267 DAZFWP020000000
VERSION     DAZFWP020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.269"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..385
                     /locus_tag="ACHK0Z_RS24475"
                     /old_locus_tag="pgaptmp_004895"
     CDS             <1..385
                     /locus_tag="ACHK0Z_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HGU4504749.1"
                     /translation="DLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPN
                     FAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNK
                     QQAVVTASKPKLDLTNTDWIYGVDL"
     gene            382..1083
                     /locus_tag="ACHK0Z_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             382..1083
                     /locus_tag="ACHK0Z_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4504750.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            1080..1370
                     /locus_tag="ACHK0Z_RS24485"
                     /old_locus_tag="pgaptmp_004897"
     CDS             1080..1370
                     /locus_tag="ACHK0Z_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU4504751.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            1444..1884
                     /locus_tag="ACHK0Z_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     CDS             1444..1884
                     /locus_tag="ACHK0Z_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4504752.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQPTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            1881..2408
                     /locus_tag="ACHK0Z_RS24495"
                     /old_locus_tag="pgaptmp_004899"
     CDS             1881..2408
                     /locus_tag="ACHK0Z_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4504753.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTIVS
                     KAALMAIGQGVRRAT"
     gene            2405..2587
                     /locus_tag="ACHK0Z_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     CDS             2405..2587
                     /locus_tag="ACHK0Z_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4504754.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            2584..2754
                     /locus_tag="ACHK0Z_RS24505"
                     /old_locus_tag="pgaptmp_004901"
     CDS             2584..2754
                     /locus_tag="ACHK0Z_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU4504755.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            2747..3358
                     /locus_tag="ACHK0Z_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             2747..3358
                     /locus_tag="ACHK0Z_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4504756.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            3355..4020
                     /locus_tag="ACHK0Z_RS24515"
                     /old_locus_tag="pgaptmp_004903"
     CDS             3355..4020
                     /locus_tag="ACHK0Z_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HGU4504757.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            4017..4088
                     /locus_tag="ACHK0Z_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /pseudo
     CDS             4017..4088
                     /locus_tag="ACHK0Z_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT         1217 a          961 c         1127 g          886 t
ORIGIN      
        1 cgacctttct gtggtgaaac ctgatgctgc aattcagagc ggcagcaaat gggggacagc
       61 agaagacctg accgccgcag agtggatgtt tgacatggtg aagactatcg cgccatcagc
      121 cagaaaaccg aattttgcag ggtgggctaa cgatatccgc ctgatgcgtg aacgtgacgg
      181 acgtaaccac cgcgatatgt gtgtgctgtt ccgctgggcc tgccaggaca acttctggtc
      241 cggtaatgtg ctgagcccgg ccaaactccg cgacaagtgg acccagctcg aaatcaaccg
      301 taacaagcaa caggcagtcg tgacagccag taaaccaaaa ctcgacctga caaacacaga
      361 ctggatttac ggggtggatt tatgaaaaac atcgccgcac agatggttaa ctttgaccgt
      421 gagcagatgc gtcggatcgc caacaacatg ccggaacagt acgacgaaaa gcctcaggta
      481 cagcaggtag cgcagatcat caacggtgtg ttcagccagt tactggcaac tttcccggcg
      541 agcctggcta accgtgacca gaacgaactg aacgaaatcc gccgccagtg ggttctggct
      601 ttccgggaaa acgggatcac cacaatggaa caggttaacg caggaatgcg cgtagcccgt
      661 cggcagaatc gaccatttct gccatcaccc gggcagtttg ttgcatggtg ccgggaagaa
      721 gcatccgtta tcgccggact gccaaacgtc agcgagctgg ttgatatggt ttacgagtat
      781 tgccggaagc gaggcctgta tccggatgcg gagtcttatc cgtggaaatc aaacgcgcac
      841 tactggctgg ttaccaacct gtatcagaac atgcgggcca atgcgcttac tgatgcggaa
      901 ttacggcgca aggctgccga tgaactgtcc tgtatgaccg cacgaattaa ccgtggtgag
      961 gctatacctg aaccagtaaa acaacttcct gtcatgggcg gtagacctct aaatcgtgca
     1021 caggctctgg cgaagatcgc agaaatcaaa gctaagtttg ggctgaaagg agcaagtgta
     1081 tgacgggcaa agaggcaatt attcattacc tggggacgca taatagcttc tgtgcgccgg
     1141 acgttgccgc gttaacaggc gcaacagtaa ccagcataaa tcaggccgcg gctaaaatgg
     1201 cacgggcagg tcttctggtt atcgaaggta aggtctggcg aacggtgtat taccggttcg
     1261 ctactaggga agaacgggaa ggaaagatga gcacgaacct gatttttaag gagtgtcgcc
     1321 agagtgccgc gatgaaacgg gtattggcgg tatatggagt taaaagatga ccatctacat
     1381 cactgagcta ataacaggcc tgctggtaat cgcaggcctt tttatttggg ggagagggaa
     1441 gtcatgaaaa aactaacctt tgaaattcga tctccagcac atcagcaaaa cgctattcac
     1501 gcagtacagc aaatccttcc agacccaacc aaaccaatcg tagtaaccat tcaggaacgc
     1561 aaccgcagct tagaccaaaa caggaagcta tgggcctgct taggtgacgt ctctcgtcag
     1621 gttgaatggc atggtcgctg gctggatgca gaaagctgga agtgtgtgtt taccgcagca
     1681 ttaaagcagc aggatgttgt tcctaacctt gccgggaatg gctttgtggt aataggccag
     1741 ccaaccagca ggatgcgtgt aagcgaattt gcggagctat tagagcttat acaggcattc
     1801 ggtacagagc gtggcgttaa gtggtcagac gaagcgcgac tggctctcga atggaaagcg
     1861 cgatggggag atcgggctgc atgactatca aatcaaatac gccagcacac gacaaggact
     1921 gctggcaaac gccgctttgg ctttttgatg cactggatat tgagtttgga ttctggctgg
     1981 attcggcagc gagcgacaaa aacgctctgt gcgctcactg gctaactgag gctgacgacg
     2041 cgctaaattc tgagtggata agccacggtg caatctggaa taacccaccg tacagcaata
     2101 tcaggccgtg ggtggaaaaa gccgctgagc agtgcataca acagcgacag acggtagtga
     2161 tgcttgtgcc agaggatatg tctgtcggat ggttcagcaa ggctctggag agtgttgacg
     2221 aagttcgtat tatcactgat ggacggatta attttatcga accatcgaca gggctggaga
     2281 agaagggaaa cagcaaaggc tccatgctgc tgatttggcg accgttcatt agtcctcgac
     2341 ggatgtttac tatcgtatcc aaagcggcat tgatggcgat cgggcagggc gtcaggaggg
     2401 cgacatgagg cgacaacgac gaagtttcac cgacatcatc tgcgaaaact gcaaatacct
     2461 tccaacgaaa cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac
     2521 cttcaactac acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaatac
     2581 aaggtgattg acccaaatcg aagttacgaa caagaaagcg tcgagcgggc tttaacgtgc
     2641 gctaattgcg gtcagaagct gcatgtgctg gaagttcacg tgtgtgagca ctgctgtgca
     2701 gagctgatga gcgattcgaa tagctcgatg cacgaggaag aagatgatgg ctaaaccagc
     2761 gcgaagacga tgtaaaaacg atgaatgtcg ggaatggttt caccctgcat tcgctaatca
     2821 gtggtggtgc tctccagagt gtggaaccaa gatagcactc gaacgacgaa gtaaagaacg
     2881 cgaaaaagcg gaaaaagcag cagagaagaa acgacgacga gaggagcaga aacagaaaga
     2941 taaacttaag attcgaaaac tcgccttaaa gccccgcagt tactggatta aacaagccca
     3001 acaagccgta aacgccttca tcagagaaag agaccgcgac ttaccatgta tctcgtgcgg
     3061 aacgctcacg tctgctcagt gggatgccgg acattaccgg acaactgctg cggcacctca
     3121 actccgattt gatgaacgca atattcacaa gcaatgcgtg gtgtgcaacc agcacaaaag
     3181 cggaaatctc gttccgtatc gcgtcgaact gattagccgc atcgggcagg aagcagtaga
     3241 cgaaatcgaa tcaaaccata accgccatcg ctggactgtc gaagagtgca aggcgatcaa
     3301 ggcagagtac caacagaaac tcaaagacct gcgaaacagc agaagtgagg ctgcatgaat
     3361 atctacgaaa gaattgatgg cagcaaatac cgaaatattt gggtagctgg cgacctgcac
     3421 ggatgctaca cgaacctgat gaacaaactg gatacgattg gattcgacac caaaaaagac
     3481 ctgctcatct cggttggcga tttggttgat cgcggtacag agaacgtcga atgtctggaa
     3541 ttaatcacat tcccctggtt cagagctgtg cgtggaaacc atgagcaaat gatgattgat
     3601 ggcttatcag agcgtggaaa cgttaatcac tggctgctta atggcggtgg ctggttcttt
     3661 aatctcgatt acgacaaaga gactctggct aaagttcttg cccataaagc agaagaactt
     3721 ccgttaatca tcgaactggt gagcaaagat aaaaaatatg tcatttgcca cgccgattat
     3781 ccttgtgata aatacgagtt tggaaagcca gttgatcatc agcaggtaat ctggaaccgc
     3841 gaacgaatca gcaactcaca agatgggatc gtgaaagaaa tcaaaggcgc ggacacgttc
     3901 atctttggtc atacgccagc agtgaaacca ctcaaatttg ccaaccagat gtatatcgat
     3961 actggcgcag tgttctgcgg aaacctcaca ttgattcagg tacagggaga aggcgcatga
     4021 gactcgaaag cgtagctaaa tttcattcgc caaaaagccc gatgatgagc gactcactac
     4081 tggccacagt ttattggttt tcgtaactga gtcattttat tattttattg caacttttaa
     4141 tcttttatag tgcgaaataa atggagctgg cattcatttc gcactttatg t
//
LOCUS       DAZFWP020000268         4126 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFWP020000268 DAZFWP020000000
VERSION     DAZFWP020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.270"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..412
                     /gene="grlA"
                     /locus_tag="ACHK0Z_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             <1..412
                     /gene="grlA"
                     /locus_tag="ACHK0Z_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HGU4504758.1"
                     /translation="ESKNKNGDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDIA
                     EAFGINLRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESP
                     VSTGPKRKNYRVGNGIVGQSNIWNEMIMRRKKEN"
     gene            complement(798..1253)
                     /gene="cesD"
                     /locus_tag="ACHK0Z_RS24530"
                     /old_locus_tag="pgaptmp_004906"
     CDS             complement(798..1253)
                     /gene="cesD"
                     /locus_tag="ACHK0Z_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGU4504759.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(1267..2805)
                     /gene="escC"
                     /locus_tag="ACHK0Z_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             complement(1267..2805)
                     /gene="escC"
                     /locus_tag="ACHK0Z_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU4504760.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISNSVNDDFSGEIKNEKPMKVLEKLSRLYHRTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            complement(2805..3260)
                     /gene="sepD"
                     /locus_tag="ACHK0Z_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             complement(2805..3260)
                     /gene="sepD"
                     /locus_tag="ACHK0Z_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HGU4504761.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPTHLFSDIGRIV"
     gene            complement(3266..3838)
                     /gene="escJ"
                     /locus_tag="ACHK0Z_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             complement(3266..3838)
                     /gene="escJ"
                     /locus_tag="ACHK0Z_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HGU4504762.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(3840..>4126)
                     /gene="escI"
                     /locus_tag="ACHK0Z_RS24550"
                     /old_locus_tag="pgaptmp_004910"
     CDS             complement(3840..>4126)
                     /gene="escI"
                     /locus_tag="ACHK0Z_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner rod protein
                     EscI"
                     /protein_id="HGU4504763.1"
                     /translation="TPSAAGAAQFLDQLLPKTAGVSSPEQVLIEEIKKRHLATMNTDL
                     SFDALSAGGLSPEDVLTLQKNVLNANVNVDVVSKLASLLSTSVTKLVSMQ"
BASE COUNT         1208 a          750 c          788 g         1380 t
ORIGIN      
        1 ggaatctaaa aataaaaatg gcgactatgt aattcctgac tcagtaaaga attacgatgg
       61 tgagcctctg tatatcttgg tttctctttg gtgtaaattg caggagaaat ggatttctcg
      121 caatgatatt gccgaagcat tcggtataaa cctgaggaga gcatcattta ttataactta
      181 tatatcgaga agaaaagaaa aaatttcatt tcgtgtcaga tatgttagtt atggtaactt
      241 gcattataag cgccttgaga ttttcattta tgatgttaac ctcgaggctg ttccgataga
      301 aagtccggta tcaaccggac caaaaagaaa aaactaccga gtcggtaatg gtattgtggg
      361 gcagtctaat atctggaacg aaatgatcat gaggcggaaa aaggagaatt aaacgtattt
      421 ttttgagtat attttataga agaataaaaa taaagctcta ttcaagggct ttgttttcca
      481 ggataagcct ttttctctgc caaagtttga aagtcatttc ctgacaaata cactctgggc
      541 tatagatgat tgaaacagtc agatttctga tgctaagtta agatatatac tgcttattct
      601 ataagcggaa atgacattga gtacagcaga agatggttca ggcgcataaa atcctggtta
      661 atggagtaac ccactcctgt agataaatct gtcagcggtc ggtgctcaac gttgggcgca
      721 cttcaccaaa gcagctttat tatttccgat agaaaatccc cagacagcct ggggattggt
      781 atggcagatg gtagttttta atctgtatta cctaacaatt ttttaatatt ttctttatat
      841 gtagcgtatt tttcttgttc attacaccac attaaacaag ccttaaaggc ttttttggcc
      901 attttccgat ttccgaccaa taagtagcta atcccagaat aataagatgc cctgggatct
      961 tgcataacta aagttgcaca gcgagcgaaa cacagttgtg catcttcatg atttgataaa
     1021 cgctgatgac acaaccccag agatagggtg tagtcataat tccagtgctc cagatatgtt
     1081 agcagatgaa atagatttct tgcggttata acatcaccag attgatatgc tgtataggca
     1141 taagaatgaa gatcattgag atcttgttgc gtcaaatttg tcagtgatgc taacgtacca
     1201 ccatcctggt agaaatcata gatatcctgt agagagctaa atttcctgct cataaagcct
     1261 catttattat tcgctagatg ctgattttat cggggtcgct ttaatcaaaa agagtcgaac
     1321 aacgctgtgt ttttgttttg ttgtactgga gaataagtta ccaataacag gaatatcact
     1381 taacaacggt ataccatctt tacttgaact ctctttatcc tggataaatc cgccaagcag
     1441 cagactttct ccagcgctta atgtcgcttc tgttgtcatt tctgaatttt gtacttcagg
     1501 taagatgctg ctggcatcct gagcattact ttggttagtc gattgattgc catcctgaat
     1561 atcaagtaac aaacgaactc gttcccgacg ttttccggta agactattcg agttgtcatc
     1621 aagtatacgt ggtgtcactc tcagtaatgt tccgctggta atactttcca gagatgccac
     1681 cttttctccg gaaacttttg tataaaacgt tacgttttta tcaagaatcg cctgcatatt
     1741 atttaaggtt ataattgatg gctgagagag tatttttgct ttcgagttct gttgtaacgc
     1801 attcactctg atcatgaaat tattggcgtt gcttatcaca gaagaactga gattagcttg
     1861 cgccgttgag ctgttaaagg caatagaacc ttgccctgca tttaatgtac cggaccagtt
     1921 tactcccagt tgctgaagat catttgcatc aacatcaata attgaaacag aaatctcaat
     1981 ttgcctttgc tcaatatcaa gctcagaaat taattgccga tatatgtcca tagttatttc
     2041 acgatctttt acaacaactg cattaagacg tggatctgcg gaaatcttta cactgttatc
     2101 aaacaggttg ccgtttcttt ctacagcacc tttgcctgtg ctgggaagtg agccattact
     2161 tgccatcgtc ttcaggatgg agacgacgcc aggcacgaca acattttggt cgcggtattt
     2221 atacgttata tctgtcgctg atgcatagtt taatttaaat actctaacga catctttatt
     2281 ttttgcttta gactgtgctt ctttatcaag actttcagat aaagatgtaa tgtattttat
     2341 gcattcagga acgccacgaa cttcaatcga gttaaatgtt gtgatttttc tcacgttaca
     2401 ggagttttta tttactgaga ttgtatcacg cagatatttt aacagtgagt caatatccag
     2461 atatgttggc gtaattatgc ttcttgatat ttcatttgtt ttataaatat acaaaatgtt
     2521 ctcatcataa taccatgtgc gatgatagag cctggacagt ttttccagta ctttcattgg
     2581 tttttcattt tttatttcac cagagaaatc atcattcaca gaattactga tgaaaacagg
     2641 gatgctataa ttagcggcaa aatcattgag gattgcacgt accggagagc tttttgtaat
     2701 aataaaatac tcgttcttgc ctaatctttt ttccagagag gacggggcag cctgagcact
     2761 gcagcaaaat agtgctgtaa aaataaaaaa acttattttt ttcattacac aattcgtcct
     2821 atatcagaaa acaaatgtgt tggaggcatc gttgtatctg atgtagatgc ttgctctaac
     2881 ttatcaaatt gcttgtcaag caaagaggcg agtgtgtgtt gcattattgc ttttgacatg
     2941 tttgcgcgat gattattatc tttattaaac agttggcaaa gataccattt tttattagat
     3001 tgaaaaatgc ccatttgttt atattcttta ctaacggcca gaatattaca gagtctgatg
     3061 aaggtatctg ttggaacctg gcgaaaatcg ccattaagct cgatatacag aacccagtaa
     3121 cttccaaagt cgtgtaagaa ggcatttttt tgatagatat gaaaagtaag atgctttggt
     3181 gaacctttga catcttttac aaaatcaaga gccgttagcc agtcactatc attcttaaca
     3241 atgccattat tattgttcat acatattacc cgtcctgtcc tgaggatgac ttgataacga
     3301 cactaatatt ttctagtttg agatcgtcta cgctattctt aactaagttt tttatctgaa
     3361 tgactgatgg tgctaagtta acctctggcg agctaatgac taaaacagcc gctgaggatg
     3421 gttggctctc gttattgtta acatttaatg aaacgctgca atcgataaca ccggggattt
     3481 tacttagtaa cctttctata tcttgttctt tgagatagtt gatttttgca ttttcttgtg
     3541 atggcgatgc cactagctga ctgggcggaa aaataacttc aatatctgca aatttctttt
     3601 tcgggaagcc attgttattt aaaatggtta tcgcacggac aaaatcctct ttttccacac
     3661 ttattgttac atttccactt ttgtccattt ctttagatac attgacatca ttggataaca
     3721 acaatgcttg catctgattg gcttcttttt ccgttaatcc tgtatacaat tgctctttac
     3781 atgcaactgt caaacaaata aaaacaaata aaaaggtgtt tttaatgtat tttctcattt
     3841 tattgcattg aaaccagttt tgtaaccgat gttgaaagaa gacttgctaa cttggatacg
     3901 acatcaacat tgacgtttgc gttgagcaca ttcttttgta atgtgagcac atcttctggc
     3961 gagagtccac ctgcagatag ggcatcgaaa ctgagatcgg tgttcattgt tgcaagatgt
     4021 ctctttttaa tttcttcaat taaaacttgc tctggagaag atacaccagc tgttttagga
     4081 agtaattgat caagaaactg agccgctcca gctgcagagg gcgttg
//
LOCUS       DAZFWP020000269         4121 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFWP020000269 DAZFWP020000000
VERSION     DAZFWP020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.271"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2805
                     /locus_tag="ACHK0Z_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             <1..2805
                     /locus_tag="ACHK0Z_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4504764.1"
                     /translation="LASENPDEAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDS
                     QPGTLNDFLGAMTEDDARPEALRRFEQMVEEVARNASVVAQNTAAAKKSASDAGTSAR
                     EAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEAEKSAAAAESSKSAA
                     ATSAAAAKTSETNAAASQQSAATSASTATTKASEAATSARDASASKEAAKSSETNASS
                     SASSAASSATAAANSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAASTSA
                     GQASASATAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETS
                     AESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAA
                     QSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESLAATPK
                     AVKAAYELANGKYTAQDATTAQKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYT
                     AQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSARKVNGKALSADI
                     TLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGI
                     SELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWD
                     YTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYAL
                     MQGQTFDKSAYPKLATAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASA
                     SSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGS
                     VHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNA
                     AGNAENTVKNIAFNYIVRLA"
     gene            2805..3380
                     /locus_tag="ACHK0Z_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             2805..3380
                     /locus_tag="ACHK0Z_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4504765.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSID
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(3478..4068)
                     /locus_tag="ACHK0Z_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             complement(3478..4068)
                     /locus_tag="ACHK0Z_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4504766.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         1094 a         1036 c         1098 g          893 t
ORIGIN      
        1 ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga gtacgggcag
       61 tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac catcaccgtg
      121 tatgaagatt ctcaacccgg tacgctgaat gattttctcg gtgccatgac ggaggatgat
      181 gcccgtccgg aggcactgcg ccgttttgaa cagatggtgg aagaggtggc gcgtaacgcg
      241 tccgtggtgg cacagaacac ggcagccgcg aagaagtcag ccagtgatgc cggcacatct
      301 gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc agccagcacg
      361 tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg aacggcatca
      421 acaaaggcca ctgaagcgga aaaaagtgct gccgctgcag agtcctcaaa aagcgcggca
      481 gctaccagtg ccgctgccgc gaaaacgtca gaaacgaatg ctgcagcgtc acaacaatca
      541 gccgccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac ctcagcccgg
      601 gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc ctcgagcgcc
      661 agtagcgcag cttcctcggc aacggcggca gcaaattccg cgaaggcggc aaaaacgtcc
      721 gagacgaacg ccaggtcttc tgagacggca gcgggacaga gcgcctcagc tgcggcaggc
      781 tcaaaaacag cggctgcgtc gtctgccagt gccgcgtcaa caagtgccgg gcaggcctca
      841 gccagtgcca ccgctgccgg aaaatcggca gaaagcgccg catcgtccgc ttcaacagcc
      901 acaacgaagg ctggcgaagc cactgaacag gccagcgcag cagcgaggtc tgcttccgca
      961 gcgaagacat ccgagacgaa cgcgaaagcg tcggaaacca gcgcagaatc ctcaaaaacg
     1021 gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catctgcgtc tgcttcaaaa
     1081 gatgaggcga ccagacaggc gtcagcagca aagggcagcg ccacgacggc atccacgaag
     1141 gcgacagagg cagctggcag tgcgacggcg gcagctcaga gcaaaagtac ggcggaatcc
     1201 gcggcaacgc gcgccgagac agcggcaaaa cgggcagagg atattgcatc cgccgtggcg
     1261 cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagcagtgc gactaacagc
     1321 acttccgagt cactggcggc aacgccaaaa gccgttaagg ccgcgtatga gctggctaac
     1381 gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggatagttca gctcagcagc
     1441 gcgaccaaca gcacatctga aatgctggcg gcaacgccaa agtcggtaaa ggcagcctat
     1501 gaccttgcta acgggaaata tactgctcag gacgctacga cagcacaaaa aggaattgtc
     1561 cagctcagta gtgcaaccaa cagcgcatct gaaacgcttg ccgcgacacc gaaagcagtg
     1621 aaagcagcta atgataatgc gaatggtcgg gtaccttctg cccgtaaggt gaatggtaag
     1681 gcgctttcag cggatataac actgacgccg aaagatattg gtacgcttaa ctcaacaaca
     1741 atgtcattca gcggtggtgc tggttggttc aaattagcaa cggtaaccat gccacaggcg
     1801 agttctgttg tttcaattac gttgattggt ggcgcgggat ttaacgtggg gtcacctcaa
     1861 caggcaggta tatctgaact tgttttgcgt gcaggtaatg gtaatccgaa ggggattact
     1921 ggtgctttat ggcagcgcac atcgacaggg tttacaaatt ttgcctgggt caatacatct
     1981 ggtgatactt acgatattta cgttgcaatc ggaaattatg cgactggtgt aaatattcaa
     2041 tgggattata ccagtaatgc cagcgtgact attcatacgt caccagcata ttctgctaat
     2101 aagccggaag ggttaacgga cggtacagtt tattcactct atacaccatc agagcagttt
     2161 tatccgcctg gcgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg
     2221 atgcaggggc agacttttga caaatccgct tacccgaaac ttgcaaccgc ttatccgtca
     2281 ggcgtgatcc ctgatatgcg tggctggacg attaagggca aacctgccag tggtcgggcc
     2341 gtattgtctc aggaacagga cggcattaaa tcgcacaccc acagcgccag tgcatccagt
     2401 acggatttgg ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac
     2461 accggggcgc atacgcacag tgtgagcggt acagccgcaa gtgccggaaa ccatactcat
     2521 agtgtcacag gcgcatcagc agtcagccag tggtcacaaa atgggtcagt acataaggta
     2581 gtgtctgcgg ccagtgtgaa tacaagtgct gcaggagcgc acactcatag tgtcagcggc
     2641 acagctgcat ctgcaggtgc tcacgcacat actgtcggta ttggtgctca tacgcactct
     2701 gttgcgattg gctcacatgg acacaccatc accgttaacg ctgctggtaa cgcggaaaac
     2761 actgtcaaaa acatcgcatt taactacatt gtgaggcttg cataatggca ttcagaatga
     2821 gtgaacaacc acggaccata aaaatttata atctgctggc aggaactaat gaatttattg
     2881 gtgaaggtga cgcatatatt ccgcctcata caggtctgcc agcaaacagt atcgatattg
     2941 caccgccaga tattccggca ggcttcgtgg ctgttttcaa cagtgatgag gcatcgtggc
     3001 atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat
     3061 ttatttctga actcggtccg ttaccggaaa atgttacctg gttatcgccg ggaggggaat
     3121 atcagaagtg gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgga
     3181 tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catattgcgc
     3241 cgcttcagga tgctgtggat ctggagatcg caacggagga agaaaccttg ttgctggaag
     3301 cttggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca ccggatattg
     3361 aatggccagc cctaccgtag ggtaagatat ataaattcta taattagaag tatctttcca
     3421 tttaaggcta ggaagggggg cttggaaaac gtaaggaatc tcacaccgag attattttta
     3481 tatatcaggt gtctgatttt ttgctttagc tcttaaaatg gtttgccgcg aggttttgaa
     3541 ttcttgggca atgtcactta tatttatacc tgacttaatt tgctctagta ccctctgtat
     3601 ttgttcatca tttaacacag gtgggcgacc aaatcgcttt cctgcgcccc gtgctcttaa
     3661 tatccctgaa tgagtacgct caagtaaaag gtctcgctca aattcagcga ctgctgaaat
     3721 tacttgcatc atcatttttc caggtggact ggtcaggtca acgcccccca atgctaagca
     3781 atgcactttg atacctgctt tggtcagttg ttccaccgtt ttactgatat ccattggatt
     3841 acaaccgagg cgatccagtt ttgtgacaat caatgcatca cctcttttca gtcgatcaag
     3901 caactggtta aaaccaggac gctcactggt tgctgctgag ccgctaattt gttcttcaat
     3961 tatttgctga gatttgatgt taaaacctgc actttcgatt tcccgacgtt gatttttggt
     4021 tgtctgttcc agagttgata cccgacagta agcataaatt cgagacatag tgagatcttc
     4081 tatacgaaat tggtgtacat atcataatgc atctcagaaa a
//
LOCUS       DAZFWP020000270         4089 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFWP020000270 DAZFWP020000000
VERSION     DAZFWP020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.272"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..333
                     /gene="cesT"
                     /locus_tag="ACHK0Z_RS24570"
                     /old_locus_tag="pgaptmp_004914"
     CDS             <1..333
                     /gene="cesT"
                     /locus_tag="ACHK0Z_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HGU4504767.1"
                     /translation="EYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCYESGA
                     QSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSD
                     NKHYYAGR"
     gene            394..3240
                     /gene="eae"
                     /locus_tag="ACHK0Z_RS24575"
                     /old_locus_tag="pgaptmp_004915"
     CDS             394..3240
                     /gene="eae"
                     /locus_tag="ACHK0Z_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type epsilon"
                     /protein_id="HGU4504768.1"
                     /translation="MITHGFYTRTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADNVDTITYTATVKKNGVAQANAPVTFSIVSGTATLGANSAKTDGNGKATVTL
                     KSGTPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKANGSDAITYIV
                     KVMKNNQPEANHSVTFSTNFGNLGGNSNTQIVKTDKDGRATVKLTSGVAGNAVVSAKV
                     SEVNTEVKAPEVKFFSVLSIDSNVSIIGTSANGALPNIWLRYGQFKLTAKGGDGKYQW
                     RSQDPSVASVDALTGRVTLLKKGTTTIEVVSGDNQTAMYTINTPTKFISVETQNKVVY
                     SDAEATCRMNNARLPSSTSELKDVYNKWGAANSYEGYKGKKTITAWTQQTEDDKQKGW
                     TSTFDIVTKNEIPSNGSNSKVHVNKANAFAVCVR"
     gene            complement(3514..>4089)
                     /gene="escD"
                     /locus_tag="ACHK0Z_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             complement(3514..>4089)
                     /gene="escD"
                     /locus_tag="ACHK0Z_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU4504769.1"
                     /translation="AINDILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLL
                     LNTPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKI
                     LAIINAMNKNSNVKILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSI
                     LKGGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT         1227 a          770 c          894 g         1198 t
ORIGIN      
        1 gaatatatga tgatctatgg cgtctgtggg aaattcccga cagataatcc taacttcgct
       61 cttgagattt tgaatgcaaa cttatggttt gcagagaatg gtggaccata cctgtgttat
      121 gaatcaggag cacaatcgct gttgttagcg ttacgttttc cgctcgatga tgctacacct
      181 gaaaaacttg agaatgaaat agaagtcgtc gttaagtcaa tggaaaacct gtatttggta
      241 ttacataatc agggaataac attagaaaac gaacatatga aaatagagga aattagttca
      301 agcgataata aacattatta cgccggaaga taaaagatta tttattaata taatttacta
      361 ttcattctaa ctcattgtgg tggagcccat aacatgatta ctcatggttt ttatacccgg
      421 acccggcaca agcataagct aaaaaaaaca tttattatgc ttagtgctgg tttaggattg
      481 tttttttatg ttaaccagaa ttcatttgca aatggtgaaa attattttaa attgagttca
      541 gattcaaaac tgttaactca aaatgccgct caggatcgcc ttttttatac gttaaaaaca
      601 ggtgaaactg ttgccaatat ttctaaatca caggggatca gtttatcggt aatttggtca
      661 ctgaataaac atttatacag ctccgaaagc gaaatgatga aggctgggcc tggtcagcag
      721 atcattttgc cactcaaaaa actgtctgtt gaatatagtg ccttacctgt cttaggttcg
      781 gcacctgttg ttgctgcagg tggtgtcgct ggtcatacga ataaaatgac taaaatgtcc
      841 ccggacgcga ctaaaagcaa cacgaccgat gacaaggctc taaattatgc ggcacaacag
      901 gccgcgagcc ttggtagcca gctccagtcg cgctcactga acggcgatta cgcgaaagat
      961 accgctcttg gtatggccag cagccaggct tcgtcacagt tgcaggcctg gttacaacat
     1021 tatggaacgg cagaggttaa tctgcagagt ggtaataact ttgacggtag ttcactggac
     1081 ttcttattac cgttctatga ttccgaaaac atgctggcat ttggtcaggt cggggcgcgt
     1141 tacattgact cccgctttac ggcaaattta ggtgctggcc agcgtttttt ccttcctgaa
     1201 aatatgttgg gctataacgt cttcattgat caggattttt ctggtgataa tacccgttta
     1261 ggtattggtg gcgaatactg gcgagactat ttcaaaagta gcgttaacgg ctatttccgc
     1321 atgagcggct ggcatgagtc atacaataag aaagactatg atgagcgccc ggcaaatggt
     1381 tttgatatcc gctttaatgg ctatttacca tcatatccgg cattaggcgc caaactgatg
     1441 tacgaacagt attatggtga taatgttgct ttgtttaatt ccgataagtt gcagtcgaat
     1501 cctggcgcgg cgaccgttgg tgtaaactac actccgattc ctctggtgac gatggggatc
     1561 gattaccgtc atggtacggg taatgaaaat gatctccttt actcaatgca gttccgttat
     1621 cagtttgata aaccgtggtc tcagcaaatc gagccacagt atgttaacga gttaagaaca
     1681 ttatcgggca gccgttacga tctggttcag cgtaataaca atattattct ggagtacaaa
     1741 aagcaggata ttctttctct gaatattccg catgatatta atggtactga acacagtacg
     1801 cagaagattc aattgatcgt taagagcaaa tatggtctgg atcgtatcgt ctgggatgat
     1861 agtgcattac gcagtcaggg cggtcagatt cagcatagcg gaagccaaag cgcacaagac
     1921 taccaggcta ttttgcctgc ttatgtgcaa ggtggcagca atatttataa agtgaccgct
     1981 cgcgcctatg accgaaatgg taatagttct aataatgtac agctcactat taccgtttta
     2041 ccgaatgggc aggttgtgga ccaggttggg gtaacggact ttacggctga taagacatcg
     2101 gctaaagcgg ataacgttga taccattact tataccgcga cggttaaaaa gaatggtgta
     2161 gctcaggcta atgcccctgt aacatttagt attgtatccg ggactgcaac tctcggggca
     2221 aatagtgcca aaacggatgg taacggtaag gcaaccgtaa cgttgaagtc gggtacgcca
     2281 gggcaggtcg tcgtgtctgc taaaaccgcg gagatgactt cgccacttaa tgccagtgcg
     2341 gttatatttg ttgatcaaac caaggccagc attactgaga ttaaggctga taaaacaaca
     2401 gcgaaggcaa atggttctga tgcgattacc tatattgtta aagtaatgaa gaataaccaa
     2461 ccagaagcaa accattctgt tacattctca acgaactttg gtaatctggg gggaaattct
     2521 aatacccaaa ttgtgaaaac ggataaagat ggtagggcta cggtaaaact gacatctggc
     2581 gttgcaggta atgctgttgt tagtgcaaaa gtcagcgaag ttaatacaga ggttaaggct
     2641 cctgaggtaa aattcttctc agttctgagc attgatagta atgtgagtat tattggaacc
     2701 tccgctaatg gcgctttacc taatatttgg ttgcgatatg gtcagtttaa gctgacagcc
     2761 aaaggtggcg atgggaaata tcaatggcgc tctcaagatc caagtgttgc atcagttgat
     2821 gctttaactg gtcgagttac tttgctgaag aaaggaacaa caacaattga agttgtatcg
     2881 ggtgataacc aaacagcaat gtatacaatt aatacaccta caaaatttat atctgtggag
     2941 acacaaaata aagtagtcta tagtgatgct gaggcaacat gtagaatgaa taatgcacgc
     3001 ttgccgtcat ctacgagtga gctaaaggat gtgtataata aatggggcgc cgccaatagt
     3061 tatgaaggct ataaaggtaa aaaaacaata acagcatgga cacagcaaac tgaggatgat
     3121 aaacaaaaag gttggactag tacatttgac atagttacaa aaaatgaaat ccctagtaat
     3181 ggcagtaata gtaaagtcca cgtgaataaa gctaacgctt ttgccgtctg tgtaagatga
     3241 tcttataatc attaattaat gcccaactaa gatatttatc ttggttgggc ttaatagata
     3301 tttattcaac cttaatagtt gtaccttatt taggtaaatt tctttacggc gaggccatta
     3361 ttgaattgcc caactgcgct tgtttaatat tctgtataaa tgcttctctc agaactgacc
     3421 atatttttcc accgctccct attagaaaat ttgctattac ttcaccctac aataaataaa
     3481 aaaccctccg aagaggggga agacgataat aaattaatac gatagcggaa tatgtatata
     3541 ttcgtctggg cgggagatgt tgataccatc tttaggatca atcgcgtcta tgacatagcc
     3601 tcctttcaga atgctaccaa ttcccaggct tgtaccgtta tcaagagcaa ttgttggatt
     3661 cttcatcgtg ccgctgattc tgagtatttt tccggggaaa atatctgctg aaatgtacgg
     3721 ctgaatgttc tgaaatagta tcttgacgtt actatttttg ttcatcgcat ttataatggc
     3781 aaggatctta ctctcgttct gttgagaaac ctcccccgca acaatgatga catcattctt
     3841 tttgaaaata ttgacatagt ttattaattt attctttttg aactctgatg ccagttcatt
     3901 gattttattg ttactgttgt tatgtatttc ccatccagca acccctggcg tattcagtaa
     3961 cagattttca acttcgctcc atttgggtga agggggaatg tatcccgata acaggaaaaa
     4021 tccaggctta ttaccttttg taatgatgat atccttataa ccgtattccg ttaaaatatc
     4081 attaatcgc
//
LOCUS       DAZFWP020000271         4083 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFWP020000271 DAZFWP020000000
VERSION     DAZFWP020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.273"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..386)
                     /locus_tag="ACHK0Z_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     CDS             complement(<1..386)
                     /locus_tag="ACHK0Z_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HGU4504770.1"
                     /translation="MATLMEKDALLNGASQCITFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELA"
     gene            complement(531..1880)
                     /gene="lysC"
                     /locus_tag="ACHK0Z_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     CDS             complement(531..1880)
                     /gene="lysC"
                     /locus_tag="ACHK0Z_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU4504771.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(1972..2168)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2405..4054
                     /gene="pgi"
                     /locus_tag="ACHK0Z_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     CDS             2405..4054
                     /gene="pgi"
                     /locus_tag="ACHK0Z_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU4504772.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1047 a         1045 c         1026 g          965 t
ORIGIN      
        1 ttggctaatt caatcagaga tgatttacct gctccgggtt gcccacctaa aacaaaacca
       61 atgggatgct gttgcggtgt gacttcatga tttgtcagac gctgccaaat agaatcaaaa
      121 gatgctttat tttctgaatg cgtagtattg ttgaattcat gctgatattg agttcttacc
      181 tgctgaagga gaatcttacc ttgtgtaaaa tcaacaagtt cagcgggggt agaataaaga
      241 taatcgcgat agctgactaa tcgcttgaga gcatcgcctg aatcctgatg cgacgaaact
      301 gagcaattat caatgatatt tgataaaaac gtgatgcact gactggctcc gtttaaaagt
      361 gcatcttttt ccattaaagt agccattaac actcctgtta aatgatattt ttcggtgatt
      421 ataatactgt tctacaggag tgattttgct tttcagatat tatttaatct atgttttcag
      481 tgaggatggc gtataacaat cggtgagcgt tatacgccag tagggaaata ttactcaaac
      541 aaattaaaat gcagcttctg caccacctgc tcggcatctt cgccgggcac caggaagcac
      601 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
      661 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
      721 gccagacctt cttctacttc cacccggcac agtgcggaaa gctccatcag cagagattgc
      781 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
      841 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
      901 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
      961 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
     1021 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ctgggatatc gctgcgtact
     1081 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
     1141 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
     1201 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
     1261 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
     1321 ctaccgataa atccctgggt gatcactaag ccgtcattga ggcgtgggag cagctgcagc
     1381 gcggccagtt ccgccagcgc ggctacatct ggctctgcac gaccaaatcg gtcgttggta
     1441 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatttcg
     1501 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
     1561 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
     1621 tcttcacgga taacgttcgg gtaacgcaaa cgttccagaa tggcaaactg gatgttgcga
     1681 atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc cttccgctaa agcgaccagc
     1741 agattagtga taccagcaga agccgagagg actactaaac gcacgttggc atcagaaagc
     1801 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
     1861 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
     1921 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
     1981 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
     2041 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
     2101 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
     2161 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
     2221 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
     2281 gcttcactgc gctaagggtt tacactcaac attacgctaa cggcactaaa accatcacat
     2341 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
     2401 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
     2461 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
     2521 taagttctcc gcaaccttcg gcgatcagat gctggtggat tactccaaaa accgcatcac
     2581 tgaagagacg ctggcgaaat tacaagatct ggcgaaagag tgcgatctgg cgggcgcgat
     2641 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgctg tgctgcacgt
     2701 agcgctgcgt aaccgtagca ataccccgat tttggttgat ggcaaagacg taatgccgga
     2761 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
     2821 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
     2881 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaatcacc tgaacatgca
     2941 ctttgtttct aacgtcgatg ggactcacat cgcggaagtg ctgaaaaaag taaacccgga
     3001 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
     3061 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
     3121 ctttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
     3181 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
     3241 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
     3301 gatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtac tgctggcgct
     3361 gattggcatc tggtacaaca atttctttgg tgcggaaact gaggcgattc tgccgtatga
     3421 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
     3481 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
     3541 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
     3601 accgtgcgat ttcatcgctc cggctatcac ccataacccg ctctctgatc atcaccagaa
     3661 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
     3721 ggttgagcag gaatatcgtg atcagggtaa agatccggca acgcttgact acgtggtgcc
     3781 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgtg aaatcactcc
     3841 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
     3901 cctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacaac tggcaaaccg
     3961 tattctgcca gagctgaaag atgataaaga aatcagcagc cacgatagct cgaccaatgg
     4021 tctgattaac cgctataaag cgtggcgcgg ttaatttaaa aaccttaata acaatgccga
     4081 cgt
//
LOCUS       DAZFWP020000272         3925 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFWP020000272 DAZFWP020000000
VERSION     DAZFWP020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.274"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            70..1728
                     /gene="dpiB"
                     /locus_tag="ACHK0Z_RS24600"
                     /old_locus_tag="pgaptmp_004920"
     CDS             70..1728
                     /gene="dpiB"
                     /locus_tag="ACHK0Z_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU4504773.1"
                     /translation="MLQLHENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1697..2377
                     /gene="dpiA"
                     /locus_tag="ACHK0Z_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /gene_synonym="citB"
     CDS             1697..2377
                     /gene="dpiA"
                     /locus_tag="ACHK0Z_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU4504774.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2418..3803)
                     /gene="dcuC"
                     /locus_tag="ACHK0Z_RS24610"
                     /old_locus_tag="pgaptmp_004922"
     CDS             complement(2418..3803)
                     /gene="dcuC"
                     /locus_tag="ACHK0Z_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4504775.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1069 a          938 c         1015 g          903 t
ORIGIN      
        1 ctatgtattt tgattcaaca ttttaattac atccgtcaaa gaggctcggg acaacccgca
       61 aggaaaacaa tgttgcagct tcacgagaat aaacagtttg catttttcca aagactggca
      121 tttccgctgc gtatcttttt gctgattctg gtgttctcaa tatttgtcat tgcagccctg
      181 gcgcaatatt ttacggccag ttttgaggac tatttaacgc ttcatgtacg cgacatggca
      241 atgaatcagg cgaaaattat tgcctccaat gacagtgtca tctctgcggt gaaaacgcgt
      301 gactacaaac ggctggcgac catcgctaac aaattacaaa gagataccga ttttgattat
      361 gtggtgattg gggaccggca ctcgatccgc ctttaccatc ctaatccgga gaaaattggt
      421 tatcctatgc agttcaccaa acagggcgcg ctggagaaag gggagagcta cttcattacc
      481 gggaaagggt caatggggat ggcgatgcgc gccaaaacgc caatctttga tgacgatgga
      541 aaagtcatcg gcgtggtgtc gattggctac ctggtgagta aaatcgatag ctggcgggct
      601 gagtttttat taccgatggc aggcgtgttt gtcgtgctgt tagggattct gatgttgctg
      661 tcgtggttcc tggcagcgca tatccgtcgg caaatgatgg gcatggagcc aaagcaaatc
      721 gcacgcgtgg tccgtcagca agaggcgctg tttagttcgg tttatgaagg gctgattgcg
      781 gtggatccgc atggttacat taccgccatc aatcgtaacg caagaaagat gctggggctg
      841 agctctcccg gacggcaatg gttgggtaaa cccattgctg aagtggtcag gcccgccgat
      901 ttctttaccg aacagattga tgaaaaacgt caggatgtgg tggctaactt taacggtctg
      961 agcgttattg ccaatcggga agctattcgt tcaggtgatg atttgctggg ggccattatc
     1021 agctttcgta gtaaagacga aatttccacc ctcaatgcgc aactgacgca aataaaacaa
     1081 tacgtcgaga gccttcgtac attgcgacac gagcatctca attggatgtc gacgctcaat
     1141 ggtctgttgc agatgaaaga gtatgatcgc gtgctggcga tggtgcaggg ggagtctcag
     1201 gcccagcaac agcttattga cagcctgcgt gaggcgtttg ccgatcgcca ggtggcgggg
     1261 ctgctttttg gtaaagtgca gcgcgcccgg gaactggggc taaaaatgat cattgtcccc
     1321 ggtagccagc tttcgcaact gccgccagga ctggatagca ccgagtttgc agccattgtg
     1381 ggcaatttac ttgataacgc cttcgaagcc agcctgcgta gcgatgaagg aaacaagatc
     1441 gttgaattat tcctcagcga tgaaggcgat gatgtggtga ttgaagtcgc cgatcagggc
     1501 tgcggcgttc cagagtctct acgagacaaa atatttgagc agggggtcag tacgcgtgct
     1561 gacgagcccg gtgaacatgg cattgggttg tacttgattg ccagctacgt aacgcgctgc
     1621 ggtggtgtta tcactctcga agataatgat ccctgcggta ccttgttttc aatctatatt
     1681 ccgaaagtga aacctaatga cagctccatt aaccctattg atcgttgagg acgaaacgcc
     1741 gctggcagag atgcatgcgg aatatattcg tcacattccc ggattcagtc agatattact
     1801 ggcgggaaac ctggcgcagg cccgaatgat gatcgagcgt tttaagccgg ggctaatctt
     1861 gctcgataac tatcttcctg acggcagagg gattaattta ttgcatgaac tggtgcaggc
     1921 gcattatccc ggcgacgtgg tgtttaccac tgcagccagc gatatggaaa cggtgtctga
     1981 agccgtacgt tgtggtgtat ttgattatct cattaagccc attgcctatg aacggctggg
     2041 gcaaacgcta acccgtttcc gccagcgtaa acatatgctg gaaagtattg atagcgccag
     2101 ccagaagcaa attgatgaga tgtttaatgc ctatgctcgc ggtgaaccta aagacgaact
     2161 gccgaccggc attgatccct tgacgctaaa cgcggtgcga aaactgttta aagagcctgg
     2221 tgtgcaacat acggcagaaa cggtggcgca ggcactgacc atcagtcgca ccactgccag
     2281 gcgttatctt gaatattgcg ccagccgcca tctgattatt gctgaaattg ttcacggcaa
     2341 agttggcaga ccgcaacgca tataccacag tgggtgacat gataaagccg gaggaaactt
     2401 gcctccggca ttactattta cttgcctgtg accgctgctg ccgttcctgg caccatcagc
     2461 tctgtagcaa cgataacaat caccaaacca acaagcaccg gtaccgaggt gcgttttacg
     2521 acttcaaacg gcgagatctt cgccatcccg gcaaccgcaa cgactacgcc agaaacgggc
     2581 gaaagggtac ggcccaggtt tgacgcctgc agcatcggga tagtcaaata cgccgggtta
     2641 atgccggaag agtgcgccag tttcgggatc atctcaacaa acgcataaaa cggcgcattg
     2701 cctgaaccgg tcgtgactgc cgccagcatg gtcagaatca ccaataccag catcaggatg
     2761 atactcgccg aaccaaacga ggtagcgata gaaatcagac tttgaataaa gccgatggtg
     2821 ctaagcccct gagcgaatac cccagcggca accagcagca tcaccacgtt agcaaacgca
     2881 tctgccattc cgcgataagc cacttccaga ccagagaaaa ctttctgggt attaaagctg
     2941 cggaggaact ccagaatgga ggcaatcagc atacaaatca ccagaatagt gatgatgtgt
     3001 aattgcggac cccatttacc gtcaaaaatc agtacaccga tgatcggcgt gaacggcaaa
     3061 atggcataaa acgcaggagc agtggtggtg atttcactga catctaacat ttcatgagag
     3121 atgtgctctt ttttatccag ataacgttgc cagaagaagt gggcgatcgc catgccgata
     3181 attgcagcaa ttgagatagg cagcgtcgtt ttgaaggcga agtcaatcag cgacatttcg
     3241 gaagcttgcg ccgccagcac cacatcccct gaagtcggtg cgagaataat cgccgccggg
     3301 gaggcacaaa tggcagcagc tgcgccacga ctgataccaa cgtttaccat caccggaaat
     3361 agggttgcca tcagcaaaac acccagaccg gttgcggaag agacggccag agacatcaga
     3421 caggcgacaa aataggcggc aatcatcagc aggtaagggg agttaatata ctgcaatggt
     3481 tttgacgcca gcttgaccac catatcattc gcgccgatat gggtcatgta agcggcaaat
     3541 ccacacagca tcataatcat catgccgagg tcgccgccgc ggctcattag taatatttta
     3601 acgtattcaa cgatatccgt ggcgctgtag cctgttgaag cctggctgga cggtaacact
     3661 ttgtgcccca taatggcact gataatcagc aataacaggc caccgacaaa taacacacca
     3721 gtggcggaat accctttaat gatgtagcga gctacaccca caataaccac aaccccaata
     3781 aggagttcaa tgaatgtcag cataattttt cctgtctcca ggccccaaag taaataataa
     3841 aaaattccta aagcttaagg aaaaaatatg cccaataaat tggcgatgaa tgctgattaa
     3901 aatcaagaaa aactgccatt aagac
//
LOCUS       DAZFWP020000273         3897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFWP020000273 DAZFWP020000000
VERSION     DAZFWP020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.275"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(64..3798)
                     /gene="yfaL"
                     /locus_tag="ACHK0Z_RS24615"
                     /old_locus_tag="pgaptmp_004923"
     CDS             complement(64..3798)
                     /gene="yfaL"
                     /locus_tag="ACHK0Z_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU4504776.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGDGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLKNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT          922 a         1119 c          877 g          979 t
ORIGIN      
        1 gctccccgta ggccggataa ggcgtttacg ccgcatccgg caaccgtgcc gactagacag
       61 tgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat catcactccc
      121 tttctgccac gccacgctgc cgcgcaggga cactcgctga ctgatattgc ccgtgactcc
      181 cacttttatt tcaccccgtc gcttcaccgc atcgtcactg atagtgctgc catcttcttc
      241 aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg tgacaccaac
      301 cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc cctgcgattg
      361 tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct gataaatcac
      421 ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc cggcttccag
      481 cgaggcgata atccccgacg agtggtaatg atctgtgcca tcgtcttgtt cggaaacatc
      541 attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct tctgattacc
      601 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
      661 agttccggtc atattcgagc ggctctcgcc ctgattatca ctgtagccac caaccgcccc
      721 aagcatccac tcgccatcat cgccccagcg cccgctaaac aggtcaccgc taagctgcac
      781 cgtagaagtg tcttcatgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
      841 gataacacgt aaattcagcg tctgaccatt gccacctgca tgatcgcgtc gctccatcat
      901 aaacgcctga tttgccgccc gcagattatt gagataaccg ccaactttgg cattcagcac
      961 cggctggtaa gcggggattg gatcgggtgt tgggtcagga tcgggtgttg ggtccgggtc
     1021 tggatcaggt ggcgatggcg gcgttacttc ttgcgatcgc agataccagt cgttgttatc
     1081 ttccactagc gtgtagtcat acgctcccat gttgacgtag ccactgccag ccagactgaa
     1141 ctgtgcattg ttttgaaact gcgtgggatc agctgcgaaa tcaaccactt taatgcctgt
     1201 cgatgtcggc tcaccaatcc ctgtaatgga gttaaccacc acagttgtgt tgccagcagt
     1261 attaccgttc atcaccaatt gatcgcttac cgagtcatcg ccgtttaatt cgctgtcgaa
     1321 cagtagcgta ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc cagcgacgcc
     1381 atttttcagg ctaaccgcac cggacgtatt ggtcagatca ccattaaccg tcacgtcggc
     1441 aagataggtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca acgtcacgcc
     1501 atcggtagca ttcaccagcg tcccatcgcc gttaaacaat gaggcattaa gggcgacgga
     1561 agtatcctgc ccggtcagac gcaaaaccgt accatcgctg atatcgatag tgccgctgga
     1621 agtagcatcg atagactgaa tatcctgatc cgcgccagta acgaacgtcg ccccatcacc
     1681 gacccacagt gacgaagcat aaggaaagat atccgcaaca tcgcctttca gggtgccttc
     1741 ttcgacacgt actgccgact gcgttgtacc gctggcggtc agctccagcg tacccgcccc
     1801 cgttttagta aatgtgctac cctcatcctg atgctgcccg ctgctgtcag ccatcagtgc
     1861 gccccactgc gtgtctaccc ccgcatcaac tgccacttca ccgtcggcgc gcatttcaat
     1921 atcacgccca tgctcagtcg tcgtagagcg agtatcgaca ttggcagtaa tctccatcac
     1981 gctttcttgt tgcttatcgg taaagataac ctggcggtta tagtgggtat ctccaagctg
     2041 cgagttgtcg ctcaccatca gcgtaccgga ggcgatttgt gttgtgccca aataactatt
     2101 attgtttgcc agacttacct gcccgtcgct cgcatcgacc accacgtaga cgccatcttt
     2161 cccgtcgccg ccgatatgca aattgtcgcc acctgcaaga tctaccacat cctgactgcc
     2221 gataaccgtt ccgctgctgc cactgacttc aaggccatca ttgtatgatg tgccgctctg
     2281 ccaggtggag aaatcagaga gatcgagcac accgccgttt aacacgatcg actgcggatc
     2341 gtcctggaga gcggcgagat ctgccgcgtc gccttccagc gaaagcaccg caccatcatc
     2401 aacgactata tcgccggtta gcgccatcga ctgcgccccc gacagcacgt agctgccgtt
     2461 ttgcgcaatg gtgagctgac cagcaccttc gatggtgcca gcaaaactgc cttgattaac
     2521 agtaacattg ccaccagcat cgatatttaa agtgccattc tgaaagcccg tcaatgagtg
     2581 cacaaaggtt tgttgggtgg agccaacgtt tagctctgcc tgattctggt acttatcaat
     2641 actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg tatcgccgac
     2701 attcatcagg gagttgctgc ggcccagggt aacttcaccg ttttcaatct gcatctcacc
     2761 agtaaagtca ttgttatctg cattaagtac cagatcgccg gaacctgttt tggtgatcaa
     2821 cccggtacca gcaatagagt caacagctcc gtcattctct gtattgccaa taaccagcgt
     2881 ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa agccacccgc
     2941 cgcagtggaa ggaccatctc catagcctgc tgcgctgttg ttctcatcca ctaacacgcc
     3001 gccgttctgg ctgtagctgt catcaacaga aatatcaata agatagggag ccgtcgcgct
     3061 attggtatat atcgccccgc cataaccttc ggcagtgtta tttgtaaagg cagtgttatt
     3121 tattatcgta taacctgaag gatgcttgct gtcgctatta ttatcggtaa catcgattgc
     3181 cccgccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat caaaaataac
     3241 atcacttaaa tagacgtcat tattaatggt ataaattgcg ccacctttgc catcattagc
     3301 gatattattg cgaaacatgg cgttagtgac acgtaaatcg acggcaccag tatcattagt
     3361 accagaagaa tagattgcgc caccatcgcc gcctgcgacg ttaccagaaa aaataacatc
     3421 agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat tatattctcc
     3481 tgtaacggtg ttattggcaa acagagtcat tccagtttca ttttctggta atagtgaaaa
     3541 ttccgctcct tgttgcaaaa atacggctcc tccgctggcg ttattggtca tatccgaaaa
     3601 aaccagccat tgcccatcgg cgacactcca gtcctgcgta atgcctgaaa ggctttgcct
     3661 gctggcgtga caactcgctt tgatatcata tccctggcat aaatcaatgg ccgccgcgaa
     3721 accgttagca gagaaaagag atgcaatcat tgacgggagt agagataaat actccttgcg
     3781 tagaaagata atccgcatat tcatctaacc atcattttat ataagacggc atatttaaac
     3841 gcattataca atatgggtta tctttctttt gtaaattgtc caacccgatg caatggc
//
LOCUS       DAZFWP020000274         3845 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFWP020000274 DAZFWP020000000
VERSION     DAZFWP020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.276"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..236)
                     /gene="ariR"
                     /locus_tag="ACHK0Z_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /gene_synonym="ymgB"
     CDS             complement(<1..236)
                     /gene="ariR"
                     /locus_tag="ACHK0Z_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HGU4504777.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLE"
     gene            complement(265..537)
                     /locus_tag="ACHK0Z_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             complement(265..537)
                     /locus_tag="ACHK0Z_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HGU4504778.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(580..816)
                     /gene="ycgZ"
                     /locus_tag="ACHK0Z_RS24630"
                     /old_locus_tag="pgaptmp_004926"
     CDS             complement(580..816)
                     /gene="ycgZ"
                     /locus_tag="ACHK0Z_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HGU4504779.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1130..2341
                     /gene="bluF"
                     /locus_tag="ACHK0Z_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             1130..2341
                     /gene="bluF"
                     /locus_tag="ACHK0Z_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HGU4504780.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            2546..3277
                     /gene="bluR"
                     /locus_tag="ACHK0Z_RS24640"
                     /old_locus_tag="pgaptmp_004928"
     CDS             2546..3277
                     /gene="bluR"
                     /locus_tag="ACHK0Z_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HGU4504781.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3498..>3845
                     /locus_tag="ACHK0Z_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             3498..>3845
                     /locus_tag="ACHK0Z_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HGU4504782.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
BASE COUNT         1118 a          812 c          779 g         1136 t
ORIGIN      
        1 atttccagtg tttttctaat cacatcagct ttgagaatat cacttgttgt ttccagggag
       61 tgtatcagac ttaagataat atttttatta ttaacattat cgcctgaaag cattaaattg
      121 gtgacagcct gccctaacac tgctgactct tcttctaaca aattacccga actgcgaaag
      181 taacttgcta acgctttatc agttattgca ttatgaattg tagtatcttc aagcatggta
      241 acctctcatc ttacttatga aattttaatg tattctgttt attttcttac cattgaacat
      301 acttttattg tcagctgtcg ctgttcttcg gtttggtcct cctgaatcac tattgctaaa
      361 atcacgaatt atgctgtcaa ttaaatcgcg tcggaaagca tcttcctcag ccgatttcat
      421 tacgagtaat tgcttaataa gagtctgcaa tgaaaaattt atcttcatgc gcagtatctg
      481 gatcaccgcc tggccagtaa tttcatattt tatacgttca ttatcagatg tcttcataac
      541 gacttcatag ctaacctgtt aaataatcag gctgttgcat tattcaaaaa gcaacccaat
      601 tagtgcatta taatgtttct gttcctcttc cccagtcgcg tgttccagtc ggcaaagaag
      661 tttggcacaa agcgactttc gactcagatt acgtccatct ttcaaaatct cagtcacaat
      721 ttccccgaga gtttcctgct gagtaggcaa gttagctttt gcaaagtaac gagtgattgc
      781 tcccgcagaa tctaaagtca ctgaattttg atgcatgcta cgcctctgtt aaaaatgtta
      841 atcaatgtta agtgaggtaa caatagcaaa cttgtacgaa atatgtgtac agaatgataa
      901 attaattttt tgcaattagt gctaatgcat ataaaacaat gtgttatgat aaaacatatt
      961 gcatcattta cctgacaatc tatacaagat ttttcgccaa ctagtgttca actttttcca
     1021 ttttttatca atgtaaagaa acggttatgt tttggtatta aaagaaagac acaagaagat
     1081 atgtctgtta ccgtcttact ctcgcctcac ccattaccct ggattgttaa tgcttaccac
     1141 ccttatttat cgtagccata tacgtgacga cgaacctgtc aaaaaaatcg aagaaatggt
     1201 ttcgatagca aatcgcagga acatgcagtc tgacgtaaca gggatcttac tgtttaatgg
     1261 ttctcatttt ttccagcttc tggaaggtcc ggaagaacag gttaaaatga tatatcgggc
     1321 tatatgccag gatccacggc actataatat tgttgagctg ctgtgcgatt acgcgcctgc
     1381 tcgccgtttt ggcaaagcgg gaatggaatt atttgatttg cgcctgcacg agcgagatga
     1441 cgttttacag gccgtattcg acaaaggcac atcaaaattt cagctaactt atgatgacag
     1501 agcgctacaa ttttttcgta cttttgtcct tgcaaccgaa caatcaacct atttcgagat
     1561 ccctgccgaa gactcctggc tttttatcgc tgacggatct gataaagaac ttgattcctg
     1621 tgccctttca ccaactataa acgaccactt tgcctttcat cctattgtcg atcccttatc
     1681 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagatagcc catcagccat
     1741 agcggttggg cagcgtaaag acggggaaat ctacacagcg gatctcaaaa gtaaggcgct
     1801 tgcattcacg atggcacacg cacttgagct cggcgataaa atgatttcaa tcaatctatt
     1861 acctatgacc ctggttaacg aacctgacgc agtctctttt ttacttaatg aaataaaggc
     1921 caatgctctg gtgcctgaac aaatcatcgt tgaatttact gaaagtgaag tcatatctcg
     1981 gtttgatgag tttgccgaag cgattaaatc gctaaaggct gctggtatca gtgtagcaat
     2041 tgatcatttt ggcgcaggtt ttgctggttt gttactcctg tcacgcttcc agcctgacag
     2101 aattaaaatc agtcaggaat tgattaccaa tgttcataaa agtgggccac ggcaggcaat
     2161 tattcaggcg atcataaaat gctgtacatc acttgaaatt caagtcagtg ctatgggcgt
     2221 ggcaacacca gaagagtgga tgtggcttga atccgcagga attgagatgt ttcagggaga
     2281 tctgtttgct aaagctaaat tgaatggtat cccttccata gcgtggccag agaaaaaata
     2341 attttcagca cattctttca catgatttca gtaaatcatc gtgaaaatga atacgttacg
     2401 tttaaattta atccacatta aaaataatgt ttcatccaat ttagctgaaa tcttatttat
     2461 actttgtaca attttagcgt atattgtaca aagtattatt gggtcgtgta caggcgacgg
     2521 agatttgtga ccgcaaggag gaattgtggc ttattacagc attggtgatg ttgctgaacg
     2581 ttgcgggatt aatcctgtca ctctccgggc ctggcaacgc cgctacggtt tgttaaaacc
     2641 acagcgcagt gaaggcggac accgactctt tgatgaagaa gacatacaac gcatcgaaga
     2701 gatcaagcgt tggataagta atggcgtccc tgtaggcaaa gttaaagcat tactggaaac
     2761 caccagccag gatacggaag atgactggag ccgcctgcaa gaagagatga tgtcaattct
     2821 tcgcatggct aatcctgcca aactacgcgc gagaattatt tcactgggtc gagagtaccc
     2881 agtagatcaa ttgattaatc atgtttacct tcctgttcgc cagcgtctcg tgcttgatca
     2941 caacacctcc cgcattatga gcagtatgtt tgacggcgca ttaattgaat acgcagcaac
     3001 ctcgcttttc gaaatgcgcc gtaagcccgg taaagaagcc attctgatgg cgtggaatgt
     3061 tgaagagaga gcacgattgt ggctggaagc atggcgttta tcattgtcag gatggcacat
     3121 ttctgttctt gctgatccca ttgaatcgcc gcgcccggaa ctgttcccga cgcaaacatt
     3181 gattgtctgg acaggcatgg caccaacgag aaggcaaaac gaacttttac aacactgggg
     3241 tgagcaaggt tataaagtca tctttcatgc cccctaattt cacctttatc ccgcccctta
     3301 gacagaatgt tataatctga acccacaaca gcgtttgtac cggagctggt ccgttgcaaa
     3361 atgctcaccg ggagaattca gatattccct ctatcacact ctgtacatat cacagccaaa
     3421 ggacagacca gattgaagtg gtaaaaccga tcgtgcacac tacgcagtct cacaactaac
     3481 aagaggttat tatcctgatg gatcaggtcg ttgtttttca aaagatgttt gagcaagtga
     3541 gaaaagagca aaacttctcc tggttttatt cagaattaaa acatcaccgt attgcacatt
     3601 acatttatta tctggctacg gataacatca gaattattac tcacgatgac acggttttgt
     3661 tattaagagg aaccaggaac ctgttaaaag ttagtacgac caagaaccct gctaaaataa
     3721 aagaggccgc attgcttcat atttgcggaa aatctacatt tcgggaatac tgttcaacac
     3781 tagcaggcgc gggcgttttc cggtgggtta ctgatgttaa tcataacaaa cgcagttact
     3841 acgcc
//
LOCUS       DAZFWP020000275         3827 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFWP020000275 DAZFWP020000000
VERSION     DAZFWP020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.277"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            35..568
                     /locus_tag="ACHK0Z_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             35..568
                     /locus_tag="ACHK0Z_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4504783.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            842..1537
                     /locus_tag="ACHK0Z_RS24655"
                     /old_locus_tag="pgaptmp_004931"
     CDS             842..1537
                     /locus_tag="ACHK0Z_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HGU4504784.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1632..1763
                     /locus_tag="ACHK0Z_RS24660"
                     /old_locus_tag="pgaptmp_004932"
     CDS             1632..1763
                     /locus_tag="ACHK0Z_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504785.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            1986..2171
                     /locus_tag="ACHK0Z_RS24665"
                     /old_locus_tag="pgaptmp_004933"
     CDS             1986..2171
                     /locus_tag="ACHK0Z_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4504786.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPA
                     PDWQTPLNGIISPSESG"
     gene            2410..2961
                     /locus_tag="ACHK0Z_RS24670"
                     /old_locus_tag="pgaptmp_004934"
     CDS             2410..2961
                     /locus_tag="ACHK0Z_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4504787.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            3307..3633
                     /locus_tag="ACHK0Z_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             3307..3633
                     /locus_tag="ACHK0Z_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HGU4504788.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
BASE COUNT          985 a          822 c         1058 g          962 t
ORIGIN      
        1 gacaggcgta aggcggcacg gggagagtaa gctgatgagc aggaaactcc gctatggttt
       61 atcggctgcc gttctggcgc tgattgccgc aggtgcttct gcgcctgaaa tcctcgacca
      121 gtttctggat gaaaaggaag gcaaccacac cacggcatac cgtgatggtg cgggtatctg
      181 gaccatctgc cgtggagcca cccgggtgga tggtaagcct gttattcctg gcatgaagct
      241 gtcgaagggg aaatgcgacc aggttaacgc cattgagcgt gataaggcgc tggcatgggt
      301 ggagaaaaac atcaaagtgc cactgactga accccagaaa gcgggtattg cgtcattctg
      361 tccttacaac attggcccag gtaagtgttt cccgtcgacg ttttataaac gaattaatgc
      421 aggagatcga aaaggtgcct gcgaagcgat tcgctggtgg attaaggacg gtggcagaga
      481 ctgccgtatt cgctcaaata actgttatgg tcaggtatcc cgtcgtgacc aggagagcgc
      541 gctggcgtgc tggggaatcg acagataagc agaatatttt gctgaaaaat gacgttggcc
      601 agcgcgggcg gataacacga aatcctgcga actggcaaaa tgtaagtgaa taaagttagg
      661 cagattattt cacgcagagg caccgtaatg gtgcctttgt catttctgcg cttcgcacaa
      721 gcgtaaataa accaaagaac ctttcaggat gagccctggt ggataaccgg cagtggtctg
      781 gttaaccctc tttgggctgg ttattcctgt gcgcagggtt catcactaaa aggaatcaac
      841 catgaaagag atgatttctg tcgatcatga aatatccatg agtagtctgg attttctgaa
      901 taacattatt aatccagccc gggcagaagc cggagaagtc cctcatgaac cgcgtaagtt
      961 tcttgcaaaa attgaggatg agctagagct tgatggaacc ggaaaaaaat tccggttaaa
     1021 caataaccag acaagaacgg catactatga tctggatttt gaccagatga tgctcgttgg
     1081 catgagggag tcaaaggccg ttcgtcgttc tgtgctggca agactcaaag cgatgcatgg
     1141 tattcagatc ccccggactt tacctgaggc gttgcgattt gcggcaaaac tggctgaaca
     1201 gaaagcagtg ctggaaaatc aactggcaat agcagcgccg aaggctgaat ttgttgataa
     1261 ctatgttgaa gcatctggtc tgatgggatt ccgggaagtt gctaagttac tcggtatcaa
     1321 agaaaccgat ttccggctgt ttttgttgga gaacggaata atgtatcgcc tcgctggaaa
     1381 aatgacgcct tactcgcatc acctggatgc ggggcggttt agcgtgaaaa cgggcgaggc
     1441 gggcaatggt catgctttta cgcaggttaa attcacccca aaaggtgttc agtggattgc
     1501 tggtctgctt gctgcatgga gagctaccgc agcatgaaga tgataaaaat ggactggaaa
     1561 tttttgctgg tctggctgat tccgttttta tgggtggttg cccggttaat tactgctatt
     1621 aagaggtaaa gatgtcagac aaactcataa tgctggcgaa gggcctttgt gtaatcgtcg
     1681 gtatttcatt ttcactaatg ctggttgctc tttttctttc cataacctgg atggcgttga
     1741 cttcggcagg gctggtgggg tgagcataaa ccgaatgctt tccgcgttta ccgttattct
     1801 gctggtggtc tgtagtgcgc tgtggctggc aacagaccat taccgtgata acgccatcac
     1861 ctacaaagcg cagcgcgata aaaaagccag agagctggag ctggcaaacg caaccattac
     1921 tgacatgcag cagcgccagc gtgatgttgc tgcgcttgat gccagatact caagggaatt
     1981 agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg
     2041 cctgcggatc aacgccacct gctccggtac cgtgcgtgaa gccaccggca cctccggaat
     2101 ggataatgca accggccccc gactggcaga caccgctgaa cgggattatt tcaccctcag
     2161 agagcggttg atgacaatgc agaagcaact ggaaggggcg caggaatata tccgcactca
     2221 gtgcctgaat taacagagcc agcttaatcg ctggcttttt catatctgaa tttcatcgcg
     2281 catctcacgc gcatatcaca tccccgagcc tttcagaaag ttgagcctga gaactgtcgt
     2341 atatggtggc gaccatctcg gggcggcttt tctgtgagac aggctcaatt ttctgaaagg
     2401 taagacgcta tgaactatcc gacgattgtt aacggtattg atttccgcga tcttgttttt
     2461 gtgacaggca acgagccggt gacggatacc cgaaaggttg ccgtagcatt tggcaaagaa
     2521 cacaaagatg tattgcggaa aacaagagcc gttgttcaac aatgctcaaa agaatttgca
     2581 gagcgcaatt ttgcgctttg ctatgaaaac aatgagttac agaacggtaa accccagccc
     2641 atatatcgaa tgacaaaaga cggttggacg atgttggtat ttggttttac ggggaaagct
     2701 gctatgacct ttaaggaagc gtacattcag gctttcaact ggatggctga gcttattcag
     2761 caaggtctgg cgaatctgga ggcggagcgc aacgctgtaa tgctggagta catgaaagag
     2821 aaagatgtcg ccagtatgtc aggtcgtttg ctcaatcgct ggggcagggt gaagaagcct
     2881 cagttgctgg caaggctgga caggctggag cagcaggggc agattgcgtt acccggattt
     2941 gataaaggca tttcagcctg acatagccat gcgccgtatc gtcgccgtat cgtcgccgta
     3001 tccccgcatt aacagagacc gcagcccgac agggagactc ctctacgaga gtgtgcggga
     3061 taatcaaaaa cgatacacac cggggtttac cgcgttaacg gagcgcggcg ttgtccctca
     3121 tggtcgctgg tccggtgcga tggtggaaga aaccggacga tgtgttacct cgcaagctct
     3181 gtgatgtcat gtgtctgatt tgtgatttaa gtcggataat tgtcgttgcc attaagcaga
     3241 ggattgatga ccgacagggt ggcattgtta gaataagact tattcttatc tgtgccggga
     3301 atgaaaatga aaagaaatct tccgttaatt attttgttgt cttctctggt tatgggctgt
     3361 acgcaacata aaacagatat gccccgacag ttggttaagg cattaccaca atatccggcc
     3421 tatgcagcgg caaattatat aaagggacgg gttgatgtga agtttgatat tggtgctgat
     3481 ggtactgtca cccgaattga gtttatccgt tcagagccgc accatttgtt tgatgagcag
     3541 gttgtaaagg cgatggcaaa atggcgattt gagaaggaca ggccgtgtaa aggcgtgaag
     3601 aaaacgttta tctttagtcc ttctgcaccc tgattatttc atcagaaatt aattatcact
     3661 ctgttgttat tctgtacatc ccggcagggt aagtcttgtt ccgtcggata tgaagatgaa
     3721 atattgttgg aggacagtgg gtacctgctc ctgtaaccga acgttcattt ctcgttattt
     3781 gtcatgctgg ccgggcgcag atgcgttgca tctgttgcca gccttct
//
LOCUS       DAZFWP020000276         3773 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFWP020000276 DAZFWP020000000
VERSION     DAZFWP020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.278"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..1672)
                     /locus_tag="ACHK0Z_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /pseudo
     CDS             complement(1..1672)
                     /locus_tag="ACHK0Z_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1618..1839)
                     /locus_tag="ACHK0Z_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             complement(1618..1839)
                     /locus_tag="ACHK0Z_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504789.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(1884..>3773)
                     /locus_tag="ACHK0Z_RS24690"
                     /old_locus_tag="pgaptmp_004938"
     CDS             complement(1884..>3773)
                     /locus_tag="ACHK0Z_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4504790.1"
                     /translation="EDERVITGIASTPSPDRDGDILEPEGAEFGSTIPFLWQHDHSRP
                     VGQCTVRRVREGLEITAMLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHEY
                     TYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDHPFSAASGNRKPVVKIASSA
                     GAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRTLDVEEEEH
                     YDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGDVVTVASAPVIRVEQKLDKG
                     IGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGAGTTTDPQWAGSLSE
                     YQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWVGEGKARPL
                     TKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKK
                     AAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVTANLQPTGAVWLMSSTNALALS
                     MRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSR
                     EASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYG
                     TASGG"
BASE COUNT          766 a         1241 c          940 g          826 t
ORIGIN      
        1 ccgtccgccg gtaccggtat tttcccgacc gcatcattca ccgcctgctg cagtacatcc
       61 ggatcatagt cacgaccatc acgcggaaca gggatatggc ttgctgcgtc cttcaccatc
      121 tgctcaagca tcggacgcac atcatcaccc gtcacgcact tctgtaatac cacagactga
      181 gaagccagtt tttcttcaaa cgtttgtgcc tgcgcggcta ttttctcctc aaatgtacgc
      241 tgtaaatccg ccagcaccat ggcgaattct tcgcccagtg cacgaataat ggacagttcc
      301 cgttccgtca ttttcgcagt atccccctga acatcgcttt caccgcatca tgctctgttt
      361 cactgattgc cttattaccg tcagatgcgc catcaggcag ttgtgctgag actgttttcc
      421 cggacgacgc gaacggatcc tcacgggcat cacgacggga cagcgcctcc agactgtagt
      481 tctgctgctg aagatacagt gcatcacctc ccgcaagggg cggcaggttc tcacgtttac
      541 gggcctcatt gggcgtcagc agcgtatttt tcaccgactc acccagcgtt ttcatgcgcc
      601 gttcgctgtc cattctcagc agcgtggtga cgtcaaactc cgtactctcg ttttcccccg
      661 tttccagcgc ctcatccagt aacagctcaa tggactcaat cagcgtctgc agacactggg
      721 aataatactg ctgctccagc gcctccacgt tatcactgga gggaggttgt cccacaccaa
      781 tcttgtaggc cgggacacgg aacaccgaac agacaatttc agccgtcatt ttcagttgtt
      841 ccaccgtctg cgcatccacc ggtgaaaacg tcgtggggtt atatttcgcc ccgttgctca
      901 gaatcgccgt tttccccgca ttttcgccgg tatacccgct gtcccagttg ctcttcagtt
      961 ttttcgcatt ttcttccgta atactgccgg ggatctcaat caccccggac ggcctgccgc
     1021 catttctgaa aaaagacgtt gaatttgcct gaatatgatg cccctgcgtg gcagccagcc
     1081 cggcagcata caccggcggc agccccacaa gcggatgaaa aaaacagtta aaccggtcgt
     1141 ggatcacttc ccgggcaggc accgtcaccg cctccgtgat cccgcagttc cggtccggcg
     1201 tgatgcgata gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg ctccagtcca
     1261 gaatacgcag ttctttgatc tgcccacggg agttacggat tttcagcacc accgtattgc
     1321 cgtgacgcag tttggcgttc agccacagtt caaaaaactg gatacgattc tgctgtgcat
     1381 tgggacgacg acagagacgg gcaatatccc cctgccgttt ttcacggcgg atcccctgtg
     1441 tatcggtctg catcaggcgc agtcgcattt tggcgatatc ctgggatatc agcgaaatgc
     1501 aagaaaacac cgcatgaaag gagaggacac tttccggatc ggctttcact ccctgctgcc
     1561 aggcaccggc aaaaggctca gccaccgcct gaaacaggct ggtccagccc acctctttta
     1621 catcacgtcc tgatttctgg ttttttcggg gtcgccgcaa aaggttccac attcgccatg
     1681 ctccgcatca cgtttctttt tctgacctgc cggacgtcgc gccgtgatgt actccgcctt
     1741 ccccaggcga accagcacct ccgcacacgg ctgtgccaca tcacggatat ccccggcccg
     1801 ggcatcatgc gtgccctgca gatactggat ttttgccatc agttactgcg ggaagctcgc
     1861 gcctcccgcc ctcctcatca gactcagccg ccggacgcag ttccgtagtt cacaccggtg
     1921 atcaccgcca ccgccgcagt acggcgacga cgccagttga tccagcgctc cgcacggatg
     1981 gccacgctgc cggtctggaa catggagacc agctccaccg gtgacggtgt gctgctgtcg
     2041 ctggtcggtt cagactgcat ctccagtgac gcttcacggg acatatccac cgccacacca
     2101 ccgtcatcag ccagataaat atccggcgca ttcaccagta ccagctggtc acccacatac
     2161 tgggagacaa tcaccggcag cccctggaag gtcccgccca gcagggtcat gtccggatat
     2221 tccttctgcc ccagcgcatt tttacgcatg gacagcgcca gggcattcgt gctggacatc
     2281 agccagaccg caccggtggg ctgcagattt gccgtcacaa actggccaaa cgcggcctcg
     2341 gcatccgcat ccggattacc gcttgacgcc gtgcccttca catcatgggt gatggacgca
     2401 ggggagacat ccgccaccgc cgcttttttc ggatcaacaa agtctgtgtc cagacgtgcc
     2461 accaccgcct ctgccagcgc attacggacc agtgcatcag cagccgggct ggaaaaacgg
     2521 atcagctctt ccgtcagtac cgcaatggcc gacaccttcg catgactgaa ggtaatggat
     2581 tcaaaatcaa acttcgtcag cggtctggcc ttgccctcac ccacccagcc tgcagctccg
     2641 ccggacacct gggcatgcac acggatattg aacggaacct gacgaagggc cgggatcccc
     2701 ccctgaccaa atcgcccgat aatggtctgc ggacgcaggt aatcaataaa gtcctgcgca
     2761 tattcctggt attcagacag gctgcctgcc cactgtggat ctgtggtggt ccccgccccc
     2821 accgccgatt tcagaacatg atgcagacgg ctgtcatccg gatactgacg gcgggccact
     2881 tccagggctt cggagcggac acctttagcc gcggccagcg atttggcaaa acgtgcgaaa
     2941 ccaatcccct tatccagttt ctgctccaca cggatcaccg gcgcagaggc caccgtcacc
     3001 acgtcaccgt taccagcctg tttcaccggc tgtgccgtgg cggccttacc ggcttccagt
     3061 tcacgcaggc gcttcaggtg agcatccacc tgacggattt ccgctgcggt gttgtcgtag
     3121 tgctcttcct cctccacatc cagcgtgcgc ccttcctctg cggctttggt catgacctcc
     3181 tcaagggagg ctgccagcgc tgcacgcttg ttttcaaaac ttttaatctg ttcgccaata
     3241 ttcattatgg tcttttcctt atgaaaaacg gttgttgact gtgccgcagc gccggcagaa
     3301 gatgcgattt tcaccaccgg tttccggttg ccggacgcgg cagaaaacgg gtggtcgtaa
     3361 gatttaatgg tccggatggt gcattccgca ttcgcgggca cggtgacggc agacacctcc
     3421 atcagttccc agcgcagaaa atgcagtccg cctccgtcca gataagtgta ttcatgggga
     3481 cggaagccca cggacagccc cctgaccagc ccggtcttaa tggccgccca gacctcatcc
     3541 agccgggcag ccagttgcga cggcatatcc ggtacgggct tcaccagcat tgccgtgatt
     3601 tccagccctt ccctgacccg acgcaccgta cactggccta cagggcggga atggtcatgc
     3661 tgccagagaa acgggatcgt actgccaaac tccgccccct ccggctccag gatgtcacca
     3721 tcccgatccg gagaaggcgt tgacgcaatc ccggtgatca cccgttcatc ctc
//
LOCUS       DAZFWP020000277         3767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFWP020000277 DAZFWP020000000
VERSION     DAZFWP020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.279"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(147..491)
                     /locus_tag="ACHK0Z_RS24695"
                     /old_locus_tag="pgaptmp_004939"
     CDS             complement(147..491)
                     /locus_tag="ACHK0Z_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504791.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFNKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(520..687)
                     /locus_tag="ACHK0Z_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             complement(520..687)
                     /locus_tag="ACHK0Z_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504792.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(723..1022)
                     /locus_tag="ACHK0Z_RS24705"
                     /old_locus_tag="pgaptmp_004941"
     CDS             complement(723..1022)
                     /locus_tag="ACHK0Z_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504793.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(1180..1458)
                     /locus_tag="ACHK0Z_RS24710"
                     /old_locus_tag="pgaptmp_004942"
     CDS             complement(1180..1458)
                     /locus_tag="ACHK0Z_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU4504794.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(1458..2141)
                     /locus_tag="ACHK0Z_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             complement(1458..2141)
                     /locus_tag="ACHK0Z_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HGU4504795.1"
                     /translation="MASLERELIRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEP
                     DDLSEWADMHFLLWDAQRRAGISDAEITAAMEDKLKINMERQWPEPKDGEPRLHIKEP
                     GNSPVIPDGLSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSL
                     PVTPDGWISCSELMPDKLIPVMVMYEDGEMWSAMWNGNRWDDGTEYPDPHSVTHWREM
                     PAAPQQEVK"
     gene            complement(2115..2240)
                     /locus_tag="ACHK0Z_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /pseudo
     CDS             complement(2115..2240)
                     /locus_tag="ACHK0Z_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            complement(2251..3006)
                     /locus_tag="ACHK0Z_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             complement(2251..3006)
                     /locus_tag="ACHK0Z_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504796.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHVNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAL"
     gene            complement(3008..3415)
                     /locus_tag="ACHK0Z_RS24730"
                     /old_locus_tag="pgaptmp_004946"
     CDS             complement(3008..3415)
                     /locus_tag="ACHK0Z_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4504797.1"
                     /translation="MSEINSQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            complement(3412..3633)
                     /locus_tag="ACHK0Z_RS24735"
                     /old_locus_tag="pgaptmp_004947"
     CDS             complement(3412..3633)
                     /locus_tag="ACHK0Z_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4504798.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT          922 a          959 c          802 g         1084 t
ORIGIN      
        1 gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca cgcaagaaaa
       61 agccgcattg atgcggcgat ggtaggtctg gatatcattg agcaatgaac aggcctcacc
      121 gagtgtgagg cggtgttatt tcatggttag tccttgcgta gctcgctgat tcttctgtaa
      181 gtctctggtg ctttgtttcc gtgtatcttc atttcagact tcaacagagc aacgagggaa
      241 tcccattcgt tgaggattcc tttgaatgcc ggaacgcgct ttgcaacctt gttgaatgaa
      301 tctctgattt ctggaatctg ctcaacaagt gcaacgcatc gtctgaaatc ggctgcgtca
      361 tggggagcgc cgaagtgatg accatagata ttctttttca gtccacatgc gattgaggca
      421 agagttgcgc tactgatgcc aacatcgcca gtcgattgcc atttcaaaac cttcatagcc
      481 aaatctgaca tttcttgtct ccaataaaaa aaaccgccat caggcggctt ggtgttcttt
      541 cagttcttca attcgaatat tggttacgtc tgcatgtgct atctgcgccc atatcatcca
      601 gtggtcatag cagtcgttga tgtcctctgc ttcgataact ctgttgaatg gttctccatt
      661 caattcacct gtgactcgga agtgcatttg tgatttcccc aaaagatgct tgagtgcgct
      721 tcttattcga ttcgcacacc tggtatttcg ccttttgaaa tggctaagtc ataaatttgc
      781 gcagcactat acccatctcg catccatgaa tctaaggcgc gaatagcctc gctacgcttt
      841 ttatcttctc tttcattttt gatatcaacg aggacatcaa cgcaattaag gcatatgtgg
      901 attttgtcct tacattcgat catggcagct ttaccatgat ttccgccaca cagtgagcat
      961 aaatcttcag ggtctggctg gtatttctgt aacgttaaag ggttgaatgt tgaacaggcc
     1021 ataatcatct ccataaaaca aaactcgccg tagcgagttc agataaaaga aatccccgtc
     1081 agtgcgagga ttgttattgt cttttcttcg tgcatttgtc gcactttcgg caccatccag
     1141 ataggcacat ccgtccgcaa ttaacacata taggccacat catttttcct cttttggttt
     1201 atgaatctga atggtcatgc cgctttgagt ggtgactaca atgacataac caggctgaag
     1261 actgttaaga ttgaatgctt cgtaaaacga atccaaggcc agcgcttttt tattctttcg
     1321 gttccaccaa cgccatccct tgctacaggc tacactgaca atccactgcc cactcctgta
     1381 agccatataa aaccagatga gcaaaacctg aaggaatgct atccagtcaa taatcgtata
     1441 tttcgcgaag gagtccatca cttcacctcc tgctgcggtg ctgctggcat ttcacgccag
     1501 tgcgtaactg agtgcggatc cggatattcg gtgccatcat cccagcgatt gccattccac
     1561 attgcagacc acatctcacc gtcttcatac atgaccatta ccggaattaa cttatccggc
     1621 attagctcac tacagcttat ccaaccatcc ggagttaccg gaagcgagaa cggcagcaca
     1681 tctctgtgaa caagtttttg ctgtgacagg ttatccagaa ctttctgtac tgctgcatca
     1741 ccgaatacac caagcgcatc tgccataact cctacaacct gataagcctc agcgcatacc
     1801 gtggataaac catccggaat taccggagag ttgccgggtt ctttaatgtg caagcgaggc
     1861 tcaccatctt ttggttcagg ccactggcgc tccatgttga tcttcaattt atcttccata
     1921 gcagcggtaa tttcagcatc gctgatgcca gcacggcgct gtgcatccca caacaggaaa
     1981 tgcatatcag cccactcgct gagatcgtct ggttcggctg cggcttccag agcctctttt
     2041 gagaggtgtt tcagtggacc aatggggcca acgcagccaa atgtggagtc agaccatttg
     2101 gcatgctcgt ggcgaatcag ttcgcgttcc agtgatgcca gtgcaattcg tgccagttcc
     2161 atttgttcgc cacgagtaag cccgttttca agcggatttt taatgaacaa ttcaatacgt
     2221 tctttggtaa tagtggtcat ttgttagtcc ttaaagtgct agttgcaatt gcatttcaaa
     2281 gcggtcgcgt tgttcacaat acgcaagaga accagggcta ttgtgtgcct caatccgttc
     2341 taccattaat gctgcgcgtg tctctttact tgcaggtgca taagccccag accaggcttt
     2401 atcaataccg atgtttcgag cgacgttcgt actatctgcg ctggctaagg gtaattttgt
     2461 gaatatcagc ggatttaaca tgcgcaatcc atgtagtttc gtaaccggct gaccatgccc
     2521 atcaacaatg tgacgaatca ggtctttcat tctggctacc gcaagagttg ggcgctttac
     2581 gtcatagtcg ccacaactac cgatagccac tcgcggaaac tcattgcaca aatgaataaa
     2641 tcgctcgtca ctttcattca cgtgccacac tggaacgcca gctagttttc cgtgaggcca
     2701 ctcattcaga agcgcatcat tttcctcctc tccgccatca ataacatccg ggataatggc
     2761 aaaatcgaat cctgggtgat tcttccagcg agcaacaaac tcgtagtaat cgctccagtc
     2821 gattttgttt ttgccagctg ctttccaggc ggtgaatgca ccgttgtcca gcgcgaacga
     2881 ctgacagtat tcagccgcga gattgatctg gcctgaatgc gcaaaactga taaacgcatg
     2941 tcgccctttc catgctctca ttgcgcacgt atcaggagta ataggcccac cgtggtagtg
     3001 aatcatctca ctctcctttg atgcgaatgc cagtagcgcg gattgcatcg atgacttcag
     3061 aaactttgta tgccattacc gtttggtaat catcgtgaaa atctgttcga tgaagcatgc
     3121 tgctacgttc cgggagcagt atttcccgcg cttccagttc tgcaatgcgc ttttttgctg
     3181 cttccagttc atccagtaat tccagcacgg tagccggatt agccttggca acaaaatccc
     3241 ggactggctt acaatcaatc tccgcaatgg gttgatacga tgtgtagcca tgctgtcttg
     3301 tataactacc gtgacgaata acgaaaaaat caccatttat ttttttagcc tgccacttat
     3361 cttcaccggc tttctctgcc gcttcacgca gtgcctgaga gttaatttcg ctcacttcga
     3421 acctctctgt ttactgataa gctccagatc ctcctggcaa cttgcacaag tccgacaacc
     3481 ctgaacgacc aggcgtcttc gttcatctat gggatcgcca cactcacaac aatgagtggc
     3541 ggatatagtc tggtagttca gacgacgcat ttttattgct gtattgcgct gtaattcttc
     3601 aatttctgat gctgaatcaa tgatgtccgc catctttcat taatccctga attgttggtt
     3661 aatacgcttg agggtaaatg cgaataataa aaaaggagcc tgtagctccc tgatgatttt
     3721 gcttttcatg ttcaccgttc cttaaaaacg ccgtttaaca tgccgat
//
LOCUS       DAZFWP020000278         3763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFWP020000278 DAZFWP020000000
VERSION     DAZFWP020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.280"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            133..579
                     /gene="hpaR"
                     /locus_tag="ACHK0Z_RS24740"
                     /old_locus_tag="pgaptmp_004948"
     CDS             133..579
                     /gene="hpaR"
                     /locus_tag="ACHK0Z_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HGU4504799.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            698..2362
                     /gene="tsr"
                     /locus_tag="ACHK0Z_RS24745"
                     /old_locus_tag="pgaptmp_004949"
     CDS             698..2362
                     /gene="tsr"
                     /locus_tag="ACHK0Z_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4504800.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2411..>3763)
                     /locus_tag="ACHK0Z_RS24750"
                     /old_locus_tag="pgaptmp_004950"
     CDS             complement(2411..>3763)
                     /locus_tag="ACHK0Z_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4504801.1"
                     /translation="ENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAMLLL
                     FFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLDRKG
                     PRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFNIKE
                     RGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYRNRE
                     HVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLYLAW
                     LPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKICIIA
                     GMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASVGSI
                     QNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDPRKD
                     "
BASE COUNT          992 a          914 c         1037 g          820 t
ORIGIN      
        1 ttaaatatca gcaagttaac aaaacatgct caattttgat aaaatgaaga attgcagggg
       61 cagcgcagtc ccggtaacgt gatgattaac tgtttctata ttaatgattt tgaatttttc
      121 aggggaaaga taatgcacga ctctctaacc attgcgttgc tccaggcgcg agaagcggcg
      181 atgagttatt ttcgcccaat cgttaaacgg cataatttga ctgagcaaca gtggcgcatc
      241 gtgcgtattc tggcggagag cccatcaatg gattttcacg atctggcgta tcgcgcctgc
      301 attttgcgcc caagcctgac cgggatcctt acgcggatgg aacgcgacgg tttagtgttg
      361 cgattaaagc cgatcaatga tcagcggaag ctctatatct cgctgaccaa agaggggcag
      421 gcgctgtata accgcgctca gacacagatt gaagaagcct atcggcagat tgaagctcag
      481 ttcactgccg agaaaatgca acagttaacc catttgttag aggaatttat tgctctgggc
      541 aattcccgac aagaagacat cccgggcgat aatgaataac tttttagtat ctgataacca
      601 gcgcctggtt tttccatgga tggcgggtta caccttttca taaagttttt gctttccagg
      661 ccgaaaatct tgcatcggtc cacaggaaag agaaaccatg ttaaaacgta tcaagatcgt
      721 gaccagctta ctgctggttt tggccgtttt tggcctttta caactgacat caggcggtct
      781 gttctttaat gccttaaaga atgacaaaga aaatttcact gttttacaaa ccattcgcca
      841 gcagcaatcc acgctgaatg gcagctgggt cgcgttgttg cagacgcgta acaccctcaa
      901 ccgcgcgggt atccgctaca tgatggatca gaataatatt ggtagcggtt caaccgttgc
      961 tgagctgatg cagagtgcca gtatttcgct gaaacaggcg gaaaaaaact gggcggatta
     1021 cgaagcgttg ccgcgtgacc cgcgtcagag caccgccgca gcggcagaga tcaaacgtaa
     1081 ttacgatatt tatcacaatg cgctggcgga gctgatccaa ctgttaggtg caggcaaaat
     1141 caacgagttc tttgatcagc cgactcaggg atatcaggac ggtttcgaga agcagtatgt
     1201 ggcttacatg gagcaaaacg atcggctcta tgatatcgcc gtcagcgata acaatgcctc
     1261 ctacagccag gcgatgtgga ttctggtggg cgtgatgatc gtcgtactgg cggtcatctt
     1321 cgccgtctgg ttcggtatta aagcctcgct ggtagcgcca atgaatcgcc tgattgacag
     1381 cattcgtcat attgcaggcg gcgatctggt gaaaccgatt gaggtggatg gctctaatga
     1441 gatggggcaa ctggcagaga gtttgcgcca tatgcaggga gagctgatgc gtaccgtcgg
     1501 tgatgtgcgc aacggggcca atgccatcta tagcggtgcc agcgaaatcg ctaccggcaa
     1561 taacgatctc tcttcgcgca ccgagcaaca ggccgcttcg ctggaagaga cggcagccag
     1621 catggagcaa ctgaccgcaa cggtgaaaca gaacgccgag aatgcgcgcc aggccagcca
     1681 tctggcatta agtgcttctg aaacggcgca acggggcggc aaagtggtag ataacgtggt
     1741 gcagactatg cgcgatatct ccaccagttc gcagaaaatc gccgatatta tcagcgtaat
     1801 tgacggcatt gccttccaga ccaatattct ggctttgaac gcggcggttg aagcagcgcg
     1861 tgcgggtgag caagggcgcg gttttgcggt ggttgcggga gaagtgcgta atctggccca
     1921 gcgtagcgct caggcggctc gtgaaattaa aagcctgatt gaagactcgg tggggaaagt
     1981 ggatgttggc tctacgctgg tcgaaagcgc cggggaaaca atggcggaga ttgtcagcgc
     2041 cgtgacccgc gtgacggaca ttatgggcga aattgcttct gcttctgatg agcagagccg
     2101 tggtatcgat caggttggct tagcggttgc tgagatggac cgggtaactc aacagaacgc
     2161 cgcgctggtg gaagaatctg ccgctgccgc cgccgcgctg gaagagcagg ccagtcgcct
     2221 gaccgaagct gtggcagtgt tccggattca gcaacagcag caacagcagc gtgaaacatc
     2281 ggctgtggta aaaaccgtga cgccagctgc gccgcgtaaa atggccgtgg cagatagcga
     2341 ggagaactgg gaaacatttt aatcgccatg aaaatgcccg ataagcaaaa tgttatcggg
     2401 cataaggaga ttaatcttta cgtgggtcgt tgatcggctg acgaaccagg aagatgtacg
     2461 ccagtgcccc cgctgcggtg acgcaaccgc agatgattag tgccagacgg aatgagtggg
     2521 tggtatcaac aataaaacca gtaatgatcg gcgcaaaaga ggcgcagatg aagctggcaa
     2581 agttctggat actgcccacc gacgcagtca tgcgagaagc aactgcgacg tggatcaagc
     2641 cccagcagga tgttccggca aagtgaatac agaacagtgc catgccaatc agcagaaccg
     2701 ccgtcatgga tgttgtcgct tgtggtacta tcagcgtaaa ggcggcagaa cagaacatcc
     2761 cggcaataat gcagatctta cggcttttaa tcggagccat tccccctttg accagccagt
     2821 cagtaacgta accgttgacc agcatcccgg cagccccaaa caggaaaggg atagccgcca
     2881 tcaaccctgt gctttttaaa tccaggttat aggctgtttg caggtaacca ggaagccagg
     2941 ccagatacag ccacgcagtg tagttgatgc cactgaatcc gagcatcatt ccccacattg
     3001 tacggttacg gaacaggctg cgccattcgg caaaactgag cggatctcgg cgggcattga
     3061 cgctacctgc attgagataa gcttgttcaa cggcagtcag ttctacgtgc tcgcggttgc
     3121 gatagagcat ataccagccg atggcgagaa aaatccccag tacaccaatg gtaataaaca
     3181 tcccgcgcca gcccatcacc agcatcatcg ccgccagaat cggtgggctt acggcaacgc
     3241 caatggtaga agctgcgttg aagaagccca tcgggcgtcc gcgctctttg atgttgaacc
     3301 agtcgttaat gacttttaca ccgcatgggt tcatcggcgc ttcaccaatc cccataccga
     3361 tacgcaccaa cacgaactgc gtaaagttgt gcaccatgcc agacattgcc tggaacagtg
     3421 accagaagaa catccccagt cccagcatca ggcgtgggcc tttacgatcc aatagtgggc
     3481 cgcaaggaag ttgcgcaatc ccgtaagcga gtgaaaacac ggagagcaaa gcgccgattt
     3541 cggtggcact taatcccaat tcttcacgaa tcgttaaatt tgctaccgac agcgaactgc
     3601 ggtcgagata attgattacc gccgcaaaaa ataataacaa catggcggtg gtttgaatgc
     3661 gtttaattcg ggtactgcgt tgaactaatc catctggtgg cacgggaata tctgccgacg
     3721 aagatggagt aaatgaagaa cgcggatcga gggtgatatt ttc
//
LOCUS       DAZFWP020000279         3705 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFWP020000279 DAZFWP020000000
VERSION     DAZFWP020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.281"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHK0Z_RS24755"
                     /old_locus_tag="pgaptmp_004951"
     CDS             complement(<1..245)
                     /locus_tag="ACHK0Z_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4504802.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQ"
     gene            343..1035
                     /locus_tag="ACHK0Z_RS24760"
                     /old_locus_tag="pgaptmp_004952"
     CDS             343..1035
                     /locus_tag="ACHK0Z_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4504803.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(1355..1870)
                     /locus_tag="ACHK0Z_RS24765"
                     /old_locus_tag="pgaptmp_004953"
     CDS             complement(1355..1870)
                     /locus_tag="ACHK0Z_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504804.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            2015..2326
                     /locus_tag="ACHK0Z_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /pseudo
     CDS             2015..2326
                     /locus_tag="ACHK0Z_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2341..2703
                     /gene="yfdP"
                     /locus_tag="ACHK0Z_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     CDS             2341..2703
                     /gene="yfdP"
                     /locus_tag="ACHK0Z_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4504805.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            2769..3593
                     /locus_tag="ACHK0Z_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             2769..3593
                     /locus_tag="ACHK0Z_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU4504806.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         1041 a          836 c          836 g          992 t
ORIGIN      
        1 tcctgttcct gtggtttaaa cccattctgg ttttggctag attgaaaacg tgccggataa
       61 agaatctgca tttcgctgac ttcaccctta aaaaaattgg ctaaacgttc tgcaagctcg
      121 atagatggaa tctgctccag cctctcaata cgactcaacg tcgctggatt gacttgaaca
      181 cccgcagcaa catgctgcaa agtgaaacca tgcgccttac gcacatttcg taatggtgat
      241 tgcatacgtc ctccaaatat tgcgcgttat gcatgttatt tcacgcaatt attttgcgca
      301 agttgatttg cttatcacgc aataaagaaa tgtaataaac gcatgaacat aggaaaccga
      361 gtcagacaac ttcgccaagc gaagaacatg aaaatcgccg atctcgctga agcgatagga
      421 gtagatgcgg cgaacatctc gcgcttagaa acgggtaagc aaaaacaatt taccgaacaa
      481 acactgagta atattgccaa gagcttaggt gttgatattg ctgatctctt tacctctgcc
      541 cacaaaagta atactgtata taaaaacagt aataatgagg atgttgcgca ggtgaaggat
      601 gtgttccgta ttgaaatgct ggatatcagt gccagtgcgg gaaatggcct tatccagggc
      661 ggtgatgtca ttgatgtgat tcatgccatc gaatacagaa ctgataatgc tgtatcaatg
      721 ttcggcggac gaccagccaa tcacatcaaa gttatcaacg ttcgtgggga cagtatgtgt
      781 ccaaccattg agccaggaga tctcatcttc gttgatgtca gcatcaatca gtttgatggt
      841 gatggtatat atgtctttgg ttttgatgac aaaatatacg ttaaaagact tcaaatgatt
      901 cctgacaaac tgctggtgat ttctgataac cagatttacc gtgaatgggg aattactagc
      961 gaaaacgaac accgattcat ggtctttgga aaggtcttaa tcagtcagtc gcaaaccctt
     1021 aagagacata attaacctca atatcccatc catcggccac cgaaaggtgg ctttttatta
     1081 cctataaatt tgcatatcac gcaaatatca cttgcatatc tcgcaattta attttatctt
     1141 ttgttccaga ccaactacag gattacaaca aaatctggtt gcaacacggt gcatgtgtcg
     1201 taagcagtca gtaaatgtca aaaacgaaca ggcaggacgc ccacgaagta gccgcctggg
     1261 gcatatgaag tccaggatga ttcgttagca acaaaaaagc gccctacagg acgcttagct
     1321 ctttaacaat ctggatatcc acaacagtag taatctacag attgccgtta agttttctgg
     1381 acaactcctc aatggatgga ggcgatacgt aatccggatt tttattcatc agaaacttat
     1441 tttcacagtg gaggcacctg cttttatgaa aaagctcatc ttcgctaacc gggaatggtt
     1501 gaagtatcga tactatcttt tgtccaaaac attttgggca aagatgcatg gttatgctgc
     1561 caccgttcac gattacctcc ttcgagtata caaaagtacc cgactcaagt tggttaagga
     1621 tatagccttc cgtctgagcc tcaaagtttt cgaattctgc aattttagct ttgagagaag
     1681 catttatttc ttgataagag cccaccagtt caacgagaga cacgcattcg cgctgaatag
     1741 acgcaagctt tgagttcagc tcaccaatag ccgcatttac ttcagcttga gtttttgcct
     1801 cgttcattag ttttgcaatc tgggctgttt cacgaatagc cgtcattgct gccgttaatt
     1861 cagcgatcac attgaatact cttattgttg ttggggatat ccagattaac cgaatccttg
     1921 ttgttgggga ataaccaggt ccacctcgcc tgatgtggct aaaagcaggc acataacagc
     1981 taagtatttt caaccagaga gaatccttag cgttgtggtg aatgcggctc agcgcacgcg
     2041 ggttaaggtt gaggctgaca gtcgaccttc tgtggatacc cacccgcctg gtgtgcaacc
     2101 ttcgccaggc accgggaggc acccggcacc acaactttat gctgtgtgta gtcctggcgg
     2161 taccagtttg tacccttgct tccggctggt accgtccttt tttgcaaaac agagaagagc
     2221 atcaccggac gacgggctca taacccaatc catccgggcg gctgccaccg caggtgttct
     2281 tctctgtttt gtggagaaac caaccgacct tgcaaggtcg atatgatgag gagcagcaaa
     2341 atggctagcg aacgcagtac tgatgtgcag gcatttatcg gggagctgga cggcggcgta
     2401 tttgaaacca aaatcggcgc agttctcagt gaggtcgctt ccggtgtgat gaacacgaaa
     2461 accaaaggga aggtctcact caatctggaa atcgaaccgt ttgatgagaa ccgtgtgaaa
     2521 atcaaacaca aactctcata tgttcgccca actaaccgcg ggaaaatttc cgaagaagac
     2581 accaccgaaa cgccgatgta tgtcaatcgc ggtggtcgcc tgactattct gcaggaagac
     2641 cagggacaat tactgactct tgccggtgaa cctgacggaa aactccgcgc agcaggtcgt
     2701 taatatcgtt cgtaataaac tgattattta tctcatcact gaatatcttt atatagtgag
     2761 gacttattat gtctcagaac ttagacgcaa ccgcaattaa tcaaatccat gcccttattt
     2821 ctgctcaggg tgttaatgaa attatcagta agattggtgc cgatgctgtg gcattgcctg
     2881 agaatttccg cattcatgat ctggaaaaat ttaatttaaa tcgcttccgt ttccgtggtg
     2941 cactttccac tgccagcatc gatgatttta cccgttattc taaagatctt gcagatgaag
     3001 gcacccgctg ctttatcgat gccgataata tgcgtgccgt cagtgtgctt aacctgggta
     3061 ctattgatga gccaggtcac gcagataaca ctgccactct caaactgaaa aagacagcac
     3121 cgttctctgc tctgttgtct gttaacggcg agcgtaactc ccagaagtca ctggcagaat
     3181 ggatcgaaga ctgggccgac taccttgtgg gctttgatgc taatggtgac gccattcaag
     3241 caacaaaagc ggctgcggca gtccgtaaaa tcacgattga agcaaaccag accgctgatt
     3301 ttgaagataa tgacttcagc ggcaaacgct ccctgatgga gtctgtcgaa gcgaagacca
     3361 aagacattat gccagtggca tttgaattta aatgcgttcc gtttgaaggt ctgaaagaac
     3421 gtccgtttaa attacgcctc agcattatca ctggcgatcg tcctgtactg gttctgcgca
     3481 ttattcagct ggaagcggtg caggaagaaa tggctaacga atttcgtgat ctgcttgttg
     3541 agaaattcaa agacagcaaa gtagaaacct ttattggtac tttcaccgcc tgatttcatt
     3601 actgcaaatg cccctgcggg ggcatttatg gaaacgtaat taactcaata atcaccggat
     3661 ggtgagggct tccttttacc agaattcagc gcggtgcagt gcata
//
LOCUS       DAZFWP020000280         3508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFWP020000280 DAZFWP020000000
VERSION     DAZFWP020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.282"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..147
                     /locus_tag="ACHK0Z_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             <1..147
                     /locus_tag="ACHK0Z_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HGU4504807.1"
                     /translation="FVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIF
                     LSRK"
     gene            422..1618
                     /gene="kbl"
                     /locus_tag="ACHK0Z_RS24790"
                     /old_locus_tag="pgaptmp_004958"
     CDS             422..1618
                     /gene="kbl"
                     /locus_tag="ACHK0Z_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU4504808.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1628..2653
                     /gene="tdh"
                     /locus_tag="ACHK0Z_RS24795"
                     /old_locus_tag="pgaptmp_004959"
     CDS             1628..2653
                     /gene="tdh"
                     /locus_tag="ACHK0Z_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU4504809.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            2892..>3508
                     /gene="waaH"
                     /locus_tag="ACHK0Z_RS24800"
                     /old_locus_tag="pgaptmp_004960"
     CDS             2892..>3508
                     /gene="waaH"
                     /locus_tag="ACHK0Z_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4504810.1"
                     /translation="MMNSTSKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRAR"
BASE COUNT          825 a          802 c          950 g          931 t
ORIGIN      
        1 tttgtcatgt gctattacaa acgccccgat ctcttcaacc taaactatct ggggagagga
       61 aaatggttcg atttgtttcg ctgcttcagg agtaacactt taggggcaaa aatgcaggcg
      121 ctgagaattt tcctttcaag aaaataggtt tccctccatt agtgggtagc gttttgatac
      181 caactgagat aggaaaaatg atgctttggc atcatttttt ctttttatat caatgcagta
      241 aaatcaatca tcattatgcc cagccaaaac tgtacagttt ttctactatc tgtaggtgtt
      301 gagagacatc aagttacacg ttatttatcc tgaattttgc agaagtgtta acgcgtcatc
      361 tcgtcgcgac ctataagttt gggtaatatg tgctggaatt tgccctgtct ggagaatcgc
      421 aatgcgtgga gaattttatc agcagttaac caacgatctg gaaaccgcac gggcggaagg
      481 gttgtttaaa gaagagcgca ttattacgtc tgcgcagcaa gcagatatca ctgtggctga
      541 tggaagccac gtcattaact tttgtgccaa caactatctc gggctggcga atcatcctga
      601 tctgattgcg gcggcaaagg cgggaatgga ttctcacggt ttcggcatgg cttcggtgcg
      661 ttttatttgc ggcactcagg acagccataa agagcttgaa caaaaactgg cggccttcct
      721 ggggatggaa gatgcgattc tctactcttc ctgctttgat gctaacggtg gcctgtttga
      781 aacgctgctg ggcgcggaag acgccattat ctccgacgca ctgaaccacg cgtctattat
      841 tgatggtgtg cgtctgtgta aagctaaacg ctatcgctat gccaacaacg atatgcagga
      901 gcttgaagct cgtctgaaag aagcgcgtga agccggtgcg cgtcatgtgc tgattgccac
      961 cgatggtgta ttctcgatgg acggcgtgat tgccaatctg aagggcgttt gcgatctggc
     1021 agataaatac gatgccctgg tgatggtaga cgactcccac gcggtcggtt ttgtcggtga
     1081 aaatggtcgt ggttcccatg aatactgcga tgtgatgggc cgggtcgata ttatcaccgg
     1141 tacgcttggt aaagcgctgg gcggggcttc tggtggttat accgcggcgc gcaaagaggt
     1201 ggttgagtgg ctgcgccagc gttctcgtcc gtacctgttc tccaactcgc tggcaccggc
     1261 cattgttgcc gcgtccatca aagtactgga gatggtcgaa gcgggcagcg aactgcgtga
     1321 ccgtctgtgg gcgaacgcgc gccagttccg tgagcaaatg tcggcggcgg gctttaccct
     1381 ggcgggagcc gatcatgcca ttattccggt catgcttggc gatgcagtag tggcgcagaa
     1441 atttgcccgt gagctgcaaa aagagggcat ttacgtcacc ggtttcttct atccggtcgt
     1501 tccgaaaggt caggcgcgta ttcgtaccca gatgtctgcg gcgcataccc ctgagcaaat
     1561 tacgcgtgca gtagaagcat tcacgcgtat tggtaaacaa ctgggcgtta tcgcctgagg
     1621 atgtgagatg aaagcgttat ccaaactgaa agcggaagag ggcatctgga tgaccgacgt
     1681 tcctgtaccg gaactcgggc ataacgatct gctgattaaa atccgtaaaa cagccatctg
     1741 cgggactgac gttcacatct ataactggga tgagtggtcg caaaaaacca tcccggtgcc
     1801 gatggtcgta ggccatgaat atgtcggtga agtggtaggt attggtcagg aagtgaaagg
     1861 cttcaaaatc ggcgatcgcg tttctggcga aggccatatc acctgcggtc actgtcgcaa
     1921 ctgtcgtggc ggtcgtaccc atctgtgccg caacacgatc ggcgtcggcg ttaaccgtcc
     1981 gggctgcttc gccgaatatc tggtgatccc ggcgttcaac gccttcaaaa tcccagacaa
     2041 tatctccgac gacctggctt ccatttttga tcccttcggt aacgccgtgc ataccgcgct
     2101 gtcgttcgat ctggtgggcg aagatgtgct ggtttctggt gcaggtccga taggtattat
     2161 ggctgcggcg gtggcgaaac acgttggtgc acgcaatgtg gtgatcaccg atgttaacga
     2221 ataccgcctt gagctggcgc gcaaaatggg tatcacccgt gcggttaacg tcgcgaaaga
     2281 aaaccttaat gatgtgatgg cagaactggg catgaccgaa ggctttgatg tcggtctgga
     2341 aatgtccggt gcgccgccag cgtttcgtac catgcttgac accatgaacc acggcggccg
     2401 tattgcgatg ctgggtattc cgccgtctga tatgtctatc gactggacca aagtgatctt
     2461 taaaggcttg ttcattaaag gtatttacgg tcgtgagatg tttgaaacct ggtacaagat
     2521 ggcggcgctg attcagtctg gcctcgatct ctcgccgatc attacccatc gtttctctat
     2581 tgatgatttc cagaagggct ttgacgctat gcgttcgggc cagtccggga aagtaattct
     2641 gagctgggat taacacgaac aagggctggt attccagccc ttttgcctga ggataatccg
     2701 ttaaatatgt aaaatcctgt cagtgtaata aagagttcgt aattgcgctg atctcttata
     2761 tagctgttct cattatctct ctaccctgaa gtgactctct cacctgtaaa aataatatct
     2821 cacaggctta atagtttctt aatacaaagc ctgtaaaacg tcaggataac ttcagaggtc
     2881 gtcggtaatt tatgatgaac agcaccagta aacttagtgt tattattccg ttatataatg
     2941 cgggcgatga tttccgcact tgtatggaat ctttaattac gcaaacctgg actgctctgg
     3001 aaatcattat tattaacgat ggttcaacgg ataattctgt tgaaatagca aagcattacg
     3061 cagaaaacta tccgcacgtt cgtttgttgc atcaggcgaa tgctggcgca tcggtagcgc
     3121 gtaatcgtgg gatcgaggtg gcgacgggca aatatgtcgc ttttgtcgat gctgacgatg
     3181 aggtctatcc caccatgtac gaaacgctga tgaccatggc gttagaggac gacctcgacg
     3241 tggcgcagtg caacgctgac tggtgttttc gtgaaacggg agaaacctgg caatccatcc
     3301 ccagcgatcg ccttcgctca accggtgtat taaccggccc ggactggctg cggatggggc
     3361 tttcttcgcg ccgttggact cacgttgtct ggatgggggt ttatcgccgc gatgttattg
     3421 ttaaaaataa cattaaattt attgccggat tacatcatca ggatattgtc tggacaacag
     3481 aattcatgtt taatgcgctg cgtgcgcg
//
LOCUS       DAZFWP020000281         3452 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFWP020000281 DAZFWP020000000
VERSION     DAZFWP020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.283"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..723
                     /locus_tag="ACHK0Z_RS24805"
                     /old_locus_tag="pgaptmp_004961"
     CDS             <1..723
                     /locus_tag="ACHK0Z_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU4504811.1"
                     /translation="ALPENFRIHDLEKFNLNRFRFRGALSTASIDDFTRYSKDLADEG
                     TRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKAAPFSALLSVNGERNSQKSLA
                     EWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQTADFEDNDFSGKRSLMESVE
                     AKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEF
                     RDLLVEKFKDSKVETFIGTFTA"
     gene            852..1388
                     /gene="yfdR"
                     /locus_tag="ACHK0Z_RS24810"
                     /old_locus_tag="pgaptmp_004962"
     CDS             852..1388
                     /gene="yfdR"
                     /locus_tag="ACHK0Z_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4504812.1"
                     /translation="MSFIKTFSGKHFYYDRINKDNIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            1379..1741
                     /locus_tag="ACHK0Z_RS24815"
                     /old_locus_tag="pgaptmp_004963"
     CDS             1379..1741
                     /locus_tag="ACHK0Z_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504813.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LESKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPETKK"
     gene            1738..1953
                     /locus_tag="ACHK0Z_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             1738..1953
                     /locus_tag="ACHK0Z_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504814.1"
                     /translation="MSVIKTHTGIVITRDGEKRMKLHSTETSWVAGRCESYDKKTGYR
                     WGAPNMRRRLLLDSIRPIKQVATREQN"
     gene            2013..2219
                     /locus_tag="ACHK0Z_RS24825"
                     /old_locus_tag="pgaptmp_004965"
     CDS             2013..2219
                     /locus_tag="ACHK0Z_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4504815.1"
                     /translation="MLNLDCVPISTYCKETGETPEAINKRVQRGVWREGVQVLKVEGV
                     KERWIDLSEVAKWARQNCSNYRAA"
     gene            2180..3355
                     /locus_tag="ACHK0Z_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     CDS             2180..3355
                     /locus_tag="ACHK0Z_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGU4504816.1"
                     /translation="MGQTKLLKLPRGVTIRKHRQGETINITFTYKGVRCREPLSNLEV
                     TPKNIKYAERTLGEIHNKIERGTFIYAEYFPRSARLKIFGNAAAGKTVKMYLDEYLEI
                     CETRKLSPSTIGGYKKCRSALASLHICPASELTPATLKAWIQSQKTTLKTIRNQLSFL
                     RSALDEAVTDGVLQINPVSLVTASRYQSDKSEAESSYVVDPLSPAEVDALLAAAGNKQ
                     WENLFRFAIHTGLRSSELCALRWRDIDFVGKTAHVQSASVVGVIKGTKTKAGTRKVEL
                     TEEAMLALINQKPFTFMKDATVFEDPKTNKPWASADAIRKKAWVPTLRKAGIRYRNPY
                     QTRHTYATKYISQGANLFWLAGQMGHKGPEMLFRHYGRYLSDYDNHSSTTLTSNCKK"
BASE COUNT         1026 a          760 c          821 g          845 t
ORIGIN      
        1 gcattacctg aaaatttccg cattcatgat ctggaaaaat ttaatttaaa tcgcttccgt
       61 ttccgtggtg cgctttccac tgccagcatc gatgacttta cccgttattc taaagatctt
      121 gcagatgaag gcacccgctg ctttatcgat gctgataata tgcgtgccgt cagtgtgctt
      181 aacctgggta ctattgatga accaggtcac gcagataaca ccgccactct caaactgaaa
      241 aaggcagcgc cgttctctgc tctgttgtct gttaatggcg agcgtaactc ccagaagtca
      301 ctggcagaat ggattgaaga ctgggccgac taccttgtgg gctttgatgc taatggtgac
      361 accattcagg caacaaaagc ggctgcggcg gtccgtaaaa tcacgattga agcaaaccag
      421 actgctgatt ttgaagataa tgacttcagc ggcaaacgct ccctgatgga gtctgtcgaa
      481 gcgaagacca aagacattat gccagtggca tttgagttta aatgcgttcc gtttgaaggt
      541 ctgaaagaac gtccgtttaa attacgcctc agcattatca ctggcgatcg tccggtactg
      601 gttctacgca ttattcagct ggaagcggtg caggaagata tggctaacga atttcgtgat
      661 ctgcttgttg agaaattcaa ggacagcaaa gtagaaacct ttattgggac tttcaccgcc
      721 tgatttcatt accgcaaatg cccctgcggg ggcatttatg gaaacgtaat tgactcaata
      781 atcgcctgaa ggcgagggtt ttctttaacc aaaattcagc gcggtgcagc gcatataacg
      841 tggagaacaa aatgtcattt attaaaactt tttccgggaa gcatttttat tatgacagga
      901 taaataaaga caacatcgat attaacgata tcgcggtttc cctttcaaat atctgtcgct
      961 ttgccggcca tctttcacac ttctacagcg tcgcccaaca tgcggtgctt tgcagccagc
     1021 tggtaccgca ggaatttgct tttgaagcgt taatgcatga tgcaacagaa gcgtattgcc
     1081 aggacatccc cgcaccactg aaacgccttc ttcctgacta taaacggatg gaagaaaaaa
     1141 tagacgccgt aatccgtgag aaatacgggt tacccccggt tatgagcacg cctgtgaaat
     1201 atgccgatct catcatgctg gcaaccgaac gccgcgatct cgggcttgat gatggctctt
     1261 tctggcctgt actggaaggc atcccggcaa cagagatgtt caacgtgatt ccactggcac
     1321 cgggccatgc ctacgggatg tttatggaac gttttaacga gttatcggag ttacgcaaat
     1381 gcgcatgaat gttttcgaaa tggaagggtt tcttcgtggg agatgtgtac cgcgagatct
     1441 gaaagtgaac gaaacaaatg ctgagtacct ggtacgtaaa ttcgacgcgc ttgaagctaa
     1501 atgtgcggca ctggaaagca aaataatacc agtgtcagct gaactgcctc cagcaaatga
     1561 aagtgttctg ttatttgatg ctaacggaga aggctggctg attggctggc gttctctctg
     1621 gtacacctgg ggacaaaaag aaaccggaga atggcagtgg acatttcagg tcggggacct
     1681 tgaaaacgtc aatatcactc actgggcagt aatgccgaaa gcaccagaga ctaagaaatg
     1741 agcgtgataa aaactcatac aggaattgtt atcacccgag acggtgaaaa gcggatgaaa
     1801 ttacattcca ctgaaacgtc ctgggttgcc ggacgttgtg aatcctacga caaaaagact
     1861 ggttaccgtt ggggtgcacc taacatgcgt cgccgtctgc tactggacag catcaggcca
     1921 ataaaacaag tagcaaccag agaacaaaat taattatcag gactggaatt tgatattact
     1981 gcccatgtgc agcgggctaa gtggagaaac atatgctgaa cctcgattgt gttcctatct
     2041 caacttattg caaagaaact ggcgaaactc ctgaagcaat aaacaaacgt gtacagcgcg
     2101 gtgtttggcg tgaaggtgtt caggttttaa aggttgaagg cgttaaggag aggtggattg
     2161 atcttagtga ggttgcaaaa tgggccagac aaaactgctc aaactaccgc gcggcgtaac
     2221 aatcaggaaa caccgccagg gcgaaacgat caatataact ttcacctaca aaggagttag
     2281 atgtcgtgag ccgctttcca atctggaagt aacaccaaag aacattaaat acgccgagcg
     2341 tacactcggc gaaatccata ataagatcga aaggggaaca ttcatttatg cagaatattt
     2401 tccccgttct gctcgtttga aaatttttgg taatgctgct gcaggcaaaa cggtaaagat
     2461 gtacctggac gaatatcttg aaatctgcga aacaagaaaa ctttcaccct ctacgattgg
     2521 tggctataaa aaatgccgta gtgcgttagc ctcacttcac atttgccctg caagtgaatt
     2581 aacaccagca accctgaaag cgtggattca aagccagaaa acaaccttaa aaaccattcg
     2641 caaccagtta tctttcctgc ggtcagcact tgatgaagcc gtgaccgatg gggtacttca
     2701 aattaacccc gtatcgttag taacagcttc gcgctaccaa agtgataagt cagaagcaga
     2761 aagcagctat gtggttgatc cgctatcacc agcagaagtt gatgcattac tagcagcagc
     2821 cggaaacaaa caatgggaaa atctgttccg gttcgctata catacaggcc tgcgtagttc
     2881 tgaactatgt gcccttcgat ggcgtgatat cgactttgtt ggaaaaacag cacatgtcca
     2941 gagcgcaagt gttgttggtg ttatcaaagg gaccaaaaca aaagccggta ctcgtaaagt
     3001 tgaactgaca gaagaggcaa tgttggcgct gataaatcag aagccattta cgttcatgaa
     3061 agatgctacg gtctttgaag atccaaagac caataagcct tgggcaagtg ctgatgcaat
     3121 caggaaaaaa gcatgggtgc caacattgcg aaaagcaggt attcgctaca gaaacccata
     3181 tcagaccagg cacacttatg ctactaagta cattagccaa ggagctaatc tcttctggct
     3241 agcagggcag atggggcata aggggcccga aatgttgttt cgtcattatg ggcgttactt
     3301 gagtgattat gacaaccaca gctcaacaac gctcacaagt aattgcaaaa agtagccttg
     3361 agcaaggtaa agcatgttat tcaaagaagc ctatttcgtt caaaacgaga ggtcctccac
     3421 aaatccacgc ttgaataggt attctttaca at
//
LOCUS       DAZFWP020000282         3408 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFWP020000282 DAZFWP020000000
VERSION     DAZFWP020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.284"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..416
                     /gene="yfdR"
                     /locus_tag="ACHK0Z_RS24835"
                     /old_locus_tag="pgaptmp_004967"
     CDS             <1..416
                     /gene="yfdR"
                     /locus_tag="ACHK0Z_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4504817.1"
                     /translation="SHFYSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKR
                     LLPDYKRMEEKIDAVIREKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEG
                     IPATEMFNVIPLAPGHAYGMFMERFNDLSELRKCA"
     gene            407..769
                     /locus_tag="ACHK0Z_RS24840"
                     /old_locus_tag="pgaptmp_004968"
     CDS             407..769
                     /locus_tag="ACHK0Z_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504818.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            769..1074
                     /gene="yfdT"
                     /locus_tag="ACHK0Z_RS24845"
                     /old_locus_tag="pgaptmp_004969"
     CDS             769..1074
                     /gene="yfdT"
                     /locus_tag="ACHK0Z_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HGU4504819.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            990..1169
                     /locus_tag="ACHK0Z_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /pseudo
     CDS             990..1169
                     /locus_tag="ACHK0Z_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1301..2464
                     /gene="intD"
                     /locus_tag="ACHK0Z_RS24855"
                     /old_locus_tag="pgaptmp_004971"
     CDS             1301..2464
                     /gene="intD"
                     /locus_tag="ACHK0Z_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGU4504820.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(2480..2556)
                     /locus_tag="ACHK0Z_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     tRNA            complement(2480..2556)
                     /locus_tag="ACHK0Z_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2520..2522),aa:Arg,seq:tct)
     gene            2799..>3408
                     /gene="fimZ"
                     /locus_tag="ACHK0Z_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             2799..>3408
                     /gene="fimZ"
                     /locus_tag="ACHK0Z_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU4504821.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDY"
BASE COUNT         1034 a          736 c          824 g          814 t
ORIGIN      
        1 tttcgcactt ctacagcgtc gcccaacatg cggttctttg cagccagctg gtgccgcagg
       61 aatttgcttt tgaagcgtta atgcatgatg caacagaagc gtattgccag gacattcccg
      121 caccactgaa acgccttctt cctgactata aacggatgga agaaaaaata gacgccgtaa
      181 tccgtgagaa atacgggtta cccccagtta tgagtacacc cgtgaaatat gccgatctca
      241 tcatgctggc aaccgaacgc cgcgatctcg ggcttgatga tggctctttc tggcctgtac
      301 tggaaggcat cccggcaaca gaaatgttca acgtgattcc actggcaccg ggtcatgcct
      361 acgggatgtt tatggaacgt tttaacgatt tatcggagtt acgcaaatgc gcatgaatgt
      421 tttcgaaatg gaagggtttc ttcgcgggaa atgtgtaccg cgagatctga aagtgaacga
      481 aacaaatgct gagtacctgt tacgtaaatt cgacgcgctt gaagctaaat gtgcggcact
      541 ggaaaacaaa ataataccag tgtcagctga actgccacca gcaaatgaaa gtgttctgtt
      601 atttgatgct aatggagaag gctggctgat tggctggcgt tctctctggt acacctgggg
      661 acaaaaagaa accggagaat ggcagtggac atttcaggtc ggggaccttg aaaacgtcaa
      721 tatcactcac tgggcagtaa tgccaaaagc accggaggct ggagcataat gaccacattt
      781 accaataaag aactgattaa agaaatcaaa gaacgaatca gcagcctaga ggttcgagac
      841 gatattgagc gccgtgctta tgaaatcgca ctcgtatctc tggaagtaga gccagatgaa
      901 cgcgaagcct atgaattatt catggaaaag cgtttcggtg acttagtaga tcgtcggaga
      961 gcaaaaaacg gcgataacga atacatggca tgggatatga ctctcggttg gatcgtctgg
     1021 cagcaacgag ctggtatcca tttttcaaca atgtcacagc aagaggtgaa ataatggagc
     1081 catacagcct cacactcgat gaggcctgtc attttctcaa aatatccaga ccgactgcca
     1141 ttaactggat acgcacaggc cgcctacagg caacacgtaa agatccaacc aagccaaaat
     1201 ctccttacct cacaacacgg caagcttgca ttgcggcgct tcagtctccg ctgcatactg
     1261 tccaggtgag cgcgggtgat ggcataacag aggaaagaaa atgtcactct tccgcagaaa
     1321 tgaaatatgg tatgcctcgt attcgctccc gggcgggaaa cgaattaagg aatctcttgg
     1381 cacaaaggac aagcggcaag ctcaggagtt gcacgacaag cgaaaagcag aactctggcg
     1441 agtagaaaag ctaggggatt tacctgatgt cacttttgaa gaggcctgcc taagatggct
     1501 tgaggaaaaa gctgataaaa aatctctcga ttcagataaa agccggattg agttctggct
     1561 tgaacatttt gagggtataa ggcttaaaga tatctcggag gcaaagattt actctgctgt
     1621 aagcagaatg cataacagaa agacgaaaga aatatggaaa cagaaagttc aggccgccat
     1681 caggaaaggt aaagaaccgc ctgtttatga accaaagcca gtatcaactc agacaaaggc
     1741 aaagcatctt gccatgataa aggccattct ccgtgctgca gaacgcgact ggaagtggct
     1801 ggaaaaagcg cctgtcatca agataccagc ggtcagaaac aagcgagtca gatggctgga
     1861 aaaggaggaa gcaaaacgcc ttattgatga gtgccccgaa ccactgaaat ctgtcgtcaa
     1921 gtttgcgctg gcaactggtc tgagaaagtc gaacatcata aatctggaat ggcaacaaat
     1981 cgacatgcag cgacgagttg cctgggtgaa tccagaagag agcaaatcaa accgcgccat
     2041 tggtgtggcg ctgaacgata ccgcctgtaa agtgttgcgt gatcaaatag gcaagcatca
     2101 caaatgggtg tttgtacata ccaaggcggc taagcgagca gatggaacat caacgcctgc
     2161 ggtcaggaag atgcgcatcg acagcaagac atcatggcta tcagcttgtc gtcgtgcagg
     2221 aattgaagat ttccgtttcc atgacctcag acacacctgg gcaagctggc tgatccagtc
     2281 aggcgtccca ttatctgtgc ttcaggaaat gggcggatgg gagtccatag aaatggttcg
     2341 taggtatgct caccttgcgc ctaatcattt gacagagcat gcaaggaaaa tagacgacat
     2401 ttttggtgat aatgtcccaa atatgtccca ctgtggaatt atggaggata taaagaaggc
     2461 gtaactgatt gaattgtaat ggcgcgccct gcaggattcg aacctgcggc ccacgactta
     2521 gaaggtcgtt gctctatcca actgagctaa gggcgcgttg ataccgcaat gcggtgtaat
     2581 cgcgtgaatt atacggtcaa cccttgctga gtcaatggct tttgatctgg ttgctgaaca
     2641 agtgaacgac cgcgtctgat tttctgattt atttcgctat agcggcaaac aaacacacac
     2701 cgctgcgcgt ctgaatcaag aaaacccgta ttttcatgta tcaaagtaca atttaccgcc
     2761 ctaacggaaa attgtccgct cctatgagac tggtaactat gaaaccaacg tcggtgatca
     2821 ttatggatac tcatcctatc atcagaatgt ctattgaagt tctgttgcaa aaaaacagtg
     2881 aattgcagat tgtcctgaaa acggatgatt accgcataac catcgattat ctccgaaccc
     2941 gtcctgttga tttaatcatt atggatatag acttgcccgg aacagacggt tttaccttcc
     3001 tgaaaaggat caaacaaatc cagagcacag tgaaagtgtt atttttatca tcgaaatcag
     3061 aatgctttta tgctggcaga gcgatacaag ctggtgctaa cggttttgtc agtaaatgca
     3121 atgatcagaa tgatattttt catgccgttc agatgatcct ctccggatac acgttttttc
     3181 ccagcgaaac gcttaactat ataaaaagca ataaatgtag tacgaatagt tcaacggtca
     3241 ctgtgctatc taatcgtgaa gtgaccatat tacgttatct ggttagcgga ttatctaata
     3301 aagaaattgc cgataagtta ttacttagca ataaaacagt tagtgcgcat aaatctaata
     3361 tttatggcaa gctaggtttg cattcaattg tagagcttat cgactacg
//
LOCUS       DAZFWP020000283         3336 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFWP020000283 DAZFWP020000000
VERSION     DAZFWP020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.285"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..193
                     /locus_tag="ACHK0Z_RS24870"
                     /old_locus_tag="pgaptmp_004974"
     CDS             <1..193
                     /locus_tag="ACHK0Z_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HGU4504822.1"
                     /translation="LTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQ
                     RDNALLMIGKVIDYKEDVI"
     gene            251..829
                     /gene="espA"
                     /locus_tag="ACHK0Z_RS24875"
                     /old_locus_tag="pgaptmp_004975"
     CDS             251..829
                     /gene="espA"
                     /locus_tag="ACHK0Z_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGU4504823.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNEVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIAEVQSSSNKDAKAK
                     LPQEVIDYIKDPRNGITVSDVKDITVELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARADIQSLQYRTISAISLGK"
     gene            842..1984
                     /locus_tag="ACHK0Z_RS24880"
                     /old_locus_tag="pgaptmp_004976"
     CDS             842..1984
                     /locus_tag="ACHK0Z_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HGU4504824.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSAAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            2005..2949
                     /gene="espB"
                     /locus_tag="ACHK0Z_RS24885"
                     /old_locus_tag="pgaptmp_004977"
     CDS             2005..2949
                     /gene="espB"
                     /locus_tag="ACHK0Z_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGU4504825.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQQTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            2956..>3336
                     /gene="cesD2"
                     /locus_tag="ACHK0Z_RS24890"
                     /old_locus_tag="pgaptmp_004978"
     CDS             2956..>3336
                     /gene="cesD2"
                     /locus_tag="ACHK0Z_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGU4504826.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGI"
BASE COUNT          970 a          666 c          802 g          898 t
ORIGIN      
        1 gttaacagaa gactggctcc tggaaagccc ttctcgggta tcgattgtcg aagataaaca
       61 tgtttattat ttccacctat tgaaagactt ttttgcatca ttacctgacg cttgttttat
      121 tgatagggaa cagagagata atgcattatt aatgattggt aaagtaattg attataagga
      181 ggatgttatt tgatattgat tttttaattg tttttggtct tgctaagaaa gattattaag
      241 aggtatatac atggatacat caactgcaac atcagttgcc agtgcgaacg cgagtacttc
      301 gacatcgaca gtctatgact taggcagcat gtcgaaagac gaagtagttc agctatttaa
      361 tgaagtcggt gtttttcagg ctgcgcttct catgtttgcc tatatgtatc aggcacaaag
      421 cgatctgtcg attgcaaagt ttgctgatat gaatgaggca tctaaggagt caaccactgc
      481 ccaaaaaatg gctaatcttg tggatgctaa aattgctgag gttcagagca gttctaacaa
      541 ggatgcgaaa gccaaacttc ctcaagaagt gatcgactat ataaaagatc ctcgtaatgg
      601 cattacggtc agtgatgtaa aagacattac tgttgaatta ggcgctggtg atttgcaaac
      661 ggtgaaggca gctatttcgg ccaaatcgaa taacttgacc acggtagtga ataatagcca
      721 gcttgaaata cagcaaatgt caaatacgtt aaacttatta acgagtgcac gtgctgatat
      781 tcagtcactg caatacagaa ctatttcagc aatatccctt ggtaaataac cggagataac
      841 tatgctcaat gtaaatagcg atatccagtc gatgaggtct ggggccagcg ctgctacggc
      901 tacttcaggt attaatcagc ctgaagtgac ttccgcgctg gatttacaac tggttaaatc
      961 cacggcccca tcagcaagtt ggacagaaag taccgcttta ccaacgccac cggcaggcca
     1021 ctcattagtg acgccctctg ctgctgagga tgtcctgagt aaactgtttg gtggtattag
     1081 tggtgaagtt aggagtcgaa cagagggaac cgaaccacag cgcagcactc agaatgcaag
     1141 ctctggctat ccttatcttt ctcaggtgaa taacgttgac cctcaggcca tgatgatgat
     1201 ggtcaccctg ttatccctgg atgcttcagc gcagaaagtt gcgagtatga aaaactccaa
     1261 cgagatctac acggatgggc aaaataaggc gctggataat aaaacgctgg agtttaaaaa
     1321 gcagctcgaa gaacaacaga aagccgaaga gaaagcacaa aaaagtaaaa ttgttggtca
     1381 ggtctttggt tggttgggcg tcgcggtaac tgctattgca gctattttta acccggcact
     1441 ctgggctgtt gttgccgtta gcgcaacagc aatggcactg caaactgcag ttgatgtaat
     1501 gggcgataag gccccgcagg cgttaaagac agcagcgcaa gtctttggcg gagtatctat
     1561 tgctgcaagt attctgacag ccggcattgg cggggtgtct tcacttatgt ctaaagttgg
     1621 cgatgtcgct aacaaagttg gttcaaacat cgtgaaaaca gttactaaac tggccgatgt
     1681 atttgttgag aacgttgttt caaaagttgc ggcaacggca aatggattta cgacctctgc
     1741 acgttctatt gggacaactg tgctgaataa tgattctgcg caatacagca tattggctca
     1801 gttatctgct tacgcggtgc aaaacttaac tcgacagagt gaaaacctgg gtgagagtgc
     1861 gaaggttgag ctggataaag ctgcagccga gttgcgaaac caggcaagct atttacaaaa
     1921 tgcttctcag ttaatatccg actcagcacg agtaaatagc cgtattgtta gtggtcgagt
     1981 ttaattatta aagagaattt aattatgaat actattgatt atactaatca agtaatgacg
     2041 gttaattctg tttcggagaa tactaccggc tctaatgcaa ttaccgcatc tgctattaat
     2101 tcatctttgc ttaccgatgg taaggtcgat gtttctaaac tgatgctgga aattcaaaaa
     2161 ctcctgggca agatggtgcg tatattgcag gattaccaac agcaacagtt gtcgcagagc
     2221 tatcagatcc aactggccgt ttttgagagc cagaataaag ccattgatga aaaaaaggcc
     2281 gctgcaacag ccgctctggt tggtggggct atttcatcag tattggggat cttaggctct
     2341 tttgcagcaa ttaacagtgc tacgaaaggc gcgagtgata ttgctcaaca aaccgcctct
     2401 acatcttcta aggctattga tgcggcttct gatactgcga ctaaaacgtt gactaaggca
     2461 acggaaagcg ttgctgatgc tgttgaagat gcatccagcg tgatgcagca agcgatgact
     2521 acagcaacga gagcggccag ccgtacatct gacgttgcag atgacattgc cgattctgct
     2581 cagagagctt ctcagctggc tgaaaacgct gcagatgccg ctcagaaggc aagtcgggca
     2641 agccgcttta tggctgcagt agataagatt actggctcta caccatttat tgccgttacc
     2701 agtcttgccg aaggcacgaa gacattgcca acaacggtat ctgaatcagt caaatctaac
     2761 catgagatta gcgaacagcg ttataagtct gtggagaact tccagcaggg taatttggat
     2821 ctgtataagc aagaagttcg cagagcgcag gatgatatcg ctagccgtct gcgtgatatg
     2881 acaacagccg ctcgcgatct cactgatctt cagaatcgta tgggtcaatc ggttcgctta
     2941 gctgggtaat tgatcatggt cgatacgttt aatgatgaag tgcttaatca ctatcttgaa
     3001 caaaaagggt acacaataca gaaggagttt ctttgtggca gtgccttttt tatcggatgg
     3061 cggattgaga cctctttttt ctcattagcg tacagacttg atgaacaaga actgattttg
     3121 tgctcttttg aagcacgtaa ccaaacaggg cttaacggcc ctgttttatc actgactcgc
     3181 ttgctcgaag agttgtacca ccatttttcg ggtattaaga aaatcagtgc gatgaaatct
     3241 aagattggtt cagattcaga acgtcaaaag cgcgaagagt tgtttaatta cttcatcaga
     3301 aagggcgctg tgcagcaaga aacagaagac ggaatt
//
LOCUS       DAZFWP020000284         3331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFWP020000284 DAZFWP020000000
VERSION     DAZFWP020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.286"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..1192
                     /gene="yiaY"
                     /locus_tag="ACHK0Z_RS24895"
                     /old_locus_tag="pgaptmp_004979"
     CDS             41..1192
                     /gene="yiaY"
                     /locus_tag="ACHK0Z_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU4504827.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1357..2895
                     /gene="aldB"
                     /locus_tag="ACHK0Z_RS24900"
                     /old_locus_tag="pgaptmp_004980"
     CDS             1357..2895
                     /gene="aldB"
                     /locus_tag="ACHK0Z_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU4504828.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3114..>3331)
                     /locus_tag="ACHK0Z_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             complement(3114..>3331)
                     /locus_tag="ACHK0Z_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504829.1"
                     /translation="EVCYLAFQQVETYHIEIMELLAKLMNNSSKTSTVQIKRIKPSII
                     YRLLLIGLGSPMVIYGLIRPLTIETRD"
BASE COUNT          811 a          825 c          901 g          794 t
ORIGIN      
        1 gtaagctgcc cacccttttt tacactttca ggagtgtgtt atggcatctt caactttctt
       61 tattccttct gtgaatgtca tcggcgctga ttcattgact gatgcaatga atatgatggc
      121 agattatgga tttacccgta ccttaattgt cactgacagt atgttaacga aattaggtat
      181 ggcgggcgat gtgcaaaaag cactggaaga acgcaatatt tttagcgtta tttatgatgg
      241 cacccaacct aaccccacca cggaaaacgt cgccgcaggt ttgaaattac ttaaagagaa
      301 taattgcgat agcgtgatct ccttaggcgg tggttctcca cacgactgcg caaaaggtat
      361 tgcgctggtg gcagccaatg gcggcgatat tcgcgattac gaaggcgttg accgctctgc
      421 aaaaccgcag ctgccgatga tcgccatcaa taccacggcg ggtacggcct ctgaaatgac
      481 ccgtttctgc atcatcactg acgaagcgcg tcatatcaaa atggcgattg ttgataaaca
      541 tgtcactccg ctgctttctg tcaatgactc ctctctgatg attggtatgc cgaagtcact
      601 gaccgccgca acgggtatgg atgccttaac gcacgctatc gaagcgtatg tttctattgc
      661 cgccacgccg atcactgacg cttgtgcact gaaagccgtg accatgattg ccgaaaacct
      721 gccgttagcc gttgaagatg gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca
      781 gttcctcgcc ggtatggcgt tcaataatgc ttctctgggt tatgttcatg cgatggcgca
      841 ccagctgggc ggtttctaca acctgccaca cggtgtatgt aacgccgttt tgctgccgca
      901 tgttcaggta ttcaacagca aagtcgccgc cgcacgtctg cgtgactgtg ccgctgcaat
      961 gggcgtgaac gtgacaggta aaaacgatgc ggaaggtgct gaagcctgca ttaacgccat
     1021 ccgtgaactg gcgaagaaag tggatatccc ggcaggccta cgcgacctga acgtgaaaga
     1081 agaagatttc gcggttctgg cgactaatgc cctgaaagat gcctgtggtt ttactaaccc
     1141 gatccaggca actcacgaag aaattgtggc gatttatcgc gcagcgatgt aatcatcatt
     1201 tccacaacgg ctggcaaatt gtcagccgct ttttcaacta tctctgtaac ccttgcccgt
     1261 aaattcgtga tagctgtcgt aaagctgtta ccgactggcg aagatttcgc cagtcacgtc
     1321 tacccttgtt atacctcaca ccgcaaggag acgatcatga ccaataatcc cccttcagca
     1381 cagattaagc ccggcgagta tggtttcccc ctcaagttaa aagcccgcta tgacaacttt
     1441 attggcggcg aatgggtagc ccctgccgac ggcgagtatt accagaatct gacgccggtg
     1501 accgggcagc tgctgtgcga agtggcgtct tcgggcaaac gagacatcga tctggcgctg
     1561 gatgctgcgc acaaagtgaa agataaatgg gcgcacacct cggtgcagga tcgcgcggcg
     1621 attctgttta agattgccga tcgaatggaa caaaacctcg agctgttagc gacagctgaa
     1681 acctgggata acggcaaacc cattcgcgaa accagtgctg cggatgtacc gctggcgatt
     1741 gaccatttcc gctatttcgc ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt
     1801 gatagcgaaa ccgtggccta tcatttccat gaaccgttag gcgtggtggg gcagattatc
     1861 ccgtggaact tcccgctgct gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc
     1921 aactgtgtgg tgctgaaacc cgcacgtctt accccgcttt ctgtactgct gctaatggaa
     1981 atcgtcggtg atttactgcc gccgggcgtg gtgaacgtgg tcaacggcgc aggtggggaa
     2041 attggcgaat atctggcgac ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg
     2101 gaagtgggcc aacaaattat gcaatacgcc acgcaaaaca ttattccggt gacgctggag
     2161 ctgggcggca aatcgccaaa tatcttcttt gctgatgtga tggatgaaga agatgccttt
     2221 ttcgataaag cgctggaagg ctttgcactg tttgccttta accagggtga agtttgcacc
     2281 tgtccgagtc gtgccttagt gcaggagtca atctacgaac gctttatgga acgcgccatc
     2341 cgccgtgtcg aaagcattcg cagcggtaac ccactcgaca gcgtgacgca aatgggcgcg
     2401 caggtttctc acgggcaact ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag
     2461 ggcgcagacg tgctcaccgg cgggcggcgc aagctgctgg aaggtgaact gaaagacggc
     2521 tactacctcg aaccgacgat tctgtttggt cagaacaata tgcgggtgtt ccaggaggag
     2581 atttttggcc cggtgctggc ggtgaccacc ttcaaaacga tggaagaagc gctggagctg
     2641 gcgaacgata cgcaatatgg cctgggcgca ggcgtctgga gtcgtaacgg taatctggcc
     2701 tataagatgg ggcgcggcat tcaggccggg cgcgtgtgga ccaactgcta tcacgcttac
     2761 ccggcacatg ctgcgtttgg tggctacaaa caatcaggta tcggtcgcga aacccacaag
     2821 atgatgctgg agcattacca gcaaaccaag tgcctgctgg tgagctactc ggataaaccg
     2881 ttggggctgt tctgatataa gaagctggtc gcattgggta ttcattgcct gatgcgacgc
     2941 ttacgcgtct tatcatgcct acgggaacct gaccgtaggc cggataaggc gtacacgccg
     3001 catccggcat aaacaaagcg cacgttgtta caatttacgt tttaaaccta cgtgcgcaat
     3061 aacccagact acgccaacag aagtgcgttt ctggggcgtg ttttgtcagg agtttaatct
     3121 cgcgtttcga tggtgagcgg gcgaatcagg ccgtaaatca ccattggtga tccgaggcca
     3181 atcagcaata aacggtagat aattgaaggt ttaatacgct taatctgtac tgtggatgtt
     3241 ttactggagt tattcattag tttggccaat agttccatta tttcaatatg ataagtctcg
     3301 acctgttgaa aagccaggta gcacacttca g
//
LOCUS       DAZFWP020000285         3289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFWP020000285 DAZFWP020000000
VERSION     DAZFWP020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.287"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..366
                     /locus_tag="ACHK0Z_RS24910"
                     /old_locus_tag="pgaptmp_004982"
     CDS             <1..366
                     /locus_tag="ACHK0Z_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGU4504830.1"
                     /translation="GPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAY
                     TAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAE
                     AVKALGFLKQKATEQKVAA"
     gene            363..1043
                     /locus_tag="ACHK0Z_RS24915"
                     /old_locus_tag="pgaptmp_004983"
     CDS             363..1043
                     /locus_tag="ACHK0Z_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4504831.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1040..1222
                     /locus_tag="ACHK0Z_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             1040..1222
                     /locus_tag="ACHK0Z_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU4504832.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            1195..1386
                     /locus_tag="ACHK0Z_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             1195..1386
                     /locus_tag="ACHK0Z_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4504833.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            1463..1678
                     /locus_tag="ACHK0Z_RS24930"
                     /old_locus_tag="pgaptmp_004986"
     CDS             1463..1678
                     /locus_tag="ACHK0Z_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4504834.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1777..1998
                     /locus_tag="ACHK0Z_RS24935"
                     /old_locus_tag="pgaptmp_004987"
     CDS             1777..1998
                     /locus_tag="ACHK0Z_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4504835.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(2209..2811)
                     /locus_tag="ACHK0Z_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             complement(2209..2811)
                     /locus_tag="ACHK0Z_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504836.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWTLNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            3054..3221
                     /locus_tag="ACHK0Z_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             3054..3221
                     /locus_tag="ACHK0Z_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504837.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
BASE COUNT          997 a          743 c          768 g          781 t
ORIGIN      
        1 ggaccgtggc agtcgcatcc caaacggatg ttacggcata aagccatgat tcagtgtgcc
       61 cgtctggcct tcggatttgc tggtatctat gacaaggatg aagccgagcg cattgtcgaa
      121 aataccgcat acactgcaga acgtcagccg gaacgcgaca tcactccggt taacgatgaa
      181 accatgcagg agattaacac tctgctgatt gccctggata aaacatggga tgacgactta
      241 ttgccgctct gttcccagat atttcgccgc gacattcgag catcgtcaga actgacacag
      301 gccgaagcag tgaaagctct tggattcctg aaacagaaag ccactgagca gaaggtggca
      361 gcatgacacc ggacattatc ctgcagcgta ccgggatcga cgtgagagct gtcgaacagg
      421 gggatgatgc atggcacaaa ttacggctcg gcgtcatcac cgcttcagag gttcacaatg
      481 tgatagcaaa accccgctcc ggaaagaaat ggcctgacat gaaaatgtcc tacttccaca
      541 ccctgcttgc cgaggtttgc accggtgtgg ctccggaagt taacgctaag gcgctggcct
      601 ggggaaaaca gtacgagaac gacgccagag ccctctttga gttcacttcc ggcgtgaatg
      661 ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg caccgcctgc tctcccgatg
      721 gtttatgcag tgacggcaac ggccttgaac tgaaatgccc gtttacctcc cgggatttca
      781 tgaagttccg gctcggtggt ttcgaggcca taaagtcggc ttacatggcc caggtgcagt
      841 acagcatgtg ggtgacgcga aaagatgcct ggtactttgc caactatgac ccgcgcatga
      901 agcgtgaagg cctgcattat gtcgtgattg agcgggatga aaattacatg gcgagttttg
      961 acgagatggt gccggagttc atcgaaaaaa tggacgaggc actggctgaa attggttttg
     1021 tatttgggga gcaatggcga tgaagcatcc tcacgataat atccgggtag gcgcgatcac
     1081 tttcgtctac tccgttacaa agcgaggctg ggtatttccc ggtctttctg ttatccgaaa
     1141 tccactgaaa gcacagcggc tggctgaaga gataaataat aaacgagggg ctgtatgcac
     1201 aaagcatctc ctgttgaatt aagaacgagt atcgagatgg cacatagcct tgctcaaatt
     1261 ggagtcaggt ttgtgccaat accagtagaa acagacgaag aatttcatac gttagccgca
     1321 tccctttcac aaaagctgga aatgatggcg gcgaaagcag aagcaaacga gagagacccg
     1381 gcatgacaac aacagaatgc atttttctgg cagcgggctt catattctgt gtgcttatgc
     1441 ttgccgacat gggacttgtt caatgacacc tcagcaagaa aacgcccttc gcagcattgc
     1501 ccgtcaggct aattctgaaa tcaaaaaagc cagacagcag tttccggata aaaacgtcga
     1561 tgacatttgc cgtagcgtac tgaagaagca ccgcgaaacg gtaacgctga tgggattcac
     1621 accgactcat ttaagcctgg cgatcggcat gttaaacggc gtctttaagg aacggtgagc
     1681 atgaaaagca aaatcatcag ggagctacag gctccttttt tattgttcgc attcaccctc
     1741 aagcgtatta accaacaatt cagggattaa tggaagatgg cagacatcat tgattcagca
     1801 tcagaaattg aagaattaca gcgcaacaca gcaataaaaa tgcgccgcct gaaccaccag
     1861 gctatatctg ccactcattg ttgtgagtgt ggcgatccca tagatgagcg aagacgcctg
     1921 gccgttcagg gttgtcggac ttgtgcaagt tgccaggagg atctggaact tatcagtaaa
     1981 cagagaggtt cgaagtgagc gaaattaact agaagccaaa gataaaatca tcgctgagca
     2041 ggagaaaatc gctaacggag aaaagacagt aagtcagtat atgaaaaccg catgatatca
     2101 tcagataaaa atcgatcgta aagcgaaata ttaataccag aataaacgag tcgaggtaaa
     2161 ttatattacc tcgataaatt aactaaaact tgcccgctat atactatctc attcagtatc
     2221 atcacgcgcg gtctgtgcat atgttactac cgcacctaat gtattaattt tcttttcaac
     2281 atagataata ttatcgtact cataattgcc atacggatag caaatgcgaa tattctcatg
     2341 tagatcgggg tcatccacct cagctccaga acaacttttt gaactaccgg aagtataccg
     2401 atacggtgca acataagacg atgtctctcc aggcaaaaaa taagttagtg ttgtaagggg
     2461 tataatcaga aaaaatccag caaatatgca catccctgca taaaccttaa ggtatgctga
     2521 cagactcttc cagccgcttt gttttactat ccccttctta acccaaaaca gagataacag
     2581 aaaagctatt cccatgctaa acagaatgta atagtgggat atactctgat taagaaacgt
     2641 gaccctgtaa atatctgccc gccaccagaa gaaaaggaaa ataaagatca ggcctgaaac
     2701 tgtcatgcaa atcaaataag gatacgaatc ttttttcatg tttagcgtcc ataaaatttt
     2761 tcctgccccg gacaaattta ccatccattt tttgcgcaga aaatagctca ttacttactg
     2821 cacaataata cacaaaattg cgtaaatttt ttgcatggat tttagctctt tcagccgacg
     2881 tttaaggggt aaatagcatt tcctaaaagc aactgcacca acccaacaga atgagctacc
     2941 gcttacgttg agagcaaaaa agtgtatagc agcaatgaac agcatcctcg cactgacgag
     3001 gatttctttt atctgaactc gctacggcgg gttttgtttt atggagatga taaatgcact
     3061 tccgagtcac aggtgaatgg aatggagaac cattcaacag agttatcgaa gcagagaaca
     3121 tcaatgactg ctatgaccac tggatgatat gggcgcagat agcacatgca gacataacca
     3181 atattcgaat tgaagaactg aaagaacacc aagccgcctg atggcggctt tttcttgcgt
     3241 gtaattgcgg agactttgcg atgtacttga cacttcagga gtggaacgc
//
LOCUS       DAZFWP020000286         3217 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFWP020000286 DAZFWP020000000
VERSION     DAZFWP020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.288"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHK0Z_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHK0Z_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU4504838.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHK0Z_RS24955"
                     /old_locus_tag="pgaptmp_004991"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHK0Z_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU4504839.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="ACHK0Z_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             1253..1522
                     /locus_tag="ACHK0Z_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU4504840.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="ACHK0Z_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="ACHK0Z_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU4504841.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEDYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..>3217
                     /gene="dsbA"
                     /locus_tag="ACHK0Z_RS24970"
                     /old_locus_tag="pgaptmp_004994"
     CDS             2602..>3217
                     /gene="dsbA"
                     /locus_tag="ACHK0Z_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU4504842.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLS"
BASE COUNT          804 a          775 c          793 g          845 t
ORIGIN      
        1 aggcccagtc ggaagactgg gcctttttgc gctggtgcag aaaaaggtcg gatgcgacac
       61 tggctgcgtc tgatccagcc tactcttgct tatccgttct gcttttgcat ccactccacc
      121 acaaaatcag ccagcccctc aacatcatta atatccagta atgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc aggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaactgtag atccagctct
      361 tcttcgtctg gtgtttccgt catcaaggcc catcgttgct ggctggcaac gatggtttgt
      421 gccgcgccag ccttgcgcag ctcatagcta tctttgcctg gcttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcagt
      541 tgtttcaaaa gcgtagtttt tccggtacca ctccacgcgg caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgttaata aatgcatctt
      661 tattatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc attacccggc
      721 gttctcctgc ttgcagatat tccagtaata aaggctcaat tgcgcggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      841 gattaagccg ggctgctaaa tcctcgggaa tataaggagt atcgcacgga caaaacaaaa
      901 accactcgcc tgcttcctgc tgcattactg aaagcattcc tgccagaggg cctgggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttgcctg atagatttcc tgatggcgat
     1021 tggcattaac cacgacgtga gagagctgcg tcataagcgc gtcagcgaca tgttgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcctgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1321 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca tctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagacttc aaaacggcgc tgttacgcgc
     1501 tcgcggcgta attaaagagt aaaagcttgt aagcggcgcc accaaaatca tcgtgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg atgctctatt tgagcaaggg atccgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 atcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1801 tcgaagaaca tcaatttgcg ttgcagctgg taaatgatga agttccggtc gcagcacctg
     1861 tggcctttaa cggtcagact ttattgaatc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2041 gtctgaatga atatctcatt gagcctcgca agctgtttga ggacgctaca ctgatacctt
     2101 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gatcgccgcc gttacagcac
     2161 actggcggga agacttcacc gttctgcggc tacatggaga ctgccacgcc gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagctattc
     2281 aggatttgtg gatgttgctc aatggcgata aagccgagca gtggatgcaa ctggaaacta
     2341 ttattgaaga ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttt ccgtggttaa ccggggaaga ttactggctg cgacagacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttgcaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccggtagctg gcgcgccgca agtgctggag tttttctctt tcttctgccc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aatgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg ggtggtgacc tgggcaaaga tctgactcag
     2881 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tgactgttcc gctgtttgaa
     2941 ggcgtacaga aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt gaaatctctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggtgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggcatggata ccagcaatat ggatgttttt
     3181 gttcagcagt atgctgatac agtgaaatat ctgtccg
//
LOCUS       DAZFWP020000287         3183 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFWP020000287 DAZFWP020000000
VERSION     DAZFWP020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.289"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(55..816)
                     /gene="kduD"
                     /locus_tag="ACHK0Z_RS24975"
                     /old_locus_tag="pgaptmp_004995"
     CDS             complement(55..816)
                     /gene="kduD"
                     /locus_tag="ACHK0Z_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU4504843.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(846..1682)
                     /gene="kduI"
                     /locus_tag="ACHK0Z_RS24980"
                     /old_locus_tag="pgaptmp_004996"
     CDS             complement(846..1682)
                     /gene="kduI"
                     /locus_tag="ACHK0Z_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU4504844.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1969..3150)
                     /gene="yqeF"
                     /locus_tag="ACHK0Z_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             complement(1969..3150)
                     /gene="yqeF"
                     /locus_tag="ACHK0Z_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4504845.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          839 a          879 c          718 g          747 t
ORIGIN      
        1 acaggtataa aaaaccctgc catgcggcag ggtcataaaa gtaagaagaa tgaattaacg
       61 cgccagccaa ccgccatcca cggcaatggt ataaccattc acataatctg aagcgctgga
      121 ggcaaggaac actatcggcc ccatcaggtc actcggcagt ccccaacgac cagctggaat
      181 gcggtcgaga atttccgcgc tacgttgttc atctgcccgt agttgttgag tattgttggt
      241 cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc attcgttcgc
      301 catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat
      361 cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc
      421 gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat
      481 gacatcgtcc cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc
      541 gttattcacc aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg
      601 aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt
      661 gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc
      721 ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc
      781 aactttacct tcgagagaaa atgcacttaa aatcataaca ataccttatt cgtttatgcc
      841 cacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac ctggttttca
      901 ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg aatcgaccag
      961 ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc
     1021 cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc
     1081 cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc
     1141 agccccatac tcaattggca ggtttccagc acatccggga caaaatattt gttaattgtg
     1201 cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact
     1261 tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt tgccggagtg
     1321 gcggtatcaa tactggcaaa gacaacttct tttgcacctt tacccacata cagggcgtcg
     1381 cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc gccaatattg
     1441 ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc
     1501 ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg
     1561 tggctgtaaa ccatggtgta ctcatcggcg acaaatacct cttcaaccaa aaattcattg
     1621 cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg tcttacgtcc
     1681 acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt
     1741 cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac
     1801 aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat
     1861 gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc
     1921 aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg
     1981 ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg
     2041 ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc
     2101 tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc
     2161 aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc
     2221 gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc
     2281 aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac
     2341 gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc
     2401 gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa
     2461 acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa
     2521 cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc
     2581 aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct
     2641 gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc
     2701 atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgtgcacc
     2761 ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc caccggcgat
     2821 gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagccc
     2881 agaaccgcaa acgtcattaa tagtgattgc agaaacgcta ttaggaagac cacctttaat
     2941 agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac
     3001 ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact
     3061 accaagttcc acggcggaat gacccgctaa cgcaccgcga aagcagccga taggtgtccg
     3121 taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata
     3181 gta
//
LOCUS       DAZFWP020000288         3162 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFWP020000288 DAZFWP020000000
VERSION     DAZFWP020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.290"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            34..675
                     /locus_tag="ACHK0Z_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             34..675
                     /locus_tag="ACHK0Z_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504846.1"
                     /translation="MNHEEMNQRISCLENEITELNKKLSVLMVSEDEKKRRDEQEAAF
                     YDDCIKIARRTFAKILQEKFLPTALSEKYSITVKSAGEEGNKRYFIASAPDKDQEWGD
                     NRPSFIVTSDDWNITIREDGKVTPASHQHSEALIEFAIDYLKNNKKQGLMKRIGRCMG
                     YLQVAAEIEALASGADKDAVVREALLRDFDNPPFKKVPAYWFHPGLTYLKGRI"
     gene            complement(685..999)
                     /locus_tag="ACHK0Z_RS24995"
                     /old_locus_tag="pgaptmp_004999"
     CDS             complement(685..999)
                     /locus_tag="ACHK0Z_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504847.1"
                     /translation="MYNDEHKYTACMQAMNEQFKSAFLKLIQQNHEAVKSIQAEPYGH
                     LTPPTLDIMSRILTPAMLLRLKDNINDWLNEELNYLECEWDHHYAKSQKERIFRRLSG
                     NR"
     gene            1128..1661
                     /locus_tag="ACHK0Z_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     CDS             1128..1661
                     /locus_tag="ACHK0Z_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4504848.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            1817..1999
                     /locus_tag="ACHK0Z_RS25005"
                     /old_locus_tag="pgaptmp_005001"
     CDS             1817..1999
                     /locus_tag="ACHK0Z_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504849.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            2012..2132
                     /locus_tag="ACHK0Z_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /pseudo
     CDS             2012..2132
                     /locus_tag="ACHK0Z_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2360..2545
                     /locus_tag="ACHK0Z_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             2360..2545
                     /locus_tag="ACHK0Z_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4504850.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            2631..2855
                     /locus_tag="ACHK0Z_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             2631..2855
                     /locus_tag="ACHK0Z_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504851.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQRGRVLIEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMASAALQPVC"
BASE COUNT          824 a          672 c          880 g          786 t
ORIGIN      
        1 gaccggcgta aggcggcgcg gggagagtag acgatgaacc atgaagaaat gaatcagcgc
       61 ataagttgcc tggaaaatga aatcactgaa ctgaataaaa aactgtcggt gctgatggtt
      121 tctgaagatg aaaaaaaacg ccgcgatgag caggaagcag cgttttacga tgattgcatc
      181 aaaattgctc gcaggacctt tgcgaagatt ttgcaggaaa agtttttacc gaccgcattg
      241 tcagaaaagt actccattac agttaaaagt gccggagagg aaggcaataa acgttatttt
      301 attgcgtctg caccggataa agaccaggaa tggggggata atcggccatc ttttattgtg
      361 acaagcgatg actggaatat cacgatccgt gaagatggaa aagtaacacc agcatcgcac
      421 cagcacagtg aggcgctcat tgaatttgcc attgattacc tgaagaacaa taaaaagcag
      481 ggactaatga agcgcattgg tcgttgcatg ggatatctgc aggtagctgc tgagattgaa
      541 gcgctggcca gtggtgcgga caaggatgca gttgtgcggg aggctcttct tcgtgatttt
      601 gataatccgc cctttaaaaa agtgccggct tactggtttc atccaggact gacttatctt
      661 aaaggacgta tataagctgg ctcgttatct gttgccggat aatcgacgga agatgcgttc
      721 tttttgtgat ttggcgtaat gatgatccca ctcacattca aggtagttta attcttcgtt
      781 taaccagtca tttatattgt ctttcagacg tagaagcatg gctggtgtta atattctgga
      841 cataatgtcg agagttggtg gtgtgaggtg tccatacggc tcagcctgaa tggattttac
      901 tgcttcgtgg ttttgctgaa tgagtttaag gaatgctgat ttaaattgtt cattcatggc
      961 ctgcatgcag gcggtatatt tatgttcatc gttatacatt gctaaatcct cgacgggaat
     1021 tgtcagatat atttcagcca tcaggtaaaa caccagtgcc caccactggc gggctgaaga
     1081 cttaacatat ccagggattc ggaaccgata aatcctgata aatatccatg aacaccaaaa
     1141 tcaaatacgg tctgtcggct gccgttctgg cgctgattgc cgcaggtgcg cctgcgcctg
     1201 aaatcctcga ccagtttctg gatgaaaagg aaggtaacca caccacggca taccgtgatg
     1261 gtgcggggat ctggaccatc tgccgtggag ccacccgggt ggatggtaag cctgttattc
     1321 ctggcatgaa gctgtcaaag gaaaaatgcg accgggttaa cgccattgaa cgtgataagg
     1381 cgctggcatg ggtggcgaaa aacatcaaag tgccactgac tgaaccccag aaagcgggga
     1441 tcgcgtcatt ctgcccgtat aacattggcc ccggtaagtg tttcccgtcg acgttttata
     1501 aacgaattaa tgcaggcgat cgcaggggag cgtgtgaagc gattcgctgg tggattaagg
     1561 atggtggcag agactgccgt atccgctcaa acaactgtta cggtcagata tcccgtcgtg
     1621 accaggagag cgcgctggcg tgctggggaa tcgacagata agcagaatat tttgctgaaa
     1681 aatgacgttg accaacgcgg acggataaca cgaaatcctg cgaactggca aaacctaagt
     1741 gaataaaagt aaaaccccgt ttgttggcag caagcggggt tttgtgtttc tgaccttgga
     1801 taaggcaagg gagaacatgg aaaagtataa acgaattctg ttgaggttga ctatgaaaaa
     1861 cggccttgaa ctgaaagcgc ctgtaactga tgacatcagc agagcgctgg cttttgctat
     1921 taagtgggtg gcggtcggta ttgctgtgtc tccgatgctg tatgggctgg caaaactggt
     1981 cattgcgttg aaatcgtgaa gaggattaag catgtcagac aagctcataa cgctggcgaa
     2041 gatcctctgt gtaatcgtcg gcatttcatt ttcactaatg ctggttgctc ttaggctgga
     2101 tgatgttgtc ttcgtcgggg atgctggggt gagggggata tgaaccgtgt tctgtgtgtg
     2161 gtgattattg tcctgctggt agcctgtggt gcgcttggtc tggggctgaa tcattaccgc
     2221 gataacgcca tcacctacaa agcgcagcgc gataaaaaag tcagagagct ggagctggcg
     2281 aacgcgacaa ttactgatat gcagatacgc cagcgtgatg tcgctgcgct cgatgcaaaa
     2341 tactcgaagg aattagccga tgcgagagct gaaaatgaaa ctctgcgtgc tgatgttgcc
     2401 gctggtcgta agcgcctgcg gatcaacgcc acctgccccg gtaccgtgcg tgaagccacc
     2461 ggcacctccg gcgtggataa tgcaaccggc ccccgactgg cagacaccgc tgaacgggat
     2521 tatttcatcc tcagagaacg gttgatgatg atgcagaagc agctggaagg ggcgcaggaa
     2581 tatatccgca ctcagtgtat taactagtat ttttgttatc cggagaatgc atgaagaaat
     2641 tactggtaac cgtaaagcct tttcagggaa caattccgtt ccgtgttttg cagcgtggtc
     2701 gtgttcttat tgaaggttcg ttcagtggta aatgtacgca attacactcc cggacctttc
     2761 aggtgaatgc cacgaatgaa gagctaaccg tggagtgtac gatgaatgcc gctaaatgcc
     2821 gcatggcatc cgctgcatta cagccagtgt gttgagcgac cttattatcc atgcgcggta
     2881 ttgtcgccgt attcccgtat taacagagac cgcagcccga cagggcgact cctctgcgcg
     2941 agtgtgcggg gataatcaaa aacgatacac accggggttt accgcgttaa cggagcgcgg
     3001 cgttgtcccc tcatggtcgc tggtccggtg cgatggtgga agaaaccgga ctacattaca
     3061 aatgataacc attatcattt ttcgggtcct cctggtgtgg tgggcctgaa cacggggcgg
     3121 gcggcgcgga aaaaggcgca ttttttgatt tttatggcac ca
//
LOCUS       DAZFWP020000289         3152 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFWP020000289 DAZFWP020000000
VERSION     DAZFWP020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.291"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..880
                     /locus_tag="ACHK0Z_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             <1..880
                     /locus_tag="ACHK0Z_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4504852.1"
                     /translation="KDTFTKNIGACRAFHSSISTGAGNWTTAQLIEWLDSQGAFNHPY
                     WMCKCSWSYGNNKIITDTGCGTIHLAGCVIEVMGNKGAMTIRVTTPSTSTGGGTTNAQ
                     FTYINHGGDYAPGWRRDYSTKNQQPAFALGQTGSTVGNDKAVGWNSISGVYNATIGGA
                     STLILHFNMNAGSCPAVQFRVNYKNGGIFYRSARDGYGFEADWSEFYTTTRKPSAGDV
                     GAYTKAECNSRFITGIRLGTKSSVQTWNGPGWNDKSGYVVTASINSNKDELIDTTQAR
                     PVQYCINGTWYNAGSI"
     gene            883..1416
                     /locus_tag="ACHK0Z_RS25030"
                     /old_locus_tag="pgaptmp_005006"
     CDS             883..1416
                     /locus_tag="ACHK0Z_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4504853.1"
                     /translation="MQHLKNIRSGNPKTVEQYELTRQFGVVWLYSEDDKNWYEEQKNF
                     QDDTIKIAYTSDGIIVAIDKDVSTINPEGLSVVEVADITANRRADISGKWMFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLIEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1444..1971)
                     /locus_tag="ACHK0Z_RS25035"
                     /old_locus_tag="pgaptmp_005007"
     CDS             complement(1444..1971)
                     /locus_tag="ACHK0Z_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4504854.1"
                     /translation="MIHLENIKIGNAKTPEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTAEEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1973..>3152)
                     /locus_tag="ACHK0Z_RS25040"
                     /old_locus_tag="pgaptmp_005008"
     CDS             complement(1973..>3152)
                     /locus_tag="ACHK0Z_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4504855.1"
                     /translation="KDRFLSNINVYSKGEVDKKKGMRQYVFNAPSNAVGGKWYPVIFQ
                     RSTGSTGELASRVIITTTSAGGNYEMNNCEFNGMVMPGGWTDRGSYAAGYFSTYQTNE
                     RAIHSIVTSLKEDDVCSVFYVEGRAFPVRVSAEEGLTVIVPTQDYTVGQTTYKWGATN
                     PATESTNAQAILDFNNGRGFYCSHSIFGINAIFSGNLGIGTANALGGNSIVLGDNDTG
                     FKQNGDGVLDAYANGVHVFRFINGSARSLKGIQAGESKFFTLSSANTAARNASFNLWG
                     NSSRPTVAELGDDSGWHFYSQRNTDNSVIFAVNGQIQPSNWGNIDSRYVKDVRLGSQQ
                     YYGVNNWQTWNFQCPSGHVLSGINVQDTGSNSADNIAGVYYRPVQKYINGTWYNVASV
                     "
BASE COUNT          900 a          705 c          663 g          884 t
ORIGIN      
        1 aaaagatacc ttcactaaaa atattggtgc atgtcgcgct tttcacagtt ctattagtac
       61 aggtgcaggg aactggacaa cggcacaatt gattgaatgg ctggattctc aaggggcatt
      121 caatcaccca tactggatgt gcaaatgttc atggtcatac ggcaataata aaattataac
      181 cgatactggc tgtggaacta ttcatcttgc aggttgcgtt attgaggtta tgggtaataa
      241 aggtgccatg accatccgtg taacaacgcc aagcacttcc accggtggcg gaaccactaa
      301 tgcgcaattt acttatataa accacggtgg agattacgca ccaggctggc ggcgtgacta
      361 cagtaccaaa aatcagcagc ctgcatttgc tttagggcag acaggaagca ctgttggaaa
      421 tgacaaagct gttggatgga actcaattag tggcgtttat aatgcgacta ttggtggagc
      481 atcaacattg attcttcact ttaatatgaa tgcggggagt tgccctgctg ttcagttccg
      541 tgtgaattat aaaaatggcg gtatttttta tcgctcagcg cgagatggtt acggattcga
      601 agctgactgg tcagaattct acaccacaac acgtaagcca tcagcgggag atgttggtgc
      661 atataccaaa gcagaatgta attcacgatt tattacaggt attcgattag gtacaaaatc
      721 gtcagtgcaa acatggaatg gtcctggttg gaacgataaa tctggttatg ttgttactgc
      781 ctcaatcaac tcaaataagg acgaacttat tgatacaact caggcaagac ctgtgcagta
      841 ttgtatcaat ggcacttggt ataacgcggg aagtatttaa ttatgcagca tttgaaaaac
      901 atcagatccg gaaatccaaa aactgttgaa caatatgaat tgacgcgaca attcggtgtt
      961 gtctggttgt attctgagga cgataaaaac tggtatgagg aacaaaagaa ctttcaggat
     1021 gacactataa aaattgctta cacatcagat ggaattattg tggctataga taaagacgta
     1081 tctacaataa atccagaagg tttaagcgtt gttgaggttg ctgacattac tgcaaatcgc
     1141 cgtgctgaca tttcgggtaa atggatgttc aaagatggcg tagtgataaa gcgaacttat
     1201 accgaggaag aacagaggca gcaggcagag aatgaaaaac aaagcctgtt gcaacttgtc
     1261 agggataaaa cccagctatg ggactcacag ctacggctgg gtatcatttc agacgagaat
     1321 aaacaaaaat taatcgagtg gatgctctat gcgcagaaag tcgaatctac agacacctcc
     1381 agcctgccag taacgtttcc cgaacaacca gaatgagaga aggcccgcta tcgggcctta
     1441 attttactct ggtttttgtg gccattctgg ctttgccgta tccacgcggc tgaccagaac
     1501 gctgtagcgt tcccaggctt ccagtcgtgt acgttcctca tctgttgcca tattcagcct
     1561 gacagcgcgc tccagtggct gaataacgct ttccgcttcg gaaagtaacg cggccttttg
     1621 tgattcggcc tgttgttgct gttcttctgc cgtataaatc cgtttaacca cagctccgtc
     1681 cttaaacatc cacttaccgg aatcatcggc ccgacgattg gctgttatat cggggacttc
     1741 aacaacgcta aagccttcag gattaagtgt ggaggcatct ttagtgatgg ctacaataat
     1801 attattttca tcgtaaacaa tctttattgt gtctggctga aaattcttta cctcttcata
     1861 ccagttcttc ccgtcttcag agtaaagcca gataactccg tgcttctttg ttaactcata
     1921 ctgctccggt gttttagcat taccaatttt tatgttctct aagtggatca tattaaacgc
     1981 tcgctacatt ataccaggtg ccatttatat acttttgaac gggtctgtaa taaacgccag
     2041 ctatattatc ggcagaattt gaccctgtat cctgaacatt tataccagac aatacatgac
     2101 ctgaagggca ctggaagttc catgtttgcc agttgttcac accataatat tgctgtgagc
     2161 caagccgaac atctttcaca tatcgggaat caatgttacc ccagttgctt ggttgtatct
     2221 gaccgtttac agcaaatatc accgagttat ctgtatttcg ctgactatag aaatgccagc
     2281 ctgaatcatc accaagctct gcaacagttg gtcttgacga atttccccat aagttaaatg
     2341 atgcattacg ggcagcggta tttgcacttg aaagtgtgaa gaacttactt tccccagcct
     2401 gaataccctt taatgatctt gctgaaccat ttataaagcg gaatacatgc acaccattag
     2461 cataagcatc cagcacccca tcaccatttt gtttaaaccc ggtatcgtta tctcctaaaa
     2521 ctattgaatt tccacccagg gcattagccg ttccaatccc cagatttccg ctaaaaatgg
     2581 cattaatacc gaatattgag tgagaacaat aaaaccctcg cccattgtta aaatccagaa
     2641 tggcttgtgc gttcgtgctt tctgtcgcgg gattagttgc tccccactta tatgttgttt
     2701 gaccgacggt ataatcctga gttggaacaa taaccgttag cccttcttca gcagaaaccc
     2761 gcacaggaaa agccctgcct tcaacgtaaa aaacgctgca tacatcatct tctttaaggc
     2821 ttgttacaat ggaatggatt gcgcgctcat tagtctgata cgtcgaaaaa taacctgccg
     2881 catatgaacc acgatcagtc cagccaccag gcattaccat tccattgaat tcgcagttat
     2941 tcatttcgta attaccaccg gcagatgtgg tcgttataat cactctggaa gccagctcgc
     3001 cagtgctgcc tgtggaacgc tggaaaataa ctggatacca ctttccacca acagcattag
     3061 acggcgcgtt aaatacgtac tgccgcattc cttttttctt atccacctcg cctttgctgt
     3121 aaacattaat gttactcagg aagcgatctt ta
//
LOCUS       DAZFWP020000290         3137 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFWP020000290 DAZFWP020000000
VERSION     DAZFWP020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.292"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(119..2026)
                     /locus_tag="ACHK0Z_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /pseudo
     CDS             complement(119..2026)
                     /locus_tag="ACHK0Z_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(2046..2507)
                     /locus_tag="ACHK0Z_RS25050"
                     /old_locus_tag="pgaptmp_005010"
     CDS             complement(2046..2507)
                     /locus_tag="ACHK0Z_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4504856.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(2535..>3137)
                     /locus_tag="ACHK0Z_RS25055"
                     /old_locus_tag="pgaptmp_005011"
     CDS             complement(2535..>3137)
                     /locus_tag="ACHK0Z_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504857.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRSTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT          602 a          908 c          835 g          792 t
ORIGIN      
        1 gagtatttga tcatgtataa gcaataaaaa acagcttcag gtaataagga atatctcaat
       61 ttttaaacat aaaatgcgaa ttatttagta caaaaaaccg gcgttacatg atctttccct
      121 ggctccacgt ctggtcgtta tcggaccagc ggatgatccg gtccgcagcg tcgtactcat
      181 aaaacaggtg gtagtccagc cgcgcatccg cttcggtcag ccgcccccgc gcatcctgtt
      241 cgtaggtcgc cagcgtctgt atgccgttat ccgtgcggtg aatggctttc aggtagccgc
      301 tggcgtggta cgtcagttta agccggatcc cgtcggtgtg actgacaccc gtgatccggt
      361 actggctgtc gcgctcaaac gtgatgcggt tgctggcggt gtcgctcatc ccgcccagca
      421 tgtacatccg gtcattgggg ctgtgtgcca ggcggtcatc gctggtatcg tcagtcaccg
      481 ggtggcgcag acgcattccc ggaaaggcgt cataaaagaa gcggctgctc agtgcatccc
      541 tgtcgaacag ctccagataa tcatcgcggc ggcgcagaat gtactgggcg tgatacgggt
      601 taaccgtcgt atcactgtca ccgtcgaagg caaatctcag cgtggctccc tggctgatga
      661 tatccacccg gtttccctgt tcacgcaccc aggcgtattc gctccaggag tcgctccagc
      721 ccacgcccgt caggccatgc agcggcagta cagaactgtg gaaacgggtg aaggagaggg
      781 ggagggtctg gcccagggtg aagtcggtac gttcgtcgca gtacgcgccg gttaccggat
      841 cgacaggatc tgttacccat ctgatgactt tattggcaat actttttaac gacttacctg
      901 ttttccccag caacgcgccg agctttgcgc ccagcagctt cccgcttttc cccagcaggt
      961 ttcccagttt gcccagtttc gccagattcc ccagttttcc cagcagtcct gctggcggca
     1021 gaagatagct ggctatcgtt attcctccca ggatcattct ctgccatggc gggaactccg
     1081 gctggatatc cagataggtg acctgctcac ccccaataaa gacgtccggc gaaccttcca
     1141 cgatcgccgc actgcactcc agtttgtcgc ccttgcgcgc ggcaggctga ttgttgataa
     1201 agacgttgct actgccctgg gctatcctcg gtggtttgtt ctccttgtcg cagagcgccg
     1261 tgtcctcacc ggcttccggg aaatccacct cttttttgat ttcgccacgc ccgcccacca
     1321 tggtggcaaa ctccattaat accggaccgg cgggaaacag aagcgatgcg ccggcgctga
     1381 cgcgatccat aaaaccggca ttgctttccg tcagggtgat aacagcgttc ccccaggcct
     1441 tcgccagtgg tactgctttt tccgctgccg attcaagcag gtcagtggca atatcacccc
     1501 atgagggttc cccctgtggg atttcaggta ttgctccagg tgggggagga gggggaaggg
     1561 tgacggcggc ccttgcagcg ggctttccgt taatgtttac attttcagaa ccggtattga
     1621 tggcgccgtc cgtgtcacaa aagctgttta tccatgattc aagctggttc tgtttctcct
     1681 ccacataatc actcgcctta tagctggcat acatcagcgc aggcaccaga accagcgcat
     1741 aagggccggt ggcgcaggcg gccataatca ggcaaccttc tgcgatggtc actatcgccc
     1801 ccagcacggc ccctgccagt gcccccagaa aacttttatg ctgaatttca tcgcccagcc
     1861 tggcggcagg tgggccggac ttctttgctg ccagcatctg tgcaatggct ttgtcgttgg
     1921 ctgcccgttt catctggctg gcgaccatcg cctgctgtga cagcattcgc attgccagcg
     1981 taccgcctgc ggtcgctccc tgtggcccgc ctggtccttc actcatgtgt tatccctcgt
     2041 ttatgtcagc gcggctggtc cttttggatg tcattctccg gtggcaacgg ggtgaagctc
     2101 tttatcacct ccagcagcat cgtcttttga ctgttcttca gggataagga ggtgctgaaa
     2161 gtgaaggtca gcagcgtatc tccacgccgc tggaatacgg ctatctggct aatccgcccc
     2221 tgattcccct catcttccgg accgttccag gaataatcca gcagccaggc cggggcctct
     2281 gccagagtga tttcccggca ggcatgttcg gtgtacccat caagtttgcc ctggtacagc
     2341 gccagcgttt cctggtacag ctctggtgtc agggttccct gtggaacggc ttcgcgggta
     2401 atgaccaggt tgatgccact gttatctgtg gacaccagga cctgcattga ctgatcctgc
     2461 cattgagacg gcagttgaat gtggccttca ttaaaagtga actgcataaa atactccctg
     2521 ataatctgag tgaatcagtt gatgaaaaca gaaccgccca cgatggtgat gccatccttc
     2581 gacagcctga ttaccgtcga ttccccgacc tgtagctgaa tgccatccgg cgtcagcgca
     2641 attttgcttt cctccacggc cagcaaaatt tccttaatcg ccgtataagc ctgtataccg
     2701 tcgccttcgc caccattgtt tacagcgtca acatgcaccg aaccggcaat cgtgcttctg
     2761 tcctgaaaga catcctcaga taaagaccct ttcagtactc cggtgatctt gtggcctttt
     2821 tccaccgtaa gaaggcggtt accttcctgg atcgtgaccg tctggtcatt ttttacggtt
     2881 tcgcggtgat cgtgttttac ggtggtggta cggtcgttga gcacttcggt atccatgttc
     2941 ttctgcgcgt ggatgtagac ctgttctctc cctgtcgcat catcaaactt cagctcatta
     3001 aatccgctgc ccatgtacgt tttggagcgg atggtcatct gcgtctttgt tcccggcagg
     3061 ctgccggggg tgcggttttc ctggtggtag gtgcgcccca taatgatcgg ctggtccgga
     3121 tcgccgttga ggaagtc
//
LOCUS       DAZFWP020000291         3107 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFWP020000291 DAZFWP020000000
VERSION     DAZFWP020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.293"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            58..1740
                     /gene="ydeN"
                     /locus_tag="ACHK0Z_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             58..1740
                     /gene="ydeN"
                     /locus_tag="ACHK0Z_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4504858.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1792..2949
                     /gene="ydeM"
                     /locus_tag="ACHK0Z_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             1792..2949
                     /gene="ydeM"
                     /locus_tag="ACHK0Z_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4504859.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2915..3037)
                     /locus_tag="ACHK0Z_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             complement(2915..3037)
                     /locus_tag="ACHK0Z_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504860.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          943 a          670 c          630 g          864 t
ORIGIN      
        1 cttatttctc ttcgtaaaat tacttcggaa ttaagtacaa taagaagagg aacatttatg
       61 aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt
      121 atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac
      181 gttgctttct cagactttac gccgactgaa tacagtacca aaggaaagcc aaatattatc
      241 gtactaacca tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac
      301 ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc
      361 attgaagctg cacaaaaatc aacgccgacg ctcctttcat taatggatga aggcgtacgg
      421 tttactaacg gctatgtggc acacggtgtt tccggcccct cccgtgccgc aataatgacc
      481 ggtcgagctc ccgcccgctt tggtgtctat tccaataccg atgctcagga tggtattccg
      541 ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac tgcagcagta
      601 ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacgcgt
      661 gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca aaaccgtggc
      721 tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcactg
      781 ttcaaaaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag
      841 gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct ttacctggct
      901 tataatgctc cgcacctgcc aaatgataat cctgcaccgg atcaatatca gaagcaattt
      961 aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt tgatcagggt
     1021 gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac aattattctc
     1081 tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa
     1141 ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa
     1201 ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccgaca
     1261 gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg cgtttccttg
     1321 ctgccctggt tgcaagataa gaaacaaggc gagccacata aaaatctgac ctggataacc
     1381 tcttattctc actggtttga cgaggaaaat attccattct gggataatta ccacaaattt
     1441 gttcgccatc agtcagacga ttacccgcat aaccccaaca ctgaggactt aagccaattc
     1501 tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa caatcagtta
     1561 ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag
     1621 gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca gccaccgctt
     1681 agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga agcgaaataa
     1741 ctaaaccttc atgcggcgga attttccgcc gccttattga gcgagatagc gatgcacgtt
     1801 acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa
     1861 aaagagtcgc agtttactca tgaaaaatgg atggatgaca gtactctgaa agagttcatc
     1921 aaacaatata tcgcagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc
     1981 actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg ctatgcaggc
     2041 caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa tgaatggtgt
     2101 gcctttctca aagaacatga atttctggtt ggtatctcga tcgatggccc ccaggagtta
     2161 catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt gatagcagcc
     2221 atcgagcgtc tgaaatcata tcaagttgag tttaatacgt taaccgtcat taataacgtt
     2281 aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag taaacatatg
     2341 caatttatcg aattgctcga aaccgggacg ccgaatattg atttcagtgg tcatagtgag
     2401 aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca
     2461 accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa
     2521 agctttgtca gccgtttttt ggggaatggg catacaagtt gtattttcca ggagtcctgc
     2581 aaggataatc tggttgttga aagtaatgga gacatttacg aatgcgacca ttttgtctat
     2641 ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa
     2701 ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata taaacctatc
     2761 tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat
     2821 ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa cgccatggtg
     2881 gagttagcta agaacagagt accgctttac cacattatgg atgttgcaaa acaaatggag
     2941 aataattaat aacgctctct tttaactcca ttcttagcct ccttcttatg accttaacaa
     3001 ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc
     3061 ctggcttacg tggtatgcgt cagggcatta gcaataccct tgattat
//
LOCUS       DAZFWP020000292         3068 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFWP020000292 DAZFWP020000000
VERSION     DAZFWP020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.294"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(51..1910)
                     /gene="gss"
                     /locus_tag="ACHK0Z_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             complement(51..1910)
                     /gene="gss"
                     /locus_tag="ACHK0Z_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU4504861.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2115..2981
                     /gene="yghU"
                     /locus_tag="ACHK0Z_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             2115..2981
                     /gene="yghU"
                     /locus_tag="ACHK0Z_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU4504862.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          718 a          828 c          780 g          742 t
ORIGIN      
        1 ccccgtactg attattcttc ataatcagta cggggttgct acaacatgta ttactttttc
       61 accacaatta acggttcaat atcactctct tttttgatga ccagtgattc atcaccgcgc
      121 aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac atacctgaat gtatttaccg
      181 tccactttcg gcaaacacca cagttgctga tagatgtttt tctgctcggc aaatttaccg
      241 ctggttttgt ccagcacctc ttcatgatgg ctgacgaggt cgatattgct gccacagcga
      301 ccggcgatcg gtttcactgc gtaacctgtt ttcaccagtt catcattaac agtgaaatcg
      361 gtatccagca ggtaacgatg gtgcgggaac agcgaccaga ggatcggcag aatcgctttg
      421 ttgccgggga tcaccgtcca cagcggctca aagaccagca cttccgggcg cagcaatacg
      481 tcgataagac gcacttcgtt ttgcggatga ccggtacgga ttggcaccgc agcaaactca
      541 cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc acgcccaggt tttccacacg
      601 cagttaacca gtcgcccttc cccatcaatc agttgcccgg cagcatccca gcccagttca
      661 tccagcccgc gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg ctccataaac
      721 tgcgcgtgat agttttcctc gatatctttg tcctgcatga tatggacaaa cggacgtgca
      781 cgactgtgtt tccaggcacc agccagttcg ttaatcagcc cttccgccgg attgaagccg
      841 ttgcctttat agccctgctc cgcccaacgt tcgaggatca agcccgcttc ggtatgacat
      901 gaggcagagt cggcgttgta ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc
      961 atacgaccgg tgatcatatg gtgacggcga cgctgccagg agagacgcaa acgtggccag
     1021 aggattttcg ggatgtcgaa cagcgccagc aggttgtcat ctttcagcac cttgtcggtt
     1081 gcgtgaagat acatcaggtg cagctcgttg gtggctttaa ttagctcctg ctcggcactc
     1141 tcggtaatgg tgtagtaatg ataaggatcc tgattgatca cctgaccgtt ggcctgcaca
     1201 taggcgtttt gcagcggatc tttttcatcc agccatttac cgtcaaactg gcctttgttt
     1261 tccaggcgcg ctccgctgat tttcagcagc tcgcctgcaa tttccggctg cggtaagctg
     1321 tattcagtat cttccgtctg gatcatccag cccagaatgg tggtgtcatc aaaagtgtct
     1381 ttcagggtat agcagccgtt ttcgaccacc atctccagct cacgcgtcca ctgttgccct
     1441 tgcggcaacg gggaatgaat cacgttctgt tccgcaatac ggactttgtt gccatgcaat
     1501 tgggtaatga tggcgacatg gccagtgtct ttaaattcac cgcctttatc ccagataaga
     1561 agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg
     1621 ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa tctcccacgc catacccacg
     1681 tcagtaaaga ccacaccgta attcagaaag agaaaacggc gagcaaattc aacgcattgc
     1741 cacttgtggc ccatatattc gtcgtcgata tagctacgga atacggcgtc atcttcgtat
     1801 tcctgcggat cgagagaact gtaatctgaa gagtagattg ctaccccacc tggggcgtag
     1861 cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat cactttacct
     1921 ttaacaatac aacctaagca gttggcgtaa ttgttgtgct ctgattacct gctcagcgac
     1981 attaaccgga cagaggtcag actaatcata cacctcatcg cagcggctgg cgcaaagggt
     2041 taaaaaattc acattctgta cagcaagtga cctgctacac tgcttcaaca ctaccactca
     2101 gaaggcaact cactatgaca gacaatactt atcagcccgc gaaagtctgg acgtgggata
     2161 aatccgctgg cggcgcgttc gccaatatca atcgcccggt ttctggtccg acgcatgaaa
     2221 aaacgctgcc cgttggcaaa cacccattgc aactttattc gctgggaacg ccgaacggtc
     2281 agaaagtaac gattatgctt gaggagctgc tggcgctggg cgttactggt gcagagtacg
     2341 acgcctggct gattcgtatt ggcgatggcg atcaattctc cagcggcttt gtcgaagtga
     2401 acccaaactc gaagatcccg gcgctgcgcg atcatacgca taatccgccg atccgcgtgt
     2461 ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt tggctacttc ctgccgcagg
     2521 atttggcaaa gcgtactgaa acgatgaact ggctgttctg gttacagggc gcggcaccgt
     2581 tcctcggcgg tggttttggt cacttttacc attacgcgcc gatgaaaatt gagtacgcca
     2641 tcaaccgctt taccatggaa gccaaacgtc tgctcgacgt gctggataag caactggcgc
     2701 agcataagtt tattgcgggc gatgagtaca ccattgcgga tatggcgatt tggccgtggt
     2761 ttggcaatgt ggtgttaggc ggcgtgtatg atgccgcaga gtttcttgat gcgggcagtt
     2821 ataagcatgt acaacgctgg gcgaaagaag taggcgaacg tccggcggtg aaacgtgggc
     2881 gtattgttaa ccgcaccaac ggaccgctga atgagcagtt gcatgagcgc catgacgcca
     2941 gtgatttcga gacgaatacg gaagataagc gtcaggggta agggttggtg ttcgtcgcag
     3001 caagccatcc aggccggata aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg
     3061 atgcgacg
//
LOCUS       DAZFWP020000293         3066 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFWP020000293 DAZFWP020000000
VERSION     DAZFWP020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.295"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK0Z_RS25085"
                     /old_locus_tag="pgaptmp_005017"
     CDS             complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK0Z_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor YmfM"
                     /protein_id="HGU4504863.1"
                     /translation="MNSLTTHYRRSQLIALPVPGGKAKVEYCYAVNVPGDREIVTHSF
                     AEWAVGDFNRQKETVLCDKLTAGSKITTE"
     gene            complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK0Z_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK0Z_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4504864.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSRQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(806..1006)
                     /locus_tag="ACHK0Z_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     CDS             complement(806..1006)
                     /locus_tag="ACHK0Z_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4504865.1"
                     /translation="MTTDDIESYFGSIEKVAAFFGITTEAVYQWRNRPGQLIPKGRAA
                     EAAYRTCGRLPFKPELYEKSNG"
     gene            1104..1730
                     /locus_tag="ACHK0Z_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             1104..1730
                     /locus_tag="ACHK0Z_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4504866.1"
                     /translation="METVGQRIKALRRVTGTSQKELGKFCGVSDVAVGYWEKDINTPG
                     GEALSKLAKFFNTSIDYILYGAEFEGKLVTNMRRVPVISWVQAGQFTECRAAEVFSEV
                     DKWVDTSLKIGDNSFALEVKGDSMTNPNGLPTIPEGATVIVDPDAEPRHGKIVIARLD
                     GTNEATVKKLVIDGPQKFLVPLNPRYPNIPINGNCLIIGVVKGVQYEL"
     gene            1937..2212
                     /locus_tag="ACHK0Z_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             1937..2212
                     /locus_tag="ACHK0Z_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504867.1"
                     /translation="MKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIMAGVVFSL
                     RRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            2488..2798
                     /locus_tag="ACHK0Z_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /pseudo
     CDS             2488..2798
                     /locus_tag="ACHK0Z_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2813..>3066
                     /gene="yfdP"
                     /locus_tag="ACHK0Z_RS25115"
                     /old_locus_tag="pgaptmp_005023"
     CDS             2813..>3066
                     /gene="yfdP"
                     /locus_tag="ACHK0Z_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4504868.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETP"
BASE COUNT          839 a          710 c          711 g          806 t
ORIGIN      
        1 actccgtagt gatctttgaa ccagcggtta acttgtcgca aaggactgtc tccttctgcc
       61 ggttgaaatc acccacagcc cactcagcaa agctgtgggt tacaatttcc ctgtcacctg
      121 gtacattaac tgcatagcaa tactccacct tcgcttttcc acccggtaca ggaagcgcaa
      181 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacagtc
      241 acgacacgcc acggcgcccg gagctgcaca ctcgcgggcg tcattacttt ctgaaatgca
      301 aaagattttg tagaccagtg ctgcatgctc ctgcagcttc gaaattgaga gatacagctc
      361 gtcgttaatt gctgtcttct catgcggttc caccacaccg tcttcgattg ccgaacgaat
      421 ctgtctggaa taactgccaa tctgttcaat gacttccagc aggcgctggt taatatcggc
      481 gttgtccaca tcctcgacgt caggaagaga cacaaagacg ccatttgcag actgcgccac
      541 agcgtcagca atgaagtgag ttccaccagc acgttgcaaa atcattgccc atcccagcgg
      601 gaaaatctga tcgccatcgg cacgaaggcg gttaaataat gcgttttctg ttacatccag
      661 ccagtcagct gcttcagcgt aaccacccgg caacgctgcg atagtttttc tgacagcttt
      721 cacgtaccac tcaggctgtt tttctacttt ccagtgatgc ttacccacgg ttagcctcat
      781 cgttctgtgg tttctgttaa tcgatttatc cattagattt ttcataaagc tcaggtttaa
      841 atggcaaccg tccgcaagtt ctatatgcag cttctgctgc acgtcctttt ggaattaact
      901 ggcccggacg gtttcgccac tgataaacgg cttcagttgt tatgccgaaa aaagcagcaa
      961 ctttctcaat actgccgaag tagctttcga tatcgtcagt tgtcatacgc cctccaaact
     1021 aagttttatt agatgctaat tacaaatcta tctttggtca ataaaaacta agattactta
     1081 gcaattcaag aaatggtgct cctatggaaa cggttggtca gcgtataaaa gctctgagaa
     1141 gagttaccgg aacgtcccag aaagaattgg gtaaattttg tggagtaagc gacgttgctg
     1201 tggggtactg ggagaaagac atcaataccc ctggtgggga ggcactttcg aaattagcga
     1261 agttcttcaa tacgtcaata gattacattc tttatggtgc tgagtttgaa ggcaaactcg
     1321 tcacaaacat gcgcagagtt cctgtaatat cgtgggttca ggctgggcag tttactgagt
     1381 gcagggcagc agaagtgttt agtgaagtgg acaagtgggt agatacatca ttaaagattg
     1441 gtgataactc atttgcatta gaggttaaag gtgactccat gactaaccct aatggcctcc
     1501 caacaatacc agaaggcgca acagtgattg tagatccaga tgcagaacct cgtcatggaa
     1561 aaatagtcat cgctcgactt gatggaacaa acgaagctac agtaaaaaaa ttagtcatcg
     1621 atggccctca aaagttttta gtgccattaa atcctcggta tcccaacatc cctatcaatg
     1681 gtaattgcct tatcattggt gtagtcaaag gagttcaata cgaactctaa gacctctctt
     1741 ctctaactaa ggcaccgaac taagaaaagt ttggtgtttt ctcttgccat aataactaag
     1801 ttaagttaga ttttatatca aagataacga acaggcagga cgcccacgaa gtagccgcct
     1861 ggggcatatg aagtccagga tgattcgttg agtcatgttg tgccatcagg cactcatgtt
     1921 aaagcaggtg tatgaaatga aagtccagat tttaaacaat aactgtgaag tcgtttggtc
     1981 atacgacata gccgcccctg tagatcagag cggcgatagc tggaccaatg ggaaacatca
     2041 gattatggct ggagttgtgt tctctttacg ccgtgctttg gaacaggctg aagtatttcc
     2101 atcagaccct gaatggaaat ggcctttttc tatttgtcca aattcggaga gcacatttca
     2161 gaaaattggt cagaaagtcg cactcgaaga gcatcagcca actgtttcct gattttttca
     2221 ggtaactcgt cggcatcgca gaaacaacaa cgctcgatca tgttgaaagc cgattcgtag
     2281 aaccgtttct gctgagtgtc gctgagacag gaaaagagtg acgttacgat gattttatta
     2341 attgcattat caagttcttt ttcatcaaaa gtcatttgat tttcctttta tgtatacggg
     2401 cttaaaagga taccaccgag cctgaagtgg tgaaaagaca ggcacataac agctaagtat
     2461 tttcaaccaa agagaatcct tagcgttgtg gtgaatgcgg ctcagcgcac gcgggttaag
     2521 gttgaggctg acagtcgacc ttctgtggat acccacccgt ctggtgtgca accttcgcca
     2581 ggcaccggga ggcacccggc accacaactt tatgctgtgt gtagtcctgg cggtaccagt
     2641 ttgtaccctt gcttccggct ggtagcgtcc tttttacaaa acagagaaga gcatcaccgg
     2701 acgacgggct cataacccaa tccatccggg cggctgccac cgcaggtgtt cttctctgtt
     2761 ttgtggagaa actaatcggc cttgcagggt cgatatgatg aggagcagca aaatggctag
     2821 cgaacgcagt actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac
     2881 caaaatcggc gcagttctca gtgaagtcgc ttccggtgtg atgaacacga aaaccaaagg
     2941 taaggtctca ctcaacctgg aaatcgaacc atttgatgag aaccgtgtga aaatcaaaca
     3001 caaactctca tatgttcgcc cgactaaccg cgggaaaatt tctgaagaag acaccaccga
     3061 aacgcc
//
LOCUS       DAZFWP020000294         3004 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFWP020000294 DAZFWP020000000
VERSION     DAZFWP020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.296"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..210)
                     /locus_tag="ACHK0Z_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             complement(<1..210)
                     /locus_tag="ACHK0Z_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4504869.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRI"
     gene            complement(243..536)
                     /locus_tag="ACHK0Z_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             complement(243..536)
                     /locus_tag="ACHK0Z_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGU4504870.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(656..871)
                     /locus_tag="ACHK0Z_RS25130"
                     /old_locus_tag="pgaptmp_005026"
     CDS             complement(656..871)
                     /locus_tag="ACHK0Z_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4504871.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            975..1607
                     /locus_tag="ACHK0Z_RS25135"
                     /old_locus_tag="pgaptmp_005027"
     CDS             975..1607
                     /locus_tag="ACHK0Z_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4504872.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGNGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            1697..2008
                     /locus_tag="ACHK0Z_RS25140"
                     /old_locus_tag="pgaptmp_005028"
     CDS             1697..2008
                     /locus_tag="ACHK0Z_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504873.1"
                     /translation="MNGDRYEFTKDSTEDSVFNVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVETWSITTDKKALYSKVMNIPGMQQLTSTKSFVGDVVGTCN
                     Q"
     gene            2228..2683
                     /locus_tag="ACHK0Z_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             2228..2683
                     /locus_tag="ACHK0Z_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU4504874.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTDRRIQ
                     MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKPKSRVESALNPIDLT
                     VLAEYHEQIESNLQRIERKNHRVWYSKPGEFGITCQGRQKVKGESIPLA"
     gene            2692..>3004
                     /locus_tag="ACHK0Z_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             2692..>3004
                     /locus_tag="ACHK0Z_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504875.1"
                     /translation="MKKIDYKSIPKPIDSASERKKHKKEAEKLANYISFIRNNAHGDG
                     DKKLLSDARTQAFGILRKQMQYRLHPGYIIEIRPTERQLFLLNSVFDFVNVVGDLIDR
                     SV"
BASE COUNT          869 a          653 c          673 g          809 t
ORIGIN      
        1 gattctgtcc attggtttat tccacccata ggttttacgc agaatggcaa gcagcacttt
       61 aaactgtcgc ttggtcagat ctgcgcctga ataagcctca agcagcatat ttgatagtct
      121 ggcgtaacca tcatcgagat ctgccacatt acgctcctgt ccggcaaagt tacctctgcc
      181 gaagttgagt atttttgctg tatttgtcat aatgactcct gttgatagtt ccagtaataa
      241 cctcagaatt ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
      301 gtgagaatcg aagcaacttg tcgtgccaat cgagccatgt cgtcgtcgac gacaccccat
      361 tcaagaacag caagcagcat tgagaacttt ggaatccaat ccctcttcca cctgctgatc
      421 tgcgacttat caactcccac agcttccgct gtcttctcag ttccaagcat tgcgattttg
      481 ttaagcaacg cactctcgat tcgtagagcc tcgttgcgtt tgtttgcacg aaccatatgt
      541 aagtatttcc ttagataaca attgattgaa tgtatgcaaa taaatgcata caccataggt
      601 gtggtttatt ttgatgccct ttttcagggc tgggatgtgt aagagcggga atgtcttaag
      661 cggctttacc gcgtttagtt ccgtactgta accaaaccgg atcacagtta agcgccatag
      721 caatctcaaa caagaagcgc ggtcgcttgg ttactccagc ttcaatcagt tgaattgatt
      781 gctgtttaac accggctttg gttgccagtt cggtttgcgt catttttaac gcaattcgcc
      841 tcttcttgag gcgttcagaa agagtttgca tatcgcctcc atcaacaaac tttcttgtat
      901 tttcatacaa tgtatcttgt ttgtcaaata cagtttttct tgtgaagatt gggggtaaat
      961 aacagaggtg gcttatgagt atttcttcca gggtaaaaag caaaagaatt cagcttggac
     1021 ttaaccaggc tgaacttgct caaaaggtgg ggactaccca gcagtctata gagcagctcg
     1081 aaaacggtaa aactaagcga ccacgctttt taccagaact tgcgtcagct cttggcgtaa
     1141 gtgttgactg gctgctcaat ggcacctctg attcgaatgt tagatttgtt gggcacgttg
     1201 agcccaaagg gaaatatcca ttgattagca tggttagagc tggttcgtgg tgtgaagctt
     1261 gtgaacccta cgatatcaag gacattgatg aatggtatga cagtgacgtt aacttattag
     1321 gcaatggatt ctggctgaag gttgaaggtg attccatgac ctcacctgta ggtcaaagca
     1381 tccctgaagg tcatatggtg ttagtagata ctggacgcga gccagtgaat ggaagccttg
     1441 ttgtagccaa actgactgac gcgaacgaag caacattcaa gaaactggtc atagatggcg
     1501 gtcagaagta cctgaaaggc ctgaatcctt catggcctat gactcctatc aacgggaact
     1561 gcaagattat cggtgttgtc gtggaagcga gggtaaaatt cgtatgatca ggattgcggc
     1621 gctactctca atactcttaa ctaccagcgc caattctgaa tgctggattg tcacaaacct
     1681 gcacgggtac ggggcaatga atggcgatcg ttacgagttt acaaaagaca gcacggaaga
     1741 ttccgttttc aacgtaacaa taaatggcga taaatcatca gtttatgaat cagtttctgg
     1801 cgtctatcca gagatgaaat acactgcttt gtcatcgaac actatggtag gagaatacca
     1861 gtctggagga ggcataaccg ttgaaacttg gtcaatcact acagacaaaa aagctcttta
     1921 ctccaaagta atgaacatcc caggtatgca acaacttaca tcaaccaaat cctttgttgg
     1981 tgatgtagtc ggaacctgca atcagtaatc cccacctcaa tctcgataac aaaaaaacaa
     2041 actattttcc gtttaaaaac aatggagttt gtttttcacg ccctttttta caatatttct
     2101 tgtttacaac atacaatctt ccttgtaatt ttaagccatc agcaggacgc actgaccacc
     2161 attgaaggtg aggctcttaa aaattaagcc ctgaagaagg gcagcattca aagcagaagg
     2221 ctttggtgtg tgtgatacga aacgaagcat tggccggaag tgcgaatccg gattagctgc
     2281 caatgtgcca ttgcggggtg ttttcgttca ggactacgac tcccacacac aaccaaagct
     2341 aactgacagg agaatccaga tggatgcaca aacacgccgc cgcgaacgtc gcgcagagaa
     2401 acaggctcaa tggaaagcag caaatcccct gttggttggg gtaagcgcta aaccagttaa
     2461 ccgccctatt ctctcgctga atcgcaaacc gaaatcacga gtagaaagcg cactgaatcc
     2521 gatagacctt acggtgctgg ctgaatacca cgaacagatt gaaagcaacc tgcaacgtat
     2581 tgagcgcaag aatcatcgag tttggtatag caagccaggt gagttcggta taacttgtca
     2641 aggaagacaa aaggttaaag gggaatccat tccattggca tgaggtacgt aatgaagaag
     2701 attgattaca agtccatacc aaaaccaata gactcagcat cagagcgaaa aaaacacaaa
     2761 aaagaggctg aaaaattagc aaattatatc agttttatta gaaacaatgc tcacggcgat
     2821 ggcgacaaga agttgctttc agatgctcgg acccaagcgt tcggtatact tcgtaagcag
     2881 atgcaatatc gtcttcatcc tggctacata attgaaattc gccctactga aagacaattg
     2941 ttcttattaa actctgtctt tgactttgta aacgtagttg gagatcttat cgacaggtct
     3001 gtag
//
LOCUS       DAZFWP020000295         2971 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFWP020000295 DAZFWP020000000
VERSION     DAZFWP020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.297"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(133..209)
                     /locus_tag="ACHK0Z_RS25155"
                     /old_locus_tag="pgaptmp_005031"
     tRNA            complement(133..209)
                     /locus_tag="ACHK0Z_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Asp,seq:gtc)
     gene            complement(342..1073)
                     /gene="dnaQ"
                     /locus_tag="ACHK0Z_RS25160"
                     /old_locus_tag="pgaptmp_005032"
     CDS             complement(342..1073)
                     /gene="dnaQ"
                     /locus_tag="ACHK0Z_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU4504876.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1138..1605
                     /gene="rnhA"
                     /locus_tag="ACHK0Z_RS25165"
                     /old_locus_tag="pgaptmp_005033"
     CDS             1138..1605
                     /gene="rnhA"
                     /locus_tag="ACHK0Z_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU4504877.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1602..2324)
                     /locus_tag="ACHK0Z_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             complement(1602..2324)
                     /locus_tag="ACHK0Z_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4504878.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2358..>2971
                     /gene="gloB"
                     /locus_tag="ACHK0Z_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             2358..>2971
                     /gene="gloB"
                     /locus_tag="ACHK0Z_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4504879.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPV"
BASE COUNT          807 a          722 c          695 g          747 t
ORIGIN      
        1 ctaccatatg tgattaacac atttacagca taacaaaacc atagaccgaa gaaatagcgg
       61 gatttaaatg caaaaaaaaa ccgcaaaatt aaaaatcttg cggttctctg aactcatttt
      121 catgagtgaa tttggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca
      181 ggtattctaa ccgactgaac taccgctccg cgttgtgttc cgttgggaac gaggcgaata
      241 ttacggattg cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa
      301 aaatcgccca agccgctatt ttcagcgcct tttacaggta tttatgcccg ccagaggcag
      361 cttccgccct tcttctccac cagatcaaga cgggcttcat gagctgcaag ctcttcatct
      421 gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa tgcgctgaat tgttgcttca
      481 ccttgttgct gctgtgtctc tccttccatc gcaaaagcca tcgacgtttg accaccggtc
      541 atcgccagat aaacttccgc aaggatctgt gcatcgagta atgccccgtg tagcgttcgt
      601 ttactgttat ctatttcgta gcgggcacat aacgcatcga ggctgttgcg cttaccagga
      661 aacattttcc tcgccaccgc aaggctatcg gtgaccttac agaaggtatt ggtcttcgga
      721 atatcgcgct taagcaacga aaactcgtag tccataaagc cgatatcgaa cgctgcgtta
      781 tggatcacca actccgcgcc gcggatatag tccatgaact catcggctac ttcggcaaac
      841 gtgggcttat cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc ttccggatcc
      901 accagccgat cgggtttgag ataaacatgg aagttattgc ccgtcaggcg acggttcacc
      961 acttcaacgg caccaatctc aatgatcttg tggccttcat agtgcgcacc aatctggttc
     1021 ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc
     1081 atttatgtca gacttatcgt tttacagttc gattcaatta caggaagtct accagagatg
     1141 cttaaacagg tagaaatttt caccgatggt tcgtgtctgg gcaatccagg acctgggggt
     1201 tacggcgcta ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc
     1261 accaccaaca accgtatgga gttgatggcc gctattgtcg cgctggaggc gttaaaagaa
     1321 cattgcgaag tcattttgag taccgacagc cagtatgtcc gccaggggat cactcagtgg
     1381 atccatagct ggaaaaaacg tggctggaaa accgcagaca aaaaaccagt aaaaaatgtc
     1441 gatctctggc aacgtcttga tgctgcattg gggcagcatc aaatcaaatg ggaatgggtt
     1501 aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg aactggcccg tgctgcggca
     1561 atgaatccca cactggaaga tacaggctat caagtcgaag tttaagcctg tggtttacga
     1621 tattgccggg tggctccaac cgcctggcga attcgtggct tgtttttact ctgtttcatc
     1681 ggatttagcg ttaaaggaat agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc
     1741 gcaggaatat gtgcattcaa tagttttcca ccgtgtttgt tccacgggag aacgtggaaa
     1801 cggctggcgt gcagcacttc aaaattcaac aaagagagcc agtccagttg ccgcatcaga
     1861 gtaaacatcc ggctgttata gggcgaggtt ttgcgcaata ccggcacaag tttgcgtaat
     1921 cccattaaac tgatgggatt gaagccacta atcaccagcc agccatcatc aatcaatacc
     1981 cgatcggctt cacgcaataa acgatgcgga tcggtgcacc acggcaatgt atgtgccagt
     2041 agacaaacat caacggattt atcggcaaaa ggaagatgaa gtgggtccgc ctgtacctgg
     2101 acgggcattc cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg
     2161 atttctgcgc ttaaattgcc aatcttaagc agatgaaaac catacatttt agtgaaccac
     2221 gggttgagct ggcgctccag cgccttgcga taaagctctc cccagggcaa atcgccccag
     2281 caatcaggag ccacgacagt ttgagggact cttgccggtt tcatcacaac cttccgtttc
     2341 acactaagag gtaatctatg aatcttaaca gtattcccgc ctttgatgac aattacatct
     2401 gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga tcccggagac gcagagccag
     2461 tattaaacgc cattaccgcc aataactggc aaccggaggc catatttctc acccaccatc
     2521 atcacgatca cgttggcggc gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt
     2581 atggtccaca agagacacaa gataagggaa caacacaggt agtcaaagat ggcgaaactg
     2641 ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc gggtcacact ttaggacata
     2701 tctgttactt cagtaaacct tatctatttt gcggcgacac gctgttttct ggtgggtgtg
     2761 gtaggttgtt tgaagggaca gcatcacaaa tgtatcaatc acttaataag ttaagtgcgt
     2821 tacctgacga tacattggta tgttgtgctc atgaatatac gttatcaaat atgaagtttg
     2881 ctttgagtat tcttccgcac gatttgtcca taaatgatta ttatcgtaaa gttaaggagt
     2941 tacgggcaaa aaatcaaata acactacccg t
//
LOCUS       DAZFWP020000296         2919 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFWP020000296 DAZFWP020000000
VERSION     DAZFWP020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.298"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            289..1455
                     /gene="nhaA"
                     /locus_tag="ACHK0Z_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             289..1455
                     /gene="nhaA"
                     /locus_tag="ACHK0Z_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU4504880.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1521..2420
                     /gene="nhaR"
                     /locus_tag="ACHK0Z_RS25185"
                     /old_locus_tag="pgaptmp_005037"
     CDS             1521..2420
                     /gene="nhaR"
                     /locus_tag="ACHK0Z_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU4504881.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2459..>2919)
                     /locus_tag="ACHK0Z_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             complement(2459..>2919)
                     /locus_tag="ACHK0Z_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4504882.1"
                     /translation="YNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFD
                     CIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNE
                     IQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          705 a          639 c          717 g          858 t
ORIGIN      
        1 attatttttg aaatattggc ctaacaaaca tcgccgactg acaacaaatt aattattact
       61 tttcctaatt aatctctcag aaatcctcac cttaagctat gattatctgg gcttagggtc
      121 actcgtgagc gcttacagcc gtcaaaaact catctcaccg ctgatggcgc aaattcttca
      181 atggatcgta aaaaacgagt tattcctaca ctataatctg attttaacga tgatttgtgc
      241 ggggtaaaat agtaaaaacg atctattcac ctgaaagaga aataaaaagt gaaacatctg
      301 catcgattct ttagcagtga tgcctcggga ggcattattc ttatcattgc cgctatcctg
      361 gcgatgatta tggccaatag cggcgcaacc agtggatggt atcacgactt tctggagacg
      421 ccggttcagc tccgggttgg ttcactcgaa atcaacaaaa acatgctgtt atggataaat
      481 gacgcgctga tggcggtatt tttcctgtta gtcggtctgg aagttaaacg tgaactgatg
      541 caaggatcgc tagccagctt acgccaggcc gcatttccag ttatcgccgc tattggtggg
      601 atgattgttc cggcattact ctatctggcc tttaactatg ccgatccgat tacccgcgaa
      661 gggtgggcga tcccggcggc tactgacatt gcctttgcac ttggtgtgct ggcgctgttg
      721 ggaagtcgtg ttccgttagc gctgaagatc tttttgatgg ctctggctat tatcgacgat
      781 cttggggcca tcattatcat cgcattgttc tacactaatg acttatcgat ggcctctctt
      841 ggtgtcgcgg ctgtagcaat tgcggtactc gcggtattga atctgtgtgg tgtacgccgc
      901 acgggcgtct atattctggt tggcgtagtg ttgtggactg cggtgttgaa atcgggggtt
      961 cacgcaaccc tggcgggggt aattgtcggc ttctttattc ctttgaaaga gaagcatggg
     1021 cgttctccag cgaagcgact ggagcatgtg ttacacccgt gggtggcgta tctgattttg
     1081 ccgctgtttg catttgctaa tgctggcgtt tcactgcaag gtgtcacgct ggatggcttg
     1141 acctccattc tgccattggg gatcatcgct ggcttgctga ttggcaaacc gctggggatt
     1201 agtctgttct gctggttggc gctgcgtttg aaactggcgc atctgcctga gggaacgact
     1261 tatcagcaaa ttatggcggt ggggatcctg tgcggtatcg gttttactat gtctatcttt
     1321 attgccagcc tggcctttgg tagcgtagat ccagaactga ttaactgggc gaaactcggt
     1381 atcctggtcg gttctatctc ttcggcggta attggataca gctggttacg cgttcgtttg
     1441 cgtccatcag tttgacagga cggtttaccg gggagccata aacggctccc ttttcattgt
     1501 tatcagggag agaaatgagc atgtctcata tcaattacaa ccacttgtat tacttctggc
     1561 atgtctataa agaaggttcc gtggttggcg cagcggaggc gctttattta actccacaaa
     1621 ccattaccgg acagattcga gcgctggaag agcgcctgca aggcaaatta tttaaacgca
     1681 agggacgtgg tctcgaaccc agcgagctgg gagaactggt ctatcgctat gccgataaaa
     1741 tgttcacctt aagccaggaa atgctggata ttgtgaacta tcgcaaagaa tccaatttat
     1801 tgtttgacgt tggtgtggct gatgcacttt ccaaacgcct ggtcagtagc gtacttaacg
     1861 ccgcagtggt agaaggcgag cccattcatc ttcgctgctt cgaatccacc cacgaaatgc
     1921 tgctggagca attaagtcag cataaactgg atatgatcat ttctgactgt ccgatagact
     1981 ctacgcagca ggaaggcctg ttctccgtga gaattggcga atgtggcgtg agtttctggt
     2041 gtacaaatcc accaccagaa aaaccgttcc cggcttgtct ggaagaacgg cgacttttga
     2101 ttcctgggcg acgttcaatg ttagggcgca aattgcttaa ctggtttaac tcccagggat
     2161 taaacgtaga aatcctcggc gagtttgatg atgccgcgtt gatgaaagct tttggtgcga
     2221 tgcacaatgc aatcttcgtt gccccaacgc tttatgcata tgacttttat gccgataaaa
     2281 ctgtcgtaga aattggtcgc gtcgagaatg tgatggaaga gtaccatgct atttttgctg
     2341 agcggatgat tcagcacccg gcggtacagc gaatctgcaa tacggattat tctgcgcttt
     2401 ttagtccagc ggcgcgttaa tcggcacctc cccccaaagt taaggtgggg gagatagatt
     2461 agttgtacat aaccacgatt ttgactcggc tcattatttg cccgcttgag acattatttc
     2521 catatgtacg cgggcgaata aatagaggaa tctgattact tccttcatgg ggatgctgaa
     2581 aagagtagta attgctttta ccatactgga tttcattcgt accatcattg gagagttgaa
     2641 agcctatttg acccgcaccg tcctcactgt tttctaactc attttttaat atgctggttc
     2701 ctgactcctg tatcgtcact ggttcaaagt aatattcaac atcactgggt tcaaaaacta
     2761 attcaccttg ctcgccctgg atgcagtcaa atcttatatg atgaaactga taattttcat
     2821 tattgatacg agacagtaca aatcgtccag tgtcgatgga ttgatcttca aaaccacagg
     2881 tcattaaatt tacctgtaaa atagtattta aattataat
//
LOCUS       DAZFWP020000297         2917 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFWP020000297 DAZFWP020000000
VERSION     DAZFWP020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.299"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..230)
                     /locus_tag="ACHK0Z_RS25195"
                     /old_locus_tag="pgaptmp_005039"
     CDS             complement(<1..230)
                     /locus_tag="ACHK0Z_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4504883.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETL"
     gene            complement(246..725)
                     /locus_tag="ACHK0Z_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             complement(246..725)
                     /locus_tag="ACHK0Z_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4504884.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPLWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(807..1628)
                     /locus_tag="ACHK0Z_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             complement(807..1628)
                     /locus_tag="ACHK0Z_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4504885.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGKVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(1849..2259)
                     /locus_tag="ACHK0Z_RS25210"
                     /old_locus_tag="pgaptmp_005042"
     CDS             complement(1849..2259)
                     /locus_tag="ACHK0Z_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504886.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(2275..2907)
                     /locus_tag="ACHK0Z_RS25215"
                     /old_locus_tag="pgaptmp_005043"
     CDS             complement(2275..2907)
                     /locus_tag="ACHK0Z_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504887.1"
                     /translation="MARAGEYKNYSRDEIKYWRYTSYKGGKLPEGFTDEKFSSAIYNG
                     RIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEAWAEQPGACPSTYELYEG
                     DATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTVWFQGDGCKANTCGWD
                     FIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
BASE COUNT          680 a          740 c          732 g          765 t
ORIGIN      
        1 aagagggttt caccggcaat cagttgattc tggttattca gatacagcac ccggaattct
       61 tcacgctcca gtcccgccat gttcagaatc agccattccc gtgctgcacg ggtggaggtg
      121 aaggccacgc cgggttcatg aagatggcgg tccagggttt tcagggcccg ttgaatgagg
      181 ctgcgctcgc cgggcgtcat ctctccgggc agaaaggaaa gttgttgcat tgtgcttctc
      241 tccattcagt cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc
      301 cgcaaccggt aataatggac cgtcatggca taattctccg tacgacaggc atgatgactg
      361 tacgtcatca gacaggcggc aataccggcg gcttccgggc tcatttctgc gcggttaccg
      421 ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt catccggttc gggtgccata
      481 aatgccccgc cgttgttcag ggtgtacaga ttccagatac caccgcagta gtcttcgcac
      541 agacggtcca tccagccgaa gacacggggc tccagggtca cccacagtgg aatgaggcca
      601 aaatgctgcg gccagaagct gatgcgctgt tcatcaggga cgaggctggc aaccaactga
      661 ggcggattat tctctggtgt tgtagctgaa taaattgtgg gggtattctg agaaacggtt
      721 ttcatgagat aactccttaa ataacaaata aatgtgtgtt tttcaggaac gaaagctgaa
      781 tgattccctc actcagggaa acggcatcag cgcaggctct ccagcatcgt ttctgccatc
      841 acccacaatg cgcgattgag cttaatgtcg gtatcaatgc tgtgaatggc ccgggtatgg
      901 atacgttttc cttttgcact gcgaccggaa atcccgcctt tcagcatatt ctcctggatg
      961 gtctgatatg cgctccacag gtccttaccg taatcctccc ggcgtcgcgg cgtcagaatg
     1021 tcggcggtgg tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
     1081 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttctcctct
     1141 atccggtcaa acacgcccac cacctcgtaa gccccttcga taactttctc cactacattt
     1201 ccccggtgtg gaacacgcac tttccccaga gactggccac agacgcaccc gttctggcag
     1261 acgaacctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
     1321 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
     1381 tgttttgtgt atccccggcg gcccgggtcg cgcacacggg tctggcaggc gaagaacggc
     1441 tgaaagcctt cccgctgcag attttccagg acggtaatgg tgggaatgta cgcataccgt
     1501 tcactgcggg aggtgtgccg gtcttcccca aaaatactgg gtacatggcg tatcagttct
     1561 tcatgtgtca gcggacggtc acggcgtatc tggtttgcag cataaccaaa acgacttgct
     1621 aatcgcatac tttgctcctt atcggtggtt aagatttact ggtgcaataa atgaaaaaag
     1681 ccgcgtctcc cggagaagac gcggcctgac gggtgaaatg aataaggttt attgtctgag
     1741 aggcctttca ctggcgagcc acgcattaag cggctttccg gtaacaatgg ctggggctgt
     1801 atttaccttt gatattaata atgtgcagag aaacgcatgg tttactcctt agattttaag
     1861 tttgtcctgg gctacaacga caggttttcc ggatgtgtcg atgactccta ccgtcggtac
     1921 gccatttttg atttcgaaga acggtttgtt gctacctgag cacagttgat tgtccccgta
     1981 ttcaatcatt tgtgctttcc cggttttcag gttaactacc gagacagtgt agtcgtagca
     2041 ccaggcacca ccacgttcaa gcacgaaaac atagctgtta tctttatagt tgaactgatt
     2101 ctttgtctgg caattcatac cgtcaatttc ggtacaggaa aacgcttctg tagtattaac
     2161 gtatttgatg ttatttggca gttttacttg tttcgcagta gcgaagacag agaacagaat
     2221 agttacagca acgagtaaag ttttgataaa agttttcatg atagactcct taaattagtt
     2281 aaccgcagct acgtagtttt tcaccagata cgcgaattca tgcacgccaa tttcaccgaa
     2341 ccaaaccagg taggccgggt catctaaacc aaagtaacgg tagccaacaa cttcatgggt
     2401 ttttgggtta tagagtgtta cgataaagtc ccagccgcag gtattggctt tgcaaccgtc
     2461 gccctggaac cagactgtac cgttaatgtt tactggcgtg atacctccac cagggccatc
     2521 catatagccg cctttaccga acatgccgct cagtccgacc ggttttaatg ctttgtcgat
     2581 agcggctttg taggtagcgt caccttcgta caactcgtac gttgatggac acgctccagg
     2641 ttgttcagcc caggcttccg ggttacaact aagagcttgt tttacggtag ccgcttctgc
     2701 gttaaagtta aagcccgtga acagaaccgc cagaaataac ataagggtat gtaaacgttt
     2761 cattgtaaat attcttccgt tgtaaatggc gctggaaaat ttctcatcag tgaaaccttc
     2821 cggaagtttc ccccccttgt atgatgtata tcgccagtat tttatttcat cccggctgta
     2881 atttttatat tccccggcac gtgcaataac gctgacc
//
LOCUS       DAZFWP020000298         2909 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFWP020000298 DAZFWP020000000
VERSION     DAZFWP020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.300"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            31..507
                     /gene="agaB"
                     /locus_tag="ACHK0Z_RS25220"
                     /old_locus_tag="pgaptmp_005044"
     CDS             31..507
                     /gene="agaB"
                     /locus_tag="ACHK0Z_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU4504888.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            546..1349
                     /gene="agaC"
                     /locus_tag="ACHK0Z_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             546..1349
                     /gene="agaC"
                     /locus_tag="ACHK0Z_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU4504889.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAAIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1339..2130
                     /gene="agaD"
                     /locus_tag="ACHK0Z_RS25230"
                     /old_locus_tag="pgaptmp_005046"
     CDS             1339..2130
                     /gene="agaD"
                     /locus_tag="ACHK0Z_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU4504890.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2131..2886
                     /gene="agaI"
                     /locus_tag="ACHK0Z_RS25235"
                     /old_locus_tag="pgaptmp_005047"
     CDS             2131..2886
                     /gene="agaI"
                     /locus_tag="ACHK0Z_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGU4504891.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQQLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          711 a          680 c          733 g          785 t
ORIGIN      
        1 attattcaac tttatggtga ggattacaca atgagcagtc caaatattct cttaacccgt
       61 attgataacc gtctggttca cggtcaggtt ggcgtgacct ggacatccac catcggtgcg
      121 aatctgctgg tagtcgtgga tgatgttgtc gctaacgatg atattcaaca gaaattaatg
      181 ggtattaccg cagaaaccta cggctttggc attcgtttct ttactatcga aaaaaccatt
      241 aacgtcatcg gcaaagctgc accgcatcag aagatcttcc tgatttgccg tacgccacaa
      301 acggtacgta aattggtaga aggtggcatt gacctgaaag atatcaacgt cggcaatatg
      361 catttctcgg aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc
      421 gcggacttac gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc
      481 gatcaaaaag aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa
      541 taaaaatgca tgaaataacc ctacttcagg gattatccct ggcggcgtta gttttcgttc
      601 tggggattga tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc
      661 taactggcgc tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt
      721 tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg
      781 gtctgatgac caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc
      841 ttggcctgcc gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct
      901 cattatttat gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc
      961 ggcttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggcgatt gctttcctct
     1021 gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga
     1081 cccacggctt tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc
     1141 gcgtaatgtt caaagcgcaa tatatccctt acctgatcgc tggcttcctg tttgtttgct
     1201 acatccaggt cagcaacctg ttgccggttg ccgtactggg tgcaggcttt gcggtgtatg
     1261 agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg
     1321 aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc
     1381 tttcgttcgt cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt
     1441 acctgggcga tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc
     1501 gcggcgatga aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg
     1561 atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacactat taaaggcctc
     1621 aaagtggcac tgtttggccc aattgccggg attggcgatg cgattttctg gtttactttg
     1681 ttgccgatta tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg
     1741 attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc
     1801 ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct
     1861 cgctcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat
     1921 attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat
     1981 ttcttcgata aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac
     2041 ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct
     2101 attgtttgtt ccgcattcgg cattttgtaa atggaacgag gcactgcgtc tggcggtgcc
     2161 tctttactta aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg
     2221 ttaagtgaac gtgccagcga atatttattg gccgtgatcc gtagcaaacc ggatgccgtg
     2281 atttgcctgg cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc
     2341 caccagcagc aggttgatgt cagccagctc accttcgtga agctcgacga atgggtggat
     2401 ctgccattaa cgatgcctgg tacctgcgaa actttcctgc aacagcatat cgtgcagcag
     2461 ctggggctac gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag
     2521 tgcgaacggg taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctagga
     2581 ttggggaaaa acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc
     2641 catatcagtc aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc
     2701 cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg
     2761 ttattggtga ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc
     2821 tccaccgcta tcccggcttc gtttttatgg ctgcacagta actttatttg tttaattaat
     2881 acctaaccag atgaaaattt gctcaaaat
//
LOCUS       DAZFWP020000299         2834 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFWP020000299 DAZFWP020000000
VERSION     DAZFWP020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.301"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            235..960
                     /locus_tag="ACHK0Z_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     CDS             235..960
                     /locus_tag="ACHK0Z_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4504892.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            918..1595
                     /gene="bglJ"
                     /locus_tag="ACHK0Z_RS25245"
                     /old_locus_tag="pgaptmp_005049"
     CDS             918..1595
                     /gene="bglJ"
                     /locus_tag="ACHK0Z_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU4504893.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGSPYKLHTYTSQ
                     ESFQDAMSRISFTAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1633..2421)
                     /gene="fhuF"
                     /locus_tag="ACHK0Z_RS25250"
                     /old_locus_tag="pgaptmp_005050"
     CDS             complement(1633..2421)
                     /gene="fhuF"
                     /locus_tag="ACHK0Z_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU4504894.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2562..2798
                     /locus_tag="ACHK0Z_RS25255"
                     /old_locus_tag="pgaptmp_005051"
     CDS             2562..2798
                     /locus_tag="ACHK0Z_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU4504895.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          757 a          601 c          706 g          770 t
ORIGIN      
        1 ctaacaactt aatgtagata ttttaaatgt ctccaggcta tttcactagg ttatcatttg
       61 atgctaacct tctgacacat gcagtggagt tgttgtgcag caggagtatg ctgatatgaa
      121 agtgaatgct aaggataatt tattcgctta atctattaat ttgctggaaa catttaagga
      181 atgaatatcc tgaataaacc atcataattt ttagacagtg gatgtggagg aaatatgttg
      241 ccaggatgct gcaaaaatgg aattgttatc agtaaaatac ctgttatgca agcagggcta
      301 aaagaggtca tgaggactca cttccctgaa tatgaaataa tatccagcgc ctctgcggag
      361 gaccttacct tattacaatt acgtcgttcc ggattagtca ttgctgattt agccggtgaa
      421 agtgaagatc cacgttctgt ttgtgaacat tattattctt taatctcaca atatcgggaa
      481 attcactggg ttttcatggt gtcacgctcc tggtattccc aggcagtaga actgctcatg
      541 tgccctacgg cgacgttatt gtctgatgtt gaacccattg agaatctggt caagaccgta
      601 cgttccggca atacgcacgc agagcgtatc agcgccatgc tgacctctcc ggcaatgact
      661 gaaactcatg attttagcta tcgctccgtc attctcactc tttcagagcg caaggtactg
      721 cggctattag gtaaaggatg gggcatcaac cagatagctt cattgcttaa gaaaagtaat
      781 aaaactatca gcgcccaaaa aaacagtgcg atgcgtcgac tggcaattca cagcaacgct
      841 gaaatgtatg catggataaa tagcgcgcag ggtgcaagag aacttaactt gccttctgtt
      901 tatggagatg ccgcagaatg gaacacagcc gaattaagaa gagaaatgtc gcactcatag
      961 aaaaatgcgt catgagtagt atcggtattg agagtttatt cagaaagttt gcgggtagcc
     1021 cttataagct ccatacctat accagtcagg agtcatttca ggatgccatg tcgcggatct
     1081 cgtttacggc ggtcattttt tctttttctg ccatgagaag tgagcgcaga gagggattat
     1141 cttgcctgac tgaactggcg attaagtttc cgcgtacccg gcgtttagtt attgcggatg
     1201 atgatattga agctcggctg attggttcat tgtcgccatc accgctggac ggtgtattaa
     1261 gtaaagcgtc aacgctggag atttttcatc aggaactctt tttgtcatta aatggtgtac
     1321 gtcaggcgac cgaccgactg aacaatcagt ggtacattaa ccaaagccgg acgctaagcc
     1381 cgacggagag agaaatattg cgctttatgt cgcgtggcta ctcaatgaca caaattgccg
     1441 agcagcttaa acgtaatatc aaaacgatcc gtgcacataa atttaatgtg atgtcgaaac
     1501 tgggcgtcag ttctgatgca gggttgttgg aggccgcaga tattctgtta tgtatgcggc
     1561 attgcgaaac aagtaatgtg ttgcatccct attaatccgc atgatgccgg gtttacttcc
     1621 ccggcagtgc tttcatttca gcgtacaatc gccacattgc tgcacatccg gtaagcgata
     1681 acgctggcag caagtgcggc gcaccagcag gccatcgcgc agtaccacgg tacgccagag
     1741 tggattatct tcaccgttcg tgagcgtttt ctcaaaaaag agggcatggc gcagcgattc
     1801 aaccgtcgcc tcgccgagca gttgcttcat ctcagtaaga taccagttga tcaaataacc
     1861 ggtattactc cagataagtt tgccgttgat ctctccggtc gcttctagtg cctgcacaac
     1921 cggaaccagc gcctggctga ttaacctttc cattcgctgc tgcggcgaat gtggtgttgc
     1981 gtttttatct tcacacacat cgacccagaa acaggcggca cggccggttt cgtgaaactc
     2041 agcatggaaa tgttccggcg acacatctaa tgccttttcc tgcgtcagta gcgccagcat
     2101 taatggtggc accatcaggc cgatatacca ttgtgcccat agtgagatca gcggtttgtt
     2161 ctcgcggatc atcgttggtt ggttgcgata gatatgatcg gaatagaccg cgagtagaga
     2221 acttagcgca ttcggtgatg accattgcgc cagcgtcatg gcgttaagcg gagcgggttc
     2281 atccaggcgg ataaactcga gcaaatgttc acgatgtttg gcgatcgtcg cacgtacggc
     2341 ttgtgcaagc ccagcatcct gcggctggag atgcgttcgc cagatgatat cttcatagag
     2401 cggtgcggaa cgataggcca taatcgggat agtaatctaa atgataatga ttgctaatcc
     2461 tagcgatagg tttacccgat agcaagggat ttctttggct tgcaaatgat aaaaattatc
     2521 atatgatatt ggttatcatt atcaatgaaa gagatgaaac catgttgcaa cgtatgctgg
     2581 gcagtggctg gggagtgttg ctgccaggat tgctgattgc agggctgatg tatgcggatt
     2641 tatcgccgga tcagtggcgg attgtcattc tgatgggatt agtattgacg ccggtgatgc
     2701 tgtatcacaa acagttacgg cattacgttt tgctaccatc gtgcctggcg cttattgctg
     2761 gcatcatgct gatgataatg aatttgaatc agggatgaaa aatcaaggaa gaaacaagaa
     2821 aggaagtaaa gata
//
LOCUS       DAZFWP020000300         2793 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFWP020000300 DAZFWP020000000
VERSION     DAZFWP020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.302"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            468..683
                     /locus_tag="ACHK0Z_RS25260"
                     /old_locus_tag="pgaptmp_005052"
     CDS             468..683
                     /locus_tag="ACHK0Z_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4504896.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            683..1180
                     /gene="rrrD"
                     /locus_tag="ACHK0Z_RS25265"
                     /old_locus_tag="pgaptmp_005053"
     CDS             683..1180
                     /gene="rrrD"
                     /locus_tag="ACHK0Z_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4504897.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKSACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            1177..1614
                     /locus_tag="ACHK0Z_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             1177..1614
                     /locus_tag="ACHK0Z_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4504898.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDRITAQVNYLQEYIRTQCLK"
     gene            1764..2381
                     /locus_tag="ACHK0Z_RS25275"
                     /old_locus_tag="pgaptmp_005055"
     CDS             1764..2381
                     /locus_tag="ACHK0Z_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4504899.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAIMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(2569..2763)
                     /gene="ylcI"
                     /locus_tag="ACHK0Z_RS25280"
                     /old_locus_tag="pgaptmp_005056"
     CDS             complement(2569..2763)
                     /gene="ylcI"
                     /locus_tag="ACHK0Z_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4504900.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          742 a          615 c          723 g          713 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgactgac ctgaaaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgcct tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcaaaaggat agatgtactt
      241 cacatgttgc ttctatatgg cagtacattt tctgacagac agtgacggat gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg cgttggccgt cagtgggtga cactggcggc
      421 ttttttgttt ttctttactt tcattttctg tcggcggtga cggagacata catcagatgg
      481 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac
      541 tgcagctgct ggacaaagtc tctccgtccc agtgggtggc aataggtgtg ctgggaagtc
      601 tgctgtttgg cctgctgacg tatctgacaa atctttattt caagattaaa gaagataagc
      661 gtaaggctgc gagaggtgaa taatgtcgcc gtcattacgc aaggctgttg cagctgctat
      721 tggtggtggg gctgttgcca tagcgtctgt gctcatcact ggtccaggtg gtaacgatgg
      781 tctggaaggt gtcagctaca taccatacaa agatatcgtt ggcgtatgga ctgtatgtca
      841 cggacacacc ggaaaagaca tcatgcccgg taaaacgtat accgaagcag aatgcaaagc
      901 cctcctgaat aaagaccttg ccacggtcgc cagacaaatt aacccgtaca tcaacgtcga
      961 tataccggaa acaacgcgcg gcgctcttta ctcgttcgtt tacaacgtgg gcgctggcaa
     1021 tttcagaaca tcgacgcttc ttcgcaaaat aaaccagggc gatatcaaaa gcgcatgtga
     1081 tcagctacgg cgctggacat acgctggcgg taagcaatgg aaagggctga tgactcgccg
     1141 tgagattgag cgtgaagtct gtttgtgggg gcaacaatga gcagagtaac cgcgattatc
     1201 tacgttctgg tcatctgcct catcgtctgc ctttcatggg ctgttaatca ttaccgtgat
     1261 aacgccatcg cctacaaaga gcagcgcgat aaagccacat ccatcatcgc tgatatgcag
     1321 aagcggcaac gtgatgtagc agaacttgac gccagataca caaaggagct tgctgatgct
     1381 aatgcgacta tcgaaagtct ccgtgctgat gtttctgctg ggcgtaagcg cctgcaagtc
     1441 tccgccacct gtgcaaagtc aacgaccgga gccagcagca tgggcgatgg agaaagccca
     1501 ggacttacag cagatgctga actcaattat taccgtctcc gaggtggaat cgacaggata
     1561 accgcgcagg ttaactacct gcaggaatac atcaggacgc agtgcttaaa ataattttaa
     1621 tttcactgaa atttaacaag tgactttcag gaaaatgcct cgcagatgcg gggcattttt
     1681 gtaccggtat ttcaccgcgc accgcagcgc acaataaaca ccgaacctga ccctttggaa
     1741 tgggcctttg aggataccag ttagtgctgg cgagcctcgg tgggctggtt tcctgtgcgg
     1801 caaaggttca tttcaaagaa gcaggcaacg ccatgaatga attaattgcg aatcatgact
     1861 tcgactttcg ccagttagtt accgcagcag aaggtcaacc ggtaactgac accttccaga
     1921 ttgccagggc atttggtaaa cgccatcagc atgtgattag ggctattaaa tgtttgagat
     1981 gttctgagga attctcgaca acccattttt gggccgtcga gaaaatcaat gacttaggga
     2041 tttttgacaa gaaacagatt tactaccgca tggactttag tggcttcgtt atgctggtta
     2101 tgggatttaa cggggcaaaa gccgatgctg ttaaagaagc ctatatcaat gcgtttaact
     2161 ggatgtcagc agaactccgt aagtacagcg aaagttatga agcagaacgc aacgccataa
     2221 tgctggagta tatgaaagag aaggatgtcg ccagcatgtc aggccgtctg ctcaatcgct
     2281 gggggagaac gaaaaaacct caattgcttg caaagctgga acgtctggag agacagggac
     2341 agtttttatt accgggattc gataaaggta ttcaagcctg acacattatg cgctgtatcg
     2401 tcgccgtatt cccgcattaa ccatgaccgt agcccgacgg ggaattcctt ctgcgtgagt
     2461 gtgcgggaat aatcaaaaac gatgcacacc gggttttact gtgctgacag acgcagggtt
     2521 accctcatag tcgcttttcc ggtgcgatgg tggaagaaac cgggatgttc atccatcatc
     2581 actttggatt gatgtatatg ctctcttttc tgacgttagt ctccgacggc aggcttcaat
     2641 gacccaggct gagaaattcc cggacccttt ttgctcaaga gcgatgttaa tttgttcaat
     2701 catctggttt ggaaatcgga tgttgcgggt tgttgttctg cgggttctgt tcttcgttga
     2761 catgaggttg ccccgtattc agtgtcgctg att
//
LOCUS       DAZFWP020000301         2778 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFWP020000301 DAZFWP020000000
VERSION     DAZFWP020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.303"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            77..2356
                     /gene="ydeP"
                     /locus_tag="ACHK0Z_RS25285"
                     /old_locus_tag="pgaptmp_005057"
     CDS             77..2356
                     /gene="ydeP"
                     /locus_tag="ACHK0Z_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGU4504901.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2604..>2778
                     /gene="safA"
                     /locus_tag="ACHK0Z_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     CDS             2604..>2778
                     /gene="safA"
                     /locus_tag="ACHK0Z_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HGU4504902.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHF"
BASE COUNT          741 a          654 c          678 g          705 t
ORIGIN      
        1 cgataatccc tttgtatgta ctgtgcatca tcgctattac aaatcctaat aattcatttc
       61 cacacaggat aagtagatga agaaaaaaat tgaatcctac cagggtgctg caggtggttg
      121 gggtgctgtt aaatccgtag cgaatgcagt acgtaagcag atggatatac gccaggatgt
      181 tattgccatg tttgacatga ataagccaga gggctttgac tgtccgggtt gtgcatggcc
      241 agatcctaag cacagtgcgt cattcgacat ttgtgaaaac ggcgcaaaag caatcgcctg
      301 ggaagtcacg gataagcagg taaatgcctc tttctttgct gagaatacgg ttcaatcatt
      361 acttacctgg ggagatcacg agcttgaggc tgcgggacgt ctcactcagc ctttgaaata
      421 tgatgccgtc agcgactgtt acaagccatt aagctggcaa caagctttcg acgaaattgg
      481 cgcacgcctt caaagctata gtgatcccaa tcaggttgaa ttctatactt cgggccgcac
      541 ttccaatgaa gctgcctttc tttatcagct ttttgcccgt gaatacggga gcaataactt
      601 tcccgactgc tccaacatgt gccatgaacc gaccagtgtg ggtttggcag cgagtatcgg
      661 cgtgggtaaa gggactgtgt tgctggaaga ctttgagaag tgcgatttag tcatttgcat
      721 tgggcataac cctggtacaa accaccctcg catgctgact tcgttgcgcg ctttagtgaa
      781 acggggagcg aaaatgatcg ccatcaatcc tctacaggaa cgtggcctgg agcgatttac
      841 cgcaccgcaa aacccgtttg aaatgctgac gaactctgag actcagttgg ccagtgccta
      901 ctacaacgtg cgcattggtg gcgatatggc gttgctcaag gggatgatgc gcctgttaat
      961 tgagcgcgat gatgctgcaa gtgccgcagg tcggccctca ttgcttgatg acgaatttat
     1021 tcaaacgcat accgtcggct ttgacgagct acgccgtgac gttctcaatt ccgagtggaa
     1081 agatatcgaa cgtatttctg gactaagtca gacacaaatc gccgaactgg ctgacgcata
     1141 tgccgctgcc gaacgcacca ttatctgtta cggaatgggg atcactcagc acgaacatgg
     1201 tacccagaat gtacagcaac tggtcaatct gctgttgatg aaaggtaaca ttggcaagcc
     1261 tggtgcgggt atctgcccac tacgtggcca ctctaatgta cagggcgacc gaaccgtcgg
     1321 tatcaccgag aaaccgtctg cagagtttct ggctcgcctg ggtgagcgct atggcttcac
     1381 accacctcaa gcacctggac atgctgcaat tgccagcatg caagcaatat gtacagggca
     1441 ggctcgagca ttgatctgca tggggggcaa ctttgcactg gcaatgccag atcgggaagc
     1501 gagcgctgta ccgttaacgc aattagatct ggcggtacac gtagccacta agcttaaccg
     1561 ctctcatctg ctgaccgcac ggcatagcta tattctgccg gttctgggac gtagcgagat
     1621 tgacatgcaa aaaagcggtg cgcaggcggt aaccgttgag gattcaatgt cgatgattca
     1681 tgcctcgcgt ggcgtgttaa aacccgccgg tgtaatgctg aaatcagagt gtgcagtggt
     1741 cgcgggaatc gcgcaggcag cactacccca gagcgtggta gcctgggagt atctggtgga
     1801 agattatgat cgcattcgca atgacattga agctgtgctg ccagagttcg ccgactataa
     1861 ccaacgcatc cgtcatcccg gtggttttca cctgataaat gcagctgctg aaaggcgctg
     1921 gatgacgccg tcaggtaagg ctaatttcat taccagcaaa gggctgttag aagatccctc
     1981 ttcagcgttt aacagtaagc tggtcatggc gacagtacgc agccacgatc agtacaacac
     2041 gacgatttat ggtatggatg atcgctatcg aggggtattc ggtcaacgag atgtggtctt
     2101 tatgagtgct aaacaagcta aaatttgccg tgtaaaaaac ggcgaaagag ttaatcttat
     2161 tgcgctcacg ccagacggta aacgtagctc acgccgcatg gatagattaa aagtggtcat
     2221 ttaccctatg gctgaccgct cactggtgac ctattttcca gaatcgaatc acatgctaac
     2281 actcgataac cacgatccat taagtggcat tcctggctat aaaagtattc cggttgaatt
     2341 agaaccatca aattaatgcc tcttctcatt tcttctgctg tcatccgcac agcagaagaa
     2401 tccctcattg actattatcg cgcaatttgc tcacatggat taaattaaac tacatactat
     2461 aagatataaa cctctgccta cagctgtaag aaactccgct cagtactgaa gtgccagtcc
     2521 tatttcctct tttctccagc ctgttatatt aagcatactg attaacgatt tttaacgtta
     2581 tccgctaaat aaacatattt gaaatgcatg cgaccacagt gaaaaacaaa atcacgcaaa
     2641 gagacaacta taaagaaatc atgtctgtaa ttgtggttgt cttattactg acccttacgt
     2701 tgatagccat tttttcggca attgatcagc tgggtatttc agaaatgggt cgcattgcaa
     2761 gagatcttac acatttca
//
LOCUS       DAZFWP020000302         2767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFWP020000302 DAZFWP020000000
VERSION     DAZFWP020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.304"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(430..603)
                     /locus_tag="ACHK0Z_RS25295"
                     /old_locus_tag="pgaptmp_005059"
     CDS             complement(430..603)
                     /locus_tag="ACHK0Z_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504903.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            781..984
                     /locus_tag="ACHK0Z_RS25300"
                     /old_locus_tag="pgaptmp_005060"
     CDS             781..984
                     /locus_tag="ACHK0Z_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HGU4504904.1"
                     /translation="MVLSDCYSLANDQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(916..1149)
                     /locus_tag="ACHK0Z_RS25305"
                     /old_locus_tag="pgaptmp_005061"
     CDS             complement(916..1149)
                     /locus_tag="ACHK0Z_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504905.1"
                     /translation="MSQQPVRLRVSGGIPQVIKCRTVTEVNGGGIFQREEQRMCLTCP
                     CSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1159..2118
                     /locus_tag="ACHK0Z_RS25310"
                     /old_locus_tag="pgaptmp_005062"
     CDS             1159..2118
                     /locus_tag="ACHK0Z_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU4504906.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGW
                     FRHQDMAEGYLVMKSLDQEICSRDRSKMGDNVWCCITAQASHS"
     gene            2283..2767
                     /locus_tag="ACHK0Z_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /pseudo
     CDS             2283..2767
                     /locus_tag="ACHK0Z_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          731 a          687 c          651 g          698 t
ORIGIN      
        1 acacactgac aaaaaaccaa gcatggtaat aaaccgaact ccccacaaac ttctgttgcc
       61 tcagatactc cccccctttc ctcaggcaca aatgttgttc cataacgaac cagccctgtt
      121 cctttaaact ctgcatcaat ggcataatgt atactcacca gactgagttt gctcctgcat
      181 cattatttcg atacgttaat gcattgttca ttgcatccgg cattccccat cgaatattta
      241 ctttgttata tcagtcagcc cataaccagg taaattttta aggcttactg actgaatatt
      301 caaaatgtgt tacatgttat caaaccttta aagaataacc tgctcttctt cattggccgg
      361 atttagctca atcttgtcag caaaactaat ataaagccgt tcataaagag aaggatgtag
      421 cttcgttatt tatcttttac atgtaacagt gccaggggat attccgcaca tctctgtggc
      481 tatcatcccc accacgtctc cagtggcagc acctgcggca ctggcacggg cgttatttcc
      541 ctgtaccacc gccagtgcag cattcaccac ggcatgggct gccacatttg cagtcttatt
      601 caccacacct gtaacctgtc tcttatacac aaatccccac ccggggattt gtgatgtctg
      661 tcagtctgct ttagggggac tctttccatc cggtagtttc cttcctcccc ggcagcgatt
      721 ttttgaactt tttcttcacc cgttgatctt ctcttatatt tcattttctt cgggtctgtt
      781 atggtacttt ctgattgtta ttcactggct aacgatcagt ctggacatgc ccgtctcggt
      841 gatccgcgtc gtacacgacg ccttgtgtca ctgacctctt ctctcgctca gcatgcaggt
      901 ctttctattg tgaaatcatc ccacttcact gctcaggttg aaggtgcgta ccgcctgata
      961 cgtaacccgt cggtgtcccc gtaagccatt gccgaagccg gatttactgc cactgcaagg
     1021 gcatgtgagg cacatccgct gctcctcgcg ctggaagata ccaccaccat taacttcggt
     1081 cacagtacgg catttgatga cctggggaat accaccacta acccgaagac gcacgggctg
     1141 ctggctcact ctgtcctgat gtacgccccc gacagcgccc tgccagtcga tcttatcgaa
     1201 cagcagtgct ggagtcgtgc cacggacacc tacggtgtta agcaccagcg aaaagaacgt
     1261 ccctgtgaag aaaaagaaag ttaccgctgg cagcaggctt cggagcgcat ggccgaacgg
     1321 ctgggggaga tacagaaaca ggtaatcact gtctgtgacc gggaagcgga tatctggcat
     1381 tacctgcatt ataaagtcag ccgcgggaag ccacacgctg aatgtgatgc aaaaaggcgg
     1441 gcgggcagcc cgccaggccc ggatgctcat cagttacagc gaagtcagca taaaaaatcc
     1501 cgacaacagc ggccaggcgc tctcgctcac gtatgtctgt tgcggggagc aggagaggac
     1561 ggagcctgct ggtatccgct gacatcagaa aaagtggcga gtgccgccga tgcgcgacgt
     1621 atcgtcagcc attacgagca acgctggctg atagaggaat accacaaagc gtggaaaagt
     1681 ggtggtacat gtgtggaatc gctgcgaatg cagacccggg ataaccttga gcgcatggtg
     1741 gttatcgcgg tgcgggtgct gggcctgcgg cagggaggta tcagtgaaga aacacaaaat
     1801 gacagctgtg agaaaatact gacgccgaca gagtggaaac tactgtgggt taagctggaa
     1861 ggaaagcagc ttccggcgca aacaccgacc ctgaaatggg cctgtttgaa actggctaaa
     1921 ctggggcgat ggcatgacag caaacgcaca agcagccccg gttgggtagt tatgtgggat
     1981 ggctggttca gacatcagga tatggctgag ggttacctgg tgatgaagtc tcttgatcag
     2041 gagatctgct caagagacag gtcaaagatg ggggacaacg tctggtgctg tataacagca
     2101 caggcctcac actcttgacg cccgtggaat atattagaga acaaaaattt caatatgtgg
     2161 tggacgcata cagaggattg acagcattcc catactaatt gttaagattc aaattgaagt
     2221 atatctatct gtcattttgc aaatgatatg cggtagcttt acctaactat catgaacatt
     2281 tcgaaaaatc atccacaaag ccccgaagaa taaagcacta tcgcccgata aagacctgta
     2341 agctcagtgg catggatact attgagctca gaatggcgag ctgtaccgtt atttcattac
     2401 catgctcgac gaacacagca actactctct ggccgtacta tcgcttaaaa gtgatatcat
     2461 caggcatttc ttctgctgtg tagcccagtt gttccttgtt ggtatcaacc agatcatcac
     2521 tgatgatggg caaagagttc tcgggaaaat tcgacaaaac gctacaggaa gccaccgtcg
     2581 ggttcgtatg gacttatctc tatacgccga aaattaaatg ccataagtga gcattttaac
     2641 cgaacactga gggaacaatt tattgagttt aatgaagaat tgctctttga atagctgaat
     2701 atgttcaatc tgaaactggc tgagtatctg gtactgtaca acagtaaaat tcttcataaa
     2761 tcgttta
//
LOCUS       DAZFWP020000303         2766 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFWP020000303 DAZFWP020000000
VERSION     DAZFWP020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.305"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..98)
                     /locus_tag="ACHK0Z_RS25320"
                     /old_locus_tag="pgaptmp_005064"
     CDS             complement(<1..98)
                     /locus_tag="ACHK0Z_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504907.1"
                     /translation="MSSKNRTRRTTIRNIRFPNHMIEQINIALEHKG"
     gene            140..505
                     /locus_tag="ACHK0Z_RS25325"
                     /old_locus_tag="pgaptmp_005065"
     CDS             140..505
                     /locus_tag="ACHK0Z_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4504908.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELEHRIALQFGGGNEETNLWTLCTECHRQKSAREAASGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            795..1358
                     /locus_tag="ACHK0Z_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             795..1358
                     /locus_tag="ACHK0Z_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4504909.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNLEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1355..>2766
                     /locus_tag="ACHK0Z_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             1355..>2766
                     /locus_tag="ACHK0Z_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4504910.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGANLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGW"
BASE COUNT          613 a          661 c          882 g          610 t
ORIGIN      
        1 cctttatgtt caagggcgat attgatctgt tcaatcatgt gattggggaa acggatattg
       61 cggattgtgg ttctgcgggt ccggtttttc gatgacattt tctttcctct ggtgacaagc
      121 tatatggaga ggattttaca tggctgtgct tcgtacgtta ccgggcagaa tcaaaactct
      181 gaacacccgg cgggtgaatg tcctgaaggg tgaacagcgt cgggtcagtg gtagtgcccg
      241 ggtttccctc aagcgtcgta tctggctgag ggatgccgga cagtgctgtc tctgtgggcg
      301 tgtggttgac ctttgtgaca gtgaacttga gcaccgcatt gcacttcagt tcggtggtgg
      361 taatgaggag acgaatctct ggacgctctg taccgaatgc catcgacaaa agtctgctcg
      421 tgaagcggcg agtggtatgc cagacccgac gctgccggag gtgtccggag gtagtggcag
      481 agcggacgac atcatcggac tgtaacccga cccggggggg tatcatccgg cgtaaaaaaa
      541 gatcgctttg gacaccgcgc cccctctcac gcagagaaaa aattcccgtt tcagggcagt
      601 taacatgtta actggctgcc cgggcatttt tgcggttttt atctttatta ttcagtttgt
      661 tgtgcggaaa aaatgttaac aggctttttc agcaaatgtt aaccaggcag cagttaacat
      721 ttgcggcatg agacgccggg aaaaatgggc tgaaccatac ccggctgagt gcgttctgga
      781 cccgggagga ggctgtgctg acaacgcaaa aacgaaaatt tgcgctggcg ctcatgtccg
      841 ggaaaaacaa aacagcgtca gccattgccg ccggttattc ggcgaagacc gccagggtta
      901 aaggctcgca gctggcaaaa gatcctgagg tgcttgcgtt tatagcccgt aaacaatgcg
      961 agacggtgga ggtggatgag gttcctgttt accggcagaa aaaatcagag caggaggata
     1021 aaccccgtcg ccgtgaggcg gctgcaatac cacagccgga cgaaaacaat ctggagatgc
     1081 caccgtccgc ggtgatgtct cctggtattg aatatatgga ggatggtctt cccgatccgg
     1141 tgaaagccat ggggcggatc ctggtggaaa acctctgcat tgatccgaaa ctggcactgg
     1201 atgcggcctg gcgtctggcg cagttcacgc accataaaaa aggggatacc gggaaaaaat
     1261 cggcaaaagg tgatgccgcg aaaaaagcgg ctaaccgttt tgcggtgcca ccgccacccc
     1321 gactggtggt gaataatgat aatgagggca acggatgata cctgtatgga gcacagcctg
     1381 cccggactgg gcagagcgcc tgaaaaaggg gctgtcgatt attccggatc cgatttatcc
     1441 ggacgaggcc gcacatgccc tggcgatttt taaacaactg cggattgtgg atgcacctgg
     1501 tagccctacg ttcggggagt cctgtgcacc gtgggtgttt gacctggtgg cggccctgtt
     1561 tggctcctac gatgcgcaga ccggtgtacg ccatatcaag gaagttttta tccttatccc
     1621 caagaaaaac tcgaagtcca cgctggctgc ggggatcatg atgactgcac tgttactgaa
     1681 ctggcggcag gcggcgggtt acacgattct ggccccgact gtggaggtgg cggccaacgc
     1741 cttcaaccct gcccgggata tggtacgacg tgacgatgat ctggatgacc tctgccaggt
     1801 acagacccat atccggacca tcacccaccg ggtgacggac accaccctga aggtggtggc
     1861 agccgatccg aatacggtgt ccggtatcaa gtccgtgggt acgctgattg atgagttgtg
     1921 gctgtttggc aagcagtgca aggcggagga catgttacgt gaagccatag gcggccttgc
     1981 ctcccgcccg gaagggtttg tggtgtatac gaccacccag tcgaatgaac cgcccgccgg
     2041 ggtgttcaga cagaaactgc agtacgcccg ggatgtgcgc gacggcaaaa ttcatgatcc
     2101 gcactttctg ccggtgatat ttgaacaccc tcctgaaatg gtggaaagcg gggctaacct
     2161 gctgatggaa aacctcgcca tggtcaatcc gaatctcggc tattcagtgg atgaggcctt
     2221 tctgtaccgg gagtaccgta aagcccggga agccggtgaa gagacattcc gggggttcat
     2281 gtcaaaacac gccaatgtgg aaattggtct tgccctgcgc tctgaccgct gggcgggggc
     2341 tgatttctgg gaagagcagg gccgttgtat cagcctggac gatatcctgc gtcgtgctga
     2401 tgtggtgacg gtggggattg acggcggagg gctggatgat ctgctgggga tgtatgtgat
     2461 tgggcgtgac cgggagaccc gcgaatggct gggctggggc catgcctggg cgcatgaaac
     2521 cgcggtggtc cgacggaaga gcgaggcgtc ccggtttcag gatcttgttg cctgtggaga
     2581 tatgaccatt gtccggcgtg tcggggatga cacggcggaa gtggcggaat atgtgcgtcg
     2641 cattcatgag gctgagttac tggaccatat cggtattgac ccgtcagggg tggggcagat
     2701 tctggattca ctggcggaag ccgggatccc cgacggaatt gtggtgggga taagccaggg
     2761 ctggaa
//
LOCUS       DAZFWP020000304         2749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFWP020000304 DAZFWP020000000
VERSION     DAZFWP020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.306"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            309..497
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             309..497
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4504911.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            494..682
                     /locus_tag="ACHK0Z_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             494..682
                     /locus_tag="ACHK0Z_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4504912.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            777..>2749
                     /locus_tag="ACHK0Z_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             777..>2749
                     /locus_tag="ACHK0Z_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4504913.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDECRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESYPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NTMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGSVKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAP"
BASE COUNT          823 a          640 c          677 g          609 t
ORIGIN      
        1 gttgaggaca ccgggttgtc aggttgacca tacgcttaag tgacaacccc gctgcaacgc
       61 cctctgttat caattttctg gtgacgtttg gcggtatcag ttttactccg tggctgctct
      121 gccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca
      181 gttaaaacca aaaacagtgt tatgggtgga ttctctgtat ccggcgttaa ttgttaactg
      241 gttaacgtca cctggaggca ccaggcacca ggcaccgcat cacaaaattc attgttgagg
      301 acgcgataat ggaaacgtta ttaccaaacg ttaatacgtc tgaaggttgt tttgatattg
      361 gtgttctgct cagtaaccgg gagtttactg aagatgccat taatatgagg aaatatgagc
      421 cttatctgct caatgataat tccatacttt cccgaattgc tcttcttgaa cttggtattt
      481 tcggagaacg tcaataactt cagcatttgc actgatgatg acggtttttc ttataacggg
      541 tgaatcacag aatgtgatta ccggaattta tgccagtaaa gaatcctgcc tccaggcaag
      601 agacgagcaa aaaatttctg gtgaatgcct cccggtaaaa aaagtatcgc tgtacctgaa
      661 taacgaaaca ccggctggat aaccctccag ccatattaac accataccaa cggattaaaa
      721 atgccagcaa tggcagggat tcgttcaccc tgaaatctgt aatgaggtaa aacaaaatga
      781 gtaaagtctt tatttgcgcc gccattccgg acgaacaggc aataaaggaa gaaggtgcag
      841 tcgctgtagc cactgccatt gaagccggtg atgaatgtcg cgcccgcgca aaatttcact
      901 ggcaattcct ggaacattat ccggctgctc aggactgcgc ttataaattt attgtctgcg
      961 aggataaacc tggcataccc cgccctgccc tcgattcatg ggatgctgaa tatatgcagg
     1021 aaaaccgctg ggatgaggag tctgcttctt ttgtcccggt tgagactgaa tcctatccga
     1081 tgaacgtcac ttttgacaag ctggcccctg aagtacagaa cgctgtcatg gttaagttcg
     1141 acacatgtga aaacatcacc gttgatatgg tgattagcgc acaggaattg ttgcaggaag
     1201 acatggcaac attcgacgga catatcgttg aagcgttgat gaaaatgcca gaagttaaca
     1261 ccatgtatcc ggagcttaag ttgcacgcca ttgggtgggt taagcataaa tgtattcctg
     1321 gtgctaaatg gcccgaaatt caggcagaga tgcgcatctg gaaaaaacgt cgcgaaggtg
     1381 aacgcaagga aaccggaaaa tacacgtctg ttgttgatct cgcccgcgcc agagccaatc
     1441 aacagtacac tgaaaattca acaggaaaaa tcagcccggt cattgctgcc attcatcgcg
     1501 aatacaagca gacatggaaa acactggatg acgaactggc ctacgctctc tggcctggtg
     1561 atgtggatgc cggaaacatt gacggcagca tccatcgctg ggcaaaaaat gaagttatcg
     1621 acaacggccg cgaagactgg aagcgtatct cggcatcaat gcgcaaacag cctgatgccc
     1681 ttcgctacga ccgccagact atttttggcc ttgtccgtga acgtccgatc gacattcaca
     1741 aagatcccgt agcactgaac aaatatatct gcgaatacct gacgacaaag ggcgtgtttg
     1801 agaatgaaga aacagacctg ggcactgttg atgttctcca gtcatcagaa acacaaactg
     1861 atgcagtgga aactgaggta tctgatatcc caaaaaatga aaccgcgccg gaagctgaac
     1921 catctgtaga gcgtgagggg ccgttctact tcctcttcac cgacaaggat ggcgaaaaat
     1981 acggtcgcgc aaacaaactt tctggtctgg ataaggcgct gtctgctggg gctactgaaa
     2041 tcacgaaaga agaatatttc gcccgcaaaa acggtacata ctcaggttca caacaaaata
     2101 ctggtgcatc tgacacgacc gcacaaccag ggtcagtaaa agttaccgct gacgaagtaa
     2161 acaaaattat gcaggcagcc aatatcagcc agcctgacgc cgatgaactg cttgcagtat
     2221 cacgtggtga atttgttgaa gggattagcg acccgaatga tccgaaatgg gttaagggga
     2281 tccagacccg cgattctgtg aaccagaacc agcaagaaac ggaacagaac gaccagaaag
     2341 cggaacaaaa cagcccaaat acgcaacaaa acgagccaga aacgaaacaa cctgaaccag
     2401 tagtgcaaca ggaaccggaa aagatctgca ccgcctgcgg tcagagcggt ggcggcaact
     2461 gccctgattg tggtgcggtg atgggtgacg caacatacca ggaaatattc gatggagaga
     2521 atcagcctga agttcaggaa aatgatccgg aggaaatgga aggcactgcg catcagcaca
     2581 aggagaacac tggcggcaat cagcatcatg ccagcgatag tgaaactggc gaggcgtcag
     2641 atcccttaat taaggcgaac ggtcatcata atctcacatc caccagcaga gcggggattc
     2701 atctgatgat cgaccttgaa accatgggaa aaaatcccga tgccccgat
//
LOCUS       DAZFWP020000305         2720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFWP020000305 DAZFWP020000000
VERSION     DAZFWP020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.307"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..264)
                     /locus_tag="ACHK0Z_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             complement(<1..264)
                     /locus_tag="ACHK0Z_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGU4504914.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRYADYVIIATPTDYDPK"
     gene            complement(512..1918)
                     /gene="gndA"
                     /locus_tag="ACHK0Z_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             complement(512..1918)
                     /gene="gndA"
                     /locus_tag="ACHK0Z_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU4504915.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(2024..>2720)
                     /gene="galE"
                     /locus_tag="ACHK0Z_RS25365"
                     /old_locus_tag="pgaptmp_005073"
     CDS             complement(2024..>2720)
                     /gene="galE"
                     /locus_tag="ACHK0Z_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4504916.1"
                     /translation="ISNNIKNLIFSSSATVYGTPEKIPVKETFPVGGTTNPYGTSKLM
                     VENILHDVSLAYPFFRTTILRYFNPVGAHPSGKIGEDPNGIPNNLMPYICSVASGKYK
                     QLSIYGNDYETKDGTGVRDFIHVMDLANGHIAALKHRDEGSNYKVYNLGTGHGYSVLE
                     LLAAFQRITSVNIPYVLVERRAGDIAECWSDPSKAYLELGWKACLGLDEMVRDAWNWQ
                     QSNPDGYKFSEKN"
BASE COUNT          766 a          616 c          583 g          755 t
ORIGIN      
        1 tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatacc ggtaggcttc
       61 atttttatct aatgtagcat taaagtgtat tttatctgat tgcaaaaact gctgaatttc
      121 cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg gtaaaatatc
      181 taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc ctacatagcc
      241 ggtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact gtgaatcatg
      301 atgtttttag catcctgata agagctaaaa gttttaacgc tacgttgtca gatggtcaac
      361 gcaacgaaaa acaattcaga ttttctttat aagaatagct gatatttatt aagttaatat
      421 taagcagtga aatttagtct ataagataat tggcaaaaaa aagcccggcg caatatacgc
      481 caggcctcaa tttattgtta gttaaataag attaatccag ccattcagta tggaacacac
      541 cttctttatc aatgcgctta taagtatgtg caccgaaata gtcacgctgt gcctgtatca
      601 ggttcgcagg cagaacagcg gcacggtagc tgtcataata ggcaaccgca gcggcgaagg
      661 tgggaaccgg gataccgttc tgtactgcat aagcaacaac atcacgcagc gcctgctggt
      721 agtcatcggc aatttgcttg aagtacgggg ccagcagcag gttagcgatc tgcggatttt
      781 cggcatatgc atcggtgatt ttctgcagga actgcgcacg gatgatgcag ccagcacgga
      841 aaatcttcgc gatttcgccg tagttcagat cccagttgta ctcttcagac gcagcacgca
      901 gctgagagaa gccctgagcg taagaaacga ttttgcccag atacagcgca cgacgaactt
      961 tttcgatgaa ctcagccttg tcgccagctg gctgcgcttg cggaccagag agaactttag
     1021 atgcggcaac gcgctgatct ttcagagaag agatataacg tgcaaacaca gactcggtaa
     1081 tcagcgacag cggttctccg agatccagcg cgctctggct ggtccattta ccggtacctt
     1141 tgttagccgc ttcatccagg atcacatcaa ccaggtagtt accgtcttca tcttttttag
     1201 tgaagatgtc tttggtgatg tcgatcaggt agctgctcag ttcaccgtta ttccactcgg
     1261 taaaggtctg tgccagttct tcgttagaga gattcaggcc gcctttaagc agagaatagg
     1321 cttcagcaat cagctgcata tcaccgtatt caataccgtt atgaaccatc ttcacatagt
     1381 gacctgcacc atcggcacca atataggtaa cgcatggctc cccatcttca gctactgcgg
     1441 cgattttggt caggattggt gcaaccagtt cataggcttc tttctgccca ccaggcatga
     1501 tggaaggtcc tttcagcgca ccttcttcac caccggaaac accggtaccg ataaagttaa
     1561 aaccttcggc agaaagctca cggttacgac gaatggtgtc ctggaagaag gtgttaccac
     1621 catcaatgat gatgtcacct ttatcgaggt atggcttgag ggaatcaata gcagcatccg
     1681 tgcctgcgcc tgctttcacc attaacagga tgcgacgagg cgtttccaaa gattcaacaa
     1741 actctttcac cgtatagtaa ggaaccagtt tcttgcctgg attttcggca ataacttctt
     1801 ccgtcttctc acgggagcgg ttgaaaatag agacggtata accacggctt tcgatgttga
     1861 gcgctaggtt gcgccccatc actgccatac cgactacgcc gatctgttgc tttgacattg
     1921 tttactcctg tcaggggtta ctgcgaactt taacgcaggt ctatatgaag cttacggtaa
     1981 ctcaggttag aaaagtatta tatttttact gacgctaagc aattcagttt ttctcagaaa
     2041 atttataacc atcaggattg ctttgttgcc aattccaggc atcgcgaacc atctcatcta
     2101 atcccaaaca ggctttccat ccaagttcaa ggtatgcttt agacgggtcg gaccaacatt
     2161 cagcaatgtc gcctgctcgg cgctcaacca acacataagg tatattaaca gaggttatcc
     2221 gttgaaaagc agccaaaagt tcaagaaccg aataaccatg ccctgtgcct aaattgtaaa
     2281 ccttataatt gctaccttca tctcggtgct tcaaggcagc tatatgaccg tttgcgagat
     2341 ccataacatg aataaaatct ctaacgccag taccatcttt agtttcataa tcattaccat
     2401 agatggataa ttgcttatat ttaccacttg caactgaaca tatataaggc atcaaattat
     2461 ttggaatacc gttagggtct tctccaattt ttccggaagg atgtgcgcca acaggattaa
     2521 aataacgcaa aattgttgtt ctaaaaaaag gatatgccaa agaaacatca tgtaatatgt
     2581 tctcaaccat aagttttgaa gtcccatagg gattagtagt gccacctaca gggaatgttt
     2641 cttttacggg tatcttctct ggagtaccat atacagtggc tgaggagctg aatattagat
     2701 tcttgatatt attactaatc
//
LOCUS       DAZFWP020000306         2712 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFWP020000306 DAZFWP020000000
VERSION     DAZFWP020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.308"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            82..1056
                     /gene="cysB"
                     /locus_tag="ACHK0Z_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             82..1056
                     /gene="cysB"
                     /locus_tag="ACHK0Z_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU4504917.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1375..1515
                     /locus_tag="ACHK0Z_RS25375"
                     /old_locus_tag="pgaptmp_005075"
     CDS             1375..1515
                     /locus_tag="ACHK0Z_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU4504918.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1518..1685
                     /gene="yciX"
                     /locus_tag="ACHK0Z_RS25380"
                     /old_locus_tag="pgaptmp_005076"
     CDS             1518..1685
                     /gene="yciX"
                     /locus_tag="ACHK0Z_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU4504919.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1799..1894
                     /gene="ymiC"
                     /locus_tag="ACHK0Z_RS25385"
                     /old_locus_tag="pgaptmp_005077"
     CDS             1799..1894
                     /gene="ymiC"
                     /locus_tag="ACHK0Z_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU4504920.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2058..>2712
                     /locus_tag="ACHK0Z_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     CDS             2058..>2712
                     /locus_tag="ACHK0Z_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HGU4504921.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT          703 a          629 c          639 g          741 t
ORIGIN      
        1 tttattatta aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg
       61 cgtctaagtg gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc
      121 aatcataacc tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc
      181 agtaaacaag tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc
      241 aagcacctga cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc
      301 ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc
      361 tcgctgtata tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa
      421 ggctttattg agcgttatcc ccgcgtttct ttgcatatgc accagggctc gccgacacaa
      481 attgctgatg ccgtctctaa aggcaatgct gatttcgcta tcgccacaga agcgctgcat
      541 ctgtatgaag atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact
      601 ccggatcacc cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg
      661 ttggtgacat ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc
      721 gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg ctgacgtcat taaaacttac
      781 gtccggttag ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat
      841 cccgaccttg tgcgtgttga tgctcacgat atcttcagcc acagtacaac caaaattggt
      901 tttcgccgta gtactttctt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg
      961 catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc gctctaatga agaaattgag
     1021 gtcatgttta aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct
     1081 tttatgtagc gaaggtgccg gaatatattc tcttttgtta cttatttcta taccgtgcac
     1141 aatattttta tccctcatat ttatagggta aattacctgt aaagtagtgt ctaattaccc
     1201 gcctcgcaaa ctccagtttt caaatatcta tttcaggtca aaagattgaa ttattcactg
     1261 gagacgattc gtaaattcgc tggatttttg actaaagttt ctttaggatt tgtctcatcg
     1321 tatgattaat tacaccaatc gttcaacgtc ttgctgataa gtgatggcga ttatatgagg
     1381 ttagcaatgc cttctggaaa tcaggaaccc cgccgcgatc ctgaattaaa acgtaaagcc
     1441 tggctggcgg tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg
     1501 aaagtgtggg gataactatg gtcggtcagg agcaactgga gtcgtcacca ttatgccagc
     1561 atagtgacaa tgaaacagaa acgaaacggg aatgttccgt cgttattcca gacgactggc
     1621 aactaacatc gcagcagcaa gcctttatag aactgtttgc tgaagatgat cagccgaaac
     1681 aataattatc atcattctta ttacccattt ttaatgaatt aaagggcttt taatacaccg
     1741 cagcaataac agcttgagtt atctcaacac aaaataataa ccgttaaggg tgtagcctat
     1801 gatcaacaca aatatgaaat attggtcctg gatgggcgcg ttttctctgt cgatgctctt
     1861 ctgggccgaa ctcctctgga tcattactca ctgatccttg accccgctgc ggcggggttg
     1921 tcatttgctt tgccacaagg tttctcctct tttatcaatt tgggttgtta tcaaatcgtt
     1981 acgcgatgtt tgtgttatct ttaatattca ccctgaagag aatcagggct tcgcaaccct
     2041 gtcattaagg aggagctatg tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg
     2101 ccaaagataa aacttaccac tactacagcc tgccgcttgc tgctaaatca ctgggcgata
     2161 tcacccgtct gcccaaatca ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg
     2221 gtaactcggt taccgaagag gatatccacg cgctggcagg atggctgaaa aatgcccatg
     2281 ctgaccgtga aattgcctac cgcccggcaa gggtgctgat gcaggacttt accggcgtac
     2341 ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa acgcctcggc ggcgatactg
     2401 caaaggttaa cccgctctca ccggtcgacc tggtcattga ccactcggtg accgtcgatc
     2461 gttttggtga tgatgaggca tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg
     2521 aacgttatgt gttcctgaaa tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc
     2581 caggcacagg catttgccat caggttaacc tcgaatatct cggcaaagca gtgtggagtg
     2641 aattgcagga cggtgaatgg attgcttatc cggatacact cgttggtact gactcgcaca
     2701 ccactatgat ca
//
LOCUS       DAZFWP020000307         2686 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFWP020000307 DAZFWP020000000
VERSION     DAZFWP020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.309"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHK0Z_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHK0Z_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU4504922.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHK0Z_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHK0Z_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU4504923.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          667 a          663 c          644 g          712 t
ORIGIN      
        1 tgttgcgttt gtcatcagtc tcagcccgct tttcagcggg cttcattgtt tttaatgctt
       61 acagcactgc cacaatcgct tcgcacagcg gagccatgtt atctggtgtc atcccggcca
      121 catttacgcg accagaagca accgcatata cgccaaactc ttcgcgcaga cgcagcactt
      181 gttcttttgt caggccactg aaggagaaca tgccgttctg tttgatgata aagctgaagt
      241 cgcggtttgc gcctttttcc tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac
      301 gctggcgcat atcagtcagc tcttgttccc aaatcgcacg taacgcatcg ttgctcagga
      361 tggtggcaac aacagaagcg ccgtgtgctg gtgggttaga gtagttagcg cgaatcgccg
      421 ctttcatttg gctgaatgcg cgatcaacgg tttcactgtc ggcagcaacc agagtacaag
      481 cgccaacacg ctcgttgtac aggccaaagt ttttagagta ggaactggca acaatcagct
      541 ctttatgcat agccgcgaaa gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa
      601 aaccctggta agcgaagtca aacagcggta accagccttt ctcaacggag agttgtgcca
      661 gtgtctgcca ttgttccagc gtagggtcga taccggttgg gttatggcag cagccatgga
      721 acagcactac gtcgccagcc tgagcttcat tcaggctgtt aatcagtgca tcgaagtcaa
      781 gagtgtgatt ttccgcatca taataagcgt attcacgaac ttccagacct gcagagttaa
      841 agacgctctt atggttcggc cagcttgggt tgctcaccca cactcgctta acgctggtat
      901 tttttgccag gaaatcggca gctacgcgta gtgcgccagt gccccccgga gtctgtgccg
      961 tgcgagcacg tttgtcattg atcagggcgc tacctttacc aaacagcagt tcctgagtgc
     1021 agcgaccaaa ttcagggatg ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga
     1081 gcagatactg ttcagccttt ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt
     1141 tatagacacc aatcccgagg ttaattttgc cgggacgttc atcggcacga aacagatcgg
     1201 ccaggcccag aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc
     1261 attatggtta cagaagggaa gtccgctatc agggtaacgg gagatttaca aaattccaac
     1321 tattactgat gaaaacgcag gctgtttttg caagacgtga gattgctctg gaaggtataa
     1381 aaaaaacagg accaaagtcc tgttttttcg gcatttaaca aagaggtgtg ctattagaac
     1441 tggtaaacga tacccacagc aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa
     1501 tcgatctggt tgatgatgta gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt
     1561 gcgcccactt caaagtagtt caccagttca acatcaccga taccttctac gtctttcgct
     1621 ttagatttgg tgtaagcgat ggacggacgc agaccgaaat cgaactggta ttgcgcaact
     1681 aacagaacgt cttgcgtttt gttggcgaag ccgctggtgt ttgtaaattt attagtgatc
     1741 ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt cgcgtcgtac
     1801 ttcagaccag tagcccactg ttcagctttt ttaccgttgc caagaggttg agcttcttgc
     1861 aggttggtac ggtcagctgc accataagca ccaacgatac caaagccttc gtattcgtag
     1921 ctgatagaac cgccaacacc gtcgccgtta gaacggcgtg cagtgtcacg ctcgttttta
     1981 cccaggtact gaacagcgaa gttcaggcca tcaaccagac caaagaagtt ggagttacga
     2041 taggtagcaa cgccgccaac acgaccaacg aagaagtcat cgctgtatgc agtatcacca
     2101 ccaaattctg gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg
     2161 ccgtaatcga aagaaccaac gtcagcgtat ttaagacccg cgaatgccag acgcgttttg
     2221 ttaccagttt gagcgtcagc gccttcagag ttgttaccct ggaagttata ttcccactga
     2281 ccataaccgg tcagatcgga attgatttga gtttcccctt taaaaccaag acgggcatag
     2341 gtcatgtcgc cattgccacc gtaactgttt tcaccgttac ccttggaaaa ataatgcaga
     2401 ccaacagctt taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg
     2461 tttgcagtac ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc
     2521 attatttatt accctcatgg ttttttttat gacacctgcc actgccgtca ataagttctg
     2581 tcaataaaaa tttacggaac tattgatgag agtttggtgt ctttatgtgt ctgcaggcat
     2641 ctttccattc aaactaacgt ttcgctaccg tgaaagtgct acaaag
//
LOCUS       DAZFWP020000308         2652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFWP020000308 DAZFWP020000000
VERSION     DAZFWP020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.310"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            182..1411
                     /locus_tag="ACHK0Z_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             182..1411
                     /locus_tag="ACHK0Z_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4504924.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            1871..2503
                     /locus_tag="ACHK0Z_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             1871..2503
                     /locus_tag="ACHK0Z_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4504925.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          764 a          535 c          521 g          832 t
ORIGIN      
        1 gtgattttta tcacaaagga aatatgcctg agcagtagtc agagtcataa ctggcacgta
       61 aggtttgcaa ccactaaccc accaatagag gggtagatag ggcgttaatc tcccatactt
      121 aacctggttt atggtaaatt gccctccatt ttgtttaatt tgtagatgat acgttcagat
      181 aatgtctaat atttggtcta aagaagaaac tctgtggagt ttcgcgctct acggcacagc
      241 cgttggtgca ggcacgctct tccttcctat tcagttaggt tcggcagggg ctgtggtcct
      301 gtttattact gctctggtcg cctggccttt aacatattgg ccacataaag ccttatgcca
      361 gttcatcctc tcatcgaaaa catcagcagg tgaagggata acgggcgcag taacacacta
      421 ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct tttttgtcgt
      481 cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg caaagcatat
      541 ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa tcctgaatct
      601 catttttctg atgggacgcc atgcaaccat tcgggtaatg ggttttttgg tattcccatt
      661 gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac ctgatctatt
      721 aacgacccag gtagagttca atcagaatac ccttcaccag atatggatat cgattcccgt
      781 gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag acagacgtga
      841 aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg cttatctcat
      901 catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc caccttcgta
      961 tattgaagcg gctaaagaag aaggggtcac cattttatca gcgctttcta tgctgccgaa
     1021 cgccccagca tggctgtcaa tttccgggat tattgtcgca gtagttgcga tgtcgaaatc
     1081 attcctgggt acgtactttg gcgttattga aggtgccacg gaggtcgtca aaacaacact
     1141 acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccgc gcactatcaa ttatgttggt
     1201 atcgctgatc accttcattg tttgttgcat taacccgaac gcgatttcga tgatttacgc
     1261 gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgttgt caacgtatct
     1321 gatcccggcg cttaaaccct ggcgttccat cggaaatctg attacgctga tcgtgggtat
     1381 cctgtgcgta tcggtaatgt tctttagctg atatccattg tgaatggttc aaaattgcta
     1441 caattttgaa ccattccagt tttcataatg ttcattcgtt attattccgc actatcactt
     1501 ccctcatcat atcctagtat tatcagttag ttaaacacac aaagagatct gaacatctta
     1561 aataagacta tttaagatgc ataacttaga ttcgcaagat atcttccggt cattcaaaaa
     1621 caatttgcga tatagatatt ctcataccat gagatagtta tctgaaccac cctcaaccag
     1681 gtaagattaa atcagtgaaa cataatcata tttaataacg agaaatgcat tttaaaatgt
     1741 cttaatcaga attatagcaa atacagatta atcgcaattt ttcccagcaa aattacaaaa
     1801 tcatggtgta ttgacatcat caatcaccaa tgaaattatg caattatata cggataggga
     1861 ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata gttgctcaat
     1921 gcattgccaa ataatgccag aagagtcacc gcgtttgcag tactgcgcaa actcgtgttt
     1981 ttgtatgtgg cccgaagaaa gttcatattt taatcgtggt gtggtagaag gtatcttaac
     2041 aaaaaaccac aatgcgagat taagcggata tatttttgtc gatttttcag taagtttttt
     2101 acgtctattc cttgaaaaag actggattga ctatcttgca agtactgaca tggggattgt
     2161 tttagtcagc gaccgcaata tgcaatcgtt agctaattac tggcgaaaac acaactcagc
     2221 aatatctgct gtcatatata atgatgatgg tcttgatgtt gccaatgaaa agatcagaca
     2281 actatttatt ggtcgttatt tatcatttac cagaggtaac acattaactc agatggaatt
     2341 caccataatg gggtatatgg tttctggtta taatccatat cagattgctg aagttctgga
     2401 tatggatatc cgtagcatct acgcgtacaa gcaacgaatc gaaaagagaa tgggtggtaa
     2461 aataaacgaa ttatttattc gttcacattc ggtccaacat tgatactcaa cgaccagcca
     2521 gaatcatact ctgtttatac gggcatttat tgaaatgtcc gtattttata aataggccgt
     2581 cccctcaaga gtaaacacca tagtatttaa aaccatcaca cgaaaaattc agaagcatta
     2641 cgaaatatga at
//
LOCUS       DAZFWP020000309         2594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFWP020000309 DAZFWP020000000
VERSION     DAZFWP020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.311"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            359..1306
                     /gene="stxA1a"
                     /locus_tag="ACHK0Z_RS25415"
                     /old_locus_tag="pgaptmp_005083"
     CDS             359..1306
                     /gene="stxA1a"
                     /locus_tag="ACHK0Z_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4504926.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1316..1585
                     /gene="stxB1a"
                     /locus_tag="ACHK0Z_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             1316..1585
                     /gene="stxB1a"
                     /locus_tag="ACHK0Z_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HGU4504927.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2085..>2594
                     /locus_tag="ACHK0Z_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             2085..>2594
                     /locus_tag="ACHK0Z_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4504928.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
BASE COUNT          667 a          511 c          664 g          752 t
ORIGIN      
        1 attgcacatg gcactattag ttttctaaat attgtgtatt ttttgtattg caggatgacc
       61 ctgtaacgaa gtttgcgtaa cagcattttg ctctacgagt ttgccagcct cccccagtgg
      121 ctggcttttt tatgtccgta acatcctgtg tatcaataaa tgttgttatc tacgtacgtc
      181 aagtagtcgc atgagatctg accagatatg ttaaggttgc agctctcttt gaatatgatt
      241 atcattttca ttacgttatt gttacgttta tccggtgcgc cgtaaaacgc cgtccttcag
      301 ggcgtggagg atgtcaagaa tatagttatc gtatggtgct caaggagtat tgtgtaatat
      361 gaaaataatt atttttagag tgctaacttt tttctttgtt atcttttcag ttaatgtggt
      421 ggcgaaggaa tttaccttag acttctcgac tgcaaagacg tatgtagatt cgctgaatgt
      481 cattcgctct gcaataggta ctccattaca gactatttca tcaggaggta cgtctttact
      541 gatgattgat agtggcacag gggataattt gtttgcagtt gatgtcagag ggatagatcc
      601 agaggaaggg cggtttaata atctacggct tattgttgaa cgaaataatt tatatgtgac
      661 aggatttgtt aacaggacaa ataatgtttt ttatcgcttt gctgattttt cacatgttac
      721 ctttccaggt acaacagcgg ttacattgtc tggtgacagt agctatacca cgttacagcg
      781 tgttgcaggg atcagtcgta cggggatgca gataaatcgc cattcgttga ctacttctta
      841 tctggattta atgtcgcata gtggaacctc actgacgcag tctgtggcaa gagcgatgtt
      901 acggtttgtt actgtgacag ctgaagcttt acgttttcgg caaatacaga ggggatttcg
      961 tacaacactg gatgatctca gtgggcgttc ttatgtaatg actgctgaag atgttgatct
     1021 tacattgaac tggggaaggt tgagtagcgt cctgcctgac tatcatggac aagactctgt
     1081 tcgtgtagga agaatttctt ttggaagcat taatgcaatt ctgggaagcg tggcattaat
     1141 actgaattgt catcatcatg catcgcgagt tgccagaatg gcatctgatg agtttccttc
     1201 tatgtgtccg gcagatggaa gagtccgtgg gattacgcac aataaaatat tgtgggattc
     1261 atccactctg ggggcaattc tgatgcgcag aactattagc agttgagggg gtaaaatgaa
     1321 aaaaacatta ttaatagctg catcgctttc atttttttca gcaagtgcgc tggcgacgcc
     1381 tgattgtgta actggaaagg tggagtatac aaaatataat gatgacgata cctttacagt
     1441 taaagtgggt gataaagaat tatttaccaa cagatggaat cttcagtctc ttcttctcag
     1501 tgcgcaaatt acggggatga ctgtaaccat taaaactaat gcctgtcata atggaggggg
     1561 attcagcgaa gttatttttc gttgactcag aatagctcag tgaaaatagc aggcggagat
     1621 tcataaatgt taaatacatc tcaattcagt cagttgttgc cggtctgata atagatgtgt
     1681 tagaaaattt ctgcatggtg aatccccctg tgcggagggg cgactggtga acggtatgat
     1741 ctctttgatg atcgtaagcg agaatacgcg ggtttggtgg caccaggccg aactcaccgg
     1801 gaggcacccg gcatcatgct gtatacagag attaggcata tatccaggct cctcatcgca
     1861 ggagcctttt tacatgcaaa aaaaagcccg agtgggttcg ggcaacagca tgagatactt
     1921 gcattgtcat ttttatcgtg cggattttaa ccaggattca taaggctgcg caactgcgcg
     1981 gcctttttcg tatttcgggc tgtagtcccc gtgtgtcatt caggcttccg gactacagcc
     2041 cactccatat ctgatttaat acactatccc ggccgggagg aataatgaca tttaaacatt
     2101 atgatgttgt cagggcggcg tcgccgtcag accttgcgga aaagctgaca cacaaactga
     2161 aagagggctg gcagccgttt ggtagtccgg tggccataac cccttatacc ctgatgcagg
     2221 ccattgcggc ggaaggtgat gtcaccacac cagtggcggt gaccggtaat gagggtaagg
     2281 cggtggctgt cagtgccacc agcgacccgg agtattactt tgttgtggtt ctggcagggc
     2341 agtcaaatgg catgtcgtat ggtgaaggtc ttccgctgcc ggagacatat gaccgtccgg
     2401 acccgcgtat taagcagctg gcgcgccgca gtacggtgac accgggcggt gcagcatgca
     2461 aatataacga catcattccg gcggaccatt gtctgcatga tgtgcaggac atgagccgtc
     2521 ttaaccatcc gaaagcggac ctgtcaaaag ggcagtacgg taccgtgggg caggggctgc
     2581 atatcgccaa aaaa
//
LOCUS       DAZFWP020000310         2551 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFWP020000310 DAZFWP020000000
VERSION     DAZFWP020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.312"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHK0Z_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             complement(<1..245)
                     /locus_tag="ACHK0Z_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4504929.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..574)
                     /locus_tag="ACHK0Z_RS25435"
                     /old_locus_tag="pgaptmp_005087"
     CDS             complement(245..574)
                     /locus_tag="ACHK0Z_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4504930.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(571..>2551)
                     /locus_tag="ACHK0Z_RS25440"
                     /old_locus_tag="pgaptmp_005088"
     CDS             complement(571..>2551)
                     /locus_tag="ACHK0Z_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4504931.1"
                     /translation="QFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSGLMAMARQ
                     FRNVTAEQIAYVAQLQRSGDEAGALQAANDAATKGFDEQTRRLKENMGTLETWADKTG
                     KAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQ
                     GAWDDRENARLGLAAATLQSDMEKAGELATRDRAERDASQLKYTGEAQKAYERLLTPL
                     EKYTNRQEELNKALKDGKILQADYNTLMAAAKKDYESTLKKPKSSGVKVSAGERQEDL
                     THAALLALETELRTLEKHSGANEKISQQRRDLWKAESQFAVLEEAAQRRQLSAQEKSL
                     LAHEQETLEYKRQLADLGDKVEYQKRLNELANQAVRFEQQQSAKQAAISAKARGLTDR
                     QAQRESESQRLRDVYGDNPDALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESA
                     TDSFSQVKSVATQTFDGIAQNMAAMLTGSEQSWRGFTRSVLSMLTEIFLKQAMVGIVG
                     SIGSAMGGAFGGGASASTGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTK
                     EATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGISFNQNNHVVIQNDGTNGQA
                     GPQLMKAVYDMARKGAQDEIQAQMRDGGVFSGGRR"
BASE COUNT          469 a          900 c          597 g          585 t
ORIGIN      
        1 gacaccgtca gcgacgggcg ggcactgctg cccttcccgt tcatctcaaa gccactgccg
       61 tcaatcgggt atgcctgata ttgccgcccc tgccaggtaa ccgcctcccc tttttcattc
      121 agctcattgc agaaaaaata ccgctcacca ccctgtaccg tcaggtcgat ttcccagagt
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca gactttcttc gtgaatatcc
      241 tgcatcagtt caccacctgc ttaaactccg cgctgaattc aacccgcagc atgcgaaccc
      301 gtgatgacca ggcggcacag gtcaccttta tctgccgcca ggcataaggc ggtgtccaca
      361 gaaacgcctt ccagccaccg tgctctgcca ggaatgcctc cagatgtcgg gcctcctccc
      421 gggtcacgga aagcgtcaca cggtatgttt tcaggtcagc attcagcccc gccgccatac
      481 gctgcgaata cccgtcacca aaacgcactt cacgcaccga tggctgcgag ttcacctcca
      541 tatccggctt cactttccag cgaaatgttt tcatcgcctg cctccggaaa agacgccgcc
      601 atcacgcatc tgcgcctgaa tctcatcctg cgcccccttg cgggccatgt catacaccgc
      661 cttcatcagc tgcggccccg cctgtccgtt ggtgccgtcg ttctgaatca ccacgtgatt
      721 gttctgatta aaactaatgc cttccgcccg ccgcatctgc gccggacttc cggcaccgcc
      781 cacataacca ccttccgcat acccgcgcat cagacgatac aggttgccga cgccaatccg
      841 gctggttgcc tccttcgtga agacaaactc cccgcggtgg acaataccgg caggttcgta
      901 tttgccaccg gttcccgtaa atcctccggt cgcgaaatgg aagttcgccg ccgcagcctg
      961 aatggccgtc cccgtggagg cagacgcccc accaccgaaa gcaccaccca tggcgctgcc
     1021 aatactcccg acaatcccca ccatggcctg cttcagaaaa atctctgtca gcatggagag
     1081 cacagaacgg gtgaaaccac gccagctctg ttcgctgccg gtcagcatcg ctgccatatt
     1141 ctgtgcaata ccgtcaaagg tctgcgtggc cacgctttta acctgcgaaa aactgtccgt
     1201 cgcactttct gcccactcgc cccagccgga cttcagaccg gccatccagc ttccacgaag
     1261 ctgctcctcc gcagaccagg tgttcttcag tgcagatgtg gccttcgcca gcgcatccgg
     1321 attatcaccg tacacgtcac gaaggcgctg cgattccgac tcccgctgtg cctgacggtc
     1381 cgtcagcccc cgggcttttg cgctgattgc agcctgcttc gcactctgct gctgttcaaa
     1441 ccgcacagcc tgatttgcca gctcattcag ccgtttctgg tattcaacct tgtcacccag
     1501 gtcagccagc tggcgtttgt actccagcgt ctcttgctca tgggccagca gggatttctc
     1561 ctgtgcagac agctggcgac gttgcgccgc ctcctccagt accgcgaact gactctccgc
     1621 cttccacaaa tcccggcgct gctggctgat tttctcattc gctccgctgt gcttctccag
     1681 cgtccggagc tcggtttcaa gcgccagcag cgccgcatgc gtcaggtctt cctgacgctc
     1741 accggctgac actttaacac ctgacgactt cggctttttc agcgtcgatt cataatcctt
     1801 tttcgccgcc gccatcagcg tgttgtaatc cgcctgcagg attttcccgt ctttcagggc
     1861 cttattcagc tcttcctgcc ggttagtata tttctccagc ggcgtcagca gacgctcata
     1921 cgccttctgc gcctctccgg tatacttcag ctgtgatgcg tcccgttcgg cccggtccct
     1981 ggtagccagt tcaccggctt tttccatatc cgactgcagc gtggccgctg ccagccccag
     2041 acgggcattt tcccggtcat cccatgcgcc ctgcaggttg gcacggaaag aggaggtctt
     2101 tccccggcgc tggctccggc tctggtacca ctgccatttt ttatccgcct catcgaatgc
     2161 cttctgcgca ctggcaagca tatccgctga ggattccgga cgaccaatat ccagaatggc
     2221 atcccacatc gatttgaacg ccttccctgt tttatccgcc caggtctcca gcgtccccat
     2281 gttttctttc aggcgacggg tctgctcatc aaagcctttc gtggcagcat cgttcgccgc
     2341 ctgtaaggcc ccggcctcat caccggaacg ctgcagctgc gcaacatacg caatctgctc
     2401 tgccgtcacg ttacggaact ggcgcgccat cgccatcagc cccgacgtcg ggtcggtggt
     2461 cagttttccg aaagcctctg caaccttgtc cacctccaca ccggatgcag aagcaaaacg
     2521 cgcgacactc tggttgatgg catcaaactg t
//
LOCUS       DAZFWP020000311         2507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFWP020000311 DAZFWP020000000
VERSION     DAZFWP020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.313"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..112
                     /locus_tag="ACHK0Z_RS25445"
                     /old_locus_tag="pgaptmp_005089"
     CDS             <1..112
                     /locus_tag="ACHK0Z_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4504932.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            171..374
                     /locus_tag="ACHK0Z_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             171..374
                     /locus_tag="ACHK0Z_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4504933.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            374..1424
                     /locus_tag="ACHK0Z_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /pseudo
     CDS             374..1424
                     /locus_tag="ACHK0Z_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1452..1541)
                     /locus_tag="ACHK0Z_RS25460"
                     /old_locus_tag="pgaptmp_005092"
     tRNA            complement(1452..1541)
                     /locus_tag="ACHK0Z_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Ser,seq:cga)
     gene            1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHK0Z_RS25465"
                     /old_locus_tag="pgaptmp_005093"
     CDS             1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHK0Z_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU4504934.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
BASE COUNT          745 a          529 c          579 g          654 t
ORIGIN      
        1 ggctattcca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaggcaaa
       61 atacgtttca gttatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 cctaattgcc gctaaccgta tatagttagc ggcggttatg agatatagct atgagtagct
      181 tatttttaac cgaagatgaa ttgctaatat taacgggctg caaatatgca agccaccagc
      241 gaaaatggtt aacggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg
      301 tcagccggga tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt
      361 ttggtgcaat ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaac tacctccaaa
      421 tgtataccca aataaatata gttatgtatg gaaaccaaca tccagagaat ctgtcacact
      481 aaccgccatc aaggatggtt tagctgcttt atggaaaagg tatgaggaaa ctgtaaataa
      541 tcgcgatcgc gcaatgacat tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta
      601 cagtgacctc agtccaagaa cacaaaaaga ttatctacaa catcaaaaaa agttgcttgc
      661 cgtattcggt aaggtgccgg cagattccat aaaaccagaa cacatccgtc gatacatgga
      721 caagagaggg gagcagagta aaacgcaagc caaccatgaa aaaagcagta tgtcccgcgt
      781 ttacagttgg gggtatgagc gagggtacgt gaaaggtaac ccatgtgcag gtgtaagtaa
      841 attcaaggcc aaaaaccgcg aacgatatgt aaccgacaaa gaataccagg cagtattaag
      901 cgttgcacct cttcctgttt ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt
      961 ttccgatgtg ttatcgctga aatgggagca gattggaaac gacggaatct ttatccagca
     1021 agggaaaaca ggaaaaaaac agataaaagc atggagtcca cgattacagg cggcgatcga
     1081 aaaagcaaaa cagttaccaa catccgccta tgtaatcagt aatcaatacg gcaaccgata
     1141 tatgtacaaa ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc
     1201 aggtatttta accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga
     1261 aggaagcagt cgggataagc aacttttctc tggtcacaaa accgaggggc aagtgctaat
     1321 ctatgacagg aaggttaaag tttcaccgac acttgatgtc ccgttacctg aaaatattcc
     1381 aagaaaatat tccaagtaat tccaagtgtg atttttgtca ctgacttaat gatgtataag
     1441 tgattgaatt ttggcggaga gagggggatt tgaacccccg gtggagttgc ccccactccg
     1501 gttttcgaga ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga
     1561 tgccaggaaa tttggcattt taacagtccc tgtccgtgca attttgttca agtgacgagt
     1621 ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg
     1681 cccttccctg gcaggaagca ctatcgatcc cccttttaac gtgtctgacg gaacaggaac
     1741 aaagcaaatt agtcactctt gccgaacgtt ttttacagca aaagcggctt gttcctttac
     1801 agggctttga actggattca ttaagaagct gccggatagc acttctattt tgcctacccg
     1861 ttctggagtt aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc
     1921 catttgtggt cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg
     1981 ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag
     2041 attcttttga tgcttctggt tttaacctga ttattcatga agtcgctcat aagctggaca
     2101 cccgtaacgg cgatcgcgcc agcggagttc cctttattcc gttgcgtgag gttgctggct
     2161 gggaacacga tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttggttggcg
     2221 agaatgcggc gagcattgat gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac
     2281 tttctgaata tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc
     2341 aacgtttctg ccaattttat caacaagatc ctttgcagag actgcatcgc actaatgata
     2401 cagactcgtt ttcggcgacg aatgttcatt aattaacaac tttgcagatt aattaaccaa
     2461 ttgaaatgac ttatgaaatt tagtgttgac agacaaggta ccgctaa
//
LOCUS       DAZFWP020000312         2502 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFWP020000312 DAZFWP020000000
VERSION     DAZFWP020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.314"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            66..389
                     /locus_tag="ACHK0Z_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             66..389
                     /locus_tag="ACHK0Z_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4504935.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            395..1531
                     /locus_tag="ACHK0Z_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             395..1531
                     /locus_tag="ACHK0Z_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4504936.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1528..>2502)
                     /locus_tag="ACHK0Z_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             complement(1528..>2502)
                     /locus_tag="ACHK0Z_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4504937.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          611 a          580 c          632 g          679 t
ORIGIN      
        1 tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata ttctctgagg
       61 gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt ctggtgatct
      121 tctatgggat tattatttta catgatattc cctacctgat tgctaaaaaa cgtaatcatc
      181 ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgtta catgttatct
      241 ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg ggaatgcaaa
      301 accatgactc ctccgttgtg cagctacaac agcgtattgc cgggctggag aaacagctcg
      361 ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta ttttgaccta
      421 tgtggctttc gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata aatggaccat
      481 tcccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt taatgaacta
      541 taaccatccg tataccttca aagcgcaaaa agcggttatt tctattcctg ttgtcccaca
      601 ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta aaaaaggtga
      661 ggtgctattt cgactggacc cgacgcgtta tcaggcgcgg gtggatcggc tgatggcgga
      721 tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg agatggcggc
      781 gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc agcgttacgc
      841 acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg tggcgcggca
      901 aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa aacagatcca
      961 gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc tgaaagcgca
     1021 gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga gcgatggtta
     1081 tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc tacgtccggt
     1141 gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc agaactcctt
     1201 gctgcgactg gcccctggcg atgatgcgga agtggtattt aacgctctgc caggtaaggt
     1261 attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt atcagtcgac
     1321 cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg cgaccattga
     1381 actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc aggtggcggt
     1441 ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctgttac gcatgaccag
     1501 ttgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt gttcaggccg
     1561 cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat ccagcacata
     1621 ctggacccgc cagggctggt attcccgttt acggaagata gccaccagat ccagccacca
     1681 ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcaggtcgc tctgcacact
     1741 tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg ccgattgcgt
     1801 gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac tcccggcgac
     1861 gttgaagcgc cagatattcg cgtcgtcaat catcatcgag tcgatcaaaa tacaggagtg
     1921 atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct ggctggcata
     1981 agcggttacg gcatattggg taataaaact ggcaaccagc gattcatctt ttggtggagc
     2041 gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa aggcattacg
     2101 ctcaagattg caggttttta gcgataaggt gatatcaccg atatctttaa ttttatcgat
     2161 tacatgtttg gataaatagg taattatgcc ggttggggcg tagatagtga cccgaccacg
     2221 tttctcatgc ttataatctg caataaaatt attaagttgc tcgtttctat ccagcatgtc
     2281 gttgatgtac ggtaacagcg cggtgccaaa aggtgtgagc atcagctgcc gggtggttcg
     2341 gtcaaagact tttaaaccca cttttgattc aaaatcagca agatatttgc tgacgttggc
     2401 ctgtgcgatg ccaagtacag tcgcggcatg gctgatattt tcactggcag cgattaccga
     2461 gataattttt aactcccggt acttaagttg taattttgtc at
//
LOCUS       DAZFWP020000313         2453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFWP020000313 DAZFWP020000000
VERSION     DAZFWP020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.315"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..333
                     /locus_tag="ACHK0Z_RS25485"
                     /old_locus_tag="pgaptmp_005097"
     CDS             <1..333
                     /locus_tag="ACHK0Z_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504938.1"
                     /translation="NMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGG
                     LLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTQSEAVLQKSLDITEEADLAL
                     RNAMARIK"
     gene            673..1491
                     /locus_tag="ACHK0Z_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             673..1491
                     /locus_tag="ACHK0Z_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4504939.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1582..2067
                     /locus_tag="ACHK0Z_RS25495"
                     /old_locus_tag="pgaptmp_005099"
     CDS             1582..2067
                     /locus_tag="ACHK0Z_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4504940.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            2083..>2453
                     /locus_tag="ACHK0Z_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             2083..>2453
                     /locus_tag="ACHK0Z_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4504941.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLIT"
BASE COUNT          584 a          657 c          655 g          557 t
ORIGIN      
        1 aatatggtga ttgtgacgca tctgagtgac ggttctttat gggatcgcca ggcttttccg
       61 gacaccacaa tccttgagat ccgtccccgg aaaaggctga aatatgcagg tgatggtggc
      121 aacagcggcg gtctgctcag ttttacatcg gcacataccg acgcctggcg tcagcagggc
      181 tatgaagaca cgatgctggc gatggagcat atccggaaac cgctggcagc acgtcaggca
      241 ctgacccagt cagaggcggt attgcagaaa agcctggata taacggaaga ggcagatttg
      301 gcactgagaa acgcgatggc ccggattaaa taaagacgcc ccaaagaaac gccaccgcct
      361 gtggtggtgt ttttcctgtc tggtgttatt gaaaatattt tcagatatat attatctggg
      421 tgatggttaa gatgaattta tttaattttg ggggaagata caaaaacaac cagtgaacat
      481 atttccggcg ggttatccgc aatgctgaag gtcagttgca ctggcgatgc cggaatacag
      541 cttaatacgt ggttcgccag tgaagggctt ctcagacaat aaacctcatt catttcaccc
      601 atcaggccgc gtcttctccg ggagacgcgg ctttttcatt tatatcgctt aatcattcat
      661 aaggagcaaa tcatgagatt agccagtcgt tttggttatg cgaaccagat acgtcgtgat
      721 cgtccgctga cacacgaaga actgatgcgc catgtaccga gtatttttgg ggaaaaccga
      781 catacctctc gcagtgaaca ctatgcgtac attcccacta tcaccgtcct ggaaaatctg
      841 cagcaggaag gctttcagcc gttctttgcc tgccagaccc gcgtgcgcga ccagagccgc
      901 cgggaataca caaaacacat gctgcgtctg cggcgggccg gacagataac cggtcagcat
      961 gtccctgaaa ttattctgct caactcccat gacggttcat ccagctacca gatgttaccc
     1021 ggatattttc gtgccatttg taccaatgga ctggtctgcg gtcagtctct gggagaattg
     1081 cgtgttccac accggggaaa tgtggtggac agagtcattg aaggggctta cgaggtggtg
     1141 ggcgtttttg accggataga ggagaagcgt gatgccatgc agtcgctgat tctgccgcca
     1201 ccggcacgcc aggcgctggc acaggcggca ctgacttacc gttatggtga cgaacatcag
     1261 cccgtcacca ccgccgacat tctgacacca cgacgccggg aggattacgg taaggacctg
     1321 tggagtgctt atcagaccat ccaggagaat atgctgaaag gcgggatttc cggtcgcagt
     1381 gcaaaaggaa aacgtatcca tacccgggcc attcacagca ttgataccga cattaagctc
     1441 aatcgcgcat tgtgggtgat ggcagaaacg ctgctggaga gcatgcgctg atgccgtttc
     1501 actgaaagtg cagtcctgac tcacatctgt tcctgccccg gggcttcttc cattcattta
     1561 ctttttataa ggaataacct catgacaacc gtttcgcata attccaccac accttctgtt
     1621 tccgtaacca ctgcatcagg gaataaccca cctcagctgg ttgccactcc cgtccctgat
     1681 gaacagcgca tcagcttctg gccgcagcat tttggcctca ttccacagtg ggtgaccctg
     1741 gagccccgtg tcttcggctg gatggaccgt ctttgcgaag actactgcgg tggtatctgg
     1801 aatctgtaca ccctgaacaa cggcggggca tttatggcac ccgaaccgga tgacgatgat
     1861 gacgaaacat gggtactgtt caatgccatg aacggcaacc gcgctgaaat gagcccggaa
     1921 gccgccggta ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg tacggagtgt
     1981 tatgccatga cggtccacta ttaccggctg cgggattacg ccctgcagca tccggaatgc
     2041 agcgccatta tgcgcatcat tgactgaatg gaggcaggga caatgcagca gctttccttt
     2101 ctgcccggag agatgacgcc cggcgagcgc agtctcattc tgcgggccct gcaaaccctg
     2161 gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc acgggaatgg
     2221 ctgattctga acatggcggg actggagcgg gaagagttcc gggtgctgta tctgaacaac
     2281 cagaatcagc tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg tacggaagtt
     2341 catccccggg aagtgattaa acgcgccctg taccacaatg ccgctgccgt ggtgctggcg
     2401 cacaatcacc cgtccggtga agtcacaccc agtaaggcag accggcttat cac
//
LOCUS       DAZFWP020000314         2449 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFWP020000314 DAZFWP020000000
VERSION     DAZFWP020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.316"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..523
                     /locus_tag="ACHK0Z_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             <1..523
                     /locus_tag="ACHK0Z_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGU4504942.1"
                     /translation="RATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFA
                     ISVINDKATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVR
                     IEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDN
                     EIITSAIDKLKRS"
     gene            551..1509
                     /locus_tag="ACHK0Z_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /pseudo
     CDS             551..1509
                     /locus_tag="ACHK0Z_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1521..1982
                     /gene="yibG"
                     /locus_tag="ACHK0Z_RS25515"
                     /old_locus_tag="pgaptmp_005103"
     CDS             1521..1982
                     /gene="yibG"
                     /locus_tag="ACHK0Z_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4504943.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          779 a          441 c          561 g          668 t
ORIGIN      
        1 tcgagctacg gcaatcgagt caacggctca gcgatgcaag aaaaacccaa tttattcacc
       61 taaaatagta gaacaatctc aaattactgc ttttgataaa tcgactaatg tcagacgtgc
      121 tacagcattt gctatttctg ttatcaatga taaagcaaca attccactat tgattaatct
      181 gttaaaagat ccaaatggag atgtcagaaa ctgggccgca tttgcaataa atatcaataa
      241 atatgataat agtgatatta gggattgttt tgtggagatg cttcaggata aaaatgagga
      301 agtccgtatt gaagcaataa tcggactttc ctacagaaaa gataaaaggg ttttatctgt
      361 tttatgcgat gagttaaaaa aaaatactgt ttatgatgat atcattgaag ctgcgggtga
      421 attaggtgat aaaacgctac ttcctgtttt agatactatg ttgtacaagt ttgatgacaa
      481 tgaaattata acttccgcta ttgataagct gaagcgttca tagcgtgagt tgcctatgca
      541 cagtggggga ttcccgccgg cacggtgcag atgctcgacc ggctggaaag tgaaatcctg
      601 gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct gaccgtggag
      661 cagatgaaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca cctgtaccac
      721 tgcgaccatc gcggcctgcc gctggcgctc atcagcacgg aaggggcaac agcgtggtgc
      781 gcagaatatg atgaatgggg caacctgctg agtgatgaga acccgcatca tctgcagcag
      841 ctcattcgcc tgccgggtca gcagtatgat gaggagtccg gcctgtatta caaccgccac
      901 cgctattatg acccgctgca ggggcggtat atcactcagg atccgattgg gctgaagggg
      961 ggatggaatt tttatcagta tccgttgaat ccggtcataa atgtagatcc gcaaggtttg
     1021 gttgatataa atttataccc cgaaagtgat cttatccatt ctgtagctga tgagattaat
     1081 atcccaggcg ttttcacaat cggggggcat ggtaccccca catctattga atccgcaacg
     1141 cgcagtatca tgacagctaa agatctagca tatctaatta aatttgatgg gaattataaa
     1201 gatgggatga cagtttggtt attttcttgt aatacaggta aaggacaaaa ttcatttgct
     1261 agccaattag ctaaagagtt acatacaaat gtaataggac ctgacacgct atggacgtgg
     1321 tgggggcgag gaactaatgg taagttaaaa atggatacag tgctaacagc accaacgaac
     1381 cttaattcaa ataaggatct aatggctata acaacaaaag accttggtaa ttggataaca
     1441 tatgggccat ctgggcaccc catttctaat atgcaaggta cgccagaaaa acccagtgat
     1501 ataagatagg ttgtagatgt atgaaagcat gcttgttact atttttttat ttttctctta
     1561 ttggtcaatt gcatggtgct gatgtgaaaa taaaagaaaa cgaaagtgtg atgggatcta
     1621 cagcaatgac ctatgattta agtgaagaaa agctgatgaa actgaagtat aaatcacaac
     1681 atggtgattc agaggcatca tttcgactat atcaatatta ctgctttact aaaaataata
     1741 ttgataaaca attgcgattc ttggaaagat cagcatctca ggggaatgtt acagcgcagt
     1801 ttaattatgg ggtcttttta tcagatacaa atccaacatt atcagaatat tataatttga
     1861 atagagctat ttattggatg gaattcgctg taaataacgg taatattgat gcaaagagca
     1921 aacttcaaga gctcaaaaag ctaaagcgaa tggatagaag gaagaataag gagaatccgt
     1981 aaaagcaata gttcatcaga ctgccgcagc agtagtatga tgatgaatca gggctgtact
     2041 ataactatta tcggtaacat taaccgcagc aggggcggta taacactcag gctccgattg
     2101 ggctggaagg ttggtggaat ttttatcagt atccgttgaa cccgatttct ggaatcgatc
     2161 cccttgggat tagccacctg tttatacagt ataactttag gtatgctttc gtgtgtttca
     2221 gatataccat gtgatgataa tttttatgat gtgcttagta ttccagtcgc ttctggtaat
     2281 aatatgcaat gtaaaaataa tccccgttgt acacatttat aaatagatgg ccaatacctc
     2341 aaggcctatg gagttagaat gttaatgacc cgggagcaag caatagaaaa cctgacggca
     2401 tacgattagt accgtcagtt aatacaggaa cttataatcg caatggatt
//
LOCUS       DAZFWP020000315         2424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFWP020000315 DAZFWP020000000
VERSION     DAZFWP020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.317"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(90..1502)
                     /gene="pykF"
                     /locus_tag="ACHK0Z_RS25520"
                     /old_locus_tag="pgaptmp_005104"
     CDS             complement(90..1502)
                     /gene="pykF"
                     /locus_tag="ACHK0Z_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU4504944.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMTSRLDFNNDSRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAQKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1510..1704)
                     /locus_tag="ACHK0Z_RS25525"
                     /old_locus_tag="pgaptmp_005105"
     CDS             complement(1510..1704)
                     /locus_tag="ACHK0Z_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504945.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2059..2268
                     /gene="fumD"
                     /locus_tag="ACHK0Z_RS25530"
                     /old_locus_tag="pgaptmp_005106"
     CDS             2059..2268
                     /gene="fumD"
                     /locus_tag="ACHK0Z_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGU4504946.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          652 a          522 c          568 g          682 t
ORIGIN      
        1 ttgatttttt attgcttctg gttatcgatt aaataaaaaa agcgcccatc agggcgcttc
       61 gatatacaaa ttaattcaca aaagcaatat tacaggacgt gaacagatgc ggtgttagta
      121 gtgccgctcg gtaccagtgc gccagaaacc atgaccacaa catcaccttt ctgagccaga
      181 ccgctctgca aagctagttc tttacccaga cggtagaagt catcagtaga ggtgatttct
      241 ttcaccaact gcggcacaac gcctttgctc agcaccagct gatgtgcagt tttttcgttg
      301 gtggtcagcg ccaggatggt tgcatccggg aagtatttac gcaccgcacg tgcagattta
      361 ccgccctggg tcgcaacaac gatcagcgga gcatccagtt tttctgcagt ttcaactgca
      421 ccacggcaaa ctgcttcagt gatacgcagt ttacggctgt cgttgttgaa atccagacgg
      481 ctggtcatta cacggtcagt acgttcgcaa atggtcgcca taatagaaac agcttccagc
      541 ggatacttac cttttgcgga ttcaccagac agcataactg cgtcagtacc atccagaatg
      601 gcgttagcaa cgtcacctgc ttctgcgcgg gttggacgtg ggtttttgat catggaatcc
      661 agcatttgag tcgcagtaat aacgacttta cgtgcacgga tacatttttc gatcatcatc
      721 ttctgagcga agataacttc ttcaaccggg atttcaacac ccaggtcgcc acgcgcaacc
      781 atgataccgt cagacgcttc gaggatttcg tcgaagttgt tgaggccttc ctggttttcg
      841 attttggaga tgatgtggat gttttcgccg ccgtgcgctt tcaggtgctc acggatttcg
      901 ataacgtcag aacgcttacg aataaaggaa gcagcaacaa agtctacgcc ttgttcgcaa
      961 ccaaagatca ggtcctgttt gtctttttca gccagtgctg gcagagcaat ggaaacgcca
     1021 ggcaggttca cacctttgtt ttcgcccagg tcaccgttgt tcagcacttt acagataact
     1081 ttgttacctt caatggcggt aacttccata ccgatcagac catcgtcaac cagtacggtg
     1141 ttgccaacag acaggtcagt agtgaaacct tcatacgtta ccgcaaccat ttcgctgttg
     1201 ccgataacag atttatcagt ggtgaaagta aaggtctgac cagctttcag agaaacgtcg
     1261 ttaccgcctt ccagtttcat ggtgcggatt tccggacctt tggtatcaag caggatagcg
     1321 gcggttttac cagttttgct catcacgttg cgcagattct gaatgcgctg accgtgttct
     1381 gcatagtcac catgagagaa gttcagacgc ataacgttca tgccagcgtc cagcatttta
     1441 gctaacatct cttcagattc ggttttcggt ccgatggtgc aaacaatttt ggtctttttc
     1501 atgacagtct tagtctttaa gttgagaagg atgggagaaa cttgctttct gggcgcactg
     1561 ccggaaagcg aatccggtat tacgaaagtg gtggtgcctc gctctaagga taggtgacat
     1621 cgaaaaagcg tgcagaggaa tgtgtgcttg tgtctcagcc caacgtgagt gcctgttttt
     1681 gagtcgttga gttctacagt ccaaagtgct gaaaccattc aagagtcaat tggcgcgcat
     1741 tatacgctga aattaatcca aaaggaaaac aaaaacagcg tttcaaaaaa tatacaactt
     1801 gttagacaat aaattgttat caaagattta acgttccagg gaaaaggtta cgcaaaaact
     1861 tcactgtttg cgcaacgtct ggtgccgaag ttgtcagtgt gaggataagt gaaagttcat
     1921 ccggtgtata aatatatcat cgctcaattg ctacagttaa aataatttat cgcgttattt
     1981 gtctgatttt gtttgatttt tgtttaattg gttagattga tatatgctga tagttggcat
     2041 gataaatagg tataaagaat gggaaatcgg acaaaagagg atgaactgta ccgagaaatg
     2101 tgcagagttg tcggtaaagt tgtgctggaa atgcgcgatc tggggcagga accaaagcat
     2161 attgttatcg caggcgtatt acgtacagca ttagccaata aacgcattca gcgcagtgag
     2221 ctggaaaagc aggcgatgga aaccgtcatt aacgcactgg taaaatgacc attcaggatg
     2281 aggcggtaag cttcatcctg atattcttgc ttcagttgcg gtccagttaa tcattctaaa
     2341 taacaactgc tgaaattata cgaatttcgg tatgtatttt aatttatagc gataatgtta
     2401 tttttatttt ctgcaatgaa cgtc
//
LOCUS       DAZFWP020000316         2401 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFWP020000316 DAZFWP020000000
VERSION     DAZFWP020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.318"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..389
                     /locus_tag="ACHK0Z_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             <1..389
                     /locus_tag="ACHK0Z_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504947.1"
                     /translation="RLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSI
                     FEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTG
                     YAERYQAIASQYSSRIGVDYVSMMLP"
     gene            complement(461..877)
                     /locus_tag="ACHK0Z_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             complement(461..877)
                     /locus_tag="ACHK0Z_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504948.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(916..2145)
                     /locus_tag="ACHK0Z_RS25545"
                     /old_locus_tag="pgaptmp_005109"
     CDS             complement(916..2145)
                     /locus_tag="ACHK0Z_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGU4504949.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDSDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
BASE COUNT          655 a          471 c          494 g          781 t
ORIGIN      
        1 taagattaag tccgcacagg ctcattaaag ttctggagct ttttaacgcc agtcagagta
       61 aagcattgcc atttgctgaa cattcgaaat atatatatgt aatgcataaa aatcaaagaa
      121 aatatctctc tattttcgaa ggggataaaa cgcttcctgt tgttgattat atggtggtgt
      181 tgaatcatgg aacaacagtt gatggcgaag aaaaaaatat tatttctgcc gtgtttttaa
      241 gtaatatgga tggcaacgtg caaaatgttc ctgtccgact caaacttaaa tctcaactga
      301 gaactggtta tgcagaaaga tatcaggcta tagcgtctca gtatagctca aggattggtg
      361 tggattatgt atcaatgatg ctaccataat tattatattt taggcgtggt gatttttttt
      421 cgccatgcct attttatatt tatcatcaca atgatgttgt ttatttttgt ttaagtcttc
      481 taagcttcat gcattctaat gagaataata atagagttgt gctgtatata aagccagagt
      541 tagttaataa ctttaaattt aatgcttcag gattttcact gagaaagtga aaggcataaa
      601 ctaagtatac agctaaagta gccgttccaa ttgttggtcc aatatcgcct tgatcatgag
      661 gttcaaggtt tacattgaac ttgaagatta gccattcaaa tccccaagta aaaaaaacaa
      721 ctaatatcat ggctgcgaat aatttcgcga tgttatctgc tgatggattc atcagaacag
      781 attgtttctg aaaaaattca caaagtgaga gtccgaaaaa aataaaaaaa agtggtctgg
      841 aactaaattt ttcaaaaact ttgaataggg tttccatttt ctttaccttc aggattatat
      901 tagctattct gatacttagg ctaccagagc atttgatctt tgagcttgaa cgtaatcact
      961 ccaccattgc atcaaactct gtcgctctat cagatattct gcacgattgt atgctgcgat
     1021 aatttcatct tttttcgagt gggcaagcgc tgcctcaaga acttcagctc tgaatttacc
     1081 agactcctct gccgctgttc gtgcaataga acgcataccg tgagctacaa gctcgcctcc
     1141 gaaccccatt cggatgatag ctgcgttggc tgtttgttca tgcatatgat taagaggcgc
     1201 ttttatgctg gggaaaaccc attctctatg cccacttatt gatttcatta attcaaggat
     1261 gcgcaaagct tctttactca aaggaacttt gtgaagcttt ttcattttca tgaaatcagc
     1321 aggaatgttc caaatgctgt tggttgtatc aatatcagac caccttgcgc gaacggcttc
     1381 acccggacga acccatgtca acaattgcca ttcaattagc atacgtgttt ccaaccggat
     1441 tgacgcattc gtcaaagatt ccataaacct tggcaattcg cttgggggaa gggcaggcat
     1501 attttgcttt tttggtttac tgaatctttg accaaggttg tcagccgggt tgaactcaat
     1561 aagttcttca gtagctgccc accggaagat ttcattcaga cgtgaaatga tacggcgtag
     1621 agtttccaat acgcctcgtt gctcaatagg atcaaggtgt tgttttaaga gcttaggtcg
     1681 gatctcattg atagggacat tacccagacc gggaaagaca tttctctcta agctgcgcca
     1741 gatgtctgct gcatggtctt gtgagatacc tgatgtcttt accttctcat ctaaccattt
     1801 ccgcgctacg gcttggagag tgtgctcagt agcactcttt aatgccttcg ccttatcgtt
     1861 gttatggatt tggggatcaa caccatttgc cagaaaggag agatattcat cacgtaaggc
     1921 tctggctctt gcaagggtaa ggtgaggata tgtcccaagg ctcattttgg ttcttttctt
     1981 gctcactggt actgcatacc tgaaatacca atttttcttg cctcctttcg ccaaaggagc
     2041 gattcgtaga atcagaccat cactgtcaaa caagttgatt tctttatcgg ctggcttggt
     2101 gcttttgatt tcagtgtcag tgagcttctt agcgattttt gccattttgg gaccctcggt
     2161 ttttggaccc ttcttagtgg gtcccattca gggtgccata actcgtagtt ctcagcaatt
     2221 ctcactggac gacaatagac gtaaaaaagc ccgcagagct tgtgctgtgt gggcttagta
     2281 gacttcattg aacttcaaac aactaaaaag tggtggagct ggcgggagtt gaaccagcgt
     2341 ccatttgttt ttaatttatt gatttttatt gttttatttt atttctcgca tgcttcgtgt
     2401 a
//
LOCUS       DAZFWP020000317         2387 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFWP020000317 DAZFWP020000000
VERSION     DAZFWP020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.319"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..276
                     /locus_tag="ACHK0Z_RS25550"
                     /old_locus_tag="pgaptmp_005110"
     CDS             <1..276
                     /locus_tag="ACHK0Z_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4504950.1"
                     /translation="VNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGGATVTPKLNG
                     VAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLMLEINAPAA"
     gene            568..783
                     /locus_tag="ACHK0Z_RS25555"
                     /old_locus_tag="pgaptmp_005111"
     CDS             568..783
                     /locus_tag="ACHK0Z_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4504951.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            788..1132
                     /locus_tag="ACHK0Z_RS25560"
                     /old_locus_tag="pgaptmp_005112"
     CDS             788..1132
                     /locus_tag="ACHK0Z_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU4504952.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1183..1716
                     /locus_tag="ACHK0Z_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             1183..1716
                     /locus_tag="ACHK0Z_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4504953.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGTTRVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDLKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1872..2054
                     /locus_tag="ACHK0Z_RS25570"
                     /old_locus_tag="pgaptmp_005114"
     CDS             1872..2054
                     /locus_tag="ACHK0Z_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504954.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            2067..2198
                     /locus_tag="ACHK0Z_RS25575"
                     /old_locus_tag="pgaptmp_005115"
     CDS             2067..2198
                     /locus_tag="ACHK0Z_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504955.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            2206..>2387
                     /locus_tag="ACHK0Z_RS25580"
                     /old_locus_tag="pgaptmp_005116"
     CDS             2206..>2387
                     /locus_tag="ACHK0Z_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4504956.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDV"
BASE COUNT          611 a          490 c          699 g          587 t
ORIGIN      
        1 gtgaatctga tgcatcacag gaaagccgga aacacgaagc tgggggagtt cggcgattac
       61 ggtaacgact ggcagacgct ggagctggtg ttcaccgccg gcggtgccac ggttactccg
      121 aaactgaatg gagtggctgg cccggcattc caggttataa aagacagtct gacactggga
      181 ctgaatgcgc tgacgctgac ggatgttaca aaaaatgcag cgtatggcgt tgagatagaa
      241 agtctgatgc tggagataaa tgcaccggca gcataataaa aaaacagcca gtactgactt
      301 tcgtcggaga agtactggct aagaaggata gttgggtttc acatgatact tatatctggc
      361 agtacatttt ctgacagaca gtgatgggtg ttgtcaagat attgtgtcat ttataacctg
      421 aatcaggggg ggagccggaa tgttatctgg catttttagc agagcctgaa tgccataatc
      481 acggctcccg gcgttggccg tcagtgggcg acactggcgg ctttttgttt tcctttactt
      541 tcattttctg tcggcggtga cggagacata catcagatgg aaaaaatcac aacaggtgtg
      601 tcatacacca cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc
      661 tctccgtccc agtgggtggc aatcggtgtg ctggggagtc tgctgtttgg cctgctgacg
      721 tatctgacta acctgtattt caaaatcaga gaggaccgtc gtaaggcggc gcggggggag
      781 taaagcgatg aagaaaaaat acgaactggg tgttaaaggg ataaataatt acccggataa
      841 gattactgtt actgtggcac tggaaattgg tgggtatccg tcactgttgt tgccagatgt
      901 ggcgattagt cttgaccgta ctgaaggtgc cacgctggag ttttacgaag ctgaggcgaa
      961 aaagcaggcg aagcagtttt tcatggatgt tgctgccggg ttatgtgaag gggatggtcc
     1021 gttgccggaa aagcgccccg taattttaga ggcgcaggat gtgttgataa cctacagagg
     1081 aaaactaccg ggaataatta ctggttctct gaagactcca ccgctggcct gaagacttaa
     1141 catatccagg gatttgaaat cgataaaccc tgataaatat ccatgaacgc aaaaatcaga
     1201 tacggcctgt cggctgccgt tctggcgctg attggtgcag gggcgtctgc gcctgaaatc
     1261 ctcgaccagt ttcttgacga aaaagaaggt aaccacacca cggcataccg tgatggtgcg
     1321 gggatctgga ccatctgccg cggtaccacc cgagtggatg gtaaacctgt cgtcccgggc
     1381 atgaagttgt cgaaggaaaa atgcgaccag gttaacgcca ttgaacgtga taaggcgctg
     1441 gagtgggtgg agcgcaatat taaagtaccg ctgaccgaac cccagaaggc gggtatcgcg
     1501 tcattttgtc cttataacat tggccccggt aagtgtttcc cgtctacgtt ttacagacgg
     1561 attaatgcag gtgatctaaa aggtgcctgc gaagcgattc gctggtggat taaggacggt
     1621 ggcagagact gccgtattcg ctcaaacaac tgctacggtc aggtttcacg gcgtgaccag
     1681 gagagcgcgc tggcgtgctg gggaattgac agataagcag aatattttgc tgaaaaatgc
     1741 ggtttgctca cactggcgga taacacgaaa tcctgcgaac tggcaaaaac taagtgaata
     1801 aaagtaaaac cccgtttgtt ggccgcaaac ggggttttgt gtttctgacc ttgagtaagg
     1861 caagggagaa catgggaaag tataaacgaa ttctgttgag attgactatg aaaaacggcc
     1921 ttgaactgaa agcgcctgta actgatgacg tcagcagagc gctggctttt gctattaagt
     1981 gggtggcggt cggtattgct gtgtctccga tgctgtatgg gctggcaaaa ctggtcattg
     2041 cgttgaaatc gtgaagagga ttaagcatgt cagacaagct cataacgctg gcgaagatcc
     2101 tctgtgtaat cgtcggcatt tcattttcac taatgctggt tgctatttgc ttttccactg
     2161 cctggcgcgt cttaactttg tcatggctgg tggggtgagg gggatatgaa ccgtgttctg
     2221 tgtgtggtga ttattgtcct gctggtagcc tgtggtgtgc ttagtctggg gctgaatcat
     2281 taccgcgata acgccattac ctacaaagcg caacgcgata aaaaagccag tgagctgaag
     2341 ctggcgaacg tgacaattac tgatatgcag gtacgccagc gtgatgt
//
LOCUS       DAZFWP020000318         2378 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFWP020000318 DAZFWP020000000
VERSION     DAZFWP020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.320"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..235
                     /locus_tag="ACHK0Z_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /pseudo
     CDS             1..235
                     /locus_tag="ACHK0Z_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(316..474)
                     /locus_tag="ACHK0Z_RS25590"
                     /old_locus_tag="pgaptmp_005118"
     CDS             complement(316..474)
                     /locus_tag="ACHK0Z_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4504957.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(560..1303)
                     /locus_tag="ACHK0Z_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             complement(560..1303)
                     /locus_tag="ACHK0Z_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504958.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(1488..2177)
                     /locus_tag="ACHK0Z_RS25600"
                     /old_locus_tag="pgaptmp_005120"
     CDS             complement(1488..2177)
                     /locus_tag="ACHK0Z_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4504959.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(2192..2314)
                     /locus_tag="ACHK0Z_RS25605"
                     /old_locus_tag="pgaptmp_005121"
     CDS             complement(2192..2314)
                     /locus_tag="ACHK0Z_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU4504960.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          787 a          577 c          403 g          611 t
ORIGIN      
        1 taatgtttaa tcgacatcga atttctcccg tgtagaggaa cagagttaaa aagccggaag
       61 cggaatcaaa tcacaggatg accatctgcc agtggcaggt cataaaaaaa aggctgcgca
      121 atgcgcagcc agaactcaca aggaaaatga taaaaggaat aacactagtg atgtacgcat
      181 ggcgcctccc gctaagttct gcaatgatca aacagaactc gctacgtgcc cttaaaactc
      241 gatcatttag cccctccaag gaggattcac catgcggttg attttttaat aaacagtaaa
      301 caaaaaagtc aagaattatt cattctgttc tttcatcatc ggccacagca ataccacaat
      361 gccgcagacc agagcgccat cagtcagtac caacattatc ctgctggtga aatccatcat
      421 caccatcact aaaagcagga tcacaacagc aagcagacac agtttataaa acaatgttca
      481 gaaaacgcat tcagcatgcc taaggttcta ttcctacgaa tagccaactt gcaacttaaa
      541 atattattta tgcagccaat taaattctgg tccttacaat atcaacctga agattcttat
      601 cttgtgctga ttgataaatg acaaaccttt tactacctgc attgaaagaa gtagacaaaa
      661 ccagacaatt atcataacga gcaagaacat aataccaacc atcattataa ttaatcattt
      721 catattcttt cttaaactgt ggtttgtaat atcctgtcag aaatgaaaaa agccaaaaat
      781 atgccacaaa agcaatcatc acaatctcaa aaaaatgttt ttttataaat ggcttatcat
      841 agaagcatga taccgataaa aatcgcccat aagatcttat cgaaattgta accgccagcg
      901 caatcgctgc tgacagtagc aaaagaggta cctgaatctt ctgtctcaat atagaaaact
      961 caataattgc cggcacaaac aataattcca cagcaaaata aaggcgaaat acatttagct
     1021 cttgcataga atgttttctt ttcactgcga aaaagaatac aacaccaata ccccaaccga
     1081 taagaaatat agcaatgacg ataactgcaa aaaataaact tctggcaaca tcatcaacac
     1141 ctgcacctac aatccaccat gggaagccgt agtaaaaaga agtaccccat ccatagaaat
     1201 aagcactccc ccatccaagg catcccatgt aggcaataaa aagtgaagaa ctcctgagca
     1261 gcgcaccatc cttcataacc accccaatac aagatgataa cattggctta caactcataa
     1321 caaaagcaat tcaatgccgt caagaggtta caggctaaaa aaactctatt acatagcagc
     1381 cagcatgttt accgtacaag tacaactcag ggcataaaaa aaacccactc ggcagcgggt
     1441 ttatacattt tttacaacat accaaatttg catgaagcat atggctttta atccagtttt
     1501 gcaatatttt gctgtaaaaa tgctgccttt tgttttgaac gtgttctcgt cacaagcaat
     1561 aaagcatcac tatcaagctg tagaaaaatg tgcttcattg caacccagcg ttcagtaaat
     1621 gtctcggacc agttttttgt tgtcactccc accaatgatg ccagtgtctg gtattcatag
     1681 gcctcacgcc ctgcaagttc gctcttcaca tcctgtgcag ccagccagat taacgtcttc
     1741 aggcgatcca gtgtcttacc tgcaattttt ctgttactta acaaatcttt aaactcgctc
     1801 catgcccatt gcgttatggt gacctgatgc ccccatcgaa cgctttcgct gtaacaccaa
     1861 agcaaccatg ctttctgatg ttcatcgaga gacaaaaccg cgcggcgcca tgaagaggtt
     1921 gagaattcaa ccgggctgac caaagcaatg gatgaacctt ttgcgtacga ctgcttaccg
     1981 gaagtcggcg tattatccag cgtaatcatc ttgccagtta ccacatccag aatgcgcggc
     2041 ttctttcgtt tgtatgtacc agtatcaaat tgtgcatgct cctgccaggc ttcgagctgg
     2101 cctttcgttg ctccgttcaa gtcagcagta gctgccataa gttgctcacg aacatactgt
     2161 aaatattggg tattcatgca gtaaatcctt tctatatttt ggcataattc ttcaacattc
     2221 ggtaatcgtt caaaaccgaa tcggggaaac gacataagca caggagcccc cagcgacagc
     2281 gaaggagttc tgatatataa gactcagaca tcattcattc cccggttctc caatatctgt
     2341 ttcactcatc atccataact acctgtaatt gcccccct
//
LOCUS       DAZFWP020000319         2359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFWP020000319 DAZFWP020000000
VERSION     DAZFWP020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.321"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2262
                     /locus_tag="ACHK0Z_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             <1..2262
                     /locus_tag="ACHK0Z_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4504961.1"
                     /translation="LTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSE
                     DEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDK
                     GRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGM
                     KLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRA
                     YGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPE
                     LHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQ
                     HRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEMTWYGWD
                     GDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHG
                     VVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFY
                     HCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYY
                     NRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDS
                     YGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAI
                     TYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVM
                     TLACRTWQECWVN"
BASE COUNT          578 a          595 c          743 g          443 t
ORIGIN      
        1 ctgactaacg agaacggcag ccacagcgac ttcagctacg atgcgctgga ccggttggta
       61 cagcagggcg gctttgacgg gcggacgcaa cgttatcatt atgacctgac cggaaaactc
      121 acgcagagtg aagatgaggg gcttgtcacc ctctggtact acgatgaatc ggaccgtatc
      181 actcaccgca cggtgaacgg cgaaccggca gagcagtggc agtatgatga ccacggctgg
      241 ctgacagaca tcagccacct gagcgaaggc caccgtgtcg ctgtccacta cggctacgac
      301 gataagggcc gcctgaccgg cgaacggcag acggtggaga acccggagac gggggaactg
      361 ctgtggcagc atgagacaac acacgcctac aacgaacagg ggctggcaaa ccgcgtcacg
      421 ccggacagcc tgccgccggt ggagtggctg acctacggca gcggttatct ggcgggcatg
      481 aagcttggcg acacgccgct gctggagtac acgcgcgacc gcatgcaccg ggagacggtg
      541 cgcagctttg gcagcatggc aggcagtaat gccgcatata aactgaccag cacatacacc
      601 cccgcaggcc agttacagag ccagcacctg aacagcctgg tgtatgaccg tgcctacggg
      661 tggaatgaca acggcgacct ggtgcgcatc agcggcccgc gacagacgcg ggaatacggc
      721 tacagcgcca cgggcaggct ggagagcgtg cgtaccctcg caccggacct ggacatccgc
      781 atcccgtatg ccaccgaccc ggcgggaaac cgcctgccgg acccggagct gcacccggac
      841 agtaccctca cggcgtggcc ggataaccgc atcgcagagg atgcgcacta tgtttaccac
      901 tatgatgaat acggcagact gacggagaag acggacctta tcccggcggg tgttatccgg
      961 acggacgacg agcggaccca ccactaccac tacgacagcc agcaccgtct ggtgttctac
     1021 acgcggatac agcatggcga gccactggtc gagagccgct acctctacga cccgctggga
     1081 cggcgaatgg cgaaacgggt atggcggcgg gagcgtgacc tgacggggtg gatgtcgctg
     1141 tcgcgtaaac cggagatgac gtggtacggg tgggacggcg accggctgac gacggtacag
     1201 accgacacca cgcgtatcca gacggtatat cagccgggga gcttcgcgcc gctcatccgc
     1261 atcgaaacag acaacggcga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
     1321 cagcaggaag ggagcgagga cgggcacggc gtggtatttc cggctgaact ggtgcggctg
     1381 ctggacaggc tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
     1441 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
     1501 ccggcgcgaa aagttcattt ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
     1561 agcgaagacg gcaatacggc gtggcgcggg gagtatgatg aatggggcaa ccagcttaat
     1621 gaagagaacc cgtattacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
     1681 gaatcagggc tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
     1741 acacaagacc ccattgggct ggcgggggga tggaacctgt ataattaccc actgaatccg
     1801 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
     1861 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
     1921 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
     1981 gatatttgta tttatgctac agcatgcgga catgcaggaa ttggtggtgg gataaatgcg
     2041 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagcaa ttcagtaggt
     2101 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
     2161 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
     2221 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
     2281 aatatatatt tttagtttta tttttattgt gtggttgttc tccagtggac atagagccag
     2341 ctagacaaga agttaatat
//
LOCUS       DAZFWP020000320         2345 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFWP020000320 DAZFWP020000000
VERSION     DAZFWP020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.322"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..348)
                     /locus_tag="ACHK0Z_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     CDS             complement(<1..348)
                     /locus_tag="ACHK0Z_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4504962.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAG"
     gene            complement(329..742)
                     /locus_tag="ACHK0Z_RS25620"
                     /old_locus_tag="pgaptmp_005124"
     CDS             complement(329..742)
                     /locus_tag="ACHK0Z_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4504963.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
     gene            complement(769..1200)
                     /locus_tag="ACHK0Z_RS25625"
                     /old_locus_tag="pgaptmp_005125"
     CDS             complement(769..1200)
                     /locus_tag="ACHK0Z_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4504964.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(1214..1966)
                     /locus_tag="ACHK0Z_RS25630"
                     /old_locus_tag="pgaptmp_005126"
     CDS             complement(1214..1966)
                     /locus_tag="ACHK0Z_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4504965.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            complement(1974..>2345)
                     /locus_tag="ACHK0Z_RS25635"
                     /old_locus_tag="pgaptmp_005127"
     CDS             complement(1974..>2345)
                     /locus_tag="ACHK0Z_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4504966.1"
                     /translation="AVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLTDAEYTGEE
                     LDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTMVQQGYDYR
                     RDDDMALWSSADLTYSITYEM"
BASE COUNT          529 a          741 c          548 g          527 t
ORIGIN      
        1 accggcaagc cccctgaaca tggggatcat cccgccgaag gagtccttca cctgaccgcc
       61 ctgttgcagc aggatcagcc agggattctg accaccggca agctgcgtgg cgatatccgt
      121 aaactgtgcg ggcagggttc gcatggccgc tttatactgc ccgacggaaa tcccggcttt
      181 ttgtgcagcc agcgcctggc ggctcaggcc ctgttcaaca gcactggcgg tttttctggc
      241 gtcggtatcc agacctgaaa aatgacgcct tacccggctc atctgctcat cgaaacggac
      301 cgcatccaga ctaaggtcaa taacaagatc accaaccggc tgggacatat ctcacacctc
      361 cggaaatccc cgctgaagcc atcattaatg cggcatcatc caccatgaca tccgccacat
      421 ccgcagacga taaaatatcg cgccctccgt ccccaccgaa ccggacgcct ccggcaagtc
      481 ctgccgcttt ctgcatcagc attttgtcct catccggcct ctccacctgc tcttcctcat
      541 gccgggggac aagcagactg aaatcagagg gatgcatatc cggatcgcaa aaaaacaggc
      601 tgagtacagc gtacgtcagc ccggaaaaat gcatatccag ctgggtatcg tgaaaataat
      661 gcatgcggta aaaatgtcgc cagtcggcat attcggtgga tgtcatcccg gcaagcatgg
      721 cgcgccagtc aggcctcccc atctcacgcg ccagtctgag ggcaaaattc agctcgccgt
      781 cgaagacttt cccgcagaaa aatcatcatc agtcagcgcg ttatttttcg ccacttcggt
      841 aatatccgta tccacatgaa caggcccgct catcccggac agacgcaaca ccacatcttc
      901 cgcccgggca atggcatcag caggccaggt ggtcaggact tcctgctcaa tctgcatcac
      961 agcctcattc attgacggtg accccgtttt ctgcggatgg ttatgccaca gggacatcgc
     1021 caccagaaac gcgccggttc tgacgagatc ttccacgctt acctgcaggt tgccgctgga
     1081 ttctgcctgt tctgcacgcc gtttcaggag ggcaagatgc tcaatacgct gcagcgcaga
     1141 caattcggaa agcgtgacag acacaccgtt atattcaaat tgttctgttt tcagaaacat
     1201 gtatgacctc cgtttaccct gcagcgcctg cttcagtaac ggtgacttca gctaccgtgg
     1261 caaactgacc attaccggaa atcacgggga tactcacttt tccagcctta acccccgtca
     1321 cagtgatcac catatctttc acagcaatgg ttcccgttga cggatcggcg gaaaccgctc
     1381 tgaacgtctt gtcggttgca ctttccggct caaaagaaac agtcagggtg gttgtttttc
     1441 ctttttccac ggtaccggat gtcggtgtca ccttaattgc agtggccggc gtaatttcgc
     1501 tgcgttcttc cgccacggaa ggtttaccca cgttggtcac tttcaccgta cgggtgatca
     1561 cttccttcgc cgttacggcc ttaccgatac tgctgaccca gccacggaac acatccaccg
     1621 tgccgttcgg gaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     1681 gccctttctg accttcttct cccggtttcc aggccagcgt aaaactggta tctcctgcag
     1741 acttctgccc ctgcccggtc gcgctccagt ccgcgtcttc atcatccagg tagttatcat
     1801 cgtaggattc tgccgtcatc tcgcccggcg tcagatcctt caccttagcc agtcgctgcc
     1861 agtcatcatc tgacaacggg tttgcataag catcaccctt gccggtgtaa acccacagag
     1921 tggtaccggc accttttacc ggcgccaggg gatttggtgt tggcatatcg tcctcacatc
     1981 tcgtatgtaa tggaataagt caaatctgca gagctccata acgccatatc gtcatcacga
     2041 cgatagtcat agccctgctg aaccatcgtg gtaatcatcc ctgccagtgc agggatcgcg
     2101 gacatcgccg gataaatccg ggactccatc cacgaatcca gctctgaatc cggcacctga
     2161 gcaggcagga aaacttcaat atgcagtgtg gcccgccagg tatccgcatc cagctcttca
     2221 ccggtatact ctgcatccgt cagataaacc gcgaccgcgg gaaaatcctc ttcgtcaaaa
     2281 acaacggggc gaccatcaaa cagcgtcgcc ccgtgttcat gcagctccag tgcatccagc
     2341 actgc
//
LOCUS       DAZFWP020000321         2319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFWP020000321 DAZFWP020000000
VERSION     DAZFWP020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.323"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..681
                     /locus_tag="ACHK0Z_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             <1..681
                     /locus_tag="ACHK0Z_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4504967.1"
                     /translation="TPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNV
                     GRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRR
                     GLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKL
                     NVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDVEKAITD
                     FMTARGYH"
     gene            complement(730..2229)
                     /gene="pitB"
                     /locus_tag="ACHK0Z_RS25645"
                     /old_locus_tag="pgaptmp_005129"
     CDS             complement(730..2229)
                     /gene="pitB"
                     /locus_tag="ACHK0Z_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGU4504968.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLTV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          616 a          595 c          568 g          540 t
ORIGIN      
        1 acacctccat taattgccgt tattggtagc gatggttcag gcaagtcaac ggtgtgtgaa
       61 catcttatta ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacag
      121 gccggaaatg tcggtcgtgc agtgacaaaa ttaccgttga tgggaaaatc cttacataaa
      181 acaattgaac gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt tccggcgctg
      241 gtaattacag cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct tgcctgtcgt
      301 cgtcgcgggt taattgttct aaccgaccgt tatcctcagg accaaattcc tggcgcttac
      361 gatggtacgg tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg gctggcaagc
      421 caggaacgta aagcgtttca ctggatggcg agccataagc ctgatctggt catcaaactc
      481 aatgttgacc ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga atcgctggcg
      541 aggaagattg ccataacgcc acagttaacc tttggtggtg cacaactggt tgatatcgat
      601 gccaatcagc cactggaaca ggtgttggtt gatgtagaaa aagcgattac ggattttatg
      661 accgcgcgtg gttatcacta gtcaaaaatg gaaatgcccg atcgccagga ccgggcattt
      721 tcaggaaggt taaatcaact gcaatgctat ccagtacagc ccaccagaaa gaaaaattgc
      781 cgccggtaaa gtaaacaccc acgccatcag gatgctggtt accgttttac gctgtaaccc
      841 accgccgtcc accaccatcg tccctgctac cgcagacgag aggacgtgtg ttgtggagac
      901 aggcatccca atataactgg caaggccgat agacactgcc gccgtcattt gtgccgccat
      961 gccttgcgca tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg
     1021 acgccagcca atcatggtgc caatgccgag cgccagtgct accgccatga tgatccacac
     1081 cggagcgtac tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc
     1141 ttctttactg acgcctggca gtttcgccag cttcgcggag gtatcagaga tgcacagcat
     1201 aatgcggcgc agctggctgc gctgactcac ggttaacggc tcgtaacttt ccatattgcc
     1261 tggcagcatc gttttaacgc gcgcaatagc atcaaaggta tttgccggat gacagtgaaa
     1321 ctctgttact tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca tcgcaatcaa
     1381 cttctgcggc agttcaggat gctgttgcag gtagtgttcg aagttggtaa cggcatcgcg
     1441 ggtacgggta atttcatagc cggacgcatt catattgacg acgaagccag caggggcaat
     1501 ccccaccagt accagcatta ccaggccgat ccctttctgt ccgtcgttcg cgccgtgcga
     1561 aaacgccacg cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg gcggtttacg
     1621 tttgcctttt ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt
     1681 cccgctccag tagcgtcgca gcaggaatat caggcctccc gcaatgacca ggccgacgat
     1741 aggggaaaca atcagcgagg agaaaatttt ggtcacttca cgcaggttta acgcatccat
     1801 caccgatgag ccggttaaca gcgcgttggt taaaccgatg ccgataatcg caccaatcaa
     1861 ggtgtgcgaa ctggaggccg gtaaaccgaa gaaccacgtt cccaggttcc agataatcgc
     1921 cgccagcagc atggaaaaga ccatcgccag gccgtgggtt gaccccatat tcagcaacaa
     1981 atcggttggc aacatatgga caatggcata ggcaacgcta agtccgccca ataacacgcc
     2041 aaaaaagtta aaaaatgccg ccatcaccac agcaagttgt ggttgcatgg cacgagtata
     2101 aataacggtt gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa
     2161 cacaaatgcc agagcaagca ataacaaaag ccctgtgtat atatcaaggc caacaaataa
     2221 atttagcata tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact
     2281 ttaacggcat gggcaaagtg aaatatcatt ttttaacga
//
LOCUS       DAZFWP020000322         2267 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFWP020000322 DAZFWP020000000
VERSION     DAZFWP020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.324"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..133
                     /locus_tag="ACHK0Z_RS25650"
                     /old_locus_tag="pgaptmp_005130"
     CDS             <1..133
                     /locus_tag="ACHK0Z_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HGU4504969.1"
                     /translation="RNEFNPIDISSYNVSDISWSRHEIMESYHGGKQDLISVVLSKI"
     gene            350..1187
                     /gene="cif"
                     /locus_tag="ACHK0Z_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /pseudo
     CDS             350..1187
                     /gene="cif"
                     /locus_tag="ACHK0Z_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428038.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
     gene            complement(1323..1577)
                     /locus_tag="ACHK0Z_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /pseudo
     CDS             complement(1323..1577)
                     /locus_tag="ACHK0Z_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1734..>2267
                     /locus_tag="ACHK0Z_RS25665"
                     /old_locus_tag="pgaptmp_005133"
     CDS             1734..>2267
                     /locus_tag="ACHK0Z_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4504970.1"
                     /translation="MPLTSGIGSHSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TDGSFSSSGGNGGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQEGIQSKISQYK
                     FSVCPERLQCPPESAQCPITLEIPEEGVFIKNSGDSVVCSLFDVTAFSRLVSEKSPHP
                     LTREKLTASMVVSADKCF"
BASE COUNT          689 a          391 c          514 g          673 t
ORIGIN      
        1 gagaaatgaa tttaatccaa tagatatatc atcttataat gtttctgata tttcatggag
       61 tagacatgaa atcatggaat cttatcatgg aggaaagcaa gatcttatta gtgtggtatt
      121 aagtaaaatt tagtaatttt atccagtgta gtggatttgt tgcatggatg gagttggtaa
      181 aaagtggtgt ggctggcaat ccaggccgcg tcacagaaat ggacaatgcc gctgagagac
      241 tggcgaatgg catacaggca ccagaaaaat ttcagacatg acataatgcc catgccagaa
      301 gttccttttg cacctcgcct gtatttatag ataaacatag agtgaatgaa tgagatatga
      361 aagacattac ccttcccccc ccgacgtccg cgtcctgtct gacaggggcc atatctgtaa
      421 atactgaagc tgtattatct cccatgcaac acacttcagc cttacatgta agagattttg
      481 cttccctgtg ctcacagaac ctcaaagcta atgtattgct aaatagtgat gaccacgaag
      541 tacctataca tcagaaaaat cctgctgcaa taatgcaaaa tatcgactct aacatcaaac
      601 agatggcaac agactggggg atgtcgattg aggaggttga ggttattata gggcgagaga
      661 aaggcattgt ggaaccctcc tgcggagtta ccgctaatgc tattatgaaa ctatttctgg
      721 acaaggatgg cttcagttac tgctttgaaa atgaacagac actatcgctc gagcagcttc
      781 aggaacgcct gtcctgtatg cctgaatgta agagctttgt attacgtgtt aatgatggtg
      841 cgcttggtca tgcttacatt gtcgatattc ccaaaggaga aaactcttgt cgtcctgcat
      901 tcttgtatca gtcagattta ggagagggcg tcaccagaaa gttaagattt gaggactgga
      961 tgacgcataa agcattgact ccgattttgc tggatgatat ttgtaattac ttctcctgca
     1021 tgtctcaaaa taagacagat ttggagcaga ttgcaacgtt atttgatatt gatgggaatg
     1081 ttaaaatgtt acgaaaggaa aatattcaat atcaaaagca tgacaatttt agtttccagt
     1141 tgtttgagta tgacaccgat aatattgaaa aaaaaacatt gagataataa aatcactatg
     1201 tagttagctt ttgttaaaat tgcaattatt tgtaaacata ataataagta ttatgacgtt
     1261 aaaatttaac catacggtaa aatgattcag gaaaaccggt tcagcatagc ctaggctgaa
     1321 cttttgagga tatcaacgaa cggaattttt gaacatttat gagtagatcg ttgttgaagg
     1381 gcttcaatga gcatgtcaag ttcatcaact gctggtctac gattacgacg gtttgattta
     1441 ccaatcaaag caagtttaag tagatatgga cgagcacttt tcgccgggtt tgatgtgtaa
     1501 ttaattccgt atataggttt ggctgcatcc agaacactgc caagataact aacatcgtga
     1561 ctgactgttg ctggacctac aaatgccaat cagacggaat gttgaaagga tagaatgaac
     1621 agcccgacac acaaaaaacg gagtccggct ccggtttttg tgttgccata taaggcagat
     1681 gtttgttaca gctatttaag tctggagttc aaattaaaat agggagtttt gttatgccat
     1741 taacctcagg tattggatca cattcattta atcttgggct ggaggttttt cgtgcccgaa
     1801 ttgcagccaa tgggcgcgga gatattacag taggtggtga aactgtcagt attgtgtatg
     1861 atgctactga tgggagcttt tcatccagtg gcggtaatgg cggattgctt tctgagttat
     1921 tgattttggg atttaataat ggtcctcgag cccttagtga gagaatgcta agtatgcttt
     1981 cggactcagg tgaagcacaa tcgcaagagg gtattcagag caaaatatct caatataagt
     2041 tttctgtttg tccagagaga cttcagtgcc cgccagagtc tgctcaatgt cccattacat
     2101 tggagatacc agaggaaggg gtgtttatta agaattcagg ggattcagtt gtatgtagtt
     2161 tatttgatgt tactgcattt tctcggttag ttagtgaaaa atcacctcac ccactgacac
     2221 gagagaaact aacggcatca atggttgtaa gtgctgataa atgtttt
//
LOCUS       DAZFWP020000323         2229 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFWP020000323 DAZFWP020000000
VERSION     DAZFWP020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.325"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..404)
                     /locus_tag="ACHK0Z_RS25670"
                     /old_locus_tag="pgaptmp_005134"
     CDS             complement(<1..404)
                     /locus_tag="ACHK0Z_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4504971.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEV"
     gene            complement(465..1112)
                     /locus_tag="ACHK0Z_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             complement(465..1112)
                     /locus_tag="ACHK0Z_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4504972.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPVFRQKLNEGWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(1010..1753)
                     /locus_tag="ACHK0Z_RS25680"
                     /old_locus_tag="pgaptmp_005136"
     CDS             complement(1010..1753)
                     /locus_tag="ACHK0Z_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4504973.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLVAVSTFV"
     gene            complement(1759..>2229)
                     /locus_tag="ACHK0Z_RS25685"
                     /old_locus_tag="pgaptmp_005137"
     CDS             complement(1759..>2229)
                     /locus_tag="ACHK0Z_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4504974.1"
                     /translation="PTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKVYARFLDAVNFVN
                     GNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYR
                     GDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          468 a          748 c          557 g          456 t
ORIGIN      
        1 acttccgacg gattccggtc cccctttgag gtggtttcca ccagtgcctg cacaccgaag
       61 gtaaagcgca gtcggtcgat gtttgccgac gtgatggcgc gggtgatcgg cgtgtcatat
      121 ttcacttctg taccgagcac cgtctcggag ccggaggatt caaatccctc cggcggtgtc
      181 tgctcctgct caccggcccg gaacaccacc gtgacgccgg atatattggt attcccctca
      241 ctgtccagca ccggcgtact gttcagcagc acgcttttta atccatccac cggaccttca
      301 accggccctt cgctgatggc atcgatcaca ctcagcagct gcgtggactt caggttgtcc
      361 ttcgcttcgc gcggggtatg ccccttactg ctgcctttac ccattcctca cgctccataa
      421 acgataaaac cgcccggagg cggtttcaca taaaacattt tgcatcagcg accaatcacc
      481 acaacctgac caccatcccc ttcgtctgcc gtgctgatct cctgagaaac cacacgtgac
      541 cccacgcgca tttcaccgta cagaacaggc agaacattgc cctgggcaac catgttatcc
      601 agtgaggaga aataggtgtt ctgtttgccg ttatctgtac tggctgccgt gggcgtcctg
      661 gctttcggtg ccagcatctg cgccactccg cccaggatca tactggcccc tgccgcatac
      721 atgcccgata cagccgcggc ccccagccag cccacagggt tccaccatgc caccgcaatc
      781 agcgccgccc caagcaccac ctgaaacaca ccgccacttt tagctcccgc cagacgcggc
      841 acgatgtgga tcacggcacc atttgccagc ggctcattaa gacgggcaga taattcattt
      901 tcgcctgcat cacgcccggc aatgcgcacc tgataccagc cctcattcag tttctgacga
      961 aacaccggga gctgtgtggc cagtgcccgg atggcttcag cccccgtttt cacacgaagg
     1021 tcgatacggc gaccaaatcg ttgcaaatcc ccgtaaaggc agatgcgtgc catgcccggt
     1081 gacgccagag ggagtgtgtg cgtcgctgcc atttgtcggt gtacctctct cgtttactca
     1141 gttgttcagg aatatggtgc agcagctcgc cgtcgccaca gtaaatggcg gcatgattcg
     1201 gcaccgatga accaaaacag cacagcagca catcgcccgg ctgggccgct gacaacggca
     1261 cctgatacaa ccccgttgcc tccatattat ccagatagag attctggccg tgacgccacc
     1321 agtcatcccc gcgatgaaaa tccggcatct caattcccgc cagatggtaa gcgtcccgga
     1381 acagcgtgta acagtccgtc accccgtgct caaagcgccg cccggtgaga tgcggcacac
     1441 agcggaattt atgaatcgcc ccccggcaga ccagccacca cggcaaatca ctctgcacct
     1501 gcagccgccg gtcggcctca ctcagccagg gcagaccacc ggggtggctg tggaccagcg
     1561 ccacaatctc tccctgcatt tctgcactca gccagtcctc cggcgacatc cggaaatacg
     1621 cctccggctc accagagata ttcacgcagg gaaaatatct ttccccctcc ggtgttctca
     1681 ccacgaagcc gcacgactcc gctggcgcac atcgccgggc gtgcgccaga atcgctgatt
     1741 ctgtctctgt catgggattc actgcgaaag tttgttaatg gaaaggaagc cgccaaagtt
     1801 gccgacgtta ttgcgaaact tacagccgct caggcatttg ctgcatttat ccttcgtgat
     1861 atcggacgtc ggctgatcat attcatccgc gaccgccgga ccgtgataac cgcactcgtc
     1921 accgcgatag gtccaggtgc aggtgttggc cagcatgatg cgccccggaa aaacggcacc
     1981 gtccgtttcc gtcggcgtgg acagtacaaa agaggcactc accgcgctca gttcgctgca
     2041 ctgctcgatg cgccagcggc tgatcacctc ctgctccgga tcggcgtaac tgtttccgtt
     2101 gacgaagttc accgcatcca gaaaacgggc gtaaacctta cgccggacca ccgttccgcc
     2161 gaccagactc tgcatatctt ccgccatccc ggtgaccata ccgtacaggt tagaaaccgt
     2221 cagcgtggg
//
LOCUS       DAZFWP020000324         2224 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFWP020000324 DAZFWP020000000
VERSION     DAZFWP020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.326"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..275)
                     /locus_tag="ACHK0Z_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     CDS             complement(<1..275)
                     /locus_tag="ACHK0Z_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4504975.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQITRYSDTRWHEDSV"
     gene            complement(346..>2224)
                     /locus_tag="ACHK0Z_RS25695"
                     /old_locus_tag="pgaptmp_005139"
     CDS             complement(346..>2224)
                     /locus_tag="ACHK0Z_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4504976.1"
                     /translation="PDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWD
                     TPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQ
                     QGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQ
                     VETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLD
                     FFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQ
                     QIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAM
                     INPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADI
                     SGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNG
                     TVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIAS
                     RETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISI
                     R"
BASE COUNT          473 a          640 c          545 g          566 t
ORIGIN      
        1 acggaatctt catgccagcg ggtatcgctg taacgggtaa tctggcgatt cctgtctcct
       61 gcatagctga atgacgtcac cagccccagc gtgtccgtga attcataacg gtatttcacg
      121 ttaatcccgt taagattatc gctaccggga gcgttcgtcc gggcatgaag ataccccgcg
      181 ctcagcgtgg actgatgttc agacgcccat gcaggcgcac cggatacggc cagacagatg
      241 gctgcggaca aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata
      301 aaaaaggcgt cattcctgac gccctttatt ggggttataa atatttcaac gaatactgat
      361 gccggaagca gcttttttgg tcacaatcac cgtacagtcg gtgatattgc ctgccccctg
      421 attgcctttc tggaaaatct taaactccag agtgacgcta ccaccaccac taggcatatc
      481 aataactgca ctgtaactac cgggaatggc ccctttagtt tctctggatg cgattaatac
      541 gccgtttttg cgaacttcaa aaccataccc cgtgtatcgc gtgcctcctg ggttatttcc
      601 gctccctgga tcgtcatacg ctataccgtt aaaaataatg ggcggaataa taatctggcg
      661 gtcaaagtta tgatcatcgc tgatggtgac tgtaaccgta ccgtttggtg tttccgtgtt
      721 accccacgta ccgacttttt tcgggaaggc ttttgataca gctttaacga aatcccctct
      781 gacctgggtc gcctccagca tgcccttaat cgtacagttc tggttaatcg tgacattgtt
      841 gagcgctcct gagttcgcat tcacactgcc actgatatcc gcatttttcg ccgtcagtcg
      901 cccgtctggt gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt
      961 cagatatttc aggaacactt cattcatgaa tatctgatcg ccctgaccaa caaacatcgg
     1021 ctttgtgttg ccattcgcag gattaatcat cgcaatcctg tctgctgcca gcagcacctg
     1081 actctgcatg ccgtcggggg tgttctcaat accggcaccg atacccgcaa tataaaggcg
     1141 tccgtcctgc atctgctgca gcttcactgc ccacatgctg ttcaggttat tatttgtatc
     1201 aacctgaacc ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac
     1261 ggtctgtgtt atttcattgc tgacatccgt tatggacgtc ctgatttcag tcaggtcagg
     1321 cgcaagctga ccgttatcaa tctgcgtcca cagctcctga gccagatggg ttttccctat
     1381 ctcgcctttg aaaaaatcca gatagcctga tgcatcatca ctcggctgac cgacagcctc
     1441 tacaaatgcc gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc
     1501 cggtttgata ttgatactgg cagctatcca gtacagcgcc gtgccaagat agcgggctgt
     1561 ggtttcaacc tgcctgatat cagcaatccg cttttccgag aaccagaact caaactgtac
     1621 cgtcggatca taaaccgcaa gatgcggcgt ggcggttatc tgaaaatagc ccggtgtcag
     1681 ctcaatccga gacggcgctg ccggtgcggc aatccggaac gataccgatg ccggatcgcc
     1741 ctgctgtccc cacgcattta ccgcccggac tgtcaacctg tagttccccg gcgccagttg
     1801 cgtgaagcgg tatgtggttt ctgtcgtcct ggccgtgctg accagccgct cactgccgtc
     1861 atccgctgcc acggtcaggc gaagcaggaa gctcacgccc ttcaccacct tcggcgtgtc
     1921 ccagcgcgcc agcacctgat attccccgct gtctgcggtg acttcggcgg tcaggtgctg
     1981 caccgctggc ggcgtgacac cattcaccgt gccgctctgg tcgccgtcaa agtgcgcccc
     2041 gttatccacg atggcttctt tttccggcac gtgctgcacc gccgtgatgg caaaggtgcc
     2101 gtccgtgttt tcccggatgg agacacagcg gaacaggcga cgacgcagtg acggcaggga
     2161 gagtccccat acaccgtatg tctccacacc atcaggcagg gtgctgacct gtatccggtc
     2221 cggc
//
LOCUS       DAZFWP020000325         2216 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFWP020000325 DAZFWP020000000
VERSION     DAZFWP020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.327"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            91..423
                     /gene="hdeA"
                     /locus_tag="ACHK0Z_RS25700"
                     /old_locus_tag="pgaptmp_005140"
     CDS             91..423
                     /gene="hdeA"
                     /locus_tag="ACHK0Z_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU4504977.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            539..865
                     /gene="hdeB"
                     /locus_tag="ACHK0Z_RS25705"
                     /old_locus_tag="pgaptmp_005141"
     CDS             539..865
                     /gene="hdeB"
                     /locus_tag="ACHK0Z_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU4504978.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            929..1576
                     /locus_tag="ACHK0Z_RS25710"
                     /old_locus_tag="pgaptmp_005142"
     CDS             929..1576
                     /locus_tag="ACHK0Z_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU4504979.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1618..2148)
                     /locus_tag="ACHK0Z_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             complement(1618..2148)
                     /locus_tag="ACHK0Z_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4504980.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          658 a          433 c          501 g          624 t
ORIGIN      
        1 caacatgaca tatacagaaa accaggttat aacctcagtg tcgaaattga ttcgtgacgg
       61 ctctttcact ttatagttga ggatattacg atgaaaaaag tattaggcgt tattcttggt
      121 ggtctgcttc ttctgccagt tgtgagcaat gcagcggatg cgcaaaaagc agctgataac
      181 aaaaaaccgg tcaactcctg gacctgtgaa gatttcctgg ctgtggacga atccttccag
      241 ccaactgcag ttggttttgc tgaagcgctg aacaacaaag ataaaccaga agatgcggtt
      301 ttagatgttc agggtattgc aaccgtaacc ccagctatcg ttcaggcttg tactcaggat
      361 aaacaagcca actttaaaga taaagttaaa ggcgaatggg acaaaattaa gaaagatatg
      421 taattccggg aatgcgttac atcgtacttc cttgcatatt gaacaggccg gaatatcttc
      481 tttaaaagca gctattcctc ctgttcatat ataatctcta tattgaatgg gttacaaaat
      541 gaatatttca tctctccgta aagcgtttat ttttatgggc gctgtagcgg ctttgtcact
      601 ggtgaacgca caatctgcgc tggcagccaa tgaatccgct aaagatatga cctgccagga
      661 atttattgat ctgaatccaa aagcaatgac cccggttgca tggtggatgc tgcatgaaga
      721 aacagtatat aaaggtggcg ataccgttac tttaaatgaa accgatctca ctcaaattcc
      781 taaagtgatc gaatactgta agaaaaaccc gcagaaaaat ttgtatacct tcaaaaatca
      841 agcatctaat gacttgccga attaatgagg tacaagtaaa aaggagtagc aagttgagcc
      901 atcttgctgc tcctttttgc atttttatat gacagcagaa tttattatac gtcttatact
      961 cgcggcaatt gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca aaggagcagg
     1021 gttacgcaca catgtattaa ttggcatggg aagcgccctg tttatgattg tttcgaaata
     1081 tggttttgct gacgtgctgt ctttagatca cgtcggactc gaccccagcc gtattgctgc
     1141 tcaggtggtg acgggcgtcg ggtttatcgg tgcaggtaac attctcgttc gtaaccaaaa
     1201 tattgtaggt ctgacgacgg cagcggatat ctgggtgacc gccgccatag gtatggttat
     1261 tggcagcggt atgtacgaac tgggtattta tggttcagtg atgaccttgt tggtgctgga
     1321 agtcttccat caattaacct tccgcctgat gaacaaaaat taccatttac agctgacatt
     1381 agtgaacggt aatacggtat caatgctcga ctggtttaag cagcaaaaaa taaaaacgga
     1441 tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc gccattgata tccagcttca
     1501 tgccacaacg tcgatagaag acctgttgcg attattaaaa ggtatggcag gggtgaaagg
     1561 cgtttcaatt agctaatctg tgtggcagcg tagccagact caccgtaagc ctgaaattca
     1621 caccagataa tcaatatgct gatggcgtac cagctcattg atccgcttaa gattgaggat
     1681 catcatgata ttgtgtttat ggcagtagaa cgtttttagc gatattttca gtttctccag
     1741 aatatcttcc gttgtcattt gattcgcaat gcagctcaga acctgatgtt gcttgcgcga
     1801 aagtgacaca gccgcttcag gcacagagca gtaactgcca ttgatgagtg cgacaaaatc
     1861 aatgatagtg atagtccgcg gaacaaaggt caccggcact ttccccaggc agcgtttaat
     1921 attaaagggg gagaaaataa tcacatggac aggttttaat cgttcaattt gagtgagaaa
     1981 attagaatga aatacattat tcattaatgt atcaataatg acgaaggcat tctgatgcaa
     2041 cattacgtcg atctcttctt cgttctgtat atgaacgaca ttacctttac tcagaatgtt
     2101 tttcatcgcg gtgaagaaca tcgtatccct ggtaattata agaaacataa ccgactcctg
     2161 gtgcggacat taactttata atcagtttgc caaaggtaat taacaggaca gggctt
//
LOCUS       DAZFWP020000326         2213 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFWP020000326 DAZFWP020000000
VERSION     DAZFWP020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.328"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..105)
                     /locus_tag="ACHK0Z_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     CDS             complement(<1..105)
                     /locus_tag="ACHK0Z_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504981.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVF"
     gene            complement(89..>2213)
                     /locus_tag="ACHK0Z_RS25725"
                     /old_locus_tag="pgaptmp_005145"
     CDS             complement(89..>2213)
                     /locus_tag="ACHK0Z_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4504982.1"
                     /translation="WHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSHTSEGHRVSV
                     HYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYG
                     SGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLN
                     SLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAG
                     NRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTH
                     HYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPE
                     ETWYGWDGDRLTTVQTDTTRIQTVYQPGSFTPLIRVETENGEREKAQRRSLAEKLQQE
                     GSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLP
                     ERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYD
                     KESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTS
                     GGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTF
                     GSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQ
                     LFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          404 a          698 c          560 g          551 t
ORIGIN      
        1 aaatacgatg tcagaatttg aattgttatc aaacccaagg taaacaacaa ataaacaaag
       61 ggtaattaaa aaaatgacaa gtttgcctct aataatttga agcatctgaa ctcccttgct
      121 ttgcttttat tggattatct gcttttatac catctaaccc gaagagttgc ccagtgttat
      181 atttttcaat acatgcatcg ctacatttgg cagaaaaatc gccacaagca ataccgacag
      241 tgttattttt aagatccatg attctttctt tagctggctg accttgtcta tcccctgccg
      301 cctcatgatt tttacctatc acgtcagcta ttgttgatcc aaatgtctta gtcattcgac
      361 acatcaagta acaatgtctc attgcatcgg cggccccatt atgagctccg ccaacaatag
      421 catccatatc ataaccgaac tggtaagcac tgttagtaat gctgtttgca gcaatcaatc
      481 caggtacacc agatacggca taaattagtc cacctgatgt catattctca atcgcatcta
      541 accccattgg gtctatcact tgtatgggat tcaacggata ctgatacata ttccatccac
      601 ctctcaaccc aatcgggtcc ggggtgatat accgcccctg caacggatcg tagtaccggt
      661 gacggttata gtacagcccc gactccttat catactgctg ccccggcagc cggtacggct
      721 ggtgcaggtg atgcgggttc tcctcattaa gctggttgcc ccattcatca tattccgcgc
      781 tccacgccgt attgccgtct tcactgataa gcgccagcgg caggccccgg tggtcacagt
      841 gataaagatg tatttttcgc tcaggcaaat aaaccggctc tatctgtgcc gccagccgct
      901 ccaccgtcag cccgcactgc gcaagccact ggcggctttc actgctcacg cagtttgccc
      961 ggatttcccc ctccagcctg tccagcagcc ccaccagttc agccgggaac accacaccgt
     1021 gaccgtcttc gctcccttcc tgctgtaact tctccgccag gctgcggcgc tgcgctttct
     1081 cccgctcgcc gttctctgtc tcgacccgga tgagcggcgt gaagctcccc ggctggtata
     1141 ccgtctggat acgcgtggtg tcggtctgta ccgtcgtcag cctgtcgccg tcccagccgt
     1201 accatgtctc ctccggttta cgcgacagcg acatccaccc cgtcaggtca cgctcccgcc
     1261 gccatacccg tttcgccatt cgccgtccca gcgggtcgta gaggtagcgg ctctcgacca
     1321 gtggctcgcc atgctgtatc cgcgtgtgga acaccaggcg gtgctggctg tcgtagtggt
     1381 agtggtgggt ccgctcgtcg tccgtccgta tcacacccgt cgggatacgg tccgtcttct
     1441 ccgtcagtct gccgtattca tcatagtggt aaacatagtg cgcatcctct gcgatgcggt
     1501 tatccggcca cgccgtgagc gtgctgtccg ggtgtagctc cgggtccggc aggcggtttc
     1561 ccgccgggtc ggtggcatac gggatgcgga tgtccaggtc cggtgcgagg gtgcgcacgc
     1621 tctccagcct gcccgtggcg ctgtagccgt attcccgcgt ctgtcgcgga ccgctgatgc
     1681 gcaccaggtc gccgttgtca ttccacccgt agtcacggtc atataccagg ctgttcaggt
     1741 gctggctctg taactggcct gcgggggtgt atgtgctggt cagttcgtat gcggcattac
     1801 tgcctgccct gctgccgaag ctgcgcaccg tctcacggtg cagcctgtcc cgcgtatact
     1861 cgaccagcgg cgtcccgccc agcttcatgc ccgccaggta accgctgccg taggtcagcc
     1921 actccaccgg cggcaggctg tccggcgtga cgcggtttgc cagcccctgt tcgttgtagg
     1981 cgtgtttcgt ctcatgctgc cacagcagtt cccccgtctc cgggttctcc accgtctgcc
     2041 gttcgcccgt caggcggccc ttatcgtcgt agccgtagtg gaccgacacg cggtggcctt
     2101 cactggtatg gctgagggtg gttaaccacc cgtgctcatc atactgccac tgctctgccg
     2161 ggtcgccgtt caccgtccgg tgcgtgatgc ggtccgacgc atcgtagtgc cag
//
LOCUS       DAZFWP020000327         2207 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFWP020000327 DAZFWP020000000
VERSION     DAZFWP020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.329"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            49..858
                     /gene="yqeI"
                     /locus_tag="ACHK0Z_RS25730"
                     /old_locus_tag="pgaptmp_005146"
     CDS             49..858
                     /gene="yqeI"
                     /locus_tag="ACHK0Z_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4504983.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDTNIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            851..1333
                     /gene="yqeJ"
                     /locus_tag="ACHK0Z_RS25735"
                     /old_locus_tag="pgaptmp_005147"
     CDS             851..1333
                     /gene="yqeJ"
                     /locus_tag="ACHK0Z_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HGU4504984.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMFNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLVQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1366..1446)
                     /locus_tag="ACHK0Z_RS25740"
                     /old_locus_tag="pgaptmp_005148"
     CDS             complement(1366..1446)
                     /locus_tag="ACHK0Z_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504985.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1482..1906)
                     /gene="yqeK"
                     /locus_tag="ACHK0Z_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /pseudo
     CDS             complement(1482..1906)
                     /gene="yqeK"
                     /locus_tag="ACHK0Z_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            2100..>2207
                     /locus_tag="ACHK0Z_RS25750"
                     /old_locus_tag="pgaptmp_005150"
     CDS             2100..>2207
                     /locus_tag="ACHK0Z_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4504986.1"
                     /translation="MKPRNINNSLSLQPLVPDQENKNKKNEEKSVNPVKI"
BASE COUNT          773 a          393 c          374 g          667 t
ORIGIN      
        1 agcaaactat gtgatctcca tttcgattga tttagtgttt attgacgtat gtactggatt
       61 attaacgata atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc
      121 gatcttaacg tcgttctgac aacgccagcc agtcgatgtt tatcacttct acttgaagct
      181 tttcctgatg tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg
      241 cgtgtgccta ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct
      301 gttggtgata ctacccactc gctaattgca accgtgccga gaagaggatt caagatccat
      361 aatgacacta acatacaaaa tcatgtaata aactcgtcaa cagacgcaca tacacacaat
      421 gccccacctg ccataaaagt taatgcggga tacaaagaga gcattggtgg cgcaaagaat
      481 tttaataaca aaatcctcaa acatataaaa agtcatctaa ttatgttgag cgcatttgtc
      541 ataggcgcat attctgcata ttggctatgg aataataatc aaccgaagcc atttttcaag
      601 gattacaaga ctgttgcgga aattaatggc tgccatttta atgtaacaga agatacaatt
      661 gacgggttga aagagttcga taaatataaa acacggatac tggattcggg gattaactgt
      721 aaaaaacatc cgtggttata cttccctctt gcaaaatcct caccggggat gattgttatg
      781 gcatgcaata aaaattataa ccaacatgaa gtggcagact gtttaaccct gtcttaccgt
      841 gaggttaatc gtgattgatt ataaaaaaaa cttattattt attttagttt ttatcagtgg
      901 gtttattctc ttcactgtct attcttacac tgcagaaaaa atgatatata acgaaacatg
      961 cactgcaaat tgggtaattt ttaatgacca gggacgagca aatcttacca tcgactttat
     1021 gtttaacaaa aaaaataaaa caggaactgt tgcgttgagt ggaacctggc aacaaggtaa
     1081 cagagaaagt aaatcaatac ggcgaaatat tgaatacaca tggattgaaa actatgacac
     1141 agcccattta acatcaaaga aagttaataa gtttgagatt atggatcaag ttgatgatga
     1201 tagacttgta caacttattc ctgattttta tgtctttcca gaaaaaagtg taagctataa
     1261 tatactaaag caaggtaagc atgcttttat tttgagcatt ggtaacagag caataatgca
     1321 ttgtgcaagg taaactaaaa tagataacaa aaagatggaa cctcgttaat cataaaacaa
     1381 taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt gatcaacatc
     1441 tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca tctggtgcaa
     1501 atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg tacagtcgat
     1561 gtaaacaata cagaaagagg attattaata accagtttac tcagtgaatc aatagaggaa
     1621 aggattaacg tttctttaaa agaattaatt tcatcatctg ttaaactaaa ctcatcattg
     1681 acagatcgtg agatataact gtttttaact ttactcttta cgttgacttt attgacagaa
     1741 ttaacattca catatcttga atttaatgtc catgatgttg tttgcgtgag aaccttctca
     1801 gcattaatga attttttaac ggaatccttg gtttcttttt cgagtccgaa tttacaatat
     1861 catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta tacctcacat
     1921 ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact aaaattaata
     1981 cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata aggtgatatt
     2041 tgacattatc agaagctgcg aattcggatt ttgctctaat caaatgagag aaatgtaaaa
     2101 tgaagccacg aaatattaat aatagcctat cactgcaacc attagttcct gatcaggaga
     2161 acaaaaataa gaaaaatgaa gagaaatccg ttaatccagt taaaatc
//
LOCUS       DAZFWP020000328         2198 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFWP020000328 DAZFWP020000000
VERSION     DAZFWP020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.330"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            92..1414
                     /locus_tag="ACHK0Z_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             92..1414
                     /locus_tag="ACHK0Z_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU4504987.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1442..1753
                     /locus_tag="ACHK0Z_RS25760"
                     /old_locus_tag="pgaptmp_005152"
     CDS             1442..1753
                     /locus_tag="ACHK0Z_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGU4504988.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1809..>2198
                     /locus_tag="ACHK0Z_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             1809..>2198
                     /locus_tag="ACHK0Z_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU4504989.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESV"
BASE COUNT          622 a          448 c          468 g          660 t
ORIGIN      
        1 acctgaacta acttttcaca catcataaaa tggatatggc atacatgctt tattgggatg
       61 tataaatatc caaaacaaca ggtataaaaa aatgggatta atggcctcat tcgaacgtgg
      121 aatggaacgt tttcttgttc cagttgctat caagttaaac tcacaaaaac atgttgcagc
      181 ggtgagagat ggattcgttt ttacgtttcc aattatcatg gcaagttcat taattatatt
      241 aattaacttt gccatattat cgccagacgg ttttattgcc ggattactgc atctgaatag
      301 cattttcccc aaccttgaaa aagcacaagc tatttttact ccggtaatga atggttctgt
      361 aaatatcatg tcaattatga ttgctttcct ggtcgccagg aatatggcga ttagctatga
      421 gcaagatgat cttttatgcg gattaacggc aataggagca ttttttattg tttatacgcc
      481 atatcagatg atagatgggc aagcattcct gacgaccaaa tatctcggcg cgcaggggtt
      541 gtttgttgct gttatcgttg cattgatcac cagtgaaata ttttgtcgct tagctcgaaa
      601 cccaaaaatc accataacga tgccggcagc tgtaccgcct gcggtagcgc gttcatttaa
      661 agttttattg ccaatatttt ttgtcatggt gttcttttcc gcacttaatt attgcctgac
      721 actgatatcc ccggcaggac taaacgatct tatttacaca ttaatccaga cgccgctcaa
      781 acatatggga acgaatatct ttgcggtaat tatcctgggg gctgtgggta atttcctgtg
      841 ggtgctgggg atccacggac ctaataccac atcggcaatt cgagaaaccg ttttttctga
      901 ggctaatctg gagaatctct cctgggccgc tcaacatggc actacctggg gcgcgccata
      961 tccgattacc tggacttcta ttaatgatgc attcgccaac tgcggcggtt caggtatgac
     1021 gttggggtta ttgttggcta tttttatcgc ttctaagcgt gcggaatacc gtgatctggc
     1081 aaaaatgtca tttatccccg gtattttcaa tatcaatgaa ccgataatgt ttggccttcc
     1141 tattgtactt aaccccatca tgatggtgcc gtttattatg gttcccattg ttaactgtgc
     1201 cattggttac ttctttgttt cgatggaaat tattccaccg gttgcttatg ccgtgccttg
     1261 gactacgccc ggacctttaa ttgctttcct cggaaccggg gggaactggc tggctttact
     1321 ggttggtttt ttatgtttag gtgtggcgac aatgatctat ttaccttttg ttattgccgc
     1381 caacaaagtc aataacatga caactaacgg ataattccat ttacccagga aggagattgt
     1441 gatgtttgca gatgaagaac ttgttatgga gttactggtc aacgcggggc aggcacgttc
     1501 tgacgcgatg gaagccatac gttgtgccgg gcaaaaagac tggcagggcg caactcagct
     1561 aatggccagt tctgaatctg cctgtcttca ggcacataag attcagaccg cattaattag
     1621 tcaggatgaa ggctgtggca aaataaaagt taatttaatt ctgattcatg cacaggatca
     1681 tttgatgaat gcaatattat gccaggattt agccagggaa attatttcat taagaaaaga
     1741 actccatgcc tgattttttc ctccagtgcg atagctggag gaaattatta tactcttaag
     1801 gataagtcat gaatataaag acgcttaacg tcagcctttt gtctttttct attattacag
     1861 cattgtttcc attgaacgcg atggcaacaa aattaaccat agagcagcgc cttgaattgc
     1921 ttgaaaatga attgtcgcaa aataaacaag agctgaaagc aacacagaat gaactgggag
     1981 tatataaatc ccgactttcg accttacaaa aaagcatcac ggaaaacaaa tataaatcag
     2041 cctcgcttgc tgaaatatca gcgacatctc ccgtcgctga taacatcaaa aatgaaaacg
     2101 gtgaacagaa ctcgtttacc gcagcgcata ctataaatgg atcgcagcaa gttgccgtta
     2161 ttgaaagtaa aggcgacaaa accactatcg aaagcgtg
//
LOCUS       DAZFWP020000329         2197 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFWP020000329 DAZFWP020000000
VERSION     DAZFWP020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.331"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            353..646
                     /gene="bor"
                     /locus_tag="ACHK0Z_RS25770"
                     /old_locus_tag="pgaptmp_005154"
     CDS             353..646
                     /gene="bor"
                     /locus_tag="ACHK0Z_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU4504990.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(737..919)
                     /gene="rzoD"
                     /locus_tag="ACHK0Z_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             complement(737..919)
                     /gene="rzoD"
                     /locus_tag="ACHK0Z_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU4504991.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPRPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1136..1633)
                     /gene="rrrD"
                     /locus_tag="ACHK0Z_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             complement(1136..1633)
                     /gene="rrrD"
                     /locus_tag="ACHK0Z_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4504992.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(1633..1848)
                     /gene="essD"
                     /locus_tag="ACHK0Z_RS25785"
                     /old_locus_tag="pgaptmp_005157"
     CDS             complement(1633..1848)
                     /gene="essD"
                     /locus_tag="ACHK0Z_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4504993.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          609 a          525 c          461 g          602 t
ORIGIN      
        1 ttgataattc attactattt actgagagca ttcagaacac tacacaaatc tttccacgct
       61 aaatcataac gtccggtttc ttccgtgtca gcaccggggc gttggcataa tgcaatacgt
      121 gtacgcgcta aaccctgtgt gcatcgtttt taattattcc cggacactcc cgcagagaag
      181 ttccccgtca gggctgtgga catagttaat ccgggaatac aatgacgatt catcgcacct
      241 gacatacatt aataaatatt aacaatatga aatttcaact cattgtttag ggtttgttta
      301 attttctaca catacgattc tgcgaacttc aaaaagcatc gggaataaca ccatgaaaaa
      361 aatgctactc gctactgcgc tggccctgct tattacagga tgtgctcaac agacgtttac
      421 tgttcaaaac aaacagacag cagtagcacc aaaggaaacc atcacccatc atttcttcgt
      481 ttctggaatt gggcagaaga aaactgtcga tgcagccaaa atttgtggcg gcgcagaaaa
      541 tgttgttaaa acagaaaccc agcaaacatt cgtaaatgga ttgctcggtt ttattacttt
      601 aggcatttat actccgctgg aagcgcgtgt gtattgctca aaataattgc atgagttgcc
      661 catcgatatg gtcagctcta tctgcactgc tcattaatat acttctgggt tccttccagt
      721 tgtttttgca tagtgatcag cctctctctg agggtgaaat aatcccgttc agcggtgtct
      781 gccagtcggg gggaggctgc attatccacg ccggaggccg tggtggcttc acgcactgac
      841 tgacagactg ctttgatgtg caaccgacga cgaccagcgg caacatcatc acgcagagca
      901 tcattttcag ctttcgcatc agctaactcc ttcgtgtatt ttgcatcgag cgcagcaaca
      961 tcacgctgac gcatctgcat gtcagtaatt gccgcgttcg ctagcttcag ttctctggca
     1021 tttttgtcgc gctgggcttt gtaggcgatt gcgttatcac ggtaatgatt gaccgcccat
     1081 gacaggctga cgatgatgca gataatcaga gcggatataa tcgcggttac tctgctcact
     1141 gttgccccca caaacagact tcacgctcaa tctcacgacg agtcatcagg cctttccatt
     1201 gcttaccgcc agcgtatgtc cagcgacgca gctgatcaca tgcgcctttg atatcgccct
     1261 ggtttatttt gcgaagaagc gtcgatgttc taaaattgcc agcacccaca ttgtaaacga
     1321 atgagtaaag agcgccgcgc gttgtttccg gtatatcaac tttgatatac gggttaattt
     1381 gtctggcgac agtggcaagg tctttattca agagtgcttt gcattctgct ttggtatacg
     1441 ttttaccgag catgatgtct tttccggtgt gtccgtgaca tacagtccat acaccaacaa
     1501 tatctttgta tggtatgtag ctgacacctt ccagaccatc gttaccactt gggccagtga
     1561 ttaacactga tgctatagca attgctccgc caccaatagc agcagcaacg gcttttcgta
     1621 atgatggagg cattattcac ctctcgcagc cttgcgctta tcttctttaa tcttgaaata
     1681 aaggtttgtc aggtacgtca gcaggccaaa taccaggcta cccagcacac ctattgctgc
     1741 ccactgtgag ggagtgactt tatcgagcag ctgtaaaaac cagtaaccgg cactacctgc
     1801 tgaggtgcca taggcgactc cagttgttaa cttatccatg aatttcataa ccccacctcg
     1861 cagacaaagc gggtgtaaat tgagggaata caacgtatcg caaaaaagca gaaacgtaac
     1921 tgactccgag tcagtgaata actcaggtat tgagttatca gctaatatcg agactcaaaa
     1981 aatggaaaaa ccagctcgac ggcgggttta agctgtgtga cgaagtaacc actcttaaca
     2041 gcataaccaa ttttttacgt acgtaaacca ctgaatgata tttatgagaa tgctaccgag
     2101 tgttcaaaac atcaccacaa atacataaga aaacctcaac aaataaccaa taaataattt
     2161 ctagtgttat ttttagccgg tttaaattaa acagacg
//
LOCUS       DAZFWP020000330         2175 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFWP020000330 DAZFWP020000000
VERSION     DAZFWP020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.332"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            71..643
                     /locus_tag="ACHK0Z_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             71..643
                     /locus_tag="ACHK0Z_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4504994.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGRGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPENGVFIKNSGNSAVCTLFDSVSFSHLVREGGVHPL
                     TRETITPSMIVKPEDCIFDSSKGNFIIKDG"
     gene            861..1559
                     /gene="nleD"
                     /locus_tag="ACHK0Z_RS25795"
                     /old_locus_tag="pgaptmp_005159"
     CDS             861..1559
                     /gene="nleD"
                     /locus_tag="ACHK0Z_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HGU4504995.1"
                     /translation="MRPTSLNLALHQSSTSSSMSDADIESLVKTSSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVIAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRPESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSQGFLQVRLHPLL"
BASE COUNT          633 a          402 c          522 g          618 t
ORIGIN      
        1 ttgtcatgtc atggtgatgt ttgttaataa agacttgagg gaatatttat ccgtatgaag
       61 gaatatggta atgcctggat taatatcata tgtgtcatcg gcttcattcg tgaatgagat
      121 gatggagctg cgtcagcagg taatggaggg gcagattggt ggatttctcc tgggagggga
      181 gagggttaga gtttcttata tgccagatac aggccgtttt ttagcagaaa gtgaagggcg
      241 gggacgggtt tatgcagaat tattgaatat tgcttttaat gatggagtta atgtgctcag
      301 aaacaggata ttaagtgcgc tccctggaat gggagggcga aactctttgc aggaaaaaat
      361 atcggagtgt gcctttactg tcgatattga aaaacttcaa tgtcctggtg atgcgcttca
      421 atgtccaatt acactggagc agcctgaaaa tggtgttttc attaagaatt caggaaattc
      481 tgcggtttgc accctgtttg attcggtttc tttttctcat ttggtccgtg aaggtggggt
      541 tcacccactg acacgagaga caataactcc atcaatgatt gtaaagccag aagattgtat
      601 atttgacagt tcaaagggaa acttcattat aaaagatggt taaaatgttt tacatgaagc
      661 aatattgatt ttcggtagtt tatataaaac accacagaca ttcaaaatgt cagaggatgt
      721 cagtggtgga tgtacctgga gcggaagttg tggaagtaag ttatgttata tcgcaataaa
      781 ccgaatacat ctgtgatgtt acattggtct gagcttcagt gtgtggtgaa tgaaactcgt
      841 aacattttca gaggtccttg atgcgcccta cgtccctcaa cttggcatta catcagtcat
      901 caacgtcgag ttcaatgtca gatgcagata tcgagtctct tgtaaaaaca tcgagcgttc
      961 aatggataaa aaacaatccg caacttcgtt tccaggggac tgatcataat atatatcaac
     1021 agattgaagc agcgctcgat aagattggct ctacagagac agggcgtgta ctcctgaatg
     1081 ctattgaatc aatatcccga cttaaatcag aaacagtggt aatacacctc aattcttcca
     1141 gactaggagt tattgcacat agagatatag atgctgagaa ccatcgggga actggttccg
     1201 attttcactg taatctgaat gcagttgaat atccctgtgg ggaggggatt agcgtggtgg
     1261 actttcatgc gactattgtt tttcatgagc tgctccatgt gttccacaat ttaaatgggg
     1321 agcgcctgaa agttgagagt tcccgaccag aatcacaaaa atactctcca cttttactcg
     1381 aagaagccag aactgttggg ttgggggctt tttcagagga ggtgctttca gaaaataaat
     1441 tccgcgaaga gattgggatg ccccgtagaa catcctaccc tcacgactca gctctcattc
     1501 atgatgacaa tacagtgagt cagggattcc tacaggtaag actgcatcca ttgctttagg
     1561 tgtgtttctt gtggtgaata tatattggtg ggataaaact gagatacata aggtctcaca
     1621 atggcttgca aggctttgtg cttctctgta gaggcgacat gtttttatcc ggtgcgccgt
     1681 aaaaccccgt ccttcagggc gtggaggatg tcaaataata aggaatagtg gatatgtttg
     1741 cagcaaagcc cgagagcagt caaagaagta tttctggatt attcagtcct aggtcaaatg
     1801 gtgaaaacgc aagtaataaa ccgtcaagtg gtatggcgaa cagaatcaaa ccagttgtaa
     1861 gcgattcaca aatcggtaga tatacaactg acagtaatac tctgaaggat ggattgtgta
     1921 gccaagccca cataggaact caaccttatc ggccaggtgc tgactcgccg ctaactgtga
     1981 gatcattcct acaaattaca ctttccaaca ataattacaa atcatgagtc tgccctgtcc
     2041 cttctgccag gaagtgttac gcaacgatat ctcaccaatc tttcgcacca ctattaatca
     2101 atgagttaga ggtgattagt gacctgagac agagcattag cgcaaggtga ttttttgtcc
     2161 gtaagcgtaa accga
//
LOCUS       DAZFWP020000331         2121 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFWP020000331 DAZFWP020000000
VERSION     DAZFWP020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.333"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..803)
                     /locus_tag="ACHK0Z_RS25800"
                     /old_locus_tag="pgaptmp_005160"
     CDS             complement(<1..803)
                     /locus_tag="ACHK0Z_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4504996.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRHYTELQSEYKHLRRYFGVTAQVPYT"
     gene            complement(955..1152)
                     /locus_tag="ACHK0Z_RS25805"
                     /old_locus_tag="pgaptmp_005161"
     CDS             complement(955..1152)
                     /locus_tag="ACHK0Z_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4504997.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(1395..1925)
                     /locus_tag="ACHK0Z_RS25810"
                     /old_locus_tag="pgaptmp_005162"
     CDS             complement(1395..1925)
                     /locus_tag="ACHK0Z_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4504998.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRRMKKSGLDKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(1934..>2121)
                     /locus_tag="ACHK0Z_RS25815"
                     /old_locus_tag="pgaptmp_005163"
     CDS             complement(1934..>2121)
                     /locus_tag="ACHK0Z_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4504999.1"
                     /translation="IAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
BASE COUNT          501 a          601 c          450 g          569 t
ORIGIN      
        1 gtgtacggca cctgcgccgt cacaccgaaa taccgccgca gatgcttata ttcactctgc
       61 agttccgtat agtgtcggtt cagttcactg tatgtgctga ccagttggtg atgtggcttt
      121 tccagttctc cgcgctgatg cttctcttct gccacccggg caaacagcgc ctgtaatttc
      181 agataatcgc tttcgtccgg tagctgccac tgactggcac tgaaccagtg cgacaccatg
      241 tttttctttc ctgtggcatc tgcaatctgt tttgccgtta tccccagagc cgcacgtgca
      301 tcacgaaagt aagaaatcag cggggccatc acatgctgtt tcagtgccct gcccttcgcc
      361 tcatacccgg catctttcgg gcgatacggc ccctgataat gttccgcgaa cagaatgcgc
      421 tctgtggcgg ggaaatacgc ccgcaggctt tccttgttac acccgttcca gcgtccggac
      481 ggcttcgccc agataatatg gttcagcaca ctgaagcgtt cacgcatcat gatttcaatg
      541 tcagacgcca ggcgatggcc acagaacagg taaagacttc cggcaggttt cagcacccgc
      601 cagaactgcg ccagacactg gtccagccat ttcaggtaat cctcatcccc cgtccactgg
      661 ttatcccagc cctcaggctt cactttaaag tacggcgggt ccgtgactat caggtcaaca
      721 gaattttcgg gtaacgaccg gataaattcc aggcagtcgg cgttgattaa ttcacaactg
      781 gatattttta cagtgttaaa catggatcat taagcctgtc tctgataggc tcataccgct
      841 tttgcgcaaa gcagatgggc ctgaggtttg cttgtgatcc ggacgcatga gcagatggct
      901 ggtgagtgcc cctaacaccc accagccgcc catttaccac aaataaaaaa gccttcagga
      961 ctgaaggcgt ctgtaacaac cgaactgata gtctgccaga cccgccataa caagctgggt
     1021 cagtattagc tggcagcgtt cgcgtgaaag gtacgtattc tgtgcaatct ccccgactgt
     1081 cgccggtgcg gtggcactta attcattaaa caccactctg gcggtttctg tcatatcctg
     1141 ctgtttcagc atgtcttttt cccttttccg gttaacgtga cacaccaata actcttgtcg
     1201 aaaaagccag caagctgaaa gagcggtatt aataaccacc agcgaattta ttgcactact
     1261 gtatattgcg ggcacaaaaa aaccacccta cggtggcttc cttgtgcgaa aaaaacttgc
     1321 gtttcgcctc gcgatacagc tttgcgaagc ttacaggaat tcaagctgtt tctgcgtaaa
     1381 aaagcaagct ttttttatcg aaatgaatca tgcataggta cataaagcat gtgttcagcc
     1441 acggctaacc aacctgcaat acgtttctca catgtgctga agcaccattc cgggtgagta
     1501 cgatttaaac attctgccat ttttctctta ctcatacccc gtccttcgta cctttgccgg
     1561 agaatattga ttagcccggg atattcccca agcacctcac tgataacgcg atcaataatc
     1621 aaagcctctg tgtctgtaca atgtgacaac cagctcttct gctttcctct tgtcatatcc
     1681 cgaaaaaatg cctcaagttc cggtttatcc agcccggatt tcttcattct gcgtaaaacc
     1741 tcattaactg cggttttcgt cagttttttc gaaaccagta accggttaaa catatttccg
     1801 gatttacccc cacctatata cgaccaccgc ccccacatcc gtaatttccc ctggatccag
     1861 actgcttcca gcgtgttcag gcgtaaatgt tcgccgcttt tgcctgtaat ttccggatat
     1921 atcatattta cgctcactca ctctcaattt tgtaaatctt cacacccagc cgtccaccag
     1981 atactggctg accgcgcaca atattgattt catcaaactg ctcgtcatca atgagcactt
     2041 ccgcatgcgt cagcgcatcc agcggtgctt tcagaatgtt gtccaggtca cggcggcgct
     2101 tatccggtgg ctctgcaatc a
//
LOCUS       DAZFWP020000332         2114 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFWP020000332 DAZFWP020000000
VERSION     DAZFWP020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.334"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1211
                     /locus_tag="ACHK0Z_RS25820"
                     /old_locus_tag="pgaptmp_005164"
     CDS             <1..1211
                     /locus_tag="ACHK0Z_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4505000.1"
                     /translation="LPRGCLDSVLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQ
                     EEAVSALLRYDTGVLCAPTAFGKTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVF
                     LQAGDSIGIIGGGKHKPCGNIDIAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSF
                     SAILKETNARYLLGLTATPIRRDGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRF
                     TSGHLPSDARIQDIFREIALDHDRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASV
                     MNSLKLSPFILHGRLSKKKRAMLISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLI
                     LAMPVSWKGTLQQYAGRLHREHTGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYR
                     IIADGDESVI"
     gene            complement(1373..1549)
                     /locus_tag="ACHK0Z_RS25825"
                     /old_locus_tag="pgaptmp_005165"
     CDS             complement(1373..1549)
                     /locus_tag="ACHK0Z_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505001.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(1687..1929)
                     /locus_tag="ACHK0Z_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /pseudo
     CDS             complement(1687..1929)
                     /locus_tag="ACHK0Z_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1968..>2114)
                     /locus_tag="ACHK0Z_RS25835"
                     /old_locus_tag="pgaptmp_005167"
     CDS             complement(1968..>2114)
                     /locus_tag="ACHK0Z_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGU4505002.1"
                     /translation="LLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          521 a          453 c          548 g          592 t
ORIGIN      
        1 cgttgccccg gggatgtctg gacagcgtat tatctttcct tagggacaac aatattgctg
       61 cagaattaat cgataaacga tttgccggga cggaatgtaa tgccgttttt atgggaaacc
      121 tcagagcgga gcaggaagag gccgtttcgg cattactccg ttatgacact ggtgtgcttt
      181 gtgcgccaac ggcttttggt aagacagtta ccgcagcggc agtgattgcc aagcggaaag
      241 tgaatacact gatactggta caccggactg aattgctgaa gcagtggcag gagcgtctcg
      301 cggtgtttct gcaggccggt gacagtattg gtattatcgg gggaggtaaa cataaaccct
      361 gtggcaatat tgatattgcg gtggtgcagt ccatatccag acaaggagaa gttgaacctc
      421 tggtcaggaa ttatgggcaa atcattgtgg atgagtgcca tcatattggc gcggtttcat
      481 tttctgcgat tctgaaggaa acgaatgcca gatatctgct tggcctgacg gcaacaccaa
      541 tccgacggga tggtctgcat cccattattt ttatgtactg tggtgccatt cgccatacag
      601 cggtccgccc gaaggaaagc ccacataatc tggaggtact gatccgttcc cgttttacat
      661 ctggtcattt accatcggat gcgagaatcc aggatatttt cagagaaatt gctctggatc
      721 atgacagaac ggtggcgata gctgaagaag ccatgaaagc tttcgggcag gggcgaaaag
      781 ttctggtact gactgaacgt acagatcatc tggatgagat agcatcagtg atgaattcac
      841 tgaaattgtc tccctttatt ctccatggtc gactatcgaa aaaaaagcgt gcgatgctga
      901 tatccgggct gaatgctctt cctcccgatt ctcctcgaat tttgttgtca acaggcagac
      961 ttattggtga gggatttgac caccctccgc tggatacgct gattcttgcc atgcctgtgt
     1021 catggaaagg gacattacag cagtatgcag ggcgtcttca cagagagcat accggtaaaa
     1081 gcgatgtcag gatcattgat tttgtggata ccgcgtatcc tgtgctgctc agaatgtggg
     1141 ataaacgtca gcggggttat aaagcgatgg ggtacaggat tatagctgac ggcgatgaat
     1201 cagtgattta attttaagga gtactttacc ctttttaaat aatttgagct ccccgggttg
     1261 ttcgggcaac aaagagtccc agtatacagg cttcaggaat atgttgtagt atcaggcttt
     1321 tcatcgcttt aaaatgacta ccggcagtga gaacgtcatc aaaaataaca atctagggcc
     1381 tgcacccttc cagtaattgt tcatccagtt ggtagtttgc cataatcgct tctggtaagg
     1441 gccggtttgt tgcggtatga gaggctattg ttgatacgtt ttgcacaacc agttcccaga
     1501 aatccagcca tggttcgtag gtttgcgcaa ttcgcagaat ttctgtcata cggtcatcgt
     1561 aagcggcatc tgtacggcat ttggatggtg gtacgggaac aaatgtgtat tcctcaagat
     1621 taccgatatt tgatatgagc atctgagcaa catactcaat agcagccttt ttatagtggt
     1681 aatcaggcta cgaaagtaca gcgttttata aattgtctca ggtgatattc gcagcgtttt
     1741 ttgacgtggt tttgttcgcc ttaaccatcc tgatatttgc tctggagacc atttcatctc
     1801 cagcttttcc agaacaagct ttcgcaatgg taaattttga tccagtaagc acggttttgg
     1861 ccttttcgcc attctgttgg ctcggttatt agcatcaaca gctttgtaat agcgtctgcc
     1921 ccgattacgc gtaagcgtac agcgagggcc gtattgacgg ggatgtgtta ttcagctggc
     1981 agtgctatgc gccacggaag cagttcgctg acccggttga ccggccagtc tgctatgacg
     2041 ccaagcacat ggcgaaggta gctttctgga tccacgtcat tcagtttgca cgtcccgatc
     2101 aggctgtaca gtag
//
LOCUS       DAZFWP020000333         2100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFWP020000333 DAZFWP020000000
VERSION     DAZFWP020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.335"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..570)
                     /locus_tag="ACHK0Z_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /pseudo
     CDS             complement(1..570)
                     /locus_tag="ACHK0Z_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(530..649)
                     /locus_tag="ACHK0Z_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /pseudo
     CDS             complement(530..649)
                     /locus_tag="ACHK0Z_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(662..>2100)
                     /locus_tag="ACHK0Z_RS25850"
                     /old_locus_tag="pgaptmp_005170"
     CDS             complement(662..>2100)
                     /locus_tag="ACHK0Z_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505003.1"
                     /translation="RLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT          549 a          464 c          373 g          714 t
ORIGIN      
        1 aataaaacat caccaacttc aatatcaaca agacttctgt aacatgtttt actaaatcca
       61 attacaaaat gaatggacca atataatgat tttttgtcta acactggagc gtttccgtat
      121 tggcagactg tgactttatc taaccaaaga ttcaattccc ctgttcatat tcttattagt
      181 ggttgagcag ggttcaatac tccatcaaat actttaccta cgtgcaaacg attatatttt
      241 agttcaggat gctttagatt taacggggaa ctggataaac tgagccattc ccctattaac
      301 ctttccgttt ctggcattaa agccatccca gcgagctcag atgaaattaa gggaaatatt
      361 tctccaagca atacccatcc agaccagctg ccactctctt ctgctatcgg taaccattta
      421 gcactaaatt cgggcattaa taatgcaaca tcttcgccat tttctttcca attttgaaaa
      481 gtggtttcaa acgtatgagt gtttcgattt actttcctca gaccgaacat taactttcat
      541 ccttctgatc gttttcgaat ttgctttcat tttcttgctc ctcataaggc tgatgtcgtt
      601 gtccttgtcg ttcatcttgc tcagtcaata cccaacgttg tgctgtaact cattatgaaa
      661 actatttaat tccctcatca ttgataaggt tggacatctc tttcaacatg agagtcattg
      721 aagctttcga ccaatttagc ggaatgatag ggcttggcgc ttcatgcaaa gttcccgact
      781 tgtggatata gttatagctt tcgggaagtg ccatttcagg tacactcctg ccatttgcac
      841 caatcatatt ctcgcctgtt atttgggcca atgatctatt cataaattgc actgagtcat
      901 tcaaatactc ttcctttctt gattctttat aaacaattgc tgctgatttt gcataccatg
      961 agtcaaaaaa ccattctgct tctgttgatg gaatgaagct cttttctctt ttcgcatgtg
     1021 aattttgatc ggtatcggtt ttaatatcat tgaaccaaaa atttgccgac tgataattat
     1081 ccttctcgta tctttttata ccatacggac ctgctagttt tttaactatt tttagaacct
     1141 gctcttttct tctggtgttt atctttgcaa gatttgcagg ataaataaca ttaagtaaag
     1201 ccgcatcggc ttctctgtaa tgtttatctt tttccagata acctggggat tctcccccta
     1261 aatcaagctg taacgttatt ctttcataac ctttatctat gaggtggttc aacctcgttg
     1321 ttgaaagagg ttcatccagt tcattggctt tagcttcgcg tagtaaatca gagacaaaaa
     1381 cagagtcttt cttcgagagt aagttcgaca aacgttccag tccagaggtc accagtgcaa
     1441 cggaagatgt gtttaatcgg gcatcttctt cccatgctcc ggaatcttcc ataacataga
     1501 aattggcttt atccagataa gcaatcagca gcgcaaccga ttttaatcgg tcaccttttt
     1561 gccaatcttc agcgttaatt gtatcagtgt cgattgcttg tataaggaga tcaagataaa
     1621 gacctaacgc atcattttgt ttatggttcc acagctgcgg cttgccttct tcctgcacat
     1681 ctgccatcac gggggagttg ctgtcaaatc ggatatgcac aacattcatt tgcccgggaa
     1741 ctccatccag tcgtttgggg ttgctaatga cgtcctgcat ccgcttaatc tggtcgggcg
     1801 tagacatata atcccacagg gtgagcagaa ccttttttgc ggctacacta tttccctgat
     1861 ctgatactaa tgccatatag ccccataaac tatccctcag ccagacagca tcatagtggg
     1921 tttctgccgc tgttttgtca tcttcgaaag tagaagcaag aataaacgtt ccccagggtt
     1981 tctccgtcaa cgttaacttt cgtgtgtttt ctgttaatgt tagcaatcct tgaatttcgt
     2041 ctttggtata gtggctcttt atttcatttt gtaatttggc tgagtgaatc aaaagacgat
//
LOCUS       DAZFWP020000334         2098 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFWP020000334 DAZFWP020000000
VERSION     DAZFWP020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.336"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..446
                     /locus_tag="ACHK0Z_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /pseudo
     CDS             3..446
                     /locus_tag="ACHK0Z_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            538..1766
                     /locus_tag="ACHK0Z_RS25860"
                     /old_locus_tag="pgaptmp_005172"
     CDS             join(538..853,853..1766)
                     /locus_tag="ACHK0Z_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HGU4505004.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLYVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPTVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQWACNGLSDNRCLEI"
     gene            1779..2098
                     /locus_tag="ACHK0Z_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /pseudo
     CDS             1779..2098
                     /locus_tag="ACHK0Z_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          581 a          479 c          548 g          490 t
ORIGIN      
        1 atatcctttc cactctgtca ttatgcattt ctgggatagt ttctactgca accgcaactt
       61 cttcagaaac aaaaatcagc aacgaagaga cgctcgtcgt gaccacgaat cgttcggcaa
      121 gcaacctttg ggaaagcccg gcgactatac aggttattga ccaacaaaca ttgcagaact
      181 ccaccaatgc ctccatagcc gataatttgc aggacatccc cggagtagag ataacagaca
      241 actccttggc aggccgtaaa caaatccgca ttcgtggcga agcatcctcc cgtgttttaa
      301 ttctcattga tggtcaggag gtaacttatc agcgcgccgg agataattat ggtgtgggac
      361 tgttgataga tgagtctgcg ctggagcgtg ttgaggtagt gaaaggtcca tattccgtac
      421 tgtacggttc acaggcaatt ggcggttaga ctggccccct gaatctccag acaaccagta
      481 tcacttaaat aagtgatagt cttaatacta gtttttagac tagtcattgg agaacagatg
      541 attgatgtct tagggccgga gaaacgcaga cggcgtacca cacaggaaaa gatcgcaatt
      601 gttcagcaga gctttgaacc ggggatgacg gtctccctcg ttgcccggca acatggtgta
      661 gcagccagcc agttatttct ctggcgtaag caataccagg aaggaagtct tactgctgtc
      721 gccgccggag aacaggttgt tcctgcctct gaacttgctg ccgccatgaa gcagattaaa
      781 gaactccagc gcctgctcgg caagaaaacg atggaaaatg aactcctcaa agaagccgtt
      841 gaatatggac gggcaaaaaa gtggatagcg cacgcgccct tattgcccgg ggatggggag
      901 taagcttagt cagccgttgt ctccgggtgt cgcgtgcgca gttgtacgtc attctcagac
      961 gaaccgatga ctggatggat ggccgccgca gtcgtcacac tgatgatacg gatgtgcttc
     1021 tccgtataca ccatgttatc ggagagctgc ccacgtatgg ttatcgtcgg gtatgggcgc
     1081 tgcttcgcag acaggcagaa cttgatggta tgcctgcgat caatgccaaa cgtgtttacc
     1141 ggatcatgcg ccagaatgcg ctgttgcttg agcgaaaacc tactgtaccg ccatcgaaac
     1201 gggcacatac aggcagagtg gccgtgaaag aaagcaatca gcgatggtgc tctgacgggt
     1261 tcgagttctg ctgtgataac ggagagagac tgcgtgtcac gttcgcgctg gactgctgtg
     1321 atcgtgaggc actgcactgg gcggtgacta ccggcggctt caacagtgaa acagtacagg
     1381 acgtcatgct gggagcggtg gaacgccgct tcggcaacga tcttccgtcg tctccagtgg
     1441 agtggctgac ggataatggt tcatgctacc gggctaatga aacacgccag ttcgcccgga
     1501 tgttgggact tgaaccgaag aacacggcgg tgcggagtcc ggagagtaac ggaatagcag
     1561 agagcttcgt gaaaacgata aagcgtgact acatcagtat catgcccaaa ccagacgggt
     1621 taacggcagc aaagaacctt gcagaggcgt tcgagcatta taacgaatgg catccgcata
     1681 gtgcgctggg ttatcgctcg ccacgggaat atctgcggca gtgggcttgt aatgggttaa
     1741 gtgataacag atgtctggaa atataggggc aaatccactt ttcaaatgga gaaattaata
     1801 atctcttcat taaaaaaagc aacatatttg gagcatcatt cactaatacc agaataaaaa
     1861 atttgctctg tgtagatatt atgcctggtc gatggaccac tcaacttgtc aataagcact
     1921 taggttacag atacacagga gtattcaaaa ccctggcaag tattgatgac aaaccgtccc
     1981 gtttcgaaat tttgatacca ttggtacaaa ctttagttcg cgacaatgta aagctaaata
     2041 atgatgtgta taaagaactt aataagttta tgcacgatta cgacaaaaca tcatcaga
//
LOCUS       DAZFWP020000335         2091 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFWP020000335 DAZFWP020000000
VERSION     DAZFWP020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.337"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1154
                     /locus_tag="ACHK0Z_RS25870"
                     /old_locus_tag="pgaptmp_005174"
     CDS             join(<1..235,235..1154)
                     /locus_tag="ACHK0Z_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4505005.1"
                     /translation="FTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRE
                     RQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERFTIAARLSD
                     SHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEM
                     LTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQFAVPEPDRV
                     WCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAYETRGQPRD
                     VVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKTEWVPTNGY
                     AGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT"
     gene            1164..1715
                     /locus_tag="ACHK0Z_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /pseudo
     CDS             1164..1715
                     /locus_tag="ACHK0Z_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            1715..1960
                     /locus_tag="ACHK0Z_RS25880"
                     /old_locus_tag="pgaptmp_005176"
     CDS             1715..1960
                     /locus_tag="ACHK0Z_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505006.1"
                     /translation="MQHVSEGVIQVPELPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQQNIIHP"
BASE COUNT          511 a          551 c          576 g          453 t
ORIGIN      
        1 ttcacccctg agttcaggct ggaatgtgca cagctgattg ttgataaggg ctactcatat
       61 cgacaggcca gtgaagcgat gaatgtcggt tcaaccacgc ttgagagctg ggtacgccag
      121 ctcaggcgag agcgccaggg tattacgccc tctgccacac ccattactcc agaccagcaa
      181 cgtatccgcg agctggaaaa gcaagttcgc cgtctggagg aacaaaatac gatattaaaa
      241 aaggctaccg cgctcttaat gtccgactcg ctgaacggtt cacgatagcc gccagactaa
      301 gtgacagcca cacggttgtc agcctgtgtt ctgctctgga aatacaccgc agcagttacc
      361 ggtactggcg aaaacgacgc gatacggtta atccggcacg agtcaggttg tgcagcgaaa
      421 tacgccgggc gtggaaccaa agtcggggct ctgcgggcgc gcgcactctg gctgaaatgc
      481 tgactcaaaa cggcgtcccg atgagccgtt accgtgccgg acgtctgatg aaatatctga
      541 acctgagcag ttgtcagccc ggaaaacatc agtataaaaa tgcccgtcag gagcatacca
      601 gcctgccgaa tctgcttgag cgtcagttcg cagtaccgga gccagaccgg gtatggtgtg
      661 gagatattac gtatctctgg gcaggaaatc gctggtgcta tctggcggtt gttatggatc
      721 tttttgcccg cagggttatc ggctggagtc tgtcagcgca tgccgatacc gcactgataa
      781 gcagtgccct gcggatggcc tatgagacgc gtggccaacc ccgggatgtc gtgttccata
      841 gcgaccaggg aagccagtat acaggcctta aatatcaaca acttctctgg cgttgcagaa
      901 taaatcaaag cgtcagtcgg cggggaaact gctgggataa cagccccatg gaacgcttct
      961 tccgcagtct gaaaacagaa tgggtgccaa cgaatggtta cgcaggcaag gacgaggccc
     1021 ggcagcaaat taatgattac atattgaact actacaacag cgtcagacct caccattata
     1081 acggtgggct gacgccggaa gagtcagaga acagatacca tttttactgt aaaaccgtgg
     1141 ccaatattac ttgaccacta cacggagaga taaacggaga acatgtccct gaaattattc
     1201 tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac ttcaggttcg
     1261 tctgccagaa cggatgcgtc tgtggtcagt ctctggggga agtgcgtgtt ccgcaccggg
     1321 gaaatgtggt ggacagagtc attgaagggg cttacgaggt ggtgggcgtg tttgaccgga
     1381 tagaggagaa gcgtgatgcc atgcagtcgc tgctcttacc gccaccggca cgccaggcgc
     1441 tggcacaggc ggcactgact taccgttatg gtgatgaaca tcagcccgtc accactgccg
     1501 acattctgac accacgacgc cgggaggatt acggtcagga cctgtggagt gcttatcaga
     1561 ccattcagga gaatatgctg aaaggcggaa tttccggtcg cagtgccaga ggaaaacgta
     1621 tccacacccg tgccattcac agcatcgaca ccgatattaa gctcaaccgc gcattgtggg
     1681 taatggcaga aacgctgctg gagagcctgc gctgatgcag catgtctctg aaggcgtgat
     1741 tcaggtacca gaattacccg acgccccgga ggcgctgccg gggtgtcgtt ccatttctga
     1801 tgatgacctg tgcgcctggt gtacccgtct gctttaccgt cccggagaac tcagtctgtg
     1861 cagactgagt acaggcaatg gcatctggcc atccgtctgt gaccggaacg gttacgcgta
     1921 cggctgtccg gaatttcagc agaatatcat tcatccctga tattaccggg tctcacggtc
     1981 agtgatgaac gtgatgttgc cagctcccga cctttactcc ctctcattta ttcacattac
     2041 aaggatttca tacatgaaaa cattgtctca gaacaccacc tcttcggcct g
//
LOCUS       DAZFWP020000336         2054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFWP020000336 DAZFWP020000000
VERSION     DAZFWP020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.339"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(190..743)
                     /locus_tag="ACHK0Z_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /pseudo
     CDS             complement(190..743)
                     /locus_tag="ACHK0Z_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            771..1319
                     /locus_tag="ACHK0Z_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             771..1319
                     /locus_tag="ACHK0Z_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505007.1"
                     /translation="MMPVARYLCIFINVGLGEGSALPVGVPVPWPSVTPPTGWLKCNG
                     AAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDDGRGIDTGRALLNWQPHTILDHAHYM
                     ELWTGDGLAAGSAREGVNPGILATYGDGGIVKTDEPGLNVPSSLRAISSRSVKRYGEI
                     SENVGTETRPRNIAFNYIVRAA"
     gene            1319..1921
                     /gene="tfaE"
                     /locus_tag="ACHK0Z_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             1319..1921
                     /gene="tfaE"
                     /locus_tag="ACHK0Z_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4505008.1"
                     /translation="MNKAVLNSELITTKAGDITVYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGSYKAGYAICRSADFNSWEYVPDHRGETVYSTETGESKEITAPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1893..>2054)
                     /locus_tag="ACHK0Z_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             complement(1893..>2054)
                     /locus_tag="ACHK0Z_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4505009.1"
                     /translation="SRQWPGKAAIGRLKGDELEQYNLWLDYLDALELVDISSAPDIEW
                     PTPPAVQAR"
BASE COUNT          555 a          499 c          487 g          513 t
ORIGIN      
        1 gataaaaaat attgatgcag agctaagaga tgctgtcaat aagggattcg acattatcga
       61 ccaagaagta aagtcactac aaatgaaatt aaacaaatta gcttcataga atgctccact
      121 ccctgttcaa tatttttaca agcttatcag ccagccgtgg cacgttcttg catacgacgt
      181 gctacggttt catttatctc cgaccggaaa cttcttatac aaagtcgata cgccaacatc
      241 atagatgatc tccacattct ggcgaggaac gcctgatgca attagtcgcc cggcctgcgc
      301 ccattgttct gatgaaagtt tgggacggcg accgccaatt cgtccctgtg cgcgagcagc
      361 ttccagtcca gcttttgttc gttcaacaat cagttcacgc tccatttcag ccagggcacc
      421 catgacatga aaaaagaaac ggccattggg gtgctggtat caatactgtc agtcaggctt
      481 cggaaattca cgccacgctg acgcaactct tctatcagcg taacaagatg ccgcatactg
      541 cgccccaacc tgtccagctt ccagacaacc agagtgtctc ctgccgatag tgtcctgagc
      601 agttttttaa gccccggtct gtcggactta gtgccactga ttttatcctc aaaaaaccgc
      661 tcacatcccg cgcagttcag tgcattacgt tgcagatcgg tgttctggtc atttgttgat
      721 acgcgtacat agccaataag catgatggat cccctgaata aaaaccggag atgatgccag
      781 ttgcccgtta tctctgcatt ttcataaacg ttggtttggg agaaggttct gcattacctg
      841 ttggtgtgcc tgttccgtgg ccttcagtca caccgccaac aggctggctg aaatgcaacg
      901 gtgcggcttt ttctgctgaa gaatacccgg aactggcaaa ggcttacccg accaataaat
      961 tgcctgattt acgcggtgaa tttattcgtg gctgggatga cggtcgcggg attgatactg
     1021 gtcgcgcttt gcttaattgg cagccacaca caattttgga ccatgcacac tatatggaat
     1081 tatggacagg ggacggactc gccgcaggaa gtgcacggga aggagtaaac ccaggaatac
     1141 tggctacata cggtgacggg ggaatagtta aaacggacga acccggtctt aatgtgcctt
     1201 cctcactacg agctattagc tctcgtagtg ttaaacgtta tggtgaaatt agtgaaaatg
     1261 taggtacaga aactcgtcct cgtaacatcg cttttaatta tatcgtaagg gccgcatgat
     1321 gaataaagct gtattaaata gcgaactcat caccacaaag gcgggagaca ttaccgttta
     1381 caattatgat ggtgagacac gggaatatat ttccacatca actgaatatc ttgctgtggg
     1441 tgtcggtatc ccggcatgtt cctgtttaga tgctcctggc tcatataaag ctggttatgc
     1501 aatttgccgt tctgcagatt ttaactcatg ggaatatgtg ccagaccatc gcggtgaaac
     1561 ggtctatagc actgaaacag gagaatcaaa agaaatcaca gctccgggtg attaccctga
     1621 aaatacaacc actatcgctc cattaacgcc atacgataaa tgggatggtg agaaatgggt
     1681 gaccgatact gaggcacagc atagcgcagc agtcgaagcg gcagaagcac agcgtcagtc
     1741 actgattgat gctgcaatgg cttccattag tctgattcaa ctgaaattac aggccgggcg
     1801 gaagctgacg caggcagaaa caacccggct taacgctgtg ctggattaca ttgacgcggt
     1861 gactgcaaca gataccagca ccgcgccgga tgtcatctgg cctgaactgc cggaggcgta
     1921 ggccattcaa tatctggagc actggagata tcaaccagtt ccagtgcgtc cagataatcc
     1981 agccataaat tatattgttc cagctcgtca cctttcagac gaccaatagc ggctttacca
     2041 ggccattgcc tgct
//
LOCUS       DAZFWP020000337         2010 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFWP020000337 DAZFWP020000000
VERSION     DAZFWP020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.340"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..731)
                     /locus_tag="ACHK0Z_RS25905"
                     /old_locus_tag="pgaptmp_005181"
     CDS             complement(<1..731)
                     /locus_tag="ACHK0Z_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4505010.1"
                     /translation="MWNLLRRTRKNQKSGRDVREVGWRSLFQAVAEPFAGAWQQGVKA
                     DPETVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWNRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNG
                     GRPSGVIEVPGSITEENAKKLKGNW"
     gene            complement(677..898)
                     /locus_tag="ACHK0Z_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             complement(677..898)
                     /locus_tag="ACHK0Z_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505011.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(943..>2010)
                     /locus_tag="ACHK0Z_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             complement(943..>2010)
                     /locus_tag="ACHK0Z_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4505012.1"
                     /translation="KGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVTANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
BASE COUNT          415 a          665 c          518 g          412 t
ORIGIN      
        1 tcccagttcc ccttcagttt tttcgcgttt tcttccgtaa tactgccggg gacctcaatc
       61 acgcctgatg gtcgcccgcc atttctgaaa aaatacgtcg aatttgcctg aatatgatgc
      121 ccctgcatgg cggccagtcc ggcggcatac accggcggca gccccacaag cggatgaaaa
      181 aaacagttaa accggtcgtg gatcacttcc cgggcaggca ccgtcaccgc ctccgtgatc
      241 ccgcagttcc ggtccggcgt gatgcgatag aacacctcgc cgtcatccgc caccagaggt
      301 tcaacccggt tccagtccag aatacgcagt tctttgatct gcccccggga gttacggatt
      361 ttcagcacca ccgtattgcc gtgacgcagt ttggcgttca gccacagttc aaaaaactgg
      421 atacgattct gctgtgcatt gggacgacga cagagacggg caatatcccc ctgccgtttt
      481 tcacggcgga tcccctgtgt atcggtctgc atcaggcgca gtcgcatttt ggcgatatcc
      541 tgggatatca gcgaaatgca agaaaacacc gcgtgaaagg acaaaacggt ttccggatcg
      601 gctttcacac cctgctgcca ggcaccggca aaaggctcag ccaccgcctg aaacagggac
      661 cgccagccca cttctcttac gtcacgtcct gatttctggt tttttcgggt tcgccgcaaa
      721 aggttccaca ttcgccatgc tccgcatcac gtttcttttt ctgacctgcc ggacgtcgcg
      781 ctgtgatgta ctccgccttc cccaggcgaa ccagcacctc cgcacacggc tgtgccacat
      841 cacggatatc cccggcccgg gcatcatgcg tgccctgcag atactggatt tttgccatct
      901 gttactgcgg gaagctcgcg cctcccgccc tcctcatcag actcagccac cggacgcagt
      961 tccgtagttc acaccggtga tcaccgccac tgccgcggta cggcgacgac gccagttgat
     1021 ccagcgctcc gcacggatgg ccacgctgcc ggtctgaaac atggagacca gttccaccgg
     1081 tgacggtgtg ctgctgtcgc tggtcggttc agactgcatc tccagtgacg cttcacggga
     1141 catatccacc gccacaccgc cgtcatcagc cagataaata tccggtgcat tcaccagtac
     1201 cagctggtca cccacatact gggagacaat caccggaagc ccctggaagg tcccgcccag
     1261 caaggtcatg tccggatatt ccttctgccc cagcgcattt ttacgcatgg acagcgccag
     1321 ggcattcgtg ctggacatca gccagaccgc accggtgggc tgcagatttg ccgtcacaaa
     1381 ctggccaaac gcggcctcgg catccgcatc cgggttaccg gttgatgccg tgcccttcac
     1441 atcatgggtg atggacgccg gggagacatc cgccaccgcc gcttttttcg ggtccacaaa
     1501 gtctgtgtcc agacgcgcca ccaccgcttc tgccagcgca ttacggacca gcgcatcagc
     1561 tgccggactg gaaaaacgga tcagctcttc cgtcagtacc gcaatggccg acaccttcgc
     1621 atgactgaag gtgatggatt caaaatcaaa cttcgtcagg ggtctggcct taccctcccc
     1681 cacccagccg gcagcaccac cggacacctg ggcatgcacg cggatattga acggcacctg
     1741 acgaagtgca gggatcccgc cctgaccaaa tcgcccgata atggtctgcg gacgcaggta
     1801 atcaataaag tcctgcgcat attcctggta ttcagacagg ctgcctgccc actgcgggtc
     1861 cgtggtggtc cctgccccca ccgccgattt caggacatga tgcagacggc tgtcatccgg
     1921 atactgacga cgggccactt ccagggcttc agagcggaca cctttagccg cggccagtga
     1981 tttggcaaag cgggcgaaac caatcccctt
//
LOCUS       DAZFWP020000338         2001 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFWP020000338 DAZFWP020000000
VERSION     DAZFWP020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.341"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1907)
                     /gene="rhsD"
                     /locus_tag="ACHK0Z_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             complement(<1..1907)
                     /gene="rhsD"
                     /locus_tag="ACHK0Z_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HGU4505013.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLT"
BASE COUNT          326 a          691 c          581 g          403 t
ORIGIN      
        1 gtcagcccgc cctgcgtggt gctgaccgcc tttccccacg catcgtactg tgtctcgctc
       61 cggttgccgt ccggggtgat aacggcagtc aggtcgcctg cggcgttgta ctcatactgc
      121 gtttcacggc cctgtgcgtc tttcaccgag gttaaccggc cacggttgtc atagtggcgg
      181 taaaggctga taccttcctc gcggtgaacc gccgtcatct ggccgaagcg gtcgtattca
      241 tagcgggtct ggtagcccga gcagtcagtg aacgccagca actgcccgta gcggctccac
      301 gtcatctgcc gggtgctgcc cgtcgcatcc gttgtcgtcg ccggtaactc actgtgcgcg
      361 tcatcgtagc ggtagcgtac tgtctccccg ctgcgcgatg tctccgatac cagcctgccc
      421 ggttcatcat attcccggcg gctctccagc ccgtccgggg acaccaccgc cgtcagctgg
      481 ttcccgtcgt tatagtaaaa tttcgtctcc cgcccgtccg gtgtggtgat gtccgtgata
      541 tcgccggaca ccacattcag accgtactct gtccgccgtc ccgccgcgtc cgtctgcgcc
      601 gtgagccttc ctgccgcgtc atacccgctg tgcgtgacgc tgccgtccgc cagttctttt
      661 ttcaccaccc gcttcagccc ggccccgcct tctgtatgca gcacctcacg ccggttcagg
      721 ctgtccgtga cggtgatgcg gtcctgctca tactggtagc ggtaactcag gcctgccggg
      781 ttcagctgct ccaccacccg cccggtatcg tcgtagcggt agcgcatctc cggccttccc
      841 gcgtaacggt gcgccaccat ccggcccggg tgctgcgcgt catacgtgaa agcgcgcacc
      901 tgcgtattgc tgcggtcata taccgccagc agttcaccgg cttccgtata cgtgtaccgc
      961 gccagtggcg caccgggcag gctttccggg tatgccgggt cgtgcatcag ccacaccgcc
     1021 gaaagacgga tgcccctgtc ggggccgtat tcggtaccgg gcagtgtgtc ggggaacggt
     1081 gaggctgaga gagggcggga actgtcagaa gaagatagcg aagaggtgcg ggcctcttcc
     1141 gcccgctgtg cctgtgtggt cagtaccaga cggaactcac gcccggcacc gtccgtcacg
     1201 ccggtgattt ccccggccag gtcgcctgcg gcctcacgcc ggtacgtcag cgtccgcccg
     1261 aaacggtctg ccagcccggt aagcacccgg tacggcggca gcggcgctgg cagtacgtcc
     1321 tcagcacccg gcacccgctc tgaccacccc agtatccacc acggcccctg tgcgctgttg
     1381 gtcgccaggt aaagatgcgg gcttaaccgg atatccggcg gcagcgcccc ccacagccgc
     1441 gccagcgtgt ggccgtccgg ctgcgctgcc ttaccaccgc gcaccagcca cattgactcg
     1501 ctgcggctgt acaccgcctc ccccggcagc agcggctcaa agtgaatgct ccgcccgccg
     1561 ttgtcgttga gtaccagtgc atcatcgcgt agctgtaagc ggatatcaga aggcgctttc
     1621 cagccggggc caaacactcc caccggcgcg ggtgtcctgg tccggtagct gctgtaggtg
     1681 cgggagagaa tgaacggcag cgggccgggc agcgcaaggt ccgtctcgcc gggcagcacc
     1741 ttcgccccca gcagcggatt taccgggttg cccgaagtca tcccgcccgg acacaccgag
     1801 cacgccacgc cggtgggcgc gccaattctt acacctgccg aaccctggac aatgggaccg
     1861 ccatactgag tcatatctcc ctgacgcgcc gctggttttc cgctcatata aagtgccctt
     1921 ttagttattg ttcatatccc tgtgttagca acaaaaaaaa taaaatgaca cacaagacca
     1981 gctaatgtat ttatatattt t
//
LOCUS       DAZFWP020000339         1994 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFWP020000339 DAZFWP020000000
VERSION     DAZFWP020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.342"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(33..>1994)
                     /locus_tag="ACHK0Z_RS25925"
                     /old_locus_tag="pgaptmp_005185"
     CDS             complement(33..>1994)
                     /locus_tag="ACHK0Z_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4505014.1"
                     /translation="IEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLIS
                     LVDGSGNPISVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIREND
                     DGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVL
                     ARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTAETTYRFTQLAPGNYRLTVRAVN
                     AWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDPTVQFEFWFSETRIT
                     DIRQVETTARYLGTALYWIAASINIKPDHDYYFYIRSVNTVGKSAFVEAVGQPSDDAS
                     GYLDFFKGQITESHLGKELLEKVDLTEDNASRLEEFSKEWKDANDKWNAMWGVKIEQT
                     KDGKHYVAGIGLSMEDAEEGKLSQFLVAANRITFIDPANGNETPMFVAQGNQIFMNDV
                     FLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKG
                     KLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGA
                     KHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAW
                     LVNNWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          411 a          599 c          497 g          487 t
ORIGIN      
        1 catttctggc gcccgtatat gggttataaa attcagctga tactgatgcc tgcggtggct
       61 ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca gttattcact
      121 agccatgctg ataccgaaaa ctccagcgtc atgtgaccgt gaccggcagg catatcaata
      181 acgccactgt aaatcagcgt attatccagc gcggtacggt tataaatttc agcaccgttt
      241 ttccgcacta tcagacggca tgaggagtaa atatcagtat gctctctctc atgcttagcg
      301 ccactgaatg ccaccgccgg aataacaatc tgccggtcaa acggctgatc gtcataaacc
      361 ctgacggtaa tggtccctga tggccaccgc tccggtgccc gggagtcccg cggaaaagcc
      421 ttacccactg ttttgactat atcgccttca atctggttgg ctgacagttt ccccttaatc
      481 tgacagttct cattaattgt gacattgttg agcgtcccgg cgttcgcatt cacactgcca
      541 ctgatatctg catttttagc ggtcagcttt ccgtccggtg tcagggaaaa tgccggagga
      601 ttaccgccgc tggtaatggt gggagccgtc aggcgcttca ggaacacgtc gttcatgaat
      661 atctggttgc cctgcgccac aaacatcggc gtttcattcc cgtttgccgg gtcaataaat
      721 gtgatacgat tggcggcaac cagaaactgg ctcagtttgc cttcctccgc gtcctccatg
      781 ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg tctgctcaat tttgacgccc
      841 cacatggcat tccacttatc gttggcgtcc ttccactctt tcgaaaactc ctccagtctg
      901 ctggcgttat cctccgtcag gtcgactttt tccagcagct ccttgccgag atgggattcg
      961 gttatctggc ctttgaaaaa atccagatag ccggatgcat catcactcgg ctggccaaca
     1021 gcctccacaa atgccgattt gccaacggtg ttcacactgc ggatgtaaaa ataataatca
     1081 tggtccggtt tgatattgat actggcggct atccagtaca gcgccgtgcc aagatagcgg
     1141 gctgtggttt caacctgcct gatatcggta atccgcgttt ccgagaacca gaactcaaac
     1201 tgtaccgtcg ggtcataaac cgcaagatgc ggcgtggcgg ttatctgaaa atagcccggc
     1261 gtcagctcaa tctgtgacgg cgctgccggt gcggcaatcc tgaacgatac cgatgccgga
     1321 tcgccctgct gcccccacgc atttactgcc cggactgtca gcctgtagtt ccccggcgcc
     1381 agttgtgtga agcggtatgt ggtttccgcc gtccgggctg tgctgaccag ccgctcactg
     1441 ccgtcatccg cggccacggt caggcgaagc atgaaactca cgcccttcac caccttcggc
     1501 gtatcccagc gggccagtac ctgatactcc ccgctgtctg cggtgacttc ggcagtcagg
     1561 tgctgcactg ctggcggcgt gacaccattc accgtgccgc tctggtcgcc gtcaaagtgc
     1621 gccccgttat ccacgatggc ttctttttcc ggtacatgct gcacggcggt gatagcatac
     1681 gtgccgtcat cgttctcacg gatactcaca cagcggaaca ggcgctggcg caacgtcggc
     1741 aacttcagcc cccacacgct gtattcagca acaccgtcag gaacacggct cactttcacc
     1801 ttcacgccgt cggtgacgga ctgaacctcc acgctgatcg gattgccact tccgtcaacc
     1861 aggcttatca gcgtggtacc ggaggatggc agcgtgattt cacggtcgag cgtcagcgtc
     1921 cgggtctggc tgtttaccgc cagcacgcgc ccgccggtgc tgataccggc atagtcatca
     1981 tcacagattt caat
//
LOCUS       DAZFWP020000340         1993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFWP020000340 DAZFWP020000000
VERSION     DAZFWP020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.343"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            206..1537
                     /locus_tag="ACHK0Z_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /pseudo
     CDS             206..1537
                     /locus_tag="ACHK0Z_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1550..1993
                     /locus_tag="ACHK0Z_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /pseudo
     CDS             1550..1993
                     /locus_tag="ACHK0Z_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          654 a          389 c          364 g          586 t
ORIGIN      
        1 tgatgattat taattcacta cagaggaata aaaaataatc tgaagcatac tctgaaacat
       61 cctactctct cactctaata atggcacctt gctcttctta taaagaaggg aataattaag
      121 aatattaaac cttcatcaac ttcacatcaa gaaattaaaa attttaaaca ttaatctaaa
      181 taataacaac ctgatcacat aatcgggtac gattttatcc gataactata atgctataac
      241 tctgggaaat ggttggaata caccgctggg tgccatctct ttttacgctg cccgatcgag
      301 tagtaagtta aataatgata ccagacacga aggtacgagc tatcaggtcg cctataataa
      361 atatctagta caaacagcca ctcgatttag cgtggccgcc tggcgttatg cttcgcagga
      421 ttacaggaca ttcagcgacc atctttacga aaatgataaa cactatcatc agagtgatta
      481 tgatgatttt tatgatattg gcagaaaaaa tagcctttct gctaatatta tgcaaccttt
      541 atccaataat ctgggaaatg tatcattaag tgcgctttgg cggaattact gggggcgaag
      601 tggaaatgct aaagattacc aattcagtta ctccaataac tggcaacaca ttagttatac
      661 tttctctgcc agccaatctt atgatgaaaa taataaagaa gaggagcgtt ttaatctgtt
      721 tatctccatt cctttctact ggggggatga tattgccaaa acacgtcacc aaattaactt
      781 atcgaattcg acctcatttt ccaaagatgg ctattccccc aacaatactg gaattactgg
      841 catagccggt gaacatgatc agttaaatta tggtatatat gttaatcagc aacaacaaaa
      901 taatgatacc tcgcttggta cgaatttaag ctggagaact cccatcgcca taatagatgg
      961 cagctatagt cattctaaaa acgcctggca aagtggtgga agtattagta gtggattagt
     1021 tgtctggccc ggtggtatta atatcactaa ccagttatcc gatacatttg caattctgga
     1081 tgcgtctggg ttagaaggtg cacatattaa tggacaaaaa tacaatcgaa caaacagcaa
     1141 aggccaggtt gtttacgacc tgataatacc tcatcgtgaa aaccatctgg tacttgatat
     1201 agcaaacagt gaaagtgaaa cagaattgca gggcaatcgt caaattattg caccttaccg
     1261 tggagcagtt tcttatgtgc agtttacaac tgaccaacgt aagccctggt atatacaggc
     1321 actgcgtccc gatggttcgc cattaacctt tggctatgac gtactggatc tccaggaaaa
     1381 taatattgga gtcgttggcc agggtagtcg cctttttatt cgcgtagatg aaattcccac
     1441 tggaataaaa gttgctctca atgatgaaca gaacttattc tgtactatta cttttcaaca
     1501 cgttatcgat gaaaacaaaa catatatatg ccagtaaaag gagtttacta tgaagtggct
     1561 tttattaatc accttgagtc tatattcttt tattgtgcaa agtgctccct gtgcattgac
     1621 caatgtcggt gaacaacgtg gaacatatat actcaagcca ctgagtatga aaggtaatct
     1681 aaccgccgaa aaattgaaaa caccggaaat atcattgagt gtacagactt gcaagagaca
     1741 accaacgcca ttaaagttca ttttttaaca cagaacgctt taaggtttaa agtcggaagt
     1801 cgtcattata ttattaattt cccctttgaa tctgacggta aaaaaataga gcttccaggg
     1861 aagtcatctt ataatttaga agaaatgcta tctgcgataa atttcaccat tccatattct
     1921 atcgcatctg tatcagatag ttctaacacc gcagacattt ttcctggaac tccttttcct
     1981 ttgttgttcg aaa
//
LOCUS       DAZFWP020000341         1978 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFWP020000341 DAZFWP020000000
VERSION     DAZFWP020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.344"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..452)
                     /locus_tag="ACHK0Z_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /pseudo
     CDS             complement(1..452)
                     /locus_tag="ACHK0Z_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403105.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            complement(648..1008)
                     /locus_tag="ACHK0Z_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /pseudo
     CDS             complement(648..1008)
                     /locus_tag="ACHK0Z_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1070..1760
                     /locus_tag="ACHK0Z_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /pseudo
     CDS             1070..1760
                     /locus_tag="ACHK0Z_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          592 a          430 c          441 g          515 t
ORIGIN      
        1 acttttgtta tgaaataaac atcagtatcg gcaaagcttg ctgccagcgc ctggttacaa
       61 ccttttcccc ccggtgaaaa tataaaatta cttgccagaa gcgattctcc gatagtggga
      121 agcctcggca aataactgat gatatcaaca ttaaacgaac caataacgca tacttgactt
      181 ttcatagttg agtggccttt ttttattccc taggctgtat cataattttc aagcgcataa
      241 gcgatttcat ttttcgcaat agtatcaagc gagtctagga cgataaaagc accgccatga
      301 tatcgagtca gatagccaag tctggcaagt tcattaagat ctgatcgaat agtttctttc
      361 gttacattga ggctattgga cagatcggat acttttacag aaccgaattt actgagtcgt
      421 ttcaatattt catgatggcg ttcaaatttc atgcttcacc tcacttcagt tacgttaata
      481 tctgtcgatg tatattgatg tgataatcac cataaagtgg cggggaaaag caaaaaaaat
      541 caaaaatagc cggagaaaag gtaagcgtta atggagaatc atattgatgc ttatttattg
      601 gtgagaacta cgttccatgg caggagttca tcaatacggt tggaaggtta ttccggcagt
      661 acgctcagga tatggcgcag atactcttcc ggatcaatgt cgttcagaag gcaagtgccg
      721 ataagcccat acaacagtgc actgcgctcg ccgtcgaaga acataaagtt tttatttccg
      781 agacagatgg cacaaagggc tttttccgcg gcattatgtc tgcctccgcc tgaccgtcaa
      841 cactgtaata acagagtgcg ccccactgat tcagtacaca gctgaaccat tctcccagtc
      901 tggatttttg gacagcgtgg catttttctc caccatccac gcatgcagtg agtccagtaa
      961 cagtttgctc ttcctctgcc tgaccacaag atgttctggt tctggcatac tgaatttggc
     1021 cacctgagca taggtaatat gctcacctta gaacaacaca gatgctccaa tgaaaaaagg
     1081 aaatttcagc gcagagttta aacgcgaatc cactcaacta gttgttaacc agaactttac
     1141 ggtggcaggt accatccaaa gctatggatg tcggtctttc cacaatgaca agataagtca
     1201 aacaactgcg taatgagcat cagagcaaaa caccaaaagc ctctctgatg acaccagaac
     1261 aaatcgaaat acgtgagctg aggaaaaagc ggcaacgtat tgaaatagag aatgaaatat
     1321 tagaaaaggc tacggcgctc ttgatgtcag actccctaaa cagttctcga taatcgagaa
     1381 gctcagagcg cattatctgg tgatagcact ctgccatgtg tgcaggattc atcgaagcag
     1441 ctacagagat tggaaaaacc gtcctgaaaa aaagacggta gacgggctgt attacgtagt
     1501 caggtaattg agcttaatgg cggagcaagg aacatcgcca caattgcaac ccggagagac
     1561 taccagacgg gacgcaagct tgctggcagt ctcatgaaag aacaggggct ggtcagctgt
     1621 cagccgttga ccctccggta caggcatggc agtcatgaac ttgtagctat cctaagaact
     1681 tgaagggcgc ttagctgtga cacagctaaa tcaagtatgg tgacgtgacc tatatctgga
     1741 ctggtaaacg atggggatac ccgcttacgc gagagcaata agtggctggc caccattctc
     1801 gatacaaaac tctgctgaat agcagcgatt ttcccagcgt ggtgcgtctt ttgcatttta
     1861 ctggttctga gttatcaatt atctgttcac taatgactac tctaaaaagt tgtatcaatt
     1921 taagtgatat agggagtccg gagattcacc ctttttggtg atgaagttaa caataccg
//
LOCUS       DAZFWP020000342         1952 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFWP020000342 DAZFWP020000000
VERSION     DAZFWP020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.345"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(23..346)
                     /gene="yecR"
                     /locus_tag="ACHK0Z_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             complement(23..346)
                     /gene="yecR"
                     /locus_tag="ACHK0Z_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU4505015.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            609..695
                     /gene="azuC"
                     /locus_tag="ACHK0Z_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             609..695
                     /gene="azuC"
                     /locus_tag="ACHK0Z_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU4505016.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            809..1060
                     /gene="yecJ"
                     /locus_tag="ACHK0Z_RS25965"
                     /old_locus_tag="pgaptmp_005193"
     CDS             809..1060
                     /gene="yecJ"
                     /locus_tag="ACHK0Z_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU4505017.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1139..1642)
                     /gene="ftnB"
                     /locus_tag="ACHK0Z_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             complement(1139..1642)
                     /gene="ftnB"
                     /locus_tag="ACHK0Z_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU4505018.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT          560 a          414 c          452 g          526 t
ORIGIN      
        1 atcccctcgc tgattaaaat atttagagaa agacagggaa ggcggaatga tggcactgat
       61 atgacaaagt gaattccgta ttcaaacata aggaacccgc aaaaaggcta cagttgccta
      121 ctggctgacc aaaggggacc gcgtgggtat agccttcctg ctggcatgcg cgatcggcga
      181 tcccgcgact gacataacga tcggtatggg catgctgcaa aaaagcctgg tcataaacca
      241 accgtacaat acctgttgcc gcgtcaactt cgctcacatg ggcctggcga gtaaccgtac
      301 accccgccag cgttattaat gaaagagtca gaatcagaag tctcatggtc cagccagaag
      361 agtaagagaa agcactattt tacgcgttac agcgctgcga caatggccgg aaaagacgcc
      421 tggcaggcgc tatttcttgc catctgtagg tgtcagaaat aggggggctg ttttttgatt
      481 atcattttta taacattaaa ggggtgttta tgtgatttgt atcacataaa gattgatttt
      541 tgtgatggtt gtcatattat aagcgcaaag agaataacac ctgtataaca aatggtcgga
      601 gtgccgcgat gaaactgcgc aaaatcctga aaagtatgtt caataactat tgcaagacgt
      661 tcaaagacgt accgccaggc aatatgttcc gataataaaa aacctgctcc ggcaggtttt
      721 ttgtgtcctg atgacggtgg atgtgactat cgctgaagat gtgaagccgc tactataacg
      781 gctttcaaaa tgctatcagg agtttacgat gtcccagccg ctgaatgccg atcaggaact
      841 ggtttctgat gtggttgctt gccagttggt tatcaaacaa attctggatg ttctcgatgt
      901 aatcgcgccc gttgaagttc gtgaaaaaat gtccagtcag ctgaagaata tcgactttac
      961 taaccatccc gccgccgcag acccggtcac catgcgggca atccaaaaag ccatcgcgct
     1021 tattgaactc aagtttaccc cacagggtga atcccattaa aaaaaccgct gcttcaaaca
     1081 gatgaggcag cggtttctca aaaccttcat cggcgcaatg cattagcgcc gatgatgatc
     1141 aatgcagctg gtgtgacacg acattcagaa catgttggtc ggtctgcaca gggcacatac
     1201 ccgcaagttt cgcactgcgc acttcatcaa gaatggtttg cagcaacaga ccatcatgct
     1261 gctgttcttt ttccagatcg cgcaggaaat tgacggttga atcatcattc agttctttcg
     1321 cttcatcggc taactgtgcc aacgtactag aacgttgctc gtattcttcc atcgtttttt
     1381 ggaacagttc ttccagagag ttcagttttt caccgggaac atcaatggct ttaacgatgg
     1441 gggtagcgcc gacactcttc ataaagttaa acatgcgcat catttgggtc acattactct
     1501 gtgcctgggc gcgaaggaaa gtggcggtgc cgttcagact ctgttcagaa caccagttac
     1561 tcaggtgaag gtagagattg gatgcgtaaa actcgcggtt catttgagag ttgagtttga
     1621 gaagcattcc agcggttgcc atacctaata tccttatatc cagaagtgga gaggtgcaag
     1681 ataaatcaaa acaccataat tactgtttgc agaataattc ctttctgccg gtccgaaatg
     1741 tgatcgtgga gtcaattctg acgatgatct gaaataagaa taacgtctga acgggtataa
     1801 gaaaatgctt aaatcatcaa gtttatatat ttttacattt cattgatgaa atcaatgtaa
     1861 ctgcaatgaa ttttaatgat agcgcttttt gtatcttgtt gatattaatc aatgaatttg
     1921 aattcatgat gaagtgatga ttttataaaa cg
//
LOCUS       DAZFWP020000343         1921 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFWP020000343 DAZFWP020000000
VERSION     DAZFWP020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.346"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..315
                     /locus_tag="ACHK0Z_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             <1..315
                     /locus_tag="ACHK0Z_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGU4505019.1"
                     /translation="YQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDS
                     NATRTTDAFMETECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKN
                     VN"
     gene            416..624
                     /locus_tag="ACHK0Z_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /pseudo
     CDS             416..624
                     /locus_tag="ACHK0Z_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            725..1546
                     /locus_tag="ACHK0Z_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             725..1546
                     /locus_tag="ACHK0Z_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4505020.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1546..1746
                     /locus_tag="ACHK0Z_RS25990"
                     /old_locus_tag="pgaptmp_005198"
     CDS             1546..1746
                     /locus_tag="ACHK0Z_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505021.1"
                     /translation="MKHIPEGMIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
BASE COUNT          472 a          511 c          512 g          426 t
ORIGIN      
        1 taccagttcc ggctggataa tggcggaaac gatgaaggat ttggccccct caccatcact
       61 ctgcaactca aagacaaata tggtcagacg ctggtgacca gaaaaatgga aacggaagcc
      121 tttggtgaca gtaatgccac gcgaaccaca gacgcattta tggaaacgga gtgcgtggaa
      181 aatgtcgcca caaccgaaat cattaaagca actgaagaaa gtaacggcca tcgtgtcagt
      241 ctgccgttat cggttttcga tccccaggac taccatccac tgctgattac cgtttccgga
      301 aaaaatgtta actgatccct gaagtcagtt tcatgcttca gcctctgcca caacgtatgg
      361 cagaggctat ttatcaggag aaaccagata tgccgggtta cacacagagt atgtcctggc
      421 tgaaggatct ttctcatacg ggcagacggt ggctgtcaca acagcttacc ttaatgtgct
      481 tattgaagat gaccaggtgc gcacttccgg ctggttatcc gcaacgcgga aggtcagcga
      541 cgctggcgct gccggaatac tgaaccctgt gccggaaagc agcttaatgc gtggctcgcc
      601 agtgaagggc ttctcagaca ataaacgtca ttgatttcgt ccatcaggcc gcgtcttctc
      661 cgggagacgc ggcttttttc atttattaca ccagtaaatc ttaaccaccg ataaggagca
      721 aagtatgcga ttagccagtc gttttggtta tgctgcaaac cagatacgcc gtgaccgtcc
      781 actgacacat gaagagctga tgcaccatgt gccggggatt ttcggggaag ataaacacac
      841 gtcccgaagc cagaactata cgtacatccc caccatcacc gtgctggaaa gtctgcagcg
      901 ggaaggcttc cagccattct tcgcctgcca gacccgtgtg cgcgacccgg gccgtcgggg
      961 atacacaaaa cacatgctgc gtctgcggcg ggccggagag ataaacggag aacatgtccc
     1021 tgaaattatt ctgctcaact ctcatgacgg tacctccagc taccagatgc tgccgggtta
     1081 cttcaggttc gtctgccaga acgggtgcgt ctgtggtcag tctctggggg aagtgcgtgt
     1141 tccgcaccgg ggaaatgtag tggagaaagt tatcgaaggg gcttacgagg tggtgggggt
     1201 ttttgaccgg atagaggaga agcgtgatgc catgcagtcg ctggtcctgc cgccaccggc
     1261 acgccaggcg ctggcacagg cggcactgac ttaccgttat ggtgacgaac atcagcccgt
     1321 caccaccgcc gacattctga cgccacgacg ccgggaggat tacggaaagg acctgtggag
     1381 tgcttatcag accatccagg agaatatgct gaaagggggg atttccggtc gcagtgccag
     1441 aggaaaacgt atccacaccc gtgccattca cagcatcgac accgatatta agctcaaccg
     1501 cgcattgtgg gtaatggcag aaacgctgct ggagagtatg cgctgatgaa acatatccct
     1561 gaaggcatga ttcaggtacc agaatcacct gacgccccgg aggcgctgcc ggggtgtcgt
     1621 tccatttctg atgatgacct gtgcgcctgg tgtacccgtc tgctttaccg tcccggagaa
     1681 ctcagtctgt gcagactgag tacaggcaat ggcatctggc catccgtctg tgaccggaac
     1741 ggttagcgta cggctgtccg gaatttcagc cgaatatcat tcatccctga cattaccggg
     1801 tctcacggtc agtgatgaac gtgatgttgt cagcccccgg cctttactcc ctctcattta
     1861 ttcacattac aaggatttca tacatgaaaa cattgtctca gaacaccacc tcttcggtct
     1921 c
//
LOCUS       DAZFWP020000344         1894 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFWP020000344 DAZFWP020000000
VERSION     DAZFWP020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.347"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            419..634
                     /locus_tag="ACHK0Z_RS25995"
                     /old_locus_tag="pgaptmp_005199"
     CDS             419..634
                     /locus_tag="ACHK0Z_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4505022.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(890..1162)
                     /locus_tag="ACHK0Z_RS26000"
                     /old_locus_tag="pgaptmp_005200"
     CDS             complement(890..1162)
                     /locus_tag="ACHK0Z_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505023.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1322..1855
                     /locus_tag="ACHK0Z_RS26005"
                     /old_locus_tag="pgaptmp_005201"
     CDS             1322..1855
                     /locus_tag="ACHK0Z_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4505024.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          502 a          362 c          502 g          528 t
ORIGIN      
        1 gacctgaaag aagacgctgg ctaaaaggcc ttatatgttt gtagagactt atttttcaca
       61 gacagcaatg atgcctgtca atatattatc aatatgcgga ttgtttcagt tacagatgct
      121 ttattaagga aaaaaacagc cagcactgac tttcggtgga gaggtgctgg ctcagaagga
      181 tagttggatt tcacatgata cttatgcctg gcggtatatt ttctgacaga cagtgacggg
      241 tgttgtcaag atattgtgtc atttataacc tgaatcaggg gaggccggaa tgttatctgg
      301 catttttagc agagcctgaa tgccataatc acggctcccg gagttggccg tcagtgggtg
      361 acactggcgg tttttttgtt tttctttact ttcattttct gtcggcggtg acggagacat
      421 acatcagatg gaaaaaatca caacaggtgt gtcatacacc acgtcagcgg tggggacggg
      481 atactggtta ctgcagctgc tggacaaagt ctctccgtcc cagtgggtgg cgataggtgt
      541 gctggggagt ctgctgtttg gcctgctgac gtatcttact aacctgtatt tcaaaatcag
      601 agaggaccgt cgtaaggcgg cgcggggaga gtagtcgatg aataaacaat acgaactggt
      661 tgtaaaatga atatttctaa ctgaaaaaac gttccatgag gtgagaaaag gtcacaggca
      721 atcaataaca ggacgtgatg aaagaccctt gcatttgtgc gctttctctt tagatagcag
      781 cagatactga aaatctgagt tgtcggggag tcagggatac agctgtgcaa gagttggtca
      841 ttgtgattcc attgaaatcc tgtatgccat gaagggcagg attttatggc tacctgagct
      901 ttggtgatag taagttgaaa attcgcattt tttgctgaca tgcgtaacga gaatcccata
      961 agcagggagg acttaattct tcattaaccc atgcgttgat attatgtttc agccgttgaa
     1021 gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt tttatgtgag atcttaccgt
     1081 aagggtctgc aagaatgctg cttgttgctt cgttattatc tgccatcaga agaagtaact
     1141 ctgatttaac gttttctgtc attagttgta aaaatcttct gcgcaaactt tctttactgt
     1201 tcatttatat ggcttcattt gttgtaatct gctgcgtctc aagggatatg tttatgagag
     1261 cgaccatgag tgttggatta tatacctaac atatcaaggg attagaaatc gataaatccc
     1321 catgaacgaa aaaataaaat acggcctgtc ggctgccgtt ctggcgctga ttggtgcagg
     1381 tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa aaggaaggta accacaccac
     1441 ggcataccgt gatggtgcgg ggatctggac catctgccgt ggtgccattc tggtggatgg
     1501 taagcctgtt attcctggca tgaagctgtc aaaggaaaaa tgcgaccggg ttaatgccat
     1561 cgaacgtgac aaggcgctgg catgggtgga gaaaaacatc aaagtgccgc tgaccgaacc
     1621 ccagaaagcg gggatcgcgt cattctgtcc gtataacatt ggccccggta agtgtttccc
     1681 gtcgacgttt tataaacgaa ttaatgcagg tgatcgaaaa ggtgcctgcg aagcgattcg
     1741 ctggtggatt aaggacggtg gcagagactg ccgtatccgc tcaaataatt gctacggtca
     1801 ggtatcccgg cgagatcagg aaagtgcgct ggcgtgctgg ggtatcgaca gataagcaga
     1861 atattttgct taaaaatgac gttggccaac gcgg
//
LOCUS       DAZFWP020000345         1889 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFWP020000345 DAZFWP020000000
VERSION     DAZFWP020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.348"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..139)
                     /locus_tag="ACHK0Z_RS26010"
                     /old_locus_tag="pgaptmp_005202"
     CDS             complement(<1..139)
                     /locus_tag="ACHK0Z_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4505025.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHARTNAPG
                     SD"
     gene            complement(207..>1889)
                     /locus_tag="ACHK0Z_RS26015"
                     /old_locus_tag="pgaptmp_005203"
     CDS             complement(207..>1889)
                     /locus_tag="ACHK0Z_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4505026.1"
                     /translation="IVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWD
                     TPRVVKGVRFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPLGEYTLTVRAINSYGQQ
                     GEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQV
                     ETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGL
                     FREKIGKLHLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSATIQQ
                     IQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMI
                     NPESGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADIS
                     GNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTIT
                     VRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLI
                     YSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          395 a          554 c          477 g          463 t
ORIGIN      
        1 tatcgctacc gggagcgttc gtccgggcat gaagataccc cgcgctcagc gtggcctgct
       61 gctcagacgc ccatgcaggc gcaccggata cggtcagaca aatggctgcg gacaaaatgg
      121 cggcataaag tttacgcata attacctctc gcttttctgc aataaaaaaa ggcgtcattt
      181 ctgacgcccg ttctgggtta taaaattcag ctgatactga tacctgctgt ggattttttc
      241 atcaccacaa ccagcagatc gctgatactt gctgtgggat accagttatt taccagccat
      301 gctgacaccg aaaactccag cgtcatgtga ccgtgaccgg caggcatatc aataacgcca
      361 ctgtaaatca gcgtattatc cagcgcggta cggttataaa tttcagcacc gtttttccgc
      421 actatcagac ggcatgagga gtaaatatca gtatgctctc tctcatgttt agcgccgctg
      481 aatgccaccg ccggaataac aatttgccgg tcaaacggct gatcgtcata aaccctgacg
      541 gtaatggtcc ctgatggcca ccgctccggt gcacgggaat cccgggggaa agctttgccc
      601 actgttttaa cgagatcgcc ttcaatctgg ttcgcggaca gttttcccag aacccgacag
      661 ttctcgttaa tcgtgacgtt gttgagcgtc ccggaattcg cattcacgtt accgctgata
      721 tcagcatttc ttgcggtcag cctgccctcc ggcgtcaggg aaaacgtcgg gggattgccg
      781 gacgaggtga tactcaccgc aaacagtcgc ttcaggaaca catcgttcat gaacaactga
      841 ttcccctgcg ccacaaataa cggcgtggtg ttgccgctct ccgggttaat catcgcaata
      901 cggtcagcca gcagcagtat gttgctcagg ggctggccat cagtatcctc aatccccgct
      961 ccaataccgg caacataggg tatgccattt tttgttttct gtaccttcag catgtaaagt
     1021 gcagcaaggt catcatttgt gtccttctgc acgcgctgta tctgctgtat ggtggcgctc
     1081 tggtcctcca gcgttttact gaccgtctgt gtgatttcat tgcgggtttc ggtgatggag
     1141 gtcttcatct ccgccatctc atccgcaagc tggctgttgt ctatcagctc ccacagcccc
     1201 tgagccagat gcagttttcc tattttttcc cgaaacagcc ccagataccc ttctgcatca
     1261 ttgctggccc ggccactggc ttccacaaaa gcagatttcc ccaccaggtt gacgctgcgc
     1321 acgtaaaacc agaaatcctt cccgggctta atgtgcgggc cggatacact ccactgactg
     1381 ccggtcccca gataacgggc agaggtttcc acctgagatg tgtctgcgat ttttgtctcc
     1441 gaaaaccaga actcaaactg taccgtcggg tcatacaccg caagacgcgg gaccgccgtt
     1501 atctgaaaat accccggcgt cagttcaatg gtggcgggtt ttgctggcgc gttaatccgg
     1561 aaggtggtgg tggccggttc gccctgctgg ccataactgt taattgccct gactgtcagg
     1621 gtgtattccc cgagcggcag accactggaa cgatgctctg tatccgcggt gatggcggtg
     1681 gtcaccagac ggctgccttc tccgcttccg ctggtcagtc gcagactgaa gcgcaccccc
     1741 ttcaccaccc gcggcgtgtc ccatttcgcc tgtgccagat actggccgtc agctgcgctc
     1801 acctccaccg tcaggtgctg cactgccgga gggatgacgc tgttcagggt gcctgactgc
     1861 ggctcaaagc tggccccgtt atccacgat
//
LOCUS       DAZFWP020000346         1879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFWP020000346 DAZFWP020000000
VERSION     DAZFWP020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.349"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            130..384
                     /gene="bssS"
                     /locus_tag="ACHK0Z_RS26020"
                     /old_locus_tag="pgaptmp_005204"
     CDS             130..384
                     /gene="bssS"
                     /locus_tag="ACHK0Z_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU4505027.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            499..1617
                     /gene="solA"
                     /locus_tag="ACHK0Z_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             499..1617
                     /gene="solA"
                     /locus_tag="ACHK0Z_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU4505028.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1665..1778
                     /locus_tag="ACHK0Z_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             1665..1778
                     /locus_tag="ACHK0Z_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU4505029.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          485 a          455 c          444 g          495 t
ORIGIN      
        1 aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga
       61 agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag
      121 ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac
      181 atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat
      241 aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac tgatgttgcc
      301 agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttcccg
      361 gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg
      421 gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat
      481 gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc
      541 gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat
      601 atgccaccgc atcaacacgg cagccaccat ggcgatacgc gattaattcg ccatgcttat
      661 ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg ggatgaactt
      721 tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg
      781 gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt
      841 gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac
      901 tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc
      961 tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca
     1021 attcgtcatg acgatgatgg cgtaactatt gaaaccgttg acggtgagta tcaggcgaaa
     1081 aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag
     1141 cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa
     1201 ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa
     1261 aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa
     1321 cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt cttgcgcaat
     1381 gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct
     1441 gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct cattaccggc
     1501 ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg
     1561 caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca
     1621 tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc
     1681 actatctcat caataatatt cgcgagcatc tgatgcttta tctttttctc tggggattgt
     1741 tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg acataaactg
     1801 acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgagatta ttcctgcttc
     1861 tacagcactc accagacat
//
LOCUS       DAZFWP020000347         1879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFWP020000347 DAZFWP020000000
VERSION     DAZFWP020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.350"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            119..466
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS26035"
                     /old_locus_tag="pgaptmp_005207"
     CDS             119..466
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4505030.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            516..>1879
                     /locus_tag="ACHK0Z_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             516..>1879
                     /locus_tag="ACHK0Z_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HGU4505031.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQTPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTGRLWAYVRDDRNAGS
                     ALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHARR
                     KIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWL
                     REKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFL
                     FFGS"
BASE COUNT          419 a          522 c          567 g          371 t
ORIGIN      
        1 cagaggaccc ggaaaccctc agtatcagct gtgaggtaac gttccggcac gggacgctcc
       61 gcttcaatgg caatgtcagc gaaaagctcc tgactctgct gatacaggaa ctgaagcgat
      121 gatcccgtta ccttccggga ccaaaatttg gctggttgcc ggtatcaccg atatgagaaa
      181 tggcttcaac ggcctggctg cgaaagtaca aacggcgctg aaagacgatc ccatgtccgg
      241 ccatgttttc attttccggg gccgcagcgg cagtcaggtt aaactgctgt ggtccaccgg
      301 tgacggactg tgcctcctga ccaaacggct ggagcgtggg cgcttcgcct ggccgtcagc
      361 ccgtgatggc aaagtgttcc ttacgcaggc gcagctggcg atgctgctgg aaggtatcga
      421 ctggcgacag cctaagcggc tgctgacctc cctgaccatg ctgtaaatct ctttatcctg
      481 gttgtcacag aataagcccg gtaaaatacg ggcttatgaa cgacatctct tctgacgaca
      541 tcttcctgct gaaacagcgc ctggccgaac aggaagcgct gatccacgcc ctgcaggaaa
      601 agctgagcaa ccgggagcgc gaaatagacc atctgcaggc gcagctggat aaactccgcc
      661 ggatgaactt cggcagtcgt tccgaaaaag tctcccgccg tatcgcacaa atggaagccg
      721 atctgaaccg gcttcagaaa gagagcgata cgctgactgg tagggtgtat gacccggcag
      781 tacagcgtcc gttgcgtcag acccgcaccc gtaagccgtt ccctgaatca ctaccccgtg
      841 acgaaaagcg actgttgcct gcggcgccgt gctgcccgaa ctgcggcggt tcactgagct
      901 atctgggcga ggataccgcc gaacagctgg agttgatgcg tagcgcattc cgggttatcc
      961 ggacggtacg ggaaaaacat gcctgtactc agtgcgatgc catcgtgcag acacctgcac
     1021 cttcgcggcc catcgagcgg ggtatcgccg gaccggggct gctggcccgc gtgctgacct
     1081 cgaagtatgc agagcacacc ccgctgtatc gccagtcaga aatatacggc cggcaaggtg
     1141 tggagctgag gcgttcactg ctgtcgggct gggtggatgc atgctgccgg ctgctgtctc
     1201 cgctggaaga ggcgcttcat ggctatgtca tgactgacgg caaactccat gccgatgata
     1261 ccccggtcca ggtactgctg ccgggtaata agaagaccgg gcggttgtgg gcgtatgttc
     1321 gtgatgaccg caatgcaggg tcagcgttgg cacctgcagt gtggttcgct tacagcccgg
     1381 acagaaaagg catccatccg cagactcatc ttgcctgctt cagcggtgtg ctgcaagcgg
     1441 atgcgtacgc cgggttcaac gagctgtatc gcaatggtgg gataacggaa gctgcctgct
     1501 gggctcatgc ccgccgaaag atccacgatg tgcacgtccg catcccgtca gcactgacgg
     1561 aagaagccct ggagcagatc ggtcagttgt acgccataga ggcggatata aggggaatgc
     1621 cggcagagca gcggcttgct gaacgtcagc gaaaaacgaa accgttgttg aaatccctgg
     1681 aaagctggtt gcgtgaaaag atgaagaccc tgtcgcgaca ctcagagttg gcgaaggcgt
     1741 tcgcgtacgc acttaaccag tggccggcac tgacgtacta tgcgaacgat ggctgggtgg
     1801 aaatcgacaa caacatcgct gaaaatgccc tgcgggcggt cagtctgggt cgtaaaaact
     1861 tcctgttctt cggctctga
//
LOCUS       DAZFWP020000348         1867 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFWP020000348 DAZFWP020000000
VERSION     DAZFWP020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.351"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            27..728
                     /locus_tag="ACHK0Z_RS26045"
                     /old_locus_tag="pgaptmp_005209"
     CDS             27..728
                     /locus_tag="ACHK0Z_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4505032.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            725..925
                     /locus_tag="ACHK0Z_RS26050"
                     /old_locus_tag="pgaptmp_005210"
     CDS             725..925
                     /locus_tag="ACHK0Z_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505033.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            1124..1305
                     /locus_tag="ACHK0Z_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /pseudo
     CDS             1124..1305
                     /locus_tag="ACHK0Z_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1311..>1867
                     /locus_tag="ACHK0Z_RS26060"
                     /old_locus_tag="pgaptmp_005212"
     CDS             1311..>1867
                     /locus_tag="ACHK0Z_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505034.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHG"
BASE COUNT          600 a          392 c          409 g          466 t
ORIGIN      
        1 tcaacttaga attgcatggt gatgatatgg aagccttaca aacaacacca aaaacatgta
       61 gcctcaatac ttacaacaag gttttatgtg atgatttaga tctcgattct tttgcattaa
      121 ccatcgcaaa cctgctcagt gctgttcgca ccttcaacct cctagatgat acgcgattaa
      181 aagaggttgg gtttgatgtg ctagaattta ctcatgaata tgctttagcg atcgcatcga
      241 caaaacaaca acattctatc cgatcaggca acaagatagc ctgcatacgc accaaacgtg
      301 aagcctgcgg cttgacgact accgaactag ccagactact cgatcttgac gaagagatta
      361 ttctgcaatg ggagagtgga gagttcgagc caaccatcag catgcttatc cccctggcaa
      421 acgtcctggg atgcgatccg ctttctctac taagtgaaaa aaacagcgag tcagctattc
      481 gtgtaaatac gactgaagtc catgcggaaa gtattggcgc acgcatcaaa agcgcccgta
      541 caaagctggg attaaccgaa tctgatcttg cccgcatgat tcatacctac agtgacccca
      601 taaacgactg ggaatgcggc atctgtgaag ttcctgctga tcatatagta ccactggcca
      661 gtgcgcttaa ttgtgacctg atgtggttgt taacgggaaa atcagaagca aaggagcagc
      721 aacaatgact ggcaatatcc atgataagta tgaaggctta tgcctggcac cggattcctt
      781 tgcaaacaat atccataatt tattatgcgc agttatcgta ttacaaatgt cagacaacga
      841 cgcaataaaa agaacaggtg atgaagttct tgaatttgca cgttgctatg ctgaagcagc
      901 agctgaaaaa gaactatcca gttaaataga acaagtcatc tccagatact atattacggc
      961 ttaatcgccg gggattatca cacacttaat ccacaggagg ttttattatg acttttataa
     1021 aacataaggc atcacacaaa acagcctgcc ttattgcaca acacgggaaa aatcacatgc
     1081 atattgcctg cttgtttctg cgtaaagcat acgggagata ataatgcatc agaaaacagc
     1141 agaacacgaa caaaccagag tattgctgac catcaaaaac gggaaagtaa tattaattcg
     1201 tcatgttcat gacgatgaat ttgtagaaat ctttcaacat tcctgtttat tgcagaaaag
     1261 gcaggatatg acgttattgc accagcagat gaagatgagg aataaatttc atgcaatacg
     1321 ctgaattcca ggctgaagca acagccaatg gtatacaaac aggcagtatg actattgatt
     1381 atcacgacgc catacgccgt ctggatgctg gagaattcga tactcctaat gtgcgaggtt
     1441 tacgtatcct tcagtgtctc gcgcaagctg acgaagcagg attactggga aaacttccgg
     1501 ttgagatgaa ggttgcccag tggcgatggt tgtatgtgac gacattcatc aacgaagaag
     1561 aagacaagaa cggcacaatt gatgtcccga atgaacacgg aacaacagat cgcgccgtaa
     1621 tatataacgg gaagcatggg gttatgacga tatatcccgg ccccattcgg tttgccttac
     1681 agcagtatat tgaatggaat ttaattcaaa aatacggcga ggcagaagga atgggtagag
     1741 cgctgtttct ttatcagaaa atgctcatta catacccaga taaaggtttc attgtttcag
     1801 acatggggcg agaagggctt gaactccttc tggatgaaat gattaacgac ctgaatactc
     1861 atggtat
//
LOCUS       DAZFWP020000349         1863 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFWP020000349 DAZFWP020000000
VERSION     DAZFWP020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.352"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1192
                     /locus_tag="ACHK0Z_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             <1..1192
                     /locus_tag="ACHK0Z_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4505035.1"
                     /translation="QYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSN
                     GRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALK
                     DPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPARVVYLTAGIDSQRNR
                     FEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYCHADGTEMTISRVCW
                     DTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAK
                     EILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWD
                     NKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVN
                     GYSR"
     gene            1176..1382
                     /locus_tag="ACHK0Z_RS26070"
                     /old_locus_tag="pgaptmp_005214"
     CDS             1176..1382
                     /locus_tag="ACHK0Z_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU4505036.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTVASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            1379..>1863
                     /locus_tag="ACHK0Z_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     CDS             1379..>1863
                     /locus_tag="ACHK0Z_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4505037.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEEKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     L"
BASE COUNT          434 a          431 c          599 g          399 t
ORIGIN      
        1 gcagtatctg aaatttggcg atgagtccac gccttttggg cttaaatggg agaaggacag
       61 ccccgaaagt gttttctacc tctgtgaaca tcatggctgc gtgatccatc agtctgaact
      121 ggaccagagc aacgggcggt ggatctgtga aaacacgggc atgtggaccc gtgacggtct
      181 gacgtttttc agcgcccggg gtgatgaaat tccgccgccg cgctccatca cgttccatat
      241 ctggacggcg tacagtccgt ttaccacctg ggtacagata gtctatgact ggctggatgc
      301 actgaaagat cccaacggcc tgaaaacctt tgtgaacacc acgctgggcg agacctggga
      361 agaagccgtg ggcgaaaaac tcgatcacca ggtactgatg gataaggtcg tgcattacac
      421 ggcggcggta cctgcccggg tggtttatct gacggcgggc attgactcgc agcgaaaccg
      481 ttttgagatg tatgtctggg gatgggcacc gggagaggaa gcttttctgg tggataaaat
      541 catcattatg ggccgtcccg atgaggaaga gacgctgtta cgtgtggatg cggcgatcaa
      601 caaaaaatac tgccatgcag acggaaccga aatgaccatt tcccgtgtct gctgggacac
      661 cggggggatc gatggtgaaa ttgtctatca gaggtcaaaa aaacacggtg ttttccgggt
      721 gctgccggta aaaggcgcat ctgtctatgg caagccggtg atcaccatgc cgaaaacccg
      781 caatcagcgg ggcgtgtatc tgtgtgaagt ggggacggac accgcaaaag aaattctcta
      841 tgcccgtatg aaagccgatc ccactcctgc ggatgaagcc acgtcgtatg ccatccgttt
      901 tcctgatgat ccggagattt tttcgcagac agaggcgcag caactggtcg cggaagagct
      961 tgtggagaag tgggaaaaag gaaagatgcg tctgctgtgg gataacaaaa agcggcgtaa
     1021 cgaagcgctg gactgcctgg tgtatgccta cgcggcatta cgtgtgtccg tgcaacgctg
     1081 gcagcttgat ctggctgtac tggcaaaatc ccgggaagaa gagacgaccc ggccaaccct
     1141 gaaagaactg gcagcgaagc tgtccggagg agtgaatggt tacagtcgct gaactacagg
     1201 cgctacgtca ggcgcgcctt gatttattaa ccggtaaacg ggtggtgtct gtccagaaag
     1261 atggtcgcag aattgaatat acggtggctt ctctggatga gcttaaccgg gcgatcaatg
     1321 atgcggagtc ggtactgggg acaacccgac gtcgccgtcg tccgctggga gtgaggttat
     1381 gaaacgaacg cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta
     1441 caacgggggc ggcgcaggat ttggcgggca aatggcggag tggttgccac cggcgcagag
     1501 tgccgatgca gccctgctgc ctgcgttgcg tctggggaat gcccgggcag atgatctggt
     1561 gcgcaataac gggatagcgg ccaatgcggt ggccctgcat aaggatcata ttgtcgggca
     1621 tatgtttctg atcagctacc gtccgaactg gcgctggctg gggatgcggg agactgcggc
     1681 aaaaagtttt gtcgatgagg tggaggcggc ctggtcggaa tacgccgaag ggatgtctgg
     1741 cgagatcgac gtggaagaga aacgcacgtt tacggaattt attcgtgaag gtgtgggcgt
     1801 tcatgcgttt aacggcgaaa tctttgtgca gccggtctgg gatacggaga gcacgcaact
     1861 gtt
//
LOCUS       DAZFWP020000350         1863 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFWP020000350 DAZFWP020000000
VERSION     DAZFWP020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.353"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1192
                     /locus_tag="ACHK0Z_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             <1..1192
                     /locus_tag="ACHK0Z_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4505038.1"
                     /translation="QYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSN
                     GRWICENTGMWTRDGLTFFSARGDEIPPPRSIMFHIWTAYSPFTTWVQIVYDWLDALK
                     DPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPARVVYLTAGIDSQRNR
                     FEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCW
                     DTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAK
                     EILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWD
                     NKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVN
                     GYSR"
     gene            1176..1382
                     /locus_tag="ACHK0Z_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             1176..1382
                     /locus_tag="ACHK0Z_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU4505039.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            1379..>1863
                     /locus_tag="ACHK0Z_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             1379..>1863
                     /locus_tag="ACHK0Z_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4505040.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSVDA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     L"
BASE COUNT          430 a          429 c          604 g          400 t
ORIGIN      
        1 gcagtatctg aaatttggcg atgagtccac gccttttggg cttaaatggg agaaggacag
       61 ccctgaaagt gttttctacc tctgtgaaca tcatggctgc gtgatccatc agtctgagct
      121 tgaccagagc aacgggcggt ggatctgtga aaacacgggc atgtggaccc gtgacggtct
      181 gacgtttttc agcgcccggg gtgatgaaat tccgccgccg cgctccatca tgttccatat
      241 ctggacggcg tacagtccgt tcaccacctg ggtacagatt gtctatgact ggctggatgc
      301 actgaaagat cccaacggcc tgaaaacctt tgtgaacacc acgctgggcg agacctggga
      361 agaggccgtg ggcgaaaaac tcgatcacca ggtactgatg gataaggtgg tgcgttacac
      421 ggcggcggtg cctgcccggg tggtttatct gacggcgggc attgactcgc agcgaaaccg
      481 ttttgagatg tatgtctggg gatgggctcc gggagaggaa gcctttctgg tggataaaat
      541 catcattatg gggcgtcctg atgaggaaga gacgctgtta cgtgtggatg cggcgatcaa
      601 caaaaaatac cgccatgcgg atggcaccga aatgactatt tcccgtgtct gctgggacac
      661 cggggggatc gatggtgaaa ttgtttatca gagatcaaaa aaacacggtg ttttccgggt
      721 gctgccggta aaaggcgcat ctgtctatgg caagccggtg atcaccatgc caaaaacccg
      781 caatcagcgg ggcgtgtatc tgtgtgaagt gggaacggat accgcaaaag aaattctcta
      841 tgcccgtatg aaagccgatc ccacgcctgc ggatgaagcc acgtcgtatg ccatccgttt
      901 tcctgatgat ccggagattt tttcgcagac agaggcgcag caactggtgg cggaagagct
      961 ggtggagaag tgggaaaaag gaaagatgcg tctgctgtgg gataacaaaa agcggcgtaa
     1021 cgaagcgctg gactgcctgg tgtatgccta cgcggcatta cgtgtgtccg tgcaacgctg
     1081 gcagcttgat ctggctgtac tggcaaaatc ccgggaagaa gagacgaccc ggccaaccct
     1141 gaaagaactg gcagcgaagc tgtccggagg agtgaatggt tacagtcgct gaactgcagg
     1201 cgctgcgtca ggcgcgcctt gatttattaa ccggtaaacg ggtggtgtct gtccagaaag
     1261 atggtcgcag aattgaatat acggcggctt ctctggatga gcttaaccgg gcgatcaatg
     1321 atgcggagtc ggtactgggg acaacccgac gtcgccgtcg tccgctggga gtgaggttat
     1381 gaaacgaacg cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta
     1441 caacgggggc ggcgcaggat ttggcgggca aatggcggag tggttgccac cggcgcagag
     1501 tgtcgatgcg gccctgctgc ccgcgttgcg tctggggaat gcccgggcag atgatctggt
     1561 gcgcaataac ggaatagcgg ctaatgcggt ggccctgcat aaggatcaca ttgtcgggca
     1621 tatgtttctg atcagctacc gtccgaactg gcgctggctg gggatgcggg agaccgcagc
     1681 aaaaagcttt gtcgatgagg tggaggcggc ctggtcggaa tacgcagaag ggatgtctgg
     1741 cgagatcgac gtggaaggaa aacgcacgtt cacggaattt atccgtgaag gtgtgggcgt
     1801 tcatgcgttt aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt
     1861 att
//
LOCUS       DAZFWP020000351         1859 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFWP020000351 DAZFWP020000000
VERSION     DAZFWP020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.354"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..242)
                     /locus_tag="ACHK0Z_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     CDS             complement(<1..242)
                     /locus_tag="ACHK0Z_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505041.1"
                     /translation="MAIKGLDQTIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVNRKLVKERSRLKRATVRNPNARIIVNRGDLP"
     gene            complement(257..610)
                     /locus_tag="ACHK0Z_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             complement(257..610)
                     /locus_tag="ACHK0Z_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4505042.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(603..977)
                     /gene="gpFI"
                     /locus_tag="ACHK0Z_RS26105"
                     /old_locus_tag="pgaptmp_005221"
     CDS             complement(603..977)
                     /gene="gpFI"
                     /locus_tag="ACHK0Z_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4505043.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEIQS
                     SGEDVQVGDTVIRERETAAHDVREDTSGALTRIRVLTCLHLCGIDGETGESVEIADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1029..>1859)
                     /locus_tag="ACHK0Z_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             complement(1029..>1859)
                     /locus_tag="ACHK0Z_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4505044.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEIQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNITQGSGK
                     EWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPEGMLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
BASE COUNT          429 a          587 c          414 g          429 t
ORIGIN      
        1 gggagatcac cgcggttaac gataattctg gcattcggat ttctgaccgt ggcccgtttc
       61 agtctggacc gttcctttac cagtttccgg ttcaccctgg tttcccgggc aacctgtgac
      121 gaagactgat taatcgccgt tgtggccacg cggttaatgg ccattgctga agccgccgga
      181 atggcgtttt tacgaacccg gctcagattt tcaatcgtct gatcaagccc ttttatcgcc
      241 ataattcacc ctgcgtttat cgtcgccggt taacggcggg tggttgacca cggttgagcc
      301 agagataaca gctgcccccg tcatccggag aaacacgatc cacccagaat atctcaccat
      361 taatggtcag cgtgtcacca cgccgcacgg cacgaaccgt atccgtccgc acaaataatg
      421 acgggctgct tccttcaata cggaccccgc caccggcaaa ccccagcgac tccggatcgt
      481 caaaaacccc ctgaacttcg ctgccacact gtgcccccga ggtgaactgc gcacagagcc
      541 ccatcacttc aacaatcgtg ctgtccaccc cggcaagggc agcatcaaag gcattctgaa
      601 aatcacgcat aaacagccat tccgccatca acgtgtgttt ttgcatctga ggacataatc
      661 agaatcaccc gaccaacatc cgcaatctca acggattccc ctgtttcacc atcaatgcca
      721 cagagatgga ggcaggtcag aactctgatg cgcgttaacg caccggatgt atcctcacga
      781 acatcatgag ccgcggtttc ccgctcccgg atcaccgtat ccccaacctg aacatcctcg
      841 ccggatgact gtatttcctc ttcccattcc gccacccgct gcgctatctc tgcggcactc
      901 ccggatatat ccggctcacg ccccagaatc agggccagtt catcaagccg tttcagattt
      961 tgctctttcg ttgccatatc agccccctgt gaaaaaagac acgggggcat ttcgcccccg
     1021 ctcacggatt atttcacctg taccaccaca aactcatccg ggtccggcaa caccatcagc
     1081 ggcgcggact gcgtcatggt aaattcacgg gcgggatccc ccaccgtcag ccagtgtttc
     1141 ggataacggg aagaggccac cacaccttcg gacaacgcct gcgcatcctg aatggcaccg
     1201 taacaacgga tcccatctgc agcagtattc cccagaacca gcatgccctc cggcagataa
     1261 cgtttttcga taccgtcttc tgctatataa gacgttttcg ccaccacaat ggccagatcg
     1321 ccgtaatacc ccttgaagga caccactgcg cccagatctt tcactgccgt ttcgagttga
     1381 gaatttgaac cgcgacgggt atccagtttt tcgcggaaca gcttaaaacc attcagaaga
     1441 cgccagacgg taccgtccat aatggcaata ttcacaagac cgctggcctg gtcgcagtag
     1501 aggtcaagat catgtgtagg atcgaacgtg tcacgatcct gttttgacca ctccttacca
     1561 ctaccctgag tgatgttatt cttcgtcgac ctgccaaaat cgacctcaat tttctcgaac
     1621 tggtctcctt ccatcgtata tttgccatac aacacagcat ttaccgcctg tatttcttcc
     1681 acctggacaa tcgcgtgctc ttcctgtttg aggttatcgg taatgatacg cagacggcgg
     1741 taagccggat cgttcagttg agccggatct tcaccaggaa ggcgctcaac cgcctgctgg
     1801 taattaaatt cgtgtttcgg cttgacgtag cccggacgta acacgcgggt ttcaccacc
//
LOCUS       DAZFWP020000352         1828 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFWP020000352 DAZFWP020000000
VERSION     DAZFWP020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.355"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..755)
                     /locus_tag="ACHK0Z_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             complement(<1..755)
                     /locus_tag="ACHK0Z_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4505045.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLD"
     gene            complement(851..1039)
                     /locus_tag="ACHK0Z_RS26120"
                     /old_locus_tag="pgaptmp_005224"
     CDS             complement(851..1039)
                     /locus_tag="ACHK0Z_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4505046.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            complement(1036..1224)
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             complement(1036..1224)
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4505047.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            1624..>1828
                     /locus_tag="ACHK0Z_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             1624..>1828
                     /locus_tag="ACHK0Z_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505048.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQYRILIRVRRTTTRDSVLV
                     SIFNPEPQWRIKCQTKKEIRLLKN"
BASE COUNT          463 a          444 c          411 g          510 t
ORIGIN      
        1 tcatccagcg ttttccatgt ctgcttgtat tcgcgacaaa tggcggcagt gactgggttg
       61 atttttcctg ctgagttttc agtgttctgt tgattgactc tggcgctggc gagatcaaca
      121 acagacgtgt attttccagt ctctttgcgc tctgcgtcct gccgtttttt ccagttacgt
      181 aattcagcct gaatttcggg ccatttggca cccggattac atttgtgttt aacccatccg
      241 atagcgaaca gtttgcgttc cggatacata gcgttaattt caggcgtttt catcagtgct
      301 tcaacgatat gcccgtcaaa ggtagcaacg tcttcctgca gtaattcctg cgcgtcaatc
      361 gccatatcaa cggtgatgtt ttcacatgta ccgaacttaa ccaggaccgc gttctgtact
      421 tcaagggaca gcttgtcaaa attgacgttc atcggatcgg attctggttc gaccggaata
      481 aaggaagcgg attcctcatc ccagcggttt tcctgcatat attcagcatc ccaggaatcg
      541 agggcagggc ggggtatacc gggtttatcc tcgcagacaa gaaatttata agcgcagtcc
      601 tgagcagccg gataatgttc caggaattgc cagtgaaatt ttgcgcgggc gcgacgttca
      661 tcaccggctt caatggcagt ggctacagcg acggcacctt cttcctttat tgcctgttcg
      721 tccggaatgg cggcgcaaat aaagacttta ctcattttgt tttaacctca ttacagattt
      781 cagggtgaac gaatccctgc cattgctggc atttttaatc cgttggtatg gcgttaatat
      841 ggctggcggg ttatccagcc ggtatttcgt tattcaggtt cagcgatact ttttttaacg
      901 ggaggcattc accggggatt ttttgttcgt cccttacctg aatgcaggat gacttactgt
      961 cataaattcc ggtaatcaca ttttgtggct cacccgttat aagaaaaacg gtcatcacca
     1021 gtgcaaatgc tgaagtcact gctgttctcc gataatacca agttcaagaa gggcaattct
     1081 ggaaagtatg gaattatcat tgagaagata aggttcatat tttctcatct taatggcatc
     1141 ttccgtaaac tcccggttac tgagcagaac accaatatca aaacaacctt cagacgtatt
     1201 aacgtttggt aataacgttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg
     1261 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagaatccac
     1321 ccataacact gtttttggtt ttaactgttc cgcgtgcgct cagccgcatt caccacatca
     1381 caaaattcac tttaaaaagg gcggcagagc agtcacggag taaaactgat accgccaaac
     1441 gtcaccagaa aattgataac agagggcgtt gcagcggggt tgtcacttaa gcgtatggtc
     1501 aacctgacaa cccggtgtcc tcaacgggga aggaataacc cctccatact taccgccgcg
     1561 ccatttcgcg gattgccaca accggaagcg cacggtcgac gaaaatttaa cgacaggcta
     1621 tctatgaacc agctacctcg ccgtgcgctt tcgcgttatg gtctgacttt tcagggaaat
     1681 atcctttcag taaactgtca gtaccggatt cttatccgtg tccggcgcac gaccacacgt
     1741 gacagcgtgt tggtctccat ttttaaccca gaacctcaat ggaggataaa atgccaaaca
     1801 aaaaaagaaa tccgcttatt gaaaaaca
//
LOCUS       DAZFWP020000353         1817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFWP020000353 DAZFWP020000000
VERSION     DAZFWP020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.356"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1817
                     /locus_tag="ACHK0Z_RS26135"
                     /old_locus_tag="pgaptmp_005227"
     CDS             <1..>1817
                     /locus_tag="ACHK0Z_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4505049.1"
                     /translation="QFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSGLMAMARQ
                     FRNVTAEQIAYVAQLQRSGDEAGALQAANDIATKGFDEQTRRLKENMGTLETWADKTG
                     KAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQ
                     GAWNDRENARLGLAAATLQSDMEKAGELAARDRAERDASQLKYTGEAQKGYERLLTPL
                     EKYTARQEELNKALKDGKILQADYNTLMAAAKKDYESTLKKPKSSGVKVSAGERQEDQ
                     AHAALLALETELRTLEKHSGANEKISQQRRDLWKAENQYAVLKEAATKRQLSEQEKSL
                     LTHEKETLEYKRQLAELGDKVEHQKRLNELAQQAARFEQQQGAKQAAISAQARGLTDR
                     QAQRESEEQHLRDVYGDNPDALAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESA
                     TDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVG
                     SIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTK
                     EATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASG"
BASE COUNT          441 a          446 c          619 g          311 t
ORIGIN      
        1 acagtttgat gccatcaacc agagtgtcgc gcgttttgct tctgcatccg gtgtggaggt
       61 ggacaaggtt gcagaggctt tcggaaaact gaccaccgac ccgacgtcgg ggctgatggc
      121 gatggcgcgc cagttccgta acgtgacggc agagcagatt gcgtatgttg cgcagctgca
      181 gcgttccggt gatgaggccg gggccttaca ggcggcgaac gatatcgcca cgaaaggctt
      241 tgatgagcag acccgtcgcc tgaaagaaaa catggggaca ctggagacct gggcggataa
      301 aacagggaag gcattcaaat cgatgtggga tgccattctg gatatcggtc gtcctgagtc
      361 ctcagcggat atgctcgcca gtgcgcagaa ggcatttgat gaggcggata aaaaatggca
      421 gtggtaccag agccggagcc agcgccgcgg taaaacctct tctttccggg ccaaccttca
      481 gggcgcatgg aatgaccggg aaaatgcccg gctggggctg gcagcggcca cgctgcagtc
      541 ggatatggaa aaagccggtg aactggccgc cagggaccgg gccgaacggg acgcatcaca
      601 gctgaagtat accggagagg cgcagaaggg gtatgagcgt ctgctgacgc cgctggagaa
      661 atataccgcc cgtcaggaag aactgaataa ggccctgaaa gacgggaaaa tcctgcaggc
      721 ggattacaac acgctgatgg cggcggcgaa aaaggattat gaatcgacgc tgaaaaagcc
      781 gaagtcgtca ggagtcaaag tgtcagccgg tgagcgtcag gaagaccagg cgcatgctgc
      841 cctgctggcg cttgaaaccg agctccggac gctggaaaaa cacagcggtg cgaatgagaa
      901 aatcagccag cagcgtcgcg atttatggaa agcggaaaat cagtatgcgg tcctgaaaga
      961 ggcagccacg aaacggcagt tatctgagca ggaaaaatcc ctgctgaccc atgagaaaga
     1021 gacgctggag tacaaacgcc agctggctga gctgggagac aaagttgaac accagaaacg
     1081 gctgaatgag ctggcacagc aggctgcgcg gtttgagcag cagcagggcg cgaagcaggc
     1141 ggcaatcagt gcccaggcgc ggggcctcac cgaccgtcag gcgcagcggg agtcggaaga
     1201 gcagcacctt cgtgacgtgt acggtgataa tccggatgcg ctggcgaagg ccacatctgc
     1261 actgaagaac acctggtctg cggaggagca gcttcgtgga agctggatgg ccggtctgaa
     1321 gtccggctgg ggcgagtggg cagaaagtgc gacggacagt ttttcgcagg ttaaaagcgt
     1381 ggccacgcag acctttgacg gtattgcaca gaatatggca gcgatgctga ccggcagtga
     1441 gcagaactgg cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa
     1501 gcaggcaatg gtggggatag tcgggagtat cggtagcgcc attggcgggg ctgttggtgg
     1561 cggcgcatcc gcatcaggcg gtacagccat tcaggcagct gcggcgaaat tccattttgc
     1621 gaccggagga tttacgggaa ccggcggcaa atatgagcca gcggggattg ttcaccgtgg
     1681 tgagtttgtc ttcacgaagg aggcaaccag ccggattggc gtggggaatc tttaccggct
     1741 tatgcgcggc tatgccaccg gcggttatgt cggtacaccg ggcagtctgg cggacagccg
     1801 gtcgcaggcg tccggga
//
LOCUS       DAZFWP020000354         1814 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFWP020000354 DAZFWP020000000
VERSION     DAZFWP020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.357"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1189..1791
                     /gene="fimB"
                     /locus_tag="ACHK0Z_RS26140"
                     /old_locus_tag="pgaptmp_005228"
     CDS             1189..1791
                     /gene="fimB"
                     /locus_tag="ACHK0Z_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU4505050.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          545 a          326 c          387 g          556 t
ORIGIN      
        1 ccattttacc cagagtcata aaatagaatg tctgttgatc tcattttcat attcctttca
       61 gactgaagtg cgttttcatc acccgatgag aaagaactga agtgatgttt gccatagatt
      121 gcgagatgcc ctgtctcatt gactatcttg gtgcaccttt atacctgtta taccagatca
      181 aaaatcacgc aatccataca acaaaaccag atttgcaatt cgtgtcacaa aatatgtcga
      241 tatttttcta agaagaagat gccatgtgaa gccagacgaa cacttgcgga ggccttcaca
      301 aaccaaagat tgttgtttaa ttatctgttt tataagtaat ttatttaaag tataaacaag
      361 gaaagatttg caagttaact cagtgagcgt ggtgaaaatc agtgtttacc cgccatcagg
      421 ctgagcataa ttctcatcat gaaatatgtt tcctggtttg tggcttgtaa ctggtcactt
      481 ctgaagtcga tctggagagg cttgttgatg ttggtgtttt caggatgatg tttcacttag
      541 tttgtttgcc gtatcgcccg gcgaatggct gtgattgagg aaggtttaag tcgtagtgac
      601 caaagctata tttaccaacg aatgtagatg aaaaaatcat ctcctgcgtt ccccatatct
      661 ccaggataaa aaggaatgta acaatctcat ggcgtaagct gacgaatcag ccaggaataa
      721 tcgctaggga cctaagaatt agcatgataa tagccactaa gaaattactg cgctccatga
      781 aatagccatt ttgtggcaaa tggagttgac taataatgtc atatgtgaga cggctagttg
      841 aacgaatatt aaattttgct gaatttttta tgttgatttt acttgttaca gaacatatca
      901 catgatatat agataagatt agttgcatta atgatgaggg ttattattag attcgtatcc
      961 gattgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaacgtt
     1021 attataatta actgttgcta ctccatttaa gttcactcag aagaactggt ccacttacgt
     1081 tagttattaa gcaaacgttc gcttttataa acataatcag gataaaaatg ttggattatt
     1141 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaaacgat gaagaataag
     1201 gctgataaca aaaaaaggaa cttcctgacc catagtgaaa tcgaatcact ccttaaagca
     1261 gcaaataccg ggcctcatgc agcacgtaat tattgtctga ctttgctttg ttttattcat
     1321 ggtttccggg cgagtgaaat ttgtcgattg aggatttcgg atattgatct taaggcaaag
     1381 tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa cgcatccgct attgaacaaa
     1441 gaagttcagg ctttaaaaaa ctggttgagt atccgtactt cgtacccgca tgctgagagc
     1501 gagtgggtat ttttatctcg taagggaaat ccgctttctc ggcaacagtt ttaccatatt
     1561 atctcgactt ccggtggtaa tgccgggttg tcactggaga ttcatccgca catgttacgc
     1621 cattcgtgtg gttttgcttt ggcgaatatg ggaatagata cgcgacttat ccaggattat
     1681 cttgggcatc gcaatattcg tcatactgtc tggtataccg ccagcaatgc agggcgtttt
     1741 tacggcatct gggatagagc cagaggacga cagcgtcacg ctgttttata gagatagttg
     1801 agataccagg gatg
//
LOCUS       DAZFWP020000355         1752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFWP020000355 DAZFWP020000000
VERSION     DAZFWP020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.358"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            93..365
                     /locus_tag="ACHK0Z_RS26145"
                     /old_locus_tag="pgaptmp_005229"
     CDS             93..365
                     /locus_tag="ACHK0Z_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505051.1"
                     /translation="MAQHIKSHNSEAVPAIKRGRHSHVFKTDWYQHPSCTEEQAEWLI
                     QCYRRRGYEVKKAISLDYRHWIISVRLPYSEHPPRPSRTFQQRIWR"
     gene            367..1416
                     /locus_tag="ACHK0Z_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             367..1416
                     /locus_tag="ACHK0Z_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4505052.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHCGRVLVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFCDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARCSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKCRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPQEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            1429..>1752
                     /locus_tag="ACHK0Z_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             1429..>1752
                     /locus_tag="ACHK0Z_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4505053.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVRGQ
                     LVPGGR"
BASE COUNT          410 a          452 c          482 g          408 t
ORIGIN      
        1 tttcggcaga ctttgttttt tcctggcatt ctggtttaca attcgcacgt cagcctgaac
       61 aactggcacc tgctgcgtca ccggagaacc cgatggcgca acatataaaa tcccacaatt
      121 ctgaagccgt cccggccatt aagcgggggc ggcattcgca cgtatttaaa accgactggt
      181 accagcatcc ctcctgcact gaagaacagg ccgaatggct gattcagtgt taccgcaggc
      241 gcggatacga ggttaagaaa gccatcagcc tcgattatcg tcactggata atctctgtca
      301 ggctccctta ttccgaacat ccaccgcgtc cgtcccgcac attccagcaa cgtatctgga
      361 ggtaacgtgc gggtattact tcgacctgtt ctggtgccgg aacttggcct ggtggtcctt
      421 aagcccggac gcgaatcact gcctgttttt cattgcggca gggtgctggt ggagccggaa
      481 ccgaaaaaca tgcgcggtct gccgtccgga gtcgttcctg ccgttcgcca gccgctggca
      541 gaggataaaa cattactgcc atttttctgc gatgagcggg ttattcgtgc agcaggtggt
      601 gcaggtgcac tgtctgactg gttattacgt cacgtgaaat cctgccagtg gccacacggc
      661 gattatcatc acagcgaaac cgtcattcac cgttatggta ccggcgcgat ggtgttgtgc
      721 tggcactgtg acaaccagct gcgcgaccag acatcagaat cactcgatca acttgctcag
      781 cagaatctgg ttgcctggat gattgacgtc atccgtcacg caataagcgg tacgcaggag
      841 agggagttat cgctggccga attatcctgg tgggcggcct gcaatcaggt ggtggatgca
      901 ctacctgagg cagtagcgcg ttgttcgctg ggattaccag cggaaaaaat ccgctccgta
      961 taccgtgaga gtgacatcgt accgggagaa cagacagcca tcagcatact gaagcagcgc
     1021 acaaaaaata ttgcgctgcc acttcacgtc caccagcaac aaaatccacc acagaaaaaa
     1081 acggttgtca gtatcgccgt tgatccggag tctcctgaat cgttcatgag gcggcctaaa
     1141 tgtcgccgtt gggttaatga gaaatacacg cgctgggtaa agacacagcc gtgtgcgtgt
     1201 tgtggtaagc cagctgacga tccgcatcac ctgattggtc atggtcaggg gggaatggga
     1261 acaaaggccc acgatatttt cacgctaccg ttgtgccggg agcaccacaa cgaacttcat
     1321 gcagacccgc aggagtttga gaaaaagtac ggctctcaga ttgagttaat ttttcgtttt
     1381 cttgatcacg cctttgcgac tggcgtgctc gggtaaaaga ggtgactgat gctcatagat
     1441 ttggttttac cttacccgcc gacggtgaac acttactggc gacgccgtgg cagcacatat
     1501 tttgtatcaa aagccgggga gcgttatcgc cgggcagtgg cgcttattgt tcgccagcag
     1561 cggctgaaat taagcctgtc cggacggctg gcaataaaaa ttattgcaga gccaccggat
     1621 aagcgtcgtc gtgacctgga caacattctg aaagcaccgc tggatgcgct gacgcatgcg
     1681 ggagtgctca ttgatgacga gcagtttgat gaaatcaata ttgtacgtgg tcagcttgtt
     1741 cctggtggtc gg
//
LOCUS       DAZFWP020000356         1749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFWP020000356 DAZFWP020000000
VERSION     DAZFWP020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.359"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..1103
                     /gene="yiaK"
                     /locus_tag="ACHK0Z_RS26160"
                     /old_locus_tag="pgaptmp_005232"
     CDS             105..1103
                     /gene="yiaK"
                     /locus_tag="ACHK0Z_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGU4505054.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1115..1582
                     /locus_tag="ACHK0Z_RS26165"
                     /old_locus_tag="pgaptmp_005233"
     CDS             1115..1582
                     /locus_tag="ACHK0Z_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGU4505055.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT          472 a          418 c          428 g          431 t
ORIGIN      
        1 caatcagatt gctttgttaa aaagcgatcg atatatttga aatcaagttt cgcatattga
       61 aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc tgggatgaaa gtgacatttg
      121 agcagttaaa agcagccttt aatcgggtct taatttcacg cggcgttgac agcgaaacgg
      181 ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg
      241 ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt cctgatgccc
      301 aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggatgcccag cgttcgatcg
      361 gcaacctgac ggcgaaaaag atgatggatc gcgccattga actggctgcc gatcacggta
      421 ttggtctggt ggcacttcgt aatgccaacc actggatgcg cggcggcagc tacggctggc
      481 aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc gtaatgccgc
      541 cgtggggcgc aaaagagtgt cgcattggca ctaacccgct gatcgtcgcc attccttcca
      601 cgccgatcac catggtcgat atgtcgatgt cgatgttctc ttacggcatg ttagaagtta
      661 accgcctggc gggccgtcag ctcccggtcg atggtggctt tgatgatgag ggcaatttga
      721 ccaaagaacc tggcgttatc gagaagaatc gccgcatttt gccgatgggc tactggaaag
      781 gttctggcat gtcgattgtg ctggatatga tcgctactct cctttccgac ggcgcatcgg
      841 ttgccgaagt cacccaggac aacagcgacg aatacggcat ttcacaaatt tttattgcca
      901 ttgaagtgga caagcttatc gacggtccca cccgcgatgc caagctgcaa cgtatcatgg
      961 attacgttac taccgctgaa cgcgctgacg aaaatcaggc catccgctta cccggccacg
     1021 aatttactac cctgctggcc gaaaaccgcc gtaacggtat taccgttgat gacagcgtgt
     1081 gggccaaaat ccaggcgtta taaggagata agtcatgatt tttggacata tcgcgcagcc
     1141 caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc gcgctaccga
     1201 tttcaacgcc ctggagccgg gcgttgtcga aatcgacggc aaaaatattt atgcgcaaat
     1261 tatcgattta accacacgcg aagcggtgga aaaccgtccg gaagtgcatc gtcggtatat
     1321 cgatattcag tttctggcgt ggggtgaaga gaaaatcggc attgctattg atacgggaaa
     1381 taataaagtc agcgaatcac tattagagca gcgcgatatt attttttatc atgacagtga
     1441 acatgaatca tttatagaaa tgataccggg cagctacgca attttctttc cgcaggatgt
     1501 tcaccgacca ggttgtattt tgcaaacagc ctctgagata cgaaaaattg tggttaaagt
     1561 cgcattaacg gcgctgaatt aatcgaataa tcgtctacgc cagaacgact gggtaatgtt
     1621 attgctctaa ctatattaat aatttaggtt gctgcacatg caacttgaat tatgacagac
     1681 atagcctcaa ggaatagcta tgaaaaaaat acttgaagca atactggcga ttaatctcgc
     1741 cgtactttc
//
LOCUS       DAZFWP020000357         1747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFWP020000357 DAZFWP020000000
VERSION     DAZFWP020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.360"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(178..318)
                     /locus_tag="ACHK0Z_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             complement(178..318)
                     /locus_tag="ACHK0Z_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505056.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(675..899)
                     /locus_tag="ACHK0Z_RS26175"
                     /old_locus_tag="pgaptmp_005235"
     CDS             complement(675..899)
                     /locus_tag="ACHK0Z_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505057.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(964..1170)
                     /locus_tag="ACHK0Z_RS26180"
                     /old_locus_tag="pgaptmp_005236"
     CDS             complement(964..1170)
                     /locus_tag="ACHK0Z_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4505058.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            complement(1393..1524)
                     /locus_tag="ACHK0Z_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             complement(1393..1524)
                     /locus_tag="ACHK0Z_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505059.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLG"
     gene            complement(1548..>1747)
                     /locus_tag="ACHK0Z_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             complement(1548..>1747)
                     /locus_tag="ACHK0Z_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505060.1"
                     /translation="TQVNKSKNPVCWLQTGFYVYGSKLWEAALIDFSKLIRELRLMIS
                     QLPNWKFLLVWSIPFLWVVSQ"
BASE COUNT          523 a          441 c          365 g          418 t
ORIGIN      
        1 agaaggctgt caacagagcg ttcgggtatg aattacataa ttaagtaaca tggtgttcac
       61 agaacacaca gttaccggac acatcagttt tccattcgtc ccccggcagt acaggcttcc
      121 cgtctgacgg gatagcctga aaaaacacag aaaattattt gttataatta atataactta
      181 ctcaaaaaaa agcgacgaga aaatcagcat caacgagcaa taagcgccaa tacgtgataa
      241 caaatggcag ccatatttat ctgcagtata agcaatggac aggataacca caccagaaac
      301 cgtcagcata aaatccattt gaacttcccc ggacaaaatc gactcatcta aagatttaca
      361 gctcttttta ttatcaatat gttaaaagta aaataaacag atgttcaata atacgaatac
      421 aaaaacgtgc tgaaaatcaa tgaatccatt tctgtgttat caattaatag tgataaacat
      481 ccggcttctt ccaccatcgc accggaccag cgaccatgag gggacaacgc cgcgctccgt
      541 taacgcggta aaccccggtg tgtatcgttt ttgattatcc ccgcacactc gcgcagagga
      601 ttctcccggt cgggctgcgg tctctgttaa tgcaggaata cggcgacaat accgcgcatg
      661 aataataagg tcgctcaaca cactggctgt aatgcagcgg ataccatgcg gcatttagcg
      721 gcattcatcg tacactcaac ggttagctct tcattcgtgg cattcacctg aaaggtccgg
      781 gagtgtaatt gcgtacattt accactgaac gaaccttcaa caagaacacg accacgctgc
      841 aaaatacgga acggaattgt tccctgaaaa ggctttacgg ttaccaataa tttcttcatg
      901 cattctccga ataacaaaaa tactagttaa tacactgagt gcggatatat tcctgaagca
      961 ttctcaatgc tgcctggtcg ctgatgattc cgtctctgat accgagaacg tttcgtccag
     1021 caaccggaga gagttcgacg gcggcatcat tgcccacgcc ggaggtgccg gtggcttcac
     1081 gcacggtacc ggggcaggtg gcgttgatcc gcaggcgctt acgaccagcg gcaacatcag
     1141 cacgcagagt ttcattttca gctctcgcat cggctaattc cctcgagtat tttgcatcga
     1201 gcgcagcaac atcgcgctgg cgcacctgca tatcagtaat ggttgcgttt gccagatcca
     1261 gctcactggc ttttttatcg cgctgcgctt tgtaggtgat ggcgttatcg cggtaatgat
     1321 tcagccccag actaagcgca ccacagacca ccagcagaat aacggtaaac gcggaaagca
     1381 ttcggtttat gctcacccca gcagccccga cgaagacaac atcatccagc ccatggaaag
     1441 aaaaagagca accagcatta gtgaaaatga aatgccgacg attacacaga ggatcttcgc
     1501 cagcattatg agtttgtctg acatagctac cccttaattg ccacaattca ctgggatact
     1561 acccataaaa aagggatgct ccagaccagc aaaaatttcc agtttggtaa ttgactaatc
     1621 atgagtcgca actccctaat cagtttgcta aaatcaatca aggcagcctc ccatagctta
     1681 ctgccataaa cataaaaccc cgtttgcaac caacaaacgg ggtttttact tttattcact
     1741 tgggttt
//
LOCUS       DAZFWP020000358         1741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFWP020000358 DAZFWP020000000
VERSION     DAZFWP020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.361"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            26..133
                     /locus_tag="ACHK0Z_RS26195"
                     /old_locus_tag="pgaptmp_005239"
     CDS             26..133
                     /locus_tag="ACHK0Z_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505061.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            339..1622
                     /gene="gltA"
                     /locus_tag="ACHK0Z_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             339..1622
                     /gene="gltA"
                     /locus_tag="ACHK0Z_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU4505062.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          462 a          418 c          402 g          459 t
ORIGIN      
        1 caaacattac caggaaaagc atataatgcg taaaagttat gaagtcggta tttcacctaa
       61 gattaactta tgtaacagtg tggaagtatt gaccaattca ttcgggacag ttattagtgg
      121 tagacaagtt taataattcg gattgctaag tacttgattc gccatttatt cgtcatcaat
      181 ggatccttta cctgcaagcg cccagagctc tgtacccagg ttttcccctc tttcacagag
      241 cggcgagcca aataacaaac gggtaaagcc aggttgatgt gcgaaggcaa atttaagttc
      301 cggcagtctt acgtaataag gcgctaagga gaccttaaat ggctgataca aaagcaaaac
      361 tcaccctcaa cggggacaca gctgttgaac tggatgtgct gaaaggcacg ctgggtcaag
      421 atgttattga tatccgtact ctcggttcaa aaggtgtgtt cacctttgac ccaggcttca
      481 cttcaaccgc atcctgcgaa tctaaaatta cttttattga tggtgatgaa ggtattttgc
      541 tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc taactacctg gaagtttgtt
      601 acatcctgct gaatggtgaa aaaccgactc aggaacagta tgacgaattt aaaactacgg
      661 tgacccgtca taccatgatc cacgagcaga ttacccgtct gttccacgct ttccgtcgcg
      721 actcacatcc aatggcagtc atgtgtggta ttaccggcgc gctggcggcg ttctatcacg
      781 actcgctgga tgttaacaat cctcgtcatc gtgaaattgc cgcgttccgc ctgctgtcga
      841 aaatgccgac catggccgcg atgtgttaca agtattccat tggtcagcca tttgtttatc
      901 cgcgcaacga tctctcctat gccggtaact tcctgaatat gatgttctcc acgccgtgcg
      961 aaccgtatga agttaatccg attctggaac gtgctatgga ccgtattctg atcctgcacg
     1021 ctgaccatga acagaacgcc tctacctcca ccgtgcgtac cgctggctct tcgggtgcga
     1081 acccgtttgc ctgtatcgca gcaggtattg cttcactgtg gggacctgcg cacggtggtg
     1141 ctaacgaagc ggcgctgaaa atgctggaag aaattagctc cgttaaacac attccggaat
     1201 ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct gatgggcttc ggtcaccgcg
     1261 tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga aacctgccat gaagttctga
     1321 aagagctggg caccaaagat gacctgctgg aagtggctat ggagctggaa aacatcgcgc
     1381 tgaacgaccc gtactttatc gagaagaaac tgtacccgaa cgtcgatttc tactctggta
     1441 tcatcctgaa agcgatgggt attccgtctt ccatgttcac cgtcattttc gcaatggcac
     1501 gtaccgttgg ctggatcgcc cactggagcg aaatgcacag tgacggtatg aagattgccc
     1561 gtccgcgtca gctgtataca ggatatgaaa aacgcgactt taaaagcgat atcaagcgtt
     1621 aatggttgat tgctaagttg taaatatttt aacccgccgt tcaaatggcg ggttaatttt
     1681 tatatgccta aacacaaaaa attgtaaaaa taaaatccac tatcggacat tcatatattc
     1741 t
//
LOCUS       DAZFWP020000359         1721 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFWP020000359 DAZFWP020000000
VERSION     DAZFWP020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.362"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            80..718
                     /locus_tag="ACHK0Z_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             80..718
                     /locus_tag="ACHK0Z_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4505063.1"
                     /translation="MPVVLNISDGRALPESELEALRNIARSNQSDTLIVGSRSLRLHH
                     ISFMDAFSVEPIQGSLLDRLGGRCRRLAESLERQLNHGNTFLQAFSLYMEQVRLTPFW
                     LEGGRNAVQTRINSHAFTVNPGDFPCCEQHLSCPITLCIPKTGVFVKNALHSKVCSLY
                     DKDALSEAIRHNVFHPLSREAFSPEMIVGREECYFDLTDQRFCIISGQDVRF"
     gene            1009..>1721
                     /locus_tag="ACHK0Z_RS26210"
                     /old_locus_tag="pgaptmp_005242"
     CDS             1009..>1721
                     /locus_tag="ACHK0Z_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
                     /protein_id="HGU4505064.1"
                     /translation="MLSTSQLKLSGTACHHITETPASFKSEITSRQLEDARESGCLHI
                     KNCDMEFLPHLPEEITAITIENCNKLTGLTGLPVNIKSLSVINCENLKVSELPPTVRE
                     LHIELNNSSFVHTVPEGVEHLKLGHCQVSGVPDSVRSLELTGRAAETLTGVPSGLCSL
                     SIRDYNPEYQVRIDNFISPSLQTLSLTGCSNIVLPEKLPKGMVSATIYTEQKTTWNIV
                     AEGFPEEMDLDLQNVQLSP"
BASE COUNT          452 a          321 c          421 g          527 t
ORIGIN      
        1 tttgtctgtt ttattttatg tgtggatgtt tatttgttgt tggcctggat aagctaaata
       61 cattacagag ggagataata tgcccgtcgt attaaatatc tcagatggaa gggcgctgcc
      121 tgaaagtgaa ctggaagctc tgcgtaatat agcaagaagc aatcagagtg atactctgat
      181 tgtgggaagt cgtagtctga ggctgcacca tatttcattt atggatgctt ttagtgtgga
      241 gccgattcag ggcagtcttc ttgaccgtct tggagggagg tgtcgtcgcc ttgcagagag
      301 tctggagaga cagcttaatc atggcaatac tttcctgcag gcgttcagtt tatatatgga
      361 gcaggttcgt ttaaccccat tctggttgga agggggcaga aatgcagtgc agaccagaat
      421 taattctcat gcatttaccg tcaatcctgg agattttcct tgctgtgagc aacatctgag
      481 ttgcccgata acattatgta taccaaaaac aggcgttttt gttaaaaatg cactgcattc
      541 aaaagtctgt tctttatacg ataaggatgc cttgtctgag gctatccgac acaatgtttt
      601 tcatcccctc agtcgtgaag cgttttctcc agaaatgatc gttggcagag aggagtgtta
      661 ttttgatcta actgatcagc gtttctgcat catttccggg caggatgtac gtttttgagt
      721 gggggatatt aatacagact gagttgtact gactgctgct ttttattcgt cttcgattga
      781 caacattatt ttgctggttg ttagaatccg ggtcagattg ctgggtttat tctggaatga
      841 acatgtagta actgtacttg cttttccagc gtgaaatagc gtctgtaatg atgccttttc
      901 gccatcggga aaccctggat acaggctctc tgtatgaaat ttgtcaggta agctcctcgg
      961 atggggctta ccttatagtg atttattttc tgaaaatgga atttattgat gctttccaca
     1021 tcacaattaa aattgtcagg gactgcctgt catcatatta ctgaaacacc tgcaagcttt
     1081 aaaagtgaaa ttacgtcccg gcaactggaa gatgcacggg aaagtggttg tctgcatata
     1141 aaaaattgtg acatggagtt cttgcctcat ttaccggaag aaatcacagc gataacaata
     1201 gagaactgta ataagctgac aggtcttaca gggctaccgg tcaatataaa aagcctgtcc
     1261 gttattaatt gtgagaatct gaaagtcagt gaactgccgc cgacagtaag agagctacat
     1321 attgaattga ataattcgtc ttttgtacat actgttccag agggcgttga gcatttgaaa
     1381 cttggtcact gtcaggtatc gggtgtgcct gatagtgtcc ggtctcttga gttgacgggt
     1441 agggctgcgg agacgcttac cggcgtacca tcagggttgt gttctctgag tatccgggat
     1501 tataacccgg agtatcaggt cagaatcgat aattttatat ccccttcact gcagacgttg
     1561 tctctgacgg gttgcagtaa tatcgtgttg ccggaaaaac tcccaaaagg gatggtgtca
     1621 gcaaccattt atacagagca gaaaacgaca tggaacatcg ttgctgaagg atttcccgaa
     1681 gagatggatc ttgatttaca gaatgtacag ctttctccag a
//
LOCUS       DAZFWP020000360         1717 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFWP020000360 DAZFWP020000000
VERSION     DAZFWP020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.363"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(5..202)
                     /locus_tag="ACHK0Z_RS26215"
                     /old_locus_tag="pgaptmp_005243"
     CDS             complement(5..202)
                     /locus_tag="ACHK0Z_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGU4505065.1"
                     /translation="MIGKAIIKAQYNKQVPLTLVAMSVLTAMSIACQNQVDVCSPGNL
                     RGAVNIYTMVLEKGKRPGERY"
     gene            complement(429..725)
                     /locus_tag="ACHK0Z_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             complement(429..725)
                     /locus_tag="ACHK0Z_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4505066.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            complement(904..1640)
                     /locus_tag="ACHK0Z_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /pseudo
     CDS             complement(904..1640)
                     /locus_tag="ACHK0Z_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          464 a          366 c          366 g          521 t
ORIGIN      
        1 ttcactaata cctttccccc ggtcttttcc ctttctccag aaccatagtg taaatattaa
       61 cggccccacg taaatttccg ggactgcata cgtcaacctg attctgacag gcaattgaca
      121 tcgctgttaa tactgacatt gcaaccagag tcagaggtac ttgtttattg tattgagctt
      181 taattattgc cttccctatc attgatggtc atgcatatac tgaatacgac atatccggtt
      241 tgatgtaagg cataaatgct tcctttttcg taatacgttt cggttatagc agcaattacc
      301 gctacaatag cgacaatagc tgctctttca acaatgaatg ctggaaattc tgtttatcaa
      361 ttaaacttct ggctaaattg ttcttatgct tctttttcct cctgaggtga tcacgtgatg
      421 acaaaaactt atctgctcat ggtcatatct gaagaaagcg tatgtctcac ttcgtcatta
      481 acacctttga ctgctgatct ggcaggtaca gtatgtaaag taagccattc gtcaatagct
      541 gatgaacgcc agcccacaga accactacca agacgtaccg gcctggggaa ggttgcatcg
      601 tagtatttcg ataacggatt cattttttcg taaattgtcg aacgagatat acctaacaat
      661 tggctcagtt caggcatccg taagatgcgt gaaggagtcc tgcagtgtcc ggattgatct
      721 ggcattttat cccccatagt gtggtgaact gtcgttacag tttgtattcc ccgggtaaat
      781 ttgtctgcgt aaaaaaatgg gttgagaaaa aagacaataa ataattaaaa atcaatatga
      841 tagaaaacga cagcattagt gttattttac ttttccctta tcgctgtcga ttctcattat
      901 cattcatggc aaaaaaacag caaaagaggc cctggtaatg actcctgtca gtagtacatg
      961 tgttttcgat aaatgaacca atggcttttc gtgaatttcc agggagcgta aaaagcaaat
     1021 cgttttccgg gcaggtcgtt tgatgtgagt acgaagtgtc agattgttgc gctcaatgcg
     1081 ccgggtaaat atcttgccga caagatgctt atcctgtggc atttctctgg tataactgct
     1141 ccggttgtct ctggttatca tgcctgcgga gaatggcttc aggaattccg gcaactcacg
     1201 gcatgtttca tcagtgcgag gaccaaaagt gtaagccagc acaccgtcag ctttggtctt
     1261 atacgcgtac cagtgccact gctgacgagc tttgttttcg acaaaactcc attgttcatc
     1321 aagttcacag ataagtgcca catcgacact ggcggctgga gatatggtta ctttacgtgg
     1381 tgagcgtttt ttaaagtgag aatgacggtg ttgatatcca ccttcagcgt tctggagcta
     1441 tcgcgaaccc cggctccgtt atgaaccatt tcaacaattt gctctttaat gcctggtttt
     1501 cgggtttcgt agatgtaatt cagttgaaat acacgtttgc aggattagca ctggaaacgc
     1561 tcatgatctg aggtgctgtg atcataacgg tacactttat aggaattaca gcggggagaa
     1621 tatactgtaa ctgttgccat atggtctcca gataccaata gaatacaaca ttaatctatc
     1681 gtcagaaggc atcaccgggg ccttctgaca taatctg
//
LOCUS       DAZFWP020000361         1705 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFWP020000361 DAZFWP020000000
VERSION     DAZFWP020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.364"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(97..540)
                     /locus_tag="ACHK0Z_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             complement(97..540)
                     /locus_tag="ACHK0Z_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4505067.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            697..1626
                     /gene="metA"
                     /locus_tag="ACHK0Z_RS26235"
                     /old_locus_tag="pgaptmp_005247"
     CDS             697..1626
                     /gene="metA"
                     /locus_tag="ACHK0Z_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU4505068.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          407 a          475 c          389 g          434 t
ORIGIN      
        1 agtcggaaga ctgggccttt ttgcgctggt gcagaaaaag gtcggatgcg acgctggcgc
       61 gtcttctcca gcctgggcag gcacggcagg ggcggcttat tcccccacat acgccagatt
      121 cagcaacgga tacggtttcc ccaaatcgtc cacctcagag cgtcccgtaa ccttaaaacc
      181 caccttctta tagaacccaa ccgcctgctc attttgctca ttaacgttgg ttgtcagttc
      241 cggtgccatc gagagcgcat gctccaccag catccgaccc acgccgcagc cgcgcacatc
      301 aggatcgata aacagcgcat ccatatgctg cccacttaac aacataaacc caaccggctg
      361 atcccgttca ttaaccgcga cccacaacgg tgcttccggc agaaaggaac gaaccagctc
      421 ttccagctcg gcccgatact ctgctgataa aaaatcgtgg gtggcatcga cagatcgaca
      481 ccaaatcgca acgagttcct ccccttcctc atgctgtgag cggcgaatac taataaccat
      541 tttctctcct tttcgtcatt cttatattct aacgtagcct tttccttgaa actttctcac
      601 cttcaacatg caggctcgac attggcaaat tttctggtta tcttcagcta tctggatgtc
      661 taaacgtata agcgtatgta gtgaggtaat caggttatgc ctattcgtgt gccggacgag
      721 ctacccgccg tcaatttctt gcgtgaagaa aacgtctttg tgatgacaac ttctcgtgcg
      781 tctggtcagg aaattcgtcc acttaaggtt ctgatcctta acctgatgcc gaagaagatt
      841 gaaactgaaa atcagtttct gcgcctgctt tcaaactcac ctttgcaggt cgatattcag
      901 ctgttgcgca tcgattcccg tgaatcgcgc aacacgcccg cagagcatct gaacaacttc
      961 tactgtaact ttgaagatat tcaggaacag aactttgacg gtctgattgt aaccggtgcg
     1021 ccgctgggcc tggtggagtt taatgatgtc gcttactggc cgcagatcaa acaggtgctg
     1081 gagtggtcga aagatcacgt cacctcgacg ctgtttgtct gctgggcggt acaggccgcg
     1141 ctcaatatcc tctacggcat tcctaagcaa actcgcaccg acaaactctc tggcgtttac
     1201 gagcatcata ttctccatcc tcatgcgctt ctgacgcgtg gctttgatga ttcattcctg
     1261 gcaccacatt cgcgctatgc tgactttccg gcagcgttga ttcgtgatta caccgatctg
     1321 gaaattctgg cagagacgga agaaggggat gcatatctgt ttgccagcaa agataagcgc
     1381 attgcctttg tgacgggcca tcccgaatat gatgcgcaaa cgctggcgca ggaatatttc
     1441 cgcgatgtgg aagccggact aggcccggaa gtaccgtata actatttccc gcacaatgat
     1501 ccgcaaaata caccgcgagc gagctggcgt agtcacggta atttgctgtt taccaactgg
     1561 ctcaactatt acgtctatca gatcacgcca tacgatctac ggcacatgaa tccaacgctg
     1621 gattaatctt ctgtgatagt cgatcgttaa gcgattcagc accttacctc aggcaccttc
     1681 gggtgccttt tttatttccg aaacg
//
LOCUS       DAZFWP020000362         1687 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFWP020000362 DAZFWP020000000
VERSION     DAZFWP020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.365"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..187
                     /locus_tag="ACHK0Z_RS26240"
                     /old_locus_tag="pgaptmp_005248"
     CDS             <1..187
                     /locus_tag="ACHK0Z_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4505069.1"
                     /translation="QLRLGPADILESDENGIIPEQDRVITQVVILDADKKQIQCVVRP
                     LQILRADGTWENIGGMK"
     gene            505..654
                     /locus_tag="ACHK0Z_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /pseudo
     CDS             505..654
                     /locus_tag="ACHK0Z_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            875..1012
                     /locus_tag="ACHK0Z_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /pseudo
     CDS             875..1012
                     /locus_tag="ACHK0Z_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            1081..1662
                     /locus_tag="ACHK0Z_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             1081..1662
                     /locus_tag="ACHK0Z_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4505070.1"
                     /translation="MPLTSDINSSSFHLGMEVLRAQVAATGRGEFTMGGETVRIEYSP
                     TDGRFLASDGTGGLFTELLLLGFNNGPQALGERMLSILSGSDAGETQSQVTPQDKIYQ
                     CKFSVNTESLQCPSDATRCPIILETPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSV
                     HPLTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          482 a          291 c          459 g          455 t
ORIGIN      
        1 acaactgcga ctggggccgg cagacatcct ggagtcagat gagaatggca ttattccgga
       61 gcaggacagg gtaatcacac aggtggtgat actggatgca gataaaaagc agatacagtg
      121 tgtggtaaga ccgctgcaaa tcctgcgtgc tgacgggacg tgggaaaata ttggcgggat
      181 gaaatagccg acgggttcac aaaaaaccgg aggccggctc cggtttttgt tgtcatgtat
      241 gggggatgtt tgttattaga gtgtgaagta ataaacatgt taatacgatg gagtgaagga
      301 tgcctttaag gagcagggga gtgtccatct tgttccctgg agggggaatc ataaattctg
      361 attgcgaccg aggcatggaa cactactgta gttcagggaa gtagggcgat gaagctcatt
      421 cttgcgacgc gtaattatta tctggaatat ggtttgcgtt tgttactgaa aggatgccgt
      481 gtaattctgg ctcaagaatt ttttatgccg gaaaatcgca gggttattct ggatagtaaa
      541 gaatcctggt taataatctg tgatagtcag ttgggccatt taatgcgcag catgtttcag
      601 ggacgccgtt ttattcagct ggatctggaa gcgttgaaag ggggacatga tatacataat
      661 gctgtgcgta acagattgtg gacctggaac aaaaaagcac gggcactgac gatgtcagag
      721 atggtggtga tgtttggata tatctaccgt cagttgcgtc cgtcccatct cgccagtgag
      781 atgagggtaa acataaaaac agttaacact ttcctgtatt cagggctggc gaaaaatggg
      841 ctcaaaagaa gtagtgtaag gttgctggca atttctgaga acaagcgaat agggcattat
      901 tccggagcag gacagggtaa tcacgcaggt ggtgatactg ggtgcggata aaaagccgat
      961 acagtgcgtg gtgaggccgc tgcaaattcg gtgtgctgat gggacgaggg aaaaatttgt
     1021 ggatgacatt tgttgtggac ctttaagtct ggagttcaaa ttaaaacagg gagctttatt
     1081 atgccattaa cctcagatat taattcatct tcgttccatc ttggaatgga ggttcttcgt
     1141 gctcaagttg cagccactgg gcgtggagaa tttacaatgg gtggtgaaac tgtcagaatt
     1201 gaatatagcc caacagatgg gcgctttctg gccagcgatg gcactggggg attatttact
     1261 gaattattgc ttttagggtt caataatggg cctcaagctc ttggcgagag aatgttaagt
     1321 attctttcag gatcagatgc aggtgaaaca caatcgcaag tgactcctca ggataaaata
     1381 tatcaatgta agttttctgt taatacagag agcctccagt gtccctctga tgcaactcga
     1441 tgcccaatta tactggaaac accagaagaa ggggtatttg tcaaaaattc agatagttca
     1501 gcagtatgca ctttatttga tgttgatgca ctttctcgcg tggttaatga cggttcagtt
     1561 caccctctga cacgagctcc aataacccca tcaatgattg ttaaaccaga agagtgtaaa
     1621 tatgaccctg caaggggaag ctttattata aaagatagtt aagatgtttc aaatgaaaca
     1681 atattaa
//
LOCUS       DAZFWP020000363         1684 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFWP020000363 DAZFWP020000000
VERSION     DAZFWP020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.366"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(437..1414)
                     /locus_tag="ACHK0Z_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /pseudo
     CDS             complement(437..1414)
                     /locus_tag="ACHK0Z_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          414 a          392 c          383 g          495 t
ORIGIN      
        1 tgtgataaaa aaaatattta tctcgatgct acccaacata cgactcattt atttttctcg
       61 gtttctcctt gtatatatat gttgctttat aattttacta cgcacgtcag gcgtatctgt
      121 aacatagctc cgcattatgc aatgcgactt tgcatacaaa taggaataaa tctaatagct
      181 gtagatttgc tcctgtattt cctgaaccaa gcgagccagt acatgatgca tatgtctgga
      241 aaataattca gagtaaggat aagtccagct ttgttgaata aatcaggttt gggataagcc
      301 ccctcagttg gtagctactg caggtaatac gcacgtatta tgacattcgt gccctcgtca
      361 ttttgtttag tacacgcact gtagtggcac agaacttctg cgacctgacc atcctaaata
      421 gctgcgccga ataccgctac acttttaaca gcccatattt accaccgggg aatgggctca
      481 cggtttccat aaaataatga acctttttaa gcagttacca cattgaacgg cactgatgat
      541 tgcgcgttat cgtatcgtga agcgcctgct ataaccgctc aacatgattc acccacggcg
      601 agtaaactgg ctgataaatc actctgaact tcggattttc tttcagccag cgctgtgttt
      661 cccgactttt atggatgatg tagttgtcca ctatgagcgt gataggttta tcccgtcggt
      721 atgtcaattt cagatgtttc aacagactga taaataaggc tgagcttttg ctgttgccac
      781 ccacatagct gactttgccc gttccactat gtaaagcacc agccagatag tatttttcat
      841 tctgccccgg tgttaccaca tgtttttgct gtctgcgaag ttgccagtcc gcaccggttt
      901 tgggattaag atagatatac acttcatctt cataaaagac cggatgctcc gcactacatt
      961 cgtccagtgc tttgtgaatt gctgctttat gcgggttacg ggtacgcagg gttggcgcag
     1021 ccctgcgcca cacaagcccg actaatggca accagtggcg aacggttccg gcatgtaact
     1081 ggcactcggt tatctcgttg atttttattg ccagcagttc tgtactccag cgtgaacgct
     1141 gatagccaaa atatccggga gagtgtttta tcagttcacg aaacaatgtg cagatatgct
     1201 caaacggcca gcggcgggaa cgtcctgcgg gtaatgattt cagaccttca acactcgaca
     1261 gcgtgaacca gttaatccag cgcctgacgg aggaacgggc gcagcagagc gttctggcaa
     1321 cgtcactgac gcgagcgctc cggtgcagca tcagcatggc ggtcagcctg cgggcataat
     1381 ttttatcgtg agttttatag attgcttttt gcatcaggcg tcattcgtcg cggggaattg
     1441 gtgctatgat cggcatcgct cagtccggtt agggggggat ttttggcgat tgatcagatc
     1501 gcacaatccg ggctgagttc cctttcattg atctactatt cggcgcagct atttagttgg
     1561 tgattttgaa cttctgcttt gctactaagc agcctgcgtt ttcaggaaaa tacgtgatgt
     1621 gatcctgcaa ctcagcaaca gttcggttga ttcaacaaag cgttttttaa caataattct
     1681 tcat
//
LOCUS       DAZFWP020000364         1661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFWP020000364 DAZFWP020000000
VERSION     DAZFWP020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.367"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            29..1645
                     /gene="tir"
                     /locus_tag="ACHK0Z_RS26265"
                     /old_locus_tag="pgaptmp_005253"
     CDS             29..1645
                     /gene="tir"
                     /locus_tag="ACHK0Z_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGU4505071.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSDQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPTTTDPDTAASTAEAATKDRLT
                     QEAFQDPDKQKVNIDENGNAIPSGELIDDVVAQIAEQAKAAGEQARQEAIESNSQAQK
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARIPEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANASPTPGPARFV"
BASE COUNT          459 a          368 c          436 g          398 t
ORIGIN      
        1 tttacataaa ataaaaagga gacattttat gcctattggt aatcttggcc acaatcccaa
       61 tgtgagagct ttaattccac ctgcaccgcc attaccttca caaaccgacg gtgcaggagg
      121 tgcccgtaat cagctcatta actcaaatgg cccgatgggg tctcgtttgc tatttacgcc
      181 tataaggaat tctgttgctg atgctgctga ttctcgtgcc agtgatattc ccggacttcc
      241 tacaaatcca ctgcgctttg ctgcgtccga ggtatctttg catggtgcgc ttgaagttct
      301 tcatgataaa ggggggcttg atactcttaa ctctgctatt ggatcttcgt tattccgtgt
      361 tgaaactcgg gatgatggca gccatgttgc tatcgggcaa aaaaatggcc tcgagaccac
      421 tgttgtttta agtgatcaag agttttctag cttacagtcc cttgatcctg aaggtaaaaa
      481 caaatttgta tttactggag gccgcggtgg cgcagggcat gctatggtca cggttgcttc
      541 agatatcgcc gaagcccgtc agaggataat agataaatta gaaccaaagg atacaaagga
      601 gacgaaggag ccaggggatc caaatagtgg cgagggaaaa atcattgaaa ttcatacctc
      661 aacctcaact tctagcctcc gtgcagatcc taaactttgg ttgtcattgg ggactattgc
      721 tgcaggtctg atagggatgg ctgcgacggg tattgcacag gctgttgcgt tgactccaga
      781 gccggatgat ccaactacta ccgatcctga tactgcagca agcacagctg aagctgcgac
      841 aaaagatcgg ttaacgcagg aggcattcca ggatccagat aaacaaaaag ttaatatcga
      901 tgagaacgga aatgcaattc cgtcagggga attaatagat gatgttgttg cgcaaatagc
      961 agaacaagct aaagcggcgg gggaacaggc cagacaggaa gctattgaaa gtaattctca
     1021 ggcgcagaaa aaatatgatg agcagcatgc taaacgcgaa caggaaatgt ctctttcatc
     1081 gggggttggc tacggtatta gtggggcgct gattcttggc gggggaattg gtgccggtgt
     1141 tactgctgct cttcatcgga aaaaccaacc ggcagaacaa acaatcacta cacgtacagt
     1201 agtcgataat cagcctacga ataacgcatc tgcacagggc aatactgaca caagtgggcc
     1261 agaagagtcc ccggcgagca ggcgcaattc gaatgccagc cttgcatcga acgggtctga
     1321 tacctccagt acgggcacgg tagagaatcc gtatgctgac gttggaatgc ccagaaatga
     1381 ttcactggct cgtattccag aggaacctat ttatgatgag gtcgctgcag atcctaatta
     1441 tagcgtcatt caacattttt cagggaacag cccagttacc ggaaggttag tgggaacccc
     1501 agggcaaggc atccaaagta cttatgcgct tctggcaagc agcggcggat tgcgtttagg
     1561 tatgggagga ttaacggggg ggggcgagag cgcagtaagt actgccaatg cctcaccaac
     1621 gccgggaccc gcacgtttcg tttaaatata tccgtgagta t
//
LOCUS       DAZFWP020000365         1652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFWP020000365 DAZFWP020000000
VERSION     DAZFWP020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.368"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(175..798)
                     /locus_tag="ACHK0Z_RS26270"
                     /old_locus_tag="pgaptmp_005254"
     CDS             complement(175..798)
                     /locus_tag="ACHK0Z_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HGU4505072.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDRLSSKLVAISEELGKMGCSFYSYDVSSTSIYLTVSGRRVGWHSYG
                     KDGNGKDILLPTPIKDKCMFDAEHEITKRFDEICALQQKLEAKKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEAA"
     gene            complement(860..928)
                     /locus_tag="ACHK0Z_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /pseudo
     CDS             complement(860..928)
                     /locus_tag="ACHK0Z_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT          401 a          374 c          337 g          540 t
ORIGIN      
        1 atatcctcat agataaaaaa tcgccctcac actggagggc aaagaagatt tccaataatc
       61 agaacaagtc ggctcctgtt tagttacgag cgacattgct ccgtgtattc actcgttgga
      121 atgaatacac agtgcagtat ttattctgtt gtttatgcca aaaataaagg ccgactatgc
      181 ggcctcggaa ggaagtccaa tcatcttatt caaatcttct acccgtaaag caggaagtgc
      241 tgtacttgct ttatctgctt cttttggtag caattctttg ctttcaggcc aaacttcaat
      301 aagtcgctta actgttgtga ctgagttcaa agcagcccat acatttgatt cgatatcctt
      361 tttcttggct tcaagttttt gttgcaatgc gcagatttca tcaaaccttt ttgttatttc
      421 gtgttctgcg tcaaacatgc atttatcttt gatcggagta gggagcaata tatctttgcc
      481 gttgccgtct ttcccatatg aatgccatcc aacccttctg ccagatacag tcagataaat
      541 tgaagtagaa ctaacatcgt atgagtaaaa tgaacatccc atctttccaa gttcttcact
      601 tatagctacc aacttggatg ataaccgatc cacttcctca gttttctttt taccgccaaa
      661 cgcaataact ctggcgtcaa gtgcaagctg gttctttaac tttgttactt cttcaagttc
      721 agtgaacacc ccggacttaa ttaaagcgtt acgagcgatt tcctctttca ttctcgtagt
      781 taagcggatt gatgacatat taattcctct caaataagtg gtttgctgcc taatttcatt
      841 ttctggcgac caacacaagt catcttgccg tcagttgttt tgatttccgg tagcctgccg
      901 cgtaaagagc tacgtttgga agacatacac cagtttctgg ttgcttatgt ccaaactcat
      961 tcgcgtacac aatggccgct cgctccagat tgcgtctgta ttctttctgt tgccagatca
     1021 cgtcctgtgc catgaactta attggcttag cgtcttctat gcgctcaggc gtttcgtgag
     1081 tacctttagc ctgaatctgc gctctgctta gagtagggcg gtgtaatact tctgaactta
     1141 ttgcttcttc gcgggccagc acgccgttag ctaatgcctt tgcctttaaa cgctcacgac
     1201 gacgagaacg tgaattgcct ttgaactgag ttctgcgtgt catatagacc tcctgatgaa
     1261 ctttggtggt gtggtaggtg ggagacccat ttcgacctgt ttcggcctac ttcaattcgg
     1321 caatagtccc gcaggcctcg ccgctttacg tgcgacatat tcccgtccat gaacccttca
     1381 ccacacccca aagttcactt tggttattgc gctttgtcag cgccgtagat tcatattcga
     1441 atcgttgtat attcaccgcc ctggtgagta gtgcgtcctg ctgatgggtg aactttatcg
     1501 gaatgataaa ttaatggcaa tagcaaaatg ataaattctc ttggttttca aatatcgtat
     1561 tgattcttat ggtgttttat tttgttgtag gaattcgaca ggataaataa agagatttga
     1621 gggagatctg gattgcgttg tttagcaagt tg
//
LOCUS       DAZFWP020000366         1632 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFWP020000366 DAZFWP020000000
VERSION     DAZFWP020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.369"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            188..757
                     /locus_tag="ACHK0Z_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     CDS             188..757
                     /locus_tag="ACHK0Z_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGU4505073.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1017..1418
                     /locus_tag="ACHK0Z_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             1017..1418
                     /locus_tag="ACHK0Z_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4505074.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVLDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            1406..>1632
                     /locus_tag="ACHK0Z_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             1406..>1632
                     /locus_tag="ACHK0Z_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4505075.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAE"
BASE COUNT          393 a          442 c          438 g          359 t
ORIGIN      
        1 acagccttac ccgtaaccgg ttttttacat taaaaaaaca acgctgaaaa ttcaaccgtc
       61 agtccggaga cgaccgttcg ggttatcaca gagtgcctga gacagtgtcc tgccggaggt
      121 cacttcaggg actctgcgca ttttttacga cgtggctatt ccgtatgaac catacggaga
      181 tttaaccatg acctacaaat acaacccctt ctggcagcaa cgtattcgtg agacggtgcg
      241 gcacgcactg aatgttcatc cccgcctgac ggcattgcgg gttgacctgc gtttcccgga
      301 tgtaccggca gcaacggacg cagctgtgat atcccgcttc atcaatgccc tgaaagcccg
      361 aatcgacgct taccagaaac gtaagcatcg ggaaggtaaa cgcgtgcatc ccacaaccct
      421 gcattacgtc tgggcccggg agtttgggga gtgcaaaggt aaaaaacact atcacctgat
      481 gctgctggtt aaccgggata cctggtgtcg tgccggtgat taccgtgctc cgggatcact
      541 ggccgggatg attaaacagg cgtggtgcag tgccctggga gtggatgtcg ggtgccatgc
      601 cacgctggtg cattttccgg cctggccggc ggtgtggctg gaacgcgatg atgacactgg
      661 ctttcagcag gtgctggaac gtgctgacta tctggcgaag gaacatacca aagctcactg
      721 caccggtgag cgcaattttg gctgtagccg gagttgagca cgactggcgc gccaataccc
      781 ggcaaaccaa aagtatgggt tgttgtctgg cggcaacgat gtatccactc gcccactgac
      841 tgttttttac gtcagtgttc cattgtgtga caaccacagt gctgtaacag caaccagcct
      901 gctttaccca aactccagtc cagattttaa ctgaaccgcc attcacaccc tgaccacgct
      961 gcccgtaccg ggacggctgt tgcctgcgtg tcgtctggcg gctgaggaat atgactatgt
     1021 acgcaaaatc ctttatcgct cttgatggca acggacgtct gacgggcgcc cgtactgcac
     1081 aggccgcacc ttatgctaac tacacctgcc acttgtgtgg cagtgcactc agataccatc
     1141 cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg acagagcacg
     1201 gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt aaacgtctgc
     1261 agcaattcgt actggatgcc ttacccgtgg tgcgtaaagc cagctggcac tgcagacaat
     1321 gtcaccacga ttattatggg gagcggtact gcacacactg ccagaccgga ggattcagta
     1381 ttccccggac aactcaggag gaaatatgcg aattctgaac tgttatatgg cgaatgacag
     1441 caaaggccat tttgttacgg cgaaagaagc agcgaagcac aaccgccagg acgttttgtg
     1501 ctgtgtgtcc tgtggatgcc cgttaacact tcagcggggc aatgacggac aaccaccgtg
     1561 gtttgaacat gaccagatga ctgtcgctga gaaaatcctg ctgcgatgca cctggcttga
     1621 cccggcagag aa
//
LOCUS       DAZFWP020000367         1614 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFWP020000367 DAZFWP020000000
VERSION     DAZFWP020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.370"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..413
                     /locus_tag="ACHK0Z_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     CDS             6..413
                     /locus_tag="ACHK0Z_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HGU4505076.1"
                     /translation="MNNNVKLIHFDKKGDNRGFLVVVEQNKDIPIEIKRVYYMFDTGS
                     DVRRGYHAHKKLTQIAIPVKGSCKFLLDDGKSVEHVTLDSPSVGLIIEPMIWHEMYDY
                     SSDCVLMVLADDFYDESDYIRNYADFIREITNA"
     gene            406..939
                     /locus_tag="ACHK0Z_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             406..939
                     /locus_tag="ACHK0Z_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4505077.1"
                     /translation="MRNLSLFTKNTKLIPVEINDAEFIYSLRIDSRLNKHISTINGTV
                     IEQAEWIEDYKKREENNEEFYFIIKRTDNDEKIGTIRLYNITKDDGFCWGSWILNDDK
                     MKSSAIESAYLIYKFGFLHKNYKYSYFQVDKKNKEVIAFHKKTGATIIKEDDVNFHFE
                     FYVENFIRLREKYSKYF"
     gene            951..>1614
                     /locus_tag="ACHK0Z_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             951..>1614
                     /locus_tag="ACHK0Z_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGU4505078.1"
                     /translation="MIDFLNLKRINAQYEVELKDACSRVIDSGWYIMGTELKTFEEKF
                     ANWCGVKHVIGVANGLDALILVLRAWKEMGKLNDGDEIIVPANTYIASVLAITENNLV
                     PVLTEPDPLTFNLSAGGIRKVITGKTKAILPVHLYGQISPMAEIMEIAAEFGLLVLED
                     CAQAHGSSIDGRKAGSWGHAAGFSFYPGKNLGALGDAGAITTSDDQLADILTAIRNYG
                     SHE"
BASE COUNT          565 a          207 c          341 g          501 t
ORIGIN      
        1 atcgcatgaa taataacgta aaacttatac actttgataa gaaaggtgac aaccgaggct
       61 ttcttgttgt tgttgagcaa aataaagata tcccaattga aattaaacga gtttactaca
      121 tgtttgatac tggttctgat gtaagacggg gatatcacgc acataaaaaa cttacacaaa
      181 tagcaatccc tgttaaggga tcttgcaagt ttttgcttga tgacggtaaa tcggtggaac
      241 atgttacatt agattcaccg tcagtgggat taattataga accaatgatt tggcatgaaa
      301 tgtacgatta ttcatcagat tgtgttttaa tggtgctggc agatgacttt tatgatgaaa
      361 gtgattacat tcgtaattat gctgatttca tcagggagat aactaatgcg taatttgtcg
      421 ctctttacaa aaaatacaaa attaattcca gttgaaataa atgatgcgga atttatttat
      481 tcactgcgga tagattctcg tctcaataag catatttcta caataaatgg gacggtaatt
      541 gaacaggccg aatggattga agattataaa aaaagagaag aaaataatga agagttttat
      601 tttataataa aaagaactga taatgatgaa aaaattggca cgataagact ttataacatc
      661 acgaaagatg atggtttttg ttgggggagt tggattctaa atgatgacaa aatgaaaagc
      721 tctgcaatag aaagtgccta cctgatatac aaatttggtt ttttacataa aaactataag
      781 tattcatatt ttcaagttga taaaaaaaat aaagaagtaa tcgcatttca taaaaaaaca
      841 ggagccacca taataaaaga ggatgacgtg aattttcact ttgaatttta tgtggaaaat
      901 tttattcgtt taagagaaaa atatagtaaa tatttttgag gggaaagaaa atgattgatt
      961 ttttgaattt aaaaagaata aatgctcaat atgaagtgga attaaaagat gcttgctcca
     1021 gagttattga ttctggttgg tatattatgg ggacggagtt aaagacattt gaggaaaaat
     1081 ttgcaaattg gtgtggagtt aagcatgtta ttggtgttgc caatggctta gatgcactaa
     1141 tccttgttct tcgggcttgg aaagaaatgg ggaaattaaa tgacggagat gaaattattg
     1201 tacctgccaa tacatatatt gcatctgttt tggcaataac tgaaaataat ctagtccctg
     1261 tattgactga accagatcct ttgacattca acttaagtgc gggtgggatt cgtaaagtaa
     1321 ttacagggaa aacaaaagca attttgccag tgcatttgta tggacaaata tctccaatgg
     1381 cagaaataat ggagattgcg gcagagtttg gtttattggt tttagaggat tgcgctcaag
     1441 ctcatggttc ctctatcgat ggacgcaaag ctggcagttg gggtcatgca gctggattca
     1501 gtttctatcc aggaaaaaat cttggcgctt taggtgacgc aggtgcgata acaacgagtg
     1561 atgatcaact tgcagatatt ttgacagcta ttagaaatta tggttctcat gaaa
//
LOCUS       DAZFWP020000368         1614 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFWP020000368 DAZFWP020000000
VERSION     DAZFWP020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.371"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            33..908
                     /locus_tag="ACHK0Z_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             33..908
                     /locus_tag="ACHK0Z_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4505079.1"
                     /translation="MFSIRSQLPISASVSIPAEQSQSISTIPGGRKIETVPEKEGLLV
                     FLGTKSVGEYALNILGQNVSRVTTGKKPYDILFLHEATKQDFDKKKTEFTFPGANRSY
                     LQSSNTDVAAAAAISIAATEMKTILPKDLTPEKYNKIYLPGDGSAGLPLLKCGDEFLS
                     PTDIVNRLVEHNLHEVEDIRLTSCHSANITKNNDFSPEEIEKSTKTNSGWLASALFGQ
                     KRSLAEHVYAEFEGRGINVSVSGYHGTGVFYVPEHGKPTTHLRSTTVPATPEQTVRRS
                     DYRVSLGRTQPTDID"
     gene            complement(1148..1321)
                     /locus_tag="ACHK0Z_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /pseudo
     CDS             complement(1148..1321)
                     /locus_tag="ACHK0Z_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          513 a          371 c          298 g          432 t
ORIGIN      
        1 ctcacattta aacaaactaa gaaggaggtt gtatgtttag tataagatcc cagctaccta
       61 taagcgccag cgtatcaatt ccggcagaac aatctcaatc catctccaca attcctggag
      121 ggagaaaaat agagacagtg ccagagaaag aaggattgct tgtgttttta ggaacaaaat
      181 ccgtaggtga atatgctctt aatattcttg gtcaaaatgt ttcaagagtc acaacaggga
      241 aaaaacctta tgatatatta ttcctgcacg aagctacaaa acaagatttt gataaaaaga
      301 aaacagagtt tacctttcct ggtgcaaaca gaagttatct acaatcaagt aacaccgatg
      361 ttgctgccgc agcagctata agtattgcgg caacagagat gaaaactatc ctaccaaagg
      421 atttaacacc agaaaaatac aataaaattt atctgccagg ggatggttct gctggcctac
      481 ctcttcttaa gtgtggagat gaatttttat caccaactga tatcgtcaat cgtctggtag
      541 aacataatct tcatgaagta gaagacatca gattaacatc ctgtcactca gccaacataa
      601 caaaaaacaa tgatttctct cctgaagaaa tagaaaaatc aacaaaaacc aacagcggct
      661 ggttggctag tgcattattt ggtcaaaagc gttcattagc agaacacgtc tatgccgagt
      721 ttgaaggccg tggaattaac gtttctgtat ccggttatca tggcactggt gttttttatg
      781 ttccagagca tggcaaacca acaacgcatc tacgctccac aacagtgcct gcaacacctg
      841 aacagacggt aagaagaagt gactacagag tctcccttgg cagaactcaa cccacagata
      901 ttgactaatt gagtctgcat ataggtttgt gtcattacct gatgcgctac gcttatcaga
      961 cctatattag atcctgcaat atgtttaatt tgtaggcaga atagagcgtt cacaccacat
     1021 caggtataaa caaagtacag cttgccaaaa acctgaataa ccccgaattt acggggttat
     1081 tccgcttaaa catgcccata acatcacttc tgatttgtat tgcttacgac ggatacaatt
     1141 tctctgctca gctacgaata tcatcatact cgcgcgtttt atcgaactcg tgtttcgcaa
     1201 acggacatag cgggataatc ttccgcttct cccggcgcat tttttccacc accttcgcca
     1261 ccagccgttt accaatcccc tgccctttca ggctttcatc aacatcggta tgttcgataa
     1321 tggtcagtta tcagggataa ccctgtgagt acaacgcaat ctgttgctac tgggtagact
     1381 ggtctgctga tcacactcac agatttattt cacatatatg aactcatgta tttgatctct
     1441 ctcttatata tttaatacca atcagacagt cttatcccac cagtgatacc tgtccctgcc
     1501 cacaaggcac gggctgcccc tgtagtggca cactaaattt ggccacctga tgaaaggtga
     1561 tattctcacc acaacacaaa acaggtgact taatgaacaa gaaaaccaaa cgaa
//
LOCUS       DAZFWP020000369         1594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFWP020000369 DAZFWP020000000
VERSION     DAZFWP020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.372"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..615
                     /locus_tag="ACHK0Z_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             <1..615
                     /locus_tag="ACHK0Z_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4505080.1"
                     /translation="AKIPVPADGKSITPDDVRPMIEQMVKEAVSHIPVPRDGRDYDPD
                     VLQKAVLEAVSALPAPQDGRDAMALEILPAIDDQKSFPRGSYATHQGGLWRAYEKTYG
                     MRGWECLVDGVADIDVSMTGERSFSVVVRQSSGQRTEKTFSLPVMLYRGVFRTGETYH
                     PGDTVTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
     gene            612..938
                     /locus_tag="ACHK0Z_RS26325"
                     /old_locus_tag="pgaptmp_005265"
     CDS             612..938
                     /locus_tag="ACHK0Z_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4505081.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGGLPFSVSVLIYDL
                     RCPTVL"
     gene            949..1299
                     /locus_tag="ACHK0Z_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             949..1299
                     /locus_tag="ACHK0Z_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4505082.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            1296..>1594
                     /locus_tag="ACHK0Z_RS26335"
                     /old_locus_tag="pgaptmp_005267"
     CDS             1296..>1594
                     /locus_tag="ACHK0Z_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HGU4505083.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANELKDE
                     VVSRAPVRRGKLRRNVVVLSWRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNA"
BASE COUNT          342 a          373 c          552 g          327 t
ORIGIN      
        1 gcgaaaatac cggtaccggc agacggtaaa agtatcaccc ccgatgatgt gcgtccgatg
       61 attgagcaga tggtgaaaga ggcagtgagc catattcctg ttccgcgtga tggtcgtgac
      121 tacgatccgg atgttctgca gaaggcggtt ctggaggcgg tgagtgccct gccggctccg
      181 caggacgggc gtgatgccat ggcactggaa atactccccg ccattgacga tcaaaaatcc
      241 tttccccggg gctcgtatgc cacacaccag ggtggactct ggcgggcgta tgaaaaaacg
      301 tacgggatgc ggggatggga atgcctggtt gacggggtgg cggatattga cgtcagcatg
      361 acgggtgaac ggtcgttctc tgtggtggtc cggcagagca gtggccagcg tacggaaaaa
      421 acattttccc tgccggtgat gctctaccgt ggtgtgttca gaaccggcga aacttaccac
      481 cccggcgata cggtgacgtg ggggggctcg ttgtggcact gcaacagtat gaccggtgat
      541 aagcccggag aagctcattc atcaggctgg actctggctg caaaacgtgg gcgggatgca
      601 ggaggtggaa agtgacagca ttactgacac tggaagaaat caaggcacat ctgcgtgttg
      661 accatgacgc ggatgatgag atgctgatgg acaaggttcg tcaggctacc gccgtgctgc
      721 tggcctacat ccagggcagc cgggataaag tgattagtga ggacggtgag ctgatccctg
      781 gtgaggcatt aacccggatg aagggggctg ccatgcgact gaccgggatg ctgtaccgga
      841 atccggatct tgctgagcgg gaagacctcg tgcagggggg actgccgttt tctgtttccg
      901 tgctgattta cgatttgcgt tgtccgacgg tgttatgagg agggggggat ggcaatatct
      961 gcaggtcgtc tgacacagat gatcagtgtt ctgaacccgg tgttaacccg taatgctgcc
     1021 ggagaaatga cggaagaatg ggtgtcatgc gggaaaattc atgcggatat ccggggcagg
     1081 agcagccggg agcggatgca gtccggtgcg gaaatggcgc aggcggaaat ccgcatctgg
     1141 gtgcgcggtc agtctggtcg tgaaatcacg gcggcgtcac gacttcatgt gctgagtggt
     1201 ccctggcgtg accatgtcct gaatgtcgtc ggggtcccgg ttccggatgc aaccggcggg
     1261 cgtctggaaa ttctctgtcg gctgggaggg gaaaaatgat cgaaaccctg ctggattttt
     1321 cggggctgga ggacatcagc cgcgatttgc agcttctgag tggtgcggaa aacaaccggg
     1381 tgttgcgtga ggcaacccgc gcgggggcga atgaactgaa agacgaagtg gtgtcacggg
     1441 caccggtgcg caggggaaaa ctgcgccgca atgtggtggt cctttcctgg cgttcccgcg
     1501 atggcgggat ggaatccggt gtgcatatcc gtggtgttaa tccggacacc ggcaacagtg
     1561 ataacaccat gaaggcggat aatccccgca atgc
//
LOCUS       DAZFWP020000370         1594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFWP020000370 DAZFWP020000000
VERSION     DAZFWP020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.373"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1594)
                     /locus_tag="ACHK0Z_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             complement(<1..>1594)
                     /locus_tag="ACHK0Z_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4505084.1"
                     /translation="IEICDDDYAGISIGGRVLAVNSQTRTLTLDREITLPSSGTTLIS
                     LVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIREND
                     DGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVL
                     ARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAAN
                     AWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIT
                     DIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAE
                     GYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIEQT
                     KDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDV
                     FLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTINENCQIKG
                     KLSANQIEGDIVKTVSKSFPRTS"
BASE COUNT          307 a          493 c          412 g          382 t
ORIGIN      
        1 tgctcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcaccttca atctggttgg
       61 ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg agcgtccctg
      121 agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt ccgtctgatg
      181 tcagggaaaa taccggaggg ctgccaccgc tggtaatggt gggggctgtc aggcgcttca
      241 ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc
      301 cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagcttgc
      361 cttcctccgt gtcctccatg ctgagaccaa tacccgcgac ataatgtttg ccgtctttgg
      421 tctgctcaat tttgacgccc cacatggcat tccatttatc gttagcgtcc ttccactctt
      481 tcgaaaactc atccagtctg ctggcgttat cctccgtcag atcaactttt tccagcagct
      541 ctttaccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa ccttccgcat
      601 catcgctcgc ccgaccgacg gcctccacga atgccgattt gccaacggtg ttcacactgc
      661 ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct atccagtaca
      721 gccccgtgcc aaggtagcgg gctgtggttt caacctgcct gatatcggta atccgctttt
      781 ccgagaacca gaactcaaac tgtaccgtcg gatcataaac ggcaagatgc ggcgttgcgg
      841 ttatctgaaa atagcccggc gtcagctcaa tccgcgacgg tgctgccggt gcggcaatcc
      901 ggaacgatac cgacgccgga tcgccctgct gcccccaggc atttgccgcc cggactgtca
      961 gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt ggtttccgtc gtccgggccg
     1021 tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc ataaagctca
     1081 cgcccttcac caccttcggc gtgtcccagc gcgccagcac ctgatattcc ccgctgtctg
     1141 cggtgacttc ggcagtcagg tgctgcaccg cgggcggcgt gacgccattc accgtgccgc
     1201 tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ttctttttcg ggtacatgct
     1261 gcacggcggt gatggcatac gtgccgtcgt cgttctcacg gatactcacg cagcggaaca
     1321 ggcgctggcg caacgtcggc aacttcagcc cccatacgct gtattcagca acgccgtcag
     1381 gaacacggct cactttcacc ttcacgccgt cggtgacgga ctggacctcc acgctgaccg
     1441 gattaccctg cccgtcaacc aggcttatca gcgtggtgcc ggaggatggc agcgtgattt
     1501 cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc cagcacgcga ccaccgatgc
     1561 tgataccggc atagtcatca tcgcagattt caat
//
LOCUS       DAZFWP020000371         1585 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFWP020000371 DAZFWP020000000
VERSION     DAZFWP020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.374"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(66..278)
                     /locus_tag="ACHK0Z_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /pseudo
     CDS             complement(66..278)
                     /locus_tag="ACHK0Z_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            428..1096
                     /locus_tag="ACHK0Z_RS26350"
                     /old_locus_tag="pgaptmp_005270"
     CDS             428..1096
                     /locus_tag="ACHK0Z_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4505085.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1462..1575
                     /locus_tag="ACHK0Z_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             1462..1575
                     /locus_tag="ACHK0Z_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HGU4505086.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT          491 a          350 c          273 g          471 t
ORIGIN      
        1 aaactgatat tactttaatc aaaagttgac gtgccagatt cgaagtaaat ttataaaata
       61 tcaaatcaat atacattttg caccttcgag atagtaatga ctgttactgt aacatgagtt
      121 acaaacagct tcatacccag agctactgat gtatcaactc gattcaacaa tacccgatat
      181 ttattccatg cctccagcaa cgatctttct tcctccgttg cgatttccag atctacagca
      241 tcctgcagtg gcgcaatata ctcactgaat tcctggatgt agaactgtgt ggtgacggtc
      301 ttccagccat tcagctcctg ctgtatcgaa gcataccagg ctatttcaat atcgctatgc
      361 tgcggcagca tttaacccct tgtaattcat cgccataatt gatttaattc acaaataaaa
      421 ctataacatg gtgaaattaa tgaaaaaaaa cacagatgat ggggctaaaa tttacacacc
      481 acttacccta aagctttatg actggtgggt tttgggagta tcaaatcggc ttgcatgggg
      541 atgtcctaca aaggaacacc ttcttccaca ctttctggaa catttaggta acaaccatct
      601 ggatattggt gttggaactg ggttttacct tactcacgta cctgagagta gtctgatatc
      661 tttaatggat ttgaacgaag ctagcctgaa cgcggcatct acaagggctg gggaatcaaa
      721 aattaaacat aaaattaacc atgatgtttt tgaaccttat cccgcggcgt tacatggtca
      781 atttgattcc atttccatgt tttaccttct tcactgcctg cctggaaata tatctacaaa
      841 aagctgtgta atacgcaatg cggcgcaggc cttaactgac gatggaactc tatacggagc
      901 cacaattctt ggcgatggag ttgtgcacaa tagcttcggt caaaaactga tgcgcattta
      961 caatcagaaa ggcatctttt caaacacaaa agattccgaa gaaggcttaa cacatatact
     1021 ctcagagcat ttcgagaatg ttaaaaccaa ggttcaaggt actgtagtaa tgttttccgc
     1081 ttcagggaaa aaatagcatc caaccgcagc acgttcttgc ataaggcgtg tctgcggttt
     1141 ttcaactatt cagatacatc actcccatca cattcattcc tccgcatcat aggcatatag
     1201 gctatatcac cttgatattt ttcttcttca gataaaaact gttatctatg tatactttta
     1261 aacccaatcc gtgtagagtc tctgcataag atagtttgca gttgccactt cagcttgcgc
     1321 cataaaccgc ctgatttttg ccgccacctg ttagcattcc tgtatacctg aaacgacaat
     1381 gtttatctac gaactttaag aacacccagg ataaaaattg tcaactatat catatataac
     1441 acattactaa ttcgaggcta tatgaacagc atactgataa tcacttcgct ccttatcata
     1501 ttcagcattt ttagtcatgc tctaataaaa ttagggattg gcatatccaa taacccagac
     1561 aaaaccgatg tataagtcaa catat
//
LOCUS       DAZFWP020000372         1578 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFWP020000372 DAZFWP020000000
VERSION     DAZFWP020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.375"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..123)
                     /locus_tag="ACHK0Z_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /pseudo
     CDS             complement(1..123)
                     /locus_tag="ACHK0Z_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspG"
     gene            183..880
                     /locus_tag="ACHK0Z_RS26365"
                     /old_locus_tag="pgaptmp_005273"
     CDS             join(183..432,432..880)
                     /locus_tag="ACHK0Z_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HGU4505087.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(904..1122)
                     /locus_tag="ACHK0Z_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /pseudo
     CDS             complement(904..1122)
                     /locus_tag="ACHK0Z_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
     gene            complement(1298..>1578)
                     /locus_tag="ACHK0Z_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             complement(1298..>1578)
                     /locus_tag="ACHK0Z_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4505088.1"
                     /translation="GERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISK
                     RYTQRIERHNLNLRQHLARPGRKSLSFSKSVELHDKDIGHYLNIKHYQ"
BASE COUNT          417 a          384 c          377 g          400 t
ORIGIN      
        1 aggaatatca gctgtatcaa tatcactaag gggaatccct ggcacgcgta gcattcggag
       61 atacacatca ccgccatact gtataacaac tgatgcgtca tcgccataaa aagtattaaa
      121 aagggcaggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
      181 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
      241 acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc
      301 aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg
      361 ccatgaatgg cgttggatgc cgggcaactg cccgcattat gggcgttggc ctcaacacga
      421 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
      481 tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg
      541 ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt
      601 cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
      661 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
      721 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
      781 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa
      841 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttaccaaaag
      901 ggcagattct tgcctagcca tttccagaac ttcattgtgc tggttaccaa caagatcata
      961 ctttttatat aaagcagacg aatcattcat atcttcaaag atttctgctg ttgaaccttg
     1021 ccctattaac ttaccgagaa taggctccgt tactacgcca gtatgagaat tattattctc
     1081 aaatgggaaa ggtgtttgaa taacagtgga tattattttc atgacacgcc cttgttatga
     1141 aatttaaaga ttacaagata tttttacata aaataatatt acgcgctacc acctcagaaa
     1201 ccacgtaagt acatatgcta ttacatagat aaccgccatg atcagggaag ccccatacaa
     1261 gcacagtgta gagaggcctt taggtaatga ctccaactta ctgatagtgt tttatgttca
     1321 gataatgccc gatgtccttg tcatgcagct ccaccgattt tgagaacgac agtgacttcc
     1381 gtcccggcct tgccaggtgt tgtctcagat tcagattatg tcgctcaatg cgctgagtgt
     1441 aacgcttgct gataacgtgc agctttccct tcaggcgtga ttcatacagc ggccagccat
     1501 ccgtcatcca taccacgacc tcaaaggccg acagcaggct cagaagacgc tccagtgtgg
     1561 ccagagtgcg ttcaccga
//
LOCUS       DAZFWP020000373         1576 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFWP020000373 DAZFWP020000000
VERSION     DAZFWP020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.376"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            202..>1576
                     /gene="ygeH"
                     /locus_tag="ACHK0Z_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             202..>1576
                     /gene="ygeH"
                     /locus_tag="ACHK0Z_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HGU4505089.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTLESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          571 a          270 c          268 g          467 t
ORIGIN      
        1 ttagagaaca atctacttta tcaaaattga ataaggctat ttcagattac atcactgaat
       61 tcctaagata aattagtgaa cacaaccata gaatgtcgaa tgtaatttcc tgtagcgtta
      121 atgacacata aatatgaata gccataattt ctattgctta agcaggatgc aagaaaccaa
      181 ttttttcata gaggttaact aatggactta gaaaataaat tctcatatca ttttcttgag
      241 ggattaacgc tcacggaaga tggaattctt actcaaggaa atgagcaagt ttatattcca
      301 cagaaagagt taggtgtatt aatagtatta cttgaatccg ctggtcatgt tgtactgaaa
      361 gatatgatca tcgaatcagt atggaaaaat attattgtta gtgacgagtc cctgacaaga
      421 tgtatctatt ctttgcgctg catttttgaa aaaattggct atgatcgttg catagaaaca
      481 atctaccgga aaggttatcg tttcagcggg caggttttca aaactaaaat taatgaagat
      541 aatacttcag actattccat agctatattc cctttcacta cttcgttgaa tacactggat
      601 ccattaatac ttaatcagga attagtgcaa atcatttcaa ataaaaaaat cgatggtctc
      661 tatacctatc cgatggctgc gacaaatttt tgtaatgatc acatatctca aaattcattc
      721 ttgagcagat tcaagccaga ttatttcgtt acaggaagaa taaaccagaa taatgcagtg
      781 aacactttat acattgagtt gatcgacgct aaaaaccttt tcctcatcgc cagtaatcat
      841 ctccctgttg atgaactaca taatacatca caatttatta tagataatat ccttcaaacg
      901 gtacataaac cagaacgatc tgtaagatta gctaagcagg accaaggata taagaatcat
      961 tatttatcag atgaaatgtt agccggaaag aaagaacttt acgacttcac ccttgaaagc
     1021 atttacaggg ccatgactat atttgataga ttacaaaata aaagtgatat acagacgcta
     1081 aaaacagaat gttattgcct tctagcggaa tgccatatgt ctttggcact tcatggaaaa
     1141 agtgaacttg aacttgctgc tcaaaaagca ttagagcttt tagattatgt atcagacata
     1201 accactgtcg atggaaaaat tttagctatt atgggactga taactggtct gtctggacaa
     1261 gcaaaagtat ctcatatctt atttgaacag gctaagatac actcaactga tatagcctct
     1321 ctctactact atagggcact agtccacttt cataatgaaa aaattgaaga ggcaaggatt
     1381 tgtatagaca aatcactaca actcgaaccc agaagacgaa aagcagttgt gataaaagaa
     1441 tgtgtagata tgtatgtgcc taacccgctc aaaaacaaca ttaaactcta ctataaagaa
     1501 actgagagtg aaagccatcg agttataatt gacaacattt tgaaattaaa gcagctgacg
     1561 agaatttgta tgcgat
//
LOCUS       DAZFWP020000374         1563 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFWP020000374 DAZFWP020000000
VERSION     DAZFWP020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.377"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..499
                     /locus_tag="ACHK0Z_RS26385"
                     /old_locus_tag="pgaptmp_005277"
     CDS             <1..499
                     /locus_tag="ACHK0Z_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4505090.1"
                     /translation="RLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTKEA
                     NVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEGTA
                     ENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALINV
                     IYTWQ"
     gene            558..1472
                     /locus_tag="ACHK0Z_RS26390"
                     /old_locus_tag="pgaptmp_005278"
     CDS             558..1472
                     /locus_tag="ACHK0Z_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4505091.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
BASE COUNT          450 a          329 c          355 g          429 t
ORIGIN      
        1 aagacttcac aagaggttcc tgttagctac gttttgcgcg ttattcacag caactctcca
       61 ggccgccgat gtcactatca ctgttaatgg tcgggtagtc gctaaaccct gcactattca
      121 aaccaaagaa gctaacgtta atctcgggga tctttatacg cgcaatctgc aacaacctgg
      181 ttctgcatct ggctggcaca atattacttt gtcattaacc gattgtccgg ttgaaacaag
      241 tgcagtgacg gcaatcgtga caggttcaac tgacaatacg ggttattaca aaaatgaagg
      301 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
      361 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      421 aagagctatc acggtgaatg gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt
      481 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      541 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      601 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      661 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      721 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      781 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      841 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      901 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      961 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgctcgt attcatatgt
     1021 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
     1081 ataacaatgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
     1141 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
     1201 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
     1261 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt ctctctgatg cgtaatggaa
     1321 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1381 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt tgcagccgga accgtccagt
     1441 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgatcttag tctatgcgct
     1501 ccattcacga aaattgcgcg gataattatt ttgtgaaggc tattatccta cacctgtaag
     1561 aaa
//
LOCUS       DAZFWP020000375         1559 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFWP020000375 DAZFWP020000000
VERSION     DAZFWP020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.378"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..1241
                     /gene="nleA"
                     /locus_tag="ACHK0Z_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             3..1241
                     /gene="nleA"
                     /locus_tag="ACHK0Z_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HGU4505092.1"
                     /translation="MNVQPNVHTGLTPPLHLAHPPHPAPNQIPESELPPGCETGFVVN
                     IPEDIQQHTPECGETTALLGLIRDKGLLSGLDAYLAPHLEEGSAGKKALDMFGLFNVT
                     QMALEIPSTVPGISGKYGVQMNIVKPDIHPTTGNYFLQLFPLHDEVGINFGALPGPLQ
                     KFLSQNPNPWFGVTAQVVRNHGIELPIVKTDNGWKLVGETPLTPEGPKANYTEEWVIR
                     PGEFDLKYGSTPLQSTLGLEFGAHFKWDLDNPNTKYAVLTNALANAAGAAGGYTLSQY
                     TGTDAMLSPHVGSMAGQALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAETGEGRF
                     TDYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGL
                     PHHNPSNHVDIFDIIQETRV"
BASE COUNT          493 a          337 c          312 g          417 t
ORIGIN      
        1 ttatgaatgt tcaaccaaat gtgcacactg gattaacccc ccccttacat cttgcacacc
       61 caccacatcc tgcgccaaat cagataccgg aatcagaatt gccacctgga tgtgagactg
      121 gatttgttgt taatattcca gaggatatac agcaacacac tccggaatgc ggtgaaacaa
      181 cagctctgtt gggtttaata agagataaag gtctgctctc agggttagac gcatatcttg
      241 ctcctcacct tgaagaaggc tcagcaggaa aaaaagcatt ggatatgttt ggtttattca
      301 atgttactca gatggcatta gaaataccca gcaccgttcc tggtatctct ggtaaatatg
      361 gtgttcagat gaacattgta aaaccagata ttcaccccac aaccgggaat tattttttac
      421 agttattccc cctacatgat gaggtgggaa taaacttcgg tgcgctccca ggaccactcc
      481 agaagtttct ctctcaaaat cccaatccat ggtttggagt aacagctcaa gttgttcgta
      541 accacggcat agaactacct atagtcaaaa cagataatgg atggaaacta gttggagaaa
      601 caccacttac acctgaaggg cctaaagcaa attatacgga agaatgggtc atcagacctg
      661 gtgagtttga cttaaagtat ggcagcactc cgttgcagag cactttaggt ttagaatttg
      721 gagctcattt taaatgggat ttagataatc ctaataccaa atatgccgtt cttaccaatg
      781 cacttgcgaa tgcagctggt gctgcagggg gatatactct atctcaatat acaggaacag
      841 acgcaatgct gagcccccat gttggctcga tggccggaca ggctttgggg catgctgtac
      901 aatgctacac tccaggatta aagccagaca ctattttatg gtgggcaggt acgactctgg
      961 ggctggcaga tttaaacaaa gccgagacag gtgaaggcag attcactgac tatcctcgta
     1021 tatggtggca cgcaagagaa ggtgccatct tccctaataa agaagatatt gcccgtgcaa
     1081 caggggctga tatacgagct atggaagaag gtatccctgt tggacagcgg catccaaatc
     1141 cagaggatgt ggtaatcgat atcgaaagca atggcttacc acatcataat ccatcaaatc
     1201 atgttgatat ctttgatata atccaagaaa caagagtcta agctttaact tgtttatttt
     1261 aaaataatag ctgaaatgga tggcagctat tattaataag gattaatata ttttttctaa
     1321 aataccccac aaaataaaca caccataaaa attctaaata cacacaccta atctcaaaaa
     1381 aacaaactta ctatttgact tggattctgt ttttaaccca atattttccg acagcatctc
     1441 cacagaaaaa tgcatttacg taaagacgcc ctcaagtcat ctgaaacctg tcaaccacag
     1501 gcatactctt gtattcttga tgcctgtggc gtttttacaa attactgaca gctaatatt
//
LOCUS       DAZFWP020000376         1557 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFWP020000376 DAZFWP020000000
VERSION     DAZFWP020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.379"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..375
                     /locus_tag="ACHK0Z_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             <1..375
                     /locus_tag="ACHK0Z_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4505093.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            650..1219
                     /locus_tag="ACHK0Z_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             650..1219
                     /locus_tag="ACHK0Z_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4505094.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            1219..1368
                     /locus_tag="ACHK0Z_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             1219..1368
                     /locus_tag="ACHK0Z_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505095.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            1376..>1557
                     /locus_tag="ACHK0Z_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             1376..>1557
                     /locus_tag="ACHK0Z_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4505096.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDV"
BASE COUNT          422 a          322 c          431 g          382 t
ORIGIN      
        1 ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa
       61 tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata
      121 aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt
      181 ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg
      241 ggagcgtgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
      301 tcaaacaact gttacggtca ggtatcaaga cgtgaccagg agagtgcgct ggcatgctgg
      361 ggtatcgaca ggtaagcaga atattttgct gaaaaatgac gttggccaac gcgggtagac
      421 aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag attgtttcat
      481 gaagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc
      541 aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt
      601 gggctggtta ttcctgtgcg cagggttcat cactaaaagg aaataaccga tgaatatgat
      661 gaccgtgccg tttcacggcg attctcttta tgtggttaac cataacggcg aaccatacgt
      721 tcccatgaaa cctgtcgttg cggggatggg gctggcctgg caatcacagt tggctaagtt
      781 aagacagcgt tttgcgtcaa ctataacgga aatcgttatg gttgctgagg atgggaaacg
      841 acgcaatatg gtgtccctgc cgcttcgaaa acttgcaggc tggttacaaa ccatcaatcc
      901 caacaaagta aaacccgaaa tccgcggcaa ggtaatccag tatcaggaag agtgtgacga
      961 tgttctctat gaatactgga cgaagggttt tgtcgttaat ccccgtcgaa tgagtgtgat
     1021 ggaagaactc aatcaggctt gcgctgacat gaaacgggat aaaaacattg ccagtgtgtt
     1081 tgctaccggg ctgaatgagt ggaaacaggt taaatccgcg catgtatcaa aaatccgcac
     1141 attgataaac gaagcgaatc tgctgattga ttttgtcctg gctgatacag acaaagggaa
     1201 aataacaaag gcggattgat ggagtggtgg ctaatgatat cggataaact cataacgctg
     1261 gcgaagatcc tctgtgtaat cgtcggcatt tcatttttag tcattctggt tgccattttc
     1321 ttttccaccg cttggcgagt cctgacgtta tcgggactgg tggggtgaaa gagagatgaa
     1381 ccgtgttctg tgtgtggtga ttattgtcct gctggtagcc tgtggtgcac ttagtctggg
     1441 gctgaatcat taccgcgata acgccataac ctacaaagag cagcgcgata aaaaagtcag
     1501 tgagctggag ctggcaaatg caaccattac tgatatgcag cagcgccagc gtgatgt
//
LOCUS       DAZFWP020000377         1551 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFWP020000377 DAZFWP020000000
VERSION     DAZFWP020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.380"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1551
                     /locus_tag="ACHK0Z_RS26420"
                     /old_locus_tag="pgaptmp_005284"
     CDS             <1..>1551
                     /locus_tag="ACHK0Z_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505097.1"
                     /translation="PLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLI
                     ALQYDISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSW
                     LTSLQNDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNE
                     QLKENLSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDE
                     LRICASCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGL
                     ILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLV
                     SGTEIQTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIR
                     EGKVPGVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISF
                     ALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFS
                     TASPTVRLA"
BASE COUNT          395 a          393 c          406 g          357 t
ORIGIN      
        1 tcccctgcag atcaaaaaca ttcagcagaa cgggatcaca ccagaagata tcagcaagga
       61 aattaacggg tattactgct gtttttatac tgcctttttc cttatggcgt gttctgcatg
      121 cgcaccattg atcgcgttac agtacgatat ttcaccgtca ctgatgtggt ggggcgggtt
      181 gttgtactgg ctggctgcat tagtgacgct gctatgggcg gccagccaaa tccaggcgct
      241 gaaaaaactg acctgtgcca tcagccagac actggaagaa caaccggtgc tcaacagtaa
      301 atcgtggctg accagtttgc aaaacgatta cagccttcct gactcactga cggagcgcat
      361 ctggctgacg ctcatttctc aacggatttc ccggggagag ctgagggaat ttgaactggc
      421 agacggaaac tggttactga acaatgcctg gtatgaaaga aacatggcag ggtttaacga
      481 acagttgaaa gagaacctgt cattcacacc tgatgaactg aaaacgctct tccggaaccg
      541 cctgaattta tcaccggaag cgaatgacga ttttctcgat cgttgcctgg acggcggtga
      601 ctggtatccc ttttcagaag gtcgccgttt tgtatcattc catcacgtgg atgagcttcg
      661 tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc ccggaaaatc ataagccgga
      721 tccggaatgg tactgctcct ctctttgtcg cgaaacagaa acactgtgtc aggaaattta
      781 tgaacgccct tacaacagct ttatttccga tgcaacggcg aatggtctga ttctcatgaa
      841 actgccggaa acctggagta caaatgagaa aatgtttgct tccggagggc agggacatgg
      901 gtttgccgct gaacggggaa accatattgt cgacagagtc cgtctgaaaa acgcacggat
      961 cctcggtgat aataatgcca gaaatggagc agacagactg gtcagcggaa cagaaatcca
     1021 gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg gcattcgacg gacaaaacgg
     1081 acagtatcgt tacatgggaa ataatggccc catgcaactg gaagtccccc gtgatcagta
     1141 tgccggcgct gttgaaacca tgaggaataa gatccgcgaa ggtaaagtac ccggtgtaac
     1201 cgatcccgca gaagcatccc ggctgattcg tcggggacat ctgacttata ctcaggcccg
     1261 taatatcacc cggttcggga ccatcgaatc ggtcacttat gatattgccg agggttcggt
     1321 tgtcagtctg gcggccggag ggatcagttt tgccctgacg gcatcggtct tctggctcag
     1381 caccggcgat cgcgatgctg ccctgcagac agctgctgtc caggcaggaa aaaccttcac
     1441 ccgcacactg gctgtctacg tcacaaccca gcaactccac cggctcagtg ttgttcaggg
     1501 tatgctgaag cacattgatt tttcgactgc cagcccgact gtccgactgg c
//
LOCUS       DAZFWP020000378         1516 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFWP020000378 DAZFWP020000000
VERSION     DAZFWP020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.381"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            526..751
                     /gene="ykgL"
                     /locus_tag="ACHK0Z_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /pseudo
     CDS             526..751
                     /gene="ykgL"
                     /locus_tag="ACHK0Z_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(786..926)
                     /gene="ykgO"
                     /locus_tag="ACHK0Z_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             complement(786..926)
                     /gene="ykgO"
                     /locus_tag="ACHK0Z_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU4505098.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(926..1189)
                     /gene="ykgM"
                     /locus_tag="ACHK0Z_RS26435"
                     /old_locus_tag="pgaptmp_005287"
     CDS             complement(926..1189)
                     /gene="ykgM"
                     /locus_tag="ACHK0Z_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4505099.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          445 a          305 c          327 g          439 t
ORIGIN      
        1 tcataaggtt attggcaata taacgttcaa ttcttacttg cattttatat tagccaggaa
       61 ctttgccctg aggtgttagg aatagtctta atgtgttgtg taatggacta gtaaaaaaac
      121 aagtttaaat gataagaaat agaatataaa actatcatga gtcataatta aagatgattt
      181 gccaatcagt aaggtgtttt aatggattaa catgccgcac aaaatggtta tgggtgttca
      241 tcccaataag tgatgcaaaa ttaaacaggt ataagtacgt catctgtccg gtaagccccc
      301 taagagccaa attaagtgaa gttaaacgaa gactgccagt tgtcacagga acgatggcct
      361 ctttgccagg gcactgcatt cagtcatctg tgcgcgttac tctaactggc actcgaacgt
      421 cggcgatggg gttaccggtg gatcaggccg ttcctgtggc gtaaacgttt ttatgtcagt
      481 accaacagat ttaccacatt tatcatctta acagtttgag tgtaaatgca ggctgcgtcg
      541 caaagggagg aaactgagtg gcgtgttcag tctaaaagag ggttgatgcc agcatacagg
      601 gcgaagctgg tcaacaagtc aacattaata taatggaata ttcggagaga aacgtcaggc
      661 agttgacctt aaatgaacag gaagatacat ccccagaaaa attaatgctg gtgtaataaa
      721 tacaccaaca ttaatccggt ctgattatta atcagacaaa tatcgttaat tcgtcgaatt
      781 ttaaatcaac gttttttctt acgaccctga acggccttaa aacgtggatt agatttacaa
      841 atcacataca gccgtccttt tcgcttcaca atctgacagt ctggatggcg ttcttttgcg
      901 gtacgcagag agttaaggac tttcatcatg cccccttttt cgtgctaaca aaacgaccaa
      961 aacgttgggt gaaccgtgca acatttcctt ctgatgccac tgttctcagc ttccctgtat
     1021 agaacgggtg cgatttcgaa gagacatcaa ttgtcacgta tggatacgtt acgccatcca
     1081 gctcaatctc acggtctgtt ttgatagtcg agccgatttt aaagtactca tcaacactgg
     1141 tgtcgtggaa caccacagta cgatactcag gatggatatt gggcttcatc atttttacct
     1201 gttatgttat aacataacca taaagataca tgctcatccg gatcatttca acacccaatc
     1261 cctgtgccca tttttggtat gtggaatttc cccaacgggg aagtctggca ttgttagcag
     1321 ccgttggcga cgctcccata aacgcctgga tgagtgattg atgatggttt cgctgagagt
     1381 atggattttt cgacaatgga ggttatctcc actttactgg ccgatgattt atcgcagtat
     1441 gattgattac ctgacagcgt gtattcctct cccacggata taataattac ttacaaaaag
     1501 ggagaggatg catatt
//
LOCUS       DAZFWP020000379         1509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFWP020000379 DAZFWP020000000
VERSION     DAZFWP020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.382"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1509
                     /gene="rhsB"
                     /locus_tag="ACHK0Z_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             <1..>1509
                     /gene="rhsB"
                     /locus_tag="ACHK0Z_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HGU4505100.1"
                     /translation="FTDCSGYQTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVK
                     DTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRV
                     IRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDE
                     ADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHH
                     PQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTR
                     DRLHRETLRRFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTR
                     SYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDA
                     HYLYRYDRYGRLTEKTDLIPEGGIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESR
                     YLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSR"
BASE COUNT          352 a          477 c          457 g          223 t
ORIGIN      
        1 cttcactgac tgttccggct accagacccg ttatgaccat gaccgcttcg ggcagatgac
       61 ggcggtgcac cgcgaggaag ggctgagcca gtaccgcgca tacgacagcc gtggacagtt
      121 aattgccgtg aaagacacgc agggccatga aacgcggtat gaatacaaca tcgccggtga
      181 cctgaccgcc gtcattgccc cggacggcag cagaaacggg acacagtacg atgcgtgggg
      241 aaaggccgtc cgtaccacgc agggcgggct gacgcgcagt atggaatacg atgctgccgg
      301 acgggtcatc cgcctgacca gtgaaaacgg cagccacacc accttccgtt acgatgtact
      361 tgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc accacgacct
      421 gaccggcaaa cttatccgca gcgaggatga gggtctggtc acccactggc actatgacga
      481 agcagaccgc ctcacgcacc gcaccgtgaa gggtgaaacc gcagagcggt ggcggtatga
      541 cgaacgtggc tggctgacag acatcagcca tatcagcgaa gggcaccggg tgacggtgca
      601 ttacgggtat gatgagaaag gccggctgac cggtgagcgt cagacggtgc atcacccgca
      661 gacggaagca ctgctctggc agcatgagac cagacatgcg tacaacgcgc aggggctggc
      721 gaaccgctgt ataccggaca gcctgcccgc cgtggaatgg ctgacctacg gcagcggtta
      781 cctggcaggc atgaaactcg gcgacacacc gctggtggag tacacccgcg accgcctgca
      841 ccgggaaacg ctgcgccgct tcggccgtta tgaactcacc accgcttata cccctgccgg
      901 gcagttacag agccagcacc tgaacagcct gctgtctgac cgcgattaca cctggaacga
      961 caacggcgaa ctcatccgca tcagcagccc gcgccagacc cggagttaca gctacagcac
     1021 caccggcagg ctgaccggcg ttcacaccac cgcagcgaac cttgatatcc gcatcccgta
     1081 tgccacagac ccggcaggta accgcctgcc cgacccggag ctgcacccgg acagcaccct
     1141 cagcatgtgg ccggataacc gtatcgcccg tgacgcgcac tatctttacc ggtatgaccg
     1201 ttacggcagg ctgacggaga aaaccgacct catcccggaa gggggtatcc gtacggatga
     1261 tgagcgcacc caccggtacc attacgacag tcagcaccgg ctggtgcact acacgcggac
     1321 acaatatgca gagccgctgg tcgaaagtcg ctatctttac gacccgctgg gccgcagggt
     1381 ggcaaaacgg gtgtggcggc gtgaacggga cctgacgggc tggatgtcgc tgtcacggaa
     1441 accgcaagtg acctggtacg gctgggacgg cgaccggctg accacgatac agaacgacag
     1501 gagccgcat
//
LOCUS       DAZFWP020000380         1477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFWP020000380 DAZFWP020000000
VERSION     DAZFWP020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.383"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(36..770)
                     /locus_tag="ACHK0Z_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /pseudo
     CDS             complement(36..770)
                     /locus_tag="ACHK0Z_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            complement(767..1098)
                     /gene="sctI"
                     /locus_tag="ACHK0Z_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /pseudo
     CDS             complement(767..1098)
                     /gene="sctI"
                     /locus_tag="ACHK0Z_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1118..1358)
                     /locus_tag="ACHK0Z_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /pseudo
     CDS             complement(1118..1358)
                     /locus_tag="ACHK0Z_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
BASE COUNT          437 a          301 c          248 g          491 t
ORIGIN      
        1 tttcttaggg caagattcat aacgctctca tatgtctact taattttcaa cctgactaaa
       61 ttagttagaa tggccctata cttccataac agccagcaag tcgctacgga tattaatgca
      121 agtaagatag aaaccggcat agccttatca taagcaaaaa caggttcgct aatttcatat
      181 gttggtgctt gctcaataat gtctcttcgt tttgacaata caacagaaat attttcatat
      241 tgtacgcttg cagagctatt aacaataaat ctcttgatat catttatttt tatttctggg
      301 ttgatatctt tttcatatac tgcaagtaca gaaatatgaa ttggtaaagc agttttacca
      361 ctatcgccat tatcaacatc gtaactaaca tgtactctcg aagaaataat gccatccata
      421 attttgagag attgctctaa ccgctgctca atagcagaat atagccttgc tttttccgct
      481 cgtggagatg aaacgagtgc atctgcaggg aacatctgcg atatttgaat atcaggctta
      541 cccggtagat tgtagatttt tagccaatcc accgcagaag caaaatccgt tggttcgaca
      601 aatattgaaa atcctgtttt gccttaatcc ttcttttcag cattaatatt atgtctttgt
      661 aaaacagcaa ggacatcatt agcttgctgt tgatcaagat ggttcaataa ttcctgctgc
      721 ttgcagccgc acaacagcag gataaacaat aataatgata tatatttcat gagcgggtca
      781 acgtctcaac tgcagcgaca atctttcttg ctaatgttcc accaatgaaa cctcgacatt
      841 ataattaagc acatgatcct gcaagactaa taatttttca ggagttaacc cctctttatc
      901 agaccttaat gcttgtaacg cattttgctg ccaattttca gtttgtacag catagttggc
      961 aaaagcggag atcattttat catccgggtt gtcaagattg agaacccggt catttaaatc
     1021 aatcactttc gcttgctgta tttcatgagc agttgattgc tcagctctat caataggtgg
     1081 catattgctt actgacataa taaaatcctt atccagttta gcggaagttt tgtatgattg
     1141 ctgaatcaag atctttatac gcttttacaa cgttagattg cgcatttcga tataatgtat
     1201 actcagctaa tgcactttga tattcggcaa gaaatttcgt gtcggaggga ttggttggct
     1261 aaatcctcca acgctttttc aacttgctgg ttcagatcat caacgccctg atcaaattga
     1321 ttgctgatat ccataatata accattccaa tctgccataa gtcctcctca attattcctg
     1381 aaatgtccag ttgctacctt gagacttctc gaatcggtta tctccgtagc taaatgaact
     1441 attatttgtg tcctgatttt tcaaattcac attaaat
//
LOCUS       DAZFWP020000381         1470 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFWP020000381 DAZFWP020000000
VERSION     DAZFWP020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.390"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..203
                     /locus_tag="ACHK0Z_RS26460"
                     /old_locus_tag="pgaptmp_005292"
     CDS             <1..203
                     /locus_tag="ACHK0Z_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4505101.1"
                     /translation="IAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRAQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            200..1021
                     /locus_tag="ACHK0Z_RS26465"
                     /old_locus_tag="pgaptmp_005293"
     CDS             200..1021
                     /locus_tag="ACHK0Z_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4505102.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1259..1470
                     /locus_tag="ACHK0Z_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /pseudo
     CDS             1259..1470
                     /locus_tag="ACHK0Z_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
BASE COUNT          392 a          289 c          419 g          370 t
ORIGIN      
        1 ttattgccga gccaccggat aagcgccgcc gtgacttgga caatattctg aaagcgccgc
       61 tggatgcgct gacgcatgcg gggttgctaa tggacgatga gcagtttgat gaaatcaata
      121 tcgttcgtgc tcagccagta tctggtggac gtctgggggt gaagatttac cccataatgc
      181 ttgaagggca ggtcaaaaaa tgaaactgga agatttaccg aaatactact ccccaaaatc
      241 ccccggcctg actgatgcat cggcctcaac gtcgaaagat acgctgagta tcactgatgt
      301 gatggctgcg cagggcatga cacagaattg ggctgagatg gggttttctg cgttccttgg
      361 gaaaatgggc attagtatga atgacagaga gcgggcaaca gaattgctga cagaatatgc
      421 actcagtcgg tgtgatcgcg tggcggcgtt aagaaaactc ccggcagaaa taaaaccggc
      481 agtgatgcgt attatggctt cgtatgcgtt tgaagattat gcccgtagcg cggcgagcaa
      541 aaaacagtgc ccctgttgtc acggaaaaaa atttattgaa agcgaggttt ttacaaacaa
      601 gatccagtat ccggatggta agccgccagt gtgggcaaag tgcacaaaag gcgtgtatcc
      661 gtcttactgg gaggaatgga aaaaagtcag ggaggtggta aaagttgcct gtccggagtg
      721 tggagggaag ggggaggttt ccaccgcctg taaagattgt cgtgggcgcg gtgttgccat
      781 tcatcgtgaa gagtcggtaa aacgtggtat gcctgttatc agagactgcc agcgttgtgg
      841 tggtcgtggc tatgaaagat taccttcaac ggaggcattt aatgccatat gtaatgtaac
      901 cgatgccata tctcttgata catggaaaaa aacagttaaa cgtttttacg atacgctggt
      961 ggtgcagttt gatattgaag aagcatgggc agaacaacaa ctgaaaaagg tgaccagata
     1021 gctttgttga tttttcccga atctgtggta aatttgccct aacgatgggc gttttatgcc
     1081 tgacgttaga agatttttta cacccgtcgc caggcgggtt tttttatgac tgaaatcacg
     1141 ccagtacagt aaacgcgctg gtggttgtga ataccggtct ttcagcttgc tggctttttc
     1201 gacaagagtt attggtgtgt cacgttaacc ggaaaaagga aagtttgaga aacgcgatct
     1261 ggcacaggcg gttattaatg ctgcctacct ggtggcctgt gcagatggtg aatgtgaggc
     1321 ttcctagaaa gcgaagatcg aacaggtact gcgtaatcag cctgcgctgt ccgcgtttac
     1381 gtcagaaatt aatgcgatta gcgcaaccat tatcggtcag ctggatacga actttaaaat
     1441 tggtcgtcgt gcggcgttac gtgagatcga
//
LOCUS       DAZFWP020000382         1425 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFWP020000382 DAZFWP020000000
VERSION     DAZFWP020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.391"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(102..755)
                     /gene="ribB"
                     /locus_tag="ACHK0Z_RS26475"
                     /old_locus_tag="pgaptmp_005295"
     CDS             complement(102..755)
                     /gene="ribB"
                     /locus_tag="ACHK0Z_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU4505103.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(859..1007)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1129..1419
                     /gene="ubiK"
                     /locus_tag="ACHK0Z_RS26480"
                     /old_locus_tag="pgaptmp_005296"
     CDS             1129..1419
                     /gene="ubiK"
                     /locus_tag="ACHK0Z_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU4505104.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          394 a          379 c          335 g          317 t
ORIGIN      
        1 aagagaagcg gaattaacat cttgcatagc ggggcaaaat aaaaaccgaa gccagctaca
       61 acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca
      121 tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg
      181 gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac
      241 agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc ttcagtatga
      301 ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga
      361 ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca
      421 gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg
      481 ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct
      541 tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca
      601 acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca
      661 agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa
      721 ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat
      781 ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg
      841 gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa
      901 ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga
      961 gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa
     1021 cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct
     1081 tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat gattgacccg
     1141 aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag
     1201 ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc
     1261 gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag
     1321 aaactggcgc tgcttgaaca aagaatgagc gaactggaaa atcgcagtac tgagattaaa
     1381 aagcagccag atccagagac tctcccccca acgctgtaaa cattt
//
LOCUS       DAZFWP020000383         1418 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFWP020000383 DAZFWP020000000
VERSION     DAZFWP020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.392"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..577
                     /locus_tag="ACHK0Z_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             <1..577
                     /locus_tag="ACHK0Z_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HGU4505105.1"
                     /translation="TEQTGKLLNRKITTTPTPAKLLSYRNADLIKENYITEKVLSIFN
                     IKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVMDWYNPQKNAFGIMMGTPRRSADIA
                     KEESRNALNFMIMEKDTFNEKILNSNDNLQKKYGTTENSSWVSASVGSLLDKGAKVYP
                     DISCSLRLGEPFIITLPETVRLDVDIHPLKK"
     gene            complement(590..1288)
                     /locus_tag="ACHK0Z_RS26490"
                     /old_locus_tag="pgaptmp_005298"
     CDS             complement(590..1288)
                     /locus_tag="ACHK0Z_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505106.1"
                     /translation="MNEIRSNPVKFLEEHLILNPQRYAHHDETSLITVNITREDGLLR
                     LKETEDDMAGSDYILFTGMRDTDSPDRFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQSYSMIHMRPRDSNDFVDEFTPALYANTQEILLER
                     DIQRTLANTFPNEHPEALILVPSAEKFIVDGNSIQLIGIGNELGEFDFYRQIYPVTGG
                     EHLVEALMWTHLPA"
BASE COUNT          483 a          301 c          246 g          388 t
ORIGIN      
        1 aacagagcaa acaggaaaac tgttaaacag aaaaataacc accactccca caccagcgaa
       61 actattatca tatcgaaatg cagatttaat aaaagaaaac tacattacag aaaaagtttt
      121 atctattttc aatataaaac gggatttcgt tgctgtaaga atccaaagca atcagtttac
      181 tgatttaaaa aacaagacaa ttcagggtca taaagacact gtcgcaaaag tcatggactg
      241 gtataaccca caaaaaaacg catttggtat catgatgggc acaccaagac gttctgctga
      301 tatagcaaag gaagaatcca gaaacgctct aaatttcatg ataatggaaa aggatacatt
      361 taatgaaaag atacttaaca gcaatgataa tttacaaaaa aaatacggca caacagaaaa
      421 tagtagttgg gtatcggctt ctgttggttc tcttctggac aaaggagcca aagtatatcc
      481 agatatatca tgctctctga gactgggaga accattcatt attacacttc cagaaacagt
      541 caggctggat gttgatatcc acccactcaa aaaatgaacc tgaacgtttt catgcaggca
      601 aatgcgtcca catcaaagct tcaaccaggt gctctcctcc tgtcacagga taaatttgtc
      661 tgtaaaaatc aaactctccc aactcattac caataccaat cagttgaatg ctgttgccat
      721 ctacaataaa tttttccgct gacggaacca aaattaaggc ttccggatgt tcattaggaa
      781 aggtgttagc aagagtacgc tgaatatccc tctccagcaa aatctcctga gtattagcgt
      841 aaagcgctgg cgtaaactca tcgacgaaat cgttactatc ccttgggcgc atatgtatca
      901 ttgaatacga ctgttctcca gattctgata atccatggcg aacaataaga acactacagc
      961 cgctttgctg attggttagc cataacggtg aactttgctg gatagtttga gaaatatcat
     1021 ttcgccggag gcttatacac tgattactca agcttacaac tgcaggtgca tcaaagcggt
     1081 ccggactatc agtatcacgc atacctgtaa acagaatata atcactacca gccatatcat
     1141 cttccgtttc ttttagtctt aacaagccat cctctcttgt aatattaact gtaattaaag
     1201 atgtctcatc atgatgggca tagcgctgtg gattaagaat taaatgctct tccagaaatt
     1261 ttacagggtt actacgtatt tcattcatta tacaccgaag ctctgaacga tgcggcacca
     1321 gaaagtgccc ggtattttgt gaaataatac tacttacctt gattgttttc tctccagctc
     1381 atgaaaaata actcgcgtat tatattgttt taccaacc
//
LOCUS       DAZFWP020000384         1405 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFWP020000384 DAZFWP020000000
VERSION     DAZFWP020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.393"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..878)
                     /locus_tag="ACHK0Z_RS26495"
                     /old_locus_tag="pgaptmp_005299"
     CDS             complement(<1..878)
                     /locus_tag="ACHK0Z_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4505107.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEVARHAEEAKKNAGEAETSARNAGISASQAEEGAANADTSAGEASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNAARDATTA
                     TEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGD
                     TGPAGATGERGPRGDTG"
     gene            complement(943..>1405)
                     /locus_tag="ACHK0Z_RS26500"
                     /old_locus_tag="pgaptmp_005300"
     CDS             complement(943..>1405)
                     /locus_tag="ACHK0Z_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4505108.1"
                     /translation="NLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHED
                     SVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGS
                     DDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          247 a          471 c          340 g          347 t
ORIGIN      
        1 ccagtatctc ctctcggccc cctttcaccg gttgcccctg ccgggccggt gtcgccacgc
       61 tctcctttat ctcccttcgg cccctgagga cccgcgggac ccggttcccc ctttggcccg
      121 ggaggcccca ccacggtggg gattcggttt acggcctctt ccgccgctat cctgctttgt
      181 tccgctgact gtgcgctttc tgctgactcc cgggcttttt ctgttgcggt cgttgcatcc
      241 ctggctgcat taccggctgc actttctgcc gtctttcttg acaattcagc ttctgctgca
      301 ctttgtgatg actcactggc tttttgagcg gccgcagagg ccgaggacga ggacgcttcc
      361 tctgactgct ttgcagcggc tgcactttct gccgcctgcc gggctgactc cgatgcctcc
      421 cctgctgaag tgtcagcatt tgcagcgccc tcttctgcct gactggctga tatgccggca
      481 ttcctcgctg acgtctccgc ctctccggca ttcttcttcg cctcctctgc gtgacgcgcc
      541 acctcttcca ccatcagttc aaaacggcgc agtgcctccg gccggacgtc atcctccgac
      601 atggcaccga gaaaatcatt cagcgtcccc ggttgagaat cttcatacac ggtgatggtc
      661 ccggcatgtg acggcgggaa gccctccacc aacagaatga cgctgtactg accgtactca
      721 acgtccatgc tgtaacgacc ggcttcatcc ggattttcag aggccaccgt attcaccacc
      781 accgtgctgc tggtccgtct ggctttcagt tgaatggtgc agttctctac cggttttcct
      841 gtgccgtctt tcagtacacc tgaaatcttt actgccatat tcaccccaca aaaaagcccg
      901 cctgaaccgg cgggctgtca taacactgtg ttacctggct aatcagaatt tatagccgac
      961 acccacgatg aagccgtcag tgcgccagtc gccactgccg gagccttcat aagcaatatc
     1021 aatggccacg gattcggtcg ggttaaactg cacgccagct ccccacgcca gagacgtgtt
     1081 gctgtggcga ccgtcatcac ttccggtcag cacatcgtgc gttttcccct tgttgtcagt
     1141 tacgcgaaga taatccccgg agaaagtcga cacacggctg taagccacac ccgccatcgc
     1201 atacgcgctg aaccattcat tcacgcgcac agacggcccc gccattacgc tgaaccagcg
     1261 gttacgcacg gaatcttcat gccagcgggt atcgctgtaa cgggtaagct ggcgattctt
     1321 gtctcctgca tagctgaatg acgtcaccag ccccagcgtg tccgtgaatt cataacggta
     1381 tttcacgtta atcccgttaa gatta
//
LOCUS       DAZFWP020000385         1400 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFWP020000385 DAZFWP020000000
VERSION     DAZFWP020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.394"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..565)
                     /locus_tag="ACHK0Z_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             complement(<1..565)
                     /locus_tag="ACHK0Z_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505109.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLG"
     gene            684..1400
                     /locus_tag="ACHK0Z_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /pseudo
     CDS             684..1400
                     /locus_tag="ACHK0Z_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          449 a          280 c          237 g          434 t
ORIGIN      
        1 ttccaagttt tggaggtttg ataggcgttt taatttcact gcgcaacgca tctaagtttg
       61 taagatcact gtctataatt ttaagatagt ttaaatctaa cgaaccatca gttttatcaa
      121 gcttagtttt cagcctttta acagcctcca aggaagcatc aagaactggt gtctgattct
      181 tcaaagcttc tgaaaaactt ttttgtgttg attttcgagc ttgttgttga gcagattttc
      241 ttaactcttt ttcacgctta acttgttcta aaagctcttt acggtcatca gaacgtgaac
      301 caaaaaatct atcagcaagt tcagttaaca aattagatat aatagttttc agttctcttg
      361 cagcctggtt tcttatgaat ccctctctcc ccgacttatc tttcagctct ttattactgg
      421 attgagtaat tccaatataa ccaaaaatcc ttctattaga ccaataatat cgccctgcat
      481 tccatgaacg tctttcttct atctggaaga aatcattatc tactcgacca taaggtaata
      541 ctctcaagct atccctaaaa atcattagtc ctgcatactt tttggcctta agatcaaagt
      601 gggaatgttc acgttcagta tgtgatgtat tttgtgaaag gaattcaaac gttcctatct
      661 gaagctcaaa tgggccgacc cctgaatggg aaaacctcca tgcattaaaa gaaaagcgtt
      721 taaatggaat gcctaaaaca acccccaaaa gtgaccaggt tattatgctg caaaatcaat
      781 acgttcgtga aatgcgaaaa tatggcgtac gaggtttacg ttacgatgca gcaaaacact
      841 caaaacatga acaaatagaa agatcaataa ccccaccgct taaaaattat aatgagcggt
      901 tacacaatac taatttattt aacccaaaat atcatgaaaa agtcgttatg aattacatgg
      961 aatatctggt aacttgtcag ttggatgaag aacaaatgtc atcgctgctt tatgaaagag
     1021 atgatttaag cgccattgat ttttcattgc tcatgaagac gataaaagct ttttcatttg
     1081 gtggcgatct ccaaaccctt gcatcaaaac cgggttccac aatctcaagc atcccatcaa
     1141 aaagacggat attgattaac attaaacacg attttcctaa caatggcaat attttcaatg
     1201 actttctatt taaccatcaa caagatgaac aattagcaat ggcatatatg gccgctctcc
     1261 cgttcagcag gcctttagtt tactgggatg gccaagtatt aaaatcaacg actgaaatta
     1321 aaaattatga tgggtcgacg cgtgttggcg atgaggcgtg gcttaataaa ggttgctcta
     1381 cctatcagca gctctacaat
//
LOCUS       DAZFWP020000386         1389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFWP020000386 DAZFWP020000000
VERSION     DAZFWP020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.395"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..792
                     /locus_tag="ACHK0Z_RS26515"
                     /old_locus_tag="pgaptmp_005303"
     CDS             <1..792
                     /locus_tag="ACHK0Z_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HGU4505110.1"
                     /translation="SGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTE
                     LAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQ
                     VWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPA
                     GLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYR
                     FKSRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            743..>1389
                     /gene="traT"
                     /locus_tag="ACHK0Z_RS26520"
                     /old_locus_tag="pgaptmp_005304"
     CDS             743..>1389
                     /gene="traT"
                     /locus_tag="ACHK0Z_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU4505111.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFE"
BASE COUNT          399 a          332 c          369 g          289 t
ORIGIN      
        1 agcggttatt acaactgggt acagcatgaa ccctcagaca gaaaacaaag tgatgagcgg
       61 ctaaaactgg agattaaggt ggcacatatc cgcactcgcg aaacatatgg aacccggcgg
      121 ctccagacgg agctggcaga gaatggcatc atcgttggtc gtgaccgact ggcacgtctt
      181 cgtaaggagc taaggctacg ctgtaagcag aaacgcaagt tcagggcgac tacgaactcg
      241 aaccacaatc tgccagttgc gccaaatctg ctgaaccaga cgttcgctcc tacagcacca
      301 aatcaggtct gggtggcgga cctgacgtat gttgccacac aggagggatg gttgtacctc
      361 gctggcatca aagatgttta tacgtgcgaa attgtcggct acgccatggg agagcgcatg
      421 acaaaagagc tgacaggtaa agccctgttt atggcgctca ggagccagcg cccacctgcc
      481 gggctaatcc accactctga tcgaggttca cagtactgcg catacgatta ccgggtcata
      541 caggagcagt ttggtctgaa aacatcaatg tcgcgtaaag gtaactgtta cgacaacgct
      601 ccgatggaaa gcttctgggg aacgctgaaa aatgagagcc tgagccacta tcgttttaag
      661 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
      721 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      781 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgtcagtaca cctcaaccat
      841 ctggcttgaa cctgcgagcg aacgcaccgt tttcctgcag atcaaaaaca cgtcggataa
      901 agacatgagt ggactacagg gaaaaattgc cgatgcagtc aaagccagag gttatcagat
      961 catgacttcc ccggataaag cttactactg gatccaggct aacgttctca aagcagacaa
     1021 aatggatctg cgtgaatctc agggatggct gagtcgcggt tatgaaggtg cagcagtcgg
     1081 agctgctctt ggaggtggaa tcacagcgta caataccggg tcggcaggta ccactctcgg
     1141 tgttggactg gctaccggtc tgattggcat ggccgcggat gcaatggtgg aagatataaa
     1201 ttacacaatg attacagatg ttcagattgc cgagcgaacc agaaccagcg tcagaacgga
     1261 taatgttgca gctttgcgtc agggaacatc aggctccaaa attcagacaa gcacagaaac
     1321 cggcaaccag cataaatacc agacccgggt tgtatccagt gccaaccagg ttaatctgaa
     1381 gtttgaaga
//
LOCUS       DAZFWP020000387         1374 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFWP020000387 DAZFWP020000000
VERSION     DAZFWP020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.396"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..825
                     /locus_tag="ACHK0Z_RS26525"
                     /old_locus_tag="pgaptmp_005305"
     CDS             <1..825
                     /locus_tag="ACHK0Z_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4505112.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPY
                     PIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYL
                     PQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            822..>1374
                     /gene="ybfC"
                     /locus_tag="ACHK0Z_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             822..>1374
                     /gene="ybfC"
                     /locus_tag="ACHK0Z_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGU4505113.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIEIQHELI"
BASE COUNT          437 a          283 c          323 g          331 t
ORIGIN      
        1 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
       61 taccactgcg accatcgcgg cctgccgctg gcgcttgtca gcacggaagg ggcaacagaa
      121 tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      181 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      241 cgccaccgct attatgaccc gctgcagggg aggtatatca ctcaggatcc gattgggctg
      301 aaggggggat ggaattttta tcagtatccg ctgaatccgg ttcagtatat agattcaatg
      361 ggactggcat caaaatatgg acacttaaat aatggcggat atggagcgag acccaacaaa
      421 ccgcctacgc ccgatccaag taaattgccg gacatagcga aacaattaag actgccatat
      481 cctattgacc aggccagtag tgcgcctaat gttttcaaaa cattcttcag agcattaagc
      541 ccttacgact acacactgta ttgcaggaag tgggtaaaac caaatctgac ttgtacgcca
      601 caggatgatt cccagtatcc agggatggat acaaagacag caagtgatta cctgccacag
      661 acaaattggc caacaactca attaccacca ggatatactt gtgcagaacc ctatttattc
      721 ccagacatta ataaacccga tgggccagca acagcaggga tagatgattt gggtgaaatt
      781 ttagctaaga tgaaacagag aacatcgaga ggaataagaa aatgaaaaga gttttgttct
      841 ttttgctgat gatatttgtt agttttggtg ttatagctga ttgcgaaata caagctaaag
      901 atcatgattg ttttactatt ttcgctaagg gtactatttt ttccgcgttt cctgtcttaa
      961 ataataaagc catgtggaga tggtatcaga atgaggacat cggtgagtat tattggcaaa
     1021 cagaactggg tacatgtaaa aataataaat tcacaccaag cggggcgaga ctcttaattc
     1081 gcgtcgggtc actacgcctt aatgaaaatc atgccatcaa aggaacgttg caggagttga
     1141 taaatacagc agaaaaaacg gcgtttctcg gcgatcggtt tagaagttat ataagagcgg
     1201 gtatctatca aaaaaagagc agtgatccag ttcagttgtt ggcagtcctt gataattcaa
     1261 tcatggtaaa atattttaaa gatgagaaac caacttatgc tcgaatgaca gcacatctcc
     1321 cgaacaaaaa tgagtcttac gaatgtttaa tagagataca gcatgaacta attc
//
LOCUS       DAZFWP020000388         1360 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFWP020000388 DAZFWP020000000
VERSION     DAZFWP020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.397"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            36..1181
                     /locus_tag="ACHK0Z_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             36..1181
                     /locus_tag="ACHK0Z_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HGU4505114.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPFSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          464 a          278 c          244 g          374 t
ORIGIN      
        1 aatcattcat aataaatgag ataggggaat ctctcatgat aaacggactt aataataatt
       61 ccgcatcttt agttttagat gctgcaatta gaattaattc tgattttaaa aagcagtgga
      121 atgatatgtc atgcgctgaa aaattactaa aagttcttag ttttggttta tggaatccaa
      181 cgtatactcg tagtgaaagg caaacatttc aagagttgtt aaccgtttta gaacctgtat
      241 ctcccgcgcc taatgaattg ggcagaatat atgctaattt tgcagatggc tcatccttaa
      301 gaatttccgt cactaacagc gaacttgttg aagccgagat tcgcacacca gataatgaaa
      361 agattcttat gctcctggag tcaaacgaac aaaataggtt attacaatct ttacccatca
      421 atctccacat gccatacatt caggttcatc gtgccttatc taagatggac ctgactgatc
      481 ataagtcaat gcacaatcta cttagtttta cgtcaaaact atcagcaacc ttgattcctc
      541 ataatactca aacagatccg ttttccgggc ctacaccatt cagctctatg tttatggata
      601 catttcgagg actcggcaat gcaaagcttt cactcaatgg tgttgatata cctgtagatg
      661 cacaaaaatt gcttcgcgat gcactaggac ttaaagacac acattcatca cttgcccgga
      721 atgttataaa taatggtatt tctcgtcatc atgcagagca aatagcaagg gaaagcagcg
      781 gcagcgataa acagaaagct gaagttgtgg agtttttatg ccaccccgaa gcggcaacag
      841 ccatatgctc ggctttctat caatctttca atgtgccagc cttaatgttg acacatacaa
      901 ggatctctca agccagagaa tacaatgtgg aaagatcgtt agatgtacct aacgcgtgca
      961 ttaacataag tatctctcaa tcaccagatg gaagcattca cgttgccagc cataccggga
     1021 ttctgataat ggcgccagaa gaccgcccca acgagttagg aatgttgacg aacaggacct
     1081 cctatgaagt gccacaaggt gtgaaatgtg aaatcgatga gatggtaaga acgctacaac
     1141 caaggtatgg cgcatctgaa acatacctga aaaacattta aaaataaaaa aatggacacc
     1201 acacacttta tttatcaaaa caaacaataa ctaatcacac tcacaattat taataattta
     1261 acctcacagc aatcatacaa aacaaagata tttatttcgc aaaccattta tagcgaatgg
     1321 agaaggaatg aaaagaagct tatttattgc attttttatt
//
LOCUS       DAZFWP020000389         1343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFWP020000389 DAZFWP020000000
VERSION     DAZFWP020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.398"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            211..348
                     /locus_tag="ACHK0Z_RS26540"
                     /old_locus_tag="pgaptmp_005308"
     CDS             211..348
                     /locus_tag="ACHK0Z_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505115.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            350..844
                     /locus_tag="ACHK0Z_RS26545"
                     /old_locus_tag="pgaptmp_005309"
     CDS             350..844
                     /locus_tag="ACHK0Z_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4505116.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQVEGAQDYIRTQCTKQAFYY
                     PEDV"
     gene            841..1065
                     /locus_tag="ACHK0Z_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     CDS             841..1065
                     /locus_tag="ACHK0Z_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505117.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
BASE COUNT          357 a          294 c          370 g          322 t
ORIGIN      
        1 tcacggcgtg accaggagag cgcgctggcg tgctggggaa ttgacagata agcagaatat
       61 tttgctgaaa aatgcggttt gctcacacgg acggataaca caaaatcctg cgaactgaca
      121 aaaactaagt gaataaaagt aaaaacccca tttgttggct gcaagcgggg ttttgtgttt
      181 cctgactccg gaaaagtcaa aggagaaagt gtgtttgatt ttagcaaact gattcgggag
      241 attcgagtga tggctgaaaa attatcaacc tggaagtaca tccttatctg gctggtgttt
      301 gtgattatgg cctccggtta tttcatcggt cagatacgct ggtggtgaaa tgaaccgcgt
      361 actgtgcgtg gtcatcattg ccctgctggt ggcctgtggt gcgcttagtc tggggctgaa
      421 tcattaccgc gataacgcca tcacctacaa agcgcagcgc gataaaaaag ccagagagct
      481 ggagctggca aacgcaacta ttactgatat gcaggtgcgc cagcgcgatg ttgctgcgct
      541 cgatgcaaaa tactcgaggg aattagccga tgcgagagct gaaaatgaaa ctctgcgcgc
      601 tgatgttgcc gctggtcgta agcgcctgcg gatcaacgcc acctgccccg gtaccgtgcg
      661 tgaagccacc ggcacctccg gcgtggataa tgcaaccggc ccccgactgg cagacaccgc
      721 tgaacgggat tatttcatcc tcagagaacg gttgatgaca atgcagaagc aggtggaagg
      781 ggcacaggac tatatccgca ctcagtgtac taagcaggct ttttattatc cggaggatgt
      841 atgaagaaat tacaggtaac ggtaaaacct tttcagggaa caattccgtt ccgtgttttg
      901 caacatggcc gtgttctgct tgaagaggtg ttcagaggta aatgcactga atgttattca
      961 cgaacatatg aagtgaatgc cacgcatgaa gaattcaccg ttgagtgtgt gatgaatact
     1021 gataaatgcc gaatggtatc cgctgaatta cagccagtgt gttgagcgac cttattatcc
     1081 atgcgcggta ttgtcgccgt attcctgcat taacagagac cgcagcccga cagggagact
     1141 cctctgcgag agtgtgcggg gataatcaaa aacgatacac accggggttt accgcgtaaa
     1201 cggagcgcgg cgttctcccc tcatggtcgc ccgtccggtg cgatggtgga agaaactgga
     1261 atctgttcaa taaaaaaact gccgtgttgg agtcacagca gtaatgtact gattgggtag
     1321 aagattatta ttgttatgct tta
//
LOCUS       DAZFWP020000390         1343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFWP020000390 DAZFWP020000000
VERSION     DAZFWP020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.399"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..731)
                     /locus_tag="ACHK0Z_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     CDS             complement(<1..731)
                     /locus_tag="ACHK0Z_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4505118.1"
                     /translation="MWNLLRRTRKNQKSGRDVREVGWRSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQN
                     RIQFFELWLNSKLRHGNTVVLKIRNPRGQIKELRILDWNRVEPLVADDGDVFYRITPD
                     RNCGITESVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAAMQGHHIQANSTYFFRNG
                     GRPSGVIEVPGSITEENAKKLKGNW"
     gene            complement(677..898)
                     /locus_tag="ACHK0Z_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     CDS             complement(677..898)
                     /locus_tag="ACHK0Z_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505119.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(943..>1343)
                     /locus_tag="ACHK0Z_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             complement(943..>1343)
                     /locus_tag="ACHK0Z_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4505120.1"
                     /translation="LMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVG
                     DQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAI
                     RAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          275 a          446 c          340 g          282 t
ORIGIN      
        1 tcccagttcc ccttcagttt tttcgcgttt tcttccgtaa tactgccggg gacctcaatc
       61 acgcctgatg gtcgcccgcc atttctgaaa aaatacgtcg aatttgcctg aatatgatgc
      121 ccctgcatgg cggccagtcc tgcggcatac accggcggca gccccacaag cggatgaaaa
      181 aaacagttaa accggtcgtg gatcacctcc cgggcaggca ccgtcaccga ctctgtgatc
      241 ccacagttcc ggtccggcgt gatgcggtag aacacgtcgc cgtcatccgc caccagaggt
      301 tcaacccggt tccagtccag aatacgcagt tctttgatct gcccccgggg gttgcggatt
      361 ttcagcacca ccgtattgcc gtgacgcaat ttggaattca gccacagttc aaaaaactgg
      421 atgcgattct gctgcgcatt aggacgacga cagagacggg cagtatctcc ctgccgcttt
      481 tcccggcgta ttccctgtac atcagtctgc atcaggcgaa gccgcatttt ggcaatatcc
      541 tgggatatca gcgaaatgca tgaaaacacc gcatgaaagg agaggacact ttccggatcg
      601 gctttcacac cctgctgcca ggcaccggca aaaggctcag ccaccgcctg aaacagggac
      661 cgccagccca cttctcttac gtcacgtcct gatttctggt tttttcgggt tcgccgcaaa
      721 aggttccaca ttcgccatgc tccgcatcac gtttcttttt ctgacctgcc ggacgtcgca
      781 ctgtgatgta ctccgccttc cccaggcgaa ccagcacctc cgcacacggc tgtgccacat
      841 cacggatatc cccggcccgg gcatcatgcg tgccctgcag atatcggatc tttgccataa
      901 cctgttacgg gaggcgcacg cctcccgtcc tcctcatcag actcagccgc cggacgcact
      961 gccgtagttc actccggtga tcaccgccac cgccgcggta cggcgacgac gccagttgat
     1021 ccagcgctcc gcacggatgg ccacgctgcc tgtctggaac atggaaacca gctccaccgg
     1081 ggacggcgtg gtactgtcgc cggtcggctc agactgcatt tccagtgatg cctcgcggga
     1141 catatccact gccacgccgc cgtcatccgc cagataaata tccggggcat tcaccagcac
     1201 cagctggtca cccacgtact gggagacaat caccggcagc ccctggaagg agccacccag
     1261 cagggtcatg tccgggtatt ccttctgacc cagcgcattt ttacgcatgg acagtgccag
     1321 ggcattggtg ctggacatca gcc
//
LOCUS       DAZFWP020000391         1336 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFWP020000391 DAZFWP020000000
VERSION     DAZFWP020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.400"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..321
                     /locus_tag="ACHK0Z_RS26570"
                     /old_locus_tag="pgaptmp_005314"
     CDS             <1..321
                     /locus_tag="ACHK0Z_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4505121.1"
                     /translation="FERWKAIDTRDKREKFTALIPAIMEAIRISDFRLYREITDGKSI
                     TYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKRAYRHQYQQKAGWNYE
                     PSFC"
     gene            302..1267
                     /locus_tag="ACHK0Z_RS26575"
                     /old_locus_tag="pgaptmp_005315"
     CDS             302..1267
                     /locus_tag="ACHK0Z_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505122.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTSSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKALGLLRSEKLNKSKRDMTNFYTINY
                     DSALLDDGKVNESIGSKCATPSGQNDTMEEVKMKRSIGSKRPNVIRSKWPDDPTENTT
                     ENTTENKKLSCPDASQPDELTAEQAFLTRHPDAVVFSVKKRQWGTREDLTCAQWIWGR
                     VVNLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFSRAQRDPFWI
                     KNIKSPEKLREKWDELVIRLWRGPAQRCVNHISEPDTEIPPGFRG"
BASE COUNT          386 a          282 c          370 g          298 t
ORIGIN      
        1 ttcgaacgct ggaaggctat cgacaccagg gataagcgtg aaaaattcac ggcgctgatt
       61 ccggcaatta tggaggctat ccggattagt gatttcaggt tgtaccgcga aattactgac
      121 ggaaaaagca ttacgtacat gattgccggg ttaaacaaag aatatggaga tgtggtggag
      181 tccgggctgc tttttgcaga tccagctgtt gtggaacgtg agacagacga gcttatagaa
      241 aaagcaatcg ctttcaagcg tgcctatcgt caccagtacc aacaaaaagc cggatggaat
      301 tatgagcctt ctttttgctg aacgcccgct ggttataaac acacagctgg cgatgaagat
      361 tggtttaaac gaagccattg tgttgcagca actgcattac tggttgaggg ataccagttc
      421 cggcatggaa tgtgatggcg ttcgctggat ttacaacaca acggaacaat ggctggaaca
      481 gtttccgttc tggtcagagt caacgttaaa gcgtgcgttt gcaagtctga aagcgctggg
      541 gcttttgcgt agcgaaaagc tcaataaatc gaagcgtgat atgactaatt tttacacaat
      601 taactacgat agcgcgcttt tagatgatgg caaagtgaac gaatccatcg ggtcaaaatg
      661 cgccactcca tcaggtcaaa atgacacgat ggaagaggtc aaaatgaaac gttccattgg
      721 ttcaaaacgg cccaatgtca tcaggtcaaa atggcccgat gatcctacag agaatacaac
      781 agagaataca acagagaata aaaaactctc ttgtccggac gcttcgcaac cggacgagtt
      841 gacggctgaa caggcgtttt taacccgcca tcctgatgca gttgtgttca gcgtgaaaaa
      901 acgccagtgg ggaacccggg aggatttgac atgtgcgcag tggatctggg ggcgggttgt
      961 aaacctgtac gaacaggctg ccagtgacga tggtgagatc acgcgaccga aagagcccaa
     1021 ctggacggcg tgggccaatg acgtgcgcac aatgcggatg ctggatggaa gaagtcacag
     1081 acaaatttgc gaaatgttca gccgggcaca acgggatccg ttctggataa aaaacatcaa
     1141 gagcccggaa aaactccgtg agaaatggga tgaactggtt attcgactgt ggcgtggtcc
     1201 cgcgcagcgt tgcgtgaatc atatttctga accggatacc gaaattccgc cgggtttcag
     1261 ggggtagcgc atcatgaaaa acattacgtc aggtggtgtt ctggcaagag tcagcagatt
     1321 tgtgccgcag gatgca
//
LOCUS       DAZFWP020000392         1328 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFWP020000392 DAZFWP020000000
VERSION     DAZFWP020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.401"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..234
                     /gene="etgA"
                     /locus_tag="ACHK0Z_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             <1..234
                     /gene="etgA"
                     /locus_tag="ACHK0Z_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HGU4505123.1"
                     /translation="KFNISEDDLLNDACINISVAGYILASNIKSRGNTWDAVGAYNAG
                     YFNTPNAVELRRQYAMKIYKTYTKLKNNEQIID"
     gene            complement(231..1268)
                     /gene="escU"
                     /locus_tag="ACHK0Z_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             complement(231..1268)
                     /gene="escU"
                     /locus_tag="ACHK0Z_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HGU4505124.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQTGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLIFYFMGHSY
                     ANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKD
                     EVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGE
                     TPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARSLYKNIHKGQYITEDFFEPVA
                     QLIRIAIDLDY"
     gene            complement(1261..>1328)
                     /locus_tag="ACHK0Z_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     CDS             complement(1261..>1328)
                     /locus_tag="ACHK0Z_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505125.1"
                     /translation="FITANIHSDIIDRSLPSMINE"
BASE COUNT          449 a          245 c          208 g          426 t
ORIGIN      
        1 aaatttaata tatctgaaga tgatttattg aatgatgcct gtataaacat atctgttgct
       61 ggttatatct tagcctctaa cataaaatcc agggggaata catgggatgc agttggtgcg
      121 tataatgcag gctactttaa cacaccaaat gcagtagaac tcagaagaca atatgcaatg
      181 aaaatatata aaacatacac taaattaaaa aacaatgaac aaattatcga ttaataatca
      241 agatctattg ctatacgaat caattgtgct acaggttcaa aaaagtcttc tgttatgtat
      301 tgtcctttat gtatgttctt ataaagactt cttgccaaag gaatatcttc aatcactgga
      361 atatcataga gttcagccag tttaatgatc tgtagagctt tggcatcttt tcctgtttca
      421 ataactaaag gcaatggagt ctccccgagt ttatagtata ggcaaatcgc aatgtgagtc
      481 gggtttttga cgatcacggt tgattttttg atgttattag ccagacttcc actttgtatc
      541 tcagaatgaa ggcgacggcg ctccccttta atttcagggt taccatcagt atccttggct
      601 tctcgtttca cctcatcttt actcattttc attttcttca gtccctcatg cttctggaac
      661 cagaaatcaa atactgaaaa gagtagatat ccgaataaca cgcctttcca taaaagaaaa
      721 acaaaaaagg caacaacgac aagagtttga tatgcgttca gtcttgtgaa atttgcaaac
      781 tcatttgcat atgaatgccc cataaagtaa aaaatgagag taattatcac taacttaaat
      841 actgacttaa gcagctcaaa aatactcttt acagaaaaaa tatttttcag gttatttttt
      901 acactaatct tctgagccga tggttttatt ttttcaaccg caaagatgaa tcctgtttgt
      961 gatacaccag ttgtaacagt tccaacaaag acaatcaaag aaattggcaa aatatacaat
     1021 aaaaatatgt ttaataccgc acctaatatt tcacgaatgg catacaaaaa cggtctattt
     1081 agcgagtcta tagtcgtatt aactaactca acaacctcaa cgaaaaaact cgcaccatat
     1141 aaagaaaaaa atgaaaataa gattaatgac tgcactgcag ccattacctc ttcacttttt
     1201 gttacatcgc ccttcttttt tagatccctt agttttttgg gtgtgggctt ttctgttttt
     1261 tcactcatta atcatgctcg gtaacgatct gtcaattatg tcagaatgaa tatttgccgt
     1321 aataaaat
//
LOCUS       DAZFWP020000393         1317 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFWP020000393 DAZFWP020000000
VERSION     DAZFWP020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.402"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..595
                     /locus_tag="ACHK0Z_RS26595"
                     /old_locus_tag="pgaptmp_005319"
     CDS             <1..595
                     /locus_tag="ACHK0Z_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGU4505126.1"
                     /translation="GRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGAC
                     TNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTA
                     PNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIK
                     SFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            567..950
                     /gene="yhhH"
                     /locus_tag="ACHK0Z_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             567..950
                     /gene="yhhH"
                     /locus_tag="ACHK0Z_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HGU4505127.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1055..>1317
                     /locus_tag="ACHK0Z_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             1055..>1317
                     /locus_tag="ACHK0Z_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HGU4505128.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKC"
BASE COUNT          476 a          189 c          294 g          358 t
ORIGIN      
        1 ggggcgatat atcactcagg atccgattgg actgaagggg ggatggaacc tgtatggata
       61 tcaattgaat ccgatatcag acatcgaccc cctgggttta tctatgtggg aggatgcaaa
      121 atcgggggca tgtactaatg gtctttgcgg cacactatcc gctatgatag gtccagataa
      181 atttgattct atagatagca ccgcatatga cgccttaaat aaaataaata gccaatctat
      241 ttgcgaagat aaagagttcg ctggtttaat atgtaaggat aatagtggca gatatttctc
      301 aacagcacct aaccgaggag aaagaaaagg atcatatcca ttcaatagcc cttgccctaa
      361 tggtactgag aaagtatcag cttatcatac tcatggtgca gatagtcatg gagaatattg
      421 ggacgaaata ttttcaggta aagatgagaa aatagttaaa agtaaagata acaatatcaa
      481 gtcattttat ttaggtacgc ccagtggtaa ttttaaagca atagataacc acgggaagga
      541 aataacaaac agaaaaggat tacctaatgt ctgcagagtt catggtaata tgtaaaaaaa
      601 tattgtttag gaactgtgtc attgtatctt tgtttgtttt tacatacaac acatgggcgc
      661 agtgtaataa taatattaaa ataatgcgca agtatgaaag tgaaggtaaa tataccgtta
      721 gaaatttggt taaaaataaa gctatagcat tggaattagc tgagatatat gttaagaatc
      781 gttatggaca ggatgccgca gaagaagaaa aaccatacga aatcactgag ttaacaacaa
      841 gttgggttgt tgaaggtacc attcactcag accaaattgc tggtggggtt tttattatag
      901 aaataggcaa aaatgatggg agaattctga attttggcca cggaaaataa gtatgtaagg
      961 ataatcaaac atcgtgtgcg ctgatggcag agtaggtgga ggactccaga cagtcaaacg
     1021 atagaaaaag atagccttta tggaggttcc tgcaatgtca aatacatacc agaaaagaaa
     1081 ggcaagtaaa gagtatggtt tatataatca atgtaagaaa ctaaatgatg atgaattatt
     1141 tcgcttactt gatgatcaca attccttgaa aaggatttca tctgccagag tattacagtt
     1201 aagaggtggg caagacgctg ttagattggc aattgagttc tgctctgata aaaattatat
     1261 ccgtagagat atcggagcat ttatactcgg gcaaataaaa atttgcaaaa aatgcga
//
LOCUS       DAZFWP020000394         1312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFWP020000394 DAZFWP020000000
VERSION     DAZFWP020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.403"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..583
                     /locus_tag="ACHK0Z_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /pseudo
     CDS             1..583
                     /locus_tag="ACHK0Z_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            complement(808..>1312)
                     /gene="nleG7"
                     /locus_tag="ACHK0Z_RS26615"
                     /old_locus_tag="pgaptmp_005323"
     CDS             complement(808..>1312)
                     /gene="nleG7"
                     /locus_tag="ACHK0Z_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HGU4505129.1"
                     /translation="NGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDF
                     MLQLSSNIGWKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKN
                     ALNSNICTLYDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDAD
                     QQKQHCK"
BASE COUNT          399 a          249 c          266 g          398 t
ORIGIN      
        1 caaataaaag ccatctacaa tcaaataata gcgatgttgt tgctgctgca gctataagta
       61 ttacagcgac agagatgaaa accatcctgc cagatgattt aacaccagga aaatacaaaa
      121 aatttatctg tctggggatg gttctgctgg tctaccactt cttaagtgtg gagatgaatt
      181 tttatcgccg acagatattg tcgaccgcat tgttcaacat aatcttcatg agattgatga
      241 tatcagatta acatcctgta actcagccaa catattaaaa aacaaagact tctctcctga
      301 tgaaatagag aaatccgcaa atatgaataa cggctggttg gccagggcat tatttggtca
      361 aaagagttct ttagcagaac acgtctatgc cgagtttgaa cgtcgcggaa ttaacgtttc
      421 tatatcaggt taccatggca ctggcgtttt ttatgtacca gagcatggta aaccaacaac
      481 acatctacgc tccacaactg tgcctgcaac acctgaacat actgtaagaa gaagcgacta
      541 cagagccact ttgggtagaa ctcaacccat agatattgag taattgagcc agcatataca
      601 cttgatggtt aggaaacaga atcattgttt taaacatttc ctggccatta atattgtcgg
      661 cgggagcata tccaggactg gccggcaaac cgggtacgcg atctgttgcc atggaaagtt
      721 gatctgagcg ctcagtaaat atcaatacgg ttctggcgag ccgcttacga tgcatgagaa
      781 tcactaatag tatgtaatta ttacatttta tttacaatgc tgtttctgtt ggtctgcatc
      841 cataattatg aaatgctgac ttatggtgtc aaaataacac atattccttt caataatcat
      901 ttcttttact atcttttccc tgctgagagg gtggggtaga tgttctcttg tgagattcat
      961 gaacgcagac ttatcataaa gagtgcatat gttggagttc agtgcgttct tgacaaaaac
     1021 ccctttttct ggaacgcata atgttattgg gcacttaaga aattgtgtgt cgcaagagaa
     1081 ttcatcagga tttacaacaa atgaatgaga atggatttta tttttaaggg cgttttcggc
     1141 cccttttttc catcctatat ttgaagataa ttgaagcata aaatcctgag atatttgtgc
     1201 aaaacttcgt ccgttgttga tctgctgttc caggttgcga gcaatagtgt tttgtctcgc
     1261 cataaatatg gagaaatgat tactgcatac aggatttact gtaaaaccat tg
//
LOCUS       DAZFWP020000395         1308 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFWP020000395 DAZFWP020000000
VERSION     DAZFWP020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.404"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            481..690
                     /locus_tag="ACHK0Z_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             481..690
                     /locus_tag="ACHK0Z_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505130.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(691..>1308)
                     /locus_tag="ACHK0Z_RS26625"
                     /old_locus_tag="pgaptmp_005325"
     CDS             complement(691..>1308)
                     /locus_tag="ACHK0Z_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505131.1"
                     /translation="PSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRDAFTGLYF
                     CASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPYAGNMFVY
                     DNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAEKRWNVAT
                     WLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
BASE COUNT          356 a          313 c          269 g          370 t
ORIGIN      
        1 attcccgtta cgcccgtaaa agaccgcttg ttttaactgt tccgcgtgcg cttagccgca
       61 ttcaccgcat cacaaaattc actttaaaaa gggcggcaga gcagccacgg agtaaaactg
      121 ataccgccaa acgtcaccag aaaattgata acagagggcg ttgcagcggg gttgtcactt
      181 aagcgtattg tcaacctgac aacccggtgt cctcaacggg gaaggaataa ccccgccata
      241 cttaccgccg cgccatttcg cggagtgcca caaccggaag cgcacgttcg aagaaatcta
      301 acgacaagcc ttctaaggga aagagcttcg ccgtacgctt tcgcgttatg ccctgacttt
      361 tcagggaaat atcctttcag taaactgtca gtaccggatt cttatccgtg tccggcgcac
      421 gaccacacgt gacagcgtgt tggtctccat ttttaaccca gaacctcaat ggaggataaa
      481 atgccaaaca aaaaaagaaa tccgcttatt gaaaaacaga ttgaatgcct ggtaaatcaa
      541 ctcaggcaat cagggttatt aaaaactcat tcagagttga ggctcacaga atcagcattc
      601 gacgataaat taaataatgt cctttataat ggcattattg attttaatcg ttctgttggt
      661 cgccgcggcc ctgctggtgt ttccttataa ttaccagtca atccagagtg gaccgtgttc
      721 agcgtaaata taactgtaca catccagatt atatttgtgg tctgttaaga acaggccgca
      781 aatacatgcc gaagcttcca gtgcagcggc tctgttactg aataaccatg tagcaacatt
      841 ccagcgtttt tctgcatccc agtctttctc aaggcctgat accatgaaga aaccgttagt
      901 gttgccatca aataattctg tttccaaatt tttaagcaat gcctgatgga ctcttgccag
      961 gtattccgcc ggaatttcgc cacgaattct gatgagattg tcataaacaa acatgttccc
     1021 cgcatatggc gatttttctt tcttgttttt taaaccagca tcatgagcaa actgatcaat
     1081 ttcttcttcc gttggtttcg tattgatgtt ttgcgctgtc gtttctgcaa ttttatttgc
     1141 cacactctct gagtcgtgtt tatttataga cgcacagaaa tacaatccgg taaacgcatc
     1201 gcgcacatta cgagccatat tatcagtgtc ttttttcgtt accgattcca attcaagttc
     1261 gttcagacga tgacgaagtg tgtgtgctgc aatctcctgg attgaagg
//
LOCUS       DAZFWP020000396         1303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFWP020000396 DAZFWP020000000
VERSION     DAZFWP020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.405"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1226
                     /locus_tag="ACHK0Z_RS26630"
                     /old_locus_tag="pgaptmp_005326"
     CDS             <1..1226
                     /locus_tag="ACHK0Z_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4505132.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKENTLALGVYPE
                     VSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRY
                     AVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYKGQPVTRLAVMLN
                     LLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQA
                     VAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQEVCGHGFRAMAC
                     SALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREKF
                     ISPFEYAKINNPLKQ"
BASE COUNT          366 a          311 c          301 g          325 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggtt catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa ggagaatact ctggcacttg
      121 gtgtgtatcc agaagtttct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcaga ggggattgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc ttttgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaggta ctcaaaagcc tcgaaacaca cgttttcccc tttatcggca
      361 accgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aatatctgct gtaatgcgtt
      481 atgctgtaca gtctggcatc atcagatata acccggctct ggatatggct ggtgcattga
      541 ccacggtaaa acgccagcat cgccctgctc tggatctttc acgcctgcct gaacttctgt
      601 cgcgtattga cggttataaa ggccagcctg tcacccgact ggcagttatg ctgaatttac
      661 tggtttttat tcgttccagt gaactcagat acgcccgctg gtctgaaatt gatattgaca
      721 atgccatgtg gactattcca gccgaacgcg aacctctgcc aggcgtaaaa ttctcacacc
      781 ggggctccaa gatgcgaaca ccacatcttg tgccactcag caaacaggct gtagccatac
      841 tgacagaact tcagacatgg gcaggtgaaa atggtctgat atttacgggt gcacatgacc
      901 cgcgtaaacc aatcagtgaa aatactgtaa ataaagccct gagggtgatg gggtatgaca
      961 caacccagga agtctgtggc catggattcc gggcgatggc gtgcagtgca ttgattgaat
     1021 caggtttgtg gtcccgcgat gctgtggaac gtcagatgag ccatcaggaa cgtaacggtg
     1081 tacgtgctgc ttacattcat aaagcagaac atctggaaga acgccgcttg atgctacaat
     1141 ggtgggccga ttttctggat gcaaacagag aaaaatttat cagtccattt gaatatgcaa
     1201 agattaataa tccattaaaa cagtaatcat cccgggcaaa tgcccgggaa cctactccag
     1261 gattattttc tttgttaaaa aaacagacaa aacggtatta act
//
LOCUS       DAZFWP020000397         1297 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFWP020000397 DAZFWP020000000
VERSION     DAZFWP020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.406"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..90)
                     /locus_tag="ACHK0Z_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             complement(<1..90)
                     /locus_tag="ACHK0Z_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505133.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFL"
     gene            171..1297
                     /locus_tag="ACHK0Z_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /pseudo
     CDS             171..1297
                     /locus_tag="ACHK0Z_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          476 a          180 c          304 g          337 t
ORIGIN      
        1 cagaaagaga aaaatataaa atacatacag aaagacatga gagcaaggga cggggcaggg
       61 caagatcatg aaaattgtga tgtaaatcac tattttcatc ttggctttaa cgcctacagg
      121 tgatcaaaat ttaacctttg gaataactaa aaagataaaa aaggacccag gtgagtattc
      181 aaagtttgct tgattatatt tcagtgatcc ctgatatacg acaacaagga aaggttaaac
      241 ataaattatc tgctattttg tttctcaccg tatgtgcagt aattgcaggt gccgatgagt
      301 ggcaggaaat tgaagatttt ggacatgaaa gacttgaatg gctaaagaaa tatggtgatt
      361 ttgataatgg cattccggtc gatgacacca ttgcacgcgt tgtgagtaac attgacagtt
      421 tggcctttga aaagatgttt attgaatgga tgcaggagtg ccatgaaatc actgatggtg
      481 aaattatagc aatagatgga aagaccataa gaggctcctt tgataaggga aaaagaaaag
      541 gagcaatcca tatggtgagt gcattctcga acgaaaatgg tgttgtactg gggcaggtga
      601 aaacggaagc caaaagtaat gagattacag ccattccaga gttgcttaac ctactgtatt
      661 taaagaaaaa tttgataacc attgatgcta tgggctgtca gaaagatatc gcttcgaaga
      721 tcaaagataa aaaagcagat tatcttctgg cagtaaaagg caatcagggg aaattacatc
      781 atgcattcga ggaaaaattt cctgtaaatg tgttttctaa ttataaaggc gattcgttta
      841 gtacgcagga gataagtcat ggaagaaaag aaacacgttt gcatattgtc agtaacgtaa
      901 cgcctgaact tttgtgattt tgaattcgaa tggaagggat taaaaaagct ttgtgtagca
      961 ttgtcattca ggcagaagaa agaagataaa tcagcagaag gtgtaagcat ccgatattat
     1021 atttcatcaa aggatatgga tgctaaagaa tttgcacatg ctatcagagc gcactggctg
     1081 atcgagcaca gtcttcattg ggtgttagat gtaaaaatga atgaagatgc cagccggata
     1141 agaagaggaa acgcagccta aataatatct ggaataaaga agatggcact gaatttatta
     1201 agagattgca aagacattaa gggtgaagag gaaaagaaag aaggctgcgt taaacacagg
     1261 gaaagatcat cagaagttca ctttttgtac taaataa
//
LOCUS       DAZFWP020000398         1282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFWP020000398 DAZFWP020000000
VERSION     DAZFWP020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.407"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            617..907
                     /locus_tag="ACHK0Z_RS26645"
                     /old_locus_tag="pgaptmp_005329"
     CDS             617..907
                     /locus_tag="ACHK0Z_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4505134.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            931..1182
                     /locus_tag="ACHK0Z_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             931..1182
                     /locus_tag="ACHK0Z_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4505135.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
BASE COUNT          356 a          232 c          301 g          393 t
ORIGIN      
        1 gtaataagcc acatttattc tttcgggaat taaaaaacat aacgaaattt tgcctcattc
       61 tgcatttcat tgaaatagta tgtttctcat cgaaagcaag tttgtcaata taaatttgta
      121 acagaaatga ctttccgggg ataaacaaaa ctgttcatgg caatggcttt ctctgtcgcc
      181 aacatggata cattgttccg gataaagcac tgatgtcatt gactgacgca gactatcagg
      241 ccatctgatg ctttcttttc cttcaccggt attgatgata tgccttatga agggggactt
      301 attcctgtaa cccaatcaca ggcactgtga ttactggttt atgattgatg ctgatttgag
      361 cagatatgca cagcagttgt ggattcaggg gatattttct gccagatatt cataccagat
      421 tgatgacctc tttgctcaga tgctcttcgt tctgagccag ccaggatttt gtggccacat
      481 acattgttcg ccgatgggga atcgagacag gcgattggct gtgatgcgat tttgccgcta
      541 actgaggatg agtccgcccc tcccggggaa ttcctgtaag ttaagccgta cctgttgatt
      601 agcactaaac tgggtaatga atttatctgc cagtatgaat gatgccgggg acggttttga
      661 actcctgatg tcgggtaata tgcaggattt taatatttgt tgtcgtcggg ataatcatta
      721 tcctgaacat aaactgatac ctttatcaga gttatttgac gttaattatg gactgaatct
      781 tgagctgaat aaactggaga aagattcttc aggaattaat tttgtttcaa gaacgtccaa
      841 aaataatggc gtctccgcca gggttaaatt aaaagatggt attagtccat gccagcagct
      901 gtgttaactg tagcagcagg aggttctgtt atggaatcgt ttgtacagga ttcaccattt
      961 tatagtggaa gagatcttta ttggttacgt cctaaagttg aactgactct ggaagagaaa
     1021 ttgtactact gctcttgtat aagaagaaac agacacaaat acagctacgg gcgtcaggca
     1081 aaccgaacgc tgaagaattt actggtaccg tcattagatt ctgttccggc atgggtatat
     1141 ggtgtgacag ggaaaataat atctgaattg tctgaacgtt gagactaaga gggccggagg
     1201 ccaggtgaat ggccaccggg atatttatat cagagtatat tggcgattga ttttgccagc
     1261 tggtcttcca gatgaggttt ag
//
LOCUS       DAZFWP020000399         1279 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFWP020000399 DAZFWP020000000
VERSION     DAZFWP020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.408"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..884)
                     /locus_tag="ACHK0Z_RS26655"
                     /old_locus_tag="pgaptmp_005331"
     CDS             complement(<1..884)
                     /locus_tag="ACHK0Z_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4505136.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVSRAAGGAGALSDWLLRHVKSCQWLHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQG"
     gene            complement(886..1164)
                     /locus_tag="ACHK0Z_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             complement(886..1164)
                     /locus_tag="ACHK0Z_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505137.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
BASE COUNT          276 a          340 c          364 g          299 t
ORIGIN      
        1 ccctgaccat gaccaatcag gtgatgggga tcgtcggctg gcttaccaca acacgcacac
       61 ggctgtgtct tcacccagcg agtgtatttc tcgttaaccc agcggcgacg tttaggtcgt
      121 ttcatgaaag attccggaga ctcaggatca acggcaatgc tgaccaccgt cttttcctgt
      181 ggtgggttct gttgctggtg ggcgtgaggc agcggcgcaa gattttttgt gcgctgtttc
      241 agtatgctgg tggcggtctg ctctcccggt acgatgtcgc tttcacggta catggagcgg
      301 attttttccg cacgcaaccc cagcgaacga cgtaataccg cttccggtag cgcgtccgcc
      361 acctgattgc ggaccgccca ccaggataat tcagccagag ataattcacg ctcctgcgta
      421 ccgcttattg cgtgaccgat gacgtcaatc atccatgctg acaggttttg atgagcaagt
      481 tgctcgagtg attcggatgt ctggtcacgc agctggttgt cgcagtgcca gcacaacacc
      541 attgcgccgg taccgtaacg gtgaatgacg gtttcgctgt gatgataatc accgtgtagc
      601 cactggcagg atttcacgtg acgtaataac cagtcagaca gtgcaccagc gccacccgca
      661 gcacggctca cccgctcatc gctgaaaaat ggcagtagtg atttatcttc cgccagcggc
      721 tggtgaacgg caggtacaac cccggacggc agagctcgca tgctttttgg ttccggctcc
      781 accagtattc tgccgttatg gaatgctgac attgattcac ggcctggctt aacgataacc
      841 agaccgagtt ccggtaccag aacaggtcga agtaataccc gcacattacc tccagatccg
      901 ttgctggaat gtgcgggacg gacgcggtgg ccgttcggag taagggagcc tgacggagat
      961 tatccagtga cggtagtcga ggctaagggc ttttttaacc tcgcatccgc gcctgcggta
     1021 acactgaatg agccattcgg cctgttcttc agtgcatggg ggatgctggt accagtcaga
     1081 tttgaatgcg tgaaaacacc gtccgcgcct gctggcaaag acggcagaat catcagaatt
     1141 gtataatttg gtatcgtgcg ccatcggttg tctctgctgg cgcagcaggt gccagttgtt
     1201 caggctggcg tgcgaattgt aaaccagaat gccaggaaaa aacaaaaccc gccgaagcgg
     1261 gttaagtgcg ggtgcgttg
//
LOCUS       DAZFWP020000400         1267 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFWP020000400 DAZFWP020000000
VERSION     DAZFWP020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.409"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1267
                     /locus_tag="ACHK0Z_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             <1..>1267
                     /locus_tag="ACHK0Z_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4505138.1"
                     /translation="STAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGM
                     GVARKDLKAMADDGKLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWV
                     GGANEASGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLV
                     TAARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERL
                     NRNIAARTAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARE
                     SARQYALTIDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAG
                     YQYVLANPGWTTGNGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDV
                     LAGLEDRRVALIRQQAAEQNKVYQSMLVM"
BASE COUNT          295 a          313 c          432 g          227 t
ORIGIN      
        1 ccagtacggc tgaggcgggt tcggtgatca cccagttcag ccaggcgctg gcacagggtg
       61 tgttgcgcgg tgaggaattt aattcggtca atgaaagtgg tgaccggatc attcgtgcac
      121 tggctgcagg catgggcgtg gcccgtaaag atctgaaggc gatggcggac gacggcaaac
      181 tgacggcgga taaagtcgtg cccgcgttaa tcagccagct ggggacattg cgtgatgaat
      241 atgccgccat gccagaaacg gtctctgacg ggatcacaaa ggtggaaaac gcctttatgg
      301 cctgggtggg tggtgcgaat gaggccagcg gggtgacaaa aacgctctcc ggcgtgctga
      361 acggtgttgc cggacagatt gataatgtgg caacagccgt gggggcactg gttgccgtcg
      421 gggttgcccg gtactttggc aatatggcct ccggagcgat gtctgccacg gcaggacttg
      481 tgacggctgc acgtaatgaa gttgcactgg cggaagcaca gttcagggga acgcagattg
      541 ccacggcgcg ggcaagagca gccgtgtacc gtgctcagca ggccgtggcg gcagcccgcg
      601 ggacggagat gcagattgca gcagaggccc gtctggcggc cacacaggaa cgcctgaaca
      661 gaaatattgc tgccagaacc gccgcccaga atgcgctgaa cagtacaacg gcggtgggct
      721 cacgtctgat gagcggtgcg ctgggactgg ttggaggcgt acccggactg gtgatgctgg
      781 gagctgccgc atggtacacg ctgtaccaga atcaggaaca ggccagggag tctgcgcgcc
      841 agtatgcact gacgatagat gaaatcgcgc ataaaacgcc atcaatgtct ctgcctgaag
      901 cctcagataa cgaagggcga acacgggagg cgctgacaga gcaaaaccgg ctgattgatg
      961 agcaggccag ccgggtgaaa tccctgcagg aaaaaatcgc cgggtatcag tatgtgctgg
     1021 ctaatccggg ctggacgacc ggtaacggct tcatgataaa ccatctgacc tcggtgaaga
     1081 ccgtaacgga agggcttgct caggcaacag agcagcttgc cgttgagcag tcccgtctgg
     1141 cacagatgca ggaaaaagcg cagtccattc aggatgtgct tgccgggctg gaagaccgtc
     1201 gtgtggcgtt aattcgtcag caggcggcag agcagaataa ggtgtaccag tccatgctgg
     1261 ttatgaa
//
LOCUS       DAZFWP020000401         1267 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFWP020000401 DAZFWP020000000
VERSION     DAZFWP020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.410"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            131..733
                     /gene="ais"
                     /locus_tag="ACHK0Z_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             131..733
                     /gene="ais"
                     /locus_tag="ACHK0Z_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU4505139.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNY"
     gene            complement(772..1197)
                     /gene="nudI"
                     /locus_tag="ACHK0Z_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             complement(772..1197)
                     /gene="nudI"
                     /locus_tag="ACHK0Z_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4505140.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEIMPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVAARKTLRLKGLL"
BASE COUNT          353 a          282 c          269 g          363 t
ORIGIN      
        1 ggcaaaattt tgaggcattt tgccgacatc gtaggatttt taatattaca ccaactgcta
       61 attatcgcca gaaatgtcgc tcaattttac ggtaattgtc tggttgcgct tgtctatagg
      121 tggagtttac gtgttagctt tttgccgctc atcattgaag tcaaaaaaat atttcatcat
      181 tttactggcg ctcgctgcaa ttgccggact gggtactcat gccgcctgga gtagcaatgg
      241 tttgccacgt atcgacaata aaacattggc cagactggca cagcagcacc cggttgtcgt
      301 tttgtttcgc catgctgaac gttgcgatcg ttcaaccaat caatgcttgt cagataaaac
      361 aggtattacg gttaaaggta cccaggatgc ccgtgaactg ggcaacgctt ttagcgctga
      421 catccctgat ttcgatcttt attccagtaa taccgtccgg accattcagt cggcgacctg
      481 gttttccgcg ggtaaaaagc tgacggtaga taaacgactt cttcagtgcg gtaatgagat
      541 ttatagtgca attaaggact tacaaagcaa agcgcctgat aaaaatatcg ttattttcac
      601 ccataatcat tgcctgacat atattgctaa aaataagcgt gatgcgacat ttaaacctga
      661 ttatctggat ggtttagtca tgcatgtgga aaaaggcaaa gtttatctgg atggggaatt
      721 cgttaactac taaattttca atctgacagc cagtgatggc tgtcatcatt gttaaagaag
      781 acctttcaaa cgtaacgttt ttcgggcggc aacatttaaa tcataatgca ccagatcttc
      841 aggttttacc cacgcgtagt cctgaaactc ttcgtttatt ttcacttctc ggttggcaga
      901 aacgcagtca aaaatcaggt aaatcatata aatctcttcc ttgcgaccat ctgcatacgt
      961 cttggtgcga atatcatcgc tgaaggtcca cggcatgatt tctgtcaaaa gcagctgttc
     1021 tcccagttct tcgcgaattt cgcggcgtag tgcctcttca attcgttcgc cagactccac
     1081 gccgccaccc gaaagcgccc attgaccggg gaaaacgccg cgatcgtcgg ccattttaca
     1141 cagcaaataa gcaccatcat tttgaatcaa agggcataca atagtccgtt gtcgcatagg
     1201 ggtattcctt ataaataaaa agaaaccatg agaatacggt aactcagaaa tatgtgaaag
     1261 tgacagt
//
LOCUS       DAZFWP020000402         1258 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFWP020000402 DAZFWP020000000
VERSION     DAZFWP020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.411"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..306)
                     /locus_tag="ACHK0Z_RS26680"
                     /old_locus_tag="pgaptmp_005336"
     CDS             complement(<1..306)
                     /locus_tag="ACHK0Z_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HGU4505141.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSFDRLKSREITFQQYRENLAKAGVFR
                     "
     gene            592..798
                     /gene="ybcW"
                     /locus_tag="ACHK0Z_RS26685"
                     /old_locus_tag="pgaptmp_005337"
     CDS             592..798
                     /gene="ybcW"
                     /locus_tag="ACHK0Z_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGU4505142.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNCTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(963..1157)
                     /locus_tag="ACHK0Z_RS26690"
                     /old_locus_tag="pgaptmp_005338"
     CDS             complement(963..1157)
                     /locus_tag="ACHK0Z_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4505143.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKIAYTSIKSDEE"
BASE COUNT          352 a          222 c          263 g          421 t
ORIGIN      
        1 tcggaaaact cctgctttag caagattttc cctgtattgc tgaaatgtga tttctcttga
       61 tttcaaccta tcaaaggacg tttctataag atgcgtattt cttgagaatt taacatttac
      121 aaccttttta agtcctttta ttaacacagt gttatcgttt tctaacacaa tgtgaatatt
      181 atctgtggct agatagtaaa tataatgtga gacattgtga cgttttagtt cagaataaaa
      241 caattcacag tttaaatctt ttcgcacttg atcgaatatt tctttaaaaa tggcaacctg
      301 agccattggt aaaaccttcc atgtgatacg agggcgcgta gtttgcatta tcgtttttat
      361 cgtttcaatc tggtctgacc tctttgtgtt ttgttgatga tttatgtcaa atattaggaa
      421 tgttttcaat taatagtatt ggttgtgtaa caaagtgcgg tcctgctggc attctggagg
      481 gaaatacaac cgacagatgt atgtaaggcc aacgtgctca aatcttcata cagaaagatt
      541 tgaagtaata ttttaaccgc tagatgaaga gcaagcgcat ggagcgacaa aatgaataaa
      601 gaacaatctg ctgatgatcc ctccgtggat ctgattcgtg taaaaaatat gcttaattgc
      661 accatttcta tgagttaccc tgatgttgta attgcatgta tagaacataa ggtgtctctg
      721 gaagcattca gggcaattga ggcagcgttg gtgaagcacg ataataatat gaaggattat
      781 tccctggtgg ttgactgatc accataactg ctaatcattc aaactactta acctgtgaca
      841 gagccaacac gcagtctgtc actgtcagga aagtggtaaa actgcaactc aattactgca
      901 atgccctcgt aattaagtga atttacaata tcgtcctgtt cggagggaag aacgcgggat
      961 gttcattctt catcactttt aattgatgta tatgctatct tttctgacgt tagcctccga
     1021 cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgatc aagagcgatg
     1081 ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt tctgcgggtt
     1141 ctgttcttcg ttgacatgag gttgccccgt attcagtgtc gctgatttgt attgtctgaa
     1201 gttgttttta cgttaagttg atgcggatca attaatacga tacctgcgtc ataattga
//
LOCUS       DAZFWP020000403         1251 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFWP020000403 DAZFWP020000000
VERSION     DAZFWP020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.412"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1187
                     /locus_tag="ACHK0Z_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /pseudo
     CDS             1..1187
                     /locus_tag="ACHK0Z_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            1184..>1251
                     /locus_tag="ACHK0Z_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             1184..>1251
                     /locus_tag="ACHK0Z_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HGU4505144.1"
                     /translation="MTALLTLEEIKAHLRVDHDADD"
BASE COUNT          291 a          292 c          415 g          253 t
ORIGIN      
        1 ccgttcgcgt cggccgggaa aacagtttca gcacaactgc ctgacggcgc atctgacggt
       61 aataaggcaa tcagtgaaac agagcatgat gcagtgaaag cgatgttcag ggggatactg
      121 agaaaatgac ggaacgggaa ctgtccatta ttcgtgcact gggcgaagaa ttctccacgg
      181 tgctggcgga tttacagcgc acatttgagg ggaagatggc cgcgcaggca caagcgtttg
      241 aagagaaact ggcttccctg tcggcggtat tacagaagca tgtgacggtg gatgaggtac
      301 atccggttct gcaggcgatg gtggatgacg ctgtggggac cattccggta ccgcgtgatg
      361 gtcgtgatta tgatccggat gtactgcagc aggcggtgaa tgatgcggtt gcgaatattc
      421 cggtaccggc ggacggcaaa agtatcaccc ccgatgatgt gcgtccgatg cttgagcaga
      481 tggtgaaaga ggctgtaagc catatccctg ctccgcgtga cggtcgtgat tatgatccgg
      541 aagtactgaa gcaggcggtg aatgatgcgg ttgcgaatat tccggtaccg gcggacggca
      601 aaagtatcac ccccgatgat gtgcgtccga tgcttgagca gatggtgaaa gaggccgtaa
      661 gccatatccc tgctccgcgt gatggtcgtg attacgatcc ggaagtactg aagcaggcgg
      721 ttctggaggc ggtgaatgcc cttccggctc cgcaggacgg gcgtgatgcc acggcactgg
      781 aagtgctccc cgctattgac gatcaaaaat cctttccccg gggcacgtat gccacacacc
      841 tgggtggact ctggcgggcg tatgaaaaaa cgcacgggat gcggggatgg gaatgcctgg
      901 ttgacggggt ggcggatatc gacgtcagca tgacggatga gcggttgttc tctgtggtga
      961 tccggcagag cagtggccag tgtacggaaa aaacattttc cctgccggtg atgctctacc
     1021 gcggtgtgtt cagagccggt gaaacctacc accccggcga tacggtgacg tgggggggct
     1081 cgctgtggca ctgcaacagt atgaccggtg ataaacccgg agaagctcat tcatcaggct
     1141 ggaccctggc tgcaaaacgt ggacgggatg caggaggcgg aaaatgacgg cattactgac
     1201 actggaagag atcaaggcac atctgcgtgt tgaccatgac gcggatgatg a
//
LOCUS       DAZFWP020000404         1249 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFWP020000404 DAZFWP020000000
VERSION     DAZFWP020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.413"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(561..1133)
                     /gene="hdeD"
                     /locus_tag="ACHK0Z_RS26705"
                     /old_locus_tag="pgaptmp_005341"
     CDS             complement(561..1133)
                     /gene="hdeD"
                     /locus_tag="ACHK0Z_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU4505145.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          424 a          277 c          240 g          308 t
ORIGIN      
        1 gcggattact gcccaagaat aagttatcaa taaccagttt gtgatctctg aagaatatta
       61 ctaaagttaa aatgtaatcc gatttaaata tcgagtctcc ttgtttcgac ttaagctggc
      121 aattggattg ccagctttct tttaacgcat tgaaattacc tgagtaaata acgcattcat
      181 gtcttcaggt ttttatatag cgtctggaga cacggatatt tatgcaatgt aatcaacgcc
      241 agttgcaaaa aaatgaaccc ggattactta tttaaccgcc catagcatca acgcttacgg
      301 aactctttgt tcactttccc acagcacttt taaaacatta aacctgacta cggaaaatat
      361 cagccatgat aatgtttgaa atggctaatt tgccatggag tgaaaaaaat tagatgaaat
      421 tcagtaggtt gaaatgatca ctagcaggta attatttcaa tgatagtgcg caattgatct
      481 acaacactgc gtagcggaga gagtattaat cggatcatag tcacatcaag tgactatgat
      541 ccgggtgaca accgggataa ttattgctgc ttaacgaaca aactggcgaa gctgaacagg
      601 ctggcggcgc taaatatcag ttcaattccc accagtgtgg aaaccagcgt tacagacacc
      661 atcggcgttg caccaaggaa tatccaggca atgacgatat ccagcacacc aataacgagc
      721 tgtagccagc tgcctttcat tgaacgctga cgataccaac tcatcaggcg aataacccct
      781 gcaacacaga acaaaccggc aataaatgcc gcaatggcaa aaatgcccag ctccggtgcg
      841 cggatgaaga aatagccgat caataaatag gcgactgcga cgaggaaacc ggataatacc
      901 ggccagaaat tatgactgcg gttgctgaat aacccgacaa taagcgcaat acccgagcag
      961 attaataatg cacccactac tgtgcttaaa atatcgccag agacgaacgg gaaactgata
     1021 cacagcaacc cgacgataaa cagcagcacg gcaataaact ggattgctct gcgatgcttt
     1081 ttcagcatct ccagatcaaa cttcaaaatt gttgccttat ctatatataa catagaacca
     1141 ccctataaaa ttaagaagaa aatcccctgc tatcaatcta tgccaaaaac gcgtctaaga
     1201 atgcagtcga tttaataaaa atttcctaat tgcagtatct gatgcatct
//
LOCUS       DAZFWP020000405         1247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFWP020000405 DAZFWP020000000
VERSION     DAZFWP020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.414"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1247
                     /locus_tag="ACHK0Z_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     rRNA            <1..1247
                     /locus_tag="ACHK0Z_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          311 a          291 c          391 g          254 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacaga actttccaga
      721 gatggattgg tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca cctcctt
//
LOCUS       DAZFWP020000406         1245 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFWP020000406 DAZFWP020000000
VERSION     DAZFWP020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.415"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            56..1153
                     /locus_tag="ACHK0Z_RS26715"
                     /old_locus_tag="pgaptmp_005343"
     CDS             56..1153
                     /locus_tag="ACHK0Z_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4505146.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          352 a          272 c          278 g          343 t
ORIGIN      
        1 atttatcgcg cagtgacatc attttttaat aatagttcaa aaaaaagggc tcacgatgaa
       61 aaaattaaca gtggcaattt ctgctgtagc tgcatcagta ctgatggcga tgtctgctca
      121 ggcagctgaa atttataata aagacagtaa caagctggat ctgtacggga aagttaatgc
      181 caagcactac ttttcctcta acgatgcaga tgatggtgat actacttatg ttcgtctggg
      241 cttcaaaggc gaaacccaaa tcaacgatca gctgactggt ttcggtcagt gggaatatga
      301 attcaaaggc aaccgcgctg aatctcaagg ttcctccaaa gacaaaaccc gtcttgcatt
      361 tgcaggcctg aaattcggtg actacggctc aatcgattac ggccgtaact acggtgtagc
      421 atacgacatc ggtgcgtgga ctgacgttct gccagaattc ggtggcgata cctggaccca
      481 aacagatgtg ttcatgactg gtcgcaccac tggtgttgca acctatcgta acaacgactt
      541 ctttggtctg gttgatggtc tgaactttgc tgctcagtac caaggcaaaa acgatcgtag
      601 cgatttcgat aactacaccg aaggtaacgg tgatggcttc ggtttctctg ctacctatga
      661 atacgaagga ttcggtatcg gtgcaactta tgcgaaatct gatcgtaccg acactcaagt
      721 taatgcaggg aaagttcttc ctgaagtatt tgcttccggt aaaaatgcag aagtttgggc
      781 cgcaggtctg aaatatgacg ctaacaacat ttacctggcc actacctatt ctgaaaccca
      841 gaatatgact gtatttgctg atcacttcgt tgctaataaa gctcaaaact tcgaagctgt
      901 tgcacaatat cagttcgatt tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg
      961 taaggatctt ggagtatggg gcgatcagga cttagtcaaa tatgttgatg taggtgcaac
     1021 ctattacttc aacaaaaata tgtctacttt cgttgattac aaaatcaacc tgcttgacaa
     1081 aaatgacttc actaaagcac tcggtgtaag cactgatgac atcgttgctg taggtctggt
     1141 ttaccagttc taatctgatt acgaaaaaga tatgttgcgg gaggcgttgc ctccccaaca
     1201 tataagtggc tccctcaagc cacttccttt aggagcacaa ccttg
//
LOCUS       DAZFWP020000407         1229 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFWP020000407 DAZFWP020000000
VERSION     DAZFWP020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.416"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..482
                     /locus_tag="ACHK0Z_RS26720"
                     /old_locus_tag="pgaptmp_005344"
     CDS             <1..482
                     /locus_tag="ACHK0Z_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HGU4505147.1"
                     /translation="QYLINENWQFVPHSNTIFFDGYIAPGWTDYPVTELTVGDSLRLY
                     HVDDSKERECWLVINNPLATVKLCDNNLFITDVQAADLLCAISNEAVMDRIYCVIGTI
                     HVDIHMLRNAKKDDIIDSTGYHLFGGCRLIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            513..1028
                     /locus_tag="ACHK0Z_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     CDS             513..1028
                     /locus_tag="ACHK0Z_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HGU4505148.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRCVQSTENLDYSNA
                     MISNSFSNSVV"
BASE COUNT          363 a          195 c          259 g          412 t
ORIGIN      
        1 tacaatatct aattaatgaa aattggcaat ttgttccaca ttctaatacc attttctttg
       61 atgggtatat tgctcctggg tggactgatt acccggttac agagctgact gttggtgata
      121 gtttaaggct ctatcatgtt gatgacagca aagaaagaga gtgttggctt gttattaata
      181 accctttagc aaccgtaaaa ctttgtgata ataatttatt tattacggat gtccaggctg
      241 cagatttgtt atgtgctatc agcaatgagg ctgtaatgga tcgcatttat tgtgtgattg
      301 gaacaattca tgttgatata catatgttgc gcaatgccaa aaaagatgat attatcgatt
      361 caactggata tcatcttttt ggtgggtgcc ggttaatacg caataatacg actatagcat
      421 acgggagcat tgtaaaaatc aacgaagatt tttattttac tgttagttta gtatgtgatt
      481 aatcaggaat aagttatata gggagatgta tcatgtcgtc atcgttatca ggaataatac
      541 tcaatacttc gctgacaagc caccatatgt catggggcaa gtttagcaga actcttcatg
      601 tattgaatga agatatcgta gatgaaattc agcttaaagc ccgaacacaa caacgccacc
      661 atcgagtata ttcagaaatg gcggattact ccgttgtctc ctttcggggc aaagaatatt
      721 ctgttcgttt tattaaagat tacaaaacag ataattatgt gtacaaagta caaaagatga
      781 ctcaggaaag tgggtgtctc tcaagattat ttagctttct ttctggcggt gtgactaagg
      841 ctttggaaag taaactaaat gagaggcata tcactccact ttctccaacc tggtttcctc
      901 gaactccact ggagggggtc ttaacggaaa gaggattatc ttctttatta cgttgcgtac
      961 agtctactga aaaccttgat tatagtaatg ctatgatatc aaactctttc tctaactctg
     1021 tagtgtgatt atcatttgag ctttcctcct ggtttaactc cagaataatt gtgattctgg
     1081 agtttttctg tttgttttat gtcgataata cattgttttt aggaaaatat gggatgaata
     1141 ttcagagtga gtttggctgg cagaagtcag agattctgtg atgaggaggg gattgagctg
     1201 aatggatgaa ttccttccag ctcataatt
//
LOCUS       DAZFWP020000408         1227 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFWP020000408 DAZFWP020000000
VERSION     DAZFWP020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.417"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            171..347
                     /locus_tag="ACHK0Z_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             171..347
                     /locus_tag="ACHK0Z_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505149.1"
                     /translation="MADFGSTKYNVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            344..859
                     /locus_tag="ACHK0Z_RS26735"
                     /old_locus_tag="pgaptmp_005347"
     CDS             344..859
                     /locus_tag="ACHK0Z_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505150.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKDVTDFQGKQWAGDWKY
                     VPTKEDCNDRPNYEIQALFIGPPVPVTSEGLVKAVRFYEQVKRENPPVETGAWKDAVD
                     WVLREACCAAILGKADNPPASGNQVSELAMWVKRLVSQLKKAKPDCKLPDKAMDYLKR
                     NGLINEENILR"
     gene            1150..>1227
                     /locus_tag="ACHK0Z_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             1150..>1227
                     /locus_tag="ACHK0Z_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4505151.1"
                     /translation="MKQQKAMLIALIVICITVVVTVLVTR"
BASE COUNT          376 a          232 c          351 g          268 t
ORIGIN      
        1 aaagttcaaa ataattattg aaacgggaat cgccggtgga gattccgagg atgaattcga
       61 agtgaatgat gatgcgacgc ctgatgaaat tcataacgaa gcaaaagaaa ttttctttaa
      121 ctactgcaat tactcatatc acgaaataaa agacgaagag gaagaacaaa atggctgatt
      181 ttggttcaac taaatacaac gtcagttttg aagaatggca tgaactgtta atggactatg
      241 cagagttacg tggtggaagt gccgccgatg ctgaagcatg gcgtgatgac tatgaagcag
      301 gaaaaactcc ggtcgaagca tattgtgatg agtggggcga tgaatgagcg agattaatta
      361 tcaggaaggg catgaaacgg cagggcaggc aaaaccagtg gcatggcgat atcgctacgt
      421 gaaaaaagac gttacagact ttcaggggaa gcagtgggct ggtgactgga aatatgtacc
      481 gacaaaagag gattgtaacg acaggccgaa ctatgaaatt caggccttat tcatcggccc
      541 gccagtcccg gtgacatcag aaggactggt taaagctgtg cgcttttatg aacaggtaaa
      601 gcgtgagaat ccgccagtcg aaacaggagc atggaaggat gctgttgact gggtgctcag
      661 agaggcctgc tgtgctgcca ttctgggtaa agccgacaat ccaccagcat ccggcaatca
      721 ggttagcgaa ttagcaatgt gggttaaacg gctggtcagc caactgaaaa aagcgaagcc
      781 ggactgcaaa ttaccggata aggcgatgga ctacctgaag cgaaacggac tgataaacga
      841 ggagaatatt ttacgatgac ctggcctgaa gcgttcataa cggtaggaat tgcaatggcg
      901 gtggcgctgg tggtgtattc gatttgccgc tggggataaa aacggtttgc ggggaaagaa
      961 gagttaagta gaattgctgc gggtgcttga ggctgtctgc ctcgggcatg ccgacgtaag
     1021 gcagacagag aaaagcccca gttaacatta tgcgtcttgc aggacgctta acattaatct
     1081 gaggccatat ctatgacttg cacacttaga ttagcctctt atgcgccgaa aggcaaggag
     1141 cagcaggcta tgaagcagca aaaggcgatg ctaatcgccc tgatcgtcat ctgtattacc
     1201 gtcgttgtaa cggtactggt aacgagg
//
LOCUS       DAZFWP020000409         1225 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFWP020000409 DAZFWP020000000
VERSION     DAZFWP020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.418"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(100..>1225)
                     /locus_tag="ACHK0Z_RS26745"
                     /old_locus_tag="pgaptmp_005349"
     CDS             complement(100..>1225)
                     /locus_tag="ACHK0Z_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HGU4505152.1"
                     /translation="FTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSN
                     YTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNS
                     MKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLEKYYQSYLANADIEIV
                     PNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKN
                     LKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVI
                     PSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILK
                     TLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          383 a          246 c          207 g          389 t
ORIGIN      
        1 acaaattgaa ccaaatttat taggcgttta cgccggctta ttattagcga tgaaaaacaa
       61 gtaaaaaata aaaaaggctg cataatgcgg cctttttatt tattcaaacc aattatgaat
      121 aacctcttcg aatctttgag ttacgccatc ccaactgtag tgttcaaaca caaagtcctg
      181 accttgcttt gcaactgctg ttaattcagg attcgctaac gtttttaaaa tatcgctgct
      241 tatggagtct gcagtcatag gttcttttaa atggaatccc gtagtatttt ctttgacaaa
      301 ttccgtcatc cccccacgcg tactcactaa aacaggctta ccagcaccca tagcttcaat
      361 tgccaccatg caaaatggtt cctggaactg ggaaggaatg acgactaaat ctgccagtgg
      421 gtaataacaa tgcatctttt caggcggtat gcttcctaac ataatgcaac gatccttcaa
      481 acgttttgca atttccctga cattgcgttg atatgcacct ttgtcacttt tactcatttc
      541 tgtataatca ccaatcacaa ctaattttaa atttttgtga gcagcggcca atttttcgaa
      601 tgattgcatc aacagcaata tgcctttgtc agggacgata cgcccagcat aaaaaataat
      661 tttctcttca ggggagatat taagttcgct tctagtgatc ggctgaaaat tagattgata
      721 ggtttctaaa tcaattccat ttggtacaat ctcaatatct gcatttgcta aataagattg
      781 gtaatacttt tccaagtata aactaggcac tatcatttta acattttgct caagcaattt
      841 tggttcaaat gcattatgca tatgtataac cactcttgcc tgaggagctc gcttacgaat
      901 ttgagtgtat agcttcatgc tattatgaac tacaataaca ctttctttcg ttataggaaa
      961 atcatgtgca atattcaata ttctctgtga atatggaaga ggatcaagtc ttgtccattt
     1021 ttgaaataac cgcttatata tgcgactgaa tccaacccta tgaatactac aattatcatt
     1081 aacaaaggtg taatttgagt atcctggatt tttgatacaa acaatacgat ttggaaaatt
     1141 ggtacgctga gcaacctgat acatccacgt ttcaacagca gccgctccgc gaggaggaat
     1201 tgaaaaaata ggagtaaccg taaat
//
LOCUS       DAZFWP020000410         1215 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFWP020000410 DAZFWP020000000
VERSION     DAZFWP020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.419"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(49..936)
                     /gene="rhtA"
                     /locus_tag="ACHK0Z_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             complement(49..936)
                     /gene="rhtA"
                     /locus_tag="ACHK0Z_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU4505153.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          362 a          308 c          292 g          253 t
ORIGIN      
        1 tagcgagaaa ttctgcatgg ttatgcataa ccatgcagaa atgtaaattt aattaatgtc
       61 taattctttt attttgctct ctttgcgtac tgtcagcgta gaccccattg aagcggcgat
      121 gatagcgccg agcgccagca gctgtatggg tgtcagtgtt tctccgagga aaatcatccc
      181 ggaaacggca gccagcgccg gttccatgct catcagcgta ccaaatgtcc gtgttggcaa
      241 acgggtgagg gcaatcattt ccagcgaata aggcagagcg gtcgagagaa tagcgacagc
      301 cagacccaat ggaataaccg accagtgcca gagtgcttca ccagcctgaa gcgctccaat
      361 tggcacgaaa attaacgctg caatcaacga accaattgcc accgtcgcag ggccatgttc
      421 cgctcctgcg cgttgcccac ttaaaatgta aatagcccaa caagccccgg cccccagtgc
      481 cagcgcacag ccggttaaat cgacatggga aacgtcttgc cccagcggta gcaggaacca
      541 cagaccaaga accgccagca caacccagac gaaatctacc gggcgacgag aagagaacag
      601 cgccaccgcc agtggtccgg tgaactccag cgccaccgca atacccagcg gtactgtctg
      661 aatagaaaga taaaaaagat aattcatccc acccagcgaa acgccgtaaa acaacagcgg
      721 taaccgttgc tctttggcaa agcgcagtcg ccatggctta aagaacgcga tgaggatcag
      781 cgttcctaat gccagacgca gcgcagtgac acccggtgcg cccaccagag gaaaaagtga
      841 cttagctaac gaggctccac cctgaataga cgccatggca acgagcaata tgattattgg
      901 taaccagacc ggcattttac gtaatgaacc aggcatcctt tctcccacaa atatctagac
      961 ttaagtaaag cgtggagtgt actggatata cccaatgctg gttgagcatt tgttgaaaaa
     1021 attttccccc gttttgacta aaatgcgcca ggattgatgg aatcattagt ctggtgatta
     1081 ggaataatct ggatgaatga cagggaaaac atgcgtaata cttacgcagt tctctgaaaa
     1141 agtgatttaa atttagatgg atagcggtgt atggaaacgt tctgttacat gaaatggccc
     1201 gttagacatc acaaa
//
LOCUS       DAZFWP020000411         1196 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFWP020000411 DAZFWP020000000
VERSION     DAZFWP020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.420"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..179)
                     /locus_tag="ACHK0Z_RS26755"
                     /old_locus_tag="pgaptmp_005351"
     CDS             complement(<1..179)
                     /locus_tag="ACHK0Z_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505154.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRITFNDGEVDQRAL"
     gene            complement(163..393)
                     /gene="dicC"
                     /locus_tag="ACHK0Z_RS26760"
                     /old_locus_tag="pgaptmp_005352"
     CDS             complement(163..393)
                     /gene="dicC"
                     /locus_tag="ACHK0Z_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HGU4505155.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            477..884
                     /gene="dicA"
                     /locus_tag="ACHK0Z_RS26765"
                     /old_locus_tag="pgaptmp_005353"
     CDS             477..884
                     /gene="dicA"
                     /locus_tag="ACHK0Z_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4505156.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKVLKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            1051..>1196
                     /locus_tag="ACHK0Z_RS26770"
                     /old_locus_tag="pgaptmp_005354"
     CDS             1051..>1196
                     /locus_tag="ACHK0Z_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4505157.1"
                     /translation="MDTLNLGNNESLVCGVFSNLDGTFTAMTYTRSKTFKTEAGARRW
                     LARNT"
BASE COUNT          351 a          297 c          248 g          300 t
ORIGIN      
        1 agtgcccgtt gatcaacctc gccatcatta aacgtgatgc gttgaacatc gatgttcggg
       61 cgttctttca gagcccagaa tgcttccgtg attaatatcg tcgcctgttc ctgtgtcatt
      121 cctggtcggc acacccaggc atccagagcc tcacaaacct gttcaggggt gattttcatt
      181 gttcaaccgc cccgcccgct ttgccttacg atattcgtca taaactttgg ggtcgtactg
      241 aagttccccg ccggatgcct cttgcaggcg catcgcgcga ccttcaggta ctagctcccc
      301 tttccagcta taaagcgaag ccaaacgaat accagcagct tgtgcaagtt ttgtttttga
      361 accgaaatac aaaagtgcgt cagttttaag catttaaagc accttgattg ttagccatga
      421 ctaacaaaat agatgttaac aaaaacatag tcaataagat ttagcattag ctaactatgg
      481 atacaaaaaa tttaaccatt ggcgaacgca ttagatatcg ccgaaaaaac ctcaaataca
      541 cccaaaggtc tcttgctaaa gtcctgaaaa tttctcacgt atcggtttca caatgggagc
      601 gggatgatag cgaacctaca ggaaagaatc tttttgccct cagcaaagta ctgcagtgct
      661 cgccaacatg gattctattt ggcgatgaag acaagcagcc atcaccacct attgaggagc
      721 cagttgctct atcccccaaa gaacatgagc tccttgagct ttttaatgca ttgcctgaat
      781 cagaacagga tgctcagctc accgagatgc gcgcccgagt aaaaaatttc aacaaactct
      841 ttgaagagtt attaaaagcc cgtcaacgaa caaacaaaag ataacgcaat caatgcgtta
      901 tctttttggt tgcccaaaat gttagtcatg gcaaacaaaa atacttgacc aaattgttag
      961 tcatggctaa tcttgcttgc atcaagacac cgcacggtgt tctcagcaaa cagttccgct
     1021 accccggcgt taaggggaaa tgaggtcaac atggatacgc tcaatcttgg caacaacgaa
     1081 tctctggtat gcggagtatt ctccaacctg gacggcacgt ttaccgcgat gacgtatacc
     1141 agaagcaaaa cgtttaaaac tgaagctggc gcgcgtcgct ggttagccag aaacac
//
LOCUS       DAZFWP020000412         1194 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFWP020000412 DAZFWP020000000
VERSION     DAZFWP020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.421"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1194
                     /locus_tag="ACHK0Z_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             <1..>1194
                     /locus_tag="ACHK0Z_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4505158.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPE
                     VSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRY
                     AVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYKGQPVTRLAVMLN
                     LLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGSKMRTPHLVPLSQQA
                     VAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMAC
                     SALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREQC
                     ISPFE"
BASE COUNT          322 a          290 c          289 g          293 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggta catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa agagaagact ctggcacttg
      121 gtgtgtatcc agaagtatct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcgga gggggttgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc tttcgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaagta ctcaaaagcc tcgagacaca cgtattcccc tttatcggca
      361 atcgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aatatctgct gtaatgcgtt
      481 atgccgtaca gtctggcatc atcagatata acccggctct ggatatggct ggtgcattga
      541 ccacggtaaa acgccagcat cgccctgctc tggatctttc acgcctgcct gaacttctgt
      601 cgcgtattga cggttataaa ggccagcctg tcacccgact ggcagttatg ctgaatttac
      661 tggtttttat tcgttccagt gaactcagat atgcccgctg gtctgaaatt gatattgaca
      721 atgccatgtg gactattcca gccgaacgcg aacctctgcc aggcgtaaaa ttttcatacc
      781 ggggctccaa gatgcgaaca ccacatcttg tgccactcag ccaacaggct gtagccatac
      841 tggcagaact tcagacatgg gcaggtgaaa atggtctgat ctttacggga gcacatgacc
      901 cacgtaaacc aatcagtgaa aacaccgtaa ataaagccct gagggttatg gggtatgaca
      961 caacccagga tgtctgtggt catggattcc gggcgatggc atgtagtgca ttaatagaat
     1021 caggtttgtg gtcccgcgat gctgtggaac gccagatgag ccatcaggag cgtaatggtg
     1081 tacgtgctgc gtatatccat aaagccgaac atctggaaga acgtcgactt atgctacagt
     1141 ggtgggcaga ttttctggat gcgaacagag aacaatgtat cagcccgttt gaat
//
LOCUS       DAZFWP020000413         1192 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFWP020000413 DAZFWP020000000
VERSION     DAZFWP020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.422"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..318
                     /locus_tag="ACHK0Z_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             16..318
                     /locus_tag="ACHK0Z_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4505159.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            321..680
                     /locus_tag="ACHK0Z_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             321..680
                     /locus_tag="ACHK0Z_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4505160.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(727..1119)
                     /locus_tag="ACHK0Z_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             complement(727..1119)
                     /locus_tag="ACHK0Z_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4505161.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
BASE COUNT          344 a          243 c          267 g          338 t
ORIGIN      
        1 gactactata cagttatgaa gattatctca gttaaaacac tcagggattt ttgggcggag
       61 aatcctgatg cagaacaacc gctaagggca tgggtggatg aggcggcaaa agctgactgg
      121 aaaagcccag cagacattaa ggcacaatac cgaacggcca gtatattgaa aaaccggcgc
      181 gtggtattca acataaaagg taatcattac cgtttaattg ttgcaattgc gtatcagcga
      241 ggatgggtat ttgttaaatt catcggaaac cacaaggaat acgatgctat tgatgctgat
      301 accatcgaac tggagtaagc atgaacatca aacctattcg tacagagcaa gattatgaag
      361 ccgcgttgcg tgctgttgaa cccatgttcg acaatgagcc cgaaattgac acgcctgagg
      421 gggatttttt tgaggtgatg tgtttgctaa tagaggaata tgaaaaaaaa cattatccca
      481 ttgagccacc atccccaatt gaagctataa gatttcgcat ggaacagcag gggctgactg
      541 tgaaagattt ggaacccgca attgggaaaa aaaatcgggt ttatgaggtg ttgaatggta
      601 ccagaagcct tacgttacca atgattcgcc gtcttcataa tcaatttggt atccccctgg
      661 aaagcctggt tggattataa aatctgctag tcatttgcct gatgctcgtt ccagaaaagg
      721 aatgcatcag gcagttttgt ttttctgccg cagtaactct tcaagtttcc gtttatagaa
      781 atcgcgtttt tgctccatat cacgaatgat ctgctctgcg tcgctttgag gtaactcatc
      841 taaaagcgat atgatttttc gttgttgttc tgtaagttgc ggttggttgt cattactgga
      901 tacagccatt ttatcgccga gagtttcttc ttccataaag aaccaatgga cgggatgttg
      961 tgagagctct gctaattttt ccagtttatc cattcttggc atcacgcctt tcaaccaacc
     1021 ttgcacggat tggggtttta caccaagacg tcttcccagc tctgactggt ttatattcaa
     1081 ttcctgcaac acctgctgaa ggcgttttac aaagatcatc accacccctc gtaaactagt
     1141 tccgcgatcc tacagaaaaa attgataagt ggcattacaa atagaagttg aa
//
LOCUS       DAZFWP020000414         1186 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFWP020000414 DAZFWP020000000
VERSION     DAZFWP020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.423"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            647..1147
                     /locus_tag="ACHK0Z_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /pseudo
     CDS             647..1147
                     /locus_tag="ACHK0Z_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          340 a          226 c          252 g          368 t
ORIGIN      
        1 catgccagtt aacagctatc cgtatggcgt gttcggtttt gcgaataaaa aatggttact
       61 gtagaagcgg atttccacca catgtcaacc atgaatggtc agtgatgcga gacgactggg
      121 atgatttggt cgatgacttc ttccggcgaa tactgataga aacattgaaa tattgtaaga
      181 gaaagcgttc aaaattctgt ggcacgtgaa aaattctaca gcgtcatcac cttattcagt
      241 cgaccttcaa aataaacgtt taacagagac aatcaccatt tggattgtcc gctgaggagg
      301 cgaacaattt cctagacagc tttatcatgt cagcccacat tgttggcgct ggcaatacta
      361 aggacaaacg atggggaagt agctttttag cgttattttc gggagtaaaa aacgtggtat
      421 ctttccgtat gtcagttgta tcttgctggt attcgtagtc gtgataaggc atacaacgat
      481 atttatcttg acgttagtga agaataagtt gttttgtgat ctatgttaat ctgggattac
      541 aatccgggct tgatacatcg catcacgact tttattctct tcagaataga atatgttact
      601 ccccgcttta caggctcagg atttgaatta caaattatac ttttaagtga acaataacca
      661 agggtatttc ttatttcctg aaaccaggct tggcccattt cgggatgagt ttgaaaaaac
      721 tcttgacgca tacgatagtg atgaaataaa cgaaacaaat tacgtcaaca aactcaggcg
      781 tcttgcctga catgctcctg acatcattga tattcacgct catctggcat atgcatttgc
      841 gctctttatg ctgcaattct ggcgaatgtt catttacagc gtcatcagga tgccgttata
      901 ctcattgata aaatactaac atataaccct gagaataatc agggaacacg ctggttactg
      961 ggggcggaat tgttacgtgc tggcaagcat ggcagggctc tgtacgtatt gacaggacat
     1021 gtcaatgaat ttccccccta ctggtacgag cttgctcttt tacattttat gaatgaggca
     1081 tatattaatg ctgcaacggc atttcgatat ggatttttaa caagtatata ttgttgaaat
     1141 gctttgttag aatctttatc cattccctag ggctgtatag tatgat
//
LOCUS       DAZFWP020000415         1184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFWP020000415 DAZFWP020000000
VERSION     DAZFWP020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.424"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..367
                     /gene="ompN"
                     /locus_tag="ACHK0Z_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /pseudo
     CDS             1..367
                     /gene="ompN"
                     /locus_tag="ACHK0Z_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            complement(617..1147)
                     /locus_tag="ACHK0Z_RS26805"
                     /old_locus_tag="pgaptmp_005361"
     CDS             complement(617..1147)
                     /locus_tag="ACHK0Z_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505162.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
BASE COUNT          326 a          264 c          237 g          357 t
ORIGIN      
        1 acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag tttataataa
       61 agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt tttctgataa
      121 ttcggcgaaa gatggcgacc agagctatgc gcgtctgggt tttaaaggcg aaacccaaat
      181 taacgatcaa ctcactggct acggtcaatg ggaatacaat attcaggcaa acaacactga
      241 atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc gggctgaaat ttgcagatta
      301 cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag gctggaccga
      361 tatgcttgta accaggtgaa tcatttgata ccaacgaggg ttttagtgaa aggaggtgaa
      421 atcacctgta agatactgaa ataaaacagg tgatgtctgg atgagcgctt aaagtctatt
      481 tgataccgat tgtgcaataa ttaatcgttt attctaaaat cggcaaaact tttgatactg
      541 cgatctcaac tttttaaagc gtttaaaact tcattaaatc tatatggcaa ctaacatacg
      601 ggccagcctc gccaaactaa cccgctttag attctaccgg atttgattgc ctacttgtct
      661 cggttttttc caaaatctct ctgacgcaat cgtctggcat atctacaatc atcaacgcag
      721 ccaggcgctt cctgccttct agttgtaata aatcctggtt ttctttatcc agcagtgtca
      781 gaaccgtctc tacacctttg cgagcaagga gattggttaa ctgttctcgt tcgtctttcg
      841 taaggaatga aagcatctgt gtaagttgat ctcccttatc accagaacaa ccaaaattac
      901 cacaaatacc aacatcgttt ggtgattcac ccttgccctc tttaagccac tcaagactaa
      961 ctccttcact ttcagaaatt cgtataagga tactttctct gggggctggt tgtgtttgtc
     1021 ctcccttgaa atagctgttt aatgtgttga tattgattcc ccacgctctg gcagctgcac
     1081 ttcttgaagg gtagcgcttt gtaagcattc caatacgttc aataacaggt tcttttccct
     1141 gcgacataaa gatacctctt ggtgcttttc taaggaaagg tttc
//
LOCUS       DAZFWP020000416         1177 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFWP020000416 DAZFWP020000000
VERSION     DAZFWP020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.425"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..174
                     /locus_tag="ACHK0Z_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             <1..174
                     /locus_tag="ACHK0Z_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505163.1"
                     /translation="YCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFT
                     GICNDLAAASTFV"
     gene            138..713
                     /locus_tag="ACHK0Z_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             138..713
                     /locus_tag="ACHK0Z_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4505164.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPVFRQKLSDGWYQVRIAG
                     RDAGETELSSRLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIATAIWMPGISIA
                     FSDILFSMGAAMTLGGVAQMLAPKASTATTTSTDNGKQNTYFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVISQEISTADEGDGGEIVVIGR"
     gene            774..>1177
                     /locus_tag="ACHK0Z_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             774..>1177
                     /locus_tag="ACHK0Z_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4505165.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEENTNISGVTVVFRSGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEV"
BASE COUNT          271 a          285 c          380 g          241 t
ORIGIN      
        1 tactgtggtg acggcgagct gctgcaccat attcctgaac aactgagcaa acgagagagg
       61 tataccgaca aatggcagcg acgcacacac tccctctggc gtcaccgggc atggcgcgca
      121 tctgccttta cggggatttg caacgatttg gccgccgcat cgaccttcgt gtgaaaacgg
      181 gggctgaagc catccgcgca ctggccacac agctcccggt gtttcgtcag aaactgagcg
      241 acggctggta tcaggtacgg attgccgggc gtgatgcagg cgaaaccgaa ttatcatccc
      301 gtcttaatga gccgctggca aatggtgcag tgatccacat cgtgccgcgt cttgcgggag
      361 caaaaagtgg cggtgtgttt caggcggtgc tgggtgcggc gttgattgct acggcaatct
      421 ggatgccggg gatcagtatc gctttcagtg acattctctt ttcaatgggt gcggcgatga
      481 cgcttggcgg tgttgcacag atgctggcac cgaaagcgag taccgcaaca acgaccagca
      541 cggataacgg taagcagaac acgtatttct catcactgga taacatggtt gcccagggca
      601 atgttctgcc tgttctgtac ggtgaaatgc gtgtggggtc acgggtaatt tcgcaggaaa
      661 tcagcacggc agacgaaggg gatggtggtg aaatcgtggt gattggtcgc tgatgcagaa
      721 tgttttatgt gaaaccgcct ccgggcggtt ttgtcgttta tggagcgtga ggaatgggta
      781 aaggcagcag taaggggcat accccgcgcg aagcgaagga caacctgaag tccacgcagc
      841 tgctgagtgt gatcgatgcc atcagcgaag ggccgataga aggtccggtg gatggattaa
      901 aaagcgtgct gctgaacagt acgccggtgc tggacagtga ggagaatacc aatatctccg
      961 gcgtcacggt ggtgttccgg tcaggtgagc aggagcagac accgccggag gggtttgaat
     1021 cctccggctc cgagacggtg ctgggtacgg aagtgaaata tgacacgccg ataacccgga
     1081 ccatcacgtc ggcaaacatt gaccgtctgc gcctgacctt cggtgtgcag gcactggtgg
     1141 aaaccacctc aaagggggac aggaatccgt cggaagt
//
LOCUS       DAZFWP020000417         1168 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFWP020000417 DAZFWP020000000
VERSION     DAZFWP020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.426"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..641)
                     /locus_tag="ACHK0Z_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             complement(<1..641)
                     /locus_tag="ACHK0Z_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4505166.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIART"
     gene            complement(1048..1167)
                     /locus_tag="ACHK0Z_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             complement(1048..1167)
                     /locus_tag="ACHK0Z_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4505167.1"
                     /translation="MVMVDFTSRIMSVLADGVLVCGIVVLLWPVIKRNSLHNA"
BASE COUNT          285 a          350 c          269 g          264 t
ORIGIN      
        1 gtgcgcgcaa tcaggtcctg atataacggt ttacccacac cccagcgcgc cgaatcctgg
       61 ctggcccccg tgtccgcact gaatgtcccc tccgcgccct gggtaaatgc cgaaccacca
      121 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacgggag cagttttttg
      181 gcaatatgta acccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga
      241 tgattcagcg tactcatatc ctgcacatca tgcaggcagt ggtcggccgg aataatatcg
      301 ttatatctgc aggcagcccc acccggcgtc actgtactgc ggcgcgccag ctgtttaatg
      361 cgcggatccg gagcatcgta tgaatccggc agcggaagcc cttcaccgta agccatggca
      421 ttggactgcc cggccagtac gatgacgtag taccactccg gctcagttgc accactgacc
      481 accacatcac cttctgctgt aatcgcctgc atcagggtat aaggggttat ggccaccgga
      541 ctaccaaacg gctgccagcc ctctttcagt ttgtgtgtca gcttttccgc aaggtctgac
      601 ggcgacgccg ccctgacaac atcataatgt ttaaatgtca ttattcctcc cggccgggat
      661 agtgtattaa atcagatatg gagtgggctg tagtccggaa gcctgaatga cacacgggga
      721 ctacagccca agaaatgaaa aaaggccacg cagttgcgca gcctgataaa ccctggttaa
      781 aatccacacg ataacaacac aacaatatca gtatctcatg ctattgcccg aacccattcg
      841 ggcatttttt acccataaaa aagcccctcc ggagaggggc atgtttgcat gcacattctt
      901 tttcttgcat ggtgccgggt gcctcccggt gaattcagta tcagcacctg aatccgcgat
      961 tatcacatat acctggttgc tgattgcccc tccgcacagg gggattcacc atgcagtagt
     1021 atttttaata aacagtaaac aaaaaaatca agcattatgc aggctgtttc tttttatcac
     1081 cggccacagc aataccacaa tgccgcagac cagcacccca tccgccagca ccgacatgat
     1141 tctgctggtg aaatccacca tcaccacc
//
LOCUS       DAZFWP020000418         1159 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFWP020000418 DAZFWP020000000
VERSION     DAZFWP020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.427"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            230..1054
                     /gene="gadX"
                     /locus_tag="ACHK0Z_RS26835"
                     /old_locus_tag="pgaptmp_005367"
     CDS             230..1054
                     /gene="gadX"
                     /locus_tag="ACHK0Z_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU4505168.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          336 a          222 c          245 g          356 t
ORIGIN      
        1 agcgattgcc tttaatgtca cgaaaaggga ttatttgctt gctattaatt tcactgcgtt
       61 cttaaacacg tttttgtatt ataaatccag tcatcctgcc cgtagaacat cacacattat
      121 catcctgttc tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg
      181 cgtgctacat taattaacag taatatgttt atgtaatatt aagtcaacta tgcaatcatt
      241 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
      301 tgaatatcgc tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt
      361 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc
      421 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaaatttctt cattactcgt
      481 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
      541 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
      601 ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg
      661 aacacgagtt tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat
      721 cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac
      781 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
      841 acatggcttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
      901 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
      961 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg
     1021 cactgaccgt tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaacc
     1081 cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac
     1141 actccctgtt ggcacggga
//
LOCUS       DAZFWP020000419         1146 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFWP020000419 DAZFWP020000000
VERSION     DAZFWP020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.428"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..561)
                     /locus_tag="ACHK0Z_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /pseudo
     CDS             complement(1..561)
                     /locus_tag="ACHK0Z_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
     gene            819..>1146
                     /locus_tag="ACHK0Z_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             819..>1146
                     /locus_tag="ACHK0Z_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4505169.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTKPVNILLRNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLIHDEID
                     ILRLCVS"
BASE COUNT          394 a          210 c          161 g          381 t
ORIGIN      
        1 taattcctgt ttttgtttac aagaatttta tcgatatgtc tcagctgcgg atcattacta
       61 gcattaacaa tataaaaagc aataaaaatg atagcaaaaa gaaaaaaaaa gcattacata
      121 cttaaacagc ttactgctaa agaaatgttg ttttgtagaa ataatgttat tttcaataat
      181 gcctggcaat gaatactgcc aaacttcatt tttttctttt acagcaaatt tgagacctaa
      241 aacttctatt tgctgattca atgttaacgt tctatcttca ggttcttgtg ctgttaattc
      301 tctgaatgtt actaccagat cagggtcggt agtaattatt atctcaaaac tacctgtaag
      361 ctcgccataa ggaacgacta ttgtgttttc tggtaactca gaaaaagcat cacttcttcc
      421 taacgaaaca tcattaccaa tgataacaag tagacgccca ttaagtattt catactcgca
      481 tccattcaat ggtccgctca acaaacgaat ggcataagat tccaataagt cttgtgataa
      541 gaatttatca ttattttcca tgaataccac ccgctaattc cataagctct agtttagagt
      601 agtgtaacaa gacggaaatt tcatgaccaa attatgatga cacagaataa aaaaattata
      661 atttcgagaa ctattttaaa tgcctctatg cataacttta tttacaattt aaggtataaa
      721 ccctattccc tcaatgttaa gcaaatataa caatcactct tattttaaat tttacacgta
      781 atatttaccc taaatgattt tagagtacag gtgggcatat gcaagtcttt tccagtgatg
      841 tctacttcac tgtaggcact aatgcattat tagcatccca aaaagaatat tacagcgatt
      901 tagttgcgtt ggttgatctt ggacattcgt tcgttgttat tgatgagcat caacatcgaa
      961 atctcaaacc aaatacaaaa cctgtaaaca tccttttgag aaacaatttc atcaggatca
     1021 ataagaatat tacattatct gatttaacgc atttcctggt ttcaaatctt catacacaaa
     1081 atgtgtattc cacacaagaa ccacttatcc atgatgagat agatatcctg cggttatgtg
     1141 tttctt
//
LOCUS       DAZFWP020000420         1128 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFWP020000420 DAZFWP020000000
VERSION     DAZFWP020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.429"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..191
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             3..191
                     /gene="dicB"
                     /locus_tag="ACHK0Z_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4505170.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            188..379
                     /locus_tag="ACHK0Z_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             188..379
                     /locus_tag="ACHK0Z_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4505171.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            472..>1128
                     /locus_tag="ACHK0Z_RS26860"
                     /old_locus_tag="pgaptmp_005372"
     CDS             472..>1128
                     /locus_tag="ACHK0Z_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4505172.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIICEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     R"
BASE COUNT          338 a          240 c          266 g          284 t
ORIGIN      
        1 taatggaaac gttattacca aacgttaata cgtctgaagg ttgttttgaa attggtgtca
       61 ctatcagtaa ccctgtattt actgaagatg ccattaacaa gagaaaacac gaacgggagc
      121 tattaaataa aatatgcatt ctttcaatgc tggcccgttt acgtccgata caaaaaggat
      181 gctggcaatg aatacagcat ttgcacttgt tctgacagtt tttcttgttt ccggagagcc
      241 agttgatatt gcagtcagtg ttcacaggac aatgcaggag tgtgtaactg cagcaaccga
      301 acagaaaatt cccggtaact gttacccggt cgataaagtt attcaccagg ataataacga
      361 aatcccggca ggtctttaaa acagttccgt aataaacatc cgatttcatt cttatatgcc
      421 agcaatggca gggatttgtt cacccttaaa tctgtaatga ggtaaaacaa aatgagtaaa
      481 gtctttattt gcgccgccat tccggacgaa caggcaataa aggaagaagg tgcagtcgct
      541 gtagccacag ctattgaagc tggcgacgaa cgccgtgctc gagcaaaatt tcactggcaa
      601 ttcctagaac attatccggc tgctcaggac tgcgcttata aatttattat ctgcgaggat
      661 aaacctggca taccccgccc tgccctcgat tcatgggatg ctgaatatat gcaggaaaac
      721 cgctgggatg aggagtctgc ttcctttgtt ccggttgaga ctgaatcaga tccgatgaac
      781 gtcacttttg acaagctggc ccctgaagta cagaacgctg tcatggttaa gttcgacaca
      841 tgtgaaaaca tcaccgttga tatggttatt agcgcacagg aattgttgca ggaagacatg
      901 gcaacattcg acggacatat cgttgaagcg ttgatgaaaa tgccagaagt taacgccatg
      961 tatccggagc ttaagctgca tgccatcggg tgggttaagc ataaatgtaa gcctggtgcc
     1021 aaatggcccg aaattcaggc agagatgcgc atctggaaaa aacgtcgcga aggtgaacgc
     1081 aaggaaaccg gaaaatacac gtctgttgtt gatctcgccc gcgccaga
//
LOCUS       DAZFWP020000421         1126 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFWP020000421 DAZFWP020000000
VERSION     DAZFWP020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.430"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..597)
                     /locus_tag="ACHK0Z_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             complement(<1..597)
                     /locus_tag="ACHK0Z_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HGU4505173.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAVASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRTM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNRLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTASQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGI"
     gene            complement(761..871)
                     /locus_tag="ACHK0Z_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /pseudo
     CDS             complement(761..871)
                     /locus_tag="ACHK0Z_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(918..1126)
                     /locus_tag="ACHK0Z_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /pseudo
     CDS             complement(918..1126)
                     /locus_tag="ACHK0Z_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M85 family metallopeptidase"
BASE COUNT          333 a          282 c          193 g          318 t
ORIGIN      
        1 aataccaata atatcgccat tatcgccata tattttttct gcactcccgg caccataata
       61 ttggttgaag caacgaactt cgtttgttac ctcttcattg ctttgagatg cagtaaacat
      121 cttcaggact tttgttgcat cctcagcatc ttcatatact acagcgttac cccctttgcc
      181 aataacatta ccaggcacag gcaacctgtt atagtccaca ctcggtaact ctggcggtgc
      241 ataatcaaca ggaggtaaat caggtctatt cgaatgaaca gcacctcttt ccatagtcct
      301 attcactggc gatgaattca gcatcacctc aattttcctg ctaatctctc ccttaggcca
      361 tcccagcctg tgcaacagat tagtaaagca accactatga ctttctcttg taacgcaaaa
      421 cttattatca gtgacaacaa cacgatatgt tctgttgccc acctttactt gcgccccatt
      481 atccgagcta gcaactgcat cccttacaga ggataaaata cgactgtcag gagaagtcag
      541 gtttctggtt aaagaattcc atgaacaccc caaatttaca gaagatggtg atagcataca
      601 tttcaacctt caaaatgaat caatctttac tttcttaaca aacatcacca tgacatgaca
      661 acaaaaaact ggaaattaac tattgttttt gagtatctgg caactcattc atccttccag
      721 catcccgtca gacgaacgtt tacgaactat aaatcaggta ttactggccc tcgaaatctg
      781 gagatactga cgtatcaaca cggttcagta acacccgata cctcttccag actgtcaaca
      841 acgatgtctc ttcatccgtt gcgatctgca gcatcctgaa tatgaatgcc atagcttacg
      901 gcatcacgaa aagtttatga gcggctcaaa gcatcaagta tagtattact aatcatatcg
      961 accgtatgct gatcaatgag tctgccacga ctgtcatgga cggcgcgtat tacactttcg
     1021 tatacgttgc ttaaatctgc tgcggaaagt ggtattcgct tacctatatc caaaacgtaa
     1081 tccgcatagg cattttcagc acgattagga gcaatgggag cagagt
//
LOCUS       DAZFWP020000422         1114 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFWP020000422 DAZFWP020000000
VERSION     DAZFWP020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.431"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            159..>1114
                     /locus_tag="ACHK0Z_RS26880"
                     /old_locus_tag="pgaptmp_005376"
     CDS             159..>1114
                     /locus_tag="ACHK0Z_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HGU4505174.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFF"
BASE COUNT          388 a          212 c          211 g          303 t
ORIGIN      
        1 acaccgttac attaaaagat aataaaaacc gcaataaaaa aagaataaat caattgtctc
       61 acgcaattat aaaacatcat attgattacg caccttgtat tacaaactca tgtatgtaaa
      121 atacgcgcac cattcaaaaa aaaggaagac aataacatat gaaaaaaagt gtcatcgctg
      181 gcgtctttat tgctctgtca tttaccacgt gttcagctat cgcgaacagc cttgcattat
      241 cattagcaaa tgatgatgca gggaagtttc aaccaatact taatgatatt tatggcaata
      301 aacatgaaaa cagagatgat tactcacaag gcttatttct gggatatagc cacgatatct
      361 cagactcgag ccaattatct ctccatattg cgcaagatat ttactctcca tcaggcagta
      421 ataaaagaca caacacagct gtaactggag acagagcttt tagtgcatac actcacactg
      481 gtattgaatg gaactccctt gcgaatgact ggattcgcta tcgattaggt actgacatag
      541 gtgttgttgg ccccgacgca ggcggtcaga aagtacaaaa taaagctcat gagattattg
      601 gggcagaaaa atatcatgca tgggatgatc aaatagagaa tcgctacggt tatactgtaa
      661 aagggatgct atccatgaca ccaagtatgg atattttagg tgctaatgtt ggattatacc
      721 ctgaagtttc tgctgttact ggaaacttat ttcaatatgt agcatatggc gcaaccattg
      781 ccattggtaa tgataaaacc ttcaattcgg ataatggctt tggtctgctg gctccccgtg
      841 gtttaatgca tatgtccgat acaagcggat tcaaatacaa gatttttgca ggtatggaaa
      901 gacgagatgt caatcgcaac tatactctcg aaggaaaaac aatacagacg aaacaaacaa
      961 cagtatcgct aaacaaaact gttgatgaat atcaagttgg cgcaacaatt gggtatgcac
     1021 ctgtagcctt cacactagca tttaataaag taacatcaga attcaagaca ggggatgact
     1081 attcatttat aaatggagca atcaccttct tttt
//
LOCUS       DAZFWP020000423         1107 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFWP020000423 DAZFWP020000000
VERSION     DAZFWP020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.432"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1049
                     /gene="ehxD"
                     /locus_tag="ACHK0Z_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             <1..1049
                     /gene="ehxD"
                     /locus_tag="ACHK0Z_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HGU4505175.1"
                     /translation="VESDILKSEASLLYEKTEQYRYAILSESIQRNELPEIRITDFPG
                     GEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERDAALARVSLYEHQVSQ
                     EGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQVSQLEKEIELVREELA
                     LETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAPVSGTVQELNIHTEGG
                     VVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVDAYPYTRHGYLTGKVK
                     NITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVMAEIKTGVRSVISYLL
                     SPLKETINESLRER"
BASE COUNT          399 a          164 c          236 g          308 t
ORIGIN      
        1 gtgttgaatc tgatatttta aaatcagaag catctttgtt gtatgaaaaa acagaacaat
       61 atcgctacgc aattctttct gaatcaatac aacggaatga gcttcctgaa ataaggataa
      121 cagattttcc tggcggagaa gataatgcgg gaggtgaaca ttttcagagg gttagctcat
      181 taataaaaga gcaattcatg acatggcaga acagaaaaaa tcaaaagcag ctcacattaa
      241 ataaaaaaat agttgaacgg gatgcagcgc ttgctcgtgt tagcctgtat gagcatcagg
      301 tatcacagga aggaagaaaa ctcaatgatt ttaagtattt gttgaataaa aaagctgttt
      361 ctcaacattc agttatggag caggaaaata gctatattca ggcaaaaaat gaacatgcag
      421 tctggcttgc acaggtttct caacttgaaa aagaaataga acttgtgcgg gaagaactgg
      481 cactggagac gaatatcttc agaagtgaaa ttatcgagaa gcacagaaaa tcaacagata
      541 acattgtgtt gctggagcat gagcttgaaa aaaacaggca gagaaaagca tcatctttta
      601 ttaaagctcc tgtgagtggt actgttcagg agttaaatat acatacagaa ggtggtgttg
      661 tcacaacagc agaaacgctg atgattattg tccctgataa tgatattctc gaagtaacag
      721 catctgtact taacaaggat atcggtttta tacaacctgg acaggaggtc gttattaaag
      781 tagatgcata tccatataca cgtcatggtt accttacagg gaaagtaaaa aatataactg
      841 cagattctgt ttctgttccg gatacagggc ttgtatttaa cgtgattata tctgttgatc
      901 ggaatgatat acagggagaa agaaaaaaaa tccctgttac agctggaatg actgttatgg
      961 cagaaataaa aaccggggtg cgtagtgtta tcagctatct tcttagtcca ttaaaggaaa
     1021 ctattaatga aagtttacgt gaacgttagt atataaataa catattggct ttttatatga
     1081 tatagatttc tgaaatgatc ttgtaaa
//
LOCUS       DAZFWP020000424         1093 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFWP020000424 DAZFWP020000000
VERSION     DAZFWP020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.433"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..207)
                     /locus_tag="ACHK0Z_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             complement(<1..207)
                     /locus_tag="ACHK0Z_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505176.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQ"
     gene            complement(229..768)
                     /gene="yhaB"
                     /locus_tag="ACHK0Z_RS26895"
                     /old_locus_tag="pgaptmp_005379"
     CDS             complement(229..768)
                     /gene="yhaB"
                     /locus_tag="ACHK0Z_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGU4505177.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1024..>1093)
                     /locus_tag="ACHK0Z_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             complement(1024..>1093)
                     /locus_tag="ACHK0Z_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HGU4505178.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT          330 a          195 c          210 g          358 t
ORIGIN      
        1 ttgaaaatca ccagtgataa tgcattttcc acctggttgt aacgcgttaa gtaaagtgac
       61 catttgaata gaggtctgtt ttagtttttc tatacctggt gctagtgctt cgaggttatt
      121 aactatgggg ctttcaggaa gatcattcat ttttcgacgg actaaatcac tactaatatc
      181 attaccaatt gatgaaacag ggaacattat tttatccttt ataaataatc aataaaaatc
      241 attattaaaa tgatcgggtg aaaaaaagag aatttgccaa caatctgaag taatgctggt
      301 tttattaaag tgcgcaggag gtgttaagat ggcttctttt cgggtgaatt ttgcacaata
      361 cttagaaatc gtctccttaa tacattccag ctctttttta ttgacggctg agcaagtaca
      421 ttgttcacca aaaggtgcaa aagtgcctac atctaatgtt tgtatgatat aatcgtcatg
      481 ttctcctgta gaaatatcta tgatgaatgg actcatattt tccggttgga ttcggatcat
      541 gacagaagga acatcgcctt gatagaaatt caatgcagta attgcagata atactctccg
      601 cagttcatta cttatactgt aaattttatt aacgtcattg gtggagacaa tttctgcaaa
      661 ctttcttttt accgtccaat aatcaatatt tctattgtga ttgttaacca ctgcgtgcat
      721 tggcgggatt gaagggtgaa aaatatgtgc aattgggaag cctttcattg atgcctgcct
      781 tgtcgtcatg acaaacaata atagatgctt attttcctcg cagggttctt tcgcactatc
      841 aaaaaacgct catttacccg gcgttacagt aaacgaagca acattctgtg gcctacgacg
      901 atcaaaagct ctgtgaaaat ttcccttgtt gtctttgtct gaccttatcg cggcgatcag
      961 caattattcg ttttgtgatc ctcaacacaa aatgctattc gtcgcctatg aggggagaag
     1021 aatttatgga aaacgattgc attcacaaaa tgacgtcctg tcagaagaga aaaagttgac
     1081 gttaatcaat tgt
//
LOCUS       DAZFWP020000425         1084 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFWP020000425 DAZFWP020000000
VERSION     DAZFWP020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.434"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..111
                     /locus_tag="ACHK0Z_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /pseudo
     CDS             19..111
                     /locus_tag="ACHK0Z_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            124..213
                     /locus_tag="ACHK0Z_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             124..213
                     /locus_tag="ACHK0Z_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505179.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            233..>1084
                     /gene="nleL"
                     /locus_tag="ACHK0Z_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             233..>1084
                     /gene="nleL"
                     /locus_tag="ACHK0Z_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HGU4505180.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKN"
BASE COUNT          324 a          176 c          230 g          354 t
ORIGIN      
        1 ttggcgggat gaagtaaccc gacagcttca caaaaccgga gtccggctcc ggtttttgtt
       61 gtcatgtccg gggaatattt gttagataaa aaagaggaga taattcaata gggagttaaa
      121 ttaatgccga taaatctgac atcttatttg tgggtacagg gacagaaagt tgtcccggca
      181 gttgtgtttt ctaattttac tttagtggac taagtaaaaa ggagtgagat aaatgctgcc
      241 cactacaaat atctctgtaa attctggagt aatatctttt gaaagtcctg tagattcacc
      301 atctaacgag gatgttgaag ttgccctcga aaagtggtgc gctgagggag aatttagcga
      361 aaatcgtcat gaggttgcat caaaaatact tgatgttata agtactaatg gagagacttt
      421 atcaatcagt gagccaataa caacattacc agacttgctt ccaggttctc tgaaagaact
      481 ggttttgaat ggatgtacag agcttaaatc aataaactgc ttacccccca acttatcttc
      541 attaagtatg gttggatgct catcattaga ggttataaat tgcagcatac ctgaaaatgt
      601 cattaattta tctttatgcc attgtagttc tttgaaacat atagaaggtt cctttcctga
      661 ggcactcaga aattccgtat atttaaatgg ctgtaattca ttaaatgaat cgcaatgtca
      721 attccttgca tatgatgtca gtcaaggccg tgcctgcctg agcaaagctg agcttactgc
      781 tgacttaatt tggttgtcgg ctaaccgaac gggtgaagag tctgctgaag aattgaatta
      841 ctctggatgt gacttgtcag gtctaagtct tgtagggctg aatttatcat cagtaaattt
      901 ttctggagca gtgcttgatg atacagatct caggatgagt gatttgtctc aggctgtatt
      961 ggaaaactgt tcttttaaaa actcgatttt gaatgaatgt aatttttgtt atgctaattt
     1021 atctaattgt attattaggg ctttgtttga aaactctaat ttcagcaatt ccaatcttaa
     1081 aaat
//
LOCUS       DAZFWP020000426         1073 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFWP020000426 DAZFWP020000000
VERSION     DAZFWP020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.435"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1073
                     /locus_tag="ACHK0Z_RS26920"
                     /old_locus_tag="pgaptmp_005384"
     CDS             <1..>1073
                     /locus_tag="ACHK0Z_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505181.1"
                     /translation="NLKSTQMMSVIDAIGEGPIEGPVKGLQSILVNKTPLTDTDGNPV
                     IHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGV
                     QSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRP
                     FNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTV
                     NYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLG
                     AADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMP
                     VWNGQTLTFVQDRPSDVVWPYTN"
BASE COUNT          248 a          292 c          334 g          199 t
ORIGIN      
        1 aatcttaagt ccacgcagat gatgagcgtg attgatgcga ttggtgaggg accgatagaa
       61 ggtccggtga agggactgca gagtattctg gtgaacaaaa ccccactgac ggacacggac
      121 ggcaatcccg tgatacacgg tgtgaccgcg gtctggcgtg ccggggagca ggagcagaca
      181 ccaccggaag gctttgagtc ctccggagct gaaaccgcac tgggcgtgga agtgacgaaa
      241 gcaaagccgg tgacgcgcac cattacgtcc gcgaacattg accgcctgcg ggtcaccttc
      301 ggggtgcagt cactgttgga gaccacctca aagggcgacc gtaatccctc ttctgtccgc
      361 ctgctgattc agttacagcg taacggtaac tgggtgacag aaaaggacgt caccattaac
      421 ggcaagacca cctcacagtt cctggcctcg gtgattctgg ataatctgcc tccccggccc
      481 tttaacatcc ggatggtcag ggagacagcg gacagcacct cggaccagct gcagaataag
      541 acgctctggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg
      601 attgtggggc tgcaggtgga tgcggagcag tttggcggtc agcagatgac ggtgaactac
      661 catatccgag gtcgcatcat ccaggtaccg tcaaactatg acccggaaaa acgcacttac
      721 agcggcatct gggacggcag cctgaaaccg gcatacagca acaacccggc ctggtgcctg
      781 tgggacatgc tgactcaccc gcgctacggc atgggaaaac gtctgggggc ggcggatgtg
      841 gacaagtggg cgctgtatgc catcgggcag tactgcgacc agacggtccc ggatggtttc
      901 gggggcacag agccgcggat gacctttaat gcgtacctgt cacaacagcg taaggcgtgg
      961 gacgttctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg
     1021 ctgacgttcg ttcaggaccg cccgtcggat gtggtgtggc cgtacaccaa ctg
//
LOCUS       DAZFWP020000427         1070 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFWP020000427 DAZFWP020000000
VERSION     DAZFWP020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.436"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1070
                     /locus_tag="ACHK0Z_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             <1..>1070
                     /locus_tag="ACHK0Z_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505182.1"
                     /translation="AIGEGPIEGPVKGLQSILVNKTPLTDTDGNPVIHGVTAVWRAGE
                     QEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLLETTSKGDR
                     NPSSVRLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADS
                     TTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVP
                     SNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAI
                     GQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQD
                     RPSDVVWPYTNSDVVVDDNGVG"
BASE COUNT          237 a          285 c          346 g          202 t
ORIGIN      
        1 gccattggtg aagggccgat tgaaggtccg gtgaaggggc tgcagagtat cctggtgaac
       61 aaaaccccgc tgacggacac ggacggtaat cctgtgatac atggtgtgac agcggtctgg
      121 cgcgccgggg agcaggagca gacaccacct gaaggctttg agtcctccgg agctgaaacc
      181 gcactgggcg tggaagtgac gaaagcaaag ccggtgacgc gcaccattac gtccgcgaac
      241 attgaccgcc tgcgggttac cttcggggtg cagtcactgt tggagaccac ctcaaagggc
      301 gaccgtaatc cctcttctgt ccgactgctg attcagttgc agcgtaacgg taactgggtg
      361 acggaaaagg atgtcaccat taacggcaag accacctcgc agttcctggc gtcggtgatt
      421 ctggataatc tgccgccccg gccctttaac atccggatgg tcagggagac ggcggacagc
      481 accacggacc agctgcagaa taagacgctc tggtcgtcat acaccgaaat catcgatgtg
      541 aaacagtgct acccgaacac ggccattgtg gggctgcagg tggatgcgga gcagtttggc
      601 ggtcagcaga tgacggtgaa ctaccatatc cgcggtcgca tcatccaggt gccgtcaaac
      661 tatgacccgg aaaaacgcac gtacagtggt atctgggacg gcagcctgaa accggcatac
      721 agcaacaacc cggcctggtg cctgtgggac atgctgactc acccgcgcta cggcatggga
      781 aaacgcctgg gggccgcgga tgttgacaag tgggcgctgt atgccatcgg gcagtactgc
      841 gaccagacgg tcccggatgg tttcgggggc acagagccgc ggatgacctt taatgcgtac
      901 ctgtcacaac agcgtaaggc gtgggacgtt ctcagtgatt tctgctcggc gatgcgctgt
      961 atgccggtat ggaacggcca gacgctgacg ttcgttcagg accgtccgtc ggatgtggtg
     1021 tggccgtaca ccaacagcga tgtggtggtg gatgataacg gcgtggggtt
//
LOCUS       DAZFWP020000428         1045 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFWP020000428 DAZFWP020000000
VERSION     DAZFWP020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.437"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..74
                     /locus_tag="ACHK0Z_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /pseudo
     CDS             1..74
                     /locus_tag="ACHK0Z_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(490..>1045)
                     /gene="nleF"
                     /locus_tag="ACHK0Z_RS26935"
                     /old_locus_tag="pgaptmp_005387"
     CDS             complement(490..>1045)
                     /gene="nleF"
                     /locus_tag="ACHK0Z_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HGU4505183.1"
                     /translation="GSSANLYSWMYVSGRGNPSTPESVSELNHNHFLTPELQDKLDAM
                     ISIYSNARNSNELEEIYQELSAFVSGLMDKRNSVFEVRNENTDEVVGALREGMTIDDR
                     DGYIRELFFLYSLKVKIEESRQGKEDSKCKVYGLLCPHHSSELYGDLRAMKCLVEGCS
                     DDFDPFDIIRVPDLTYNKGALQCG"
BASE COUNT          315 a          250 c          176 g          304 t
ORIGIN      
        1 ccatgcatga cgtgtggtgc ctgttgtgca tttttcgcgt ctctttttac tgggctgaag
       61 cagatgatgc tggcgaaagg tttatcagaa aaccgtagcg ccttcttgca tacgacgtgt
      121 ccgcagtttt atataatccc gaccagaaac ctcttataaa aagtcgatac acaaatatca
      181 tggataatca ccaccttctg acgaagaata tctgatgaaa ttaatcgccc ctctcctgag
      241 accattgcgc tggggtaaat ttgtaactac gtgtgtacag tgaggtgtcg tatagcacgc
      301 cgtcaggaaa tgcaaggtgc ccccgatatc cccgactaca agtcagcaaa caaagtgatt
      361 ggctggcaat ccacatcaca gaaatggaca atgctacttt ataatcgagc ttggtagcca
      421 ccttgatggt cacttttaaa aaaagcgttt acacaaaatg ataagcaggc tcacaggacc
      481 tccctctaat catccacatt gtaaagctcc tttgttgtaa gtaagatccg gcaccctaat
      541 aatatcaaaa ggatcaaaat catcactgca tccttccaca agacatttca ttgctcgtag
      601 atcaccatat agctctgaag agtgatgcgg acataacaga ccataaactt tacatttaga
      661 atcttcttta ccttgtctac tttcctcaat ttttactttc aatgaataca gaaaaaaaag
      721 ctccctgata taaccatccc tgtcatctat cgtcatcccc tccctcagtg ctccgacaac
      781 ctcatcagta ttttcatttc tcacctcaaa tacactattt ctcttatcca tcagcccact
      841 tacaaaagca cttagctctt gataaatttc ctcaagctca ttactatttc tggcatttga
      901 gtatatagag atcatagcat caagtttatc ttgtaattca ggagtaagaa aatgattatg
      961 attaagttca cttactgatt ccggagtcga agggttacct cttcctgata catacatcca
     1021 tgaataaaga tttgctgaag agcca
//
LOCUS       DAZFWP020000429         1021 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFWP020000429 DAZFWP020000000
VERSION     DAZFWP020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.438"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..>1021
                     /locus_tag="ACHK0Z_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             120..>1021
                     /locus_tag="ACHK0Z_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HGU4505184.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNG"
BASE COUNT          271 a          192 c          271 g          287 t
ORIGIN      
        1 caccttccca cctccaacta acacttaata cagacctctg acaatttaat aagtttcatt
       61 tttaatgtct gacgactcat ctctttcgtg gctgtattgt ttgtatatgg agcgtttata
      121 tgaatagaat ctatcgcgtg atatggaatt gcactctaca ggtatttcag gcctgctcgg
      181 aattaactcg cagggcaggt aaaacatcga cggttaattt gcgtaaatcc tctggactga
      241 caacgaaatt cagtagattg acgctgggtg ttttgctggc actaagcggt tcagcgtctg
      301 gtgcaagtct ggaagttgat aatgatcaga ttaccaatat tgatactgat gttgcttatg
      361 atgcctacct ggttggctgg tatggcactg gagtgcttaa tattttggct ggcggtaatg
      421 cctccttaac cactattact accagcgtca ttggcgctaa tgaggactca gagggcaccg
      481 ttaatgtttt gggtggcacc tggcgattgt atgatagcgg aaataatgca aggcctttaa
      541 atgtgggtca atccggaacg gggacgctga atattaaaca gaagggtcac gtcgatggag
      601 gctatttaag attaggttct tcgacaggag gcgtcgggac ggtcaatgtt gagggagagg
      661 actctgttct gacgaccgaa ttattcgaaa tagggagtta cggtacgggc tcattaaata
      721 ttacggataa gggttacgtc acgagttcaa tcgtcgccat tttaggctat caggcgggca
      781 gtaatggcca ggttgtcgtt gaaaagggtg gcgagtggct aataaaaaat aacgattcct
      841 caattgaatt tcaaattggt aatcaaggaa ctggggaggc gactattcgc gagggtggac
      901 tgattacggc tgaaaatacg attatcggtg gcaatgccac cggtgtcgga accctgaatg
      961 tgcaggatca agactctgtc atcacggtac gcagactcta taatggatat ttcggtaatg
     1021 g
//
LOCUS       DAZFWP020000430         1004 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFWP020000430 DAZFWP020000000
VERSION     DAZFWP020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.439"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(29..>1004)
                     /locus_tag="ACHK0Z_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             complement(29..>1004)
                     /locus_tag="ACHK0Z_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4505185.1"
                     /translation="QTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGTHKVDGNQFE
                     ALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLADEL
                     VNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLATML
                     AGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEANGREQLAATLAAMPEMTV
                     EKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAAGS
                     GKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          178 a          339 c          263 g          224 t
ORIGIN      
        1 aacctcctgt tgatggtcag cactgacttc atggcatggc catgagcatt ttcacgtccg
       61 catcaccgtc tgctgacgtc tgtggcacgc caccctgtac cgcagccggt gaatggttcg
      121 ccatgatgcg ttcaaacagg gcggttgtgg atgcagagac cggttctgcc ttacctgatc
      181 ccgcagccag cacagcccgg gcgctctcca cagtcattcc cgggcaggca gccagctgtt
      241 cagcctgcgc ctcagcccct tttgcctcat ccagtgccat gatctgatca cggagtgagg
      301 gtccggcatc cgcctgcggt gaagcagcca ggatcgggcg ggctttttcc accgtcatct
      361 ccggcatcgc cgccagcgtt gccgccagtt gttcacgacc gttcgcttct tcacacgcca
      421 taatgcgatc ggcttcactc tgcgcggatg ccaccggctg ctgcggtgcc gccgcggcca
      481 gaatcgcccg ggcctgttca acgctcatgc cctgttgtcc tgccagcatc gtggcaagct
      541 gttcacgtcc tttcgcttcc tggcatgtca ggatccccat cactcgctgg ttctcctgcg
      601 cggcggcttc cgttgcagtt aattgcggca tagtgcctcc tctgacatta ctgttcagcg
      661 ccgtggccat cacactgatg gcatccgacg cattgactaa ttcatccgcc agcccggcat
      721 caatgccgga ctgaccttca aaaacggcgg cctctgttcc cgtgacggca tcaacagaca
      781 gaccggtaaa catcgccact ttttcggcaa acatccggcg cgccgcatca atgcgctgct
      841 gcatgtcctg gcgaacctct gccggtaagg cttcaaactg attgccatcc accttgtgcg
      901 tccctgagta aatcagcgtg atatccacac cggcctgcgc cagatgaccg gcatagctga
      961 catggctcat catcacgcca atggagccga tacgggatgt ctgg
//
LOCUS       DAZFWP020000431          993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFWP020000431 DAZFWP020000000
VERSION     DAZFWP020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.440"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..479)
                     /locus_tag="ACHK0Z_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             complement(<1..479)
                     /locus_tag="ACHK0Z_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4505186.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     "
     gene            complement(469..>993)
                     /locus_tag="ACHK0Z_RS26955"
                     /old_locus_tag="pgaptmp_005391"
     CDS             complement(469..>993)
                     /locus_tag="ACHK0Z_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4505187.1"
                     /translation="RYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFI
                     AARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQ
                     ESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAYQQQIA
                     ESRRPEEETTPRET"
BASE COUNT          214 a          331 c          248 g          200 t
ORIGIN      
        1 gccgtgtcat tgcacagtgc ccagaccggc ttctgctgac ggaggcggta aatcatgtca
       61 gcgcagtcaa acgcgccggc ggcctgcccg cccggactgt caatgtccag cagtacgccc
      121 cgcacctggc tatccgccat tgcctgctga agacaggcga caataccgtc atagcctgtc
      181 attccggaaa atggccgcat accccccagc cggtgcacca gcgtgccggt caccggcagt
      241 acagcaatac cgttcaccac cctgtaaaca cgggccggtc gtttacctcc ggccatgtac
      301 tcgtccgttt cagccagcat tccgggagca tcaaactgta cctgctgttg tggtaccgaa
      361 agacttgctg cccccatctc gcgcccgagc gcgcaaaaga aaacccgcgc ataggcgggc
      421 tccagaagca gcggttcatt gaatgctgcg gcaataatgt gtgaaagatt acgtctcacg
      481 tggtgttgtc tcctcttccg gcctgcgact ctccgctatc tgctgctgat acgcctgcgc
      541 tatccacacc ggacgtgaga gtccggcttt ttgccgctct gcagattccc tgacctgctg
      601 gcggaaaatg tcctgataat cctcgcccat cagcgccagc tctttctcat acgtgctcag
      661 tccggcctca atgcgcatca ctgattcctg aacctccttg agcccgtcaa tggccattct
      721 tccggcacca atccactctg cccgtgacca ggctgatcgc gcctgataaa aatcaaaacg
      781 cgcccgtggc ggacggataa tcccccgaag aagtgcctct tccagccagc aggaaaacat
      841 ctgcgtggcc agccgggccg caataaattt tcgccgcccc ataaaatagc gccacgactc
      901 attggcagag gccctggcac ttgaataact gaccttcgag taatcacggg acaactgttc
      961 gtaggaaacg ccaagaccgg cggcgatata ccg
//
LOCUS       DAZFWP020000432          990 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFWP020000432 DAZFWP020000000
VERSION     DAZFWP020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.441"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..740
                     /locus_tag="ACHK0Z_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             <1..740
                     /locus_tag="ACHK0Z_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4505188.1"
                     /translation="INLDSAFSLSEEERSYFEEKAKSLNALAQLGISVEIISHELEEM
                     DSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIM
                     DYVLKFFGERFERQRITIEFSEEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRL
                     IKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIW
                     YLEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            742..>990
                     /locus_tag="ACHK0Z_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             742..>990
                     /locus_tag="ACHK0Z_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HGU4505189.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDR"
BASE COUNT          320 a          161 c          192 g          317 t
ORIGIN      
        1 gtataaactt agattcagca ttctcattat ctgaagaaga acgctcatat tttgaagaaa
       61 aagctaaaag tttaaacgcg cttgcacagt taggtattag tgttgagata atatctcatg
      121 aacttgaaga aatggattct atggtaacca gaggactaaa ctctcttcct acttctgtaa
      181 aagaacaccc tggtttttca ttggcgttaa atgctcacag atcgcttact caacaaatac
      241 gtttcttatc acctttgaaa atatcaggtt atcaatccag gcagagaata actggaaaaa
      301 atatcatgga ttatgtcctg aagttctttg gggagcgttt cgaacggcaa cgaataacta
      361 ttgaatttag tgaagagttt aagcaaatcg caataacaga tataccatca aggatctatc
      421 ctgtttttac taatattatc aacaatgcaa tgtattgggt cagtctgtca aataataggc
      481 tcataaagat tggttttgtg aattctttgg ttatcatagc aaattctggt ccggcaattg
      541 ataccgatga tatcccgcga ctatttgaac tattttatag caaaagagca aatggacatg
      601 gggtaggtct gtatctatgt cgagaaaacc ttgctgttgc acatcataaa atatggtatt
      661 tagaacctga tgaaggcgat aactatttaa taaaagatgg cgctaatttt gtgatccagt
      721 tcaatggagt ggagttctaa tatgacagtg gcaaattata attctcttgt ccagaaaact
      781 ttctgcgaaa atgcaattcg ttccgttgtc atgattgatg acgattttct gacgtattct
      841 gaatcaatca gggcgttgaa taacgaagtt gatttagact acaacaaaat tgactcatct
      901 aaacgagccg ctactcttga gagctttttt caatctaaaa atatgatttg tgatgttgac
      961 aatggttctg ttaatttcga tgtggatcgg
//
LOCUS       DAZFWP020000433          988 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFWP020000433 DAZFWP020000000
VERSION     DAZFWP020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.442"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(31..249)
                     /locus_tag="ACHK0Z_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             complement(31..249)
                     /locus_tag="ACHK0Z_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGU4505190.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(275..649)
                     /gene="tomB"
                     /locus_tag="ACHK0Z_RS26975"
                     /old_locus_tag="pgaptmp_005395"
     CDS             complement(275..649)
                     /gene="tomB"
                     /locus_tag="ACHK0Z_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU4505191.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          289 a          200 c          197 g          302 t
ORIGIN      
        1 ccggttatag ctccgaaagc gaattattta ttagcgaata aatttccata ctgaggaagg
       61 gatcttgtcg tatagtttat tcatggtcaa ttcggcgagg cggtgatctg cggctgagta
      121 aaataccgcc agttcattat ctgataattc gtatttattt ttctcgataa cacgctccag
      181 cgtgtcaatt gtctggcaac gacgtaaacg cattaaataa tcggttttcg ttaaaggttt
      241 ttcggacata cttctaccca tggttgtaat aattctaaca agataaactc gcaggattct
      301 ctttcgtcgc attgacaaaa caacggaata gtcgattacc tgacttccgc catttctgaa
      361 gatcctgcat attaatacca taactgctga acaacataaa ggtgtcatcc agatattcgt
      421 cgatctgctc aatgagctta ttgtcttcat tatacttaat tttgtaatta agtgcgaagg
      481 tcgcaatatg ctcaatcagt tcattcaact ggaggttgat cgccgaggtt gggtcgttaa
      541 cccagccatg attgctttct tcaaggtttg caagacagtc atgatacagg gtttcacaga
      601 gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta ttcatccata acgcgtcccc
      661 ttcttagtgg ttgaactaac ggacaccttt cgggatggaa aaaacttact gacctggact
      721 tgccctctcg tttgttagct taactataag ccactctttg caggttttca tcgcattatt
      781 acgaaaaatt acaattagga aggaatcggg acaggactca ctttttcata ctagcactaa
      841 cccgcagcag gtaaaagcag tttgctcgaa ctgttcataa catttcagga ttatcgccag
      901 atgaaattgg tgacactaat accaggattg ctctgaatat gacgtaataa ccgaggaatg
      961 aataaagaat taccgcaaat aattaaga
//
LOCUS       DAZFWP020000434          986 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFWP020000434 DAZFWP020000000
VERSION     DAZFWP020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.443"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>986
                     /locus_tag="ACHK0Z_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             <1..>986
                     /locus_tag="ACHK0Z_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4505192.1"
                     /translation="RNGGWVTEKDITIKGKTTSQYLASVVVDNLPPRPFNIRMRRMTP
                     DSTTDQLQNKTLWSSYTEIIDVKQGYPNTALVGVKVDSEQFGSQQVSRNYHLRGRILQ
                     VPSNYNPQTRQYSGIWDGTLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALY
                     VIGQYCDQSVPDGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFV
                     QDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWETATELVE
                     DTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGL"
BASE COUNT          228 a          253 c          315 g          190 t
ORIGIN      
        1 cgtaatggtg gctgggtgac ggaaaaagac atcaccatta agggcaaaac cacctcgcag
       61 tatctggcct cggtggtggt ggataacctg ccgccgcgcc cgtttaatat ccggatgcgc
      121 agaatgacgc cggacagcac cacagaccag ctgcagaaca aaacgctctg gtcgtcatac
      181 accgaaatca tcgatgtgaa acagggctac ccgaacacgg cactggtcgg cgtaaaggtg
      241 gattcggagc agttcggcag ccagcaggtg agccgtaatt atcatcttcg cgggcgtatt
      301 ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat acagcggtat ctgggacgga
      361 acgttaaaac cggcatacag caacaacccg gcctggtgtc tgtgggatat gctgacccac
      421 ccgcgctacg gcatggggaa acgtcttggt gcggcggatg tggataaatg ggcgctgtat
      481 gtcatcggcc agtactgcga ccagtcggtg ccggacggct ttggcggcac ggagccgcgc
      541 attacctgta atgcgtacct gaccacacag cgtaaggcgt gggatgtgct cagtgatttc
      601 tgctcggcga tgcgctgtat gccggtatgg aacgggcaga cgctgacgtt cgtgcaggac
      661 cgaccatcag ataaggtgtg gacctataac cgcagtaatg tggtgatgcc ggatgatggt
      721 gcgccgttcc gctacagctt cagcgccctg aaggaccgcc ataatgccgt tgaggtgaac
      781 tggattgacc cgaataacgg ctgggagacg gcgacagagc ttgttgaaga tacgcaggcc
      841 attgcccgtt acggtcgtaa tgtcacgaag atggatgcct ttggttgtac cagtcggggg
      901 caggcacacc gagccgggct gtggctgatt aaaacggaac tgctggaaac gcagaccgtg
      961 gatttcagcg tgggcgcaga agggct
//
LOCUS       DAZFWP020000435          979 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFWP020000435 DAZFWP020000000
VERSION     DAZFWP020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.444"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..560
                     /locus_tag="ACHK0Z_RS26985"
                     /old_locus_tag="pgaptmp_005397"
     CDS             <1..560
                     /locus_tag="ACHK0Z_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4505193.1"
                     /translation="AKPRSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYE
                     NDARALFEFTSGVNVTESPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFR
                     LGGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDE
                     MVPEFIEKMDEALAEIGFVFGEQWR"
     gene            557..739
                     /locus_tag="ACHK0Z_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             557..739
                     /locus_tag="ACHK0Z_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU4505194.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPDLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            712..903
                     /locus_tag="ACHK0Z_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             712..903
                     /locus_tag="ACHK0Z_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4505195.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
BASE COUNT          257 a          220 c          267 g          235 t
ORIGIN      
        1 tagcaaaacc ccgctccgga aaaaagtggc ctgacatgaa aatgtcctac ttccacaccc
       61 tgcttgctga ggtttgcacc ggtgtggctc cggaagttaa cgctaaagcg ctggcctggg
      121 gaaaacagta cgagaacgac gccagagccc tgtttgagtt cacttccggc gtgaatgtta
      181 ctgaatcacc gatcatctat cgcgacgaaa gtatgcgtac cgcctgctct ccagatggtt
      241 tatgcagtga cggcaacggc cttgaactga aatgcccgtt tacctcccgg gatttcatga
      301 agttccggct cggtggtttc gaggccataa agtcagctta catggcccag gtgcagtaca
      361 gcatgtgggt gacgcgaaaa gatgcctggt actttgccaa ctatgacccg cgtatgaagc
      421 gtgaaggact gcattatgtc gtggttgagc gggatgaaaa gtacatggcg ggttttgacg
      481 agatggtgcc ggagttcatc gaaaaaatgg acgaggcact ggctgaaatt ggttttgtat
      541 ttggggagca atggcgatga agcatcctca cgataatatc cgggtaggtg cgatcacttt
      601 cgtctactcc gttacaaagc gaggctgggt atttcccgac ctttctgtta tccaaaatcc
      661 actgaaagcc cagcggctgg ctgaggagat aaataataaa cgaggggctg tatgcacaaa
      721 gcatctcctg ttgagttaag aacgagcatt gagatggcac atagccttgc tcaaattgga
      781 gtcaggtttg tgccaatacc agtagaaaca gacgaagaat ttcatacgtt agccacatcc
      841 ctttcacaaa agctggaaat gatggtggcg aaagcagaag cagatgagag agaccaggta
      901 tgacaaccac tgaatgcatt tttctggcag cgggcttcat attctgtgtg cttatgcttg
      961 ccgacatggg gcttgttca
//
LOCUS       DAZFWP020000436          973 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFWP020000436 DAZFWP020000000
VERSION     DAZFWP020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.445"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..488)
                     /locus_tag="ACHK0Z_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             complement(<1..488)
                     /locus_tag="ACHK0Z_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4505196.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VG"
     gene            465..607
                     /locus_tag="ACHK0Z_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /pseudo
     CDS             465..607
                     /locus_tag="ACHK0Z_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(802..915)
                     /locus_tag="ACHK0Z_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     CDS             complement(802..915)
                     /locus_tag="ACHK0Z_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4505197.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIVVLLWPVIKRNSLHNA"
BASE COUNT          245 a          284 c          234 g          210 t
ORIGIN      
        1 tgccccacgg ttccgtactg cccctttgac aggtccgctt tcggatggtt aaggcggctc
       61 atgtcctgca catcatgcag acaatggtcc gccggaatga tgtcgttata tttacaggcg
      121 acaccgcccg gtgtcaccgt actgcgacgc gccagctgct taatgcgcgg gtccggacgg
      181 tcatatgtct ccggcagcgg aaggccttca ccatatgcca tgctgtttga ctgccccgcc
      241 agaaccacaa caaagtaata ctccgggtct ctggtggcgc tgattactgt gccttctcca
      301 tccgacggct tcaccaacac aggtgtggtg acatcacctt ccgccgcaat ggcctgcatc
      361 agagtataag gcgtgatggc caccgggctg ccaaatggct gccacccctc cttcagtttt
      421 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc ataatgttta
      481 atcgacatcg aatttctccc gtgtacagga acagagttaa aaagccggaa ccggaatcaa
      541 attacaggat ggccatctgc cagtggctgg tcgtaaaaaa aggccacgcc atgcgcagcc
      601 ggaaataaag ggataacgat gatagtttga gaaaaacaga aacaacactt ttgtggcaaa
      661 gcatggtgcc gggtgcctcc cggtgaattc agtatcagca cctgaatccg cgattatccc
      721 atatacctgg ttgctgatcg cccctccgca cagggggatt caccatgcag aagtgttttt
      781 aataaacagc aaacaaaaaa atcaagcatt atgcaggctg tttcttttta tcaccggcca
      841 cagcaatacc acaatgccgc agaccagcac cccatccgcc agcaccgaca tgattctgct
      901 ggtgaaatcc accatcacca ccagaaacag caggagtgca gccacagcca ggcgcagttt
      961 taccgtcaca ggt
//
LOCUS       DAZFWP020000437          972 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFWP020000437 DAZFWP020000000
VERSION     DAZFWP020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.446"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..504)
                     /gene="sctT"
                     /locus_tag="ACHK0Z_RS27015"
                     /old_locus_tag="pgaptmp_005403"
     CDS             complement(<1..504)
                     /gene="sctT"
                     /locus_tag="ACHK0Z_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU4505198.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLE"
     gene            complement(506..766)
                     /locus_tag="ACHK0Z_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             complement(506..766)
                     /locus_tag="ACHK0Z_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4505199.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIEVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(776..>972)
                     /locus_tag="ACHK0Z_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             complement(776..>972)
                     /locus_tag="ACHK0Z_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4505200.1"
                     /translation="FYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMD
                     GWTMLSKGLILQYFDLSINP"
BASE COUNT          344 a          215 c          162 g          251 t
ORIGIN      
        1 ctctaattta ggaaaattaa aattgcctga aggccataaa ttataacttt gccaaagagt
       61 ttccagaata aagttcattc cacctttagt aagataaacc gcagcagaga aaaggttaaa
      121 taatcttgct aattccgagg tatcgacacc tgtagccgga tccaaagtac tacttaatgt
      181 tgcaccgcgt tggttatcga taaagctacc tatagcaagg aacatccaaa acggggatgc
      241 taataagcat gcaataaaca gccctatgat aatttctctg gcagcgaaca aagcaatatc
      301 gatttcattt aagttcaata attcatagtg gtaatgctga actaatgcac atgataccac
      361 tattattatt gggtgacgaa ctactgttgg tatattccca ctggcgaaaa atggtaacaa
      421 atagaaagtt ggtgccaatc ggcaaaaacc gagcgctgta gcagccagca gactatgcaa
      481 ctgatagagt atcgcctccc ccatgctatc ctctcgcgaa tgctaaattc agcatttcga
      541 taccaaatga atataatttt tcaccatacc acccgctcat taaaaaaaag caaatactta
      601 cgcacaataa tttcacgcca aatggcaacg tctgttcttg caactgcgtt accgtttgaa
      661 aaagccctac caataaacca acaaatgtcg caacttcaat aggtcctgca gacataacta
      721 aaatcagata cagcgcccta ttccccgcaa aaacaatatc gtccatattt tttcctcagg
      781 gattaatgga taggtcaaaa tattgtaaaa tgagcccttt tgataacatt gtccaaccgt
      841 ccatcgcgac aaacaaaatt aattttatcg gtgtagatat ggttaccgga ctcatcatca
      901 tcatacctaa cgttaacaga acgctggata taaccagatc cactacgaca aagggtaaat
      961 agatataaaa ac
//
LOCUS       DAZFWP020000438          966 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFWP020000438 DAZFWP020000000
VERSION     DAZFWP020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.447"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..458
                     /locus_tag="ACHK0Z_RS27030"
                     /old_locus_tag="pgaptmp_005406"
     CDS             102..458
                     /locus_tag="ACHK0Z_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4505201.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(509..>966)
                     /gene="yhaJ"
                     /locus_tag="ACHK0Z_RS27035"
                     /old_locus_tag="pgaptmp_005407"
     CDS             complement(509..>966)
                     /gene="yhaJ"
                     /locus_tag="ACHK0Z_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4505202.1"
                     /translation="IVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSE
                     VTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMV
                     EKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT          239 a          235 c          208 g          284 t
ORIGIN      
        1 aatatcaaaa cctttataac atccgaaaaa gaaagatacg aatagcattt acacaatacc
       61 cggaatagac tttcgtcact tttaattaaa gggatgtttt tatgcagtgg tatttagccg
      121 tattaaaaaa ttatgttggt ttctctggtc gcgcacgtcg taaagagtac tggatgttta
      181 ctctgattaa cgccatcgtc ggcgctatta tcaatgtcat tcaattgatt ttaggtttag
      241 agtttccatt cctttctctc atttacctgg cagcaacaat cattccggtc attgctctgt
      301 gtgttcgtcg tctgcacgat actgaccgtt caggtgcgtg ggcgcttctc tatctggtac
      361 cgatcattgg ctggttagtt ctgtttgtat ttgcctgttt agaaggcaat tctggcagca
      421 atcgttacgg aaacgatcct aagtttggtt caaattaatc ttagaattgg ggcgacattt
      481 cgcccctttt tattaataat aacaaatatt attttccgct aaaaagtttg ggaatttccc
      541 gcagacacca ggatttcgct tcccccatac tgtcacgacg ccaggccata ataatatcga
      601 tctcgctggt cgattccggg ctgacgacac gcaaccgccc ttccgcaata tctttttcga
      661 ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga cgtttatctt
      721 caatcgtgct caccgttaag cgcggctgtt tgtccagcag ctgtacggtc aacaccgggc
      781 gctcacgagc ggtatccgct accgcaattc cacgatattt cacgcgcgtc acttcagata
      841 acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg ttcattaagg
      901 tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc ggcgcgataa
      961 caatat
//
LOCUS       DAZFWP020000439          962 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFWP020000439 DAZFWP020000000
VERSION     DAZFWP020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.448"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(141..>962)
                     /gene="yfdF"
                     /locus_tag="ACHK0Z_RS27040"
                     /old_locus_tag="pgaptmp_005408"
     CDS             complement(141..>962)
                     /gene="yfdF"
                     /locus_tag="ACHK0Z_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HGU4505203.1"
                     /translation="GSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHD
                     INNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKG
                     DSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSK
                     AIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEII
                     STLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLSAPGVNFDDI"
BASE COUNT          268 a          182 c          164 g          348 t
ORIGIN      
        1 gttgttatcc ctcaattacc acaagtaaaa ggagataaac cgacaatgac ccggacgtaa
       61 tgatgtaaaa atactgatgt tcgtcactga ctatttccga tattcaggag ttgaaaaatc
      121 aaaaaactgg aaaaataatt ttaaatatca tcaaaattaa cacctggcgc agatagttga
      181 ttaatttcat tttgatattc cgtctggcga ttttgaagcc ttttttcaac tttatcttcg
      241 agatccttaa agtctttgta tttattttca ggatggtcaa cgtcaacttt gttagcgaat
      301 aaagtagaaa ttatttcttt tacattatcg cgtctaaatt ttgcatcgtc cacttgaaaa
      361 ttatatctta ttgctcgacc taaacgttta aaaacttcgt taggttctct cagtctttcc
      421 ccgttgagct cgtttacaac ctgatgatcg gaacctgttg caaatgattc tgccatgata
      481 gctttactgc tttcaaacca cgctgaagca ttcctggctt tttcactaca atattgggtg
      541 agactatcac cattaatacg aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa
      601 ccgagagata agtaatcatt tccgttttta tttttaatta caatagcgag agagtcgcct
      661 tttttatctt taccgtacca ggtaaataag tccgttggtt cgctattccc gctctcatca
      721 aattccgtta ccgtatgtct gagtttgtgt gatatcttat cgaagataaa accaggatct
      781 ccatcatata tttcttttaa tgcgctgaag atcatttcct tgttattgat atcatgagtc
      841 ttctcaacta tgttgtcatt aatgatagta cggacgagtt ctttgtcttt atagttcgca
      901 ggtaattcta ttggttcttg gttgtcgata ccaatagtaa tatggtctgt atcattggag
      961 cc
//
LOCUS       DAZFWP020000440          961 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFWP020000440 DAZFWP020000000
VERSION     DAZFWP020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.449"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            465..>961
                     /locus_tag="ACHK0Z_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             465..>961
                     /locus_tag="ACHK0Z_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4505204.1"
                     /translation="MAFKHYDVVRAVSPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLP
                     ETYDRPEPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVG"
BASE COUNT          226 a          242 c          273 g          220 t
ORIGIN      
        1 gtcacaattc agtcagttga cagttgcctg tcagactgag catttgttaa aaaaatttcg
       61 catggtgaat ccccctgtgc ggaggggcga ctggtgtata aggtgtcatc tctgacgaca
      121 acctggatcg agatacgcgg gttcagggac accggactga actcaccggg aggcacccgg
      181 caccatgcaa tggcacatag cgccactctc cagcccctct ccggaggggc tttcttatgg
      241 acaaaaaaag cccgcgctgg gagacgcggg cggcaaggaa taaacaacaa aacgtgaagt
      301 aatatttcag ctggcgaata atatccgaca gtaatcactc tgcgcaatag cgcggccttt
      361 ttcgtattgc gggctgttgt ctctcttctg ccattgtcct gtaacttccg gatttcagcc
      421 cgctcctcat tttactcaca atattatccc ggccgggagg attcatggca tttaaacact
      481 acgatgtggt cagggcggtg tcgccttcag accttgctga tgcacttgct caaaaaattc
      541 gtgaaggatg gcaaccatac ggcgggccgt tttcttcgta tacggatgat ggcgcagcac
      601 ttattcaggc gattgtcgca gaaggtgatg tgagcacacc tgttgtggtg aagccgacag
      661 gtggagaagg tgcagtaatc agtgccacca gcgacccgga gtattacttt gttgtggttc
      721 tggcagggca gtcaaacggc atgtcgtatg gtgaaggtct tccgctgccg gagacatatg
      781 accgtccgga gccgcgtatt aagcagctgg cgcgtcgcag tacggtgaca ccgggcggtg
      841 tcgcctgtaa atataacgac atcattccgg cggaccattg tctgcatgat gtgcaggaca
      901 tgagccgcct taaccatccg aaagcggacc tgtcaaaggg gcagtacgga accgtggggc
      961 a
//
LOCUS       DAZFWP020000441          958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFWP020000441 DAZFWP020000000
VERSION     DAZFWP020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.450"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..361
                     /gene="escR"
                     /locus_tag="ACHK0Z_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             <1..361
                     /gene="escR"
                     /locus_tag="ACHK0Z_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HGU4505205.1"
                     /translation="DEKILSPYRGFLEKNTEKENVEFFERAAQKKLGNETILKKDSLF
                     ILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILLF
                     ILVGGWQKLFEFLLVVN"
     gene            361..630
                     /gene="escS"
                     /locus_tag="ACHK0Z_RS27055"
                     /old_locus_tag="pgaptmp_005411"
     CDS             361..630
                     /gene="escS"
                     /locus_tag="ACHK0Z_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HGU4505206.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            630..>958
                     /gene="escT"
                     /locus_tag="ACHK0Z_RS27060"
                     /old_locus_tag="pgaptmp_005412"
     CDS             630..>958
                     /gene="escT"
                     /locus_tag="ACHK0Z_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HGU4505207.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISS"
BASE COUNT          281 a          156 c          181 g          340 t
ORIGIN      
        1 tgatgaaaaa atattatcac cataccgcgg ttttttagaa aaaaatacag agaaagaaaa
       61 tgttgagttt tttgaacgtg cagctcaaaa aaaattgggt aatgaaacta tcttaaaaaa
      121 agactctcta tttatactgt taccagcttt cacgatgggg cagcttgaag ctgcattcaa
      181 gataggtttt ttgctctatt taccttttat tgcgatagat ttgatcattt ccaatatctt
      241 attggccttg ggtatgatga tggtatcgcc agtaacaatt tcgattcctt ttaagatctt
      301 gttattcatt cttgttggag gctggcaaaa attattcgaa tttctgttgg tggtgaattg
      361 atggaaactg gatattttgt tcaattatgt gtgcaaacgc tttggataat atttatcctc
      421 tcactgccta cagtcatagc ggcctctgtt gtcggcatta ttattagcct ggttcaggct
      481 ataacgcagt tacaggatca aacattgcct tttttgctaa aaataatagc agtgtttgct
      541 acgcttgccc tgacttatca ctggatggga acaacaatca tcaacttctc gtcgataata
      601 tttgaaatga ttccgaaggt gaatggctaa tgaatgaaat aatgacggtc atagtatcat
      661 catttttttg tatactcagg ccgctgggga tgtttattat tctaccaata ttttcaacag
      721 gtgttttgct cagtaacttt atacgcaact caattatgat tgcgtttaca cttccaatta
      781 tagtagagaa ctatacattt tcggaaaaat taccatccgg aatttttcag ttgacaggca
      841 tagtgctaaa agaaataagt ataggctttt tcattgggtt atcatttact attctttttt
      901 gggcaataga tgcggctggg cagattattg atactttaag aggttcaaca atatcttc
//
LOCUS       DAZFWP020000442          941 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFWP020000442 DAZFWP020000000
VERSION     DAZFWP020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.451"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>941
                     /locus_tag="ACHK0Z_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             <1..>941
                     /locus_tag="ACHK0Z_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505208.1"
                     /translation="ELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLE
                     TQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAASRTLTLDREVTLPET
                     GAATVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLQSLRRRLFRCV
                     SIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADG
                     QYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLPLGEYTLTV
                     RAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQIT"
BASE COUNT          202 a          282 c          297 g          160 t
ORIGIN      
        1 gaactggtgg aagacccgga agccatactg cgctacggac gcaacctgct gaagatggac
       61 gcgttcggct gtaccagccg cggtcaggcc caccgtgccg gactgtgggt gataaagacc
      121 ggactgctgg aaacgcagac ggtggatttc acgctcgggt ctcaggggct gcggcacaca
      181 cccggtgaca tcattgaaat ctgtgataac gactatgccg ggaccctgac cggcggacgt
      241 gtcctgtcca ttgatgccgc cagccgcacc ctgacactgg accgtgaggt gaccctgccg
      301 gagacaggtg ccgccacggt gaacctgatt aacggcagcg gtaagccggt gagtgtggac
      361 atcaccgcac accccgcgcc ggaccggata caggtcagca ccctgcctga tggtgtggag
      421 acatacggtg tatggggact ctccctgcag tcactgcgtc gtcgcctgtt ccgctgtgtc
      481 tccatccggg aaaacacgga cggcaccttt gccatcacgg cggtgcagca cgtaccggaa
      541 aaggaagcca tcgtggataa cggggccagc tttgagccgc agtcaggcac cctgaacagc
      601 gtcatccctc cggcagtgca gcacctgacg gtggaggtga gcgcagctga cggtcagtat
      661 ctggcacagg cgaaatggga cacgccgcgg gtggtgaagg gtgtgcgctt cagtctgcgc
      721 ctgaccagcg gaagcggaga agacagccgt ctggtgacca ccgccatcac tgcggataca
      781 gagcaccgtt tcagtggtct gccgctcggg gaatacaccc tgacagtcag ggcaattaac
      841 agttatggcc agcagggcga accggccacc accacgttca ggattaatgc acctgcggta
      901 cccgccacga ttgagctgac accgggctat tttcagataa c
//
LOCUS       DAZFWP020000443          930 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFWP020000443 DAZFWP020000000
VERSION     DAZFWP020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.452"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..803
                     /locus_tag="ACHK0Z_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             <1..803
                     /locus_tag="ACHK0Z_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGU4505209.1"
                     /translation="HNANNESRVAQLQGGSVVFIHYKSTEPEDLGKLLIVMVDKQSAY
                     DFDSDKLTPTRLNPINTDALRQAAMFDLTLFEASYPENKGDSYVHFLQGKSKSDFFKD
                     SLGCRHDSDNKRSIQQLFSAIDIFASKNSLGRVLRDTIDNEVKSLLEKKSKDKHGNKS
                     VKIEDISKIIDSCLTDSHKCKGTFVDFVNLNGFQIDPQFEPTPKAAESALTIEVADND
                     NNFKLKIMRGAIGDEKSNKPVILTDNKCEIVIKLSQANYDELKRYRDS"
BASE COUNT          330 a          158 c          184 g          258 t
ORIGIN      
        1 aacataatgc taataacgaa tcgagagttg cccaacttca gggggggagc gttgttttca
       61 tccactataa gtctaccgaa ccagaggatt tggggaaact tctaatcgta atggttgata
      121 aacaaagcgc ctatgacttt gattcggata agttgacccc aacaagatta aatccaatta
      181 ataccgatgc cttacggcag gctgcgatgt tcgatttaac tttattcgaa gccagttatc
      241 cagaaaacaa gggtgactca tatgtacatt tcttgcaagg taaatctaaa agcgattttt
      301 tcaaagactc attaggttgc cgacatgatt cggacaataa aagaagcatt cagcaattat
      361 tcagtgctat agatattttt gctagtaaaa actcactcgg tcgtgtactg cgtgatacta
      421 ttgacaatga agttaaatcc ttactggaaa aaaaatcaaa agataaacac ggaaataagt
      481 ccgttaagat agaggatatt tcaaaaataa ttgactcatg cctgactgat tcacacaaat
      541 gtaagggaac gtttgtcgat tttgttaatc ttaatggttt ccagattgac cctcaatttg
      601 aaccaactcc taaagcagct gaaagcgcgc ttacaattga agtggcagat aacgacaata
      661 attttaagtt gaaaataatg cgtggagcta ttggagacga aaagtcaaat aagcctgtaa
      721 ttctcactga caacaaatgc gaaattgtga taaaattgag tcaggcaaat tatgatgaac
      781 tcaaaagata tagagacagc taataatgac aattgctgat gacttatcaa gattagcgca
      841 gattattaac ggagcctcaa gcagagttga gggctactac actgtcataa gtcttgaaga
      901 aagcattgtt attgtaaata gttctgaaat
//
LOCUS       DAZFWP020000444          929 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFWP020000444 DAZFWP020000000
VERSION     DAZFWP020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.453"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..810
                     /locus_tag="ACHK0Z_RS27075"
                     /old_locus_tag="pgaptmp_005415"
     CDS             <1..810
                     /locus_tag="ACHK0Z_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4505210.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          271 a          209 c          262 g          187 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcc
//
LOCUS       DAZFWP020000445          921 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFWP020000445 DAZFWP020000000
VERSION     DAZFWP020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.454"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..558
                     /locus_tag="ACHK0Z_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             <1..558
                     /locus_tag="ACHK0Z_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4505211.1"
                     /translation="SYHPPICGEIHFGMVARLLREKGVCEFVEAAKIIKKKYPMVRFS
                     IAGDFDDNPGGIDAAQIVNWKAEGYVEFLGPCSYIKEYLSDISVFVLPSYREGVPKST
                     QEAMAIGRAIITTDVPGCRETVIDGYNGYLVPPWDTQKLALVMETFIHERELIVTMGL
                     NSRRFALSKFNESESANSLVNIITA"
     gene            637..>921
                     /locus_tag="ACHK0Z_RS27085"
                     /old_locus_tag="pgaptmp_005417"
     CDS             637..>921
                     /locus_tag="ACHK0Z_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGU4505212.1"
                     /translation="MAILVTGGAGYIGSHTVLMLLKEQYEVIVLDNFHNSSVESLHRV
                     KEITGSDICIINGDIRDRVILKSIFTENSITDVIHFAGLKSVSDSIQFPTE"
BASE COUNT          289 a          126 c          197 g          309 t
ORIGIN      
        1 agttatcatc caccaatttg tggggagatc cattttggta tggtggcaag attattgaga
       61 gagaagggcg tatgtgaatt tgttgaagcg gctaaaataa taaagaaaaa atatccaatg
      121 gtccgattta gtatagctgg tgattttgat gataatccgg gtggaattga tgctgcacaa
      181 attgtaaatt ggaaagcgga aggttatgtt gaatttttag gaccgtgctc ttatataaaa
      241 gaatatttga gtgatatcag cgtctttgta ttaccttctt atcgagaagg tgtccccaaa
      301 agtactcagg aagcaatggc aataggtcgt gcgattatta cgaccgatgt gccaggttgt
      361 cgagaaacag taattgatgg atataatgga tatcttgtac ctccatggga tacacaaaaa
      421 cttgctttag ttatggaaac atttattcat gaacgtgagc ttattgtcac tatgggatta
      481 aatagtcgaa gattcgcatt gtcaaaattt aatgaaagtg aatctgcaaa ttctttagtt
      541 aatattataa ctgcataaga gttattaaat tatccaagct ttaataacat tctatctgct
      601 agtcttgttg ctttgattaa atgagggtgc tgttgtatgg ctatattagt gacgggtggt
      661 gctggatata ttggctcaca tactgtatta atgctgctga aagaacaata tgaagtaata
      721 gtgcttgata attttcataa ttcttcagtt gaatctttgc atcgcgttaa agaaattact
      781 ggtagtgata tctgtatcat taatggtgat attcgagatc gtgttatttt aaaatccatc
      841 tttactgaaa acagcataac agatgttatt cattttgctg gcctgaaatc agtaagcgat
      901 tcaattcaat tcccaacaga g
//
LOCUS       DAZFWP020000446          914 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFWP020000446 DAZFWP020000000
VERSION     DAZFWP020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.455"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>914
                     /locus_tag="ACHK0Z_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             <1..>914
                     /locus_tag="ACHK0Z_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4505213.1"
                     /translation="TPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEICYR
                     YDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADG
                     SVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGP
                     DGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQ
                     LLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEY
                     NIAGDLTAVIAPDGSRNGTQYDAWGKAI"
BASE COUNT          228 a          256 c          284 g          146 t
ORIGIN      
        1 ggacgccgcg cggcgaactg gcggtggtgt atgaccgtag tggcaaacag gtgcgcagct
       61 ttacttacga tgataaatac cggggccgga tggtggcgca ccgtcacacg ggccggccgg
      121 aaatctgtta ccgttacgac agcgacgggc gggtgacaga acagctaaac ccggcaggct
      181 taagttacac gtatcagtat gaaaaagacc gcatcaccat caccgacagc ctgaaccgcc
      241 gtgaagtgct gcacacgcag ggcgaagccg ggctgaagcg ggtggtgaaa aaggaacacg
      301 cggacggcag cgtcacgcag agtcagtttg acgcggtggg caggcttaag gcacagacgg
      361 atgccgcagg ccggacaaca gaatacagcc cggatgtggt gacgggcctc accacgccgg
      421 atggcagggc atcggcgttt tactataacc accacaacca gttaacgtca gccaccgggc
      481 ctgacgggct ggaattgcgc cgggaatatg atgaatccgg ccgtctgatt caggaaactg
      541 cccctgacgg cgatatcacc cgctaccgtt atgataatcc acacagtgac ttaccctgcg
      601 caacggaaga tgccaccggc agccggaaaa ccatgacgtg gagccgttac ggtcagttgc
      661 tgaccttcac tgactgttcc ggttatgtaa cccgctatga ccatgaccgt tttggtcagg
      721 tgacggcggt gcaccgcgag gaaggcctga gccagtacca cgcatacgac agccgtggac
      781 agttaactgc ggtgaaagac acgcagggcc atgaaacgcg gtatgaatac aacatcgccg
      841 gtgacctgac cgccgtcatt gccccggacg gcagcagaaa cgggacacag tacgatgcgt
      901 ggggaaaggc catc
//
LOCUS       DAZFWP020000447          913 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFWP020000447 DAZFWP020000000
VERSION     DAZFWP020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.456"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>913
                     /locus_tag="ACHK0Z_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             <1..>913
                     /locus_tag="ACHK0Z_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HGU4505214.1"
                     /translation="RDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDAVIAGQNQ
                     AYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFSKFRQDTIIPE
                     ALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTTGTGKYDWLT
                     PESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSTLKTENLPELFLTKIDTDTFFSQW
                     STKTKKDLQKKIQDLTVRYNELIDSSTIDFKNLYEIDQMLHMIMLEMNDDIAKRSLFS
                     LQVQIAEKIRRMTIPVDNIINIYPDLHK"
BASE COUNT          322 a          160 c          180 g          251 t
ORIGIN      
        1 tcgagacaca atgcccatcc ttcgtcgtta tcacctatgg cctgatggat ggaatattcg
       61 tgggctcaat ggattcatgc tatcacataa aggtagtgaa gtgattgatg ctgttatcgc
      121 aggccagaat caggcttaca gggaactaag aagaataaga gataatattc atagtgaaat
      181 atacttcaaa caaactgatg aattgtcctc acttccagat acagacaaaa ttggagggat
      241 tctggtaaaa aaataccttt caggaagtct tttttcaaaa ttcagacaag atactattat
      301 cccggaggca ttgagcaccc tccagatatc aggtcctgac ttgattcaaa gaaagatgtt
      361 gcaatttttc aggagtagag gggtgttagg tgaagagttc attaatgaaa gaaaactgag
      421 tgataaagct tatattggtg tctacaaaac aactggcaca gggaaatatg actggttaac
      481 ccctgaatcg atcggcgtta atgatgtcac gcccgcagat gaaagtacct ggtgtatagg
      541 aaaaggccgg tgtgttgatg acttcctgtt caaagatgtt tcaacactaa aaacagaaaa
      601 tcttccagaa ttattcttaa caaaaataga tactgatacg tttttctctc agtggtcaac
      661 caaaaccaag aaagatctgc aaaaaaaaat acaagacctt actgtacgtt ataatgagct
      721 aattgactcg tcgactatcg actttaaaaa tctatatgaa atagatcaaa tgctccatat
      781 gattatgcta gagatgaatg atgatatagc caaaaggtca ttgttttcat tgcaagttca
      841 aatagccgaa aaaattcgga ggatgaccat tcctgtagac aatataatta acatctatcc
      901 tgatctacat aaa
//
LOCUS       DAZFWP020000448          911 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFWP020000448 DAZFWP020000000
VERSION     DAZFWP020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.457"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..75
                     /locus_tag="ACHK0Z_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             <1..75
                     /locus_tag="ACHK0Z_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505215.1"
                     /translation="EGIRITDIDTSGIFDSDDMAIKAA"
     gene            148..288
                     /locus_tag="ACHK0Z_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             148..288
                     /locus_tag="ACHK0Z_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4505216.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NG"
     gene            281..424
                     /gene="kil"
                     /locus_tag="ACHK0Z_RS27110"
                     /old_locus_tag="pgaptmp_005422"
     CDS             281..424
                     /gene="kil"
                     /locus_tag="ACHK0Z_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4505217.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIH"
     gene            500..796
                     /locus_tag="ACHK0Z_RS27115"
                     /old_locus_tag="pgaptmp_005423"
     CDS             500..796
                     /locus_tag="ACHK0Z_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4505218.1"
                     /translation="MNAYYIQDRLEAQSWARHYQLIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            802..>911
                     /locus_tag="ACHK0Z_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             802..>911
                     /locus_tag="ACHK0Z_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4505219.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKG"
BASE COUNT          263 a          213 c          210 g          225 t
ORIGIN      
        1 gaaggaataa gaattaccga tatcgataca tcaggaatat ttgattcaga tgatatggct
       61 atcaaggccg cctgagtgcg gctttaccgc ataccaataa cgcttcactc gaggcgtttt
      121 tcgttatgta taaataagga gcacaccatg caatatgcca ttgcagggtg gcctgttgct
      181 ggctgccctt ccgaatcttt acttgagcga atcacccgta aattacgtga cggatggaaa
      241 cgccttatcg acatacttaa tcagccagga gttccaaaaa atggataaaa cacttatggc
      301 tatccagact aaattcacta tcgccacttt tattggcgat gaaaagatgt ttcgtgaggc
      361 cgtcgacgct tataaaaaat ggatattaat acagaaactg agatcaagca aaagcattca
      421 ctaaccccct ttcctgtttt cctaatcagc ctggcatttc gcgggcgata ttttcacagc
      481 cattttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag
      541 ctgggcacgt cactaccagc tgatcgcccg tgaagagaaa gaggcagaac tggcagacga
      601 catggaaaaa ggtcttccac aacatctgtt tgaatcactc tgcatcgatc atttgcaacg
      661 ctgcggggcc agcaaaaaag ccattacccg tgcgtttgat gacgatgttg agtttcagga
      721 gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat
      781 tgattcagag gtataaaacg gatgagtaca gcactcgcaa cgctggcagg gaagctggct
      841 gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg
      901 gcatttaaag g
//
LOCUS       DAZFWP020000449          895 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFWP020000449 DAZFWP020000000
VERSION     DAZFWP020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.458"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..403
                     /locus_tag="ACHK0Z_RS27125"
                     /old_locus_tag="pgaptmp_005425"
     CDS             <1..403
                     /locus_tag="ACHK0Z_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4505220.1"
                     /translation="GERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETGAAGPVGA
                     TGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGALITGLTFLDPKDATRVQGF
                     FQHLQVRFGDGPWQDVKGLDELGSDTGRTGE"
     gene            405..674
                     /locus_tag="ACHK0Z_RS27130"
                     /old_locus_tag="pgaptmp_005426"
     CDS             405..674
                     /locus_tag="ACHK0Z_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4505221.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(801..>895)
                     /locus_tag="ACHK0Z_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             complement(801..>895)
                     /locus_tag="ACHK0Z_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EspF repeat-containing protein"
                     /protein_id="HGU4505222.1"
                     /translation="QNEQSRPLPDVAQRLMQHLAEHGINTSKRS"
BASE COUNT          243 a          191 c          280 g          181 t
ORIGIN      
        1 gggagagcgg ggagagaccg gtctgacggg aaatgcaggt ccacagggtc caaagggaga
       61 taccggtgcg gcaggcccgg caggcccaca gggaccgaaa ggagaaacag gtgcggctgg
      121 cccggtgggg gcaaccggac ctcagggacc gaagggcgac ccgggggaga cacaaatacg
      181 gttccgtctg gggccgggaa acattattga gacaaacagc aatggctggt tcccggatac
      241 agatggcgca ctcatcaccg gactgacctt tcttgacccc aaagatgcca cacgggttca
      301 ggggtttttt cagcatttgc aggtcaggtt tggtgacggg ccgtggcagg atgtcaaggg
      361 gctggatgaa ttgggcagtg atacaggcag aacaggagaa tgacatgaat atactaaaaa
      421 aacttatgca gcgtctgtgt ggttgcggaa agcatgatgg ccgtgaacac gtgcagtcgc
      481 ttacagcaca actgcgactg gggccggcag acatcctgga gtcagatgag aatggcatta
      541 ttccggagca ggacagggta atcacacagg tggtgatact ggatacagat aaaaagctga
      601 tacagtgtgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg gaaaatattg
      661 gcgggatgaa atagcccgac agcttcacaa aaaccggagt ccggctccgg ttttttgttg
      721 gttagatgta atctgacaga tacctgtata aataaccggt aactgtcagg tcagagctaa
      781 tacaggtaat tatattataa tcacgagcgc ttagatgtat taatgccatg ctctgcaaga
      841 tgctgcatca gacgctgagc cacatcaggc agaggtcttg attgttcatt ttgta
//
LOCUS       DAZFWP020000450          894 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFWP020000450 DAZFWP020000000
VERSION     DAZFWP020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.459"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..62
                     /locus_tag="ACHK0Z_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /pseudo
     CDS             1..62
                     /locus_tag="ACHK0Z_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            62..223
                     /locus_tag="ACHK0Z_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /pseudo
     CDS             62..223
                     /locus_tag="ACHK0Z_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(273..626)
                     /gene="cmi"
                     /locus_tag="ACHK0Z_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     CDS             complement(273..626)
                     /gene="cmi"
                     /locus_tag="ACHK0Z_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HGU4505223.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYNCEEGYVEYAND"
BASE COUNT          280 a          182 c          152 g          280 t
ORIGIN      
        1 ttaaattact tgataaagtt attatctatc aacaacagaa tgagcaatta tatccagcac
       61 aggcgttgtt cgttcatatg atgataaata cgattttaac gccagcactc accgtggcat
      121 catcggagag tcgctcacaa ggctcggggc gatgttttct ggtaaagagt accagatact
      181 gcttcctggt gaaattcaca ttaaagaaag tggtaagcga taatataagg gccgggattc
      241 cggccttttt atgcagcctt tataacagtg tattagtcat tcgcatattc aacatatccc
      301 tcctcacaat tgtatatgcc ataatacatt ctcttaccct ccgggccttc agctttaaat
      361 gggacaagaa gtgttttttc atcttcaaca tctgttaaac ttctctcaat cttttcccat
      421 gtaattttcg ggatttctgt accaagaagt tgaacatcgt tgtcccagcg tttcattctt
      481 tgcaggtaat cattttttac aaacgttctg gcctgttcat cgctgacctg atagcatgcc
      541 ggacctttat cttcagacca gaaaaaaaca gcagcaagag caataatcgt taaaataaaa
      601 ataaatttca tgctaattac tttcataaaa tctccacaac ttctgtcgtt cattcgatat
      661 aaaaaacatt acgaatatat ccgtacaatg tgagcatact gtgaagtggc tttaacaatt
      721 atgaaataat acaatagatc acatattgca gtggtctctt ttttatagaa gaaatcacaa
      781 ttcataattg tgacttatta tgcatgtttg tttttcagca ttgtgaaccg ccccggaaat
      841 cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata ctcg
//
LOCUS       DAZFWP020000451          882 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFWP020000451 DAZFWP020000000
VERSION     DAZFWP020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.460"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            71..856
                     /locus_tag="ACHK0Z_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             71..856
                     /locus_tag="ACHK0Z_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505224.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          288 a          178 c          194 g          222 t
ORIGIN      
        1 gtggagtata taattaaaaa tcgttagcta aacattacca tgattatgac aggcgaggga
       61 gtgtccaatt atgaattcaa aaaagctttg ttgcatatgt gtgttattct cgctgcttgc
      121 aggatgtgcc tctgaatctt ctattgatga aaagaagaaa aaagctcaag tcacacaaag
      181 taatattaat aaaaacactc cccagcaact gacagacaaa gatttattcg gtaatgaaac
      241 cactctggcc gtatccgaag aggatattca agctgcgctt gatggagatg agttccgcgt
      301 tccccttaat tctcctgtaa tacttgttca atccggcaac cgcgcaccgg aaaccattat
      361 gcaggaggag atgcgaaaat attatactgt ttccacattc tctggtatcc cggacaggca
      421 aaagcctctg acttgtaaca aaaacaaaga taaaaatgag aacgaggatg ttgccagtgc
      481 tgagaatatg aactggatgc aggcactgcg ttttgtggct gcaaaagggc atcagaaagc
      541 gattattgtt ttccaggata cgttgcagac aggaaaatat gactctgcgc tgaaatcaac
      601 agtatggtcc gactataaaa atgaaaaact caccgacgct atctccctgc gctacctggt
      661 acgtttcacg ctggtggatg tggcaacagg tgagtgggct acctggtcgc cggtgaatta
      721 tgaatataaa gtgcttccac cattgcccga caagaatgaa gccagtacga ctgatatgac
      781 agagcagcaa atcatgcaac ttaagcaaaa gacctataaa gcgatggtaa aagatttggt
      841 taaccgctat caataataaa ttatatctgc cgccaggaat tt
//
LOCUS       DAZFWP020000452          880 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFWP020000452 DAZFWP020000000
VERSION     DAZFWP020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.461"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>880
                     /locus_tag="ACHK0Z_RS27160"
                     /old_locus_tag="pgaptmp_005432"
     CDS             <1..>880
                     /locus_tag="ACHK0Z_RS27160"
                     /old_locus_tag="pgaptmp_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505225.1"
                     /translation="VIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTRT
                     ITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTTS
                     QFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVG
                     LQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLW
                     DMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRKA
                     WDVLSDFCSAMRCMPVW"
BASE COUNT          199 a          241 c          278 g          162 t
ORIGIN      
        1 gtgatacacg gtgtgaccgc ggtctggcgc gccggggagc aggagcagac accgccggaa
       61 ggttttgagt catccggctc tgaaaccgca ctgggcgtgg aagtgacgaa ggcaaagccg
      121 gtgacgcgca ccattacgtc cgcgaacatt gaccgcctgc gggttacctt cggggtgcag
      181 tcactggtgg agaccacctc aaagggtgac cgtaacccgg catccgtccg cctgctgatt
      241 cagttacagc gtaacggtaa ctgggtgaca gaaaaggacg tcaccattaa cggcaagacc
      301 acctcacagt tcctggcctc ggtgattctg gataatctgc ctccccggcc ctttaacatc
      361 cggatggtca gggagacggc ggacagcacc acggaccagc tgcagaataa gacgctgtgg
      421 tcgtcataca ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc cattgtgggg
      481 ctgcaggtgg atgcggagca gttcggcggt cagcagatga cggtgaacta ccatatccgc
      541 ggtcgcatca tccaggtgcc gtcaaactat gacccggaaa aacgcacgta cagtggtatc
      601 tgggacggca gtctgaaacc ggcatacagc aacaacccgg cctggtgtct gtgggacatg
      661 ctgactcacc cgcgctacgg catgggaaaa cgtctggggg cggcggatgt ggacaaatgg
      721 gcgctgtatg ccattgcgca gtactgcgac cagacggtgc cggatggttt cgggggcaca
      781 gagccgcgga tgacctttaa tgcatacctg tcacaacagc gtaaggcgtg ggacgtcctc
      841 agtgatttct gctcggcgat gcgctgtatg ccggtatgga
//
LOCUS       DAZFWP020000453          876 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFWP020000453 DAZFWP020000000
VERSION     DAZFWP020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.462"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(495..584)
                     /locus_tag="ACHK0Z_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /pseudo
     CDS             complement(495..584)
                     /locus_tag="ACHK0Z_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          248 a          155 c          193 g          280 t
ORIGIN      
        1 ttgtacttta ttctctttca ttggtacttc ctcgctttaa aaaagagcgc acttcgtaag
       61 tgcccttata taaataacga gtttggtcaa ccaatttttt gacatgtatc acaaatttga
      121 atggatgtat tacatcggct atcttttatt gcaccaacgt cattgatata tgtcgcctga
      181 agtcagttcc gggaatgagt ctgatctcaa gactggccca gtccgggcgt tgattggcgc
      241 tgaggagcat atcgcatctc atcataatgt cgtatctcct ggggtgttat acaagatatc
      301 gttgttggtg acctgggaga ggaattgagt tctattaaac cgtcaactat gccggataca
      361 tactggatta cactgcaggc acgccttacg agagaacgtg ccgcagtgac gggttaatta
      421 tctgaaagaa tttgcgaggc tgtatcggtt actcattggt ttgatagttt tactctcggg
      481 agagtaatag atatttaatt cattaacgga aaccagtcag ttcctttcga tgcctgaatt
      541 tgatcccata gtttatccgg aatggttaat tcatgaacaa gttgtgttat cgttcatgag
      601 aagcataacg taaagggaaa agctcgatta gacggcagga tttgtcaggg gttatgaacg
      661 aaattcataa atctgtttga gtgttgcgat gggtagtgca agttcgatat ctccgcaatt
      721 tacagtctga tgaatgaaaa tgaatatcca taaaaaatat attggtttat cctggcgtat
      781 atacctactt cgatgtattg ccaatcgtgg taaattaaag acaggtcatt attattcact
      841 ctgaataatg aattattcac tgtccgcaga gtaaga
//
LOCUS       DAZFWP020000454          863 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFWP020000454 DAZFWP020000000
VERSION     DAZFWP020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.463"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..461)
                     /locus_tag="ACHK0Z_RS27170"
                     /old_locus_tag="pgaptmp_005434"
     CDS             complement(<1..461)
                     /locus_tag="ACHK0Z_RS27170"
                     /old_locus_tag="pgaptmp_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4505226.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQG"
     gene            complement(458..>863)
                     /locus_tag="ACHK0Z_RS27175"
                     /old_locus_tag="pgaptmp_005435"
     CDS             complement(458..>863)
                     /locus_tag="ACHK0Z_RS27175"
                     /old_locus_tag="pgaptmp_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4505227.1"
                     /translation="FEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNM
                     AVTGFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKW
                     QRRTHSAWRHRHWHASAFTGICNDLAAASACT"
BASE COUNT          178 a          275 c          235 g          175 t
ORIGIN      
        1 ccctgggcaa tcatgttgtc cagcgacgaa aaatacgtgt tctgtttacc gttatccgta
       61 cttttgtact ccggcgtctt tgccttcggg gccagcatct gggccacacc gcccagtatc
      121 atgctggccc ccagtgaaaa cagcatcgtg gtggcagaaa aaccgccggc tgccagggct
      181 gaaccccata acgccatcgt tgcgccggcg gtgaagaaag agcccacgat ggctgccgcc
      241 cccagcacaa tctgcagtcc gcctttccct gcaccggcca gtcgcggcac aatgtggatg
      301 accgttccct cacccagctg ttcgtgaaga cgggcgtaca ccgcctccgg tgccgtgtcc
      361 tcaccgcgaa tacgtatctg gtaccagcct tcgttcatct gacggcggaa tcccggcacc
      421 tgtaacgaca gcgcccggat ggcttccgct gccgtgttca cgtacaggct gaggcggcgg
      481 ccaaatcgtt gcaaatcccc gtgaaggcag atgcgtgcca gtggcggtga cgccaggctg
      541 aatgcgttcg tcgttgccat ttttcggaat acctctcccg tttactcagt tgttcaggca
      601 gatggtgaag cagttcaccg ttgccgcagt aaatggcggc atgattggcc accgatgcgc
      661 caaagcagca cagcaggata tcgcccgcct gtgcagagga caggggcacc cggtaaaagc
      721 cggtgaccgc catattgtcc aggtaaaggt tctgaccgtt gcgccaccag tcatcctcac
      781 gctcaaaatc cggcatatca attcccgcca gatggtatgc atcccggaac agcgtgtaac
      841 agtccgtcac cccgtgctca aag
//
LOCUS       DAZFWP020000455          862 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFWP020000455 DAZFWP020000000
VERSION     DAZFWP020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.464"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            238..765
                     /gene="sodC"
                     /locus_tag="ACHK0Z_RS27180"
                     /old_locus_tag="pgaptmp_005436"
     CDS             238..765
                     /gene="sodC"
                     /locus_tag="ACHK0Z_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4505228.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          217 a          236 c          202 g          207 t
ORIGIN      
        1 atcggtccct caccaatcgc atcaatcaca ctcatcatct gcgtggattt gagattatcc
       61 ttcgcctctc gaggcgtgtg cgccctgccg ccacctttgc ccataatgtt cctctcaatt
      121 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
      181 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      241 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      301 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      361 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      421 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      481 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      541 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      601 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      661 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      721 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      781 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      841 catcctccct ctctgagtta at
//
LOCUS       DAZFWP020000456          839 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFWP020000456 DAZFWP020000000
VERSION     DAZFWP020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.465"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..755
                     /gene="yciT"
                     /locus_tag="ACHK0Z_RS27185"
                     /old_locus_tag="pgaptmp_005437"
     CDS             6..755
                     /gene="yciT"
                     /locus_tag="ACHK0Z_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU4505229.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT          223 a          205 c          198 g          213 t
ORIGIN      
        1 cagcgatgaa ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta
       61 gcgtaaccga tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca
      121 acaccctcga aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata
      181 gtgatgacgt cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt
      241 ttgccgcgtc actggttcaa ccgggcgaaa ccatctttat cgaaaatggt agcagcaatg
      301 ccctgctcgc ccggactctg ggcgaacaga agaaaaatgt cactatcatc acagtcagca
      361 gctacattgc gcatttgctg aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt
      421 accagaaaaa aagcgaaagt atggttggcc ctttgacacg ccagtgcatc cagcaggtac
      481 atttcagcaa agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc
      541 gcgatatgat gcgtaccgat gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag
      601 tcctgactga cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc
      661 gattcaatcg cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc
      721 acagtaaact caccgttcac gtcgttgaca tttaaccatt tcccttctct acggatgatt
      781 tgcagtttgg caaatcatcc gctctaagat gattcctggt tgataattaa gactattta
//
LOCUS       DAZFWP020000457          839 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFWP020000457 DAZFWP020000000
VERSION     DAZFWP020000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.466"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..170
                     /locus_tag="ACHK0Z_RS27190"
                     /old_locus_tag="pgaptmp_005438"
     CDS             <1..170
                     /locus_tag="ACHK0Z_RS27190"
                     /old_locus_tag="pgaptmp_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4505230.1"
                     /translation="ADILESDENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRA
                     DGTWENIGGMK"
     gene            347..>839
                     /gene="nleB2"
                     /locus_tag="ACHK0Z_RS27195"
                     /old_locus_tag="pgaptmp_005439"
     CDS             347..>839
                     /gene="nleB2"
                     /locus_tag="ACHK0Z_RS27195"
                     /old_locus_tag="pgaptmp_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HGU4505231.1"
                     /translation="MLSPIRTTFHNSANIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISK"
BASE COUNT          279 a          123 c          209 g          228 t
ORIGIN      
        1 cggcagacat tctggagtca gatgagaatg gcattattcc ggagcaggac agggtaatca
       61 cacaggtggt gatactggat gcggataaaa agcagataca gtgcgtggta agaccgctgc
      121 aaattctgcg tgctgacggg acgtgggaaa atattggcgg gatgaagtaa cccgacagct
      181 tcacaaaacc ggagtccggc tccggttttt gttggcatgc catggtgatg tttgttagga
      241 aagcaaaggt ggcaaaactg ctggaggttt tgtggttgag tatgccaata taattaatag
      301 attaaagagt tagttgtgaa gaaaatatgg ataaacagga cgacgaatgc tttcaccgat
      361 aaggacaact ttccataact cagcaaatat agtgcagagt tcaccctgtc aaacggtttc
      421 ttttgccgga aaggaatatg agttaaaggt cattgatgaa aaaacgccta ttctttttca
      481 gtggtttgaa cctaatcctg aacgatataa gaaagatgag gttccaatag ttaatactaa
      541 gcagcatccc tatttagata atgtcacaaa tgcggcaagg atagagagtg atcgtatgat
      601 aggtattttt gttgatggcg atttttcagt caaccaaaag actgcttttt caaaattgga
      661 acgagatttt gaaaatgtaa tgataatcta tcgggaagat gttgatttca gtatgtatga
      721 cagaaaacta tcagatattt atcatgatat tatatgtgaa caaaggttac gaactgaaga
      781 caaaagagat gaatacttgt tgaatctgtt agagaaagag ctgagggaaa tttcaaagg
//
LOCUS       DAZFWP020000458          837 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFWP020000458 DAZFWP020000000
VERSION     DAZFWP020000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.467"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            29..>837
                     /locus_tag="ACHK0Z_RS27200"
                     /old_locus_tag="pgaptmp_005440"
     CDS             29..>837
                     /locus_tag="ACHK0Z_RS27200"
                     /old_locus_tag="pgaptmp_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4505232.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHP"
BASE COUNT          268 a          180 c          151 g          238 t
ORIGIN      
        1 tggaagaaaa atataaccgg agtagtgtat ggccaatctc tacgacttga aaaagttcga
       61 cttaaacctt cttgtcatat ttgagtgtat ttaccagcat ttaagcatta gcaaagctgc
      121 tgaatcacta tatatcaccc catcagccgt gagccagtcg ttacaacgct tacgtgcgca
      181 atttaatgac cctttattca ttcgctcagg aaaaggtatc gcgccgacga caacagggtt
      241 gaatctgcat caccatcttg agaaaaacct caaaggcctt gaacaaacaa ttaatatagt
      301 caataagtcc gaactaaaga aaaactttat tatttacggc cctcagctca tttcttgctc
      361 taataatagt atgctgatcc gctgcctacg gcaagatgcc tctgtcgaga ttgagtgcca
      421 tgatatcctc atgtcagcag agaatgctga agaactgttg gttcaccgca aagccgatct
      481 ggtgatcacc caaatgccag tgatcagccg ttccgtcatt tgcatgccat tgcatacaat
      541 tcgcaatact ctaatttgta gtaataagca tccgcgaatt actgacaata gcacttatga
      601 acaaattatg gcagaagagt tcacccaatt aatatccaaa tcagcaggag ttgatgatat
      661 tcaaatggaa atagacgaga agtttatgaa tcgcaaaatt tcatttcgtg gttcctcttt
      721 gctgacaatc attaatagca ttgcagttac agatttactg ggaatagtcc cctatgaact
      781 ttataattcc taccgtgact ttcttaattt aaaagagata aaactagaac atccgtt
//
LOCUS       DAZFWP020000459          832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFWP020000459 DAZFWP020000000
VERSION     DAZFWP020000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.468"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(361..>832)
                     /locus_tag="ACHK0Z_RS27205"
                     /old_locus_tag="pgaptmp_005441"
     CDS             complement(361..>832)
                     /locus_tag="ACHK0Z_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HGU4505233.1"
                     /translation="EALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMG
                     SHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDP
                     QVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          299 a          186 c          106 g          241 t
ORIGIN      
        1 tcaattatca cattcaaaaa aaatactgac aacaaagata ctcttcagac aaagttgtca
       61 tatgtaaatt ttcccttaaa acgttgctca aaaaaaatat aaaaatcata tgccctaatc
      121 aacatcctca agcactacga tatatgcagt atcctattca tatattacca ccatatcaga
      181 caaatcgaat cacaacgagt tctatcgtac tctttcatct tcgacactgt aaattttaag
      241 aacattcggc tcatgccact aaaccaagac ataataacaa cggagcaata aatgttccca
      301 ctgaacatgt gcaaacctaa aaagacagtg ttacaataat attcatccat ggtctctaaa
      361 tcaacgccac gcaacaggat aatacaatga aaatatttca ggaaaagatt cttttgcatg
      421 ctccgttagc atatcagtca gcacagcggt acaagtaaaa acattatttc ttccttgtag
      481 cctgtctaaa atatcattgt aataataaac tgagccaaat acctgaggat ctgttttata
      541 agcttgttca atcaatccat ttgcatatct ccgtagaatc tcaggagaat catactctgt
      601 accaaacata tcactcgatg agtatttaca gaaaaccccc cctaaacaaa ataatatttc
      661 agcctgtttt cttagtggca tatctttcaa tacatggctc cccataatca tatctacatg
      721 ctgagcgttc agttcccaac catcaagaaa gggattccag acacaggcaa gtttcctttg
      781 ctgatcatca gcaatcattt tataatctga tttattggaa ttaagtgcct ca
//
LOCUS       DAZFWP020000460          818 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFWP020000460 DAZFWP020000000
VERSION     DAZFWP020000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.469"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(62..280)
                     /gene="ydfC"
                     /locus_tag="ACHK0Z_RS27210"
                     /old_locus_tag="pgaptmp_005442"
     CDS             complement(62..280)
                     /gene="ydfC"
                     /locus_tag="ACHK0Z_RS27210"
                     /old_locus_tag="pgaptmp_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4505234.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(352..651)
                     /locus_tag="ACHK0Z_RS27215"
                     /old_locus_tag="pgaptmp_005443"
     CDS             complement(352..651)
                     /locus_tag="ACHK0Z_RS27215"
                     /old_locus_tag="pgaptmp_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505235.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          177 a          210 c          186 g          245 t
ORIGIN      
        1 gcactccacc tcacccgtgg agaactcctt aattaccaac cttagcttcg ttggttagct
       61 attaacgcgg gtatgtaatc attctggcaa tgcttaatgc cgctgctttt tccagattgg
      121 tgatatcctg ctccagagcg gacagatttt cagcctgctt agccctggct tcattggccc
      181 atttcaggtc ctgcgctgcc ttaattttct ggtgcatcca ctcataaagt tcatcatcgg
      241 tatagtctgg cgcgatgatg acgggttctc gtttctgcat gtcggctcct tgtggttagc
      301 gctgcctgct tttaaccacg tcaggcgagg tggtatcctc tgaggggtct gttactcgag
      361 aggaaattgg ttatgaatac aatcaagttt tcttgcccag aatgtggtgg cgaagtcttt
      421 gacacatcct ttaaaccgca tggctctgac agtttcgcgg gagccatctg caaaaattgt
      481 ggtcaccttg taactgaaga tgagtcctcg cagtttgatg acgaaatcgt tgacaatatc
      541 ttcggtgcac tcaccagaga ctttctgaag taaaggcgca taccgcttag ttaccgctct
      601 gataattctt acctgtccgg caatggcgct gatatcaata taaagcgcca tcgctgtttc
      661 tttgctgatc cctggatgcc ttccattctg atgtttgact tcgcccactg agaaatcctc
      721 tgtttcccct taacgccggg gtagcggaac aaaaaacctg ctgcatagtt aaacgtaatc
      781 cccgccgtca tgttcatacg cctcaggctg gctactta
//
LOCUS       DAZFWP020000461          815 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFWP020000461 DAZFWP020000000
VERSION     DAZFWP020000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.470"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            107..703
                     /gene="fimE"
                     /locus_tag="ACHK0Z_RS27220"
                     /old_locus_tag="pgaptmp_005444"
     CDS             107..703
                     /gene="fimE"
                     /locus_tag="ACHK0Z_RS27220"
                     /old_locus_tag="pgaptmp_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU4505236.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          230 a          157 c          188 g          240 t
ORIGIN      
        1 atcactccgt gtgtggttat ctttttatct attgggctaa ttttgaccga ttgaggtttc
       61 ctataggtat tcattcaaat atatctcagt taggagtact actattgtga gtaaacgtcg
      121 ttatcttacc ggtaaagaag ttcaggccat gatgcaggcg gtttgttacg gggcaacggg
      181 agccagagat tattgtctta ttctgttggc atatcggcat gggatgcgta ttagtgaact
      241 gcttgatctg cattatcagg atcttgacct taatgaaggt cgaataaata ttcgccgact
      301 gaagaacgga ttttctaccg ttcacccgtt acgttttgat gagcgtgaag ccgtggaacg
      361 ctggacccag gaacgtgcta actggaaagg cgctgaccgg accgacgcta tatttatttc
      421 tcgccgcggg agtcggcttt ctcgccagca ggcctatcgc attattcgcg atgccggtat
      481 tgaagctgga accgtaacgc agactcatcc tcatatgtta aggcatgctt gtggttatga
      541 actggcggag cgtggtgccg atacccgttt aattcaggat tatctcgggc atcgaaatat
      601 tcgccatact gtacgttata ccgccagtaa tgctgctcgt tttgccggac tatgggaaag
      661 aaataatctc ataaacgaaa aattaaaaag agaagaggtt tgatttaact cattgataat
      721 aaagttaaaa aacaaataaa tacaagacaa ttggggccaa actgtccata tcataaataa
      781 gttacgtatt ttttctcaag cataaaaata ttaaa
//
LOCUS       DAZFWP020000462          810 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFWP020000462 DAZFWP020000000
VERSION     DAZFWP020000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.471"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            43..318
                     /gene="yddL"
                     /locus_tag="ACHK0Z_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /pseudo
     CDS             43..318
                     /gene="yddL"
                     /locus_tag="ACHK0Z_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            357..>810
                     /locus_tag="ACHK0Z_RS27230"
                     /old_locus_tag="pgaptmp_005446"
     CDS             357..>810
                     /locus_tag="ACHK0Z_RS27230"
                     /old_locus_tag="pgaptmp_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4505237.1"
                     /translation="MITDLILHNNPRMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESINFPQGRN"
BASE COUNT          291 a          158 c          138 g          223 t
ORIGIN      
        1 tttagaaaat acgtttaacg ataattaaaa ggattctgtt aaatgaaatt aaaaatagtt
       61 gcggtggttg taactggttt gttagctgcg aacgtagcac acgctgccga agtctataac
      121 aaggatggta ataaactcga cctttatggc aaggttaccg ctctacgtta ttttactgat
      181 gataagcgtg acgatggtga taaaacttat gcccgtctcg gctttaaagg agaaacgcaa
      241 atcaatgatc aaatgattgg ttttggtcac tgggaatatg attttaaagg ctataacgat
      301 gaagccaacg gttcgcgcgg caacaatctc tgattgagtt aagtgcccat cacaatatga
      361 tcactgacct tatattacac aataatccca ggatgaaaac aatcacttta aacgacaacc
      421 atattgcaca tttaaacgcc aaaaacacta cagaactgga atatttaaac ttaagcaata
      481 acaatttact gccaaccaat gacattgatc aactaatatc atcaaaacat ctttggcatg
      541 tattagttaa cggcatcaac aatgatccac ttgcacaaat gcagtactgg actgcagtaa
      601 gaaatataat tgatgacact aatgaagtga ccattgattt atcaggactt aatttaacca
      661 ctcaaccacc agggctgcaa aacttcacct ctatcaatct tgataataac caactcacac
      721 attttgatgc aaccaactac gatagactcg taaagctaag tctgaatagt aatactcttg
      781 agtcaataaa ttttcctcaa ggcagaaatg
//
LOCUS       DAZFWP020000463          795 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFWP020000463 DAZFWP020000000
VERSION     DAZFWP020000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.472"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..679)
                     /locus_tag="ACHK0Z_RS27235"
                     /old_locus_tag="pgaptmp_005447"
     CDS             complement(<1..679)
                     /locus_tag="ACHK0Z_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4505238.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGR"
BASE COUNT          236 a          183 c          181 g          195 t
ORIGIN      
        1 ttcgccccca gttcagagtg aggtccacgt ctcccggcgt catcgtatac acaggagcag
       61 tttcagacag tgcctgacga aattctctct gtatctgcct gaagcgtaag gcttctgccg
      121 tgacagtgac aaaacgcaga actgctctgg atgcatctct ggtcattgta ttaccactga
      181 actccattaa cgccagatat gatgaaacca gtgagtgacg actgatttgc attccggaac
      241 gttccagcgc tgcgacacgt tgcagagtgg tataactgct gtccgttgtc atggaaaccg
      301 ttgtcacatc gggcactgat atatgtgtaa aatctgaaaa acggtagaaa gtatttgttg
      361 ccgtattaac gaacccggcc acatataaat tattttgctc aataatcaga cgaagatggt
      421 caaaacgcgc ctgatagaca tcaagccctc gtatatccac agcaaaataa ctgcccggtg
      481 gggtgtggtt aataacagac accgatgtgg tcccctgaga tatatgttca agaggggtcg
      541 atatctctgt ccgtatacta tttaacgaag agacataact ttgttgggtc gaaaagtcta
      601 tcgtaaactc ccgggaatag gataccgaag aaaaacccag taacaggcac agtacccatt
      661 taaataatat acacttcata tacaggtgtt ccttttggct gaagtaatca gcaccagacc
      721 cggcgcagat ataaaaaagg cccgcaaaag cgagcctggt aaataaatat ggcgcgttgt
      781 actggattcg aacca
//
LOCUS       DAZFWP020000464          794 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFWP020000464 DAZFWP020000000
VERSION     DAZFWP020000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.473"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>794
                     /locus_tag="ACHK0Z_RS27240"
                     /old_locus_tag="pgaptmp_005448"
     rRNA            <1..>794
                     /locus_tag="ACHK0Z_RS27240"
                     /old_locus_tag="pgaptmp_005448"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          222 a          177 c          245 g          150 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac
      181 tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag
      241 gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa
      301 cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc
      361 gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg
      421 ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg
      481 ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga
      541 ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg
      601 gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg
      661 gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat
      721 agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag
      781 ctgcggcagc gacg
//
LOCUS       DAZFWP020000465          788 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFWP020000465 DAZFWP020000000
VERSION     DAZFWP020000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.474"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            22..393
                     /gene="ler"
                     /locus_tag="ACHK0Z_RS27245"
                     /old_locus_tag="pgaptmp_005449"
     CDS             22..393
                     /gene="ler"
                     /locus_tag="ACHK0Z_RS27245"
                     /old_locus_tag="pgaptmp_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HGU4505239.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
     gene            408..626
                     /gene="escE"
                     /locus_tag="ACHK0Z_RS27250"
                     /old_locus_tag="pgaptmp_005450"
     CDS             408..626
                     /gene="escE"
                     /locus_tag="ACHK0Z_RS27250"
                     /old_locus_tag="pgaptmp_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HGU4505240.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRIIEIMLIRINNK"
     gene            639..>788
                     /locus_tag="ACHK0Z_RS27255"
                     /old_locus_tag="pgaptmp_005451"
     CDS             639..>788
                     /locus_tag="ACHK0Z_RS27255"
                     /old_locus_tag="pgaptmp_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system filament chaperone"
                     /protein_id="HGU4505241.1"
                     /translation="MSQTKNRELLDKKIRSEIEVIKKIIAEFDVVKENVNALSEKAKT
                     DPQAAE"
BASE COUNT          328 a          124 c          163 g          173 t
ORIGIN      
        1 agcatgcgga gattatttat tatgaatatg gaaactaatt cgcacacaac aagcccatac
       61 attcagctta ttgagcaaat tgaagtgtta caacaggaag caaagcgact gcgagagcag
      121 gaaattcaaa gtgtaattga gtcgattcaa aagcagatta cttattacaa tataacccta
      181 caagagctgg gatatactaa tgtgcctgat gatggccttg ctcgccggaa ctcatcgaaa
      241 ggagtttatt atcgcaatga agaagggcag acctggtcgg gagttggccg acagccacgc
      301 tggcttaaag aagcactgtt gaatggaatg aagaaagaag attttcttgt gaaggacacc
      361 gaagacgaaa taataccgct gaaaaatatt taacatgaaa tcattaaatg ataacgataa
      421 ctgaactgga agacgaaata ataaaaaata aagaagccgc aaatattttc attgaaaaaa
      481 taaacgacaa aaagaacgaa atccatgaaa aaatgaaaca ccccttggat aaagttacct
      541 acaatgaagc aaaggaactg ctcattgcct gcgatgcggc aatcaggatc atagagataa
      601 tgctaatcag aattaataat aaatagagga cgagtattgt gagccaaaca aaaaatagag
      661 aactgttaga taaaaaaata agatcagaaa ttgaggtgat aaaaaaaata atcgccgaat
      721 ttgatgtcgt aaaagaaaac gttaatgctt taagtgaaaa agcaaaaaca gatcctcagg
      781 ctgcagaa
//
LOCUS       DAZFWP020000466          787 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFWP020000466 DAZFWP020000000
VERSION     DAZFWP020000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.475"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            13..231
                     /gene="yiiE"
                     /locus_tag="ACHK0Z_RS27260"
                     /old_locus_tag="pgaptmp_005452"
     CDS             13..231
                     /gene="yiiE"
                     /locus_tag="ACHK0Z_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU4505242.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            450..692
                     /gene="yiiF"
                     /locus_tag="ACHK0Z_RS27265"
                     /old_locus_tag="pgaptmp_005453"
     CDS             450..692
                     /gene="yiiF"
                     /locus_tag="ACHK0Z_RS27265"
                     /old_locus_tag="pgaptmp_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGU4505243.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT          243 a          131 c          204 g          209 t
ORIGIN      
        1 aaggtggtgg taatggcgat gaatacggtt tttcttcatt tatcagaaga ggcaattaaa
       61 cggctgaaca aacttcgggg ctggcgtaag gtttcacgat ctgcaatttt acgcgaagcg
      121 gttgagcaat atctggagcg gcagcaattt ccggtgcgta aagcaaaggg aggcaggcaa
      181 aagggggagg tagttggtgt tgatgatcag tgtaaggagc ataaggaatg aaaaaaattt
      241 ttcattcttg aatataaaaa aacagatgcc cttattctgg tattaataca aggctgtttt
      301 acttgaactt ataataactg caactgttac atcgtatctg gaaaacgcct cgcaaaaata
      361 aaaaatgatg cgtaaatgag ctttctttaa agattcggca agggtacttt tctctccata
      421 gattcaaaaa tgatattggc gagatattta tgaactctct ggcaggtata gatatgggca
      481 gaattttact cgatttatcg aatgaggtga ttaagcaact tgatgatctc gaggtgcagc
      541 gtaatcttcc tcgtgcggac ctattaaggg aagcggtaga tcaatacctg ataaatcaat
      601 cgcaaacagc aagaaccagt gttcctggca tctggcaagg gtgtgaggaa gatggtgtcg
      661 aatatcagcg taagctgcgc gaggaatggt aatgggaaaa aatgatgtca atcagattgc
      721 tgacaacgtg cgcgttgttc atgccggatg cggcgtgagc gccttatcag gcctacaaag
      781 tcatgca
//
LOCUS       DAZFWP020000467          786 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFWP020000467 DAZFWP020000000
VERSION     DAZFWP020000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.476"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..733
                     /locus_tag="ACHK0Z_RS27270"
                     /old_locus_tag="pgaptmp_005454"
     CDS             <1..733
                     /locus_tag="ACHK0Z_RS27270"
                     /old_locus_tag="pgaptmp_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4505244.1"
                     /translation="KKSPQEYSLSFIKQLPNTVNLSSLTYNKEDDFLYATQNSPAQLL
                     KIARSGDIIGRAPLPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISLRNKI
                     QLEKFNKKNRGFEGLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQVTIPEELND
                     IHVKDISGLDFNNESLMILSDESRKLLKFNLTEMSFVEMMDLTKGNHSLTSDLLQPEG
                     IVTLPDESIYVASEPDILAKFVPNK"
BASE COUNT          258 a          165 c          149 g          214 t
ORIGIN      
        1 caaaaaatca ccacaggaat actcactttc atttataaaa caattaccaa atacggtgaa
       61 cttatcatct cttacgtata ataaagagga tgatttcctg tatgccaccc agaatagccc
      121 tgcgcagcta cttaaaatag ccagaagtgg cgacatcatc ggccgagccc cgctgccttt
      181 tatctctgat gccgaaacca ttgagcacat acaagggaac atttttgctg cagtagacga
      241 aaagacttct gaactattct tttttacagt tacaaaagat atgcatatct ccttgcgaaa
      301 taaaattcag ttagaaaaat tcaataaaaa aaatagaggg tttgaaggac tggcatggaa
      361 agcagaaggc cggatgctgt ttgtcgctaa agaacgacgc cctacaggca tgtttgccta
      421 tcagctttct ccagatctgc tgagtgcaaa acaagtaacg attccagagg aactaaatga
      481 tatacatgtt aaagatatat ccggcctgga ctttaataat gaaagtctga tgattctgtc
      541 cgatgaatcg cgtaagttac tcaagtttaa cctgacagaa atgtctttcg ttgagatgat
      601 ggacctgaca aaaggaaatc attctctgac cagtgatttg ctccaacctg aaggcattgt
      661 aactctgcct gatgaaagca tttatgtcgc tagtgaacct gatatcctcg ctaaatttgt
      721 ccctaataaa taagtctgtc actagcctta gtattgagcg accaaatctg gcaatctcat
      781 gaaact
//
LOCUS       DAZFWP020000468          776 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFWP020000468 DAZFWP020000000
VERSION     DAZFWP020000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.477"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            49..633
                     /locus_tag="ACHK0Z_RS27275"
                     /old_locus_tag="pgaptmp_005455"
     CDS             49..633
                     /locus_tag="ACHK0Z_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HGU4505245.1"
                     /translation="MPANISGTPFNSFGISFIQKQSCWRKSDDILRCSMGQRTIKLST
                     NTLNNRILTSVARQSTKDINAWKRDEQTVYPSRVINQGIDKYCAENSRNISSEVRQRV
                     FKFIEKDYSLKLNIIAAQSSINHLIIGNGRFGDKINMLCKGVSREVKNQTMDVIANQL
                     ADQFFQKHISPDVDIKQLRDDIPRYIMAASVISA"
BASE COUNT          284 a          149 c          147 g          196 t
ORIGIN      
        1 attataaact tgctacattg taaatataga caaataaaag gaattactat gccagcaaat
       61 atatcaggaa cacccttcaa ctcatttggt ataagcttta tccagaaaca gtcctgctgg
      121 aggaaatctg atgatatcct gcgatgttca atgggtcaga ggactatcaa gttatccact
      181 aatacgctga acaaccgaat tttgacctcc gtcgcccgac aaagcaccaa agatattaat
      241 gcctggaaaa gagatgaaca gacagtttac ccatctcgcg tgatcaacca gggaattgat
      301 aaatattgtg ctgaaaatag cagaaacatt tccagtgaag tacggcaaag agtttttaaa
      361 ttcattgaaa aggattacag tctcaagttg aatatcatag cagcgcaaag ctctatcaac
      421 catctcataa taggaaatgg ccgtttcggt gataaaataa atatgctttg caagggagtg
      481 agtagagaag taaagaacca gacaatggat gtaatagcca atcaactcgc agaccaattc
      541 ttccaaaagc atattagtcc agatgttgac atcaaacagc tacgagatga catccccaga
      601 tatattatgg ctgccagcgt catatcggca taaatagaga tacccttaac gctaaccaga
      661 aaaaaaatac ctataagagc ctgtacatag agttgtgtcg ttgcctgatg ttgatatgtc
      721 aaatccaaca tcaaatagcg gtaaatttat ggataaaaaa ttacaggctc tggtta
//
LOCUS       DAZFWP020000469          775 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFWP020000469 DAZFWP020000000
VERSION     DAZFWP020000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.478"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK0Z_RS27280"
                     /old_locus_tag="pgaptmp_005456"
     CDS             complement(<1..220)
                     /gene="ymfM"
                     /locus_tag="ACHK0Z_RS27280"
                     /old_locus_tag="pgaptmp_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor YmfM"
                     /protein_id="HGU4505246.1"
                     /translation="MNSLTTHYRRSQLIALPVPGGKAKVEYCYAVNVPGGRVIVTHSF
                     AEWAVGDFNRQKETVLCDKLTAGSKITTE"
     gene            complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK0Z_RS27285"
                     /old_locus_tag="pgaptmp_005457"
     CDS             complement(217..768)
                     /gene="ymfL"
                     /locus_tag="ACHK0Z_RS27285"
                     /old_locus_tag="pgaptmp_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4505247.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
BASE COUNT          183 a          222 c          169 g          201 t
ORIGIN      
        1 actccgtagt gatctttgaa ccagcggtta acttgtcgca aaggactgtc tccttctgcc
       61 ggttaaaatc acccacagcc cactctgcaa agctgtgggt tacaattacc ctgccacctg
      121 gtacattaac tgcatagcaa tactccacct tcgcttttcc acccggtaca ggaagcgcaa
      181 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacagtc
      241 acgacacgcc acggcgcccg gagctgcaca ctcgcgggcg tcattacttt ctgaaatgca
      301 aaaaattttg tagaccagtg ctgcatgctc ctgcagcttc gaaattgaga ggtacagctc
      361 gtcgttaatt gctgtcttct catgcggttc cactacaccg tcttcaattg ctgaacgaat
      421 ctgttttgaa taactgccga tctgttcaat gacctccagc aggcgttggt tgatatcggc
      481 gttgtccaca tcctcgacat caggaagaga cacaaagacg ccatttgcag actgcgccac
      541 agcgtcagca atgaagtgag ttccaccagc acgttgcaaa atcattgccc atcccagcgg
      601 gaaaatctga tcgccatcgg cacgaaggcg gttaaataat gcgttctctg ttacatccag
      661 ccagtcagca gcttcagcgt acccccccgg caacgctgcg atagtttttc tgacagcttt
      721 cacgtaccac tcaggctgtt tttctacttt ccagtgatgc ttacccacgg ttcac
//
LOCUS       DAZFWP020000470          765 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFWP020000470 DAZFWP020000000
VERSION     DAZFWP020000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.479"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(69..374)
                     /locus_tag="ACHK0Z_RS27290"
                     /old_locus_tag="pgaptmp_005458"
     CDS             complement(69..374)
                     /locus_tag="ACHK0Z_RS27290"
                     /old_locus_tag="pgaptmp_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4505248.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLEGWQRSAGAKAELALAEKLGHAVIFQEATSERN"
     gene            complement(371..652)
                     /locus_tag="ACHK0Z_RS27295"
                     /old_locus_tag="pgaptmp_005459"
     CDS             complement(371..652)
                     /locus_tag="ACHK0Z_RS27295"
                     /old_locus_tag="pgaptmp_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4505249.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            complement(685..>765)
                     /locus_tag="ACHK0Z_RS27300"
                     /old_locus_tag="pgaptmp_005460"
     CDS             complement(685..>765)
                     /locus_tag="ACHK0Z_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505250.1"
                     /translation="VCAALRELNKHRDILRDITATRELLR"
BASE COUNT          160 a          244 c          157 g          204 t
ORIGIN      
        1 acagtgctga ttttaattat tcagttctgg attatgcttt ctctttcacc tgccgtagtt
       61 cctggtaatt aatttcgctc actggttgcc tcctggaaaa taactgcatg ccccagtttc
      121 tccgccagtg ccagctctgc cttagcgcct gccgaccgct gccagccttc cagcatataa
      181 accgcatcca cacaacgaat cattgccatg caaatatcca tgtaatgtgg ctgagtcagc
      241 ccgtccggaa gtactgcggg atttaatacg gtatgccctt cctgtttcag tgcttcttcc
      301 gccttgtgaa acgcctcacg gttgaaattt tcatatcccg tcatcggacc ggcgatataa
      361 attctcaccc tcacgcctga accctcctgt cgaaataaac gtagttattc actgcgccca
      421 acttcatccc aaacttttcg gcaatttccc gtcggggtac gccacgctga tgcagttgcc
      481 gcgccagctc aatatcacgc tgtgaacatt tggctgactg gtgataatca ccccgtaaca
      541 tcatgcagat acccagttcc cgcgctttcg tcctgacggc agccccacta cggccaatca
      601 gactgccgat gctttcgact gtcatcgttc ccgcacactg ccggagtatc ataatttccg
      661 cccagcgcca cttcttccag ccactcaccg cagcagctct ctggtggcgg taatatcccg
      721 gagaatatcc ctgtgtttgt tcagttcccg cagcgcggcg cagac
//
LOCUS       DAZFWP020000471          763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFWP020000471 DAZFWP020000000
VERSION     DAZFWP020000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.480"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..510
                     /locus_tag="ACHK0Z_RS27305"
                     /old_locus_tag="pgaptmp_005461"
     CDS             85..510
                     /locus_tag="ACHK0Z_RS27305"
                     /old_locus_tag="pgaptmp_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4505251.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHK0Z_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHK0Z_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          220 a          178 c          177 g          188 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccttcac cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atccagctta
      661 gttagtacat aaatactatt ttgatgaaaa ttattatatg tttcctcaga ttttaatcag
      721 gcgcccagat taaaccaagt attgagcgcc tgattatgtt tat
//
LOCUS       DAZFWP020000472          760 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFWP020000472 DAZFWP020000000
VERSION     DAZFWP020000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.481"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(9..206)
                     /locus_tag="ACHK0Z_RS27315"
                     /old_locus_tag="pgaptmp_005463"
     CDS             complement(9..206)
                     /locus_tag="ACHK0Z_RS27315"
                     /old_locus_tag="pgaptmp_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4505252.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(448..>760)
                     /locus_tag="ACHK0Z_RS27320"
                     /old_locus_tag="pgaptmp_005464"
     CDS             complement(448..>760)
                     /locus_tag="ACHK0Z_RS27320"
                     /old_locus_tag="pgaptmp_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4505253.1"
                     /translation="KPELEAFFRDMTRGKQKSWLSHCTDTEALIIDRVISEVLGEYPG
                     LINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWLAVAEHMLYVPMHDSF
                     R"
BASE COUNT          201 a          201 c          147 g          211 t
ORIGIN      
        1 aaaagccttc aggactgaag gcgtctgtaa caaccgcact gatagtctgc cagacccgcc
       61 ataacaagct gggtcagtat taactggcag cgttcgcgtg aaaggtaagt attctgcgca
      121 acctccccga ctgtcgccgg ttcggtaacg cttaattcat taaacaccac tctggcggtt
      181 tcggtcatat cctgctgttt cagcatgtct ttttcccttt ttcggttaac gtgacacacc
      241 aataactctt gtcgaaaaag ccagcaagct gaaagaacgg tattaataac caccagcgaa
      301 tttattgcgc tgctgtatat tacggacaca aaaaaaccac cttccggtgg cttccttgtg
      361 cgaaaaaact tgcatttcgc ctcgcgatac agctttgcga agcttacagg aattcaagct
      421 gtttctgcgt aaaaaagcaa gcttttttta tcgaaatgaa tcgtgcatag gtacataaag
      481 catgtgttca gccacggcta accaacctgc aatacgtttc tcacatgtgc tgaaacacca
      541 ttccgggtga gtacgattta aacattctgc catttttctc ttactcattc cccgtccttc
      601 gtacctttgc cggagaatat tgattagccc gggatattcc ccaagcacct cactgataac
      661 gcgatcaata atcaacgcct ctgtgtctgt acaatgtgac aaccagctct tctgcttccc
      721 tctggtcata tcccgaaaaa atgcctcaag ttccggtttt
//
LOCUS       DAZFWP020000473          757 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFWP020000473 DAZFWP020000000
VERSION     DAZFWP020000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.482"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..669
                     /locus_tag="ACHK0Z_RS27325"
                     /old_locus_tag="pgaptmp_005465"
     CDS             <1..669
                     /locus_tag="ACHK0Z_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HGU4505254.1"
                     /translation="DARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQE
                     LNRTETAREVQRPSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGE
                     KTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALY
                     MPAMVATSKTEWGRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHN
                     PVAA"
BASE COUNT          184 a          189 c          246 g          138 t
ORIGIN      
        1 gatgcgcgca tgctggcaga gttctgtcgt cagaagcgcc ctgcagcctg ggaagcgcct
       61 cacccgcttg aacgcgcgtt gcgtgccctg gtagtccgcc accaggcgct gacagatatg
      121 cacacgcagg aactgaatcg cactgaaacg gcgcgggaag tccagagacc gagcattgat
      181 gctcaccttc tgtggcttga agcagagctg aagcgtcttg agaagcagat aaaagacctg
      241 acagacgatg atccggatat gaaacaccgc aggaaactgc tggaaagcat cccgggtatc
      301 ggagagaaaa catctgcggt attgctggct tatatcggtc tgaaggaccg cttcgcccat
      361 gccagacagt tcgccgcttt tgcgggtctg acaccacggc gttatgaatc aggtagcagt
      421 gtgagagggg cgagccggat gagtaaggcc ggacatgtgt cgcttcgcag ggcgttgtat
      481 atgcccgcaa tggtagccac cagtaagact gagtggggac gggcgttccg cgaccgtctg
      541 gcggctaatg gcaagaaagg aaaggtgatt ctcggcgcga tgatgcgcaa gctggcacag
      601 gtggcgtatg gagtgctgaa gtcaggcgtg ccgttcgatg cgtcacggca taatccggta
      661 gcggcgtaaa aatcgcggaa gggatgaaaa aaacagcgcc tgacggcgct gtgtctggca
      721 tgcctgcaat ccgggaaacc ggaccaggaa aaaactt
//
LOCUS       DAZFWP020000474          755 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFWP020000474 DAZFWP020000000
VERSION     DAZFWP020000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.483"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            156..722
                     /gene="slp"
                     /locus_tag="ACHK0Z_RS27330"
                     /old_locus_tag="pgaptmp_005466"
     CDS             156..722
                     /gene="slp"
                     /locus_tag="ACHK0Z_RS27330"
                     /old_locus_tag="pgaptmp_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU4505255.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          231 a          161 c          165 g          198 t
ORIGIN      
        1 ataccacgcc ccaaaataac ggaattgaac taaacagaag ggatataaac atcagacagg
       61 tttacgttac tatcaggcat atcacctcag aatcagatga aaactataaa gaaatatcta
      121 ttatggtttt aatatttgtt gataaggata gtaacatgaa catgacaaaa ggtgcactca
      181 tcctcagcct ttcatttttg cttgccgcat gtagttcaat tccgcaaaat atcaaaggca
      241 ataaccaacc tgatattcaa aaaagttttg ttgctgttca taaccagccg gggttatatg
      301 ttggtcaaca agcgcgcttt ggtgggaagg ttatcaacgt tatcaatggc aaaacggata
      361 cgttgttaga aatcgctgta ttaccgttgg atagctatgc gaagcctgat attgaagcca
      421 actatcaggg ccgactgctc gccagacaaa gcggcttcct tgatccagtg aactatcgta
      481 atcactttgt taccatcctc ggtaccattc agggtgaaca accaggcttt atcaataaag
      541 tcccgtataa cttcctggaa gtgaatatgc agggcatcca ggtgtggcat ttgagagaag
      601 tggttaatac cacctataac ctgtgggatt acggctatgg tgcattctgg ccggaaccgg
      661 gctggggtgc gccttactac accaatgcgg tgagtcaggt aacacctgag ctggtcaaat
      721 aacaccaccg aaagatgcag atgctttctc agcat
//
LOCUS       DAZFWP020000475          754 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFWP020000475 DAZFWP020000000
VERSION     DAZFWP020000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.484"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..83
                     /gene="yjbL"
                     /locus_tag="ACHK0Z_RS27335"
                     /old_locus_tag="pgaptmp_005467"
     CDS             <1..83
                     /gene="yjbL"
                     /locus_tag="ACHK0Z_RS27335"
                     /old_locus_tag="pgaptmp_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HGU4505256.1"
                     /translation="IIIAKIDLFIQRITHNLWHARKKMCF"
     gene            107..>754
                     /gene="yjbM"
                     /locus_tag="ACHK0Z_RS27340"
                     /old_locus_tag="pgaptmp_005468"
     CDS             107..>754
                     /gene="yjbM"
                     /locus_tag="ACHK0Z_RS27340"
                     /old_locus_tag="pgaptmp_005468"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGU4505257.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIK"
BASE COUNT          249 a          116 c          153 g          236 t
ORIGIN      
        1 atataattat tgcaaaaata gatttgttta ttcaacgaat cacgcataat ttatggcatg
       61 cacgtaaaaa aatgtgtttt taattgagca aataaatgat ctcaaaatgt gggtcaataa
      121 gtatattgac gattgcactg atgaggattt aaacgatcgt gactttattg catcagttgt
      181 tgaccgggct atttttcatt tcgcgattaa tagtatatgt aatcctgggg ataataaaga
      241 tgcgactccc attgaacaat gtacttttga tgtagaaact aagaatggcc ttccctccac
      301 ggttcagcta ttttatgagg aatctaagga taatgaacct ttagcgaata tacattttca
      361 agcaataggt tctggttttt taacgtttgt taatgcctgc caggaacatg atgacaacag
      421 cttaaaatta tttgcttcgc tgttaatttc actttcatat tctagtgcct acgcagattt
      481 atcagaaaca gtgtatatta atgaaaataa tgagagctac ctgaaagctc agtttgaaaa
      541 attatctcaa cgtgatatga agaagtacct gggagagatg aagcgtctgg ctgatggggg
      601 agaaatgaat tttgatggct atctggataa gatgtcacat ctggtgaatg aaggaacgct
      661 cgatcctgat attttaagca aaatgagaga tgctgcacca caattaatta gcttcgcgaa
      721 gtcgtttgac ccaacctcaa aggaagagat taaa
//
LOCUS       DAZFWP020000476          746 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFWP020000476 DAZFWP020000000
VERSION     DAZFWP020000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.485"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            32..>746
                     /locus_tag="ACHK0Z_RS27345"
                     /old_locus_tag="pgaptmp_005469"
     CDS             32..>746
                     /locus_tag="ACHK0Z_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4505258.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYV"
BASE COUNT          184 a          171 c          203 g          188 t
ORIGIN      
        1 cgggcagggg ataaattacc ggatttactc gatgaatata tcagagcaac aactgaataa
       61 tatgatggct gccgtttcgg ttgcgctgca gcctctggtc agggttgtac caatgacggc
      121 agttgaatgg gctgatcaaa attattatct gcctaaagaa tcttcatatg gtgagggaga
      181 atggaaaacg ctgccattcc agatcgccat tatgaactgt atgggtaacg accaggttcg
      241 cacggttaac ctgattaaat ctgcccgtgt tggctataca aagatgttgc tgggggtggt
      301 cgggtatttt attgagcata aatcccgaaa cagtctgctt tttcagccca cggattctgc
      361 cgctgaagat tttatgaagt ctcacgtgga ggcgacgatt cggaacgtgc catgcctgaa
      421 agacctttcc ccatggctgg gtcgtaaaca tcgtgacaat actctcacgc tgaaacgctt
      481 ttcatcgggc gtcggtttct ggtgcctggg cggcgctgcc gccaaaaact accgtgaaaa
      541 atccgtggac gtggtctgct atgacgaact ttcctcgttc gagccggatg tcgaaaaaga
      601 gggctcgcca accctgctgg gggataagcg tattgagggg tcggtgtggc caaaatccat
      661 tcgcggctcg acgcctaaaa tcaaaggcac ctgccagatc gaaaaagccg ctaacgagtc
      721 ggcgcatttt atgcgttttt atgtgc
//
LOCUS       DAZFWP020000477          745 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFWP020000477 DAZFWP020000000
VERSION     DAZFWP020000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.486"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..637
                     /locus_tag="ACHK0Z_RS27350"
                     /old_locus_tag="pgaptmp_005470"
     CDS             <1..637
                     /locus_tag="ACHK0Z_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGU4505259.1"
                     /translation="IEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT          237 a          109 c          161 g          238 t
ORIGIN      
        1 aattgaagat aatgttattg ctgaagaata ttcaacaatg cctgtgaaaa atttctccag
       61 tgtcggcgct ttttcatttc cgacctgcca tttttcaggg aatatacgta taggtcgatt
      121 ttgtagtatt gcatcaaatg taaaaataat gggtggaaat catcctttga atcgttttac
      181 tactcacatg atgacttata acggtgaatt tgataagttt gctatgagtg aatttgaacg
      241 tagctggaca ttgaaaccat tcattaccaa acctgaaaat ccgattatag gaaatgatgt
      301 ctggatagga aatgatgttg tattgaaagg tggcatcgca ataggtgatg gtgctgttat
      361 tgctgccaat tccgttgtta ccaaagatgt gcccccatat gccatagttg caggagttcc
      421 tgcaaaaata atcagattta ggtttgattc aaatgttatt gatgaactgt tgagaataaa
      481 atggtggaat tataactact cagatcttcc ggataataac aagtgtgatg atataaatta
      541 ttttgttgaa gagatgaatc gattgatatc agatggaaat attcaagaga gggactataa
      601 aaaattcaat ctttctgagg tattcagagg cctataatta tctgttttgc atgataaatg
      661 ggcaagataa ccagggcggg gtttgcaatt tcaatggggc agcgtcatca aggaggttgc
      721 aaacatcctg ccatgcatat acttt
//
LOCUS       DAZFWP020000478          742 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFWP020000478 DAZFWP020000000
VERSION     DAZFWP020000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.487"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>742)
                     /gene="wzx"
                     /locus_tag="ACHK0Z_RS27355"
                     /old_locus_tag="pgaptmp_005471"
     CDS             complement(<1..>742)
                     /gene="wzx"
                     /locus_tag="ACHK0Z_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099459345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O103 family O-antigen flippase"
                     /protein_id="HGU4505260.1"
                     /translation="NVLAISSSLLSMVGLVYFFGLKGALVSASLNNAVAGVWLITIII
                     KRPWFKFKYWVGHTPRHNITQMKNYFYMGVIGALTGPISMIVVRTILTNNFSLEDAGY
                     WQAVNRISEAYLAVLTTALTVYYFPKTAAARRYSEYITLLKTGACIVVPLALSMALTI
                     YGLKDFIISILFTADFIRARELFLFQNIGDFLRICSWLFATILLAKGYFKINAILEVM
                     FSIIFPVLVKFLIINFGLNAVSIAYSITY"
BASE COUNT          278 a          153 c          113 g          198 t
ORIGIN      
        1 ataagtgatg ctgtaggcaa tactaacggc atttagtcca aaattaatta tcaaaaactt
       61 taccaatact ggaaaaatta ttgaaaacat cacctccaat atagcattta ttttaaaata
      121 tccctttgct aacaatatag tggcaaacag ccaactacaa atccgcagaa aatccccgat
      181 attttgaaat aaaaaaagct cccgagcacg tataaagtca gccgtgaaca agatactaat
      241 tataaaatct ttcaagccgt agatggttaa agccatgctc aacgcgagcg gtacaacaat
      301 acaagccccg gtctttaaaa gagttatata ttcgctatat cttcttgcgg ctgcagtttt
      361 tggaaaataa taaactgtca atgccgtagt aagaacagcc agataagcct cagaaattct
      421 atttacagct tgccaatatc cggcatcctc taaagaaaaa ttattagtga gaatagttcg
      481 aacaactatc atcgaaatag gtccagttaa cgctccaatc acccccatat aaaagtaatt
      541 tttcatctga gtaatgttat gccgaggagt atgtcccacc cagtatttaa atttaaacca
      601 tggtctttta attataatag taataagcca gacacctgca accgcattat ttaatgatgc
      661 agacactaat gcgcctttga ggccaaaaaa ataaactaag cccaccatag ataacaacga
      721 tgaggatata gctaaaacat tc
//
LOCUS       DAZFWP020000479          739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFWP020000479 DAZFWP020000000
VERSION     DAZFWP020000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.488"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..458)
                     /locus_tag="ACHK0Z_RS27360"
                     /old_locus_tag="pgaptmp_005472"
     CDS             complement(<1..458)
                     /locus_tag="ACHK0Z_RS27360"
                     /old_locus_tag="pgaptmp_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HGU4505261.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGET"
BASE COUNT          208 a          181 c          185 g          165 t
ORIGIN      
        1 tcagtctcac cgtactggac acaaaactca cgggaaggat gcggctcgct gaacagcgga
       61 ctccattcgg tcaccccgtt ttcctgcaac atcgtgccga ggatgctttt gatgtcctcg
      121 ttctggaaga tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg gtgcaccttc
      181 atgctgtaca gcatctggtt tttgtcgttc tcccccagtt caaaccaggt caccacacct
      241 ttcacccggc gctgtacgtc atcgccctgc cataccgtca ggtaggccat tttgtccagc
      301 acctgcgcaa attcaaggga aagaaactgc tgactgacca gagaaaggtc gagggaaaaa
      361 agtgaagaga gtgactgggt gagatgaaag gaaaccaccg caaacgcgtc cggtggcagg
      421 ccgtccactt caagtgtgaa acgtaatccg gttgacatag caacctccgt gaaaatgaat
      481 aataatccgc acatattgca tgtacaacat gtcgggcgga tgagataagc gagtcgacat
      541 ctggcgttac cggggaatat cagaaaaatg tcatggttat gtgatgattt agtctgggat
      601 tttacttcag agaaacgggt gtctgtttag tcaacaaaat aatcacaaac gcaacggatg
      661 ggttctcgac gttattcacg acaggctcgt acattacagc gagtaataaa aaacctgata
      721 tgcaaagtca aaccaatat
//
LOCUS       DAZFWP020000480          738 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFWP020000480 DAZFWP020000000
VERSION     DAZFWP020000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.489"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(346..669)
                     /locus_tag="ACHK0Z_RS27365"
                     /old_locus_tag="pgaptmp_005473"
     CDS             complement(346..669)
                     /locus_tag="ACHK0Z_RS27365"
                     /old_locus_tag="pgaptmp_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4505262.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          190 a          139 c          179 g          230 t
ORIGIN      
        1 tgtgaatcag gctccggatt tgctgaagtg cagtttaata atgactgagg cataacctga
       61 ttcgtggtat gtgggtaaca agtgtaatct gtgtcacaat tcagtcagtt gacagttgcc
      121 tgtcagactg agcatttgtt aaaaaaattt cgcatggtga agccccctgt gcggaggggc
      181 gactggtgta taaggtgtca tctctgacga caacctggat cgagatacgc gggttcaggg
      241 acaccggact gaactcaccg ggaggcaccc ggcaccatgt tcatggtgat acagaaatgc
      301 ggcttcagcc cctctccgga ggggcttttt atctgaatga ttctgttatt ttcgcccgta
      361 tgggcatatt tccagaatgc agcgatgatt aaaacactta tctgtgatat ttcctgtgtt
      421 tgcagggcat tcctggctgc ttttaattaa attccagacg tttttattaa atggggtcac
      481 gttgtaaatg gtgacgggga gctctttatt attgagtatt acgcctgtcc gggttggtgt
      541 aaaaatacag cccggaggga ggaagatatc agactgatac cataatatta attttatgtt
      601 acaccatatg gctgaaaatg atatgtcaca tgctggctgg attaccgtat caatcactat
      661 ccagttcatt tgctttcctt attttcttaa ggttttaaat gatgccataa attataagtg
      721 atgttttgaa agcaatct
//
LOCUS       DAZFWP020000481          726 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFWP020000481 DAZFWP020000000
VERSION     DAZFWP020000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.490"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            19..>726
                     /locus_tag="ACHK0Z_RS27370"
                     /old_locus_tag="pgaptmp_005474"
     CDS             19..>726
                     /locus_tag="ACHK0Z_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HGU4505263.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHKQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNSLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDE"
BASE COUNT          283 a          133 c          135 g          175 t
ORIGIN      
        1 ttaagcggaa atgtaattat gagactgcca gagaaagttc tttttcctcc tgtcactagt
       61 ggcctgtcag ggcaggaaaa acaaaaaaaa ccgaagagca ttaccggatt tcaggaaaat
      121 tatcaacgca atatcaggcc aatcaaaaca gcatcagaag cccgactacg cttctttgat
      181 aaaatggttt cgaaagaaaa ctctctggaa gatgttgttt ctttaggtga aatgattcag
      241 aaggaaattt atgggcataa acaaagaaca ttttcaccag ttcatcatac aggtaactgg
      301 aaatcatcat tgttacacaa cgcgctcctt ggtctggcaa atgtttataa tggcttacgg
      361 gaaacagaat accctaacac tttcaacaga gatggtataa aaagtactaa ctcttttaga
      421 gataactcat tgacaaaaac aagaactccc agagataatt ttgaggaagg aataaaacat
      481 cctgaacatg caacaatacc atatgacaac gacaatgaaa gtaataaatt gctaaaagca
      541 ggaaagatag ctggtaacaa taacgagctg ttgatggaaa taaaaaagga atcccaaagc
      601 gaccatcaaa tccccctgtc agataagttc ctgaaaagga aaaaacgatc tcctgtagct
      661 gaagataaag ttcaaaactc gttaacacca gaaaattttg ttcagaaaat ttcacttagt
      721 gatgag
//
LOCUS       DAZFWP020000482          724 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFWP020000482 DAZFWP020000000
VERSION     DAZFWP020000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.491"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>724
                     /locus_tag="ACHK0Z_RS27375"
                     /old_locus_tag="pgaptmp_005475"
     CDS             <1..>724
                     /locus_tag="ACHK0Z_RS27375"
                     /old_locus_tag="pgaptmp_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HGU4505264.1"
                     /translation="SVDLIVTDPPYFKVKPEGWDNQWTGDEDYLKWLDQCLAQFWRVL
                     KPAGSLYLFCGHRLASDIEIMMRERFSVLNHIIWAKPSGRWNGCNKESLRAYFPATER
                     ILFAEHYQGPYRPKDAGYEAKGRALKQHVMAPLIAYFRDARAALGITAKQIVDATGKK
                     NMVSHWFSASQWQLPNESDYLKLQSLFARVAEEKHQRGELEKPHHQLLETYTSLNRQY
                     AELQSEYKHLRRYFGVTAQVPYT"
BASE COUNT          175 a          171 c          224 g          154 t
ORIGIN      
        1 attctgttga cctgatagtc acggacccgc cgtactttaa agtgaagcct gagggctggg
       61 ataaccagtg gacgggtgat gaggattacc tgaaatggct ggaccagtgt ctggcgcagt
      121 tctggcgggt gctgaaacct gccggaagtc tttacctgtt ctgtggccat cgcctggcat
      181 ctgacattga aatcatgatg cgtgaacgct tcagtgtgct gaaccatatt atctgggcga
      241 agccgtccgg acgctggaac gggtgcaaca aggaaagcct gagggcgtat ttccccgcca
      301 cagagcgcat tctgttcgcg gaacattatc aggggccgta tcgtccgaaa gatgccgggt
      361 atgaggcgaa gggcagggca ctgaaacagc atgtgatggc tccgctgatt gcttactttc
      421 gtgatgcgcg tgctgccctg gggataacgg caaaacagat agtggatgcc acaggaaaga
      481 aaaacatggt gtcgcactgg ttcagtgcca gtcagtggca gctaccgaac gaaagcgatt
      541 atctgaaatt acagtcgctg tttgcccggg tggcagaaga gaagcatcag cgcggtgaac
      601 tggaaaagcc ccaccaccag ctgctggaga cgtatacttc actgaaccgg cagtatgcgg
      661 aactgcagag tgaatataag catctgcggc ggtattttgg cgtgacggcg caggtgccgt
      721 acac
//
LOCUS       DAZFWP020000483          721 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFWP020000483 DAZFWP020000000
VERSION     DAZFWP020000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.492"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(10..669)
                     /locus_tag="ACHK0Z_RS27380"
                     /old_locus_tag="pgaptmp_005476"
     CDS             complement(10..669)
                     /locus_tag="ACHK0Z_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU4505265.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
BASE COUNT          208 a          210 c          166 g          137 t
ORIGIN      
        1 gcatacccat tacgcctgcc caatatacaa atagatcgcc agaaagtggc acacactacc
       61 gccgagcacg aagccgtgcc agatggcatg gttgtatgga atgcgtttgc agacgtagaa
      121 aatcaccccg agcgaataaa ccacgccgcc taccgccagt aaggtaacgc tgcccgccgc
      181 gagcttaact gccatttcat aaattaccac cagcgacagc cagcccatcg ccagataggt
      241 caccagagat aaaattttga atcggtgcgc gatggtcagt ttaaacagaa tccccagcaa
      301 tgccaggctc cagataacaa tcatcaaccc gcgcgctaac ggagaatcca gccccaccag
      361 caaaaacggc gtgtaggttc cggcaatcaa caggtaaata gcgcaatggt caaatttctt
      421 cagccacatt tttgcccgtt gatgaggaat ggcgtgatag agcgtcgaag cgaggaacag
      481 caggatcata ctgccgccat agaggctgta gctggttatc gctgtggcgc tggcattgag
      541 atccaccgcc tgaaccagta gcaacaccag cccaacgata ccaaacacca acccaatgcc
      601 gtgactgacg ctgttggcaa tttcctctgc cagcgaatat ccctgcttaa tgaggggctt
      661 ctgaaccata acttactccg gagaaacgta cacgcacatg tatacctctc cagcgtaact
      721 g
//
LOCUS       DAZFWP020000484          720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFWP020000484 DAZFWP020000000
VERSION     DAZFWP020000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.493"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            43..>720
                     /locus_tag="ACHK0Z_RS27385"
                     /old_locus_tag="pgaptmp_005477"
     CDS             43..>720
                     /locus_tag="ACHK0Z_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4505266.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDD"
BASE COUNT          236 a          125 c          161 g          198 t
ORIGIN      
        1 atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg taaatgggcc
       61 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      121 ggtgtagaaa tctatgctgc tgctaaacaa ggcgaacccg atccagaatt aaacacatct
      181 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      241 gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg ttatgaaggc
      301 tttggtccta atggctcaat gattatcgct gagacattga cttcaaatgt taaccgtacg
      361 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      421 gtcagctata tgtttgacaa tacgggtgtg attgtattta aagggacaga ccctgaccat
      481 atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga agaagaaggt
      541 aacattgtta tttatactga acctactgac cttcataaag gaatcgcggc tctaaaagca
      601 gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      661 ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
//
LOCUS       DAZFWP020000485          717 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFWP020000485 DAZFWP020000000
VERSION     DAZFWP020000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.494"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>717
                     /locus_tag="ACHK0Z_RS27390"
                     /old_locus_tag="pgaptmp_005478"
     CDS             <1..>717
                     /locus_tag="ACHK0Z_RS27390"
                     /old_locus_tag="pgaptmp_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HGU4505267.1"
                     /translation="DKTLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDI
                     NSLIIDYDNEDVQSYWEDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDA
                     VLEDQKDKNFIAKMIAEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKV
                     QDDHENDGDRIWQTLNDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAA
                     WLNEILKSDSPDIRCRNIDF"
BASE COUNT          259 a          102 c          140 g          216 t
ORIGIN      
        1 ctgataaaac acttaaacta ttacaagatt tgaaagactc cgatcattta aatatgattg
       61 taatatatac tagagagaat ttagaaacgg tttggatgca gatatcatcg actctcaaag
      121 gtgctctgga tatcaacagc ttgatcattg actacgataa tgaagatgtc caaagttatt
      181 gggaagacgt tgtattaccg aacttaaatg ataatggtaa taaagctctc acaagagatg
      241 aaacaatagc ctatattaaa gacagtaagc cttgtagaag aattaaaaga ttaatacatg
      301 atgatgctgt gttggaggat caaaaggata aaaacttcat tgcaaaaatg attgcagaat
      361 atgctgtgtc tagaaatgca attatttcta gcaacacatc tggcaatgtc attcggggtg
      421 atgaaagcgg agtaaaatgg attcaatgtg gtaatatctt tgtctcccta tttcataagg
      481 ttcaagatga tcatgaaaac gatggagata ggatttggca aactctcaat gattctctca
      541 ttgaatggaa accatcttat tatcagttaa taaaatctga aattcagaat gcaatcgaag
      601 ctgaggcttt atcttttgta aatcatttgg ctaacgatca ttacggtcaa gctgcgtggt
      661 taaatgagat attaaaatca gactcgcctg atattagatg tagaaatatt gactttg
//
LOCUS       DAZFWP020000486          714 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFWP020000486 DAZFWP020000000
VERSION     DAZFWP020000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.495"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>714)
                     /gene="tssI"
                     /locus_tag="ACHK0Z_RS27395"
                     /old_locus_tag="pgaptmp_005479"
     CDS             complement(<1..>714)
                     /gene="tssI"
                     /locus_tag="ACHK0Z_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4505268.1"
                     /translation="SLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSAQIRPS
                     SVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQMDGWR
                     NNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGT
                     ALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRY
                     NPADQDSSCWIRVAQAWAG"
BASE COUNT          109 a          232 c          209 g          164 t
ORIGIN      
        1 tgcctgccca cgcctgtgcc acacggatcc agcacgaact gtcttggtct gccgggttat
       61 aacggtccca gttgaacttc acccgcacgc gaccatgttc gtcgcagaag atttcctcgc
      121 ctgccggtcc tgtcacgaca gcgctctgcg ggccgtcgac cagcggtttc agcaacggct
      181 gtggtcgcca tgttctgtct gccgggatca ccgcaaaatg gttctccagc gccgtccccg
      241 ctccctgccg tcctggcacc gcctgtggct gttcgccgtg cagttcactt gccaccacct
      301 gccattcccg gttcaggttc gcctgcggat gccccgtcag cacaattcgc cgtcccggcc
      361 atatctccgg cgagcggctc attccccgcg cggtttctgc gttgtttcgc cagccgtcca
      421 tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgtccgggg tagtcataca
      481 cttcatactg cgtgcgctgg tagtcctggt gctggccttc ctgttcaaaa cgtccggccc
      541 agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagggcgg atttgtgcgc
      601 tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc ggattccagg
      661 ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg ctct
//
LOCUS       DAZFWP020000487          713 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFWP020000487 DAZFWP020000000
VERSION     DAZFWP020000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.496"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          234 a          166 c          131 g          182 t
ORIGIN      
        1 tgtcaacaaa aaaatcatat ttcttaaaaa tcattcaaaa tcaataaagt agaagaactt
       61 catctcttta gcaaacgcca ttaacaattg cctttccatt tagttaatcg ttttgttttc
      121 tttaagatta gtcctattag acaaatagca aaatggaata actacagcac taagacgttc
      181 cgctatatag ctattacctg ttcccctgac ataaggagca gatatacctc aaccccctat
      241 tccccagtgc tgaccgggag cggcggcggt ataaaaagga acaaggaaca aatggcagaa
      301 caagcaacca tgtttgtccc ccccgcataa ccgtaccatt cgcattaaga aatattccca
      361 tatctatccc gcaaaagagg acccgtcata catcaagtcc gttttactgg gcatcagatt
      421 atcgccgtaa ttagctcagt gaaagccggg taaacggtta gcaagctcag tggctcaaaa
      481 gtgcatagcg ctaagatggg cctggttaca cctgcccttt gctgcagatc gtgttgatcg
      541 gtgtgatgcc gccacacgtt taagtcaatc tggaaaactc acaatgcgct cttctataaa
      601 gaggactctt tgataaagta acccgaaaag ggaaagctaa ctgatatcaa tgtaccacct
      661 tagtaatcat cccttatata cagacaaaaa gaagttgcct gaaagccagg tat
//
LOCUS       DAZFWP020000488          710 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFWP020000488 DAZFWP020000000
VERSION     DAZFWP020000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.497"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..620)
                     /locus_tag="ACHK0Z_RS27400"
                     /old_locus_tag="pgaptmp_005480"
     CDS             complement(<1..620)
                     /locus_tag="ACHK0Z_RS27400"
                     /old_locus_tag="pgaptmp_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4505269.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          177 a          177 c          170 g          186 t
ORIGIN      
        1 ggcaccacgc cgatggtgtt ttcgtcacag gcttcaatca tgcgtttcgg gtccataaac
       61 aactgaccgg ggcgcatagg gatctcacgc agctccacat cccagtagcg ggcgaattta
      121 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgttgg tttgcctgca
      181 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      241 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggcgcagg cgcatgccac
      301 agatcggcaa ccatatttac gcaacgcagg tcgatggctg cggattgcgg atattcttct
      361 ttgtcgatcc agtttttatt gatcgacaaa tccatcaatt tatggacgtt ttcgtcgtcc
      421 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcatca
      481 ttgataatct ggaatgcgac atcatcgcgc atttcgtgca gcggaaatcg ttttgactcc
      541 gcgatagtag aaatggcctt tgcgccaaaa cgtgaatcga gtagttctga gcggaaatcc
      601 gttaacagct tctggtccat ttcgaactcc ttaaatttat ttgaaggcaa taaaaaagta
      661 ggatttatcc gcaatggaag caaggcatta caggcttaat ttaaataaca
//
LOCUS       DAZFWP020000489          709 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFWP020000489 DAZFWP020000000
VERSION     DAZFWP020000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.498"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            14..>709
                     /locus_tag="ACHK0Z_RS27405"
                     /old_locus_tag="pgaptmp_005481"
     CDS             14..>709
                     /locus_tag="ACHK0Z_RS27405"
                     /old_locus_tag="pgaptmp_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505270.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSIFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSR"
BASE COUNT          277 a          141 c          111 g          180 t
ORIGIN      
        1 aataaggaat gttatgtcaa ttgactttac cccaggtata ataaatacat atcacggcga
       61 tatttataac tgcacaacaa ataccgataa tgttaaaacg ccagacacac cgaagtggcc
      121 ttgtgataat tgggaagaac aacaacccat taattccata ttctctggag aaggatacaa
      181 ccctgaacaa tttgatttag cgcaacaaca actccaacaa attaatgcat gccacacgaa
      241 caccacatat acaaatgcag actactcaaa agttgtggtt caacttgtaa gtcttattaa
      301 taacattgaa acgataagtt cgacaccact tacgcaacag acacaatcaa tacttaatca
      361 gattaataac attagatatg aaaaaaacaa aagtgctgaa tgtcgaatca ttgttgtcgc
      421 taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg gaatacccat
      481 aacattcagt gtccaaacca tgttttcaga tactaatttt attgctgaac aacgagctga
      541 cttaccaact aatataaaag atattcaaag cctgtatcaa aaaatgacga agttatatat
      601 tgagcacagt gaaaataaaa acaggatgaa agtctttgcc ggaaccaact ttattgattt
      661 caatatgaca ggacagaacc tgtccgggtt cgttctaacc ttgtcaagg
//
LOCUS       DAZFWP020000490          708 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFWP020000490 DAZFWP020000000
VERSION     DAZFWP020000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.499"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..117
                     /locus_tag="ACHK0Z_RS27410"
                     /old_locus_tag="pgaptmp_005482"
     CDS             <1..117
                     /locus_tag="ACHK0Z_RS27410"
                     /old_locus_tag="pgaptmp_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4505271.1"
                     /translation="EPETQRVIYLREGYEHECFSPLEQFRRKFREIEVGHEH"
     gene            107..>708
                     /locus_tag="ACHK0Z_RS27415"
                     /old_locus_tag="pgaptmp_005483"
     CDS             107..>708
                     /locus_tag="ACHK0Z_RS27415"
                     /old_locus_tag="pgaptmp_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4505272.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWK"
BASE COUNT          190 a          169 c          190 g          159 t
ORIGIN      
        1 gagccggaaa cacaacgagt tatctacctc cgcgaaggct atgagcatga gtgcttcagc
       61 ccgctcgaac agtttcgtcg taaattcagg gaaatagagg tcggtcatga gcactaaatt
      121 aaccggctat gtatgggatg gttgcgctgc atcaggcatg aaattatcca gcgtggcaat
      181 tatggcccgc ctggctgatt tcagtaatga cgaaggtgtg tgctggccat caattgaaac
      241 tattgcccgt cagattggcg cggggatgag taccgtcagg acggctatcg cacggctgga
      301 agcagaaggc tggttaacgc gtaaggcgcg tcgccagggt aaccgcaatg cgtcgaatgt
      361 ttatcagctt aacgttgcga agcttcaggc agcggcattt tctcaactgt cagattctga
      421 cccgtcaaaa tctgacgcat caaaatctga cccgtcaaaa tttgatgcgt cgaaatctgg
      481 caaaaaagcg ggttttcacc cgtcagaatc tggcggggat ccgtcagtaa aatcaaaaca
      541 tgatccgtca gataaaaaac cttctcgtcc ggacgcttcg caaccggaca cgcagacgga
      601 tgaacaggat tttttaactc gccatcctga tgcggttgta ttcagcccta aaaagcgcca
      661 gtggggaacg caggatgatt tgacctgcgc acagtggctc tggaaaaa
//
LOCUS       DAZFWP020000491          706 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFWP020000491 DAZFWP020000000
VERSION     DAZFWP020000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.500"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>706
                     /locus_tag="ACHK0Z_RS27420"
                     /old_locus_tag="pgaptmp_005484"
     CDS             <1..>706
                     /locus_tag="ACHK0Z_RS27420"
                     /old_locus_tag="pgaptmp_005484"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4505273.1"
                     /translation="EDERVITGIASTPSPDRDGDILEPEGAEFGSTIPFLWQHDHSRP
                     VGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIKTGLVRGLSVGFRPHEY
                     TYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDRPFSAASGNRKPVVKIASSA
                     GASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMNKAAEEGRTLDVEEEEH
                     YDNTAAEIRQVDAHLKR"
BASE COUNT          157 a          185 c          226 g          138 t
ORIGIN      
        1 gaggatgaac gggtgatcac cgggattgcg tcaacgcctt ctccggatcg ggatggtgac
       61 atcctggagc cggagggggc tgagtttggc agtacgatcc cgtttctctg gcagcatgac
      121 cattcccgcc ctgtaggcca gtgtacggtg cgtcgggtca gggaagggct ggaaatcacg
      181 gcaatgctgg tgaagccgga gccggggatg ccctcccaga tggcagcccg gctggatgag
      241 gcctgggctg ccattaagac cgggctggtc agggggcttt ctgtgggctt ccggccccat
      301 gaatacactt atctggacgg aggcggactg cattttctgc gctgggagct gatggaggtg
      361 tctgccgtca ccgtgcccgc gaatgcggaa tgcaccatcc ggaccattaa atatttcgac
      421 cgcccgtttt ctgccgcgtc cggcaaccgg aaaccggtgg tgaaaatcgc atcttctgcc
      481 ggcgcttcgg cacagtcaat aacctctttt cataaggaaa agtcagcaat gaatactggt
      541 gaacagatta aaagttttga aaacaagcgt gcggcgctgg cagcctccct tgaggagatc
      601 atgaacaaag ccgcagagga aggccgcaca ctggatgtgg aagaggaaga gcactacgac
      661 aacaccgcag cggaaatccg tcaggtggat gcgcacctga agcgtc
//
LOCUS       DAZFWP020000492          706 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFWP020000492 DAZFWP020000000
VERSION     DAZFWP020000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.501"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..407)
                     /locus_tag="ACHK0Z_RS27425"
                     /old_locus_tag="pgaptmp_005485"
     CDS             complement(<1..407)
                     /locus_tag="ACHK0Z_RS27425"
                     /old_locus_tag="pgaptmp_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU4505274.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIA"
     gene            complement(404..>706)
                     /locus_tag="ACHK0Z_RS27430"
                     /old_locus_tag="pgaptmp_005486"
     CDS             complement(404..>706)
                     /locus_tag="ACHK0Z_RS27430"
                     /old_locus_tag="pgaptmp_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4505275.1"
                     /translation="PVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGAEMAQAE
                     IRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRLGGEK"
BASE COUNT          138 a          253 c          170 g          145 t
ORIGIN      
        1 gcaatcgcca cctgtgccgc ctgttcactg cgcacatcaa atgccgggcg cacaaacggg
       61 tgcggtggca tattcacggt ccccatttcc acaaaccgcc agtagaaagc attgcgcggg
      121 ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg gattaacccc ccggatatgc
      181 acaccggatt ccatcccgcc atcgcgggag caccgggaaa ggaccaccac attgcggcga
      241 agttttcccc tgcgtaccgg tgcccgtgac accacttctt ctttcagcac attcgcaccc
      301 gcacgggttg cctcacgcag cacccggtta ttttccgcac cactcagaag ctgcaaatcg
      361 cggctgatgt cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcccctccca
      421 gccgacagag aatttccaga cgcccgccgg tcgcatccgg cacgggcagc ccgacaacgt
      481 tcaggatccg gtcacgccat ggaccactca gcacatgaag tcgtgacgct gccgtgattt
      541 cccggccgga ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccgg
      601 actgcatccg ctcccggctg ctcctgccac ggatatccgc atgaattttc ccgcatgaca
      661 cccattcttc cgtcatttct ccggcagcat tacgggttaa caccgg
//
LOCUS       DAZFWP020000493          699 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFWP020000493 DAZFWP020000000
VERSION     DAZFWP020000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.502"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..608
                     /locus_tag="ACHK0Z_RS27435"
                     /old_locus_tag="pgaptmp_005487"
     CDS             <1..608
                     /locus_tag="ACHK0Z_RS27435"
                     /old_locus_tag="pgaptmp_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HGU4505276.1"
                     /translation="SPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQSN
                     FMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVIGK
                     ETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGGDPITRFNTQ
                     TRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          222 a          135 c          168 g          174 t
ORIGIN      
        1 ttagtccaat gacaatggta ggtagatcgt tagctcaggc ggctacacaa actcttagac
       61 ctgcggtcac cagagctgcg acacaagcgg gggtgggagc cagtggtatg aagttcgtgc
      121 ccatgcagtc gaactttatg attaatcatg gcaaactgac taaccagcta cttcaggccg
      181 tggccaaaca aacccgtagc ggtgatactc agcaatggtt caaacaggag caaattactt
      241 atatatccag aacagtaaac agagctattg atgactattg catgagtaat aattcggtaa
      301 ttggtaagga gaccaaaagc cacattttca aggctgttga aagcgcgata aagcagcctc
      361 tcgatatgaa ttgtgcacaa acgtcgattg gccatttctt acagtccaac aaatacttta
      421 ataaaaaagt tgatgagcag tgtggcaaag ggggggatcc aattacacgc tttaacaccc
      481 agaccaggat gatagaacaa gtctcacaag aaatctttga acagaaattt ggtggtttga
      541 aagttagtga tatcaaagct ctgactcaga gcacgattgc acagcatgta caggatactc
      601 ggttgtagga ataacctgtt atatatgcgt gttgggcata agttaagctt cctggtgtat
      661 agcgtagtct ttgctgatgt aagaaaaggc tatatacgg
//
LOCUS       DAZFWP020000494          693 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFWP020000494 DAZFWP020000000
VERSION     DAZFWP020000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.503"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..606
                     /gene="ydeO"
                     /locus_tag="ACHK0Z_RS27440"
                     /old_locus_tag="pgaptmp_005488"
     CDS             <1..606
                     /gene="ydeO"
                     /locus_tag="ACHK0Z_RS27440"
                     /old_locus_tag="pgaptmp_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU4505277.1"
                     /translation="FNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYL
                     NSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLK
                     DICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYAST
                     SYFIYAFRKHFGNSPKRVSKEYRCQSHMGMNTGNTMNALAI"
BASE COUNT          216 a          138 c          137 g          202 t
ORIGIN      
        1 tttaatgaaa ataccattaa aaaatatcta caatgcacta acatccagac agtgacgatg
       61 cctgttcctg cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg
      121 attgcttatc tcaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt
      181 tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg
      241 ctatccgttt ccgggaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag
      301 ctgaaagata tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag
      361 caagagcaaa cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat
      421 ttgatacgcg tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtaca
      481 tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag
      541 gagtaccgtt gtcaaagtca catgggtatg aatacgggca acacgatgaa tgctttagct
      601 atttgattat ttgctaacga gtagtcaacc acgcacccta cgtaaaaatt aaaaatgggg
      661 cagtcattcc ctgccccgcg ttgtttttag gtg
//
LOCUS       DAZFWP020000495          691 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFWP020000495 DAZFWP020000000
VERSION     DAZFWP020000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.504"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            11..643
                     /locus_tag="ACHK0Z_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /pseudo
     CDS             11..643
                     /locus_tag="ACHK0Z_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          238 a           97 c          148 g          208 t
ORIGIN      
        1 ggatgaatat atgggaaaaa ttaaaattgt ggtttcagat cagcagccgt ttatgattga
       61 tgggataatt ggatttctcg gacattatcc tgatttatat aaggttgttg ggggctataa
      121 agatctgaag aaagctatag cggagtgtaa caaatctaca gcctaaatat ttattttagg
      181 agaatttgca ggtggaatga tggggtccga actcgtaaaa tgggttaaat cgcataagat
      241 agatgctcat attattacat ttgtagcaaa gatgccgtat attgattcaa taaaattgct
      301 tgaagcaggt gcgaaaggat gcgtatggaa aaccagtcac ccggcgaaac taaatcgtgc
      361 tattgattcg attagtaatg gttacactta ttttgatagt gtacatatgg attgtgaaaa
      421 aatatcttcc aggtattcct ctgataatca acttacaaat cgtgagtcag aaatattaca
      481 actcatagct gatggtaaaa caaacaaaga aatcgctaat ttcttgcagt taagcagaaa
      541 aacagtcgaa actcatagac tcaatatcat gaagaaatta gatgttcaca gtggtatcga
      601 gttgatcaaa acagctttac gtatgggtgt ttgcactata taaaaaaaag aggctttagg
      661 ctcattttgc tgaagcctta cgattgtcta g
//
LOCUS       DAZFWP020000496          687 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFWP020000496 DAZFWP020000000
VERSION     DAZFWP020000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.505"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>687
                     /locus_tag="ACHK0Z_RS27450"
                     /old_locus_tag="pgaptmp_005490"
     CDS             <1..>687
                     /locus_tag="ACHK0Z_RS27450"
                     /old_locus_tag="pgaptmp_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4505278.1"
                     /translation="AVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQNALNSTTA
                     VGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSM
                     SLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMIN
                     HLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQQAAEQ
                     NKVYQSMLVM"
BASE COUNT          168 a          170 c          229 g          120 t
ORIGIN      
        1 ggccgtggcg gcagcccgcg ggacggagat gcagattgct gcagaggccc gtctggcggc
       61 cacacaggaa cgcctgaaca gaaatattgc tgccagaagc gccgcccaga atgcgctgaa
      121 cagtacaacg gcggtgggct cacgtctgat gagcggtgcg ctggggctgg ttggtggcgt
      181 acccggactg gtgatgctgg gggctgcagc atggtacacg ctgtaccaga atcaggagca
      241 ggccagggag tctgcgcgcc agtatgcact gacgatagat gaaatcgcgc ataaaacgcc
      301 gtcaatgtct ttgcctgaag cctcagataa tgaaggacga acacgggcgg cgctgacaga
      361 gcagaaccgg ctgattgatg aacaggccag tcgggtgaaa tccctgcagg aaaaaatcgc
      421 aggatatcag tatgttctgg cgaacccggg ctggacgacc ggtgacggat tcatgataaa
      481 ccatctgacc tcggtgaaga ccgtaacgga agggcttgct caggcaacag agcagcttgc
      541 cgttgagcag tcccgtctgg cacagatgca ggaaaaagcg cagtccattc aggatgtgct
      601 tgccgggctg gaagaccgtc gtgtggcgtt aattcgtcag caggcggcag agcagaataa
      661 ggtgtaccag tccatgctgg ttatgaa
//
LOCUS       DAZFWP020000497          674 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFWP020000497 DAZFWP020000000
VERSION     DAZFWP020000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.506"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..438)
                     /locus_tag="ACHK0Z_RS27455"
                     /old_locus_tag="pgaptmp_005491"
     CDS             complement(<1..438)
                     /locus_tag="ACHK0Z_RS27455"
                     /old_locus_tag="pgaptmp_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HGU4505279.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFAL"
     gene            complement(447..>674)
                     /locus_tag="ACHK0Z_RS27460"
                     /old_locus_tag="pgaptmp_005492"
     CDS             complement(447..>674)
                     /locus_tag="ACHK0Z_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4505280.1"
                     /translation="TIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTVKSALAF
                     LILIIYFAPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          230 a          127 c          117 g          200 t
ORIGIN      
        1 caatgcaaaa aaaacaatat aaagaattgc tttaacaaat tcttttacgg tttttaagcc
       61 aaatatacgc tttaagccat ttactggatt caatgaatca aacttaaatt taacagcttc
      121 agttgctaat tgcattttac tttgaaacca ggagacaagt attgttgtga acatgcaaac
      181 cagcaaaaaa ggaatgttcg ttttcaacca accaaccccc atagctttaa aatagtccca
      241 aatatctggt ttagcgttat gatcaaggat gtattcaaga atcgacataa tgtgatgcac
      301 atcaaaaaca tatccgagat ataaagtacc cacaagcatt attacagaga tggttaagtc
      361 tctacttttt agaatttgcc cttttttaga agcatcctgg agtttctttt gtgttggctt
      421 ttcagtttta ttggccatga aacactctat ttctcaatgt atagttgcaa ttgttctggg
      481 aaaaaggata acggcattac tcgttcagcc agtattgggg caaaataaat aattaatatt
      541 aaaaatgcca gcgcactttt gacagtcagt gaaatggcaa aagcatttag ttgtgaagca
      601 taacatgcca gcaatcccag gacagcctca ccgcccagca taacggcaat cacggggctt
      661 gcgtaaacga tagt
//
LOCUS       DAZFWP020000498          674 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFWP020000498 DAZFWP020000000
VERSION     DAZFWP020000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.507"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..609
                     /locus_tag="ACHK0Z_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /pseudo
     CDS             1..609
                     /locus_tag="ACHK0Z_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          244 a           98 c          151 g          181 t
ORIGIN      
        1 ggggcagcag tatgatgagg agtccggcct gtattacaac cgccaccgct attatgaccc
       61 gctgcagggg cggtatatca ctcaggatcc gattgggctg aaggggggat ggaacctgta
      121 tacatatccg ctgagcccgg tgaatagcat ggatccatta ggattatatg aatttaaatc
      181 aaaaaatata gatgatattg gaatatttgc attggcaatg tgtaatggag aatcaattaa
      241 cgagaataaa gaatatggtg gactaatatg taagaagcaa ggtgaatatt tacccatgaa
      301 tccgataagt tcaaatgata atgatagtgt agacttgcga aatataaaat gccctgaagg
      361 ttcagagaga gtaggcgatt atcacactca cggtttttac tctgacgata aaggaaataa
      421 agtaacaaaa gaaaatgatg tttatgatag tctaaatttt tcaagcaaag atttaacgaa
      481 ttcttatatg aatggaatgg aaaaaaagaa tacagtagtt acttgggaac accaaataac
      541 acctatctaa aatataatcc caaagctaaa gggaatggag ttacaattat caggcaaggg
      601 agtaattaaa atgaaggcaa tacaatgttg ttatttcctt gctttagtct ccagtcattt
      661 aatggctgga gtta
//
LOCUS       DAZFWP020000499          672 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFWP020000499 DAZFWP020000000
VERSION     DAZFWP020000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.508"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            105..>672
                     /locus_tag="ACHK0Z_RS27470"
                     /old_locus_tag="pgaptmp_005494"
     CDS             105..>672
                     /locus_tag="ACHK0Z_RS27470"
                     /old_locus_tag="pgaptmp_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4505281.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQFSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGS"
BASE COUNT          154 a          153 c          200 g          165 t
ORIGIN      
        1 atgaaaaaat ccactgcagg gatcagtatc agctgacggt ttattaaccc ggacgggcac
       61 ctcaggaggt gcctttttta ttgactgaaa acaaagaggt aatcatgcgg catttatacg
      121 caacgatatt attgtttact accctgctgg caggaattgc ctttcctgca caggctgaaa
      181 gcggacacgg tgcattttcc gtgggatatg ctcaggttca cccgggcggc gtaccggcat
      241 tgtccggtac cggtgcgcgt gcaggtgatt taaaagggat taacgtgaaa taccgttatg
      301 agttcacgga tcacctgggc ggcattgtcg cgctgagtta tgcatcggtg aagaaaagtg
      361 acacgatgaa gacgggtgaa aataccttcc attatgaaag cctgcgcggt cgttatgtca
      421 gtctgatggc cggccctgtc tggcagttca gtgagcgggt cagtctctat ggcatggccg
      481 ggatggcgta cacccgctgg tctgacagtg ttcaggatta ccggcgtgat gaagtgaaac
      541 cggggtatgt gaaggagacc accaccgcca gtgatggtca tactgcgcgt catctgtcgc
      601 cggcctggaa tgccgggatt cagttcagtc ccgtagagac ggtggttatt gaccttgctt
      661 atgaaggttc cg
//
LOCUS       DAZFWP020000500          672 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFWP020000500 DAZFWP020000000
VERSION     DAZFWP020000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.509"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..589)
                     /gene="sctD"
                     /locus_tag="ACHK0Z_RS27475"
                     /old_locus_tag="pgaptmp_005495"
     CDS             complement(<1..589)
                     /gene="sctD"
                     /locus_tag="ACHK0Z_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HGU4505282.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYL"
BASE COUNT          209 a          133 c          143 g          187 t
ORIGIN      
        1 tcaggtaatt aaaaaatgga gttaaatcag tggttgattc gcaacgtcca taaagcgcga
       61 catttttccc atgccatacc agagtgattg catgaagttt gttttctttt agctgctggt
      121 atacatcagc tctggtaaac gctgggggtt cagcttttgg ggaaagcaaa acgtaactgc
      181 ctatggcgcc agacaacgca atcgcagcac caatcacgct agccaggata agacgctttt
      241 tacgtcgatt tttggcagag aacctttctg gcacatcaga caatggcaag gtgtggtcaa
      301 tatcaccaat gatgaagcaa catcccgcta tatcgatgac ctttccgaca ggtaatgact
      361 gatctgcttc ataaggggtg ggctgtccat caatccaaaa ttcaaccgga gataaaagga
      421 aaacaccttc ctcacgtata tcaagtaaaa actgattgag gccttgttca aggggaaaat
      481 aaacaatatc gttatcttct gtgcctatcg ttaggtttcc catcggaagt tgtaattccc
      541 gattacgcat cgcaccattg agaagtttta ttttatatga ggataacatt tttaaatttt
      601 attcatcctg gtggttgaac aatgagaaaa aatatggtga acttacatca actaagaata
      661 gagtagaaag ga
//
LOCUS       DAZFWP020000501          670 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFWP020000501 DAZFWP020000000
VERSION     DAZFWP020000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.510"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(52..594)
                     /locus_tag="ACHK0Z_RS27480"
                     /old_locus_tag="pgaptmp_005496"
     CDS             complement(52..594)
                     /locus_tag="ACHK0Z_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4505283.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          204 a          139 c          118 g          209 t
ORIGIN      
        1 atggggatag cggccatgtt tattacctgg agaaacttac aattttattt attaatcctg
       61 cgccacacgc ataaccagag aagataacac atcacctgat tgcactgagt tatctgtcag
      121 tttcaggtat tttgctttaa acggaatagc atattgacct tccgctgttt tataactctc
      181 aatgttaaaa ggtgcagaaa catccaggac atcgctgtta tcacctaaat agagatagta
      241 acccacggta tcgttgctac tggctatcgc cttatcgtta ccggttattt tatcgttggt
      301 aatcaacgag agttttacac cagcaacttc tttcgggcag ctaattttta acatgctgct
      361 ggttacaccg actgcatcat cataggtaga atatttagct ttaccttcaa agacagtggt
      421 tggtacagtc ggcatattaa tacttactga atcaccaccc gcttcgaatt tacacggaga
      481 ttctgcaata ttccccttaa tagttaatgt tccggtatca acgtctgcag caaatactcc
      541 agcgctcata aaaaaagaag aaaagacagc ggcagcaata attgaacgtt tcataatatg
      601 ttccttaata aaatttattt ttggttgtta agagtctgta tctcttggaa acaaatttct
      661 cacatcaaaa
//
LOCUS       DAZFWP020000502          666 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFWP020000502 DAZFWP020000000
VERSION     DAZFWP020000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.511"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..384)
                     /gene="wzx"
                     /locus_tag="ACHK0Z_RS27485"
                     /old_locus_tag="pgaptmp_005497"
     CDS             complement(<1..384)
                     /gene="wzx"
                     /locus_tag="ACHK0Z_RS27485"
                     /old_locus_tag="pgaptmp_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099459345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O103 family O-antigen flippase"
                     /protein_id="HGU4505284.1"
                     /translation="MEKLNFAKVTILSGVVTCFRLICGLLISKVVAIYTGPIGIANLG
                     QLQNFVAFINGFISSQVSQGVNRYSAENQGDYENACFYWRASIKLSFIACCFIISTGV
                     LFSSKISEYLFYQSDLYWLIIIALCA"
     gene            complement(372..>666)
                     /locus_tag="ACHK0Z_RS27490"
                     /old_locus_tag="pgaptmp_005498"
     CDS             complement(372..>666)
                     /locus_tag="ACHK0Z_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGU4505285.1"
                     /translation="ISLPVIAGNEMSHVWHLFVIRTSERDSLQKHLKTEGITTLVHYP
                     IAPHNQEAYKEYSFLSLPLTERIHREVLSLPMDPTMSIDDINMVIEAINRWKS"
BASE COUNT          231 a          120 c          101 g          214 t
ORIGIN      
        1 agcgcataga gcaattataa ttaaccaata caaatcactt tgataaaata agtattctga
       61 tattttactg gaaaaaagca cccccgtact tataataaaa caacaggcta tgaaggataa
      121 tttaattgat gcacgccaat aaaaacatgc attttcgtaa tcaccttgat tttctgctga
      181 atatctatta actccttgtg aaacttgtga tgaaatgaat ccatttataa aggccacaaa
      241 attttgcaat tgtcccaaat ttgctattcc aattggacca gtataaattg caacaacttt
      301 tgaaataaga aggccacaaa ttagtctaaa gcatgttact acaccactga gtatcgtaac
      361 ttttgcgaaa tttagctttt ccatctatta atcgcttcaa ttaccatatt aatatcgtcg
      421 atgctcattg tcggatccat tggcagcgat aagacttctc tatgtattct ctcagttaaa
      481 gggagagata aaaaactata ttctttatat gcttcttgat tatgaggcgc tattgggtaa
      541 tgcactaatg tagtaatacc ttcagttttc aaatgttttt gcaaagaatc acgctctgaa
      601 gttctgataa caaaaagatg ccatacatga gacatttcat tgccagcaat tacaggcagt
      661 gaaatt
//
LOCUS       DAZFWP020000503          656 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFWP020000503 DAZFWP020000000
VERSION     DAZFWP020000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.512"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(17..656)
                     /locus_tag="ACHK0Z_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /pseudo
     CDS             complement(17..656)
                     /locus_tag="ACHK0Z_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          195 a          127 c          100 g          234 t
ORIGIN      
        1 tgactacgac agatgtttaa ttttttaaat tatcgtcttt attctcgatt tttgtgtctt
       61 caccctcaat gaatttatct tctgaagaga gctgttcatc ttgaacaggt tgcccagcat
      121 tctccacatc ttcaagccac agcagaagac gtaatatttc atctatattt tcgaagctaa
      181 cataatcgta gcgacgatgg gtagcataat tttttcgtgc taatttttta tctgtgataa
      241 ttggtattcc gatttccttt gcatatttcc ttacgcctaa ggccacctca ttagttactc
      301 ttacagaaat caatggaata ggtgaaagat gtggcttaaa gtatatccct attgcaatgt
      361 gagtagggtt ggaaatcatc aaacgactat tactgacatc agacttcaat tgctcagaaa
      421 gaatttcctg atgcgctctc tgcgtttaga cttaatttct ggatttcctt cttgctcctt
      481 gtattctctt ttaacttctt gtttatccat tttcatatct ttcataaata aaaaatattc
      541 agcgataaaa tcaaaaatta agacaattat catactgccg agacaataca gtatgagaag
      601 gaatagcatc tctccccaat ctgataataa agatatgata tctccatcaa gagttt
//
LOCUS       DAZFWP020000504          648 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFWP020000504 DAZFWP020000000
VERSION     DAZFWP020000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.513"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..291)
                     /gene="grlR"
                     /locus_tag="ACHK0Z_RS27500"
                     /old_locus_tag="pgaptmp_005500"
     CDS             complement(<1..291)
                     /gene="grlR"
                     /locus_tag="ACHK0Z_RS27500"
                     /old_locus_tag="pgaptmp_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HGU4505286.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEK"
     gene            488..>648
                     /locus_tag="ACHK0Z_RS27505"
                     /old_locus_tag="pgaptmp_005501"
     CDS             488..>648
                     /locus_tag="ACHK0Z_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HGU4505287.1"
                     /translation="MKKIILSIILISNSCYASDCFEITGKAYNIDPLILKAIAWNESK
                     NKNGIKSKI"
BASE COUNT          223 a          120 c           84 g          221 t
ORIGIN      
        1 tttttcattt cctcttacat ggcaatgtaa tgtgacatta ctatcattac tcagtacttt
       61 tttagggata actaattgat aatcttgttc aatgtttagc accgagggaa tttcatgatt
      121 atagcgatgg acatgaagta tgatgtcctc ctcttcagac aggaccccct gatacacaga
      181 agcaatatct ccgccagtga tcaaatttcc atttttaatc agtatacctt ccccacagga
      241 atcttcattg ctaataaaga taatgctata gatgccatcc ttcataatca taactcactc
      301 agtttcatac taacctcact cctttcaatt tgttctatcc ataagcgata tcaagaataa
      361 cggagacctt tctttttctc cagattgcaa tgtaatatat cattaaatat actcatattc
      421 caacctgttg ttttccgtgg ttgacatatt taatttctga tgaatcatat tgaatatcaa
      481 catgaaaatg aaaaaaatta tattgagtat cattctcatt tcaaattctt gttatgcttc
      541 tgattgtttt gaaattactg gaaaagcata taatatagac ccattaatat taaaagcaat
      601 tgcatggaac gaatcaaaaa acaaaaacgg aataaaaagt aaaataaa
//
LOCUS       DAZFWP020000505          645 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFWP020000505 DAZFWP020000000
VERSION     DAZFWP020000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.514"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            277..>645
                     /gene="nleB"
                     /locus_tag="ACHK0Z_RS27510"
                     /old_locus_tag="pgaptmp_005502"
     CDS             277..>645
                     /gene="nleB"
                     /locus_tag="ACHK0Z_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGU4505288.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVD"
BASE COUNT          234 a           99 c          114 g          198 t
ORIGIN      
        1 atcggtactt ttatcaaaaa acatatattc tattaattat tgccatcacc tttttcttat
       61 tgacaacatt actctgctga ttgttaaaat ctaacctgta ttcagggtta cgattctata
      121 caatagaaca gggaggggaa aggatgtcat cgttaatgag actttttgtt aatgtcaaca
      181 atgctatgaa tttattttag aaaaacaatt aagctgacgt agtgttccgt ctaaatgacg
      241 gggcgatatt aacatgatta gaacaagagg aattttatgt tatcttcatt aaatgtcctt
      301 caatccagct tcagaggaaa gacagcctta tcaaatagta cacttctcca gaaagtttct
      361 tttgctggaa aagaatatcc tctggaacct attgatgaaa aaacccctat tctttttcag
      421 tggtttgaag caaggccaga gcgatacgaa aaaggagaag taccaatatt gaataccaaa
      481 gaacatccgt atttgagcaa tattataaat gctgcaaaaa tagaaaatga gcgtataatc
      541 ggtgtgctgg tagatggaaa ttttacttat gaacaaaaaa aggaatttct cagtcttgaa
      601 aatgaatatc aaaatataaa aataatctac cgagcagatg tggat
//
LOCUS       DAZFWP020000506          644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFWP020000506 DAZFWP020000000
VERSION     DAZFWP020000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.515"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>644
                     /locus_tag="ACHK0Z_RS27515"
                     /old_locus_tag="pgaptmp_005503"
     CDS             <1..>644
                     /locus_tag="ACHK0Z_RS27515"
                     /old_locus_tag="pgaptmp_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505289.1"
                     /translation="PDRIQVSPLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEASAADGQYLAQAKWDT
                     PKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQ
                     GDPASVSFRIAAPAAPSQIELTPGYFQITAVPKLAVYDPTVQFEFWFSEAKIAD"
BASE COUNT          133 a          183 c          214 g          114 t
ORIGIN      
        1 gccggaccgg atacaggtca gccccctgcc tgatggtgtg gagacatacg gtgtatgggg
       61 actctccctg ccgtcactgc gtcgtcgcct gttccgctgt gtctccatcc gggaaaacac
      121 ggacggcacc tttgccatca cggcggtgca gcacgtaccg gaaaaggaag ccatcgtgga
      181 taacggggcc agctttgagc cgcagtcagg caccctgaac agcgttattc caccggcagt
      241 gcagcacctg acggtggagg cgagcgcggc tgacggtcag tatctggcac aggcgaaatg
      301 ggacacgccg aaggtggtga agggcgtgag ctttatgctt cgcctgaccg tggccgcgga
      361 tgacggcagt gagcggctgg tcagcacggc ccggacgacg gaaaccacat accgcttcac
      421 gcaactggcg ccggggaact acaggctgac agtccgggcg gtaaatgcgt gggggcagca
      481 gggcgatccg gcgtcggtat cgttccggat tgccgcaccg gcagcgccgt cacagattga
      541 gctgacaccg ggctattttc agataacggc ggtcccgaaa ctggctgtat atgacccgac
      601 ggtacagttt gaattctggt tttcggaggc aaaaatcgca gaca
//
LOCUS       DAZFWP020000507          641 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFWP020000507 DAZFWP020000000
VERSION     DAZFWP020000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.516"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>641
                     /locus_tag="ACHK0Z_RS27520"
                     /old_locus_tag="pgaptmp_005504"
     CDS             <1..>641
                     /locus_tag="ACHK0Z_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4505290.1"
                     /translation="FFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALN
                     GYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLD
                     ILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYI
                     NNNSEDKLIVFHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHEQLINTY"
BASE COUNT          215 a          105 c          127 g          194 t
ORIGIN      
        1 tttttttgtt tctcctggcg agtttcgtca gactggaacg gatgcatccc gcacaataaa
       61 tgaaaaacaa aagccggtaa ttatgttttt agttgtcggg gagacagcaa gaagtcaaaa
      121 ctatgctctg aatggatatt ctcgtggtac caatgatttt acaaagaaat ataatgaact
      181 tatatccttt cataatgtgc agtcgtgtgg cacatccaca gccatatctg ttccatgcat
      241 gttttcagat atgaagcgaa aagagttcaa ttcccgcaaa gcggtaaatt cggaaaatgt
      301 actggatatt ctgtacagaa cgggggtaaa tttactctgg attgaaaatg acgggggatg
      361 taaaggtgta tgtaagcgaa tccctacaat taatattgag ccatcaaatt cagataatac
      421 attatgcaag aaaaactcat gttatgatga ggttatgcta aaaaatattg atgagtatat
      481 aaataataac tcagaggata aacttatcgt atttcatttg atgggtagtc atggtcctac
      541 ctactatctc cgttatcctg agccacataa atattttaaa ccaacatgtg atcgtagtga
      601 tatcgaaaat tgtacgcatg agcaattgat taatacctat g
//
LOCUS       DAZFWP020000508          639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFWP020000508 DAZFWP020000000
VERSION     DAZFWP020000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.517"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>639
                     /locus_tag="ACHK0Z_RS27525"
                     /old_locus_tag="pgaptmp_005505"
     CDS             <1..>639
                     /locus_tag="ACHK0Z_RS27525"
                     /old_locus_tag="pgaptmp_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505291.1"
                     /translation="TSGEKIPLLSAANTWSAHQTFNGGITGALTGNADTATKLKTARN
                     INGVRFDGSADININTLVSRGRVTALGANAQGTSGIQLYEAYNNGYPSPYGNVLHLKG
                     ATAAGEGELFIGWSGTSGDHAPVHIRSRRDTDSANWSEWAQVYTSKDSVPGVNAKGNQ
                     DTSGNAATATKLQTARTINGVSFDGSKNIELTAEDLNLEQTVELAAGSLQKN"
BASE COUNT          171 a          153 c          173 g          142 t
ORIGIN      
        1 gtaccagcgg tgaaaagatc cccctgttga gtgcggctaa cacatggagt gcgcaccaga
       61 ctttcaacgg cgggatcacc ggtgcgctga cagggaacgc cgacaccgcg acgaaattaa
      121 aaacagccag aaacattaat ggagtcaggt tcgatggttc ggctgacatt aatatcaata
      181 ctctggtatc acgtggtcgc gtaacggcac tgggggcgaa tgcacagggg acatccggga
      241 ttcagctgta tgaggcatac aacaatggct acccttcccc ctatggcaat gtgcttcacc
      301 ttaaaggtgc caccgctgct ggcgaaggtg agttattcat tggctggagt ggcacgagcg
      361 gtgaccatgc gcccgtacat atccgttcgc ggcgtgatac tgattctgcc aactggtctg
      421 aatgggcaca ggtctatacg tcaaaagatt ccgttcccgg cgttaatgcc aaagggaatc
      481 aggatacctc tggtaatgcg gctacagcga ctaaattgca gacagcacgt actattaacg
      541 gtgtctcgtt tgatggctct aaaaatattg agttaacggc ggaagattta aatcttgagc
      601 aaaccgtaga attagccgct ggatcattgc agaaaaacc
//
LOCUS       DAZFWP020000509          637 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFWP020000509 DAZFWP020000000
VERSION     DAZFWP020000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.518"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..563
                     /gene="nleB"
                     /locus_tag="ACHK0Z_RS27530"
                     /old_locus_tag="pgaptmp_005506"
     CDS             <1..563
                     /gene="nleB"
                     /locus_tag="ACHK0Z_RS27530"
                     /old_locus_tag="pgaptmp_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGU4505292.1"
                     /translation="SYPASERDNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDF
                     FRNLAMLKAGTLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDC
                     NDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDY
                     NAFCDFIEFKHENIIPNTSMYTSSSW"
BASE COUNT          200 a           95 c          137 g          205 t
ORIGIN      
        1 aatcataccc tgccagtgag agggataatt atctgttagg cttattaaga gaagagttaa
       61 aaaatatccc agaaggtaag gactctttga ttgagtcata tgcagaaaaa agagaacata
      121 cttggtttga ttttttcagg aatttggcca tgttgaaggc tggaactttg tttacagaga
      181 cgggaaaaac tggatgccat aacatatcgc cctgtagcgg atgtatatat cttgatgccg
      241 acatgattat taccgataaa ttaggagtcc tgtatgctcc tgatggtatc gctgtgcatg
      301 tagattgtaa tgatgagata aaaagtcttg aaaatggtgc gatagttgtc aatcgtagta
      361 atcatccagc attacttgca ggcctcgata ttatgaagag taaagttgac gctcatccat
      421 attatgatgg tctaggaaag ggtatcaagc ggcattttaa ctattcatcg ttacacgatt
      481 ataatgcttt ttgtgatttt attgaattta agcatgaaaa tattataccg aataccagta
      541 tgtataccag cagttcatgg taagtcttcc ggttgtggtt gaaaaatatc gttctttagg
      601 tatagattca gatgattaat cctgttacta atactca
//
LOCUS       DAZFWP020000510          631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFWP020000510 DAZFWP020000000
VERSION     DAZFWP020000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.519"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>631
                     /locus_tag="ACHK0Z_RS27535"
                     /old_locus_tag="pgaptmp_005507"
     rRNA            <1..>631
                     /locus_tag="ACHK0Z_RS27535"
                     /old_locus_tag="pgaptmp_005507"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          145 a          140 c          212 g          134 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ctccttgaga gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg c
//
LOCUS       DAZFWP020000511          624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFWP020000511 DAZFWP020000000
VERSION     DAZFWP020000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.520"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(55..369)
                     /locus_tag="ACHK0Z_RS27540"
                     /old_locus_tag="pgaptmp_005508"
     CDS             complement(55..369)
                     /locus_tag="ACHK0Z_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4505293.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          179 a          163 c           92 g          190 t
ORIGIN      
        1 ccactaaagt cgctttttct tatggtaaca ggcaataacg ctctcagata ttttttagca
       61 tttttttgac cgcgcgtttc cggacgtatt ctgttctcct gtccctttat atcgtcggaa
      121 tacccgccgc tcttcaaatc ccattcccaa ctcagaatgt agtctgttga ccgcttgttt
      181 tatttcggtc aggttcaccg gtgaaaccgg agtccggcgc gccttacgca aacactctgc
      241 tcgtttctgt gccgccactt ttcttttctg gtcatcactt agctgtacca tcacttttgc
      301 ccatcgttca gctgctctcc ggtacagtcc ttttttctcc agacattctg ccacgtgatc
      361 atgtagcata agtgacctcc gattatctac agactgccat cctgaattta ccttccctta
      421 atgaaataac aataaaaaac aaaccacgca aaaacaataa aacaacacac aaaaaaaact
      481 aaataataaa caaaaataat caccttattt tattattttt tgagggagca attactgaac
      541 aaaaaacgct gactatatac tcaaaaccaa acaactattc tgccaatcag gtatcatggc
      601 aacacacgga attaccgtgt tttt
//
LOCUS       DAZFWP020000512          621 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFWP020000512 DAZFWP020000000
VERSION     DAZFWP020000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.521"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     repeat_region   27..543
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=27..55
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          154 a          176 c          160 g          131 t
ORIGIN      
        1 ttaattaata ataggttatg tttacagagt tccccgcgcc agcggggata aaccgccctc
       61 cagtactgtg atgcgatctt taagtccgag ttccccgcgc cagcggggat aaaccgactg
      121 gttctgtaac ccaaatttgg gatcattcga gttccccgcg ccagcgggga taaaccgact
      181 atcggcgctg ttcagttggg acagaaacag agttccccgc gccagcgggg ataaaccgtt
      241 atcgcaccgt gttaaaccgg cagaaaaaca gagttccccg cgccagcggg gataaaccgc
      301 gtgccttcga ctttgcggat cacgtcgtct tgagttcccc gcgccagcgg ggataaaccg
      361 acggggacga atcacagcag cgttatcgtg atgagttccc cgcgccagcg gggataaacc
      421 gaaaccggac gagggcgcgc taaaacgtga ttagagttcc ccgcgccagc ggggataaac
      481 cgcctctcaa tttgtaacgt ctgcaatgtc aggcgagttc cccgcgccag cggggataaa
      541 ccaccatata acccgttatc tctttctcaa gtttttatat tagcagtact tgtaataagc
      601 aacatatcca cgtaacacct c
//
LOCUS       DAZFWP020000513          621 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFWP020000513 DAZFWP020000000
VERSION     DAZFWP020000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.522"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>621
                     /locus_tag="ACHK0Z_RS27545"
                     /old_locus_tag="pgaptmp_005509"
     CDS             <1..>621
                     /locus_tag="ACHK0Z_RS27545"
                     /old_locus_tag="pgaptmp_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4505294.1"
                     /translation="AEGRKRSEEVNRLNHQTRVEKIINRSGIQPLHRKCTFGNYRVQN
                     DGQRHALSQAKSIAAELEGGCTNFVFSGRPGTGKNHLAAAIGNHLLAKGRSVIVITVA
                     DVMLALHGSYDNKNSGEKFLQGLCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASL
                     LSVGMLTNLNHAAMNTLLGERVMDRMSMNGGRWVTFNWESWRPNVS"
BASE COUNT          153 a          138 c          195 g          135 t
ORIGIN      
        1 ttgctgaagg gcgtaagcga agcgaggagg ttaatcgtct gaatcatcag acgcgggttg
       61 aaaaaatcat taaccgctcc ggtatccagc cgcttcaccg gaagtgtacg ttcggtaact
      121 accgggtgca gaacgatggt cagcgccatg ccctgagtca ggcgaaatcc attgcggcag
      181 agctggaagg cggctgtacg aattttgttt tcagtggcag gcctggcaca ggaaaaaacc
      241 acctggcggc ggctattggc aaccaccttc tggcgaaagg tcgcagtgtg attgtgataa
      301 cagtggcgga tgtgatgctg gcgttacatg gcagctacga caacaaaaac tcgggcgaaa
      361 aatttttgca gggattgtgt ggcgttgacc tgctggtact ggatgaaatt ggcatgcaac
      421 gggatacgcg taatgagcag gtcacgctga atcagattgt tgaccgcaga acggcatcgt
      481 tactcagtgt ggggatgctg acaaatctta accatgcagc gatgaataca cttctcggcg
      541 agcgggtgat ggaccgcatg tccatgaacg gtggtcgctg ggtgacgttt aactgggaga
      601 gctggcgtcc gaacgtcagc c
//
LOCUS       DAZFWP020000514          619 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFWP020000514 DAZFWP020000000
VERSION     DAZFWP020000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.523"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..339)
                     /locus_tag="ACHK0Z_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHK0Z_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          153 a          159 c          130 g          177 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgtt
//
LOCUS       DAZFWP020000515          616 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFWP020000515 DAZFWP020000000
VERSION     DAZFWP020000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.524"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>616
                     /locus_tag="ACHK0Z_RS27555"
                     /old_locus_tag="pgaptmp_005511"
     CDS             <1..>616
                     /locus_tag="ACHK0Z_RS27555"
                     /old_locus_tag="pgaptmp_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4505295.1"
                     /translation="TPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIRYR
                     YDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADG
                     SVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTS
                     ATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCAT"
BASE COUNT          154 a          178 c          191 g           93 t
ORIGIN      
        1 ggacgccacg cggcgaactg gcggtggtgt atgaccgtag tggcaaacag gtgcgcagct
       61 ttacttacga tgataaatac cggggccgga tggtggcgca ccgtcacacg ggccggccgg
      121 aaatccgtta ccgttacgac agcgacgggc gggtgacaga acagcttaac ccggcaggct
      181 taagctacac gtatcagtat gagaaagacc acatcaccat caccgacagc ctggaccgcc
      241 gtgaagtgct gcacacgcag ggcgaagccg ggctgaagcg ggtggtgaaa aaggaacacg
      301 cggacggcag cgtcacgcag agtcagtttg atgcggtggg caggcttaag gcacagacgg
      361 atgccgcagg ccggacaaca gagtacagcc cggatgtggt gacgggcctc atcacgcgca
      421 tcaccacgcc ggatggcagg gcatcggcgt tttactataa ccaccacagc cagttaacgt
      481 cagccaccgg gccagacggg ctggaaatgc gccgcaaata tgatgagtac ggacgtctga
      541 ttcaggaaac cgcccctgac ggcgatatca cccgctaccg ttatgataat ccacacagtg
      601 acctcccctg cgcaac
//
LOCUS       DAZFWP020000516          611 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFWP020000516 DAZFWP020000000
VERSION     DAZFWP020000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.525"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..587)
                     /locus_tag="ACHK0Z_RS27560"
                     /old_locus_tag="pgaptmp_005512"
     CDS             complement(<1..587)
                     /locus_tag="ACHK0Z_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4505296.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHNNTSLAW
                     GAGVQFNPIESVAIDIAYEGSGSGDWRTDGFIVGVG"
BASE COUNT          140 a          193 c          152 g          126 t
ORIGIN      
        1 ccgacaccca cgatgaaacc gtcagtgcgc cagtcgccac tgccggagcc ttcataagca
       61 atatcaatgg ccacggattc gatcgggtta aactgcacgc cagctcccca cgccagagac
      121 gtgttgttgt ggcgaccgtc atcacttccg gtcagcacat catgcgtttt ccccttgttg
      181 tcagttacgc ggagataatc cccggaaaaa gtcgacacac ggctgtaagc cacgcccgcc
      241 atcgcatacg cactgaacca ttcattcacg cgcacagagg gccccgccat cacgctgaac
      301 cagcggttac gcacggaatc ttcatgccag cgggtatcgc tgtaacgggt aagctggcga
      361 ttcctgtctc ctgcatagct gaatgacgtc accagcccca gtgtgtccgt aaactcataa
      421 cggtatttca cgttaatccc gttaagatta tcgctaccgg gagcgctcgt ccgggcatga
      481 agataccccg cgctcagcgt ggcctgctgc tcagacgccc atgcaggcgc gccggatacg
      541 gccagacaga tggctgcgga caaaatggcg gcataaagtt tacgcataat tacctctcgc
      601 ttttctgcaa t
//
LOCUS       DAZFWP020000517          607 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFWP020000517 DAZFWP020000000
VERSION     DAZFWP020000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.526"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            201..>607
                     /locus_tag="ACHK0Z_RS27565"
                     /old_locus_tag="pgaptmp_005513"
     CDS             201..>607
                     /locus_tag="ACHK0Z_RS27565"
                     /old_locus_tag="pgaptmp_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4505297.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHL"
BASE COUNT          168 a          123 c          158 g          158 t
ORIGIN      
        1 ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg gatccatgtc
       61 cggtttctct gcaagttaac tatatgaaaa atataaaaac aggtcttctg tgaaccggac
      121 atgaacaaaa aacagacatg taaaccggac atgaccggtt ttgttgtgat tgtgaggtga
      181 gagtttttgc gaggtgagga gtggctacgc agactgaagt tgccaggcat ttaagtctga
      241 ccgatcgcca gcttcgcaga ttgcagaaat tgccgggtgc cccgatatcg aataagcgag
      301 ggcaactgga tctggatgcc tggcgcgatt tttacatatc gtatctgagg agaagtaaaa
      361 acgatgtgcc tgatggcgat agcgaagacg actatgagga gaaattgctt attgccagat
      421 gggaactgac agcagaacag gctgttacac agcagttaaa aaatgaggtg tcaaaaggaa
      481 aacttattga caccgggttc tgtatttttg ccctcagtaa gctggcaatg gcgttatcca
      541 gtacgcttga ttccatccct ttatccatgc agcgacagtt tcctgattta acaccgcgcc
      601 atcttga
//
LOCUS       DAZFWP020000518          604 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFWP020000518 DAZFWP020000000
VERSION     DAZFWP020000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.527"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(116..191)
                     /locus_tag="ACHK0Z_RS27570"
                     /old_locus_tag="pgaptmp_005514"
     tRNA            complement(116..191)
                     /locus_tag="ACHK0Z_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Ile,seq:cat)
     gene            complement(220..>604)
                     /locus_tag="ACHK0Z_RS27575"
                     /old_locus_tag="pgaptmp_005515"
     CDS             complement(220..>604)
                     /locus_tag="ACHK0Z_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4505298.1"
                     /translation="ACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAI
                     ALQLHMSESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRS
                     YFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          174 a          161 c          121 g          148 t
ORIGIN      
        1 ctgagctaac aacgcagaat accgataatg gaccgccatc ggggacccgg ccccgcacca
       61 acaaccctgt tatggtgtcg tctgctcttc ctgataagct aatggcggtt tgtgatggtg
      121 gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc tcaccactga
      181 gctaaagggc cgagccaaaa aataataatc agatgaaaat caataatcaa gcccttgcct
      241 ggatacatat ctgtctggcg ggaagccata atagcggtga aatacagaaa taaagtagga
      301 ccggcttgaa taaccacatt tttctgctac agcctgtcca tatccatacc gggaacataa
      361 catatttaca gcaacacgca tccgctcttc cagcaacaag cgactgaaca tgcccccttc
      421 atttttcagt tttgtcttta acaaactctc actcatatgc aactgtagag caatcgcacc
      481 aagcgtccag cttgctgata tatctgtctg aattatcgcc ctgactttgg cacttatgct
      541 ggataaacat ccacttaaaa acaatgacat ccgttcatct gtttcaaaca gagacaggca
      601 ggcc
//
LOCUS       DAZFWP020000519          602 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFWP020000519 DAZFWP020000000
VERSION     DAZFWP020000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.528"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            331..>602
                     /locus_tag="ACHK0Z_RS27580"
                     /old_locus_tag="pgaptmp_005516"
     CDS             331..>602
                     /locus_tag="ACHK0Z_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGU4505299.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEG"
BASE COUNT          203 a          115 c          126 g          158 t
ORIGIN      
        1 cttaggttct aggtttataa aagaactgaa agaaagcagg tctttctctc gtgtagagat
       61 aagcgagtca gagttatggg gtatatcaaa taatcctgta aaccacgctc accaacgaaa
      121 cccaaacaaa aagtggtcat aacccgctca ggcggatttc attttcacgc aaacaacaga
      181 ataaacacag cgctgtgtat tcattccaac gagtgaatac acggagcaat gtcgctcgta
      241 actaaacagg agccgacttg ttctgattat tggaaatctt ctttgccctc caagatgagg
      301 gcgatttttt atctgtgagg atatgaacag atgtcaaaca tcaaaaaata catcattgat
      361 tacgactgga aagcatcaat agaaattgaa atcgaccatg acgtaatgac agaggaaaaa
      421 cttcaccaga ttaataattt ctggtcagac tctgaatacc gactcaataa acacggctct
      481 gtattaaatg ctgtattaat catgctggcg caacatgctc tgcttatagc aatttcaagc
      541 gacttaaatg catatggtgt tgtgtgtgag ttcgactgga atgatggaaa tggtcaggaa
      601 gg
//
LOCUS       DAZFWP020000520          601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFWP020000520 DAZFWP020000000
VERSION     DAZFWP020000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.529"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..154)
                     /locus_tag="ACHK0Z_RS27585"
                     /old_locus_tag="pgaptmp_005517"
     CDS             complement(<1..154)
                     /locus_tag="ACHK0Z_RS27585"
                     /old_locus_tag="pgaptmp_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4505300.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPG"
     gene            complement(415..>601)
                     /locus_tag="ACHK0Z_RS27590"
                     /old_locus_tag="pgaptmp_005518"
     CDS             complement(415..>601)
                     /locus_tag="ACHK0Z_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4505301.1"
                     /translation="QLRLGPADILESDENGIIPEQARVITQVVILDADKKQIQCVVRP
                     LQILRADGRWENIGGMK"
BASE COUNT          153 a          156 c          136 g          156 t
ORIGIN      
        1 taccgggctg agaggcggtg taagtgaact gtagttgcca tgttttacgg caaggagagc
       61 agagatagcg ctgatgtccg gcagtgcttt tgccgttacg caccacgcct tcagtagcgg
      121 agcaggaagg acatctgatg gaaatggaag ccacgcaagc accttaaaat caccatcata
      181 cactaaatca gtaagttggc agcattaccg agattgtttt gtcggaactg atacgctggc
      241 gctaataggt agtagggttc ttatgctaaa catacaacct ctttcttaat tctcgaacct
      301 gtttaggatt ctgtgtaaat tcaaaataaa cctgtctgaa cattcctaac aaacatccac
      361 cggacatgac aacaaaaaac cggagccgga ctccggtttt tgtgaagctg tcggctattt
      421 cattccgcca atattttccc acctcccgtc agcacgcagg atttgcagcg gtcttaccac
      481 gcactgtatc tgctttttat ccgcatccag tatcaccacc tgcgtgatta ccctggcctg
      541 ctccgggata atgccattct catcggactc cagaatgtct gccggtccca gtcgcagctg
      601 t
//
LOCUS       DAZFWP020000521          601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFWP020000521 DAZFWP020000000
VERSION     DAZFWP020000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.530"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(2..>601)
                     /gene="nleE"
                     /locus_tag="ACHK0Z_RS27595"
                     /old_locus_tag="pgaptmp_005519"
     CDS             complement(2..>601)
                     /gene="nleE"
                     /locus_tag="ACHK0Z_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HGU4505302.1"
                     /translation="IYPEIKHDYFNESPNIYDKKYISGITRGVAELKQEEFVNEKARR
                     FSYMKTMYSVCPEAFEPISRNEASTPEGSWLTVISGKRPMGQFSVDSLYNPDLHALCE
                     LPDICCKIFPKENNDFLYIVVVYRNDSPLGEQRANRFIELYNIKRDIMQELNYELPEL
                     KAVKSEMIIAREMGEIFSYMPGEIDSYMKYINNKLSKIE"
BASE COUNT          172 a          117 c           87 g          225 t
ORIGIN      
        1 tctactcaat tttagaaagt ttattattta tgtatttcat ataactgtct atttccccag
       61 gcatgtagct aaagatttct cccatttcac gtgcgataat catttcagat tttactgcct
      121 ttaactctgg taactcataa tttaattcct gcatgatatc tctttttata ttatataatt
      181 ctataaatct atttgcccgt tgttctccta gagggctgtc atttctgtac acaacaacta
      241 tgtataaaaa atcattattt tctttaggga agatcttaca acaaatgtcc ggaagctcac
      301 ataatgcatg taaatcagga ttgtataaac tatctacaga aaactgcccc attgggcgtt
      361 ttccggatat aactgttagc cagcttcctt ccggtgtact ggcttcattt ctggaaatag
      421 gttcaaacgc ttctggacat acagaataca tagtcttcat ataagaaaac cgtctggctt
      481 tctcgttaac aaattcttcc tgttttagtt cagctactcc tctggttata ccggatatat
      541 acttcttatc atatatattg ggtgattcat taaagtaatc atgtttaatt tccgggtaaa
      601 t
//
LOCUS       DAZFWP020000522          598 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFWP020000522 DAZFWP020000000
VERSION     DAZFWP020000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.531"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..299)
                     /locus_tag="ACHK0Z_RS27600"
                     /old_locus_tag="pgaptmp_005520"
     CDS             complement(<1..299)
                     /locus_tag="ACHK0Z_RS27600"
                     /old_locus_tag="pgaptmp_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HGU4505303.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNA"
     gene            complement(296..>598)
                     /locus_tag="ACHK0Z_RS27605"
                     /old_locus_tag="pgaptmp_005521"
     CDS             complement(296..>598)
                     /locus_tag="ACHK0Z_RS27605"
                     /old_locus_tag="pgaptmp_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4505304.1"
                     /translation="PVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGAEMAQAE
                     IRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRLGGEK"
BASE COUNT          114 a          213 c          144 g          127 t
ORIGIN      
        1 gcattgcgcg ggttatccgc cttcatggtg ttatcgctgt taccggtgtc cggattaaca
       61 ccccggatat gcacaccgga ttccatcccg ccatcgcggg agcgccggga aaggaccacc
      121 acattgcggc gcagttttcc cctgcgtacc ggtgcccgtg acaccacttc ttctttcagc
      181 acattcgcac ccgcacgggt tgcctcacgc agcacccggt tattttctgc accactcaga
      241 agctgcaaat cgcggctgat gtcctccagc cccgaaaaat ccagcagggt ttcgatcatt
      301 tttcacctcc cagccgacag agaatttcca gacgtccgcc ggtcgcatcc ggcacgggca
      361 gcccgacaac gttcaggatc cggtcacgcc agggaccact cagcacatga agtcgtgacg
      421 ctgccgtgat ttcccgaccg gactgaccgc gcacccagat gcggatttcc gcctgcgcca
      481 tttccgcacc agactgcatc cgctcccggc tgctcctgcc acggatatcc gcatgaattt
      541 tcccgcatga cacccattct tccgtcattt ctccggcagc attacgggtt aacaccgg
//
LOCUS       DAZFWP020000523          591 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFWP020000523 DAZFWP020000000
VERSION     DAZFWP020000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.532"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..410
                     /locus_tag="ACHK0Z_RS27610"
                     /old_locus_tag="pgaptmp_005522"
     CDS             <1..410
                     /locus_tag="ACHK0Z_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505305.1"
                     /translation="PSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMILSRVYDFLLP
                     AQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETISERLIREADT
                     REYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            510..591
                     /locus_tag="ACHK0Z_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /pseudo
     CDS             510..591
                     /locus_tag="ACHK0Z_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          188 a          105 c          103 g          195 t
ORIGIN      
        1 caccttcatt aagaaagaaa aattttactg acgcatataa tgcagcatct tctcatatcg
       61 aatactggat aggtgctggc gtctcattta tctcgatgat gattttatcc agagtatatg
      121 actttctttt acccgcccaa gatactttcc cgggtgatat catcagaagt ttatgtgttg
      181 tgtgtccatc catattaatc tggtttcagt tttcggtata tgtcatgagg aaatattaca
      241 gacatattct ggtgcgagga aaagaaaccg aaaccatttc agaaaggttg atacgtgagg
      301 ctgatacacg agaatatgaa ctatggcgtc cggtccgtcg ctttttctcc attgttttcc
      361 tcattgttct attaggttgc attcattcac tgataacaac tataaaataa aagttatggc
      421 tgttctgata caggaaaata aacgttcatt ctatggttac ctcttcctgt tatatagaag
      481 aattttaaac gacaagtaag ggaatataaa tgaaaataac aaaaaataaa aatgcaacta
      541 taaaaaacag tcatcagtcg gctgcttttc aatgtttacc ttatcatttt c
//
LOCUS       DAZFWP020000524          590 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFWP020000524 DAZFWP020000000
VERSION     DAZFWP020000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.533"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>590
                     /locus_tag="ACHK0Z_RS27620"
                     /old_locus_tag="pgaptmp_005524"
     CDS             <1..>590
                     /locus_tag="ACHK0Z_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4505306.1"
                     /translation="NKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTR
                     DSVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCP
                     DCGAVMGDATYQETFDEEYQVEVQEDDPEKMEGAEHPHKENTGGNQHHDSDNETGETA
                     DHSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAP"
BASE COUNT          197 a          143 c          155 g           95 t
ORIGIN      
        1 aacaaaatta tgcaggcagc caatatcagc cagcctgacg ccgataagtt gcttgctgcc
       61 tctcgcggag aatttgttgc agggattagc gacccgaatg atccgaaatg ggttaagggg
      121 atccagaccc gcgattctgt aaaccagaac cagcatgaat cggaacggaa ctaccaaaaa
      181 gcggaacaaa acagcccaaa tgcgttacaa aacgagccag aaacgaaaca gcctgaacca
      241 gtggcgcaac aggaagtgga aaaagtctgc accgcctgcg gtcagaccgg cggcggcaac
      301 tgccctgatt gtggcgcagt gatgggcgac gcaacatacc aggaaacatt cgatgaagag
      361 tatcaggttg aagttcagga agatgatccg gagaaaatgg aaggcgctga acatccacac
      421 aaggagaaca ctggcggcaa tcagcatcac gatagcgata atgaaactgg cgagacggca
      481 gatcactcaa ttaaggtgaa cggtcatcaa gaaatcacat ccaccagcag gacgtgtgac
      541 catctaatga tcgaccttga aaccatggga aaaaatcctg atgccccgat
//
LOCUS       DAZFWP020000525          583 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFWP020000525 DAZFWP020000000
VERSION     DAZFWP020000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.534"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..583
                     /locus_tag="ACHK0Z_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /pseudo
     CDS             1..583
                     /locus_tag="ACHK0Z_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
BASE COUNT          177 a          107 c          128 g          171 t
ORIGIN      
        1 cgtgccgata gtcgctttga acttattcag aaaaacgttg gctatttctt taaagatatt
       61 gaacgttcgg ccctgacatt aaaggactca ctgtatttat taaaaaatac agaggagatt
      121 caacgcgccg tgattctgaa aatggaaatg atgccatttt tagactcggt gggactggta
      181 cttgatgata ataaatatta tctcttttcg cggagggcga atgataaaat cgttgtttat
      241 catcaggaac aagtaaatgg accgcttgtc gacgagtcag ggcgggttat ttttgccgat
      301 tttaacccat cgaaacgacc gtggtcggtg gcttcagatg actctaacaa cagctggaat
      361 ccggcataca attgctttga tcgtccgggt aaaaaatgta tctcttttac gctacgtatc
      421 aacggcaaag atcacgattt gttagcggta gataaaattc atgtcgattt aaactgacgg
      481 tatctgaacg agtatcttga tcaaatcagc gctaatgatg aagttctatt tttgaaacaa
      541 ggtcatgaga tcattgccaa gaatcaactc gctcgtgaaa aac
//
LOCUS       DAZFWP020000526          578 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFWP020000526 DAZFWP020000000
VERSION     DAZFWP020000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.535"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..327
                     /locus_tag="ACHK0Z_RS27630"
                     /old_locus_tag="pgaptmp_005526"
     CDS             <1..327
                     /locus_tag="ACHK0Z_RS27630"
                     /old_locus_tag="pgaptmp_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
                     /protein_id="HGU4505307.1"
                     /translation="FYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGW
                     PLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYL
                     NIKHYQ"
     gene            344..578
                     /locus_tag="ACHK0Z_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /pseudo
     CDS             344..578
                     /locus_tag="ACHK0Z_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
BASE COUNT          150 a          123 c          156 g          149 t
ORIGIN      
        1 ttttacgcgt atgacaggat acggaggacg gttgtggcgc acgtattcgg tgaacgcact
       61 ctggccacac tggagcgtct tctgagcctg ctgtcggcct ttgaggtcgt ggtatggatg
      121 acggatggct ggccgctgta tgaatcacgc ctgaagggaa agctgcacgt tatcagcaag
      181 cgttacactc agcgcattga gcgacataat ctgaatctga gacaacatct ggcaaggctg
      241 ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat
      301 tatctgaaca taaaacacta tcagtaagtt ggagtcatta cctgagaata atacctcctg
      361 ggtaagtgag cagatacttt cagcgcctcc gaaagatgta aatattcgac ttggagtcgg
      421 ttctttagaa ggtacaaccg tctcacatgt tcagcgattg catcattcgt taatcgcagc
      481 tggtttggaa agtaacctca ctgtctatac cggtggtcac gattatgcct ggtggcgcgg
      541 agcaattatt gatgaattag caaattataa ttgcagga
//
LOCUS       DAZFWP020000527          577 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFWP020000527 DAZFWP020000000
VERSION     DAZFWP020000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.536"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..577
                     /locus_tag="ACHK0Z_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /pseudo
     CDS             2..577
                     /locus_tag="ACHK0Z_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          147 a          142 c          121 g          167 t
ORIGIN      
        1 tatttctgat ttcactccct ctgattattt ttaccaaccc tgggtcactg cattatcgcc
       61 aacgccccga cacgtcagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac
      121 tgtgcatcca cgtaatgccg acaatattga taatctgaat aggtccgtag agtatataaa
      181 agagttcttt gtcagtagcg gtgccagagt taccgcgcag ggcgtccccg attggctatt
      241 acgactctgc gcctggtagc caggattacc cttacccgca atgtcctggc tttatcccga
      301 tcggggggac tttattgcct ggtcggcaga atacaggata tcaacgccgt tcgtcaggta
      361 aaaacggcat tgttatcatc ccgggattta tctgtttatt ctatgaatgc cccatggttt
      421 attcccggta ttgatttctc tgaccacctg aattattggc aacacgatat tcccgccgta
      481 atgattactg acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgatacagca
      541 gacagattga attatcagaa aatggctcag gtggtgg
//
LOCUS       DAZFWP020000528          576 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFWP020000528 DAZFWP020000000
VERSION     DAZFWP020000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.537"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            245..>576
                     /locus_tag="ACHK0Z_RS27645"
                     /old_locus_tag="pgaptmp_005529"
     CDS             245..>576
                     /locus_tag="ACHK0Z_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505308.1"
                     /translation="MQLLSLRTIDGDTLYLKFRNVITEAFLDTPTKSYDFFGDVVLSQ
                     CINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILI
                     IIRNQMDVG"
BASE COUNT          188 a           85 c          119 g          184 t
ORIGIN      
        1 tggatagata aagaagcaac aattgaggag gcatatcgtg gaacagatga agcatactat
       61 tctgttataa aaccttgcgc tgaaaaactg gctaaagatc ctgataacat acaaatacta
      121 atggagggaa taggaagata gtatgcgcat tatgttaata ttactttgtt tttttctttc
      181 ccctgttttt caggcgataa gattaaaatc gtgaaaacct gtcaactgaa aaaaaaatga
      241 aagtgtgcag ttgctttccc ttagaaccat tgacggtgat acattatatc taaaattcag
      301 aaatgttatc accgaggcct ttttagatac tccaactaaa agttatgatt tttttggaga
      361 tgtggttctt tctcagtgca taaatggttc tcttatcttt gcgctggaat atggttctcc
      421 atatataaaa ggttgtctgg taactggttg ggaaaatggg tttaaaggta agaactctcc
      481 tgaaggcttt tgtttcgcag agagaaatat tcccgaagcg gtatggtttg gaaaaaacaa
      541 tattttaatt atcatccgaa accaaatgga tgttgg
//
LOCUS       DAZFWP020000529          575 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFWP020000529 DAZFWP020000000
VERSION     DAZFWP020000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.538"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..575
                     /locus_tag="ACHK0Z_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /pseudo
     CDS             1..575
                     /locus_tag="ACHK0Z_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Non-LEE-encoded type III effector H"
BASE COUNT          177 a           93 c          150 g          155 t
ORIGIN      
        1 gcctgataat cgtgttttat ccctctgtaa gggatgctgc cgctcactct gataatgggg
       61 cgcaagtaaa ggttagcaac agaacatatc gcgttgtttt aagtgataat aggttttgcg
      121 tgacaagaga gagtcatagt ggatgtttta ctaatatgct gtatagactg ggatggccta
      181 agggagagat caccagaaaa attgaggcta tgctgaattc atcgacagtg agcacgacta
      241 tagaaagagg atctgttcgt tcgaacagac ctgatttacc tccagtggat tatgcgcagc
      301 cggagttacc gccagcggat tatactcagt cagcgttgct gaggcttagc aacaacaaat
      361 cacccgtgct aggtaacgtt attggtaaag gtggtaatgc tgtcgtgtat gaagatatgg
      421 aaaatacaac aaaagtgttg aagatgttta ctatatctca aagccatgaa gaggtgacaa
      481 gcgaagttcg ttgtttcaac cagtattatg gtgccgggag tgcagagaaa atatatggcg
      541 ataatagaga tattattggt attagaatgg ataaa
//
LOCUS       DAZFWP020000530          568 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFWP020000530 DAZFWP020000000
VERSION     DAZFWP020000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.539"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>568)
                     /locus_tag="ACHK0Z_RS27655"
                     /old_locus_tag="pgaptmp_005531"
     CDS             complement(<1..>568)
                     /locus_tag="ACHK0Z_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4505309.1"
                     /translation="AEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARD
                     VRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAR
                     EAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGID
                     GGGLDDLLGMYVTGRDRETREWLGWGHAW"
BASE COUNT          120 a          196 c          142 g          110 t
ORIGIN      
        1 cccaggcatg gccccagccc agccattcgc gggtttccct gtcacggcca gtcacgtaca
       61 ttcccagcag atcatccagg cccccgccgt caatccccac cgtcaccaca tcagcgcgct
      121 gcaggatatc gtccaggctg acgcgcctgc cctgctgctc ccagaaatcc gcgcccgccc
      181 agcggtcaga acgcagggca agaccgattt ccacattggc gtgttttgac atgaagccac
      241 gaaatgcttc ctcaccagcc tcccgggctt tacggtactc ccggtacaga aaggcctcat
      301 ccaccgaata accgagattc gggttaacca tggcgaggtt ttccatcagc aggtgagccc
      361 cgctttccac catttcagga ggatgctcaa aaatcaccgg cagaaagtgc ggatcatgaa
      421 ttttgccgtc acggacatcc cgggcatact gcagtttctg tctgaacacc ccggcgggcg
      481 gttcattcga ctgggtggtc gtatacacca caaacccttc cggacgggag gcaagcccgc
      541 cgatggcttc acgtagcatg tcttccgc
//
LOCUS       DAZFWP020000531          567 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFWP020000531 DAZFWP020000000
VERSION     DAZFWP020000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.540"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            20..241
                     /gene="escF"
                     /locus_tag="ACHK0Z_RS27660"
                     /old_locus_tag="pgaptmp_005532"
     CDS             20..241
                     /gene="escF"
                     /locus_tag="ACHK0Z_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HGU4505310.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            247..525
                     /locus_tag="ACHK0Z_RS27665"
                     /old_locus_tag="pgaptmp_005533"
     CDS             247..525
                     /locus_tag="ACHK0Z_RS27665"
                     /old_locus_tag="pgaptmp_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HGU4505311.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCSLVHENNRSWTLMF"
BASE COUNT          189 a           84 c          117 g          177 t
ORIGIN      
        1 aactattgag gaaaatttaa tgaatttatc tgaaattact caacaaatgg gtgaagtagg
       61 taaaacgctg agtgattctg tgccagagtt acttaatagc accgatttgg ttaatgaccc
      121 tgaaaaaatg ttagagctgc agttcgcggt tcagcaatat tctgcttatg ttaacgtaga
      181 aagtggaatg ctgaaaacga taaaagattt ggtctcaacc atttccaacc gtagctttta
      241 atttttatgg ttaatgatgt ttctgccaac aagatactgg tgtgggcggc tgtagctgcg
      301 gcaaatcata agcttcccaa atatgcagaa tctatcctga atgtattgcc gcaaattata
      361 cccgataaaa aagatatcgc acatttagaa tttattattt tatatggatt aaatagaaaa
      421 aatgatgcgg taaaggctct ggaggacttt atggatgatg agacaagcca gttgttatgc
      481 agtctggtcc acgagaataa tagaagctgg acactaatgt tttaatctca tattgagttt
      541 aaacaattga taaatttcat tttggta
//
LOCUS       DAZFWP020000532          566 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFWP020000532 DAZFWP020000000
VERSION     DAZFWP020000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.541"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>566
                     /locus_tag="ACHK0Z_RS27670"
                     /old_locus_tag="pgaptmp_005534"
     CDS             <1..>566
                     /locus_tag="ACHK0Z_RS27670"
                     /old_locus_tag="pgaptmp_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4505312.1"
                     /translation="KGIETRDSVNQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVA
                     QQEPEKVCTACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPH
                     KENTDGNQHHDSDNETGETADHSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPII
                     SIGAIFFDPQTGDMGPEFSKTIDLETAGG"
BASE COUNT          195 a          135 c          148 g           88 t
ORIGIN      
        1 aaggggattg aaacccgcga ttctgtaaac cagaaccagc aagaatcgga acagaacgac
       61 cagaaagcgg aacaaaacag cccaaatacg caacaaaacg agccagaaac gaaacaacct
      121 gagccagtag cgcaacagga accggaaaaa gtctgcaccg cctgcggtca gaccggcggc
      181 ggcaactgcc ctgattgtgg cgcggtgatg ggcgacgcaa cataccagga aacattcgat
      241 gaagagtatc aggttgaagt tcaggaagat gatccggagg aaatggaagg cgctgaacat
      301 ccacacaagg agaacactga cggcaatcag catcacgata gcgataatga aactggcgag
      361 acggcagatc actcaattaa ggtgaacggt catcaagaaa tcacatccac cagcaggacg
      421 tgtgaccatc taatgatcga ccttgaaacc atgggaaaaa atcctgatgc cccgatcatc
      481 tcaataggtg caatattttt cgatccgcaa accggagata tggggccgga atttagtaag
      541 actatcgatc tggaaactgc tggcgg
//
LOCUS       DAZFWP020000533          565 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFWP020000533 DAZFWP020000000
VERSION     DAZFWP020000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.542"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>565
                     /locus_tag="ACHK0Z_RS27675"
                     /old_locus_tag="pgaptmp_005535"
     CDS             <1..>565
                     /locus_tag="ACHK0Z_RS27675"
                     /old_locus_tag="pgaptmp_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4505313.1"
                     /translation="ARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQ
                     KEQLALAGQNTELAKLKYQTALGELKTLSEIQKQELLRNAALIDQQKIREQLRSREET
                     LKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISED
                     FYRQALAQNAQYLSERLKEQEAFYAESD"
BASE COUNT          167 a          130 c          178 g           90 t
ORIGIN      
        1 gcgagacaac gttatatcag taatgccctg gaagcctggc gcaataacga ggcgaataaa
       61 cccaaatccc ggggtggaaa atcagagacg gaaaaagcgg aagacagttt ttcccggctg
      121 ctgaagcagc agaaagagca actggcactg gccggtcaga acacggagct ggcgaagctg
      181 aagtaccaga cagcgctggg tgaactgaaa accctgtcgg agatacagaa gcaggagctg
      241 ctgcgtaacg cggccctgat tgaccagcaa aaaatccggg aacagttgcg atcccgggaa
      301 gagaccctga agaatgataa tgtggctgcg cgtgcatcaa atgaagccga actgctgggg
      361 tacgggcagg gagaacgagc cagggaacgc atgcgggagt tgcagcagat ccgcgacagc
      421 ttccgccaga aggatgcgga ccttcagtct cagtatcaga ccggggatat cagtgaggat
      481 ttttacagac aggcgctggc gcagaatgca cagtatctga gtgaacgtct gaaagagcag
      541 gaagcctttt atgccgaatc ggatg
//
LOCUS       DAZFWP020000534          557 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFWP020000534 DAZFWP020000000
VERSION     DAZFWP020000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.543"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>557
                     /locus_tag="ACHK0Z_RS27680"
                     /old_locus_tag="pgaptmp_005536"
     CDS             <1..>557
                     /locus_tag="ACHK0Z_RS27680"
                     /old_locus_tag="pgaptmp_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4505314.1"
                     /translation="RARAKFHWQFLEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAE
                     YMQENRWDEESASFVPVETESDPINVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQ
                     ELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRI
                     WKKRREGERKETGKYTSVVDLARAR"
BASE COUNT          151 a          132 c          146 g          128 t
ORIGIN      
        1 gtcgcgcccg cgcaaaattt cactggcaat tcctggaaca ttatccggct gctcaggact
       61 gcgcttataa atttcttgtt tgcgaggata aacccggtat tccccgccct gccctcgatt
      121 cctgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct tcctttgtcc
      181 cggttgagac tgaatcagat ccgataaacg tcacttttga caagctggcc cctgaagtac
      241 agaacgctgt catggttaag ttcgacacat gtgaaaacat caccgttgat atggtgatta
      301 gcgcgcagga actgttgcag gaagacatgg caacattcga cggacatatc gttgaagcgt
      361 tgatgaaaat gccagaagtt aacgccatgt atccggagct taagctgcat gccatcgggt
      421 gggttaagca taaatgtaag ccaggtgcca aatggcccga aattcaggca gagatgcgca
      481 tctggaaaaa acgtcgcgaa ggtgaacgca aggaaaccgg aaaatacacg tctgttgttg
      541 atctcgcccg cgccaga
//
LOCUS       DAZFWP020000535          555 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFWP020000535 DAZFWP020000000
VERSION     DAZFWP020000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.544"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..366)
                     /locus_tag="ACHK0Z_RS27685"
                     /old_locus_tag="pgaptmp_005537"
     CDS             complement(<1..366)
                     /locus_tag="ACHK0Z_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4505315.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHDFTDGTTN
                     QLNISNSLIHGSITSMLPMG"
BASE COUNT          168 a          104 c          119 g          164 t
ORIGIN      
        1 acccataggc agcattgaag tgattgaacc atgaatcagg gagttgctga tgttcaactg
       61 gtttgttgtt ccatcagtaa agtcatgatt caggtaatat gtagagatag tcccgttaac
      121 agtgtcacca ttaaaggtgg tatcgaaatc accgtttgtg ttgttatagt agacaaagcc
      181 actataattc acatagttac catctacagt acccggtgca tccatattgc tccagtcaag
      241 agcggtagtt ggattgtcgt aataagtcca ggtgtcataa gtagttgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaatgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataacctat ggagaaaaac acgcatcaaa
      541 ttgataaaag ggcga
//
LOCUS       DAZFWP020000536          546 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFWP020000536 DAZFWP020000000
VERSION     DAZFWP020000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.545"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          172 a          121 c          104 g          149 t
ORIGIN      
        1 aaggctgggt catatccctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa
       61 taatgatctg gcagcctgag ttcacagata aaatactctc caggaaaccc ggggcggttc
      121 acccctaatt caactaattg gaggcattac catgcattag tgcaagcgcc ttgtggattc
      181 ttttgctaat gacggaaaca tattgtacaa tattgcattt atccctcaat aaataaatga
      241 gttatcagat tgcaaaaatc tgttatcaaa ctaattcaaa taaaaagaga ttgatacgac
      301 caacgtacac aaaaattttc gatattcgaa ctaaataaca gctcctaata ccgctacact
      361 ttagctatcc agtctgcgcc tcagaaatgg acgatgccgt tgaaggactg gtgtatggca
      421 atgagccgct ttattatcga attcggtgac cgcctggacg gtcacttcgg agaaaaggca
      481 tttacacagc atcctaaaca ggttcagcat aggtgatata ccgccttttc attttcttct
      541 ccatta
//
LOCUS       DAZFWP020000537          540 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFWP020000537 DAZFWP020000000
VERSION     DAZFWP020000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.546"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            100..194
                     /locus_tag="ACHK0Z_RS27690"
                     /old_locus_tag="pgaptmp_005538"
     tRNA            100..194
                     /locus_tag="ACHK0Z_RS27690"
                     /old_locus_tag="pgaptmp_005538"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Sec,seq:tca)
     gene            273..434
                     /gene="rhuM"
                     /locus_tag="ACHK0Z_RS27695"
                     /old_locus_tag="pgaptmp_005539"
                     /pseudo
     CDS             273..434
                     /gene="rhuM"
                     /locus_tag="ACHK0Z_RS27695"
                     /old_locus_tag="pgaptmp_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          123 a          143 c          126 g          148 t
ORIGIN      
        1 tacgctgcgt gtattaggcg gaaaaaactg atctggggga tgtagaaact caaggaagta
       61 gctataatgc gccccgcctc catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg
      121 aggcggctgg acttcaaatc cagttggggc cgccagcggt cccgggcagg ttcgactcct
      181 gtgatcttcc gccaaaatgc ctctcctgac gttctccaac cccttgtaat actgtcaatc
      241 cagatatttt ctactgctca atattcaatc ggcttcagga atacctggtc taaggttttg
      301 tgatggacga tgagcggctg aagaatccgc ctgtcggttc ttctgtcctc gaacgcatcc
      361 gcgatattca cgccagtatg cgccgtgttt atttgcaggt gatttgcccc cctgctgact
      421 ctcagccaac tgtatcgcca ttaaggtgaa gatgttgttt tactcgcccc agaacaccct
      481 tttttatacg actattctat ttctttaacc gctggcgaaa aaaaactgac tataaacttc
//
LOCUS       DAZFWP020000538          539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFWP020000538 DAZFWP020000000
VERSION     DAZFWP020000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.547"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>539
                     /locus_tag="ACHK0Z_RS27700"
                     /old_locus_tag="pgaptmp_005540"
     CDS             <1..>539
                     /locus_tag="ACHK0Z_RS27700"
                     /old_locus_tag="pgaptmp_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4505316.1"
                     /translation="DGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLF
                     AVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTDMAGQCVGGSAGGVPWICGDTTYF
                     WKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSK
                     WRDSSATWTSQDRASHFSS"
BASE COUNT          143 a          120 c          165 g          111 t
ORIGIN      
        1 gatggcacat acagtgacgc gagtggtgcc tcggagaatt caacccgctg gggtgtggac
       61 aagccgctgt ataaggacct tatcggtcga acaaaagcag cactgaagaa gaacccgaaa
      121 aatgtgctgt ttgccgtggt gtggatgcag ggggaatttg attttgacgg cacgcccgga
      181 aatcacgcag cacagtttgg tgcgctggtt gataaattcc gtgcagacct gacggatatg
      241 gcaggtcagt gcgttggtgg ctctgctggc ggtgttccct ggatctgtgg agatacgacg
      301 tatttctgga agcagaagaa cgaatcctcg taccagacgg tgtacggcag ctacaaaaac
      361 aaaacggaaa agaatatcca tttcgtaccg ttcatgaccg atgagaacgg ggtgaatgtg
      421 ccgacgaaca aaccggaaga agacccggac attccgggta tcgggtatta cggttcgaaa
      481 tggcgtgaca gctcagccac ctggacgtca caggacaggg cgagccattt cagttcatg
//
LOCUS       DAZFWP020000539          538 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFWP020000539 DAZFWP020000000
VERSION     DAZFWP020000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.548"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..397)
                     /locus_tag="ACHK0Z_RS27705"
                     /old_locus_tag="pgaptmp_005541"
     CDS             complement(<1..397)
                     /locus_tag="ACHK0Z_RS27705"
                     /old_locus_tag="pgaptmp_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4505317.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIID"
     gene            complement(387..>538)
                     /locus_tag="ACHK0Z_RS27710"
                     /old_locus_tag="pgaptmp_005542"
     CDS             complement(387..>538)
                     /locus_tag="ACHK0Z_RS27710"
                     /old_locus_tag="pgaptmp_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGU4505318.1"
                     /translation="RIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSG
                     NNNVK"
BASE COUNT          180 a          118 c           70 g          170 t
ORIGIN      
        1 catcgattat ctcttcattg tcttcagaac tattcacctt ctgaatcttt tcaaaaaagc
       61 taaccaactc aggttcagaa tattttatca aatatgattt ataaccactc atgcctgtct
      121 caacaaaatt aacaaccgat gctacattat tgaatgaaag attctcattc tgactgttat
      181 tatatatttc cttcccaacc ggcatcatta cgaacattga caatagcaaa gcaaccccat
      241 taagtgtcat atttgatggc acttgctgaa gtcccagcgc gtttcggaca ataacaaaaa
      301 caatcgagaa tttaataaaa catgtcccgg atgctattat aaacggcaat agtgtaaata
      361 atgataatat cgctatcagc gaaatgctat ttgacattat tattccctga taaccaagaa
      421 tgaatttcta cgcccaattt atcatccact tcaacaagtt cgccatagcc agttagcgca
      481 ccatttacac gtatctctat atttttctca gattcaggaa gcagagatat tattcttt
//
LOCUS       DAZFWP020000540          534 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFWP020000540 DAZFWP020000000
VERSION     DAZFWP020000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.549"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            85..231
                     /locus_tag="ACHK0Z_RS27715"
                     /old_locus_tag="pgaptmp_005543"
                     /pseudo
     CDS             85..231
                     /locus_tag="ACHK0Z_RS27715"
                     /old_locus_tag="pgaptmp_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(256..>534)
                     /locus_tag="ACHK0Z_RS27720"
                     /old_locus_tag="pgaptmp_005544"
     CDS             complement(256..>534)
                     /locus_tag="ACHK0Z_RS27720"
                     /old_locus_tag="pgaptmp_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505319.1"
                     /translation="LNDINLALSFILVINNTYVNINIGAKRIRDTGCKAIYFVLISLG
                     LTLFSIISAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          158 a          107 c          105 g          164 t
ORIGIN      
        1 catctctgaa gagaacctcg ttaatgattg gtcttgctca ttgttaataa caaaatgttg
       61 aatttaccga ttaggaaact ttttcctacg cggattgcct ccagctatgg catgaacttt
      121 gagtttatgc cagaactgaa gtggggcttc ggctaccctg gcgcgattat ctttatgatc
      181 ctcgcgggcc tgacatcgta tctgtacttt aagcgtaaga actggctgta actatacgat
      241 gtcgctgtga gtaagttagt gtgctgacag cgacatttta gccgagggaa tcgcagataa
      301 cataattgtt gtgaataata tccatgccac gttgagttgg acgcttaatg actcacatct
      361 caccccttca attgctgcag agataataga aaaaagtgtc aagcccaatg aaatcaatac
      421 aaagtagatt gccttacatc cagtatctct tattcttttt gctccaatat tgatatttac
      481 ataggtatta ttaataacca gaataaaact caaagccagg ttgatatcat tcaa
//
LOCUS       DAZFWP020000541          533 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFWP020000541 DAZFWP020000000
VERSION     DAZFWP020000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.550"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>533
                     /locus_tag="ACHK0Z_RS27725"
                     /old_locus_tag="pgaptmp_005545"
     CDS             <1..>533
                     /locus_tag="ACHK0Z_RS27725"
                     /old_locus_tag="pgaptmp_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4505320.1"
                     /translation="CGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVN
                     VPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRH
                     AGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGRAE
                     VVSDAEGTGGKAVKLTK"
BASE COUNT          144 a          130 c          168 g           91 t
ORIGIN      
        1 tgtggagata cgacgtattt ctggaagcag aagaacgaat ccacgtacca gacggtgtac
       61 ggcagctata aaaacaaaac ggaaaagaat atccatttcg taccgttcat gaccgatgag
      121 aacggggtga atgtgccgac gaacaaaccg gaagaagacc cggacattcc gggtatcgga
      181 tattacggtt cgaaatggcg tgacagctca gccacctgga cgtcacagga cagggcgagc
      241 catttcagcg cctgggcacg ccgtgggatt atttccgacc gtctggcaac ggcgattttg
      301 cgccatgcgg gaagagtggc gctaaacgcg ggggcatcat cgacagtatc agaggtgcgc
      361 ccttcatcgc cttccggtgc agaagccaca ggcgtcacaa cactgctctc ttaccttgcc
      421 agcgagtcag agggaagcct gaaagtacag ggatggtcag ccagtggcgg cagggcagaa
      481 gtggtcagcg atgcggaggg aaccggaggt aaggcagtga agctgaccaa gga
//
LOCUS       DAZFWP020000542          530 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFWP020000542 DAZFWP020000000
VERSION     DAZFWP020000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.551"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..252
                     /locus_tag="ACHK0Z_RS27730"
                     /old_locus_tag="pgaptmp_005546"
     CDS             <1..252
                     /locus_tag="ACHK0Z_RS27730"
                     /old_locus_tag="pgaptmp_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4505321.1"
                     /translation="HVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTMLIRELSGRGR"
     gene            249..>530
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS27735"
                     /old_locus_tag="pgaptmp_005547"
     CDS             249..>530
                     /gene="tnpB"
                     /locus_tag="ACHK0Z_RS27735"
                     /old_locus_tag="pgaptmp_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4505322.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLA"
BASE COUNT          119 a          140 c          140 g          131 t
ORIGIN      
        1 catgtttcgc ggcgtcttcc ggtaacaacc tcttctgaca ctggcgttga attattacct
       61 gtagaaataa cgccggatga gcagaaagaa cctgtggcgg ccattgcgcc gtctttatcc
      121 acttccactc agaccagagt cagtgccagt tcctgcaagg tggaattccg tcacggtaac
      181 atgacgctgg aaaatccatc gccagagctg ctcacgatgt taatccgcga actgagcggg
      241 aggggaagat gatttcactc ccatcaggta cccgtatctg gctcgttgcc ggcgttaccg
      301 atatgtgtaa atccttcaac ggactgggag aacaggtaca acatgtgctg aatgataatc
      361 ccttctccgg tcacctgttt atcttccgtg gccgacgggg ggacaccgtt aaaattcttt
      421 gggctgatgc tgatggtctg tgcctgttca ccaaacgcct ggaggaaggc cagtttatct
      481 ggcctgcggt gcgtgacggt aaggtatcca ttacccgctc gcaactggca
//
LOCUS       DAZFWP020000543          525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFWP020000543 DAZFWP020000000
VERSION     DAZFWP020000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.552"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            308..>525
                     /locus_tag="ACHK0Z_RS27740"
                     /old_locus_tag="pgaptmp_005548"
     CDS             308..>525
                     /locus_tag="ACHK0Z_RS27740"
                     /old_locus_tag="pgaptmp_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4505323.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTR"
BASE COUNT          144 a          151 c           99 g          131 t
ORIGIN      
        1 atctcccact gacatctccc ccacaataca ctaaaaaata ctcacagcag taaacacaga
       61 cacacctgat aatcatacag ccgataaata ttttcccctt tacggagaaa tcagcctgct
      121 gtttaacaat aacggcgtat tttcatggaa ggagatcacc cagtaacgga taagtcctga
      181 tctccttcat gttaatacgc aacaatccca tcgattcaaa acaatttcag acagatatta
      241 tctccgtgaa tcccccgcca cctttccggt gcgcggggtt ttgtcttttt tcaccgggaa
      301 tacatgtatg aatccgtctg atgccattga ggcaattgaa aaaccgctct cctccctgcc
      361 ttactcactt tcccgtcaca tcctggaaca tctgcgcaaa ctcaccagtc acgaacccgt
      421 gattggcatt atgggtaaaa gcggggccgg taaatcctca ctctgtaatg cactgtttca
      481 gggggaggtc accccggtca gtgatgttca cgccggcacc cggga
//
LOCUS       DAZFWP020000544          520 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFWP020000544 DAZFWP020000000
VERSION     DAZFWP020000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.553"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(59..355)
                     /locus_tag="ACHK0Z_RS27745"
                     /old_locus_tag="pgaptmp_005549"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHK0Z_RS27745"
                     /old_locus_tag="pgaptmp_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          130 a          131 c          111 g          148 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atctacaaca
      421 gcgtattttg cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat
      481 ttcccattga agcgatgtgt caggtatttg gtgtatccag
//
LOCUS       DAZFWP020000545          508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFWP020000545 DAZFWP020000000
VERSION     DAZFWP020000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.554"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            22..240
                     /gene="ygeI"
                     /locus_tag="ACHK0Z_RS27750"
                     /old_locus_tag="pgaptmp_005550"
     CDS             22..240
                     /gene="ygeI"
                     /locus_tag="ACHK0Z_RS27750"
                     /old_locus_tag="pgaptmp_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HGU4505324.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            323..>508
                     /locus_tag="ACHK0Z_RS27755"
                     /old_locus_tag="pgaptmp_005551"
     CDS             323..>508
                     /locus_tag="ACHK0Z_RS27755"
                     /old_locus_tag="pgaptmp_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU4505325.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNR"
BASE COUNT          193 a           95 c           73 g          147 t
ORIGIN      
        1 acaattaaaa aagggagctt tatgactaat ccaatcggta tcaataattt atctcaaagt
       61 tcaaatatag cgaatgcaac gggtgatgaa gtcgtatcat tagataaaca catcaacacc
      121 agtgctactg atacagatca aatacaagca tttatagttt caacatggat ggcgcctttt
      181 caaaatgata tgtattcaga agataatcct atctcacctt actataaaat tgaatggtag
      241 acgattgacc aaataaatca taacgagata cttttataat agtatctcgt ccctttcatt
      301 caatcctcac acatgaaaaa atatgcgctc accaaaagtt aaattcttaa ccatcttcac
      361 gttttgtata tttattacaa aaatgtcttt cgccagcaac tcttgctcta atgaagcagg
      421 aacaatgttc agaatcgaac caaatttaat taaagctatc gctcttgttg aaagcaatct
      481 aaaaaaggat agcataggca agaacaga
//
LOCUS       DAZFWP020000546          507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFWP020000546 DAZFWP020000000
VERSION     DAZFWP020000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.555"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>507
                     /locus_tag="ACHK0Z_RS27760"
                     /old_locus_tag="pgaptmp_005552"
     CDS             <1..>507
                     /locus_tag="ACHK0Z_RS27760"
                     /old_locus_tag="pgaptmp_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4505326.1"
                     /translation="ELKKNGVVDKTGHTWFLAGEGESGVTEEQPAQSEVPDVLTGEVE
                     QKVTADMMIEFICQDGAKTCEELADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRY
                     CIPGADLPAEPEAASVAETDGKAFPQPTGVALPVQETTTQEEMKTEIVEDIVKLQPSV
                     TETKADDLI"
BASE COUNT          148 a          108 c          149 g          102 t
ORIGIN      
        1 gaactcaaaa aaaatggcgt tgttgataaa acgggtcaca cctggtttct ggctggtgaa
       61 ggtgaatccg gggtaaccga agaacagcca gcacagtctg aagtaccgga tgtgctgacc
      121 ggggaggtcg aacaaaaagt taccgcggac atgatgattg agtttatctg tcaggatggg
      181 gctaaaacgt gtgaggaact ggcggataag ttcggtgtta gcattcgcaa ggttgcttcc
      241 acgttggcgg tcgtaacagc aacggggcgc ctggcacgcg taaatcagaa cggtaaattt
      301 cgttactgca taccgggcgc tgatttaccg gcagagccgg aagctgcatc cgtagcggaa
      361 accgatggta aagcctttcc tcagccaaca ggtgttgcgt taccagtcca ggaaacgacg
      421 acacaggaag aaatgaaaac agaaatcgtg gaagacattg tgaagttaca gccatcggtc
      481 accgaaacga aagcagatga cctgatt
//
LOCUS       DAZFWP020000547          504 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFWP020000547 DAZFWP020000000
VERSION     DAZFWP020000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.556"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>504
                     /locus_tag="ACHK0Z_RS27765"
                     /old_locus_tag="pgaptmp_005553"
     CDS             <1..>504
                     /locus_tag="ACHK0Z_RS27765"
                     /old_locus_tag="pgaptmp_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HGU4505327.1"
                     /translation="NGLKLLQKQADLLKIIMEDTSVTENTFKNIEMAITDIKREYYSH
                     TVDIEKNIHAIWVAGSPPESISDYIKTFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQI
                     AFDLACRTIQQNTPQKNIDFINLYNEIRKKYNNNPSGQQEYLNKLRELYATYQKISTP
                     LKHMFNS"
BASE COUNT          197 a           90 c           78 g          139 t
ORIGIN      
        1 gtaatggtct aaaacttcta cagaagcaag ctgatttact aaaaataatc atggaggata
       61 cctctgttac agaaaatacc tttaaaaaca tagagatggc tataacagat ataaaaaggg
      121 agtactattc gcatacagtt gatattgaga aaaatattca tgccatatgg gttgcgggtt
      181 ccccacctga aagcatctcg gactatatta agacttttct caaaacctac aaagagttta
      241 cttactatct ttgggttgat gaaaaagcat ttggagccgc gaaatttacc agtgtcttaa
      301 aacaaattgc atttgattta gcatgcagaa ctatacagca gaatactcca caaaaaaata
      361 ttgattttat taatctatat aatgaaataa ggaagaaata caacaacaac ccatcgggac
      421 aacaagagta cttaaacaaa ctcagagagc tttatgctac ttatcaaaaa atatccaccc
      481 ctctgaaaca catgtttaat tcat
//
LOCUS       DAZFWP020000548          503 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFWP020000548 DAZFWP020000000
VERSION     DAZFWP020000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.557"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..137
                     /locus_tag="ACHK0Z_RS27770"
                     /old_locus_tag="pgaptmp_005554"
                     /pseudo
     CDS             1..137
                     /locus_tag="ACHK0Z_RS27770"
                     /old_locus_tag="pgaptmp_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(332..445)
                     /locus_tag="ACHK0Z_RS27775"
                     /old_locus_tag="pgaptmp_005555"
     CDS             complement(332..445)
                     /locus_tag="ACHK0Z_RS27775"
                     /old_locus_tag="pgaptmp_005555"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4505328.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          157 a          130 c          111 g          105 t
ORIGIN      
        1 taatgtttaa tcgacatcga atttctcccg tgtagaggaa cagagttaaa aagccggaag
       61 cggaatcaaa tcacaggatg accatctgcc agtggctggt cgtaaaaaaa aggccgcgcc
      121 atgcgcagcc gaaaataaag ggataacgat gatagtttga gaaaaacaga aataacactt
      181 ttgtggcaaa gcatggtgcc gggtgcctcc cggtgaattc agtatcagca cctgaatccg
      241 cgattacccc atattccttc ttgctgattg ccccaccgca cagggggatt caccatgcag
      301 aagtgttttt aataaacagc aaacaaaaaa atcaagcatt atgcaggctg tttcttttta
      361 tcaccggcca cagcaatacc ataatgccgc agaccagcac cccatccgcc agcaccgaca
      421 tgattctgct ggtgaaatcc accatcacca ccagaaacag caggagtgca gccacagtca
      481 ggcgcagttt taccgtcaca ggt
//
LOCUS       DAZFWP020000549          502 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFWP020000549 DAZFWP020000000
VERSION     DAZFWP020000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.558"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..351)
                     /locus_tag="ACHK0Z_RS27780"
                     /old_locus_tag="pgaptmp_005556"
     CDS             complement(<1..351)
                     /locus_tag="ACHK0Z_RS27780"
                     /old_locus_tag="pgaptmp_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505329.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSF"
     gene            complement(355..>502)
                     /locus_tag="ACHK0Z_RS27785"
                     /old_locus_tag="pgaptmp_005557"
     CDS             complement(355..>502)
                     /locus_tag="ACHK0Z_RS27785"
                     /old_locus_tag="pgaptmp_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505330.1"
                     /translation="IIAHRLSTVKNANRIIVMDNGFISEDGTHKELISKKDSLYAYLY
                     QLQA"
BASE COUNT          170 a          100 c           72 g          160 t
ORIGIN      
        1 aaaagagcca tcttttacca ttattttttc tacaatcgca ttctctatcg gttttatttc
       61 tttgcttctt ccactatgag taaacttacc atttgctact gaaaccactt caactttccc
      121 aagaacagat aagagaagag atataattac aaataataaa atactccata caacaaaatg
      181 agaacgtctg gatactggtg tttcaatgag ttcaaggtga gcaggaagaa atgcatactc
      241 atctttttct ctcacaggag catcaagtgt gtgacgaatt ttccagacat cagaaaaaac
      301 cgtcttataa cggcaaacca aatcccataa acctttcatg taaaagcgca tacgttatgc
      361 ctgcaactga tataaatatg catataaact gtcttttttg gagataagct ctttatgtgt
      421 gccatcttca gaaataaagc cattatccat aacaattatt ctgtttgcat tttttacagt
      481 tgacaacctg tgtgcaataa ta
//
LOCUS       DAZFWP020000550          501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFWP020000550 DAZFWP020000000
VERSION     DAZFWP020000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.559"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>501
                     /locus_tag="ACHK0Z_RS27790"
                     /old_locus_tag="pgaptmp_005558"
     CDS             <1..>501
                     /locus_tag="ACHK0Z_RS27790"
                     /old_locus_tag="pgaptmp_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4505331.1"
                     /translation="PEIKPSPGGNTPSGPSADTSVRTISLLPAAGEAAAQGWSIKDGG
                     IQLSDGVFKITKQSNKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYL
                     YTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTL
                     ELVFTAG"
BASE COUNT          113 a          137 c          150 g          101 t
ORIGIN      
        1 ccggaaatca aaccctcgcc cggcggcaac acgccatcgg gtccgtctgc agatacgtcc
       61 gttcgcacaa tctccctgct gccggcagcc ggagaggctg ctgcgcaggg ctggagcatt
      121 aaggatggcg gaattcagtt gtcagatggt gtatttaaga tcaccaagca gagcaataaa
      181 acctggtccc tgacgcatcc ggtggatgac gcaattaccc tgctgacaca gggcggcaga
      241 ctgacctgta agttccgcct gtcaggcgca ctgaccaaca atcagttcgg gctggggatt
      301 tatctgtata cggatgctcc cgttcctgat ggtgtggcga tgacgggtac cggtaatccg
      361 ttcctgatgt cgtacttcac tcagaccact gacggcagag tgaatctgat gcatcacagg
      421 aaagccggaa acacgaagct gggggagttc ggcgattacg gtaacgactg gcagacgctg
      481 gagctggtgt tcaccgccgg c
//
LOCUS       DAZFWP020000551          500 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFWP020000551 DAZFWP020000000
VERSION     DAZFWP020000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.560"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..456)
                     /locus_tag="ACHK0Z_RS27795"
                     /old_locus_tag="pgaptmp_005559"
     CDS             complement(<1..456)
                     /locus_tag="ACHK0Z_RS27795"
                     /old_locus_tag="pgaptmp_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4505332.1"
                     /translation="MATLRELIIKTSANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLK"
BASE COUNT          117 a          151 c          122 g          110 t
ORIGIN      
        1 cttcagcaca tcatcagcac tgtaaccata ttcacgcatc gaggcagccg aacgggcaaa
       61 cagggccgca ttatccgaaa atgccgtgcc tgtccgctga ctgatatcca tcagcacttt
      121 ctgtgatgac gaaaattcat cggatgactg tgatgcctgt ttcagacggg cattcacgga
      181 actccactca tccgccagag aaatcaggtg tccggtggca aaggcaccgg caaacgcacc
      241 ggtcattccg acagccgaag cgcggatttc cgtcaactgg ctgttcagtt ctgccagggc
      301 acgtcgctgc tccctggctg ccgcagcagc ctgacgtccg ccattctgca gggtccggta
      361 atattcactg cccatacggg acgcccgctg gatctccgac tggaatgact gtgaatttgc
      421 cgaagttttg ataatcagtt cacgtaacgt cgccattcac ctttctccgg gcaaaaaaaa
      481 cctgccacag caggttttca
//
LOCUS       DAZFWP020000552          499 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFWP020000552 DAZFWP020000000
VERSION     DAZFWP020000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.561"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            294..458
                     /locus_tag="ACHK0Z_RS27800"
                     /old_locus_tag="pgaptmp_005560"
     CDS             294..458
                     /locus_tag="ACHK0Z_RS27800"
                     /old_locus_tag="pgaptmp_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505333.1"
                     /translation="MKKREEVSKLPRRRDAALAVPYKKDEFISPSDDKKFSKAKSFTS
                     TSLKDKYFKI"
BASE COUNT          163 a          106 c          103 g          127 t
ORIGIN      
        1 gtagatccaa atgaaagata tcaatccgta ctagatgtac taaacgatct ctcagctata
       61 agtgatggcg ttcttgactg gcgtctacag atgacgaaac caactaacgg cacatgcgaa
      121 tggcaaaaaa agtctgggga cgctatactg tctatagttt ttgacgcaga aaattcgtct
      181 actactggtt ttcgtttata cgatgatggg cggaaaaggc gtgctacgaa cttaacaata
      241 tcctcaggat gtacccctac aaaactgtat aggttattaa aggataactg atcatgaaaa
      301 agcgcgagga agtaagcaag ctgcctcgca gacgtgatgc agcattagcg gttccctaca
      361 aaaaagatga gttcataagc ccttctgatg acaaaaaatt ttcaaaggcg aaaagtttta
      421 catctacatc tctaaaagat aaatacttta aaatctagcc cggcctcagc gccgggtttt
      481 ctttgcctca cgatcccca
//
LOCUS       DAZFWP020000553          499 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFWP020000553 DAZFWP020000000
VERSION     DAZFWP020000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.562"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            311..>499
                     /locus_tag="ACHK0Z_RS27805"
                     /old_locus_tag="pgaptmp_005561"
     CDS             311..>499
                     /locus_tag="ACHK0Z_RS27805"
                     /old_locus_tag="pgaptmp_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4505334.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKED"
BASE COUNT          129 a          104 c          132 g          134 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgcccact ctgaaggacg
       61 ctggctaaaa cgggtagatg tacttcacat gatacttata tttggcagta cattttctga
      121 cagacagtga cggatgttgt caagatattg tgtcatttat aacctgaacc aggggggccg
      181 gaatgttatc tggcattttt agcagagcct gaatgccata atcacggctc ccggagttgg
      241 ccgtcagtgg gtgacactgg cggctttttg ttttccttta ctttcatttt ctgtcggcgg
      301 tgacggagac atacatcaga tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc
      361 ggtagggacg ggatactggt tactgcaact gctggacaaa gtctctccgt cccagtgggt
      421 ggcaataggt gtgctgggga gtctgctgtt tggcctgctg acgtatctga caaaccttta
      481 tttcaagatt aaagaagat
//
LOCUS       DAZFWP020000554          486 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFWP020000554 DAZFWP020000000
VERSION     DAZFWP020000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.563"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>486
                     /locus_tag="ACHK0Z_RS27810"
                     /old_locus_tag="pgaptmp_005562"
     CDS             <1..>486
                     /locus_tag="ACHK0Z_RS27810"
                     /old_locus_tag="pgaptmp_005562"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4505335.1"
                     /translation="LSVGRLTDAKDYPNLLKALLELPERYKLVIIGEGEARNEVERTI
                     LNYGLEKRVKLLGSIANVATYYSACDIYVSSSKWEGFGLVVAEAMSCQRLVVATNAGG
                     VAEVVGDMNYIVPVSDPQLLANKINEVMEFDHETKMEVMYRNREFVLKMFSIDMIVNQ
                     W"
BASE COUNT          151 a           64 c          113 g          158 t
ORIGIN      
        1 actttctgta ggccgactta ctgatgcaaa agattatcca aacttattaa aagcattgtt
       61 agaattacct gaacgatata aattagtcat aataggtgaa ggtgaagcca gaaatgaagt
      121 cgagcgtacg attttaaatt acggccttga aaaaagagtg aaattgctag gtagtattgc
      181 taatgtcgcc acttattatt ctgcatgtga tatttatgta tcgtcctcta agtgggaagg
      241 ctttggtttg gttgtagctg aggctatgtc ttgccagcga ttggtcgttg caactaatgc
      301 cggtggggtg gctgaagttg ttggtgatat gaactatatt gtacctgttt cagaccctca
      361 gttgttggca aataaaatta atgaagttat ggagtttgat cacgaaacta aaatggaagt
      421 aatgtataga aatagagagt ttgttttaaa aatgttctca atagatatga tcgttaatca
      481 atggat
//
LOCUS       DAZFWP020000555          485 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFWP020000555 DAZFWP020000000
VERSION     DAZFWP020000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.564"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            74..370
                     /locus_tag="ACHK0Z_RS27815"
                     /old_locus_tag="pgaptmp_005563"
     CDS             74..370
                     /locus_tag="ACHK0Z_RS27815"
                     /old_locus_tag="pgaptmp_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4505336.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIGHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            376..>485
                     /locus_tag="ACHK0Z_RS27820"
                     /old_locus_tag="pgaptmp_005564"
     CDS             376..>485
                     /locus_tag="ACHK0Z_RS27820"
                     /old_locus_tag="pgaptmp_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4505337.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKG"
BASE COUNT          125 a          124 c          127 g          109 t
ORIGIN      
        1 cccctttcct gttttcctaa tcagcccggc atttcgcggg cgatattttc acagctattt
       61 caggagttcg gccatgaacg cttattacat tcaggatcgt cttgaggctc agagctgggc
      121 gcgtcactac cagcagatag cccgtgaaga gaaagaggca gaactggcag acgacatgga
      181 aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc ggtcatttgc aacgccacgg
      241 ggccagcaaa aaagccatta cccgtgcgtt tgatgacgat gttgagtttc aggagcgcat
      301 ggcagaacac atccggtaca tggttgaaac cattgctcac catcaggttg atattgattc
      361 agaggtataa aacggatgag tacagcactc gcaacgctgg cagggaagct ggctgaacgt
      421 gtcggcatgg attctgtcga cccacaggaa ctgatcacca ctcttcgcca gacggcattt
      481 aaagg
//
LOCUS       DAZFWP020000556          483 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFWP020000556 DAZFWP020000000
VERSION     DAZFWP020000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.565"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            175..>483
                     /gene="gadE"
                     /locus_tag="ACHK0Z_RS27825"
                     /old_locus_tag="pgaptmp_005565"
     CDS             175..>483
                     /gene="gadE"
                     /locus_tag="ACHK0Z_RS27825"
                     /old_locus_tag="pgaptmp_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU4505338.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     Y"
BASE COUNT          172 a           70 c           94 g          147 t
ORIGIN      
        1 tatatggagt gcgtgatgga taaatctgaa gtattgatta gtgttaatag acgtattagt
       61 tcacgaaggg caaagttctt ataggcgttt actatattga acaacgattc ggacaaggat
      121 gtaaataatg aaaaggatga catgttcgaa acgataacgg ctaaggagca agttatgatt
      181 tttctcatga cgaaagattc ttttctttta cagggctttt ggcagttgaa agataatcac
      241 gaaatgataa aaatcaattc cctgtcagag atcaaaaaag taggcaataa acccttcaag
      301 gttatcattg atacctatca caatcatatc cttgatgaag aagcgattaa atttctggag
      361 aaattagatg ccgagagaat tattgttttg gcaccttatc acatcagtaa actaaaagct
      421 aaagcgccta tttattttgt tagccgcaaa gaaagtatca aaaatcttct tgagattact
      481 tat
//
LOCUS       DAZFWP020000557          482 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFWP020000557 DAZFWP020000000
VERSION     DAZFWP020000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.566"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          143 a          103 c          103 g          133 t
ORIGIN      
        1 accttgtgct gccgcagcca tagggaacgc aagttgagtt atcaggcaga tacccgcagt
       61 taagcggcgt agtttaattc ccatcccgca taagatttgt cggtcattta tttcttctcc
      121 acttctcttc ttcgtagcca tgcttacctt ccctgaacga gctaacataa atgcgccctt
      181 tctagcacag cgaaaaaatg taaatcctga tagcaatatg attgaaaatt aataactttt
      241 cgttaggcag ttttgggtgt gagttgcaag aggggagact actgaataac tcaagtttta
      301 taatcgaggg gaaaatggtg atggcgttca tagcaaaacg ccctcaacca taaaggtcga
      361 gggcgcttaa gatgttaaaa acccgctatc cgttaaaaaa caatgttcaa ctaaggtcag
      421 tgacattgcg ctaaaaaagc gaatttttag aatttggctc ctctgactgg actcgaacca
      481 gt
//
LOCUS       DAZFWP020000558          480 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFWP020000558 DAZFWP020000000
VERSION     DAZFWP020000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.567"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..366
                     /locus_tag="ACHK0Z_RS27830"
                     /old_locus_tag="pgaptmp_005566"
     CDS             <1..366
                     /locus_tag="ACHK0Z_RS27830"
                     /old_locus_tag="pgaptmp_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGU4505339.1"
                     /translation="GPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAY
                     TAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAE
                     AVKALGFLKQKATEQKVAA"
     gene            363..>480
                     /locus_tag="ACHK0Z_RS27835"
                     /old_locus_tag="pgaptmp_005567"
     CDS             363..>480
                     /locus_tag="ACHK0Z_RS27835"
                     /old_locus_tag="pgaptmp_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4505340.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHN"
BASE COUNT          130 a          122 c          129 g           99 t
ORIGIN      
        1 gggccgtggc agtcgcatcc taaacggatg ttacggcata aagctatgat tcagtgtgcc
       61 cgtctggcct tcggatttgc tggtatctat gacaaggatg aagccgagcg cattgtcgaa
      121 aatactgcat acactgcaga acgccagccg gaacgcgaca tcactccggt taacgatgaa
      181 accatgcagg agattaacac tctgctaatc gccctggata aaacatggga tgacgactta
      241 ttgccgctct gttcccagat atttcgccgc gacattcgcg catcgtcaga actgacacag
      301 gccgaagcag tgaaagctct tggattcctg aaacagaaag ccactgagca gaaggtggca
      361 gcatgacacc ggacattatc ctgcagcgta ccgggatcga cgtgagagct gtcgaacagg
      421 gggatgatgc gtggcacaaa ttacggctcg gcgtcatcac agcttcagaa gttcataacg
//
LOCUS       DAZFWP020000559          471 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFWP020000559 DAZFWP020000000
VERSION     DAZFWP020000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.568"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>471)
                     /locus_tag="ACHK0Z_RS27840"
                     /old_locus_tag="pgaptmp_005568"
     rRNA            complement(<1..>471)
                     /locus_tag="ACHK0Z_RS27840"
                     /old_locus_tag="pgaptmp_005568"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          152 c          100 g          132 t
ORIGIN      
        1 ccttctcccg aagttacggc accattttgc ctagttcctt cacccgagtt ctctcaagcg
       61 ccttggtatt ctctacctga ccacctgtgt cggtttgggg tacgatttga tgttacctga
      121 tgcttagagg cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc
      181 atcacgcctc agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc
      241 gggacaaccg tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag
      301 tacaggaata ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga
      361 ctcaccctgc cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt
      421 cacccgcttt atcgttactt atgtcagcat tcgcacttct gatacctcca g
//
LOCUS       DAZFWP020000560          467 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFWP020000560 DAZFWP020000000
VERSION     DAZFWP020000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.569"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>467
                     /locus_tag="ACHK0Z_RS27845"
                     /old_locus_tag="pgaptmp_005569"
     CDS             <1..>467
                     /locus_tag="ACHK0Z_RS27845"
                     /old_locus_tag="pgaptmp_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505341.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRN"
BASE COUNT          103 a          117 c          130 g          117 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatat
//
LOCUS       DAZFWP020000561          467 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFWP020000561 DAZFWP020000000
VERSION     DAZFWP020000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.570"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>467
                     /locus_tag="ACHK0Z_RS27850"
                     /old_locus_tag="pgaptmp_005570"
     CDS             <1..>467
                     /locus_tag="ACHK0Z_RS27850"
                     /old_locus_tag="pgaptmp_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HGU4505342.1"
                     /translation="NNNEQLFMGFDFYRINRFGRWEKYYYIQLRGAFLSAIHHQIIEN
                     QLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAI
                     GRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLT"
BASE COUNT          173 a           79 c           82 g          133 t
ORIGIN      
        1 ttaacaataa tgaacaatta tttatgggat ttgacttcta tcgaataaat agatttggta
       61 gatgggaaaa gtattattat atacaactaa gaggcgcttt tttatcggct attcatcacc
      121 agatcattga aaaccaactg gatacagaaa caataactat tagttatgaa tttatcctct
      181 gtcaacatct tatcgcaaat accgagttca gctatttggc actccctgaa aattataacc
      241 gtttgttttt accaaattca aaaaaccaaa caaataatcg tttcaaaacg ttaaacagca
      301 aagctattgg caggctactt gctgctggtg gcgtatacaa tgggaacatt gaaggattca
      361 gagatactgc ggaaaaactg ggtggagatg caataaaagg ctatgatcaa atactaaatg
      421 aaaaaacagc gggcatagcg atagcaacag catctattct tttaaca
//
LOCUS       DAZFWP020000562          466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFWP020000562 DAZFWP020000000
VERSION     DAZFWP020000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.571"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>466)
                     /gene="espL2"
                     /locus_tag="ACHK0Z_RS27855"
                     /old_locus_tag="pgaptmp_005571"
     CDS             complement(<1..>466)
                     /gene="espL2"
                     /locus_tag="ACHK0Z_RS27855"
                     /old_locus_tag="pgaptmp_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HGU4505343.1"
                     /translation="NGHAQAIKSYGNLVLDTIDKNIDLEYLLSAFKYEAHSSNKYTPG
                     LFSAFQNGHADAIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGLLLAYQNGDINTIQ
                     SFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISHRHDHVVKLYGRLFKKLD"
BASE COUNT          156 a           92 c           59 g          159 t
ORIGIN      
        1 tatcaagttt tttaaataac cttccgtata atttgacaac atgatcatgt ctatgactga
       61 tcgctaatga taaccccgta aaatcataat gttttgcagt caataactct tcgataaaat
      121 cctttgatat atctaacatt attagtgagt caaaaaaaga ctgaatcgta ttaatatctc
      181 cattttgata cgcaagaagt aatcctggtg caccatcata atttctcgcc tcaagcatcc
      241 tgattatctc acctcttttc aaattactat tgccaagtac gccacaatat gcctttattg
      301 cgtctgcatg accattttga aatgcagaaa ataatccagg agtatattta ttagagctat
      361 gtgcctcata tttaaatgct gaaagcaaat actctaaatc tatattttta tcaatagtat
      421 ccagaactaa gttaccataa ctcttaatag cttgagcatg tccatt
//
LOCUS       DAZFWP020000563          465 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFWP020000563 DAZFWP020000000
VERSION     DAZFWP020000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.572"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>465)
                     /locus_tag="ACHK0Z_RS27860"
                     /old_locus_tag="pgaptmp_005572"
     CDS             complement(<1..>465)
                     /locus_tag="ACHK0Z_RS27860"
                     /old_locus_tag="pgaptmp_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HGU4505344.1"
                     /translation="LQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEV
                     YLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVD
                     MLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNF"
BASE COUNT          167 a          102 c           67 g          129 t
ORIGIN      
        1 cgaaattctg ggaaaaagct ttatgataag gaatactgaa aattggaaat gattcagaaa
       61 aaagattgct caatgaatac ccaaatgtat tttgaacttc tccatcctta tataaaaaaa
      121 aggacatcca gttagttgat gtattaggag ataacatatc aaccatatga ctcaaagaac
      181 acatcattgc cataccatca tcctgtgatg ataacaaaac ccagttataa gccaaatcgg
      241 aatcatcatc ccagtcaggc ttcccagagc catcgcacaa tccaaaatca ctctgctgtg
      301 tgacaggata aactttttca ttttttaaat atacttcata gagttcccta aatcgttcct
      361 gcattccatc acgccgtgaa ccattcatta caatttgtat aaaggaacta ttataagata
      421 ccatcgcacc tggatgtcgc tcaaaataca tccctgcctg caata
//
LOCUS       DAZFWP020000564          460 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFWP020000564 DAZFWP020000000
VERSION     DAZFWP020000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.573"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>460)
                     /locus_tag="ACHK0Z_RS27865"
                     /old_locus_tag="pgaptmp_005573"
     CDS             complement(<1..>460)
                     /locus_tag="ACHK0Z_RS27865"
                     /old_locus_tag="pgaptmp_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4505345.1"
                     /translation="FFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKHC
                     GVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYI
                     HKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFG"
BASE COUNT          154 a           95 c           70 g          141 t
ORIGIN      
        1 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
       61 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg tctctcgtat
      121 ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa gccatgtttt
      181 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
      241 aaacgagcaa tgcataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
      301 tgttggcatt ttgtgggtta atcacaccac agtgtttata aaagtctatt ttagaaaaca
      361 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
      421 gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa
//
LOCUS       DAZFWP020000565          457 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFWP020000565 DAZFWP020000000
VERSION     DAZFWP020000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.574"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..453
                     /locus_tag="ACHK0Z_RS27870"
                     /old_locus_tag="pgaptmp_005574"
     CDS             <1..453
                     /locus_tag="ACHK0Z_RS27870"
                     /old_locus_tag="pgaptmp_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HGU4505346.1"
                     /translation="QALKDHLAAYEKSKSLEASGLKPARPAPPPPTSGQVSGSSRSLP
                     PIAQALKDHLAAYEKSKSLDTSSLKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLA
                     AYEKSKSLDTSGLKPSRPAPPPPTSGQASEASRPLPPIAQALKDKNRY"
BASE COUNT          119 a          134 c          107 g           97 t
ORIGIN      
        1 caggcattaa aagatcattt ggctgcctat gaaaaatcga aaagccttga ggcttcaggc
       61 cttaagccgg cccgcccggc tccaccgccg ccgacaagtg ggcaggtatc tggatcatcc
      121 cggtctttac cgcccattgc tcaggcatta aaagatcatt tagctgccta tgaaaagtcg
      181 aaaagtcttg atacttcaag ccttaagcca agccgcccgg caccgccgcc gccgacaagc
      241 gggttggtat ctgggtcatc ccggtcttta ccgcccattg ctcaggcgct aaaagatcat
      301 ttggctgcct atgaaaaatc gaaaagtctt gatacttcag gtcttaagcc aagccgcccg
      361 gcaccgccgc caccgacaag tggacaggca tccgaggcat cccggccatt accgcctatt
      421 gctcaggcat taaaagataa aaaccgatat taagttg
//
LOCUS       DAZFWP020000566          434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFWP020000566 DAZFWP020000000
VERSION     DAZFWP020000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.576"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            183..>434
                     /locus_tag="ACHK0Z_RS27875"
                     /old_locus_tag="pgaptmp_005575"
     CDS             183..>434
                     /locus_tag="ACHK0Z_RS27875"
                     /old_locus_tag="pgaptmp_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4505347.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVIN"
BASE COUNT          107 a          106 c          107 g          114 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taat
//
LOCUS       DAZFWP020000567          434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFWP020000567 DAZFWP020000000
VERSION     DAZFWP020000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.577"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>434)
                     /locus_tag="ACHK0Z_RS27880"
                     /old_locus_tag="pgaptmp_005576"
     CDS             complement(<1..>434)
                     /locus_tag="ACHK0Z_RS27880"
                     /old_locus_tag="pgaptmp_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGU4505348.1"
                     /translation="LNNTPINNPVLRSITNEMIVLQYNLSVEHFNLNSSLIYYINNWN
                     LFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLSAIPVKINEKARYKPGIASYHNI
                     ITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLAS"
BASE COUNT          151 a           68 c           82 g          133 t
ORIGIN      
        1 ttgatgcaag tggtaacggc aattgtatgt gatcaacaaa atcaggaaat aataaattaa
       61 atcgttgctg cattgccaat ggttgttgcc ttatataggg tgacaacgtt gaaaaaccac
      121 atgtgatgat attgtgatag ctagcgatgc cgggtttgta tcgtgccttt tcatttattt
      181 tcaccggtat agctgataaa taatccctta gcacagcagg aaccttataa aaaaatccgc
      241 gttctgcaaa cctttctcga taaaagtgat aaccactgaa taaacagaac aatggaaata
      301 aattccaatt gtttatgtag taaataagtg aactattcag attaaaatgt tcaactgaaa
      361 gattgtactg cagaacaatc atttcattgg taatagatct aagcaccgga ttgttaatag
      421 gtgtattatt caag
//
LOCUS       DAZFWP020000568          426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFWP020000568 DAZFWP020000000
VERSION     DAZFWP020000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.578"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>426
                     /locus_tag="ACHK0Z_RS27885"
                     /old_locus_tag="pgaptmp_005577"
     CDS             <1..>426
                     /locus_tag="ACHK0Z_RS27885"
                     /old_locus_tag="pgaptmp_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4505349.1"
                     /translation="RQAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHL
                     ISLADEWSSVNARLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMRE
                     YGYSADDVLKVTEAISTGLKISGASTAEAGSVITQFSQAL"
BASE COUNT           86 a          110 c          136 g           94 t
ORIGIN      
        1 cgtcaggccg ctgcggcagc ccgggagcag cgacgtgccc tggcagaact gaacagccag
       61 ttgacggaaa ttcgcggttc tgctgtcgga atggctggcg catttgccgg tgcctttgcc
      121 accggacacc tgatttcact ggctgatgag tggagttccg tgaatgcccg tctgaaacag
      181 gcgtcgcagt catctgatga attttcgtca tcacagaaag tgctgatgga tatcagtcag
      241 cggacgggca cggcattttc agataatgcg gccctgtttg cccgctcggc agcctcaatg
      301 cgtgaatatg gttacagtgc tgatgatgtg ctgaaggtga cggaggccat ttcgacaggg
      361 ctgaaaatct ccggtgccag tacggcagag gcgggctcgg tgatcaccca gttcagtcag
      421 gcgctg
//
LOCUS       DAZFWP020000569          422 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFWP020000569 DAZFWP020000000
VERSION     DAZFWP020000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.579"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..295
                     /locus_tag="ACHK0Z_RS27890"
                     /old_locus_tag="pgaptmp_005578"
     CDS             <1..295
                     /locus_tag="ACHK0Z_RS27890"
                     /old_locus_tag="pgaptmp_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4505350.1"
                     /translation="NVSVVLREPSAEAWYLWQEVLNGDGEDDDTLSVVAKTRRNLEAD
                     VTLFCDVLCDTDLQRVFTPDDREQVLAVYGPVHARLLRQALELIADAESARKK"
     gene            319..>422
                     /locus_tag="ACHK0Z_RS27895"
                     /old_locus_tag="pgaptmp_005579"
     CDS             319..>422
                     /locus_tag="ACHK0Z_RS27895"
                     /old_locus_tag="pgaptmp_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505351.1"
                     /translation="MRLALRLGRTLSELRHSLSASEAMMWMEFDRVSPL"
BASE COUNT           81 a          107 c          144 g           90 t
ORIGIN      
        1 aaatgtcagc gtggtgctgc gggagccttc ggcagaggcc tggtatctgt ggcaggaagt
       61 gctcaatggt gatggagagg atgacgatac cctgtcggtg gtggcgaaaa cccgccgtaa
      121 cctggaagcg gatgtgacgc tgttctgcga tgtcctgtgt gatactgacc tgcaacgggt
      181 gttcactccg gacgaccgtg agcaggtgct ggccgtctat ggtccggtac atgcccggtt
      241 gctgcgtcag gcactggaac tgatcgctga tgcagagtcg gccagaaaaa agtagcccgc
      301 ccggaaattc gctttctgat gcgacttgcg ctccgtctgg ggcgcacctt atccgaactg
      361 cggcacagcc tgagtgcgag cgaggcgatg atgtggatgg agttcgacag ggtatccccg
      421 ct
//
LOCUS       DAZFWP020000570          422 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFWP020000570 DAZFWP020000000
VERSION     DAZFWP020000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.580"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>422
                     /locus_tag="ACHK0Z_RS27900"
                     /old_locus_tag="pgaptmp_005580"
     CDS             <1..>422
                     /locus_tag="ACHK0Z_RS27900"
                     /old_locus_tag="pgaptmp_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4505352.1"
                     /translation="FVNFLKANFNQEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAK
                     YSAIDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAY
                     SQLCKKLSLFKSIPSRLIKKSAYCSTENTITNKFDVVFS"
BASE COUNT          173 a           81 c           52 g          116 t
ORIGIN      
        1 ttcgttaatt ttttaaaggc aaacttcaac caagaaatat acccacagct atatactcct
       61 gaaatatttt atgaggcctg cagaaatctc cagtcatttt atgatcatca ggaaacgtca
      121 caaaacgcaa aatacagtgc aatagacaag aaaaaaagtc atcttaacca ggaaatcaaa
      181 aagcttatta aaaaaaatat tttccctgag ctatacaatg agcaatgcaa taaaatcccc
      241 tcctcatcga tagatgataa ccagggaata atgtggcaga actttaaaac tagcaatatt
      301 gcatattcac aactatgtaa aaaattatct ttatttaaaa gcatacccag tcgattaata
      361 aaaaaatcag cttactgctc aactgaaaac acaatcacca ataaatttga tgttgtcttt
      421 tc
//
LOCUS       DAZFWP020000571          421 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFWP020000571 DAZFWP020000000
VERSION     DAZFWP020000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.581"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            57..353
                     /locus_tag="ACHK0Z_RS27905"
                     /old_locus_tag="pgaptmp_005581"
     CDS             57..353
                     /locus_tag="ACHK0Z_RS27905"
                     /old_locus_tag="pgaptmp_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HGU4505353.1"
                     /translation="MDAANLSPSGRVLPLAATINGNNPVDETTGVMQPENGTNRTIRI
                     LGGVLISAGALAAIGAGIASLCVEDPSQKLGLGIAAGVLGGVTTLGSGLAMKYA"
BASE COUNT          138 a           77 c           99 g          107 t
ORIGIN      
        1 taatttggtt attgataacc agacaaatgt ctaaattaga caatggagaa aagttaatgg
       61 atgcagcaaa tttaagccct tctggcagag tattaccact ggcagccaca atcaatggaa
      121 ataatccagt ggatgagacg acaggagtaa tgcaaccaga aaatggaaca aatcgtacca
      181 ttagaatcct aggtggagtt ttgataagtg caggtgctct agccgctatc ggtgctggta
      241 tcgcatcact gtgtgttgaa gatccttcac aaaaattagg cttaggaatc gcggccggtg
      301 tattaggtgg cgtaacaact cttggcagcg gattagcgat gaaatatgcc taatcatcgc
      361 atcgcataaa taaaatagag aggtcatgga tgcattatgc cattacctct ctcatgaaaa
      421 g
//
LOCUS       DAZFWP020000572          420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFWP020000572 DAZFWP020000000
VERSION     DAZFWP020000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.582"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHK0Z_RS27910"
                     /old_locus_tag="pgaptmp_005582"
     CDS             complement(<1..176)
                     /locus_tag="ACHK0Z_RS27910"
                     /old_locus_tag="pgaptmp_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4505354.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVV"
     gene            complement(234..>420)
                     /locus_tag="ACHK0Z_RS27915"
                     /old_locus_tag="pgaptmp_005583"
     CDS             complement(234..>420)
                     /locus_tag="ACHK0Z_RS27915"
                     /old_locus_tag="pgaptmp_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4505355.1"
                     /translation="GQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEH
                     KKANAFAGSALSHAALP"
BASE COUNT           97 a          121 c          100 g          102 t
ORIGIN      
        1 tcaacaaccg gggagacata ggccgccacc ggcgtttttc cggtaatttt gtccagcatc
       61 acctcttcgg tatggaaatt caccgtacgg cggaaaaaca gctccagaaa cagcgcacga
      121 aatttcactt tttgttcggt ataaccgagt aactggcggg tcgtaaacaa tcccataaat
      181 cagttccttt cattcagaaa tcagtcaggc caccgcggtg gcctgataac gtgttacggc
      241 agcgccgcgt gactcagggc actgccggca aaggcatttg cctttttgtg ttcatccaca
      301 ctttcaggcc agcggattgc ctccgtcgca aaggtccccg acttgtaata ggtcagtacc
      361 gtctctgtgc cttcaagcgg cagtaccagt atgccaactg cactaccggc tttctgtccg
//
LOCUS       DAZFWP020000573          419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFWP020000573 DAZFWP020000000
VERSION     DAZFWP020000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.583"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          105 a           64 c           96 g          154 t
ORIGIN      
        1 agtgcacacg taatgatatc tgttgatacc atgtggttaa gtgtttccct tcctggtcat
       61 gcgtaatgat taaatgcttt tgtgacacca ttcttccggt ctgaatattt atcccgttct
      121 tttttatttg tgcagtgata ctgtcggaag agaattcagt aatgtgaggt gtcctggtca
      181 ggataatatc tgctgagata ttattagttg agtgtgtttt tactgagcaa aagctggctt
      241 ttaattcctg tactgaaaca ctgtcgctcg tccggggtta actgaaactg gatgatgatg
      301 taaaatgaag gctgtttttg tgtttgtcga agaagtgtta ttttaatcca ttctcatcat
      361 tggctggcga ttatacagaa gtatttgaag aaaaaatggt gcattttatc tgtgtcagc
//
LOCUS       DAZFWP020000574          417 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFWP020000574 DAZFWP020000000
VERSION     DAZFWP020000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.584"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            123..383
                     /gene="iraP"
                     /locus_tag="ACHK0Z_RS27920"
                     /old_locus_tag="pgaptmp_005584"
     CDS             123..383
                     /gene="iraP"
                     /locus_tag="ACHK0Z_RS27920"
                     /old_locus_tag="pgaptmp_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU4505356.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          141 a           75 c           91 g          110 t
ORIGIN      
        1 taatcaaaca aaaaatattt gcgcaaagta tttcctttgt cataaaaata atacttccag
       61 acactatgaa gttgtgaaac ataatgttaa cttctccata ctttggataa ggaaatacag
      121 acatgaaaaa tctcattgct gagttgttat ttaagcttgc ccaaaaagaa gaagagtcga
      181 aagaactgtg tgcgcaggta gaagctttgg agattatcgt cactgcaatg cttcgcaata
      241 tggcgcaaaa tgaccaacag cggttgattg atcaggtaga gggggcgctg tacgaggtaa
      301 agcccgatgc cagcattcct gacgacgata cggagctgct gcgcgattac gtaaagaagt
      361 tattgaagca tcctcgtcag taaaaagtta atcttttcaa cagctgtcat aaagttg
//
LOCUS       DAZFWP020000575          414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFWP020000575 DAZFWP020000000
VERSION     DAZFWP020000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.585"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..75
                     /locus_tag="ACHK0Z_RS27925"
                     /old_locus_tag="pgaptmp_005585"
     CDS             <1..75
                     /locus_tag="ACHK0Z_RS27925"
                     /old_locus_tag="pgaptmp_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505357.1"
                     /translation="EGIRITDIDTSGIFDSDDMTIKAA"
     gene            148..312
                     /locus_tag="ACHK0Z_RS27930"
                     /old_locus_tag="pgaptmp_005586"
     CDS             148..312
                     /locus_tag="ACHK0Z_RS27930"
                     /old_locus_tag="pgaptmp_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4505358.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            281..>414
                     /gene="kil"
                     /locus_tag="ACHK0Z_RS27935"
                     /old_locus_tag="pgaptmp_005587"
     CDS             281..>414
                     /gene="kil"
                     /locus_tag="ACHK0Z_RS27935"
                     /old_locus_tag="pgaptmp_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4505359.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     "
BASE COUNT          132 a           88 c           87 g          107 t
ORIGIN      
        1 gaaggaataa gaattaccga tatcgataca tcaggaatat ttgattcaga tgatatgact
       61 atcaaggccg cctgagcgcg gcgttaccgc ataccaatta cgcttcactc gaggcgtttt
      121 tcgttatgta taaataagga gcacaccatg caatatgcca ttgcagggtg gcctgttgct
      181 ggctgccctt ccgaatcttt acttgaacga atcacccgta aattacgtga cggatggaaa
      241 cgccttatcg acatacttaa tcagccagga gtcccaaaaa atggatcaaa caattatggc
      301 tatccagact aaattcacta tcgccacttt tattggcgat gaaaagatgt ttcgtgaggc
      361 cgtcgacgct tataaaaaat ggatattaat actgaaactg agatcaagca aaag
//
LOCUS       DAZFWP020000576          407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFWP020000576 DAZFWP020000000
VERSION     DAZFWP020000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.586"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>407)
                     /locus_tag="ACHK0Z_RS27940"
                     /old_locus_tag="pgaptmp_005588"
     CDS             complement(<1..>407)
                     /locus_tag="ACHK0Z_RS27940"
                     /old_locus_tag="pgaptmp_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGU4505360.1"
                     /translation="EKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLSGLIEL
                     SIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHSAIVKE
                     SSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQI"
BASE COUNT          127 a           98 c           54 g          128 t
ORIGIN      
        1 tttatctgca gcgaacggct atgtaagtca gagcgagtgc ctggaacaaa gtccattcca
       61 atacgtacag ccccctgaat ataatcacag atattgctag attcttttac tatagcacta
      121 tgtaaaatgc tacttacacc agacaatgca ttataaccag gtacagctcc catcgccata
      181 ttccaaattg tacctaaaaa atgtaacaca gctctcccat caggagaaag agaagactta
      241 tcacgatgat ttgtactatc attaatatcg atacttaact caatcaatcc acttaagcct
      301 tcttgaataa ccttatcaaa cccccaaata aactgctgcc cgccattcat atgggatact
      361 tcttttttga acttttctat tgcctctttt tgtttattat tcttttc
//
LOCUS       DAZFWP020000577          399 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFWP020000577 DAZFWP020000000
VERSION     DAZFWP020000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.587"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..184
                     /locus_tag="ACHK0Z_RS27945"
                     /old_locus_tag="pgaptmp_005589"
     CDS             <1..184
                     /locus_tag="ACHK0Z_RS27945"
                     /old_locus_tag="pgaptmp_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGU4505361.1"
                     /translation="ADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLE
                     IKHSQPKNRASGKRVS"
BASE COUNT          125 a           84 c           75 g          115 t
ORIGIN      
        1 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt ttcaacatcg
       61 actggtaaac agaaattgtt atttagataa atatggcgat gcacgaatca atgcttctta
      121 tacacaatta gaaataaaac actcacaacc caaaaataga gcatccggca aaagagtgag
      181 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      241 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      301 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      361 tgacttttct taccctcaga gttctgcccc ctgtcaact
//
LOCUS       DAZFWP020000578          397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFWP020000578 DAZFWP020000000
VERSION     DAZFWP020000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.588"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..302
                     /locus_tag="ACHK0Z_RS27950"
                     /old_locus_tag="pgaptmp_005590"
     CDS             <1..302
                     /locus_tag="ACHK0Z_RS27950"
                     /old_locus_tag="pgaptmp_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU4505362.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>397
                     /locus_tag="ACHK0Z_RS27955"
                     /old_locus_tag="pgaptmp_005591"
     CDS             299..>397
                     /locus_tag="ACHK0Z_RS27955"
                     /old_locus_tag="pgaptmp_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505363.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGT"
BASE COUNT           94 a           86 c          137 g           80 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtaca
//
LOCUS       DAZFWP020000579          396 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFWP020000579 DAZFWP020000000
VERSION     DAZFWP020000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.589"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>396
                     /locus_tag="ACHK0Z_RS27960"
                     /old_locus_tag="pgaptmp_005592"
     CDS             <1..>396
                     /locus_tag="ACHK0Z_RS27960"
                     /old_locus_tag="pgaptmp_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4505364.1"
                     /translation="GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDFVACGDMTIV
                     RRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWK
                     LGGAIKTTERKLAEGVLVHGGQPLMAWCVG"
BASE COUNT           78 a           84 c          153 g           81 t
ORIGIN      
        1 tggccgtgac agggaaaccc gcgaatggct gggctggggc catgcctggg cgcatgaaac
       61 cgcggtggtc agacggaaga gtgaggcatc ccgttttcag gattttgtgg cctgtggaga
      121 catgacgatt gtccgtcggg tcggggatga cacggcggaa gtggcggagt atgtgcgtcg
      181 tattcatgag gctgagttac tggagcatat cggtattgac ccgtcaggtg tggggcagat
      241 tctggattca ctggcggaag ccgggatccc cgacggaatt gttgtgggga taagccaggg
      301 ctggaagctg ggcggggcca taaaaaccac cgagcgcaaa ctggctgagg gagtactggt
      361 gcatggtggt cagccactga tggcctggtg cgttgg
//
LOCUS       DAZFWP020000580          395 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFWP020000580 DAZFWP020000000
VERSION     DAZFWP020000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.590"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>395
                     /locus_tag="ACHK0Z_RS27965"
                     /old_locus_tag="pgaptmp_005593"
     CDS             <1..>395
                     /locus_tag="ACHK0Z_RS27965"
                     /old_locus_tag="pgaptmp_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4505365.1"
                     /translation="AEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARD
                     VRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAR
                     EAGEEAFRGFMSKHANVEIGLALRSDRWAG"
BASE COUNT           86 a          101 c          119 g           89 t
ORIGIN      
        1 gcggaagaca tgctacgtga agccatcggc gggcttgcct cccgtccgga aggatttgtg
       61 gtgtacacaa ccacccagtc gaatgaaccg cctgccgggg tgttcagaca gaaactgcag
      121 tacgcccggg atgtccgtga cggcaaaatt catgatccgc actttctgcc ggtgatattt
      181 gaacaccctc ctgaaatggt ggaaagcggg gctcacctgc tgatggaaaa cctcgccatg
      241 gtcaatccga atctcggtta ttcggtggat gaggcttttc tgtaccggga gtaccgtaaa
      301 gcccgggagg ctggtgagga agcatttcgt ggcttcatgt caaaacatgc caatgtggaa
      361 attggtcttg ccctgcgttc tgaccgctgg gcggg
//
LOCUS       DAZFWP020000581          390 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFWP020000581 DAZFWP020000000
VERSION     DAZFWP020000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.591"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..251)
                     /locus_tag="ACHK0Z_RS27970"
                     /old_locus_tag="pgaptmp_005594"
     CDS             complement(<1..251)
                     /locus_tag="ACHK0Z_RS27970"
                     /old_locus_tag="pgaptmp_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4505366.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTL"
     gene            293..>390
                     /locus_tag="ACHK0Z_RS27975"
                     /old_locus_tag="pgaptmp_005595"
     CDS             293..>390
                     /locus_tag="ACHK0Z_RS27975"
                     /old_locus_tag="pgaptmp_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505367.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKG"
BASE COUNT          115 a          117 c           80 g           78 t
ORIGIN      
        1 cagagcgtcc agagattcgt ctcctcatta ccaccaccga actgaagtgc aatgcggtga
       61 tcgagttcac tgtcacagag gtcaaccaca cgaccacaga gacagcactg cccggcatcc
      121 ctcagccaga tatgacgctt gagggaaacc cgggcactgc cactgacacg acgctgttcc
      181 cccttcagaa tattcacccg tcgggtattc agtgttttga ttctgctctg gagtgtacga
      241 agctcagcca tgtaaaatcc ccgtcatata acttgtcacc agaggaaaga aaatgtcatc
      301 gaaaaaccgg acccgcagaa ccacaacccg caatatccgt ttccccaatc acataattga
      361 acaaatcaac atagcccttg agcataaagg
//
LOCUS       DAZFWP020000582          388 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFWP020000582 DAZFWP020000000
VERSION     DAZFWP020000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.592"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..380)
                     /locus_tag="ACHK0Z_RS27980"
                     /old_locus_tag="pgaptmp_005596"
     CDS             complement(<1..380)
                     /locus_tag="ACHK0Z_RS27980"
                     /old_locus_tag="pgaptmp_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HGU4505368.1"
                     /translation="MNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREVSGN
                     GIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEG
                     DVSVDDVQFTMLNKDVYPVIENNNG"
BASE COUNT          120 a           90 c           66 g          112 t
ORIGIN      
        1 ccattattat tttctattac cggataaaca tctttattta gcatcgtaaa ttgaacatca
       61 tcaacagaca catcaccttc tggtaaagca gtctgcatta cccataagat catgagtttt
      121 atcatatctt ccggtatcag ccggctttcg ctatacggga ataactcctg gcaccactga
      181 atgaactgtt cttctgatat ccaaaaacgc ccctcatagc ctgagcatgt taacgtgaag
      241 cggataccat taccgctgac ttcgcgaaaa tatgcatggc aagcatgccc ttcaggcgta
      301 atatagaggc gaccggttcc tatgactgac aacaatggca agtaaaaatc attaattttc
      361 atattcaatt gagaattcaa taggttca
//
LOCUS       DAZFWP020000583          388 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFWP020000583 DAZFWP020000000
VERSION     DAZFWP020000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.593"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(44..>388)
                     /locus_tag="ACHK0Z_RS27985"
                     /old_locus_tag="pgaptmp_005597"
     CDS             complement(44..>388)
                     /locus_tag="ACHK0Z_RS27985"
                     /old_locus_tag="pgaptmp_005597"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4505369.1"
                     /translation="TGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALIISFWL
                     DVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAVLITIL
                     VAIIIWGVLLLS"
BASE COUNT          130 a           88 c           63 g          107 t
ORIGIN      
        1 taaatattaa ccaggtaact gtgaataatt tagatgttgg gcgttatgaa agtaataaaa
       61 ctccccatat aatgatagca actaaaatcg taatcaacac tgcggctgaa cccatatctt
      121 ttgctctgcc tgataattcg tgatgttctg aaccaattct gtcaacaact gcttcaattg
      181 cactgttaat tatttcaaca ataattacca tcattgccga accaatgagt aatactcgag
      241 taacagagtc aacatccagc caaaaggata ttatcagtgc tgacagaacc agaatgcact
      301 cctgacgaaa ggctgcttca tagcgccatg ccgcacataa gcctttccat gaataacccg
      361 ctgctttaat aattctggta acaccagt
//
LOCUS       DAZFWP020000584          387 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFWP020000584 DAZFWP020000000
VERSION     DAZFWP020000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.594"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>387
                     /locus_tag="ACHK0Z_RS27990"
                     /old_locus_tag="pgaptmp_005598"
     CDS             <1..>387
                     /locus_tag="ACHK0Z_RS27990"
                     /old_locus_tag="pgaptmp_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4505370.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVA"
BASE COUNT           73 a          119 c          119 g           76 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggca ttcagccttc tcccgcacag gcacagaaca
      121 ttcgggagaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgttgccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctaatg acagcacttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccgcgctca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tgttgcctct gttgccc
//
LOCUS       DAZFWP020000585          387 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFWP020000585 DAZFWP020000000
VERSION     DAZFWP020000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.595"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(30..317)
                     /gene="nleC"
                     /locus_tag="ACHK0Z_RS27995"
                     /old_locus_tag="pgaptmp_005599"
                     /pseudo
     CDS             complement(30..317)
                     /gene="nleC"
                     /locus_tag="ACHK0Z_RS27995"
                     /old_locus_tag="pgaptmp_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
BASE COUNT          119 a           76 c           80 g          112 t
ORIGIN      
        1 ccatagctta cggcatcacg aaaagtttat gagcggctca aagcatcaag tatagtatta
       61 ttaatcatat cgaccgtatg ctgatcaatg agtctgccac gactgtcatg gacggcacga
      121 attacacttt cgtatacgtt gcttaaatct gctgcggaaa gtggtattcg cttgcctata
      181 tccaaaacgt aatccgcata ggcattttca gcacgattag gagcaatgag agcagagtat
      241 cctgccgggg cggaaaagtt gaagttggac tgtaatgagg gaattttcat gttgatacct
      301 aataccatgc aaatcattta ttacaacaga ataaacttta tcaaattcat ttaccatgaa
      361 ctgcatgtat actgacttgt gttcatg
//
LOCUS       DAZFWP020000586          387 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFWP020000586 DAZFWP020000000
VERSION     DAZFWP020000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.596"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>387
                     /locus_tag="ACHK0Z_RS28000"
                     /old_locus_tag="pgaptmp_005600"
     CDS             <1..>387
                     /locus_tag="ACHK0Z_RS28000"
                     /old_locus_tag="pgaptmp_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HGU4505371.1"
                     /translation="DNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTP
                     RVVKGVRFSLRLTSGSGQDSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGE
                     PATTTFRINAPAKPATIELTPGYFQIT"
BASE COUNT           87 a          120 c          120 g           60 t
ORIGIN      
        1 ggataacggg gccagctttg agccgcagtc aggcaccctg aacagcgtta ttccaccggc
       61 agtgcagcac ctgacggtgg aggtgagcgc cgctgacggc cagtatctgg cacaggcgaa
      121 atgggacacg ccgcgggtgg tgaagggcgt gcgcttcagt ctgcgcctga ccagtggaag
      181 cggtcaggac agccgtctgg tgaccaccgc catcaccgcg gatacagagc accgtttcag
      241 cggtctgccg ccgggggaat acaccctgac ggtcagggca attaacagtt atggccagca
      301 gggcgaaccg gccaccacca ccttccggat taacgcgcca gcaaaacccg ccaccattga
      361 actgacaccg ggctattttc agataac
//
LOCUS       DAZFWP020000587          386 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFWP020000587 DAZFWP020000000
VERSION     DAZFWP020000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.597"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..73
                     /locus_tag="ACHK0Z_RS28005"
                     /old_locus_tag="pgaptmp_005601"
                     /pseudo
     CDS             1..73
                     /locus_tag="ACHK0Z_RS28005"
                     /old_locus_tag="pgaptmp_005601"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
     gene            52..198
                     /locus_tag="ACHK0Z_RS28010"
                     /old_locus_tag="pgaptmp_005602"
     CDS             52..198
                     /locus_tag="ACHK0Z_RS28010"
                     /old_locus_tag="pgaptmp_005602"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGU4505372.1"
                     /translation="MTSGVDNATGPRLAEAAERDYFILRERLMAMQKQLEGAQEYIRT
                     QCIP"
BASE COUNT          113 a           81 c           95 g           97 t
ORIGIN      
        1 aagcgcctgc gcatcaacgc caactgtcca ggctccttgc gtaaagcccc catcacctcc
       61 ggcgtggata atgcaaccgg tccccgactg gcagaagccg ctgaacggga ttatttcatc
      121 ctcagagaac ggctgatggc aatgcagaag caactggaag gagcacagga atatatccgt
      181 acccagtgta taccgtgatg ttttgttacg aaggtgttac tggtaacgtt aaggtaattt
      241 aacaaagagt cagttccgga ctttatagtg tgctcagttc atggccaaaa acgatttctg
      301 tgataaatat tttgaatatt atttacaggt aaatggagtg gggcgcatgg atagaaatat
      361 tacaatagag tatgaagtat atgccc
//
LOCUS       DAZFWP020000588          383 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFWP020000588 DAZFWP020000000
VERSION     DAZFWP020000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.598"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>383)
                     /locus_tag="ACHK0Z_RS28015"
                     /old_locus_tag="pgaptmp_005603"
     CDS             complement(<1..>383)
                     /locus_tag="ACHK0Z_RS28015"
                     /old_locus_tag="pgaptmp_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505373.1"
                     /translation="AMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPA
                     FSLTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKA
                     FPRNNSYASGTVTVTVYDDQGFDRQ"
BASE COUNT           75 a          112 c           95 g          101 t
ORIGIN      
        1 atctgccggt cgaagccctg gtcatcgtaa actgtgacgg ttaccgtccc gctggcataa
       61 ctgttattcc ggggaaaggc tttccccacc gtcttcacca ggtcgccttc tatctggttt
      121 gcagacagtt tccctctgat gacacagttc tcgttaatgg tgacattatt gagcgtgccg
      181 gtattcgcgg taattgctcc actgatatcc gcgttcctgg ctgtcagctt cccttccggc
      241 gtcagggaaa atgccggggg attgccggac gaggtgatac tcaccgcaaa cagtcgcttc
      301 aggaacacat cgttcatgaa caactgattc ccctgcgcca caaataacgg cgtgctgttg
      361 ccgctctccg gatttatcat cgc
//
LOCUS       DAZFWP020000589          382 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFWP020000589 DAZFWP020000000
VERSION     DAZFWP020000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.599"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>382
                     /locus_tag="ACHK0Z_RS28020"
                     /old_locus_tag="pgaptmp_005604"
     CDS             <1..>382
                     /locus_tag="ACHK0Z_RS28020"
                     /old_locus_tag="pgaptmp_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGU4505374.1"
                     /translation="FQRELDLADKAILWALISASTKEGRKACSLSYFACKAAEAELGL
                     AYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADC
                     IETVNKIKDKNKKIIDINSVNICSD"
BASE COUNT          145 a           61 c           72 g          104 t
ORIGIN      
        1 atttcagaga gagttagatt tggcagacaa agcaatatta tgggcactaa tatcagccag
       61 tactaaagag ggacgaaaag cgtgttcact aagctatttt gcctgtaagg ccgcagaggc
      121 tgaactcgga ctggcatata tggctgcgaa tgataacaag gaatttctca catccttatc
      181 taatataatg aggtacaaaa tagatgctgg actttccgaa tcctatacat gctatttatt
      241 aagtaaggga aagatcataa gaccatatct gaaaaatcta aatccactcc aacttgccgc
      301 tgattgcatt gaaacagtaa ataaaataaa agataagaat aaaaaaatca ttgatataaa
      361 ttcagttaat atttgtagtg at
//
LOCUS       DAZFWP020000590          380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFWP020000590 DAZFWP020000000
VERSION     DAZFWP020000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.600"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..259)
                     /locus_tag="ACHK0Z_RS28025"
                     /old_locus_tag="pgaptmp_005605"
     CDS             complement(<1..259)
                     /locus_tag="ACHK0Z_RS28025"
                     /old_locus_tag="pgaptmp_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HGU4505375.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNEPSKIDKMLEINSNRDLLGMFFLSLKEDSVLL"
BASE COUNT          126 a           59 c           71 g          124 t
ORIGIN      
        1 tcaaaagtac gctatcttct tttaatgata gaaaaaacat accgagtaga tctcgattgc
       61 tatttatttc tagcatttta tcaatttttg aaggttcatt attgaataaa taacaataaa
      121 aaaacaggac ctcagattga ggcgcacact ccagtatcca ttcatgacca tcgtgatcat
      181 ggaaagagag ggcgttagtt tgttcttcaa gagttattcc gaacgtatgt gcaagatctg
      241 tgcgaaattg ttcattcata ttgtagtcct gtcattcttt caatgagggt atgcttaaac
      301 gtaattaact cgtgagttct ggcacaaata ataagacgaa aatataagat gcgagtaata
      361 gtgttgcata tttatgatag
//
LOCUS       DAZFWP020000591          371 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFWP020000591 DAZFWP020000000
VERSION     DAZFWP020000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.601"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>371
                     /locus_tag="ACHK0Z_RS28030"
                     /old_locus_tag="pgaptmp_005606"
     CDS             <1..>371
                     /locus_tag="ACHK0Z_RS28030"
                     /old_locus_tag="pgaptmp_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HGU4505376.1"
                     /translation="LRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTW
                     QGFVYVAFIIDVFAGYIVGWQVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYV
                     SLAYTQRLKEAGLLASTGSTG"
BASE COUNT           81 a           94 c          113 g           83 t
ORIGIN      
        1 ctccggggta aaaaggtccg cactaccgtc agccggaaag ccgttgccgc atgcgaccgc
       61 gtaaaccgtc agttcgtggc agaacgtcca gaccagttgt gggtggctga ttttacctgg
      121 gtaagcacat ggcagggctt cgtttatgtg gcgttcatca ttgatgtgtt cgccggatac
      181 atcgtgggat ggcaggtctc atcatccatg gaaacaacat tcgtgctgga tgcactggag
      241 caggcgttgt gggcccgtcg gccgtccggc acaatccatc acagtgataa aggttctcag
      301 tatgtatcgc tggcctacac gcagcggctt aaggaagccg gattactggc atcaacaggg
      361 agtactggcg a
//
LOCUS       DAZFWP020000592          366 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFWP020000592 DAZFWP020000000
VERSION     DAZFWP020000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.602"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..290
                     /locus_tag="ACHK0Z_RS28035"
                     /old_locus_tag="pgaptmp_005607"
     CDS             <1..290
                     /locus_tag="ACHK0Z_RS28035"
                     /old_locus_tag="pgaptmp_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HGU4505377.1"
                     /translation="IRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGIA
                     LALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
BASE COUNT           70 a           80 c          112 g          104 t
ORIGIN      
        1 tcattcgctt ccatatcccg ctgtgggtgc gtcgtacagt caccatgttg ccgtcattta
       61 ttgtcattct gatgggatta gatccgacac ggattctggt tatgagtcag gtgctgttaa
      121 gttttggtat cgccctggcg ctggttccac tgctgatttt caccagtgac agcaagttga
      181 tgggcgatct ggtgaacagc aaacgcgtaa aacagacagg ctgggtgatt gtagtgctgg
      241 tcgtggcgct gaatatctgg ttgttggtgg ggacggcgct gggattgtag ttgaatgagc
      301 gtcgcatctg gcactattgg cggatgcggc gtaaacgcct tatccgccct acgcgttaaa
      361 gagccg
//
LOCUS       DAZFWP020000593          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFWP020000593 DAZFWP020000000
VERSION     DAZFWP020000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.603"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            88..>365
                     /locus_tag="ACHK0Z_RS28040"
                     /old_locus_tag="pgaptmp_005608"
     CDS             88..>365
                     /locus_tag="ACHK0Z_RS28040"
                     /old_locus_tag="pgaptmp_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HGU4505378.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
BASE COUNT           95 a           81 c          104 g           85 t
ORIGIN      
        1 gggtgagctt acggagtggt cagtgtgact ttctctgtgg gcagatcgct cccgggcgtt
       61 ggctcaccca cccaaaggaa cgtcacgatg tttggaatct tcaaaaagaa aacccgcaga
      121 gcggcagcgg aaattaaaaa gtttgagaaa cgcgatctgg cacaggcggt gattaacgct
      181 gcatacctgg tggcctatgc agatggtgaa tgcgaggcat ccgagaaagc gaagatcgaa
      241 caggtcttac gtaatcagcc tgcgttgtct gcgtttacct cggaaattaa tgcgattagc
      301 gcaactatta tcggtcagct ggataccaat tttaaaattg gtcgtcgtgc cgcgttacgc
      361 gagat
//
LOCUS       DAZFWP020000594          362 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFWP020000594 DAZFWP020000000
VERSION     DAZFWP020000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.604"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>362
                     /locus_tag="ACHK0Z_RS28045"
                     /old_locus_tag="pgaptmp_005609"
     CDS             <1..>362
                     /locus_tag="ACHK0Z_RS28045"
                     /old_locus_tag="pgaptmp_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4505379.1"
                     /translation="DEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQY
                     FKLQLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQA
                     IETLSAIGLSYEKYKTAN"
BASE COUNT          132 a           60 c           70 g          100 t
ORIGIN      
        1 ggatgaagca gtctgggtca aacaagcact ggaaaaaaat caaccaggaa agccagtacg
       61 ggtgataaat cctgatgatg aatcgatacg aattttttca tggcttgctg ataatttccc
      121 tgatttacaa tattttaaac ttcaactatt agatgccagt aatctaagat tgacagtgag
      181 taagcaacgg aacgctatca cacagcaact aatcgataat cttattaaag gattactgca
      241 aactatgcca tatgccagca atataagtat tgcggtatta gatgataatg tattggaaag
      301 tcaggctatt gaaacgttgt cagcgatagg tctttcttat gaaaaatata aaacagctaa
      361 ca
//
LOCUS       DAZFWP020000595          360 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFWP020000595 DAZFWP020000000
VERSION     DAZFWP020000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.605"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            122..197
                     /locus_tag="ACHK0Z_RS28050"
                     /old_locus_tag="pgaptmp_005610"
     tRNA            122..197
                     /locus_tag="ACHK0Z_RS28050"
                     /old_locus_tag="pgaptmp_005610"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT          102 a           82 c           83 g           93 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaacga aagttgttcg tgagtctctc aaattttcgc aactctgaag
//
LOCUS       DAZFWP020000596          356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFWP020000596 DAZFWP020000000
VERSION     DAZFWP020000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.606"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..286)
                     /locus_tag="ACHK0Z_RS28055"
                     /old_locus_tag="pgaptmp_005611"
     CDS             complement(<1..286)
                     /locus_tag="ACHK0Z_RS28055"
                     /old_locus_tag="pgaptmp_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4505380.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALIT"
BASE COUNT          115 a           74 c           71 g           96 t
ORIGIN      
        1 tggtaattaa agccgcagcc aacgcaggat tgttgggttc cagcaacagg taaagcggaa
       61 tgtcagtcag acgagtcctc gtcagcttat gctgactttc cagatattga cttacgacgg
      121 attcgcttat atcgacaatt ttaactttgc cataatgcat aaggaaaagc tccctgatgc
      181 atttggtggc cagaacgact gagcctggct taagtgacaa cgtatccttt tcaagaaaaa
      241 tattaattgg ggagcaaacc atgataactg aacagacaac agccattata attttacttt
      301 cattagcaat tggttagctc aattatagcc ccaaaaggta aattatcatc aacaca
//
LOCUS       DAZFWP020000597          351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFWP020000597 DAZFWP020000000
VERSION     DAZFWP020000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.607"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            100..351
                     /locus_tag="ACHK0Z_RS28060"
                     /old_locus_tag="pgaptmp_005612"
                     /pseudo
     CDS             100..351
                     /locus_tag="ACHK0Z_RS28060"
                     /old_locus_tag="pgaptmp_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          128 a           52 c           54 g          117 t
ORIGIN      
        1 gaacatataa gtattattgc tttgtatcat cgtaaatatg tctttcagac atgtattgtt
       61 tattttgtta ctaacaagta aaaatattac aggttattta tgaaagtatt tcacaatata
      121 tttaagcact tctcttcaaa ccatcaagat aagcattcag ataaagttaa cagtcatcaa
      181 caccatggca aggttgataa aaaatatcga tcaaacacag tttaagttaa tatgctgaat
      241 gacttttcgc aaatagataa cgatttaggc ctgaatatca tctatttcct gcaacatggt
      301 cactggaggg taaatgaaca tagtcttcag atggagaaaa tatggtttta c
//
LOCUS       DAZFWP020000598          350 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFWP020000598 DAZFWP020000000
VERSION     DAZFWP020000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.608"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>350
                     /locus_tag="ACHK0Z_RS28065"
                     /old_locus_tag="pgaptmp_005613"
     CDS             <1..>350
                     /locus_tag="ACHK0Z_RS28065"
                     /old_locus_tag="pgaptmp_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505381.1"
                     /translation="LDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNY
                     VKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLS
                     NEDKEKIISDLDKV"
BASE COUNT          128 a           66 c           57 g           99 t
ORIGIN      
        1 cactcgacat caggaacaca tatcgcccgg ataatgagat ggcattttac tatcatattc
       61 attcttttct cactcagata ccggataatg aggatgatat atatcatgct gcaaggacat
      121 atctgcgaaa ttacgttaag ttatgtttat ccggatacac gccagcgaat gcgcatttca
      181 aagatatctt tgatggcgta tatgaattca ttcgtaatat tcgcaaaaac agtacaccag
      241 gaaaaacaaa acttatcgca actatcaaca catgcaaaga aacctgtaaa catctgcttt
      301 atttaagtaa tgaagacaag gaaaaaataa tttctgactt agataaagtt
//
LOCUS       DAZFWP020000599          349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFWP020000599 DAZFWP020000000
VERSION     DAZFWP020000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.609"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>349
                     /gene="sepL"
                     /locus_tag="ACHK0Z_RS28070"
                     /old_locus_tag="pgaptmp_005614"
     CDS             <1..>349
                     /gene="sepL"
                     /locus_tag="ACHK0Z_RS28070"
                     /old_locus_tag="pgaptmp_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HGU4505382.1"
                     /translation="KFVKIELKKKYAKLLLDLLGEDDWELALLSWLGVGELNQEGIQK
                     IKKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVK
                     TSSIISDLCRVIIF"
BASE COUNT          107 a           43 c           85 g          114 t
ORIGIN      
        1 taagtttgtt aagattgaat taaaaaagaa atacgccaaa ttattattag atttattagg
       61 cgaagatgat tgggagttgg ctctgctttc ctggttaggg gtgggtgagt taaatcagga
      121 aggtatccag aagatcaaaa agctttatga aaaggccaag gatgaggatt ctgaaaaggg
      181 agcctcttta cttgactggt ttatggagat taaggatctt ccagagcgtg agaagcactt
      241 aaaggttatt attagggcgc tatcgttcga tctgtcttat atgtcttctt ttgaagataa
      301 ggtgaaaaca tcttcaatta ttagcgattt atgccgggta atcattttt
//
LOCUS       DAZFWP020000600          347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFWP020000600 DAZFWP020000000
VERSION     DAZFWP020000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.610"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..345
                     /locus_tag="ACHK0Z_RS28075"
                     /old_locus_tag="pgaptmp_005615"
     CDS             <1..345
                     /locus_tag="ACHK0Z_RS28075"
                     /old_locus_tag="pgaptmp_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4505383.1"
                     /translation="GNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGTFGAFDNEWH
                     TLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRNATYPVLIDS
                     IAVEVNSTDTAA"
BASE COUNT           97 a           83 c           95 g           72 t
ORIGIN      
        1 gggaataacc tgctggcagc gttctacatc cagacagatg caaaagacct gaatgtgatg
       61 taccacaatg cgaaagtggc gacaaacaac ctgaaactgg gaacctttgg cgcatttgat
      121 aacgaatggc atacgctggc tttccgcttt gccgggaata acagccttca ggtgacgccg
      181 gttattgatg gtcaggatgg cacaccgttc acgctgacgc agtcaccggt cagtgcattt
      241 gcggcggata aactgcatgt gacagacatt accaggaatg cgacttaccc ggtactgata
      301 gacagcattg cggtggaagt gaacagcaca gacactgcgg catgata
//
LOCUS       DAZFWP020000601          344 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFWP020000601 DAZFWP020000000
VERSION     DAZFWP020000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.611"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            128..334
                     /locus_tag="ACHK0Z_RS28080"
                     /old_locus_tag="pgaptmp_005616"
     CDS             128..334
                     /locus_tag="ACHK0Z_RS28080"
                     /old_locus_tag="pgaptmp_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU4505384.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT           96 a           70 c           85 g           93 t
ORIGIN      
        1 caataagcaa aaataagtgg tcggatgagt agtaaaatta cacaaaaggc ggtactattt
       61 tcatcagata aacaaattca tttttccggt gccattcacc gggtagatac aaaaggggga
      121 gaaaagtatg gaactctaca gagaatatcc tgcatggctt atctttttac gccgtactta
      181 tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag accgcgcccg
      241 cttttacagc tacctgcatc gcgtctggtc gaaaaccagc gataaaccgg tgtggatgga
      301 tcaggccgaa aaggcaactg gtgattttta ttgattgctg agtt
//
LOCUS       DAZFWP020000602          344 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFWP020000602 DAZFWP020000000
VERSION     DAZFWP020000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.612"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>344)
                     /locus_tag="ACHK0Z_RS28085"
                     /old_locus_tag="pgaptmp_005617"
     CDS             complement(<1..>344)
                     /locus_tag="ACHK0Z_RS28085"
                     /old_locus_tag="pgaptmp_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HGU4505385.1"
                     /translation="TTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVA
                     FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQR
                     LKEAGLLASTGST"
BASE COUNT           78 a          105 c           88 g           73 t
ORIGIN      
        1 gtacttcctg ttgatgccag taatccggct tccttaagcc gctgtgtgta ggccagcgat
       61 acatactgag aacctttatc actgtgatgg accgtgccgg acggtcgacg ggcccataac
      121 gcctgctcca gtgcatccag cacgaatgtc gtctccatgg acgatgagac ccgccacccc
      181 acaatgtatc cggcaaacac atcaatgatg aacgccacat agacgaagcc ctgccatgtg
      241 ctgacgtaag taaaatcagc cacccacagc tggtcaggtc gttctgccac gaactgacgg
      301 tttacgcggt cgcctgcggc aacggctttc cggctgatgg tcgt
//
LOCUS       DAZFWP020000603          344 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFWP020000603 DAZFWP020000000
VERSION     DAZFWP020000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.613"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            50..343
                     /locus_tag="ACHK0Z_RS28090"
                     /old_locus_tag="pgaptmp_005618"
     CDS             50..343
                     /locus_tag="ACHK0Z_RS28090"
                     /old_locus_tag="pgaptmp_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HGU4505386.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
BASE COUNT          126 a           60 c           63 g           95 t
ORIGIN      
        1 tgggataaac attattttaa actccaatct tgcgtaagtg aaagttgata tgtcattagt
       61 aggatataat agtaacatca taataaccat cgcgctgata tcgttgctct ctgcatgcac
      121 cagagaaaat tcattaaagg agaatcaaaa taaaagaagt ggcgaatccc aagcgatagt
      181 agatgcaaga cagttcttta gcgaacaccc ggaatatata tcatcagcag aactggagca
      241 aaagctatat cgcgaatttg cacaggttac tacagttcca gcttataaag agatgagtat
      301 gtatcaattg cttatcattt cacaagatcg tttaactcat taaa
//
LOCUS       DAZFWP020000604          340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFWP020000604 DAZFWP020000000
VERSION     DAZFWP020000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.614"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>340
                     /gene="nleB2"
                     /locus_tag="ACHK0Z_RS28095"
                     /old_locus_tag="pgaptmp_005619"
     CDS             <1..>340
                     /gene="nleB2"
                     /locus_tag="ACHK0Z_RS28095"
                     /old_locus_tag="pgaptmp_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HGU4505387.1"
                     /translation="YAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIY
                     LDMDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSK
                     VDAHPYYDGLG"
BASE COUNT          108 a           45 c           75 g          112 t
ORIGIN      
        1 gtatgcaaag aaaagaaatc atgcatggtt tgatttcttc agaaatttag ccttattaaa
       61 agcaggagag atattcaggt gcacatataa tacaaagaat cacggtattt cattcgggga
      121 ggggtgtatc tatcttgata tggatatgat acttacaggt aagcttggta caatatatgc
      181 tcctgatgga atttcaatgc atgtggatcg tcgtaatgat agtgtaaata ttgaaaatag
      241 tgcaataatt gttaaccgta gtaatcatcc tgctctactt gagggacttt cttttatgca
      301 tagtaaagta gatgctcatc catattatga tggtttgggg
//
LOCUS       DAZFWP020000605          333 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFWP020000605 DAZFWP020000000
VERSION     DAZFWP020000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.615"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..>333
                     /locus_tag="ACHK0Z_RS28100"
                     /old_locus_tag="pgaptmp_005620"
     rRNA            1..>333
                     /locus_tag="ACHK0Z_RS28100"
                     /old_locus_tag="pgaptmp_005620"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           96 a           72 c          103 g           62 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgcgcgat acagggtgac agc
//
LOCUS       DAZFWP020000606          329 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFWP020000606 DAZFWP020000000
VERSION     DAZFWP020000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.616"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>329)
                     /locus_tag="ACHK0Z_RS28105"
                     /old_locus_tag="pgaptmp_005621"
     CDS             complement(<1..>329)
                     /locus_tag="ACHK0Z_RS28105"
                     /old_locus_tag="pgaptmp_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4505388.1"
                     /translation="TGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELT
                     DRWVVEQMSSLTAMTASFVLATPTETDGALFPGRIMLANTCMWTYRSDECGYTGGAVA
                     DEFDKPTT"
BASE COUNT           66 a          123 c           86 g           54 t
ORIGIN      
        1 gtggtgggtt tatcgaactc atccgccaca gccccgcccg tgtaaccaca ctcatcagag
       61 cggtaggtcc acatacaggt gttcgccagc atgatgcgac cgggaaacag cgccccgtcc
      121 gtctcggtcg gtgtggccag cacaaacgag gccgtcatgg ccgtcagcga tgacatctgc
      181 tccaccaccc accggtcagt cagctcctgc tccgggtcgg cctccggatt gcctgccaca
      241 aagttcaccg catccagaaa acgcgcatac acccggcggc ggaccaccgt ggcacccacc
      301 aggctctgca aatcctccgc catcccggt
//
LOCUS       DAZFWP020000607          327 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFWP020000607 DAZFWP020000000
VERSION     DAZFWP020000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.617"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..206)
                     /locus_tag="ACHK0Z_RS28110"
                     /old_locus_tag="pgaptmp_005622"
     CDS             complement(<1..206)
                     /locus_tag="ACHK0Z_RS28110"
                     /old_locus_tag="pgaptmp_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4505389.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFEL"
     gene            complement(206..>327)
                     /locus_tag="ACHK0Z_RS28115"
                     /old_locus_tag="pgaptmp_005623"
     CDS             complement(206..>327)
                     /locus_tag="ACHK0Z_RS28115"
                     /old_locus_tag="pgaptmp_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505390.1"
                     /translation="FLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSAEFEQVVN"
BASE COUNT           71 a          109 c           69 g           78 t
ORIGIN      
        1 ttcagttcaa aaccgctccc ctgaatggga tacggctgat actgtcgccc ctgccaggtg
       61 accggctcac ctttttcgtt ctgctcatta cagaaaaaat aacgttctcc accgacctct
      121 gtcaggtcga tttcccagag caccacgctg gccgactgct ccgcacgggt gcattcattc
      181 agtgtttcct gccggatatc ctgcatcagt tcaccacctg ttcaaactct gcgctgaact
      241 caacacgcag catactgacc cgcgacgacc attttgcgca ggtcaccttt atctgccgcc
      301 actcataagg cggcgtccac agaaagg
//
LOCUS       DAZFWP020000608          324 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFWP020000608 DAZFWP020000000
VERSION     DAZFWP020000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.618"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>324
                     /locus_tag="ACHK0Z_RS28120"
                     /old_locus_tag="pgaptmp_005624"
     CDS             <1..>324
                     /locus_tag="ACHK0Z_RS28120"
                     /old_locus_tag="pgaptmp_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4505391.1"
                     /translation="IDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQ
                     THVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDD
                     IISSCR"
BASE COUNT          104 a           51 c           61 g          108 t
ORIGIN      
        1 attgacaagc ttatcgaata taaagatgag tatgatactg ttttattata tgtctctgac
       61 catggagaat ctctgggaga aaatggatta tacttacatg gaacaccata taatgttgct
      121 ccagctgagc aaacgcatgt gcctcttatt acctggatgt ctccggggtt cgtttcctct
      181 aaaaaaattg atttgaattg tttgtcagaa catgctctaa acagaacagt aagtcatgat
      241 aatatatttt catcgttact ggggttatgg aatgtaaata cttcagttta taataaagaa
      301 gatgatatta tatcctcctg caga
//
LOCUS       DAZFWP020000609          322 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFWP020000609 DAZFWP020000000
VERSION     DAZFWP020000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.619"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..314)
                     /locus_tag="ACHK0Z_RS28125"
                     /old_locus_tag="pgaptmp_005625"
     CDS             complement(<1..314)
                     /locus_tag="ACHK0Z_RS28125"
                     /old_locus_tag="pgaptmp_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspF repeat-containing protein"
                     /protein_id="HGU4505392.1"
                     /translation="MLNGISQAVSTLGRHITSAASRVSSAGLSGFSVSPQAVRLNPIR
                     AQSPFSPGTSNINARTTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKD
                     HLA"
BASE COUNT           88 a           76 c           92 g           66 t
ORIGIN      
        1 gccaaatgat cttttagtgc ctgagcaatg ggcggtaaag ggcgggatgc cccggaagcc
       61 tgtccacttg tcggcggcgg tggggccgga cgagagggag taaatgaagt cgccgggcta
      121 ctcacattaa aagtcgttct cgcattgata ttcgacgtac cgggagaaaa aggagattga
      181 gccctaatcg gattcagacg aacagcctga ggggacactg aaaagccaga taaccccgca
      241 gagcttaccc ggcttgccgc actagtaata tgccgtccta gtgtagaaac agcttgacta
      301 attccattaa gcataattca tg
//
LOCUS       DAZFWP020000610          312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFWP020000610 DAZFWP020000000
VERSION     DAZFWP020000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.620"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>312
                     /locus_tag="ACHK0Z_RS28130"
                     /old_locus_tag="pgaptmp_005626"
     CDS             <1..>312
                     /locus_tag="ACHK0Z_RS28130"
                     /old_locus_tag="pgaptmp_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505393.1"
                     /translation="ILMSRHIRKMQKKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNN
                     IIEIGYNIINTDLVIGEKIHFRTLSNRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNN
                     ET"
BASE COUNT          127 a           62 c           35 g           88 t
ORIGIN      
        1 gatattaatg tcacgacata taagaaagat gcaaaaaaaa ctaaaacaga cgtctgtgcc
       61 tgattttatt tattattcaa tagaaaaaca caacaaatat cccatatatg tattacacat
      121 gccatcaaac aatataattg aaataggcta caatataata aacaccgatc tggtcattgg
      181 cgaaaaaatt catttcagga cactaagcaa tcgttcttta tattttaacc tcacccaacc
      241 cccattaatc gccacaataa aagatgatgt gttttgcact cttcatgatt actataacca
      301 caataatgaa ac
//
LOCUS       DAZFWP020000611          310 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFWP020000611 DAZFWP020000000
VERSION     DAZFWP020000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.621"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>310)
                     /locus_tag="ACHK0Z_RS28135"
                     /old_locus_tag="pgaptmp_005627"
     CDS             complement(<1..>310)
                     /locus_tag="ACHK0Z_RS28135"
                     /old_locus_tag="pgaptmp_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGU4505394.1"
                     /translation="DLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISSVSAD
                     VMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENRCYRA
                     T"
BASE COUNT           71 a          105 c           83 g           51 t
ORIGIN      
        1 gggttgcacg gtaacagcgg ttttccggaa tatagtcctg catttcactg acggatcccg
       61 gggccagcag cattatggct tcacgggcgt cctgacgtat ctgacgcgcc tgcgtcacag
      121 tcccggcata aacgtctatc tgcaccgaca ctgaggactc cgcctgcccg cccatcacgt
      181 ccgccgacac cgatgaaatc aggctgaaaa ccacccacgg aagcgccacc gacggcctgc
      241 catccagcag ggggaccaca tacgggtaca cctgcccgcc ggcaagatgc gccagatgag
      301 gatacaaatc
//
LOCUS       DAZFWP020000612          297 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFWP020000612 DAZFWP020000000
VERSION     DAZFWP020000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.622"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(155..>297)
                     /locus_tag="ACHK0Z_RS28140"
                     /old_locus_tag="pgaptmp_005628"
     CDS             complement(155..>297)
                     /locus_tag="ACHK0Z_RS28140"
                     /old_locus_tag="pgaptmp_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HGU4505395.1"
                     /translation="YRARETDGSASQTFDSGSQGQIQGKITGKDHRCAVGTKMQNGTF
                     CM"
BASE COUNT           59 a           96 c           58 g           84 t
ORIGIN      
        1 ttccctaatc tcacatagga tcagatctcg tctgatcgtt ccccctgcgc tcactaccat
       61 ggctctttac cacagcagcc ggggttgttt gggaccgact tccgacagca gatcccggtg
      121 ggcctgccac catctcttca caagcttatg cttattacat gcaaaacgtc ccgttttgca
      181 tctttgtgcc tacggcacac cgatgatctt tccctgtgat ctttccctga atctgcccct
      241 gagaacctga atcaaacgtc tgactcgcag aaccatctgt ttctcgcgct ctgtaga
//
LOCUS       DAZFWP020000613          292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFWP020000613 DAZFWP020000000
VERSION     DAZFWP020000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.623"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            203..>292
                     /gene="yrhD"
                     /locus_tag="ACHK0Z_RS28145"
                     /old_locus_tag="pgaptmp_005629"
     CDS             203..>292
                     /gene="yrhD"
                     /locus_tag="ACHK0Z_RS28145"
                     /old_locus_tag="pgaptmp_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HGU4505396.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGY"
BASE COUNT          108 a           47 c           48 g           89 t
ORIGIN      
        1 aaaagtaact tttttgaaat tagagataat attggcacag aaaatatatt cagttcgttt
       61 agtgatttct cttcattctt aaacgaaatc atggacagtt gttcataata atcaaaagga
      121 aacaaaaatc attgtaaacc atgccatctt tttagagatt accagcagaa aattccttgt
      181 tcgaaattta gcagtgcaag ctatggaaaa acttacaacg gaactacact ccttaagtga
      241 gatggaccga agacatgtag tctctatttt agccgaaata gctaatggct at
//
LOCUS       DAZFWP020000614          291 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFWP020000614 DAZFWP020000000
VERSION     DAZFWP020000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.624"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>291
                     /locus_tag="ACHK0Z_RS28150"
                     /old_locus_tag="pgaptmp_005630"
     CDS             <1..>291
                     /locus_tag="ACHK0Z_RS28150"
                     /old_locus_tag="pgaptmp_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HGU4505397.1"
                     /translation="LPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLGLSDIS
                     AKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS"
BASE COUNT           89 a           58 c           46 g           98 t
ORIGIN      
        1 acttccgcca attaatattc cctgtacact tttcgaaact attagtttat tcgatgattt
       61 ttctgctgat gatatgcaat atggtgatat ggtagaacaa gactttctct cactcggcct
      121 aagcgatatc tcagcaaaag ttgatcctta cagacttatt aaataccatt tccctggccc
      181 aggttctatt aatgttgctt ttagtgcctc aagttccgga acaaaaatat cacaacgtga
      241 atgcactgat attttatttg cagaaatgaa agaactagca aaaatgtttt c
//
LOCUS       DAZFWP020000615          289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFWP020000615 DAZFWP020000000
VERSION     DAZFWP020000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.625"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>289
                     /locus_tag="ACHK0Z_RS28155"
                     /old_locus_tag="pgaptmp_005631"
     CDS             <1..>289
                     /locus_tag="ACHK0Z_RS28155"
                     /old_locus_tag="pgaptmp_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4505398.1"
                     /translation="MNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGST
                     NRYEGKSFERKDESSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLN"
BASE COUNT          100 a           47 c           62 g           80 t
ORIGIN      
        1 atgaataatc ctgaagggga gcgagcggat ttatctaagg tggaaataga aattacactt
       61 gcctcagctt ttaataatgg gattcgtgaa ggacatacag tcattatatt tacacaacct
      121 gatggttcga ctaatcgtta tgaagggaag tcctttgaaa gaaaagatga gagttcatta
      181 cacctgatta ctaacaagat tctggcgtgt taccaacgcg aagctaacaa ggaaatagct
      241 cgtctattaa atattccgca ggagttaaat aattctcagg acttgaata
//
LOCUS       DAZFWP020000616          288 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFWP020000616 DAZFWP020000000
VERSION     DAZFWP020000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.626"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(125..199)
                     /gene="tnaC"
                     /locus_tag="ACHK0Z_RS28160"
                     /old_locus_tag="pgaptmp_005632"
     CDS             complement(125..199)
                     /gene="tnaC"
                     /locus_tag="ACHK0Z_RS28160"
                     /old_locus_tag="pgaptmp_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGU4505399.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
BASE COUNT          104 a           43 c           54 g           87 t
ORIGIN      
        1 tttactaaaa taagtaaata aacgagagat ggcctttgca aaaggcaaaa ttaagatata
       61 gcaaactaat cgttcacatt gaatctaatc ggtttggctc tggtgatggc tacagaaggg
      121 taaattaagg gcggtgatcg acaatcttat tgtcgatatt gaaccatttt gaggtcacac
      181 atatatgtaa gatattcata atgcacttat cctcgaaaga cacgaggcta cagtataata
      241 ctcaagaatt ttgtctattc taaaattgtt taaatgtgaa tcgaatca
//
LOCUS       DAZFWP020000617          287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFWP020000617 DAZFWP020000000
VERSION     DAZFWP020000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.627"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>287)
                     /locus_tag="ACHK0Z_RS28165"
                     /old_locus_tag="pgaptmp_005633"
     CDS             complement(<1..>287)
                     /locus_tag="ACHK0Z_RS28165"
                     /old_locus_tag="pgaptmp_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4505400.1"
                     /translation="SPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGS
                     GRWERIPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQAT"
BASE COUNT           59 a           99 c           73 g           56 t
ORIGIN      
        1 tgtgttgcct gcagggaatc cagcatcttc agccgttcca tgacgctgta aaactgatta
       61 gccccacggg tctgcccgtc ctccaccggc tcgaaaatat gcagcatggc cggacgcccg
      121 gtgggaagtt cacgcgggat ccgttcccat cgtccactcc cggagcgagg aaaatcatcc
      181 tcacagatat ggtacgcaac ggcacggcca tatcgatcga cctccacccc ggcccgcaga
      241 aaacggttcc ccataccgtg tcctggcgtg tccacccgtt tcggact
//
LOCUS       DAZFWP020000618          287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFWP020000618 DAZFWP020000000
VERSION     DAZFWP020000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.628"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>287
                     /locus_tag="ACHK0Z_RS28170"
                     /old_locus_tag="pgaptmp_005634"
     CDS             <1..>287
                     /locus_tag="ACHK0Z_RS28170"
                     /old_locus_tag="pgaptmp_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGU4505401.1"
                     /translation="EHKIMNNWTRSIKNILTTYLFMSSGAVTARNVQTFSPTINNESR
                     IRLPRALPVGHPYPEEHKASGFSPFMMGGLSGDILPEILTGNGPSIFFNGK"
BASE COUNT           92 a           48 c           63 g           84 t
ORIGIN      
        1 ttgaacataa aattatgaat aattggacaa ggagcataaa aaatatattg acgacatatt
       61 tgtttatgtc atcaggagcg gtgacagcca gaaatgttca aaccttttct ccaacaataa
      121 ataatgagtc aaggattcga ttgccgagag cattgccagt aggccatcca tatcctgagg
      181 aacataaggc ttctggtttc tcccctttta tgatgggggg gctgagtggt gatattcttc
      241 cggaaatttt aacggggaat ggaccatcta tattttttaa cggaaaa
//
LOCUS       DAZFWP020000619          277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFWP020000619 DAZFWP020000000
VERSION     DAZFWP020000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.630"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            121..>277
                     /locus_tag="ACHK0Z_RS28175"
                     /old_locus_tag="pgaptmp_005635"
     CDS             121..>277
                     /locus_tag="ACHK0Z_RS28175"
                     /old_locus_tag="pgaptmp_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4505402.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPR"
BASE COUNT           57 a           69 c           90 g           61 t
ORIGIN      
        1 cacgaacggg cagataggtc cggctgctct gaaggcggtg tatgacatgg cccgcaaggg
       61 tgcccgtgat gaaattcaga cacagatgcg tgatggtggc ctgttctccg gaggtggacg
      121 atgaagacct tccgctggaa agtgaaaccc ggtatggatg tggcttcggt cccttctgta
      181 agaaaggtgc gctttggtga tggctattct cagcgagcgc ctgccgggct gaatgccaac
      241 ctgaaaacgt acagcgtgac gctttctgtc ccccgtg
//
LOCUS       DAZFWP020000620          275 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFWP020000620 DAZFWP020000000
VERSION     DAZFWP020000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.631"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>275
                     /locus_tag="ACHK0Z_RS28180"
                     /old_locus_tag="pgaptmp_005636"
     CDS             <1..>275
                     /locus_tag="ACHK0Z_RS28180"
                     /old_locus_tag="pgaptmp_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGU4505403.1"
                     /translation="TLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQH
                     GMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNI"
BASE COUNT          100 a           37 c           53 g           85 t
ORIGIN      
        1 aaacattaaa tttcgctcat tattatatta tgctcattga gtatattgaa ggtgtcgggt
       61 taaatgagta tttggaaatt tctgaagatc tgaaggatca attatcagaa tcgataaaag
      121 agttgcatca gcatggaatg gtgtctggcg atcctcataa aggaaatttt atagtatccg
      181 aaaaaggttt aagactaatt gacctttcag gtaaaaaaac aaccgctgtt ttaaaagcaa
      241 aagatcgcat tgatcttgaa cggcactata atata
//
LOCUS       DAZFWP020000621          272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFWP020000621 DAZFWP020000000
VERSION     DAZFWP020000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.632"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          108 a           57 c           37 g           70 t
ORIGIN      
        1 aatgttcagg aataaaaaaa agagacaatt cccgagataa aacaaaagag cataaacata
       61 tgaaccggaa attctatttc aaaaatatcc tgtggggaat gtgccccatt ataatatcca
      121 tattgcatct gatatatgac acaccaacag aagcatcgat aaaagttgac ttatttctga
      181 tactcagtgg cctggccctt ccttgttcaa tatacctgac aaaaacaatc acactaaaat
      241 acacatccat atcattctgg aaaaaacctc tt
//
LOCUS       DAZFWP020000622          272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFWP020000622 DAZFWP020000000
VERSION     DAZFWP020000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.633"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            31..>272
                     /locus_tag="ACHK0Z_RS28185"
                     /old_locus_tag="pgaptmp_005637"
     CDS             31..>272
                     /locus_tag="ACHK0Z_RS28185"
                     /old_locus_tag="pgaptmp_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4505404.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           83 a           58 c           57 g           74 t
ORIGIN      
        1 atgcgatcca atcattttaa ggagtttaaa atggataaga agcaagtaac ggatttaagg
       61 tcggaactac tcgattcacg ttttggtgcg aagtctattt ccactatcgc agaatcaaaa
      121 cgttttccgc tgcacgaaat gcgcgacgat gtcgcattcc agattatcaa tgacgaatta
      181 tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa
      241 aatgtccaca aattgatgga tttatccatt aa
//
LOCUS       DAZFWP020000623          272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFWP020000623 DAZFWP020000000
VERSION     DAZFWP020000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.634"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(39..>272)
                     /locus_tag="ACHK0Z_RS28190"
                     /old_locus_tag="pgaptmp_005638"
     CDS             complement(39..>272)
                     /locus_tag="ACHK0Z_RS28190"
                     /old_locus_tag="pgaptmp_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HGU4505405.1"
                     /translation="VQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKA
                     ALALGRRATGVELETERFEQTVREVQDLASQNG"
BASE COUNT           63 a           86 c           59 g           64 t
ORIGIN      
        1 gcgcaggata ataacggtac gtaactaatc ctgcaatatc atccgttctg actggctaaa
       61 tcctgaactt ccctgaccgt ctgctcaaaa cgttcagtct ccagctcaac gccagttgca
      121 cgacgcccca gcgccagtgc agctttgact gtcgaacccg accccatgaa aaaatctgca
      181 accaggtcac ccggacgact gctcgcgctg attatctgct gcagcatttc tgccggtttt
      241 tcgcacggat gtttcccggg atagtactgc ac
//
LOCUS       DAZFWP020000624          267 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFWP020000624 DAZFWP020000000
VERSION     DAZFWP020000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.635"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>267
                     /locus_tag="ACHK0Z_RS28195"
                     /old_locus_tag="pgaptmp_005639"
     CDS             <1..>267
                     /locus_tag="ACHK0Z_RS28195"
                     /old_locus_tag="pgaptmp_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4505406.1"
                     /translation="QFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGV
                     LGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTG"
BASE COUNT           49 a           58 c           94 g           66 t
ORIGIN      
        1 cgcagttcat caggaatctt gccgtggcat cttccggtgt ggcaggtggt gctgtcgggt
       61 cagtggcggg cggaatactg ttcagtccac tgggaccatt tggtgcactg acagggcgtg
      121 tggttggcgg tgttctgggg ggaatgattg cctccgctgt atcaggaaaa attgccggag
      181 cgctggttga agaagatcgc gtcaaaattc tggcaatgat tcaggagcag gtgacatggc
      241 ttgccggcag tttcctgctg accggac
//
LOCUS       DAZFWP020000625          266 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFWP020000625 DAZFWP020000000
VERSION     DAZFWP020000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.636"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>266
                     /gene="bet"
                     /locus_tag="ACHK0Z_RS28200"
                     /old_locus_tag="pgaptmp_005640"
     CDS             <1..>266
                     /gene="bet"
                     /locus_tag="ACHK0Z_RS28200"
                     /old_locus_tag="pgaptmp_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4505407.1"
                     /translation="IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINE
                     NQQFDGMDFEQDNESCTCRIYRKDRNHPICVTEWMDECRREPFKT"
BASE COUNT           70 a           67 c           68 g           61 t
ORIGIN      
        1 atcgcattgt tgatcgtcgc caaccagtac ggccttaatc cgtggacgaa agaaatttac
       61 gccttccctg ataagcagaa cggcatcgtt ccggtggtgg gcgttgatgg ctggtcccgc
      121 atcatcaatg aaaaccagca gtttgatggc atggactttg agcaggacaa tgaatcctgt
      181 acatgccgga tttaccgcaa ggaccgtaat catccgatct gcgttaccga atggatggat
      241 gaatgccgcc gcgaaccatt caaaac
//
LOCUS       DAZFWP020000626          260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFWP020000626 DAZFWP020000000
VERSION     DAZFWP020000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.637"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..186)
                     /locus_tag="ACHK0Z_RS28205"
                     /old_locus_tag="pgaptmp_005641"
     CDS             complement(<1..186)
                     /locus_tag="ACHK0Z_RS28205"
                     /old_locus_tag="pgaptmp_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4505408.1"
                     /translation="MSRKNLWRPLRRLYISASVSVPTKQSQSIPITLAGRTIEKAQEK
                     EGLLVFLGMKSVNDYTLN"
BASE COUNT           69 a           54 c           57 g           80 t
ORIGIN      
        1 attaagagta tagtcattaa cggatttcat tcctaaaaaa acaagtaatc cttctttctc
       61 ttgcgctttt tcgattgttc tccctgctaa agttattggg atggattgag attgttttgt
      121 cggaactgat acgctggcgc taatataaag acggcgcaat ggccgccaca ggttctttct
      181 gctcatccgg cgttatttct acaggtaata attcaacgcc agtgtcagaa gaggttgtta
      241 ccggaagacg ccgcgaaaca
//
LOCUS       DAZFWP020000627          249 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFWP020000627 DAZFWP020000000
VERSION     DAZFWP020000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.638"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..>249
                     /gene="dinJ"
                     /locus_tag="ACHK0Z_RS28210"
                     /old_locus_tag="pgaptmp_005642"
     CDS             81..>249
                     /gene="dinJ"
                     /locus_tag="ACHK0Z_RS28210"
                     /old_locus_tag="pgaptmp_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU4505409.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQ"
BASE COUNT           67 a           60 c           62 g           60 t
ORIGIN      
        1 aaattactca tggattgtcg ctgttgctca tttgagctac aattcaagct gaataaatat
       61 acagcacagg agatacccca atggctgcta acgcatttgt tcgcgcccga atcgatgaag
      121 atctgaagaa tcaggcggcg gacgtactgg ccgggatggg gctgaccatc tctgacctgg
      181 ttcgcataac cctcacaaag gtcgcgcgtg aaaaggcatt gccgtttgat ttacgcgagc
      241 ctaatcaat
//
LOCUS       DAZFWP020000628          249 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFWP020000628 DAZFWP020000000
VERSION     DAZFWP020000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.639"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>249
                     /locus_tag="ACHK0Z_RS28215"
                     /old_locus_tag="pgaptmp_005643"
     CDS             <1..>249
                     /locus_tag="ACHK0Z_RS28215"
                     /old_locus_tag="pgaptmp_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4505410.1"
                     /translation="EQLALVGQNTELAKLKYQTAQGELKTLTEMQKQELLRNAALIDQ
                     QKIREQLRSREETLKNENAAARASNDAELLGYGQGERA"
BASE COUNT           72 a           55 c           88 g           34 t
ORIGIN      
        1 agagcaactg gcactggtgg ggcagaatac agagctggcg aagctgaaat accagactgc
       61 gcagggcgaa ctgaaaaccc tgacggagat gcagaagcag gagctgctgc gtaatgcggc
      121 cctgattgac cagcaaaaaa tccgggaaca gttgcgatcc cgggaagaga cactgaagaa
      181 tgagaatgcg gctgcgcgtg cgtcgaatga tgctgaactg ctggggtacg ggcaggggga
      241 acgagccag
//
LOCUS       DAZFWP020000629          247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFWP020000629 DAZFWP020000000
VERSION     DAZFWP020000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.640"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>247)
                     /locus_tag="ACHK0Z_RS28220"
                     /old_locus_tag="pgaptmp_005644"
     CDS             complement(<1..>247)
                     /locus_tag="ACHK0Z_RS28220"
                     /old_locus_tag="pgaptmp_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4505411.1"
                     /translation="AGQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNAL
                     RDAYDNDTVYAFKVQFPSGKGFKFLAEVRQHTWSSGT"
BASE COUNT           58 a           70 c           63 g           56 t
ORIGIN      
        1 ttggtaccgg atgaccaggt gtgctgacgc acttccgcca ggaacttaaa gcccttaccg
       61 gacggaaact gcaccttaaa cgcatacacc gtgtcattgt cataggcatc acgcagggcg
      121 ttctgggcct gattcagata aaaattaccc gacatggaaa tctcggacga cgcccccaga
      181 ccgttgatgt tctcctgctc tgtggagcag agcgtggtca catcaatatc ctgtttctga
      241 ccggcgg
//
LOCUS       DAZFWP020000630          245 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFWP020000630 DAZFWP020000000
VERSION     DAZFWP020000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.641"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>245
                     /locus_tag="ACHK0Z_RS28225"
                     /old_locus_tag="pgaptmp_005645"
     CDS             <1..>245
                     /locus_tag="ACHK0Z_RS28225"
                     /old_locus_tag="pgaptmp_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU4505412.1"
                     /translation="ADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAGERTFFRRLK
                     EGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLA"
BASE COUNT           54 a           63 c           75 g           53 t
ORIGIN      
        1 tgctgataac cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt
       61 cgcaaacgga atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt
      121 ccggcgttta aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc
      181 gtcgcttgca cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact
      241 tgcca
//
LOCUS       DAZFWP020000631          243 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFWP020000631 DAZFWP020000000
VERSION     DAZFWP020000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.denovo.642"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>243
                     /locus_tag="ACHK0Z_RS28230"
                     /old_locus_tag="pgaptmp_005646"
     CDS             <1..>243
                     /locus_tag="ACHK0Z_RS28230"
                     /old_locus_tag="pgaptmp_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4505413.1"
                     /translation="ESGDRIVRALAAGMGVARKDLKAMADDGKLTADKVVPALISQLG
                     ILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG"
BASE COUNT           60 a           49 c           87 g           47 t
ORIGIN      
        1 atgaaagtgg tgaccggatc gtacgtgcac tggctgcggg tatgggcgtg gcccgtaaag
       61 atctgaaggc aatggcggat gacggaaaac tgacagcgga taaagtggtc cccgcgttaa
      121 tcagccagct ggggatatta cgtgatgaat atgcggccat gccggaaacg gtttccagta
      181 gtatcacaaa ggtggaaaac gcctttatgg cctgggtggg cggtgcgaat gaggccagcg
      241 ggg
//
LOCUS       DAZFWP020000632         8469 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.193, whole genome shotgun sequence.
ACCESSION   DAZFWP020000632 DAZFWP020000000
VERSION     DAZFWP020000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.193"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..112)
                     /locus_tag="ACHK0Z_RS28235"
                     /old_locus_tag="pgaptmp_005647"
     CDS             complement(<1..112)
                     /locus_tag="ACHK0Z_RS28235"
                     /old_locus_tag="pgaptmp_005647"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="poxA; regulates pyruvate oxidase poxB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P lysine(34) lysyltransferase"
                     /protein_id="HGU4505414.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLE"
     gene            437..2245
                     /gene="frdA"
                     /locus_tag="ACHK0Z_RS28240"
                     /old_locus_tag="pgaptmp_005648"
     CDS             437..2245
                     /gene="frdA"
                     /locus_tag="ACHK0Z_RS28240"
                     /old_locus_tag="pgaptmp_005648"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4505415.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2238..2972
                     /gene="frdB"
                     /locus_tag="ACHK0Z_RS28245"
                     /old_locus_tag="pgaptmp_005649"
     CDS             2238..2972
                     /gene="frdB"
                     /locus_tag="ACHK0Z_RS28245"
                     /old_locus_tag="pgaptmp_005649"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4505416.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTNFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2983..3378
                     /gene="frdC"
                     /locus_tag="ACHK0Z_RS28250"
                     /old_locus_tag="pgaptmp_005650"
     CDS             2983..3378
                     /gene="frdC"
                     /locus_tag="ACHK0Z_RS28250"
                     /old_locus_tag="pgaptmp_005650"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4505417.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3389..3748
                     /gene="frdD"
                     /locus_tag="ACHK0Z_RS28255"
                     /old_locus_tag="pgaptmp_005651"
     CDS             3389..3748
                     /gene="frdD"
                     /locus_tag="ACHK0Z_RS28255"
                     /old_locus_tag="pgaptmp_005651"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4505418.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3811..4944
                     /locus_tag="ACHK0Z_RS28260"
                     /old_locus_tag="pgaptmp_005652"
     CDS             3811..4944
                     /locus_tag="ACHK0Z_RS28260"
                     /old_locus_tag="pgaptmp_005652"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4505419.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            5033..5566
                     /gene="blc"
                     /locus_tag="ACHK0Z_RS28265"
                     /old_locus_tag="pgaptmp_005653"
     CDS             5033..5566
                     /gene="blc"
                     /locus_tag="ACHK0Z_RS28265"
                     /old_locus_tag="pgaptmp_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4505420.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5563..5880)
                     /gene="sugE"
                     /locus_tag="ACHK0Z_RS28270"
                     /old_locus_tag="pgaptmp_005654"
     CDS             complement(5563..5880)
                     /gene="sugE"
                     /locus_tag="ACHK0Z_RS28270"
                     /old_locus_tag="pgaptmp_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4505421.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(6062..6208)
                     /gene="ecnB"
                     /locus_tag="ACHK0Z_RS28275"
                     /old_locus_tag="pgaptmp_005655"
     CDS             complement(6062..6208)
                     /gene="ecnB"
                     /locus_tag="ACHK0Z_RS28275"
                     /old_locus_tag="pgaptmp_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4505422.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6319..6444)
                     /gene="ecnA"
                     /locus_tag="ACHK0Z_RS28280"
                     /old_locus_tag="pgaptmp_005656"
     CDS             complement(6319..6444)
                     /gene="ecnA"
                     /locus_tag="ACHK0Z_RS28280"
                     /old_locus_tag="pgaptmp_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4505423.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6496..7062)
                     /gene="efp"
                     /locus_tag="ACHK0Z_RS28285"
                     /old_locus_tag="pgaptmp_005657"
     CDS             complement(6496..7062)
                     /gene="efp"
                     /locus_tag="ACHK0Z_RS28285"
                     /old_locus_tag="pgaptmp_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4505424.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            7104..8132
                     /gene="epmB"
                     /locus_tag="ACHK0Z_RS28290"
                     /old_locus_tag="pgaptmp_005658"
     CDS             7104..8132
                     /gene="epmB"
                     /locus_tag="ACHK0Z_RS28290"
                     /old_locus_tag="pgaptmp_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4505425.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2121 a         2171 c         2200 g         1977 t
ORIGIN      
        1 cctccagcac tccacgatcg gcaaagaaac gacggatctc cgccataatc gccgcgcgtt
       61 ttaataagtt aggaatggat gcgctcggct gccaggatgc cgtttcgctc atagttaaat
      121 ctccagtttt tgacaagggc acgaagtcta ctcgcaacgc gacggcgaga caaattttac
      181 gcaggaatca aacagcggtg ggcagtgact aaaaaaagca cgatctgatg gtttagtaat
      241 taaattaatc atcttcagtg ataatttagc cctcttgcgc actaaaaaaa tcgatctcgt
      301 caaatttcag acttatccat cagactatac tgttgtacct ataaaggagc agtggaatag
      361 cgttcgcaga ccgtaacttt caggcactta ccctgaagta cggggctgtg ggataaaaac
      421 aatctggagg aatgtcgtgc aaacctttca agccgatctt gccattgtag gcgccggtgg
      481 cgcgggatta cgtgctgcaa ttgctgccgc gcaggcaaat ccaaatgcaa aaatcgcact
      541 aatctcaaaa gtatacccga tgcgtagcca taccgttgct gcagaagggg gctccgccgc
      601 tgtcgcgcag gatcatgaca gcttcgaata tcactttcac gatacagtag cgggtggcga
      661 ctggttgtgt gagcaggatg tcgtggatta tttcgtccac cactgcccaa ccgaaatgac
      721 ccaactggaa ctgtgggggt gcccatggag ccgtcgcccg gatggtagcg tcaacgtacg
      781 tcgcttcggc ggcatgaaaa tcgagcgcac ctggttcgcc gccgataaga ccggcttcca
      841 tatgctgcac acgctgttcc agacctctct gcaattcccg cagatccagc gttttgacga
      901 acatttcgtg ctggatattc tggttgatga tggtcatgtt cgcggcgtgg tagcaatgaa
      961 catgatggaa ggcacgctgg tgcagatccg tgctaacgcg gtcgttatgg ctactggcgg
     1021 tgcgggtcgc gtttatcgtt acaacaccaa cggcggcatc gttaccggtg acggtatggg
     1081 tatggcgcta agccacggcg ttccgctgcg tgacatggaa ttcgttcagt atcacccaac
     1141 cggtctgcca ggttccggta tcctgatgac cgaaggctgc cgcggtgaag gcggtattct
     1201 ggtcaacaaa aatggctacc gttatctgca agattacggc atgggcccgg aaactccgct
     1261 gggcgagccg aaaaacaaat atatggaact gggtccacgc gacaaagttt ctcaggcctt
     1321 ctggcacgaa tggcgtaaag gcaacaccat ctccacgccg cgtggcgatg tggtttatct
     1381 cgacttgcgt cacctcggcg agaaaaaact gcatgaacgt ctgccgttca tctgcgaact
     1441 ggcgaaagcg tacgttggcg tcgatccggt taaagaaccg attccggtac gtccgaccgc
     1501 acactacacc atgggcggta tcgaaaccga tcagaactgt gaaacccgca ttaaaggtct
     1561 gttcgccgtg ggtgaatgtt cctctgttgg tctgcacggt gcaaaccgtc tgggttctaa
     1621 ctccctggcg gaactggtgg tcttcggccg tctggccggt gaacaagcga cagagcgtgc
     1681 agcaactgcc ggtaatggca acgaagcggc aattgaagcg caggcagctg gcgttgaaca
     1741 acgtctgaaa gatctggtta accaggatgg cggcgaaaac tgggcgaaga tccgcgacga
     1801 aatgggcctg gcaatggaag aaggttgcgg tatctaccgt acgccggaac tgatgcagaa
     1861 aaccatcgac aagctggcag agctgcagga acgcttcaag cgcgtgcgca tcaccgacac
     1921 ctccagcgtg ttcaacaccg acctgctcta caccattgaa ctgggccacg gtctgaacgt
     1981 tgctgaatgt atggcgcact ccgcaatggc acgtaaagag tcccgcggcg cacaccagcg
     2041 tctggacgaa ggttgcaccg agcgtgacga cgtcaacttc ctcaaacaca ccctcgcctt
     2101 ccgcgatgct gatggcacga ctcgcctgga gtacagcgac gtgaagatta ctacgctgcc
     2161 gccagctaaa cgcgtttacg gtggcgaagc ggatgcagcc gataaggcgg aagcagccaa
     2221 taagaaggag aaggcgaatg gctgagatga aaaacctgaa aattgaggtg gtgcgctata
     2281 acccggaagt cgataccgca ccgcatagcg cattctatga agtgccttat gacgcaacta
     2341 cctcattact ggatgcgctg ggctacatca aagacaacct ggcaccggac ctgagttacc
     2401 gctggtcctg ccgtatggcg atttgtggtt cctgcggcat gatggttaac aacgtgccaa
     2461 aactggcatg taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt gaagcgttag
     2521 ctaacttccc gattgaacgc gatctggtgg tcgatatgac caacttcatc gaaagtctgg
     2581 aagcgatcaa accgtacatc atcggcaact cccgcaccgc ggatcagggt actaacatcc
     2641 agaccccggc gcagatggca aagtatcacc agttctccgg ttgcatcaac tgtggtctgt
     2701 gctacgccgc gtgcccgcag tttggcctga acccagagtt catcggtccg gctgccatta
     2761 cgctggcgca tcgttataac gaagatagcc gcgaccacgg taagaaggag cgtatggcgc
     2821 agttgaacag ccagaacggc gtatggagct gtactttcgt gggctattgc tccgaagtct
     2881 gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa agttcgaaag
     2941 actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac taaacgtaaa
     3001 ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta tcgcttttac
     3061 atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact gattttcggg
     3121 ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt tttacaaaac
     3181 ccggttatcg tgatcattaa cctgatcact ctggcggcag ctctgctgca caccaaaacc
     3241 tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa aatgggacca
     3301 gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt
     3361 gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga
     3421 accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt
     3481 gatgatcctg ctggtgggta ttctgctgcc actggggctg tttccgggtg atgcgctgag
     3541 ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat
     3601 gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa
     3661 aattcacgtg cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt
     3721 cacgctgatt ggtatcgtta caatctaacg tatcgccaat gtaaatccgg cccgcctatg
     3781 gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg cgccttatta
     3841 attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc
     3901 acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat
     3961 cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc
     4021 acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg cgtgcttggt
     4081 ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccacaacaaa atactggcct
     4141 gaacttaccg ctaaacagtg gaatgggatc acactattac atctcgcaac ctacactgct
     4201 ggcggcctgc cattgcaggt gccggatgag gtgaaatcct caagcgactt gctgcgcttc
     4261 tatcaaaact ggcagcctgc atgggcgcca ggaacacaac gtctgtatgc caactccagt
     4321 atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg
     4381 caaacacgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt accgcccgca
     4441 gaagaaaaga attacgcctg gggatatcgc gaaggcaagg cagttcatgt ttcgccaggg
     4501 gcgttagatg ctgaaactta tggtgtgaag tcgaccattg aagatatggc ctgctgggta
     4561 cgaagcaata tgaatccccg tgatatcaac gacaaaacac ttcagcaagg gatacaactg
     4621 gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg
     4681 gactggccgg taaatcctga cagcatcatt aacggcagtg gcaataaaat tgcactggca
     4741 gcacaccccg taaaagcgat tacgccccca actcctgcag tacgcgcatc atgggtacat
     4801 aaaacagggg caaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg
     4861 ggtatagtga tgctggctaa caaaaactat cccaatccag cgagagtcgc cgccgcctgg
     4921 cagattctca acgctctaca gtaaaatttc atcgggtccg aattttcgga ccttttctgc
     4981 gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct
     5041 gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca gttctcctac
     5101 gccgccgcgt ggcgtaacag tagtgaataa tttcgacgcc aaacgttatc tcggtacctg
     5161 gtatgagatt gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag tcaccgcaac
     5221 atacagcctg cgtgatgacg gcggcctgaa tgtcattaat aaaggctata accctgacag
     5281 aggaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa ctcgcgctgc
     5341 gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg cactcgatcg
     5401 ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga tactctcccg
     5461 cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga cccgggaagg
     5521 gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttagtgag tgctgagttt
     5581 cagaccaata atccccaata cgattaacgc cagactcgcc aggcgcatcg ggttagcgga
     5641 ctcaccgagc agcacaatgc cggtgatagc cgcgccgact gcgccaatac ccgtccacac
     5701 ggcataagcc gtccctactg gtaacgattt catcgcccag gcaagtagcg ccatactgac
     5761 aatcatcgcc gtcacggtaa taacactcgg cgtcaaacga ctaaagccgt gggtatattt
     5821 caggccaacg gcccatacca cttccagcag accagcaata actaagataa tccaggacat
     5881 atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg
     5941 caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt
     6001 agtgactatc gcagaaaaaa caaacggcac gacatacctg tcgtaccgtt attgcttatt
     6061 gctattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca gaaatgtctt
     6121 caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa
     6181 agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt
     6241 ttgcctatag caaattaata atagacaaca tcgccaaaaa tgacggaaat ctgatgattt
     6301 ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttgccga gatgtttgat
     6361 gtcttcgccg aaaccgcgag cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac
     6421 aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca gcctgcaggc
     6481 cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt
     6541 gatgacttcg ccgatttgta cgaacagcgg aaccttaacc acagcgccag tagacagggt
     6601 agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatcaacgat
     6661 ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac
     6721 gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt ttgcatcagc
     6781 agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag
     6841 gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag atttgaaggt
     6901 tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc
     6961 tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat
     7021 tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg
     7081 ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag
     7141 aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt
     7201 tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc
     7261 tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac gatcctcttt
     7321 tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc tccaccgacc
     7381 cgctggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac aaccgggcgc
     7441 ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc
     7501 cctatgccga aaatcagggc aacaagcgta actggcagac tgctcttgag tatgttgctg
     7561 cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc
     7621 acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga
     7681 ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct
     7741 ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc aatgaggtag
     7801 atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc
     7861 agagcgttct gttacgtggt gtgaacgata acgcacaaac gctggcaaac ctgagtaatg
     7921 cgttgttcga tgccggcgta atgccgtatt acctgcatgt gctcgataaa gtacagggcg
     7981 cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacac
     8041 tggtgtcggg ttatctggtg ccgaaactgg cgcgagaaat cggcggcgaa cccagcaaaa
     8101 cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt taaacattag
     8161 cctgatgcgc tacgcttatc aggcctacat tgtgcattga aattgaatgg atttgcaatg
     8221 aactgtaggc cggataaggc gtttacgccg catccggcaa gagcaacaaa cgcaagtaca
     8281 gcaacctgtc attaaactgg gaaatttaat ctttgcacgt tgcataacct ttaattattc
     8341 atatgcattg ctgttgttac tttttatata gctaacacca ataattaacc tttcttttct
     8401 gtttatatag agtgagcacg gctatttatg ttattttaac aactattact accaagccca
     8461 tttatctgg
//
LOCUS       DAZFWP020000633         6451 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.224, whole genome shotgun sequence.
ACCESSION   DAZFWP020000633 DAZFWP020000000
VERSION     DAZFWP020000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.224"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..627
                     /gene="dinB"
                     /locus_tag="ACHK0Z_RS28295"
                     /old_locus_tag="pgaptmp_005659"
     CDS             <1..627
                     /gene="dinB"
                     /locus_tag="ACHK0Z_RS28295"
                     /old_locus_tag="pgaptmp_005659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4505426.1"
                     /translation="PVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAKIPGVGKV
                     SAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVGV
                     ERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHV
                     WPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL"
     gene            624..1076
                     /locus_tag="ACHK0Z_RS28300"
                     /old_locus_tag="pgaptmp_005660"
     CDS             624..1076
                     /locus_tag="ACHK0Z_RS28300"
                     /old_locus_tag="pgaptmp_005660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4505427.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            1322..2461
                     /locus_tag="ACHK0Z_RS28305"
                     /old_locus_tag="pgaptmp_005661"
     CDS             1322..2461
                     /locus_tag="ACHK0Z_RS28305"
                     /old_locus_tag="pgaptmp_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4505428.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            2458..3072
                     /gene="prfH"
                     /locus_tag="ACHK0Z_RS28310"
                     /old_locus_tag="pgaptmp_005662"
     CDS             2458..3072
                     /gene="prfH"
                     /locus_tag="ACHK0Z_RS28310"
                     /old_locus_tag="pgaptmp_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4505429.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(3129..4586)
                     /gene="pepD"
                     /locus_tag="ACHK0Z_RS28315"
                     /old_locus_tag="pgaptmp_005663"
     CDS             complement(3129..4586)
                     /gene="pepD"
                     /locus_tag="ACHK0Z_RS28315"
                     /old_locus_tag="pgaptmp_005663"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4505430.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4847..5305
                     /gene="gpt"
                     /locus_tag="ACHK0Z_RS28320"
                     /old_locus_tag="pgaptmp_005664"
     CDS             4847..5305
                     /gene="gpt"
                     /locus_tag="ACHK0Z_RS28320"
                     /old_locus_tag="pgaptmp_005664"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4505431.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            5397..>6451
                     /gene="frsA"
                     /locus_tag="ACHK0Z_RS28325"
                     /old_locus_tag="pgaptmp_005665"
     CDS             5397..>6451
                     /gene="frsA"
                     /locus_tag="ACHK0Z_RS28325"
                     /old_locus_tag="pgaptmp_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4505432.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGR"
BASE COUNT         1554 a         1577 c         1717 g         1603 t
ORIGIN      
        1 ccagtaaagt ttctcgccaa aatcgcctcc gacatgaata aacccaacgg tcagtttgtg
       61 attacgccgg cagaagttcc ggcattttta caaaccttac cgctggcaaa aatccccggc
      121 gtcggcaaag tctcggcggc aaaactggaa gcgatggggc tgcggacctg cggtgatgta
      181 caaaagtgtg atctggtgat gctgcttaaa cgctttggca aatttggccg cattttgtgg
      241 gagcgtagtc aggggattga cgagcgcgac gttaacagcg aacggttgcg aaaatccgtc
      301 ggcgtggaac gcacgatggc ggaagatatc caccactggt ctgaatgtga agcgattatc
      361 gagcggctgt atccggaact tgaacgccgt ctggcaaagg taaaacctga tttactgatt
      421 gcccgccagg gggtgaaatt aaagttcgac gattttcagc aaaccactca ggagcacgtc
      481 tggccgcggc tgaataaagc tgacttaatc gccaccgcgc gtaaaacctg ggatgaacgc
      541 cgcggcgggc gcggtgtgcg actggtgggg ctgcatgtga cgttgcttga cccgcaaatg
      601 gaaagacaac tggtgctggg attatgaata acatacaaat aagagactat cagcctggcg
      661 attttcagca actatgcgct attttcatta gagcggttac gatgactgcc agtcagcatt
      721 attcaccaca acaaattgcc gcctgggcgc agattgacga atctcgctgg aaggagaaac
      781 tcgcgaaatc acaagtgcgg gttgcagtca ttaatgcaaa actggtcggc tttattacct
      841 gcgttgaaca ctatatcgat atgttatttg ttgaccctga atacacccgc cgtggggttg
      901 ccagcgcttt gttaaaacct ttgattaagt ctgaatccga acttacggtg gacgcaagca
      961 taaccgcaaa accctttttt gaacgttatg gttttcagac agttaagcag caacgcgttg
     1021 aatgtcgggg agagtggttt attaattttt atatgcgtta taaacagcaa cattaaatcc
     1081 agcttgcaat gaaaataacg caagcctagt atgtgcgcgt tttcttattc cacaaactgc
     1141 aaggaggtaa atcatgacaa atcctttatc aatgactctt tgcagacctt tccaggatta
     1201 attctttttt tcttgccctg gattcgtctg ccatttcctg attttttaat tgttactctc
     1261 aataattcaa gtcgcaacac atgcaacttg aagtatgacg agtccattta cggaatggta
     1321 tatgggcaat tatattcgtc ccttatccga tgcggtattt accatcgcat ctgatgacct
     1381 gtggatcgag agtttagcga tccaacaatt acacaccacg gcaaatttac ccaacatgca
     1441 gcgcgtagtg gggatgccag atttacatcc cggacgcggc tatccgattg gcgcagcgtt
     1501 cttctctgtt ggtcgttttt atccggcact ggtcggcaat gatatcggct gcggtatggc
     1561 gctatggcaa acagatattc tcgctcgcaa atacaacgcc gataagtttg aaaagcgatt
     1621 atcagatctg gatgacgttg ctgaagaaag ctggctggag gaaaacctgc cgtcagcatt
     1681 tgcacagcat ccgtggcgca gctcgcttgg ctctatcggt ggcggcaatc atttcgctga
     1741 actgcaacaa gttgatcaaa tcatcaacgc tgaactgttt gcactggcag gtctggatgc
     1801 acaacatctg caactgttgg ttcatagcgg ctcgcggggg gtggggcagt ctattttaca
     1861 gcggcatatt gcctcgttct cgcatcatgg tttgcctcaa ggcagtgacg acgcgctacg
     1921 gtatattgcg gaacatgacg atgcgctggc gtttgcgtgt attaatcgcc agatgatcgc
     1981 tttgcgcata atgcaacaag ttaaggccac gggaagtccg gttctggatg tggcgcataa
     2041 ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta aaggtgccac
     2101 gccggatgac aacggtctgg tgattattcc cggttcacgt ggtgattact cctggattgt
     2161 acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggcag ggcgtaaatg
     2221 ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acggcgacgc aactctcgcg
     2281 tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg aagaagcgcc
     2341 acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggccg gtttaattat
     2401 tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga aaaaaggatg
     2461 atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc agtgaaaaag
     2521 gcgctggaca ggcttattaa agaagctgcc cgacaagacg tcgcggtaac ggtgttggaa
     2581 acagaaacgg gccgctactc tgacacgctg cgttcggcgc tgatttctct ggatggcgat
     2641 aacgcatggg cattaagcga aagctggtgt ggcgctattc agtggatttg tccgagtccg
     2701 tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt taccgctgat
     2761 gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg gccgggcggt
     2821 caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc cggtattagc
     2881 gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg attgctgatt
     2941 gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc gcagcggcga
     3001 atgttccatc accagattga acgtggcaac ccgagacgga catttacagg gatggctttt
     3061 atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa
     3121 tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagcgt ccagtaatga
     3181 cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat agttggcccg
     3241 atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc caggcccgcg
     3301 tggataatct ggatgttcgg tgttttgttg aacagacgct gataggtttc acgtaccaga
     3361 tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt cgcttcggtt
     3421 ttcgcgccag ccagtttacc cagcgaatcc agcatgctca cgacgtagtc tttaccgctg
     3481 tcgatcagtg aacggatcag acagtgaatt tctacgttat tatcagtcat ggtcaccaca
     3541 ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg gatcacaccg
     3601 ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat cagggcagct
     3661 ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc tgccagctcg
     3721 tttttcagga tctcctgata ggtattcatc agagatttca ggacgtcgac tttatcagct
     3781 gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg
     3841 aaatcgataa ggcgcagatc cagttcttct gcatgacccg ccaggaagcg caccagcagt
     3901 ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc tttcagacct
     3961 tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg
     4021 ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag
     4081 tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc
     4141 ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac cacgttttcg
     4201 tcagccagaa ctgccagtgc agaggccata ccaatgccgt tatccgcacc cagcgtggtg
     4261 ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca
     4321 tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt
     4381 ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg
     4441 cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct
     4501 tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca cagcggctgt
     4561 ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt
     4621 attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg
     4681 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     4741 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     4801 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     4861 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     4921 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt
     4981 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     5041 cgacaaccag cgcgagctta aagtgctgaa acgtgcagaa ggcgatggcg aaggcttcat
     5101 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     5161 aaaagcgcac tttgtcacca tcttcgcaaa accggccggt cgcccgctgg ttgatgatta
     5221 tgttgttgat atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt
     5281 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     5341 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     5401 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     5461 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta
     5521 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg
     5581 acccacgtga aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg
     5641 acgatgattt atacgatact gtgattggct accgtggtgg caactggatt tatgagtggg
     5701 ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagtg
     5761 gtcgacactg gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag
     5821 gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc
     5881 agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca
     5941 ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg
     6001 gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg
     6061 gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca
     6121 cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg
     6181 tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc
     6241 tggcatacct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata
     6301 ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg
     6361 cgagtcgttt ggggatgcat gatgcttccg atgacgcgtt gcgcgtggag ctgaatcgct
     6421 attcattaaa agtgcaagga ttgctgggac g
//
LOCUS       DAZFWP020000634         2077 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.338, whole genome shotgun sequence.
ACCESSION   DAZFWP020000634 DAZFWP020000000
VERSION     DAZFWP020000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.338"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            400..1347
                     /gene="stxA1a"
                     /locus_tag="ACHK0Z_RS28330"
                     /old_locus_tag="pgaptmp_005666"
     CDS             400..1347
                     /gene="stxA1a"
                     /locus_tag="ACHK0Z_RS28330"
                     /old_locus_tag="pgaptmp_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4505433.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1357..1626
                     /gene="stxB1a"
                     /locus_tag="ACHK0Z_RS28335"
                     /old_locus_tag="pgaptmp_005667"
     CDS             1357..1626
                     /gene="stxB1a"
                     /locus_tag="ACHK0Z_RS28335"
                     /old_locus_tag="pgaptmp_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HGU4505434.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          553 a          376 c          503 g          645 t
ORIGIN      
        1 gaaaggaagg tgcgaccaca attaataaca aaatcttaaa aattgcacat ggcactatta
       61 gttttctaaa tattgtgtat tttttgtatt gcaggatgac cctgtaacga agtttgcgta
      121 acagcatttt gctctacgag tttgccagcc tcccccagtg gctggctttt ttatgtccgt
      181 aacatcctgt gtatcaataa atgttgttat ctacgtacgt caagtagtcg catgagatct
      241 gaccagatat gttaaggttg cagctctctt tgaatatgat tatcattttc attacgttat
      301 tgttacgttt atccggtgcg ccgtaaaacg ccgtccttca gggcgtggag gatgtcaaga
      361 atatagttat cgtatggtgc tcaaggagta ttgtgtaata tgaaaataat tatttttaga
      421 gtgctaactt ttttctttgt tatcttttca gttaatgtgg tggcgaagga atttacctta
      481 gacttctcga ctgcaaagac gtatgtagat tcgctgaatg tcattcgctc tgcaataggt
      541 actccattac agactatttc atcaggaggt acgtctttac tgatgattga tagtggcaca
      601 ggggataatt tgtttgcagt tgatgtcaga gggatagatc cagaggaagg gcggtttaat
      661 aatctacggc ttattgttga acgaaataat ttatatgtga caggatttgt taacaggaca
      721 aataatgttt tttatcgctt tgctgatttt tcacatgtta cctttccagg tacaacagcg
      781 gttacattgt ctggtgacag tagctatacc acgttacagc gtgttgcagg gatcagtcgt
      841 acggggatgc agataaatcg ccattcgttg actacttctt atctggattt aatgtcgcat
      901 agtggaacct cactgacgca gtctgtggca agagcgatgt tacggtttgt tactgtgaca
      961 gctgaagctt tacgttttcg gcaaatacag aggggatttc gtacaacact ggatgatctc
     1021 agtgggcgtt cttatgtaat gactgctgaa gatgttgatc ttacattgaa ctggggaagg
     1081 ttgagtagcg tcctgcctga ctatcatgga caagactctg ttcgtgtagg aagaatttct
     1141 tttggaagca ttaatgcaat tctgggaagc gtggcattaa tactgaattg tcatcatcat
     1201 gcatcgcgag ttgccagaat ggcatctgat gagtttcctt ctatgtgtcc ggcagatgga
     1261 agagtccgtg ggattacgca caataaaata ttgtgggatt catccactct gggggcaatt
     1321 ctgatgcgca gaactattag cagttgaggg ggtaaaatga aaaaaacatt attaatagct
     1381 gcatcgcttt catttttttc agcaagtgcg ctggcgacgc ctgattgtgt aactggaaag
     1441 gtggagtata caaaatataa tgatgacgat acctttacag ttaaagtggg tgataaagaa
     1501 ttatttacca acagatggaa tcttcagtct cttcttctca gtgcgcaaat tacggggatg
     1561 actgtaacca ttaaaactaa tgcctgtcat aatggagggg gattcagcga agttattttt
     1621 cgttgactca gaatagctca gtgaaaatag caggcggaga ttcataaatg ttaaatacat
     1681 ctcaattcag tcagttgttg ccggtctgat aatagatgtg ttagaaaatt tctgcatggt
     1741 gaatccccct gtgcggaggg gcgactggtg aacggtatga tctctttgat gatcgtaagc
     1801 gagaatacgc gggtttggtg gcaccaggcc gaactcaccg ggaggcaccc ggcatcatgc
     1861 tgtatacaga gattaggcat atatccaggc tcctcatcgc aggagccttt ttacatgcaa
     1921 aaaaaagccc gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt
     1981 gcggatttta accaggattc ataaggctgc gcaactgcgc ggcctttttc gtatttcggg
     2041 ctgtagtccc cgtgtgtcat tcaggcttcc ggactac
//
LOCUS       DAZFWP020000635         1473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.384, whole genome shotgun sequence.
ACCESSION   DAZFWP020000635 DAZFWP020000000
VERSION     DAZFWP020000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.384"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..1079
                     /locus_tag="ACHK0Z_RS28340"
                     /old_locus_tag="pgaptmp_005668"
     CDS             120..1079
                     /locus_tag="ACHK0Z_RS28340"
                     /old_locus_tag="pgaptmp_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4505435.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHK0Z_RS28345"
                     /old_locus_tag="pgaptmp_005669"
     CDS             1091..1360
                     /locus_tag="ACHK0Z_RS28345"
                     /old_locus_tag="pgaptmp_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4505436.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          402 a          293 c          358 g          420 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacaccccg ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccga tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcacg gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cagcgatact gggtactgtg gccgttatac
      901 tgaattgcca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagcagc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgcgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagctga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaa
//
LOCUS       DAZFWP020000636         1473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.385, whole genome shotgun sequence.
ACCESSION   DAZFWP020000636 DAZFWP020000000
VERSION     DAZFWP020000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.385"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..1079
                     /locus_tag="ACHK0Z_RS28350"
                     /old_locus_tag="pgaptmp_005670"
     CDS             120..1079
                     /locus_tag="ACHK0Z_RS28350"
                     /old_locus_tag="pgaptmp_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4505437.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHK0Z_RS28355"
                     /old_locus_tag="pgaptmp_005671"
     CDS             1091..1360
                     /locus_tag="ACHK0Z_RS28355"
                     /old_locus_tag="pgaptmp_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4505438.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          402 a          291 c          358 g          422 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacaccccg ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccga tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcacg gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cagcgatact gggtactgtg gccgttatac
      901 tgaattgcca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagcagc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgtgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaa
//
LOCUS       DAZFWP020000637         1473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.386, whole genome shotgun sequence.
ACCESSION   DAZFWP020000637 DAZFWP020000000
VERSION     DAZFWP020000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.386"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..1079
                     /locus_tag="ACHK0Z_RS28360"
                     /old_locus_tag="pgaptmp_005672"
     CDS             120..1079
                     /locus_tag="ACHK0Z_RS28360"
                     /old_locus_tag="pgaptmp_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4505439.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHK0Z_RS28365"
                     /old_locus_tag="pgaptmp_005673"
     CDS             1091..1360
                     /locus_tag="ACHK0Z_RS28365"
                     /old_locus_tag="pgaptmp_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4505440.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          401 a          293 c          358 g          421 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacaccccg ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccga tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcacg gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cagcgatact gggtactgtg gccgttatac
      901 tgaattgcca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagctgc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgcgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagctga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaa
//
LOCUS       DAZFWP020000638         1473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.387, whole genome shotgun sequence.
ACCESSION   DAZFWP020000638 DAZFWP020000000
VERSION     DAZFWP020000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.387"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..1079
                     /locus_tag="ACHK0Z_RS28370"
                     /old_locus_tag="pgaptmp_005674"
     CDS             120..1079
                     /locus_tag="ACHK0Z_RS28370"
                     /old_locus_tag="pgaptmp_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4505441.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHK0Z_RS28375"
                     /old_locus_tag="pgaptmp_005675"
     CDS             1091..1360
                     /locus_tag="ACHK0Z_RS28375"
                     /old_locus_tag="pgaptmp_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4505442.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          401 a          291 c          358 g          423 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacaccccg ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccga tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcacg gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cagcgatact gggtactgtg gccgttatac
      901 tgaattgcca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagctgc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgtgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaa
//
LOCUS       DAZFWP020000639         1473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.388, whole genome shotgun sequence.
ACCESSION   DAZFWP020000639 DAZFWP020000000
VERSION     DAZFWP020000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.388"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..1079
                     /locus_tag="ACHK0Z_RS28380"
                     /old_locus_tag="pgaptmp_005676"
     CDS             120..1079
                     /locus_tag="ACHK0Z_RS28380"
                     /old_locus_tag="pgaptmp_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4505443.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHK0Z_RS28385"
                     /old_locus_tag="pgaptmp_005677"
     CDS             1091..1360
                     /locus_tag="ACHK0Z_RS28385"
                     /old_locus_tag="pgaptmp_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4505444.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          401 a          294 c          359 g          419 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga gtttacgata gacttttcga cccaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacacccca ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccgg tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcaca gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cggcgatact gggcactgtg gccgttatac
      901 tgaattgtca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagcagc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgcgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagctga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaa
//
LOCUS       DAZFWP020000640         1473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2212S50119
            SAMN43283591-rid25106983.guided.389, whole genome shotgun sequence.
ACCESSION   DAZFWP020000640 DAZFWP020000000
VERSION     DAZFWP020000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283591
            Sequence Read Archive: SRR30325295
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351557.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351557.2
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:19:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,679
            CDSs (total)                      :: 5,591
            Genes (coding)                    :: 5,357
            CDSs (with protein)               :: 5,357
            Genes (RNA)                       :: 88
            rRNAs                             :: 4, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 101 of 234
            Pseudo Genes (incomplete)         :: 129 of 234
            Pseudo Genes (internal stop)      :: 52 of 234
            Pseudo Genes (multiple problems)  :: 43 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283591-rid25106983.guided.389"
                     /serotype="O103:H 2"
                     /isolate="2212S50119"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            120..1079
                     /locus_tag="ACHK0Z_RS28390"
                     /old_locus_tag="pgaptmp_005678"
     CDS             120..1079
                     /locus_tag="ACHK0Z_RS28390"
                     /old_locus_tag="pgaptmp_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4505445.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1091..1360
                     /locus_tag="ACHK0Z_RS28395"
                     /old_locus_tag="pgaptmp_005679"
     CDS             1091..1360
                     /locus_tag="ACHK0Z_RS28395"
                     /old_locus_tag="pgaptmp_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4505446.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          401 a          292 c          359 g          421 t
ORIGIN      
        1 cactggttcg aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc
       61 ttttttatat ctgcgccggg tctggtgctg attacttcag ccaaaaggaa cacctgtata
      121 tgaagtgtat attatttaaa tgggtactgt gcctgttact gggtttttct tcggtatcct
      181 attcccggga gtttacgata gacttttcga cccaacaaag ttatgtctct tcgttaaata
      241 gtatacggac agagatatcg acccctcttg aacatatatc tcaggggacc acatcggtgt
      301 ctgttattaa ccacacccca ccgggcagtt attttgctgt ggatatacga gggcttgatg
      361 tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg
      421 ccgggttcgt taatacggca acaaatactt tctaccgttt ttcagatttt acacatatat
      481 cagtgcccgg tgtgacaacg gtttccatga caacggacag cagttatacc actctgcaac
      541 gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat
      601 atctggcgtt aatggagttc agtggtaata caatgaccag agatgcatcc agagcagttc
      661 tgcgttttgt cactgtcaca gcagaagcct tacgcttcag gcagatacag agagaatttc
      721 gtcaggcact gtctgaaact gctcctgtgt atacgatgac gccgggagac gtggacctca
      781 ctctgaactg ggggcgaatc agcaatgtgc ttccggagta tcggggagag gatggtgtca
      841 gagtggggag aatatccttt aataatatat cggcgatact gggcactgtg gccgttatac
      901 tgaattgtca tcatcagggg gcgcgttctg ttcgcgccgt gaatgaagag agtcaaccag
      961 aatgtcagat aactggcgac aggcccgtta taaaaataaa caatacatta tgggaaagta
     1021 atacagcagc agcgtttctg aacagaaagt cacagttttt atatacaacg ggtaaataaa
     1081 ggagttaagt atgaagaaga tgtttatggc ggttttattt gcattagttt ctgttaatgc
     1141 aatggcggcg gattgtgcta aaggtaaaat tgagttttcc aagtataatg aggatgacac
     1201 atttacagtg aaggttgacg ggaaagaata ctggaccagt cgctggaatc tgcaaccgtt
     1261 actgcaaagt gctcagttga caggaatgac tgtcacaatc aaatccagta cctgtgaatc
     1321 aggctccgga tttgctgaag tgcagtttaa taatgactga ggcataacct gattcgtggt
     1381 atgtgggtaa caagtgtaat ctgtgtcaca attcagtcag ttgacagttg cctgtcagac
     1441 tgagcatttg ttaaaaaaat ttcgcatggt gaa
//
